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1

Ribose 5-Phosphate Isomerase Investigations for the Undergraduate Biochemistry Laboratory  

ERIC Educational Resources Information Center

|The enzyme ribose 5-phosphate isomerase (RpiA) has many features that make it attractive as a focal point of a semester-long, advanced biochemistry laboratory for undergraduate students. The protein can easily and inexpensively be isolated from spinach using traditional purification techniques. Characterization of RpiA enzyme activity can be…

Jewett, Kathy; Sandwick, Roger K.

2011-01-01

2

A Curriculum Skills Matrix for Development and Assessment of Undergraduate Biochemistry and Molecular Biology Laboratory Programs  

ERIC Educational Resources Information Center

|We have designed a skills matrix to be used for developing and assessing undergraduate biochemistry and molecular biology laboratory curricula. We prepared the skills matrix for the Project Kaleidoscope Summer Institute workshop in Snowbird, Utah (July 2001) to help current and developing undergraduate biochemistry and molecular biology program…

Caldwell, Benjamin; Rohlman, Christopher; Benore-Parsons, Marilee

2004-01-01

3

Purification and Characterization of Taq Polymerase: A 9-Week Biochemistry Laboratory Project for Undergraduate Students  

ERIC Educational Resources Information Center

|We have developed a 9-week undergraduate laboratory series focused on the purification and characterization of "Thermus aquaticus" DNA polymerase (Taq). Our aim was to provide undergraduate biochemistry students with a full-semester continuing project simulating a research-like experience, while having each week's procedure focus on a single…

Bellin, Robert M.; Bruno, Mary K.; Farrow, Melissa A.

2010-01-01

4

A curriculum skills matrix for development and assessment of undergraduate biochemistry and molecular biology laboratory programs.  

PubMed

We have designed a skills matrix to be used for developing and assessing undergraduate biochemistry and molecular biology laboratory curricula. We prepared the skills matrix for the Project Kaleidoscope Summer Institute workshop in Snowbird, Utah (July 2001) to help current and developing undergraduate biochemistry and molecular biology program designers to determine which laboratory techniques, skills, and theories to include in a 4-year plan. The skills matrix can be used to evaluate and assess the types of laboratory skills as well as the level at which they are taught in biochemistry and molecular biology curricula. The matrix can foster better communication between faculty in chemistry, biology, math, and physics as they share curricular information. As an example of utility of the skills matrix, we used it to survey several commonly used biochemistry laboratory manuals to evaluate the skills covered in each text. PMID:21706681

Caldwell, Benjamin; Rohlman, Christopher; Benore-Parsons, Marilee

2004-01-01

5

Identification of Forensic Samples via Mitochondrial DNA in the Undergraduate Biochemistry Laboratory  

Microsoft Academic Search

A recent forensic approach for identification of unknown biological samples is mitochondrial DNA (mtDNA) sequencing. We describe a laboratory exercise suitable for an undergraduate biochemistry course in which the polymerase chain reaction is used to amplify a 440 base pair hypervariable region of human mtDNA from a variety of \\

Julie T. Millard; André M. Pilon

2003-01-01

6

Forensic Analysis of Canine DNA Samples in the Undergraduate Biochemistry Laboratory  

ERIC Educational Resources Information Center

|Recent advances in canine genomics have allowed the development of highly distinguishing methods of analysis for both nuclear and mitochondrial DNA. We describe a laboratory exercise suitable for an undergraduate biochemistry course in which the polymerase chain reaction is used to amplify hypervariable regions of DNA from dog hair and saliva…

Carson, Tobin M.; Bradley, Sharonda Q.; Fekete, Brenda L.; Millard, Julie T.; LaRiviere, Frederick J.

2009-01-01

7

Identification of Forensic Samples via Mitochondrial DNA in the Undergraduate Biochemistry Laboratory  

NASA Astrophysics Data System (ADS)

A recent forensic approach for identification of unknown biological samples is mitochondrial DNA (mtDNA) sequencing. We describe a laboratory exercise suitable for an undergraduate biochemistry course in which the polymerase chain reaction is used to amplify a 440 base pair hypervariable region of human mtDNA from a variety of "crime scene" samples (e.g., teeth, hair, nails, cigarettes, envelope flaps, toothbrushes, and chewing gum). Amplification is verified via agarose gel electrophoresis and then samples are subjected to cycle sequencing. Sequence alignments are made via the program CLUSTAL W, allowing students to compare samples and solve the "crime."

Millard, Julie T.; Pilon, André M.

2003-04-01

8

HPLC of the Polypeptides in a Hydrolyzate of Egg-White Lysozyme. An Experiment for the Undergraduate Biochemistry Laboratory.  

ERIC Educational Resources Information Center

|Describes a simple high-performance liquid chromatography experiment for undergraduate biochemistry laboratories. The experiment illustrates the separation of polypeptides by a step gradient elution using a single pump instrument with no gradient attachments. Discusses instrumentation, analysis, a sample preparation, and results. (CW)|

Richardson, W. S., III; Burns, L.

1988-01-01

9

HPLC of the Polypeptides in a Hydrolyzate of Egg-White Lysozyme. An Experiment for the Undergraduate Biochemistry Laboratory.  

ERIC Educational Resources Information Center

Describes a simple high-performance liquid chromatography experiment for undergraduate biochemistry laboratories. The experiment illustrates the separation of polypeptides by a step gradient elution using a single pump instrument with no gradient attachments. Discusses instrumentation, analysis, a sample preparation, and results. (CW)

Richardson, W. S., III; Burns, L.

1988-01-01

10

The Determination of Vitamin D-Dependent Calcium Binding Protein in Chick Intesting: An Undergraduate Biochemistry Laboratory Experiment.  

ERIC Educational Resources Information Center

Described is an experiment used in an undergraduate biochemistry laboratory involving inducing rickets in chicks and correlating the disease to a reduction in vitamin D-dependent calcium binding protein. Techniques involved are hormone induction, protein isolation, and radioisotope methodology. (Author/DS)

Lessard, George M.

1980-01-01

11

Glycobiology, How to Sugar-Coat an Undergraduate Advanced Biochemistry Laboratory  

ERIC Educational Resources Information Center

|A second semester biochemistry laboratory has been implemented as an independent projects course at California State University, Sacramento since 1999. To incorporate aspects of carbohydrate biochemistry, or glycobiology, into our curriculum, projects in lectin isolation and purification were undertaken over the course of two semesters. Through…

McReynolds, Katherine D.

2006-01-01

12

Purification of Bovine Carbonic Anhydrase by Affinity Chromatography: An Undergraduate Biochemistry Laboratory Experiment  

NASA Astrophysics Data System (ADS)

We have developed a rapid and inexpensive experiment utilizing affinity chromatography to isolate carbonic anhydrase (CA) from bovine blood. The more specific an affinity gel is the better the purification, but the greater the cost. Some costs would be prohibitive in the undergraduate biochemistry laboratory. Less specific resins may be more affordable but may bind a number of closely related proteins. One alternative would be to couple a specific ligand to an inexpensive resin such as an ion exchanger. We describe a simple procedure for preparing a sulfonamide-coupled resin which specifically binds CA from a blood hemolysate. The CA is eluted and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It was found that only a single band of 31 kD was obtained. The instructor can readily prepare the affinity gel prior to the lab, and the students, beginning with packed red blood cells can carry out the lysis, binding to the gel, elution, enzymatic assays, and electrophoresis.

Bering, C. Larry; Kuhns, Jennifer J.; Rowlett, Roger

1998-08-01

13

Affinity Chromatography of Lactate Dehydrogenase: An Experiment for the Undergraduate Biochemistry Laboratory.  

ERIC Educational Resources Information Center

|Discusses a laboratory technique of enzyme purification by affinity chromatography as part of an undergraduate biochemical methodology course. Provides preparation details of the rat muscle homogenate and reagents. Proposes column requirements and assaying information. (MVL)|

Anderson, Alexander J.

1988-01-01

14

Affinity Chromatography of Lactate Dehydrogenase: An Experiment for the Undergraduate Biochemistry Laboratory.  

ERIC Educational Resources Information Center

Discusses a laboratory technique of enzyme purification by affinity chromatography as part of an undergraduate biochemical methodology course. Provides preparation details of the rat muscle homogenate and reagents. Proposes column requirements and assaying information. (MVL)

Anderson, Alexander J.

1988-01-01

15

A Static Method as an Alternative to Gel Chromatography: An Experiment for the Undergraduate Biochemistry Laboratory  

ERIC Educational Resources Information Center

|This article describes a static method as an alternative to gel chromatography, which may be used as an undergraduate laboratory experiment. In this method, a constant mass of Sephadex gel is swollen in a series of protein solutions. UV-vis spectrophotometry is used to find a partition coefficient, KD, that indicates the fraction of the interior…

Burum, Alex D.; Splittgerber, Allan G.

2008-01-01

16

An Inexpensive, Relatively Green, and Rapid Method to Purify Genomic DNA from "Escherichia Coli": An Experiment for the Undergraduate Biochemistry Laboratory  

ERIC Educational Resources Information Center

|A method to purify genomic DNA from "Escherichia coli" is presented. The method is an amalgam of published methods but has been modified and optimized for use in the undergraduate biochemistry laboratory. Specifically, the method uses Tide Free 2x Ultra laundry detergent, which contains unspecified proteases and lipases, "n"-butanol, 2-propanol,…

Sims, Paul A.; Branscum, Katie M.; Kao, Lydia; Keaveny, Virginia R.

2010-01-01

17

Environmental regulation of plant gene expression: An RT-qPCR laboratory project for an upper-level undergraduate biochemistry or molecular biology course.  

PubMed

We present a novel laboratory project employing "real-time" RT-qPCR to measure the effect of environment on the expression of the FLOWERING LOCUS C gene, a key regulator of floral timing in Arabidopsis thaliana plants. The project requires four 3-hr laboratory sessions and is aimed at upper-level undergraduate students in biochemistry or molecular biology courses. The project provides students with hands-on experience with RT-qPCR, the current "gold standard" for gene expression analysis, including detailed data analysis using the common 2-??CT method. Moreover, it provides a convenient starting point for many inquiry-driven projects addressing diverse questions concerning ecological biochemistry, naturally occurring genetic variation, developmental biology, and the regulation of gene expression in nature. © 2013 by The International Union of Biochemistry and Molecular Biology, 41(5):325-333, 2013. PMID:24038665

Eickelberg, Garrett J; Fisher, Alison J

2013-09-10

18

Equilibrium Gel Filtration Chromatography for the Measurement of Protein-Ligand Binding in the Undergraduate Biochemistry Laboratory  

ERIC Educational Resources Information Center

|A laboratory exercise used in the senior biochemistry course at the University of Winnipeg for three years is discussed. It combines liquid chromatography and absorbance spectroscopy and also allows the students to produce a quantitative result within a single three-hour period.|

Craig, Douglas B.

2005-01-01

19

A Kinetic Experiment for the Biochemistry Laboratory.  

ERIC Educational Resources Information Center

|Discusses the use of specific reactions of metabolic pathways to make measurements in the laboratory. Describes an adaptation of an experiment used in undergraduate biochemistry laboratories involving the induction of an enzyme in E. coli, as well as its partial purification and characterization. (TW)|

Palmer, Richard E.

1986-01-01

20

An Integrated Biochemistry Laboratory, Including Molecular Modeling  

Microsoft Academic Search

The dilemma of designing an advanced undergraduate laboratory lies in the desire to teach and reinforce basic principles and techniques while at the same time exposing students to the excitement of research. We report here on a one-semester, project-based biochemistry laboratory that combines the best features of a cookbook approach (high success rate, achievement of defined goals) with those of

Adele J. Wolfson Mona L. Hall; Thomas R. Branham

1996-01-01

21

The Biochemistry of the Muscle Contraction Process: An Undergraduate Laboratory Experiment Using Viscosity to Follow the Progress of a Reaction.  

ERIC Educational Resources Information Center

|Describes an undergraduate laboratory experiment using viscosity to follow the progress of the contractile process in muscles. This simple, short experiment illustrates the action of ATP as the source of energy in the contractile process and the catalytic effect of calcium ions as a control in the energy producing process. (CS)|

Belliveau, James F.; And Others

1981-01-01

22

The Biochemistry of the Muscle Contraction Process: An Undergraduate Laboratory Experiment Using Viscosity to Follow the Progress of a Reaction.  

ERIC Educational Resources Information Center

Describes an undergraduate laboratory experiment using viscosity to follow the progress of the contractile process in muscles. This simple, short experiment illustrates the action of ATP as the source of energy in the contractile process and the catalytic effect of calcium ions as a control in the energy producing process. (CS)

Belliveau, James F.; And Others

1981-01-01

23

Immobilized alpha-Galactosidase in the Biochemistry Laboratory  

ERIC Educational Resources Information Center

|This laboratory experiment was designed to demonstrate the application of immobilized galactosidase in food industry to hydrolyze raffinose family oligosaccharides in soymilk. This laboratory experiment was conducted for postgraduate students of biochemistry and developed for graduate and undergraduate students of biochemistry, biotechnology,…

Mulimani, V. H.; Dhananjay, K.

2007-01-01

24

Assessment of Molecular Construction in Undergraduate Biochemistry  

ERIC Educational Resources Information Center

White and group used a two question, open-ended tests to separately evaluate students' learning of specific biochemical concepts in the general biology lecture and laboratory, in the first performance assessment of molecular visualization in teaching biochemistry. Two studies were devoted to protein structure using globins followed by one…

Booth, Deborah; Bateman, Robert C., Jr.; Sirochman, Rudy; Richardson, David C.; Richardson, Jane S.; Weiner, Steven W.; Farwell, Mary; Putnam-Evans, Cindy

2005-01-01

25

Assessment of Molecular Construction in Undergraduate Biochemistry  

ERIC Educational Resources Information Center

|White and group used a two question, open-ended tests to separately evaluate students' learning of specific biochemical concepts in the general biology lecture and laboratory, in the first performance assessment of molecular visualization in teaching biochemistry. Two studies were devoted to protein structure using globins followed by one…

Booth, Deborah; Bateman, Robert C., Jr.; Sirochman, Rudy; Richardson, David C.; Richardson, Jane S.; Weiner, Steven W.; Farwell, Mary; Putnam-Evans, Cindy

2005-01-01

26

Cogenerative Dialogue: Improving Undergraduate Biochemistry Teaching and Learning  

Microsoft Academic Search

Students entering undergraduate biochemistry courses are usually already committed to studies in biochemistry or a related field. These students have excelled, in general, in biology, organic chemistry, and physics before starting the biochemistry two-semester sequence. Most of the publications on college science teaching focus on the introductory science courses, and few and far between are the publications on upper-level science

Penny J. Gilmer; Mohammed Al-humiari; Donald D. Bratton

2008-01-01

27

An "in Silico" DNA Cloning Experiment for the Biochemistry Laboratory  

ERIC Educational Resources Information Center

|This laboratory exercise introduces students to concepts in recombinant DNA technology while accommodating a major semester project in protein purification, structure, and function in a biochemistry laboratory for junior- and senior-level undergraduate students. It is also suitable for forensic science courses focused in DNA biology and advanced…

Elkins, Kelly M.

2011-01-01

28

Incorporation of bioinformatics exercises into the undergraduate biochemistry curriculum  

Microsoft Academic Search

The field of bioinformatics is developing faster than most biochemistry textbooks can adapt. Supplement- ing the undergraduate biochemistry curriculum with data-mining exercises is an ideal way to expose the students to the common databases and tools that take advantage of this vast repository of biochemical information. An integrated collection of exercises based on pet proteins has been assembled. The exer-

Andrew L. Feig; Evelyn Jabri

2002-01-01

29

Incorporation of Bioinformatics Exercises into the Undergraduate Biochemistry Curriculum  

ERIC Educational Resources Information Center

The field of bioinformatics is developing faster than most biochemistry textbooks can adapt. Supplementing the undergraduate biochemistry curriculum with data-mining exercises is an ideal way to expose the students to the common databases and tools that take advantage of this vast repository of biochemical information. An integrated collection of…

Feig, Andrew L.; Jabri, Evelyn

2002-01-01

30

An Undergraduate Investigation into the 10-23 DNA Enzyme that Cleaves RNA: DNA Can Cut It in the Biochemistry Laboratory  

ERIC Educational Resources Information Center

|A low-cost biochemistry experiment is described that demonstrates current techniques in the use of catalytic DNA molecules and introduces a nonradioactive, nonfluorescent, inexpensive, fast, and safe method for monitoring these nucleic acid reactions. The laboratory involves the exploration of the 10-23 DNA enzyme as it cleaves a specific RNA…

Flynn-Charlebois, Amber; Burns, Jamie; Chapelliquen, Stephanie; Sanmartino, Holly

2011-01-01

31

Immobilized Lactase in the Biochemistry Laboratory  

NASA Astrophysics Data System (ADS)

Immobilized enzymes have many practical applications. They may be used in clinical, industrial, and biotechnological laboratories and in many clinical diagnostic kits. For educational purposes, use of immobilized enzymes can easily be taught at the undergraduate or even secondary level. We have developed an immobilized enzyme experiment that combines many practical techniques used in the biochemistry laboratory and fits within a three-hour time frame. In this experiment, lactase from over-the-counter tablets for patients with lactose intolerance is immobilized in polyacrylamide, which is then milled into small beads and placed into a chromatography column. A lactose solution is added to the column and the eluant is assayed using the glucose oxidase assay, available as a kit. We have determined the optimal conditions to give the greatest turnover of lactose while allowing the immobilized enzymes to be active for long periods at room temperature.

Allison, Matthew J.; Bering, C. Larry

1998-10-01

32

Differentiating Biochemistry Course Laboratories Based on Student Experience  

ERIC Educational Resources Information Center

|Content and emphases in undergraduate biochemistry courses can be readily tailored to accommodate the standards of the department in which they are housed, as well as the backgrounds of the students in the courses. A more challenging issue is how to construct laboratory experiences for a class with both chemistry majors, who usually have little…

Jakubowski, Henry V.

2011-01-01

33

Biochemistry in Undergraduate Health Courses: Structure and Organization  

ERIC Educational Resources Information Center

|This article describes the following aspects of teaching biochemistry in undergraduate health courses: objectives, number of hours, time in which the subject is studied, selection of content, teaching strategies, and evaluation methodologies used. Fifty-three courses distributed in 13 areas within the health field and offered by 12 institutions…

Silva, Irani F.; Batista, Nildo A.

2003-01-01

34

A Streamlined Molecular Biology Module for Undergraduate Biochemistry Labs  

ERIC Educational Resources Information Center

|Site-directed mutagenesis and other molecular biology techniques, including plasmid manipulation and restriction analysis, are commonly used tools in the biochemistry research laboratory. In redesigning our biochemistry lab curricula, we sought to integrate these techniques into a term-long, project-based course. In the module presented here,…

Muth, Gregory W.; Chihade, Joseph W.

2008-01-01

35

Raising environmental awareness through applied biochemistry laboratory experiments.  

PubMed

Our environment is under constant pressure and threat from various sources of pollution. Science students, in particular chemistry students, must not only be made aware of these issues, but also be taught that chemistry (and science) can provide solutions to such real-life issues. To this end, a newly developed biochemistry laboratory experiment is described that guides students to learn about the applicability of peroxidase enzymes to degrade organic dyes (as model pollutants) in simulated waste water. In addition to showing how enzymes can potentially be used for waste water remediation, various factors than can affect enzyme-based reactions such as pH, temperature, concentration of substrates/enzymes, and denaturants can also be tested. This "applied biotechnology" experiment was successfully implemented in an undergraduate biochemistry laboratory course to enhance students' learning of environmental issues as well important biochemistry concepts. Student survey confirmed that this laboratory experiment was successful in achieving the objectives of raising environmental awareness in students and illustrating the usefulness of chemistry in solving real-life problems. This experiment can be easily adopted in an introductory biochemistry laboratory course and taught as an inquiry-guided exercise. © 2013 by The International Union of Biochemistry and Molecular Biology, 41(5):341-347, 2013. PMID:24078356

Salman Ashraf, S

2013-09-01

36

An Integrated Biochemistry Laboratory, Including Molecular Modeling  

NASA Astrophysics Data System (ADS)

The dilemma of designing an advanced undergraduate laboratory lies in the desire to teach and reinforce basic principles and techniques while at the same time exposing students to the excitement of research. We report here on a one-semester, project-based biochemistry laboratory that combines the best features of a cookbook approach (high success rate, achievement of defined goals) with those of an investigative, discovery-based approach (student involvement in the experimental design, excitement of real research). Individual modules may be selected and combined to meet the needs of different courses and different institutions. The central theme of this lab is protein purification and design. This laboratory accompanies the first semester of biochemistry (Structure and Function of Macromolecules, a course taken mainly by junior and senior chemistry and biological chemistry majors). The protein chosen as the object of study is the enzyme lysozyme, which is utilized in all projects. It is suitable for a student lab because it is easily and inexpensively obtained from egg white and is extremely stable, and its high isoelectric point (pI = 11) allows for efficient separation from other proteins by ion-exchange chromatography. Furthermore, a literature search conducted by the resourceful student reveals a wealth of information, since lysozyme has been the subject of numerous studies. It was the first enzyme whose structure was determined by crystallography (1). Hendrickson et al. (2) have previously described an intensive one-month laboratory course centered around lysozyme, although their emphasis is on protein stability rather than purification and engineering. Lysozyme continues to be the focus of much exciting new work on protein folding and dynamics, structure and activity (3 - 5). This lab course includes the following features: (i) reinforcement of basic techniques, such as preparation of buffers, simple enzyme kinetics, and absorption spectroscopy; (ii) experience with methods of protein purification; (iii) incorporation of appropriate controls into experiments; (iv) use of basic statistics in data analysis; (v) writing papers and grant proposals in accepted scientific style; (vi) peer review; (vii) oral presentation of results and proposals; and (viii) introduction to molecular modeling. Figure 1 illustrates the modular nature of the lab curriculum. Elements from each of the exercises can be separated and treated as stand-alone exercises, or combined into short or long projects. We have been able to offer the opportunity to use sophisticated molecular modeling in the final module through funding from an NSF-ILI grant. However, many of the benefits of the research proposal can be achieved with other computer programs, or even by literature survey alone. Figure 1.Design of project-based biochemistry laboratory. Modules (projects, or portions of projects) are indicated as boxes. Each of these can be treated independently, or used as part of a larger project. Solid lines indicate some suggested paths from one module to the next. The skills and knowledge required for protein purification and design are developed in three units: (i) an introduction to critical assays needed to monitor degree of purification, including an evaluation of assay parameters; (ii) partial purification by ion-exchange techniques; and (iii) preparation of a grant proposal on protein design by mutagenesis. Brief descriptions of each of these units follow, with experimental details of each project at the end of this paper. Assays for Lysozyme Activity and Protein Concentration (4 weeks) The assays mastered during the first unit are a necessary tool for determining the purity of the enzyme during the second unit on purification by ion exchange. These assays allow an introduction to the concept of specific activity (units of enzyme activity per milligram of total protein) as a measure of purity. In this first sequence, students learn a turbidimetric assay for lysozyme activity and a colorimetric one for protein concentration. Familiarity with the assay

Hall, Adele J. Wolfson Mona L.; Branham, Thomas R.

1996-11-01

37

Assessing Undergraduate Laboratory Performance  

NSDL National Science Digital Library

The lab notebook is one element for assessing student laboratory performance. However, it is also important to be able to communicate research results in a journal article format and a visual poster format. Another key aspect to scientific research is the ability to present a research plan. This article describes four assessment tools that can be used in conjunction with undergraduate lab courses that provide the opportunity for the students to practice each of these essential communication skills.

Elizabeth Adler (AAAS;); Nancy Gough (AAAS;)

2006-09-05

38

A Project-Oriented Biochemistry Laboratory Course  

NASA Astrophysics Data System (ADS)

A project-oriented laboratory course has been designed to introduce students to the study of biochemistry as it is practiced. The course is designed to be a capstone experience for students enrolled in a variety of majors at the Rochester Institute of Technology, including those who enter our new B.S. Biochemistry program. The experiments in this course enable the students to explore the protein chemistry, enzymology, and molecular biology of a single enzyme, threonine dehydrogenase, in a series of integrated experiments. The laboratory incorporates both traditional methods (centrifugation, UV-vis spectroscopy, gel electrophoresis, and chromatography) and more recent developments in the field (polymerase chain reaction). Students use a small computer network to prepare for experiments (using simulation software developed at RIT), to evaluate data, to access sequence homology databases over the Internet, and to visualize and model proteins and nucleic acids. The change in the biochemistry teaching lab from a sequence of unrelated experiments to an integrated series of experiments is a model that can be readily adapted by other educators, who can change their courses to focus on a single enzyme with which they are most familiar.

Craig, Paul A.

1999-08-01

39

The Kinetics and Inhibition of Gamma-Glutamyl Transpeptidase: A Biochemistry Laboratory Experiment.  

ERIC Educational Resources Information Center

|Discusses an enzyme kinetics laboratory experiment involving a two substrate system for undergraduate biochemistry. Uses the enzyme gamma-glutamyl transpeptidase as this enzyme in blood serum is of clinical significance. Notes elevated levels are seen in liver disease, alcoholism, and epilepsy. Uses a spectrophotometer for the analysis. (MVL)|

Splittgerber, A. G.; Sohl, Julie

1988-01-01

40

Thermodynamic Exploration of Eosin-Lysozyme Binding: A Physical Chemistry and Biochemistry Laboratory Experiment  

ERIC Educational Resources Information Center

We developed a modular pair of experiments for use in the undergraduate physical chemistry and biochemistry laboratories. Both experiments examine the thermodynamics of the binding of a small molecule, eosin Y, to the protein lysozyme. The assay for binding is the quenching of lysozyme fluorescence by eosin through resonant energy transfer. In…

Huisman, Andrew J.; Hartsell, Lydia R.; Krueger, Brent P.; Pikaart, Michael J.

2010-01-01

41

Kinetics of Carboxylesterase: An Experiment for Biochemistry and Physical Chemistry Laboratory.  

ERIC Educational Resources Information Center

|Describes a convenient, inexpensive experiment in enzyme kinetics developed for the undergraduate biochemistry laboratory at the University of Virginia. Required are a single beam visible spectrophotometer with output to a recorder, a constant temperature, a commercially available enzyme, substrates, and buffers. (BT)|

Nichols, C. S.; Cromartie, T. H.

1979-01-01

42

An SDS-PAGE Examination of Protein Quaternary Structure and Disulfide Bonding for a Biochemistry Laboratory  

ERIC Educational Resources Information Center

|Electrophoresis is a valuable tool for biochemists, yet this technique is often not included in biochemistry laboratory curricula owing to time constraints or lack of equipment. Protein structure is also a topic of interest in many disciplines, yet most undergraduate lab experiments focus only on primary structure. In this experiment, students…

Powers, Jennifer L.; Andrews, Carla S.; St. Antoine, Caroline C.; Jain, Swapan S.; Bevilacqua, Vicky L. H.

2005-01-01

43

Integrating Internet Assignments into a Biochemistry/Molecular Biology Laboratory Course  

ERIC Educational Resources Information Center

|A main challenge in educating undergraduate students is to introduce them to the Internet and to teach them how to effectively use it in research. To this end, an Internet assignment was developed that introduces students to websites related to biomedical research at the beginning of a biochemistry/molecular biology laboratory course. The basic…

Kaspar, Roger L.

2002-01-01

44

Integrating Internet Assignments into a Biochemistry/Molecular Biology Laboratory Course  

ERIC Educational Resources Information Center

A main challenge in educating undergraduate students is to introduce them to the Internet and to teach them how to effectively use it in research. To this end, an Internet assignment was developed that introduces students to websites related to biomedical research at the beginning of a biochemistry/molecular biology laboratory course. The basic…

Kaspar, Roger L.

2002-01-01

45

The Kinetics and Inhibition of Gamma-Glutamyl Transpeptidase: A Biochemistry Laboratory Experiment.  

ERIC Educational Resources Information Center

Discusses an enzyme kinetics laboratory experiment involving a two substrate system for undergraduate biochemistry. Uses the enzyme gamma-glutamyl transpeptidase as this enzyme in blood serum is of clinical significance. Notes elevated levels are seen in liver disease, alcoholism, and epilepsy. Uses a spectrophotometer for the analysis. (MVL)

Splittgerber, A. G.; Sohl, Julie

1988-01-01

46

Kinetics of Carboxylesterase: An Experiment for Biochemistry and Physical Chemistry Laboratory.  

ERIC Educational Resources Information Center

Describes a convenient, inexpensive experiment in enzyme kinetics developed for the undergraduate biochemistry laboratory at the University of Virginia. Required are a single beam visible spectrophotometer with output to a recorder, a constant temperature, a commercially available enzyme, substrates, and buffers. (BT)

Nichols, C. S.; Cromartie, T. H.

1979-01-01

47

Thermodynamic Exploration of Eosin-Lysozyme Binding: A Physical Chemistry and Biochemistry Laboratory Experiment  

ERIC Educational Resources Information Center

|We developed a modular pair of experiments for use in the undergraduate physical chemistry and biochemistry laboratories. Both experiments examine the thermodynamics of the binding of a small molecule, eosin Y, to the protein lysozyme. The assay for binding is the quenching of lysozyme fluorescence by eosin through resonant energy transfer. In…

Huisman, Andrew J.; Hartsell, Lydia R.; Krueger, Brent P.; Pikaart, Michael J.

2010-01-01

48

78 FR 4170 - License Amendment Request for Analytical Bio-Chemistry Laboratories, Inc., Columbia, MO  

Federal Register 2010, 2011, 2012, 2013

...Amendment Request for Analytical Bio-Chemistry Laboratories, Inc., Columbia, MO...24-13365-01 issued to Analytical Bio-Chemistry Laboratories, Inc. (the licensee...accession numbers are: 1. Analytical Bio-Chemistry Laboratories, Inc., Licensee...

2013-01-18

49

Teaching structure: Student use of software tools for understanding macromolecular structure in an undergraduate biochemistry course.  

PubMed

Because understanding the structure of biological macromolecules is critical to understanding their function, students of biochemistry should become familiar not only with viewing, but also with generating and manipulating structural representations. We report a strategy from a one-semester undergraduate biochemistry course to integrate use of structural representation tools into both laboratory and homework activities. First, early in the course we introduce the use of readily available open-source software for visualizing protein structure, coincident with modules on amino acid and peptide bond properties. Second, we use these same software tools in lectures and incorporate images and other structure representations in homework tasks. Third, we require a capstone project in which teams of students examine a protein-nucleic acid complex and then use the software tools to illustrate for their classmates the salient features of the structure, relating how the structure helps explain biological function. To ensure engagement with a range of software and database features, we generated a detailed template file that can be used to explore any structure, and that guides students through specific applications of many of the software tools. In presentations, students demonstrate that they are successfully interpreting structural information, and using representations to illustrate particular points relevant to function. Thus, over the semester students integrate information about structural features of biological macromolecules into the larger discussion of the chemical basis of function. Together these assignments provide an accessible introduction to structural representation tools, allowing students to add these methods to their biochemical toolboxes early in their scientific development. © 2013 by The International Union of Biochemistry and Molecular Biology, 41(5):351-359, 2013. PMID:24019219

Jaswal, Sheila S; O'Hara, Patricia B; Williamson, Patrick L; Springer, Amy L

2013-09-10

50

Synthesis of Vitamin K Expoxide: An Undergraduate Biochemistry Experiment.  

ERIC Educational Resources Information Center

|Provides procedures for synthesizing and purifying a vitamin K metabolite (2,3-epoxide) to introduce many of the techniques used in lipid biochemistry. Includes typical results obtained as well as an optional experiment designed to test the purity of the epoxide obtained. (JM)|

Thierry-Palmer, M.

1984-01-01

51

Undergraduate Laboratory for Surface Science  

NASA Astrophysics Data System (ADS)

Surface science has developed into a multidisciplinary field of research with applications ranging from heterogeneous catalysis to semiconductor etching (1). Aspects of surface chemistry are now included in physical chemistry textbooks (2) and undergraduate curricula (3), but the perceived cost and complexity of equipment has deterred the introduction of surface science methods in undergraduate laboratories (4). Efforts to expose chemistry undergraduates to state-of-the-art surface instrumentation have just begun (5). To provide our undergraduates with hands-on experience in using standard techniques for characterizing surface morphology, adsorbates, kinetics, and reaction mechanisms, we have developed a set of surface science experiments for our physical chemistry laboratory sequence. The centerpiece of the laboratory is an ultrahigh vacuum (UHV) chamber for studies of single crystal surfaces. This instrument, shown in the figure, has surface analysis capabilities including low energy electron diffraction (LEED), Auger spectroscopy, and temperature-programmed desorption (TPD). The laboratory exercises involve experiments on the well-studied Pt(111) surface. Students prepare a previously mounted single crystal sample by sputtering it with an argon ion gun and heating it under O2. Electron diffraction patterns from the cleaned surface are then obtained with a reverse view LEED apparatus (Princeton Instruments). Images are captured by a charge-coupled device (CCD) camera interfaced to a personal computer for easy downloading and subsequent analysis. Although the LEED images from a Pt(111) surface can be readily interpreted using simple diffraction arguments, this lab provides an excellent context for introducing Miller indices and reciprocal lattices (6). The surface chemical composition can be investigated by Auger spectroscopy, using the LEED apparatus as a simple energy analyzer. The temperature programmed desorption experiment, which is nearly complete, will be be added to the curriculum this academic year. This experiment introduces students to the concepts of surface adsorption and desorption kinetics. The sample mount can be both heated to 900 oC and cooled by liquid nitrogen, allowing the study of the desorption of a variety of adsorbates, both chemisorbed and physisorbed. Adsorbed species evolving from the heated surface are detected with a quadrupole mass spectrometer. Initially, students will study the desorption kinetics of CO from a Pt(111) surface for a range of coverages and temperature programming rates in order to obtain rate parameters and to test the validity of Redhead's relationship between the activation energy of desorption and the peak desorption temperature. They then will be introduced to the mechanisms of surface reactions (Langmuir-Hinshelwood) in a study of CO oxidation on this surface. We have also set up a scanning tunneling microscopy (STM) laboratory using a commercially available instrument (Burleigh Instruments, Inc.), which complements the UHV surface structure experiments by introducing the topography of a real surface, for example, with steps and defect sites. With the apparatus now completed, we can explore other possible developments, for example, an applied physics track designed around experiments on semiconductor substrates. Future additions include X-ray photoelectron spectroscopy and completion of a separate surface infrared spectroscopy experiment on supported catalysts. We acknowledge support from the National Science Foundation, Division of Undergraduate Education Instrumentation and Laboratory Improvement (ILI) Program (Grant No. DUE-9352254) and an AT&T Special Purpose Grant. Literature Cited Somorjai, G. Introduction to Surface Chemistry and Catalysis; Wiley: New York, 1994; Woodruff, D. P.; Delchar, T. A. Modem Techniques of Surface Science; Cambridge University: Cambridge, 1986; Christmann, K. Introduction to Surface Physical Chemistry; Springer Verlag: New York, 1991; Adamson, A. W. Physical Chemistry of Surfaces, 5th ed.; Wiley: New York, 1990. See for example Atki

Okumura, Mitchio; Beauchamp, Jesse L.; Dickert, Jeffrey M.; Essy, Blair R.; Claypool, Christopher L.

1996-02-01

52

A Laboratory Course in Clinical Biochemistry Emphasizing Interest and Relevance  

ERIC Educational Resources Information Center

|Ten laboratory experiments are described which are used in a successful clinical biochemistry laboratory course (e.g. blood alcohol, glucose tolerance, plasma triglycerides, coronary risk index, gastric analysis, vitamin C and E). Most of the experiments are performed on the students themselves using simple equipment with emphasis on useful…

Schwartz, Peter L.

1975-01-01

53

Biochemistry of Neuromuscular Diseases: A Course for Undergraduate Students  

ERIC Educational Resources Information Center

|This article outlines an undergraduate course focusing on supramolecular membrane protein complexes involved in the molecular pathogenesis of neuromuscular disorders. The emphasis of this course is to introduce students to the key elements involved in the ion regulation and membrane stabilization during muscle contraction and the role of these…

Ohlendieck, Kay

2002-01-01

54

Industrial Approach to Undergraduate Polymer Laboratory.  

ERIC Educational Resources Information Center

Describes an undergraduate polymer laboratory program in which the laboratory simulates an industrial organization. The goals of the program are to foster an understanding of the properties of polymers and how they change, their preparation, and industrial application. (JN)

Staskiewicz, Bernard A.; And Others

1984-01-01

55

A course designed for undergraduate biochemistry students to learn about cultural diversity issues.  

PubMed

Biology, biochemistry, and other science students are well trained in science and familiar with how to conduct and evaluate scientific experiments. They are less aware of cultural issues or how these will impact their careers in research, education, or as professional health care workers. A course was developed for advanced undergraduate science majors to learn about diversity issues in a context that would be relevant to them, entitled "Diversity Issues in Health Care: Treatment and Research." Learning objectives included: developing awareness of current topics concerning diversity issues in health care; learning how research is carried out in health care, including pharmaceutical research, clinical trials, and social research; and learning about health care practices. Lectures and projects included readings on laboratory and clinical research, as well as literature on legal, race, gender, language, age, and income issues in health care research and clinical practice. Exams, papers, and a service learning project were used to determine the final course grade. Assessment indicated student understanding of diversity issues was improved, and the material was relevant. PMID:21638709

Benore-Parsons, Marilee

2006-09-01

56

Argumentation in undergraduate chemistry laboratories  

NASA Astrophysics Data System (ADS)

To address the need for reform in undergraduate science education a new instructional model called Argument-Driven Inquiry (ADI) was developed and then implemented in a undergraduate chemistry course at a community college in the southeastern United States (Sampson, Walker, & Grooms, 2009; Walker, Sampson, & Zimmerman, in press). The ADI instructional model is designed to give a more central place to argumentation and the role of argument in the social construction of scientific knowledge. This research investigated the growth in the quality of the student generated arguments and the scientific argumentation that took place over the course of a semester. Students enrolled in two sections of General Chemistry I laboratory at the community college participated in this study. The students worked in collaborative groups of three or four. The students were given a variation of the same performance task three times during the semester in order to measure individual ability to use evidence and justify their choice of evidence with appropriate rationale. Five ADI investigations took place during the semester and the laboratory reports for each were collected from each student and the argument section of each report was scored. All the student groups were video recorded five times during the semester as they generated and evaluated arguments and the quality of the group argumentation was assessed using an instrument called the Assessment of Scientific Argumentation in the Classroom (ASAC) observation protocol. As time was the independent variable in this study a repeated measure ANOVA was used to evaluate the significance of student improvement in each area (argumentation, written argument and performance task) over the course of the semester (Trochim, 1999). In addition, a multiple regression analysis was conducted to evaluate how well the ASAC scores predicted individual scores on both the performance task and the written arguments (Green & Salkind, 2005). There was significant growth over the course of the semester in all three measures, performance-based assessment, written argument and oral argumentation. There also was a significant correlation between written and oral arguments that was used to generate a linear model using oral argumentation as a predictor of written argument. The results of this suggest that the use of an integrated instructional model such as ADI can have a positive impact on the quality of the arguments students include in their investigation reports, the argumentation they engage in during lab activities, and their overall performance on tasks that require them to develop and support a valid conclusion with genuine evidence.

Walker, Joi Phelps

57

Teaching Writing Skills in the Undergraduate Laboratory  

Microsoft Academic Search

Stanford University's Writing in the Major program (WIM) requires every undergraduate degree program to include a writing course specific to its field of study. In the physics department's WIM course, undergraduates learn writing skills by composing laboratory reports in the form of journal articles. While studying such topics as scintillation and population inversion, students also practice techniques for communicating the

Dennis W. Ugolini

1998-01-01

58

Fluid Flow Experiment for Undergraduate Laboratory.  

ERIC Educational Resources Information Center

|The undergraduate fluid mechanics laboratory at Clarkson University consists of three experiments: mixing; drag measurements; and fluid flow and pressure drop measurements. The latter experiment is described, considering equipment needed, procedures used, and typical results obtained. (JN)|

Vilimpochapornkul, Viroj; Obot, Nsima T.

1986-01-01

59

Mass Spectrometry for Large Undergraduate Laboratory Sections  

Microsoft Academic Search

Mass spectrometry is routinely covered in undergraduate organic chemistry courses and a number of valuable laboratory experiments featuring its use have been discussed (1-7). Although such experiments work well at institutions with limited laboratory enrollments, we typically teach laboratories with enrollments of 160 or more in which it is difficult to allow each student to carry out a meaningful \\

A. Illies; P. B. Shevlin; G. Childers; M. Peschke; J. Tsai

1995-01-01

60

A survey validation and analysis of undergraduate medical biochemistry practical curriculum in maharashtra, India.  

PubMed

In order to review the strengths and weaknesses of medical biochemistry practical curriculum for undergraduates and to generate ideas to improve it, a questionnaire was sent to 50 biochemistry faculty members selected (through simple random sampling method) from 42 medical colleges of Maharashtra, India. 39 responded to the questionnaire, representing a 78% response rate. The internal consistency of the questionnaire sections was found to be satisfactory (>0.7). The respondents did not agree that the ongoing curriculum was in alignment with learning outcomes (8%), that it encouraged active learning (28%), helped to apply knowledge to clinical situations (18%) and promoted critical thinking and problem solving skills (28%). There were a number of qualitative experiments that were rated 'irrelevant'. Qualitative and quantitative experiments related to recent advances were suggested to be introduced by the respondents. Checklists for the practicals and new curriculum objectives provided in the questionnaire were also approved. The results of the curriculum evaluation suggest a need for re-structuring of practical biochemistry curriculum and introduction of a modified curriculum with more clinical relevance. PMID:23277713

Dandekar, Sucheta P; Maksane, Shalini N; McKinley, Danette

2011-11-08

61

Biochemistry  

NSDL National Science Digital Library

This Web site is the online companion to the fifth edition of Biochemistry, (W. H. Freeman & Co publisher). The site "is designed to help students review key concepts from the textbook through interactive exercises, animated 3D tutorials and learning tools." Most of these amazing resources, including animated flashcards and experimental techniques, are immediately available by clicking on chapter or resource headings. Additional resources, such as the online quizzing feature, require a free online registration (teachers must verify their instructor status).

