Sample records for una planta procesadora

  1. Non-random sharing of Plantae genes.

    PubMed

    Chan, Cheong Xin; Bhattacharya, Debashish

    2011-05-01

    The power of eukaryote genomics relies strongly on taxon sampling. This point was underlined in a recent analysis of red algal genome evolution in which we tested the Plantae hypothesis that posits the monophyly of red, green (including plants) and glaucophyte algae. The inclusion of novel genome data from two mesophilic red algae enabled us to robustly demonstrate the sisterhood of red and green algae in the tree of life. Perhaps more exciting was the finding that >1,800 putative genes in the unicellular red alga Porphyridium cruentum showed evidence of gene-sharing with diverse lineages of eukaryotes and prokaryotes. Here we assessed the correlation between the putative functions of these shared genes and their susceptibility to transfer. It turns out that genes involved in complex interactive networks such as biological regulation and transcription/translation are less susceptible to endosymbiotic or horizontal gene transfer, when compared to genes with metabolic and transporter functions. PMID:21980581

  2. UNA PLANTA NUEVA DE LA SIERRA DE SEGURA (ESPAA), SUCCISELLA ANDREAE-MOUNAE, SP. NOV. (DIPSACACEAE)

    E-print Network

    Herrera, Carlos M.

    la Sierra de Segura (España), Succi- sella andreae-molinae, sp, nov. (Dipsacaceae). Anales Jard. Bot andreae-molinae, sp. nov. (Dipsacaceae). Anales Jard. Bot. Madrid 51(2): 249-254 (in Spa- nish). A new

  3. In planta functions of the Arabidopsis cytokinin receptor family

    Microsoft Academic Search

    Masayuki Higuchi; Melissa S. Pischke; Ari Pekka Mähönen; Kaori Miyawaki; Yukari Hashimoto; Motoaki Seki; Masatomo Kobayashi; Kazuo Shinozaki; Tomohiko Kato; Satoshi Tabata; Ykä Helariutta; Michael R. Sussman; Tatsuo Kakimoto

    2004-01-01

    Since their discovery as cell-division factors in plant tissue culture about five decades ago, cytokinins have been hypothesized to play a central role in the regulation of cell division and differentiation in plants. To test this hypothesis in planta, we isolated Arabidopsis plants lacking one, two, or three of the genes encoding a subfamily of histidine kinases (CRE1, AHK2, and

  4. Una poeta, una dramaturga y un director uruguayos

    E-print Network

    Bravo-Elizondo, Pedro

    1999-04-01

    , y en presencia de su madre. Esta mujer de noche se encerraba a escribir esa poesía erótica desenfrenada y la publica en vida, ¡imagínate vos! No fue una poesía que se conoció después de la muerte de la poeta. Una muchacha de la buena sociedad...

  5. Capacidad reproductiva de Pinus nigra subsp. salzmannii en relacin con la edad de la planta madre

    E-print Network

    Herrera, Carlos M.

    Capacidad reproductiva de Pinus nigra subsp. salzmannii en relación con la edad de la planta madre fertilidad del pino salgareño (Pinus nigra subsp. salzmannii) conforme los árboles envejecen. La fertilidad bosques de montaña del sur de Europa. Palabras clave: Pinus nigra, fertilidad, senescencia de las plantas

  6. Real-time PCR for the quantification of fungi in planta

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Methods enabling quantification of fungi in planta can be useful for a variety of applications. In combination with information on plant disease severity, indirect quantification of fungi in planta offers an additional tool in the screening of plants that are resistant to fungal diseases. In this ch...

  7. Bases morfofisiológicas para maior tolerância dos híbridos modernos de milho a altas densidades de plantas

    Microsoft Academic Search

    Luís Sangoi; Milton Luiz de Almeida; Paulo Regis Ferreira da Silva; Gilber Argenta

    2002-01-01

    RESUMO O lançamento de híbridos de milho tolerantes ao aumento da densidade de plantas contribuiu para o incremento do potencial produtivo da cultura na segunda metade do século XX. Objetiva-se com esta revisão de literatura discutir características morfológicas, fisiológicas, fenológicas e alométricas que contribuíram para maior adaptação do milho a elevadas densidades de plantas. Os processos de seleção utilizados pelos

  8. Una obra uruguaya en Madrid

    E-print Network

    Blaylock, Curtis

    1986-04-01

    SPRING 1986 103 Una obra uruguaya en Madrid Entre el 12 y el 15 de septiembre se ofrecieron al publico madrileño en el Centro Cultural de la Villa seis representaciones de Retrato de señora, con espejo (Vida y pasión de Margarita Xirgu). Este... sido una de las alumnas predilectas de Margarita Xirgu. Esta obra, previamente montada con éxito en Montevideo, Buenos Aires, Asunción y Barcelona, en Madrid ha sido un verdadero fracaso de taquilla, probablemente en gran medida porque ha coincidido...

  9. Collections and Exsiccates deposited in PRC herbarium Prague: Plantae Scandinaviae ex herb. G. V. Schotte

    E-print Network

    Svoboda, David

    Collections and Exsiccates deposited in PRC herbarium Prague: Plantae Scandinaviae ex herb. G. V Kosteletzky (incl. herb. Mann, Opiz p.p.) Flora exsiccata Austro-Hungarica Flora Hungarica exsiccata Caliciae

  10. Entropía de la información: una herramienta útil

    NASA Astrophysics Data System (ADS)

    Cincotta, P.

    En este trabajo se presenta una muy breve introducción al concepto de Entropía de la Información y se muestran distintas aplicaciones de una misma técnica para estudiar problemas tan variados como la determinación de periodicidad en una serie de tiempo arbitraria o de estimar el grado de caoticidad de una trayectoria en un sistema dinámico de N grados de libertad.

  11. Integracion de funciones de una variable Introduccion

    E-print Network

    Hurtado, Ana

    Tema 5 Integraci´on de funciones de una variable Introducci´on Este tema est´a dedicado al estudio distinta. 1. C´alculo de primitivas: si f : I R es una funci´on real de una variable definida sobre un intervalo abierto I, una primitiva de f es una funci´on derivable F : I R de forma que se cumple F (x) = f

  12. Una entrevista con Emilio Carballido.

    E-print Network

    Vé lez, Joseph F.

    1973-10-01

    FALL 1973 17 Una entrevista con Emilio Carballido JOSEPH F. VÉLEZ V. Señor Carballido, ¿podría decirme cuándo comenzó a interesarse en escribir? C. Fui un niño precoz que escribió mucho desde muy chamaquito. V. ¿Qué escribió primero: poesía... han presentado aquí? C. Todavía no. Pero parece que el teatro de Fuentes no es realista, y está más bien emparentado con otras corrientes. El teatro de Leñero tiende a ser teatro documental. El tuerto es rey, de Fuentes, tiene, parece...

  13. Una década de búsqueda del teatro hondureño

    E-print Network

    Reyes G., Candelario

    1992-04-01

    historia de los cuatrocientos años de Tegucigalpa (Alma Caballero). La década de los ochenta y la búsqueda de una plataforma de vanguardia El 2 de abril de 1982 surge la Comunidad Hondurena de Teatristas (COMHTE), una iniciativa particular de los grupos... de teatro popular e intento para generar una plataforma artística y de política cultural a tono con esa época de transformaciones sociales en Centroamérica. Su eslogan mismo, "Por SPRING 1992 109 un nuevo teatro para una nueva sociedad," fue punto...

  14. ARISTÓTELES Y UNA DISPUTA DE BIOÉTICA

    Microsoft Academic Search

    Alfonso Gómez-Lobo

    2006-01-01

    El objeto de este artículo es aplicar una estrategia desarrollada por Aristóteles al problema de determinar la naturaleza de un embrión durante sus primeros días. Aristóteles argumenta que lo decisivo es identificar aquello que hace que una porción de materia sea un ente de una cierta clase. Hoy sabemos que lo que hace que un conjunto de células sea un

  15. Cadeia alimentar "MODELO" montada pelo professor: Planta => Lagarta => Pssaro => Gato (Mimi) => Gato (Mimi) morto em

    E-print Network

    Morandini, Andre C.

    ) => Gato (Mimi) morto em decomposição. Figura 1: Foto do produtor utilizado no modelo do professor (planta feita com retalhos de tecido) Figura 2: Foto do consumidor primário usado no modelo do professor). #12;Figura 3: Foto do consumidor secundário usado no modelo do professor (pássaro feito som retalhos

  16. Planta (2007) 225:12211231 DOI 10.1007/s00425-006-0432-8

    E-print Network

    2007-01-01

    pollen development. Keywords MAP kinase kinase kinase · Anther · Pollen · Solanum chacoense GenbankPlanta (2007) 225:1221­1231 DOI 10.1007/s00425-006-0432-8 123 ORIGINAL PAPER The ScFRK2 MAP kinase kinase kinase from Solanum chacoense aVects pollen development and viability Martin O'Brien · Madoka Gray

  17. Phylogenetic Relationship of the Kingdoms Animalia, Plantae, and Fungi, Inferred from 23 Different Protein Species

    Microsoft Academic Search

    Naruo Nikoh; Nobuyoshi Hayase; Naoyuki Iwabe; Kei-ichi Kuma; Takashi Miyata

    The phylogenetic relationship among the kingdoms Animalia, Plantae, and Fungi remains uncertain, because of lack of solid fossil evidence. In spite of the extensive molecular phylogenetic analyses since the early report, this problem is a longstanding controversy; the proposed phylogenetic relationships differ for different authors, depending on the molecules and methods that they use. To settle this problem, we have

  18. Planta (1995)196:691-698 P l a n t ~ 9 Springer-Verlag 1995

    E-print Network

    Kronzucker, Herbert J.

    1995-01-01

    November 1994 Abstract. Using 13NH,~ as a tracer, compartmental ana- lyses for NH~- were performed in non - Compartmental analysis - Ni- trogen-13 - Picea Abbreviations and symbol: [NH+]cyt = cytoplasmic NH,~ concenPlanta (1995)196:691-698 P l a n t ~ 9 Springer-Verlag 1995 Compartmentation and flux

  19. Crassulacean Acid Metabolism: a Cause or Consequence of Oxidative Stress in Planta?

    Microsoft Academic Search

    Ewa Niewiadomska; Anne M. Borland

    The photosynthetic specialization of crassulacean acid metabolism (CAM) is typically found in plants growing in environments\\u000a where water and\\/or CO2 is limiting and, by analogy, where irradiance and daytime temperatures may be high. Such abiotic actors are known to lead\\u000a to the generation of reactive oxygen species (ROS) in planta which can elicit potentially damaging oxidative stress and\\/or\\u000a act as

  20. Development of an in planta method for transformation of alfalfa ( Medicago sativa )

    Microsoft Academic Search

    Jingsong Ye; Caius M. Rommens

    2008-01-01

    Conventional methods in transforming alfalfa (Medicago sativa) require multiple tissue culture manipulations that are time-consuming and expensive, while applicable only to a few highly\\u000a regenerable genotypes. Here, we describe a simple in planta method that makes it possible to transform a commercial variety without employing selectable marker genes. Basically, young\\u000a seedlings are cut at the apical node, cold-treated, and vigorously

  1. Rapid in planta evaluation of root expressed transgenes in chimeric soybean plants

    Microsoft Academic Search

    Jiarui Li; Timothy C. Todd; Harold N. Trick

    2010-01-01

    Production of stable transgenic plants is one factor that limits rapid evaluation of tissue specific transgene expression.\\u000a To hasten the assessment of transgenes in planta, we evaluated the use of chimeric soybean seedlings expressing transgenic\\u000a products in roots. Tap roots from four-day old seedlings (cultivars ‘Jack’ and KS4704) were excised and hairy roots were induced\\u000a from hypocotyls via Agrobacterium rhizogenes-mediated

  2. A quantitative PCR assay for accurate in planta quantification of the necrotrophic pathogen Phytophthora cinnamomi

    Microsoft Academic Search

    Leila Eshraghi; Nader Aryamanesh; Jonathan P. Anderson; Bryan Shearer; Jen A. McComb; Giles E. St. J. Hardy; Philip A. O’Brien

    A reliable method for measuring disease progression is important when evaluating susceptibility in host—pathogen interactions.\\u000a We describe a sensitive quantitative polymerase chain reaction (QPCR) assay that enables quantitative measurement of in planta DNA of the necrotrophic pathogen, Phytophthora cinnamomi, that avoids problems caused by variation in DNA extraction efficiency and degradation of host DNA during host tissue necrosis.\\u000a Normalization of

  3. UNA ENTIDAD EXTRAHOTELERA CUBANA EN MEJORA CONTINUA

    Microsoft Academic Search

    Lucía del Carmen Fajardo Villarroel; Beatriz del Carmen Duménigo Ruiz

    2011-01-01

    El estudio, enfoque a procesos y el correcto diseño de estos es de gran importancia en toda organización para la mejora continua y la toma de dediciones de la misma. Se tomó como objeto de estudio una entidad extrahotelera cubana necesitada de una correcta identificación, diseño e implantación de sus procesos. Para determinar e identificar los procesos que se ponen

  4. L'espai viscut: de una geografía sentida a una geografía reflexionada

    Microsoft Academic Search

    Xosé M. Souto González

    2007-01-01

    Las rutinas y hábitos que inundan una clase de geografía, los nuevos retos docentes en una sociedad saturada de información y con una disparidad de valores y actitudes ante la institución escolar, dificultan la actividad creativa e investigadora del profesorado. La rapidez con que transcurren los tiempos escolares y la inmediatez de las respuestas que exigen los alumnos hace difícil

  5. i Planta

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This application was designed for farmers, agriculture researchers, and gardeners. The application includes a calculator, a data logger, and a GPS mapping function. It was designed for vegetable farming but can be used for open field applications. The calculator solves for the amount of pesticide,...

  6. Highly Efficient Agrobacterium-Mediated Transformation of Wheat Via In Planta Inoculation

    NASA Astrophysics Data System (ADS)

    Risacher, Thierry; Craze, Melanie; Bowden, Sarah; Paul, Wyatt; Barsby, Tina

    This chapter details a reproducible method for the transformation of spring wheat using Agrobacterium tumefaciens via the direct inoculation of bacteria into immature seeds in planta as described in patent WO 00/63398(1. Transformation efficiencies from 1 to 30% have been obtained and average efficiencies of at least 5% are routinely achieved. Regenerated plants are phenotypically normal with 30-50% of transformation events carrying introduced genes at single insertion sites, a higher rate than is typically reported for transgenic plants produced using biolistic transformation methods.

  7. Comparative analysis of the in vitro and in planta secretomes from Mycosphaerella fijiensis isolates.

    PubMed

    Escobar-Tovar, Lina; Guzmán-Quesada, Mauricio; Sandoval-Fernández, Jorge A; Gómez-Lim, Miguel A

    2015-06-01

    Black Sigatoka, a devastating disease of bananas and plantains worldwide, is caused by the fungus Mycosphaerella fijiensis. Several banana cultivars such as 'Yangambi Km 5' and Calcutta IV, have been known to be resistant to the fungus, but the resistance has been broken in 'Yangambi Km 5' in Costa Rica. Since the resistance of this variety still persists in Mexico, the aim of this study was to compare the in vitro and in planta secretomes from two avirulent and virulent M. fijiensis isolates using proteomics and bioinformatics approaches. We aimed to identify differentially expressed proteins in fungal isolates that differ in pathogenicity and that might be responsible for breaking the resistance in 'Yangambi Km 5'. We were able to identify 90 protein spots in the secretomes of fungal isolates encoding 42 unique proteins and 35 differential spots between them. Proteins involved in carbohydrate transport and metabolism were more prevalent. Several proteases, pathogenicity-related, ROS detoxification and unknown proteins were also highly or specifically expressed by the virulent isolate in vitro or during in planta infection. An unknown protein representing a virulence factor candidate was also identified. These results demonstrated that the secretome reflects major differences between both M. fijiensis isolates. PMID:25986542

  8. Dynamic root exudation of sorgoleone and its in planta mechanism of action

    PubMed Central

    Dayan, Franck E.; Howell, J'Lynn; Weidenhamer, Jeffrey D.

    2009-01-01

    The oily droplets exuded from the root hairs of sorghum are composed of a 1:1 ratio of sorgoleone and its lipid resorcinol analogue. The production of these droplets appears to be suppressed when c. 20 ?g of exudate mg?1 root dry weight accumulates at the tip of the root hairs. However, more exudate is produced following gentle washing of the roots with water, suggesting that the biosynthesis of lipid benzoquinones and resorcinols is a dynamic process. Sorgoleone interferes with several molecular target sites, including photosynthetic electron transport, in in vitro assays. However, the in planta mechanism of action of sorgoleone remains controversial because it is not clear whether this lipid benzoquinone exuding from the roots of sorghum is taken up by roots of the receiving plants and translocated to their foliage where it must enter the chloroplast and inhibit PSII in the thylakoid membrane. Experiments designed to test the in planta mode of action of sorgoleone demonstrated that it has no effect on the photosynthesis of older plants, but inhibits photosynthesis in germinating seedlings. Sorgoleone is not translocated acropetally in older plants, but can be absorbed through the hypocotyl and cotyledonary tissues. Therefore, the mode of action of sorgoleone may be the result of inhibition of photosynthesis in young seedlings in concert with inhibition of its other molecular target sites in older plants. PMID:19357432

  9. Catástrofe, víctimas y trastornos: Hacia una definición en psicología

    Microsoft Academic Search

    Silvia Rosa; Sigales Ruiz

    Resumen: En este trabajo se lleva acabo una revisión de las nociones y conceptos que nos permitan definir desde la psicología a una catástrofe como un agente agresor causante de psico-traumatismos. Así mismo se iden- tifican los diversos niveles de implicación de una víctima, para luego hacer una breve revisión nosografía que nos permita distinguir las secuelas post- traumáticas resultadas

  10. Chromista is a major eukaryotic kingdom comprising algae and former protozoa that is evolutionarily entirely distinct from the kingdoms Plantae and Protozoa (Cavalier-Smith 2007). Chromist chloroplasts

    E-print Network

    Goldschmidt, Christina

    that is evolutionarily entirely distinct from the kingdoms Plantae and Protozoa (Cavalier-Smith 2007). Chromist chloroplasts were acquired secondarily by enslavement of a red alga, itself a member of kingdom Plantae 1 Chromista is a major eukaryotic kingdom comprising algae and former protozoa

  11. Evaluación asimétrica de una red neuronal artificial

    Microsoft Academic Search

    Maria Clara Aristizábal Restrepo

    2006-01-01

    Las redes neuronales artificiales (ANN)1 son modelos computacionales que tratan de replicar, de manera simplificada, el complejo funcionamiento del cerebro humano. Su capacidad de aprendizaje a través de ensayos repetidos, las ha hecho muy populares en una amplia variedad de aplicaciones en todas las ciencias. Su reciente implementación en economía se debe al hecho de que en las series económicas

  12. ¿Es la Ocratoxina A una micotoxina mutagénica?

    Microsoft Academic Search

    Arbillaga L; López de Cerain

    2004-01-01

    Resumen: La ocratoxina A es una micotoxina producida por especies de los géneros Aspergillus y Penicillium que a través de los alimentos puede pasar al ser humano. Su órgano diana es el riñón pero también es hepatotóxica, inmunotóxica y teratogéni- ca. Ha sido clasificada por la Agencia Internacional de Investigación contra el Cáncer (IARC) como posible carcinóge- no humano (clase

  13. In Planta Microsphere-Based Lateral Flow Leaf Biosensor in Maize.

    PubMed

    Wen, Jessica T; Castro, Carlos; Tsutsui, Hideaki

    2015-08-01

    Low-cost and quick detection of biotic stresses is critically important for protection of staple food crops such as maize in smallholder farms in developing countries, where access to improved seed varieties, fertilizers, and pesticides is limited due to financial and geographical reasons. Here, we report a new lateral flow detection technology directly integrated in a maize leaf, in which microspheres conjugated with analyte-specific capture antibodies are non-invasively injected. The antibody-conjugated microspheres capture and detect an analyte in a concentration-specific manner. In this study, we optimized microsphere size for effective infiltration and immobilization in the leaf, and further demonstrated detection of a fluorescent mock biomarker, fluorescein, in a live maize plant. This in planta lateral flow biosensor is the first of its kind and is expected to provide a low-cost and user-friendly detection method for biotic stresses in the field. PMID:25271045

  14. In planta transformation method for T-DNA transfer in orchids

    NASA Astrophysics Data System (ADS)

    Semiarti, Endang; Purwantoro, Aziz; Mercuriani, Ixora S.; Anggriasari, Anida M.; Jang, Seonghoe; Suhandono, Sony; Machida, Yasunori; Machida, Chiyoko

    2014-03-01

    Transgenic plant technology is an efficient tool to study the function of gene(s) in plant. The most popular and widely used technique is Agrobacterium-mediated transformation in which cocultivation was done by immersing the plant tissues/organ in overnight bacterial cultured for about 30 minutes to one hour under in vitro condition. In this experiment, we developed more easier technique that omitted the in vitro step during cocultivation with Agrobacterium, namely in planta transformation method. Pollinaria (compact pollen mass of orchid) of Phalaenopsis amabilis and Spathoglottis plicata orchids were used as target explants that were immersed into bacterial culture for 30 minutes, then dried up the pollinaria, the transformed pollinaria was used to pollinate orchid flowers. The T-DNA used for this experiments were Ubipro?PaFT/A. tumefaciens GV3101 for P. amabilis and MeEF1?2 pro?GUS/ A. tumefaciens LBA 4404 for S.plicata. Seeds that were produced from pollinated flowers were grown onto 10 mg/l hygromicin containing NP (New Phalaenopsis) medium. The existance of transgene in putative transformant protocorm (developing orchid embryo) genome was confirmed using PCR with specific primers of either PaFT or GUS genes. Histochemical GUS assay was also performed to the putative transformants. The result showed that transformation frequencies were 2.1 % in P. amabilis, and 0,53% in S. plicata. These results indicates that in planta transformation method could be used for Agrobacterium-mediated genetic transformation, with advantage easier and more secure work from contaminants than that of the in vitro method.

  15. Efficacy of phosphonic acid, metalaxyl-M and copper hydroxide against Phytophthora ramorum in vitro and in planta

    Microsoft Academic Search

    M. Garbelotto; T. Y. Harnik; D. J. Schmidt

    2008-01-01

    The ability of metalaxyl-M, phosphonic acid in the form of phosphonate, and copper hydroxide to inhibit different stages in the life cycle of Phytophthora ramorum , the causal agent of sudden oak death (SOD), was tested in vitro using 12 isolates from the North American forest lineage. In addition, experiments were conducted in planta to study the ability of phosphonic

  16. Single, Ancient Origin of a Plastid Metabolite Translocator Family in Plantae from an Endomembrane-Derived Ancestor

    Microsoft Academic Search

    Andreas P. M. Weber; Marc Linka; Debashish Bhattacharya

    2006-01-01

    The photosynthetic organelle (plastid) in red, green, and glaucophyte algae (Plantae) likely had a single origin from a cyanobacterial endosymbiosis (8, 17). This ancient (ca. 1 to 1.5 billion years ago) (10, 24) event was followed by the transfer of genetic material from the endosymbiont to the nuclear genome of the host, the evolution of a protein import apparatus for

  17. Abono para la planta medicinal Cobra fuerza en la Isla el estudio del valor teraputico de la flora tropical.

    E-print Network

    Quirk, Gregory J.

    que "muchas plantas americanas (como el tabaco, que se preparaba de diferentes maneras), eran de a otros colegas e investigadores de la Escuela de Farmacia. Entre ellos, figuran Ana Vélez, Ricardo pasos para que este logre la acreditación de la Asociación Americana de Museos. Jardín con historia A su

  18. Bacterial evolution by genomic island transfer occurs via DNA transformation in planta.

    PubMed

    Lovell, Helen C; Mansfield, John W; Godfrey, Scott A C; Jackson, Robert W; Hancock, John T; Arnold, Dawn L

    2009-09-29

    Our understanding of the evolution of microbial pathogens has been advanced by the discovery of "islands" of DNA that differ from core genomes and contain determinants of virulence. The acquisition of genomic islands (GIs) by horizontal gene transfer (HGT) is thought to have played a major role in microbial evolution. There are, however, few practical demonstrations of the acquisition of genes that control virulence, and, significantly, all have been achieved outside the animal or plant host. Loss of a GI from the bean pathogen Pseudomonas syringae pv. phaseolicola (Pph) is driven by exposure to the stress imposed by the plant's resistance response. Here, we show that the complete episomal island, which carries pathogenicity genes including the effector avrPphB, transfers between strains of Pph by transformation in planta and inserts at a specific att site in the genome of the recipient. Our results show that the evolution of bacterial pathogens by HGT may be achieved via transformation, the simplest mechanism of DNA exchange. This process is activated by exposure to plant defenses, when the pathogen is in greatest need of acquiring new genetic traits to alleviate the antimicrobial stress imposed by plant innate immunity. PMID:19747826

  19. Detection and Visualization of an Exopolysaccharide Produced by Xylella fastidiosa In Vitro and In Planta?

    PubMed Central

    Roper, M. Caroline; Greve, L. Carl; Labavitch, John M.; Kirkpatrick, Bruce C.

    2007-01-01

    Many phytopathogenic bacteria, such as Ralstonia solanacearum, Pantoea stewartii, and Xanthomonas campestris, produce exopolysaccharides (EPSs) that aid in virulence, colonization, and survival. EPS can also contribute to host xylem vessel blockage. The genome of Xylella fastidiosa, the causal agent of Pierce's disease (PD) of grapevine, contains an operon that is strikingly similar to the X. campestris gum operon, which is responsible for the production of xanthan gum. Based on this information, it has been hypothesized that X. fastidiosa is capable of producing an EPS similar in structure and composition to xanthan gum but lacking the terminal mannose residue. In this study, we raised polyclonal antibodies against a modified xanthan gum polymer similar to the predicted X. fastidiosa EPS polymer. We used enzyme-linked immunosorbent assay to quantify production of EPS from X. fastidiosa cells grown in vitro and immunolocalization microscopy to examine the distribution of X. fastidiosa EPS in biofilms formed in vitro and in planta and assessed the contribution of X. fastidiosa EPS to the vascular occlusions seen in PD-infected grapevines. PMID:17827325

  20. Detection and visualization of an exopolysaccharide produced by Xylella fastidiosa in vitro and in planta.

    PubMed

    Roper, M Caroline; Greve, L Carl; Labavitch, John M; Kirkpatrick, Bruce C

    2007-11-01

    Many phytopathogenic bacteria, such as Ralstonia solanacearum, Pantoea stewartii, and Xanthomonas campestris, produce exopolysaccharides (EPSs) that aid in virulence, colonization, and survival. EPS can also contribute to host xylem vessel blockage. The genome of Xylella fastidiosa, the causal agent of Pierce's disease (PD) of grapevine, contains an operon that is strikingly similar to the X. campestris gum operon, which is responsible for the production of xanthan gum. Based on this information, it has been hypothesized that X. fastidiosa is capable of producing an EPS similar in structure and composition to xanthan gum but lacking the terminal mannose residue. In this study, we raised polyclonal antibodies against a modified xanthan gum polymer similar to the predicted X. fastidiosa EPS polymer. We used enzyme-linked immunosorbent assay to quantify production of EPS from X. fastidiosa cells grown in vitro and immunolocalization microscopy to examine the distribution of X. fastidiosa EPS in biofilms formed in vitro and in planta and assessed the contribution of X. fastidiosa EPS to the vascular occlusions seen in PD-infected grapevines. PMID:17827325

  1. Advances in alfalfa mosaic virus-mediated expression of anthrax antigen in planta

    SciTech Connect

    Brodzik, R. [Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, PA 19107 (United States); Bandurska, K. [Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, PA 19107 (United States); Deka, D. [Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, PA 19107 (United States); Golovkin, M. [Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, PA 19107 (United States); Koprowski, H. [Biotechnology Foundation Laboratories, Thomas Jefferson University, Philadelphia, PA 19107 (United States)]. E-mail: h_koprowski@jefferson.edu

    2005-12-16

    Plant viruses show great potential for production of pharmaceuticals in plants. Such viruses can harbor a small antigenic peptide(s) as a part of their coat proteins (CP) and elicit an antigen-specific immune response. Here, we report the high yield and consistency in production of recombinant alfalfa mosaic virus (AlMV) particles for specific presentation of the small loop 15 amino acid epitope from domain-4 of the Bacillus anthracis protective antigen (PA-D4s). The epitope was inserted immediately after the first 25 N-terminal amino acids of AlMV CP to retain genome activation and binding of CP to viral RNAs. Recombinant AlMV particles were efficiently produced in tobacco, easily purified for immunological analysis, and exhibited extended stability and systemic proliferation in planta. Intraperitional injections of mice with recombinant plant virus particles harboring the PA-D4s epitope elicited a distinct immune response. Western blotting and ELISA analysis showed that sera from immunized mice recognized both native PA antigen and the AlMV CP.

  2. Root internalization, transport and in-planta survival of Salmonella enterica serovar Newport in sweet basil.

    PubMed

    Gorbatsevich, Elena; Sela Saldinger, Shlomo; Pinto, Riky; Bernstein, Nirit

    2013-02-01

    It is now acknowledged that food-borne pathogens present in the irrigation water or soil can become associated with crop plants in the field, penetrate internal plant tissues via the root, translocate and survive inside plants. Only little information is available concerning interaction between enteric pathogens and plants. The present study evaluated the potential for contamination of the aromatic plant, sweet basil during cultivation, by Salmonella enterica serovar Newport. Root internalization was plant-age-dependent, with the highest susceptibility occurring at the beginning of the rapid growth phase of the root. Higher incidence of internalization was detected in vegetative than reproductive plant organs, pointing at bacterial transport in the transpiration stream. Internalized Salmonella survived only planta survival was limited to less than 30?h. These findings imply that leaf surface contamination, rather than root internalization, may pose higher risk for human infection following consumption of contaminated basil. PMID:23757144

  3. VISITAS A LAS PÁGINAS DE UNA WEB DOCENTE UNIVERSITARIA

    Microsoft Academic Search

    Rafael Moreno; Rafael Martínez; Irene Martín

    2004-01-01

    El trabajo estudia las visitas recibidas durante un año y medio por las diferentes páginas de una web utilizada como herramienta complementaria para la docencia de una materia en una universidad presencial. Encuentra que dicho número es mayor, aunque de modo no significativo, en las páginas que contienen materiales para la teoría y prácticas de la mate- ria, y significativamente

  4. Capitulo 3 Integracion de funciones de una variable

    E-print Network

    Hurtado, Ana

    Cap´itulo 3 Integraci´on de funciones de una variable LA INTEGRAL INDEFINIDA. C ´ALCULO DE- jetivo es que dada una funci´on debemos encontrar otra cuya derivada coincida con la funci´on original. Comenzaremos dando algunas definiciones: Sea I un intervalo abierto de R. Dada una funci´on f : I R, se dice

  5. Una discusión en torno al concepto de bienestar

    Microsoft Academic Search

    Luis Fernando Gamboa; Darwin Cortés

    1999-01-01

    En este documento se quiere demostrar que una teoría que pretenda fundamentar la política social que no permita las comparaciones interpersonales es inadecuada. Por esta razón, el punto de partida es una crítica a la economía normativa neoclásica. Esta crítica consiste, en últimas, en una crítica al concepto de bienestar de los utilitaristas. Se plantea que el bienestar entendido como

  6. La dimension de los espacios osculadores a una variedad algebraica

    E-print Network

    Ruiz, Jesús M.

    La dimensi´on de los espacios osculadores a una variedad algebraica Raquel MALLAVIBARRENA dimensiones de los espacios osculadores a una variedad algebraica proyectiva y su relaci´on con la geometr, espacio osculador, superficies regladas, variedades de Veronese. 1. Introducci´on Sea X una variedad

  7. In planta side-chain glucosinolate modification in Arabidopsis by introduction of dioxygenase Brassica homolog BoGSL-ALK

    Microsoft Academic Search

    G. Li; C. F. Quiros

    2003-01-01

    Aliphatic glucosinolates and their derived isothiocyanates are important secondary metabolites in crucifers. Some of these compounds have beneficial activities such as carcinogen detoxification, pesticidal and antifungal properties, but others are anti-nutritional; the differences are largely due to side chain modifications. We report the cloning and in planta functionality analysis of BoGSL-ALK, a gene whose protein product influences side-chain modifications in

  8. Bioinspired engineering study of Plantae vascules for self-healing composite structures

    PubMed Central

    Trask, R. S.; Bond, I. P.

    2010-01-01

    This paper presents the first conceptual study into creating a Plantae-inspired vascular network within a fibre-reinforced polymer composite laminate, which provides an ongoing self-healing functionality without incurring a mass penalty. Through the application of a ‘lost-wax’ technique, orthogonal hollow vascules, inspired by the ‘ray cell’ structures found in ring porous hardwoods, were successfully introduced within a carbon fibre-reinforced epoxy polymer composite laminate. The influence on fibre architecture and mechanical behaviour of single vascules (located on the laminate centreline) when aligned parallel and transverse to the local host ply was characterized experimentally using a compression-after-impact test methodology. Ultrasonic C-scanning and high-resolution micro-CT X-ray was undertaken to identify the influence of and interaction between the internal vasculature and impact damage. The results clearly show that damage morphology is influenced by vascule orientation and that a 10 J low-velocity impact damage event is sufficient to breach the vasculature; a prerequisite for any subsequent self-healing function. The residual compressive strength after a 10 J impact was found to be dependent upon vascule orientation. In general, residual compressive strength decreased to 70 per cent of undamaged strength when vasculature was aligned parallel to the local host ply and a value of 63 per cent when aligned transverse. This bioinspired engineering study has illustrated the potential that a vasculature concept has to offer in terms of providing a self-healing function with minimum mass penalty, without initiating premature failure within a composite structure. PMID:19955122

  9. Abscisic acid metabolizing rhizobacteria decrease ABA concentrations in planta and alter plant growth.

    PubMed

    Belimov, Andrey A; Dodd, Ian C; Safronova, Vera I; Dumova, Valentina A; Shaposhnikov, Alexander I; Ladatko, Alexander G; Davies, William J

    2014-01-01

    Although endogenous phytohormones such as abscisic acid (ABA) regulate root growth, and many rhizobacteria can modulate root phytohormone status, hitherto there have been no reports of rhizobacteria mediating root ABA concentrations and growth by metabolising ABA. Using a selective ABA-supplemented medium, two bacterial strains were isolated from the rhizosphere of rice (Oryza sativa) seedlings grown in sod-podzolic soil and assigned to Rhodococcus sp. P1Y and Novosphingobium sp. P6W using partial 16S rRNA gene sequencing and phenotypic patterns by the GEN III MicroPlate test. Although strain P6W had more rapid growth in ABA-supplemented media than strain P1Y, both could utilize ABA as a sole carbon source in batch culture. When rice seeds were germinated on filter paper in association with bacteria, root ABA concentration was not affected, but shoot ABA concentration of inoculated plants decreased by 14% (strain P6W) and 22% (strain P1Y). When tomato (Solanum lycopersicum) genotypes differing in ABA biosynthesis (ABA deficient mutants flacca - flc, and notabilis - not and the wild-type cv. Ailsa Craig, WT) were grown in gnotobiotic cultures on nutrient solution agar, rhizobacterial inoculation decreased root and/or leaf ABA concentrations, depending on plant and bacteria genotypes. Strain P6W inhibited primary root elongation of all genotypes, but increased leaf biomass of WT plants. In WT plants treated with silver ions that inhibit ethylene perception, both ABA-metabolising strains significantly decreased root ABA concentration, and strain P6W decreased leaf ABA concentration. Since these changes in ABA status also occurred in plants that were not treated with silver, it suggests that ethylene was probably not involved in regulating bacteria-mediated changes in ABA concentration. Correlations between plant growth and ABA concentrations in planta suggest that ABA-metabolising rhizobacteria may stimulate growth via an ABA-dependent mechanism. PMID:24270514

  10. The In Planta Transcriptome of Ralstonia solanacearum: Conserved Physiological and Virulence Strategies during Bacterial Wilt of Tomato

    PubMed Central

    Jacobs, Jonathan M.; Babujee, Lavanya; Meng, Fanhong; Milling, Annett; Allen, Caitilyn

    2012-01-01

    ABSTRACT Plant xylem fluid is considered a nutrient-poor environment, but the bacterial wilt pathogen Ralstonia solanacearum is well adapted to it, growing to 108 to 109 CFU/g tomato stem. To better understand how R. solanacearum succeeds in this habitat, we analyzed the transcriptomes of two phylogenetically distinct R. solanacearum strains that both wilt tomato, strains UW551 (phylotype II) and GMI1000 (phylotype I). We profiled bacterial gene expression at ~6 × 108 CFU/ml in culture or in plant xylem during early tomato bacterial wilt pathogenesis. Despite phylogenetic differences, these two strains expressed their 3,477 common orthologous genes in generally similar patterns, with about 12% of their transcriptomes significantly altered in planta versus in rich medium. Several primary metabolic pathways were highly expressed during pathogenesis. These pathways included sucrose uptake and catabolism, and components of these pathways were encoded by genes in the scrABY cluster. A UW551 scrA mutant was significantly reduced in virulence on resistant and susceptible tomato as well as on potato and the epidemiologically important weed host Solanum dulcamara. Functional scrA contributed to pathogen competitive fitness during colonization of tomato xylem, which contained ~300 µM sucrose. scrA expression was induced by sucrose, but to a much greater degree by growth in planta. Unexpectedly, 45% of the genes directly regulated by HrpB, the transcriptional activator of the type 3 secretion system (T3SS), were upregulated in planta at high cell densities. This result modifies a regulatory model based on bacterial behavior in culture, where this key virulence factor is repressed at high cell densities. The active transcription of these genes in wilting plants suggests that T3SS has a biological role throughout the disease cycle. PMID:22807564

  11. Una spiegazione della fretta e della infelicità contemporanee

    Microsoft Academic Search

    Stefano Bartolini

    La prima tesi di questo lavoro è che esiste una abbondante evidenza empirica che dimostra che la crescita economica ha tradito le sue promesse di aumento del tempo libero e della felicità. La seconda tesi è che la teoria economica mainstream è del tutto impotente a spiegare tale tradimento. D'altra parte una spiegazione congiunta del tradimento di tali promesse sembra

  12. Consideraciones para una política económica regional en Colombia

    Microsoft Academic Search

    Juan David Barón; Gerson Javier Pérez; Peter Rowland

    2004-01-01

    Este trabajo propone un marco de trabajo para una política económica regional en Colombia. Se estudian las características regionales y se muestra que las disparidades regionales son significativas y persistentes en el tiempo. Por ello, se hace necesario llevar a cabo una política económica que promueva el desarrollo de las regiones más pobres de país. Este estudio aprovecha lecciones de

  13. Una introduccion al Analisis Formal de Conceptos en PVS

    E-print Network

    Alonso, José A.

    Una introducci´on al An´alisis Formal de Conceptos en PVS Jos´e A. Alonso, J. Borrego, M.J. Hidalgo una formalizaci´on en PVS del An´alisis Formal de Conceptos. Inicialmente, formalizamos la noci´on de concepto y establecemos que el conjunto de conceptos de un contexto formal tiene estructura de ret

  14. Una búsqueda de la identidad regional em Astrometria

    NASA Astrophysics Data System (ADS)

    Abad, C.

    2003-11-01

    La idea sobre una reunión latino-americana que congregara a las personas que desarrollan su trabajo en el área de la astrometría, se gestó a partir de una de las reuniones que el proyecto QUEST realizaba anualmente en Mérida (Venezuela). El proyecto QUEST (Quasars Equatorial Survey Team) celebraba su IV Reunión Anual, en la cual se hacía una revisón de la marcha del proyecto durante el último año. Además, se exponían los logros científicos y técnicos alcanzados hasta el momento y se planificaba el futuro del mismo para el período anual siguiente.

  15. Controle de qualidade de produtos à base de plantas medicinais comercializados na cidade do Recife-PE: erva-doce (Pimpinella anisum L.), quebra-pedra (Phyllanthus spp.), espinheira santa (Maytenus ilicifolia Mart.) e camomila (Matricaria recutita L.) 1

    Microsoft Academic Search

    V. T. Nascimento; E. U. Lacerda; J. G. Melo; E. L. C. Amorim

    RESUMO: As plantas têm sido bastante utilizadas para a cura de doenças e por isso, a cada dia, mais fitoterápicos têm sido colocados à disposição no mercado. Apesar do aumento no consumo de diferentes produtos a base de plantas medicinais, fatores como a precariedade na fiscalização da produção e da venda têm comprometido a qualidade dos mesmos. O presente trabalho

  16. Manejando Bicicleta de una Manera Segura a Westlake

    E-print Network

    Karplus, Kevin

    reprogramado para que los ciclistas tengan suficiente tiempo para cru- zar la calle. Las colinas tan empinadas calles pero deberían caminar con sus bicicletas una cuadra y cruzar en High. El viaje de regreso es más

  17. Phytohormonal networks promote differentiation of fiber initials on pre-anthesis cotton ovules grown in vitro and in planta.

    PubMed

    Kim, Hee Jin; Hinchliffe, Doug J; Triplett, Barbara A; Chen, Z Jeffrey; Stelly, David M; Yeater, Kathleen M; Moon, Hong S; Gilbert, Matthew K; Thyssen, Gregory N; Turley, Rickie B; Fang, David D

    2015-01-01

    The number of cotton (Gossypium sp.) ovule epidermal cells differentiating into fiber initials is an important factor affecting cotton yield and fiber quality. Despite extensive efforts in determining the molecular mechanisms regulating fiber initial differentiation, only a few genes responsible for fiber initial differentiation have been discovered. To identify putative genes directly involved in the fiber initiation process, we used a cotton ovule culture technique that controls the timing of fiber initial differentiation by exogenous phytohormone application in combination with comparative expression analyses between wild type and three fiberless mutants. The addition of exogenous auxin and gibberellins to pre-anthesis wild type ovules that did not have visible fiber initials increased the expression of genes affecting auxin, ethylene, ABA and jasmonic acid signaling pathways within 1 h after treatment. Most transcripts expressed differentially by the phytohormone treatment in vitro were also differentially expressed in the ovules of wild type and fiberless mutants that were grown in planta. In addition to MYB25-like, a gene that was previously shown to be associated with the differentiation of fiber initials, several other differentially expressed genes, including auxin/indole-3-acetic acid (AUX/IAA) involved in auxin signaling, ACC oxidase involved in ethylene biosynthesis, and abscisic acid (ABA) 8'-hydroxylase an enzyme that controls the rate of ABA catabolism, were co-regulated in the pre-anthesis ovules of both wild type and fiberless mutants. These results support the hypothesis that phytohormonal signaling networks regulate the temporal expression of genes responsible for differentiation of cotton fiber initials in vitro and in planta. PMID:25927364

  18. Expandiendo los límites del teatro: Una entrevista con Astrid Hadad

    E-print Network

    Alzate, Gastó n

    1997-04-01

    SPRING 1997 153 Expandiendo los límites del teatro: Una entrevista con Astrid Hadad Gastón Álzate Astrid Hadad, nacida en Chetumal, Quintana Roo (México) y de padres libaneses, es hoy en día una de las artistas más significativas dentro de un... consolidado un espacio propio. Esto se puede apreciar en el trabajo desarrollado por Jesusa Rodríguez, Liliana Felipe y la misma Astrid Hadad. Es importante anotar que existe un buen número de artistas que ha venido desarrollando trabajos paralelos, como es...

