tacoma-olympia section asce: Topics by Science.gov

Sample records for tacoma-olympia section asce

High-resolution seismic reflection imaging of growth folding and shallow faults beneath the Southern Puget Lowland, Washington State

USGS Publications Warehouse

Clement, C.R.; Pratt, T.L.; Holmes, M.L.; Sherrod, B.L.

2010-01-01

Marine seismic reflection data from southern Puget Sound, Washington, were collected to investigate the nature of shallow structures associated with the Tacoma fault zone and the Olympia structure. Growth folding and probable Holocene surface deformation were imaged within the Tacoma fault zone beneath Case and Carr Inlets. Shallow faults near potential field anomalies associated with the Olympia structure were imaged beneath Budd and Eld Inlets. Beneath Case Inlet, the Tacoma fault zone includes an ???350-m wide section of south-dipping strata forming the upper part of a fold (kink band) coincident with the southern edge of an uplifted shoreline terrace. An ???2 m change in the depth of the water bottom, onlapping postglacial sediments, and increasing stratal dips with increasing depth are consistent with late Pleistocene to Holocene postglacial growth folding above a blind fault. Geologic data across a topographic lineament on nearby land indicate recent uplift of late Holocene age. Profiles acquired in Carr Inlet 10 km to the east of Case Inlet showed late Pleistocene or Holocene faulting at one location with ???3 to 4 m of vertical displacement, south side up. North of this fault the data show several other disruptions and reflector terminations that could mark faults within the broad Tacoma fault zone. Seismic reflection profiles across part of the Olympia structure beneath southern Puget Sound show two apparent faults about 160 m apart having 1 to 2 m of displacement of subhorizontal bedding. Directly beneath one of these faults, a dipping reflector that may mark the base of a glacial channel shows the opposite sense of throw, suggesting strike-slip motion. Deeper seismic reflection profiles show disrupted strata beneath these faults but little apparent vertical offset, consistent with strike-slip faulting. These faults and folds indicate that the Tacoma fault and Olympia structure include active structures with probable postglacial motion.
High-resolution seismic reflection imaging of growth folding and shallow faults beneath the Southern Puget Lowland, Washington State

USGS Publications Warehouse

Odum, Jackson K.; Stephenson, William J.; Pratt, Thomas L.; Blakely, Richard J.

2016-01-01

Marine seismic reflection data from southern Puget Sound, Washington, were collected to investigate the nature of shallow structures associated with the Tacoma fault zone and the Olympia structure. Growth folding and probable Holocene surface deformation were imaged within the Tacoma fault zone beneath Case and Carr Inlets. Shallow faults near potential field anomalies associated with the Olympia structure were imaged beneath Budd and Eld Inlets. Beneath Case Inlet, the Tacoma fault zone includes an ∼350-m wide section of south-dipping strata forming the upper part of a fold (kink band) coincident with the southern edge of an uplifted shoreline terrace. An ∼2 m change in the depth of the water bottom, onlapping postglacial sediments, and increasing stratal dips with increasing depth are consistent with late Pleistocene to Holocene postglacial growth folding above a blind fault. Geologic data across a topographic lineament on nearby land indicate recent uplift of late Holocene age. Profiles acquired in Carr Inlet 10 km to the east of Case Inlet showed late Pleistocene or Holocene faulting at one location with ∼3 to 4 m of vertical displacement, south side up. North of this fault the data show several other disruptions and reflector terminations that could mark faults within the broad Tacoma fault zone. Seismic reflection profiles across part of the Olympia structure beneath southern Puget Sound show two apparent faults about 160 m apart having 1 to 2 m of displacement of subhorizontal bedding. Directly beneath one of these faults, a dipping reflector that may mark the base of a glacial channel shows the opposite sense of throw, suggesting strike-slip motion. Deeper seismic reflection profiles show disrupted strata beneath these faults but little apparent vertical offset, consistent with strike-slip faulting. These faults and folds indicate that the Tacoma fault and Olympia structure include active structures with probable postglacial motion.
Public health assessment for Commencement Bay, South Tacoma Field, Tacoma, Pierce County, Washington, Region 10. Cerclis No. WAD980724173 (A/K/A Commencement Bay, South Tacoma Channel, Tacoma, Pierce County, Washington, Region 10. Cerclis No. WAD980726301). Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

The Commencement Bay, South Tacoma Field site occupies 260 acres of land located in the southwestern section of the city of Tacoma in Pierce County, Washington. The South Tacoma Field site poses a public health hazard to trespassers who repeatedly ingest contaminated surface soils, surface water, and sediment during recreational activities at the site. Exposure to the contaminants arsenic, copper, lead, manganese, as well as polychlorinated biphenyl and polycyclic aromatic hydrocarbon compounds may have occurred in the past, may be presently occurring, and may occur in the future, which could result in noncarcinogenic and carcinogenic health effects. Potentially, the sitemore » can pose a public health hazard through exposure to groundwater and subsurface soil contaminants that could cause adverse health effects. Additionally, should contruction/excavation uncover contaminated subsurface soils, workers as well as recreationalists/trespassers may be exposed.« less
21 CFR 161.136 - Olympia oysters.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Olympia oysters. 161.136 Section 161.136 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN... and conform to the definition and standard of identity prescribed for oysters in § 161.130. ...
21 CFR 161.136 - Olympia oysters.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Olympia oysters. 161.136 Section 161.136 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN... and conform to the definition and standard of identity prescribed for oysters in § 161.130. ...
33 CFR 100.1309 - Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA. 100.1309 Section 100.1309 Navigation and Navigable Waters... WATERS § 100.1309 Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA. (a...
33 CFR 100.1309 - Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA. 100.1309 Section 100.1309 Navigation and Navigable Waters... WATERS § 100.1309 Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA. (a...
33 CFR 100.1309 - Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA. 100.1309 Section 100.1309 Navigation and Navigable Waters... WATERS § 100.1309 Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA. (a...
33 CFR 100.1309 - Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA. 100.1309 Section 100.1309 Navigation and Navigable Waters... WATERS § 100.1309 Special Local Regulation; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA. (a...
75 FR 80813 - Appraisal Subcommittee (ASC); ASC Rules of Operation; Amended

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-23

... name and must rely on the General Services Administration (GSA) for the procurement of office space by... administration, procurement, and other services, consistent with directives of the ASC. In executing this... Conduct Section 11.01. Ethics Provision. The ASC members of the ASC and its officers and employees shall...
A Negotiated Agreement between Tacoma Community College Federation of Teachers and Tacoma Community College, District XXII.

ERIC Educational Resources Information Center

Tacoma Community Coll., WA.

The collective bargaining agreement between the Tacoma Community College Federation of Teachers and Tacoma Community College, District XXII is presented. This contract, covering the period from September 1, 1988 to June 30, 1990, deals with the following topics: the purpose, definitions, and conditions of the agreement; union rights and…
Investigating Mars: Olympia Undae

NASA Image and Video Library

2018-03-12

This VIS image of Olympia Undae was collected during north polar summer. The dunes are now completely frost free and are dark in color due to being made of basaltic sand. The surface between the dunes, where visible, is a bright tone. In some regions of dense dunes, the bright material may be a deposit on the dunes rather than the underlying surface. The presence of gypsum has been suggested for Olympia Undae, gypsum is a lighter tone than basalt in this filter of the THEMIS VIS camera. Olympia Undae is a vast dune field in the north polar region of Mars. It consists of a broad sand sea or erg that partly rings the north polar cap from about 120° to 240°E longitude and 78° to 83°N latitude. The dune field covers an area of approximately 470,000 km2 (bigger than California, smaller than Texas). Olympia Undae is the largest continuous dune field on Mars. Olympia Undae is not the only dune field near the north polar cap, several other smaller fields exist in the same latitude, but in other ranges of longitude, e.g. Abolos and Siton Undae. Barchan and transverse dune forms are the most common. In regions with limited available sand individual barchan dunes will form, the surface beneath and between the dunes is visible. In regions with large sand supplies, the sand sheet covers the underlying surface, and dune forms are found modifying the surface of the sand sheet. In this case transverse dunes are more common. Barchan dunes "point" down wind, transverse dunes are more linear and form parallel to the wind direction. The "square" shaped transverse dunes in Olympia Undae are due to two prevailing wind directions. The density of dunes and the alignments of the dune crests varies with location, controlled by the amount of available sand and the predominant winds over time. The Odyssey spacecraft has spent over 15 years in orbit around Mars, circling the planet more than 71,000 times. It holds the record for longest working spacecraft at Mars. THEMIS, the IR/VIS camera
Investigating Mars: Olympia Undae

NASA Image and Video Library

2018-03-13

This VIS image of Olympia Undae was collected during north polar summer. The dunes are now completely frost free and are dark in color due to being made of basaltic sand. The surface between the dunes, where visible, is a bright tone. In some regions of dense dunes, the bright material may be a deposit on the dunes rather than the underlying surface. The presence of gypsum has been suggested for Olympia Undae, gypsum is a lighter tone than basalt in this filter of the THEMIS VIS camera. Olympia Undae is a vast dune field in the north polar region of Mars. It consists of a broad sand sea or erg that partly rings the north polar cap from about 120° to 240°E longitude and 78° to 83°N latitude. The dune field covers an area of approximately 470,000 km2 (bigger than California, smaller than Texas). Olympia Undae is the largest continuous dune field on Mars. Olympia Undae is not the only dune field near the north polar cap, several other smaller fields exist in the same latitude, but in other ranges of longitude, e.g. Abolos and Siton Undae. Barchan and transverse dune forms are the most common. In regions with limited available sand individual barchan dunes will form, the surface beneath and between the dunes is visible. In regions with large sand supplies, the sand sheet covers the underlying surface, and dune forms are found modifying the surface of the sand sheet. In this case transverse dunes are more common. Barchan dunes "point" down wind, transverse dunes are more linear and form parallel to the wind direction. The "square" shaped transverse dunes in Olympia Undae are due to two prevailing wind directions. The density of dunes and the alignments of the dune crests varies with location, controlled by the amount of available sand and the predominant winds over time. The Odyssey spacecraft has spent over 15 years in orbit around Mars, circling the planet more than 71,000 times. It holds the record for longest working spacecraft at Mars. THEMIS, the IR/VIS camera
Investigating Mars: Olympia Undae

NASA Image and Video Library

2018-03-06

This VIS image of Olympia Undae was collected early in north polar spring. The crests of the dunes are light colored, indicative of a frost covering. As the season changes into summertime, the dune crests will lose the frost and reveal the darker sand beneath. The margin of the north polar cap is visible at the top of the image. Olympia Undae is a vast dune field in the north polar region of Mars. It consists of a broad sand sea or erg that partly rings the north polar cap from about 120° to 240°E longitude and 78° to 83°N latitude. The dune field covers an area of approximately 470,000 km2 (bigger than California, smaller than Texas). Olympia Undae is the largest continuous dune field on Mars. Olympia Undae is not the only dune field near the north polar cap, several other smaller fields exist in the same latitude, but in other ranges of longitude, e.g. Abolos and Siton Undae. Barchan and transverse dune forms are the most common. In regions with limited available sand individual barchan dunes will form, the surface beneath and between the dunes is visible. In regions with large sand supplies, the sand sheet covers the underlying surface, and dune forms are found modifying the surface of the sand sheet. In this case transverse dunes are more common. Barchan dunes "point" down wind, transverse dunes are more linear and form parallel to the wind direction. The "square" shaped transverse dunes in Olympia Undae are due to two prevailing wind directions. The Odyssey spacecraft has spent over 15 years in orbit around Mars, circling the planet more than 71,000 times. It holds the record for longest working spacecraft at Mars. THEMIS, the IR/VIS camera system, has collected data for the entire mission and provides images covering all seasons and lighting conditions. Over the years many features of interest have received repeated imaging, building up a suite of images covering the entire feature. From the deepest chasma to the tallest volcano, individual dunes inside
Investigating Mars: Olympia Undae

NASA Image and Video Library

2018-03-14

This VIS image of Olympia Undae was collected during north polar summer. The dunes are now completely frost free and are dark in color due to being made of basaltic sand. The surface between the dunes, where visible, is a bright tone. In some regions of dense dunes, the bright material may be a deposit on the dunes rather than the underlying surface. The presence of gypsum has been suggested for Olympia Undae, gypsum is a lighter tone than basalt in this filter of the THEMIS VIS camera. This VIS image hightlights the density of dunes, the bottom third of the image has fewer dunes, spaced farther apart than the top two thirds of the image. The bottom of the image "looks" like lace, while the top with the dense dunes with aligned dune crests "looks" like waves in an ocean. The term used for dune fields on Mar is undae (unda singular). This term translates from Latin as water waves and is used to mean undulatory in planetary nomenclature. All non-Earth dune fields in the solar system are called unda/undae. Olympia Undae is a vast dune field in the north polar region of Mars. It consists of a broad sand sea or erg that partly rings the north polar cap from about 120° to 240°E longitude and 78° to 83°N latitude. The dune field covers an area of approximately 470,000 km2 (bigger than California, smaller than Texas). Olympia Undae is the largest continuous dune field on Mars. Olympia Undae is not the only dune field near the north polar cap, several other smaller fields exist in the same latitude, but in other ranges of longitude, e.g. Abolos and Siton Undae. Barchan and transverse dune forms are the most common. In regions with limited available sand individual barchan dunes will form, the surface beneath and between the dunes is visible. In regions with large sand supplies, the sand sheet covers the underlying surface, and dune forms are found modifying the surface of the sand sheet. In this case transverse dunes are more common. Barchan dunes "point" down wind
Investigating Mars: Olympia Undae

NASA Image and Video Library

2018-03-09

This VIS image of Olympia Undae was collected during north polar spring. The crests of the dunes and other surfaces are light colored, indicative of a frost covering. The density of dunes and the alignments of the dune crests varies with location, controlled by the amount of available sand and the predominant winds over time. As the season changes into summertime, the dune crests will lose the frost and reveal the darker sand beneath. Olympia Undae is a vast dune field in the north polar region of Mars. It consists of a broad sand sea or erg that partly rings the north polar cap from about 120° to 240°E longitude and 78° to 83°N latitude. The dune field covers an area of approximately 470,000 km2 (bigger than California, smaller than Texas). Olympia Undae is the largest continuous dune field on Mars. Olympia Undae is not the only dune field near the north polar cap, several other smaller fields exist in the same latitude, but in other ranges of longitude, e.g. Abolos and Siton Undae. Barchan and transverse dune forms are the most common. In regions with limited available sand individual barchan dunes will form, the surface beneath and between the dunes is visible. In regions with large sand supplies, the sand sheet covers the underlying surface, and dune forms are found modifying the surface of the sand sheet. In this case transverse dunes are more common. Barchan dunes "point" down wind, transverse dunes are more linear and form parallel to the wind direction. The "square" shaped transverse dunes in Olympia Undae are due to two prevailing wind directions. The Odyssey spacecraft has spent over 15 years in orbit around Mars, circling the planet more than 71,000 times. It holds the record for longest working spacecraft at Mars. THEMIS, the IR/VIS camera system, has collected data for the entire mission and provides images covering all seasons and lighting conditions. Over the years many features of interest have received repeated imaging, building up a suite of
Investigating Mars: Olympia Undae

NASA Image and Video Library

2018-03-07

This VIS image of Olympia Undae was collected early in north polar spring. The crests of the dunes are light colored, indicative of a frost covering. As the season changes into summertime, the dune crests will lose the frost and reveal the darker sand beneath. The linear nature of transverse dunes can be seen at the bottom of the image. The density of dunes and the alignments of the dune crests varies with location, controlled by the amount of available sand and the predominant winds over time. Olympia Undae is a vast dune field in the north polar region of Mars. It consists of a broad sand sea or erg that partly rings the north polar cap from about 120° to 240°E longitude and 78° to 83°N latitude. The dune field covers an area of approximately 470,000 km2 (bigger than California, smaller than Texas). Olympia Undae is the largest continuous dune field on Mars. Olympia Undae is not the only dune field near the north polar cap, several other smaller fields exist in the same latitude, but in other ranges of longitude, e.g. Abolos and Siton Undae. Barchan and transverse dune forms are the most common. In regions with limited available sand individual barchan dunes will form, the surface beneath and between the dunes is visible. In regions with large sand supplies, the sand sheet covers the underlying surface, and dune forms are found modifying the surface of the sand sheet. In this case transverse dunes are more common. Barchan dunes "point" down wind, transverse dunes are more linear and form parallel to the wind direction. The "square" shaped transverse dunes in Olympia Undae are due to two prevailing wind directions. The Odyssey spacecraft has spent over 15 years in orbit around Mars, circling the planet more than 71,000 times. It holds the record for longest working spacecraft at Mars. THEMIS, the IR/VIS camera system, has collected data for the entire mission and provides images covering all seasons and lighting conditions. Over the years many features of
Investigating Mars: Olympia Undae

NASA Image and Video Library

2018-03-05

Olympia Undae is a vast dune field in the north polar region of Mars. It consists of a broad sand sea or erg that partly rings the north polar cap from about 120° to 240°E longitude and 78° to 83°N latitude. The dune field covers an area of approximately 470,000 km2 (bigger than California, smaller than Texas). Olympia Undae is the largest continuous dune field on Mars. Olympia Undae is not the only dune field near the north polar cap, several other smaller fields exist in the same latitude, but in other ranges of longitude, e.g. Abolos and Siton Undae. Barchan and transverse dune forms are the most common. In regions with limited available sand individual barchan dunes will form, the surface beneath and between the dunes is visible. In regions with large sand supplies, the sand sheet covers the underlying surface, and dune forms are found modifying the surface of the sand sheet. In this case transverse dunes are more common. Barchan dunes "point" down wind, transverse dunes are more linear and form parallel to the wind direction. The "square" shaped transverse dunes in Olympia Undae are due to two prevailing wind directions. The Odyssey spacecraft has spent over 15 years in orbit around Mars, circling the planet more than 71,000 times. It holds the record for longest working spacecraft at Mars. THEMIS, the IR/VIS camera system, has collected data for the entire mission and provides images covering all seasons and lighting conditions. Over the years many features of interest have received repeated imaging, building up a suite of images covering the entire feature. From the deepest chasma to the tallest volcano, individual dunes inside craters and dune fields that encircle the north pole, channels carved by water and lava, and a variety of other feature, THEMIS has imaged them all. For the next several months the image of the day will focus on the Tharsis volcanoes, the various chasmata of Valles Marineris, and the major dunes fields. We hope you enjoy these
The Developmental Education Program at Tacoma Community College.

ERIC Educational Resources Information Center

Spangler, Richard C.; Simonson, Carolyn

Tacoma Community College (TCC) is a comprehensive institution serving 7,000 students per quarter out of the 200,000 residents of Tacoma-Pierce County. Statistics show that 20% of the student population have not graduated from high school; 21% are from minority groups; and an estimated 60% are academically, educationally, or socially disadvantaged.…
Investigating Mars: Olympia Undae

NASA Image and Video Library

2018-03-08

This VIS image of Olympia Undae was collected during north polar spring. The crests of the dunes and other surfaces are light colored, indicative of a frost covering. At the top right of the image is a region of smooth surfaces. This is the ejecta from Jojutla Crater. The ejecta is a higher elevation than the rest of the surface, and dunes are "climbing" or "skirting" the ejecta regions. The density of dunes and the alignments of the dune crests varies with location, controlled by the amount of available sand and the predominant winds over time, and, in this case, the presence of different surface elevations. As the season changes into summertime, the dune crests will lose the frost and reveal the darker sand beneath. This loss of frost is just starting to be visible at the bottom of the image. Olympia Undae is a vast dune field in the north polar region of Mars. It consists of a broad sand sea or erg that partly rings the north polar cap from about 120° to 240°E longitude and 78° to 83°N latitude. The dune field covers an area of approximately 470,000 km2 (bigger than California, smaller than Texas). Olympia Undae is the largest continuous dune field on Mars. Olympia Undae is not the only dune field near the north polar cap, several other smaller fields exist in the same latitude, but in other ranges of longitude, e.g. Abolos and Siton Undae. Barchan and transverse dune forms are the most common. In regions with limited available sand individual barchan dunes will form, the surface beneath and between the dunes is visible. In regions with large sand supplies, the sand sheet covers the underlying surface, and dune forms are found modifying the surface of the sand sheet. In this case transverse dunes are more common. Barchan dunes "point" down wind, transverse dunes are more linear and form parallel to the wind direction. The "square" shaped transverse dunes in Olympia Undae are due to two prevailing wind directions. The Odyssey spacecraft has spent over 15

75 FR 53572 - Safety Zone; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-01

...-AA00 Safety Zone; Olympia Harbor Days Tug Boat Races, Budd Inlet, WA AGENCY: Coast Guard, DHS. ACTION... channel in Budd Inlet, WA during Olympia Harbor Days tug boat races. This safety zone is necessary to... waters of the Budd Inlet, WA during Olympia Harbor Days tug boat races. DATES: This rule is effective...
Investigating Mars: Olympia Undae

NASA Image and Video Library

2018-03-16

This VIS image was collected at the height of summer. It is during this season that winds are able to move sand sized particles, slowly modifying the dunes. Olympia Undae is a vast dune field in the north polar region of Mars. It consists of a broad sand sea or erg that partly rings the north polar cap from about 120° to 240°E longitude and 78° to 83°N latitude. The dune field covers an area of approximately 470,000 km2 (bigger than California, smaller than Texas). Olympia Undae is the largest continuous dune field on Mars. Olympia Undae is not the only dune field near the north polar cap, several other smaller fields exist in the same latitude, but in other ranges of longitude, e.g. Abolos and Siton Undae. Barchan and transverse dune forms are the most common. In regions with limited available sand individual barchan dunes will form, the surface beneath and between the dunes is visible. In regions with large sand supplies, the sand sheet covers the underlying surface, and dune forms are found modifying the surface of the sand sheet. In this case transverse dunes are more common. Barchan dunes "point" down wind, transverse dunes are more linear and form parallel to the wind direction. The "square" shaped transverse dunes in Olympia Undae are due to two prevailing wind directions. The density of dunes and the alignments of the dune crests varies with location, controlled by the amount of available sand and the predominant winds over time. The Odyssey spacecraft has spent over 15 years in orbit around Mars, circling the planet more than 71,000 times. It holds the record for longest working spacecraft at Mars. THEMIS, the IR/VIS camera system, has collected data for the entire mission and provides images covering all seasons and lighting conditions. Over the years many features of interest have received repeated imaging, building up a suite of images covering the entire feature. From the deepest chasma to the tallest volcano, individual dunes inside craters and
77 FR 43513 - Olympia Harbor Days Tug Boat Races, Budd Inlet, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-25

... Harbor Days Tug Boat Races, Budd Inlet, WA AGENCY: Coast Guard, DHS. ACTION: Notice of enforcement of regulation. SUMMARY: The Coast Guard will enforce the Special Local Regulation, Olympia Harbor Days Tug Boat... Special Local Regulation for Olympia Harbor Days Tug Boat Races, Budd Inlet, WA in 33 CFR 100.1309 on...
Investigating Mars: Olympia Undae

NASA Image and Video Library

2018-03-15

This VIS image highlights the dune form/dune density aspects of Olypmia Undae. In the center there is a brighter, diagonal region of few dunes. These dunes are the arc or crescent shape of barchan dunes. As more sand becomes available the barchan dunes begin to merge into transverse dunes. The region of dunes surrounding the bright swath still have the underlying surface visible, and the transverse dunes have a lace-like layout. In the regions with a significant abundance of sand have developed the tightly packed transverse dunes with the wave-like distribution. Olympia Undae is a vast dune field in the north polar region of Mars. It consists of a broad sand sea or erg that partly rings the north polar cap from about 120° to 240°E longitude and 78° to 83°N latitude. The dune field covers an area of approximately 470,000 km2 (bigger than California, smaller than Texas). Olympia Undae is the largest continuous dune field on Mars. Olympia Undae is not the only dune field near the north polar cap, several other smaller fields exist in the same latitude, but in other ranges of longitude, e.g. Abolos and Siton Undae. Barchan and transverse dune forms are the most common. In regions with limited available sand individual barchan dunes will form, the surface beneath and between the dunes is visible. In regions with large sand supplies, the sand sheet covers the underlying surface, and dune forms are found modifying the surface of the sand sheet. In this case transverse dunes are more common. Barchan dunes "point" down wind, transverse dunes are more linear and form parallel to the wind direction. The "square" shaped transverse dunes in Olympia Undae are due to two prevailing wind directions. The density of dunes and the alignments of the dune crests varies with location, controlled by the amount of available sand and the predominant winds over time. The Odyssey spacecraft has spent over 15 years in orbit around Mars, circling the planet more than 71,000 times. It holds
RadNet Air Data From Olympia, WA

EPA Pesticide Factsheets

This page presents radiation air monitoring and air filter analysis data for Olympia, WA from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.
Species profiles: Life histories and environmental requirements of coastal fishes and invertebrates (Pacific Northwest): Olympia oyster

DOE Office of Scientific and Technical Information (OSTI.GOV)

Couch, D.; Hassler, T.J.

Species profiles are literature summaries of the taxonomy, morphology, range, life history, and environmental requirements of coastal aquatic species. They are designed to assist in environmental impact assessments. Olympia oysters initially spawn as males then alternate their functional genders. Spawning begins at 13-16 {degree}C and occurs from spring to fall. After a short planktonic stage, larvae attach to the substrate. Olympia oysters thrive at salinities of 25 ppt or above; they are killed by freezing temperatures. Olympia oysters once supported large sustenance and commercial fisheries. Olympia oysters have not returned to pre-exploitation population levels which declined because of pollution andmore » loss of habitat. 32 refs., 2 figs.« less
Changing Dunes and Ripples in Olympia Undae

NASA Image and Video Library

2014-04-30

Olympia Undae is a large field of sand dunes surrounding Mars North Polar ice cap. High latitude covers them with water and carbon dioxide frost in the winter so they are illuminated. NASA Mars Reconnaissance Orbiter views these best in summer.
Pathfinding the Flight Advanced Stirling Convertor Design with the ASC-E3

NASA Technical Reports Server (NTRS)

Wong, Wayne A.; Wilson, Kyle; Smith, Eddie; Collins, Josh

2012-01-01

The Advanced Stirling Convertor (ASC) was initially developed by Sunpower, Inc. under contract to NASA Glenn Research Center (GRC) as a technology development project. The ASC technology fulfills NASA's need for high efficiency power convertors for future Radioisotope Power Systems (RPS). Early successful technology demonstrations between 2003 to 2005 eventually led to the expansion of the project including the decision in 2006 to use the ASC technology on the Advanced Stirling Radioisotope Generator (ASRG). Sunpower has delivered 22 ASC convertors of progressively mature designs to date to GRC. Currently, Sunpower with support from GRC, Lockheed Martin Space System Company (LMSSC), and the Department of Energy (DOE) is developing the flight ASC-F in parallel with the ASC-E3 pathfinders. Sunpower will deliver four pairs of ASC-E3 convertors to GRC which will be used for extended operation reliability assessment, independent validation and verification testing, system interaction tests, and to support LMSSC controller verification. The ASC-E3 and -F convertors are being built to the same design and processing documentation and the same product specification. The initial two pairs of ASC-E3 are built before the flight units and will validate design and processing changes prior to implementation on the ASC-F flight convertors. This paper provides a summary on development of the ASC technology and the status of the ASC-E3 build and how they serve the vital pathfinder role ahead of the flight build for ASRG. The ASRG is part of two of the three candidate missions being considered for selection for the Discovery 12 mission.
Zipcar Theater: The Tacoma Theater Project as an Anchor for Audience Development

ERIC Educational Resources Information Center

Kula, Michael

2015-01-01

As theater audiences decline society-wide, university theater programs have felt the impact, with many being cut back as institutions face difficult economic decisions. The Tacoma Theater Project at the University of Washington-Tacoma is an innovative effort to resist this trend. By working with professional theater groups in a partnership…
Geologic Map of the Olympia Cavi Region of Mars (MTM 85200): A Summary of Tactical Approaches

NASA Technical Reports Server (NTRS)

Skinner, J. A., Jr.; Herkenhoff, K.

2010-01-01

The 1:500K-scale geologic map of MTM 85200 - the Olympia Cavi region of Mars - has been submitted for peer review [1]. Physiographically, the quadrangle includes portions of Olympia Rupes, a set of sinuous scarps which elevate Planum Boreum 800 meters above Olympia Planum. The region includes the high-standing, spiral troughs of Boreales Scopuli, the rugged and deep depressions of Olympia Cavi, and the vast dune fields of Olympia Undae. Geologically, the mapped units and landforms reflect the recent history of repeated accumulation and degradation. The widespread occurrence of both weakly and strongly stratified units implicates the drape-like accumulation of ice, dust, and sand through climatic variations. Similarly, the occurrence of layer truncations, particularly at unit boundaries, implicates punctuated periods of both localized and regional erosion and surface deflation whereby underlying units were exhumed and their material transported and re-deposited. Herein, we focus on the iterative mapping approaches that allowed not only the accommodation of the burgeoning variety and volume of data sets, but also facilitated the efficient presentation of map information. Unit characteristics and their geologic history are detailed in past abstracts [2-3].
76 FR 38011 - Safety Zone; Hylebos Bridge Restoration, Hylebos Waterway, Tacoma, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-29

...-AA00 Safety Zone; Hylebos Bridge Restoration, Hylebos Waterway, Tacoma, WA AGENCY: Coast Guard, DHS... extending 50 yards to the north and south of the Hylebos Bridge, Tacoma, WA in both directions along the entire length of the Hylebos Bridge to ensure the safety of the boating public during the Hylebos Bridge...
77 FR 25168 - Appraisal Subcommittee (ASC); ASC Rules of Operation; Amended

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-27

... heads of the Bureau of Consumer Financial Protection and the Federal Housing Finance Agency. The ASC Rules of Operation serve as corporate bylaws outlining the ASC's purpose, functions, authority... Title XI. The ASC Rules of Operation serve as corporate bylaws outlining the ASC's purpose, functions...
12 CFR 1102.302 - ASC authority and functions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 7 2010-01-01 2010-01-01 false ASC authority and functions. 1102.302 Section 1102.302 Banks and Banking FEDERAL FINANCIAL INSTITUTIONS EXAMINATION COUNCIL APPRAISER REGULATION Description of Office, Procedures, Public Information § 1102.302 ASC authority and functions. (a) Authority...
76 FR 14829 - Safety Zone; 2011 Hylebos Bridge Restoration, Hylebos Waterway, Tacoma, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-18

...-AA00 Safety Zone; 2011 Hylebos Bridge Restoration, Hylebos Waterway, Tacoma, WA AGENCY: Coast Guard... temporary safety zone extending 50 yards to the north and south of the Hylebos Bridge, Tacoma, WA in both directions along the entire length of the Hylebos Bridge to ensure the safety of the boating public during...
Performance Measurement of Advanced Stirling Convertors (ASC-E3)

NASA Technical Reports Server (NTRS)

Oriti, Salvatore M.

2013-01-01

NASA Glenn Research Center (GRC) has been supporting development of the Advanced Stirling Radioisotope Generator (ASRG) since 2006. A key element of the ASRG project is providing life, reliability, and performance testing data of the Advanced Stirling Convertor (ASC). The latest version of the ASC (ASC-E3, to represent the third cycle of engineering model test hardware) is of a design identical to the forthcoming flight convertors. For this generation of hardware, a joint Sunpower and GRC effort was initiated to improve and standardize the test support hardware. After this effort was completed, the first pair of ASC-E3 units was produced by Sunpower and then delivered to GRC in December 2012. GRC has begun operation of these units. This process included performance verification, which examined the data from various tests to validate the convertor performance to the product specification. Other tests included detailed performance mapping that encompassed the wide range of operating conditions that will exist during a mission. These convertors were then transferred to Lockheed Martin for controller checkout testing. The results of this latest convertor performance verification activity are summarized here.
77 FR 58366 - Tacoma Power; Errata Notice

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-20

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Project No. 460-066] Tacoma Power; Errata Notice On September 11, 2012, the Commission issued a Notice of Application for Amendment of License and Soliciting Comments, Motions to Intervene, and Protests for the Cushman Hydroelectric Project...
77 FR 64838 - Notice of Intent To Rule on Request To Release Airport Property at the Seattle-Tacoma...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-23

... To Release Airport Property at the Seattle-Tacoma International Airport, Seattle, Washington AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of request to release airport property. SUMMARY... International Airport under the provisions of Section 125 of the Wendell H. Ford Aviation Investment Reform Act...
78 FR 15112 - Notice of Intent To Rule on Request To Release Airport Property at the Seattle-Tacoma...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-08

... To Release Airport Property at the Seattle-Tacoma International Airport, Seattle, Washington AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of Request to Release Airport Property. SUMMARY... International Airport under the provisions of Section 125 of the Wendell H. Ford Aviation Investment Reform Act...
Advanced Stirling Convertor (ASC-E2) Performance Testing at NASA Glenn Research Center

NASA Technical Reports Server (NTRS)

Oriti, Salvatore; Wilson, Scott

2011-01-01

The National Aeronautics and Space Administration (NASA) Glenn Research Center (GRC) has been supporting development of the Advanced Stirling Radioisotope Generator (ASRG) since 2006. A key element of the ASRG Project is providing life, reliability, and performance testing of the Advanced Stirling Convertor (ASC). For this purpose, four pairs of ASCs capable of operating to 850 C and designated with the model number ASC-E2, were delivered by Sunpower of Athens, OH, to GRC in 2010. The ASC-E2s underwent a series of tests that included workmanship vibration testing, performance mapping, and extended operation. Workmanship vibration testing was performed following fabrication of each convertor to verify proper hardware build. Performance mapping consisted of operating each convertor at various conditions representing the range expected during a mission. Included were conditions representing beginning-of-mission (BOM), end-of-mission (EOM), and fueling. This same series of tests was performed by Sunpower prior to ASC-E2 delivery. The data generated during the GRC test were compared to performance before delivery. Extended operation consisted of a 500-hour period of operation with conditions maintained at the BOM point. This was performed to demonstrate steady convertor performance following performance mapping. Following this initial 500-hour period, the ASC-E2s will continue extended operation, controller development and special durability testing, during which the goal is to accumulate tens of thousands of hours of operation. Data collected during extended operation will support reliability analysis. Performance data from these tests is summarized in this paper.
Advanced Stirling Convertor (ASC-E2) Performance Testing at NASA Glenn Research Center

NASA Technical Reports Server (NTRS)

Oriti, Salvatore; Wilson, Scott

2011-01-01

The National Aeronautics and Space Administration (NASA) Glenn Research Center (GRC) has been supporting development of the Advanced Stirling Radioisotope Generator (ASRG) since 2006. A key element of the ASRG Project is providing life, reliability, and performance testing of the Advanced Stirling Convertor (ASC). For this purpose, four pairs of ASCs capable of operating to 850 C and designated with the model number ASC-E2, were delivered by Sunpower of Athens, Ohio, to GRC in 2010. The ASC-E2s underwent a series of tests that included workmanship vibration testing, performance mapping, and extended operation. Workmanship vibration testing was performed following fabrication of each convertor to verify proper hardware build. Performance mapping consisted of operating each convertor at various conditions representing the range expected during a mission. Included were conditions representing beginning-of-mission (BOM), end-of-mission (EOM), and fueling. This same series of tests was performed by Sunpower prior to ASC-E2 delivery. The data generated during the GRC test were compared to performance before delivery. Extended operation consisted of a 500-hr period of operation with conditions maintained at the BOM point. This was performed to demonstrate steady convertor performance following performance mapping. Following this initial 500-hr period, the ASC-E2s will continue extended operation, controller development and special durability testing, during which the goal is to accumulate tens of thousands of hours of operation. Data collected during extended operation will support reliability analysis. Performance data from these tests is summarized in this paper.

Remediation System Evaluation, Former Occidental Facility in Tacoma, Washington

EPA Pesticide Factsheets

The former Occidental Chemical Corporation (OCC) property (“site”) is approximately 37 acres in extent and is located at 605 Alexander Avenue in Tacoma, Washington along the Hylebos Waterway. This is a Remediation System Evaluation document.
Advanced Stirling Convertor (ASC) Technology Maturation

NASA Technical Reports Server (NTRS)

Wong, Wayne A.; Wilson, Scott; Collins, Josh; Wilson, Kyle

2015-01-01

The Advanced Stirling Convertor (ASC) development effort was initiated by NASA Glenn Research Center (GRC) with contractor Sunpower Inc. to develop high efficiency thermal-to-electric power conversion technology for NASA Radioisotope Power Systems. Early successful performance demonstrations led to the expansion of the project as well as adoption of the technology by the Department of Energy (DOE) and system integration contractor Lockheed Martin Space Systems Company as part of the Advanced Stirling Radioisotope Generator (ASRG) flight project. The ASRG integrates a pair of ASCs to convert the heat from a pair of General Purpose Heat Source (GPHS) modules into electrical power. The expanded NASA ASC effort included development of several generations of ASC prototypes or Engineering Units to help prepare the ASC technology and Sunpower for flight implementation. Sunpower later had two parallel contracts allowing the last of the NASA Engineering Units called ASC-E3 to serve as pathfinders for the ASC-F flight convertors being built for DOE. The ASC-E3 convertors utilized the ASC-F flight specifications and were built using the ASC-F design and process documentation. Shortly after the first ASC-F Pair achieved initial operation, due to budget constraints, the DOE ASRG flight development contract was terminated. NASA continues to invest in the development of Stirling RPS technology including continued production of the ASC-E3 convertors, seven of which have been delivered with one additional unit in production. Starting in FY2015, Stirling Convertor Technology Maturation has been reorganized as an element of the RPS Stirling Cycle Technology Development (SCTD) Project and long-term plans for continued Stirling technology advancement are in reformulation. This paper provides a status on the ASC project, an overview of advancements made in the design and production of the ASC at Sunpower, and a summary of acceptance tests, reliability tests, and tactical tests at NASA
Advanced Stirling Convertor (ASC) Technology Maturation

NASA Technical Reports Server (NTRS)

Wong, Wayne A.; Wilson, Scott; Collins, Josh; Wilson, Kyle

2016-01-01

The Advanced Stirling Convertor (ASC) development effort was initiated by NASA Glenn Research Center with contractor Sunpower, Inc., to develop high-efficiency thermal-to-electric power conversion technology for NASA Radioisotope Power Systems (RPSs). Early successful performance demonstrations led to the expansion of the project as well as adoption of the technology by the Department of Energy (DOE) and system integration contractor Lockheed Martin Space Systems Company as part of the Advanced Stirling Radioisotope Generator (ASRG) flight project. The ASRG integrates a pair of ASCs to convert the heat from a pair of General Purpose Heat Source (GPHS) modules into electrical power. The expanded NASA ASC effort included development of several generations of ASC prototypes or engineering units to help prepare the ASC technology and Sunpower for flight implementation. Sunpower later had two parallel contracts allowing the last of the NASA engineering units called ASC-E3 to serve as pathfinders for the ASC-F flight convertors being built for DOE. The ASC-E3 convertors utilized the ASC-F flight specifications and were built using the ASC-F design and process documentation. Shortly after the first ASC-F pair achieved initial operation, due to budget constraints, the DOE ASRG flight development contract was terminated. NASA continues to invest in the development of Stirling RPS technology including continued production of the ASC-E3 convertors, seven of which have been delivered with one additional unit in production. Starting in fiscal year 2015, Stirling Convertor Technology Maturation has been reorganized as an element of the RPS Stirling Cycle Technology Development (SCTD) Project and long-term plans for continued Stirling technology advancement are in reformulation. This paper provides a status on the ASC project, an overview of advancements made in the design and production of the ASC at Sunpower, and a summary of acceptance tests, reliability tests, and tactical
Geologic map of the MTM 85200 quadrangle, Olympia Rupes region of Mars

USGS Publications Warehouse

Skinner, James A.; Herkenhoff, Kenneth E.

2012-01-01

The north polar region of Mars is dominated by Planum Boreum, a roughly circular, domical plateau that rises >2,500 m above the surrounding lowland. Planum Boreum is >1,500 km in diameter, contains deep, curvilinear troughs and chasmata, isolated cavi, and marginal scarps and slopes. The north polar plateau is surrounded by low-lying and nearly horizontal plains of various surface texture, geologic origin, and stratigraphic significance. The MTM 85200 quadrangle spans 5° of latitude (lat 82.5° to 87.5° N.) and 40° of longitude (long 140° to 180° E.) within the eastern hemisphere of Mars. The quadrangle includes the high-standing Planum Boreum, curvilinear troughs of Boreales Scopuli, deep, sinuous scarps of Olympia Rupes, isolated and coalesced depressions of Olympia Cavi, margins of the circular polar erg Olympia Undae, and low-standing Olympia Planum. The surface of Planum Boreum within the MTM 85200 quadrangle is characterized by smoothly sculptured landforms with shallow slopes and variable relief at kilometer scales. Areas that are perennially covered with bright frost are generally smooth and planar at 100-m scales. However, MGS MOC and MRO HiRISE images show that much of the icy polar plateau is rough at decameter scale. The Martian polar plateaus are likely to contain a record of global climate history for >107 to as much as ~3 x 109 years. This record is partly observable as rhythmically layered deposits exposed in the curvilinear troughs of the north polar plateau, Planum Boreum. The north polar layered deposits are widely interpreted to be among the most youthful bedrock deposits on the Martian surface. These materials and their stratigraphic and structural relations provide a glimpse into some of the more recent geologic processes that have occurred on Mars. The ability of the massive polar deposits to periodically trap and release both volatiles and lithic particles may represent a globally important, recurring geologic process for Mars.
76 FR 72839 - Drawbridge Operation Regulation; City Waterway Also Known as Thea Foss Waterway, Tacoma, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-28

... Street (``Murray Morgan'') Bridge across City Waterway also known as the Thea Foss Waterway, mile 0.6, at... INFORMATION: The City of Tacoma has requested to place the South 11th Street ``Murray Morgan'' Bridge in the... rehabilitation project on the bridge. The South 11th Street Bridge crosses City Waterway mile 0.6 at Tacoma, WA...
Expansion joint noise reduction on the new Tacoma Narrows Bridge.

DOT National Transportation Integrated Search

2011-12-01

"Washington State Department of Transportation (WSDOT) responded to citizen complaints about expansion joint noise on the new Tacoma Narrows Bridge (TNB) by installing new wall coated in noise absorptive materials. The goal of the project was to redu...
ASC Small Business Office

DTIC Science & Technology

2006-05-23

Aeronautical Systems Center Dominant Air Power: Design For Tomorrow…Deliver Today ASC Small Business Office 23 May 2006 Teresa Rendon ASC/BC...1. REPORT DATE 23 MAY 2006 2. REPORT TYPE 3. DATES COVERED 00-00-2006 to 00-00-2006 4. TITLE AND SUBTITLE ASC Small Business Office 5a...Limitation on Subcontracting • Teaming • Source Selection of Small Business Participation • Role of the Small Business Office • Questions 3 Dominant Air
Flood impact assessment on the development of Archaia Olympia riparian area in Greece.

NASA Astrophysics Data System (ADS)

Pasaporti, Christina; Podimata, Marianthi; Yannopoulos, Panayotis

2013-04-01

A long list of articles in the literature examines several issues of flood risk management and applications of flood scenarios, taking into consideration the climate changes, as well as decision making tools in flood planning. The present study tries to highlight the conversation concerning flood impacts on the development rate of a riparian area. More specifically, Archaia (Ancient) Olympia watershed was selected as a case study area, since it is considered as a region of special interest and international significance. In addition, Alfeios River, which is the longest river of Peloponnisos Peninsula, passes through the plain of Archaia Olympia. Flood risk scenarios allow scientists and practitioners to understand the adverse effects of flooding on development activities such as farming, tourism etc. and infrastructures in the area such as road and railway networks, Flokas dam and the hydroelectric power plant, bridges, settlements and other properties. Flood risks cause adverse consequences on the region of Archaia Olympia (Ancient Olympic stadium) and Natura 2000 site area. Furthermore, SWOT analysis was used in order to quantify multicriteria and socio-economic characteristics of the study area. SWOT analysis, as a planning method, indicates the development perspective by identifying the strengths, weaknesses, threads and opportunities. Subsequent steps in the process of intergraded River Management Plan of Archaia Olympia could be derived from SWOT analysis. The recognition and analysis of hydro-geomorphological influences on riparian development activities can lead to the definition of hazardous and vulnerability zones and special warning equipment. The former information combined with the use of the spatial database for the catchment area of the River Alfeios, which aims to gather multiple watershed data, could serve in preparing the Management Plan of River Basin District 01 where Alfeios R. belongs. Greece has to fulfill the obligation of implementing River
Earthquake Hazards and Lifelines in the Interstate 5 Urban Corridor - Woodburn, Oregon, to Centralia, Washington

USGS Publications Warehouse

Barnett, E.A.; Weaver, C.S.; Meagher, K.L.; Haugerud, R.A.; Wang, Z.; Madin, I.P.; Wang, Y.; Wells, R.E.; Blakely, R.J.; Ballantyne, D.B.; Darienzo, M.

2009-01-01

The Interstate 5 highway (I-5) corridor, which stretches from Mexico to Canada, is both the main economic artery of the Pacific Northwest and home to the majority of Oregonians and Washingtonians. Accordingly, most regional utility and transportation systems have major components located within the I-5 corridor. For the purposes of this map, we refer to these essential systems as lifeline systems. The Pacific Northwest section of I-5, the I-5 urban corridor, extends from Eugene, Oregon, to the border of Canada. The population of this region is rapidly increasing with the bulk of growth and economic development centered in the cities of Eugene, Salem, and Portland, Oregon, and Olympia, Tacoma, Seattle, Everett, and Bellingham, Washington.
Earthquake Hazards and Lifelines in the Interstate 5 Urban Corridor - Cottage Grove to Woodburn, Oregon

USGS Publications Warehouse

Barnett, E.A.; Weaver, C.S.; Meagher, K.L.; Haugerud, R.A.; Wang, Z.; Madin, I.P.; Wang, Y.; Wells, R.E.; Blakely, R.J.; Ballantyne, D.B.; Darienzo, M.

2009-01-01

The Interstate 5 highway (I-5) corridor, which stretches from Mexico to Canada, is both the main economic artery of the Pacific Northwest and home to the majority of Oregonians and Washingtonians. Accordingly, most regional utility and transportation systems have major components located within the I-5 corridor. For the purposes of this map, we refer to these essential systems as lifeline systems. The Pacific Northwest section of I-5, the I-5 urban corridor, extends from Eugene, Oregon, to the border of Canada. The population of this region is rapidly increasing with the bulk of growth and economic development centered in the cities of Eugene, Salem, and Portland, Oregon, and Olympia, Tacoma, Seattle, Everett, and Bellingham, Washington.
The Tacoma Narrows Bridge Collapse on Film and Video

ERIC Educational Resources Information Center

Olson, Don; Hook, Joseph; Doescher, Russell; Wolf, Steven

2015-01-01

This month marks the 75th anniversary of the Tacoma Narrows Bridge collapse. During a gale on Nov. 7, 1940, the bridge exhibited remarkable oscillations before collapsing spectacularly (Figs. 1-5). Physicists over the years have spent a great deal of time and energy studying this event. By using open-source analysis tools and digitized footage of…
Advanced Stirling Convertor (ASC) Development for NASA RPS

NASA Technical Reports Server (NTRS)

Wong, Wayne A.; Wilson, Scott; Collins, Josh

2014-01-01

Sunpower's Advanced Stirling Convertor (ASC) initiated development under contract to the NASA Glenn Research Center (GRC) and after a series of successful demonstrations, the ASC began transitioning from a technology development project to flight development project. The ASC has very high power conversion efficiency making it attractive for future Radioisotope Power Systems (RPS) in order to make best use of the low plutonium-238 fuel inventory in the U.S. In recent years, the ASC became part of the NASA-Department of Energy Advanced Stirling Radioisotope Generator (ASRG) Integrated Project. Sunpower held two parallel contracts to produce ASC convertors, one with the Department of Energy/Lockheed Martin to produce the ASC-F flight convertors, and one with NASA GRC for the production of ASC-E3 engineering units, the initial units of which served as production pathfinders. The integrated ASC technical team successfully overcame various technical challenges that led to the completion and delivery of the first two pairs of flight-like ASC-E3 by 2013. However, in late Fall 2013, the DOE initiated termination of the Lockheed Martin ASRG flight development contract driven primarily by budget constraints. NASA continues to recognize the importance of high efficiency ASC power conversion for RPS and continues investment in the technology including the continuation of ASC-E3 production at Sunpower and the assembly of the ASRG Engineering Unit #2. This paper provides a summary of ASC technical accomplishments, overview of tests at GRC, plans for continued ASC production at Sunpower, and status of Stirling technology development.
How Do Social Service Providers View Recent Immigrants? Perspectives from Portland, Maine, and Olympia, Washington

PubMed Central

CLEVENGER, CASEY; DERR, AMELIA SERAPHIA; CADGE, WENDY; CURRAN, SARA

2014-01-01

This article explores how social service providers in two small, geographically distinct cities—Portland, Maine, and Olympia, Washington—understand the importance of welcoming and incorporating new immigrants in their cities. We focus on how providers characterize their responsibilities, how they understand the importance of responding to new immigrants, and what they describe as the challenges and opportunities presented by recent immigration to their cities. Despite differences in Portland and Olympia, we find that providers in both cities combine a sense of moral responsibility to help immigrants, with an emphasis on the economic and cultural resources immigrants bring to cities. These insights expand recent immigration scholarship from a focus on immigrants alone to include the perspectives and logics of social service workers who are often their first points of contact in new places. PMID:25110469
Lifelines and Earthquake Hazards in the Interstate 5 Urban Corridor: Cottage Grove to Woodburn, Oregon

USGS Publications Warehouse

Barnett, E.A.; Weaver, C.S.; Meagher, K.L.; Wang, Z.; Madin, I.P.; Wang, M.; Haugerud, R.A.; Wells, R.E.; Ballantyne, D.B.; Darienzo, M.; ,

2004-01-01

The Interstate 5 highway corridor, stretching from Mexico to Canada, is not only the economic artery of the Pacific Northwest, but is also home to the majority of Oregonians and Washingtonians. Accordingly, most regional utility and transportation systems, such as railroads and electrical transmission lines, have major components in the I-5 corridor. The section of I-5 from Cottage Grove, Oregon, to Blaine, Washington, is rapidly urbanizing, with population growth and economic development centered around the cities of Eugene, Salem, Portland, Olympia, Tacoma, Seattle, Everett, and Bellingham. For the purposes of this map, we refer to this area as the I-5 Urban Corridor. This publicaton consists of two large sheets: A map and a text-and-figures poster.
Remediation System Evaluation, Comm. Bay/South Tacoma Channel, Well 12A Superfund Site

EPA Pesticide Factsheets

The first operable unit (OU1) of the Commencement Bay/South Tacoma Channel Superfund Site addressessoil and groundwater contamination associated with the Time Oil property that was first discovered inpublic supply Well 12A in 1981.
77 FR 48535 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-14

... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-10844; 2200-1100-665] Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Washington State Parks and Recreation Commission has...
78 FR 13887 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-01

... kinship evidence provided at consultation, Washington State Parks staff also determined a cultural... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-12278; 2200-1100-665] Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA AGENCY: National Park...
Functional and Structural Characterization of Zebrafish ASC.

PubMed

Li, Yajuan; Huang, Yi; Cao, Xiaocong; Yin, Xueying; Jin, Xiangyu; Liu, Sheng; Jiang, Jiansheng; Jiang, Wei; Xiao, Tsan Sam; Zhou, Rongbin; Cai, Gang; Hu, Bing; Jin, Tengchuan

2018-05-23

The zebrafish genome encodes homologs for most of the proteins involved in inflammatory pathways; however, the molecular components and activation mechanisms of fish inflammasomes are largely unknown. ASC (apoptosis-associated speck-like protein containing a caspase-recruitment domain (CARD)) is the only adaptor involved in the formation of multiple types of inflammasomes. Here, we demonstrate that zASC is also involved in inflammasome activation in zebrafish. When overexpressed in vitro and in vivo in zebrafish, both the zASC and zASC pyrin domain (PYD) proteins form speck and filament structures. Importantly, the crystal structures of the N-terminal PYD and C-terminal CARD of zebrafish ASC were determined independently as two separate entities fused to maltose-binding protein (MBP). Structure-guided mutagenesis revealed the functional relevance of the PYD hydrophilic surface found in the crystal lattice. Finally, the fish caspase-1 homolog Caspy, but not the caspase-4/11 homolog Caspy2, interacts with zASC through homotypic PYD-PYD interactions, which differ from those in mammals. These observations establish the conserved and unique structural/functional features of the zASC-dependent inflammasome pathway. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
The collapse of Tacoma Narrows Bridge: a piece to the puzzle

NASA Astrophysics Data System (ADS)

Walther, J. H.; Christensen, D. S.; Malthe, M. G.; Roenne, M.; Spietz, H. J.; Larsen, A.; Larsen, S. V.

2017-11-01

On Nov. 7th 1940 the newly constructed Tacoma Narrows Bridge collapsed due to excessive torsional oscillations caused by the formation and shedding of large coherent vortices. The subsequent wind tunnel tests conducted on both section- and full bridge models concluded that the bridge should have collapsed at a wind speed corresponding to approximately half of the wind speed at the day of the collapse. This discrepancy questions our understanding of the phenomena responsible for the failure of the bridge. The present study aims at clarifying this ``mystery'' by considering historical records made available by the US coast guards, and by performing wind tunnel tests and detailed numerical flow simulations. Our findings indicate that the discrepancy is caused by an until now unnoticed yawed wind direction relative to the bridge, which was present at the day of the collapse. Danish Council for Independent Research Grant No. 4184-00349B.
78 FR 44594 - Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-24

... DEPARTMENT OF THE INTERIOR National Park Service [NPS-WASO-NAGPRA-13405; PPWOCRADN0-PCU00RP14.R50000] Notice of Inventory Completion: Washington State Parks and Recreation Commission, Olympia, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Washington State Parks and...

The Catfish Lake Scarp, Allyn, Washington preliminary field data and implications for earthquake hazards posed by the Tacoma Fault

USGS Publications Warehouse

Sherrod, Brian L.; Nelson, Alan R.; Kelsey, Harvey M.; Brocher, Thomas M.; Blakely, Richard J.; Weaver, Craig S.; Rountree, Nancy K.; Rhea, B. Susan; Jackson, Bernard S.

2004-01-01

The Tacoma fault bounds gravity and aeromagnetic anomalies for 50 km across central Puget lowland from Tacoma to western Kitsap County. Tomography implies at least 6 km of post-Eocene uplift to the north of the fault relative to basinal sedimentary rocks to the south. Coastlines north of the Tacoma fault rose about 1100 years ago during a large earthquake. Abrupt uplift up to several meters caused tidal flats at Lynch Cove, North Bay, and Burley Lagoon to turn into forested wetlands and freshwater marshes. South of the fault at Wollochet Bay, Douglas-fir forests sank into the intertidal zone and changed into saltmarsh. Liquefaction features found beneath the marsh at Burley Lagoon point to strong ground shaking at the time of uplift. Recent lidar maps of the area southwest of Allyn, Washington revealed a 4 km long scarp, or two closely spaced en-echelon scarps, which correspond closely to the Tacoma fault gravity and aeromagnetic anomalies. The scarp, named the Catfish Lake scarp, is north-side-up, trends east-west, and clearly displace striae left by a Vashon-age glacier. A trench across the scarp exposed evidence for postglacial folding and reverse slip. No organic material for radiocarbon dating was recovered from the trench. However, relationships in the trench suggest that the folding and faulting is postglacial in age.
Sandia National Laboratories Advanced Simulation and Computing (ASC) software quality plan : ASC software quality engineering practices Version 3.0.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Turgeon, Jennifer L.; Minana, Molly A.; Hackney, Patricia

2009-01-01

The purpose of the Sandia National Laboratories (SNL) Advanced Simulation and Computing (ASC) Software Quality Plan is to clearly identify the practices that are the basis for continually improving the quality of ASC software products. Quality is defined in the US Department of Energy/National Nuclear Security Agency (DOE/NNSA) Quality Criteria, Revision 10 (QC-1) as 'conformance to customer requirements and expectations'. This quality plan defines the SNL ASC Program software quality engineering (SQE) practices and provides a mapping of these practices to the SNL Corporate Process Requirement (CPR) 001.3.6; 'Corporate Software Engineering Excellence'. This plan also identifies ASC management's and themore » software project teams responsibilities in implementing the software quality practices and in assessing progress towards achieving their software quality goals. This SNL ASC Software Quality Plan establishes the signatories commitments to improving software products by applying cost-effective SQE practices. This plan enumerates the SQE practices that comprise the development of SNL ASC's software products and explains the project teams opportunities for tailoring and implementing the practices.« less
North Polar Gypsum Dunes in Olympia Undae

NASA Image and Video Library

2016-07-15

These sand dunes are a type of aeolian bedform and partly encircle the Martian North Pole in a region called Olympia Undae. Unlike most of the sand dunes on Mars that are made of the volcanic rock basalt, these are made of a type of sulfate mineral called gypsum. Whence the sand? Well, gypsum is a mineral that can often form from the evaporation of water that has sulfur and calcium dissolved in it. This sand was probably sourced from a northern region on Mars that used to be quite wet. The boxy gridding of the dunes indicates that the wind blows in multiple directions. Note: "Aeolian" means wind-blown and "bedform" means piles of sediment shaped by a flowing fluid (liquid or gas). http://photojournal.jpl.nasa.gov/catalog/PIA20743
Risk-Informed Mean Recurrence Intervals for Updated Wind Maps in ASCE 7-16.

PubMed

McAllister, Therese P; Wang, Naiyu; Ellingwood, Bruce R

2018-05-01

ASCE 7 is moving toward adopting load requirements that are consistent with risk-informed design goals characteristic of performance-based engineering (PBE). ASCE 7-10 provided wind maps that correspond to return periods of 300, 700, and 1,700 years for Risk Categories I, II, and combined III/IV, respectively. The risk targets for Risk Categories III and IV buildings and other structures (designated as essential facilities) are different in PBE. The reliability analyses reported in this paper were conducted using updated wind load data to (1) confirm that the return periods already in ASCE 7-10 were also appropriate for risk-informed PBE, and (2) to determine a new risk-based return period for Risk Category IV. The use of data for wind directionality factor, K d , which has become available from recent wind tunnel tests, revealed that reliabilities associated with wind load combinations for Risk Category II structures are, in fact, consistent with the reliabilities associated with the ASCE 7 gravity load combinations. This paper shows that the new wind maps in ASCE 7-16, which are based on return periods of 300, 700, 1,700, and 3,000 years for Risk Categories I, II, III, and IV, respectively), achieve the reliability targets in Section 1.3.1.3 of ASCE 7-16 for nonhurricane wind loads.
42 CFR 416.125 - ASC facility services payment rate.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 3 2011-10-01 2011-10-01 false ASC facility services payment rate. 416.125 Section 416.125 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN... connection with the performance of that procedure. (b) The payment must be substantially less than would have...
42 CFR 416.125 - ASC facility services payment rate.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 3 2010-10-01 2010-10-01 false ASC facility services payment rate. 416.125 Section 416.125 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN... connection with the performance of that procedure. (b) The payment must be substantially less than would have...
Lateral Load Testing of the Advanced Stirling Convertor (ASC-E2) Heater Head

NASA Technical Reports Server (NTRS)

Cornell, Peggy A.; Krause, David L.; Davis, Glen; Robbie, Malcolm G.; Gubics, David A.

2010-01-01

Free-piston Stirling convertors are fundamental to the development of NASA s next generation of radioisotope power system, the Advanced Stirling Radioisotope Generator (ASRG). The ASRG will use General Purpose Heat Source (GPHS) modules as the energy source and Advanced Stirling Convertors (ASCs) to convert heat into electrical energy, and is being developed by Lockheed Martin under contract to the Department of Energy. Achieving flight status mandates that the ASCs satisfy design as well as flight requirements to ensure reliable operation during launch. To meet these launch requirements, GRC performed a series of quasi-static mechanical tests simulating the pressure, thermal, and external loading conditions that will be experienced by an ASC-E2 heater head assembly. These mechanical tests were collectively referred to as "lateral load tests" since a primary external load lateral to the heater head longitudinal axis was applied in combination with the other loading conditions. The heater head was subjected to the operational pressure, axial mounting force, thermal conditions, and axial and lateral launch vehicle acceleration loadings. To permit reliable prediction of the heater head s structural performance, GRC completed Finite Element Analysis (FEA) computer modeling for the stress, strain, and deformation that will result during launch. The heater head lateral load test directly supported evaluation of the analysis and validation of the design to meet launch requirements. This paper provides an overview of each element within the test and presents assessment of the modeling as well as experimental results of this task.
Lateral Load Testing of the Advanced Stirling Convertor (ASC-E2) Heater Head

NASA Technical Reports Server (NTRS)

Cornell, Peggy A.; Krause, David L.; Davis, Glen; Robbie, Malcolm G.; Gubics, David A.

2011-01-01

Free-piston Stirling convertors are fundamental to the development of NASA s next generation of radioisotope power system, the Advanced Stirling Radioisotope Generator (ASRG). The ASRG will use General Purpose Heat Source (GPHS) modules as the energy source and Advanced Stirling Convertors (ASCs) to convert heat into electrical energy, and is being developed by Lockheed Martin under contract to the Department of Energy. Achieving flight status mandates that the ASCs satisfy design as well as flight requirements to ensure reliable operation during launch. To meet these launch requirements, GRC performed a series of quasi-static mechanical tests simulating the pressure, thermal, and external loading conditions that will be experienced by an ASC-E2 heater head assembly. These mechanical tests were collectively referred to as "lateral load tests" since a primary external load lateral to the heater head longitudinal axis was applied in combination with the other loading conditions. The heater head was subjected to the operational pressure, axial mounting force, thermal conditions, and axial and lateral launch vehicle acceleration loadings. To permit reliable prediction of the heater head s structural performance, GRC completed Finite Element Analysis (FEA) computer modeling for the stress, strain, and deformation that will result during launch. The heater head lateral load test directly supported evaluation of the analysis and validation of the design to meet launch requirements. This paper provides an overview of each element within the test and presents assessment of the modeling as well as experimental results of this task.
Dynamics of in vivo ASC speck formation

PubMed Central

2017-01-01

Activated danger or pathogen sensors trigger assembly of the inflammasome adaptor ASC into specks, large signaling platforms considered hallmarks of inflammasome activation. Because a lack of in vivo tools has prevented the study of endogenous ASC dynamics, we generated a live ASC reporter through CRISPR/Cas9 tagging of the endogenous gene in zebrafish. We see strong ASC expression in the skin and other epithelia that act as barriers to insult. A toxic stimulus triggered speck formation and rapid pyroptosis in keratinocytes in vivo. Macrophages engulfed and digested that speck-containing, pyroptotic debris. A three-dimensional, ultrastructural reconstruction, based on correlative light and electron microscopy of the in vivo assembled specks revealed a compact network of highly intercrossed filaments, whereas pyrin domain (PYD) or caspase activation and recruitment domain alone formed filamentous aggregates. The effector caspase is recruited through PYD, whose overexpression induced pyroptosis but only after substantial delay. Therefore, formation of a single, compact speck and rapid cell-death induction in vivo requires a full-length ASC. PMID:28701426
Sandia National Laboratories Advanced Simulation and Computing (ASC) software quality plan. Part 1: ASC software quality engineering practices, Version 2.0.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sturtevant, Judith E.; Heaphy, Robert; Hodges, Ann Louise

2006-09-01

The purpose of the Sandia National Laboratories Advanced Simulation and Computing (ASC) Software Quality Plan is to clearly identify the practices that are the basis for continually improving the quality of ASC software products. The plan defines the ASC program software quality practices and provides mappings of these practices to Sandia Corporate Requirements CPR 1.3.2 and 1.3.6 and to a Department of Energy document, ASCI Software Quality Engineering: Goals, Principles, and Guidelines. This document also identifies ASC management and software project teams responsibilities in implementing the software quality practices and in assessing progress towards achieving their software quality goals.
Advanced Stirling Convertor (ASC) Technology Maturation in Preparation for Flight

NASA Technical Reports Server (NTRS)

Wong, Wayne A.; Cornell, Peggy A.

2012-01-01

The Advanced Stirling Convertor (ASC) is being developed by an integrated team of Sunpower and National Aeronautics and Space Administration s (NASA s) Glenn Research Center (GRC). The ASC development, funded by NASA s Science Mission Directorate, started as a technology development effort in 2003 and has since evolved through progressive convertor builds and successful testing to demonstrate high conversion efficiency, low mass, and capability to meet long-life Radioisotope Power System (RPS) requirements. The technology has been adopted by the Department of Energy and Lockheed Martin Space Systems Company s Advanced Stirling Radioisotope Generator (ASRG), which has been selected for potential flight demonstration on Discovery 12. This paper provides an overview of the status of ASC development including the most recent ASC-E2 convertors that have been delivered to GRC and an introduction to the ASC-E3 and ASC flight convertors that Sunpower will build next. The paper also describes the technology maturation and support tasks being conducted at GRC to support ASC and ASRG development in the areas of convertor and generator extended operation, high-temperature materials, heater head life assessment, organics, nondestructive inspection, spring fatigue testing, and other reliability verification tasks.
Adipose-derived stem cell (ASC)-enriched fat grafting: experiments using White rabbits and an automated cell processing apparatus.

PubMed

Kakudo, Natsuko; Morimoto, Naoki; Ogawa, Takeshi; Hihara, Masakatsu; Lai, Fangyuan; Kusumoto, Kenji

2017-09-01

The grafting of fat mixed with adipose-derived stem cells (ASCs) is being increasingly applied to compensate for the disadvantages of previous fat grafting methods. Devices that automatically isolate fat stem cells also have recently been developed. ASCs were isolated from the inguinal region of White rabbits using Icellator ® , and the number of cells and their viability were measured. The cell count per fat graft (mL) was adjusted to the following concentrations and subcutaneously transplanted into the back: Control group, Fat + PBS; Fat + ASCs (×0.5) group, 1.6 × 10 5 cells/mL; and Fat + ASCs (×1) group, 3.2 × 10 5 cells/mL. Grafted fat weight was measured after 8 weeks, and histological, immunohistological, and specifically stained sections were prepared. Fat absorption was reduced in Fat + ASCs (×0.5) and Fat + ASCs (×1) groups. The number of blood vessels was higher in Fat + ASCs (×1) than in the control group, and blood vessel areas were higher in Fat + ASCs (×0.5) and Fat + ASCs (×1) groups than in the control group. The usefulness of the automated cell processing apparatus, Icellator ® , was confirmed, and the results obtained suggest that grafted ASCs promote the vascularization and engraftment of fat grafts.
Sandia National Laboratories Advanced Simulation and Computing (ASC) software quality plan. Part 1 : ASC software quality engineering practices version 1.0.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Minana, Molly A.; Sturtevant, Judith E.; Heaphy, Robert

2005-01-01

The purpose of the Sandia National Laboratories (SNL) Advanced Simulation and Computing (ASC) Software Quality Plan is to clearly identify the practices that are the basis for continually improving the quality of ASC software products. Quality is defined in DOE/AL Quality Criteria (QC-1) as conformance to customer requirements and expectations. This quality plan defines the ASC program software quality practices and provides mappings of these practices to the SNL Corporate Process Requirements (CPR 1.3.2 and CPR 1.3.6) and the Department of Energy (DOE) document, ASCI Software Quality Engineering: Goals, Principles, and Guidelines (GP&G). This quality plan identifies ASC management andmore » software project teams' responsibilities for cost-effective software engineering quality practices. The SNL ASC Software Quality Plan establishes the signatories commitment to improving software products by applying cost-effective software engineering quality practices. This document explains the project teams opportunities for tailoring and implementing the practices; enumerates the practices that compose the development of SNL ASC's software products; and includes a sample assessment checklist that was developed based upon the practices in this document.« less
Advanced Stirling Convertor (ASC-E2) Characterization Testing

NASA Technical Reports Server (NTRS)

Williams, Zachary D.; Oriti, Salvatore M.