2002-01-01

62

Mössbauer Spectroscopy in the Undergraduate Laboratory  

NASA Astrophysics Data System (ADS)

A Mössbauer spectrometer has been built primarily from materials that are already available in the physics department at Weber State University. These materials are either common to undergraduate labs or accessible to a small budget, making reproduction at other establishments very feasible. The spectrometer is designed to illustrate and facilitate understanding of physics principles associated with Mössbauer spectroscopy, such as Doppler broadening, isomer shift, recoilless gamma ray emission, and resonant absorption. Ultimately the spectrometer will be incorporated into a junior-level physics laboratory course for physics majors. In practice, the lab work is based around the 14.4 keV gamma ray emitted by Co-57 common to Mössbauer spectroscopy. In addition to serving as an undergraduate teaching laboratory, design improvements in the near future will enable meaningful undergraduate research to be carried out. As a proof of principle, we present a preliminary Mössbauer spectrum and a measurement of the isomer shift in a stainless steel sample.

Decaria, Adam; Hatch, Spencer; Inglefield, Colin

2011-10-01

63

Simple vacuum experiments for undergraduate student laboratories  

Microsoft Academic Search

An overview of typical simple experiments, intended for didactic laboratory vacuum classes for undergraduate students majoring in Physics, Physics Engineering and Material Sciences, is presented.For all the experiments, the students perform actual measurements of characteristics of different vacuum components or material properties and compare the obtained results with the values tabulated in the literature: pumping speed measurements, leak measurements, outgassing

J. M. F. dos Santos

2005-01-01

64

A Solenoidal Spectrometer for the Undergraduate Laboratory  

Microsoft Academic Search

A solenoidal spectrometer is described. This instrument has several features which make it especially adaptable to use in an undergraduate laboratory. One is that the coil geometry is such that the student may readily verify that the range of variation of the magnetic field on the principal axis is less than 14 percent. Secondly, the baffle geometry is of sufficient

Byron T. Wright

1952-01-01

65

Simple vacuum experiments for undergraduate student laboratories  

Microsoft Academic Search

An overview of typical simple experiments, intended for didactic laboratory vacuum classes for undergraduate students majoring in Physics, Physics Engineering and Material Sciences, is presented. For all the experiments, the students perform actual measurements of characteristics of different vacuum components or material properties and compare the obtained results with the values tabulated in the literature: pumping speed measurements, leak measurements,

J. M. F. dos Santos

2005-01-01

66

A Mechanical Resonance Apparatus for Undergraduate Laboratories.  

ERIC Educational Resources Information Center

Reports the use of a heavy duty hacksaw blade and a 1000 turn pick-up coil to form the basis of a mechanical oscillator for a laboratory exercise in mechanical resonance designed for either the elementary undergraduate course or in association with an upper level mechanics course. (LZ)

Jones, Christopher C.

1995-01-01

67

Laser Mode Structure Experiments for Undergraduate Laboratories.  

ERIC Educational Resources Information Center

|Experiments dealing with laser mode structure are presented which are suitable for an upper division undergraduate laboratory. The theory of cavity modes is summarized. The mode structure of the radiation from a helium-neon laser is measured by using a photodiode detector and spectrum analyzer to detect intermode beating. Off-axial modes can be…

Phillips, Richard A.; Gehrz, Robert D.

68

Digital Storage Oscilloscopes in the Undergraduate Laboratory  

ERIC Educational Resources Information Center

|Digital storage oscilloscopes (DSOs) are now easily available to undergraduate laboratories. In many cases, a DSO can replace a data-acquisition system. Seven such experiments/demonstrations are considered: (i) families of "I-V" characteristics of electronic devices (bipolar junction transistor), (ii) the "V-I" curve of a high-temperature…

Kraftmakher, Yaakov

2012-01-01

69

DOE Science Undergraduate Laboratory Internships at Oak Ridge National Laboratory  

NSDL National Science Digital Library

DOE Science Undergraduate Laboratory Internships at Oak Ridge National Laboratory-- Description: Opportunities to participate in research in a broad range of science and engineering activities related to basic sciences, energy, and the environment. Discipline(s): Computer Science; Earth, Environmental, and Marine Sciences; Engineering; Life Sciences; Mathematics; Physical Sciences Eligibility: U.S. Citizens and Legal Permanent Residents. Undergraduate Students Location(s): Oak Ridge National Laboratory (Oak Ridge, Tennessee) Duration: Summer Term 10 Weeks; Fall/Spring Term 16 weeks Frequency: Spring, Summer, and Fall How to apply: http://www.scied.science.doe.gov Deadline(s): http://www.scied.science.doe.gov/scied/erulf/dates.html

70

An Undergraduate Laboratory Experiment for Upper-Level Forensic Science, Biochemistry, or Molecular Biology Courses: Human DNA Amplification Using STR Single Locus Primers by Real-Time PCR with SYBR Green Detection  

ERIC Educational Resources Information Center

|In this laboratory experiment, real-time polymerase chain reaction (real-time PCR) was conducted using published human TPOX single-locus DNA primers for validation and various student-designed short tandem repeat (STR) primers for Combined DNA Index System (CODIS) loci. SYBR Green was used to detect the amplification of the expected amplicons.…

Elkins, Kelly M.; Kadunc, Raelynn E.

2012-01-01

71

Probing Changes in the Conformation of tRNA[superscript Phe]: An Integrated Biochemistry Laboratory Course  

ERIC Educational Resources Information Center

|We have designed a new guided-inquiry laboratory for an advanced biochemistry course. This integrated laboratory focuses on the biomolecule tRNA[superscript Phe] and combines elements of bioorganic and bioinorganic chemistry with biochemistry. Throughout the semester students work together to study tRNA[superscript Phe] structure and ligand…

Kirk, Sarah R.; Silverstein, Todd P.; McFarlane Holman, Karen L.; Taylor, Buck L. H.

2008-01-01

72

Teaching Writing Skills in the Undergraduate Laboratory  

NASA Astrophysics Data System (ADS)

Stanford University's Writing in the Major program (WIM) requires every undergraduate degree program to include a writing course specific to its field of study. In the physics department's WIM course, undergraduates learn writing skills by composing laboratory reports in the form of journal articles. While studying such topics as scintillation and population inversion, students also practice techniques for communicating the physics more effectively. Students learn how to select a thesis, organize a complex argument, write concisely, aim their content at the proper audience, prove their assertions, and revise a finished draft. Through clearer writing, students reach a clearer understanding of the physics, and the improvements in both understanding and communication stay with the students through later courses and into their graduate studies. Teaching assistants for the course also notice a marked improvement in their own writing skills.

Ugolini, Dennis W.

1998-04-01

73

A Pharmacy Practice Laboratory Exercise to Apply Biochemistry Concepts  

PubMed Central

Objectives To develop exercises that allow pharmacy students to apply foundational knowledge discussed in a first-professional year (P1) biochemistry course to specific disease states and patient scenarios. Design A pharmacy practice laboratory exercise was developed to accompany a lecture sequence pertaining to purine biosynthesis and degradation. The assignment required students to fill a prescription, provide patient counseling tips, and answer questions pertaining to the disease state, the underlying biochemical problem, and the prescribed medication. Assessment Students were graded on the accuracy with which they filled the prescription, provided patient counseling, and answered the questions provided. Overall, students displayed mastery in all of these areas. Additionally, students completed a course survey on which they rated this exercise favorably, noting that it helped them to integrate basic science concepts and pharmacy practice. Conclusion A laboratory exercise provided an opportunity for P1 students to apply foundational pharmacy knowledge to a patient case and can serve as a template for the design of additional exercises.

McFalls, Marsha A.

2010-01-01

74

The Arizona State University electrical engineering undergraduate open laboratory  

Microsoft Academic Search

This paper describes the undergraduate electrical engineering laboratories at Arizona State University, For the past few years, the laboratory exercises associated with 11 electrical engineering courses have been conducted in a single large room and, mostly, on an unscheduled basis. This open laboratory is available 74 h each week and contains the equipment necessary for most of the undergraduate electrical

J. C. Palais; C. G. Javurek

1996-01-01

75

Analysis of a p53 mutation associated with cancer susceptibility for biochemistry and genetic laboratory courses.  

PubMed

We have devised and implemented a module for an upper division undergraduate laboratory based on the amplification and analysis of a p53 polymorphism associated with cancer susceptibility. First, students collected a drop of peripheral blood cells using a sterile sting and then used FTA cards to extract the genomic DNA. The p53 region is then PCR amplified, and the PCR products are digested with the BstUI enzyme to detect the 72 codon polymorphism. Polyacrylamide gel electrophoresis is used to resolve the PCR products, and the results are statistically analyzed in the context of human population genetics. Blood samples in FTA cards were also collected from 50 women to detect the mutation in a wide range of ages and assess its relationship to familial cancer susceptibility. This module enables students to use materials and methods that are routinely used by scientific researchers to analyze polymorphisms. Therefore, it can be used for laboratory exercises in traditional biochemistry curricula as well as in the growing field of genomic science and education. PMID:21567743

Soto-Cruz, Isabel; Legorreta-Herrera, Martha

2009-07-01

76

Kinetics of Papain: An Introductory Biochemistry Laboratory Experiment  

NASA Astrophysics Data System (ADS)

Enzyme kinetics experiments are popular in the undergraduate laboratory. These experiments have pedagogic value because they reinforce the concepts of Michaelis-Menten kinetics covered in the lecture portion of the course and give students the experience of calculating kinetic constants from data they themselves have generated. In this experiment, we investigate the kinetics of the thiol protease papain. The source of the papain is commercially available papaya latex. A specific substrate, Na-benzoyl-arginine-p-nitroanilide (BAPNA), is used, which takes advantage of the fact that papain interacts with a phenylalanine residue two amino acids away from the peptide bond cleaved. Upon hydrolysis by papain, a bright yellow product is released, p-nitroaniline. This allows the reaction to be monitored spectrophotometrically by measuring the rate of formation of the p-nitroaniline product as a function of the increase in absorbance of the solution at the lmax of p-nitroaniline (400 nm) over time at various substrate concentrations. These data are used to plot a Lineweaver-Burk plot from which the vmax and KM are obtained. If time permits, students carry out additional investigations in which e of p-nitroaniline is measured, the enzyme solution protein concentration is measured, the enzyme purity is evaluated by SDS-PAGE, and a pH-rate profile is constructed from experimental data.

Cornely, Kathleen; Crespo, Eric; Earley, Michael; Kloter, Rachel; Levesque, Aime; Pickering, Mary

1999-05-01

77

A Multistep Synthesis for an Advanced Undergraduate Organic Chemistry Laboratory  

ERIC Educational Resources Information Center

Multistep syntheses are often important components of the undergraduate organic laboratory experience and a three-step synthesis of 5-(2-sulfhydrylethyl) salicylaldehyde was described. The experiment is useful as a special project for an advanced undergraduate organic chemistry laboratory course and offers opportunities for students to master a…

Chang Ji; Peters, Dennis G.

2006-01-01

78

A Multistep Synthesis for an Advanced Undergraduate Organic Chemistry Laboratory  

ERIC Educational Resources Information Center

|Multistep syntheses are often important components of the undergraduate organic laboratory experience and a three-step synthesis of 5-(2-sulfhydrylethyl) salicylaldehyde was described. The experiment is useful as a special project for an advanced undergraduate organic chemistry laboratory course and offers opportunities for students to master a…

Chang Ji; Peters, Dennis G.

2006-01-01

79

IWAR Range: A Laboratory for Undergraduate Information Assurance Education.  

National Technical Information Service (NTIS)

This paper describes a unique resource at West Point, the Information Analysis and Research Laboratory, referred to as the IWAR range. The IWAR range is an isolated laboratory used by undergraduate students and faculty researchers. The IWAR is a productio...

J. Schafer D. J. Ragsdale J. R. Surdu C. A. Carver

2000-01-01

80

Surface Light Scattering Adapted to the Advanced Undergraduate Laboratory  

Microsoft Academic Search

The adaptation of the surface laser light scattering research method to an advanced undergraduate physical chemistry, polymer chemistry, or materials science laboratory is described. The SLS method was made accessible to an undergraduate setting by replacing the spectrum analyzer or autocorrelator normally used for data acquisition with a relatively inexpensive digital oscilloscope. A comparison between data obtained with a digital

Danny G. Miles Jr.; Zhihao Yang; Hyuk Yu

2002-01-01

81

Kinetic Analysis of Metal Ions: An Undergraduate Laboratory Experiment.  

ERIC Educational Resources Information Center

Reports on the adaptation of a kinetic method of analysis of metal ions for use in an undergraduate teaching laboratory. Background information, procedures used, and analysis of typical results obtained are provided. (JN)

Williams, Kathryn R.

1985-01-01

82

Mass Spectrometry for Large Undergraduate Laboratory Sections  

NASA Astrophysics Data System (ADS)

Mass spectrometry is routinely covered in undergraduate organic chemistry courses and a number of valuable laboratory experiments featuring its use have been discussed (1-7). Although such experiments work well at institutions with limited laboratory enrollments, we typically teach laboratories with enrollments of 160 or more in which it is difficult to allow each student to carry out a meaningful "hands on" mass spectrometry experiment. Since we feel that some practical experience with this technique is important, we have designed a simple gas chromatography-mass spectrometry (gc/ms) exercise that allows each student to analyze the products of a simple synthesis that they have performed. The exercise starts with the microscale SN2 synthesis of 1-bromobutane from 1-butanol as described by Williamson (8). The students complete the synthesis and place one drop of the distilled product in a screw capped vial. The vials are then sealed, labeled with the students name and taken to the mass spectrometry laboratory by a teaching assistant. Students are instructed to sign up for a 20-min block of time over the next few days in order to analyze their sample. When the student arrives at the laboratory, he or she adds 1 ml CH2Cl2 to the sample and injects 0.3 microliters of the solution into the gas chromatograph. The samples typically contain the 1-butanol starting material and the 1-bromobutane product along with traces of dibutyl ether. The figure shows a mass chromatogram along with the mass spectra of the starting material and product from an actual student run. For this analysis to be applicable to large numbers of students, the gc separation must be as rapid as possible. We have been able to analyze each sample in 6 minutes on a 30 m DB-5 capillary column with the following temperature program: 70 oC for 1 min, 70-80 oC at 10 oC/min, 86-140 oC at 67.5 oC/min, 140-210 oC at 70 oC/min, and 210 oC for 1 min. A mass range of 20-200 amu is scanned with a solvent delay of 2 min. Under these conditions each analysis takes the student about 10 min and two students are scheduled per 20 min block. Since the instrument is under computer control, students operate the computer during the run. As the peaks appear on the mass chromatogram, their mass spectra are obtained and the student decides which corresponds to product and evaluates product purity and the structure of impurities. There is ample time to display all spectra, conduct library searches, and print data. This relatively simple laboratory exercise has the advantage of allowing each student to carry out an analysis on his or her own product. The fact that a brominated product is obtained introduces a discussion of isotopic patterns in mass spectrometry. The experiment is scheduled to coincide with the lecture discussion of spectroscopic structure determination and after SN2 reactions have been covered. One of our mass spectrometer satellite data stations is interfaced with a "3-gun" projector in a large lecture hall allowing the display of actual mass spectra from our instrument to lecture sections. AcknowlegementThis work was partially supported by a grant, DUE9350846, from the National Science Foundation Division of Undergraduate Education Instrumentation and Laboratory Improvement Program. Literature Cited Brush, R. C.; Rice, G. W. J. Chem. Educ. 1994, 71, A293-A294. Asleson, G. L.; Doig, M. T.; Heldrich, F. J. J. Chem. Educ. 1993, 70, A290. Novak, M.; Heinrich, J. J. Chem. Educ. 1993, 70, A150. (b) Novak, M.; Heinrich, J.; Martin, K. A. J. Chem. Educ. 1993, 70, A103-104. Harman, C. S.; Myers, D. P.; Rittle, K. J. J. Chem. Educ. 1991, 68, 438-42. Mabbott, G. A. J. Chem. Educ. 1990, 67, 441-5. Hill, D. W.; McSharry, B. T.; Trzupek. J. Chem. Educ. 1988, 65, 907-10. Williamson, K. L. Macroscale and Microscale Organic Experiments, 2nd ed.; D. C. Heath: Lexington, MA, 1994; pp 247-51.

Illies, A.; Shevlin, P. B.; Childers, G.; Peschke, M.; Tsai, J.

1995-08-01

83

Teaching Protein Purification and Characterization Techniques: A Student-Initiated, Project-Oriented Biochemistry Laboratory Course  

ERIC Educational Resources Information Center

|This report describes a biochemistry laboratory that is completely project-oriented. Upper-level biology and chemistry majors work in teams to purify a protein of their choice. After the student groups have completed literature searches, ordered reagents, and made buffers they continue to learn basic protein purification and biochemical…

MacDonald, Gina

2008-01-01

84

Use of Solid Phase Extraction in the Biochemistry Laboratory to Separate Different Lipids  

ERIC Educational Resources Information Center

|Solid-phase extraction (SPE) was used to demonstrate how various lipids and lipid classes could be separated in a biochemistry laboratory setting. Three different SPE methods were chosen on their ability to separate a lipid mixture, consisting of a combination of a either a fatty acid, a triacylglycerol, a mono- or diacylglycerol, phospholipid,…

Flurkey, William H.

2005-01-01

85

Computer graphics in the undergraduate biology laboratory  

Microsoft Academic Search

Computer graphics use by biology undergraduate students is now a mainstay in the microanatomy course taught at Trinity University. By using desktop computer graphics, these students are able to take ownership of histological images in ways not possible just five years ago. Digital frame grabs of microscope slides serve as the basis of student produced graphics. These graphics allow the

Robert V. Blystone

1994-01-01

86

Reaction Kinetics: An Experiment for Biochemistry and Organic Chemistry Laboratories.  

ERIC Educational Resources Information Center

Describes an experiment to examine the kinetics of carbamate decomposition and the effect of buffer catalysis on the reaction. Includes background information, laboratory procedures, evaluation of data, and teaching suggestions. (Author/JN)

Ewing, Sheila

1982-01-01

87

Information Management Systems in the Undergraduate Instrumental Analysis Laboratory.  

ERIC Educational Resources Information Center

|Discusses two applications of Laboratory Information Management Systems (LIMS) in the undergraduate laboratory. They are the coulometric titration of thiosulfate with electrogenerated triiodide ion and the atomic absorption determination of calcium using both analytical calibration curve and standard addition methods. (JN)|

Merrer, Robert J.

1985-01-01

88

A laboratory based capstone course in computer security for undergraduates  

Microsoft Academic Search

We describe a laboratory based capstone course in computer security for undergraduates. The course is based on a sequence of hands-on laboratory exercises for four teams of students. It emphasizes defensive tools and techniques at the expense of attacks; it also takes a network centered view where student teams set up and configure entire networks. In this paper, we describe

Mike O'Leary

2006-01-01

89

Measuring Stellar Temperatures: An Astrophysical Laboratory for Undergraduate Students  

ERIC Educational Resources Information Center

|While astrophysics is a fascinating subject, it hardly lends itself to laboratory experiences accessible to undergraduate students. In this paper, we describe a feasible astrophysical laboratory experience in which the students are guided to take several stellar spectra, using a telescope, a spectrograph and a CCD camera, and perform a full data…

Cenadelli, D.; Zeni, M.

2008-01-01

90

An Undergraduate Nanotechnology Engineering Laboratory Course on Atomic Force Microscopy  

ERIC Educational Resources Information Center

|The University of Waterloo, Waterloo, ON, Canada, is home to North America's first undergraduate program in nanotechnology. As part of the Nanotechnology Engineering degree program, a scanning probe microscopy (SPM)-based laboratory has been developed for students in their fourth year. The one-term laboratory course "Nanoprobing and Lithography"…

Russo, D.; Fagan, R. D.; Hesjedal, T.

2011-01-01

91

An Undergraduate Nanotechnology Engineering Laboratory Course on Atomic Force Microscopy  

ERIC Educational Resources Information Center

The University of Waterloo, Waterloo, ON, Canada, is home to North America's first undergraduate program in nanotechnology. As part of the Nanotechnology Engineering degree program, a scanning probe microscopy (SPM)-based laboratory has been developed for students in their fourth year. The one-term laboratory course "Nanoprobing and Lithography"…

Russo, D.; Fagan, R. D.; Hesjedal, T.

2011-01-01

92

What skills should students of undergraduate biochemistry and molecular biology programs have upon graduation?  

PubMed

Biochemistry and molecular biology (BMB) students should demonstrate proficiency in the foundational concepts of the discipline and possess the skills needed to practice as professionals. To ascertain the skills that should be required, groups of BMB educators met in several focused workshops to discuss the expectations with the ultimate goal of clearly articulating the skills required. The results of these discussions highlight the critical importance of experimental, mathematical, and interpersonal skills including collaboration, teamwork, safety, and ethics. The groups also found experimental design, data interpretation and analysiand the ability to communicate findings to diverse audience to be essential skills. To aid in the development of appropriate assessments these skills are grouped into three categories, 1) Process of Science, 2) Communication and Comprehension of Science, and 3) Community of Practice Aspects of Science. Finally, the groups worked to align these competencies with the best practices in both teaching and in skills assessment. © 2013 by The International Union of Biochemistry and Molecular Biology, 41(5):297-301, 2013. PMID:24019246

White, Harold B; Benore, Marilee A; Sumter, Takita F; Caldwell, Benjamin D; Bell, Ellis

2013-09-10

93

Purification and characterization of enzymes from yeast: an extended undergraduate laboratory sequence for large classes.  

PubMed

Providing a project-based experience in an undergraduate biochemistry laboratory class can be complex with large class sizes and limited resources. We have designed a 6-week curriculum during which students purify and characterize the enzymes invertase and phosphatase from bakers yeast. Purification is performed in two stages via ethanol precipitation and anion exchange chromatography, and students perform both direct and coupled enzyme assays. By completion of the experimental series, students are able to identify which enzymes they have purified and have obtained kinetic parameters for one. This experimental series requires minimal instructor preparation time, is cost effective, and works with multiple sections of large groups of students. Students participating in this sequence showed increases in conceptual understanding of biochemical concepts as measured through in-class assessments and anonymous surveys. PMID:23868379

Johanson, Kelly E; Watt, Terry J; McIntyre, Neil R; Thompson, Marleesa

94

Undergraduate Laboratory Module on Skin Diffusion  

ERIC Educational Resources Information Center

|To introduce students to an application of chemical engineering directly related to human health, we developed an experiment for the unit operations laboratory at Georgia Tech examining diffusion across cadaver skin in the context of transdermal drug delivery. In this laboratory module, students prepare mouse skin samples, set up diffusion cells…

Norman, James J.; Andrews, Samantha N.; Prausnitz, Mark R.

2011-01-01

95

Micro-Raman spectroscopy in the undergraduate research laboratory  

Microsoft Academic Search

Modern materials science requires processing and characterization techniques for microscopic structures. Molecular probes such as Raman spectroscopy are some of the most viable tools, particularly if they are supplemented by imaging to obtain spatially resolved compositional information of inhomogeneous or low volume samples. In order to introduce these techniques and materials science experiments into the advanced undergraduate laboratory, we have

R. Voor; L. Chow; A. Schulte

1994-01-01

96

Protein gel electrophoresis in the undergraduate physics laboratory  

Microsoft Academic Search

We describe an undergraduate laboratory experiment in protein gel electrophoresis that uses readily available apparatus and materials. The separation of a mixture of stained proteins by gel electrophoresis was videotaped. Position-time data for the proteins generated from analysis of digitized videotape images allowed for calculation of protein terminal velocities. The dependence of protein terminal velocity on molar mass was determined

Danny G. Miles; David W. Bushman; Zhong-Ying Chen

2005-01-01

97

Magnetic Braking Revisited: Activities for the Undergraduate Laboratory  

ERIC Educational Resources Information Center

|This paper revisits the demonstration of Lenz by dropping magnets down a non-magnetic tube. Recent publications are reviewed and ideas for undergraduate laboratory investigations are suggested. Finally, an example of matching theory to observation is presented. (Contains 4 tables, 5 figures and 3 footnotes.)|

Ireson, Gren; Twidle, John

2008-01-01

98

An Integrated Enzyme Kinetics Laboratory Sequence for Undergraduates.  

ERIC Educational Resources Information Center

|Describes a three-week sequence to take undergraduate students through the study of enzyme kinetics in an integrated manner that reinforces the basic concepts of initial velocity and the effects of varying operational parameters. Discusses laboratory sessions and the use of a microcomputer in instruction. (CW)|

Bucholtz, Michael L.

1988-01-01

99

The Development of An Undergraduate Laboratory for Control System Design  

Microsoft Academic Search

An undergraduate laboratory for control system design at Texas A&M University consisting of eight experimental stations, each equipped with a computer and control system analysis and design software, interface hardware and software, and several physical plants that will be used for control experiments is described. Among these plants are: 1) a toy called Capsela, 2) a carousel, 3) a coupled

Garng M. Huang; Li-Fang Fu; John Fleming

1993-01-01

100

Seeding the Physical and Analytical Laboratory Curriculum with Interdisciplinary Applications  

Microsoft Academic Search

For the past five years, the Department of Chemistry and Biochemistry at the University of Maryland at College Park has worked to modernize all facets of the undergraduate laboratory experience. Students in the first-year biochemistry laboratory now utilize modern techniques in biochemistry and molecular biology to isolate and characterize the bacterial enzyme alkaline phosphatase. Organic chemistry laboratories are now conducted

Janice Reutt-Robey; Neil Blough; Richard Rebbert

1999-01-01

101

Analysis of a p53 Mutation Associated with Cancer Susceptibility for Biochemistry and Genetic Laboratory Courses  

ERIC Educational Resources Information Center

|We have devised and implemented a module for an upper division undergraduate laboratory based on the amplification and analysis of a p53 polymorphism associated with cancer susceptibility. First, students collected a drop of peripheral blood cells using a sterile sting and then used FTA cards to extract the genomic DNA. The p53 region is then PCR…

Soto-Cruz, Isabel; Legorreta-Herrera, Martha

2009-01-01

102

Materials physics: A new contemporary undergraduate laboratory  

Microsoft Academic Search

We have developed a laboratory course focusing on the physics of materials. This course, taught in place of a ``conventional'' lecture-only solid state or condensed matter physics course, helps to prepare students for the technical work force and also serves as a solid, broad-based foundation for students bound for graduate school. In addition, the course illustrates the increasingly interdisciplinary nature

Herbert Jaeger; Michael J. Pechan; Daniel K. Lottis

1998-01-01

103

The Most Proficient Enzyme as the Central Theme in an Integrated, Research-based Biochemistry Laboratory Course  

ERIC Educational Resources Information Center

|The enzyme orotidine-5'-monophosphate decarboxylase is an attractive choice for the central theme of an integrated, research-based biochemistry laboratory course. A series of laboratory exercises common to most instructional laboratories, including enzyme assays, protein purification, enzymatic characterization, elementary kinetics, and…

Smiley, Jeffrey A.

2002-01-01

104

An EPR Experiment for the Undergraduate Physical Chemistry Laboratory  

Microsoft Academic Search

An experiment that illustrates the principles of electron paramagnetic resonance spectroscopy in the undergraduate physical chemistry laboratory is described. Students measure the value of g for DPPH and use it to determine the value of g for two inorganic complexes, Cu(acac)2 and VO(acac)2. The students use two instruments: an instructional device that illustrates the principles of EPR and a commercial

R. A. Butera; D. H. Waldeck

2000-01-01

105

An Undergraduate Laboratory Activity Demonstrating Bacteriophage Specificity†  

PubMed Central

Bacteriophage are among the most diverse and numerous microbes inhabiting our planet. Yet many laboratory activities fail to engage students in meaningful exploration of their diversity, unique characteristics, and abundance. In this curriculum activity students use a standard plaque assay to enumerate bacteriophage particles from a natural sample and use the scientific method to address questions about host specificity and diversity. A raw primary sewage sample is enriched for bacteriophage using hosts in the family Enterobacteriaceae. Students hypothesize about host specificity and use quantitative data (serial dilution and plaque assay) to test their hypotheses. Combined class data also help them answer questions about phage diversity. The exercise was field tested with a class of 47 students using pre- and posttests. For all learning outcomes posttest scores were higher than pretest scores at or below p = 0.01. Average individualized learning gain (G) was also calculated for each learning outcome. Students’ use of scientific language in reference to bacteriophage and host interaction significantly improved (p = 0.002; G = 0.50). Improved means of expression helped students construct better hypotheses on phage host specificity (G = 0.31, p = 0.01) and to explain the plaque assay method (G = 0.33, p = 0.002). At the end of the exercise students also demonstrated improved knowledge and understanding of phage specificity as related to phage therapy in humans (p < 0.001; G = 51).

Allen, Mary E.; Gyure, Ruth A.

2013-01-01

106

Experiences of Mentors Training Underrepresented Undergraduates in the Research Laboratory  

PubMed Central

Successfully recruiting students from underrepresented groups to pursue biomedical science research careers continues to be a challenge. Early exposure to scientific research is often cited as a powerful means to attract research scholars with the research mentor being critical in facilitating the development of an individual's science identity and career; however, most mentors in the biological sciences have had little formal training in working with research mentees. To better understand mentors’ experiences working with undergraduates in the laboratory, we conducted semistructured interviews with 15 research mentors at a public university in the Midwest. The interviewed mentors were part of a program designed to increase the number of American Indians pursuing biomedical/biobehavioral research careers and represented a broad array of perspectives, including equal representation of male and female mentors, mentors from underrepresented groups, mentors at different levels of their careers, and mentors from undergraduate and professional school departments. The mentors identified benefits and challenges in being an effective mentor. We also explored what the term underrepresented means to the mentors and discovered that most of the mentors had an incomplete understanding about how differences in culture could contribute to underrepresented students’ experience in the laboratory. Our interviews identify issues relevant to designing programs and courses focused on undergraduate student research.

Prunuske, Amy J.; Wilson, Janelle; Walls, Melissa; Clarke, Benjamin

2013-01-01

107

A response to BIO 2010: Transforming undergraduate education for future research biologists, from the perspective of the biochemistry and molecular biology major program at Kenyon College.  

PubMed

The National Research Council completed a major study of undergraduate biology education, BIO 2010-Transforming Undergraduate Education For Future Research Biologists (BIO 2010), funded by the Howard Hughes Medical Institute and the National Institutes of Health. The BIO 2010 report recommends that biology pedagogy should use an interdisciplinary approach incorporating a strong basis in mathematics and physical sciences. Many of the aims of BIO 2010 can be met by an interdisciplinary major program such as that of Biochemistry and Molecular Biology at Kenyon College. The Kenyon program effectively encourages students to connect biology with chemistry and mathematics and to develop a sound basis for research in the biological sciences. A continuing challenge is to balance the needs for depth of physical and mathematical understanding and breadth of diversity in biology. PMID:21706713

Slonczewski, Joan L; Marusak, Rosemary

2004-05-01

108

Protein gel electrophoresis in the undergraduate physics laboratory  

NASA Astrophysics Data System (ADS)

We describe an undergraduate laboratory experiment in protein gel electrophoresis that uses readily available apparatus and materials. The separation of a mixture of stained proteins by gel electrophoresis was videotaped. Position-time data for the proteins generated from analysis of digitized videotape images allowed for calculation of protein terminal velocities. The dependence of protein terminal velocity on molar mass was determined and found to agree with predictions made by current theory. We also introduce a model that draws on simple physical concepts to help students place the experimental results in context.

Miles, Danny G.; Bushman, David W.; Chen, Zhong-Ying

2005-12-01

109

63 FR 7756 - Physics Laboratory 1998 Summer Undergraduate Research Fellowships (SURF)Partnerships in Atomic...  

Federal Register 2010, 2011, 2012, 2013

...Physics and Materials Science and Engineering Laboratory (MSEL) 1998 Summer Undergraduate...SURFing the Materials Science and Engineering Laboratory'' will provide an opportunity...PL) and the Materials Science and Engineering Laboratory (MSEL) of the...

1998-02-17

110

Taking a new biomarker into routine use - A perspective from the routine clinical biochemistry laboratory  

PubMed Central

There is increasing pressure to provide cost-effective healthcare based on “best practice.” Consequently, new biomarkers are only likely to be introduced into routine clinical biochemistry departments if they are supported by a strong evidence base and if the results will improve patient management and outcome. This requires convincing evidence of the benefits of introducing the new test, ideally reflected in fewer hospital admissions, fewer additional investigations and/or fewer clinic visits. Carefully designed audit and cost-benefit studies in relevant patient groups must demonstrate that introducing the biomarker delivers an improved and more effective clinical pathway. From the laboratory perspective, pre-analytical requirements must be thoroughly investigated at an early stage. Good stability of the biomarker in relevant physiological matrices is essential to avoid the need for special processing. Absence of specific timing requirements for sampling and knowledge of the effect of medications that might be used to treat the patients in whom the biomarker will be measured is also highly desirable. Analytically, automation is essential in modern high-throughput clinical laboratories. Assays must therefore be robust, fulfilling standard requirements for linearity on dilution, precision and reproducibility, both within- and between-run. Provision of measurements by a limited number of specialized reference laboratories may be most appropriate, especially when a new biomarker is first introduced into routine practice.

Sturgeon, Catharine; Hill, Robert; Hortin, Glen L; Thompson, Douglas

2010-01-01

111

Materials science undergraduate course laboratory in which students perform original research  

Microsoft Academic Search

A materials science course laboratory, in which undergraduate students perform original experimental research studies of properties of materials which evidently have not been studied elsewhere, has been implemented at the University of California, Santa Barbara, in the College of Engineering. It is suggested that a materials science course laboratory is a uniquely well-suited place in an undergraduate curriculum for allowing

Frederick Milstein; Paul W. Smith

1975-01-01

112

TRANSFERRING BEST PRACTICE FROM UNDERGRADUATE PRACTICAL TEACHING TO SECONDARY SCHOOLS: THE DYNAMIC LABORATORY MANUAL  

Microsoft Academic Search

Pre-laboratory work has been shown to be an effective investment at undergraduate level in chemistry at the University of Bristol. A Dynamic Laboratory Manual (DLM) has been developed to allow undergraduate students to rehearse practicals using virtual experiments, video clips and a range of assessment support. The DLM has been shown to be highly successful in its goals. In this

Timothy G. Harrison; Dudley E. Shallcross; William J. Heslop; John R. Eastman; Anthony J. Baldwin

113

Oxygen Uptake by a Cobalt(II) Complex: An Undergraduate Experiment  

ERIC Educational Resources Information Center

|An experimental procedure is described for studying oxygen uptake by a transition metal. This procedure is designed for use with undergraduates and may be used in organic or biochemistry laboratories. Diagrams of the apparatus are included. (MR)|

Appleton, Trevor G.

1977-01-01

114

Measurement of Phosphorylated Extracellular Signal-Regulated Kinase 1 and 2 in an Undergraduate Teaching Laboratory with ALPHAscreen Technology  

NSDL National Science Digital Library

Teaching the practical aspects of signal transduction to large undergraduate classes can be challenging when there is only a finite time frame in which to engage in laboratory activities. This teaching resource describes the use of bead-based ALPHAscreen technology for a class of 300 second-year biochemistry students, exposing the next generation of researchers to cutting-edge technology. Although in this case phosphorylated extracellular signal–regulated kinase 1 and 2 were measured, this technology is applicable to the measurement of many different signaling components. This resource provides a practical guide for instructors and exemplifies how such traditionally high-throughput research technologies can be used as teaching tools.

Debbie L. Hay (University of Auckland; School of Biological Sciences; REV)

2009-03-17

115

Experiments at the Triangle Universities Nuclear Laboratory for the undergraduate physics curriculum  

SciTech Connect

Experiments are being developed at the Triangle Universities Nuclear Laboratory to offer advanced undergraduate physics students laboratory experiences in the atmosphere of a frontier accelerator facility. These experiments differ from projects done by Undergraduate Research Assistants in that they are designed specifically for integration into the undergraduate curriculum as part of a structured laboratory course. The immediate goal of the program is to develop four accelerator-based experiments for use in the undergraduate Advanced Laboratory course at Duke University. Two newly developed experiments, {ital Carbon-Carbon Mott Scattering} and {ital Lifetime Measurements of an Auger Emitter}, will be described. In addition, the logistics of conducting undergraduate laboratory course work in an active research facility will be discussed. {copyright} {ital 1999 American Institute of Physics.}

Howell, C.R. [Department of Physics, Duke University and the Triangle Universities Nuclear Laboratory, Durham, North Carolina 27708 (United States)

1999-06-01

116

Experiments at the Triangle Universities Nuclear Laboratory for the undergraduate physics curriculum  

SciTech Connect

Experiments are being developed at the Triangle Universities Nuclear Laboratory to offer advanced undergraduate physics students laboratory experiences in the atmosphere of a frontier accelerator facility. These experiments differ from projects done by Undergraduate Research Assistants in that they are designed specifically for integration into the undergraduate curriculum as part of a structured laboratory course. The immediate goal of the program is to develop four accelerator-based experiments for use in the undergraduate Advanced Laboratory course at Duke University. Two newly developed experiments, Carbon-Carbon Mott Scattering and Lifetime Measurements of an Auger Emitter, will be described. In addition, the logistics of conducting undergraduate laboratory course work in an active research facility will be discussed.

Howell, C. R. [Department of Physics, Duke University and the Triangle Universities Nuclear Laboratory, Durham, North Carolina 27708 (United States)

1999-06-10

117

Planning an Objective and Need Based Curriculum: The Logistics with Reference to the Undergraduate Medical Education in Biochemistry  

PubMed Central

Purpose: The medical education is recently being transformed into several domains in order to adapt to the need and the value based academics which is required for the quality doctors who serve the community. Presently, the biochemistry curricula for the graduate students of medicine have been questioned by as many experts, because of their multiple lacunae. In this review, we would like to highlight the scenario which is related to the existing biochemistry curricula for graduate medical students, which have been followed in several medical schools and universities and we also hope to share our ideas for implementing objective and pragmatic curricula. Evidence based research, wherein the articles which are related to innovative teaching-learning tools are collected and the pros and cons which are related to the different methods analyzed in biochemistry point of view. Conclusion: Rapid changes in the content of the curriculum may not be required, but a gradual introduction of the novel approach and the methods of teaching biochemistry can be adopted into the curriculum.

Ramasamy, Ramesh; Gopal, Niranjan; Srinivasan, A R; Murugaiyan, Sathish Babu

2013-01-01

118

Planning an objective and need based curriculum: the logistics with reference to the undergraduate medical education in biochemistry.  

PubMed

Purpose: The medical education is recently being transformed into several domains in order to adapt to the need and the value based academics which is required for the quality doctors who serve the community. Presently, the biochemistry curricula for the graduate students of medicine have been questioned by as many experts, because of their multiple lacunae. In this review, we would like to highlight the scenario which is related to the existing biochemistry curricula for graduate medical students, which have been followed in several medical schools and universities and we also hope to share our ideas for implementing objective and pragmatic curricula. Evidence based research, wherein the articles which are related to innovative teaching-learning tools are collected and the pros and cons which are related to the different methods analyzed in biochemistry point of view. Conclusion: Rapid changes in the content of the curriculum may not be required, but a gradual introduction of the novel approach and the methods of teaching biochemistry can be adopted into the curriculum. PMID:23634431

Ramasamy, Ramesh; Gopal, Niranjan; Srinivasan, A R; Murugaiyan, Sathish Babu

2012-12-29

119

Is the Undergraduate Research Experience (URE) Always Best? The Power of Choice in a Bifurcated Practical Stream for a Large Introductory Biochemistry Class  

ERIC Educational Resources Information Center

|Science undergraduate courses typically cater to a mixed-learner cohort, with a diversity of motivations and skills. This diversity introduces pressure for designers of the practical laboratory curriculum. Students who are struggling with the course need a series of tasks that begin simply, and transition to more conceptually difficult material.…

Rowland, Susan L.; Lawrie, Gwen A.; Behrendorff, James B. Y. H.; Gillam, Elizabeth M. J.

2012-01-01

120

Undergraduate laboratory demonstration of aspects of phase transitions using Curie temperature determination in amorphous ferromagnetic materials  

Microsoft Academic Search

Traditionally, Curie temperature measurements have been performed using a vibrating sample magnetometer, an expensive piece of equipment not readily available to all undergraduate physics laboratories. This article describes an alternative measurement technique requiring only equipment stocked in most undergraduate physics departments. The device was originally constructed for data collection as part of a graduate-level research program in radiation damage effects

David G. Fisher; William T. Franz

1995-01-01

121

The impact of collaborative groups versus individuals in undergraduate inquiry-based astronomy laboratory learning exercises  

Microsoft Academic Search

One of the long-standing general undergraduate education requirements common to many colleges and universities is a science course with a laboratory experience component. One of the objectives frequently included in the description of most of these courses is that a student will understand the nature and processes of scientific inquiry. However, recent research has shown that learners in traditional undergraduate

Kendra J. Sibbernsen

2010-01-01

122

Structural Studies of Phycobiliproteins from Spirulina: Combining Spectroscopy, Thermodynamics, and Molecular Modeling in an Undergraduate Biochemistry Experiment  

NASA Astrophysics Data System (ADS)

Molecular modeling provides a powerful mechanism for students to connect molecular-level structural changes with macroscopically observable properties. We describe an experiment that integrates spectroscopy, thermodynamics, and molecular modeling into a single activity examining structural changes in phycobiliproteins upon denaturation with urea. Phycobiliproteins contain a covalently attached chromophore, phycocyanobilin, which is constrained in a planar conformation by the folded protein. Upon denaturation of the protein, the chromophore undergoes a conformational change, leading to a significant alteration of the absorption spectrum. By measuring the absorbance at 625 nm as a function of urea concentration, the free energy of unfolding can be determined. Students determine the dihedral angles in the chromophore and map the contacts between protein and chromophore using Protein Explorer, a structure visualization program freely available on the Internet. The change in absorption wavelength can be related to the difference between the LUMO and HOMO energies, obtained using PC Spartan Pro, for the chromophore in the folded and unfolded phycobiliprotein. This experiment could be used in a physical chemistry class in a curriculum that integrates biochemistry throughout the course work as well as in a traditional biochemistry course.

