Sample records for tamias sibiricus barberi

  1. Introduced Siberian chipmunks (Tamias sibiricus barberi) contribute more to lyme borreliosis risk than native reservoir rodents.

    PubMed

    Marsot, Maud; Chapuis, Jean-Louis; Gasqui, Patrick; Dozières, Anne; Masséglia, Sébastien; Pisanu, Benoit; Ferquel, Elisabeth; Vourc'h, Gwenaël

    2013-01-01

    The variation of the composition in species of host communities can modify the risk of disease transmission. In particular, the introduction of a new host species can increase health threats by adding a new reservoir and/or by amplifying the circulation of either exotic or native pathogens. Lyme borreliosis is a multi-host vector-borne disease caused by bacteria belonging to the Borrelia burgdorferi sensu lato complex. It is transmitted by the bite of hard ticks, especially Ixodes ricinus in Europe. Previous studies showed that the Siberian chipmunk, Tamias sibiricus barberi, an introduced ground squirrel in the Forest of Sénart (near Paris, France) was highly infested by I. ricinus, and consequently infected by B. burgdorferi sl. An index of the contribution of chipmunks to the density of infected questing nymphs on the vegetation (i.e., the acarological risk for humans) was compared to that of bank voles (Myodes glareolus) and of wood mice (Apodemus sylvaticus), two known native and sympatric competent reservoir hosts. Chipmunks produced nearly 8.5 times more infected questing nymphs than voles and mice. Furthermore, they contribute to a higher diversity of B. burgdorferi sl genospecies (B. afzelii, B. burgdorferi sensu stricto and B. garinii). The contribution of chipmunks varied between years and seasons, according to tick availability. As T. s. barberi must be a competent reservoir, it should amplify B. burgdorferi sl infection, hence increasing the risk of Lyme borreliosis in humans.

  2. Introduced Siberian Chipmunks (Tamias sibiricus barberi) Contribute More to Lyme Borreliosis Risk than Native Reservoir Rodents

    PubMed Central

    Marsot, Maud; Chapuis, Jean-Louis; Gasqui, Patrick; Dozières, Anne; Masséglia, Sébastien; Pisanu, Benoit; Ferquel, Elisabeth; Vourc’h, Gwenaël

    2013-01-01

    The variation of the composition in species of host communities can modify the risk of disease transmission. In particular, the introduction of a new host species can increase health threats by adding a new reservoir and/or by amplifying the circulation of either exotic or native pathogens. Lyme borreliosis is a multi-host vector-borne disease caused by bacteria belonging to the Borrelia burgdorferi sensu lato complex. It is transmitted by the bite of hard ticks, especially Ixodes ricinus in Europe. Previous studies showed that the Siberian chipmunk, Tamias sibiricus barberi, an introduced ground squirrel in the Forest of Sénart (near Paris, France) was highly infested by I. ricinus, and consequently infected by B. burgdorferi sl. An index of the contribution of chipmunks to the density of infected questing nymphs on the vegetation (i.e., the acarological risk for humans) was compared to that of bank voles (Myodes glareolus) and of wood mice (Apodemus sylvaticus), two known native and sympatric competent reservoir hosts. Chipmunks produced nearly 8.5 times more infected questing nymphs than voles and mice. Furthermore, they contribute to a higher diversity of B. burgdorferi sl genospecies (B. afzelii, B. burgdorferi sensu stricto and B. garinii). The contribution of chipmunks varied between years and seasons, according to tick availability. As T. s. barberi must be a competent reservoir, it should amplify B. burgdorferi sl infection, hence increasing the risk of Lyme borreliosis in humans. PMID:23383170

  3. Visual landmark-directed scatter-hoarding of Siberian chipmunks Tamias sibiricus.

    PubMed

    Zhang, Dongyuan; Li, Jia; Wang, Zhenyu; Yi, Xianfeng

    2016-05-01

    Spatial memory of cached food items plays an important role in cache recovery by scatter-hoarding animals. However, whether scatter-hoarding animals intentionally select cache sites with respect to visual landmarks in the environment and then rely on them to recover their cached seeds for later use has not been extensively explored. Furthermore, there is a lack of evidence on whether there are sex differences in visual landmark-based food-hoarding behaviors in small rodents even though male and female animals exhibit different spatial abilities. In the present study, we used a scatter-hoarding animal, the Siberian chipmunk, Tamias sibiricus to explore these questions in semi-natural enclosures. Our results showed that T. sibiricus preferred to establish caches in the shallow pits labeled with visual landmarks (branches of Pinus sylvestris, leaves of Athyrium brevifrons and PVC tubes). In addition, visual landmarks of P. sylvestris facilitated cache recovery by T. sibiricus. We also found significant sex differences in visual landmark-based food-hoarding strategies in Siberian chipmunks. Males, rather than females, chipmunks tended to establish their caches with respect to the visual landmarks. Our studies show that T. sibiricus rely on visual landmarks to establish and recover their caches, and that sex differences exist in visual landmark-based food hoarding in Siberian chipmunks. © 2015 International Society of Zoological Sciences, Institute of Zoology/Chinese Academy of Sciences and John Wiley & Sons Australia, Ltd.

  4. Mitochondrial cytochrome b sequence variations and population structure of Siberian chipmunk (Tamias sibiricus) in Northeastern Asia and population substructure in South Korea.

    PubMed

    Lee, Mu-Yeong; Lissovsky, Andrey A; Park, Sun-Kyung; Obolenskaya, Ekaterina V; Dokuchaev, Nikolay E; Zhang, Ya-Ping; Yu, Li; Kim, Young-Jun; Voloshina, Inna; Myslenkov, Alexander; Choi, Tae-Young; Min, Mi-Sook; Lee, Hang

    2008-12-31

    Twenty-five chipmunk species occur in the world, of which only the Siberian chipmunk, Tamias sibiricus, inhabits Asia. To investigate mitochondrial cytochrome b sequence variations and population structure of the Siberian chipmunk in northeastern Asia, we examined mitochondrial cytochrome b sequences (1140 bp) from 3 countries. Analyses of 41 individuals from South Korea and 33 individuals from Russia and northeast China resulted in 37 haplotypes and 27 haplotypes, respectively. There were no shared haplotypes between South Korea and Russia--northeast China. Phylogenetic trees and network analysis showed 2 major maternal lineages for haplotypes, referred to as the S and R lineages. Haplotype grouping in each cluster was nearly coincident with its geographic affinity. In particular, 3 distinct groups were found that mostly clustered in the northern, central and southern parts of South Korea. Nucleotide diversity of the S lineage was twice that of lineage R. The divergence between S and R lineages was estimated to be 2.98-0.98 Myr. During the ice age, there may have been at least 2 refuges in South Korea and Russia--northeast China. The sequence variation between the S and R lineages was 11.3% (K2P), which is indicative of specific recognition in rodents. These results suggest that T. sibiricus from South Korea could be considered a separate species. However, additional information, such as details of distribution, nuclear genes data or morphology, is required to strengthen this hypothesis.

  5. Why do Siberian chipmunks Tamias sibiricus (Sciuridae) introduced in French forests acquired so few intestinal helminth species from native sympatric murids?

    PubMed

    Pisanu, Benoît; Lebailleux, Lise; Chapuis, Jean-Louis

    2009-02-01

    A lack of newly acquired species partly explains why introduced host species have poor specific parasite diversity. The intestinal helminth community from two native Murid host species, wood mice Apodemus sylvaticus [Murinae] (n=40), bank voles Clethrionomys glareolus [Arvicolinae] (n=42), and an introduced Sciurid, the Siberian chipmunk Tamias sibiricus (n=42), dominant in the rodent communities, was studied from three woody areas in the Ile-de-France region. Native gastrointestinal helminth fauna from mice and voles was formed by 12 taxa: ten nematodes, Aonchotheca murissylvatici, Aonchotheca annulosa, Aspiculuris tetraptera, Eucoleus sp., Heligmosomoides glareoli, Heligmosomoides polygyrus, Mastophorus muris, Syphacia frederici, Syphacia stroma, Trichuris muris, a Cestode and a Trematode. Two helminth taxa were imported by chipmunks from eastern Asia: Brevistriata skrjabini and Strongyloides callosciureus. Only A. annulosa was transferred to chipmunks from the native small rodent community. None of the 82 native murids harbored chipmunk helminths. The developmental ability of helminth according to host phylogenetic relatedness was the main driving force explaining the species composition of the helminth community between these sympatric native and introduced hosts.

  6. Borrelia burgdorferi Sensu Lato in Siberian chipmunks (Tamias sibiricus) introduced in suburban forests in France.

    PubMed

    Vourc'h, Gwenaël; Marmet, Julie; Chassagne, Michelle; Bord, Séverine; Chapuis, Jean-Louis

    2007-01-01

    Numerous vertebrate reservoirs have been described for Borrelia burgdorferi sensu lato (sl), which includes the etiological agents of Lyme Borreliosis (LB). The Siberian chipmunk (Tamias sibiricus) is a rodent originating from Asia, where it is suspected to be a B. burgdorferi reservoir. It has been intentionally released into the wild in Europe since the 1970s, but has not yet been subject to any study regarding its association with the LB agent. In this paper we studied Siberian chipmunk infestation with the LB vector (Ixodes ricinus) and infection prevalence by LB spirochetes in a suburban introduced population. We compared these findings with known competent reservoir hosts, the bank vole (Myodes [clethrionomys] glareolus) and wood mouse (Apodemus sylvaticus). All Siberian chipmunks were infested with larvae and larval abundance was higher in this species (mean number of larvae [95% Confidence Interval]: 73.5 [46.0, 117.2]) than in the two other rodent species (bank voles: 4.4 [3.0, 6.3] and wood mice: 10.2 [4.9, 21.2]). Significant factors affecting abundance of larvae were host species and sampling season. Nymphs were most prevalent on chipmunks (86.2%, mean: 5.1 [3.3, 8.0]), one vole carried only two nymphs, and none of the mice had any nymphs. Nymph abundance in chipmunks was affected by sampling season and sex. Furthermore, the infection prevalence of B. burgdorferi sl in the Siberian chipmunk was the highest (33.3%) and predominantly of B. afzelii. The infection prevalence was 14.1% in bank voles, but no wood mouse was found to be infected. Our results suggest that the Siberian chipmunk may be an important reservoir host for LB.

  7. [Fleas community in introduced Siberian chipmunks (Tamias sibiricus Laxmann) in Forest of Sénart, France].

    PubMed

    Pisanu, B; Marmet, J; Beaucournu, J C; Chapuis, J L

    2008-03-01

    We examined the fleas community in an introduced population of Siberian chipmunks, Tamias sibiricus, between 2005 and 2007, in the Forest of Sénart (Essonne, France). We collected and identified 383 fleas on 463 chipmunks (total: 1,891 captures on 471 chipmunks). In 2005, 120 fleas were also collected on 65 bank voles, Clethrionomys glareolus, and on 25 wood mice, Apodemus sylvaticus, trapped within the same area. Ceratophyllus sciurorum sciurorum formed 73.6% of the chipmunks' flea community, with an annual prevalence (P) ranging between 8 and 13% and a mean intensity (I) ranging between 1.1 and 1.6 fleas per individual. Among the six other species infecting this Sciurid, Ctenophthalmus agyrtes impavidus constituted 17.2% (P: 1.6-2.2%; I: 1.1-2.6), and Megabothris turbidus 8.1% (P: 0.8-1.9%; I: 1.0-1.4) of the flea community, respectively. These last two species represented respectively 60.8% and 36.6% of the flea community on the bank vole and the wood mouse. Originated from Asia, chipmunks did not import any flea species to Sénart, probably because they were used as pets before their release in the wild. Abundance in C. s. sciurorum increased with adult chipmunk density and with juvenile density in summer. On adult chipmunks C. s. sciurorum tented to decrease with increasing abundance of red squirrels (Sciurus vulgaris]. Moreover, the two other flea species mainly infected young chipmunks during the fall, and their number was not related to chipmunk density. However, the distribution of species within the flea community became more balanced with increase juvenile chipmunk density. Overall, these results indicate that the close phyletic relationship between chipmunks and red squirrels contributed in the acquisition and the spread of fleas by chipmunks. Primary and secondary hosts densities, their habitat use, and more specifically burrowing activities and tree canopy use, also played a role in the spread of fleas on chipmunks.

  8. Attraction of Diabrotica barberi Smith and Lawrence (Coleoptera: Chrysomelidae) to eugenol-baited traps in soybean

    USDA-ARS?s Scientific Manuscript database

    Diabrotica barberi Smith and Lawrence (the northern corn rootworm) is a native North American leaf beetle and a major pest of corn. However, adult D. barberi forage in various habitats outside of corn, including soybean, roadside vegetation, and prairie. Eugenol is a common floral volatile that ha...

  9. Extremely Low Genetic Diversity Indicating the Endangered Status of Ranodon sibiricus (Amphibia: Caudata) and Implications for Phylogeography

    PubMed Central

    Wang, Xiu-Ling; Sun, Jian-Yun; Xue, Yan; Zhang, Peng; Zhou, Hui; Qu, Liang-Hu

    2012-01-01

    Background The Siberian salamander (Ranodon sibiricus), distributed in geographically isolated areas of Central Asia, is an ideal alpine species for studies of conservation and phylogeography. However, there are few data regarding the genetic diversity in R. sibiricus populations. Methodology/Principal Findings We used two genetic markers (mtDNA and microsatellites) to survey all six populations of R. sibiricus in China. Both of the markers revealed extreme genetic uniformity among these populations. There were only three haplotypes in the mtDNA, and the overall nucleotide diversity in the mtDNA was 0.00064, ranging from 0.00000 to 0.00091 for the six populations. Although we recovered 70 sequences containing microsatellite repeats, there were only two loci that displayed polymorphism. We used the approximate Bayesian computation (ABC) method to study the demographic history of the populations. This analysis suggested that the extant populations diverged from the ancestral population approximately 120 years ago and that the historical population size was much larger than the present population size; i.e., R. sibiricus has experienced dramatic population declines. Conclusion/Significance Our findings suggest that the genetic diversity in the R. sibiricus populations is the lowest among all investigated amphibians. We conclude that the isolation of R. sibiricus populations occurred recently and was a result of recent human activity and/or climatic changes. The Pleistocene glaciation oscillations may have facilitated intraspecies genetic homogeneity rather than enhanced divergence. A low genomic evolutionary rate and elevated inbreeding frequency may have also contributed to the low genetic variation observed in this species. Our findings indicate the urgency of implementing a protection plan for this endangered species. PMID:22428037

  10. First nearctic records for Orius (Dimorphella) sibiricus Wagner (Hemiptera: Heteroptera: Anthocoridae), a Eurasian steppe inhabitant

    USDA-ARS?s Scientific Manuscript database

    Orius sibiricus Wagner, a dark-colored minute pirate bug widespread in the Eurasian Steppe, is recorded from sites near the Yukon River in Yukon, Canada. This species is distinguished from the melanic phenotype of Orius diespeter Herring by the more deeply and uniformly punctured dorsum, the subangu...

  11. Effects of tannins on population dynamics of sympatric seed-eating rodents: the potential role of gut tannin-degrading bacteria.

    PubMed

    Zhang, Yihao; Bartlow, Andrew W; Wang, Zhenyu; Yi, Xianfeng

    2018-05-07

    Chemical compounds in seeds exert negative and even lethal effects on seed-consuming animals. Tannin-degrading bacteria in the guts of small mammals have been associated with the ability to digest seeds high in tannins. At the population level, it is not known if tannins influence rodent species differently according to the composition of their gut microbiota. Here, we test the hypothesis that sympatric tree species with different tannins exert contrasting effects on population fluctuations of seed-eating rodents. We collected a 10-year dataset of seed crops and rodent population sizes and sequenced 16S rRNA of gut microbes. The abundance of Apodemus peninsulae was not correlated with seed crop of either high-tannin Quercus mongolica or low-tannin Corylus mandshurica, but positively correlated with their total seed crops. Abundance of Tamias sibiricus was negatively correlated with seed crop of Q. mongolica but positively correlated with C. mandshurica. Body masses of A. peninsulae and T. sibiricus decreased when given high-tannin food; however, only the survival of T. sibiricus was reduced. The abundance of microbial genus Lactobacillus exhibiting potential tannin-degrading activity was significantly higher in A. peninsulae than in T. sibiricus. Our results suggest that masting tree species with different tannin concentrations may differentially influence population fluctuations of seed predators hosting different gut microbial communities. Although the conclusion is based on just correlational analysis of a short time-series, seeds with different chemical composition may influence rodent populations differently. Future work should examine these questions further to understand the complex interactions among seeds, gut microbes, and animal populations.

  12. Forest-floor disturbance reduces chipmunk (Tamias spp.) abundance two years after variable-retention harvest of Pacific Northwestern forests

    Treesearch

    Randall J. Wilk; Timothy B. Harrington; Robert A. Gitzen; Chris C. Maguire

    2015-01-01

    We evaluated the two-year effects of variable-retention harvest on chipmunk (Tamias spp.) abundance (N^) and habitat in mature coniferous forests in western Oregon and Washington because wildlife responses to density/pattern of retained trees remain largely unknown. In a randomized complete-block design, six...

  13. Control of Larval Northern Corn Rootworm. (Diabrotica barberi) with Two Steinernematid Nematode Species

    PubMed Central

    Thurston, G. S.; Yule, W. N.

    1990-01-01

    The entomogenous nematodes Steinerema feltiae and S. bibionis did not penetrate the roots of corn, Zea mays, to infect larval northern corn rootworm (NCR), Diabrotica barberi, feeding within. Laboratory bioassays against first instar NCR indicated that S. feltiae, Mexican strain (LD₅₀ = 49 nematodes/insect) is more virulent than S. bibionis (LD₅₀ = 100). Numbers of NCR larvae in a grain corn crop were reduced by both nematode species applied at corn seeding time at the rate of 10,000 infective-stage juveniles per linear meter of corn row. The chemical insecticide fonofos provided significantly better control than either nematode species. PMID:19287699

  14. Influence of canopy closure and shrub coverage on travel along coarse woody debris by Eastern chipmunks (Tamias striatus)

    Treesearch

    Patrick A. Zollner; Kevin J. Crane

    2003-01-01

    We investigated relationships between canopy closure, shrub cover and the use of coarse woody debris as a travel path by eastern chipmunks (Tamias striatus) in the north central United States. Fine scale movements of chipmunks were followed with tracking spools and the percentage of each movement path directly along coarse woody debris was recorded...

  15. Transformed Root Extract of Leonurus sibiricus Induces Apoptosis through Intrinsic and Extrinsic Pathways in Various Grades of Human Glioma Cells.

    PubMed

    Sitarek, Przemysław; Skała, Ewa; Toma, Monika; Wielanek, Marzena; Szemraj, Janusz; Skorski, Tomasz; Białas, Adam J; Sakowicz, Tomasz; Kowalczyk, Tomasz; Radek, Maciej; Wysokińska, Halina; Śliwiński, Tomasz

    2017-07-01

    This study determines the influence of transformed root (TR) extract of Leonurus sibiricus L. on various grades (I-III) of human glioma cells derived from patients. This plant occurs in southern Asia and Siberia and is widely used as a medicinal plant with various biological activities. Chromatographic profile of TR extract have revealed the presence of various polyphenolic compounds (4-hydroxybenzoic acid, gentisic acid, vanilic acid, 1,3-dicaffeoylquinic acid, α-resorcylic acid). We found TR root extract to have antiproliferative activity on glioma cells after 24 h of treatment. TR root extract induces apoptosis on various grades (I-III) of human glioma cells by the generation of reactive oxygen species (ROS) along with concurrent loss of mitochondrial membrane potential, enhanced S and G2/M phases of the cell cycle, and altered mRNA levels of Bax, Bcl-2, p53, Cas-3, Cas-8 and Cas-9 factors involved in apoptosis. This work for the first time demonstrate that TR extract from L. sibiricus root has the potential to activate apoptosis in grade I-III human glioma cells through the intrinsic and extrinsic pathways.

  16. Catalytic effect of Ag⁺ on arsenic bioleaching from orpiment (As₂S₃) in batch tests with Acidithiobacillus ferrooxidans and Sulfobacillus sibiricus.

    PubMed

    Zhang, Guangji; Chao, Xingwu; Guo, Pei; Cao, Junya; Yang, Chao

    2015-01-01

    Orpiment is one of the major arsenic sulfide minerals which commonly occurs in the gold mine environment and the weathering of this mineral can lead to the contamination of arsenic. In this study, chemical leaching experiments using 10g/L Fe(3+) at 35°C and 50°C were carried out and the results show that orpiment can be leached by Fe(3+) and the leaching rate of orpiment was significantly enhanced in the presence of Ag(+). The bioleaching experiments with mesophilic bacteria Acidithiobacillus ferrooxidans and moderate thermophilic bacteria Sulfobacillus sibiricus were carried out, showing that these two strains can survive in the mineral pulp and oxidize Fe(2+) to regenerate Fe(3+). Based on above results, it is believed that the leaching action of the acidic mining drainage by some bacteria can lead to the release of arsenic from orpiment. Different performances of At. ferrooxidans and S. sibiricus in the tests suggest they follow two different mechanisms and this point of view is further confirmed based on analyses of the composition and morphology of the mineral residue by SEM and EDS. Copyright © 2014 Elsevier B.V. All rights reserved.

