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1

Modelling roach ( Rutilus rutilus ) microhabitat using linear and nonlinear techniques  

Microsoft Academic Search

SUMMARY 1. Multiple linear regression (MLR), generalised additive models (GAM) and artificial neural networks (ANN), were used to define young of the year (0+) roach (Rutilus rutilus) microhabitat and to predict its abundance. 2. 0+ Roach and nine environmental variables were sampled using point abundance sampling by electrofishing in the littoral area of Lake Pareloup (France) during summer 1997. Eight

Sebastien Brosse; Sovan Lek

2000-01-01

2

Behaviour of roach (Rutilus rutilus L.) altered by Ligula intestinalis (Cestoda: Pseudophyllidea): a field demonstration  

Microsoft Academic Search

SUMMARY 1. We studied the influence of a cestode parasite, the tapeworm Ligula intestinalis (L.) on roach (Rutilus rutilus L.) spatial occupancy in a French reservoir (Lake Pareloup, South- west of France). 2. Fish host age, habitat use and parasite occurrence and abundance were determined during a 1 year cycle using monthly gill-net catches. Multivariate analysis (generalized linear models (GLIM)),

GERALDINE L OOT; S EBASTIEN B ROSSE; S OVAN L EK; J EAN-FRANCO I S G U EGAN

3

Impact of habitat fragmentation on genetic population structure of roach, Rutilus rutilus , in a riparian ecosystem  

Microsoft Academic Search

During the last 200 years, the riparianecosystem along major rivers has been reducedto a few scattered remnants. Important elementsof the riparian ecosystem are water bodieswhich were originally connected to the mainriver channel by annual floodings. Due to riverregulations many of these remnants are nowvirtually isolated. In an allozyme analysisusing roach, Rutilus rutilus, as a studyspecies we demonstrate that the geneticdiversity

Bernd Hänfling; W. Durka; R. Brandl

2004-01-01

4

Distribution of nickel in the roach (Rutilus rutilus L. ) after exposure to lethal and sublethal concentrations  

Microsoft Academic Search

In order to evaluate the eventual impact of nickel in fish kills occuring in the river Meuse in Belgium, a series of experiments were performed in which the uptake and distribution of nickel in Rutilus rutilus L., one of the dominant species in the river was studied after administration of nickel alone and in combination with other metals. In decreasing

F. Van Hoof; J. P. Nauwelaers

1984-01-01

5

Cell types in the pituitary of the roach, Rutilus rutilus (L.), (Teleostei).  

PubMed Central

The pituitary of roach, Rutilus rutilus (L.), is pear-shaped and situated in the sella turcica. It is characterised by a large pars nervosa, which penetrates deep into the other regions, splitting the proximal pars distalis. Spatial localisation of nerve fibre types and glandular cell types occurs. Using a variety of staining techniques six glandular cell types have been localised and their distribution is disucssed in relation to those of the other teleost pituitaries.

Jafri, S I; Ensor, D M

1980-01-01

6

Trophic flexibility by roach Rutilus rutilus in novel habitats facilitates rapid growth and invasion success.  

PubMed

Stable isotope and gut content analyses, in conjunction with backcalculated length-at-age estimates of growth, were employed to examine the relationship between trophic ecology and growth rate of a successful invader, Rutilus rutilus, in eight lakes in Ireland. The data revealed that R. rutilus was a trophic generalist in Irish lakes. It utilized a greater proportion of pelagic resources in mesotrophic lakes than in eutrophic lakes, potentially due to a greater density of benthic macroinvertebrates in eutrophic systems. The species was characterized by a large dietary and isotopic niche width and high temporal and spatial variations in diet. Growth rates were typical of those found in the native range of the species and were unrelated to either lake productivity or fish's diet. A generalist trophic ecology confers significant advantages on an invasive species, allowing it to exploit a variety of novel resources and fluctuations in prey availability. PMID:24628030

Hayden, B; Massa-Gallucci, A; Harrod, C; O'grady, M; Caffrey, J; Kelly-Quinn, M

2014-04-01

7

The tapeworm Ligula intestinalis (Cestoda: Pseudophyllidea) inhibits LH expression and puberty in its teleost host, Rutilus rutilus  

Microsoft Academic Search

The tapeworm Ligula intestinalis occurs in the body cavity of its cyprinid second intermediate host, in this study the roach Rutilus rutilus, and inhibits host gonadal development. The mechanism by which infected fish are prevented from reproducing is unknown. Comparison of parameters, such as body length and weight, and condition factor and age, between infected and uninfected individuals, indicated only

V Carter; R Pierce; S Dufour; C Arme; D Hoole

2005-01-01

8

Evidence of altered fertility in female roach (Rutilus rutilus) from the River Seine (France).  

PubMed

A large variety of anthropogenic chemicals present in the aquatic environment have been shown to be able to alter the endocrine system of exposed organisms, potentially impacting their reproductive function. The aim of this study was to assess the effects of environmental pollution on the reproductive system of wild female roach (Rutilus rutilus) from the Seine River (Normandy, France). A suite of biomarkers of endocrine disruption including gonado-somatic index, plasmatic vitellogenin, gonadal aromatase activity and histological parameters (oocyte diameter and gonad maturation) were studied. Female fish from the polluted sites showed a number of reproductive alterations, including inhibited gonad maturation, reduced oocyte growth, reduced levels of plasmatic vitellogenin and 3-fold lower gonadal aromatase activity than females collected in the reference site. Overall, these results highlight the presence of endocrine disruption in female roach from the Seine River. PMID:24811946

Gerbron, M; Geraudie, P; Fernandes, D; Rotchell, J M; Porte, C; Minier, C

2014-08-01

9

Dietary galactooligosaccharide affects intestinal microbiota, stress resistance, and performance of Caspian roach (Rutilus rutilus) fry.  

PubMed

The aim of this study was to assess the effects of galactooligosaccharide (GOS), on the growth performance, stress resistance and intestinal microbiota of Caspian roach (Rutilus rutilus) fry. Specimens (1.36 ± 0.03 g) were fed either a basal control diet (0% GOS, non-supplemented) or the basal diet supplemented with 1% and 2% of GOS. After 7 weeks of the feeding trials, growth factors (final weight, weight gain, specific growth rate, condition factor and food conversion ratio) as well as body composition, resistance to salinity stress and autochthonous intestinal microbiota were assessed. Results demonstrated that at the end of the trial growth factors (final weight, weight gain, SGR, FCR) were significantly higher in 2% GOS fed fish (P < 0.05). In addition, supplementation of GOS significantly increased both survival rate (P < 0.05), and resistance to a salinity stress challenge of prebiotic fed groups (P < 0.05). However, body composition and the total autochthonous intestinal heterotrophic bacteria counts remained unaffected in different treatments (P > 0.05). Nevertheless, autochthonous lactic acid bacteria levels were significantly elevated in fish fed 2% dietary GOS (P < 0.05). These results confirm that GOS improves growth performance, stress resistance and modulates intestinal microbiota by increasing lactic acid bacteria of Caspian roach fry, a very important fish species in the Caspian Sea. PMID:23973845

Hoseinifar, Seyed Hossein; Khalili, Mohsen; Rostami, Hosseinali Khoshbavar; Esteban, M Ángeles

2013-11-01

10

The interactions of abiotic and biotic factors influencing perch Perca fluviatilis and roach Rutilus rutilus populations in small acidified boreal lakes.  

PubMed

Four small, acidified boreal lakes, all sustaining populations of perch Perca fluviatilis, roach Rutilus rutilus and pike Esox lucius, were studied in four successive years. Three lakes were moderately acidified (mean pH of 5·61-5·83), while the fourth was more acidic (mean pH of 5·16) and had a sparse population of R. rutilus. Perca fluviatilis density was higher in this lake (1004 ha(-1)) than in the other three (355-717 ha(-1)), where R. rutilus dominated in terms of numbers (981-2185 ha(-1)). Large, potentially predatory, P. fluviatilis were most abundant in the lake with clearest water, and these seemed to have a negative effect on P. fluviatilis density. Perca fluviatilis mean mass was negatively correlated with R. rutilus biomass and was highest in the most acidic lake with the sparse R. rutilus and the highest P. fluviatilis density. Perca fluviatilis mass correlated positively with pH in two lakes (with the highest fish biomass), suggesting that low pH affected P. fluviatilis mass negatively. Perca fluviatilis growth correlated positively with summer (July to August) air temperature in the lake with sparse R. rutilus, thus differing from P. fluviatilis and R. rutilus growth in the other three lakes. The mean age of P. fluviatilis was generally lower than that of R. rutilus and was lowest in the two lakes with the highest fish biomass, indicating that adult mortality was affected by density-induced factors. PMID:21781101

Linlřkken, A N; Hesthagen, T

2011-08-01

11

The progestin levonorgestrel disrupts gonadotropin expression and sex steroid levels in pubertal roach (Rutilus rutilus).  

PubMed

The aim of the present study was to investigate the effects of the synthetic progestin levonorgestrel (LNG) on the reproductive endocrine system of a teleost fish, the roach (Rutilus rutilus). Pubertal roach were exposed for 28 days in a flow-through system to four concentrations of LNG (3, 31, 312, and 3124ng/l). Both males and females treated with 3124ng/l LNG exhibited the upregulated levels of vitellogenin and oestrogen receptor 1 mRNA in the liver. At the same concentration, LNG caused a significant upregulation of the mRNA expression of the gene encoding luteinising hormone ?-subunit (lh?) and the suppression of the mRNA expression of the gene encoding follicle-stimulating hormone ?-subunit (fsh?) in the pituitary of both male and female roach. A lower LNG concentration (312ng/l) suppressed mRNA expression of fsh? in males only. Females treated with 3124ng/l LNG exhibited significantly lower plasma 11-ketotestosterone (11-KT) and oestradiol (E2) concentrations, whereas their testosterone (T) level was higher compared with the control. Females exposed to 312ng/l LNG presented significantly lower plasma E2 concentrations. Males exposed to ?31ng/l LNG exhibited significantly reduced 11-KT levels. As determined through a histological analysis, the ovaries of females were not affected by LNG exposure, whereas the testes of males exposed to 31 and 312ng/l LNG exhibited a significantly higher percentage of spermatogonia B compared with the control. The results of the present study demonstrate that LNG disrupts the reproductive system of pubertal roach by affecting the pituitary gonadotropin expression and the sex steroid levels. This disruption was determined to occur in males after exposure to an environmentally relevant concentration (31ng/l). Moreover, the highest tested concentration of LNG (3124ng/l) exerted an oestrogenic effect on fish of both sexes. PMID:24893273

Kroupova, H K; Trubiroha, A; Lorenz, C; Contardo-Jara, V; Lutz, I; Grabic, R; Kocour, M; Kloas, W

2014-09-01

12

Filter-feeding in common bream (Abramis brama), white bream (Blicca bjoerkna) and roach (Rutilus rutilus); structures, functions and ecological significance  

Microsoft Academic Search

In this thesis the retention mechanism of the branchial sieve of three sympatric cyprinid fish species, the common bream (Abramis brama), the white bream (Blicca bjoerkna) and the roach (Rutilus rutilus) , is studied. In eutrophic lakes zooplankton is an important food resource and common bream is dominant. Previous research indicated that common bream retains

Berg van den C

1993-01-01

13

Effect of the External Salt Concentration on the Penetration of P³²OâHNaâ through the Branchiae of Rutilus Rutilus L; L\\/INFLUENCE DE LA SALINITE EXTERIEURE SUR LA PENETRATION DE P³²OâHâNa PAR LES BRANCHIES DE RUTILUS RUTILUS L  

Microsoft Academic Search

The uptake of labeled NaHâPOâ by the roach Rutilus rutilus ; L. was studied as a function of the water salinity. After one or two hours in ; solutions with various concentrations the fish were killed, and the activity of ; the blood was determined. The results showed that the phosphate uptake increases ; with increasirg salinity and increasing exposure.

A. E. Pora; A. I. Vertunov

1959-01-01

14

[Analysis of DNA damage/repair level in Rutilus rutilus L. from reservoirs of the Techa River cascade with different levels of radiactive pollution].  

PubMed

The Comet Assay and micronucleus assays have been used to evaluate the condition of the nuclear DNA in erythrocytes of peripheral blood of roach (Rutilus rutilus L.) from water-storage of low-level radioactive waste. The Rutilus rutilus L. from the Shershny reservoir, Chelyabinsk, was used as a control population. Radionuclide maintenance in water, sediments and roach in those reservoirs and Shershny reservoir was defined. The dose rate for Rutilus rutilus L. was calculated using program complex ERICA Assessment Tool 1.0 May 2009. Our investigation has shown that a chronic radiation of population (dose rate - 5.2 mGy/day and 19.5 mGy/day) leads to a significantly higher level of the DNA damage in erythrocytes of peripheral blood and increases the speed of nuclear DNA reparation after irradiation of erythrocytes in vitro. We suppose that it may be a result of the increased quantity of active form of oxygen in cells of the fish in water-storage of low-level radioactive waste. PMID:22690583

Stiazhkina, E V; Obvintseva, N A; Shaposhnikova, I A; Triapitsyna, G A; Stukalov, P M; Priakhin, E A

2012-01-01

15

Brain cytochrome P450 aromatase activity in roach (Rutilus rutilus): seasonal variations and impact of environmental contaminants.  

PubMed

P450 aromatase catalyses the conversion of C19 androgens to C18 estrogens which is thought to be essential for the regulation of the reproductive function. In this study, brain aromatase activity (AA) was measured monthly over a reproductive cycle in wild roach (Rutilus rutilus) sampled in a reference site in Normandy. AA peaked during the breeding season, reaching 35 fmol mg(-1)min(-1) in both male and female fish, and was low during the rest of the year except for a significant rise in October. AA was correlated with ovary maturation (measured either as gonado-somatic index or by histological analysis of the gonads) and plasma sex-steroid levels (11-ketotestosterone in males and 17-?-estradiol in females). Measurements of AA in polluted sites showed that activity was significantly upregulated in sites with fish showing high levels of plasma vitellogenin and large proportion of intersexuality (20-50%) thus suggesting the occurrence of estrogenic compounds and their involvement in AA modulation. PMID:21820384

Geraudie, Perrine; Hinfray, Nathalie; Gerbron, Marie; Porcher, Jean-Marc; Brion, François; Minier, Christophe

2011-10-01

16

Impact of a short-term diazinon exposure on the osmoregulation potentiality of Caspian roach (Rutilus rutilus) fingerlings.  

PubMed

The stocks of Caspian roach (Rutilus rutilus), an economically important species in the Caspian Sea, are depleting. Each year millions of artificially produced fingerlings of this species are restocked in the mouth of rivers of the Southern Caspian Sea (e.g. Qare Soo River), where they are exposed to pesticides originating from regional rice and orchard fields. This early exposure to pesticides could affect the hypo-osmoregulatory ability of juvenile fish. Thus, in this study, Caspian roach fingerlings were exposed to environmentally-relevant concentrations of the organophosphate insecticide diazinon for 96h in fresh water and then transferred to diazinon-free brackish water (BW) for another 96h. We report that cortisol and glucose levels were significantly increased in all diazinon treatments at all sampling time points in comparison to the control group. Moreover, the thyroid hormone levels of TSH, T4, and T3 significantly decreased in diazinon-exposed fish even after the transfer to BW. The electrolytes were differentially affected during the exposure to diazinon and after the transfer to BW. The number of chloride cells in the gill tissue was significantly increased during diazinon exposure at the higher concentrations and decreased to control levels after transfer to BW. Finally, gill and kidney tissues showed many histopathological changes in diazinon-exposed fish even after 240h in BW. These results suggest that the release of Caspian roach fingerlings into the diazinon-contaminated Caspian Sea regions may alter their physiology and jeopardize their survival, which could lead to a failure in rebuilding the Caspian roach stocks in the Caspian Sea. PMID:24630256

Katuli, Kheyrollah Khosravi; Amiri, Bagher Mojazi; Massarsky, Andrey; Yelghi, Saeed

2014-08-01

17

Assessing of heavy metal concentrations in the tissues of Rutilus rutilus caspicus and Neogobius gorlap from Miankaleh international wetland.  

PubMed

The concentrations of four heavy metals (Pb, Cd, Ni and Cr) were measured in tissues of pelagic (Rutilus caspicus) and benthic (Neogobius gorlap) fishes from the Miankaleh international wetland. The maximum concentrations of Pb, Cd, Ni, and Cr (2.06, 1.20, 1.14 and 0.70 ?g g(-1), respectively) were measured in the liver of N. gorlap, while the lowest concentrations were measured in muscle tissue collected from R. caspicus (Pb 0.67; Cd 0.25; Ni 0.21 and Cr 0.08 ?g g(-1)). The mean concentrations of metals in liver of R. caspicus and N. gorlap followed a trend where Pb > Cd > Ni > Cr, whereas in R. caspicus and N. gorlap gills and N. gorlap muscle the following trend was observed Pb > Ni > Cd > Cr. The data of presented study show that the liver and gill tissues have higher metal concentrations than muscle and also the metal concentrations in tissues of N. gorlap (benthic) were higher in comparison with R. caspicus (pelagic). PMID:24064989

Alipour, Hossein; Pourkhabbaz, Alireza; Hassanpour, Mehdi

2013-11-01

18

Normal axial skeleton structure in common roach Rutilus rutilus (Actinopterygii: Cyprinidae) and malformations due to radiation contamination in the area of the Mayak (Chelyabinsk Province, Russia) nuclear plant.  