  19. El Text audiovisual: anàlisi des d'una perspectiva mediològica

    Microsoft Academic Search

    Anna Tous i Rovirosa

    2009-01-01

    El text audiovisual: Anàlisi des d'una perspectiva mediològica és una investigació\\u000ade cinc sèries dels Estats Units: El ala oeste de la Casa Blanca (The\\u000awest wing, NBC: 1999-2006), Perdidos (Lost, ABC: 2004- ), Mujeres desesperadas\\u000a(Desperate housewives, ABC: 2004- ), House (House, Fox: 2004- ) i\\u000aCSI: Las Vegas (CSI: Crime Scene Investigation, CBS: 2000- ), pel que fa

  20. Geoffrey West, James H. Brown y Brian Enquist desarrollaron un modelo fractal de la ramificacin de sistemas de distribucin en plantas y animales

    E-print Network

    Nacional Autónoma de México, Universidad

    Geoffrey West, James H. Brown y Brian Enquist desarrollaron un modelo fractal de la ramificación de sistemas de distribución en plantas y animales que podría explicar el misterio del escalamiento de la tasa metabólica. Como todas las ideas nuevas, el modelo ha recibido tanto aplausos como agrias críticas. Veamos

  1. The in planta transcriptome of Ralstonia solanacearum: conserved physiological and virulence strategies during bacterial wilt of tomato.

    PubMed

    Jacobs, Jonathan M; Babujee, Lavanya; Meng, Fanhong; Milling, Annett; Allen, Caitilyn

    2012-01-01

    Plant xylem fluid is considered a nutrient-poor environment, but the bacterial wilt pathogen Ralstonia solanacearum is well adapted to it, growing to 10(8) to 10(9) CFU/g tomato stem. To better understand how R. solanacearum succeeds in this habitat, we analyzed the transcriptomes of two phylogenetically distinct R. solanacearum strains that both wilt tomato, strains UW551 (phylotype II) and GMI1000 (phylotype I). We profiled bacterial gene expression at ~6 × 10(8) CFU/ml in culture or in plant xylem during early tomato bacterial wilt pathogenesis. Despite phylogenetic differences, these two strains expressed their 3,477 common orthologous genes in generally similar patterns, with about 12% of their transcriptomes significantly altered in planta versus in rich medium. Several primary metabolic pathways were highly expressed during pathogenesis. These pathways included sucrose uptake and catabolism, and components of these pathways were encoded by genes in the scrABY cluster. A UW551 scrA mutant was significantly reduced in virulence on resistant and susceptible tomato as well as on potato and the epidemiologically important weed host Solanum dulcamara. Functional scrA contributed to pathogen competitive fitness during colonization of tomato xylem, which contained ~300 µM sucrose. scrA expression was induced by sucrose, but to a much greater degree by growth in planta. Unexpectedly, 45% of the genes directly regulated by HrpB, the transcriptional activator of the type 3 secretion system (T3SS), were upregulated in planta at high cell densities. This result modifies a regulatory model based on bacterial behavior in culture, where this key virulence factor is repressed at high cell densities. The active transcription of these genes in wilting plants suggests that T3SS has a biological role throughout the disease cycle. IMPORTANCE Ralstonia solanacearum is a widespread plant pathogen that causes bacterial wilt disease. It inflicts serious crop losses on tropical farmers, with major economic and human consequences. It is also a model for the many destructive microbes that colonize the water-conducting plant xylem tissue, which is low in nutrients and oxygen. We extracted bacteria from infected tomato plants and globally identified the biological functions that R. solanacearum expresses during plant pathogenesis. This revealed the unexpected presence of sucrose in tomato xylem fluid and the pathogen's dependence on host sucrose for virulence on tomato, potato, and the common weed bittersweet nightshade. Further, R. solanacearum was highly responsive to the plant environment, expressing several metabolic and virulence functions quite differently in the plant than in pure culture. These results reinforce the utility of studying pathogens in interaction with hosts and suggest that selecting for reduced sucrose levels could generate wilt-resistant crops. PMID:22807564

  2. Diseo y desarrollo del subsistema de deteccin y reaccin de una plataforma para la prevencin de

    E-print Network

    Garcia-Alfaro, Joaquin

    Diseño y desarrollo del subsistema de detección y reacción de una plataforma para la prevención de artículo presentamos una plataforma que proporciona una solución descentralizada para prevenir este tipo de presentamos el diseño y desarrollo del subsistema de detección y reacción de una plataforma que proporciona

  3. A toolset of aequorin expression vectors for in planta studies of subcellular calcium concentrations in Arabidopsis thaliana

    PubMed Central

    Mehlmer, Norbert; Parvin, Nargis; Hurst, Charlotte H.; Knight, Marc R.; Teige, Markus; Vothknecht, Ute C.

    2014-01-01

    Calcium has long been acknowledged as one of the most important signalling components in plants. Many abiotic and biotic stimuli are transduced into a cellular response by temporal and spatial changes in cellular calcium concentration and the calcium-sensitive protein aequorin has been exploited as a genetically encoded calcium indicator for the measurement of calcium in planta. The objective of this work was to generate a compatible set of aequorin expression plasmids for the generation of transgenic plant lines to measure changes in calcium levels in different cellular subcompartments. Aequorin was fused to different targeting peptides or organellar proteins as a means to localize it to the cytosol, the nucleus, the plasma membrane, and the mitochondria. Furthermore, constructs were designed to localize aequorin in the stroma as well as the inner and outer surface of the chloroplast envelope membranes. The modular set-up of the plasmids also allows the easy replacement of targeting sequences to include other compartments. An additional YFP-fusion was included to verify the correct subcellular localization of all constructs by laser scanning confocal microscopy. For each construct, pBin19-based binary expression vectors driven by the 35S or UBI10 promoter were made for Agrobacterium-mediated transformation. Stable Arabidopsis lines were generated and initial tests of several lines confirmed their feasibility to measure calcium signals in vivo. PMID:22213817

  4. Functional characterisation of metal(loid) processes in planta through the integration of synchrotron techniques and plant molecular biology

    PubMed Central

    Donner, Erica; Punshon, Tracy; Guerinot, Mary Lou; Lombi, Enzo

    2013-01-01

    Functional characterisation of the genes regulating metal(loid) homeostasis in plants is a major focus of crop biofortification, phytoremediation, and food security research. This paper focuses on the potential for advancing plant metal(loid) research by combining molecular biology and synchrotron-based techniques. Recent advances in x-ray focussing optics and fluorescence detection have greatly improved the potential of synchrotron techniques for plant science research, allowing metal(loids) to be imaged in vivo in hydrated plant tissues at sub-micron resolution. Laterally resolved metal(loid) speciation can also be determined. By using molecular techniques to probe the location of gene expression and protein localisation and combining it with this synchrotron-derived data, functional information can be effectively and efficiently assigned to specific genes. This paper provides a review of the state of the art in this field, and provides examples as to how synchrotron-based methods can be combined with molecular techniques to facilitate functional characterisation of genes in planta. PMID:22200921

  5. Agrobacterium tumefaciens Deploys a Superfamily of Type VI Secretion DNase Effectors as Weapons for Interbacterial Competition In Planta

    PubMed Central

    Ma, Lay-Sun; Hachani, Abderrahman; Lin, Jer-Sheng; Filloux, Alain; Lai, Erh-Min

    2014-01-01

    Summary The type VI secretion system (T6SS) is a widespread molecular weapon deployed by many Proteobacteria to target effectors/toxins into both eukaryotic and prokaryotic cells. We report that Agrobacterium tumefaciens, a soil bacterium that triggers tumorigenesis in plants, produces a family of type VI DNase effectors (Tde) that are distinct from previously known polymorphic toxins and nucleases. Tde exhibits an antibacterial DNase activity that relies on a conserved HxxD motif and can be counteracted by a cognate immunity protein, Tdi. In vitro, A. tumefaciens T6SS could kill Escherichia coli but triggered a lethal counterattack by Pseudomonas aeruginosa upon injection of the Tde toxins. However, in an in planta coinfection assay, A. tumefaciens used Tde effectors to attack both siblings cells and P. aeruginosa to ultimately gain a competitive advantage. Such acquired T6SS-dependent fitness in vivo and conservation of Tde-Tdi couples in bacteria highlights a widespread antibacterial weapon beneficial for niche colonization. PMID:24981331

  6. Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation

    PubMed Central

    Maloney, Victoria J.; Park, Ji-Young; Unda, Faride; Mansfield, Shawn D.

    2015-01-01

    Bioinformatic analysis indicates that sucrose phosphate synthase (SPS) contains a putative C-terminal sucrose phosphate phosphatase (SPP)-like domain that may facilitates the binding of SPP. If an SPS–SPP enzyme complex exists, it may provide sucrose biosynthesis with an additional level of regulation, forming a direct metabolic channel for sucrose-6-phosphate between these two enzymes. Herein, the formation of an enzyme complex between SPS and SPP was examined, and the results from yeast two-hybrid experiments suggest that there is indeed an association between these proteins. In addition, in planta bioluminescence resonance energy transfer (BRET) was observed in Arabidopsis seedlings, providing physical evidence for a protein interaction in live cells and in real time. Finally, bimolecular fluorescence complementation (BiFC) was employed in an attempt to detect SPS–SPP interactions visually. The findings clearly demonstrated that SPS interacts with SPP and that this interaction impacts soluble carbohydrate pools and affects carbon partitioning to starch. Moreover, a fusion construct between the two genes promotes plant growth in both transgenic Arabidopsis and hybrid poplar. PMID:25873678

  7. Sucrose phosphate synthase and sucrose phosphate phosphatase interact in planta and promote plant growth and biomass accumulation.

    PubMed

    Maloney, Victoria J; Park, Ji-Young; Unda, Faride; Mansfield, Shawn D

    2015-07-01

    Bioinformatic analysis indicates that sucrose phosphate synthase (SPS) contains a putative C-terminal sucrose phosphate phosphatase (SPP)-like domain that may facilitates the binding of SPP. If an SPS-SPP enzyme complex exists, it may provide sucrose biosynthesis with an additional level of regulation, forming a direct metabolic channel for sucrose-6-phosphate between these two enzymes. Herein, the formation of an enzyme complex between SPS and SPP was examined, and the results from yeast two-hybrid experiments suggest that there is indeed an association between these proteins. In addition, in planta bioluminescence resonance energy transfer (BRET) was observed in Arabidopsis seedlings, providing physical evidence for a protein interaction in live cells and in real time. Finally, bimolecular fluorescence complementation (BiFC) was employed in an attempt to detect SPS-SPP interactions visually. The findings clearly demonstrated that SPS interacts with SPP and that this interaction impacts soluble carbohydrate pools and affects carbon partitioning to starch. Moreover, a fusion construct between the two genes promotes plant growth in both transgenic Arabidopsis and hybrid poplar. PMID:25873678

  8. The maternal chromosome set is the target of the T-DNA in the in planta transformation of Arabidopsis thaliana.

    PubMed Central

    Bechtold, N; Jaudeau, B; Jolivet, S; Maba, B; Vezon, D; Voisin, R; Pelletier, G

    2000-01-01

    In planta transformation methods are now commonly used to transform Arabidopsis thaliana by Agrobacterium tumefaciens. The origin of transformants obtained by these methods has been studied by inoculating different floral stages and examining gametophytic expression of an introduced beta-glucuronidase marker gene encoding GUS. We observed that transformation can still occur after treating flowers where embryo sacs have reached the stage of the third division. No GUS expression was observed in embryo sacs or pollen of plants infiltrated with an Agrobacterium strain bearing a GUS gene under the control of a gametophyte-specific promoter. To identify the genetic target we used an insertion mutant in which a gene essential for male gametophytic development has been disrupted by a T-DNA bearing a Basta resistance gene (B(R)). In this mutant the B(R) marker is transferred to the progeny only by the female gametes. This mutant was retransformed with a hygromycin resistance marker and doubly resistant plants were selected. The study of 193 progeny of these transformants revealed 25 plants in which the two resistance markers were linked in coupling and only one plant where they were linked in repulsion. These results point to the chromosome set of the female gametophyte as the main target for the T-DNA. PMID:10924482

  9. Monitoring species of arbuscular mycorrhizal fungi in planta and in soil by nested PCR: application to the study of the impact of sewage sludge

    Microsoft Academic Search

    E. Jacquot; D. van Tuinen; S. Gianinazzi; V. Gianinazzi-Pearson

    2000-01-01

    Nested PCR is a highly sensitive procedure for monitoring species of arbuscular mycorrhizal (AM) fungi and for determining\\u000a their abundance in planta and in soil. DNA sequence variability in the D1 and D2 domains of the large ribosomal subunit is sufficient to design primers\\u000a which discriminate between AM fungi at the species level. The usefulness of this molecular approach is

  10. Función neuropsicológica en la fenilcetonuria tratada precozmente: Una revisión

    Microsoft Academic Search

    Louise H. Crossley; Peter J. Anderson

    2010-01-01

    ResumenLa fenilcetonuria (FCU) es un error metabólico congénito que implica una deficiencia de la enzima fenilalanina hidroxilasa. Este proceso da lugar a niveles elevados de fenilalanina y niveles bajos de tirosina. Si no se administra tratamiento aparecen graves secuelas neuropatológicas y neuroconductuales. La puesta en práctica de la detección sistemática de recién nacidos y el tratamiento dietético precoz ha reducido

  11. Diseño reutilizable dentro de una red de objetos de aprendizaje

    Microsoft Academic Search

    Miguel Latorre; Manuel Blázquez; Sergio Martín; Gabriel Díaz; Manuel Castro; Juan Peire; Inmaculada Plaza; Francisco Arcega; Tomas Pollán; Edmundo Tovar; Martín Llamas; Manuel Caeiro

    2009-01-01

    Abstract—Las nuevas tecnologías aplicadas en la enseñanza a distancia suponen un gran avance en la publicación y localización delos contenidos. Herramientas orientadas al educador van a permitir crear contenidos digitales con una riqueza mucho mayor ala,conocida hasta ahora con un coste de mantenimiento inferior. Los estándares educativos, repositorios de materiales didácticos y las plataformas de aprendizaje son los agentes involucrados

  12. Ipotesi per una introduzione alla Meccanica dei fluidi

    E-print Network

    Roma "La Sapienza", Università di

    Ipotesi per una introduzione alla Meccanica dei fluidi Giovanni Gallavotti 4 Roma 1996 20/novembre uniforme, il rosso dei tramonti romani nei ricordi d' infanzia, la stella ottagona centrale e sia nero, il Introduzione Si resta colpiti dalla sostanziale identit´a concettuale dei problemi che si incontrano nello

  13. LAS ESTIMACIONES DEL DESEMPLEO DE EQUILIBRIO. UNA PANORÁMICA

    Microsoft Academic Search

    FRANCISCO GÓMEZ; CARLOS USABIAGA

    En este artículo abordamos una panorámica de las estimaciones del de- sempleo de equilibrio de la economía española realizadas desde mediados de los años ochenta hasta la actualidad. En primer lugar, exponemos sinté- ticamente en qué consisten los métodos más comúnmente empleados para medir el desempleo de equilibrio, señalando sus propiedades y limitacio- nes. Clasificamos los métodos en dos grupos,

  14. Se busca una raíz unitaria: evidencia para Chile

    Microsoft Academic Search

    Rómulo Chumacero Escudero

    Este documento presenta evidencia contundente en contra de la hipótesis nula de que series tales como el IMACEC, el consumo o el PIB chilenos presentan una raíz unitaria. Esta evidencia se apoya en resultados derivados en Chumacero (2000) que muestran que en caso que alguna de estas variables de escala sea estacionaria en diferencia, los retornos de activos disponibles para

  15. Una fusión: ¿Necesidad o estrategia? (caso de estudio)

    Microsoft Academic Search

    Diana Teresa Diez C; Beatriz Eugenia Dussan; Liliana Estrada; Sandra Monsalve

    1998-01-01

    Este caso es real y está teniendo lugar actualmente en el sector financiero colombiano. La globalización de los mercados y la apertura del sector financiero en nuestro país, dieron lugar a una fuerte competencia no sólo entre las instituciones locales sino con la banca extranjera. Como respuesta a la situación del entorno, en el caso particular del Banco Industrial Colombiano

  16. "Come scrivere una proposta Marie Sklodowska-Curie individuale"

    E-print Network

    Di Pillo, Gianni

    "Come scrivere una proposta Marie Sklodowska-Curie individuale" (con particolare riferimento al billion #12;KEY FEATURES of Marie Sklodowska-Curie ACTIONS #12; Encourage new, creative types of training in detail: Marie Sklodowska-Curie ACTIONS #12;Marie Sklodowska-Curie actions Marie Curie actions Marie

  17. Lorica, una colonia árabe a orillas del río Sinú

    Microsoft Academic Search

    Joaquín Viloria De La Hoz

    2003-01-01

    En este artículo se estudian las circunstancias como llegaron los primeros inmigrantes árabes a Colombia desde finales del siglo XIX, así como sus actividades económicas más importantes. La investigación escogió a Lorica como punto de referencia, al ser una de las poblaciones más próspera de la subregión del Sinú durante el siglo XIX y primeras décadas del siguiente, en donde

  18. Deteccion facial basada en una distancia de Hausdorff normalizada

    E-print Network

    Escolano, Francisco

    ´etodos m´as robustos. En un sistema de detecci´on basado en ajuste de modelos, se predefine un patr, Alicante pablo@dccia.ua.es, fpujol@dtic.ua.es, rizo@dccia.ua.es, mar@dccia.ua.es Abstract. Los sistemas de diversas apli- caciones biom´etricas. En este tipo de sistemas, la distancia de Hausdorff es una m

  19. El teatro de los '70: Una dramaturgia sitiada

    E-print Network

    Cosentino, Olga

    1991-04-01

    lastimado o Chau papá), Jorge D'Elía (No dejes de venir a visitamos), Elio Gallipoli (¡Hola hermanitof, El rictus), Marta Gavensky (El gran soñador, El inmortal, La faena), Diego Mileo (Cómo es una solapa, Que el tumulto nos proteja, Qué quiere decir...

  20. Estadística y objetos de aprendizaje. Una experiencia in vivo

    Microsoft Academic Search

    Javier Organista Sandoval; Graciela Cordero Arroyo

    Este texto es la presentación de los resultados preliminares de una investigación-desa- rrollo que tuvo el propósito de realizar lecciones de estadística en línea con inserción de objetos de aprendizaje y estimar el efecto sobre el aprendizaje de los alumnos par- ticipantes en un ambiente natural universitario. Se utilizaron señalamientos teóricos constructivistas para el desarrollo de las lecciones, para conformarle

  1. EL COLEGIO PILOTO DE FUTURO DE MEDELLÍN: UNA INNOVACIÓN EDUCATIVA

    Microsoft Academic Search

    El Colegio Piloto de Futuro de Medellín, CPF, es una innovación educativa con visión prospectiva que utiliza recursos científicos y entornos de aprendizaje que se valen de avanzadas tecnologías informáticas. Los estudiantes se forman mediante el uso creativo de tales tecnologías con la intención expresa de desarrollar su capacidad de abstracción, la creatividad, el cambio dinámico, el respeto por la

  2. A Real-Time Fluorogenic Assay for the Visualization of Glycoside Hydrolase Activity in Planta1[C][OA

    PubMed Central

    Ibatullin, Farid M.; Banasiak, Alicja; Baumann, Martin J.; Greffe, Lionel; Takahashi, Junko; Mellerowicz, Ewa J.; Brumer, Harry

    2009-01-01

    There currently exists a diverse array of molecular probes for the in situ localization of polysaccharides, nucleic acids, and proteins in plant cells, including reporter enzyme strategies (e.g. protein-glucuronidase fusions). In contrast, however, there is a paucity of methods for the direct analysis of endogenous glycoside hydrolases and transglycosidases responsible for cell wall remodeling. To exemplify the potential of fluorogenic resorufin glycosides to address this issue, a resorufin ?-glycoside of a xylogluco-oligosaccharide (XXXG-?-Res) was synthesized as a specific substrate for in planta analysis of XEH activity. The resorufin aglycone is particularly distinguished for high sensitivity in muro assays due to a low pKa (5.8) and large extinction coefficient (? 62,000 m?1cm?1), long-wavelength fluorescence (excitation 571 nm/emission 585 nm), and high quantum yield (0.74) of the corresponding anion. In vitro analyses demonstrated that XXXG-?-Res is hydrolyzed by the archetypal plant XEH, nasturtium (Tropaeolum majus) NXG1, with classical Michaelis-Menten substrate saturation kinetics and a linear dependence on both enzyme concentration and incubation time. Further, XEH activity could be visualized in real time by observing the localized increase in fluorescence in germinating nasturtium seeds and Arabidopsis (Arabidopsis thaliana) inflorescent stems by confocal microscopy. Importantly, this new in situ XEH assay provides an essential complement to the in situ xyloglucan endotransglycosylase assay, thus allowing delineation of the disparate activities encoded by xyloglucan endotransglycosylase/hydrolase genes directly in plant tissues. The observation that XXXG-?-Res is also hydrolyzed by diverse microbial XEHs indicates that this substrate, and resorufin glycosides in general, may find broad applicability for the analysis of wall restructuring by polysaccharide hydrolases during morphogenesis and plant-microbe interactions. PMID:19783642

  3. Identification of Epichloë Endophytes In Planta by a Microsatellite-Based PCR Fingerprinting Assay with Automated Analysis

    PubMed Central

    Moon, Christina D.; Tapper, Brian A.; Scott, Barry

    1999-01-01

    Epichloë endophytes are a group of filamentous fungi that include both sexual (Epichloë) and asexual (Neotyphodium) species. As a group they are genetically diverse and form both antagonistic and mutualistic associations with temperate grasses. We report here on the development of a microsatellite-based PCR system for fingerprinting this group of fungi with template isolated from either culture or infected plant material. M13mp19 partial genomic libraries were constructed for size-fractionated genomic DNA from two endophyte strains. These libraries were screened with a mixture of DIG-labeled dinucleotide and trinucleotide repeat probes. Positive clones were sequenced, and nine unique microsatellite loci were identified. An additional microsatellite was serendipitously identified in the 3? untranscribed region of the 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase gene from N. lolii Lp19. Primers were designed for each locus and a panel of endophytes, from different taxonomic groupings, was screened to determine the degree of polymorphism. On the basis of these results a multiplex assay was developed for strain identification with fluorescently labeled primers for five of these loci. Using this system the size of the products amplified can be precisely determined by automated analysis, and an allele profile for each strain can be readily generated. The assay was shown to resolve endophyte groupings to the level of known isozyme phenotype groupings. In a blind test the assay was used successfully to identify a set of endophytes in planta. A reference database of allele sizes has been established for the panel of endophytes examined, and this will be expanded as new strains are analyzed. PMID:10049893

  4. Pasado, presente y futuro de la epidemiología. Una perspective latinoamericana

    PubMed Central

    Morabia, Alfredo

    2015-01-01

    Este artículo intenta contestar tres preguntas. Sobre el pasado: ¿Por qué no existió una epidemiología precolombina? Sobre el presente: ¿Cuáles son los orígenes de la epidemiología moderna, incluyendo sus raíces sudamericanas? Y sobre el futuro, escogí un título surrealista para enfatizar el hecho que estoy consciente de que es siempre delicado hacer predicciones: ¿Por qué fenómenos complejos son los “objetos oscuros del deseo” epidemiológico? PMID:25124247

  5. [Lichen ruber ulcerosus plantae].

    PubMed

    Huwyler, T; Künzi, W; Dörzapf, O

    1989-01-01

    A 66-year-old patient suffering from painful ulcerative lichen planus of the feet is presented. Various treatments (topical and oral corticosteroids, irradiation and a 6-month therapy with acitretin) were tried without success. Skin grafting in the affected areas was helpful and resulted with an acceptable and painless state. PMID:2921116

  6. Bikinin-like inhibitors targeting GSK3/Shaggy-like kinases: characterisation of novel compounds and elucidation of their catabolism in planta

    PubMed Central

    2014-01-01

    Background Plant GSK-3/Shaggy-like kinases are key players in brassinosteroid (BR) signalling which impact on plant development and participate in response to wounding, pathogens and salt stress. Bikinin was previously identified in a chemical genetics screen as an inhibitor targeting these kinases. To dissect the structural elements crucial for inhibition of GSK-3/Shaggy-like kinases by bikinin and to isolate more potent compounds we synthesised a number of related substances and tested their inhibitory activity in vitro and in vivo using Arabidopsis thaliana. Results A pyridine ring with an amido succinic acid residue in position 2 and a halogen in position 5 were crucial for inhibitory activity. The compound with an iodine substituent in position 5, denoted iodobikinin, was most active in inhibiting BIN2 activity in vitro and efficiently induced brassinosteroid-like responses in vivo. Its methyl ester, methyliodobikinin, showed improved cell permeability, making it highly potent in vivo although it had lower activity in vitro. HPLC analysis revealed that the methyl residue was rapidly cleaved off in planta liberating active iodobikinin. In addition, we provide evidence that iodobikinin and bikinin are inactivated in planta by conjugation with glutamic acid or malic acid and that the latter process is catalysed by the malate transferase SNG1. Conclusion Brassinosteroids participate in regulation of many aspects of plant development and in responses to environmental cues. Thus compounds modulating their action are valuable tools to study such processes and may be an interesting opportunity to modify plant growth and performance in horticulture and agronomy. Here we report the development of bikinin derivatives with increased potency that can activate BR signalling and mimic BR action. Methyliodobikinin was 3.4 times more active in vivo than bikinin. The main reason for the superior activity of methyliodobikinin, the most potent compound, is its enhanced plant tissue permeability. Inactivation of bikinin and its derivatives in planta involves SNG1, which constitutes a novel pathway for modification of xenobiotic compounds. PMID:24947596

  7. La Sede Universitaria de Orihuela presenta de forma indita una exposicin fotogrfica de cazatormentas

    E-print Network

    Escolano, Francisco

    meteorológicos a través de una gran colección de fotos y vídeos de socios de Acamet (Asociación de cazatormentas 60 fotografías distintas y otra de una serie de vídeos de fotos time-lapse para ver la evolución de nubes, cascadas de nubes y creación de tormentas. La selección de las fotos se ha hecho por los propios

  8. UNA ARQUITECTURA SEGMENTADA PARA EL CLCULO DEL TIEMPO AL IMPACTO CON VISIN LOG-POLAR

    E-print Network

    Valencia, Universidad de

    de cerca de 90 imágenes por segundo. 1. Introducción La navegación de plataformas móviles autónomas múltiples métodos de sensorización de una plataforma móvil destaca la sensorización visual por combinar una plataforma móvil tendrá. Esta potencia de cálculo, necesaria para la plataforma móvil, es un problema que

  9. Apertium, una plataforma de cdigo abierto para el desarrollo de sistemas

    E-print Network

    Forcada, Mikel L.

    Apertium, una plataforma de código abierto para el desarrollo de sistemas de traducción automática.org, una plataforma avanzada de código abierto, con licencia GNU GPL, que, gracias al desacoplamiento que plataforma Apertium ha sido desarrollada por el grupo de investi- gación Transducens de la Universitat d

  10. 2 Funcions reals d'una variable 7 2A. Alguns resultats b`asics . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

    E-print Network

    Casanellas, Marta

    ´INDEX 2 Funcions reals d'una variable 7 2A. Alguns resultats b`asics . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2.1 Funcions reals d'una variable . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2.2 L Successions 39 4.1 Generalitats sobre funcions de variable discreta . . . . . . . . . . . . . . . 39 4

  11. PER A UNA PEDAGOGIA DEL LABERINT del text: l'autor, 2008

    E-print Network

    Oro, Daniel

    PER A UNA PEDAGOGIA DEL LABERINT #12;© del text: l'autor, 2008 © de l'edició: Universitat de les Palma DL: PM 1835-2008 #12;PER A UNA PEDAGOGIA DEL LABERINT He triat per a aquesta ocasió reflexionar vostre inclou el camp de la teoria, però també, i en gran manera, el de la pràctica, ja que en pedagogia

  12. Una revolución (no tan) silenciosa. Claves para repensar el agro en Argentina

    Microsoft Academic Search

    Roberto Bisang; Guillermo Anlló; Mercedes Campi

    Introducción La relación entre las producciones agropecuarias y el desarrollo de la economía argentina tiene una larga y compleja historia. En sus orígenes, este sector -en base a una mezcla de expansión de la frontera territorial, la calidad de suelos y climas y la importación\\/adaptación de tecnología, sumado a los recursos humanos- fue el motor del crecimiento aunque con deudas

  13. NEUROTICISMO, EXTRAVERSION Y ESTILO ATRIBUCIONAL EN VETERANOS DE GUERRA: UNA APROXIMACION DESDE EL ESTRES POSTRAUMATICO

    Microsoft Academic Search

    Alejandro Enrique

    Resumen Las personas que experimentan un evento traumático (guerra, desastres naturales, abuso sexual, entre otros), a me- nudo suelen desarrollar un tipo específico de patología clínica conocida como Síndrome de Estrés Postraumático que fre- cuentemente está asociada a una serie de trastornos con una fuerte incidencia negativa en la mayoría de los casos, en todos los aspectos de la vida

  14. ‘He Xie She Hui’, una sociedad armoniosa ¿Es posible la responsabilidad social corporativa en China?

    Microsoft Academic Search

    Pablo Nieto Martínez

    2010-01-01

    Desde 2006, la doctrina oficial del Partido Comunista Chino persigue la creación de una “sociedad armoniosa” (hexie shehui). El liderazgo de Hu Jintao reconoce así los nuevos problemas asociados a la extraordinaria expansión económica china. La economía crece, pero crecen también las tensiones derivadas de una mayor desigualdad social, daños medioambientales, problemas de salud pública, la diversidad étnica y los

  15. Una gua de navegacin para las familias de Wisconsin con nios y

    E-print Network

    Wisconsin at Madison, University of

    Una guía de navegación para las familias de Wisconsin con niños y jóvenes con necesidades especiales de salud y discapacidades. GUÍA DE RECURSOS #12;#12;1 GUÍA DE RECURSOS Una guía de navegación para las familias de Wisconsin con niños y jóvenes con necesidades especiales de salud y discapacidades

  16. Aportaciones del carlismo valenciano a la creación de una derecha movilizadora en los años treinta

    Microsoft Academic Search

    Rafael Valls

    2000-01-01

    La capacidad de adaptación al nuevo orden social de los herederos valencianos del carlismo tuvo una de sus claras manifestaciones en los años treinta. Sin dejar de mantener una política conservadora y con pocas concesiones al populismo, la Derecha Regional Valenciana supo desbancar a los partidos dinásticos tradicionales, absorbiendo a gran parte de sus dirigentes y afiliados, y logrando casi

  17. El management japonés: Una revisión de su literatura. Parte I: Conceptos y teorías

    Microsoft Academic Search

    Enrique Yacuzzi

    2006-01-01

    Presentamos la Parte I de una introducción al management japonés, basada en una revisión de la literatura, que describe conceptos y teorías. Examinamos “los tres tesoros”: el empleo vitalicio, el seniority system y los sindicatos por empresa; también mencionamos otros rasgos del sistema, como las decisiones por consenso y los mecanismos de ‘voice’ (antes que de ‘exit’), junto a características

  18. E-STAKEHOLDERS: UNA APLICACIÓN DE LA TEORÍA DE LOS STAKEHOLDER A LOS NEGOCIOS ELECTRÓNICOS

    Microsoft Academic Search

    CARLOS HERNÁN GONZÁLEZ CAMPO

    2010-01-01

    En los negocios electrónicos la masifi cación de la personalización o mass customization (por su traducción al inglés), es la principal estrategia de las organizaciones virtuales en su relación con los stakeholders (empleados, accionistas, proveedores y socios), para transformar su identificación, reconocimiento y gestión, de una forma muy diferente a la de una organización tradicional. El presente artículo de reflexión

  19. Una Evaluación Geográfica de la Política de Educación Media Superior de la Ciudad de México

    Microsoft Academic Search

    LAURA CERVANTES-SALCIDO; CARLOS J. VILALTA-Y PERDOMO

    2006-01-01

    En este trabajo presentamos una evaluación geográfica de la política de las preparatorias creadas y administradas por el gobierno de la ciudad de México. La revisión teórica y evaluación estadística realizadas nos ha permitido (1) construir relaciones explicativas útiles entre varios conceptos de teorías urbanas provenientes de escuelas divergentes, y de disciplinas diferentes, y a la vez (2) hacer una

  20. Desarrollo y cultura: Origenes y tendencias recientes de una relacion indispensable

    Microsoft Academic Search

    Aaron Espinosa Espinosa; Augusto Alean Pico; Alberto Abello Vives

    2011-01-01

    ¿De que trata el desarrollo? Ha sido una pregunta fundamental en la historia de la ciencia economica, y aun sigue siendo una pregunta central en las ciencias sociales. Desde sus inicios la teoria economica dejo por fuera a las actividades culturales. Adam Smith asimilaba la cultura como esencia del trabajo no productivo, y otro importante economista, Alfred Marshall, mostraba la

  1. Dos médicos y una causa: Len Crome y Reginald Saxton en las Brigadas Internacionales

    Microsoft Academic Search

    Paul Preston

    2004-01-01

    Una de las dimensiones más impresionantes, más duraderas y menos conocidas de las Brigadas Internacionales era la aportación de sus servidos médicos. Las aportaciones de los distintos médicos, españoles e internacionales, fueron de una importancia colosal en el desarrollo posterior de la medicina traumatològica, en guerra y en paz. Menos conocida fue la labor abnegada de conductores de ambulancias, enfermeras

  2. Una medición del impacto del Programa de Capacitación Laboral Juvenil PROJoven

    Microsoft Academic Search

    Hugo Ñopo; Miguel Robles; Jaime Saavedra

    2002-01-01

    El presente documento presenta una medición de impacto del programa de capacitación laboral juvenil PROJoven. Se utilizó información socio-laboral de una muestra de beneficiarios y un conjunto de individuos que conformaron el grupo de control. Esta información fue recogida antes de iniciados los cursos de capacitación de la sexta convocatoria (línea de base) y después de seis meses de concluido

  3. Reconexión magnética en una región activa en decaimiento

    NASA Astrophysics Data System (ADS)

    Mandrini, C. H.; Démoulin, P.; van Driel-Gesztelyi, L.; Schmieder, B.; Bagalá, L. G.; Rovira, M. G.

    Se han obtenido observaciones desde Tierra (H? y magnetogramas) coordinadas con el Soft X-ray Telescope (SXT), a bordo del satélite japonés Yohkoh, de una región activa bipolar en decaimiento. Estos datos constituyen la base para el estudio de un punto brillante en rayos X (PBX) y de la actividad relacionada con el mismo en distintas capas de la atmósfera solar. Las observaciones muestran que el PBX está relacionado con la aparición de un bipolo menor (~ 1020 Mx) y que su abrillantamiento continuo, así como sus aumentos de brillo esporádicos (``fulguraciones"), son el resultado de la reconexión entre el pequeño arco emergente (visto en H? como un sistema de filamentos arqueados, SFA) y arcos mayores asociados al campo facular preexistente. Se ha extrapolado el campo magnético observado en la aproximación libre de fuerzas lineal y se ha seguido su evolución a lo largo de la vida del PBX. Se ha calculado la posición de las cuasiseparatrices (CSs) a partir del campo modelado. Las líneas de campo extrapoladas, cuyas bases fotosféricas se encuentran a ambos lados de las CSs, están de acuerdo con las estructuras cromosféricas y coronales observadas. Se ha calculado el espesor de la CD ubicada a lo largo de la polaridad negativa emergente, encontrándose que su variación está de acuerdo con la evolución de la intensidad del PBX; la CS es muy delgada durante la vida del PBX (~ 100 m), mientras que su espesor aumenta considerablemente (>= 10^4 m) cuando el PBX desaparece de las imágenes del SXT. Estos resultados señalan que el abrillantamiento y las ``fulguraciones" de este PBX se deben al proceso de reconexión en 3D que tiene lugar en las CSs.

  4. Arabidopsis DAYU/ABERRANT PEROXISOME MORPHOLOGY9 Is a Key Regulator of Peroxisome Biogenesis and Plays Critical Roles during Pollen Maturation and Germination in Planta[W

    PubMed Central

    Li, Xin-Ran; Li, Hong-Ju; Yuan, Li; Liu, Man; Shi, Dong-Qiao; Liu, Jie; Yang, Wei-Cai

    2014-01-01

    Pollen undergo a maturation process to sustain pollen viability and prepare them for germination. Molecular mechanisms controlling these processes remain largely unknown. Here, we report an Arabidopsis thaliana mutant, dayu (dau), which impairs pollen maturation and in vivo germination. Molecular analysis indicated that DAU encodes the peroxisomal membrane protein ABERRANT PEROXISOME MORPHOLOGY9 (APEM9). DAU is transiently expressed from bicellular pollen to mature pollen during male gametogenesis. DAU interacts with peroxisomal membrane proteins PEROXIN13 (PEX13) and PEX16 in planta. Consistently, both peroxisome biogenesis and peroxisome protein import are impaired in dau pollen. In addition, the jasmonic acid (JA) level is significantly decreased in dau pollen, and the dau mutant phenotype is partially rescued by exogenous application of JA, indicating that the male sterility is mainly due to JA deficiency. In addition, the phenotypic survey of peroxin mutants indicates that the PEXs most likely play different roles in pollen germination. Taken together, these data indicate that DAU/APEM9 plays critical roles in peroxisome biogenesis and function, which is essential for JA production and pollen maturation and germination. PMID:24510720

  5. Mutations in the Capsid Protein of Brome Mosaic Virus Affecting Encapsidation Eliminate Vesicle Induction In Planta: Implications for Virus Cell-to-Cell Spread

    PubMed Central

    Bamunusinghe, Devinka; Chaturvedi, Sonali; Seo, Jang-Kyun

    2013-01-01

    Positive-strand RNA viruses are known to rearrange the endomembrane network to make it more conducive for replication, maturation, or egress. Our previous transmission electron microscopic (TEM) analysis showed that ectopic expression of wild-type (wt) capsid protein (CP) of Brome mosaic virus (BMV) has an intrinsic property of modifying the endoplasmic reticulum (ER) to induce vesicles similar to those present in wt BMV infection. In this study, we evaluated the functional significance of CP-mediated vesicle induction to the BMV infection cycle in planta. Consequently, the cytopathologic changes induced by wt CP or its mutants defective in virion assembly due to mutations engineered in either N- or C-proximal domains were comparatively analyzed by TEM in two susceptible (Nicotiana benthamiana and Chenopodium quinoa) and one nonhost (N. clevelandii) plant species. The results showed that in susceptible hosts, CP-mediated ER-derived vesicle induction is contingent on the expression of encapsidation-competent CP. In contrast, unlike in N. benthamiana and C. quinoa, transient expression of wt CP in nonhost N. clevelandii plants eliminated vesicle induction. Additionally, comparative source-to-sink analysis of virus spread in leaves of N. benthamiana and N. clevelandii coexpressing wt BMV and Cucumber mosaic virus (CMV) showed that despite trans-encapsidation, CMV failed to complement the defective cell-to-cell movement of BMV. The significance and relation of CP-mediated vesicle induction to virus cell-to-cell movement are discussed. PMID:23741003

  6. Mutations in the capsid protein of Brome mosaic virus affecting encapsidation eliminate vesicle induction in planta: implications for virus cell-to-cell spread.

    PubMed

    Bamunusinghe, Devinka; Chaturvedi, Sonali; Seo, Jang-Kyun; Rao, A L N

    2013-08-01

    Positive-strand RNA viruses are known to rearrange the endomembrane network to make it more conducive for replication, maturation, or egress. Our previous transmission electron microscopic (TEM) analysis showed that ectopic expression of wild-type (wt) capsid protein (CP) of Brome mosaic virus (BMV) has an intrinsic property of modifying the endoplasmic reticulum (ER) to induce vesicles similar to those present in wt BMV infection. In this study, we evaluated the functional significance of CP-mediated vesicle induction to the BMV infection cycle in planta. Consequently, the cytopathologic changes induced by wt CP or its mutants defective in virion assembly due to mutations engineered in either N- or C-proximal domains were comparatively analyzed by TEM in two susceptible (Nicotiana benthamiana and Chenopodium quinoa) and one nonhost (N. clevelandii) plant species. The results showed that in susceptible hosts, CP-mediated ER-derived vesicle induction is contingent on the expression of encapsidation-competent CP. In contrast, unlike in N. benthamiana and C. quinoa, transient expression of wt CP in nonhost N. clevelandii plants eliminated vesicle induction. Additionally, comparative source-to-sink analysis of virus spread in leaves of N. benthamiana and N. clevelandii coexpressing wt BMV and Cucumber mosaic virus (CMV) showed that despite trans-encapsidation, CMV failed to complement the defective cell-to-cell movement of BMV. The significance and relation of CP-mediated vesicle induction to virus cell-to-cell movement are discussed. PMID:23741003

  7. In Planta Stage-Specific Fungal Gene Profiling Elucidates the Molecular Strategies of Fusarium graminearum Growing inside Wheat Coleoptiles[W][OA

    PubMed Central

    Zhang, Xiao-Wei; Jia, Lei-Jie; Zhang, Yan; Jiang, Gang; Li, Xuan; Zhang, Dong; Tang, Wei-Hua

    2012-01-01

    The ascomycete Fusarium graminearum is a destructive fungal pathogen of wheat (Triticum aestivum). To better understand how this pathogen proliferates within the host plant, we tracked pathogen growth inside wheat coleoptiles and then examined pathogen gene expression inside wheat coleoptiles at 16, 40, and 64 h after inoculation (HAI) using laser capture microdissection and microarray analysis. We identified 344 genes that were preferentially expressed during invasive growth in planta. Gene expression profiles for 134 putative plant cell wall–degrading enzyme genes suggest that there was limited cell wall degradation at 16 HAI and extensive degradation at 64 HAI. Expression profiles for genes encoding reactive oxygen species (ROS)–related enzymes suggest that F. graminearum primarily scavenges extracellular ROS before a later burst of extracellular ROS is produced by F. graminearum enzymes. Expression patterns of genes involved in primary metabolic pathways suggest that F. graminearum relies on the glyoxylate cycle at an early stage of plant infection. A secondary metabolite biosynthesis gene cluster was specifically induced at 64 HAI and was required for virulence. Our results indicate that F. graminearum initiates infection of coleoptiles using covert penetration strategies and switches to overt cellular destruction of tissues at an advanced stage of infection. PMID:23266949

  8. In Planta Biocontrol of Pectobacterium atrosepticum by Rhodococcus erythropolis Involves Silencing of Pathogen Communication by the Rhodococcal Gamma-Lactone Catabolic Pathway.