  17. Infection of Ixodes ricinus by Borrelia burgdorferi sensu lato in peri-urban forests of France

    PubMed Central

    Perthame, Emeline; Sertour, Natacha; Garnier, Martine; Godard, Vincent

    2017-01-01

    Lyme borreliosis is the most common tick-borne disease in the northern hemisphere. In Europe, it is transmitted by Ixodes ticks that carry bacteria belonging to the Borrelia burgdorferi sensu lato complex. The objective of this work was to explore eco-epidemiological factors of Lyme borreliosis in peri-urban forests of France (Sénart, Notre-Dame and Rambouillet). We investigated whether the introduction of Tamias sibiricus in Sénart could alter the density of infected ticks. Moreover, the density and tick infection were investigated according to the tree species found in various patches of Sénart forest. For this purpose, ticks were sampled during 3 years. In the Sénart forest, the density of nymph and adult ticks showed no significant difference between 2008, 2009 and 2011. The nymph density varied significantly as a function of the month of collection. Regarding the nymphs, a higher rate of infection and infected density were found in 2009. Plots with chipmunks (C) presented a lower density of both nymphs and adult ticks than plots without chipmunks (NC) did. A higher rate of infection of nymphs with Borrelia was seen in C plots. The prevalence of the various species of Borrelia was also found to vary between C and NC plots with the year of the collect. The presence of chestnut trees positively influenced the density of both nymphs and adults. The infected nymph density showed a significant difference depending on the peri-urban forest studied, Sénart being higher than Rambouillet. The prevalence of Borrelia species also differed between the various forests studied. Concerning the putative role that Tamias sibiricus may play in the transmission of Borrelia, our results suggest that its presence is correlated with a higher rate of infection of questing ticks by Borrelia genospecies and if its population increases, it could play a significant role in the risk of transmission of Lyme borreliosis. PMID:28846709

  18. Infection of Ixodes ricinus by Borrelia burgdorferi sensu lato in peri-urban forests of France.

    PubMed

    Marchant, Axelle; Le Coupanec, Alain; Joly, Claire; Perthame, Emeline; Sertour, Natacha; Garnier, Martine; Godard, Vincent; Ferquel, Elisabeth; Choumet, Valerie

    2017-01-01

    Lyme borreliosis is the most common tick-borne disease in the northern hemisphere. In Europe, it is transmitted by Ixodes ticks that carry bacteria belonging to the Borrelia burgdorferi sensu lato complex. The objective of this work was to explore eco-epidemiological factors of Lyme borreliosis in peri-urban forests of France (Sénart, Notre-Dame and Rambouillet). We investigated whether the introduction of Tamias sibiricus in Sénart could alter the density of infected ticks. Moreover, the density and tick infection were investigated according to the tree species found in various patches of Sénart forest. For this purpose, ticks were sampled during 3 years. In the Sénart forest, the density of nymph and adult ticks showed no significant difference between 2008, 2009 and 2011. The nymph density varied significantly as a function of the month of collection. Regarding the nymphs, a higher rate of infection and infected density were found in 2009. Plots with chipmunks (C) presented a lower density of both nymphs and adult ticks than plots without chipmunks (NC) did. A higher rate of infection of nymphs with Borrelia was seen in C plots. The prevalence of the various species of Borrelia was also found to vary between C and NC plots with the year of the collect. The presence of chestnut trees positively influenced the density of both nymphs and adults. The infected nymph density showed a significant difference depending on the peri-urban forest studied, Sénart being higher than Rambouillet. The prevalence of Borrelia species also differed between the various forests studied. Concerning the putative role that Tamias sibiricus may play in the transmission of Borrelia, our results suggest that its presence is correlated with a higher rate of infection of questing ticks by Borrelia genospecies and if its population increases, it could play a significant role in the risk of transmission of Lyme borreliosis.

  19. The ethanol extract of Leonurus sibiricus L. induces antioxidant, antinociceptive and topical anti-inflammatory effects.

    PubMed

    Oliveira, Alan Santos; Cercato, Luana Mendonça; de Santana Souza, Marília Trindade; Melo, Allan John de Oliveira; Lima, Bruno Dos Santos; Duarte, Marcelo Cavalcante; Araujo, Adriano Antunes de Souza; de Oliveira E Silva, Ana Mara; Camargo, Enilton Aparecido

    2017-07-12

    Leonurus sibiricus L. (Lamiaceae), popularly known as motherwort, or "erva-de-macaé" or "rubim" in Brazil, is a plant used for the treatment of inflammatory conditions, but few studies have evaluated this anti-inflammatory activity or other activities that may be relevant. This study was undertaken to investigate the antioxidant, antinociceptive and topical anti-inflammatory effects of the ethanol extract of L. sibiricus (EELs). Chromatographic analysis, determination of total phenolic and flavonoid contents and in vitro antioxidant assays were performed, while the formalin test and ear inflammation induced by 12-0-tetradecanoylphorbol-13-acetate (TPA) were performed in mice. We observed that total phenolic and flavonoids content in EELs were respectively 60.1mg of gallic acid equivalent/g of extract and 15.4mg of catechin equivalent/g of extract. Chlorogenic, caffeic, p-coumaric and ferulic acids, as well as quercetin were identified in EELs. This extract also led to the consumption of the radicals 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and nitric oxide, increased the ferric reducing/antioxidant power (FRAP) and inhibited the spontaneous or FeSO 4 -induced in vitro lipid peroxidation. In the formalin test, oral pretreatment with EELs (400mg/kg) reduced (p<0.001) the licking/biting time in the second phase, but not in the first phase. In the ear inflammation induced by TPA, the concomitant topical administration of EELs (0.3-3mg/ear) significantly reduced the edema, myeloperoxidase activity, levels of tumoral necrosis factor-α and interleukin-1β and lipoperoxidation, as well as increased FRAP in ear tissue when compared to vehicle-treated ears. These results indicate that EELs has antioxidant, antinociceptive and topical anti-inflammatory activities, supporting the use of this plant in folk medicine. Copyright © 2017 Elsevier Ireland Ltd. All rights reserved.

  20. A long-living species of the hydrophiloid beetles: Helophorus sibiricus from the early Miocene deposits of Kartashevo (Siberia, Russia)

    PubMed Central

    Fikáček, Martin; Prokin, Alexander; Angus, Robert B.

    2011-01-01

    Abstract The recent hydrophiloid species Helophorus (Gephelophorus) sibiricus (Motschulsky, 1860) is recorded from the early Miocene deposits of Kartashevo assigned to the Ombinsk Formation. A detailed comparison with recent specimens allowed a confident identification of the fossil specimen, which is therefore the oldest record of a recent species for the Hydrophiloidea. The paleodistribution as well as recent distribution of the species is summarized, and the relevance of the fossil is discussed. In addition, the complex geological settings of the Kartashevo area are briefly summarized. PMID:22259280

  1. Antibacterial, Anti-Inflammatory, Antioxidant, and Antiproliferative Properties of Essential Oils from Hairy and Normal Roots of Leonurus sibiricus L. and Their Chemical Composition.

    PubMed

    Sitarek, Przemysław; Rijo, Patricia; Garcia, Catarina; Skała, Ewa; Kalemba, Danuta; Białas, Adam J; Szemraj, Janusz; Pytel, Dariusz; Toma, Monika; Wysokińska, Halina; Śliwiński, Tomasz

    2017-01-01

    Essential oils obtained from the NR (normal roots) and HR (hairy roots) of the medicinal plant Leonurus sibiricus root were used in this study. The essential oil compositions were detected by GC-MS. Eighty-five components were identified in total. Seventy components were identified for NR essential oil. The major constituents in NR essential oil were β -selinene (9.9%), selina-4,7-diene (9.7%), (E) - β -caryophyllene (7.3%),myli-4(15)-ene (6.4%), and guaia-1(10),11-diene (5.9%). Sixty-seven components were identified in HR essential oil, the main constituents being (E) - β -caryophyllene (22.6%), and germacrene D (19.8%). The essential oils were tested for cytotoxic effect, antimicrobial, anti-inflammatory, and antioxidant activities. Both essential oils showed activity against grade IV glioma cell lines (IC 50 = 400  μ g/mL), antimicrobial (MIC and MFC values of 2500 to 125  μ g/mL), and anti-inflammatory (decreased level of IL-1 β , IL-6, TNF- α , and IFN- γ in LPS-stimulated cells).The essential oils exhibited moderate antioxidant activity in ABTS (EC 50 = 98 and 88  μ g/mL) assay. This is the first study to examine composition of the essential oils and their antimicrobial, antioxidant, antiproliferative, and anti-inflammatory activities. The results indicate that essential oils form L. sibiricus root may be used in future as an alternative to synthetic antimicrobial agents with potential application in the food and pharmaceutical industries.

  2. Divergence with gene flow within the recent chipmunk radiation (Tamias)

    PubMed Central

    Sullivan, J; Demboski, J R; Bell, K C; Hird, S; Sarver, B; Reid, N; Good, J M

    2014-01-01

    Increasing data have supported the importance of divergence with gene flow (DGF) in the generation of biological diversity. In such cases, lineage divergence occurs on a shorter timescale than does the completion of reproductive isolation. Although it is critical to explore the mechanisms driving divergence and preventing homogenization by hybridization, it is equally important to document cases of DGF in nature. Here we synthesize data that have accumulated over the last dozen or so years on DGF in the chipmunk (Tamias) radiation with new data that quantify very high rates of mitochondrial DNA (mtDNA) introgression among para- and sympatric species in the T. quadrivittatus group in the central and southern Rocky Mountains. These new data (188 cytochrome b sequences) bring the total number of sequences up to 1871; roughly 16% (298) of the chipmunks we have sequenced exhibit introgressed mtDNA. This includes ongoing introgression between subspecies and between both closely related and distantly related taxa. In addition, we have identified several taxa that are apparently fixed for ancient introgressions and in which there is no evidence of ongoing introgression. A recurrent observation is that these introgressions occur between ecologically and morphologically diverged, sometimes non-sister taxa that engage in well-documented niche partitioning. Thus, the chipmunk radiation in western North America represents an excellent mammalian example of speciation in the face of recurrent gene flow among lineages and where biogeography, habitat differentiation and mating systems suggest important roles for both ecological and sexual selection. PMID:24781803

  3. Antibacterial, Anti-Inflammatory, Antioxidant, and Antiproliferative Properties of Essential Oils from Hairy and Normal Roots of Leonurus sibiricus L. and Their Chemical Composition

    PubMed Central

    Rijo, Patricia; Garcia, Catarina; Kalemba, Danuta; Szemraj, Janusz; Pytel, Dariusz; Toma, Monika; Śliwiński, Tomasz

    2017-01-01

    Essential oils obtained from the NR (normal roots) and HR (hairy roots) of the medicinal plant Leonurus sibiricus root were used in this study. The essential oil compositions were detected by GC-MS. Eighty-five components were identified in total. Seventy components were identified for NR essential oil. The major constituents in NR essential oil were β-selinene (9.9%), selina-4,7-diene (9.7%), (E)-β-caryophyllene (7.3%),myli-4(15)-ene (6.4%), and guaia-1(10),11-diene (5.9%). Sixty-seven components were identified in HR essential oil, the main constituents being (E)-β-caryophyllene (22.6%), and germacrene D (19.8%). The essential oils were tested for cytotoxic effect, antimicrobial, anti-inflammatory, and antioxidant activities. Both essential oils showed activity against grade IV glioma cell lines (IC50 = 400 μg/mL), antimicrobial (MIC and MFC values of 2500 to 125 μg/mL), and anti-inflammatory (decreased level of IL-1β, IL-6, TNF-α, and IFN-γ in LPS-stimulated cells).The essential oils exhibited moderate antioxidant activity in ABTS (EC50 = 98 and 88 μg/mL) assay. This is the first study to examine composition of the essential oils and their antimicrobial, antioxidant, antiproliferative, and anti-inflammatory activities. The results indicate that essential oils form L. sibiricus root may be used in future as an alternative to synthetic antimicrobial agents with potential application in the food and pharmaceutical industries. PMID:28191277

  4. Distribution and prevalence of vector-borne diseases in California chipmunks (Tamias spp.)

    PubMed Central

    Roy, Austin N.; Martin, Amanda; Sholty, Kathleen E.; Stephenson, Nicole; Foley, Janet E.

    2017-01-01

    California, with 13 chipmunk (Tamias) species, has more than any other state or country, occupying habitats ranging from chaparral to the high peaks of the Sierra Nevada. Chipmunks host zoonotic pathogens including Yersinia pestis, Anaplasma phagocytophilum, relapsing fever (RF) Borrelia spp., Borrelia burgdorferi, and spotted fever group (SFG) Rickettsia species. Chipmunk species are often not differentiated by public health workers, yet different species utilize different ecological niches and may have intrinsically different capacities for maintaining vector-borne pathogens and infecting vectors. We surveyed over 700 individuals from nine species of chipmunks throughout California for exposure to and infection by Y. pestis, A. phagocytophilum, RF Borrelia spp., Borrelia burgdorferi, and SFG Rickettsia species. DNA of all five pathogens was found and all chipmunks except Merriam’s chipmunk (T. merriami) were PCR-positive for at least one of the pathogens. Anaplasma phagocytophilum was most common (40.0%, 2/5) in Sonoma chipmunks (T. sonomae) from Marin county and B. burgdorferi most common (37.5%, 27/72) in redwood chipmunks (T. ochrogenys) from Mendocino county. RF Borrelia spp. was detected in 2% (6/297) of redwood chipmunks in Mendocino county and 10% (1/10) of both least (T. minimus) and lodgepole (T. speciosus) chipmunks in the western Sierra. Exposure to SFG Rickettsia spp. was found in the Northern Coastal region (Del Norte, Humboldt and Mendocino counties) and in the northern and western Sierra in several species of chipmunks. Y. pestis infection was found only in the western Sierra—in a yellow-pine (T. amoenus) and a long-eared (T. quadrimaculatus) chipmunk. Though more data are needed to thoroughly understand the roles that different chipmunk species play in disease transmission, our findings suggest that some chipmunk species may be more important to the maintenance of vector-borne diseases than others within each geographic area. PMID:29232397

  5. Distribution and prevalence of vector-borne diseases in California chipmunks (Tamias spp.).

    PubMed

    Straub, Mary H; Roy, Austin N; Martin, Amanda; Sholty, Kathleen E; Stephenson, Nicole; Foley, Janet E

    2017-01-01

    California, with 13 chipmunk (Tamias) species, has more than any other state or country, occupying habitats ranging from chaparral to the high peaks of the Sierra Nevada. Chipmunks host zoonotic pathogens including Yersinia pestis, Anaplasma phagocytophilum, relapsing fever (RF) Borrelia spp., Borrelia burgdorferi, and spotted fever group (SFG) Rickettsia species. Chipmunk species are often not differentiated by public health workers, yet different species utilize different ecological niches and may have intrinsically different capacities for maintaining vector-borne pathogens and infecting vectors. We surveyed over 700 individuals from nine species of chipmunks throughout California for exposure to and infection by Y. pestis, A. phagocytophilum, RF Borrelia spp., Borrelia burgdorferi, and SFG Rickettsia species. DNA of all five pathogens was found and all chipmunks except Merriam's chipmunk (T. merriami) were PCR-positive for at least one of the pathogens. Anaplasma phagocytophilum was most common (40.0%, 2/5) in Sonoma chipmunks (T. sonomae) from Marin county and B. burgdorferi most common (37.5%, 27/72) in redwood chipmunks (T. ochrogenys) from Mendocino county. RF Borrelia spp. was detected in 2% (6/297) of redwood chipmunks in Mendocino county and 10% (1/10) of both least (T. minimus) and lodgepole (T. speciosus) chipmunks in the western Sierra. Exposure to SFG Rickettsia spp. was found in the Northern Coastal region (Del Norte, Humboldt and Mendocino counties) and in the northern and western Sierra in several species of chipmunks. Y. pestis infection was found only in the western Sierra-in a yellow-pine (T. amoenus) and a long-eared (T. quadrimaculatus) chipmunk. Though more data are needed to thoroughly understand the roles that different chipmunk species play in disease transmission, our findings suggest that some chipmunk species may be more important to the maintenance of vector-borne diseases than others within each geographic area.

  6. Contrasting stress responses of two co-occurring chipmunk species (Tamias alpinus and T. speciosus).

    PubMed

    Hammond, Talisin T; Palme, Rupert; Lacey, Eileen A

    2015-01-15

    Glucocorticoid (GC) hormones are important mediators of responses to environmental conditions. Accordingly, differences in GC physiology may contribute to interspecific variation in response to anthropogenically-induced patterns of climate change. To begin exploring this possibility, we validated the use of fecal cortisol/corticosterone metabolites (FCM) to measure baseline glucocorticoid levels in two species of co-occurring chipmunks that have exhibited markedly different patterns of response to environmental change. In Yosemite National Park, the alpine chipmunk (Tamias alpinus) has undergone a significant upward contraction of its elevational range over the past century; in contrast, the lodgepole chipmunk (Tamiasspeciosus) has experienced no significant change in elevational distribution over this period. To determine if GC levels in these species vary in response to external stimuli and to assess whether these responses differ between species, we compared FCM levels for the same individuals (1) at the time of capture in the field, (2) after a short period of captivity, and (3) after adrenocorticotropic hormone (ACTH), (4) handling, and (5) trapping challenges conducted while these animals were held in captivity. Our analyses indicate that T. alpinus was more responsive to several of these changes in external conditions. Although both species displayed a significant FCM response to ACTH challenge, only T. alpinus showed a significant response to our handling challenge and to captive housing conditions. These findings underscore the importance of species-specific validation studies and support the potential for studies of GC physiology to generate insights into interspecific differences in response to environmental change. Copyright © 2014 Elsevier Inc. All rights reserved.

  7. Ecology, distribution, and predictive occurrence modeling of Palmers chipmunk (Tamias palmeri): a high-elevation small mammal endemic to the Spring Mountains in southern Nevada, USA

    USGS Publications Warehouse

    Lowrey, Chris E.; Longshore, Kathleen M.; Riddle, Brett R.; Mantooth, Stacy

    2016-01-01

    Although montane sky islands surrounded by desert scrub and shrub steppe comprise a large part of the biological diversity of the Basin and Range Province of southwestern North America, comprehensive ecological and population demographic studies for high-elevation small mammals within these areas are rare. Here, we examine the ecology and population parameters of the Palmer’s chipmunk (Tamias palmeri) in the Spring Mountains of southern Nevada, and present a predictive GIS-based distribution and probability of occurrence model at both home range and geographic spatial scales. Logistic regression analyses and Akaike Information Criterion model selection found variables of forest type, slope, and distance to water sources as predictive of chipmunk occurrence at the geographic scale. At the home range scale, increasing population density, decreasing overstory canopy cover, and decreasing understory canopy cover contributed to increased survival rates.

  8. Infectivity to experimental rodents of Cryptosporidium parvum oocysts from Siberian chipmunks (Tamias sibiricus) originated in the People's Republic of China.

    PubMed

    Matsui, T; Fujino, T; Kajima, J; Tsuji, M

    2000-05-01

    We isolated Cryptosporidium parvum-type oocysts from naturally infected siberian chipmunks which originated in the People's Republic of China and examined the infectivity to rodents as experimental animals. The naturally infected chipmunks did not show any clinical symptoms. The oocysts were 4.8 x 4.2 microm on average in size. They were ovoid and morphologically similar to the C. parvum oocysts isolated from human and cattle. Experimental rodents were inoculated with 1.6 x 10(6) original oocysts each. SCID mice began to shed oocysts on day 7 and the OPG value was 10(5) from 50 days. The oocysts were found from ICR mice on days 13 and 16 by only sugar flotation method, however, any oocysts were not detected from the rats, guinea pigs and rabbits until 30 days. Two infected SCID mice were necropsied on days 100 and 102 and examined for coccidian organisms. Merozoites and oocysts were found in the low part of jejunum and ileum, however, no parasites were detected in the stomach. Consequently, it was considered that the present species was C. parvum and was probably genotype 2 from result of infectivity to rodents.

  9. Mapping human risk of infection with Borrelia burgdorferi sensu lato, the agent of Lyme borreliosis, in a periurban forest in France.