2012-01-01

Testing has been conducted on Advanced Stirling Convertors (ASCs)-E2 at NASA Glenn Research Center in support of the Advanced Stirling Radioisotope Generator (ASRG) project. This testing has been conducted to understand sensitivities of convertor parameters due to environmental and operational changes during operation of the ASRG in missions to space. This paper summarizes test results and explains the operation of the ASRG during space missions
Artificial Loading of ASC Specks with Cytosolic Antigens

PubMed Central

Sahillioğlu, Ali Can; Özören, Nesrin

2015-01-01

Inflammasome complexes form upon interaction of Nod Like Receptor (NLR) proteins with pathogen associated molecular patterns (PAPMS) inside the cytosol. Stimulation of a subset of inflammasome receptors including NLRP3, NLRC4 and AIM2 triggers formation of the micrometer-sized spherical supramolecular complex called the ASC speck. The ASC speck is thought to be the platform of inflammasome activity, but the reason why a supramolecular complex is preferred against oligomeric platforms remains elusive. We observed that a set of cytosolic proteins, including the model antigen ovalbumin, tend to co-aggregate on the ASC speck. We suggest that co-aggregation of antigenic proteins on the ASC speck during intracellular infection might be instrumental in antigen presentation. PMID:26258904
Advanced Stirling Convertor (ASC) - From Technology Development to Future Flight Product

NASA Technical Reports Server (NTRS)

Wong, Wayne A.; Wood, J. Gary; Wilson, Kyle

2008-01-01

The Advanced Stirling Convertor (ASC) is being developed by Sunpower, Inc. under contract to NASA s Glenn Research Center (GRC) with critical technology support tasks lead by GRC. The ASC development, funded by NASA s Science Mission Directorate, started in 2003 as one of 10 competitively awarded contracts that were to address future Radioisotope Power System (RPS) advanced power conversion needs. The ASC technology has since evolved through progressive convertor builds and successful testing to demonstrate high conversion efficiency (38 %), low mass (1.3 kg), hermetic sealing, launch vibration simulation, EMI characterization, and is undergoing extended operation. The GRC and Sunpower team recently delivered three ASC-E machines to the Department of Energy (DOE) and Lockheed Martin Space Systems Company, two units for integration onto the Advanced Stirling Radioisotope Generator Engineering Unit (ASRG EU) plus one spare. The design has recently been initiated for the ASC-E2, an evolution from the ASC-E that substitutes higher temperature materials enabling improved performance and higher reliability margins. This paper summarizes the history and status of the ASC project and discusses plans for this technology which enables RPS specific power of 8 W/kg for future NASA missions.
Advanced Stirling Convertor (ASC)--From Technology Development to Future Flight Product

NASA Technical Reports Server (NTRS)

Wong, Wayne A.; Wood, J. Gary; Wilson, Kyle

2008-01-01

The Advanced Stirling Convertor (ASC) is being developed by Sunpower Inc. under contract to NASA s Glenn Research Center (GRC) with critical technology support tasks led by GRC. The ASC development, funded by NASA s Science Mission Directorate, started in 2003 as one of 10 competitively awarded contracts that were intended to address the power conversion needs of future Radioisotope Power Systems (RPS). The ASC technology has since evolved through progressive convertor builds and successful testing to demonstrate high conversion efficiency (38 percent), low mass (1.3 kg), hermetic sealing, launch vibration simulation, EMI characterization, and is undergoing extended operation. The GRC and Sunpower team recently delivered two ASC-E convertors to the Department of Energy (DOE) and Lockheed Martin Space Systems Company for integration onto the Advanced Stirling Radioisotope Generator Engineering Unit (ASRG EU) plus one spare. The design of the next build, called the ASC-E2, has recently been initiated and is based on the heritage ASC-E with design refinements to increase reliability margin and offer higher temperature operation and improve performance. The ASC enables RPS system specific power of about 7 to 8 W/kg. This paper provides a chronology of ASC development to date and summarizes technical achievements including advancements toward flight implementation of the technology on ASRG by as early as 2013.
78 FR 15053 - Simpson Lumber Company, LLC, Shelton, Washington; Simpson Lumber Company, LLC, Tacoma, Washington...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-08

...,372B] Simpson Lumber Company, LLC, Shelton, Washington; Simpson Lumber Company, LLC, Tacoma, Washington; Simpson Lumber Company, LLC, Longview, Washington; Notice of Revised Determination on Reconsideration On... Reconsideration for the workers and former workers of Simpson Lumber Company, LLC, Shelton, Washington (TA-W-81...
ASC filament formation serves as a signal amplification mechanism for inflammasomes

PubMed Central

Dick, Mathias S.; Sborgi, Lorenzo; Rühl, Sebastian; Hiller, Sebastian; Broz, Petr

2016-01-01

A hallmark of inflammasome activation is the ASC speck, a micrometre-sized structure formed by the inflammasome adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD), which consists of a pyrin domain (PYD) and a caspase recruitment domain (CARD). Here we show that assembly of the ASC speck involves oligomerization of ASCPYD into filaments and cross-linking of these filaments by ASCCARD. ASC mutants with a non-functional CARD only assemble filaments but not specks, and moreover disrupt endogenous specks in primary macrophages. Systematic site-directed mutagenesis of ASCPYD is used to identify oligomerization-deficient ASC mutants and demonstrate that ASC speck formation is required for efficient processing of IL-1β, but dispensable for gasdermin-D cleavage and pyroptosis induction. Our results suggest that the oligomerization of ASC creates a multitude of potential caspase-1 activation sites, thus serving as a signal amplification mechanism for inflammasome-mediated cytokine production. PMID:27329339
Lateral Load Testing of the Advanced Stirling Convertor (ASC-E2) Heater Head

NASA Technical Reports Server (NTRS)

Cornell, Peggy A.; Krause, David L.; Davis, Glen; Robbie, Malcolm G.; Gubics, David A.

2010-01-01

Free-piston Stirling convertors are fundamental to the development of NASA s next generation of radioisotope power system, the Advanced Stirling Radioisotope Generator (ASRG). The ASRG will use General Purpose Heat Source (GPHS) modules as the energy source and Advanced Stirling Convertors (ASCs) to convert heat into electrical energy, and is being developed by Lockheed Martin under contract to the Department of Energy. Achieving flight status mandates that the ASCs satisfy design as well as flight requirements to ensure reliable operation during launch. To meet these launch requirements, GRC performed a series of quasi-static mechanical tests simulating the pressure, thermal, and external loading conditions that will be experienced by an ASC E2 heater head assembly. These mechanical tests were collectively referred to as lateral load tests since a primary external load lateral to the heater head longitudinal axis was applied in combination with the other loading conditions. The heater head was subjected to the operational pressure, axial mounting force, thermal conditions, and axial and lateral launch vehicle acceleration loadings. To permit reliable prediction of the heater head s structural performance, GRC completed Finite Element Analysis (FEA) computer modeling for the stress, strain, and deformation that will result during launch. The heater head lateral load test directly supported evaluation of the analysis and validation of the design to meet launch requirements. This paper provides an overview of each element within the test and presents assessment of the modeling as well as experimental results of this task.

Test Hardware Design for Flightlike Operation of Advanced Stirling Convertors (ASC-E3)

NASA Technical Reports Server (NTRS)

Oriti, Salvatore M.

2012-01-01

NASA Glenn Research Center (GRC) has been supporting development of the Advanced Stirling Radioisotope Generator (ASRG) since 2006. A key element of the ASRG project is providing life, reliability, and performance testing of the Advanced Stirling Convertor (ASC). For this purpose, the Thermal Energy Conversion branch at GRC has been conducting extended operation of a multitude of free-piston Stirling convertors. The goal of this effort is to generate long-term performance data (tens of thousands of hours) simultaneously on multiple units to build a life and reliability database. The test hardware for operation of these convertors was designed to permit in-air investigative testing, such as performance mapping over a range of environmental conditions. With this, there was no requirement to accurately emulate the flight hardware. For the upcoming ASC-E3 units, the decision has been made to assemble the convertors into a flight-like configuration. This means the convertors will be arranged in the dual-opposed configuration in a housing that represents the fit, form, and thermal function of the ASRG. The goal of this effort is to enable system level tests that could not be performed with the traditional test hardware at GRC. This offers the opportunity to perform these system-level tests much earlier in the ASRG flight development, as they would normally not be performed until fabrication of the qualification unit. This paper discusses the requirements, process, and results of this flight-like hardware design activity.
Detection of ASC Speck Formation by Flow Cytometry and Chemical Cross-linking.

PubMed

Hoss, Florian; Rolfes, Verena; Davanso, Mariana R; Braga, Tarcio T; Franklin, Bernardo S

2018-01-01

Assembly of a relatively large protein aggregate or "speck" formed by the adaptor protein ASC is a common downstream step in the activation of most inflammasomes. This unique feature of ASC allows its visualization by several imaging techniques and constitutes a reliable and feasible readout for inflammasome activation in cells and tissues. We have previously described step-by-step protocols to generate immortalized cell lines stably expressing ASC fused to a fluorescent protein for measuring inflammasome activation by confocal microscopy, and immunofluorescence of endogenous ASC in primary cells. Here, we present two more methods to detect ASC speck formation: (1) Assessment of ASC speck formation by flow cytometry; and (2) Chemical cross-linking of ASC followed by immunoblotting. These methods allow for the discrimination of inflammasome-activated versus non-activated cells, the identification of lineage-specific inflammasome activation in complex cell mixtures, and sorting of inflammasome-activated cells for further analysis.
[Adipose-derived stromal cells (ASC) - basics and therapeutic approaches in otorhinolaryngology].

PubMed

Frölich, K; Hagen, R; Kleinsasser, N

2014-06-01

Adipose-derived Stromal Cells (ASC) - Basics and Therapeutic Approaches in Otorhinolaryngology Mesenchymal stem cells from adipose tissue can be easily harvested with less discomfort, low donor-site morbidity and high amount compared to bone marrow-derived stem cells. Due to their multilineage differentiation potential in various cell types, immunmodulatory properties and their capability to enhance wound healing, ASC are a promising cell source for tissue engineering approaches and regenerative medicine. They are characterized by the expression of specific surface marker proteins and their differentiation potential into the mesenchymal lineages. Whereas only preclinical studies are published for otorhinolaryngology-related therapeutic options using ASC, various diseases, for instance graft-versus-host disease, have already been treated with ASC in single cases or clinical trials. Safety and genomic stability of ASC as well as the risk of spontaneous malignant transformation are still disputed. This review summarizes the current literature on characterization and anatomic localization of ASC. In addition, beside the presentation of preclinical studies concerning therapeutic approaches in otorhinolaryngology as well as of current clinical applications, the issue of safety of ASC in human stem cell therapy is discussed. © Georg Thieme Verlag KG Stuttgart · New York.
A Study of Civilian Registered Nurse Recruitment at Madigan Army Medical Center, Tacoma, Washington.

DTIC Science & Technology

1982-06-01

Marketing Management. Boston: Allyn and Bacon, Inc., 1980. Kotler , Philip . Marketing for Nonprofit Institutions. Englewood Cliffs, N.D.: Prentice-Hall...Interview with Ms. R. Marsh, Staffing Specialist, Force Develop- ment Division, Madigan Army Medical Center, Tacoma, WA (Dec 81) 20Philip Kotler ...Health Care Marketing: Issues and Trends, ed.: Philip D. Cooper (Germantown, MD.: Aspen Systems Corporation, 1979), p. 7. 22 Initial Report, National
One-year outcomes with the Taxus Liberté stent in the real world: the Taxus Olympia registry (phase I).

PubMed

Ahmed, Waqar Habib; Zambahari, Robaayah; Al-Rashdan, Ibrahim; Al Naeemi, Abdullah; Saeed, Fuad A; Mascioli, Stephen

2008-12-01

The Taxus Olympia registry is a prospective, postapproval registry collecting clinical outcomes data on patients receiving the Taxus Liberté paclitaxel-eluting stent during routine interventional cardiology practice. Between February and July 2005, 529 patients receiving the Taxus Liberté stent at 16 centers in the Middle East, South/Central America, and Asia/Pacific regions were enrolled in Phase I of Olympia. The primary end-point was Taxus Liberté stent-related cardiac events (cardiac death, MI, and revascularization) at 30 days postimplant. Additional clinical assessment was conducted at 6 and 12 months. Olympia phases II and III are in clinical follow-up and will be reported separately. One-year clinical follow-up is available for 98% of patients. Complex patients and lesions were prevalent, including: 50% diabetes mellitus, 49% multivessel disease, 30% multiple stenting, 48% AHA/ACC type B2/C lesions, 19% long lesions (>26 mm), and 40% small vessels (30 days postprocedure. One-year cardiac event rates among complex subpopulations (diabetics 5.0%, multiple stents 3.8%, long lesions 3.1%, and small vessels 2.9%) were comparable to the overall study population. In conclusion, this first report of real-world experience with the Taxus Liberté stent demonstrates the safety and clinical utility of this stent in the broader spectrum of coronary disease treated in everyday practice.
42 CFR 416.171 - Determination of payment rates for ASC services.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 3 2010-10-01 2010-10-01 false Determination of payment rates for ASC services... Determination of payment rates for ASC services. (a) Standard methodology. The standard methodology for determining the national unadjusted payment rate for ASC services is to calculate the product of the...
Clinical Relevance of ASC-H Cytologies: Experience in a Single Tertiary Hospital.

PubMed

Díaz Del Arco, Cristina; Sanabria Montoro, M Carmen; García López, Dolores; Rodríguez Escudero, Eva; Fernández Aceñero, M Jesús

2016-01-01

To review the cases of atypical squamous cells (ASC), cannot exclude squamous intraepithelial lesion (ASC-H) diagnosed at a single institution and to discuss the interpretation challenges and clinical management of these cases. The ASC-H cases diagnosed at our institution from 2006 to 2015 were studied retrospectively; of 159,000 Pap smears, there were 959 cases of ASC of undetermined significance (ASC-US) and 148 cases of ASC-H. We have reviewed the clinical records of the ASC-H cases, and data have been analyzed using SPSS 20.0. Median age was 43.1 years; 49.6% of the patients had previous cervical cytologies, and 55.9% of them were negative. Following ASC-H diagnosis, another smear was obtained in 97.1% of the cases and in 67.6% a biopsy was performed. Biopsy revealed low-grade dysplasia in 18.8% of the cases, high-grade dysplasia in 27.8%, and infiltrating carcinoma in 2.3%. Patients with significant lesions in histology were younger than patients with negative results (p = 0.08). A diagnosis of ASC-H/squamous intraepithelial lesion in the repeated cytology had a sensitivity of 74.2%, specificity of 39.6%, positive predictive value of 44.8%, and negative predictive value of 70% for the diagnosis of dysplasia. ASC-H diagnosis is associated with a high incidence of histological high-grade lesions. A repeat cytology can aid in the selection of high-risk patients, but there may be false-negative results. © 2016 S. Karger AG, Basel.
Can the Afinion HbA1c Point-of-Care instrument be an alternative method for the Tosoh G8 in the case of Hb-Tacoma?

PubMed

Lenters-Westra, Erna; Strunk, Annuska; Campbell, Paul; Slingerland, Robbert J

2017-02-01

Hb-variant interference when reporting HbA1c has been an ongoing challenge since HbA1c was introduced to monitor patients with diabetes mellitus. Most Hb-variants show an abnormal chromatogram when cation-exchange HPLC is used for the determination of HbA1c. Unfortunately, the Tosoh G8 generates what appears to be normal chromatogram in the presence of Hb-Tacoma, yielding a falsely high HbA1c value. The primary aim of the study was to investigate if the Afinion HbA1c point-of-care (POC) instrument could be used as an alternative method for the Tosoh G8 when testing for HbA1c in the presence of Hb-Tacoma. Whole blood samples were collected in K 2 EDTA tubes from individuals homozygous for HbA (n = 40) and heterozygous for Hb-Tacoma (n = 20). Samples were then immediately analyzed with the Afinion POC instrument. After analysis, aliquots of each sample were frozen at -80 °C. The frozen samples were shipped on dry ice to the European Reference Laboratory for Glycohemoglobin (ERL) and analyzed with three International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) and National Glycohemoglobin Standardization Program (NGSP) Secondary Reference Measurement Procedures (SRMPs). The Premier Hb9210 was used as the reference method. When compared to the reference method, samples with Hb-Tacoma yielded mean relative differences of 31.8% on the Tosoh G8, 21.5% on the Roche Tina-quant Gen. 2 and 16.8% on the Afinion. The Afinion cannot be used as an alternative method for the Tosoh G8 when testing for HbA1c in the presence of Hb-Tacoma.
77 FR 40636 - Northwest Hardwoods, Inc., Including On-Site Leased Workers of Manpower, Tacoma, WA; Notice of...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-10

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-81,314] Northwest Hardwoods, Inc., Including On-Site Leased Workers of Manpower, Tacoma, WA; Notice of Affirmative Determination Regarding... resulted in a negative determination based on the findings that there was no shift in production and there...
Biological Testing of Solid Phase and Suspended Phase Dredged Material from Commencement Bay, Tacoma, Washington

DTIC Science & Technology

1983-04-01

BAY, TACOMA, WASHINGTON PREPARED BY: FISHERIES RESEARCH INSTITUTE University of Washington DTIC C. A ELECTE JUL11 1985 DISTRIBUTIONSTATEMENT A...Nakatani 9. PERFORMING ORGANIZATION NAME AND ADDRESS 10. PROGRAM ELEMENT. PROJECT, TASK Fisheries Research Institute AREA & WORK UNIT NUMBERS School of... Fisheries WH-10 University of Washington Seattle, Washington 98195 11. CONTROLLING OFFICE NAME AND ADDRESS 12. REPORT DATE US Army Corps of Engineers
Materials Developed from American Indian Culture-Based Curriculum Workshop (Tacoma, Washington, October 24-28, 1978). Book Two.

ERIC Educational Resources Information Center

Disney, Dick, Comp.

Developed as a result of the second 5-day American Indian Culture-Based Curriculum Workshop conducted in Tacoma, Washington, the resource guide presents materials oriented toward Native American dance, music, and games, which were the major thrust of the workshop. The guide provides four flannelboard stories/legends (How Man Was Created, The Gull…
Develop Probabilistic Tsunami Design Maps for ASCE 7

NASA Astrophysics Data System (ADS)

Wei, Y.; Thio, H. K.; Chock, G.; Titov, V. V.

2014-12-01

A national standard for engineering design for tsunami effects has not existed before and this significant risk is mostly ignored in engineering design. The American Society of Civil Engineers (ASCE) 7 Tsunami Loads and Effects Subcommittee is completing a chapter for the 2016 edition of ASCE/SEI 7 Standard. Chapter 6, Tsunami Loads and Effects, would become the first national tsunami design provisions. These provisions will apply to essential facilities and critical infrastructure. This standard for tsunami loads and effects will apply to designs as part of the tsunami preparedness. The provisions will have significance as the post-tsunami recovery tool, to plan and evaluate for reconstruction. Maps of 2,500-year probabilistic tsunami inundation for Alaska, Washington, Oregon, California, and Hawaii need to be developed for use with the ASCE design provisions. These new tsunami design zone maps will define the coastal zones where structures of greater importance would be designed for tsunami resistance and community resilience. The NOAA Center for Tsunami Research (NCTR) has developed 75 tsunami inundation models as part of the operational tsunami model forecast capability for the U.S. coastline. NCTR, UW, and URS are collaborating with ASCE to develop the 2,500-year tsunami design maps for the Pacific states using these tsunami models. This ensures the probabilistic criteria are established in ASCE's tsunami design maps. URS established a Probabilistic Tsunami Hazard Assessment approach consisting of a large amount of tsunami scenarios that include both epistemic uncertainty and aleatory variability (Thio et al., 2010). Their study provides 2,500-year offshore tsunami heights at the 100-m water depth, along with the disaggregated earthquake sources. NOAA's tsunami models are used to identify a group of sources that produce these 2,500-year tsunami heights. The tsunami inundation limits and runup heights derived from these sources establish the tsunami design map
ASC-AD penetration modeling FY05 status report.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kistler, Bruce L.; Ostien, Jakob T.; Chiesa, Michael L.

2006-04-01

Sandia currently lacks a high fidelity method for predicting loads on and subsequent structural response of earth penetrating weapons. This project seeks to test, debug, improve and validate methodologies for modeling earth penetration. Results of this project will allow us to optimize and certify designs for the B61-11, Robust Nuclear Earth Penetrator (RNEP), PEN-X and future nuclear and conventional penetrator systems. Since this is an ASC Advanced Deployment project the primary goal of the work is to test, debug, verify and validate new Sierra (and Nevada) tools. Also, since this project is part of the V&V program within ASC, uncertaintymore » quantification (UQ), optimization using DAKOTA [1] and sensitivity analysis are an integral part of the work. This project evaluates, verifies and validates new constitutive models, penetration methodologies and Sierra/Nevada codes. In FY05 the project focused mostly on PRESTO [2] using the Spherical Cavity Expansion (SCE) [3,4] and PRESTO Lagrangian analysis with a preformed hole (Pen-X) methodologies. Modeling penetration tests using PRESTO with a pilot hole was also attempted to evaluate constitutive models. Future years work would include the Alegra/SHISM [5] and AlegrdEP (Earth Penetration) methodologies when they are ready for validation testing. Constitutive models such as Soil-and-Foam, the Sandia Geomodel [6], and the K&C Concrete model [7] were also tested and evaluated. This report is submitted to satisfy annual documentation requirements for the ASC Advanced Deployment program. This report summarizes FY05 work performed in the Penetration Mechanical Response (ASC-APPS) and Penetration Mechanics (ASC-V&V) projects. A single report is written to document the two projects because of the significant amount of technical overlap.« less
Quantification of Inflammasome Adaptor Protein ASC in Biological Samples by Multiple-Reaction Monitoring Mass Spectrometry.

PubMed

Ulke-Lemée, Annegret; Lau, Arthur; Nelson, Michelle C; James, Matthew T; Muruve, Daniel A; MacDonald, Justin A

2018-06-09

Inflammation is an integral component of many diseases, including chronic kidney disease (CKD). ASC (apoptosis-associated speck-like protein containing CARD, also PYCARD) is the key inflammasome adaptor protein in the innate immune response. Since ASC specks, a macromolecular condensate of ASC protein, can be released by inflammasome-activated cells into the extracellular space to amplify inflammatory responses, the ASC protein could be an important biomarker in diagnostic applications. Herein, we describe the development and validation of a multiple reaction monitoring mass spectrometry (MRM-MS) assay for the accurate quantification of ASC in human biospecimens. Limits of detection and quantification for the signature DLLLQALR peptide (used as surrogate for the target ASC protein) were determined by the method of standard addition using synthetic isotope-labeled internal standard (SIS) peptide and urine matrix from a healthy donor (LOQ was 8.25 pM, with a ~ 1000-fold linear range). We further quantified ASC in the urine of CKD patients (8.4 ± 1.3 ng ASC/ml urine, n = 13). ASC was positively correlated with proteinuria and urinary IL-18 in CKD samples but not with urinary creatinine. Unfortunately, the ASC protein is susceptible to degradation, and patient urine that was thawed and refrozen lost 85% of the ASC signal. In summary, the MRM-MS assay provides a robust means to quantify ASC in biological samples, including clinical biospecimens; however, sample collection and storage conditions will have a critical impact on assay reliability.
Absence of the inflammasome adaptor ASC reduces hypoxia-induced pulmonary hypertension in mice.

PubMed

Cero, Fadila Telarevic; Hillestad, Vigdis; Sjaastad, Ivar; Yndestad, Arne; Aukrust, Pål; Ranheim, Trine; Lunde, Ida Gjervold; Olsen, Maria Belland; Lien, Egil; Zhang, Lili; Haugstad, Solveig Bjærum; Løberg, Else Marit; Christensen, Geir; Larsen, Karl-Otto; Skjønsberg, Ole Henning

2015-08-15

Pulmonary hypertension is a serious condition that can lead to premature death. The mechanisms involved are incompletely understood although a role for the immune system has been suggested. Inflammasomes are part of the innate immune system and consist of the effector caspase-1 and a receptor, where nucleotide-binding oligomerization domain-like receptor pyrin domain-containing 3 (NLRP3) is the best characterized and interacts with the adaptor protein apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC). To investigate whether ASC and NLRP3 inflammasome components are involved in hypoxia-induced pulmonary hypertension, we utilized mice deficient in ASC and NLRP3. Active caspase-1, IL-18, and IL-1β, which are regulated by inflammasomes, were measured in lung homogenates in wild-type (WT), ASC(-/-), and NLRP3(-/-) mice, and phenotypical changes related to pulmonary hypertension and right ventricular remodeling were characterized after hypoxic exposure. Right ventricular systolic pressure (RVSP) of ASC(-/-) mice was significantly lower than in WT exposed to hypoxia (40.8 ± 1.5 mmHg vs. 55.8 ± 2.4 mmHg, P < 0.001), indicating a substantially reduced pulmonary hypertension in mice lacking ASC. Magnetic resonance imaging further supported these findings by demonstrating reduced right ventricular remodeling. RVSP of NLRP3(-/-) mice exposed to hypoxia was not significantly altered compared with WT hypoxia. Whereas hypoxia increased protein levels of caspase-1, IL-18, and IL-1β in WT and NLRP3(-/-) mice, this response was absent in ASC(-/-) mice. Moreover, ASC(-/-) mice displayed reduced muscularization and collagen deposition around arteries. In conclusion, hypoxia-induced elevated right ventricular pressure and remodeling were attenuated in mice lacking the inflammasome adaptor protein ASC, suggesting that inflammasomes play an important role in the pathogenesis of pulmonary hypertension. Copyright © 2015 the American Physiological
Study of Site Response in the Seattle and Tacoma Basins, Washington, Using Spectral Ratio Methods

NASA Astrophysics Data System (ADS)

Keshvardoost, R.; Wolf, L. W.

2014-12-01

Sedimentary basins are known to have a pronounced influence on earthquake-generated ground motions, affecting both predominant frequencies and wave amplification. These site characteristics are important elements in estimating ground shaking and seismic hazard. In this study, we use three-component broadband and strong motion seismic data from three recent earthquakes to determine site response characteristics in the Seattle and Tacoma basins, Washington. Resonant frequencies and relative amplification of ground motions were determined using Fourier spectral ratios of velocity and acceleration records from the 2012 Mw 6.1 Vancouver Island earthquake, the 2012 Mw 7.8 Queen Charlotte Island earthquake, and the 2014 Mw 6.6 Vancouver Island earthquake. Recordings from sites within and adjacent to the Seattle and Tacoma basins were selected for the study based on their signal to noise ratios. Both the Standard Spectral Ratio (SSR) and the Horizontal-to-Vertical Spectral Ratio (HVSR) methods were used in the analysis, and results from each were compared to examine their agreement and their relation to local geology. Although 57% of the sites (27 out of 48) exhibited consistent results between the two methods, other sites varied considerably. In addition, we use data from the Seattle Liquefaction Array (SLA) to evaluate the site response at 4 different depths. Results indicate that resonant frequencies remain the same at different depths but amplification decreases significantly over the top 50 m.
A Preliminary evaluation of hydrology and water quality near the Tacoma Landfill, Pierce County, Washington

USGS Publications Warehouse

Lum, W.E.; Turney, G.L.

1985-01-01

The Tacoma landfill, located in western Pierce County, Washington, has been used for the disposal of waste since about 1960. Disposal operations are planned to continue at this site until at least 1990. Data were compiled and interpreted to help understand the possible effects of the landfill on water quality in the surrounding area. Data were collected from published and unpublished reports of the U.S. Geological Survey, and from predominantly unpublished data in the files of other government agencies. The Tacoma landfill is underlain by unconsolidated, glacially derived deposits that consist of a wide variety of mixtures of clay to boulder-sized materials. Ground water is mostly the result of rainfall on the land surface, and moves through artesian aquifers (under the landfill) that are tapped for both domestic and municipal use. Hazardous liquid and dissolved wastes are probably present in the landfill, and potential flow paths for waste migration exist. An undetermined number of single-family domestic wells and 18 public-supply wells are within 3 miles of the landfill, three as close as 0.2 miles. There is only limited evidence indicating ground- and surface-water contamination. Further investigations of the geology, hydrology and water quality are needed to characterize the impact the landfill has on ground- and surface-water of the surrounding area. (USGS)
Autonomous osteogenic differentiation of hASCs encapsulated in methacrylated gellan-gum hydrogels.

PubMed

Oliveira, Mariana B; Custódio, Catarina A; Gasperini, Luca; Reis, Rui L; Mano, João F

2016-09-01

Methacrylated gellan-gum (GG-MA) alone and combined with collagen type I (Coll) is suggested here for the first time as a cell-laden injectable biomaterial for bone regeneration. On-chip high-throughput studies allowed rapidly assessing the suitability of 15 biomaterials/media combinations for the osteodifferentiation of human adipose stem cells (hASCs). Hydrogels composed solely of GG-MA (GG100:0Coll) led hASCs from three different donors into the osteogenic lineage after 21days of cell culture, in the absence of any osteogenic or osteoconductive factors. Hydrogels containing more than 30% of Coll promoted increased cellular proliferation and led hASCs into osteogenic differentiation under basal conditions. Studies using isolated individual hydrogels - excluding eventual on-chip crosstalk - and standard biochemical assays corroborated such findings. The formation of focal adhesions of hASCs on GG100:0Coll hydrogels was verified. We hypothesize that the hydrogels osteogenic effect could be guided by mechanotransduction phenomena. Indeed, the hydrogels showed elastic modulus in ranges previously reported as osteoinductive and the inhibition of the actin-myosin contractility pathway impaired hASCs' osteodifferentiation. GG-MA hydrogels also did not promote hASCs' adipogenesis while used in basal conditions. Overall, GG-MA showed promising properties as an innovative and off-the shelf self-inducing osteogenic injectable biomaterial. Methacrylated gellan gum (GG-MA) is here suggested for the first time as a widely available polysaccharide to easily prepare hydrogels with cell adhesion properties and capability of inducing the autonomous osteogenic differentiation of human adipose-derived stem cells (hASCs). GG-MA was processed as stand-alone hydrogels or in different combinations with collage type I. All hydrogel formulations elicited the osteogenic differentiation of hASCs, independently of the addition of any osteoconductive or osteogenic stimuli, i.e. in basal
The emerging role of ASC in dendritic cell metabolism during Chlamydia infection

PubMed Central

McKeithen, Danielle N.; Ryans, Khamia; Mu, Jing; Xie, Zhonglin; Simoneaux, Tankya; Blas-machado, Uriel; Eko, Francis O.; Black, Carolyn M.; Igietseme, Joseph U.; He, Qing

2017-01-01

Chlamydia trachomatis is a bacterial agent that causes sexually transmitted infections worldwide. The regulatory functions of dendritic cells (DCs) play a major role in protective immunity against Chlamydia infections. Here, we investigated the role of ASC in DCs metabolism and the regulation of DCs activation and function during Chlamydia infection. Following Chlamydia stimulation, maturation and antigen presenting functions were impaired in ASC-/- DCs compared to wild type (WT) DCs, in addition, ASC deficiency induced a tolerant phenotype in Chlamydia stimulated DCs. Using real-time extracellular flux analysis, we showed that activation in Chlamydia stimulated WT DCs is associated with a metabolic change in which mitochondrial oxidative phosphorylation (OXPHOS) is inhibited and the cells become committed to utilizing glucose through aerobic glycolysis for differentiation and antigen presenting functions. However, in ASC-/- DCs Chlamydia-induced metabolic change was prevented and there was a significant effect on mitochondrial morphology. The mitochondria of Chlamydia stimulated ASC-/- DCs had disrupted cristae compared to the normal narrow pleomorphic cristae found in stimulated WT DCs. In conclusion, our results suggest that Chlamydia-mediated activation of DCs is associated with a metabolic transition in which OXPHOS is inhibited, thereby dedicating the DCs to aerobic glycolysis, while ASC deficiency disrupts DCs function by inhibiting the reprogramming of DCs metabolism within the mitochondria, from glycolysis to electron transport chain. PMID:29216217
ASC Induces Apoptosis via Activation of Caspase-9 by Enhancing Gap Junction-Mediated Intercellular Communication

PubMed Central

Hida, Shigeaki; Fujii, Chifumi; Taniguchi, Shun’ichiro; Ito, Kensuke; Matsumura, Tomio; Okada, Nagisa; Sakaizawa, Takashi; Kobayashi, Akira; Takeoka, Michiko; Miyagawa, Shin-ichi

2017-01-01

ASC (apoptosis-associated speck-like protein containing a CARD) is a key adaptor molecule of inflammasomes that mediates inflammatory and apoptotic signals. Aberrant methylation-induced silencing of ASC has been observed in a variety of cancer cells, thus implicating ASC in tumor suppression, although this role remains incompletely defined especially in the context of closely neighboring cell proliferation. As ASC has been confirmed to be silenced by abnormal methylation in HT1080 fibrosarcoma cells as well, this cell line was investigated to characterize the precise role and mechanism of ASC in tumor progression. The effects of ASC were examined using in vitro cell cultures based on comparisons between low and high cell density conditions as well as in a xenograft murine model. ASC overexpression was established by insertion of the ASC gene into pcDNA3 and pMX-IRES-GFP vectors, the latter being packed into a retrovirus and subjected to reproducible competitive assays using parental cells as an internal control, for evaluation of cell viability. p21 and p53 were silenced using shRNA. Cell viability was suppressed in ASC-expressing transfectants as compared with control cells at high cell density conditions in in vitro culture and colony formation assays and in in vivo ectopic tumor formation trials. This suppression was not detected in low cell density conditions. Furthermore, remarkable progression of apoptosis was observed in ASC-introduced cells at a high cell density, but not at a low one. ASC-dependent apoptosis was mediated not by p21, p53, or caspase-1, but rather by cleavage of caspase-9 as well as by suppression of the NF-κB-related X-linked inhibitor-of-apoptosis protein. Caspase-9 cleavage was observed to be dependent on gap junction formation. The remarkable effect of ASC on the induction of apoptosis through caspase-9 and gap junctions revealed in this study may lead to promising new approaches in anticancer therapy. PMID:28056049

The Tacoma Narrows Bridge Collapse on Film and Video

NASA Astrophysics Data System (ADS)

Olson, Don; Hook, Joseph; Doescher, Russell; Wolf, Steven

2015-11-01

This month marks the 75th anniversary of the Tacoma Narrows Bridge collapse. During a gale on Nov. 7, 1940, the bridge exhibited remarkable oscillations before collapsing spectacularly (Figs. 1-5). Physicists over the years have spent a great deal of time and energy studying this event. By using open-source analysis tools and digitized footage of the disaster, physics students in both high school and college can continue in this tradition. Students can watch footage of "Galloping Gertie," ask scientific questions about the bridge's collapse, analyze data, and draw conclusions from that analysis. Students should be encouraged to pursue their own investigations, but the question that drove our inquiry was this: "When physics classes watch modern video showing the oscillations and the free fall of the bridge fragments, are these scenes sped up, slowed down, or at the correct speed compared to what was observed by the eyewitnesses on Nov. 7, 1940?"
Single-Molecule Fluorescence Reveals the Oligomerization and Folding Steps Driving the Prion-like Behavior of ASC.

PubMed

Gambin, Yann; Giles, Nichole; O'Carroll, Ailís; Polinkovsky, Mark; Hunter, Dominic; Sierecki, Emma

2018-02-16

Single-molecule fluorescence has the unique ability to quantify small oligomers and track conformational changes at a single-protein level. Here we tackled one of the most extreme protein behaviors, found recently in an inflammation pathway. Upon danger recognition in the cytosol, NLRP3 recruits its signaling adaptor, ASC. ASC start polymerizing in a prion-like manner and the system goes in "overdrive" by producing a single micron-sized "speck." By precisely controlling protein expression levels in an in vitro translation system, we could trigger the polymerization of ASC and mimic formation of specks in the absence of inflammasome nucleators. We utilized single-molecule spectroscopy to fully characterize prion-like behaviors and self-propagation of ASC fibrils. We next used our controlled system to monitor the conformational changes of ASC upon fibrillation. Indeed, ASC consists of a PYD and CARD domains, separated by a flexible linker. Individually, both domains have been found to form fibrils, but the structure of the polymers formed by the full-length ASC proteins remains elusive. For the first time, using single-molecule Förster resonance energy transfer, we studied the relative positions of the CARD and PYD domains of full-length ASC. An unexpectedly large conformational change occurred upon ASC fibrillation, suggesting that the CARD domain folds back onto the PYD domain. However, contradicting current models, the "prion-like" conformer was not initiated by binding of ASC to the NLRP3 platform. Rather, using a new method, hybrid between Photon Counting Histogram and Number and Brightness analysis, we showed that NLRP3 forms hexamers with self-binding affinities around 300nM. Overall our data suggest a new mechanism, where NLRP3 can initiate ASC polymerization simply by increasing the local concentration of ASC above a supercritical level. Copyright © 2017. Published by Elsevier Ltd.
Aeroacoustics research in Europe: The CEAS-ASC report on 2016 highlights

NASA Astrophysics Data System (ADS)

Wilson, Alexander G.

2018-08-01

The Council of European Aerospace Societies (CEAS) Aeroacoustics Specialists Committee (ASC) supports and promotes the interests of the scientific and industrial aeroacoustics community on the European scale, and European aeronautics activities internationally. Each year, the committee highlights several of the research and development projects in Europe. This paper is the 2016 issue of this collection of Aeroacoustic Highlights, compiled from contributions submitted to the CEAS-ASC. The contributions are classified under three main headings; Aircraft and Turbomachinery Noise, Experimental and Numerical Methods and Further Applications of Aeroacoustics. A concise summary of the CEAS-ASC workshop held in Southampton, England, in September 2016 is also included in this report.
Pre-Milestone I Program Development Process Guide (ASC/YX).

DTIC Science & Technology

1993-11-01

the PMD tasking to a particular Center. In the case of ASC, the task is entered into the ASC New Work Review process for internal allocation. AFMC/XP...0 Prooect Team to support decisions they make in response to external and - - internal requirements. Data base inputs 1 I"" 11. 12 0EVEFJ.O RA" DW...other international capabilities (COD). Although these are considered during the pre-MS 0 tasks (Task 0.2), they are examined once again during the
Ultrasound -Assisted Gene Transfer to Adipose Tissue-Derived Stem/Progenitor Cells (ASCs)

NASA Astrophysics Data System (ADS)

Miyamoto, Yoshitaka; Ueno, Hitomi; Hokari, Rei; Yuan, Wenji; Kuno, Shuichi; Kakimoto, Takashi; Enosawa, Shin; Negishi, Yoichi; Yoshinaka, Kiyoshi; Matsumoto, Yoichiro; Chiba, Toshio; Hayashi, Shuji

2011-09-01

In recent years, multilineage adipose tissue-derived stem cells (ASCs) have become increasingly attractive as a promising source for cell transplantation and regenerative medicine. Particular interest has been expressed in the potential to make tissue stem cells, such as ASCs and marrow stromal cells (MSCs), differentiate by gene transfection. Gene transfection using highly efficient viral vectors such as adeno- and sendai viruses have been developed for this purpose. Sonoporation, or ultrasound (US)-assisted gene transfer, is an alternative gene manipulation technique which employs the creation of a jet stream by ultrasonic microbubble cavitation. Sonoporation using non-viral vectors is expected to be a much safer, although less efficient, tool for prospective clinical gene therapy. In this report, we assessed the efficacy of the sonoporation technique for gene transfer to ASCs. We isolated and cultured adipocyets from mouse adipose tissue. ASCs that have the potential to differentiate with transformation into adipocytes or osteoblasts were obtained. Using the US-assisted system, plasmid DNA containing beta-galactosidase (beta-Gal) and green fluorescent protein (GFP) genes were transferred to the ASCs. For this purpose, a Sonopore 4000 (NEPAGENE Co.) and a Sonazoid (Daiichi Sankyo Co.) instrument were used in combination. ASCs were subjected to US (3.1 MHz, 50% duty cycle, burst rate 2.0 Hz, intensity 1.2 W/cm2, exposure time 30 sec). We observed that the gene was more efficiently transferred with increased concentrations of plasmid DNA (5-150 μg/mL). However, further optimization of the US parameters is required, as the gene transfer efficiency was still relatively low. In conclusion, we herein demonstrate that a gene can be transferred to ASCs using our US-assisted system. In regenerative medicine, this system might resolve the current issues surrounding the use of viral vectors for gene transfer.
ASC Methylation and Interleukin-1β Are Associated with Aerobic Capacity in Heart Failure.

PubMed

Butts, Brittany; Butler, Javed; Dunbar, Sandra B; Corwin, Elizabeth J; Gary, Rebecca A

2017-06-01

Aerobic capacity, as measured by peak oxygen uptake (V˙O2), is one of the most powerful predictors of prognosis in heart failure (HF). Inflammation is a key factor contributing to alterations in aerobic capacity, and interleukin (IL)-1 cytokines are implicated in this process. The adaptor protein ASC is necessary for inflammasome activation of IL-1β and IL-18. ASC expression is controlled through epigenetic modification; lower ASC methylation is associated with worse outcomes in HF. The purpose of this study is to examine the relationships between ASC methylation, IL-1β, and IL-18 with V˙O2peak in persons with HF. This study examined the relationship between ASC methylation, IL-1β, and IL-18 with V˙O2peak in 54 stable outpatients with HF. All participants were NYHA class II or III, not engaged in an exercise program, and physically able to complete an exercise treadmill test. Mean V˙O2peak was 16.68 ± 4.7 mL·kg·min. V˙O2peak was positively associated with mean percent ASC methylation (r = 0.47, P = 0.001) and negatively associated with IL-1β (r = -0.38, P = 0.007). Multiple linear regression models demonstrated that V˙O2peak increased by 2.30 mL·kg·min for every 1% increase in ASC methylation and decreased by 1.91 mL·kg·min for every 1 pg·mL increase in plasma IL-1β. Mean percent ASC methylation and plasma IL-1β levels are associated with clinically meaningful differences in V˙O2peak in persons with HF. Inflammasome activation may play a mechanistic role in determining aerobic capacity. ASC methylation is a potentially modifiable mechanism for reducing the inflammatory response, thereby improving aerobic capacity in HF.
Evaluating Energy Savings in All-Electric Public Housing in the Pacific Northwest, Tacoma, Washington (Fact Sheet)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

2014-03-01

This project analyzes the cost effectiveness of energy savings measures installed by a large public housing authority in Salishan, a community in Tacoma Washington. Research focuses on the modeled and measured energy usage of the first six phases of construction, and compares the energy usage of those phases to phase 7. Market-ready energy solutions were also evaluated to improve the efficiency of affordable housing for new and existing (built since 2001) affordable housing in the marine climate of Washington State.
Prevalence of cervical intraepithelial neoplasia grades II/III and cervical cancer in patients with cytological diagnosis of atypical squamous cells when high-grade intraepithelial lesions (ASC-H) cannot be ruled out.

PubMed

Cytryn, Andréa; Russomano, Fábio Bastos; Camargo, Maria José de; Zardo, Lucília Maria Gama; Horta, Nilza Maria Sobral Rebelo; Fonseca, Rachel de Carvalho Silveira de Paula; Tristão, Maria Aparecida; Monteiro, Aparecida Cristina Sampaio

2009-09-01

The latest update of the Bethesda System divided the category of atypical squamous cells of undetermined significance (ASCUS) into ASC-US (undetermined significance) and ASC-H (high-grade intraepithelial lesion cannot be ruled out). The aims here were to measure the prevalence of pre-invasive lesions (cervical intraepithelial neoplasia, CIN II/III) and cervical cancer among patients referred to Instituto Fernandes Figueira (IFF) with ASC-H cytology, and compare them with ASC-US cases. Cross-sectional study with retrospective data collection, at the IFF Cervical Pathology outpatient clinic. ASCUS cases referred to IFF from November 1997 to September 2007 were reviewed according to the 2001 Bethesda System to reach cytological consensus. The resulting ASC-H and ASC-US cases, along with new cases, were analyzed relative to the outcome of interest. The histological diagnosis (or cytocolposcopic follow-up in cases without such diagnosis) was taken as the gold standard. The prevalence of CIN II/III in cases with ASC-H cytology was 19.29% (95% confidence interval, CI, 9.05-29.55%) and the risk of these lesions was greater among patients with ASC-H than with ASC-US cytology (prevalence ratio, PR, 10.42; 95% CI, 2.39-45.47; P = 0.0000764). Pre-invasive lesions were more frequently found in patients under 50 years of age with ASC-H cytology (PR, 2.67; 95% CI, 0.38-18.83); P = 0.2786998). There were no uterine cervical cancer cases. The prevalence of CIN II/III in patients with ASC-H cytology was significantly higher than with ASC-US, and division into ASC diagnostic subcategories had good capacity for discriminating the presence of pre-invasive lesions.
Dune field pattern formation and recent transporting winds in the Olympia Undae Dune Field, north polar region of Mars

NASA Astrophysics Data System (ADS)

Ewing, Ryan C.; Peyret, Aymeric-Pierre B.; Kocurek, Gary; Bourke, Mary

2010-08-01

High-Resolution Imaging Science Experiment (HiRISE) imagery of the central Olympia Undae Dune Field in the north polar region of Mars shows a reticulate dune pattern consisting of two sets of nearly orthogonal dune crestlines, with apparent slipfaces on the primary crests, ubiquitous wind ripples, areas of coarse-grained wind ripples, and deflated interdune areas. Geomorphic evidence and dune field pattern analysis of dune crest length, spacing, defect density, and orientation indicates that the pattern is complex, representing two constructional generations of dunes. The oldest and best-organized generation forms the primary crestlines and is transverse to circumpolar easterly winds. Gross bed form-normal analysis of the younger pattern of crestlines indicates that it emerged with both circumpolar easterly winds and NE winds and is reworking the older pattern. Mapping of secondary flow fields over the dunes indicates that the most recent transporting winds were from the NE. The younger pattern appears to represent an influx of sediment to the dune field associated with the development of the Olympia Cavi reentrant, with NE katabatic winds channeling through the reentrant. A model of the pattern reformation based upon the reconstructed primary winds and resulting secondary flow fields shows that the development of the secondary pattern is controlled by the boundary condition of the older dune topography.
ASC-ATDM Performance Portability Requirements for 2015-2019

DOE Office of Scientific and Technical Information (OSTI.GOV)

Edwards, Harold C.; Trott, Christian Robert

This report outlines the research, development, and support requirements for the Advanced Simulation and Computing (ASC ) Advanced Technology, Development, and Mitigation (ATDM) Performance Portability (a.k.a., Kokkos) project for 2015 - 2019 . The research and development (R&D) goal for Kokkos (v2) has been to create and demonstrate a thread - parallel programming model a nd standard C++ library - based implementation that enables performance portability across diverse manycore architectures such as multicore CPU, Intel Xeon Phi, and NVIDIA Kepler GPU. This R&D goal has been achieved for algorithms that use data parallel pat terns including parallel - for, parallelmore » - reduce, and parallel - scan. Current R&D is focusing on hierarchical parallel patterns such as a directed acyclic graph (DAG) of asynchronous tasks where each task contain s nested data parallel algorithms. This five y ear plan includes R&D required to f ully and performance portably exploit thread parallelism across current and anticipated next generation platforms (NGP). The Kokkos library is being evaluated by many projects exploring algorithm s and code design for NGP. Some production libraries and applications such as Trilinos and LAMMPS have already committed to Kokkos as their foundation for manycore parallelism an d performance portability. These five year requirements includes support required for current and antic ipated ASC projects to be effective and productive in their use of Kokkos on NGP. The greatest risk to the success of Kokkos and ASC projects relying upon Kokkos is a lack of staffing resources to support Kokkos to the degree needed by these ASC projects. This support includes up - to - date tutorials, documentation, multi - platform (hardware and software stack) testing, minor feature enhancements, thread - scalable algorithm consulting, and managing collaborative R&D.« less
Building America Case Study: Performance and Costs of Ductless Heat Pumps in Marine Climate High-Performance Homes: Habitat for Humanity -- The Woods, Tacoma, Washington

DOE Office of Scientific and Technical Information (OSTI.GOV)

The Woods is a Habitat for Humanity (HFH) community of ENERGY STAR Homes (c) Northwest (ESHNW)-certified homes located in the marine climate of Tacoma/Pierce County, Washington. This research report builds on an earlier preliminary draft 2014 BA report, and includes significant billing analysis and cost effectiveness research from a collaborative, ongoing Ductless Heat Pump (DHP) research effort for Tacoma Public Utilities (TPU) and Bonneville Power Administration (BPA). This report focuses on the results of field testing, modeling, and monitoring of ductless mini-split heat pump hybrid heating systems in seven homes built and first occupied at various times between September 2013more » and October 2014. The report also provides WSU documentation of high-performance home observations, lessons learned, and stakeholder recommendations for builders of affordable high-performance housing such as HFH.« less
Required number of records for ASCE/SEI 7 ground-motion scaling procedure

USGS Publications Warehouse

Reyes, Juan C.; Kalkan, Erol

2011-01-01

The procedures and criteria in 2006 IBC (International Council of Building Officials, 2006) and 2007 CBC (International Council of Building Officials, 2007) for the selection and scaling ground-motions for use in nonlinear response history analysis (RHA) of structures are based on ASCE/SEI 7 provisions (ASCE, 2005, 2010). According to ASCE/SEI 7, earthquake records should be selected from events of magnitudes, fault distance, and source mechanisms that comply with the maximum considered earthquake, and then scaled so that the average value of the 5-percent-damped response spectra for the set of scaled records is not less than the design response spectrum over the period range from 0.2Tn to 1.5Tn sec (where Tn is the fundamental vibration period of the structure). If at least seven ground-motions are analyzed, the design values of engineering demand parameters (EDPs) are taken as the average of the EDPs determined from the analyses. If fewer than seven ground-motions are analyzed, the design values of EDPs are taken as the maximum values of the EDPs. ASCE/SEI 7 requires a minimum of three ground-motions. These limits on the number of records in the ASCE/SEI 7 procedure are based on engineering experience, rather than on a comprehensive evaluation. This study statistically examines the required number of records for the ASCE/SEI 7 procedure, such that the scaled records provide accurate, efficient, and consistent estimates of" true" structural responses. Based on elastic-perfectly-plastic and bilinear single-degree-of-freedom systems, the ASCE/SEI 7 scaling procedure is applied to 480 sets of ground-motions. The number of records in these sets varies from three to ten. The records in each set were selected either (i) randomly, (ii) considering their spectral shapes, or (iii) considering their spectral shapes and design spectral-acceleration value, A(Tn). As compared to benchmark (that is, "true") responses from unscaled records using a larger catalog of ground
Structure and assembly of the mouse ASC inflammasome by combined NMR spectroscopy and cryo-electron microscopy

PubMed Central

Sborgi, Lorenzo; Ravotti, Francesco; Dandey, Venkata P.; Dick, Mathias S.; Mazur, Adam; Reckel, Sina; Chami, Mohamed; Scherer, Sebastian; Huber, Matthias; Böckmann, Anja; Egelman, Edward H.; Stahlberg, Henning; Broz, Petr; Meier, Beat H.; Hiller, Sebastian

2015-01-01

Inflammasomes are multiprotein complexes that control the innate immune response by activating caspase-1, thus promoting the secretion of cytokines in response to invading pathogens and endogenous triggers. Assembly of inflammasomes is induced by activation of a receptor protein. Many inflammasome receptors require the adapter protein ASC [apoptosis-associated speck-like protein containing a caspase-recruitment domain (CARD)], which consists of two domains, the N-terminal pyrin domain (PYD) and the C-terminal CARD. Upon activation, ASC forms large oligomeric filaments, which facilitate procaspase-1 recruitment. Here, we characterize the structure and filament formation of mouse ASC in vitro at atomic resolution. Information from cryo-electron microscopy and solid-state NMR spectroscopy is combined in a single structure calculation to obtain the atomic-resolution structure of the ASC filament. Perturbations of NMR resonances upon filament formation monitor the specific binding interfaces of ASC-PYD association. Importantly, NMR experiments show the rigidity of the PYD forming the core of the filament as well as the high mobility of the CARD relative to this core. The findings are validated by structure-based mutagenesis experiments in cultured macrophages. The 3D structure of the mouse ASC-PYD filament is highly similar to the recently determined human ASC-PYD filament, suggesting evolutionary conservation of ASC-dependent inflammasome mechanisms. PMID:26464513
Comparison of Simulated Microgravity and Hydrostatic Pressure for Chondrogenesis of hASC.

PubMed

Mellor, Liliana F; Steward, Andrew J; Nordberg, Rachel C; Taylor, Michael A; Loboa, Elizabeth G

2017-04-01

Cartilage tissue engineering is a growing field due to the lack of regenerative capacity of native tissue. The use of bioreactors for cartilage tissue engineering is common, but the results are controversial. Some studies suggest that microgravity bioreactors are ideal for chondrogenesis, while others show that mimicking hydrostatic pressure is crucial for cartilage formation. A parallel study comparing the effects of loading and unloading on chondrogenesis has not been performed. The goal of this study was to evaluate chondrogenesis of human adipose-derived stem cells (hASC) under two different mechanical stimuli relative to static culture: microgravity and cyclic hydrostatic pressure (CHP). Pellets of hASC were cultured for 14 d under simulated microgravity using a rotating wall vessel bioreactor or under CHP (7.5 MPa, 1 Hz, 4 h · d-1) using a hydrostatic pressure vessel. We found that CHP increased mRNA expression of Aggrecan, Sox9, and Collagen II, caused a threefold increase in sulfated glycosaminoglycan production, and resulted in stronger vimentin staining intensity and organization relative to microgravity. In addition, Wnt-signaling patterns were altered in a manner that suggests that simulated microgravity decreases chondrogenic differentiation when compared to CHP. Our goal was to compare chondrogenic differentiation of hASC using a microgravity bioreactor and a hydrostatic pressure vessel, two commonly used bioreactors in cartilage tissue engineering. Our results indicate that CHP promotes hASC chondrogenesis and that microgravity may inhibit hASC chondrogenesis. Our findings further suggest that cartilage formation and regeneration might be compromised in space due to the lack of mechanical loading.Mellor LF, Steward AJ, Nordberg RC, Taylor MA, Loboa EG. Comparison of simulated microgravity and hydrostatic pressure for chondrogenesis of hASC. Aerosp Med Hum Perform. 2017; 88(4):377-384.
76 FR 27668 - ASC Machine Tools, Inc., Spokane Valley, WA; Notice of Negative Determination on Reconsideration

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-12

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-72,971] ASC Machine Tools, Inc... workers and former workers of ASC Machine Tools, Inc., Spokane Valley, Washington (the subject firm). The... workers of ASC Machine Tools, Inc., Spokane Valley, Washington. Signed in Washington, DC, on this 2nd day...
Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model.

PubMed

Kato, Yuka; Iwata, Takanori; Washio, Kaoru; Yoshida, Toshiyuki; Kuroda, Hozue; Morikawa, Shunichi; Hamada, Mariko; Ikura, Kazuki; Kaibuchi, Nobuyuki; Yamato, Masayuki; Okano, Teruo; Uchigata, Yasuko

2017-08-04

Artificial skin has achieved considerable therapeutic results in clinical practice. However, artificial skin treatments for wounds in diabetic patients with impeded blood flow or with large wounds might be prolonged. Cell-based therapies have appeared as a new technique for the treatment of diabetic ulcers, and cell-sheet engineering has improved the efficacy of cell transplantation. A number of reports have suggested that adipose-derived stem cells (ASCs), a type of mesenchymal stromal cell (MSC), exhibit therapeutic potential due to their relative abundance in adipose tissue and their accessibility for collection when compared to MSCs from other tissues. Therefore, ASCs appear to be a good source of stem cells for therapeutic use. In this study, ASC sheets from the epididymal adipose fat of normal Lewis rats were successfully created using temperature-responsive culture dishes and normal culture medium containing ascorbic acid. The ASC sheets were transplanted into Zucker diabetic fatty (ZDF) rats, a rat model of type 2 diabetes and obesity, that exhibit diminished wound healing. A wound was created on the posterior cranial surface, ASC sheets were transplanted into the wound, and a bilayer artificial skin was used to cover the sheets. ZDF rats that received ASC sheets had better wound healing than ZDF rats without the transplantation of ASC sheets. This approach was limited because ASC sheets are sensitive to dry conditions, requiring the maintenance of a moist wound environment. Therefore, artificial skin was used to cover the ASC sheet to prevent drying. The allogenic transplantation of ASC sheets in combination with artificial skin might also be applicable to other intractable ulcers or burns, such as those observed with peripheral arterial disease and collagen disease, and might be administered to patients who are undernourished or are using steroids. Thus, this treatment might be the first step towards improving the therapeutic options for diabetic wound
Experimental study of ASCs combined with POC-PLA patch for the reconstruction of full-thickness chest wall defects.

PubMed

Zhang, Yuanzheng; Fang, Shuo; Dai, Jiezhi; Zhu, Lei; Fan, Hao; Tang, Weiya; Fan, Yongjie; Dai, Haiying; Zhang, Peipei; Wang, Ying; Xing, Xin; Yang, Chao

2017-01-01

To explore the repairing effect of combination of adipose stem cells (ASCs) and composite scaffolds on CWR, the electrospun Poly 1, 8-octanediol-co-citric acid (POC)-poly-L-lactide acid (PLA) composite scaffolds were prepared, followed by in vitro and in vivo biocompatibility evaluation of the scaffolds. Afterwards, ASCs were seeded on POC-PLA to construct the POC-PLA-ASCs scaffolds, and the POC-PLA, POC-PLA-ASCs, and traditional materials expanded polytetrafluoroethylene (ePTFE) were adopt for CWR in New Zealand white (NZW) rabbit models. As results, the POC-PLA-ASCs patches possessed good biocompatibility as the high proliferation ability of cells surrounding the patches. Rabbits in POC-PLA-ASCs groups showed better pulmonary function, less pleural adhesion, higher degradation rate and more neovascularization when compared with that in other two groups. The results of western blot indicated that POC-PLA-ASCs patches accelerated the expression of VEGF and Collagen I in rabbit models. From the above, our present study demonstrated that POC-PLA material was applied for CWR successfully, and ASCs seeded on the sheets could improve the pleural adhesions and promote the reparation of chest wall defects.
Habitat Evaluation Procedures (HEP) Report; Tacoma/Trimble Area Management Plan, Technical Report 2001-2003.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Entz, Ray; Lockwood, Jr., Neil; Holmes, Darren

2003-10-01

In 2000 and 2001, the Kalispel Natural Resource Department (KNRD) continued to mitigate the wildlife habitat losses as part of the Albeni Falls Wildlife Mitigation Project. Utilizing Bonneville Power Administration (BPA) funds, the Kalispel Tribe of Indians (Tribe) purchased three projects totaling nearly 1,200 acres. The Tacoma/Trimble Wildlife Management Area is a conglomeration of properties now estimated at 1,700 acres. It is the Tribe's intent to manage these properties in cooperation and collaboration with the Pend Oreille County Public Utility District (PUD) No. 1 and the U.S. Fish and Wildlife Service (USFWS) to benefit wildlife habitats and associated species, populations,more » and guilds.« less
Reliability Quantification of Advanced Stirling Convertor (ASC) Components

NASA Technical Reports Server (NTRS)

Shah, Ashwin R.; Korovaichuk, Igor; Zampino, Edward

2010-01-01

The Advanced Stirling Convertor, is intended to provide power for an unmanned planetary spacecraft and has an operational life requirement of 17 years. Over this 17 year mission, the ASC must provide power with desired performance and efficiency and require no corrective maintenance. Reliability demonstration testing for the ASC was found to be very limited due to schedule and resource constraints. Reliability demonstration must involve the application of analysis, system and component level testing, and simulation models, taken collectively. Therefore, computer simulation with limited test data verification is a viable approach to assess the reliability of ASC components. This approach is based on physics-of-failure mechanisms and involves the relationship among the design variables based on physics, mechanics, material behavior models, interaction of different components and their respective disciplines such as structures, materials, fluid, thermal, mechanical, electrical, etc. In addition, these models are based on the available test data, which can be updated, and analysis refined as more data and information becomes available. The failure mechanisms and causes of failure are included in the analysis, especially in light of the new information, in order to develop guidelines to improve design reliability and better operating controls to reduce the probability of failure. Quantified reliability assessment based on fundamental physical behavior of components and their relationship with other components has demonstrated itself to be a superior technique to conventional reliability approaches based on utilizing failure rates derived from similar equipment or simply expert judgment.
ASC Addresses Unit Commanders’ Concerns through LBE and Reset Programs

DTIC Science & Technology

2008-09-01

Distribution Management Center (DMC). The DMC, based at ASC Headquarters on Rock Island Arsenal, Ilinois, has become the single ASC integrator for LBE and field-level reset in support of ARFORGEN. The reset of units returning from OEF/OIF consists of a series of actions to restore the units to a desired level of combat capability commensurate with future mission requirements. These actions include the repair of equipment, replacement of equipment lost during operations, and recapitalization of equipment where feasible and

Overall accuracy of cervical cytology and clinicopathological significance of LSIL cells in ASC-H cytology.

PubMed

Kim, S H; Lee, J M; Yun, H G; Park, U S; Hwang, S U; Pyo, J-S; Sohn, J H

2017-02-01

The aims of this study were (i) to investigate the diagnostic accuracy of Papanicolaou (Pap) smears and (ii) to evaluate the clinicopathological significance of the presence of low-grade squamous intraepithelial lesion (LSIL) cells in atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion (HSIL) (ASC-H) cytology. We retrospectively reviewed paired cytological and histological findings from 3141 patients. ASC-H cytology was classified as either ASC-H or LSIL with some features suggestive of the presence of a concurrent HSIL (LSIL-H). Clinicopathological characteristics were evaluated through a retrospective study and meta-analysis. The accuracy of the cytological diagnosis was 93.7% (2942 of 3141 cases). The positive predictive value (PPV) of ASC-H for cervical intraepithelial neoplasia grade 2 or worse (CIN 2+ ) was 51.4%. In cases of LSIL-H, CIN 2+ histology was more prevalent in the pre-menopausal period (19-44 years) than in peri- and postmenopausal periods (older than 45 years) (P = 0.024). There was no difference in the ability of LSIL-H and ASC-H to predict CIN 2+. The Pap smear is a good cervical cancer screening method. Although there was no difference in the predictive value for CIN 2+ between LSIL-H and ASC-H, the presence of definite LSIL cells was more predictive of CIN 2+ in younger patients than in older patients. © 2016 John Wiley & Sons Ltd.
Acute stress among adolescents and female rape victims measured by ASC-Kids: a pilot study.

PubMed

Nilsson, Doris; Nordenstam, Carin; Green, Sara; Wetterhall, Annika; Lundin, Tom; Svedin, Carl Göran

2015-01-01

Rape is considered a stressful trauma and often with durable consequences. How the aftermath of rape is for young adolescents' girls considering acute stress is an overlooked field and remains to be studied. In this study, we wanted to investigate acute stress among adolescent victims of rape and the psychometric properties of the Acute Stress Checklist for Children (ASC-Kids). A clinical sample (n = 79) of raped girls, 13-17 years old who had turned to a special rape victim unit for treatment, answered the ASC-Kids. ASC-Kids was also given to a group of minor stressed, non-raped adolescents in the same age range (n = 154) together with the University of California at Los Angeles Post-traumatic Stress Disorder Reaction Index (UCLA PTSD RI), and the Sense of Coherence Scale 13 (SOC-13). The scores from the groups were compared and showed significant differences in mean values on all the diagnostic criteria of acute stress disorder. In the clinical group, 36.7% obtained full ASD criteria. ASC-Kids could discriminate well between groups. Cronbach's alpha was found to be excellent, and the correlation between the UCLA PTSD RI and ASC-Kids found to be good; both ASC-Kids and UCLA PTSD RI had a good and moderate negative correlation with SOC-13. Adolescent female rape victims were shown to have a very high level of acute stress, and the ASC-Kids was found to have sound psychometrics and can be a valuable screening instrument to support clinicians in their assessments of an indication of adolescents after potentially stressful events such as rape.
How many records should be used in ASCE/SEI-7 ground motion scaling procedure?

USGS Publications Warehouse

Reyes, Juan C.; Kalkan, Erol

2012-01-01

U.S. national building codes refer to the ASCE/SEI-7 provisions for selecting and scaling ground motions for use in nonlinear response history analysis of structures. Because the limiting values for the number of records in the ASCE/SEI-7 are based on engineering experience, this study examines the required number of records statistically, such that the scaled records provide accurate, efficient, and consistent estimates of “true” structural responses. Based on elastic–perfectly plastic and bilinear single-degree-of-freedom systems, the ASCE/SEI-7 scaling procedure is applied to 480 sets of ground motions; the number of records in these sets varies from three to ten. As compared to benchmark responses, it is demonstrated that the ASCE/SEI-7 scaling procedure is conservative if fewer than seven ground motions are employed. Utilizing seven or more randomly selected records provides more accurate estimate of the responses. Selecting records based on their spectral shape and design spectral acceleration increases the accuracy and efficiency of the procedure.
Usage patterns and 1-year outcomes with the TAXUS Liberté stent: results of the TAXUS OLYMPIA registry.