PubMed

This study was designed to describe normal axial skeletal structure in common roach Rutilus rutilus from putative unaffected environmental conditions, and the occurrence of skeletal malformations in the fish from an area under radiation contamination. Specimens were collected from water bodies of the Techa Cascade Reservoirs located near the Mayak atomic industry plant in the River Ob' drainage, Chelyabinsk Province, Russia. One sample was collected from Lake Irtyash, a reservoir of drinkable water, supplying the town of Ozersk, and the other one from a technical reservoir which is a storage of liquid radioactive waste from Mayak and characterized by high radioactive contamination (mostly (90)Sr and (137)Cs). A comparison was made with historical material collected from the River Ob' before the middle of the 20th century, i.e. before the environment became affected by radioactive contamination. A high number of abnormalities of the axial skeleton were detected in both Mayak samples, in 94 and 97% of examined specimens, in contrast to about 20% in the historical specimens. The abnormalities were in both the unpaired fins and the vertebral column, including the caudal complex and included supernumerary elements, fusions, deformities and displacement of the elements. Most axial skeleton abnormalities, however, were minor, such as splitting, shortening or deformation of spines. Severe defects, such as extensive scolioses, lordoses and kyphoses, were not found. The causes of the abnormalities were not identified in this study, but the high incidence of malformations may be attributed to genetically determined imbalance during development. The almost equal distribution of abnormalities among the fish from non-contaminated and radioactive contaminated reservoirs may be explained by either recent gene flow within the population of R. rutilus in the River Techa system or the effect of unknown unfavourable environmental factors such as chemical pollution. PMID:21967586

Bogutskaya, N G; Zuykov, M A; Naseka, A M; Anderson, E B

2011-10-01

19

[Metazoan parasites of roach Rutilus rutilus (L., 1758), bream Abramis brama (L., 1758) and perch Perca fluviatilis L., 1758 from lakes of river ?yna watershed].  

PubMed

During the years 2004-2006 the parasitological autopsies of three species of fish: roach Rutilus rutilus (L.), bream Abramis brama (L.) and perch Perca fluviatilis L. from three lakes situated in warmi?sko-mazurskie voivodeship were done. The aim of this study was comparable analysis of Metazoan parasite communities which occur in roach, bream and perch in three lakes of river ?yna watershed. These lakes differ by physical (size, depth, eutrophication degree) and biological factors (composition of free-living organisms). The studies showed some relationships between size of fishes and the frequency of some parasite species as well as some relationships in seasonal dynamic of occurrence and maturation of ecto- and endoparasites, which were connected with temperature--main factor regulating processes of fish colonization by parasites and future development of parasites. In quantitative and qualitative analysis of fish parasite community structure the following indexes were used: species richness (S), species diversity of Shannon-Weaver (H'), species diversity of Brillouin (HB) and index of domination Berger-Parker (D). Studies showed that the main indexes of communities structure which indicating on occurring differences between lakes were indexes of diversity component communities and infracommunities and also index of dominance. Communities of monogeneans (roaches and breams) can be use as indicators of water environment. Apart from communities of gills parasites also alfamesotrophic lake ?a?skie and eutrophic Mielno lake were differenced by eyes parasites and allogenic community of bream parasites and by intestinal parasites of roach. PMID:20450014

Kuszta?a, Marzena

2010-01-01

20

Dietary supplementation of fructooligosaccharide (FOS) improves the innate immune response, stress resistance, digestive enzyme activities and growth performance of Caspian roach (Rutilus rutilus) fry.  

PubMed

The present study investigated the effects of prebiotic fructooligosaccharide (FOS) on the innate immune response, stress resistance, digestive enzyme activities, growth factors and survival of Caspian Roach (Rutilus rutilus) fry. After acclimation, fish (0.67 ± 0.03 g) were allocated into 12 tanks (50 fish per tank) and triplicate groups were fed a control diet or diets containing 1%, 2% or 3% FOS. At the end of the trial (7 weeks), humoral innate immune parameters (serum Ig levels, lysozyme activity and alternative complement activity (ACH50)), resistance to salinity stress (150 g L(-1)), digestive enzyme activities (amylase, lipase and protease) and growth factors (final weight, weight gain, specific growth rate (SGR), food conversion ratio (FCR), and condition factor) were assessed. At the end of the study the innate immune responses (Ig levels, lysozyme activity and ACH50) were significantly higher in 2% and 3% FOS fed fish (P < 0.05), whereas, 1% dietary FOS only elevated serum lysozyme activity. All dietary FOS levels significantly increased resistance to a salinity stress challenge (P < 0.05) and highest survival was observed in the 3% FOS group. Similarly, digestive enzyme activities were significantly elevated with increasing levels of dietary FOS (P < 0.05). Subsequently, elevated growth performance (final weight, SGR and FCR) was observed in roach fed 2% and 3% FOS compared to the control group (P < 0.05). These results indicate that FOS can be considered as a beneficial dietary supplement for improving the immune response, stress resistance, digestive enzyme activities and growth performance of Caspian roach fry. PMID:22142706

Soleimani, Narges; Hoseinifar, Seyed Hossein; Merrifield, Daniel L; Barati, Mohsen; Abadi, Zohreh Hassan

2012-02-01

21

Genetic variation and phylogeography of the bank vole ( Clethrionomys glareolus , Arvicolinae, Rodentia) in Russia with special reference to the introgression of the mtDNA of a closely related species, red-backed vole ( Cl. rutilus )  

Microsoft Academic Search

Totally, 294 bank voles (Clethrionomys glareolus) and 18 red-backed voles (Cl. rutilus) from 62 sites of European Russia were studied. Incomplete sequences (967 bp) of the mitochondrial cytochrome b gene were determined for 93 Cl. glareolus individuals from 56 sites and 18 Cl. rutilus individuals from the same habitats. Analysis of the cytochrome b gene variation has demonstrated that practically

N. I. Abramson; E. N. Rodchenkova; A. Yu. Kostygov

2009-01-01

22

Caspian White Fish (Rutilus frisii kutum) as a host for Spring Viraemia of Carp Virus.  

PubMed

Rutilus frisii kutum is a fish of the Cyprinidae Family which is native in Caspian Sea and commercially cultured in Iran. This study was conducted to investigate susceptibility of Caspian White Fish to Spring Viraemia of Carp Virus (SVCV) infection and to evaluate influence of different challenge routes on virulence of the virus. Fingerlings were infected by immersion, intra-peritoneal (i.p.) injection, cohabitation and orally. Dead and surviving fish were collected for histological examination as well as for virus re-isolation by cell culture, Reverse Transcriptase Polymerization Chain Reaction (RT-PCR) and Indirect Fluorescent Antibody Test (IFAT) analysis. The results indicated that immersion was the best infectious route of transmission with the highest mortality, whereas oral transmission showed the lowest mortality. The virus was also re-isolated from dead fish and identified by IFAT. In addition, histopathological changes including branchial, hepatic and splenic necrosis as well as glomerulonephritis and necrosis in kidney were observed in diseased fish tissues but not in the survivors. RT-PCR on samples obtained from surviving fish tissues detected viral genome in the fish surviving from immersion, i.p. injection and cohabitation challenges but not in the fish infected orally. In conclusion, Caspian White Fish are susceptible to infection by SVCV and virulence of the virus could be influenced by route of transmission. In addition, SVCV could persist in surviving fish, which may serve as reservoirs of the virus, transmitting infection to healthy fish population. PMID:24685241

Ghasemi, M; Zamani, H; Hosseini, S M; Haghighi Karsidani, S; Bergmann, S M

2014-06-01

23

Experimental susceptibility of Caspian white fish, Rutilus frisii kutum to Spring viraemia of carp virus.  

PubMed

Caspian white fish (Rutilus frisii kutum) is a fish of the family Cyprinidae, which is commercially harvested from the Caspian Sea. Experimental infection with Spring viraemia of carp virus (SVCV) was conducted in order to examine susceptibility of caspian White Fish and clinical impacts of infection. Fingerling fish were injected intra-peritoneally or waterborne-exposed with SVCV and were monitored daily for 7 weeks. Dead fish and those survived at the end of experimental period were collected for virus isolation and reverse transcriptase polymerase chain reaction analysis. Epithelioma papulosum cyprini cell line was used to re-isolate the virus and indirect fluorescent antibody test was conducted to identify the isolated virus. Infection trials showed that SVCV was highly pathogenic for the Caspian White Fish with mortality rate ranging from 75 to 85 %, depending on the viral challenge model. SVCV genome was detected from dead and apparently healthy fish tissues of both virus exposure models, which showed Caspian White Fish not only can be regarded as a susceptible host, but also serve as a vector of the virus. PMID:24426310

Zamani, H; Ghasemi, M; Hosseini, S M; Haghighi Karsidani, S

2014-01-01

24

Trace element level in different tissues of Rutilus frisii kutum collected from Tajan River, Iran.  

PubMed

Tajan River is among the most significant rivers of the Caspian Sea water basin. In this study, the concentration of Cr, Cu, Fe, Mn, Ni, Pb, Cd, and Zn were determined in brain, heart, liver, gill, bile, and muscle of Rutilus frisii kutum which has great economic value in the Mazandaran state. Trace element levels in fish samples were analyzed by means of atomic absorption spectrometry. Nearly all non-essential metals levels (Ni, Pb, Cd) detected in tissues were higher than limits for fish proposed by FAO/WHO, EU, and TFC. Generally, non-essential metals (Ni, Pb) were so much higher in muscle than the essential metals (Cu, Zn, and Mn) except Fe, which was higher than other metals in nearly all parts, except in gills. Fe distribution pattern in tissues was in order of heart>brain>liver>muscle>bile>gill. Distribution patterns of metal concentrations in the muscle of fish as a main edible part followed the sequence: Fe>Pb>Ni>Cu>Mn>Zn>Cd. PMID:20978865

Eslami, Shahram; Hajizadeh Moghaddam, Akbar; Jafari, Naser; Nabavi, Seyed Fazel; Nabavi, Seyed Mohammad; Ebrahimzadeh, Mohammad Ali

2011-11-01

25

Analysis of nodularin-R in eider (Somateria mollissima), roach (Rutilus rutilus L.), and flounder (Platichthys flesus L.) liver and muscle samples from the western Gulf of Finland, northern Baltic Sea.  

PubMed

Nodularin (NODLN) is a cyanobacterial hepatotoxin that may cause toxic effects at very low exposure levels. The NODLN-producing cyanobacterium Nodularia spumigena forms massive blooms in the northern Baltic Sea, especially during the summer. We analyzed liver and muscle (edible meat) samples from common eider (Somateria mollissima), roach (Rutilus rutilus L.), and flounder (Platichthys flesus L.) for NODLN-R by liquid chromatography/mass spectrometry (LC-MS) and enzyme-linked immunosorbent assay (ELISA). Thirty eiders, 11 roach, and 15 flounders were caught from the western Gulf of Finland between September 2002 and October 2004. Eiders from April to June 2003 were found dead. The majority of samples were analyzed by LC-MS and ELISA from the same sample extracts (water:methanol:n-butanol, 75:20:5, v:v:v). Nodularin was detected in 27 eiders, nine roach, and eight flounders. Eider liver samples contained NODLN up to approximately 200 microg/kg dry weight and muscle samples at approximately 20 microg/kg dry weight, roach liver samples 20 to 900 microg NODLN/kg dry weight and muscle samples 2 to 200 microg NODLN/kg dry weight, and flounder liver samples approximately 5 to 1,100 microg NODLN/kg dry weight and muscle samples up to 100 microg NODLN/kg dry weight. The NODLN concentrations found in individual muscle samples of flounders, eiders, and roach (1-200 microg NODLN/kg dry wt) indicate that screening and risk assessment of NODLN in Baltic Sea edible fish and wildlife are required for the protection of consumer's health. PMID:17089704

Sipiä, Vesa O; Sjövall, Olli; Valtonen, Terhi; Barnaby, Deborah L; Codd, Geoffrey A; Metcalf, James S; Kilpi, Mikael; Mustonen, Olli; Meriluoto, Jussi A O

2006-11-01

26

Effect of copper sulfate on the survival and growth performance of Caspian Sea kutum, Rutilus frisii kutum.  

PubMed

The aim of present study was to determine the LC50/96 h value of copper sulfate and determine the growth performance of Caspian Sea kutum) Rutilus frisii kutum (fingerlings during 60-days sub-lethal copper (Cu). After acclimation period to two weeks, for determine the LC50/96 h value, total of 27 aquarium with a capacity of 60 L each stocked with 10 fishes an aquarium. Tunney four aquarium and 8 concentrations of (Cu) composed the 24 treatments while 3 other aquaria were used as control. For each treatment, three replications were conducted. And experiment to determine the growth performance fish were transferred into fiberglass aquaria of 200 L water capacity for growth trials. The treated fish were kept in the aquarium containing sub-lethal concentrations of Cu (0.11 and 0.23 mg L(-1)) and affected to grow for 60 days, while control fish were placed in metal free water. The results indicated that median lethal concentrations (LC50) of copper to Caspian Sea kutum for 96 h exposure was 2.310 ppm. The chronic sub-lethal water-borne Cu exposure to the fish exerted that fish had significantly decreased final body weight in comparison to control group. The copper sulfate also had significant negative effects on specific growth rate (SGR) and feed conversion efficiency (FCE) in comparison to those fed the control group. The feed conversion ratio (FCR) and condition factor (CF) were significantly increased in comparison with the control group (P?Rutilus frisii kutum). PMID:24133647

Gharedaashi, Esmail; Nekoubin, Hamed; Imanpoor, Mohammad Reza; Taghizadeh, Vahid

2013-01-01

27

[Variations of some physiological and biochemical indices in the population of red-backed vole (Clethrionomys rutilus)].  

PubMed

We studied the changes in the population density of the red-backed vole (Clethrionomys rutilus Pall.) on the northern coast of the Sea of Okhotsk (60 degrees N) during three population cycles (1980-1990). A total of 3111 animals were studied, including 1006 alive voles. For evaluation of the population stress and food deficit, a complex of biochemical (blood level of glucose and liver levels of glycogen and lipids) and physiological (blood content of leucocytes, spleen weight, etc.) indices was used. At a high population density, the influence of stress continues during the reproductive period, after its termination, and, supposedly, until the beginning of a new reproductive period, while at a low population density, the consequences of stress were expressed only in the second half of the reproductive period. The signs of starvation were recorded only in some semiadult voles in a high density population. It has been confirmed that stress acts as a mechanism involved in regulation of the population density of small rodents. PMID:12816070

Cherniavski?, F B; Lazutkin, A N; Mosin, A F

2003-01-01

28

Divergence with gene flow between Ponto-Caspian refugia in an anadromous cyprinid Rutilus frisii revealed by multiple gene phylogeography.  

PubMed

The Black and Caspian Seas have experienced alternating periods of isolation and interconnection over many Milankovitch climate oscillations and most recently became separated when the meltwater overflow from the Caspian Sea ceased at the end of the last glaciation. Climate-induced habitat changes have indisputably had profound impacts on distribution and demography of aquatic species, yet uncertainties remain about the relative roles of isolation and dispersal in the response of species shared between the Black and Caspian Sea basins. We examined these issues using phylogeographical analysis of an anadromous cyprinid fish Rutilus frisii. Bayesian coalescence analyses of sequence variation at two nuclear and one mitochondrial genes suggest that the Black and Caspian Seas supported separate populations of R. frisii during the last glaciation. Parameter estimates from the fitted isolation-with-migration model showed that their separation was not complete, however, and that the two populations continued to exchange genes in both directions. These analyses also suggested that majority of migrations occurred during the Pleistocene, showing that the variation shared between the Black and Caspian Seas is the result of ancient dispersal along the temporary natural connections between the basins, rather than of incomplete lineage sorting or recent human-mediated dispersal. Gene flow between the refugial populations was therefore an important source of genetic variation, and we suggest that it facilitated the evolutionary response of the populations to changing climate. PMID:18261049

Kotlík, Petr; Marková, Silvia; Choleva, Lukás; Bogutskaya, Nina G; Ekmekçi, F Guler; Ivanova, Petya P

2008-02-01

29

[Density-dependent regulation in populations of red-backed voles (Myodes rutilus) in optimal and suboptimal habitats of south-west Siberia].  

PubMed

In a population of red-backed voles (Myodes rutilus) that live in optimal habitats (mountain taiga of North-Eastern Altai) in the years of peak density we have observed total suppression of sexual maturation of young animals which is known to be the main mechanism of density regulation. Increase of voles' local density is accompanied by the increase of glucocorticoids in blood of mature and immature individuals of both sexes that argues for the important role of hypothalamic-pituitary-adrenal axis in density dependent regulation. Another population of red-backed voles from south-west Siberia that live in suboptimal habitats (Novosibirsk city vicinity), had significantly lower density. Here, the dynamics of demographic traits and endocrine status of the individuals did not correlate with interannual fluctuations of abundance and density. Even though, the possibility that in suboptimal conditions local density occasionally reaches the values sufficient for the induction of self-regulation cannot be excluded. PMID:22567967

Novikov, E A; Panov, V V; Moshkin, M P

2012-01-01

30

The effects of dietary xylooligosaccharide on mucosal parameters, intestinal microbiota and morphology and growth performance of Caspian white fish (Rutilus frisii kutum) fry.  