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Taylor, Ann T. S.; Feller, Scott E.

2002-12-01

123

DNA Fingerprint Analysis of Three Short Tandem Repeat (STR) Loci for Biochemistry and Forensic Science Laboratory Courses  

ERIC Educational Resources Information Center

|We have devised and implemented a DNA fingerprinting module for an upper division undergraduate laboratory based on the amplification and analysis of three of the 13 short tandem repeat loci that are required by the Federal Bureau of Investigation Combined DNA Index System (FBI CODIS) data base. Students first collect human epithelial (cheek)…

McNamara-Schroeder, Kathleen; Olonan, Cheryl; Chu, Simon; Montoya, Maria C.; Alviri, Mahta; Ginty, Shannon; Love, John J.

2006-01-01

124

The Quartz-Crystal Microbalance in an Undergraduate Laboratory Experiment: I. Fundamentals and Instrumentation  

ERIC Educational Resources Information Center

|The fundamentals, as well as the instrumentation of the quartz-crystal microbalance (QCM) technique that is used in an undergraduate laboratory experiment are being described. The QCM response can be easily used to change the properties of any system.|

Tsionsky, Vladimir

2007-01-01

125

Determination of Rate Constants for Ouabain Inhibition of Adenosine Triphosphatase: An Undergraduate Biological Chemistry Laboratory Experiment  

ERIC Educational Resources Information Center

|Describes an undergraduate biological chemistry laboratory experiment which provides students with an example of pseudo-first-order kinetics with the cardiac glycoside inhibition of mammalism sodium and potassium transport. (SL)|

Sall, Eri; And Others

1978-01-01

126

A Green Starting Material for Electrophilic Aromatic Substitution for the Undergraduate Organic Laboratory  

ERIC Educational Resources Information Center

Electrophilic aromatic substitution (EAS) experiment is designed for the second-semester and undergraduate organic chemistry laboratory. In the EAS experiment, the principles of green chemistry are discussed and illustrated in conjunction with the presentation of electrophilic aromatic substitution.

Jones-Wilson, T. Michelle; Burtch, Elizabeth A.

2005-01-01

127

A Green Starting Material for Electrophilic Aromatic Substitution for the Undergraduate Organic Laboratory  

ERIC Educational Resources Information Center

|Electrophilic aromatic substitution (EAS) experiment is designed for the second-semester and undergraduate organic chemistry laboratory. In the EAS experiment, the principles of green chemistry are discussed and illustrated in conjunction with the presentation of electrophilic aromatic substitution.|

Jones-Wilson, T. Michelle; Burtch, Elizabeth A.

2005-01-01

128

Chemical Analysis of Soils: An Environmental Chemistry Laboratory for Undergraduate Science Majors.  

ERIC Educational Resources Information Center

|Describes a laboratory exercise for undergraduate science students in which they evaluate soil samples for various parameters related to suitability for crop production and capability for retention of contaminants. (Contains 18 references.) (WRM)|

Willey, Joan D.; Avery, G. Brooks, Jr.; Manock, John J.; Skrabal, Stephen A.; Stehman, Charles F.

1999-01-01

129

Cross-Disciplinary Thermoregulation and Sweat Analysis Laboratory Experiences for Undergraduate Chemistry and Exercise Science Students  

NSDL National Science Digital Library

This article describes a qualitative evaluation of Cross-Disciplinary health sciences undergraduate laboratory experiences in which concepts and students from two distinct disciplines (chemistry and exercise physiology) combined to study exercise thermoregulation and sweat analysis.

2011-06-01

130

4,5-Diphenyl-1-methylimidazole: An Undergraduate Laboratory Experiment.  

ERIC Educational Resources Information Center

Background information and procedures used are provided for the synthesis of 4,5-diphenyl-methylimidazole. This experiment on the chemistry of heterocycles is ideally suited for beginning undergraduate organic chemistry students. (JN)

Anastas, Paul T.; And Others

1985-01-01

131

Development of sensorial experiments and their implementation into undergraduate laboratories  

NASA Astrophysics Data System (ADS)

"Visualization" of chemical phenomena often has been limited in the teaching laboratories to the sense of sight. We have developed chemistry experiments that rely on senses other than eyesight to investigate chemical concepts, make quantitative determinations, and familiarize students with chemical techniques traditionally designed using only eyesight. Multi-sensory learning can benefit all students by actively engaging them in learning through stimulation or an alternative way of experiencing a concept or ideas. Perception of events or concepts usually depends on the information from the different sensory systems combined. The use of multi-sensory learning can take advantage of all the senses to reinforce learning as each sense builds toward a more complete experience of scientific data. Research has shown that multi-sensory representations of scientific phenomena is a valuable tool for enhancing understanding of chemistry as well as displacing misconceptions through experience. Multi-sensory experiences have also been shown to enrich memory performance. There are few experiments published which utilize multiple senses in the teaching laboratory. The sensorial experiments chosen were conceptually similar to experiments currently performed in undergraduate laboratories; however students collect different types of data using multi-sensory observations. The experiments themselves were developed by using chemicals that would provide different sensory changes or capitalizing on sensory observations that were typically overlooked or ignored and obtain similar and precise results as in traditional experiments. Minimizing hazards and using safe practices are especially essential in these experiments as students utilize senses traditionally not allowed to be used in the laboratories. These sensorial experiments utilize typical equipment found in the teaching laboratories as well as inexpensive chemicals in order to aid implementation. All experiments are rigorously tested for accuracy and all chemicals examined for safety prior to implementation. The pedagogical objectives were established of to provide the ability to develop and stimulate students' conceptual understanding. The educational assessments of these experiments are are fashioned using the framework chosen (Marzano and Kendall). All the experiments are designed as collaborative, inquiry-based experiments in aims of enhancing the students understanding of the subject and promote critical thinking skills. These experiments use an investigative approach rather than verification methods. Terminology and misconceptions of the experiment were evaluated to prevent misunderstanding or confusion during the experiment. Interventions to address these misconceptions and learning problems associated with the experiment were developed. We have developed the Learning Lab Report, LLR, as an alternative model for the traditional laboratory reports, with the goal of transforming the traditional reports into something more useful for both students and instructors. The educational strategies are employed to develop this format in order to promote students to think critically about the concepts and take an active involvement in learning. From the results of the LLR, all experiments were reviewed and re-written to address any learning problems. The sensorial experiments study several topics usually covered in the first 2 years of the chemistry curriculum (general and organic chemistry courses). The experiments implemented, organic qualitative analysis, esterification kinetics, Le Chatelier equilibrium, thermometric titrations and ASA kinetics, worked effectively as students were able to draw correct conclusions about the concepts from the data obtained. An olfactory titration using the smell of the rutabaga vegetable has been developed and thoroughly tested. The LLR was utilized with the equilibrium, titration and acetyl salicylic acid experiments. The details of the development, implementation of these sensorial experiments and the LLR and student results are discussed.

Bromfield Lee, Deborah Christina

132

The Advanced Undergraduate Nuclear and Atomic Laboratory at the University of North Texas  

Microsoft Academic Search

The UNT Department of Physics periodically offers an upper level elective undergraduate laboratory course covering a variety of topics related to nuclear and atomic physics. Topics range from gamma, x-ray, and alpha-particle spectroscopy to Rutherford scattering and nuclear reactions. A lecture component complements the laboratory exercises by addressing pertinent theoretical material. For the laboratory exercises, students utilize facilities in the

Duncan Weathers

2009-01-01

133

A case study of virtual circuit laboratory for undergraduate student courses  

Microsoft Academic Search

The purpose of this study is to design and develop a Web based circuit virtual laboratory for undergraduate student courses, which can be treated as an accessorial tool for enhancing instruction. The study firstly reviews the general concept of virtual laboratory and some related works. Secondly, the design and development of the system framework of this circuit virtual laboratory is

Gu Rong; Zhu Miaoliang; Dong Yabo; Shi Dandan; Wang Yonggu

2005-01-01

134

A Laboratory Course for Teaching Laboratory Techniques, Experimental Design, Statistical Analysis, and Peer Review Process to Undergraduate Science Students  

ERIC Educational Resources Information Center

|This article describes a 13-week laboratory course called Human Toxicology taught at the University of Otago, New Zealand. This course used a guided inquiry based laboratory coupled with formative assessment and collaborative learning to develop in undergraduate students the skills of problem solving/critical thinking, data interpretation and…

Gliddon, C. M.; Rosengren, R. J.

2012-01-01

135

An undergraduate computer engineering rapid systems prototyping design laboratory  

Microsoft Academic Search

This paper describes a new two-quarter undergraduate capstone design class in our computer engineering curriculum. Design groups comprised of students from several different areas of specialization (e.g., software systems, very large scale integration (VLSI) devices and circuits, and computer architecture) design, simulate, implement, and evaluate a complete computing system. Typical examples of projects in the current sequence include a pipelined

James O. Hamblen; Henry L. Owen; Sudhakar Yalamanchili; Binh Dao

1999-01-01

136

Coulometric Analysis Experiment for the Undergraduate Chemistry Laboratory  

ERIC Educational Resources Information Center

|An undergraduate experiment on coulometric analysis of four commercial household products is presented. A special type of coulometry cell made of polydimethylsiloxane (PDMS) polymer is utilized. The PDMS cell consists of multiple analyte compartments and an internal network of salt bridges. Experimental procedure for the analysis of the acid in a…

Dabke, Rajeev B.; Gebeyehu, Zewdu; Thor, Ryan

2011-01-01

137

Enhancing Undergraduate Agro-Ecological Laboratory Employment through Experiential Learning  

ERIC Educational Resources Information Center

|We piloted an educational model, the Sustainable Agriculture Scholars Program, linking research in organic agriculture to experiential learning activities for summer undergraduate employees in 2007 and 2008. Our objectives were to: (1) further student understanding of sustainable agriculture research, (2) increase student interest in sustainable…

Grossman, J. M.; Patel, M.; Drinkwater, L. E.

2010-01-01

138

A Survey of the Practices, Procedures, and Techniques in Undergraduate Organic Chemistry Teaching Laboratories  

ERIC Educational Resources Information Center

|A survey was conducted of four-year institutions that teach undergraduate organic chemistry laboratories in the United States. The data include results from over 130 schools, describes the current practices at these institutions, and discusses the statistical results such as the scale of the laboratories performed, the chemical techniques…

Martin, Christopher B.; Schmidt, Monica; Soniat, Michael

2011-01-01

139

Development of Hands-On CFD Educational Interface for Undergraduate Engineering Courses and Laboratories  

Microsoft Academic Search

Development described of an educational interface for hands-on student experience with computational fluid dynamics (CFD) for undergraduate engineering courses and laboratories. Project part of a three-year National Science Foundation sponsored Course, Curriculum and Laboratory Improvement - Educational Materials Development project with faculty partners from colleges of engineering at Iowa, Iowa State, Cornell and Howard universities along with industrial (commercial CFD

Fred Stern; Tao Xing; Don Yarbrough; Alric Rothmayer; Ganesh Rajagopalan; Prakash Otta; David Caughey; Rajesh Bhaskaran; Sonya Smith; Barbara Hutchings; Shane Moeykens

2004-01-01

140

Incorporation of plasma physics and plasma etching into the undergraduate microelectronics laboratory  

Microsoft Academic Search

Experiments encompassing basic plasma physics and etching have been incorporated into the undergraduate microelectronics laboratory at the University of New Mexico. This laboratory is part of a senior level lecture course designed to introduce electrical engineering students to the fundamentals of IC fabrication. A new sequence seeking understanding of the fundamental nature of plasmas used for materials processing is described,

Charles B. Fleddermann; John Montoya; Salvadore Guel; Stephan D. Hersee; Don Kendall; Kenneth Jungling

1993-01-01

141

Low-Temperature, High-Field Susceptometry and Magnetometry Experiments for Undergraduate Laboratories  

Microsoft Academic Search

Cryogenic susceptometer\\/magnetometers are instruments commonly used in research labs to study magnetic materials; however they can also be used in the undergraduate laboratory to teach fundamental physical principles such as quantized angular momentum, statistical mechanics, and exchange interactions. We have developed three solid-state physics experiments for our modern physics and advanced laboratory courses using a cryogenic susceptometer\\/magnetometer. Our \\

Charles Cunningham; Matthew Cochran; Adam Rinehart

1998-01-01

142

A Coastal Environment Field and Laboratory Activity for an Undergraduate Geomorphology Course  

ERIC Educational Resources Information Center

|A field and laboratory exercise for an undergraduate geomorphology class is described that focuses on the beach. The project requires one day of fieldwork and two laboratory sessions. In the field, students measure water surface fluctuations (waves) with a pressure sensor, survey beach profiles, collect sediment samples, and observe the beach…

Ellis, Jean T.; Rindfleisch, Paul R.

2006-01-01

143

Design of a Molecular Transport Laboratory for Undergraduate Biomedical Engineering Students  

Microsoft Academic Search

The project goal is to design a laboratory experience for undergraduate biomedical engineering students capable of demonstrating molecular transport mechanisms. The two transport methods of interest are diffusion and osmosis. To relate these methods to physiological processes, the proposed laboratory apparatus will be a model for both capillary filtration and cell membrane diffusion.

L. A. Steele; J. L. Cezeaux

2006-01-01

144

Conceptual Change in the Undergraduate Biology Teaching Laboratory: A "Type Specimen" Case Study.  

ERIC Educational Resources Information Center

|This paper introduces the biology laboratory as a learning project. Biology laboratories provide unique learning opportunities to students as traditional achievement outcome measures, and play an important role in the development of process skills, manual skills, and attitudes. However, there is insufficient data on undergraduate students…

Jones, Dorothy A.; Eichinger, David C.

145

A Microcomputer-Based Data Acquisition System for Use in Undergraduate Laboratories.  

ERIC Educational Resources Information Center

A laboratory computer system based on the Commodore PET 2001 is described including three applications for the undergraduate analytical chemistry laboratory: (1) recording a UV-visible absorption spectrum; (2) recording and use of calibration curves; and (3) recording potentiometric data. Lists of data acquisition programs described are available…

Johnson, Ray L.

1982-01-01

146

An Undergraduate Laboratory Experiment in Bioinorganic Chemistry: Ligation States of Myoglobin  

ERIC Educational Resources Information Center

|Although there are numerous inorganic model systems that are readily presented as undergraduate laboratory experiments in bioinorganic chemistry, there are few examples that explore the inorganic chemistry of actual biological molecules. We present a laboratory experiment using the oxygen-binding protein myoglobin that can be easily incorporated…

Bailey, James A.

2011-01-01

147

A Coastal Environment Field and Laboratory Activity for an Undergraduate Geomorphology Course  

ERIC Educational Resources Information Center

A field and laboratory exercise for an undergraduate geomorphology class is described that focuses on the beach. The project requires one day of fieldwork and two laboratory sessions. In the field, students measure water surface fluctuations (waves) with a pressure sensor, survey beach profiles, collect sediment samples, and observe the beach…

Ellis, Jean T.; Rindfleisch, Paul R.

2006-01-01

148

A Project-Based Biochemistry Laboratory Promoting the Understanding and Uses of Fluorescence Spectroscopy in the Study of Biomolecular Structures and Interactions  

ERIC Educational Resources Information Center

|A laboratory project for a first semester biochemistry course is described, which integrates the traditional classroom study of the structure and function of biomolecules with the laboratory study of these molecules using fluorescence spectroscopy. Students are assigned a specific question addressing the stability/function of lipids, proteins, or…

Briese, Nicholas; Jakubowsk, Henry V.

2007-01-01

149

Preparative Protein Production from Inclusion Bodies and Crystallization: A Seven-Week Biochemistry Sequence  

ERIC Educational Resources Information Center

|We describe how to produce and purify proteins from "Escherichia coli" inclusion bodies by adapting versatile, preparative-scale techniques to the undergraduate laboratory schedule. This 7-week sequence of experiments fits into an annual cycle of research activity in biochemistry courses. Recombinant proteins are expressed as inclusion bodies,…

Peterson, Megan J.; Snyder, W. Kalani; Westerman, Shelley; McFarland, Benjamin J.

2011-01-01

150

A Capstone Experimental Learning Exercise for Undergraduate Physiology Laboratory Courses  

NSDL National Science Digital Library

Description of a physiology laboratory course with a learning opportunity designed to extend the individual lessons performed in the laboratory course with self-directed learning through the contextual application of information in a clinical setting

PhD Carissa M. Krane (University of Dayton Department of Biology)

2005-12-01

151

Assessing practical laboratory skills in undergraduate molecular biology courses  

Microsoft Academic Search

This study explored a new strategy of assessing laboratory skills in a molecular biology course to improve: student effort in preparation for and participation in laboratory work; valid evaluation of learning outcomes; and students’ employment prospects through provision of evidence of their skills. Previously, assessment was based on written laboratory reports and examinations, not on the demonstration of practical skills

Lynne Hunt; Annette Koenders; Vidar Gynnild

2011-01-01

152

ATR-FTIR Spectroscopy in the Undergraduate Chemistry Laboratory: Part II--A Physical Chemistry Laboratory Experiment on Surface Adsorption  

ERIC Educational Resources Information Center

|Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy is a useful technique for measuring the infrared spectra of solids and liquids as well as probing adsorption on particle surfaces. The use of FTIR-ATR spectroscopy in organic and inorganic chemistry laboratory courses as well as in undergraduate research was presented…

Schuttlefield, Jennifer D.; Larsen, Sarah C.; Grassian, Vicki H.

2008-01-01

153

Practicing biology: Undergraduate laboratory research, persistence in science, and the impact of self-efficacy beliefs  

NASA Astrophysics Data System (ADS)

As undergraduate laboratory research internships become more popular and universities devote considerable resources towards promoting them, it is important to clarify what students specifically gain through involvement in these experiences and it is important to understand their impact on the science pipeline. By examining recent findings describing the primary benefits of undergraduate research participation, along with self-efficacy theory, this study aims to provide more explanatory power to the anecdotal and descriptive accounts regarding the relationship between undergraduate research experiences and interest in continuing in science. Furthermore, this study characterizes practices that foster students' confidence in doing scientific work with detailed description and analysis of the interactions of researchers in a laboratory. Phase 1 of the study, a survey of undergraduate biology majors (n=71) at a major research university, investigates the relationships among participation in biology laboratory research internships, biology laboratory self-efficacy strength, and interest in persisting in science. Phase 2 of the study, a two-year investigation of a university biology research laboratory, investigates how scientific communities of practice develop self-efficacy beliefs. The findings suggest that participation in lab internships results in increased interest in continuing in life science/biology graduate school and careers. They also suggest that a significant proportion of that interest is related to the students' biology laboratory self-efficacy. The findings of this study point to two primary ways that undergraduate research participation might work to raise self-efficacy strength. First, university research laboratory communities can provide students with a variety of resources that scaffold them into biology laboratory mastery experiences. Second, university research laboratory communities can provide students with coping and mastery Discourse models and mentors, offering them access to and practice with the social norms of an epistemic community. This study begins to integrate what is currently known regarding student motivation through research experience, traditionally studied by program evaluations, with social learning theories. Given the role strong self-efficacy beliefs play in motivating students, understanding the kinds of social interactions that develop science self-efficacy could provide important policy guidelines for primary investigators who work with undergraduates, department chairs, and other promoters of undergraduate research in colleges and universities.

Berkes, Elizabeth

154

Analysis of the Essential Nutrient Strontium in Marine Aquariums by Atomic Absorption Spectroscopy: An Undergraduate Analytical Chemistry Laboratory Exercise  

Microsoft Academic Search

An undergraduate atomic absorption spectroscopy (AAS) laboratory experiment is presented involving the analysis of the essential nutrient strontium in a real-life sample, sea water. The quantitative analysis of strontium in sea water is a problem well suited for an undergraduate analytical chemistry laboratory. Sea water contains numerous components which prevent the direct quantitative determination of strontium. Students learn first hand

Laurent D. Gilles de Pelichy; Carl Adam; Eugene T. Smith

1997-01-01

155

Validating the Collision-Dominated Child-Langmuir Law for a DC Discharge Cathode Sheath in an Undergraduate Laboratory  

ERIC Educational Resources Information Center

|In this paper, we propose a simple method of observing the collision-dominated Child-Langmuir law in the course of an undergraduate laboratory work devoted to studying the properties of gas discharges. To this end we employ the dc gas discharge whose properties are studied in sufficient detail. The undergraduate laboratory work itself is reduced…

Lisovskiy, V.; Yegorenkov, V.

2009-01-01

156

Assessing Practical Laboratory Skills in Undergraduate Molecular Biology Courses  

ERIC Educational Resources Information Center

|This study explored a new strategy of assessing laboratory skills in a molecular biology course to improve: student effort in preparation for and participation in laboratory work; valid evaluation of learning outcomes; and students' employment prospects through provision of evidence of their skills. Previously, assessment was based on written…

Hunt, Lynne; Koenders, Annette; Gynnild, Vidar

2012-01-01

157

Characterizing the Level of Inquiry in the Undergraduate Laboratory  

ERIC Educational Resources Information Center

Discrepancies abound in use of the word "inquiry." In this paper, the authors propose a quantitative rubric designed to characterize the level of inquiry in laboratory activities and laboratory curricula. They do not wish to answer the question, "What is inquiry?" but rather, provide a tool for identifying its varying degrees of student…

Buck, Laura B.; Bretz, Stacey Lowery; Towns, Marcy H.

2008-01-01

158

The software laboratory for undergraduate process control education  

Microsoft Academic Search

Simulation exercises are a preferred method for reinforcing process control principles. The Software Laboratory described in this paper enables the student to perform many simulation exercises, some via programming and some via interactive menus. The Software Laboratory facilitates student learning by using technology, terminology, and process examples that are consistent with a course textbook. All programs use the popular MATLAB

Thomas E. Marlin

1996-01-01

159

Undergraduate training in laboratory animal science: a challenge for change  

Microsoft Academic Search

LABORATORY animals are considered to be an 'obscure' specialist area by many veterinary surgeons, despite the fact that the knowledge they apply in their daily work and all of the medicines they use depend on research or testing using laboratory animals. The employment of veterinary surgeons in research is a well known aspect of the profession. Less well known is

JD Stewart

1995-01-01

160

Acoustic testing and modeling: an advanced undergraduate laboratory.  

PubMed

This paper describes an advanced laboratory course in acoustics, specifically targeted for students with an interest in engineering applications at a school with a strongly integrated industrial co-op program. The laboratory course is developed around a three-pronged approach to problem solving that combines and integrates theoretical models, computational models, and experimental data. The course is structured around modules that begin with fundamental concepts and build laboratory skills and expand the knowledge base toward a final project. Students keep a detailed laboratory notebook, write research papers in teams, and must pass laboratory certification exams. This paper describes the course layout and philosophy and shares personal experience from both faculty and student perspectives. PMID:22423802

Russell, Daniel A; Ludwigsen, Daniel O

2012-03-01

161

Creating a Research-Rich Chemistry Curriculum with an Integrated, Upper-Level-Undergraduate Laboratory Program  

ERIC Educational Resources Information Center

A new research-rich chemistry curriculum with an integrated, upper-level undergraduate laboratory program has recently been developed to impart better understanding to the students. The program is called Advanced techniques in Experimental Chemistry and helps prepare students for more real-world problems.

Gron, Liz U.; Hales, David A.; Teague, M. Warfield

2007-01-01

162

Testing plastic deformations of materials in the introductory undergraduate mechanics laboratory  

Microsoft Academic Search

Normally, a mechanics laboratory at the undergraduate level includes an experiment to verify compliance with Hooke's law in materials, such as a steel spring and an elastic rubber band. Stress–strain curves are found for these elements. Compression in elastic bands is practically impossible to achieve due to flaccidity. A typical experiment for the complete loading–unloading cycle is to subject a

C M Romo-Kröger

2012-01-01

163

Study of Polymer Glasses by Modulated Differential Scanning Calorimetry in the Undergraduate Physical Chemistry Laboratory  

Microsoft Academic Search

Recent technological advances in thermal analysis present educational opportunities. In particular, modulated differential scanning calorimetry (MDSC) can be used to contrast reversing and nonreversing processes in practical laboratory experiments. The introduction of these concepts elucidates the relationship between experimental timescales and reversibility. The latter is a key concept of undergraduate thermodynamics theory that deserves reinforcement. In this paper, the theory

J. C. W. Folmer; Stefan Franzen

2003-01-01

164

Measurement of thermal conductivity and heat capacity in an undergraduate physics laboratory  

Microsoft Academic Search

An undergraduate laboratory course experiment on thermal properties of low diffusivity materials is fully described. The experimental setup is of simple construction, not requiring the use of vacuum techniques nor temperature controllers. From the transient regime data, thermal conductivity as well as specific heat capacity can be determined.

J. H. Talpe; V. I. Bekeris; C. E. Acha

1990-01-01

165

Augmenting traditional undergraduate advanced laboratory experiments by automation using IEEE488 and RS232 interfaces  

Microsoft Academic Search

Meaningful and effective use of the computer in undergraduate physics teaching has been unexpectedly and somewhat paradoxically slow. Some upper division laboratory experiments are described in which the computer is used in a way that preserves the traditional value and importance of classic experiments and minimizes the intrusion of the computer. Experiments and the equipment are chosen to minimize programming

S. Nornes; Y. Z. Tu

1989-01-01

166

Permeability measurements in undergraduate vacuum laboratories: a simple experiment using a He leak detector  

Microsoft Academic Search

We describe a simple experiment intended for didactic laboratory vacuum classes of undergraduate courses, using a helium leak detector. The helium throughput flowing into the vacuum volume due to the permeability of materials can be taken as a real leak, which can be measured with the helium leak detector. The experiment allows students to perform actual measurements of helium permeability

J M F dos Santos; J F C A Veloso; C M B Monteiro

2004-01-01

167

Determination of rest mass energy of the electron—an undergraduate laboratory experiment  

Microsoft Academic Search

We present a simple Compton scattering experiment to determine the rest mass energy of the electron which is unique for graduate and undergraduate laboratories. In the present experiment, we have measured the energies of the backscattered gamma photons with an NaI(Tl) gamma ray spectrometer coupled to a 1 K multichannel analyser. In order to enhance the backscattered gamma photons, a

S B Hosur; N M Badiger

2007-01-01

168

Residual gas analysers in an undergraduate vacuum laboratory: a simple experiment involving direct quantitative measurements  

Microsoft Academic Search

We describe a simple experiment intended for didactic laboratory vacuum classes of undergraduate courses, using a residual gas analyser (RGA): the determination of the natural abundance of the different isotopes of a given element in a gas sample. In addition to the understanding of the RGA operation and handling principles, the experiment allows the students to perform direct quantitative measurements

J M F dos Santos; A M F Trindade; J F C A Veloso; C M B Monteiro

2004-01-01

169

Determination of rest mass energy of the electron---an undergraduate laboratory experiment  

Microsoft Academic Search

We present a simple Compton scattering experiment to determine the rest mass energy of the electron which is unique for graduate and undergraduate laboratories. In the present experiment, we have measured the energies of the backscattered gamma photons with an NaI(Tl) gamma ray spectrometer coupled to a 1 K multichannel analyser. In order to enhance the backscattered gamma photons, a

S. B. Hosur; N. M. Badiger

2007-01-01

170

Topics in Chemical Instrumentation: XCVIII. Experiments Involving Thermal Methods of Analysis for Undergraduate Chemistry Laboratories.  

ERIC Educational Resources Information Center

|Explains some experiments involving thermal methods of analysis for undergraduate chemistry laboratories. Some experiments are: (1) the determination of the density and degree of crystallinity of a polymer; and (2) the determination of the specific heat of a nonvolatile compound. (HM)|

Ewing, Galen W., Ed.

1978-01-01

171

Thermodynamics of Sodium Dodecyl Sulfate (SDS) Micellization: An Undergraduate Laboratory Experiment  

ERIC Educational Resources Information Center

|An undergraduate laboratory experiment is presented that allows a thermodynamic characterization of micelle formation of sodium dodecyl sulfate (SDS) in aqueous solutions. The critical micelle concentration (CMC) and the degree of micelle ionization (alpha) are obtained at different temperatures by conductimetry. The molar standard free energy…

Marcolongo, Juan P.; Mirenda, Martin

2011-01-01

172

Glucose Transport in Cultured Animal Cells: An Exercise for the Undergraduate Cell Biology Laboratory  

ERIC Educational Resources Information Center

|Membrane transport is a fundamental concept that undergraduate students of cell biology understand better with laboratory experience. Formal teaching exercises commonly used to illustrate this concept are unbiological, qualitative, or intricate and time consuming to prepare. We have developed an exercise that uses uptake of radiolabeled nutrient…

Ledbetter, Mary Lee S.; Lippert, Malcolm J.

2002-01-01

173

The Perceptions, Views and Opinions of University Students about Physics Learning during Undergraduate Laboratory Work  

ERIC Educational Resources Information Center

The physics laboratory has long been a distinctive feature of physics education. It has been given a central role in the teaching and learning of physics at school and undergraduate levels in universities. The literature indicates that science educators have suggested that there are academically rich benefits in the learning and understanding of…

Hanif, M.; Sneddon, P. H.; Al-Ahmadi, F. M.; Reid, N.

2009-01-01

174

The Perceptions, Views and Opinions of University Students about Physics Learning during Undergraduate Laboratory Work  

ERIC Educational Resources Information Center

|The physics laboratory has long been a distinctive feature of physics education. It has been given a central role in the teaching and learning of physics at school and undergraduate levels in universities. The literature indicates that science educators have suggested that there are academically rich benefits in the learning and understanding of…

Hanif, M.; Sneddon, P. H.; Al-Ahmadi, F. M.; Reid, N.

2009-01-01

175

SOFTICE: Facilitating both Adoption of Linux Undergraduate Operating Systems Laboratories and Students' Immersion in Kernel Code  

Microsoft Academic Search

This paper discusses how Linux clustering and virtual machine technologies can improve undergraduate students' hands-on experience in operating systems laboratories. Like similar projects, SOFTICE1 relies on User Mode Linux (UML) to provide students with privileged access to a Linux system without creating security breaches on the hosting network. We extend such approaches in two aspects. First, we propose to facilitate

Alessio Gaspar; Sarah Langevin; Joe Stanaback; Clark Godwin

176

Integrating computerized data acquisition and analysis into an undergraduate electric machines laboratory  

Microsoft Academic Search

The Ohio State University (OSU) Department of Electrical Engineering has maintained an electric machines laboratory in the undergraduate curriculum for many years. Safety has always been of paramount concern and therefore instrumentation and measurement procedures must be developed so as to allow safe operation of the equipment over a wide range of experimental tests Hall-effect voltage and current transducers have

D. G. Kastern

2000-01-01

177

Microfluidics in the Undergraduate Laboratory: Device Fabrication and an Experiment to Mimic Intravascular Gas Embolism  

ERIC Educational Resources Information Center

|A method to fabricate microfluidic devices and an experimental protocol to model intravascular gas embolism for undergraduate laboratories are presented. The fabrication process details how to produce masters on glass slides; these masters serve as molds to pattern channels in an elastomeric polymer that can be adhered to a substrate, resulting…

Jablonski, Erin L.; Vogel, Brandon M.; Cavanagh, Daniel P.; Beers, Kathryn L.

2010-01-01

178

Bacterial Production of Poly(3-hydroxybutyrate): An Undergraduate Student Laboratory Experiment  

ERIC Educational Resources Information Center

|As part of a multidisciplinary course that is cross-listed between five departments, we developed an undergraduate student laboratory experiment for culturing, isolating, and purifying the biopolymer, poly(3-hydroxybutyrate), PHB. This biopolyester accumulates in the cytoplasm of bacterial cells under specific growth conditions, and it has…

Burns, Kristi L.; Oldham, Charlie D.; May, Sheldon W.

2009-01-01

179

An Advanced Undergraduate Chemistry Laboratory Experiment Exploring NIR Spectroscopy and Chemometrics  

ERIC Educational Resources Information Center

|An advanced undergraduate chemistry laboratory experiment to study the advantages and hazards of the coupling of NIR spectroscopy and chemometrics is described. The combination is commonly used for analysis and process control of various ingredients used in agriculture, petroleum and food products.|

Wanke, Randall; Stauffer, Jennifer

2007-01-01

180

Computer Based Learning in an Undergraduate Physics Laboratory: Interfacing and Instrument Control Using Matlab  

ERIC Educational Resources Information Center

|In this paper we describe the recent changes to the curriculum of the second year practical laboratory course in the School of Physics and Astronomy at the University of Nottingham. In particular, we describe how Matlab has been implemented as a teaching tool and discuss both its pedagogical advantages and disadvantages in teaching undergraduate

Sharp, J. S.; Glover, P. M.; Moseley, W.

2007-01-01

181

The Quartz-Crystal Microbalance in an Undergraduate Laboratory Experiment: Measuring Mass  

ERIC Educational Resources Information Center

|The study explains the quartz-crystal microbalance (QCM) technique, which is often used as an undergraduate laboratory experiment for measuring the mass of a system. QCM can be used as a mass sensor only when the measured mass is rigidly attached to the surface.|

Tsionsky, Vladimir

2007-01-01

182

Topics in Chemical Instrumentation: XCVIII. Experiments Involving Thermal Methods of Analysis for Undergraduate Chemistry Laboratories.  

ERIC Educational Resources Information Center

Explains some experiments involving thermal methods of analysis for undergraduate chemistry laboratories. Some experiments are: (1) the determination of the density and degree of crystallinity of a polymer; and (2) the determination of the specific heat of a nonvolatile compound. (HM)

Ewing, Galen W., Ed.

1978-01-01

183

Testing Plastic Deformations of Materials in the Introductory Undergraduate Mechanics Laboratory  

ERIC Educational Resources Information Center

|Normally, a mechanics laboratory at the undergraduate level includes an experiment to verify compliance with Hooke's law in materials, such as a steel spring and an elastic rubber band. Stress-strain curves are found for these elements. Compression in elastic bands is practically impossible to achieve due to flaccidity. A typical experiment for…

Romo-Kroger, C. M.

2012-01-01

184

The Quartz-Crystal Microbalance in an Undergraduate Laboratory Experiment: Measuring Mass  

ERIC Educational Resources Information Center

The study explains the quartz-crystal microbalance (QCM) technique, which is often used as an undergraduate laboratory experiment for measuring the mass of a system. QCM can be used as a mass sensor only when the measured mass is rigidly attached to the surface.

Tsionsky, Vladimir

2007-01-01

185

Borohydride Reduction of Estrone: Demonstration of Diastereoselectivity in the Undergraduate Organic Chemistry Laboratory  

ERIC Educational Resources Information Center

This experiment presents a guided-inquiry approach to the demonstration of diastereoselectivity in an undergraduate organic chemistry laboratory. Chiral hindered ketones such as estrone, undergo facile reduction with sodium borohydride in a highly diastereoselective manner. The diastereomeric estradiols produced in the reaction can be analyzed and…

Aditya, Animesh; Nichols, David E.; Loudon, G. Marc

2008-01-01

186

Gas Clathrate Hydrates Experiment for High School Projects and Undergraduate Laboratories  

ERIC Educational Resources Information Center

|We present a laboratory procedure, suitable for high school and undergraduate students, for preparing and studying propane clathrate hydrate. Because of their gas storage potential and large natural deposits, gas clathrate hydrates may have economic importance both as an energy source and a transportation medium. Similar to pure ice, the gas…

Prado, Melissa P.; Pham, Annie; Ferazzi, Robert E.; Edwards, Kimberly; Janda, Kenneth C.

2007-01-01

187

Undergraduate Introductory Quantitative Chemistry Laboratory Course: Interdisciplinary Group Projects in Phytoremediation  

ERIC Educational Resources Information Center

|The laboratory course around the phytoremediation is designed to develop both individual skills and promote cooperative learning while starting students work on projects in a specific area of environmental chemistry and analysis. Many research-active undergraduate institutions have developed courses, which are interdisciplinary in nature that…

Van Engelen, Debra L.; Suljak, Steven W.; Hall, J. Patrick; Holmes, Bert E.

2007-01-01

188

A Green Polymerization of Aspartic Acid for the Undergraduate Organic Laboratory  

ERIC Educational Resources Information Center

The green polymerization of aspartic acid carried out during an organic-inorganic synthesis laboratory course for undergraduate students is described. The procedure is based on work by Donlar Corporation, a Peru, Illinois-based company that won a Green Chemistry Challenge Award in 1996 in the Small Business category for preparing thermal…

Bennett, George D.

2005-01-01

189

Cross-Disciplinary Thermoregulation and Sweat Analysis Laboratory Experiences for Undergraduate Chemistry and Exercise Science Students  

ERIC Educational Resources Information Center

|Cross-disciplinary (CD) learning experiences benefit student understanding of concepts and curriculum by offering opportunities to explore topics from the perspectives of alternate fields of study. This report involves a qualitative evaluation of CD health sciences undergraduate laboratory experiences in which concepts and students from two…

Mulligan, Gregory; Taylor, Nichole; Glen, Mary; Tomlin, Dona; Gaul, Catherine A.

2011-01-01

190

Simple & Rapid Generation of Complex DNA Profiles for the Undergraduate Laboratory  

ERIC Educational Resources Information Center

|Deoxyribonucleic acid (DNA) profiles can be generated by a variety of techniques incorporating different types of DNA markers. Simple methods are commonly utilized in the undergraduate laboratory, but with certain drawbacks. In this article, the author presents an advancement of the "Alu" dimorphism technique involving two tetraplex polymerase…

Kass, David H.

2007-01-01

191

Microfluidic Gel Electrophoresis in the Undergraduate Laboratory Applied to Food Analysis  

ERIC Educational Resources Information Center

|A microfluidics-based laboratory experiment for the analysis of DNA fragments in an analytical undergraduate course is presented. The experiment is set within the context of food species identification via amplified DNA fragments. The students are provided with berry samples from which they extract DNA and perform polymerase chain reaction (PCR)…

Chao, Tzu-Chiao; Bhattacharya, Sanchari; Ros, Alexandra

2012-01-01

192

Gas Clathrate Hydrates Experiment for High School Projects and Undergraduate Laboratories  

ERIC Educational Resources Information Center

We present a laboratory procedure, suitable for high school and undergraduate students, for preparing and studying propane clathrate hydrate. Because of their gas storage potential and large natural deposits, gas clathrate hydrates may have economic importance both as an energy source and a transportation medium. Similar to pure ice, the gas…

Prado, Melissa P.; Pham, Annie; Ferazzi, Robert E.; Edwards, Kimberly; Janda, Kenneth C.

2007-01-01

193

ATR-FTIR Spectroscopy in the Undergraduate Chemistry Laboratory: Part I--Fundamentals and Examples  

ERIC Educational Resources Information Center

|Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy is a useful technique for measuring the infrared spectra of solids and liquids as well as probing adsorption on particle surfaces. Several examples of the use of FTIR-ATR spectroscopy in different undergraduate chemistry laboratory courses are presented here. These…

Schuttlefield, Jennifer D.; Grassian, Vicki H.

2008-01-01

194

Borohydride Reduction of Estrone: Demonstration of Diastereoselectivity in the Undergraduate Organic Chemistry Laboratory  

ERIC Educational Resources Information Center

|This experiment presents a guided-inquiry approach to the demonstration of diastereoselectivity in an undergraduate organic chemistry laboratory. Chiral hindered ketones such as estrone, undergo facile reduction with sodium borohydride in a highly diastereoselective manner. The diastereomeric estradiols produced in the reaction can be analyzed…

Aditya, Animesh; Nichols, David E.; Loudon, G. Marc

2008-01-01

195

Examination of a Reaction Mechanism by Polarimetry: An Experiment for the Undergraduate Organic Chemistry Laboratory  

Microsoft Academic Search

An undergraduate Organic chemistry laboratory experiment involving the use of polarimetry to determine a reaction mechanism is described. The experiment has been shown to be successful with chiral mandelic acid and POCl3 and with chiral lactic acid and HBr. The substitution mechanisms of these reactions proceed with 1% and 38% SN2 character respectively.

Michael D. Mosher; Chad O. Kelly; Melvyn W. Mosher

1996-01-01

196

Advanced Undergraduate-Laboratory Experiment on Electron Spin Resonance in Single-Crystal Ruby  

ERIC Educational Resources Information Center

An electron-spin-resonance experiment which has been successfully performed in an advanced undergraduate physics laboratory is described. A discussion of that part of the theory of magnetic resonance necessary for the understanding of the experiment is also provided in this article. (DT)

Collins, Lee A.; And Others

1974-01-01

197

Interactive online undergraduate laboratories using J-DSP  

Microsoft Academic Search

An interactive Web-based simulation tool called Java-DSP (J-DSP) for use in digital signal processing (DSP)-related electrical engineering courses is described. J-DSP is an object-oriented simulation environment that enables students and distance learners to perform online signal processing simulations, visualize Web-based interactive demos, and perform computer laboratories from remote locations. J-DSP is accompanied by a series of hands-on laboratory exercises that

Andreas Spanias; Venkatraman Atti

2005-01-01

198

Observing thermomigration of air bubbles in a fluid under gravity at an undergraduate research laboratory  

NASA Astrophysics Data System (ADS)

An educational experimental project in fluid mechanics for space science or physics honors undergraduates is presented. The motion of air bubbles in a viscous silicone oil in response to buoyancy and Marangoni convection is studied with an experimental setup adapted to the undergraduate laboratory. The Marangoni effect or thermocapillary convection occurs when a temperature gradient is present in the oil which generates a surface tension differential over the bubbles. Results of measured radii and temperature gradients necessary to balance the buoyancy and Marangoni forces, along with computed Marangoni velocities are in good agreement with previous terrestrial and space experiments.