PubMed

Ahmed, Waqar H; Mendiz, Oscar A; Thomas, Martyn R

2011-06-01

The TAXUS OLYMPIA registry is a prospective, global, post-approval program designed to collect clinical outcome data through 1 year from patients receiving the TAXUS Liberté paclitaxel-eluting stent in routine interventional cardiology practice. The thin-strut TAXUS Liberté stent has been studied in ongoing clinical trials with specific inclusion/exclusion criteria. Between September 2005 and April 2007, a total of 21,954 patients from 365 sites in 57 countries eligible to receive a TAXUS Liberté stent were enrolled in the TAXUS OLYMPIA registry. Baseline characteristics and procedure patterns were collected and clinical follow-up is available for 1 year. The primary endpoint was the composite cardiac event (cardiac death, MI, and reintervention of the target vessel) rate related to the TAXUS Liberté stent at 1 year. All cardiac events were monitored and all endpoints were independently adjudicated. Complex patients and lesions were prevalent, including: 27% medically-treated diabetes, 58% ACC/AHA type B2/C lesions, 32% multiple stenting, 13% long lesions (>28 mm), and 10% small vessels (<2.5 mm). At 1 year, the composite cardiac event rate was 4.4%, including 1.4% cardiac death, 1.0% MI, and 3.2% TVR. Stent thrombosis (ST, angiographically confirmed) occurred in 0.8% of patients, with 0.4% ST occurring >30 days postprocedure. The composite cardiac event rate related to the TAXUS Liberté stent was 3.8% at 1 year. Low 1-year cardiac event rates were reported with TAXUS Liberté in a broad spectrum of patients, thereby confirming the technical and clinical performance of this stent in a "real-world" setting. Copyright © 2010 Wiley-Liss, Inc.
Validation of Organics for Advanced Stirling Convertor (ASC)

NASA Astrophysics Data System (ADS)

Shin, E. Eugene; Scheiman, Dan; Cybulski, Michelle; Quade, Derek; Inghram, Linda; Burke, Chris

2008-01-01

Organic materials are an essential part of the Advanced Stirling Convertor (ASC) construction as adhesives, potting, wire insulation, lubrication coatings, bobbins, bumpers, insulators, thread lockers. Since a long lifetime of such convertors to be used in the Advanced Stirling Radioisotope Generator (ASRG), sometimes up to 17 years, is required in various space applications such as Mars rovers, deep space missions, and lunar surface power, performance, durability and reliability of those organics should be critically evaluated in every possible material-process-fabrication-service environment relations. The objective of this study was to evaluate, validate, and recommend organics for use in ASCs. Systematic and extensive evaluation methodologies were developed and conducted for various organic materials. The overall efforts dealing with organics materials for the last several years are summarized in the key areas, e.g., process-fabrication optimization, adhesive bonding integrity, outgassing, thermal stability, and durability
Purple L1 Milestone Review Panel TotalView Debugger Functionality and Performance for ASC Purple

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wolfe, M

2006-12-12

ASC code teams require a robust software debugging tool to help developers quickly find bugs in their codes and get their codes running. Development debugging commonly runs up to 512 processes. Production jobs run up to full ASC Purple scale, and at times require introspection while running. Developers want a debugger that runs on all their development and production platforms and that works with all compilers and runtimes used with ASC codes. The TotalView Multiprocess Debugger made by Etnus was specified for ASC Purple to address this needed capability. The ASC Purple environment builds on the environment seen by TotalViewmore » on ASCI White. The debugger must now operate with the Power5 CPU, Federation switch, AIX 5.3 operating system including large pages, IBM compilers 7 and 9, POE 4.2 parallel environment, and rs6000 SLURM resource manager. Users require robust, basic debugger functionality with acceptable performance at development debugging scale. A TotalView installation must be provided at the beginning of the early user access period that meets these requirements. A functional enhancement, fast conditional data watchpoints, and a scalability enhancement, capability up to 8192 processes, are to be demonstrated.« less
VizieR Online Data Catalog: ASC Gaia Attitude Star Catalog (Smart, 2015)

NASA Astrophysics Data System (ADS)

Smart, R. L.

2015-04-01

The ASC is a compilation produced for the Gaia mission. We have combined data from the following catalogs or datasets to produce a homogenous list of positions, proper motions, photometry in a blue and red band and estimates of the magnitudes in the Gaia G and G_RVS bands: Tycho2, UCAC4, Hipparcos, PPMXL, GSC2.3 and Sky2000. Originally ASC sources were selected from the Initial Gaia Source List (IGSL, I/324). However, here we produce a cleaner catalog starting from the bright source catalogs and using the following criteria: 1) The candidate must be in the Tycho2, UCAC4, Hipparcos or Sky2000 catalog. 2) The Gaia G magnitude must be brighter than 13.4. 3) The star must be isolated from other objects of similar magnitudes 4) The object must not be in the Washington Double Star catalog 5) If a healpix 6th region has more than 1000 objects the magnitude limit is reduced to reduce the number of objects in that region. Since the ASC was produced independently from the IGSL using different procedures there is not a direct 1 to 1 match between ASC and IGSL entries. We have matched the ASC to the IGSL and found that 9 out of the 8 million entries do not have a clear match. Since there may still remain ambiguous matches in the 8 million matched objects, we decided to assign the sourceIDs of the IGSL with the adjustment that the runningnumber is equal to the IGSL runningnumber + 320000. Included Catalogs: Tycho2, UCAC4, Sky2000, HIPPARCOS for candidates and the PPMXL, GSC2.3 were used to calculating magnitudes. (2 data files).
Nlrp6- and ASC-Dependent Inflammasomes Do Not Shape the Commensal Gut Microbiota Composition.

PubMed

Mamantopoulos, Michail; Ronchi, Francesca; Van Hauwermeiren, Filip; Vieira-Silva, Sara; Yilmaz, Bahtiyar; Martens, Liesbet; Saeys, Yvan; Drexler, Stefan K; Yazdi, Amir S; Raes, Jeroen; Lamkanfi, Mohamed; McCoy, Kathy D; Wullaert, Andy

2017-08-15

The gut microbiota regulate susceptibility to multiple human diseases. The Nlrp6-ASC inflammasome is widely regarded as a hallmark host innate immune axis that shapes the gut microbiota composition. This notion stems from studies reporting dysbiosis in mice lacking these inflammasome components when compared with non-littermate wild-type animals. Here, we describe microbial analyses in inflammasome-deficient mice while minimizing non-genetic confounders using littermate-controlled Nlrp6-deficient mice and ex-germ-free littermate-controlled ASC-deficient mice that were all allowed to shape their gut microbiota naturally after birth. Careful microbial phylogenetic analyses of these cohorts failed to reveal regulation of the gut microbiota composition by the Nlrp6- and ASC-dependent inflammasomes. Our results obtained in two geographically separated animal facilities dismiss a generalizable impact of Nlrp6- and ASC-dependent inflammasomes on the composition of the commensal gut microbiota and highlight the necessity for littermate-controlled experimental design in assessing the influence of host immunity on gut microbial ecology. Copyright © 2017 Elsevier Inc. All rights reserved.
75 FR 65516 - ASC Machine Tools, Inc., Spokane Valley, WA; Notice of Affirmative Determination Regarding...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-25

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-72,971] ASC Machine Tools, Inc... workers and former workers of ASC Machine Tools, Inc., Spokane Valley, Washington (the subject firm). The... cut metal, including assembled equipment, component parts of equipment, and spare parts. The negative...
Denman Island disease in Washington State, USA: distribution and prevalence in Pacific and Olympia oysters.

PubMed

Elston, Ralph; Friedman, Carolyn; Gustafson, Lori; Meyer, Gary; Rogers, Russell

2015-05-21

We sampled over 2400 wild, feral, and cultured Pacific oysters Crassostrea gigas and Olympia oysters Ostrea lurida in Washington State, USA, from 2002 to 2006 to estimate the prevalence of infection with Mikrocytos mackini, the causative agent of Denman Island disease. Both histology and qualitative PCR methods were used. Estimates of true prevalence of M. mackini infection in C. gigas, after accounting for imperfect test sensitivity, ranged from mean values of 0 to 10.0% by histology and 0 to 8.4% based on pooled PCR samples. M. mackini was not detected in any of the O. lurida samples. Results suggest a lower prevalence of the pathogen and severity of this oyster disease in Washington than that indicated in previous reports from British Columbia, Canada, potentially attributable to higher seawater temperatures in the Washington sample locations.
The ASC/SIL ratio for cytopathologists as a quality control measure: a follow-up study.

PubMed

Nascimento, Alessandra F; Cibas, Edmund S

2007-10-01

Monitoring the relative frequency of the interpretations of atypical squamous cells (ASC) and squamous intraepithelial lesions (SIL) has been proposed as a quality control measure. To assess its value, an ASC/SIL ratio was calculated every 6 months for 3.5 years, and confidential feedback was provided to 10 cytopathologists (CPs). By using simple regression analysis, we analyzed the initial and final ASC/SIL ratios for individual CPs and for the entire group. The ratio was below the upper benchmark of 3:1 for all but 1 CP during every 6-month period. The ratio for all CPs combined showed a downward trend (from 2.05 to 1.73). The ratio for 6 CPs decreased, and for two of them the decrease was statistically significant. One CP showed a statistically significant increase in the ASC/SIL ratio. The decrease for some CPs likely reflects the salutary effect of confidential feedback and counseling.
Antiproliferative effect of ASC-J9 delivered by PLGA nanoparticles against estrogen-dependent breast cancer cells.

PubMed

Verderio, Paolo; Pandolfi, Laura; Mazzucchelli, Serena; Marinozzi, Maria Rosaria; Vanna, Renzo; Gramatica, Furio; Corsi, Fabio; Colombo, Miriam; Morasso, Carlo; Prosperi, Davide

2014-08-04

Among polymeric nanoparticles designed for cancer therapy, PLGA nanoparticles have become one of the most popular polymeric devices for chemotherapeutic-based nanoformulations against several kinds of malignant diseases. Promising properties, including long-circulation time, enhanced tumor localization, interference with "multidrug" resistance effects, and environmental biodegradability, often result in an improvement of the drug bioavailability and effectiveness. In the present work, we have synthesized 1,7-bis(3,4-dimethoxyphenyl)-5-hydroxyhepta-1,4,6-trien-3-one (ASC-J9) and developed uniform ASC-J9-loaded PLGA nanoparticles of about 120 nm, which have been prepared by a single-emulsion process. Structural and morphological features of the nanoformulation were analyzed, followed by an accurate evaluation of the in vitro drug release kinetics, which exhibited Fickian law diffusion over 10 days. The intracellular degradation of ASC-J9-bearing nanoparticles within estrogen-dependent MCF-7 breast cancer cells was correlated to a time- and dose-dependent activity of the released drug. A cellular growth inhibition associated with a specific cell cycle G2/M blocking effect caused by ASC-J9 release inside the cytosol allowed us to put forward a hypothesis on the action mechanism of this nanosystem, which led to the final cell apoptosis. Our study was accomplished using Annexin V-based cell death analysis, MTT assessment of proliferation, radical scavenging activity, and intracellular ROS evaluation. Moreover, the intracellular localization of nanoformulated ASC-J9 was confirmed by a Raman optical imaging experiment designed ad hoc. PLGA nanoparticles and ASC-J9 proved also to be safe for a healthy embryo fibroblast cell line (3T3-L1), suggesting a possible clinical translation of this potential nanochemotherapeutic to expand the inherently poor bioavailability of hydrophobic ASC-J9 that could be proposed for the treatment of malignant breast cancer.
77 FR 39657 - Approval and Promulgation of Air Quality Implementation Plans; Washington; Determination of Clean...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-05

... Fine Particulate Standard for the Tacoma, Pierce County Nonattainment Area AGENCY: Environmental..., Pierce County nonattainment area (hereafter referred to as ``Tacoma, Pierce County'' or ``the area'') for... section 107(d)(1), for the 2006 24-hour PM 2.5 NAAQS. As part of that action, Tacoma, Pierce County...
Environmental Loss Characterization of an Advanced Stirling Convertor (ASC-E2) Insulation Package Using a Mock Heater Head

NASA Technical Reports Server (NTRS)

Schifer, Nicholas A.; Briggs, Maxwell H.

2012-01-01

The U.S. Department of Energy (DOE) and Lockheed Martin Space Systems Company (LMSSC) have been developing the Advanced Stirling Radioisotope Generator (ASRG) for use as a power system for space science missions. This generator would use two highefficiency Advanced Stirling Convertors (ASCs), developed by Sunpower Inc. and NASA Glenn Research Center (GRC). As part of ground testing of these ASCs, different operating conditions are used to simulate expected mission conditions. These conditions require achieving a specified electrical power output for a given net heat input. While electrical power output can be precisely quantified, thermal power input to the Stirling cycle cannot be directly measured. In an effort to improve net heat input predictions, the Mock Heater Head was developed with the same relative thermal paths as a convertor using a conducting rod to represent the Stirling cycle and tested to provide a direct comparison to numerical and empirical models used to predict convertor net heat input. The Mock Heater Head also served as the pathfinder for a higher fidelity version of validation test hardware, known as the Thermal Standard. This paper describes how the Mock Heater Head was tested and utilized to validate a process for the Thermal Standard.
Coast-wide recruitment dynamics of Olympia oysters reveal limited synchrony and multiple predictors of failure.

PubMed

Wasson, Kerstin; Hughes, Brent B; Berriman, John S; Chang, Andrew L; Deck, Anna K; Dinnel, Paul A; Endris, Charlie; Espinoza, Michael; Dudas, Sarah; Ferner, Matthew C; Grosholz, Edwin D; Kimbro, David; Ruesink, Jennifer L; Trimble, Alan C; Vander Schaaf, Dick; Zabin, Chela J; Zacherl, Danielle C

2016-12-01

Recruitment of new propagules into a population can be a critical determinant of adult density. We examined recruitment dynamics in the Olympia oyster (Ostrea lurida), a species occurring almost entirely in estuaries. We investigated spatial scales of interannual synchrony across 37 sites in eight estuaries along 2,500 km of Pacific North American coastline, predicting that high vs. low recruitment years would coincide among neighboring estuaries due to shared exposure to regional oceanographic factors. Such synchrony in recruitment has been found for many marine species and some migratory estuarine species, but has never been examined across estuaries in a species that can complete its entire life cycle within the same estuary. To inform ongoing restoration efforts for Olympia oysters, which have declined in abundance in many estuaries, we also investigated predictors of recruitment failure. We found striking contrasts in absolute recruitment rate and frequency of recruitment failure among sites, estuaries, and years. Although we found a positive relationship between upwelling and recruitment, there was little evidence of synchrony in recruitment among estuaries along the coast, and only limited synchrony of sites within estuaries, suggesting recruitment rates are affected more strongly by local dynamics within estuaries than by regional oceanographic factors operating at scales encompassing multiple estuaries. This highlights the importance of local wetland and watershed management for the demography of oysters, and perhaps other species that can complete their entire life cycle within estuaries. Estuaries with more homogeneous environmental conditions had greater synchrony among sites, and this led to the potential for estuary-wide failure when all sites had no recruitment in the same year. Environmental heterogeneity within estuaries may thus buffer against estuary-wide recruitment failure, analogous to the portfolio effect for diversity. Recruitment failure
7 CFR 1945.28 - Relationship between ASCS and FmHA or its successor agency under Public Law 103-354.

Code of Federal Regulations, 2010 CFR

2010-01-01

... intended to assist in maintaining and improving the working relationship between the ASCS and the FmHA or... 7 Agriculture 13 2010-01-01 2009-01-01 true Relationship between ASCS and FmHA or its successor...) EMERGENCY Disaster Assistance-General § 1945.28 Relationship between ASCS and FmHA or its successor agency...
Hypoxia triggers angiogenesis by increasing expression of LOX genes in 3-D culture of ASCs and ECs.

PubMed

Xie, Qiang; Xie, Jiamin; Tian, Taoran; Ma, Quanquan; Zhang, Qi; Zhu, Bofeng; Cai, Xiaoxiao

2017-03-01

This study aimed to investigate the expression changes of LOX (lysyl oxidase) family genes, VEGFA, and VEGFB under hypoxic conditions in a co-culture system of ASCs (adipose-derived stromal cells) and ECs (endothelial cells). ASCs and ECs were co-cultured under hypoxic and normal oxygen conditions in a 1:1 ratio, and the formation of vessel-like was detected at 7 days. The transwell co-culture system was used and cell lysates were collected at 7 days after co-culture in hypoxic and normal oxygen condition. Semi-quantitative PCR was performed to quantify the mRNA expression of VEGFA, VEGFB, and the LOX genes (LOX, LOXL-1, LOXL-2, LOXL-3, and LOXL-4). Expression changes were determined by densitomery. Enhanced angiogenesis was detected in the co-culture of ASCs and ECs under hypoxic conditions. Among the genes screened, VEGFA, VEGFB, LOXL-1, and LOXL-3 in ECs, both mono-cultured and co-cultured, were significantly enhanced after culturing under hypoxic conditions. In ASCs, VEGFB, LOXL-1, and LOXL-3 were upregulated. Contact co-culture between ASCs and ECs promotes angiogenesis under hypoxia. LOXL-1 and LOXL-3 expressions were increased in both ASCs and ECs and might play important roles in the enhanced angiogenesis promoted by hypoxia. Copyright © 2017 Elsevier Inc. All rights reserved.
In Vivo d-Serine Hetero-Exchange through Alanine-Serine-Cysteine (ASC) Transporters Detected by Microelectrode Biosensors

PubMed Central

2013-01-01

d-Serine, a co-agonist of N-methyl d-aspartate (NMDA) receptors, has been implicated in neurological and psychiatric disorders such as cerebral ischemia, lateral amyotrophic sclerosis, or schizophrenia. d-Serine signaling represents an important pharmacological target for treating these diseases; however, the biochemical mechanisms controlling extracellular d-serine levels in vivo are still unclear. d-Serine heteroexchange through small neutral amino acid transporters has been shown in cell cultures and brain slices and could provide a biochemical mechanism for the control of d-serine extracellular concentration in vivo. Alternatively, exocytotic d-serine release has also been proposed. In this study, the dynamics of d-serine release and clearance were explored in vivo on a second-by-second time scale using microelectrode biosensors. The rate of d-serine clearance in the rat frontal cortex after a microionophoretic injection revealed a transporter-mediated uptake mechanism. d-Serine uptake was blocked by small neutral l-amino acids, implicating alanine-serine-cysteine (ASC) transporters, in particular high affinity Asc-1 and low affinity ASCT2 transporters. Interestingly, changes in alanine, serine, or threonine levels resulted in d-serine release through ASC transporters. Asc-1, but not ASCT2, appeared to release d-serine in response to changes in amino acid concentrations. Finally, neuronal silencing by tetrodotoxin increased d-serine extracellular concentration by an ASC-transporter-dependent mechanism. Together, these results indicate that d-serine heteroexchange through ASC transporters is present in vivo and may constitute a key component in the regulation of d-serine extracellular concentration. PMID:23581544
New therapy with ASC-J9® to suppress the prostatitis via altering the cytokine CCL2 signals

PubMed Central

Lin, Shin-Jen; Chou, Fu-Ju; Lin, Chang-Yi; Chang, Hong-Chiang; Yeh, Shuyuan; Chang, Chawnshang

2016-01-01

Prostatitis is a common disease contributing to 8% of all urologist visits. Yet the etiology and effective treatment remain to be further elucidated. Using a non-obese diabetes mouse model that can be induced by autoimmune response for the spontaneous development of prostatitis, we found that injection of the ASC-J9® at 75 mg/Kg body weight/48 hours led to significantly suppressed prostatitis that was accompanied with reduction of lymphocyte infiltration with reduced CD4+ T cells in prostate. In vitro studies with a co-culture system also confirmed that ASC-J9® treatment could suppress the CD4+ T cell migration to prostate stromal cells. Mechanisms dissection indicated that ASC-J9® can suppress CD4+ T cell migration via decreasing the cytokine CCL2 in vitro and in vivo, and restoring CCL2 could interrupt the ASC-J9® suppressed CD4+ T cell migration. Together, results from in vivo and in vitro studies suggest that ASC-J9® can suppress prostatitis by altering the autoimmune response induced by CD4+ T cell recruitment, and using ASC-J9® may help us to develop a potential new therapy to battle the prostatitis with little side effects. PMID:27564257
New therapy with ASC-J9® to suppress the prostatitis via altering the cytokine CCL2 signals.

PubMed

Lin, Shin-Jen; Chou, Fu-Ju; Lin, Chang-Yi; Chang, Hong-Chiang; Yeh, Shuyuan; Chang, Chawnshang

2016-10-11

Prostatitis is a common disease contributing to 8% of all urologist visits. Yet the etiology and effective treatment remain to be further elucidated. Using a non-obese diabetes mouse model that can be induced by autoimmune response for the spontaneous development of prostatitis, we found that injection of the ASC-J9® at 75 mg/Kg body weight/48 hours led to significantly suppressed prostatitis that was accompanied with reduction of lymphocyte infiltration with reduced CD4+ T cells in prostate. In vitro studies with a co-culture system also confirmed that ASC-J9® treatment could suppress the CD4+ T cell migration to prostate stromal cells. Mechanisms dissection indicated that ASC-J9® can suppress CD4+ T cell migration via decreasing the cytokine CCL2 in vitro and in vivo, and restoring CCL2 could interrupt the ASC-J9® suppressed CD4+ T cell migration. Together, results from in vivo and in vitro studies suggest that ASC-J9® can suppress prostatitis by altering the autoimmune response induced by CD4+ T cell recruitment, and using ASC-J9® may help us to develop a potential new therapy to battle the prostatitis with little side effects.

The American Satellite Company (ASC) satellite deployed from payload bay

NASA Technical Reports Server (NTRS)

1985-01-01

The American Satellite Company (ASC) communications satellite is deployed from the payload bay of the Shuttle Discovery. A portion of the cloudy surface of the earth can be seen to the left of the frame.
FY17 ASC CSSE L2 Milestone 6018: Power Usage Characteristics of Workloads Running on Trinity.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pedretti, Kevin

The overall goal of this work was to utilize the Advanced Power Management (APM) capabilities of the ATS-1 Trinity platform to understand the power usage behavior of ASC workloads running on Trinity and gain insight into the potential for utilizing power management techniques on future ASC platforms.
Human adipose stem cell and ASC-derived cardiac progenitor cellular therapy improves outcomes in a murine model of myocardial infarction

PubMed Central

Davy, Philip MC; Lye, Kevin D; Mathews, Juanita; Owens, Jesse B; Chow, Alice Y; Wong, Livingston; Moisyadi, Stefan; Allsopp, Richard C

2015-01-01

Background Adipose tissue is an abundant and potent source of adult stem cells for transplant therapy. In this study, we present our findings on the potential application of adipose-derived stem cells (ASCs) as well as induced cardiac-like progenitors (iCPs) derived from ASCs for the treatment of myocardial infarction. Methods and results Human bone marrow (BM)-derived stem cells, ASCs, and iCPs generated from ASCs using three defined cardiac lineage transcription factors were assessed in an immune-compromised mouse myocardial infarction model. Analysis of iCP prior to transplant confirmed changes in gene and protein expression consistent with a cardiac phenotype. Endpoint analysis was performed 1 month posttransplant. Significantly increased endpoint fractional shortening, as well as reduction in the infarct area at risk, was observed in recipients of iCPs as compared to the other recipient cohorts. Both recipients of iCPs and ASCs presented higher myocardial capillary densities than either recipients of BM-derived stem cells or the control cohort. Furthermore, mice receiving iCPs had a significantly higher cardiac retention of transplanted cells than all other groups. Conclusion Overall, iCPs generated from ASCs outperform BM-derived stem cells and ASCs in facilitating recovery from induced myocardial infarction in mice. PMID:26604802
Overview of ASC Capability Computing System Governance Model

DOE Office of Scientific and Technical Information (OSTI.GOV)

Doebling, Scott W.

This document contains a description of the Advanced Simulation and Computing Program's Capability Computing System Governance Model. Objectives of the Governance Model are to ensure that the capability system resources are allocated on a priority-driven basis according to the Program requirements; and to utilize ASC Capability Systems for the large capability jobs for which they were designed and procured.
Probability-Based Design Criteria of the ASCE 7 Tsunami Loads and Effects Provisions (Invited)

NASA Astrophysics Data System (ADS)

Chock, G.

2013-12-01

Mitigation of tsunami risk requires a combination of emergency preparedness for evacuation in addition to providing structural resilience of critical facilities, infrastructure, and key resources necessary for immediate response and economic and social recovery. Critical facilities would include emergency response, medical, tsunami refuges and shelters, ports and harbors, lifelines, transportation, telecommunications, power, financial institutions, and major industrial/commercial facilities. The Tsunami Loads and Effects Subcommittee of the ASCE/SEI 7 Standards Committee is developing a proposed new Chapter 6 - Tsunami Loads and Effects for the 2016 edition of the ASCE 7 Standard. ASCE 7 provides the minimum design loads and requirements for structures subject to building codes such as the International Building Code utilized in the USA. In this paper we will provide a review emphasizing the intent of these new code provisions and explain the design methodology. The ASCE 7 provisions for Tsunami Loads and Effects enables a set of analysis and design methodologies that are consistent with performance-based engineering based on probabilistic criteria. . The ASCE 7 Tsunami Loads and Effects chapter will be initially applicable only to the states of Alaska, Washington, Oregon, California, and Hawaii. Ground shaking effects and subsidence from a preceding local offshore Maximum Considered Earthquake will also be considered prior to tsunami arrival for Alaska and states in the Pacific Northwest regions governed by nearby offshore subduction earthquakes. For national tsunami design provisions to achieve a consistent reliability standard of structural performance for community resilience, a new generation of tsunami inundation hazard maps for design is required. The lesson of recent tsunami is that historical records alone do not provide a sufficient measure of the potential heights of future tsunamis. Engineering design must consider the occurrence of events greater than
Brief Report: Attentional Cueing to Images of Social Interactions is Automatic for Neurotypical Individuals But Not Those with ASC.

PubMed

Morrisey, Marcus Neil; Reed, Catherine L; McIntosh, Daniel N; Rutherford, M D

2018-04-25

Human actions induce attentional orienting toward the target of the action. We examined the influence of action cueing in social (man throwing toward a human) and non-social (man throwing toward a tree) contexts in observers with and without autism spectrum condition (ASC). Results suggested that a social interaction enhanced the cueing effect for neurotypical participants. Participants with ASC did not benefit from non-predictive cues and were slower in social contexts, although they benefitted from reliably predictive cues. Social orienting appears to be automatic in the context of an implied social interaction for neurotypical observers, but not those with ASC. Neurotypical participants' behavior may be driven by automatic processing, while participants with ASC use an alternative, effortful strategy.
Effects of Autologous Fat and ASCs on Swine Hypertrophic Burn Scars: A Multimodal Quantitative Analysis

PubMed Central

Pan, Brian S.; Schwentker, Ann R.; Van Aalst, John

2017-01-01

Background: Hypertrophic scar formation is unpredictable and poorly understood, afflicting both the pediatric and adult populations. Treatment methods with conservative and invasive approaches have low rates of compliance and high rates of morbidity. The purpose of this study was to test a reproducible scar model and investigate a new technique of scar modification through the use of adipose- derived progenitor stromal cells (ASCs). Methods: Twenty thermal deep-partial thickness contact burns were created on the dorsum of three 8-week-old domestic swine and allowed to mature for 10 weeks. Scars were then injected with 2 cc saline, expanded autologous ASCs, or 2 cc fresh lipoaspirate and sampled at 2 week intervals up to 10 weeks postinjection. Volumetric analysis with a 3-D scanner, mechanical elasticity testing through negative pressure transduction, and standardized photography evaluation with Image J was performed. RNA sequencing was performed on scar tissue samples, cultured cells, and fresh lipoaspirate to determine relevant gene transcription regulation. Immunohistochemistry was used to verify expression level changes within the scars. Results: Volumetric analysis demonstrates a reduction in average scar thickness at 6 weeks when injected with ASCs (−1.6 cc3) and autologous fat (−1.95 cc3) relative to controls (−0.121 cc3; P < 0.05). A decrease in overall tissue compliance is observed with fat or ASC injection when compared with unburned skin at 8 weeks (35.99/37.94 versus 49.36 mm Hg × mm; P < 0.01). RNA sequencing demonstrates altered regulation of fibroblast gene expression and a decreased inflammatory profile when scars are injected with autologous fat/ASCs over controls. Conclusion: Early results suggest that autologous fat and/or ASCs may improve healing of hypertrophic scarring by altering the cellular and structural components during wound remodeling up to 20 weeks after injury. This may have beneficial applications in early treatment of
NLRP3 and ASC suppress lupus-like autoimmunity by driving the immunosuppressive effects of TGF-β receptor signalling.

PubMed

Lech, Maciej; Lorenz, Georg; Kulkarni, Onkar P; Grosser, Marian O O; Stigrot, Nora; Darisipudi, Murthy N; Günthner, Roman; Wintergerst, Maximilian W M; Anz, David; Susanti, Heni Eka; Anders, Hans-Joachim

2015-12-01

The NLRP3/ASC inflammasome drives host defence and autoinflammatory disorders by activating caspase-1 to trigger the secretion of mature interleukin (IL)-1β/IL-18, but its potential role in autoimmunity is speculative. We generated and phenotyped Nlrp3-deficient, Asc-deficient, Il-1r-deficient and Il-18-deficient C57BL/6-lpr/lpr mice, the latter being a mild model of spontaneous lupus-like autoimmunity. While lack of IL-1R or IL-18 did not affect the C57BL/6-lpr/lpr phenotype, lack of NLRP3 or ASC triggered massive lymphoproliferation, lung T cell infiltrates and severe proliferative lupus nephritis within 6 months, which were all absent in age-matched C57BL/6-lpr/lpr controls. Lack of NLRP3 or ASC increased dendritic cell and macrophage activation, the expression of numerous proinflammatory mediators, lymphocyte necrosis and the expansion of most T cell and B cell subsets. In contrast, plasma cells and autoantibody production were hardly affected. This unexpected immunosuppressive effect of NLRP3 and ASC may relate to their known role in SMAD2/3 phosphorylation during tumour growth factor (TGF)-β receptor signalling, for example, Nlrp3-deficiency and Asc-deficiency significantly suppressed the expression of numerous TGF-β target genes in C57BL/6-lpr/lpr mice and partially recapitulated the known autoimmune phenotype of Tgf-β1-deficient mice. These data identify a novel non-canonical immunoregulatory function of NLRP3 and ASC in autoimmunity. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/
First quantification of relationship between dune orientation and sediment availability, Olympia Undae, Mars

NASA Astrophysics Data System (ADS)

Fernandez-Cascales, Laura; Lucas, Antoine; Rodriguez, Sébastien; Gao, Xin; Spiga, Aymeric; Narteau, Clément

2018-05-01

Dunes provide unique information about wind regimes on planetary bodies where there is no direct meteorological data. At the eastern margin of Olympia Undae on Mars, dune orientation is measured from satellite imagery and sediment cover is estimated using the high contrast between the dune material and substrate. The analysis of these data provide the first quantification of relationship between sediment availability and dune orientation. Abrupt and smooth dune reorientations are associated with inward and outward dynamics of dunes approaching and ejecting from major sedimentary bodies, respectively. These reorientation patterns along sediment transport pathways are interpreted using a new generation dune model based on the coexistence of two dune growth mechanisms. This model also permits solving of the inverse problem of predicting the wind regime from dune orientation. For bidirectional wind regimes, solutions of this inverse problem show substantial differences in the distributions of sediment flux orientation, which can be attributed to atmospheric flow variations induced by changes in albedo at the boundaries of major dune fields. Then, we conclude that relationships between sediment cover and dune orientation can be used to constrain wind regime and dune field development on Mars and other planetary surfaces.
3D crustal structure and long-period ground motions from a M9.0 megathrust earthquake in the Pacific Northwest region

USGS Publications Warehouse

Olsen, K.B.; Stephenson, W.J.; Geisselmeyer, A.

2008-01-01

We have developed a community velocity model for the Pacific Northwest region from northern California to southern Canada and carried out the first 3D simulation of a Mw 9.0 megathrust earthquake rupturing along the Cascadia subduction zone using a parallel supercomputer. A long-period (<0.5 Hz) source model was designed by mapping the inversion results for the December 26, 2004 Sumatra–Andaman earthquake (Han et al., Science 313(5787):658–662, 2006) onto the Cascadia subduction zone. Representative peak ground velocities for the metropolitan centers of the region include 42 cm/s in the Seattle area and 8–20 cm/s in the Tacoma, Olympia, Vancouver, and Portland areas. Combined with an extended duration of the shaking up to 5 min, these long-period ground motions may inflict significant damage on the built environment, in particular on the highrises in downtown Seattle.
ASC Tri-lab Co-design Level 2 Milestone Report 2015

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hornung, Rich; Jones, Holger; Keasler, Jeff

2015-09-23

In 2015, the three Department of Energy (DOE) National Laboratories that make up the Advanced Sci- enti c Computing (ASC) Program (Sandia, Lawrence Livermore, and Los Alamos) collaboratively explored performance portability programming environments in the context of several ASC co-design proxy applica- tions as part of a tri-lab L2 milestone executed by the co-design teams at each laboratory. The programming environments that were studied included Kokkos (developed at Sandia), RAJA (LLNL), and Legion (Stan- ford University). The proxy apps studied included: miniAero, LULESH, CoMD, Kripke, and SNAP. These programming models and proxy-apps are described herein. Each lab focused on amore » particular combination of abstractions and proxy apps, with the goal of assessing performance portability using those. Performance portability was determined by: a) the ability to run a single application source code on multiple advanced architectures, b) comparing runtime performance between \
Modeling of In-stream Tidal Energy Development and its Potential Effects in Tacoma Narrows, Washington, USA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yang, Zhaoqing; Wang, Taiping; Copping, Andrea E.

Understanding and providing proactive information on the potential for tidal energy projects to cause changes to the physical system and to key water quality constituents in tidal waters is a necessary and cost-effective means to avoid costly regulatory involvement and late stage surprises in the permitting process. This paper presents a modeling study for evaluating the tidal energy extraction and its potential impacts on the marine environment in a real world site - Tacoma Narrows of Puget Sound, Washington State, USA. An unstructured-grid coastal ocean model, fitted with a module that simulates tidal energy devices, was applied to simulate themore » tidal energy extracted by different turbine array configurations and the potential effects of the extraction at local and system-wide scales in Tacoma Narrows and South Puget Sound. Model results demonstrated the advantage of an unstructured-grid model for simulating the far-field effects of tidal energy extraction in a large model domain, as well as assessing the near-field effect using a fine grid resolution near the tidal turbines. The outcome shows that a realistic near-term deployment scenario extracts a very small fraction of the total tidal energy in the system and that system wide environmental effects are not likely; however, near-field effects on the flow field and bed shear stress in the area of tidal turbine farm are more likely. Model results also indicate that from a practical standpoint, hydrodynamic or water quality effects are not likely to be the limiting factor for development of large commercial-scale tidal farms. Results indicate that very high numbers of turbines are required to significantly alter the tidal system; limitations on marine space or other environmental concerns are likely to be reached before reaching these deployment levels. These findings show that important information obtained from numerical modeling can be used to inform regulatory and policy processes for tidal energy
A Magnitude 7.1 Earthquake in the Tacoma Fault Zone-A Plausible Scenario for the Southern Puget Sound Region, Washington

USGS Publications Warehouse

Gomberg, Joan; Sherrod, Brian; Weaver, Craig; Frankel, Art

2010-01-01

The U.S. Geological Survey and cooperating scientists have recently assessed the effects of a magnitude 7.1 earthquake on the Tacoma Fault Zone in Pierce County, Washington. A quake of comparable magnitude struck the southern Puget Sound region about 1,100 years ago, and similar earthquakes are almost certain to occur in the future. The region is now home to hundreds of thousands of people, who would be at risk from the shaking, liquefaction, landsliding, and tsunamis caused by such an earthquake. The modeled effects of this scenario earthquake will help emergency planners and residents of the region prepare for future quakes.
Aeroacoustics research in Europe: The CEAS-ASC report on 2013 highlights

NASA Astrophysics Data System (ADS)

Bennett, G. J.; Kennedy, J.; Meskell, C.; Carley, M.; Jordan, P.; Rice, H.

2015-03-01

The Council of European Aerospace Societies (CEAS) Aeroacoustics Specialists Committee (ASC) supports and promotes the interests of the scientific and industrial aeroacoustics community on an European scale and European aeronautics activities internationally. In this context, "aeroacoustics" encompasses all aerospace acoustics and related areas. Each year the committee highlights some of the research and development projects in Europe. This paper is a report on highlights of aeroacoustics research in Europe in 2013, compiled from information provided to the ASC of the CEAS. During 2013, a number of research programmes involving aeroacoustics were funded by the European Commission. Some of the highlights from these programmes are summarised in this paper, as well as highlights from other programmes funded by national programmes or by industry. Furthermore, a concise summary of the CEAS-ASC workshop "Atmospheric and Ground Effects on Aircraft Noise" held in Seville, Spain in September 2013 is included in this report. Enquiries concerning all contributions should be addressed to the authors who are given at the end of each subsection. This issue of the "highlights" paper is dedicated to the memory of Prof. John A. Fitzpatrick, Professor of Mechanical Engineering, Trinity College Dublin, and a valued member of the Aeroacoustics Specialists Committee. John passed away in September 2012 and is fondly missed across the globe by the friends he made in the Aeroacoustics Community. This paper is edited by PhD graduates and colleagues of John's who conduct research in aeroacoustics, inspired by his thirst for knowledge.
Maintenance of human adipose derived stem cell (hASC) differentiation capabilities using a 3D culture.

PubMed

Lin, Ching-Yu; Huang, Chi-Hui; Wu, Yuan-Kun; Cheng, Nai-Chen; Yu, Jiashing

2014-07-01

In this study, 3D culture system for human adipose-derived stem cell (hASC) using a BioLevitator as the bioreactor for microcarrier-based cultures was established. During the culturing period, hASCs preferred to grow in crevices between microcarriers and a high viability was maintained even when reaching confluency. Adipogenic or osteogenic differential medium was used to induce hASCs and differential potentials of these cells were compared between 2D and 3D environments via RT-PCR and staining quantifications. CEBP/α gene expression was significant higher in 3D condition at day 21 (P < 0.05). Staining quantification indicates that cells cultured in 3D condition have significant better differentiation potential from day 14 to 21 for both adipogenic and osteogenic lineages (P < 0.01).
Aeroacoustics research in Europe: The CEAS-ASC report on 2012 highlights

NASA Astrophysics Data System (ADS)

Bodén, H.; Efraimsson, G.

2013-12-01

The Council of European Aerospace Societies (CEAS) Aeroacoustics Specialists Committee (ASC) supports and promotes the interests of the scientific and industrial aeroacoustics community on an European scale and European aeronautics activities internationally. In this context, "aeroacoustics" encompasses all aerospace acoustics and related areas. Each year the committee highlights some of the research and development projects in Europe. This paper is a report on highlights of aeroacoustics research in Europe in 2012, compiled from information provided to the ASC of the CEAS. During 2012, a number of research programmes involving aeroacoustics were funded by the European Commission. Some of the highlights from these programmes are summarized in this paper, as well as highlights from other programmes funded by national programmes or by industry. Enquiries concerning all contributions should be addressed to the authors who are given at the end of each subsection.
PBO Borehole Strainmeters: 2017 Episodic Tremor and Slip Event for Southern Vancouver Island, BC, Canada through Olympia, WA, USA

NASA Astrophysics Data System (ADS)

Van Boskirk, E. J.; Hodgkinson, K. M.; Gottlieb, M. H.; Johnson, W.; Henderson, D. B.; Mencin, D.; Mattioli, G. S.

2017-12-01

The Plate Boundary Observatory's (PBO) borehole strainmeters along the Cascadia Subduction Zone (CSZ) record the development and migration of Episodic Tremor and Slip (ETS). Along the southern Vancouver Island to Olympia, WA portion of the CSZ ETS events seem to repeat every 14 months. ETS events are non-volcanic tremor swarms that occur over periods of weeks, often migrating along segments of the subduction zone and can release the energy equivalent to a M7 or greater earthquake. Each ETS event is different; initial propagation location, ETS movement, duration, and direction all vary. Constraints provided by strainmeter observations of ETS events illuminate strain release patterns along the subducting slab interface and may help resolve questions regarding the location of the locked zone of the slab and what role ETS events play in the CSZ earthquake cycle. The 2017 CSZ ETS began in early February continuing through early April. Beginning in the northern Olympic Peninsula, near Port Angeles, it migrated south towards Olympia over the course of a week. After a two week pause it resumed under the Straits of Juan de Fuca and propagated northwest under Vancouver Island. There are 15 PBO borehole strainmeters along this segment, and ETS strain observations correlate with seismic and GPS measurements. The PBO borehole strainmeters are sensitive even over great distances from the ETS epicenters, and observe compression or extension relative to the ETS migration. Openly available PBO borehole strainmeter data used by the community has made significant contributions to understanding the ETS process, including the determination that ETS slip is tidally modulated. Data are publically available through UNAVCO and IRIS, which provide links to online tutorials and scripts. There are 32 strainmeters covering the CSZ from southern Vancouver Island, Canada to northern California, USA, and data spans back to 2005. Each site has a Gladwin tensor borehole strainmeter, a Malin three
Profiling Autism Symptomatology: An Exploration of the Q-ASC Parental Report Scale in Capturing Sex Differences in Autism

ERIC Educational Resources Information Center

Ormond, Sarah; Brownlow, Charlotte; Garnett, Michelle Sarah; Rynkiewicz, Agnieszka; Attwood, Tony

2018-01-01

The Questionnaire for Autism Spectrum Conditions (Q-ASC) was developed by Attwood et al. (2011) to identify gender-sensitive profiles of autism symptomatology; prioritise and adjust the direction of clinical interventions; and support positive psychosocial outcomes and prognosis into adulthood. The current research piloted the Q-ASC with parents…
Multiple Functional Domains of Enterococcus faecalis Aggregation Substance Asc10 Contribute to Endocarditis Virulence ▿ †

PubMed Central

Chuang, Olivia N.; Schlievert, Patrick M.; Wells, Carol L.; Manias, Dawn A.; Tripp, Timothy J.; Dunny, Gary M.

2009-01-01

Aggregation substance proteins encoded by sex pheromone plasmids increase the virulence of Enterococcus faecalis in experimental pathogenesis models, including infectious endocarditis models. These large surface proteins may contain multiple functional domains involved in various interactions with other bacterial cells and with the mammalian host. Aggregation substance Asc10, encoded by plasmid pCF10, is induced during growth in the mammalian bloodstream, and pCF10 carriage gives E. faecalis a significant selective advantage in this environment. We employed a rabbit model to investigate the role of various functional domains of Asc10 in endocarditis. The data suggested that the bacterial load of the infected tissue was the best indicator of virulence. Isogenic strains carrying either no plasmid, wild-type pCF10, a pCF10 derivative with an in-frame deletion of the prgB gene encoding Asc10, or pCF10 derivatives expressing other alleles of prgB were examined in this model. Previously identified aggregation domains contributed to the virulence associated with the wild-type protein, and a strain expressing an Asc10 derivative in which glycine residues in two RGD motifs were changed to alanine residues showed the greatest reduction in virulence. Remarkably, this strain and the strain carrying the pCF10 derivative with the in-frame deletion of prgB were both significantly less virulent than an isogenic plasmid-free strain. The data demonstrate that multiple functional domains are important in Asc10-mediated interactions with the host during the course of experimental endocarditis and that in the absence of a functional prgB gene, pCF10 carriage is actually disadvantageous in vivo. PMID:18955479
CpG island methylation of TMS1/ASC and CASP8 genes in cervical cancer

PubMed Central

2009-01-01

Background Gene silencing associated with aberrant methylation of promoter region CpG islands is an acquired epigenetic alteration that serves as an alternative to genetic defects in the inactivation of tumor suppressor and other genes in human cancers. Aims This study describes the methylation status of TMS1/ASC and CASP8 genes in cervical cancer. We also examined the prevalence of TMS1/ASC and CASP8 genes methylation in cervical cancer tissue and none - neo plastic samples in an effort to correlate with smoking habit and clinicopathological features. Method Target DNA was modified by sodium bisulfite, converting all unmethylated, but not methylated, cytosines to uracil, and subsequently amplified by Methylation Specific (MS) PCR with primers specific for methylated versus unmethylated DNA. The PCR product was detected by gel electrophoresis and combined with the clinical records of patients. Results The methylation pattern of the TMS1/ASC and CASP8 genes in specimens of cervical cancer and adjacent normal tissues were detected [5/80 (6.2%), 3/80 (3.75%)-2/80 (2.5%), 1/80 (1.2%) respectively]. No statistical differences were seen in the extent of differentiation, invasion, pathological type and smoking habit between the methylated and unmethylated tissues (P > 0.05). Conclusion The present study conclude that the frequency of TMS1/ASC and CASP8 genes methylation in cervical cancer are rare (< 6%), and have no any critical role in development of cervical cancer. PMID:19258216

18 CFR Appendix 1 to Part 301 - ASC Utility Filing Template

Code of Federal Regulations, 2013 CFR

2013-04-01

... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false ASC Utility Filing Template 1 Appendix 1 to Part 301 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS FOR FEDERAL POWER MARKETING ADMINISTRATIONS AVERAGE SYSTEM COST...
18 CFR Appendix 1 to Part 301 - ASC Utility Filing Template

Code of Federal Regulations, 2010 CFR

2010-04-01

... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false ASC Utility Filing Template 1 Appendix 1 to Part 301 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS FOR FEDERAL POWER MARKETING ADMINISTRATIONS AVERAGE SYSTEM COST...
18 CFR Appendix 1 to Part 301 - ASC Utility Filing Template

Code of Federal Regulations, 2014 CFR

2014-04-01

... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false ASC Utility Filing Template 1 Appendix 1 to Part 301 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS FOR FEDERAL POWER MARKETING ADMINISTRATIONS AVERAGE SYSTEM COST...
18 CFR Appendix 1 to Part 301 - ASC Utility Filing Template

Code of Federal Regulations, 2012 CFR

2012-04-01

... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false ASC Utility Filing Template 1 Appendix 1 to Part 301 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS FOR FEDERAL POWER MARKETING ADMINISTRATIONS AVERAGE SYSTEM COST...
18 CFR Appendix 1 to Part 301 - ASC Utility Filing Template

Code of Federal Regulations, 2011 CFR

2011-04-01

... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false ASC Utility Filing Template 1 Appendix 1 to Part 301 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY REGULATIONS FOR FEDERAL POWER MARKETING ADMINISTRATIONS AVERAGE SYSTEM COST...
Inducible defenses in Olympia oysters in response to an invasive predator.

PubMed

Bible, Jillian M; Griffith, Kaylee R; Sanford, Eric

2017-03-01

The prey naiveté hypothesis suggests that native prey may be vulnerable to introduced predators because they have not evolved appropriate defenses. However, recent evidence suggests that native prey sometimes exhibit induced defenses to introduced predators, as a result of rapid evolution or other processes. We examined whether Olympia oysters (Ostrea lurida) display inducible defenses in the presence of an invasive predator, the Atlantic oyster drill (Urosalpinx cinerea), and whether these responses vary among oyster populations from estuaries with and without this predator. We spawned oysters from six populations distributed among three estuaries in northern California, USA, and raised their offspring through two generations under common conditions to minimize effects of environmental history. We exposed second-generation oysters to cue treatments: drills eating oysters, drills eating barnacles, or control seawater. Oysters from all populations grew smaller shells when exposed to drill cues, and grew thicker and harder shells when those drills were eating oysters. Oysters exposed to drills eating other oysters were subsequently preyed upon at a slower rate. Although all oyster populations exhibited inducible defenses, oysters from the estuary with the greatest exposure to drills grew the smallest shells suggesting that oyster populations have evolved adaptive differences in the strength of their responses to predators. Our findings add to a growing body of literature that suggests that marine prey may be less likely to exhibit naiveté in the face of invasive predators than prey in communities that are more isolated from native predators, such as many freshwater and terrestrial island ecosystems.
The influence of food supply on the response of Olympia oyster larvae to ocean acidification

NASA Astrophysics Data System (ADS)

Hettinger, A.; Sanford, E.; Hill, T. M.; Hosfelt, J. D.; Russell, A. D.; Gaylord, B.

2013-03-01

Increases in atmospheric carbon dioxide drive accompanying changes in the marine carbonate system as carbon dioxide (CO2) enters seawater and alters its pH (termed "ocean acidification"). However, such changes do not occur in isolation, and other environmental factors have the potential to modulate the consequences of altered ocean chemistry. Given that physiological mechanisms used by organisms to confront acidification can be energetically costly, we explored the potential for food supply to influence the response of Olympia oyster (Ostrea lurida) larvae to ocean acidification. In laboratory experiments, we reared oyster larvae under a factorial combination of pCO2 and food level. High food availability offset the negative consequences of elevated pCO2 on larval shell growth and total dry weight. Low food availability, in contrast, exacerbated these impacts. In both cases, effects of food and pCO2 interacted additively rather than synergistically, indicating that they operated independently. Despite the potential for abundant resources to counteract the consequences of ocean acidification, impacts were never completely negated, suggesting that even under conditions of enhanced primary production and elevated food availability, impacts of ocean acidification may still accrue in some consumers.
7 CFR 1945.28 - Relationship between ASCS and FmHA or its successor agency under Public Law 103-354.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 13 2011-01-01 2009-01-01 true Relationship between ASCS and FmHA or its successor...) EMERGENCY Disaster Assistance-General § 1945.28 Relationship between ASCS and FmHA or its successor agency under Public Law 103-354. Exhibit A of FmHA Instruction 2000-JJ (a copy of which is available in any Fm...
An Alternative Optimization Model and Robust Experimental Design for the Assignment Scheduling Capability for Unmanned Aerial Vehicles (ASC-U) Simulation

DTIC Science & Technology

2007-06-01

introduces ASC-U’s approach for solving the dynamic UAV allocation problem. 26 Christopher J...18 Figure 6. Assignments Dynamics Example (after) .........................................................20 Figure 7. ASC-U Dynamic Cueing...decisions in order to respond to the dynamic environment they face. Thus, to succeed, the Army’s transformation cannot rely
Androgen receptor (AR) degradation enhancer ASC-J9® in an FDA-approved formulated solution suppresses castration resistant prostate cancer cell growth.

PubMed

Cheng, Max A; Chou, Fu-Ju; Wang, Keliang; Yang, Rachel; Ding, Jie; Zhang, Qiaoxia; Li, Gonghui; Yeh, Shuyuan; Xu, Defeng; Chang, Chawnshang

2018-03-28

ASC-J9 ® is a recently-developed androgen receptor (AR)-degradation enhancer that effectively suppresses castration resistant prostate cancer (PCa) cell proliferation and invasion. The optimal half maximum inhibitory concentrations (IC 50 ) of ASC-J9 ® at various PCa cell confluences (20%, 50%, and 100%) were assessed via both short-term MTT growth assays and long-term clonogenic proliferation assays. Our results indicate that the IC 50 values for ASC-J9 ® increased with increasing cell confluency. The IC 50 values were significantly decreased in PCa AR-positive cells compared to PCa AR-negative cells or in normal prostate cells. This suggests that ASC-J9 ® may function mainly via targeting the AR-positive PCa cells with limited unwanted side-effects to suppress the surrounding normal prostate cells. Mechanism dissection indicated that ASC-J9 ® might function via altering the apoptosis signals to suppress the PCa AR-negative PC-3 cells. Preclinical studies using multiple in vitro PCa cell lines and an in vivo mouse model with xenografted castration-resistant PCa CWR22Rv1 cells demonstrated that ASC-J9 ® has similar AR degradation effects when dissolved in FDA-approved solvents, including DMSO, PEG-400:Tween-80 (95:5), DMA:Labrasol:Tween-80 (10:45:45), and DMA:Labrasol:Tween-20 (10:45:45). Together, results from preclinical studies suggest a potential new therapy with AR-degradation enhancer ASC-J9 ® may potentially be ready to be used in human clinical trials in order to better suppress PCa at later castration resistant stages. Copyright © 2017 Elsevier B.V. All rights reserved.
EGF and hydrocortisone as critical factors for the co-culture of adipogenic differentiated ASCs and endothelial cells.

PubMed

Volz, Ann-Cathrin; Huber, Birgit; Schwandt, Alina Maria; Kluger, Petra Juliane

In vitro composed vascularized adipose tissue is and will continue to be in great demand e.g. for the treatment of extensive high-graded burns or the replacement of tissue after tumor removal. Up to date, the lack of adequate culture conditions, mainly a culture medium, decelerates further achievements. In our study, we evaluated the influence of epidermal growth factor (EGF) and hydrocortisone (HC), often supplemented in endothelial cell (EC) specific media, on the co-culture of adipogenic differentiated adipose-derived stem cells (ASCs) and microvascular endothelial cells (mvECs). In ASCs, EGF and HC are thought to inhibit adipogenic differentiation and have lipolytic activities. Our results showed that in indirect co-culture for 14 days, adipogenic differentiated ASCs further incorporated lipids and partly gained an univacuolar morphology when kept in media with low levels of EGF and HC. In media with high EGF and HC levels, cells did not incorporate further lipids, on the contrary, cells without lipid droplets appeared. Glycerol release, to measure lipolysis, also increased with elevated amounts of EGF and HC in the culture medium. Adipogenic differentiated ASCs were able to release leptin in all setups. MvECs were functional and expressed the cell specific markers, CD31 and von Willebrand factor (vWF), independent of the EGF and HC content as long as further EC specific factors were present. Taken together, our study demonstrates that adipogenic differentiated ASCs can be successfully co-cultured with mvECs in a culture medium containing low or no amounts of EGF and HC, as long as further endothelial cell and adipocyte specific factors are available. Copyright © 2017 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.
A Study of HPV Typing for the Management of HPV-Positive ASC-US Cervical Cytologic Results

PubMed Central

Schiffman, Mark; Vaughan, Laurence; Raine-Bennett, Tina R.; Castle, Philip E.; Katki, Hormuzd A.; Gage, Julia C.; Fetterman, Barbara; Befano, Brian; Wentzensen, Nicolas

2015-01-01

Background In US cervical screening, immediate colposcopy is recommended for women with HPV-positive ASC-US (equivocal) cytology. We evaluated whether partial typing by Onclarity™ (BD) might identify HPV-positive women with low enough CIN3+ risk to permit 1-year follow-up instead. Methods The NCI-Kaiser Permanente Northern California Persistence and Progression Cohort includes a subset of 13,890 women aged 21+ with HC2 (Qiagen)-positive ASC-US at enrollment; current median follow-up is 3.0 years. Using stratified random sampling, we typed 2,079 archived enrollment specimens including 329 women subsequently diagnosed with CIN3+, 563 with CIN2, and 1,187 with ASC-US was 5.2%; this establishes the “benchmark” risk for colposcopic referral. Hierarchically, 3-year cumulative risks for each typing channel were 16.0% for HPV16, 7.4% for HPV18, 7.0% for HPV31, 7.1% for grouped HPV33/58, 4.4% for HPV52, 3.9% for HPV45, 2.7% for HPV51, 1.6% for HPV39/68/35, and 1.3% for HPV59/56/66. Discussion ASC-US linked to HPV16, HPV18, HPV31, or HPV33/58 warrants immediate colposcopy. Optimal management of women with HPV52 or HPV45 is uncertain. Risk of women with only HPV51, HPV39/68/35, or HPV59/56/66 might be low enough to recommend 1-year retesting permitting viral clearance. This strategy would defer colposcopy for 40% of women with HPV-positive ASC-US, half of whom would be cotest-negative at 1-year return. Approximately 10% of those with CIN3 diagnosable at enrollment would be delayed 1 year instead. Cost-effectiveness analyses are needed. PMID:26148763
A study of HPV typing for the management of HPV-positive ASC-US cervical cytologic results.

PubMed

Schiffman, Mark; Vaughan, Laurence M; Raine-Bennett, Tina R; Castle, Philip E; Katki, Hormuzd A; Gage, Julia C; Fetterman, Barbara; Befano, Brian; Wentzensen, Nicolas

2015-09-01

In US cervical screening, immediate colposcopy is recommended for women with HPV-positive ASC-US (equivocal) cytology. We evaluated whether partial typing by Onclarity™ (BD) might identify HPV-positive women with low enough CIN3+ risk to permit 1-year follow-up instead. The NCI-Kaiser Permanente Northern California Persistence and Progression cohort includes a subset of 13,890 women aged 21+ with HC2 (Qiagen)-positive ASC-US at enrollment; current median follow-up is 3.0years. Using stratified random sampling, we typed 2079 archived enrollment specimens including 329 women subsequently diagnosed with CIN3+, 563 with CIN2, and 1187 with ASC-US was 5.2%; this establishes the "benchmark" risk for colposcopic referral. Hierarchically, 3-year cumulative risks for each typing channel were 16.0% for HPV16, 7.4% for HPV18, 7.0% for HPV31, 7.1% for grouped HPV33/58, 4.3% for HPV52, 3.9% for HPV45, 2.7% for HPV51, 1.6% for HPV39/68/35, and 1.3% for HPV59/56/66. ASC-US linked to HPV16, HPV18, HPV31, or HPV33/58 warrants immediate colposcopy. Optimal management of women with HPV52 or HPV45 is uncertain. Risk of women with only HPV51, HPV39/68/35, or HPV59/56/66 might be low enough to recommend 1-year retesting permitting viral clearance. This strategy would defer colposcopy for 40% of women with HPV-positive ASC-US, half of whom would be cotest-negative at 1-year return. Approximately 10% of those with CIN3 diagnosable at enrollment would be delayed 1year instead. Cost-effectiveness analyses are needed. Published by Elsevier Inc.
The Astrobiology in Secondary Classrooms (ASC) curriculum: focusing upon diverse students and teachers.

PubMed

Arino de la Rubia, Leigh S

2012-09-01

The Minority Institution Astrobiology Collaborative (MIAC) began working with the NASA Goddard Center for Astrobiology in 2003 to develop curriculum materials for high school chemistry and Earth science classes based on astrobiology concepts. The Astrobiology in Secondary Classrooms (ASC) modules emphasize interdisciplinary connections in astronomy, biology, chemistry, geoscience, physics, mathematics, and ethics through hands-on activities that address national educational standards. Field-testing of the Astrobiology in Secondary Classrooms materials occurred over three years in eight U.S. locations, each with populations that are underrepresented in the career fields of science, technology, engineering, and mathematics. Analysis of the educational research upon the high school students participating in the ASC project showed statistically significant increases in students' perceived knowledge and science reasoning. The curriculum is in its final stages, preparing for review to become a NASA educational product.
Inhibition of caspase-1 or gasdermin-D enable caspase-8 activation in the Naip5/NLRC4/ASC inflammasome

PubMed Central

Pereira, Marcelo S. F.; Manin, Graziele Z.; Cunha, Larissa D.

2017-01-01

Legionella pneumophila is a Gram-negative, flagellated bacterium that survives in phagocytes and causes Legionnaires’ disease. Upon infection of mammalian macrophages, cytosolic flagellin triggers the activation of Naip/NLRC4 inflammasome, which culminates in pyroptosis and restriction of bacterial replication. Although NLRC4 and caspase-1 participate in the same inflammasome, Nlrc4-/- mice and their macrophages are more permissive to L. pneumophila replication compared with Casp1/11-/-. This feature supports the existence of a pathway that is NLRC4-dependent and caspase-1/11-independent. Here, we demonstrate that caspase-8 is recruited to the Naip5/NLRC4/ASC inflammasome in response to flagellin-positive bacteria. Accordingly, caspase-8 is activated in Casp1/11-/- macrophages in a process dependent on flagellin, Naip5, NLRC4 and ASC. Silencing caspase-8 in Casp1/11-/- cells culminated in macrophages that were as susceptible as Nlrc4-/- for the restriction of L. pneumophila replication. Accordingly, macrophages and mice deficient in Asc/Casp1/11-/- were more susceptible than Casp1/11-/- and as susceptible as Nlrc4-/- for the restriction of infection. Mechanistically, we found that caspase-8 activation triggers gasdermin-D-independent pore formation and cell death. Interestingly, caspase-8 is recruited to the Naip5/NLRC4/ASC inflammasome in wild-type macrophages, but it is only activated when caspase-1 or gasdermin-D is inhibited. Our data suggest that caspase-8 activation in the Naip5/NLRC4/ASC inflammasome enable induction of cell death when caspase-1 or gasdermin-D is suppressed. PMID:28771586
Inhibition of caspase-1 or gasdermin-D enable caspase-8 activation in the Naip5/NLRC4/ASC inflammasome.

PubMed

Mascarenhas, Danielle P A; Cerqueira, Daiane M; Pereira, Marcelo S F; Castanheira, Fernanda V S; Fernandes, Talita D; Manin, Graziele Z; Cunha, Larissa D; Zamboni, Dario S

2017-08-01

Legionella pneumophila is a Gram-negative, flagellated bacterium that survives in phagocytes and causes Legionnaires' disease. Upon infection of mammalian macrophages, cytosolic flagellin triggers the activation of Naip/NLRC4 inflammasome, which culminates in pyroptosis and restriction of bacterial replication. Although NLRC4 and caspase-1 participate in the same inflammasome, Nlrc4-/- mice and their macrophages are more permissive to L. pneumophila replication compared with Casp1/11-/-. This feature supports the existence of a pathway that is NLRC4-dependent and caspase-1/11-independent. Here, we demonstrate that caspase-8 is recruited to the Naip5/NLRC4/ASC inflammasome in response to flagellin-positive bacteria. Accordingly, caspase-8 is activated in Casp1/11-/- macrophages in a process dependent on flagellin, Naip5, NLRC4 and ASC. Silencing caspase-8 in Casp1/11-/- cells culminated in macrophages that were as susceptible as Nlrc4-/- for the restriction of L. pneumophila replication. Accordingly, macrophages and mice deficient in Asc/Casp1/11-/- were more susceptible than Casp1/11-/- and as susceptible as Nlrc4-/- for the restriction of infection. Mechanistically, we found that caspase-8 activation triggers gasdermin-D-independent pore formation and cell death. Interestingly, caspase-8 is recruited to the Naip5/NLRC4/ASC inflammasome in wild-type macrophages, but it is only activated when caspase-1 or gasdermin-D is inhibited. Our data suggest that caspase-8 activation in the Naip5/NLRC4/ASC inflammasome enable induction of cell death when caspase-1 or gasdermin-D is suppressed.
76 FR 33994 - Alternative Simplified Credit Under Section 41(c)(5)

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-10

... Alternative Simplified Credit Under Section 41(c)(5) AGENCY: Internal Revenue Service (IRS), Treasury. ACTION... regulations relating to the election and calculation of the alternative simplified credit under section 41(c... 41(c)(5). The ASC was added by the Tax Relief and Health Care Act of 2006 (Public Law 109-432, 120...
Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation.

PubMed

Kwak, Su-Bin; Koppula, Sushruta; In, Eun-Jung; Sun, Xiao; Kim, Young-Kyu; Kim, Myong-Ki; Lee, Kwang-Ho; Kang, Tae-Bong

2018-01-01

Artemisia princeps var. orientalis (Asteraceae, A. princeps ) is a well-known traditional medicinal herb used for treating various inflammatory disorders in Korea, Japan, China, and other Asian countries. In the present study, we investigated the effects of A. princeps extract (APO) on interleukin- (IL-) 1 β regulation and inflammasome activation in bone marrow-derived macrophages (BMDMs) and monosodium urate- (MSU-) induced peritonitis mouse model in vivo . The APO treatment to BMDMs primed with lipopolysaccharide (LPS) attenuated the NLRP3 and AIM2 inflammasome activation induced by danger signals, such as ATP, nigericin, silica crystals, and poly (dA:dT), respectively. Mechanistic study revealed that APO suppressed the ASC oligomerization and speck formation, which are required for inflammasome activation. APO treatment also reduced the ASC phosphorylation induced by the combination of LPS and a tyrosine phosphatase inhibitor. In vivo evaluation revealed that intraperitoneal administration of APO reduced IL-1 β levels, significantly ( p < 0.05) and dose dependently, in the MSU-induced peritonitis mouse model. In conclusion, our study is the first to report that the extract of A. princeps inhibits inflammasome activation through the modulation of ASC phosphorylation. Therefore, APO might be developed as therapeutic potential in the treatment of inflammasome-mediated inflammatory disorders, such as gouty arthritis.
Artemisia Extract Suppresses NLRP3 and AIM2 Inflammasome Activation by Inhibition of ASC Phosphorylation

PubMed Central

Kwak, Su-Bin; Koppula, Sushruta; In, Eun-Jung; Sun, Xiao; Kim, Young-Kyu

2018-01-01

Artemisia princeps var. orientalis (Asteraceae, A. princeps) is a well-known traditional medicinal herb used for treating various inflammatory disorders in Korea, Japan, China, and other Asian countries. In the present study, we investigated the effects of A. princeps extract (APO) on interleukin- (IL-) 1β regulation and inflammasome activation in bone marrow-derived macrophages (BMDMs) and monosodium urate- (MSU-) induced peritonitis mouse model in vivo. The APO treatment to BMDMs primed with lipopolysaccharide (LPS) attenuated the NLRP3 and AIM2 inflammasome activation induced by danger signals, such as ATP, nigericin, silica crystals, and poly (dA:dT), respectively. Mechanistic study revealed that APO suppressed the ASC oligomerization and speck formation, which are required for inflammasome activation. APO treatment also reduced the ASC phosphorylation induced by the combination of LPS and a tyrosine phosphatase inhibitor. In vivo evaluation revealed that intraperitoneal administration of APO reduced IL-1β levels, significantly (p < 0.05) and dose dependently, in the MSU-induced peritonitis mouse model. In conclusion, our study is the first to report that the extract of A. princeps inhibits inflammasome activation through the modulation of ASC phosphorylation. Therefore, APO might be developed as therapeutic potential in the treatment of inflammasome-mediated inflammatory disorders, such as gouty arthritis. PMID:29686531
The influence of food supply on the response of Olympia oyster larvae to ocean acidification

NASA Astrophysics Data System (ADS)

Hettinger, A.; Sanford, E.; Hill, T. M.; Hosfelt, J. D.; Russell, A. D.; Gaylord, B.

2013-10-01

Increases in atmospheric carbon dioxide drive accompanying changes in the marine carbonate system as carbon dioxide (CO2) enters seawater and alters ocean pH (termed "ocean acidification"). However, such changes do not occur in isolation, and other environmental factors have the potential to modulate the consequences of altered ocean chemistry. Given that physiological mechanisms used by organisms to confront acidification can be energetically costly, we explored the potential for food supply to influence the response of Olympia oyster (Ostrea lurida) larvae to ocean acidification. In laboratory experiments, we reared oyster larvae under a factorial combination of pCO2 and food level. Elevated pCO2 had negative effects on larval growth, total dry weight, and metamorphic success, but high food availability partially offset these influences. The combination of elevated pCO2 and low food availability led to the greatest reduction in larval performance. However, the effects of food and pCO2 interacted additively rather than synergistically, indicating that they operated independently. Despite the potential for abundant resources to counteract the consequences of ocean acidification, impacts were never completely negated, suggesting that even under conditions of enhanced primary production and elevated food availability, impacts of ocean acidification may still accrue in some consumers.

78 FR 54214 - Endangered and Threatened Wildlife and Plants; Removing Five Subspecies of Mazama Pocket Gopher...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-03

... section 3 of the Act, an endangered species is any species which is in danger of extinction throughout all... populations, do not occur elsewhere. Our Response: The presumption of extinction for the Tacoma pocket gopher... contrast to our presumption of extinction for the Tacoma pocket gopher, which is based on evidence from...
Activation of phagocytic activity in astrocytes by reduced expression of the inflammasome component ASC and its implication in a mouse model of Alzheimer disease.