PubMed

The present study investigates the effects of different levels of dietary xylooligosaccharide (XOS) on skin mucus bactericidal activity and protein content, intestinal microbiota and morphology as well as growth performance of Caspian white fish (Rutilus frisii kutum) fry. The specimens (1.54 ± 0.03 g) were fed experimental diets supplemented with different levels of XOS [0% (control), 1%, 2% and 3%] for 8 weeks. The results revealed that feeding on XOS significantly increased skin mucus antibacterial activity and protein levels (P < 0.05) and the highest levels were observed in 3% XOS treatment. The total autochthonous intestinal heterotrophic bacteria significantly increased following XOS administration in diet (P < 0.05). Administration of 2 or 3% XOS in fry diet significantly elevated the autochthonous lactic acid bacteria (P < 0.05). However, dietary XOS had no significant effect on intestinal morphology, growth performance and diet utilisation of Caspian white fish fry (P > 0.05). These results demonstrate beneficial effects of dietary XOS on different parameters of mucosal immunity (both skin mucus and intestinal microbiota) and encourage further studies regards various aspect of XOS administration in early life stages of fish. PMID:24845518

Hoseinifar, Seyed Hossein; Sharifian, Maryam; Vesaghi, Mohammad Javad; Khalili, Mohsen; Esteban, M Ángeles

2014-08-01

31

Reciprocal interactions between roach, Rutilus rutilus, and zooplankton in a small lake: Prey dynamics and fish growth and recruitment  

Microsoft Academic Search

Recruitment success of roach varied dramatically between 1979 and 1982 in Alderfen Broad, a small lake in eastern England. When fry were abundant (in 1979 and 198 1, but not in 1980 or 1982) the summer zooplankton became sparse and was dominated by copepods and rotifers. In years of good recruitment, as each of the preferred cladoceran prey species entered

MARTIN CRYER; GRAEME PEIRSON; COLIN R. TOWNSEND

1986-01-01

32

Biomagnification of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) studied in pike ( Esox lucius), perch ( Perca fluviatilis) and roach ( Rutilus rutilus) from the Baltic Sea  

Microsoft Academic Search

Pike, perch and roach from rural waters of the Baltic Sea were investigated for possible biomagnification of polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). For this we used data on ?15N, weight and sex of the fish. We were able to separate body size effects from trophic position effects on biomagnification. Both these parameters lead to biomagnification of PCBs

Sven Burreau; Yngve Zebühr; Dag Broman; Rasha Ishaq

2004-01-01

33

Brain growth patterns in four European cyprinid fish species (Cyprinidae, Teleostei): roach (Rutilus rutilus), bream (Abramis brama), common carp (Cyprinus carpio) and sabre carp (Pelecus cultratus).  

PubMed

This study compares brain growth in 4 species of cyprinids, each distinctly different in adult brain morphology: roach have generalized brains; bream are characterized by well-developed visual, octavolateralis and gustatory brain regions; common carp show chemosensory (gustatory)-dominated brains, and sabre carp octavolateralis-dominated brains. The growth patterns of 16 regions relative to total brain volume were investigated by computer-aided quantitative histology to illustrate internal brain allometries. In all species the tectum opticum decreases in relative size during growth, whereas the corpus cerebelli increases. In bream and common carp, primary taste centers steadily increase in relative size during growth. In most if not all fish, the brain attains no definite final morphology. Lifelong, growth-related shifts in relative sizes of primary sensory regions may reflect lifelong shifting sensory capabilities. PMID:2379081

Brandstätter, R; Kotrschal, K

1990-01-01

34

Roles of spatial partitioning, competition, and predation in the North American invasion of an exotic mosquito.  

PubMed

Invasion success and species coexistence are often mediated by species interactions across patchily distributed habitats and resources. The invasive mosquito Aedes japonicus japonicus has established in the North American range of the competitively superior resident congener, Aedes albopictus, and the predatory native mosquito Toxorhynchites rutilus. We tested predictions for two hypotheses of invasion success and species coexistence: keystone predation and spatial partitioning. We tested competition between A. japonicus japonicus and A. albopictus with or without T. rutilus in laboratory microcosms, and measured abundances of A. japonicus japonicus, A. albopictus, other resident competing mosquito species, and the presence of T. rutilus among tree holes and tires in metropolitan Washington, DC. In laboratory microcosms, A. albopictus was competitively dominant over A. japonicus japonicus, which is consistent with the few prior studies of competition between these two Aedes species. T. rutilus predation severely lowered performances of both Aedes species but more severely lowered A. japonicus japonicus performance than A. albopictus performance when all three species co-occurred, thus yielding no evidence for keystone predation. Consistent with the spatial partitioning hypothesis, A. japonicus japonicus was negatively correlated and independently aggregated with A. albopictus and all combined resident mosquito competitors and was not associated with T. rutilus among field containers. These results suggest that predation from T. rutilus and competition from A. albopictus are barriers to the spread of A. japonicus japonicus, but that A. japonicus japonicus may escape these interspecific effects by utilizing spatially partitioned container habitats. PMID:24569942

Freed, T Z; Leisnham, P T

2014-06-01

35

An insight into the sialotranscriptome of the non-blood feeding Toxorhynchites amboinensis mosquito  

PubMed Central

All adult mosquitoes take sugar meals, and most adult females also take blood meals to develop eggs. Salivary glands (SG) of males are thus much smaller and do not contain many of the antihemostatic and antiinflammatory compounds found in females. In the past 5 years, transcriptome analyses have identified nearly 70 different genes expressed in adult female SG. For most of these, no function can be assigned in either blood or sugar feeding. Exceptionally, Toxorhynchites mosquitoes are unusual in that they never feed on blood, and the SG of adults are identical in both sexes. Transcriptome analysis of the adult SG of this mosquito was performed to increase knowledge of the evolution of blood feeding—and to identify polypeptide families associated with sugar feeding—in mosquitoes.

Calvo, E.; Pham, V. M.; Ribeiro, J. M. C.

2008-01-01

36

PREDATOR IDENTITY AND ADDITIVE EFFECTS IN A TREEHOLE COMMUNITY  

PubMed Central

Multiple predator species can interact as well as strongly affect lower trophic levels, resulting in complex, nonadditive effects on prey populations and community structure. Studies of aquatic systems have shown that interactive effects of predators on prey are not necessarily predictable from the direct effects of each species alone. To test for complex interactions, the individual and combined effects of a top and intermediate predator on larvae of native and invasive mosquito prey were examined in artificial analogues of water-filled treeholes. The combined effects of the two predators were accurately predicted from single predator treatments by a multiplicative risk model, indicating additivity. Overall survivorship of both prey species decreased greatly in the presence of the top predator Toxorhynchites rutilus. By itself, the intermediate predator Corethrella appendiculata increased survivorship of the native prey species Ochlerotatus triseriatus and decreased survivorship of the invasive prey species Aedes albopictus relative to treatments without predators. Intraguild predation did not occur until alternative prey numbers had been reduced by approximately one-half. Owing to changes in size structure accompanying its growth, T. rutilus consumed more prey as time progressed, whereas C. appendiculata consumed less. The intermediate predator, C. appendiculata, changed species composition by preferentially consuming A. albopictus, while the top predator, T. rutilus, reduced prey density, regardless of species. Although species interactions were in most cases predicted from pairwise interactions, risk reduction from predator interference occurred when C. appendiculata densities were increased and when the predators were similarly sized.

Griswold, Marcus W.; Lounibos, L. Philip

2007-01-01

37

Oral Ingestion of Transgenic RIDL Ae. aegypti Larvae Has No Negative Effect on Two Predator Toxorhynchites Species  

PubMed Central

Dengue is the most important mosquito-borne viral disease. No specific treatment or vaccine is currently available; traditional vector control methods can rarely achieve adequate control. Recently, the RIDL (Release of Insect carrying Dominant Lethality) approach has been developed, based on the sterile insect technique, in which genetically engineered ‘sterile’ homozygous RIDL male insects are released to mate wild females; the offspring inherit a copy of the RIDL construct and die. A RIDL strain of the dengue mosquito, Aedes aegypti, OX513A, expresses a fluorescent marker gene for identification (DsRed2) and a protein (tTAV) that causes the offspring to die. We examined whether these proteins could adversely affect predators that may feed on the insect. Aedes aegypti is a peri-domestic mosquito that typically breeds in small, rain-water-filled containers and has no specific predators. Toxorhynchites larvae feed on small aquatic organisms and are easily reared in the laboratory where they can be fed exclusively on mosquito larvae. To evaluate the effect of a predator feeding on a diet of RIDL insects, OX513A Ae. aegypti larvae were fed to two different species of Toxorhynchites (Tx. splendens and Tx. amboinensis) and effects on life table parameters of all life stages were compared to being fed on wild type larvae. No significant negative effect was observed on any life table parameter studied; this outcome and the benign nature of the expressed proteins (tTAV and DsRed2) indicate that Ae. aegypti OX513A RIDL strain is unlikely to have any adverse effects on predators in the environment.

Nordin, Oreenaiza; Donald, Wesley; Ming, Wong Hong; Ney, Teoh Guat; Mohamed, Khairul Asuad; Halim, Nor Azlina Abdul; Winskill, Peter; Hadi, Azahari Abdul; Muhammad, Zulkamal Safi'in; Lacroix, Renaud; Scaife, Sarah; McKemey, Andrew Robert; Beech, Camilla; Shahnaz, Murad; Alphey, Luke; Nimmo, Derric David; Nazni, Wasi Ahmed; Lee, Han Lim

2013-01-01

38

Influence of container design on predation rate of potential biocontrol agent, Toxorhynchites splendens (Diptera: Culicidae) against dengue vector.  

PubMed

Toxorhynchites splendens larvae are a natural predator of dengue vector mosquito larvae, Aedes albopictus. This study was carried out to evaluate the predation rate of Tx. splendens third instar larvae on Ae. albopictus larvae in 24 h. Each predator was offered prey at a density between 10 to 50 individuals. Predation rate of Tx. splendens were also tested with two manipulated factors; various types of container and different water volumes. The experiment was evaluated in man-made containers (tin cans, plastic drinking glasses and rubber tires) and natural container (bamboo stumps) which were filled with different water volumes (full, half full, 1/4 full, and 1/8 full). The prey density and the characteristics of the container were found as significant factors which influence the predation rate of Tx. splendens. The predator consumed significantly more prey at higher prey densities (40 and 50 preys) compared to the lowest density (10 preys) (F=3.935, df=4, p=0.008). The results showed significantly higher consumption in horizontal shaped container of rubber tire than in vertical shape of bamboo stumps (F=3.100, df=3, p=0.029). However, the water volume had no significant effect on predation rate of Tx. splendens (F=1.736, df=3, p=0.162). We generally suggest that Tx. splendens is best to be released in discarded tires or any other containers with horizontal shape design with wide opening since Tx. splendens can become more effective in searching prey in this type of container design. This predator is also a suitable biocontrol candidates to be introduced either in wet and dry seasons in Malaysia. PMID:24862057

Mohamad, N; Zuharah, W F

2014-03-01

39

Surface charges of the membrane and cell adhesion substances determine the structural integrity of hair bundles from the inner ear of fish  

Microsoft Academic Search

The “hairs” (stereocilia = stereovilli) of sensory cells from the inner ear of vertebrates are interconnected by several types of connectors, whose role is unknown. They appear to stabilize the hair bundle mechanically, and may be directly involved in mechano-electric transduction. Our transmission electron-microscopical investigation of sensory epithelia from two species of fish (Rutilus rutilus, Scardinius erythrophthalmus, both Leuciscidae) has

D. Ch. Neugebauer; U. Thurm

1987-01-01

40

Effect of vertebrate predation on the spatio-temporal distribution of cladocerans in a temperature eutrophic lake  

Microsoft Academic Search

We analysed the spatio-temporal distribution of zooplankton along a profile of 10 stations from the shore to the pelagic zone from April to September 1988, the period when the larvae and juveniles Rutilus rutilus, the most abundant species in the Lake, are in the littoral zone. The digestive tracts of the young roach were analysed. They fed essentially on rotifers

Hassan Taleb; Patricia Reyes-Marchant; Nicole Lair

1994-01-01

41

Tuning host specificity during the ontogeny of a fish ectoparasite: behavioural responses to host-induced cues  

Microsoft Academic Search

The choice between two alternative hosts, brown trout ( Salmo trutta) and roach ( Rutilus rutilus), and the response to visual and olfactory cues were studied in the ontogeny of Argulus coregoni. The initial preference of the smallest parasites for brighter roach changed at the age of 2 weeks, at the size of about 2 mm, for trout, a typical salmonid host.

Victor N. Mikheev; Anna F. Pasternak; E. Tellervo Valtonen

2004-01-01

42

Variations in the spawning periodicity of eight fish species in three English lowland rivers over a 6 year period, inferred from 0+ year fish length distributions  

Microsoft Academic Search

The spawning periodicity of eight fish species was investigated in three English lowland rivers over a 6 year period from patterns in 0ţ year fish standard length (LS) distributions. A single cohort of 0ţ year dace Leuciscus leuciscus, roach Rutilus rutilus and perch Perca fluviatilis was observed each year, suggesting that these species spawned only once annually. By contrast, populations

A. D. Nunn; J. P. Harvey; I. G. Cowx

2007-01-01

43

Three dimensional characteristics of young–of–year pelagic fish schools in lake  

Microsoft Academic Search

Fish schools are aggregative structures encountered in all types of aquatic environments but have as yet been little studied in freshwaters except at small spatial scales. This study represents the three dimensional description of juvenile fish schools (Perca fluviatilis and Rutilus rutilus) in a lake environment using high resolution multibeam sonar system operating at a frequency of 455 kHz, composed

Jean Guillard; Patrice Brehmer; Michel Colon; Yvon Guennégan

2006-01-01

44

Mass spectrometric profiling of glucosamine, glucosamine polymers and their catecholamine adducts. Model reactions and cuticular hydrolysates of Toxorhynchites amboinensis (Culicidae) pupae.  

PubMed

Glucosamine (Gln), glucosamine polymers, and their catecholamine adducts were characterized using positive ion electrospray mass spectrometry (ESMS) and tandem mass spectrometry (ESMS-MS). N-acetyldopamine (NADA), a catecholamine found in many insect cuticles, was oxidized using mushroom tyrosinase, and the resulting quinone derivatives were reacted with Gln, (Gln)3, and polymeric glucosamine (chitosan). Adducts of glucosamine and its trisaccharide with NADA were readily identified as [M + H]+ ions in ESMS spectra, and ESMS-MS of selected ions confirmed the condensation of 1-3 NADA residues with Gln. In addition to Gln modification by the quinone derivatives of NADA, other spectra were consistent with the formation of adducts with N-acetylnoradrenaline and moieties formed by intramolecular cyclization following oxidation. The primary amine of glucosamine was involved in initial adduct formation, but the sites for subsequent additions of oxidized NADA to glucosamine, presumably via hydroxyl groups, could not be identified by ESMS alone. The ESMS spectra of chitosan films infused into the spectrometer following solubilization in acidic methanol/water produced spectra similar to that of (Gln)3 up to m/z 502. Ions of gradually decreasing intensity consistent with (Gln)x, where x = 4-8, were observed. Modification of chitosan films following incubation with NADA plus tyrosinase rendered the films insoluble in dilute acid, simulating the cross-linking process proposed to occur during insect cuticle sclerotization. Acid hydrolysates of the pupal stage of the mosquito Toxorhynchites amboinensis, using only two pupal exuviae for the hydrolyses, were infused into the mass spectrometer without preliminary chromatography. Eight amino acids, glucosamine, N-acetylglucosamine, catecholamines, and a variety of polymers incorporating these compound classes were identified. PMID:10436936

Kerwin, J L; Whitney, D L; Sheikh, A

1999-07-01

45

Biological control of container-breeding mosquitoes, Aedes albopictus and Culex quinquefasciatus, in a Japanese island by release of Toxorhynchites splendens adults.  

PubMed

To control container-breeding mosquitoes in the small island of Minnajima (0.56 km2), northern Okinawa, Japan, laboratory-reared adults (aged 7-10 days) of Toxorhynchites splendens (Palawan strain), a mosquito with predatory larvae, were released repeatedly during 1984, 1986 and 1987. Thirteen species of mosquitoes (Diptera: Culicidae) occurred in artificial containers, ground pools or crab-holes on the island, the predominant species being Aedes (Stegomyia) albopictus and Culex (Culex) quinquefasciatus. Predatory mosquito larvae of Culex (Lutzia) fuscanus and Cx (Lt.) halifaxii were found commonly in wet containers. In the first year of study, during a period of 54 days from 13 May to 5 July 1984, totals of 879 female and 806 male adults of Tx.splendens were released on six occasions. Similarly, between 29 April and 30 August 1986, totals of 2920 female and 2878 male adult Tx.splendens were released. In the third study year, totals of 2041 female and 1783 male Tx.splendens were released on eight occasions during 199 days from 23 April to 7 November 1987. After adult releases at two sites, the immature stages of Tx.splendens were found in 164 out of 502 traps in 1984, 421 out of 933 traps in 1986, and 151 out of 502 traps in 1987. The number of immatures of Tx.splendens present in each trap varied from 1 to 40 in 1984, 1 to 29 in 1986 and 1 to 9 in 1987. Numbers of immatures of the target species found in the traps during August-September averaged 71.9/trap/month in 1984, 114.7/trap/month in 1986 and 36.0/trap/month in 1987, significantly less in the traps with Tx.splendens than in those without them. The present field studies indicated that, in this small island, approximately 250 adult female and 200 male Tx.splendens per month should be released from April to November, and the releases should be carried out every year, in order to control effectively the target mosquitoes Ae.albopictus and Cx quinquefasciatus breeding in artificial containers in Minnajima. PMID:1358271

Miyagi, I; Toma, T; Mogi, M

1992-07-01

46

Local and regional factors influence the structure of treehole metacommunities  

PubMed Central

Background Abiotic and biotic factors in a local habitat may strongly impact the community residing within, but spatially structured metacommunities are also influenced by regional factors such as immigration and colonization. We used three years of monthly treehole census data to evaluate the relative influence of local and regional factors on our study system. Results Every species responded to at least one of three local environmental factors measured: water volume, leaf litter mass, and presence of a top predator. Several species were affected by water volume, and a non-exclusive group of species were influenced by leaf litter mass. Relative abundance of Aedes triseriatus was higher in treeholes with higher volumes of water, and relative abundances of three out of six other species were lower in treeholes with higher volumes of water. Leaf litter mass positively affected densities of Aedes triseriatus and relative abundance of several dipteran species. The density of the top predator, Toxorhynchites rutilus, affected the relative abundance of the two most common species, A. triseriatus and Culicoides guttipennis. Treeholes with T. rutilus had an average of two more species than treeholes without T. rutilus. We found little evidence of synchrony between pairs of treeholes, either spatially or temporally. There were high levels of spatial and temporal turnover, and spatial turnover increased with distance between patches. Conclusion The strong effects of water volume, leaf litter mass, and presence of a top predator, along with the high temporal turnover strongly suggest that species presence and density are determined by local factors and changes in those factors over time. Both low water volume and high predator densities can eliminate populations in local patches, and those populations can recolonize patches when rain refills or predators exit treeholes. Population densities of the same species were not matched between pairs of treeholes, suggesting variation in local factors and limited dispersal. Distance effects on spatial turnover also support limitations to dispersal in the metacommunity, and we conclude that the weight of evidence favors a strong influence of local factors relative to regional factors.

