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The VASCO experiment  

NASA Astrophysics Data System (ADS)

The Intraseasonal Oscillation (ISO) of the deep convection is the most dominant and coherent component of the intraseasonal variability in the tropical atmosphere. This ISO is related to the onset and to the intensity of the Asian and the Australian monsoons and is suspected to influence also timing and strength of El Nino - Southern Oscillation (ENSO). However, the origin of ISO is still an open question, in particular for the role of air-sea interactions in the development and the characteristics of the ISO convective and dynamical perturbations. The experiment VASCO (Variability of the Atmosphere at the intra-Seasonal time scale and Coupling with the Ocean) aims to document the perturbation of the atmospheric dynamics, the surface fluxes and the ocean mixed layer due to the passage of an ISO. The experiment design is based on quasi-Lagrangian pressurised balloons and on Aeroclippers (balloons measuring both the atmospheric and oceanic surface parameters) for the atmospheric part and on moored buoys and ARGO floats to document the perturbation of the ocean mixed layer. The quasi-Lagrangian pressurised balloons and the Aeroclippers are developed in cooperation between CNES, LMD and LODYC.

Duvel, J. P.



76 FR 61688 - Vasco Winds, LLC; Supplemental Notice That Initial Market-Based Rate Filing Includes Request for...  

Federal Register 2010, 2011, 2012, 2013

...Energy Regulatory Commission [Docket No. ER11-4678-000 Vasco Winds, LLC; Supplemental Notice That Initial Market-Based...supplemental notice in the above-referenced proceeding of Vasco Winds, LLC's application for market-based rate...



Reflexiones sobre el sistema y las políticas de innovación del País Vasco  

Microsoft Academic Search

El primer capítulo de este documento compara el sistema de innovación vasco con el de otros países avanzados, basándose para ello en las estadísticas de I+D y en el indicador europeo de innovación (EIS). En segundo lugar, el sistema vasco se compara con el de otras regiones, basándose en las tipologías de regiones europeas y españolas elaboradas por el autor,

Mikel Navarro


Endurance and failure characteristics of modified Vasco X-2, CBS 600 and AISI 9310 spur gears  

NASA Technical Reports Server (NTRS)

Gear endurance tests and rolling-element fatigue tests were conducted to compare the performance of spur gears made from AISI 9310, CBS 600 and modified Vasco X-2 and to compare the pitting fatigue lives of these three materials. Gears manufactured from CBS 600 exhibited lives longer than those manufactured from AISI 9310. However, rolling-element fatigue tests resulted in statistically equivalent lives. Modified Vasco X-2 exhibited statistically equivalent lives to AISI 9310. CBS 600 and modified Vasco X-2 gears exhibited the potential of tooth fracture occurring at a tooth surface fatigue pit. Case carburization of all gear surfaces for the modified Vasco X-2 gears results in fracture at the tips of the gears.

Townsend, D. P.; Zaretsky, E. V.



Johnson-Cook Strength Model Constants for VascoMax 300 and 1080 Steels  

Microsoft Academic Search

High strength steels, VascoMax 300 and 1080 steel, are characterized under tension at strain rates of ˜1\\/s, ˜500\\/s, ˜1000\\/s, and ˜1500\\/s and at high temperatures (1000°F for Vascomax 300 and 1080 steel to 750°F) using the quai-static and split Hopkinson bar techniques. The data on 1080 steel exhibit a strain hardening response, whereas VascoMax 300 steel showed diminishing flow stress

John D. Cinnamon; N. S. Brar



Johnson-Cook Strength Model Constants for VascoMax 300 and 1080 Steels  

Microsoft Academic Search

High strength steels, VascoMax 300 and 1080, are characterized under tension at strain rates of ~1\\/s, ~500\\/s, ~1000\\/s, and ~1500\\/s and at high temperatures using the quasi-static and split Hopkinson bar techniques. The data on 1080 steel exhibited a typical strain hardening response, whereas Vasco-Max 300 steel showed diminishing flow stress beyond yielding because of localized necking in gauge section

J. D. Cinnamon; A. N. Palazotto; N. S. Brar; Z. Kennan; D. Bajaj



Johnson-Cook Strength Model Constants for VascoMax 300 and 1080 Steels  

Microsoft Academic Search

High strength steels, VascoMax 300 and 1080, are characterized under tension at strain rates of ?1\\/s, ?500\\/s, ?1000\\/s, and ?1500\\/s and at high temperatures using the quasi-static and split Hopkinson bar techniques. The data on 1080 steel exhibited a typical strain hardening response, whereas Vasco-Max 300 steel showed diminishing flow stress beyond yielding because of localized necking in gauge section

J. D. Cinnamon; A. N. Palazotto; N. S. Brar; Z. Kennan; D. Bajaj



Johnson-Cook Strength Model Constants for VascoMax 300 and 1080 Steels  

NASA Astrophysics Data System (ADS)

High strength steels, VascoMax 300 and 1080, are characterized under tension at strain rates of ~1/s, ~500/s, ~1000/s, and ~1500/s and at high temperatures using the quasi-static and split Hopkinson bar techniques. The data on 1080 steel exhibited a typical strain hardening response, whereas Vasco-Max 300 steel showed diminishing flow stress beyond yielding because of localized necking in gauge section of the tested specimens. The tension data are analyzed to determine the Johnson-Cook (J-C) strength model constants for the two steels. The flow stress values for VascoMax are adjusted to account for necking, and the corrected J-C model is developed.

Cinnamon, J. D.; Palazotto, A. N.; Brar, N. S.; Kennan, Z.; Bajaj, D.



Johnson-Cook Strength Model Constants for VascoMax 300 and 1080 Steels  

SciTech Connect

High strength steels, VascoMax 300 and 1080, are characterized under tension at strain rates of {approx}1/s, {approx}500/s, {approx}1000/s, and {approx}1500/s and at high temperatures using the quasi-static and split Hopkinson bar techniques. The data on 1080 steel exhibited a typical strain hardening response, whereas Vasco-Max 300 steel showed diminishing flow stress beyond yielding because of localized necking in gauge section of the tested specimens. The tension data are analyzed to determine the Johnson-Cook (J-C) strength model constants for the two steels. The flow stress values for VascoMax are adjusted to account for necking, and the corrected J-C model is developed.

Cinnamon, J. D.; Palazotto, A. N.; Kennan, Z. [Department of Aeronautics and Astronautics, Air Force Institute of Technology, WPAFB, OH 45433 (United States); Brar, N. S.; Bajaj, D. [University of Dayton Research Institute, Dayton, OH 45469-0182 (United States)



The Effect of Heat Treatment on the Structure and Properties of Standard and Modified Vasco X-2 Steel.  

National Technical Information Service (NTIS)

Vasco X-2 CVM is a patented high hot hardness carburizing grade gear steel. It is currently being considered for application in the next generation of Army helicopters because of its higher resistance to scuffing. Very limited metallurgical data exist for...

E. B. Kula P. J. Fopiano S. A. Oliver



The Aula Espazio and the Master of Space Science & Technology in the Universidad del País Vasco (Spain)  

NASA Astrophysics Data System (ADS)

We present the Aula Espazio, a facility dedicated to teach Space Sciences and Technology at Master and Doctorate level at the Universidad del País Vasco(Spain)and to promote development in this field in both the public and private sectors. A new University Observatory which homes a 50 cm telescope, a solar telescope and other instruments is also associated to the Aula. The most important activities of the Aula are developed within the context of the Master in Space Science and Technology offered in the Engineering School of Bilbao (Spain) which is designed to provide a link between basic research and space technology. The Master in Space Science and Technology is a one year master designed to give an overall view of the science and technology involved in space projects. It is open for graduates in Engineering and graduates in Physics. This master covers both the basic science aspects of space science and the technological development that space activities require. It is a multidisciplinary master with several University departments participating (Applied Physics, Theoretical Physics, Systems Engineering and Automatic control and Electronics and Telecommunications) and with important contributions from companies involved in space technology and Spanish public organisms in the space area. More information at: Acknowledgements: The Aula Espazio is funded by the Diputación Foral de Bizkaia/Bizkaiko Foru Aldundia, the Escuela Ténica Superior de Ingeniería de Bilbao and the Universidad del País Vasco.

Sanchez-Lavega, A.; Pérez-Hoyos, S.; Hueso, R.; Del Río-Gaztelurrutia, T.; Oleaga, A.



Teaching Space and Planetary Sciences: The Aula Espazio and the Master in Space Science, Technology and Observations at Universidad del País Vasco (Spain)  

NASA Astrophysics Data System (ADS)

We present the Aula Espazio, a facility dedicated to teach Space Sciences at Master and Doctorate level at the Universidad del País Vasco (Spain) and to promote development in this field in both the public and private sectors. A new University Observatory which homes a 50 cm telescope, a solar telescope and other instruments is associated to the Aula. We present here the teaching and outreach activities to be organized regarding Planetary Sciences. The most important ones are developed within the context of the Master in Space Science, Technology and Observations offered in the Engineering School in Bilbao (Spain) which is designed to provide a link between basic research and space technology. Detailed information about the aula can be found at: Acknowledgements: This facility is funded by the local government: Diputación Foral de Bizkaia, the Escuela Ténica Superior de Ingeniería de Bilbao and The Universidad del País Vasco.

Sanchez-Lavega, Agustin; Hueso, R.; Pérez-Hoyos, S.; del Rio, T.; Oleaga, A.



Teaching Planetary Sciences at the Universidad del País Vasco in Spain: The Aula Espazio Gela and its Master in Space Science and Technology  

NASA Astrophysics Data System (ADS)

Planetary science is a highly multidisciplinary field traditionally associated to Astronomy, Physics or Earth Sciences Departments. Spanish universities do not generally offer planetary sciences courses but some departments give courses associated to studies on Astronomy or Geology. We show a different perspective obtained at the Engeneering School at the Universidad del País Vasco in Bilbao, Spain, which offers a Master in Space Science and Technology to graduates in Engineering or Physics. Here we detail the experience acquired in two years of this master which offers several planetary science courses: Solar System Physics, Astronomy, Planetary Atmospheres & Space Weather together with more technical courses. The university also owns an urban observatory in the Engineering School which is used for practical exercises and student projects. The planetary science courses have also resulted in motivating part of the students to do their master thesis in scientific subjects in planetary sciences. Since the students have very different backgrounds their master theses have been quite different: From writing open software tools to detect bolides in video observations of Jupiter atmosphere to the photometric calibration and scientific use or their own Jupiter and Saturn images or the study of atmospheric motions of the Venus' South Polar Vortex using data from the Venus Express spacecraft. As a result of this interaction with the students some of them have been engaged to initiate Ph.D.s in planetary sciences enlarging a relative small field in Spain. Acknowledgements: The Master in Space Science and Technology is offered by the Aula Espazio Gela at the Universidad del País Vasco Engineer School in Bilbao, Spain and is funded by Diputación Foral de Bizkaia.

Hueso, R.; Sanchez-Lavega, A.; Pérez-Hoyos, S.



Teaching Planetary Sciences with the Master in Space Science and Technology at Universidad del País Vasco UPV/EHU: Theory and Practice works  

NASA Astrophysics Data System (ADS)

The Master in Space Science and Technology is a postgraduate course at the Universidad del País Vasco in Spain ( It has two elective itineraries on space studies: scientific and technological. The scientific branch is intended for students aiming to access the PhD doctorate program in different areas of space science, among them the research of the solar system bodies. The theoretical foundations for the solar system studies are basically treated in four related matters: Astronomy and Astrophysics, Physics of the Solar System, Planetary Atmospheres, and Image Processing and Data Analysis. The practical part is developed on the one hand by analyzing planetary images obtained by different spacecrafts from public archives (e. g. PDS), and on the other hand from observations obtained by the students employing the 50 cm aperture telescope and other smaller telescopes from the Aula EspaZio Gela Observatory at the Engineering Faculty. We present the scheme of the practice works realized at the telescope to get images of the planets in different wavelengths pursuing to study the following aspects of Planetary Atmospheres: (1) Data acquisition; (2) Measurements of cloud motions to derive winds; (3) Measurement of the upper cloud reflectivity at the different wavelengths and position in the disk to retrieve the upper cloud properties and vertical structure. The theoretical foundations accompanying these practices are then introduced: atmospheric dynamics and thermodynamics, and the radiative transfer problem. Acknowledgments: This work was supported by Departamento de Promoción Económica of Diputación Foral Bizkaia through a grant to Aula EspaZio Gela at E.T.S. Ingeniería (Bilbao, Spain).

Sanchez-Lavega, Agustin; Hueso, R.; Perez-Hoyos, S.




Microsoft Academic Search

A tecnologia está mais acessível ao produtor. O valor de um computador hoje equivale a 1 tonelada de adubo (uréia). A medida que o custo da tecnologia caiu, os valores dos fatores de produção – fertilizantes e salários – subiram significamente. Assim, é vital o produtor encontrar formas de otimizar o uso de insumos e melhorar a produtividade da mão-de-obra

Margarete Boteon; Rafaela Cristina Da Silva; Joao Paulo Bernardes Deleo




Microsoft Academic Search

Foi o trabalho sistemático com a técnica da análise do caráter que levou Reich a descobrir que os distúrbios psico-emocionais apresentam como equivalente somático, a couraça muscular. Couraças são tensões que se formam ao longo da vida, cuja função é proteger o indivíduo (ego) de experiências dolorosas e ameaçadoras. Inclui tensão ou flacidez crônicas da musculatura esquelética que são resultados

José Henrique Volpi; Maria Beatriz de Paula



Microsoft Academic Search

Na generalidade dos países desenvolvidos, os sistemas públicos de pensionamento têm-se confron- tado com o aumento da longevidade e com o decréscimo da natalidade das suas populações. Quase invariavelmente, a reacção dos decisores políticos traduziu-se no aumento da idade legal da reforma, isto é, da idade em que os trabalhadores são investidos no direito a receber uma pensão completa; na

Pedro Martins; Álvaro Novo; Pedro Portugal



Berengario da Carpi.  


Berengario da Carpi was magister of anatomy and surgery at the University of Bologna from 1502 to 1527. Eustachio and Falloppia defined him as 'the restaurator of anatomy'. He was a great surgeon, anatomist and physician of illustrious patients including Lorenzo II dei Medici, Giovanni dalle Bande Nere, Galeazzo Pallavicini, Cardinal Colonna, and Alessandro Soderini. He had strong links to the intellectuals of his time (Forni, Bonamici, Manuzio, Pomponazzi) as well as with the Medici family. He was respected by the Popes Julius II, Leo X and Clement VII. His main contributions are the Isogogae Breves, De Fractura calvae sive cranei, and the illustrated Commentaria on the Anatomy of Mondino de Liucci, a textbook utilized for more than 200 years, which Berengario aimed to restore to its initial text. The Commentaria constitutes the material for the last part of this paper which concludes with a personal translation of some passages on 'The kidney', where the author gives poignant examples of experimental ingenuity. PMID:10213820

De Santo, N G; Bisaccia, C; De Santo, L S; De Santo, R M; Di Leo, V A; Papalia, T; Cirillo, M; Touwaide, A



El metabolismo social del País Vasco desde el análisis de flujos materiales  

Microsoft Academic Search

La desvinculación de la mejora del bienestar respecto del uso de los recursos y de la contaminación es esencial para lograr un desarrollo sostenible. Para avanzar en esta dirección es preciso partir del conocimiento del metabolismo de la sociedad. Es decir, debemos analizar en qué medida nuestras sociedades “ingieren” materias primas, que son “metabolizadas” para producir bienes y servicios, y

Iñaki Arto



Diseño del saneamiento de Urdaibai (País Vasco) bajo una perspectiva de preservación ambiental del litoral  

Microsoft Academic Search

Los sistemas de saneamiento unitarios, del mismo modo que cualquier obra de infraestructura dispuesta a soportar fenómenos naturales, pre- sentan, inevitablemente, una determinada probabilidad de fallo, igual, a la de que los efectos producidos por estos superen a los considerados en su diseño. Por tanto, el comportamiento general de los mismos sólo puede evaluarse en términos probabilísticos. Siendo la lluvia



Fluorine substitution enhanced photovoltaic performance of a D-A(1)-D-A(2) copolymer.  


A new alternating donor-acceptor (D-A1-D-A2) copolymer containing two electron-deficient moieties, isoindigo and quinoxaline, was synthesized. The photovoltaic performance of this polymer could be improved by incorporating fluorine atoms into the quinoxaline units, resulting in an efficiency of 6.32%. This result highlights the attractive promise of D-A1-D-A2 copolymers for high-performance bulk heterojunction solar cells. PMID:24000353

Dang, Dongfeng; Chen, Weichao; Yang, Renqiang; Zhu, Weiguo; Mammo, Wendimagegn; Wang, Ergang




Microsoft Academic Search

RESUMO - A região cacaueira da Bahia vem enfrentando uma crise sem precedente em sua história com a redução continuada da renda e emprego em virtude de problemas enfrentados pela cultura do cacau. O crescimento do parque agroindustrial de polpa de frutas na região poderá contribuir na diversificação da economia regional ao criar uma opção de mercado para a produção

Antônio Carlos de Araújo; Lúcia Maria Ramos Silva; Ahmad Saeed Khan; José B. V. Leite; Aurélio F. Macedo


Obstáculos e potencialidades à competitividade das MPEs de calçados da Paraíba- um estudo da cooperação do aprendizado e da inovação  

Microsoft Academic Search

O objetivo deste trabalho é avaliar os obstáculos e potencialidades das micro e pequenas empresas da Paraíba, através do estudo da cooperação, aprendizado e inovação. Essas empresas estão concentradas espacialmente nos Arranjos Produtivos Locais (APLs) de Campina Grande e de Patos, onde há uma diversidade de atores públicos e privados, além de uma base institucional relevante. Para tanto, utilizou-se um

Janaina da Silva Alves



Microsoft Academic Search

O objetivo principal deste trabalho foi analisar o termo de consentimento para laqueadura de trompas perante a autonomia do corpo da mulher analisando-se os princípios e garantias constitucionais conseguidos a duras penas ao longo de muitos anos. Teve como método uma pesquisa bibliográfica envolvendo legislação e Doutrina, bem como contato direto com instituições e profissionais habilitados na área da saúde

Marcelo Nunes Apolinário; Carmen Liliam Rodrigues Arnoni




Microsoft Academic Search

RESUMO Neste trabalho, avaliou-se o efeito da posição da linha lateral de um equipamento de irrigação do tipo pivô central na uniformidade de distribuição de água, lâmina de irrigação aplicada, eficiência de aplicação, eficiência de armazenamento, adequabilidade da irrigação e velocidade de deslocamento da torre final. Foram avaliadas três posições: aclive d e 3 ,87%, nível e d eclive de

João Luis Zocoler; Luis Eduardo; Vilar Cesar; Luiz Sérgio Vanzela


Lyman Spectroscopy of Hot DA White Dwarfs  

NASA Astrophysics Data System (ADS)

Several hot (\\teff\\ > 20,000 K) DA white dwarfs were observed with the Hopkins Ultraviolet Telescope (HUT) during the recent ASTRO-2 mission. This is the first time that the full hydrogen Lyman series has been observed for a significant number of white dwarfs. The observations were undertaken as a critical test of Stark broadening theory and the Hummer-Mihalas occupation probability formalism (Hummer, D.G. and Mihalas, D. 1988, ApJ 331, 794); any departures were expected to be of much greater magnitude in the Lyman lines, given that they are much stronger than the Balmer lines. To span the widest possible range in \\teff\\ and \\logg the targets included Wolf 1346, GD 50, GD 71, GD 153, GD 394, RE0512-004, RE1738+669, HZ 43, and G191-B2B. These include one of the coolest DA without Lyman satellite features, Wolf 1346 (20,000 K), and the hottest known DA, RE1738+669 (95,000 K); one of the least massive DA, RE0512-004 (\\logg\\ = 7.3), and the most massive known DA, GD 50 (\\logg\\ = 9.2). (The temperatures and gravities listed are from pure H model fits to our optical spectra). The flight data are now being reduced, after which we will perform detailed comparisons of the Lyman and Balmer line profiles with models. We will be reporting on the initial results of that comparison.

Finley, David S.



Mulheres atletas: re-significações da corporalidade feminina  

Microsoft Academic Search

R R R R Resumo esumo esumo esumo esumo: A participação esportiva das mulheres contribui para uma re-significação da corporalidade feminina? Lembrando da idéia de Susan Brownmiller, para quem a feminilidade representa, na sociedade moderna, uma \\

Miriam Adelman



Variable Importance in MANOVA and DA.  

ERIC Educational Resources Information Center

Some interpretations of relative variable importance in the contexts of multivariate analysis of variance (MANOVA) and discriminant analysis (DA) are presented. Some indices potentially useful for the interpretations are presented, and the assessment of variable importance is illustrated using real data sets. Both descriptive discriminant analysis…

Huberty, Carl J.; Wisenbaker, Joseph M.


IrDA: past, present and future  

Microsoft Academic Search

The core Internet technologies were in the hands of the research community 10 or more years before the World Wide Web happened and popularized the Internet as a place to find information, access service, and trade. The Infrared Data Association has been in existence for over six years. Products embedding the communication technology the IrDA defines have been around for

Stuart Williams



Excitation by gravity of DA and EDC waves  

NASA Astrophysics Data System (ADS)

Excitation of dust-acoustic (DA) and electrostatic dust-cyclotron (EDC) waves by gravity in a collisional plasma is considered. The fall of charged dust grains through the plasma may excite both DA and EDC waves. The case of an ionospheric plasma is considered, for which it is found that the EDC mode has a substantially larger growth than the DA mode.

D'Angelo, N.




Microsoft Academic Search

Ciro dos Anjos Abdias and A menina do sobrado O presente trabalho apresenta um breve estudo sobre os direitos fundamentais da mulher, fazendo um retrocesso de sua posição na sociedade, desde os tempos bíblicos, quando surgiu o mito de que fora responsável pela queda do homem, iniciando-se sua discriminação. Foi feito um levantamento da situação da mulher em diversos países,




Microsoft Academic Search

Resumo: Procura o presente estudo traçar um breve panorama da situação da mulher no que concerne à proteção da sua dignidade. Em primeiro lugar, faz-se um breve histórico sobre o estabelecimento de convenções e outros instrumentos internacionais de proteção à dignidade da mulher. Em segundo lugar, apontam-se os grandes desafios enfrentados na atualidade para a efetiva proteção dos direitos da

Juliana Oliveira Domingues; Santa Marcelina


Immunochemical and immunohistochemical studies, using antisera against porcine 25 kDa amelogenin, 89 kDa enamelin and the 13–17 kDa nonamelogenins, on immature enamel of the pig and rat  

Microsoft Academic Search

Enamel proteins were extracted from the newly formed layer of immature porcine enamel, and the 25 kDa amelogenin, 89 kDa enamelin and 13–17 kDa nonamelogenins were purified. Specific antisera were raised against these proteins. Antibodies specific to the C-terminal region (residues 149–173) of the 25 kDa amelogenin were generated by absorption of the anti-25 kDa amelogenin serum with 20 kDa

T. Uchida; T. Tanabe; M. Fukae; M. Shimizu; Mo Yamada; K. Miake; S. Kobayashi



The Finuda Experiment at DA?NE  

NASA Astrophysics Data System (ADS)

The FINUDA (FIsica NUcleare a DA?NE) experiment was built in order to study the interactions of stopped K- on nuclei, with emphasis on Hypernuclear spectroscopy and Hypernuclei decays. Due to the nice performances of the spectrometer, it proved also suited to study, more generally, final states with several particles and able to recostruct secondary vertices, hence greatly enlarging the physical topics that could be addressed.

Lucherini, V.



The Finuda Experiment at DA?NE  

NASA Astrophysics Data System (ADS)

The FINUDA (FIsica NUcleare a DA?NE) experiment was built in order to study the interactions of stopped K- on nuclei, with emphasis on Hypernuclear spectroscopy and Hypernuclei decays. Due to the nice performances of the spectrometer, it proved also suited to study, more generally, final states with several particles and able to recostruct secondary vertices, hence greatly enlarging the physical topics that could be addressed.

Lucherini, V.


The Finuda Experiment on DA?NE  

NASA Astrophysics Data System (ADS)

FINUDA (FIsica NUcleare a DA?ne) is a spectrometer built to study the interactions of stopped K- on nuclei, with emphasis on spectroscopy and decay of ?-hypernuclei. Due to the nice performances of the apparatus and its ability to reconstruct secondary vertices, it proved suited to study also final states with several particles (including neutron), hence greatly enlarging the physical topics that could be addressed.

Lucherini, Vincenzo


Raízes pós-mitológicas da Astronomia Clássica  

NASA Astrophysics Data System (ADS)

Apresentamos como a invenção de um modelo astronômico e cosmológico fundado em causas naturais foi um processo gestado num ambiente cultural específico, o do advento do pensamento pós-mitológico, que tem como sua raiz mais funda a trajetória particular da Grécia Antiga, com ênfase na resolução de uma grave crise social e a evolução do mundo clássico para o contexto espiritual de um império universal.

di Bartolo, L.; Villas da Rocha, J. F.




Microsoft Academic Search

ABSTRACT:This paper describes the dynamic tests performed on a large cable-stayed bridge, Vasco da Gama Bridge, on the basis of a non-conventional testing system, comprehending several independent accelerographs conveniently synchronised by a laptop, as well as a laser interferometry system for non- contact dynamic measurements in stay cables. This system showed to be rather portable, efficient and accurate, leading to

A. Cunha; E. Caetano; R. Delgado


Leonardo da Vinci's studies of the heart.  


Leonardo da Vinci's detailed drawings are justly celebrated; however, less well known are his accounts of the structures and functions of the organs. In this paper, we focus on his illustrations of the heart, his conjectures about heart and blood vessel function, his experiments on model systems to test those conjectures, and his unprecedented conclusions about the way in which the cardiovascular system operates. In particular, da Vinci seems to have been the first to recognize that the heart is a muscle and that systole is the active phase of the pump. He also seems to have understood the functions of the auricles and pulmonary veins, identified the relationship between the cardiac cycle and the pulse, and explained the hemodynamic mechanism of valve opening and closure. He also described anatomical variations and changes in structure and function that occurred with age. We outline da Vinci's varied career and suggest ways in which his personality, experience, skills and intellectual heritage contributed to these advances in understanding. We also consider his influence on later studies in anatomy and physiology. PMID:23044431

Shoja, Mohammadali M; Agutter, Paul S; Loukas, Marios; Benninger, Brion; Shokouhi, Ghaffar; Namdar, Husain; Ghabili, Kamyar; Khalili, Majid; Tubbs, R Shane



A computational theory of da Vinci stereopsis.  


In binocular vision, occlusion of one object by another gives rise to monocular occlusions—regions visible only in one eye. Although binocular disparities cannot be computed for these regions, monocular occlusions can be precisely localized in depth and can induce the perception of illusory occluding surfaces. The phenomenon of depth perception from monocular occlusions, known as da Vinci stereopsis, is intriguing, but its mechanisms are not well understood. We first propose a theory of the mechanisms underlying da Vinci stereopsis that is based on the psychophysical and computational literature on monocular occlusions. It postulates, among other principles, that monocular areas are detected explicitly, and depth from occlusions is calculated based on constraints imposed by occlusion geometry. Next, we describe a biologically inspired computational model based on this theory that successfully reconstructs depth in a large range of stimuli and produces results similar to those described in the psychophysical literature. These results demonstrate that the proposed neural architecture could underpin da Vinci stereopsis and other stereoscopic percepts. PMID:24914063

Tsirlin, Inna; Wilcox, Laurie M; Allison, Robert S



A Influência da Escola e da Mídia na Obtenção dos Conhecimentos Astronômicos  

NASA Astrophysics Data System (ADS)

O universo em que vivemos possui segredos jamais imaginados pelo homem, fruto da pequena proporção que ocupamos diante de um cosmos infinito. A astronomia funciona como um instrumento que sacia a sede humana em desvendar esses segredos, estudando as galáxias, quasares, pulsares além da existência de todos os elementos que preenchem o espaço cósmico, o vácuo, não mais vazio como se pensou em outros tempos. A mídia explora esse campo de forma mística e também científica. A difusão adequada e dentro dos padrões educacionais vigentes, de conceitos astronômicos, se faz necessária, devido sua presença nos conteúdos escolares, centros de ciência e na mídia, de modo geral. A reforma do Currículo Básico da Escola Pública da maioria dos Estados brasileiros introduziu conceitos astronômicos desde a pré-escola até o Ensino Médio. Num estudo realizado com 1.032 alunos do Ensino Médio de seis escolas estaduais, revelou-se através da aplicação de um questionário que abordava conheciment os básicos em astronomia, como estes foram adquiridos e qual era a infra-estrutura oferecida pela escola: para 66,2% dos entrevistados o professor já fez alguma apresentação a respeito de astronomia. O presente estudo revelou também que 44,5% dos alunos entrevistados adquiriram seus conhecimentos astronômicos na escola, 28,1% através da televisão e 27,4% afirmaram que seus conhecimentos foram obtidos através de outros tipos de mídia (internet, filmes e revistas). Embora o estudo revele claramente que há certo equilíbrio na forma em que os alunos obtiveram seus conhecimentos astronômicos, a mídia ainda explora e muitas vezes desperta o interesse pela astronomia com assuntos que não fazem parte de nosso cotidiano como, por exemplo, o homem na Lua, os projetos espaciais e a primeira viagem de um astronauta brasileiro no espaço. Porém, em certos casos, a mídia pode promover conhecimentos incompletos ou mesmo equivocados sobre os conceitos que envolvam a astronomia, como por exemplo a propagação do som no vácuo observado na maioria dos filmes de f icção científica ou da existência das estações do ano em função da distância da Terra ao Sol, em conformidade com o questionário aplicado.

da Cunha, W. S.; Voelzke, M. R.; Amaral, L. H.



Análise da Evolução e da Estrutura da Siderurgia Brasileira e do Uso de Carvão Vegetal no período de 1980 a 2006  

Microsoft Academic Search

O presente trabalho analisa a evolução e estrutura da siderurgia brasileira e do uso do carvão vegetal no período de 1980 a 2006. Os objetivos específicos são: 1) analisar a evolução da produção, consumo, exportação e importação dos principais produtos elaborados por esta indústria, separando os que usam carvão vegetal e os que usam carvão mineral; e 2) caracterizar a

Thais Hortense De Carvalho; Carlos Jose Caetano Bacha



The ATLAS PanDA Monitoring System and its Evolution  

Microsoft Academic Search

The PanDA (Production and Distributed Analysis) Workload Management System is used for ATLAS distributed production and analysis worldwide. The needs of ATLAS global computing imposed challenging requirements on the design of PanDA in areas such as scalability, robustness, automation, diagnostics, and usability for both production shifters and analysis users. Through a system-wide job database, the PanDA monitor provides a comprehensive

A Klimentov; P Nevski; M Potekhin; T Wenaus



Photospheric metals in hot DA white dwarfs  

NASA Astrophysics Data System (ADS)

Previous observations of the soft X-ray and EUV region with the Einstein, EXOSAT, and ROSAT satellites have indicated the presence of photospheric absorbers in most DA white dwarfs with {T_eff} ga 40000K. Several of these objects have now been observed with the Extreme Ultraviolet Explorer (EUVE). Since the detection of individual metal lines is rather difficult with EUVE spectra we chose the well studied DA G191-B2B as reference object for the analysis of other white dwarfs. In spectra obtained with the GHRS of the Hubble Space Telescope photospheric lines of nitrogen, silicon, iron, and nickel could be detected. With the abundances determined from these observations we were able to reproduce the EUVE spectrum of G191-B2B. The main source of EUV opacity turned out to be iron, followed by nickel. The analysis of both the EUV continuum and the hydrogen Balmer lines led to {T_eff} = 56000 +/- 2000K. For the analysis of other DA white dwarfs we used the same relative abundances as for G191-B2B but introduced a free scaling factor (``metallicity'') for the total amount of metals. From EUVE spectra we determined relative metallicities for 20 objects with {T_eff} ga 40000K. The main result is that all DAs with {T_eff} ga 50000K contain additional photospheric absorbers, whereas at lower temperatures all observations, with the exception of GD394, are compatible with pure hydrogen atmospheres. As in the case of G191-B2B, iron determines also the EUV opacity in the objects at {T_eff} ga 50000K. This is in agreement with radiative levitation theory which predicts the support of iron above this temperature. Based on observations with the HST and EUVE satellites, and on optical spectra obtained at the DSAZ Calar Alto

Wolff, B.; Koester, D.; Dreizler, S.; Haas, S.




Microsoft Academic Search

1. Introdução Este estudo tem por objetivo fazer uma reflexão, sob a luz da análise do discurso da linha francesa, acerca do humor na literatura, mais precisamente, da linguagem transgressiva que se instaura no discurso literário-teatral em decorrência de fenômenos como ironia e paródia. Esse enfoque será norteado a partir da peça Calabar - o elogio da traição (1973), de

Manuella Felicíssimo


Levantamento dos Fatores Limitantes da Produtividade Leiteira na Regi- ão de Anápolis-Go  

Microsoft Academic Search

O presente trabalho foi realizado no município de Anápolis-GO no mês de julho de 1995. Consistiu de uma avaliação da atividade pecuária em fazendas leiteiras com o objetivo de, através da análise dos principais índices zootécnicos, determinar quais os fatores limitantes para o aumento da produtividade. Constou da aplicação de um questionário de levantamento sócio-econômico da produção em uma amostra



40 CFR 60.49Da - Emission monitoring.  

Code of Federal Regulations, 2011 CFR

...60.44Da(d)(1), and 60.44Da(e). (1) The owner or operator of an affected facility with electricity generation shall install, calibrate, maintain, and operate a wattmeter; measure gross electrical output in MWh on...



Research on Wireless OPC DA Server for Modbus  

Microsoft Academic Search

Based on the study of the drawback of traditional wired automatic control system and current wireless remote monitoring system for Modbus, and the utilization of a Uniform Wireless OPC DA Server, a new solution to construct a wireless OPC DA server for Modbus was given out. It used mobile communication networks and OPC technology. Also, Modbus request command strategy was

Xiaoping Zhou; Xiaoxuan Ma; Hantao Zhang



The Case: Bunche-Da Vinci Learning Partnership Academy  

ERIC Educational Resources Information Center

The Bunche-Da Vinci case described in this article presents a situation at Bunche Elementary School that four theorists were asked to address in their evaluation designs (see EJ791771, EJ719772, EJ791773, and EJ792694). The Bunche-Da Vinci Learning Partnership Academy, an elementary school located between an urban port city and a historically…

Eisenberg, Nicole; Winters, Lynn; Alkin, Marvin C.



Asteroid 2012 DA14 To Whiz Past Earth Safely  

NASA Video Gallery

The small near-Earth asteroid 2012 DA14 will pass very close to Earth on Feb. 15, 2013. Asteroid 2012 DA14 will be closest to Earth at about 11:24 a.m. PST (2:24 p.m. EST and 1924 UTC), on Feb. 15,...


DA-6034 Induces [Ca2+]i Increase in Epithelial Cells  

PubMed Central

DA-6034, a eupatilin derivative of flavonoid, has shown potent effects on the protection of gastric mucosa and induced the increases in fluid and glycoprotein secretion in human and rat corneal and conjunctival cells, suggesting that it might be considered as a drug for the treatment of dry eye. However, whether DA-6034 induces Ca2+ signaling and its underlying mechanism in epithelial cells are not known. In the present study, we investigated the mechanism for actions of DA-6034 in Ca2+ signaling pathways of the epithelial cells (conjunctival and corneal cells) from human donor eyes and mouse salivary gland epithelial cells. DA-6034 activated Ca2+-activated Cl- channels (CaCCs) and increased intracellular calcium concentrations ([Ca2+]i) in primary cultured human conjunctival cells. DA-6034 also increased [Ca2+]i in mouse salivary gland cells and human corneal epithelial cells. [Ca2+]i increase of DA-6034 was dependent on the Ca2+ entry from extracellular and Ca2+ release from internal Ca2+ stores. Interestingly, these effects of DA-6034 were related to ryanodine receptors (RyRs) but not phospholipase C/inositol 1,4,5-triphosphate (IP3) pathway and lysosomal Ca2+ stores. These results suggest that DA-6034 induces Ca2+ signaling via extracellular Ca2+ entry and RyRs-sensitive Ca2+ release from internal Ca2+ stores in epithelial cells.

Yang, Yu-Mi; Park, Soonhong; Ji, HyeWon; Kim, Tae-im; Kim, Eung Kweon; Kang, Kyung Koo



Berengario da Carpi: a pioneer in neurotraumatology.  


Berengario da Carpi was one of the most famous physicians of the 16th century, a recognized master of anatomy and surgery, an emblematic "Renaissance man" who combined his medical experience and engineering knowledge to design new surgical instruments, and effectively used the arts of writing and drawing to describe state-of-the-art medicine and provide illustrations of anatomical structures. His greatest contribution to medicine was to write the most important work on craniocerebral surgery of the 16th century, the Tractatus de Fractura Calvae sive Cranei (Treatise on Fractures of the Calvaria or Cranium), in which he described an entire set of surgical instruments to be used for cranial operations to treat head traumas that became a reference for later generations of physicians. This was a systematic treatise covering the mechanisms, classification, and medical and surgical treatment of head traumas, and can be considered a milestone in the history of neurotraumatology. PMID:21128734

Di Ieva, Antonio; Gaetani, Paolo; Matula, Christian; Sherif, Camillo; Skopec, Manfred; Tschabitscher, Manfred



Sobre a largura da última superfície de espalhamento  

NASA Astrophysics Data System (ADS)

De acordo com o modelo do "Big-Bang", no universo primordial a matéria estava em equilíbrio térmico com a radiação. Com a expansão a temperatura da radiação cai. Quando a temperatura chega em torno dos 4.000K, os espalhamentos diminuem, começando a recombinação dos prótons e elétrons em Hidrogênio neutro (era conhecida como da recombinação). Ao final da recombinação, os fótons se propagam livremente sofrendo, em princípio, somente os efeitos do "redshift" cosmológico. Esses fótons nos alcançam hoje como a radiação cósmica de fundo (RCF), e parecem vir de uma superfície esférica ao nosso redor, tal que o raio dela é a distância que ele viajou desde seu último espalhamento na época da recombinação. Naturalmente, esse processo não ocorreu abruptamente, implicando na existência de uma largura no espaço dos "redshifts" que deve depender do modelo cosmológico específico e dos processos físicos considerados. Neste trabalho analisamos os efeitos de diferentes modelos - a saber, aqueles com decaimento do vácuo L(t), criação de matéria, quintessência e gás de Chaplygin - sobre a última superfície de espalhamento da RCF, em particular sua largura e a função visibilidade, que determina a probabilidade de um fóton ter tido seu último espalhamento num "redshift" z. No caso particular dos modelos com decaimento do vácuo, existe uma forte dependência da função visibilidade com L(t). Tais efeitos poderão ser testados através da análise dos resultados de experimentos mais precisos que estão atualmente em andamento, como por exemplo, o WMAP.

Nobre, M. A. S.; Pires, N.; Lima, J. A. S.



HeDaM: The Herschel Database in Marseille  

NASA Astrophysics Data System (ADS)

The Herschel Database in Marseille (HeDaM) is an information system operated by the Laboratoire d'Astrophysique de Marseille (LAM) datacenter — CeSAM. Gathering data from various Herschel Space Observatory surveys, HeDaM serves both as a collaboration tool between scientists and as a way to distribute public data to a wider community. With HeDaM, users have access to the direct download of catalogs and maps, but also to value-adding services such as: multi-? FITS cut-outs, integration with Aladin applet and Topcat, and list-based cone searches.

Roehlly, Y.; Buat, V.; Heinis, S.; Moreau, C.; Gimenez, S.



Metal Lines in DA White Dwarfs  

NASA Astrophysics Data System (ADS)

We report Keck telescope HIRES echelle observations of DA white dwarfs in a continuation of an extensive search for metals. These spectra are supplemented with new JHK magnitudes that are used to determine improved atmospheric parameters. Of the DA white dwarfs not in binary or common proper motion systems, about 25% show Ca II lines. For these, Ca abundances are determined from comparison with theoretical equivalent widths from model atmosphere calculations; in a few cases we also obtain Mg, Fe, Si, and Al abundances. If Ca is not observed, we generally determine very stringent upper limits. We compare the data to predictions of previously published models involving the accretion/diffusion of interstellar matter and of comets. The derived abundances are not obviously compatible with the predictions of either model, which up to now could only be tested with traces of metals in helium-rich white dwarfs. By modifying certain assumptions in the published interstellar accretion model we are able to match the distribution of the elements in the white dwarf atmospheres, but, even so, tests of other expectations from this scenario are less successful. Because comet accretion appears unlikely to be the primary cause of the DAZ phenomenon, the data suggest that no more than about 20% of F-type main-sequence stars are accompanied by Oort-like comet clouds. This represents the first observational estimate of this fraction. A plausible alternative to the accretion of cometary or interstellar matter is disruption and accretion of asteroidal material, a model first suggested in 1990 to explain excess near-infrared emission from the DAZ G29-38. An asteroidal debris model to account for the general DAZ phenomenon does not presently disagree with the HIRES data, but neither is there any compelling evidence in support of such a model. The HIRES data indicate that in close red dwarf/white dwarf binaries not known to be cataclysmic variables there is, nonetheless, significant mass transfer, perhaps in the form of a wind flowing off the red dwarf. As a by-product we find from the kinematics of GD 165 a likely age of more than 2 Gyr for its probable brown dwarf companion GD 165B. This paper is based in part on observations obtained at the Calar Alto Observatory of the Deutsch-Spanisches Astronomisches Zentrum and at the W. M. Keck Observatory, which is operated as a scientific partnership among the California Institute of Technology, the University of California, and NASA. The Observatory was made possible by the generous financial suppport of the W. M. Keck Foundation. We have made use of the SIMBAD database at CDS.

Zuckerman, B.; Koester, D.; Reid, I. N.; Hünsch, M.



Circumstellar features in hot DA white dwarfs  

NASA Astrophysics Data System (ADS)

We present a phenomenological study of highly ionized, non-photospheric absorption features in high spectral resolution vacuum ultraviolet spectra of 23 hot DA white dwarfs. Prior to this study, four of the survey objects (Feige 24, REJ 0457-281, G191-B2B and REJ 1614-085) were known to possess these features. We find four new objects with multiple components in one or more of the principal resonance lines: REJ 1738+665, Ton 021, REJ 0558-373 and WD 2218+706. A fifth object, REJ 2156-546, also shows some evidence of multiple components, though further observations are required to confirm the detection. We discuss possible origins for these features including ionization of the local interstellar environment, the presence of material inside the gravitational well of the white dwarf, mass loss in a stellar wind and the existence of material in an ancient planetary nebula around the star. We propose ionization of the local interstellar medium as the origin of these features in G191-B2B and REJ 1738+665, and demonstrate the need for higher-resolution spectroscopy of the sample, to detect multiple interstellar medium velocity components and to identify circumstellar features that may lie close to the photospheric velocity.

Bannister, N. P.; Barstow, M. A.; Holberg, J. B.; Bruhweiler, F. C.




Microsoft Academic Search

RESUMO A presença cada vez mais marcante das tecnologias de comunicação e informação no cotidiano dos sujeitos tem alertado aos profissionais da educação para a necessidade de construir e utilizar a potencialidade dessas ferramentas. O objetivo deste artigo é mostrar que os pressupostos da Teoria da Complexidade, num ambiente virtual, contribuem de forma decisiva para potencializar a aprendizagem da língua

Dulci Marlise BOETTCHER


Tree Branching: Leonardo da Vinci's Rule versus Biomechanical Models  

PubMed Central

This study examined Leonardo da Vinci's rule (i.e., the sum of the cross-sectional area of all tree branches above a branching point at any height is equal to the cross-sectional area of the trunk or the branch immediately below the branching point) using simulations based on two biomechanical models: the uniform stress and elastic similarity models. Model calculations of the daughter/mother ratio (i.e., the ratio of the total cross-sectional area of the daughter branches to the cross-sectional area of the mother branch at the branching point) showed that both biomechanical models agreed with da Vinci's rule when the branching angles of daughter branches and the weights of lateral daughter branches were small; however, the models deviated from da Vinci's rule as the weights and/or the branching angles of lateral daughter branches increased. The calculated values of the two models were largely similar but differed in some ways. Field measurements of Fagus crenata and Abies homolepis also fit this trend, wherein models deviated from da Vinci's rule with increasing relative weights of lateral daughter branches. However, this deviation was small for a branching pattern in nature, where empirical measurements were taken under realistic measurement conditions; thus, da Vinci's rule did not critically contradict the biomechanical models in the case of real branching patterns, though the model calculations described the contradiction between da Vinci's rule and the biomechanical models. The field data for Fagus crenata fit the uniform stress model best, indicating that stress uniformity is the key constraint of branch morphology in Fagus crenata rather than elastic similarity or da Vinci's rule. On the other hand, mechanical constraints are not necessarily significant in the morphology of Abies homolepis branches, depending on the number of daughter branches. Rather, these branches were often in agreement with da Vinci's rule.

Minamino, Ryoko; Tateno, Masaki




Microsoft Academic Search

In the present article we review engineering and research efforts conducted by a group of volunteers with the help of advanced engineering commercial software (CFD-ACE+, ANSYS, CFD-FASTRAN, Matlab\\/Simulink, Autodesk Inventor, Maple) in support of the da Vinci Project, the first Canadian competitor in the International X Prize Competition. The da Vinci Project has an objective of launching the first commercial

Vladimir Kudriavtsev; Brian Feeney; Max Buneta; James Porcher; Asier Ania; Michael Trauttmansdorff; Ta-Liang Hsu; Marek Krzeminski; Kalman Rooz


Variação da qualidade da madeira entre grupos fenotípicos de clones de Eucalyptus urophylla S.T. Blake da Ilha de Flores, Indonesia Wood quality variation among phenotypic groups of clones Eucalyptus urophylla S.T. Blake from Flores Island, Indonesia  

Microsoft Academic Search

RESUMO: O objetivo do presente trabalho foi estudar parâmetros de qualidade da madeira (densidade básica, comprimento, largura, diâmetro e espessura da parede das fibras) de clones de Eucalyptus urophylla, da Ilha de Flores, Indonésia, agrupados pelas características silviculturais e botânicas. A população base constituiu-se de um banco clonal, com 6 anos de idade, implantado no Horto Florestal Nossa Senhora Aparecida,

Osmar Francisco Ruy; Mário Ferreira; Mário Tomazello Filho


Estudo de não gaussianidade nas anisotropias da RCF medidas Wmap  

NASA Astrophysics Data System (ADS)

A investigação do campo de flutuações da Radiação Cósmica de Fundo (RCF) pode oferecer um importante teste para os modelos cosmológicos que descrevem a origem e a evolução das flutuações primordiais. De um lado, apresenta-se o modelo inflacionário que prevê um espectro de flutuações adiabáticas distribuídas segundo uma gaussiana e, de outro, os modelos de defeitos topológicos (dentre outros) que descrevem um mecanismo para a geração de flutuações de isocurvatura que obedecem a uma distribuição não gaussiana. Este trabalho tem como objetivo caracterizar traços do modelo não gaussiano de campo misto (entre flutuações adiabáticas e de isocurvatura) nos mapas do Wilkinson Microwave Anisotropy Probe (WMAP). Simulações das anisotropias da RCF no contexto de mistura indicam traços marcantes na distribuição das flutuações de temperatura, mesmo quando consideradas pequenas contribuições do campo de isocurvatura (da ordem de 0.001). O efeito da mistura entre os campos resulta na transferência de potência de flutuações em escalas angulares intermediárias para flutuações em pequenas escalas angulares. Este efeito pode ser caracterizado pela relação entre as amplitudes dos primeiros picos acústicos no espectro de potência da RCF. Neste trabalho, investigamos a contribuição do campo de isocurvatura, no contexto de mistura, sobre as observações recentes da RCF realizadas pelo WMAP. As previsões do modelo de campo misto, uma vez confrontadas com as observações em pequenas escalas angulares, podem ajudar a revelar a natureza das flutuações primordiais.

Andrade, A. P. A.; Wuensche, C. A.; Ribeiro, A. L. B.



Hasty?yurv?da--a complete treatise on elephants.  


Ayurv?da, the oldest existing medical science of India, which is not only responsible for the health of human beings but also, plays an important in Veterinary sciences. In India, history of traditional Veterinary medicine dates back to the era of Mah?bh?rata i.e.5000 B.C., recorded in the form of "Nakula Samhhit?". Hast?yurv?da is a treatise on elephants, Palk?pya wrote this Samhit?. The present book is available as a complete Samhit?, edited by Pandit Shivadutta Sharma. Hast?yurv?da was printed at Anand??rama press in 1894. Hast?yurv?da has 160 Adhy?y?s. This Samhit? is based on fundamental principles of Ayurv?da, containing all aspects of Sharir including Anatomy & Physiology, Rog Vigy?na, Surgery and Treatment of elephants as well as their care. It is the one and only complete printed Samhit? and rare book on Hast?yurv?da by Palk?pya. PMID:18175649

Sharma, Mahesh Chandra



Autoras de obras didáticas e livros para o ensino da leitura produzidos no Rio Grande do Sul: contribuições à história da alfabetização (1950-1970)1  

Microsoft Academic Search

Resumo: A História da Alfabetização tem se constituído um importante campo de pesquisa no Brasil nos últimos anos, enfatizando, entre outras coisas, métodos e processos de ensino da leitura e da escrita, materiais de alfabetização, com destaque para as cartilhas escolares, histórias e trajetórias de vida de professoras alfabetizadoras, etc. Na pesquisa que realizamos desde 2001, temos privilegiado a análise

Eliane Peres


Modelagem do vento e da fotosfera de AG Carinae  

NASA Astrophysics Data System (ADS)

A trajetória evolutiva das estrelas de alta massa depende fortemente de suas taxas de perda de massa. Apesar do rápido progresso no estudo destas estrelas, a taxa de perda de massa e outros parâmetros físicos básicos, como a temperatura superficial e a velocidade terminal do vento ainda não estão bem determinados. Isto ocorre devido à presença de ventos irregulares, rápidos e fortes ao redor destas estrelas, tornando a interpretação dos seus espectros uma tarefa difícil. Assim, a modelagem do vento e da fotosfera dessas estrelas está sendo cada vez mais usada para obter tais parâmetros a partir dos espectros. O aumento da taxa de perda de massa durante a fase LBV (Variáveis Luminosas Azuis), comparado com outros tipos de estrelas, tem sido atribuído a instabilidades do tipo S Doradus. Dispomos de uma base de dados espectroscópicos cobrindo 22 anos de observações de AG Carinae, incluindo um ciclo S Doradus completo, com espectros CCD em alta resolução na faixa óptica e infravermelha. Utilizamos o programa desenvolvido por Schmutz (1997) para uma análise preliminar desse ciclo, obtendo a taxa de perda de massa a partir da linha do Ha. Não existe uma correlação clara da taxa de perda de massa com mudanças da temperatura efetiva, do raio da estrela e do fluxo na banda V. A estrela atingiu seu mínimo fotométrico (raio mínimo) em 1990 e o máximo fotométrico (raio máximo) em 1995, enquanto que o fluxo máximo da linha do Ha ocorreu em 1996. Além disso a taxa de perda de massa não segue esse ciclo, contrariamente às idéias correntes. Para fazer um modelo mais realista estamos usando o programa CMFGEN (Hillier & Miller), que trata a fotosfera e o vento estelar de forma consistente, considerando a radiação fora do equilíbrio termodinâmico (NLTE) e com blanketting total de linhas. Simulamos o espectro de AG Carinae em duas épocas extremas do ciclo S Dor para testar os resultados obtidos com o modelo mais simplificado.

Groh, J. H.; Damineli, A.



The Composition and Evolution of Extremely Hot DA White Dwarfs  

NASA Astrophysics Data System (ADS)

We propose high S/N {15:1 to 30:1} STIS far UV observations of the hottest known DA white dwarfs. With temperatures in the range 70, 000-130, 000K, these stars represent an important but little understood link between the H-rich central stars of planetary nebulae {CPN} and the general population of DA white dwarfs. Stellar temperatures are extremely difficult to determine in this range and depend upon knowledge of the photospheric composition, which is also a mystery. Consequently, their relationship to the cooler DA stars remains undefined. Far UV measurements of the abundances of heavy elements are essential for an ultimate determination of the stellar temperature. Coupling STIS data with an analysis of the Lyman lines obtained with planned FUSE observations, it will be possible to determine the physical parameters of these objects and finally track their evolution along the DA cooling sequence. In addition, we will search for evidence of mass outflow, in the form of blueward asymmetries in the heavy element line profiles, which is believed to cause departures from the compositions predicted by radiative levitation theory but which has been observed in only two DA white dwarfs.

Barstow, Martin



The ATLAS PanDA Monitoring System and its Evolution  

NASA Astrophysics Data System (ADS)

The PanDA (Production and Distributed Analysis) Workload Management System is used for ATLAS distributed production and analysis worldwide. The needs of ATLAS global computing imposed challenging requirements on the design of PanDA in areas such as scalability, robustness, automation, diagnostics, and usability for both production shifters and analysis users. Through a system-wide job database, the PanDA monitor provides a comprehensive and coherent view of the system and job execution, from high level summaries to detailed drill-down job diagnostics. It is (like the rest of PanDA) an Apache-based Python application backed by Oracle. The presentation layer is HTML code generated on the fly in the Python application which is also responsible for managing database queries. However, this approach is lacking in user interface flexibility, simplicity of communication with external systems, and ease of maintenance. A decision was therefore made to migrate the PanDA monitor server to Django Web Application Framework and apply JSON/AJAX technology in the browser front end. This allows us to greatly reduce the amount of application code, separate data preparation from presentation, leverage open source for tools such as authentication and authorization mechanisms, and provide a richer and more dynamic user experience. We describe our approach, design and initial experience with the migration process.

Klimentov, A.; Nevski, P.; Potekhin, M.; Wenaus, T.



1991 DA: An asteroid in a bizarre orbit  

NASA Technical Reports Server (NTRS)

Asteroidal object 1991 DA has an orbit of high inclination, crossing the planets from Mars to Uranus. This is unique for an asteroid, but not unusual for a comet of the Halley-type: it therefore seems likely that 1991 DA is an extinct or dormant comet. Previous CCD imaging has shown no indication of a coma; spectroscopic observations of 1991 DA which lack any evidence of strong comet-like emissions are reported. Numerical integrations of the orbit of this object were performed which show that is has been remarkably stable for the past approximately 20,000 yr, but chaotic before that. This may allow a new estimate to be made of the physical lifetimes of comets.

Steel, Duncan; Mcnaught, Robert H.; Asher, David



Geomagnetic observations on tristan da cunha, south atlantic ocean  

USGS Publications Warehouse

Few geomagnetic ground observations exist of the Earth's strongest core field anomaly, the South Atlantic Anomaly (SAA). The geomagnetic repeat station on the island Tristan da Cunha, located half-way between South Africa and South America at 37?? 05' S, 12?? 18' W, is therefore of crucial importance. We have conducted several sets of repeat station measurements during magnetically quiet conditions (Kp 2o or less) in 2004. The procedures are described and the results are compared to those from earlier campaigns and to the predictions of various global field models. Features of the local crustal bias field and the solar quiet daily variation are discussed. We also evaluate the benefit of continuous magnetic field recordings from Tristan da Cunha, and argue that such a data set is a very valuable addition to geomagnetic satellite data. Recently, funds were set up to establish and operate a magnetometer station on Tristan da Cunha during the Swarm magnetic satellite mission (2011-2014).

Matzka, J.; Olsen, N.; Maule, C. F.; Pedersen, L. W.; Berarducci, A. M.; Macmillan, S.