Simard, J.-F.; Houdayer, A.; Boudreault, R.; Bélanger, S.; Laaouan, A.

1995-09-01

199

Adsorption of Phosphate on Goethite: An Undergraduate Research Laboratory Project  

ERIC Educational Resources Information Center

|A laboratory experiment on the adsorption of phosphate on goethite is presented, which also includes discussion on surface properties, interfaces, acid-base equilibrium, molecular structure and solid state chemistry. It was seen that many students were able to produce qualitatively correct results for a complex system of real interest and they…

Tribe, Lorena; Barja, Beatriz C.

2004-01-01

200

High-resolution solar spectroscopy in the undergraduate physics laboratory  

Microsoft Academic Search

The richness of the solar spectrum at visible wavelengths makes it ideally suited for many laboratory exercises in optical spectroscopy. A number of such experiments taking advantage of a high-resolution scanning spectrometer are described as they have been performed by seniors at Middlebury College. Physical principles emphasized include optical depth, the nature of molecular spectra, the Doppler effect, and the

Stephen J. Ratcliff; Darcy K. Noss; Jeffrey S. Dunham; Eric B. Anthony; John H. Cooley; Alberto Alvarez

1992-01-01

201

Development of Sensorial Experiments and Their Implementation into Undergraduate Laboratories  

ERIC Educational Resources Information Center

|"Visualization" of chemical phenomena often has been limited in the teaching laboratories to the sense of sight. We have developed chemistry experiments that rely on senses other than eyesight to investigate chemical concepts, make quantitative determinations, and familiarize students with chemical techniques traditionally designed using only…

Bromfield Lee, Deborah Christina

2009-01-01

202

Glycosidation of Methanol with Ribose: An Interdisciplinary Undergraduate Laboratory Experiment  

ERIC Educational Resources Information Center

This exercise provides students hands-on experience with the topics of glycosidation, hemiacetal and acetal formation, proton nuclear magnetic resonance ([superscript 1]H NMR) spectroscopy, and kinetic and thermodynamic product formation. In this laboratory experiment, the methyl acetal of ribose is synthesized, and the kinetic and thermodynamic…

Simon, Erin; Cook, Katie; Pritchard, Meredith R.; Stripe, Wayne; Bruch, Martha; Bendinskas, Kestutis

2010-01-01

203

Creatine Synthesis: An Undergraduate Organic Chemistry Laboratory Experiment  

ERIC Educational Resources Information Center

Students in introductory chemistry classes typically appreciate seeing the connection between course content and the "real world". For this reason, we have developed a synthesis of creatine monohydrate--a popular supplement used in sports requiring short bursts of energy--for introductory organic chemistry laboratory courses. Creatine monohydrate…

Smith, Andri L.; Tan, Paula

2006-01-01

204

A Green Enantioselective Aldol Condensation for the Undergraduate Organic Laboratory  

ERIC Educational Resources Information Center

|A number of laboratory exercises for the organic chemistry curriculum that emphasize enantioselective synthesis of the aldol condensation which involves the proline-catalyzed condensation between acetone and isobutyraldehyde are explored. The experiment illustrates some of the trade-offs involved in green chemistry like the use of acetone in…

Bennett, George D.

2006-01-01

205

Vectors and Fomites: An Investigative Laboratory for Undergraduates.  

ERIC Educational Resources Information Center

|Presents a laboratory model system for introductory microbiology students that involves hands-on studies of bacteria vectored in soil nematodes. Describes a series of experiments designed to demonstrate vector-fomite transmission, bacterial survival, and disinfectant activity. Introduces the concept of genetically engineered microorganisms and…

Adamo, Joseph A.; Gealt, Michael A.

1996-01-01

206

A Green Enantioselective Aldol Condensation for the Undergraduate Organic Laboratory  

ERIC Educational Resources Information Center

A number of laboratory exercises for the organic chemistry curriculum that emphasize enantioselective synthesis of the aldol condensation which involves the proline-catalyzed condensation between acetone and isobutyraldehyde are explored. The experiment illustrates some of the trade-offs involved in green chemistry like the use of acetone in large…

Bennett, George D.

2006-01-01

207

An Enzyme Kinetics Experiment for the Undergraduate Organic Chemistry Laboratory  

ERIC Educational Resources Information Center

An experiment using [superscript 1]H NMR spectroscopy to observe the kinetics of the acylase 1-catalyzed hydrolysis of "N"-acetyl-DL-methionine has been developed for the organic laboratory. The L-enantiomer of the reactant is hydrolyzed completely in less than 2 h, and [superscript 1]H NMR spectroscopic data from a single sample can be worked up…

Olsen, Robert J.; Olsen, Julie A.; Giles, Greta A.

2010-01-01

208

Adsorption of Phosphate on Goethite: An Undergraduate Research Laboratory Project  

ERIC Educational Resources Information Center

A laboratory experiment on the adsorption of phosphate on goethite is presented, which also includes discussion on surface properties, interfaces, acid-base equilibrium, molecular structure and solid state chemistry. It was seen that many students were able to produce qualitatively correct results for a complex system of real interest and they…

Tribe, Lorena; Barja, Beatriz C.

2004-01-01

209

An Enzyme Kinetics Experiment for the Undergraduate Organic Chemistry Laboratory  

ERIC Educational Resources Information Center

|An experiment using [superscript 1]H NMR spectroscopy to observe the kinetics of the acylase 1-catalyzed hydrolysis of "N"-acetyl-DL-methionine has been developed for the organic laboratory. The L-enantiomer of the reactant is hydrolyzed completely in less than 2 h, and [superscript 1]H NMR spectroscopic data from a single sample can be worked up…

Olsen, Robert J.; Olsen, Julie A.; Giles, Greta A.

2010-01-01

210

Creatine Synthesis: An Undergraduate Organic Chemistry Laboratory Experiment  

ERIC Educational Resources Information Center

|Students in introductory chemistry classes typically appreciate seeing the connection between course content and the "real world". For this reason, we have developed a synthesis of creatine monohydrate--a popular supplement used in sports requiring short bursts of energy--for introductory organic chemistry laboratory courses. Creatine monohydrate…

Smith, Andri L.; Tan, Paula

2006-01-01

211

Glycosidation of Methanol with Ribose: An Interdisciplinary Undergraduate Laboratory Experiment  

ERIC Educational Resources Information Center

|This exercise provides students hands-on experience with the topics of glycosidation, hemiacetal and acetal formation, proton nuclear magnetic resonance ([superscript 1]H NMR) spectroscopy, and kinetic and thermodynamic product formation. In this laboratory experiment, the methyl acetal of ribose is synthesized, and the kinetic and thermodynamic…

Simon, Erin; Cook, Katie; Pritchard, Meredith R.; Stripe, Wayne; Bruch, Martha; Bendinskas, Kestutis

2010-01-01

212

What Are Undergraduates Doing at Biological Field Stations and Marine Laboratories?  

NSDL National Science Digital Library

Biological field stations and marine laboratories (FSMLs) serve as places to study the natural environment in a variety of ways, from the level of the molecule to the globe. Undergraduate opportunities at FSMLs reflect the diversity of study optionsâÂÂformal courses, research and service internships, and field-trip experiencesâÂÂand students are responding to those opportunities: More than half of the FSMLs that responded to an informal survey indicated an increase in their undergraduate enrollment in the past 10 years. Many programs are residential in nature, which facilitates the development of a community of scholars in which undergraduates can interact not just with their peers but also with graduate students, research assistants, postdoctoral fellows, and resident and visiting faculty. With respect to undergraduates, challenges for FSMLs include maintaining relevance in curricular offerings, attracting rigorous and well-trained instructors, providing adequate numbers of mentors for research experiences, and providing funding to assist undergraduates who want to study at a FSML.

Janet Hodder (University of Oregon;Institute of Marine Biology)

2009-09-01

213

Introducing Undergraduate Students to Real-Time PCR  

ERIC Educational Resources Information Center

|An experiment is conducted, which in four 3 h laboratory sessions, introduces third year undergraduate Biochemistry students to the technique of real-time PCR in a biological context. The model used is a murine erythroleukemia cell line (MEL cells). These continuously cycling, immature red blood cells, arrested at an early stage in…

Hancock, Dale; Funnell, Alister; Jack, Briony; Johnston, Jill

2010-01-01

214

Bean Beetles A Model Organism for Undergraduate Laboratories  

NSDL National Science Digital Library

Each classroom-tested experiment (in the Laboratory Activities part of the website), has a student handout, instructor notes, and sample data. All these materials may be viewed on the website and are downloadable (MS word and pdf formats). Additional downloadable materials include graphs of sample data (PowerPoint slides), and images for identifying the sexes and recognizing eggs on a bean (PowerPoint slides).

Lawrence S. Blumer (Morehouse College;); Christopher W. Beck (Emory University;)

2005-05-31

215

The impact of collaborative groups versus individuals in undergraduate inquiry-based astronomy laboratory learning exercises  

NASA Astrophysics Data System (ADS)

One of the long-standing general undergraduate education requirements common to many colleges and universities is a science course with a laboratory experience component. One of the objectives frequently included in the description of most of these courses is that a student will understand the nature and processes of scientific inquiry. However, recent research has shown that learners in traditional undergraduate science laboratory environments are not developing a sufficiently meaningful understanding of scientific inquiry. Recently, astronomy laboratory activities have been developed that intentionally scaffold a student from guided activities to open inquiry ones and preliminary results show that these laboratories are successful for supporting students to understand the nature of scientific inquiry (Slater, S., Slater, T. F., & Shaner, 2008). This mixed-method quasi-experimental study was designed to determine how students in an undergraduate astronomy laboratory increase their understanding of inquiry working in relative isolation compared to working in small collaborative learning groups. The introductory astronomy laboratory students in the study generally increased their understanding of scientific inquiry over the course of the semester and this held true similarly for students working in groups and students working individually in the laboratories. This was determined by the examining the change in responses from the pretest to the posttest administration of the Views of Scientific Inquiry (VOSI) survey, the increase in scores on laboratory exercises, and observations from the instructor. Because the study was successful in determining that individuals in the astronomy laboratory do as well at understanding inquiry as those who complete their exercises in small groups, it would be appropriate to offer these inquiry-based exercises in an online format.

Sibbernsen, Kendra J.

216

Green, Enzymatic Syntheses of Divanillin and Diapocynin for the Organic, Biochemistry, or Advanced General Chemistry Laboratory  

ERIC Educational Resources Information Center

Environmentally benign chemistry is an increasingly important topic both in the classroom and the laboratory. In this experiment, students synthesize divanillin from vanillin or diapocynin from apocynin, using horseradish peroxidase and hydrogen peroxide in water. The dimerized products form rapidly at ambient temperature and are isolated by…

Nishimura, Rachel T.; Giammanco, Chiara H.; Vosburg, David A.

2010-01-01

217

Green, Enzymatic Syntheses of Divanillin and Diapocynin for the Organic, Biochemistry, or Advanced General Chemistry Laboratory  

ERIC Educational Resources Information Center

|Environmentally benign chemistry is an increasingly important topic both in the classroom and the laboratory. In this experiment, students synthesize divanillin from vanillin or diapocynin from apocynin, using horseradish peroxidase and hydrogen peroxide in water. The dimerized products form rapidly at ambient temperature and are isolated by…

Nishimura, Rachel T.; Giammanco, Chiara H.; Vosburg, David A.

2010-01-01

218

AFHRL/FT [Air Force Human Resources Laboratory/Flight Training] Capabilities in Undergraduate Pilot Training Simulation Research: Executive Summary.  

ERIC Educational Resources Information Center

|The document presents a summary description of the Air Force Human Resource Laboratory's Flying Training Division (AFHRL/FT) research capabilities for undergraduate pilot training. One of the research devices investigated is the Advanced Simulator for Undergraduate Pilot Training (ASUPT). The equipment includes the ASUPT, the instrumented T-37…

Matheny, W. G.; And Others

219

Synthesis of a Partially Protected Azidodeoxy Sugar. A Project Suitable for the Advanced Undergraduate Organic Chemistry Laboratory  

Microsoft Academic Search

The synthetic chemistry of carbohydrates provides a wealth of possible experiments for the undergraduate organic chemistry laboratory. However, few appropriate examples have been developed to date. With this simple two-step synthesis of a partially protected azidodeoxy sugar, we demonstrate several important concepts introduced in undergraduate chemistry (alcohol activation, steric hindrance, nucleophilic substitution) while offering products that are readily amenable to

Peter Norris; Scott Freeze; Christopher J. Gabriel

2001-01-01

220

Genesis of "Biochemistry: A Problems Approach"  

ERIC Educational Resources Information Center

When the author began teaching as a young assistant professor at Caltech in 1966, his assignment was to take over the undergraduate biochemistry course taught for many years by Henry Borsook, who was about to retire. Students dreaded this course. Having delighted in biochemistry during his graduate training at Stanford, he was determined to put…

Wood, William B.

2002-01-01

221

Genesis of "Biochemistry: A Problems Approach"  

ERIC Educational Resources Information Center

|When the author began teaching as a young assistant professor at Caltech in 1966, his assignment was to take over the undergraduate biochemistry course taught for many years by Henry Borsook, who was about to retire. Students dreaded this course. Having delighted in biochemistry during his graduate training at Stanford, he was determined to put…

Wood, William B.

2002-01-01

222

Verification of Bohr's frequency condition and Moseley's law: An undergraduate laboratory experiment  

Microsoft Academic Search

We describe an undergraduate laboratory experiment to verify Bohr's frequency condition and Moseley's law using a thin NaI(Tl) detector spectrometer and a weak 57Co source. The slope of the plot of Kalpha x-ray energy versus (Z-1)2 yields a value for the Rydberg constant, R=(1.19+\\/-0.01)×107 m-1, which is in fair agreement with the best literature value, R=10 973 731.534(13) m-1.

S. B. Gudennavar; N. M. Badiger; S. R. Thontadarya; B. Hanumaiah

2003-01-01

223

INTEGRATING COMPUTERIZED DATA ACQUISITION AND ANALYSIS INTO AN UNDERGRADUATE ELECTRIC MACHINES LABORATORY  

Microsoft Academic Search

Abstract ? The Ohio State University (OSU) Department of Electrical Engineering has maintained,an electric machines laboratory,in the undergraduate,curriculum for many,years. Safety has always been of paramount concern and therefore instrumentation,and ,measurement ,procedures ,must ,be developed so as to allow safe operation of the equipment,over a wide range of experimental tests. Hall-effect voltage and current transducers,have been incorporated,into the test table,

Donald G. Kasten

2000-01-01

224

Rigorous Potentiometric Determination of Metal Complexes Stability Constants: An Undergraduate Laboratory Practice  

NASA Astrophysics Data System (ADS)

In the present work an undergraduate laboratory practice is described corresponding to both experimental and computational methods used in potentiometric equilibrium constants determinations. As an example of these determinations the system formed by Cu(II) and D-galacturonic acid was selected. Both the deprotonation constant for the ligand and the equilibrium constants for the metal chelates were determined by using the program BEST.

Escandar, Graciela M.; Federico Sala, Luis

1997-11-01

225

Construction of an Inexpensive Scanning Tunneling Microscope for Undergraduate Laboratories  

NASA Astrophysics Data System (ADS)

Our goal is to produce an inexpensive, room-temperature, atmospheric-pressure scanning tunneling microscope (STM) with atomic resolution. Our prototype uses slip-stick motion for coarse approach to the surface to be imaged. Motion of the tip is accomplished with four flat piezoelectric translators that provide both z-motion (slip-stick and feedback control) as well as the x-y scanning motion. Maximum x-y scan range is estimated and measured to be approximately 1.7 ?m x 1.7 ?m and the fine z-motion range is estimated to be about 570 nm. Control of the x-y motion is done with a microcontroller containing two 16-bit DACs (digital to analog converter). The z-motion is driven by an analog loop that amplifies the tunneling current and then drives the inner electrode of the piezo translators. The cost of our prototype is currently under 300 and we hope to keep it very affordable for high school and college teaching laboratories.

Garcia, Paul; Nitz, Justin; Plano Clark, Mark; Enders, Axel

2009-11-01

226

The Scanning Electron Microscope As An Accelerator For The Undergraduate Advanced Physics Laboratory  

SciTech Connect

Few universities or colleges have an accelerator for use with advanced physics laboratories, but many of these institutions have a scanning electron microscope (SEM) on site, often in the biology department. As an accelerator for the undergraduate, advanced physics laboratory, the SEM is an excellent substitute for an ion accelerator. Although there are no nuclear physics experiments that can be performed with a typical 30 kV SEM, there is an opportunity for experimental work on accelerator physics, atomic physics, electron-solid interactions, and the basics of modern e-beam lithography.

Peterson, Randolph S. [Department of Physics and Astronomy, University of the South, 735 University Avenue, Sewanee TN 37383 (United States); Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 50 Vassar Street, Cambridge MA 01239 (United States); Berggren, Karl K.; Mondol, Mark [Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 50 Vassar Street, Cambridge MA 01239 (United States)

2011-06-01

227

Television Medical Dramas as Case Studies in Biochemistry  

ERIC Educational Resources Information Center

|Several case studies from popular television medical dramas are described for use in an undergraduate biochemistry course. These cases, which illustrate fundamental principles of biochemistry, are used as the basis for problems that can be discussed further in small groups. Medical cases provide an interesting context for biochemistry with video…

Millard, Julie T.

2009-01-01

228

Quality Control Laboratory Design Project for Poultry Science Undergraduate Students Enrolled in an Advanced Food Microbiology Course  

Microsoft Academic Search

An exercise for designing a quality control laboratory was developed as a laboratory group project in a senior level undergraduate advanced food microbiology course. The assignment was based on the student’s designing their own laboratory and implementing testing methods for different types of bacteria known to cause food-borne illness. Individual research papers and group project participation were required of each

R. S. Dittmar; M. M. Kundinger; C. L. Woodward; L. M. Donalson; J. L. Golbach; W. K. Kim; V. Chalova; S. C. Ricke

2006-01-01

229

CLINICAL BIOCHEMISTRY  

EPA Science Inventory

Assessment of the health status of animals through measurement of cellular, biochemical, and macromolecular constituents in blood, secretions, and excretions has been variously referred to as clinical chemistry, clinical biochemistry, or clinical pathology. he genesis of this dis...

230

Chemical Analysis of Soils: An Environmental Chemistry Laboratory for Undergraduate Science Majors  

NASA Astrophysics Data System (ADS)

Successful management of conflicting uses of soils, which include food production as well as disposal of hazardous wastes, requires an understanding of soil characteristics that affect the mobility and fate of soil contaminants and nutrients. Soil chemistry, however, is often neglected in environmental chemistry courses. This laboratory exercise is designed to make this topic more accessible to instructors who teach environmental chemistry laboratories, as well as to their students. In this laboratory exercise, undergraduate science students evaluate soil samples for various parameters related to suitability for crop production and capability for retention of contaminants. The lab emphasizes the heterogeneous nature of soil and the difficulty of obtaining representative samples for analysis. One of the first steps in any chemical analysis is to obtain a representative analytical sample from a bulk sample, yet undergraduate students rarely do this in practice. In this lab, students attempt to obtain a representative analytical sample from a large soil sample. They compare their individual data with the mean and standard deviation compiled from the whole class. Soil water, calcium carbonate, organic matter, pH, and salinity are measured. Soil texture and octanol-water partitioning are demonstrated.

Willey, Joan D.; Avery, G. Brooks, Jr.; Manock, John J.; Skrabal, Stephen A.; Stehman, Charles F.

1999-12-01

231

How Are Humans Related to Other Primates?: A Guided Inquiry Laboratory for Undergraduate Students  

PubMed Central

Understanding that phylogenies depict the evolutionary history of species is a critical concept for undergraduate biology students. We present an inquiry-based laboratory exercise exploring this concept in the context of the human phylogeny. This activity reinforces several important biological concepts and skills. Bolstered concepts include that evolution is descent with modification, that evolution is a genetic process, and that humans are closely related to apes. In terms of thinking skills, the lab gives students practice with hypothetical-deductive thinking, quantifying patterns from complex data, and evaluating evidence.

Kalinowski, Steven T.; Taper, Mark L.; Metz, Anneke M.

2006-01-01

232

Microwave-Enhanced Organic Syntheses for the Undergraduate Laboratory: Diels-Alder Cycloaddition, Wittig Reaction, and Williamson Ether Synthesis  

ERIC Educational Resources Information Center

|Microwave heating enhanced the rate of three reactions typically performed in our undergraduate organic chemistry laboratory: a Diels-Alder cycloaddition, a Wittig salt formation, and a Williamson ether synthesis. Ninety-minute refluxes were shortened to 10 min using a laboratory-grade microwave oven. In addition, yields improved for the Wittig…

Baar, Marsha R.; Falcone, Danielle; Gordon, Christopher

2010-01-01

233

"Anisakis Simplex" Infection in Mackerel: A Reliable Laboratory Exercise to Demonstrate Important Principles in Parasitology to Undergraduates  

ERIC Educational Resources Information Center

|Practical laboratory work in parasitology can be very limited, due to the difficulty in maintaining multi-host parasite life cycles, especially for a large, once-yearly undergraduate laboratory class for life science students. The use of mackerel, "Scomber scombrus," bought from a local fishmonger, is an ideal model to investigate important…

Coombs, I.; Tatner, M.; Paterson, V.

2013-01-01

234

So These Numbers Really Mean Something? A Role Playing Scenario-Based Approach to the Undergraduate Instrumental Analysis Laboratory  

ERIC Educational Resources Information Center

|A new curricular approach in our undergraduate second-year instrumental analysis laboratory was implemented. Students work collaboratively on scenarios in diverse fields including pharmaceuticals, forensics, gemology, art conservation, and environmental chemistry. Each laboratory section (approximately 12 students) is divided into three groups…

Grannas, Amanda M.; Lagalante, Anthony F.

2010-01-01

235

Development of Hands-On Student Experience with Modern Facilities, Measurement Systems, and Uncertainty Analysis in Undergraduate Fluids Engineering Laboratories  

Microsoft Academic Search

Development described of hands-on student experience with modern facilities, measurement systems, and uncertainty analysis in undergraduate fluids engineering laboratories. Classroom and pre-lab lectures and laboratories teach students experimental fluid dynamics (EFD) methodology and uncertainty analysis (UA) procedures following a step-by-step approach, which mirrors the \\

Fred Stern; Marian Muste; Surageet Ghosh; Jun Shao; Don Yarbrough

236

Innovations in Inquiry-Based Laboratory Exercises for Non-Majors Astronomy Courses: Connecting Undergraduates with the Enterprise of Science  

Microsoft Academic Search

Connecting Undergraduates to the Enterprise of Science (CUES) is an NSF-funded program which aims to help instructors towards inquiry based laboratory experiences for students. While many science instructors want to move away from the traditional cookbook laboratories, there are many challenges to executing such a change. The CUES program provides an opportunity for instructors to incorporate inquiry, using \\

Angela Speck; L. Ruzhitskaya; J. C. Weaver

2009-01-01

237

The THCME Medical Biochemistry Page  

NSDL National Science Digital Library

Provided by Dr. Michael W. King of the Terre Haute Center for Medical Education, this stellar resource offers a wealth of biochemistry information, from the basic chemistry of biomolecules to Glycosaminoglycans and mucopolysaccharides. Designed with the undergraduate in mind, each section contains full-text descriptions of a particular subject with accompanying illustrations, tables, figures, and references. Users looking for further information on any topic can use the MedHunt search engine from the front page here to search multiple online medical sources. For any student of biochemistry, this resource will be a valuable asset.

238

Developing Laboratory Facilities for Graduate Research and Undergraduate Studies in Surface Science  

NASA Astrophysics Data System (ADS)

We report current progress and results in an ongoing laboratory development project. This project provides instruments for graduate thesis research in physics and materials science, and training in surface science for advanced undergraduates majoring in the physical sciences and engineering. Two laboratories have been in preparation. One of these labs is now complete, containing an upgraded Phi 560 ESCA/SAMS system for materials surface analysis using Auger electron spectroscopy, X-ray photoelectron spectroscopy, and secondary ion mass spectrometry. The other lab is an ultrahigh vacuum system for low energy diffraction and temperature programmed desorption; it is under development, to be completed upon delivery of a differentially pumped sample manipulator that allows for in situ sample heating to 1200 ^oC and cooling to liquid nitrogen temperatures. The success of the project so far is reflected in the fact that the demand for the use of the upgraded Phi 560 system has exceeded expectations. We give brief descriptions of ongoing research and training involving both graduate and undergraduate students, some results obtained to date, and our aspirations for the future.

Durrer, William; Lopez, Jorge

2003-10-01

239

Optical fibre communications and sensing system experiments for undergraduate photonics laboratories  

NASA Astrophysics Data System (ADS)

Experiments in photonics tend to be reserved for postgraduate laboratories, where suitable equipment and resources are available. Simple optical fibre experiments may be included in some undergraduate programs, possibly utilising polymer optical fibres with LEDs and phototransistors, or with the use of bulk optical components and glass optical fibre elements. However, real optical fibre communication systems and optical fibre sensing systems utilise more complex devices, such as optical fibre Bragg gratings. With the availability of optical components in the 850nm wavelength range, a variety of practical systems can be realised using industry standard components. We show how to mitigate a large portion of the cost associated with the implementation of experiments utilising these 850nm components. The limiting factor associated with the implementation of 1550nm based systems is the cost associated with spectral measurements in this wavelength range. Given a bench top optical spectrum analyser costs $10,000s; this is not something that can be made available to students in undergraduate laboratories in bulk. The solution was to make use of the new low cost USB based spectrometers, available from a number of manufacturers. In combination with devices such as couplers, circulators, isolators, wavelength division multiplexing filters, and Bragg gratings, all operating in the 850nm, a number of different sensing and communications systems can be realised.

Wild, Graham; Swan, Geoff I.

2011-12-01

240

Student perceptions of an upper-level, undergraduate human anatomy laboratory course without cadavers.  

PubMed

Several programs in health professional education require or are considering requiring upper-level human anatomy as prerequisite for their applicants. Undergraduate students are confronted with few institutions offering such a course, in part because of the expense and logistical issues associated with a cadaver-based human anatomy course. This study describes the development of and student reactions to an upper-level human anatomy laboratory course for undergraduate students that used a regional approach and contemporary, alternative teaching methods to a cadaver-based course. The alternative pedagogy to deliver the curriculum included use of commercially available, three-dimensional anatomical virtual dissection software, anatomical models coupled with a learning management system to offer Web-based learning, and a new laboratory manual with collaborative exercises designed to develop the student's anatomical skills and collaborative team skills. A Likert-scale survey with open-ended questions was used to ascertain student perceptions of the course and its various aspects. Students perceived that the noncadaver-based, upper-level human anatomy course with an engaging, regional approach is highly valuable in their learning of anatomy. anatomy. PMID:22362500

Wright, Shirley J

2012-02-23

241

The Academic Majors and Baccalaureate Origins of Biochemistry Graduate Students--A Viewpoint from the USA.  

ERIC Educational Resources Information Center

Reports on a national survey designed to determine some of the ways in which students enter biochemistry programs. Results indicated that the majority of students entering graduate biochemistry programs have an undergraduate biology degree. (TW)

Boyer, Rodney F.

1988-01-01

242

Design for a Simple and Inexpensive Cylinder-within-a-Cylinder Gradient Maker for the Undergraduate Biochemistry Laboratory  

ERIC Educational Resources Information Center

|A design for a simple and inexpensive gradient maker is described. The gradient maker is assembled by (i) cutting the tops off two plastic bottles of differing diameters to produce two cylinders with intact bottoms; (ii) drilling a small hole toward the bottom of the smaller diameter cylinder and plugging the hole with a size 00 cork stopper; and…

Sims, Paul A.; O'Mealey, Gary B.; Khan, Nabeel A.; Larabee, Chelsea M.

2011-01-01

243

Designing Laboratory Exercises for the Undergraduate Molecular Biology/Biochemistry Student: Techniques and Ethical Implications Involved in Personalized Medicine  

ERIC Educational Resources Information Center

|Personalized medicine refers to medical care that involves genetically screening patients for their likelihood to develop various disorders. Commercial genome screening only involves identifying a consumer's genotype for a few single nucleotide polymorphisms. A phenotype (such as an illness) is greatly influenced by three factors: genes, gene…

Weinlander, Kenneth M.; Hall, David J.

2010-01-01

244

Artificial Biochemistry  

Microsoft Academic Search

We model chemical and biochemical systems as collectives of interacting stochastic automata, with each automaton representing\\u000a a molecule that undergoes state transitions. In this artificial biochemistry, automata interact by the equivalent of the law of mass action. We investigate several simple but intriguing automata collectives\\u000a by stochastic simulation and by ODE analysis.

Luca Cardelli

2009-01-01

245

Safety in the Chemical Laboratory: An Undergraduate Chemical Laboratory Safety Course.  

ERIC Educational Resources Information Center

Describes a two-quarter hour college chemistry course focusing on laboratory safety. Includes lists of topics/assignments, problem sets (toxicology, storage, and energy) and videotapes, films, and slide sets used in the course. (JN)

Nicholls, L. Jewel

1982-01-01

246

Safety in the Chemical Laboratory: An Undergraduate Chemical Laboratory Safety Course.  

ERIC Educational Resources Information Center

|Describes a two-quarter hour college chemistry course focusing on laboratory safety. Includes lists of topics/assignments, problem sets (toxicology, storage, and energy) and videotapes, films, and slide sets used in the course. (JN)|

Nicholls, L. Jewel

1982-01-01

247

Promoting Professional Development in Undergraduate Engineering Using Laboratory Team Projects: A Case Study  

NSDL National Science Digital Library

Good professional communication skills and the ability to work in teams are critical skills demanded by engineering companies. Further, in order to maintain competitiveness and to work in the global marketplace, engineering companies are increasingly supporting the creation and use of professional standards that are developed by engineering societies. Unfortunately, many undergraduates have historically received little or no education regarding this aspect of engineering. This paper describes the efforts to provide students with laboratory-based projects which provide students with significant exposure to professional engineering standards and practices. These projects require the students to read professional standards that pertain to the particular topic under study and to operate vehicles while using professional automotive engineering tools.

Davis, Gregory; Hoff, Craig

2011-06-27

248

Design and Construction of a Two-Temperature Preference Behavioral Assay for Undergraduate Neuroscience Laboratories  

PubMed Central

Behavioral assays in the undergraduate neuroscience laboratory are useful for illustrating a variety of physiological concepts. An example is homeostatic temperature regulation (thermoregulation). Many model organisms, from flies to mice, regulate internal temperatures in part by moving to suitable climates (thermotaxis). A particularly reliable method of quantifying temperature-dependent thermotactic behaviors is the two-temperature preference behavioral assay. In this preparation, an organism is free to move between two temperature-controlled surfaces, thus revealing its preferred thermal environment. Here we present the design and construction of a two-temperature preference assay chamber. The device uses Peltier-based thermoelectric modules (TECs) for heating and cooling, and is capable of precision control of temperatures from ?5ºC to 60ºC. Our approach can be easily adapted for use in a variety of physiological and behavioral assays that require precise temperature control over a wide range of temperatures.

Daniels, Richard L.; McKemy, David D.

2010-01-01

249

The Synthesis and Analysis of Ammine Complexes of Copper and Silver Sulfate: An Undergraduate Laboratory Project  

NASA Astrophysics Data System (ADS)

An undergraduate chemistry laboratory project involving the synthesis of [Ag(NH3)2]2SO4 and Cu(NH3)4SO4 from the simple sulfate salts is described. Characterization of the stoichiometry of the complexes is accomplished by gravimetric and volumetric analysis. Silver is precipitated as the chloride and copper is precipitated as the hydroxide and converted to the oxide before weighing. The ammonia content of each complex is determined with a back titration using dilute nitric acid and sodium hydroxide. Typical student work gives results for the stoichiometry of the complexes within 1-5% of the theoretical values. The project provides a convenient preparation of two compounds whose identity and formula will not be obvious to the student. The analytical procedures illustrate the fundamentals of gravimetric and volumetric analysis and some basic characteristics of simple coordination complexes. The analytical data allow the student to determine the identity of each product by determining its empirical formula.

Clareen, Steven S.; Marshall, Shireen R.; Price, Kristin E.; Royall, Margaret B.; Yoder, Claude H.; Schaeffer, Richard W.

2000-07-01

250

Electroretinograms in Drosophila: a robust and genetically accessible electrophysiological system for the undergraduate laboratory.  

PubMed

Laboratory courses in neurophysiology fulfill a critical need for inquiry-based training in undergraduate programs in neuroscience and biology. These courses typically use classical electrophysiological preparations to explore the basic features of neuronal function. However, current neuroscience research also focuses on elucidating the molecular and genetic mechanisms of neuronal function, using model systems that include mutant and transgenic animals. To bridge laboratory training in neurophysiology with modern molecular genetics, we describe a teaching model based on electroretinography of the fruit fly Drosophila melanogaster, a long-established model system for basic neuroscience research. Drosophila are easily maintained, economical, and have hundreds of neurophysiologically relevant mutant strains and genetic tools readily available. The Drosophila electroretinogram (ERG) is a simple and accessible extracellular recording of a neural signal in the fly eye in response to flashes of light. The signal is multifaceted and the response is sensitive to stimulation parameters such as intensity, duration and wavelength, thus forming a rich source of analysis for students. Most importantly, different mutations affecting key components of intracellular signaling, synaptic transmission or neuronal function can affect the ERG waveform in characteristic ways. Recording wild type and mutant ERGs allows students to examine firsthand the connection between genetics, biochemical pathways, and electrophysiology. This neurophysiology laboratory course can facilitate and enhance an understanding of the cellular and molecular contributions to neurophysiological recordings. PMID:23494679

Vilinsky, Ilya; Johnson, Karl G

2012-10-15

251

The Cyclohexanol Cycle and Synthesis of Nylon 6,6: Green Chemistry in the Undergraduate Organic Laboratory  

ERIC Educational Resources Information Center

|A one-term synthesis project that incorporates many of the principles of green chemistry is presented for the undergraduate organic laboratory. In this multistep scheme of reactions, students react, recycle, and ultimately convert cyclohexanol to nylon 6,6. The individual reactions in the project employ environmentally friendly methodologies, and…

Dintzner, Matthew R.; Kinzie, Charles R.; Pulkrabek, Kimberly; Arena, Anthony F.

2012-01-01

252

Acid-Catalyzed Preparation of Biodiesel from Waste Vegetable Oil: An Experiment for the Undergraduate Organic Chemistry Laboratory  

ERIC Educational Resources Information Center

|This undergraduate organic laboratory exercise involves the sulfuric acid-catalyzed conversion of waste vegetable oil into biodiesel. The acid-catalyzed method, although inherently slower than the base-catalyzed methods, does not suffer from the loss of product or the creation of emulsion producing soap that plagues the base-catalyzed methods…

Bladt, Don; Murray, Steve; Gitch, Brittany; Trout, Haylee; Liberko, Charles

2011-01-01

253

LETTERS AND COMMENTS: Permeability measurements in undergraduate vacuum laboratories: a simple experiment using a He leak detector  

Microsoft Academic Search

We describe a simple experiment intended for didactic laboratory vacuum classes of undergraduate courses, using a helium leak detector. The helium throughput flowing into the vacuum volume due to the permeability of materials can be taken as a real leak, which can be measured with the helium leak detector. The experiment allows students to perform actual measurements of helium permeability

J. M. F. dos Santos; J. F. C. A. Veloso; C. M. B. Monteiro

2004-01-01

254

Assembly of a Modular Fluorimeter and Associated Software: Using LabVIEW in an Advanced Undergraduate Analytical Chemistry Laboratory  

ERIC Educational Resources Information Center

|A laboratory activity for an upper-level undergraduate course in instrumental analysis has been created around LabVIEW. Students learn rudimentary programming and interfacing skills during the construction of a fluorimeter assembled from common modular components. The fluorimeter consists of an inexpensive data acquisition module, LED light…

Algar, W. Russ; Massey, Melissa; Krull, Ulrich J.

2009-01-01

255

Gold Electrodes Modified with Self-Assembled Monolayers for Measuring L-Ascorbic Acid: An Undergraduate Analytical Chemistry Laboratory Experiment  

ERIC Educational Resources Information Center

|This article describes an undergraduate electrochemistry laboratory experiment in which the students measure the L-ascorbic acid content of a real sample. Gold electrodes modified with self-assembled monolayers (SAMs) of thioctic acid and cysteamine are prepared to study the effects of surface modification on the electrode reaction of L-ascorbic…

Ito, Takashi; Perera, D. M. Neluni T.; Nagasaka, Shinobu

2008-01-01

256

Isolation and Culture of Bovine Oviductal Epithelial Cells for Use in the Anatomy and Physiology Laboratory and Undergraduate Research  

ERIC Educational Resources Information Center

|This article presents methods for the isolation and culture of epithelial cells from the bovine oviduct for use in both research and the teaching laboratory and provides examples of ways that an oviductal cell culture can be incorporated into an undergraduate research program. Cow reproductive tracts are readily available from area butchers, and…

Way, Amy L.

2006-01-01

257

Validating the Goldstein-Wehner Law for the Stratified Positive Column of DC Discharge in an Undergraduate Laboratory  

ERIC Educational Resources Information Center

|In this paper we suggest a simple technique for validating the Goldstein-Wehner law for a stratified positive column of dc glow discharge while studying the properties of gas discharges in an undergraduate laboratory. To accomplish this a simple device with a pre-vacuum mechanical pump, dc source and gas pressure gauge is required. Experiments…

Lisovskiy, V. A.; Koval, V. A.; Artushenko, E. P.; Yegorenkov, V. D.

2012-01-01

258

SIPCAn (Separation, Isolation, Purification, Characterization, and Analysis): A One-Term, Integrated Project for the Undergraduate Organic Laboratory  

ERIC Educational Resources Information Center

SIPCAn, an acronym for separation, isolation, purification, characterization, and analysis, is presented as a one-term, integrated project for the first-term undergraduate organic laboratory course. Students are assigned two mixtures of unknown organic compounds--a mixture of two liquid compounds and a mixture of two solid compounds--at the…

Dintzner, Matthew R.; Kinzie, Charles R.; Pulkrabek, Kimberly A.; Arena, Anthony F.

2011-01-01

259

An Undergraduate Laboratory Exercise to Study the Effect of Darkness on Plant Gene Expression Using DNA Microarray  

ERIC Educational Resources Information Center

|DNA microarrays are microscopic arrays on a solid surface, typically a glass slide, on which DNA oligonucleotides are deposited or synthesized in a high-density matrix with a predetermined spatial order. Several types of DNA microarrays have been developed and used for various biological studies. Here, we developed an undergraduate laboratory

Chang, Ming-Mei; Briggs, George M.

2007-01-01

260

The Cyclohexanol Cycle and Synthesis of Nylon 6,6: Green Chemistry in the Undergraduate Organic Laboratory  

ERIC Educational Resources Information Center

A one-term synthesis project that incorporates many of the principles of green chemistry is presented for the undergraduate organic laboratory. In this multistep scheme of reactions, students react, recycle, and ultimately convert cyclohexanol to nylon 6,6. The individual reactions in the project employ environmentally friendly methodologies, and…

Dintzner, Matthew R.; Kinzie, Charles R.; Pulkrabek, Kimberly; Arena, Anthony F.

2012-01-01

261

Oxidation of Borneol to Camphor Using Oxone and Catalytic Sodium Chloride: A Green Experiment for the Undergraduate Organic Chemistry Laboratory  

ERIC Educational Resources Information Center

|A new green oxidation procedure was developed for the undergraduate organic teaching laboratories using Oxone and a catalytic quantity of sodium chloride for the conversion of borneol to camphor. This simple 1 h, room temperature reaction afforded high quality and yield of product, was environmentally friendly, and produced negligible quantities…

Lang, Patrick T.; Harned, Andrew M.; Wissinger, Jane E.

2011-01-01

262

An Environmentally Significant Experiment Using GC\\/MS and GC Retention Indices in an Undergraduate Analytical Laboratory  

Microsoft Academic Search

An undergraduate laboratory experiment has been developed in which the students extract and analyze the additives from plastic food wrap, Tygon tubing and plastic soda bottles that have been crushed in a bottle return machine. The modifiers are extracted from the plastics using methylene chloride and\\/or methanol as solvents and the extracts are analyzed by GC\\/MS. Identifications are carried out

Rebecca Guisto-Norkus; Giv Gounili; Peter Wisniecki; John A. Hubball; S. Ruven Smith; James D. Stuart

1996-01-01

263

Red Seaweed Enzyme-Catalyzed Bromination of Bromophenol Red: An Inquiry-Based Kinetics Laboratory Experiment for Undergraduates  

ERIC Educational Resources Information Center

|Haloperoxidase enzymes are of interest for basic and applied bioscientists because of their increasing importance in pharmaceutical industry and environmental cleanups. In a guided inquiry-based laboratory experiment for life-science, agricultural science, and health science undergraduates, the bromoperoxidase from a red seaweed was used to…

Jittam, Piyachat; Boonsiri, Patcharee; Promptmas, Chamras; Sriwattanarothai, Namkang; Archavarungson, Nattinee; Ruenwongsa, Pintip; Panijpan, Bhinyo

2009-01-01

264

SIPCAn (Separation, Isolation, Purification, Characterization, and Analysis): A One-Term, Integrated Project for the Undergraduate Organic Laboratory  

ERIC Educational Resources Information Center

|SIPCAn, an acronym for separation, isolation, purification, characterization, and analysis, is presented as a one-term, integrated project for the first-term undergraduate organic laboratory course. Students are assigned two mixtures of unknown organic compounds--a mixture of two liquid compounds and a mixture of two solid compounds--at the…

Dintzner, Matthew R.; Kinzie, Charles R.; Pulkrabek, Kimberly A.; Arena, Anthony F.