    PubMed

    Barbey, Corinne; Crépin, Alexandre; Bergeau, Dorian; Ouchiha, Asma; Mijouin, Lily; Taupin, Laure; Orange, Nicole; Feuilloley, Marc; Dufour, Alain; Burini, Jean-François; Latour, Xavier

    2013-01-01

    The virulence of numerous Gram-negative bacteria is under the control of a quorum sensing process based on synthesis and perception of N-acyl homoserine lactones. Rhodococcus erythropolis, a Gram-positive bacterium, has recently been proposed as a biocontrol agent for plant protection against soft-rot bacteria, including Pectobacterium. Here, we show that the ?-lactone catabolic pathway of R. erythropolis disrupts Pectobacterium communication and prevents plant soft-rot. We report the first characterization and demonstration of N-acyl homoserine lactone quenching in planta. In particular, we describe the transcription of the R. erythropolis lactonase gene, encoding the key enzyme of this pathway, and the subsequent lactone breakdown. The role of this catabolic pathway in biocontrol activity was confirmed by deletion of the lactonase gene from R. erythropolis and also its heterologous expression in Escherichia coli. The ?-lactone catabolic pathway is induced by pathogen communication rather than by pathogen invasion. This is thus a novel and unusual biocontrol pathway, differing from those previously described as protecting plants from phytopathogens. These findings also suggest the existence of an additional pathway contributing to plant protection. PMID:23805254

  9. High-throughput in planta expression screening identifies a class II ethylene-responsive element binding factor-like protein that regulates plant cell death and non-host resistance

    Microsoft Academic Search

    Khairun Hisam Bin Nasir; Yoshihiro Takahashi; Akiko Ito; Hiromasa Saitoh; Hideo Matsumura; Hiroyuki Kanzaki; Takeshi Shimizu; Minako Ito; Shizuko Fujisawa; Prakash C. Sharma; Masaru Ohme-Takagi; Sophien Kamoun; Ryohei Terauchi

    2005-01-01

    Summary We performed high-throughput screening using the potato virus X (PVX) system to overexpress Nicotiana benthamiana genes in planta and identify positive regulators of cell death. This screening identified NbCD1 ,a novel class II ethylene-responsive element binding factor (ERF), as a potent inducer of the hypersensitive response (HR)-like cell death. NbCD1 expression was induced by treatments with INF1 elicitor and

  10. La Dama de Elche es una urna funeraria. Alejandro Ramos Molina.

    E-print Network

    Escolano, Francisco

    superficial de yeso cubre toda la escultura y sirve de base para su policromía. Este estucado también ha sido temperaturas y cambios de humedad que disolvieron y recristalizaron el yeso del estucado, lo que permitió una

  11. CORPOREIDAD Y MOTRICIDAD. UNA FORMA DE MIRAR LOS SABERES DEL CUERPO

    Microsoft Academic Search

    RENE HURTADO HERRERA

    RESÚMEN: Corporeidad y motricidad no son simples distinciones utilizadas para refinar el lenguaje, son dos conceptos de tradición fenomenológica que han empezado a ser utilizados en los últimos tiempos en el contexto de una nueva \\

  12. PRCTICA DE PROBLEMAS E INFORMTICA PRCTICA 5. ELABORACIN DE UNA RUTA TURSTICA TEMTICA

    E-print Network

    Escolano, Francisco

    a lugares de interés a lo largo del tiempo de viaje. - Como mínimo, se incluirá una ruta turística a pié aspectos de interés en la planificación de un viaje turístico. En los últimos años estamos asistiendo a un

  13. Curs de Càlcul: una nova metodologia amb Moodle com a excusa

    Microsoft Academic Search

    M. Rosa Estela Carbonell

    2009-01-01

    El projecte que es presenta, consisteix bàsicament en la elaboració de material docent d’autoaprenentatge utilitzant els diferents recursos de la plataforma Moodle, per tal de desenvolupar un conjunt de temes d’una manera diferent a com es presenten habitualment als estudiants i així poder millorar i consolidar els coneixements de l’estudiant en aquests temes. \\u000aUna de les motivacions principals que ens

  14. Sulla Probabilità Termodinamica di Stato di una Massa D’aria

    Microsoft Academic Search

    Antonio Pignedoli

    1942-01-01

    Sunto  L’A., applicando il concetto di probabilità termodinamica ad una massa d’aria e ricollegandosi al fatto che l’annullarsi dell’entropia\\u000a non si verifica soltanto allo zero assoluto, ma in un intervallo dipendente dalla sostanza in questione ed avente come estremo\\u000a inferiore lo zero assoluto, esprime la probabilità termodinamica di una massa d’aria atmosferica. Scrive anche due formule\\u000a relative, rispettivamente, alla temperatura Potenziale

  15. CAPBL: Una herramienta pedagogica computacional para la ensenanza de la Geometr´ia en secundaria

    Microsoft Academic Search

    Ismael Morales Garay

    Se propone una herramienta computacional para la ensenanza de la geometr´ia en secundaria llamada CAPBL (Computer Assisted Problem-Based Learning), la cual esta basada en un sistema de instruccion llamado PBL (Problem-Based Learning). La herramienta propuesta se compone de un metodo de trabajo que se utiliza en la aplicacion directa de la herramienta, una Biblioteca Interactiva que se utiliza como soporte

  16. A ubiquitin carboxyl extension protein secreted from a plant-parasitic nematode Globodera rostochiensis is cleaved in planta to promote plant parasitism.

    PubMed

    Chronis, Demosthenis; Chen, Shiyan; Lu, Shunwen; Hewezi, Tarek; Carpenter, Sara C D; Loria, Rosemary; Baum, Thomas J; Wang, Xiaohong

    2013-04-01

    Nematode effector proteins originating from esophageal gland cells play central roles in suppressing plant defenses and in formation of the plant feeding cells that are required for growth and development of cyst nematodes. A gene (GrUBCEP12) encoding a unique ubiquitin carboxyl extension protein (UBCEP) that consists of a signal peptide for secretion, a mono-ubiquitin domain, and a 12 amino acid carboxyl extension protein (CEP12) domain was cloned from the potato cyst nematode Globodera rostochiensis. This GrUBCEP12 gene was expressed exclusively within the nematode's dorsal esophageal gland cell, and was up-regulated in the parasitic second-stage juvenile, correlating with the time when feeding cell formation is initiated. We showed that specific GrUBCEP12 knockdown via RNA interference reduced nematode parasitic success, and that over-expression of the secreted Gr(?) (SP) UBCEP12 protein in potato resulted in increased nematode susceptibility, providing direct evidence that this secreted effector is involved in plant parasitism. Using transient expression assays in Nicotiana benthamiana, we found that Gr(?) (SP) UBCEP12 is processed into free ubiquitin and a CEP12 peptide (GrCEP12) in planta, and that GrCEP12 suppresses resistance gene-mediated cell death. A target search showed that expression of RPN2a, a gene encoding a subunit of the 26S proteasome, was dramatically suppressed in Gr(?) (SP) UBCEP12 but not GrCEP12 over-expression plants when compared with control plants. Together, these results suggest that, when delivered into host plant cells, Gr(?) (SP) UBCEP12 becomes two functional units, one acting to suppress plant immunity and the other potentially affecting the host 26S proteasome, to promote feeding cell formation. PMID:23346875

  17. Post-abscission, pre-dispersal seeds of Digitalis purpurea remain in a developmental state that is not terminated by desiccation ex planta

    PubMed Central

    Butler, L. H.; Hay, F. R.; Ellis, R. H.; Smith, R. D.

    2009-01-01

    Background and Aims Seed quality may be compromised if seeds are harvested before natural dispersal (shedding). It has been shown previously that slow or delayed drying can increase potential quality compared with immediate rapid drying. This study set out to investigate whether or not there is a critical moisture content, below which drying terminates maturation events for seeds harvested after mass maturity but before dispersal. Methods Seeds of foxglove (Digitalis purpurea) in the post-abscission pre-dispersal phase were held at between 15 and 95 % RH for 4 or 8 d, with or without re-hydration to 95 % RH for a further 4 d, before drying to equilibrium at 15 % RH. In addition, dry seeds were primed for 48 h at ?1 MPa. Subsequent seed longevity was assessed at 60 % RH and 45 °C. Key Results Rate of germination and longevity were improved by holding seeds at a wide range of humidities after harvest. Longevity was further improved by re-hydration at 95 % RH. Priming improved the longevity of the seeds dried immediately after harvest, but not of those first held at 95 % RH for 8 d prior to drying. Conclusions Maturation continued ex planta in these post-abscission, pre-dispersal seeds of D. purpurea dried at 15–80 % RH at a rate correlated positively with RH (cf. ageing of mature seeds). Subsequent re-hydration at 95 % RH enabled a further improvement in quality. Priming seeds initially stored air-dry for 3 months also allowed maturation events to resume. However, once individual seeds within the population had reached maximum longevity, priming had a negative impact on their subsequent survival. PMID:19136494

  18. Virulence and in?planta movement of Xanthomonas hortorum pv. pelargonii are affected by the diffusible signal factor (DSF)-dependent quorum sensing system.

    PubMed

    Barel, Victoria; Chalupowicz, Laura; Barash, Isaac; Sharabani, Galit; Reuven, Michal; Dror, Orit; Burdman, Saul; Manulis-Sasson, Shulamit

    2015-09-01

    Xanthomonas hortorum pv. pelargonii (Xhp), the causal agent of bacterial blight in pelargonium, is the most threatening bacterial disease of this ornamental worldwide. To gain an insight into the regulation of virulence in Xhp, we have disrupted the quorum sensing (QS) genes, which mediate the biosynthesis and sensing of the diffusible signal factor (DSF). Mutations in rpfF (encoding the DSF synthase) and rpfC (encoding the histidine sensor kinase of the two-component system RfpC/RpfG) and overexpression of rpfF showed a significant reduction in incidence and severity of the disease on pelargonium. Confocal laser scanning microscopy images of inoculated plants with a green fluorescent protein (GFP)-labelled wild-type strain showed that the pathogen is homogeneously dispersed in the lumen of xylem vessels, reaching the apex and invading the intercellular spaces of the leaf mesophyll tissue within 21?days. In contrast, the rpfF and rpfC knockout mutants, as well as the rpfF-overexpressing strain, remained confined to the vicinity of the inoculation site. The rpfF and rpfC mutants formed large incoherent aggregates in the xylem vessels that might interfere with upward movement of the bacterium within the plant. Both mutants also formed extended aggregates under in?vitro conditions, whereas the wild-type strain formed microcolonies. Expression levels of putative virulence genes in?planta were substantially reduced within 48?h after inoculation with the QS mutants when compared with the wild-type. The results presented indicate that an optimal DSF concentration is crucial for successful colonization and virulence of Xhp in pelargonium. PMID:25530086

  19. High-throughput in planta expression screening identifies an ADP-ribosylation factor (ARF1) involved in non-host resistance and R gene-mediated resistance.

    PubMed

    Coemans, Bert; Takahashi, Yoshihiro; Berberich, Thomas; Ito, Akiko; Kanzaki, Hiroyuki; Matsumura, Hideo; Saitoh, Hiromasa; Tsuda, Shinya; Kamoun, Sophien; Sági, László; Swennen, Rony; Terauchi, Ryohei

    2008-01-01

    To identify positive regulators of cell death in plants, we performed a high-throughput screening, employing potato virus X-based overexpression in planta of a cDNA library derived from paraquat-treated Nicotiana benthamiana leaves. The screening of 30,000 cDNA clones enabled the identification of an ADP-ribosylation factor 1 (ARF1) that induces cell death when overexpressed in N. benthamiana. Overexpression of the guanosine diphosphate (GDP)-locked mutant of ARF1 did not trigger cell death, suggesting that ARF1 guanosine triphosphatase (GTPase) activity is necessary for the observed cell death-inducing activity. The ARF1 transcript level increased strongly following treatment with Phytophthora infestans elicitor INF1, as well as inoculation with a non-host pathogen Pseudomonas cichorii in N. benthamiana. In addition, ARF1 was induced in the interaction between the N gene and tobacco mosaic virus (TMV) in Nicotiana tabacum. By contrast, inoculation with the virulent pathogen Pseudomonas syringae pv. tabaci did not affect ARF1 expression in N. benthamiana. Virus-induced gene silencing of ARF1 in N. benthamiana resulted in a stunted phenotype, and severely hampered non-host resistance towards P. cichorii. In addition, ARF1 silencing partially compromised resistance towards TMV in N. benthamiana containing the N resistance gene. By contrast, and in accordance with the ARF1 gene expression profile, silencing of ARF1 transcription did not alter the susceptibility of N. benthamiana towards the pathogen P. syringae pv. tabaci. These results strongly implicate ARF1 in the non-host resistance to bacteria and N gene-mediated resistance in N. benthamiana. PMID:18705881

  20. The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny.

    PubMed

    Schiml, Simon; Fauser, Friedrich; Puchta, Holger

    2014-12-01

    The CRISPR/Cas nuclease is becoming a major tool for targeted mutagenesis in eukaryotes by inducing double-strand breaks (DSBs) at pre-selected genomic sites that are repaired by non-homologous end joining (NHEJ) in an error-prone way. In plants, it could be demonstrated that the Cas9 nuclease is able to induce heritable mutations in Arabidopsis thaliana and rice. Gene targeting (GT) by homologous recombination (HR) can also be induced by DSBs. Using a natural nuclease and marker genes, we previously developed an in planta GT strategy in which both a targeting vector and targeting locus are activated simultaneously via DSB induction during plant development. Here, we demonstrate that this strategy can be used for natural genes by CRISPR/Cas-mediated DSB induction. We were able to integrate a resistance cassette into the ADH1 locus of A. thaliana via HR. Heritable events were identified using a PCR-based genotyping approach, characterised by Southern blotting and confirmed on the sequence level. A major concern is the specificity of the CRISPR/Cas nucleases. Off-target effects might be avoided using two adjacent sgRNA target sequences to guide the Cas9 nickase to each of the two DNA strands, resulting in the formation of a DSB. By amplicon deep sequencing, we demonstrate that this Cas9 paired nickase strategy has a mutagenic potential comparable with that of the nuclease, while the resulting mutations are mostly deletions. We also demonstrate the stable inheritance of such mutations in A. thaliana. PMID:25327456

  1. In planta assessment of the role of thioredoxin h proteins in the regulation of S-locus receptor kinase signaling in transgenic Arabidopsis.

    PubMed

    Yamamoto, Masaya; Nasrallah, June B

    2013-11-01

    The self-incompatibility (SI) response of the Brassicaceae is mediated by allele-specific interaction between the stigma-localized S-locus receptor kinase (SRK) and its ligand, the pollen coat-localized S-locus cysteine-rich protein (SCR). Based on work in Brassica spp., the thioredoxin h-like proteins THL1 and THL2, which interact with SRK, have been proposed to function as oxidoreductases that negatively regulate SRK catalytic activity. By preventing the spontaneous activation of SRK in the absence of SCR ligand, these thioredoxins are thought to be essential for the success of cross pollinations in self-incompatible plants. However, the in planta role of thioredoxins in the regulation of SI signaling has not been conclusively demonstrated. Here, we addressed this issue using Arabidopsis thaliana plants transformed with the SRKb-SCRb gene pair isolated from self-incompatible Arabidopsis lyrata. These plants express an intense SI response, allowing us to exploit the extensive tools and resources available in A. thaliana for analysis of SI signaling. To test the hypothesis that SRK is redox regulated by thioredoxin h, we expressed a mutant form of SRKb lacking a transmembrane-localized cysteine residue thought to be essential for the SRK-thioredoxin h interaction. We also analyzed transfer DNA insertion mutants in the A. thaliana orthologs of THL1 and THL2. In neither case did we observe an effect on the pollination responses of SRKb-expressing stigmas toward incompatible or compatible pollen. Our results are consistent with the conclusion that, contrary to their proposed role, thioredoxin h proteins are not required to prevent the spontaneous activation of SRK in the A. thaliana stigma. PMID:24077073

  2. Salinity-induced accumulation of organic osmolytes in barley and wheat leaves correlates with increased oxidative stress tolerance: in planta evidence for cross-tolerance.

    PubMed

    Puniran-Hartley, Norhawa; Hartley, Joseph; Shabala, Lana; Shabala, Sergey

    2014-10-01

    Salinity tolerance in plants is dependent on their abilities to adjust osmotically to reduced soil water potential and to keep intracellular ROS levels under control. Both these processes are believed to rely on de novo synthesis of organic osmolytes (traditionally defined as compatible solutes). However direct in planta evidence for anti-oxidant roles of compatible solutes are scarce. In this work, we induced changes in the level of endogenous organic osmolytes by exposing plants to various levels of NaCl (salinity stress; 50-300 mM range) and then studying sensitivity of leaves to oxidative (UV-B) stress. Increase in the external NaCl concentrations was accompanied by the progressive accumulation in leaf Na(+). This accumulation was much higher in old leaves compared with young ones. In old leaves, three major inorganic ions (Na(+), Cl(-) and K(+)) have made 67.7% and 70.4% of leaf osmotic potential (in wheat and barley, respectively) when exposed to 200 mM NaCl treatment, while in young leaves their contribution was only 43.9% and 46.8%, respectively. Here, organic osmolytes played a substantial role in leaf osmotic adjustment. Increased accumulation of organic osmolytes correlated strongly with activity of PSII in leaves exposed to oxidation inducing UV-B treatment in both species (R(2) = 0.50 for wheat and 0.71 for barley). We conclude that salinity-induced accumulation of organic osmolytes in barley and wheat leaves correlates with increased oxidative stress tolerance and provides the evidence for a mechanism of cross-tolerance between these two stresses. PMID:25068241

  3. Endogenous silencing of Puccinia triticina pathogenicity genes through in planta-expressed sequences leads to the suppression of rust diseases on wheat.

    PubMed

    Panwar, Vinay; McCallum, Brent; Bakkeren, Guus

    2013-02-01

    Rust fungi are destructive plant pathogens. The draft genomes of several wheat-infecting species have been released and potential pathogenicity genes identified through comparative analyses to fungal pathogens that are amenable to genetic manipulation. Functional gene analysis tools are needed to understand the infection process of these obligate parasites and to confirm whether predicted pathogenicity genes could become targets for disease control. We have modified an Agrobacterium tumefaciens-mediated in planta-induced transient gene silencing (PITGS) assay for use in Triticum spp. (wheat), and used this assay to target predicted wheat leaf rust fungus, Puccinia triticina (Pt) pathogenicity genes, a MAP kinase (PtMAPK1), a cyclophilin (PtCYC1) and calcineurin B (PtCNB), to analyze their roles in disease. Agroinfiltration effectively delivered hairpin silencing constructs in wheat, leading to the generation of fungal gene-specific siRNA molecules in infiltrated leaves, and resulting in up to 70% reduction in transcription of the endogenous target genes in superinfected Pt. In vivo silencing caused severe disease suppression, compromising fungal growth and sporulation, as viewed by confocal microscopy and measured by reductions in fungal biomass and emergence of uredinia. Interestingly, using the same gene constructs, suppression of infection by Puccinia graminis and Puccinia striiformis was also achieved. Our results show that A. tumefaciens-mediated PITGS can be used as a reverse-genetics tool to discover gene function in rust fungi. This proof-of-concept study indicates that the targeted fungal transcripts might be important in pathogenesis, and could potentially be used as promising targets for developing RNA interference-based resistance against rust fungi. PMID:23110316

  4. Kingdom Plantae Douglas firs (left);

    E-print Network

    Brown, Christopher A.

    -living and photosynthetic #12;5 Clockwise from upper left: a fern; a club moss; a whisk fern; a horsetail Fern Life Cycle major groups... True mosses (~14,000 species) Liverworts (~8500 species) Hornworts (~200 species) Group I: Non-Vascular Plants Examples of Mosses #12;4 Liverworts and Hornworts Sporophytes of a moss Group

  5. Una dulce bondad que atempera las crueldades: El campo de Griselda Gambaro

    E-print Network

    Picó n Garfield, Evelyn

    1980-07-01

    tetiza en forma de un "Decálogo de la perfecta crueldad."12 I. La crueldad es la mutilación de un modo de vivir (p. 26). II. Hay una relación de poder e impotencia, de agresividad y de pasi vidad. III. La diferencia entre el poderoso y el impotente... Franco. Emma acepta pasivamente su situación de víctima y hasta la niega, encu briendo la cruel mutilación de su vida. De vez en cuando deja escapar un comentario que transparenta su conciencia con una realidad que ella misma falsifica con mentiras y...

  6. BigBlueButton, una alternativa de cdigo abierto para la comunicacin interactiva en actividades educativas

    E-print Network

    Grimaldo, Francisco

    educativas Francisco Grimaldo, Ariadna Fuertes, Máximo Cobos, Miguel Arevalillo Carlos Pérez, Paloma Moreno utilización de una alternativa de código abierto en diversas actividades educativas que requieren comuni para la realiza- ción de actividades educativas, aunque también refle- jan alguna carencia frente

  7. CANARIAS-URUGUAY: UNA DIMENSIN POCO CONOCIDA DEL MOVIMIENTO MIGRATORIO Fernando CARNERO LORENZO

    E-print Network

    Paris-Sud XI, Université de

    CANARIAS-URUGUAY: UNA DIMENSIÓN POCO CONOCIDA DEL MOVIMIENTO MIGRATORIO ISLEÑO Fernando CARNERO, Venezuela, fueron los lugares de arribada preferidos por la diáspora canaria. Pero, Uruguay fue el destino trabajo, Migración, Canarias, Uruguay. #12;1 Introducción1 La historiografía sobre la emigración canaria

  8. PRODOTTI INFINITI Data una famiglia {Y : } di spazi topologici, consideriamo il prodotto cartesiano Y = Y e

    E-print Network

    Marconi, Umberto

    PRODOTTI INFINITI Data una famiglia {Y : } di spazi topologici, consideriamo il prodotto cartesiano Y = Y e la famiglia delle proiezioni {pr : }. La topologia prodotto su Y `e la topologia meno sottoinsiemi finiti di e W fra gli aperti di Y. Proposizione 1 La famiglia di tutti gli insiemi della forma W

  9. Cultivating "Una Persona Educada: A Sentipensante" (Sensing/Thinking) Vision of Education

    ERIC Educational Resources Information Center

    Rendon, Laura I.

    2011-01-01

    This essay focuses on the need to educate the new "persona educada", a dignified, honorable person with a good measure of social and personal responsibility who also possesses the habits of the mind and heart. To cultivate "una persona educada" requires a newly formed vision of education and pedagogy. Examples of three entrenched agreements that…

  10. Proceso de Evaluación de Desempeño por Competencias en una Empresa Financiera Privada de Lima Metropolitana

    Microsoft Academic Search

    Jiza Zárate Amayo

    El presente estudio se enmarca dentro de la investigación aplicada. Se evaluó a 453 trabajadores de una empresa financiera divididos en dos grupos ocupacionales: administrativos (n=426) y ejecutivos (n=27) que comprenden once gerencias cada uno. El instrumento midió nueve competencias: Eficiencia del Trabajo, Conocimientos del Trabajo, Iniciativa y Creatividad, Efectividad en la Solución de Problemas, Sensibilidad hacia el Cliente, Trabajo

  11. Eco-región Eje Cafetero: una experiencia de desarrollo regional en Colombia

    Microsoft Academic Search

    Oscar Arango Gaviria

    1970-01-01

    Con la expedición de la Ley 388 de 1997, se inicia un nuevo proceso de planificación y desarrollo territorial en Colombia. Los preceptos contemplados en dicha Ley, aunados a procesos político-institucionales en la Eco-región Eje Cafetero, han permitido efectuar propuestas interesantes para su desarrollo regional. En este contexto, el artículo realiza una síntesis de algunos de los elementos que han

  12. La Temporalidad en los Sistemas de Recomendacion: Una revision actualizada de

    E-print Network

    , 28049, Madrid, Espa~na 2 Departamento de Sistemas de Informaci´on, Universidad del B´io-B´io, AvdaLa Temporalidad en los Sistemas de Recomendaci´on: Una revisi´on actualizada de propuestas te por sistemas de recomendaci´on, c´omo muestran diversos estudios. Ex- isten diversas propuestas en la

  13. MERCADOS CLÁSICOS: UNA APLICACIÓN EN ESTRUCTURA DE CONJUNTOS Y LÓGICA DIFUSA

    Microsoft Academic Search

    Oscar Andrés Espinosa A; Juan Fernando García B

    2011-01-01

    Esta investigación tiene por finalidad una aplicación de la teoría microeconómica a los conceptos fundamentales del economista clásico Richard Cantillon, explicando cómo los mercados pueden hacer converger o divergir los precios a sus niveles naturales, dependiendo esto, de los procesos de aprendizaje evolutivos que tengan sus agentes (productores y consumidores) a través del tiempo. Se utilizarán conceptos de teoría de

  14. Sistema de información ferroviaria por teléfono: propuesta de una metodología de diseño de gestores de diálogo

    Microsoft Academic Search

    R. San-Segundo; J. M. Montero; J. Ferreiros; J. Macías-Guarasa; J. M. Pardo

    Resumen: En esta contribución se describen las fases llevadas a cabo en el desarrollo un servicio de información ferroviaria en castellano, presentándose una nueva metodología para el diseño de gestores de diálogo en servicios automáticos de información. En esta metodología se propone la combinación de diferentes fuentes de información: intuición, observación y simulación, para definir y evaluar alternativas de diseño.

  15. La identidad profesional: una cualidad esencial para la formación inicial del psicopedagogo

    Microsoft Academic Search

    Ada Yunia Oliva Feria

    2011-01-01

    La formación inicial contemporánea del psicopedagogo es una prioridad en las Universidades de Ciencias Pedagógicas, dada por la implicación social que ejerce este en la orientación y asesoría educativa a estudiantes, familiares, y vecinos de la comunidad entre otras funciones inherentes al Licenciado en Educación, especialidad Pedagogía-Psicología. Para contribuir con la formación de un especialista de excelencia en estas disciplinas

  16. Historia de la contabilidad: Una revisión de las perspectivas tradicionales y críticas de historiografía contable

    Microsoft Academic Search

    Fabian Leonardo Quinche Martín

    2007-01-01

    Resumen La importancia que ha tomado la historia como disciplina en el último siglo tiene una de sus raíces en la tensión existente entre las corrientes tradicionales, y las vertientes críticas o de la 'nueva historia'. Esta discusión se ha trasladado a la disciplina contable otorgándonos en la actualidad múltiples facetas que igualmente se pueden clasificar en la corriente tradicional

  17. Construccin de un ndice de regionalizacin para el Sistema Nacional de Propiedad Industrial (SPI): una

    E-print Network

    Paris-Sud XI, Université de

    Construcción de un índice de regionalización para el Sistema Nacional de Propiedad Industrial (SPI Propiedad Industrial (SPI): una aproximación desde la metodología de componentes principales Jacobo Campo responsable del Sistema de Propiedad Industrial (SPI) en Colombia reconoce la importancia económica y

  18. Los contenidos curriculares en una escuela de primaria innovadora. Entre lo previsto y lo improvisado

    Microsoft Academic Search

    Rafael Feito

    Resumen Este texto procede de un trabajo de campo más amplio, basado en la observación partici- pante y en grupos de discusión y entrevistas en profundidad, en el que el autor analiza el fun- cionamiento de un centro público de Educación Infantil y Primaria que lleva a cabo una inno- vación educativa. En este artículo se describen y analizan los

  19. Cooperativismo, autoayuda y autogestión: Una alternativa uruguaya para la vivienda de interés social

    Microsoft Academic Search

    Graciela Laura Aristondo Martín

    2007-01-01

    Desde su surgimiento en la segunda mitad de los años 60, las cooperativas de vivienda por ayuda mutua del Uruguay han demostrado ser una experiencia de gran impacto en la producción social del hábitat popular urbano. El grado de organización alcanzado durante la etapa de ayuda mutua se extiende a la etapa posterior de la construcción de las viviendas, a

  20. LA OBRA INNOVATIVA DE CARMEN NARANJO: UNA LITERATURA ABIERTA AL FUTURO

    Microsoft Academic Search

    Dra. Ruth; Lorraine Budd

    Resumen La literatura de Carmen Naranjo es tan relevante para el principio de un nuevo milenio, como cuando fue concebida durante las últimas cuatro décadas. Usando motivos como la mutilación, el hogar distópico y las relaciones familiares distorsionadas, doña Carmen retrata la deshumanización y marginalización sentidas en cualquier época por la gente que vive en una sociedad en ruptura y

  1. DISTRIBUCI~NY ABUNDANCIA DE LAS AVES EN LA PEN~NSULAIBERICA. UNA APROXIMACI~N

    E-print Network

    Carrascal, Luis M.

    sobre su avifauna. La distribuci6n geo- grafica de las aves en Iberia es el resultado de la interacci6n asociada hacia el SW. Estos cambios paleoclimhticos han tenido una enorme trascendencia sobre la avifauna Cnfasis en identificar el carhcter inC- dito de la avifauna ibdrica y la importancia de la Penlnsula en el

  2. Tratamiento Quirúrgico de los Meningiomas del Foramen Óptico, Técnicay Resultados de una Serie de 18 Pacientes

    PubMed Central

    Goldschmidt, Ezequiel; Ajler, Pablo; Campero, Álvaro; Landriel, Federico; Sposito, Maximiliano; Carrizo, Antonio

    2014-01-01

    Introducción: los meningiomas del foramen óptico producen un rápido deterioro de la función visual aún cuando su tamaño es pequeño, por eso su diagnóstico y manejo difiere del resto de los meningiomas clinoideos. El propósito de este estudio es presentar la técnica y los resultados de nuestro manejo quirúrgico de meningiomas foraminales (MF). Pacientes y Métodos: se llevó a cabo una revisión de las historias clínicas de 47 pacientes con meningiomas primarios intraorbitarios. Se realizaron 52 cirugías en los pacientes con MF. Se empleó una craneotomía fronto-orbitaria, seguida de una descompresión extradural del canal óptico, resección del componente intraorbitario y exploración intradural del nervio óptico. Resultados: de los 12 pacientes con MF que presentaban la visión conservada, la agudeza visual fue preservada en 7 casos, mejoró en 2, y empeoró en 3. En 18 pacientes, el principal síntoma fue exoftalmos y en 35 pacientes ceguera unilateral. Ocurrieron 6 recurrencias, 2 a 10 años después de la resección quirúrgica. Cinco de ellos fueron reoperados. Se indicó radioterapia después de la recurrencia en 3 pacientes. Conclusión: el manejo de los MF continúa siendo controvertido y frecuentemente se propone un tratamiento conservador. Basados en nuestros hallazgos de frecuente extensión intracraneal, proponemos realizar una resección total o subtotal del tumor, preservando el nervio óptico en pacientes con visión prequirúrgica conservada. PMID:25165616

  3. Sepsis por Salmonella enteritidis en una paciente con lupus eritematoso sistémico

    Microsoft Academic Search

    Carlos Alberto Cañas; Gustavo Adolfo Ospina; María Elena Ochoa; Juan Diego Vélez

    Resumen Presentamos el caso clínico de una paciente de 28 años de edad con Lupus Eritematoso Sistémico (LES), con compromiso a nivel neurológico central, riñón y sistema hematológico, con tratamiento cró- nico de esteroides y desnutrición avanzada, quien desarrolla sepsis por Salmonella enteritidis, germen aislado en muestras tomadas en lavado bronco alveolar, sangre y materia fecal. Presentó un absce- so

  4. Batkoa apiculata (Thaxter) Humber affecting Anopheles (Diptera: Culicidae) in the municipality of Una, Southern Bahia, Brazil

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Surveys for fungal pathogens affecting adult mosquitoes from the genus Anopheles were conducted in flooded and swamp-like natural breeding sites near residences in the center and suburbs of the city of Una as well as the nearby village of Outeiro in southern Bahia. Surveys of 54 mosquito breeding si...

  5. [Agrobacterium-mediated sunflower transformation (Helianthus annuus L.) in vitro and in Planta using strain of LBA4404 harboring binary vector pBi2E with dsRNA-suppressor proline dehydrogenase gene].

    PubMed

    Tishchenko, E N; Komisarenko, A G; Mikhal'skaia, S I; Sergeeva, L E; Adamenko, N I; Morgun, B V; Kochetov, A V

    2014-01-01

    To estimate the efficiency of proline dehydrogenase gene suppression towards increasing of sunflower (Helianthus annuus L.) tolerance level to water deficit and salinity, we employed strain LBA4404 harboring pBi2E with double-stranded RNA-suppressor, which were prepared on basis arabidopsis ProDH1 gene. The techniques of Agrobacterium-mediated transformation in vitro and in planta during fertilization sunflower have been proposed. There was shown the genotype-depended integration of T-DNA in sunflower genome. PCR-analysis showed that ProDH1 presents in genome of inbred lines transformed in planta, as well as in T1- and T2-generations. In trans-genic regenerants the essential accumulation of free L-proline during early stages of in vitro cultivation under normal conditions was shown. There was established the essential accumulation of free proline in transgenic regenerants during cultivation under lethal stress pressure (0.4 M mannitol and 2.0% sea water salts) and its decline upon the recovery period. These data are declared about effectiveness of suppression of sunflower ProDH and gene participation in processes connected with osmotolerance. PMID:25184200

  6. FUNDAMENTACIN Body mix es una modalidad del fitness que se ha creado con el fin de unificar

    E-print Network

    Escolano, Francisco

    BODY MIX FUNDAMENTACIÓN Body mix es una modalidad del fitness que se ha creado con el fin de unificar disciplinas como body pump, body balance, body combat y body attack. Vamos a definir brevemente en qué consiste cada una de las disciplinas: Body pump es un programa de entrenamiento que destaca por

  7. Todos los clientes no son iguales: una perspectiva financiera sobre servicio al cliente para los gerentes de mercadeo

    Microsoft Academic Search

    Harold Lee Koch

    1999-01-01

    Los gerentes colombianos estamos pensando en Servicio al Cliente como la próxima ventaja competitiva y una ayuda para contrarrestar los efectos de la recesión actual. Estamos cansados de buscar siempre en nuestra caja de herramientas de mercadeo y encontrar solamente el martillo del precio. Para diferenciamos de la competencia, hacerle al cliente menos sensible una diferencia en precio y encontrar

  8. El ALCA y el Consenso de Washington. Una mirada desde el comercio y la Teoría de las Relaciones Internacionales

    Microsoft Academic Search

    Gastón Acosta

    El trabajo intenta dar una aproximación a lo que ha sido el ALCA en términos de la política exterior comercial y económica de Estados Unidos hacia América Latina e intenta hacernos comprender cuales las consecuencias del Consenso de Washington para los países de este bloque desde una perspectiva comparada. El mismo comenzara con la siguiente hipótesis: ¿Puede la política exterior

  9. Una metodología para la resolución de problemas geométricos mediante el software educativo “elementos matemáticos” en la secundaria básica

    Microsoft Academic Search

    Liuska Martínez Noris; Osmany Carmenates Barrios

    2011-01-01

    En la Tercera Revolución Educacional de la Revolución Cubana se hace necesario introducir una nueva metodología que potencie la resolución de problemas geométricos mediante el uso de los Software Educativo. Se aplicó una metodología para el software “Elementos Matemáticos” de la Colección El Navegante de la Secundaria Básica. La misma tiene como propósito que sirva fundamentalmente de guía orientadora para

  10. Problemas de Topologia (Hoja 1) 1. Sea {(X, ), A} una familia arbitraria de espacios topologicos. Si para cada

    E-print Network

    Martín Peinador, Elena

    Problemas de Topolog´ia (Hoja 1) 25-II-08 1. Sea {(X, ), A} una familia arbitraria de espacios} una familia arbitraria de espacios topol´ogicos. y sea M X para cada A. En el producto A M se espacio producto. a) Si () X es un punto fijo, y H := (x) A X tal que x = , para casi todo , hallar la

  11. Assessing channel migration on decadal scales at Una inlet (Peruíbe, Brazil)

    NASA Astrophysics Data System (ADS)

    Chagas, F. M.; Ribeiro, J.; Italiani, D. M.; Siegle, E.; Coastal Dynamics Laboratory

    2013-05-01

    Inlets are often cited as ecologically important areas, been frequent subject of study in marine sciences and engineering. Controlling forces of these complex systems are the tidal variation, waves, sediment budget, river outflow and local morphology. Over years, migration can occur alongshore or crosshore as dynamic equilibrium occurs or one of dominant forces varies. The goal of this study is to analyze decadal migration of Una inlet over 1962 to 2006, by means of aerial photographs comparison and computational measurements of coastline variation, indicating physical controlling patterns that act over southern coastal area of Sao Paulo state. Una River (also locally named as Una do Prelado) is part of the main drainage basin of Juréia Ecological Reserve at Peruíbe, southern coast of Sao Paulo state, Brazil. It is 57 km long surrounding almost completely the Juréia Massif, reaching the sea through the Barra do Una inlet. Morphologically, Una beach at Barra do Una can be classified as intermediate or dissipative, composed mainly by very fine sand, well selected due to waves and microtidal currents. Available aerial photographs range from 1962 to 2006. These images have been analyzed and three mosaics were selected to represent the decadal evolution. Images were georeferenced through the ENVI software by RST method using nearest neighbor interpolation. Both inlet margins were digitalized considering the vegetation line as limit border of water-land. For migration calculations, a hand-picked longitudinal transect was established over the inlet, in which 10 parallel segments automatic calculate the horizontal variations over digitalized data at ArgGIS 9.3 system. Through this method, the main direction of channel migration (lateral movement southwest-northeast) was obtained. Upstream transects showed small variations of line margins, typically under 40 m, indicating a relative lateral stability area on the inlet. From transect #4 downstream direction, margin movement values increased greatly reaching -256 m at transect #6 and 602 m at transect #9. Migration values show significant lateral movement from 1962 to 1981 to the northeast, 132 m at the northern margin and 123 m at the southern margin. From 1981 to 2006 an overall southwestern migration has been observed, 51 m at the northern and 113 m at the southern margin, respectively. Net channel migration is to the northwest, with 81 m at the northern margin and 10 m at the southern margin. From 1962 to 2006, channel stabilization can be observed, resulting in a wider channel of the Una river mouth, which may have changed its exchanges with the adjacent ocean. Channel movements may be related to Furado Channel opening in 1958, a new stream that reduced in 10 km the river path to the mouth. This observed behavior complements the hypothesis cited by previous studies which pointed this upstream anthropogenic intervention as responsible for changes in downstream dominant forcings at Una inlet, modifying the original wave-tidal prism equilibrium state of the system.

  12. Maxima verosimilitud Tenemos una variable X discreta con funcion de masa

    E-print Network

    Fernández Gallardo, Pablo

    M´axima verosimilitud Tenemos una variable X discreta con funci´on de masa f (x; ), donde x A y #12;Llamamos funci´on de verosimilitud a la funci´on V V (; x1, . . . , xn) = n i=1 f (xi ln(f (xi ; )), la log-verosimilitud. Las funciones V y LV alcanzan el m´aximo en el mismo punto de

  13. El patrimoni educatiu, element d'una pedagògia cultural i ciutadana

    Microsoft Academic Search

    Isabel Carrillo; Eulàlia Collelldemont Pujadas; Pedro Luís Moreno

    2008-01-01

    El present article desenvolupa els eixos de pensament que s’estan elaborant des de diferents angles sobre el patrimoni educatiu. Una revisió sobre la importància de les aportacions de les investigacions a l’entorn del patrimoni educatiu permet assenyalar els punts d’inflexió dels museus pedagògics en l’actualitat. Entre aquests destaca la influència que els museus pedagògics, a través de les seves activitats

  14. Una alternativa Bayesiana a los contrastes de la bondad del ajuste

    Microsoft Academic Search

    F. J. Girón; C. R. Ortiz

    1985-01-01

    Resumen  Se considera el problema de hacer inferencias acerca del modelo beta simétrico, desde un punto de vista bayesiano. Los resultados\\u000a se aplican posteriormente al contraste de la bondad de ajuste en el caso de una hipótesis nula simple (sin parámetros marginales)\\u000a y en el caso de que la hipótesis nula conste de un número finito de modelos. Caso de que

  15. INCORPORA CION DE LAS TIC¥s EN EDUCACION SUPERIOR: SOCIALIZACION DE UNA EXPERIENCIA

    Microsoft Academic Search

    MARTHA LUCIA

    2009-01-01

    Se pretende describir y comprender los comportamientos y din·micas grupales de los estudiantes al incorporar actividades de una asignatura en un ambiente de aprendizaje virtual. Es un estudio cualitativo de caso y las categorÌas emergentes de an·lisis descriptivo son: la gestiÛn del tiempo de los estudiantes, la utilizaciÛn de recursos y herramientas, significados particulares hacia los entornos virtuales y caracterÌsticas

  16. Additional lichens and some lichenicolous fungi from the Una National Park (Bosnia and Herzegovina).

    PubMed

    Bilovitz, Peter O; Türk, Roman; Mayrhofer, Helmut

    2010-09-15

    A list of 62 taxa (61 species) of lichens and 7 species of lichenicolous fungi from the Una National Park in the north-western part of Bosnia and Herzegovina is presented. The lichens Caloplaca crenulatella, Dimerella pineti, Lecanora expallens, Lopadium disciforme, Placynthiella icmalea, Toninia athallina and the lichenicolous fungi Abrothallus bertianus, A. parmeliarum, Cornutispora lichenicola, Endococcus rugulosus, and Sphaerellothecium parmeliae are new to Bosnia and Herzegovina. PMID:22102780

  17. Primer Festival de Teatro Latinoamericano en Boston (marzo 2-5): Comienza una tradición

    E-print Network

    Ben-Ur, Lorraine Elena

    1977-04-01

    SPRING 1977 73 Primer Festival de Teatro Latinoamericano en Boston (marzo 2-5): Comienza una tradición LORRAINE ELENA BEN-UR Fue éste un acontecimiento de envergadura cultural y artística para Boston, ciudad multicultural cuya comunidad hispana... cuenta menos de quince años de existencia. Su importancia reside 1) en el hecho de que el público asistente se componía tanto de hispanos de la ciudad como norteamericanos de todo el área metropolitana, 2) en la calidad de los elencos teatrales que...

  18. Temporalidad e historia: hacia una reformulación del marco interpretativo del testimonio posdictatorial

    E-print Network

    Garibotto, Veró nica

    2010-01-01

    entre tomas interiores y exteriores está invertido. Si en Botín de guerra los interiores se convertían en una especie de refugio doméstico, en la serie las casas de las familias apropiadoras son reductos oscuros, casi macabros, sede ideal para las... exacto de las casas de las familias falsas: cálidos, cercanos, llenos de gente amable. Además de construir las oposiciones binarias sobre las que se sostiene la interpelación emo- cional de la serie—lo “macabro” y lo “puro”, lo “oscuro” y lo “claro...

  19. Additional lichens and some lichenicolous fungi from the Una National Park (Bosnia and Herzegovina)

    PubMed Central

    Bilovitz, Peter O.; Türk, Roman; Mayrhofer, Helmut

    2011-01-01

    A list of 62 taxa (61 species) of lichens and 7 species of lichenicolous fungi from the Una National Park in the north-western part of Bosnia and Herzegovina is presented. The lichens Caloplaca crenulatella, Dimerella pineti, Lecanora expallens, Lopadium disciforme, Placynthiella icmalea, Toninia athallina and the lichenicolous fungi Abrothallus bertianus, A. parmeliarum, Cornutispora lichenicola, Endococcus rugulosus, and Sphaerellothecium parmeliae are new to Bosnia and Herzegovina. PMID:22102780

  20. Problema 1. 6 puntos. Una empresa dispone de la red de la figura. Una VPN conecta la sede central de la izquierda con las dos sedes remotas de la

    E-print Network

    ) dibujar el intercambio de información entre cliente y servidor a partir del primer segmento de datos, hay una perdida. Dibujar la evolución de la ventana de transmisión, de congestión, anunciada y el

  1. Una aproximacin inicial al marco de utilizacin de los modelos en la empresa: los sistemas de informacin ................................. 1

    E-print Network

    Granada, Universidad de

    Contenido CAPÍTULO 1 Una aproximación inicial al marco de utilización de los modelos en la empresa: los sistemas de información ................................. 1 1.1 Consideraciones previas sobre el........................................................... 3 1.2 Los sistemas de información empresariales

  2. El espacio escénico y su influencia modificadora de la puesta en escena: Una experiencia del Teatro ICTUS de Chile

    E-print Network

    Genovese, Carlos

    1991-10-01

    FALL 1991 41 El espacio escénico y su influencia modificadora de la puesta en escena: Una experiencia del Teatro ICTUS de Chile Carlos Genovese En el devenir de nuestra experiencia teatral nos hemos formulado innumerables veces la siguiente... pregunta: ¿Hasta dónde el espacio escénico influye en la puesta en escena de una obra y es un factor determinante en la percepción que el espectador tiene de los contenidos formales y conceptuales que ésta encierra? Si entendemos por espacio escénico el...

  3. TAgentsP y PDP: propuestas para una plataforma de agentes en computacion ubicua

    Microsoft Academic Search

    Ma Celeste; Campo Vazquez; Carlos Garc; Florina Almenares; Mendoza Andres; Mar ´ in Lopez

    ser, presenta entornos saturados de dispositivos con capaci-dades de computaci'on y comunicaci'on que le proporcionan a los usuarios servicios ocultando la complejidad tecnol'ogi-ca subyacente. Nuestra l'inea de investigaci'on pretende po-tenciar el uso de la tecnolog'ia de agentes m'oviles en com-putaci'on ubicua para facilitar este objetivo. El trabajo que aqu'i presentamos se centra por un lado en proporcionar una plataforma de

  4. La Casa Verde de Mario Vargas Llosa: una imagen del hombre

    E-print Network

    Frick, Magdalena Capurro

    1976-01-01

    Christian La Casa Verde, una de las novelas mas importantes de Mario Vargas Llosa, es analizada en terminos de su imagen implfcita de la vida en America Latina ass corno en Peru, donde la novels se situs? La forms en que el esti lo y la tecnica... ~ ~ ~ ~ ~ ~ ~ e e 154 INTRODUCCI6N La obra de Mario Vargas Llosa, uno de los mas importantes escritores latinoamericanos contemporaneos, es representativa de las actuales tendencias en la lite ratura de America Latina En sus cuatro novelas y dos nouvelle...