The Potential da Vinci in All of Us  

NSDL National Science Digital Library

The study of the human form is fundamental to both science and art curricula. For vertebrates, perhaps no feature is more important than the skeleton to determine observable form and function. As Leonard da Vinci's famous Proportions of the Human Figure (Virtruvian Man) illustrates, the size, shape, and proportions of the human body are defined by bones and their articulations. In this unit that focuses on the human skeleton, students are introduced to these concepts by asking them both to study da Vinci's drawing and build their own model of the human skeleton. Units on animal camouflage and architecture are also presented to further support the art and science connection.

Petto, Andrew; Petto, Sarah



The DaCHS Multi-protocol VO Server  

NASA Astrophysics Data System (ADS)

GAVO's Data Center Helper Suite (DaCHS) is a suite of tools for publishing data to the Virtual Observatory. It implements all major VO protocols (SCS, SIAP, SSAP, TAP, OAI-PMH). The integrated management and ingestion component allow defining metadata, structure, and services once and re-use the definition throughout the publication cycle from initial metadata aquisition to registry record generation. It has been driving GAVO's data center since 2008 and is now deployed in multiple locations around the globe. This poster briefly describes the design of the system as well as a bird's-eye view of data publishing with DaCHS.

Demleitner, M.



Mapeamento DA Vegetacao/USO DA Terra de Areas Indigenas Utilisando-Se Dados TM/LANDSAT (Mapping of Vegetation/Land Use of Indigenous Areas Utilizing TM/LANDSAT Data).  

National Technical Information Service (NTIS)

The objective of this work is to provide environmental information about indigenous areas (Ananas Ponta da Serra, Cajueiro, Ouro, Araca, Serra da Moca, Recanto da Saudade). The study area is located in Boa Vista, Bonfim, and Alto Alegre municipalities in ...

P. Hernandezfilho K. Defaria



A Run-time Environment for Da CaPo  

Microsoft Academic Search

It is a well known fact that the performance bottleneck of modern high speed networks is located in the end systems. Reasons are the complexity of the applied protocols and the embedding of protocols in the end systems. Da CaPo (D ynamic C onfigur ation of Pr otocols) provides an environment which allows the dynamic configuration of protocols with respect

M. Vogt; Bernhard Plattner; Thomas Plagemann; Thomas Walter




Microsoft Academic Search

As Reservas Extrativistas (RESEX) são áreas de domínio público, com uso concedido às populações extrativistas tradicionais, cuja subsistência baseia-se no extrativismo e na agricultura de subsistência e na criação de animais de pequeno porte, e têm como objetivos básicos proteger os meios de vida e a cultura dessas populações, e assegurar o uso sustentável dos recursos naturais da unidade. Porém,

Francisco Carlos Da Silveira Cavalcanti; Raimundo Claudio Gomes Maciel; Joao Alfredo De Carvalho Mangabeira; Bastiaan Philip Reydon




Microsoft Academic Search

o \\/h) e as matrizes de espécies por estação foram submetidas à análise de agrupamento e ordenação das amostras com base no índice de dissimilaridade de Bray-Curtis. Os dados indicaram a formação de distintas assembléias de espécies que representaram as diferentes regiões do talude ao longo da área de estudo. Variações significativas foram observadas entre médias e amplitudes de profundidade,



40 CFR 60.47Da - Commercial demonstration permit.  

Code of Federal Regulations, 2013 CFR

...Performance for Electric Utility Steam Generating Units § 60.47Da...for each 24-hour period of steam generator operation and to...following equivalent MW electrical generation capacity for any one equivalent MW electrical generation capacity for all...



How Computers shape Educational Activities at Casa da Música  

Microsoft Academic Search

In this paper we present the main ideas that shape musical experiences in the context of the Education Service program at Casa da Música, Porto and analyze how computers, applications and technology in general are part of a strategy that aims to provide a broad access to music making. A particular emphasis is given to technologies that have been purposely

P. M. Rodrigues; F. Lopes; A. P. Almeida; N. Peixoto; M. Mónica; J. Branco; P. J. Neto


Síndrome da cabeça caída em doença do neurônio motor  

Microsoft Academic Search

RESUMO - A síndrome da cabeça caída é causada por diminuição de força nos músculos extensores do pescoço sendo encontrada em diversas doenças neuro m u s c u l a res, bem como, na esclerose lateral amiotrófica. D e s c revemos o caso de três mulheres com diagnóstico de doença do neurônio motor com quadro clínico de disfagia

Paulo José Lorenzoni; Marcos Christiano Lange; Cláudia S. K. Kay; Luiz G. M. P. de Almeida; Hélio A. G. Teive; Rosana H. Scola; Lineu C. Werneck



Studying and Working Abroad. Leonardo da Vinci Series: Good Practices.  

ERIC Educational Resources Information Center

This document profiles recent successful examples of students studying and working abroad as part of the European Commission's Leonardo da Vinci program, which is designed to give students across the European Union the opportunity to experience vocational training in a foreign country. The following examples are presented: (1) 3 Finnish students…

Commission of the European Communities, Brussels (Belgium). Directorate-General for Education and Culture.


MICROPROPAGAÇÃO DA AROEIRA (Myracrodruon urundeuva Fr. All) 1  

Microsoft Academic Search

RESUMO - A aroeira (Myracrodruon urundeuva Fr. All) é uma Anacardiaceae lenhosa que tem sido usada de forma predatória pela qualidade de sua madeira e pelo uso med i- cinal de sua casca desprovida de súber, por meio da qual se fabrica antiinflamatório. O objetivo deste trabalho foi o e s- tabelecimento \\



Vozes católicas no Congresso Nacional: aborto, defesa da vida  

Microsoft Academic Search

Este levantamento sobre os argumentos apresentados nos debates do Congresso Nacional faz parte de um trabalho mais amplo sobre a tramitação de projetos de lei relativos aos direitos sexuais e reprodutivos que tramitaram na década de 90. O aborto, um dos eventos da vida reprodutiva das mulheres, aparece na pauta dos debates mais polêmicos das últimas décadas, tanto em nível

Myriam Aldana




Microsoft Academic Search

Resumo No presente estudo foram aplicados os conceitos de Produção Mais Limpa no setor da serraria e de beneficiamento em uma madeireira, com o intuito de contribuir para a redução dos desperdícios de matéria-prima , água e energia; visando a integração deste setor industrial nos conceitos de Ecologia Industrial. Foi elaborado o seguinte objetivo geral: desenvolver uma proposta para a

Thompson Copperfield von Agner; Carlos Cezar Stadler


A fauna apícola do Parque Municipal da Cachoeirinha (Iporá, GO)  

Microsoft Academic Search

Bees are dependent on flower nectar and pollen (main sources of protein and energy, respectively) and have an important economic and ecological role as pollinators, representing about 40 to 90% of phanerogams pollinators in several ecosystems. This work presents a description of bee species from Parque Municipal da Cachoeirinha in Iporá, Goiás state, Brazil. A total of 58 hours of

Leandro Rodrigues Santiago; Rute Magalhães Brito; Thiago Mahlmann Vitoriano Lopes Muniz; Favízia Freitas de Oliveira; Flávio de Oliveira Francisco



A 16-bit 4'th order noise-shaping D\\/A converter  

Microsoft Academic Search

A 16-bit oversampling D\\/A (digital-to-analog) converter has been designed using a fourth-order all-digital noise-shaping loop followed by a 3-bit D\\/A converter. The 3-bit D\\/A converter, which uses a novel form of dynamic element matching achieves high accuracy and long-term stability without requiring precision matching of components. The harmonic distortion of the untrimmed monolithic CMOS prototype D\\/A converter is less than

L. Richard Carley; John Kenney



Yarkovsky-driven Impact Predictions: Apophis and 1950 DA  

NASA Astrophysics Data System (ADS)

Orbit determination for Near-Earth Asteroids presents unique technical challenges due to the imperative of early detection and careful assessment of the risk posed by specific Earth close approaches. The occurrence of an Earth impact can be decisively driven by the Yarkovsky effect, which is the most important nongravitational perturbation as it causes asteroids to undergo a secular variation in semimajor axis resulting in a quadratic effect in anomaly. We discuss the cases of (99942) Apophis and (29075) 1950 DA. The relevance of the Yarkovsky effect for Apophis is due to a scattering close approach in 2029 with minimum geocentric distance ~38000 km. For 1950 DA the influence of the Yarkovsky effect in 2880 is due to the long time interval preceding the impact. We use the available information from the astrometry and the asteroids' physical models and dynamical evolution as a starting point for a Monte Carlo method that allows us to measure how the Yarkovsky effect affects orbital predictions. We also find that 1950 DA has a 98% likelihood of being a retrograde rotator. For Apophis we map onto the 2029 close approach b-plane and analyze the keyholes corresponding to resonant close approaches. For 1950 DA we use the b-plane corresponding to the possible impact in 2880. We finally compute the impact probability from the mapped probability density function on the considered b-plane. For Apophis we have 4 in a million chances of an impact in 2068, while the probability of Earth impact in 2880 for 1950 DA is 0.04%.

Chesley, Steven R.; Farnocchia, D.; Chodas, P. W.; Milani, A.




Microsoft Academic Search

Resumo: Este artigo apresenta resultados de previsão da vazão afluente mensal do Rio São Francisco, que alimenta a usina de Sobradinho, usando-se redes neurais de Elman (RNA). As redes neurais de Elman utilizadas foram preparadas com o objetivo de receber como entrada amostras da série temporal da vazão afluente do Rio São Francisco deslocadas mês a mês. Para tal, nas

Luiz Biondi Neto; Pedro Henrique Gouvêa Coelho; Luís Chiganer; Lídia Angulo Meza; Luiz Palmeira Monteiro



First experiences with the da Vinci™ operating robot in thoracic surgery  

Microsoft Academic Search

Objectives: The da Vinci™ surgical robotic system was purchased at our institution in June 2001. The aim of this trial was to evaluate the applicability of the da Vinci™ operation robot for general thoracic procedures. Methods: The da Vinci™ surgical system consists of a console connected to a surgical arm cart, a manipulator unit with two instrument arms and a

J. Bodner; H. Wykypiel; G. Wetscher; T. Schmid



40 CFR 60.42Da - Standards for particulate matter (PM).  

Code of Federal Regulations, 2013 CFR

...false Standards for particulate matter (PM). 60.42Da Section 60.42Da Protection...42Da Standards for particulate matter (PM). (a) Except as provided in...March 1, 2005, any gases that contain PM in excess of 13 ng/J (0.03...



Impacto social da produção integrada de abacaxi no estado do Tocantins – Brasil  

Microsoft Academic Search

Além do objeto da produção em si, a produção integrada tem como princípios o bem-estar social e a preservação ambiental. Neste artigo avaliam-se os impactos sociais da produção integrada de abacaxi no Estado do Tocantins (Brasil) em oito estabelecimentos rurais. A ferramenta empregada na avaliação, denominada Ambitec Social, foi desenvolvida pela Embrapa, sob a liderança da Embrapa Meio Ambiente. O

Clovis Oliveira de Almeida; Aristoteles Pires de Matos; Carlos Estevao Leite Cardoso



Os determinantes da desigualdade de renda no Brasil: Luta de classes ou heterogeneidade educacional?  

Microsoft Academic Search

Este trabalho considera a questão: quais os principais determinantes da desigualdade de renda no Brasil? Após lembrar a importância da questão para a nossa economia, o artigo sugere um arcabouço genérico para categorizar os determinantes da distribuição de renda, baseado em cinco grupos de fatores: caraterísticas natas dos indivíduos; suas características adquiridas; o papel do mercado de trabalho; o papel

Francisco de Hollanda Guimarães Ferreira




Microsoft Academic Search

1 - INTRODUÇÃO A densidade da madeira é uma característica complexa resultante da combinação de diversos fatôres. Existem inúmeros trabalhos mostrando sua relação com as dimensões das fibras, particularmente espessura da parede, volume dos vasos e parênquimas, proporção entre madeira primaveril e outonal, e arranjo dos elementos anatômicos. Êste assunto tem sido intensivamente estudado para as coníferas, porém para as

Celso Edmundo; Bochetti Foelkel; Maria Aparecida Mourão Brasil; Luiz Ernesto; George Barrichelo


Application of DA-Preconditioned FINN for Electric Power System Fault Detection  

Microsoft Academic Search

This paper proposes a hybrid method of Deterministic Annealing (DA) and Fuzzy Inference Neural Network (FINN) for electric power system fault detection. It extracts features of input data with two-staged precondition of Fast Fourier Transform (FFT) and DA. FFT is useful for extracting the features of fault currents while DA plays a key role to classify input data into clusters

Tadahiro Itagaki; Hiroyuki Mori; Takeshi Yamada; Shoichi Urano



Novos Aspectos da Regulação do Trabalho no Brasil. Qual o Papel do Estado?  

Microsoft Academic Search

Uma inserção civilizada dos trabalhadores nos âmbitos econômico, social e político da vida brasileira depende da existência de relações assalariadas com direitos trabalhistas e previdenciários assegurados. Não obstante, ao longo dos anos 1990 e em parte dos atuais, os trabalhadores tenderam a assumir outras posições na estrutura de ocupações – posições quase sempre distintas da de assalariados formalmente registrados. Em

André Gambier Campos




Microsoft Academic Search

TABAI, K. C.; SALAY, E.; BARBOSA, C. G. Effects of a program for the analysis of food quality on consumer in the city of Campinas, São Paulo, Brazil. Revista de Ciências da Vida, Seropédica, RJ: EDUR, v. 29, n. 1, jan. - jun., p. 14-26, 2009. The Brazilian National Institute of Metrology & Industrial Quality (INMETRO) carries out a program




Microsoft Academic Search

They will be presented resulted gotten from the exe cuted digital mapping in the arenaceous pontal and in the dune field located in the beach of Atafona, São João da Barra (RJ), from geodesic surveys with GPS, in the ways relative and kinematic, with use of GTR-A\\/TechGeo dredges. Ahead of the ambient problem installed in this litt oral zone, next



Crescimento ponderal de filhotes de tartaruga gigante da Amazônia (Podocnemis expansa) submetidos a tratamento com rações isocalóricas contendo diferentes níveis de proteína bruta  

Microsoft Academic Search

RESUMO - A criação de Podocnemis expansa, a tartaruga-da-amazônia, com finalidade comercial foi implantada no Brasil em 1992. Contudo, muitas questões acerca do manejo, da nutrição, da sanidade e, principalmente, da reprodução ainda são obscuras. Visando contribuir para o desenvolvimento de uma metodologia alimentar da espécie, objetivou-se testar diferentes níveis de proteína da dieta. Uma amostra de 480 animais neonatos

Vinicius Augusto Sá; Leonardo C. Quintanilha; Gustavo Eduardo Freneau; Vera Lucia Ferreira Luz; Arcádio de Los Reyes Borja; Paulo César Silva



JCoDA: a tool for detecting evolutionary selection  

PubMed Central

Background The incorporation of annotated sequence information from multiple related species in commonly used databases (Ensembl, Flybase, Saccharomyces Genome Database, Wormbase, etc.) has increased dramatically over the last few years. This influx of information has provided a considerable amount of raw material for evaluation of evolutionary relationships. To aid in the process, we have developed JCoDA (Java Codon Delimited Alignment) as a simple-to-use visualization tool for the detection of site specific and regional positive/negative evolutionary selection amongst homologous coding sequences. Results JCoDA accepts user-inputted unaligned or pre-aligned coding sequences, performs a codon-delimited alignment using ClustalW, and determines the dN/dS calculations using PAML (Phylogenetic Analysis Using Maximum Likelihood, yn00 and codeml) in order to identify regions and sites under evolutionary selection. The JCoDA package includes a graphical interface for Phylip (Phylogeny Inference Package) to generate phylogenetic trees, manages formatting of all required file types, and streamlines passage of information between underlying programs. The raw data are output to user configurable graphs with sliding window options for straightforward visualization of pairwise or gene family comparisons. Additionally, codon-delimited alignments are output in a variety of common formats and all dN/dS calculations can be output in comma-separated value (CSV) format for downstream analysis. To illustrate the types of analyses that are facilitated by JCoDA, we have taken advantage of the well studied sex determination pathway in nematodes as well as the extensive sequence information available to identify genes under positive selection, examples of regional positive selection, and differences in selection based on the role of genes in the sex determination pathway. Conclusions JCoDA is a configurable, open source, user-friendly visualization tool for performing evolutionary analysis on homologous coding sequences. JCoDA can be used to rapidly screen for genes and regions of genes under selection using PAML. It can be freely downloaded at



A Device Enhancement for the Dry Sliding Friction Coefficient Measurement Between Steel 1080 and VascoMax with Respect to Surface Roughness Changes  

Microsoft Academic Search

An enhancement of an existing tribometer device developed by Philippon et al. (Wear 257:777–784, 2004) is presented in this work. This experimental device is made up of a dynamometer ring and a specific load sensor allowing\\u000a to apply an apparent normal force on specimens and to measure frictional forces respectively. A set of strain gauges are added\\u000a to the upgraded

S. Philippon; G. Z. Voyiadjis; L. Faure; A. Lodygowski; A. Rusinek; P. Chevrier; E. Dossou



[Typology of nosocomial architecture on both sides of the Atlantic Ocean, XVI century. Enrique Egas' model in Spain and Vasco de Quiroga's model in New Spain].  


Two visions of the nosocomial architecture are discussed, located in a close time period, 1505-1535, but inserted in two different scenarios. One is in the Renaissance Spain, proposed by the architect E. Egas (born in Toledo, Spain), and the other one in the New Spain, proposed by V. de Quiroga, who chose an architectural style coherent with the Franciscan ideals of humbleness and evangelization, which set aside the ornamentation typical of Spanish medieval hospitals rather than palatial monuments built by E. Egas. The "hospital-village" project by V. de Quiroga allowed the patients and their families to live together, which was accepted by pre-Hispanic families that in the time were extensive. The hospital-village, both in its typology and in its health conception, returns to designs already in disuse of the Spanish Middle Age by picking up the idea of a hospital as a multifunctional space in which sanitary attention, nurseries, and shelters for poor people were combined within the church. PMID:23999639

Rodríguez-Orozco, Alain Raimundo; Rodríguez Pérez, Martha Eugenia



The isolated massive DA white dwarf GD 50  

NASA Astrophysics Data System (ADS)

Analysis of accurate hydrogen line profiles from optical and ultraviolet spectrophotometry shows that the hot DA white dwarf GD 50 (WD 0346-011) has an exceptionally high surface gravity of log g = 9.0 + or - 0.15; the derived parameters imply a mass of 1.2 solar mass and a radius of 0.0057 solar mass, if an interior composed of carbon and oxygen is assumed. As such, it is the first well-studied, isolated DA white dwarf with a likely mass larger than that of Sirius B. Moreover, the derived mass is large enough to consider the possibility that the interior is composed of oxygen, neon, and magnesium. If GD 50 has evolved as a single object, it should be quite young. Alternatively, the star could have formed as the result of a merger of a close pair of white dwarfs.

Bergeron, P.; Kidder, K. M.; Holberg, J. B.; Liebert, James; Wesemael, F.; Saffer, Rex A.



The Da Vinci code dynamically de-coded.  


The novel The Da Vinci Code, by Dan Brown has been on best-seller lists for over two years. An examination of Brown's previous novels reveals a well-designed plot line shared by all four novels that not only makes them good "thrillers" but also creates a mythological structure to the novels that draws on common unconscious fantasies in the same way that fairy tales do. One aspect of this mythological structure is the use of evil conspiracies (and benign ones as well) for the protagonist to overcome. In addition, The Da Vinci Code presents a religious theme involving legends about Mary Magdalene. This theme touches on the role of a feminine aspect to divinity in allowing for an erotic connection with the divine. PMID:16448349

Cohen, Mariam




Microsoft Academic Search

1 RESUMO ñ Este trabalho teve por objetivo avaliar o desempenho de mudas da espØcie angico-branco (Anadenanthera colubrina (Vell.) Brenan), em condiÁies diferenciadas de disponibilidade de fÛsforo no solo e de saturaÁªo por bases. Os tratamentos foram representados por um fatorial de seis nÌveis de P (0, 100, 200, 300, 400 e 500 mg\\/dm 3 ) por cinco nÌveis de

Cristina de Oliveira Gomes; Haroldo Nogueira de Paiva; Lima Neves; Ricardo Silva



Improving Security in the ATLAS PanDA System  

NASA Astrophysics Data System (ADS)

The security challenges faced by users of the grid are considerably different to those faced in previous environments. The adoption of pilot jobs systems by LHC experiments has mitigated many of the problems associated with the inhomogeneities found on the grid and has greatly improved job reliability; however, pilot jobs systems themselves must then address many security issues, including the execution of multiple users' code under a common 'grid' identity. In this paper we describe the improvements and evolution of the security model in the ATLAS PanDA (Production and Distributed Analysis) system. We describe the security in the PanDA server which is in place to ensure that only authorized members of the VO are allowed to submit work into the system and that jobs are properly audited and monitored. We discuss the security in place between the pilot code itself and the PanDA server, ensuring that only properly authenticated workload is delivered to the pilot for execution. When the code to be executed is from a 'normal' ATLAS user, as opposed to the production system or other privileged actor, then the pilot may use an EGEE developed identity switching tool called gLExec. This changes the grid proxy available to the job and also switches the UNIX user identity to protect the privileges of the pilot code proxy. We describe the problems in using this system and how they are overcome. Finally, we discuss security drills which have been run using PanDA and show how these improved our operational security procedures.

Caballero, J.; Maeno, T.; Nilsson, P.; Stewart, G.; Potekhin, M.; Wenaus, T.




Microsoft Academic Search

RESUMO - Foi avaliada a ocorrŒncia e a distribuiÁªo de espØcies de fungos micorrÌzicos arbusculares (FMAs) e Acetobacter diazotrophicus em plantios de cana-de-aÁœcar em diferentes tipos de manejo nos Estados do Rio de Janeiro e Pernambuco. Foram feitas 35 coletas de amostras de solo da rizosfera e de raÌzes de 14 variedades de cana-de-aÁœcar para extraÁªo de esporos e isolamento



DaRT: A CALL System to Help Students Practice and Develop Reasoning Skills in Choosing English Articles.  

ERIC Educational Resources Information Center

Describes DaRT, a computer assisted language-learning system for helping English-as-a-Second-Language students master English articles. DaRT uses a diagrammatic reasoning tool to present communicative contexts for exercises in choosing appropriate articles. This paper describes the development of DaRT and DaRT's system components and concludes…

Yoshii, Rika; Milne, Alastair



Análise da medição do raio solar em ultravioleta  

NASA Astrophysics Data System (ADS)

A medição acurada do raio solar em qualquer banda do espectro eletromagnético é de relevância na formulação e calibração de modelos da estrutura e atmosfera solar. Esses modelos atribuem emissão do contínuo do Sol calmo em microondas à mesma região da linha Ha do Hell. Apresentamos a medição do raio solar em UV com imagens do EIT (Extreme Ultraviolet Image Telescope) entre 1996 e 2002, no comprimento de onda 30,9 nm (Ha do Hell), que se forma na região de transição/cromosfera solar. A técnica utilizada para o cálculo do raio UV foi baseada na transformada Wavelet B3spline. Fizemos um banco de dados com 1 imagem por dia durante o período citado. Obtivemos como resultado o raio médio da ordem de 975.61" e uma diminuição do mesmo para o período citado variando em média -0,45" /ano. Comparamos estes dados com os valores obtidos pelo ROI (Radio Observatório de Itapetinga) em 22/48 GHz e Nobeyama Radio Heliograph em 17 GHz mostrando que os raios médios são muito próximos o que indica que a região de formação nessas freqüências é a mesma conforme os modelos. Comparamos os resultados também com outros índices de atividade solar.

Saraiva, A. C. V.; Giménez de Castro, C. G.; Costa, J. E. R.; Selhorst, C. L.; Simões, P. J. A.



Some radical queries.  


A little over 500 years ago, Vasco da Gama arrived on the west coast of India after the first direct sea voyage from Portugal with the intention of developing trade in spices, particularly in black pepper. Portugese took the merchandise, and much more; they ended up colonizing Goa. In 1999 delegates from all over the world came to this fabulous Goa for a symposium on antioxidants, some of which are natural products found in spices. Some views and queries on the interaction of antioxidants and radicals are presented. PMID:10962126

Ramasarma, T



The 29-kDa proteins phosphorylated ion thrombin-activated human platelets are forms of the estrogen receptor-related 27-kDa heat shock protein  

SciTech Connect

Thrombin plays a critical role in platelet activation, hemostasis, and thrombosis. Cellular activation by thrombin leads to the phosphorylation of multiple proteins, most of which are unidentified. The authors have characterized several 29-kDa proteins that are rapidly phosphorylated following exposure of intact human platelets to thrombin. A murine monoclonal antibody raised to an unidentified estrogen receptor-related 29-kDa protein selectively recognized these proteins as well as a more basic, unphosphorylated 27-kDa protein. Cellular activation by thrombin led to a marked shift in the proportion of protein from the 27-kDa unphosphorylated form to the 29-kDa phosphoprotein species. Using this antibody, they isolated and sequenced a human cDNA clone encoding a protein that was identical to the mammalian 27-kDa heat shock protein (HSP27), a protein of uncertain function that is known to be phosphorylated to several forms and to be transcriptionally induced by estrogen. The 29-kDa proteins were confirmed to be phosphorylated forms of HSP27 by immunoprecipitation studies. Thus, the estrogen receptor-related protein is HSP27, and the three major 20-kDa proteins phosphorylated in thrombin-activated platelets are forms of HSP27. These data suggest a role for HSP27 in the signal transduction events of platelet activation.

Mendelsohn, M.E.; Yan Zhu; O'Neill, S. (Harvard Medical School, Boston, MA (United States))



Métodos de coleta de fezes e determinação dos coeficientes de digestibilidade da fração protéica e da energia de alimentos para o pacu, Piaractus mesopotamicus (Holmberg, 1887)  

Microsoft Academic Search

RESUMO - Para melhorar a alimentação do pacu (Piaracatus mesopotamicus), é necessário conhecer o nível de aproveitamento dos ingredientes utilizados em suas dietas. Neste trabalho, inicialmente, foram analisados quatro métodos de coleta de fezes, em função da precisão e da versatilidade (dissecação, extrusão, Guelph e Guelph modificado), e o tempo entre as coletas de fezes nos sistemas de Guelph, por

Eduardo Gianini Abimorad; Dalton José Carneiro



Purification and functional characterization of the 112 kDa adhesin of Entamoeba histolytica.  


The 112 kDa adhesin of E. histolytica is directly involved in the cytopathogenic activity of the parasite. We describe here the purification of the 112 kDa protein by electroelution and immunoaffinity chromatography using a monoclonal antibody against the adhesin. Two proteins of 70 and 50 kDa were eluted from the immunoaffinity column along with the 112 kDa adhesin. The three proteins were recognized by monospecific polyclonal antibodies against the adhesin. The same peptides (72 and 56 kDa) were also observed after incubation of the purified intact adhesin in diethylamine buffer. Proteins of 112 and 72 kDa were found to have protease activity, evidenced by their ability to degrade gelatin. Our results indicate that the 112 kDa adhesin was specifically broken down into two polypeptides of 50-56 and 70-72 kDa. The significance of this in vivo is as yet unclear. The adhesin has proteolytic activity, which is retained in the 70-72 kDa polypeptide but not in the 50-56 kDa one. PMID:1340303

Rigothier, M C; García-Rivera, G; Guaderrama, M; Orozco, E



Respostas religiosas ? aids no Brasil: impress?es de pesquisa acerca da Pastoral de DST/Aids da Igreja Cat?lica1  

PubMed Central

O texto encontra-se estruturado em quatro partes. Na primeira delas, apresentamos um conjunto de considerações e informações acerca da situação da aids no Brasil, das relações entre religião, sexualidade, aids e estado laico, bem como uma descrição mais clara do Projeto Respostas Religiosas ao HIV/Aids no Brasil, do qual este texto apresenta algumas impressões de pesquisa preliminares. A seguir, dedicamos um item a apresentação da Pastoral de DST/Aids, sua história, estrutura e objetivos. No item seguinte problematizamos diversas questões em particular no âmbito das relações Estado e Igreja, relações entre agentes de pastoral e hierarquia da Igreja, e questões ligadas mais diretamente à sexualidade e aids, todas referenciadas ao trabalho da Pastoral de DST/Aids. Ao final, apresentamos a bibliografia e fontes consultadas.

Seffner, Fernando; Silva, Cristiane Goncalves Meireles da; Maksud, Ivia; Garcia, Jonathan; Rios, Luis Felipe; Natividade, Marcelo; Borges, Priscila Rodrigues; Parker, Richard; Terto, Veriano



Evolução da morfologia de fases de blendas PA6\\/AES em extrusora de dupla rosca e moldagem por injeção  

Microsoft Academic Search

Resumo: A evolução da morfologia de fases em blendas não reativas e reativas de poliamida-6 com copolímero de acrilonitrila\\/EPDM\\/estireno (AES) em uma extrusora de rosca dupla co-rotacional foi investigada. A evolução da morfologia de fases ao longo da extrusora de rosca dupla foi monitorada através da coleta de pequenas amostras em válvulas no barril da extrusora e caracterização por microscopia

Adriane Bassani; Elias Hage Jr; Luiz A. Pessan; Ana V. Machado; José A. Covas



Surface Fatigue Life of High Temperature Gear Materials.  

National Technical Information Service (NTIS)

Three high temperature gear materials were evaluated using spur gear surface fatigue tests. These materials were, VASCO max 350, VASCO matrix 2, and nitralloy N and were evaluated for possible use in high temperature gear applications. The fatigue life of...

D. P. Townsend



Reconstitution of surfactant activity by using the 6 kDa apoprotein associated with pulmonary surfactant.  

PubMed Central

Lipid extracts of bovine pulmonary surfactant containing the 6 kDa apoprotein, but lacking the 35 kDa apoprotein, can mimic the essential characteristics of pulmonary surfactant on a pulsating-bubble surfactometer. Reconstituted surfactant can be produced by combining silicic acid fractions containing 6 kDa apoprotein and phosphatidylglycerol with phosphatidylcholine. Treatment of the protein-containing fraction with proteolytic enzymes abolishes its efficacy. These results indicate that the presence of the 6 kDa apoprotein can account for some of the essential physical and biological characteristics of pulmonary surfactant. Immunodiffusion studies indicate that, contrary to earlier suggestions, the 6 kDa apoprotein is not structurally related to the major surfactant apoprotein that has a molecular mass of 35 kDa. Images Fig. 2. Fig. 3. Fig. 4.

Yu, S H; Possmayer, F



Mulheres de corpo e alma: aspectos biopsicossociais da meia-idade feminina  

Microsoft Academic Search

Resumo Este trabalho investigou na literatura cientÌfica os aspectos biolÛgicos, psicolÛgicos e socioculturais que se estªo em jogo na etapa da meia-idade feminina a fim de melhor compreender a vivŒncia dessa mulher. O evento da menopausa ñ caracterizada pela cessaÁªo da ovulaÁªo e por manifestaÁies fÌsicas e psÌquicas - marca este momento vital e impie questies que, se nªo forem

Maria Elizabeth Mori; Vera Lucia Decnop Coelho



Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)  

PubMed Central

Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe Context-Dependent Assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA ZFNs, we rapidly altered 20 genes in zebrafish, Arabidopsis, and soybean. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multi-gene pathways or genome-wide alterations.

Sander, Jeffry D.; Dahlborg, Elizabeth J.; Goodwin, Mathew J.; Cade, Lindsay; Zhang, Feng; Cifuentes, Daniel; Curtin, Shaun J.; Blackburn, Jessica S.; Thibodeau-Beganny, Stacey; Qi, Yiping; Pierick, Christopher J.; Hoffman, Ellen; Maeder, Morgan L.; Khayter, Cyd; Reyon, Deepak; Dobbs, Drena; Langenau, David M.; Stupar, Robert M.; Giraldez, Antonio J.; Voytas, Daniel F.; Peterson, Randall T.; Yeh, Jing-Ruey J.; Joung, J. Keith



A 50-kDa variant form of human surfactant protein D  

Microsoft Academic Search

The dominant form of human surfactant protein D (SP-D) is a mul- timeric collagenous glycoprotein composed of monomeric subunits that have a molec- ular mass of 43 kDa under reducing conditions. However, in evaluating monoclonal antibodies to human SP-D, an additional monomeric subunit was identified with a reduced molecular mass of 50 kDa. This 50-kDa variant was detected in approximately

R. J. Mason; L. D. Nielsen; Y. Kuroki; E. Matsuura; J. H. Freed; J. M. Shannon



A 28-kDa cerebral neuropeptide from Manduca sexta: Relationship to the insect prothoracicotropic hormone  

Microsoft Academic Search

1.A 28-kDa peptide from the brain of the tobacco hornworm,Manduca sexta, was purifiedvia HPLC. The peptide copurified with the insect neurohormone, prothoracicotropic hormone (PTTH), through two HPLC columns.2.Immunocyctochemistry using polyclonal antibodies against the 28-kDa peptide revealed that the peptide was produced in the same protocerebral neurons that produce PTTH. Western blot analysis demonstrated that the 28-kDa peptide and big PTTH

Rosemary S. Gray; David P. Muehleisen; Eva J. Katahira; Walter E. Bollenbacher



Easy CD-DA Extractor 4.5.2  

NSDL National Science Digital Library

Billing itself as "the most complete CD-ripping solution available" as well as the fastest (a 40 percent increase in performance since the last edition), Easy CD-DA Extractor supports a variety of formats: mp3, Windows Media Player, Ogg Vorbis, TwinVQ, .wav, and .aiff. Using the program, you can copy CDs and convert from any of the supported formats (except .aiff) to any other. Users can define the length of the tracks they record; download in formation from the freedb, the Internet Compact Disc Database; normalize the music; and more. The program is shareware and costs $19.95.



Visual tracking of da Vinci instruments for laparoscopic surgery  

NASA Astrophysics Data System (ADS)

Intraoperative tracking of laparoscopic instruments is a prerequisite to realize further assistance functions. Since endoscopic images are always available, this sensor input can be used to localize the instruments without special devices or robot kinematics. In this paper, we present an image-based markerless 3D tracking of different da Vinci instruments in near real-time without an explicit model. The method is based on different visual cues to segment the instrument tip, calculates a tip point and uses a multiple object particle filter for tracking. The accuracy and robustness is evaluated with in vivo data.

Speidel, S.; Kuhn, E.; Bodenstedt, S.; Röhl, S.; Kenngott, H.; Müller-Stich, B.; Dillmann, R.



Chemical catalysis by the translocator protein (18 kDa).  


Translocator proteins (18 kDa) (TSPOs) are conserved integral membrane proteins. In both eukaryotes and prokaryotes, TSPOs interact with porphyrins, precursors of heme, and photosynthetic pigments. Here we demonstrate that bacterial TSPOs catalyze rapid porphyrin degradation in a light- and oxygen-dependent manner. The reaction is inhibited by a synthetic TSPO ligand PK11195 and by mutations of conserved residues, which affect either porphyrin binding or catalytic activity. We hypothesize that TSPOs are ancient enzymes mediating porphyrin catabolism with the consumption of reactive oxygen species. PMID:23651039

Ginter, Christopher; Kiburu, Irene; Boudker, Olga



Evolution of the ATLAS PanDA workload management system for exascale computational science  

NASA Astrophysics Data System (ADS)

An important foundation underlying the impressive success of data processing and analysis in the ATLAS experiment [1] at the LHC [2] is the Production and Distributed Analysis (PanDA) workload management system [3]. PanDA was designed specifically for ATLAS and proved to be highly successful in meeting all the distributed computing needs of the experiment. However, the core design of PanDA is not experiment specific. The PanDA workload management system is capable of meeting the needs of other data intensive scientific applications. Alpha-Magnetic Spectrometer [4], an astro-particle experiment on the International Space Station, and the Compact Muon Solenoid [5], an LHC experiment, have successfully evaluated PanDA and are pursuing its adoption. In this paper, a description of the new program of work to develop a generic version of PanDA will be given, as well as the progress in extending PanDA's capabilities to support supercomputers and clouds and to leverage intelligent networking. PanDA has demonstrated at a very large scale the value of automated dynamic brokering of diverse workloads across distributed computing resources. The next generation of PanDA will allow other data-intensive sciences and a wider exascale community employing a variety of computing platforms to benefit from ATLAS' experience and proven tools.

Maeno, T.; De, K.; Klimentov, A.; Nilsson, P.; Oleynik, D.; Panitkin, S.; Petrosyan, A.; Schovancova, J.; Vaniachine, A.; Wenaus, T.; Yu, D.; the Atlas Collaboration



SBS TDMA-DA system with VAC and DAC  

NASA Astrophysics Data System (ADS)

This paper describes many of the design considerations involved in developing the demand assignment (DA) subsystem of the TDMA satellite communication system for Satellite Business Systems. Each earth station has a satellite communications controller (SCC) which requests capacity to meet current voice and data traffic demands. A central reference station frequently reallocates capacity based on the requests of all the earth stations in a network. To minimize the amount of satellite capacity required, the SCC has a circuit-switching capability for voice calls as well as for digital data calls. Furthermore, the SCC employs voice activity compression (VAC) and data activity compression (DAC), in which the amount of capacity requested is based on measured average speech activity as well as the number of off-hook voice ports and the number of off-hook data ports. Data calls are queued on a first-come, first-served basis when capacity is not immediately available. The reference station distributes excess capacity according to a nonlinear table lookup procedure so that the voice call blocking probability is equalized across the network. The DA system makes much more efficient use of satellite transmission capacity than a design with fixed-capacity trunk routes.

Goode, B.



Metabonomics based NMR in Crohn's disease applying PLS-DA  

PubMed Central

Aim The aim of this study was to search for metabolic biomarkers of Crohn's disease (CD). Background Crohn's disease (CD) is a type of inflammatory bowel disease that causing a wide variety of symptoms. CD can influence any part of the gastrointestinal tract from mouth to anus. CD is not easily diagnosed because monitoring tools are currently insufficient. Thus, the discovery of proper methods is needed for early diagnosis of CD. Patients and methods We utilized metabolic profiling using proton nuclear magnetic resonance spectroscopy (1HNMR) to find the metabolites in serum. Classification of CD and healthy subject was done using partial least squares discriminant analysis (PLS-DA). Results According to PLS-DA model, we concluded that just using one descriptor CD and control groups could be classified separately. The level of lipid in blood serum of CD compared to healthy cohorts was decreased. For the external test set, the classification model showed a 94% correct classification of CD and healthy subject. Conclusion The result of classification model presents that NMR based metabonomics is key tool as well as insight into potential targets for disease therapy and prevention.

Fathi, Fariba; Oskouie, Afsaneh Arefi; Tafazzoli, Mohsen; Naderi, Nosratollah; Sohrabzedeh, Kaveh; Fathi, Soraya; Norouzinia, Mohsen



The 37kDa/67kDa Laminin Receptor acts as a receptor for A?42 internalization.  


Neuronal loss is a major neuropathological hallmark of Alzheimer's disease (AD). The associations between soluble A? oligomers and cellular components cause this neurotoxicity. The 37?kDa/67?kDa laminin receptor (LRP/LR) has recently been implicated in A? pathogenesis. In this study the mechanism underlying the pathological role of LRP/LR was elucidated. Försters Resonance Energy Transfer (FRET) revealed that LRP/LR and A? form a biologically relevant interaction. The ability of LRP/LR to form stable associations with endogenously shed A? was confirmed by pull down assays and A?-ELISAs. Antibody blockade of this association significantly lowered A?42 induced apoptosis. Furthermore, antibody blockade and shRNA mediated downregulation of LRP/LR significantly hampered A?42 internalization. These results suggest that LRP/LR is a receptor for A?42 internalization, mediating its endocytosis and contributing to the cytotoxicity of the neuropeptide by facilitating intra-cellular A?42 accumulation. These findings recommend anti-LRP/LR specific antibodies and shRNAs as potential therapeutic tools for AD treatment. PMID:24990253

Da Costa Dias, Bianca; Jovanovic, Katarina; Gonsalves, Danielle; Moodley, Kiashanee; Reusch, Uwe; Knackmuss, Stefan; Weinberg, Marc S; Little, Melvyn; Weiss, Stefan F T



The 37kDa/67kDa Laminin Receptor acts as a receptor for A?42 internalization  

PubMed Central

Neuronal loss is a major neuropathological hallmark of Alzheimer's disease (AD). The associations between soluble A? oligomers and cellular components cause this neurotoxicity. The 37?kDa/67?kDa laminin receptor (LRP/LR) has recently been implicated in A? pathogenesis. In this study the mechanism underlying the pathological role of LRP/LR was elucidated. Försters Resonance Energy Transfer (FRET) revealed that LRP/LR and A? form a biologically relevant interaction. The ability of LRP/LR to form stable associations with endogenously shed A? was confirmed by pull down assays and A?-ELISAs. Antibody blockade of this association significantly lowered A?42 induced apoptosis. Furthermore, antibody blockade and shRNA mediated downregulation of LRP/LR significantly hampered A?42 internalization. These results suggest that LRP/LR is a receptor for A?42 internalization, mediating its endocytosis and contributing to the cytotoxicity of the neuropeptide by facilitating intra-cellular A?42 accumulation. These findings recommend anti-LRP/LR specific antibodies and shRNAs as potential therapeutic tools for AD treatment.

Da Costa Dias, Bianca; Jovanovic, Katarina; Gonsalves, Danielle; Moodley, Kiashanee; Reusch, Uwe; Knackmuss, Stefan; Weinberg, Marc S.; Little, Melvyn; Weiss, Stefan F. T.



Identification of interaction domains of the prion protein with its 37-kDa/67-kDa laminin receptor  

PubMed Central

Cell-binding and internalization studies on neuronal and non-neuronal cells have demonstrated that the 37-kDa/67-kDa laminin receptor (LRP/LR) acts as the receptor for the cellular prion protein (PrP). Here we identify direct and heparan sulfate proteoglycan (HSPG)-dependent interaction sites mediating the binding of the cellular PrP to its receptor, which we demonstrated in vitro on recombinant proteins. Mapping analyses in the yeast two-hybrid system and cell-binding assays identified PrPLRPbd1 [amino acids (aa) 144–179] as a direct and PrPLRPbd2 (aa 53–93) as an indirect HSPG-dependent laminin receptor precursor (LRP)-binding site on PrP. The yeast two-hybrid system localized the direct PrP-binding domain on LRP between aa 161 and 179. Expression of an LRP mutant lacking the direct PrP-binding domain in wild-type and mutant HSPG-deficient Chinese hamster ovary cells by the Semliki Forest virus system demonstrates a second HSPG-dependent PrP-binding site on LRP. Considering the absence of LRP homodimerization and the direct and indirect LRP–PrP interaction sites, we propose a comprehensive model for the LRP–PrP–HSPG complex.

Hundt, Christoph; Peyrin, Jean-Michel; Haik, Stephane; Gauczynski, Sabine; Leucht, Christoph; Rieger, Roman; Riley, Maria Louise; Deslys, Jean-Philippe; Dormont, Dominique; Lasmezas, Corinne Ida; Weiss, Stefan




Microsoft Academic Search

Resumo: A preocupação com questões ambientais tem crescido nos últimos anos motivada, principalmente, pela busca da sociedade por conhecimentos que apontem amenização e soluções de problemas tanto em áreas urbanas como rurais. Diante desta preocupação e utilizando como base teórico-metodológica a Geomorfologia Ambiental, este trabalho apresenta um estudo geoambiental do curso médio da bacia hidrográfica do rio Verde, localizado no

Maristela Moresco Mezzomo; Andreia Quintão Soares


Effect of DA-9701 on Colorectal Distension-Induced Visceral Hypersensitivity in a Rat Model  

PubMed Central

Background/Aims DA-9701 is a newly developed drug made from the vegetal extracts of Pharbitidis semen and Co-rydalis tuber. The aim of this study was to evaluate the effect of DA-9701 on colorectal distension (CRD)-induced visceral hypersensitivity in a rat model. Methods Male Sprague-Dawley rats were subjected to neonatal colon irritation (CI) using CRD at 1 week after birth (CI group). At 6 weeks after birth, CRD was applied to these rats with a pressure of 20 to 90 mm Hg, and changes in the mean arterial pressure (MAP) were measured at baseline (i.e., without any drug administration) and after the administration of different doses of DA-9701. Results In the absence of DA-9701, the MAP changes after CRD were significantly higher in the CI group than in the control group at all applied pressures. In the control group, MAP changes after CRD were not significantly affected by the administration of DA-9701. In the CI group, however, the administration of DA-9701 resulted in a significant decrease in MAP changes after CRD. The administration of DA-9701 at a dose of 1.0 mg/kg produced a more significant decrease in MAP changes than the 0.3 mg/kg dose. Conclusions The administration of DA-9701 resulted in a significant increase in pain threshold in rats with CRD-induced visceral hypersensitivity.

Kim, Eun Ran; Min, Byung-Hoon; Lee, Tae Ho; Son, Miwon; Rhee, Poong-Lyul




Microsoft Academic Search

O complexo carnes é um dos principais componentes da pauta de exportação brasileira. O objetivo do artigo é analisar a evolução e a competitividade das exportações do complexo carnes brasileiro e os sub-complexos após 1990. Os procedimentos metodológicos envolvem a realização de uma revisão da literatura sobre competitividade e comércio internacional e coleta e tratamento de dados do complexo carnes

Luiz Gustavo Antonio De Souza; Vanderlei Jose Sereia; Marcia Regina Gabardo Camara; Marcia Goncalves Pizaia



O Papel da Certificação do Profissional de TI na Qualidade do Software: uma Investigação Preliminar  

Microsoft Academic Search

Resumo Desenvolver software com qualidade é muitas vezes considerado ilusório, dada a dificuldade de mensuração neste sentido. Além desta abordagem tradicional de determinar se o software é bom ou não, têm surgido outras abordagens alternativas para a determinação da qualidade do software, destacando-se hoje a determinação da maturidade do processo e o reconhecimento, via certificação, dos profissionais de desenvolvimento de

Alessandro Nunes de Souza; Edimara Mezzomo Luciano


Forma e funzioni delle reti di imprese: evidenze da una ricerca nel settore vitivinicolo  

Microsoft Academic Search

Il tema delle reti di imprese, già da tempo studiato, si presenta ora in tutta la sua rilevanza. La rete, infatti, costituisce uno strumento per incrementare la competitività delle imprese e per decretare il successo delle strategie da queste adottate. Il recente intervento che ha introdotto il contratto di rete testimonia anche il vivo interesse del legislatore. L’attuazione dello strumento

Federica Casarosa; Marco Gobbato



Tuning PEG-DA hydrogel properties via solvent-induced phase separation (SIPS)†  

PubMed Central

Poly(ethylene glycol) diacrylate (PEG-DA) hydrogels are widely utilized to probe cell-material interactions and ultimately for a material-guided approach to tissue regeneration. In this study, PEG-DA hydrogels were fabricated via solvent-induced phase separation (SIPS) to obtain hydrogels with a broader range of tunable physical properties including morphology (e.g. porosity), swelling and modulus (G?). In contrast to conventional PEG-DA hydrogels prepared from an aqueous precursor solution, the reported SIPS protocol utilized a dichloromethane (DCM) precursor solution which was sequentially photopolymerized, dried and hydrated. Physical properties were further tailored by varying the PEG-DA wt% concentration (5 wt%–25 wt%) and Mn (3.4k and 6k g mol ?1). SIPS produced PEG-DA hydrogels with a macroporous morphology as well as increased G? values versus the corresponding conventional PEG-DA hydrogels. Notably, since the total swelling was not significantly changed versus the corresponding conventional PEG-DA hydrogels, pairs or series of hydrogels represent scaffolds in which morphology and hydration or G? and hydration are uncoupled. In addition, PEG-DA hydrogels prepared via SIPS exhibited enhanced degradation rates.

Bailey, Brennan Margaret; Hui, Vivian; Fei, Ruochong



76 FR 19903 - Special Conditions: Diamond Aircraft Industry Model DA-40NG; Diesel Cycle Engine  

Federal Register 2010, 2011, 2012, 2013

...23-250-SC Special Conditions: Diamond Aircraft Industry Model DA-40NG; Diesel...conditions are issued for the Diamond Aircraft Industry (DAI) GmbH model DA-40NG the Austro Engine GmbH model E4 aircraft diesel engine (ADE) using...



Influência da Proximidade Geográfica na Dinâmica Inovativa de Firmas Localizadas em Sistemas Locais de Inovação  

Microsoft Academic Search

Propõe-se uma discussão a respeito da compreensão da dinâmica inovativa de firmas pertencentes a Sistemas Locais de Produção (SLPs). O objetivo é compreender a dinâmica inovativa de firmas localizadas em SLPs por meio das características dos fluxos de informação e conhecimento estabelecidos pelas firmas. Para tanto, foi realizada uma investigação de campo comparativa entre dois aglomerados do segmento produtor de

Janaína Ruffoni; Wilson Suzigan



Pa?u Ayurv?da (veterinary medicine) in Garudapur??a.  


The history of veterinary medicine is closely tied to the development of human medicine. Evidence of animal medicine has been found in ancient civilizations, such as those of the Hindu, Babylonians, Hebrews, Arabs, Greeks, and Romans. Ancient Indian literature in the form of the holy V?da, Pur?na, Br?hma?a, epics, etc. is flooded with information on animal care. The Pur??a are ancient scriptures discuss varied topics like devotion to God and his various aspects, traditional sciences like Ayurv?da, Jy?ti?a (Astrology), cosmology, concepts like dharma, karma, reincarnation and many others. The treatment of animal diseases using Ayurvedic medicine has been mentioned in Garudapur?na, Agnipur??a, Atri-samhit?, Matsyapur??a and many other texts. The Garudapur??a is one of the important S?ttvika pur?na, the subject matter is divided into two parts, viz. P?rvakha?da (first part) and an Uttarakha?da (subsequent part). Gav?yurv?da, Gaj?yurv?da narrated briefly and A?v?yurv?da described detailly in P?rvakha?da. PMID:19580108

Varanasi, Subhose; Narayana, A



Os direitos da mulher à terra e os movimentos sociais rurais na reforma agrária brasileira  

Microsoft Academic Search

R R R R Resumo: esumo: esumo: esumo: esumo: Este artigo examina a evolução da reivindicação dos direitos da mulher à terra na reforma agrária brasileira sob o prisma dos três principais movimentos sociais rurais: o Movimento dos Trabalhadores Rurais Sem Terra (MST), os sindicatos rurais e o movimento autônomo de mulheres rurais. O mérito maior por levantar a questão

Carmen Diana Deere



Associação movimento solidário colméia : um espaço para o resgate da cidadania das mulheres  

Microsoft Academic Search

Este trabalho tem como objetivo apresentar dados de uma investigação que o Grupo de Pesquisa Sexualidade e Escola (GESE) da Fundação Universidade Federal do Rio Grande (FURG) vem realizando junto às integrantes da Associação Movimento Solidário Colméia. Foram realizados vários encontros que funcionaram como um espaço narrativo em que as mulheres participaram ouvindo, contando e contrapondo histórias e fatos de

Guiomar Freitas Soares



Eunotiaceae (Bacillariophyceae) em igarapés da Amazônia Central, Manaus e Presidente Figueiredo, Brasil  

Microsoft Academic Search

RESUMO Estudos florísticos e taxonômicos envolvendo diatomáceas são escassos para a região amazônica. As publicações existentes incluem registros de diatomáceas da Amazônia brasileira, do Equador, da Colômbia e do Peru e comumente mostram que Eunotia e Actinella (Eunotiaceae) são gêneros bem representados nessa região. A maioria dos igarapés amazônicos costuma apresentar potencial hidrogeniônico (pH) ácido, característica aquática que promove o

Fernanda Ferrari; Letícia Knechtel Procopiak; Yamile Benaion Alencar; Thelma Alvim Veiga Ludwig




Microsoft Academic Search

Tendo em vista os recentes avanços da ciência, sobretudo na área de manipulação genética, a questão da clonagem humana surge como um dos grandes desafios a ser encarado. Nesse trabalho buscamos fazer uma análise não só jurídica, mas também técnica, ética e moral do tema, tendo como ponto de partida um estudo sobre a Bioética, passando pelo surgimento do Biodireito

Bernardo Abreu de Medeiros


A emergência da categoria “agricultor familiar” como sujeito de direitos na trajetória do sindicalismo rural brasileiro  

Microsoft Academic Search

O processo de reconhecimento dos agricultores familiares como sujeitos de direitos apesar de ser recente quando pensado a partir da trajetória do sindicalismo rural brasileiro demonstra ter suas primeiras raízes ainda na constituição da legislação trabalhista-sindical dos anos de 1930. Visando explorar esse processo o artigo tem por objetivo analisar a emergência dos agricultores familiares como sujeitos de direitos na

Everton Lazzaretti Picolotto



Impacto da redução dos encargos trabalhistas sobre a formalização e o salário das empregadas domésticas  

Microsoft Academic Search

O presente artigo faz uma avaliação da polarização de renda no Brasil para os anos de 1981 e 2003. Após diferenciar os conceitos de polarização e de desigualdade de renda, calculamos o índice de polarização da renda no Brasil, com base na metodologia proposta por Esteban, Gradin e Ray (1999). Os resultados indicam que a bipolarização se manteve praticamente constante

Maria Isabel Accoroni Theodoro; Luiz Guilherme Scorzafave



Compatibilizando Direitos Sociais com Competividade: Fiscais do Trabalho e a Implementação da Legislação Trabalhista no Brasil  

Microsoft Academic Search

Este estudo trata da seguinte questão: como compatibilizar direitos trabalhistas e proteção social com competitividade e produtividade das empresas? Na América Latina, há um intenso debate sobre a perversidade da legislação trabalhista (considerada prejudicial para os trabalhadores e para as empresas) e, em resposta, a política recomendada atualmente enfatiza a desregulamentação e a flexibilização dos direitos trabalhistas existentes. Em contraposição

Roberto Pires



DaRT: A Java Tool for Retrieving Archived Telescope Data  

NASA Astrophysics Data System (ADS)

DaRT is a client-side Java graphical user interface used for simultaneous retrieval of multiple documents and/or archived scientific instrument data sets which can be accessed via the World Wide Web. DaRT is currently used worldwide by astronomers to retrieve data sets from the BIMA Data Archive. DaRT can be configured to support data set retrieval from multiple data archives; an archivist can configure DaRT to support her archive simply by creating a short text file. Optionally, DaRT can support more features of an archive if more properties are added to this configuration file and/or by supplying an archive-specific Java class.

Mehringer, D. M.; Plante, R. L.


Changes in the pathogenicity of relapsed diminazene aceturate (DA)-resistant Trypanosoma brucei brucei as the trypanosomes are transmitted from DA-treated hosts to another set of animals  

Microsoft Academic Search

This study investigated the changes in pathogenicity of relapsed diminazene aceturate (DA)-resistant Trypanosoma brucei brucei (TBB) as the trypanosomes are transmitted from DA-treated hosts to another set of animals. The Federe strain of T. brucei brucei which was known to be DA resistant was used to infect two groups of rats, one of which was treated at the peak of

John Ikechukwu Ihedioha; Chinelo J. Onuma; Christian O. Okorie-Kanu; Thelma E. Ihedioha



Asteroseismology of pulsating DA white dwarfs with fully evolutionary models  

NASA Astrophysics Data System (ADS)

We present a new approach for asteroseismology of DA white dwarfs that consists in the employment of a large set of non-static, physically sound, fully evolutionary models representative of these stars. We already have applied this approach with success to pulsating PG1159 stars (GW Vir variables). Our white dwarf models, which cover a wide range of stellar masses, effective temperatures, and envelope thicknesses, are the result of fully evolutionary computations that take into account the complete history of the progenitor stars from the ZAMS. In particular, the models are characterized by self-consistent chemical structures from the centre to the surface, a crucial aspect of white dwarf asteroseismology. We apply this approach to an ensemble of 44 bright DAV (ZZ Ceti) stars.