PubMed

Couturier, Julien; Stancu, Ilie-Cosmin; Schakman, Olivier; Pierrot, Nathalie; Huaux, François; Kienlen-Campard, Pascal; Dewachter, Ilse; Octave, Jean-Noël

2016-01-27

The proinflammatory cytokine interleukin-1β (IL-1β) is overexpressed in Alzheimer disease (AD) as a key regulator of neuroinflammation. Amyloid-β (Aβ) peptide triggers activation of inflammasomes, protein complexes responsible for IL-1β maturation in microglial cells. Downregulation of NALP3 (NACHT, LRR, and PYD domains-containing protein 3) inflammasome has been shown to decrease amyloid load and rescue cognitive deficits in a mouse model of AD. Whereas activation of inflammasome in microglial cells has been described in AD, no data are currently available concerning activation of inflammasome in astrocytes, although they are involved in inflammatory response and phagocytosis. Here, by targeting the inflammasome adaptor protein ASC (apoptosis-associated speck-like protein containing a CARD domain), we investigated the influence of activation of the inflammasome on the phagocytic activity of astrocytes. We used an ASC knockout mouse model, as ASC is a central protein in the inflammasome, acting as an adaptor and stabilizer of the complex and thus critical for its activation. Lipopolysaccharide (LPS)-primed primary cultures of astrocytes from newborn mice were utilized to evaluate Aβ-induced inflammasome activation by measuring IL-1β release by ECLIA (electro-chemiluminescence immunoassay). Phagocytosis efficiency was measured by incorporation of bioparticles, and the release of the chemokine CCL3 (C-C motif ligand 3) was measured by ECLIA. ASC mice were crossbred with 5xFAD (familial Alzheimer disease) mice and tested for spatial reference memory using the Morris water maze (MWM) at 7-8 months of age. Amyloid load and CCL3 were quantified by thioflavine S staining and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. Cultured astrocytes primed with LPS and treated with Aβ showed an ASC-dependent production of IL-1β resulting from inflammasome activation mediated by Aβ phagocytosis and cathepsin B enzymatic activity. ASC
Active shortening of the Cascadia forearc and implications for seismic hazards of the Puget Lowland

USGS Publications Warehouse

Johnson, S.Y.; Blakely, R.J.; Stephenson, W.J.; Dadisman, S.V.; Fisher, M.A.

2004-01-01

Margin-parallel shortening of the Cascadia forearc is a consequence of oblique subduction of the Juan de Fuca plate beneath North America. Strike-slip, thrust, and oblique crustal faults beneath the densely populated Puget Lowland accommodate much of this north-south compression, resulting in large crustal earthquakes. To better understand this forearc deformation and improve earthquake hazard, assessment, we here use seismic reflection surveys, coastal exposures of Pleistocene strata, potential-field data, and airborne laser swath mapping to document and interpret a significant structural boundary near the City of Tacoma. This boundary is a complex structural zone characterized by two distinct segments. The northwest trending, eastern segment, extending from Tacoma to Carr Inlet, is formed by the broad (??? 11.5 km), southwest dipping (??? 11??-2??) Rosedale monocline. This monocline raises Crescent Formation basement about 2.5 km, resulting in a moderate gravity gradient. We interpret the Rosedale monocline as a fault-bend fold, forming above a deep thrust fault. Within the Rosedale monocline, inferred Quaternary strata thin northward and form a growth triangle that is 4.1 to 6.6 km wide at its base, suggesting ??? 2-3 mm/yr of slip on the underlying thrust. The western section of the >40-km-long, north dipping Tacoma fault, extending from Hood Canal to Carr Inlet, forms the western segment of the Tacoma basin margin. Structural relief on this portion of the basin margin may be several kilometers, resulting in steep gravity and aeromagnetic anomalies. Quaternary structural relief along the Tacoma fault is as much as 350-400 m, indicating a minimum slip rate of about 0.2 mm/yr. The inferred eastern section of the Tacoma fault (east of Carr Inlet) crosses the southern part of the Seattle uplift, has variable geometry along strike, and diminished structural relief. The Tacoma fault is regarded as a north dipping backthrust to the Seattle fault, so that slip on a
Commencement Bay Nearshore/Tideflats Superfund Site, Tacoma, Washington Remedial Investigations. Evaluation of Alternative Dredging Methods and Equipment, Disposal Methods and Sites, and Site Control and Treatment Practices for Contaminated Sediments.

DTIC Science & Technology

1985-06-01

material may have on ground water and drain- age systems and the possible burial of hazardous materials already existing on the site. The Port of Tacoma is...good resistance to inorganic chemicals; high gas permeability Asphalt- Core layer Of blown asphalt M Flexible enough to conform to Ages rapidly in hot...Tendst to harden on aging ; low polyethylene reacting polyethylene with acids, and alkalis tensile strength; tenoericy to chlorine and sulfur dioxide
Draft Genome Sequence of Heavy Metal-Resistant Cupriavidus alkaliphilus ASC-732 T, Isolated from Agave Rhizosphere in the Northeast of Mexico

DOE PAGES

Rojas-Rojas, Fernando Uriel; Huntemann, Marcel; Clum, Alicia; ...

2016-09-22

Cupriavidus alkaliphilus ASC-732 T was isolated from the rhizosphere of agave plant growing in alkaline soils in San Carlos, Tamaulipas, Mexico. The species is able to grow in the presence of arsenic, zinc, and copper. The genome sequence of strain ASC-732 T is 6,125,055 bp with 5,586 genes and an average G+C content of 67.81%.
Draft Genome Sequence of Heavy Metal-Resistant Cupriavidus alkaliphilus ASC-732 T, Isolated from Agave Rhizosphere in the Northeast of Mexico

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rojas-Rojas, Fernando Uriel; Huntemann, Marcel; Clum, Alicia

Cupriavidus alkaliphilus ASC-732 T was isolated from the rhizosphere of agave plant growing in alkaline soils in San Carlos, Tamaulipas, Mexico. The species is able to grow in the presence of arsenic, zinc, and copper. The genome sequence of strain ASC-732 T is 6,125,055 bp with 5,586 genes and an average G+C content of 67.81%.
Iterative Focused Screening with Biological Fingerprints Identifies Selective Asc-1 Inhibitors Distinct from Traditional High Throughput Screening.

PubMed

Kutchukian, Peter S; Warren, Lee; Magliaro, Brian C; Amoss, Adam; Cassaday, Jason A; O'Donnell, Gregory; Squadroni, Brian; Zuck, Paul; Pascarella, Danette; Culberson, J Chris; Cooke, Andrew J; Hurzy, Danielle; Schlegel, Kelly-Ann Sondra; Thomson, Fiona; Johnson, Eric N; Uebele, Victor N; Hermes, Jeffrey D; Parmentier-Batteur, Sophie; Finley, Michael

2017-02-17

N-methyl-d-aspartate receptors (NMDARs) mediate glutamatergic signaling that is critical to cognitive processes in the central nervous system, and NMDAR hypofunction is thought to contribute to cognitive impairment observed in both schizophrenia and Alzheimer's disease. One approach to enhance the function of NMDAR is to increase the concentration of an NMDAR coagonist, such as glycine or d-serine, in the synaptic cleft. Inhibition of alanine-serine-cysteine transporter-1 (Asc-1), the primary transporter of d-serine, is attractive because the transporter is localized to neurons in brain regions critical to cognitive function, including the hippocampus and cortical layers III and IV, and is colocalized with d-serine and NMDARs. To identify novel Asc-1 inhibitors, two different screening approaches were performed with whole-cell amino acid uptake in heterologous cells stably expressing human Asc-1: (1) a high-throughput screen (HTS) of 3 M compounds measuring 35 S l-cysteine uptake into cells attached to scintillation proximity assay beads in a 1536 well format and (2) an iterative focused screen (IFS) of a 45 000 compound diversity set using a 3 H d-serine uptake assay with a liquid scintillation plate reader in a 384 well format. Critically important for both screening approaches was the implementation of counter screens to remove nonspecific inhibitors of radioactive amino acid uptake. Furthermore, a 15 000 compound expansion step incorporating both on- and off-target data into chemical and biological fingerprint-based models for selection of additional hits enabled the identification of novel Asc-1-selective chemical matter from the IFS that was not identified in the full-collection HTS.
HPV-DNA testing for patients with ASC-US helps identify the women who have a high risk for precancerous cervical lesions.

PubMed

Moarcăs, M; Georgescu, I C; Moarcăs, R; Badea, M; Cîrstoiu, M

2014-01-01

The cytological interpretation of ASC-US represents a category of morphologic uncertainty. For patients with this result, other tests are necessary in order to determine the risk for cervical lesions. 198 patients with ASC-US cytology have been analyzed between 2008 and 2013. All the patients included in the study have subsequently had a high oncogenic HPV testing and colposcopy risk. 103 (52%) patients tested positive for high risk HPV and 21 (10%) had associated colposcopy changes and precancerous and cancerous lesions identified through biopsy. 95 (48%) patients tested negative for HPV and none of these women had lesions at colposcopy. High oncogenic risk HPV testing was proven useful in identifying the patients with ASC-US cytology who are at high risk for cervical lesions (100% sensibility). In this study, the HPV testing had a negative predictive value of 100%, which uselessly renders a further colposcopy evaluation. HPV testing for women with ASC-US is not specific in identifying women with cervical lesions (Specificity 53%) and this results from a high prevalence of limited HPV infections in an age group which is less than 30 years old. High risk HPV testing for women with ASC-US cervical cytology is useful in determining the risk for precancerous and cancerous cervical lesions. A positive result is associated with a high risk for cervical lesions (20%) and for these patients colposcopy is necessary. For women with a negative result, the risk for cervical lesions is practically null so colposcopy is not required.
Environmental impact of non-certified versus certified (ASC) intensive Pangasius aquaculture in Vietnam, a comparison based on a statistically supported LCA.

PubMed

Nhu, Trang T; Schaubroeck, Thomas; Henriksson, Patrik J G; Bosma, Roel; Sorgeloos, Patrick; Dewulf, Jo

2016-12-01

Pangasius production in Vietnam is widely known as a success story in aquaculture, the fastest growing global food system because of its tremendous expansion by volume, value and the number of international markets to which Pangasius has been exported in recent years. While certification schemes are becoming significant features of international fish trade and marketing, an increasing number of Pangasius producers have followed at least one of the certification schemes recognised by international markets to incorporate environmental and social sustainability practices in aquaculture, typically the Pangasius Aquaculture Dialogue (PAD) scheme certified by the Aquaculture Stewardship Council (ASC). An assessment of the environmental benefit of applying certification schemes on Pangasius production, however, is still needed. This article compared the environmental impact of ASC-certified versus non-ASC certified intensive Pangasius aquaculture, using a statistically supported LCA. We focused on both resource-related (water, land and total resources) and emissions-related (global warming, acidification, freshwater and marine eutrophication) categories. The ASC certification scheme was shown to be a good approach for determining adequate environmental sustainability, especially concerning emissions-related categories, in Pangasius production. However, the non-ASC certified farms, due to the large spread, the impact (e.g., water resources and freshwater eutrophication) was possibly lower for a certain farm. However, this result was not generally prominent. Further improvements in intensive Pangasius production to inspire certification schemes are proposed, e.g., making the implementation of certification schemes more affordable, well-oriented and facilitated; reducing consumed feed amounts and of the incorporated share in fishmeal, especially domestic fishmeal, etc. However, their implementation should be vetted with key stakeholders to assess their feasibility. Copyright
The effect of magnetic stimulation on the osteogenic and chondrogenic differentiation of human stem cells derived from the adipose tissue (hASCs)

NASA Astrophysics Data System (ADS)

Lima, João; Gonçalves, Ana I.; Rodrigues, Márcia T.; Reis, Rui L.; Gomes, Manuela E.

2015-11-01

The use of magnetic nanoparticles (MNPs) towards the musculoskeletal tissues has been the focus of many studies, regarding MNPs ability to promote and direct cellular stimulation and orient tissue responses. This is thought to be mainly achieved by mechano-responsive pathways, which can induce changes in cell behavior, including the processes of proliferation and differentiation, in response to external mechanical stimuli. Thus, the application of MNP-based strategies in tissue engineering may hold potential to propose novel solutions for cell therapy on bone and cartilage strategies to accomplish tissue regeneration. The present work aims at studying the influence of MNPs on the osteogenic and chondrogenic differentiation of human adipose derived stem cells (hASCs). MNPs were incorporated in hASCs and cultured in medium supplemented for osteogenic and chondrogenic differentiation. Cultures were maintained up to 28 days with/without an external magnetic stimulus provided by a magnetic bioreactor, to determine if the MNPs alone could affect the osteogenic or chondrogenic phenotype of the hASCs. Results indicate that the incorporation of MNPs does not negatively affect the viability nor the proliferation of hASCs. Furthermore, Alizarin Red staining evidences an enhancement in extracellular (ECM) mineralization under the influence of an external magnetic field. Although not as evident as for osteogenic differentiation, Toluidine blue and Safranin-O stainings also suggest the presence of a cartilage-like ECM with glycosaminoglycans and proteoglycans under the magnetic stimulus provided. Thus, MNPs incorporated in hASCs under the influence of an external magnetic field have the potential to induce differentiation towards the osteogenic and chondrogenic lineages.
Field and Laboratory Data From an Earthquake History Study of Scarps in the Hanging Wall of the Tacoma Fault, Mason and Pierce Counties, Washington

USGS Publications Warehouse

Nelson, Alan R.; Personius, Stephen F.; Sherrod, Brian L.; Buck, Jason; Bradley, Lee-Ann; Henley, Gary; Liberty, Lee M.; Kelsey, Harvey M.; Witter, Robert C.; Koehler, R.D.; Schermer, Elizabeth R.; Nemser, Eliza S.; Cladouhos, Trenton T.

2008-01-01

As part of the effort to assess seismic hazard in the Puget Sound region, we map fault scarps on Airborne Laser Swath Mapping (ALSM, an application of LiDAR) imagery (with 2.5-m elevation contours on 1:4,000-scale maps) and show field and laboratory data from backhoe trenches across the scarps that are being used to develop a latest Pleistocene and Holocene history of large earthquakes on the Tacoma fault. We supplement previous Tacoma fault paleoseismic studies with data from five trenches on the hanging wall of the fault. In a new trench across the Catfish Lake scarp, broad folding of more tightly folded glacial sediment does not predate 4.3 ka because detrital charcoal of this age was found in stream-channel sand in the trench beneath the crest of the scarp. A post-4.3-ka age for scarp folding is consistent with previously identified uplift across the fault during AD 770-1160. In the trench across the younger of the two Stansberry Lake scarps, six maximum 14C ages on detrital charcoal in pre-faulting B and C soil horizons and three minimum ages on a tree root in post-faulting colluvium, limit a single oblique-slip (right-lateral) surface faulting event to AD 410-990. Stratigraphy and sedimentary structures in the trench across the older scarp at the same site show eroded glacial sediments, probably cut by a meltwater channel, with no evidence of post-glacial deformation. At the northeast end of the Sunset Beach scarps, charcoal ages in two trenches across graben-forming scarps give a close maximum age of 1.3 ka for graben formation. The ages that best limit the time of faulting and folding in each of the trenches are consistent with the time of the large regional earthquake in southern Puget Sound about AD 900-930.
Optimizing the ASC WAN: evaluating network performance tools for comparing transport protocols.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lydick, Christopher L.

2007-07-01

The Advanced Simulation & Computing Wide Area Network (ASC WAN), which is a high delay-bandwidth network connection between US Department of Energy National Laboratories, is constantly being examined and evaluated for efficiency. One of the current transport-layer protocols which is used, TCP, was developed for traffic demands which are different from that on the ASC WAN. The Stream Control Transport Protocol (SCTP), on the other hand, has shown characteristics which make it more appealing to networks such as these. Most important, before considering a replacement for TCP on any network, a testing tool that performs well against certain criteria needsmore » to be found. In order to try to find such a tool, two popular networking tools (Netperf v.2.4.3 & v.2.4.6 (OpenSS7 STREAMS), and Iperf v.2.0.6) were tested. These tools implement both TCP and SCTP and were evaluated using four metrics: (1) How effectively can the tool reach a throughput near the bandwidth? (2) How much of the CPU does the tool utilize during operation? (3) Is the tool freely and widely available? And, (4) Is the tool actively developed? Following the analysis of those tools, this paper goes further into explaining some recommendations and ideas for future work.« less
Development of agent-based on-line adaptive signal control (ASC) framework using connected vehicle (CV) technology.

DOT National Transportation Integrated Search

2016-04-01

In this study, we developed an adaptive signal control (ASC) framework for connected vehicles (CVs) using agent-based modeling technique. : The proposed framework consists of two types of agents: 1) vehicle agents (VAs); and 2) signal controller agen...
Program to convert SUDS2ASC files to a single binary SEGY file

USGS Publications Warehouse

Goldman, Mark

2000-01-01

This program, SUDS2SEGY, converts and combines ASCII files created using SUDS2ASC Version 2.60, to a single SEGY file. SUDS2ASC has been used previously to create an ASCII file of three-component seismic data for an individual recording station. However, many seismic processing packages have difficulty reading in ASCII data. In addition, it may be cumbersome to process a separate file for each recording station, particularly if traces from different recording stations contain a different number of data samples and/or a different start time. This new program - SUDS2SEGY - combines these recording station files into a single SEGY file. In addition, SUDS2SEGY normalizes the trace times so that each trace starts at a given time and consists of a fixed number of samples. This normalization allows seismic data from many different stations to be read in as a single "data gather". SUDS2SEGY also produces a report summarizing the offset and maximum absolute amplitude for each component in a station file. These data are output separately to an ASCII file and can be subsequently input to a plotting package.
Sulfate-rich eolian and wet interdune deposits, erebus crater, meridiani Planum, Mars

USGS Publications Warehouse

Metz, J.M.; Grotzinger, J.P.; Rubin, D.M.; Lewis, K.W.; Squyres, S. W.; Bell, J.F.

2009-01-01

This study investigates three bedrock exposures at Erebus crater, an ?? 300 m diameter crater approximately 4 km south of Endurance crater on Mars. These outcrops, called Olympia, Payson, and Yavapai, provide additional evidence in support of the dune-interdune model proposed for the formation of the deposits at the Opportunity landing site in Meridiani Planum. There is evidence for greater involvement of liquid water in the Olympia outcrop exposures than was observed in Eagle or Endurance craters. The Olympia outcrop likely formed in a wet interdune and sand sheet environment. The facies observed within the Payson outcrop, which is likely stratigraphically above the Olympia outcrop, indicate that it was deposited in a damp-wet interdune, sand sheet, and eolian dune environment. The Yavapai outcrop, which likely stratigraphically overlies the Payson outcrop, indicates that it was deposited in primarily a sand sheet environment and also potentially in an eolian dune environment. These three outcrop exposures may indicate an overall drying-upward trend spanning the stratigraphic section from its base at the Olympia outcrop to its top at the Yavapai outcrop. This contrasts with the wetting-upward trend seen in Endurance and Eagle craters. Thus, the series of outcrops seen at Meridiani by Opportunity may constitute a full climatic cycle, evolving from dry to wet to dry conditions. ?? 2009, SEPM (Society for Sedimentary Geology).
ASC FY17 Implementation Plan, Rev. 1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hamilton, P. G.

The Stockpile Stewardship Program (SSP) is an integrated technical program for maintaining the safety, surety, and reliability of the U.S. nuclear stockpile. The SSP uses nuclear test data, computational modeling and simulation, and experimental facilities to advance understanding of nuclear weapons. It includes stockpile surveillance, experimental research, development and engineering programs, and an appropriately scaled production capability to support stockpile requirements. This integrated national program requires the continued use of experimental facilities and programs, and the computational capabilities to support these programs. The Advanced Simulation and Computing Program (ASC) is a cornerstone of the SSP, providing simulation capabilities and computationalmore » resources that support annual stockpile assessment and certification, study advanced nuclear weapons design and manufacturing processes, analyze accident scenarios and weapons aging, and provide the tools to enable stockpile Life Extension Programs (LEPs) and the resolution of Significant Finding Investigations (SFIs). This requires a balance of resources, including technical staff, hardware, simulation software, and computer science solutions.« less
Report of experiments and evidence for ASC L2 milestone 4467 : demonstration of a legacy application's path to exascale.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Curry, Matthew L.; Ferreira, Kurt Brian; Pedretti, Kevin Thomas Tauke

2012-03-01

This report documents thirteen of Sandia's contributions to the Computational Systems and Software Environment (CSSE) within the Advanced Simulation and Computing (ASC) program between fiscal years 2009 and 2012. It describes their impact on ASC applications. Most contributions are implemented in lower software levels allowing for application improvement without source code changes. Improvements are identified in such areas as reduced run time, characterizing power usage, and Input/Output (I/O). Other experiments are more forward looking, demonstrating potential bottlenecks using mini-application versions of the legacy codes and simulating their network activity on Exascale-class hardware. The purpose of this report is to provemore » that the team has completed milestone 4467-Demonstration of a Legacy Application's Path to Exascale. Cielo is expected to be the last capability system on which existing ASC codes can run without significant modifications. This assertion will be tested to determine where the breaking point is for an existing highly scalable application. The goal is to stretch the performance boundaries of the application by applying recent CSSE RD in areas such as resilience, power, I/O, visualization services, SMARTMAP, lightweight LWKs, virtualization, simulation, and feedback loops. Dedicated system time reservations and/or CCC allocations will be used to quantify the impact of system-level changes to extend the life and performance of the ASC code base. Finally, a simulation of anticipated exascale-class hardware will be performed using SST to supplement the calculations. Determine where the breaking point is for an existing highly scalable application: Chapter 15 presented the CSSE work that sought to identify the breaking point in two ASC legacy applications-Charon and CTH. Their mini-app versions were also employed to complete the task. There is no single breaking point as more than one issue was found with the two codes. The results were that
Fluorescent Immortalized Human Adipose Derived Stromal Cells (hASCs-TS/GFP+) for Studying Cell Drug Delivery Mediated by Microvesicles.

PubMed

Cocce, Valentina; Balducci, Luigi; Falchetti, Maria L; Pascucci, Luisa; Ciusani, Emilio; Brini, Anna T; Sisto, Francesca; Piovani, Giovanna; Alessandri, Giulio; Parati, Eugenio; Cabeza, Laura; Pessina, Augusto

2017-11-24

A new tool for the drug delivery is based on the use of Mesenchymal Stromal Cells (MSCs) loaded in vitro with anti-cancer drugs. Unfortunately, the restricted lifespan of MSCs represents a significant limitation to produce them in high amounts and for long time studies. Immortalized MSCs from adipose tissue (hASCs) have been generated as good source of cells with stable features. These cells could improve the development of standardized procedures for both in vitro and preclinical studies. Furthermore they facilitate procedures for preparing large amounts of secretome containing microvesicles (MVs). We used human adipose tissue derived MSCs immortalized with hTERT+SV40 (TS) genes and transfected with GFP (hASCs-TS/GFP+). This line was investigated for its ability to uptake and release anticancer drugs. Microvesicles associated to paclitaxel (MVs/PTX) were isolated, quantified, and tested on pancreatic cancer cells. The line hASCs-TS/GFP+ maintained the main mesenchymal characters and was able to uptake and release, in active form, both paclitaxel and gemcitabine. From paclitaxel loaded hASCs-TS/GFP+ cells were isolated microvesicles in sufficient amount to inhibit "in vitro" the proliferation of pancreatic tumor cells. Our study suggests that human immortalized MSCs could be used for a large scale production of cells for mediated drug delivery. Moreover, the secretion of drug-associated MVs could represent a new way for producing new drug formulation by "biogenesis". In the context of the "advanced cell therapy procedure", the MVs/PTX production would use less resource and time and it could possibly contribute to simplification of GMP procedures. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.
Prediction of cervical intraepithelial neoplasia grade 2+ (CIN2+) using HPV DNA testing after a diagnosis of atypical squamous cell of undetermined significance (ASC-US) in Catalonia, Spain.

PubMed

Ibáñez, Raquel; Moreno-Crespi, Judit; Sardà, Montserrat; Autonell, Josefina; Fibla, Montserrat; Gutiérrez, Cristina; Lloveras, Belen; Alejo, María; Català, Isabel; Alameda, Francesc; Casas, Miquel; Bosch, F Xavier; de Sanjosé, Silvia

2012-01-26

A protocol for cervical cancer screening among sexually active women 25 to 65 years of age was introduced in 2006 in Catalonia, Spain to increase coverage and to recommend a 3-year-interval between screening cytology. In addition, Human Papillomavirus (HPV) was offered as a triage test for women with a diagnosis of atypical squamous cells of undetermined significance (ASC-US). HPV testing was recommended within 3 months of ASC-US diagnosis. According to protocol, HPV negative women were referred to regular screening including a cytological exam every 3 years while HPV positive women were referred to colposcopy and closer follow-up. We evaluated the implementation of the protocol and the prediction of HPV testing as a triage tool for cervical intraepithelial lesions grade two or worse (CIN2+) in women with a cytological diagnosis of ASC-US. During 2007-08 a total of 611 women from five reference laboratories in Catalonia with a novel diagnosis of ASC-US were referred for high risk HPV (hrHPV) triage using high risk Hybrid Capture version 2. Using routine record linkage data, women were followed for 3 years to evaluate hrHPV testing efficacy for predicting CIN2+ cases. Logistic regression analysis was used to estimate the odds ratio for CIN2 +. Among the 611 women diagnosed with ASC-US, 493 (80.7%) had at least one follow-up visit during the study period. hrHPV was detected in 48.3% of the women at study entry (mean age 35.2 years). hrHPV positivity decreased with increasing age from 72.6% among women younger than 25 years to 31.6% in women older than 54 years (p < 0.01). At the end of the 3 years follow-up period, 37 women with a diagnosis of CIN2+ (18 CIN2, 16 CIN3, 2 cancers, and 1 with high squamous intraepithelial lesions--HSIL) were identified and all but one had a hrHPV positive test at study entry. Sensitivity to detect CIN2+ of hrHPV was 97.2% (95%confidence interval (CI) = 85.5-99.9) and specificity was 68.3% (95%CI = 63.1-73.2). The odds ratio for CIN2
Inflammasome components ASC and AIM2 modulate the acute phase of biomaterial implant-induced foreign body responses.

PubMed

Christo, Susan N; Diener, Kerrilyn R; Manavis, Jim; Grimbaldeston, Michele A; Bachhuka, Akash; Vasilev, Krasimir; Hayball, John D

2016-02-10

Detailing the inflammatory mechanisms of biomaterial-implant induced foreign body responses (FBR) has implications for revealing targetable pathways that may reduce leukocyte activation and fibrotic encapsulation of the implant. We have adapted a model of poly(methylmethacrylate) (PMMA) bead injection to perform an assessment of the mechanistic role of the ASC-dependent inflammasome in this process. We first demonstrate that ASC(-/-) mice subjected to PMMA bead injections had reduced cell infiltration and altered collagen deposition, suggesting a role for the inflammasome in the FBR. We next investigated the NLRP3 and AIM2 sensors because of their known contributions in recognising damaged and apoptotic cells. We found that NLRP3 was dispensable for the fibrotic encapsulation; however AIM2 expression influenced leukocyte infiltration and controlled collagen deposition, suggesting a previously unexplored link between AIM2 and biomaterial-induced FBR.

Enlarged squamous cell nuclei in cervical cytologic specimens from perimenopausal women ("PM Cells") : a cause of ASC overdiagnosis.

PubMed

Cibas, Edmund S; Browne, Tara-Jane; Bassichis, Michelle H Mantel; Lee, Kenneth R

2005-07-01

We studied the appropriateness of interpreting squamous cells with enlarged, smooth, bland nuclei in perimenopausal women ("PM cells") as atypical squamous cells (ASCs). Papanicolaou smears (Paps) from 100 women (40-55 years old) with a cytologic interpretation of ASC of undetermined significance (ASCUS) and human papillomavirus (HPV) testing or a biopsy within 6 months were reviewed by 2 observers without knowledge of the biopsy diagnosis or HPV results. Cases in which both reviewers agreed that the Paps were diagnosed more properly as "negative for intraepithelial lesion or malignancy" were compared with cases of "true ASCUS," using histologic squamous intraepithelial lesion and/or a positive high-risk HPV test as a positive outcome (abnormal follow-up). Of 100 cases, 28 were reclassified as benign by both observers. In 15 of these, the original ASCUS interpretation was based on cells with bland nuclear enlargement (2-3 times the area of intermediate cell nuclei), smooth nuclear membranes, and fine chromatin. Abnormal follow-up was identified in 1 (7%) of 15 benign cases but in 30 (42%) of 72 true ASCUS cases (P = .023). PM cells are a significant cause of ASC overdiagnosis in women 40 to 55 years old. Cervical Paps with cells no more atypical than these can be interpreted safely as negative for intraepithelial lesion or malignancy.
Clinical significance of HPV DNA cotesting in Korean women with ASCUS or ASC-H.

PubMed

Lee, Sanghoon; Kim, Jae Won; Hong, Jin Hwa; Song, Jae Yun; Lee, Jae Kwan; Kim, In Sun; Lee, Nak Woo

2014-12-01

The purpose of this study was to evaluate the clinical significance of Human papillomavirus (HPV) DNA cotesting in Korean women with abnormal Papanicolaou (Pap) smear results based on colposcopic pathology. A total of 1012 women underwent liquid-based Pap smears and hybrid capture II HPV DNA tests followed by colposcopy at the Korea University Hospital from January 2007 to May 2012. Of these women, 832 women were included in this retrospective study. The mean patient age was 45.4 ± 13.7 years (range:15-80). The distribution of Pap smear results was normal (4.7%), atypical squamous cells of uncertain significance (ASCUS) (42.1%), low-grade squamous intraepithelial lesion (26.8%), ASC-H (7.0%), and high-grade squamous intraepithelial lesion (HSIL) (19.5%). In women with ASCUS, none of the 87 HPV-negative had ≥cervical intraepithelial neoplasia (CIN2) (P < 0.001). In women with ASC-H, only one out of 17 HPV-negative vs. 14 out of 41 HPV-positive had ≥CIN2 (P = 0.025). In patients with HSIL, 54.5% of HPV-negative had ≥CIN2, as compared to 80.8% of HPV-positive with ≥CIN2 (P = 0.039). Patients were further analyzed by age groups: <30 and ≥30 years. In HPV-negative women, there was a significant difference in the ratio of ≥CIN2 (30.8% <30 vs. 4.5% ≥30, P = 0.005). When the HPV DNA test was negative in women ≥30, the risk of ≥CIN2 was significantly lower (P < 0.001). HPV DNA cotesting in women with ASCUS and ASC-H furnish healthcare providers with informative data. There is a lower proportion of ≥CIN2 in HPV-negative women and a higher proportion of ≥CIN2 in HPV-positive. When HPV data were further evaluated by age group, the risk of ≥CIN2 was lower in HPV-negative women, especially in women ≥30. © 2014 Wiley Periodicals, Inc.
Upper crustal structure in Puget Lowland, Washington: Results from the 1998 Seismic Hazards Investigation in Puget Sound

USGS Publications Warehouse

Brocher, T.M.; Parsons, T.; Blakely, R.J.; Christensen, N.I.; Fisher, M.A.; Wells, R.E.; ten Brink, Uri S.; Pratt, T.L.; Crosson, R.S.; Creager, K.C.; Symons, N.P.; Preston, L.A.; Van Wagoner, T.; Miller, K.C.; Snelson, C.M.; Trehu, A.M.; Langenheim, V.E.; Spence, G.D.; Ramachandran, K.; Hyndman, R.A.; Mosher, D.C.; Zelt, B.C.; Weaver, C.S.

2001-01-01

A new three-dimensional (3-D) model shows seismic velocities beneath the Puget Lowland to a depth of 11 km. The model is based on a tomographic inversion of nearly one million first-arrival travel times recorded during the 1998 Seismic Hazards Investigation in Puget Sound (SHIPS), allowing higher-resolution mapping of subsurface structures than previously possible. The model allows us to refine the subsurface geometry of previously proposed faults (e.g., Seattle, Hood Canal, southern Whidbey Island, and Devils Mountain fault zones) as well as to identify structures (Tacoma, Lofall, and Sequim fault zones) that warrant additional study. The largest and most important of these newly identified structures lies along the northern boundary of the Tacoma basin; we informally refer to this structure here as the Tacoma fault zone. Although tomography cannot provide information on the recency of motion on any structure, Holocene earthquake activity on the Tacoma fault zone is suggested by seismicity along it and paleoseismic evidence for abrupt uplift of tidal marsh deposits to its north. The tomography reveals four large, west to northwest trending low-velocity basins (Tacoma, Seattle, Everett, and Port Townsend) separated by regions of higher velocity ridges that are coincident with fault-bounded uplifts of Eocene Crescent Formation basalt and pre-Tertiary basement. The shapes of the basins and uplifts are similar to those observed in gravity data; gravity anomalies calculated from the 3-D tomography model are in close agreement with the observed anomalies. In velocity cross sections the Tacoma and Seattle basins are asymmetric: the basin floor dips gently toward a steep boundary with the adjacent high-velocity uplift, locally with a velocity "overhang" that suggests a basin vergent thrust fault boundary. Crustal fault zones grow from minor folds into much larger structures along strike. Inferred structural relief across the Tacoma fault zone increases by several
Upper crustal structure in Puget Lowland, Washington: Results from the 1998 Seismic Hazards Investigation in Puget Sound

NASA Astrophysics Data System (ADS)

Brocher, Thomas M.; Parsons, Tom; Blakely, Richard J.; Christensen, Nikolas I.; Fisher, Michael A.; Wells, Ray E.

2001-01-01

A new three-dimensional (3-D) model shows seismic velocities beneath the Puget Lowland to a depth of 11 km. The model is based on a tomographic inversion of nearly one million first-arrival travel times recorded during the 1998 Seismic Hazards Investigation in Puget Sound (SHIPS), allowing higher-resolution mapping of subsurface structures than previously possible. The model allows us to refine the subsurface geometry of previously proposed faults (e.g., Seattle, Hood Canal, southern Whidbey Island, and Devils Mountain fault zones) as well as to identify structures (Tacoma, Lofall, and Sequim fault zones) that warrant additional study. The largest and most important of these newly identified structures lies along the northern boundary of the Tacoma basin; we informally refer to this structure here as the Tacoma fault zone. Although tomography cannot provide information on the recency of motion on any structure, Holocene earthquake activity on the Tacoma fault zone is suggested by seismicity along it and paleoseismic evidence for abrupt uplift of tidal marsh deposits to its north. The tomography reveals four large, west to northwest trending low-velocity basins (Tacoma, Seattle, Everett, and Port Townsend) separated by regions of higher velocity ridges that are coincident with fault-bounded uplifts of Eocene Crescent Formation basalt and pre-Tertiary basement. The shapes of the basins and uplifts are similar to those observed in gravity data; gravity anomalies calculated from the 3-D tomography model are in close agreement with the observed anomalies. In velocity cross sections the Tacoma and Seattle basins are asymmetric: the basin floor dips gently toward a steep boundary with the adjacent high-velocity uplift, locally with a velocity "overhang" that suggests a basin vergent thrust fault boundary. Crustal fault zones grow from minor folds into much larger structures along strike. Inferred structural relief across the Tacoma fault zone increases by several
Persistent carry-over effects of planktonic exposure to ocean acidification in the Olympia oyster.

PubMed

Hettinger, Annaliese; Sanford, Eric; Hill, Tessa M; Russell, Ann D; Sato, Kirk N S; Hoey, Jennifer; Forsch, Margaux; Page, Heather N; Gaylord, Brian

2012-12-01

Predicting impacts of global environmental change is challenging due to the complex life cycles that characterize many terrestrial and aquatic taxa. Different life stages often interact with the physical environment in distinct ways, and a growing body of work suggests that stresses experienced during one life stage can "carry over" to influence subsequent stages. Assessments of population responses to environmental perturbation must therefore consider how effects might propagate across life-history transitions. We investigated consequences of ocean acidification (decreased pH and carbonate saturation) for early life stages of the Olympia oyster (Ostrea lurida), a foundation species in estuaries along the Pacific coast of North America. We reared oysters at three levels of seawater pH, including a control (8.0) and two additional levels (7.9 and 7.8). Oysters were cultured through their planktonic larval period to metamorphosis and into early juvenile life. Larvae reared under pH 7.8 exhibited a 15% decrease in larval shell growth rate, and a 7% decrease in shell area at settlement, compared to larvae reared under control conditions. Impacts were even more pronounced a week after settlement, with juveniles that had been reared as larvae under reduced pH exhibiting a 41% decrease in shell growth rate. Importantly, the latter effect arose regardless of the pH level the oysters experienced as juveniles, indicating a strong carry-over effect from the larval phase. Adverse impacts of early exposure to low pH persisted for at least 1.5 months after juveniles were transferred to a common environment. Overall, our results suggest that a stringent focus on a single phase of the life cycle (e.g., one perceived as the "weakest link") may neglect critical impacts that can be transferred across life stages in taxa with complex life histories.
Adipose derived stem cells (ASCs) isolated from breast cancer tissue express IL-4, IL-10 and TGF-β1 and upregulate expression of regulatory molecules on T cells: do they protect breast cancer cells from the immune response?

PubMed

Razmkhah, Mahboobeh; Jaberipour, Mansooreh; Erfani, Nasrollah; Habibagahi, Mojtaba; Talei, Abdol-rasoul; Ghaderi, Abbas

2011-01-01

Immunomodulatory function of bone marrow derived mesenchymal stem cells in cancer has recently been investigated. But the resident mesenchymal stem cells as whole in cancer and in the breast cancer tissue have not been studied well. In the present work we isolated adipose derived stem cells (ASCs) from breast cancer and normal breast tissues to investigate the expressions of IL-4, IL-10 and transforming growth factor (TGF)-β1 in ASCs and to see if ASCs isolated from patients can modulate the regulatory molecules on peripheral blood lymphocytes. Our results showed that IL-10 and TGF-β1 have significantly higher mRNA expressions in ASCs isolated from breast cancer patients than those from normal individuals (P value <0.05). The culture supernatant of ASCs isolated from breast cancer patients with pathological stage III induced upregulation of the mRNA expression levels of IL-4, TGF-β1, IL-10, CCR4 and CD25 in PBLs. In addition, the percentage of CD4+CD25(high)Foxp3(+) T regulatory cells was increased in vitro. When the same culture supernatant was added to ASCs isolated from normal subjects augmentation of the mRNA expressions of IL-4, IL-10, IL-8, MMP2, VEGF and SDF-1 in normal ASCs was also observed. These data collectively conclude that resident ASCs in breast cancer tissue may have crucial roles in breast tumor growth and progression by inducing regulatory molecules and promoting anti-inflammatory reaction within the tumor microenvironment. Further investigation is required to see if the immune suppression induced by ASCs is an independent property from tumor cells or ASCs gain their immunosuppressive potential from malignant cells. Copyright © 2010 Elsevier Inc. All rights reserved.
Effects of Metal Micro and Nano-Particles on hASCs: An In Vitro Model.

PubMed

Palombella, Silvia; Pirrone, Cristina; Rossi, Federica; Armenia, Ilaria; Cherubino, Mario; Valdatta, Luigi; Raspanti, Mario; Bernardini, Giovanni; Gornati, Rosalba

2017-08-03

As the knowledge about the interferences of nanomaterials on human staminal cells are scarce and contradictory, we undertook a comparative multidisciplinary study based on the size effect of zero-valent iron, cobalt, and nickel microparticles (MPs) and nanoparticles (NPs) using human adipose stem cells (hASCs) as a model, and evaluating cytotoxicity, morphology, cellular uptake, and gene expression. Our results suggested that the medium did not influence the cell sensitivity but, surprisingly, the iron microparticles (FeMPs) resulted in being toxic. These data were supported by modifications in mRNA expression of some genes implicated in the inflammatory response. Microscopic analysis confirmed that NPs, mainly internalized by endocytosis, persist in the vesicles without any apparent cell damage. Conversely, MPs are not internalized, and the effects on hASCs have to be ascribed to the release of ions in the culture medium, or to the reduced oxygen and nutrient exchange efficiency due to the presence of MP agglomerating around the cells. Notwithstanding the results depicting a heterogeneous scene that does not allow drawing a general conclusion, this work reiterates the importance of comparative investigations on MPs, NPs, and corresponding ions, and the need to continue the thorough verification of NP and MP innocuousness to ensure unaffected stem cell physiology and differentiation.
The Accuracy of p16/Ki-67 and HPV Test in the Detection of CIN2/3 in Women Diagnosed with ASC-US or LSIL

PubMed Central

Possati-Resende, Júlio C.; Fregnani, José H. T. G.; Kerr, Ligia M.; Mauad, Edmundo C.; Longatto-Filho, Adhemar; Scapulatempo-Neto, Cristovam

2015-01-01

The objective of this study was to compare the accuracies of double staining for p16/Ki-67 and the molecular test for high-risk HPV (hr-HPV) to identify high-grade cervical intraepithelial neoplasia (CIN2/CIN3) in women with cervical cytology of atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL). Data were collected from 201 women who underwent cervical cytology screening in the Barretos Cancer Hospital and their results were categorized as ASC-US (n=96) or LSIL (n=105). All patients underwent colposcopy with or without cervical biopsy for diagnosis of CIN2/CIN3. The hr-HPV test (Cobas 4800 test) and immunocytochemistry were performed to detect biomarkers p16/Ki-67 (CINtec PLUS test). Two samples (1 ASC-US/1 LSIL) were excluded from the analysis due to inconclusive results of the histologic examination. There were 8 cases of CIN2/CIN3 among 95 women with ASC-US (8.4%), and 23 cases of CIN2/CIN3 among 104 women with LSIL (22.1%). In the group of women with ASC-US, the sensitivity and specificity in diagnosing CIN2/CIN3 were 87.5% and 79.5% for the HPV test and 62.5% and 93.1% for p16/Ki-67. Among women with LSIL, the sensitivity and specificity in the diagnosis of CIN2/CIN3 were 87% and 34.7% for the HPV test and 69.6% and 75.3% for immunocytochemistry. Superior performance was observed for p16/Ki-67 double staining, especially among women under 30 for whom the test had an area under the ROC curve of 0.762 (p<0.001). Both p16/Ki-67 double staining and the hr-HPV DNA test had similar performance in predicting high-grade cervical intraepithelial neoplasia among women with ASC-US. The best performance was observed in women aged >30 years. In younger women (≤30 years) with LSIL, p16/Ki-67 had greater accuracy in identifying precursor lesions. Among women >30 years diagnosed with LSIL, the two methods showed similar performance. PMID:26230097
Puget Lowland Ecoregion: Chapter 2 in Status and trends of land change in the Western United States--1973 to 2000

USGS Publications Warehouse

Sorenson, Daniel G.

2012-01-01

vegetation zone is named after the western hemlock (Tsuga heterophylla), Douglas-fir (Pseudotsuga menziesii) is the dominant tree species. Seattle, which had an estimated population of 563,376 in 2000, is the largest city in the Puget Lowland Ecoregion (Puget Sound Regional Council, 2001). The greater Seattle metropolitan area, comprising Seattle, Tacoma, Bellevue, and Bremerton, had an estimated population of 3.5 million people in 2000 (U.S. Census Bureau, 2000). Other sizable cities in the ecoregion include the state capital Olympia, as well as Tacoma, Bellingham, and Everett, Washington. The center of the Puget Lowland Ecoregion is dominated by the Seattle metropolitan area and developed land cover, whereas agriculture occurs mainly on river floodplains in the north and south. The remainder of the ecoregion area is dominated by forest land cover (fig. 1).
Triage of ASC-H: a meta-analysis of the accuracy of hrHPV testing and other markers to detect cervical precancer

PubMed Central

Xu, Lan; Verdoodt, Freija; Wentzensen, Nicolas; Bergeron, Christine; Arbyn, Marc

2015-01-01

Background Women with a cytological diagnosis of atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H) are usually immediately referred to colposcopy. However, triage may reduce the burden of diagnostic work-up and avoid over-treatment. Methods A meta-analysis was conducted to assess the accuracy of hrHPV testing, and testing for other molecular markers to detect CIN of grade II or III or worse (CIN2+ or CIN3+) in women with ASC-H. An additional question assessed was whether triage is useful given the relatively high pre-triage probability of underlying precancer. Results The pooled absolute sensitivity and specificity for CIN2+ of HC2 (derived from 19 studies) was 93% (95% CI: 89–95%) and 45% (95% CI: 41–50%), respectively. The p16INK4a staining (only 3 studies) has similar sensitivity (93%, 95% CI:75–100%) but superior specificity (specificity ratio: 1.69) to HC2 for CIN2+. Testing for PAX1 gene methylation (only 1 study) showed a superior specificity of 95% (specificity ratio: 2.08). The average pre-test risk was 34% for CIN2+ and 20% for CIN3+. A negative HC2 result decreased this to 8% and 5%, whereas a positive result upgraded the risk to 47% and 28%. Conclusions Due to the high probability of precancer in ASC-H, the utility of triage is limited. The usual recommendation to refer women with ASC-H to colposcopy is not altered by a positive triage test, whatever the test used. A negative hrHPV DNA or p16INK4a test may allow for repeat testing but this recommendation will depend on local decision thresholds for referral. PMID:26618614
The cytokine release inhibitory drug CRID3 targets ASC oligomerisation in the NLRP3 and AIM2 inflammasomes.

PubMed

Coll, Rebecca C; Robertson, Avril; Butler, Mark; Cooper, Matthew; O'Neill, Luke A J

2011-01-01

The Inflammasomes are multi-protein complexes that regulate caspase-1 activation and the production of the pro-inflammatory cytokine IL-1β. Previous studies identified a class of diarylsulfonylurea containing compounds called Cytokine Release Inhibitory Drugs (CRIDs) that inhibited the post-translational processing of IL-1β. Further work identified Glutathione S-Transferase Omega 1 (GSTO1) as a possible target of these CRIDs. This study aimed to investigate the mechanism of the inhibitory activity of the CRID CP-456,773 (termed CRID3) in light of recent advances in the area of inflammasome activation, and to clarify the potential role of GSTO1 in the regulation of IL-1β production. In murine bone marrow derived macrophages, CRID3 inhibited IL-1β secretion and caspase 1 processing in response to stimulation of NLRP3 and AIM2 but not NLRC4. CRID3 also prevented AIM2 dependent pyroptosis in contrast to the NLRP3 inhibitors glyburide and parthenolide, which do not inhibit AIM2 activation. Confocal microscopy and Western blotting assays indicated that CRID3 inhibited the formation of ASC complexes or 'specks' in response to NLRP3 and AIM2 stimulation. Co-immunoprecipitation assays show that GSTO1 interacted with ASC. These results identify CRID3 as a novel inhibitor of the NLRP3 and AIM2 inflammasomes and provide an insight into the mechanism of action of this small molecule. In addition GSTO1 may be a component of the inflammasome that is required for ASC complex formation. © 2011 Coll, O’Neill.
Triage of women with atypical squamous cells of undetermined significance (ASC-US): results of an Italian multicentric study.

PubMed

Del Mistro, Annarosa; Frayle-Salamanca, Helena; Trevisan, Rossana; Matteucci, Mario; Pinarello, Antonella; Zambenedetti, Pamela; Buoso, Rita; Fantin, Gian Piero; Zorzi, Manuel; Minucci, Daria

2010-04-01

To compare the performance of immediate colposcopy, repeat Pap test and HPV test as triage options for women diagnosed as having atypical squamous cells of undetermined significance (ASC-US) while attending organised screening for cervical carcinoma in five centres of the Veneto region. Women consecutively diagnosed as having ASC-US were included in a prospective study, and underwent colposcopy and collection of cervico-vaginal cells for conventional Pap test and HPV test (Hybrid Capture 2, High-risk probe set, Digene). Repetition of all three tests was scheduled for 12 months later. DNA was subsequently extracted from residual cells of positive samples, and analysed by polymerase chain reaction with several primers for typing of HPV sequences. Sensitivity, specificity and positive predictive value (PPV) of the different triage options for histology-confirmed cervical intraepithelial neoplasia, grade 2 or worse (CIN2+) were calculated among all women and by age (under and above 35 years). Seven hundred forty-nine women 25-64 years old (median age 42 years) were enrolled in the study. Pap smears at enrolment were read as ASC-US or more severe in 211 (29.4%) cases, colposcopy disclosed an atypical transformation zone in 254 (34.2%) women, and HPV test was positive in 181 (24.2%). High-grade cervical lesions developed in 29/749 (3.9%) women. HPV typing was possible in 163 (90%) of the samples, and carcinogenic types were present in 123. HPV test showed the best performance; overall, it had the highest sensitivity (92.3%), specificity (78.6%) and PPV (14.9%). Copyright 2009 Elsevier Inc. All rights reserved.
Comparison of lysimeter based and calculated ASCE reference evapotranspiration in a subhumid climate

NASA Astrophysics Data System (ADS)

Nolz, Reinhard; Cepuder, Peter; Eitzinger, Josef

2016-04-01

The standardized form of the well-known FAO Penman-Monteith equation, published by the Environmental and Water Resources Institute of the American Society of Civil Engineers (ASCE-EWRI), is recommended as a standard procedure for calculating reference evapotranspiration (ET ref) and subsequently plant water requirements. Applied and validated under different climatic conditions it generally achieved good results compared to other methods. However, several studies documented deviations between measured and calculated reference evapotranspiration depending on environmental and weather conditions. Therefore, it seems generally advisable to evaluate the model under local environmental conditions. In this study, reference evapotranspiration was determined at a subhumid site in northeastern Austria from 2005 to 2010 using a large weighing lysimeter (ET lys). The measured data were compared with ET ref calculations. Daily values differed slightly during a year, at which ET ref was generally overestimated at small values, whereas it was rather underestimated when ET was large, which is supported also by other studies. In our case, advection of sensible heat proved to have an impact, but it could not explain the differences exclusively. Obviously, there were also other influences, such as seasonal varying surface resistance or albedo. Generally, the ASCE-EWRI equation for daily time steps performed best at average weather conditions. The outcomes should help to correctly interpret ET ref data in the region and in similar environments and improve knowledge on the dynamics of influencing factors causing deviations.
3D Topography of the Young Adult Anal Sphincter Complex Reconstructed from Undeformed Serial Anatomical Sections

PubMed Central

Wu, Yi; Dabhoiwala, Noshir F.; Hagoort, Jaco; Shan, Jin-Lu; Tan, Li-Wen; Fang, Bin-Ji; Zhang, Shao-Xiang; Lamers, Wouter H.

2015-01-01

Background Pelvic-floor anatomy is usually studied by artifact-prone dissection or imaging, which requires prior anatomical knowledge. We used the serial-section approach to settle contentious issues and an interactive 3D-pdf to make the results widely accessible. Method 3D reconstructions of undeformed thin serial anatomical sections of 4 females and 2 males (21–35y) of the Chinese Visible Human database. Findings Based on tendinous septa and muscle-fiber orientation as segmentation guides, the anal-sphincter complex (ASC) comprised the subcutaneous external anal sphincter (EAS) and the U-shaped puborectal muscle, a part of the levator ani muscle (LAM). The anococcygeal ligament fixed the EAS to the coccygeal bone. The puborectal-muscle loops, which define the levator hiatus, passed around the anorectal junction and inserted anteriorly on the perineal body and pubic bone. The LAM had a common anterior attachment to the pubic bone, but separated posteriorly into puborectal and “pubovisceral” muscles. This pubovisceral muscle was bilayered: its internal layer attached to the conjoint longitudinal muscle of the rectum and the rectococcygeal fascia, while its outer, patchy layer reinforced the inner layer. ASC contraction makes the ano-rectal bend more acute and lifts the pelvic floor. Extensions of the rectal longitudinal smooth muscle to the coccygeal bone (rectococcygeal muscle), perineal body (rectoperineal muscle), and endopelvic fascia (conjoint longitudinal and pubovisceral muscles) formed a “diaphragm” at the inferior boundary of the mesorectum that suspended the anorectal junction. Its contraction should straighten the anorectal bend. Conclusion The serial-section approach settled contentious topographic issues of the pelvic floor. We propose that the ASC is involved in continence and the rectal diaphragm in defecation. PMID:26305117
9. Photocopy of Blueprint (Original held by the Community end ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

9. Photocopy of Blueprint (Original held by the Community end Economic Development Department, City of Detroit), C. Howard Crane, Architect. CROSS SECTION THROUGH STADIUM, NOVEMBER 27, 1926. (4' x 5' negative) - Olympia Arena, 5920 Grand River Avenue, Detroit, MI
Fitting and Modeling in the ASC Data Analysis Environment

NASA Astrophysics Data System (ADS)

Doe, S.; Siemiginowska, A.; Joye, W.; McDowell, J.

As part of the AXAF Science Center (ASC) Data Analysis Environment, we will provide to the astronomical community a Fitting Application. We present a design of the application in this paper. Our design goal is to give the user the flexibility to use a variety of optimization techniques (Levenberg-Marquardt, maximum entropy, Monte Carlo, Powell, downhill simplex, CERN-Minuit, and simulated annealing) and fit statistics (chi (2) , Cash, variance, and maximum likelihood); our modular design allows the user easily to add their own optimization techniques and/or fit statistics. We also present a comparison of the optimization techniques to be provided by the Application. The high spatial and spectral resolutions that will be obtained with AXAF instruments require a sophisticated data modeling capability. We will provide not only a suite of astronomical spatial and spectral source models, but also the capability of combining these models into source models of up to four data dimensions (i.e., into source functions f(E,x,y,t)). We will also provide tools to create instrument response models appropriate for each observation.
Semi-quantitative HPV viral load in patients with ASC-US cytology: viral load correlates strongly with the presence of CIN but only weakly with its severity.

PubMed

Lee, S J; Kim, W Y; Shim, S-H; Cho, S-H; Oh, I K; Hwang, T S; Kim, S-N; Kang, S-B

2015-02-01

This study was performed to evaluate the prognostic significance of human papillomavirus (HPV) viral load, expressed in relative light units (RLUs), in patients with atypical squamous cells of undetermined significance (ASC-US) cytology. A total of 349 ASC-US cases with HPV infection, detected using Hybrid Capture 2, were diagnosed histologically. A colposcopically directed punch biopsy was performed on acetowhite areas. Endocervical curettage biopsy and random cervical punch biopsy in four quadrants were performed in unsatisfactory colposcopy cases. In negative colposcopy cases, random cervical punch biopsy in four quadrants was performed. Case with no cervical intraepithelial neoplasia (CIN), CIN1 and CIN2+ (CIN2/CIN3) accounted for 162, 135 and 52 cases, respectively. The mean age showed no difference among the three groups (P = 0.510). There was a significant correlation between RLU values and the presence of CIN (P < 0.001), but less so with its severity: the median RLU values for negative, CIN1 and CIN2+ cases were 42.68, 146.45 and 156.43, respectively, with widely overlapping confidence intervals. The cut-off values of RLU to detect CIN1+ and CIN2+ were 6.73 and 45.64, respectively. The HPV viral load in ASC-US cases showed a significant correlation with the presence of CIN and less so with its severity, and showed large overlap of viral loads between grades of CIN. In ASC-US cases, RLU was not an accurate predictor of immediate high-grade CIN. © 2014 John Wiley & Sons Ltd.
Analysis of the Material Properties of Early Chondrogenic Differentiated Adipose-Derived Stromal Cells (ASC) Using an in vitro Three-dimensional Micromass Culture System

PubMed Central

Xu, Yue; Balooch, Guive; Chiou, Michael; Bekerman, Elena; Ritchie, Robert O.; Longaker, Michael T.

2009-01-01

Cartilage is an avascular tissue with only a limited potential to heal and chondrocytes in vitro have poor proliferative capacity. Recently, adipose-derived stromal cells (ASC) have demonstrated a great potential for application to tissue engineering due to their ability to differentiate into cartilage, bone, and fat. In this study, we have utilized a high density three-dimensional (3D) micromass model system of early chondrogenesis with ASC. The material properties of these micromasses showed a significant increase in dynamic and static elastic modulus during the early chondrogenic differentiation process. These data suggest that the 3D micromass culture system represents an in vitro model of early chondrogenesis with dynamic cell signaling interactions associated with the mechanical properties of chondrocyte differentiation. PMID:17543281
Analysis of the material properties of early chondrogenic differentiated adipose-derived stromal cells (ASC) using an in vitro three-dimensional micromass culture system

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xu, Yue; Balooch, Guive; Chiou, Michael

2007-07-27

Cartilage is an avascular tissue with only a limited potential to heal and chondrocytes in vitro have poor proliferative capacity. Recently, adipose-derived stromal cells (ASC) have demonstrated a great potential for application to tissue engineering due to their ability to differentiate into cartilage, bone, and fat. In this study, we have utilized a high density three-dimensional (3D) micromass model system of early chondrogenesis with ASC. The material properties of these micromasses showed a significant increase in dynamic and static elastic modulus during the early chondrogenic differentiation process. These data suggest that the 3D micromass culture system represents an in vitromore » model of early chondrogenesis with dynamic cell signaling interactions associated with the mechanical properties of chondrocyte differentiation.« less
Comparison of sum-of-hourly and daily time step standardized ASCE Penman-Monteith reference evapotranspiration

NASA Astrophysics Data System (ADS)

Djaman, Koffi; Irmak, Suat; Sall, Mamadou; Sow, Abdoulaye; Kabenge, Isa

2017-10-01

The objective of this study was to quantify differences associated with using 24-h time step reference evapotranspiration (ETo), as compared with the sum of hourly ETo computations with the standardized ASCE Penman-Monteith (ASCE-PM) model for semi-arid dry conditions at Fanaye and Ndiaye (Senegal) and semiarid humid conditions at Sapu (The Gambia) and Kankan (Guinea). The results showed that there was good agreement between the sum of hourly ETo and daily time step ETo at all four locations. The daily time step overestimated the daily ETo relative to the sum of hourly ETo by 1.3 to 8% for the whole study periods. However, there is location and monthly dependence of the magnitude of ETo values and the ratio of the ETo values estimated by both methods. Sum of hourly ETo tends to give higher ETo during winter time at Fanaye and Sapu, while the daily ETo was higher from March to November at the same weather stations. At Ndiaye and Kankan, daily time step estimates of ETo were high during the year. The simple linear regression slopes between the sum of 24-h ETo and the daily time step ETo at all weather stations varied from 1.02 to 1.08 with high coefficient of determination (R 2 ≥ 0.87). Application of the hourly ETo estimation method might help on accurate ETo estimation to meet irrigation requirement under precision agriculture.

New Whole-House Solutions Case Study: Testing Ductless Heat Pumps in High-Performance Affordable Housing, the Woods at Golden Given - Tacoma, Washington

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

2015-06-01

The Woods is a 30-home, high- performance, energy efficient sustainable community built by Habitat for Humanity (HFH). With Support from Tacoma Public Utilities, Washington State University (part of the Building America Partnership for Improved Residential Construction) is researching the energy performance of these homes and the ductless heat pumps (DHP) they employ. This project provides Building America with an opportunity to: field test HVAC equipment, ventilation system air flows, building envelope tightness, lighting, appliance, and other input data that are required for preliminary Building Energy Optimization (BEopt™) modeling and ENERGY STAR® field verification; analyze cost data from HFH and othermore » sources related to building-efficiency measures that focus on the DHP/hybrid heating system and heat recovery ventilation system; evaluate the thermal performance and cost benefit of DHP/hybrid heating systems in these homes from the perspective of homeowners; compare the space heating energy consumption of a DHP/electric resistance (ER) hybrid heating system to that of a traditional zonal ER heating system; conduct weekly "flip-flop tests" to compare space heating, temperature, and relative humidity in ER zonal heating mode to DHP/ER mode.« less
DoD Electronic Data Interchange (EDI) Convention: ASC X12 Transaction Set 832 Price Sales Catalog (Version 003030)

DTIC Science & Technology

1992-12-01

DATA DES . ELEMENT NAME ATlNPUTES Conditional TD401 152 Special Handling Code C ID 2/3 Code specifying special transportation handling instructions. HAN...Executhre Age"t for Eketronic Conmnerce/Electmnlc Dots lnterchange/Protection of Logistica Undaasslfled/Serssltlve Systerr Executive Agent for EC/EDI...PRICEISALES CATALOG ANSI ASC X12 VERSIONIRELEASE 003030DOD_ 7 Communications Transport Protocol ISA /_Interchange Control Header GS/ Functional Group Header
DoD Electronic Data Interchange (EDI) Convention: ASC X12 Transaction Set 859 Generic Freight Invoice (Version 003020)

DTIC Science & Technology

1993-04-01

FREIGHT INVOICE (VERSION 003020) FORMATTING INVOICE INFORMATION FOR THE DoD TRANSPORTATION PAYMENT SYSTEM USING THE X1 2.55 TRANSACTION SET 859 GENERIC...GBYERIC FREIGHT NIVOICE EDI CONVENTON 859.003020 * Contents FORMATTING INVOICE INFORMATION FOR THE DoD TRANSPORTATION PAYMENT SYSTEM USING THE Xl 2.55... transportation invoice using the ASC X12.55 Transaction Set 859 Generic Freight Invoice (003020). It contains information for the design of interface
4. Photocopy of a 1943 architectural drawing titled: 'Patients & ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. Photocopy of a 1943 architectural drawing titled: 'Patients & Detachment Mess, MESS-Z-H. Elevations & Sections.' 1-13-43 - Madigan Hospital, Patients' & Medical Detachments, Bounded by Wilson & McKinley Avenues & Garfield & Lincoln Streets, Tacoma, Pierce County, WA
Code Verification Results of an LLNL ASC Code on Some Tri-Lab Verification Test Suite Problems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Anderson, S R; Bihari, B L; Salari, K

As scientific codes become more complex and involve larger numbers of developers and algorithms, chances for algorithmic implementation mistakes increase. In this environment, code verification becomes essential to building confidence in the code implementation. This paper will present first results of a new code verification effort within LLNL's B Division. In particular, we will show results of code verification of the LLNL ASC ARES code on the test problems: Su Olson non-equilibrium radiation diffusion, Sod shock tube, Sedov point blast modeled with shock hydrodynamics, and Noh implosion.
DoD Electronic Data Interchange (EDI) Convention: ASC X12 Transaction Set 856 Ship Notice/Manifest (Version 003030)

DTIC Science & Technology

1993-01-01

from the Department of Defense Ecutive Agent for Elctronic Com••rce/Electronic Data interrrhange/Ped~on of Logisti Unc.iaassfed/fen-une Systers...Convention: Electronic Commerce ; ANSI X12, X12; 62 electronic standards, electronic business standards; computer-to-computer exchange of data...Ship Notice Manifest Information From Invoicng Party to DFAS Using ASC X12 856A 10.1) Reserved 10. E Reserved CV QUAMY Tr7 4 10O.F Reserved__
Elimination of the NLRP3-ASC Inflammasome Protects against Chronic Obesity-Induced Pancreatic Damage

PubMed Central

Youm, Yun-Hee; Adijiang, Ayinuer; Vandanmagsar, Bolormaa; Burk, David; Ravussin, Anthony

2011-01-01

Clinical evidence that the blockade of IL-1β in type-2 diabetic patients improves glycemia is indicative of an autoinflammatory mechanism that may trigger adiposity-driven pancreatic damage. IL-1β is a key contributor to the obesity-induced inflammation and subsequent insulin resistance, pancreatic β-cell dysfunction, and the onset of type 2 diabetes. Our previous studies demonstrated that the ceramides activate the Nod-like receptor family, pyrin domain containing 3 (Nlrp3) inflammasome to cause the generation of mature IL-1β and ablation of the Nlrp3 inflammasome in diet-induced obesity improves insulin signaling. However, it remains unclear whether the posttranslational processing of active IL-1β in pancreas is regulated by the NLRP3 inflammasome or whether the alternate mechanisms play a dominant role in chronic obesity-induced pancreatic β-cell exhaustion. Here we show that loss of ASC, a critical adaptor required for the assembly of the NLRP3 and absent in melanoma 2 inflammasome substantially improves the insulin action. Surprisingly, despite lower insulin resistance in the chronically obese NLRP3 and ASC knockout mice, the insulin levels were substantially higher when the inflammasome pathway was eliminated. The obesity-induced increase in maturation of pancreatic IL-1β and pancreatic islet fibrosis was dependent on the NLRP3 inflammasome activation. Furthermore, elimination of NLRP3 inflammasome protected the pancreatic β-cells from cell death caused by long-term high-fat feeding during obesity with significant increase in the size of the islets of Langerhans. Collectively, this study provides direct in vivo evidence that activation of the NLRP3 inflammasome in diet-induced obesity is a critical trigger in causing pancreatic damage and is an important mechanism of progression toward type 2 diabetes. PMID:21862613
77 FR 53772 - Approval and Promulgation of Air Quality Implementation Plans; Washington; Determination of Clean...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-04

... Fine Particulate Standard for the Tacoma, Pierce County Nonattainment Area AGENCY: Environmental..., Pierce County nonattainment area (hereafter referred to as ``Tacoma, Pierce County'' or ``the area'') has... the Tacoma, Pierce County nonattainment area has clean data for the 2006 24-hour PM 2.5 NAAQS. This...
Evaluation and Validation of Organic Materials for Advanced Stirling Convertors (ASCs): Overview

NASA Technical Reports Server (NTRS)

Shin, Euy-Sik Eugene

2015-01-01

Various organic materials are used as essential parts in Stirling Convertors for their unique properties and functionalities such as bonding, potting, sealing, thread locking, insulation, and lubrication. More efficient Advanced Stirling Convertors (ASC) are being developed for future space applications especially with a long mission cycle, sometimes up to 17 years, such as deep space exploration or lunar surface power or Mars rovers, and others. Thus, performance, durability, and reliability of those organics should be critically evaluated in every possible material-process-fabrication-service environment relations based on their mission specifications. In general, thermal stability, radiation hardness, outgassing, and material compatibility of the selected organics have been systematically evaluated while their process and fabrication conditions and procedures were being optimized. Service environment-simulated long term aging tests up to 4 years were performed as a function of temperature for durability assessment of the most critical organic material systems.
ASC-J9(®) suppresses castration resistant prostate cancer progression via degrading the enzalutamide-induced androgen receptor mutant AR-F876L.

PubMed

Wang, Ronghao; Lin, Wanying; Lin, Changyi; Li, Lei; Sun, Yin; Chang, Chawnshang

2016-08-28

Androgen deprivation therapy (ADT) with the newly developed powerful anti-androgen enzalutamide (Enz, also known as MDV3100) has promising therapeutic effects to suppress castration resistant prostate cancer (CRPC) and extending patients' lives an extra 4.8 months. However, most Enz therapy eventually fails with the development of Enz resistance. The detailed mechanisms how CRPC develops Enz resistance remain unclear and may involve multiple mechanisms. Among them, the induction of the androgen receptor (AR) mutant AR-F876L in some CRPC patients may represent one driving force that confers Enz resistance. Here, we demonstrate that the AR degradation enhancer, ASC-J9(®), not only degrades wild-type AR, but also has the ability to target AR-F876L. The consequence of suppressing AR-F876L may then abrogate AR-F876L mediated CRPC cell proliferation and metastasis. Thus, developing ASC-J9(®) as a new therapeutic approach may represent a novel therapy to better suppress CRPC that has already developed Enz resistance. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
32. VERTICAL OSCILLATIONS, 3/4 VIEW, 7 NOVEMBER 1940, FROM 16MM ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

32. VERTICAL OSCILLATIONS, 3/4 VIEW, 7 NOVEMBER 1940, FROM 16MM FILM SHOT PROFESSOR F.B. FARQUHARSON, UNIVERSITY OF WASHINGTON. (LABORATORY STUDIES ON THE TACOMA NARROWS BRIDGE, AT UNIVERSITY OF WASHINGTON (SEATTLE: UNIVERSITY OF WASHINGTON, DEPARTMENT OF CIVIL ENGINEERING, 1941) - Tacoma Narrows Bridge, Spanning Narrows at State Route 16, Tacoma, Pierce County, WA
Measurement of spatial and temporal variation in volatile hazardous air pollutants in Tacoma, Washington, using a mobile membrane introduction mass spectrometry (MIMS) system.