    PubMed

    Vourc'h, G; Abrial, D; Bord, S; Jacquot, M; Masséglia, S; Poux, V; Pisanu, B; Bailly, X; Chapuis, J-L

    2016-07-01

    Lyme borreliosis is a major zoonosis in Europe, with estimates of over 26,000 cases per year in France alone. The etiological agents are spirochete bacteria that belong to the Borrelia burgdorferi sensu lato (s. l.) complex and are transmitted by hard ticks among a large range of vertebrate hosts. In Europe, the tick Ixodes ricinus is the main vector. In the absence of a vaccine and given the current difficulties to diagnose and treat chronic Lyme syndromes, there is urgent need for prevention. In this context, accurate information on the spatial patterns of risk of exposure to ticks is of prime importance for public health. The objective of our study was to provide a snapshot map of the risk of human infection with B. burgdorferi s. l. pathogens in a periurban forest at a high resolution, and to analyze the factors that contribute to variation in this risk. Field monitoring took place over three weeks in May 2011 in the suburban Sénart forest (3,200ha; southeast of Paris), which receives over 3 million people annually. We sampled ticks over the entire forest area (from 220 forest stands with a total area of 35,200m(2)) and quantified the density of questing nymphs (DON), the prevalence of infection among nymphs (NIP), and the density of infected nymphs (DIN), which is the most important predictor of the human risk of Lyme borreliosis. For each of these response variables, we explored the relative roles of weather (saturation deficit), hosts (abundance indices of ungulates and Tamias sibiricus, an introduced rodent species), vegetation and forest cover, superficial soil composition, and the distance to forest roads. In total, 19,546 questing nymphs were collected and the presence of B. burgdorferi s. l. was tested in 3,903 nymphs by qPCR. The mean DON was 5.6 nymphs per 10m(2) (standard deviation=10.4) with an average NIP of 10.1% (standard deviation=0.11). The highest DIN was 8.9 infected nymphs per 10m(2), with a mean of 0.59 (standard deviation=0.6). Our

  10. Susceptibility of northern corn rootworm Diabrotica barberi (Coleoptera: Chrysomelidae) to mCry3A and eCry3.1Ab Bacillus thuringiensis proteins.

    PubMed

    Oyediran, Isaac O; Matthews, Phillip; Palekar, Narendra; French, Wade; Conville, Jared; Burd, Tony

    2016-12-01

    The susceptibility of the northern corn rootworm Diabrotica barberi (Smith & Lawrence) to mCry3A and eCry3.1Ab proteins derived from Bacillus thuringiensis (Bt) was determined using a diet bioassay. Northern corn rootworm neonates were exposed to different concentrations of mCry3A and eCry3.1Ab, incorporated into artificial diet. Larval mortality was evaluated after 7 d. The mCry3A and eCry3.1Ab proteins were found to be toxic to the northern corn rootworm larvae. The LC 50 and LC 99 values for mCry3A were 5.13 and 2482.31 μg/mL, respectively. For eCry3.1Ab, the LC 50 and LC 99 values were 0.49 and 213.01 μg/mL. Based on the estimated lethal concentrations, eCry3.1Ab protein was more efficacious to northern corn rootworm larvae than mCry3A. These lethal concentration values will be used as diagnostic doses for routine annual monitoring for change in susceptibility of field collected northern corn rootworm to mCry3A, and eCry3.1Ab toxins. © 2015 Institute of Zoology, Chinese Academy of Sciences.

  11. FLOTAC for diagnosis of endo-parasites in pet squirrels in southern Italy.

    PubMed

    d'Ovidio, D; Rinaldi, L; Ianniello, D; Donnelly, T M; Pepe, P; Capasso, M; Cringoli, G

    2014-02-24

    The present study investigated the occurrence of endoparasites in pet squirrels in southern Italy. Fresh fecal samples were collected from 50 asymptomatic pet squirrels belonging to five different species (Callosciurus finlaysonii, n=6, C. prevosti, n=6; Tamias striatus, n=26, T. sibiricus, n=10; Sciurus carolinensis, n=2) housed both in pet shops and/or in private residences. All fecal samples were processed using the FLOTAC pellet technique to identify and count helminth eggs/larvae and protozoan cysts/oocysts. In addition, to detect Cryptosporidium spp. and Giardia spp. the samples were analyzed by the Remel Xpect(®) immunoassay. Helminth eggs were detected in 9 out of 50 squirrels. Specifically, eggs of Dicrocoelium dendriticum were found in 5 squirrels (C. finlaysonii, n=2; C. prevosti, n=2; T. striatus, n=1); eggs of the pinworm Syphacia spp. in 3 squirrels (C. prevosti, n=2; T. striatus, n=1); and eggs of gastrointestinal nematoda (Nippostrongylus-like) were found in 1 subject (C. prevosti). Finally, two squirrels (C. prevosti) had multiple parasitic infections with D. dendriticum and Capillaria hepatica, and with D. dendriticum and Strongyloides spp., respectively. None of the samples were positive for Cryptosporidium spp. or Giardia spp. or any other protozoa (e.g. Eimeria). To the authors' knowledge, this is the first report of a D. dendriticum natural infection in pet rodents. Copyright © 2013 Elsevier B.V. All rights reserved.

  12. Molecular dynamics study of the structural and dynamic characteristics of the polyextremophilic short-chain dehydrogenase from the Thermococcus sibiricus archaeon and its homologues

    NASA Astrophysics Data System (ADS)

    Popinako, Anna V.; Antonov, Mikhail Yu.; Bezsudnova, Ekaterina Yu.; Prokopiev, Georgiy A.; Popov, Vladimir O.

    2017-11-01

    The study of structural adaptations of proteins from polyextremophilic organisms using computational molecular dynamics method is appealing because the obtained knowledge can be applied to construction of synthetic proteins with high activity and stability in polyextreme media which is useful for many industrial applications. To investigate molecular adaptations to high temperature, we have focused on a superthermostable short-chain dehydrogenase TsAdh319 from the Thermococcus sibiricus polyextremophilic archaeon and its closest structural homologues. Molecular dynamics method is widely used for molecular structure refinement, investigation of biological macromolecules motion, and, consequently, for interpreting the results of certain biophysical experiments. We performed molecular dynamics simulations of the proteins at different temperatures. Comparison of root mean square fluctuations (RMSF) of the atoms in thermophilic alcohol dehydrogenases (ADHs) at 300 K and 358 K revealed the existence of stable residues at 358 K. These residues surround the active site and form a "nucleus of rigidity" in thermophilic ADHs. The results of our studies suggest that the existence of the "nucleus of rigidity" is crucial for the stability of TsAdh319. Absence of the "nucleus of rigidity" in non-thermally stable proteins causes fluctuations throughout the protein, especially on the surface, triggering the process of denaturation at high temperatures.

  13. Reconsidering the effects of tannin on seed dispersal by rodents: evidence from enclosure and field experiments with artificial seeds.

    PubMed

    Zhang, Mingming; Steele, Michael A; Yi, Xianfeng

    2013-11-01

    The question of how tannin affects feeding and hoarding preferences of rodents still remains poorly understood, in part, because it is difficult to control for other seed traits when considering the sole effect of tannin. Here, we constructed a series of artificial 'seeds' with different tannin levels, made from wheat flour, peanut powder and hydrolysable tannins, to determine the direct effects of tannin on both feeding and hoarding preferences. We first presented 'seeds' to individual rodents of two species (Tamias sibiricus and Apodemus peninsulae) confined in semi-natural enclosures and then monitored patterns of seed dispersal and consumption by free-ranging animals in a temperate forest in the Xiaoxing'an Mountains, Heilongjiang Province of China. Our results showed that small rodents displayed a significant preference for low-tannin 'seeds' for both consumption and caching in both captive and field experiments. Moreover, our two-year study consistently showed that tannin concentration was significantly and negatively correlated with the number of cached 'seeds' at both the individual and population levels. Seed size, compared with tannin concentrations, appeared to have little effect on dispersal distances and the number of 'seeds' cached. Low-tannin 'seeds' tended to be dispersed greater distances by rodents in the field than those with higher levels of tannin. These results failed to support those of previous reports indicating that acorns containing higher tannins are more likely to be cached by food hoarding animals. Copyright © 2013 Elsevier B.V. All rights reserved.

  14. Camptosorus sibiricus rupr aqueous extract prevents lung tumorigenesis via dual effects against ROS and DNA damage.

    PubMed

    He, Shugui; Ou, Rilan; Wang, Wensheng; Ji, Liyan; Gao, Hui; Zhu, Yuanfeng; Liu, Xiaomin; Zheng, Hongming; Liu, Zhongqiu; Wu, Peng; Lu, Linlin

    2018-06-28

    Camptosorus sibiricus Rupr (CSR) is a widely used herbal medicine with antivasculitis, antitrauma, and antitumor effects. However, the effect of CSR aqueous extract on B[a]P-initiated tumorigenesis and the underlying mechanism remain unclear. Moreover, the compounds in CSR aqueous extract need to be identified and structurally characterized. We aim to investigate the chemopreventive effect of CSR and the underlying molecular mechanism. A B[a]P-stimulated normal cell model (BEAS.2B) and lung adenocarcinoma animal model were established on A/J mice. In B[a]P-treated BEAS.2B cells, the protective effects of CSR aqueous extract on B[a]P-induced DNA damage and ROS production were evaluated through flow cytometry, Western blot, real-time quantitative PCR, single-cell gel electrophoresis, and immunofluorescence. Moreover, a model of B[a]P-initiated lung adenocarcinoma was established on A/J mice to determine the chemopreventive effect of CSR in vivo. The underlying mechanism was analyzed via immunohistochemistry and microscopy. Furthermore, the new compounds in CSR aqueous extract were isolated and structurally characterized using IR, HR-ESI-MS, and 1D and 2D NMR spectroscopy. CSR effectively suppressed ROS production by re-activating Nrf2-mediated reductases HO-1 and NQO-1. Simultaneously, CSR attenuated the DNA damage of BEAS.2B cells in the presence of B[a]P. Moreover, CSR at 1.5 and 3 g/kg significantly suppressed tumorigenesis with tumor inhibition ratios of 36.65% and 65.80%, respectively. The tumor volume, tumor size, and multiplicity of B[a]P-induced lung adenocarcinoma were effectively decreased by CSR in vivo. After extracting and identifying the compounds in CSR aqueous extract, three new triterpene saponins were isolated and characterized structurally. CSR aqueous extract prevents lung tumorigenesis by exerting dual effects against ROS and DNA damage, suggesting that CSR is a novel and effective agent for B[a]P-induced carcinogenesis. Moreover, by isolating

  15. Detection of Bartonella tamiae, Coxiella burnetii and rickettsiae in arthropods and tissues from wild and domestic animals in northeastern Algeria.

    PubMed

    Leulmi, Hamza; Aouadi, Atef; Bitam, Idir; Bessas, Amina; Benakhla, Ahmed; Raoult, Didier; Parola, Philippe

    2016-01-20

    In recent years, the scope and importance of emergent vector-borne diseases has increased dramatically. In Algeria, only limited information is currently available concerning the presence and prevalence of these zoonotic diseases. For this reason, we conducted a survey of hematophagous ectoparasites of domestic mammals and/or spleens of wild animals in El Tarf and Souk Ahras, Algeria. Using real-time PCR, standard PCR and sequencing, the presence of Bartonella spp., Rickettsia spp., Borrelia spp. and Coxiella burnetii was evaluated in 268/1626 ticks, 136 fleas, 11 Nycteribiidae flies and 16 spleens of domestic and/or wild animals from the El Tarf and Souk Ahras areas. For the first time in Algeria, Bartonella tamiae was detected in 12/19 (63.2%) Ixodes vespertilionis ticks, 8/11 (72.7%) Nycteribiidae spp. flies and in 6/10 (60%) bat spleens (Chiroptera spp.). DNA from Coxiella burnetii, the agent of Q fever, was also identified in 3/19 (15.8%) I. vespertilionis from bats. Rickettsia slovaca, the agent of tick-borne lymphadenopathy, was detected in 1/1 (100%) Haemaphysalis punctata and 2/3 (66.7%) Dermacentor marginatus ticks collected from two boars (Sus scrofa algira) respectively. Ri. massiliae, an agent of spotted fever, was detected in 38/94 (40.4%) Rhipicephalus sanguineus sensu lato collected from cattle, sheep, dogs, boars and jackals. DNA of Ri. aeschlimannii was detected in 6/20 (30%) Hyalomma anatolicum excavatum and 6/20 (30%) Hy. scupense from cattle. Finally, Ri. felis, an emerging rickettsial pathogen, was detected in 80/110 (72.7%) Archaeopsylla erinacei and 2/2 (100%) Ctenocephalides felis of hedgehogs (Atelerix algirus). In this study, we expanded knowledge about the repertoire of ticks and flea-borne bacteria present in ectoparasites and/or tissues of domestic and wild animals in Algeria.

  16. Fine structure of mouthparts and feeding performance of Pyrrhocoris sibiricus Kuschakevich with remarks on the specialization of sensilla and stylets for seed feeding

    PubMed Central

    Wang, Yan; Dai, Wu

    2017-01-01

    Mouthpart structure and feeding behavior in the temperate firebug, Pyrrhocoris sibiricus Kuschakevich, an important pest that feeds on seeds of leguminous and gramineous plants, are described for the first time. Mouthparts were observed using scanning electron microscopy to examine the external morphology, distribution and abundance of sensilla on mouthparts. Feeding performance by adults on both seeds and shoots were observed using a binocular microscope. The four-segmented labium contains 3 types of sensilla trichodea, 3 types of sensilla basiconica, 1 type of sensilla placodea and 1 type of sensilla campaniformia. Among them, sensilla trichodea are most abundant. The tripartite apex of the labium consists of two lateral lobes and an apical plate. Each lateral lobe possesses a field of 12 thick-walled uniporous peg sensilla and long non-porous hair sensilla. The mandibular stylet tips have three central teeth and two pairs of lateral teeth, which may help in penetrating hard seed coats. A series of scale-like projections are present on the inner surface of the mandibular stylets. The externally smooth maxillary stylets interlock to form a larger food canal and a smaller salivary canal, and there are five tubercles near the tip of the right stylet. Cross-sections of the stylet fascicle show that each mandibular stylet has a dendritic canal. The adult feeding process involves several steps, including exploring and puncturing of the host epidermis, a probing phase, an engorgement phase, and removal of the mouthparts from the host tissue. The structure and function of the mouthparts are adapted for the seed feeding habits. PMID:28481941

  17. Genetic diversity among eight Dendrolimus species in Eurasia (Lepidoptera: Lasiocampidae) inferred from mitochondrial COI and COII, and nuclear ITS2 markers.

    PubMed

    Kononov, Alexander; Ustyantsev, Kirill; Wang, Baode; Mastro, Victor C; Fet, Victor; Blinov, Alexander; Baranchikov, Yuri

    2016-12-22

    Moths of genus Dendrolimus (Lepidoptera: Lasiocampidae) are among the major pests of coniferous forests worldwide. Taxonomy and nomenclature of this genus are not entirely established, and there are many species with a controversial taxonomic position. We present a comparative evolutionary analysis of the most economically important Dendrolimus species in Eurasia. Our analysis was based on the nucleotide sequences of COI and COII mitochondrial genes and ITS2 spacer of nuclear ribosomal genes. All known sequences were extracted from GenBank. Additional 112 new sequences were identified for 28 specimens of D. sibiricus, D. pini, and D. superans from five regions of Siberia and the Russian Far East to be able to compare the disparate data from all previous studies. In total, 528 sequences were used in phylogenetic analysis. Two clusters of closely related species in Dendrolimus were found. The first cluster includes D. pini, D. sibiricus, and D. superans; and the second, D. spectabilis, D. punctatus, and D. tabulaeformis. Species D. houi and D. kikuchii appear to be the most basal in the genus. Genetic difference among the second cluster species is very low in contrast to the first cluster species. Phylogenetic position D. tabulaeformis as a subspecies was supported. It was found that D. sibiricus recently separated from D. superans. Integration of D. sibiricus mitochondrial DNA sequences and the spread of this species to the west of Eurasia have been established as the cause of the unjustified allocation of a new species: D. kilmez. Our study further clarifies taxonomic problems in the genus and gives more complete information on the genetic structure of D. pini, D. sibiricus, and D. superans.

  18. Habitat interaction between two species of chipmunk in the Basin and Range Province of Nevada

    USGS Publications Warehouse

    Lowrey, Christopher; Longshore, Kathleen M.

    2013-01-01

    Interspecies interactions can affect how species are distributed, put constraints on habitat expansion, and reduce the fundamental niche of the affected species. Using logistic regression, we analyzed and compared 174 Tamias palmeri and 94 Tamias panamintinus within an isolated mountain range of the Basin and Range Province of southern Nevada. Tamias panamintinus was more likely to use pinyon/ponderosa/fir mixed forests than pinyon alone, compared to random sites. In the presence of T palmeri, however, interaction analyses indicated T. panamintinus was less likely to occupy the mixed forests and more likely near large rocks on southern aspects. This specie s-by-habitat interaction data suggest that T. palmeri excludes T panamintinus from areas of potentially suitable habitat. Climate change may adversely affect species of restricted distribution. Habitat isolation and species interactions in this region may thus increase survival risks as climate temperatures rise.

  19. Hantaan virus surveillance targeting small mammals at nightmare range, a high elevation military training area, Gyeonggi Province, Republic of Korea.

    PubMed

    Klein, Terry A; Kim, Heung-Chul; Chong, Sung-Tae; Kim, Jeong-Ah; Lee, Sook-Young; Kim, Won-Keun; Nunn, Peter V; Song, Jin-Won

    2015-01-01

    Rodent-borne disease surveillance was conducted at Nightmare Range (NM-R), near the demilitarized zone in northeast Gyeonggi Province, Republic of Korea, to identify hemorrhagic fever with renal syndrome (HFRS) risks for a mountainous high-elevation (500 m) military training site. Monthly surveys were conducted from January 2008-December 2009. A total of 1,720 small mammals were captured belonging to the Orders Rodentia [Families, Sciuridae (1 species) and Muridae (7 species)] and Soricomorpha [Family, Soricidae (1species)]. Apodemus agrarius, the primary reservoir for Hantaan virus (HTNV), accounted for 89.9% (1,546) of all small mammals captured, followed by Myodes regulus (4.0%), Crocidura lasiura (3.9%), Micromys minutus (1.4%), Mus musculus (0.3%), Microtus fortis (0.2%), Apodemus peninsulae (0.2%), Tamias sibiricus (0.1%), and Rattus norvegicus (<0.1%). Three species were antibody-positive (Ab+) for hantaviruses: A. agrarius (8.2%), M. minutus (4.2%), and C. lasiura (1.5%). HTNV specific RNA was detected in 93/127 Ab+ A. agrarius, while Imjin virus specific RNA was detected in 1/1 Ab+ C. lasiura. Overall, hantavirus Ab+ rates for A. agrarius increased with weight (age) and were significantly higher among males (10.9%) than females (5.1%) (P<0.0001). High A. agrarius gravid rates during the fall (August-September) were associated with peak numbers of HFRS cases in Korea that followed high gravid rates. From 79 RT-PCR positive A. agrarius, 12 HTNV RNA samples were sequenced and compared phylogenetically based on a 320 nt sequence from the GC glycoprotein-encoding M segment. These results demonstrate that the HTNV isolates from NM-R are distinctly separated from HTNV isolated from the People's Republic of China. These studies provide for improved disease risk assessments that identify military activities, rodent HTNV rates, and other factors associated with the transmission of hantaviruses during field training exercises.

  20. [A comparative study on seed germination of 15 grass species in Keeqin Sandyland].

    PubMed

    Liu, Zhimin; Li, Xuehua; Li, Rongping; Jiang, Deming; Cao, Chengyou

    2003-09-01

    A laboratory study was made on the germination characteristics of freshly-collected seeds of grass species at the Wulanaodu area of Keeqin Sandyland in Eastern Inner-Mongolia. Of the 15 species examined, 8 species including Clinelymus dahuricus, Cleistogenes squarrosa, Pappophorum boreale, Spodiopogon sibiricus, Phragmites communis, Chloris virgata, Arundinella hirta, Pennisetum alopecuroides had a germination rate of over 80%, but 4 species including Echinochloa hispidula, Hemarthria compressa, Tragus berteronianus and Setaria viridis had a value of less than 10%. Spodiopogon sibiricus, Eragrostis pilosa, Phragmites communis, Chloris virgata, Clinelymus dahuricus, Pappophorum boreale, Digitaria cilliaris and Cleistogenes squrrosa began to germinate within 1-3 days after the test began, while Setaria viridis, Tragus berteronianus and Hemarthria compressa failed to germinate in a period of more than 10 days. For the species such as Digitaria cilliaris, Echinochloa hispidula, Phragmites communis, Eragrostis pilosa and Spodiopogon sibiricus, their germination period was less than 10 days, while Clinelymus dahuricus and Pappophorum boreale had a germination period of more than 20 days. The days required for half the final germination rate to be reached were: 2 days for Chloris virgata, 3 days for Phragmites communis, 4 days for Spodiopogon sibiricus, 5 days for Clinelymus dahuricus and Cleistogenes squarrosa, 7 days for Arundinella hirta and Pappophorum boreale, and 10 days for Pennisetum alopecuriodes. Compared with the Sheffield region in Britain, the Wulanaodu area of Kerqin Sandyland had a higher proportion of annul grasses with a low germination rate and a longer germination period, and the perennial grasses at the Wulanaodu area had an approximately same germination rate, but a longer germination period. During germination, ruderals showed the potential for risk-sharring, and thus, they had a relatively higher disturbance-resistance capacity.