Paradise, Christopher J; Blue, Jarrod D; Burkhart, John Q; Goldberg, Justin; Harshaw, Lauren; Hawkins, Katherine D; Kegan, Benjamin; Krentz, Tyler; Smith, Leslie; Villalpando, Shawn

2008-01-01

47

Effect of boat noise and angling on lake fish behaviour.  

PubMed

The effects of disturbances from recreational activities on the swimming speed and habitat use of roach Rutilus rutilus, perch Perca fluviatilis and pike Esox lucius were explored. Disturbances were applied for 4?h as (1) boating in short intervals with a small outboard internal combustion engine or (2) boating in short intervals combined with angling with artificial lures between engine runs. The response of the fish species was evaluated by high-resolution tracking using an automatic acoustic telemetry system and transmitters with sub-minute burst rates. Rutilus rutilus swimming speed was significantly higher during disturbances [both (1) and (2)] with an immediate reaction shortly after the engine started. Perca fluviatilis displayed increased swimming activity during the first hour of disturbance but not during the following hours. Swimming activity of E. lucius was not significantly different between disturbance periods and the same periods on days without disturbance (control). Rutilus rutilus increased their use of the central part of the lake during disturbances, whereas no habitat change was observed in P. fluviatilis and E. lucius. No difference in fish response was detected between the two types of disturbances (boating with and without angling), indicating that boating was the primary source of disturbance. This study highlights species-specific responses to recreational boating and may have implications for management of human recreational activities in lakes. PMID:24813930

Jacobsen, L; Baktoft, H; Jepsen, N; Aarestrup, K; Berg, S; Skov, C

2014-06-01

48

Immune response in spirlins (Alburnoides bipunctatus, Bloch 1782) infested by Ligula intestinalis parasite.  

PubMed

Ligula intestinalis parasite is a cestode that can cause remarkable damages to fishes. SDS-PAGE is one of the methods that can be used to determine the immune serum band polymorphism and immune responses in fishes infested by Ligula intestinalis. This study reports the results of an investigation conducted using SDS-PAGE focusing on immune serum band polymorphism and on the reaction of the immune system in spirlins (Alburnoides bipunctatus) infested by pleurocercoids of Ligula intestinalis parasite. Serum samples from infested spirlins revealed a polymorphism band which differed from that reported in sera of roaches (Rutilus rutilus), a species of the same Cyprinidae family. PMID:24058932

Halimi, Mostafa; Colagar, Abasalt Hosseinzadeh; Youssefi, Mohammad Reza

2013-01-01

49

Response of animal and vegetative cells to the effect of a typical magnetic storm  

NASA Astrophysics Data System (ADS)

Experimentally reproduced fluctuations of a low-frequency magnetic field in a nanotesla range (magnetic storm) affect the mitosis of animals and vegetative cells. Action of this factor during twenty four hours leads to a significant increase in the proliferative activity of embryo cells in roach ( Rutilus rutilus L.) and meristem cells of onion rootlets ( Allium cepa). The clastogenic effect statistically confirmed only in the Allium test seems to reflect the species specificity of the response and higher sensitivity of the cell association of the onion meristem to magnetic storm.

Talikina, M. G.; Izyumov, Yu. G.; Krylov, V. V.

2013-12-01

50

Antioxidant activity of the mycelium of 21 wild mushroom species  

Microsoft Academic Search

In this study, the antioxidant activity of mycelia from 21 wild mushrooms – Agaricus bresadolanus, Auricularia auricula-judae, Chroogomphus rutilus, Fomes fomentarius, Ganoderma lucidum, Gloeophyllum trabeum, Gymnopus dryophilus, Infundibulicybe geotropa, Inocybe flocculosa var. crocifolia, Inocybe catalaunica, Lentinula edodes, Lentinus sajor-caju, Lycoperdon excipuliforme, Macrolepiota excoriata, Morchella esculenta var. rigida, Morchella intermedia, Omphalotus olearius, Pleurotus djamor, Postia stiptica, Rhizopogon roseolus and Stropharia inuncta

Fatih Kalyoncu; Mustafa Oskay; Hüsniye Kayalar

2010-01-01

51

Morphological and genetic differences among actinosporean stages of fish-parasitic myxosporeans (Myxozoa): difficulties of species identification.  

PubMed

The occurrence and morphology of actinosporean stages of myxosporeans were studied at a fish farm and in the River Tisza in Hungary. The 43 samples sequenced belonged to 10 'genotypes', from which six were determined as new actinosporean types. Based on DNA sequence analysis, the assumed actinosporean developmental stages of four myxozoan species were identified. The raabeia type collected from the worm Branchiura sowerbyi was identified as Myxobolus cultus Yokoyama, Ogawa & Wakabyashi, 1995. This was the first occurrence of this parasite in Hungary. Aurantiactinomyxon type 'A' was assigned as a developmental stage of Thelohanellus hovorkai Achmerov, 1964, triactinomyxon type 'D' was identified as Myxobolus sp. from the fins of roach Rutilus rutilus, while the DNA sequence of the guyenotia type actinosporean was 99.9-100% identical with Sphaerospora sp. from the kidney tubules of goldfish Carassius auratus auratus. Partial 18S rDNA sequences of the myxosporeans Thelohanellus hovorkai, T. nikolskii Achmerov, 1955 and Myxobolus sp. from Rutilus rutilus were new additions to GenBank. The DNA sequence analysis revealed that, in the case of actinosporeans, different 'morphotypes' can belong to the same 'genotype'. This study confirmed that actinospore classification based solely on traditional morphological features may lead to false conclusions, thus sequence analysis of the 18S rDNA and/or other genes is recommended in species and type descriptions. PMID:16676228

Eszterbauer, Edit; Marton, Szilvia; Rácz, Orsolya Z; Letenyei, Márta; Molnár, Kálmán

2006-10-01

52

Genetic distinctiveness of the Korean red-backed vole (Myodes regulus) from Korea, revealed by mitochondrial cytochrome b gene sequences.  

PubMed

To examine the taxonomic status of the Korean red-backed vole (Myodes regulus), the full cytochrome b sequences of 21 red-backed voles from Korea and northeast China were compared with the corresponding haplotypes from 12 species of Myodes and Eothenomys from GenBank. We identified five red-backed voles from Mount Changbai and Harbin as Myodes rufocanus and three from Harbin as M. rutilus, and we confirmed that the red-backed voles from Korea are M. regulus and not Eothenomys regulus. We found that M. regulus from Korea differed from the other five species of Myodes and that the interspecific distances between M. regulus and each of the two species from northeast China were 4.55% (M. rufocanus) and 11.1% (M. rutilus). We concluded that M. regulus is also genetically distinct and is an endemic species of Korea. PMID:21132562

Koh, Hung Sun; Yang, Beong Kug; Heo, Seon Wook; Jang, Kyung Hee; In, Seong Taek

2011-04-01

53

[Infection with opistorchis larvae in the fish family cyprinidae in the Ob-Irtysh River basin in the Tyumen region].  

PubMed

Fishes, such as ide (Leuciscus idus), dace (Leuciscus leuciscus), carpbream (Abramis brama), roach (Rutilus rutilus), and muvarica (Alburnus alburnus), with different frequency and rate of invasion and abundance index were infested with larvae of O. felineus, M. bilis, and P. truncatum. There were the highest rates of fish infection with P. truncatum larvae in the subtaiga zone (the south of the region) and with O. felineus metacercariae in the northern subtaiga and taiga zones. In research, experimental, and clinical studies, the nosological entity opisthorchiasis is a parasitic cenosis consisting of 2-3 co-members requiring their specific identification, which allows therapeutic measures to be more effectively implemented among the population of a hyperendemic focus. PMID:23437717

2012-01-01

54

Contamination Levels and Specific Accumulation of Persistent Organochlorines in Caspian Seal ( Phoca caspica ) from the Caspian Sea, Russia  

Microsoft Academic Search

.   Persistent organochlorines, such as polychlorinated biphenyls (PCBs) including coplanar congeners, DDTs, HCHs, chlordanes\\u000a (CHLs), and HCB, were determined in the blubber of Caspian seals (Phoca caspica) and their fish diet (Rutilus sp.) collected in 1993 from the northern Caspian Sea, Russia. Notable concentrations of DDTs and HCHs were found at mean\\u000a values of 19 and 1.3 ?g\\/g on wet-weight

M. Watanabe; S. Tanabe; R. Tatsukawa; M. Amano; N. Miyazaki; E. A. Petrov; S. L. Khuraskin

1999-01-01

55

Historical biogeography at the crossroads of the northern continents: molecular phylogenetics of red-backed voles (Rodentia: Arvicolinae).  

PubMed

Evolutionary relationships of red-backed voles and their relatives were examined and used to test biogeographic hypotheses. Sequences of the mitochondrial cytochrome b gene were obtained for 25 individuals representing Alticola macrotis, Clethrionomys californicus, C. gapperi, C. glareolus, C. rutilus, and C. rufocanus. These were combined with 21 partial sequences from GenBank for C. regulus, C. rex, C. rufocanus, C. rutilus, Eothenomys imaizumii, E. melanogaster, Phaulomys andersoni, and P. smithii. Complete sequences of three species of Microtus (M. montanus, M. oeconomus, and M. pennsylvanicus), representative species of other arvicoline genera (Myopus, Synaptomys, Arvicola, Ellobius, Ondatra, Lemmus, Dicrostonyx, and Phenacomys), and a sigmodontine representative (Peromyscus) were included as outgroups. We used maximum parsimony, maximum likelihood, distance, and Bayesian based methods and conducted statistical tests on proposed hypotheses of phylogenetic relationships and biogeographic histories. A close relationship of species representing the genera Alticola, Clethrionomys, and Eothenomys was supported (Clethrionomyini); however, the genus Clethrionomys was paraphyletic with respect to both Alticola and Eothenomys. Three major clades were identified as Asian (Eothenomys andersoni, E. smithii, C. rex, C. regulus, and C. rufocanus), Trans-beringian (Alticola macrotis, C. californicus, C. gapperi, C. glarelolus, and C. rutilus), and Taiwanese (E. melanogaster). These results are consistent with the fossil record which indicates an initial diversification in Asia followed by colonization of the Nearctic on at least two occasions. The holarctic species, C. rutilus, appears to have either reinvaded Asia from North America or colonized North America more recently (late Pleistocene) than the two species of Clethrionomys (C. gapperi and C. californicus) that are endemic to North America (early to mid-Pleistocene). Finally, C. gapperi, appears to be comprised of an eastern and a western species, the former with affinities to the Asian C. glareolus and the latter more closely related to C. californicus. PMID:15012954

Cook, Joseph A; Runck, Amy M; Conroy, Chris J

2004-03-01

56

Is there evidence for a shift in fish growth and recruitment success linked to climate change?  

PubMed

This study investigated whether a putative shift in climate regime in the North Atlantic in the 1990s coincided with changes in the growth and recruitment of roach Rutilus rutilus in the north-east of England. The relationships between R. rutilus growth and recruitment and the environment were significantly different before and after the putative shift in climate regime. Water temperature, river discharge, growth, recruitment success and the Gulf Stream Index co-varied until the late 1990s, indicating a gradual progression between periods of warm-and-dry and cold-and-wet summers. Since the late 1990s, there has been an increased prevalence of warm-and-wet summers, and recruitment success has oscillated between extremes on an almost annual basis. The north wall (northern boundary) of the Gulf Stream has been undergoing a displacement south since the late 1990s, and the speed and amplitude of the change appears to support the hypothesis that there was a regime shift in the climate of the North Atlantic Ocean. It is possible that a continued displacement south of the north wall of the Gulf Stream will lead to further increases in river discharge, reductions in water temperature and reduced fish growth and recruitment success in the long term. PMID:21078090

Nunn, A D; Frear, P A; Lee, M; Cowx, I G

2010-11-01

57

Remarks on the seasonal occurrence and identification of young plasmodial stages of Myxobolus spp. infecting cyprinid fishes in Hungary.  

PubMed

During a survey on the Myxobolus infection of two cyprinid fishes, the ide (Leuciscus idus) and the roach (Rutilus rutilus), myxosporean developmental stages were found around the arteries of the gill filaments and in the gill lamellae. An analysis of the 18S rDNA sequences of these stages revealed that plasmodia developing in the ide belonged to Myxobolus elegans, those developing in the gill lamellae of the roach corresponded to M. intimus, while plasmodia developing in close contact with the cartilaginous gill rays proved to be developmental stages of M. feisti. A strict seasonal cycle with a very long intrapiscine development was recorded for M. elegans and M. intimus. Developing plasmodia of the latter Myxobolus spp. occurred from early summer to next spring, and spore formation took place only in April. No seasonality associated with M. feisti infections was found. Developing plasmodia and mature spores of this species occurred simultaneously in different seasons of the year. Myxobolus feisti spore formation always occurred in close contact with the cartilaginous tissue of the gill filaments but spores were rarely encapsulated in the cartilaginous gill rays. PMID:22366133

Molnár, Kálmán; Cech, Gábor; Székely, Csaba

2012-03-01

58

Toxicity of acid aluminium-rich water to seven freshwater fish species: a comparative laboratory study.  

PubMed

The present study focuses on the relative sensitivity among freshwater fish species to aqueous aluminium. Seven common Scandinavian fish species were exposed to acidic Al-rich water, acidic Al-poor water, and approximately neutral water as a control. The relative sensitivity among the species to an acute aluminium challenge was documented, and was in the following order: Atlantic salmon, Salmo salar, as the most sensitive; then roach, Rutilus rutilus; minnow, Phoxinus phoxinus; perch, Perca fluviatilis; grayling, Thymallus thymallus; brown trout, Salmo trutta; and Arctic char, Salvelinus alpinus. Substantial mortality was observed in all species when exposed to the Al-rich medium. Some mortality was also observed in minnow, roach, and brown trout exposed to the acidic Al-poor medium and the control medium. A high resistance to aluminium was observed in Arctic char, while perch was found to be more sensitive to aluminium than expected and, for the first time, a toxic response to aqueous aluminium in grayling was documented. Through controlled experimental studies, the results confirm that aluminium is an important factor in the toxicity of acidified waters to freshwater fish species. PMID:15093412

Poléo, A B; ŘStbye, K; Řxnevad, S A; Andersen, R A; Heibo, E; Vřllestad, L A

1997-01-01

59

Development and validation of a range of endogenous controls to support the implementation of practical Taqman real-time PCR-based surveillance for fish diseases within aquaculture.  

PubMed

The use of Taqman real-time PCR-based technology has recently become more frequent in the detection of pathogens in the aquaculture industry. This interest has necessitated the development of robust and reliable pathogen-detection assays. The development of a range of endogenous control assays to be run alongside these diagnostic assays works to further increase confidence in the latter. This study describes the design of a range of endogenous control assays based on the elongation factor 1-? (EF1-?) gene specific to a range of fish species including Atlantic salmon, Salmo salar; rainbow trout, Oncorhynchus mykiss; brown trout, Salmo trutta; cod, Gadus morhua; haddock, Melanogrammus aeglefinus; saithe, Pollachius virens; whiting, Merlangius merlangus; Norway pout, Trisopterus esmarkii; carp (family Cyprinidae), roach, Rutilus rutilus; European eel, Anguilla anguilla; and herring, Clupea harengus, as well as a number of fish cell lines. Evidence is provided of the validation of these assays for specific species, a range of tissue types and cell lines as well as an example of the potential uses of these assays. PMID:22524565

Bland, F; McIntosh, R; Bain, N; Snow, M

2012-06-01

60

Migration confers survival benefits against avian predators for partially migratory freshwater fish  

PubMed Central

The importance of predation risk in shaping patterns of animal migration is not well studied, mostly owing to difficulties in accurately quantifying predation risk for migratory versus resident individuals. Here, we present data from an extensive field study, which shows that migration in a freshwater fish (roach, Rutilus rutilus) that commonly migrates from lakes to streams during winter confers a significant survival benefit with respect to bird (cormorant, Phalacrocorax carbo spp.) predation. We tagged over 2000 individual fish in two Scandinavian lakes over 4 years and monitored migratory behaviour using passive telemetry. Next, we calculated the predation vulnerability of fish with differing migration strategies, by recovering data from passive integrated transponder tags of fish eaten by cormorants at communal roosts close to the lakes. We show that fish can reduce their predation risk from cormorants by migrating into streams, and that probability of being preyed upon by cormorants is positively related to the time individuals spend in the lake during winter. Our data add to the growing body of evidence that highlights the importance of predation for migratory dynamics, and, to our knowledge, is one of the first studies to directly quantify a predator avoidance benefit to migrants in the field.

Skov, Christian; Chapman, Ben B.; Baktoft, Henrik; Brodersen, Jakob; Bronmark, Christer; Hansson, Lars-Anders; Hulthen, Kaj; Nilsson, P. Anders

2013-01-01

61

Biology of Amur sleeper (Perccottus glehni) in the Delta of the Selenga River, Buryatia, Russia  

USGS Publications Warehouse

We determined the fecundity, growth, diet, and density of the Amur sleeper (Perccottus glehni) in the Selenga River Delta on Lake Baikal during 1986-1991 to better understand how this invading exotic will affect Baikal's endemic fishes. We also compared the Amur sleeper's diet with that of other fishes living in the delta. The largest Amur sleepers were about 200 mm long and weighed 200 g; the oldest were age 7. All females were mature at age 2. Fecundity ranged from 884 eggs at age 1 to 37,056 eggs at age 7. Highest densities of Amur sleepers were found in oxbow lakes where densities sometimes exceeded 4,000 fish per ha. The bulk of the diet of Amur sleeper age 2 and older was chironomids, fish, and fish eggs. Chironomids were also important in the diet of the commercially valuable Siberian roach (Rutilus rutilus lacustris) and Siberian dace (Leuciscus leuciscus baicalensis). Thus the Amur sleeper may cause population declines of these important endemic fishes through resource competition and predation on their juvenile life stages. However, Amur sleepers were the species of fish most frequently eaten by Eurasian perch (Perca fluviatilis) and northern pike (Esox lucius). So, maintaining vigorous populations of these two predators may well be an effective strategy for limiting the size of Amur sleeper populations.