PubMed

Davey, Nicholas G; Fitzpatrick, Cole T E; Etzkorn, Jacob M; Martinsen, Morten; Crampton, Robert S; Onstad, Gretchen D; Larson, Timothy V; Yost, Michael G; Krogh, Erik T; Gilroy, Michael; Himes, Kathy H; Saganić, Erik T; Simpson, Christopher D; Gill, Christopher G

2014-09-19

The objective of this study was to use membrane introduction mass spectrometry (MIMS), implemented on a mobile platform, in order to provide real-time, fine-scale, temporally and spatially resolved measurements of several hazardous air pollutants. This work is important because there is now substantial evidence that fine-scale spatial and temporal variations of air pollutant concentrations are important determinants of exposure to air pollution and adverse health outcomes. The study took place in Tacoma, WA during periods of impaired air quality in the winter and summer of 2008 and 2009. Levels of fine particles were higher in winter compared to summer, and were spatially uniform across the study area. Concentrations of vapor phase pollutants measured by membrane introduction mass spectrometry (MIMS), notably benzene and toluene, had relatively uniform spatial distributions at night, but exhibited substantial spatial variation during the day-daytime levels were up to 3-fold higher at traffic-impacted locations compared to a reference site. Although no direct side-by-side comparison was made between the MIMS system and traditional fixed site monitors, the MIMS system typically reported higher concentrations of specific VOCs, particularly benzene, ethylbenzene and naphthalene, compared to annual average concentrations obtained from SUMA canisters and gas chromatographic analysis at the fixed sites.
34. VERTICAL AND TORSIONAL MOTION VIEWED FROM EAST TOWER, 7 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

34. VERTICAL AND TORSIONAL MOTION VIEWED FROM EAST TOWER, 7 NOVEMBER 1940, FROM 16MN FILM SHOT BY PROFESSOR F.B. FARQUHARSON, UNIVERSITY OF WASHINGTON. (LABORATORY STUDIES ON THE TACOMA NARROWS BRIDGE, AT UNIVERSITY OF WASHINGTON (SEATTLE: UNIVERSITY OF WASHINGTON, DEPARTMENT OF CIVIL ENGINEERING, 1941) - Tacoma Narrows Bridge, Spanning Narrows at State Route 16, Tacoma, Pierce County, WA
33. VERTICAL AND TORSIONAL OSCILLATIONS, 3/4 VIEW, 7 NOVEMBER 1940, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

33. VERTICAL AND TORSIONAL OSCILLATIONS, 3/4 VIEW, 7 NOVEMBER 1940, FROM 16MN FILM SHOT BY PROFESSOR F.B. FARQUHARSON, UNIVERSITY OF WASHINGTON. (LABORATORY STUDIES ON THE TACOMA NARROWS BRIDGE, AT UNIVERSITY OF WASHINGTON (SEATTLE: UNIVERSITY OF WASHINGTON, DEPARTMENT OF CIVIL ENGINEERING, 1941) - Tacoma Narrows Bridge, Spanning Narrows at State Route 16, Tacoma, Pierce County, WA
View of compartment C110, senior officers ward room from port ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View of compartment C-110, senior officers ward room from port to starboard, showing wooden furnishings, deck stanchions and the olympia's piano. (084) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
The Selective Centrifugation Ensures a Better In Vitro Isolation of ASCs and Restores a Soft Tissue Regeneration In Vivo

PubMed Central

De Francesco, Francesco; Guastafierro, Antonio; Nicoletti, Gianfranco; Razzano, Sergio; Riccio, Michele; Ferraro, Giuseppe A.

2017-01-01

Autologous fat grafting procedures in plastic surgery have been extensively used to reinforce soft tissue in congenital or acquired tissue impairments. With this background, the aim of this study is firstly to examine the impact of a selective centrifugation on existing adipose stem cells (ASCs) in terms of stemness profile maintenance and, secondly, to investigate the effect of restoring volume in reconstruction on patients affected by soft tissue damage. After centrifugation, the fat graft products were separated into two layers and subsequently examined in vitro for the expression of CD34, CD90, CD117, CD105, CD29, CD31, CD44, CD73, CD133, CD14 and CD45 markers by flow cytometry and gene expression analyses were performed for Sox2, WNT3A, END, CD44, FUT4, COLL1, CTNNB1, hbEGF, KRTLG, MMP2 and VIM genes. The results showed that in the middle-high density (MHD) layer there was a peak concentration of ASCs, compared to another layer obtained after centrifugation. Research carried out on patients under treatment for soft tissue regeneration using cells obtained from MHD layer selection will be fundamental in comparative analysis. These studies will lead to an adequate standardization of outcomes, provided that treatment is performed through cell selection. Therefore, a unique procedure in tissue reconstruction and regeneration through fat grafting is presented here. PMID:28498335
The Selective Centrifugation Ensures a Better In Vitro Isolation of ASCs and Restores a Soft Tissue Regeneration In Vivo.

PubMed

De Francesco, Francesco; Guastafierro, Antonio; Nicoletti, Gianfranco; Razzano, Sergio; Riccio, Michele; Ferraro, Giuseppe A

2017-05-12

Autologous fat grafting procedures in plastic surgery have been extensively used to reinforce soft tissue in congenital or acquired tissue impairments. With this background, the aim of this study is firstly to examine the impact of a selective centrifugation on existing adipose stem cells (ASCs) in terms of stemness profile maintenance and, secondly, to investigate the effect of restoring volume in reconstruction on patients affected by soft tissue damage. After centrifugation, the fat graft products were separated into two layers and subsequently examined in vitro for the expression of CD34, CD90, CD117, CD105, CD29, CD31, CD44, CD73, CD133, CD14 and CD45 markers by flow cytometry and gene expression analyses were performed for Sox2, WNT3A, END, CD44, FUT4, COLL1, CTNNB1, hbEGF, KRTLG, MMP2 and VIM genes. The results showed that in the middle-high density (MHD) layer there was a peak concentration of ASCs, compared to another layer obtained after centrifugation. Research carried out on patients under treatment for soft tissue regeneration using cells obtained from MHD layer selection will be fundamental in comparative analysis. These studies will lead to an adequate standardization of outcomes, provided that treatment is performed through cell selection. Therefore, a unique procedure in tissue reconstruction and regeneration through fat grafting is presented here.
77 FR 49857 - Early Scoping Notification for the Alternatives Analysis of the Tacoma Link Expansion in Tacoma, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-17

... requirements associated with the New Starts (``Section 5309'') funding program for certain kinds of major capital investments. While recent legislation may lead to changes in the New Starts process, Sound Transit... business and theater districts. Sound Move, the first phase of regional transit investments, was approved...
3. Ramp No. 6 connection between Medical Detachment Barracks: Building ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. Ramp No. 6 connection between Medical Detachment Barracks: Building Nos. 9970-B (left) and 9969-B (right). The many windows makes this section almost unique among the ramps and corridors. - Madigan Hospital, Corridors & Ramps, Bounded by Wilson & McKinley Avenues & Garfield & Lincoln Streets, Tacoma, Pierce County, WA
Correlation between gross anatomical topography, sectional sheet plastination, microscopic anatomy and endoanal sonography of the anal sphincter complex in human males

PubMed Central

Al-Ali, S; Blyth, P; Beatty, S; Duang, A; Parry, B; Bissett, I P

2009-01-01

This study elucidates the structure of the anal sphincter complex (ASC) and correlates the individual layers, namely the external anal sphincter (EAS), conjoint longitudinal muscle (CLM) and internal anal sphincter (IAS), with their ultrasonographic images. Eighteen male cadavers, with an average age of 72 years (range 62–82 years), were used in this study. Multiple methods were used including gross dissection, coronal and axial sheet plastination, different histological staining techniques and endoanal sonography. The EAS was a continuous layer but with different relations, an upper part (corresponding to the deep and superficial parts in the traditional description) and a lower (subcutaneous) part that was located distal to the IAS, and was the only muscle encircling the anal orifice below the IAS. The CLM was a fibro-fatty-muscular layer occupying the intersphincteric space and was continuous superiorly with the longitudinal muscle layer of the rectum. In its middle and lower parts it consisted of collagen and elastic fibres with fatty tissue filling the spaces between the fibrous septa. The IAS was a markedly thickened extension of the terminal circular smooth muscle layer of the rectum and it terminated proximal to the lower part of the EAS. On endoanal sonography, the EAS appeared as an irregular hyperechoic band; CLM was poorly represented by a thin irregular hyperechoic line and IAS was represented by a hypoechoic band. Data on the measurements of the thickness of the ASC layers are presented and vary between dissection and sonographic imaging. The layers of the ASC were precisely identified in situ, in sections, in isolated dissected specimens and the same structures were correlated with their sonographic appearance. The results of the measurements of ASC components in this study on male cadavers were variable, suggesting that these should be used with caution in diagnostic and management settings. PMID:19486204

Budget-impact model for colonoscopy cost calculation and comparison between 2 litre PEG+ASC and sodium picosulphate with magnesium citrate or sodium phosphate oral bowel cleansing agents.

PubMed

Gruss, H-J; Cockett, A; Leicester, R J

2012-01-01

With the availability of several bowel cleansing agents, physicians and hospitals performing colonoscopies will often base their choice of cleansing agent purely on acquisition cost. Therefore, an easy to use budget impact model has been developed and established as a tool to compare total colon preparation costs between different established bowel cleansing agents. The model was programmed in Excel and designed as a questionnaire evaluating information on treatment costs for a range of established bowel cleansing products. The sum of costs is based on National Health Service reference costs for bowel cleansing products. Estimations are made for savings achievable when using a 2-litre polyethylene glycol with ascorbate components solution (PEG+ASC) in place of other bowel cleansing solutions. Test data were entered into the model to confirm validity and sensitivity. The model was then applied to a set of audit cost data from a major hospital colonoscopy unit in the UK. Descriptive analysis of the test data showed that the main cost drivers in the colonoscopy process are the procedure costs and costs for bed days rather than drug acquisition costs, irrespective of the cleansing agent. Audit data from a colonoscopy unit in the UK confirmed the finding with a saving of £107,000 per year in favour of PEG+ASC when compared to sodium picosulphate with magnesium citrate solution (NaPic+MgCit). For every patient group the model calculated overall cost savings. This was irrespective of the higher drug expenditure associated with the use of PEG+ASC for bowel preparation. Savings were mainly realized through reduced costs for repeat colonoscopy procedures and associated costs, such as inpatient length of stay. The budget impact model demonstrated that the primary cost driver was the procedure cost for colonoscopy. Savings can be realized through the use of PEG+ASC despite higher drug acquisition costs relative to the comparator products. From a global hospital funding
Tutoring ESL: A Handbook for Volunteers.

ERIC Educational Resources Information Center

Reck, Deborah L.; And Others

This handbook is designed for use by Tacoma Community House volunteer tutors of English as a Second Language (ESL) as a supplement to basic volunteer training. The handbook includes detailed information in areas briefly covered during training and specific instructional ideas and class activities. A section on getting started discusses the…
78 FR 77548 - Noise Exposure Map Notice; Receipt of Noise Compatibility Program and Request for Review Seattle...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-23

...-Tacoma International Airport under the provisions of 49 U.S.C. 47501 et. seq. (Aviation Safety and Noise... comment. Under 49 U.S.C., Section 47503 (the Aviation Safety and Noise Abatement Act, (the Act), an... the evaluation process are whether the proposed measures may reduce the level of aviation safety...
35. VERTICAL AND TORSIONAL MOTION FROM EAST TOWER SHOWING ANGULAR ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

35. VERTICAL AND TORSIONAL MOTION FROM EAST TOWER SHOWING ANGULAR DISTORTION APPROACHING 45 DEGREES WITH LAMP POSTS APPEARING TO BE AT EIGHT ANGLES, 7 NOVEMBER 1940, FROM 16MN FILM SHOT BY PROFESSOR F.B. FARQUHARSON, UNIVERSITY OF WASHINGTON. (LABORATORY STUDIES ON THE TACOMA NARROWS BRIDGE, AT UNIVERSITY OF WASHINGTON SEATTLE: UNIVERSITY OF WASHINGTON, DEPARTMENT OF CIVIL ENGINEERING, 1941) - Tacoma Narrows Bridge, Spanning Narrows at State Route 16, Tacoma, Pierce County, WA
Spirulina platensis Improves Mitochondrial Function Impaired by Elevated Oxidative Stress in Adipose-Derived Mesenchymal Stromal Cells (ASCs) and Intestinal Epithelial Cells (IECs), and Enhances Insulin Sensitivity in Equine Metabolic Syndrome (EMS) Horses.

PubMed

Nawrocka, Daria; Kornicka, Katarzyna; Śmieszek, Agnieszka; Marycz, Krzysztof

2017-08-03

Equine Metabolic Syndrome (EMS) is a steadily growing life-threatening endocrine disorder linked to insulin resistance, oxidative stress, and systemic inflammation. Inflammatory microenvironment of adipose tissue constitutes the direct tissue milieu for various cell populations, including adipose-derived mesenchymal stromal cells (ASCs), widely considered as a potential therapeutic cell source in the course of the treatment of metabolic disorders. Moreover, elevated oxidative stress induces inflammation in intestinal epithelial cells (IECs)-the first-line cells exposed to dietary compounds. In the conducted research, we showed that in vitro application of Spirulina platensis contributes to the restoration of ASCs' and IECs' morphology and function through the reduction of cellular oxidative stress and inflammation. Enhanced viability, suppressed senescence, and improved proliferation of ASCs and IECs isolated from metabolic syndrome-affected individuals were evident following exposition to Spirulina. A protective effect of the investigated extract against mitochondrial dysfunction and degeneration was also observed. Moreover, our data demonstrate that Spirulina extract effectively suppressed LPS-induced inflammatory responses in macrophages. In vivo studies showed that horses fed with a diet based on Spirulina platensis supplementation lost weight and their insulin sensitivity improved. Thus, our results indicate the engagement of Spirulina platensis nourishing as an interesting alternative approach for supporting the conventional treatment of equine metabolic syndrome.
76 FR 63954 - Notice of Lodging of Proposed Consent Decree

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-14

... and Kimberly G. Smith, pursuant to Section 309(b) and (d) of the Clean Water Act, 33 U.S.C. 1319(b... violating the Clean Water Act by discharging pollutants without a permit into waters of the United States... for the Western District of Washington, 1717 Pacific Avenue, Room 3100, Tacoma, WA 98402-3200. In...
View forward of five inch gun mounted in sponson; note ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View forward of five inch gun mounted in sponson; note glass windows which would be removed in combat, gudgeons below window allowed attachment of armor plate which could be swung up to protect gun crew in battle; also note lower section of flag rack at top left center of photograph. (p39) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
Atypical Squamous Cells: Cytopathological Findings and Correlation with HPV Genotype and Histopathology.

PubMed

Oliveira, Geilson Gomes de; Oliveira, Judite Maria da Silva Costa de; Eleutério, Renata Mirian Nunes; Barbosa, Rita de Cássia Carvalho; Almeida, Paulo Roberto Carvalho de; Eleutério, José

2018-06-13

We aimed to assess potential associations between atypical squamous cell (ASC) subgroups: ASC-US (undetermined significance) and ASC-H (cannot exclude high-grade squamous intraepithelial lesion), regarding cytomorphological features, high-risk (HR) human papillomavirus (HPV) testing, and histological outcomes in a sample of Brazilian women. Cross-sectional study which evaluated 1,346 liquid-based cytologies between January 2010 and July 2016 with ASC results. ASC-US and ASC-H were analyzed for frequency, diagnostic criteria, and cytological findings and compared with HR-HPV tests and histological outcomes. Enlarged nucleus was the most frequent ASC-US criterion, but alternative criteria were present in 20% of the total cases. No ASC-US criteria were associated with histological outcomes or HR-HPV positivity. Parakeratosis, corneal pearl, giant cells, and binucleation were strongly associated with ASC-US while hyperkeratosis was associated with high-grade squamous intraepithelial lesions (HSIL) or a superior outcome. HR-HPV was positive in 64.39% of ASC-US and 65.38% of ASC-H. HSIL or superior outcomes also occurred in 13.33% of ASC-US and 64.71% of ASC-H cases. Alternative criteria for ASC-US were relatively frequent. Reactive cellular changes suggestive of atypias were more abundant in ASC-US. Although ASC-H is associated with worse histological outcomes, no differences in HPV positivity were found in comparison to ASC-US. © 2018 S. Karger AG, Basel.
Spatial and temporal variability of contaminants within estuarine sediments and native Olympia oysters: A contrast between a developed and an undeveloped estuary

USGS Publications Warehouse

Granek, Elise F.; Conn, Kathleen E.; Nilsen, Elena B.; Pillsbury, Lori; Strecker, Angela L.; Rumrill, Steve; Fish, William

2016-01-01

Chemical contaminants can be introduced into estuarine and marine ecosystems from a variety of sources including wastewater, agriculture and forestry practices, point and non-point discharges, runoff from industrial, municipal, and urban lands, accidental spills, and atmospheric deposition. The diversity of potential sources contributes to the likelihood of contaminated marine waters and sediments and increases the probability of uptake by marine organisms. Despite widespread recognition of direct and indirect pathways for contaminant deposition and organismal exposure in coastal systems, spatial and temporal variability in contaminant composition, deposition, and uptake patterns are still poorly known. We investigated these patterns for a suite of persistent legacy contaminants including polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) and chemicals of emerging concern including pharmaceuticals within two Oregon coastal estuaries (Coos and Netarts Bays). In the more urbanized Coos Bay, native Olympia oyster (Ostrea lurida) tissue had approximately twice the number of PCB congeners at over seven times the total concentration, yet fewer PBDEs at one-tenth the concentration as compared to the more rural Netarts Bay. Different pharmaceutical suites were detected during each sampling season. Variability in contaminant types and concentrations across seasons and between species and media (organisms versus sediment) indicates the limitation of using indicator species and/or sampling annually to determine contaminant loads at a site or for specific species. The results indicate the prevalence of legacy contaminants and CECs in relatively undeveloped coastal environments highlighting the need to improve policy and management actions to reduce contaminant releases into estuarine and marine waters and to deal with legacy compounds that remain long after prohibition of use. Our results point to the need for better understanding of the ecological and
Recent Intermediate Depth Earthquakes in El Salvador, Central Mexico, Cascadia and South-West Japan

NASA Astrophysics Data System (ADS)

Lemoine, A.; Gardi, A.; Gutscher, M.; Madariaga, R.

2001-12-01

We studied occurence and source parameters of several recent intermediate depth earthquakes. We concentrated on the Mw=7.7 salvadorian earthquake which took place on January 13, 2001. It was a good example of the high seismic risk associated to such kind of events which occur closer to the coast than the interplate thrust events. The Salvadorian earthquake was an intermediate depth downdip extensional event which occured inside the downgoing Cocos plate, next to the downdip flexure where the dip increases sharply before the slab sinks more steeply. This location corresponds closely to the position of the Mw=5.7 1996 and Mw=7.3 1982 downdip extensional events. Several recent intermediate depth earthquakes occured in subduction zones exhibiting a ``flat slab'' geometry with three distinct flexural bends where flexural stress may be enhanced. The Mw=6.7 Geiyo event showed a downdip extensional mechanism with N-S striking nodal planes. This trend was highly oblique to the trench (Nankai Trough), yet consistent with westward steepening at the SW lateral termination of the SW Japan flat slab. The Mw=6.8 Olympia earthquake in the Cascadia subduction zone occured at the downdip termination of the Juan de Fuca slab, where plate dip increases from about 5o to over 30o. The N-S orientation of the focal planes, parallel to the trench indicated downdip extension. The location at the downdip flexure corresponds closely to the estimated positions of the 1949 M7.1 Olympia and 1965 M6.5 Seattle-Tacoma events. Between 1994 and 1999, in Central Mexico, an unusually high intermediate depth seismicity occured where several authors proposed a flat geometry for the Cocos plate. Seven events of magnitude between Mw=5.9 and Mw=7.1 occured. Three of them were downdip compressional and four where down-dip extensional. We can explain these earthquakes by flexural stresses at down-dip and lateral terminations of the supposed flat segment. Even if intermediate depth earthquakes occurence could
45 CFR 162.1402 - Standards for health care claim status transaction.

Code of Federal Regulations, 2012 CFR

2012-10-01

... Electronic Data Interchange Technical Report Type 3, April 2008, ASC X12N/005010X212E1. (Incorporated by... paragraph (a) of this section; and (2) The ASC X12 Standards for Electronic Data Interchange Technical Report Type 3—Health Care Claim Status Request and Response (276/277), August 2006, ASC X12N/005010X212...
45 CFR 162.1402 - Standards for health care claim status transaction.

Code of Federal Regulations, 2013 CFR

2013-10-01

... Electronic Data Interchange Technical Report Type 3, April 2008, ASC X12N/005010X212E1. (Incorporated by... paragraph (a) of this section; and (2) The ASC X12 Standards for Electronic Data Interchange Technical Report Type 3—Health Care Claim Status Request and Response (276/277), August 2006, ASC X12N/005010X212...
45 CFR 162.1402 - Standards for health care claim status transaction.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Electronic Data Interchange Technical Report Type 3, April 2008, ASC X12N/005010X212E1. (Incorporated by... paragraph (a) of this section; and (2) The ASC X12 Standards for Electronic Data Interchange Technical Report Type 3—Health Care Claim Status Request and Response (276/277), August 2006, ASC X12N/005010X212...
45 CFR 162.1402 - Standards for health care claim status transaction.

Code of Federal Regulations, 2014 CFR

2014-10-01

... Electronic Data Interchange Technical Report Type 3, April 2008, ASC X12N/005010X212E1. (Incorporated by... paragraph (a) of this section; and (2) The ASC X12 Standards for Electronic Data Interchange Technical Report Type 3—Health Care Claim Status Request and Response (276/277), August 2006, ASC X12N/005010X212...
Hydro pumped storage, international experience: An overview of ASCE task committee report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Makarechian, A.H.; Rummel, G.

1995-12-31

This paper presents an overview of a report that is being prepared by ASCE Task Committee on Pumped Storage, International Experience. The reader is referred to the committee report that will be available in 1996. Many pumped storage projects in Europe, but particularly in Japan are becoming an indispensable resource in management of loads and resources on the electrical system. They serve to enhance reliability of the system and to provide for efficient utilization of thermal resources. Pumped storage is increasingly being used as a system management tool. To serve such purposes and to function in this key role, pumpedmore » storage projects are designed for very fast loading and unloading, for very fast mode reversals from pumping to generating and visa versa, for synchronous generation, and more importantly for load ramping during the pumping mode. This is achieved by use of variable-speed pump turbine units. The use of variable-speed units has proven so successful in Japan that many older projects are retrofitted with this new feature. Other interesting equipment applications are discussed including utilization of multi-stage unregulated pump turbines for very high heads (up to 1,250 m), and continued extension of the experience for high head reversible Francis unit, currently in excess of 750 m.« less
45 CFR 162.1402 - Standards for health care claim status transaction.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Electronic Data Interchange Technical Report Type 3, April 2008, ASC X12N/005010X212E1. (Incorporated by.... 162.1402 Section 162.1402 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES ADMINISTRATIVE DATA... paragraph (a) of this section; and (2) The ASC X12 Standards for Electronic Data Interchange Technical...
View of a five inch standard Mark III model 1 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View of a five inch standard Mark III model 1 #39, manufactured in 1916 at the naval gun factory waterveliet, NY; this is the only gun remaining on olympia dating from the period when it was in commission; note ammunition lift at left side of photograph. (p36) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
Small Business Contracting in the United States and Europe: A Comparative Assessment

DTIC Science & Technology

2010-03-01

Counsel for procurement, innovation, and oversight matters at the US Senate Committee on Small Business & Entrepreneurship , then chaired by Senator Olympia... Entrepreneurship , then chaired by Senator Olympia J. Snowe (R-ME), the author‘s responsibilities included oversight of Federal small business...authorities on both sides of the Atlantic use the demand created by government contracts to stimulate competitive private entrepreneurship and greater
The Cladophora glomerata Enriched by Biosorption Process in Cr(III) Improves Viability, and Reduces Oxidative Stress and Apoptosis in Equine Metabolic Syndrome Derived Adipose Mesenchymal Stromal Stem Cells (ASCs) and Their Extracellular Vesicles (MV's).

PubMed

Marycz, Krzysztof; Michalak, Izabela; Kocherova, Ievgeniia; Marędziak, Monika; Weiss, Christine

2017-12-08

This study investigated in vitro effects of freshwater alga Cladophora glomerata water extract enriched during a biosorption process in Cr(III) trivalent chromium and chromium picolinate on adipose-derived mesenchymal stromal stem cells (ASCs) and extracellular microvesicles (MVs) in equine metabolic syndrome-affected horses. Chemical characterisation of natural Cladophora glomerata was performed with special emphasis on: vitamin C, vitamin E, total phenols, fatty acids, free and protein-bound amino acids as well as measured Cr in algal biomass. To examine the influence of Cladophora glomerata water extracts, in vitro viability, oxidative stress factor accumulation, apoptosis, inflammatory response, biogenesis of mitochondria, autophagy in ASCs of EMS and secretory activity manifested by MV release were investigated. For this purpose, various methods of molecular biology and microscopic observations (i.e., immunofluorescence staining, SEM, TEM, FIB observations, mRNA and microRNA expression by RT-qPCR) were applied. The extract of Cladophora glomerata enriched with Cr(III) ions reduced apoptosis and inflammation in ASCs of EMS horses through improvement of mitochondrial dynamics, decreasing of PDK4 expression and reduction of endoplastic reticulum stress. Moreover, it was found, that Cladophora glomerata and Cr(III) induce antioxidative protection coming from enhanced SOD activity Therefore, Cladophora glomerata enriched with Cr(III) ions might become an interesting future therapeutic agent in the pharmacological treatment of EMS horses.
Profile formation of academic self-concept in elementary school students in grades 1 to 4.

PubMed

Schmidt, Isabelle; Brunner, Martin; Keller, Lena; Scherrer, Vsevolod; Wollschläger, Rachel; Baudson, Tanja Gabriele; Preckel, Franzis

2017-01-01

Academic self-concept (ASC) is comprised of individual perceptions of one's own academic ability. In a cross-sectional quasi-representative sample of 3,779 German elementary school children in grades 1 to 4, we investigated (a) the structure of ASC, (b) ASC profile formation, an aspect of differentiation that is reflected in lower correlations between domain-specific ASCs with increasing grade level, (c) the impact of (internal) dimensional comparisons of one's own ability in different school subjects for profile formation of ASC, and (d) the role played by differences in school grades between subjects for these dimensional comparisons. The nested Marsh/Shavelson model, with general ASC at the apex and math, writing, and reading ASC as specific factors nested under general ASC fitted the data at all grade levels. A first-order factor model with math, writing, reading, and general ASCs as correlated factors provided a good fit, too. ASC profile formation became apparent during the first two to three years of school. Dimensional comparisons across subjects contributed to ASC profile formation. School grades enhanced these comparisons, especially when achievement profiles were uneven. In part, findings depended on the assumed structural model of ASCs. Implications for further research are discussed with special regard to factors influencing and moderating dimensional comparisons.

Profile formation of academic self-concept in elementary school students in grades 1 to 4

PubMed Central

Schmidt, Isabelle; Brunner, Martin; Keller, Lena; Scherrer, Vsevolod; Wollschläger, Rachel; Baudson, Tanja Gabriele; Preckel, Franzis

2017-01-01

Academic self-concept (ASC) is comprised of individual perceptions of one’s own academic ability. In a cross-sectional quasi-representative sample of 3,779 German elementary school children in grades 1 to 4, we investigated (a) the structure of ASC, (b) ASC profile formation, an aspect of differentiation that is reflected in lower correlations between domain-specific ASCs with increasing grade level, (c) the impact of (internal) dimensional comparisons of one’s own ability in different school subjects for profile formation of ASC, and (d) the role played by differences in school grades between subjects for these dimensional comparisons. The nested Marsh/Shavelson model, with general ASC at the apex and math, writing, and reading ASC as specific factors nested under general ASC fitted the data at all grade levels. A first-order factor model with math, writing, reading, and general ASCs as correlated factors provided a good fit, too. ASC profile formation became apparent during the first two to three years of school. Dimensional comparisons across subjects contributed to ASC profile formation. School grades enhanced these comparisons, especially when achievement profiles were uneven. In part, findings depended on the assumed structural model of ASCs. Implications for further research are discussed with special regard to factors influencing and moderating dimensional comparisons. PMID:28542384
Atypical Squamous Cells in Liquid-Based Cervical Cytology: Microbiology, Inflammatory Infiltrate, and Human Papillomavirus-DNA Testing.

PubMed

Gomes de Oliveira, Geilson; Eleutério, Renata Mirian Nunes; Silveira Gonçalves, Ana Katherine; Giraldo, Paulo César; Eleutério, José

2018-01-01

The aim of this study was to assess the correlation between atypical squamous cells (ASC) and inflammatory infiltrate and vaginal microbiota using cervical liquid-based cytological (SurePath®) and high-risk human papillomavirus (HR-HPV) tests. A cross-sectional study was conducted using a 6-year database from a laboratory in Fortaleza (Brazil). Files from 1,346 ASC cases were divided into subgroups and results concerning inflammation and vaginal microorganisms diagnosed by cytology were compared with HR-HPV test results. An absence of specific microorganisms (ASM) was the most frequent finding (ASC of undetermined significance, ASC-US = 74%; ASC - cannot exclude high-grade squamous intraepithelial lesion, ASC-H = 68%), followed by bacterial vaginosis (ASC-US = 20%; ASC- H = 25%) and Candida spp. (ASC-US = 6%; ASC-H = 5%). Leukocyte infiltrate was present in 71% of ASC-US and 85% of ASC-H (p = 0.0040), and in these specific cases HR-HPV tests were positive for 65 and 64%, respectively. A positive HR-HPV test was relatively more frequent when a specific microorganism was present, and Candida spp. was associated with HR-HPV-positive results (p = 0.0156), while an ASM was associated with negative HR-HPV results (p = 0.0370). ASC-US is associated with an absence of inflammation or vaginosis, while ASC-H smears are associated with Trichomonas vaginalis and inflammatory infiltrate. A positive HR-HPV is associated with Candida spp. in ASC cytology. © 2017 S. Karger AG, Basel.
ATP Induces IL-1β Secretion in Neisseria gonorrhoeae-Infected Human Macrophages by a Mechanism Not Related to the NLRP3/ASC/Caspase-1 Axis

PubMed Central

García, Killen; Escobar, Gisselle; Mendoza, Pablo; Beltran, Caroll; Perez, Claudio; Vernal, Rolando; Acuña-Castillo, Claudio

2016-01-01

Neisseria gonorrhoeae (Ngo) has developed multiple immune evasion mechanisms involving the innate and adaptive immune responses. Recent findings have reported that Ngo reduces the IL-1β secretion of infected human monocyte-derived macrophages (MDM). Here, we investigate the role of adenosine triphosphate (ATP) in production and release of IL-1β in Ngo-infected MDM. We found that the exposure of Ngo-infected MDM to ATP increases IL-1β levels about ten times compared with unexposed Ngo-infected MDM (P < 0.01). However, we did not observe any changes in inflammasome transcriptional activation of speck-like protein containing a caspase recruitment domain (CARD) (ASC, P > 0.05) and caspase-1 (CASP1, P > 0.05). In addition, ATP was not able to modify caspase-1 activity in Ngo-infected MDM but was able to increase pyroptosis (P > 0.01). Notably ATP treatment defined an increase of positive staining for IL-1β with a distinctive intracellular pattern of distribution. Collectively, these data demonstrate that ATP induces IL-1β secretion by a mechanism not related to the NLRP3/ASC/caspase-1 axis and likely is acting at the level of vesicle trafficking or pore formation. PMID:27803513
75 FR 73059 - Record of Decision and Floodplain Statement of Findings for the Cushman Hydroelectric Project...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-29

... license to include inter alia conditions for fish passage facilities and authorization to construct the... originally opposed Tacoma's Cultural Resource Summary Report, and cultural resource protection became a... in the MOA. The MOA includes a Treatment Plan that Tacoma must follow to protect cultural resources...
The Cladophora glomerata Enriched by Biosorption Process in Cr(III) Improves Viability, and Reduces Oxidative Stress and Apoptosis in Equine Metabolic Syndrome Derived Adipose Mesenchymal Stromal Stem Cells (ASCs) and Their Extracellular Vesicles (MV’s)

PubMed Central

Marycz, Krzysztof; Marędziak, Monika; Weiss, Christine

2017-01-01

This study investigated in vitro effects of freshwater alga Cladophora glomerata water extract enriched during a biosorption process in Cr(III) trivalent chromium and chromium picolinate on adipose-derived mesenchymal stromal stem cells (ASCs) and extracellular microvesicles (MVs) in equine metabolic syndrome-affected horses. Chemical characterisation of natural Cladophora glomerata was performed with special emphasis on: vitamin C, vitamin E, total phenols, fatty acids, free and protein-bound amino acids as well as measured Cr in algal biomass. To examine the influence of Cladophora glomerata water extracts, in vitro viability, oxidative stress factor accumulation, apoptosis, inflammatory response, biogenesis of mitochondria, autophagy in ASCs of EMS and secretory activity manifested by MV release were investigated. For this purpose, various methods of molecular biology and microscopic observations (i.e., immunofluorescence staining, SEM, TEM, FIB observations, mRNA and microRNA expression by RT-qPCR) were applied. The extract of Cladophora glomerata enriched with Cr(III) ions reduced apoptosis and inflammation in ASCs of EMS horses through improvement of mitochondrial dynamics, decreasing of PDK4 expression and reduction of endoplastic reticulum stress. Moreover, it was found, that Cladophora glomerata and Cr(III) induce antioxidative protection coming from enhanced SOD activity Therefore, Cladophora glomerata enriched with Cr(III) ions might become an interesting future therapeutic agent in the pharmacological treatment of EMS horses. PMID:29292726
Inflammasome components ASC and AIM2 modulate the acute phase of biomaterial implant-induced foreign body responses

PubMed Central

Christo, Susan N.; Diener, Kerrilyn R.; Manavis, Jim; Grimbaldeston, Michele A.; Bachhuka, Akash; Vasilev, Krasimir; Hayball, John D.

2016-01-01

Detailing the inflammatory mechanisms of biomaterial-implant induced foreign body responses (FBR) has implications for revealing targetable pathways that may reduce leukocyte activation and fibrotic encapsulation of the implant. We have adapted a model of poly(methylmethacrylate) (PMMA) bead injection to perform an assessment of the mechanistic role of the ASC-dependent inflammasome in this process. We first demonstrate that ASC−/− mice subjected to PMMA bead injections had reduced cell infiltration and altered collagen deposition, suggesting a role for the inflammasome in the FBR. We next investigated the NLRP3 and AIM2 sensors because of their known contributions in recognising damaged and apoptotic cells. We found that NLRP3 was dispensable for the fibrotic encapsulation; however AIM2 expression influenced leukocyte infiltration and controlled collagen deposition, suggesting a previously unexplored link between AIM2 and biomaterial-induced FBR. PMID:26860464
Contract W911NF-09-1-0105 (Monterey Institute of International Studies)

DTIC Science & Technology

2014-04-08

the International Association for Conflict Management , in Tacoma, Washington, June 30, 2013-July 3, 2013. (This version of the paper was provided to...Extraordinary Measure or Business as Usual?” at the 26th Annual Conference of the International Association for Conflict Management , in Tacoma, Washington
33 CFR 165.1329 - Regulated Navigation Area; Thea Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site...

Code of Federal Regulations, 2014 CFR

2014-07-01

... Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site, Commencement Bay, Tacoma, WA. 165.1329... Area; Thea Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site, Commencement Bay, Tacoma, WA... (EPA) and others in the Thea Foss and Wheeler-Osgood Waterways EPA superfund cleanup site. Vessels may...
33 CFR 165.1329 - Regulated Navigation Area; Thea Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site...

Code of Federal Regulations, 2011 CFR

2011-07-01

... Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site, Commencement Bay, Tacoma, WA. 165.1329... Area; Thea Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site, Commencement Bay, Tacoma, WA... (EPA) and others in the Thea Foss and Wheeler-Osgood Waterways EPA superfund cleanup site. Vessels may...
33 CFR 165.1329 - Regulated Navigation Area; Thea Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site...

Code of Federal Regulations, 2013 CFR

2013-07-01

... Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site, Commencement Bay, Tacoma, WA. 165.1329... Area; Thea Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site, Commencement Bay, Tacoma, WA... (EPA) and others in the Thea Foss and Wheeler-Osgood Waterways EPA superfund cleanup site. Vessels may...
33 CFR 165.1329 - Regulated Navigation Area; Thea Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site...

Code of Federal Regulations, 2012 CFR

2012-07-01

... Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site, Commencement Bay, Tacoma, WA. 165.1329... Area; Thea Foss and Wheeler-Osgood Waterways EPA Superfund Cleanup Site, Commencement Bay, Tacoma, WA... (EPA) and others in the Thea Foss and Wheeler-Osgood Waterways EPA superfund cleanup site. Vessels may...
Certification of Completion of ASC FY08 Level-2 Milestone ID #2933

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lipari, D A

2008-06-12

This report documents the satisfaction of the completion criteria associated with ASC FY08 Milestone ID No.2933: 'Deploy Moab resource management services on BlueGene/L'. Specifically, this milestone represents LLNL efforts to enhance both SLURM and Moab to extend Moab's capabilities to schedule and manage BlueGene/L, and increases portability of user scripts between ASC systems. The completion criteria for the milestone are the following: (1) Batch jobs can be specified, submitted to Moab, scheduled and run on the BlueGene/L system; (2) Moab will be able to support the markedly increased scale in node count as well as the wiring geometry that ismore » unique to BlueGene/L; and (3) Moab will also prepare and report statistics of job CPU usage just as it does for the current systems it supports. This document presents the completion evidence for both of the stated milestone certification methods: Completion evidence for this milestone will be in the form of (1) documentation--a report that certifies that the completion criteria have been met; and (2) user hand-off. As the selected Tri-Lab workload manager, Moab was chosen to replace LCRM as the enterprise-wide scheduler across Livermore Computing (LC) systems. While LCRM/SLURM successfully scheduled jobs on BG/L, the effort to replace LCRM with Moab on BG/L represented a significant challenge. Moab is a commercial product developed and sold by Cluster Resources, Inc. (CRI). Moab receives the users batch job requests and dispatches these jobs to run on a specific cluster. SLURM is an open-source resource manager whose development is managed by members of the Integrated Computational Resource Management Group (ICRMG) within the Services and Development Division at LLNL. SLURM is responsible for launching and running jobs on an individual cluster. Replacing LCRM with Moab on BG/L required substantial changes to both Moab and SLURM. While the ICRMG could directly manage the SLURM development effort, the work to
The western limits of the Seattle fault zone and its interaction with the Olympic Peninsula, Washington

USGS Publications Warehouse

A.P. Lamb,; L.M. Liberty,; Blakely, Richard J.; Pratt, Thomas L.; Sherrod, B.L.; Van Wijk, K.

2012-01-01

We present evidence that the Seattle fault zone of Washington State extends to the west edge of the Puget Lowland and is kinemati-cally linked to active faults that border the Olympic Massif, including the Saddle Moun-tain deformation zone. Newly acquired high-resolution seismic reflection and marine magnetic data suggest that the Seattle fault zone extends west beyond the Seattle Basin to form a >100-km-long active fault zone. We provide evidence for a strain transfer zone, expressed as a broad set of faults and folds connecting the Seattle and Saddle Mountain deformation zones near Hood Canal. This connection provides an explanation for the apparent synchroneity of M7 earthquakes on the two fault systems ~1100 yr ago. We redeﬁ ne the boundary of the Tacoma Basin to include the previously termed Dewatto basin and show that the Tacoma fault, the southern part of which is a backthrust of the Seattle fault zone, links with a previously unidentiﬁ ed fault along the western margin of the Seattle uplift. We model this north-south fault, termed the Dewatto fault, along the western margin of the Seattle uplift as a low-angle thrust that initiated with exhu-mation of the Olympic Massif and today accommodates north-directed motion. The Tacoma and Dewatto faults likely control both the southern and western boundaries of the Seattle uplift. The inferred strain trans-fer zone linking the Seattle fault zone and Saddle Mountain deformation zone deﬁ nes the northern margin of the Tacoma Basin, and the Saddle Mountain deformation zone forms the northwestern boundary of the Tacoma Basin. Our observations and model suggest that the western portions of the Seattle fault zone and Tacoma fault are com-plex, require temporal variations in principal strain directions, and cannot be modeled as a simple thrust and/or backthrust system.
A report documenting the completion of the Los Alamos National Laboratory portion of the ASC level II milestone ""Visualization on the supercomputing platform

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ahrens, James P; Patchett, John M; Lo, Li - Ta

2011-01-24

This report provides documentation for the completion of the Los Alamos portion of the ASC Level II 'Visualization on the Supercomputing Platform' milestone. This ASC Level II milestone is a joint milestone between Sandia National Laboratory and Los Alamos National Laboratory. The milestone text is shown in Figure 1 with the Los Alamos portions highlighted in boldfaced text. Visualization and analysis of petascale data is limited by several factors which must be addressed as ACES delivers the Cielo platform. Two primary difficulties are: (1) Performance of interactive rendering, which is the most computationally intensive portion of the visualization process. Formore » terascale platforms, commodity clusters with graphics processors (GPUs) have been used for interactive rendering. For petascale platforms, visualization and rendering may be able to run efficiently on the supercomputer platform itself. (2) I/O bandwidth, which limits how much information can be written to disk. If we simply analyze the sparse information that is saved to disk we miss the opportunity to analyze the rich information produced every timestep by the simulation. For the first issue, we are pursuing in-situ analysis, in which simulations are coupled directly with analysis libraries at runtime. This milestone will evaluate the visualization and rendering performance of current and next generation supercomputers in contrast to GPU-based visualization clusters, and evaluate the perfromance of common analysis libraries coupled with the simulation that analyze and write data to disk during a running simulation. This milestone will explore, evaluate and advance the maturity level of these technologies and their applicability to problems of interest to the ASC program. In conclusion, we improved CPU-based rendering performance by a a factor of 2-10 times on our tests. In addition, we evaluated CPU and CPU-based rendering performance. We encourage production visualization experts to consider
Development of Home Health Aide Curriculum Project. Final Report.

ERIC Educational Resources Information Center

Brown, Patricia

This package contains materials intended for use in a new home health aide curriculum that is designed to be presented as a two-quarter program at Tacoma Community College in Tacoma, Washington. Included in the package are a final report outlining the objectives and outcomes of the project to develop a home health aide curriculum that would meet…
Endocytosis of indium-tin-oxide nanoparticles by macrophages provokes pyroptosis requiring NLRP3-ASC-Caspase1 axis that can be prevented by mesenchymal stem cells

PubMed Central

Naji, Abderrahim; Muzembo, Basilua André; Yagyu, Ken-ichi; Baba, Nobuyasu; Deschaseaux, Frédéric; Sensebé, Luc; Suganuma, Narufumi

2016-01-01

The biological effects of indium-tin-oxide (ITO) are of considerable importance because workers exposed to indium compounds have been diagnosed with interstitial lung disease or pulmonary alveolar proteinosis; however, the pathophysiology of these diseases is undefined. Here, mice intraperitoneally inoculated with ITO-nanoparticles (ITO-NPs) resulted in peritonitis dependent in NLRP3 inflammasome, with neutrophils recruitment and interleukin-1β (IL-1β) production. Withal peritoneal macrophages exposed ex vivo to ITO-NPs caused IL-1β secretion and cytolysis. Further, alveolar macrophages exposed to ITO-NPs in vitro showed ITO-NP endocytosis and production of tumor necrosis factor-α (TNF-α) and IL-1β, ensued cell death by cytolysis. This cell death was RIPK1-independent but caspase1-dependent, and thus identified as pyroptosis. Endocytosis of ITO-NPs by activated THP-1 cells induced pyroptosis with IL-1β/TNF-α production and cytolysis, but not in activated THP-1 cells with knockdown of NLRP3, ASC, or caspase1. However, exposing activated THP-1 cells with NLRP3 or ASC knockdown to ITO-NPs resulted in cell death but without cytolysis, with deficiency in IL-1β/TNF-α, and revealing features of apoptosis. While, mesenchymal stem cells (MSCs) co-cultured with macrophages impaired both inflammation and cell death induced by ITO-NPs. Together, our findings provide crucial insights to the pathophysiology of respiratory diseases caused by ITO particles, and identify MSCs as a potent therapeutic. PMID:27194621
42 CFR 416.41 - Condition for coverage-Governing body and management.

Code of Federal Regulations, 2010 CFR

2010-10-01

... governing the ASC's total operation. The governing body has oversight and accountability for the quality... management. 416.41 Section 416.41 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF... for Coverage § 416.41 Condition for coverage—Governing body and management. The ASC must have a...
42 CFR 416.41 - Condition for coverage-Governing body and management.

Code of Federal Regulations, 2011 CFR

2011-10-01

... governing the ASC's total operation. The governing body has oversight and accountability for the quality... management. 416.41 Section 416.41 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF... for Coverage § 416.41 Condition for coverage—Governing body and management. The ASC must have a...
42 CFR 416.167 - Basis of payment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... classification (APC) groups and payment weights. (1) ASC covered surgical procedures are classified using the APC... section, an ASC relative payment weight is determined based on the APC relative payment weight for each covered surgical procedure and covered ancillary service that has an applicable APC relative payment...
HPV genotype profile in a Norwegian cohort with ASC-US and LSIL cytology with three year cumulative risk of high grade cervical neoplasia.

PubMed

Lie, A K; Tropé, A; Skare, G B; Bjørge, T; Jonassen, C M; Brusegard, K; Lönnberg, S

2018-01-01

To explore the HPVgenotype profile in Norwegian women with ASC-US/LSIL cytology and the subsequent risk of high-grade cervical neoplasia (CIN 3+). In this observational study delayed triage of ASC-US/LSIL of 6058 women were included from 2005 to 2010. High-risk HPV detection with Hybrid Capture 2 (HC2) was used and the HC2+ cases were genotyped with in-house nmPCR. Women were followed-up for histologically confirmed CIN3+ within three years of index HPV test by linkage to the screening databases at the Cancer Registry of Norway. HC2 was positive in 45% (2756/6058) of the women. Within 3years CIN3+ was diagnosed in 26% of women<34year and in 15%≥34year. HC2 was positive at index in 94% of CIN3+ cases and negative in 64 cases including three women with cervical carcinomas. Women<34years with single infections of HPV 16, 35, 58 or 33 or multiple infections including HPV 16, 52, 33 or 31 were associated with highest proportions of CIN 3+. Older women with single infection with HPV 16, 33, 31 or 35 or multiple infections including HPV 16, 33, 31 or 18/39 were more likely to develop CIN 3+. HPV 16 and HPV 33 at baseline both as single or multiple infections, were associated with the highest risk for CIN3+. Among older women, all 13 high-risk genotypes as single infection were associated with >20% risk of CIN3+. Further studies are necessary to risk stratify the individual genotypes to reduce the number of colposcopies in Norway. Copyright © 2017 Elsevier Inc. All rights reserved.

ASCS online fault detection and isolation based on an improved MPCA

NASA Astrophysics Data System (ADS)

Peng, Jianxin; Liu, Haiou; Hu, Yuhui; Xi, Junqiang; Chen, Huiyan

2014-09-01

Multi-way principal component analysis (MPCA) has received considerable attention and been widely used in process monitoring. A traditional MPCA algorithm unfolds multiple batches of historical data into a two-dimensional matrix and cut the matrix along the time axis to form subspaces. However, low efficiency of subspaces and difficult fault isolation are the common disadvantages for the principal component model. This paper presents a new subspace construction method based on kernel density estimation function that can effectively reduce the storage amount of the subspace information. The MPCA model and the knowledge base are built based on the new subspace. Then, fault detection and isolation with the squared prediction error (SPE) statistic and the Hotelling ( T 2) statistic are also realized in process monitoring. When a fault occurs, fault isolation based on the SPE statistic is achieved by residual contribution analysis of different variables. For fault isolation of subspace based on the T 2 statistic, the relationship between the statistic indicator and state variables is constructed, and the constraint conditions are presented to check the validity of fault isolation. Then, to improve the robustness of fault isolation to unexpected disturbances, the statistic method is adopted to set the relation between single subspace and multiple subspaces to increase the corrective rate of fault isolation. Finally fault detection and isolation based on the improved MPCA is used to monitor the automatic shift control system (ASCS) to prove the correctness and effectiveness of the algorithm. The research proposes a new subspace construction method to reduce the required storage capacity and to prove the robustness of the principal component model, and sets the relationship between the state variables and fault detection indicators for fault isolation.
Characterisation and cloning of a Na(+)-dependent broad-specificity neutral amino acid transporter from NBL-1 cells: a novel member of the ASC/B(0) transporter family.

PubMed

Pollard, Matthew; Meredith, David; McGivan, John D

2002-04-12

Na(+)-dependent neutral amino acid transport into the bovine renal epithelial cell line NBL-1 is catalysed by a broad-specificity transporter originally termed System B(0). This transporter is shown to differ in specificity from the B(0) transporter cloned from JAR cells [J. Biol. Chem. 271 (1996) 18657] in that it interacts much more strongly with phenylalanine. Using probes designed to conserved transmembrane regions of the ASC/B(0) transporter family we have isolated a cDNA encoding the NBL-1 cell System B(0) transporter. When expressed in Xenopus oocytes the clone catalysed Na(+)-dependent alanine uptake which was inhibited by glutamine, leucine and phenylalanine. However, the clone did not catalyse Na(+)-dependent phenylalanine transport, again as in NBL-1 cells. The clone encoded a protein of 539 amino acids; the predicted transmembrane domains were almost identical in sequence to those of the other members of the B(0)/ASC transporter family. Comparison of the sequences of NBL-1 and JAR cell transporters showed some differences near the N-terminus, C-terminus and in the loop between helices 3 and 4. The NBL-1 B(0) transporter is not the same as the renal brush border membrane transporter since it does not transport phenylalanine. Differences in specificity in this protein family arise from relatively small differences in amino acid sequence.
42 CFR 416.44 - Condition for coverage-Environment.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 3 2013-10-01 2013-10-01 false Condition for coverage-Environment. 416.44 Section....44 Condition for coverage—Environment. The ASC must have a safe and sanitary environment, properly... environment. The ASC must provide a functional and sanitary environment for the provision of surgical services...
Commencement Bay Study. Volume VII. Sediments, Noise, Climate, and Air Quality, Aesthetics, Birds.

DTIC Science & Technology

1981-12-31

Island (Carpenter et al. 1978). While the arsenic found in sediments near the smelter is almost entirely contained in slag (bulk and particles) and is...indicates slag in a freshwater, low pH environment does leach both arsenic and other selected heavy metals. See the EIS documents for the proposed ASARCO...flood protection to the industrial section of Tacoma through the channelization of the river, and the construction of levees and revetments (U.S. Army
Summary Report for ASC L2 Milestone #4782: Assess Newly Emerging Programming and Memory Models for Advanced Architectures on Integrated Codes

DOE Office of Scientific and Technical Information (OSTI.GOV)

Neely, J. R.; Hornung, R.; Black, A.

This document serves as a detailed companion to the powerpoint slides presented as part of the ASC L2 milestone review for Integrated Codes milestone #4782 titled “Assess Newly Emerging Programming and Memory Models for Advanced Architectures on Integrated Codes”, due on 9/30/2014, and presented for formal program review on 9/12/2014. The program review committee is represented by Mike Zika (A Program Project Lead for Kull), Brian Pudliner (B Program Project Lead for Ares), Scott Futral (DEG Group Lead in LC), and Mike Glass (Sierra Project Lead at Sandia). This document, along with the presentation materials, and a letter of completionmore » signed by the review committee will act as proof of completion for this milestone.« less
Cervical cytology of atypical squamous cells, cannot exclude high-grade squamous intra-epithelial lesion: significance of age, human papillomavirus DNA detection and previous abnormal cytology on follow-up outcomes.

PubMed

Sung, Chang Ohk; Oh, Young Lyun; Song, Sang Yong

2011-11-01

Despite the usefulness of Pap tests for cancer screening, outcomes can be difficult to predict when atypical squamous cells (ASCs) are identified. According to the 2001 Bethesda system, ASCs can be subdivided into two groups: ASCs of undetermined significance (ASC-US); and ASCs, cannot exclude high-grade squamous intra-epithelial lesion (ASC-H). ASC-H interpretations are uncommon, and studies involving this type of lesion are based on small numbers of cases. Cross-sectional, retrospective study of 392 ASC-H cases. The follow-up outcomes of ASC-H cases that were diagnosed during routine primary screening between 2002 and 2008 were investigated, and relationships between clinicopathological parameters were assessed, particularly positive test for high-risk HPV (HPV) DNA, patient age at diagnosis and previous abnormal cytology. Of the 392 cases, high-grade squamous intra-epithelial lesion (HSIL) was detected in 111 (28.3%) cases, squamous cell carcinoma was detected in 15 (3.8%) cases, low-grade squamous intra-epithelial lesion was detected in 37 (9.4%) cases, reactive change was detected in 178 (45.4%) cases, atrophy was detected in 47 (12.0%) cases, and adenocarcinoma was detected in four (1.0%) cases. The prevalence of HSIL or greater was 27.8% for women aged ≥ 40 years, and 52.3% for women aged <40 years (p<0.001). HPV positivity in ASC-H smears was significantly associated with HSIL or greater, irrespective of age (<40 years, p=0.003; ≥ 40 years, p<0.001). ASC-H with previous abnormal cytology greater than ASC-US showed a significantly higher detection rate for HSIL or greater at follow-up (p<0.001). Patient age, positive HPV DNA test and previous abnormal cytology are useful predictors of underlying HSIL or greater in women with ASC-H. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Engineering anatomically shaped vascularized bone grafts with hASCs and 3D-printed PCL scaffolds.

PubMed

Temple, Joshua P; Hutton, Daphne L; Hung, Ben P; Huri, Pinar Yilgor; Cook, Colin A; Kondragunta, Renu; Jia, Xiaofeng; Grayson, Warren L

2014-12-01

The treatment of large craniomaxillofacial bone defects is clinically challenging due to the limited availability of transplantable autologous bone grafts and the complex geometry of the bones. The ability to regenerate new bone tissues that faithfully replicate the anatomy would revolutionize treatment options. Advances in the field of bone tissue engineering over the past few decades offer promising new treatment alternatives using biocompatible scaffold materials and autologous cells. This approach combined with recent advances in three-dimensional (3D) printing technologies may soon allow the generation of large, bioartificial bone grafts with custom, patient-specific architecture. In this study, we use a custom-built 3D printer to develop anatomically shaped polycaprolactone (PCL) scaffolds with varying internal porosities. These scaffolds are assessed for their ability to support induction of human adipose-derived stem cells (hASCs) to form vasculature and bone, two essential components of functional bone tissue. The development of functional tissues is assessed in vitro and in vivo. Finally, we demonstrate the ability to print large mandibular and maxillary bone scaffolds that replicate fine details extracted from patient's computed tomography scans. The findings of this study illustrate the capabilities and potential of 3D printed scaffolds to be used for engineering autologous, anatomically shaped, vascularized bone grafts. © 2014 Wiley Periodicals, Inc.
High-resolution global grids of revised Priestley-Taylor and Hargreaves-Samani coefficients for assessing ASCE-standardized reference crop evapotranspiration and solar radiation

NASA Astrophysics Data System (ADS)

Aschonitis, Vassilis G.; Papamichail, Dimitris; Demertzi, Kleoniki; Colombani, Nicolo; Mastrocicco, Micol; Ghirardini, Andrea; Castaldelli, Giuseppe; Fano, Elisa-Anna

2017-08-01

The objective of the study is to provide global grids (0.5°) of revised annual coefficients for the Priestley-Taylor (P-T) and Hargreaves-Samani (H-S) evapotranspiration methods after calibration based on the ASCE (American Society of Civil Engineers)-standardized Penman-Monteith method (the ASCE method includes two reference crops: short-clipped grass and tall alfalfa). The analysis also includes the development of a global grid of revised annual coefficients for solar radiation (Rs) estimations using the respective Rs formula of H-S. The analysis was based on global gridded climatic data of the period 1950-2000. The method for deriving annual coefficients of the P-T and H-S methods was based on partial weighted averages (PWAs) of their mean monthly values. This method estimates the annual values considering the amplitude of the parameter under investigation (ETo and Rs) giving more weight to the monthly coefficients of the months with higher ETo values (or Rs values for the case of the H-S radiation formula). The method also eliminates the effect of unreasonably high or low monthly coefficients that may occur during periods where ETo and Rs fall below a specific threshold. The new coefficients were validated based on data from 140 stations located in various climatic zones of the USA and Australia with expanded observations up to 2016. The validation procedure for ETo estimations of the short reference crop showed that the P-T and H-S methods with the new revised coefficients outperformed the standard methods reducing the estimated root mean square error (RMSE) in ETo values by 40 and 25 %, respectively. The estimations of Rs using the H-S formula with revised coefficients reduced the RMSE by 28 % in comparison to the standard H-S formula. Finally, a raster database was built consisting of (a) global maps for the mean monthly ETo values estimated by ASCE-standardized method for both reference crops, (b) global maps for the revised annual coefficients of the P
24 CFR 200.926e - Supplemental information for use with the CABO One and Two Family Dwelling Code.

Code of Federal Regulations, 2014 CFR

2014-04-01

... shall be in accordance with section 7 of ASCE 7-88. (c) Wind pressures. The minimum Design Wind Pressures (net pressures) set forth below apply to areas designated as experiencing basic wind speeds up to and including 80 mph, as shown in ASCE 7-88, Figure 1, Basic Wind Speed Map. These pressures also...
24 CFR 200.926e - Supplemental information for use with the CABO One and Two Family Dwelling Code.

Code of Federal Regulations, 2013 CFR

2013-04-01

... shall be in accordance with section 7 of ASCE 7-88. (c) Wind pressures. The minimum Design Wind Pressures (net pressures) set forth below apply to areas designated as experiencing basic wind speeds up to and including 80 mph, as shown in ASCE 7-88, Figure 1, Basic Wind Speed Map. These pressures also...
24 CFR 200.926e - Supplemental information for use with the CABO One and Two Family Dwelling Code.

Code of Federal Regulations, 2012 CFR

2012-04-01

... shall be in accordance with section 7 of ASCE 7-88. (c) Wind pressures. The minimum Design Wind Pressures (net pressures) set forth below apply to areas designated as experiencing basic wind speeds up to and including 80 mph, as shown in ASCE 7-88, Figure 1, Basic Wind Speed Map. These pressures also...
24 CFR 200.926e - Supplemental information for use with the CABO One and Two Family Dwelling Code.

Code of Federal Regulations, 2010 CFR

2010-04-01

... shall be in accordance with section 7 of ASCE 7-88. (c) Wind pressures. The minimum Design Wind Pressures (net pressures) set forth below apply to areas designated as experiencing basic wind speeds up to and including 80 mph, as shown in ASCE 7-88, Figure 1, Basic Wind Speed Map. These pressures also...
24 CFR 200.926e - Supplemental information for use with the CABO One and Two Family Dwelling Code.

Code of Federal Regulations, 2011 CFR

2011-04-01

... shall be in accordance with section 7 of ASCE 7-88. (c) Wind pressures. The minimum Design Wind Pressures (net pressures) set forth below apply to areas designated as experiencing basic wind speeds up to and including 80 mph, as shown in ASCE 7-88, Figure 1, Basic Wind Speed Map. These pressures also...
Detection of human papillomavirus 16, 18, and 45 in women with ASC-US cytology and the risk of cervical precancer: results from the CLEAR HPV study.

PubMed

Castle, Phillip E; Cuzick, Jack; Stoler, Mark H; Wright, Thomas C; Reid, Jennifer L; Dockter, Janel; Giachetti, Cristina; Getman, Damon

2015-02-01

The Aptima human papillomavirus (HPV) 16 18/45 Genotype (GT) assay (AHPV-GT) is a qualitative E6/ E7 oncogene messenger RNA test that detects HPV 16 and a pool of HPV 18 and 45. The CLEAR (Clinical Evaluation of APTIMA mRNA) study was the pivotal, prospective, multicenter US clinical study to validate the Aptima HPV (AHPV) assays. In this analysis, we evaluated the clinical performance of AHPV and AHPV-GT assays for detection of cervical intraepithelial neoplasia grade 2 or more severe (CIN2 +) and grade 3 (CIN3) or adenocarcinoma in situ in 912 women with atypical squamous cells of undetermined significance (ASC-US) Papanicolaou result. The AHPV-GT assay was performed on high-risk HPV (hrHPV) positives as determined by the AHPV assay. Overall, the percent positive for hrHPV was 38.8% (354/912), of which 34.2% (121/354) were GT positive. Among hrHPV-positive women, the risks of CIN2 + were 37.0% for HPV 16 positives, 15.9% for HPV 18/45 positives, 14.3% for other hrHPV positives, and 2.2% for AHPV negatives. The risks of CIN3 + were 20.5% for HPV 16 positives, 9.1% for HPV 18/45 positives, 4.3% for other hrHPV positives, and 0.7% for HPV negatives. We demonstrated that AHPV-GT is a reliable and effective test for cervical cancer risk stratification in women with an ASC-US cytology diagnosis. Copyright© by the American Society for Clinical Pathology.
Commencement Bay Studies Phase II, Environmental Impacts Assessment.

DTIC Science & Technology

1983-10-01

Approved for public release, distribution unlimited. 17. DISTRIBUTION STATEMENT (of the absirct entered In Block 20. If dlfforent from Report) IS...Matrix (Appendix D). 19. KEY WORDS (Continue n reveres side itnecsewy and identify by block number) Salmonids Wetlands Aesthetics City of Tacoma Marine...Water Quality Land and Water Use Port of Tacoma t AEINACr (Cm as ,.verem ebb N c evesey a - fdoswif by block n mbs) ames and Moore assessed the
Spirulina platensis Improves Mitochondrial Function Impaired by Elevated Oxidative Stress in Adipose-Derived Mesenchymal Stromal Cells (ASCs) and Intestinal Epithelial Cells (IECs), and Enhances Insulin Sensitivity in Equine Metabolic Syndrome (EMS) Horses

PubMed Central

Nawrocka, Daria; Kornicka, Katarzyna; Śmieszek, Agnieszka

2017-01-01

Equine Metabolic Syndrome (EMS) is a steadily growing life-threatening endocrine disorder linked to insulin resistance, oxidative stress, and systemic inflammation. Inflammatory microenvironment of adipose tissue constitutes the direct tissue milieu for various cell populations, including adipose-derived mesenchymal stromal cells (ASCs), widely considered as a potential therapeutic cell source in the course of the treatment of metabolic disorders. Moreover, elevated oxidative stress induces inflammation in intestinal epithelial cells (IECs)—the first-line cells exposed to dietary compounds. In the conducted research, we showed that in vitro application of Spirulina platensis contributes to the restoration of ASCs’ and IECs’ morphology and function through the reduction of cellular oxidative stress and inflammation. Enhanced viability, suppressed senescence, and improved proliferation of ASCs and IECs isolated from metabolic syndrome-affected individuals were evident following exposition to Spirulina. A protective effect of the investigated extract against mitochondrial dysfunction and degeneration was also observed. Moreover, our data demonstrate that Spirulina extract effectively suppressed LPS-induced inflammatory responses in macrophages. In vivo studies showed that horses fed with a diet based on Spirulina platensis supplementation lost weight and their insulin sensitivity improved. Thus, our results indicate the engagement of Spirulina platensis nourishing as an interesting alternative approach for supporting the conventional treatment of equine metabolic syndrome. PMID:28771165
Development of amnesia in different mouse strains.

PubMed

Sinovyev, D R; Dubrovina, N I; Kulikov, A V

2009-05-01

We studied passive avoidance retrieval after amnestic stimulation (arrest in unsafe section of the experimental setup) in C57Bl/6J, BALB/c, CBA/Lac, AKR/J, DBA/2J, C3H/HeJ, and ASC/Icg mice. We demonstrated resistance to amnestic stimulation in mice with high predisposition to freezing reaction (ASC/Icg) and memory deficit in other mouse strains.
Four new iron meteorite finds

NASA Technical Reports Server (NTRS)

Scott, E. R. D.; Wasson, J. T.; Bild, R. W.

1977-01-01

Four new iron meteorites are described: Buenaventura (IIIB) from Chihuahua, Mexico: mass 114 kg; Denver City (anomalous) from Texas, USA: mass 26.1 kg; Kinsella (IIIB) from Alberta, Canada: mass 3.7 kg; and Tacoma (IA) from Washington, USA: mass 17 g. Denver City is unique - i.e., not related to any other known iron. Tacoma is the smallest iron meteorite recorded. The meteorites were initially discovered in 1969, 1975, 1946, and between 1925 and 1932, respectively.
Granuloma formation and host defense in chronic Mycobacterium tuberculosis infection requires PYCARD/ASC but not NLRP3 or caspase-1.

PubMed

McElvania Tekippe, Erin; Allen, Irving C; Hulseberg, Paul D; Sullivan, Jonathan T; McCann, Jessica R; Sandor, Matyas; Braunstein, Miriam; Ting, Jenny P-Y

2010-08-20

The NLR gene family mediates host immunity to various acute pathogenic stimuli, but its role in chronic infection is not known. This paper addressed the role of NLRP3 (NALP3), its adaptor protein PYCARD (ASC), and caspase-1 during infection with Mycobacterium tuberculosis (Mtb). Mtb infection of macrophages in culture induced IL-1beta secretion, and this requires the inflammasome components PYCARD, caspase-1, and NLRP3. However, in vivo Mtb aerosol infection of Nlrp3(-/-), Casp-1(-/-), and WT mice showed no differences in pulmonary IL-1beta production, bacterial burden, or long-term survival. In contrast, a significant role was observed for Pycard in host protection during chronic Mtb infection, as shown by an abrupt decrease in survival of Pycard(-/-) mice. Decreased survival of Pycard(-/-) animals was associated with defective granuloma formation. These data demonstrate that PYCARD exerts a novel inflammasome-independent role during chronic Mtb infection by containing the bacteria in granulomas.
Antibody-secreting cells in respiratory tract tissues in the absence of eosinophils as supportive partners.