2011-01-01

265

Preparation of a D-Glucose-Derived Alkene. An E2 Reaction for the Undergraduate Organic Chemistry Laboratory  

Microsoft Academic Search

As the carbohydrates continue to gain attention for their central role in numerous areas of chemistry and biology, there is a growing need for experiments at the undergraduate level that highlight these fascinating compounds. In this article we describe a simple two-step laboratory experiment that may be used to highlight several important concepts introduced in sophomore organic chemistry, as applied

Peter Norris; Andrew Fluxe

2001-01-01

266

The use of multiple tools for teaching medical biochemistry  

NSDL National Science Digital Library

In this work, we describe the use of several strategies employing the philosophies of active learning and problem-based learning (PBL) that may be used to improve the teaching of metabolic biochemistry to medical and nutritional undergraduate students.

Alexandre B. Sé (Universidade de Brasília Oxyradical Research Group, Departamento de Biologia Celular); Renato M. Passos (Universidade de Brasília Oxyradical Research Group, Departamento de Biologia Celular); Andre H Ono (Universidade de Brasilia Medicina); Marcelo Hermes-Lima (Universidade de Brasília Oxyradical Research Group, Departamento de Biologia Celular)

2007-07-27

267

Glucose Transport in Cultured Animal Cells: An Exercise for the Undergraduate Cell Biology Laboratory  

PubMed Central

Membrane transport is a fundamental concept that undergraduate students of cell biology understand better with laboratory experience. Formal teaching exercises commonly used to illustrate this concept are unbiological, qualitative, or intricate and time consuming to prepare. We have developed an exercise that uses uptake of radiolabeled nutrient analogues by attachment-dependent animal cells cultured on multiwell trays. This system can readily be manipulated within a typical 3-h laboratory period to yield reproducible, biologically relevant, quantitative data regarding key aspects of membrane transport. Each 24-well tray of cultures allows a group of two to four students to compare eight conditions in triplicate. If different groups of students test different conditions or different types of cells, data can be shared for an even broader experience. The exercise is also readily adaptable for open-ended student projects. Here we illustrate the exercise measuring uptake of the nonmetabolizable glucose analogue [3H]-2-deoxy-d-glucose. Students successfully tested the effects of competing sugars, putative inhibitors of the GLUT1 transporter, and changes in cell physiology that might be expected to affect glucose transport in epithelial cells and fibroblasts. In this exercise students find the nutritional and medical implications of glucose transport and its regulation intriguing. They also learn to handle radioisotopes and cultured cells.

Ledbetter, Mary Lee S; Lippert, Malcolm J

2002-01-01

268

Curriculum modules, software laboratories, and an inexpensive hardware platform for teaching computational methods to undergraduate computer science students  

NASA Astrophysics Data System (ADS)

Computational methods are increasingly important to 21st century research and education; bioinformatics and climate change are just two examples of this trend. In this context computer scientists play an important role, facilitating the development and use of the methods and tools used to support computationally-based approaches. The undergraduate curriculum in computer science is one place where computational tools and methods can be introduced to facilitate the development of appropriately prepared computer scientists. To facilitate the evolution of the pedagogy, this dissertation identifies, develops, and organizes curriculum materials, software laboratories, and the reference design for an inexpensive portable cluster computer, all of which are specifically designed to support the teaching of computational methods to undergraduate computer science students. Keywords. computational science, computational thinking, computer science, undergraduate curriculum.

Peck, Charles Franklin

269

Teaching the Fundamentals of Pulsed NMR Spectroscopy in an Undergraduate Physical Chemistry Laboratory  

Microsoft Academic Search

Pulsed nuclear magnetic resonance (NMR) spectroscopy has become the premiere technique for chemists and biochemists studying the conformational, structural, and dynamic properties of a wide variety of molecular and biological systems. The theories and physical concepts associated with pulsed NMR spectroscopy are difficult for undergraduate students to comprehend and often require a thorough understanding of spin physics. Additionally, undergraduates in

Gary A. Lorigan; Robert E. Minto; Wei Zhang

2001-01-01

270

Demand for Interdisciplinary Laboratories for Physiology Research by Undergraduate Students in Biosciences and Biomedical Engineering  

ERIC Educational Resources Information Center

|Physiology as a discipline is uniquely positioned to engage undergraduate students in interdisciplinary research in response to the 2006-2011 National Science Foundation Strategic Plan call for innovative transformational research, which emphasizes multidisciplinary projects. To prepare undergraduates for careers that cross disciplinary…

Clase, Kari L.; Hein, Patrick W.; Pelaez, Nancy J.

2008-01-01

271

Experimental determination of the Boltzmann constant: An undergraduate laboratory exercise for molecular physics or physical chemistry  

NASA Astrophysics Data System (ADS)

This article describes an undergraduate laboratory exercise that uses optical spectroscopy to determine the magnitude and the uncertainty of the Boltzmann constant kb. The more accurate approach uses photoacoustic spectroscopy to measure the Doppler-broadened line profile of individual spectral lines of N2O to extract kb. Measurements and estimates of the uncertainties in the quantities needed to calculate kb from the line profiles are then used to estimate the uncertainty in kb. This experiment is unusual in that it uses advanced laser-based spectroscopy techniques to emphasize standard practices of uncertainty analysis. The core instrumentation is modular and relatively affordable; it requires a tunable single-mode laser, photoreceiver, optical cell, and vacuum pump. If this instrumentation is not available, an alternate approach can be performed which uses the intensity of each rotational transition of an infrared band to measure kb. Although there is more uncertainty using the alternate approach, low concentrations of CO2, DCl, or N2O give reasonable results for the magnitude of kb. Student assessment results indicate retention and mastery of the concept of combined measurement uncertainty.

Campbell, H. M.; Boardman, B. M.; DeVore, T. C.; Havey, D. K.

2012-12-01

272

Testing plastic deformations of materials in the introductory undergraduate mechanics laboratory  

NASA Astrophysics Data System (ADS)

Normally, a mechanics laboratory at the undergraduate level includes an experiment to verify compliance with Hooke's law in materials, such as a steel spring and an elastic rubber band. Stress-strain curves are found for these elements. Compression in elastic bands is practically impossible to achieve due to flaccidity. A typical experiment for the complete loading-unloading cycle is to subject a tubular object to torsion. This paper suggests simple experiments for studying properties concerning elasticity and plasticity in elements of common use, subjected to stretching or compression, and also torsion reinforcing. The experiments use plastic binders, rubber bands and metal springs under a moderate load. This paper discusses an experiment with an original device to measure torsion deformations as a function of applied torques, which permitted construction of the hysteresis cycle for a rubber hose and various tubes. Another experiment was designed to define the temporal recovery of a plastic spring with initial stretching. A simple mathematical model was developed to explain this phenomenon.

Romo-Kröger, C. M.

2012-05-01

273

The effects of inquiry instruction on student learning in technology-based undergraduate chemistry laboratories  

NASA Astrophysics Data System (ADS)

The purpose of this study was to identify conceptual and attitudinal effects of inquiry learning in technology-based undergraduate chemistry laboratories. There were 428 participants who were registered in general chemistry laboratory at the University of Iowa in the Spring of 2002. Conceptual and attitudinal pretest and posttest results were quantitative in nature. Qualitative results were collected from questionnaires and focus groups. Quantitative data were analyzed using a repeated measures analysis of variance to identify differences between treatment groups. A high-inquiry treatment group was open-ended and required student decisions regarding data collection, data representation, and interpretation. The low-inquiry treatment involved collaboration and traditional learning strategies. Major findings of this study were: (1) Pretest to posttest conceptual gains were significant for both treatment groups. Low-inquiry students performed significantly better on exploration questions than high-inquiry students. (2) Process skills developed at higher levels for high-inquiry students than low-inquiry students. (3) Positive attitudes decreased significantly for all students from pretest to posttest. More favorable attitudes toward science enjoyment and the ability to do well in science were found for high-inquiry students. More favorable attitudes toward science enjoyment and the ability to do well in science were found for low-inquiry males and high-inquiry females. (4) More favorable attitudes toward the nature of science caused by use of the learning cycle were reported by high-inquiry students. (5) Low-inquiry students reported more favorable attitudes toward technologies in the laboratory than did high-inquiry students. Favorable attitudes toward the use of infrared spectrometers and unfavorable attitudes toward the use of pH meters were reported by both treatment groups. (6) More formal reasoning skills were reported by high-inquiry students. Both groups reported that looking for patterns was a common theme in the laboratories. Hypotheses were reported as rarely used by both treatment groups. These findings are significant because they indicate that inquiry activities positively affect attitudes toward science, gender equality, and contribute to the development of formal reasoning skills and process skills.

Meade, Karen Marie

274

Experimental Methods in Neuroscience: An Undergraduate Neuroscience Laboratory Course for Teaching Ethical Issues, Laboratory Techniques, Experimental Design, and Analysis  

PubMed Central

We have developed and recently taught a 200 level undergraduate course entitled, ‘Experimental Methods in Neuroscience’. This is a required course in an increasingly popular Neuroscience major at Smith College. Students are introduced initially to issues of animal ethics and experimentation, and are familiarized with our Animal Care Facility. Using an open field and rotarod apparatus, and the elevated plus and Barnes mazes, they conduct behavioral testing of two strains of mice, C57/BL/6J and 129S1/SvImJ, known to exhibit distinct behavioral traits. The group then employs histological techniques to prepare brain sections for observing neuroanatomical variation between strains (for example, 129S1/SvImJ mice are occasionally acallosal). In the final laboratory exercise, they assay the acetylcholinesterase activity in fore- and hindbrains from each strain. The experiments enable the students to gain confidence in collecting data, compiling large data sets, handling spreadsheets and graphing, applying appropriate statistics, and writing accurate and concise scientific reports in journal article format. The course concludes with pairs of students conducting self-designed independent projects using the acquired behavioral, histological or neurochemical techniques. Experimental Methods in Neuroscience is proving particularly successful as it is relatively straightforward for students to design interesting experiments, gain experience in neuroscience experimentation without excessive use of animals, gather substantial data sets, and develop skills in scientific report writing and presentation at an early stage in their neuroscience curricula. Furthermore, the course has emerged as a centralizing focus for our neuroscience program and is suitable for transfer to and adaptation by other institutions.

Hall, Adam C.; Harrington, Mary E.

2003-01-01

275

Synthesis and Kinetics of Hydrolysis of 3,5-Dimethyl-N-acetyl-p-benzoquinone Imine: An Undergraduate Laboratory  

NASA Astrophysics Data System (ADS)

The synthesis of the title compound by a three-step procedure is described. The hydrolysis kinetics, which involve two consecutive psuedo-first-order processes, are also described. The synthesis and kinetics experiments described here are proposed for incorporation into undergraduate laboratory courses under a variety of formats. The compound described here is related to a toxic metabolite of the common analgesics acetaminophen and phenacetin.

Buccigross, Jeanne M.; Metz, Christa; Elliot, Lori; Becker, Pamela; Earley, Angela S.; Hayes, Jerry W.; Novak, Michael; Underwood, Gayl A.

1996-04-01

276

An Environmentally Significant Experiment Using GC/MS and GC Retention Indices in an Undergraduate Analytical Laboratory  

NASA Astrophysics Data System (ADS)

An undergraduate laboratory experiment has been developed in which the students extract and analyze the additives from plastic food wrap, Tygon tubing and plastic soda bottles that have been crushed in a bottle return machine. The modifiers are extracted from the plastics using methylene chloride and/or methanol as solvents and the extracts are analyzed by GC/MS. Identifications are carried out by a combination of automated computer and manual library searches and chromatographic retention indices.

Guisto-Norkus, Rebecca; Gounili, Giv; Wisniecki, Peter; Hubball, John A.; Ruven Smith, S.; Stuart, James D.

1996-12-01

277

Monitoring Hammerhead Ribozyme-Catalyzed Cleavage with a Fluorescein-Labeled Substrate: Effects of Magnesium Ions and Antibiotic Inhibitors. A Biochemistry Laboratory: Part 2  

NASA Astrophysics Data System (ADS)

An experiment is presented that demonstrates current techniques in modern RNA research and introduces a method for nonradioactive monitoring of RNA reactions. The laboratory involves the study of hammerhead ribozyme activity and the influence of metal ions and antibiotics on these important RNA-based reactions. The ribozyme class of RNA catalysts has current applications in both biotechnology and medicine and therefore should be of great interest to upper-level undergraduate students who anticipate careers in these areas. The students gain hands-on experience in working with RNA on a picomole level and also learn about gel electrophoresis, the use of fluorescent tagging, RNA-small molecule interactions, and the role of metal ions in biological systems. This laboratory offers students an opportunity to work with molecules that have direct applications in drug therapy and RNA catalysis.

Chow, Christine S.; Somne, Smita; Llano-Sotelo, Beatriz

1999-05-01

278

Development of a Green Fluorescent Protein-Based Laboratory Curriculum  

ERIC Educational Resources Information Center

|A laboratory curriculum has been designed for an undergraduate biochemistry course that focuses on the investigation of the green fluorescent protein (GFP). The sequence of procedures extends from analysis of the DNA sequence through PCR amplification, recombinant plasmid DNA synthesis, bacterial transformation, expression, isolation, and…

Larkin, Patrick D.; Hartberg, Yasha

2005-01-01

279

Application of sigma metrics for the assessment of quality assurance in clinical biochemistry laboratory in India: a pilot study.  

PubMed

Ensuring quality of laboratory services is the need of the hour in the field of health care. Keeping in mind the revolution ushered by six sigma concept in corporate world, health care sector may reap the benefits of the same. Six sigma provides a general methodology to describe performance on sigma scale. We aimed to gauge our laboratory performance by sigma metrics. Internal quality control (QC) data was analyzed retrospectively over a period of 6 months from July 2009 to December 2009. Laboratory mean, standard deviation and coefficient of variation were calculated for all the parameters. Sigma was calculated for both the levels of internal QC. Satisfactory sigma values (>6) were elicited for creatinine, triglycerides, SGOT, CPK-Total and Amylase. Blood urea performed poorly on the sigma scale with sigma <3. The findings of our exercise emphasize the need for detailed evaluation and adoption of ameliorative measures in order to effectuate six sigma standards for all the analytical processes. PMID:22468038

Singh, Bhawna; Goswami, Binita; Gupta, Vinod Kumar; Chawla, Ranjna; Mallika, Venkatesan

2010-11-16

280

Analysis of Currently Available Analgesic Tablets by Modern Liquid Chromatography: An Undergraduate Laboratory Introduction to HPLC.  

ERIC Educational Resources Information Center

|Background information, procedures, and results, are provided for an undergraduate experiment in which analgesic tablets are analyzed using liquid chromatography. The experiment, an improved, modified version of the Waters Associates Inc. experiment, is simple to prepare, requiring little glassware and minimal sample manipulation by students.…

Kagel, R. A.; Farwell, S. O.

1983-01-01

281

Analysis of Currently Available Analgesic Tablets by Modern Liquid Chromatography: An Undergraduate Laboratory Introduction to HPLC.  

ERIC Educational Resources Information Center

Background information, procedures, and results, are provided for an undergraduate experiment in which analgesic tablets are analyzed using liquid chromatography. The experiment, an improved, modified version of the Waters Associates Inc. experiment, is simple to prepare, requiring little glassware and minimal sample manipulation by students. (JN)

Kagel, R. A.; Farwell, S. O.

1983-01-01

282

Use of Microcomputers in the Undergraduate Chemistry Laboratory: An Absorption Spectrum Experiment.  

ERIC Educational Resources Information Center

Describes a computer program designed to enable undergraduate students to apply computer skills for data acquisition and processing in experimental chemistry. An example is given that examines the absorption spectra of conjugated molecules such as carbocyanine dyes, and the free electron model is explained. (six references) (LRW)

Terry, Ronald J.; And Others

1989-01-01

283

Rapid Multistep Synthesis of a Bioactive Peptidomimetic Oligomer for the Undergraduate Laboratory  

ERIC Educational Resources Information Center

Peptidomimetic compounds are increasingly important in drug-discovery applications. We introduce the synthesis of an N-substituted glycine oligomer, a bioactive "peptoid" trimer. The six-step protocol is conducted on solid-phase resin, enabling the synthesis to be performed by undergraduate organic chemistry students. This synthesis lab was…

Utku, Yeliz; Rohatgi, Abhinav; Yoo, Barney; Kirshenbaum, Kent; Zuckermann, Ronald N.; Pohl, Nicola L.

2010-01-01

284

Organic Materials in the Undergraduate Laboratory: Microscale Synthesis and Investigation of a Donor-Acceptor Molecule  

ERIC Educational Resources Information Center

|A series of experiments for undergraduate courses (e.g., organic, physical) have been developed in the area of small molecule organic materials. These experiments focus on understanding the electronic and redox properties of a donor-acceptor molecule that is prepared in a convenient one-step microscale reaction. The resulting intensely colored…

Pappenfus, Ted M.; Schliep, Karl B.; Dissanayake, Anudaththa; Ludden, Trevor; Nieto-Ortega, Belen; Lopez Navarrete, Juan T.; Ruiz Delgado, M. Carmen; Casado, Juan

2012-01-01

285

Verification of Compton Collision and Klein-Nishina Formulas--An Undergraduate Laboratory Experiment  

ERIC Educational Resources Information Center

|Describes an experiment to verify the Compton collision formula and the angular dependance of the Klein-Nishina formula. Equipment used is a 1-mCi(137)Cs source, 2x2 in. NaI detector and a multichannel analyzer. Suitable for honor undergraduates. (Author/GA)|

Singhal, R. P.; Burns, A. J.

1978-01-01

286

Engaging Students in Inquiry: Tales From an Undergraduate Geology Laboratory-Based Course  

ERIC Educational Resources Information Center

This paper reports the synthesis of three case studies of students' engagement in inquiry-based learning activities in an upper-level undergraduate geology course. Details of how students engaged in scientific questions, gave priority to evidence, formulated explanations, evaluated explanations, and communicated and justified their findings are…

Apedoe, Xornam S.

2008-01-01

287

A Writing-Intensive, Methods-Based Laboratory Course for Undergraduates  

ERIC Educational Resources Information Center

Engaging undergraduate students in designing and executing original research should not only be accompanied by technique training but also intentional instruction in the critical analysis and writing of scientific literature. The course described here takes a rigorous approach to scientific reading and writing using primary literature as the model…

Colabroy, Keri L.

2011-01-01

288

Rapid Multistep Synthesis of a Bioactive Peptidomimetic Oligomer for the Undergraduate Laboratory  

ERIC Educational Resources Information Center

|Peptidomimetic compounds are increasingly important in drug-discovery applications. We introduce the synthesis of an N-substituted glycine oligomer, a bioactive "peptoid" trimer. The six-step protocol is conducted on solid-phase resin, enabling the synthesis to be performed by undergraduate organic chemistry students. This synthesis lab was…

Utku, Yeliz; Rohatgi, Abhinav; Yoo, Barney; Kirshenbaum, Kent; Zuckermann, Ronald N.; Pohl, Nicola L.

2010-01-01

289

A Writing-Intensive, Methods-Based Laboratory Course for Undergraduates  

ERIC Educational Resources Information Center

|Engaging undergraduate students in designing and executing original research should not only be accompanied by technique training but also intentional instruction in the critical analysis and writing of scientific literature. The course described here takes a rigorous approach to scientific reading and writing using primary literature as the…

Colabroy, Keri L.

2011-01-01

290

Online Protocol Annotation: A Method to Enhance Undergraduate Laboratory Research Skills  

ERIC Educational Resources Information Center

|A well-constructed, step-by-step protocol is a critical starting point for teaching undergraduates new techniques, an important record of a lab's standard procedures, and a useful mechanism for sharing techniques between labs. Many research labs use websites to archive and share their protocols for these purposes. Here we describe our experiences…

Ruble, Julie E.; Lom, Barbara

2008-01-01

291

A Gas-Sensor-Based Urea Enzyme Electrode: Its Construction and Use in the Undergraduate Laboratory.  

ERIC Educational Resources Information Center

|Describes an undergraduate experiment for the potentiometric determination of urea based on the physical entrapment of urease on the tip of an ammonia gas sensor. An advantage of this technique is the ease with which the ammonia electrode can be converted to a urea electrode. (JN)|

Riechel, Thomas L.

1984-01-01

292

A Cost-Effective Atomic Force Microscope for Undergraduate Control Laboratories  

ERIC Educational Resources Information Center

|This paper presents a simple, cost-effective and robust atomic force microscope (AFM), which has been purposely designed and built for use as a teaching aid in undergraduate controls labs. The guiding design principle is to have all components be open and visible to the students, so the inner functioning of the microscope has been made clear to…

Jones, C. N.; Goncalves, J.

2010-01-01

293

Factors modifying duration of drug action: a practical for large undergraduate classes on limited laboratory space, staff and budget.  

PubMed

A desperate need for health professionals is answered by yearly increases in university student admissions in many developing countries. Yet economic constraints dictate that subventions for teaching and research remain static or even decrease as student populations increase. Practical teaching, for example in pharmacology, is susceptible to inadequate funding because of the capital and recurrent expenditure needed to procure and maintain laboratories, staff, animals, instruments and chemicals. Class demonstrations, although they provide a partial answer to the problem, are beset with a number of disadvantages. Provision of good training on low funds demands ingenuity to modify teaching/learning processes while still achieving the desired objectives. This paper illustrates such a procedure by describing the logistics of a practical class on 'Factors modifying duration of drug action' to large classes of undergraduate medical and pharmacy students, on limited laboratory space, staff and budget'. A sample laboratory result is included. PMID:2796794

Kwanashie, K O; Amabeoku, G J; Auta, J

1989-09-01

294

New Ideas for an Old Enzyme: A Short, Question-Based Laboratory Project for the Purification and Identification of an Unknown LDH Isozyme  

ERIC Educational Resources Information Center

Enzyme purification projects are an excellent way to introduce many aspects of protein biochemistry, but can be difficult to carry out under the constraints of a typical undergraduate laboratory course. We have designed a short laboratory project for the purification and identification of an "unknown" lactate dehydrogenase (LDH) isozyme that can…

Coleman, Aaron B.

2010-01-01

295

New Ideas for an Old Enzyme: A Short, Question-Based Laboratory Project for the Purification and Identification of an Unknown LDH Isozyme  

ERIC Educational Resources Information Center

|Enzyme purification projects are an excellent way to introduce many aspects of protein biochemistry, but can be difficult to carry out under the constraints of a typical undergraduate laboratory course. We have designed a short laboratory project for the purification and identification of an "unknown" lactate dehydrogenase (LDH) isozyme that can…

Coleman, Aaron B.

2010-01-01

296

75 FR 8147 - Notice of Consideration of Amendment Request for Decommissioning of Analytical Bio-Chemistry...  

Federal Register 2010, 2011, 2012, 2013

...Amendment Request for Decommissioning of Analytical Bio-Chemistry Laboratories, Inc. Sanitary Lagoon, Columbia, Missouri...Material License No. 24- 13365-01 issued to Analytical Bio-Chemistry Laboratories, Inc. (the Licensee) pursuant to 10 CFR...

2010-02-23

297

Measurement of the molar fraction of 13C in diamond films using Raman and Rutherford scattering – an undergraduate student laboratory experience  

Microsoft Academic Search

The usual weekly laboratory experiment for the upper-division modern physics lab was replaced with a theme of studying diamond films grown on natural diamond. The diamonds were grown at the University of Alabama at Birmingham and were studied at university physics laboratories at UAB, Alabama A&M University, The University of the South, and the University of North Texas. Undergraduate students

David Matthew Walters; William S. Hooker; Elizabeth W. Shroyer; Paul A. Baker; Yogesh K. Vohra; Randolph S. Peterson

2005-01-01

298

Measurement of the molar fraction of 13C in diamond films using Raman and Rutherford scattering an undergraduate student laboratory experience  

Microsoft Academic Search

The usual weekly laboratory experiment for the upper-division modern physics lab was replaced with a theme of studying diamond films grown on natural diamond. The diamonds were grown at the University of Alabama at Birmingham and were studied at university physics laboratories at UAB, Alabama A&M University, The University of the South, and the University of North Texas. Undergraduate students

David Matthew Walters; William S. Hooker; Elizabeth W. Shroyer; Paul A. Baker; Yogesh K. Vohra; Randolph S. Peterson

2005-01-01

299

Implementing POGIL in the lecture and the Science Writing Heuristic in the laboratory—student perceptions and performance in undergraduate organic chemistry  

Microsoft Academic Search

This study investigated the possible connection between effective laboratory activities and student performance on lecture exams. In a traditional undergraduate organic chemistry course for non- science majors, students could predict the products of organic reactions, but struggled to provide reaction mechanisms for those same reactions, despite obtaining perfect scores on their laboratory reports where reaction mechanisms were required. In addition,

Jacob D. Schroeder; Thomas J. Greenbowe

2008-01-01

300

A Graphical Simulation of Vapor-Liquid Equilibrium for Use as an Undergraduate Laboratory Experiment and to Demonstrate the Concept of Mathematical Modeling.  

ERIC Educational Resources Information Center

|Demonstrating petroleum engineering concepts in undergraduate laboratories often requires expensive and time-consuming experiments. To eliminate these problems, a graphical simulation technique was developed for junior-level laboratories which illustrate vapor-liquid equilibrium and the use of mathematical modeling. A description of this…

Whitman, David L.; Terry, Ronald E.

1985-01-01

301

Determining the Transference Number of H[superscript +](aq) by a Modified Moving Boundary Method: A Directed Study for the Undergraduate Physical Chemistry Laboratory  

ERIC Educational Resources Information Center

|A directed study for the undergraduate physical chemistry laboratory for determining the transference number of H[superscript +](aq) using a modified moving boundary method is presented. The laboratory study combines Faraday's laws of electrolysis with mole ratios and the perfect gas equation. The volume of hydrogen gas produced at the cathode is…

Dabke, Rajeev B.; Gebeyehu, Zewdu; Padelford, Jonathan

2012-01-01

302

Physics 433: Undergraduate laboratory in Biological Physics at Simon Fraser University  

NASA Astrophysics Data System (ADS)

In this talk, I will describe our senior undergraduate lab course in Biological Physics at Simon Fraser University. Unique in Canada, this course combines modules that teach the students basic molecular and cell biology and leading-edge biophysical techniques with independent student projects. I'll outline the structure and components of the course, provide an overview of each experimental module, and describe student interest and feedback.

Forde, Nancy

2012-10-01

303

Automated scanning probe lithography with n-alkanethiol self assembled monolayers on Au(111): Application for teaching undergraduate laboratories  

PubMed Central

Controllers for scanning probe instruments can be programmed for automated lithography to generate desired surface arrangements of nanopatterns of organic thin films, such as n-alkanethiol self-assembled monolayers (SAMs). In this report, atomic force microscopy (AFM) methods of lithography known as nanoshaving and nanografting are used to write nanopatterns within organic thin films. Commercial instruments provide software to control the length, direction, speed, and applied force of the scanning motion of the tip. For nanoshaving, higher forces are applied to an AFM tip to selectively remove regions of the matrix monolayer, exposing bare areas of the gold substrate. Nanografting is accomplished by force-induced displacement of molecules of a matrix SAM, followed immediately by the surface self-assembly of n-alkanethiol molecules from solution. Advancements in AFM automation enable rapid protocols for nanolithography, which can be accomplished within the tight time restraints of undergraduate laboratories. Example experiments with scanning probe lithography (SPL) will be described in this report that were accomplished by undergraduate students during laboratory course activities and research internships in the chemistry department of Louisiana State University. Students were introduced to principles of surface analysis and gained “hands-on” experience with nanoscale chemistry.

Brown, Treva T.; LeJeune, Zorabel M.; Liu, Kai; Hardin, Sean; Li, Jie-Ren; Rupnik, Kresimir; Garno, Jayne C.

2010-01-01

304

Designing Polymerase Chain Reaction (PCR) Primer Multiplexes in the Forensic Laboratory  

ERIC Educational Resources Information Center

|The polymerase chain reaction (PCR) is a common experiment in upper-level undergraduate biochemistry, molecular biology, and forensic laboratory courses as reagents and thermocyclers have become more affordable for institutions. Typically, instructors design PCR primers to amplify the region of interest and the students prepare their samples for…

Elkins, Kelly M.

2011-01-01

305

Simple Laboratory Exercise for Induction of Beta-Mannanase from "Aspergillus niger"  

ERIC Educational Resources Information Center

|This laboratory experiment was designed for Biochemistry, Biotechnology, Microbiology, and Food Technology students of undergraduate and postgraduate courses. The experiment shows the advantages of using agricultural waste, copra mannan as potent inducer of [beta]-mannanase. The students were able to compare the enzyme induction by commercial…

Mulimani, V. H.; Naganagouda, K.

2010-01-01

306

Chemoenzymatic Synthesis of an Enantiomerically Pure Lactone: A Three-Step Synthesis for Undergraduate Organic Chemistry Laboratory  

NASA Astrophysics Data System (ADS)

A three-step laboratory sequence for the undergraduate organic laboratory is described. This series of experiments requires a student to use the product from one reaction as the starting material for a subsequent reaction, and thus the affords the student a "real world" experience of multistep synthesis. Thermal extrusion of sulfur dioxide from sulfolene is used to generate 1,3-butadiene in situ for a Diels-Alder cyclization with maleic anhydride. The anhydride is then reduced to the diol with lithium aluminum hydride. Oxidation of the diol to the chiral lactone is catalyzed by horse-liver alcohol dehydrogenase. This enzymatic oxidation illustrates in situ cofactor regeneration and allows students to measure simple enzyme kinetics.

McClure, Cynthia K.; Chenault, H. Keith

1996-05-01

307

Problem-Based Learning in Undergraduate Instruction. A Sophomore Chemistry Laboratory  

NASA Astrophysics Data System (ADS)

Problem-based learning (PBL) is a pedagogical approach based on recent advances in cognitive science research on human learning. PBL has been used in medical schools for many years. This paper describes the application of PBL in undergraduate courses and discusses the specific needs of this environment. Practical information and guidance are given on how to develop a challenging problem, how to ensure coverage of syllabus topics, how to structure the discussions, and how to evaluate the progress of the course. The paper describes an implementation of PBL methodologies in an undergraduate science course. In this course, students work with a voluntary environmental advocacy group, the Upper Chattahoochee RiverKeeper, to monitor the quality of water in the Chattahoochee watershed. Students learn experimental analytical chemistry techniques and problem-solving skills in this context. A PBL classroom is organized around collaborative problem-solving activities, which provide a context for learning and discovery. Students show enthusiasm and motivation to learn, and they achieve a deeper understanding of the material because it is information they have learned in a context of an interesting problem. PBL also provides them with an opportunity to acquire effective problem-solving techniques and to improve their communication skills and their ability to work in cooperative groups.

Ram, Preetha

1999-08-01

308

Work in progress: a TIMS based laboratory for undergraduate probability and random processes  

Microsoft Academic Search

Laboratory experiments for a junior level electrical engineering course in probability and random processes are described. The laboratory platform is centered on the telecommunications instructional modeling systems (TIMS). All of the laboratory protocols are available for download from the first author's Website.

Lance C. Pérez; Jerald L. Varner; Michael F. Anderson

2004-01-01

309

Bringing the Excitement and Motivation of Research to Students; Using Inquiry and Research-Based Learning in a Year-Long Biochemistry Laboratory: Part II--Research-Based Laboratory--A Semester-Long Research Approach Using Malate Dehydrogenase as a Research Model  

ERIC Educational Resources Information Center

|Research-based learning in a teaching environment is an effective way to help bring the excitement and experience of independent bench research to a large number of students. The program described here is the second of a two-semester biochemistry laboratory series. Here, students are empowered to design, execute and analyze their own experiments…

Knutson, Kristopher; Smith, Jennifer; Nichols, Paul; Wallert, Mark A.; Provost, Joseph J.

2010-01-01

310

Innovations in Inquiry-Based Laboratory Exercises for Non-Majors Astronomy Courses: Connecting Undergraduates with the Enterprise of Science  

NASA Astrophysics Data System (ADS)

Connecting Undergraduates to the Enterprise of Science (CUES) is an NSF-funded program which aims to help instructors towards inquiry based laboratory experiences for students. While many science instructors want to move away from the traditional cookbook laboratories, there are many challenges to executing such a change. The CUES program provides an opportunity for instructors to incorporate inquiry, using "minijournals". The basic idea behind the minijournal format is to present students with a simulated journal article about an experiment or investigation. The article outlines the question, methods used, and conclusions and suggests further scientific questions that might be of interest. The students are expected to devise their own experiments or investigations to answer said questions, and to produce their own paper/article describing it. In this way, the students will be engaged in inquiry-based science labs which encompass doing, thinking, and communicating, while the minijournal allows the instructor to contain lines of inquiry within the limits posed by existing resources. Here we present both the rationale for and concept of using minijournals and specific examples of such laboratory exercises we are currently employing in our introductory (non-science majors) astronomy laboratory. In addition, we explore the difficulties implementing such a technique, when the potential astronomy experiments available to students may be limited.

Speck, Angela; Ruzhitskaya, L.; Weaver, J. C.

2009-01-01

311

Zebrafish Development and Genetics: Introducing Undergraduates to Developmental Biology and Genetics in a Large Introductory Laboratory Class  

PubMed Central

Abstract We have taken advantage of the strengths of the zebrafish model system to introduce developmental biology and genetics to undergraduates in their second semester of the Introductory Biology course at Emory. We designed a 6-week laboratory module based on research being undertaken by faculty in the department, and incorporated experiments that used current research methods including bioinformatics. Students undertook a range of experiments including direct observation of live wild-type zebrafish at different stages of embryogenesis, whole-mount in situ hybridization of mutant and wild-type embryos, vital dye staining of mutant and wild-type embryos, and pharmacological treatments to perturb normal development. These laboratories engaged the students by providing a hands-on, research-centered experience, while also enhancing their written (worksheets and laboratory reports) and oral (group presentation) communication skills. We describe the proceedings of each lab and the logistics of preparing and running these labs for 400–500 students (120 students taking lab each day), and provide a preliminary assessment of the success of the laboratories data based on student evaluations.

D'Costa, Allison

2009-01-01

312

Molecular Models for Biochemistry  

NSDL National Science Digital Library

This Carnegie Mellon University website uses Chime and RasMol images to provide students with many biochemistry tutorials and quizzes. Intended as a supplement to a standard biochemistry text, users can find numerous 3-D images of small molecules, amino acids, protein structures, and much more. The website offers extensive viewing suggestions and tutorials on Chime and RasMol. With countless interactive materials and quizzes, biochemistry students can find the help they need at this extraordinary website.

313

Preparation, Purification, and Secondary Structure Determination of Bacillus Circulans Xylanase. A Molecular Laboratory Incorporating Aspects of Molecular Biology, Biochemistry, and Biophysical Chemistry  

ERIC Educational Resources Information Center

|A project module designed for biochemistry or cellular and molecular biology student which involves determining the secondary structure of Bacillus circulans xylanase (BCX) by circular dichroism (CD) spectroscopy under conditions that compromise its stabilizing intramolecular forces is described. The lab model enhanced students knowledge of the…

Russo, Sal; Gentile, Lisa

2006-01-01

314

Preparation of a D-Glucose-Derived Alkene. An E2 Reaction for the Undergraduate Organic Chemistry Laboratory  

NASA Astrophysics Data System (ADS)

As the carbohydrates continue to gain attention for their central role in numerous areas of chemistry and biology, there is a growing need for experiments at the undergraduate level that highlight these fascinating compounds. In this article we describe a simple two-step laboratory experiment that may be used to highlight several important concepts introduced in sophomore organic chemistry, as applied to compounds of potential biological interest. The sequence begins with commercially available "diacetone glucose," which is converted to the highly crystalline tosylate derivative. Upon treatment of compound 2 with potassium tert-butoxide in THF solution, the alkene (3-deoxy-1,2:5,6-di-O-isopropylidene- a- l">D -erythro-hex-3-enofuranose) is formed via an E2 reaction. The synthetic sequence allows for discussion of various concepts within organic chemistry, as well as for the application of analytical techniques such as polarimetry and one- and two-dimensional NMR.

Norris, Peter; Fluxe, Andrew

2001-12-01

315

Undergraduates at Sea and in the Laboratory Conducting Habitat Mapping Using Multibeam and Sidescan Sonar  

NASA Astrophysics Data System (ADS)

During the last five years, undergraduate students at the College of Charleston have had numerous opportunities to take part in the college's Transect Program and sail aboard research vessels on 2-5 day cruises to study the continental shelf. The program's purpose is to train students in oceanographic research while developing a long-term information geodatabase to characterize and monitor essential fish habitats, and to map seafloor geomorphology. During these cruises students take the lead to conduct a variety of research investigations which include hydrographic surveys of the seafloor using sidescan sonar, multibeam bathymetry, and video collected using a remotely operated vehicle and during SCUBA dives. Following the data collection cruises, students have enrolled in semester-long research courses to analyze data and document results through poster and oral presentations. More than 60 students have taken part in at least one of 6 programs. In the past two years, the NOAA Ship NANCY FOSTER has provided invaluable sea time to conduct multibeam surveys of the mid- and outer continental shelf off Charleston, so that the 22 participating Transect students have focused their work on seafloor mapping, and have become trained in state-of-the art CARIS multibeam and sidescan sonar processing software. Most of these students have presented their results at professional meetings, and manuscripts are currently in preparation. Students have had numerous post-program opportunities to conduct further research at sea and in the lab. They have collaborated with NOAA scientists and other investigators, conducting bathymetry data processing and analysis from other regions. Most recently, two program graduates worked with University of Washington investigators to map sites for the Ocean Observatory Initiative Regional Scale Nodes. Several students have been contracted or hired as hydrographic survey technicians, while others have gone to graduate school to continue their work using these invaluable skills learned as undergraduates.

Sautter, L. R.; Harris, M. S.

2008-12-01

316

Parallel Combinatorial Synthesis of Azo Dyes: A Combinatorial Experiment Suitable for Undergraduate Laboratories  

ERIC Educational Resources Information Center

|An experiment in the parallel synthesis of azo dyes that demonstrates the concepts of structure-activity relationships and chemical diversity with vivid colors is described. It is seen that this experiment is suitable for the second-semester organic chemistry laboratory and also for the one-semester organic laboratory.|

Gung, Benjamin W.; Taylor, Richard T.

2004-01-01

317

Synthesis and Biological Testing of Penicillins: An Investigative Approach to the Undergraduate Teaching Laboratory  

ERIC Educational Resources Information Center

The development and implementation of a research-based organic chemistry laboratory experiment is presented. The experiment was designed to simulate a scientific research environment, involve students in critical thinking, and develop the student's ability to analyze and present research-based data. In this experiment, a laboratory class…

Whitaker, Ragnhild D.; Truhlar, Laura M.; Yksel, Deniz; Walt, David R.; Williams, Mark D.

2010-01-01

318

Using Model Organisms in an Undergraduate Laboratory to Link Genotype, Phenotype, and the Environment  

ERIC Educational Resources Information Center

|We developed laboratory exercises using zebrafish ("Danio rerio") and nematodes ("Caenorhabditis elegans") for a sophomore-level Integrative Biology Laboratory course. Students examined live wildtype zebrafish at different stages of development and noted shifts occurring in response to "fgf8a" deficiency. Students were introduced to development…

Jacobs-McDaniels, Nicole L.; Maine, Eleanor M.; Albertson, R. Craig; Wiles, Jason R.

2013-01-01

319

Using Laboratory Experiments and Circuit Simulation IT Tools in an Undergraduate Course in Analog Electronics  

ERIC Educational Resources Information Center

Laboratory-based courses play a significant role in engineering education. Given the role of electronics in engineering and technology, laboratory experiments and circuit simulation IT tools are used in their teaching in several academic institutions. This paper discusses the characteristics and benefits of both methods. The content and structure…

Baltzis, Konstantinos B.; Koukias, Konstantinos D.

2009-01-01

320

Advanced Laboratory at Texas State University: Error Analysis, Experimental Design, and Research Experience for Undergraduates  

Microsoft Academic Search

Physics is an experimental science. In other words, all physical laws are based on experimentally observable phenomena. Therefore, it is important that all physics students have an understanding of the limitations of certain experimental techniques and the associated errors associated with a particular measurement. The students in the Advanced Laboratory class at Texas State perform three detailed laboratory experiments during

Carl Ventrice

2009-01-01

321

An Undergraduate Laboratory Experiment that Utilizes a Glass Fiber Filter Assay to Determine the Steroid Specificity and Equilibrium Binding Properties of Glucocorticoid Receptors.  

ERIC Educational Resources Information Center

|Describes two complementary laboratory exercises that use the glass fiber assay to assess receptor specificity and hormone binding affinity in rat liver cytoplasmic extracts. Details the methods, materials and protocol of the experiments. Discusses the basic concepts illustrated and the feasibility of using the experiments at the undergraduate

John, Nancy J.; Firestone, Gary L.

1987-01-01

322

Microwave-Assisted Synthesis of a Natural Insecticide on Basic Montmorillonite K10 Clay. Green Chemistry in the Undergraduate Organic Laboratory  

ERIC Educational Resources Information Center

|A detailed investigation of the clay-catalyzed condensation of sesamol and other phenols with 3-methyl-2-butenal to give methylenedioxyprecocene (MDP) and other chromenes is presented. The clay-catalyzed microwave-assisted condensation of sesamol with 3-methyl-2-butenal is appropriate for incorporation into undergraduate organic laboratory

Dintzner, Matthew R.; Wucka, Paul R.; Lyons, Thomas W.

2006-01-01

323

Using Mole Ratios of Electrolytic Products of Water for Analysis of Household Vinegar: An Experiment for the Undergraduate Physical Chemistry Laboratory  

ERIC Educational Resources Information Center

|A simple 3-h physical chemistry undergraduate experiment for the quantitative analysis of acetic acid in household vinegar is presented. The laboratory experiment combines titration concept with electrolysis and an application of the gas laws. A vinegar sample was placed in the cathode compartment of the electrolysis cell. Electrolysis of water…

Dabke, Rajeev B.; Gebeyehu, Zewdu

2012-01-01

324

Peer Assessment in Large Undergraduate Classes: An Evaluation of a Procedure for Marking Laboratory Reports and a Review of Related Practices  

ERIC Educational Resources Information Center

|This study provides evidence that peer marking can be a reliable tool for assessing laboratory reports in large cohorts. It was conducted over a 4-yr period with first-year undergraduates ([asymptotically equivalent to]180 students/cohort) taking a mammalian physiology course, but the procedure adopted would be applicable to any other…

Harris, Judy R.