  1. Revision of the genus Ptomaphagus Hellwig from eastern Asia (Coleoptera, Leiodidae, Cholevinae).

    PubMed

    Wang, Cheng-Bin; Perreau, Michel; Růžička, Jan; Nishikawa, Masaaki

    2017-01-01

    The species belonging to the genus Ptomaphagus Hellwig, 1795 (Coleoptera, Leiodidae, Cholevinae, Ptomaphagini) from eastern Asia are assigned to three species groups. Group yasutoshii has a single species: P. (s. str.) yasutoshii Nishikawa, 1993 from Taiwan, China. Group nepalensis with three species: P. (s. str.) nepalensis Perreau, 1988 from Nepal and P. (s. str.) masumotoi Nishikawa, 2011 from Thailand are redescribed, and P. (s. str.) piccoloi Wang, Růžička, Nishikawa, Perreau & Hayashi, 2016 is recorded for the first time from China (Zhejiang). Group sibiricus with seven species, including two newly described Chinese ones P. (s. str.) funiu sp. n. from Henan, and P. (s. str.) haba sp. n. from Yunnan, and five known species: P. (s. str.) chenggongi Wang, Nishikawa, Perreau, Růžička & Hayashi, 2016, P. (s. str.) hayashii Wang, Růžička, Perreau, Nishikawa & Park, 2016, P. (s. str.) kuntzeni Sokolowski, 1957 (distribution records from Myanmar excluded), P. (s. str.) sibiricus Jeannel, 1934 and P. (s. str.) tingtingtae Wang, Nishikawa, Perreau, Růžička & Hayashi, 2016. Specimens of other undescribed species of the group sibiricus are also recorded, revealing a high diversity of this genus in eastern Asia, especially in central and north Sichuan, China, which essentially remains to be investigated. Relevant morphological characters of the examined species are illustrated with colour plates, and their known distributions are mapped. A key to species of Ptomaphagus from eastern Asia is provided.

  2. Response of small mammals to aerial applications of the nucleopolyhedrosis virus of the gypsy moth, Lymantria dispar

    Treesearch

    R.A. Lautenschlager; H. Rothenbacher; J.D. Podgwaite

    1978-01-01

    Resident populations of white-footed mice, Peromyscus leucopus Rafinesque, red-backed voles, Clethrionomys gapperi Vigers, opossums, Didelphis marsupialis L., chipmunks, Tamias striatus L., and raccoons, Procyon lotor L., were evaluated to detect any short term effects from...

  3. Small mammal mycophagy response to variations in green-tree retention

    Treesearch

    Katherine M. Jacobs; Daniel L. Luoma

    2007-01-01

    We studied the effects of six green-tree retention levels and patterns on the diets of northern flying squirrels (Glaucomys sabrinus), Townsend's chipmunks (Tamias townsendii), Siskiyou chipmunks (T. siskiyou), western red-backed voles (Myodes calijamicus), and southern red-backed voles (...

  4. A new species of Anthocoris (Hemiptera: Heteroptera: Anthocoridae) from western North America

    USDA-ARS?s Scientific Manuscript database

    Anthocoris aquilivenis Lewis, n. sp. is described from the mountainous areas of western North America. It is compared to the Nearctic species Anthocoris tomentosus Péricart and Anthocoris antevolens White, and to Anthocoris sibiricus Reuter, a related Palearctic species. Illustrations are provided o...

  5. Experimental manipulation of spatial heterogeneity in Douglas-fir forests: effects on squirrels.

    Treesearch

    A.B. Carey

    2001-01-01

    Squirrel communities simultaneously composed of abundant populations of Glaucomys, Tamias, and Tamiasciurus are: (1) a result of high production of seeds and fruiting bodies by forest plants and fungi and complexity of ecosystem structure, composition, and function; (2) indicative of high carrying capacity...

  6. POLYMORPHIC MICROSATELLITE LOCI FROM NORTHERN AND MEXICAN CORN ROOTWORMS (INSECTA: COLEOPTERA: CHRYSOMELIDAE) AND CROSS-AMPLIFICATION WITH OTHER DIABROTICA SPP

    EPA Science Inventory

    The northern corn rootworm (Diabrotica barberi) and Mexican corn rootworm (Diabrotica virgifera zeae) are significant agricultural pests. For the northern corn rootworm, and to a lesser extent, the Mexican corn rootworm, high resolution molecular markers are needed. Here we pres...

  7. The effects of natural and induced short-term floods on four sugarcane accessions

    USDA-ARS?s Scientific Manuscript database

    A study was initiated at the USDA/ARS Subtropical Horticulture Research Station, Miami, FL to evaluate the potential for flood tolerance in several accessions from Saccharum barberi (Jeswiet) and S. sinense (Roxb. Amend. Jeswiet) sugarcane genera. The experimental design included 4 accessions, Kewal...

  8. Effects of wildfire severity on small mammals in northern Arizona ponderosa pine forests

    Treesearch

    Sean C. Kyle; William M. Block

    2000-01-01

    We examined effects of a varied-severity wildfire on the community structure of small mammals and populations of the 2 most abundant species, the deer mouse (Peromyscus maniculatus) and the gray-collared chipmunk (Tamias cinereicollis), in northern Arizona ponderosa pine (Pinus ponderosa) forests. We examined 2...

  9. The Russians are coming - aren't they? Siberian moth in European forests

    Treesearch

    Yuri N. Baranchikov; Vladimir M. Pet' ko; Vladimir L. Ponomarev

    2007-01-01

    Dendrolimus superans sibiricus Tschtv. (Lepidoptera: Lasiocampidae) is the most destructive defoliator of the coniferous taiga forest in Siberia and the Russian Far East. Outbreaks of this pest species are the primary biological factor influencing change in forest cover in the southern taiga subzone with enormous ecological and social consequences (...

  10. Screening for corn rootworm (Coleoptera: Chrysomelidae) resistance to transgenic Bt corn in North Dakota

    USDA-ARS?s Scientific Manuscript database

    Western (WCR), Diabrotica virgifera virgifera LeConte, and northern corn rootworms (NCR), D. barberi Smith & Lawrence, are major economic pests of corn in much of the U.S. Corn Belt. Western corn rootworm resistance to transgenic corn expressing Bt (Bacillus thuringiensis) endotoxins has been confi...

  11. Effect of transgenic corn hybrids and a soil insecticide on corn rootworm (Coleoptera: Chrysomelidae) beetle emergence in North Dakota

    USDA-ARS?s Scientific Manuscript database

    Northern, Diabrotica barberi Smith & Lawrence, and western corn rootworms, D. virgifera virgifera LeConte, are economic pests of corn, Zea mays L. (Poaceae) in North Dakota. Many area corn growers rely on transgenic Bt (Bacillus thuringiensis) corn hybrids to manage corn rootworms. Our objective was...

  12. Registration of ‘HoCP 04-838’ sugarcane

    USDA-ARS?s Scientific Manuscript database

    ‘HoCP 04-838’ sugarcane (an interspecific hybrid of S. officinarum, S. barberi Jeswiet, S. spontaneum, and S. sinense Roxb. amend. Jeswiet) was selected and evaluated by scientists at the USDA-ARS, working cooperatively with the Louisiana State University Agricultural Center, and the American Sugar ...

  13. Ecological scale and forest development: squirrels, dietary fungi, and vascular plants in managed and unmanaged forests.

    Treesearch

    A.B. Carey; J. Kershner; B. Biswell; L.S. Dominguez de Toledo

    1999-01-01

    Understanding ecological processes and their spatial scales is key to managing ecosystems for biodiversity, especially for species associated with late-seral forest. We focused on 2 species of squirrel (Sciuridae: northern flying squirrel, Glaucomys sabrinus, and Townsend's chipmunk, Tamias townsendii) in a crosssectional survey of managed and natural stands in...

  14. Environmental Compliance Assessment and Management System Program (ECAMP). U.S. Air Force European Economic Community (EEC)

    DTIC Science & Technology

    1993-09-01

    alboranensis Anthemis glabemrma Artemisia granatensis Artemisia laciniata Aster pyrenacus Aster sibiricus Centaurea heldreichii Centaurea horrida Centaurea...kalambakensi s Centaurea lactiflora Centaurea Iinaresii Centaurea megarensis Centaurea niederi Centaurea peucedanifolia Centaurea princeps Crepis...50 Table 448 (continued) COMIPOSrrAE Anthemis glaberrima (Rech. f.) Greuter Artemisia granatensis Boiss. Aster pyrenacus Desf. ex DC. Aster sorrentinil

  15. An experimental burn to restore a moth-killed boreal conifer forest, Krasnoyarsk Region, Russia

    Treesearch

    E.N. Valendik; J.C. Brissette; Ye. K. Kisilyakhov; R.J. Lasko; S.V. Verkhovets; S.T. Eubanks; I.V. Kosov; A. Yu. Lantukh

    2006-01-01

    Mechanical treatment and prescribed fire were used to restore a mixed conifer stand (Picea-Abies-Pinus) following mortality from an outbreak of Siberian moth (Dendrolimus superans sibiricus). Moth-killed stands often become dominated by Calamagrostis, a sod-forming grass. The large amount of woody debris and the sod hinder coniferous seedling establishment and...

  16. Registration of ‘Ho 00-961’ sugarcane

    USDA-ARS?s Scientific Manuscript database

    ‘Ho 00-961’ (Reg. No., PI) sugarcane (a complex hybrid of Saccharum officinarum L., S. spontaneum L., S. barberi Jeswiet, and S. sinense Roxb. amend. Jeswiet) was selected by the USDA-ARS Sugarcane Research Unit, and evaluated cooperatively with the Louisiana State University Agricultural Center, an...

  17. Response of eastern chipmunks to single application spring prescribed fires on the Fernow Experimental Forest

    Treesearch

    E.L. Rowan; W.M. Ford; S.B. Castleberry; J.L. Rodrigue; T.M. Schuler; T.M. Schuler

    2005-01-01

    We used radiotelemetry to examine the effects of spring prescribed fire for preharvest oak (Quercus spp.) shelterwood management on eastern chipmunk (Tamias striatus) home-range attributes and burrow use on the Fernow Experimental Forest in the central Appalachian Mountains of West Virginia. Results for 21 chipmunks showed that prescribed fire had little discernable...

  18. Something All His Own: The NBA's Grant Hill Hopes His Collection of African American Art Will Inspire, Excite a Younger Generation

    ERIC Educational Resources Information Center

    Keels, Crystal L.

    2004-01-01

    NBA star Grant Hill is known for his skills on the basketball court, his marriage to Grammy award-winning singer, Tamia, and, most recently, for his courageous comeback after several surgeries that jeopardized his professional basketball career. Yet, Hill's off-the-court activities currently are being considered as exemplary as his athletic…

  19. Landscape disturbance and sporadic hybridization complicate field identification of chipmunks

    Treesearch

    Christina F. Frare; Marjorie D. Matocq; Chris R. Feldman; Angela M. White; Patricia N. Manley; Kathleen D. Jermstad; Evon R. Hekkala

    2016-01-01

    Chipmunks (Tamias spp.) in western North America are important for their numerical abundance, their role in pathogen transmission, and the composition and structure of food webs. As such, land management agencies (e.g., U.S. Forest Service) often conduct field surveys to monitor the diversity and abundance of chipmunk species as a measure...

  20. Insights on the host associations and geographic distribution of Hymenolepis folkertsi (Cestoda: Hymenolepididae) among rodents across temperate latitudes of North America.

    PubMed

    Hoberg, E P; Makarikov, A A; Tkach, V V; Meagher, S; Nims, T N; Eckerlin, R P; Galbreath, K E

    2016-12-01

    Synoptic data and an understanding of helminth parasite diversity among diverse rodent assemblages across temperate latitudes of North America remain remarkably incomplete. Renewed attention to comprehensive survey and inventory to establish the structure of biodiverse faunas is essential in providing indicators and proxies for identifying the outcomes of accelerating change linked to climate warming and anthropogenic forcing. Subsequent to the description of Hymenolepis folkertsi in the oldfield mouse, Peromyscus polionotus, additional specimens of hymenolepidids were collected or discovered in archived museum repositories from multiple species of deer mice (Peromyscus maniculatus, Peromyscus leucopus), the golden mouse (Ochrotomys nuttalli), chipmunks (Tamias striatus, Tamias amoenus), the 13-lined ground squirrel (Ictidomys tridecemlineatus), and tree squirrels (Sciurus carolinensis, Sciurus niger) from disjunct localities in the USA spanning southern Georgia, Virginia, Pennsylvania, Connecticut, the Upper Peninsula of Michigan, Wisconsin, and central Idaho. Specimens were largely consistent morphologically with the original description of H. folkertsi. Initial DNA sequence data, from a portion of the mitochondrial NADH dehydrogenase subunit 1, demonstrated intraspecific variation among three apparently geographically isolated populations attributed to H. folkertsi (uncorrected genetic distances of 2.7 % (Idaho and Michigan), 2.4 % (Virginia + Pennsylvania and Michigan), and 1.89 % (VA + PA and ID). Geography rather than host association explains the distribution and occurrence of H. folkertsi, and host colonization among deer mice, chipmunks, and other sciurids within regional sites is indicated. Genetic divergence revealed across localities for H. folkertsi suggests historically isolated populations, consistent with extended evolutionary and biogeographic trajectories among hymenolepidids and species of Peromyscus and Tamias in North America. Field

  1. A Grounded Theory Approach to Understanding Ethical Leadership with School Leaders in Southern Nigeria: A Perspective of Three Primary Schools

    ERIC Educational Resources Information Center

    Egbufor, Dorothy Chukwudumebi

    2017-01-01

    The chief aim of this study was to develop a grounded theory of ethical leadership with school leaders in Southern Nigeria, utilizing a qualitative constructivist paradigm and multiple case study design. There is growing interest in public service of ethics (Barberis, 2001). The study of ethics has been a part of the [school] leadership erudition…

  2. [New species of the Rhinonyssid mites (Gamasina: Rhinonyssidae) from birds of Russia and neighboring countries].

    PubMed

    Butenko, O M; Staniukovich, M K

    2001-01-01

    Four new species of the nasal mite family Rhinonyssidae collected in different regions of the former USSR are described: Neonyssus (Otocorinyssus) alaudae sp. n. from Alauda arvensis L. (Alaudidae, Passeriformes) from Turkmenistan; Rhinonyssus clangulae sp. n. from Clangula hyemalis (L.) (Anatidae, Anseriformes) from Yakutia; R. marilae sp. n. from Aythya marilae L. (Anatidae, Anseriformes) from the Russian Far East; Locustellonyssus sibiricus sp. n. from Locustella certhiola (Pall.) (Sylviidae, Passeriformes) from Siberia.

  3. [Transmission risk of Trypanosoma cruzi in Metztitlán municipality from Hidalgo state, México, by characterization of domiciliary units and their entomologic indexes].

    PubMed

    Becerril, Marco A; Angeles-Pérez, Vidal; Noguez-García, Julio Cr; Imbert-Palafox, José L

    2010-01-01

    In order to determine the risk of transmission of Trypanosoma cruzi by triatomines in Metztitlan municipality, Hidalgo State, Mexico, entomological indexes were calculated and the characteristics of dwellings were described. A transversal, retrospective, descriptive, and observational study was performed by means of an intentional not probabilistic and expertise sampling from January to December of 2005 in 10 localities in which presence of triatomines were investigated either intra or peridomestic environmental in 699 houses. Building material and presence of infected triatomines with T. cruzi were registered to determine entomologic indexes. The triatomine species collected were: Triatoma barberi (Usinger) and T. mexicana (Herrich-Schaeffer) (Hemiptera: Reduviidae). The results indicated that natural infection index varied from 7.7% to 50%; colonization index reached 80%; infestation index varied from 7.7% to 25%; dispersion index was 70%. Stone-walled houses were more infested. We can conclude that it is necessary to establish T. cruzi transmission control measures against triatomines in localities from Metztitlan, primarily in stone-walled houses where T. barberi occurs, as it was the most important vector species in the transmission of T. cruzi in this municipality.

  4. Mosquitoes in Moose Country: A Mosquito Survey of Northern Minnesota.

    PubMed

    Kinsley, A C; Moon, R D; Johnson, K; Carstensen, M; Neitzel, D; Craft, M E

    2016-06-01

    An adult mosquito survey was conducted at 12 sites using carbon dioxide traps in northern Minnesota throughout the summer of 2012. Specimens were counted, identified to species, sorted into pools, and tested for eastern equine encephalitis (EEEV) and West Nile virus (WNV). Our findings extend the known range of Culiseta melanura, Anopheles barberi, and An. quadrimaculatus and document the presence and abundance of 27 other mosquito taxa in the region. None of the pools tested positive for EEEV or WNV.

  5. The lestevoides species group of the genus Geodromicus Redtenbacher, 1857 (Insecta: Coleoptera: Staphylinidae: Omaliinae).

    PubMed

    Shavrin, Alexey V

    2018-02-07

    The lestevoides species group of the genus Geodromicus Redtenbacher, 1857 is erected. Seventeen species of the group from the East Asia, including G. smetanai sp. n. (Sichuan, China), are reviewed and keyed. Geodromicus iburinus (Watanabe, 1990) and G. nipponensis (Watanabe, 1990) are revalidated; G. hermani (Watanabe, 1991) syn.n. is synonymized with G. nipponensis (Watanabe, 1990); G. kirschenblati Tichomirova, 1973 is resynonymized with G. sibiricus Bernhauer, 1915; lectotypes for G. chinensis Bernhauer, 1938 and G. lestevoides (Sharp, 1889) are designated. The distributional maps for eight species are provided; the genital structures of fourteen species are figured.

  6. Male lifetime mating success in relation to body size in Diabrotica barberi

    USDA-ARS?s Scientific Manuscript database

    Body size is often an important component of male lifetime mating success in insects, especially when males are capable of mating several times over their lifespan. We paired either a large or small male northern corn rootworm with a female of random size and noted copulation success. We observed co...

  7. Section Editors

    NASA Astrophysics Data System (ADS)

    Groep, D. L.; Bonacorsi, D.

    2014-06-01

    1. Data Acquisition, Trigger and Controls Niko NeufeldCERNniko.neufeld@cern.ch Tassos BeliasDemokritosbelias@inp.demokritos.gr Andrew NormanFNALanorman@fnal.gov Vivian O'DellFNALodell@fnal.gov 2. Event Processing, Simulation and Analysis Rolf SeusterTRIUMFseuster@cern.ch Florian UhligGSIf.uhlig@gsi.de Lorenzo MonetaCERNLorenzo.Moneta@cern.ch Pete ElmerPrincetonpeter.elmer@cern.ch 3. Distributed Processing and Data Handling Nurcan OzturkU Texas Arlingtonnurcan@uta.edu Stefan RoiserCERNstefan.roiser@cern.ch Robert IllingworthFNAL Davide SalomoniINFN CNAFDavide.Salomoni@cnaf.infn.it Jeff TemplonNikheftemplon@nikhef.nl 4. Data Stores, Data Bases, and Storage Systems David LangeLLNLlange6@llnl.gov Wahid BhimjiU Edinburghwbhimji@staffmail.ed.ac.uk Dario BarberisGenovaDario.Barberis@cern.ch Patrick FuhrmannDESYpatrick.fuhrmann@desy.de Igor MandrichenkoFNALivm@fnal.gov Mark van de SandenSURF SARA sanden@sara.nl 5. Software Engineering, Parallelism & Multi-Core Solveig AlbrandLPSC/IN2P3solveig.albrand@lpsc.in2p3.fr Francesco GiacominiINFN CNAFfrancesco.giacomini@cnaf.infn.it Liz SextonFNALsexton@fnal.gov Benedikt HegnerCERNbenedikt.hegner@cern.ch Simon PattonLBNLSJPatton@lbl.gov Jim KowalkowskiFNAL jbk@fnal.gov 6. Facilities, Infrastructures, Networking and Collaborative Tools Maria GironeCERNMaria.Girone@cern.ch Ian CollierSTFC RALian.collier@stfc.ac.uk Burt HolzmanFNALburt@fnal.gov Brian Bockelman U Nebraskabbockelm@cse.unl.edu Alessandro de SalvoRoma 1Alessandro.DeSalvo@ROMA1.INFN.IT Helge MeinhardCERN Helge.Meinhard@cern.ch Ray PasetesFNAL rayp@fnal.gov Steven GoldfarbU Michigan Steven.Goldfarb@cern.ch

  8. Natural infection of Cryptosporidium muris (Apicomplexa: Cryptosporiidae) in Siberian chipmunks.

    PubMed

    Hůrková, Lada; Hajdusek, Ondrej; Modrý, David

    2003-04-01

    Coprologic examination of nine Siberian chipmunks (Eutamias sibiricus) imported from Southeast Asia revealed infection with Cryptosporidium sp. Experimental inoculation of BALB/c mice proved their susceptibility to the infection. Infected mice shed oocysts 14-35 days postinfection. Oocyst morphology was similar to that reported for C. muris in previous studies, oocysts were 8.1 (7.0-9.0) x 5.9 (5.0-6.5) microns. Clinical signs were absent in naturally infected chipmunks and experimental mice. Histologic examinations of mice revealed numerous developmental stages of C. muris in the glandular stomach. Analysis of partial small subunit rRNA gene sequences confirmed identity of these isolates as C. muris. Our results represent the first report of C. muris in members of the family Sciuridae.