PubMed

Sealy, Robert E; Surman, Sherri L; Vogel, Peter; Hurwitz, Julia L

2016-11-01

Antibody-secreting cells (ASCs) in respiratory tract tissues provide a first line of defense against invading pathogens. These cells often secrete IgA that is efficiently transcytosed across epithelial barriers into the airway lumen where pathogens can be blocked at their point of entry. Previous literature has reported that in the bone marrow, eosinophils are required for the maintenance of ASCs, and that eosinophils co-localize with ASCs as nearest neighbors. To determine if these rules similarly apply to the maintenance of ASCs in respiratory tract tissues, we evaluated virus-specific responses 1 month and 4 months following an intranasal virus infection of eosinophil-null (∆dblGATA-1) mice. Results showed that ASCs were fractionally reduced, but were nonetheless observed in respiratory tract tissues in the absence of eosinophils. Virus-specific antibodies were similarly observed in the airways of eosinophil-deficient mice. Respiratory tract ASCs were also present in mice lacking neutrophils (Mcl1 ∆M ). The staining of tissue sections from the upper respiratory tract of wild-type mice following viral infections demonstrated that virus-specific ASCs were most frequently situated adjacent to epithelial cells rather than eosinophils or neutrophils. Taken together, these data emphasize that rules for cell maintenance are not absolute and that ASCs can survive in the respiratory tract without eosinophils or neutrophils as their nearest neighbors. © The Japanese Society for Immunology. 2016. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Gypsum at Olympia Undae

NASA Technical Reports Server (NTRS)

2006-01-01

This Compact Reconnaissance Imaging Spectrometer for Mars (CRISM) 'targeted image' shows a region of sand dunes surrounding the Martian north polar cap. CRISM, an instrument on NASA's Mars Reconnaissance Orbiter, acquired the image at 1811 UTC (2:11 p.m. EDT) on Oct. 1, 2006. The imaged site is near 80.0 degrees north latitude, 240.7 degrees east longitude. It covers an area about 12 kilometers (7.5 miles) square. At the center of the image, the spatial resolution is as good as 20 meters (65 feet) per pixel. The image was taken in 544 colors covering 0.36 to 3.92 micrometers.
CRISM's sister instrument on the Mars Express spacecraft, OMEGA, has spectrally mapped Mars at lower spatial resolution and discovered that several regions of the planet are rich in sulfate minerals formed by liquid water. Surprisingly, one of the sulfate-rich deposits is a part of the giant field of sand dunes surrounding the north polar cap. CRISM is remapping the dune field at about five times higher resolution than OMEGA, and imaging selected regions at 50 times higher resolution. This image is the first of the high-resolution images of the dune field.
This visualization includes two renderings of the data, both map-projected. The left images are false-color representations showing brightness of the surface at selected infrared wavelengths. The right images show strength of an absorption band at 1900 nanometers wavelength, which indicates the relative abundance of the sulfate mineral gypsum. Brighter areas have more gypsum, and darker areas have less gypsum. The bottom views are enlargements of the central part of the two versions of the image shown at top.
Gypsum is a light-colored, whitish mineral, so it was anticipated that gypsum-rich parts of the sand dunes would be light in color. In fact, there are light-colored areas in the left images, but the images of the gypsum absorption at right show that the light areas have only low gypsum abundance. The dark sand dunes contain most of the gypsum, which is particularly concentrated at the dune crests. CRISM's scientists are taking more high-resolution images of the dune fields to see if this pattern is prevalent, and to attempt to track down the source of the gypsum that makes an arid dune field so rich in minerals formed long ago in liquid water.
The Compact Reconnaissance Imaging Spectrometer for Mars (CRISM) is one of six science instruments on NASA's Mars Reconnaissance Orbiter. Led by The Johns Hopkins University Applied Physics Laboratory, the CRISM team includes expertise from universities, government agencies and small businesses in the United States and abroad.
CRISM's mission: Find the spectral fingerprints of aqueous and hydrothermal deposits and map the geology, composition and stratigraphy of surface features. The instrument will also watch the seasonal variations in Martian dust and ice aerosols, and water content in surface materials -- leading to new understanding of the climate.
NASA's Jet Propulsion Laboratory, a division of the Califonia Institute of Technology, Pasadena, manages the Mars Reconnaissance Orbiter for the NASA Science Mission Directorate, Washington. Lockheed Martin Space Systems, Denver, is the prime contractor and built the spacecraft.
What Do Experienced Water Managers Think of Water Resources of Our Nation and Its Management Infrastructure?

PubMed

Hossain, Faisal; Arnold, Jeffrey; Beighley, Ed; Brown, Casey; Burian, Steve; Chen, Ji; Mitra, Anindita; Niyogi, Dev; Pielke, Roger; Tidwell, Vincent; Wegner, Dave

2015-01-01

This article represents the second report by an ASCE Task Committee "Infrastructure Impacts of Landscape-driven Weather Change" under the ASCE Watershed Management Technical Committee and the ASCE Hydroclimate Technical Committee. Herein, the 'infrastructure impacts" are referred to as infrastructure-sensitive changes in weather and climate patterns (extremes and non-extremes) that are modulated, among other factors, by changes in landscape, land use and land cover change. In this first report, the article argued for explicitly considering the well-established feedbacks triggered by infrastructure systems to the land-atmosphere system via landscape change. In this report by the ASCE Task Committee (TC), we present the results of this ASCE TC's survey of a cross section of experienced water managers using a set of carefully crafted questions. These questions covered water resources management, infrastructure resiliency and recommendations for inclusion in education and curriculum. We describe here the specifics of the survey and the results obtained in the form of statistical averages on the 'perception' of these managers. Finally, we discuss what these 'perception' averages may indicate to the ASCE TC and community as a whole for stewardship of the civil engineering profession. The survey and the responses gathered are not exhaustive nor do they represent the ASCE-endorsed viewpoint. However, the survey provides a critical first step to developing the framework of a research and education plan for ASCE. Given the Water Resources Reform and Development Act passed in 2014, we must now take into account the perceived concerns of the water management community.
What Do Experienced Water Managers Think of Water Resources of Our Nation and Its Management Infrastructure?

PubMed Central

Hossain, Faisal; Arnold, Jeffrey; Beighley, Ed; Brown, Casey; Burian, Steve; Chen, Ji; Mitra, Anindita; Niyogi, Dev; Pielke, Roger; Tidwell, Vincent; Wegner, Dave

2015-01-01

This article represents the second report by an ASCE Task Committee “Infrastructure Impacts of Landscape-driven Weather Change” under the ASCE Watershed Management Technical Committee and the ASCE Hydroclimate Technical Committee. Herein, the ‘infrastructure impacts” are referred to as infrastructure-sensitive changes in weather and climate patterns (extremes and non-extremes) that are modulated, among other factors, by changes in landscape, land use and land cover change. In this first report, the article argued for explicitly considering the well-established feedbacks triggered by infrastructure systems to the land-atmosphere system via landscape change. In this report by the ASCE Task Committee (TC), we present the results of this ASCE TC’s survey of a cross section of experienced water managers using a set of carefully crafted questions. These questions covered water resources management, infrastructure resiliency and recommendations for inclusion in education and curriculum. We describe here the specifics of the survey and the results obtained in the form of statistical averages on the ‘perception’ of these managers. Finally, we discuss what these ‘perception’ averages may indicate to the ASCE TC and community as a whole for stewardship of the civil engineering profession. The survey and the responses gathered are not exhaustive nor do they represent the ASCE-endorsed viewpoint. However, the survey provides a critical first step to developing the framework of a research and education plan for ASCE. Given the Water Resources Reform and Development Act passed in 2014, we must now take into account the perceived concerns of the water management community. PMID:26544045
Human innate immunity to Toxoplasma gondii is mediated by host caspase-1 and ASC and parasite GRA15.

PubMed

Gov, Lanny; Karimzadeh, Alborz; Ueno, Norikiyo; Lodoen, Melissa B

2013-07-09

Interleukin-1β (IL-1β) functions as a key regulator of inflammation and innate immunity. The protozoan parasite Toxoplasma gondii actively infects human blood monocytes and induces the production of IL-1β; however, the host and parasite factors that mediate IL-1β production during T. gondii infection are poorly understood. We report that T. gondii induces IL-1β transcript, processing/cleavage, and release from infected primary human monocytes and THP-1 cells. Treating monocytes with the caspase-1 inhibitor Ac-YVAD-CMK reduced IL-1β release, suggesting a role for the inflammasome in T. gondii-induced IL-1β production. This was confirmed by performing short hairpin RNA (shRNA) knockdown of caspase-1 and of the inflammasome adaptor protein ASC. IL-1β induction required active parasite invasion of monocytes, since heat-killed or mycalolide B-treated parasites did not induce IL-1β. Among the type I, II, and III strains of T. gondii, the type II strain induced substantially more IL-1β mRNA and protein release than did the type I and III strains. Since IL-1β transcript is known to be induced downstream of NF-κB signaling, we investigated a role for the GRA15 protein, which induces sustained NF-κB signaling in a parasite strain-specific manner. By infecting human monocytes with a GRA15-knockout type II strain and a type I strain stably expressing type II GRA15, we determined that GRA15 is responsible for IL-1β induction during T. gondii infection of human monocytes. This research defines a pathway driving human innate immunity by describing a role for the classical inflammasome components caspase-1 and ASC and the parasite GRA15 protein in T. gondii-induced IL-1β production. Monocytes are immune cells that protect against infection by increasing inflammation and antimicrobial activities in the body. Upon infection with the parasitic pathogen Toxoplasma gondii, human monocytes release interleukin-1β (IL-1β), a "master regulator" of inflammation, which
Reflex Human Papillomavirus Test Results as an Option for the Management of Korean Women With Atypical Squamous Cells Cannot Exclude High-Grade Squamous Intraepithelial Lesion

PubMed Central

Ryu, Ki-Jin; Lee, Sanghoon; Min, Kyung-Jin; Kim, Jae Won; Hong, Jin Hwa; Song, Jae Yun; Lee, Jae Kwan

2015-01-01

Background. Current guidelines recommend initial colposcopy with biopsy regardless of human papillomavirus (HPV) test results in women with atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H). The purpose of this study was to evaluate the value of HPV testing in women with ASC-H based on colposcopic pathology results. Materials and Methods. A multicenter cross-sectional study was carried out at three academic hospitals and involved 40,847 Korean women who underwent cervical cancer screening with cytology and HPV tests with or without subsequent colposcopic biopsies between January 2007 and December 2013. Results. ASC-H was diagnosed in 276 women (0.7%). Only 6 of 68 (8.8%) women with ASC-H who were HPV negative had cervical intraepithelial neoplasia grade ≥2 (CIN ≥2) lesions, whereas 47.4% of the women with ASC-H who were HPV positive had CIN ≥2 lesions. No cases of invasive cervical cancer were diagnosed among women with ASC-H who were HPV negative. Logistic regression analysis was performed using the group with normal Papanicolaou test results and HPV-negative status as the reference group. Women with ASC-H who were HPV positive had a significantly increased risk of CIN ≥2 lesions, whereas no significant increase was observed in patients with ASC-H and HPV-negative status. Conclusion. If the result of the HPV test was negative, the risk of CIN ≥2 lesions in Korean women with ASC-H cytology was low. Reflex HPV testing should be an option for the management of women with cytology showing ASC-H to decrease unnecessary colposcopic biopsies, which are expensive and invasive. Implications for Practice: Current American Society for Colposcopy and Cervical Pathology guidelines recommend universal colposcopy for the management of women with atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion (ASC-H) on cytology, regardless of human papillomavirus (HPV) test results. The present study suggested
Red Storm usage model :Version 1.12.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jefferson, Karen L.; Sturtevant, Judith E.

Red Storm is an Advanced Simulation and Computing (ASC) funded massively parallel supercomputer located at Sandia National Laboratories (SNL). The Red Storm Usage Model (RSUM) documents the capabilities and the environment provided for the FY05 Tri-Lab Level II Limited Availability Red Storm User Environment Milestone and the FY05 SNL Level II Limited Availability Red Storm Platform Milestone. This document describes specific capabilities, tools, and procedures to support both local and remote users. The model is focused on the needs of the ASC user working in the secure computing environments at Los Alamos National Laboratory (LANL), Lawrence Livermore National Laboratory (LLNL),more » and SNL. Additionally, the Red Storm Usage Model maps the provided capabilities to the Tri-Lab ASC Computing Environment (ACE) requirements. The ACE requirements reflect the high performance computing requirements for the ASC community and have been updated in FY05 to reflect the community's needs. For each section of the RSUM, Appendix I maps the ACE requirements to the Limited Availability User Environment capabilities and includes a description of ACE requirements met and those requirements that are not met in that particular section. The Red Storm Usage Model, along with the ACE mappings, has been issued and vetted throughout the Tri-Lab community.« less
A psychoanalytic study of Alexander the Great.

PubMed

Thomas, K R

1995-12-01

The purpose of this paper was to demonstrate how Freudian concepts such as the Oedipus complex, castration anxiety, fear of loss of love, the psychosexual stages of development, and the tripartite structure of personality can be used to understand the life and achievements of Alexander the Great. To accomplish this purpose, specific incidents, myths, and relationships in Alexander's life were analyzed from a Freudian psychoanalytic perspective. Green (1991), in his recent biography of Alexander, has questioned the merit of using Freudian concepts to understand Alexander's character. In fact, he stated specifically: If he (Alexander) had any kind of Oedipus complex it came in a poor second to the burning dynastic ambition which Olympias so sedulously fostered in him; those who insist on his psychological motivation would do better to take Adler as their mentor than Freud (p.56). Later, in the concluding section of his book, Green (1991, pp. 486-487) discounted Freudian interpretations of Alexander's distaste for sex, the rumors of his homosexual liaisons, his partiality for middle-aged or elderly ladies, and the systematic domination of his early years by Olympias as little more than the projected fears and desires of the interpreters. And again, an Adlerian power-complex paradigm was suggested as the preferable theoretical framework to use. Green's argument was based primarily on an exchange, reported originally by Plutarch, which took place between Alexander and Philip prior to Alexander's tutorship with Aristotle. Purportedly, Philip enjoined his son to study hard and pay close attention to all Aristotle said "so that you may not do a great many things of the sort that I am sorry I have done." At this point, Alexander "somewhat pertly" took Philip to task "because he was having children by other women besides his wife." Philip's reply was: "Well then, if you have many competitors for the kingdom, prove yourself honorable and good, so that you may obtain the
Safety of gardening on lead- and arsenic-contaminated urban brownfields.

PubMed

Defoe, Phillip P; Hettiarachchi, Ganga M; Benedict, Christopher; Martin, Sabine

2014-11-01

Elevated levels of lead (Pb) and arsenic (As) are not uncommon for urban soils. Test plots were established at urban gardens in Tacoma and Seattle, WA. The Tacoma site was contaminated with Pb (51-312 mg kg) and As (39-146 mg kg), and the Seattle site had high Pb soil concentrations ranging from 506 to 2022 mg kg and As concentrations of <20 mg kg. The efficacy of biosolids mix and compost amendment in reducing Pb and As concentrations in three vegetables (carrots, lettuce, and tomatoes) and the bioaccessibility of soil Pb and As were evaluated. Food-chain transfer of Pb and As were evaluated by measuring plant Pb and As concentrations after kitchen-style washing, a laboratory cleaning procedure, or peeling. The experimental design was a randomized complete block with a split-plot arrangement. Tacoma site treatments included a Class A biosolids mix (TAGRO) with dolomite, and soil at the Seattle site was amended with Cedar-Grove compost (CGC) plus dolomite. TAGRO amendment diluted soil Pb by 10 to 23% and As by 12 to 25% at the Tacoma site, and CGC + dolomite resulted in 20 to 50% dilution in soil Pb at the Seattle site. Both amendments reduced Pb concentrations in vegetables by 50 to 71%, and As reductions ranged from 46 to 80%. At the Tacoma site, Pb concentrations (dry weight basis) in carrots, lettuce, and tomatoes ranged from 8.89 to 25.0, from 0.37 to 3.83, and from 0.54 to 1.24 mg kg, respectively. Plant As concentrations were below 703 μg kg (dry weight) for the vegetables and followed the order lettuce > carrot > tomato. Food-chain transfer of Pb and As in vegetables grown in contaminated urban soils were reduced by laboratory cleaning. Copyright © by the American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America, Inc.
Diverse rupture modes for surface-deforming upper plate earthquakes in the southern Puget Lowland of Washington State

USGS Publications Warehouse

Nelson, Alan R.; Personius, Stephen F.; Sherrod, Brian L.; Kelsey, Harvey M.; Johnson, Samuel Y.; Bradley, Lee-Ann; Wells, Ray E.

2014-01-01

Earthquake prehistory of the southern Puget Lowland, in the north-south compressive regime of the migrating Cascadia forearc, reflects diverse earthquake rupture modes with variable recurrence. Stratigraphy and Bayesian analyses of previously reported and new 14C ages in trenches and cores along backthrust scarps in the Seattle fault zone restrict a large earthquake to 1040–910 cal yr B.P. (2σ), an interval that includes the time of the M 7–7.5 Restoration Point earthquake. A newly identified surface-rupturing earthquake along the Waterman Point backthrust dates to 940–380 cal yr B.P., bringing the number of earthquakes in the Seattle fault zone in the past 3500 yr to 4 or 5. Whether scarps record earthquakes of moderate (M 5.5–6.0) or large (M 6.5–7.0) magnitude, backthrusts of the Seattle fault zone may slip during moderate to large earthquakes every few hundred years for periods of 1000–2000 yr, and then not slip for periods of at least several thousands of years. Four new fault scarp trenches in the Tacoma fault zone show evidence of late Holocene folding and faulting about the time of a large earthquake or earthquakes inferred from widespread coseismic subsidence ca. 1000 cal yr B.P.; 12 ages from 8 sites in the Tacoma fault zone limit the earthquakes to 1050–980 cal yr B.P. Evidence is too sparse to determine whether a large earthquake was closely predated or postdated by other earthquakes in the Tacoma basin, but the scarp of the Tacoma fault was formed by multiple earthquakes. In the northeast-striking Saddle Mountain deformation zone, along the western limit of the Seattle and Tacoma fault zones, analysis of previous ages limits earthquakes to 1200–310 cal yr B.P. The prehistory clarifies earthquake clustering in the central Puget Lowland, but cannot resolve potential structural links among the three Holocene fault zones.
Selenium content in sulfide ores from the Chalkidiki peninsula, Greece.

PubMed

Nicolaidou, A E

1998-01-01

Selenium (Se) was assessed in galena, sphalerite, and pyrite samples. These are components of mixed sulfide ores from the Olympias and Madem Lakkos-Mavres Petres deposits and the Skouries porphyry-copper deposit. We used atomic absorption spectroscopy (AAS) with a hydride generator system. The highest concentration of Se (516 ppm) was found in the fine-grained galena at the -135 level of the Olympias deposits. In the Madem Lakkos-Mavres Petres deposit, the highest concentration of Se (33 ppm) was found in the pyrites of the level 30. The concentration of Se in the arsenopyrites and chalcopyrites is lower than the detection limit of the analytical method (< 100 ppb). The concentrated chalcopyrite from the porphyry copper deposit at Skouries exhibits a significant Se content (average 200 ppm) in contrast to the chalcopyrite from the Olympias and the Madem Lakkos-Mavres Petres. Variations in the Se content of the sulfide minerals studied could be caused by redox-pH and/or temperature conditions, as well as by the difference in crystal structure. The Se found in the areas studied may positively affect the environment. Sulfide minerals are oxidized by microorganisms, infiltrate in the soil-water in the form of selenate or selenite ion, and directly or indirectly influence the human organism.
Interpretation of the Seattle uplift, Washington, as a passive-roof duplex

USGS Publications Warehouse

Brocher, T.M.; Blakely, R.J.; Wells, R.E.

2004-01-01

We interpret seismic lines and a wide variety of other geological and geophysical data to suggest that the Seattle uplift is a passive-roof duplex. A passive-roof duplex is bounded top and bottom by thrust faults with opposite senses of vergence that form a triangle zone at the leading edge of the advancing thrust sheet. In passive-roof duplexes the roof thrust slips only when the floor thrust ruptures. The Seattle fault is a south-dipping reverse fault forming the leading edge of the Seattle uplift, a 40-km-wide fold-and-thrust belt. The recently discovered, north-dipping Tacoma reverse fault is interpreted as a back thrust on the trailing edge of the belt, making the belt doubly vergent. Floor thrusts in the Seattle and Tacoma fault zones, imaged as discontinuous reflections, are interpreted as blind faults that flatten updip into bedding plane thrusts. Shallow monoclines in both the Seattle and Tacoma basins are interpreted to overlie the leading edges of thrust-bounded wedge tips advancing into the basins. Across the Seattle uplift, seismic lines image several shallow, short-wavelength folds exhibiting Quaternary or late Quaternary growth. From reflector truncation, several north-dipping thrust faults (splay thrusts) are inferred to core these shallow folds and to splay upward from a shallow roof thrust. Some of these shallow splay thrusts ruptured to the surface in the late Holocene. Ages from offset soils in trenches across the fault scarps and from abruptly raised shorelines indicate that the splay, roof, and floor thrusts of the Seattle and Tacoma faults ruptured about 1100 years ago.
40 CFR 65.14 - Addresses.

Code of Federal Regulations, 2011 CFR

2011-07-01

... and Ecology, 8001 National Drive, P.O. Box 9583, Little Rock, Arkansas 72209. (5) California. (i... Office Building, Richmond, Virginia 23219. (47) Washington. Department of Ecology, Olympia, Washington...
Dynamo room (compartment A21) with view of port side electrical ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Dynamo room (compartment A-21) with view of port side electrical generator and ventilation ducting. (013) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
77 FR 14586 - Supplemental Environmental Impact Statement; Kittitas County, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-12

... Engineer, South Central Region, Federal Highway Administration, 711 South Capital Way, Suite 501, Olympia... SEIS is limited to the social, economic, and environmental effects of constructing and operating a...
40 CFR 65.14 - Addresses.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Department of Pollution Control and Ecology, 8001 National Drive, P.O. Box 9583, Little Rock, Arkansas 72209... Office Building, Richmond, Virginia 23219. (47) Washington. Department of Ecology, Olympia, Washington...
Treatment efficacy of adipose-derived stem cells in experimental osteoarthritis is driven by high synovial activation and reflected by S100A8/A9 serum levels.

PubMed

Schelbergen, R F; van Dalen, S; ter Huurne, M; Roth, J; Vogl, T; Noël, D; Jorgensen, C; van den Berg, W B; van de Loo, F A; Blom, A B; van Lent, P L E M

2014-08-01

Synovitis is evident in a substantial subpopulation of patients with osteoarthritis (OA) and is associated with development of pathophysiology. Recently we have shown that adipose-derived stem cells (ASC) inhibit joint destruction in collagenase-induced experimental OA (CIOA). In the current study we explored the role of synovitis and alarmins S100A8/A9 in the immunomodulatory capacity of ASCs in experimental OA. CIOA, characterized by synovitis, and surgical DMM (destabilization of medial meniscus) OA were treated locally with ASCs. Synovial activation, cartilage damage and osteophyte size were measured on histological sections. Cytokines in synovial washouts and serum were determined using Luminex or enzyme-linked immunosorbent assay (S100A8/A9), mRNA levels with reverse-transcriptase (RT)-qPCR. Local administration of ASCs at various time-points (days 7 or 14) after DMM induction had no effect on OA pathology. At day 7 of CIOA, already 6 h after ASC injection mRNA expression of pro-inflammatory mediators S100A8/A9, interleukin-1beta (IL-1β) and KC was down-regulated in the synovium. IL-1β protein, although low, was down-regulated by ASC-treatment of CIOA. S100A8/A9 protein levels were very high at 6 and 48 h and were decreased by ASC-treatment. The protective action of ASC treatment in CIOA was only found when high synovial inflammation was present at the time of deposition which was reflected by high serum S100A8/A9 levels. Finally, successful treatment resulted in significantly lower levels of serum S100A8/A9. Our study indicates that synovial activation rapidly drives anti-inflammatory and protective effects of intra-articularly deposited ASCs in experimental OA which is reflected by decreased S100A8/A9 levels. Copyright © 2014 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
Quantitative analysis of injury-induced anterior subcapsular cataract in the mouse: a model of lens epithelial cells proliferation and epithelial-mesenchymal transition.

PubMed

Xiao, Wei; Chen, Xiaoyun; Li, Weihua; Ye, Shaobi; Wang, Wencong; Luo, Lixia; Liu, Yizhi

2015-02-10

The mouse lens capsular injury model has been widely used in investigating the mechanisms of anterior subcapsular cataract (ASC) and posterior capsule opacification (PCO), and evaluating the efficacy of antifibrotic compounds. Nevertheless, there is no available protocol to quantitatively assess the treatment outcomes. Our aim is to describe a new method that can successfully quantify the wound and epithelial-mesenchymal transition (EMT) markers expression in vivo. In this model, lens anterior capsule was punctured with a hypodermic needle, which triggered lens epithelial cells (LECs) proliferation and EMT rapidly. Immunofluorescent staining of injured lens anterior capsule whole-mounts revealed the formation of ASC and high expression of EMT markers in the subcapsular plaques. A series of sectional images of lens capsule were acquired from laser scanning confocal microscopy (LSCM) three-dimensional (3D) scanning. Using LSCM Image Browser software, we can not only obtain high resolution stereo images to present the spatial structures of ASC, but also quantify the subcapsular plaques and EMT markers distribution successfully. Moreover, we also demonstrated that histone deacetylases (HDACs) inhibitor TSA significantly prevented injury-induced ASC using this method. Therefore, the present research provides a useful tool to study ASC and PCO biology as well as the efficacy of new therapies.
Compartment A14m cork lined powder magazine looking port to ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Compartment A-14m - cork lined powder magazine looking port to starboard; note temperature sensor in the overhead. (022) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
77 FR 12792 - Inviting Applications for the Rural Economic Development Loan and Grant Program for Fiscal Year 2012

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-02

... USDA Rural Development State Office, 1835 Black Lake Boulevard SW., Suite B, Olympia, WA 98512-5715... (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and...
Rat model of anal sphincter injury and two approaches for stem cell administration

PubMed Central

Trébol, Jacobo; Georgiev-Hristov, Tihomir; Vega-Clemente, Luz; García-Gómez, Ignacio; Carabias-Orgaz, Ana; García-Arranz, Mariano; García-Olmo, Damián

2018-01-01

.67 s and mean number of peaks was 12.53. Our morphological assessment revealed that with the aforementioned surgical procedure, both sphincters were completely sectioned. In manometry, the described activity disappeared and was replaced by a gentle oscillation of basal line, without a recognizable pattern. Surprisingly, these findings appeared irrespective of injury repair or not. ASCs survived in this potentially septic area for 7 d, at least. We were able to identify them in 84% of animals, mainly in the muscular section area or in the tissue between the muscular endings. ASCs formed a kind of “conglomerate” in rats treated with injections, while in the biosuture group, they wrapped the suture. ASCs were also able to migrate to the damaged zone. No relevant adverse events or mortality could be related to the stem cells in our study. We also did not find unexpected tissue growths. CONCLUSION The proposed procedure produces a consistent sphincter lesion. Biosutures and injections are suitable for cell delivery. ASCs survive and are completely safe in this clinical setting. PMID:29391927

Rat model of anal sphincter injury and two approaches for stem cell administration.

PubMed

Trébol, Jacobo; Georgiev-Hristov, Tihomir; Vega-Clemente, Luz; García-Gómez, Ignacio; Carabias-Orgaz, Ana; García-Arranz, Mariano; García-Olmo, Damián

2018-01-26

peaks was 12.53. Our morphological assessment revealed that with the aforementioned surgical procedure, both sphincters were completely sectioned. In manometry, the described activity disappeared and was replaced by a gentle oscillation of basal line, without a recognizable pattern. Surprisingly, these findings appeared irrespective of injury repair or not. ASCs survived in this potentially septic area for 7 d, at least. We were able to identify them in 84% of animals, mainly in the muscular section area or in the tissue between the muscular endings. ASCs formed a kind of "conglomerate" in rats treated with injections, while in the biosuture group, they wrapped the suture. ASCs were also able to migrate to the damaged zone. No relevant adverse events or mortality could be related to the stem cells in our study. We also did not find unexpected tissue growths. The proposed procedure produces a consistent sphincter lesion. Biosutures and injections are suitable for cell delivery. ASCs survive and are completely safe in this clinical setting.
Sensitivity analysis of the FEMA HAZUS-MH MR4 Earthquake Model using seismic events affecting King County Washington

NASA Astrophysics Data System (ADS)

Neighbors, C.; Noriega, G. R.; Caras, Y.; Cochran, E. S.

2010-12-01

HAZUS-MH MR4 (HAZards U. S. Multi-Hazard Maintenance Release 4) is a risk-estimation software developed by FEMA to calculate potential losses due to natural disasters. Federal, state, regional, and local government use the HAZUS-MH Earthquake Model for earthquake risk mitigation, preparedness, response, and recovery planning (FEMA, 2003). In this study, we examine several parameters used by the HAZUS-MH Earthquake Model methodology to understand how modifying the user-defined settings affect ground motion analysis, seismic risk assessment and earthquake loss estimates. This analysis focuses on both shallow crustal and deep intraslab events in the American Pacific Northwest. Specifically, the historic 1949 Mw 6.8 Olympia, 1965 Mw 6.6 Seattle-Tacoma and 2001 Mw 6.8 Nisqually normal fault intraslab events and scenario large-magnitude Seattle reverse fault crustal events are modeled. Inputs analyzed include variations of deterministic event scenarios combined with hazard maps and USGS ShakeMaps. This approach utilizes the capacity of the HAZUS-MH Earthquake Model to define landslide- and liquefaction- susceptibility hazards with local groundwater level and slope stability information. Where Shakemap inputs are not used, events are run in combination with NEHRP soil classifications to determine site amplification effects. The earthquake component of HAZUS-MH applies a series of empirical ground motion attenuation relationships developed from source parameters of both regional and global historical earthquakes to estimate strong ground motion. Ground motion and resulting ground failure due to earthquakes are then used to calculate, direct physical damage for general building stock, essential facilities, and lifelines, including transportation systems and utility systems. Earthquake losses are expressed in structural, economic and social terms. Where available, comparisons between recorded earthquake losses and HAZUS-MH earthquake losses are used to determine how region
Compartment A123 crews WC (head) looking aft; to forward; note ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Compartment A-123 crews WC (head) looking aft; to forward; note wire lockers for transient stowage of clothing while showering. (026) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
75 FR 33692 - Safety Zone; Tacoma Freedom Fair Air Show, Commencement Bay, Tacoma, WA

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-15

... this rule encompasses all waters within the points 47[deg]-17.63' N., 122[deg]-28.724' W.; 47[deg]-17... Ruston Way and extending approximately 1100 yards into Commencement Bay. Floating markers will be placed... designated safety zone: All waters within the points 47[deg]-17.63' N., 122[deg]-28.724' W.; 47[deg]-17.059...
76 FR 38604 - Inviting Applications for Rural Business Opportunity Grants

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-01

..., 1835 Black Lake Boulevard SW., Suite B, Olympia, WA 98512-5715, (360) 704-7740/TDD (360) 704-7760. West... (braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and...
The Case for Architecture by Committee

ERIC Educational Resources Information Center

Building Design and Construction, 1972

1972-01-01

Multiple-architect designs and a preservative of chain-store identities accomplish visual variety for a one-of-a-kind campus at Evergreen State College in Olympia, Washington, and an enclosed shopping mall in Los Cerritos, California. (Author)
Purple Computational Environment With Mappings to ACE Requirements for the General Availability User Environment Capabilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Barney, B; Shuler, J

2006-08-21

Purple is an Advanced Simulation and Computing (ASC) funded massively parallel supercomputer located at Lawrence Livermore National Laboratory (LLNL). The Purple Computational Environment documents the capabilities and the environment provided for the FY06 LLNL Level 1 General Availability Milestone. This document describes specific capabilities, tools, and procedures to support both local and remote users. The model is focused on the needs of the ASC user working in the secure computing environments at Los Alamos National Laboratory, Lawrence Livermore National Laboratory, and Sandia National Laboratories, but also documents needs of the LLNL and Alliance users working in the unclassified environment. Additionally,more » the Purple Computational Environment maps the provided capabilities to the Trilab ASC Computing Environment (ACE) Version 8.0 requirements. The ACE requirements reflect the high performance computing requirements for the General Availability user environment capabilities of the ASC community. Appendix A lists these requirements and includes a description of ACE requirements met and those requirements that are not met for each section of this document. The Purple Computing Environment, along with the ACE mappings, has been issued and reviewed throughout the Tri-lab community.« less
Ascorbyl stearate and ionizing radiation potentiate apoptosis through intracellular thiols and oxidative stress in murine T lymphoma cells.

PubMed

Mane, Shirish D; Kamatham, Akhilender Naidu

2018-02-01

Ascorbyl stearate (Asc-s) is a derivative of ascorbic acid with better anti-tumour efficacy compared to its parent compound ascorbic acid. In this study, we have examined radio-sensitizing effect of Asc-s in murine T cell lymphoma (EL4) cells at 4 Gy. Asc-s and radiation treatment reduced cell proliferation, induced apoptosis in a dose dependent manner by arresting the cells at S/G2-M phase of cell cycle. It also decreased the frequency of cancer stem cells per se, with significantly higher decrease in combination with radiation treatment./Further, Asc-s and radiation treatment increased the level of reactive oxygen species (ROS), drop in mitochondrial membrane potential (MMP) and increased caspase-3 activity resulting in apoptosis of EL4 cells. Further it also significantly decreased GSH/GSSG ratio due to binding of Asc-s with thiols. The increase in oxidative stress induced by Asc-s and radiation treatment was abrogated by thiol antioxidants in EL4 cells. Interestingly, this redox modulation triggered significant increase in protein glutathionylation in a time dependent manner. Asc-s treatment resulted in glutathionylation of IKK, p50-NF-kB and mutated p53, thereby inhibiting cancer progression during oxidative stress. Asc-s quenches GSH ensuing Asc-s + GSH adduct thereby further modulating GSH/GSSG ratio as evident from HPLC and docking studies. The anti-tumour effect of Asc-s along with radiation was studied by injecting EL4 cells in synegenicC57/BL6 male mice. Intraperitoneal injection of Asc-s followed by radiation exposure at 4 Gy to the tumour bearing mice resulted in radio-sensitization which is evident from significant regression of tumour as evident from tumour burden index. The survival study supports the data that Asc-s pre-treatment enhances radio-sensitization in murine lymphoma. Our data, suggest that Asc-s and ionizing radiation induced cell cycle arrest and apoptosis by perturbing redox balance through irreversible complexes of thiols with Asc-s
Performance and Costs of Ductless Heat Pumps in Marine-Climate High-Performance Homes -- Habitat for Humanity The Woods

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lubliner, Michael; Howard, Luke; Hales, David

The Woods is a Habitat for Humanity (HFH) community of ENERGY STAR Homes Northwest (ESHNW)-certified homes located in the marine climate of Tacoma/Pierce County, Washington. This research report builds on an earlier preliminary draft 2014 BA report, and includes significant billing analysis and cost effectiveness research from a collaborative, ongoing Ductless Heat Pump (DHP)research effort for Tacoma Public Utilities (TPU) and Bonneville Power Administration (BPA). This report focuses on the results of field testing, modeling, and monitoring of ductless mini-split heat pump hybrid heating systems in seven homes built and first occupied at various times between September 2013 and Octobermore » 2014. The report also provides WSU documentation of high-performance home observations, lessons learned, and stakeholder recommendations for builders of affordable high-performance housing such as HFH. Tacoma Public Utilities (TPU) and Bonneville Power Administration (BPA). This report focuses on the results of field testing, modeling, and monitoring of ductless mini-split heat pump hybrid heating systems in seven homes built and first occupied at various times between September 2013 and October 2014. The report also provides WSU documentation of high-performance home observations, lessons learned, and stakeholder recommendations for builders of affordable high-performance housing such as HFH.« less
The Effects of Pre-processing Strategies for Pediatric Cochlear Implant Recipients

PubMed Central

Rakszawski, Bernadette; Wright, Rose; Cadieux, Jamie H.; Davidson, Lisa S.; Brenner, Christine

2016-01-01

Background Cochlear implants (CIs) have been shown to improve children’s speech recognition over traditional amplification when severe to profound sensorineural hearing loss is present. Despite improvements, understanding speech at low-level intensities or in the presence of background noise remains difficult. In an effort to improve speech understanding in challenging environments, Cochlear Ltd. offers pre-processing strategies that apply various algorithms prior to mapping the signal to the internal array. Two of these strategies include Autosensitivity Control™ (ASC) and Adaptive Dynamic Range Optimization (ADRO®). Based on previous research, the manufacturer’s default pre-processing strategy for pediatrics’ everyday programs combines ASC+ADRO®. Purpose The purpose of this study is to compare pediatric speech perception performance across various pre-processing strategies while applying a specific programming protocol utilizing increased threshold (T) levels to ensure access to very low-level sounds. Research Design This was a prospective, cross-sectional, observational study. Participants completed speech perception tasks in four pre-processing conditions: no pre-processing, ADRO®, ASC, ASC+ADRO®. Study Sample Eleven pediatric Cochlear Ltd. cochlear implant users were recruited: six bilateral, one unilateral, and four bimodal. Intervention Four programs, with the participants’ everyday map, were loaded into the processor with different pre-processing strategies applied in each of the four positions: no pre-processing, ADRO®, ASC, and ASC+ADRO®. Data Collection and Analysis Participants repeated CNC words presented at 50 and 70 dB SPL in quiet and HINT sentences presented adaptively with competing R-Space noise at 60 and 70 dB SPL. Each measure was completed as participants listened with each of the four pre-processing strategies listed above. Test order and condition were randomized. A repeated-measures analysis of variance (ANOVA) was used to
7. Photocopy of Blueprint (Original held by the Community end ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Photocopy of Blueprint (Original held by the Community end Economic Development Department, City of Detroit), C. Howard Crane, Architect. FIRST FLOOR PLAN, NOVEMBER 13, 1927. (4' x 5' negative) - Olympia Arena, 5920 Grand River Avenue, Detroit, MI
Research peer exchange, 2014.

DOT National Transportation Integrated Search

2017-08-01

The WSDOT Research Peer Exchange was held in Olympia, Washington on May 13 and 14, 2014 and addressed Research Program and Project Management as described in the following paragraphs: Program Management There are numerous funding programs, standing c...
Periodontal tissue regeneration by combined implantation of adipose tissue-derived stem cells and platelet-rich plasma in a canine model.

PubMed

Tobita, Morikuni; Uysal, Cagri A; Guo, Xin; Hyakusoku, Hiko; Mizuno, Hiroshi

2013-12-01

One goal of periodontal therapy is to regenerate periodontal tissues. Stem cells, growth factors and scaffolds and biomaterials are vital for the restoration of the architecture and function of complex tissues. Adipose tissue-derived stem cells (ASCs) are an ideal population of stem cells for practical regenerative medicine. In addition, platelet-rich plasma (PRP) can be useful for its ability to stimulate tissue regeneration. PRP contains various growth factors and may be useful as a cell carrier in stem cell therapies. The purpose of this study was to determine whether a mixture of ASCs and PRP promoted periodontal tissue regeneration in a canine model. Autologous ASCs and PRP were implanted into areas with periodontal tissue defects. Periodontal tissue defects that received PRP alone or non-implantation were also examined. Histologic, immunohistologic and x-ray studies were performed 1 or 2 months after implantation. The amount of newly formed bone and the scale of newly formed cementum in the region of the periodontal tissue defect were analyzed on tissue sections. The areas of newly formed bone and cementum were greater 2 months after implantation of ASCs and PRP than at 1 month after implantation, and the radiopacity in the region of the periodontal tissue defect increased markedly by 2 months after implantation. The ASCs and PRP group exhibited periodontal tissue with the correct architecture, including alveolar bone, cementum-like structures and periodontal ligament-like structures, by 2 months after implantation. These findings suggest that a combination of autologous ASCs and PRP promotes periodontal tissue regeneration that develops the appropriate architecture for this complex tissue. Copyright © 2013 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
Compartment A125; view forward of barbette, an armored cylinder for ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Compartment A-125; view forward of barbette, an armored cylinder for supporting and protecting the forward 8" gun turret above. Anchor windlass is in left background. (033) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
View looking to starboard of stem powered refrigeration compressor (ice ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View looking to starboard of stem powered refrigeration compressor (ice machine); low counter at left center of photograph is a mold for making block ice. (p55) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
At the Edge of a Polar Cap

NASA Image and Video Library

2014-05-22

The deep chasm that formed on the polar cap edge is identified as an area of strong down-slope winds and has a clear connection to Mars largest dune field, Olympia Undae as observed by NASA Mars Reconnaissance Orbiter.
Compartment A125, view of forward side of anchor windlass and ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Compartment A-125, view of forward side of anchor windlass and ditty box stowage shelves. Ditty boxes were small portable lockers used for storage of crew's personal items. (029) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
NATS truffle and truffle-like fungi 10: Pachyphloeus thysellii sp. nov. (Pezizaceae, Pezizomycotina).

Treesearch

Wes III Colgan; James M. Trappe

2004-01-01

An undescribed truffle found on the Fort Lewis Military Reservation near Olympia, Washington, is described as Pachyphloeus thysellii. This new species, associated with Pseudotsuga menziesii, closely resembles Pachyphloeus prieguensis from southern Europe. It differs from the latter in having yellow veins...
Cigarette Smoking Impairs Adipose Stromal Cell Vasculogenic Activity and Abrogates Potency to Ameliorate Ischemia.

PubMed

Barwinska, Daria; Traktuev, Dmitry O; Merfeld-Clauss, Stephanie; Cook, Todd G; Lu, Hongyan; Petrache, Irina; March, Keith L

2018-06-01

Cigarette smoking (CS) adversely affects the physiologic function of endothelial progenitor, hematopoietic stem and progenitor cells. However, the effect of CS on the ability of adipose stem/stromal cells (ASC) to promote vasculogenesis and rescue perfusion in the context of ischemia is unknown. To evaluate this, ASC from nonsmokers (nCS-ASC) and smokers (CS-ASC), and their activity to promote perfusion in hindlimb ischemia models, as well as endothelial cell (EC) survival and vascular morphogenesis in vitro were assessed. While nCS-ASC improved perfusion in ischemic limbs, CS-ASC completely lost this therapeutic effect. In vitro vasculogenesis assays revealed that human CS-ASC and ASC from CS-exposed mice showed compromised support of EC morphogenesis into vascular tubes, and the CS-ASC secretome was less potent in supporting EC survival/proliferation. Comparative secretome analysis revealed that CS-ASC produced lower amounts of hepatocyte growth factor (HGF) and stromal cell-derived growth factor 1 (SDF-1). Conversely, CS-ASC secreted the angiostatic/pro-inflammatory factor Activin A, which was not detected in nCS-ASC conditioned media (CM). Furthermore, higher Activin A levels were measured in EC/CS-ASC cocultures than in EC/nCS-ASC cocultures. CS-ASC also responded to inflammatory cytokines with 5.2-fold increase in Activin A secretion, whereas nCS-ASC showed minimal Activin A induction. Supplementation of EC/CS-ASC cocultures with nCS-ASC CM or with recombinant vascular endothelial growth factor, HGF, or SDF-1 did not rescue vasculogenesis, whereas inhibition of Activin A expression or activity improved network formation up to the level found in EC/nCS-ASC cocultures. In conclusion, ASC of CS individuals manifest compromised in vitro vasculogenic activity as well as in vivo therapeutic activity. Stem Cells 2018;36:856-867. © 2018 AlphaMed Press.
Localization of ascorbic acid, ascorbic acid oxidase, and glutathione in roots of Cucurbita maxima L.

PubMed

Liso, Rosalia; De Tullio, Mario C; Ciraci, Samantha; Balestrini, Raffaella; La Rocca, Nicoletta; Bruno, Leonardo; Chiappetta, Adriana; Bitonti, Maria Beatrice; Bonfante, Paola; Arrigoni, Oreste

2004-12-01

To understand the function of ascorbic acid (ASC) in root development, the distribution of ASC, ASC oxidase, and glutathione (GSH) were investigated in cells and tissues of the root apex of Cucubita maxima. ASC was regularly distributed in the cytosol of almost all root cells, with the exception of quiescent centre (QC) cells. ASC also occurred at the surface of the nuclear membrane and correspondingly in the nucleoli. No ASC could be observed in vacuoles. ASC oxidase was detected by immunolocalization mainly in cell walls and vacuoles. This enzyme was particularly abundant in the QC and in differentiating vascular tissues and was absent in lateral root primordia. Administration of the ASC precursor L-galactono-gamma-lactone markedly increased ASC content in all root cells, including the QC. Root treatment with the ASC oxidized product, dehydroascorbic acid (DHA), also increased ASC content, but caused ASC accumulation only in peripheral tissues, where DHA was apparently reduced at the expense of GSH. The different pattern of distribution of ASC in different tissues and cell compartments reflects its possible role in cell metabolism and root morphogenesis.

ASC ATDM Level 2 Milestone #5325: Asynchronous Many-Task Runtime System Analysis and Assessment for Next Generation Platforms.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Baker, Gavin Matthew; Bettencourt, Matthew Tyler; Bova, Steven W.

2015-09-01

This report provides in-depth information and analysis to help create a technical road map for developing next- generation Orogramming mocleN and runtime systemsl that support Advanced Simulation and Computing (ASC) work- load requirements. The focus herein is on 4synchronous many-task (AMT) model and runtime systems, which are of great interest in the context of "Oriascale7 computing, as they hold the promise to address key issues associated with future extreme-scale computer architectures. This report includes a thorough qualitative and quantitative examination of three best-of-class AIM] runtime systemsHCharm-HE, Legion, and Uintah, all of which are in use as part of the Centers.more » The studies focus on each of the runtimes' programmability, performance, and mutability. Through the experiments and analysis presented, several overarching Predictive Science Academic Alliance Program II (PSAAP-II) Ascl findings emerge. From a performance perspective, AIVT11runtimes show tremendous potential for addressing extreme- scale challenges. Empirical studies show an AM11 runtime can mitigate performance heterogeneity inherent to the machine itself and that Message Passing Interface (MP1) and AM11runtimes perform comparably under balanced con- ditions. From a programmability and mutability perspective however, none of the runtimes in this study are currently ready for use in developing production-ready Sandia ASCIapplications. The report concludes by recommending a co- design path forward, wherein application, programming model, and runtime system developers work together to define requirements and solutions. Such a requirements-driven co-design approach benefits the community as a whole, with widespread community engagement mitigating risk for both application developers developers. and high-performance computing inntime systein« less
12 CFR 1102.306 - Procedures for requesting records.

Code of Federal Regulations, 2011 CFR

2011-01-01

... section; (B) Where the running of such time is suspended for the calculation of a cost estimate for the... section; (C) Where the running of such time is suspended for the payment of fees pursuant to the paragraph... of the invoice. (ix) The time limit for the ASC to respond to a request will not begin to run until...
Adipose tissue-derived stem cells inhibit neointimal formation in a paracrine fashion in rat femoral artery.

PubMed

Takahashi, Masao; Suzuki, Etsu; Oba, Shigeyoshi; Nishimatsu, Hiroaki; Kimura, Kenjiro; Nagano, Tetsuo; Nagai, Ryozo; Hirata, Yasunobu

2010-02-01

Subcutaneous adipose tissue contains a lot of stem cells [adipose-derived stem cells (ASCs)] that can differentiate into a variety of cell lineages. In this study, we isolated ASCs from Wistar rats and examined whether ASCs would efficiently differentiate into vascular endothelial cells (ECs) in vitro. We also administered ASCs in a wire injury model of rat femoral artery and examined their effects. ASCs expressed CD29 and CD90, but not CD34, suggesting that ASCs resemble bone marrow-derived mesenchymal stem cells. When induced to differentiate into ECs with endothelial growth medium (EGM), ASCs expressed Flt-1, but not Flk-1 or mature EC markers such as CD31 and vascular endothelial cadherin. ASCs produced angiopoietin-1 when they were cultured in EGM. ASCs stimulated the migration of EC, as assessed by chemotaxis assay. When ASCs that were cultured in EGM were injected in the femoral artery, the ASCs potently and significantly inhibited neointimal formation without being integrated in the endothelial layer. EGM-treated ASCs significantly suppressed neointimal formation even when they were administered from the adventitial side. ASC administration significantly promoted endothelial repair. These results suggested that although ASCs appear to have little capacity to differentiate into mature ECs, ASCs have the potential to secrete paracrine factors that stimulate endothelial repair. Our results also suggested that ASCs inhibited neointimal formation via their paracrine effect of stimulation of EC migration in situ rather than the direct integration into the endothelial layer.
View port to starboard of compartment B127, warrant officers mess ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View port to starboard of compartment B-127, warrant officers mess room. Note sideboard, table and paneling. Port holes to engine room skylight area are on left of photograph. (086) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
Factors preventing the recovery of a historically overexploited shellfish species, Ostrea conchaphila

USDA-ARS?s Scientific Manuscript database

The native oyster in estuaries along the Pacific coast of North America, Ostrea conchaphila (prev. Ostrea lurida, Olympia oyster), experienced overexploitation throughout its range in the late 1800’s, resulting in commercial extinction before 1930. Significant harvest restrictions and marine reserv...
View aft of compartment D23, aft steering station; note steering ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View aft of compartment D-23, aft steering station; note steering unit with crosshead and shaft bearing supports. Note framing supports for armored protective deck at top of photo. (p60) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
8. Photocopy of Blueprint (Original held by the Community and ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

8. Photocopy of Blueprint (Original held by the Community and Economic Development Department, City of Detroit), C. Howard Crane, Architect. GRAND RIVER AVENUE ELEVATION AND MCGRAW AVENUE ELEVATION, NOVEMBER 13, 1927. (4' x 5' negative) - Olympia Arena, 5920 Grand River Avenue, Detroit, MI
Upregulation of CC Chemokine Receptor 7 (CCR7) Enables Migration of Xenogeneic Human Adipose-Derived Mesenchymal Stem Cells to Rat Secondary Lymphoid Organs.

PubMed

Ma, Tian; Luan, Shao-Liang; Huang, Hong; Sun, Xing-Kun; Yang, Yan-Mei; Zhang, Hui; Han, Wei-Dong; Li, Hong; Han, Yan

2016-12-30

BACKGROUND CC chemokine receptor 7 (CCR7) expression is vital for cell migration to secondary lymphoid organs (SLOs). Our previous work showed that inducing CCR7 expression enabled syngeneic mesenchymal stem cells (MSCs) to migrate into SLOs, resulting in enhanced immunosuppressive performance in mice. Given that human adipose-derived stem cells (hASCs) are widely used in clinical therapy, we further investigated whether upregulation of CCR7 enables xenogeneic hASCs to migrate to rat SLOs. MATERIAL AND METHODS hASCs rarely express CCR7; therefore, hASCs were transfected with lentivirus encoding rat CCR7 (rCCR7) plus green fluorescence protein (GFP) or GFP alone. CCR7 mRNA and cell surface expression of rCCR7-hASCs and GFP-hASCs were examined by reverse transcription-polymerase chain reaction (RT-PCR) and flow cytometry (FCM), respectively. The phenotype, differentiation, and proliferation capacity of each cell type was also determined. To examine migration, rCCR7-hASCs and GFP-hASCs were injected intravenously into Lewis rats, and the proportion of GFP-positive cells in the spleen and lymph nodes was determined with FCM. RESULTS mRNA and cell surface protein expression of CCR7 was essentially undetectable in hASCs and GFP-ASCs; however, CCR7 was highly expressed in rCCR7-ASCs. rCCR7-hASCs, GFP-hASCs, and hASCs shared a similar immunophenotype, and maintained the ability of multilineage differentiation and proliferation. In addition, the average proportion of GFP-positive cells was significantly higher following transplantation of rCCR7-hASCs compared with GFP-hASCs (p<0.01). CONCLUSIONS These results suggest that upregulation of rat CCR7 expression does not change the phenotype, differentiation, or proliferation capacity of hASCs, but does enable efficient migration of hASCs to rat SLOs.
Fitness, Fatness and Active School Commuting among Liverpool Schoolchildren

PubMed Central

Boddy, Lynne M.; Knowles, Zoe R.; Fairclough, Stuart J.

2017-01-01

This study investigated differences in health outcomes between active and passive school commuters, and examined associations between parent perceptions of the neighborhood environment and active school commuting (ASC). One hundred-ninety-four children (107 girls), aged 9–10 years from ten primary schools in Liverpool, England, participated in this cross-sectional study. Measures of stature, body mass, waist circumference and cardiorespiratory fitness (CRF) were taken. School commute mode (active/passive) was self-reported and parents completed the neighborhood environment walkability scale for youth. Fifty-three percent of children commuted to school actively. Schoolchildren who lived in more deprived neighborhoods perceived by parents as being highly connected, unaesthetic and having mixed land-use were more likely to commute to school actively (p < 0.05). These children were at greatest risk of being obese and aerobically unfit (p < 0.01). Our results suggest that deprivation may explain the counterintuitive relationship between obesity, CRF and ASC in Liverpool schoolchildren. These findings encourage researchers and policy makers to be equally mindful of the social determinants of health when advocating behavioral and environmental health interventions. Further research exploring contextual factors to ASC, and examining the concurrent effect of ASC and diet on weight status by deprivation is needed. PMID:28858268
Ca2+-mediated ascorbate release from coronary artery endothelial cells.

PubMed

Davis, Kim A; Samson, Sue E; Best, Kelly; Mallhi, Kanwaldeep K; Szewczyk, Magdalena; Wilson, John X; Kwan, Chiu-Yin; Grover, Ashok K

2006-01-01

1.--The addition of Ca(2+) ionophore A23187 or ATP to freshly isolated or cultured pig coronary artery endothelial cells (PCEC) potentiated the release of ascorbate (Asc). Cultured PCEC were used to characterize the Ca(2+)-mediated release. An increase in Ca(2+)-mediated Asc release was observed from PCEC preincubated with Asc, Asc-2-phosphate or dehydroascorbic acid (DHAA). 2.--The effects of various ATP analogs and inhibition by suramin were consistent with the ATP-induced release being mediated by P2Y2-like receptors. 3.--ATP-stimulated Asc release was Ca(2+)-mediated because (a) ATP analogs that increased Asc release also elevated cytosolic [Ca(2+)], (b) Ca(2+) ionophore A23187 and cyclopiazonic acid stimulated the Asc release, (c) removing extracellular Ca(2+) and chelating intracellular Ca(2+)inhibited the ATP-induced release, and (d) inositol-selective phospholipase C inhibitor U73122 also inhibited this release. 4.--Accumulation of Asc by PCEC was examined at Asc concentrations of 10 microM (Na(+)-Asc symporter not saturated) and 5 mM (Na(+)-Asc symporter saturated). At 10 microM Asc, A23187 and ATP caused an inhibition of Asc accumulation but at 5 mM Asc, both the agents caused a stimulation. Substituting gluconate for chloride did not affect the basal Asc uptake but it abolished the effects of A23187. 5.--PCEC but not pig coronary artery smooth muscle cells show a Ca(2+)- mediated Asc release pathway that may be activated by agents such as ATP.
Advanced Stirling Convertor Development for NASA Radioisotope Power Systems

NASA Technical Reports Server (NTRS)

Wong, Wayne A.; Wilson, Scott D.; Collins, Josh

2015-01-01

Sunpower Inc.'s Advanced Stirling Convertor (ASC) initiated development under contract to the NASA Glenn Research Center and after a series of successful demonstrations, the ASC began transitioning from a technology development project to a flight development project. The ASC has very high power conversion efficiency making it attractive for future Radioisotope Power Systems (RPS) in order to make best use of the low plutonium-238 fuel inventory in the United States. In recent years, the ASC became part of the NASA and Department of Energy (DOE) Advanced Stirling Radioisotope Generator (ASRG) Integrated Project. Sunpower held two parallel contracts to produce ASCs, one with the DOE and Lockheed Martin to produce the ASC-F flight convertors, and one with NASA Glenn for the production of ASC-E3 engineering units, the initial units of which served as production pathfinders. The integrated ASC technical team successfully overcame various technical challenges that led to the completion and delivery of the first two pairs of flightlike ASC-E3 by 2013. However, in late fall 2013, the DOE initiated termination of the Lockheed Martin ASRG flight development contract driven primarily by budget constraints. NASA continues to recognize the importance of high-efficiency ASC power conversion for RPS and continues investment in the technology including the continuation of ASC-E3 production at Sunpower and the assembly of the ASRG Engineering Unit #2. This paper provides a summary of ASC technical accomplishments, overview of tests at Glenn, plans for continued ASC production at Sunpower, and status of Stirling technology development.
Ascorbate metabolism and the developmental demand for tartaric and oxalic acids in ripening grape berries

PubMed Central

2009-01-01

Background Fresh fruits are well accepted as a good source of the dietary antioxidant ascorbic acid (Asc, Vitamin C). However, fruits such as grapes do not accumulate exceptionally high quantities of Asc. Grapes, unlike most other cultivated fruits do however use Asc as a precursor for the synthesis of both oxalic (OA) and tartaric acids (TA). TA is a commercially important product in the wine industry and due to its acidifying effect on crushed juice it can influence the organoleptic properties of the wine. Despite the interest in Asc accumulation in fruits, little is known about the mechanisms whereby Asc concentration is regulated. The purpose of this study was to gain insights into Asc metabolism in wine grapes (Vitis vinifera c.v. Shiraz.) and thus ascertain whether the developmental demand for TA and OA synthesis influences Asc accumulation in the berry. Results We provide evidence for developmentally differentiated up-regulation of Asc biosynthetic pathways and subsequent fluctuations in Asc, TA and OA accumulation. Rapid accumulation of Asc and a low Asc to dehydroascorbate (DHA) ratio in young berries was co-ordinated with up-regulation of three of the primary Asc biosynthetic (Smirnoff-Wheeler) pathway genes. Immature berries synthesised Asc in-situ from the primary pathway precursors D-mannose and L-galactose. Immature berries also accumulated TA in early berry development in co-ordination with up-regulation of a TA biosynthetic gene. In contrast, ripe berries have up-regulated expression of the alternative Asc biosynthetic pathway gene D-galacturonic acid reductase with only residual expression of Smirnoff-Wheeler Asc biosynthetic pathway genes and of the TA biosynthetic gene. The ripening phase was further associated with up-regulation of Asc recycling genes, a secondary phase of increased accumulation of Asc and an increase in the Asc to DHA ratio. Conclusion We demonstrate strong developmental regulation of Asc biosynthetic, recycling and catabolic
Northwest Ports Achievements in Reducing Emissions and Improving Performance

EPA Pesticide Factsheets

Case history on the status of the Northwest Ports Clean Air Strategy for the Port of Seattle, Port of Tacoma, and Port Metro Vancouver. Presents key objectives, emission and performance goals and outcomes.
Compartment A20 construction stories locker looking from forward to aft ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Compartment A-20 construction stories locker looking from forward to aft showing wooden ladder and storage bins. Heavy frame supports armored protective deck. Armor plate hatch at top left penetrates protective deck. (05) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
Distribution and Demographics of Marine Mammals in SOCAL Through Photo-Identification, Genetics, and Satellite Telemetry

DTIC Science & Technology

2014-12-01

and analysis, we thank Sabre Mahaffy, Erin Keene, Eric Keen, and Alex Zerbini. For assistance in the field, we thank Jeff Foster and the crew from...McSweeney Wild Whale Research Foundation Holualoa, HI Sabre D. Mahaffy Cascadia Research Collective Olympia, WA Jessica M. Aschettino
Leptin produced by obese adipose stromal/stem cells enhances proliferation and metastasis of estrogen receptor positive breast cancers.

PubMed

Strong, Amy L; Ohlstein, Jason F; Biagas, Brandi A; Rhodes, Lyndsay V; Pei, Dorothy T; Tucker, H Alan; Llamas, Claire; Bowles, Annie C; Dutreil, Maria F; Zhang, Shijia; Gimble, Jeffrey M; Burow, Matthew E; Bunnell, Bruce A

2015-08-19

The steady increase in the incidence of obesity among adults has been paralleled with higher levels of obesity-associated breast cancer. While recent studies have suggested that adipose stromal/stem cells (ASCs) isolated from obese women enhance tumorigenicity, the mechanism(s) by which this occurs remains undefined. Evidence suggests that increased adiposity results in increased leptin secretion from adipose tissue, which has been shown to increased cancer cell proliferation. Previously, our group demonstrated that ASCs isolated from obese women (obASCs) also express higher levels of leptin relative to ASCs isolated from lean women (lnASCs) and that this obASC-derived leptin may account for enhanced breast cancer cell growth. The current study investigates the impact of inhibiting leptin expression in lnASCs and obASCs on breast cancer cell (BCC) growth and progression. Estrogen receptor positive (ER+) BCCs were co-cultured with leptin shRNA lnASCs or leptin shRNA obASCs and changes in the proliferation, migration, invasion, and gene expression of BCCs were investigated. To assess the direct impact of leptin inhibition in obASCs on BCC proliferation, MCF7 cells were injected alone or mixed with control shRNA obASCs or leptin shRNA obASCs into SCID/beige mice. ER+ BCCs were responsive to obASCs during direct co-culture, whereas lnASCs were unable to increase ER(+) BCC growth. shRNA silencing of leptin in obASCs negated the enhanced proliferative effects of obASC on BCCs following direct co-culture. BCCs co-cultured with obASCs demonstrated enhanced expression of epithelial-to-mesenchymal transition (EMT) and metastasis genes (SERPINE1, MMP-2, and IL-6), while BCCs co-cultured with leptin shRNA obASCs did not display similar levels of gene induction. Knockdown of leptin significantly reduced tumor volume and decreased the number of metastatic lesions to the lung and liver. These results correlated with reduced expression of both SERPINE1 and MMP-2 in tumors formed
Human platelet lysate as a fetal bovine serum substitute improves human adipose-derived stromal cell culture for future cardiac repair applications.

PubMed

Naaijkens, B A; Niessen, H W M; Prins, H-J; Krijnen, P A J; Kokhuis, T J A; de Jong, N; van Hinsbergh, V W M; Kamp, O; Helder, M N; Musters, R J P; van Dijk, A; Juffermans, L J M

2012-04-01

Adipose-derived stromal cells (ASC) are promising candidates for cell therapy, for example to treat myocardial infarction. Commonly, fetal bovine serum (FBS) is used in ASC culturing. However, FBS has several disadvantages. Its effects differ between batches and, when applied clinically, transmission of pathogens and antibody development against FBS are possible. In this study, we investigated whether FBS can be substituted by human platelet lysate (PL) in ASC culture, without affecting functional capacities particularly important for cardiac repair application of ASC. We found that PL-cultured ASC had a significant 3-fold increased proliferation rate and a significantly higher attachment to tissue culture plastic as well as to endothelial cells compared with FBS-cultured ASC. PL-cultured ASC remained a significant 25% smaller than FBS-cultured ASC. Both showed a comparable surface marker profile, with the exception of significantly higher levels of CD73, CD90, and CD166 on PL-cultured ASC. PL-cultured ASC showed a significantly higher migration rate compared with FBS-cultured ASC in a transwell assay. Finally, FBS- and PL-cultured ASC had a similar high capacity to differentiate towards cardiomyocytes. In conclusion, this study showed that culturing ASC is more favorable in PL-supplemented medium compared with FBS-supplemented medium.
29. TRACK LAYOUT, INDEX TO DRAWINGS AND INDEX TO MATERIALS, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

29. TRACK LAYOUT, INDEX TO DRAWINGS AND INDEX TO MATERIALS, REED & STEM ARCHITECTS, ST. PAUL, NEW YORK, 1909 (Burlington Northern Collection, Seattle, Washington) - Union Passenger Station Concourse, 1713 Pacific Avenue, Tacoma, Pierce County, WA
78 FR 73881 - National Register of Historic Places; Notification of Pending Nominations and Related Actions

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-09

... review, we cannot guarantee that we will be able to do so. Dated: November 21, 2013. J. Paul Loether... Central Elementary School, 401 S. 8th St., Tacoma, 13000998 Spokane County Garland Theater, (Movie...
Teaching psychosocial aspects of coronary care.

PubMed

Egnew, T R; Jones, J M

1984-01-01

For the past two years, Tacoma Family Medicine, Tacoma, Washington. has integrated a curriculum in psychosocial aspects of coronary care with the Coronary Care Unit rotation taken by third-year residents. Goals of the curriculum are to provide information, to foster the development of psychosocial strategies for patient and family care, and to develop greater insight and awareness on the part of residents regarding the psychosocial aspects of coronary care. Attempts are made to meet these goals in a context as closely tied to the clinical management of patients and families as possible. Topics addressed include the biopsychosocial medical model, patient and family responses to cardiac illness, sexual concerns of the coronary patient and spouse, and issues related to cardiac rehabilitation. The format for the curriculum involves didactic presentations, group discussions, and case consultations.

Thickness of unconsolidated deposits of the Puget Sound aquifer system, Washington and British Columbia

USGS Publications Warehouse

Jones, M.A.

1996-01-01

The Puget-Willamette Lowland is located in western Washington, western Oregon, and a small part of southwestern British Columbia, Canada. The Puget-Willamette Lowland study area is composed of two distinct subareas, the Puget Sound Lowland and the Willamette Lowland. This report presents the results of mapping the thickness of the unconsolidated deposits in the Puget Sound Lowland. The thickness of the unconsolidated deposits ranges from a discontinuous veneer in areas of bedrock outcrop to more than 3,600 feet. Available information shows that the unconsolidated deposits are thickest in the Fraser-Whatcom, Everett, Seattle, and Tacoma Basins. The mapped thickness of the unconsolidated deposits in the Tacoma Basin is probably underestimated because of the scarcity of wells penetrating the full thickness of the unconsolidated deposits and the lack of sufficient marine-seismic data.
Increase in tartrate-resistant acid phosphatase of bone at the early stage of ascorbic acid deficiency in the ascorbate-requiring Osteogenic Disorder Shionogi (ODS) rat.

PubMed

Goto, A; Tsukamoto, I

2003-08-01

The effect of ascorbic acid deficiency on bone metabolism was evaluated using the ascorbate-requiring Osteogenic Disorder Shionogi (ODS) rat model. Ascorbic acid (Asc)-deficient rats gained body weight in a manner similar to Asc-supplemented rats (control) during 3 weeks, but began to lose weight during the 4th week of Asc deficiency. The tartrate-resistant acid phosphatase (TRAP) activity in serum increased to about 2-fold the control value in the rats fed the Asc-free diet for 2, 3, and 4 weeks (AscD2, AscD3, and AscD4), while a decrease in the alkaline phosphatase (ALP) activity was observed only in AscD4 rats. The serum pyridinoline cross-linked carboxyterminal telopeptide of type I collagen (ICTP) level significantly increased to 1.3-, 1.4-, and 1.9-fold of that in the controls in AscD2, D3, and D4, respectively. The ALP activity in the distal femur was unchanged in AscD1, D2, and D3, but decreased to 50% of the control level in AscD4 rats. The TRAP activity in the distal femur increased to about 2-fold of that in the controls in the AscD2 and D3 and decreased to the control level in the AscD4 rats. The amount of hydroxyproline in the distal femur significantly decreased to about 80%, 70%, and 60% of the control in AscD2, D3, and D4 rats, respectively. These decreases were associated with a similar reduction in the calcium content of the distal femur. Histochemical analysis of the distal femur showed an increase in TRAP-positive cells in AscD2 and AscD3 rats and a decrease in the trabecular bone in AscD2, D3, and D4 rats. These results suggested that a deficiency of Asc stimulated bone resorption at an early stage, followed by a decrease in bone formation in mature ODS rats which already had a well-developed collagen matrix and fully differentiated osteoblasts.
Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome.