  5. La guerra del fin del mundo: historia e invencion en una novela historica 

    E-print Network

    Loeffler, Lisa Bowe

    1984-01-01

    real al igual que los palos y las piedras". Tomando en cuenta el aspecto literario forzoso en la historiograffa, y la veracidad de una realidad interior que la ficcion historica pretende mostrar, ". . . history is no less a form of fiction than..., "Notes", 496-498). 18 La tercera faze empieza a finales del siglo diecinueve, y se trata de bandas independientes de los jefes locales que operaban por su propia cuenta. Eran bandidos que luchaban contra las volantes de la policia que los perseguia...

  6. Desarrollo de una cervecera en una región poco desarrollada a la luz de sus documentos contables: El Alcázar (1928–93). (Development of a brewery in an underdeveloped region in the light of its accounting documents: The Alcazar (1928–93))

    Microsoft Academic Search

    Alonso Moreno

    2011-01-01

    En este trabajo realizamos un análisis descriptivo de la historia de una empresa cervecera, desde una óptica eminentemente económico-contable, haciendo especial referencia a los cambios más significativos que han sufrido los documentos contables. El hecho de disponer de fuentes documentales durante un amplio período, 1928-93, permite una amplia visión de los cambios en la información suministrada por la compañía. Nuestro

  7. High-throughput in planta expression screening identifies a class II ethylene-responsive element binding factor-like protein that regulates plant cell death and non-host resistance.

    PubMed

    Nasir, Khairun Hisam Bin; Takahashi, Yoshihiro; Ito, Akiko; Saitoh, Hiromasa; Matsumura, Hideo; Kanzaki, Hiroyuki; Shimizu, Takeshi; Ito, Minako; Fujisawa, Shizuko; Sharma, Prakash C; Ohme-Takagi, Masaru; Kamoun, Sophien; Terauchi, Ryohei

    2005-08-01

    We performed high-throughput screening using the potato virus X (PVX) system to overexpress Nicotiana benthamiana genes in planta and identify positive regulators of cell death. This screening identified NbCD1, a novel class II ethylene-responsive element binding factor (ERF), as a potent inducer of the hypersensitive response (HR)-like cell death. NbCD1 expression was induced by treatments with INF1 elicitor and a non-host pathogen Pseudomonas cichorii. NbCD1 exhibited transcriptional repressor activity through its EAR motif, and this motif was necessary for NbCD1 to cause cell death. We identified 58 genes that displayed altered transcription following NbCD1 overexpression. NbCD1 overexpression downregulated the expression of HSR203, a negative regulator of hypersensitive death. Conditional expression of NbCD1 in Arabidopsis also caused cell death, indicating that NbCD1 downstream cascades are conserved in dicot plants. To further confirm the role of NbCD1 in defense, we used virus-induced gene silencing to demonstrate that NbCD1 is required for non-host resistance of N. benthamiana to the bacterial pathogen P. cichorii. Our data point to a model of transcriptional regulatory cascades. NbCD1 positively regulates cell death and contributes to non-host resistance, possibly by downregulating the expression of other defense response genes. PMID:16098104

  8. An updated model for nitrate uptake modelling in plants. I. Functional component: cross-combination of flow–force interpretation of nitrate uptake isotherms, and environmental and in planta regulation of nitrate influx

    PubMed Central

    Le Deunff, Erwan; Malagoli, Philippe

    2014-01-01

    Background and Aims In spite of major breakthroughs in the last three decades in the identification of root nitrate uptake transporters in plants and the associated regulation of nitrate transport activities, a simplified and operational modelling approach for nitrate uptake is still lacking. This is due mainly to the difficulty in linking the various regulations of nitrate transport that act at different levels of time and on different spatial scales. Methods A cross-combination of a Flow–Force approach applied to nitrate influx isotherms and experimentally determined environmental and in planta regulation is used to model nitrate in oilseed rape, Brassica napus. In contrast to ‘Enzyme–Substrate’ interpretations, a Flow–Force modelling approach considers the root as a single catalytic structure and does not infer hypothetical cellular processes among nitrate transporter activities across cellular layers in the mature roots. In addition, this approach accounts for the driving force on ion transport based on the gradient of electrochemical potential, which is more appropriate from a thermodynamic viewpoint. Key Results and Conclusions Use of a Flow–Force formalism on nitrate influx isotherms leads to the development of a new conceptual mechanistic basis to model more accurately N uptake by a winter oilseed rape crop under field conditions during the whole growth cycle. This forms the functional component of a proposed new structure–function mechanistic model of N uptake. PMID:24638820

  9. Análisis Media-semivarianza: Una Aplicación A Las Primas De Riesgo En El Mercado De Valores Español\\/Mean-semivariance Analysis: An Application To Risk Premiums In The Spanish Stock Market

    Microsoft Academic Search

    JOSÉ LUIS MIRALLES MARCELO; MARÍA DEL MAR MIRALLES QUIRÓS; JOSÉ LUIS. MIRALLES QUIRÓS

    2007-01-01

    La correcta medida del riesgo tiene una importancia fundamental en la valoración de activos. Sin embargo, una de las medidas más frecuentemente utilizadas, la varianza, ha recibido críticas debido a que establece una respuesta simétrica para movimientos positivos y negativos en las rentabilidades bursátiles, pudiendo ser una causa del rechazo del CAPM tradicional. El objetivo de este estudio consiste en

  10. In para totale...una cosa da panico...sulla lingua dei giovani in Italia (In para totale...una cosa da panico...The Language of Young People in Italy).

    ERIC Educational Resources Information Center

    Marcato, Carla

    1997-01-01

    Describes and analyzes the language of young people in Italy today. Particular focus is on the expressions using "para" (e.g., "in para totale" = to be very bored or worried) and the phrase "una cosa da panico" (something terrible or its opposite something wonderful). (CFM)

  11. 98 VHDL: LENGUAJE PARA S INTESIS Y MODELADO DE CIRCUITOS c En una funcion, y siempre con caracter opcional e informativo, existe la posibilidad

    E-print Network

    Valencia, Universidad de

    98 VHDL: LENGUAJE PARA S â?? INTESIS Y MODELADO DE CIRCUITOS c RA­MA En una funciâ??on, y siempre con carâ??acter opcional e informativo, existe la posibilidad de declararla como PURE o IMPURE. Un funciâ??on pura es una funciâ??on que devuelve siem­ pre el mismo valor para unos parâ??ametros de entrada dados. Una

  12. El precio medio del metro cuadrado de la vivienda libre: Una aproximación metodológica desde la perspectiva de la Geoestadística

    Microsoft Academic Search

    JOSÉ MARÍA MONTERO LORENZO

    2004-01-01

    El precio medio del metro cuadrado de la vivienda libre y su evolución en los últimos años es una de las cuestiones que más preocupan a la ciudadanía de nuestro país y, en consecuencia, a las autoridades políticas y económicas con competencias en materia de política de vivienda. La única referencia oficial sobre la cuestión es la publicación trimestral de

  13. La tasa de interés forward como predictora de la tasa de interés futura: una aproximación al caso chileno

    Microsoft Academic Search

    Víctor García

    Este artículo presenta algunos resultados acerca de la estructura en el tiempo de la tasa de interés en Chile. La primera sección está dedicada a presentar las distintas teorías sobre la relación entre la tasa de interés forward y la tasa futura, que determina una cierta forma de la estructura en el tiempo de la tasa de interés. La metodología

  14. El rol de la empresa responsable en el desarrollo económico. Una perspectiva económica en el espacio iberoamericano

    Microsoft Academic Search

    Enrique Sergio Pées-Boz

    Resumen. La responsabilidad social empresarial (RSE) integra el grupo de temas trascendentes que aún no ofrecen el suficiente caudal de información cuantitativa. Son muy escasos los estudios reali- zados desde la perspectiva de las ciencias económicas, a pesar de su relevancia para el logro de un desarrollo incluyente basado en una empresarialidad responsable. Consecuentemente, con nuestra reciente tesis doctoral hemos

  15. Una mirada de la última Reforma de la Educación Argentina desde las Políticas de Cooperación Educativa de los Organismos Internacionales

    Microsoft Academic Search

    María Blanca Pesado Riccardi

    El presente trabajo es una investigación cualitativa que indaga sobre la relación entre la agenda educativa de los organismos internacionales y la última reforma educativa en nuestro país mediante la técnica de estudio de caso. Con ese criterio, se expone un análisis del cambio educativo argentino identificando causas y consecuencias del fenómeno y advirtiendo el rol que adquirieron los principales

  16. Facebook i tuits en bon valenci: una iniciativa nascuda al si de la UA Alacant, 6 de juny de 2012

    E-print Network

    Escolano, Francisco

    Facebook i tuits en bon valencià: una iniciativa nascuda al si de la UA Alacant, 6 de juny de 2012 universitària de la Universitat d'Alacant en Facebook i Twitter. En Bon Valencià ens proporciona cada dia, directament en el nostre compte de Twitter o Facebook, xicotetes dosis de correcció lingüística, en el format

  17. La Influencia de Jugadores de América Latina y España en El NBA de Una Perspectiva Mercadeo y de Negocios

    Microsoft Academic Search

    Michael Ethan Lee

    2012-01-01

    La influencia española y latinoamericana es muy evidente en el NBA. Hay muchos jugadores de América Latina y España que cambiaron la liga. El éxito de jugadores de América Latina y España ha aumentado el número de aficionados latinos y españoles en todo el mundo, y por eso el NBA está cambiando de una perspectiva de mercadeo y de negocios

  18. DE PADRES Y FAMILIAS La Oficina de Programas de Padres y Familias busca crear una buena relacin

    E-print Network

    Smith, Jerome A.

    PROGRAMAS DE PADRES Y FAMILIAS La Oficina de Programas de Padres y Familias busca crear una buena relación con los padres de familia y familiares de nuestros estudiantes de UC San Diego. Vemos la relación ayudaremos a navegar la Universidad, asistir a usted y a su estudiante, ¡y hacerlo sentir como parte de la

  19. La muerte del letrado Este trabajo estudia un fenmeno de suma importancia: el paso de una monarqua

    E-print Network

    Paris-Sud XI, Université de

    legitimidad al sistema de gobierno permaneció aparentemente intacta en sus fundamentos: evolucionó progresiva que los autores no disponían entonces de una visión global adecuada del sistema de gobierno de la dignidad: la calidad de ministro se tenía, en las informaciones de limpieza de sangre del siglo XVIII, por

  20. Desarrollo de una metodología de implementación de los conceptos de TOC (Teoría de restricciones), para empresas colombianas

    Microsoft Academic Search

    José Arturo González; Katherine Ortegón; Leonardo Rivera

    2003-01-01

    La Teoría de Restricciones (TOC) es un sistema de pensamiento gerencial que ha sido aplicado con éxito en multitud de empresas en el mundo. Sin embargo, no existe una documentación adecuada de su implementación en Pequeñas y Medianas Empresas (PyMEs) en Colombia, por lo que no hay un protocolo de implementación que guíe al empresario en dicho proceso. Al realizar

  1. ¿La Cerdanya, una ciudad al final del milenio? Metamorfosis del espacio, la identidad y el paisaje cerdà

    Microsoft Academic Search

    Pep Vivas i Elías

    2004-01-01

    Esta tesis está planteada en forma de viajes y, por tanto, está repleta de recorridos a través de la experiencia de uno mismo como ciudadano\\/a, de trayectos particulares sobre ámbitos de saber concretos, de etapas particulares vividas al traspasar los diferentes espacios cotidianos, etc. Todo ello se convierte en los fundamentos que muestran una visión particular (de las muchas visiones

  2. Estimación de una función de demanda de bienes de consumo duradero en América Latina 1890-1913

    Microsoft Academic Search

    Carolina Román Ramos

    En este trabajo se realiza una estimación de la demanda por importación de bienes de consumo duradero en América Latina en el período 1890-1913. Por un lado, se presenta la construcción del consumo de bienes duraderos a través de las estadísticas de importaciones para seis países de la región: Argentina, Brasil, Chile, Cuba, México y Uruguay. En segundo lugar, partiendo

  3. El parque tecnolgico de Pasto, Colombia, crear una oficina de transferencia basada en la de la Universidad de Alicante

    E-print Network

    Escolano, Francisco

    El parque tecnológico de Pasto, Colombia, creará una oficina de transferencia basada en la de la Parquesoft, el Parque Tecnológico del Software de Pasto, Colombia. El objetivo de la visita, que se Pasto (Colombia), es un centro de desarrollo tecnológico ideado para articularse como Cluster de Arte

  4. RESUMEN. Este trabajo representa una revisin y ac-tualizacin de la avifauna del estado de Sonora, Mxi-

    E-print Network

    Montana, University of

    385 RESUMEN. Este trabajo representa una revisión y ac- tualización de la avifauna del estado de information from 1849 to 2008. The AVIFAUNA J. FERNANDO VILLASEÑOR-GÓMEZ,1 OSVEL HINOJOSA-HUERTA,2 EDUARDO GÓMEZ-LIMÓN,3 DAVID KRUEPER4 Y AARON D. FLESCH5 avifauna of Sonora includes 556 species belonging to 73

  5. ste artículo se referirá exclusivamente al estudio del caso venezolano y no hará referencia a comparaciones internacionales. Se concretará en una descripción del fenómeno y a una argumentación acerca de los factores asociados a la exclusión escolar, según informaciones y observaciones realizadas en estudios y experiencias de campo en escuelas de Venezuela. En una primera parte se describirá la proporción de desertores, según el grado en el que ocurra el fenómeno y sus consecuencias cuantitativas, según datos oficiales de instancias especializadas

    Microsoft Academic Search

    Mariano Herrera

    1 . Una segunda parte analizará la relación entre educación y pobreza también desde una perspectiva cuantitativa y con enfoque económico. En la tercera parte se abordan los factores educativos asociados al fracaso escolar, es decir no sólo a la deserción sino también a la repitencia, entendiendo que esta es un factor de riesgo de deserción, dependiente del sistema educativo.

  6. In Planta Processing and Glycosylation of a Nematode CLAVATA3/ENDOSPERM SURROUNDING REGION-Like Effector and Its Interaction with a Host CLAVATA2-Like Receptor to Promote Parasitism1[OPEN

    PubMed Central

    Chen, Shiyan; Lang, Ping; Chronis, Demosthenis; Zhang, Sheng; De Jong, Walter S.; Mitchum, Melissa G.

    2015-01-01

    Like other biotrophic plant pathogens, plant-parasitic nematodes secrete effector proteins into host cells to facilitate infection. Effector proteins that mimic plant CLAVATA3/ENDOSPERM SURROUNDING REGION-related (CLE) proteins have been identified in several cyst nematodes, including the potato cyst nematode (PCN); however, the mechanistic details of this cross-kingdom mimicry are poorly understood. Plant CLEs are posttranslationally modified and proteolytically processed to function as bioactive ligands critical to various aspects of plant development. Using ectopic expression coupled with nanoliquid chromatography-tandem mass spectrometry analysis, we show that the in planta mature form of proGrCLE1, a multidomain CLE effector secreted by PCN during infection, is a 12-amino acid arabinosylated glycopeptide (named GrCLE1-1Hyp4,7g) with striking structural similarity to mature plant CLE peptides. This glycopeptide is more resistant to hydrolytic degradation and binds with higher affinity to a CLAVATA2-like receptor (StCLV2) from potato (Solanum tuberosum) than its nonglycosylated forms. We further show that StCLV2 is highly up-regulated at nematode infection sites and that transgenic potatoes with reduced StCLV2 expression are less susceptible to PCN infection, indicating that interference of the CLV2-mediated signaling pathway confers nematode resistance in crop plants. These results strongly suggest that phytonematodes have evolved to utilize host cellular posttranslational modification and processing machinery for the activation of CLE effectors following secretion into plant cells and highlight the significance of arabinosylation in regulating nematode CLE effector activity. Our finding also provides evidence that multidomain CLEs are modified and processed similarly to single-domain CLEs, adding new insight into CLE maturation in plants. PMID:25416475

  7. El laboratori informàtic a l’aula. L’Interactive Physics, fent servir el canó de projecció, una bona eina per ensenyar física

    Microsoft Academic Search

    Juli Tena i Frasno

    2009-01-01

    Les simulacions són un recurs informàtic que ajuda en la tasca docent, però ens trobem amb la dificultat que suposa aplicar una tecnologia amb la que no estem familiaritzats. El que proposo és una metodologia per utilitzar les simulacions IP a l’aula convencional, de manera que no impliqui l’ús d’altres espais.

  8. Las Hidroeléctricas deben contribuir para un desarrollo territorial sostenible! Una revisión de perspectivas, contradicciones y opciones urgentes para territorios rurales en Guatemala

    Microsoft Academic Search

    Dagny Skarwan

    2011-01-01

    Las hidroeléctricas se convierten en una potencial alternativa energética frente a los combustibles fósiles. Abundan las contradicciones en distintos planos que se generan a partir de un sistema excluyente y visiones centralistas del sector energético en Guatemala. Enfocarlas será una precondición para proponer cambios a favor del desarrollo de los territorios donde se generan los recursos hídricos del país, pero

  9. Desarrollo sustentable participativo en una cuenca hidrográfica de montaña. Bosque Modelo Jujuy, Provincia de Jujuy, Noroeste Argentino

    Microsoft Academic Search

    Virginia Outon; Ralf Schillinger; Pedro Williams; Rolando Braun-Wilke; Guillermina Díaz

    2009-01-01

    Se implementa, desde 1999, un programa de gestión de recursos naturales, tomando como unidad de manejo la cuenca del río Perico - Manantiales -150.000 ha, tributaria del río Grande-Lavayén-, que comprende biomas de Yungas, Altoandino y Chaco Serrano . Es una cuenca montañosa co n ríos torrenciales (altitudes 550-5.600 msnm) propensa a erosión hídrica, eólica y criogénica por causas naturales

  10. EFFETTO DELL'ORIENTAMENTO DELLE FIBRE SULLA RESISTENZA A FATICA DI UNA POLIAMMIDE 6 RINFORZATA CON FIBRE DI VETRO

    Microsoft Academic Search

    A. Bernasconi; P. Davoli; A. Basile; A. Filippi

    Sommario In questo lavoro vengono presentati i risultati dello studio dell'effetto dell'orientamento delle fibre sulla resistenza a fatica di una poliammide 6. sono state eseguite prove di fatica su provini estratti da lastre ottenute per stampaggio ad iniezione. I provini sino stati ritagliati con diversi orientamenti rispetto all'asse della lastra, in modo da ottenere differenti orientamenti delle fibre di rinforzo

  11. L’ensenyament de la Toxicologia a Farmàcia: Els seminaris com una eina per a l’avaluació continuada

    Microsoft Academic Search

    Jesús Gómez-Catalán; Marta Barenys Espadaler; Elisabet Teixidó Condomines; María Esther Piqué Benages; Nuria Boix Sabria; Joan M. Llobet Mallafré; Miguel Rodamilans Pérez

    2010-01-01

    Amb la finalitat d’adaptar-nos a l’EEES, vàrem desenvolupar una eina que ens permetés realitzar un procés d’avaluació continua de l’assignatura troncal de Toxicologia. A continuació, presentem els resultats d’aquest model en el que utilitzem els seminaris com a elements integradors del procés d’avaluació, així com la seva estructura, desenvolupament i puntuació. \\u000aAquests seminaris es van avaluar amb un màxim del

  12. DIRECCIN DE PLANTA FSICA VICERRECTORADO ADMINISTRATIVO

    E-print Network

    Vásquez, Carlos

    . CON BALASTRO ELECTRONICO MULTIVOLTAJE CON REJILLA SEMIESPECULAR Y SOCATES DE SEGURIDAD. (A ser FLUORESCENTE SUPERFICIAL (120X60cm) CON TUBO FLUORESCENTE 3X32W TIPO T8. CON BALASTRO ELECTRONICO MULTIVOLTAJE

  13. Bancos de Genes de Plantas: Seguridad Alimenticia

    NSDL National Science Digital Library

    Geoffrey C. Hawtin and Jeremy Cherfas (International Plant Genetic Resources Institute; )

    2003-04-01

    The issue-focused, peer-reviewed article shows how genebanks are like an insurance policy for the future of agriculture, for they: conserve the diversity of plant species, offer resources for breeding of hardier crop varieties, provide food solutions in times of disaster, and safeguard food supplies for future generations.

  14. Simple quantification of in planta fungal biomass.

    PubMed

    Ayliffe, Michael; Periyannan, Sambasivam K; Feechan, Angela; Dry, Ian; Schumann, Ulrike; Lagudah, Evans; Pryor, Anthony

    2014-01-01

    An accurate assessment of the disease resistance status of plants to fungal pathogens is an essential requirement for the development of resistant crop plants. Many disease resistance phenotypes are partial rather than obvious immunity and are frequently scored using subjective qualitative estimates of pathogen development or plant disease symptoms. Here we report a method for the accurate comparison of total fungal biomass in plant tissues. This method, called the WAC assay, is based upon the specific binding of the plant lectin wheat germ agglutinin to fungal chitin. The assay is simple, high-throughput, and sensitive enough to discriminate between single Puccinia graminis f.sp tritici infection sites on a wheat leaf segment. It greatly lends itself to replication as large volumes of tissue can be pooled from independent experiments and assayed to provide truly representative quantification, or, alternatively, fungal growth on a single, small leaf segment can be quantified. In addition, as the assay is based upon a microscopic technique, pathogen infection sites can also be examined at high magnification prior to quantification if desired and average infection site areas are determined. Previously, we have demonstrated the application of the WAC assay for quantifying the growth of several different pathogen species in both glasshouse grown material and large-scale field plots. Details of this method are provided within. PMID:24643560

  15. Exposicin de cazatormentas espaoles,para disfrutar de los fenmenos meteorolgicos a travs de una gran coleccin de fotos y vdeos de socios de ACAmet y cazatormentas.net

    E-print Network

    Escolano, Francisco

    de una gran colección de fotos y vídeos de socios de ACAmet y cazatormentas.net La exposición está pantallas, una con 60 fotografías distintas y otra de una serie de vídeos de fotos time-lapse para ver la evolución de nubes, cascadas de nubes y creación de tormentas. La selección de las fotos de la muestra se ha

  16. Memorias del Segundo Simposio Internacional Sobre Polticas, Planificacin y Economa de los Programas de Proteccin Contra Incendios Forestales: Una Visin Global Memorias del Segundo Simposio Internacional Sobre Polticas, Planificacin y Economa de los Progra

    E-print Network

    Standiford, Richard B.

    , socials, financieras y medioambientales han dado lugar a una amplia gama de sistemas de organización de la históricas, políticas, sociales, medioambientales y financieras han dado lugar a una amplia gama de sistemas dar una visión de este problema que pueda ser de utilidad cuando se estén adoptando estas decisiones

  17. Las propiedades de las estrellas extrañas en el marco de una nueva ecuación de estado para la materia extraña

    NASA Astrophysics Data System (ADS)

    Lugones, G.; Benvenuto, O. G.

    Se estudian las propiedades generales de las estrellas constituídas por materia extraña (ME) en el marco de una nueva ecuación de estado (EOS) en la que consideramos la masa de los quarks como dependiente del número medio de bariones por unidad de volumen. Se asume esta dependencia de forma que los quarks sean livianos (pesados) a densidades altas (bajas). En esta aproximación, la EOS de la ME es similar a la predicha por el modelo de la Bolsa del MIT, pero es significativamente mas dura a bajas densidades. Esta propiedad modifica las propiedades de las estrellas extrañas en forma notable. Encontramos que, con esta nueva EOS, los objetos pueden ser más masivos que en el caso de la EOS de la bolsa del MIT y que, además, pueden presentar mayores redshifts gravitatorios en hasta un 10%. En el caso de las oscilaciones radiales de estos objetos, calculamos la relación período vs. redshift gravitacional y encontramos una expresión analítica simple para el caso de las oscilaciones de objetos de baja masa. Encontramos que, aún con hipótesis muy diferentes en cuanto a la ecuación de estado de la materia extraña, las propiedades generales de estos objetos no se ve afectada en forma fundamental, y, por lo tanto, no deberían ser muy diferentes de las aquí expuestas.

  18. L’arteriopatia perifèrica i la seva relació amb la qualitat de vida en una població amb factors de risc cardiovascular

    Microsoft Academic Search

    Bobé Armant Francesc; Buil Arasanz Maria Eugenia; Trubat Muñoz Griselda; Allué Buil Ana Isabel; Morro Grau Amàlia; Bladé Creixenti Jordi

    2011-01-01

    Introducció. Els pacients amb claudicació intermitent presenten elevada incidència de malaltia coronària, però freqüentment són asimptomàtics Objectiu. Determinar la prevalença d’Arteriopatia Perifèrica Silent en una població amb Factors de Risc Cardiovascular (FRCV) i la forma de presentació (insuficiència o calcificació arterial). Analitzar repercussió de la malaltia arterial perifèrica sobre la percepció de qualitat de vida relacionada amb la salut (CVRS)

  19. Una tecnica per misurare il recupero della coscienza al letto del paziente Uno studio dell'Universit degli Studi di Milano e il Coma Science Group di Liegi,

    E-print Network

    De Cindio, Fiorella

    'Università degli Studi di Milano e il Coma Science Group di Liegi, pubblicato su Brain, impiega una combinazione Scienze Cliniche "Luigi Sacco" dell'Università degli Studi di Milano e il Coma Science Group di Steven cerebrolesi che hanno mostrato un'evoluzione dal coma verso altri stati clinici. "La TMS/EEG" commenta il Dott

  20. EVALUACIÓN DEL IMPACTO DE UNA PLATAFORMA PARA LA GESTIÓN DEL APRENDIZAJE UTILIZADA EN CURSOS PRESENCIALES EN EL INSTITUTO TECNOLÓGICO DE SONORA

    Microsoft Academic Search

    Omar Cuevas-Salazar; Ciudad Obregón; Ramona Imelda García-López; Roberto Cruz-Medina

    2008-01-01

    OMAR CUEVAS-SALAZAR \\/ RAMONA IMELDA GARCÍA-LÓPEZ \\/ ISIDRO ROBERTO CRUZ-MEDINA Resumen: La finalidad de esta investigación es evaluar el efecto de una plataforma tecnológica en el aprovechamiento escolar de los estudiantes y la percepción de los distintos actores involucrados con respecto a su utilidad. La evaluación de esta plataforma, denominada Sistema de Apoyo a la Educación con Tecnologías de Internet

  1. Test. (5 punts) Les preguntes del test poden ser multiresposta (MR) o de resposta nica (RU). Una pregunta MR val 0,5 punts si la

    E-print Network

    Politècnica de Catalunya, Universitat

    Test. (5 punts) Les preguntes del test poden ser multiresposta (MR) o de resposta única (RU). Una porta la capçalera TCP. Un client TCP pot passar per l'estat listen. MR. Digues quines de les següents de TCP (wnd) pot ser major que la finestra advertida (awnd) que portava l'última confirmació que ha

  2. Enseñar y aprender en la Sociedad del Conocimiento: el trabajo independiente y la labor del tutor una alternativa para su concreción

    Microsoft Academic Search

    Eldis Román Cao; José Ignacio Herrera Rodríguez

    2009-01-01

    El presente artículo expone ideas relacionadas con necesidades que apremian en los contextos educativos contemporáneos relacionados con las formas de enseñar y aprender. A partir de la revisión de un número considerable de bibliografía se presentan un conjunto de consideraciones teóricas que se sustentan en las vivencias y experiencias del autor como profesor-tutor desde la práctica pedagógica, se realiza una

  3. Cálculo del ranking acumulado para la encuesta de expectativas de inflación y tasa de cambio nominal, a través de una prueba no paramétrica

    Microsoft Academic Search

    Wilmer O. Martínez R; Manuel D. Hernández

    2012-01-01

    En este documento se presenta una metodología para el cálculo del Ranking acumulado de las entidades financieras que participan en la encuesta mensual de expectativas de inflación y tasa de cambio nominal (TRM), realizada por el Banco de la República. La metodología se basa en la prueba estadística no paramétrica propuesta por Jonckheere (1954), que consiste en un sistema de

  4. La responsabilidad Social Empresarial (RSE) en Europa: la apuesta por un nuevo modelo de empresa. Una revisión crítica desde la ética empresarial

    Microsoft Academic Search

    Elsa González Esteban; Vicente Domingo García Marzá

    2006-01-01

    El presente artículo tiene un doble objetivo. Por una parte, poner de manifiesto el desarrollo del discurso europeo respecto a la necesidad de asunción de valores y principios sociales y medioambientales en la gestión y dirección de las empresas con la finalidad de alcanzar altas cotas de competitividad y de cohesión social. Por otra parte, ofrecer un modelo de Responsabilidad

  5. DIMENSIONALIDAD DE LA RESPONSABILIDAD SOCIAL EMPRESARIAL PERCIBIDA Y SUS EFECTOS SOBRE LA IMAGEN Y LA REPUTACIÓN: UNA APROXIMACIÓN DESDE EL MODELO DE

    Microsoft Academic Search

    ALEJANDRO ALVARADO HERRERA; MARÍA WALESSKA SCHLESINGER DÍAZ

    2008-01-01

    La literatura de marketing sugiere que las acciones y programas de responsabilidad social empresarial podrían mejorar la imagen y la reputación de las empresas ante los ojos de sus consumidores y ser una importante fuente de ventaja competitiva para las mismas. Sin embargo, poco se sabe acerca las percepciones de estos stakeholders al respecto y sobre sus efectos en dichas

  6. Olimpiadas de Ciencias Las Olimpiadas de Ciencias son unas competiciones de carcter intelectual dirigidas al alumnado de 1 y 2 de

    E-print Network

    Escolano, Francisco

    Olimpiadas de Ciencias Las Olimpiadas de Ciencias son unas competiciones de carácter intelectual, e internacional. Sus objetivos son: Estimular el estudio de las ciencias entre los/las jóvenes y relación entre los centros de educación secundaria y la Universidad. La Facultad de Ciencias organiza las

  7. Seminario "Divulgacin de conocimientos en ciencias de la nutricin" DESTINADO A: Se trata de una actividad enfocada a todos los estudiantes del Grado

    E-print Network

    Escolano, Francisco

    Seminario "Divulgación de conocimientos en ciencias de la nutrición" DESTINADO A: Se trata de una a cualquier estudiante y profesional de ciencias de la salud, hasta completar aforo. FECHA y HORA: martes 17 de diciembre de 2013, de 16:00 a 18:00. LUGAR: Salón de grados de la Facultad de Ciencias de la Salud

  8. Son decenas y estn por toda Amrica Latina. Tienen en comn su amor por la ciencia y una curiosidad del tamao

    E-print Network

    Barbosa, Marcia C. B.

    Son decenas y están por toda América Latina. Tienen en común su amor por la ciencia y una la Red Inter-Americana de Academias de Ciencias (IANAS, por sus siglas en inglés), la Unesco, la ONU mujeres que lideran la ciencia en América Latina Margarita Rodríguez BBC Mundo Miércoles, 16 de octubre de

  9. Diálogo Regional de Política de América Latina y el Caribe: Retos y oportunidades en adaptación al cambio climático en materia de agua: Elementos para una agenda regional

    Microsoft Academic Search

    Fernando Miralles-Wilhelm; Enrique Aguilar; Juan Antonio Arrese Luco; Víctor Arroyo; Isabel Badillo Ibarra; Maureen Ballestero Vargas; Eugenio Barrios Ordoñez; Irene Burgues; Adán Carro de la Fuente; Marie-Violaine Chabrel; Sergio Lozano Torres; Rocío Córdoba; Víctor Díaz; Mario Cordero Vejar; Estrellita Mireya Fuentes Nava; Roberto Galan; María Concepción García Gómez; José Luis Genta; Guillermo Gutiérrez Gómez; Josué Isaac Hernández Díaz; José María Hinojosa Aguirre; Carlos Alberto Hurtado Aguilar; Matthias Krause; Rosalva Landa; Flavia Loures; Mario López Pérez; Víctor Magaña; Mario Manzano Camarillo; Julia Martínez; José Luis Martínez Ruiz; Carlos Maturano Rodríguez; Grisell Medina Laguna; Jorge Meza; Jorge Mora Portuguez; Erick Mota; Daniel Murillo Licea; Bruno Pagnoccheschi; Claudia Rendón; Luis Rendón Pimentel; Patricia Resendiz; Jorge Alberto Reyes Gaytán; Andrés Rodríguez; Katia Karina Rodríguez Ramos; Diana Rojas; Pamela Alejandra Rojas Hernández; Eddie Rosazza Asin; Juan Carlos Sánchez; Claudia Olivia Sánchez Pérez; Yerania Sánchez Ramos; Nathalie Seguin; Diana Siller; Denise Soares; Sergio Soto; Marco Antonio Velázquez Holguín; Simone Vendruscolo; Ricardo Alaín Villón Bracamonte; Javier Zuleta

    2010-01-01

    El presente documento representa un esfuerzo coordinado entre varias instituciones y organizaciones de la región de América Latina y el Caribe por plasmar los resultados de una reflexión conjunta sobre el tema de la adaptación al cambio climático en la comunidad hídrica y en el marco de un Diálogo Regional de Política. El propósito principal de este Diálogo es el

  10. Aplicacin del uso de valoraciones hesitant lingsticas para una consultora de Rosana Montes1, Ana Ma Sanchez1, Pedro Villar1, Francisco Herrera2

    E-print Network

    Granada, Universidad de

    de decisión lingüística. 1 INTRODUCCIÓN La toma de decisiones es una actividad diaria, que va des- de complejidad im- plícita en un problema, salvo cuando intentamos realizar la toma de decisiones con modelos problemas de Toma de Decisiones la posi- bilidad de proporcionar valoraciones poco precisas [9]. En este

  11. 1 'Advances in Genetic Programming III, Research and Educational use only' Lee Spector, W. B. Langdon, Una-May O'Reilly, Peter Angeline

    E-print Network

    Fernandez, Thomas

    1 'Advances in Genetic Programming III, Research and Educational use only' Lee Spector, W. B. Langdon, Una-May O'Reilly, Peter Angeline Welcome to the third volume of Advances in Genetic Programming series. The Genetic Programming (GP) field has matured considerably since the first two volumes were pro

  12. Applying New Technologies: ANT Automation es una empresa de Automatizacin Industrial, con operaciones en USA, Argentina y Espaa. La empresa est conformada por un slido grupo de

    E-print Network

    Maguitman, Ana Gabriela

    Applying New Technologies: ANT Automation es una empresa de Automatización Industrial, con más de 12 años de experiencia en Software/Automatización Industrial y por nuevos jóvenes talentos con el desarrollo de productos de software industrial: OPC, SCADA, MES, VISION ARTIFICIAL, MODELOS

  13. MODELO MATEMÁTICO PARA EL ORDENAMIENTO DE TAREAS EN UN FLOW-SHOP HÍBRIDO A DOS ETAPAS CON UNA RESTRICCIÓN DE BLOQUEO PARTICULAR

    Microsoft Academic Search

    Sergio Martínez de la Piedra

    2006-01-01

    Este artículo presenta un modelo de programación lineal en números enteros mixtos, el cual, puede ser utilizado para resolver el problema de minimización del tiempo total de paso de un grupo de tareas por un sistema de producción tipo Flow-Shop híbrido a dos etapas sin capacidad de almacenamiento entre ellas y con una nueva restricción de bloqueo, la cual, ha

  14. Estudio de los NIH señala una relación de la tomografía computarizada (TC) en la infancia con leucemia y con cáncer de cerebro más tarde

    Cancer.gov

    Niños y adultos jóvenes a quienes se les hicieron exploraciones muchas veces con tomografía computarizada (TC), una herramienta de diagnóstico de uso frecuente, tienen un riesgo ligeramente mayor de padecer leucemia y tumores cerebrales en la década posterior a su primera tomografía.

  15. UNA PROPUESTA DE PROYECTO DE INNOVACIÓN EDUCATIVA: SEMINARIOS Y TUTORÍAS; DOS ESTRATEGIAS DIDÁCTICAS PARA LA NUEVA ENSEÑANZA DE LAS DISCIPLINAS IUSHISTÓRICAS

    Microsoft Academic Search

    Belén Malavé Osuna

    2010-01-01

    En las páginas que siguen se describe una propuesta de innovación educativa que consiste en ensayar dos estrategias didácticas de máxima importancia en el contexto del nuevo modelo educativo impulsado desde el EEES, los seminarios y las tutorías, en dos asignaturas ligadas entre sí por su carácter histórico: Historia del Derecho y Derecho Romano. La técnica del seminario será experimentada

  16. Una societ del Gruppo ABB An ABB Group company Sede Legale/ Registered Office: Capitale Sociale/Share Capital: 107.000.000.-i.v./fully paid up

    E-print Network

    Segatti, Antonio

    ABB S.p.A. Una società del Gruppo ABB An ABB Group company Sede Legale/ Registered Office: Capitale/VAT: IT11988960156 Codice Fiscale/Fiscal Code: 00736410150 Direzione e Uffici/ Headquarters and Offices, Process Automation, Discrete Automation and Motion. Ricerchiamo, per la divisione Power Systems che si

  17. Una cierta rareza

    E-print Network

    Carballido, Emilio

    1992-10-01

    hipnosis deslumbrante de los Goyas negros, o, de nuevo, de J.C. Orozco. De todo ese concurso de la Corporación, entre tantas comparaciones, podían advertirse también en Viviescas factores gratuitos: talento, que se tiene de balde pero que cuesta mucha...

  18. ALGEBRA I. HOJA 5 1) Sea G una grupo y sea N un subgrupo normal de G. Sea x un elemento de torsion de G. Demostrad que el

    E-print Network

    Otero Domínguez, Margarita

    ´ALGEBRA I. HOJA 5 1) Sea G una grupo y sea N un subgrupo normal de G. Sea x un elemento de torsi que la aplicaci´on f : A - A definida por f(a) = ak es un isomorfismo. 5) Sea N un subgrupo normal de implica que xN es N. 6) Sea G un grupo abeliano con n elementos y p un entero primo con n. Demostrad que

  19. TOPOLOGIA 1 e Topologia per Fisica 22/6/2009 1. Esercizio. Se X `e spazio topologico e Y `e un insieme, una funzione f : X Y

    E-print Network

    Marconi, Umberto

    TOPOLOGIA 1 e Topologia per Fisica ­ 22/6/2009 1. Esercizio. Se X `e spazio topologico e Y `e un insieme, una funzione f : X Y `e detta localmente costante se per ogni p X esiste un intorno U di p in X tale che la restrizione f|U `e costante. Dimostrare che se X `e connesso, allora ogni f : X Y che sia

  20. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Paris-Sud XI, Université de

    NUEVA GOBERNABILIDAD POPULAR TANTO A NIVEL NACIONAL COMO LOCAL Elsa Cedeño1 Resumen La meta de este grandes instituciones y con una profunda reforma de la Constitución. Así, la gobernabilidad popular consta

  1. Memorias del Segundo Simposio Internacional Sobre Polticas, Planificacin y Economa de los Programas de Proteccin Contra Incendios Forestales: Una Visin Global Memorias del Segundo Simposio Internacional Sobre Polticas, Planificacin y Economa de los Progra

    E-print Network

    Standiford, Richard B.

    una teoría contable del incendio que identifique y valore el daño económico total que se espera evitar con origen en el coste de la lucha contra incendios. La teoría contable que se sigue responde a los

  2. 5. Espais afins euclidians 5.1 Considerem en un pla afi euclidi`a una refer`encia {O; e1, e2} on e1e2 = 60

    E-print Network

    Casacuberta, Carles

    5. Espais afins euclidians 5.1 Considerem en un pla af´i euclidi`a una refer`encia {O; e1, e2} on e. Calculeu les coordenades de la projecci´o ortogonal del punt (1, -2, 3) sobre el pla x - y + 2z = 0 entre ells. Trobeu la dist`ancia del punt al pla en tots dos casos. 5.3 Sigui {O; e1, e2, e3} una refer

  3. MÉTODO SIMPLE PARA LA DETECCIÓN MÚLTIPLE DE VIROIDES DE CÍTRICOS A SIMPLE METHOD FOR THE MULTIPLE DETECTION OF CITRUS VIROIDS

    Microsoft Academic Search

    Isidro H. Almeyda-León; Mario A. Rocha-Peña; M. Magdalena Iracheta-Cárdenas; Fermín Orona-Castro; Craig J. Kahlke

    La detección de viroides mediante RT-PCR en México es de uso común en los laboratorios de acreditación para determinar la calidad sanitaria de plantas de cítricos, cuando se van a utilizar árboles madre para propagar plantas de vivero. En el presente trabajo se describe una metodología simple para la detección múltiple de los viroides Citrus exocortis viroid (CEVd), CVd II,

  4. 4-H Activities (Spanish) 

    E-print Network

    Howard, Jeff W.

    2005-05-10

    ?a, una tienda de art?culos para jardines, un corral de ganado, una lecher?a o una planta el?ctrica. Los miembros de 4-H pueden aprender lecciones valiosas al visitar la corte del condado, las oficinas de un peri?dico, bancos, hoteles, los...

  5. Elucidation and in planta reconstitution of the parthenolide biosynthetic pathway.

    PubMed

    Liu, Qing; Manzano, David; Tani?, Nikola; Pesic, Milica; Bankovic, Jasna; Pateraki, Irini; Ricard, Lea; Ferrer, Albert; de Vos, Ric; van de Krol, Sander; Bouwmeester, Harro

    2014-05-01

    Parthenolide, the main bioactive compound of the medicinal plant feverfew (Tanacetum parthenium), is a promising anti-cancer drug. However, the biosynthetic pathway of parthenolide has not been elucidated yet. Here we report on the isolation and characterization of all the genes from feverfew that are required for the biosynthesis of parthenolide, using a combination of 454 sequencing of a feverfew glandular trichome cDNA library, co-expression analysis and metabolomics. When parthenolide biosynthesis was reconstituted by transient co-expression of all pathway genes in Nicotiana benthamiana, up to 1.4?gg(-1) parthenolide was produced, mostly present as cysteine and glutathione conjugates. These relatively polar conjugates were highly active against colon cancer cells, with only slightly lower activity than free parthenolide. In addition to these biosynthetic genes, another gene encoding a costunolide and parthenolide 3?-hydroxylase was identified opening up further options to improve the water solubility of parthenolide and therefore its potential as a drug. PMID:24704560

  6. In Planta mutagenesis determines the functional regions of puroindoline proteins.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    PINA and PINB functionally comprise the wheat (Triticum aestivum L.) Ha locus which controls grain texture and many wheat end-use properties. PINs in their functional forms impart soft texture and a mutation in either PIN is found in all hard wheats. Studies of the PINs mode of action is limited ...