2011-01-01

325

Synthesis and Catalytic Activity of Ruthenium-Indenylidene Complexes for Olefin Metathesis: Microscale Experiments for the Undergraduate Inorganic or Organometallic Laboratories  

ERIC Educational Resources Information Center

|A series of experiments for undergraduate laboratory courses (e.g., inorganic, organometallic or advanced organic) have been developed. These experiments focus on understanding the design and catalytic activity of ruthenium-indenylidene complexes for olefin metathesis. Included in the experiments are the syntheses of two ruthenium-indenylidene…

Pappenfus, Ted M.; Hermanson, David L.; Ekerholm, Daniel P.; Lilliquist, Stacie L.; Mekoli, Megan L.

2007-01-01

326

Synthesis and Characterization of Aldol Condensation Products from Unknown Aldehydes and Ketones: An Inquiry-Based Experiment in the Undergraduate Laboratory  

ERIC Educational Resources Information Center

|An experiment for the undergraduate chemistry laboratory in which students perform the aldol condensation on an unknown aldehyde and an unknown ketone is described. The experiment involves the use of techniques such as TLC, column chromatography, and recrystallization, and compounds are characterized by [to the first power]H NMR, GC-MS, and FTIR.…

Angelo, Nicholas G.; Henchey, Laura K.; Waxman, Adam J.; Canary, James W.; Arora, Paramjit S.; Wink, Donald

2007-01-01

327

An Undergraduate Laboratory Experiment that Utilizes a Glass Fiber Filter Assay to Determine the Steroid Specificity and Equilibrium Binding Properties of Glucocorticoid Receptors.  

ERIC Educational Resources Information Center

Describes two complementary laboratory exercises that use the glass fiber assay to assess receptor specificity and hormone binding affinity in rat liver cytoplasmic extracts. Details the methods, materials and protocol of the experiments. Discusses the basic concepts illustrated and the feasibility of using the experiments at the undergraduate

John, Nancy J.; Firestone, Gary L.

1987-01-01

328

Microwave-Assisted Synthesis of a Natural Insecticide on Basic Montmorillonite K10 Clay. Green Chemistry in the Undergraduate Organic Laboratory  

ERIC Educational Resources Information Center

A detailed investigation of the clay-catalyzed condensation of sesamol and other phenols with 3-methyl-2-butenal to give methylenedioxyprecocene (MDP) and other chromenes is presented. The clay-catalyzed microwave-assisted condensation of sesamol with 3-methyl-2-butenal is appropriate for incorporation into undergraduate organic laboratory

Dintzner, Matthew R.; Wucka, Paul R.; Lyons, Thomas W.

2006-01-01

329

A Western Blot-based Investigation of the Yeast Secretory Pathway Designed for an Intermediate-Level Undergraduate Cell Biology Laboratory  

ERIC Educational Resources Information Center

|The movement of newly synthesized proteins through the endomembrane system of eukaryotic cells, often referred to generally as the secretory pathway, is a topic covered in most intermediate-level undergraduate cell biology courses. An article previously published in this journal described a laboratory exercise in which yeast mutants defective in…

Hood-DeGrenier, Jennifer K.

2008-01-01

330

Implementing POGIL in the Lecture and the Science Writing Heuristic in the Laboratory--Student Perceptions and Performance in Undergraduate Organic Chemistry  

ERIC Educational Resources Information Center

|This study investigated the possible connection between effective laboratory activities and student performance on lecture exams. In a traditional undergraduate organic chemistry course for non-science majors, students could predict the products of organic reactions, but struggled to provide reaction mechanisms for those same reactions, despite…

Schroeder, Jacob D.; Greenbowe, Thomas J.

2008-01-01

331

Implementing POGIL in the Lecture and the Science Writing Heuristic in the Laboratory--Student Perceptions and Performance in Undergraduate Organic Chemistry  

ERIC Educational Resources Information Center

This study investigated the possible connection between effective laboratory activities and student performance on lecture exams. In a traditional undergraduate organic chemistry course for non-science majors, students could predict the products of organic reactions, but struggled to provide reaction mechanisms for those same reactions, despite…

Schroeder, Jacob D.; Greenbowe, Thomas J.

2008-01-01

332

A Solvent-Free Baeyer-Villiger Lactonization for the Undergraduate Organic Laboratory: Synthesis of Gamma-T-Butyl-Epsilon-Caprolactone  

ERIC Educational Resources Information Center

|The solvent-free or solid-state reaction systems like the Baeyer-Villiger rearrangement have become popular in the synthetic organic community and viable option for undergraduate laboratory series to reduce waste and cost and simplify reaction process. The reaction is an efficient method to transform ketones to esters and lactones.|

Esteb, John J.; Hohman, Nathan J.; Schlamandinger, Diana E.; Wilson, Anne M.

2005-01-01

333

Synthesis and Characterization of Aldol Condensation Products from Unknown Aldehydes and Ketones: An Inquiry-Based Experiment in the Undergraduate Laboratory  

ERIC Educational Resources Information Center

An experiment for the undergraduate chemistry laboratory in which students perform the aldol condensation on an unknown aldehyde and an unknown ketone is described. The experiment involves the use of techniques such as TLC, column chromatography, and recrystallization, and compounds are characterized by [to the first power]H NMR, GC-MS, and FTIR.…

Angelo, Nicholas G.; Henchey, Laura K.; Waxman, Adam J.; Canary, James W.; Arora, Paramjit S.; Wink, Donald

2007-01-01

334

A Simplified Undergraduate Laboratory Experiment to Evaluate the Effect of the Ionic Strength on the Equilibrium Concentration Quotient of the Bromcresol Green Dye  

ERIC Educational Resources Information Center

|A modified laboratory experiment for undergraduate students is presented to evaluate the effects of the ionic strength, "I", on the equilibrium concentration quotient, K[subscript c], of the acid-base indicator bromcresol green (BCG). The two-step deprotonation of the acidic form of the dye (sultone form), as it is dissolved in water, yields…

Rodriguez, Hernan B.; Mirenda, Martin

2012-01-01

335

The Use of Contextual Learning to Teach Biochemistry to Dietetic Students  

ERIC Educational Resources Information Center

|This article describes the use of contextualized and "blended" learning to teach biochemistry to dietetic students during the second year of their professional training in a 4-year undergraduate degree (Bachelor of Nutrition and Dietetics). Contextualized content was used to engage students and motivate them to learn biochemistry, which many…

Macaulay, J. O.; Van Damme, M. -P.; Walker, K. Z.

2009-01-01

336

Reproduction, Physiology and Biochemistry  

Technology Transfer Automated Retrieval System (TEKTRAN)

This chapter focuses on the reproduction, physiology, and biochemistry of the root-knot nematodes. The extensive amount of information on the reproduction and cytogenetics of species of Meloidogyne contrasts with the limited information on physiology, biochemistry, and biochemical pathways. In commo...

337

Molecular Models for Biochemistry  

NSDL National Science Digital Library

The linked pages described here have tutorials and quizzes that are based on Chime and RasMol images of the molecules and macromolecules found in biochemistry. They are intended to complement standard biochemistry texts where more explanation is provided, but where interactive 3-D images of the molecules are not available.

338

Undergraduate Research Ethics Cases  

NSDL National Science Digital Library

The three cases on this University of Delaware website explore several ethical issues and situations that undergraduates might encounter as they make the transition from the classroom laboratory to the research laboratory.

Harold B. White III (University of Delaware ;)

2010-06-18

339

Pure Rotational Spectroscopy of Asymmetric Tops in the Undergraduate Classroom or Laboratory  

NASA Astrophysics Data System (ADS)

Due to concerns of complexity, the asymmetric top, for which ? = {(2B - A - C) / (A - C)} ? ± 1, is feared, or at least avoided, by many instructors when explaining the rigid rotor. However, the spectral patterns formed by cold} asymmetric rigid rotors in the centimeter-wave} region of the electromagnetic spectrum can be easily identified. We will present some techniques for spectral analyses that we have successfully employed with undergraduate students who are either ``pre-quantum mechanics" or are currently enrolled in a chemical quantum mechanics class. The activities are simple, requiring the students to first locate repeating patterns and then apply simple algebraic expressions in order to determine all three rotational constants. The method will be illustrated using the spectra of 2,2,3,3-tetrafluoropropyl trifluoroacetate (CF_3C(=O)OCH_2CF_2CHF_2), (E)-1,3,3,3-tetrafluoropropene (CF_3CH=CHF), 1H,1H,2H-perfluorocyclobutane (CF_2CF_2CHFCH_2), and 2H-nonafluorobutane (CF_3CHFCF_2CF_3). The first two of these species have predominantly a-type spectra, the third has a predominantly b-type spectrum, the fourth has a predominantly c-type spectrum.

Minei, A. J.; Cooke, S. A.

2013-06-01

340

Successful implementation of inquiry-based physiology laboratories in undergraduate major and nonmajor courses.  

PubMed

Recent evidence has demonstrated that inquiry-based physiology laboratories improve students' critical- and analytical-thinking skills. We implemented inquiry-based learning into three physiology courses: Comparative Vertebrate Physiology (majors), Human Physiology (majors), and Human Anatomy and Physiology (nonmajors). The aims of our curricular modifications were to improve the teaching of physiological concepts, teach students the scientific approach, and promote creative and critical thinking. We assessed our modifications using formative (laboratory exams, oral presentations, and laboratory reports) and summative evaluations (surveys, laboratory notebook, and an end of semester project). Students appreciated the freedom offered by the new curriculum and the opportunity to engage in the inquiry process. Results from both forms of evaluation showed a marked improvement due to the curricular revisions. Our analyses indicate an increased confidence in students' ability to formulate questions and hypotheses, design experiments, collect and analyze data, and make conclusions. Thus, we have successfully incorporated inquiry-based laboratories in both major and nonmajor courses. PMID:19047505

Casotti, G; Rieser-Danner, L; Knabb, M T

2008-12-01

341

An undergraduate laboratory exercise to study the effect of darkness on plant gene expression using DNA microarray.  

PubMed

DNA microarrays are microscopic arrays on a solid surface, typically a glass slide, on which DNA oligonucleotides are deposited or synthesized in a high-density matrix with a predetermined spatial order. Several types of DNA microarrays have been developed and used for various biological studies. Here, we developed an undergraduate laboratory exercise using an Arabidopsis DNA microarray to study the gene expression of Brassica rapa, Wisconsin Fast Plant. Genes involved in senescence, cell wall loosening/degradation, and sugar transport were the most upregulated, while those involved in photosynthesis, the elimination of reactive oxygen intermediates associated with photooxidative stress and auxin synthesis, were the most downregulated. Students were able to complete the experiment successfully. Throughout the exercise, they learned various important molecular techniques including RNA isolation, quantification, reverse transcription, cRNA synthesis, labeling and purification, and microarray hybridization, washing, scanning, and feature extraction. The exercise can be integrated into a college-level molecular biology laboratory. The procedure used can be adapted to examine other effects on other organisms. PMID:21591140

Chang, Ming-Mei; Briggs, George M

2007-11-01

342

Using Assessment to Improve Learning in the Biochemistry Classroom  

ERIC Educational Resources Information Center

|In recent years, major drivers of undergraduate science education reform including the National Science Foundation (NSF) and the Howard Hughes Medical Institute (HHMI) have called on college and university instructors to take a more scientific approach to their teaching. Although many biochemistry instructors are gaining confidence in using…

Loertscher, Jennifer

2010-01-01

343

The Use of Multiple Tools for Teaching Medical Biochemistry  

ERIC Educational Resources Information Center

|In this work, we describe the use of several strategies employing the philosophies of active learning and problem-based learning (PBL) that may be used to improve the teaching of metabolic biochemistry to medical and nutritional undergraduate students. The main activities are as follows: 1) a seminar/poster system in a mini-congress format (using…

Se, Alexandre B.; Passos, Renato M.; Ono, Andre H.; Hermes-Lima, Marcelo

2008-01-01

344

Least Squares Fitting of Non-Linear Data in the Undergraduate Laboratory  

Microsoft Academic Search

Least squares fitting of experimental data to a theoretical model is the corner stone of data analysis in many chemical laboratory programs. Even when data is to be fit to non-linear equations, students are routinely taught to linearize the data, apply linear least squares fitting to extract the chemically significant information, and to use the correlation coefficient of this fit

Theresa Julia Zielinski; Robert D. Allendoerfer

1997-01-01

345

Microfluidics Meets Dilute Solution Viscometry: An Undergraduate Laboratory to Determine Polymer Molecular Weight Using a Microviscometer  

ERIC Educational Resources Information Center

|This paper describes a student laboratory experiment to determine the molecular weight of a polymer sample by measuring the viscosity of dilute polymer solutions in a PDMS microfluidic viscometer. Sample data are given for aqueous solutions of poly(ethylene oxide) (PEO). A demonstration of shear thinning behavior using the microviscometer is…

Pety, Stephen J.; Lu, Hang; Thio, Yonathan S.

2011-01-01

346

On Turning Stereotaxic Surgical Failures into Positive Learning Experiences in the Undergraduate Physiological Psychology Laboratory.  

ERIC Educational Resources Information Center

|Failure is frequently the end point of laboratory exercises in physiological psychology that involve stereotaxic surgery by inexperienced students. Lab instructors should caution students to pursue the potential location of misplaced brain lesions using a variety of simple behavioral tests and the Thompson stereotaxic atlas. (Author/RM)|

Wellman, Paul J.

1985-01-01

347

To What Extent Does A-Level Physics Prepare Students for Undergraduate Laboratory Work?  

ERIC Educational Resources Information Center

This paper is a summary of a small-scale research project carried out to investigate the transition from A-level to university physics, with a specific focus on practical or laboratory skills. A brief description of the methods used precedes the headline findings of the research. A non-evidential discussion of the possible reasons behind any…

Thompson, Alaric

2012-01-01

348

Successful Implementation of Inquiry-Based Physiology Laboratories in Undergraduate Major and Nonmajor Courses  

ERIC Educational Resources Information Center

|Recent evidence has demonstrated that inquiry-based physiology laboratories improve students' critical- and analytical-thinking skills. We implemented inquiry-based learning into three physiology courses: Comparative Vertebrate Physiology (majors), Human Physiology (majors), and Human Anatomy and Physiology (nonmajors). The aims of our curricular…

Casotti, G.; Rieser-Danner, L.; Knabb, M. T.

2008-01-01

349

Using Osteoclast Differentiation as a Model for Gene Discovery in an Undergraduate Cell Biology Laboratory  

ERIC Educational Resources Information Center

|A key goal of molecular/cell biology/biotechnology is to identify essential genes in virtually every physiological process to uncover basic mechanisms of cell function and to establish potential targets of drug therapy combating human disease. This article describes a semester-long, project-oriented molecular/cellular/biotechnology laboratory

Birnbaum, Mark J.; Picco, Jenna; Clements, Meghan; Witwicka, Hanna; Yang, Meiheng; Hoey, Margaret T.; Odgren, Paul R.

2010-01-01

350

Using the Polymerase Chain Reaction in an Undergraduate Laboratory to Produce "DNA Fingerprints."  

ERIC Educational Resources Information Center

|Presents a laboratory exercise that demonstrates the sensitivity of the Polymerase Chain Reaction as well as its potential application to forensic analysis during a criminal investigation. Can also be used to introduce, review, and integrate population and molecular genetics topics such as genotypes, multiple alleles, allelic and genotypic…

Phelps, Tara L.; And Others

1996-01-01

351

Dehydration of 2-Methyl-1-Cyclohexanol: New Findings from a Popular Undergraduate Laboratory Experiment  

ERIC Educational Resources Information Center

|The mineral acid-catalyzed dehydration of 2-methyl-1-cyclohexanol has been a popular laboratory exercise in second-year organic chemistry for several decades. The dehydration experiment is often performed by organic chemistry students to illustrate Zaitsev's rule. However, sensitive analytical techniques reveal that the results do not entirely…

Friesen, J. Brent; Schretzman, Robert

2011-01-01

352

Metabolic Pathways of Biochemistry  

NSDL National Science Digital Library

George Washington University provides an online reference of the major metabolic pathways for biochemistry students and scientists. Students can learn about carbohydrate, lipid, and energy metabolism with the help of two- and three-dimensional graphics. The website offers representations of generalized organic reactions to illustrate how to convert one functional group to another group. Users can also find a list of over 250 common abbreviations used in biochemistry. Biochemistry students having difficulty conceiving the dimensionality of metabolic pathways will benefit from the materials at this web site.

353

Metabolic Pathways of Biochemistry  

NSDL National Science Digital Library

The University of Nebraska provides an online reference of the major metabolic pathways for biochemistry students and scientists. Students can learn about carbohydrate, lipid, and energy metabolism with the help of two- and three-dimensional graphics. The website offers representations of generalized organic reactions to illustrate how to convert one functional group to another group. Users can also find a list of over 250 common abbreviations used in biochemistry. Biochemistry students having difficulty conceiving the dimensionality of metabolic pathways will benefit from the materials at this web site.

2007-05-12

354

Biochemistry Companion Website  

NSDL National Science Digital Library

Academic publishing company Addison-Wesley provides this online Companion Website for the textbook Biochemistry, by Mathews, van Holde, and Ahern (Third Edition). The Companion Website contains Outlines, Concepts, Terminology, and Quizzing sections intended to complement the textbook and to assist students in the learning process. A major contribution of this Website is its hyperlinked structure, allowing students to navigate easily between chapters, concepts, color images, and concise descriptions. For educators and students of biochemistry alike, this online resource will be a helpful tool for reviewing the important concepts of general biochemistry.

Ahern, Kevin G.; Van Holde, K. E. (Kensal Edward), 1928-; Mathews, Christopher K., 1937-

2000-01-01

355

Differentiation of Capsicum Species via Protein Extraction and SDS-PAGE Analysis: A Two-Day Experiment for Undergraduate Biochemistry Laboratories  

Microsoft Academic Search

The Capsicum genus is comprised of more than 200 varieties of pepper plant that collectively represent only five cultivated spices. As a result of extensive cross-breeding, an 88% similarity in protein coding DNA has developed. While this has made species determination based solely on physical appearance all but impossible, the 12% difference in protein coding DNA has led to subtle

Trey Polvadore; Brittney P. Cooper; Michele R. Harris

2012-01-01

356

Detergent-Based Isolation of Yeast Membrane Rafts: An Inquiry-Based Laboratory Series for the Undergraduate Cell Biology or Biochemistry Lab  

ERIC Educational Resources Information Center

|Lipid rafts have been implicated in numerous cellular processes including cell signaling, endocytosis, and even viral infection. Isolation of these lipid rafts often involves detergent treatment of the membrane to dissolve nonraft components followed by separation of raft regions in a density gradient. We present here an inquiry-based lab series…

Willhite, D. Grant; Wright, Stephen E.

2009-01-01

357

Using Observations to Interpret Magma Processes in the Sierra Nevada: An Undergraduate Petrology Laboratory Exercise  

NASA Astrophysics Data System (ADS)

Undergraduate igneous and metamorphic petrology is often one of the few courses in which students use field, thin section, hand sample and geochemical observations to interpret a suite of related rocks. Many students may not have encountered the idea of separating observation from interpretation prior to petrology; yet being able to distinguish these is an important skill for any budding petrologist to learn. Labs that require students to integrate abstract concepts from the lecture portion of the course to present a coherent story based on observations are essential to producing students that are well versed in petrology. A capstone-type lab allows students use many of their recently acquired skills to solve real problems in petrology. These integrated labs can take a number of forms from a short lab looking at a few related thin sections, to a multi-week lab with specified tasks, to a semester-long project culminating in a paper or a presentation. For the past few years, I have used a suite of rocks from the Sierra Nevada batholith to give petrology students a capstone experience for the igneous portion of the course. Students are given thin sections with hand samples, a map and a table of geochemical analyses and asked to record hand-sample and thin section observations with the idea that these will be used to understand processes that were active during batholith generation. Because students are given geochemical analyses, they are also expected to experiment with the use of graphs (e.g., Harker and spider diagrams) to better understand tables of geochemical analyses. The students use observations about rocks and geochemistry to build a coherent story around these rocks; the final product is a short paper in which they use petrographic observations and geochemical diagrams to back up their interpretations. Although the lab presented is specifically designed around a set of thin sections housed at the University of Wisconsin Oshkosh for an upper level course, the lab is highly adaptable. I present some options for adapting this lab to any set of thin sections and hand samples with associated geochemical analyses. This lab can also be tailored to a variety of skill levels - from 2nd year introductory petrology to a graduate course.

Wenner, J. M.

2003-12-01

358

Students' attitude-related responses to inquiry learning in undergraduate kinesiology laboratory instruction  

NASA Astrophysics Data System (ADS)

The purpose of this investigation was to determine whether the student attitudes are impacted when teaching methods in an undergraduate Kinesiology lab course shift from a traditional, cookbook-style, low inquiry-level to an investigative, high inquiry-level approach. Students participated in five weeks of Level 0-1 (low) inquiry activities, followed by five weeks of a Level 3 (high) inquiry project. The same Likert-scale survey was administered to students before and after each 5-week period. The attitudes measured by the survey included students' (a) attitude to scientific inquiry, (b) adoption of scientific attitudes, (c) enjoyment of science lessons, and (d) motivation in science. Repeated measures ANOVAs revealed no significant change in any of the attitude measures when the survey results from the different time points were compared. An open-ended qualitative survey was given to the students at the end of the semester and provided more insight. When asked to compare the low and high-level inquiry experiences, most students reported enjoying the higher level of inquiry more. On the other hand, most students felt they learned more during the low inquiry-level activities. The reported level of motivation in lab was about the same for both levels. When asked what they liked most about the high-level inquiry project, students favored aspects such as the independence, responsibility, and personal relevance. When asked what they liked the least, most students said there was nothing they disliked. Of the minority of students who did not like the high-level of inquiry, most claimed to be uncomfortable with the lack of structure and guidance. Other findings were that many students expressed a new or increased respect and appreciation for what scientists do. Some students experienced a decrease in their reliance on science to be true and correct. While some students thought the high-level inquiry was harder, others perceived it as being easier. These findings illustrate how students enrolled in the same course can have very different experiences with inquiry. In general, the effect of high-level inquiry was not negative, and in fact, was found to have some desirable effects on students.

Henige, Kimberly Ann

359

Design and evaluation of an undergraduate laboratory course in atmospheric science research  

Microsoft Academic Search

An atmospheric science research laboratory course was created based on the Center for Authentic Science Practice in Education (CASPiE) model. CASPiE was designed to provide first and second-year chemistry students with an opportunity to be an integral part of authentic research projects. The CASPiE model was adapted to atmospheric science and renamed Earth and Atmospheric Science-CASPiE (EAS-CASPiE). Students engaged in

Kathleen M. Quardokus

2010-01-01

360

A Laboratory Information Management System (LIMS) for an Undergraduate Microchip Fabrication Facility  

Microsoft Academic Search

We have built an 1,800 ft2 Class 10,000 cleanroom dedicated to teaching the elements of the microchip fabrication process to a multidisciplinary cohort of students from all areas of engineering, science, and even the humanities. This laboratory, equipped with educational tools that allow the fabrication of silicon enhancement mode and depletion mode MOSFETs at about 100 micron rules on 4-inch

Paul D. Eckerman; Robert W. Hendricks

2001-01-01

361

Advanced Laboratory at Texas State University: Error Analysis, Experimental Design, and Research Experience for Undergraduates  

NASA Astrophysics Data System (ADS)

Physics is an experimental science. In other words, all physical laws are based on experimentally observable phenomena. Therefore, it is important that all physics students have an understanding of the limitations of certain experimental techniques and the associated errors associated with a particular measurement. The students in the Advanced Laboratory class at Texas State perform three detailed laboratory experiments during the semester and give an oral presentation at the end of the semester on a scientific topic of their choosing. The laboratory reports are written in the format of a ``Physical Review'' journal article. The experiments are chosen to give the students a detailed background in error analysis and experimental design. For instance, the first experiment performed in the spring 2009 semester is entitled Measurement of the local acceleration due to gravity in the RFM Technology and Physics Building. The goal of this experiment is to design and construct an instrument that is to be used to measure the local gravitational field in the Physics Building to an accuracy of ±0.005 m/s^2. In addition, at least one of the experiments chosen each semester involves the use of the research facilities within the physics department (e.g., microfabrication clean room, surface science lab, thin films lab, etc.), which gives the students experience working in a research environment.

Ventrice, Carl

2009-04-01

362

Can Random Mutation Mimic Design?: A Guided Inquiry Laboratory for Undergraduate Students  

PubMed Central

Complex biological structures, such as the human eye, have been interpreted as evidence for a creator for over three centuries. This raises the question of whether random mutation can create such adaptations. In this article, we present an inquiry-based laboratory experiment that explores this question using paper airplanes as a model organism. The main task for students in this investigation is to figure out how to simulate paper airplane evolution (including reproduction, inheritance, mutation, and selection). In addition, the lab requires students to practice analytic thinking and to carefully delineate the implications of their results.

Kalinowski, Steven T.; Taper, Mark L.; Metz, Anneke M.

2006-01-01

363

Integrating a Discovery-Based Laboratory to Teach Supply Chain Management Fundamentals in an Undergraduate Management Course  

ERIC Educational Resources Information Center

|Using experiential simulation games is a commonly used pedagogical method to enrich classroom discussions and to facilitate students' learning in supply chain management education at both undergraduate and graduate levels. However, existing games are inappropriate for undergraduate students that are first-time learners of the subject. In this…

Zeng, Amy; Johnson, Sharon

2009-01-01

364

Use of the Herb Gymnema sylvestre to Illustrate the Principles of Gustatory Sensation: An Undergraduate Neuroscience Laboratory Exercise.  

PubMed

The Indian herb Gymnema sylvestre has been used in traditional Ayurvedic medicine for 2000 years, most recently for the treatment of diabetes. Loose leaf Gymnema sylvestre can be prepared as a tea and will impair the ability to taste sugar by blocking sweet receptors on the tongue. This report describes a laboratory exercise easily applied to an undergraduate neuroscience course that can be used to illustrate the principles of gustatory sensation. Combined with a preceding lecture on the primary taste sensations, students experience and appreciate how the primary tastes are combined to produce overall taste. In addition, the exercises outlined here expand upon previously published demonstrations employing Gymnema sylvestre to include illustrations of the different sensory transduction mechanisms associated with each of the four or five primary taste modalities. Students compare their qualitative primary taste experiences to salt, sugar, aspartame, chocolate, and sweet-sour candy prior to and following exposure to Gymnema sylvestre. The herb's impairment of sweet sensation is profound and dramatically alters the perception of sweetness in sugar, chocolate, and candy without altering the perception of the other primary tastes. The exercise has an indelible effect on students because the herb's intense effect compels students to rely on their unique personal experiences to highlight the principles of gustatory sensation. PMID:23493970

Schroeder, Joseph A; Flannery-Schroeder, Ellen

2005-06-15

365

Papers from U.S. Department of Energy Science Undergraduate Laboratory Internship Program (SULI) 2005  

SciTech Connect

Polarization measurements at X-ray and gamma-ray energies can provide crucial information on the emission region around massive compact objects such as black holes and neutron stars. The Polarized Gamma-ray Observer (PoGO) is a new balloon-borne instrument designed to measure polarization from such astrophysical objects in the 30-100 keV range, under development by an international collaboration with members from United States, Japan, Sweden and France. The PoGO instrument has been designed by the collaboration and several versions of prototype models have been built at SLAC. The purpose of this experiment is to test the latest prototype model with a radioactive gamma-ray source. For this, we have to polarize gamma-rays in a laboratory environment. Unpolarized gamma-rays from Am241 (59.5 keV) were Compton scattered at around 90 degrees for this purpose. Computer simulation of the scattering process in the setup predicts a 86% polarization. The polarized beam was then used to irradiate the prototype PoGO detector. The data taken in this experiment showed a clear polarization signal, with a measured azimuthal modulation factor of 0.35 {+-} 0.02. The measured modulation is in very close agreement with the value expected from a previous beam test study of a polarized gamma-ray beam at the Argonne National Laboratories Advanced Photon Source. This experiment has demonstrated that the PoGO instrument (or any other polarimeter in the energy range) can be tested in a libratory with a simple setup to a similar accuracy.

Quinn, Helen, (ed.); /SLAC

2005-12-16

366

Controlled\\/Living Radical Polymerization in the Undergraduate Laboratories. 1. Using ATRP to Prepare Block and Statistical Copolymers of n-Butyl Acrylate and Styrene  

Microsoft Academic Search

A simple method of preparing well-defined (co)polymers has been developed for application in an advanced undergraduate laboratory. The method utilizes atom transfer radical polymerization (ATRP), a controlled\\/living radical polymerization, to prepare difunctional poly(n-butyl acrylate) with bromine end groups, which is chain-extended with styrene to yield an ABA triblock copolymer. Simultaneously, a statistical copolymer of the two monomers is prepared for

Kathryn L. Beers; Brian Woodworth; Krzysztof Matyjaszewski

2001-01-01

367

Two Successful Outreach Programs at Storm Peak Laboratory: GRASP for Undergraduates and Partnership for 5th Grade Science Education  

NASA Astrophysics Data System (ADS)

The Desert Research Institute operates a high elevation facility, Storm Peak Laboratory (SPL), located on the Steamboat Springs Ski Resort at an elevation 10,500 ft. SPL provides an ideal location for long-term atmospheric research. The SPL mission statement is to ensure that the laboratory will continue to integrate climate research and education by advancing discovery and understanding within the field of pollution, aerosol and cloud interactions. During the last year, SPL has created two successful outreach programs reaching very different audiences. First, to engage students from local elementary schools, SPL established a 5th grade climate education program. This program is based on a partnership between SPL and Yampatika's&penvironmental educators. Yampatika is a non-profit outdoor environmental education organization. The program spans three days for each school and includes five elementary schools. During the first day, educators from Yampatika visit each classroom to introduce the concepts of climate and weather as well as teach students how to use scientific equipment. During the field program on the second day, students measure and record information about temperature, pressure, relative humidity, wind speed, and particle concentration while they travel to SPL via the gondola (in winter) or Suburban (in fall). Once at the laboratory, students tour the facility, discuss SPL research activities, and explore application of these activities to their curriculum. Following the field trip, Yampatika educators and SPL scientists will visit the school for a follow-up to help children explore concepts, answer questions, and evaluate students" learning. The second program, Geoscience Research at Storm Peak (GRASP), was designed to engage students from underrepresented groups and created a partnership between three Minority Serving Institutions and the University of Nevada, Reno (UNR). Undergraduate students from Tennessee State University, Howard University, Colorado State at Pueblo, and UNR will gather at SPL in the summer of 2008, via funding from the National Science Foundation. Throughout the year-long GRASP program, students encounter the scientific process - from creating a hypothesis, collecting and analyzing data, and presenting their results. Beginning in April, GRASP students will be presented with a short course in atmospheric science at their local institutions. The students will then begin formulating research questions for their time at SPL. In June, students will travel to SPL and work in small groups measuring cloud properties and trace gases with the guidance of an individual instructor. Data collected will be the basis for continuing student research projects. GRASP students and mentors will communicate regarding the progress of student research projects during the fall semester. All GRASP participants will meet at the campus of Howard University the following winter for a reunion workshop.

Hallar, A. G.; McCubbin, I. B.; Wright, J.

2007-12-01

368

Interactive Concepts in Biochemistry  

NSDL National Science Digital Library

Interactive Concepts in Biochemistry is a nicely designed companion website to Dr. Rodney Boyer's _Concepts in Biochemistry, second edition_. Developed collaboratively by Science Technologies and others, this multimedia site is archived on the Wiley Publishers website. Site visitors will enjoy exploring the engaging and instructive collection of Interactive Animations. The collection includes Animations about DNA Replication, Signal Transduction, Photosynthesis, Cell Structure, Protein Synthesis, and more. The site also features a number of tutorials regarding Kinesin, Myoglobin & Hemoglobin, tRNA, and Protein-DNA Interactions, to name a few. In addition, the website offers reviews of such concepts as Logarithms, Thermodynamics, and Elementary Kinetics; quizzes that correspond to chapters in Boyer's book; and a collection of articles that consider the role of biochemistry in addressing issues like Lactose Intolerance, Alcohol Abuse, and using Methanol as Fuel.

Boyer, Rodney F.

369

Potential alternate life biochemistries  

NASA Astrophysics Data System (ADS)

While life on Earth continues to be discovered in unlikely environments, the underlying biochemistry is all very similar, based on the element carbon, and requiring liquid water. We consider alternate biochemistries based on elements other than carbon, including other group IVA elements, such as silicon and germanium, and solvents other than water. Terminal electron acceptors other than oxygen are also discussed. A fundamental issue is raised related to the detection of, and even the definition of life, whether it is carbon or non-carbon based. An extreme example of this issue would be in consideration of speculative life based on electrically charged dusty plasmas, which may have no physical body.

Konesky, Gregory

2013-09-01

370

University of Arizona: Biochemistry  

NSDL National Science Digital Library

This University of Arizona website provides numerous chemistry tutorials and problem sets primarily for biology students. The lessons are divided into two main categories: chemistry of life and energy reactions. Students can find clear, in-depth explanations of amino acids, pH levels, photosynthesis, metabolism, and more. Each section is filled with helpful, colorful images. The website provides outside resources which cover additional topics in biochemistry. Biochemistry students will surely find these tutorials to be a great addition to their classroom learning experience.

371

Biochemistry Off the Shelf.  

ERIC Educational Resources Information Center

|Provides sources of nonanimal biochemical materials (which are relatively inexpensive, readily available, and require no special storage) suitable for use in biochemistry experiments. They are presented under these headings: (1) enzymes and other proteins; (2) carbohydrates; (3) lipids; (4) nucleic acids; and (5) metabolism. (JN)|

Wilson, Jerry L.

1985-01-01

372

Bringing the Excitement and Motivation of Research to Students; Using Inquiry and Research-Based Learning in a Year-Long Biochemistry Laboratory: Part I--Guided Inquiry--Purification and Characterization of a Fusion Protein--Histidine Tag, Malate Dehydrogenase, and Green Fluorescent Protein  

ERIC Educational Resources Information Center

|A successful laboratory experience provides the foundation for student success, creating active participation in the learning process. Here, we describe a new approach that emphasizes research, inquiry and problem solving in a year-long biochemistry experience. The first semester centers on the purification, characterization, and analysis of a…

Knutson, Kristopher; Smith, Jennifer; Wallert, Mark A.; Provost, Joseph J.

2010-01-01

373

Teaching Undergraduate Research: The One-Room Schoolhouse Model  

ERIC Educational Resources Information Center

|Undergraduate research in the biochemistry, cell, and molecular biology program at Drake University uses apprenticeship, cooperative-style learning, and peer mentoring in a cross-disciplinary and cross-community educational program. We call it the one-room schoolhouse approach to teaching undergraduate research. This approach is cost effective,…

Henderson, LaRhee; Buising, Charisse; Wall, Piper

2008-01-01

374

Preparative Protein Production from Inclusion Bodies and Crystallization: A Seven-Week Biochemistry Sequence  

PubMed Central

We describe how to produce and purify proteins from E. coli inclusion bodies by adapting versatile, preparative-scale techniques to the undergraduate laboratory schedule. This seven-week sequence of experiments fits into an annual cycle of research activity in biochemistry courses. Recombinant proteins are expressed as inclusion bodies, which are collected, washed, then solubilized in urea. Stepwise dialysis to dilute urea over the course of a week produces refolded protein. Column chromatography is used to purify protein into fractions, which are then analyzed with gel electrophoresis and concentration assays. Students culminate the project by designing crystallization trials in sitting-drop trays. Student evaluation of the experience has been positive, listing 5–12 new techniques learned, which are transferrable to graduate research in academia and industry.

Peterson, Megan J.; Snyder, W. Kalani; Westerman, Shelley; McFarland, Benjamin J.

2011-01-01

375

Immersing Undergraduate Students in the Research Experience: A Practical Laboratory Module on Molecular Cloning of Microbial Genes  

ERIC Educational Resources Information Center

|Molecular cloning skills are an essential component of biological research, yet students often do not receive this training during their undergraduate studies. This can be attributed to the complexities of the cloning process, which may require many weeks of progressive design and experimentation. To address this issue, we incorporated an…

Wang, Jack T. H.; Schembri, Mark A.; Ramakrishna, Mathitha; Sagulenko, Evgeny; Fuerst, John A.

2012-01-01

376

The Genomics Education Partnership: Successful Integration of Research into Laboratory Classes at a Diverse Group of Undergraduate Institutions  

ERIC Educational Resources Information Center

Genomics is not only essential for students to understand biology but also provides unprecedented opportunities for undergraduate research. The goal of the Genomics Education Partnership (GEP), a collaboration between a growing number of colleges and universities around the country and the Department of Biology and Genome Center of Washington…

Shaffer, Christopher D.; Alvarez, Consuelo; Bailey, Cheryl; Barnard, Daron; Bhalla, Satish; Chandrasekaran, Chitra; Chandrasekaran, Vidya; Chung, Hui-Min; Dorer, Douglas R.; Du, Chunguang; Eckdahl, Todd T.; Poet, Jeff L.; Frohlich, Donald; Goodman, Anya L.; Gosser, Yuying; Hauser, Charles; Hoopes, Laura L. M.; Johnson, Diana; Jones, Christopher J.; Kaehler, Marian; Kokan, Nighat; Kopp, Olga R.; Kuleck, Gary A.; McNeil, Gerard; Moss, Robert; Myka, Jennifer L.; Nagengast, Alexis; Morris, Robert; Overvoorde, Paul J.; Shoop, Elizabeth; Parrish, Susan; Reed, Kelynne; Regisford, E. Gloria; Revie, Dennis; Rosenwald, Anne G.; Saville, Ken; Schroeder, Stephanie; Shaw, Mary; Skuse, Gary; Smith, Christopher; Smith, Mary; Spana, Eric P.; Spratt, Mary; Stamm, Joyce; Thompson, Jeff S.; Wawersik, Matthew; Wilson, Barbara A.; Youngblom, Jim; Leung, Wilson; Buhler, Jeremy; Mardis, Elaine R.; Lopatto, David; Elgin, Sarah C. R.

2010-01-01

377

The Genomics Education Partnership: Successful Integration of Research into Laboratory Classes at a Diverse Group of Undergraduate Institutions  

ERIC Educational Resources Information Center

|Genomics is not only essential for students to understand biology but also provides unprecedented opportunities for undergraduate research. The goal of the Genomics Education Partnership (GEP), a collaboration between a growing number of colleges and universities around the country and the Department of Biology and Genome Center of Washington…

Shaffer, Christopher D.; Alvarez, Consuelo; Bailey, Cheryl; Barnard, Daron; Bhalla, Satish; Chandrasekaran, Chitra; Chandrasekaran, Vidya; Chung, Hui-Min; Dorer, Douglas R.; Du, Chunguang; Eckdahl, Todd T.; Poet, Jeff L.; Frohlich, Donald; Goodman, Anya L.; Gosser, Yuying; Hauser, Charles; Hoopes, Laura L. M.; Johnson, Diana; Jones, Christopher J.; Kaehler, Marian; Kokan, Nighat; Kopp, Olga R.; Kuleck, Gary A.; McNeil, Gerard; Moss, Robert; Myka, Jennifer L.; Nagengast, Alexis; Morris, Robert; Overvoorde, Paul J.; Shoop, Elizabeth; Parrish, Susan; Reed, Kelynne; Regisford, E. Gloria; Revie, Dennis; Rosenwald, Anne G.; Saville, Ken; Schroeder, Stephanie; Shaw, Mary; Skuse, Gary; Smith, Christopher; Smith, Mary; Spana, Eric P.; Spratt, Mary; Stamm, Joyce; Thompson, Jeff S.; Wawersik, Matthew; Wilson, Barbara A.; Youngblom, Jim; Leung, Wilson; Buhler, Jeremy; Mardis, Elaine R.; Lopatto, David; Elgin, Sarah C. R.

2010-01-01

378

Modeling Stretching Modes of Common Organic Molecules with the Quantum Mechanical Harmonic Oscillator: An Undergraduate Vibrational Spectroscopy Laboratory Exercise  

ERIC Educational Resources Information Center

|An introductory undergraduate physical organic chemistry exercise that introduces the harmonic oscillator's use in vibrational spectroscopy is developed. The analysis and modeling exercise begins with the students calculating the stretching modes of common organic molecules with the help of the quantum mechanical harmonic oscillator (QMHO) model.|

Parnis, J. Mark; Thompson, Matthew G. K.

2004-01-01

379

Curriculum modules, software laboratories, and an inexpensive hardware platform for teaching computational methods to undergraduate computer science students  

Microsoft Academic Search

Computational methods are increasingly important to 21st century research and education; bioinformatics and climate change are just two examples of this trend. In this context computer scientists play an important role, facilitating the development and use of the methods and tools used to support computationally-based approaches. The undergraduate curriculum in computer science is one place where computational tools and methods

Charles Franklin Peck

2009-01-01

380

Using a Molecular-Genetic Approach to Investigate Bacterial Physiology in a Continuous, Research-Based, Semester-Long Laboratory for Undergraduates  

PubMed Central

Designing investigative laboratory exercises that encourage critical thinking, problem solving, and independent thought for upper-division biology courses is a difficult but worthwhile task. In an effort to do so, we developed a semester-long, continuous, research-based investigative laboratory that integrates numerous genetic and molecular biology methods into the investigation of a bacterial physiological process. In this lab, students use random Tn5 transposon mutagenesis to create prodigiosin pigment mutants in the bacterium, Serratia marcescens. This is followed by phenotypic characterization, cloning, and sequencing the Tn insertion site to identify genes involved in pigment biosynthesis. During this lab, students gain ample experience performing basic lab techniques while learning about — and applying — methods for elucidating gene function. The approach to the laboratory and the outcomes are intimately integrated into the teaching of many fundamental physiological processes underlying prodigiosin production in bacteria. The result is a cohesive course that integrates the theory and application of molecular genetic techniques with the study of bacterial physiology. Assessments of student learning objectives demonstrated that students greatly improved their understanding of both physiological processes and the genetic techniques used to investigate them. In addition, students felt that this semester-long exercise provided the necessary laboratory experience they needed and desired in preparation for careers in molecular biology, microbiology, and biochemistry.