Romero, A. D.; Córsico, A. H.; Althaus, L. G.; Miller Bertolami, M. M.



[Regarding the Manuscript D " Dell' occhio " of Leonardo da Vinci].  


Leonardo da Vinci's Manuscript D consists of five double pages sheets, which, folded in two, comprise ten folios. This document, in the old Tuscan dialect and mirror writing, reveals the ideas of Leonardo on the anatomy of the eye in relation to the formation of images and visual perception. Leonardo explains in particular the behavior of the rays in the eye in terms of refraction and reflection, and is very mechanistic in his conception of the eye and of the visual process. The most significant innovations found in these folios are the concept of the eye as a camera obscura and the intersection of light rays in the interior of the eye. His texts nevertheless show hesitation, doubts and a troubled confusion, reflecting the ideas and uncertainties of his era. He did not share his results in his lifetime, despite both printing and etching being readily available to him. PMID:19852385

Heitz, Robert F




SciTech Connect

We present a spectroscopically identified catalog of 70 DA white dwarfs (WDs) from the LAMOST pilot survey. Thirty-five are found to be new identifications after cross-correlation with the Eisenstein et al. and Villanova catalogs. The effective temperature and gravity of these WDs are estimated by Balmer lines fitting. Most of them are hot WDs. The cooling times and masses of these WDs are estimated by interpolation in theoretical evolution tracks. The peak of the mass distribution is found to be {approx}0.6 M {sub Sun }, which is consistent with prior work in the literature. The distances of these WDs are estimated using the method of synthetic spectral distances. All of these WDs are found to be in the Galactic disk from our analysis of space motions. Our sample supports the expectation that WDs with high mass are concentrated near the plane of the Galactic disk.

Zhao, J. K.; Luo, A. L.; Zhao, G. [Key Laboratory of Optical Astronomy, National Astronomical Observatories, Chinese Academy of Sciences, Beijing 100012 (China); Oswalt, T. D., E-mail:, E-mail:, E-mail:, E-mail: [Physics and Space Science Department, Florida Institute of Technology, Melbourne, FL 32901 (United States)



Discovery of Photospheric Germanium in Hot DA White Dwarfs  

NASA Astrophysics Data System (ADS)

We report the identification of Ge IV resonance lines in ultraviolet spectra of the hot DA white dwarfs Feige 24, G191-B2B, and GD 246. The lines originate in the stellar photosphere, and we measure low Ge/H abundance ratios ranging between -8.0 and -8.7. We also tentatively identify a resonance line of Sn IV blended with an Fe V line in the spectrum of G191-B2B. The presence of germanium extends our knowledge of the abundance pattern in hot white dwarfs beyond the iron group. The abundance ratio appears nearly solar, which implies either that the germanium abundance mixture in these stars has remained unaltered since leaving the main sequence or that diffusion processes (e.g., selective radiation pressure) are coincidentally reproducing a solar Ge/H ratio.

Vennes, Stéphane; Chayer, Pierre; Dupuis, Jean



Heavy-element abundance patterns in hot DA white dwarfs  

NASA Astrophysics Data System (ADS)

We present a series of systematic abundance measurements for 25 hot DA white dwarfs in the temperature range ~20 000-110 000 K, based on far-ultraviolet spectroscopy with the Space Telescope Imaging Spectrograph (STIS)/Goddard High Resolution Spectrograph (GHRS) on-board Hubble Space Telescope, IUE and FUSE. Using our latest heavy-element blanketed non-local thermodynamic equilibrium (non-LTE) stellar atmosphere calculations we have addressed the heavy-element abundance patterns, making completely objective measurements of abundance values and upper limits using a ?2 fitting technique to determine the uncertainties in the abundance measurements, which can be related to the formal upper limits in those stars where particular elements are not detected. We find that the presence or absence of heavy elements in the hot DA white dwarfs largely reflects what would be expected if radiative levitation is the supporting mechanism, although the measured abundances do not match the predicted values very well, as reported by other authors in the past. Almost all stars hotter than ~50 000 K contain heavy elements. For most of these the spread in element abundances is quite narrow and similar to the abundances measured in G191-B2B. However, there is an unexplained dichotomy at lower temperatures with some stars having apparently pure H envelopes and others having detectable quantities of heavy elements. The heavy elements present in these cooler stars are often stratified, lying in the outermost layers of the envelope. A few strong temperature/evolutionary effects are seen in the abundance measurements. There is a decreasing Si abundance with temperature, the N abundance pattern splits into two groups at lower temperature and there is a sharp decline in Fe and Ni abundance to zero, below ~50 000 K. When detected, the Fe and Ni abundances maintain an approximately constant ratio, close to the cosmic value of ~20. For the hottest white dwarfs observed by STIS, the strongest determinant of abundance appears to be gravity.

Barstow, M. A.; Good, S. A.; Holberg, J. B.; Hubeny, I.; Bannister, N. P.; Bruhweiler, F. C.; Burleigh, M. R.; Napiwotzki, R.



A estabilidade dos PAHS em função da energia da radiação interestelar nas faixas UV e raios-X  

NASA Astrophysics Data System (ADS)

A nebulosa CRL 618, uma proto-nebulosa planetária cuja nuvem molecular espessa envolve uma estrela B0, contém uma grande quantidade de C2H2 e CH4. Estas moléculas são consideradas os tijolos da criação de grandes moléculas carbonadas como os Hidrocarbonetos Policíclicos Aromáticos (PAHs). Esta nebulosa, por estar exposta a intensos campos de UV e Raios-X, é uma região de fotodissociação molecular que propicia a formação de novas moléculas, confirmada pela presença de C4H2 e C6H6 (Benzeno), que é a unidade básica dos PAHs. Atribui-se a esta família de moléculas orgânicas duas propriedades fundamentais, a resistência para sobreviver ao campo de radiação UV interestelar e a geração das bandas de emissão não identificadas (UIR) observadas no infravermelho. No entanto, alguns autores questionam a resistência dos PAHs ao campo de radiação UV interestelar. Empregando a técnica de Espectrometria de Massas por Tempo de Vôo, no modo de coincidência fotoelétron-fotoíon, estudamos a ionização e fragmentação das seguintes moléculas: Benzeno, Benzeno deuterado, Naftaleno, Antraceno e Fenantreno. Utilizamos uma fonte de Hélio monocromática em 21,21 eV (584,5 Å) e a radiação Síncroton do Laboratório Nacional de Luz Síncroton (LNLS) em diferentes energias nas proximidades da borda do C 1s ( 290 eV). Comprovamos a estabilidade dos PAHs sob ação de UV (21,21 eV), onde eles apresentam um baixo nível de fotodissociação, produzindo fragmentos ionizados com rendimento total na ordem de 5 por cento em relação ao íon molecular pai. Entretanto, em altas energias, na faixa de Raios-X, a quebra destas moléculas torna-se mais intensa, com a produção de muitos fragmentos. Como uma das rotas de fragmentação do Naftaleno é [(C10H8) = > (C6H6+) + (C4H2) + (e-)], e como temos as evidências observacionais da existência do C4H2 e C6H6 na nebulosa CRL 618, sugerimos que este ambiente também possui o Naftaleno.

Pinotti, R.; Costa, R. K.; Boechat-Roberty, H. M.; Lago, A.; Souza, G. B.



Avanços no entendimento da base genética de doenças hered- itárias monogênicas da barreira epidérmica: novas pistas para os principais genes que podem estar envolvidos na patogênese da dermatite atópica.* Advances in understanding the genetic basis of inherited single gene skin barrier disorders: new clues to key genes that may be involved in the pathogenesis of atopic dermatitis  

Microsoft Academic Search

2 Resumo: O maior conhecimento sobre as de sequências genômicas de DNA e as bases de dados gené- ticas levou à caracterização da base molecular de várias doenças hereditárias de pele. Nesta revisão resumimos algumas das descobertas recentes mais importantes quanto à definição das mutações patogênicas que causam as doenças hereditárias da barreira cutânea, levando a descamação ou aumento da

Joey E Lai-Cheong; John A. McGrath


Endogenous dopamine (DA) modulates (3H)spiperone binding in vivo in rat brain  

SciTech Connect

(3H)spiperone (SPI) binding in vivo, biochemical parameters and behavior were measured after modulating DA levels by various drug treatments. DA releasers and uptake inhibitors increased SPI binding in rat striatum. In other brain areas, the effects were variable, but only the pituitary remained unaffected. Surprisingly, nomifensine decreased SPI binding in frontal cortex. The effects of these drugs were monitored by measuring DA, serotonin (5-HT) and their metabolites in the same rats. The increased SPI binding in striatum was parallel to the locomotor stimulation with the following rank order: amfonelic acid greater than nomifensine greater than D-amphetamine greater than or equal to methylphenidate greater than amineptine greater than bupropion. Decreasing DA levels with reserpine or alpha-methyl-para-tyrosine reduced SPI binding by 45% in striatum only when both drugs were combined. In contrast, reserpine enhanced SPI binding in pituitary. Thus, the amount of releasable DA seems to modulate SPI binding characteristics. It is suggested that in vivo, DA receptors are submitted to dynamic regulation in response to changes in intrasynaptic concentrations of DA.

Bischoff, S.; Krauss, J.; Grunenwald, C.; Gunst, F.; Heinrich, M.; Schaub, M.; Stoecklin, K.V.; Vassout, A.; Waldmeier, P.; Maitre, L. (Research Department, CIBA-GEIGY Ltd., Basel (Switzerland))



Characterization of a 95 kDa high affinity human high density lipoprotein-binding protein.  


A new human 95 kDa high density lipoprotein (HDL)-binding protein (HBP) corresponding to a high affinity HDL-binding site with K(d) = 1.67 microg/mL and a capacity of 13.4 ng/mg was identified in human fetal hepatocytes. The HDL binding with the 95 kDa HBP plateaus at 2.5-5 microg/mL under reducing and nonreducing conditions. The association of HDL(3) with the 95 kDa HBP plateaued in 15-30 min while dissociation was complete in 30 min. HDL(3), apoA-I, and apoA-II were recognized by the 95 kDa HBP while low density lipoproteins (LDL) and tetranitromethane-modified HDL were not. The 95 kDa HBP predominantly resides on the surface of cells since trypsin treatment of HepG2 cells eliminated nearly 70% of HDL binding. All studied human cells and cell lines (HepG2, Caco-2, HeLa, fibroblasts, SKOV-3, PA-I) demonstrated the presence of the 95 kDa protein. Both RT-PCR and Western blotting for HB-2/ALCAM were negative in human fetal hepatocytes while Gp96/GRP94 was clearly differentiated from the 95 kDa HBP by two-dimensional electrophoretic mobility. Moreover, deglycosylation of HepG2 membrane preparations did not affect either HDL binding to the 95 kDa HBP or its size, while in contrast it affected the molecular weights of HB-2/ALCAM and SR-BI/CLA-1. We conclude that the 95 kDa HBP is a new HDL receptor candidate widely expressed in human cells and cell lines. PMID:11284697

Bocharov, A V; Vishnyakova, T G; Baranova, I N; Patterson, A P; Eggerman, T L



High resolution structure of cleaved Serpin 42 Da from Drosophila melanogaster  

PubMed Central

Background The Drosophila melanogaster Serpin 42 Da gene (previously Serpin 4) encodes a serine protease inhibitor that is capable of remarkable functional diversity through the alternative splicing of four different reactive centre loop exons. Eight protein isoforms of Serpin 42 Da have been identified to date, targeting the protease inhibitor to both different proteases and cellular locations. Biochemical and genetic studies suggest that Serpin 42 Da inhibits target proteases through the classical serpin ‘suicide’ inhibition mechanism, however the crystal structure of a representative Serpin 42 Da isoform remains to be determined. Results We report two high-resolution crystal structures of Serpin 42 Da representing the A/B isoforms in the cleaved conformation, belonging to two different space-groups and diffracting to 1.7 Å and 1.8 Å. Structural analysis reveals the archetypal serpin fold, with the major elements of secondary structure displaying significant homology to the vertebrate serpin, neuroserpin. Key residues known to have central roles in the serpin inhibitory mechanism are conserved in both the hinge and shutter regions of Serpin 42 Da. Furthermore, these structures identify important conserved interactions that appear to be of crucial importance in allowing the Serpin 42 Da fold to act as a versatile template for multiple reactive centre loops that have different sequences and protease specificities. Conclusions In combination with previous biochemical and genetic studies, these structures confirm for the first time that the Serpin 42 Da isoforms are typical inhibitory serpin family members with the conserved serpin fold and inhibitory mechanism. Additionally, these data reveal the remarkable structural plasticity of serpins, whereby the basic fold is harnessed as a template for inhibition of a large spectrum of proteases by reactive centre loop exon ‘switching’. This is the first structure of a Drosophila serpin reported to date, and will provide a platform for future mutational studies in Drosophila to ascertain the functional role of each of the Serpin 42 Da isoforms.



Application of DA-Preconditioned FINN for Electric Power System Fault Detection  

NASA Astrophysics Data System (ADS)

This paper proposes a hybrid method of Deterministic Annealing (DA) and Fuzzy Inference Neural Network (FINN) for electric power system fault detection. It extracts features of input data with two-staged precondition of Fast Fourier Transform (FFT) and DA. FFT is useful for extracting the features of fault currents while DA plays a key role to classify input data into clusters in a sense of global classification. FINN is a more accurate estimation model than the conventional artificial neural networks (ANNs). The proposed method is successfully applied to data obtained by the Tokyo Electric Power Company (TEPCO) power simulator.

Itagaki, Tadahiro; Mori, Hiroyuki; Yamada, Takeshi; Urano, Shoichi


2012 DA14 and Chelyabinsk: Same Day Coincidence?  

NASA Astrophysics Data System (ADS)

A long anticipated near-miss by near-Earth asteroid (NEA) 2012 DA14 on 15 February 2013 was upstaged 16 hours earlier by the impact and atmospheric explosion of a ~20 m NEA over Chelyabinsk, Russia. DA14 was earlier estimated to be 45 m across and passage at a distance under geosynchronous satellites was considered to be a record close approach by an object of that size, a once-in-40-years event. Actual Earth impact by a 20 m NEA had been estimated to be a once-in-200-years event. A simplistic calculation gives a probability of these happening on the same day of 1-in-a-billion. Within hours of the Chelyabinsk impact, it was recognized and widely reported that the two asteroids were in very different orbits and could not, at least in any readily understandable way, be physically related to each other (e.g. fragments of the same precursor body). Also, some early reports suggested that the two asteroids had very different compositions, further undermining the possibility they were pieces of the same body. (It seems unlikely, though certainly conceivable, that the two NEAs could have different compositions yet have some kind of causal connection resulting in the same-day events.) Nevertheless, incredibly tiny probabilities beg for an explanation. Here, with the benefit of hindsight, I re-examine issues that affect the probabilities, such as size, albedo, probable composition, and numbers of NEAs of these sizes. I also consider difficult issues of framing the apparent coincidence, which dominate other uncertainties. Updated information about the physical nature of the two asteroids somewhat modifies the original estimates of probabilities. Moreover, more proper ways of framing elements of the coincidence considerably reduce the improbability of the same-day events, but not nearly to the level (e.g. 1-in-1000 or perhaps 1-in-10,000) where we could rationalize dismissing the events as “just a coincidence.” The events of 15 February 2013 deserve more sophisticated investigation.

Chapman, Clark R.



Near-UV Absorption in Very Cool DA White Dwarfs  

NASA Astrophysics Data System (ADS)

The atmospheres of very cool, hydrogen-rich white dwarfs (WDs) (T eff < 6000 K) are challenging to model because of the increased complexity of the equation of state, chemical equilibrium, and opacity sources in a low-temperature, weakly ionized dense gas. In particular, many models that assume relatively simple models for the broadening of atomic levels and mostly ideal gas physics overestimate the flux in the blue part of their spectra. A solution to this problem that has met with some success is that additional opacity at short wavelengths comes for the extreme broadening of the Lyman ? line of atomic H by collisions primarily with H2. For the purpose of validating this model more rigorously, we acquired Hubble Space Telescope STIS spectra of eight very cool WDs (five DA and three DC stars). Combined with their known parallaxes, BVRIJHK, and Spitzer IRAC photometry, we analyze their entire spectral energy distribution (from 0.24 to 9.3 ?m) with a large grid of model atmospheres and synthetic spectra. We find that the red wing of the Lyman ? line reproduces the rapidly decreasing near-UV flux of these very cool stars very well. We determine better constrained values of T eff and gravity as well as upper limits to the helium abundance in their atmospheres.

Saumon, D.; Holberg, J. B.; Kowalski, P. M.



Audio annotation watermarking with robustness against DA/AD conversion  

NASA Astrophysics Data System (ADS)

In the paper we present a watermarking scheme developed to meet the specific requirements of audio annotation watermarking robust against DA/AD conversion (watermark detection after playback by loudspeaker and recording with a microphone). Additionally the described approach tries to achieve a comparably low detection complexity, so it could be embedded in the near future in low-end devices (e.g. mobile phones or other portable devices). We assume in the field of annotation watermarking that there is no specific motivation for attackers to the developed scheme. The basic idea for the watermark generation and embedding scheme is to combine traditional frequency domain spread spectrum watermarking with psychoacoustic modeling to guarantee transparency and alphabet substitution to improve the robustness. The synchronization and extraction scheme is designed to be much less computational complex than the embedder. The performance of the scheme is evaluated in the aspects of transparency, robustness, complexity and capacity. The tests reveals that 44% out of 375 tested audio files pass the simulation test for robustness, while the most appropriate category shows even 100% robustness. Additionally the introduced prototype shows an averge transparency of -1.69 in SDG, while at the same time having a capacity satisfactory to the chosen application scenario.

Qian, Kun; Kraetzer, Christian; Biermann, Michael; Dittmann, Jana




SciTech Connect

A total of {approx}640, 000 objects from the LAMOST pilot survey have been publicly released. In this work, we present a catalog of DA white dwarfs (DAWDs) from the entire pilot survey. We outline a new algorithm for the selection of white dwarfs (WDs) by fitting Sersic profiles to the Balmer H{beta}, H{gamma}, and H{delta} lines of the spectra, and calculating the equivalent width of the Ca II K line. Two thousand nine hundred sixty-four candidates are selected by constraining the fitting parameters and the equivalent width of the Ca II K line. All the spectra of candidates are visually inspected. We identify 230 DAWDs (59 of which are already included in the Villanova and SDSS WD catalogs), 20 of which are DAWDs with non-degenerate companions. In addition, 128 candidates are classified as DAWDs/subdwarfs, which means the classifications are ambiguous. The result is consistent with the expected DAWD number estimated based on the LEGUE target selection algorithm.

Zhang Yueyang; Deng Licai; Liu Chao; Carrell, Kenneth; Yang Fan; Gao Shuang; Xu Yan; Li Jing; Zhang Haotong; Zhao Yongheng; Luo Ali; Bai Zhongrui; Yuan Hailong [Key Lab for Optical Astronomy, National Astronomical Observatories, Chinese Academy of Sciences, Beijing 100012 (China); Lepine, Sebastien [Department of Astrophysics, Division of Physical Sciences, American Museum of Natural History, Central Park West at 79th Street, New York, NY (United States); Newberg, Heidi Jo; Carlin, Jeffrey L. [Department of Physics, Applied Physics and Astronomy, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY 12180 (United States); Jin Ge [University of Science and Technology of China, Hefei 230026 (China)



First results from the FINUDA experiment at DA?NE  

NASA Astrophysics Data System (ADS)

In spring 2003 the FINUDA detector was installed at the ? factory DA?NE in the Laboratori Nazionali di Frascati of INFN (Italy). In October 2003 the commissioning of the apparatus was accomplished and the first data taking started with a set of nuclear targets 6Li, 7Li, natC, 27Al, 51V. The data collection will continue until a total integrated luminosity of 250 pb is recorded. Light and medium A hypernuclei will be abundantly produced by the strangeness exchange reaction induced by the stopped K coming from the decay of ?(1020) mesons. The aim of the experiment is to simultaneously measure the excitation energy spectra of the produced hypernuclei, with a resolution better than 1 MeV, the lifetime of the ? embedded in the different hypernuclei and the partial widths ?, ? and ? for mesonic and non-mesonic hypernuclear decays. Information on neutron-rich hypernuclei and rare hypernuclear two-body decays might be available too, with the statistics that will be collected at the end of the run. In the present paper, first results concerning in-beam detector calibration, spectrometer performances and very preliminary hypernuclear formation and decay spectra will be presented.

Agnello, M.; Beer, G.; Benussi, L.; Bertani, M.; Bianco, S.; Botta, E.; Bressani, T.; Busso, L.; Calvo, D.; Camerini, P.; Cerello, P.; Dalena, B.; De Mori, F.; D'Erasmo, G.; Di Santo, D.; Fabbri, F. L.; Faso, D.; Feliciello, A.; Filippi, A.; Filippini, V.; Fiore, E. M.; Fujioka, H.; Gianotti, P.; Grion, N.; Krasnoperov, A.; Lucherini, V.; Marcello, S.; Maruta, T.; Mirfakhrai, N.; Morra, O.; Olin, A.; Pace, E.; Palomba, M.; Pantaleo, A.; Panzarasa, A.; Paticchio, V.; Piano, S.; Pompili, F.; Rui, R.; Simonetti, G.; So, H.; Tomassini, S.; Wheadon, R.; Zenoni, A.



Immune response of electroeluted detergent soluble 29 kDa antigen from Setaria digitata (Von Linstow).  


Filariasis is one of the typical parasitic infections which cause immune suppression during the course of infection in both humans and experimental animals. A 29 kDa protein isolated from detergent soluble antigen of S. digitata showed maximum inhibition of cell mediated immune response. The heat inactivated 29 kDa protein was found to be devoid of property of suppression of immune response in the host. Histological study of spleen of BALB/C mice immunized with 29 kDa protein showed changes in regions of spleen such as follicle, trabeculae, capsule, reticuloendothelial cells and eosinophils. The 29 kDa protein, the most reactive of the detergent soluble proteins produced partial suppression of immune response, thereby contributing to the factors responsible for the survival of filarial parasites in hosts. PMID:9854424

John, L; Raj, R K



ARD1, a 64-kDa bifunctional protein containing an 18-kDa GTP-binding ADP-ribosylation factor domain and a 46-kDa GTPase-activating domain.  

PubMed Central

The alpha subunits of the heterotrimeric guanine nucleotide-binding proteins (G proteins) hydrolyze GTP at a rate significantly higher than do most members of the Ras family of approximatelly 20-kDa GTP-binding proteins, which depend on a GTPase-activating protein (GAP) for acceleration of GTP hydrolysis. It has been demonstrated that an inserted domain in the G-protein alpha subunit, not present in the much smaller Ras-like proteins, is responsible for this difference [Markby, D. W., Onrust, R. & Bourne, H. R. (1993) Science 262, 1895-1900]. We report here that ARD1, a 64-kDa protein with an 18-kDa carboxyl-terminal ADP-ribosylation factor (ARF) domain, exhibited significant GTPase activity, whereas the ARF domain, expressed as a recombinant protein in Escherichia coli, did not. Addition of the 46-kDa amino-terminal extension (similarly synthesized in E. coli) to the GTP-binding ARF-domain of ARD1 enhanced GTPase activity and inhibited GDP dissociation. The kinetic properties of mixtures of the ARF and non-ARF domains were similar to those of an intact recombinant ARD1. Physical association of the two proteins was demonstrated directly by gel filtration and by using the immobilized non-ARF domain. Thus, like the alpha subunits of heterotrimeric G proteins, ARD1 appears to consist of two domains that interact to regulate the biological activity of the protein. Images Fig. 1 Fig. 3 Fig. 4

Vitale, N; Moss, J; Vaughan, M



Biogeochemical Indicators of Paleoenvironmental Changes in a Humid Region of Amazonia (Lagoa da Pata, Sao Gabriel da Cachoeira)  

NASA Astrophysics Data System (ADS)

Analysis of four sediment core collected in Lagoa da Pata lake show significant changes in the environmental history of Amazonia during the last 40,000 years. The Lagoa da Pata Lake (0°17'S, 66°40'W) is located on the top of the Morro dos Seis Lagos, an isolated hill in the extensive forested plane surface of high Rio Negro basin. The climate in the region presents mean annual precipitation around 3000 mm without dry season. Three distinct sections are clearly identified in the LPT III, LPT IV and LPT V cores. They consist of upper and lower organic-rich layers, separated by a clastic layer which represents a short period of rapid accumulation around 18 ka BP. Analysis of the organic matter composition by d13C and d15N, total Carbon and Nitrogen, sedimentary chlorophyll concentration and organic matter petrography show three different phases related to organic matter deposition. Between, at least 46,000 and 31,000 14C yr B.P., the lake presented a high water level attested by high accumulation rates of organic carbon. Values of d13C (around -32%) associated to high C/N ratio demonstrate a lignocellulosic material contribution in this phase; the second phase between the 31000 yr 14C B.P. and 18000 14C B.P. is characterised by a lower productivity attested by low sedimentary chlorophyll and total organic carbon values. The d13C values increase with medium values around -28% that may correspond to an input of organic matter rich in C4 plants (grasses). The sedimentation rate decreases significantly. High charcoal fluxes were observed in this period. At around 18000 14C yr BP, there appears to have been a sudden input of clastic material. This is represented by a sandy facies, which exhibits lower carbon contents. From 18000 14C yr B.P. to the present time occurred an increase in lacustrine productivity marked by an increase in carbon and chlorophyll derivate accumulation rates. The C/N values dropped indicting an algae organic matter contribution. It probably corresponds to a lake level rise increasing the production of the system. Increases in charcoal deposition were observed during the upper and medium Holocene. This project is part of the PROFIX/CNPq (processo 54086/01-5) and CNPq (Brazil)-IRD(France) project "PALEOTROPICA" .

Cordeiro, R. C.; Turcq, B.; Mendes, L. G.; Azeredo, J. B.; Moura, M. S.; de Oliveira, A. R.; Monteiro, F.; Santelli, R. E.



Composição florística da vegetação arbórea de um trecho de floresta estacional semidecídua em Viçosa, Minas Gerais, e espécies de maior ocorrência na região  

Microsoft Academic Search

RESUMO - É grande a demanda por estudos ecológicos em florestas que possam embasar trabalhos de recuperação, conservação da biodiversidade e apoio à legislação ambiental. Entre esses estudos, primordialmente está o levantamento da flora. Nesse sentido, este trabalho teve como objetivo determinar a composição florística de um trecho da Reserva da Biologia, em Viçosa, MG, em regeneração natural há 80

Walnir Gomes Ferreira Júnior; Alexandre Francisco da Silva; João Augusto Alves Meira Neto; Carlos Ernesto Gonçalves Reynaud Schaefer; Arildo de Souza Dias; Maíra Ignácio; Maria Cláudia Melo Pacheco de Medeiros



A Novel 90-kDa Stress Protein Induced in Fish Cells by Fish Rhabdovirus Infection  

Microsoft Academic Search

A 90 kDa cellular protein in a fish cell, CHSE-214, showed increased expression by the infection of infectious hematopoietic necrosis virus (IHNV), heat shock, 2-mercaptoethanol, copper sulfate, and cadmium sulfate, and was detected in various kinds of cells such as human, rat, and mouse cells. The molecular mass of the 90 kDa protein was different from those of the hsp90

Wha-Ja Cho; Seung-Ju Cha; Jeong-Wan Do; Jeong-Yun Choi; Joo-Yang Lee; Choon-Soo Jeong; Kyung-Jae Cho; Wan-Sung Choi; Ho-Sung Kang; Han-Do Kim; Jeong-Woo Park



Direct binding of Torpedo syntrophin to dystrophin and the 87 kDa dystrophin homologue  

Microsoft Academic Search

Syntrophin, a 58-kDa membrane-associated protein, is one component of a protein complex associated with dystrophin and other members of the dystrophin family, including the 87-kDa homologue (87K protein). To characterize interactions between syntrophin and 87K protein, we used an in vitro overlay binding assay. We demonstrate that purified Torpedo syntrophin binds directly to dystrophin and 87K. By expressing overlapping regions

Timothy M. Dwyer; Stanley C. Froehner



Determinação de regiões de órbitas diretas estáveis ao redor da Lua  

NASA Astrophysics Data System (ADS)

Neste Trabalho estamos investigando regiões do sistema Terra-Lua que contêm órbitas diretas estáveis ao redor da Lua visando a utilização destas órbitas em futuras missões de veículos espaciais como alternativas de trajetórias com baixo custo de manutenção. Adotando-se o problema restrito de três corpos, Terra-Lua-partícula, a localização e a extensão destas regiões de estabilidade foram investigadas numericamente considerando como estável toda órbita que permanece ao redor a Lua por, no mínimo, 1000 dias com energia de dois corpos da partícula em relação à Lua negativa. A estabilidade de tais regiões está associada a duas famílias de órbitas periódicas simples H1 e H2 e a órbitas quase-periódicas associadas a elas. Uma vez identificadas as regiões de estabilidade passamos a analisá-las introduzindo, paulatinamente, outras interações relevantes ao sistema como as perturbações do Sol (via problema restrito de quatro corpos), das marés, da pressão de radiação e do achatamento terrestre. Os resultados encontrados até o momento mostram que a perturbação do Sol é a única que reduz significativamente o tamanho das regiões de estabilidade. Também estamos investigando o comportamento de tais regiões quando características intrínsecas do sistema como as excentricidades das órbitas da Terra e da Lua e a inclinação da órbita Lua são consideradas. Com este estudo estamos reunindo informações que nos permitirão compreender a evolução das regiões de estabilidade no sistema Terra-Lua, bem como estabelecer parâmetros adequados para a utilização das trajetórias estáveis em futuras missões espaciais. Agradecimentos: Este projeto conta com o apoio do CNPq, da Capes e da Fapesp.

de Melo, C. F.; Winter, O. C.; Vieira Neto, E.



Metal deposits in the Da Hinggan Mountains, NE China: styles, characteristics, and exploration potential  

Microsoft Academic Search

The Da Hinggan Mountains mineral province (DHMP), northeastern China, is divided into three tectonic units and corresponding metallogenic belts. The tectonic units of the Da Hinggan Mountains are the Erguna fold zone on the northwest, the Hercynian fold zone on the north, and the Hercynian fold zone on the south. The corresponding metallogenic belts are the Erguna Cu-Pb-Zn-Ag-Mo-Au belt of

Qingdong Zeng; Jianming Liu; Changming Yu; Jie Ye; Hongtao Liu



Detecção da fase impulsiva de uma explosão solar gigante até 405 GHz  

NASA Astrophysics Data System (ADS)

A explosão ocorrida no dia 25/08/2001 foi uma das mais intensas do presente ciclo solar em ondas de rádio de altas frequências. Foram medidas em ondas milimétricas e submilimétricas, aproximadamente, 105 e vários milhares de unidades de fluxo solar, respectivamente. Apresentamos um estudo deste evento em múltiplas frequências, desde microondas (1GHz), até ondas submilimétricas (405 GHz) detectadas pelo Telescópio Solar para ondas Submilimétricas (SST). Esta base de dados foi complementada utilizando-se o experimento Yohkoh, incluindo a emissão em raios-X duros e raios-g (até 100 MeV), e imagens em raios-X moles da região ativa envolvida. Enfocamos e discutimos principalmente os seguintes aspectos da fase impulsiva do evento: (i) as implicações deduzidas do espectro eletromagnético, obtido pela primeira vez até 405 GHz; (ii) a dinâmica da região ativa. Os resultados mostram que para explicar o espectro rádio observado, são necessários entre 3.5×1037 e 1.5×1039 elétrons acelerados acima de 20 keV em uma região de campo magnético entre 300 e 800 Gauss. A estimativa do fluxo de fótons que seria produzido por estes elétrons, mostra que grande parte deles não precipitou na baixa atmosfera. A evolução temporal da emissão em raios-X moles revela que a configuração magnética da região ativa foi muito dinâmica durante a fase impulsiva da explosão. Em particular, mostramos que a produção dos elétrons altamente energéticos foi iniciada junto com a aparição, na baixa coroa solar, de um novo sistema compacto de estruturas magnéticas. Este fato sugere que os locais de aceleração estão localizados na baixa atmosfera do Sol, como resultado da interação entre o novo sistema compacto e o campo magnético ambiente da região ativa.

Raulin, J.-P.; Makhmutov, V.; Kaufmann, P.; Pacini, A. A.; Luethi, T.; Hudson, H. S.; Gary, D. E.; Yoshimori, M.



The use of Therav?da Buddhist practices and perspectives in mindfulness-based cognitive therapy  

Microsoft Academic Search

This study explores and assesses the nature and practice of Mindfulness-Based Cognitive Therapy (MBCT) from the perspective of Therav?da Buddhism. It is particularly concerned with how both models of training understand and apply ‘mindfulness’. The approach here is, firstly, to examine how the Therav?da understands and employs mindfulness and, secondly, to explore, and more accurately contextualize, the work of MBCT.

Richard Gilpin



A 50-kDa variant form of human surfactant protein D.  


The dominant form of human surfactant protein D (SP-D) is a multimeric collagenous glycoprotein composed of monomeric subunits that have a molecular mass of 43 kDa under reducing conditions. However, in evaluating monoclonal antibodies to human SP-D, an additional monomeric subunit was identified with a reduced molecular mass of 50 kDa. This 50-kDa variant was detected in approximately half of the samples evaluated and was found in lavage fluid from normal subjects, patients with alveolar proteinosis or idiopathic pulmonary fibrosis and in amniotic fluid. This 50-kDa variant had the same amino-terminal sequence, amino acid composition and apparent size of the carboxy-terminal collagenase-resistant fragment (20 kDa) as the 43-kDa subunit. The major difference was in the amino-terminal portion of the molecule and was due to altered glycosylation, as determined by carbohydrate staining, chemical deglycosylation, treatment with N-glycanase and neuraminidase and reduced signals for threonine at positions 5, 9 and 10 during amino-terminal sequencing. After gel filtration chromatography, the 50-kDa form was not present in the high molecular weight fraction, which is commonly used in purification of SP-D, but was found only in the smaller molecular weight fraction of monomers and trimers of SP-D. In conclusion, the 50 kDa-form of surfactant protein D is produced by post-translational glycosylation and does not form higher ordered oligomers, but its precise physiological function remains to be determined. PMID:9864012

Mason, R J; Nielsen, L D; Kuroki, Y; Matsuura, E; Freed, J H; Shannon, J M



Temperature and Gravity Dependence of Trace Element Abundances in Hot DA White Dwarfs (94-EUVE-094)  

NASA Technical Reports Server (NTRS)

EUV spectroscopy has shown that DA white dwarfs hotter than about 45,000 K may contain trace heavy elements, while those hotter than about 50,000 K almost always have significant abundances of trace heavy elements. One of our continuing challenges is to identify and determine the abundances of these trace constituents, and then to relate the observed abundance patterns to the present conditions and previous evolutionary histories of the hot DA white dwarfs.

Finley, David S.



Multiple vital functions of the daughterless (da) gene in Drosophila melanogaster and factors influencing its expression.  


Multiple sub-vital effects, here designated as reductions in fitness S1-S5, for the second chromosome gene 'daughterless' (da) of Drosophila melanogaster were described as (S1) a recessive maternal lethality for daughters, (S2) a reduced fertility of da/da females, (S3) a recessive sub-vital zygotic effect, (S4) a recessive female-specific zygotic effect and (S5) a recessive maternal interaction effect on sons. (For S1-3 see also: Bell, 1954; Sandler, 1972; Cline, 1976). These five distinct effects were initially quantified from estimates of viability in single generation crossing experiments. Dynamic estimates of these fitness parameters were obtained by fitting the elimination rate of da from a series of large random mating cage populations to a recurrence equation by the method of minimum chi-square. The stability of these estimates discerns those effects which are truly pleiotropic versus those due to linked genes. The dynamic estimates supported only S1 and S4 effects. Evidence for S2 and S5 was indeterminate, but the S3 effect was rejected (P less than 0.01). The observed reduction in fitness, supposedly due to this recessive zygotic effect for da, was most likely the result of linked deleterious genes. These results indicate that pleiotropic vital effects observed in single generation test-cross matings may be caused by linked genes rather than the specific mutant per se. This problem is of particular importance when the mutant allele has been maintained with a balancer chromosome. Experiments on the rescue of daughters from da/da mothers with low temperatures during embryogenesis and with dechorionation of eggs were described in which the findings failed to confirm previously reported actions of the da gene. Modifying genes rather than environmental variables were cited as the probable cause for these conflicting results. PMID:3146524

Muir, W M; Bell, A E



Immunoreactivity of a 10-kDa antigen of Mycobacterium tuberculosis.  


Identification of Ag of Mycobacterium tuberculosis recognized by T cells is essential to understanding the pathogenesis of tuberculosis and mechanism(s) of resistance to infection. Previous studies evaluating the immunoreactivity of nitrocellulose transfers of M. tuberculosis Ag separated by SDS-PAGE indicated that a high proportion of M. tuberculosis-reactive T cell lines proliferate in response to a 10-kDa Ag. We therefore purified this Ag from M. tuberculosis culture filtrates and evaluated its immunoreactivity in patients with tuberculous infection. Proliferative responses of PBMC to the 10-kDa Ag were similar to those induced by whole M. tuberculosis and greater than those elicited by other proteins isolated from culture filtrate. Furthermore, in patients with tuberculous pleuritis, proliferative responses to the 10-kDa Ag were higher in pleural fluid mononuclear cells than in PBMC, indicating that T cell reactivity to this Ag is enhanced at the site of disease. The first 15 amino acids of the 10-kDa Ag were identical to those defined previously for Bacillus Calmette-Guérin-a (BCG-a), and a T cell clone recognized the 10-kDa Ag and a peptide of BCG-a, indicating that the 10-kDa Ag corresponds to BCG-a. This Ag elicited IFN-gamma production by pleural fluid mononuclear cells and by PBMC from healthy tuberculin reactors, suggesting that the 10-kDa Ag can enhance macrophage activation and resistance to mycobacterial infection. Our findings indicate that the 10-kDa Ag of M. tuberculosis is highly immunoreactive and should be evaluated for its capacity to elicit protective immunity. PMID:1371791

Barnes, P F; Mehra, V; Rivoire, B; Fong, S J; Brennan, P J; Voegtline, M S; Minden, P; Houghten, R A; Bloom, B R; Modlin, R L




Microsoft Academic Search

RESUMO: O trabalho tem como principal objetivo estimar as constantes elásticas da madeira, empre- gando-se uma metodologia não destrutiva, de ondas ultra-sonoras (ultra-som). Foram utilizadas madei- ras de quatro espécies: Fava Amargosa (Vataireopsis speciosa), Jequitiba Rosa (Cariniana micran- tha), Peroba d'Água (Rauwolfia paraensis) e Peroba Mico (Aspidosperma macrocarpum). Por meio da determinação das velocidades de propagação das ondas ultra-sonoras, baseando-se

Joaquim Carlos Gonçalez; Ailton Teixeira


In vitro degradation of the 32kDa PS II reaction centre protein  

SciTech Connect

The 32kDa thylakoid membrane protein is an integral component of the PS II reaction centre. The protein, although stable in the dark, undergoes light dependent turnover. Light from the UV, visible and far-red spectral regions induce 32kDa protein degradation. To better understand 32kDa protein metabolism, an in vitro degradation system is being developed. It consists of isolated thylakoid membranes than contain radiolabelled protein. The 32kDa protein is actively and specifically degraded when the thylakoid preparation is exposed to UV or visible radiation. The protein is stable in the dark. The herbicides (atrazine and DCMU) inhibit degradation in the in vitro system as they do in vivo. Additionally, several methods of isolating thylakoids are being compared to optimize the 32kDa protein degradation reaction. The preparations will be evaluated based on their ability to permit light dependent degradation of the 32kDa protein without affecting the other membrane components.

Eckenswiller, L.C.; Greenberg, B.M. (Univ. of Waterloo, Ontario (Canada))



OpenDA: Open Source Generic Data Assimilation Environment and its Application in Geophysical Process Models  

NASA Astrophysics Data System (ADS)

Data Assimilation techniques are essential elements in state-of-the-art development of models and their use in operational forecasting and real-time control in the fields of groundwater, surface water and soil systems. In meteorological and atmospheric sciences, steady improvements in numerical weather forecasting and climate prediction over the last couple of decades have been enabled to a large degree by the development of community-based models and data assimilation systems. The hydrologic community should learn from the experiences of the meteorological and atmospheric communities by accelerating the transition of hydrologic DA research into operations and developing community-supported, open-source modeling and forecasting systems and data assimilation tools. In 2010, a community based open source initiative named OpenDA was started. The openDA initiative bears similarities with the well-known openMI initiative. OpenDA makes use of a set of interfaces that describe the interaction between models, observations and data assimilation algorithms. It focuses on flexible applications in portable systems for modeling geophysical processes. It provides a generic interfacing protocol that allows combination of the implemented data assimilation techniques with, in principle, any time-stepping model describing a process (atmospheric processes, 3D circulation, 2D water flow, rainfall-runoff, unsaturated flow, groundwater flow, etc.). Presently, openDA features filtering techniques and calibration techniques. The presentation will give an overview of openDA and the results of some of its practical applications.

Weerts, A.; van Velzen, N.; Verlaan, M.; Sumihar, J.; Hummel, S.; El Serafy, G.; Dhondia, J.; Gerritsen, H.; Vermeer-Ooms, S.; Loots, E.; Markus, A.; Kockx, A.



A novel 550-kDa protein in skeletal muscle of chick embryo: purification and localization.  


We have found a novel protein with a molecular mass of 550 kDa on SDS-polyacrylamide gels, which is abundant in skeletal muscle tissues at an early stage of chick embryonic development. The 550-kDa protein decreased with the progress of development, and only a slight amount of the protein was present in adult chicken skeletal muscle. The 550-kDa protein was purified from the cytoplasm of 18 day embryos by a procedure including ultracentrifugation and gel filtration. The purified 550-kDa protein was essentially free of contaminants as judged by SDS-PAGE. By immunofluorescence and immunoelectron microscopy using the antibody raised against the 550-kDa protein, this protein was shown to be localized in the peripheries of adult muscle fibers and at the Z-disks of isolated myofibrils. These findings have led us to conclude that the 550-kDa protein is a novel myofibrillar protein in chicken skeletal muscle. PMID:7492577

Hattori, A; Wakamatsu, J; Ishii, T; Kuwahara, K; Tatsumi, R



The sandfly fauna (Diptera: Psychodidae: Phlebotominae) of the Parque Estadual da Serra da Tiririca, Rio de Janeiro, Brazil  

PubMed Central

Cutaneous leishmaniasis (CL) in the state of Rio de Janeiro is sporadic and can be characterised as a peridomestic transmission that occurs in modified natural environments. The aim of this work was to study the fauna and ecological characteristics of sandflies in an environmentally protected area (the State Park of Serra da Tiririca) within the remnants of the Atlantic Forest in the municipalities of Niterói and Maricá and their possible relationship with leishmaniasis. Captures were performed using light traps during the night once a month for one year in both sylvatic environments and areas surrounding homes near the park. A total of 1,037 sandflies were captured, belonging to nine genera and 12 species: Evandromyia tupynambai (34.1%), Migonemyia migonei (20.6%), Brumptomyia cunhai (13.8%), Micropygomyia schreiberi (9.7%), Psathyromyia lanei (6.5%), Brumptomyia nitzulescui (5.7%), Evandromyia edwardsi (5.4%), Nyssomyia intermedia (2.8%), Evandromyia cortelezzii (0.6%), Pintomyia bianchigalatiae (0.5%), Lutzomyia longipalpis (0.2%) and Sciopemyia microps (0.1%). Both Mg. migonei and Ny. intermedia may be acting as vectors of CL in this area.

Rodrigues, Andressa Alencastre Fuzari; Barbosa, Vanessa de Araujo; Andrade, Jose Dilermando; Brazil, Reginaldo Pecanha



D-amphetamine (A)-induced dopamine (DA) release is not strictly dependent on newly-synthesized transmitter  

SciTech Connect

A is thought to exert its stimulant effects by releasing DA from a newly synthesized transmitter pool. This hypothesis was evaluated directly by measuring the basal efflux and electrically-evoked release of endogenous DA and dihydroxyphenylacetic acid (DOPAC). In striatal slices from reserpine-treated rabbits A increased DA efflux, reduced DOPAC efflux, and inhibited electrically-evoked /sup 3/H-ACh release in a concentration-dependent manner. These effects could not be mimicked by inhibition of neuronal uptake or MAO, but were blocked by inhibition of DA synthesis or neuronal uptake, and were potentiated by inhibition of MAO. In slices with intact vesicular transmitter stores A induced DA efflux was 2-fold greater than that seen in slices having no vesicular stores. Inhibition of DA synthesis reduced A-induced DA efflux by 60%, but had little effect on the ability of A to inhibit /sup 3/H-ACh release. A also increased the electrical stimulation-evoked overflow of DA (an effect which was attenuated slightly by synthesis inhibition), and potently inhibited DOPAC overflow. These results suggest that: 1) A facilitates efflux of axoplasmic DA by an accelerated exchange diffusion mechanism. The releasable axoplasmic pool is derived from newly synthesized and vesicular transmitter pools; 2) postsynaptic indices of transmitter release may be misleading; and 3) A increases electrically-evoked DA release possibly by inhibiting neuronal uptake.

Parker, E.; Cubeddu, L.



Role of Fluosol-DA 20% in prehospital resuscitation.  


Fluosol-DA 20% (FDA-20) resuscitation has been proposed for prehospital therapy of hemorrhagic shock (HS). Acute HS (mean arterial pressure 60 mm Hg X 90 min, then 40 mm Hg X 30 min) in 24 splenectomized dogs was treated with 50 ml/kg of lactated Ringer's solution (RL) plus a volume equal to 105% of shed blood volume of FDA-20 (group 1), FDA-20 carrier Annex solution (group 2), or RL (group 3). Cardiopulmonary, hemopoietic, hemodynamic, and organ function parameters were measured preshock, at the end of shock, and at 30 and 60 min, and 24 h after resuscitation. FDA-20 produced effective volume expansion, oxygen delivery, and oxygen consumption. The FDA-20 appeared to affect organ function and cells adversely as reflected by a fall in red cell mass and platelet levels and by a rise in liver enzymes, BUN, and serum creatinine. Both the FDA-20 and Annex solution led to a reduction in serum proteins including serum albumin, serum globulin, immunoglobulin-G, and fibrinogen. These reductions are probably due to an oncotically driven factor which appears to maintain an optimal lymph to plasma oncotic ratio. The hydroxyethyl starch in the FDA-20 and the Annex solution, thus, would drive the plasma proteins into the interstitial space. The prolonged prothrombin times and the activated partial thromboplastin times after FDA-20 may be due, in part, to a reduction in the coagulation proteins, although these were not measured. Pending further studies designed to assess the effects of FDA-20 on possible cellular and multiple organ toxicity, clinical studies are premature and unwarranted. PMID:2914450

Elliott, L A; Ledgerwood, A M; Lucas, C E; McCoy, L E; McGonigal, M; Sullivan, M W



ALTERAÇÕES DA FASE ORAL DA DEGLUTIÇÃO EM CRIANÇAS COM PARALISIA CEREBRAL Changes in the oral phase of swallowing in children with cerebral palsy  

Microsoft Academic Search

RESUMO Objetivos: Verificar as alterações na fase oral da deglutição em crianças com paralisia cerebral. Método: Foi conduzido um estudo quantitativo, observacional e transversal, desenvolvido no período de junho a agosto de 2006 no NAMI - Núcleo de Atenção Médica Integrada, em Fortaleza-CE. A amostra constituiu-se de 20 crianças na faixa etária de 1 ano e 6 meses a 8

Karise Santos Vasconcelos



The 37-kDa/67-kDa laminin receptor acts as the cell-surface receptor for the cellular prion protein  

PubMed Central

Recently, we identified the 37-kDa laminin receptor precursor (LRP) as an interactor for the prion protein (PrP). Here, we show the presence of the 37-kDa LRP and its mature 67-kDa form termed high-affinity laminin receptor (LR) in plasma membrane fractions of N2a cells, whereas only the 37-kDa LRP was detected in baby hamster kidney (BHK) cells. PrP co-localizes with LRP/LR on the surface of N2a cells and Semliki Forest virus (SFV) RNA transfected BHK cells. Cell-binding assays reveal the LRP/LR-dependent binding of cellular PrP by neuronal and non-neuronal cells. Hyperexpression of LRP on the surface of BHK cells results in the binding of exogenous PrP. Cell binding is similar in PrP+/+ and PrP0/0 primary neurons, demonstrating that PrP does not act as a co-receptor of LRP/LR. LRP/LR-dependent internalization of PrP is blocked at 4°C. Secretion of an LRP mutant lacking the transmembrane domain (aa 86–101) from BHK cells abolishes PrP binding and internalization. Our results show that LRP/LR acts as the receptor for cellular PrP on the surface of mammalian cells.

Gauczynski, Sabine; Peyrin, Jean-Michel; Haik, Stephane; Leucht, Christoph; Hundt, Christoph; Rieger, Roman; Krasemann, Susanne; Deslys, Jean-Philippe; Dormont, Dominique; Lasmezas, Corinne Ida; Weiss, Stefan



Comparison of PLS1DA, PLS2DA and SIMCA for classification by origin of crude petroleum oils by MIR and virgin olive oils by NIR for different spectral regions  

Microsoft Academic Search

This study compares results obtained with several chemometric methods: SIMCA, PLS2-DA, PLS2-DA with SIMCA, and PLS1-DA in two infrared spectroscopic applications. The results were optimized by selecting spectral ranges containing discriminant information. In the first application, mid-infrared spectra of crude petroleum oils were classified according to their geographical origins. In the second application, near-infrared spectra of French virgin olive oils

O. Galtier; O. Abbas; Y. Le Dréau; C. Rebufa; J. Kister; J. Artaud; N. Dupuy



NADH:ubiquinone oxidoreductase from bovine heart mitochondria. cDNA sequences of the import precursors of the nuclear-encoded 39 kDa and 42 kDa subunits.  

PubMed Central

The 39 kDa and 42 kDa subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria are nuclear-coded components of the hydrophobic protein fraction of the enzyme. Their amino acid sequences have been deduced from the sequences of overlapping cDNA clones. These clones were amplified from total bovine heart cDNA by means of the polymerase chain reaction, with the use of complex mixtures of oligonucleotide primers based upon fragments of protein sequence determined at the N-terminals of the proteins and at internal sites. The protein sequences of the 39 kDa and 42 kDa subunits are 345 and 320 amino acid residues long respectively, and their calculated molecular masses are 39,115 Da and 36,693 Da. Both proteins are predominantly hydrophilic, but each contains one or two hydrophobic segments that could possibly be folded into transmembrane alpha-helices. The bovine 39 kDa protein sequence is related to that of a 40 kDa subunit from complex I from Neurospora crassa mitochondria; otherwise, it is not related significantly to any known sequence, including redox proteins and two polypeptides involved in import of proteins into mitochondria, known as the mitochondrial processing peptidase and the processing-enhancing protein. Therefore the functions of the 39 kDa and 42 kDa subunits of complex I are unknown. The mitochondrial gene product, ND4, a hydrophobic component of complex I with an apparent molecular mass of about 39 kDa, has been identified in preparations of the enzyme. This subunit stains faintly with Coomassie Blue dye, and in many gel systems it is not resolved from the nuclearcoded 36 kDa subunit. Images Fig. 1.

Fearnley, I M; Finel, M; Skehel, J M; Walker, J E



Nucleus-Localized 21.5-kDa Myelin Basic Protein Promotes Oligodendrocyte Proliferation and Enhances Neurite Outgrowth in Coculture, Unlike The Plasma Membrane-Associated 18.5-kDa Isoform  

PubMed Central

The classic myelin basic protein (MBP) family of central nervous system (CNS) myelin arises from transcription start site 3 of the Golli (gene of oligodendrocyte lineage) complex and comprises splice isoforms ranging in nominal molecular mass from 14 kDa to (full-length) 21.5 kDa. We have determined here a number of distinct functional differences between the major 18.5-kDa and minor 21.5-kDa isoforms of classic MBP with respect to oligodendrocyte (OLG) proliferation. We have found that, in contrast to 18.5-kDa MBP, 21.5-kDa MBP increases proliferation of early developmental immortalized N19-OLGs by elevating the levels of phosphorylated ERK1/2 and Akt1 kinases and of ribosomal protein S6. Coculture of N2a neuronal cells with N19-OLGs transfected with the 21.5-kDa isoform (or conditioned medium from), but not the 18.5-kDa isoform, caused the N2a cells to have increased neurite outgrowth and process branching complexity. These roles were dependent on subcellular localization of 21.5-kDa MBP to the nucleus and on the exon II-encoded segment, suggesting that the nuclear localization of early minor isoforms of MBP may play a crucial role in regulating and/or initiating myelin and neuronal development in the mammalian CNS.

Smith, Graham S.T.; Samborska, Bozena; Hawley, Steven P.; Klaiman, Jordan M.; Gillis, Todd E.; Jones, Nina; Boggs, Joan M.; Harauz, George



An Introduction to the DA-T Gibbs Sampler for the Two-Parameter Logistic (2PL) Model and beyond  

ERIC Educational Resources Information Center

The DA-T Gibbs sampler is proposed by Maris and Maris (2002) as a Bayesian estimation method for a wide variety of "Item Response Theory (IRT) models". The present paper provides an expository account of the DA-T Gibbs sampler for the 2PL model. However, the scope is not limited to the 2PL model. It is demonstrated how the DA-T Gibbs sampler for…

Maris, Gunter; Bechger, Timo M.



Percepção Astronômica de Alunos do Ensino Médio da Rede Estadual de São Paulo  

NASA Astrophysics Data System (ADS)

Embora a astronomia seja uma das ciências mais antigas da humanidade e muitos dos conceitos astronômicos serem populares, principalmente nesta época de alta globalização do conhecimento por intermédio de eficientes meios de comunicação e de obtenção da informação, notadamente através da internet, observa-se que uma parcela significativa dos estudantes encontra-se à margem dessas informações. O presente trabalho visa analisar o nível de conhecimento básico dos alunos de Ensino Médio da rede estadual da cidade de Suzano quanto aos fenômenos astronômicos que os rodeiam, tais como a sucessão dos dias e das estações do ano, além de questioná-los sobre fatos genéricos tais como: quais são os astros que se encontram mais próximos do planeta Terra, o que vem a ser o Sol, o Big Bang, um ano-luz, uma estrela cadente, a estrela de Bélem e o que ocasionou a extinção dos dinossauros. Para tanto foi elaborado um formulário constando de questões de múltipla escolha, o qual foi aplicado no primeiro colegial noturno da Escola Estadual Batista Renzi. Num espaço amostral de 34 alunos constatou-se que apenas 29,4% compreendiam a sucessão dos dias da semana, que apenas 20,6% explicaram corretamente as estações do ano, que apenas 20,6% tinham idéia de quais são os objetos celestes mais próximos da Terra, em contraposição 67,6% sabiam classificar corretamente o Sol como estrela, 55,9% relacionavam o Big Bang à origem do universo, apenas 20,6% identificavam um ano-luz como unidade de distância, 32,4% reconheciam uma estrela cadente como meteoro, 41,2% consideravam a estrela de Belém como um cometa e 50,0% explicaram corretamente a extinção dos dinossauros. A presente análise será expandida para as demais classes de primeiro colegial, não somente do período noturno, mas também do diurno da Escola Estadual Batista Renzi, bem como o formulário será devidamente ampliado. Já nesta primeira fase nota-se claramente o pequeno discernimento de eventos astronômicos dos alunos e principalmente a grande confusão sobre o significado correto de termos astronômicos populares.

de Oliveira, E. F.; Voelzke, M. R.; Amaral, L. H.



A constitutively expressed 36 kDa exochitinase from Bacillus thuringiensis HD-1.  