  7. UNIVERSIDAD DE CHILE corr Planta Nombre Rut Grado Lista Pje

    E-print Network

    Rapaport, Iván

    Luis 0051602986 06 1 89,75 09-07-1992 01-01-1971 400000 Página 4 de 96 #12;24-01-2013 UNIVERSIDAD DE Saavedra Díaz Fernando Salvador 0126639961 05 2 80,50 01-06-2003 01-06-2002 080700 4 Profesional N.N. 05 5

  8. In planta modification of the potato tuber cell wall

    Microsoft Academic Search

    R. J. F. J. Oomen

    2003-01-01

    Apart from its well known uses in the human diet a large amount of the grown potatoes (about one third in the Netherlands) is used for the isolation of starch which is used in several food and non-food applications. The cell wall fibres comprise a large portion of the waste material remaining after the starch isolation process. While cell wall

  9. Ecology & Management Effect of Simulated Browsing on Aspen Regeneration: Implications for Restoration

    E-print Network

    Tate, Kenneth

    of California, Davis, CA 95616, USA. Abstract Aspen (Populus tremuloides Michx.) is a disturbance season. Resumen Alamo (Populus tremuloides Michx.) es una planta que depende del disturbio, es resistente, grazing, intensity, Populus tremuloides Mi

  10. The Moon Phases in a Paper Box. (Spanish Title: Las Fases de la Luna en Una Caja de Cartón.) As Fases da Lua Numa Caixa de Papelão

    NASA Astrophysics Data System (ADS)

    de Fátima O. Saraiva, Maria; Amador, Cláudio B.; Kemper, Érico; Goulart, Paulo; Muller, Angela

    2007-12-01

    We present a very simple concrete model to demonstrate the concept of phases of an illuminated body. The main objective of our model is to help the understanding of the Moon phases as viewed from the perspective of an observer on Earth. The material allows the visualization of two important effects: (1st) even though all the time half Moon is illuminated by the Sun, we see different fractions of the illuminated Moon surface, depending on our angle of sight; (2nd) the orientation of the convex part of the Moon in the crescent and waning phases on the sky also depends on our perspective from Earth. The use of a closed box allows one to see the contrast among the different phases with no need of a dark room. We also present a text on the Moon phases, emphasizing the dependence of the aspect of the bright part on the angle of sight. En este trabajo proponemos la construcción de material didáctico de bajo costo para demostración del concepto de fases de un cuerpo iluminado. El principal objetivo de nuestro material es facilitar la comprensión de las fases de la Luna desde la perspectiva de un observador en la Tierra. El material ayuda la visualización de dos efectos importantes: (1º) a pesar de tener siempre la mitad de la Luna (representada por una bolita de espuma plástica o de ping-pong), iluminada por el Sol ( representado por una fuente de luz natural o artificial), vemos diferentes fracciones de su superficie iluminada, dependiendo del ángulo por el cual la vemos; (2º) la orientación del borde convexo de la Luna en las fases Creciente y Menguante también depende de la perspectiva por la cual la miramos desde la Tierra. El uso de una caja cerrada permite observar el contraste entre las diferentes fases sin necesidad de estar en un recinto oscuro. Presentamos también un texto explicativo sobre las fases de la Luna, enfatizando la dependencia de la apariencia de la parte iluminada con el ángulo de visión. Neste trabalho propomos a construção de material didático de baixo custo para demonstração do conceito de fases de um corpo iluminado. O principal objetivo de nosso material é facilitar a compreensão das fases da Lua da perspectiva de um observador na Terra. O material ajuda na visualização de dois efeitos importantes: (1º) mesmo tendo sempre a metade da "Lua" (representada por uma bolinha de isopor ou de ping-pong) iluminada pelo "Sol" (representado por uma fonte de luz natural ou artificial), nós vemos diferentes frações de sua superfície iluminada, dependendo do ângulo pelo qual a olhamos; (2º) a orientação da borda convexa da Lua nas fases Crescente e Minguante também depende da perspectiva pela qual a olhamos da Terra. O uso de uma caixa fechada permite observar o contraste entre as diferentes fases sem necessidade de estar em uma sala escurecida. Apresentamos também um texto explicativo sobre fases da Lua, enfatizando a dependência da aparência da parte iluminada com o ângulo de visada.

  11. Managing Warm-season Improved Pastures (Spanish) 

    E-print Network

    Stichler, Charles; Prostko, Eric P.; Livingston, Stephen

    1999-02-17

    , determina la profundidad y extensi?n de su sistema de ra?ces. En las plantas, el sistema de ra?ces se desarrolla del exceso de energ?a producido por las hojas. En- tre m?s hojas tenga la planta, producir? m?s energ?a y las hojas y ra?ces crecer?n m?s. Hay un... sistema de prioridades en el desarrollo de la planta. Las hojas son m?s importantes que las ra?ces. Cuando una planta tiene un tama?o y n?mero de hojas limitado, se produce poca energ?a para crecimiento. Una planta cuyas hojas hayan sido cortadas...

  12. Una sensacin extraa se haba acomodado en mis sentidos en el momento en el que pis la plataforma. La nave que me haba llevado hasta all no era precisamente

    E-print Network

    Geffner, Hector

    plataforma. La nave que me había llevado hasta allí no era precisamente moderna, lo que provocaba que era un investigador especialista en tecnologías muertas asentado en la Plataforma Nueva Europa que Pasado de la Plataforma Tibidabo, una de las ciudades más grandes del Hemisferio Norte y la situada más

  13. DISEÑO DE UNA ESTRATEGIA DE TRANSFERENCIA DE TECNOLOGÍA EN LA GANADERÍA CAMPESINA DE LA REGIÓN MIXE (AYUUK) EN OAXACA, MÉXICO DESIGN A TECHNOLOGY TRANSFERENCE STRATEGY IN THE FARM LIVESTOCK IN MIXE REGION (AYUUK) OAXACA, MÉXICO

    Microsoft Academic Search

    Juan Antonio-Bautista

    2006-01-01

    RESUMEN Con el propósito de proponer una estrategia de transferencia de tecnología en la ganadería campesina practicada por productores indígenas. Se llevó a cabo la presente investigación en la comunidad indígena de Santo Domingo, Tepuxtepec Mixe, Oaxaca; donde la ganadería campesina se encuentra integrada por aves (gallinas y guajolotes), bovinos, caprinos, ovinos y equinos, siendo notoria la ausencia de los

  14. Memorias del Segundo Simposio Internacional Sobre Polticas, Planificacin y Economa de los Programas de Proteccin Contra Incendios Forestales: Una Visin Global Memorias del Segundo Simposio Internacional Sobre Polticas, Planificacin y Economa de los Progra

    E-print Network

    Standiford, Richard B.

    combustible. El conocimiento de los modelos forestales de gestión de quemas prescritas, permite la aplicación costes de forma comparada entre los sistemas mecánicos y el uso de quemas prescritas, con especial se aborda el estudio de los costes entre ambos sistemas, aportando una matriz de decisión como

  15. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Paris-Sud XI, Université de

    -84-92755-88-2 #12;ÁREA DE CIENCIA POLÍTICA 312 LIDERAZGO POLÍTICO Y GOBERNABILIDAD EN AMÉRICA LATINA: UNA APROXIMACIÓN TEÓRICA Y METODOLÓGICA José Manuel Rivas Otero Resumen En situaciones de crisis de gobernabilidad investigación que busca comparar la gobernabilidad y el estilo de liderazgo de los presidentes/as de Argentina

  16. Memorias del Segundo Simposio Internacional Sobre Polticas, Planificacin y Economa de los Programas de Proteccin Contra Incendios Forestales: Una Visin Global Memorias del Segundo Simposio Internacional Sobre Polticas, Planificacin y Economa de los Progra

    E-print Network

    Standiford, Richard B.

    Forestales: Una Visión Global Análisis Comparativo Entre los Sistemas Antiincendios Forestales en Sicilia. Destacamos que existe gran dificultad para recopilar determinadas informaciones importantes en materia de realizamos puede ayudar a establecer líneas comunes de trabajo, mejorando los sistemas mutuos de lucha contra

  17. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Paris-Sud XI, Université de

    capital humano puesto a disposición de la comunidad y no recogido en los Índices de Desarrollo Humano, así aumentar la producción, sino elevar el capital humano2 . Se entiende que una mejora en la educación, la-84-92755-88-2 #12;ÁREA DE ANTROPOLOGÍA 71 EFECTOS PERVERSOS DEL ÍNDICE DE DESARROLLO HUMANO. EJEMPLO: EL PROGRAMA

  18. El envejecimiento de las poblaciones en Europa o el invierno demogrfico La expresin "vieja Europa" es antigua. Se inscribe en la idea de una antigedad de la civilizacin europea actual en rel

    E-print Network

    Paris-Sud XI, Université de

    , Europa se dist por tres características demográficas: por una parte, es el único continente con menor estudiar la evolución de la estructura por edad en el contin europeo y, sobre todo, los factores que la evolución de la proporción de las personas de 60 años o m de los menores de 20 años para la Unión Europea de

  19. Factores que inciden en las condiciones de vida de la población menor de edad. Una aproximación a partir de la encuesta del Nuevo SISBEN 2004 en el Valle del Cauca

    Microsoft Academic Search

    Luis Fernando Aguado Quintero; Luis Eduardo Girón Cruz; Fernando Salazar Silva

    2005-01-01

    RESUMEN Se presenta un panorama descriptivo sobre el entorno en el que habita la población menor de edad (0-17 años) en el Departamento delValle del Cauca (excepto Cali). Este panorama se construye a partir de indicadores de los que se obtiene una aproximación a la situación en la que se encuentra la vivienda en la que habitan los menores con

  20. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Paris-Sud XI, Université de

    una forma más efectiva un desarrollo turístico sostenible en los países de América Latina. 1 opción de desarrollo económico más viable y sostenible y, en algunos de ellos, la principal fuente de cooperación en el ámbito del turismo y el desarrollo, fruto de la dilatada colaboración entre investigadores

  1. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Boyer, Edmond

    a cabo y los estudios de casos focales realizados, se analizan las acciones de fortalecimiento y sus derechos. Para ello el estudio de casos1 representativos de una trayectoria histórico-política en este", organizado por el Consejo Español de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Políticas y

  2. Red inalmbrica de la Universidad de Alicante La Red Inalmbrica de la UA (eduroam) es una extensin de la red local, disponible en el 99% del Campus de San

    E-print Network

    Escolano, Francisco

    Red inalámbrica de la Universidad de Alicante La Red Inalámbrica de la UA (eduroam) es una extensión de la red local, disponible en el 99% del Campus de San Vicente. eduroam (Educational Roaming) es no puedan utilizarse en la red wireless de la UA o sufran problemas de navegación o conexión. No se ofrecerá

  3. TEMAS PARA UNA BIOETICA LATINOAMERICANA

    PubMed Central

    Yunta, Eduardo Rodriguez

    2009-01-01

    El presente trabajo reflexiona sobre el fundamento filosófico de la bioética como disciplina, método y movimiento desde su contexto histórico y en relación con el contexto social y cultural de la vivencia latinoamericana. PMID:20209030

  4. Hoffmann O., 2004 : "Espacios y regin en el pacfico sur : Hacia la construccin de una sociedad rgional ? pp195-221 in BARBARY O. y URREA F., Gente negra en Colombia: Dinmicas sociopolticas en Cali y el

    E-print Network

    Paris-Sud XI, Université de

    1 Hoffmann O., 2004 : "Espacios y región en el pacífico sur : ¿Hacia la construcción de una en Cali y el Pacífico, CIDSE/IRD/COLCIENCIAS, Medellin, 476p. 1. ESPACIOS, MOVILIDAD Y REGI�N EN EL PACÍFICO SUR 1.1. El espacio regional del Pacífico suroccidental (Fig. 1 ). Figura 1 En este primer

  5. Filling the gap in LNA antisense oligo gapmers: the effects of unlocked nucleic acid (UNA) and 4'-C-hydroxymethyl-DNA modifications on RNase H recruitment and efficacy of an LNA gapmer.

    PubMed

    Fluiter, Kees; Mook, Olaf R F; Vreijling, Jeroen; Langkjaer, Niels; Højland, Torben; Wengel, Jesper; Baas, Frank

    2009-08-01

    Stability against nucleases, affinity for the targeted mRNA and the ability to recruit RNase H are prerequisites for antisense oligonucleotide (AON) applications where gene expression knockdown is required. Typically chimeric gapmer AON designs are used with a central continuous stretch of RNase H recruiting nucleotides (e.g. phosphorothioate DNA), flanked by affinity and stability-enhancing modified nucleotides. However, many types of nucleotide modifications in the central DNA gap can disturb RNase H function. Here we present studies into two different types of nucleotide modifications, a flexible acyclic RNA analog named unlocked nucleic acid (UNA) and 4'-C-hydroxymethyl-DNA in the gap of an LNA (locked nucleic acid) flanked gapmer. We compared the efficacy of mRNA degradation by the gap modified LNA antisense gapmers in cell-free assays and cultured cells. This study shows that both UNA and 4'-C-hydroxymethyl-DNA gap insertions are compatible with RNase H activity when used sparingly. However, multiple 4'-C-hydroxymethyl-DNA modifications are better tolerated by RNase H than multiple UNA modifications in the gap. Furthermore, this report shows that LNA gapmer AONs with multiple 4'-C-hydroxymethyl-DNA moieties in the gap can mediate target knockdown in vivo. PMID:19603119

  6. Learning Astronomy by Playing in a Park. (Spanish Title: Aprender AstronoMía Jugando en Una Plaza.) Aprender Astronomia Brincando em Uma Praça

    NASA Astrophysics Data System (ADS)

    Camino, Néstor

    2012-12-01

    Some public-square games are presented in this paper, considered as didactic modules to help children imagine astronomical processes, based on the concept that learning in Astronomy should be developed to strengthen the relationship of our body with three-dimensional space and time, much in the same way we experience when observing the actual sky, holding a permanent "dialogue" between the actual world and what is to be learned. The games presented (merry-go-rounds and slides) were designed to work on the astronomical concepts related to the translation of the Earth around the Sun, the phases of the Moon and gravity, and on what is perceived by an observer about those phenomena. The description of each game, their physical and astronomical foundations, and a critical comment about their didactical importance are the key parts of the paper. Finally, a recommendation is given about the role teachers should play to be essential partners in the process of learning Astronomy by means of the interaction with these games. Se presentan en este trabajo algunos juegos de plaza, considerados como módulos didácticos para imaginar procesos astronómicos, a partir de la concepción de que el aprendizaje en Astronomía debe desarrollarse fortaleciendo la relación del propio cuerpo con el espacio tridimensional y con el tiempo, tal como se vive al observar el cielo, construyendo un "diálogo" entre el mundo real y los aprendizajes a construir. Los juegos presentados (calesitas y toboganes) fueron diseñados para trabajar sobre la traslación de la Tierra en torno al Sol, las fases de la Luna y la gravedad, y sobre lo que un observador percibe de los mismos. Se da la descripción de cada juego, se discuten sus fundamentos físicos y astronómicos, y se desarrolla una crítica didáctica de los mismos. Finalmente, se comenta el rol que deberían tener los docentes en el acompañamiento a los aprendices en el proceso de interacción con los juegos presentados. Apresentam-se neste trabalho alguns jogos de praça, considerados como módulos didáticos para imaginar processos astronômicos, a partir da concepção de que a aprendizagem em Astronomia deve-se desenvolver fortalecendo a relação do próprio corpo com o espaço tridimensional e com o tempo, tal como se vive ao observar o céu, construindo um "diálogo" entre o mundo real e as aprendizagens a construir. Os jogos apresentados (carrosséis e tobogãs) foram desenhados para trabalhar sobre a translação da Terra em torno do Sol, as fases da Lua e a gravidade, e sobre o que um observador percebe dos mesmos. Dá-se a descrição de cada jogo, discutem-se seus fundamentos físicos e astronômicos, e desenvolve-se uma crítica didática dos mesmos. Finalmente, comenta-se o papel que deveriam ter os docentes no apoio aos aprendizes no processo de interação com os jogos apresentados.

  7. In vivo efficacy and off-target effects of locked nucleic acid (LNA) and unlocked nucleic acid (UNA) modified siRNA and small internally segmented interfering RNA (sisiRNA) in mice bearing human tumor xenografts

    PubMed Central

    Mook, ORF; Vreijling, Jeroen; Wengel, Suzy L; Wengel, Jesper; Zhou, Chuanzheng; Chattopadhyaya, Jyoti; Baas, Frank

    2010-01-01

    The clinical use of small interfering RNA (siRNA) is hampered by poor uptake by tissues and instability in circulation. In addition, off-target effects pose a significant additional problem for therapeutic use of siRNA. Chemical modifications of siRNA have been reported to increase stability and reduce off-target effects enabling possible therapeutic use of siRNA. Recently a large scale direct comparison of the impact of 21 different types of novel chemical modifications on siRNA efficiency and cell viability was published.1 It was found that several types of chemical modifications could enhance siRNA activity beyond that of an unmodified siRNA in vitro. In addition, a novel siRNA design, termed small internally segmented interfering RNA (sisiRNA), composed of an intact antisense strand and segmented guide strand stabilized using LNA was shown to be effective in cell based assays. In the present study we examined the in vivo efficacy of the LNA and UNA modified siRNA and sisiRNA in a mouse model bearing human tumor xenografts. We studied the biodistribution and efficacy of target knockdown in the mouse model. In addition we used whole genome profiling to assess the off-target effects in the liver of the mouse and the tumor xenografts. We report that LNA and UNA modified siRNA and sisiRNA improve the efficacy in target knockdown as compared with unmodified siRNA in the tumor xenografts without formulation. However, the level of off-target gene regulation in both the tumor and the liver correlated with the increase in efficacy in target knockdown, unless the seed region of the siRNA was modified. PMID:21687525

  8. Factors affecting the phytoaccumulation of weathered, soil-borne organic contaminants: analyses at the ex Planta and in Planta sides of the plant root

    Microsoft Academic Search

    MaryJane Incorvia Mattina; William A. Berger; Brian D. Eitzer

    2007-01-01

    Persistent Organic Pollutants (POPs) in the soil–plant system were tracked from their origin in the bulk soil, into the rhizosphere\\u000a soil pore water, to the xylem sap, and up to the aerial plant tissue. Specifically, the profiles of both chiral and achiral\\u000a components of technical chlordane along this continuum were examined in detail for members of the Cucurbitaceae family: Cucurbita

  9. Detección de Virus por Serología y Plantas Indicadoras en el Tubérculo-Semilla y Plantas de Cultivo de Meristemos en Papa (Solanum tuberosum L.) var. Alfa

    Microsoft Academic Search

    Víctor Valenzuela-Herrera; Rafael Bujanos-Muñiz

    2003-01-01

    Viral detection in Alpha potato tubers from Tenango del Valle, State of Mexico, was performed by ELISA and indicator plants. Potato tubers were planted in the greenhouse, and the plants were kept in insect-proof cages; sap was extracted from top leaflets to mechanically inoculate indicator plants for Potato leafroll virus (PLRV), Potato virus A (PVA), Potato virus X (PVX), Potato

  10. Hacia una bibliografía del teatro venezolano colonial.

    E-print Network

    Greymont, Sally J.

    1975-04-01

    teatro en Caracas, Caracas: Tipografía Vargas, 1924. 230 págs. Dauxion-Lavaysse, Jean Francois. A Statistical, Commercial and Political Descrip tion of Venezuela, Trinidad, Margarita, and Tobago, ed. E[duard] B[la- quiere], London: G. and W. B..., Aquiles. "Los programas de teatro," en 400 años de valores teatrales, ed. Aquiles Nazoa. Edición especial, No. 6. Caracas: Círculo Musical, 1967, págs. 43-50. [Disco con texto.] Núñez, Enrique Bernardo. La ciudad de los techos rojos. Caracas: Banco...

  11. Una NAMEA regionale per la Toscana

    Microsoft Academic Search

    Simone Bertini; Renata Caselli; Renato Paniccià

    Summary: Within the broader analysis of the situation in Tuscany, IRPET has begun a study specifically on the interaction between the socio-economic system and the environment. A NAMEA-type matrix (National Accounts Matrix including Environmental Accounts) for the year 2000 was thus set up for Tuscany within the framework of a joint cooperation with ISTAT which could serve as a pilot

  12. Una costruzione del sistema dei numeri reali

    E-print Network

    Enea, Maria Rosaria

    2012-01-01

    There are many ways to construct the field R of real numbers. The most important and famous of these employ Cauchy sequences (Cantor) or cuts (Dedekind) in the field Q of rational numbers. These constructions sometimes overlook important details and often are complicated and cumbersome. In this note, the authors propose an essential, clear and rigorous construction of R from the stucture Q+ of positive rational numbers by the key notion of initial segment.

  13. La economía “negociada” - Una aproximación escandinava

    Microsoft Academic Search

    Klaus Nielsen

    2007-01-01

    Los reflejos mentales se forman según contextos históricos y geográficos. Sin duda, los lugares de la educación y de la vida adulta de un individuo ejercen fuerte influencia sobre su percepción y sus ideas. Si el análisis neoclásico tiene un efecto evidente de homogeneización, también lo tiene para la identidad de un individuo que se formó en ciencias sociales y

  14. Biodiversidad, Desarrollo y Cultura: Una visin integradora

    E-print Network

    Schierup, Mikkel Heide

    Duque Palacio, Universidad del Valle #12;iii Grupo de apoyo: Diana Stefany Escobar, Universidad del, 11-15 Agosto de 2011. ISBN: 978-958-99009-1-8 Compilación: Diana Carolina Acosta Rojas Comité Editorial: Alejandra Jaramillo Sierra Diana Carolina Acosta Rojas Katherine Lorena Rivera Hernández Mariana

  15. Telmaco Talavera Siles Presidente CNU / Rector UNA

    E-print Network

    · Diversification · Add Value · Commercialization · Marketing Nicaraguan Goals #12;Sources: Central Bank%) - Denim pants - Sportswear - Work pants - Knit products - Footwear - Auto wire harnesses (20%) - BPO1 Central American Common Market Nicaragua, Guatema

  16. Ciencia, Sociedad, Soluciones: Una Introduccion al USGS

    USGS Publications Warehouse

    U.S. Geological Survey

    2001-01-01

    El USGS sirve a la nacion de los Estados Unidos proveyendo informacion fidedigna para ? Describir y comprender la Tierra; ? Minimizar la perdida de vidas y propiedades por desastres naturales; ? Manejar los recursos hidrologicos, biologicos, energeticos y minerales; y ? Mejorar y proteger nuestra calidad de vida.

  17. Capital social: una revisión del concepto

    Microsoft Academic Search

    Sander Rangel Jiménez; Jorge Enrique Saiz

    2008-01-01

    Este documento explora el concepto de capital social. Se investiga los aspectos que motivan el desarrollo de la literatura sobre capital social y los esfuerzos por estructurar el concepto de capital social, implementados en la teoría económica. Se argumenta que mientras la literatura de capital social ha producido muchas ideas, un número de problemas conceptuales y teóricos existen en el

  18. UNA PRESENTAZIONE DIDATTICA DELLE GEOMETRIE NON EUCLIDEE

    Microsoft Academic Search

    C. Di Foggia; T. Olivello; R. Prosperi; A. Rotunno

    Sunto: Si affronta il problema dell'insegnamento delle Geometrie non Euclidee nel triennio della scuola media superiore; si propone un per- corso che si sviluppa a partire da un approccio di tipo assiomatico, proseguendo con uno studio comparativo delle geometrie, euclidea, assoluta, iperbolica ed ellittica, e con la presentazione del modello di Poincaré per la dimostrazione della non contraddittorietà della geo-

  19. EL SENTIDO DE UNA EDUCACIÓN EN MEDIOS

    Microsoft Academic Search

    Roxana Morduchowicz

    2003-01-01

    SÍNTESIS: La educación en medios es un campo en torno al cual existen no pocas coincidencias en todo el mundo, basadas en la necesidad de acercar los medios de comunicación a la escuela como objeto de estudio y de análisis en la clase. Sin embargo, esta expresión también suele definir distintas concepcio- nes y enfoques. No todos los países coinciden

  20. Viajes y vacunas: Una precaución necesaria

    Microsoft Academic Search

    Andrés Sanchez Bustelo

    In the last few years it is taking place an important increment of the number of people who travel all over the world, including exotic countries. In this way, about 10 million people, including both immigrants and tourist and diplomatic and businessmen, move annually among Europe and the so-called countries of the third world. Due to these populations movements it

  1. Una “discussione in rete” con Stanley Wilder

    Microsoft Academic Search

    Basile Carla

    2005-01-01

    On 4th January 2005 the USA Information Literacy community is shocked by the Stanley Wilder's article “Information Literacy Makes all the Wrong Assumptions” published on The Chronicle of Higher Education. The article strongly criticises against Information Literacy and academic libraries. As a reaction, an intense debate immediately arises within the USA Information Literacy community through the ILI-L discussion list. The

  2. Superficies bioactivas en implantología: una nueva perspectiva

    Microsoft Academic Search

    A. Cutando; G. Gómez-Moreno; C. Arana

    2007-01-01

    RESUMEN Actualmente, tras años de investigación en implantología, en los cuales se han realizado mu- chos trabajos, en donde se han estudiado diferentes tipos de superficies de implantes, lisas y rugosas, en un intento de mejorar, tanto en el tiempo como en la calidad , la osteointegración, se ha concluido que los implantes de superficie rugosa con un intervalo de

  3. Water conservation and reuse in poultry processing plant—A case study

    Microsoft Academic Search

    E. M. Matsumura; J. C. Mierzwa

    2008-01-01

    Water conservation and water reuse concepts in food industry are presented in this paper. The developed study was in poultry processing plant, which focused water reuse evaluation for only non-potable applications. Although potable water reuse is allowed in some countries and there are international regulations about this practice [Environmental Protection Agency (EPA). Guidelines for Water Reuse; 2004. http:\\/\\/www.epa.gov\\/nrmrl\\/pubs\\/625r04108\\/625r04108.htm], this issue

  4. Soil bacteria augment Arabidopsis photosynthesis by decreasing glucose sensing and abscisic acid levels in planta

    E-print Network

    Paré, Paul W.

    et al., 2003; Smith et al., 1993). The importance of exudates for the metabolic functions of microbes., 2007). Recent discovery of a monosaccha- ride transporter from symbiotic glomeromycotan fungi shed light on how carbohydrates are transported through the symbiotic interface (Schu¨ bler et al., 2006

  5. Perfil dos consumidores de plantas medicinais e condimentares do município de Pato Branco (PR)

    Microsoft Academic Search

    José Abramo Marchese; Fernando Broetto; Lin C. Ming; Rumy Goto; Mirian B. Stefanini; Adinam Galina; Ângela C. Tedesco; Cassiano Conte; Cindia M. Miniuk; Daniel A. Schurt; Emerson Sangaletti; Giovani O. da Silva; Greice Gomes; José A. Bertagnolli; Lucia Francheschi; Maicon L. Cossa; Maires R. D. Moraes; Patrícia M. Lima; Rodrigo Lira; Silvana Costa

    2004-01-01

    The profile of consumers medicinal plants and spices of Pato The profile of supermarket users in Pato Branco city, Paraná State, Brazil, was obtained, related to their preference for medicinal and spice plants, as a subsidy to production by small farmers. The consumers prefer organic products observing the appearance and price of the product, and they go once a week

  6. Identification and analysis of in planta expressed genes of Magnaporthe oryzae

    PubMed Central

    2010-01-01

    Background Infection of plants by pathogens and the subsequent disease development involves substantial changes in the biochemistry and physiology of both partners. Analysis of genes that are expressed during these interactions represents a powerful strategy to obtain insights into the molecular events underlying these changes. We have employed expressed sequence tag (EST) analysis to identify rice genes involved in defense responses against infection by the blast fungus Magnaporthe oryzae and fungal genes involved in infectious growth within the host during a compatible interaction. Results A cDNA library was constructed with RNA from rice leaves (Oryza sativa cv. Hwacheong) infected with M. oryzae strain KJ201. To enrich for fungal genes, subtraction library using PCR-based suppression subtractive hybridization was constructed with RNA from infected rice leaves as a tester and that from uninfected rice leaves as the driver. A total of 4,148 clones from two libraries were sequenced to generate 2,302 non-redundant ESTs. Of these, 712 and 1,562 ESTs could be identified to encode fungal and rice genes, respectively. To predict gene function, Gene Ontology (GO) analysis was applied, with 31% and 32% of rice and fungal ESTs being assigned to GO terms, respectively. One hundred uniESTs were found to be specific to fungal infection EST. More than 80 full-length fungal cDNA sequences were used to validate ab initio annotated gene model of M. oryzae genome sequence. Conclusion This study shows the power of ESTs to refine genome annotation and functional characterization. Results of this work have advanced our understanding of the molecular mechanisms underpinning fungal-plant interactions and formed the basis for new hypothesis. PMID:20146797

  7. Espécies ativas de oxigênio na resposta de defesa de plantas a patógenos

    Microsoft Academic Search

    Mário Lúcio V. Resende; Sônia M. L. Salgado; Zuleide M. Chaves

    2003-01-01

    Reactive oxygen species on plant defense responses to pathogens Oxidative burst is a very quick plant defense response after pathogen recognition, leading to hypersensitiv e reaction (HR). This response is due to generation of reactive oxygen species (ROS), such as H 2O2 , O 2-, and OH-. Several roles in plant defense have been proposed for ROS. Hidrogen peroxide (H2O2),

  8. Regioselectivity of glucosylation of caffeic acid by a UDP-glucose:glucosyltransferase is maintained in planta.

    PubMed Central

    Lim, Eng-Kiat; Higgins, Gillian S; Li, Yi; Bowles, Dianna J

    2003-01-01

    Caffeic acid is a phenylpropanoid playing an important role in the pathways leading to lignin synthesis and the production of a wide variety of secondary metabolites. The compound is also an antioxidant and has potential utility as a general protectant against free radicals. Three glucosylated forms of caffeic acid are known to exist: the 3- O - and 4- O -glucosides and the glucose ester. This study describes for the first time a glucosyltransferase [UDP-glucose:glucosyltransferase (UGT)] that is specific for the 3-hydroxyl, and not the 4-hydroxyl, position of caffeic acid. The UGT sequence of Arabidopsis, UGT71C1, has been expressed as a recombinant fusion protein in Escherichia coli, purified and assayed against a range of substrates in vitro. The assay confirmed that caffeic acid as the preferred substrate when compared with other hydroxycinnamates, although UGT71C1 also exhibited substantial activity towards flavonoid substrates, known to have structural features that can be recognized by many different UGTs. The expression of UGT71C1 in transgenic Arabidopsis was driven by the constitutive cauliflower mosaic virus 35 S (CaMV35S) promoter. Nine independent transgenic lines were taken to homozygosity and characterized by Northern-blot analysis, assay of enzyme activity in leaf extracts and HPLC analysis of the glucosides. The level of expression of UGT71C1 was enhanced considerably in several lines, leading to a higher level of the corresponding enzyme activity and a higher level of caffeoyl-3- O -glucoside. The data are discussed in the context of the utility of UGTs for natural product biotransformations. PMID:12741958

  9. Acción biocida de plantas de Piper tuberculatum Jacq. sobre Diatraea saccharalis (Lepidóptera, Pyralidae)

    Microsoft Academic Search

    Gladys V. Soberón; Consuelo Rojas; Jorge Saavedra; Massuo J. Kato; Guillermo E. Delgado

    The biocid action of DCM:MeOH (2:1), EtOH and aqueous extracts of leaves, stems and mature spikes (with fruits and seeds) of field plants and DCM:MeOH (2:1) extract of in vitro plants of Piper tuberculatum on III larval stage of Diatraea saccharalis was evaluated. The method was by inoculating the previously eluted extract with distillate water as topic applications on the

  10. Carotenoid In Planta Development, Storage, and Bioaccessibility: A Comprehensive Approach to Nutrient Analysis

    E-print Network

    Jeffery, Jennifer L.

    2010-01-14

    Phytochemical and Nutrient Accessibility ..................................... 6 II CAROTENOID BIOSYNTHESIS IN SEVEN VARIETIES OF DEVELOPING WATERMELON (CITRULLUS LANATUS (THUNB.) MATSUM. & NANAI): A DISCUSSION OF FLESH COLOR AND PLOIDY EFFECTS... ...................................................................................... 31 ix ix CHAPTER Page III GENOTYPIC EFFECTS ON WATERMELON (CITRULLUS LANATUS (THUNB.) MATSUM. & NANAI) CAROTENOID...

  11. Planta (2007) 227:167175 DOI 10.1007/s00425-007-0604-1

    E-print Network

    Quatrano, Ralph S.

    2007-01-01

    to wild type embryos. Expression of AtEm1 and AtEm6 was strongly induced by abscisic acid in callus of the transcriptional complex in vitro. Keywords 14-3-3 Proteins · ABI3 · Abscisic acid · Em · Embryo calli Abbreviations ABA Abscisic acid ChIP Chromatin immunoprecipitation DAP Days after pollination EMSA

  12. Planta (2007) 225:863872 DOI 10.1007/s00425-006-0398-6

    E-print Network

    Quatrano, Ralph S.

    2007-01-01

    and transactivation. Keywords Abscisic acid · Barley · Em · Nuclear localization · Physcomitrella · VP1 Abbreviations ABA Abscisic acid ABI3 Abscisic acid insensitive 3 ABI5 Abscisic acid insensitive 5 GFP Green signal VP1 Viviparous 1 Introduction The phytohormone abscisic acid (ABA) plays a vital role in plant

  13. Hydraulic properties of Zinnia elegans : from cellular development in vitro to performance in planta

    Microsoft Academic Search

    P. Twumasi

    2007-01-01

    The water status in plants is dependent on the xylem hydraulic conductance. In cut flowers, for example, the preservation of continuous hydraulic conductance is important for maintaining longer vase life, an important index for cut flower quality. Many factors, such as stomata performance, root water uptake efficiency, temperature, light quality and humidity, influence the efficiency of water transport in the

  14. Variação sazonal em antioxidantes em folhas de plantas jovens de Caesalpinia echinata Lam. (pau-brasil)

    Microsoft Academic Search

    Patricia Bulbovas; Mirian C. S. Rinaldi; Welington B. C. Delitti; Marisa Domingos

    2005-01-01

    Seasonal variation in antioxidants in leaves of young plants of Caesalpinia echinata Lam. (brazilwood)). The urban environmental factors generate conditions of oxidative stress in plants. Cellular antioxidants, such as ascorbic acid (AA), thiols (Ti), peroxidases (POD) and superoxide dismutase (SOD) are induced to different levels depending on their capacity to tolerate stressing conditions. In the first part of this study

  15. Chlamydiae Has Contributed at Least 55 Genes to Plantae with Predominantly Plastid Functions

    Microsoft Academic Search

    Ahmed Moustafa; Adrian Reyes-Prieto; Debashish Bhattacharya; Robert Desalle

    2008-01-01

    Not Available Bibtex entry for this abstract Preferred format for this abstract (see Preferences) Find Similar Abstracts: Use: Authors Title Return: Query Results Return items starting with number Query Form Database: Astronomy Physics arXiv e-prints

  16. "In planta" regulation of the "Arabidopsis" Ca(2+)/H(+) antiporter CAX1

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vacuolar localized Ca(2+)/H(+) exchangers such as "Arabidopsis thaliana" cation exchanger 1 (CAX1) play important roles in Ca(2+) homeostasis. When expressed in yeast, CAX1 is regulated via an N-terminal autoinhibitory domain. In yeast expression assays, a 36 amino acid N-terminal truncation of CAX1...

  17. Parâmetros de planta para aprimorar o manejo da adubação nitrogenada de cobertura em milho

    Microsoft Academic Search

    Lisandro Rambo; Paulo Regis Ferreira da Silva; Gilber Argenta; Luís Sangoi

    2004-01-01

    Maize is one of the most demanding grasses in terms of nitrogen fertilization. There is a need to estimate accurately the amount of N applied to the soil so that this crop can be properly supplied during its whole cycle. The soil organic matter content, grain yield expectation and N contribution from the decay of the preceding crop are the

  18. In Planta Protein Sialylation through Overexpression of the Respective Mammalian Pathway*

    PubMed Central

    Castilho, Alexandra; Strasser, Richard; Stadlmann, Johannes; Grass, Josephine; Jez, Jakub; Gattinger, Pia; Kunert, Renate; Quendler, Heribert; Pabst, Martin; Leonard, Renaud; Altmann, Friedrich; Steinkellner, Herta

    2010-01-01

    Many therapeutic proteins are glycosylated and require terminal sialylation to attain full biological activity. Current manufacturing methods based on mammalian cell culture allow only limited control of this important posttranslational modification, which may lead to the generation of products with low efficacy. Here we report in vivo protein sialylation in plants, which have been shown to be well suited for the efficient generation of complex mammalian glycoproteins. This was achieved by the introduction of an entire mammalian biosynthetic pathway in Nicotiana benthamiana, comprising the coordinated expression of the genes for (i) biosynthesis, (ii) activation, (iii) transport, and (iv) transfer of Neu5Ac to terminal galactose. We show the transient overexpression and functional integrity of six mammalian proteins that act at various stages of the biosynthetic pathway and demonstrate their correct subcellular localization. Co-expression of these genes with a therapeutic glycoprotein, a human monoclonal antibody, resulted in quantitative sialylation of the Fc domain. Sialylation was at great uniformity when glycosylation mutants that lack plant-specific N-glycan residues were used as expression hosts. Finally, we demonstrate efficient neutralization activity of the sialylated monoclonal antibody, indicating full functional integrity of the reporter protein. We report for the first time the incorporation of the entire biosynthetic pathway for protein sialylation in a multicellular organism naturally lacking sialylated glycoconjugates. Besides the biotechnological impact of the achievement, this work may serve as a general model for the manipulation of complex traits into plants. PMID:20305285

  19. In planta transient expression as a system for genetic and biochemical analyses of chlorophyll biosynthesis

    Microsoft Academic Search

    Ruairidh JH Sawers; Phyllis R Farmer; Peter Moffett; Thomas P Brutnell

    2006-01-01

    BACKGROUND: Mg chelatase is a multi-subunit enzyme that catalyses the first committed step of chlorophyll biosynthesis. Studies in higher plants and algae indicate that the Mg chelatase reaction product, Mg-protoporphyrin IX plays an essential role in nuclear-plastid interactions. A number of Mg chelatase mutants have been isolated from higher plants, including semi-dominant alleles of ChlI, the gene encoding the I

  20. Planta (2009) 230:111 DOI 10.1007/s00425-009-0921-7

    E-print Network

    Tholl, Dorothea

    2009-01-01

    -induced volatile formation in Arabidopsis lyrata ssp. petraea, a perennial, outcrossing relative of A. thaliana AlCarS Arabidopsis lyrata ssp. petraea (E)- -caryophyllene synthase AtTPS21 A. thaliana terpene. The self-pollinating annual, Arabidopsis thaliana, and its relative, the outcrossing perennial, Arabidopsis

  1. Planta (2008) 228:411425 DOI 10.1007/s00425-008-0746-9

    E-print Network

    Sheen, Jen

    2008-01-01

    and evolutionary features of the hexokinase gene family in Arabidopsis Abhijit Karve · Bradley L. Rauh · Xiaoxia Arabidopsis hexokinase1 (HXK1) is a moon- lighting protein that has separable functions in glucose signaling of numerous existing changes. Sliding windows analyses of coding sequences in A. thaliana and A. lyrata ssp

  2. Pharma-Planta: road testing the developing regulatory guidelines for plant-made pharmaceuticals

    Microsoft Academic Search

    Penelope Amelia Claire Sparrow; Judith A. Irwin; Phil J. Dale; Richard M. Twyman; Julian K. C. Ma

    2007-01-01

    Significant advances over the last few years have seen plant-made pharmaceuticals (PMPs) move from the exploratory research\\u000a phase towards clinical trials, with the first commercial products for human use expected to reach the market by 2009. Europe\\u000a has yet to witness the commercial application of PMP technology, although at least one product has begun phase II clinical\\u000a trials with others

  3. Planta (2007) 226:12991310 DOI 10.1007/s00425-007-0551-x

    E-print Network

    Plaxton, William

    2007-01-01

    Phosphoenolpyruvate PEPC PEP carboxylase PKc Cytosolic pyruvate kinase PPCK PEP carboxylase kinase PP2Ac Catalytic carboxylase protein kinase from developing castor oil seeds: partial puriWcation, characterization carboxylase (PEPC, EC 4.1.1.31) protein kinase (PPCK) was puriWed »1,500-fold from developing castor oil seeds

  4. Phylogenetic Relationships of Fungi, Plantae, and Animalia Inferred from Homologous Comparison of Ribosomal Proteins

    Microsoft Academic Search

    Gabriel Bittar

    1998-01-01

    .   The complete set of available ribosomal proteins was utilized, at both the peptidic and the nucleotidic level, to establish\\u000a that plants and metazoans form two sister clades relative to fungi. Different phylogenetic inference methods are applied to\\u000a the sequence data, using archeans as the outgroup. The evolutionary length of the internal branch within the eukaryotic crown\\u000a trichotomy is demonstrated

  5. Sexual mating preferences in vitro and in planta among Japanese isolates of Phytophthora infestans

    Microsoft Academic Search

    Kiyotaka Gotoh; Seishi Akino; Takako Kiyoshi; Shigeo Naito

    2005-01-01

    The sexual preferences of Japanese isolates of Phytophthora infestans were determined by mating on agar, in broth, or in plants. The influence of their sexual preference was confirmed in the host tissues. Three wild-type isolates and a ß-glucuronidase (GUS) transformant were co-cultured to identify the origin of antheridia and oogonia. Japanese A1 isolate had a unique sexual preference compared with

  6. In planta quantification of endoreduplication using fluorescent in situ hybridization (FISH).

    PubMed

    Bourdon, Matthieu; Coriton, Olivier; Pirrello, Julien; Cheniclet, Catherine; Brown, Spencer C; Poujol, Christel; Chevalier, Christian; Renaudin, Jean-Pierre; Frangne, Nathalie

    2011-06-01

    Endopolyploidy, i.e. amplification of the genome in the absence of mitosis, occurs in many plant species and happens along with organ and cell differentiation. Deciphering the functional roles of endopolyploidy is hampered by the fact that polyploid tissues generally comprise cells with various ploidy levels. In some fleshy fruits (amongst them tomato fruit) the ploidy levels present at the end of development range from 2C to 256C in the same tissue. To investigate the temporal and spatial distribution of endopolyploidy it is necessary to address the DNA content of individual nuclei in situ. Conventional methods such as fluorometry or densitometry can be used for some tissues displaying favorable characteristics, e.g. small cells, small nuclei, organization in a monolayer, but high levels of varying polyploidy are usually associated with large sizes of nuclei and cells, in a complex three dimensional (3-D) organization of the tissues. The conventional methods are inadequate for such tissue, becoming semi-quantitative and imprecise. We report here the development of a new method based on fluorescent in situ bacterial artificial chromosome hybridizations that allows the in situ determination of the DNA ploidy level of individual nuclei. This method relies on the counting of hybridization signals and not on intensity measurements and is expected to provide an alternative method for mapping endopolyploidy patterns in mature, 3-D organized plant tissues as illustrated by the analysis of ploidy level and cell size in pericarp from mature green tomato fruit. PMID:21418357

  7. Crescimento e análise do potencial antifúngico em plantas de Cissus verticillata (L.) Nicolson & Jarvis (Vitaceae)

    Microsoft Academic Search

    L. SILVA

    Growth and analysis of antifungical potential of Cissus verticillata (L.) Nicolson & Jarvis (Vitaceae). Photoperiod is an environmental factor that signalizes changes of seasons and induces physiological responses in plants. This can lead to the adaptation to the environment, as well as to the synthesis of secondary compounds. Cissus verticillata is known as \\

  8. Carotenoid In Planta Development, Storage, and Bioaccessibility: A Comprehensive Approach to Nutrient Analysis 

    E-print Network

    Jeffery, Jennifer L.

    2010-01-14

    , tomato, and melon have the largest cells. Sweet potato, butternut squash, carrot, and mango have the most fibrous cell walls; mango and papaya additionally had the thickest walls. Chromoplast globular, tubular, crystalline, and membranous substructures...

  9. In Planta visual monitoring of green fluorescent protein in transgenic rice plants.

    PubMed

    Chung, B C; Kim, J K; Nahm, B H; Lee, C H

    2000-08-31

    The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is a widely used reporter that can be directly visualized in the living cells in both animals and plants. We inserted a synthetic gene (sgfp) encoding a modified form of the GFP into expression vector, Act1-sgfp for the direct expression of GFP which is easily detectable in rice plants. Green fluorescence emitted from GFP could be visualized in calli, dry seeds, roots and seedlings with green shoots of transgenic rice plants. In our visualization system with a charge-coupled device camera, band-pass filters and a light source, the presence of red chlorophyll autofluorescence from chloroplasts did not alter the green fluorescence of GFP. These results demonstrate that GFP could be used as a non-destructive visual selection marker for examining gene expression in transformed calli, dry seeds and young plants. PMID:10987138

  10. In planta changes in protein phosphorylation induced by the plant hormone abscisic acid

    PubMed Central

    Kline, Kelli G.; Barrett-Wilt, Gregory A.; Sussman, Michael R.