PubMed

Clark, Kaitlin C; Fierro, Fernando A; Ko, Emily Mills; Walker, Naomi J; Arzi, Boaz; Tepper, Clifford G; Dahlenburg, Heather; Cicchetto, Andrew; Kol, Amir; Marsh, Lyndsey; Murphy, William J; Fazel, Nasim; Borjesson, Dori L

2017-03-20

Adipose-derived mesenchymal stem cells (ASCs) are a promising cell therapy to treat inflammatory and immune-mediated diseases. Development of appropriate pre-clinical animal models is critical to determine safety and attain early efficacy data for the most promising therapeutic candidates. Naturally occurring diseases in cats already serve as valuable models to inform human clinical trials in oncologic, cardiovascular, and genetic diseases. The objective of this study was to complete a comprehensive side-by-side comparison of human and feline ASCs, with an emphasis on their immunomodulatory capacity and transcriptome. Human and feline ASCs were evaluated for phenotype, immunomodulatory profile, and transcriptome. Additionally, transwells were used to determine the role of cell-cell contact in ASC-mediated inhibition of lymphocyte proliferation in both humans and cats. Similar to human ASCs, feline ASCs were highly proliferative at low passages and fit the minimal criteria of multipotent stem cells including a compatible surface protein phenotype, osteogenic capacity, and normal karyotype. Like ASCs from all species, feline ASCs inhibited mitogen-activated lymphocyte proliferation in vitro, with or without direct ASC-lymphocyte contact. Feline ASCs mimic human ASCs in their mediator secretion pattern, including prostaglandin E2, indoleamine 2,3 dioxygenase, transforming growth factor beta, and interleukin-6, all augmented by interferon gamma secretion by lymphocytes. The transcriptome of three unactivated feline ASC lines were highly similar. Functional analysis of the most highly expressed genes highlighted processes including: 1) the regulation of apoptosis; 2) cell adhesion; 3) response to oxidative stress; and 4) regulation of cell differentiation. Finally, feline ASCs had a similar gene expression profile to noninduced human ASCs. Findings suggest that feline ASCs modulate lymphocyte proliferation using soluble mediators that mirror the human ASC secretion
Cognition in Males and Females with Autism: Similarities and Differences

PubMed Central

Lai, Meng-Chuan; Lombardo, Michael V.; Ruigrok, Amber N. V.; Chakrabarti, Bhismadev; Wheelwright, Sally J.; Auyeung, Bonnie; Allison, Carrie; Baron-Cohen, Simon

2012-01-01

The male bias in autism spectrum conditions (ASC) has led to females with ASC being under-researched. This lack of attention to females could hide variability due to sex that may explain some of the heterogeneity within ASC. In this study we investigate four key cognitive domains (mentalizing and emotion perception, executive function, perceptual attention to detail, and motor function) in ASC, to test for similarities and differences between males and females with and without ASC (n = 128 adults; n = 32 per group). In the mentalizing and facial emotion perception domain, males and females with ASC showed similar deficits compared to neurotypical controls. However, in attention to detail and dexterity involving executive function, although males with ASC showed poorer performance relative to neurotypical males, females with ASC performed comparably to neurotypical females. We conclude that performance in the social-cognitive domain is equally impaired in male and female adults with ASC. However, in specific non-social cognitive domains, performance within ASC depends on sex. This suggests that in specific domains, cognitive profiles in ASC are modulated by sex. PMID:23094036
20. VIEW OF EAST END OF SPERRY OCEAN DOCK, SHOWING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

20. VIEW OF EAST END OF SPERRY OCEAN DOCK, SHOWING SOUTH AND EAST ELEVATION, INCLUDING PORTION OF THE SPERRY OCEAN DOCK DECK, LOOKING NORTH - Puget Sound Flouring Mills, 611 Schuster Parkway, Tacoma, Pierce County, WA
75 FR 40816 - Sunshine Act Meeting Notice

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-14

... Matters, Reliability, Security and Market Operations A-3 AD10-15-000...... Smart Grid Update...-2 UL09-1-002....... L.S. Starrett Company. H-3 P-460-033........ City of Tacoma, Washington. P-460...
View forward from secondary bridge; note stack for venting after ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View forward from secondary bridge; note stack for venting after boilers, ventilators, davits, searchlights on port and starboard stanchions and ship's pulling boats; skylight at lower center provides light to engine room. (p30) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
View forward in starboard engine room, compartment C1. Note starboard ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View forward in starboard engine room, compartment C-1. Note starboard engine thrust bearing in open housing at bottom center of photograph; note main circulation pump, main steam chest at top of photo. (065) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
The innovation process centre stage.

PubMed

2005-09-01

London's Olympia 2 was the venue for the first Medical Device Technology Innovation Forum. The event was characterised by dialogue and discussion that explored the key issues involved in delivering clinical need through innovative products to the end user. Highlights of the event are reported here.
Combined introduction of Bmi-1 and hTERT immortalizes human adipose tissue-derived stromal cells with low risk of transformation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tatrai, Peter, E-mail: peter.tatrai@biomembrane.hu; Department of Biochemistry and Molecular Biology, Medical and Health Science Center, University of Debrecen, Egyetem ter 1, H-4032 Debrecen; Szepesi, Aron, E-mail: aron.szepesi@biomembrane.hu

2012-05-25

Highlights: Black-Right-Pointing-Pointer We immortalized human adipose stromal cells (ASCs) with hTERT, Bmi-1, and SV40T. Black-Right-Pointing-Pointer hTERT-only ASCs are prone to transformation, while Bmi-only ASCs become senescent. Black-Right-Pointing-Pointer SV40T introduced along with hTERT abrogates proliferation control and multipotency. Black-Right-Pointing-Pointer hTERT combined with Bmi-1 yields stable phenotype up to 140 population doublings. -- Abstract: Adipose tissue-derived stromal cells (ASCs) are increasingly being studied for their usefulness in regenerative medicine. However, limited life span and donor-dependent variation of primary cells such as ASCs present major hurdles to controlled and reproducible experiments. We therefore aimed to establish immortalized ASC cell lines that provide steadymore » supply of homogeneous cells for in vitro work while retain essential features of primary cells. To this end, combinations of human telomerase reverse transcriptase (hTERT), murine Bmi-1, and SV40 large T antigen (SV40T) were introduced by lentiviral transduction into ASCs. The resulting cell lines ASC{sup hTERT}, ASC{sup Bmi-1}, ASC{sup Bmi-1+hTERT} and ASC{sup SV40T+hTERT} were tested for transgene expression, telomerase activity, surface immunomarkers, proliferation, osteogenic and adipogenic differentiation, karyotype, tumorigenicity, and cellular senescence. All cell lines have maintained expression of characteristic surface immunomarkers, and none was tumorigenic. However, ASC{sup Bmi-1} had limited replicative potential, while the rapidly proliferating ASC{sup SV40T+hTERT} acquired chromosomal aberrations, departed from MSC phenotype, and lost differentiation capacity. ASC{sup hTERT} and ASC{sup hTERT+Bmi-1}, on the other hand, preserved all essential MSC features and did not senesce after 100 population doublings. Notably, a subpopulation of ASC{sup hTERT} also acquired aberrant karyotype and showed signs of transformation after long
76 FR 20807 - Notice of Final Federal Agency Actions on the Sellwood Bridge Project, SE Tacoma Street and...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-13

....]; Archeological Resources Protection Act of 1977 (ARPA) [16 U.S.C. 470(aa)-470(ll)]; Archeological and Historic...) [42 U.S.C. 2000(d)-2000(d)(1)]. 7. Wetlands and Water Resources: Clean Water Act (Section 404, Section....O. 11990 Protection of Wetlands; E.O. 11988 Floodplain Management; E.O. 11514 Protection and...
Enhanced angiogenic effect of adipose-derived stromal cell spheroid with low-level light therapy in hindlimb ischemia mice

NASA Astrophysics Data System (ADS)

Park, In-Su; Ahn, Jin-Chul; Chung, Phil-Sang

2014-02-01

Adipose-derived stromal cells (ASCs) are attractive cell source for tissue engineering. However, one obstacle to this approach is that the transplanted ASC population can decline rapidly in the recipient tissue. The aim of this study was to investigate the effects of low-level laser therapy (LLLT) on transplanted human ASCs (hASCs) spheroid in a hindlimb ischemia animal model. LLLT, hASCs spheroid and hASCs spheroid transplantation with LLLT (spheroid + LLLT) were applied to the ischemic hindlimbs in athymic mice. The survival, differentiation and secretion of vascular endothelial growth (VEGF) of spheroid ASCs were evaluated by immunohistochemistry. The spheroid + LLLT group enhanced the tissue regeneration, including angiogenesis, compared with other groups. The spheroid contributed tissue regeneration via differentiation and secretion of growth factors. In the spheroid + LLLT group, the survival of spheroid hASCs was increased by the decreased apoptosis of spheroid hASCs in the ischemic hindlimb. The secretion of growth factors was stimulated in the spheroid + LLLT group compared with the ASCs group and spheroid group. These data suggest that LLLT is an effective biostimulator of spheroid hASCs in tissue regeneration that enhances the survival of ASCs and stimulates the secretion of growth factors in the ischemic hindlimb.
75 FR 15724 - National Register of Historic Places; Weekly Listing of Historic Properties

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-30

...., Washington, DC 20240; in person (by appointment), 1201 Eye St., NW., 8th floor, Washington, DC 20005; by fax... WASHINGTON Pierce County Blue Mouse Theatre, 2611 N. Proctor St., Tacoma, 09001235, LISTED, 1/13/10 (Movie...
Sustained release of adipose-derived stem cells by thermosensitive chitosan/gelatin hydrogel for therapeutic angiogenesis.

PubMed

Cheng, Nai-Chen; Lin, Wei-Jhih; Ling, Thai-Yen; Young, Tai-Horng

2017-03-15

Adipose-derived stem cells (ASCs) secrete several angiogenic growth factors and can be applied to treat ischemic tissue. However, transplantation of dissociated ASCs has frequently resulted in rapid cell death. Therefore, we aimed to develop a thermosensitive chitosan/gelatin hydrogel that is capable of ASC sustained release for therapeutic angiogenesis. By blending gelatin in the chitosan thermosensitive hydrogel, we significantly enhanced the viability of the encapsulated ASCs. During in vitro culturing, the gradual degradation of gelatin led to sustained release of ASCs from the chitosan/gelatin hydrogel. In vitro wound healing assays revealed significantly faster cell migration by co-culturing fibroblasts with ASCs encapsulated in chitosan/gelatin hydrogel compared to pure chitosan hydrogels. Additionally, significantly higher concentrations of vascular endothelial growth factor were found in the supernatant of ASC-encapsulated chitosan/gelatin hydrogels. Co-culturing SVEC4-10 endothelial cells with ASC-encapsulated chitosan/gelatin hydrogels resulted in significantly more tube-like structures, indicating the hydrogel's potential in promoting angiogenesis. Chick embryo chorioallantoic membrane assay and mice wound healing model showed significantly higher capillary density after applying ASC-encapsulated chitosan/gelatin hydrogel. Relative to ASC alone or ASC-encapsulated chitosan hydrogel, more ASCs were also found in the wound tissue on post-wounding day 5 after applying ASC-encapsulated chitosan/gelatin hydrogel. Therefore, chitosan/gelatin thermosensitive hydrogels not only maintain ASC survival, they also enable sustained release of ASCs for therapeutic angiogenesis applications, thereby exhibiting great clinical potential in treating ischemic diseases. Adipose-derived stem cells (ASCs) exhibit great potential to treat ischemic diseases. However, poor delivery methods lead to low cellular survival or dispersal of cells from target sites. In this study, we
Adipose stem cells can secrete angiogenic factors that inhibit hyaline cartilage regeneration.

PubMed

Lee, Christopher Sd; Burnsed, Olivia A; Raghuram, Vineeth; Kalisvaart, Jonathan; Boyan, Barbara D; Schwartz, Zvi

2012-08-24

Adipose stem cells (ASCs) secrete many trophic factors that can stimulate tissue repair, including angiogenic factors, but little is known about how ASCs and their secreted factors influence cartilage regeneration. Therefore, the aim of this study was to determine the effects ASC-secreted factors have in repairing chondral defects. ASCs isolated from male Sprague Dawley rats were cultured in monolayer or alginate microbeads supplemented with growth (GM) or chondrogenic medium (CM). Subsequent co-culture, conditioned media, and in vivo cartilage defect studies were performed. ASC monolayers and microbeads cultured in CM had decreased FGF-2 gene expression and VEGF-A secretion compared to ASCs cultured in GM. Chondrocytes co-cultured with GM-cultured ASCs for 7 days had decreased mRNAs for col2, comp, and runx2. Chondrocytes treated for 12 or 24 hours with conditioned medium from GM-cultured ASCs had reduced sox9, acan, and col2 mRNAs; reduced proliferation and proteoglycan synthesis; and increased apoptosis. ASC-conditioned medium also increased endothelial cell tube lengthening whereas conditioned medium from CM-cultured ASCs had no effect. Treating ASCs with CM reduced or abolished these deleterious effects while adding a neutralizing antibody for VEGF-A eliminated ASC-conditioned medium induced chondrocyte apoptosis and restored proteoglycan synthesis. FGF-2 also mitigated the deleterious effects VEGF-A had on chondrocyte apoptosis and phenotype. When GM-grown ASC pellets were implanted in 1 mm non-critical hyaline cartilage defects in vivo, cartilage regeneration was inhibited as evaluated by radiographic and equilibrium partitioning of an ionic contrast agent via microCT imaging. Histology revealed that defects with GM-cultured ASCs had no tissue ingrowth from the edges of the defect whereas empty defects and defects with CM-grown ASCs had similar amounts of neocartilage formation. ASCs must be treated to reduce the secretion of VEGF-A and other factors that
Myogenic potential of mesenchymal stem cells isolated from porcine adipose tissue.

PubMed

Milner, Derek J; Bionaz, Massimo; Monaco, Elisa; Cameron, Jo Ann; Wheeler, Matthew B

2018-06-01

Advances in stem cell biology and materials science have provided a basis for developing tissue engineering methods to repair muscle injury. Among stem cell populations with potential to aid muscle repair, adipose-derived mesenchymal stem cells (ASC) hold great promise. To evaluate the possibility of using porcine ASC for muscle regeneration studies, we co-cultured porcine ASC with murine C 2 C 12 myoblasts. These experiments demonstrated that porcine ASC display significant myogenic potential. Co-culture of ASC expressing green fluorescent protein (GFP) with C 2 C 12 cells resulted in GFP + myotube formation, indicating fusion of ASC with myoblasts to form myotubes. The presence of porcine lamin A/C positive nuclei in myotubes and RTqPCR analysis of porcine myogenin and desmin expression confirmed that myotube nuclei derived from ASC contribute to muscle gene expression. Co-culturing GFP + ASC with porcine satellite cells demonstrated enhanced myogenic capability of ASC, as the percentage of labeled myotubes increased compared to mouse co-cultures. Enhancing myogenic potential of ASC through soluble factor treatment or expansion of ASC with innate myogenic capacity should allow for their therapeutic use to regenerate muscle tissue lost to disease or injury.
12 CFR 1102.304 - Federal Register publication.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Federal Register publication. 1102.304 Section 1102.304 Banks and Banking FEDERAL FINANCIAL INSTITUTIONS EXAMINATION COUNCIL APPRAISER REGULATION Description of Office, Procedures, Public Information § 1102.304 Federal Register publication. The ASC...
42 CFR 416.40 - Condition for coverage-Compliance with State licensure law.

Code of Federal Regulations, 2010 CFR

2010-10-01

... licensure law. 416.40 Section 416.40 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM AMBULATORY SURGICAL SERVICES Specific Conditions for Coverage § 416.40 Condition for coverage—Compliance with State licensure law. The ASC must comply...
12 CFR 1102.304 - Federal Register publication.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 12 Banks and Banking 9 2013-01-01 2013-01-01 false Federal Register publication. 1102.304 Section 1102.304 Banks and Banking FEDERAL FINANCIAL INSTITUTIONS EXAMINATION COUNCIL APPRAISER REGULATION Description of Office, Procedures, Public Information § 1102.304 Federal Register publication. The ASC...
Fast isolation and expansion of multipotent cells from adipose tissue based on chitosan-selected primary culture.

PubMed

Huang, Guo-Shiang; Tseng, Ting-Chen; Dai, Niann-Tzyy; Fu, Keng-Yen; Dai, Lien-Guo; Hsu, Shan-Hui

2015-10-01

Adipose-derived adult stem cells (ASCs) have gained much attention because of their multipotency and easy access. Here we describe a novel chitosan-based selection (CS) system instead of the conventional plastic adherence (PA) to obtain the primary ASCs. The minimal amount of adipose tissue for consistent isolation of ASCs is reduced from 10 mL to 5 mL. The selection is based on the specific interaction between cells and chitosan materials, which separate ASCs by forming spheroids during primary culture. The primary culture period was reduced from 4 days to one day and more ASCs (ten-fold expansion) were achieved in a week. The average duration for obtaining 1 × 10(7) cells takes about seven days from 5 mL of adipose tissue, compared to 14 days using the conventional PA method from 10 mL of adipose tissue. The replicative senescence of CS-ASCs is not evident until the fifteenth passage (vs. eighth for the PA-ASCs). The obtained ASCs (CS-ASCs) have less doubling time for the same passage of cells and show greater stemness than those obtained from the conventional PA method (PA-ASCs). Moreover, CS-ASCs undergo trilineage differentiation more effectively than PA-ASCs. The greater differentiation potential of CS-ASCs may be associated with the enrichment and maintenance of CD271 positive cells by chitosan selection of primary culture. Copyright © 2015 Elsevier Ltd. All rights reserved.

14 CFR Appendix D to Part 91 - Airports/Locations: Special Operating Restrictions

Code of Federal Regulations, 2010 CFR

2010-01-01

... (Tampa International Airport) Washington, DC (Ronald Reagan Washington National Airport and Andrews Air... Francisco, CA (San Francisco International Airport) Washington, DC (Ronald Reagan Washington National... (Seattle-Tacoma International Airport) Tampa, FL (Tampa International Airport) Washington, DC (Ronald...
14 CFR Appendix D to Part 91 - Airports/Locations: Special Operating Restrictions

Code of Federal Regulations, 2011 CFR

2011-01-01

... (Tampa International Airport) Washington, DC (Ronald Reagan Washington National Airport and Andrews Air... Francisco, CA (San Francisco International Airport) Washington, DC (Ronald Reagan Washington National... (Seattle-Tacoma International Airport) Tampa, FL (Tampa International Airport) Washington, DC (Ronald...
Language Integrated Technology Project Final Evaluation Report.

ERIC Educational Resources Information Center

Stiegemeier, Lois

The goal of the Language Integrated Technology Grant Project (LIT) consortium was to help provide critical components of successful reading programs through a combination of proven computer/print programs and teacher training. Through leadership provided by the Educational Service District 113 (Olympia, Washington), the LIT consortium of schools…
Compartment C1, engine room. View down compionway of top of ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Compartment C-1, engine room. View down compionway of top of engine cylinder heads; note slots in frame at left of photograph, slots hold steel bars which form a protective grating above the engines during battle. (081) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
Specialization and production cost efficiency: evidence from ambulatory surgery centers.

PubMed

Carey, Kathleen; Mitchell, Jean M

2018-03-01

In the U.S. health care sector, the economic logic of specialization as an organizing principle has come under active debate in recent years. An understudied case is that of ambulatory surgery centers (ASCs), which recently have become the dominant provider of specific surgical procedures. While the majority of ASCs focus on a single specialty, a growing number are diversifying to offer a wide range of surgical services. We take a multiple output cost function approach to an empirical investigation that compares production economies in single specialty ASCs with those in multispecialty ASCs. We applied generalized estimating equation techniques to a sample of Pennsylvania ASCs for the period 2004-2014, including 73 ASCs that specialized in gastrointestinal procedures and 60 ASCs that performed gastrointestinal as well as other specialty procedures. Results indicated that both types of ASC had small room for expansion. In simulation analysis, production of GI services in specialized ASCs had a cost advantage over joint production of GI with other specialty procedures. Our results provide support for the focused factory model of production in the ASC sector.
Extracellular matrix and α5β1 integrin signaling control the maintenance of bone formation capacity by human adipose-derived stromal cells

PubMed Central

Di Maggio, Nunzia; Martella, Elisa; Frismantiene, Agne; Resink, Therese J.; Schreiner, Simone; Lucarelli, Enrico; Jaquiery, Claude; Schaefer, Dirk J.; Martin, Ivan; Scherberich, Arnaud

2017-01-01

Stromal vascular fraction (SVF) cells of human adipose tissue have the capacity to generate osteogenic grafts with intrinsic vasculogenic properties. However, adipose-derived stromal/stem cells (ASC), even after minimal monolayer expansion, display poor osteogenic capacity in vivo. We investigated whether ASC bone-forming capacity may be maintained by culture within a self-produced extracellular matrix (ECM) that recapitulates the native environment. SVF cells expanded without passaging up to 28 days (Unpass-ASC) deposited a fibronectin-rich extracellular matrix and displayed greater clonogenicity and differentiation potential in vitro compared to ASC expanded only for 6 days (P0-ASC) or for 28 days with regular passaging (Pass-ASC). When implanted subcutaneously, Unpass-ASC produced bone tissue similarly to SVF cells, in contrast to P0- and Pass-ASC, which mainly formed fibrous tissue. Interestingly, clonogenic progenitors from native SVF and Unpass-ASC expressed low levels of the fibronectin receptor α5 integrin (CD49e), which was instead upregulated in P0- and Pass-ASC. Mechanistically, induced activation of α5β1 integrin in Unpass-ASC led to a significant loss of bone formation in vivo. This study shows that ECM and regulation of α5β1-integrin signaling preserve ASC progenitor properties, including bone tissue-forming capacity, during in vitro expansion. PMID:28290502
Prevention of skin flap necrosis by use of adipose-derived stromal cells with light-emitting diode phototherapy.

PubMed

Park, In-Su; Mondal, Arindam; Chung, Phil-Sang; Ahn, Jin Chul

2015-03-01

The aim of this study was to investigate the effects of low-level light therapy (LLLT) on transplanted human adipose-derived mesenchymal stromal cells (ASCs) in the skin flap of mice. LLLT, ASC transplantation and ASC transplantation with LLLT (ASC + LLLT) were applied to the skin flap. Immunostaining and Western blot analysis were performed to evaluate cell survival and differentiation and secretion of vascular endothelial growth factor and basic fibroblast growth factor by the ASCs. Vascular regeneration was assessed by means of immunostaining in addition to hematoxylin and eosin staining. In the ASC + LLLT group, the survival of ASCs was increased as the result of the decreased apoptosis of ASCs. The secretion of growth factors was higher in this group as compared with ASCs alone. ASCs contributed to tissue regeneration through vascular cell differentiation and secretion of angiogenic growth factors. The ASC + LLLT group displayed improved treatment efficacy including neovascularization and tissue regeneration compared with ASCs alone. Transplanting ASCs to ischemic skin flaps improved therapeutic efficacy for ischemia treatment as the result of enhanced cell survival and paracrine effects. These data suggest that LLLT is an effective biostimulator of ASCs in vascular regeneration, which enhances the survival of ASCs and stimulates the secretion of growth factors in skin flaps. Copyright © 2015 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.
Cielo Computational Environment Usage Model With Mappings to ACE Requirements for the General Availability User Environment Capabilities Release Version 1.1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vigil,Benny Manuel; Ballance, Robert; Haskell, Karen

Cielo is a massively parallel supercomputer funded by the DOE/NNSA Advanced Simulation and Computing (ASC) program, and operated by the Alliance for Computing at Extreme Scale (ACES), a partnership between Los Alamos National Laboratory (LANL) and Sandia National Laboratories (SNL). The primary Cielo compute platform is physically located at Los Alamos National Laboratory. This Cielo Computational Environment Usage Model documents the capabilities and the environment to be provided for the Q1 FY12 Level 2 Cielo Capability Computing (CCC) Platform Production Readiness Milestone. This document describes specific capabilities, tools, and procedures to support both local and remote users. The model ismore » focused on the needs of the ASC user working in the secure computing environments at Lawrence Livermore National Laboratory (LLNL), Los Alamos National Laboratory, or Sandia National Laboratories, but also addresses the needs of users working in the unclassified environment. The Cielo Computational Environment Usage Model maps the provided capabilities to the tri-Lab ASC Computing Environment (ACE) Version 8.0 requirements. The ACE requirements reflect the high performance computing requirements for the Production Readiness Milestone user environment capabilities of the ASC community. A description of ACE requirements met, and those requirements that are not met, are included in each section of this document. The Cielo Computing Environment, along with the ACE mappings, has been issued and reviewed throughout the tri-Lab community.« less
Dyspraxia and autistic traits in adults with and without autism spectrum conditions.

PubMed

Cassidy, Sarah; Hannant, Penelope; Tavassoli, Teresa; Allison, Carrie; Smith, Paula; Baron-Cohen, Simon

2016-01-01

Autism spectrum conditions (ASC) are frequently associated with motor coordination difficulties. However, no studies have explored the prevalence of dyspraxia in a large sample of individuals with and without ASC or associations between dyspraxia and autistic traits in these individuals. Two thousand eight hundred seventy-one adults (with ASC) and 10,706 controls (without ASC) self-reported whether they have been diagnosed with dyspraxia. A subsample of participants then completed the Autism Spectrum Quotient (AQ; 1237 ASC and 6765 controls) and the Empathy Quotient (EQ; 1147 ASC and 6129 controls) online through the Autism Research Centre website. The prevalence of dyspraxia was compared between those with and without ASC. AQ and EQ scores were compared across the four groups: (1) adults with ASC with dyspraxia, (2) adults with ASC without dyspraxia, (3) controls with dyspraxia, and (4) controls without dyspraxia. Adults with ASC were significantly more likely to report a diagnosis of dyspraxia (6.9%) than those without ASC (0.8%). In the ASC group, those with co-morbid diagnosis of dyspraxia did not have significantly different AQ or EQ scores than those without co-morbid dyspraxia. However, in the control group (without ASC), those with dyspraxia had significantly higher AQ and lower EQ scores than those without dyspraxia. Dyspraxia is significantly more prevalent in adults with ASC compared to controls, confirming reports that motor coordination difficulties are significantly more common in this group. Interestingly, in the general population, dyspraxia was associated with significantly higher autistic traits and lower empathy. These results suggest that motor coordination skills are important for effective social skills and empathy.
Ascorbyl Stearate Promotes Apoptosis Through Intrinsic Mitochondrial Pathway in HeLa Cancer Cells.

PubMed

Mane, Shirish D; Thoh, Maikho; Sharma, Deepak; Sandur, Santosh K; Naidu, K Akhilender

2016-12-01

Ascorbic acid is proposed to have antitumor potential against certain cancer types but has the limitation of requiring high doses for treating cancer. Ascorbyl stearate (ASC-S) is a fatty acid ester derivative of ascorbic acid with comparable potent apoptotic activity. The present study was aimed at understanding the pathway involved in apoptotic activity of ASC-S in cervical cancer cells. The effect of ASC-S on reactive oxygen species (ROS), and mitochondrial membrane potential (MMP) was studied in HeLa cells. Furthermore, the dose-dependent effect of ASC-S on release of cytochrome c, pro-caspase-9, caspase-3, BH3 interacting-domain death agonist (BID), truncated BH3 interacting-domain death agonist (t-BID), FAS ligand (FASL) and transcription factors nuclear factor-kappa B (NF-ĸB), nuclear factor of activated T-cells (NFAT) and activator protein-1 (AP1) were studied in HeLa cells. Treatment of HeLa cells with ASC-S significantly increased the MMP. The modulation of MMP resulted in cleavage of BID, expression of FAS, cleavage of pro-caspase-9 and release of cytochrome c into cytosol. In addition, ASC-S treatment resulted in deregulation of transcription factors NF-ĸB, NFAT and AP1, which play an important role in the development of inflammation and cancer. Our data, for the first time, suggest that ASC-S has an apoptotic effect against HeLa cells by inducing change in mitochondrial membrane permeability, cytochrome c release and subsequent activation of caspase-3 and NF-ĸB. Copyright© 2016 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
Why a Trash-Strewn Lot Became a Soccer Field.

PubMed

Romano, Michael

2016-07-01

Five years ago, a coalition of community groups, government agencies and nonprofit organizations was struggling to reduce violent behavior and delinquency among under- privileged youths in a low-income suburban neighborhood about 15 miles north of Tacoma, Washington.
Adipose-Derived Stem Cells for Tissue Engineering and Regenerative Medicine Applications

PubMed Central

Dai, Ru; Wang, Zongjie; Samanipour, Roya; Koo, Kyo-in; Kim, Keekyoung

2016-01-01

Adipose-derived stem cells (ASCs) are a mesenchymal stem cell source with properties of self-renewal and multipotential differentiation. Compared to bone marrow-derived stem cells (BMSCs), ASCs can be derived from more sources and are harvested more easily. Three-dimensional (3D) tissue engineering scaffolds are better able to mimic the in vivo cellular microenvironment, which benefits the localization, attachment, proliferation, and differentiation of ASCs. Therefore, tissue-engineered ASCs are recognized as an attractive substitute for tissue and organ transplantation. In this paper, we review the characteristics of ASCs, as well as the biomaterials and tissue engineering methods used to proliferate and differentiate ASCs in a 3D environment. Clinical applications of tissue-engineered ASCs are also discussed to reveal the potential and feasibility of using tissue-engineered ASCs in regenerative medicine. PMID:27057174
Progress made in understanding Mount Rainier's hazards

USGS Publications Warehouse

Sisson, T.W.; Vallance, J.W.; Pringle, P.T.

2001-01-01

At 4392 m high, glacier-clad Mount Rainier dominates the skyline of the southern Puget Sound region and is the centerpiece of Mount Rainier National Park. About 2.5 million people of the greater Seattle-Tacoma metropolitan area can see Mount Rainier on clear days, and 150,000 live in areas swept by lahars and floods that emanated from the volcano during the last 6,000 years (Figure 1). These lahars include the voluminous Osceola Mudflow that floors the lowlands south of Seattle and east of Tacoma, and which was generated by massive volcano flank-collapse. Mount Rainier's last eruption was a light dusting of ash in 1894; minor pumice last erupted between 1820 and 1854; and the most recent large eruptions we know of were about 1100 and 2300 years ago, according to reports from the U.S. Geological Survey.
Teaching the Physics of Energy While Traveling by Train

NASA Astrophysics Data System (ADS)

Hay, Katrina

2013-02-01

Pacific Lutheran University (Tacoma, WA) is renowned for the number of its courses that offer international and study-away opportunities. Inspired by the theme of sustainability, and my growing concern about the environmental impact of conventional fuels, I offered a course, Physics of Energy, for the first time during PLU's January 2011 term (a one-month semester). The two-week travel portion of the course took students on the Amtrak Coast Starlight train route from Tacoma, WA, to Los Angeles to study various forms of energy production. Students studied physics topics in the classroom, through hands-on activities, by traveling to energy research and production facilities, and while working on their interdisciplinary term projects. The course, which utilized local talent and focused on sustainability, provided a positive physics learning experience for majors and non-majors alike.
Enhanced angiogenic effect of adipose-derived stromal cell spheroid with low-level light therapy in hind limb ischemia mice.

PubMed

Park, In-Su; Chung, Phil-Sang; Ahn, Jin Chul

2014-11-01

The aim of this study was to investigate the effects of low-level laser therapy (LLLT) on transplanted human adipose-derived mesenchymal stem cells (hASCs) spheroid in a hind limb ischemia animal model. LLLT, hASCs spheroid and hASCs spheroid transplantation with LLLT (spheroid + LLLT) were applied to the ischemic hind limbs in athymic mice. The survival, differentiation and secretion of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGF), and hepatocyte growth factor (HGF) of the spheroid ASCs were evaluated by immunohistochemistry and western blots. Spheroid + LLLT group had enhanced the tissue regeneration, including angiogenesis, compared with the ASC group. The spheroid ASCs contributed to tissue regeneration via differentiation and secretion of growth factors. In the spheroid + LLLT group, the survival of spheroid hASCs increased with a concomitant decrease in apoptosis of spheroid hASCs in the ischemic hind limb. The secretion of growth factors was stimulated in the spheroid + LLLT group compared with the ASCs and spheroid group. These data suggested that LLLT is an effective biostimulator of spheroid hASCs in tissue regeneration that enhanced the survival of ASCs and stimulated the secretion of growth factors in the ischemic hind limb. Copyright © 2014 Elsevier Ltd. All rights reserved.
Progranulin, a Major Secreted Protein of Mouse Adipose-Derived Stem Cells, Inhibits Light-Induced Retinal Degeneration

PubMed Central

Tsuruma, Kazuhiro; Yamauchi, Mika; Sugitani, Sou; Otsuka, Tomohiro; Ohno, Yuta; Nagahara, Yuki; Ikegame, Yuka; Shimazawa, Masamitsu; Yoshimura, Shinichi; Iwama, Toru

2014-01-01

Adipose tissue stromal vascular fraction contains mesenchymal stem cells, which show protective effects when administered to damaged tissues, mainly through secreted trophic factors. We examined the protective effects of adipose-derived stem cells (ASCs) and ASC-conditioned medium (ASC-CM) against retinal damage and identified the neuroprotective factors in ASC-CM. ASCs and mature adipocytes were isolated from mouse subcutaneous tissue. ASCs were injected intravitreally in a mouse model of light-induced retinal damage, and ASC injection recovered retinal function as measured by electroretinogram and inhibited outer nuclear layer, thinning, without engraftment of ASCs. ASC-CM and mature adipocyte-conditioned medium were collected after 72 hours of culture. In vitro, H2O2- and light-induced cell death was reduced in a photoreceptor cell line with ASC-CM but not with mature adipocyte-conditioned medium. In vivo, light-induced photoreceptor damage was evaluated by measurement of outer nuclear layer thickness at 5 days after light exposure and by electroretinogram recording. ASC-CM significantly inhibited photoreceptor degeneration and retinal dysfunction after light exposure. Progranulin was identified as a major secreted protein of ASCs that showed protective effects against retinal damage in vitro and in vivo. Furthermore, progranulin phosphorylated extracellular signal-regulated kinase, cAMP response element binding protein, and hepatocyte growth factor receptor, and protein kinase C signaling pathways were involved in the protective effects of progranulin. These findings suggest that ASC-CM and progranulin have neuroprotective effects in the light-induced retinal-damage model. Progranulin may be a potential target for the treatment of the degenerative diseases of the retina. PMID:24233842
Progranulin, a major secreted protein of mouse adipose-derived stem cells, inhibits light-induced retinal degeneration.

PubMed

Tsuruma, Kazuhiro; Yamauchi, Mika; Sugitani, Sou; Otsuka, Tomohiro; Ohno, Yuta; Nagahara, Yuki; Ikegame, Yuka; Shimazawa, Masamitsu; Yoshimura, Shinichi; Iwama, Toru; Hara, Hideaki

2014-01-01

Adipose tissue stromal vascular fraction contains mesenchymal stem cells, which show protective effects when administered to damaged tissues, mainly through secreted trophic factors. We examined the protective effects of adipose-derived stem cells (ASCs) and ASC-conditioned medium (ASC-CM) against retinal damage and identified the neuroprotective factors in ASC-CM. ASCs and mature adipocytes were isolated from mouse subcutaneous tissue. ASCs were injected intravitreally in a mouse model of light-induced retinal damage, and ASC injection recovered retinal function as measured by electroretinogram and inhibited outer nuclear layer, thinning, without engraftment of ASCs. ASC-CM and mature adipocyte-conditioned medium were collected after 72 hours of culture. In vitro, H2O2- and light-induced cell death was reduced in a photoreceptor cell line with ASC-CM but not with mature adipocyte-conditioned medium. In vivo, light-induced photoreceptor damage was evaluated by measurement of outer nuclear layer thickness at 5 days after light exposure and by electroretinogram recording. ASC-CM significantly inhibited photoreceptor degeneration and retinal dysfunction after light exposure. Progranulin was identified as a major secreted protein of ASCs that showed protective effects against retinal damage in vitro and in vivo. Furthermore, progranulin phosphorylated extracellular signal-regulated kinase, cAMP response element binding protein, and hepatocyte growth factor receptor, and protein kinase C signaling pathways were involved in the protective effects of progranulin. These findings suggest that ASC-CM and progranulin have neuroprotective effects in the light-induced retinal-damage model. Progranulin may be a potential target for the treatment of the degenerative diseases of the retina.
42 CFR 416.163 - General rules.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 3 2010-10-01 2010-10-01 false General rules. 416.163 Section 416.163 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... beneficiaries by a participating ASC in connection with covered surgical procedures as determined by the...
42 CFR 416.163 - General rules.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 3 2011-10-01 2011-10-01 false General rules. 416.163 Section 416.163 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... beneficiaries by a participating ASC in connection with covered surgical procedures as determined by the...
42 CFR 416.44 - Condition for coverage-Environment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 3 2011-10-01 2011-10-01 false Condition for coverage-Environment. 416.44 Section... for coverage—Environment. The ASC must have a safe and sanitary environment, properly constructed, equipped, and maintained to protect the health and safety of patients. (a) Standard: Physical environment...

42 CFR 416.44 - Condition for coverage-Environment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 3 2010-10-01 2010-10-01 false Condition for coverage-Environment. 416.44 Section... for coverage—Environment. The ASC must have a safe and sanitary environment, properly constructed, equipped, and maintained to protect the health and safety of patients. (a) Standard: Physical environment...
Ambulatory surgery centers--current legal issues 2004 (Part 2).

PubMed

Becker, Scott; Szabad, Melissa; Foltushansky, Sofia

2004-05-01

component of the procedure performed with the equipment, and the vendor then pays the ASC a fee for its lease of ASC space to house the equipment. These types of relationships can raise several legal compliance concerns. An ASC should understand that the arrangement could jeopardize its state ASC license and its Medicare certification even though the arrangement does not involve Medicare certified procedures or Medicare patients. Furthermore, this type of business venture might not comply with state and federal self referral laws. ASCs are also increasingly facing situations where a provider performs a procedure for which there is no facility charge for the ASC, but the physician is receiving a professional fee from Medicare. In this situation, ASCs should implement a policy, similar to the example provided in this article, to avoid even the appearance of providing an inducement or reward for referrals of Medicare or Medicaid business by requiring physicians to pay the Center a fee, consistent with fair market value, for the use of the Center for non-Covered Procedures or for performing multiple procedures within the same operative session. Lastly, as touched upon by this article, in the joint venture context, ASCs must take into account several guidelines in order to steer clear from risks associated with tax-exempt entities as an investor and antitrust price fixing issues. Whether a joint venture, of which a tax exempt entity is an investor, is deemed organized and operated in furtherance of the charitable purposes of a tax-exempt partner as set forth in Section 501(c)(3) of the Code depends on the facts and circumstances of each case. Generally, a joint venture will satisfy the guidelines if the tax-exempt investor in the joint venture retains sufficient control over the joint venture to ensure that the joint venture furthers the charitable purposes of the tax-exempt investor and adequately serves the community. In the ASC physician hospital joint venture arena, the
33 CFR 117.1061 - Tacoma Harbor.

Code of Federal Regulations, 2011 CFR

2011-07-01

... at least two hours notice is given. However the draw need not be opened from 6:30 a.m. to 8:30 a.m. and 3:30 p.m. to 5:30 p.m. Monday through Friday, except Federal holidays for vessels of less than 1... emergencies, openings shall be made as soon as possible upon notification to the Washington State Department...
5. Photocopy of a 1943 architectural drawing titled: 'Patients & ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Photocopy of a 1943 architectural drawing titled: 'Patients & Detachment Mess, MESS-Z-H. Floor Plan, Part A.' 1-13-43 - Madigan Hospital, Patients' & Medical Detachments, Bounded by Wilson & McKinley Avenues & Garfield & Lincoln Streets, Tacoma, Pierce County, WA
6. Photocopy of a 1943 architectural drawing titled: 'Patients & ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. Photocopy of a 1943 architectural drawing titled: 'Patients & Detachment Mess, MESS-Z-H. Floor Plan, Part B.' 1-13-43 - Madigan Hospital, Patients' & Medical Detachments, Bounded by Wilson & McKinley Avenues & Garfield & Lincoln Streets, Tacoma, Pierce County, WA
5. Corridor A and Building No. 9962A (with white door). ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. Corridor A and Building No. 9962-A (with white door). In upper left is east side of Building No. 9952-B. - Madigan Hospital, Corridors & Ramps, Bounded by Wilson & McKinley Avenues & Garfield & Lincoln Streets, Tacoma, Pierce County, WA
Trinidad Lake asphalt overlay performance : final report.

DOT National Transportation Integrated Search

2014-07-01

Construction of the new Tacoma Narrows Bridge (TNB) included a steel orthotropic bridge deck. : The higher flexibility of an orthotropic deck causes pavement placed upon it to fatigue and crack more : quickly than pavement placed on a normal roadway....
Introduction

Treesearch

Robert O. Curtis; David D. Marshall; Dean S. DeBell

2004-01-01

Silviculturists of the Olympia Forestry Sciences Laboratory, Pacific Northwest Research Station, have joined with foresters of the Washington State Department of Natural Resources and scientists of University of Washington and University of Idaho to establish a long-term comparison of silvicultural regimes for regeneration and management of young growth Douglas-fir (...
Normal yield tables for red alder.

Treesearch

Norman P. Worthington; Floyd A. Johnson; George R. Staebler; William J. Lloyd

1960-01-01

Increasing interest in the management of red alder (Alnus rubra) has created a need for reliable yield information. Existing yield tables for red alder have been very useful as interim sources of information, but they are generally inadequate for current and prospective management needs. The advisory committee for the Station's Olympia...
75 FR 31413 - Notice of Funding Availability (NOFA) Inviting Applications for the Rural Microentrepreneur...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-03

... Rural Development State Office, 1835 Black Lake Boulevard SW., Suite B, Olympia, WA 98512-5715, (360... TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination write to USDA... or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider...
Alcohol and Staff Leisure Time.

ERIC Educational Resources Information Center

Camping Magazine, 1992

1992-01-01

Discusses the problem of alcohol use and abuse by camp staff. Describes alcohol policies of two different camps. Camp Highlands allows responsible drinking but not intoxication. Camp Olympia requires total abstinence from alcohol. A policy that clearly expresses the camp's philosophy toward alcohol and spells out all expectations and results is…
40 CFR 60.4 - Address.

Code of Federal Regulations, 2011 CFR

2011-07-01

... Department of Pollution Control and Ecology, 8001 National Drive, P.O. Box 9583, Little Rock, AR 72209. (F... State Department of Ecology (Ecology), P.O. Box 47600, Olympia, WA 98504-7600, http://www.ecy.wa.gov... not delegated. NSPS Subparts Delegated to Washington Air Agencies Subpart 1 Washington Ecology 2 BCAA...
77 FR 53168 - Olympic Peninsula Resource Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-31

.... The purpose of the committee is to improve collaborative relationships and to provide advice and.../advisorycommittees?cid=fsbdev3_049547 . Anyone who would like to bring related matters to the attention of the... Blvd. SW., Olympia, WA 98512, attention Grace Haight, or by email to [email protected] or via...
Follow-Up to the 2002 Futures Conference: Collaborating to Serve All Children, Families, and Schools

ERIC Educational Resources Information Center

Cummings, Jack A.; Harrison, Patti L.; Dawson, Margaret M.; Short, Rick J.; Gorin, Susan; Palomares, Ronald S.

2004-01-01

Recommendations of the Thayer, Spring Hill, and Olympia conferences are examined as they relate to ways we can support and make positive contributions to students, families, and schools. In the current issue of the Journal of Educational and Psychological Consultation, Hatzichristou and Lampropoulou (this issue), Meyers, Meyers, and Grogg (this…
Ultraviolet B preconditioning enhances the hair growth-promoting effects of adipose-derived stem cells via generation of reactive oxygen species.

PubMed

Jeong, Yun-Mi; Sung, Young Kwan; Kim, Wang-Kyun; Kim, Ji Hye; Kwack, Mi Hee; Yoon, Insoo; Kim, Dae-Duk; Sung, Jong-Hyuk

2013-01-01

Hypoxia induces the survival and regenerative potential of adipose-derived stem cells (ASCs), but there are tremendous needs to find alternative methods for ASC preconditioning. Therefore, this work investigated: (1) the ability of low-dose ultraviolet B (UVB) radiation to stimulate the survival, migration, and tube-forming activity of ASCs in vitro; (2) the ability of UVB preconditioning to enhance the hair growth-promoting capacity of ASCs in vivo; and (3) the mechanism of action for ASC stimulation by UVB. Although high-dose UVB decreased the proliferation of ASCs, low-dose (10 or 20 mJ/cm(2)) treatment increased their survival, migration, and tube-forming activity. In addition, low-dose UVB upregulated the expression of ASC-derived growth factors, and a culture medium conditioned by UVB-irradiated ASCs increased the proliferation of dermal papilla and outer root sheet cells. Notably, injection of UVB-preconditioned ASCs into C(3)H/HeN mice significantly induced the telogen-to-anagen transition and increased new hair weight in vivo. UVB treatment significantly increased the generation of reactive oxygen species (ROS) in cultured ASCs, and inhibition of ROS generation by diphenyleneiodonium chloride (DPI) significantly attenuated UVB-induced ASC stimulation. Furthermore, NADPH oxidase 4 (Nox4) expression was induced in ASCs by UVB irradiation, and Nox4 silencing by small interfering RNA, like DPI, significantly reduced UVB-induced ROS generation. These results suggest that the primary involvement of ROS generation in UVB-mediated ASC stimulation occurs via the Nox4 enzyme. This is the first indication that a low dose of UVB radiation and/or the control of ROS generation could potentially be incorporated into a novel ASC preconditioning method for hair regeneration.
The morphological regeneration and functional restoration of bladder defects by a novel scaffold and adipose-derived stem cells in a rat augmentation model.

PubMed

Wang, Qiong; Xiao, Dong-Dong; Yan, Hao; Zhao, Yang; Fu, Shi; Zhou, Juan; Wang, Zhong; Zhou, Zhe; Zhang, Ming; Lu, Mu-Jun

2017-06-24

Due to the multilineage differentiation ability and paracrine role of adipose-derived stem cells (ASCs) for bladder defect repair, various scaffolds have been applied in combination with ASCs to promote bladder regeneration and restore bladder function. However, the low survival rate of ASCs and the difficulty of promoting bladder functional recovery are still unsolved. To explore these problems, we investigated the feasibility of a novel scaffold seeded with ASCs in a rat model of bladder augmentation. A novel autologous myofibroblast (AM)-silk fibroin (SF) scaffold was harvested after subcutaneously prefabricating the bladder acellular matrix grafts (BAMG) and SF by removing the BAMG. The AM-SF scaffolds were then seeded with ASCs (AM-SF-ASCs). Fifty percent supratrigonal cystectomies were performed followed by augmenting the cystectomized defects with AM-SF scaffolds or AM-SF-ASCs. The histological and functional assessments of bladders were performed 2, 4, and 12 weeks after surgery while the ASCs were tracked in vivo. For bladder tissue regeneration, immunofluorescence analysis revealed that AM-SF-ASCs (the experimental group) promoted better morphological regeneration of the urothelium, vessels, bladder smooth muscle, and nerve than AM-SF scaffolds (the control group). Regarding functional restoration, the AM-SF-ASC group exhibited higher bladder compliance and relatively normal micturition pattern compared to the AM-SF group. In addition, a certain number of surviving ASCs could be found in vivo 12 weeks after implantation, and some of them had differentiated into smooth muscle cells. The AM-SF scaffolds with ASCs could rapidly promote bladder morphological regeneration and improved bladder urinary function. In addition, the bag-shaped structure of the AM-SF scaffold can improve the survival of ASCs for at least 12 weeks. This strategy of AM-SF-ASCs has a potential to repair large-scale bladder defects in the clinic in the future.
The molecular mechanism underlying the proliferating and preconditioning effect of vitamin C on adipose-derived stem cells.

PubMed

Kim, Ji Hye; Kim, Wang-Kyun; Sung, Young Kwan; Kwack, Mi Hee; Song, Seung Yong; Choi, Joon-Seok; Park, Sang Gyu; Yi, TacGhee; Lee, Hyun-Joo; Kim, Dae-Duk; Seo, Hyun Min; Song, Sun U; Sung, Jong-Hyuk

2014-06-15

Although adipose-derived stem cells (ASCs) show promise for cell therapy, there is a tremendous need for developing ASC activators. In the present study, we investigated whether or not vitamin C increases the survival, proliferation, and hair-regenerative potential of ASCs. In addition, we tried to find the molecular mechanisms underlying the vitamin C-mediated stimulation of ASCs. Sodium-dependent vitamin C transporter 2 (SVCT2) is expressed in ASCs, and mediates uptake of vitamin C into ASCs. Vitamin C increased the survival and proliferation of ASCs in a dose-dependent manner. Vitamin C increased ERK1/2 phosphorylation, and inhibition of the mitogen-activated protein kinase (MAPK) pathway attenuated the proliferation of ASCs. Microarray and quantitative polymerase chain reaction showed that vitamin C primarily upregulated expression of proliferation-related genes, including Fos, E2F2, Ier2, Mybl1, Cdc45, JunB, FosB, and Cdca5, whereas Fos knock-down using siRNA significantly decreased vitamin C-mediated ASC proliferation. In addition, vitamin C-treated ASCs accelerated the telogen-to-anagen transition in C3H/HeN mice, and conditioned medium from vitamin C-treated ASCs increased the hair length and the Ki67-positive matrix keratinocytes in hair organ culture. Vitamin C increased the mRNA expression of HGF, IGFBP6, VEGF, bFGF, and KGF, which may mediate hair growth promotion. In summary, vitamin C is transported via SVCT2, and increased ASC proliferation is mediated by the MAPK pathway. In addition, vitamin C preconditioning enhanced the hair growth promoting effect of ASCs. Because vitamin C is safe and effective, it could be used to increase the yield and regenerative potential of ASCs.
Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2.

PubMed

Kim, Jong-Ho; Lim, I-Rang; Joo, Hyung Joon; Choi, Seung-Cheol; Choi, Ji-Hyun; Cui, Long-Hui; Im, Lisa; Hong, Soon Jun; Lim, Do-Sun

A number of researchers have been reporting a wide range of in vitro and in vivo studies of cell engraftment to enhance angiogenesis using stem cells. Despite these efforts, studies involving three-dimensional (3D) culture method that mimics in vivo environment have not reached its peak yet. In this study, we investigated the change and effects on cellular angiogenic growth factors through sphere formation of adipose stem cell (ASC) which is engineered by poly-2-hydroxyethyl methacrylate (Poly-HEMA). First of all, we successfully induced sphere formation of ASC (sph-ASC) on Poly-HEMA coated plates. sph-ASC represented significantly higher expression levels of anti-apoptotic and hypoxic factors compared to monolayer adherent ASC (adh-ASC). Interestingly, sph-ASC showed higher mRNA levels of the following genes; CD31, CD144, vWF, IGF-2, MCP-1, PDGF-A, VEGF-A, VEGF-C, and FGF-2. In addition, mRNA expressions of angiogenic growth factor receptors such as Flk1, FGFR1, FGFR2, and Tie2 were elevated in sph-ASC. In protein level, Cytokine/Chemokines antibody array revealed a significant increase of FGF-2 in sph-ASC (3.17-fold) compared to adh-ASC. To investigate the effects of FGF-2 on sph-ASC, Matrigel angiogenic invasion assay showed significant reduced level of FGF-2 in FGF-2 siRNA transfected sph-ASC (2.27-fold) compared to negative control siRNA transfected sph-ASC. These findings suggest that Poly-HEMA coated plates induce sphere formation of ASC which has significantly higher expression of FGF-2, and plays a critical role as a major regulating growth factor of in vitro angiogenesis. Copyright © 2015 Elsevier Inc. All rights reserved.
7. Photocopy of photographca. 1927 (2 1/4 X 2 1/4' ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Photocopy of photograph--ca. 1927 (2 1/4 X 2 1/4' negative) DETAIL SHOWING ADAPTATION THAT ALLOWED USE OF UPPER END OF ORIGINAL FLUME AND LOWER END JUST RECONSTRUCTED - Power Flume No. 1, Tacoma, La Plata County, CO
1. 133842 PACIFIC AVE. ITALIANATE BUILDING ON LEFT IS THE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. 1338-42 PACIFIC AVE. ITALIANATE BUILDING ON LEFT IS THE CITIZENS BANK AND THE IRVING BUILDING (1888-89). A HOMOGENEOUS DESIGN FOR THREE SEPERATE PROPERTY OWNERS, DESIGNED BY CARL AUGUST DARMER. - Union Depot Area Study, Tacoma, Pierce County, WA

Defense.gov Special Report: U.S. Military Responds in Wake of Earthquake

Science.gov Websites

held the rank of airman basic, was lost on the streets of Tokyo. Story U.S. Forces Safe After New Japan transitioned through Seattle-Tacoma International Airport to safe-haven locations in the United States in March
Banned Books: A Study of Censorship.

ERIC Educational Resources Information Center

Rossuck, Jennifer

1997-01-01

Describes how a course on censorship taught at an all-girls high school in Tacoma, Washington, drew on current event controversies to initiate discussion. Outlines the course's four units and uses Ray Bradbury's "Fahrenheit 451" to frame course questions. (TB)
Extended Operation of Stirling Convertors at NASA Glenn Research Center

NASA Technical Reports Server (NTRS)

Oriti, Salvatore, M.

2012-01-01

NASA Glenn Research Center (GRC) has been supporting development of free-piston Stirling conversion technology for spaceflight electrical power generation since 1999. GRC has also been supporting the development of the Advanced Stirling Radioisotope Generator (ASRG) since 2006. A key element of the ASRG project is providing life, reliability, and performance data for the Advanced Stirling Convertor (ASC). The Thermal Energy Conversion branch at GRC is conducting extended operation of several free-piston Stirling convertors. The goal of this effort is to generate long-term performance data (tens of thousands of hours) on multiple units to build a life and reliability database. Currently, GRC is operating 18 convertors. This hardware set includes Technology Demonstration Convertors (TDCs) from Infinia Corporation, of which one pair (TDCs #13 and #14) has accumulated over 60,000 hr (6.8 years) of operation. Also under test are various Sunpower, Inc. convertors that were fabricated during the ASC development activity, including ASC-0, ASC-E (including those in the ASRG engineering unit), and ASC-E2. The ASC-E2s also completed, or are in progress of completing workmanship vibration testing, performance mapping, and extended operation. Two ASC-E2 units will also be used for durability testing, during which components will be stressed to levels above nominal mission usage. Extended operation data analyses from these tests are covered in this paper.
Differential Regulation of the Ascorbic Acid Transporter SVCT2 during Development and in Response to Ascorbic Acid Depletion

PubMed Central

Meredith, M. Elizabeth; Harrison, Fiona E.; May, James M.

2011-01-01

The sodium-dependent vitamin C transporter-2 (SVCT2) is the only ascorbic acid (ASC) transporter significantly expressed in brain. It is required for life and critical during brain development to supply adequate levels of ASC. To assess SVCT2 function in the developing brain, we studied time-dependent SVCT2 mRNA and protein expression in mouse brain, using liver as a comparison tissue because it is the site of ASC synthesis. We found that SVCT2 expression followed an inverse relationship with ASC levels in the developing brain. In cortex and cerebellum, ASC levels were high throughout late embryonic stages and early post-natal stages and decreased with age, whereas SVCT2 mRNA and protein levels were low in embryos and increased with age. A different response was observed for liver, in which ASC levels and SVCT2 expression were both low throughout embryogenesis and increased post-natally. To determine whether low intracellular ASC might be capable of driving SVCT2 expression, we depleted ASC by diet in adult mice unable to synthesize ASC. We observed that SVCT2 mRNA and protein were not affected by ASC depletion in brain cortex, but SVCT2 protein expression was increased by ASC depletion in the cerebellum and liver. The results suggest that expression of the SVCT2 is differentially regulated during embryonic development and in adulthood. PMID:22001929
42 CFR 416.200 - Payment adjustment.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 3 2010-10-01 2010-10-01 false Payment adjustment. 416.200 Section 416.200 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... in connection with ASC facility services. (b) CMS adjusts the payment for insertion of an IOL...
42 CFR 416.200 - Payment adjustment.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 3 2011-10-01 2011-10-01 false Payment adjustment. 416.200 Section 416.200 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED... in connection with ASC facility services. (b) CMS adjusts the payment for insertion of an IOL...
12 CFR 1102.306 - Procedures for requesting records.

Code of Federal Regulations, 2013 CFR

2013-01-01

... the requirements of paragraph (a) of this section, a search shall be conducted of records maintained... the ASC shall be charged for the direct costs of search, duplication, and review as set forth in... the requester's remittance. (ii) Requesters will be charged for search and review costs even if...
12 CFR 1102.306 - Procedures for requesting records.

Code of Federal Regulations, 2014 CFR

2014-01-01

... the requirements of paragraph (a) of this section, a search shall be conducted of records maintained... the ASC shall be charged for the direct costs of search, duplication, and review as set forth in... the requester's remittance. (ii) Requesters will be charged for search and review costs even if...
12 CFR 1102.306 - Procedures for requesting records.

Code of Federal Regulations, 2012 CFR

2012-01-01

... the requirements of paragraph (a) of this section, a search shall be conducted of records maintained... the ASC shall be charged for the direct costs of search, duplication, and review as set forth in... the requester's remittance. (ii) Requesters will be charged for search and review costs even if...
12 CFR 1102.301 - Definitions.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 7 2010-01-01 2010-01-01 false Definitions. 1102.301 Section 1102.301 Banks... Office, Procedures, Public Information § 1102.301 Definitions. For purposes of this subpart: (a) ASC... disk). (f) Educational institution means a preschool, a public or private elementary or secondary...
Fabrication and evaluation of thermosensitive chitosan/collagen/α, β-glycerophosphate hydrogels for tissue regeneration.

PubMed

Dang, Qifeng; Liu, Kai; Zhang, Zhenzhen; Liu, Chengsheng; Liu, Xi; Xin, Ying; Cheng, Xiaoyu; Xu, Tao; Cha, Dongsu; Fan, Bing

2017-07-01

Thermosensitive hydrogels whose physiological properties are similar to extracellular matrix have been extensively used for tissue regeneration. Polysaccharides and proteins, as biocompatible substrates similar to bio-macromolecules that could be recognized by human body, are two preferred polymers for fabrication of such hydrogels. A series of novel thermosensitive hydrogels (CS-ASC-HGs) containing chitosan (CS) and acid-soluble collagen (ASC) were thus prepared, in the presence of α, β-glycerophosphate, to mimic extracellular microenvironment for tissue regeneration. Rheological measurements demonstrated excellent thermosensitivity. FT-IR and SEM indicated CS-ASC-HGs possessed 3D porous architectures with fibrous ASC, and the molecular structure of ASC was well-maintained in hydrogels. Hemolysis, acute toxicity, and cytotoxicity tests suggested CS-ASC-HGs were of good biocompatibility. CS-ASC-HGs were able to support the survival and proliferation of L929 cells encapsulated in them. Moreover, CS-ASC-HGs had better pH stability and biocompatibility than pure CS hydrogel. These results suggested that CS-ASC-HGs could serve as promising scaffolds for tissue regeneration. Copyright © 2017 Elsevier Ltd. All rights reserved.
Student Interest Inventory -- Opinions of Students.

ERIC Educational Resources Information Center

Olympia Community Unit District 16, Minier, IL.

By virtue of a Title III Elementary and Secondary Education Act grant, a student interest inventory instrument was devised to evaluate apprehensions of students attending 5 different high schools prior to consolidation of the Olympia Community Unit District 16 in Illinois. Initially administered to 183 ninth graders, 228 tenth graders, and 185…
75 FR 21584 - Notice of Solicitation of Applications (NOSA) for Inviting Applications for Renewable Energy...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-26

... Black Lake Blvd. SW., Suite B, Olympia, WA 98512, (360) 704-7762, [email protected] West Virginia... USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to... call (800) 795-3272 (voice), or (202) 720-6382 (TDD). ``USDA is an equal opportunity provider, employer...
76 FR 20943 - Notice of Funds Availability (NOFA) Inviting Applications for the Rural Energy for America Program

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-14

... Florida) Washington Mary Traxler, USDA Rural Development, 1835 Black Lake Blvd. SW., Suite B, Olympia, WA... USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination write to... 20250-9410 or call (800) 795-3272 (voice) or (202) 720- 6382 (TDD). USDA is an equal opportunity...
Culture Shock: A Teacher's Guide To Accompany the Four-Part PBS Series Premiering January 2000.

ERIC Educational Resources Information Center

Jaffee, Cyrisse, Ed.; Sharma, Amina, Ed.

This teacher's guide accompanies the four videos ("Born to Trouble: Adventures of Huckleberry Finn"; "The Shock of the Nude: Manet's Olympia"; "Hollywood Censored: Movies, Morality and the Production Code"; and "The Devil's Music: 1920s Jazz") of the PBS "Culture Shock" series. The guide suggests that the videos could be used in the…
Oklahoma: A View of the Center

ERIC Educational Resources Information Center

Jones, Ruthe Blalock; Depriest, Maria; Fowler, Cynthia

2007-01-01

This article presents a dialogue on twentieth-century Oklahoma artists and writers given at a conference titled "Working from Community: American Indian Art and Literature in a Historical and Cultural Context" and held in the summer of 2003 at Evergreen State College in Olympia, Washington. Twenty-five educators converged for six weeks…
Compartment A126 port side. Note ash hoist for boiler room ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Compartment A-126 port side. Note ash hoist for boiler room compartment B-1 and compartment B-2. Scuttlebutt (drinking water fountain) is at right center of photograph. Manikin wearing WWII wave uniform is in display case at left center. (045) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
Effect of Adipose-Derived Stem Cells on Head and Neck Squamous Cell Carcinoma.

PubMed

Danan, Deepa; Lehman, Christine E; Mendez, Rolando E; Langford, Brian; Koors, Paul D; Dougherty, Michael I; Peirce, Shayn M; Gioeli, Daniel G; Jameson, Mark J

2018-05-01

Objective Patients with head and neck squamous cell carcinoma (HNSCC) have significant wound-healing difficulties. While adipose-derived stem cells (ASCs) facilitate wound healing, ASCs may accelerate recurrence when applied to a cancer field. This study evaluates the impact of ASCs on HNSCC cell lines in vitro and in vivo. Study Design In vitro experiments using HNSCC cell lines and in vivo mouse experiments. Setting Basic science laboratory. Subjects and Methods Impact of ASCs on in vitro proliferation, survival, and migration was assessed using 8 HNSCC cell lines. One cell line was used in a mouse orthotopic xenograft model to evaluate in vivo tumor growth in the presence and absence of ASCs. Results Addition of ASCs did not increase the number of HNSCC cells. In clonogenic assays to assess cell survival, addition of ASCs increased colony formation only in SCC9 cells (maximal effect 2.3-fold, P < .02) but not in other HNSCC cell lines. In scratch assays to assess migration, fluorescently tagged ASCs did not migrate appreciably and did not increase the rate of wound closure in HNSCC cell lines. Addition of ASCs to HNSCC xenografts did not increase tumor growth. Conclusion Using multiple in vitro and in vivo approaches, ASCs did not significantly stimulate HNSCC cell proliferation or migration and increased survival in only a single cell line. These findings preliminarily suggest that the use of ASCs may be safe in the setting of HNSCC but that further investigation on the therapeutic use of ASCs in the setting of HNSCC is needed.
'Reading the Mind in the Eyes': an fMRI study of adolescents with autism and their siblings.

PubMed

Holt, R J; Chura, L R; Lai, M-C; Suckling, J; von dem Hagen, E; Calder, A J; Bullmore, E T; Baron-Cohen, S; Spencer, M D

2014-11-01

Mentalizing deficits are a hallmark of the autism spectrum condition (ASC) and a potential endophenotype for atypical social cognition in ASC. Differences in performance and neural activation on the 'Reading the Mind in the Eyes' task (the Eyes task) have been identified in individuals with ASC in previous studies. Performance on the Eyes task along with the associated neural activation was examined in adolescents with ASC (n = 50), their unaffected siblings (n = 40) and typically developing controls (n = 40). Based on prior literature that males and females with ASC display different cognitive and associated neural characteristics, analyses were stratified by sex. Three strategies were applied to test for endophenotypes at the level of neural activation: (1) identifying and locating conjunctions of ASC-control and sibling-control differences; (2) examining whether the sibling group is comparable to the ASC or intermediate between the ASC and control groups; and (3) examining spatial overlaps between ASC-control and sibling-control differences across multiple thresholds. Impaired behavioural performance on the Eyes task was observed in males with ASC compared to controls, but only at trend level in females; and no difference in performance was identified between sibling and same-sex control groups in both sexes. Neural activation showed a substantial endophenotype effect in the female groups but this was only modest in the male groups. Behavioural impairment on complex emotion recognition associated with mental state attribution is a phenotypic, rather than an endophenotypic, marker of ASC. However, the neural response during the Eyes task is a potential endophenotypic marker for ASC, particularly in females.
Active symptom control with or without chemotherapy in the treatment of patients with malignant pleural mesothelioma (MS01): a multicentre randomised trial.

PubMed

Muers, Martin F; Stephens, Richard J; Fisher, Patricia; Darlison, Liz; Higgs, Christopher M B; Lowry, Erica; Nicholson, Andrew G; O'Brien, Mary; Peake, Michael; Rudd, Robin; Snee, Michael; Steele, Jeremy; Girling, David J; Nankivell, Matthew; Pugh, Cheryl; Parmar, Mahesh K B

2008-05-17

Malignant pleural mesothelioma is almost always fatal, and few treatment options are available. Although active symptom control (ASC) has been recommended for the management of this disease, no consensus exists for the role of chemotherapy. We investigated whether the addition of chemotherapy to ASC improved survival and quality of life. 409 patients with malignant pleural mesothelioma, from 76 centres in the UK and two in Australia, were randomly assigned to ASC alone (treatment could include steroids, analgesic drugs, bronchodilators, palliative radiotherapy [n=136]); to ASC plus MVP (four cycles of mitomycin 6 mg/m2, vinblastine 6 mg/m2, and cisplatin 50 mg/m2 every 3 weeks [n=137]); or to ASC plus vinorelbine (one injection of vinorelbine 30 mg/m2 every week for 12 weeks [n=136]). Randomisation was done by minimisation, with stratification for WHO performance status, histology, and centre. Follow-up was every 3 weeks to 21 weeks after randomisation, and every 8 weeks thereafter. Because of slow accrual, the two chemotherapy groups were combined and compared with ASC alone for the primary outcome of overall survival. Analysis was by intention to treat. This study is registered, number ISRCTN54469112. At the time of analysis, 393 (96%) patients had died (ASC 132 [97%], ASC plus MVP 132 [96%], ASC plus vinorelbine 129 [95%]). Compared with ASC alone, we noted a small, non-significant survival benefit for ASC plus chemotherapy (hazard ratio [HR] 0.89 [95% CI 0.72-1.10]; p=0.29). Median survival was 7.6 months in the ASC alone group and 8.5 months in the ASC plus chemotherapy group. Exploratory analyses suggested a survival advantage for ASC plus vinorelbine compared with ASC alone (HR 0.80 [0.63-1.02]; p=0.08), with a median survival of 9.5 months. There was no evidence of a survival benefit with ASC plus MVP (HR 0.99 [0.78-1.27]; p=0.95). We observed no between-group differences in four predefined quality-of-life subscales (physical functioning, pain, dyspnoea

PAMAM dendrimer-baculovirus nanocomplex for microencapsulated adipose stem cell-gene therapy: in vitro and in vivo functional assessment.