  9. A contribution to mayfly studies of Western Mongolia (Insecta, Ephemeroptera)

    PubMed Central

    Erdenee, Bolortsetseg; Maasri, Alain; Gelhaus, Jon K.; Bayartogtokh, Badamdorj

    2016-01-01

    Abstract Streams in the Mongolian Altai Mountains are mostly fed from glaciers and are extreme conditions for mayflies because of high elevation, low temperatures and low annual precipitation. Previous information about mayflies of Western Mongolia is scarce, but with this study a total of 38 species belonging to 26 genera and subgenera and 8 families of mayflies has been recorded in the Mongolian Altai region. Study material was entirely imagos and collected from 78 sites during expeditions led by the Mongolian Aquatic Insect Survey in 2008, 2009 and 2010. Raptobaetopus tenellus, Caenis luctuosa and Caenis rivulorum are recorded as new to the fauna of Mongolia, and there are new distribution records for Ameletus montanus, Baetis (Acentrella) lapponica, Baetis sibiricus, Baetis (Labiobaetis) attrebatinus, Centroptilum luteolum, Procloeon pennulatum, Ephemerella aurivillii, Serratella setigera, Ephemera sachalinensis, Ecdyonurus (Afronurus) abracadabrus, Cinygmula kurenzovi, Ecdyonurus (Afghanurus) vicinus and Epeorus (Belovius) pellucidus from the Mongolian Altai region. Baetis vernus and Ephemerella aurivillii are the most frequently encountered species in this region. PMID:28174499

  10. Mid-Wisconsinan vertebrates and their environment from January Cave, Alberta, Canada

    NASA Astrophysics Data System (ADS)

    Burns, James A.

    1991-01-01

    January Cave, in the Rocky Mountains of southwestern Alberta, has yielded vertebrate remains from a coprocenosis of mid-Wisconsinan-age. Taphonomic analysis indicates accumulation by raptors, mostly owls, and mammalian carnivores. The vertebrate record, together with pollen analysis, indicates that cool, dry conditions prevailed in an extensive tundra-like environment, with prairie elements in the valleys below. Thirty-four mammalian taxa have been recovered from January Cave. Today, some of these species (e.g., Lemmus sibiricus and Dicrostonyx torquatus) do not coexist with others (e.g., Cynomys sp., Mustela nigripes, Vulpes velox, and Lagurus curtatus). Therefore, the January Cave local fauna represents a "nonanalog" mammalian community characteristic of the late Pleistocene. It suggests that the region enjoyed an equable climate, with reduced climatic extremes but still cool, further supporting a mid-Wisconsinan age estimate for the fauna. It is the first major, small vertebrate fauna of its age to be reported from Alberta.

  11. Serologic evidence for Borrelia hermsii infection in rodents on federally owned recreational areas in California.

    PubMed

    Fritz, Curtis L; Payne, Jessica R; Schwan, Tom G

    2013-06-01

    Tick-borne relapsing fever (TBRF) is endemic in mountainous regions of the western United States. In California, the principal agent is the spirochete Borrelia hermsii, which is transmitted by the argasid tick Ornithodoros hermsi. Humans are at risk of TBRF when infected ticks leave an abandoned rodent nest in quest of a blood meal. Rodents are the primary vertebrate hosts for B. hermsii. Sciurid rodents were collected from 23 sites in California between August, 2006, and September, 2008, and tested for serum antibodies to B. hermsii by immunoblot using a whole-cell sonicate and a specific antigen, glycerophosphodiester phosphodiesterase (GlpQ). Antibodies were detected in 20% of rodents; seroprevalence was highest (36%) in chipmunks (Tamias spp). Seroprevalence in chipmunks was highest in the Sierra Nevada (41%) and Mono (43%) ecoregions and between 1900 and 2300 meters elevation (43%). The serological studies described here are effective in implicating the primary vertebrate hosts involved in the maintenance of the ticks and spirochetes in regions endemic for TBRF.

  12. Morphological and morphometric analysis of scutella of six species and two subspecies of Triatoma (Hemiptera: Reduviidae) in Mexico.

    PubMed

    Rivas, Nancy; Sánchez-Cordero, Víctor; Camacho, Alejandro D; Córdoba-Aguilar, Alejandro; Alejandre-Aguilar, Ricardo

    2017-12-01

    Morphological characters can be used to distinguish the vast majority of triatomine species, but the existence of high levels of phenotypic plasticity and recently diverged species can lead to erroneous determinations. To approach this problem, we analyzed the male and female morphologies of the scutella of Triatoma barberi, T. dimidiata, T. lecticularia, T. mexicana, T. recurva, T. rubida, and two sub-species, T. protracta protracta and T. protracta nahuatlae. Scutellum samples were observed by scanning electron microscopy and subjected to morphological analysis and morphometric investigation using a canonical discriminant analysis. The results revealed differences primarily in central depression shape, posterior process, and vestiture. We observed clear dimension-based differences in scutellum morphometry in all the taxa under study, providing sound evidence for species and subspecies differentiation. On the other hand, there is no difference between sexes in T. lecticularia, T. protracta protracta, and T. protracta nahuatlae. Our methodology can be implemented to differentiate species of the genus Triatoma. © 2017 The Society for Vector Ecology.

  13. Ecologic Niche Modeling and Potential Reservoirs for Chagas Disease, Mexico.

    PubMed Central

    Sánchez-Cordero, Victor; Ben Beard, C.; Ramsey, Janine M.

    2002-01-01

    Ecologic niche modeling may improve our understanding of epidemiologically relevant vector and parasite-reservoir distributions. We used this tool to identify host relationships of Triatoma species implicated in transmission of Chagas disease. Associations have been documented between the protracta complex (Triatoma: Triatominae: Reduviidae) with packrat species (Neotoma spp.), providing an excellent case study for the broader challenge of developing hypotheses of association. Species pairs that were identified coincided exactly with those in previous studies, suggesting that local interactions between Triatoma and Neotoma species and subspecies have implications at a geographic level. Nothing is known about sylvatic associates of T. barberi, which are considered the primary Chagas vector in Mexico; its geographic distribution coincided closely with that of N. mexicana, suggesting interaction. The presence of the species was confirmed in two regions where it had been predicted but not previously collected. This approach may help in identifying Chagas disease risk areas, planning vector-control strategies, and exploring parasite-reservoir associations for other emerging diseases. PMID:12095431

  14. First web-based database on total phenolics and oxygen radical absorbance capacity (ORAC) of fruits produced and consumed within the south Andes region of South America.

    PubMed

    Speisky, Hernan; López-Alarcón, Camilo; Gómez, Maritza; Fuentes, Jocelyn; Sandoval-Acuña, Cristian

    2012-09-12

    This paper reports the first database on antioxidants contained in fruits produced and consumed within the south Andes region of South America. The database ( www.portalantioxidantes.com ) contains over 500 total phenolics (TP) and ORAC values for more than 120 species/varieties of fruits. All analyses were conducted by a single ISO/IEC 17025-certified laboratory. The characterization comprised native berries such as maqui ( Aristotelia chilensis ), murtilla ( Ugni molinae ), and calafate ( Barberis microphylla ), which largely outscored all other studied fruits. Major differences in TP and ORAC were observed as a function of the fruit variety in berries, avocado, cherries, and apples. In fruits such as pears, apples, apricots, and peaches, a significant part of the TP and ORAC was accounted for by the antioxidants present in the peel. These data should be useful to estimate the fruit-based intake of TP and, through the ORAC data, their antioxidant-related contribution to the diet of south Andes populations.

  15. Directional selection effects on patterns of phenotypic (co)variation in wild populations

    PubMed Central

    Patton, J. L.; Hubbe, A.; Marroig, G.

    2016-01-01

    Phenotypic (co)variation is a prerequisite for evolutionary change, and understanding how (co)variation evolves is of crucial importance to the biological sciences. Theoretical models predict that under directional selection, phenotypic (co)variation should evolve in step with the underlying adaptive landscape, increasing the degree of correlation among co-selected traits as well as the amount of genetic variance in the direction of selection. Whether either of these outcomes occurs in natural populations is an open question and thus an important gap in evolutionary theory. Here, we documented changes in the phenotypic (co)variation structure in two separate natural populations in each of two chipmunk species (Tamias alpinus and T. speciosus) undergoing directional selection. In populations where selection was strongest (those of T. alpinus), we observed changes, at least for one population, in phenotypic (co)variation that matched theoretical expectations, namely an increase of both phenotypic integration and (co)variance in the direction of selection and a re-alignment of the major axis of variation with the selection gradient. PMID:27881744

  16. Directional selection effects on patterns of phenotypic (co)variation in wild populations.

    PubMed

    Assis, A P A; Patton, J L; Hubbe, A; Marroig, G

    2016-11-30

    Phenotypic (co)variation is a prerequisite for evolutionary change, and understanding how (co)variation evolves is of crucial importance to the biological sciences. Theoretical models predict that under directional selection, phenotypic (co)variation should evolve in step with the underlying adaptive landscape, increasing the degree of correlation among co-selected traits as well as the amount of genetic variance in the direction of selection. Whether either of these outcomes occurs in natural populations is an open question and thus an important gap in evolutionary theory. Here, we documented changes in the phenotypic (co)variation structure in two separate natural populations in each of two chipmunk species (Tamias alpinus and T. speciosus) undergoing directional selection. In populations where selection was strongest (those of T. alpinus), we observed changes, at least for one population, in phenotypic (co)variation that matched theoretical expectations, namely an increase of both phenotypic integration and (co)variance in the direction of selection and a re-alignment of the major axis of variation with the selection gradient. © 2016 The Author(s).

  17. Prevalence of Human-Active and Variant 1 Strains of the Tick-Borne Pathogen Anaplasma phagocytophilum in Hosts and Forests of Eastern North America

    PubMed Central

    Keesing, Felicia; McHenry, Diana J.; Hersh, Michelle; Tibbetts, Michael; Brunner, Jesse L.; Killilea, Mary; LoGiudice, Kathleen; Schmidt, Kenneth A.; Ostfeld, Richard S.

    2014-01-01

    Anaplasmosis is an emerging infectious disease caused by infection with the bacterium Anaplasma phagocytophilum. In the eastern United States, A. phagocytophilum is transmitted to hosts through the bite of the blacklegged tick, Ixodes scapularis. We determined the realized reservoir competence of 14 species of common vertebrate hosts for ticks by establishing the probability that each species transmits two important strains of A. phagocytophilum (A. phagocytophilum human-active, which causes human cases, and A. phagocytophilum variant 1, which does not) to feeding larval ticks. We also sampled questing nymphal ticks from ∼150 sites in a single county over 2 years and sampled over 6 years at one location. White-footed mice (Peromyscus leucopus) and Eastern chipmunks (Tamias striatus) were the most competent reservoirs for infection with the A. phagocytophilum human-active strain. Across the county, prevalence in ticks for both strains together was 8.3%; ticks were more than two times as likely to be infected with A. phagocytophilum human-active as A. phagocytophilum variant 1. PMID:24865688

  18. The Th1/Th2/Th17/Treg paradigm induced by stachydrine hydrochloride reduces uterine bleeding in RU486-induced abortion mice.

    PubMed

    Li, Xia; Wang, Bin; Li, Yuzhu; Wang, Li; Zhao, Xiangzhong; Zhou, Xianbin; Guo, Yuqi; Jiang, Guosheng; Yao, Chengfang

    2013-01-09

    The Th1/Th2/Th17/Treg paradigm plays an important role in achieving maternal-fetal immunotolerance and participates in RU486-induced abortion. Excessive uterine bleeding is the most common side effect of RU486-induced abortion; however, its etiopathogenesis has not been fully understood. Therefore, elucidating the correlation between the Th1/Th2/Th17/Treg paradigm and the volume of uterine bleeding may offer novel therapeutic target for reducing uterine bleeding in RU486-induced abortion. Leonurus sibiricus has been used in clinics to reduce postpartum hemorrhage with low toxicity and high efficiency; however, the effective constituents and therapeutic mechanism have not been described. Stachydrine hydrochloride is the main constituent of L. sibiricus, therefore L. sibiricus is regarded as a candidate for reducing uterine bleeding in RU486-induced abortion mice by regulating the Th1/Th2/Th17/Treg paradigm. The purpose of this study was to determine the Th1/Th2/Th17/Treg paradigm in uterine bleeding of RU486-induced abortion mice and to elucidate the immunopharmacologic effects of stachydrine hydrochloride on inducing the Th1/Th2/Th17/Treg paradigm in reducing the uterine bleeding volume in RU486-induced abortion mice. To investigate the Th1/Th2/Th17/Treg paradigm in uterine bleeding during RU486-induced abortion mice, pregnant BALB/c mice were treated with high- and low-dose RU486 (1.5mg/kg and 0.9 mg/kg, respectively), and the serum progesterone (P(4)) protein level, uterine bleeding volume, and proportions of Th1/Th2/Th17/Treg cells in mice at the maternal-fetal interface were detected by ELISA assay, alkaline hematin photometric assay, and flow cytometry, respectively. To determine the regulatory effect of stachydrine hydrochloride on the Th1/Th2/Th17/Treg paradigm in vitro, splenocytes of non-pregnant mice were separated and treated with P(4,) RU486, and/or stachydrine hydrochloride (10(-5)M, 10(-4)M, and 10(-3)M, respectively). The proportions of Th1/Th2/Th17

  19. Multi-Sensor Mapping of Diffuse Degassing of C-O-H Compounds in Terrestrial Hydrothermal Systems

    NASA Astrophysics Data System (ADS)

    Schwandner, F. M.; Shock, E. L.

    2004-12-01

    In-situ single-sensor detection and mapping of diffuse degassing phenomena in hydrothermal and volcanic areas can be used to elucidate subsurface tectonic structures, assess emission rates, and to monitor emission variability (Williams 1985; Chiodini et al. 1996, Werner et al., 2003). More than one technique has been deployed to measure several gas species simultaneously (e.g., Crenshaw et al. 1982), and correlations of one gas species (usually CO2) with physical parameters like heat flux (Brombach et al., 2001), or with one other gas species (Rn, He) have been demonstrated (Barberi & Carapezza 1994; Williams-Jones et al., 2000). Recently, correlations of multiple gas species with one another were reported (Schwandner et al., 2004), leading to the possibility of quantitative mapping of subsurface hydrothermal chemical processes by simultaneous measurement of reaction partners and products that continuously and diffusely degas. In the present study, we joined a fully-quantitative multi-sensor instrument (Draeger Multiwarn II) to a modified accumulation-chamber sensing method (Chiodini et al., 1996) and measured diffuse degassing of CH4, H2, CO2, CO, and H2S. In this approach, each batch of gas that is recirculated through the detector is simultaneously analyzed by all sensors. To test this approach we chose two magmatically influenced, hydrothermally active areas at Yellowstone National Park (USA): Sylvan Springs and the Greater Obsidian Pool Area. The area near Obsidian Pool was previously studied during a diffuse CO2 degassing campaign (Werner & Brantley, 2004). Preliminary results show that elevated reduced gas emissions appear to be most prominent near hydrothermal pools, whereas CO2-dominated degassing anomalies highlight subsurface tectonic structures. This multimodal distribution allows us to distinguish deep degassing sources (CO2 anomalies) from shallow localized hydrothermal processes (reduced gas anomalies). The results permit us to positively identify

  20. Northern Goshawk diet in Minnesota: An Analysis using video recording systems

    USGS Publications Warehouse

    Smithers, B.L.; Boal, C.W.; Andersen, D.E.

    2005-01-01

    We used video-recording systems to collect diet information at 13 Northern Goshawk (Accipiter gentilis) nests in Minnesota during the 2000, 2001, and 2002 breeding seasons. We collected 4871 hr of video footage, from which 652 prey deliveries were recorded. The majority of prey deliveries identified were mammals (62%), whereas birds (38%) composed a smaller proportion of diet. Mammals accounted for 61% of biomass delivered, and avian prey items accounted for 39% of prey biomass. Sciurids and leporids accounted for 70% of the identified prey. Red squirrel (Tamiasciurus hudsonicus), eastern chipmunk (Tamias striatus), and snowshoe hare (Lepus americanus) were the dominant mammals identified in the diet, while American Crow (Corvus brachyrhynchos) and Ruffed Grouse (Bonasa umbellus) were the dominant avian prey delivered to nests. On average, breeding goshawks delivered 2.12 prey items/d, and each delivery averaged 275 g for a total of 551 g delivered/d. However, daily (P < 0.001) and hourly (P = 0.01) delivery rates varied among nests. Delivery rates (P = 0.01) and biomass delivered (P = 0.038) increased with brood size. Diversity and equitability of prey used was similar among nests and was low throughout the study area, most likely due to the dominance of red squirrel in the diet. ?? 2005 The Raptor Research Foundation, Inc.

  1. Atlas of Mexican Triatominae (Reduviidae: Hemiptera) and vector transmission of Chagas disease

    PubMed Central

    Ramsey, Janine M; Peterson, A Townsend; Carmona-Castro, Oscar; Moo-Llanes, David A; Nakazawa, Yoshinori; Butrick, Morgan; Tun-Ku, Ezequiel; de la Cruz-Félix, Keynes; Ibarra-Cerdeña, Carlos N

    2015-01-01

    Chagas disease is one of the most important yet neglected parasitic diseases in Mexico and is transmitted by Triatominae. Nineteen of the 31 Mexican triatomine species have been consistently found to invade human houses and all have been found to be naturally infected with Trypanosoma cruzi. The present paper aims to produce a state-of-knowledge atlas of Mexican triatomines and analyse their geographic associations with T. cruzi, human demographics and landscape modification. Ecological niche models (ENMs) were constructed for the 19 species with more than 10 records in North America, as well as for T. cruzi. The 2010 Mexican national census and the 2007 National Forestry Inventory were used to analyse overlap patterns with ENMs. Niche breadth was greatest in species from the semiarid Nearctic Region, whereas species richness was associated with topographic heterogeneity in the Neotropical Region, particularly along the Pacific Coast. Three species, Triatoma longipennis, Triatoma mexicana and Triatoma barberi, overlapped with the greatest numbers of human communities, but these communities had the lowest rural/urban population ratios. Triatomine vectors have urbanised in most regions, demonstrating a high tolerance to human-modified habitats and broadened historical ranges, exposing more than 88% of the Mexican population and leaving few areas in Mexico without the potential for T. cruzi transmission. PMID:25993505

  2. The effect of predation on begging-call evolution in nestling wood warblers.

    PubMed

    Haskell

    1999-04-01

    I combined a comparative study of begging in ground- and tree-nesting wood warblers (Parulidae) with experimental measures of the predation costs of warbler begging calls. Throughout their development, ground-nesting warbler nestlings had significantly higher-frequency begging calls than did tree-nesting warblers. There was also a trend for ground-nesting birds to have less rapidly modulated calls. There were no consistent associations between nesting site and the amplitude of the calls. Using miniature walkie-talkies hidden inside artificial nests, I reciprocally transplanted the begging calls of 5- and 8-day-old black-throated blue warblers, Dendroica caerulescens (tree-nesting) and ovenbirds, Seiurus aurocapillus (ground-nesting) and measured the corresponding changes in rates of nest predation. For the begging calls of 8-day-old nestlings, but not those of 5-day-olds, the calls of the tree-nesting species coming from ground nests incurred greater costs than did the calls of ground nesters. The reciprocal transplant had little effect on the rate of predation. Tooth imprints on clay eggs placed in artificial nests indicated that eastern chipmunks, Tamias striatus, were responsible for the increased cost of begging for black-throated blue calls coming from the ground. These data suggest that nest predation may be responsible for maintaining some of the interspecific differences in the acoustic structure of begging calls. Copyright 1999 The Association for the Study of Animal Behaviour.