    2010-01-01

    Abscisic acid (ABA) is a hormone that controls seed dormancy and germination as well as the overall plant response to important environmental stresses such as drought. Recent studies have demonstrated that the ABA-bound receptor binds to and inhibits a class of protein phosphatases. To identify more broadly the phosphoproteins affected by this hormone in vivo, we used 14N/15N metabolic labeling to perform a quantitative untargeted mass spectrometric analysis of the Arabidopsis thaliana phosphoproteome following ABA treatment. We found that 50 different phosphopeptides had their phosphorylation state significantly altered by ABA over a treatment period lasting 5–30 min. Among these changes were increases in phosphorylation of subfamily 2 SNF1-related kinases and ABA-responsive basic leucine zipper transcription factors implicated in ABA signaling by previous in vitro studies. Furthermore, four members of the aquaporin family showed decreased phosphorylation at a carboxy-terminal serine which is predicted to cause closure of the water-transporting aquaporin gate, consistent with ABA's role in ameliorating the effect of drought. Finally, more than 20 proteins not previously known to be involved with ABA were found to have significantly altered phosphorylation levels. Many of these changes are phosphorylation decreases, indicating that an expanded model of ABA signaling, beyond simple phosphatase inhibition, may be necessary. This quantitative proteomics dataset provides a more comprehensive, albeit incomplete, view both of the protein targets whose biochemical activities are likely to be controlled by ABA and of the nature of the emerging phosphorylation and dephosphorylation cascades triggered by this hormone. PMID:20733066

  11. Dynamic Root Exudation of Sorgoleone and Its in Planta Mechanism of Action

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The oily droplets exuding from the root hairs of sorghum are composed of a 1:1 ratio of sorgoleone and its lipid resorcinol analog. The production and release appear to be suppressed when 15 to 20 µg/mg dry weight accumulates at the tip of the root hairs. However, more exudate is produced followin...

  12. CRECIMIENTO DE PLANTAS INJERTADAS JÓVENES DE DOS CULTIVARES DE NÍSPERO DESPUÉS DEL PRENDIMIENTO

    Microsoft Academic Search

    Norkys Mez; Dámaso Bautista

    Growth of young grafted plants of two cultivars of sapodilla after the healing The grafting, growth and branching of the main axis of sapodilla (Manilkara zapota van Royen) was studied in the cvs. Santiago and Delfina. The trial was carried out in the experimental field of the Graduate School of Horticulture, in Tarabana, Lara State (10º 01' N, 500 masl),

  13. 14 MaIG 19 JunY 2014 Edifici Biblioteca General -planta baIXa

    E-print Network

    Escolano, Francisco

    /SAB El sabor del sake = Sanma no Aji MD 791.223.2/OZU/SAB El sabor del té verde con arroz = Ochazuke no aji Hoshino, Yoshihiro MD 791.223.2/HOS/TIE La tienda maldita : (abierto las 24 h.) Ichikawa, Kon MD

  14. Regulation and mechanism of potassium release from barley roots: an in planta 42

    E-print Network

    Britto, Dev T.

    Phytologist (2010) 188: 1028­1038 doi: 10.1111/j.1469-8137.2010.03436.x Key words: barley (Hordeum vulgare from roots of intact barley (Hordeum vulgare), in the context of recent discoveries in the molecular

  15. Modes of action associated with microbially induced in planta suppression of plant-parasitic nematodes

    Microsoft Academic Search

    R. A. Sikora; K. Schäfer; A. A. Dababat

    2007-01-01

    This article discusses the extensive research that has been conducted on the use of mutualistic bacterial and fungal endophytes\\u000a for the biological control of plant-parasitic nematodes. This review deals in particular with the modes of action of multitrophic\\u000a interactions involving endophytic bacteria or fungi that have biological control activity towards the root-knot nematode,\\u000a Meloidogyne incognita, and the potato cyst nematode,

  16. UNIVERSIDAD SIMN BOLVAR VICERRECTORADO ADMINISTRATIVO

    E-print Network

    Vásquez, Carlos

    UNIVERSIDAD SIMÓN BOLÍVAR VICERRECTORADO ADMINISTRATIVO DIRECCIÓN DE PLANTA FÍSICA PROPUESTA DE UNA) adscrita al Vicerrectorado Administrativo, desde su creación en el año 2001 su estructura no correspondía a la naturaleza de sus funciones, ni a los procesos medulares que la caracterizaban, lo que ocasionaba

  17. KNAPP ET AL. Rev. peru. biol. Nmero especial 13(2): 612s -643s (Diciembre 2006)

    E-print Network

    Spooner, David

    endémicas del Perú. Ed.: Blanca León et al. © Facultad de Ciencias Biológicas UNMSM Solanaceae endémicas del (holotipo+1). Resumen La familia Solanaceae es una de las más ricas en especies en la flora peruana, siendo: Solanaceae, Solanum, Nolana, Jaltomata, Perú, endemismo, plantas endémicas. Abstract The Solanaceae are among

  18. NEED HELP WITH YOUR APPLICATION? Visit HealthCare.gov or call us at 1-800-XXX-XXXX. Para obtener una copia de este formulario en Espaol, llame 1-800-XXX-XXXX. If you need help in a language other than English, call 1-800-XXX-XXXX and tell the

    E-print Network

    Acton, Scott

    NEED HELP WITH YOUR APPLICATION? Visit HealthCare.gov or call us at 1-800-XXX-XXXX. Para obtener una copia de este formulario en Español, llame 1-800-XXX-XXXX. If you need help in a language other than English, call 1-800-XXX-XXXX and tell the customer service representative the language you need

  19. Master d'Ocanographie UE 310 `Invasions et transferts biologiques'

    E-print Network

    Boudouresque, Charles F.

    Streptobionta Euglyphids Retaria Cercobionta ARCHAEPLASTIDAARCHAEPLASTIDA (= PLANTAE)(= PLANTAE) Ellobiopsidae (Boudouresque, original) The 10 Eukaryote kingdoms Lobosa Mycetobionta Chytridiomycota Fungi Choanoflagellata (= PLANTAE)(= PLANTAE) Ellobiopsidae Bicosoecida Picobiliphyta ? Katablepharida CRYPTO- Trentepohlio- phyceae

  20. Las dietas hipocalóricas se asocian a una ingesta baja de algunas vitaminas: una revisión

    Microsoft Academic Search

    Eduard Baladia; Maria Manera; Julio Basulto

    2008-01-01

    In this article, the vitamin supply provided by different hypocaloric diets, whether they are controlled or not by a professional, is analysed through a bibliographic review. The bibliographic sources that affirm that vitamin and mineral supplements must be taken when hypocaloric diets are followed provide no scientific study that supports this statement. Neither has evidence been found that sustains this

  1. Una novedosa herramienta estadística para desentrañar las características intrínsecas de una distribución de datos astrofísicos

    NASA Astrophysics Data System (ADS)

    Ferrero, I.; Piatti, A. E.

    The statistical analysis of a sample of data includes the collection; the analysis and the interpretation of them. For example; the construction of histograms has proven to be a powerful tool in statistics; from which it is possible to obtain an overview of the distribution of the data sample considered. In some circumstances; however; the results often depend on the way they are constructed (e.g. size of the bins used; etc.) leading to a variety of possible interpretations. Specifically; this paper presents a statistical method that allows to unravel the intrinsic distribution of a n-dimensional data distribution from "adaptive bins" and the consideration of the errors of the data used. FULL TEXT IN SPANISH

  2. QU ES UNA BEBIDA ESTNDAR? En los Estados Unidos, una bebida estndar es cualquier

    E-print Network

    Bandettini, Peter A.

    ? TRASTORNOS POR EL USO DE ALCOHOL EN LOS BEBEDORES QUE TIENEN ESTE PATRÓN? Basado en los siguientes límites solamente los limites semanales. La prevalencia combinada de uso y trastornos de alcohol en este grupo es de

  3. Detectores radiográficos digitales inalámbricos en una sala de radiología de urgencias. Una solución eficaz

    Microsoft Academic Search

    M. Garrido Blázquez; M. Agulla Otero; F. J. Rodríguez Recio; R. Torres Cabrera; I. Hernando González

    ObjectivesTo evaluate the implementation of a flat panel digital radiolography (DR) system with WiFi technology in an emergency radiology area in which a computed radiography (CR) system was previously used. We analyzed aspects related to image quality, radiation dose, workflow, and ergonomics.

  4. The Understanding of Astronomy Concepts by Students from Basic Education of a Public School. (Spanish Title: El Entendimiento de Conceptos de Aastronmía Por Los Alumnos de Educación Básica en Una Escuela Pública.) O Entendimento de Conceitos de Astronomia Por Alunos da Educa&wcedil;ão Básica: O Caso de Uma Escola Pública Brasileira

    NASA Astrophysics Data System (ADS)

    Iria Machado, Daniel; dos Santos, Carlos

    2011-07-01

    We present the results obtained in a research on the comprehension of basic astronomical concepts, in which 561 students from fifth grade middle school to third grade high school of a public school of the city of Foz do Iguaçu (Brazil) took part. A test with 20 multiple-choice questions was applied to indentify the most common conceptions expressed by the students. This test was elaborated based on the literature about misconceptions and covered the following topics: the day-night cycle; the time zones; the seasons of the year; the phases of the Moon; the movement of the Moon; the apparent movement of the Sun in the celestial sphere; the eclipses; the dimensions and distances in the Universe; the brightness of the stars and its observation from Earth. Though a small progress was verified in the proportion of scientifically acceptable answers when comparing the eighth grade of middle school to the fifth, and the third grade of high school to the first, there was an overall predominance of alternative conceptions regarding most of the explored subjects, which persisted up to the last year of secondary school. The comparison to data found in this research made in other socio-cultural contexts revealed, in many aspects, similar notions and difficulties revealed by the students. Se presentan los resultados de una investigación sobre la comprensión de conceptos astronómicos básicos, en la cual participaron 561 estudiantes que cursaban entre el quinto grado de la enseñanza primaria y el tercer año de la enseñanza secundaria de una escuela pública de la ciudad de Foz do Iguaçu (Brasil). Se utilizó un test de 20 preguntas de opción múltiple para identificar las concepciones más comunes expresadas por los estudiantes. Este instrumento de recolección de datos se desarrolló en base a la literatura sobre las concepciones alternativas y trató los siguientes temas: el ciclo día-noche, los husos horarios, las estaciones del año, las fases de la Luna, el movimiento de la Luna, el movimiento aparente del Sol sobre la esfera celeste, los eclipses, las dimensiones y las distancias en el Universo, el brillo de las estrellas y su observación de la Tierra. Si bien ha habido un pequeño progreso en la proporción de respuestas aceptables científicamente cuando se cotejó el octavo grado de la enseñanza primaria con el quinto, y el último año de la enseñanza secundaria con el primero, se observó un predominio de las concepciones alternativas en relación a la mayoría de los temas explorados, que continuó hasta los últimos años de la educación básica. Una comparación con los datos encontrados en investigaciones realizadas en otros contextos socioculturales revelaron, en muchos aspectos, conceptos y dificultades similares expresadas por los estudiantes. Apresentam-se os resultados de uma investigação sobre a compreensão de conceitos astronômicos básicos, da qual participaram 561 estudantes da quinta série do Ensino Fundamental ao terceiro ano do Ensino Médio de uma escola pública da cidade de Foz do Iguaçu. Um teste com 20 questões de múltipla escolha foi aplicado para identificar as concepções mais comuns expressadas pelos alunos. Esse instrumento de coleta de dados foi elaborado com base na literatura sobre concepções alternativas e abordou os seguintes temas: o ciclo dia-noite; os fusos horários; as estações do ano; as fases da Lua; o movimento da Lua; o movimento aparente do Sol na esfera celeste; os eclipses; as dimensões e distâncias no Universo; o brilho das estrelas e sua observação da Terra. Embora se tenha verificado um pequeno progresso na proporção de respostas cientificamente aceitáveis ao se cotejar a oitava série do Ensino Fundamental com a quinta, e a terceira série do Ensino Médio com a primeira, houve o predomínio de concepções alternativas em relação à maior parte dos temas explorados, as quais persistiram até o último ano da Educação Básica. A comparação com dados encontrados em investigações realizadas em outros contextos socioculturais revelaram, em m

  5. Plantas con Flores: Claves para la Evolución de la Tierra y Para el Bienestar Humano

    NSDL National Science Digital Library

    Pamela Soltis (University of Florida, Gainesville; )

    2006-03-01

    The issue-focused interview discusses how angiosperms, or flowering plants, are important because they hold clues to the evolutionary history of our planet, provide sources of food, medicine, and shelter to humans, and serve as food and habitat for many other organisms.

  6. In planta imaging of ?9-tetrahydrocannabinolic acid in Cannabis sativa L. with hyperspectral coherent anti-Stokes Raman scattering microscopy

    NASA Astrophysics Data System (ADS)

    Garbacik, Erik T.; Korai, Roza P.; Frater, Eric H.; Korterik, Jeroen P.; Otto, Cees; Offerhaus, Herman L.

    2013-04-01

    Nature has developed many pathways to produce medicinal products of extraordinary potency and specificity with significantly higher efficiencies than current synthetic methods can achieve. Identification of these mechanisms and their precise locations within plants could substantially increase the yield of a number of natural pharmaceutics. We report label-free imaging of ?9-tetrahydrocannabinolic acid (THCa) in Cannabis sativa L. using coherent anti-Stokes Raman scattering microscopy. In line with previous observations we find high concentrations of THCa in pistillate flowering bodies and relatively low amounts within flowering bracts. Surprisingly, we find differences in the local morphologies of the THCa-containing bodies: organelles within bracts are large, diffuse, and spheroidal, whereas in pistillate flowers they are generally compact, dense, and have heterogeneous structures. We have also identified two distinct vibrational signatures associated with THCa, both in pure crystalline form and within Cannabis plants; at present the exact natures of these spectra remain an open question.

  7. 27ECOLOGIA COMPORTAMENTAL NA INTERFACE FORMIGA-PLANTA-HERBVORO Oecol. Bras., 13(1): 27-44, 2009

    E-print Network

    Oliveira, Paulo S.

    a biologia e o comportamento de larvas de Lepidoptera. Palavras-chave: Espaço livre de inimigos, Formicidae by ants. Because they are the principle predators among foliage, ants can strongly affect the communities on plants through antagonistic interactions (e.g. aggressiveness, predation), (2) ants can create an enemy

  8. Cdigos UNESCO: 241712 241714 2407 Frecuencia del curso: Anual BIOLOGA DE LA REPRODUCCIN EN PLANTAS SUPERIORES. IMPLICACIONES AGRONMICAS Y

    E-print Network

    meristemo: genes homeóticos. Mutantes de la inducción floral. Determinación del sexo. Flores hermafroditas, monoicas y dioicas. Mecanismos para evitar la endogamia. Mecanismos genéticos de determinación del sexo. II

  9. Rapid in planta detection of Chalara fraxinea by a real-time PCR assay using a dual-labelled probe

    Microsoft Academic Search

    Renaud Ioos; Tadeusz Kowalski; Claude Husson; Ottmar Holdenrieder

    2009-01-01

    Chalara fraxinea is a fungus currently threatening ash trees (Fraxinus excelsior) in several European countries. This emerging pathogen was assigned to the EPPO’s alert list and therefore accurate detection\\u000a and identification tools are needed. Because of its slow growth rate on agar media and the frequent presence of fast-growing\\u000a saprotrophic fungi within the host tissue, classical isolation techniques are time-consuming

  10. An In Planta-Expressed Polyketide Synthase Produces (R)-Mellein in the Wheat Pathogen Parastagonospora nodorum

    PubMed Central

    Krill, Christian; Barrow, Russell A.; Chen, Shasha; Trengove, Robert; Oliver, Richard P.; Solomon, Peter S.

    2014-01-01

    Parastagonospora nodorum is a pathogen of wheat that affects yields globally. Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 (SN477), in P. nodorum that is highly upregulated during infection of wheat leaves. Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as (R)-mellein and (R)-O-methylmellein. Using a Saccharomyces cerevisiae yeast heterologous expression system, we successfully demonstrated that SN477 is the only enzyme required for the production of (R)-mellein. This is the first identification of a fungal PKS that is responsible for the synthesis of (R)-mellein. The P. nodorum ?SN477 mutant did not show any significant difference from the wild-type strain in its virulence against wheat. However, (R)-mellein at 200 ?g/ml inhibited the germination of wheat (Triticum aestivum) and barrel medic (Medicago truncatula) seeds. Comparative sequence analysis identified the presence of mellein synthase (MLNS) homologues in several Dothideomycetes and two sodariomycete genera. Phylogenetic analysis suggests that the MLNSs in fungi and bacteria evolved convergently from fungal and bacterial 6-methylsalicylic acid synthases. PMID:25326302

  11. Development of a Mild Viral Expression System for Gain-Of-Function Study of Phytoplasma Effector In Planta.

    PubMed

    Hu, Sin-Fen; Huang, Yu-Hsin; Lin, Chan-Pin; Liu, Li-Yu Daisy; Hong, Syuan-Fei; Yang, Chiao-Yin; Lo, Hsiao-Feng; Tseng, Ting-Yu; Chen, Wei-Yao; Lin, Shih-Shun

    2015-01-01

    PHYL1 and SAP54 are orthologs of pathogenic effectors of Aster yellow witches'-broom (AYWB) phytoplasma and Peanut witches'-broom (PnWB) phytoplasma, respectively. These effectors cause virescence and phyllody symptoms (hereafter leafy flower) in phytoplasma-infected plants. T0 lines of transgenic Arabidopsis expressing the PHYL1 or SAP54 genes (PHYL1 or SAP54 plants) show a leafy flower phenotype and result in seedless, suggesting that PHYL1 and SAP54 interfere with reproduction stage that restrict gain-of-function studies in the next generation of transgenic plants. Turnip mosaic virus (TuMV) mild strain (TuGK) has an Arg182Lys mutation in the helper-component proteinase (HC-ProR182K) that blocks suppression of the miRNA pathway and prevents symptom development in TuGK-infected plants. We exploited TuGK as a viral vector for gain-of-function studies of PHYL1 and SAP54 in Arabidopsis plants. TuGK-PHYL1- and TuGK-SAP54-infected Arabidopsis plants produced identical leafy flower phenotypes and similar gene expression profiles as PHYL1 and SAP54 plants. In addition, the leafy flower formation rate was enhanced in TuGK-PHYL1- or TuGK-SAP54-infected Arabidopsis plants that compared with the T0 lines of PHYL1 plants. These results provide more evidence and novel directions for further studying the mechanism of PHYL1/SAP54-mediated leafy flower development. In addition, the TuGK vector is a good alternative in transgenic plant approaches for rapid gene expression in gain-of-function studies. PMID:26076458

  12. GERMINACIÓN DE SEMILLAS DE 32 ESPECIES DE PLANTAS DE LA COSTA DE GUERRERO: SU UTILIDAD PARA LA RESTAURACIÓN ECOLÓGICA

    Microsoft Academic Search

    Héctor Godínez; Arturo Flores-Martínez

    1999-01-01

    We present the results of different germination tests for 32 species of plants that potentially could be used for the recovery of degraded lands in the coast of Guerrero. The experimental treatments consisted in different forms of scarification and thermal stratification to break possible physiological dormancy; the treatments were chosen considering physical and biological characteristics of the seeds. For each

  13. Remote sensing of gene expression in Planta: transgenic plants as monitors of exogenous stress perception in extraterrestrial environments

    NASA Technical Reports Server (NTRS)

    Manak, Michael S.; Paul, Anna-Lisa; Sehnke, Paul C.; Ferl, Robert J.

    2002-01-01

    Transgenic arabidopsis plants containing the alcohol dehydrogenase (Adh) gene promoter fused to the green fluorescent protein (GFP) reporter gene were developed as biological sensors for monitoring physiological responses to unique environments. Plants were monitored in vivo during exposure to hypoxia, high salt, cold, and abcissic acid in experiments designed to characterize the utility and responses of the Adh/GFP biosensors. Plants in the presence of environmental stimuli that induced the Adh promoter responded by expressing GFP, which in turn generated a detectable fluorescent signal. The GFP signal degraded when the inducing stimulus was removed. Digital imaging of the Adh/GFP plants exposed to each of the exogenous stresses demonstrated that the stress-induced gene expression could be followed in real time. The experimental results established the feasibility of using a digital monitoring system for collecting gene expression data in real time from Transgenic Arabidopsis Gene Expression System (TAGES) biosensor plants during space exploration experiments.

  14. In planta production of the highly potent resveratrol analogue pterostilbene via stilbene synthase and O-methyltransferase co-expression

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Stilbenoid phytoalexins such as resveratrol, play an important defense role in several plant species against pathogens such as Botrytis cinerea. In addition to their antimicrobial properties, resveratrol and related stilbenes have also generated considerable interest as nutraceuticals, due to their...

  15. In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA

    PubMed Central

    2010-01-01

    Background Self-complementary RNA transcripts form a double-stranded RNA (dsRNA) that triggers a sequence-specific mRNA degradation, in a process known as RNA interference (RNAi), leading to gene silencing. In vascular plants, RNAi molecules trafficking occur between cells and systemically throughout the plant. RNAi signals can spread systemically throughout a plant, even across graft junctions from transgenic to non-transgenic stocks. There is also a great interest in applying RNAi to pathogenic fungi. Specific inhibition of gene expression by RNAi has been shown to be suitable for a multitude of phytopathogenic filamentous fungi. However, double-stranded (ds)RNA/small interfering (si)RNA silencing effect has not been observed in vivo. Results This study demonstrates for the first time the in vivo interference phenomenon in the pathogenic fungus Fusarium verticillioides, in which expression of an individual fungal transgene was specifically abolished by inoculating mycelial cells in transgenic tobacco plants engineered to express siRNAs from a dsRNA corresponding to the particular transgene. Conclusion The results provide a powerful tool for further studies on molecular plant-microbe and symbiotic interactions. From a biotechnological perspective, silencing of fungal genes by generating siRNAs in the host provides a novel strategy for the development of broad fungi-resistance strategies in plants and other organisms. PMID:20356372

  16. GENÉTICA E MELHORAMENTO DE PLANTAS POTENCIAL DE LINHAGENS DE POPULAÇÕES LOCAIS DE MILHO PIPOCA PARA SÍNTESE DE HÍBRIDOS(1)

    Microsoft Academic Search

    EDUARDO SAWAZAKI; MARIA ELISA A. G. ZAGATTO; JAIRO LOPES DE CASTRO; PAULO BOLLER GALLO; J OÃO; CARLOS CARDOSO; LUIZ ALBERTO SAES

    Médias dos principais caracteres agronômicos de top crosses foram utilizadas para avaliação do potencial de 82 linhagens da variedade de milho pipoca Guarani e 27 da variedade IAC-64 para síntese de híbridos, utilizando-se como testador um híbrido simples de linhagens da variedade South American Mushroom (SAM). Os top crosses foram distribuídos nos experimentos I, II e III, sendo desenvolvidos em

  17. Production and characterization of in planta transiently produced polygalacturanase from Aspergillus niger and its fusions with hydrophobin or ELP tags

    PubMed Central

    2014-01-01

    Background Pectinases play an important role in plant cell wall deconstruction and have potential in diverse industries such as food, wine, animal feed, textile, paper, fuel, and others. The demand for such enzymes is increasing exponentially, as are the efforts to improve their production and to implement their use in several industrial processes. The goal of this study was to examine the potential of producing polygalacturonase I from Aspergillus niger in plants and to investigate the effects of subcellular compartmentalization and protein fusions on its accumulation and activity. Results Polygalacturonase I from Aspergillus niger (AnPGI) was transiently produced in Nicotiana benthamiana by targeting it to five different cellular compartments: apoplast, endoplasmic reticulum (ER), vacuole, chloroplast and cytosol. Accumulation levels of 2.5%, 3.0%, and 1.9% of total soluble protein (TSP) were observed in the apoplast, ER, and vacuole, respectively, and specific activity was significantly higher in vacuole-targeted AnPGI compared to the same enzyme targeted to the ER or apoplast. No accumulation was found for AnPGI when targeted to the chloroplast or cytosol. Analysis of AnPGI fused with elastin-like polypeptide (ELP) revealed a significant increase in the protein accumulation level, especially when targeted to the vacuole where the protein doubles its accumulation to 3.6% of TSP, while the hydrophobin (HFBI) fusion impaired AnPGI accumulation and both tags impaired activity, albeit to different extents. The recombinant protein showed activity against polygalacturonic acid with optimum conditions at pH 5.0 and temperature from 30 to 50°C, depending on its fusion. In vivo analysis of reducing sugar content revealed a higher release of reducing sugars in plant tissue expressing recombinant AnPGI compared to wild type N. benthamiana leaves. Conclusion Our results demonstrate that subcellular compartmentalization of enzymes has an impact on both the target protein accumulation and its activity, especially in the case of proteins that undergo post-translational modifications, and should be taken into consideration when protein production strategies are designed. Using plants to produce heterologous enzymes for the degradation of a key component of the plant cell wall could reduce the cost of biomass pretreatment for the production of cellulosic biofuels. PMID:24970673

  18. Overexpression of the rice carotenoid cleavage dioxygenase 1 gene in Golden Rice endosperm suggests apocarotenoids as substrates in planta

    Microsoft Academic Search

    Andrea Ilg; Qiuju Yu; Patrick Schaub; Peter Beyer; Salim Al-Babili

    2010-01-01

    Carotenoids are converted by carotenoid cleavage dioxygenases that catalyze oxidative cleavage reactions leading to apocarotenoids.\\u000a However, apocarotenoids can also be further truncated by some members of this enzyme family. The plant carotenoid cleavage\\u000a dioxygenase 1 (CCD1) subfamily is known to degrade both carotenoids and apocarotenoids in vitro, leading to different volatile\\u000a compounds. In this study, we investigated the impact of

  19. An in planta-expressed polyketide synthase produces (R)-mellein in the wheat pathogen Parastagonospora nodorum.

    PubMed

    Chooi, Yit-Heng; Krill, Christian; Barrow, Russell A; Chen, Shasha; Trengove, Robert; Oliver, Richard P; Solomon, Peter S

    2015-01-01

    Parastagonospora nodorum is a pathogen of wheat that affects yields globally. Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 (SN477), in P. nodorum that is highly upregulated during infection of wheat leaves. Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as (R)-mellein and (R)-O-methylmellein. Using a Saccharomyces cerevisiae yeast heterologous expression system, we successfully demonstrated that SN477 is the only enzyme required for the production of (R)-mellein. This is the first identification of a fungal PKS that is responsible for the synthesis of (R)-mellein. The P. nodorum ?SN477 mutant did not show any significant difference from the wild-type strain in its virulence against wheat. However, (R)-mellein at 200 ?g/ml inhibited the germination of wheat (Triticum aestivum) and barrel medic (Medicago truncatula) seeds. Comparative sequence analysis identified the presence of mellein synthase (MLNS) homologues in several Dothideomycetes and two sodariomycete genera. Phylogenetic analysis suggests that the MLNSs in fungi and bacteria evolved convergently from fungal and bacterial 6-methylsalicylic acid synthases. PMID:25326302

  20. In planta expression of HIV1 p24 protein using and RNA plant virus-based expression vector

    Microsoft Academic Search

    Guichang Zhang; Carly Leung; Lisa Murdin; Benjamin Rovinski; K. Andrew White

    2000-01-01

    Plant viruses show significant potential as expression vectors for the production of foreign proteins (e.g., antigens) in\\u000a plants. The HIV-1 p24 nucleocapsid protein is an important early marker of HIV infection and has been used as an antigen in\\u000a the development of HIV vaccines. Toward developing a plant-based expression system for the production of p24, we have investigated\\u000a the use

  1. In planta production of the highly potent resveratrol analogue pterostilbene via stilbene synthase and O-methyltransferase co-expression.

    PubMed

    Rimando, Agnes M; Pan, Zhiqiang; Polashock, James J; Dayan, Franck E; Mizuno, Cassia S; Snook, Maurice E; Liu, Chang-Jun; Baerson, Scott R

    2012-04-01

    Resveratrol and related stilbenes are thought to play important roles in defence responses in several plant species and have also generated considerable interest as nutraceuticals owing to their diverse health-promoting properties. Pterostilbene, a 3,5-dimethylether derivative of resveratrol, possesses properties similar to its parent compound and, additionally, exhibits significantly higher fungicidal activity in vitro and superior pharmacokinetic properties in vivo. Recombinant enzyme studies carried out using a previously characterized O-methyltransferase sequence from Sorghum bicolor (SbOMT3) demonstrated its ability to catalyse the A ring-specific 3,5-bis-O-methylation of resveratrol, yielding pterostilbene. A binary vector was constructed for the constitutive co-expression of SbOMT3 with a stilbene synthase sequence from peanut (AhSTS3) and used for the generation of stably transformed tobacco and Arabidopsis plants, resulting in the accumulation of pterostilbene in both species. A reduced floral pigmentation phenotype observed in multiple tobacco transformants was further investigated by reversed-phase HPLC analysis, revealing substantial decreases in both dihydroquercetin-derived flavonoids and phenylpropanoid-conjugated polyamines in pterostilbene-producing SbOMT3/AhSTS3 events. These results demonstrate the potential utility of this strategy for the generation of pterostilbene-producing crops and also underscore the need for the development of additional approaches for minimizing concomitant reductions in key phenylpropanoid-derived metabolites. PMID:21902799

  2. Concentração de potássio na solução nutritiva e a qualidade e número de frutos de melão por planta em hidroponia

    Microsoft Academic Search

    Caciana Cavalcanti Costa; Arthur Bernardes Cecílio Filho; Rodrigo Luiz Cavarianni; José Carlos Barbosa

    2004-01-01

    The experiment was carried out in Jaboticabal-SP. The hybrid Bonus n 0 2 was cropped in randomized blocks design, in split-plot scheme and six replications. Fruit number per plant (2, 3, 4 e free fruit set) and potassium concentration in the nutrient solution (66, 115.5, 165 and 247.5mg L -1) were the studied factors. The increase of potassium concentration in

  3. A report on a comprehensive series of experiments, both in vitro and in planta, to develop treatments for Phytophthora

    E-print Network

    California at Berkeley, University of

    in the U.S. since Dutch Elm Disease. Although it was first observed around the San Francisco Bay Area the cambium of main stems, while on a wide range of other hosts it simply causes leaf spots , leaf blight

  4. PATHOGENICITY AND IN PLANTA MYCOTOXIN ACCUMULATION AMONG MEMBERS OF THE FUSARIUM GRAMINEARUM SPECIES COMPLEX ON WHEAT AND RICE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fusarium head blight (FHB), or scab, is a destructive disease of small grains caused by members of the Fusarium graminearum (Fg) species complex, comprised of at least nine distinct, cryptic species. Members of this complex are known to produce mycotoxins including the trichothecenes deoxynivalenol ...

  5. Effective and specific in planta RNAi in cyst nematodes: expression interference of four parasitism genes reduces parasitic success

    Microsoft Academic Search

    Anoop S. Sindhu; Tom R. Maier; Melissa G. Mitchum; Richard S. Hussey; Eric L. Davis; Thomas J. Baum

    2009-01-01

    Cyst nematodes are highly evolved sedentary plant endoparasites that use parasitism proteins injected through the stylet into host tissues to successfully parasitize plants. These secretory proteins likely are essential for parasitism as they are involved in a variety of parasitic events leading to the establishment of specialized feeding cells required by the nematode to obtain nourishment. With the advent of

  6. ESTIMACIÓN DEL ÁREA FOLIAR EN PLANTAS JÓVENES DE NÍSPERO (Manilkara achras (Miller) Fosberg) SOMETIDAS A DOS AMBIENTES DE LUZ1

    Microsoft Academic Search

    Norkys Meza

    Leaf area determination on sapodilla (Manilkara achras (Miller) Fosberg) young seedlings at two light environments Leaf area was determined for two groups of 15 young seedlings of sapodilla (Manilkara achras (Miller) Fosberg). The first group was located under a lath-house and the second one under a rain-shelter with transparent roof. The length width and leaf area were measured in each

  7. A visual identification key utilizing both gestalt and analytic approaches to identification of Carices present in North America (Plantae, Cyperaceae)

    PubMed Central

    2013-01-01

    Abstract Images are a critical part of the identification process because they enable direct, immediate and relatively unmediated comparisons between a specimen being identified and one or more reference specimens. The Carices Interactive Visual Identification Key (CIVIK) is a novel tool for identification of North American Carex species, the largest vascular plant genus in North America, and two less numerous closely-related genera, Cymophyllus and Kobresia. CIVIK incorporates 1288 high-resolution tiled image sets that allow users to zoom in to view minute structures that are crucial at times for identification in these genera. Morphological data are derived from the earlier Carex Interactive Identification Key (CIIK) which in turn used data from the Flora of North America treatments. In this new iteration, images can be viewed in a grid or histogram format, allowing multiple representations of data. In both formats the images are fully zoomable. PMID:24723777

  8. Development of a Mild Viral Expression System for Gain-Of-Function Study of Phytoplasma Effector In Planta

    PubMed Central

    Liu, Li-Yu Daisy; Hong, Syuan-Fei; Yang, Chiao-Yin; Lo, Hsiao-Feng; Tseng, Ting-Yu; Chen, Wei-Yao; Lin, Shih-Shun

    2015-01-01

    PHYL1 and SAP54 are orthologs of pathogenic effectors of Aster yellow witches’-broom (AYWB) phytoplasma and Peanut witches’-broom (PnWB) phytoplasma, respectively. These effectors cause virescence and phyllody symptoms (hereafter leafy flower) in phytoplasma-infected plants. T0 lines of transgenic Arabidopsis expressing the PHYL1 or SAP54 genes (PHYL1 or SAP54 plants) show a leafy flower phenotype and result in seedless, suggesting that PHYL1 and SAP54 interfere with reproduction stage that restrict gain-of-function studies in the next generation of transgenic plants. Turnip mosaic virus (TuMV) mild strain (TuGK) has an Arg182Lys mutation in the helper-component proteinase (HC-ProR182K) that blocks suppression of the miRNA pathway and prevents symptom development in TuGK-infected plants. We exploited TuGK as a viral vector for gain-of-function studies of PHYL1 and SAP54 in Arabidopsis plants. TuGK-PHYL1- and TuGK-SAP54-infected Arabidopsis plants produced identical leafy flower phenotypes and similar gene expression profiles as PHYL1 and SAP54 plants. In addition, the leafy flower formation rate was enhanced in TuGK-PHYL1- or TuGK-SAP54-infected Arabidopsis plants that compared with the T0 lines of PHYL1 plants. These results provide more evidence and novel directions for further studying the mechanism of PHYL1/SAP54-mediated leafy flower development. In addition, the TuGK vector is a good alternative in transgenic plant approaches for rapid gene expression in gain-of-function studies. PMID:26076458

  9. A straightforward and reliable method for bacterial in planta transcriptomics: application to the Dickeya dadantii/Arabidopsis thaliana pathosystem.

    PubMed

    Chapelle, Emilie; Alunni, Benoît; Malfatti, Pierrette; Solier, Lucie; Pédron, Jacques; Kraepiel, Yvan; Van Gijsegem, Frédérique

    2015-04-01

    Transcriptome analysis of bacterial pathogens is a powerful approach to identify and study the expression patterns of genes during host infection. However, analysis of the early stages of bacterial virulence at the genome scale is lacking with respect to understanding of plant-pathogen interactions and diseases, especially during foliar infection. This is mainly due to both the low ratio of bacterial cells to plant material at the beginning of infection, and the high contamination by chloroplastic material. Here we describe a reliable and straightforward method for bacterial cell purification from infected leaf tissues, effective even if only a small amount of bacteria is present relative to plant material. The efficiency of this method for transcriptomic analysis was validated by analysing the expression profiles of the phytopathogenic enterobacterium Dickeya dadantii, a soft rot disease-causing agent, during the first hours of infection of the model host plant Arabidopsis thaliana. Transcriptome profiles of epiphytic bacteria and bacteria colonizing host tissues were compared, allowing identification of approximately 100 differentially expressed genes. Requiring no specific equipment, cost-friendly and easily transferable to other pathosystems, this method should be of great interest for many other plant-bacteria interaction studies. PMID:25740271

  10. El rastro de Elena Garro: Una mirada feminista

    E-print Network

    Rosas Lopá tegui, Patricia

    2002-04-01

    tesis en cuestión tienen un pie en modelos europeos, tan antiguos como el coro del teatro griego, pero la manera en que Garro los retoca o actualiza para representar asuntos de la sociedad contemporánea, hace que el texto cree su propio modelo teórico...) con su imaginación y su competencia de la tradición retórica y cultural" (125). Inmediatamente se 6 LATIN AMERICAN THEATRE REVIEW observa el predominio en número y jerarquía de los personajes masculinos sobre el único femenino. También se advierte...

  11. INTRODUCCIN El tritn ibrico (Lissotriton boscai) es una espe-

    E-print Network

    Galán, Pedro

    The reproductive biology of Bosca´s newt (Lissotriton boscai) and palmate newt (Lissotriton helveticus) has been correlation between female and eggs size was found in either species. Palmate newts laid more eggs daily than than Bosca´s newt, also being higher the total number of the eggs laid during all the days

  12. Una realizzazione attesa: il catalogo generale delle meridiane in Italia

    Microsoft Academic Search

    Angelo Frosina

    2002-01-01

    The Sundial Section of the UAI has compiled the first catalog of 12643 sundials in 3109 municipalities of Italy. Sundials are instruments that measure the passage of time through the movements of the Sun and the shadows it casts. The volume comprises four chapters with introductoring sections regarding how to approach a reading of volume and an appendix containing additional

  13. Trabajo Investigacin Es la Conexin Itaip Alto Jahuel una locura?

    E-print Network

    Rudnick, Hugh

    .............................................................................. II.4. Relación de Itaipú con Brasil.1. Precio de la energía en Brasil............................................................ III.2

  14. Evoluzione delle velocità di ascensione di una protuberanza solare

    Microsoft Academic Search

    Riccardo Robitschek

    2003-01-01

    CCD images of an eruptive protuberance happened on the Sun the 22nd of March 2002 allow the determination of ascending speeds approaching the gravitational escape velocity. The procedures of image recording, data reduction and results obtained are described.

  15. Collomia linearis Nutt. Una Polemoniacea nordamericana avventizia nel Bellunese

    Microsoft Academic Search

    Erika Pignatti Wikus

    1973-01-01

    Collomia linearis Nutt. (Polemoniaceae) naturalized near Belluno, Northern Italy. – Collomia linearis Nutt., a North American Poleomoniacea, has been found naturalized in the gravelly stream-bed of the Pettorina, near Rocca Pietore (province of Belluno), at about 1200 m on s.l. The Pettorina is a seasonal stream and quite without water during the main part of the year. Most of its

  16. Derechos humanos, una oportunidad para las políticas públicas en salud

    Microsoft Academic Search

    Álvaro Franco-Giraldo; Carlos Álvarez-Dardet

    2008-01-01

    Human rights outlined a better scenario for public policies in health. For it requires intersectoral and interdisciplinary approach. This article emphasizes the perspective of public health policies based on human rights, clarifies the relationship of public policies with the exercise of human rights, beyond the right to health. It recognizes the need to implement genuinely democratic and participatory mechanisms.It considers

  17. Los derechos de los pacientes: una cuestión de calidad

    Microsoft Academic Search

    Pablo Hernando

    2005-01-01

    Patients’ rights first arose as a question of quality from the perspective of users themselves in the United States, a cultural environment that differs from our own. This contextual difference led to difficulties in implementing and understanding patients’ rights in Spain.Patients’ rights are the specification of human rights in the management of the patient's own body and are one more

  18. Los felinos: ¿Una alternativa en estudios de toxicología genética?

    Microsoft Academic Search

    Ana Zamora-Perez; Belinda C. Gómez-Meda; María L. Ramos-Ibarra; Cecilia M. Batista; Jaime Luna-Aguirre; Andrés González-Rodríguez; José L. Rodríguez-Ávila; Guillermo M. Zúñiga-González; Medio Ambiente

    2008-01-01

    Felines: an alternative in genetic toxicology studies? The micronuclei (MN) test carry out in peripheral blood is fast, simple, economic and it is used to detect genotoxic environmental agents. MN are frag- ments of chromosomes or complete chromosomes remaining in the cytoplasm after cell division, which increase when organisms are exposed to genotoxic agents. Therefore, species with the highest values

  19. Responsabilidad Social Empresarial: Una opción de cambio impostergable

    Microsoft Academic Search

    Rodeloy Castellanos Cruz

    2007-01-01

    En este trabajo de responde a un conjunto de cuestiones asociadas a la Responsabilidad Social Empresarial (RSE) que constituyen en sí puntos comunes de encuentro y desencuentro de criterios. ¿Cuál es el rol del Estado a la hora de crear un ambiente propicio a la RSE? ¿Hasta dónde están las empresas obligadas a actuar en beneficio de la sociedad? ¿Tiene

  20. Responsabilidad social y buen clima laboral: Una fórmula ganadora

    Microsoft Academic Search

    Elsa del Castillo; Gustavo Yamada

    2008-01-01

    El presente estudio, financiado por la Cátedra del Banco Santander Central Hispano de Responsabilidad Social Empresarial 2006-2007, evalúa las tendencias recientes en la dimensión laboral de la responsabilidad social empresarial en compañías líderes en el Perú. Su objetivo es analizar y difundir las mejores prácticas en la gestión del equipo humano, documentar los beneficios que éstas representan a las empresas

  1. Una Perspectiva de Mexico: Its People, Places and History.

    ERIC Educational Resources Information Center

    Bianchi, Gary; Bianchi, Maria Eugenia Matute

    Evolving from a general commitment to the goals of cultural pluralism and bicultural education, this portfolio of 24 full color and halftone photographs aims to reinforce those curricular objectives which emphasize a respect for the value and individuality of different cultures and groups, affirm the right of an individual to maintain a bicultural…

  2. Tratamiento individualizado de la EPOC: una propuesta de cambio

    Microsoft Academic Search

    Marc Miravitlles

    2009-01-01

    Chronic obstructive pulmonary disease (COPD) is a highly prevalent but poorly defined disease and there is wide confusion regarding its treatment. The available knowledge should allow guidelines for specific treatment to be established according to each patient's clinical features. The present article describes a proposal for individually-tailored treatment. Long-acting bronchodilators, alone or in various combinations, would be the basis of

  3. BIOÉTICA PARA UNA SALUD PÚBLICA CON RESPONSABILIDAD SOCIAL

    Microsoft Academic Search

    María Iliana Ortíz; Susana Palavecino

    2005-01-01

    Is it possible for economy sciences to offer a bioethics' contribution for Public Health? Our aim was to establish a dialogue with economic trends developed during the last decades and to see how and up to what extent they can contribute to Public Health within the Latinamerican context. For this purpose we individualized some social responsibility indicators undertaken by enterprises

  4. ¿CÓMO SABER QUE TENEMOS UNA GESTIÓN DE PROYECTOS EFECTIVA?