Ault, Jeremiah Foster; Renfro, Betsey Marie; White, Andrea Kirsten

2011-01-01

381

Biochemistry (by Jochanan Stenesh)  

NASA Astrophysics Data System (ADS)

Plenum: New York, 1998. Hardcover, ISBN 0 306-45732-6. 95. Paperback, ISBN 0 306 45733 4. 55 (set of 3). Solutions manual and transparencies available. According to the promotional materials accompanying this text, its intended audience is students in one-semester undergraduate biochemistry courses. At just over 500 pages, the book is shorter than the norm of well over 1000 pages. The challenge, then, is to present the subject in a coherent and compelling fashion while necessarily omitting a large fraction of the material that one normally finds in more inclusive texts. That kind of editing is obviously going to lead to squawking from some quarters, so I should put my prejudices on the table. I teach a one-semester course in biochemical structure, and I have a long-standing interest in using molecular models to explain biochemical behavior, both in research and in teaching. The editing performed by Professor Stenesh is likely to trouble someone with a structural or mechanistic background. Rather than selectively excluding some topics, Stenesh has created a table of contents that looks like it's from a much longer text. The usual chapters on biochemical structure, catalysis, metabolism and molecular genetics are included here. The ax fell elsewhere, and most obvious to my eye are the omissions of structure and chemical mechanism beyond those few chapters that are dedicated to them. A brief presentation on the structure and function of hemoglobin is given in the chapter on proteins, and the catalytic mechanism of chymotrypsin is briefly presented in the chapter on catalysis. But in chapters on metabolism, the structures of substrates and products are shown while mechanisms of conversion are omitted. For example, in the description of aldolase, we're informed that the enzyme catalyzes a reverse aldol condensation, but the reader isn't shown how the aldol condensation relates to the chemical conversion we see in the figure. (Part of the problem may be that the text assumes only one semester of preparation in organic chemistry, which might not be adequate for a discussion of biochemical mechanism.) In the section on gene regulation, no mention is made of the atomic-level interactions between regulatory proteins and DNA that lead to specific, high-affinity binding. The helix-turn-helix motif isn't even mentioned. Obviously, in a 500-page text, something had to go. It's too bad, though, that the omitted material includes the structural and mechanistic explanations for the chemical transformations and cellular processes being described. These complaints shouldn't overshadow the fact that this is a thoughtfully constructed text. The writing is both clear and simple. Broken into subsections, topics are presented in brief synopses that carefully identify key terms and ideas, and the problems at the back of the chapters are plentiful and appropriate. Stenesh's presentation is logically sound. I appreciated the presentation of thermodynamics in a chapter that directly precedes metabolism, rather than the more common brief review that appears in the earliest chapters. Likewise, the inclusion of enzyme catalysis directly after protein structure works in the context of this text. I noted a few small errors, but nothing that would cause distractions for the student. Unfortunately, the two-color graphics that accompany the text are less than compelling by current standards (also, a relatively greater number of errors appear in the figures). In drawing chemical structures, little effort is made to show molecular shape. For example, the figures use Fischer projections (without definition before Chapter 5) to define stereoisomers, and only in the appendix are dashes and wedges used to define them. This is particularly problematic in the chapter on amino acids, where their chirality is described but never illustrated. It is difficult to see the three-dimensional concepts being presented in the two-dimensional format being used. In topics where good visuals would be most useful (such as protein and DNA structure), the figures are reminiscent o

Glasfeld, Arthur

1999-06-01

382

Bioinformatics in Undergraduate Education: Practical Examples  

ERIC Educational Resources Information Center

Bioinformatics has emerged as an important research tool in recent years. The ability to mine large databases for relevant information has become increasingly central to many different aspects of biochemistry and molecular biology. It is important that undergraduates be introduced to the available information and methodologies. We present a…

Boyle, John A.

2004-01-01

383

Bioinformatics in Undergraduate Education: Practical Examples  

ERIC Educational Resources Information Center

|Bioinformatics has emerged as an important research tool in recent years. The ability to mine large databases for relevant information has become increasingly central to many different aspects of biochemistry and molecular biology. It is important that undergraduates be introduced to the available information and methodologies. We present a…

Boyle, John A.

2004-01-01

384

Becoming a scientist: A qualitative study of the educational experience of undergraduates working in an American and a Brazilian research laboratory  

NASA Astrophysics Data System (ADS)

Because the production of scientific and technological innovations has been at the center of debates for economic growth, scientists are recognized as important actors in the current global market. In this study, I will examine the undergraduate education of future scientists by focusing on students working in research projects of faculty members. This research activity has been promoted by American and Brazilian public agencies as an attempt to attract more college students to scientific careers as well as to improve their future performance in science. Evaluations of these programs have focused on important quantitative indicators focusing mainly on the amount of students that later choose to pursue scientific careers. However, these studies fail to address important educational aspects of undergraduates' experience. In this research, I explore the educational processes taking place as students are introduced to the making of science in order to understand how and what they are learning. Three bodies of literature illuminates the formulation and the analysis of the research questions: (1) theories of globalization situate the education of scientists within the dynamics of a broader social, economic, cultural, and historical framework; (2) the critical pedagogy of Paulo Freire is the basis for the understanding of the pedagogical processes shaping undergraduate students' experiences within the research site; (3) Critical and Cultural Studies of Science and Technology illuminate the analysis of the complex interactions and practices constructed within the laboratory. In order to understand the educational processes shaping the experiences of undergraduate students engaged in research activities, I conducted a qualitative investigation based on participant-observation and in-depth interviews in an American and a Brazilian laboratories. The two sites constituted insightful case studies that illuminated the understanding of inquires about the training of students in science. In addition, the study of two countries enriched the research inquiry, adding to the findings reflections on the ways differences in national contexts affects scientific training and scientific practices. Mainly, this qualitative research of students in laboratories offers some concrete recommendations and illuminating reflections for science educators, science policy makers, and for those working in the understanding of science epistemologies.

Pascoa, Maria Beatriz Amorim

385

Communicating the Importance of Undergraduate Research to Legislators  

Microsoft Academic Search

The Council on Undergraduate Research provides a unique opportunity for undergraduates to have a positive impact on the future of federal funding for undergraduate research. An annual poster session on Capitol Hill showcases the research of undergraduates conducted at public and private predominantly undergraduate institutions, research universities, and at national laboratories and facilities. This event has generated interest in conducting

Kerry Karukstis

2005-01-01

386

Monitoring in Clinical Biochemistry  

PubMed Central

Monitoring tests form an increasing proportion of the workload in clinical biochemistry and biochemists can help by providing clinicians with information about the variability and precision of tests, the time frame for pharmacodynamic stabilisation after a treatment change, and the frequency of testing. This paper outlines the phases of monitoring, and how to decide if monitoring is beneficial, which test to use for monitoring, when a change in the test result indicates a need for the change in treatment and the length of testing intervals. We conclude with some recommendations for biochemists for future areas of research and advice that can be given to clinicians.

Doust, Jenny; Glasziou, Paul

2013-01-01

387

Clays as mineral dust aerosol: An integrated approach to studying climate, atmospheric chemistry, and biogeochemical effects of atmospheric clay minerals in an undergraduate research laboratory  

NASA Astrophysics Data System (ADS)

Entrained mineral dust aerosol accounts for 45% of the global annual atmospheric aerosol load and can have a significant influence on important environmental issues, including climate, atmospheric chemistry, cloud formation, biogeochemical processes, visibility, and human health. 70% of all mineral aerosol mass originating from Africa consists of layered aluminosilicates, including illite, kaolinite, and montmorillonite clays. Clay minerals are a largely neglected component of mineral aerosol, yet they have unique physiochemical properties, including a high reactive surface area, large cation exchange capacities, small particle sizes, and a relatively large capacity to take up adsorbed water, resulting in expansion of clay layers (and a larger reactive surface area for heterogeneous interactions) in some cases. An integrated laboratory research approach has been implemented at Hendrix College, a Primarily Undergraduate Institution, in which undergraduate students are involved in independent and interdisciplinary research projects that relate the chemical aging processes (heterogeneous chemistry) of clay minerals as a major component of mineral aerosol to their effects on climate (water adsorption), atmospheric chemistry (trace gas uptake), and biogeochemistry (iron dissolution and phytoplankton biomarker studies). Preliminary results and future directions will be reported.

Hatch, C. D.; Crane, C. C.; Harris, K. J.; Thompson, C. E.; Miles, M. K.; Weingold, R. M.; Bucuti, T.

2011-12-01

388

Lasers in the Undergraduate Curriculum II: Coursework Experiments and Research Projects.  

ERIC Educational Resources Information Center

Described are 14 experiments that use lasers. Topic areas include physical chemistry, analytical chemistry, organic chemistry, inorganic chemistry, and biochemistry. Instrumentation and the use of the laser in undergraduate research are discussed. (CW)

Steehler, Jack K.

1990-01-01

389

Lasers in the Undergraduate Curriculum II: Coursework Experiments and Research Projects.  

ERIC Educational Resources Information Center

|Described are 14 experiments that use lasers. Topic areas include physical chemistry, analytical chemistry, organic chemistry, inorganic chemistry, and biochemistry. Instrumentation and the use of the laser in undergraduate research are discussed. (CW)|

Steehler, Jack K.

1990-01-01

390

BIOCHEMISTRY OF SPORULATION I.  

PubMed Central

Hanson, Richard S. (University of Illinois, Urbana), V. R. Srinivasan, and H. Orin Halvorson. Biochemistry of sporulation. I. Metabolism of acetate by vegetative and sporulating cells. J. Bacteriol. 85:451–460. 1963.—The transition from the vegetative to the sporulating cycle in a sporeformer is marked by a change in the enzymatic machinery of the cell. When vegetative cells of Bacillus cereus strain T are grown in a glucose-yeast extract-minerals medium, acetate accumulates until the beginning of the sporulation cycle. The acetate-activating systems are present in the vegetative cells as well as in the cells of the early stages of sporulation, whereas the enzymes necessary for the terminal oxidation of acetate to carbon dioxide are absent in the vegetative stage. The induction of a functional tricarboxylic acid cycle during early sporulation is inhibited by chloramphenicol. ?-Picolinic acid also prevents morphological, as well as physiological, changes during the transition.

Hanson, Richard S.; Srinivasan, V. R.; Halvorson, H. Orin

1963-01-01

391

Design and operation of an inexpensive far-field laser scanning microscope suitable for use in an undergraduate laboratory course  

NASA Astrophysics Data System (ADS)

Scanning microscope applications span the science disciplines yet their costs limit their use at educational institutions. The basic concepts of scanning microscopy are simple. The microscope probe - whether it produces a photon, electron or ion beam - moves relative to the surface of the sample object. The beam interacts with the sample to produce a detected signal that depends on the desired property to be measured at the probe location on the sample. The microscope transforms the signal for output in a form desired by the user. Undergraduate students can easily construct a far-field laser scanning microscope that illustrates each of these principles from parts available at local electronics and hardware stores and use the microscope to explore properties of devices such as light dependent resistors and biological samples such as leaves. Students can record, analyze and interpret results using a computer and free software.

Pallone, Arthur; Hawk, Eric

2012-02-01

392

Teaching of Biochemistry in Medical School: A Well-Trodden Pathway?  

ERIC Educational Resources Information Center

|Biochemistry and molecular biology occupy a unique place in the medical school curriculum. They are frequently studied prior to medical school and are fundamental to the teaching of biomedical sciences in undergraduate medical education. These two circumstances, and the trend toward increased integration among the disciplines, have led to…

Mathews, Michael B.; Stagnaro-Green, Alex

2008-01-01

393

Periodate Titration of Fe(II) in Acid Aqueous Solutions: An Environmentally Friendly Redox Reaction for the Undergraduate Laboratory  

Microsoft Academic Search

An environmentally friendly redox laboratory for the determination of Fe(II) in an acid aqueous medium is presented. This laboratory exercise is an appropriate substitute for the traditional dichromate titration, which is environmentally problematic. This titration method uses the periodate ion as the oxidizing agent and yields results which are as accurate as the dichromate titration. Student success rate in quantitative

THOMAS G. DRUMMOND; WILLIAM L. LOCKHART; SPENCER J. SLATTERY; FAROOQ A. KHAN; ANDREW J. LEAVITT

1997-01-01

394

A Multistep Organocatalysis Experiment for the Undergraduate Organic Laboratory: An Enantioselective Aldol Reaction Catalyzed by Methyl Prolinamide  

ERIC Educational Resources Information Center

|In recent years, there has been an explosion of research concerning the area of organocatalysis. A multistep capstone laboratory project that combines traditional reactions frequently found in organic laboratory curriculums with this new field of research is described. In this experiment, the students synthesize a prolinamide-based organocatalyst…

Wade, Edmir O.; Walsh, Kenneth E.

2011-01-01

395

Controlled\\/Living Radical Polymerization in the Undergraduate Laboratories. 2. Using ATRP in Limited Amounts of Air to Prepare Block and Statistical Copolymers of n-Butyl Acrylate and Styrene  

Microsoft Academic Search

Developments in controlled radical polymerization have facilitated the use of living polymer chemistry in the undergraduate laboratories. In the first paper of this series, a procedure for the use of atom transfer radical polymerization (ATRP) to prepare block and statistical copolymers was described and the use of kinetic analysis to differentiate between living and conventional processes was demonstrated. In this

Krzysztof Matyjaszewski; Kathryn L. Beers; Brian Woodworth; Zachary Metzner

2001-01-01

396

Consortium for Undergraduate Research Experience  

NASA Website

[Students Higher Education] [Available: CA] CURE is a year-round research internship project for underrepresented minority and women undergraduates in the Los Angeles area. CURE matches students with mentors at the Jet Propulsion Laboratory and ...

397

Undergraduate Research  

Microsoft Academic Search

The undergraduate social work curriculum with its requirement of research content is well-positioned to enhance student capacity and practice effectiveness. Research content should move far beyond the conventional thinking as necessary but not critically important at the undergraduate level, to discussions of knowledge enhancement for practice and preparation for advanced education. This article suggests the directions for further thought and

Julia M. Watkins

2007-01-01

398

Microarrays for Undergraduate Classes  

ERIC Educational Resources Information Center

|A microarray experiment is presented that, in six laboratory sessions, takes undergraduate students from the tissue sample right through to data analysis. The model chosen, the murine erythroleukemia cell line, can be easily cultured in sufficient quantities for class use. Large changes in gene expression can be induced in these cells by…

Hancock, Dale; Nguyen, Lisa L.; Denyer, Gareth S.; Johnston, Jill M.

2006-01-01

399

Undergraduate Mass Spectrometer  

Microsoft Academic Search

Described here is a simple static-field mass spectrometer which has had usefulness both as an undergraduate laboratory instrument and as a piece of demonstration apparatus. The design provides for the adjustment of such parameters as magnet position, slit widths and heights, and allows the use of different types of sources and detectors. Ion currents are large enough to be easily

John W. Dewdney

1960-01-01

400

Microarrays for Undergraduate Classes  

ERIC Educational Resources Information Center

A microarray experiment is presented that, in six laboratory sessions, takes undergraduate students from the tissue sample right through to data analysis. The model chosen, the murine erythroleukemia cell line, can be easily cultured in sufficient quantities for class use. Large changes in gene expression can be induced in these cells by…

Hancock, Dale; Nguyen, Lisa L.; Denyer, Gareth S.; Johnston, Jill M.

2006-01-01

401

Using Student-Generated UV-Induced Escherichia coli Mutants in a Directed Inquiry Undergraduate Genetics Laboratory  

PubMed Central

We report a thematic sequence of directed inquiry-based labs taking students from bacterial mutagenesis and phenotypic identification of their own self-created mutant, through identification of mutated genes by biochemical testing, to verification of mutant alleles by complementation, and finally to mutant allele characterization by DNA sequence analysis. The lab utilizes UV mutagenesis with wild-type Escherichia coli and a UV-sensitive isogenic derivative optimized for undergraduate use. The labs take advantage of the simplicity of E. coli in a realistic genetic investigation using safe UV irradiation methods for creation and characterization of novel mutants. Assessment data collected over three offerings of the course suggest that the labs, which combine original investigation in a scientifically realistic intellectual environment with learned techniques and concepts, were instrumental in improving students' learning in a number of areas. These include the development of critical thinking skills and understanding of concepts and methods. Student responses also suggest the labs were helpful in improving students' understanding of the scientific process as a rational series of experimental investigations and awareness of the interdisciplinary nature of scientific inquiry.

Healy, Frank G.; Livingstone, Kevin D.

2010-01-01

402

Update: Biochemistry of Genetic Manipulation.  

ERIC Educational Resources Information Center

|Various topics on the biochemistry of genetic manipulation are discussed. These include genetic transformation and DNA; genetic expression; DNA replication, repair, and mutation; technology of genetic manipulation; and applications of genetic manipulation. Other techniques employed are also considered. (JN)|

Barker, G. R.

1983-01-01

403

Teaching Biochemistry: A Topical Approach.  

ERIC Educational Resources Information Center

Uses the subject of enzymes to illustrate the topical approach to teaching biochemistry at the secondary level. Includes the procedures for three experiments dealing with various aspects of enzymes. (JN)

Maier, Mary L.

1986-01-01

404

Cat dissection vs. sculpting human structures in clay: an analysis of two approaches to undergraduate human anatomy laboratory education.  

PubMed

Many human anatomy courses are taught using cat dissection. Alternatives are available, but information regarding learning outcomes is incomplete. In 2003, approximately 120 undergraduates enrolled in a human anatomy course were assigned to one of two treatment groups. In the control group, students performed cat dissections (emphasizing isolation and identification) of the muscular, digestive, and cardiovascular systems. In the experimental treatment group, students built clay sculptures of each human body system. Student learning was evaluated by using both low- and high-difficulty questions. On pre- and postexperiment control exams, there were no significant differences in student performance. On exams after a cat dissection vs. a human-clay sculpting experience, the students in the human-clay sculpting treatment group scored significantly higher than their classmates in the cat dissection group on both the low- and high-difficulty questions. Student attitudes toward dissection and taking future human anatomy courses were also measured. There were no differences in student attitudes at the beginning of the experiment; afterward, students exposed to a cat dissection experience viewed dissection more favorably than students in the human-clay sculpting treatment group. There were no treatment effects on student willingness to take future human anatomy courses. The experimental design makes it difficult to conclude precisely why students assigned to the human-clay sculpting experience performed better on exams, but as each method was performed in this particular human anatomy course, our data indicate that human-clay sculpting may be a viable alternative to cat dissection in an anatomy course in which the students focus on human anatomy. PMID:15718380

Waters, John R; Van Meter, Peggy; Perrotti, William; Drogo, Salvatore; Cyr, Richard J

2005-03-01

405

Synthesis and Multinuclear Lanthanide Shift Reagent NMR Analysis of 1- and 2-Adamantanol: An Advanced Undergraduate Laboratory Project.  

ERIC Educational Resources Information Center

|Reports on a project used in a junior-level laboratory in which students prepare two alcohols, characterize these compounds, and use a shift reagent for structure determination and peak assignment. Background information, materials needed, procedures used, and typical results obtained are included. (JN)|

Schaeffer, Charles D., Jr.; Yoder, Claude H.

1985-01-01

406

Hands-On Experiences of Undergraduate Students in Automatics and Robotics Using a Virtual and Remote Laboratory  

ERIC Educational Resources Information Center

|Automatics and Robotics subjects are always greatly improved when classroom teaching is supported by adequate laboratory courses and experiments following the "learning by doing" paradigm, which provides students a deep understanding of theoretical lessons. However, expensive equipment and limited time prevent teachers having sufficient…

Jara, Carlos A.; Candelas, Francisco A.; Puente, Santiago T.; Torres, Fernando

2011-01-01

407

A Simple LIBS (Laser-Induced Breakdown Spectroscopy) Laboratory Experiment to Introduce Undergraduates to Calibration Functions and Atomic Spectroscopy  

ERIC Educational Resources Information Center

|This laboratory experiment introduces students to a different type of atomic spectroscopy: laser-induced breakdown spectroscopy (LIBS). LIBS uses a laser-generated spark to excite the sample; once excited, the elemental emission is spectrally resolved and detected. The students use LIBS to analyze a series of standard synthetic silicate samples…

Chinni, Rosemarie C.

2012-01-01

408

Synthesis and Multinuclear Lanthanide Shift Reagent NMR Analysis of 1- and 2-Adamantanol: An Advanced Undergraduate Laboratory Project.  

ERIC Educational Resources Information Center

Reports on a project used in a junior-level laboratory in which students prepare two alcohols, characterize these compounds, and use a shift reagent for structure determination and peak assignment. Background information, materials needed, procedures used, and typical results obtained are included. (JN)

Schaeffer, Charles D., Jr.; Yoder, Claude H.

1985-01-01

409

Determination of Spin-Lattice Relaxation of Time Using (Super 13)C NMR: An Undergraduate Physical Chemistry Laboratory Experiment  

ERIC Educational Resources Information Center

An experiment designed for the physical chemistry laboratory where (super 13)C NMR is applied to determine the spin-lattice relaxation time for carbon atoms in n-hexanol is proposed. It is concluded that students learn the principles and concepts of NMR spectroscopy as well as dynamic NMR experiments.

Gasyna, Zbigniew L.; Jurkiewicz, Antoni

2004-01-01

410

Genomics and Bioinformatics in Undergraduate Curricula: Contexts for Hybrid Laboratory/Lecture Courses for Entering and Advanced Science Students  

ERIC Educational Resources Information Center

|Emerging interest in genomics in the scientific community prompted biologists at James Madison University to create two courses at different levels to modernize the biology curriculum. The courses are hybrids of classroom and laboratory experiences. An upper level class uses raw sequence of a genome (plasmid or virus) as the subject on which to…

Temple, Louise; Cresawn, Steven G.; Monroe, Jonathan D.

2010-01-01

411

Plant residue biochemistry regulates soil carbon cycling and carbon sequestration  

Microsoft Academic Search

Substrate composition is one of the most important factors influencing the decomposition of plant residues in soils. The interaction of organic residue biochemistry with residue decomposition rates, soil aggregation and soil humus composition was determined in a laboratory experiment. Addition of seven different organic residues (2% w\\/w alfalfa, oat, canola, clover, soybean, corn and prairie grasses) to a Webster soil

Dean A. Martens

2000-01-01

412

Estimating leaf biochemistry using the PROSPECT leaf optical properties model  

Microsoft Academic Search

The biophysical, biochemical, and optical properties of 63 fresh leaves and 58 dry leaves were measured to investigate the potential of remote sensing to estimate the leaf biochemistry from space. Almost 2000 hemispherical reflectance and transmittance spectra were acquired from 400 nm to 2500 nm using a laboratory spectrophotometer. The amount of chlorophyll, water, protein, cellulose, hemicellulose, lignin, and starch

S. Jacquemoud; S. L. Ustin; J. Verdebout; G. Schmuck; G. Andreoli; B. Hosgood

1996-01-01

413

Undergraduate Syllabi.  

ERIC Educational Resources Information Center

|Presents sample undergraduate syllabi for seven addiction counseling courses. Courses include: Group Interventions in Substance Abuse and Addiction; Recovery and Relapse Prevention Methods; Group Counseling I and II; and Co-Occurring Disorders. (GCP)|

Journal of Teaching in the Addictions, 2003

2003-01-01

414

Foundational concepts and underlying theories for majors in "biochemistry and molecular biology".  

PubMed

Over the past two years, through an NSF RCN UBE grant, the ASBMB has held regional workshops for faculty members and science educators from around the country that focused on identifying: 1) core principles of biochemistry and molecular biology, 2) essential concepts and underlying theories from physics, chemistry, and mathematics, and 3) foundational skills that undergraduate majors in biochemistry and molecular biology must understand to complete their major coursework. Using information gained from these workshops, as well as from the ASBMB accreditation working group and the NSF Vision and Change report, the Core Concepts working group has developed a consensus list of learning outcomes and objectives based on five foundational concepts (evolution, matter and energy transformation, homeostasis, information flow, and macromolecular structure and function) that represent the expected conceptual knowledge base for undergraduate degrees in biochemistry and molecular biology. This consensus will aid biochemistry and molecular biology educators in the development of assessment tools for the new ASBMB recommended curriculum. © 2013 by The International Union of Biochemistry and Molecular Biology, 41(5):289-296, 2013. PMID:24019234

Tansey, John T; Baird, Teaster; Cox, Michael M; Fox, Kristin M; Knight, Jennifer; Sears, Duane; Bell, Ellis

2013-09-10

415

The simulated purification of an enzyme as a 'dry' practical within an introductory course of biochemistry.  

PubMed

This article describes a 'dry-laboratory' practical in which the multi-step purification of an enzyme is simulated. It has been devised to be implemented with beginner undergraduates taking an introductory course in biochemistry, with the aim of giving them a glimpse of the intensity of effort involved in complex, research-oriented experiments. The purification steps simulated are: preparation of a liver soluble extract, ammonium sulfate fractionation, gel filtration, ion-exchange and dye-ligand affinity chromatography. Before the simulation, our students are familiar with the preparative and analytical techniques involved, through short 'cookbook' laboratory experiments and, in some cases, ad hoc demonstrations. For the simulation, the students are given detailed protocols of the preparative and analytical experiments, and the raw numeric or graphical data obtained. They have to perform the calculations and graphing necessary to produce a purification table. The 27-page Student Booklet needed to implement the practical is offered by the authors to interested teachers, as a printable electronic file. PMID:10878311

Cameselle; Cabezas; Canales; Jesús Costas M; Faraldo; Fernández; María Pinto R; Meireles Ribeiro J

2000-05-01

416

Photochemical Preparation and Radioactivity of cis-Cr(CO)4(CH3CN)2: An Advanced Undergraduate Inorganic Laboratory Experiment  

NASA Astrophysics Data System (ADS)

The photochemical synthesis of a disubstituted complex, cis-Cr(CO)4(CH3CN)2, by reaction of the parent metal carbonyl, Cr(CO)6, in acetonitrile solution is reported. Subsequent reaction of this intermediate with mono- and bidentate phosphine ligands, triphenylphosphine (PPh3) and bis(diphenylphosphino)methane, Ph2PCH2PPh2 (dppm), yields the geometric isomers, trans-Cr(CO)4(PPh3)2 and cis-Cr(CO)4(dppm), respectively. The experiment employs laboratory techniques commonly used in organometallic chemistry. Dry solvent purification, vacuum-line techniques, inert atmosphere photochemical methods and infrared spectroscopic analysis are required for the preparation, handling and characterization of these compounds.

McNeese, Timothy J.; Ezbiansky, Karin A.

1996-06-01

417

Linkage Isomerization by Two-Dimensional 31P Nuclear Magnetic Resonance Spectroscopy. An Undergraduate Inorganic Laboratory Experiment  

NASA Astrophysics Data System (ADS)

An application of two-dimensional 31P NMR spectroscopy is demonstrated through the characterization of linkage isomers of tetraammine(triphosphato)cobalt(III) chelates and metal-promoted hydrolysis of inorganic triphosphate ion. Unambiguous assignments of 31P resonances for two isomers, a,g and b,g chelates, were achieved by the direct through-bond connectivities among the three phosphate groups. The lack of such connectivity also helped to identify cobalt(III)-catalyzed hydrolysis products, orthophosphate and pyrophosphate ions. The experiment provides background information on 2-D NMR spectroscopy including the significance of pulse calibration, relaxation, evolution and mixing times, data processing, and finally, hands-on training for performing 2-D NMR experiments. The experiment is ideally suited for a senior laboratory course and may be included in the core inorganic curriculum required for a certified ACS B.S. degree.

Bose, Rathindra N.; Al-Ajlouni, Ahmad M.; Volckova, Erika

2001-01-01

418

The Development of Yugoslav Medical Biochemistry.  

National Technical Information Service (NTIS)

Under present ordinances on specialized training in the health service, there is specialized training in medical biochemistry. Specialized training in medical biochemistry should be extended to physicians who complete the third stage of instruction in med...

I. Ruzdic

1967-01-01

419

Reciprocity within biochemistry and biology service-learning.  

PubMed

Service-learning has become a popular pedagogy because of its numerous and far-reaching benefits (e.g. student interest, engagement, and retention). In part, the benefits are a result of the student learning while providing a service that reflects a true need-not simply an exercise. Although service-learning projects have been developed in the areas of Biochemistry and Biology, many do not require reciprocity between the student and those being served. A reciprocal relationship enables a depth in learning as students synthesize and integrate their knowledge while confronting a real-life need. A novel reciprocal service-learning project within a three-semester undergraduate research course in the areas of Biochemistry and Biology is presented. The goal of the project was agreed upon through joint meetings with the partner institution (The Wilds) to develop an in-house competitive ELISA pregnane diol assay. Student progress and achievements were followed through the use of rubrics and progress-meetings with The Wilds. A portfolio provided a visual of progress as it contained both the written assignments as well as the rubric. The article describes a specific reciprocal biochemistry and biology service-learning project and provides recommendations on how to adapt this service-learning design for use in other research courses. PMID:21567723

Santas, Amy J

2009-05-01

420

A High School Biochemistry Course.  

ERIC Educational Resources Information Center

|Describes an advanced, senior-level biochemistry course designed for students planning to pursue careers in nursing, engineering, and agriculture. The course follows a college-type format to expose students to college level course work. Course description, outline, and supplementary materials are included. (JM)|

Powers, George B.

1984-01-01

421

Essays in biochemistry. Volume 20  

SciTech Connect

This book contains four papers. The titles of the papers are: The Mechanism of Action of Oestrogens; Mucous Glycoproteins: A Gel of a Problem; Actin and Myosin Multigene Families: Their Expression During the Formation of Skeletal Muscle; and The Biochemistry of an Inefficient Tissue: Brown Adipose Tissue.

Campbell, P.W.; Marshall, R.D.

1985-01-01

422

Commentary: Biochemistry Re-Natured  

ERIC Educational Resources Information Center

|In his last commentary on "Biochemistry Denatured," this author dealt with his perception that college students today have spent too little of their childhood years playing outside in nature and as a consequence have not learned basic things about the world from personal experience. This "nature-deficit disorder" removes many opportunities for…

White, Harold B.

2010-01-01

423

Promising New Directions in Biochemistry  

ERIC Educational Resources Information Center

|"Biochemistry," by Lubert Stryer, has become one of the standard textbooks for the field. The Fifth Edition has two new authors: Jeremy Berg, Professor and Director of Biophysics and Biophysical Chemistry at Johns Hopkins University School of Medicine; and John Tymoczko, the Towsley Professor of Biology at Carleton College. The new edition does,…

Olivera, Baldomero M.

2003-01-01

424

The Relevance of Student Seminars on Clinically Related Subjects in a Biochemistry Course for Medical and Nutrition Students  

ERIC Educational Resources Information Center

|The aim of this study was to determine the value of a system of seminars on clinically related biochemistry topics for undergraduate students in medicine and nutrition at the University of Brasilia, Brazil. During the second semester of 1998 (1998-2), the teaching staff decided to establish new and stricter rules for the seminar method and to…

Hermes-Lima, Marcelo; Muniz, Karinne C.; Coutinho, Iracema S.

2002-01-01

425

Gender Performance Differences in Biochemistry  

ERIC Educational Resources Information Center

This study examined the historical performance of students at Michigan State University in a two-part biochemistry series Biochem I (n = 5,900) and Biochem II (n = 5,214) for students enrolled from 1997 to 2009. Multiple linear regressions predicted 54.9-87.5% of the variance in student from Biochem I grade and 53.8-76.1% of the variance in…

Rauschenberger, Matthew M.; Sweeder, Ryan D.

2010-01-01

426

Gender Performance Differences in Biochemistry  

ERIC Educational Resources Information Center

|This study examined the historical performance of students at Michigan State University in a two-part biochemistry series Biochem I (n = 5,900) and Biochem II (n = 5,214) for students enrolled from 1997 to 2009. Multiple linear regressions predicted 54.9-87.5% of the variance in student from Biochem I grade and 53.8-76.1% of the variance in…

Rauschenberger, Matthew M.; Sweeder, Ryan D.

2010-01-01

427

Undergraduate basic science preparation for dental school.  

PubMed

In the Institute of Medicines report Dental Education at the Crossroads, it was suggested that dental schools across the country move toward integrated basic science education for dental and medical students in their curricula. To do so, dental school admission requirements and recommendations must be closely reviewed to ensure that students are adequately prepared for this coursework. The purpose of our study was twofold: 1) to identify student dentists' perceptions of their predental preparation as it relates to course content, and 2) to track student dentists' undergraduate basic science course preparation and relate that to DAT performance, basic science course performance in dental school, and Part I and Part II National Board performance. In the first part of the research, a total of ninety student dentists (forty-five from each class) from the entering classes of 1996 and 1997 were asked to respond to a survey. The survey instrument was distributed to each class of students after each completed the largest basic science class given in their second-year curriculum. The survey investigated the area of undergraduate major, a checklist of courses completed in their undergraduate preparation, the relevance of the undergraduate classes to the block basic science courses, and the strength of requiring or recommending the listed undergraduate courses as a part of admission to dental school. Results of the survey, using frequency analysis, indicate that students felt that the following classes should be required, not recommended, for admission to dental school: Microbiology 70 percent, Biochemistry 54.4 percent, Immunology 57.78 percent, Anatomy 50 percent, Physiology 58.89 percent, and Cell Biology 50 percent. The second part of the research involved anonymously tracking undergraduate basic science preparation of the same students with DAT scores, the grade received in a representative large basic science course, and Part I and Part II National Board performance. Using T-test analysis correlations, results indicate that having completed multiple undergraduate basic science courses (as reported by AADSAS BCP hours) did not significantly (p < .05) enhance student performance in any of these parameters. Based on these results, we conclude that student dentists with undergraduate preparation in science and nonscience majors can successfully negotiate the dental school curriculum, even though the students themselves would increase admission requirements to include more basic science courses than commonly required. Basically, the students' recommendations for required undergraduate basic science courses would replicate the standard basic science coursework found in most dental schools: anatomy, histology, biochemistry, microbiology, physiology, and immunology plus the universal foundation course of biology. PMID:12484677

Humphrey, Sue P; Mathews, Robert E; Kaplan, Alan L; Beeman, Cynthia S

2002-11-01

428

Biochemistry and pharmacology of 7?-substituted androstenediones as aromatase inhibitors  

Microsoft Academic Search

The inhibition of aromatase, the enzyme responsible for converting androgens to estrogens, is therapeutically useful for the endocrine treatment of hormone-dependent breast cancer. Research by our laboratory has focused on developing competitive and irreversible steroidal aromatase inhibitors, with an emphasis on synthesis and biochemistry of 7?-substituted androstenediones. Numerous 7?-thiosubstituted androst-4-ene-3,17-diones are potent competitive inhibitors, and several 1,4-diene analogs, such as

Robert W. Brueggemeier; Jill M. O'Reilly; Carl J. Lovely; Patrick J. Ward; Anne L. Quinn; David Baker; Michael V. Darby; Xin-Ju Gu; Nancy E. Gilbert

1997-01-01

429

Council on Undergraduate Research on the Web  

NSDL National Science Digital Library

The mission of the Council of Undergraduate Research (CUR) is to "support and promote high-quality undergraduate student-faculty collaborative research and scholarship." CUR disseminates some of its work and findings through the Council of Undergraduate Research Quarterly (CURQ) and this site provides access to some of the articles from that publication. Currently, visitors can search past articles back to 2008, and visitors can browse through articles like "Putting Undergraduate Research on the Map for Women," "Peer Mentoring in an Introductory Biology Laboratory," and "Undergraduate Research on Writing: Benefits to Faculty and Curriculum Development." All told, the site provides a cornucopia of articles related to making the undergraduate research experience a bit more meaningful, and educators will find much to enjoy here.

2012-02-06

430

Raman Spectroscopy for the Undergraduate Teaching Laboratory: Quantification of Ethanol Concentration in Consumer Alcoholic Beverages and Qualitative Identification of Marine Diesels Using a Miniature Raman Spectrometer  

Microsoft Academic Search

Raman spectroscopy has steadily gained popularity as a powerful tool in both the analytical lab and the undergraduate classroom. The technique is attractive because it allows for rapid, nondestructive qualitative or quantitative analyses of many analytes with little or no sample preparation requirements. The introduction of less expensive, smaller, and more powerful diode laser excitation sources and the recent availability

Danielle Cleveland; Matthew Carlson; Evan D. Hudspeth; Lauren E. Quattrochi; Kathleen L. Batchler; Shrimati A. Balram; Seongun Hong; Robert G. Michel

2007-01-01

431

An Investigation into the Creation, Stability, and X-Ray Fluorescence Analysis of Early Photographic Processes: An Upper-Level Undergraduate Laboratory  

ERIC Educational Resources Information Center

|Photography is one of the few fine art forms that were initially developed by scientists such as Herschel and Talbot; however, in the modern chemistry curriculum, photography has become divorced from its scientific beginnings and resides in the studio arts department of most universities. An upper-level undergraduate experiment is described in…

Rogge, Corina E.; Bezur, Aniko

2012-01-01

432

Becoming a scientist: A qualitative study of the educational experience of undergraduates working in an American and a Brazilian research laboratory  

Microsoft Academic Search

Because the production of scientific and technological innovations has been at the center of debates for economic growth, scientists are recognized as important actors in the current global market. In this study, I will examine the undergraduate education of future scientists by focusing on students working in research projects of faculty members. This research activity has been promoted by American

Maria Beatriz Amorim Pascoa

2002-01-01

433

An Inquiry-Based Practical for a Large, Foundation-Level Undergraduate Laboratory that Enhances Student Understanding of Basic Cellular Concepts and Scientific Experimental Design  

ERIC Educational Resources Information Center

|Student-centered education involving research experiences or inquiry have been shown to help undergraduate students understand, and become excited about, the process of scientific investigation. These benefits are particularly important for students in the early stages of their degree (Report and Kenny,…

Bugarcic, A.; Zimbardi, K.; Macaranas, J.; Thorn, P.

2012-01-01

434

Teaching Cardiac Electrophysiology Modeling to Undergraduate Students: Laboratory Exercises and GPU Programming for the Study of Arrhythmias and Spiral Wave Dynamics  

ERIC Educational Resources Information Center

As part of a 3-wk intersession workshop funded by a National Science Foundation Expeditions in Computing award, 15 undergraduate students from the City University of New York collaborated on a study aimed at characterizing the voltage dynamics and arrhythmogenic behavior of cardiac cells for a broad range of physiologically relevant conditions…

Bartocci, Ezio; Singh, Rupinder; von Stein, Frederick B.; Amedome, Avessie; Caceres, Alan Joseph J.; Castillo, Juan; Closser, Evan; Deards, Gabriel; Goltsev, Andriy; Ines, Roumwelle Sta.; Isbilir, Cem; Marc, Joan K.; Moore, Diquan; Pardi, Dana; Sadhu, Sandeep; Sanchez, Samuel; Sharma, Pooja; Singh, Anoopa; Rogers, Joshua; Wolinetz, Aron; Grosso-Applewhite, Terri; Zhao, Kai; Filipski, Andrew B.; Gilmour, Robert F., Jr.; Grosu, Radu; Glimm, James; Smolka, Scott A.; Cherry, Elizabeth M.; Clarke, Edmund M.; Griffeth, Nancy; Fenton, Flavio H.

2011-01-01

435

Teaching Cardiac Electrophysiology Modeling to Undergraduate Students: Laboratory Exercises and GPU Programming for the Study of Arrhythmias and Spiral Wave Dynamics  

ERIC Educational Resources Information Center

|As part of a 3-wk intersession workshop funded by a National Science Foundation Expeditions in Computing award, 15 undergraduate students from the City University of New York collaborated on a study aimed at characterizing the voltage dynamics and arrhythmogenic behavior of cardiac cells for a broad range of physiologically relevant conditions…

Bartocci, Ezio; Singh, Rupinder; von Stein, Frederick B.; Amedome, Avessie; Caceres, Alan Joseph J.; Castillo, Juan; Closser, Evan; Deards, Gabriel; Goltsev, Andriy; Ines, Roumwelle Sta.; Isbilir, Cem; Marc, Joan K.; Moore, Diquan; Pardi, Dana; Sadhu, Sandeep; Sanchez, Samuel; Sharma, Pooja; Singh, Anoopa; Rogers, Joshua; Wolinetz, Aron; Grosso-Applewhite, Terri; Zhao, Kai; Filipski, Andrew B.; Gilmour, Robert F., Jr.; Grosu, Radu; Glimm, James; Smolka, Scott A.; Cherry, Elizabeth M.; Clarke, Edmund M.; Griffeth, Nancy; Fenton, Flavio H.

2011-01-01

436

Correlation of Preadmission Organic Chemistry Courses and Academic Performance in Biochemistry at a Midwest Chiropractic Doctoral Program*  

PubMed Central

Purpose: Organic chemistry has been shown to correlate with academic success in the preclinical years of medicine, dentistry, and graduate physiology. The purpose of this study is to examine the relationship between undergraduate organic chemistry grades and first-semester biochemistry grades at a Midwest chiropractic doctoral program. Methods: Students enrolled in a first-semester biochemistry course who had completed the prerequisite courses in organic chemistry offered at this same institution were entered into the study. The total grade for each of the three courses was calculated using the midterm and final exam raw scores with a weighting of 50% each. Analysis consisted of obtaining correlation coefficients between the total grades of organic 1 with biochemistry and organic 2 with biochemistry. Using the biochemistry total grade, the students were divided into quartiles and course grades for both organic chemistry 1 and 2 were calculated. Results: For the 109 students in the study, the correlation coefficient between the biochemistry and organic chemistry 1 and biochemistry and organic chemistry 2 courses was r = 0.744 and r = 0.725, respectively. The difference in organic chemistry grades between those in the first and fourth quartiles was 63.2% and 86.9% for organic chemistry 1 (p < .001) and 60.9% and 79.4% for organic chemistry 2 (p < .001). Conclusion: This study shows that organic chemistry can be used as an indicator of future academic success in a chiropractic biochemistry course. Knowledge of such a relationship could prove useful to identify students who may potentially run into academic difficulty with first-year biochemistry

McRae, Marc P.