  3. Climate, Deer, Rodents, and Acorns as Determinants of Variation in Lyme-Disease Risk

    PubMed Central

    Canham, Charles D; Oggenfuss, Kelly; Winchcombe, Raymond J; Keesing, Felicia

    2006-01-01

    Risk of human exposure to vector-borne zoonotic pathogens is a function of the abundance and infection prevalence of vectors. We assessed the determinants of Lyme-disease risk (density and Borrelia burgdorferi-infection prevalence of nymphal Ixodes scapularis ticks) over 13 y on several field plots within eastern deciduous forests in the epicenter of US Lyme disease (Dutchess County, New York). We used a model comparison approach to simultaneously test the importance of ambient growing-season temperature, precipitation, two indices of deer (Odocoileus virginianus) abundance, and densities of white-footed mice (Peromyscus leucopus), eastern chipmunks (Tamias striatus), and acorns ( Quercus spp.), in both simple and multiple regression models, in predicting entomological risk. Indices of deer abundance had no predictive power, and precipitation in the current year and temperature in the prior year had only weak effects on entomological risk. The strongest predictors of a current year's risk were the prior year's abundance of mice and chipmunks and abundance of acorns 2 y previously. In no case did inclusion of deer or climate variables improve the predictive power of models based on rodents, acorns, or both. We conclude that interannual variation in entomological risk of exposure to Lyme disease is correlated positively with prior abundance of key hosts for the immature stages of the tick vector and with critical food resources for those hosts. PMID:16669698

  4. Infectious diseases in Mexico. A survey from 1995-2000.

    PubMed

    Flisser, Ana; Velasco-Villa, Andrés; Martínez-Campos, Carmen; González-Domínguez, Fernando; Briseño-García, Baltasar; García-Suárez, Rosario; Caballero-Servín, Angel; Hernández-Monroy, Irma; García-Lozano, Herlinda; Gutiérrez-Cogco, Lucina; Rodríguez-Angeles, Guadalupe; López-Martínez, Irma; Galindo-Virgen, Sonia; Vázquez-Campuzano, Roberto; Balandrano-Campos, Susana; Guzmán-Bracho, Carmen; Olivo-Díaz, Angélica; de la Rosa, Jorge; Magos, Clementina; Escobar-Gutiérrez, Alejandro; Correa, Dolores

    2002-01-01

    Data obtained at a central laboratory for emerging, re-emerging, and other infectious diseases in Mexico from 1995-2000 are presented. An outstanding increase of DEN-3 circulation was identified. Aedes aegypti, the dengue vector, is widely distributed. Leptospirosis has become the most important differential diagnosis for dengue. Identification of rabies virus variants allowed cataloging of new transmitters of rabies. Rotavirus showed a clear seasonal distribution, while different proportions of pathogenic classes of Escherichia coli under endemic and outbreak conditions were seen. Serotypes of several bacteria are reported as well as the sources of isolation and frequency of Shigella, Salmonella, and Vibrio cholerae. Rise and disappearance of cholera could be followed along the past decade. Influenza strains were identified, as were several pathogens causing sexually transmitted infections. Laboratory support was important for surveillance after Hurricane Mitch. Multidrug-resistant strains of Mycobacterium tuberculosis are emerging and primary resistance is very high. It is now mandatory to search for antibodies to Trypanosoma cruzi in blood banks. Triatoma barberi, a peridomestic bug, is the main vector of Chagas disease. Localized cutaneous leishmaniosis increased in regions having a guerrilla element in Chiapas. Modern immunodiagnostic techniques are used for control studies of cysticercosis and similar techniques were recently standardized for Trichinella spiralis detection. Low iodine values in children's urine were found in several Mexican states; therefore, use of iodized salt should be encouraged.

  5. Molecular systematics of the Middle American genus Hypopachus (Anura: Microhylidae)

    PubMed Central

    Greenbaum, Eli; Smith, Eric N.; de Sá, Rafael O.

    2011-01-01

    We present the first phylogenetic study on the widespread Middle American microhylid frog genus Hypopachus. Partial sequences of mitochondrial (12S and 16S ribosomal RNA) and nuclear (rhodopsin) genes (1275 bp total) were analyzed from 43 samples of Hypopachus, three currently recognized species of Gastrophryne, and seven arthroleptid, brevicipitid and microhylid outgroup taxa. Maximum parsimony (PAUP), maximum likelihood (RAxML) and Bayesian inference (MrBayes) optimality criteria were used for phylogenetic analyses, and BEAST was used to estimate divergence dates of major clades. Population-level analyses were conducted with the programs NETWORK and Arlequin. Results confirm the placement of Hypopachus and Gastrophryne as sister taxa, but the latter genus was strongly supported as paraphyletic. The African phrynomerine genus Phrynomantis was recovered as the sister taxon to a monophyletic Chiasmocleis, rendering our well-supported clade of gastrophrynines paraphyletic. Hypopachus barberi was supported as a disjunctly distributed highland species, and we recovered a basal split in lowland populations of Hypopachus variolosus from the Pacific versant of Mexico and elsewhere in the Mesoamerican lowlands. Dating analyses from BEAST estimate speciation within the genus Hypopachus occurred in the late Miocene/early Pliocene for most clades. Previous studies have not found bioacoustic or morphological differences among these lowland clades, and our molecular data support the continued recognition of two species in the genus Hypopachus. PMID:21798357

  6. Forced Folds and Craters of Elevation in the Afar

    NASA Astrophysics Data System (ADS)

    Hetherington, Rachel; Mussetti, Giulio; Hagos, Miruts; Deering, Chad; Corti, Giacomo; Magee, Craig; Ian, Bastow; van Wyk de Vries, Benjamin; Marques, Alvaro

    2015-04-01

    Uplifts caused by magma intrusion have been observed and mapped since the pioneering work of von Buch two hundred years ago. Von Buch's "Craters of elevation theory", developed in the Auvergne and Canaries, was, unfortunately, discredited and mostly forgotten until recent work has shown that the forced folds mapped in seismic sections in sedimentary basins are the same type of feature. Also, these magmatic bulges are being found on an increasing number of volcanoes. The Danakil region of Ethiopia contains a superb range of forced folds with craters of elevation, which we have mapped using Google Earth and other satellite imagery, and some ground truthing. This preliminary work provides a geological map that is inspired from the 1970 Barberi and Vallet map, and which sets out in detail the structure and lava flow units of this area. We describe the main structures of each uplift, and suggest a preliminary general structural evolutionary pattern that provides a model for future research. This work has been untertaken by a group from multiple universities sharing interpretations and data in an open format. We aim to continue this work to study these superb geological features. Their superb nature and preservation is such that we would also press for them to be made a geoheritage reserve of global importance: Either a Geopark or a World Heritage site.

  7. An Experimental Test of Competition among Mice, Chipmunks, and Squirrels in Deciduous Forest Fragments

    PubMed Central

    Brunner, Jesse L.; Duerr, Shannon; Keesing, Felicia; Killilea, Mary; Vuong, Holly; Ostfeld, Richard S.

    2013-01-01

    Mixed hardwood forests of the northeast United States support a guild of granivorous/omnivorous rodents including gray squirrels (Sciurus carolinensis), eastern chipmunks (Tamias striatus), and white-footed mice (Peromyscus leucopus). These species coincide geographically, co-occur locally, and consume similar food resources. Despite their idiosyncratic responses to landscape and patch variables, patch occupancy models suggest that competition may influence their respective distributions and abundances, and accordingly their influence on the rest of the forest community. Experimental studies, however, are wanting. We present the result of a large-scale experiment in which we removed white-footed mice or gray squirrels from small, isolated forest fragments in Dutchess County, New York, and added these mammals to other fragments in order to alter the abundance of these two species. We then used mark–recapture analyses to quantify the population-level and individual-level effects on resident mice, squirrels, and chipmunks. Overall, we found little evidence of competition. There were essentially no within-season numerical responses to changes in the abundance of putative competitors. Moreover, while individual-level responses (apparent survival and capture probability) did vary with competitor densities in some models, these effects were often better explained by site-specific parameters and were restricted to few of the 19 sites we studied. With only weak or nonexistent competition among these three common rodent species, we expect their patterns of habitat occupancy and population dynamics to be largely independent of one another. PMID:23824654

  8. Unlocking the vault: next generation museum population genomics

    PubMed Central

    Bi, Ke; Linderoth, Tyler; Vanderpool, Dan; Good, Jeffrey M; Nielsen, Rasmus; Moritz, Craig

    2014-01-01

    Natural history museum collections provide unique resources for understanding how species respond to environmental change, including the abrupt, anthropogenic climate change of the past century. Ideally, researchers would conduct genome-scale screening of museum specimens to explore the evolutionary consequences of environmental changes, but to date such analyses have been severely limited by the numerous challenges of working with the highly degraded DNA typical of historic samples. Here we circumvent these challenges by using custom, multiplexed, exon-capture to enrich and sequence ~11,000 exons (~4Mb) from early 20TH century museum skins. We used this approach to test for changes in genomic diversity accompanying a climate-related range retraction in the alpine chipmunks (Tamias alpinus) in the high Sierra Nevada area of California, USA. We developed robust bioinformatic pipelines that rigorously detect and filter-out base misincorporations in DNA derived from skins, most of which likely resulted from post-mortem damage. Furthermore, to accommodate genotyping uncertainties associated with low-medium coverage data, we applied a recently developed probabilistic method to call SNPs and estimate allele frequencies and the joint site frequency spectrum. Our results show increased genetic subdivision following range retraction, but no change in overall genetic diversity at either non-synonymous or synonymous sites. This case study showcases the advantages of integrating emerging genomic and statistical tools in museum collection-based population genomic applications. Such technical advances greatly enhance the value of museum collections, even where a pre-existing reference is lacking, and points to a broad range of potential applications in evolutionary and conservation biology. PMID:24118668

  9. Effects of climate and exurban development on nest predation and predator presence in the southern Appalachian Mountains (USA).

    PubMed

    Lumpkin, Heather A; Pearson, Scott M; Turner, Monica G

    2012-08-01

    In the eastern United States, land-use and climate change have likely contributed to declines in the abundance of Neotropical migrant birds that occupy forest interiors, but the mechanisms are not well understood. We conducted a nest-predation experiment in southern Appalachian Mountain forests (North Carolina, U.S.A.) during the 2009 and 2010 breeding seasons to determine the effects of exurban development and temperature on predator presence and the average number of days until eggs in an artificial nest were disturbed by predators. We baited artificial nests with quail (Excalfactoria chinensi) eggs and monitored them for 18 days. We used clay eggs, track plates, and motion-triggered cameras to detect and identify nest predators. The average number of days a nest was undisturbed decreased as mean temperature increased and, to a lesser extent, as the density of buildings increased. Nests on the ground were more often depredated than those in trees, likely due to increased predation by opossum (Didelphis virginiana) and other carnivores. Raccoons (Procyon lotor), opossums, corvids (Corvus brachyrhynchos and Cyanocitta cristata), chipmunks (Tamias striatus), black bears (Ursus americanus), and domestic cats (Felis catus) were the most commonly detected predators. Presence of these predators did not vary as a function of mean temperature. Domestic cats and corvids were detected more frequently in plots with high rather than low densities of buildings. Forest-interior specialists and Neotropical migrants often nest in cool, high-elevation areas with low housing density. These bird species, especially those that nest on the ground, may be most vulnerable to increased nest predation if temperature and exurban development increase at higher elevations as anticipated. ©2012 Society for Conservation Biology.

  10. Unlocking the vault: next-generation museum population genomics.

    PubMed

    Bi, Ke; Linderoth, Tyler; Vanderpool, Dan; Good, Jeffrey M; Nielsen, Rasmus; Moritz, Craig

    2013-12-01

    Natural history museum collections provide unique resources for understanding how species respond to environmental change, including the abrupt, anthropogenic climate change of the past century. Ideally, researchers would conduct genome-scale screening of museum specimens to explore the evolutionary consequences of environmental changes, but to date such analyses have been severely limited by the numerous challenges of working with the highly degraded DNA typical of historic samples. Here, we circumvent these challenges by using custom, multiplexed, exon capture to enrich and sequence ~11,000 exons (~4 Mb) from early 20th-century museum skins. We used this approach to test for changes in genomic diversity accompanying a climate-related range retraction in the alpine chipmunks (Tamias alpinus) in the high Sierra Nevada area of California, USA. We developed robust bioinformatic pipelines that rigorously detect and filter out base misincorporations in DNA derived from skins, most of which likely resulted from postmortem damage. Furthermore, to accommodate genotyping uncertainties associated with low-medium coverage data, we applied a recently developed probabilistic method to call single-nucleotide polymorphisms and estimate allele frequencies and the joint site frequency spectrum. Our results show increased genetic subdivision following range retraction, but no change in overall genetic diversity at either nonsynonymous or synonymous sites. This case study showcases the advantages of integrating emerging genomic and statistical tools in museum collection-based population genomic applications. Such technical advances greatly enhance the value of museum collections, even where a pre-existing reference is lacking and points to a broad range of potential applications in evolutionary and conservation biology. © 2013 John Wiley & Sons Ltd.

  11. Cryptosporidium parvum Infection Involving Novel Genotypes in Wildlife from Lower New York State

    PubMed Central

    Perz, Joseph F.; Le Blancq, Sylvie M.

    2001-01-01

    Cryptosporidium, an enteric parasite of humans and a wide range of other mammals, presents numerous challenges to the supply of safe drinking water. We performed a wildlife survey, focusing on white-tailed deer and small mammals, to assess whether they may serve as environmental sources of Cryptosporidium. A PCR-based approach that permitted genetic characterization via sequence analysis was applied to wildlife fecal samples (n = 111) collected from September 1996 to July 1998 from three areas in lower New York State. Southern analysis revealed 22 fecal samples containing Cryptosporidium small-subunit (SSU) ribosomal DNA; these included 10 of 91 white-tailed deer (Odocoileus virginianus) samples, 3 of 5 chipmunk (Tamias striatus) samples, 1 of 2 white-footed mouse (Peromyscus leucopus) samples, 1 of 2 striped skunk (Mephitis mephitis) samples, 1 of 5 racoon (Procyon lotor) samples, and 6 of 6 muskrat (Ondatra zibethicus) samples. All of the 15 SSU PCR products sequenced were characterized as Cryptosporidium parvum; two were identical to genotype 2 (bovine), whereas the remainder belonged to two novel SSU sequence groups, designated genotypes 3 and 4. Genotype 3 comprised four deer-derived sequences, whereas genotype 4 included nine sequences from deer, mouse, chipmunk, and muskrat samples. PCR analysis was performed on the SSU-positive fecal samples for three other Cryptosporidium loci (dihydrofolate reductase, polythreonine-rich protein, and beta-tubulin), and 8 of 10 cloned PCR products were consistent with C. parvum genotype 2. These data provide evidence that there is sylvatic transmission of C. parvum involving deer and other small mammals. This study affirmed the importance of wildlife as potential sources of Cryptosporidium in the catchments of public water supplies. PMID:11229905

  12. Cryptosporidium parvum infection involving novel genotypes in wildlife from lower New York State.

    PubMed

    Perz, J F; Le Blancq, S M

    2001-03-01

    Cryptosporidium, an enteric parasite of humans and a wide range of other mammals, presents numerous challenges to the supply of safe drinking water. We performed a wildlife survey, focusing on white-tailed deer and small mammals, to assess whether they may serve as environmental sources of Cryptosporidium. A PCR-based approach that permitted genetic characterization via sequence analysis was applied to wildlife fecal samples (n = 111) collected from September 1996 to July 1998 from three areas in lower New York State. Southern analysis revealed 22 fecal samples containing Cryptosporidium small-subunit (SSU) ribosomal DNA; these included 10 of 91 white-tailed deer (Odocoileus virginianus) samples, 3 of 5 chipmunk (Tamias striatus) samples, 1 of 2 white-footed mouse (Peromyscus leucopus) samples, 1 of 2 striped skunk (Mephitis mephitis) samples, 1 of 5 racoon (Procyon lotor) samples, and 6 of 6 muskrat (Ondatra zibethicus) samples. All of the 15 SSU PCR products sequenced were characterized as Cryptosporidium parvum; two were identical to genotype 2 (bovine), whereas the remainder belonged to two novel SSU sequence groups, designated genotypes 3 and 4. Genotype 3 comprised four deer-derived sequences, whereas genotype 4 included nine sequences from deer, mouse, chipmunk, and muskrat samples. PCR analysis was performed on the SSU-positive fecal samples for three other Cryptosporidium loci (dihydrofolate reductase, polythreonine-rich protein, and beta-tubulin), and 8 of 10 cloned PCR products were consistent with C. parvum genotype 2. These data provide evidence that there is sylvatic transmission of C. parvum involving deer and other small mammals. This study affirmed the importance of wildlife as potential sources of Cryptosporidium in the catchments of public water supplies.

  13. Morphological and dietary responses of chipmunks to a century of climate change.

    PubMed

    Walsh, Rachel E; Aprígio Assis, Ana Paula; Patton, James L; Marroig, Gabriel; Dawson, Todd E; Lacey, Eileen A

    2016-09-01

    Predicting how individual taxa will respond to climatic change is challenging, in part because the impacts of environmental conditions can vary markedly, even among closely related species. Studies of chipmunks (Tamias spp.) in Yosemite National Park provide an important opportunity to explore the reasons for this variation in response. While the alpine chipmunk (T. alpinus) has undergone a significant elevational range contraction over the past century, the congeneric and partially sympatric lodgepole chipmunk (T. speciosus) has not experienced an elevational range shift during this period. As a first step toward identifying the factors underlying this difference in response, we examined evidence for dietary changes and changes in cranial morphology in these species over the past century. Stable isotope analyses of fur samples from modern and historical museum specimens of these species collected at the same localities indicated that signatures of dietary change were more pronounced in T. alpinus, although diet breadth did not differ consistently between the study species. Morphometric analyses of crania from these specimens revealed significant changes in cranial shape for T. alpinus, with less pronounced changes in shape for T. speciosus; evidence of selection on skull morphology was detected for T. alpinus, but not for T. speciosus. These results are consistent with growing evidence that T. alpinus is generally more responsive to environmental change than T. speciosus, but emphasize the complex and often geographically variable nature of such responses. Accordingly, future studies that make use of the taxonomically and spatially integrative approach employed here may prove particularly informative regarding relationships between environmental conditions, range changes, and patterns of phenotypic variation. © 2016 John Wiley & Sons Ltd.

  14. Sequence diversity among badnavirus isolates infecting black pepper and related species in India.

    PubMed

    Bhat, A I; Sasi, Shina; Revathy, K A; Deeshma, K P; Saji, K V

    2014-01-01

    The badnavirus, piper yellow mottle virus (PYMoV) is known to infect black pepper (Piper nigrum), betelvine (P. betle) and Indian long pepper (P. longum) in India and other parts of the world. Occurrence of PYMoV or other badnaviruses in other species of Piper and its variability is not reported so far. We have analysed sequence variability in the conserved putative reverse transcriptase (RT)/ribonuclease H (RNase H) coding region of the virus using specific badnavirus primers from 13 virus isolates of black pepper collected from different cultivars and regions and one isolate each from 23 other species of Piper. Of these, four species failed to produce expected amplicon while amplicon from four other species showed more similarities to plant sequences than to badnaviruses. Of the remaining, isolates from black pepper, P. argyrophyllum, P. attenuatum, P. barberi, P. betle, P. colubrinum, P. galeatum, P. longum, P. ornatum, P. sarmentosum and P. trichostachyon showed an identity of >85 % at the nucleotide and >90 % at the amino acid level with PYMoV indicating that they are isolates of PYMoV. On the other hand high sequence variability of 21-43 % at nucleotide and 17-46 % at amino acid level compared to PYMoV was found among isolates infecting P. bababudani, P. chaba, P. peepuloides, P. mullesua and P. thomsonii suggesting the presence of new badnaviruses. Phylogenetic analyses showed close clustering of all PYMoV isolates that were well separated from other known badnaviruses. This is the first report of occurrence of PYMoV in eight Piper spp and likely occurrence of four new species in five Piper spp.

  15. Larval mosquito communities in discarded vehicle tires in a forested and unforested site: detritus type, amount, and water nutrient differences

    PubMed Central

    Kling, Lindsey J.; Juliano, Steven A.