A 36 kDa chitinase was purified by ion exchange and gel filtration chromatography from the culture supernatant of Bacillus thuringiensis HD-1. The chitinase production was independent of the presence of chitin in the growth medium and was produced even in the presence of glucose. The purified chitinase was active at acidic pH, had an optimal activity at pH 6.5, and showed maximum activity at 65 degrees C. Of the various substrates, the enzyme catalyzed the hydrolysis of the disaccharide 4-MU(GlnAc)(2) most efficiently and was therefore classified as an exochitinase. The sequence of the tryptic peptides showed extensive homology with Bacillus cereus 36 kDa exochitinase. The 1083 bp open reading frame encoding 36 kDa chitinase was amplified with primers based on the gene sequence of B. cereus 36 kDa exochitinase. The deduced amino-acid sequence showed that the protein contained an N-terminal signal peptide and consisted of a single catalytic domain. The two conserved signature sequences characteristic of family 18 chitinases were mapped at positions 105-109 and 138-145 of Chi36. The recombinant chitinase was expressed in a catalytically active form in Escherichia coli in the vector pQE-32. The expressed 36 kDa chitinase potentiated the insecticidal effect of the vegetative insecticidal protein (Vip) when used against neonate larvae of Spodoptera litura. PMID:12893268

Arora, Naresh; Ahmad, Tarannum; Rajagopal, R; Bhatnagar, Raj K



mUbiSiDa: A Comprehensive Database for Protein Ubiquitination Sites in Mammals  

PubMed Central

Motivation Protein ubiquitination is one of the important post-translational modifications by attaching ubiquitin to specific lysine (K) residues in target proteins, and plays important regulatory roles in many cell processes. Recent studies indicated that abnormal protein ubiquitination have been implicated in many diseases by degradation of many key regulatory proteins including tumor suppressor, oncoprotein, and cell cycle regulator. The detailed information of protein ubiquitination sites is useful for scientists to investigate the mechanism of many cell activities and related diseases. Results In this study we established mUbiSida for mammalian Ubiquitination Site Database, which provides a scientific community with a comprehensive, freely and high-quality accessible resource of mammalian protein ubiquitination sites. In mUbiSida, we deposited about 35,494 experimentally validated ubiquitinated proteins with 110,976 ubiquitination sites from five species. The mUbiSiDa can also provide blast function to predict novel protein ubiquitination sites in other species by blast the query sequence in the deposit sequences in mUbiSiDa. The mUbiSiDa was designed to be a widely used tool for biologists and biomedical researchers with a user-friendly interface, and facilitate the further research of protein ubiquitination, biological networks and functional proteomics. The mUbiSiDa database is freely available at

He, Bing; Yu, Haiyan; Guo, Xuejiang; Song, Xiaofeng; Sha, Jiahao



Audio watermarking robust against D/A and A/D conversions  

NASA Astrophysics Data System (ADS)

Digital audio watermarking robust against digital-to-analog (D/A) and analog-to-digital (A/D) conversions is an important issue. In a number of watermark application scenarios, D/A and A/D conversions are involved. In this article, we first investigate the degradation due to DA/AD conversions via sound cards, which can be decomposed into volume change, additional noise, and time-scale modification (TSM). Then, we propose a solution for DA/AD conversions by considering the effect of the volume change, additional noise and TSM. For the volume change, we introduce relation-based watermarking method by modifying groups of the energy relation of three adjacent DWT coefficient sections. For the additional noise, we pick up the lowest-frequency coefficients for watermarking. For the TSM, the synchronization technique (with synchronization codes and an interpolation processing operation) is exploited. Simulation tests show the proposed audio watermarking algorithm provides a satisfactory performance to DA/AD conversions and those common audio processing manipulations.

Xiang, Shijun



Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes  

PubMed Central

This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity was assessed by western blot analysis with patients' sera. The mAbs against the 100 kDa antigen commonly reacted with various kinds of trematode antigens, including intestinal (Gymnophalloides seoi), lung (Paragonimus westermani), and liver flukes (Clonorchis sinensis and Fasciola hepatica). However, this mAb showed no cross-reactions with other helminth parasites, including nematodes and cestodes. To determine the topographic distribution of the 100 kDa antigen in worm sections, indirect immunoperoxidase staining was performed. A strong positive reaction was observed in the tegumental and subtegumental layers of adult M. yokogawai and C. sinensis. The results showed that the 100 kDa somatic protein of M. yokogawai is a common antigen which recognizes a target epitope present over the tegumental layer of different trematode species.

Park, Young-Jin; Park, Jeong-Hyun; Chai, Jong-Yil



Metagonimus yokogawai: a 100-kDa Somatic Antigen Commonly Reacting with Other Trematodes.  


This study was undertaken to characterize the properties of a 100 kDa somatic antigen from Metagonimus yokogawai. Monoclonal antibodies (mAbs) were produced against this 100 kDa antigen, and their immunoreactivity was assessed by western blot analysis with patients' sera. The mAbs against the 100 kDa antigen commonly reacted with various kinds of trematode antigens, including intestinal (Gymnophalloides seoi), lung (Paragonimus westermani), and liver flukes (Clonorchis sinensis and Fasciola hepatica). However, this mAb showed no cross-reactions with other helminth parasites, including nematodes and cestodes. To determine the topographic distribution of the 100 kDa antigen in worm sections, indirect immunoperoxidase staining was performed. A strong positive reaction was observed in the tegumental and subtegumental layers of adult M. yokogawai and C. sinensis. The results showed that the 100 kDa somatic protein of M. yokogawai is a common antigen which recognizes a target epitope present over the tegumental layer of different trematode species. PMID:24850966

Han, Eun-Taek; Yang, Hyun-Jong; Park, Young-Jin; Park, Jeong-Hyun; Chai, Jong-Yil



Effects of ?-Da1a a peptidic ?1A-adrenoceptor antagonist in human isolated prostatic adenoma and anaesthetized rats  

PubMed Central

Background and Purpose ?-Da1a, a 65 amino-acid peptide, has subnanomolar affinity and high selectivity for the human ?1A-adrenoceptor subtype. The purpose of this study was to characterize the pharmacological effects of ?-Da1a on prostatic function, both in vivo and in vitro. Experimental Approach ?-Da1a was tested as an antagonist of adrenaline-induced effects on COS cells transfected with the human ?1A-adrenoceptor as well as on human isolated prostatic adenoma obtained from patients suffering from benign prostatic hyperplasia. Moreover, we compared the effects of ?-Da1a and tamsulosin on phenylephrine (PHE)-induced increases in intra-urethral (IUP) and arterial pressures (AP) in anaesthetized rats, following i.v. or p.o. administration. Key Results On COS cells expressing human ?1A-adrenoceptors and on human prostatic strips, ?-Da1a inhibited adrenaline- and noradrenaline-induced effects. In anaesthetized rats, ?-Da1a and tamsulosin administered i.v. 30 min before PHE significantly antagonized the effects of PHE on IUP. The pKB values for tamsulosin and ?-Da1a for this effect were similar. With regards to AP, ?-Da1a only reduced the effect of PHE on AP at the lowest dose tested (10 ?g·kg?1), whereas tamsulosin significantly reduced PHE effects at doses between 10 and 150 ?g·kg?1. Conclusions and Implications ?-Da1a exhibited a relevant effect on IUP and a small effect on AP. In contrast, tamsulosin antagonized the effects of PHE on both IUP and AP. We conclude that ?-Da1a is more uroselective than tamsulosin. ?-Da1a is the most selective peptidic antagonist for ?1A-adenoceptors identified to date and could be a new treatment for various urological diseases.

Palea, S; Maiga, A; Guilloteau, V; Rekik, M; Guerard, M; Rouget, C; Rischmann, P; Botto, H; Camparo, P; Lluel, P; Gilles, N



Identification of an abundant 56 kDa protein implicated in food allergy as granule-bound starch synthase.  


Rice, the staple food of south and east Asian counties, is considered to be hypoallergenic. However, several clinical studies have documented rice-induced allergy in sensitive patients. Rice proteins with molecular weights of 14-16, 26, 33, and 56 kDa have been identified as allergens. Recently, it was documented that the 56 kDa rice allergen was responsible for rice-induced anaphylaxis. The 14-16 kDa allergens have been identified as ?-amylase inhibitors; the 26 kDa protein has been identified as ?-globulin; and the 33 kDa protein has been identified as glyoxalase I. However, the identity of the 56 kDa rice allergen has not yet been determined. In this study, we demonstrate that serum from patients allergic to maize shows IgE binding to a 56 kDa protein that was present in both maize and rice but not in the oil seeds soybean and peanut. The 56 kDa IgE-binding protein was abundant in the rice endosperm. We have purified this protein from rice endosperm and demonstrated its reactivity to IgE antibodies from the serum of maize-allergic patients. The purified protein was subjected to matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry analysis, resulting in identification of this rice allergen as granule-bound starch synthase, a product of the Waxy gene. Immunoblot analysis using protein extracts from a waxy mutant of rice revealed the absence of the 56 kDa IgE-binding protein. Our results demonstrate that the 56 kDa rice allergen is granule-bound starch synthase and raise the possibility of using waxy mutants of rice as a potential source of the hypoallergenic diet for patients sensitized to the 56 kDa rice allergen. PMID:23675783

Krishnan, Hari B; Chen, Ming-Hsuan




Microsoft Academic Search

A busca por um ensino de física que desperte o senso crítico de alunos e professore s sobre assuntos relacionados à C iência tem promovido a criação de diversas propostas e iniciativas que envolvam a discussão da Natureza da Ciência (NdC). A NdC pode ser definida como um conjunto de saberes sobre a C iência e sobre os processos que

Breno Arsioli Moura; Cibelle Celestino Silva



Solution Structure of the 128 kDa Enzyme I Dimer from Escherichia coli and Its 146 kDa Complex with HPr Using Residual Dipolar Couplings and Small and Wide-Angle X-ray Scattering  

Microsoft Academic Search

The solution structures of free Enzyme I (EI, 128 kDa, 575 x 2 residues), the first enzyme in the bacterial phosphotransferase system, and its complex with HPr (146 kDa) have been solved using novel methodology that makes use of prior structural knowledge (namely, the structures of the dimeric EIC domain and the isolated EIN domain both free and complexed to

Charles D. Schwieters; Jeong-Yong Suh; Alexander Grishaev; Rodolfo Ghirlando; Yuki Takayama; G. Marius Clore



Spectroscopy of the DA white dwarfs - Automatic atmospheric parameterization and mass distribution  

NASA Technical Reports Server (NTRS)

A method for the automatic calculation of the atmospheric parameters (Teff and log g) of hydrogen-rich degenerate stars from low-resolution spectra is described, and then applied to the spectra of 53 DA white dwarfs. A value for the width of the DA mass distribution of sigma M/solar-M not greater than +0.10 is obtained using the proposed approach. The data indicate that the distribution is asymmetrically skewed to low masses; however, there is also evidence of a high-mass non-Gaussian tail.

Mcmahan, Robert K.



DAEdALUS: A New Search for CP Violation in the Neutrino Sector  

NASA Astrophysics Data System (ADS)

The DAEdALUS experiment offers a novel approach to measuring CP violation in the neutrino sector. The design uses multiple cyclotrons to produce intense beams of neutrinos from the decay-at-rest of pions and muons. Short-baseline muon- to electron-antineutrino oscillations are observed via inverse beta decay in an ultra-large water Cerenkov detector at DUSEL. The experiment's high-statistics, low-background output yields a CP violation sensitivity competitive with that of LBNE. Together, the complementary designs of DAEdALUS and LBNE provide the highest sensitivity of any presently proposed CP violation search.

Carr, Rachel



Intervenções em consciência fonológica e aprendizagem da linguagem escrita 1 Interventions in phonological conscience and learning of the written language  

Microsoft Academic Search

Resumo: Vários estudos evidenciam alta associação entre a consciência fonológica e as habilidades de ler e escrever. Este trabalho tem como objetivo identificar e analisar pesquisas de intervenção em consciência fonológica e seus efeitos sobre a aprendizagem da linguagem escrita. São identificados três tipos de pesquisas: com adultos analfabetos, com crianças em dificuldade na aprendizagem da escrita e com pré-escolares.

Maria José dos Santos


Modello di valutazione dell'attitudine fisica del territorio per la realizzazione di impianti cedui da biomassa in Italia  

Microsoft Academic Search

Le piantagioni governate a ceduo a turno breve (short rotation forestry - SRF) rappresentano il modello di arboricoltura da legno maggiormente idoneo alla produzione di biomassa lignocellulosica da destinare a usi energetici. La limitata disponibilità di risorse e la rete sempre più complessa di relazioni che coinvolgono l'uso del territorio pon- gono l'esigenza di una attenta programmazione territoriale di questo

Riccardo Salvati; Gherardo Chirici; Piermaria Corona



Avaliação das normas de biossegurança nas clínicas odonto- lógicas da UFPE Evaluation of biosecutrity norms in UFPE dental clinics  

Microsoft Academic Search

Key-words O objetivo deste trabalho foi observar o cumprimento das normas de biossegurança e os cuidados com os riscos ocupacionais pelos alunos que atenderam nas clínicas de Odontologia Preventiva, Odontopediatria, Dentística, Endodontia e nos procedimentos cirúrgicos nas clínicas de Periodontia e Cirurgia, da Faculdade de Odontologia da Universidade Federal de Pernambuco. Este trabalho consistiu em um estudo observacional, no qual

Márcia Maria; Vendiciano Barbosa Vasconcelos; Catarina da Mot; Vasconcelos Brasi; Cláudia Cristina; Brainer de Oliveira Mot; Rabelo de Carvalho


Induction of fibroblast 92 kDa gelatinase/type IV collagenase expression by direct contact with metastatic tumor cells.  


Previous studies have correlated release of the 92 kDa type IV collagenase/gelatinase by tumor cells in culture with metastatic potential. We have now demonstrated that the ability of tumor cells that do not express the 92 kDa gelatinase to induce release of this metalloproteinase from normal fibroblasts may also be associated with the metastatic phenotype. A transformed rat embryo cell line, 2.8, failed to release the 92 kDa gelatinase alone in culture, but gave rise to metastatic tumors whose explants contained the 92 kDa gelatinase. In contrast, a non-metastatic transformed cell line, RA3, did not express the 92 kDa gelatinase alone in culture or in tumor explants. To explore the mechanisms that might govern host-tumor cell interactions in this system, we have studied the effects of co-culture of these transformed cell lines with rat embryo fibroblasts (REF) in culture. 92 kDa gelatinase expression was induced by co-culture of 2.8 with REF, but co-culture of the non-metastatic line RA3 with REF did not result in induction of the 92 kDa gelatinase. The 92 kDa gelatinase in these co-cultures was released by the fibroblasts; methanol-fixed 2.8 cells induced 92 kDa gelatinase expression in REF, but fixed REF cells did not induce enzyme expression in 2.8 cells. This suggested that cell contact was required for induction, which was confirmed by showing that 92 kDa gelatinase induction in co-culture was abolished by separating REF from 2.8 by solute-permissive membranes. In addition, REF could not be stimulated to produce the 92 kDa gelatinase by 2.8-derived conditioned medium, by 2.8-derived extracellular matrix, or by isolated matrix components. These data indicate that metastatic tumor cells can induce 92 kDa gelatinase expression in fibroblasts through a mechanism dependent upon cell contact. In situ hybridization of nude mouse tumors derived from these transformed cell lines revealed 92 kDa gelatinase expression in the stroma of tumors from 2.8, but not in tumors from RA3. Therefore, the experiments based on in vitro co-culture of tumor cells and fibroblasts, together with the in situ localization of mRNA to host cells, suggest that host production of the 92 kDa gelatinase may occur in response to direct contact with metastatic tumor cells. PMID:8207074

Himelstein, B P; Canete-Soler, R; Bernhard, E J; Muschel, R J



A 23-kDa protein as a substrate for protein kinase C in bovine neutrophils. Purification and partial characterization  

SciTech Connect

In {sup 32}P{sub i}-loaded bovine neutrophils stimulated with phorbol myristate acetate (PMA), radioactivity was preferentially incorporated into a protein of low molecular mass, suggesting a PKC-dependent phosphorylation. This protein, termed 23-kDa protein, was predominantly localized in the cytosol. The apparent molecular mass of the purified protein range between 20 and 23 kDa. In the absence of mercaptoethanol, a dimer accumulated. Homogeneity of the 23-kDa protein was verified by 2D-PAGE analysis. Gel isoelectric focusing (IEF) of the purified 23-kDa protein followed by Coomassie blue staining allowed the visualization of our discrete protein bands with isoelectric points ranging between pH 6.3 and 6.7. Phosphorylation of the 23-kDa protein by ({gamma}-{sup 32}P)ATP in the presence of bovine neutrophil PKC supplemented with Ca{sup 2+}, phosphatidylserine, and diacylglycerol or with PMA occurred on serine and required the presence of mercaptoethanol. IEF of the {sup 32}P-labeled 23-kDa protein followed by autoradiography revealed for discrete bands with distinct isoelectric points similar to those of the bands stained by Coomassie blue after IEF on nonlabeled 23-kDa protein. The bands of the 23-kDa protein resolved by IEF and transfered to nitrocellulose showed ability to bind ({sup 35}S)GTP-{gamma}-S. The immunoreactivity of antibodies raised in rabbits against the bovine neutrophil 23-kDa protein was demonstrated on immunoblots after SDS-PAGE. The 23-kDa protein differed also from several other proteins of similar molecular mass that have been identified in neutrophils, namely, calmodulin, the small subunit of the low-potential cytochrome b, and a low molecular weight protein which is ADP-ribosylated by the botulinum toxin.

Stasia, M.J.; Dianoux, A.C.; Vignais, P.V. (Centre d'Etudes Nucleaires (France))



Fermentação da proteína de seis alimentos por microrganismos ruminais, incubados puros ou com monensina ou rumensin®  

Microsoft Academic Search

RESUMO - Avaliaram-se os efeitos da fermentação in vitro de seis alimentos: fubá de milho (FM), farelo de soja (FS), farelo de trigo (FT), sorgo (SO), glúten de milho (GM) e uréia (UR), incubados puros ou com o antibiótico monensina, esse na forma pur a para análise (Monensina) ou comercial (Rumensin®). O experimento constituiu-se de 18 unidades experimentais (três alimentos

Natália Guarino Souza Barbosa; Rogério de Paula Lana; Antônio Bento Mâncio; Arnaldo Chaer Borges; Cesar de Queiroz; Juliana Silva Oliveira



O outro pé da sereia: História[s] na pós-colonialidade  

Microsoft Academic Search

This article discusses Mia Couto's novel O outro pé da sereia (2006) in terms of the novel's significant readings of Mozambican history, tradition and cultural context. Postcolonial theory provides a critical framework for the study of questions that appear in the novel and represent a set of issues that are central to both Mia Couto's work and to the broader

Elena Brugioni



Influência do veículo na eficácia da reposição de potássio em ratos hipocalêmicos  

Microsoft Academic Search

Resumo Introdução: Pacientes após cirurgia cardíaca são comumente tratados com diuréticos para controle de volume plasmático. A preocupação de distúrbios hipocalêmicos em adultos antes, durante ou após a cirurgia já foi ressaltada anteriormente, visto o risco de arritmias cardíacas. Clinicamente, a diluição da solução de potássio (K+) para administração por via intravenosa, em situações que requerem a sua reposição é

Márcio Petenusso; Vitor Engrácia Valenti; Luiz Carlos de Abreu; Eduardo Colombari; Fernando Luiz Affonso Fonseca; Monica Akemi Sato



Migration from can coatings: Part 2. Identification and quantification of migrating cyclic oligoesters below 1000?Da  

Microsoft Academic Search

Metal cans for food use can be coated with lacquers based on polyester resins. Recent research has focussed on the identification and quantification of migrants released by coatings that are potentially absorbable (below 1000?Da). The presented method describes a procedure that was optimized to hydrolyse the polyester migrants into their monomers, polyvalent acids and polyols. The polyols were identified by

A. Schaefer; V. A. Ohm; T. J. Simat



32 CFR 644.512 - DA-SBA joint set-aside determination.  

Code of Federal Regulations, 2013 CFR

...CONTINUED) REAL PROPERTY REAL ESTATE HANDBOOK Disposal Disposal of Standing Timber, Crops, and Embedded Gravel, Sand and Stone § 644.512 DA-SBA joint set-aside determination. Section 15 of the Small Business Act (15 U.S.C. 644),...



Identification and isolation of a 155-kDa protein with neuropathy target esterase activity.  


A method is presented for the isolation of a 155-kDa protein that possesses phenyl valerate hydrolysis activity in the presence of paraoxon but is inhibited by mipafox; the functional definition of neuropathy target esterase (neurotoxic esterase; NTE). Microsomes, isolated from 18-day-old chicken embryos were treated with phospholipase A2 to solubilize the NTE activity. The extract was then combined with polyoxyethylene W1 detergent and resolved by gel filtration chromatography to yield an active fraction with an approximate mass of 200 kDa. This fraction was further purified by preparative isoelectric focusing and native electrophoresis to yield two separate bands possessing NTE activity. The slower migrating band was highly enriched in a 155-kDa protein that was identified as a source of the NTE activity by affinity chromatography using 3-(9'-mercaptononylthio)-1,1,1-trifluoro-propan-2-one bound to Sepharose CL6B. This represents the first report of the isolation of NTE in its active form and aids in the confirmation of the 155-kDa protein as the most likely candidate for NTE. PMID:8812209

Mackay, C E; Hammock, B D; Wilson, B W



Seasonal Mosquito Fluctuations in Da Nang, South Vietnam, Based on Three Prevalent Species.  

National Technical Information Service (NTIS)

A light trap survey was conducted in Da Nang, Vietnam for 42 weeks beginning 1 June 1969. Over 150,000 specimens were sorted and identified. The most numerous mosquitoes collected were C. tritaeniorhynchus, A. Sinensis, and A. vagus. C. tritaeniorhynchus ...

T. G. Floore R. H. Grothaus W. F. Miner



A Integracao de Ensino das Ciencias da Saude (An Integrated Medical Education Program [in Brazil]).  

ERIC Educational Resources Information Center

At the Sixth Annual Reunion of the Brazilian Association of Medical Schools (VI Reuniao Anual da Associacao Brasileira de Escolas Medicas) leaders in the Brazilian medical profession proposed an integrated educational program for training students in the fields of medicine and public health. Under Brazil's present system of education, all…

Pourchet-Campos, M. A.; Guimaraes Junior, Paulino


Long-term evolution of 1991 DA: A dynamically evolved extinct Halley-type comet  

NASA Astrophysics Data System (ADS)

The long-term dynamical evolution of 21 variational orbits for the intermediate-period asteroid 1991 DA was followed for up to +/-105 years from the present. 1991 DA is close to the 2:7 resonance with Jupiter; it has avoided close encounters, within 1 AU, with this planet for at least the past 30,000 years, even at the node crossing. The future evolution typically shows no close encounters with Jupiter within at least 50,000 years. This corresponds to the mean time between node crossings with either Jupiter or Saturn. Close encounters with Saturn and Jupiter lead to a chaotic evolution for the whole ensemble, while secular perturbations cause large-amplitude swings in eccentricity and inclination (the latter covering the range 15 deg approximately less than i approximately less than 85 deg) which correlate with deep excursions of the perihelion distance to values much less than 1 AU. These variations are similar to those found in P/Machholz and a variety of other high-inclination orbits, e.g., P/Hartley-IRAS. We emphasize the connection between the orbital evolution of 1991 DA and that of Halley-type comets. If 1991 DA was once a comet, it is not surprising that it is now extinct.

Hahn, Gerhard; Bailey, M. E.



Francis Bacon e as marés: a concepção da natureza e o mecanicismo  

Microsoft Academic Search

Esta breve introdução visa duas tarefas ao mesmo tempo: primeiro, informar sobre a época provável de composição e o lugar ocupado, no conjunto da obra de Francis Bacon (1561-1626), pelo pequeno tratado intitulado De fluxu et refluxu maris (Do fluxo e refluxo do mar); segundo, expor a solução que nele se apresenta para o problema das marés, mostrando tratar-se de

Pablo Rubén Mariconda



Precise Estimates of the Synthetic Spectral Distances to DA White Dwarfs  

Microsoft Academic Search

Using recent photometric calibrations, we develop greatly improved distance estimates for DA white dwarfs using multi-band synthetic photometry based on spectroscopic temperatures and gravities. Very good correlations are shown to exist between our spectroscopically based photometric distance estimates and those derived from trigonometric parallaxes. We investigate the uncertainties involved in our distance estimates, as well as discuss the circumstances where

J. B. Holberg; P. Bergeron; A. Gianninas



Preliminary Design Study of High-Power H2+ Cyclotrons for the DAEdALUS Experiment  

Microsoft Academic Search

This report provides a first design for H2+ accelerators as the DAEdALUS neutrino sources. A description of all aspects of the system, from the ion source to the extracted beam, is provided. The analysis provides a first proof of principle of a full cyclotron system which can provide the necessary beam power for the CP violation search proposed by the

L. Calabretta; L. Celona; S. Gammino; D. Rifuggiato; G. Ciavola; M. Maggiore; L. A. C. Piazza; J. R. Alonso; W. A. Barletta; A. Calanna; J. M. Conrad



Long-term evolution of 1991 DA: A dynamically evolved extinct Halley-type comet  

NASA Technical Reports Server (NTRS)

The long-term dynamical evolution of 21 variational orbits for the intermediate-period asteroid 1991 DA was followed for up to +/-10(exp 5) years from the present. 1991 DA is close to the 2:7 resonance with Jupiter; it has avoided close encounters, within 1 AU, with this planet for at least the past 30,000 years, even at the node crossing. The future evolution typically shows no close encounters with Jupiter within at least 50,000 years. This corresponds to the mean time between node crossings with either Jupiter or Saturn. Close encounters with Saturn and Jupiter lead to a chaotic evolution for the whole ensemble, while secular perturbations cause large-amplitude swings in eccentricity and inclination (the latter covering the range 15 deg approximately less than i approximately less than 85 deg) which correlate with deep excursions of the perihelion distance to values much less than 1 AU. These variations are similar to those found in P/Machholz and a variety of other high-inclination orbits, e.g., P/Hartley-IRAS. We emphasize the connection between the orbital evolution of 1991 DA and that of Halley-type comets. If 1991 DA was once a comet, it is not surprising that it is now extinct.

Hahn, Gerhard; Bailey, M. E.




EPA Science Inventory

Maternal Atrazine (ATR) alters hypothalamic dopamine (HYP-DA) and serum prolactin (sPRL) in male pups. 1Christopher Langdale, 2Tammy Stoker and 2Ralph Cooper. 1 Dept. of Cell Biology, North Carolina State University College of Veterinary Medicine, Raleigh, NC. 2 Endocrinology ...


Brominated and radioiodinated derivatives of methylphenidate (MP): Potential imaging agents for the dopamine (DA) transporter  

SciTech Connect

MP (Ritalin) is a psychomotor stimulant used in the treatment of attention-deficit hyperactivity disorder. The therapeutic properties of MP are thought to be mediated by its binding to a site on the DA transporter, resulting in inhibition of DA reuptake and enhanced levels of synaptic dopamine. MP also inhibits reuptake of norepinephrine (NE) in vitro. MP has two chiral centers, but its pharmacological activity is believed due solely to the d-threo isomer. We have found that d,l-threo-C-11 MP has favorable properties for PET studies, and therefore examined the effects of incorporating halogen atoms into the phenyl ring of MP, with a view to preparing C-11 and I-123 MP analogs as potential PET/SPECT tracers. We synthesized the 2-, 3- and 4-bromo MP analogs from the corresponding bromophenylacetonitriles by modification of the original synthesis of MP. In in vitro binding assays all three d,l-threo bromo compounds had higher affinities than MP for DA transporter sites labeled with tritiated WIN 35,428 (3->4-, 2->MP). They also showed high activity with NE reuptake sites labeled with tritiated nisoxetine. They were active in vivo as demonstrated by inhibition of heart uptake of tritiated NE in the mouse, and elevation of striatal extracellular DA (microdialysis) and stimulation of locomotor activity in the rat.

Pan, D.; Gatley, S.J.; Dewey, S.L. [Brookhaven National Lab., Upton, NY (United States)] [and others



Determinação do extremo de baixa luminosidade da função de luminosidade através de simulações de Monte Carlo  

NASA Astrophysics Data System (ADS)

Neste trabalho apresentamos os principais resultados do estudo da população de galáxias anãs de baixo brilho superficial dos grupos compactos HCG44 e HCG68. A inclinação do extremo de baixa luminosidade a da função de luminosidade desses grupos foi estimado através de simulações de Monte Carlo. O método consistiu em simular galáxias anãs de baixo brilho superficial em imagens reais seguindo uma distribuição de magnitudes dada uma função de Schechter. Foram testados vários modelos com diferentes valores para os parâmetros M* e a da função de Schechter, e para cada um dos modelos foi repetido o mesmo processo de recuperação, seleção e análise usados com as galáxias anãs dos grupos. Os resultados de cada modelo foram então comparados com o observado nos grupos. Nossos resultados indicam que a distribuição de magnitudes (para magnitudes absolutas até MR»-12) observadas nos grupos são compatíveis com uma função de luminosidade de Schechter dada por um M* = -16 e um a entre -1.0 e -1.4, e portanto de acordo com a tendência observada de uma inclinação do extremo de baixa luminosidade da função de luminosidade de grupos mais plana (~1.2, Trentham & Tully 2002, MNRAS, 335, 712) que esperado pelo modelo hierárquico de formação de estruturas CDM (Cold Dark Matter - Matéria Escura Fria).

Campos, P. E.; Mendes de Oliveira, C.



A Utilização da Astronomia como Tema Interdisciplinar e Aplicações de Objetos de Aprendizagem  

NASA Astrophysics Data System (ADS)

Este trabalho visa analisar a possibilidade de relacionar conteúdos aplicados no ensino fundamental e médio de forma interdisciplinar por intermédio da astronomia, com a intervenção de objetos de aprendizagem que possam integrar as disciplinas e a utilização de recursos tecnológicos. Em uma pesquisa prévia com 20 professores de uma escola estadual situada na cidade de Guarulhos foi observado que apenas 25% dos professores utilizam algum recurso tecnológico para o desenvolvimento de conteúdos pertinentes à sua disciplina, tais como sites e softwares educativos, sendo que a maioria absoluta continua ensinando apenas com livros didáticos. A maior parte dos professores apresenta dificuldades em trabalhar sua disciplina de forma interdisciplinar, ou seja, 75% dos professores preferem aplicar os conteúdos seguindo uma hierarquia linear de tópicos, evitando a discussão de temas que de alguma forma estão relacionados. A astronomia pode vir à fascinar o ser humano e despertar sua curiosidade promovendo um maior interesse no aprendizado, podendo favorecer análises interdisciplinares de forma lógica e objetiva, desta forma colocar a astronomia como tema motivador interdisciplinar, pode ser relevante no que se refere ao distanciamento da fragmentação dos conteúdos. No Estado de São Paulo, a implantação da proposta curricular no ensino fundamental e médio mostra claramente a inserção da astronomia na maior parte das séries, principalmente na 6ª série em que todo o bimestre se fala de astronomia.

da Silva, L. A.; Voelzke, M. R.



Determinação da massa de júpiter a partir das órbitas de seus satélites: um experimento didático  

NASA Astrophysics Data System (ADS)

Este trabalho apresenta o roteiro piloto de uma prática observacional em astronomia, junto com os primeiros resultados obtidos nesta fase de implementacão. O projeto, que será executado em duas etapas, visa introduzir noções de Astronomia a alunos do Ensino Médio e iniciantes nos cursos de Física. O experimento consiste em medir as órbitas dos satélites Galileanos e, a partir da análise dos dados coletados, verificar a validade da Lei das órbitas de Kepler, determinando a massa do planeta Júpiter. Em uma primeira etapa, as observações serão feitas utilizando um telescópio Meade LX200 10" e câmera CCD para obter uma seqüência de imagens do planeta, que possibilitará medir o movimento de seus satélites. A segunda etapa terá início a partir do funcionamento do telescópio em modo robótico, com a possibilidade de observações via internet por instituições de ensino. Para o desenvolvimento deste experimento foram inicialmente coletadas várias imagens de Júpiter obtidas com os instrumentos citados acima. Estas imagens serviram como base para confecção dos roteiros para a experiência no nível médio e superior. Os roteiros serão inicialmente apresentados em uma home-page. Nela também se buscará uma contextualização histórica da experiência bem como o estabelecimento de relações com professores e alunos, propostas metodológicas e a disponibilização dos programas computacionais necessários para a utilização "on-line" pelos usuários. O projeto conta com apoio da Fundação VITAE.

Schlickmann, M. S.; Saito, R. K.; Becker, D. A.; Rezende, M. F., Jr.; Cid Fernandes, R.



Kaonic Atoms/nuclei Measurements at DA?NE:. Siddharta and Amadeus  

NASA Astrophysics Data System (ADS)

The DA?NE electron-positron collider at the Laboratori Nazionali di Frascati dell'INFN has made available a unique quality negative kaons “beam”. Two experiments, SIDDHARTA and AMADEUS, will use this beam to perform unprecedented precision measurements on kaonic atoms and kaonic nuclei. The scientific cases, together with the experimental setups, are briefly presented in this paper.

Curceanu (Petrascu), C.; Zmeskal, J.


Kaonic atoms and nuclei search at the DA?NE accelerator  

NASA Astrophysics Data System (ADS)

The DA?NE electron-positron collider at the Laboratori Nazionali di Frascati dell'INFN has made available a unique quality low-energy negative kaons "beam". Two experiments, SIDDHARTA and AMADEUS, will use this kaon source to perform unprecedented precision measurements on kaonic atoms and kaonic nuclei. The scientific cases, together with the experimental setups, are briefly presented in this paper.

Curceanu (Petrascu), C.; Siddharta Collaboration; Amadeus Collaboration



Next Generation PanDA Pilot for ATLAS and Other Experiments  

NASA Astrophysics Data System (ADS)

The Production and Distributed Analysis system (PanDA) has been in use in the ATLAS Experiment since 2005. It uses a sophisticated pilot system to execute submitted jobs on the worker nodes. While originally designed for ATLAS, the PanDA Pilot has recently been refactored to facilitate use outside of ATLAS. Experiments are now handled as plug-ins such that a new PanDA Pilot user only has to implement a set of prototyped methods in the plug-in classes, and provide a script that configures and runs the experiment-specific payload. We will give an overview of the Next Generation PanDA Pilot system and will present major features and recent improvements including live user payload debugging, data access via the Federated XRootD system, stage-out to alternative storage elements, support for the new ATLAS DDM system (Rucio), and an improved integration with glExec, as well as a description of the experiment-specific plug-in classes. The performance of the pilot system in processing LHC data on the OSG, LCG and Nordugrid infrastructures used by ATLAS will also be presented. We will describe plans for future development on the time scale of the next few years.

Nilsson, P.; Barreiro Megino, F.; Caballero Bejar, J.; De, K.; Hover, J.; Love, P.; Maeno, T.; Medrano Llamas, R.; Walker, R.; Wenaus, T.; Atlas Collaboration



Molluscan mega-hemocyanin: an ancient oxygen carrier tuned by a ~550 kDa polypeptide  

Microsoft Academic Search

BACKGROUND: The allosteric respiratory protein hemocyanin occurs in gastropods as tubular di-, tri- and multimers of a 35 × 18 nm, ring-like decamer with a collar complex at one opening. The decamer comprises five subunit dimers. The subunit, a 400 kDa polypeptide, is a concatenation of eight paralogous functional units. Their exact topology within the quaternary structure has recently been

Bernhard Lieb; Wolfgang Gebauer; Christos Gatsogiannis; Frank Depoix; Nadja Hellmann; Myroslaw G Harasewych; Ellen E Strong; Jürgen Markl



As relações da Revista Estudos Feministas com os movimentos de mulheres  

Microsoft Academic Search

R R R R Resumo esumo esumo esumo esumo: Este artigo tem como objetivo refletir sobre o diálogo da REF, nesses últimos 15 anos, com os movimentos de mulheres no Brasil. Recupera os temas dos diversos dossiês que, em grande medida, focaram temáticas de extrema relevância e atualidade para a agenda política do feminismo. Resgata alguns desses dossiês como exemplos

Leila Linhares Barsted



Influência da irrigação e do genótipo na produção de castanha em cajueiro-anão-precoce  

Microsoft Academic Search

(2) Resumo - Avaliou-se a influência da irrigação e do genótipo na produção de castanha em cajueiro-anão- precoce (Anacardium occidentale L.) durante três anos. Foram estudados três clones (CP 09, CP 76 e CP 1001) e quatro regimes hídricos (testemunha sem irrigação e intervalos de irrigação de um, três e cinco dias). O delineamento experimental foi em blocos ao acaso,

Vitor Hugo de Oliveira; Levi de Moura Barros; Raimundo Nonato de Lima



[Release characteristics in vitro and pharmacokinetics of da chuanxiong fang multiunit drug delivery system in rats].  


The drug release characteristics ofDa Chuanxiong Fang multiunit drug delivery system (DCXFMDDS) in vivo and in vitro were evaluated. Ferulic acid (FA) and senkyunolide I (SI) were used as marker components, which were two of the effective components of Da Chuanxiong Fang. And their contents were determined by HPLC. Drug release characteristics in vitro of DCXFMDDS and Da Chuanxiong pills and pharmacokinetics characteristics of DCXFMDDS and Da Chuanxiong Fang active fraction (DCXFAF) in rats were compared. It was obvious that FA released from the DCXFMDDS in a sustained fashion but SI in a fast fashion both in vitro and in vivo. The releasing process and the releasing mechanism of FA and SI from DCXFMDDS were different, but the AUC value indicated that compared with DCXFAF the extent of absorption of FA and SI from DCXFMDDS was increased. Though from the same multiunit drug delivery system, FA an SI had different drug release characteristics both in vitro and in vivo, and that may be one of the reason why DCXFMDDS has the good properties such as rapid and long-lasting effect and high efficiency. PMID:22121790

Wei, Yuan-feng; Zhang, Ning; Lin, Xiao; Feng, Yi



Amplatzer Closure of Atrial Septal Defect and da Vinci Robot-Assisted Repair of Vascular Ring  

Microsoft Academic Search

Technology for minimally invasive approaches to congenital heart disease is a rapidly evolving field. This case report reviews a novel approach to combining two of the newer technologies available to treat a pediatric patient with an atrial septal defect (ASD) and a vascular ring. This report is the first to describe the use of the da Vinci surgical system to

R. G. Ohye; E. J. Devaney; J. Graziano; A. Ludomirsky



Automatic localization of the da Vinci surgical instrument tips in 3-D transrectal ultrasound.  


Robot-assisted laparoscopic radical prostatectomy (RALRP) using the da Vinci surgical system is the current state-of-the-art treatment option for clinically confined prostate cancer. Given the limited field of view of the surgical site in RALRP, several groups have proposed the integration of transrectal ultrasound (TRUS) imaging in the surgical workflow to assist with accurate resection of the prostate and the sparing of the neurovascular bundles (NVBs). We previously introduced a robotic TRUS manipulator and a method for automatically tracking da Vinci surgical instruments with the TRUS imaging plane, in order to facilitate the integration of intraoperative TRUS in RALRP. Rapid and automatic registration of the kinematic frames of the da Vinci surgical system and the robotic TRUS probe manipulator is a critical component of the instrument tracking system. In this paper, we propose a fully automatic registration technique based on automatic 3-D TRUS localization of robot instrument tips pressed against the air-tissue boundary anterior to the prostate. The detection approach uses a multiscale filtering technique to identify and localize surgical instrument tips in the TRUS volume, and could also be used to detect other surface fiducials in 3-D ultrasound. Experiments have been performed using a tissue phantom and two ex vivo tissue samples to show the feasibility of the proposed methods. Also, an initial in vivo evaluation of the system has been carried out on a live anaesthetized dog with a da Vinci Si surgical system and a target registration error (defined as the root mean square distance of corresponding points after registration) of 2.68 mm has been achieved. Results show this method's accuracy and consistency for automatic registration of TRUS images to the da Vinci surgical system. PMID:23674418

Mohareri, Omid; Ramezani, Mahdi; Adebar, Troy K; Abolmaesumi, Purang; Salcudean, Septimiu E



The cooperative self-assembly of 25 and 23kDa amelogenins.  


Self-assembly of the extracellular matrix protein amelogenin is believed to play an essential role in regulating the growth and organization of enamel crystals during enamel formation. The full-length amelogenin uniquely regulates the growth, shape, and arrangement of enamel crystals. Protein hydrolysis will ultimately facilitate a tissue with high mineral content. Protein processing is however highly specific suggesting a functional role of the cleaved amelogenins in enamel maturation. Here we hypothesize that the cooperative self-assembly of the recombinant full-length amelogenin 25kDa and the 23kDa proteolytic cleavage product is a function of pH, mixing ratio and incubation time and is associated with the isoelectric point of the protein. Self-assembly of amelogenin into nanospheres which increased in size with increasing pH was observed by atomic force microscopy. Elongated structures of about 100nm length and 25nm width formed over several days for amelogenin 25 and 23kDa predominantly at pH-values of 6.5 and 7.5, respectively. When both proteins 25 and 23kDa were mixed, self-assembled nanostrings of 200-300nm length consisting of fused nanospheres were obtained at pH around 7.0 within 24h. The protein nanostrings formed links over time and a continuous mesh was obtained after 7 days. Electrical conductivity data also showed gradual changes when both amelogenins were mixed in solutions supporting the idea that elongated structures form over extended periods of time. We propose that due to the difference in the isoelectric point, self-assembled nanospheres composed of 23 or 25kDa amelogenin have opposite ionic charges at pH-values around 7.0 and thus experience ionic attraction that enables cooperative self-assembly. PMID:18845261

He, Xiaodong; Li, Wu; Habelitz, Stefan



The cooperative self-assembly of 25 and 23 kDa amelogenins  

PubMed Central

Self-assembly of the extracellular matrix protein amelogenin is believed to play an essential role in regulating the growth and organization of enamel crystals during enamel formation. The full-length amelogenin uniquely regulates the growth, shape, and arrangement of enamel crystals. Protein hydrolysis will ultimately facilitate a tissue with high mineral content. Protein processing is however highly specific suggesting a functional role of the cleaved amelogenins in enamel maturation. Here we hypothesize that the cooperative self-assembly of the recombinant full-length amelogenin 25 kDa and the 23 kDa proteolytic cleavage product is a function of pH, mixing ratio and incubation time and is associated with the isoelectric point of the protein. Self-assembly of amelogenin into nanospheres which increased in size with increasing pH was observed by atomic force microscopy. Elongated structures of about 100nm length and 25 nm width formed over several days for amelogenin 25 and 23 kDa predominantly at pH-values of 6.5 and 7.5, respectively. When both proteins 25 and 23 kDa were mixed, self-assembled nanostrings of 200–300 nm length consisting of fused nanospheres were obtained at pH around 7.0 within 24 h. The protein nanostrings formed links over time and a continuous mesh was obtained after 7 days. Electrical conductivity data also showed gradual changes when both amelogenins were mixed in solutions supporting the idea that elongated structures form over extended periods of time. We propose that due to the difference in the isoelectric point, self-assembled nanospheres composed of 23 or 25 kDa amelogenin have opposite ionic charges at pH-values around 7.0 and thus experience ionic attraction that enables cooperative self-assembly.

He, Xiaodong; Li, Wu; Habelitz, Stefan



Comportamento da dose glandular versus contraste do objeto em mamografia: determinação de formalismo semi-empírico para diferentes combinações alvo-filtro  

Microsoft Academic Search

OBJETIVO: Verificar o efeito da mudança no contraste do objeto, tempo de exposição e dose de radiação quando diferentes espessuras de filtração de molibdênio (Mo) e ródio (Rh) são empregadas em mamógrafos. MATERIAIS E MÉTODOS: Realizaram-se medidas da exposição na entrada da pele com uma câmara de ionização para diferentes espessuras para os filtros de Mo e Rh. Para determinar

Gabriela Hoff; Carlos Eduardo de Almeida; Gary T. Barnes



11 CFR 111.12 - Subpoenas and subpoenas duces tecum; depositions (2 U.S.C. 437d(a) (3), (4)).  

Code of Federal Regulations, 2011 CFR

...subpoenas duces tecum; depositions (2 U.S.C. 437d(a) (3), (4)). 111.12...GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111...subpoenas duces tecum; depositions (2 U.S.C. 437d(a) (3), (4))....



11 CFR 111.13 - Service of subpoenas, orders and notifications (2 U.S.C. 437d(a) (3), (4)).  

Code of Federal Regulations, 2011 CFR

...subpoenas, orders and notifications (2 U.S.C. 437d(a) (3), (4)). 111.13...GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § 111...subpoenas, orders and notifications (2 U.S.C. 437d(a) (3), (4))....



11 CFR 111.15 - Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)).  

Code of Federal Regulations, 2011 CFR

...Motions to quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4)). 111.15...GENERAL COMPLIANCE PROCEDURE (2 U.S.C. 437g, 437d(a)) Enforcement § quash or modify a subpoena (2 U.S.C. 437d(a) (3), (4))....




Microsoft Academic Search

L'approccio comunemente utilizzato per il calcolo teorico del campo di deformazione indotto da una sorgente sismica in un mezzo elastico è basato sull'equivalenza fra una dislocazione di taglio elementare e una doppia coppia di forze puntiformi. Il campo prodotto da una sorgente di dimensioni finite viene poi ottenuto tramite sovrapposizione lineare dei campi generati da una distribuzione di doppie coppie

S. Tinti; A. Armigliato; A. Manucci



Microsoft Academic Search

RESUMO: Este artigo propõe uma análise da imagem da mulher que se esboça em alguns artigos do Código Penal Brasileiro. Para isso, vale mo-nos de algumas orientações teóricas da Análise do Discurso, no que diz respeito a Formaçõe s Discursivas, Ação e Racionalidade e Intencionalidade. Através desse aparato teórico, o trabalho levanta algumas questões de ordem lingüístico-discursivas, fundadoras de um

Carla Roselma; Athayde GUIMARAES; Gustavo Miranda SILVA; Priscilla Chantal; Duarte MARCHESANI


In vitro expression of the 50-kDa and 30-kDa fragments of the P97 adhesin of Mycoplasma hyopneumoniae in Escherichia coli and their use for serodiagnosis  

PubMed Central

This article reports the cloning and expression of 2 fragments of the P97 adhesin of Mycoplasma hyopneumoniae for use in serodiagnosis: a 50-kDa fragment (including the N-terminal cleavage site) and a 30-kDa fragment (including the C-terminal R1 and R2 repeats, which are essential for adherence). The genes encoding the fragments were amplified, cloned, and expressed in the Escherichia coli expression system BL21(DE3)pLysS. Antiserum against the purified recombinant proteins reacted with the mycoplasmal 97-kDa intact protein and the 66-kDa major cleavage fragment, confirming that both cloned fragments could induce antigen-specific antibodies in mice. Of 70 serum samples from nonvaccinated pigs, 26 (37%) were seropositive when the 30-kDa fragment was used as an antigen for enzyme-linked immunosorbent assay, suggesting that natural mycoplasmal infection is quite common in Korea. However, only 4 samples were seropositive when the 50-kDa fragment was used; this fragment was therefore deemed unsuitable for serodiagnosis. The 30-kDa fragment protein might be useful for measuring antibody response to vaccination and for detecting mycoplasmal infection.

Jang, Eun Jin; Kim, Tae Jung



87-kDa protein, a major specific substrate for protein kinase C: purification from bovine brain and characterization  

SciTech Connect

The 87-kDa protein, a major specific substrate for protein kinase C, has been purified 500-fold to apparent homogeneity from bovine forebrain supernatant. The purification procedure included batch adsorption to DE-52 (DEAE-cellulose), (NH/sub 4/)/sub 2/SO/sub 4/ precipitation, and chromatography on DEAE-Sephacel, Bio-Gel HTP (hydroxylapatite), Sephacryl S-400, and fast protein liquid chromatography ProRPC. The amino acid composition was notable for its high proportion of alanine (28.6 mol%) and its enrichment in glutamate/glutamine (18.1 mol%), glycine (12.6 mol%), and proline (11.3 mol%). The partial specific volume was 0.702 ml/g; the Stokes radius and sedimentation coefficient were 85 A and 2.11 S, respectively. Although the relative molecular mass of the protein on NaDodSO/sub 4//8% PAGE was 87-90 kDa, the molecular mass as determined from the above values was 68 kDa. The frictional ratio was 3.2, and the axial ratio was 60, indicating that the 87-kDa protein is an extremely elongated monomer. The purified 87-kDa protein was phosphorylated by purified protein kinase C to a stoichiometry of 2.2 mol of /sup 32/P per mol of 87-kDa protein (calculated using a value of 68 kDa for the molecular mass). Phosphorylation was exclusively on serine residues.

Albert, K.A.; Nairn, A.C.; Greengard, P.



Whole-genome sequences of DA and F344 rats with different susceptibilities to arthritis, autoimmunity, inflammation and cancer.  


DA (D-blood group of Palm and Agouti, also known as Dark Agouti) and F344 (Fischer) are two inbred rat strains with differences in several phenotypes, including susceptibility to autoimmune disease models and inflammatory responses. While these strains have been extensively studied, little information is available about the DA and F344 genomes, as only the Brown Norway (BN) and spontaneously hypertensive rat strains have been sequenced to date. Here we report the sequencing of the DA and F344 genomes using next-generation Illumina paired-end read technology and the first de novo assembly of a rat genome. DA and F344 were sequenced with an average depth of 32-fold, covered 98.9% of the BN reference genome, and included 97.97% of known rat ESTs. New sequences could be assigned to 59 million positions with previously unknown data in the BN reference genome. Differences between DA, F344, and BN included 19 million positions in novel scaffolds, 4.09 million single nucleotide polymorphisms (SNPs) (including 1.37 million new SNPs), 458,224 short insertions and deletions, and 58,174 structural variants. Genetic differences between DA, F344, and BN, including high-impact SNPs and short insertions and deletions affecting >2500 genes, are likely to account for most of the phenotypic variation between these strains. The new DA and F344 genome sequencing data should facilitate gene discovery efforts in rat models of human disease. PMID:23695301

Guo, Xiaosen; Brenner, Max; Zhang, Xuemei; Laragione, Teresina; Tai, Shuaishuai; Li, Yanhong; Bu, Junjie; Yin, Ye; Shah, Anish A; Kwan, Kevin; Li, Yingrui; Jun, Wang; Gulko, Pércio S



Immunological screening and characterization of highly specific monoclonal antibodies against 20 kDa hGH.  


Background: hGH has been widely abused as a doping agent in sports for many years. There are some important approaches for the detection of hGH doping, and the ratio of 22:20 kDa GH was considered one of the most suitable detection indicators of GH abuse. Currently, effective anti-GH antibodies and related reagents are needed to develop a detection method, in particular, highly specific anti-20 kDa hGH monoclonal antibodies are a prerequisite. Herein we constructed the expression vector of 20 kDa hGH and prepared the corresponding antibodies by the immunization of the recombinant human 20 kDa into mice. Positive clones that can specifically recognize 20 kDa hGH were screened and characterized by enzyme immunoassay, Dot-ELISA and surface plasmon resonance. In total, 14 specific monoclonal cell lines were screened out. Results: By a series of characterization, it was found that the 6C8, 44H3, 12G7 and 33Y19 clones were showing much higher specificity and affinity to 20 kDa hGH, and P3H9 could recognize both 20 and 22 kDa hGH isoforms. 6C8 and 44H3 matched well with P3H9 in the surface plasmon resonance testing. The 12G7 clone had the best surface properties with an association constant of 3.4 × 10(9) M(-1) and a dissociation constant of 2.95 × 10(10) M. Conclusion: Highly specific monoclonal antibodies against 20 kDa hGH were generated, and also two paired antibodies (P3H9 and 6C8 or P3H9 and 44H3) were characterized, which can serve as the potential components for 22:20 kDa detection kit. PMID:23013398

Du, Hongwu; Chen, Guangyu; Wang, Shuang; Wang, Shan; Li, Shaoxu; Li, Chuanbao; Xun, Yiping; He, Chunji; Wu, Moutian



16-kDa Prolactin Reduces Angiogenesis, but Not Growth of Human Breast Cancer Tumors In Vivo  

Microsoft Academic Search

Prolactin (PRL) is a peptide hormone necessary for normal growth and development of the human breast. In addition, high levels\\u000a of PRL in plasma correlate with increased risk of breast cancer, especially among postmenopausal women. Several isoforms of\\u000a PRL exist in human circulation, including a 16-kDa isoform that is an N-terminal fragment of the full-length 23-kDa PRL. 16-kDa\\u000a PRL has

Jessica M. Faupel-Badger; Erika Ginsburg; Jodie M. Fleming; Leah Susser; Teresa Doucet; Barbara K. Vonderhaar



O Imperio como Argumento: Um Contraponto entre Joaquim Nabuco e o Bispo D. Jose Mauricio da Rocha (The Empire as Argument: A Counterpoint between Joaquim Nabuco and the Bishop Dom Jose Mauricio da Rocha).  

ERIC Educational Resources Information Center

Dialogs with the previous article establishing a comparative analysis between the political acceptance present in the reactionary speech of Dom Jose Mauricio da Rocha and in the monarchist words of Joaquim Nabuco. (PA)

Marson, Izabel Andrade



Seguranca radiologica no uso de tecnicas nucleares -atividades desenvolvidas pelo Centro de Desenvolvimento da Tecnologia Nuclear - CNEN. (Radiological safety in the use of nuclear technique-activities developed by Centro de Desenvolvimento da Tecnologia Nuclear - CNEN).  

National Technical Information Service (NTIS)

The activities developed by the Centro de Desenvolvimento da Tecnologia Nuclear - CDTN supporting the users of nuclear techniques are described. The methodology used and future plans for the group are shown. (E.G.). (Atomindex citation 22:055424)

T. Wakabayashi E. G. Pereira T. Hirayama



Monodisperse 130 kDa and 260 kDa Recombinant Human Hemoglobin Polymers as Scaffolds for Protein Engineering of Hemoglobin-Based Oxygen Carriers  

PubMed Central

A recombinant 130 kDa dihemoglobin which is made up of a single-chain tetra-? globin and four ? globins has been expressed as a soluble protein in E. coli. The sequence of the single chain tetra-? is: ?I-Gly-?II-(SerGlyGly)5Ser-?III-Gly-?IV. This dihemoglobin has been purified and characterized in vitro by size exclusion chromatography, electrospray mass spectroscopy, equilibrium oxygen binding, and analytical ultracentrifugation. The observed values of P50 and nmax for the dihemoglobin are slightly lower than those observed for the recombinant hemoglobin rHb1.1 (a “monohemoglobin” comprised of two ? globins and an ?I-Gly-?II di?-globin chain). Titration of the deoxy form of dihemoglobin with CO shows that all eight heme centers bind ligand. In vivo, dihemoglobin showed increased circulating halflife and a reduced pressor response in conscious rats when compared to rHb1.1. These observations suggest that dihemoglobin is an oxygen carrying molecule with desirable in vivo properties and provides a platform for an isooncotic hemoglobin solution derived solely from a recombinant source. A 260 kDa tetrahemoglobin has also been produced by chemical crosslinking of a dihemoglobin that contains a Lys16Cys mutation in the C-terminal ?-globin subunit. Tetrahemoglobin also shows reduced vasoactivity in conscious rats that is comparable to that observed for dihemoglobin.

Marquardt, David A.; Doyle, Michael P.; Davidson, Jeffrey S.; Epp, Janet K.; Aitken, Jacqueline F.; Lemon, Douglas D.; Anthony-Cahill, Spencer J.