PubMed

Paul, Arghya; Shao, Wei; Abbasi, Sana; Shum-Tim, Dominique; Prakash, Satya

2012-09-04

The present study aims to develop a new stem cell based gene delivery system consisting of human adipose tissue derived stem cells (hASCs) genetically modified with self-assembled nanocomplex of recombinant baculovirus and PAMAM dendrimer (Bac-PAMAM) to overexpress the vascular endothelial growth factor (VEGF). Cells were enveloped into branched PEG surface functionalized polymeric microcapsules for efficient transplantation. In vitro analysis confirmed efficient transduction of hASCs expressing 7.65 ± 0.86 ng functionally active VEGF per 10(6) microencapsulated hASCs (ASC-VEGF). To determine the potential of the developed system, chronically infarcted rat hearts were treated with either empty microcapsules (MC), microencapsulated hASCs expressing MGFP reporter protein (MC+ASC-MGFP), or MC+ASC-VEGF, and analyzed for 10 weeks. Post-transplantation data confirmed higher myocardial VEGF expressions with significantly enhanced neovasculature in the MC+ASC-VEGF group. In addition, the cardiac performance, as measured by percentage ejection fraction, also improved significantly in the MC+ASC-VEGF group (48.6 ± 6.1%) compared to that in MC+ASC-MGFP (38.8 ± 5.3%) and MC groups (31.5 ± 3.3%). Collectively, these data demonstrate the feasibility of this system for improved stem cell therapy applications.
Angiogenic Synergistic Effect of Adipose-Derived Stromal Cell Spheroids with Low-Level Light Therapy in a Model of Acute Skin Flap Ischemia.

PubMed

Park, In-Su; Chung, Phil-Sang; Ahn, Jin Chul

2016-01-01

Human adipose-derived mesenchymal stem cells (hASCs) are an attractive cell source for tissue engineering. However, one obstacle to this approach is that the transplanted hASC population can decline rapidly in the recipient tissue. The aim of this study was to investigate the effects of low-level light therapy (LLLT) on transplanted spheroid hASCs in skin flaps of mice. hASCs were cultured in monolayers or spheroids. LLLT, hASCs, spheroids and spheroids transplanted with LLLT were applied to the skin flaps. Healing of the skin flaps was assessed by gross evaluation and by hematoxylin and eosin staining and elastin van Gieson staining. Compared with the spheroid group, skin flap healing was enhanced in the spheroid + LLLT group, including the neovascularization and regeneration of skin appendages. The survival of hASCs was enhanced by decreased apoptosis of hASCs in the skin flaps of the spheroid + LLLT group. The secretion of growth factors was stimulated in the spheroid + LLLT group compared with the ASC and spheroid groups. These data suggest that LLLT was an effective biostimulator of spheroid hASCs in the skin flaps, enhancing the survival of hASCs and stimulating the secretion of growth factors. © 2016 S. Karger AG, Basel.
Development of large-scale manufacturing of adipose-derived stromal cells for clinical applications using bioreactors and human platelet lysate.

PubMed

Haack-Sørensen, Mandana; Juhl, Morten; Follin, Bjarke; Harary Søndergaard, Rebekka; Kirchhoff, Maria; Kastrup, Jens; Ekblond, Annette

2018-04-17

In vitro expanded adipose-derived stromal cells (ASCs) are a useful resource for tissue regeneration. Translation of small-scale autologous cell production into a large-scale, allogeneic production process for clinical applications necessitates well-chosen raw materials and cell culture platform. We compare the use of clinical-grade human platelet lysate (hPL) and fetal bovine serum (FBS) as growth supplements for ASC expansion in the automated, closed hollow fibre quantum cell expansion system (bioreactor). Stromal vascular fractions were isolated from human subcutaneous abdominal fat. In average, 95 × 10 6 cells were suspended in 10% FBS or 5% hPL medium, and loaded into a bioreactor coated with cryoprecipitate. ASCs (P0) were harvested, and 30 × 10 6 ASCs were reloaded for continued expansion (P1). Feeding rate and time of harvest was guided by metabolic monitoring. Viability, sterility, purity, differentiation capacity, and genomic stability of ASCs P1 were determined. Cultivation of SVF in hPL medium for in average nine days, yielded 546 × 10 6 ASCs compared to 111 × 10 6 ASCs, after 17 days in FBS medium. ASCs P1 yields were in average 605 × 10 6 ASCs (PD [population doublings]: 4.65) after six days in hPL medium, compared to 119 × 10 6 ASCs (PD: 2.45) in FBS medium, after 21 days. ASCs fulfilled ISCT criteria and demonstrated genomic stability and sterility. The use of hPL as a growth supplement for ASCs expansion in the quantum cell expansion system provides an efficient expansion process compared to the use of FBS, while maintaining cell quality appropriate for clinical use. The described process is an obvious choice for manufacturing of large-scale allogeneic ASC products.
Adipose-derived stem cells retain their regenerative potential after methotrexate treatment

DOE Office of Scientific and Technical Information (OSTI.GOV)

Beane, Olivia S.; Fonseca, Vera C.; Darling, Eric M., E-mail: Eric_Darling@brown.edu

In musculoskeletal tissues like bone, chemotherapy can impair progenitor cell differentiation and proliferation, resulting in decreased bone growth and mineralization throughout a patient's lifetime. In the current study, we investigated the effects of chemotherapeutics on adipose-derived stem cell (ASC) function to determine whether this cell source could be a candidate for repairing, or even preventing, chemotherapy-induced tissue damage. Dose-dependent proliferation rates of ASCs and normal human fibroblasts (NHFs) were quantified after treatment with cytarabine (CY), etoposide (ETO), methotrexate (MTX), and vincristine (VIN) using a fluorescence-based assay. The influence of MTX on the multipotency of ASCs and freshly isolated stromal vascularmore » fraction (SVF) cells was also evaluated using lineage-specific stains and spectrophotometry. ASC and NHF proliferation were equally inhibited by exposure to CY and ETO; however, when treated with MTX and VIN, ASCs exhibited greater resistance. This was especially apparent for MTX-treated samples, with ASC proliferation showing no inhibition for clinically relevant MTX doses ranging from 0.1 to 50 μM. Additional experiments revealed that the differentiation potential of ASCs was not affected by MTX treatment and that upregulation of dihydrofolate reductase possibly contributed to this response. Moreover, SVF cells, which include ASCs, exhibited similar resistance to MTX impairment, with respect to cellular proliferation, clonogenicity, and differentiation capability. Therefore, we have shown that the regenerative properties of ASCs resist the cytotoxicity of MTX, identifying these cells as a potential key for repairing musculoskeletal damage in patients undergoing chemotherapy. - Highlights: • Long-term effects of chemotherapeutics can include musculoskeletal dysfunction. • A screen of common drugs showed disparate effects on ASCs and fibroblasts. • One drug, methotrexate, did not impair ASC growth
Paclitaxel Impairs Adipose Stem Cell Proliferation and Differentiation

PubMed Central

Choron, Rachel L.; Chang, Shaohua; Khan, Sophia; Villalobos, Miguel A.; Zhang, Ping; Carpenter, Jeffrey P.; Tulenko, Thomas N.; Liu, Yuan

2015-01-01

BACKGROUND Cancer patients with chemotherapy-induced immunosuppression have poor surgical site wound healing. Prior literature supports the use of human adipose-derived stem cell (hASC) lipoinjection to improve wound healing. It has been established multipotent hASCs facilitate neovascularization, accelerated epithelialization, and wound closure in animal models. While hASC wound therapy may benefit surgical cancer patients, the chemotherapeutic effects on hASCs are unknown. We hypothesized Paclitaxel, a chemotherapeutic agent, impairs hASC growth, multipotency, and induces apoptosis. METHODS hASCs were isolated and harvested from consented, chemotherapy and radiation naïve patients. Growth curves, MTT, and EdU assays measured cytotoxicity and proliferation. Oil-Red-O stain, Alazarin-Red stain, Matrigel tube-formation assay, and qPCR analyzed hASC differentiation. Annexin V assay measured apoptosis. Immunostaining and Western blot determined TNF-α expression. RESULTS hASCs were selectively more sensitive to Paclitaxel (0.01μM–30μM) than fibroblasts (p<0.05). After 12 days, Paclitaxel caused hASC growth arrest whereas control hASCs proliferated (p=0.006). Paclitaxel caused an 80.6% reduction in new DNA synthesis (p<0.001). Paclitaxel severely inhibited endothelial differentiation and capillary-like tube formation. Differentiation markers LPL (adipogenic), alkaline phosphatase (osteogenic), CD31 and vWF (endothelial) were significantly decreased (all: p<0.05) confirming Paclitaxel impaired differentiation. Paclitaxel was also found to induce apoptosis and TNF-α was up-regulated in Paclitaxel-treated hASCs (p<0.001). CONCLUSION Paclitaxel is more cytotoxic to hASCs than fibroblasts. Paclitaxel inhibits hASC proliferation, differentiation, and induces apoptosis, possibly through the TNF-α pathway. Paclitaxel’s severe inhibition of endothelial differentiation indicates neovascularization disruption, possibly causing poor wound healing in cancer patients
An Eye on Operations.

ERIC Educational Resources Information Center

Barna, Willian C.; Cuzzetto, Charles

1993-01-01

A permanent inhouse auditor at the Tacoma (Washington) school district provides assurance to the administration that complex systems and procedures are effective and that financial reports are accessible. Lists criteria for defining the auditor's role and methods for evaluating the auditor. (MLF)
A Faculty Driven Teaching & Learning Center: The Evolution of a Professional Development Venture.

ERIC Educational Resources Information Center

Sablan, Helen

Tacoma Community College (TCC), in Washington, has implemented a comprehensive professional development program to serve the training and development needs of its employees. Program goals include promoting student success through curriculum review and teaching development, increasing opportunities for professional development, building a positive…
Videotex--The Library of the Future.

ERIC Educational Resources Information Center

Mischo, Lare; Hegarty, Kevin

1982-01-01

Discusses a presentation prepared by Boeing Computer Services in cooperation with the Tacoma Public Library staff, which demonstrates the potential of interactive cable systems based on the Canadian Telidon system. Features of this videotex system, software, and equipment, including microcomputers, are noted. (EJS)
Osteogenic capability of autologous rabbit adipose-derived stromal cells in repairing calvarial defects.

PubMed

Cheng, Shao-Wen; Lin, Zhong-Qin; Wang, Wei; Zhang, Wei; Kou, Dong-Quan; Ying, Xiao-Zhou; Chen, Qing-Yu; Shen, Yue; Cheng, Xiao-Jie; Peng, Lei; Lv, Chuan-Zhu

2011-01-01

To evaluate the in vitro and in vivo osteogenic capability of adipose-derived stromal cells (ASCs). ASCs were isolated from New Zealand white rabbits and determined by alkaline phosphatase (ALP) staining, von Kossa staining and alizarin red staining. Some specific markers of osteogenic differentiation, including ALP, osteocalcin (OCN), osteopontin (OPN) were examined by reverse transcription-polymerase chain reaction (RT-PCR). In vivo, demineralized bone matrix (DBM)-ASCs composites were implanted into the rabbit calvarial defects created at each side of the longitudinal midline. After 6 weeks, histologic properties of the transplants were analyzed. ASCs were successfully induced into osteogenesis. ALP staining, von Kossa staining and alizarin red staining showed positive results. The expressions of ALP, OCN and OPN were detected in ASCs after cultivation in osteogenic medium. Extensive new bone was observed in the defects transplanted with DBM-ASCs composites. ASCs have the potential to differentiate into osteogenic lineage and DBM-ASCs constructs are a promising method for regeneration in bone defects.
The distribution of SIgA and IgG antibody-secreting cells in the palatine tonsils of Bactrian camels (Camelus bactrianus) of different ages.

PubMed

Jia, Shuai; Zhang, Wangdong; Tan, Xuefen; He, Wanhong; Wang, Wenhui

2017-05-01

Secretory immunoglobulin A (SIgA) antibody-secreting cells (ASCs) are the major effector cells of mucosal immunity, and immunoglobulin G (IgG) ASCs are also associated with mucosal immunity. This study aimed to explore the distribution of these 2 ASC populations in the palatine tonsils of Bactrian camels of different ages. Eighteen Bactrian camels were divided into the following three age groups: pubertal (3-5 years), middle-aged (6-16 years) and old (17-20 years). SIgA and IgG ASCs within different sites of the palatine tonsils were observed through histological and immunohistochemical techniques, and their densities were analyzed using statistical methods. The results from all age groups showed that both the SIgA and IgG ASCs were primarily distributed in the subepithelial compartments of the reticulated crypt epithelium and secondarily distributed in the subepithelial compartments of the stratified surface squamous epithelium, with a few ASCs located in the extrafollicular region. Their densities in these three areas were significantly decreased in turn (P<0.05). However, the densities of SIgA ASCs were significantly higher than IgG ASCs in the same regions (P<0.05), and the densities of both ASC populations decreased with age. The results confirmed that Bactrian camel palatine tonsils are the primary mucosal immune organ producing SIgA ASCs, and the subepithelial compartment of the reticulated crypt epithelium is the primary region for the colonization and functional activity of SIgA and IgG ASCs.
26 CFR 1.41-9T - Alternative simplified credit (temporary).

Code of Federal Regulations, 2010 CFR

2010-04-01

... 26 Internal Revenue 1 2010-04-01 2010-04-01 true Alternative simplified credit (temporary). 1.41... INCOME TAXES Credits Against Tax § 1.41-9T Alternative simplified credit (temporary). (a) Determination... provisions of the alternative simplified credit (ASC) in section 41(c)(5) for any taxable year of the...
77 FR 33704 - Inviting Applications for Rural Business Opportunity Grants

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-07

... Development State Office, 1835 Black Lake Boulevard SW., Suite B, Olympia, WA 98512-5715, (360) 704-7740/TDD... at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director...-9410 or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and...
Association of 17-β Estradiol with Adipose-Derived Stem Cells: New Strategy to Produce Functional Myogenic Differentiated Cells with a Nano-Scaffold for Tissue Engineering.

PubMed

Feng, Chunxiang; Hu, Jinqian; Liu, Chang; Liu, Shiliang; Liao, Guiying; Song, Linjie; Zeng, Xiaoyong

2016-01-01

The increased incidence of stress urinary incontinence (SUI) in postmenopausal women has been proposed to be associated with a reduction in the level of 17-β estradiol (E2). E2 has also been shown to enhance the multi-differentiation ability of adipose-derived stem cells (ASCs) in vitro. However, studies on the potential value of E2 for tissue engineering in SUI treatment are rare. In the present study, we successfully fabricated myogenically differentiated ASCs (MD-ASCs), which were seeded onto a Poly(l-lactide)/Poly(e-caprolactone) electrospinning nano-scaffold, and incorporated E2 into the system, with the aim of improving the proliferation and myogenic differentiation of ASCs. ASCs were collected from the inguinal subcutaneous fat of rats. The proliferation and myogenic differentiation of ASCs, as well as the nano-scaffold biocompatibility of MD-ASCs, with or without E2 supplementation, were investigated. We demonstrated that E2 incorporation enhanced the proliferation of ASCs in vitro, and the most optimal concentration was 10-9 M. E2 also led to modulation of the MD-ASCs phenotype toward a concentrated type with smooth muscle-inductive medium. The expression of early (alpha-smooth muscle actin), mid (calponin), and late-stage (myosin heavy chain) contractile markers in MD-ASCs was enhanced by E2 during the different differentiation stages. Furthermore, the nano-scaffold was biocompatible with MD-ASCs, and cell proliferation was significantly enhanced by E2. Taken together, these results demonstrate that E2 can enhance the proliferation and myogenic differentiation of ASCs and can be used to construct a biocompatible cell/nano-scaffold. These scaffolds with desirable differentiation cells show promising applications for tissue engineering.
Mechanisms of vasculogenesis in 3D fibrin matrices mediated by the interaction of adipose-derived stem cells and endothelial cells.

PubMed

Rohringer, Sabrina; Hofbauer, Pablo; Schneider, Karl H; Husa, Anna-Maria; Feichtinger, Georg; Peterbauer-Scherb, Anja; Redl, Heinz; Holnthoner, Wolfgang

2014-10-01

Vascularization of tissue-engineered constructs is essential to provide sufficient nutrient supply and hemostasis after implantation into target sites. Co-cultures of adipose-derived stem cells (ASC) with outgrowth endothelial cells (OEC) in fibrin gels were shown to provide an effective possibility to induce vasculogenesis in vitro. However, the mechanisms of the interaction between these two cell types remain unclear so far. The aim of this study was to evaluate differences of direct and indirect stimulation of ASC-induced vasculogenesis, the influence of ASC on network stabilization and molecular mechanisms involved in vascular structure formation. Endothelial cells (EC) were embedded in fibrin gels either containing non-coated or ASC-coated microcarrier beads as well as ASC alone. Moreover, EC-seeded constructs incubated with ASC-conditioned medium were used in addition to constructs with ASC seeded on top. Vascular network formation was visualized by green fluorescent protein expressing cells or immunostaining for CD31 and quantified. RT-qPCR of cells derived from co-cultures in fibrin was performed to evaluate changes in the expression of EC marker genes during the first week of culture. Moreover, angiogenesis-related protein levels were measured by performing angiogenesis proteome profiler arrays. The results demonstrate that proximity of endothelial cells and ASC is required for network formation and ASC stabilize EC networks by developing pericyte characteristics. We further showed that ASC induce controlled vessel growth by secreting pro-angiogenic and regulatory proteins. This study reveals angiogenic protein profiles involved in EC/ASC interactions in fibrin matrices and confirms the usability of OEC/ASC co-cultures for autologous vascular tissue engineering.
Advanced Stirling Convertor Testing at GRC

NASA Technical Reports Server (NTRS)

Schifer, Nick; Oriti, Salvatore M.

2013-01-01

NASA Glenn Research Center (GRC) has been supporting development of the Advanced Stirling Radioisotope Generator (ASRG) since 2006. A key element of the ASRG project is providing life, reliability, and performance testing of the Advanced Stirling Convertor (ASC). The latest version of the ASC, deemed ASC-E3, is of a design identical to the forthcoming flight convertors. The first pair of ASC-E3 units was delivered in December 2012. GRC has begun the process of adding these units to the catalog of ongoing Stirling convertor operation. This process includes performance verification, which examines the data from various tests to validate the convertors performance to the product specification.
Ultrasound-Assisted Liposuction Does Not Compromise the Regenerative Potential of Adipose-Derived Stem Cells

PubMed Central

Duscher, Dominik; Atashroo, David; Maan, Zeshaan N.; Luan, Anna; Brett, Elizabeth A.; Barrera, Janos; Khong, Sacha M.; Zielins, Elizabeth R.; Whittam, Alexander J.; Hu, Michael S.; Walmsley, Graham G.; Pollhammer, Michael S.; Schmidt, Manfred; Schilling, Arndt F.; Machens, Hans-Günther; Huemer, Georg M.; Wan, Derrick C.; Longaker, Michael T.

2016-01-01

Human mesenchymal stem cells (MSCs) have recently become a focus of regenerative medicine, both for their multilineage differentiation capacity and their excretion of proregenerative cytokines. Adipose-derived mesenchymal stem cells (ASCs) are of particular interest because of their abundance in fat tissue and the ease of harvest via liposuction. However, little is known about the impact of different liposuction methods on the functionality of ASCs. Here we evaluate the regenerative abilities of ASCs harvested via a third-generation ultrasound-assisted liposuction (UAL) device versus ASCs obtained via standard suction-assisted lipoaspiration (SAL). Lipoaspirates were sorted using fluorescent assisted cell sorting based on an established surface-marker profile (CD34+/CD31−/CD45−), to obtain viable ASCs. Yield and viability were compared and the differentiation capacities of the ASCs were assessed. Finally, the regenerative potential of ASCs was examined using an in vivo model of tissue regeneration. UAL- and SAL-derived samples demonstrated equivalent ASC yield and viability, and UAL ASCs were not impaired in their osteogenic, adipogenic, or chondrogenic differentiation capacity. Equally, quantitative real-time polymerase chain reaction showed comparable expression of most osteogenic, adipogenic, and key regenerative genes between both ASC groups. Cutaneous regeneration and neovascularization were significantly enhanced in mice treated with ASCs obtained by either UAL or SAL compared with controls, but there were no significant differences in healing between cell-therapy groups. We conclude that UAL is a successful method of obtaining fully functional ASCs for regenerative medicine purposes. Cells harvested with this alternative approach to liposuction are suitable for cell therapy and tissue engineering applications. Significance Adipose-derived mesenchymal stem cells (ASCs) are an appealing source of therapeutic progenitor cells because of their multipotency
Ultrasound-Assisted Liposuction Does Not Compromise the Regenerative Potential of Adipose-Derived Stem Cells.

PubMed

Duscher, Dominik; Atashroo, David; Maan, Zeshaan N; Luan, Anna; Brett, Elizabeth A; Barrera, Janos; Khong, Sacha M; Zielins, Elizabeth R; Whittam, Alexander J; Hu, Michael S; Walmsley, Graham G; Pollhammer, Michael S; Schmidt, Manfred; Schilling, Arndt F; Machens, Hans-Günther; Huemer, Georg M; Wan, Derrick C; Longaker, Michael T; Gurtner, Geoffrey C

2016-02-01

Human mesenchymal stem cells (MSCs) have recently become a focus of regenerative medicine, both for their multilineage differentiation capacity and their excretion of proregenerative cytokines. Adipose-derived mesenchymal stem cells (ASCs) are of particular interest because of their abundance in fat tissue and the ease of harvest via liposuction. However, little is known about the impact of different liposuction methods on the functionality of ASCs. Here we evaluate the regenerative abilities of ASCs harvested via a third-generation ultrasound-assisted liposuction (UAL) device versus ASCs obtained via standard suction-assisted lipoaspiration (SAL). Lipoaspirates were sorted using fluorescent assisted cell sorting based on an established surface-marker profile (CD34+/CD31-/CD45-), to obtain viable ASCs. Yield and viability were compared and the differentiation capacities of the ASCs were assessed. Finally, the regenerative potential of ASCs was examined using an in vivo model of tissue regeneration. UAL- and SAL-derived samples demonstrated equivalent ASC yield and viability, and UAL ASCs were not impaired in their osteogenic, adipogenic, or chondrogenic differentiation capacity. Equally, quantitative real-time polymerase chain reaction showed comparable expression of most osteogenic, adipogenic, and key regenerative genes between both ASC groups. Cutaneous regeneration and neovascularization were significantly enhanced in mice treated with ASCs obtained by either UAL or SAL compared with controls, but there were no significant differences in healing between cell-therapy groups. We conclude that UAL is a successful method of obtaining fully functional ASCs for regenerative medicine purposes. Cells harvested with this alternative approach to liposuction are suitable for cell therapy and tissue engineering applications. Significance: Adipose-derived mesenchymal stem cells (ASCs) are an appealing source of therapeutic progenitor cells because of their multipotency
Atypical Squamous Cells of Undetermined Significance: Bethesda Classification and Association with Human Papillomavirus

PubMed Central

Barcelos, Ana Cristina Macêdo; Michelin, Márcia Antoniazi; Adad, Sheila Jorge; Murta, Eddie Fernando Candido

2011-01-01

Introduction. To analyze patients with atypical squamous cells of undetermined significance (ASCUS) through a cytology review and the presence of microbiological agents, with consideration of colposcopy and semiannual tracking. Methods. 103 women with ASCUS were reviewed and reclassified: normal/inflammatory, ASCUS, low-grade squamous intraepithelial lesion (LSIL), or high-grade squamous intraepithelial lesion (HSIL). If ASCUS confirmed, it was subclassified in reactive or neoplastic ASCUS, ASC-US, or ASC-H; and Regione Emilia Romagna Screening Protocol. Patients underwent a colposcopic examination, and test for Candida sp., bacterial vaginosis, Trichomonas vaginalis, and human papillomavirus (HPV) were performed. Results. Upon review, ASCUS was diagnosis in 70/103 (67.9%), being 38 (54.2%) reactive ASCUS and 32 (45.71%) neoplastic ASCUS; 62 (88.5%) ASC-US and 8 (11.41%) ASC-H. ASCUS (Regione Protocol), respectively 1-5: 15 (21.4%), 19 (27.1%), 3 (27.1%), 16 (22.8%), and 1 (1.4%). A higher number of cases of cervical intraepithelial neoplasia (CIN) II/III in the biopsies of patients with ASC-H compared to ASC-US (P = .0021). High-risk HPV test and presence of CIN II/III are more frequent in ASC-H than ASC-US (P = .031). Conclusions. ASC-H is associated with clinically significant disease. High-risk HPV-positive status in the triage for colposcopy of patients with ASC-US is associated with increased of CIN. PMID:21760701
Diabetes impairs adipose tissue-derived stem cell function and efficiency in promoting wound healing.

PubMed

Cianfarani, Francesca; Toietta, Gabriele; Di Rocco, Giuliana; Cesareo, Eleonora; Zambruno, Giovanna; Odorisio, Teresa

2013-01-01

Adipose tissue-derived stem cells (ASCs) are gaining increasing consideration in tissue repair therapeutic application. Recent evidence indicates that ASCs enhance skin repair in animal models of impaired wound healing. To assess the therapeutic activity of autologous vs. allogeneic ASCs in the treatment of diabetic ulcers, we functionally characterized diabetic ASCs and investigated their potential to promote wound healing with respect to nondiabetic ones. Adipose tissue-derived cells from streptozotocin-induced type 1 diabetic mice were analyzed either freshly isolated as stromal vascular fraction (SVF), or following a single passage of culture (ASCs). Diabetic ASCs showed decreased proliferative potential and migration. Expression of surface markers was altered in diabetic SVF and cultured ASCs, with a reduction in stem cell marker-positive cells. ASCs from diabetic mice released lower amounts of hepatocyte growth factor, vascular endothelial growth factor (VEGF)-A, and insulin-like growth factor-1, growth factors playing important roles in skin repair. Accordingly, the supernatant of diabetic ASCs manifested reduced capability to promote keratinocyte and fibroblast proliferation and migration. Therapeutic potential of diabetic SVF administered to wounds of diabetic mice was blunted as compared with cells isolated from nondiabetic mice. Our data indicate that diabetes alters ASC intrinsic properties and impairs their function, thus affecting therapeutic potential in the autologous treatment for diabetic ulcers. © 2013 by the Wound Healing Society.
77 FR 63874 - Notice of Determinations Regarding Eligibility To Apply for Worker Adjustment Assistance

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-17

... industry in an investigation resulting in-- (A) an affirmative determination of serious injury or threat..., Tomkins Charleston, MO......... March 29, 2011. Ltd., Industrial and Automotive Group, Manpower. 81,468...., Tacoma, WA On-Site Leased Workers of Manpower. 81,317 Dana Holding Corporation, Milwaukee, WI Power...

"Tengo Que Habla Español. Yo No Entiendo Ingles!": A Qualitative Case Study on a Bilingual Child with Autism Spectrum Conditions

ERIC Educational Resources Information Center

Kim, Hyun Uk; Roberti, Marilyn

2014-01-01

Language differences in children with Autism Spectrum Conditions (ASC) often lead professionals to believe that children with ASC cannot or should not become bilingual, thus advising parents with a child with ASC raised in a bilingual household to adhere to English only. Emerging studies, however, attest that children with ASC can become…
Olfactory Detection Thresholds and Adaptation in Adults with Autism Spectrum Condition

ERIC Educational Resources Information Center

Tavassoli, T.; Baron-Cohen, S.

2012-01-01

Sensory issues have been widely reported in Autism Spectrum Conditions (ASC). Since olfaction is one of the least investigated senses in ASC, the current studies explore olfactory detection thresholds and adaptation to olfactory stimuli in adults with ASC. 80 participants took part, 38 (18 females, 20 males) with ASC and 42 control participants…
Human adipose derived mesenchymal stromal cells transduced with GFP lentiviral vectors: assessment of immunophenotype and differentiation capacity in vitro.

PubMed

van Vollenstee, Fiona A; Jackson, Carlo; Hoffmann, Danie; Potgieter, Marnie; Durandt, Chrisna; Pepper, Michael S

2016-10-01

Adipose derived mesenchymal stromal/stem cells (ASCs) are a heterogeneous population characterized by (a) their ability to adhere to plastic; (b) immunophenotypic expression of certain cell surface markers, while lacking others; and (c) the capacity to differentiate into lineages of mesodermal origin including osteocytes, chondrocytes and adipocytes. The long-term goal is to utilize these cells for clinical translation into cell-based therapies. However, preclinical safety and efficacy need to be demonstrated in animal models. ASCs can also be utilized as biological vehicles for vector-based gene delivery systems, since they are believed to home to sites of inflammation and infection in vivo. These factors motivated the development of a labelling system for ASCs using lentiviral vector-based green fluorescent protein (GFP) transduction. Human ASCs were transduced with GFP-expressing lentiviral vectors. A titration study determined the viral titer required to transduce the maximum number of ASCs. The effect of the transduced GFP lentiviral vector on ASC immunophenotypic expression of surface markers as well as their ability to differentiate into osteocytes and adipocytes were assessed in vitro. A transduction efficiency in ASC cultures of approximately 80 % was observed with an MOI of ~118. No significant immunophenotypic differences were observed between transduced and non-transduced cells and both cell types successfully differentiated into adipocytes and osteocytes in vitro. We obtained >80 % transduction of ASCs using GFP lentiviral vectors. Transduced ASCs maintained plastic adherence, demonstrated ASC immunophenotype and the ability to differentiate into cells of the mesodermal lineage. This GFP-ASC transduction technique offers a potential tracking system for future pre-clinical studies.
77 FR 65103 - Rules of Practice in Proceedings Relative to Administrative Offsets Initiated Against Former...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-25

.... 0 3. Section 966.3 is revised to read as follows: Sec. 966.3 Definitions. (a) Accounting Service... the Treasury, found at 31 CFR parts 900-904. Changes to Sec. 966.3 update the definitions of part 966 to refer to the Postal Service Accounting Service Center (ASC) or successor installation instead of...
Baghdadite ceramics modulate the cross talk between human adipose stem cells and osteoblasts for bone regeneration.

PubMed

Lu, Zufu; Wang, Guocheng; Roohani-Esfahani, Iman; Dunstan, Colin R; Zreiqat, Hala

2014-03-01

Understanding interactions among the three elements (cells, scaffolds, and bioactive factors) is critical for successful tissue engineering. This study was aimed to investigate how scaffolds would affect osteogenic gene expression in human adipose tissue-derived stem cells (ASCs) or human primary osteoblasts (HOBs), and their cross talk. Either ASCs or HOBs were seeded on Baghdadite (Ca3ZrSi2O9) and hydroxyapatite/tricalcium phosphate (HA/TCP) scaffolds, and osteogenic gene expression was assessed. To further evaluate how substrate affected HOB and ASC cross talk, an indirect co-culture system with semipermeable inserts placed on the culture plate was set up to co-culture ASCs or HOBs, which were grown in monolayer or seeded on Baghdadite or HA/TCP scaffolds, and osteogenic differentiation of the cells was assessed. We found that Baghdadite scaffolds induced a significantly greater increase in RUNX2, osteopontin, bone sialoprotein, and osteocalcin gene expression in HOBs in comparison to HA/TCP scaffolds; Baghdadite scaffolds also significantly induced RUNX2 and osteopontin, but not bone sialoprotein and osteocalcin gene expression in ASCs. In the co-culture system, the HOBs on Baghdadite scaffolds more markedly promoted osteogenic gene expression in ASCs compared to HOBs in monolayer or the HOBs on HA/TCP scaffolds. In addition, the ASCs seeded on Baghdadite scaffolds more markedly promoted osteogenic gene expression in HOBs than did the ASCs on HA/TCP scaffolds. BMP-2 expression in ASCs or HOBs was increased when they were seeded on Baghdadite scaffolds, and adding Noggin into the co-culture medium largely abrogated Baghdadite scaffold-modulated ASC-HOB cross talk. In summary, Baghdadite scaffolds not only promote the osteogenic differentiation of HOBs or ASCs but also modulate the cross talk between ASCs and HOBs, in part via increasing BMP2 expression, thereby promoting their osteogenic differentiation.
Dura Mater Stimulates Human Adipose-Derived Stromal Cells to Undergo Bone Formation in Mouse Calvarial Defects

PubMed Central

Levi, Benjamin; Nelson, Emily R.; Li, Shuli; James, Aaron W.; Hyun, Jeong S.; Montoro, Daniel T.; Lee, Min; Glotzbach, Jason P.; Commons, George W.; Longaker, Michael T.

2015-01-01

Human adipose-derived stromal cells (hASCs) have a proven capacity to aid in osseous repair of calvarial defects. However, the bone defect microenvironment necessary for osseous healing is not fully understood. In this study, we postulated that the cell-cell interaction between engrafted ASCs and host dura mater (DM) cells is critical for the healing of calvarial defects. hASCs were engrafted into critical sized calvarial mouse defects. The DM-hASC interaction was manipulated surgically by DM removal or by insertion of a semipermeable or nonpermeable membrane between DM and hASCs. Radiographic, histologic, and gene expression analyses were performed. Next, the hASC-DM interaction is assessed by conditioned media (CM) and coculture assays. Finally, bone morphogenetic protein (BMP) signaling from DM was investigated in vivo using novel BMP-2 and anti-BMP-2/4 slow releasing scaffolds. With intact DM, osseous healing occurs both from host DM and engrafted hASCs. Interference with the DM-hASC interaction dramatically reduced calvarial healing with abrogated BMP-2–Smad-1/5 signaling. Using CM and coculture assays, mouse DM cells stimulated hASC osteogenesis via BMP signaling. Through in vivo manipulation of the BMP-2 pathway, we found that BMP-2 plays an important role in DM stimulation of hASC osteogenesis in the context of calvarial bone healing. BMP-2 supplementation to a defect with disrupted DM allowed for bone formation in a nonhealing defect. DM is an osteogenic cell type that both participates in and stimulates osseous healing in a hASC-engrafted calvarial defect. Furthermore, DM-derived BMP-2 paracrine stimulation appears to play a key role for hASC mediated repair. PMID:21656608
l-Ascorbic acid 2-phosphate promotes elongation of hair shafts via the secretion of insulin-like growth factor-1 from dermal papilla cells through phosphatidylinositol 3-kinase.

PubMed

Kwack, M H; Shin, S H; Kim, S R; Im, S U; Han, I S; Kim, M K; Kim, J C; Sung, Y K

2009-06-01

l-Ascorbic acid 2-phosphate (Asc 2-P), a derivative of l-ascorbic acid, promotes elongation of hair shafts in cultured human hair follicles and induces hair growth in mice. To investigate whether the promotion of hair growth by Asc 2-P is mediated by insulin-like growth factor-1 (IGF-1) and, if so, to investigate the mechanism of the Asc 2-P-induced IGF-1 expression. Dermal papilla (DP) cells were cultured and IGF-1 level was measured by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay after Asc 2-P treatment in the absence or presence of LY294002, a phosphatidylinositol 3-kinase (PI3K) inhibitor. Also, hair shaft elongation in cultured human scalp hair follicles and proliferation of cocultured keratinocytes were examined after Asc 2-P treatment in the absence or presence of neutralizing antibody against IGF-1. In addition, keratinocyte proliferation in cultured hair follicles after Asc 2-P treatment in the absence or presence of LY294002 was examined by Ki-67 immunostaining. IGF-1 mRNA in DP cells was upregulated and IGF-1 protein in the conditioned medium of DP cells was significantly increased after treatment with Asc 2-P. Immunohistochemical staining showed that IGF-1 staining is increased in the DP of cultured human hair follicles by Asc 2-P. The neutralizing antibody against IGF-1 significantly suppressed the Asc 2-P-mediated elongation of hair shafts in hair follicle organ culture and significantly attenuated Asc 2-P-induced growth of cocultured keratinocytes. LY294002 significantly attenuated Asc 2-P-inducible IGF-1 expression and proliferation of follicular keratinocytes in cultured hair follicles. These data show that Asc 2-P-inducible IGF-1 from DP cells promotes proliferation of follicular keratinocytes and stimulates hair follicle growth in vitro via PI3K.
Sex differences in frontal lobe connectivity in adults with autism spectrum conditions.

PubMed

Zeestraten, E A; Gudbrandsen, M C; Daly, E; de Schotten, M T; Catani, M; Dell'Acqua, F; Lai, M-C; Ruigrok, A N V; Lombardo, M V; Chakrabarti, B; Baron-Cohen, S; Ecker, C; Murphy, D G M; Craig, M C

2017-04-11

Autism spectrum conditions (ASC) are more prevalent in males than females. The biological basis of this difference remains unclear. It has been postulated that one of the primary causes of ASC is a partial disconnection of the frontal lobe from higher-order association areas during development (that is, a frontal 'disconnection syndrome'). Therefore, in the current study we investigated whether frontal connectivity differs between males and females with ASC. We recruited 98 adults with a confirmed high-functioning ASC diagnosis (61 males: aged 18-41 years; 37 females: aged 18-37 years) and 115 neurotypical controls (61 males: aged 18-45 years; 54 females: aged 18-52 years). Current ASC symptoms were evaluated using the Autism Diagnostic Observation Schedule (ADOS). Diffusion tensor imaging was performed and fractional anisotropy (FA) maps were created. Mean FA values were determined for five frontal fiber bundles and two non-frontal fiber tracts. Between-group differences in mean tract FA, as well as sex-by-diagnosis interactions were assessed. Additional analyses including ADOS scores informed us on the influence of current ASC symptom severity on frontal connectivity. We found that males with ASC had higher scores of current symptom severity than females, and had significantly lower mean FA values for all but one tract compared to controls. No differences were found between females with or without ASC. Significant sex-by-diagnosis effects were limited to the frontal tracts. Taking current ASC symptom severity scores into account did not alter the findings, although the observed power for these analyses varied. We suggest these findings of frontal connectivity abnormalities in males with ASC, but not in females with ASC, have the potential to inform us on some of the sex differences reported in the behavioral phenotype of ASC.
Human adipose-derived stem cell spheroid treated with photobiomodulation irradiation accelerates tissue regeneration in mouse model of skin flap ischemia.

PubMed

Park, In-Su; Chung, Phil-Sang; Ahn, Jin Chul; Leproux, Anais

2017-11-01

Skin flap grafting is a form of transplantation widely used in plastic surgery. However, ischemia/reperfusion injury is the main factor which reduces the survival rate of flaps following grafting. We investigated whether photobiomodulation (PBM) precondition prior to human adipose-derived stromal cell (hASC) spheroid (PBM-spheroid) transplantation improved skin tissue functional recovery by the stimulation of angiogenesis and tissue regeneration in skin flap of mice. The LED had an emission wavelength peaked at 660 ± 20 nm (6 J/cm 2 , 10 mW/cm 2 ). The expression of angiogenic growth factors in PBM-spheroid hASCs was much greater than that of not-PBM-treated spheroid or monolayer-cultured hASCs. From immunochemical staining analysis, the hASCs of PBM-spheroid were CD31 + , KDR + , and CD34 + , whereas monolayer-cultured hASCs were negative for these markers. To evaluate the therapeutic effect of hASC PBM-spheroid in vivo, PBS, monolayer-cultured hASCs, and not-PBM-spheroid were transplanted into a skin flap model. The animals were observed for 14 days. The PBM-spheroid hASCs transplanted into the skin flap ischemia differentiated into endothelial cells and remained differentiated. Transplantation of PBM-spheroid hASCs into the skin flap ischemia significantly elevated the density of vascular formations through angiogenic factors released by the skin flap ischemia and enhanced tissue regeneration at the lesion site. Consistent with these results, the transplantation of PBM-spheroid hASCs significantly improved functional recovery compared with PBS, monolayer-cultured hASCs, and not-PBM-spheroid treatment. These findings suggest that transplantation of PBM-spheroid hASCs may be an effective stem cell therapy for the treatment of skin flap ischemia.
77 FR 27357 - IFR Altitudes; Miscellaneous Amendments

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-10

... Hygen, CO FIX *Allan, CO FIX **13500 *16000--MRA *15400--MCA ALLAN, CO FIX, NW BND **12900--MOCA *CHART... *Allan, CO FIX Laramie, WY VOR/DME..... 16000 *16000--MRA Sec. 95.6106 VOR Federal Airway V106 Is Amended... in Part Olympia, WA VORTAC *Carro, WA FIX **4000 *4000--MRA **2000--MOCA *CARRO, WA FIX Diggn, WA FIX...
Effects of forest harvesting, logging debris, and herbicides on the composition, diversity and assembly of a western Washington, USA plant community

Treesearch

David H. Peter; Timothy B. Harrington

2018-01-01

We examined plant community organization over the first five growing seasons after clearcut harvesting with retention of two levels of logging debris (light and heavy) and application of four vegetation control treatments (non-sprayed control, aminopyralid (A), triclopyr (T), and A+T). Our study site was 44 km northwest of Olympia, WA., USA, and before forest...
Supplement Analysis for the Transmission System Vegetation Management Program FEIS (DOE/EIS-0285/SA-56)

DOE Office of Scientific and Technical Information (OSTI.GOV)

Stratton, Elaine

2002-04-09

Vegetation Management for the following electric yards located in the Ross District: Cape Horn, North Bonneville, Stevenson, Carson, Underwood, Troutdale, Cascade Locks, Acton Ross 345 kilovolts and J.D., Alcoa, Sifton, St. Johns, Ostrander, McLoughlin. BPA proposes to maintain a weed-free environment in the electrical substations located within the Olympia Region’s Ross District.
Durability of exterior natural wood finishes in the Pacific Northwest

Treesearch

William C. Feist; Edward A. Mraz

1980-01-01

There is a growing demand for natural exterior wood finishes that retain the original attractive appearance of wood with the least change in color and masking of grain. A number of experimental finishes are being evaluated for their performance in the cool, moist climate of Olympia, Wash. This study, started in 1966, has included observations on the weathering...
View port to starboard of a portion of compartment A126 ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View port to starboard of a portion of compartment A-126 fitted out to represent a WW I canteen. This may have been location for ship's store where crew could buy tobacco and sundries. Note wood conduit on overhead which served as a chase for wiring. (036) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
View forward from stern showing skylight with rippled glass over ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View forward from stern showing skylight with rippled glass over compartment c-110, officer's quarters; note manually operated capstan at center, and simulated eight inch guns in sheet metal mock-up turret; also note five inch guns in sponsons port and starboard. (p37) - USS Olympia, Penn's Landing, 211 South Columbus Boulevard, Philadelphia, Philadelphia County, PA
[Use of adipose-derived stem cells in an experimental rotator cuff fracture animal model].

PubMed

Barco, R; Encinas, C; Valencia, M; Carrascal, M T; García-Arranz, M; Antuña, S

2015-01-01

Rotator cuff repairs have shown a high level of re-ruptures. We hypothesized that the use of adipose-derived stem cells (ASC) could improve the biomechanical and histological properties of the repair. Controlled experimental study conducted on 44 BDIX rats with section and repair of the supraspinatus tendon and randomization to one of three groups: group A, no intervention (control); group B, local applications of a fibrin sealant; and group C, application of the fibrin sealant with 2 x 10(6) ASC. At 4 and 8 weeks a biomechanical and histological analysis was performed. There were no differences in load-to-failure at 4 and 8 weeks between groups. The load-to-failure did increase between week 4 and week 8. Histologically the tendon-to bone union showed a disorganized fibrovascular tissue. Group C showed a different inflammatory pattern, with less presence of neutrophils and more presence of plasma cells. The use of ASC does not improve the biomechanical or histological properties of the repair site. More studies are needed to improve techniques that enhance the healing site of the repair. Copyright © 2014 SECOT. Published by Elsevier Espana. All rights reserved.
Taste identification in adults with autism spectrum conditions.

PubMed

Tavassoli, T; Baron-Cohen, S

2012-07-01

Sensory issues are widely reported in Autism Spectrum Conditions (ASC). Since taste perception is one of the least studied senses in ASC we explored taste identification in adults with ASC (12 males, 11 females) compared to control participants (14 males, 12 females). 'Taste strips' were used to measure taste identification overall, as well as bitter, sour, sweet and salty tastes. Results revealed lower taste scores overall in the ASC group, as well as for bitter, sour and sweet tastes. Salty taste scores did not differ between the groups. Examining error types showed that adults with ASC more often misidentified a taste as salty or as no taste. Future studies should investigate underlying mechanisms of taste identification difficulties in ASC.
The Internal/External Frame of Reference of Academic Self-Concept: Extension to a Foreign Language and the Role of Language of Instruction

ERIC Educational Resources Information Center

Xu, Man K.; Marsh, Herbert W.; Hau, Kit-Tai; Ho, Irene T.; Morin, Alexandre J. S.; Abduljabbar, Adel S.

2013-01-01

The internal/external frame of reference (I/E) model (Marsh, 1986) posits that the effects of contrasting math and verbal domains of achievement are positive for matching academic self-concepts (ASCs) but negative for nonmatching ASCs (i.e., math achievement on verbal ASC; verbal achievement on math ASC). We extend the classic I/E model by…
Ambulatory Surgery Centers and Their Intended Effects on Outpatient Surgery.

PubMed

Hollenbeck, Brent K; Dunn, Rodney L; Suskind, Anne M; Strope, Seth A; Zhang, Yun; Hollingsworth, John M

2015-10-01

To assess the impact of ambulatory surgery centers (ASCs) on rates of hospital-based outpatient procedures and adverse events. Twenty percent national sample of Medicare beneficiaries. A retrospective study of beneficiaries undergoing outpatient surgery between 2001 and 2010. Health care markets were sorted into three groups-those with ASCs, those without ASCs, and those where one opened for the first time. Generalized linear mixed models were used to assess the impact of ASC opening on rates of hospital-based outpatient surgery, perioperative mortality, and hospital admission. Adjusted hospital-based outpatient surgery rates declined by 7 percent, or from 2,333 to 2,163 procedures per 10,000 beneficiaries, in markets where an ASC opened for the first time (p < .001 for test between slopes). Within these markets, procedure use at ASCs outpaced the decline observed in the hospital setting. Perioperative mortality and admission rates remained flat after ASC opening (both p > .4 for test between slopes). The opening of an ASC in a Hospital Service Area resulted in a decline in hospital-based outpatient surgery without increasing mortality or admission. In markets where facilities opened, procedure growth at ASCs was greater than the decline in outpatient surgery use at their respective hospitals. © Health Research and Educational Trust.
Victimization and Perpetration Experiences of Adults With Autism.

PubMed

Weiss, Jonathan A; Fardella, Michelle A

2018-01-01

This study aimed to describe the self-reported experiences of childhood and adult victimization and perpetration in adults with autism spectrum conditions (ASC) compared to a matched sample, and how victimization and perpetration are associated with autism-related difficulties. Forty-five adults with ASC and 42 adults without ASC completed questionnaires regarding violence victimization and perpetration, emotion regulation, and sociocommunicative competence. Participants with ASC reported experiencing, as children, more overall victimization; specifically, more property crime, maltreatment, teasing/emotional bullying, and sexual assault by peers, compared to participants without ASC. Participants with ASC also reported experiencing more teasing/emotional bullying in adulthood and greater sexual contact victimization. No significant differences were found between groups on perpetration. Sociocommunicative ability and emotion regulation deficits did not explain the heightened risk for victimization. Individuals with ASC have an increased vulnerability to violence victimization, which speaks to the need for interventions, and proactive prevention strategies.

Self-assembled adult adipose-derived stem cell spheroids combined with biomaterials promote wound healing in a rat skin repair model.

PubMed

Hsu, Shan-Hui; Hsieh, Pai-Shan

2015-01-01

Adult adipose-derived stem cells (ASCs) are a type of multipotent mesenchymal stem cells (MSCs) with easy availability and serve as a potential cell source for cell-based therapy. Three-dimensional MSC spheroids may be derived from the self-assembly of individual MSCs grown on certain polymer membranes. In this study, we demonstrated that the self-assembled ASC spheroids on chitosan-hyaluronan membranes expressed more cytokine genes (fibroblast growth factor 1, vascular endothelial growth factor, and chemokine [C-C motif] ligand 2) as well as migration-associated genes (chemokine [C-X-C motif] receptor type 4 and matrix metalloprotease 1) compared with ASC dispersed single cells grown on culture dish. To evaluate the in vivo effects of these spheroids, we applied ASC single cells and ASC spheroids in a designed rat skin repair model. Wounds of 15 × 15 mm were created on rat dorsal skin, where ASCs were administered and covered with hyaluronan gel/chitosan sponge to maintain a moist environment. Results showed that skin wounds treated with ASC spheroids had faster wound closure and a significantly higher ratio of angiogenesis. Tracking of fluorescently labeled ASCs showed close localization of ASC spheroids to microvessels, suggesting enhanced angiogenesis through paracrine effects. Based on the in vitro and in vivo results, the self-assembled ASC spheroids may be a promising cellular source for skin tissue engineering and wound regeneration. © 2014 by the Wound Healing Society.
Impact of low oxygen tension on stemness, proliferation and differentiation potential of human adipose-derived stem cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Choi, Jane Ru; Pingguan-Murphy, Belinda; Wan Abas, Wan Abu Bakar

2014-05-30

Highlights: • Hypoxia maintains the stemness of adipose-derived stem cells (ASCs). • ASCs show an increased proliferation rate under low oxygen tension. • Oxygen level as low as 2% enhances the chondrogenic differentiation potential of ASCs. • HIF-1α may regulate the proliferation and differentiation activities of ASCs under hypoxia. - Abstract: Adipose-derived stem cells (ASCs) have been found adapted to a specific niche with low oxygen tension (hypoxia) in the body. As an important component of this niche, oxygen tension has been known to play a critical role in the maintenance of stem cell characteristics. However, the effect of O{submore » 2} tension on their functional properties has not been well determined. In this study, we investigated the effects of O{sub 2} tension on ASCs stemness, differentiation and proliferation ability. Human ASCs were cultured under normoxia (21% O{sub 2}) and hypoxia (2% O{sub 2}). We found that hypoxia increased ASC stemness marker expression and proliferation rate without altering their morphology and surface markers. Low oxygen tension further enhances the chondrogenic differentiation ability, but reduces both adipogenic and osteogenic differentiation potential. These results might be correlated with the increased expression of HIF-1α under hypoxia. Taken together, we suggest that growing ASCs under 2% O{sub 2} tension may be important in expanding ASCs effectively while maintaining their functional properties for clinical therapy, particularly for the treatment of cartilage defects.« less
Test Rack Development for Extended Operation of Advanced Stirling Convertors at NASA Glenn Research Center

NASA Technical Reports Server (NTRS)

Dugala, Gina M.

2010-01-01

The U.S. Department of Energy, Lockheed Martin Space Systems Company, Sunpower Inc., and NASA Glenn Research Center (GRC) have been developing an Advanced Stirling Radioisotope Generator (ASRG) for use as a power system on space science missions. This generator will make use of free-piston Stirling convertors to achieve higher conversion efficiency than with currently available alternatives. One part of NASA GRC's support of ASRG development includes extended operation testing of Advanced Stirling Convertors (ASCs) developed by Sunpower Inc. and GRC. The ASC consists of a free-piston Stirling engine integrated with a linear alternator. NASA GRC has been building test facilities to support extended operation of the ASCs for several years. Operation of the convertors in the test facility provides convertor performance data over an extended period of time. One part of the test facility is the test rack, which provides a means for data collection, convertor control, and safe operation. Over the years, the test rack requirements have changed. The initial ASC test rack utilized an alternating-current (AC) bus for convertor control; the ASRG Engineering Unit (EU) test rack can operate with AC bus control or with an ASC Control Unit (ACU). A new test rack is being developed to support extended operation of the ASC-E2s with higher standards of documentation, component selection, and assembly practices. This paper discusses the differences among the ASC, ASRG EU, and ASC-E2 test racks.
Adipose Stem Cell Therapy Mitigates Chronic Pancreatitis via Differentiation into Acinar-like Cells in Mice.

PubMed

Sun, Zhen; Gou, Wenyu; Kim, Do-Sung; Dong, Xiao; Strange, Charlie; Tan, Yu; Adams, David B; Wang, Hongjun

2017-11-01

The objective of this study was to assess the capacity of adipose-derived mesenchymal stem cells (ASCs) to mitigate disease progression in an experimental chronic pancreatitis mouse model. Chronic pancreatitis (CP) was induced in C57BL/6 mice by repeated ethanol and cerulein injection, and mice were then infused with 4 × 10 5 or 1 × 10 6 GFP + ASCs. Pancreas morphology, fibrosis, inflammation, and presence of GFP + ASCs in pancreases were assessed 2 weeks after treatment. We found that ASC infusion attenuated pancreatic damage, preserved pancreas morphology, and reduced pancreatic fibrosis and cell death. GFP + ASCs migrated to pancreas and differentiated into amylase + cells. In further confirmation of the plasticity of ASCs, ASCs co-cultured with acinar cells in a Transwell system differentiated into amylase + cells with increased expression of acinar cell-specific genes including amylase and chymoB1. Furthermore, culture of acinar or pancreatic stellate cell lines in ASC-conditioned medium attenuated ethanol and cerulein-induced pro-inflammatory cytokine production in vitro. Our data show that a single intravenous injection of ASCs ameliorated CP progression, likely by directly differentiating into acinar-like cells and by suppressing inflammation, fibrosis, and pancreatic tissue damage. These results suggest that ASC cell therapy has the potential to be a valuable treatment for patients with pancreatitis. Copyright © 2017 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.
Assessment of Student Learning: How Two Paths Forge into One.

ERIC Educational Resources Information Center

Sonandre, Debbie M. A.

2002-01-01

Discusses the history, struggles, and successes of assessment work done at Tacoma Community College (TCC) (Washington). Demonstrates the state and national work TCC has accomplished and the partnerships that have been formed with other institutions as a result of the work. Offers assessment grid example. (NB)
A DESCRIPTIVE AND COMPARATIVE STUDY OF THE ADMINISTRATIVE PATTERNS OPERATIVE IN SIX SCHOOL HEALTH PROGRAMS. FINAL REPORT.

ERIC Educational Resources Information Center

MAYSHARK, CYRUS

FOUR HYPOTHESES RELATED TO SCHOOL HEALTH PROGRAMS IN SIX SELECTED COMMUNITIES--DENVER (COLORADO), DUVAL COUNTY (FLORIDA), EVANSTON (ILLINOIS), PORTLAND (OREGON), PRINCE GEORGE'S COUNTY (MARYLAND), AND TACOMA (WASHINGTON)--WERE TESTED THROUGH ANALYSIS OF INTERVIEW AND QUESTIONNAIRE RESPONSE DATA. OF 321 QUESTIONNAIRES MAILED TO A SELECTED SAMPLE OF…
76 FR 43710 - Notice of Inventory Completion: Slater Museum of Natural History, University of Puget Sound...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-21

... Museum of Natural History, University of Puget Sound, Tacoma, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Slater Museum of Natural History, University of Puget Sound has completed an... contact the Slater Museum of Natural History, University of Puget Sound. Disposition of the human remains...
78 FR 19299 - Notice of Inventory Completion: Slater Museum of Natural History, University of Puget Sound...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-29

... made by the Slater Museum of Natural History and Burke Museum professional staff in consultation with....R50000] Notice of Inventory Completion: Slater Museum of Natural History, University of Puget Sound, Tacoma, WA AGENCY: National Park Service, Interior. ACTION: Notice. SUMMARY: The Slater Museum of Natural...
Environmental enrichment synergistically improves functional recovery by transplanted adipose stem cells in chronic hypoxic-ischemic brain injury.

PubMed

Seo, Jung Hwa; Kim, Hyongbum; Park, Eun Sook; Lee, Jong Eun; Kim, Dong Wook; Kim, Hyun Ok; Im, Sang Hee; Yu, Ji Hea; Kim, Ji Yeon; Lee, Min-Young; Kim, Chul Hoon; Cho, Sung-Rae

2013-01-01

We investigated the effects of environmental enrichment (EE) on the function of transplanted adipose stem cells (ASCs) and the combined effect of EE and ASC transplantation on neurobehavioral function in an animal model of chronic hypoxic-ischemic (HI) brain injury. HI brain damage was induced in 7-day-old mice by unilateral carotid artery ligation and exposure to hypoxia (8% O2 for 90 min). At 6 weeks of age, the mice were randomly injected with either ASCs or PBS into the striatum and were randomly assigned to either EE or standard cages (SC), comprising ASC-EE (n=18), ASC-SC (n=19), PBS-EE (n=12), PBS-SC (n=17), and untreated controls (n=23). Rotarod, forelimb-use asymmetry, and grip strength tests were performed to evaluate neurobehavioral function. The fate of transplanted cells and the levels of endogenous neurogenesis, astrocyte activation, and paracrine factors were also measured. As a result, EE and ASC transplantation synergistically improved rotarod latency, forelimb-use asymmetry, and grip strength compared to those of the other groups. The number of engrafted ASCs and βIII-tubulin(+) neurons derived from the transplanted ASCs was significantly higher in mice in EE than those in SC. EE and ASC transplantation also synergistically increased BrdU(+)βIII-tubulin(+) neurons, GFAP(+) astrocytic density, and fibroblast growth factor 2 (FGF2) level but not the level of CS-56(+) glial scarring in the striatum. In conclusion, EE and ASC transplantation synergistically improved neurobehavioral functions. The underlying mechanisms of this synergism included enhanced repair processes such as higher engraftment of the transplanted ASCs, increased endogenous neurogenesis and astrocytic activation coupled with upregulation of FGF2.
42 CFR 416.140 - Surveys.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 3 2014-10-01 2014-10-01 false Surveys. 416.140 Section 416.140 Public Health... Furnished Before January 1, 2008 § 416.140 Surveys. (a) Timing, purpose, and procedures. (1) No more often than once a year, CMS conducts a survey of a randomly selected sample of participating ASCs to collect...
42 CFR 416.140 - Surveys.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 3 2013-10-01 2013-10-01 false Surveys. 416.140 Section 416.140 Public Health... Furnished Before January 1, 2008 § 416.140 Surveys. (a) Timing, purpose, and procedures. (1) No more often than once a year, CMS conducts a survey of a randomly selected sample of participating ASCs to collect...
42 CFR 416.140 - Surveys.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 3 2012-10-01 2012-10-01 false Surveys. 416.140 Section 416.140 Public Health... Furnished Before January 1, 2008 § 416.140 Surveys. (a) Timing, purpose, and procedures. (1) No more often than once a year, CMS conducts a survey of a randomly selected sample of participating ASCs to collect...
Victimization and Perpetration Experiences of Adults With Autism

PubMed Central

Weiss, Jonathan A.; Fardella, Michelle A.

2018-01-01

This study aimed to describe the self-reported experiences of childhood and adult victimization and perpetration in adults with autism spectrum conditions (ASC) compared to a matched sample, and how victimization and perpetration are associated with autism-related difficulties. Forty-five adults with ASC and 42 adults without ASC completed questionnaires regarding violence victimization and perpetration, emotion regulation, and sociocommunicative competence. Participants with ASC reported experiencing, as children, more overall victimization; specifically, more property crime, maltreatment, teasing/emotional bullying, and sexual assault by peers, compared to participants without ASC. Participants with ASC also reported experiencing more teasing/emotional bullying in adulthood and greater sexual contact victimization. No significant differences were found between groups on perpetration. Sociocommunicative ability and emotion regulation deficits did not explain the heightened risk for victimization. Individuals with ASC have an increased vulnerability to violence victimization, which speaks to the need for interventions, and proactive prevention strategies. PMID:29887806
Antagonism between abscisic acid and gibberellins is partially mediated by ascorbic acid during seed germination in rice.

PubMed

Ye, Nenghui; Zhang, Jianhua

2012-05-01

The antagonism between abscisic acid (ABA) and gibberellin (GA) plays a key role in controlling seed germination, but the mechanism of antagonism during this process is not known. In the associated study, we investigated the relationship among ABA, reactive oxygen species (ROS), ascorbic acid (ASC) and GA during rice seed germination. ROS production is reduced by ABA, which hence results in decreasing ASC accumulation during imbibition. GA accumulation was also suppressed by a reduced ROS and ASC level, whereas application of exogenous ASC can partially rescue seed germination from ABA treatment. Further results show that production of ASC, which acts as a substrate in GA biosynthesis, was significantly inhibited by lycorine which thus suppressed the accumulation of GA. Consequently, expression of GA biosynthesis genes was suppressed by the low levels of ROS and ASC in ABA-treated seeds. These studies reveal a new role for ASC in mediating the antagonism between ABA and GA during seed germination in rice.
Eagle-eyed visual acuity: an experimental investigation of enhanced perception in autism.

PubMed

Ashwin, Emma; Ashwin, Chris; Rhydderch, Danielle; Howells, Jessica; Baron-Cohen, Simon

2009-01-01

Anecdotal accounts of sensory hypersensitivity in individuals with autism spectrum conditions (ASC) have been noted since the first reports of the condition. Over time, empirical evidence has supported the notion that those with ASC have superior visual abilities compared with control subjects. However, it remains unclear whether these abilities are specifically the result of differences in sensory thresholds (low-level processing), rather than higher-level cognitive processes. This study investigates visual threshold in n = 15 individuals with ASC and n = 15 individuals without ASC, using a standardized optometric test, the Freiburg Visual Acuity and Contrast Test, to investigate basic low-level visual acuity. Individuals with ASC have significantly better visual acuity (20:7) compared with control subjects (20:13)-acuity so superior that it lies in the region reported for birds of prey. The results of this study suggest that inclusion of sensory hypersensitivity in the diagnostic criteria for ASC may be warranted and that basic standardized tests of sensory thresholds may inform causal theories of ASC.
42 CFR 416.43 - Conditions for coverage-Quality assessment and performance improvement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... and changes in its patient care. (c) Standard: Program activities. (1) The ASC must set priorities for... by the ASC. (2) Addresses the ASC's priorities and that all improvements are evaluated for...
CD54+ rabbit adipose-derived stem cells overexpressing HIF-1α facilitate vascularized fat flap regeneration

PubMed Central

Liang, Zhi-Jie; Huang, Min-Hong; Peng, Qi-Liu; Zou, Dong-Hua; Gu, Rong-He; Xu, Fang-Tian; Gao, Hui; Chen, Zhen-Dong; Chi, Guang-Yi; Wei, Zhong-Heng; Chen, Li; Li, Hong-Mian

2017-01-01

Fat flap transplantation is frequently performed in patients suffering from soft tissue defects resulting from disease or trauma. This study explored the feasibility of constructing vascularized fat flaps using rabbit adipose-derived stem cells (rASCs) and collagen scaffolds in a rabbit model. We evaluated rASCs proliferation, paracrine function, adipogenesis, vascularization, and CD54 expression, with or without HIF-1α transfection in vitro and in vivo. We observed that adipogenic differentiation potential was greater in rASCs with high CD54 expression (CD54+rASCs) than in those with low expression (CD54–rASCs), both in vitro and in vivo. HIF-1α overexpression not only augmented this effect, but also enhanced cell proliferation and paracrine function in vitro. We also demonstrated that HIF-1α-transfected CD54+rASCs showed enhanced paracrine function and adipogenic capacity, and that paracrine function increases expression of angiogenesis-related markers. Thus, CD54+rASCs overexpressing HIF-1α enhanced large volume vascularized fat flap regeneration in rabbits, suggesting CD54 may be an ideal candidate marker for ASCs adipogenic differentiation. PMID:28423354
Adipose Tissue-Derived Mesenchymal Stem Cells Attenuate Staphylococcal Enterotoxin A-Induced Toxic Shock

PubMed Central

Asano, Krisana; Yoshimura, Sayuri

2015-01-01

Adipose tissue-derived stem cells (ASCs), which are mesenchymal stromal cells isolated from adipose tissues, exhibit immunomodulatory effects that are promising for several applications, including the therapeutics of inflammatory diseases. In the present study, the effect of ASCs on bacterial toxin-induced inflammation was investigated. Intraperitoneal administration of ASCs rescued mice from lethal shock induced by staphylococcal enterotoxin A (SEA) potentiated with lipopolysaccharide. In the sera and/or spleens of mice administered ASCs, the production of proinflammatory cytokines, including interferon gamma, tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-2 was reduced. By quantitative real-time PCR, the expression of Foxp3 in the mice administered ASCs was not altered. On the other hand, the expression of IL-12 receptor and STAT4 was decreased with ASC administration. These results imply that the effect of ASCs is not involved in the lineage of regulatory T cells but that these cells may modulate TH1 differentiation. This information provides evidence that ASCs have properties that are effective to attenuate SEA-induced toxic shock and should prompt further exploration on other inflammatory diseases caused by bacterial toxins or bacterial infections. PMID:26099581
Medicare Program: Hospital Outpatient Prospective Payment and Ambulatory Surgical Center Payment Systems and Quality Reporting Programs; Organ Procurement Organization Reporting and Communication; Transplant Outcome Measures and Documentation Requirements; Electronic Health Record (EHR) Incentive Programs; Payment to Nonexcepted Off-Campus Provider-Based Department of a Hospital; Hospital Value-Based Purchasing (VBP) Program; Establishment of Payment Rates Under the Medicare Physician Fee Schedule for Nonexcepted Items and Services Furnished by an Off-Campus Provider-Based Department of a Hospital. Final rule with comment period and interim final rule with comment period.

PubMed

2016-11-14

This final rule with comment period revises the Medicare hospital outpatient prospective payment system (OPPS) and the Medicare ambulatory surgical center (ASC) payment system for CY 2017 to implement applicable statutory requirements and changes arising from our continuing experience with these systems. In this final rule with comment period, we describe the changes to the amounts and factors used to determine the payment rates for Medicare services paid under the OPPS and those paid under the ASC payment system. In addition, this final rule with comment period updates and refines the requirements for the Hospital Outpatient Quality Reporting (OQR) Program and the ASC Quality Reporting (ASCQR) Program. Further, in this final rule with comment period, we are making changes to tolerance thresholds for clinical outcomes for solid organ transplant programs; to Organ Procurement Organizations (OPOs) definitions, outcome measures, and organ transport documentation; and to the Medicare and Medicaid Electronic Health Record Incentive Programs. We also are removing the HCAHPS Pain Management dimension from the Hospital Value-Based Purchasing (VBP) Program. In addition, we are implementing section 603 of the Bipartisan Budget Act of 2015 relating to payment for certain items and services furnished by certain off-campus provider-based departments of a provider. In this document, we also are issuing an interim final rule with comment period to establish the Medicare Physician Fee Schedule payment rates for the nonexcepted items and services billed by a nonexcepted off-campus provider-based department of a hospital in accordance with the provisions of section 603.
77 FR 9687 - Endangered Species; Receipt of Applications for Permit

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-17

.... Permit Applications A. Endangered Species Applicant: Point Defiance Zoo & Aquarium, Tacoma, WA; PRT... activities to be conducted by the applicant over a 5-year period. Applicant: Triple D Game Farm Inc... a 5-year period. Applicant: Buck Valley Ranch, LLC, Center Point, TX; PRT-65292A The applicant...

Some links on this page may take you to non-federal websites. Their policies may differ from this site.