    2008-01-01

    Discarded tires are an important habitat for larvae of multiple species of disease-transmitting mosquitoes. Although tire locations likely influence composition and abundance of vectors, there are few data linking vector populations to the characteristics of the aquatic tire environment. We sampled water-filled tires at three times at a forested and an unforested site to evaluate how differences in detritus inputs or nutrients in these two macrohabitats may be associated with composition of mosquito-dominated invertebrate communities. The forested site had significantly greater inputs of leaves, twigs, seeds, and fine detritus at the first sampling, but subsequent sampling indicated no differences in inputs of any detritus type. Total phosphorous levels were significantly greater in the forested site, but there was no difference in total nitrogen or total ion concentrations during any sampling. Chlorophyll a levels were not different between sites, even though light levels were greater and canopy cover was less at the unforested site. Culex restuans dominated at the unforested site, and Ochlerotatus triseriatus, Anopheles barberi, and Orthopodomyia signifera were found primarily in the forest. Tires at the forested site had significantly more species but not more individuals than at the unforested site. Leaf amount was a good predictor of densities of Oc. triseriatus and overall abundance of mosquitoes in the forest, whereas the amount of seeds was a good predictor of overall invertebrate richness and of Oc. triseriatus numbers in the unforested site. Differences in mosquito assemblage composition between forested and unforested locations may be explained by greater inputs of plant-based detritus and some nutrients, but other factors, such as macrohabitat or host preferences of adult mosquitoes, also may be important. PMID:18260510

  16. The bumblebees of North China (Apidae, Bombus Latreille).

    PubMed

    An, Jiandong; Huang, Jiaxing; Shao, Youquan; Zhang, Shiwen; Wang, Biao; Liu, Xinyu; Wu, Jie; Williams, Paul H

    2014-07-08

    Bumblebees are important pollinators for wild flowers and agricultural crops. North China is a region of varied geomorphology and vegetation, with plateaus, plains, mountains and deserts, and is part of the greatest hotspot of bumblebee diversity worldwide. We report on a field survey of the bumblebees of North China made between 2005-2012. A sample of 21,636 bumblebee specimens are assigned to 76 species. One older specimen held in London added one more species to this list. Together, these 77 species represent 10 subgenera of the genus Bombus. Seven species are recorded from North China for the first time: B. (St.) distinguendus, B. (Th.) anachoreta, B. (Th.) pseudobaicalensis, B. (Th.) exil, B. (Ps.) campestris, B. (Pr.) infirmus and B. (Ag.) validus. We provide identification keys for both males and females, photographs of the common colour patterns, and distribution maps for all species. We describe variation in local species richness and abundance, and list the food plants used by bumblebees in North China. The most abundant 10 bumblebee species are: B. (Ml.) pyrosoma, B. (Bo.) lantschouensis, B. (Bo.) patagiatus, B. (St.) melanurus, B. (Sb.) sibiricus, B. (Bo.) ignitus, B. (Th.) hedini, B. (Pr.) picipes, B. (Mg.) trifasciatus and B. (Mg.) longipes. Bumblebees are distributed widely within North China, from low elevations near the edge of the North-China plain to high elevations at the edge of the east Qinghai-Tibetan plateau (65-4011 m). The highest species richness is found in meadows of the high elevation east Qinghai-Tibetan plateau and in forests of the Qilianshan mountains in southwestern Gansu. The 337 food plant species recorded here belong to 49 families, showing that bumblebees play an important role in interconnecting agricultural and natural ecosystems in North China. 

  17. Sub-fossil beetle assemblages associated with the "mammoth fauna" in the Late Pleistocene localities of the Ural Mountains and West Siberia.

    PubMed

    Zinovyev, Evgeniy

    2011-01-01

    The distribution of beetles at the end of the Middle Pleninglacial (=terminal Quaternary) was examined based on sub-fossil material from the Ural Mountains and Western Siberia, Russia. All relevant localities of fossil insects have similar radiocarbon dates, ranging between 33,000 and 22,000 C14 years ago. Being situated across the vast territory from the southern Ural Mountains in the South to the middle Yamal Peninsula in the North, they allow latitudinal changes in beetle assemblages of that time to be traced. These beetles lived simultaneously with mammals of the so-called "mammoth fauna" with mammoth, bison, and wooly rhinoceros, the often co-occurring mega-mammalian bones at some of the sites being evidence of this. The beetle assemblages found between 59° and 57°N appear to be the most interesting. Their bulk is referred to as a "mixed" type, one which includes a characteristic combination of arcto-boreal, boreal, steppe and polyzonal species showing no analogues among recent insect complexes. These peculiar faunas seem to have represented a particular zonal type, which disappeared since the end of the Last Glaciation to arrive here with the extinction of the mammoth biota. In contrast, on the sites lying north of 60°N, the beetle communities were similar to modern sub-arctic and arctic faunas, yet with the participation of some sub-boreal steppe components, such as Poecilus ravus Lutshnik and Carabus sibiricus Fischer-Waldheim. This information, when compared with our knowledge of synchronous insect faunas from other regions of northern Eurasia, suggests that the former distribution of beetles in this region could be accounted for both by palaeo-environmental conditions and the impact of grazing by large ruminant mammals across the so-called "mammoth savannas".

  18. Habitat features and predictive habitat modeling for the Colorado chipmunk in southern New Mexico

    USGS Publications Warehouse

    Rivieccio, M.; Thompson, B.C.; Gould, W.R.; Boykin, K.G.

    2003-01-01

    Two subspecies of Colorado chipmunk (state threatened and federal species of concern) occur in southern New Mexico: Tamias quadrivittatus australis in the Organ Mountains and T. q. oscuraensis in the Oscura Mountains. We developed a GIS model of potentially suitable habitat based on vegetation and elevation features, evaluated site classifications of the GIS model, and determined vegetation and terrain features associated with chipmunk occurrence. We compared GIS model classifications with actual vegetation and elevation features measured at 37 sites. At 60 sites we measured 18 habitat variables regarding slope, aspect, tree species, shrub species, and ground cover. We used logistic regression to analyze habitat variables associated with chipmunk presence/absence. All (100%) 37 sample sites (28 predicted suitable, 9 predicted unsuitable) were classified correctly by the GIS model regarding elevation and vegetation. For 28 sites predicted suitable by the GIS model, 18 sites (64%) appeared visually suitable based on habitat variables selected from logistic regression analyses, of which 10 sites (36%) were specifically predicted as suitable habitat via logistic regression. We detected chipmunks at 70% of sites deemed suitable via the logistic regression models. Shrub cover, tree density, plant proximity, presence of logs, and presence of rock outcrop were retained in the logistic model for the Oscura Mountains; litter, shrub cover, and grass cover were retained in the logistic model for the Organ Mountains. Evaluation of predictive models illustrates the need for multi-stage analyses to best judge performance. Microhabitat analyses indicate prospective needs for different management strategies between the subspecies. Sensitivities of each population of the Colorado chipmunk to natural and prescribed fire suggest that partial burnings of areas inhabited by Colorado chipmunks in southern New Mexico may be beneficial. These partial burnings may later help avoid a fire

  19. SEARCH: Spatially Explicit Animal Response to Composition of Habitat.

    PubMed

    Pauli, Benjamin P; McCann, Nicholas P; Zollner, Patrick A; Cummings, Robert; Gilbert, Jonathan H; Gustafson, Eric J

    2013-01-01

    Complex decisions dramatically affect animal dispersal and space use. Dispersing individuals respond to a combination of fine-scale environmental stimuli and internal attributes. Individual-based modeling offers a valuable approach for the investigation of such interactions because it combines the heterogeneity of animal behaviors with spatial detail. Most individual-based models (IBMs), however, vastly oversimplify animal behavior and such behavioral minimalism diminishes the value of these models. We present program SEARCH (Spatially Explicit Animal Response to Composition of Habitat), a spatially explicit, individual-based, population model of animal dispersal through realistic landscapes. SEARCH uses values in Geographic Information System (GIS) maps to apply rules that animals follow during dispersal, thus allowing virtual animals to respond to fine-scale features of the landscape and maintain a detailed memory of areas sensed during movement. SEARCH also incorporates temporally dynamic landscapes so that the environment to which virtual animals respond can change during the course of a simulation. Animals in SEARCH are behaviorally dynamic and able to respond to stimuli based upon their individual experiences. Therefore, SEARCH is able to model behavioral traits of dispersing animals at fine scales and with many dynamic aspects. Such added complexity allows investigation of unique ecological questions. To illustrate SEARCH's capabilities, we simulated case studies using three mammals. We examined the impact of seasonally variable food resources on the weight distribution of dispersing raccoons (Procyon lotor), the effect of temporally dynamic mortality pressure in combination with various levels of behavioral responsiveness in eastern chipmunks (Tamias striatus), and the impact of behavioral plasticity and home range selection on disperser mortality and weight change in virtual American martens (Martes americana). These simulations highlight the relevance of

  20. Genomics and museum specimens.

    PubMed

    Nachman, Michael W

    2013-12-01

    Nearly 25 years ago, Allan Wilson and colleagues isolated DNA sequences from museum specimens of kangaroo rats (Dipodomys panamintinus) and compared these sequences with those from freshly collected animals (Thomas et al. 1990). The museum specimens had been collected up to 78 years earlier, so the two samples provided a direct temporal comparison of patterns of genetic variation. This was not the first time DNA sequences had been isolated from preserved material, but it was the first time it had been carried out with a population sample. Population geneticists often try to make inferences about the influence of historical processes such as selection, drift, mutation and migration on patterns of genetic variation in the present. The work of Wilson and colleagues was important in part because it suggested a way in which population geneticists could actually study genetic change in natural populations through time, much the same way that experimentalists can do with artificial populations in the laboratory. Indeed, the work of Thomas et al. (1990) spawned dozens of studies in which museum specimens were used to compare historical and present-day genetic diversity (reviewed in Wandeler et al. 2007). All of these studies, however, were limited by the same fundamental problem: old DNA is degraded into short fragments. As a consequence, these studies mostly involved PCR amplification of short templates, usually short stretches of mitochondrial DNA or microsatellites. In this issue, Bi et al. (2013) report a breakthrough that should open the door to studies of genomic variation in museum specimens. They used target enrichment (exon capture) and next-generation (Illumina) sequencing to compare patterns of genetic variation in historic and present-day population samples of alpine chipmunks (Tamias alpinus) (Fig. 1). The historic samples came from specimens collected in 1915, so the temporal span of this comparison is nearly 100 years. © 2013 John Wiley & Sons Ltd.

  1. Energetic cost of bot fly parasitism in free-ranging eastern chipmunks.

    PubMed

    Careau, Vincent; Thomas, Donald W; Humphries, Murray M

    2010-02-01

    The energy and nutrient demands of parasites on their hosts are frequently invoked as an explanation for negative impacts of parasitism on host survival and reproductive success. Although cuterebrid bot flies are among the physically largest and most-studied insect parasites of mammals, the only study conducted on metabolic consequences of bot fly parasitism revealed a surprisingly small effect of bot flies on host metabolism. Here we test the prediction that bot fly parasitism increases the resting metabolic rate (RMR) of free-ranging eastern chipmunks (Tamias striatus), particularly in juveniles who have not previously encountered parasites and have to allocate energy to growth. We found no effect of bot fly parasitism on adults. In juveniles, however, we found that RMR strongly increased with the number of bot fly larvae hosted. For a subset of 12 juveniles during a year where parasite prevalence was particularly high, we also compared the RMR before versus during the peak of bot fly prevalence, allowing each individual to act as its own control. Each bot fly larva resulted in a approximately 7.6% increase in the RMR of its host while reducing juvenile growth rates. Finally, bot fly parasitism at the juvenile stage was positively correlated with adult stage RMR, suggesting persistent effects of bot flies on RMR. This study is the first to show an important effect of bot fly parasitism on the metabolism and growth of a wild mammal. Our work highlights the importance of studying cost of parasitism over multiple years in natural settings, as negative effects on hosts are more likely to emerge in periods of high energetic demand (e.g. growing juveniles) and/or in harsh environmental conditions (e.g. low food availability).

  2. Climate impact on the tree growth, vigor and productivity in Siberia

    NASA Astrophysics Data System (ADS)

    Kharuk, V.; Im, S.; Petrov, I.; Dvinskaya, M.

    2017-12-01

    Changing climate has an impact on the Siberian taiga forests. We analyzed GPP and NPP trends, growth index, and stands mortality within the Central Siberia (48°- 75°N/80°-115°E). Considered forests included larch-dominant (Larix sibirica, L. dahurica) and "dark needle conifer" (DNC: Abies sibirica, Pinus sibirica, Picea obovata) stands. GPP and NPP trends calculated based on the Terra/MODIS products. Growth index calculations based on dendrochronology data. Water stress analysis based on the gravimetric and microwave satellite data and MERRA-2 database. Analyzed variables included precipitation, air temperature, VPD, drought index SPEI, and root zone wetness. We found positive GPP trends within majority (>90%) of larch-dominant and DNC ranges, whereas NPP trends are positive on the <40% territory. Negative NPP trends correlated with growth index within key-sites. Siberian pine and fir growth index increase since late 1970th, turning to depression since late 1980th. Within permafrost zone larch growth correlated with sum of positive (t>+10C°) temperatures and vegetation period length. During recent years larch experience water stress in the beginning of vegetation period. Tree decline and mortality observed within DNC stands, and that phenomenon regularly coincided with zones of negative NPP trends. Mortality correlated with VPD, SPEI, and root zone moisture content. Bark beetles (including aggressive species Polygraphus proximus, similar to Dendroctonus ponderosae in American forests) attacked water-stressed trees. Geographically, mortality began on the margins of the DNC range (e.g., within the forest-steppe ecotone) and on terrain features with maximal water stress risk (narrow-shaped hilltops, convex steep south facing slopes, shallow well-drained soils). Currently, Siberian pine and fir decline observed within southern range of these species. In addition, air temperature and aridity increase promotes Siberian silkmoth (Dendrolimus sibiricus) outbreak that

  3. Environmental factors governing population dynamics of rangeland grasshoppers: The first application of GIS and remote sensing to acridology in Russia

    NASA Astrophysics Data System (ADS)

    Latchininsky, Alexandre Vsevolodovich

    Grasshoppers (Orthoptera: Acrididae) are pests of rangeland and crops in temperate Eurasia (Siberia) where landscapes are dominated by short-grass vegetation and have many common features with the prairies of the Great Plains of North America. The zone of economic importance of grasshoppers in Siberia is localized in its southern part between 50° and 55°N and 68° and 132°E. In particular, grasshopper infestations are concentrated in close proximity to Lake Baikal, the world's deepest lake, holding one-fifth of the Earth's total fresh water supply. From a biodiversity perspective, Lake Baikal is unparalleled because >80% of its 1,085 plant and 1,550 animal species are endemic. Broad-scale pesticide applications in the zone close to the Baikal ecosystem can seriously aggravate the hazards of environmental pollution, with potentially catastrophic consequences on a vast scale. Specific composition and density of grasshopper communities were studied over a variety of habitats. Of about 50 local grasshopper species, two gomphocerines, Aeropus sibiricus and Chorthippus albomarginatus, dominated grasshopper communities in dry and mesic habitats, respectively. These species accounted for the most of the crop damage during recent outbreaks in the 1990s requiring large-scale insecticidal control. Annual fluctuations of grasshopper infestations appeared to track changes in air temperature and summer precipitation, but only a synthetic "Aridity index" was statistically significant. Spatial distribution of historic grasshopper infestations was studied using GIS (ERDAS IMAGINERTM) and remote sensing (Landsat TM satellite imagery) and was found to be significantly clumped. The highest grasshopper densities were associated with dry grasslands in transitional zones between foothills and valleys characterized by a particular elevation (600--650 m), soil type (sod-forest, or pararendzina), amount of April--October precipitation (250 mm) and degree of grazing (moderate

  4. Nesting songbirds assess spatial heterogeneity of predatory chipmunks by eavesdropping on their vocalizations.

    PubMed

    Emmering, Quinn C; Schmidt, Kenneth A

    2011-11-01

    1. Information benefits organisms living in a heterogeneous world by reducing uncertainty associated with decision making. For breeding passerines, information reliably associated with nest failure, such as predator activity, can be used to adjust breeding decisions leading to higher reproductive success. 2. Predator vocalizations may provide a source of current information for songbirds to assess spatial heterogeneity in risk that enables them to make appropriate nest-site and territory placement decisions. 3. To determine whether ground-nesting passerines eavesdrop on a common nest predator, the eastern chipmunk (Tamias striatus), we conducted a playback experiment to create spatial heterogeneity in perceived predation risk. We established three types of playback plots broadcasting: (i) chipmunk vocalizations (increased risk), (ii) frog calls (procedural control) and (iii) no playback (silent control). We conducted point counts from plot centres to compare bird activity among treatments and measured the distance of two ground-nesting species' nests, ovenbird (Seiurus aurocapilla) and veery (Catharus fuscescens), from playback stations. 4. Ground-nesting birds significantly reduced their activities up to 30 m from plot centres in response to playbacks of chipmunk calls suggesting an adjustment of territory placement or a reduction of overt behaviours (e.g. singing frequency). In contrast, less vulnerable canopy-nesting species showed no effect across experimental plots. Correspondingly, veeries and ovenbirds nested significantly further from chipmunk playback stations relative to control stations. Interestingly, the magnitude of this response was more than twice as high in ovenbirds than in veeries. 5. Our findings indicate that some breeding passerines may eavesdrop on predator communication, providing an explanation for how some birds assess spatial heterogeneity in predation risk to make breeding site decisions. Thus, heterospecific eavesdropping may

  5. Trypanosoma cruzi reservoir—triatomine vector co-occurrence networks reveal meta-community effects by synanthropic mammals on geographic dispersal

    PubMed Central

    Valiente-Banuet, Leopoldo; Sánchez-Cordero, Víctor; Stephens, Christopher R.

    2017-01-01

    Contemporary patterns of land use and global climate change are modifying regional pools of parasite host species. The impact of host community changes on human disease risk, however, is difficult to assess due to a lack of information about zoonotic parasite host assemblages. We have used a recently developed method to infer parasite-host interactions for Chagas Disease (CD) from vector-host co-occurrence networks. Vector-host networks were constructed to analyze topological characteristics of the network and ecological traits of species’ nodes, which could provide information regarding parasite regional dispersal in Mexico. Twenty-eight triatomine species (vectors) and 396 mammal species (potential hosts) were included using a data-mining approach to develop models to infer most-likely interactions. The final network contained 1,576 links which were analyzed to calculate centrality, connectivity, and modularity. The model predicted links of independently registered Trypanosoma cruzi hosts, which correlated with the degree of parasite-vector co-occurrence. Wiring patterns differed according to node location, while edge density was greater in Neotropical as compared to Nearctic regions. Vectors with greatest public health importance (i.e., Triatoma dimidiata, T. barberi, T. pallidipennis, T. longipennis, etc), did not have stronger links with particular host species, although they had a greater frequency of significant links. In contrast, hosts classified as important based on network properties were synanthropic mammals. The latter were the most common parasite hosts and are likely bridge species between these communities, thereby integrating meta-community scenarios beneficial for long-range parasite dispersal. This was particularly true for rodents, >50% of species are synanthropic and more than 20% have been identified as T. cruzi hosts. In addition to predicting potential host species using the co-occurrence networks, they reveal regions with greater

  6. Phenomenology of pseudotensor mesons and the pseudotensor glueball

    NASA Astrophysics Data System (ADS)

    Koenigstein, Adrian; Giacosa, Francesco

    2016-12-01

    We study the decays of the pseudotensor mesons (π2(1670), K2(1770), η2(1645), η2(1870)) interpreted as the ground-state nonet of 11D2 bar{q}q states using interaction Lagrangians which couple them to pseudoscalar, vector, and tensor mesons. While the decays of π2(1670) and K2(1770) can be well described, the decays of the isoscalar states η2(1645) and η2(1870) can be brought in agreement with the present experimental data only if the mixing angle between nonstrange and strange states is surprisingly large (about -42°, similar to the mixing in the pseudoscalar sector, in which the chiral anomaly is active). Such a large mixing angle is however at odd with all other conventional quark-antiquark nonets: if confirmed, a deeper study of its origin will be needed in the future. Moreover, the bar{q}q assignment of pseudotensor states predicts that the ratio [η2(1870) → a2(1320) π]/[η2(1870) → f2(1270) η] is about 23.5. This value is in agreement with Barberis et al., (20.4 ± 6.6), but disagrees with the recent reanalysis of Anisovich et al., (1.7 ± 0.4). Future experimental studies are necessary to understand this puzzle. If Anisovich's value is confirmed, a simple nonet of pseudoscalar mesons cannot be able to describe data (different assignments and/or additional states, such as an hybrid state, will be needed). In the end, we also evaluate the decays of a pseudoscalar glueball into the aforementioned conventional bar{q}q states: a sizable decay into K^{ast}2(1430) K and a2(1230) π together with a vanishing decay into pseudoscalar-vector pairs (such as ρ(770) π and K^{ast}(892) K) are expected. This information can be helpful in future studies of glueballs at the ongoing BESIII and at the future PANDA experiments.