The same nuclear proteins bind to the 5?-flanking regions of genes for the rice seed storage protein: 16 kDa albumin, 13 kDa prolamin and type II glutelin  

Microsoft Academic Search

Expression of rice seed storage-protein genes is dramatically regulated over a short period of seed maturation. To characterize the expression mechanism of the rice seed storage protein genes, their expression of major storage protein genes (16 kDa albumin, 13 kDa prolamin and type II glutelin) were compared by RNA blot analysis. Their coordinate expression suggests that the transcriptional regulatory machinery

Masayuki Nakase; Takehisa Yamada; Takahiro Kira; Junji Yamaguchi; Naohito Aoki; Ryo Nakamura; Tsukasa Matsuda; Takahiro Adachi



Iron abundance in the hot DA white dwarfs Feige 24 and G191 B2B  

NASA Technical Reports Server (NTRS)

Attention is given to model calculations of the far- and extreme-UV line spectra of highly ionized Fe species (Fe IV, Fe V, and Fe VI) for hot high-gravity H-rich stars. A spectral analysis of 31 hr of exposure of the DA white dwarf Feige 24 with IUE in the echelle mode reveals the presence of Fe with an abundance relative to H by number of (5-10) x 10 exp -6 with an uncertainty dominated by the determination of stellar parameters. An analysis of IUE data from the white dwarf G191 B2B results in a similar Fe abundance if this star shares similar atmospheric parameters (Teff, g) with Feige 24. Fe is thus the second most abundant photospheric element in hot DA white dwarfs.

Vennes, Stephane; Chayer, Pierre; Thorstensen, John R.; Bowyer, Stuart; Shipman, Harry L.



Visual degradation in Leonardo da Vinci's iconic self-portrait: A nanoscale study  

NASA Astrophysics Data System (ADS)

The discoloration of ancient paper, due to the development of oxidized groups acting as chromophores in its chief component, cellulose, is responsible for severe visual degradation in ancient artifacts. By adopting a non-destructive approach based on the combination of optical reflectance measurements and time-dependent density functional theory ab-initio calculations, we describe and quantify the chromophores affecting Leonardo da Vinci's iconic self-portrait. Their relative concentrations are very similar to those measured in modern and ancient samples aged in humid environments. This analysis quantifies the present level of optical degradation of the Leonardo da Vinci's self-portrait which, compared with future measurements, will assess its degradation rate. This is a fundamental information in order to plan appropriate conservation strategies.

Conte, A. Mosca; Pulci, O.; Misiti, M. C.; Lojewska, J.; Teodonio, L.; Violante, C.; Missori, M.



Antigenic secreted proteins from Haemophilus paragallinarum. A 110-kDa putative RTX protein.  


Haemophilus paragallinarum is the causal agent of infectious coryza, an economically important disease for the poultry industry. This bacterium secreted proteins of 25-110 kDa during its growth in brain heart infusion, tryptic soy broth, or Luria-Bertani glucose phosphate media, all lacking serum. Some of these proteins were recognized by sera from chickens experimentally infected with H. paragallinarum. A 110-kDa protein was recognized by a serum pool from convalescent-phase pigs naturally infected with Actinobacillus pleuropneumoniae, and also by a rabbit polyclonal serum against Apx I as well as a rabbit serum against Mannheimia haemolytica leukotoxin, suggesting the presence of an RTX-like protein in H. paragallinarum. H. paragallinarum secreted proteins could be important immunogens in the control of infectious coryza. PMID:15019738

Mena-Rojas, Erika; Vázquez Cruz, Candelario; Vaca Pacheco, Sergio; García González, Octavio; Pérez-Márquez, Víctor M; Pérez-Méndez, Alma; Ibarra-Caballero, Jorge; de la Garza, Mireya; Zenteno, Edgar; Negrete-Abascal, Erasmo



Optimizing IrDA throughput by including processing time with physical layer consideration  

NASA Astrophysics Data System (ADS)

Feature Issue on Optical Wireless Communications (OWC) We derive new equations linking the Infrared Data Association (IrDA) data link layer (IrLAP) throughput to the physical layer by providing the bit error rate in relation to link distance and various physical layer parameters. Furthermore, we optimize the link by simultaneously adapting the frame and window size. The optimum equations include the effect of processor speed. The mathematical analysis is validated by comparison with exact numerical methods. Analytical results presented in the paper explore the effect of frame processing time on improving the performance of IrDA links and identify the improvement in throughput performance by utilizing the derived optimum values.

Huang, Pi; Chatzimisios, P.; Boucouvalas, A. C.



Isolation and characterization of a 22 kDa protein with antifungal properties from maize seeds.  


We have purified a 22 kDa protein from maize seeds to homogeneity by ammonium sulfate precipitation, chitin extraction and Mono-S column chromatography. The purified protein inhibited the growth of the agronomically important pathogens of potato wilt (Fusarium oxysporum) and tomato early blight (Alternaria solani). Sequence analysis of the purified protein showed that it has 52% homology with the sweet protein thaumatin (Edens, L., Hselinga, L., Klok, R., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. (1982) Gene 18, 1-12), 57% homology with the pathogenesis-related protein (Cornelissen, B. J. C., Huijsduijnen, R. A. M., and Bol, J. F. (1986) Nature 321, 531-532) and 99% homology with the 22 kDa trypsin/alpha-amylase inhibitor (Richardson, M., Valdes-Rodriguez, S., and Blanco-Labra, A. (1987) Nature 327, 432-434). PMID:1731773

Huynh, Q K; Borgmeyer, J R; Zobel, J F



Development of noSQL data storage for the ATLAS PanDA Monitoring System  

NASA Astrophysics Data System (ADS)

For several years the PanDA Workload Management System has been the basis for distributed production and analysis for the ATLAS experiment at the LHC. Since the start of data taking PanDA usage has ramped up steadily, typically exceeding 500k completed jobs/day by June 2011. The associated monitoring data volume has been rising as well, to levels that present a new set of challenges in the areas of database scalability and monitoring system performance and efficiency. These challenges are being met with a R&D effort aimed at implementing a scalable and efficient monitoring data storage based on a noSQL solution (Cassandra). We present our motivations for using this technology, as well as data design and the techniques used for efficient indexing of the data. We also discuss the hardware requirements as they were determined by testing with actual data and realistic loads.

Potekhin, M.; ATLAS Collaboration



Analysis of DA White Dwarfs from the McCook & Sion Catalog  

NASA Astrophysics Data System (ADS)

The Sloan Digital Sky Survey has already doubled the number of spectroscopically identified white dwarfs. However, there remains a wealth of white dwarfs in the McCook & Sion catalog for which little or nothing is known. We have thus undertaken a spectroscopic survey of all Northern hemisphere DA white dwarfs from this catalog in order to (1) confirm the spectroscopic classification, and (2) provide measurements of Teff and log g for each star. As part of this project, we have also secured spectroscopic data for all DA stars with high-speed photometric measurements available, in order to study the purity of the ZZ Ceti instability strip, which has recently been challenged, once again.

Gianninas, A.; Bergeron, P.; Dufour, P.



Initial insight into the function of the lysosomal 66.3 kDa protein from mouse by means of X-ray crystallography  

PubMed Central

Background The lysosomal 66.3 kDa protein from mouse is a soluble, mannose 6-phosphate containing protein of so far unknown function. It is synthesized as a glycosylated 75 kDa precursor that undergoes limited proteolysis leading to a 28 kDa N- and a 40 kDa C-terminal fragment. Results In order to gain insight into the function and the post-translational maturation process of the glycosylated 66.3 kDa protein, three crystal structures were determined that represent different maturation states. These structures demonstrate that the 28 kDa and 40 kDa fragment which have been derived by a proteolytic cleavage remain associated. Mass spectrometric analysis confirmed the subsequent trimming of the C-terminus of the 28 kDa fragment making a large pocket accessible, at the bottom of which the putative active site is located. The crystal structures reveal a significant similarity of the 66.3 kDa protein to several bacterial hydrolases. The core ???? sandwich fold and a cysteine residue at the N-terminus of the 40 kDa fragment (C249) classify the 66.3 kDa protein as a member of the structurally defined N-terminal nucleophile (Ntn) hydrolase superfamily. Conclusion Due to the close resemblance of the 66.3 kDa protein to members of the Ntn hydrolase superfamily a hydrolytic activity on substrates containing a non-peptide amide bond seems reasonable. The structural homology which comprises both the overall fold and essential active site residues also implies an autocatalytic maturation process of the lysosomal 66.3 kDa protein. Upon the proteolytic cleavage between S248 and C249, a deep pocket becomes solvent accessible, which harbors the putative active site of the 66.3 kDa protein.

Lakomek, Kristina; Dickmanns, Achim; Kettwig, Matthias; Urlaub, Henning; Ficner, Ralf; Lubke, Torben



A constitutively expressed 36 kDa exochitinase from Bacillus thuringiensis HD1  

Microsoft Academic Search

A 36kDa chitinase was purified by ion exchange and gel filtration chromatography from the culture supernatant of Bacillus thuringiensis HD-1. The chitinase production was independent of the presence of chitin in the growth medium and was produced even in the presence of glucose. The purified chitinase was active at acidic pH, had an optimal activity at pH 6.5, and showed

Naresh Arora; Tarannum Ahmad; R Rajagopal; Raj K Bhatnagar



27 kDa Fasciola gigantica Glycoprotein for the Diagnosis of Prepatent Fasciolosis in Cattle  

Microsoft Academic Search

An indirect enzyme-linked immunosorbent assay (ELISA) using 27 kDa glycoprotein of Fasciola gigantica has been evaluated for its potential use in the diagnosis of bovine fasciolosis. Following experimental infection of rabbits, F. gigantica infection-induced antibodies were isolated and later used as ligands in affinity chromatography for isolation of infection-induced antibody-specific proteins. Among the five infection-specific proteins isolated, a glycoprotein of

S. Ghosh; P. Rawat; R. Velusamy; D. Joseph; S. C. Gupta; B. P. Singh



Separation of glucose oxidase and catalase using ultrafiltration with 300-kDa polyethersulfone membranes  

Microsoft Academic Search

This work examines the separation of glucose oxidase (GOx) and catalase by ultrafiltration using 300kDa MWCO polyethersulfone membranes. The effects of solution pH and ionic strength on the transmission of a single protein (either GOx or catalase) were quantified using the pulsed sample injection technique. Other effects including stirring speed and permeate flux on separation of the binary protein mixtures

Jianguo Liu; Junren Lu; Xiubo Zhao; Jianren Lu; Zhanfeng Cui



A 200 kDa protein is associated with haemagglutinating isolates of Moraxella (Branhamella) catarrhalis  

Microsoft Academic Search

Moraxella catarrhalis adheres to human erythrocytes by means of a proteinaceous, trypsin sensitive, heat modifiable haemagglutinin. A 200 kDa protein was found to be associated with haemagglutinating isolates of M. catarrhalis. This protein was present on all haemagglutinating isolates (n=17), but was absent on the non-haemagglutinating isolates (n=23) examined. This protein demonstrated heat-modifiable properties in sodium dodecyl sulfate and was

Margaret Fitzgerald; Riona Mulcahy; Susan Murphy; Conor Keane; Davis Coakley; Thomas Scott



Peculiarity of Hexamethylenetetratellurafulvalene (HMTTeF) Charge Transfer Complexes of Donor-Acceptor (DA) Type  

Microsoft Academic Search

The complex formation of hexamethylenetetratellurafulvalene (HMTTeF) with 28 kinds of organic electron acceptors yielded 31 charge transfer (CT) complexes. The infrared and ultra-violet–visible–near-infrared spectra of the complexes were examined to study the ionicity of their ground states in solid. A plot of CT transition energies and the difference of redox potentials; ?E(DA) of donor (D) and acceptor (A) molecules indicated

Sang-Soo Pac; Gunzi Saito



Involvement of the 90 kDa glycoprotein in adhesion of Nectria haematococca macroconidia  

Microsoft Academic Search

Previously we demonstrated thatNectria haematococcamacroconidia become adhesive within 5 min and adhere at the spore apices to substrata and to other spores. At the same time, the macroconidia produce mucilage at the spore tips which binds Concanavalin A (Con A), and produce a macroconidial-specific 90 kDa glycoprotein which binds Con A; Con A inhibits macroconidial adhesion. Here we demonstrate that

Y. H Kwon; L Epstein



Purification and properties of an oestrogen-stimulated mouse uterine glycoprotein (approx. 70 kDa).  

PubMed Central

An oestrogen-induced secretory protein from mouse uterine luminal fluid was purified by CM-Affi-Gel Blue chromatography and reverse-phase h.p.l.c. This protein has an apparent molecular mass of approx. 70 kDa both by SDS/polyacrylamide-gel electrophoresis (with or without 2-mercaptoethanol) and by gel-filtration column chromatography, indicating that it exists as a single-chain polypeptide. Further analysis of the protein revealed that it is highly basic (pI greater than or equal to 10) and is a glycoprotein. The N-terminus appears to be blocked to Edman degradation. The partial amino acid sequence of a fragment was obtained by cleavage with CNBr; no sequence homology was apparent between the analysed fragment and other known sequences. The incorporation of [35S]methionine into uterine proteins in vitro revealed that oestrogen treatment of immature mice stimulates both synthesis and secretion of the 70 kDa protein. An enzyme-linked immunosorbent assay with polyclonal antibody was used to determine the tissue distribution of the protein. Tissues such as lung, brain, spleen, muscle, intestine, liver, kidney and ovary of oestrogen-treated mice did not have detectable amounts of the 70 kDa protein. Immunoreactivity was present in uterine and vaginal tissues from oestrogen-treated animals. The 70 kDa protein was not induced by testosterone or progesterone. Although the function of this protein is unknown, it is useful as a marker for the study of oestrogen action in the mammalian uterus as well as regulation of gene expression at the molecular level. Images Fig. 1. Fig. 2. Fig. 3. Fig. 4. Fig. 6.

Teng, C T; Walker, M P; Bhattacharyya, S N; Klapper, D G; DiAugustine, R P; McLachlan, J A



Localization of 70-kDa stress protein induction in gerbil brain after ischemia  

Microsoft Academic Search

Induction of the 70-kDa heat shock protein, hsp70, has been demonstrated in brain following experimental stroke. In the present study, hsp70 was localized in gerbil brain at intervals after transient ischemia using a monoclonal antibody specific for stress-inducible forms of hsp70-related proteins. Induced immunoreactivity was found only in neurons, primarily in hippocampus, striatum, entorhinal cortex and some neocortical regions. Notably

K. Vass; W. J. Welch; T. S. Nowak



Protein 70 kDa in the control of sleep and thermoregulation  

Microsoft Academic Search

Studies of expression of molecular chaperones of the family of Heat Shock Proteins 70 kDa (HSP70) in the mouse and rat brain\\u000a during sleep deprivation do not answer the question whether the HSP70 produce somnogenic effect. In the present work there\\u000a are studied effects of exogenous Hsp70 that is known to be able to penetrate into living cells in vitro

Yu. F. Pastukhov; I. V. Ekimova; K. A. Khudik; I. V. Guzhova



Geological Structure of the Quaternary Sedimentary Sequence in the Klaip?da Strait, Southeastern Baltic  

Microsoft Academic Search

\\u000a The Klaip?da Strait is located between the Curonian Spit and the mainland coast of Lithuania. It links the Curonian Lagoon\\u000a with the Baltic Sea. The Quaternary sequence is represented here by Pleistocene sediments formed during a few glaciations\\u000a and interglacials. Its uppermost part is composed of Late glacial and Holocene sediments originating from different stages\\u000a of the Baltic Sea development.

Albertas Bitinas; Aldona Damušyt?; Anatoly Molodkov


Identification and localization of a 94 kDa membrane protein found in Mycoplasma bovoculi strains  

Microsoft Academic Search

Six isolates of Mycoplasma bovoculi obtained from cattle herds with bovine keratoconjunctivitis were analyzed by gel electrophoresis and immunoblotting techniques. All six strains showed similarity in their protein profiles although no two patterns were identical. Antigenic differences between strains were detected in immunoblots reacted with post-exposure calf serum. A common 94 kDa protein band designated p94 was detected in all

Barik A Salih; Ricardo F Rosenbusch



Microscopic Observations on the Origin of Defects During Machining of Direct Aged (DA) Inconel 718 Superalloy  

Microsoft Academic Search

Surface quality of advanced superalloys after machining is one of the major issues in the aerospace industry because it directly\\u000a affects service characteristics of the machined part. Tool life of cemented carbide inserts with the TiAlN coating during\\u000a machining of direct aged DA 718 alloys under roughing and finishing conditions has been under study. The defect origin on\\u000a the surface

G. K. Dosbaeva; S. C. Veldhuis; A. Elfizy; G. Fox-Rabinovich; T. Wagg



Comparação da sensibilidade de bovinos e búfalos à intoxicação por Palicourea marcgravii (Rubiaceae)1  

Microsoft Academic Search

Barbosa J. D., Oliveira C. M. C., Tokarnia C. H. & Riet-Correa F. 2003. (Comparison of the sensibility of cattle and buffaloes to poisoning by Palicourea marcgravii (Rubiaceae).) Comparação da sensibilidade de bovinos e búfalos à intoxicação por Palicourea marcgravii (Rubiaceae). Pesquisa Veterinária Brasileira 23(4):167-172. Escola de Medicina Veterinária, Campus Castanhal, Universidade Federal do Pará, Rua Maximino Porpino 1000, Castanhal,

José Diomedes Barbosa; Carlos Magno; Chaves de Oliveira; Carlos Hubinger


Photochemical modification of 10 kDa polyethersulfone ultrafiltration membranes for reduction of biofouling  

Microsoft Academic Search

Poly(ether sulfone) 10kDa ultrafiltration membranes were modified by photolysis using ultraviolet light and graft polymerization of hydrophilic monomers onto the membrane surface to create more hydrophilic and lower fouling membrane surfaces. The modified membrane surfaces were characterized by FTIR\\/ATR and captive bubble contact angle measurements to determine chemical and hydrophilicity changes during modification. The modified membranes were compared with an

John Pieracci; James V Crivello; Georges Belfort



Concertação e efetividade da ação política: o Conselho de Desenvolvimento Econômico e Social do governo Lula  

Microsoft Academic Search

A experiência internacional de Conselhos de Desenvolvimento remonta à metade do século passado com a criação dos primeiros conselhos na França (1946\\/1958) e Holanda (1950), vinculando-se à tradição de negociação e representação corporativa que fundamentou a experiência da social-democracia européia. Atualmente também existem conselhos na Áustria (1963), em Portugal (1991\\/1992), na Espanha (1991), na África do Sul (1995), além do

Sonia Fleury



Una nueva especie de Pachynolophus (Mammalia, Perissodactyla) de Zambrana (Álava, Región Vasco-Cantábrica). Análisis filogenético de Pachynolophus y primera cita en el Eoceno superior de la Península Ibérica  

Microsoft Academic Search

Recent fieldwork in the Late Eocene of Zambrana (Álava, Basque-Cantabrian Region) has yielded a new species of Pachynolophus: P. zambranensis nov. sp. According to the phylogenetic analysis made in this study, the Zambrana species is part of the monophyletic Pachynolophus, which is defined at least by three synapomorphies: significant progressive increase of the size in the M1\\/1-M3\\/3 series, being M3\\/3

Ainara Badiola; Xabier Pereda-Suberbiola; Miguel Angel Cuesta



76 FR 56455 - DaVita, Inc.; Analysis of Agreement Containing Consent Orders To Aid Public Comment  

Federal Register 2010, 2011, 2012, 2013

...for confidential treatment, and you have to...for confidential treatment that accompanies...end stage renal disease (``ESRD'') patients receive treatment. In 2010 DaVita's...function of the kidneys by removing...



Model-Independent Determination of Curvature Parameter Using H(z) and DA (z) Data Pairs from BAO Measurements  

NASA Astrophysics Data System (ADS)

We present a model-independent determination of the curvature parameter ? k using the Hubble parameter H(z) and the angular diameter distance DA (z) from recent baryon acoustic oscillation (BAO) measurements. Each H(z) and DA (z) pair from a BAO measurement can constrain a curvature parameter. The accuracy of the curvature measurement improves with increased redshift of H(z) and DA (z) data. By using the H(z) and DA (z) pair derived from a BAO Lyman ? forest measurement at z = 2.36, the ? k is confined to be –0.05 ± 0.06, which is consistent with the curvature of -0.037^{+0.044}_{-0.042} constrained by the nine year Wilkinson Microwave Anisotropy Probe data only. Considering future BAO measurements, at least one order of magnitude improvement of this curvature measurement can be expected.

Li, Yun-Long; Li, Shi-Yu; Zhang, Tong-Jie; Li, Ti-Pei



Geologic and Geotechnical Contributions to the Lagoa DA Rabicha and Cachoeira Mines Projects, Lagoa Real, State of Bahia, Brazil.  

National Technical Information Service (NTIS)

The procedures and systematics involved in obtaining basic geologic-geotechnical, geomechanical and hydrogeotechnical parameters to serve as an aid in the planning of mining activities for the Lagoa da Rabicha and Cachoeira deposits, located in the Lagoa ...

A. A. Tognon J. Costanzo Junior L. M. Ojima T. Oliveira Braga M. Mueller



Diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT: a clinical follow up study  

PubMed Central

The [123I]ioflupane—a dopamine transporter radioligand—SPECT (DaT-SPECT) has proven to be useful in the differential diagnosis of tremor. Here, we investigate the diagnoses behind patients with hard-to-classify tremor and normal DaT-SPECT. Therefore, 30 patients with tremor and normal DaT-SPECT were followed up for 2 years. In 18 cases we were able to make a diagnosis. The residual 12 patients underwent a second DaT-SPECT, were then followed for additional 12 months and thereafter the diagnosis was reconsidered again. The final diagnoses included cases of essential tremor, dystonic tremor, multisystem atrophy, vascular parkinsonism, progressive supranuclear palsy, corticobasal degeneration, fragile X–associated tremor ataxia syndrome, psychogenic parkinsonism, iatrogenic parkinsonism and Parkinson's disease. However, for 6 patients the diagnosis remained uncertain. Larger series are needed to better establish the relative frequency of the different conditions behind these cases.

Menendez-Gonzalez, Manuel; Tavares, Francisco; Zeidan, Nahla; Salas-Pacheco, Jose M.; Arias-Carrion, Oscar



A área de proteção ambiental 'Fazenda e Parque do Carmo': os problemas ambientais, as dificuldades de gestão e a influência na qualidade de vida da população The environmental protection area \\  

Microsoft Academic Search

RESUMO: A falta de planejamento urbano da Cidade de São Paulo contribuiu para a eliminação da maior parte da cobertura vegetal, dada à ocupação irregular, inclusive de áreas legalmente protegidas. Essas áreas verdes são reguladoras do equilíbrio ambiental e assim, interferem diretamente na qualidade de vida da população. Através de levantamento bibliográfico e documental e entrevista com o Conselho Consultivo

Mirtes Moreira Silva; José Luís Negrão Mucci; Maria Cecília Focesi Pelicioni


A importância da poeira e ondas de Alfvén na estabilidade de nuvens moleculares anãs  

NASA Astrophysics Data System (ADS)

Nuvens moleculares anãs se apresentam dinamicamente estáveis, embora possuam massas muito maiores que a massa de Jeans. Por este motivo, a estabilidade destes objetos não pode ser explicada considerando-se apenas a pressão térmica. Campos magnéticos, aproximadamente uniformes e de ~mG, exercem um termo extra de pressão que sustenta a nuvem, mas somente na direção perpendicular às linhas de campo. Para a direção paralela, ondas de Alfvén geradas por turbulências no meio, por exemplo, têm sido utilizadas. Estas, sendo supostamente fracamente amortecidas, poderiam sustentar a nuvem nesta direção. Entretanto, estes meios contêm grandes quantidades de poeira carregada eletricamente. Estes grãos de poeira possuem frequências cíclotron, que podem entrar em ressonância com as ondas. Neste trabalho calculamos os efeitos que o amortecimento cíclotron da poeira teriam na propagação da onda, e consequentemente na estabilidade da nuvem. Considerando um fluxo de ondas, com um dado espectro de frequências, e uma população de grãos de poeira, com distribuição de tamanho observada, foi possível mostrar que o amortecimento é eficiente em uma larga banda de frequências. Neste caso as ondas seriam rapidamente amortecidas gerando pequenas condensações de alta densidade, e não poderiam ser utilizadas para explicar a estabilidade de uma nuvem inteira. Desta forma, rotação e turbulência seriam candidatos alternativos para garantir a estabilidade destes objetos.

Falceta-Gonçalves, D.; de Juli, M. C.; Jatenco-Pereira, V.



Microvariabilidade da polarização óptica e do fluxo infravermelho de 3C 279  

NASA Astrophysics Data System (ADS)

Neste trabalho mostramos um estudo sobre a existência e o comportamento da microvariabilidade na polarização óptica no blazar 3C 279. As medidas de polarização foram feitas durante quatro noites em março/2002 com o telescópio de 2.15 m do CASLEO, utilizando o polarímetro CASPROF. As observações mostraram que o grau de polarização linear é composto por duas componentes: uma relativa ao flickering em escalas de tempo de minutos e outra com variações em escalas de tempo de dias. Neste último caso a variação do grau de polarização está correlacionada com variações intraday no infravermelho próximo, cujos dados foram obtidos simultaneamente durante duas noites no telescópio de 1.60 m do LNA, utilizando a CamIV. Além disso, o ângulo de polarização apresentou uma variação súbita de mais de 10 graus em algumas horas, o que poderia ser causado pela injeção de um novo choque no jato. Esta idéia é apoiada pelo comportamento da variabilidade no infravermelho em escalas de tempo mais longas, que foi acompanhado durante três meses após o término da campanha coordenada com observações adicionais no LNA.

Andruchow, I.; Cellone, S. A.; Romero, G. E.; Dominici, T. P.; Abraham, Z.



Identification of a 24 kDa excretory secretory protein in Anisakis simplex.  


A gene coding for a 24 kDa protein (22 U homologous; As22U) was isolated from the Anisakis simplex third-stage larvae cDNA library during expressed sequence tag analysis. As22U was 636 bp long, and was found to code for 212 amino acid residues with a calculated mass of 23.5 kDa and a PI of 9.06. The As22U deduced amino acid sequence harbored a signal peptide region and 16 highly conserved cysteine residues, and it was identified in both the total extracts and excretory secretory (ES) protein of A. simplex. Its molecular weight was measured at 24 kDa via western blot analysis. The expression levels of thymic stromal lymphopoietin, IL-25, and CXCL1 (Gro-?) genes were increased at 6h after recombinant As22U treatment in mouse intestinal epithelial cells. Additionally, thymus and activation-regulated chemokine gene levels were increased at 14 h after treatment. Although we do not currently have sufficient evidence to determine whether As22U plays a role as an allergen, this remains possible. Further in vivo studies may provide some insight as to the allergenic properties of As22U. PMID:22001446

Park, Ji Seoung; Cho, Min Kyoung; Yu, Hak Sun; Ahn, Soon Cheol



Microscopic Observations on the Origin of Defects During Machining of Direct Aged (DA) Inconel 718 Superalloy  

NASA Astrophysics Data System (ADS)

Surface quality of advanced superalloys after machining is one of the major issues in the aerospace industry because it directly affects service characteristics of the machined part. Tool life of cemented carbide inserts with the TiAlN coating during machining of direct aged DA 718 alloys under roughing and finishing conditions has been under study. The defect origin on the surface of the machined part was investigated. Metallographic observations of the DA 718 were made using optical metallography and SEM/EDS. To find out the origins of surface defect formation, the morphology of machined parts and cross sections of the machined surfaces have been investigated. Two major categories of defects were detected on the surface of the machined part: cracks and tears. The origin of the cracks on the machined surface is related to shearing of the primary complex TiC/NbC carbide revealed in a structure of DA 718 alloy. At the same time, Nb-rich regions of the primary complex carbide interact with the environment (oxygen from air) during machining with further formation of low strength oxide layer on the surface, forming tears.

Dosbaeva, G. K.; Veldhuis, S. C.; Elfizy, A.; Fox-Rabinovich, G.; Wagg, T.



Surgical Outcomes of Congenital Atrial Septal Defect Using da VinciTM Surgical Robot System  

PubMed Central

Background Minimally invasive cardiac surgery has emerged as an alternative to conventional open surgery. This report reviews our experience with atrial septal defect using the da VinciTM surgical robot system. Materials and Methods This retrospective study included 50 consecutive patients who underwent atrial septal defect repair using the da VinciTM surgical robot system between October 2007 and May 2011. Among these, 13 patients (26%) were approached through a totally endoscopic approach and the others by mini-thoracotomy. Nineteen patients had concomitant procedures including tricuspid annuloplasty (n=10), mitral valvuloplasty (n=9), and maze procedure (n=4). The mean follow-up duration was 16.9±10.4 months. Results No remnant interatrial shunt was detected by intraoperative or postoperative echocardiography. The atrial septal defects were mainly repaired by Gore-Tex patch closure (80%). There was no operative mortality or serious surgical complications. The aortic cross clamping time and cardiopulmonary bypass time were 74.1±32.2 and 157.6±49.7 minutes, respectively. The postoperative hospital stay was 5.5±3.3 days. Conclusion The atrial septal defect repair with concomitant procedures like mitral valve repair or tricuspid valve repair using the da VinciTM system is a feasible method. In addition, in selected patients, complete port access can be helpful for better cosmetic results and less musculoskeletal injury.

Kim, Ji Eon; Jung, Sung-Ho; Kim, Gwan Sic; Kim, Joon Bum; Choo, Suk Jung; Chung, Cheol Hyun



A new fibrinolytic enzyme (55?kDa) from Allium tuberosum: purification, characterization, and comparison.  


Chives have been used both as food and as medicine. Previously, two fibrinolytic enzymes, ATFE-I (90 kDa) and ATFE-II (55 kDa), were identified in chives (Allium tuberosum), a perennial herb. In the present work, ATFE-II was purified by ion-exchange chromatography followed by gel filtration. In addition, the enzyme properties of ATFE-I and ATFE-II were compared. The molecular mass and isoelectric point (pI value) of ATFE-II were 55 kDa and pI 4.0, respectively, as revealed using one- or two-dimensional fibrin zymography. ATFE-II was optimally active at pH 7.0 and 45°C. ATFE-II degraded the A?-chain of human fibrinogen but did not hydrolyze the B?-chain or the ?-chain, indicating that the enzyme is an ?-fibrinogenase. The proteolytic activity of ATFE-II was completely inhibited by 1 mM leupeptin, indicating that the enzyme belongs to the cysteine protease class. ATFE-II was also inhibited by 1 mM Fe²(+). ATFE-II exhibited high specificity for MeO-Suc-Arg-Pro-Tyr-p-nitroaniline (S-2586), a synthetic chromogenic substrate of chymotrypsin. Thus proteolytic enzymes from A. tuberosum may be useful as thrombolytic agents. PMID:20954802

Chung, Dong-Min; Choi, Nack-Shick; Chun, Hyo Kon; Maeng, Pil Jae; Park, Sang-Bong; Kim, Sung-Ho



Identification of a novel 19 kDa Echinococcus granulosus antigen.  


By screening an Echinococcus granulosus cDNA library with IgG4 from patients with active cystic echinococcosis (CE), we identified a cDNA encoding a protein of 19.0 kDa (Eg19). Eg19, in 12% SDS-PAGE in reducing and non-reducing conditions, showed several bands between 19 and 100 kDa. Immunoblotting (IB) analysis detected total IgG, IgG1 and IgG4 specific to the 38/40 kDa band of Eg19 in the 10% of patients' sera. The percentage of total IgG, IgG1 and IgG4-positive sera were significantly higher in sera from patients with active disease and cyst in multiple sites than from patients with inactive disease and cyst in the liver (P<10(-4)). ELISA analysis disclosed that during the follow-up anti-Eg19 antibody concentration decreased over the course of treatment in sera from patients with cured disease. Even if Eg19 appear to have no benefit in the diagnosis of the disease, our data, confirming the presence of antigens inducing both IgG1 and IgG4 during active development of CE, suggest that Eg19 might be a marker of disease status. PMID:19769934

Delunardo, Federica; Ortona, Elena; Margutti, Paola; Perdicchio, Maurizio; Vacirca, Davide; Teggi, Antonella; Sorice, Maurizio; Siracusano, Alessandra



A 23-kDa protein as a substrate for protein kinase C in bovine neutrophils. Purification and partial characterization  

Microsoft Academic Search

In ³²P{sub i}-loaded bovine neutrophils stimulated with phorbol myristate acetate (PMA), radioactivity was preferentially incorporated into a protein of low molecular mass, suggesting a PKC-dependent phosphorylation. This protein, termed 23-kDa protein, was predominantly localized in the cytosol. The apparent molecular mass of the purified protein range between 20 and 23 kDa. In the absence of mercaptoethanol, a dimer accumulated. Homogeneity

Marie Jose Stasia; Anne Christine Dianoux; Pierre V. Vignais



Subcellular localization of the 28 kDa protein of the triple- gene-block of bamboo mosaic potexvirus  

Microsoft Academic Search

Open reading frame 2 of the bamboo mosaic potexvirus (BaMV) genome encodes a 28 kDa pro- tein, the first of the 'triple-gene-block' of BaMV which is believed to play a role in cell-to-cell movement of the virus in host plants. The 28 kDa protein was expressed in Escherichia coli and polyclonal antiserum was raised in a rabbit. Western blot analyses

Ban-Yang Chang; Na-Sheng Lin; Dann-Ying Liou; Jia-Perng Chen; Gunn-Guang Liou; Yau-Heiu Hsu


Relocalization of an 82-kDa protein from lampbrush loops into the nucleoskeleton during amphibian oogenesis  

Microsoft Academic Search

Summary Affinity-purified antibodies directed against an 82-kDa oocyte nuclear protein ofPleurodeles waltl (Amphibia, Urodela) were prepared using antigen bound to nitrocellulose paper. The specificity of the antibody was controlled on two-dimensional electrophoretic gels of nuclear proteins. The intranuclear distribution of the 82-kDa protein was analyzed by the indirect immunofluorescence method on spreads of oocyte nuclear content. Localization of the protein

Nicole Moreau; Nicole Angelier; Nicole Lautredou



The Portuguese naturalist Correia da Serra (1751–1823) and his impact on early nineteenth-century botany  

Microsoft Academic Search

This paper focuses on the contributions to natural history, particularly in methods of plant classification of the Portuguese\\u000a botanist, man of letters, diplomat, and Freemason Abbé José Correia da Serra (1751–1823), placing them in their national and\\u000a international political and social contexts.\\u000a \\u000a Correia da Serra adopted the natural method of classification championed by the Frenchman Antoine-Laurent de Jussieu, and\\u000a introduced

Maria Paula Diogo; Ana Carneiro; Ana Simões



Purification, immunological characterization and cDNA cloning of a 47 kDa glycoprotein secreted by carrot suspension cells  

Microsoft Academic Search

A 47 kDa glycoprotein, termed EP4, was purified from carrot cell suspension culture medium. An antiserum raised against EP4 also recognized a protein of 45 kDa that was ionically bound to the cell wall. EP4 was detected in culture media from both embryogenic and non-embryogenic cell lines and was found to be secreted by a specific subset of non-embryogenic cells.

Fred A. van Engelen; Anke J. de Jong; Ellen A. Meijer; Cor W. Kuil; J. Kees Meyboom; Wim G. Dirkse; Hilbert Booij; Marijke V. Hartog; Joël Vandekerckhove; Sacco C. de Vries; Ab van Kammen



Identification and purification of a novel 120-kDa protein that recognizes the cAMP-responsive element  

SciTech Connect

The TGACGTCA (CRE) motif required for function by a number of cellular (somatostatin, enkephalin, alpha-human chorionic gonadotropin) and viral (Ad5 E1A-inducible, HTLV-1 TAX-inducible) genes is the site of interaction of multiple sequence-specific complexes. A protocol has been developed for the fractionation and purification of these activities. We report here the purification from HeLa nuclear extracts of a novel 120-kDa polypeptide which by Southwestern blots, gel retardation, and UV cross-linking assays displays CRE-specific binding. The CRE-affinity purified 120-kDa protein displays properties distinct from those of the 43-kDa CREB/ATF polypeptide. The 120-kDa protein is readily phosphorylated in vitro by protein kinase C but not by protein kinase A, suggesting that this molecule may mediate cellular signals distinct from the cAMP-responsive pathway. In vitro transcription-complementation assays utilizing the purified 120-kDa protein failed to transactivate the cAMP-responsive somatostatin promoter suggesting that the mode of action of this 120-kDa polypeptide may require an activation step distinct from the cAMP-signaling pathway.

Andrisani, O.; Dixon, J.E. (Purdue Univ., West Lafayette, IN (USA))



Projeto educação em ciências com observatórios virtuais: a participação da Escola Moppe no período 2000-2003  

NASA Astrophysics Data System (ADS)

O projeto Educação em Ciências com Observatórios Virtuais foi concebido pelo Instituto Astronômico e Geofísico da USP, agregando diversas instituições de ensino e pesquisa no país para desenvolver competências diversas na educação fundamental, média e superior utilizando a astronomia como ferramenta multidisciplinar. Este trabalho descreve a participação da MOPPE, escola-piloto do INPE no projeto, no período de 2000-2003. Serão apresentadas 1) a criação de um clube de ciências (1999 a 2001) cujo tema foi a colonização de Marte e 2) a ementa de astronomia trabalhada com as 7a. e 8a. séries do ensino fundamental. A proposta do projeto Colonizando Marte foi estudar diversos aspectos de uma missão interplanetária e construir experiementos que permitissem quantificar esses aspectos. Os resultados obtidos incluiram apresentações nas SBPC Jovem em 2000 e 2001. Discutiremos também as ementas de astronomia trabalhadas desde 2001 e o envolvimento dos alunos com atividades ligadas a astronomia, fora da aula de ciências. A inclusão de astronomia no currículo das últimas séries motivou a participação de mais alunos culminando com a conquista de duas medalhas para alunos da 7a. série na Olimpiada Brasileira de Astronomia em 2002. Houve também um aumento no número de participantes na OBA 2003 e nos projetos de astronomia mais elaborados nas Feira de Ciências de 2001 e 2002. Destacamos em 2003 a inclusão da MOPPE no projeto TIE - Telescopes in Education - da NASA, que utiliza o telescópio do Observatório de Mount Wilson (EUA) para observações remotas em projetos pedagógicos para o ensino de astronomia.

Wuensche, C. A.; Gavioli, E.; Oliveira, A. L. P. R. S.; da Silva, C.; Cardoso, H. P.; Estácio, S.



Effect of DA-8159, a Selective Phosphodiesterase Type 5 Inhibitor, on Electroretinogram and Retinal Histology in Rabbits  

PubMed Central

DA-8159, a selective inhibitor of phosphodiesterase type 5, was developed as a new drug for erectile dysfunction. The effect of DA-8159 on the electroretinogram (ERG) and the retinal histopathology were evaluated in rabbits. The ERG was performed prior to, and 1 and 5 hr after DA-8159 (5 to 30 mg/kg) administration. The plasma concentration of DA-8159 was determined at each time point, and retinal microscopic examination was also performed. There was no statistically significant ERG change at any dose or at any time. Though the 30 Hz flicker showed a prolongation of the implicit time at 5 hr after the administration of either DA-8159 15 mg or 30 mg/kg (p<0.05), but concurrent amplitude decreases were not statistically significant. At a dose of 5 mg/kg, no test drug was detected in the blood after either 1 or 5 hr. At either 15 mg/kg or 30 mg/kg, there was a dose-dependent increase in the blood concentration after 1 hr of drug administration, which decreased with time. In light and electron microscopic examinations of the retina, there was no remarkable change at any dose. These results suggest DA-8159 has a low risk potential to the retina, but further evaluation on the visual functions in human is needed.

Kang, Kyung Koo; Ahn, Gook Jun; Shim, Hyun Joo; Kim, Won Bae



Análise Temporal da Coma de CO+ no Cometa P/Halley  

NASA Astrophysics Data System (ADS)

Observações fotográficas e fotoelétricas da coma de gás ionizado do cometa P/Halley a nível de CO+ em 4250 angstroms fizeram parte do programa de Monitoramento do Halley desenvolvido pela Universidade de Bochum (Ruhr-Universitaet Bochum) na Alemanha, realizado de 17 de fevereiro a 17 de abril de 1986 no European Southern Observatory (ESO) em La Silla (Chile). Nesta faixa espectral é possível observar a contínua formação, bem como o movimento e expansão das estruturas de plasma. Para observar a morfologia destas estruturas foram analisadas 32 placas fotográficas de CO+ (placas de vidro) do cometa P/Halley. Tais placas possuem um campo de visão de 28,6 por 28,6 graus sendo obtidas entre 29 de março e 17 de abril de 1986 com tempos de exposição entre 20 e 120 minutos. Todas as placas foram digitadas com o auxílio de um microdensitômetro PDS 2020 GM (Photometric Data System) do Instituto Astronómico da Westfaelischen Wilhelms-Universitaet em Muenster, Alemanha (um pixel = 25 por 25 micrômetros correspondendo aproximadamente a 46,88 por 46,88 segundos de arco). Após a digitação os dados foram reduzidos à intensidades relativas, sendo que os posssíveis de calibração também foram reduzidos à intensidades absolutas, expressas em termos de densidade colunar utilizando-se dos sistemas de tratamento de imagens MIDAS (Munich Image Data Analysis System; ESO - Image Processing Group, 1988) e IHAP (Image Handling And Processing; Middleburg, 1983). Com o auxílio do método de teta mínimo de Stellingwerf (Stellingwerf, 1978) obteve-se um período de 2,22 +/- 0,09 dias a partir da análise de estruturas na coma de plasma através da subtração de imagens subsequentes. Este método foi comparado com o método de Fourier. Provavelmente exista um segundo ciclo com período aproximado de 3,6 dias. A idéia de subtrair imagens subsequentes é devido ao fato de que os efeitos de rotação são apenas 10% dos fenômenos de distribuição gasosa. Portanto as imagens de diferença são usadas para suprimir a componente estática da nuvem de gás. - ESO - Image Processing Group, 1988, in: MIDAS Manual, ESO, Garching. - Middleburg F., 1983, in: IHAP Manual, ESO, Garching. - Stellingwerf R.F., 1978, ApJ 224, 953.

Voelzke, M. R.; Schlosser, W.; Schmidt-Kaler, Th.



The Greek Database of Seismogenic Sources (GreDaSS): the new version.  

NASA Astrophysics Data System (ADS)

The Greek Database of Seismogenic Sources (GreDaSS) is a repository of geological, tectonic and active fault data for the Greek territory and its surroundings including sectors of western Anatolia, southern Bulgaria, FYR of Macedonia and Albania. The principal aim of this international project is to create a homogenized framework of all data relevant to the seismotectonics, and especially the seismic hazard assessment, of Greece and its surroundings as well as a common research platform for performing seismic hazard analyses, modelling and scenarios from specific seismogenic structures. GreDaSS represents an on-going project based on the results of decades of investigations by the GreDaSS Working Group* and by numerous other researchers working on the active tectonics of the broader Aegean Region. Members of the WG have contributed in several national and European projects like FAUST (Faults as a seismological tool, 1998-2000) and SHARE (Seismic Hazard and Armonization in Europe, 2009-2012). The database is continuously updated by the WG, with the aim of improving its completeness in terms of seismogenic sources analysed and included, but also of enriching and better defining all parametric and ancillary information on each source. Preliminary and partial versions of GreDaSS have been already presented at previous congresses (Pavlides et al., 2010; Caputo et al., 2012; Sboras et al., 2013), but at next EGU the whole area and the most recent version of the database will be displayed. *) the principal scientific contributors belonging to the GreDaSS WG are: Sboras. S., Chatzipetros A., Koukouvelas I., Michailidou A., Valkaniotis S., Tarabusi G. Caputo R., Chatzipetros A., Pavlides S. and Sboras S. (2012): Ann. Geophysics, 55(5), 859-894, doi: 10.4401/ag-5168 Pavlides S., Caputo R., Sboras S., Chatzipetros A., Papathanasiou G. & Valkaniotis S. (2010): Bull. Geol. Soc. Greece, 43(1), 486-494 Sboras S., Pavlides S., Caputo R., Chatzipetros A., Michailidou A., Valkaniotis S. and Papathanasiou G. (2013): Boll. Geofis. Teor. Appl., 13 pp. doi: 10.4430/bgta0101.

Caputo, Riccardo; Pavlides, Spyros; GreDaSS Working Group



Encompassing the Globe: Portugal and the World in the 16th and 17th Centuries  

NSDL National Science Digital Library

This exhibition from the Smithsonian's Sackler Gallery traces the importance of Portugal in the 16th and 17th Centuries, a time period often called the Age of Discovery. Explorers from Portugal traversed the globe, bringing back news of exotic people, animals, foods, and spices to Europe. The web exhibition highlights Portuguese voyages to Africa, Brazil, China, and Japan, and around the Indian Ocean. By simply following a few links, it is possible to view the exhibition in Google Earth, free software that will plot the routes of the Portuguese explorers, along with a chronology, as well as display the informative captions prepared for the exhibition. Images are also included, such as a salt container, heliotrope spoon, and an ivory casket or box, all from Sri Lanka, the source of cinnamon to the thriving spice trade that Vasco da Gama found when he sailed his four ships into the Indian Ocean in late 1497.



Nuclear 82-kDa choline acetyltransferase decreases amyloidogenic APP metabolism in neurons from APP/PS1 transgenic mice.  


Alzheimer disease (AD) is associated with increased amyloidogenic processing of amyloid precursor protein (APP) to ?-amyloid peptides (A?), cholinergic neuron loss with decreased choline acetyltransferase (ChAT) activity, and cognitive dysfunction. Both 69-kDa ChAT and 82-kDa ChAT are expressed in cholinergic neurons in human brain and spinal cord with 82-kDa ChAT localized predominantly to neuronal nuclei, suggesting potential alternative functional roles for the enzyme. By gene microarray analysis, we found that 82-kDa ChAT-expressing IMR32 neural cells have altered expression of genes involved in diverse cellular functions. Importantly, genes for several proteins that regulate APP processing along amyloidogenic and non-amyloidogenic pathways are differentially expressed in 82-kDa ChAT-containing cells. The predicted net effect based on observed changes in expression patterns of these genes would be decreased amyloidogenic APP processing with decreased A? production. This functional outcome was verified experimentally as a significant decrease in BACE1 protein levels and activity and a concomitant reduction in the release of endogenous A?1-42 from neurons cultured from brains of AD-model APP/PS1 transgenic mice. The expression of 82-kDa ChAT in neurons increased levels of GGA3, which is involved in trafficking BACE1 to lysosomes for degradation. shRNA-induced decreases in GGA3 protein levels attenuated the 82-kDa ChAT-mediated decreases in BACE1 protein and activity and A?1-42 release. Evidence that 82-kDa ChAT can enhance GGA3 gene expression is shown by enhanced GGA3 gene promoter activity in SN56 neural cells expressing this ChAT protein. These studies indicate a novel relationship between cholinergic neurons and APP processing, with 82-kDa ChAT acting as a negative regulator of A? production. This decreased formation of A? could result in protection for cholinergic neurons, as well as protection of other cells in the vicinity that are sensitive to increased levels of A?. Decreasing levels of 82-kDa ChAT due to increasing age or neurodegeneration could alter the balance towards increasing A? production, with this potentiating the decline in function of cholinergic neurons. PMID:24844149

Albers, Shawn; Inthathirath, Fatima; Gill, Sandeep K; Winick-Ng, Warren; Jaworski, Ewa; Wong, Daisy Y L; Gros, Robert; Rylett, R Jane



Structure of yeast pGKL 128-kDa killer-toxin secretion signal sequence. Processing of the 128-kDa killer-toxin-secretion-signal-alpha-amylase fusion protein.  


The linear double-stranded DNA plasmid pGKL1 in yeast encodes a killer toxin consisting of 97-kDa, 31-kDa and 28-kDa subunits. A 128-kDa protein precursor of the 97-kDa and 31-kDa subunits, was first synthesized with a 29-amino-acid extension at its NH2-terminus as a secretion signal sequence. In the present study, the property of this signal sequence was studied by the analysis of a fusion protein with mouse alpha-amylase. Using the secretion signal sequence of the killer protein, the mouse alpha-amylase was successfully secreted into the culture medium. An intracellular precursor form of alpha-amylase was identified and purified. Analysis of the NH2-terminal sequence of this precursor molecule indicated that it corresponded to the secretory intermediate (pro form) of alpha-amylase with the removal of the hydrophobic segment (Met1-Gly16) of the secretion signal. Both the secretion of alpha-amylase into the culture medium and the detection of the pro-alpha-amylase species in the cells were prohibited by a sec 11 mutation, or by the conversion of Gly to Val at the 16th position of the secretion signal. These results strongly suggest that the cleavage occurs between Gly16 and Leu17 by a signal peptidase, and that this cleavage is required for the secretion of alpha-amylase into the medium. Based on the data from the NH2-terminal amino acid sequences of secreted alpha-amylases, we conclude that the 29-amino-acid secretion signal present in the 128-kDa killer toxin precursor protein is a prepro structure. PMID:1735428

Tokunaga, M; Kawamura, A; Omori, A; Hishinuma, F



Relationship of two ribonucleases with molecular masses of 45 kDa and 37 kDa from the culture medium of Lentinus edodes.  


Lentinus edodes (shiitake) produces three base non- specific and acid ribonucleases, RNase Le2, RNase Le37 and RNase Le45. The primary structures of the former two RNases, having molecular masses about 24 and 37 kDa, respectively, have been elucidated to be members of the RNase T2 family. The latter two are excreted from mycelia into the medium. In this report, we estimated the primary structure of RNase Le45 using the following experimental evidence. (i) The partial amino acid sequence of RNase Le45 determined that up to about 60% of total protein was identical with that of RNase Le37. (ii) The amino acid composition of RNase Le45 was identical to that of RNase Le37. (iii) The elution profiles on HPLC of lysylendopeptidase and Staphylococcus aureus V8 protease digests of RCM-RNase Le45 (reduced and S-carboxymethylated RNase Le45) were very similar to those of RNase Le37, except for the absence of C-terminus peptide which contained O-glycosylated peptides. However, RNase Le45 contained about 70 residues of mannose and 4 residues of hexosamine. These values were more than twice those of RNase Le37. (iv) RNase Le45 was immunologically indistinguishable from RNase Le37. (v) After treatment with both glycosidase EndoH and alpha-mannosidase, RNases Le37 and Le45 gave complex bands by slab-gel electrophoresis. However, one of the major bands with the highest mobility from RNase Le45 and Le37 showed the molecular mass of 29 kDa in common, which is slightly larger than that of RNase Le2 containing no carbohydrate. These results indicated that RNase Le45 is an enzyme which is a heavily glycosylated species of RNase Le37. PMID:10919355

Kobayashi, H; Hara, J; Itagaki, T; Inokuchi, N; Koyama, T; Sanda, A; Iwama, M; Ohgi, K; Irie, M



Assessment of WSSV prevalence and distribution of disease-resistant shrimp among the wild population of Penaeus monodon along the west coast of India.  


Shrimp aquaculture is threatened by many diseases, among which white spot disease (WSD) caused by white spot syndrome virus (WSSV) is the leading one. Information related to the geographical distribution and seasonal prevalence of WSD is necessary to obtain a clear understanding of the disease biology in shrimp. Identification of WSD-resistant individual shrimp with DNA markers is also an important technique to develop better WSD-free shrimp health management. The present study aim is to estimate the occurrence of WSSV in Penaeus monodon qualitatively and quantitatively during three different seasons during the years 2011 to 2013 along the west coast of India. Additionally, the disease resistance prevalence using previously developed 71 bp microsatellite and 457 bp RAPD-SCAR DNA markers is also investigated. Samples were collected throughout the year from four locations along the west coast of India: Kochi, Kerala; Mangalore, Karnataka; Vasco-da-Gama, Goa; and Veraval, Gujarat. The results depicted that the average WSSV prevalence, as determined by the nested PCR method and taken cumulatively over the four locations, was the lowest (0%) during the post-monsoon season and the highest (31.6%) during the monsoon season. The WSD prevalence was observed to increase when the latitude was decreased along the west coast of India (from Veraval to Kochi). Out of the three different seasons, the average WSSV copy number was the highest (approximately 10(3) copies ?g(-1) shrimp genomic DNA) during the monsoon season. The disease-resistant prevalence, as determined using the developed DNA markers, was found to be the highest in Vasco-da-Gama (59.5%) and the lowest in Kochi (40.9%). The present study suggests better options for the efficient collection of disease-free and disease-resistant brood stocks, which would be a more cost-effective and safer approach toward disease prevention over conventional trends of seed generation from unselected wild brood stock. PMID:24681359

Chakrabarty, Usri; Mallik, Ajoy; Mondal, Debabrata; Dutta, Sourav; Mandal, Nripendranath



Analyzing the relation between the microbial diversity of DaQu and the turbidity spoilage of traditional Chinese vinegar.  


Vinegar is a traditional fermented condiment, and the microbial diversity of DaQu makes the quality of vinegar products. Recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. In this study, the relation between the microbial diversity of DaQu and turbidity spoilage of vinegar was analyzed by plating technique, PCR-denaturing gradient gel electrophoresis (DGGE), and high-performance liquid chromatography (HPLC). The 16S rRNA sequencing and DGGE analysis indicated that Bacillus (Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus thuringiensis) and Lactobacillus (including Lactobacillus acidipiscis and Lactobacillus pobuzihii) species were the dominant contaminants in vinegar products. Meanwhile, DGGE analysis showed that the dominant bacteria in DaQu belonged to genera Bacillus, Lactobacillus, Pseudomonas, Weissella, Saccharopolyspora, Enterobacter, and Pantoea. However, only two yeast species (Pichia kudriavzevii and Saccharomycopsis fibuligera) and seven mold species including Aspergillus oryzae, Aspergillus niger, Aspergillus candidus, Rhizopus microspores, Eurotium herbariorum, Absidia corymbifera, and Eupenicillium javanicum were detected in the DaQu. The population level of fungi was below 5 log CFU/g in DaQu. The chemical and physical properties of vinegar and sediments were also determined. On the basis of a combined microbial diversity-chemical analysis, we demonstrated that turbidity spoilage of vinegar was a result of cooperation among the low population level and abundance of fungal species in DaQu, the suitable climate conditions, and the contaminants in vinegar. This is the first report to analyze the relation between the microbial diversity of DaQu and turbidity spoilage of vinegar. PMID:24691870

Li, Pan; Li, Sha; Cheng, Lili; Luo, Lixin



Heparin Binds 8 kDa Gelsolin Cross-?-Sheet Oligomers and Accelerates Amyloidogenesis by Hastening Fibril Extension†  

PubMed Central

Glycosaminoglycans (GAGs) are highly sulfated linear polysaccharides prevalent in the extracellular matrix, and they associate with virtually all amyloid deposits in vivo. GAGs accelerate the aggregation of many amyloidogenic peptides in vitro, but little mechanistic evidence is available to explain why. Herein, spectroscopic methods demonstrate that GAGs do not affect the secondary structure of the monomeric 8 kDa amyloidogenic fragment of human plasma gelsolin. Moreover, monomerized 8 kDa gelsolin does not bind to heparin under physiological conditions. In contrast, 8 kDa gelsolin cross-?-sheet oligomers and amyloid fibrils bind strongly to heparin, apparently due to electrostatic interactions between the negatively charged polysaccharide and a positively charged region of the 8 kDa gelsolin assemblies. Our observations are consistent with a scaffolding mechanism, whereby cross-?-sheet oligomers, upon formation, bind to GAGs, accelerating the fibril extension phase of amyloidogenesis, possibly by concentrating and orienting the oligomers to more efficiently form amyloid fibrils. Notably, heparin decreases the 8 kDa gelsolin concentration necessary for amyloid fibril formation, likely a consequence of fibril stabilization through heparin binding. Since GAG overexpression, which is common in amyloidosis, may represent a strategy to minimize cross-?-sheet oligomer toxicity by transforming them into amyloid fibrils, the mechanism described herein for GAG-mediated acceleration of 8 kDa gelsolin amyloidogenesis provides a starting point for therapeutic strategy development. The addition of GAG mimetics, small molecule sulfonates shown to reduce the amyloid load in animal models of amyloidosis, to a heparin-accelerated 8 kDa gelsolin aggregation reaction neither significantly alters the rate of amyloidogenesis nor prevents oligomers from binding to GAGs, calling into question their commonly accepted mechanism.