    Microsoft Academic Search

    Esperanza Aguillón Robles; Lourdes García Ávila; Ruth Vargas Rivera; José Cuitláhuac Guzmán Hdez; Luis Calderón Aguirre

    2008-01-01

    El sector de las PyMes es uno de los sectores de crecimiento económico más importante para Latinoamérica, y la tendencia mundial hacia la globalización y la erradicación de las fronteras geográficas, ha empezado a obligar a este sector a crear nuevas estrategias de competencia en un entorno cada vez más cambiante, competitivo y exigente. En este orden, los gobiernos vienen

  5. Cómo hacer promociones vendedoras siendo una pequeña o mediana empresa

    Microsoft Academic Search

    Carlos Alberto Areiza

    2000-01-01

    En los mercados tan altamente competidos que se están dando actualmente se hace necesario que el comercializador promocione sus productos más frecuentemente, con promociones mejor diseñadas y pensando en el cliente. Las promociones casi siempre han estado relacionadas con «regalar algo»; cuando a un productor o un comerciante le hablan de promoción por lo general piensa en «pague 2 y

  6. Termodinamica del universo temprano desde una cosmolog\\`Ia newtoniana

    E-print Network

    C, W A Rojas

    2011-01-01

    We study a model of fluent material universe from a modified Newtonian cosmology proposed by D'Inverno and Tawfik. From this perspective, it shows a list of space-time curvature k with energy E, density \\rho, specific heat $C_{p}$ and the fluid temperature gradient considered $\\Delta T$ corresponding to standard thermodynamic variables. We determined the coupling constants accompanying the matter density for different values we can take k

  7. Termodinamica del universo temprano desde una cosmologÌa newtoniana

    E-print Network

    W. A. Rojas C

    2011-10-18

    We study a model of fluent material universe from a modified Newtonian cosmology proposed by D'Inverno and Tawfik. From this perspective, it shows a list of space-time curvature k with energy E, density \\rho, specific heat $C_{p}$ and the fluid temperature gradient considered $\\Delta T$ corresponding to standard thermodynamic variables. We determined the coupling constants accompanying the matter density for different values we can take k

  8. IA chiariflocculazione Sommario In questo lavoro si presenta una metodolo-

    E-print Network

    minimizzare il suo effetto sul- l'acqua trattata. DIAGNOSIS OF FLOCCULATION PROCESS THROUGH ARTIFICIAL mode of operation. In this way the algorithm can supply the supervisor with enough information to take of a severe and sudden turbidity increase showing how the algorithm can trace back the causes and propose

  9. Fragmentos de Sombra: Una Biografía Intelectual de Fernando González

    E-print Network

    Palacios Perez, Jose

    2014-07-01

    González (1895 –1964) sigue siendo leída en cafés y tertulias, y de que su legado cultural está más vivo que nunca, las aproximaciones académicas a su obra son pocas y se limitan a apenas algunas de sus obras. Este trabajo pretende llenar ese vacío. El.... El segundo ruta busca un puente entre los métodos académicos y los lectores de Fernando González más allá de la academia. El recorrido de estos dos caminos desnudó el pensamiento fragmentario como base fundamental del tejido de la realidad para...

  10. Chile vivienda incremental: Una investigación del Banco Interamericano de Desarrollo

    Microsoft Academic Search

    Margarita Greene; Enrique González

    2012-01-01

    El presente informe responde al estudio Self-Help Housing and Incremental Housing: The Likely Directions for Future Housing Policy financiado por el Banco Interamericano de Desarrollo donde se tomó como caso de estudio la experiencia chilena de la vivienda incremental. El objetivo del estudio es aprender de la experiencia realizada para desde ahí ofrecer opciones estratégicas a los gobiernos que apoyen

  11. 4-graficas tensas y una probadita de topologia Ricardo Strausz*

    E-print Network

    Strausz, Ricardo

    ´ales superficies la puedo dibujar `bien'? Es sabido desde el trabajo de Kuratowsky [1] que la gr´afica completa de cinco v´ertices K5 no se puede dibujar en el plano al igual que la gr´afica completa bipartita K3,3, y

  12. App. Android Accesible para una Rutina de Movilidad Articular y

    E-print Network

    Boemo, Eduardo

    de parámetros como la actividad física, la edad, el género, o lesiones anteriores. #12;Movilidad Articular y Espalda Objetivos · Facilidad para realizar tareas. · Menor gasto de energía. · Reducción del

  13. La Ciencia de los Antiguos Mexicanos: Una Bibliografia Selecta

    ERIC Educational Resources Information Center

    Ortiz-Franco, Luis; Magana, Maria

    1973-01-01

    Fifty-five citations pertaining to the scientific and mathematic development of ancient Mexicans, particularly the Mayas, are given in this select bibliography. The introduction and descriptions of resource libraries in 8 States are in Spanish. (NQ)

  14. CONTROL BIOLÓGICO: UNA HERRAMIENTA PARA EL DESARROLLO SUSTENTABLE Y SOSTENIBLE. \\

    Microsoft Academic Search

    Clemencia Guédez; Carmen Castillo; Luis Cañizales; Rafael Olivar; Adolfo Navas Coronado

    2008-01-01

    The sustainable development is referred to the efficient and rational administration of our natural resources, with the objective of improving the current welfare population, without committing the quality of future generations life. The protection of the atmosphere and the sustainable human development go together, and it is necessary to enlarge the investigations on the use of biological methods for cultivations

  15. Comorbilidad entre trastorno bipolar y migraña: una revisión sistemática

    Microsoft Academic Search

    Germán Eduardo; Rueda Jaimes; Iván Augusto; Gaona Barbosa; Yuli Andrea Martínez Ladino

    2005-01-01

    Introduction: Migraine and bipolar disorder (BD) are prevalent conditions and of great relevance for the general population. Some studies have informed of their association but until now we don't know if it is real. Objective: To conduct a systematic review to evaluate the known evidence of the association between migraine and BD. Methods: A search was placed in Medline, Proquest

  16. Gaultheria nubigena (Ericaceae), una especie rara en la Argentina

    Microsoft Academic Search

    PAULA M. HERMANN; VIVIANA N. CAMBI

    Summary: Gaultheria nubigena (Ericaceae), a rare species in Argentina. During approximately 20 years of collecting material for floral development studies of Ericaceae in the subantarctic forests of Argentina, eight species of the nine that occur there were easily found. The exception was G. nubigena. It is a rare species; fifty-two years have elapsed since its collection in 1952 and more

  17. Una breve gua para padres acerca del autismo: Informacin para

    E-print Network

    Sarkar, Nilanjan

    Tennessee y que recientemente haya recibido un diagnóstico de trastorno del espectro autista (ASD por sus o trastorno generalizado del desarrollo-no especificado (PDD-NOS por sus siglas en inglés). Incluso si usted ha estado sospechando un trastorno del espectro autista, recibir el diagnóstico puede

  18. Responsabilidad social corporativa. Una visión desde la teoría económica

    Microsoft Academic Search

    Aleix Calveras; Juan José Ganuza

    2004-01-01

    El objetivo de este trabajo es discutir y analizar el concepto de la responsabilidad social corporativa (RSC) desde la teoria económica. Para ello usamos un modelo sencillo que nos permite clarificar algunos aspectos del debate que rodea la RSC. Observamos como la motivación subyacente en las decisiones y estrategias empresariales juega un papel importante en el concepto de RSC; así,

  19. UNA REVISIÓN BÁSICA SOBRE CONCEPTOS Y TEORÍAS DE GOBERNABILIDAD

    Microsoft Academic Search

    Xiomara Araujo

    Governance is a concept widely used to explain governing processes, particularly those undertaken by nation states as an answer to its external environments, or those actions that refer the role of the state in co-ordinating the interaction between the public and the private sector. This is also a concept linked to the debates on development and democracy. There are several

  20. Educacion y crecimiento: una perspectiva mundial 1960-90

    Microsoft Academic Search

    Isabel Neira

    1997-01-01

    En este trabajo estudiamos el papel del capital humano como uno de los factores determinantes del crecimiento economico de un pais, para ello consideramos dos variables fundamentales, el nivel educativo alcanzado por la poblacion activa y el gasto en educacion como medida de la calidad de la enseñanza. Se realiza un estudio comparativo, para 118 paises, de ambas variables en

  1. UNA HERRAMIENTA DE MEJORA, EL OEE (EFECTIVIDAD GLOBAL DEL EQUIPO)

    Microsoft Academic Search

    Hugo Leonel Alonzo González

    2009-01-01

    En el presente artículo se definen los conceptos de Efectividad Global del Equipamiento (OEE) como herramienta de mejora continua, enmarcado en la industria manufacturera actual y su relación con el Mantenimiento Productivo Total (TPM), y como el mismo mide a diferencia de otros indicadores en un solo Ratio el porcentaje de efectividad de las máquinas y líneas con respecto a

  2. Experiencias y repercusión de una formación en ética de investigación

    PubMed Central

    Rupaya, Carmen Rosa García

    2012-01-01

    El presente artículo tiene como propósito describir los logros y repercusiones de la capacitación en ética de la investigación que brinda el Centro Interdisciplinario de Estudios en Bioética de la Universidad de Chile, sirviendo de estímulo, motivación y orientación a profesionales que requieren conocer y aplicar las normas y el raciocinio conducente a la deliberación de los problemas en esta disciplina. Asimismo, describe cómo este conocimiento genera un efecto multiplicador en aspectos tales como la participación en un comité de ética de la investigación (CEI), organización de cursos y creación y desarrollo de líneas de investigación, que repercuten en publicaciones realizadas con estudiantes de posgrado. Relata además los contenidos y estrategias didácticas que pueden ser empleados en cursos de ética y bioética para estudiantes de estomatología y concluye mencionando la aplicación práctica de esta capacitación en los ámbitos docente, institucional y de investigación. PMID:24482556

  3. Actuación en una inversión uterina. Estudio de un caso

    Microsoft Academic Search

    Beatriz Tosal; Ramón Pastor

    Title: Management of inversion of the uterus: a case report Inversion of the uterus is a very serious complication of the third stage of labor. Its proper management and, above all, early diagnosis by the midwife help to reduce the incidence of the complications of this obstetric emergency.

  4. Fibromatosis de mama: una lesión simuladora de cáncer

    Microsoft Academic Search

    Baltazar Alberto Soto-Dávalos; Juan Enrique Bargalló-Rocha; Artfy Bandera-Delgado; Oscar Zumaran-Cuéllar; Carlos Daniel Robles-Vidal

    2008-01-01

    Summary Background: Breast fibromatosis (BF) is a rare benign pathological entity. Its etiology is unknown, but it has been associated with surgical trauma and certain genetic disorders. Clinical cases: Case 1. The patient was a 17-year-old female with a 20 × 15 cm firm and fixed mass in the right breast. A core- needle biopsy was taken with a pathology

  5. Lupus eritematoso túmido, una entidad en proceso de definición

    Microsoft Academic Search

    C. Rodríguez-Caruncho; I. Bielsa

    2011-01-01

    Lupus erythematosus tumidus (LET), a form of cutaneous lupus erythematosus that was described some years ago, has begun to receive more attention in the past decade as many published studies have helped to define the particularities of this subtype.The clinical features of LET include the absence of changes on the surface of the epidermis (such as erosion, atrophy, scaling, or

  6. InteligenciaNet: Una Experiencia de Innovación Educativa

    Microsoft Academic Search

    MariCarmen González Videgaray; Rubén Romero Ruiz; Río Martínez

    Currently some teachers are developing instructional materials to be used in computer by students. These products can be distributed in compact disks, local net or Internet. However, their value would be greater with the possibility of knowing if the content reached the students effectively. That is precisely the function of virtual learning environments or learning management systems. The aim of

  7. Esercitazioni Matematiche: Una Rivista Ad uso Degli Studenti Universitari.

    ERIC Educational Resources Information Center

    Micale, Biagio

    1992-01-01

    The history of the journal "Esercitazioni Matematiche" (1921-43) edited by the "Circolo Matematico di Catania" for university students is described. A selection of proposed problems with some of their solutions is reported. (Author)

  8. Fragmentos de Sombra: Una Biografía Intelectual de Fernando González 

    E-print Network

    Palacios Perez, Jose

    2014-07-01

    de Santiago Pérez, director del Partido Liberal, de garantías electorales, libertad de prensa, supresión de los monopolios fiscales y del Banco de la República. Sin embargo hacia 1984 la línea dura en contra de los liberales se recrudeció y Pérez fue...

  9. El seminario consta de dos partes: Una primera parte (terica) sobre la Optimizacin delTDA-H desde una

    E-print Network

    Escolano, Francisco

    : · AnalizarlarealidaddelossujetosconTDAH,desdesuprimera infancia hasta la entrada en la edad adultaTDAH. · Promovercompetenciasenelalumnadoquelespermitadesarrollar las aptitudes de los menores conTDAH,para desenvolverse mejor por las diferentes trayectorias

  10. Use of medicinal plants among patients with diabetes mellitus type 2 in Morelos, Mexico (Uso de plantas medicinales por pacientes con diabetes mellitus tipo 2 en Morelos, México)

    Microsoft Academic Search

    Ofelia ROMERO-CERECERO; Hortensia REYES-MORALES; Lucía AGUILAR-SANTAMARÍA; Maira HUERTA-REYES; Jaime TORTORIELLO-GARCIA

    The aim of the study was the identification of the most frequently used plants by patients with DM2 with access to public health services in Morelos, Mexico. A questionnaire was employed with 259 patients with DM2 who regularly utilize medicinal plants as a complementary therapy, and another 259 patients who only use the pharmaceutical prescription in primary-care clinics from two

  11. Taxonomic Treatment of Solanum Section Petota (Wild Potatoes) in Catálogo de Plantas Vasculares del Cono Sur (Argentina, Chile, Paraguay, Uruguay, y sur del Brasil)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Solanum section Petota (Solanaceae), which includes the cultivated potato (Solanum tuberosum) and its wild relatives, contains over 150 wild species distributed from the southwestern U.S.A. (38°N) to central Argentina and adjacent Chile (41°S). This catalog includes all species from the Southern Con...

  12. APROXBMACI6N A LA BIBLIOGRAFIA FLORISTICA BASICA DE PLANTAS VASCULARES DE LA PENINSULA IBERICA EISLAS BALEARES, II: 1989-1998

    E-print Network

    Moreno Saiz, Juan Carlos

    vasculares de la Peninsula Iberica e Islas Baleares, II: 1989-1998. Armies Jard Bot. Madrid 57(2): 341 and Balearic Islands, II: 1989- 1998. Armies Jard. Bot. Madrid 51(2): 341-356 (in Spanish). A new relation

  13. Interaction of VPg-Pro of Turnip mosaic virus with the translation initiation factor 4E and the poly(A)-binding protein in planta

    Microsoft Academic Search

    Simon Leonard; Catherine Viel; Chantal Beauchemin; Nicole Daigneault; Marc G. Fortin; Jean-Francois Laliberte

    2004-01-01

    The viral protein linked to the genome (VPg) of Turnip mosaic virus (TuMV) interacts in vitro with the translation eukaryotic initiation factor (eIF) 4E. In the present study, we investigated the consequence of TuMV infection on eIF4E expression. Two isomers are present in plants, namely eIF4E and eIF(iso)4E. Expression of the latter was detected in both TuMV-infected and mock-inoculated Brassica

  14. Analysis of RNA-Mediated Gene Silencing Using a New Vector (pKNOCKOUT) and an In Planta Agrobacterium Transient Expression System

    Microsoft Academic Search

    CHRISTOPHER IAN CAZZONELLI; JEFF VELTEN

    2005-01-01

    A hairpin RNA (hpRNA) vector, pKNOCKOUT (pKO) has been constructed to facilitate the analysis of posttranscriptional gene silencing (PTGS) in an Agrobacterium- mediated transient expression system developed for tobacco. The pKO binary vector was tested by cloning a firefly luciferase (Photinus pyralis) gene segment in sense (sFLUC), antisense (aFLUC), and inverted repeat (ihpFLUC) orientations. The inverted repeats of the target

  15. Transient co-expression of post-transcriptional gene silencing suppressors and ?-glucuronidase in harvested lettuce leaf tissue does not improve recombinant protein accumulation in planta

    Microsoft Academic Search

    Christopher W. Simmons; Jean S. VanderGheynst

    2007-01-01

    Agrobacterium-mediated gene transfer was used to co-express three virus-derived post-transcriptional gene silencing (PTGS) suppressors,\\u000a P19 from tomato bushy stunt virus and two species of helper component proteinase (HcPro) from tobacco etch virus (TEV) and\\u000a turnip mosaic virus, with ?-glucuronidase (GUS) in harvested lettuce leaf tissue to investigate whether GUS accumulation increases\\u000a in the presence of PTGS suppressors. Co-expression incubations were

  16. Analysis of RNA-mediated gene silencing using a new vector (pKNOCKOUT) and an in planta Agrobacterium transient expression system

    Microsoft Academic Search

    Christopher Ian Cazzonelli; Jeff Velten

    2004-01-01

    A hairpin RNA (hpRNA) vector, pKNOCKOUT (pKO) has been constructed to facilitate the analysis of posttranscriptional gene\\u000a silencing (PTGS) in anAgrobacterium-mediated transient expression system developed for tobacco. The pKO binary vector was tested by cloning a firefly luciferase\\u000a (Photinus pyralis) gene segment in sense (sFLUC), antisense (aFLUC), and inverted repeat (ihpFLUC) orientations. The inverted repeats of the\\u000a target gene are

  17. Pea lectin receptor-like kinase promotes high salinity stress tolerance in bacteria and expresses in response to stress in planta

    Microsoft Academic Search

    Amita Joshi; Hung Quang Dang; Neha Vaid; Narendra Tuteja

    2010-01-01

    The plant lectin receptor-like kinases (LecRLKs) are involved in various signaling pathways but their role in salinity stress\\u000a tolerance has not heretofore been well described. Salinity stress negatively affects plant growth\\/productivity and threatens\\u000a food security worldwide. Based on functional gene-mining assay, we have isolated 34 salinity tolerant genes out of one million\\u000a Escherichia coli (SOLR) transformants containing pea cDNAs grown

  18. A semi-quantitative RT-PCR method to readily compare expression levels within Botrytis cinerea multigenic families in vitro and in planta

    Microsoft Academic Search

    Mathias Choquer; Martine Boccara; Anne Vidal-Cros

    2003-01-01

    A straightforward and easy-to-apply semi-quantitative RT-PCR method was developed to study multigenic expression in the phytopathogenic fungus Botrytis cinerea. This procedure is based on the one-step reverse transcription-amplification of a specific transcript within total RNA and product amount determination by densitometric analysis of ethidium bromide fluorescence upon gel electrophoresis. The semi-quantitative analysis is achieved, at a fixed PCR cycle-number, within

  19. INCIDENCE, AGRESSIVENESS AND IN PLANTA INTERACTIONS OF BOTRYTIS CINEREA ANDOTHER FILAMENTOUS FUNGI QUIESCENT IN GRAPE BERRIES AND DORMANT BUDS IN CENTRAL WASHINGTON STATE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Recovery of quiescent filamentous fungi from non-symptomatic grape berries and dormant buds demonstrated dominance of Alternaria, Aureobasidium, Cladosporium, Ulocladium and other dematiaceous hyphomycetes. Up to 78% of berries contained fungi prior to harvest. Botrytis cinerea was recovered from 0...

  20. Plaza de las Cortes, 2-7 planta. 28014 Madrid Tel: +34 91 360 12 00 se.cadep@crue.org V2.0.05/09/11

    E-print Network

    Fraguela, Basilio B.

    Conferencia de Rectores de las Universidades Españolas de la Calidad Ambiental, Desarrollo Sostenible y la y Química Agrícola y coordinadora del Plan de Desarrollo Sostenible de la Universidad de Santiago de Excoordinadora del Plan de Desarrollo Sostenible de la Universidad de Santiago de Compostela. Noelia López

  1. In planta production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus X

    Microsoft Academic Search

    Gianpiero Marconi; Emidio Albertini; Pierluigi Barone; Francesca De Marchis; Chiara Lico; Carla Marusic; Domenico Rutili; Fabio Veronesi; Andrea Porceddu

    2006-01-01

    BACKGROUND: Classical Swine Fever (CSFV) is one of the most important viral infectious diseases affecting wild boars and domestic pigs. The etiological agent of the disease is the CSF virus, a single stranded RNA virus belonging to the family Flaviviridae. All preventive measures in domestic pigs have been focused in interrupting the chain of infection and in avoiding the spread

  2. In planta processing and glycosylation of a nematode CLE effector and its interaction with a CLV2-like receptor to promote parasitism

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Like other biotrophic plant pathogens, plant-parasitic nematodes secrete effector proteins into host cells to facilitate infection. Effector proteins that mimic plant CLAVATA3/ESR (CLE)-like proteins have been identified in several cyst nematodes including the potato cyst nematode (PCN); however, th...

  3. Phylogenetic Analysis of Downy Mildew Pathogens of Opium Poppy and PCR-Based In Planta and Seed Detection of Peronospora arborescens.

    PubMed

    Landa, Blanca B; Montes-Borrego, Miguel; Muñoz-Ledesma, Francisco J; Jiménez-Díaz, Rafael M

    2007-11-01

    ABSTRACT Severe downy mildew diseases of opium poppy (Papaver somniferum) can be caused by Peronospora arborescens and P. cristata, but differentiating between the two pathogens is difficult because they share morphological features and a similar host range. In Spain, where severe epidemics of downy mildew of opium poppy have occurred recently, the pathogen was identified as P. arborescens on the basis of morphological traits. In this current study, sequence homology and phylogenetic analyses of the internal transcribed spacer regions (ITS) of the ribosomal DNA (rDNA) were carried out with DNA from P. arborescens and P. cristata from diverse geographic origins, which suggested that only P. arborescens occurs in cultivated Papaver somniferum in Spain. Moreover, analyses of the rDNA ITS region from 27 samples of downy-mildew-affected tissues from all opium-poppy-growing regions in Spain showed that genetic diversity exists within P. arborescens populations in Spain and that these are phylogenetically distinct from P. cristata. P. cristata instead shares a more recent, common ancestor with a range of Peronospora species that includes those found on host plants that are not members of the Papaveraceae. Species-specific primers and a PCR assay protocol were developed that differentiated P. arborescens and P. cristata and proved useful for the detection of P. arborescens in symptomatic and asymptomatic opium poppy plant parts. Use of these primers demonstrated that P. arborescens can be transmitted in seeds and that commercial seed stocks collected from crops with high incidence of the disease were frequently infected. Field experiments conducted in microplots free from P. arborescens using seed stocks harvested from infected capsules further demonstrated that transmission from seedborne P. arborescens to opium poppy plants can occur. Therefore, the specific-PCR detection protocol developed in this study can be of use for epidemiological studies and diagnosing the pathogen in commercial seed stocks; thus facilitating the sanitary control of the disease and avoidance of the pathogen distribution in seeds. PMID:18943506

  4. Time-Resolved Fluorescence Imaging Reveals Differential Interactions of N-Glycan Processing Enzymes across the Golgi Stack in Planta1[W][OA

    PubMed Central

    Schoberer, Jennifer; Liebminger, Eva; Botchway, Stanley W.; Strasser, Richard; Hawes, Chris

    2013-01-01

    N-Glycan processing is one of the most important cellular protein modifications in plants and as such is essential for plant development and defense mechanisms. The accuracy of Golgi-located processing steps is governed by the strict intra-Golgi localization of sequentially acting glycosidases and glycosyltransferases. Their differential distribution goes hand in hand with the compartmentalization of the Golgi stack into cis-, medial-, and trans-cisternae, which separate early from late processing steps. The mechanisms that direct differential enzyme concentration are still unknown, but the formation of multienzyme complexes is considered a feasible Golgi protein localization strategy. In this study, we used two-photon excitation-Förster resonance energy transfer-fluorescence lifetime imaging microscopy to determine the interaction of N-glycan processing enzymes with differential intra-Golgi locations. Following the coexpression of fluorescent protein-tagged amino-terminal Golgi-targeting sequences (cytoplasmic-transmembrane-stem [CTS] region) of enzyme pairs in leaves of tobacco (Nicotiana spp.), we observed that all tested cis- and medial-Golgi enzymes, namely Arabidopsis (Arabidopsis thaliana) Golgi ?-mannosidase I, Nicotiana tabacum ?1,2-N-acetylglucosaminyltransferase I, Arabidopsis Golgi ?-mannosidase II (GMII), and Arabidopsis ?1,2-xylosyltransferase, form homodimers and heterodimers, whereas among the late-acting enzymes Arabidopsis ?1,3-galactosyltransferase1 (GALT1), Arabidopsis ?1,4-fucosyltransferase, and Rattus norvegicus ?2,6-sialyltransferase (a nonplant Golgi marker), only GALT1 and medial-Golgi GMII were found to form a heterodimer. Furthermore, the efficiency of energy transfer indicating the formation of interactions decreased considerably in a cis-to-trans fashion. The comparative fluorescence lifetime imaging of several full-length cis- and medial-Golgi enzymes and their respective catalytic domain-deleted CTS clones further suggested that the formation of protein-protein interactions can occur through their amino-terminal CTS region. PMID:23400704

  5. Vascular Streak Dieback of cacao in Southeast Asia detection and Melanesia: in planta detection of the pathogen and a new taxonomy

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vascular Streak Dieback (VSD) disease of cacao (Theobroma cacao) in Southeast Asia and Melanesia is caused by a basidiomycete (Ceratobasidiales) fungus described in a monotypic genus as Oncobasidium theobromae (syn. =Thanatephorus theobromae). The symptoms of the disease include green-spotted chloro...

  6. Characterization of bacterial isolates from rotting potato tuber tissue showing antagonism to Dickeya sp. biovar 3 in vitro and in planta

    Microsoft Academic Search

    R. L. Czajkowski; Boer de W. J; Veen van J. A; Wolf van der J. M

    2012-01-01

    Possibilities for biocontrol of biovar 3 Dickeya sp. in potato were investigated, using bacteria from rotting potato tissue isolated by dilution plating on nonselective agar media. In a plate assay, 649 isolates were screened for antibiosis against Dickeya sp. IPO2222 and for the production of siderophores. Forty-one isolates (6Æ4%) produced antibiotics and 112 isolates (17Æ3%) produced siderophores. A selection of

  7. Characterization of bacterial isolates from rotting potato tuber tissue showing antagonism to Dickeya sp. biovar 3 in vitro and in planta

    Microsoft Academic Search

    R. L. Czajkowski; W. J. De Boer; J. A. Van Veen; J. M. Van der Wolf

    2012-01-01

    Possibilities for biocontrol of biovar 3 Dickeya sp. in potato were investigated, using bacteria from rotting potato tissue isolated by dilution plating on nonselective agar media. In a plate assay, 649 isolates were screened for antibiosis against Dickeya sp. IPO2222 and for the production of siderophores. Forty-one isolates (6·4%) produced antibiotics and 112 isolates (17·3%) produced siderophores. A selection of

  8. In Planta Recognition of a Double-Stranded RNA Synthesis Protein Complex by a Potexviral RNA Silencing Suppressor[C][W][OPEN

    PubMed Central

    Okano, Yukari; Senshu, Hiroko; Hashimoto, Masayoshi; Neriya, Yutaro; Netsu, Osamu; Minato, Nami; Yoshida, Tetsuya; Maejima, Kensaku; Oshima, Kenro; Komatsu, Ken; Yamaji, Yasuyuki; Namba, Shigetou

    2014-01-01

    RNA silencing plays an important antiviral role in plants and invertebrates. To counteract antiviral RNA silencing, most plant viruses have evolved viral suppressors of RNA silencing (VSRs). TRIPLE GENE BLOCK PROTEIN1 (TGBp1) of potexviruses is a well-characterized VSR, but the detailed mechanism by which it suppresses RNA silencing remains unclear. We demonstrate that transgenic expression of TGBp1 of plantago asiatica mosaic virus (PlAMV) induced developmental abnormalities in Arabidopsis thaliana similar to those observed in mutants of SUPPRESSOR OF GENE SILENCING3 (SGS3) and RNA-DEPENDENT RNA POLYMERASE6 (RDR6) required for the trans-acting small interfering RNA synthesis pathway. PlAMV-TGBp1 inhibits SGS3/RDR6-dependent double-stranded RNA synthesis in the trans-acting small interfering RNA pathway. TGBp1 interacts with SGS3 and RDR6 and coaggregates with SGS3/RDR6 bodies, which are normally dispersed in the cytoplasm. In addition, TGBp1 forms homooligomers, whose formation coincides with TGBp1 aggregation with SGS3/RDR6 bodies. These results reveal the detailed molecular function of TGBp1 as a VSR and shed new light on the SGS3/RDR6-dependent double-stranded RNA synthesis pathway as another general target of VSRs. PMID:24879427

  9. Why Range Forage Quality Changes (Spanish) 

    E-print Network

    Lyons, Robert K.; Machen, Richard V.; Forbes, T. D. A.

    2001-09-10

    principalmente en herb?ceas y en hojas de plantas le?osas, puede tener algunos beneficios nutricionales. Por ejemp- lo, niveles bajos de taninos aparentemente dis- minuyen la degradaci?n de las prote?nas por los microorganismos del rumen. Cuando esta prote?- na... tanto beneficios como retos. El beneficio de esta diver- sidad es que la calidad del forraje puede poten- cialmente mantenerse por per?odos de tiempo m?s largos que con una sola especie. Desde el punto de vista de la producci?n animal, uno de los retos...

  10. Incidental Vocabulary Learning in Second Language Acquisition: A Literature Review (Aprendizaje incidental de vocabulario en la adquisición de una segunda lengua: una revisión de literatura)

    ERIC Educational Resources Information Center

    Restrepo Ramos, Falcon Dario

    2015-01-01

    This literature review aims to analyze previous studies that address the incidental learning of vocabulary in second language acquisition. The articles included in this literature review look into the understanding of vocabulary learning through incidental means, the relationship of reading and incidental vocabulary learning, and the strategies…

  11. Aplicaciones Lineales Primeras definiciones. Una aplicacion lineal de un K-ev de salida E a un K-ev de llegada F es una

    E-print Network

    Ramírez-Ros, Rafael

    referentes al n´ucleo y a la imagen no requieren la hip´otesis de dimensi´on finita. Ejercicio. Probar estas con los ejemplos m´as sencillos posibles: La aplicaci´on nula de un ev E a otro ev F es aquella que

  12. VALUTAZIONE DELL'INFLUENZA DEL DIAMETRO DELLE PULEGGE DI PROVA SULLA DURATA A FATICA DI UNA CORDICELLA CON UNA NUOVA ATTREZZATURA DI PROVA

    Microsoft Academic Search

    R. Ciuffi; F. Piccioli

    The paper deals with the considerations which lead to the design and construction of a dedicated rig to perform traction-flexion fatigue tests on small wire ropes. This rig has been used to explore the influence of the diameter of the rollers (pulleys) on the fatigue life of the tested wire rope. The results of the tests to ascertain the type

  13. El seminario consta de dos partes independientes: Una primera parte o Seminario terico con el ttulo Comprendiendo elTDA-H y una segunda

    E-print Network

    Escolano, Francisco

    : · AnalizarlarealidaddelossujetosconTDAH,desdesuprimera infancia hasta la entrada en la edad adultaTDAH. ·Promovercompetenciasenelalumnadoquelespermitadesarrollar las aptitudes de los menores conTDAH, para desenvolverse mejor por las diferentes trayectorias

  14. Peer Interaction: A Social Perspective towards the Development of Foreign Language Learning (Interacción entre pares: una perspectiva social hacia el desarrollo del aprendizaje en una lengua extranjera)

    ERIC Educational Resources Information Center

    Gómez Lobatón, July Carolina

    2011-01-01

    This pedagogical innovation aims at discovering new ways of interaction that go beyond the unidirectional relationship that is presented in the classrooms most of the times. The innovation considers peers to be active agents in the construction of knowledge and proposes new ways to arrange groups in the classroom so that the arrangement can…

  15. Historia de vidas de profesoras de ciencias: Hacia una ensenanza cientifica de calidad

    NASA Astrophysics Data System (ADS)

    Vega Osorio, Melba

    The purpose of this research was to capture the memories, practices, thoughts and philosophies of education that four experienced Science teachers have accumulated through the years. The life story qualitative research design was used in order to collect more meaningful information from the participants amenable to in depth analysis and interpretation. The life stories interviews were conducted using an interview protocol especially designed for the study. The interviews were recorded and transcribed. The questions that guided this research are: 1. What are the meanings that science teachers give to their educational practices? 2. How did the life experiences of the participants contribute to their success as science teachers? 3. How did the participants' educational practices change and evolve over time? 4. What does teaching science mean in the life of the participants? 5. Which reflections arise from the work of the participants that impact the teaching of science? 6. What are the participants' conceptions about teachers' professional development? 7. What are the participants' contributions to the field of scientific education? Wolcott's (1994) DAI analytical model was used for the analysis of the information and the narratives were written following the three dimensions of the model: description, analysis and interpretation. According to the life stories of the participants, being a teacher requires more than teaching the content. To be a successful and an exemplary science teacher, they have to live the teaching vocation, with dedication, passion, commitment and love for teaching, for students and science; teachers have to give their best for the students, even if it means making personal sacrifices; they should keep updated in knowledge (content) and in educational innovations (strategies); emphasizing both the theoretical and the practical aspects in the field of science, and being an inspirational guide for students.

  16. Inhibición de Paenibacillus larvae empleando una mezcla de aceites esenciales y timol

    Microsoft Academic Search

    S. R. FUSELLI; S. B. GARCÍA DE LA ROSA; L. B. GENDE; M. J. EGUARAS; R. FRITZ

    Inhibition of Paenibacillus larvae employing a mixture of essential oils and thymol. In vitro antimicrobial activity of a mixture of two essential oils and thymol against Paenibacillus larvae, causal agent of American Foulbrood (AFB), was evaluated. The essential oils were extracted from cinnamon (Cinnamomum zeylanicum) and thyme (Thymus vulgaris). The third component used, thymol, is the major component of the

  17. Implementación de una estrategia de aprendizaje cooperativo en la plataforma educativa Moodle

    Microsoft Academic Search

    Rolando Rodríguez González; Nuria Medina Medina

    2011-01-01

    El presente trabajo se enmarca en el uso pedagógico de las TIC principalmente en el uso de estrategias de aprendizaje para el trabajo grupal de los estudiantes con el uso de la plataforma educativa MOODLE. Se implementó un nuevo módulo en dicha plataforma para el aprendizaje cooperativo basado en el método JIGSAW. Además se diseñó un nuevo formato de curso

  18. Borders and Identity: A Resource Guide for Teachers = Identidad y Fronteras: Una Guia para Maestros.

    ERIC Educational Resources Information Center

    Belauus, Betty; Botein, Emily; Cadaval, Olivia

    The materials in this resource guide include a four-part video, a poster-size cultural map with additional exercises, and the five sections of this guide. The unit, presented in English and Spanish, intends to introduce students to the peoples and cultures of the U.S.-Mexico border, to explore the concept of borders in their own communities, to…

  19. LA TRANSVERSALIDAD CURRICULAR EN EL CONTEXTO UNIVERSITARIO: UNA ESTRATEGIA DE ACTUACIÓN DOCENTE

    Microsoft Academic Search

    FERNÁNDEZ BATANERO; NERVA VELASCO REDONDO

    2003-01-01

    Within the framework learning of the university education, the curricular transversalidad constitutes an alternative like strategy of educational per- formance to improve the quality of the formation processes. This strategy must constitute an average one to connect the learning of the curricular con- tents with the learning of the procedures and strategies, to learn more and better the university contents,

  20. Diseno de una plataforma de agentes compatible con FIPA para dispositivos limitados

    Microsoft Academic Search

    Guillermo D ´; Ma Celeste Campo Vazquez

    Recent advances in micro-electronic and wireless technologies have fostered the prolifera- tion of small devices with limited communication and processing power. We think that agent technology will be of great help in pervasive systems development. Pervasive systems are inher- ently dynamic, with devices continually coming and going. Agents are autonomous software entities that can interact with their environment, and therefore

  1. Modelado de Servicios Complejos en una Plataforma de Intermediación para Comercio Electrónico

    Microsoft Academic Search

    Enrique Vázquez; Francisco Valera; Luis Bellido

    This paper describes a brokering platform for the provision of complex services over the Internet. The platform is able to analyse a request for a complex service, divide it into simple components, and combine several services and products, offered by different providers, into a solution that satisfies the complex request. The platform helps the user to refine or change the

  2. Per una didattica con il laboratorio linguistico (Teaching with the Language Laboratory)

    ERIC Educational Resources Information Center

    Freddi, Giovanni

    1977-01-01

    Maintains that the language laboratory is the best way to aid language students in developing linguistic competence. The laboratory equipment needed is described and a pedagogical program for its effective use is outlined. (Text is in Italian.) (CFM)

  3. L'utilit\\`a di una teoria inutile-Crittografia, firma digitale e teoria dei numeri

    E-print Network

    Esposito, Alfredo

    2011-01-01

    The theory of numbers was supposed to be the less useful branch of mathematics. At the same time, cryptography was thought to be a military or a diplomatic issue. In this note we show how the two concepts are today strictly related and how this link affects our daily activities.

  4. UNA PROPUESTA DE EMPALME DE LAS ENCUESTAS DE HOGARES BASADA EN LA TASA DE DESEMPLEO

    Microsoft Academic Search

    JUAN BYRON CORREA; Sociología del Trabajo

    An analysis of the changes in the methodology for conducting the Na- tional Household Survey (NHS) and the Continuous Household Survey (CHS) reveals a divergence in the approaches to reviewing labor mar- ket indicators, thus warranting the development of a proposal for consoli- dating variables used in calculating labor market indicators. This article specifically discusses the consolidation of a series

  5. Una discusión sobre la curva de Phillips de Friedman y la tasa natural de desempleo

    Microsoft Academic Search

    Leonardo Raffo López

    2007-01-01

    In this paper I evaluate the theoretical consistency of Friedman.s Phillips curve. For this, I review his own exposition in the subject, making emphasis in his contributions to the short-run analysis of unemployment, wages and prices in front of the classical theory and Keynes.s .General Theory.. I examine his hypothesis founded in the natural rate of unemployment, looking at the

  6. Per una pedagogia della comprensione interculturale (A Method for Teaching Intercultural Understanding).

    ERIC Educational Resources Information Center

    Titone, Renzo

    1986-01-01

    Defines true bilingualism as biculturalism, explains what "understanding" another culture means, and describes how to evaluate the effectiveness of teaching methods for students from different cultures. Ideally, individuals learning a second language will acquire a "metacultural" consciousness that permits them to be comfortable in any cultural…

  7. Una trama particular: pedagogía ciudadana, instituciones, proceso histórico y liderazgo; Bogotá 1995-1997

    Microsoft Academic Search

    Edgar Yesid; Sierra Soler

    2004-01-01

    The debate about the civic education is an effective topic for the calendar of the go- vernments in the nascent Latin American democracies. Diverse investigations, programs and curriculum have been in charge of this topic. The efforts have been centered in the school environment and various works recommend the use of pedagogy and the installation of certain type of school

  8. Areas Nacionales de Estudio Ambiental: Una Guia. (National Environmental Study Area: A Guide).

    ERIC Educational Resources Information Center

    National Park Service (Dept. of Interior), Washington, DC.

    This booklet, the Spanish version of SE 014 817, is a guide for teachers and resource managers who are interested in establishing National Environmental Study Areas (NESA) or interested in receiving NESA recognition for their on-going environmental study area programs. It outlines the characteristics and procedures of the program; the nature,…

  9. Mapas conceptuales: una experiencia pedagógica en la construcción de un conocimiento significativo en las Ciencias Sociales

    Microsoft Academic Search

    Yamilic Palencia

    The research takes place in the Cardonal Wayuu Basic School located in the city district of Indio Mara, Idelfonso Vásquez parish, municipal area of Maracaibo, Zulia state. It is aimed at the second stage of Basic Education, and the main objective is to propose Conceptual Maps as a pedagogical strategy in the construction of meaningful knowledge and the development of

  10. AE0200 CINEMA AL CAMPUS Descripci: els amants del cinema tenen la possibilitat de veure una

    E-print Network

    Oro, Daniel

    AE0200 CINEMA AL CAMPUS AE0210 AE0220 Descripció: els amants del cinema tenen la possibilitat de Sessions/durada: 30 / 75h ACTIVITAT COMPUTABLE A LES ASSIGNATURES: (consultau-ne les guies docents) AE0210 CINEMACAMPUS AE0211 AE0212 Descripció: la idea de donar a conèixer les particularitats del cinema com

  11. ltima modificaci: 10-06-2013 200001 -CV -Clcul en una Variable

    E-print Network

    Casanellas, Marta

    fent servir per a cada cas l'entorn computacional escaient. 4. CB-1. Demostrar posseir i comprendre. Assimilar la definició d'un nou objecte matemàtic en termes d'altres ja coneguts i ser capaç de fer servir

  12. LOS SISTEMAS INTEGRADOS DE GESTIÓN. UNA ALTERNATIVA METODOLÓGICA PARA SU DISEÑO E IMPLANTACIÓN

    Microsoft Academic Search

    Mayra R. Moreno Pino; María del Carmen Proenza Rivas; Martín Alberto Ayala López; Rossio Coya Cáceres; Iosvani Segura Leyva

    2011-01-01

    En este artículo se presentan consideraciones que facilitan la implantación de los sistemas de gestión en organizaciones que tienen implantado, o pretenden implantar, el sistema de dirección y gestión empresarial conforme a lo establecido en el decreto No.281 del Comité Ejecutivo del Consejo de Ministros de la República de Cuba y como parte de este los sistemas de control interno

  13. Phase unwrapping of MR images using Phi UN--a fast and robust region growing algorithm.

    PubMed

    Witoszynskyj, Stephan; Rauscher, Alexander; Reichenbach, Jürgen R; Barth, Markus

    2009-04-01

    We present a fully automated phase unwrapping algorithm (Phi UN) which is optimized for high-resolution magnetic resonance imaging data. The algorithm is a region growing method and uses separate quality maps for seed finding and unwrapping which are retrieved from the full complex information of the data. We compared our algorithm with an established method in various phantom and in vivo data and found a very good agreement between the results of both techniques. Phi UN, however, was significantly faster at low signal to noise ratio (SNR) and data with a more complex phase topography, making it particularly suitable for applications with low SNR and high spatial resolution. Phi UN is freely available to the scientific community. PMID:19070532

  14. Inventarios Globales de Biodiversidad: Una Respuesta a la Crisis Taxonómica

    NSDL National Science Digital Library

    Lawrence M. Page (University of Florida; )

    2006-05-01

    Taxonomy, the naming and classification of species, is in crisis. Planetary Biodiversity Inventories (PBIs) offer a way to increase the identification of organisms, our knowledge of Earth's biodiversity, student enrollment in taxonomic studies, and dissemination of information about the planet's species.

  15. Esercizi e complementi quattordicesima settimana 1 Determinare una primitiva delle seguenti funzioni.

    E-print Network

    Marconi, Umberto

    ), l'équation de la chaìnette n'est pas modifiée, c'est-à-dire que l'axe OY est un axe de symétrie de'appelle une cycloìde. Prenons pour axe OX la droite sur laquelle roule le cercle ; pour origine des l'axe OX et soit t l'angle dont a tourné le cercle. Soit de plus C le centre du cercle, N le point

  16. Cultura, genes y epidemiología evolutiva en los trastornos del comportamiento alimentario: una polémica interesante

    Microsoft Academic Search

    2005-01-01

    Introduction and objectives: This paper explores Eating Disorders (ED) as a culture-bound syndrome and discusses the environmental and genetic roles in their etiology. The paradox of self-starvation amidst wealth is revised, as well as, socio-cultural pressure of an ideal thin

  17. REFLEXIONES PRELIMINARES SOBRE UNA APLICACIÓN CIENTÍFICO-MÉDICA DE ACTUALIDAD: LA CLONACIÓN

    Microsoft Academic Search

    José Antonio Arranz; Xavier Ariza; Encarnació Riudor; Lluis Vilar; Jordi Salvadó; Núria Galofré; Susana Vendrell; Josep María Esquirol

    2003-01-01

    Resumen: Este artículo afronta el nuevo reto que la tecnociencia médica ha abierto: la posibilidad de clonación terapéutica o reproductiva. En el presente trabajo se aborda, clara y esquemáticamente, la terminología científico-médica, desde los conceptos de reproducción sexual o asexual hasta la endonucleación, pasando por los conceptos de embrión gamético, somático, de cultivo y células madres, para ir realizando un

  18. La Responsabilidad Social Empresarial en Uruguay. Una visión comparada desde la ciudadanía y desde las empresas

    Microsoft Academic Search

    Zuleika Ferre; Natalia Melgar; Héctor Pastori; Giorgina Piani; Máximo Rossi

    2007-01-01

    The aim of this paper is to present, in a comparative view, the results of the Survey: Corporate Social Responsibility (CSR) which was carried out by the Department of Economics (FCS-UDELAR) in 2006 to respondents older than 17 years and to private companies in the industry, commerce and services during the second quarter of 2007. The main purposes of both

  19. Una respuesta a la acción social. De la rentabilidad a la responsabilidad social empresarial

    Microsoft Academic Search

    Ana María Vélez Evans

    2010-01-01

    Durante las décadas de los 70 y 80 el mundo observó el surgimiento y consolidación de la Escuela de Chicago, liderada por Milton Friedman, quien argumentó que la única responsabilidad social de las empresas era obtener los mayores beneficios respetando el marco legal vigente y dando cumplimiento de los criterios éticos para no incurrir en engaño. Esta tesis tenía de

  20. Renovando la Esperanza por una Educacion sin Exclusiones (Rekindling the Hope for an Education without Exclusion).

    ERIC Educational Resources Information Center

    Revista Interamericana de Educacion de Adultos, 2001

    2001-01-01

    Articles in this issue, written in Spanish, focus on the following: current status and outlook of youth and adult education; opening statement of the 50th anniversary commemoration; regional framework for the education of youth and adults in Latin America and the Caribbean; interculturalism and the education of youth and adults; participation of…

  1. TENDENCIAS DE PUBLICACIÓN EN SALUD PÚBLICA EN COLOMBIA: UNA COMPARACIÓN INTERNACIONAL

    Microsoft Academic Search

    Andrés Ignacio Vecino; Catalina Latorre Santos; Hernán Jaramillo Salazar

    2008-01-01

    El siguiente documento muestra las diferencias temáticas y metodológicas de la publicación internacional en salud pública con respecto a la producción científica de las revistas colombianas. Se seleccionaron 5 revistas, de alto impacto y con indización internacional de carácter general de salud pública en el período de 1991-2005, y se compararon por la complejidad de la metodologías utilizadas para el

  2. Una Experiencia Pedagogica en el Jardin Infantil "La Cabana" (A Teaching Experience in "La Cabana" Kindergarten).

    ERIC Educational Resources Information Center

    de la Vega, B.