  7. Field Trial Performance of Herculex XTRA (Cry34Ab1/Cry35Ab1) and SmartStax (Cry34Ab1/Cry35Ab1 + Cry3Bb1) Hybrids and Soil Insecticides Against Western and Northern Corn Rootworms (Coleoptera: Chrysomelidae).

    PubMed

    Johnson, K D; Campbell, L A; Lepping, M D; Rule, D M

    2017-06-01

    Western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), and northern corn rootworm, Diabrotica barberi Smith and Lawrence (Coleoptera: Chrysomelidae), are important insect pests in corn, Zea mays L. For more than a decade, growers have been using transgenic plants expressing proteins from the bacterium Bacillus thuringiensis (Bt) to protect corn roots from feeding. In 2011, western corn rootworm populations were reported to have developed resistance to Bt hybrids expressing Cry3Bb1 and later found to be cross-resistant to hybrids expressing mCry3A and eCry3.1Ab. The identification of resistance to Cry3 (Cry3Bb1, mCry3A, and eCry3.1Ab) hybrids led to concerns about durability and efficacy of products with single traits and of products containing a pyramid of a Cry3 protein and the binary Bt proteins Cry34Ab1 and Cry35Ab1. From 2012 to 2014, 43 field trials were conducted across the central United States to estimate root protection provided by plants expressing Cry34Ab1/Cry35Ab1 alone (Herculex RW) or pyramided with Cry3Bb1 (SmartStax). These technologies were evaluated with and without soil-applied insecticides to determine if additional management measures provided benefit where Cry3 performance was reduced. Trials were categorized for analysis based on rootworm damage levels on Cry3-expressing hybrids and rootworm feeding pressure within each trial. Across scenarios, Cry34Ab1/Cry35Ab1 hybrids provided excellent root protection. Pyramided traits provided greater root and yield protection than non-Bt plus a soil-applied insecticide, and only in trials where larval feeding pressure exceeded two nodes of damage did Cry34Ab1/Cry35Ab1 single-trait hybrids and pyramided hybrids show greater root protection from the addition of soil-applied insecticides. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  8. Forest rodents provide directed dispersal of Jeffrey pine seeds

    USGS Publications Warehouse

    Briggs, J.S.; Wall, S.B.V.; Jenkins, S.H.

    2009-01-01

    Some species of animals provide directed dispersal of plant seeds by transporting them nonrandomly to microsites where their chances of producing healthy seedlings are enhanced. We investigated whether this mutualistic interaction occurs between granivorous rodents and Jeffrey pine (Pinus jeffreyi) in the eastern Sierra Nevada by comparing the effectiveness of random abiotic seed dispersal with the dispersal performed by four species of rodents: deer mice (Peromyscus maniculatus), yellow-pine and long-eared chipmunks (Tamias amoenus and T. quadrimaculatus), and golden-mantled ground squirrels (Spermophilus lateralis). We conducted two caching studies using radio-labeled seeds, the first with individual animals in field enclosures and the second with a community of rodents in open forest. We used artificial caches to compare the fates of seeds placed at the range of microsites and depths used by animals with the fates of seeds dispersed abiotically. Finally, we examined the distribution and survival of naturally establishing seedlings over an eight-year period.Several lines of evidence suggested that this community of rodents provided directed dispersal. Animals preferred to cache seeds in microsites that were favorable for emergence or survival of seedlings and avoided caching in microsites in which seedlings fared worst. Seeds buried at depths typical of animal caches (5–25 mm) produced at least five times more seedlings than did seeds on the forest floor. The four species of rodents differed in the quality of dispersal they provided. Small, shallow caches made by deer mice most resembled seeds dispersed by abiotic processes, whereas many of the large caches made by ground squirrels were buried too deeply for successful emergence of seedlings. Chipmunks made the greatest number of caches within the range of depths and microsites favorable for establishment of pine seedlings. Directed dispersal is an important element of the population dynamics of Jeffrey pine, a

  9. Mechanistic understanding of the effects of natural gas development on sagebrush-obligate songbird nest predation rates

    NASA Astrophysics Data System (ADS)

    Hethcoat, Matthew G.

    Natural gas development has rapidly increased within sagebrush ( Artemisia spp.) dominated landscapes of the Intermountain West. Prior research in the Upper Green River Basin, Wyoming demonstrated increased nest predation of sagebrush-obligate songbirds with higher densities of natural gas wells. To better understand the mechanisms underlying this pattern, I assessed this commonly used index of oil and gas development intensity (well density) for estimating habitat transformation and predicting nest survival for songbirds breeding in energy fields during 2008- 2009 and 2011-2012. We calculated landscape metrics (habitat loss, amount of edge, patch shape complexity, and mean patch size) to identify the aspect of landscape transformation most captured by well density. Well density was most positively associated with the amount of habitat loss within 1 square kilometer. Daily nest survival was relatively invariant with respect to well density for all three species. In contrast, nest survival rates of all three species consistently decreased with increased surrounding habitat loss due to energy development. Thus, although well density and habitat loss were strongly correlated, at times they provided contrasting estimates of nest survival probability. Additionally, we tested the hypothesis that surrounding habitat loss influenced local nest predation rates via increased predator activity. During 2011- 2012, we surveyed predators and monitored songbird nests at twelve sites in western Wyoming. Nine species, representing four mammalian and three avian families, were video-recorded depredating eggs and nestlings. Approximately 75% of depredation events were caused by rodents. While chipmunk (Tamias minimus) detections were negatively associated with increased habitat loss, mice (Peromyscus maniculatus and Reithrodontomys megalotis) and ground squirrels (Ictidomys tridecemlineatus and Urocitellus armatus) increased with greater surrounding habitat loss. Consistent with our

  10. Wood thrush nest success and post-fledging survival across a temporal pulse of small mammal abundance in an oak forest.

    PubMed

    Schmidt, Kenneth A; Rush, Scott A; Ostfeld, Richard S

    2008-07-01

    1. Synchronized mass production of seed crops, such as acorns, produces a resource pulse that may have far-reaching consequences for songbird populations through its effects on avian predators. Seed production in these forests represents only the first of several pulsed events. Secondary pulses emerge as mast-consuming rodents numerically respond to seed production and tertiary pulses emerge as generalist predators, such as raptors, numerically respond to rodents. In turn, these two groups reduce nest productivity and juvenile survivorship 1 and 2 years, respectively, after the initial pulse in seed production. 2. At our study site in south-eastern New York, USA, autumn acorn abundance (primary pulse) largely determines rodent abundance (secondary pulse) the following spring. We tested the hypotheses that the population dynamics of a shrub-nesting passerine (wood thrush Hylocichla mustelina), is influenced by rodents through the: (a) direct effect of predation by rodents; (b) indirect effect of rodents on the abundance of raptors (tertiary pulse); and (c) indirect effect of rodent abundance on raptor diet. The latter specifically hypothesizes that a crash in the rodent population in the wake of region-wide failure of acorn production leads to an extreme diet shift in raptors that increases post-fledging mortality in birds. 3. We conducted a 3-year study to examine variation in wood thrush nest success and fledgling survival, using radio telemetry, across a pulse of rodent abundance (i.e. low, medium and high). We also updated and reanalysed regional wood thrush population growth rates as a function of the annual variation in rodent abundance. 4. Fledgling survivorship, but not nest success, varied in relation to annual rodent abundance. Raptors and eastern chipmunks Tamias striatus were the most commonly identified predators on fledglings. Fledgling survivorship was greatest at intermediate rodent abundance consistent with a shift in raptor diet. Regional rate of

  11. Vegetative filter strips efficiency controlling soil loss and trapping herbicides in two olive orchards at the short-term

    NASA Astrophysics Data System (ADS)

    de Luna, Elena; Guzmán, Gema; Gómez, José A.

    2014-05-01

    , G., Niehaus, E., Passeport, E., Payraudeau, S., Schäfera, R., Tournebize, J., & Schulz, R. (2011). Pesticide risk mitigation by vegetated treatment systems: a meta-analysis. Journal of Environmental Quality, 40: 1068-1080. Vianello, M., Vischetti, C., Scarponi, L., & Zanin, G. (2005). Herbicide losses in runoff events from a field with a low slope: role of a vegetative filter strip. Chemosphere, 61: 717-725. Withers, P. J. A., Hartikainen, H., Barberis, E., Flynn, N. J., & Warren, G. P. (2009). The effect of soil phosphorus on particulate phosphorus in land runoff. European Journal of Soil Science 60: 994-1004.

  12. Possible climate warming effects on vegetation, forests, biotic (insect, pathogene) disturbances and agriculture in Central Siberia for 1960- 2050

    NASA Astrophysics Data System (ADS)

    Tchebakova, N. M.; Parfenova, E. I.; Soja, A. J.; Lysanova, G. I.; Baranchikov, Y. N.; Kuzmina, N. A.

    2012-04-01

    Regional Siberian studies have already registered climate warming over the last half a century (1960-2010). Our analysis showed that winters are already 2-3°C warmer in the north and 1-2°C warmer in the south by 2010. Summer temperatures increased by 1°C in the north and by 1-2°C in the south. Change in precipitation is more complicated, increasing on average 10% in middle latitudes and decreasing 10-20% in the south, promoting local drying in already dry landscapes. Our goal was to summarize results of research we have done for the last decade in the context of climate warming and its consequences for biosystems in Central Siberia. We modeled climate change effects on vegetation shifts, on forest composition and agriculture change, on the insect Siberian moth (Dendrolimus suprans sibiricus Tschetv) and pathogene (Lophodermium pinastri Chev) ranges in Central Siberia for a century (1960-2050) based on historical climate data and GCM-predicted data. Principal results are: In the warmer and drier climate projected by these scenarios, Siberian forests are predicted to decrease and shift northwards and forest-steppe and steppe ecosystems are predicted to dominate over 50% of central Siberia due to the dryer climate by 2080. Permafrost is not predicted to thaw deep enough to sustain dark (Pinus sibirica, Abies sibirica, and Picea obovata) taiga. Over eastern Siberia, larch (Larix dahurica) taiga is predicted to continue to be the dominant zonobiome because of its ability to withstand continuous permafrost. The model also predicts new temperate broadleaf forest and forest-steppe habitats; At least half of central Siberia is predicted to be climatically suitable for agriculture at the end of the century although potential croplands would be limited by the availability of suitable soils agriculture in central Siberia would likely benefit from climate warming Crop production may twofold increase as climate warms during the century; traditional crops (grain, potato

  13. Volcanic risk perception in the Campi Flegrei area

    NASA Astrophysics Data System (ADS)

    Ricci, T.; Barberi, F.; Davis, M. S.; Isaia, R.; Nave, R.

    2013-03-01

    The Campi Flegrei which includes part of the city of Naples, is an active volcanic system; its last eruption occurred in 1538 AD. More recently two significant crises occurred between 1969 and 72 and 1982-84 and were accompanied by ground movements (bradyseism) and seismic activity, forcing people of the town of Pozzuoli to be evacuated. Since 1984 development of a volcanic emergency plan has been underway. In 2000 Civil Protection published a risk map which defined the Red Zone, an area highly at risk from pyroclastic flows, which would need to be evacuated before an eruption. The first study to evaluate the volcanic risk perceptions of the people living within the Campi Flegrei area was completed in spring 2006, resulting in the largest sample ever studied on this topic except for one on Vesuvio area residents by Barberi et al. (2008). A 46 item questionnaire was distributed to 2000 of the approximately 300,000 residents of the Campi Flegrei Red Zone, which includes three towns and four neighborhoods within the city of Naples. A total of 1161 questionnaires were returned, for an overall response rate of 58%. Surveys were distributed to junior high and high school students, as well as to adult members of the general population. Results indicated that unlike issues such as crime, traffic, trash, and unemployment, volcanic hazards are not spontaneously mentioned as a major problem facing their community. However, when asked specific questions about volcanic risks, respondents believe that an eruption is likely and could have serious consequences for themselves and their communities and they are quite worried about the threat. Considering the events of 1969-72 and 1982-84, it was not surprising that respondents indicated earthquakes and ground deformations as more serious threats than eruptive phenomena. Of significant importance is that only 17% of the sample knows about the existence of the Emergency Plan, announced in 2001, and 65% said that they have not received

  14. Exposure pathways and biological receptors: baseline data for the canyon uranium mine, Coconino County, Arizona

    USGS Publications Warehouse

    Hinck, Jo E.; Linder, Greg L.; Darrah, Abigail J.; Drost, Charles A.; Duniway, Michael C.; Johnson, Matthew J.; Méndez-Harclerode, Francisca M.; Nowak, Erika M.; Valdez, Ernest W.; van Riper, Charles; Wolff, S.W.

    2014-01-01

    are the locally endemic Tusayan flameflower Phemeranthus validulus, the long-legged bat Myotis volans, and the Arizona bat Myotis occultus. The most common vertebrate species identified at the mine site included the Mexican spadefoot toad Spea multiplicata, plateau fence lizard Sceloporus tristichus, violetgreen swallow Tachycineta thalassina, pygmy nuthatch Sitta pygmaea, purple martin Progne subis, western bluebird Sialia mexicana, deermouse Peromyscus maniculatus, valley pocket gopher Thomomys bottae, cliff chipmunk Tamias dorsalis, black-tailed jackrabbit Lepus californicus, mule deer Odocoileus hemionus, and elk Cervus canadensis. A limited number of the most common species were collected for contaminant analysis to establish baseline contaminant and radiological concentrations prior to ore extraction. These empirical baseline data will help validate contaminant exposure pathways and potential threats from contaminant exposures to ecological receptors. Resource managers will also be able to use these data to determine the extent to which local species are exposed to chemical and radiation contamination once the mine is operational and producing ore. More broadly, these data could inform resource management decisions on mitigating chemical and radiation exposure of biota at high-grade uranium breccia pipes throughout the Grand Canyon watershed.

  15. Revision of the Ceratocapsine Renodaeus group: Marinonicoris, Pilophoropsis, Renodaeus, and Zanchisme, with descriptions of four new genera (Heteroptera, Miridae, Orthotylinae)

    PubMed Central

    Henry, Thomas J.

    2015-01-01

    and Arizona and New Mexico, USA; Pilophoropsidea keltoni sp. n., from Durango, Mexico; Pilophoropsidea maxima sp. n., from Durango, Mexico; Pilophoropsidea pueblaensis sp. n., from Puebla, Mexico; Pilophoropsidea schaffneri sp. n., from Neuvo León and San Luis Potosi, Mexico; Pilophoropsidea serrata sp. n., from Michoacan, Mexico; Pilophoropsidea touchetae sp. n., from Mexico (Puebla); Pilophoropsidea truncata sp. n., from Mexico (Guerrero); Pilophoropsidea tuberculata sp. n., from Mexico (Guerrero); and Ceratocapsus barberi Knight, comb. n., Ceratocapsus camelus Knight, comb. n. (as the type species of the genus), and Ceratocapsus fascipennis Knight, comb. n. Pilophoropsita gen. n. is described to accommodate Pilophoropsidea schaffneri sp. n. from Costa Rica and Mexico (Jalisco, Nayarit, Oaxaca). The genus Renodaeus Distant is redescribed and the new species Renodaeus mimeticus sp. n. from Ecuador is described. The genus Zanchisme Kirkaldy is reviewed and the four known species are redescribed. Zanchismeopsidea gen. n. is described to accommodate Zanchismeopsidea diegoi sp. n. from Argentina (Santiago del Estero). Provided are habitus illustrations for certain adults (Pilophoropsidea camelus, Pilophoropsis brachyptera Poppius, Renodaeus mimeticus, and Zanchisme mexicanus Carvalho & Schaffner), male and female (when available) color digital images and figures of male genitalia of all species, electron photomicrographs of diagnostic characters for selected species, and keys to the genera and their included species. The taxa treated in this paper are arranged alphabetically by genus and species. PMID:25878535

  16. Tectono-metamorphic evolution and magmatic processes in the thermo-metamorphic aureole of the Monte Capanne pluton (Elba Island, Northern Tyrrhenian Sea, Italy).

    NASA Astrophysics Data System (ADS)

    Morelli, M.; Pandeli, E.; Principi, G.

    2003-04-01

    Introduction In this work we present new structural and petrographic data collected in the thermo-metamorphic aureole of Monte Capanne (western Elba Island) and its metamorphic evolution. In the western Elba Island the Monte Capanne monzogranitic body (ca. 7 Ma) and its thermo-metamorphic aureole crop out. At least two different tectonic units can be distinguished: the Punta Le Tombe Unit, weak re-crystallized, and the Punta Nera Unit. In the latter one the re-crystallization is strong and a pre-intrusion tectono-metamorphic framework is evident (Morelli et al., 2002). The latter is mainly constituted by thermo-metamorphosed meta-ophiolites and meta-sedimentary successions previously correlated by Barberi et al. (1969) with the un-metamorphic ones (Complex IV and V of Trevisan, 1950) cropping out in the central-eastern Elba. According to Perrin (1975) and Reutter &Spohn (1982) a pre-intrusion tectono-metamorphic framework was recognized into such rocks. As suggested by Daniel &Jolivet (1995) complex relationships between metamorphic evolution and magmatic events are also recognizable. Geological Data The Punta Nera Unit crops out all around the Monte Capanne magmatic body and the primary contact with the underlying granitoid is somewhere preserved. This unit, strongly re-crystallized and locally crosscut by aplitic and porphyritic dikes, is represented by (Coli &Pandeli, 1997; Morelli, 2000) tectonized meta-serpentinites, meta-gabbros with rodingitic dikes, rare meta-basalts and meta-ophicalcites, meta-cherts, marbles, cherty meta-limestones, phyllites and meta-limestones with rare meta-arenites intercalations. A "pre-magmatic" tectono-metamorphic framework of this unit is well evident only in its meta-sedimentary portion. The meta-sediments are deformed by syn-metamorphic isoclinal folds caractherized by N-S trending axes, west dipping axial planes and easternward vergence. A later folding and flattening event clearly post-dated the above said folds and associated

  17. PREFACE: International Conference on Computing in High Energy and Nuclear Physics (CHEP 2010)

    NASA Astrophysics Data System (ADS)

    Lin, Simon C.; Shen, Stella; Neufeld, Niko; Gutsche, Oliver; Cattaneo, Marco; Fisk, Ian; Panzer-Steindel, Bernd; Di Meglio, Alberto; Lokajicek, Milos

    2011-12-01

    , as well as two banquets held at the Grand Hotel and Grand Formosa Regent in Taipei. The next CHEP conference will be held in New York, the United States on 21-25 May 2012. We would like to thank the National Science Council of Taiwan, the EU ACEOLE project, commercial sponsors, and the International Advisory Committee and the Programme Committee members for all their support and help. Special thanks to the Programme Committee members for their careful choice of conference contributions and enormous effort in reviewing and editing about 340 post conference proceedings papers. Simon C Lin CHEP 2010 Conference Chair and Proceedings Editor Taipei, Taiwan November 2011 Track Editors/ Programme Committee Chair Simon C Lin, Academia Sinica, Taiwan Online Computing Track Y H Chang, National Central University, Taiwan Harry Cheung, Fermilab, USA Niko Neufeld, CERN, Switzerland Event Processing Track Fabio Cossutti, INFN Trieste, Italy Oliver Gutsche, Fermilab, USA Ryosuke Itoh, KEK, Japan Software Engineering, Data Stores, and Databases Track Marco Cattaneo, CERN, Switzerland Gang Chen, Chinese Academy of Sciences, China Stefan Roiser, CERN, Switzerland Distributed Processing and Analysis Track Kai-Feng Chen, National Taiwan University, Taiwan Ulrik Egede, Imperial College London, UK Ian Fisk, Fermilab, USA Fons Rademakers, CERN, Switzerland Torre Wenaus, BNL, USA Computing Fabrics and Networking Technologies Track Harvey Newman, Caltech, USA Bernd Panzer-Steindel, CERN, Switzerland Antonio Wong, BNL, USA Ian Fisk, Fermilab, USA Niko Neufeld, CERN, Switzerland Grid and Cloud Middleware Track Alberto Di Meglio, CERN, Switzerland Markus Schulz, CERN, Switzerland Collaborative Tools Track Joao Correia Fernandes, CERN, Switzerland Philippe Galvez, Caltech, USA Milos Lokajicek, FZU Prague, Czech Republic International Advisory Committee Chair: Simon C. Lin , Academia Sinica, Taiwan Members: Mohammad Al-Turany , FAIR, Germany Sunanda Banerjee, Fermilab, USA Dario Barberis, CERN