Solomon, James P.; Bourgault, Steve; Powers, Evan T.; Kelly, Jeffery W.



Nitrergic Pathway Is the Major Mechanism for the Effect of DA-9701 on the Rat Gastric Fundus Relaxation  

PubMed Central

Background/Aims DA-9701 significantly improved gastric accommodation by increasing the postprandial gastric volume. In this study, we investigated how DA-9701 affects the rat gastric fundus relaxation. Methods Gastric fundus muscle strips (9 longitudinal and 7 circular muscles) were obtained from rats. Electrical field stimulation (EFS) was performed at various frequencies (1, 5, 10 and 20 Hz) and train durations (1, 5, 10 and 20 seconds) to select optimal condition for experiments. Isometric force measurements were performed in response to EFS. Peak and nadir were observed during the first 1 minute after initiation of EFS in control state and after sequential addition of atropine (1 ?M), DA-9701 (0.5, 5, 25 and 50 ?g), N-nitro-L-arginine (L-NNA, 100 ?M), MRS2500 (1 ?M) and tetrodotoxin (TTX, 1 ?M) to the organ bath. Results The optimal frequency and duration of EFS to evoke nerve-mediated relaxation was determined as 5 Hz for 10 seconds. Addition of L-NNA in the presence of atropine and DA-9701 (50 ?g) decreased nadir by inhibiting relaxation from ?0.054 ± 0.021 g to ?0.022 ± 0.015 g (P = 0.026) in longitudinal muscles. However, subsequent application of MRS2500 in the presence of atropine, DA-9701 (50 ?g) and L-NNA did not affect nadir. In circular muscles, subsequent addition of L-NNA and MRS2500 in the presence of atropine and DA-9701 (50 ?g) did not show significant change of nadir. Conclusions Our data suggest that the effect of DA-9701 on the rat gastric fundus relaxation is mainly mediated by nitrergic rather than purinergic pathway.

Min, Yang Won; Ko, Eun-ju; Lee, Ji Yeon; Min, Byung-Hoon; Lee, Jun Haeng; Kim, Jae J; Rhee, Poong-Lyul



Cloning and expression in Escherichia coli of DNA encoding a 60 kDa stress protein of Mycobacterium paratuberculosis, the causative agent of Johne's disease  

Microsoft Academic Search

Polymerase chain reaction (PCR) was used to generate DNA encoding a 60 kDa stress protein of Mycobacterium paratuberculosis using primers complementary to sequences at the 5' and 3' ends of 60 kDa stress protein genes (encoding the '65 kDa antigens') of M. leprae and M. tuberculosis. The predicted PCR product of 1.8 kb contained the entire coding sequence of an

Angela Colston; Ian McConnell; R. Bujdoso



Vaccination of ducks with recombinant outer membrane protein (OmpA) and a 41 kDa partial protein (P45N?) of Riemerella anatipestifer  

Microsoft Academic Search

The generation of protective immunity against Riemerellaanatipestifer infection in ducks were investigated by immunizations with recombinant glutathione sulfatransferase (GST) fusion’s proteins of OmpA, a 42kDa major outer membrane protein, and P45N?, a 41kDa N-terminal fragment of a newly identified 45kDa potential surface protein from R. anatipestifer. The DNA encoding OmpA and P45N? were isolated from R. anatipestifer serotype 15 (field

Bin Huang; Sumathi Subramaniam; Joachim Frey; Hilda Loh; Hai-Meng Tan; Charlene J Fernandez; Jimmy Kwang; Kim-Lee Chua



A geochemical and geophysical approach to derive a conceptual circulation model of CO 2 -rich mineral waters: A case study of Vilarelho da Raia, northern Portugal  

Microsoft Academic Search

.   The Vilarelho da Raia-Chaves region, located in northern Portugal adjacent to the Spanish border, is characterized by both\\u000a hot and cold CO2-rich mineral waters issuing from springs and drilled wells. The present paper updates the conceptual circulation model of\\u000a the Vilarelho da Raia cold CO2-rich mineral waters. Vilarelho da Raia mineral waters, dominated by Na and HCO3 ions, have

J. M. Marques; Monteiro F. Santos; R. Graça; R. Castro; L. Aires-Barros; L. Mendes Victor



Bullous Pemphigoid and Linear IgA Dermatosis Sera Recognize a Similar 120-kDa Keratinocyte Collagenous Glycoprotein with Antigenic Cross-Reactivity to BP180  

Microsoft Academic Search

Circulating IgG from a large subset of bullous pemphigoid (BP) patients reacted on immunoblot with a 120-kDa protein in conditioned keratinocyte culture medium and in keratinocyte cell extracts. A protein with a similar molecular weight was recognized by circulating IgA from a subset of patients with linear IgA dermatosis (LAD). Both affinity-purified 120- kDa-specific BP IgG and 120-kDa-specific LAD IgA

Hendri H. Pas; Guus J. Kloosterhuis; Klaas Heeres; Jan B. Van der Meer; Marcel F. Jonkman



Antibodies elicited by a virosomally formulated Plasmodium falciparum serine repeat antigen-5 derived peptide detect the processed 47 kDa fragment both in sporozoites and merozoites  

Microsoft Academic Search

Serine repeat antigen-5 (SERA5) is a candidate antigen for inclusion into a malaria subunit vaccine. During merozoite release and reinvasion the 120kDa SERA5 precursor protein (P120) is processed, and a complex consisting of an N-terminal 47kDa (P47) and a C-terminal 18kDa (P18) processing product associates with the surface of merozoites. This complex is thought to be involved in merozoite invasion

Shinji L. Okitsu; Francesca Boato; Markus S. Mueller; Dong Bo Li; Denise Vogel; Nicole Westerfeld; Rinaldo Zurbriggen; John A. Robinson; Gerd Pluschke



The effect of DA8159, a novel PDE5 inhibitor, on erectile function in the rat model of hypercholesterolemic erectile dysfunction  

Microsoft Academic Search

This study examined the effects of a new phosphodiesterase type 5 inhibitor, DA-8159, on erectile function associated with hypercholesterolemia. First of all, in order to investigate whether chronic administration of DA-8159 prevents the development of erectile dysfunction associated with hypercholesterolemia, male SD rats were divided into four groups (normal control, hypercholesterolemic control, DA-8159 5 or 20 mg\\/kg\\/day). Over a 5-month

K K Kang; J Y Yu; M Yoo; J W Kwon



Isolation and Sequence of a cDNA Clone Encoding the 31-kDa Subunit of Bovine Kidney Vacuolar H+ATPase  

Microsoft Academic Search

We have isolated a cDNA encoding the 31-kDa subunit of the bovine kidney vacuolar H+-ATPase. The composite sequence contains 1219 base pairs, which includes the entire 678-base-pair coding region. A lysine-rich sequence previously found in the Na+, K+-ATPase alpha subunit and the H+, K+-ATPase was identified in the 31-kDa subunit. An RNA blot and an immunoblot demonstrated variable 31-kDa subunit

Sheldon Hirsch; Arnold Strauss; Khalid Masood; Stephen Lee; Vikas Sukhatme; Stephen Gluck



Immunoblot evaluation of the 100 and 130 kDa antigens in camel hydatid cyst fluid for the serodiagnosis of human cystic echinococcosis in Libya  

Microsoft Academic Search

Two high molecular weight antigens with molecular masses approximately 100 kDa and 130 kDa were identified by immunoblotting camel hydatid cyst fluid, with 94% sensitivity in sera from surgically confirmed Libyan cystic echinococcosis cases. 40% of sera from surgically confirmed alveolar echinococcosis cases cross-reacted with the 100 and 130 kDa antigens, as did 5·3% of sera from human Taenia solium

M. K. Shambesh; P. S. Craig; A. M. Gusbi; E. F. Echtuish; H. Wen



Purification and spectroscopic properties of 124-kDa oat phytochrome.  


A simplified procedure for the isolation and purification of 124-kDa phytochrome from etiolated Avena seedlings has been developed using the method of ammonium sulfate back-extraction. After hydroxyapatite chromatography of seedling tissue extracts, the pooled phytochrome was subjected to ammonium sulfate back-extraction instead of the usual application to an Affi-Gel Blue column. The resulting phytochrome had specific absorbance ratios (SAR = A666/A280) ranging from 0.85 to 0.95. Subsequent Bio-Gel filtration chromatography yielded highly pure 124-kDa phytochrome with SAR values ranging from 0.99 to 1.13. The absorption maxima of 124-kDa phytochrome were at 280, 379, and 666 nm for the red absorbing form of phytochrome (Pr) and at 280, 400 and 730 nm for the far-red absorbing form (Pfr). The A730/A673 ratio in Pfr was found to be 1.5 to 1.6. The mole fraction of Pfr under red light photoequilibrium was 0.88. No dark reversion was detected within 5 h at 3 degrees C. A photoreversible far-uv-circular dichroism was observable with all phytochrome preparations examined. Fluorescence and phosphorescence lifetimes were measured to further characterize the differences between the phytochromes prepared under different conditions. The Trp fluorescence and phosphorescence lifetimes of Pr and Pfr with the chromophore "X", probably polyphenolic in nature, were significantly shorter than those of phytochrome without the contaminant X. The short lifetime of the fluorescence of the Pr chromophore is attributable to X in the former. PMID:3661984

Chai, Y G; Singh, B R; Song, P S; Lee, J; Robinson, G W



Projeto do sistema anti-ressonante da fiação dos transdutores para o detector Mario Schenberg  

NASA Astrophysics Data System (ADS)

O detector de ondas gravitacionais Mario Schenberg está sendo projetado e construído pelo grupo Gráviton. Sua construção está ocorrendo no Laboratório de Estado Sólido e Baixas Temperaturas (LESBT) da Universidade de São Paulo, na cidade de São Paulo. Esse detector possui uma massa ressonante esférica de cobre-alumínio, com 65 cm de diâmetro, pesando aproximadamente 1150 Kg, suspensa por um sistema de isolamento vibracional, que se encontra em fase de testes preliminares. A real eficácia desse sistema, entretanto, só poderá ser comprovada quando o detector estiver aparelhado com, pelo menos, um transdutor eletromecânico de altíssima sensibilidade acoplado à massa ressonante. Neste momento, não só este sistema de isolamento vibracional será posto em teste, como o do projeto da fiação que transporta os sinais de microondas até os transdutores e destes para a pré-amplificação. Apesar dessa fiação ter sido projetada para não apresentar nenhum contato com a superfície esférica da antena, de maneira a não haver nenhuma transmissão de ruído vibracional do laboratório para esta, deve-se minimizar o ruído microfônico produzido nessa fiação por oscilações mecânicas, uma vez que ela não utiliza nenhum sistema de isolamento vibracional. Com o intuito de resolver este problema, projetamos uma estrutura, formada por pequenos cilindros conectados por barras, a qual não terá nenhuma ressonância mecânica na faixa de freqüências de interesse para detecção (3000 - 3400 Hz). Desta forma, as vibrações nessa faixa não serão amplificadas. O projeto foi feito usando iterativamente, de maneira a otimizar os resultados obtidos, o programa de elementos finitos Msc/Nastran. Através de simulações feitas neste programa, determinamos os parâmetros geométricos ideais a serem utilizados, os quais proporcionam a maior região espectral de interesse livre de ressonâncias.

Vieira, S. J.., Jr.; Melo, J. L.



Purification and spectral studies on the Ca2+-binding properties of 67 kDa calcimedin.  

PubMed Central

The 67 kDa calcimedin, isolated by using a phenyl-Sepharose affinity column followed by DEAE-cellulose and gel-filtration chromatographies, was homogeneous by the criterion of SDS/polyacrylamide-gel electrophoresis. In non-SDS gels, the protein moved faster in the presence of EDTA, suggesting that Ca2+ binding affects its mobility in a manner similar to other Ca2+-binding proteins such as calmodulin and S-100 proteins. The 67 kDa protein underwent a conformational change upon binding Ca2+, as revealed by u.v. difference spectroscopy and near-u.v. c.d. measurements. Tryptophan and tyrosine residues were perturbed upon Ca2+ binding, moving to a more non-polar environment in the presence of Ca2+. Upon excitation of the protein at 280 nm, the fluorescence emission maximum was centered around 325 nm, suggesting that the tryptophan residues are located in a fairly hydrophobic region. Ca2+ addition did not induce a significant change in the intrinsic protein fluorescence intensity at 325 nm. Addition of Ca2+ to the 67 kDa protein labelled with 2-p-toluidinylnaphthalene-6-sulphone (TNS) resulted in a 25% increase in fluorescence intensity, accompanied by a blue shift of the emission maximum from 442 to 432 nm. Hence, the probe in the presence of Ca2+ moves to a more non-polar microenvironment, like calmodulin and other Ca2+-binding proteins. Fluorescence titration with Ca2+ using TNS-labelled protein revealed one class of binding site, with a Kd value of 2 x 10(-5) M. Images Fig. 2.

Mani, R S; Kay, C M



Cloning and expression of human 75-kDa inositol polyphosphate-5-phosphatase.  


Inositol polyphosphate-5-phosphatase (5-phosphatase) hydrolyzes inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate and thereby functions as a signal terminating enzyme in cellular calcium ion mobilization. A cDNA encoding human platelet 5-phosphatase has been isolated by screening for beta-galactosidase fusion proteins that bind to inositol 1,3,4,5-tetrakisphosphate. The sensitivity of the screening procedure was enhanced 50- to 100-fold by amplification of "sublibraries" prior to carrying out binding assays. The sequences derived from the "expression clone" were used to screen human erythroleukemia cell line and human megakaryocytic cell line cDNA libraries. We obtained two additional clones which together consist of 2381 base pairs. The amino-terminal amino acid sequence from the 75-kDa 5-phosphatase purified from platelets is identical to amino acids 38-56 predicted from the cDNA. This suggests that the platelet 5-phosphatase is formed by proteolytic processing of a larger precursor. The cDNA predicts that the mature enzyme contains 635 amino acids (Mr 72, 891). Antibodies directed against recombinant TrpE fusion proteins of either an amino-terminal region or a carboxyl-terminal region immunoprecipitate the enzyme activity from a preparation of the 75-kDa form of platelet 5-phosphatase (Type II) but do not precipitate the distinct 47-kDa 5-phosphatase (Type I) also found in platelets. In addition, the recombinant protein expressed in Cos-7 cells has the same 5-phosphatase activity as the platelet 5-phosphatase. PMID:1718960

Ross, T S; Jefferson, A B; Mitchell, C A; Majerus, P W



Diagnostic potential of 16 kDa (HspX, ?-crystalline) antigen for serodiagnosis of tuberculosis  

PubMed Central

Background & objectives: Tuberculosis (TB) is a public health problem worldwide. Rapid and accurate diagnosis of tuberculosis is crucial to facilitate early treatment of infectious cases and to reduce its spread. The present study was aimed to evaluation of 16 kDa antigen as a serodiagnostic tool in pulmonary and extra-pulmonary tuberculosis patients in an effort to improve diagnostic algorithm for tuberculosis. Methods: In this study, 200 serum samples were collected from smear positive and culture confirmed pulmonary tuberculosis patients, 30 tubercular pleural effusions and 21 tubercular meningitis (TBM) patients. Serum samples from 36 healthy, age matched controls (hospital staff), along with 60 patients with non-tubercular respiratory diseases were also collected and evaluated. Humoral response (both IgG and IgA) was looked for 16 kDa antigen using indirect ELISA. Results: Sensitivity of detection in various categories of pulmonary TB patients ranged between 73.8 and 81.2 per cent. While in the extra-pulmonary TB samples the sensitivity was 42.8 per cent (TBM) and 63.3 per cent (tubercular pleural effusion). The test specificity in both the groups was high (94.7%). All of the non-disease controls were negative. Among non-tubercular disease controls, five patients gave a positive humoral response against 16 kDa. Interpretation & conclusions: Serodiagnostic tests for TB have always had drawbacks of suboptimal sensitivity and specificity. The antigen used in this study gave encouraging results in pulmonary TB only, while in extra-pulmonary TB (tubercular meningitis and tubercular pleural effusion), this has shown a limited role in terms of sensitivity. Further work is required to validate its role in serodiagnosis of TB especially extra-pulmonary TB.

Kaushik, Amit; Singh, Urvashi B.; Porwal, Chhavi; Venugopal, Shwetha J.; Mohan, Anant; Krishnan, Anand; Goyal, Vinay; Banavaliker, Jayant N.



Rectifying performance of D-?-A molecules based on cyanovinyl aniline derivatives  

NASA Astrophysics Data System (ADS)

Using the first-principles method, we investigate rectifying performances of D-?-A molecules based on cyanovinyl aniline derivatives. The calculated results show that different functional groups can change the location of molecular orbitals and thus change the rectifying properties of molecules. Interestingly, we find that although the electronic structure for our studied systems is in agreement with that proposed originally by Aviram and Ratner [Chem. Phys. Lett. 29, 277 (1974)], the rectifying direction is opposite from it due to the asymmetric shift of molecular levels under biases of different polarities. Only for model (M4), it shows a forward rectifying performance under larger bias.

Pan, J. B.; Zhang, Z. H.; Deng, X. Q.; Qiu, M.; Guo, C.



Response to the comments on "The formation of Pangea" by D.A. Ruban  

NASA Astrophysics Data System (ADS)

The Variscan structures of the Caucasus region are still quite difficult to decipher, they certainly deserved some in depth investigations in the future. Thus, it is right to question any paleogeographic models proposed in that area, as made by D.A. Ruban. We present here the arguments that we used to decide on the distribution of the terranes in that region. The Transcaucasus massif is regarded as pertaining to the Galatian super-terrane, whereas, the Great Caucasus terrane belongs to the Hanseatic ribbon terrane. The latter was a part of Hunia, detached from Laurussia in the Devonian.

Stampfli, Gérard M.



The strainrange partitioning behavior of an advanced gas turbine disk alloy, AF2-1DA  

NASA Technical Reports Server (NTRS)

The low-cycle, creep-fatigue characteristics of the advanced gas turbine disk alloy, AF2-1DA have been determined at 1400 F and are presented in terms of the method of strainrange partitioning (SRP). The mean stresses which develop in the PC and CP type SRP cycles at the lowest inelastic strainrange were observed to influence the cyclic lives to a greater extent than the creep effects and hence interfered with a conventional interpretation of the results by SRP. A procedure is proposed for dealing with the mean stress effects on life which is compatible with SRP.

Halford, G. R.; Nachtigall, A. J.



p56lck stably associates with a 115 kDa substrate.  


Using antibodies directed against p56lck, we have identified a 115 kDa protein (p115) that is specifically immunoprecipitated with p56lck from whole cell lysates. The p56lck/p115 complex is stable in the presence of nonionic detergents. p115 becomes phosphorylated on tyrosine residues in p56lck immune-complex kinase assays. Treatment of whole cells with 12-O-tetradecanoyl phorbol-13-acetate decreases the subsequent tyrosine phosphorylation of p115 in immune-complex kinase assays. PMID:1632788

Srinivas, P R; Hutchcroft, J E; Aube, B D; Harrison, M L



Utilities for Quantifying Separation in PCA/PLS-DA Scores Plots  

PubMed Central

Metabolic fingerprinting studies rely on interpretations drawn from low-dimensional representations of spectral data generated by methods of multivariate analysis such as PCA and PLS-DA. The growth of metabolic fingerprinting and chemometric analyses involving these low-dimensional scores plots necessitates the use of quantitative statistical measures to describe significant differences between experimental groups. Our updated version of the PCAtoTree software provides methods to reliably visualize and quantify separations in scores plots through dendrograms employing both nonparametric and parametric hypothesis testing to assess node significance, as well as scores plots identifying 95% confidence ellipsoids for all experimental groups.

Worley, Bradley; Halouska, Steven; Powers, Robert



Study of the hadronic interactions of kaons in light nuclei at DA?ne  

NASA Astrophysics Data System (ADS)

The AMADEUS experiment at the Da?ne accelerator of the Frascati National Laboratories (Italy) of INFN, will perform, for the first time, full-acceptance studies of antikaon interaction in light nuclei, with a complete experimental program for the case of the kaonic clusters. Studying the absorption of antikaon by the nucleus will provide information concerning the KN interaction and the modification of the kaon mass in the nuclear medium. A preliminar study of these kind of hadronic interactions is being done by the AMADEUS collaboration by analyzing the existent KLOE data.

Vazquez Doce, O.



Towards a Standardized Line List for G 191-B2B and other DA Type Objects  

NASA Astrophysics Data System (ADS)

We present a comprehensive analysis of the far UV spectrum of G 191-B2B over the range of 900-1700Å using co-added data from the FUSE and STIS archives. While previous identifications made by Holberg et al. (2003) are reaffirmed in this work, it is found that many previously unidentified lines can now be attributed to Fe, Ni, and a few lighter metals. Future work includes extending this detailed analysis to a wider range of DA objects, in the expectation that a more complete analysis of their atmospheres can be realised.

Preval, S. P.; Barstow, M. A.; Holberg, J. B.; Dickinson, N. J.



Role of the 37 kDa laminin receptor precursor in the life cycle of prions.  


Prions are thought to consist of infectious proteins that cause, in the absence of detectable nucleic acid, a group of fatal neurodegenerative diseases, called transmissible spongiform encephalopathies (TSE). Among these diseases are bovine spongiform encephalopathy (BSE), scrapie of sheep and Creutzfeldt-Jakob disease (CJD) in humans. They occur as sporadic, infectious or genetic disorders and have in common the accumulation of an abnormal, pathogenic isoform of the cellular prion protein PrPc which is converted in a post-translational process into PrPSc concomitant with conformational changes of the protein. During this process PrPc acquires a high beta-sheet content and becomes partially resistant to proteases. The mechanism of this conversion as well as the physiological function of the cellular prion protein PrPc are poorly understood, but studies employing PrP knock-out mice demonstrated that PrPc is required for the development of prion diseases. The involvement of co-factors such as chaperones, receptors or an unknown protein, designated "protein X" in the conversion process are discussed. In a yeast two-hybrid screen we have identified the 37 kDa laminin receptor precursor (LRP) as an interactor of the cellular prion protein and this interaction could be confirmed by co-infection and co-transfection studies in mammalian and insect cells. LRP evolved from the ribosomal protein p40 essential for protein synthesis lacking any laminin binding activity to a cell surface receptor binding laminin, elastin and carbohydrates. The gene encoding 37 kDa LRP/p40 has been identified in a variety of species including the sea urchin Urechis caupo, Chlorohydra viridissima, the archaebacterium Haloarcula marismortui, the yeast Saccharomyces cerevisiae as well as in mammals where it is highly conserved. LRP works as a receptor for alphaviruses and is associated with the metastatic potential of solid tumors where it was first identified. The 37 kDa LRP forms its mature 67 kDa isoform with high laminin binding capacity by an unknown mechanism involving acylation. The multifunctionality of LRP as a ribosomal protein and a cell surface receptor for infectious agents such as viruses and prions might be extended by additional properties. PMID:10188208

Rieger, R; Lasmézas, C I; Weiss, S



Structural health monitoring of the church of Santa Casa da Misericordia of Aveiro using FBG sensors  

NASA Astrophysics Data System (ADS)

This paper presents a structural health monitoring system, based on fibre Bragg gratings, developed for the church of Santa Casa da Misericordia of Aveiro. This system comprises 19 displacement sensors and 5 temperature sensors. All the sensors were custom made according to the monitoring points' characteristics. The results obtained over the first months are presented. The objective of this work is to gather data that will bring a deeper knowledge of how this structure behaves and to help planning the recovering interventions in this historical building.

Lima, H. F.; Vicente, R.; Nogueira, R. N.; Abe, I.; André, P.; Fernandes, C.; Rodrigues, H.; Varum, H.; Kalinowski, H. J.; Costa, A.; Pinto, J. L.



Pharmacological profile of DA-6886, a novel 5-HT4 receptor agonist to accelerate colonic motor activity in mice.  


DA-6886, the gastrointestinal prokinetic benzamide derivative is a novel 5-HT4 receptor agonist being developed for the treatment of constipation-predominant irritable bowel syndrome (IBS-C). The purpose of this study was to characterize in vitro and in vivo pharmacological profile of DA-6886. We used various receptor binding assay, cAMP accumulation assay, organ bath experiment and colonic transit assay in normal and chemically constipated mice. DA-6886 exhibited high affinity and selectivity to human 5-HT4 receptor splice variants, with mean pKi of 7.1, 7.5, 7.9 for the human 5-HT4a, 5-HT4b and 5-HT4d, respectively. By contrast, DA-6886 did not show significant affinity for several receptors including dopamine D2 receptor, other 5-HT receptors except for 5-HT2B receptor (pKi value of 6.2). The affinity for 5-HT4 receptor was translated into functional agonist activity in Cos-7 cells expressing 5-HT4 receptor splice variants. Furthermore, DA-6886 induced relaxation of the rat oesophagus preparation (pEC50 value of 7.4) in a 5-HT4 receptor antagonist-sensitive manner. The evaluation of DA-6886 in CHO cells expressing hERG channels revealed that it inhibited hERG channel current with an pIC50 value of 4.3, indicating that the compound was 1000-fold more selective for the 5-HT4 receptor over hERG channels. In the normal ICR mice, oral administration of DA-6886 (0.4 and 2mg/kg) resulted in marked stimulation of colonic transit. Furthermore, in the loperamide-induced constipation mouse model, 2mg/kg of DA-6886 significantly improved the delay of colonic transit, similar to 10mg/kg of tegaserod. Taken together, DA-6886 is a highly potent and selective 5-HT4 receptor agonist to accelerate colonic transit in mice, which might be therapeutic agent having a favorable safety profile in the treatment of gastrointestinal motor disorders such as IBS-C and chronic constipation. PMID:24769304

Lee, Min Jung; Cho, Kang Hun; Park, Hyun Min; Sung, Hyun Jung; Choi, Sunghak; Im, Weonbin



New paleomagnetic constraints on the extrusion of Indochina: Late Cretaceous results from the Song Da terrane, northern Vietnam  

NASA Astrophysics Data System (ADS)

Samples were collected for paleomagnetic investigations from the Upper Cretaceous Yen Chau Formation in the Song Da terrane (21.7°N, 103.9°E) bounded by the Ailao Shan-Red River fault system and Song Ma fault, in an attempt to examine extrusion tectonics of East Asia. Primary nature of the high-temperature (600-690 °C) component of magnetization from 13 sites is supported by a positive fold test. The tectonic corrected data provides the characteristic Late Cretaceous paleomagnetic direction for the Song Da terrane ( D=6.4°, I=32.0° with ?95=8.5°, N=13 sites), corresponding to a paleopole lying at 82.9°N, 220.7°E ( A95=6.9°). Comparison with coeval paleomagnetic poles for the neighboring tectonic blocks indicates no latitudinal translation of the Son Da terrane with respect to the South China Block, whereas the Shan-Thai Block and the southern part of the Indochina Block revealed a southward displacement of 10.5±9.5° in latitude with respect to the Song Da terrane. We conclude that the southeastern segment of the Ailao Shan-Red River fault system to the east of the Dien Bien Phu fault is not a demarcation of the extruded Indochina Peninsula. The Indochina Block extruded probably along some faults between the Song Da terrane and the Khorat Plateau.

Takemoto, Kazuhiro; Halim, Nadir; Otofuji, Yo-ichiro; Van Tri, Tran; Van De, Le; Hada, Shigeki



Binding of TFIID and MEF2 to the TATA element activates transcription of the Xenopus MyoDa promoter.  

PubMed Central

Members of the MyoD family of helix-loop-helix proteins control expression of the muscle phenotype by regulating the activity of subordinate genes. To investigate processes that control the expression of myogenic factors and regulate the establishment and maintenance of the skeletal muscle phenotype, we have analyzed sequences necessary for transcription of the maternally expressed Xenopus MyoD (XMyoD) gene. A 3.5-kb DNA fragment containing the XMyoDa promoter was expressed in a somite-specific manner in injected frog embryos. The XMyoDa promoter was active in oocytes and cultured muscle cells but not in fibroblasts or nonmuscle cell lines. A 58-bp fragment containing the transcription initiation site, a GC-rich region, and overlapping binding sites for the general transcription factor TFIID and the muscle-specific factor MEF2 was sufficient for muscle-specific transcription. Transcription of the minimal XMyoDa promoter in nonmuscle cells was activated by expression of Xenopus MEF2 (XMEF2) and required binding of both MEF2 and TFIID to the TATA motif. These results demonstrate that the XMyoDa TATA motif is a target for a cell-type-specific regulatory factor and suggests that MEF2 stabilizes and amplifies XMyoDa transcription in mesodermal cells committed to the muscle phenotype. Images

Leibham, D; Wong, M W; Cheng, T C; Schroeder, S; Weil, P A; Olson, E N; Perry, M



Agarose and Polyacrylamide Gel Electrophoresis Methods for Molecular Mass Analysis of 5-500 kDa Hyaluronan  

PubMed Central

Agarose and polyacrylamide gel electrophoresis systems for the molecular mass-dependent separation of hyaluronan (HA) in the size range of approximately 5–500 kDa have been investigated. For agarose-based systems, the suitability of different agarose types, agarose concentrations, and buffers systems were determined. Using chemoenzymatically synthesized HA standards of low polydispersity, the molecular mass range was determined for each gel composition, over which the relationship between HA mobility and logarithm of the molecular mass was linear. Excellent linear calibration was obtained for HA molecular mass as low as approximately 9 kDa in agarose gels. For higher resolution separation, and for extension to molecular masses as low as approximately 5 kDa, gradient polyacrylamide gels were superior. Densitometric scanning of stained gels allowed analysis of the range of molecular masses present in a sample, and calculation of weight-average and number-average values. The methods were validated for polydisperse HA samples with viscosity-average molecular masses of 112, 59, 37, and 22 kDa, at sample loads of 0.5 µg (for polyacrylamide) to 2.5 µg (for agarose). Use of the methods for electrophoretic mobility shift assays was demonstrated for binding of the HA-binding region of aggrecan (recombinant human aggrecan G1-IGD-G2 domains) to a 150 kDa HA standard.

Bhilocha, Shardul; Amin, Ripal; Pandya, Monika; Yuan, Han; Tank, Mihir; LoBello, Jaclyn; Shytuhina, Anastasia; Wang, Wenlan; Wisniewski, Hans-Georg; de la Motte, Carol; Cowman, Mary K.



Pressure Equilibrium and Jump Study on Unfolding of 23-kDa Protein from Spinach Photosystem II  

PubMed Central

Pressure-induced unfolding of 23-kDa protein from spinach photosystem II has been systematically investigated at various experimental conditions. Thermodynamic equilibrium studies indicate that the protein is very sensitive to pressure. At 20°C and pH 5.5, 23-kDa protein shows a reversible two-state unfolding transition under pressure with a midpoint near 160 MPa, which is much lower than most natural proteins studied to date. The free energy (?Gu) and volume change (?Vu) for the unfolding are 5.9 kcal/mol and ?160 ml/mol, respectively. It was found that NaCl and sucrose significantly stabilize the protein from unfolding and the stabilization is associated not only with an increase in ?Gu but also with a decrease in ?Vu. The pressure-jump studies of 23-kDa protein reveal a negative activation volume for unfolding (?66.2 ml/mol) and a positive activation volume for refolding (84.1 ml/mol), indicating that, in terms of system volume, the protein transition state lies between the folded and unfolded states. Examination of the temperature effect on the unfolding kinetics indicates that the thermal expansibility of the transition state and the unfolded state of 23-kDa protein are closer to each other and they are larger than that of the native state. The diverse pressure-refolding pathways of 23-kDa protein in some conditions were revealed in pressure-jump kinetics.

Tan, Cui-Yan; Xu, Chun-He; Wong, Jun; Shen, Jian-Ren; Sakuma, Shinsuke; Yamamoto, Yasusi; Lange, Reinhard; Balny, Claude; Ruan, Kang-Cheng



Evidence that the 59-kDa protein synthesis initiation factor from wheat germ is functionally similar to the 80-kDa initiation factor 4B from mammalian cells.  


The results of this investigation show that the 59-kDa protein synthesis initiation factor from wheat germ, designated eukaryotic initiation factor (eIF)-4G by Browning et al. (Browning, K.S., Maia, D.M., Lax, S.R., and Ravel, J.M. (1987) J. Biol. Chem. 262, 539-541), cross-links to the 5'-terminal cap of oxidized mRNA in the presence of eIF-4A, eIF-4F, and ATP, stimulates the RNA-dependent ATPase activities of eIF-4A and a mixture of eIF-4A and eIF-4F, and stimulates the unwinding activities of eIF-4A, eIF-4F, and a mixture of eIF-4A and eIF-4F. These findings strongly suggest that the 59-kDa factor from wheat germ is the functional equivalent of the 80-kDa protein synthesis initiation factor, eIF-4B, from mammalian cells. Recent reports indicate that the wheat germ initiation factor which contains two subunits of 80 and 28 kDa and which was given the designation "eIF-4B" by Lax et al. (Lax, S.R., Lauer, S.J., Browning, K. S., and Ravel, J.M. (1986) Methods Enzymol. 118, 109-128) is an isozyme form of eIF-4F and not the functional equivalent of mammalian eIF-4B. On the basis of functional characteristics we propose that the designation for the wheat germ factor containing the 80- and 28-kDa polypeptides be changed from eIF-4B to eIF-(iso)4F and the designation for the 59-kDa factor be changed from eIF-4G to eIF-4B. PMID:2722784

Browning, K S; Fletcher, L; Lax, S R; Ravel, J M



Project Da Vinci: a study of long-range air pollution using a balloon-borne Lagrangian measurement platform. Volume 2. Reports of participants in Da Vinci 1. Final report  

SciTech Connect

Understanding long-range air pollution is becoming increasingly important for the development of cost-effective air-quality control strategies. Project Da Vinci was the beginning of a continuing effort at Sandia to address the need to study long-range air pollution. It consisted of a series of three instrumented, manned balloon flights to study the lower atmosphere in a Lagrangian frame of reference. These experiments gathered information on gases and aerosols, radiation, electrical fields, atmospheric structure, turbulence, diffusion, mesoscale motions, and their interrelationship. Volume II is a collection of the reports from researchers involved in Da Vinci I.

Zak, B.D. (ed.)



The 20-Kilodalton (kDa) Human Growth Hormone (hGH) Differs from the 22-kDa hGH in the Effect on the Human Prolactin Receptor  

Microsoft Academic Search

Previously we have demonstrated that 20-kDa human GH (20K- hGH) is a full agonist for hGH receptor (hGHR) even though its complex formation with hGHR and hGH-binding protein differs from that of 22-kDa human GH (22K-hGH). In this study, we focused on the effect of 20K-hGH on human PRL receptor (hPRLR). To elucidate the effects of 20K-hGH on hPRLR and




Monoclonal antibody to the gastrin receptor on parietal cells recognizes a 78-kDa protein  

SciTech Connect

Four monoclonal antibodies reactive by immunofluorescence and by flow microfluorimetry with canine and porcine gastric parietal cell membranes were produced by fusion of mouse NS-1 myeloma cells with splenocytes from mice immunized with a population of canine gastric mucosal cells containing 60-70% parietal cells. One of these, an IgM antibody designated 2Cl, reacted with the surface membranes of parietal cells by immunofluorescence, flow microfluorimetry, and immunogold electron microscopy; competed with /sup 125/I-labeled gastrin for binding to gastric cells; and inhibited by 56% maximal gastrin stimulation of (/sup 14/C)aminopyrine uptake in parietal cells. The antibody immunoprecipitated /sup 125/I-labeled samples of a 78-kDa gastrin-binding protein purified from membrane extracts of porcine gastrin mucosa but did not recognize the same protein labeled covalently with /sup 125/I-labeled gastrin-(2-17)-hexadecapeptide. These observations suggest that the previously identified 78-kDa gastrin-binding protein is the gastrin receptor and that the antibody 2C1 is directed against the gastrin binding site of the gastrin receptor.

Mu, F.T.; Baldwin, G.; Weinstock, J.; Stockman, D.; Toh, B.H.



Observações espectroscópicas da candidata a pós-AGB IRAS 19386+0155  

NASA Astrophysics Data System (ADS)

Nesse trabalho apresentamos a análise fotosférica da estrela candidata a pós-AGB IRAS 19386+0155. Com os dados obtidos no espectrógrafo FEROS foram determinados os parâmetros atmosféricos e abundâncias fotosféricas utilizando o código MOOG. A análise do espectro mostrou que IRAS 19386+0155 possui os seguintes parâmetros atmosféricos : Teff = 6800K, log g = 1.4, [M/H] = -1.5 e Vt = 8.4 km/s. O padrão de abundância obtido para os elementos mais leves (Carbono, Nitrogênio e Oxigênio) e elementos a (Magnésio, Silício e Cálcio) foi inferior ao solar (log C = 7.74, log N = 7.28, Log O = 8.43, log Mg = 7.14, log Si = 7.54 e log Ca = 5.91). Uma inspeção visual do espectro ISO deste objeto revela a presença de poeira fria na forma de silicatos cristalinos. Embora as bandas mais marcantes de silicatos amorfos (em 10 mm e 18mm) não sejam observadas, a emissão em 21 mm, presente em algumas pós-AGBs também não está presente. O espectro ISO parece revelar um meio rico em oxigênio, mas a forma da distribuição de energia no infravermelho não obedece ao padrão apresentado por outras pós-AGBs. Nossos resultados nos levam a sugerir que IRAS 19386+0155 talvez faça parte de um sistema binário, uma vez que outras pós-AGBs que são membros de sistemas binários apresentam padrão de abundância semelhante.

Lorenz-Martins, S.; Pereira, C. B.



Establishing a Network of Next Generation SED Standards with DA White Dwarfs  

NASA Astrophysics Data System (ADS)

Photometric calibration systematic uncertainties are the dominant source of error in current type Ia supernova dark energy studies, as well as other forefront cosmology efforts, e.g. photo-redshift determinations for weak lensing mass tomography. Current and next-generation ground-based all-sky surveys require a network of calibration stars that 1) have known spectral energy distributions (SEDs) to properly and unambiguously account for differences in as-built filters, and 2) are on a common photometric zero-point scale. We exploit the well understood SEDs of DA white dwarf stars to establish a network of faint equatorial primary spectrophotometric standards. We present the measurements and analysis of 9 hot DA white dwarfs near 18th mag. The analysis of high S/N spectra from Gemini yields Teff and log(g), which in turn delivers the model SEDs from their pure hydrogen atmospheres. These model SEDs are compared against pan-chromatic photometry from HST WFC3 imaging to determine their reddening, and to establish their absolute flux scales without hindrance from the terrestrial atmosphere. This network of stars will directly calibrate and cross-validate the data products of current and future surveys. This precision photometric heritage from HST will benefit existing and upcoming survey projects (such as DES, Pan-STARRS and LSST), and directly addresses one of the current barriers to understanding the nature of dark energy.

Saha, Abhijit; Narayan, Gautham; Matheson, Thomas; Holberg, Jay B.; Stubbs, Christopher; Deustua, Susana E.; Bohlin, Ralph; Olszewski, Edward W.; Gilliland, Ronald L.; Axelrod, Tim S.; Rest, Armin



A Central X-ray Source in the Non-thermal Radio Nebula DA 495  

NASA Technical Reports Server (NTRS)

We report the detection, in archival ROSAT and ASCA observations, of X-ray emission from the direction of DA 495 (G65.7+1.2), a likely supernova remnant of uncertain classification but with similarities to the Crab Nebula. An unusual feature of the radio nebula is its annular morphology, with a flux minimum at the geometrical center. In the soft X-ray band, the ROSAT data resolve a compact source near the edge of the central radio hole ; the hard X-ray morphology, at the limit of ASCA's spatial resolution, is suggestive of extended plerionic emission dropping off from a central flux maximum coincident with the ROSAT source. The spectrum is well-described by a power-law with photon index approximately 1.7, and the X-ray flux is roughly constant with time. Taken together, this evidence suggests identification of the X-ray source with a magnetospherically active neutron star and its associated wind nebula. Timing analysis of the ASCA data yields only a weak upper bound on pulsations with periods longer than approximately 30 ms. These results reveal for the first time the high-energy engine that powers the synchrotron nebula, and strengthen the classification of DA 495 as a plerionic supernova remnant, one that may represent a late evolutionary stage of Crab-like nebulae.

Arzoumanian, Z.; Safi-Harb, S.; Landecker, T. L.; Kothes, R.



Purification of the 47 kDa phosphoprotein associated with the NADPH oxidase of human neutrophils.  

PubMed Central

A 47 kDa protein in the cytosol of phagocytic cells becomes heavily phosphorylated and translocates to the cell membrane upon stimulation. This protein was isolated from the cytosol of human neutrophils by chromatography on ion-exchange and hydroxyapatite resins. Polyclonal antibodies to this protein demonstrated that it was present in the neutrophils of two patients with X-linked chronic granulomatous disease (CGD) but not in those of three patients with the autosomal recessive pattern of inheritance. A sequence of amino acids was determined from a tryptic peptide of this protein: Glu-Met-Phe-Pro-Ile-Glu-Ala-Gly-Ala-Ile-Asn-Xaa-Glu. This served to establish that the phosphoprotein isolated here is the same as a protein of a similar molecular mass identified by other workers. These studies confirm the involvement of this 47 kDa phosphoprotein in the molecular pathology of autosomal recessive CGD and describe a method for the purification of the native protein. Images Fig. 1. Fig. 2. Fig. 3.

Teahan, C G; Totty, N; Casimir, C M; Segal, A W



Discovery of Photospheric Helium in the Ultra-Massive DA White Dwarf GD 50  

NASA Astrophysics Data System (ADS)

Extreme-ultraviolet (EUV) spectroscopy of the hot, hydrogen-rich white dwarf GD 50 reveals an unusual photospheric mixture of hydrogen and helium. This hot DA white dwarf is also remarkable for its mass (~1.2 M?) near the Chandrasekhar limit. The spectra obtained with the Extreme-Ultraviolet Explorer (EUVE) show a prominent He II resonance line series and constrain its atmospheric parameters to Teff = 40,300 +/- 100 K and n(He)/n(H) = (2.4 +/- 0.1) x 10-4 (assuming log g = 9.0). We also constrain the local interstellar medium column densities of neutral helium and hydrogen to nHe I = 6 x 1016 and nH I = 9 x 1017 cm-2. The presence of helium in an isolated, massive DA white dwarf is paradoxical. We examine and rule out a couple of scenarios (radiative levitation, accretion from the ISM or a hypothetical companion), but we suggest that if massive white dwarfs do result from stellar mergers, large orbital angular momentum may be preserved and result in a large meridional circulation current, possibly dredging up helium from the envelope. Although we find evidence of large rotational velocity in EUV He II line profiles, we propose additional observational tests of the dredge-up model.

Vennes, Stephane; Bowyer, Stuart; Dupuis, Jean



Development of a benthic multimetric index for the Serra da Bocaina bioregion in Southeast Brazil.  


Brazil faces a challenge to develop biomonitoring tools to be used in water quality assessment programs, but few multimetric indices were developed so far. This study is part of an effort to test and implement programs using benthic macroinvertebrates as bioindicators in Rio de Janeiro State. Our aim was first to test the Multimetric Index for Serra dos Órgãos (SOMI) for a different area--Serra da Bocaina (SB)--in the same ecoregion. We sampled 27 streams of different sizes and altitudes in the SB region. Despite the environmental similarities, results indicated biological differences between reference sites of the two regions. Considering these differences, we decided to develop an index specific for the SB region, the Serra da Bocaina Multimetric Index (MISB). We tested twenty-two metrics for sensitivity to impairment and redundancy, and six metrics were considered valid to integrate the MISB: Family Richness, Trichoptera Richness, % Coleoptera, % Diptera, IBE-IOC index, EPT / Chironomidae ratio. A test of the MISB in eleven sites indicated it was more related to land-use and water physico-chemical parameters than with altitude or stream width, being a useful tool for the monitoring and assessment of streams in the bioregion. PMID:24212698

Baptista, D F; Henriques-Oliveira, A L; Oliveira, R B S; Mugnai, R; Nessimian, J L; Buss, D F



Characterization of a 19 kDa alpha-zein of high purity.  


A highly pure alpha-zein was extracted from corn flour using ethanol (95%). Subsequently, ion-exchange chromatography was performed, using SP-Sepharose that yielded a highly homogeneous protein. This protein migrated as a single band in 20% SDS-PAGE and in pH gradient gels, showing an isoelectric point of 6.8. Mass spectrometry (MALDI-TOF-MS) showed a single peak with a molecular mass of 24 535 Da. It was identified as Z19, when comparing the sequence obtained in an automatic Edman sequencer with the Swissprot database using BLAST. The molar extinction coefficient, determined by dry weight in 70% methanol, was 12 415.49 M(-1) cm(-1) at 280 nm. Light scattering showed its presence in a monodispersed state of 44-66 kDa aggregates in methanol (70%). Circular dichroism spectra allowed the estimation of an alpha-helix content that was lower than the one found for a mixture of two alpha-zeins but with a higher content of beta sheets. PMID:15686426

Cabra, Vanessa; Arreguin, Roberto; Galvez, Amanda; Quirasco, Maricarmen; Vazquez-Duhalt, Rafael; Farres, Amelia



Crystal Structure of the 25 kDa Subunit of Human Cleavage Factor I{m}  

SciTech Connect

Cleavage factor Im is an essential component of the pre-messenger RNA 3'-end processing machinery in higher eukaryotes, participating in both the polyadenylation and cleavage steps. Cleavage factor Im is an oligomer composed of a small 25 kDa subunit (CF Im25) and a variable larger subunit of either 59, 68 or 72 kDa. The small subunit also interacts with RNA, poly(A) polymerase, and the nuclear poly(A)-binding protein. These protein-protein interactions are thought to be facilitated by the Nudix domain of CF Im25, a hydrolase motif with a characteristic {alpha}/{beta}/{alpha} fold and a conserved catalytic sequence or Nudix box. We present here the crystal structures of human CF Im25 in its free and diadenosine tetraphosphate (Ap4A) bound forms at 1.85 and 1.80 Angstroms, respectively. CF Im25 crystallizes as a dimer and presents the classical Nudix fold. Results from crystallographic and biochemical experiments suggest that CF Im25 makes use of its Nudix fold to bind but not hydrolyze ATP and Ap4A. The complex and apo protein structures provide insight into the active oligomeric state of CF Im and suggest a possible role of nucleotide binding in either the polyadenylation and/or cleavage steps of pre-messenger RNA 3'-end processing.

Coseno,M.; Martin, G.; Berger, C.; Gilmartin, G.; Keller, W.; Doublie, S.



ProDaMa: an open source Python library to generate protein structure datasets  

PubMed Central

Background The huge difference between the number of known sequences and known tertiary structures has justified the use of automated methods for protein analysis. Although a general methodology to solve these problems has not been yet devised, researchers are engaged in developing more accurate techniques and algorithms whose training plays a relevant role in determining their performance. From this perspective, particular importance is given to the training data used in experiments, and researchers are often engaged in the generation of specialized datasets that meet their requirements. Findings To facilitate the task of generating specialized datasets we devised and implemented ProDaMa, an open source Python library than provides classes for retrieving, organizing, updating, analyzing, and filtering protein data. Conclusion ProDaMa has been used to generate specialized datasets useful for secondary structure prediction and to develop a collaborative web application aimed at generating and sharing protein structure datasets. The library, the related database, and the documentation are freely available at the URL .

Armano, Giuliano; Manconi, Andrea



The remarkably low abundance of helium in the atmosphere of the DA white dwarf HZ43  

NASA Astrophysics Data System (ADS)

We report the most sensitive search yet made for helium in the atmosphere of the hot DA white dwarf HZ43, with observations made by the Extreme Ultraviolet Explorer (EUVE). Prior upper limits on the presence of He in DA white dwarfs using EUVE spectra have been affected by fixed-pattern efficiency variations in the detector system, which limited the signal-to-noise ratio that could be achieved. Recent implementation of a new observing mode designed to circumvent this problem has yielded an approximately fourfold improvement in the effective signal-to-noise ratio. This allows detection of weaker absorption features than was hitherto possible and, in the absence of visible lines, provides dramatically improved upper limits. We find that the He abundance (by number fraction) in HZ43 is less than ~=3x10^-7. This is completely at odds with the calculations of radiative levitation theory, which predict a He abundance of at least 3x10^-6 for a star with the mass and temperature of HZ43. This has important consequences for our understanding of the physical mechanisms that determine the photospheric composition of white dwarfs.

Barstow, M. A.; Holberg, J. B.; Koester, D.



Insights into the morphology of the Serra da Cangalha impact structure from geophysical modeling  

NASA Astrophysics Data System (ADS)

Forward modeling is commonly applied to gravity field data of impact structures to determine the main gravity anomaly sources. In this context, we have developed 2.5-D gravity models of the Serra da Cangalha impact structure for the purpose of investigating geological bodies/structures underneath the crater. Interpretation of the models was supported by ground magnetic data acquired along profiles, as well as by high resolution aeromagnetic data. Ground magnetic data reveal the presence of short-wavelength anomalies probably related to shallow magnetic sources that could have been emplaced during the cratering process. Aeromagnetic data show that the basement underneath the crater occurs at an average depth of about 1.9 km, whereas in the region beneath the central uplift it is raised to 0.5-1 km below the current surface. These depths are also supported by 2.5-D gravity models showing a gentle relief for the basement beneath the central uplift area. Geophysical data were used to provide further constraints for numeral modeling of crater formation that provided important information on the structural modification that affected the rocks underneath the crater, as well as on shock-induced modifications of target rocks. The results showed that the morphology is consistent with the current observations of the crater and that Serra da Cangalha was formed by a meteorite of approximately 1.4 km diameter striking at 12 km s-1.

Vasconcelos, M. A. R.; Wünnemann, K.; Crósta, A. P.; Molina, E. C.; Reimold, W. U.; Yokoyama, E.



Membrane-associated 41-kDa GTP-binding protein in collagen-induced platelet activation  

SciTech Connect

Initially we established that the binding of collagen to human blood platelets stimulates both the rapid loss of PIP2 and the generation of inositol-4,5-bisphosphate (IP2) and inositol-1,4,5-triphosphate (IP3). These results indicate that the binding of collagen stimulates inositol phospholipid-specific phospholipase C during platelet activation. The fact that GTP or GTP-gamma-S augments, and pertussis toxin inhibits, collagen-induced IP3 formation suggests that a GTP-binding protein or (or proteins) may be directly involved in the regulation of phospholipase C-mediated phosphoinositide turnover in human platelets. We have used several complementary techniques to isolate and characterize a platelet 41-kDa polypeptide (or polypeptides) that has a number of structural and functional similarities to the regulatory alpha i subunit of the GTP-binding proteins isolated from bovine brain. This 41-kDa polypeptide (or polypeptides) is found to be closely associated with at least four membrane glycoproteins (e.g., gp180, gp110, gp95, and gp75) in a 330-kDa complex that can be dissociated by treatment with high salt plus urea. Most important, we have demonstrated that antilymphoma 41-kDa (alpha i subunit of GTP-binding proteins) antibody cross-reacts with the platelet 41-kDa protein (or proteins) and the alpha i subunit of bovine brain Gi alpha proteins, and blocks GTP/collagen-induced IP3 formation. These data provide strong evidence that the 41-kDa platelet GTP-binding protein (or proteins) is directly involved in collagen-induced signal transduction during platelet activation.

Walker, G.; Bourguignon, L.Y. (Univ. of Miami Medical School, FL (USA))



L151-81A/B - A unique white dwarf binary with DB and DA components  

NASA Technical Reports Server (NTRS)

Spectroscopic observations of the wide binary L151-81A/B reveal that both components are white dwarfs with spectral types DB3 and DA4, the first such binary identified in a comprehensive survey of Luyten and Giclas common proper motion pairs. Assuming log g = 8, measurements of the helium line profiles in the DB primary yield Teff = 16,000 + or - 2,000, while the hydrogen profiles for the DA companion yield Teff =12,000 + or - 2,000. The existence of a helium-rich/hydrogen-rich degenerate pair offers several interesting tests of theoretical white dwarf formation channels and surface abundance evolution.

Oswalt, Terry D.; Hintzen, Paul M.; Liebert, James W.; Sion, Edward M.



Effect of DA-9701 on gastric emptying in a mouse model: Assessment by 13C-octanoic acid breath test  

PubMed Central

AIM: To evaluate the effects of DA-9701 on the gastric emptying of a solid meal using the 13C-octanoic acid breath test in a mouse model. METHODS: Male C57BL/6 mice aged > 8 wk and with body weights of 20-25 g were used in this study. The solid test meal consisted of 200 mg of egg yolk labeled with 1.5 L/g 13C-octanoic acid. The mice were placed in a 130 mL chamber flushed with air at a flow speed of 200 mL/min. Breath samples were collected for 6 h. The half-emptying time and lag phase were calculated using a modified power exponential model. To assess the reproducibility of the 13C-octanoic acid breath test, the breath test was performed two times at intervals of one week in ten mice without drug treatment. To assess the gastrokinetic effects of DA-9701, the breath test was performed three times in another twelve mice, with a randomized crossover sequence of three drug treatments: DA-9701 3 mg/kg, erythromycin 6 mg/kg, or saline. Each breath test was performed at an interval of one week. RESULTS: Repeatedly measured half gastric emptying time of ten mice without drug treatment showed 0.856 of the intraclass correlation coefficient for the half gastric emptying time (P = 0.004). The mean cumulative excretion curve for the 13C-octanoic acid breath test showed accelerated gastric emptying after DA-9701 treatment compared with the saline control (P = 0.028). The median half gastric emptying time after the DA-9701 treatment was significantly shorter than after the saline treatment [122.4 min (109.0-137.9 min) vs 134.5 min (128.4-167.0 min), respectively; P = 0.028] and similar to that after the erythromycin treatment [123.3 min (112.9-138.2 min)]. The lag phase, which was defined as the period taken to empty 15% of a meal, was significantly shorter after the DA-9701 treatment than after the saline treatment [48.1 min (44.6-57.1 min) vs 52.6 min (49.45-57.4 min), respectively; P = 0.049]. CONCLUSION: The novel prokinetic agent DA-9701 accelerated gastric emptying, assessed with repeated measurements in the same mouse using the 13C-octanoic acid breath test. Our findings suggest that DA-9701 has therapeutic potential for the treatment of functional dyspepsia.