Litvinov, Alexander G.; O'Gorman, Robert

1996-01-01

62

Catalasic activity in fish liver: improvement of the UV to visible analytic method.  

PubMed

Antioxidative defenses and more especially catalasic activity (CAT) are studied in a large range of scientific research thematics. In environmental sciences, the problematic of oxidative stress is of great interest as pollutants can induce perturbations of redox homeostasis. Consequently, changes in antioxidative defenses levels in fish tissues and particularly in liver are used as potential biomarkers of pollution. In most studies, the CAT was assayed by following during 5 min the consumption of H2O2 in cytosolic buffered extracts at 240 nm (UV-method). This study proposed a development of this method in the visible, using permanganate and a 525-nm detection, which was more accurate, sensitive, and rapid. Moreover, the hepatic CAT of six different fish species [a cyclidae (Nimbochromis linni), 3 cyprinidae (Brachydanio rerio, Rutilus rutilus, Cyprinus carpio), an anguillidae (Anguilla anguilla), and a percidae (Perca fluviatilus)] was evaluated with the two protocols (UV- and KMnO4-method). The results but also the thermal optimum of the reaction and the interest of CAT as biomarker in ecotoxicology were discussed. PMID:23224832

Paris-Palacios, Séverine; Delahaut, Laurence; Carreras, Alexis; Thomas, Marielle; Biagianti-Risbourg, Sylvie

2013-08-01

63

The xenometabolome and novel contaminant markers in fish exposed to a wastewater treatment works effluent.  

PubMed

Organisms exposed to wastewater treatment works (WwTW) effluents accumulate complex mixtures of xenobiotics but there is a scarcity of information on the nature and impacts of these chemical mixtures. We applied metabolomics techniques as a novel approach to identify xenobiotics and their metabolites (the xenometabolome) that bioconcentrate in fish exposed to a WwTW effluent. Exposed juvenile rainbow trout (Oncorhynchus mykiss) accumulated surfactants, naphthols, chlorinated xylenols, and phenoxyphenols, chlorophenes, resin acids, mefenamic acid, oxybenzone, and steroidal alkaloids in the bile or plasma, and there were perturbations in the plasma concentrations of bile acids and lipids. Exposure of adult roach (Rutilus rutilus) to 50% or 100% concentrations of the same effluent resulted in dose-dependent increases in plasma concentrations of xenometabolites as well as cyprinol sulfate and taurocholic acid, lysophospholipids, and a decrease in sphingosine levels (a key component of cell membrane lipids). Our findings reveal the highly complex nature of xenobiotics accumulating in effluent-exposed fish, and the great potential of metabolomics for both identifying plasma marker (bio)chemicals for monitoring exposure to wastewater effluents, and for targeting studies on potential consequent impacts on fish health. PMID:22803593

Al-Salhi, Raghad; Abdul-Sada, Alaa; Lange, Anke; Tyler, Charles R; Hill, Elizabeth M

2012-08-21

64

Spatial distribution of brood-bearing females of limnetic species of Cladocera.  

PubMed

In this study, the spatial distribution of brood-bearing females of five species of limnetic cladocerans (Daphnia cucullata, D. longispina, Bosmina coregoni, B. longirostris, Diaphanosoma brachyurum) in the deep mesotrophic lake in relation to the predation pressure of planktivorous fish (roach Rutilus rutilus, perch Perca fluviatilis, catfish Ictalurus nebulosus, white fish Coregonus albula, bleak Alburnus alburnus), and planktonic invertebrates (cyclopoids Mesocyclops leuckartii, Thermocyclops oithonoides, T. crassus, and cladoceran Leptodora kindtii) as well as some environmental variables was estimated. Most cladocerans showed apparent differences in horizontal distribution (ANOVA F=0.2-0.45, P<0.05) in the littoral zone and lack of such differences in the pelagic zone (F=0.07-0.13, P>0.05). Vertical distribution of most species, in turn, showed a clear pattern in the pelagic zone (F=0.31-0.39, P<0.05) and less regularities in the littoral zone (F=0.15-029, P>0.05). The differences in spatial distribution of non-predated and predated species suggest that predation pressure, but not predatory type, was an important factor structuring their distribution. Other factors that affected their distribution were conductivity, dissolved oxygen, TOC and macrophyte biomass; however, most of those variables better explained the distribution of brood-bearing cladocerans in the vertical than horizontal aspect. PMID:24161243

Adamczuk, Ma?gorzata; Mieczan, Tomasz

2013-09-01

65

Roach, Sex, and Gender-Bending Chemicals: The Feminization of Wild Fish in English Rivers  

NSDL National Science Digital Library

Feminization of the male roach, Rutilus rutilus, a freshwater, group-spawning fish, is widespread in English rivers; among the causative agents are natural and synthetic steroidal estrogens and chemicals that mimic estrogens. In feminized male roach, concentrations of the egg-yolk protein vitellogenin are elevated, sex steroid hormone dynamics are altered, and gonad development is disrupted (most notably, a female reproductive duct or developing eggs [oocytes] are present in the testis). In some English rivers containing high levels of estrogens, all male roach sampled have been feminized to varying degrees. In the more severely affected males, individuals produce low-quality sperm with a reduced capability for fertilization. Laboratory studies have shown that the environmental estrogens responsible for inducing gonadal feminization in roach can also alter reproductive behavior, disrupting normal breeding dynamics (parentage) in the zebrafish, another group-spawning fish. Together these findings indicate that feminization of wild roach may result in adverse population-level effects, but this hypothesis has yet to be fully addressed.

Charles Tyler, R. (University of Exeter - UK;); Susan Jobling (Brunel University - UK;)

2008-12-01

66

Distribution and ecology of Dreissena polymorpha (pallas) and Dreissena bugensis (andrusov) in the upper Volga basin  

USGS Publications Warehouse

This paper presents data on contemporary distribution patterns of two species of Dreissenidae, the zebra mussel (Dreissena polymorpha) and the Quagga mussel (Dreissena bugensis), and their role in ecosystem processes in the Ivan'kovo, Uglich, Rybinsk, and Gorky Reservoirs of the Upper Volga River basin. The role of zebra mussel was also studied in experimental mesocosms of 15 m3. Maximum abundance and species diversity of macroinvertebrates, especially of leeches, polychaetes, crustaceans, and heterotopic insects, were attained in the portions of reservoirs where Dreissenidae were present and in experimental mesocosms where zebra mussel biomass was the highest. In the mesocosm studies, the presence of zebra mussel druses (colonies) provided shelter for macroinvertebrates, reducing their vulnerability to predation by perch (Perca fluviatills) larvae and yearlings, thereby increasing macroinvertebrate species diversity. It was shown that in addition to its role in aquatic biocenosis (ecological community) formation and water purification, Dreissenidae are important food objects for benthophagous fishes, especially roach (Rutilus rutilus). Examination of intestines of benthophagous fishes showed that the length of Dreissenidae ranged from 5 to 20 mm in roach; from 4 to 14 mm in silver bream (Blicca bjoerkna), and from 2 to 10 mm in bream (Abramis brama). The largest mussels consumed were Quagga mussels up to 30 mm, noted in the predatory cyprinid, ide (Leuciscus idus). Copyright ?? 2006 by ASTM International.

Shcherbina, G. Kh.; Buckler, D. R.

2006-01-01

67

Toxic effects of mining effluents on fish gills in a subarctic lake system in NW Russia.  

PubMed

The mining company Karelian Pellet in northwestern Russia extracts iron ore and processes it locally into pellets. The production operations affect the environment in the form of air pollution and wastewater emissions to lakes downstream from the factory. The toxic effects of the mining effluents on gills of perch (Perca fluviatilis) and roach (Rutilus rutilus) were studied. In lakes close to the factory, heavy metal concentrations in gills were not high, even though the metal content in sediment was elevated. In fish gills the relative proportion of phosphatidylcholine was elevated and cholesterol reduced, while the histological structure of the gills was changed. The number of mucus cells, as well as the sizes and the lengths of open areas in the chloride cells, had increased in spring and summer. The hypertrophy of chloride cells is possibly caused by the increased ambient concentrations of K+ and Li+. Changes in gill cholesterol and phospholipid proportions increase the fluidity of membranes and possibly strengthen their protective qualities, counterbalancing the adverse changes in chloride cell structure. The bioavailability and toxic effects of metals on fish are reduced by the hardness and high pH of water discharged by the mining plant. PMID:15041251

Tkatcheva, Victoria; Hyvärinen, Heikki; Kukkonen, Jussi; Ryzhkov, Leonid P; Holopainen, Ismo J

2004-03-01

68

First detection of circovirus-like sequences in amphibians and novel putative circoviruses in fishes.  

PubMed

The negative samples of a collection, established originally for seeking new adeno- and herpesviruses in lower vertebrates, were screened for the pres-ence of circoviruses by a consensus nested PCR targeting the gene coding for the replication-associated protein. Six fish samples representing five species, namely asp (Aspius aspius), roach (Rutilus rutilus), common bream (Abramis brama), round goby (Neogobius melanostomus) and monkey goby (Neogobius fluviatilis), as well as three frog samples were found positive for circoviral DNA. Sequence analysis of the amplicons indicated the presence of three novel putative circo-like viruses and a circovirus in Hungarian fishes and one novel circovirus in a common toad (Bufo bufo), and another one in a dead and an alive specimen of green tree frog (Litoria caerulea), respectively. In phylogeny reconstruction, the putative bream circovirus clustered together with circoviruses discovered in other cyprinid fishes recently. Three other piscine circoviral sequences appeared closest to sequences derived from different environmental samples. Surprisingly, the nucleotide sequence derived from two fish samples (a bream and a monkey goby) proved to be from porcine circovirus 2 (PCV2), almost identical to a sequence detected in Sweden previously. This is the first report on the detection of PCV2 in fish and circoviral DNA in amphibian hosts. PMID:24334078

Tarján, Zoltán László; Pénzes, Judit J; Tóth, Róza P; Benk?, Mária

2014-03-01

69

Interactions between predation and resources shape zooplankton population dynamics.  

PubMed

Identifying the relative importance of predation and resources in population dynamics has a long tradition in ecology, while interactions between them have been studied less intensively. In order to disentangle the effects of predation by juvenile fish, algal resource availability and their interactive effects on zooplankton population dynamics, we conducted an enclosure experiment where zooplankton were exposed to a gradient of predation of roach (Rutilus rutilus) at different algal concentrations. We show that zooplankton populations collapse under high predation pressure irrespective of resource availability, confirming that juvenile fish are able to severely reduce zooplankton prey when occurring in high densities. At lower predation pressure, however, the effect of predation depended on algal resource availability since high algal resource supply buffered against predation. Hence, we suggest that interactions between mass-hatching of fish, and the strong fluctuations in algal resources in spring have the potential to regulate zooplankton population dynamics. In a broader perspective, increasing spring temperatures due to global warming will most likely affect the timing of these processes and have consequences for the spring and summer zooplankton dynamics. PMID:21304980

Nicolle, Alice; Hansson, Lars-Anders; Brodersen, Jakob; Nilsson, P Anders; Brönmark, Christer

2011-01-01

70

The Consequences of Feminization in Breeding Groups of Wild Fish  

PubMed Central

Background The feminization of nature by endocrine-disrupting chemicals (EDCs) is a key environmental issue affecting both terrestrial and aquatic wildlife. A crucial and as yet unanswered question is whether EDCs have adverse impacts on the sustainability of wildlife populations. There is widespread concern that intersex fish are reproductively compromised, with potential population-level consequences. However, to date, only in vitro sperm quality data are available in support of this hypothesis. Objective The aim of this study was to examine whether wild endocrine-disrupted fish can compete successfully in a realistic breeding scenario. Methods In two competitive breeding experiments using wild roach (Rutilus rutilus), we used DNA microsatellites to assign parentage and thus determine reproductive success of the adults. Results In both studies, the majority of intersex fish were able to breed, albeit with varying degrees of success. In the first study, where most intersex fish were only mildly feminized, body length was the only factor correlated with reproductive success. In the second study, which included a higher number of more severely intersex fish, reproductive performance was negatively correlated with severity of intersex. The intersex condition reduced reproductive performance by up to 76% for the most feminized individuals in this study, demonstrating a significant adverse effect of intersex on reproductive performance. Conclusion Feminization of male fish is likely to be an important determinant of reproductive performance in rivers where there is a high prevalence of moderately to severely feminized males.

Harris, Catherine A.; Hamilton, Patrick B.; Runnalls, Tamsin J.; Vinciotti, Veronica; Henshaw, Alan; Hodgson, Dave; Coe, Tobias S.; Jobling, Susan; Tyler, Charles R.; Sumpter, John P.

2011-01-01

71

Use Carum copticum essential oil for controlling the Listeria monocytogenes growth in fish model system  

PubMed Central

This study was conducted to evaluate the antibacterial effect of Carum copticum essential oil (Ajowan EO) against Listeria monocytogenes in fish model system. Ajowan EO chemical composition was determined by gas chromatography/mass spectral analysis and the highest concentration of Carum copticum essential oil without any significant changes on sensory properties of kutum fish (Rutilus frisii kutum) was assigned. Then the inhibitory effect of Ajowan EO at different concentrations in presence of salt and smoke component was tested on L. monocytogenes growth in fish peptone broth (FPB), kutum broth and cold smoked kutum broth at 4 °C for 12 days. Ajowan EO completely decreased the number of L. monocytogenes in FPB after 12 days of storage, however, antimicrobial effect of EO significantly reduced in kutum and cold smoked kutum broth. Addition of 4% NaCl and smoke component improved the anti-listerial activity of Ajowan EO in all fish model broths.

Rabiey, Soghra; Hosseini, Hedayat; Rezaei, Masoud

2014-01-01

72

Occurrence and histological response of Raphidascaris acus (Nematoda: Ascaridoidea) in roach from four lakes differing in water quality.  

PubMed

Seasonality and size-related infection of Raphidascaris acus larvae from the inner organs and intestine of roach (Rutilus rutilus) were studied in 4 lakes of differing water quality and pollution level in Central Finland between August 1985 and November 1986. The influence of R. acus larvae on the liver and pancreatic tissues of roach was examined histologically in additional material from 1989 and 1990. The inner organs of roach were most heavily infected with R. acus in the eutrophic, polluted Lake Vatia (63% of fish infected with 4.0 nematodes/fish) and in the two eutrophic lakes, compared to the oligotrophic Lake Peurunka (23%, 0.8). The prevalence of free R. acus larvae in the intestine of roach was almost as high but the intensity only about half of that found in the inner organs. The prevalence of infection had significantly higher values in autumn in most cases, and larvae accumulated in the inner organs and intestine of older roach. In histological studies it was found that larvae occurred more often in the pancreatic tissue than in the liver, but in both organs the majority of the larvae were dead and partly destroyed. The most typical host response against R. acus was a chronic granulomatous inflammatory reaction. Granulomas and developing granulomas containing worms at different stages of degeneration are described; they were found in all of the lakes studied throughout the year and also in one and the same fish. On average only 37 and 21% of the worms in the liver and pancreas, respectively, were alive. No obvious difference in the histological response against R. acus was noted between the lakes. PMID:8026896

Valtonen, E T; Haaparanta, A; Hoffmann, R W

1994-04-01

73

Karyotypes, male meiosis and comparative FISH mapping of 18S ribosomal DNA and telomeric (TTAGG) n repeat in eight species of true bugs (Hemiptera, Heteroptera)  

PubMed Central

Abstract Eight species belonging to five true bug families were analyzed using DAPI/CMA3-staining and fluorescence in situ hybridization (FISH) with telomeric (TTAGG)n and 18S rDNA probes. Standard chromosomal complements are reported for the first time for Deraeocoris rutilus (Herrich-Schäffer, 1838) (2n=30+2m+XY) and Deraeocoris ruber(Linnaeus, 1758) (2n=30+2m+XY) from the family Miridae. Using FISH, the location of a 18S rDNA cluster was detected in these species and in five more species: Megaloceroea recticornis (Geoffroy, 1785) (2n=30+XY) from the Miridae; Oxycarenus lavaterae (Fabricius, 1787) (2n=14+2m+XY) from the Lygaeidae s.l.; Pyrrhocoris apterus (Linnaeus, 1758) (2n=22+X) from the Pyrrhocoridae; Eurydema oleracea (Linnaeus, 1758) (2n=12+XY) and Graphosoma lineatum (Linnaeus, 1758) (2n=12+XY) from the Pentatomidae. The species were found to differ with respect to location of a 18S rRNA gene cluster which resides on autosomes in Oxycarenus lavaterae and Pyrrhocoris apterus, whereas it locates on sex chromosomes in other five species. The 18S rDNA location provides the ?rst physical landmark of the genomes of the species studied. The insect consensus telomeric pentanucleotide (TTAGG)n was demonstrated to be absent in all the species studied in this respect, Deraeocoris rutilus, Megaloceroea recticornis, Cimex lectularius Linnaeus, 1758 (Cimicidae), Eurydema oleracea, and Graphosoma lineatum, supporting the hypothesis that this motif was lost in early evolution of the Heteroptera and secondarily replaced with another motif (yet unknown) or the alternative telomerase-independent mechanisms of telomere maintenance. Dot-blot hybridization analysis of the genomic DNA from Cimex lectularius, Nabis sp. and Oxycarenus lavaterae with (TTAGG)n and six other telomeric probes likewise provided a negative result.

Grozeva, S.; Kuznetsova, V.G.; Anokhin, B.A.