2010-01-01

437

Ethanol Metabolism and the Transition from Organic Chemistry to Biochemistry  

NASA Astrophysics Data System (ADS)

To ease the transition from organic chemistry at the beginning of a biochemistry course or at the beginning of the metabolism section of the organic course, an early presentation of the oxidation of ethanol is proposed. Alcohol dehydrogenase and aldehyde dehydrogenase reactions can smooth the introduction to biochemistry, since they involve three of the simplest compounds: ethanol, acetaldehyde, and acetic acid. Using these reactions as a model encourages the study of metabolic pathways by a systematic approach rather than by rote memorization. Reactions that can be presented as variations on a theme include methanol poisoning, the polyol reaction, and, most important, the sequence glycerol-3-phosphate to glyceraldehyde-3-phosphate to 3-phosphoglyceric acid. This last sequence integrates lipid and carbohydrate metabolism and, by comparison with the model reaction, brings out the principles of substrate-level phosphorylation. The method has evoked favorable verbal feedback from students and, in addition to medical and graduate courses, has been successfully used in the biochemical section of an undergraduate organic course.

Feinman, Richard D.

2001-09-01

438

Common student misconceptions in exercise physiology and biochemistry  

NSDL National Science Digital Library

The present study represents a preliminary investigationdesigned to identify common misconceptions in students' understanding of physiological and biochemical topics within the academic domain of sport and exercise sciences. A specifically designed misconception inventory (consisting of 10 multiple-choice questions) was administered to a cohort of level 1, 2, and 3 undergraduate students enrolled in physiology and biochemistry-related modules of the BSc Sport Science degree at the authors' institute. Of the 10 misconceptions proposed by the authors, 9 misconceptions were confirmed. Of these nine misconceptions, only one misconception appeared to have been alleviated by the current teaching strategy employed during the progression from level 1 to 3 study. The remaining eight misconceptions prevailed throughout the course of the degree program, suggesting that students enter and leave university with the same misconceptions in certain areas of exercise physiology and biochemistry. The possible origins of these misconceptions are discussed, as are potential teaching strategies to prevent and/or remediate them for future years.

James P. Morton (Liverpool John Moores University Research Institute for Sport and Exercise Sciences); Dominic A Doran (John Moores University Research Institute for Sport and Exercise Sciences); Don P.M. MacLaren (John Moores University Research Institute for Sport and Exercise Sciences)

2008-01-28

439

Journal of Undergraduate Neuroscience Education  

NSDL National Science Digital Library

This sharp looking website is for the Journal of Undergraduate Neuroscience Education (JUNE) which "publishes peer-reviewed reports of innovations in undergraduate neuroscience education...[and] serves as a mechanism for faculty to exchange information...such as laboratory exercises, new media, curricular considerations, and teaching methods." Visitors will find the "Supplementary Materials" tab near the top of any page to contain movies, lab instructions, papers, and images. Some of the movies include an adult and juvenile "Crayfish Brain Movie," "Larval Dissection Movie" of a drosophila mutant, and "Leg Prep and Spine Stimulation" of a cockroach. The "Previous Issues" tab gives visitors the opportunity to read issues published since 2002. Recent pieces featured in JUNE include "Integrating Community Outreach into the Undergraduate Neuroscience Classroom", "Design Plans for an Inexpensive Tail Flick Analgesia Meter", and "Unquenchable Neuroscience."

2012-01-06

440

Preparation of Buffers. An Experiment for Quantitative Analysis Laboratory  

NASA Astrophysics Data System (ADS)

In our experience, students who have a solid grounding in the theoretical aspects of buffers, buffer preparation, and buffering capacity are often at a loss when required to actually prepare a buffer in a research setting. However, there are very few published laboratory experiments pertaining to buffers. This laboratory experiment for the undergraduate quantitative analysis lab gives students hands-on experience in the preparation of buffers. By preparing a buffer to a randomly chosen pH value and comparing the theoretical pH to the actual pH, students apply their theoretical understanding of the Henderson-Hasselbalch equation, activity coefficients, and the effect of adding acid or base to a buffer. This experiment gives students experience in buffer preparation for research situations and helps them in advanced courses such as biochemistry where a fundamental knowledge of buffer systems is essential.

Buckley, P. T.

2001-10-01

441

In memory of Professor Leonor Michaelis in Nagoya: Great contributions to biochemistry in Japan in the first half of the 20th century.  

PubMed

Leonor Michaelis spent the years of 1922-1926 as Professor of Biochemistry of the Aichi Medical College (now Graduate School of Medicine, Nagoya University) in Nagoya, Japan. Michaelis succeeded in gathering many bright young biochemists from all over Japan into his laboratory, and made tremendous contributions to the promotion of biochemistry in Japan. Michaelis was invited to many places in Japan to present lectures over those years. Kunio Yagi, who was Professor of Biochemistry at Nagoya University in the second half of the 20th century, succeeded in crystallizing the "Michaelis" enzyme-substrate complex. Historically, Michelis has had an enormous impact on biochemistry in Japan. PMID:23628780

Nagatsu, Toshiharu Toshi

2013-04-27

442

Hypothetical Biotechnology Companies: A Role-Playing Student Centered Activity for Undergraduate Science Students  

ERIC Educational Resources Information Center

|Science students leaving undergraduate programs are entering the biotechnology industry where they are presented with issues which require integration of science content. Students find this difficult as through-out their studies, most content is limited to a single subdiscipline (e.g., biochemistry, immunology). In addition, students need…

Chuck, Jo-Anne

2011-01-01

443

Rensselaer Polytechnic Institute: Biochemistry of Metabolism  

NSDL National Science Digital Library

From Rensselaer Polytechnic Institute comes this incredibly comprehensive website of "studio-format" courses devoted to the Biochemistry of Metabolism. For the class, each section is divided into two hour sections which are comprised of lecture, exercises (called studio exercises) and discussion. Developed and maintained by Joyce Diwan, the course is designed for "a classroom with internet-connected computers, or network ports for student laptops. Preferably there should be at least one computer per two students. Equipment for projection from a networked instructor's computer or laptop is essential. A class size of 30-40 students is optimal, although a class with up to 60 students is feasible with competent teaching assistants." The three main topics covered are Molecular Biochemistry I (including Sugars and Polysaccharides, Lipids and Membranes, and may more), Molecular Biochemistry II (including Pentose Phosphate Pathway, the Calvin Cycle, and many more), and Cellular Biochemistry (including Actin cytoskeleton and others).

444

Introducing undergraduate students to science.  

PubMed

Understanding the scientific method fosters the development of critical thinking and logical analysis of information. Additionally, proposing and testing a hypothesis is applicable not only to science, but also to ordinary facts of daily life. Knowing the way science is done and how its results are published is useful for all citizens and mandatory for science students. A 60-h course was created to offer undergraduate students a framework in which to learn the procedures of scientific production and publication. The course's main focus was biochemistry, and it was comprised of two modules. Module I dealt with scientific articles, and Module II with research project writing. Module I covered the topics: 1) the difference between scientific knowledge and common sense, 2) different conceptions of science, 3) scientific methodology, 4) scientific publishing categories, 5) logical principles, 6) deductive and inductive approaches, and 7) critical reading of scientific articles. Module II dealt with 1) selection of an experimental problem for investigation, 2) bibliographic revision, 3) materials and methods, 4) project writing and presentation, 5) funding agencies, and 6) critical analysis of experimental results. The course adopted a collaborative learning strategy, and each topic was studied through activities performed by the students. Qualitative and quantitative course evaluations with Likert questionnaires were carried out at each stage, and the results showed the students' high approval of the course. The staff responsible for course planning and development also evaluated it positively. The Biochemistry Department of the Chemistry Institute of the University of São Paulo has offered the course four times. PMID:21567799

De Ávila, Paulo; Torres, Bayardo B

2010-03-01

445

A New GC-MS Experiment for the Undergraduate Instrumental Analysis Laboratory in Environmental Chemistry: Methyl-t-butyl Ether and Benzene in Gasoline  

NASA Astrophysics Data System (ADS)

With the recent ACS approval of an option in environmental chemistry at the undergraduate level, there is a need for new experiments that illustrate fundamental principles of instrumental analysis in the context of environmental chemistry. We describe an experiment that utilizes combined gas chromatography-mass spectrometry (GC-MS) in the qualitative and quantitative analysis of methyl tert-butyl ether (MTBE) and benzene in gasoline. This is particularly appropriate, given the increased use of oxygenates in reformulated gasolines in the United States. In addition to illustrating the fundamentals of GC and MS, this experiment demonstrates (i) the use of internal standards to improve precision; (ii) the application of the method of standard additions; and (iii) the importance of techniques such as selected ion extraction/monitoring in the identification and measurement of specific highly volatile organic compounds in complex environmental mixtures.

Quach, Dinh T.; Ciszkowski, Nancy A.; Finlayson-Pitts, Barbara J.

1998-12-01

446

An inquiry-based practical for a large, foundation-level undergraduate laboratory that enhances student understanding of basic cellular concepts and scientific experimental design.  

PubMed

Student-centered education involving research experiences or inquiry have been shown to help undergraduate students understand, and become excited about, the process of scientific investigation. These benefits are particularly important for students in the early stages of their degree (Report and Kenny, http://naplesccsunysbedu/Pres/boyernsf/1998). However, embedding such experiences into the curriculum is particularly difficult when dealing with early stage students, who are in larger cohorts and often lack the background content knowledge necessary to engage with primary research literature and research level methods and equipment. We report here the design, delivery, assessment, and subsequent student learning outcomes of a 4-week practical module for 120 students at the beginning of their second year of university, which successfully engages students in designing cell culture experiments and in understanding the molecular processes and machinery involved in the basic cellular process of macropinocytosis. PMID:22615225

Bugarcic, A; Zimbardi, K; Macaranas, J; Thorn, P

2012-02-28

447

Elucidating Bioethics with Undergraduates.  

ERIC Educational Resources Information Center

|Discusses the importance of developing bioethics programs for undergraduate students. Two aspects are considered: (1) current areas of concern and sources of bibliographic information; and (2) problems encountered in undergraduate projects. A list of references is provided. (HM)|

Hoskins, Betty B.; Shannon, Thomas A.

1977-01-01

448

Special-Study Modules in a Problem-Based Learning Medical Curriculum: An Innovative Laboratory Research Practice Supporting Introduction to Research Methodology in the Undergraduate Curriculum  

ERIC Educational Resources Information Center

|We describe the organization of wet-lab special-study modules (SSMs) in the Central Research Laboratory of Dokuz Eylul Medical School, Izmir, Turkey with the aim of discussing the scientific, laboratory, and pedagogical aspects of this educational activity. A general introduction to the planning and functioning of these SSMs is given, along with…

Guner, Gul Akdogan; Cavdar, Zahide; Yener, Nilgun; Kume, Tuncay; Egrilmez, Mehtap Yuksel; Resmi, Halil

2011-01-01

449

What Is Undergraduate Research?  

Microsoft Academic Search

The Council on Undergraduate Research promotes and assists development of collaborative student\\/faculty research at primarily undergraduate colleges and universities. Most science educators today accept such research as a critical component of an undergraduate science education. Research provides the primary opportunity for students to engage in the practice of science. We can draw an analogy between sports training and the education

Judith A. Halstead

1997-01-01

450

The Biochemistry Tetrahedron and the Development of the Taxonomy of Biochemistry External Representations (TOBER)  

ERIC Educational Resources Information Center

|Visual literacy, the ability to interpret and create external representations (ERs), is essential to success in biochemistry. Studies have been conducted that describe students' abilities to use and interpret specific types of ERs. However, a framework for describing ERs derived through a naturalistic inquiry of biochemistry classrooms has not…

Towns, Marcy H.; Raker, Jeffrey R.; Becker, Nicole; Harle, Marissa; Sutcliffe, Jonathan

2012-01-01

451

Physical Models Enhance Molecular Three-Dimensional Literacy in an Introductory Biochemistry Course  

ERIC Educational Resources Information Center

This article reports the results of a recent study to evaluate the usefulness of physical models of molecular structures as a new tool with which to teach concepts of molecular structure and function. Of seven different learning tools used by students in this introductory biochemistry class, the use of the physical models in a laboratory was rated…

Roberts, Jacqueline R.; Hagedorn, Eric; Dillenburg, Paul; Patrick, Michael; Herman, Timothy

2005-01-01

452

Use of Molecular Models for Active Learning in Biochemistry Lecture Courses  

ERIC Educational Resources Information Center

|The pedagogical value of having biochemistry and organic chemistry students build and manipulate physical models of chemical species is well established in the literature. Nevertheless, for the most part, the use of molecular models is generally limited to several laboratory exercises or to demonstrations in the classroom setting. A simple…

Hageman, James H.

2010-01-01

453

Laboratory Techniques for the Blind  

ERIC Educational Resources Information Center

|Describes modifications of laboratory procedures for the BSCS Green Version biology, including dissection, microbiology, animal behavior, physiology, biochemistry, and genetics that make the methods suitable for direct experimentation by blind students. Discusses models as substitutes for microscopy. (AL)|

Tombaugh, Dorothy

1972-01-01

454

The Biochemistry of Anaerobic Digestion.  

National Technical Information Service (NTIS)

The investigation sought to determine by means of radioactive tracer techniques, the biochemical mechanisms in the anaerobic degradation of typical organics found in wastes. Two liter laboratory scale digesters were used in all the radioactive tracer work...

J. S. Jeris Y. Chen T. W. Chi Y. Su C. Weng

1970-01-01

455

The Synthesis and Isolation of N-Tert-Butyl-2-Phenylsuccinamic Acid and N-Tert-Butyl-3-Phenylsuccinamic Acid: An Undergraduate Organic Chemistry Laboratory Experiment  

ERIC Educational Resources Information Center

|The facile, high yielding synthesis of phenylsuccinamic acids is described and one of these syntheses, the reaction of phenylsuccinic anhydride with tert-butylamine, is successfully modified and adapted for use in the second-semester organic chemistry laboratory at St. John's University. Succinamic acids are compounds that contain both the amide…

Cesare, Victor; Sadarangani, Ishwar; Rollins, Janet; Costello, Dennis

2004-01-01

456

Synthesis of a Self-Healing Polymer Based on Reversible Diels-Alder Reaction: An Advanced Undergraduate Laboratory at the Interface of Organic Chemistry and Materials Science  

ERIC Educational Resources Information Center

|This laboratory experiment exposes students to the chemistry of self-healing polymers based on a Diels-Alder reaction. Students accomplish a multistep synthesis of a monomer building block and then polymerize it to form a cross-linked polymer. The healing capability of the polymer is verified by differential scanning calorimetry (DSC)…

Weizman, Haim; Nielsen, Christian; Weizman, Or S.; Nemat-Nasser, Sia

2011-01-01

457

What Is the True Color of Fresh Meat? A Biophysical Undergraduate Laboratory Experiment Investigating the Effects of Ligand Binding on Myoglobin Using Optical, EPR, and NMR Spectroscopy  

ERIC Educational Resources Information Center

With an increased focus on integrated upper-level laboratories, we present an experiment integrating concepts from inorganic, biological, and physical chemistry content areas. Students investigate the effects of ligand strength on the spectroscopic properties of the heme center in myoglobin using UV-vis, [superscript 1]H NMR, and EPR…

Linenberger, Kimberly; Bretz, Stacey Lowery; Crowder, Michael W.; McCarrick, Robert; Lorigan, Gary A.; Tierney, David L.

2011-01-01

458

Introducing Education for Sustainable Development in the Undergraduate Laboratory: Quantitative Analysis of Bioethanol Fuel and Its Blends with Gasoline by Using Solvatochromic Dyes  

ERIC Educational Resources Information Center

|The concept of "Education for Sustainable Development", ESD, has been introduced in a period where chemistry education is undergoing a major change, both in emphasis and methods of teaching. Studying an everyday problem, with an important socio-economic impact in the laboratory is a part of this approach. Presently, the students in many countries…

Galgano, Paula D.; Loffredo, Carina; Sato, Bruno M.; Reichardt, Christian; El Seoud, Omar A.

2012-01-01

459

What Is the True Color of Fresh Meat? A Biophysical Undergraduate Laboratory Experiment Investigating the Effects of Ligand Binding on Myoglobin Using Optical, EPR, and NMR Spectroscopy  

ERIC Educational Resources Information Center

|With an increased focus on integrated upper-level laboratories, we present an experiment integrating concepts from inorganic, biological, and physical chemistry content areas. Students investigate the effects of ligand strength on the spectroscopic properties of the heme center in myoglobin using UV-vis, [superscript 1]H NMR, and EPR…

Linenberger, Kimberly; Bretz, Stacey Lowery; Crowder, Michael W.; McCarrick, Robert; Lorigan, Gary A.; Tierney, David L.

2011-01-01

460

The Synthesis and Isolation of N-Tert-Butyl-2-Phenylsuccinamic Acid and N-Tert-Butyl-3-Phenylsuccinamic Acid: An Undergraduate Organic Chemistry Laboratory Experiment  

ERIC Educational Resources Information Center

The facile, high yielding synthesis of phenylsuccinamic acids is described and one of these syntheses, the reaction of phenylsuccinic anhydride with tert-butylamine, is successfully modified and adapted for use in the second-semester organic chemistry laboratory at St. John's University. Succinamic acids are compounds that contain both the amide…

Cesare, Victor; Sadarangani, Ishwar; Rollins, Janet; Costello, Dennis

2004-01-01

461

A One-Pot Self-Assembly Reaction to Prepare a Supramolecular Palladium(II) Cyclometalated Complex: An Undergraduate Organometallic Laboratory Experiment  

ERIC Educational Resources Information Center

|A laboratory experiment for students in advanced inorganic chemistry is described. Students prepare palladium(II) cyclometalated complexes. A terdentate [C,N,O] Schiff base ligand is doubly deprotonated upon reaction with palladium(II) acetate in a self-assembly process to give a palladacycle with a characteristic tetranuclear structure. This…

Fernandez, Alberto; Lopez-Torres, Margarita; Fernandez, Jesus J.; Vazquez-Garcia, Digna; Vila, Jose M.

2012-01-01

462

An Undergraduate Course in Modeling and Simulation of Multiphysics Systems  

ERIC Educational Resources Information Center

|An overview of a course on modeling and simulation offered at the Nanotechnology Engineering undergraduate program at the University of Waterloo. The motivation for having this course in the undergraduate nanotechnology curriculum, the course structure, and its learning objectives are discussed. Further, one of the computational laboratories

Ortiz-Rodriguez, Estanislao; Vazquez-Arenas, Jorge; Ricardez-Sandoval, Luis A.

2010-01-01

463

Activity Coefficients of Acetone-Chloroform Solutions: An Undergraduate Experiment. Undergraduate Experiment.  

ERIC Educational Resources Information Center

|Presents information, laboratory procedures, and results of an undergraduate experiment in which activity coefficients for a two-component liquid-vapor system are determined. Working in pairs, students can perform the experiment with 10 solutions in a given three-hour laboratory period. (Author/JN)|

Ozog, J. Z.; Morrison, J. A.

1983-01-01

464

National Undergraduate Research Observatory  

NSDL National Science Digital Library

The National Undergraduate Research Observatory (NURO) at Northern Arizona University and Lowell Observatory "is a consortium of primarily undergraduate institutions which have joined together to provide hands-on training and research experiences for undergraduate students." While the Key Projects link is under construction, users can find out about past student projects at the Undergraduate Research Experiences link. Researchers and students can request observation time and find planning and observing information. The website offers an image gallery and links to the consortium's member schools.

465

Student authoring of kinemages in biochemistry  

Microsoft Academic Search

This study compares the attitudes and achievement of students who construct annotated three-dimensional computer generated molecular images, kinemages, to students who use pre-constructed images. Achievement variables that were investigated include secondary, tertiary, and quaternary protein structure along with the nature of a protein's active site. Attitudinal topics were motivation to learn biochemistry, if the time investment for the projects was

Deborah Beech Booth

2001-01-01

466

BEDSIDE BIOCHEMISTRY: THE COST OF CONVENIENC  

Microsoft Academic Search

Summary Bedside biochemistry: the carrying out of complex analytical procedures by relatively unskilled staff close to the patient, has become fashionable since the introduction of sophisticated compact analysers over the last five years. The need for this is related more to clinical convenience than clinical necessity; the quality of analysis is likely to be poorer;

Gordon Challand MA; Principal Biochemist

467

The dark side of dioxygen biochemistry  

Microsoft Academic Search

The cellular biochemistry of dioxygen is Janus-faced. The good side includes numerous enzyme-catalyzed reactions of dioxygen that occur in respiration and normal metabolism, while the dark side encompasses deleterious reactions of species derived from dioxygen that lead to damage of cellular components. These reactive oxygen species have historically been perceived almost exclusively as agents of the dark side, but it

Joan Selverstone Valentine; Diana L Wertz; Thomas J Lyons; Lee-Loung Liou; Joy J Goto; Edith Butler Gralla

1998-01-01

468

Rhetorical structure of biochemistry research articles  

Microsoft Academic Search

This paper reports on the results of a move analysis [Swales, J. (1990). Genre analysis. Cambridge: Cambridge University Press] of 60 biochemistry research articles. First, a corpus was systematically compiled to ensure that it represents core journals in the focused discipline. Then, coding reliability analysis was conducted to demonstrate that, given a set of coding protocols and systematic training and

Budsaba Kanoksilapatham

2005-01-01

469

Biochemistry of Tungstoenzymes from Pyrococcus furiosus  

Microsoft Academic Search

The cell uses a variety of transition metals to provide greater catalytic diversity than could be achieved using only the functional groups of amino acids. The biochemistry of molybdenum and tungsten is unusual: they are the only 4d and 5d metal ions with established biological role(s). This thesis reports studies on the identification of tungstoenzymes using functional proteomics and on

E. Bol

2007-01-01

470

Jmol-Enhanced Biochemistry Research Projects  

ERIC Educational Resources Information Center

|We developed a protein research project for a one-semester biochemistry lecture class to enhance learning and more effectively train students to understand protein structure and function. During this semester-long process, students select a protein with known structure and then research its structure, sequence, and function. This project…

Saderholm, Matthew; Reynolds, Anthony

2011-01-01

471

Design for Step Motor Control System of Automatic Biochemistry Analyzer  

Microsoft Academic Search

Automatic biochemistry analyzer is a necessary instrument for clinical diagnostics. In this paper, a new scheme of concentrated control system for Automatic biochemistry analyzer based on FPGA (field programmable gate array) is put forward. As an important part of automatic biochemistry analyzer, motor control system is one of key parts. In the light of the problems of stepping motor in

Wang Xu; Huang Kaizheng; Xu Bin

2007-01-01

472

Current topics in plant biochemistry and physiology: Volume 6  

Microsoft Academic Search

These proceedings represent papers presented at the Sixth Annual Plant Biochemistry and Physiology Symposium held at the University of Missouri-Columbia, April 1-3, 1987 and hosted by the Interdisciplinary Plant Biochemistry and Physiology Group. The Interdisciplinary Plant Group was organized to facilitate research and training through interdisciplinary and cooperative approaches to problems facing Plant Biochemistry and Physiology. This annual Symposium is

D. D. Randall; R. E. Sharp; A. J. Novacky; D. G. Blevins

1987-01-01

473

Current topics in plant biochemistry and physiology: Volume 4: Proceedings  

Microsoft Academic Search

These proceedings represent papers presented at the Fourth Annual Plant Biochemistry and Physiology Symposium held at the University of Missouri-Columbia, April 10-12, 1985 and hosted by the Interdisciplinary Plant Biochemistry and Physiology Group. This Interdisciplinary Group was organized to facilitate research and training through interdisciplinary and cooperative approaches to problems facing Plant Biochemistry and Physiology. A key objective of this

D. D. Randall; D. G. Blevins; R. L. Larson

1985-01-01

474

Current topics in plant biochemistry and physiology, 1986: Volume 5  

Microsoft Academic Search

These proceedings represent papers presented at the Fifth Annual Plant Biochemistry and Physiology Symposium held at the University of Missouri-Columbia, April 2-4, 1986, and hosted by the Interdisciplinary Plant Biochemistry and Physiology Group. The Interdisciplinary Group was organized to facilitate research and training through interdisciplinary and cooperative approaches to problems facing Plant Biochemistry and Physiology. This annual symposium is a

D. D. Randall; C. D. Miles; C. J. Nelson; D. G. Blevins; J. A. Miernyk

1986-01-01

475

Teachers as Learners in a Cooperative Learning Biochemistry Class  

ERIC Educational Resources Information Center

|Upper level college students majoring in biochemistry at the University of New Mexico have the opportunity to participate in an advanced biochemistry course entitled "Biochemistry Education." This course introduces theories of teaching and learning, provides opportunities for participation in course organization, design, and assessment…

Osgood, Marcy P.; Mitchell, Steve M.; Anderson, William L.

2005-01-01

476

A Biochemistry and Molecular Biology Course for Secondary School Teachers  

ERIC Educational Resources Information Center

|This article describes a course for reinforcing the knowledge of biochemistry in secondary school science teachers. The Department of Biochemistry and Molecular Biology of the University of Barcelona designed a course to bring these teachers up to date with this discipline. In addition to updating their knowledge of biochemistry and molecular…

Fernandez-Novell, J. M.; Cid, E.; Gomis, R.; Barbera, A.; Guinovart, J. J.

2004-01-01

477

Exploring the Structure-Function Relationship of Macromolecules at the Undergraduate Level  

ERIC Educational Resources Information Center

|The biochemistry laboratory course is structured such that students work in pairs during the first half of the course. The purpose is to encourage per-peer learning processes during the students' first exposure to the software.|

Pastrana-Rios, Belinda

2004-01-01

478

Change In Undergraduate Engineering Education  

NSF Publications Database

Title : Tip 940408 CHANGE IN UNDERGRADUATE ENGINEERING EDUCATION Type : News NSF Org: OD / LPA Date ... - 1070. NEW NSF REPORT HIGHLIGHTS SIGNIFICANT CHANGE IN UNDERGRADUATE ENGINEERING EDUCATION While ...

479

Biochemistry Online: An Approach Based on Chemical Logic  

NSDL National Science Digital Library

This is an interactive web-based biochemistry text that I created for my upper division biochemistry class over the past five years. The book - Biochemistry Online: An Approach Basedon Chemical Logic - is based on a unique sequencing and approach to a one semester, or the first of a two semester, biochemistry course. I have published a manuscript describing this new approach. (Jakubowski, H. and Owen, W.G. The Teaching of Biochemistry: An Innovative Course Sequence Based on the Logic of Chemistry, Journal of Chemical Education, 75, 734-736, 1998).

Jakubowski, Henry

2012-02-20

480

Undergraduate Public Health Education  

Microsoft Academic Search

The IOM's 2003 report Who Will Keep the Public Healthy? recommended that “…all undergraduates should have access to education in public health.” They justified their recommendations stating that “public health is an essential part of the training of citizens.” The IOM recommendations have catalyzed a movement linking undergraduate public health education with arts and sciences' Liberal Education and America's Promise

Richard K. Riegelman

2008-01-01

481

Teaching the undergraduate CS Information Security Course  

Microsoft Academic Search

This paper presents a lab based approach to teaching the undergraduate CS Information Security course. Given the magnitude of real and potential loses associated with security breaches, employers increasingly expect graduates of CS programs to have an understanding of information security concepts. The infrastructure requirements for the course includes setting up a secure laboratory environment to accommodate the development of

Bradley K. Jensen; Melinda Cline; Carl Stephen Guynes

2006-01-01

482

Itineraries of Identity in Undergraduate Science  

ERIC Educational Resources Information Center

Building on recent linguistic-anthropological work that investigates the temporalities of educational processes, the article examines how a marginalized classroom identity is interactionally formed over time in an undergraduate chemistry laboratory. The analysis demonstrates how social marginalization is enacted along multiple temporal scales via…

Bucholtz, Mary; Barnwell, Brendan; Skapoulli, Elena; Lee, Jung-Eun Janie

2012-01-01

483

Centrifugal Pump Experiment for Chemical Engineering Undergraduates  

ERIC Educational Resources Information Center

The purpose of this paper is to describe a Centrifugal Pump Experiment that provided an experiential learning experience to chemical engineering undergraduates at the University of Missouri in the spring of 2010 in the Unit Operations Laboratory course. Lab equipment was used by senior students with computer-based data and control technology. In…

Vanderslice, Nicholas; Oberto, Richard; Marrero, Thomas R.

2012-01-01

484

Student Attitudes towards Laboratory Exercises in Medical Biochemistry.  

ERIC Educational Resources Information Center

|Examines student attitudes towards biochemical experiments and their effect on student learning. Finds that biochemical experiments in the medical curriculum are valuable, but efforts should be directed more towards the development of students' attitudes and approaches to the exercise. (Author/CCM)|

Cronholm, Tomas; Hoog, Jan-Olov; Martenson, Dick

2000-01-01

485

[beta]-Lactamases in the Biochemistry and Molecular Biology Laboratory  

ERIC Educational Resources Information Center

|[beta]-lactamases are hydrolytic enzymes that inactivate the [beta]-lactam ring of antibiotics such as penicillins and cephalosporins. The major diversity of studies carried out until now have mainly focused on the characterization of [beta]-lactamases recovered among clinical isolates of Gram-positive staphylococci and Gram-negative…

Amador, Paula; Prudencio, Cristina; Vieira, Monica; Ferraz, Ricardo; Fonte, Rosalia; Silva, Nuno; Coelho, Pedro; Fernandes, Ruben

2009-01-01

486

ERA-experiment ``space biochemistry''  

NASA Astrophysics Data System (ADS)

The general goal of the experiment was to study the response of anhydrobiotic (metabolically dormant) microorganisms (spores of Bacillus subtilis, cells of Deinococcus radiodurans, conidia of Aspergillus species) and cellular constituents (plasmid DNA, proteins, purple membranes, amino acids, urea) to the extremely dehydrating conditions of open space, in some cases in combination with irradiation by solar UV-light. Methods of investigation included viability tests, analysis of DNA damages (strand breaks, DNA-protein cross-links) and analysis of chemical effects by spectroscopic, electrophoretic and chromatographic methods. The decrease in viability of the microorganisms was as expected from simulation experiments in the laboratory. Accordingly, it could be correlated with the increase in DNA damages. The purple membranes, amino acids and urea were not measurably effected by the dehydrating condition of open space (in the dark). Plasmid DNA, however, suffered a significant amount of strand breaks under these conditions. The response of these biomolecules to high fluences of short wavelength solar UV-light is very complex. Only a brief survey can be given in this paper. The data on the relatively good survival of some of the microorganisms call for strict observance of COSPAR Planetary Protection Regulations during interplanetary space missions.

Dose, K.; Bieger-Dose, A.; Dillmann, R.; Gill, M.; Kerz, O.; Klein, A.; Meinert, H.; Nawroth, T.; Risi, S.; Stridde, C.

487

ERA-experiment "Space Biochemistry".  

PubMed

The general goal of the experiment was to study the response of anhydrobiotic (metabolically dormant) microorganisms (spores of Bacillus subtilis, cells of Deinococcus radiodurans, conidia of Aspergillus species) and cellular constituents (plasmid DNA, proteins, purple membranes, amino acids, urea) to the extremely dehydrating conditions of open space, in some cases in combination with irradiation by solar UV-light. Methods of investigation included viability tests, analysis of DNA damages (strand breaks, DNA-protein cross-links) and analysis of chemical effects by spectroscopic, electrophoretic and chromatographic methods. The decrease in viability of the microorganisms was as expected from simulation experiments in the laboratory. Accordingly, it could be correlated with the increase in DNA damages. The purple membranes, amino acids and urea were not measurably effected by the dehydrating condition of open space (in the dark). Plasmid DNA, however, suffered a significant amount of strand breaks under these conditions. The response of these biomolecules to high fluences of short wavelength solar UV-light is very complex. Only a brief survey can be given in this paper. The data on the relatively good survival of some of the microorganisms call for strict observance of COSPAR Planetary Protection Regulations during interplanetary space missions. PMID:11542696

Dose, K; Bieger-Dose, A; Dillmann, R; Gill, M; Kerz, O; Klein, A; Meinert, H; Nawroth, T; Risi, S; Stridde, C

1995-01-01

488

Glossary of Biochemistry and Molecular Biology  

NSDL National Science Digital Library

Provided by Portland Press, this online version of David Glick's glossary of over 3,000 Biochemistry and Molecular Biology terms offers concise (usually one sentence) definitions of both common and more obscure words. The glossary may be browsed alphabetically or searched by keyword. Definitions assume users have a basic familiarity with the biosciences, and the glossary is probably most useful to advanced students and professionals in related fields. Users are invited to submit useful terms they feel should be included.

Glick, David M.

489

Biochemistry (Moscow): Telomere, Telomerase, Cancer, and Aging  

NSDL National Science Digital Library

This Special Issue of Biochemistry (Moscow), (Volume 62 (1997), Number 11) includes several technical review articles on telomere, telomerase, cancer and aging. There has been a recent finding regarding telomerase, a gene which affects the mechanisms controlling human cell replication. The site above provides general information on telomerase, current telomere research, and the use of telemorase in medical practice. Telomerase increases the life-span of a cell, and is thus central to both aging and cancer.

1997-01-01

490

UT Austin Chemistry & Biochemistry: Resources & Movies  

NSDL National Science Digital Library

The University of Texas at Austin Department of Chemistry and Biochemistry has an excellent chemical reaction movie site. This site is broken down into three areas: Organic Chemical Reactions, Periodic Table, and Welch Scholar Program movies. In Organic Chemical Reactions various QuickTime movies can be viewed through either a standard or a UT Virtual Campus version. The MPEG Periodic Table is an interactive table; users may choose from several reaction movies for each element.

491

Comparative Biochemistry of Marsupial Erythrocytes: A Review  

Microsoft Academic Search

:   Although extensive studies have been performed on human erythrocytes, there is a shortage of information on marsupial erythrocytes.\\u000a Studies on haematology and biochemistry are useful in the ecomanagement of these animals especially those in wildlife parks\\u000a and zoos. The present review summarises our findings from ?30 species of marsupials. As marsupials show great diversity in\\u000a physical and behavioural characteristics,

N. S. Agar; N. B. Reinke; I. R. Godwin; P. W. Kuchel

2000-01-01

492

Student authoring of kinemages in biochemistry  

NASA Astrophysics Data System (ADS)

This study compares the attitudes and achievement of students who construct annotated three-dimensional computer generated molecular images, kinemages, to students who use pre-constructed images. Achievement variables that were investigated include secondary, tertiary, and quaternary protein structure along with the nature of a protein's active site. Attitudinal topics were motivation to learn biochemistry, if the time investment for the projects was worthwhile, future career benefits from participating in the project, and what was learned about protein structure. Students from five biochemistry classes at four universities were participants in this study. Two classes were placed in the experimental group and two classes were in the control group. The remaining class was randomly divided into the experimental and the control groups. The control group students visualized three-dimensional computer images during class and for homework assignments. In addition to these visualizations, the experimental group participants authored their own annotated three-dimensional computer images. A survey assessed the attitudes of all students who participated at all locations. The randomly divided class was also assessed with student interviews and with achievement on their final exam. Experimental group students responded with significantly higher differences to questionnaire items pertaining to what was learned about protein structure, future career benefits, and motivation to learn biochemistry. There were no significant differences in responses associated with the time investment, learning about the nature of a protein's active site, improvements in learning because of the images, or in achievement on the final exam.

Booth, Deborah Beech

493

A Model for an Introductory Undergraduate Research Experience  

ERIC Educational Resources Information Center

|An introductory, multidisciplinary lecture-laboratory course linked with a summer research experience has been established to provide undergraduate biology and chemistry majors with the skills needed to be successful in the research laboratory. This three-credit hour course was focused on laboratory skills and was designed to reinforce and…

Canaria, Jeffrey A.; Schoffstall, Allen M.; Weiss, David J.; Henry, Renee M.; Braun-Sand, Sonja B.

2012-01-01

494

A Model for an Introductory Undergraduate Research Experience  

ERIC Educational Resources Information Center

An introductory, multidisciplinary lecture-laboratory course linked with a summer research experience has been established to provide undergraduate biology and chemistry majors with the skills needed to be successful in the research laboratory. This three-credit hour course was focused on laboratory skills and was designed to reinforce and develop…

Canaria, Jeffrey A.; Schoffstall, Allen M.; Weiss, David J.; Henry, Renee M.; Braun-Sand, Sonja B.

2012-01-01

495

Hematology for Undergraduates  

ERIC Educational Resources Information Center

Describes a five-week introductory medical science course designed for both science and nonscience majors through integration of physiology, organic chemistry, anatomy, and biochemistry. Suggests its use as a quarter-semester, a tri-semester, or a regular semester course for students including premed and medical technicians. (CC)

Navari, Rudolph M.

1972-01-01

496

Designing Effective Undergraduate Research Experiences  

NASA Astrophysics Data System (ADS)

I present a model for designing student research internships that is informed by the best practices of the Center for Adaptive Optics (CfAO) Professional Development Program. The dual strands of the CfAO education program include: the preparation of early-career scientists and engineers in effective teaching; and changing the learning experiences of students (e.g., undergraduate interns) through inquiry-based "teaching laboratories." This paper will focus on the carry-over of these ideas into the design of laboratory research internships such as the CfAO Mainland internship program as well as NSF REU (Research Experiences for Undergraduates) and senior-thesis or "capstone" research programs. Key ideas in maximizing student learning outcomes and generating productive research during internships include: defining explicit content, scientific process, and attitudinal goals for the project; assessment of student prior knowledge and experience, then following up with formative assessment throughout the project; setting reasonable goals with timetables and addressing motivation; and giving students ownership of the research by implementing aspects of the inquiry process within the internship.

Severson, S.

2010-12-01

497

Accreditation standards for undergraduate forensic science programs  

NASA Astrophysics Data System (ADS)

Undergraduate forensic science programs are experiencing unprecedented growth in numbers of programs offered and, as a result, student enrollments are increasing. Currently, however, these programs are not subject to professional specialized accreditation. This study sought to identify desirable student outcome measures for undergraduate forensic science programs that should be incorporated into such an accreditation process. To determine desirable student outcomes, three types of data were collected and analyzed. All the existing undergraduate forensic science programs in the United States were examined with regard to the input measures of degree requirements and curriculum content, and for the output measures of mission statements and student competencies. Accreditation procedures and guidelines for three other science-based disciplines, computer science, dietetics, and nursing, were examined to provide guidance on accreditation processes for forensic science education programs. Expert opinion on outcomes for program graduates was solicited from the major stakeholders of undergraduate forensic science programs-forensic science educators, crime laboratory directors, and recent graduates. Opinions were gathered by using a structured Internet-based survey; the total response rate was 48%. Examination of the existing undergraduate forensic science programs revealed that these programs do not use outcome measures. Of the accreditation processes for other science-based programs, nursing education provided the best model for forensic science education, due primarily to the balance between the generality and the specificity of the outcome measures. From the analysis of the questionnaire data, preliminary student outcomes, both general and discipline-specific, suitable for use in the accreditation of undergraduate forensic science programs were determined. The preliminary results were reviewed by a panel of experts and, based on their recommendations, the outcomes identified were revised and refined. The results of this study were used to identify student outcomes and to suggest accreditation standards and an accreditation process for undergraduate forensic science programs based on those outcomes.

Miller, Marilyn Tebbs

498

A comprehensive Training Approach for Biomedical Engineers in Biochemistry and in vitro Diagnostics Technology  

Microsoft Academic Search

The purpose of this paper is to review 20 years (1987-2007) of experience in training young Biomedical Engineers in Biochemistry and in vitro Diagnostics (IVD) Technology. This encountering has resulted in the gradual formation of a comprehensive training package that includes lectures and laboratory practicals, supported by both, traditional and on-line digital means, such as lecture-notes, slides, videos, demos and

Basile Spyropoulos; Aris Tzavaras

2007-01-01

499

A Laboratory Program for Bioinorganic Chemistry  

ERIC Educational Resources Information Center

|Outlines a laboratory course entitled Inorganic Chemistry for Biological Sciences'' which is designed primarily for juniors in biochemistry, physiology, and soil sciences. Inclusion of relevant environmental topics is indicated. (CC)|

Ochiai, Ei-ichiro

1973-01-01

500

Bringing Nuclear Science into the Undergraduate Curriculum  

NASA Astrophysics Data System (ADS)

Think about the first time you encountered nuclear science in your formal curriculum. For most nuclear scientists this experience occurred as an undergraduate in an upper-level course in a traditional four-year institution. Because of changing student demographics, an explosion of interest in the life sciences, the end of the cold war and a variety of other factors, fewer undergraduates are encountering a traditional nuclear science course at all. For the field to remain vital, we suggest that educators in nuclear science will have to adapt to the changes in student populations and interests. To this end we now offer a variety of experiences to our undergraduate students that incorporate fundamental nuclear science. One component to our approach is to create exciting opportunities in undergraduate research, and another component involves creation of nuclear science modules that can fit within other courses. In recent years both of these components have evolved with an interdisciplinary flavor, but continue to yield students that become interested in pursuing nuclear science careers. We will discuss research opportunities offered to undergraduates at Hope College, and our success with collaborative research opportunities at a variety of extramural laboratories, as well as with our in-house research program with a low-energy accelerator. An overview of several pedagogical approaches we have adopted will also be presented, and there is clearly opportunity to pursue this approach much further. Although the examples are specific to Hope College, both components can clearly be adopted at a variety of other institutions.

Peaslee, Graham

2006-04-01