    1992-01-01

    Reviews the history of La Cabana kindergarten, which provides preschool education and meals for 100 children and sewing classes for mothers in a working class neighborhood in Bogota, Colombia. Describes the implementation of a new curriculum which develops child creativity through free choice of activities around a common project. (AC)

  3. Pronóstico tras una agudización grave de la EPOC tratada con ventilación mecánica no invasiva

    Microsoft Academic Search

    Jose Echave-Sustaeta; Lorena Comeche Casanova; Ricardo García Luján; Javier Sayas Catalan; Agustin Gómez de la Cámara; Angel López Encuentra

    2010-01-01

    IntroductionPatients with chronic obstructive pulmonary disease (COPD) who survived an acute exacerbation with acute respiratory failure that required non-invasive mechanical ventilation (NIMV) are a group with a poor medium-term prognosis.

  4. El suicidio: más que una noticia, un tema de responsabilidad social

    Microsoft Academic Search

    Henry Rubiano Daza; Tatiana Quintero Cabrera; Clara Inés; Bonilla Londoño

    2007-01-01

    Suicide is undoubtedly a fact of great interest to mass media because of its social impact. However, there is great concern among the academic community and the critical mass about how this type of news are presented, the language used and the stylistic manage- ment of the fact, especially if we realize that teenagers and young people are vulnerable to

  5. Desarrollo de una intervención grupal para la prevención del VIH en parejas heterosexuales1

    PubMed Central

    Pérez-Jiménez, David; Escabí Montalvo, Aracelis; del Carmen Cabrera Aponte, María

    2012-01-01

    HIV preventive efforts with heterosexual couples are almost nonexistent. We designed a group intervention based on the Information-Motivation-Behavioral Skills model with the aim of increasing male condom use and promoting the practice of mutual masturbation as a safer sex alternative. We carried out a pilot study with four couples to evaluate the feasibility of the intervention and the aspects of content and format that needed to be modified. Findings reflect that participants were very satisfied with the intervention and they found it entertaining and innovative. They were very comfortable with its format, as well as with the activities. The findings confirm the feasibility of this intervention and the need to direct preventive efforts to this population. PMID:23847716

  6. UNA VISIÓN CRÍTICA DE LA NARRACIÓN DE ARTHUR GORDON PYM DE EDGAR ALLAN POE

    Microsoft Academic Search

    DANIEL FERREIRA PAREDES; CÉSAR WEE QUIJADA

    As Poe's fans and followers of his writings results of critical responsability to relocate The Narrative of Arthur Gordon Pym through the advances of literary studies of the XXI century. In order to achieve it, this research uses reviews, criticism and views of two centuries, which flow to grant us a \\

  7. El gerenciar la base de datos con el análisis multivariado: una visión diferente del cliente

    Microsoft Academic Search

    Harold Lee Koch; Rajshekhar G. Javalgi

    1998-01-01

    La segmentación del mercado y el conocer al cliente es aún más importante hoy en día para contrarrestar los efectos de la recesión y la poderosa competencia que se ha presentado por la apertura del mercado latinoamericano y simultáneamente aprovechar las tendencias hacia la fusión, la adquisición y la consolidación del mercado. Este artículo pretende promover Investigaciones de Mercado más

  8. FUNCIÓN SOCIAL DE LAS LÁGRIMAS: UNA INDAGACIÓN EMPÍRICA SOBRE LOS TIPOS DE LLANTO EMOCIONAL

    Microsoft Academic Search

    OSCAR SIERRA FITZGERALD; BEATRIZ E. MEJÍA CONSTAÍN

    Human emotional interchange implicates expression\\/recognition of emotions. The human face is a conspicuous place to express\\/read emotion. Certain emotions associate with emotional tearing, differentiable from basal and reflex tearing. Murube, Murube and Murube (1999) classified emotional tearing in requesting- and offering -help. The validity of that typology was evaluated using faces of people of both sexes crying because of their

  9. El efecto invernadero producido por el CO2 atmosférico: una nueva interpretación termodinámica

    Microsoft Academic Search

    EDUARDO R ABRIL

    The glasshouse effect caused by atmospheric CO2: a new thermodynamic interpretation: At present times the use of models to simulate and predict the climate change and its consequences on global ecological processes has contributed to a better understanding of the phenomena in several disciplines. However, there is evidence that current models fail to simulate a number of well known climatic

  10. La paradoja de los 80's: Una visión particular del teatro en México

    E-print Network

    de Ita, Fernando

    1992-04-01

    conjunto a partir de algunos elementos fundamentales para la comprensión de la intrincada paradoja que mata y da vida al teatro en México. Para cualquier análisis de la producción artística del país hay que tomar en consideración la crisis económica y... la enseñanza.1 Si alguna crisis tuvo el teatro mexicano de los ochenta esta fue la del modo de producción que afectó al país en todos los órdenes. Para 1982 la Nación que según el presidente José López Portillo debería prepararse para vivir en la...

  11. Una Aplicación de la Teoría de Juegos Cooperativos a la Descomposición de la Pobreza en México

    Microsoft Academic Search

    Juan Carlos Chávez Martín del Campo; Horacio González Sánchez; Hector Villarreal Páez

    2010-01-01

    El presente artículo estima la descomposición de la variación en la pobreza en México aplicando algunos resultados derivados de la teoría de juegos cooperativos. En particular, se utiliza el enfoque del valor Shapley (Shapley 1953) para estimar la contribución del crecimiento, la distribución del ingreso y los precios en la descomposición. La disminución observada en la familia P_alpha de medidas

  12. Una nova estimacio retrospectiva del VAB regional industrial. Espanya (1860-1930)

    Microsoft Academic Search

    Daniel A. Tirado; Julio Martinez-Galarraga

    2008-01-01

    This article offers a new historical dataset of industrial GVA for Spanish provinces (NUTSIII) and autonomous regions (NUTS II). For doing that, a new methodology is proposed in order to estimate historical figures of regional GVA. Traditional estimates of regional industrial output in Spain have usually been based on fiscal sources. These data allowed the estimation of regional industrial capital

  13. Hacia una Metodología para la Construcción de Modelos de Simulación Basados en Sistemas Multi-Agente

    Microsoft Academic Search

    Julián Moreno Cadavid; Juan D. Velásquez; Demetrio Arturo Ovalle Carranza

    2005-01-01

    Palabras Clave: Simulación basada en Sistemas Multi-Agente, Metodologías de Desarrollo de Modelos. Abstract: The aim of this paper is to show the different phases, models and artifacts of a methodology that was created by the research group in order to develop multi-agent based simulation models. The proposed methodology is based on some of the most outstanding features of the classic

  14. La guerra del fin del mundo: historia e invencion en una novela historica

    E-print Network

    Loeffler, Lisa Bowe

    1984-01-01

    41 67 78 IV CONCLUSION 98 APEND ICE NOTAS BIBLIOGRAFXA 104 131 135 VITA 138 CAPITULO I INTRODUCCION La novels mas reciente del escritor peruano contemporaneo Marin 1 Verges Llosa, La uerra del fin del mundo, trata un movimiento reli... novelists y corno critico (Oviedo, Invencion, 310). SegGn el, este terna le fascina porque lo 4 entiende menos cuanto mas lo practica. Puesto que los comentarios de Verges Llosa en sus obras de critics con respecto a otros novelistas se aplican...

  15. IMPLEMENTACIÓN DE UN CANCELADOR DE CROSSTALK EN UNA PLATAFORMA DE BAJO COSTE

    Microsoft Academic Search

    Óscar Esteban Moreno; Alberto González; José Javier López

    The crosstalk problem in the loudspeaker-ear system is well known by the audio processing community. If the adequate filter bank is not used, the sound from each loudspeaker will be heard by the improper ear with a delay and a response dependent on the head. This effect is undesirable in applications like binaural reproduction. We propose to implement a crosstalk

  16. Filosofía de la Tecnología y Riesgo Tecnológico. Una confrontación con los riesgos y las tecnologías

    Microsoft Academic Search

    Iván Mateos Riaño

    The technological systems are manifested in the gnoseolgical analisis like a group of knowledge and social structurally deficient's social practice the theorical key for the tematizacion of such deficits is that of \\

  17. C R E D I T S Co-editors: Una Moneypenny and Nora Balfour

    E-print Network

    Keinan, Alon

    , Networks, and Distributed Computing 13 Scientific and Parallel Computing 13 Security 13 Theory of Computing Computing and at Cornell CIS C O N T A C T I N F O R M A T I O N Office of Computing and Information Science.cs.cornell.edu #12;2003 Education C O N T E N T S Annual Report and Research // Information Science Message from

  18. REVISION HISTORICA DELCONCEPTO DE INTELIGENCIA: UNA APROXIMACION A LA INTELIGENCIA EMOCIONAL

    Microsoft Academic Search

    CARMEN MOLERO MORENO

    A historical review of the eonceptualization of intelligence is presented, from the beginning of scientific psychology up lo the end ofthe XXth. century. Ernphasis is placed on the most recent periodo The psychometric, biological and innatist perspectives are seen in a different Iight in contemporary research. The present conceptualization of emotional intelligence ineludes not only the cognitive world but also

  19. Contexto El proyecto Temas de paralelismo Perspectivas Una experiencia de iniciacion al paralelismo en segundo curso

    E-print Network

    Giménez, Domingo

    ´in Cervera, Domingo Gim´enez Departamento de Inform´atica y Sistemas M. Carmen Garrido, Juan A. S´anchez Laguna Departamento de Ingenier´ia de la Informaci´on y las Comunicaciones Jos´e Guill´en, Juan Alejandro paralelismo Perspectivas La computaci´on paralela en la actualidad Los sistemas computacionales son paralelos

  20. El analisis sintactico superficial o la simplificacion de una tarea compleja

    E-print Network

    Pla, Ferran

    de palabras Sistemas Di´alogo ¡ An´alisis del lenguaje coloquial Extracci´on de Informaci´on´on `cl´asica' Representaci´on de la informaci´on sint´actica. ¡ redes de transici´on, gram´aticas, etc´alisis ascendente M´as informaci´on en: [Allen, 1995],[Moreno et al., 1999], ... SEPLN-2002. Valladolid, 11

  1. Interacción entre Susceptibilidad Hipnótica y Experiencias Disociativas en una Población que Reporta Experiencias Anomalo\\/Paranormales

    Microsoft Academic Search

    Alejandro Parra; Juan Carlos Argibay

    2006-01-01

    There are reliable statistical relationships between hypnotic susceptibility, psychological dissociation, and paranormal experiences. We hypothesized that subjects with greatest hypnotic susceptibility would report the most paranormal experiences. Similarly, we expected that persons with the most dissociative experiences would report the most paranormal experiences. We also hoped to find interactions between hypnotic susceptibility and dissociation with paranormal experiences. We administered the

  2. Vers una teoria global de defensa química en plantes: el cas de alcaloides

    Microsoft Academic Search

    Eva Castells; Josep Peñuelas i Reixach

    1997-01-01

    Towards a global theory of chemical defense: The case of alkaloids Alkaloids are a large and very diverse group of secondary metabolites found principally in angiosperms. They play a defensive role in plants against herbivores and pathogens. Alkaloid-bearing plants are usually short life plants and they are localized preferently in tropical regions. Alkaloid concentrations vary in different plant parts and

  3. Anemia y deficiencia de hierro en niños menores de cuatro años de una localidad en Valencia

    Microsoft Academic Search

    Liseti Solano; María Adela Barón; Armando Sánchez Jaeger; María Páez

    Abstract Abstract Abstract Abstract. Iron deficiency with or without anaemia is one of the most frequent nutritional deficiencies in Venezuela, especially in socioeconomic deprived population. Due to the higher risk for children below four years of age for this deficiency, assessment of prevalences was determined by a descriptive and cross sectional study on 543 chil- dren from 6 to 48

  4. Sobre Como Varian las Firmas Manuscritas con el Tiempo: una Modelizacion Sigma Lognormal

    E-print Network

    Autonoma de Madrid, Universidad

    de edad basado en los par´ametros del modelo. 1 Introducci´on Como en cualquier nuevo ´area de- conocimiento, con el objetivo de conseguir menores tasas de error. Como conse- cuencia, se han pasado por alto nueva aproximaci´on para el estudio de firmas pertenecientes a grupos de edades dis- tintas. #12;La

  5. Relacion de problemas 6: Estadistica descriptiva en una variable Matematicas II, Grado en Optica y Optometria

    E-print Network

    Hurtado, Ana

    ´aximo del 10 % de los ni~nos que tienen menor peso? 4. En un aparcamiento cobran por cada minuto que est~no 2000 por edad entre un millar de nuevos enfermos es la siguiente: Edad [0,10] (10,20] (20,30] (30, 40] (40,50] (50,60] (60,70] no Enfermos 23 12 420 395 103 34 13 Determina: (i) La edad media de contagio

  6. Gulliver dormido de Samuel Rovinski: Una parodia del discurso del poder

    E-print Network

    Rojas, Mario A.

    1990-10-01

    espaciales y actuando de acuerdo a las condiciones de un acto de habla plenamente acabado (o incumplido por trasgresiones a los postulados básicos de la comunicación). Además siguen reglas de conducta similares y los mismos actos ilocutivos del habla...), en el deber ineludible de explorar y señalar la verdad de los acontecimientos sociales, en la misión de un teatro que eduque mientras entretiene y que sabe despertar el espíritu crítico del público, me siento comprometido por mis FALL 1990...

  7. Desafíos para una agricultura con bajas emisiones de carbono y conservación forestal en Brasil

    Microsoft Academic Search

    Britaldo Soares Filho; Letícia Lima; Maria Bowman; Letícia Viana

    2012-01-01

    Este artículo se examina la retroalimentación que existe entre cambio climático, deforestación y expansión agropecuaria, y presenta escenarios relativos a la demanda agropecuaria y a las políticas de conservación y recuperación forestal en Brasil. Asimismo, se examinan las implicancias de estos escenarios para el abastecimiento de alimentos y carne, la provisión de servicios de los ecosistemas, y la mitigación del

  8. Hacia una nueva organización de los dispositivos de salud pública en España. Informe SESPAS 2008

    Microsoft Academic Search

    Carlos Artundo Purroy; Ana Rivadeneyra Sicilia

    2008-01-01

    Public health in Spain shows significant weaknesses. Spanish public health services respond reasonably well in crisis situations but tend to be invisible and occupy a marginal position in political agendas and in relation to health services. The organization of the public health subsystem is clearly out of date in terms of its ability to promote and protect community health, to

  9. Una Aproximación basada en i* para el análisis de Requisitos de Seguridad en Almacenes de Datos

    Microsoft Academic Search

    E. Soler; V. Stefanov; J. N. Mazón; J. Trujillo; E. Fernández-Medina; M. Piattini

    Resumen— Las propuestas de análisis de requisitos para almacenes de datos (AD) se centran únicamente en las necesidades de información de los usuarios, sin tener en cuenta otro tipo de requisitos como la seguridad o el rendimiento. Sin embargo, el modelado de estos aspectos en etapas tempranas del desarrollo es fundamental para obtener un AD que satisfaga las expectativas del

  10. The digital language teacher Associate Professor Una Cunningham grew up in Northern

    E-print Network

    Hickman, Mark

    pronunciation with English, Swedish and Spanish as target languages. Her recent work has been developing e-learning long been an area where technology has been brought to learners and there is considerable potential for intelligently using widely available technology to further teachers' and learners' proficiency in the target

  11. Una propuesta destinada a complementar el cuestionario Font-Roja de satisfacción laboral

    Microsoft Academic Search

    Eduardo Núñez González; Gabriel J. Estévez Guerra; Pablo Hernández Marrero; Carmen Delia Marrero Medina

    2007-01-01

    IntroductionSeveral studies have shown the need to include the physical work environment among the dimensions included in job satisfaction evaluation. However, this dimension was not included in the Font-Roja questionnaire. The present study introduces two items exploring this dimension and adheres to the hypothesis that physical work environment has a significant impact on job satisfaction evaluation.

  12. Impacto de una intervención de autoevaluación clínica sobre la adecuación de la estancia hospitalaria

    Microsoft Academic Search

    Jaume Monteis Catot; Montserrat Martín-Baranera; Nikita Soler; Josep Vilaró; Carlos Moya; Francesc Martínez; Marta Riu; Carme Puig; Antoni Riba; Gemma Navarro; Assumpta Espinagosa; Genís Carrasco Gómez; Xavier Castells; Salvador Peiró

    2007-01-01

    AimTo assess the impact of an intervention on inappropriate hospital stays (IHS) in acute-care hospitals in Catalonia (Spain) with the aim of testing the hypothesis that a simple intervention (adeQhos®) reduces the proportion of IHS.

  13. La información en tiempo real. Una herramienta necesaria en vigilancia epidemiologica

    Microsoft Academic Search

    Francisco González Morán; Isabel Muñoz Criado; Hermelinda Vanaclocha

    2008-01-01

    Since 2004 an electronic epidemiological surveillance system (AVE) has been developed and implemented for infectious diseases in the autonomous region of Valencia that allows data collection from notifiable diseases in real time concerning outbreaks and alerts, as well as analysis and dissemination of the information. Within the autonomous region of Valencia, the system is used by 17 epidemiology units, which

  14. PREVENCIÓN DEL VIH/SIDA EN LOS CIRCUITOS DE LEVANTE HSH: UNA ASIGNATURA PENDIENTE1

    PubMed Central

    Barreda, Victoria; Carballo-Dieguez, Alex; Marone, Rubén; Balán, Iván; Pando, María Ángeles; Ávila, María Mercedes

    2011-01-01

    Resumen A partir de un relevamiento de tipo etnográfico, se describen lugares de encuentro de HSH en la Ciudad de Buenos Aires y sus prácticas sexuales. El reconocimiento de tales espacios, así como las características que asumen en ellos los encuentros sexuales entre los HSH, plantean obstáculos específicos en la adopción de comportamientos preventivos y, asimismo, generan nuevos desafíos para las actividades de prevención. Se plantean las dificultades y debates conceptuales que la misma categoría presenta, y sus consecuencias en el abordaje preventivo y teórico-metodológico para las ciencias sociales. Además, se proponen nuevos interrogantes acerca de los alcances y las limitaciones del modelo preventivo del VIH/Sida para HSH. PMID:21874154

  15. FAMILIAS DESMEMBRADAS Y ORFANDADES: REPRESENTACIONES DE UNA ACTITUD POSMODERNA HACIA LA NACIÓN MEXICANA

    E-print Network

    Tolentino, Adriana

    2009-12-10

    The image of the traditional patriarchal family as the perfect allegory for the nation has been widely used in Latin American literature since the 19th century. Within the frame of postmodernism, this study challenges and ...

  16. Evaluación paramétrica y no paramétrica del desempleo pesquero en Marruecos: Una aplicación para el caso andaluz

    Microsoft Academic Search

    Encarnación Cordón Lagares; Félix García Ordaz

    2010-01-01

    RESUMEN El principal objetivo es el análisis de las medidas que las diferentes administraciones pusieron en marcha para reducir las altas tasas de desempleo en la población andaluza afectada por la paralización de la actividad pesquera como consecuencia de la finalización del Acuerdo de Pesca entre la Unión Europea y Marruecos desde el 30 de noviembre de 1999. Mediante la

  17. Pholcus vachoni sp. n. (Araneae, Pholcidae) una nueva especie de Agadir (Marruecos)

    Microsoft Academic Search

    Dimitar Dimitrov; Carles Ribera

    A new species of the genus Pholcus (Araneae, Pholcidae) from Agadir (Maroc co) is described. Some comments about possible relationships with Pholcus species from the canarian archipelago, which presents important morphological similarities, are included. This is the first species of this genus described from

  18. Ascaris lumbricoides: Una revisión de su epidemiología y su relación con otras infecciones

    Microsoft Academic Search

    Marilyn E. Scott

    2008-01-01

    ResumenEn esta revisión se realzan los progresos efectuados desde 2004 en el conocimiento de la epidemiología de la infección y las interacciones entre Ascaris lumbricoides y otras infecciones concomitantes. A medida que aumenta la escasez de agua se utilizan cada vez más aguas residuales no tratadas para regar cultivos, aumentando de este modo el riesgo de transmisión. Se describen métodos

  19. Habitos Alimentarios e Higiene Dental en una Poblacion Preescolar de Madrid.

    ERIC Educational Resources Information Center

    Lafarga, R. Tarazona; And Others

    1991-01-01

    Nutriment habits and oral hygiene in a preschool population in Madrid were studied by stratification in public or private schools. Significant differences were found only with regard to consumption of fish. Vegetable and fruit consumption was low in both groups, but not statistically significant. (SH)

  20. Al fin una herramienta que ahorra tiempo al investigador de la Universidad de Chile!

    E-print Network

    Matamala, Martín

    Oxford University Press Ovid Journals Professional Development Collection ProQuest Computing ProQuest Psychology Journals Proquest Research Library ProQuest Science Journals Psychology & Behavioral Sciences

  1. Una nueva galaxia IR con nucleo compuesto: Seyfert 1.5 y estallido de formacion estelar

    NASA Astrophysics Data System (ADS)

    Lipari, S. L.; Merlo, D. C.; Moyano, M. M.

    Optical aperture spectroscopy and CCD broad- and narrow-band images are presented for the barred Seyfert 1 galaxy: IRAS 22117-3903. IRAS data are also used to analyse this object. The CTIO broad band CCD imaging show interesting morphological features: a compact nucleus with a prominent bar; two bright spiral arms; and a partial/segmented inner ring. The narrow-band imaging show that the nuclear H+[NII] emission is concentrate mainly in the nucleus. We find optical spectral features characteristic of a Seyfert 1.5 nucleus; i.e.; showing hydrogen emission lines with broad and narrow components. We detected high excitation emission lines ([CaV]+[FeXIV]; [FeVII]). We study the stellar population in the nuclear region. We find that the stellar population that represent quite well the nuclear stellar population is an average of spiral galaxies dominated by the old population plus a contribution of young stars. Physical conditions are derived for the narrow emission line region; and we detected very high electron temperature (T=4400045000 K); untypical for the ordinary AGNs and probably associated to shocks in a starburst component. Finally; at the optical and IR wavelength; we find properties typical of an nucleus with compound nature; in good agreement with the AGN/QSOs evolutionary model of Lípari ; Terlevich (2006; ; 368; 1001). FULL TEXT IN SPANISH

  2. Adaptación de unos dedos biónicos en una paciente con doble amputación de extremidades superiores

    Microsoft Academic Search

    R. Rotllant Solà; C. Navarro Correal; G. Sirvent Batalla; M. Carbajal de la Red; F. Nacenta Arias; M. Gómez Cuba

    2010-01-01

    A case report of bilateral upper limb amputees describing improvement in basic activities of daily living is presented. A 42-year-old female pianist suffered distal necrosis of all limbs and partial amputation of them due to pneumococcal sepsis in 2003. Her left upper limb was prosthetized with a myoelectric prosthesis (infracondilean amputation). In the transmetacarpal amputation of the right upper limb,

  3. Adaptación de una aplicación de e-Learning a t- Learning

    Microsoft Academic Search

    Jonathan Perrinet; Xabiel G. Pañeda; Claudia Acevedo; José Luis Arciniegas; Sergio Cabrero; David Melendi; Facultad de Ingeniería Electrónica; TRABAJOS RELACIONADOS

    2009-01-01

    With the democratization of digital television, the number of applications accessible from the TV is increasing. However, in the case of a transition from a computer application to this new context, new constraints need to be taken into account because of the particular characteristics of this environment. In this paper we revise the usability differences between computers and television, we

  4. Datos epidemiológicos del trastorno por déficit de atención con hiperactividad en una muestra de niños marabinos

    Microsoft Academic Search

    J. A. Peña

    Summary. Aims. This study reports the findings of a research project aimed at determining the rate of prevalence of attention deficit hyperactivity disorder (ADHD) in school-aged children. Patients and methods. Different behavioural and cognitive measures were administered to a sample of children between 3 and 13 years of age obtained by means of a multistage sampling procedure that was stratified

  5. Análisis de causas raíz. Una herramienta útil para la prevención de errores

    Microsoft Academic Search

    Pedro Ruiz-López; Carmen González Rodríguez-Salinas; Juan Alcalde-Escribano

    2005-01-01

    Tools for causal analysis began to be used in industry in the 1970s in order to develop safer systems. These methods had a particular impact on the nuclear and aviation industries, among others. In the health sector, based on techniques already used in other fields, several associations have instigated well-structured methodologies for root cause analysis, inspired by the concepts developed

  6. Incidencia de bacteriemia asociada a catéter venoso central en una unidad de cuidados intensivos

    Microsoft Academic Search

    M. Espiau; M. Pujol; M. Campins-Martí; A. M. Planes; Y. Peña; J. Balcells; J. Roqueta

    2011-01-01

    BackgroundCentral line-associated bloodstream infection (CLABSI) is one of the most common nosocomial infections. The incidence is higher in paediatric patients than in adults, especially in those admitted to Intensive Care Units (ICU). CLABSI-related morbidity makes it a major health problem; therefore it is necessary to develop prevention strategies against it.

  7. Secuenciación de tareas en el ámbito de la producción: una aplicación del algoritmo del reconocido simulado

    Microsoft Academic Search

    Zuleyca Díaz Martínez; José Fernández Menéndez; Paloma Martínez Almodovar

    2004-01-01

    Un problema esencial en la Dirección de Operaciones en entornos industriales y manufactureros es la determinación de la secuencia óptima en la que ejecutar los distintos lotes de productos de manera que se minimicen los tiempos de preparación de máquinas. Se trata de un problema de optimización combinatoria que obliga a utilizar técnicas heurísticas ante la imposibilidad práctica de llevar

  8. Minimización del tiempo total de flujo de tareas en una sola máquina: Estado del arte

    Microsoft Academic Search

    Jairo R. Montoya Torres; Carlos D. Paternina-Arboleda; Yannick Frein

    2002-01-01

    Scheduling on a single-machine is a classic problem on operations research. Lots of methods have been proposed for solving different instances of the problem, depending on the constraints and the type of objective function. In this paper, we are interested in illustrating the state of the art for minimizing total flow time for jobs arriving over time, for both cases,

  9. DE VIAJES Y MIEDOS: LA AGORAFOBIA COMO UNA FORMA DE CONTROL ECONÓMICO

    Microsoft Academic Search

    Maximiliano Korstanje

    2008-01-01

    El objetivo del siguiente trabajo es describir casos análogos de fobia o miedo a los viajes en otras culturas y tiempos diferente a los modernos. En este sentido, consideramos que tanto la cultura aymará como la vida en la antigua roma nos proveen de ejemplos suficientes para considerar a la fobia a los viajes como un problema en extensión. Por

  10. Economía política neoclásica y la América Latina. Una mirada a la bibliografía

    Microsoft Academic Search

    Claudio A Bonilla; Leonardo A. Gatica

    2005-01-01

    Since the beginning of the economic science, politics and economics have been closely related. The study of the interaction between these two fields has originated what we know today as political economy. However, political economy is a broad field that includes a wide range of school of thought that it would be impossible to discuss in one article. The present

  11. Bolivia y una preocupación constante: El indianismo, sus orígenes y limitaciones en el siglo XXI

    Microsoft Academic Search

    Franco Gamboa Rocabado

    Abstract The main objective of this essay is to track all the arguments that con- struct the doctrine called Indian thinking (indianismo). Therefore, the analysis will also be concentrated on the political implications the Indian ideology had inside the Constituent Assembly, where this doctrine denied any pos- sibility to reach a national reconciliation in Bolivia, hindering at the same time

  12. Escalamiento en la Densidad de Im agenes para una Reconstrucci on Tridimensional

    E-print Network

    Fraga, Luis Gerardo de la

    ;ectrica Av. Instituto Polit#19;ecnico Nacional 2508. 07300 M#19;exico, D.F. E-mail: fraga) Todas las proyecciones comparten un mismo origen de coordenadas, es decir, no existen desplazamientos en origen de coordenadas, correctos para todas las proyecciones y nos enfocaremos al punto (3) de los pesos

  13. Migracions i polítiques públiques a Catalunya: una aproximació al paper dels actors

    Microsoft Academic Search

    Xavier Aragall

    2007-01-01

    La presència de persones procedents d'altres països i cultures que entren a formar part de la nostra societat esdevé un dels trets característics de l'actual realitat catalana. Igual com ha succeït en altres societats de l'Europa mediterrània, aquesta nova situació de manifesta pluriculturalitat planteja nous reptes i noves demandes socials i institucionals. Un dels més interessants és, precisament, la incorporació

  14. Reconstrucción de datos de series de tiempo: una aplicación a la demanda horaria de la electricidad

    Microsoft Academic Search

    Elkin Castaño

    Abstract Usually, in the identification and estimation of ARIMA models it is sup- posed that the series to analyze contain neither missing data, nor atypical observations, and interventions do not exist under study period. Neverthe- less, in the practice, these problems can happen simultaneously, affecting the identification of the suitable model,and,therefore his forecasting,capa- city. This article presents,a procedure,that allows to

  15. Puesta en marcha de una unidad de gestión de riesgos clínicos hospitalaria

    Microsoft Academic Search

    Ricardo Pardo; Alberto Jar; Bruno Menchen; David Padilla; Jesús Martín; José Hernández; Jesús Fernández; Dolores Villegas; Carmen López; Purificación Caro; Begoña Llamazares; Javier Fernández

    2009-01-01

    Clinical risk management improves quality in health care by increa- sing patient safety and by trying to eliminate avoidable errors. Im- plementing risk management programs requires the creation of risk management units in hospitals that will be responsible of all the ac- tivities related to patient safety. This article describes the develop- ment one of the first clinical risk management

  16. Puesta en marcha de una unidad de gestión de riesgos clínicos hospitalaria

    Microsoft Academic Search

    Ricardo Pardo; Alberto Jara; Bruno Menchen; David Padilla; Jesús Martín; José Hernández; Jesús Fernández; Dolores Villegas; Carmen López; Purificación Caro; Begoña Llamazares; Javier Fernández

    2005-01-01

    Clinical risk management improves quality in health care by increasing patient safety and by trying to eliminate avoidable errors. Implementing risk management programs requires the creation of risk management units in hospitals that will be responsible of all the activities related to patient safety. This article describes the development one of the first clinical risk management units in Europe directed

  17. GERBERTVS una nuova rivista per l'astronomia e la scienza nell'alto medioevo

    NASA Astrophysics Data System (ADS)

    Sigismondi, C.

    GERBERTVS appears as an international e-journal on medieval science, namely astronomy. It has been created to gather all the studia promoted on Gerbert of Aurillac, the most prominent figure of the tenth century: astronomer, organ builder and music theoretician, mathematician, philosopher, and finally pope with the name of Silvester II (999-1003). Here is presented the first volume (2010) published in the institutional web site www.icra.it/gerbertus with the ISSN 2038-355X.

  18. Procesamiento conjunto de lenguaje e imágenes en contextos didácticos: Una aproximación cognitiva

    Microsoft Academic Search

    José César Perales López; Juan Francisco Romero Barriga

    Title: Language-image integrative processing in instructional contexts: A cognitivist perspective. Abstract: Didactical images are powerful tools to complement written and oral language comprehension in instructional situations. The present work summarizes the most relevant studies on conjoint processing of language and visual displays, focusing on those situations in which the image com- plements a narration (multimedia presentations, oral communications…) or a

  19. 1 rea Temtica (no escribir nada aqu) Utilizacin de una herramienta de comunicacin online para la

    E-print Network

    Grimaldo, Francisco

    la mejora docente. Dos casos prácticos. Francisco Grimaldo-Moreno, Miguel Arevalillo-Hérraez, Emilia oficial universitario, para permitir la participación de profesores desde el extranjero. En ambos casos cooperación institucional, esquemas de movilidad y programas integrados de estudios, de formación y de

  20. AFINALES DE 1974, despus de una desas-trosa combinacin de enfermedades como el

    E-print Network

    Nacional Autónoma de México, Universidad

    alimento y refu- gio del hurón. En 1988, el descubrimiento de las colonias más extensas de perros llaneros(Cynomys ludovicianus) en Norteamérica en la región de Janos- Casas Grandes,nos llevó a proponer este sitio como un área

  1. Homocistinuria: ¿una gran desconocida? Claves para el diagnóstico en atención primaria

    Microsoft Academic Search

    M. C. García Jiménez; A. Baldellou Vázquez

    2009-01-01

    Title: Homocystinurias, a great stranger? Keys for the primary care diagnosis The homocystinurias comprise a congenital error of the sul- phur-containing amino acids metabolism, that with a great etiological and clinical heterogeneity, they possess a series of common features that justify the whole revision of their diag- nosis and treatment. All of them are governed by an autonom- ic inheritance

  2. Perspectiva sobre una Personalidad Senera: Carmen Fischer Ramirez (Perspective on a Singular Personality: Carmen Fischer Ramirez).

    ERIC Educational Resources Information Center

    Quezeda, Dina Alarcon

    1992-01-01

    Traces the career of Carmen Fischer Ramirez, focusing on her work in improving early childhood education in Chile. Reviews her university career, work with the World Organization for Early Childhood Education, and major publications. (AC)

  3. PROPUESTA DE UNA REPARACIÓN SOBRE PLACAS DE FIBRA DE CARBONO SOMETIDAS A IMPACTO BALISTICO

    Microsoft Academic Search

    A. Monsalve; B. Díaz; L. Venegas

    Carbon fibre is a material with increasing use for airplanes construction due to its low specific weight and relatively high mechanical strength. Its use is of particular interest in the construction of combat helicopters, which can be subjected to fire coming from low calibre weapons, typically used by infantry troops. The effect on the mechanical behaviour of carbon fibre sheets

  4. FUNDAMENTOS DE UNA POSIBLE ACCIÓN BENEFICIOSA DE LA Spirulina platensis EN LAS NEUROPATÍAS PERIFÉRICAS

    Microsoft Academic Search

    Nora Sánchez; Margarita Bu; Héctor Pérez-Saad

    Summary Spirulina platensis is a green-blue microalga known by its high nutritional value and considered as one of the most complete natural sources of proteins, vitamins, minerals and other nutrients. The present paper analyzes the potential of this alga in the prevention and treatment of neuropathies on the basis of its main constituents and of the different causes of these

  5. Cefalea recurrente pospunción dural en una paciente con síndrome de Chiari tipo I no conocido

    Microsoft Academic Search

    M. A. Rodríguez-Navarro; J. A. Pérez-Moreno; P. González-Pérez; E. Rubio; E. Manzano

    2009-01-01

    ObjectiveTo identify the risk of neurological complications of spinal anesthesia in patients with preexisting Chiari I malformation and to differentiate this entity from “acquired Chiari I malformation”, caused by intracranial hypotension. These two entities can be difficult to distinguish radiologically.

  6. XII ENCUENTRO DE GEGRAFOS DE AMRICA LATINA "Caminando en una Amrica Latina en transformacin"

    E-print Network

    Boyer, Edmond

    componentes sociales, más allá de la lógica del conflicto. Nuestro objetivo es describir las modalidades del (cuidad de porte medio en el área de inmigración brasileña) en 132 viviendas de inmigrantes brasileños y

  7. ELEMENTOS PARA ORIENTAR UNA POLÍTICA ESTATAL SOBRE ALQUILER DE VIVIENDA URBANA EN COLOMBIA

    Microsoft Academic Search

    Samuel Jaramillo; Marcela Ibáñez

    2002-01-01

    El objetivo de este documento es el de contribuir a diseñar cursos de acción gubernamental en dos ejes: el de promover y activar el mercado de vivienda en alquiler en general y el de buscar criterios para diseñar instrumentos que permitan utilizar el alquiler en las acciones estatales de vivienda social. Uno de los graves obstáculos que existen actualmente para

  8. PREVALENCIA DE ANORMALIDADES EN LA CITOLOGIA EXOENDOCERVICAL EN UNA POBLACION DE EMBARAZADAS DE POPAYAN 2006

    Microsoft Academic Search

    Luis A. Ospina; María Piedad Acosta; Diego Illera

    Objective: To determine the abnormalities in pap smears found in external ambulatory service to pregnant women in Hospital San Jose , Hospital level II Susana Lopez de Valencia and universitary center health Alfonso Lopez de la Universidad del Cauca in

  9. Aspetti e problemi di una ricerea sinecologica sui Poriferi del Golfo di Napoli

    Microsoft Academic Search

    M. Sarà

    1960-01-01

    L'A. souligue l'importance d'une recherche synécologique comparative sur les éponges, conduite d'une facon organique, et rel'eve l'intérét que la baie de Naples prrsente par sa variété de biotopes. Les biotopes des zones méso., infra. ct circalittorales de ce golfe sont passées apidement en revue, et certains résultats déjà obtenus sont esposés. L'A. passe ensuite à das considérations génerales sur les

  10. La Casa Verde de Mario Vargas Llosa: una imagen del hombre 

    E-print Network

    Frick, Magdalena Capurro

    1976-01-01

    perdurar en eso mundo que obliga a los hombres a despla zarse, a hui. r, a comenzar de nuevo reiteradamente, Lalita, quien sabe que nada que no sea el presente inmediato pue de ser dado por seguro, insiste en que Adrian lieve sus zapatos al partir... obliga a desarraigarse, a huir nuevamente, abandonando lo que habfa logrado construir, Pero la pre sencia de la enfermedad solo acelera un proceso que tar de o temprano se hubiera dado: de no haber abandonado la isla, Fushxa posiblemente hubiera cafdo...

  11. Cambiar una Convención Social Perjudicial: La Ablación o Mutilación Genital Femenina

    Microsoft Academic Search

    2005-01-01

    Sólo en África y Oriente Medio, la práctica de la ablación \\/ mutilación genital femenina (A\\/MGF) viola los derechos humanos de unos tres millones de niñas y mujeres cada año. Mientras tanto, el aumento de la emigración ha hecho recer la preocupación acerca de la A\\/MGF más allá de los países en los que se practicaba tradicionalmente. A pesar el

  12. Caracterización de Klebsiella pneumoniae productora de la ?-lactamasa SHV-5, en una unidad de cuidados intensivos

    Microsoft Academic Search

    Verónica Andrade

    Objective. To perform the molecular characterization of Klebsiella pneumoniae isolates from pediatric patients and health care workers at the intensive care unit of a tertiary care hospital in Mexico City. Material and Methods. Fif- teen Klebsiella pneumoniae isolates collected during an out- break in June 1996 were analyzed; eight were from patients and seven from health care workers of Mexico's

  13. Sistemas de Información en Salud e indicadores de salud: una perspectiva integradora

    Microsoft Academic Search

    Jaume Canela-Soler; David Elvira-Martínez; María Jesús Labordena-Barceló; Enrique Loyola-Elizondo

    2010-01-01

    Health Information Systems (HIS) are the core support to decision-making in health organizations. Within HIS, health indicators (HI) reflect, numerically, events measured in the health-illness continuum. The integrated health information system is intended to standardize, integrate and organize all the information available in health information systems through an accessible and secure repository, and to conveniently distribute information for decision-making.To standardize

  14. Otro monólogo más (D´après Oscar Villegas, Un señor y una señora)

    E-print Network

    Cypess, Sandra M.

    1993-04-01

    Merlin Forster. Glass Onion en vez de comer en la cafetería. Eso es Libertad. Wolff habla de performance como dramaturgo; Matías Montes Huidobro, como crítico, uno de nosotros; pero es dramaturgo, uno de ellos. Bueno, es amigo y basta. Empieza al fin...

  15. Una nina adoptada su conducta en el jardin de infantes (An Adopted Girl's Conduct in Preschool).

    ERIC Educational Resources Information Center

    Truscello de Mason, Marile

    1994-01-01

    Reports observations made by a preschool teacher in Argentina about a four-year-old adopted girl in her preschool. The teacher noticed problems between the girl and her adoptive mother, who tried to prevent the girl from remembering her past. The teacher particularly noticed changes in the girl's drawings. (AA)

  16. Diseno y desarrollo de una base de datos bibliograficos (Design and Development of a Bibliographic Database).

    ERIC Educational Resources Information Center

    Mattenella, L. E.; Velazco, J. W.

    1992-01-01

    This article briefly describes the development of bibliographic retrieval systems in the Instituto de Beneficio di Minerales (IN BE MI) in Salta, Argentina, using the Mini-micro CDS/ISIS software developed by Unesco. (LRW)

  17. Teledermatología diferida: análisis de validez en una serie de 2.000 observaciones

    Microsoft Academic Search

    S. Vañó-Galván; Á. Hidalgo; I. Aguayo-Leiva; M. Gil-Mosquera; L. Ríos-Buceta; M. N. Plana; J. Zamora; A. Martorell-Calatayud; P. Jaén

    2011-01-01

    Background and objectivesThe aim of this study was to assess the validity of store-and-forward teledermatology as a tool to support physicians in primary care and hospital emergency services and reduce the requirement for face-to-face appointments. Diagnostic validity and the approach chosen for patient management (face-to-face vs teledermatology) were compared according to patient origin and diagnostic group.

  18. L'approccio ecosistemico: una proposta innovativa per la gestione della biodiversità e del territorio

    Microsoft Academic Search

    LAURA PADOVANI; PAOLA CARRABBA; FRANCESCO MAURO; UTS Biotecnologie

    A strategy for integrated management of land, water and bio- resources that promotes conservation and sustainable use in a fair and proper way. The concept was approved at the Fifth Conference of the Parties to the Biological Diversity Convention, held in Nairobi in May of 2000. It consists of twelve general principles plus five operating principles to be used in

  19. Exploración del Sistema Solar -- Una mirada hacia el futuro - Duration: 30 seconds.

    NASA Video Gallery

    ¿Piensas que ya sabemos todo acerca de nuestro sistema solar? La realidad es que apenas hemos comenzado con lo que hay para conocer. Únete a la NASA, en el envío de misiones a los confines del sist...

  20. TESTIGOS DE JEHOVÁ Y TRANSFUSIÓN SANGUÍNEA: REFLEXIÓN DESDE UNA ÉTICA NATURAL

    Microsoft Academic Search

    2006-01-01

    RESUMEN El rechazo por parte de los Testigos de Jehová de recibir transfusiones sanguíneas siempre ha representado para los médicos un conflicto ético y un problema en la relación médico paciente. Las razones de este grupo para rechazar las transfusiones se basan en motivos religiosos por un mandato explícito de Dios que se encontraría en la Biblia. Para ellos el