2011-01-01

74

Health Effects in Fish of Long-Term Exposure to Effluents from Wastewater Treatment Works  

PubMed Central

Concern has been raised in recent years that exposure to wastewater treatment effluents containing estrogenic chemicals can disrupt the endocrine functioning of riverine fish and cause permanent alterations in the structure and function of the reproductive system. Reproductive disorders may not necessarily arise as a result of estrogenic effects alone, and there is a need for a better understanding of the relative importance of endocrine disruption in relation to other forms of toxicity. Here, the integrated health effects of long-term effluent exposure are reported (reproductive, endocrine, immune, genotoxic, nephrotoxic). Early life-stage roach, Rutilus rutilus, were exposed for 300 days to treated wastewater effluent at concentrations of 0, 15.2, 34.8, and 78.7% (with dechlorinated tap water as diluent). Concentrations of treated effluents that induced feminization of male roach, measured as vitellogenin induction and histological alteration to gonads, also caused statistically significant alterations in kidney development (tubule diameter), modulated immune function (differential cell count, total number of thrombocytes), and caused genotoxic damage (micronucleus induction and single-strand breaks in gill and blood cells). Genotoxic and immunotoxic effects occurred at concentrations of wastewater effluent lower than those required to induce recognizable changes in the structure and function of the reproductive endocrine system. These findings emphasize the need for multiple biological end points in tests that assess the potential health effects of wastewater effluents. They also suggest that for some effluents, genotoxic and immune end points may be more sensitive than estrogenic (endocrine-mediated) end points as indicators of exposure in fish.

Liney, Katherine E.; Hagger, Josephine A.; Tyler, Charles R.; Depledge, Michael H.; Galloway, Tamara S.; Jobling, Susan

2006-01-01

75

Synthetic estrogen directly affects fish biomass and may indirectly disrupt aquatic food webs.  

PubMed

Endocrine-disrupting chemicals are known to alter the fitness of individual organisms via changes in growth, behavior, and reproduction. It is largely unknown, however, whether these effects cascade through the food web and indirectly affect other, less sensitive organisms. The authors present results from a mesocosm experiment whereby the effects of the synthetic estrogen 17?-ethinylestradiol (EE2) were quantified in pelagic communities. Treatment with EE2 at a concentration of 28?ng/L had no large effects on the pelagic communities composed only of phytoplankton and zooplankton. In communities where planktivorous roach (Rutilus rutilus) were also present, however, EE2 caused a significant reduction in fish biomass. Moreover, zooplankton biomass was higher in the EE2 treatments, suggesting that zooplankton may have been released from fish predation. Hence, the direct effect of EE2 on roach may have cascaded down the food web to produce positive indirect effects on zooplankton. This result was supported in complementary foraging experiments with roach, showing reduced foraging performance after exposure to EE2. Despite the observed negative effect of EE2 on roach and the positive indirect effect on zooplankton, these effects did not cascade to phytoplankton, possibly because only copepods, but not cladocerans-the major grazers in these systems-were released from fish predation. The authors conclude that the known reproductive impairment in fish by EE2 in combination with the disturbed foraging performance observed in the present study may be a disadvantage to fish that may result in increasing abundance or biomass of prey such as zooplankton. Hence, EE2 may have consequences for both the structure and function of freshwater communities. Environ Toxicol Chem 2014;33:930-936. © 2014 SETAC. PMID:24615795

Hallgren, Per; Nicolle, Alice; Hansson, Lars-Anders; Brönmark, Christer; Nikoleris, Lina; Hyder, Murtaza; Persson, Anders

2014-04-01

76

Host immunization shapes interspecific associations in trematode parasites.  

PubMed

1. Individuals of free-living organisms are commonly infected by multiple parasite species. Under such circumstances, positive or negative associations between the species are possible because of direct or indirect interactions, details in parasite transmission ecology and host-mediated factors. One possible mechanism underlying these processes is host immunity, but its role in shaping these associations has rarely been tackled experimentally. 2. In this study, we tested the effect of host immunization on associations between trematode parasites infecting eyes of fish. We first analysed the associations between three species (Diplostomum spathaceum, Diplostomum gasterostei and Tylodelphys clavata) in wild hosts, roach (Rutilus rutilus) and perch (Perca fluviatilis). Second, using rainbow trout (Oncorhynchus mykiss) as a model fish species, we experimentally investigated how sequential immunization of the host (i.e. one parasite species infects and immunizes the host first) could affect the associations between two of the species. 3. The results indicated that most of the associations were positive in wild hosts, which supports between-individual variation in host susceptibility, rather than competitive exclusion between the parasite species. However, positive associations were more common in roach than in perch, possibly reflecting differences in ecological conditions of exposure between the host species. The experimental data showed that positive associations between two of the species were eroded by host immunization against one of the parasite species. 4. We conclude that sequential immunization of hosts has a marked effect on interspecific parasite associations and basically can determine if positive associations are detected or not. This implies that correlative results suggesting non-interactive community structure in general may be obscured by the sequence of previous parasite exposure and corresponding dynamics of host immunization. PMID:19457020

Karvonen, Anssi; Seppälä, Otto; Tellervo Valtonen, E

2009-09-01

77

Patterns of brain morphology in mid-European Cyprinidae (Pisces, Teleostei): a quantitative histological study.  

PubMed

The present study considers patterns of brain morphology in 14 common species of mid-European Cyprinidae. Sixteen areas per brain were measured on serial cryostat sections by computer-aided planimetry. The volumes of these areas were expressed as % of the total brain volume. These brain centres (ranked according to falling values of the coefficient of variation VR, of the interspecific mean) are: Lobus facialis, L. vagus, central acustic area, Crista cerebellaris, Bulbus olfactorius, Eminentia granularis, Stratum opticum (of the optic tectum), Torus longitudinalis, Nucleus habenularis, Valvula cerebelli, Corpus cerebelli, Telencephalon, Tectum opticum, Diencephalon, Torus semicircularis, mesencephalic tegmentum. Seven primary sensory areas are leading in VR; highest in interspecific variability were the two special viscerosensory brainstem lobes for external (L. facialis) and internal (L. vagus) taste. Low in interspecific variability were integration centres (see above). By plotting the relative volumina of those brain centres which represent three major sensory modalities: brain stem chemosense, acoustico-lateralis and sense of vision, species are separated into three groups: 1) Most species scatter along an axis from moderately (Aspius aspius, Rutilus rutilus, Leuciscus cephalus, Scardinius erythrophthalmus, Alburnus alburnus, Chondrostoma nasus) to highly developed chemo- and acustico-lateralis centres (Vimba vimba, Abramis brama, Abramis ballerus). Blicca bjoercna is situated in the middle of this axis. Within the latter group the optic centres are evenly well developed. 2) Carassius carassius, Gobio gobio and Tinca tinca are characterized by relatively small acoustico-lateralis and optic areas, but highly developed chemocentres. 3) Pelecus cultratus is monotypic among the species investigated by having large acoustico-lateralis and optic, but modestly developed chemocentres. The brain patterns relative to life style are discussed. PMID:3418119

Kotrschal, K; Junger, H

1988-01-01

78

Precision of the age-length increments of three cyprinids: effects of fish number and sub-sampling strategy.  

PubMed

The effects of number of fish that are aged and scale sub-sampling strategies on the precision of estimates of mean age-length increments from populations of Rutilus rutilus, Leuciscus leuciscus and Leuciscus cephalus were tested. Analyses used data derived from river fish communities in eastern England, U.K.. Regarding the number of fishes analysed in each age group, for each species and mean fork-length increment at age, significant relationships were detected between sample size (n) and the coefficient of variation of the mean (Z) and mean length increment x? and measured variance (s(2)). This enabled calculation of the number of scales for producing a mean length increment at age according to n=ax?(b(-2))Zx?(-2). Outputs indicated that the number of scales requiring ageing increased substantially as precision increased, but with little variation between species per age category. Ageing between seven and 12 scales per age group would thus provide estimates at 10% precision. As the ages of fishes are not known in advance of scale ageing, the effect of scale sub-sampling regime on precision was also tested using randomized strategies of 10 fish per 5 mm, five per 5 mm, three per 5 mm, 10 per 10 mm, five per 10 mm and three per 10 mm. These were applied to the datasets and the consequences of their reduction in the number of scales for precision were determined using Z=a(0.5)x?((b/2)(-1))n(-0.5). When compared to no sub-sampling, three per 10 mm always significantly reduced data precision, whereas 10 per 5 mm never significantly reduced precision. These outputs can thus be applied to the design of fish sampling protocols where age and growth estimates are required, with the randomized sub-sampling likely to be the most useful strategy. PMID:24890409

Busst, G M; Britton, J R

2014-06-01

79

[Selective contrast enhancement of microscopical specimen by optical procedures for automatized quantitative image analysis (author's transl)].  

PubMed

One of the most important problems in automatic image analysis is the discrimination of features by, a certain range of grey levels. To obtain as many as possible different grey levels sufficient contrast is achieved mainly by specific staining. Alternative and supplementary methods are some micrscopical methods, not routinely used. Interference microscopy, interference reflexion microscopy and microfluorimetry are discussed in detail. These optical procedures enhance the contrast of specimen specifically without the necessity for the application of specimen specifically without the necessity for the application of sophisticated staining methods. In interference contrast, tissues can be separately detected by grey level discrimination due to varying concentration of dry mass; this is shown for a cornifying part of fish skin (breeding tubercle of Rutilus rutilus L.). Furthermore, very small amounts of dry matter can be determined with high precision, as demonstrated for a single tissue culture cell (XTH-cell). Automatisation of image analysis provides a unique opportunity for routine application of interferometric measurements. The principles of the procedures are outlined. By interference reflexion microscopy cellular attachment areas to a glass surface are visualized, providing a powerful tool in cellular diagnosis based on grey level discrimination (darkest parts correspond to zones of closest contact to the substratum). A fast migrating lymphocyte and stationary endothelial cells have been chosen for demonstration. Various histochemical problems can be solved elegantly by fluorescence methods, e.g. mitochondria in living cells are specifically stained by a fluorochrome (DASPMI) and the distribution of fluorescence intensity can be followed within the mitochondrial population of a cell. Fluorescence was recorded from fotographic negatives taken with a fluorescence microscope. Additionally a short comment is given on the application of polarisation microscopy for feature detection. PMID:392248

Bereiter-Hahn, J

1977-01-01

80

Oil shale processing as a source of aquatic pollution: monitoring of the biologic effects in caged and feral freshwater fish.  

PubMed

The biologic effects of the oil shale industry on caged rainbow trout (Oncorhynchus mykiss) as well as on feral perch (Perca fluviatilis) and roach (Rutilus rutilus) were studied in the River Narva in northeast Estonia. The River Narva passes the oil shale mining and processing area and thus receives elevated amounts of polycyclic aromatic hydrocarbons (PAHs), heavy metals, and sulfates. The effects of the chemical load were monitored by measuring cytochrome P4501A (CYP1A)-dependent monooxygenase (MO) activities [7-ethoxyresorufin O-deethylase and aryl hydrocarbon hydroxylase (AHH)] as well as conjugation enzyme activities [glutathione S-transferase (GST) and UDP-glucuronosyltransferase] in the liver of fish. CYP1A induction was further studied by detecting the amount and occurrence of the CYP1A protein. Histopathology of tissues (liver, kidney, spleen, and intestine) and the percentage of micronuclei in fish erythrocytes were also determined. Selected PAHs and heavy metals (Cd, Cu, Hg, and Pb) were measured from fish muscle and liver. In spite of the significant accumulation of PAHs, there was no induction of MO activities in any studied fish species. When compared to reference samples, AHH activities were even decreased in feral fish at some of the exposed sites. Detection of CYP1A protein content and the distribution of the CYP1A enzyme by immunohistochemistry also did not show extensive CYP1A induction. Instead, GST activities were significantly increased at exposed sites. Detection of histopathology did not reveal major changes in the morphology of tissues. The micronucleus test also did not show any evidence of genotoxicity. Thus, from the parameters studied, GST activity was most affected. The lack of catalytic CYP1A induction in spite of the heavy loading of PAHs was not studied but has been attributed to the elevated content of other compounds such as heavy metals, some of which can act as inhibitors for MOs. Another possible explanation of this lack of induction is that through adaptation processes the fish could have lost some of their sensitivity to PAHs. Either complex pollution caused by oil shale processing masked part of the harmful effects measured in this study, or oil shale industry did not have any severe effects on fish in the River Narva. Our study illustrates the difficulties in estimating risk in cases where there are numerous various contaminants affecting the biota. PMID:10464075

Tuvikene, A; Huuskonen, S; Koponen, K; Ritola, O; Mauer, U; Lindström-Seppä, P

1999-09-01

81

Oil shale processing as a source of aquatic pollution: monitoring of the biologic effects in caged and feral freshwater fish.  

PubMed Central

The biologic effects of the oil shale industry on caged rainbow trout (Oncorhynchus mykiss) as well as on feral perch (Perca fluviatilis) and roach (Rutilus rutilus) were studied in the River Narva in northeast Estonia. The River Narva passes the oil shale mining and processing area and thus receives elevated amounts of polycyclic aromatic hydrocarbons (PAHs), heavy metals, and sulfates. The effects of the chemical load were monitored by measuring cytochrome P4501A (CYP1A)-dependent monooxygenase (MO) activities [7-ethoxyresorufin O-deethylase and aryl hydrocarbon hydroxylase (AHH)] as well as conjugation enzyme activities [glutathione S-transferase (GST) and UDP-glucuronosyltransferase] in the liver of fish. CYP1A induction was further studied by detecting the amount and occurrence of the CYP1A protein. Histopathology of tissues (liver, kidney, spleen, and intestine) and the percentage of micronuclei in fish erythrocytes were also determined. Selected PAHs and heavy metals (Cd, Cu, Hg, and Pb) were measured from fish muscle and liver. In spite of the significant accumulation of PAHs, there was no induction of MO activities in any studied fish species. When compared to reference samples, AHH activities were even decreased in feral fish at some of the exposed sites. Detection of CYP1A protein content and the distribution of the CYP1A enzyme by immunohistochemistry also did not show extensive CYP1A induction. Instead, GST activities were significantly increased at exposed sites. Detection of histopathology did not reveal major changes in the morphology of tissues. The micronucleus test also did not show any evidence of genotoxicity. Thus, from the parameters studied, GST activity was most affected. The lack of catalytic CYP1A induction in spite of the heavy loading of PAHs was not studied but has been attributed to the elevated content of other compounds such as heavy metals, some of which can act as inhibitors for MOs. Another possible explanation of this lack of induction is that through adaptation processes the fish could have lost some of their sensitivity to PAHs. Either complex pollution caused by oil shale processing masked part of the harmful effects measured in this study, or oil shale industry did not have any severe effects on fish in the River Narva. Our study illustrates the difficulties in estimating risk in cases where there are numerous various contaminants affecting the biota. Images Figure 1 Figure 2

Tuvikene, A; Huuskonen, S; Koponen, K; Ritola, O; Mauer, U; Lindstrom-Seppa, P

1999-01-01

82

Bioaccumulation of mercury in muscle tissue of fish in the Elbe River (Czech Republic): multispecies monitoring study 1991-1996.  

PubMed

The study monitored mercury (Hg) contamination of fish muscle tissue at 13 geographical sites that can be regarded as crucial points for an ecotoxicological assessment of the Czech Republic section of the Elbe River. The descriptive part of the study was primarily aimed at comparative evaluation of the Hg load during the period 1991-1996. The conclusions were supported by multivariate statistical analyses of the content of Hg in the muscle tissue of 1251 fish belonging to 23 species with four dominant indicator species: Perca fluviatilis (n=163), Abramis brama (n=173), Rutilus rutilus (n=148), and Leuciscus cephalus (n=166). Considering data from 3- to 5-year-old fish, significantly increased contamination was detected in typical predators compared to the other fish species in all sites (P<0.001). On the other hand, omnivorous and planctivorous species were ranked as the least sensitive for Hg pollution. Perch appeared to be the most contaminated species in the sample with muscle Hg concentration in the range of 0.840-1.398 mg Hg kg(-1). Although less contaminated than perch, muscle contamination of bream sensitively separated differently contaminated sites; the highest load ranged from 0.368 to 0.543 mg Hg kg(-1). Time-related comparison of sampling campaigns revealed no significant trend changes, in either sediment samples or fish tissue. Thus, the analyses documented an evidently rather stabilized total Hg pollution in the Elbe River environment. Multivariate multispecies analyses found the age of analyzed individuals and the feeding strategy of a given species as the most important, however mutually interactive, covariates for Hg accumulation in muscle tissue. The analyses revealed decreasing sensitivity of older predator individuals to differentiate highly and moderately contaminated sites. Benthophagous species mostly kept their discrimination capacity toward contaminated sites in all age categories, with the exception of bream that was rather linked to the pattern typical for predator species. The unclear position of omnivorous species, represented namely by roach, corresponded with their weak bioindicator power, mainly in the young age categories. PMID:15883097

Dusek, L; Svobodová, Z; Janousková, D; Vykusová, B; Jarkovský, J; Smíd, R; Pavlis, P

2005-06-01

83

Isolation of estrogen receptor subtypes and vitellogenin genes: Expression in female Chalcalburnus tarichi.  