Lim, Chul-Hyun; Choi, Myung-Gyu; Park, Hyeyeon; Baeg, Myong Ki; Park, Jae Myung



Efeitos da Exploração Madeireira de Baixo Impacto sobre uma Comunidade de Aves de Sub-bosque na Floresta Nacional do Tapajós, Pará, Brasil  

Microsoft Academic Search

RESUMO Este estudo compara a resposta de uma comunidade aves à exploração madeireira de baixo impacto através da distribuição das espécies em floresta controle e manejada ao longo de cinco anos com amostragem pré e pós-exploração. O procedimento de ordenação mostrou que a similaridade da comunidade de aves após a exploração madeireira foi menor que a similaridade entre as amostras

Luiza Magalli; Pinto HENRIQUES; Joseph M. WUNdERLE; C. OREN; Michael R. WILLIG


ESTUDO DA LIGAÇÃO CRUZADA INDUZIDA PELO FORMALDEÍDO EM CÁPSULAS DE GELATINA DURA Study of formaldehyde induced cross-linking in hard gelatin capsules  

Microsoft Academic Search

RESUMO: O formaldeído é um agente desnaturante que induz a ligação cruzada covalente na gelatina, sendo um dos agentes utilizados para a promoção da gastro-resistência em formas farmacêuticas gelatinosas, contudo, seu uso carece de avaliações minuciosas e da definição de protocolos específicos para as diversas classes de fármacos. O objetivo deste trabalho foi investigar a ligação cruzada induzida pelo formaldeído

Ana Lúcia; T. C. Zampieri; Eliana M. Lima; Danielle G. A. Diniz


Variação da independência funcional em idosos hospitalizados relacionada a variáveis sociais e de saúde Variation in functional independence in hospitalized elderly related to social and health variables  

Microsoft Academic Search

O processo de envelhecimento, as doenças crônicas não transmissíveis e as hospitalizações podem causar declínio funcional em idosos. Alguns fatores podem potencializar esse comprometimento funcional como gênero, número de internações, presença de acompanhante e medicações em uso. Objetivo: Identificar a variação da capacidade funcional em idosos no decorrer da hospitalização e relacionar a diferença com variáveis sociais e de saúde.

Kozue Kawasaki; Maria José D'Elboux Diogo




Microsoft Academic Search

Face à importância da informação para o atual contexto social, em especial para a gestão empresarial, analisa-se a informação como um ativo a ser gerenciado, uma vez que é infinitamente reutilizável, não deteriorável e nem tampouco depreciável. A gestão da informação transforma valores e cultura organizacionais. Sua efetivação contribui para o alcance dos atuais desafios das empresas: qualidade, produtividade e

Andreina Alves de Sousa


USO DA CULTURA DE CÉLULAS EM TESTES DIAGNÓSTICOS LABORATORIAIS EM MEDICINA E BIOLOGIA The use of cell culture in laboratory diagnostic tests in medicine and biology  

Microsoft Academic Search

RESUMO A cultura de células, iniciada nos primórdios do século XIX, vem sendo cada vez mais adaptada à manutenção do metabolismo fundamental celular in vitro, permitindo o desenvolvimento da maioria das funções fisiológicas básicas inerentes a uma célula e ao conjunto delas (tecido). A permanência da informação genética trazida do organismo que lhe deu origem, faz com que as células

Iracina Maura de Jesus; Maria Izabel de Jesus; Walber Victor de Moraes Pinto; Renato Lopes Fernandes Medeiros; Dorotéa de Fátima; Lobato da Silva


Migration from can coatings: part 2. Identification and quantification of migrating cyclic oligoesters below 1000 Da.  


Metal cans for food use can be coated with lacquers based on polyester resins. Recent research has focussed on the identification and quantification of migrants released by coatings that are potentially absorbable (below 1000 Da). The presented method describes a procedure that was optimized to hydrolyse the polyester migrants into their monomers, polyvalent acids and polyols. The polyols were identified by gas chromatography with flame ionization detection GC-FID and the acids by high-performance liquid chromatography (HPLC) coupled with an ultraviolet and an electrospray ionization-mass selective detector (HPLC-ESI-MSD/UVD), respectively. With the knowledge of the polyester monomers, it was possible--at least tentatively--to identify the main components in the migrate as cyclic oligoesters by HPLC-ESI-MSD/UVD. A cyclic oligomer, CYCLO [3IPA (isophthalic acid) 3EG (ethylene glycol)] was synthesized and characterized by infrared, nuclear magnetic resonance and mass spectrometry as well as by elementary analysis for further confirmation. To determine the amount of migrating cyclic oligoesters, the response of the migrating substances was compared using different detectors, UVD, MSD and evaporative light scattering detector (ELSD). The response of the ELSD was dependent on the molecular weight of the analytes that reduced the accuracy of this detection type. The wavelength with the same absorption coefficient for IPA and terephthalic acid (TPA) was obtained at 232 nm. The UV(232nm) response of an oligoester is proportional to the number of its IPA/TPA moieties, which was verified for several TPA/IPA esters. The amount of the migrating oligoesters was determined using an UV(232nm) calibration of a commercially available TPA ester and the number of IPA/TPA moieties molecules gained from the ESI-MSD spectra. According to this method, the amount of migrating oligoesters below 1000 Da in the 95% ethanol migrate varied from 0.1 to 0.6 mg dm(-2) (0.6-3.6 mg kg(-1) food) in the examined coatings. The determined amounts account for about 50% of the total migrate below 1000 Da. PMID:15204562

Schaefer, A; Ohm, V A; Simat, T J



Micromorphology of selected relict slope deposits from Serra da Estrela, Portugal  

NASA Astrophysics Data System (ADS)

Serra da Estrela is the highest mountain in Portugal (1,993 m ASL) and part of the Iberian Central Cordillera. The mountain has a strong relief and a lithological diversity with several types of granitoids and metasediments. Most of the western plateau area was glaciated during the Last Glacial Maximum and its morphology is dominated by glacial landforms. Vieira (2004) produced a detailed geomorphological map of Serra da Estrela and described several sites showing stratified slope, head and debris-flow deposits. Based on the geomorphological analysis of the relationships between glacial and periglacial evidence, a first relative chronology was presented. However, a detailed and systematical sedimentological analysis has not been conducted before and absolute ages are also lacking. Micromorphology analysis has proven to be of considerable value in the interpretation of mountain soils and sediments. Such interpretation depends on identifying diagnostic features, indicating factors as the presence or absence of permafrost, thickness of the active layer, ice segregation and the operation of processes of mass-wasting. In this study, micromorphology was used to answer questions concerning the composition, structure, origin and depositional processes of relict slope deposits. Micromorphology allowed a systematic description of the physical characteristics of the sediments. Lamination and sorting, when preserved, are good evidence for overland flow. Features due to deformation (folds, boudins, coatings and tails due to the rotation of clasts) are associated with sliding. Other mass-movements such as debris flows, earth flows, and to a certain extent, dry grain flows may be characterized by similar microscopic facies, typically a poorly sorted, porphyric material. Porosity gives evidence for both liquefaction (debris flows) and frost-induced mass-movement (solifluction). The relict slope deposits of the Serra da Estrela show an increase in cryogenic micromorphological features with altitude. Above 1100-1200 m, solifluction microstructures become frequent and very well-developed, evidencing frost-induced mass movement. Below this altitude solifluction processes were less frequently observed, and with poorly developed microfeatures. Debris-flow and run-off processes occur in a wider altitudinal range and are controlled by local topographical and geomorphological conditions.

Nieuwendam, Alexandre; Vieira, Gonçalo; Schaefer, Carlos



Avaliação sazonal da atividade da fenilalanina amônia-liase e dos teores de fenóis e taninos totais em Byrsonima verbascifolia Rich. ex A. Juss.: uma espécie medicinal do cerrado  

Microsoft Academic Search

RESUMO: Com o objetivo de investigar a influência de variações sazonais do ambiente físico, no estado hídrico foliar, nas trocas gasosas, na atividade da fenilalanina amônia-liase e nos teores de fenóis e taninos totais em murici, um experimento foi conduzido, a uma freqüência mensal, em área de formação campestre, com fisionomia de Cerrado sensu strictu, localizada no município de Ijaci,

A. M. Soares; R. Padre José Poggel


In para totale...una cosa da panico...sulla lingua dei giovani in Italia (In para totale...una cosa da panico...The Language of Young People in Italy).  

ERIC Educational Resources Information Center

Describes and analyzes the language of young people in Italy today. Particular focus is on the expressions using "para" (e.g., "in para totale" = to be very bored or worried) and the phrase "una cosa da panico" (something terrible or its opposite something wonderful). (CFM)

Marcato, Carla



Chlorophyll antenna proteins of photosystem I: topology, synthesis, and regulation of the 20-kDa subunit of Chlamydomonas light-harvesting complex of photosystem I  

SciTech Connect

The light-harvesting complex of photosystem I (LHCI) was isolated from wild-type cells of Chlamydomonas reinhardtii; the Chl a/b-protein complex contains four major polypeptides of approximately 27, 26, 24, and 20 kDa (polypeptides 14, 15, 17.2, and 22, respectively, in the nomenclature for Chlamydomonas thylakoid proteins). Antiserum against the 20-kDa subunit of LHCI was prepared and used to determine the membrane topology, subcellular site of synthesis, and cell-cycle regulation of this polypeptide. The results indicate that the 20-kDa subunit as well as the other major LHCI polypeptides are integral membrane proteins. Moreover, protease digestion experiments reveal that the 20-kDa polypeptide is completely protected by the membrane bilayer but the 27- and 26-kDa LHCI polypeptides are exposed at the membrane surface. In vivo synthesis of the 20-kDa polypeptide is sensitive to cycloheximide but not to chloramphenicol; the form of the polypeptide recovered from in vitro translations of polyadenylated RNA is approximately 24 kDa, 4 kDa larger than the mature polypeptide. It is concluded that this LHCI polypeptide is nuclear encoded and synthesized in the cytoplasm as a higher molecular weight precursor. Synthesis of the 20-kDa polypeptide is restricted to the light period in light-dark synchronized cells. Translatable mRNA for this polypeptide accumulates during the light but levels are dramatically reduced during the dark period. Thus, synthesis of the 20-kDa subunit of LHCI appears to be transcriptionally regulated during the cell cycle.

Herrin, D.L.; Plumley, F.G.; Ikeuchi, M.; Michaels, A.S.; Schmidt, G.W.



Regulation of heat shock protein 72 kDa and 90 kDa in human breast cancer MDA-MB-231 cells.  


It has been shown that expression of HSPs can negatively regulate the effectiveness of cytotoxic drugs. In this study, we conducted experiments to study the regulation of expression of heat shock proteins (HSPs) in human breast cancer MDA-MB-231 cells. Using [35S]methionine incorporation and Western immunoblots, we established that heat shock increased production of HSP-72 and -90. Cells exposed to 44 degrees C for 20 min displayed increased expression of HSP-72 and -90, that reached a maximum 3-7 h later and returned to baseline levels within 24 h. The synthesis of both HSP-72 and -90 was attenuated when cells were exposed to heat shock in medium devoid of Ca2+ or pretreated with the calcium chelator BAPTA for 30 min prior to heat shock. Similarly, synthesis of HSP-72 and -90 was inhibited when cells were treated with the protein kinase A inhibitor, H89. These data indicate that Ca2+ and PKA are involved in the regulation of HSP-72 and -90 protein synthesis. Levels of HSP-72 mRNA in cells exposed to heat shock increased, suggesting that the heat-induced increase in HSP-72 occurs at the transcriptional level. Also, heat shock caused phosphorylation and translocation from the cytosol to the nucleus of heat shock factor 1 (HSF 1), a transcription factor for heat shock protein synthesis. Removal of external Ca2+ or treatment with a PKA inhibitor prevented the phosphorylation and the translocation of HSF 1. Cells overexpressing HSP-72 and -90 induced by exposure to a sublethal temperature displayed cytoprotection from thermal injury. Removal of external Ca2+ and treatment with BAPTA or H89 prior to exposure to sublethal heat shock that reduced the amount of HSP-72 and -90 production still protected cells from subsequent thermal injury. The intracellular free calcium concentration ([Ca2+]i) in resting fura-2-loaded MDA-MB-231 cells was 175+/- nM. Heat shock increased [Ca2+]i in a time-and temperature-dependent manner. Exposure of cells to 44 degrees C for 20 min increased [Ca2+]i by 234+/-13%, which subsequently returned to baseline levels within 30 min. Removal of external Ca2+ eliminated the increase, indicating that the increase in [Ca2+]i was due to Ca2+ influx. Pretreatment of the cells with H89 but not GF-109203X for 30 min led to an attenuation of the increase in [Ca2+]i by a subsequent heat shock. The results suggest that HSP-72 and -90 are regulated by [Ca2+]i and PKA activity in MDA-MB-231 cells. Kiang JG, Gist ID, Tsokos GC: Regulation of Heat Shock Protein 72 kDa and 90 kDa in Human Breast Cancer MDA-MB-231 Cells. PMID:10718636

Kiang, J G; Gist, I D; Tsokos, G C



Theoretical profiles of Lyman-alpha satellites and application to synthetic spectra of DA white dwarfs  

NASA Technical Reports Server (NTRS)

We present new theoretical calculations for the red wing of the Lyman-alpha profile. Close collisions with neutral and ionized hydrogen lead to the formation of the pseudomolecules H-H and H-H(+) with the appearance of satellite features near 1600 and 1400 A. The calculations include multiperturber effects, which are responsible for the formation of H3 and H3 with features near 1950 and 2600 A. The theoretical absorption profiles are included in stellar atmosphere codes and used to predict synthetic spectra for DA white dwarfs of intermediate temperatures (20,000 to 8000 K). These new calculations offer a unique opportunity to determine accurate effective temperatures and surface gravities for the variable ZZ Ceti stars.

Allard, N. F.; Koester, D.



Sequences of two porcine glutamic acid decarboxylases (65- and 67-kDa GAD).  


The cDNAs encoding the porcine 65- and 67-kDa glutamic acid decarboxylases (GAD65 and GAD67, respectively) were cloned by the PCR method. The 2246-nucleotide (nt) GAD65 cDNA contained an open reading frame (ORF) coding for a protein of 585 amino acids (aa), and the 3262-nt GAD67 cDNA contained an ORF coding for a protein of 594 aa. A remarkable conservation was shown when the deduced aa sequences of porcine GAD65 and GAD67 were compared with those of other mammalian species (human, cat and rat). Porcine GAD65 is 96% identical to human and rat GAD65, and porcine GAD67 is more than 95% identical to human, cat and rat GAD67 at the aa level. PMID:7835711

Suzuki, R; Asami, N; Amann, E; Wagatsuma, M




SciTech Connect

We report the most rapid rate of period change measured to date for a pulsating DA (hydrogen atmosphere) white dwarf (WD), observed in the 292.9 s mode of WD 0111+0018. The observed period change, faster than 10{sup -12} s s{sup -1}, exceeds by more than two orders of magnitude the expected rate from cooling alone for this class of slow and simply evolving pulsating WDs. This result indicates the presence of an additional timescale for period evolution in these pulsating objects. We also measure the rates of period change of nonlinear combination frequencies and show that they share the evolutionary characteristics of their parent modes, confirming that these combination frequencies are not independent modes but rather artifacts of some nonlinear distortion in the outer layers of the star.

Hermes, J. J.; Montgomery, M. H.; Winget, D. E. [Department of Astronomy, University of Texas at Austin, Austin, TX - 78712 (United States)] [Department of Astronomy, University of Texas at Austin, Austin, TX - 78712 (United States); Mullally, Fergal [SETI Institute, 189 Bernardo Avenue, Suite 100, Mountain View, CA 94043 (United States)] [SETI Institute, 189 Bernardo Avenue, Suite 100, Mountain View, CA 94043 (United States); Bischoff-Kim, A., E-mail: [Chemistry, Physics and Astronomy Department, Georgia College and State University, Milledgeville, GA 31061 (United States)



Single-photon ionization of organic molecules beyond 10 kDa.  


The volatilization and soft ionization of complex neutral macromolecules at low energies has remained an outstanding challenge for several decades. Most volatilization techniques in mass spectrometry produce ions already in the source and most of them lead to particle velocities in excess of several hundred meters per second. For many macromolecules, post-ionization is inefficient since electronic or optical excitations can be followed by competing non-ionizing internal conversion, electron recapture, or fragmentation processes. Here, we explore the laser-assisted volatilization of neutral perfluoroalkyl-functionalized tetraphenylporphyrins as well as their single-photon ionization using vacuum ultraviolet (VUV) light at 157 nm. A systematic investigation of the ionization curves allows us to determine the molecular velocity distribution and ionization cross sections. We demonstrate the detection of single photon ionized intact organic molecules in excess of 10 kDa from a slow molecular beam. PMID:23444050

Schmid, Philipp; Stöhr, Frederik; Arndt, Markus; Tüxen, Jens; Mayor, Marcel



Translocator Protein (18 kDa): A Promising Therapeutic Target and Diagnostic Tool for Cardiovascular Diseases  

PubMed Central

The translocator protein (18?kDa) (TSPO) is a five transmembrane domain protein in mitochondria, abundantly expressed in a variety of organs and tissues. TSPO contributes to a wide range of biological processes, including cholesterol transportation, mitochondrial membrane potential and respiratory chain regulation, apoptosis, and oxidative stress. Recent studies have demonstrated that TSPO might also be involved in the physiological regulation of cardiac chronotropy and inotropy. Accordingly, TSPO ligands play significant roles in protecting the cardiovascular systems under pathological conditions through cardiac electrical activity retention, intracellular calcium maintenance, mitochondrial energy provision, mitochondrial membrane potential equilibrium, and reactive oxygen species inhibition. This paper focuses on the physiological and pathological characteristics of TSPO in the cardiovascular systems and also summarizes the properties of TSPO ligands. TSPO represents a potential therapeutic target and diagnostic tool for cardiovascular diseases including arrhythmia, myocardial infarction, cardiac hypertrophy, atherosclerosis, myocarditis, and large vessel vasculitis.

Qi, Xiaolong; Xu, Jiahong; Wang, Fei; Xiao, Junjie



Electronic structure calculations for the study of D-?-A organic sensitizers: Exploring polythiophene linkers  

NASA Astrophysics Data System (ADS)

In this work we present a detailed study of the atomic and electronic structure of a collection of push-pull organic dyes for high-performance sensitized solar cells (DSSCs). We compare the computed photophysical properties of donor-bridge-acceptor (D-?-A) dyes with polythiophene (Tn) or polycyclopentadithiophene (Cn) conjugated linkers with up to four fused thiophene rings. Excitation energies to lowest excited singlet state have been rationalized by means of fragment and molecular orbitals. Vertical and adiabatic excitation energies are systematically lower for the Cn family and become smaller with the length of the molecular conjugation. We discuss a large variety of computational techniques for the characterization of the charge transfer (CT) nature of the electronic excitation. In addition to standard procedures to quantify CT character, we propose and explain several novel interaction based measures of CT. Finally, we have computed ground and excited state oxidation potentials (GSOP and ESOP) with long-range corrected (LRC) functional.

Climent, Clàudia; Casanova, David



[Teaching, research, and extension service: botanist Honório da Costa Monteiro Filho].  


The article revisits the work of Honório da Costa Monteiro Filho, highlighting his contribution to the study of economic botany and the taxonomy of Brazilian Malvaceae. Many of his seventy articles, are still cited. Yet little is known about his important role in educating agronomists involved with Brazilian flora and the creation of the Botanical Society of Brazil. These topics are discussed in the article, along with his work on a project to reform the teaching of agronomy in Brazil. The entire works of Monteiro Filho, archival documents, his correspondence with other scientists, and his observations on plant labels in herbaria were researched; interviews were also conducted with people with ties to him. PMID:23295656

Bovini, Massimo G; Peixoto, Ariane Luna



Interaction between Theileria orientalis 23-kDa piroplasm membrane protein and heparin.  


The 23-kDa piroplasm membrane protein of Theileria orientalis (p23) is an immunogenic protein expressed during the intraerythrocytic stage of the parasite; its function, however, remains unclear. To evaluate the host factor or factors that interact with p23, we examined the binding of p23 to components of the host cell surface. Recombinant p23 protein of the Ikeda genotype failed to bind to bovine red blood cells or to peripheral blood mononuclear cells, but did bind to Madin-Darby Bovine Kidney (MDBK) cells. A glycoarray assay showed that recombinant p23 proteins from the three genotypes bound to heparin, indicating that p23 is a heparin-binding Theileria surface molecule. Further analysis of heparin-binding molecules is useful for understanding attachment and invasion of T. orientalis merozoites. PMID:24979990

Takemae, Hitoshi; Sugi, Tatsuki; Kobayashi, Kyousuke; Murakoshi, Fumi; Recuenco, Frances C; Ishiwa, Akiko; Inomata, Atsuko; Horimoto, Taisuke; Yokoyama, Naoaki; Kato, Kentaro; Kato, Kentaro



First Results of the VLBI Experiment on Radar Location of the Asteroid 2012 DA14  

NASA Astrophysics Data System (ADS)

An international VLBI experiment on radio location of the asteroid 2012 DA14 was organized on 2013 February 15--16, during its flyby close to Earth. The purpose of observations was to investigate and specify orbital parameters of the asteroid, as well as to evaluate its rotation period and other characteristics. The irradiation of the asteroid was performed by the RT-70 transmitter at Evpatoria (Crimea, Ukraine), while the reflected signals were successfully accepted by the two 32 m radio telescopes at Medicina (Bologna, Italy) and Irbene (Ventspils, Latvia). Processing and interpretation of the data were performed both in the Radiophysical Research Institute and in the Ventspils International Radio Astronomy Center. The first results of this experiment are presented and discussed.

Nechaeva, M.; Antipenko, A.; Bezrukov, D.; Bezrukovs, Vl.; Dementjev, A.; Dugin, N.; Jekabsons, N.; Khutornoy, R.; Klapers, M.; Konovalenko, A.; Kulishenko, V.; Nabatov, A.; Nesteruk, V.; Pupillo, G.; Reznichenko, A.; Salerno, E.; Shmeld, I.; Skirmante, K.; Tikhomirov, Yu.; Voytyuk, V.


Test of large area glass RPCs at the DA?NE Test Beam Facility (BTF)  

NASA Astrophysics Data System (ADS)

The CaPiRe program has been started to develop a new detector design, in order to produce large areas of glass Resistive Plate Chambers (RPC) detectors, overcoming the previous limitations. As a first step we produced our glass RPC detectors (1 m2) at General Tecnica exploiting their standard procedures, materials and production techniques simply using 2 mm glass electrodes instead of the bakelite ones. A set of RPC was produced by using pre-coated (silk screen printed) electrodes, while others were produced with the standard graphite coating. All the detectors, together with four old Glass RPC acting as reference, were tested at the DA?NE Test Beam Facility with 500 MeV electrons in order to study the efficiency in different positions inside the detectors (i.e. near spacers and edges) and to study the detector behavior as a function of the local particle rate.

Calcaterra, A.; de Sangro, R.; Gamba, D.; Mannocchi, G.; Patteri, P.; Picchi, P.; Piccolo, M.; Ragazzi, S.; Redaelli, N.; Satta, L.; Tabarelli de Fatis, T.; Tonazzo, A.; Trapani, P.; Trinchero, G. C.



Asteroid 1950 DA's encounter with Earth in 2880: physical limits of collision probability prediction.  


Integration of the orbit of asteroid (29075) 1950 DA, which is based on radar and optical measurements spanning 51 years, reveals a 20-minute interval in March 2880 when there could be a nonnegligible probability of the 1-kilometer object colliding with Earth. Trajectory knowledge remains accurate until then because of extensive astrometric data, an inclined orbit geometry that reduces in-plane perturbations, and an orbit uncertainty space modulated by gravitational resonance. The approach distance uncertainty in 2880 is determined primarily by uncertainty in the accelerations arising from thermal re-radiation of solar energy absorbed by the asteroid. Those accelerations depend on the spin axis, composition, and surface properties of the asteroid, so that refining the collision probability may require direct inspection by a spacecraft. PMID:11935024

Giorgini, J D; Ostro, S J; Benner, L A M; Chodas, P W; Chesley, S R; Hudson, R S; Nolan, M C; Klemola, A R; Standish, E M; Jurgens, R F; Rose, R; Chamberlin, A B; Yeomans, D K; Margot, J L



Studies of the {boldsymbol{bar{K}}}boldsymbol{N} interaction at DA?NE  

NASA Astrophysics Data System (ADS)

AMADEUS is an experiment planned to be performed at the DA?NE e+e- collider of the Frascati National Laboratories (Italy) of INFN, to investigate the antikaon-nuclei interaction at low energies. AMADEUS will perform, for the first time, full-acceptance studies of antikaon interaction in light nuclei, including a complete experimental program for the case of the kaonic nuclear clusters. The study of the absorption of antikaon by the nucleus will provide information concerning the bar{K}N interaction and the modification of the kaon mass in the nuclear medium. The experiment is being preceded by the study of the hadronic interactions of K- in the 4He of the drift chamber from the KLOE experiment data.

Vázquez Doce, O.; Bazzi, M.; Berucci, C.; Bombelli, L.; Bragadireanu, A. M.; Cargnelli, M.; Clozza, A.; Curceanu (Petrascu), C.; d'Uffizi, A.; Fiorini, C.; Ghio, F.; Guaraldo, C.; Iliescu, M.; Ishiwatari, T.; Sandri, P. Levi; Longoni, A.; Marton, J.; Okada, S.; Pietreanu, D.; Piscicchia, K.; Poli Lener, M.; Ponta, T.; Rizzo, A.; Romero Vidal, A.; Sbardella, E.; Scordo, A.; Shi, H.; Sirghi, D. L.; Sirghi, F.; Tatsuno, H.; Widmann, E.; Zmeskal, J.


Studies of the {boldsymbol{bar{K}}}boldsymbol{N} interaction at DA?NE  

NASA Astrophysics Data System (ADS)

AMADEUS is an experiment planned to be performed at the DA?NE e+e- collider of the Frascati National Laboratories (Italy) of INFN, to investigate the antikaon-nuclei interaction at low energies. AMADEUS will perform, for the first time, full-acceptance studies of antikaon interaction in light nuclei, including a complete experimental program for the case of the kaonic nuclear clusters. The study of the absorption of antikaon by the nucleus will provide information concerning the bar{K}N interaction and the modification of the kaon mass in the nuclear medium. The experiment is being preceded by the study of the hadronic interactions of K- in the 4He of the drift chamber from the KLOE experiment data.

Vázquez Doce, O.; Bazzi, M.; Berucci, C.; Bombelli, L.; Bragadireanu, A. M.; Cargnelli, M.; Clozza, A.; Curceanu (Petrascu), C.; d'Uffizi, A.; Fiorini, C.; Ghio, F.; Guaraldo, C.; Iliescu, M.; Ishiwatari, T.; Sandri, P. Levi; Longoni, A.; Marton, J.; Okada, S.; Pietreanu, D.; Piscicchia, K.; Poli Lener, M.; Ponta, T.; Rizzo, A.; Romero Vidal, A.; Sbardella, E.; Scordo, A.; Shi, H.; Sirghi, D. L.; Sirghi, F.; Tatsuno, H.; Widmann, E.; Zmeskal, J.



Search for Kaonic Nuclei at DA?NE2:. the Amadeus Project  

NASA Astrophysics Data System (ADS)

The planned upgrade of DA?NE at LNF with a peak luminosity in the order of L = 1033cm-2s-1 will be the ideal machine to search for and prove the existence of antikaon-mediated deeply bound nuclear cluster. Antikaon-mediated deeply bound nuclear clusters will allow the study of most important problems in hadron physics today, namely, how the hadron masses and hadron interactions change in the nuclear medium and what is the structure of cold dense hadronic matter. AMADEUS will use the KLOE detector with an additional dedicated target and detector system around the interaction zone to perform exclusive measurements - all particles in the formation and decay processes of deeply bound nuclear clusters will be detected. These measurements will prove beyond doubt the anticipated formation production of antikaon-mediated deeply bound nuclear clusters.

Zmeskal, Johann


TSPO 18 kDa (PBR) Targeted Photosensitizers for Cancer Imaging (PET) and PDT  

PubMed Central

Translocator protein (TSPO) 18 kDa overexpression has been observed in a large variety of human cancers, especially breast cancers. PK 11195, an isoquinoline analogue, is one of the ligands of highest TSPO binding affinity. Due to the long biological half life of our photosensitizers, there is a need to label them with a long lived radioisotope, for example I-124. Our objectives are to find translocator protein targeted photosensitizers for both tumor imaging (PET) and photodynamic therapy (PDT). I-PK 11195 is conjugated with the tumor avid photosensitizer HPPH. We find that those two tumor avid components complement each other and make the conjugate molecule even more tumor avid; compared to the photosensitizer itself, the conjugate is found to show improved PDT efficacy. It is concluded that I-PK 11195 can be a good vehicle to deliver radionuclide and photosensitizer to TSPO overexpressed tumor regions. Such conjugates could be useful for both tumor imaging (PET) and PDT.



Parameter-free stark broadening of hydrogen lines in DA white dwarfs  

NASA Astrophysics Data System (ADS)

We present new calculations for the Stark broadening of the hydrogen line profiles in the dense atmospheres of white dwarf stars. Our improved model is based on the unified theory of Stark broadening from Vidal, Cooper & Smith (1970), but it also includes non-ideal gas effects from the Hummer & Mihalas (1988) occupation probability formalism directly inside the line profile calculations. This approach improves upon previous calculations that relied on the use of an ad-hoc free parameter to describe the dissolution of the line wing opacity in the presence of high electric microfields in the plasma. We present here the first grid of model spectra for hot (Teff >= 12, 000 K) DA white dwarfs that has no free parameters. The atmospheric parameters obtained from optical and UV spectroscopic observations using these improved models are shown to differ substantially from those published in previous studies.

Tremblay, P.-E.; Bergeron, P.; Dupuis, J.



Prediction of clinical outcome with microarray data: a partial least squares discriminant analysis (PLS-DA) approach  

Microsoft Academic Search

Partial least squares discriminant analysis (PLS-DA) is a partial least squares regression of a set Y of binary variables describing the categories of a categorical variable on a set X of predictor variables. It is a compromise between the usual discriminant analysis and a discriminant analysis on the significant principal components of the predictor variables. This technique is specially suited

Miguel Pérez-Enciso; Michel Tenenhaus



Phosphorus in agroforestry systems : a contribution to sustainable agriculture in the Zona da Mata of Minas Gerais, Brazil  

Microsoft Academic Search

The Zona da Mata is a region situated in the domain of the Atlantic Coastal Rainforest in the southeast of the state of Minas Gerais, Brazil. This domain stretches along the Brazilian coast from north to south and ranks among the top five of the 25 biodiversity hotspots, the richest and the most threatened reservoirs of plant and animal life

I. M. Cardoso



32 CFR Appendix D to Part 623 - Certificate for Signature by an Alternate (DA Form 4881-1-R)  

Code of Federal Regulations, 2013 CFR

...Certificate for Signature by an Alternate (DA Form 4881-1-R) D Appendix D to Part 623 National Defense Department of Defense...AND EQUIPMENT LOAN OF ARMY MATERIEL Pt. 623, App. D Appendix D to Part 623âCertificate for Signature...



Crystal structure of a 30 kDa C-terminal fragment from the ? chain of human fibrinogen  

Microsoft Academic Search

Background: Blood coagulation occurs by a cascade of zymogen activation resulting from minor proteolysis. The final stage of coagulation involves thrombin generation and limited proteolysis of fibrinogen to give spontaneously polymerizing fibrin. The resulting fibrin network is covalently crosslinked by factor XIIIa to yield a stable blood clot. Fibrinogen is a 340 kDa glycoprotein composed of six polypeptide chains, (???)2,

Vivien C Yee; Kathleen P Pratt; Hélène CF Côté; Isolde Le Trong; Dominic W Chung; Earl W Davie; Ronald E Stenkamp; David C Teller



Carbon-14 Dating of a Mummy from 'Caverna DA Babilonia', Rio Novo Country, South of Minas Gerais (MG, Brazil).  

National Technical Information Service (NTIS)

The vegetable fibers of a cloth wrapping a mummy of a woman, found in 'Caverna da Babilonia' (MG, Brazil), were dated with carbon-14. There is strong evidence that it is a pre-colombian mummy since the age of the sample is 600 + - 80 years (1sigma). (Atom...

M. C. M. C. Beltrao J. Danon G. Poupeau



Topology and dynamics of the 10 kDa C-terminal domain of DnaK in solution.  

PubMed Central

Hsp70 molecular chaperones contain three distinct structural domains, a 44 kDa N-terminal ATPase domain, a 17 kDa peptide-binding domain, and a 10 kDa C-terminal domain. The ATPase and peptide binding domains are conserved in sequence and are functionally well characterized. The function of the 10 kDa variable C-terminal domain is less well understood. We have characterized the secondary structure and dynamics of the C-terminal domain from the Escherichia coli Hsp70, DnaK, in solution by high-resolution NMR. The domain was shown to be comprised of a rigid structure consisting of four helices and a flexible C-terminal subdomain of approximately 33 amino acids. The mobility of the flexible region is maintained in the context of the full-length protein and does not appear to be modulated by the nucleotide state. The flexibility of this region appears to be a conserved feature of Hsp70 architecture and may have important functional implications. We also developed a method to analyze 15N nuclear spin relaxation data, which allows us to extract amide bond vector directions relative to a unique diffusion axis. The extracted angles and rotational correlation times indicate that the helices form an elongated, bundle-like structure in solution.

Bertelsen, E. B.; Zhou, H.; Lowry, D. F.; Flynn, G. C.; Dahlquist, F. W.



Effects of Monoclonal Antibody Against Porcine 40-kDa Adipocyte-Specific Membrane Protein on Endocrine Secretion in Pigs  

Microsoft Academic Search

The present study was to investigate the effect of monoclonal antibody against porcine 40-kDa adipocyte-specific membrane protein on endocrine secretion in pigs, in order to provide the evidence for application of this antibody to reduce excessive fat deposition in pig production. 40 Landrace × Saba pigs were randomly divided into 8 groups: 2 control groups were given saline with 10

Ling-yun LIU; Hong-mei HU; Su-mei ZHAO; Xi ZHANG; Gang DUAN; Shi-zheng GAO



Uma Análise Tipológica da Dinâmica dos Arranjos e Sistemas Produtivos e Inovativos Locais (Aspil?S) Do Nordeste do Brasil  

Microsoft Academic Search

O presente trabalho tem como objetivo apresentar a dinâmica produtiva e inovativa dos Arranjos e Sistemas Produtivos e Inovativos Locais (ASPILs), tendo como foco os ASPILs nordestinos. Para tanto, foi utilizado o arcabouço da teoria evolucionária tendo por base o conceito de Sistema Nacional de Inovação do qual gerou a conceituação de ASPIL. Assim, buscou-se apresentar a dinâmica de arranjos

Diogo Araújo Sampaio


Seasonal variations of 226Ra and 222Rn in mineral spring waters of Aguas da Prata, Brazil  

Microsoft Academic Search

In this paper the activity concentrations of 226Ra and 222Rn were assayed in the mineral spring waters of Aguas da Prata in order to evaluate the seasonal variations of such radionuclides. The results obtained were related to the chemical composition of the water as well as to the lithology of the aquifer and temperature. Higher activity concentrations up to 1.8

Joselene De Oliveira; Barbara Mazzilli; Marian Helena De Oliveira Sampa; Bernadete Silva



Temperatures, gravities, and masses for a sample of bright DA white dwarfs and the initial-to-final mass relation  

Microsoft Academic Search

The atmospheric parameters, Teff and log g, have been determined for a sample of 52 hydrogen-line (DA) white dwarfs by fitting optical spectra to theoretical model atmospheres. Multiple observations of each star allow an estimation of the accuracy of the method: the average external errors are 490 K in Teff and 0.06 in log g. Masses have been derived using

Angela Bragaglia; Alvio Renzini; P. Bergeron



Rapid communication Variable 10Be fluxes in lacustrine sediments from Tristan da Cunha, South Atlantic: a solar record?  

Microsoft Academic Search

A 650-yr-long sediment sequence from a crater lake on Tristan da Cunha, South Atlantic, was analysed for its 10Be content. Based on 14 C dating, and sedimentary, geochemical, magnetic and palynological records, the period between 900 and 1450 AD appears to have been unusually stable in terms of sedimentation and vegetation and therefore this period was chosen for analysis of

Karl Ljunga; Peter W. Kubikd



Microsoft Academic Search

Resumo As patologias apresentadas por algumas estruturas e a necessidade de aumento da capacidade de carga de outras fizeram com que diversas técnicas fossem desenvolvidas para o reforço de vigas de concreto armado. Uma delas é a incorporação e protensão de cabos externos. O grande diferencial desta técnica quando comparada às demais é o seu caráter ativo, ou seja, por

Tatiana Gesteira; Martins de Almeida; João Bento de Hanai


Metáforas de Lula e Alckmin nos debates de 2006 em uma perspectiva da Lingüística de Corpus1  

Microsoft Academic Search

RESUMO: O objetivo deste trabalho é contrastar o uso de metáforas pelos dois candidatos no segundo turno das eleições presidenciais de 2006, Luís Inácio Lula da Silva e Geraldo Alckmin, para saber até que ponto Lula, que foi vitorioso nas urnas, usou metáforas de modo mais eficaz do que o outro candidato durante os debates televisados. Foi colhido um corpus

Tony Berber Sardinha



Microsoft Academic Search

RESUMO: A suíte Serrinha localiza-se na porção meridional do Cráton São Francisco e é representada pelo quartzo diorito do Brito, granodiorito Brumado de Cima, granodiorito Brumado de Baixo, dois corpos granofíricos e dois corpos subvulcânicos - vulcânicos félsicos. As relações de campo apontam que os corpos da suíte Serrinha são intrusivos em rochas metamáficas, metaultramáficas e metassedimentares do greenstone belt



Delimitação do topo de aqüífero na região de Porto Seguro - Bahia através da inversão de sondagens elétricas verticais  

Microsoft Academic Search

O presente trabalho compreendeu estudos geofísicos conduzidos com o método da eletrorresistividade na região de Porto Seguro (BA), que tiveram o objetivo de localizar o aqüífero mais promissor para a exploração de água subterrânea naquela região. Os trabalhos recobriram uma área de aproximadamente 60km2 situada imediatamente ao norte de Porto Seguro. A área pesquisada insere-se num contexto geológico de ambiente

Alexandre C. Monteiro; Milton J. Porsani



The Late Holocene upper montane cloud forest and high altitude grassland mosaic in the Serra da Igreja, Southern Brazil.  


Many soils of the highlands of Serra do Mar, as in other mountain ranges, have thick histic horizons that preserve high amounts of carbon. However, the age and constitution of the organic matter of these soils remain doubtful, with possible late Pleistocene or Holocene ages. This study was conducted in three profiles (two in grassland and one in forest) in Serra da Igreja highlands in the state of Paraná. We performed ?13C isotope analysis of organic matter in soil horizons to detect whether C3 or C4 plants dominated the past communities and 14C dating of the humin fraction to obtain the age of the studied horizons. C3 plants seem to have dominated the mountain ridges of Serra da Igreja since at least 3,000 years BP. Even though the Serra da Igreja may represents a landscape of high altitude grasslands in soils containing organic matter from the late Pleistocene, as reported elsewhere in Southern and Southeastern Brazil, our results indicate that the sites studied are at least from the beginning of the Late Holocene, when conditions of high moisture enabled the colonization/recolonization of the Serra da Igreja ridges by C3 plants. This is the period, often reported in the literature, when forests advanced onto grasslands and savannas. PMID:23828336

Scheer, Maurício B; Curcio, Gustavo R; Roderjan, Carlos V



Impact of Hemp Production on the Development of the Municipalities of Araras, Leme, Santa Cruz DA Conceicao and Pirassununga.  

National Technical Information Service (NTIS)

Data from LANDSAT-5 and SPOT-1 satellites were utilized to update soil use in 4 municipalities of Sao Paulo (Araras, Leme, Santa Cruz da Conceicao, and Pirassununga) which were posteriorly compared, the mapping performed in 1978 was studied for the dynami...

M. Covre C. B. S. Veiga M. Morgado R. Dossantos R. L. V. Rodrigues



A prothrombin activator from Bothrops erythromelas (jararaca-da-seca) snake venom: characterization and molecular cloning.  

PubMed Central

A novel prothrombin activator enzyme, which we have named 'berythractivase', was isolated from Bothrops erythromelas (jararaca-da-seca) snake venom. Berythractivase was purified by a single cation-exchange-chromatography step on a Resource S (Amersham Biosciences) column. The overall purification (31-fold) indicates that berythractivase comprises about 5% of the crude venom. It is a single-chain protein with a molecular mass of 78 kDa. SDS/PAGE of prothrombin after activation by berythractivase showed fragment patterns similar to those generated by group A prothrombin activators, which convert prothrombin into meizothrombin, independent of the prothrombinase complex. Chelating agents, such as EDTA and o -phenanthroline, rapidly inhibited the enzymic activity of berythractivase, like a typical metalloproteinase. Human fibrinogen A alpha-chain was slowly digested only after longer incubation with berythractivase, and no effect on the beta- or gamma-chains was observed. Berythractivase was also capable of triggering endothelial proinflammatory and procoagulant cell responses. von Willebrand factor was released, and the surface expression of both intracellular adhesion molecule-1 and E-selectin was up-regulated by berythractivase in cultured human umbilical-vein endothelial cells. The complete berythractivase cDNA was cloned from a B. erythromelas venom-gland cDNA library. The cDNA sequence possesses 2330 bp and encodes a preproprotein with significant sequence similarity to many other mature metalloproteinases reported from snake venoms. Berythractivase contains metalloproteinase, desintegrin-like and cysteine-rich domains. However, berythractivase did not elicit any haemorrhagic response. These results show that, although the primary structure of berythractivase is related to that of snake-venom haemorrhagic metalloproteinases and functionally similar to group A prothrombin activators, it is a prothrombin activator devoid of haemorrhagic activity. This is a feature not observed for most of the snake venom metalloproteinases, including the group A prothrombin activators.

Silva, Marcia B; Schattner, Mirta; Ramos, Celso R R; Junqueira-de-Azevedo, Inacio L M; Guarnieri, Miriam C; Lazzari, Maria A; Sampaio, Claudio A M; Pozner, Roberto G; Ventura, Janaina S; Ho, Paulo L; Chudzinski-Tavassi, Ana M



Diffusion into human islets is limited to molecules below 10 kDa.  


Isolated islets are important tools in diabetes research and are used for islet transplantation as a treatment for type 1 diabetes. Yet these cell clusters have a dramatic diffusion barrier that leads to core cell death. Computer modeling has provided theoretical size limitations, but little has been done to measure the actual rate of diffusion in islets. The purpose of this study was to directly measure the diffusion barrier in intact human islets and determine its role in restricting insulin secretion. Impeded diffusion into islets was monitored with fluorescent dextran beads. Dextran beads of 10-70 kDa failed to diffuse into the core of the intact islets, while 0.9 kDa probe was observed within the core of smaller islets. Diffusion of the fluorescent form of glucose, 2-NBDG, had similar diffusion limitations as the beads, with an average intra-islet diffusion rate of 1.5 ± 0.2 ?m/min. The poor diffusion properties were associated with core cell death from necrosis, not apoptosis. Short-term exposure to a mild papain/0 Ca(2+) cocktail, dramatically reduced the diffusion barrier so that all cells within islets were exposed to media components. Lowering the diffusion barrier increased the immediate and long-term viability of islet cells, and tended to increase the amount of insulin released, especially in low glucose conditions. However, it failed to improve the large islet's glucose-stimulated insulin secretion. Thus, the islet diffusion barrier leads to low viability and poor survival of large islets, but is not solely responsible for the reduced insulin secretion of large isolated islets. PMID:22717091

Williams, S J; Schwasinger-Schmidt, T; Zamierowski, D; Stehno-Bittel, L



Human monoclonal islet specific autoantibodies share features of islet cell and 64 kDa antibodies.  


The first human monoclonal islet cell antibodies of the IgG class (MICA 1-6) obtained from an individual with Type 1 (insulin-dependent) diabetes mellitus were cytoplasmic islet cell antibodies selected by the indirect immunofluorescence test on pancreas sections. Surprisingly, they all recognized the 64 kDa autoantigen glutamate decarboxylase. In this study we investigated which typical features of cytoplasmic islet cell antibodies are represented by these monoclonals. We show by double immunofluorescence testing that MICA 1-6 stain pancreatic beta cells which is in agreement with the beta-cell specific expression of glutamate decarboxylase. In contrast an islet-reactive IgM monoclonal antibody obtained from a pre-diabetic individual stained all islet cells but lacked the tissue specificity of MICA 1-6 and must therefore be considered as a polyreactive IgM-antibody. We further demonstrate that MICA 1-6 revealed typical features of epitope sensitivity to biochemical treatment of the target tissue which has been demonstrated for islet cell antibodies, and which has been used to argue for a lipid rather than a protein nature of target antigens. Our results provide direct evidence that the epitopes recognized by the MICA are destroyed by methanol/chloroform treatment but reveal a high stability to Pronase digestion compared to proinsulin epitopes. Conformational protein epitopes in glutamate decarboxylase therefore show a sensitivity to biochemical treatment of sections such as ganglioside epitopes. MICA 1-6 share typical features of islet cell and 64 kDa antibodies and reveal that glutamate decarboxylase-reactive islet cell antibodies represent a subgroup of islet cell antibodies present in islet cell antibody-positive sera. PMID:7691667

Richter, W; Eiermann, T H; Endl, J; Seissler, J; Wolfahrt, S; Brandt, M; Jungfer, H; Scherbaum, W A



Extraction of Features from High-resolution 3D LiDaR Point-cloud Data  

NASA Astrophysics Data System (ADS)

Airborne and tripod-based LiDaR scans are capable of producing new insight into geologic features by providing high-quality 3D measurements of the landscape. High-resolution LiDaR is a promising method for studying slip on faults, erosion, and other landscape-altering processes. LiDaR scans can produce up to several billion individual point returns associated with the reflection of a laser from natural and engineered surfaces; these point clouds are typically used to derive a high-resolution digital elevation model (DEM). Currently, there exist only few methods that can support the analysis of the data at full resolution and in the natural 3D perspective in which it was collected by working directly with the points. We are developing new algorithms for extracting features from LiDaR scans, and present method for determining the local curvature of a LiDaR data set, working directly with the individual point returns of a scan. Computing the curvature enables us to rapidly and automatically identify key features such as ridge-lines, stream beds, and edges of terraces. We fit polynomial surface patches via a moving least squares (MLS) approach to local point neighborhoods, determining curvature values for each point. The size of the local point neighborhood is defined by a user. Since both terrestrial and airborne LiDaR scans suffer from high noise, we apply additional pre- and post-processing smoothing steps to eliminate unwanted features. LiDaR data also captures objects like buildings and trees complicating greatly the task of extracting reliable curvature values. Hence, we use a stochastic approach to determine whether a point can be reliably used to estimate curvature or not. Additionally, we have developed a graph-based approach to establish connectivities among points that correspond to regions of high curvature. The result is an explicit description of ridge-lines, for example. We have applied our method to the raw point cloud data collected as part of the GeoEarthScope B-4 project on a section of the San Andreas Fault (Segment SA09). This section provides an excellent test site for our method as it exposes the fault clearly, contains few extraneous structures, and exhibits multiple dry stream-beds that have been off-set by motion on the fault.

Keller, P.; Kreylos, O.; Hamann, B.; Kellogg, L. H.; Cowgill, E. S.; Yikilmaz, M. B.; Hering-Bertram, M.; Hagen, H.



Spontaneously hypertensive rats and platelet Ca(2+)-ATPases: specific up-regulation of the 97 kDa isoform.  

PubMed Central

The use of platelets instead of smooth muscle cells (SMC) to study the abnormal Ca2+ handling found in hypertension was investigated using spontaneously hypertensive rats (SHR). We studied the regulation of platelet Ca(2+)-ATPases, as we have recently demonstrated that human platelets, like SMC, contain the Ca(2+)-ATPase isoform termed SERCA2-b (sarco-endoplasmic reticulum Ca(2+)-ATPase). In mixed membranes isolated from platelets of normotensive Wistar-Kyoto (WKY) rats and SHR, total Ca(2+)-ATPase activity was found to be 43% higher in SHR than in WKY rats. By the use of autophosphorylation of rat platelet Ca(2+)-ATPases with [gamma-32P]ATP, followed by SDS/PAGE and Western blotting, we found that rat platelets express two distinct Ca(2+)-ATPases: a 100 kDa isoform, recognized by a SERCA2-b-specific anti-peptide antibody, and a 97 kDa isoform, specifically recognized by a polyclonal anti-SERCA antibody. Comparative analysis of platelet membrane Ca(2+)-ATPases from WKY rats and SHR demonstrated that the expression of the SERCA2-b isoform did not change significantly (128 +/- 22%), whereas that of the 97 kDa isoform reached 300 +/- 35% in SHR when compared with WKY rats. We concluded that the upregulation of total platelet Ca(2+)-ATPases in SHR is not due to the 100 kDa SERCA2-b isoform found in SMC, but is specific to the 97 kDa Ca(2+)-ATPase isoform which is not present in SMC. Therefore platelets should be used with extreme caution as a surrogate model of vascular smooth muscle Ca2+ homeostasis. Images Figure 2 Figure 3

Papp, B; Corvazier, E; Magnier, C; Kovacs, T; Bourdeau, N; Levy-Toledano, S; Bredoux, R; Levy, B; Poitevin, P; Lompre, A M



A 60 kDa polypeptide of skeletal-muscle sarcoplasmic reticulum is a calmodulin-dependent protein kinase that associates with and phosphorylates several membrane proteins.  

PubMed Central

Activation of a calmodulin (CaM)-dependent protein kinase associated with rabbit skeletal-muscle sarcoplasmic reticulum (SR) results in the phosphorylation of polypeptides of 450, 360, 165, 105, 89, 60, 34 and 20 kDa. Radioligand-binding studies indicated that a membrane-bound 60 kDa polypeptide contained both CaM- and ATP-binding domains. Under renaturing conditions on nitrocellulose blots, the 60 kDa polypeptide of the membrane exhibited CaM-dependent autophosphorylation activity, suggesting that it was the CaM-dependent protein kinase of SR. Ca2+/CaM-independent autophosphorylation of polypeptides of 62 and 45 kDa was found to occur in the light SR, whereas the Ca2+/CaM-dependent autophosphorylation activity was enriched in the heavy SR. Both these kinase activities were absent from transverse tubules, although these membranes were enriched in CaM-binding polypeptides of 160, 100 and 80 kDa. In the absence of Ca2+, CaM bound to a 33 kDa polypeptide of the membrane. The purified ryanodine receptor was not phosphorylated by the purified CaM kinase, although it was a substrate for protein kinase C. Affinity-purified antibodies to brain CaM kinase II cross-reacted with the 60 kDa polypeptide in Western blots and immunoprecipitated the 60 kDa polypeptide, along with the 360, 105, 89, 34 and 20 kDa phosphoproteins, from Nonidet-P-40-solubilized SR membranes. Antibodies raised against the 60 kDa kinase polypeptide did not cross-react with the other phosphoproteins, suggesting that these polypeptides were distinct and unrelated. Subcellular distribution of the 60 kDa kinase indicated the specific association of the polypeptide with the junctional-face membrane of SR. The CaM-dependent incorporation of 32P into various membrane proteins was inhibited by the CaM kinase II fragment (290-309), with an IC50 value of 2 nM for the inhibition of incorporation into the 60 kDa kinase polypeptide. Recent studies [Wang and Best (1992) Nature (London) 359, 739-741] have shown that a CaM kinase activity intrinsic to the membrane can inactivate the Ca(2+)-release channel of skeletal muscle SR. Since our results demonstrate that the 60 kDa polypeptide of SR is a CaM-dependent protein kinase, we suggest that this kinase, through its associations, may be responsible for gating the Ca(2+)-release channel. Images Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8

Leddy, J J; Murphy, B J; Qu-Yi; Doucet, J P; Pratt, C; Tuana, B S



Prevention of NSAID-Associated Gastroduodenal Injury in Healthy Volunteers-A Randomized, Double-Blind, Multicenter Study Comparing DA-9601 with Misoprostol  

PubMed Central

In addition to inhibiting cyclooxygenase and prostaglandin, nonsteroidal anti-inflammatory drugs (NSAIDs) may cause gastroduodenal injuries due to reactive oxygen species produced by recruited inflammatory cells. DA-9601 is a novel antioxidant with anti-inflammatory and cyto-protective effects. This study was conducted to compare the efficacy and safety of DA-9601 with misoprostol for preventing NSAID-associated gastroduodenal injury. In this randomized, double-blind, multicenter, noninferiority trial we compared the extents of protection of gastric and duodenal mucosae by endoscopy after 4 weeks of treatment with DA-9601 60 mg or misoprostol 200 µg three times daily, in subjects with normal baseline endoscopic findings who received an NSAID twice daily for 4 weeks. A total of 266 subjects were randomized to treatment. At week 4, the gastric protection rates with DA-9601 and misoprostol were 85.1% and 95.2%, respectively; the difference between the groups was -10.1% (var = 0.001), which was shown to indicate noninferiority of DA-9601 compared to misoprostol. Adverse events were lower in the DA-9601 group, 56.4% (95% CI, 48.0%-64.8%) than in the misoprostol group, 69.2% (95% CI, 61.3%-77.0%) (P = 0.031). DA-9601 is not inferior to misoprostol for preventing NSAID-associated gastroduodenal injury, and superior to it with respect to treatment-related side effects.

Lee, Kang Nyeong; Lee, Oh Young; Choi, Myung-Gyu; Choi, Seok Reyol; Lee, Dong Ho; Lee, Yong Chan; Kim, Tae Nyeun; Choi, Suck Chei; Rew, Jong Sun



A 130-kDa protein 4.1B regulates cell adhesion, spreading, and migration of mouse embryo fibroblasts by influencing actin cytoskeleton organization.  


Protein 4.1B is a member of protein 4.1 family, adaptor proteins at the interface of membranes and the cytoskeleton. It is expressed in most mammalian tissues and is known to be required in formation of nervous and cardiac systems; it is also a tumor suppressor with a role in metastasis. Here, we explore functions of 4.1B using primary mouse embryonic fibroblasts (MEF) derived from wild type and 4.1B knock-out mice. MEF cells express two 4.1B isoforms: 130 and 60-kDa. 130-kDa 4.1B was absent from 4.1B knock-out MEF cells, but 60-kDa 4.1B remained, suggesting incomplete knock-out. Although the 130-kDa isoform was predominantly located at the plasma membrane, the 60-kDa isoform was enriched in nuclei. 130-kDa-deficient 4.1B MEF cells exhibited impaired cell adhesion, spreading, and migration; they also failed to form actin stress fibers. Impaired cell spreading and stress fiber formation were rescued by re-expression of the 130-kDa 4.1B but not the 60-kDa 4.1B. Our findings document novel, isoform-selective roles for 130-kDa 4.1B in adhesion, spreading, and migration of MEF cells by affecting actin organization, giving new insight into 4.1B functions in normal tissues as well as its role in cancer. PMID:24381168

Wang, Jie; Song, Jinlei; An, Chao; Dong, Wenji; Zhang, Jingxin; Yin, Changcheng; Hale, John; Baines, Anthony J; Mohandas, Narla; An, Xiuli



Pharmacokinetic interaction between DA-8159, a new erectogenic, and metformin in rats: competitive inhibition of metabolism via hepatic CYP3A1/2  

PubMed Central

Background and purpose: Erectile dysfunction is highly prevalent in diabetic patients and PDE V inhibitors are effective and safe for the treatment of erectile dysfunction in men with diabetes. Therefore, in this study we investigated whether a pharmacokinetic interaction occurs between DA-8159 and metformin, as both drugs are metabolized via hepatic CYP3A1/2 in rats. Experimental approach: DA-8159 (30?mg?kg–1) and metformin (100?mg?kg–1), both separately and together, were administered to rats either intravenously or orally. The V max, K m, CLint, apparent inhibition constants (K i), [I]/K i and concentrations of each drug in the liver and intestine were then measured. Key results: After i.v. administration of both drugs simultaneously, the AUC of DA-8159 and metformin was significantly greater (21.2 and 33.9% increase for DA-8159 and metformin, respectively) than that of each drug alone. After p.o. administration of the drugs, the AUC of metformin was also significantly greater (20.7% increase) in the presence of DA-8159 than in its absence. However, the AUC of DA-8159 was similar in the absence and presence of metformin. Conclusions and implications: The significantly greater AUC of metformin and DA-8159 after i.v. administration of both drugs and of metformin after p.o. administration of both drugs is probably due to competitive inhibition for the metabolism of these drugs via hepatic CYP3A1/2. However, the similar AUCs of DA-8159 in the absence and presence of metformin, after p.o. administration, indicates that the dose of metformin used was insufficient to inhibit the hepatic and intestinal metabolism of DA-8159.