PubMed

Reproductively arrested gonadal development has been previously described in the teleost pearl mullet (Chalcalburnus tarichi, Cyprinidae) from Van Edremit Gulf of Lake Van, Turkey. Oocyte development in some females was arrested at the previtellogenic stage, while gonadosomatic index (GSI) and plasma 17?-estradiol (E2) level were low. A subset of the females was found to have normal ovaries and relatively higher plasma E2 and GSI. These two groups were termed reproductively arrested (RA) and reproductively non-arrested (RN) females. In this study, we cloned estrogen receptor (ER) isoforms (ER?, ER?1 and ER?2) and vitellogenin (Vtg), and their mRNA levels were measured in RA and RN fish tissues. C. tarichi ERs fell in the same clade with other fish ERs and ER? and ER?1 had 97% and 98% identity with the roach (Rutilus rutilus) ERs, respectively. Both Vtg and ER isoforms' mRNA abundance were higher in the liver than in the ovary and hypothalamus (liver>ovary>hypothalamus). The level of ER? mRNA was significantly lower in the liver, ovary and brain of RA fish than in the RN fish tissues. ER?1 mRNA levels were not different in the liver and ovary from RA and RN fish while ER?2 expression significantly increased in the liver and ovary from RA fish. All ER subtype expression was found to be lower in the brain from RA fish than RN fish. The level of Vtg mRNA was significantly lower in the liver and ovary from RA fish than RN fish tissue. These results suggest that ER subtypes are differentially regulated by E2, and their functions are also different in vitellogenesis. Analysis of organic contaminants in sediments revealed that C. tarichi living in Van Edremit Gulf of Lake Van are exposed to the contaminants bis(2-ethylhexyl) phthalate and 4,4(') DDT. We suggest that the RA fish represent a segment of the population that is more sensitive to exposure to endocrine disrupting compounds. PMID:24747933

Unal, Guler; Marquez, Emily C; Feld, Mara; Stavropoulos, Pericles; Callard, Ian P

2014-01-01

84

Evaluation of metal pollution in fish and water collected from Gorgan coast of the Caspian Sea, Iran.  

PubMed

In this study, concentrations of zinc, chromium, cadmium and lead were determined in water and three species of fish sampled from 10 selected stations along Gorgan coast using atomic absorption spectrophotometery, summer 2009. Mean lead levels in water and fish samples (119.50 ± 22.24 ?g/L and 113.80 ± 33.11 ?g/kg) were significantly higher than that of cadmium and chromium (p < 0.05). Cyprinus carpio had highest metals content than Mugila auratus and Rutilus frisikutum. The evaluated metals values in water collected from Gomishan wetland (Pb, Cd, Cr and Zn values are 145.31 ± 35.32, 120.46 ± 11.44, 96.47 ± 6.05 and 82.02 ± 34.37 ?g/L, respectively) were higher than the other sampling sites. The result is consistent with the findings accessed by evaluation of the metals in fish specimens. The metals concentrations in the fish and water samples were below the recommended limits. But, elevating levels of metals in water and fish made a serious concern about ecosystem and food chain contamination. PMID:22664555

Saeedi Saravi, S S; Karami, B; Karami, S; Shokrzadeh, M

2012-08-01

85

Vector and host relationships of California serogroup viruses in western Siberia.  

PubMed

During 1990 and 1991, adult mosquitoes were collected along the Ob River and its tributaries in western Siberia from approximately 51 degrees 18'N to 66 degrees 4'N. Fifteen virus strains were isolated from 74,196 mosquitoes tested in 1,874 pools. These included Tahyna virus from Aedes cataphylla-punctor subgroup (one) and Ae. excrucians (one), and Inkoo (INK) virus from Ae. communis (one), Ae. communis subgroup (one), Ae. hexodontus (two), Ae. punctor subgroup (two), Ae. punctor complex (one), and unidentified Aedes species (three). In addition, a single Ae. euedes yielded a strain of snowshoe hare (SSH) virus and a strain of Getah, an alphavirus. A Bunyamwera serogroup virus was isolated from Ae. excrucians. With the exception of the two isolates from a single mosquito, minimum infection rates among mosquito taxa ranged from 0.4 to 16.7 per 1,000. The INK virus isolates were widely distributed geographically; however, seven of the 10 isolates were from two sites north of the Arctic Circle. During 1991, sera from two mouse species, five vole species, and four shrew species were collected along the upper Ob River for serologic tests. The prevalence of neutralizing antibody to SSH virus in these sera was 80%. Prevalence rates in the four most abundant species were Apodemus agrarius, 73%; Clethrionomys rutilus, 71%; Microtus arvalis, 80%; and Sorex araneus, 91%. This is the first attempt to clarify the vector and vertebrate host relationships of California serogroup viruses in western Siberia. PMID:8352392

Mitchell, C J; Lvov, S D; Savage, H M; Calisher, C H; Smith, G C; Lvov, D K; Gubler, D J

1993-07-01

86

The trace element analysis in freshwater fish species, water and sediment in Iyidere stream (Rize-Turkey).  

PubMed

Many environmental problems like dam construction, agricultural debris, flooding and industrial establishments threaten Iyidere stream (Rize, Turkey) on the southeastern coast of the Black Sea (Turkey). The trace element concentrations in water, fish and sediments in lyidere stream (Rize, Turkey) were investigated in this study. The concentration of six different elements in ten freshwater fish species and sediment was determined using energy dispersive X-ray fluorescence method. A radioisotope excited X-ray fluorescence analysis using the method of multiple standard addition is applied for the elemental analysis of fish and sediments. Water samples for trace metals were analyzed using standard spectrophotometry methods. A qualitative analysis of spectral peaks showed that ten different freshwater fish samples (Chondrostoma colchicum, Chalcalburnus chalcoides, Salmo trutta labrax, Alburnoides bipunctatus, Leuciscus cephalus, Barbus taurus escherichia, Capoeta tinca, Neogobius kessleri, Rutilus frisii, Lampetra lanceolata) and sediment contained phosphorus (P), sulphur (S), chlorine (Cl), potassium (K), calcium (Ca) and titanium (Ti). Heavy metals as toxic elements for biota (Pb, Cd, Hg, Zn and Mn etc.) were not detected in fish, water and sediments. Thus, It can be declared that freshwater fish of Iyidere does not contains health risks for consumers in terms of metal pollution. PMID:24171246

Verep, Bulent; Mutlu, Cengiz; Apaydin, Gokhan; Cevik, Ugur

2012-07-15

87

Copper and zinc in four freshwater fish species from Lake Pamvotis (Greece).  

PubMed

Lake ecosystems are vulnerable to heavy-metal pollution. Fish samples are considered as one of the most indicative factors, in freshwater systems, for the estimation of trace metals pollution potential. Lake Pamvotis (NW Greece) is a typical Mediterranean ecosystem of great importance in regard to biodiversity and to aesthetic value. The fish species found most commonly in the lake are Cyprinus carpio, Silurus aristotelis, Rutilus ylikiensis, and Carassius gibelio. The aim of the present study is to evaluate the level of contamination of two essential heavy metals (copper and zinc) appearing at high concentrations in lake water in the above four fish species. Metal concentrations were measured by atomic absorption spectroscopy in three different tissues (muscle, liver, gonads) in order to assess the fish contamination. A two-factor analysis of variance, based on the procedure of general linear models, was employed in which fish species (four levels) and fish tissue (three levels) were examined for potential influence on Cu and Zn concentrations. Differences between level means per factor were treated using Tukey's multiple comparisons of means. The study showed that C. carpio and R. ylikiensis presented the highest metal content. Tissues analysis revealed that liver and gonads accumulated the highest levels of Cu and Zn. Metal concentration in the edible part of the examined fish (muscle) were in the safety-permissible levels for human consumption. PMID:14987866

Papagiannis, I; Kagalou, I; Leonardos, J; Petridis, D; Kalfakakou, V

2004-05-01

88

Attracted to the enemy: Aedes aegypti prefers oviposition sites with predator-killed conspecifics.  

PubMed

Oviposition habitat choices of species with aquatic larvae are expected to be influenced by both offspring risk of mortality due to predation, and offspring growth potential. Aquatic predators may indirectly influence growth potential for prey by reducing prey density and, for filter-feeding prey, by increasing bacterial food for prey via added organic matter (feces, partially eaten victims), creating the potential for interactive effects on oviposition choices. We tested the hypothesis that the mosquito Aedes aegypti preferentially oviposits in habitats with predatory Toxorhynchites larvae because of indirect effects of predation on chemical cues indicating bacterial abundance. We predicted that A. aegypti would avoid oviposition in sites with Toxorhynchites, but prefer to oviposit where bacterial food for larvae is abundant, and that predation by Toxorhynchites would increase bacterial abundances. Gravid A. aegypti were offered paired oviposition sites representing choices among: predator presence; the act of predation; conspecific density; dead conspecific larvae; and bacterial activity. A. aegypti preferentially oviposited in sites with Toxorhynchites theobaldi predation, and with killed conspecific larvae, but failed to detect preferences for other treatments. The antibiotic tetracycline eliminated the strongest oviposition preference. Both predation by Toxorhynchites and killed larvae increased bacterial abundances, suggesting that oviposition attraction is cued by bacteria. Our results show the potential for indirect effects, like trophic cascades, to influence oviposition choices and community composition in aquatic systems. Our results suggest that predators like Toxorhynchites may be doubly beneficial as biocontrol agents because of the attraction of ovipositing mosquitoes to bacterial by-products of Toxorhynchites feeding. PMID:24590205

Albeny-Simőes, Daniel; Murrell, Ebony G; Elliot, Simon L; Andrade, Mateus R; Lima, Eraldo; Juliano, Steven A; Vilela, Evaldo F

2014-06-01

89

Description of the pupa of Toxorhynch  

Microsoft Academic Search

The pupa of Toxorhynchites (Lynchie ZZa) rutii?us septentrionakk i s described and illustrated from a slide of a pupal skin presumably associated with the holotype male collected at Woodstock, Virginia. The adults of Toxorhynchites (LynchieZZal rutiZus septentriondis were described by Dyar 81 Knab (1906) (as MegarhCnus septent2?ionaZis) from a series of 13 d and 11 ? collected from various localities

Wallace A. Steffan; Neal L. Evenhuis

90

The type specimens of mosquitoes (Diptera, Culicidae) deposited in the entomological collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil  

Microsoft Academic Search

A list of type specimens of 100 mosquito species deposited in the Entomological collection of the Instituto Oswaldo Cruz is presented. It includes five holotypes belonging to the subfamily Anophelinae; 56 holotypes of Culicinae and two of Toxorhynchitinae. A lectotype is designated for Toxorhynchites fluminensis. The holotypes of six nominal species - Psorophora chiquitana, Psorophora circunflava, Psorophora melanota, Psorophora lanei,

Verônica Marchon-Silva; Ricardo Lourenço-de-Oliveira; Magaly Dolsan de Almeida; Adenildo da Silva-Vasconcelos; Jane Costa

1996-01-01

91

A Survey of the Mosquito Fauna in Palawan, Mindanao and North Luzon, Republic of the Philippines 1  

Microsoft Academic Search

Among the approximately 300 mosquito species known from the Philippines, 132 species belonging to 15 genera were identified from about 5,000 larval and adult specimens collected from the Philippines in 1981-82. Five species are reported from the Philippines for the first time: hmigeres pectinatus (Edwards), CuZex pez-@exus Leicester, Topomyia auriceps Brug, Toxorhynchites kempi (Edwards) and TX. Zeicesteri Theobald. In addition,

Ichiro Miyagi; Takako Toma; Masuhisa Tsukamoto; Motoyoshi Magi; Masahiro Horio; Benjamin D. Cabrera; Delfin G. Rivera

92

Mosquitoes of the mangrove forests of India: part four--Coringa, Andhra Pradesh.  

PubMed

Mosquitoes of 12 species belonging to 5 subgenera and 9 genera--Aedes, Anopheles, Culex, Diceromyia, Lorrainea, Rhinoskusea, Stegomyia, Toxorhynchites, and Verrallina--were recorded in the Coringa mangrove forest in Andhra Pradesh, India. Tree holes, crab holes, and swamp pools constituted the larval habitats with Lo. fumida being predominant in tree holes. Adults were found resting in tree holes, tree trunks, root bases, and in crab holes. Species involved in daytime feeding on humans were Ae. cancricomes, Cx. sitiens, Lo. fumida, Rh. wardi, and Ve. lugubris. PMID:17304921

Rajavel, A R; Natarajan, R; Vaidyanathan, K

2006-12-01

93

Mosquito fauna and breeding habitats of anophelines in Little Andaman Island, Andaman and Nicobar Islands, India.  

PubMed

A total of 37 species of mosquitoes belonging to nine genera--Anopheles, Aedes, Armigeres, Culex, Harpagomyia, Mansonia, Orthopodomyia, Toxorhynchites and Uranotaenia were collected from Little Andaman Island. Mosquitoes of nine Anopheles group of species--An. barbirostris, An. barbumbrosus, An. balabacensis, An. insulaeflorum, An. kochi, An. philippinensis, An. roperi, An. sundaicus and An. vagus were recorded. An. philippinensis was found to be the most predominant species. Species-specific breeding preference and association in various types of acquatic habitats have been reported. This is the first record of mosquito fauna of Little Andaman Island. PMID:14686116

Das, M K; Nagpal, B N; Ansari, M A

2002-01-01

94

Epidemiology of tree-hole breeding mosquitoes in the tropical rainforest of Imo State, south-east Nigeria.  

PubMed

The study of tree-hole breeding mosquitoes was carried out in the tropical rainforest of Imo State Nigeria (two rural areas and two forest reserves in some parts of Orlu Senatorial Zone) between May-October 2002. Using standard entomological procedures, two macrohabitats (natural tree-holes and bamboo traps) and two microhabitats (leaf axils of cocoyams/pineapples and leaf axils of plantain/banana) were sampled for various mosquito species. Mosquitoes were recovered from all the various biotypes sampled. Types of mosquitoes species encountered, their relative abundance, as well as genera varied significantly during the study (p<0.05). Four genera of mosquitoes: Aedes, Culex, Anopheles and Toxorhynchites were recovered while 16 species of mosquitoes encountered include: Aedes aegypti, Ae. africanus, Ae. simpsoni, Ae. albopictus, Ae. stokesi, Ae. taylori, Ae. apicoargenteus, Culex quinquefasciatus, Cx. nebulosus, Cx. trigripes, Cx. decens, Anopheles gambiae, An. funiestus, An. coustani and Toxorhynchites viridibasis. Most of the mosquitoes showed oviposition preferences for one or more habitats. The presence of Ae. africanus, Ae. simpsoni and Ae. aegypti indicate that the study areas were at risk of yellow fever epidemic. The presence of Anopheles and Culex species ensured endemicity of malaria and filariasis, while the recovery of Ae. albopictus in this region suggests a possible outbreak of dengue fever in future if not properly controlled. PMID:17655174

Anosike, Jude C; Nwoke, Bertram E B; Okere, Anthony N; Oku, Ene E; Asor, Joe E; Emmy-Egbe, Ifeyinwa O; Adimike, Desmond A

2007-01-01

95

Modelling the Active Hearing Process in Mosquitoes  

NASA Astrophysics Data System (ADS)

A simple microscopic mechanistic model is described of the active amplification within the Johnston's organ of the mosquito species Toxorhynchites brevipalpis. The model is based on the description of the antenna as a forced-damped oscillator coupled to a set of active threads (ensembles of scolopidia) that provide an impulsive force when they twitch. This twitching is in turn controlled by channels that are opened and closed if the antennal oscillation reaches a critical amplitude. The model matches both qualitatively and quantitatively with recent experiments. New results are presented using mathematical homogenization techniques to derive a mesoscopic model as a simple oscillator with nonlinear force and damping characteristics. It is shown how the results from this new model closely resemble those from the microscopic model as the number of threads approach physiologically correct values.

Avitabile, Daniele; Homer, Martin; Jackson, Joe; Robert, Daniel; Champneys, Alan

2011-11-01

96

Male and mosquito larvae survey at the Arenal-Tempisque irrigation project, Guanacaste, Costa Rica.  

PubMed

A monitoring of male and larvae of mosquitoes was conducted during 1991-1994, at the Irrigation Project in Arenal-Tempisque, Guanacaste, Costa Rica. CDC CO2-baited traps were used to collect adults of mosquitoes and dips were used for immatures of culicids. A total of 1,480 larvae and 1,129 males of culicids were identified resulting in, Aedes with 6 species, Anopheles, Mansonia and Psorophora with 2 species, Culex with 21 species and Haemagogus, Limatus, Toxorhynchites and Uranotaenia with only one species each. The results indicate that, as occurred in other countries, irrigation projects must be under strict monitoring programs to prevent and control possible health problems in which mosquitoes act as vectors. PMID:15162783

Vargas, Mario; Vargas, Jorge V

2003-01-01

97

Mosquitoes of the mangrove forests of India: Part 1--Bhitarkanika, Orissa.  

PubMed

In this first paper of a series on mosquitoes of the mangrove forests of India, details of mosquito species recorded in Bhitarkanika, Orissa, are presented. Forty-three species of mosquitoes belonging to 21 subgenera and 13 genera, Aedes, Anopheles, Armigeres, Coquillettidia, Culex, Ficalbia, Heizmannia, Lutzia, Mansonia, Ochlerotatus, Toxorhynchites, Uranotaenia, and Verrallina, were recorded. Predominant larval habitat was the tree holes, from which 15 species were taken. Adults were mostly found resting in crab holes, tree holes, and hoof prints in the forest area and on walls in the guesthouse area. About 14 species were caught in light traps, while 19 species landed on humans for feeding. Ae. franciscoi and Oc. feegradei are 2 new country records for India. Ae. cancricomes and Cx. perplexus, known only from Andaman Islands of India, are new records for mainland India. PMID:16033113

Rajavel, A R; Natarajan, R; Vaidyanathan, K

2005-06-01

98

Mosquitoes of the mangrove forests of India: part 5--Chorao, Goa, and Vikhroli, Maharashtra.  

PubMed

Mosquitoes of 14 species belonging to 9 subgenera and 9 genera were recorded in Chorao mangroves of Goa, and 12 species belonging to 9 subgenera and 7 genera in the mangroves of Vikhroli, Maharashtra, in India. Genera recorded were Aedes, Anopheles, Armigeres, Culex, Mansonia, Ochlerotatus, Orthopodomyia, Toxorhynchites, Uranotaenia, and Verrallina. Species common to both the mangroves were Ae. albopictus, Ae. novalbopictus, An. subpictus, Cx. sitiens, Oc. wardi, Ur. atra, and Ve. lugubris. Tree holes, crab holes, and swamp pools were the common larval habitats in Chorao, but tree holes were absent in Vikhroli. Adults of Ae. albopictus, Ae. novalbopictus, Ar. subalbatus, Cx. gelidus Cx. sitiens, Ma. indiana, Ur. atra, and Ve. lugubris were found landing on humans. Aedes novalbopictus, Oc. wardi, and Or. anopheloides are new records for the state of Goa. PMID:17847838

Rajavel, A R; Natarajan, R; Vaidyanathan, K; Soniya, V P

2007-06-01

99

Survey of new mosquito species of Meghalaya, India.  