Choi, Y H; Chung, S J; Lee, M G



Percepção astronómica de um grupo de alunos do ensino médio de uma escola da rede estadual de São Paulo  

NASA Astrophysics Data System (ADS)

Sendo a Astronomia uma das cièncias mais antigas da humanidade, e considerando sua importáncia histórica e cultural, é de extrema releváncia que tópicos relacionados a ela sejam tratados nas escolas. Embora os Parámetros Curriculares Nacionais do Ensino Médio (PCN-EM) e as Orientaçiacute;es Complementares aos Parámetros (PCN+) apontem a importáncia de uma abordagem significativa de conceitos relacionados à Astronomia nas aulas de Física, muitos estudantes terminam o Ensino Médio (EM) sem compreender a razão de certos acontecimentos de origem celeste, ainda que estes façam parte de seu cotidiano e sejam alvos da curiosidade natural dos jovens. Da observação dessa curiosidade em alunos de uma escola pública paulista, na cidade de Suzano, surgiu o interesse em investigar os conhecimentos básicos em Astronomia dos alunos do Ensino Médio desta escola, constituindo-se este como principal objetivo desta pesquisa. Para tanto foi elaborado um questionário de múltipla escolha aplicado inicialmente a 34 alunos do primeiro ano e, posteriormente, a mais 310, distribuídos entre as très séries do Ensino Médio dos períodos matutino e noturno. Dessa forma, observou-se que 73,9% dos estudantes identificaram o Sol como sendo uma estrela, 67,1% mostraram compreender a sucessão entre dia e noite e 52,3% relacionaram o Big Bang à origem do Universo. Em contrapartida, apenas 34,5% relacionaram as estaçíes do ano à inclinação do eixo de rotação da Terra, 21,3% indicaram a influència gravitacional simultánea da Lua e do Sol como responsável pelo fenòmeno das marés, 24,5% indicaram corretamente quais são os objetos celestes mais próximos da Terra, 36,1% identificaram ano-luz como uma medida de distáncia e 34,2% reconheceram as estrelas cadentes como meteoros, evidenciando-se assim o pequeno discernimento dos estudantes quanto aos fenòmenos e termos astronòmicos do cotidiano. Além disso, foram comparadas as respostas de alun! os de diferentes séries e períodos, observando-se, dentre ou tras coi sas, que os estudantes do terceiro ano apresentam um percentual de acertos semelhante ao dos alunos do primeiro, caracterizando que a abordagem de tópicos relacionados à astronomia no Ensino Médio não tem contribuído de forma significativa para uma maior compreensão dos fenòmenos e conceitos.

de Oliveria, E. F.; Voelzke, M. R.



Cloning and characterization of a 77-kDa oestrogen receptor isolated from a human breast cancer cell line.  

PubMed Central

We have cloned and characterized a 77-kDa oestrogen receptor (ER) from an oestrogen-independent subclone of the MCF-7 human breast cancer cell line. This receptor contains an in-frame, tandem duplication of exons 6 and 7, located in the steroid-binding domain of the ER. This mutation has abrogated ligand binding, but not DNA binding, in this mutant ER. We previously described the partial structure of a unique oestrogen receptor (ER) that is expressed in an oestrogen-independent MCF-7:2A subclone of the breast cancer cell line MCF-7 (Pink JJ, Wu SQ, Wolf DM, Bilimoria MM, Jordan VC 1996a, Nucleic Acids Res 24 962-969). Sequence analyses determined the molecular weight of this 80-kDa ER to be 77 kDa, and hereafter this protein will be designated as ER77. Examination of the entire coding sequence of the ER77 mRNA indicates that it contains a tandem duplication of exons 6 and 7. Using a coupled transcription/translation system, a 77-kDa ER, which corresponds to the protein observed in the MCF-7:2A cells, was expressed. The ER77 protein does not bind the ligands [3H] oestradiol or [3H]tamoxifen aziridine. In DNA binding gel shift assays, the in vitro synthesized ER77 binds to a consensus vitellogenin A2 oestrogen-response element. In transient transfection experiments, the mutant ER, alone or in combination with the wild-type ER, does not induce expression of an oestrogen-responsive luciferase reporter construct. In fact, expression of the ER77 in the ER-positive T47D:A18 cell line inhibits E2-induced luciferase expression. Overexpression of wild-type ER in T47D:A18 cells leads to elevated constitutive expression of the luciferase reporter, which was inhibited by co-transfection with ER77. These data suggest that the ER77 can interfere with normal ER activity and does not act as a constitutive activator of oestrogen-independent growth in MCF-7:2A cells. Consequently, the constitutive growth observed in MCF-7:2A cells is probably the result of other ER-mediated pathways. Images Figure 2 Figure 3 Figure 4 Figure 5

Pink, J. J.; Fritsch, M.; Bilimoria, M. M.; Assikis, V. J.; Jordan, V. C.



Matrix metalloproteinase degradation of elastin, type IV collagen and proteoglycan. A quantitative comparison of the activities of 95 kDa and 72 kDa gelatinases, stromelysins-1 and -2 and punctuated metalloproteinase (PUMP).  

PubMed Central

The abilities of the matrix metalloproteinases 95 kDa and 72 kDa gelatinases (type IV collagenases), stromelysins-1 and -2 and punctuated metalloproteinase (PUMP) to degrade insoluble elastin, type IV collagen films and proteoglycan have been compared. The gelatinases and PUMP were markedly more active in the degradation of elastin than were the stromelysins. PUMP and the stromelysins were more potent proteoglycan-degrading enzymes. All of the enzymes studied degraded soluble native type IV collagen, but the gelatinases were more effective at higher temperatures. These quantitative data allow an analysis of the potential relative roles of these metalloproteinases in the breakdown of the key components of connective tissue matrices. Images Fig. 1.

Murphy, G; Cockett, M I; Ward, R V; Docherty, A J



On Ensino da Astronomia no Ensino Médio sob Diferentes Abordagens Metodológicas  

NASA Astrophysics Data System (ADS)

O presente trabalho, sobre a intervenção de metodologias de ensino, foi desenvolvido na Escola Estadual Colônia dos Pescadores, na cidade de Caraguatatuba - SP, em três turmas do terceiro ano do Ensino Médio, perfazendo um total de 119 educandos, entre 16 e 19 anos. Antes de iniciar-se a intervenção, um questionário de vinte perguntas objetivas e dissertativas foi desenvolvido, aplicado pelo professor da classe, que ministrou as aulas correspondentes. Este questionário foi o mesmo em todas as três classes com o objetivo de diagnosticar o conhecimento prévio dos alunos sobre Astronomia. Começando a intervenção nas turmas, o professor envolvido usou três diferentes metodologias de ensino: (A) em forma de seminários, elaborados e apresentados pelos alunos, nos quais o professor fazia apenas as intervenções necessárias, (B) na forma tradicional, com a ajuda de multimídia para o desenvolvimento das aulas e a terceira (C) a tradicional, fazendo uso exclusivo de lousa e giz. No final do trabalho os alunos responderam o mesmo questionário novamente, de modo que os três métodos utilizados puderam ser comparados. Os resultados apresentados após a intervenção foram melhores que os resultados iniciais indicando a ocorrência de uma aprendizagem significativa. Quando os estudantes foram inicialmente questionados sobre quantos planetas existem no nosso sistema solar, a classe A obteve 39% de respostas certas, a classe B 48% e a classe C 46%, mas após o desenvolvimento das atividades, as classes obtiveram respectivamente 94%, 97 % e 90% de aproveitamento. No término do bimestre, foi sugerido aos educandos que elaborassem uma história em quadrinhos, a qual serviu para averiguar se os conceitos inicialmente observados foram alterados e se novos foram agregados. A análise das histórias foi dividida em três partes: Criatividade; Temas abordados; Emprego correto dos conceitos estudados. Ao final quatorze histórias foram confeccionadas. O aprendizado foi tão significativo que os alunos produzira m histórias em quadrinhos, com informações muito ricas. Outro dado muito importante é que, apesar da Astronomia ser um tema amplamente recomendado, não é ensinado nem em escolas de ensino fundamental nem no ensino médio.

Voelzke, Marcos Rincon; Albrecht, Evonir



Quartz grain surface analysis for paleoenvironmental reconstruction in Western Iberia mountain environments (Serra da Estrela, Portugal).  

NASA Astrophysics Data System (ADS)

This work deals with the genesis and paleoenvironmental significance of relict slope deposits in Serra da Estrela, Central Portugal and is part of a wider sedimentological and dating approach. The aim is to identify what microstructures indicate frost weathering, witch mechanisms are responsible for their development and the effectiveness of frost weathering across an altitudinal profile. Our goals are to contribute to the better understanding of the morphogenetical significance of the different types of slope deposits, clarifying the role of cold environment processes and develop a chronological framework for the Late Quaternary evolution of the slope environment of the mountains of Western Iberia. Quartz grains, 0.5 mm in diameter, were collected from samples from stratified, head and debris-flow deposits. Cailleux (1942) analysis, with modifications from Gozdzik (1980), was performed allowing assessing frosting and rounding, as well as the effects of later frost weathering in vertical profiles from slope deposits. Grains were also analysed using a scanning electron microscope (SEM) following Mahaney (2002). The effects of frost weathering comprise several microstructures such as breakage blocks, conchoidal fractures and fresh surfaces. Breakage blocks microstructures are the most common. Two types of frost weathering were distinguished: weathering of the P type is mainly initiated on convex fragments of grains as the result of ice segregation, and F type is linked to the volumetric expansion of ice and usually observed within concave linear microstructures. The frost action index (FAI) was estimated from the rate of microstructures from frost weathering. FAI varies between 0 and 3. The values of the sediments from the slope deposits vary between 1.2 and 2.05. This approach was for the first time applied on slope deposits from Serra da Estrela. There is a clear correlation between deposits in weathering type. Samples that display intensive physical weathering in the Cailleux analysis, are linked to weathering of the P type (effect of grains moving relative to each other) and also have the highest value of frost-weathering intensity. We interpret these samples taken from paleosols and openwork gravels layers to be related to transport by solifluction processes.

Nieuwendam, Alexandre; Woronko, Barbara; Schaefer, Carlos; Vieira, Gonçalo



Granzyme B\\/Perforin-Mediated Apoptosis of Jurkat Cells Results in Cleavage of Poly(ADP-ribose) Polymerase to the 89-kDa Apoptotic Fragment and Less Abundant 64-kDa Fragment  

Microsoft Academic Search

Cytotoxic lymphocytes utilize granule associated serine proteases (granzymes) and perforin to induce apoptosis. Although the importance of granzyme B has been established by gene ablation experiments, biochemical events initiated by the granzyme remain enigmatic. We show here that exposure of Jurkat cells to granzyme B and perforin results in cleavage of poly(ADP-ribose) polymerase to an apoptotic 89 kDa fragment and

Christopher J. Froelich; William L. Hanna; Guy G. Poirier; Patrick J. Duriez; Damien D'amours; Guy S. Salvesen; Emad S. Alnemri; William C. Earnshaw; Girish M. Shah



Heavy metals in bivalve mollusks collected from Da-Peng Bay Lagoon in south-southwestern Taiwan.  


In this study, concentrations of several heavy metals (Cu, Pb, Zn, Ni, Cr, and Cd) were measured in Katelysia hiantina, Anomalocardia squamosa, Perna viridis, Anadara antiquata, Paphia undulata, and Sanguinolaria diphos bivalve mollusks from Da-Peng Bay Lagoon near the south-southwestern coast of Taiwan. The metal pollution index (MPI) values were highest and lowest in winter and autumn, respectively. The MPI value in the viscera of P. viridis was higher than in muscles. In all four seasons, Zn concentrations in viscera and muscles of P. viridis were higher than for other metals. The capacities of A. squamosa to accumulate the concentrations of Cu, Ni, and Cr and of A. antiquata to accumulate concentrations of Pb, Zn, and Cd were significant. Analytical results suggested that A. squamosa and A. antiquata may be used as bioindicators for monitoring Cu, Ni, Cr, Pb, Zn, and Cd heavy-metal pollution in Da-Peng Bay Lagoon throughout the year. PMID:24555680

Shue, Meei-Fang; Chen, Wen-Der; Su, Chia-Chi; Lu, Ming-Chun



Differential expression of the 27 kDa cathepsin L-like cysteine protease in developmental stages of Spirometra erinacei  

PubMed Central

The 27 kDa cathepsin L-like cysteine protease of Spirometra erinacei plerocercoid is known to play an important function in tissue penetration, nutrient uptake and immune modulation in human sparganosis. In the present study, the expression of this enzyme was examined at different developmental stages of S. erinacei including immature egg, coracidium, plerocercoid in tadpole and rat, and adult. Proteolytic activity against carboxybenzoyl-phenylalanyl-arginyl-7-amino-4-methylcoumarin was detected in the extracts of coracidia and plerocercoid while no activity was observed in those of immature egg and adult. The specific activity in coracidial extracts was lower than that in the plerocercoid. Reverse transcription-polymerase chain reaction and Northern blot analysis demonstrated that the gene was expressed in the coracidium and plerocercoid but not in immature egg and adult. These results suggest that the 27 kDa cysteine protease is only expressed in the stages involving active migration of the parasite in the host tissue.

Kong, Yoon; Yun, Doo-Hee; Cho, Seung-Yull; Sohn, Woon-Mok; Chung, Young-Bae



Isolation and characterization of a 30 kDa protein with antifungal activity from leaves of Engelmannia pinnatifida.  

PubMed Central

During the course of screening plants for novel antifungal activity, we found that a high-molecular-mass fraction of an extract from leaves of Engelmannia pinnatifida exhibited potent and broad-spectrum antifungal activity. In this study a 30 kDa protein from E. pinnatifida leaves was purified to homogeneity by ammonium sulphate precipitation, gel filtration, Mono-Q and C18 reverse-phase column chromatographies. The purified protein showed potent antifungal activity against various plant pathogens with as little as 50 ng. The N-terminal amino acid sequence of the purified protein was determined as XXTKFDFFTLALQXPAXF, where X indicates an unidentified residue. This sequence showed 35-50% sequence identity with purified style glycoproteins associated with self-incompatibility from wild tomato, tobacco and petunia, a phosphate-starvation-induced ribonuclease from cultured tomato cells and the SIR 63.4 kDa protein from yeast.

Huynh, Q K; Borgmeyer, J R; Smith, C E; Bell, L D; Shah, D M



Fucoxanthin-chlorophyll proteins in diatoms: 18 and 19 kDa subunits assemble into different oligomeric states.  


Fucoxanthin-chlorophyll proteins were purified from the centric diatom Cyclotella meneghiniana. Two major fractions were observed that differed in their polypeptide composition and oligomeric state. Trimers consist of mainly 18 kDa polypeptides. Higher oligomers are tightly assembled from different trimers, which contain mostly 19 kDa subunits. In both oligomeric states, the excitation energy coupling between fucoxanthin and chlorophyll a was preserved, and chlorophyll c was shown to transfer energy efficiently to chlorophyll a. Circular dichroism spectra showed close interaction between fucoxanthin and chlorophyll a, and different chlorophyll a molecules were demonstrated to interact excitonically. The assembly of trimers of antenna proteins with a distinct subunit composition into higher oligomeric states was not reported so far and differs from the situation found in higher plants. The differences in the supramolecular structure of the fucoxanthin-chlorophyll proteins reflect the dissimilar arrangement of the thylakoid membranes in diatoms, which lack the grana-stroma distinction. PMID:14596618

Büchel, Claudia



The action of a dopamine (DA1) receptor agonist, fenoldopam in human vasculature in vivo and in vitro.  

PubMed Central

This study was designed to investigate dopaminergic mechanisms in human vasculature using the selective vascular dopamine receptor agonist fenoldopam in vivo and in vitro. In vivo, forearm blood flow was measured plethysmographically and in vitro isolated rings of human blood vessels from a variety of sites were used for tissue bath studies. Intra-arterial fenoldopam markedly increased forearm blood flow, this effect was antagonised by (R) sulpiride, a vascular dopamine (DA1) antagonist, but not by metoclopramide, a neuronal (DA2) antagonist, or by guanethidine, an adrenergic neurone blocking agent. In vitro, fenoldopam relaxed preconstricted human renal, mesenteric and lumbar arteries, but not saphenous vein in a concentration dependent manner. (RS) sulpiride and SCH 23390 competitively antagonised this effect. These studies demonstrate the presence of a vasodilatory vascular dopamine receptor in man both in vivo and in vitro.

Hughes, A; Thom, S; Martin, G; Redman, D; Hasan, S; Sever, P



Wave energy in white dwarf atmospheres. I - Magnetohydrodynamic energy spectra for homogeneous DB and layered DA stars  

NASA Technical Reports Server (NTRS)

The radiative damping of acoustic and MHD waves that propagate through white dwarf photospheric layers is studied, and other damping processes that may be important for the propagation of the MHD waves are calculated. The amount of energy remaining after the damping processes have occurred in different types of waves is estimated. The results show that lower acoustic fluxes should be expected in layered DA and homogeneous DB white dwarfs than had previously been estimated. Acoustic emission manifests itself in an enhancement of the quadrupole term, but this term may become comparable to or even lower than the dipole term for cool white dwarfs. Energy carried by the acoustic waves is significantly dissipated in deep photospheric layers, mainly because of radiative damping. Acoustically heated corona cannot exist around DA and DB white dwarfs in a range T(eff) = 10,000-30,000 K and for log g = 7 and 8. However, relatively hot and massive white dwarfs could be exceptions.

Musielak, Zdzislaw E.



[The "myologie dynamique" by Girolamo Fabrizi da Aquapendente in the scientific language in the Renaissance age (XVI-XVII)].  


Beginning from the XV century, mechanical materialism underwent an evolution in "biological mechanics" within the scientific doctrine. Among the greatest exponents of this new current there were two Italian men, Leonardo da Vinci (1452-1519) and Girolamo da Acquapendente (1533-1619). By the trend given by Leonardo, the myology, instead of being a static science, took a dynamic meaning and valence. Later, Fabrizi resumed and investigated the subject above all in its less known expression, elaborating an original theory. With Acquapendente, the anatomy lost its merely descriptive pecularity and evolved in analysis of the structure in connection with the function. Moreover, he opposed the syllogism against the mechanic language and the mathematical formulation. A new scientific way will be afterwards characterized by Galileo Galilei in the field of the physics and by Giovanni Alfonso Borrelli in the biology. PMID:11640090

Stroppiana, L



Why DA and DB white dwarfs do not show coronal activity and p-mode oscillations  

NASA Technical Reports Server (NTRS)

The problems of nonradiative heating of outer atmospheric layers and p-mode oscillations in white dwarfs caused by acoustic waves generated in convective zones are discussed. These effects have been studied by calculating the cutoff periods for adiabatic and isothermal waves propagating in atmospheres of DA and DB stars with Teff greater than or equal 20,000 K and log g = 6-9. The obtained cutoff periods are approximately bounded by 0.01 and 40 sec for high- and low-gravity white dwarfs, respectively. Expected amplitudes of p-mode oscillations corresponding to trapped acoustic waves with small angular wave numbers are estimated, indicating that the amplitudes could be observed as Doppler shifts of spectral lines which might be detectable if adequate spectral resolution were available. The luminosity variations corresponding to these amplitudes are unlikely to be observable when all damping processes are accounted for. Results also indicate that the present theory of convection predicts some irregularities in the behavior of physical parameters.

Musielak, Z. E.; Fontenla, J. M.



Involvement of androgen receptor and glucose-regulated protein 78kDa in human hepatocarcinogenesis.  


Previous studies demonstrated that androgen receptor (AR) is expressed in human hepatocellular carcinoma (HCC), one of the male-dominant diseases. Glucose-regulated protein 78kDa (GRP78/Bip), which has a role in cancer development, is one of the androgen response genes in prostate cell lines. The aim of this study was to investigate the impact of AR on endoplasmic reticulum (ER)-stress signaling in human hepatoma. AR and GRP78 expressions were examined in human liver tissue panels. Human hepatoma cells stably expressing short hairpin RNA targeting AR and cells over-expressing AR were generated. The expressions of ER-stress molecules and AR were measured by real-time RT-PCR and Western blotting. The effect of AR on ER-stress responsive gene expression was examined by reporter assay. Strong positive correlation between AR mRNA and GRP78 mRNA was observed in stage I/II-HCCs. AR enhanced ER-stress responsive element activities and GRP78 expression, and regulated ER-stress response in hepatocytes. Sorafenib strongly induced significant apoptosis in HepG2 cells by the inhibition of AR and inhibition of the downstream GRP78. AR seems a co-regulator of GRP78 especially in earlier-stage HCC. AR plays a critical role in controlling ER-stress, providing new therapeutic options against HCC. PMID:24583399

Jiang, Xia; Kanda, Tatsuo; Nakamoto, Shingo; Miyamura, Tatsuo; Wu, Shuang; Yokosuka, Osamu



Pupillometry and 123I-DaTSCAN imaging in Parkinson's disease: a comparison study.  


The purpose of this study was the evaluation of pupil light reflex (PLR) in patients with Parkinson's disease (PD) by using a modern pupillometry system and the investigation of its potential relationship with dopamine transporter imaging (DaTSCAN), which is an objective method for the evaluation of presynaptic dopaminergic system. PLR was evaluated using pupillometry in 35 patients with PD without clinical evidence of autonomic dysfunction and 44 healthy matched controls. PLR was elicited using a fully automated pupillometry system and six parameters were measured. Dopamine transporter imaging was performed using radioactive ioflupane (123)I-FP-CIT [(123)I-N-?-fluoropropyl-2?-carbomethoxy-3?-(4-iodophenyl)-nortropane]. A significant increase in latency and a significant decrease in amplitude, maximum constriction velocity, as well as maximum acceleration were observed in PD patients. There was no significant difference in initial radius and minimum radius values. Investigating the relationship between pupillometry parameters and (123)I-FP-CIT binding values, we correlated values from the semiquantitative analysis of radioligand uptake with pupillometry parameters, but we found no significant correlation. This study demonstrates PLR impairment in patients with PD without overt autonomic dysfunction. This impairment does not seem to correspond to the reduction of radioligand binding in the striatum as the result of presynaptic dopaminergic dysfunction, suggesting a different deterioration rate of these systems. PMID:21883027

Giza, Evangelia; Fotiou, Dimitrios; Bostantjopoulou, Sevasti; Katsarou, Zoe; Gerasimou, George; Gotzamani-Psarrakou, Anna; Karlovasitou, Anna



Preliminary study of kaonic deuterium X-rays by the SIDDHARTA experiment at DA?NE  

PubMed Central

The study of the K¯N system at very low energies plays a key role for the understanding of the strong interaction between hadrons in the strangeness sector. At the DA?NE electron–positron collider of Laboratori Nazionali di Frascati we studied kaonic atoms with Z=1 and Z=2, taking advantage of the low-energy charged kaons from ?-mesons decaying nearly at rest. The SIDDHARTA experiment used X-ray spectroscopy of the kaonic atoms to determine the transition yields and the strong interaction induced shift and width of the lowest experimentally accessible level (1s for H and D and 2p for He). Shift and width are connected to the real and imaginary part of the scattering length. To disentangle the isospin dependent scattering lengths of the antikaon–nucleon interaction, measurements of K?p and of K?d are needed. We report here on an exploratory deuterium measurement, from which a limit for the yield of the K-series transitions was derived: Y(Ktot)<0.0143 and Y(K?)<0.0039 (CL 90%). Also, the upcoming SIDDHARTA-2 kaonic deuterium experiment is introduced.

Bazzi, M.; Beer, G.; Berucci, C.; Bombelli, L.; Bragadireanu, A.M.; Cargnelli, M.; Curceanu (Petrascu), C.; d'Uffizi, A.; Fiorini, C.; Frizzi, T.; Ghio, F.; Guaraldo, C.; Hayano, R.; Iliescu, M.; Ishiwatari, T.; Iwasaki, M.; Kienle, P.; Levi Sandri, P.; Longoni, A.; Marton, J.; Okada, S.; Pietreanu, D.; Ponta, T.; Romero Vidal, A.; Sbardella, E.; Scordo, A.; Shi, H.; Sirghi, D.L.; Sirghi, F.; Tatsuno, H.; Tudorache, A.; Tudorache, V.; Vazquez Doce, O.; Widmann, E.; Zmeskal, J.



Why DA and DB white dwarfs do not show coronal activity and p-mode oscillations  

SciTech Connect

The problems of nonradiative heating of outer atmospheric layers and p-mode oscillations in white dwarfs caused by acoustic waves generated in convective zones are discussed. These effects have been studied by calculating the cutoff periods for adiabatic and isothermal waves propagating in atmospheres of DA and DB stars with Teff greater than or equal 20,000 K and log g = 6-9. The obtained cutoff periods are approximately bounded by 0.01 and 40 sec for high- and low-gravity white dwarfs, respectively. Expected amplitudes of p-mode oscillations corresponding to trapped acoustic waves with small angular wave numbers are estimated, indicating that the amplitudes could be observed as Doppler shifts of spectral lines which might be detectable if adequate spectral resolution were available. The luminosity variations corresponding to these amplitudes are unlikely to be observable when all damping processes are accounted for. Results also indicate that the present theory of convection predicts some irregularities in the behavior of physical parameters. 34 refs.

Musielak, Z.E.; Fontenla, J.M. (NASA, Marshall Space Flight Center, Huntsville, AL (USA))



Radiative levitation of silicon in the atmospheres of two Hyades DA white dwarfs  

NASA Astrophysics Data System (ADS)

The presence of silicon at the surface of the two Hyades DA white dwarfs WD 0421+162 and WD 0431+126 requires mechanisms that counteract the effects of the downward diffusion. Radiative levitation calculations indicate that the silicon abundance observed in WD 0431+126 corresponds to the abundance supported by radiative levitation. Detailed time-dependent diffusion calculations that take into account radiative levitation and accretion indicate that accretion with rates of dot{M}_{Si} ? 10^4 g s-1 could also be present without disrupting the abundance supported by radiative levitation. In the case of WD 0421+162, accretion with a rate of dot{M}_{Si} = 10^{5.1} g s-1 must be invoked, because its observed silicon abundance is larger than the abundance supported by radiative levitation. This accretion rate is lower than the accretion rate given by the accretion-diffusion model, because the radiative levitation slows down the downward diffusion of silicon. The silicon abundances observed in the two Hyades white dwarfs cannot be interpreted solely in terms of accretion. The interpretation of the silicon abundances must take into account the interplay between radiative levitation and accretion.

Chayer, P.



Chimeric Avidin - NMR Structure and Dynamics of a 56 kDa Homotetrameric Thermostable Protein  

PubMed Central

Chimeric avidin (ChiAVD) is a product of rational protein engineering remarkably resistant to heat and harsh conditions. In quest of the fundamentals behind factors affecting stability we have elucidated the solution NMR spectroscopic structure of the biotin–bound form of ChiAVD and characterized the protein dynamics through 15N relaxation and hydrogen/deuterium (H/D) exchange of this and the biotin–free form. To surmount the challenges arising from the very large size of the protein for NMR spectroscopy, we took advantage of its high thermostability. Conventional triple resonance experiments for fully protonated proteins combined with methyl–detection optimized experiments acquired at 58°C were adequate for the structure determination of this 56 kDa protein. The model–free parameters derived from the 15N relaxation data reveal a remarkably rigid protein at 58°C in both the biotin–bound and the free forms. The H/D exchange experiments indicate a notable increase in hydrogen protection upon biotin binding.

Tossavainen, Helena; Kukkurainen, Sampo; Maatta, Juha A. E.; Kahkonen, Niklas; Pihlajamaa, Tero; Hytonen, Vesa P.; Kulomaa, Markku S.; Permi, Perttu



Regulation of Translocator Protein 18 kDa (TSPO) Expression in Health and Disease States  

PubMed Central

Translocator protein (TSPO) is an 18-kDa high affinity cholesterol- and drug-binding protein found primarily in the outer mitochondrial membrane. Although TSPO is found in many tissue types, it is expressed at the highest levels under normal conditions in tissues that synthesize steroids. TSPO has been associated with cholesterol import into mitochondria, a key function in steroidogenesis, and directly or indirectly with multiple other cellular functions including apoptosis, cell proliferation, differentiation, anion transport, porphyrin transport, heme synthesis, and regulation of mitochondrial function. Aberrant expression of TSPO has been linked to multiple diseases, including cancer, brain injury, neurodegeneration, and ischemia reperfusion injury. There has been an effort during the last decade to understand the mechanisms regulating tissue- and disease-specific TSPO expression and to identify pharmacological means to control its expression. This review focuses on the current knowledge regarding the chemicals, hormones, and molecular mechanisms regulating Tspo gene expression under physiological conditions in a tissue- and disease-specific manner. The results described here provide evidence that the PKC?-ERK1/2-AP1/Stat3 signal transduction pathway is the primary regulator of Tspo gene expression in normal and pathological tissues expressing high levels of TSPO.

Batarseh, Amani; Papadopoulos, Vassilios



Structural Basis for the Catalytic Mechanism of Mammalian 25-kDa Thiamine Triphosphatase*S?  

PubMed Central

Mammalian soluble thiamine triphosphatase (ThTPase) is a 25-kDa cytosolic enzyme that specifically catalyzes the conversion of thiamine triphosphate (ThTP) to thiamine diphosphate and has an absolute requirement for divalent cations. We have investigated the kinetic properties of recombinant mouse thiamine triphosphatase (mThTPase) and determined its solution structure by NMR spectroscopy. Residues responsible for binding Mg2+ and ThTP were determined from NMR titration experiments. The binding of Mg2+ induced only a minor local conformational change, whereas ThTP binding was found to cause a more global conformational change. We derived a structural model for the mThTPase·ThTP·Mg2+ ternary complex and concluded from this that whereas free mThTPase has an open cleft fold, the enzyme in the ternary complex adopts a tunnel fold. Our results provide a functional rationale for a number of conserved residues and suggest an essential role for Mg2+ in catalysis. We propose a mechanism underlying the high substrate specificity of mThTPase and discuss the possible role of water molecules in enzymatic catalysis.

Song, Jikui; Bettendorff, Lucien; Tonelli, Marco; Markley, John L.



Distinct sources for syntectonic Variscan granitoids: Insights from the Aguiar da Beira region, Central Portugal  

NASA Astrophysics Data System (ADS)

The Variscan syntectonic granitoid plutons from the Aguiar da Beira region (central Portugal) were emplaced into metasediments of Late Proterozoic-Early Cambrian age during the last Variscan ductile tectonic event (D3), which is related to dextral and sinistral shearing. The older intrusion, with a U-Pb ID-TIMS zircon age of 321.8 ± 2.0 Ma, consists of porphyritic biotite granodiorite-granite with transitional I-S type geochemical signature, relatively low 87Sr/86Sr322 ratios (0.7070-0.7074), ?Nd322 values of - 3.9 to - 4.6 and whole-rock and zircon ?18O values of 10.6‰ and 8.0‰, respectively. By contrast, the younger intrusion is an S-type muscovite-biotite leucogranite, emplaced at 317.0 ± 1.1 Ma, showing more radiogenic 87Sr/86Sr317 = 0.7104-0.7146, lower ?Nd317 values of - 7.7 to - 8.7 and higher ?18O-wr = 11.3‰ and ?18O-zr = 9.5‰. The combined isotopic and geochemical evidence supports a lower crustal origin for the biotite granodiorite-granite, involving the anatexis of lower crustal metaigneous protoliths, and possible hybridization with mantle-derived magmas. A shallower origin, at mid crustal levels, from pure crustal derivation, through moderate degrees of partial melting of Proterozoic-Cambrian metasediments is proposed instead for the muscovite-biotite leucogranite.

Costa, M. M.; Neiva, A. M. R.; Azevedo, M. R.; Corfu, F.



ROSAT Studies of the Composition and Structure of DA White Dwarf Atmospheres  

NASA Astrophysics Data System (ADS)

We present here a detailed study of a sample of hot I)A white dwarfs detected in the EUV and soft X-ray bands by ROSAT during its all-sky survey. The survey data are combined with additional ROSAT pointed-phase observations, earlier EXOSAT results and the available optically determined effective temperatures and gravities. We show that the spectra of those stars with temperatures below ?40 000 K are well described by nearly pure H atmospheres, with only very small traces of additional opacity due to He allowed by the data. Above ?40 000 K, however, neither homogeneous nor stratified H + He models can, in general, explain the observed EUV and X-ray fluxes. We conclude that additional sources of opacity in the form of trace metals must be present in the photospheres of these stars. The disappearance of this material when the stars cool below 40 000-50 000 K is in agreement with theoretical radiative levitation calculations. We propose that these results are direct observational evidence that the composition of most DA white dwarf atmospheres is dominated by the balance between gravitational and radiative forces. Limits on the H-layer mass obtained for the cooler stars indicate that He makes only a minimal, if any, contribution to the EUV/soft X-ray opacity in the phosphere.

Barstow, M. A.; Fleming, T. A.; Diamond, C. J.; Finley, D. S.; Sansom, A. E.; Rosen, S. R.; Koester, D.; Holberg, M. C.; Marsh, J. B.; Kidder, K.



Introduction to Garudapur?na with reference to Ayurv?da.  


The Pur?nas are the encyclopedic works of the ancient and medieval Hindu religion, philosophy, history, politics, ethics, sciences etc. There are 18 (Ast?da?a) pur?nas, which are, considered as mah?pur?nas, among which Garudapur?na is popular one. The Garudapur?na is divided into two parts viz., P?rvakhanda and Uttarakhanda. The first part, which is also called Ac?rakhanda consists of 240 chapters. The greater part of the P?rvakhanda occupies the descriptions of Vratas (religious observances), sacred places dedicated to the S?rya (sun), Lord Siva and Lord Visnu. It also contains treatises on various aspects like astrology, palmistry, politics, S?nkhya, Y?ga, anatomy, precious stones and extensive information on vedic medicine i.e., Ayurveda. The Uttarakhanda consists of two khandas viz. Dharmakhanda and Brahmakhanda, which are divided into 42 and 29 chapters, respectively. The Dharmakhanda is also known as the Pr?takalpa which contains directions for the performance of obsequies rites. The Pr?takalpa portion of the Garudapur?na is generally recited during the period of mourning and so its importance is self-evident. It is almost impossible to narrate within such a small framework, the wide range of splendid truths scattered in the pages of this noble pur?nam. Little information is available from internal evidence to establish its exact period. However, it is supposed to be quite ancient in its origin. PMID:18175646

Subhose, Varanasi; Narayana, Ala; Prasad, P V V; Rao, M Mruthyumjaya



Translocator Protein 18 kDa Negatively Regulates Inflammation in Microglia.  


Translocator protein 18 kDa (TSPO) is a mitochondrial outer membrane protein. Although TSPO expression is up-regulated during neuroinflammation, the role of TSPO and its signaling mechanisms in regulation of neuroinflammation remains to be elucidated at the molecular level. Here we demonstrate that TSPO is a negative regulator of neuroinflammation in microglia. Over-expression of TSPO decreased production of pro-inflammatory cytokines upon lipopolysaccharide treatment while TSPO knock-down had the opposite effect. Anti-inflammatory activity of TSPO is also supported by increased expression of alternatively activated M2 stage-related genes. These data suggest that up-regulation of TSPO level during neuroinflammation may be an adaptive response mechanism. We also provide the evidence that the repressive activity of TSPO is at least partially mediated by the attenuation of NF-?B activation. Neurodegenerative diseases are characterized by loss of specific subsets of neurons at the particular anatomical regions of the central nervous system. Cause of neuronal death is still largely unknown, but it is becoming clear that neuroinflammation plays a significant role in the pathophysiology of neurodegenerative diseases. Understanding the mechanisms underlying the inhibitory effects of TSPO on neuroinflammation can contribute to the therapeutic design for neurodegenerative diseases. PMID:24687172

Bae, Keun-Ryung; Shim, Hyun-Jung; Balu, Deebika; Kim, Sang Ryong; Yu, Seong-Woon



[The history of prostate cancer from the beginning to DaVinci].  


For hardly any other organ can the development of medicine and technical advances in the last 150 years be so clearly illustrated as for the prostate. The history of radical prostatectomy was initially characterised by the problems in approaching this relatively difficulty accessible organ. In 1867, Theodor Billroth in Vienna performed the first partial prostatectomy via a perineal access. In 1904, Hugh Hampton Young and William Stewart Halsted at the Johns Hopkins Hospital in Baltimore / USA carried out the first successful extracapsular perineal prostatectomy and opened up a new era. In Germany, Prof. Friedrich Voelcker in Halle in 1924 developed the so-called ischiorectal prostatectomy. But it was left to Terence Millin to publish in 1945 the first series of retropubic prostatectomies. In 1952, the sacroperineal approach according to Thiermann and the sacral prostatectomy according to were introduced. Finally, in 1991 another new era in prostate surgery started with the first laparoscopic prostatectomy. This development peaked in 2011 with the presentation of the laparoscopic DaVinci prostatectomy by Binder. Originally a stepchild of urological surgery that was to be avoided whenever possible due to the fear of serious complications, the prostate has progressed in the course of time to an obscure object of lust. The stepchild has become the favorite child. PMID:23035261

Hatzinger, M; Hubmann, R; Moll, F; Sohn, M



Translocator protein (18 kDa) TSPO: an emerging therapeutic target in neurotrauma  

PubMed Central

Traumatic brain injury (TBI) induces physical, cognitive, and psychosocial deficits that affect millions of patients. TBI activates numerous cellular mechanisms and molecular cascades that produce detrimental outcomes, including neuronal death and loss of function. The mitochondrion is one of the major targets of TBI, as seen by increased mitochondrial activity in activated and proliferating microglia (due to high energy requirements and/or calcium overload) as well as increased reactive oxygen species, changes in mitochondrial permeability transition, release of cytochrome c, caspase activation, reduced ATP levels, and cell death in neurons. Translocator protein (TSPO) is an 18-kDa outer mitochondrial membrane protein that interacts with the mitochondria permeability transition pore and binds with high affinity to cholesterol and various classes of drug ligands, including some benzodiazepines such as 4?-chlorodiazepam (Ro5-4864). Although TSPO levels in the brain are low, they are increased after brain injury and inflammation. This finding has led to the proposed use of TSPO expression as a marker of brain injury and repair. TSPO drug ligands have been shown to participate in the control of mitochondrial respiration and function, mitochondrial steroid and neurosteroid formation, as well as apoptosis. This review and commentary will outline our current knowledge of the benefits of targeting TSPO for TBI treatment and the mechanisms underlying the neuroprotective effects of TSPO drug ligands in neurotrauma.

Papadopoulos, Vassilios; Lecanu, Laurent



Role of mitochondrial translocator protein (18 kDa) on mitochondrial- related cell death processes.  


The 18 kDa translocator protein (TSPO) is able to modulate several mitochondria-related cell death processes due to close association with proteins and other molecules involved to the mitochondrial permeability transition pore. In this way, herein we review cell death mechanisms targeting mitochondria, including programmed cell death type I, II and III. Several proteins involved in these cell death processes and with a possible interplay with the TSPO are also discussed including the voltage dependent anion channel, the adenine nucleotide transporter, cardiolipin, and the Bcl-2 family proteins. Noteworthy, TSPO has been also implicated in various other functions including mitochondrial respiration, immune and phagocytic host-defense response, microglial activation, inflammation, cell growth and differentiation, cancer, cell proliferation, ischemia, and mental and neuropathological disorders. We focused in recent studies of the TSPO particularly on cancer and neurodegeneration, thus presenting the TSPO as a core element in the role of mitochondria in diseases and related processes. Clinical benefit may be attainable by increasing pharmacological knowledge related to the TSPO. Recent patents typically relate to diagnosis and treatment of TSPO-related pathological conditions including cancer, and inflammatory conditions, as well as disorders associated with central nervous system, such as neurodegeneration, convulsions, anxiety, mental disorders, and dementia. PMID:23368281

Caballero, Beatriz; Veenman, Leo; Gavish, Moshe



Third harmonic cavity design and RF measurements for the Frascati DA?NE collider  

NASA Astrophysics Data System (ADS)

Third harmonic passive RF cavities have been proposed for installation in both rings of the DA?NE factory collider to improve the Touschek lifetime and to increase the Landau damping. This paper illustrates the design of the harmonic cavities. The main requirements were to obtain a relatively low R/Q factor and a quality factor Q as high as possible to satisfy beam dynamics requirements and to damp all the higher order mode (HOM) to a harmless level in order to avoid multibunch instabilities. A spherical shape of the cavity central body has been chosen as an optimum compromise between a high Q resonator and a low R/Q factor. HOM suppression has been provided by a ferrite ring damper designed for the superconducting cavities of the high energy ring of the KEK-B factory. The design and electromagnetic properties of the resonant modes have been studied with MAFIA and HFSS codes. Cavities have been made of aluminum and the RF measurements have been performed to characterize them. The measurements are in a good agreement with numerical simulations results, demonstrating a satisfactory HOM damping.

Alesini, David; Boni, Roberto; Gallo, Alessandro; Marcellini, Fabio; Zobov, Mikhail; Migliorati, Mauro; Palumbo, Luigi



Swift XRT Detection of Supernova 2010da in X-Rays  

NASA Astrophysics Data System (ADS)

Using Swift X-Ray Telescope (XRT) observations of SN 2010da in NGC 300 (CBET #2289, ATel #2632, 2633, 2636, 2637, 2638), obtained on 2010-05-24.7 (4 ks exposure time), simultaneous to the Swift Ultraviolet/Optical Telescope data (ATel #2633), a bright X-ray point-source is detected, consistent with the optical position of the SN, with a 10-sigma significance of source detection. The PSF, dead-time, and vignetting corrected XRT net count rate is (3.0+/-0.3)E-02 cts/s, corresponding to an unabsorbed (0.2-10 keV band) X-ray flux of (1.4+/-0.1)E-12 erg/cm/cm/s and a luminosity of (6.0+/-0.6)E38 erg/s for an adopted thermal plasma spectrum with a temperature of kT = 10 keV, a Galactic foreground column density of N_H = 3.23E+20 (Dickey & Lockman, 1990, ARAA 28) and a distance of 1.9 Mpc (NED).

Immler, S.; Brown, P.; Russell, B. R.



Tumor marker nucleoporin 88 kDa regulates nucleocytoplasmic transport of NF-{kappa}B  

SciTech Connect

Nucleoporin 88 kDa (Nup88) is a tumor marker, overexpressed in various types of cancer. In Drosophila Nup88 (mbo) was reported to selectively mediate the nucleocytoplasmic transport of NF-{kappa}B, an ubiquitous transcription factor involved in immune responses, apoptosis, and cancer. We addressed the function of Nup88 in mammalian cells. Selective depletion of Nup88 by small interfering RNA (siRNA) inhibited NF-{kappa}B-dependent reporter gene activation and the nuclear translocation of NF-{kappa}B without affecting the upstream activation pathway in NIH3T3 cells. In contrast, nuclear translocation of glucocorticoid receptor was not reduced by the depletion of Nup88. In metastatic melanoma cells overexpressing Nup88, constitutive activation of NF-{kappa}B was found both in nucleus and cytoplasm. Nup88 depletion in these cells reduced TNF-induced nuclear accumulation of NF-{kappa}B subunits. We conclude that Nup88 regulates the activity of NF-{kappa}B at the level of nucleocytoplasmic transport. Overexpression of Nup88 in tumor cells may, thus be involved in the constitutive NF-{kappa}B activation.

Takahashi, Nozomi [Rheumatology Research and Advanced Therapeutics, 272, NCMLS, Radboud University Medical Centre Nijmegen, P.O. Box 9101, Geert Grooteplein 26-28, 6500 HB Nijmegen (Netherlands)], E-mail:; Kilsdonk, Jeroen W.J. van [Department of Biomolecular Chemistry, IMM and NCMLS, Radboud University Nijmegen, 6500 HB Nijmegen (Netherlands); Ostendorf, Benedikt [Rheumatology Research and Advanced Therapeutics, 272, NCMLS, Radboud University Medical Centre Nijmegen, P.O. Box 9101, Geert Grooteplein 26-28, 6500 HB Nijmegen (Netherlands); Centre for Rheumatology, Department of Endocrinology, Diabetology and Rheumatology, Heinrich-Heine University of Duesseldorf, 40225 Duesseldorf (Germany); Smeets, Ruben [Rheumatology Research and Advanced Therapeutics, 272, NCMLS, Radboud University Medical Centre Nijmegen, P.O. Box 9101, Geert Grooteplein 26-28, 6500 HB Nijmegen (Netherlands); Bruggeman, Sophia W.M. [Department of Biomolecular Chemistry, IMM and NCMLS, Radboud University Nijmegen, 6500 HB Nijmegen (Netherlands); Netherlands Cancer Institute, 1066CX Amsterdam (Netherlands); Alonso, Angel [Deutsches Krebsforschungszentrum (DKFZ) Heidelberg, 69120 Heidelberg (Germany); Loo, Fons van de [Rheumatology Research and Advanced Therapeutics, 272, NCMLS, Radboud University Medical Centre Nijmegen, P.O. Box 9101, Geert Grooteplein 26-28, 6500 HB Nijmegen (Netherlands); Schneider, Matthias [Centre for Rheumatology, Department of Endocrinology, Diabetology and Rheumatology, Heinrich-Heine University of Duesseldorf, 40225 Duesseldorf (Germany); Berg, Wim B. van den [Rheumatology Research and Advanced Therapeutics, 272, NCMLS, Radboud University Medical Centre Nijmegen, P.O. Box 9101, Geert Grooteplein 26-28, 6500 HB Nijmegen (Netherlands); Swart, Guido W.M. [Department of Biomolecular Chemistry, IMM and NCMLS, Radboud University Nijmegen, 6500 HB Nijmegen (Netherlands)



Evidence for an external origin of heavy elements in hot DA white dwarfs  

NASA Astrophysics Data System (ADS)

We present a series of systematic abundance measurements for 89 hydrogen atmosphere (DA-type) white dwarfs with temperatures spanning 16 000-77 000 K drawn from the FUSE spectral archive. This is the largest study to date of white dwarfs where radiative forces are significant, exceeding our earlier work, based mainly on International Ultraviolet Explorer and Hubble Space Telescope data, by a factor 3. Using heavy element blanketed non-local thermodynamic equilibrium stellar atmosphere calculations, we have addressed the heavy element abundance patterns making completely objective measurements of abundance values and their error ranges using a ?2 fitting technique. We are able to establish the broad range of abundances seen in a given temperature range and establish the incidence of stars which appear, in the optical, to be atmospherically devoid of any material other than H. We compare the observed abundances to predictions of radiative levitation calculations, revealing little agreement. We propose that the supply of heavy elements is accreted from external sources rather than being intrinsic to the star. These elements are then retained in the white dwarf atmospheres by radiative levitation, a model that can explain both the diversity of measured abundances for stars of similar temperature and gravity, including cases with apparently pure H envelopes, and the presence of photospheric metals at temperatures where radiative levitation is no longer effective.

Barstow, M. A.; Barstow, J. K.; Casewell, S. L.; Holberg, J. B.; Hubeny, I.



Biosynthesis of polyunsaturated fatty acids in the oleaginous marine diatom Fistulifera sp. strain JPCC DA0580.  


Studies of polyunsaturated fatty acid (PUFA) biosynthesis in microalgae are of great importance for many reasons, including the production of biofuel and variable omega 3-long chain PUFAs. The elucidation of the PUFA biosynthesis pathway is necessary for bioengineering to increase or decrease PUFA content in certain microalgae. In this study, we identified the PUFA synthesis pathway in the oleaginous marine diatom, Fistulifera sp. strain JPCC DA0580, a promising candidate for biodiesel production. The data revealed not only the presence of the desaturases and elongases involved in eicosapentaenoic acid (EPA) synthesis, but also the unexpected localization of ?3-desaturase expression in the chloroplast. This suggests that this microalga might perform the final step of EPA synthesis in the chloroplast and not in the endoplasmic reticulum (ER) like other diatoms. The detailed fatty acid profile suggests that the EPA was synthesized only through the ?6-pathway in this strain, which was also different from other diatoms. Finally, the transcriptome analysis demonstrated an overall down-regulation of desaturases and elongases over incubation time. These genetic features might explain the decrease of PUFA percentage over incubation time in this strain. The important insights into metabolite synthesis acquired here will be useful for future metabolic engineering to control PUFA content in this diatom. PMID:24335525

Liang, Yue; Maeda, Yoshiaki; Sunaga, Yoshihiko; Muto, Masaki; Matsumoto, Mitsufumi; Yoshino, Tomoko; Tanaka, Tsuyoshi



Emerging roles for the Ro 60 kDa autoantigen in noncoding RNA metabolism  

PubMed Central

All cells contain an enormous variety of ribonucleoprotein complexes that function in diverse processes. Although the mechanisms by which many of these RNPs contribute to cell metabolism are well understood, the roles of others are only now beginning to be revealed. A member of this latter category, the Ro 60 kDa protein and its associated noncoding Y RNAs, was discovered because the protein component is a frequent target of the autoimmune response in patients with the rheumatic diseases systemic lupus erythematosus and Sjögren’s syndrome. Recent studies have shown that Ro is ring-shaped, binds the single-stranded ends of misfolded noncoding RNAs in its central cavity, and may function in noncoding RNA quality control. Although Ro is not present in yeast, many bacterial genomes contain potential Ro orthologs. In the radiation-resistant eubacterium Deinococcus radiodurans, the Ro ortholog functions with exoribonucleases during stress-induced changes in RNA metabolism. Moreover, in both D. radiodurans and animal cells, Ro is involved in the response to multiple types of environmental stress. Finally, Y RNAs can influence the subcellular location of Ro, inhibit access of the central cavity to other RNAs and may also act as binding sites for proteins that influence Ro function.

Sim, Soyeong; Wolin, Sandra L.



[Morbidity in Chagas' disease. III. Longitudinal study of 6 years, in Virgem da Lapa, MG, Brazil].  


In a clinical, radiological and electrocardiographical, follow-up study of the "case control" type performed in Virgem da Lapa, Minas Gerais State, Brazil, 124 chagasic patients were followed during six years. The results of the patients, the majority in the indeterminate form, did not register any change, in 32.2% there was a progress in the disease and in 5.6% the electrocardiogram returned to normal. These results when compared to that achieved by the control group, composed of pairs of non chagasic persons with the same age and sex, was shown to be 27.4% higher than among patients with positive serology. This factor represents the excess risk or exclusively chagasic component in the development of the disease. No differences were observed by sex related to the development of the disease. It was more premature and seven times more frequent however when related to the cardiopathy than to the megaesophagus. Both conditions occurring mainly in slight or moderate degree. In 192 chagasic patients and 188 non chagasic persons observed in that area in the same period, the mortality was 3.6 times higher among the chagasic patients with a letality due to cardiopathy of 8.9% without difference between sexes but more premature among the males. Sudden death was more frequent than that one caused by cardiac insufficiency. The prognostic was good for the patients with indeterminate and digestive forms and reserved for patients with the highest degree of cardiopathy. PMID:3937014

Pereira, J B; Willcox, H P; Coura, J R



Hormonal responses during prolonged exercise are influenced by a selective DA/NA reuptake inhibitor  

PubMed Central

Objective: A decrease in dopamine activity is thought to lead to a reduction in motivation and arousal and therefore to the "central" component of fatigue. The purpose of the present study was to investigate the effects of a dopamine (DA) noradrenaline (NA) reuptake inhibitor, bupropion (ZybanTM), on exercise performance and on the hormonal response to exercise. Methods: Eight healthy well trained male cyclists (Wattmax 397±15 W) participated in the study. Subjects completed one maximal exercise test (to determine maximal power output Wattmax), and two endurance performance tests (time trials) in a double blind randomised cross-over design. Subjects took either placebo capsules (lactose) or 2x300 mg bupropion (BUP). Blood samples were collected for adrenocorticotropin (ACTH), prolactin, cortisol, growth hormone, beta-endorphins, and catecholamines. Results: Performance was not influenced by BUP (placebo: 89±1 min; BUP 2x300 mg: 89±0.7 min). All hormones increased during exercise in all trials. Cortisol plasma concentrations were significantly higher in the BUP trial at rest, at min 60, and at the end of exercise, while beta-endorphins were higher in the BUP trial at the end of exercise and during recovery, and ACTH at the end of exercise. Conclusion: From the present results, we can conclude that bupropion had a more marked central noradrenergic effect (compared to dopaminergic) on the hormonal response to exercise, but no effect on the outcome of performance.

Piacentini, M; Meeusen, R; Buyse, L; De Schutter, G; De Meirleir, K



The origin of finches on Tristan da Cunha and Gough Island, central South Atlantic ocean.  


The Nesospiza finches of the Tristan da Cunha archipelago and Rowettia goughensis from Gough Island, 380 km distant, are both derived from tanager-finches (Thraupidae) that colonized the islands by crossing more than 3000 km of ocean from South America. Sequences from two mitochondrial and four nuclear genes indicate that the Patagonian bridled finches Melanodera are the closest relatives of the South Atlantic finches. Melanodera typically was sister to Rowettia, although some genes linked it more closely to Nesospiza. There was no evidence that Rowettia and Nesospiza are sister taxa, suggesting that the South Atlantic finches evolved from separate colonization events, as apparently was the case for moorhens Gallinula spp. at the two island groups. Genetic divergence between the two island finch genera thus provides an estimate of the maximum period of time they have been present at the islands, some 3-5 million years. A brief review of colonization histories suggests that island hopping by passerine birds is infrequent among islands more than 100-200 km apart. PMID:23751898

Ryan, Peter G; Klicka, Luke B; Barker, Keith F; Burns, Kevin J



Purification and characterization of a 100 kDa DNA polymerase from cauliflower inflorescence.  


A DNA polymerase from cauliflower (Brassica oleracea var. botrytis) inflorescence has been purified to near homogeneity through five successive column chromatographies, and temporally designated cauliflower polymerase 1. Cauliflower polymerase 1 is a monopolypeptide with a molecular mass of 100 kDa. The enzyme efficiently uses synthetic DNA homopolymers and moderately activated DNA and a synthetic RNA homopolymer as template-primers. The enzyme is strongly sensitive to dideoxythymidine triphosphate and N-ethylmaleimide, but it is insensitive to aphidicolin. It was stimulated with 250 mM KCl. Its mode of DNA synthesis is high-processive with or without proliferating-cell nuclear antigen. A 3'-->5' exonuclease activity is associated with cauliflower polymerase 1. The enzyme is clearly different from cauliflower mitochondrial polymerase and does not resemble the four different types of wheat DNA polymerase, designated wheat DNA polymerases A, B, CI and CII. In the present paper the role of the enzyme in plant DNA synthesis is discussed. PMID:9601087

Seto, H; Hatanaka, M; Kimura, S; Oshige, M; Tsuya, Y; Mizushina, Y; Sawado, T; Aoyagi, N; Matsumoto, T; Hashimoto, J; Sakaguchi, K



Identification of a 170-kDa protein over-expressed in lung cancers  

PubMed Central

Lung cancer is the leading cause for cancer death in both male and female populations. Although many molecular markers for lung cancer have been developed and useful for early detection of lung cancer, their function remains unknown. In this paper, we report our findings that a 170-kDa protein (p170) is over-expressed in all types of human lung cancers compared with normal tissues and it is identified as a subunit of translation initiation factor eIF3 by cDNA cloning. Translation initiation factors are a family of proteins that promote the initiation step of protein synthesis and are regulators of cell growth at the translational level. Further studies showed that p170?mRNA is ubiquitously expressed with higher levels in adult proliferating tissues (e.g. bone marrow) and tissues during development (e.g. fetal tissues). This study suggests that p170 and eIF3 may be important factors for cell growth, development, and tumorigenesis. © 2001 Cancer Research Campaign

Pincheira, R; Chen, Q; Zhang, J-T