PubMed

A study on mosquitoes in parts of Meghalaya revealed the presence of 64 species of mosquitoes under 10 genera. In monsoon (June to October) and post-monsoon (November to May) survey, 42 and 48 species of mosquitoes were detected, respectivily. Higher species diversity was found at lower elevations (up to 1050 m). Anopheles peditaeniatus, An. sinensis, Aedes vexans, Ae. nigrostriatus, Ae. annandalei, Culex. fuscocephala, Cx. pseudovishnui, Cx. murrelli, Cx. brevipalpis, Coquillettidia novochracea, Lutzia halifaxii, Malaya genurostris, Ml. jacobsoni, Tripteroides aranoides, Toxorhynchites minimus, Uranotaenia campestris, Ur. edwardsi and Ur. testaceae which were never reported earlier from Meghalaya state were detected in the present study and the findings were discussed with previously published reports from the state. PMID:24620578

Khan, S A; Dutta, P; Borah, J; Mahanta, J

2013-03-01

100

Mosquitoes of the mangrove forests of India: Part 3--Andaman and Nicobar Islands, including an update on the mosquito fauna of the islands.  

PubMed

ABSTRACT. Fifty-three mosquito species belonging to 20 subgenera and 18 genera--Aedes, Anopheles, Armigeres, Culex, Downsiomyia, Finlaya, Heizmannia, Kenknightia, Lorrainea, Lutzia, Ochlerotatus, Orthopodomyia, Rhinoskusea, Stegomyia, Toxorhynchites, Tripteroides, Uranotaenia, and Verrallina--were recorded in the mangroves of Andaman and Nicobar Islands, India, in collections made during May and June 2001. Larvae of 43 species were collected from different mangrove habitats. Together with collections made in nonmangrove areas, 83 species in 22 subgenera and 20 genera in total were recorded of which 33 species are new records for the Andaman and Nicobar Islands. Rhinoskusea wardi, Fl. flavipennis, and Ve. consonensis are 3 new country records for India. Collections included topotype specimens of St. seampi. Together with species known from earlier records, the mosquito fauna of Andaman and Nicobar Islands is updated to 107 species in 23 subgenera and 24 genera. PMID:17067033

Rajavel, A R; Natarajan, R

2006-09-01

101

Evaluation of Costa Rican copepods (Crustacea: Eudecapoda) for larval Aedes aegypti control with special reference to Mesocyclops thermocyclopoides.  

PubMed

This study attempted to find organisms for the biological control of the mosquito Aedes aegypti in Costa Rica. Copepods of the genera Arctodiaptomus, Eucylops, Mesocyclops, Megacyclops, and Thermocyclops were collected in several parts of the country and cultured for laboratory evaluations. Mesocyclops thermocyclopoides was the most successful species in reducing the number of larval Ae. aegypti (7.3 larvae in 24 h at a density of 200 Aedes/liter). Arctodiaptomus dorsalis, Eucyclops cf. bondi, Eucyclops leptacanthus, Megacyclops sp., and Thermocyclops decipens were not effective predators. In cage simulation trials, M. thermocyclopoides showed 100% larval reduction after 4 wk and adult mosquitoes disappeared after 7 wk. The copepod was able to survive in Aechmea sp. bromeliads under laboratory conditions. In field trials under 3 different climatic conditions M. thermocyclopoides survived 2-5 months in bromeliad leaf axils and 3-6 months in used car tires. In tires, this species reduced the number of larval Ae. aegypti 79, 90, and 99% in tropical dry, moderate, and humid climates, respectively. An El Nińo phenomenon affected the results by drought, which apparently also caused a decline in the population of the predatory mosquito Toxorhynchites haemorrhoidalis superbus. Considering these severe test conditions, M. thermocyclopoides might be a promising predator for mosquito control in Costa Rica. PMID:10612615

Schaper, S

1999-12-01

102

Entomological surveillance, spatial distribution, and diversity of Culicidae (Diptera) immatures in a rural area of the Atlantic Forest biome, State of Săo Paulo, Brazil.  

PubMed

Because of the high adaptive capacity of mosquitoes, studies that focus on transitional environments become very important, such as those in rural areas, which are considered as bridges between wild diseases and human populations of urban areas. In this study, a survey of the existing species of mosquitoes was performed in an Atlantic Forest area of the city of Santa Bárbara d'Oeste, Săo Paulo state, Brazil, using traps for immatures and analyzing the frequency and distribution of these insects over the sampling months. Five mosquito species were found: Aedes albopictus (the most frequent species), Aedes aegypti, Aedes fluviatilis, Culex quinquefasciatus, and Toxorhynchites theobaldi. The 4,524 eggs collected in ovitraps showed the presence of the tribe Aedini. Aedes aegypti and Ae. albopictus were identified after larval hatching in the laboratory, with different spatial distributions: the first of which coincides with the area of greatest diversity calculated using the Simpson index, while the second does not. The association of ecological analysis of spatial diversity with simple methods of data collection enables the identification of possible epidemiological risk situations and is a strategy that may be implemented to monitor ecological processes resulting from the interaction among different species of mosquitoes. PMID:24581361

Piovezan, Rafael; Rosa, Stéfany Larissa; Rocha, Matheus Luca; de Azevedo, Thiago Salomăo; Von Zuben, Cláudio José

2013-12-01

103

Classification of immature mosquito species according to characteristics of the larval habitat in the subtropical province of Chaco, Argentina.  

PubMed

To classify mosquito species based on common features of their habitats, samples were obtained fortnightly between June 2001-October 2003 in the subtropical province of Chaco, Argentina. Data on the type of larval habitat, nature of the habitat (artificial or natural), size, depth, location related to sunlight, distance to the neighbouring houses, type of substrate, organic material, vegetation and algae type and their presence were collected. Data on the permanence, temperature, pH, turbidity, colour, odour and movement of the larval habitat's water were also collected. From the cluster analysis, three groups of species associated by their degree of habitat similarity were obtained and are listed below. Group 1 consisted of Aedes aegypti. Group 2 consisted of Culex imitator, Culex davisi, Wyeomyia muehlensi and Toxorhynchites haemorrhoidalis separatus. Within group 3, two subgroups are distinguished: A (Psorophora ferox, Psorophora cyanescens, Psorophora varinervis, Psorophora confinnis, Psorophora cingulata, Ochlerotatus hastatus-oligopistus, Ochlerotatus serratus, Ochlerotatus scapularis, Culex intrincatus, Culex quinquefasciatus, Culex pilosus, Ochlerotatus albifasciatus, Culex bidens) and B (Culex maxi, Culex eduardoi, Culex chidesteri, Uranotaenia lowii, Uranotaenia pulcherrima, Anopheles neomaculipalpus, Anopheles triannulatus, Anopheles albitarsis, Uranotaenia apicalis, Mansonia humeralis and Aedeomyia squamipennis). Principal component analysis indicates that the size of the larval habitats and the presence of aquatic vegetation are the main characteristics that explain the variation among different species. In contrast, water permanence is second in importance. Water temperature, pH and the type of larval habitat are less important in explaining the clustering of species. PMID:21739026

Stein, Marina; Ludueńa-Almeida, Francisco; Willener, Juana Alicia; Almirón, Walter Ricardo

2011-06-01

104

Urban mosquito species (Diptera: Culicidae) of dengue endemic communities in the Greater Puntarenas area, Costa Rica  

PubMed Central

Field studies were conducted to determine the mosquito species richness in the urban area of Greater Puntarenas in Costa Rica. Two cross-sectional entomological surveys were performed in seven localities of Puntarenas: one survey was performed during the wet season and the other during the dry season. The sections evaluated were determined by applying a stratified cluster sampling method using satellite imagery, and a sample of 26 cells (100×100m) was selected for the study. The number of cells per locality was proportional to the area of each locality. The presence of mosquito larvae and pupae in water-filled artificial and natural containers was determined in each cell. Infestation was expressed as a diversity index per type of container (Ii). Eight types of larvae were identified (Aedes aegypti, Culex quinquefasciatus, Culex interrogator, Culex nigripalpus, Culex corniger, Culex tarsalis, Limatus durhamii and Toxorhynchites theobaldi) and in two cases it was only possible to identify the genus (Culex sp. and Uranotaenia sp.). A. aegypti was the most common species followed by C. quinquefascitus. Diversity of wet environments can explain the co-occurrence of various culicid species in some localities. Although A. aegypti is the only documented disease vector in the area, C quinquefasciatus, C. nigripalpus, and the other species of Culex could be considered potential vectors of other pathogens. The presence and ecology of all mosquito species should be studied to optimize surveillance and prevention of dengue and to prevent the emergence of other mosquito-transmitted diseases.

Troyo, Adriana; Solano, Mayra E.; Avendano, Adrian; Beier, John C.

2010-01-01

105

Review: artificial container-breeding mosquitoes and cemeteries: a perfect match.  

PubMed

Artificial container-breeding mosquitoes, such as Aedes aegypti, Ae. albopictus, and Culex pipiens, are well-recognized vectors of diseases throughout the world. Cemeteries are considered major sources of mosquitoes and the results of more than 30 studies concerning mosquitoes in cemeteries have been published over the last decade. The characteristics of these environments in regard to the availability of resources for mosquito development were discussed. Also, studies about early detection of Aedes vectors, ecological issues, and mosquito control performed in cemeteries were reviewed. Among 31 mosquito species found breeding in cemeteries from 16 countries, the invasive Ae. aegypti and Ae. albopictus were the most frequent ones. Species of the genus Ochlerotatus, Culex, Toxorhynchites, Culiseta, Armigeres, Lutzia, Uranotaenia, and Tripteroides were also reported. Overall, cemeteries are highly suitable habitats for artificial container-breeding mosquitoes due to the great availability of the different resources that they need (i.e. sugar substances, blood, shelter and water-filled containers). In addition, these places are mostly ideal settings to perform studies in urbanized areas because of high mosquito abundance, heterogeneity of macro- and microhabitats, and an easier access in comparison with private premises. However, the feasibility of a cemetery as a study area must be evaluated in each case considering the objectives of the study and cemetery characteristics. PMID:17300639

Vezzani, Darío

2007-02-01

106

Mosquito biodiversity of Dibru-Saikhowa biosphere reserve in Assam, India.  

PubMed

Entomological surveys were conducted for three consecutive years in core and buffer zone of the Dibru-Saikhowa biosphere reserve in pre monsoon, monsoon and post-monsoon seasons. A total of 52 species of mosquitoes under eleven genera have been detected. The genus Anopheles (18 species) was the predominant followed by Culex, Aedes, Mansonia, Armigeres, Mimomyia, Ochlerostatus, Malaya, Toxorhynchites, Ficalbia and Aedeomyia. The buffer zone of the forest reserve where human habitations are there exhibited the presence of maximum number of species (49 species under 10 genera) in comparison to core zone (42 species under 10 genera). In buffer zone, maximum numbers of species (38) were recorded in monsoon season followed by post- monsoon (35 species) and Pre-monsoon season (34 species). Whereas in core zone, maximum number of species were collected in post monsoon season followed by pre monsoon season and monsoon season. In Core and buffer zone, the maximum species were recorded from the ground pool habitat and slow flowing stream respectively. Among the disease vectors, the potential Japanese encephalitis vectors incriminated in India were very much prevalent. This study provides the list of available mosquito species recorded for the first time in the Dibru-Saikhowa biosphere reserve. PMID:21387924

Dutta, P; Prakash, A; Bhattacharyya, D R; Khan, S A; Gogoi, P R; Sharma, C K; Mahanta, J

2010-09-01

107

Mathematical modelling of the active hearing process in mosquitoes  

PubMed Central

Insects have evolved diverse and delicate morphological structures in order to capture the inherently low energy of a propagating sound wave. In mosquitoes, the capture of acoustic energy and its transduction into neuronal signals are assisted by the active mechanical participation of the scolopidia. We propose a simple microscopic mechanistic model of the active amplification in the mosquito species Toxorhynchites brevipalpis. The model is based on the description of the antenna as a forced-damped oscillator coupled to a set of active threads (ensembles of scolopidia) that provide an impulsive force when they twitch. This twitching is in turn controlled by channels that are opened and closed if the antennal oscillation reaches a critical amplitude. The model matches both qualitatively and quantitatively with recent experiments: spontaneous oscillations, nonlinear amplification, hysteresis, 2 : 1 resonances, frequency response and gain loss owing to hypoxia. The numerical simulations presented here also generate new hypotheses. In particular, the model seems to indicate that scolopidia located towards the tip of Johnston's organ are responsible for the entrainment of the other scolopidia and that they give the largest contribution to the mechanical amplification.

Avitabile, D.; Homer, M.; Champneys, A. R.; Jackson, J. C.; Robert, D.

2010-01-01

108

[Specific diversity and culicidian nuisance in the villages of N'gatty and Allaba in laguna area of Ivory Coast].  

PubMed

Entomological surveys were undertaken between June and December 2006 in N'gatty and Allaba. These villages are located in southern Ivory Coast in a laguna area in Dabou department. In these villages, there are large swampy areas, which have caused the multiplication of anthropophilic Culicidae. Mosquitoes have been collected at preimaginal stage at the time of the larval prospecting and at adult stage through human landing catch. Larval collections have been made using the classic method of "dipping". Larvae have been identified to the genus level. Then, they have been bred in the laboratory to identify adults. Adults collection has been made once a month during three consecutive nights by human landing catch inside houses. Adults have been identified to the specific level. Eight genera of mosquitoes have been collected in these two villages: Aedes, Anopheles, Coquillettidia, Culex, Eretmapodites, Mansonia, Toxorhynchites and Uranotaenia. Twenty-four species have been listed during this stu y. The genus Mansonia is the most predominant with 86% (N = 15,811) and 80% (N = 1,385), respectively, in N'gatty and Allaba. The average biting rate per day varies between N'gatty and Allaba. It is estimated to 308 bites per human per night (b/h/n) in N'gatty and 72 b/h/n in Allaba. In these villages, mosquito nuisance is mainly due to Mansonia with 264 b/h/n and 58 b/h/n, respectively, in N'gatty and Allaba. However, Anopheles gambiae s.l. average rate was 12 b/h/n in N'gatty and 2 b/h/n in Allaba. PMID:20632142

Fofana, D; Konan, K L; Djohan, V; Konan, Y L; Koné, A B; Doannio, J M C; N'goran, K E

2010-12-01

109

Mosquito larvicidal and antimicrobial activity of synthesized nano-crystalline silver particles using leaves and green berry extract of Solanum nigrum L. (Solanaceae: Solanales).  

PubMed

Silver nanoparticles (AgNPs) that are synthesized by using aqueous extracts of Solanum nigrum L., is a simple, non-toxic and ecofriendly green material. The present study is based on assessments of the larvicidal and antimicrobial activities of the synthesized AgNPs from fresh leaves, dry leaves and green berries of S. nigrum against larvae of Culex quinquefasciatus and Anopheles stephensi and four human pathogenic and five fish pathogenic bacteria respectively. The synthesized nanoparticles are characterized with UV-vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscope (TEM) analysis. The nanoparticles are spherical to polyhedral in shape with size of 50-100nm (average size of 56.6nm). In larvicidal bioassay with synthesized AgNPs, highest mortality are observed at 10ppm against An. stephensi with LC50 values of 1.33, 1.59, 1.56ppm and LC90 values of 3.97, 7.31, 4.76ppm for dry leaves, fresh leaves and berries respectively. Antibacterial activity test reveals better results against fish pathogenic bacteria than human pathogenic bacteria. Non target organism like Toxorhynchites larvae (mosquito predator), Diplonychus annulatum (predatory water-bug) and Chironomus circumdatus larvae (chironomid) are also exposed to respective lethal concentrations (to mosquito larvae) of dry nanoparticles and no abnormality in the non target organisms are recorded. These results suggest that the synthesized AgNPs of S. nigrum have the potential to be used as an ideal eco-friendly compound for the control of the mosquito larvae and harmful bacteria. PMID:24055718

Rawani, Anjali; Ghosh, Anupam; Chandra, Goutam

2013-12-01

110

Spinosad: a biorational mosquito larvicide for use in car tires in southern Mexico  

PubMed Central

Background Car tires are important habitats for mosquito development because of the high density populations they can harbor and their presence in urban settings. Water in experimental tires was treated with one of three insecticides or an untreated control. Aquatic invertebrates were sampled at weekly intervals. Eggs, larval and pupal samples were laboratory-reared to estimate seasonal fluctuations in Aedes aegypti and Ae. albopictus abundance. Results Spinosad treatments at 1 or 5?ppm (mg a.i./liter) provided 6–8?weeks of effective control of Ae. aegypti, Ae. albopictus, Culex quinquefasiatus and Cx. coronator larvae, both in the dry season and the rainy season when mosquito populations increased markedly in southern Mexico. Spinosad continued to provide partial control of larvae for several weeks after initial recolonization of treated tires. The larvicidal performance of VectoBac 12AS (Bacillus thuringiensis var. israelensis) was relatively poor with one week of complete control of Aedes spp. larvae and no discernible control of Culex spp., whereas the duration of larvicidal activity of 1% temephos mineral-based granules was intermediate between those of VectoBac and spinosad treatments. Populations of chironomids, ostracods and Toxorhynchites theobaldi were generally reduced in spinosad and temephos treatments, but were similar in control and VectoBac treatments. Conclusion The present study is the first to report spinosad as an effective larvicide against Cx. coronator, which is currently invading the southern United States. These results substantiate the use of spinosad as a highly effective mosquito larvicide, even in habitats such as unused car tires that can represent prolific sources of adult mosquitoes.

2012-01-01