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1

COMMUNAL SUCKLING IN WATER BUFFALO (Bubalus bubalis)  

Microsoft Academic Search

Murphey, R. M.; Paranhos da Costa, M. J. R.; Lima, L. O. S. and Duarte, F. A. M., 1991. Communal suckling in water buffalo (Bubalus bubalis). Appl. Anim. Behav. Sci., 28: 341-352 Communal nursing (an adult female allowing the offspring of another conspecific female to suckle) is a relatively frequent behavior in water buffalo (Bubalus bubalis). Fourteen lactating water buffalo

R. M. MURPHEY; SOUZA LIMA; MOURA DUARTE; Mateus J. R. Paranhos da Costa

2

Isolation of Arcobacter species in water buffaloes (Bubalus bubalis).  

PubMed

This is the first report of Arcobacter spp. in rectal fecal samples from healthy water buffaloes (Bubalus bubalis) reared on a dairy farm. Arcobacter species were isolated after enrichment, and isolates were identified at species level by multiplex-polymerase chain reaction assay. Thirty samples were examined and Arcobacter spp. were isolated from 96.7% of water buffaloes tested: 38 Arcobacter spp. isolates were obtained, with A. cryaerophilus as the dominant species followed by A. butzleri and A. skirrowii. Nine animals (31%) were colonized by more than one Arcobacter species. The present study indicates that water buffaloes can harbor a variety of Arcobacter spp. and that healthy buffaloes may act as hosts. Water buffalo fecal shedding of Arcobacter spp. may be of significance to human health, considering the potential fecal contamination during harvesting of raw milk and slaughtering. PMID:23536981

Piva, Silvia; Serraino, Andrea; Florio, Daniela; Giacometti, Federica; Pasquali, Frederique; Manfreda, Gerardo; Zanoni, Renato Giulio

2013-05-01

3

Genetic Resistance to Brucella abortus in the Water Buffalo (Bubalus bubalis)  

Microsoft Academic Search

Brucellosis is a costly disease of water buffaloes (Bubalus bubalis). Latent infections and prolonged incuba- tion of the pathogen limit the efficacy of programs based on the eradication of infected animals. We exploited genetic selection for disease resistance as an approach to the control of water buffalo brucellosis. We tested 231 water buffalo cows for the presence of anti-Brucella abortus

Giorgia Borriello; Rosanna Capparelli; Michele Bianco; Domenico Fenizia; Flora Alfano; Federico Capuano; Danilo Ercolini; Antonio Parisi; Sante Roperto; Domenico Iannelli

2006-01-01

4

The C-banding pattern of the Egyptian Water Buffalo (Bubalus bubalis)  

E-print Network

Note The C-banding pattern of the Egyptian Water Buffalo (Bubalus bubalis) E. P. CRIBIU A. OBEIDAH GIZA Egypt Summary The diploid chromosome number of the Egyptian Water Buffalo is 50 of which 10 heterochromatin. The karyotype of the Egyptian Water Buffalo (Bifbalus bubalis) has been reported by DE HONDT

Paris-Sud XI, Université de

5

Leptospirosis in water buffalo (Bubalus bubalis) in Trinidad  

E-print Network

The seroprevalence of leptospirosis in water buffalo (Bubalus bubalis) reared for meat in semi-intensive and extensive managed farms in Trinidad was determined. All sera were tested for specific antibodies against 17 internationally recognized serovars of Leptospira using the microscopic agglutination test (MAT). Animals with titres greater or equal to 100 were considered as seropositive indicating exposure to Leptospira and those with titres greater or equal to 800 were interpreted as cases of acute leptospirosis. Of a total of 226 apparently healthy water buffalo from five major farms in Trinidad tested, 33 (14.6%) were seropositive with titres ranging from 100 to 400. Three (60.0%) of 5 farms had seropositive animals with seropositivity rates ranging from 2.0 % (1 of 50) on Farm A to 32.7 % (16 of 49) on Farm B. The difference was statistically significant (P0.05; X2) effect on infection rate. The prevalent antibodies to serovars of Leptospira were farm-specific with specific antibodies to serovars Copenhageni and Georgia being predominant on Farm B having been detected in 10 (62.5%) and 9 (56.3%) respectively of 16 seropositive animals. On Farm D however, also with 16 seropositive animals, specific antibodies to serovars Patoc and Bratislava were most frequently detected, found in 11 (68.8%) and 5 (31.3%) respectively of seropositive animals. This is the first documentation of leptospirosis in water buffalo in the Caribbean region and the health risk posed to farm

Abiodun Adewale Adesiyun; Carol Hull-jackson; Nicole Clarke; C. Hull-jackson; N. Clarke; C. Whittington

6

Seroprevalence and correlates of Toxoplasma gondii infection in water buffaloes (Bubalus bubalis) in Veracruz state, Mexico  

Technology Transfer Automated Retrieval System (TEKTRAN)

Background: Infection with Toxoplasma gondii in water buffaloes (Bubalus bubalis) is of epidemiological importance because of the risk for transmission to humans. We sought to determine the seroprevalence of T. gondii infection in 339 water buffaloes in Veracruz State, Mexico using the modified aggl...

7

Analysis of mitochondrial D-loop region casts new light on domestic water buffalo ( Bubalus bubalis) phylogeny  

Microsoft Academic Search

The phylogeny of water buffaloes (Bubalus bubalis) is still a matter of discussion, especially if the two types of domestic water buffalo (swamp and river) derived from different domestication events or if they are products of human selection. To obtain more insight, we analyzed the entire mitochondrial D-loop region of 80 water buffaloes of four different breeds, i.e., 19 swamp

Gerold Kierstein; Marcelo Vallinoto; Artur Silva; Maria Paula Schneider; Leopoldo Iannuzzi; Bertram Brenig

2004-01-01

8

Prevalence, biology, and distribution pattern of Sarcocystis infection in water buffalo ( Bubalus bubalis ) in Iran  

Microsoft Academic Search

This study was conducted to determine the prevalence, distribution pattern, and the Sarcocystis species involved in slaughtered water buffaloes (Bubalus bubalis) in the Khuzestan, Iran by macroscopic and histological examination. The esophagus, heart, diaphragm, tongue, masseter, and\\u000a thigh muscles were investigated. Esophagus and thigh muscles of only 3 of the 100 examined water buffaloes (3%) were infected\\u000a with macroscopic Sarcocystis,

Ahmad Oryan; Nasrollah Ahmadi; Seyed Mostafa Modarres Mousavi

2010-01-01

9

Sarcocystis dubeyi (Huong and Uggla, 1999) infection in water buffaloes (Bubalus bubalis) from Egypt  

Technology Transfer Automated Retrieval System (TEKTRAN)

Water buffaloes (Bubalus bubalis) are intermediate hosts for 4 species of Sarcocystis, i.e., S. fusiformis and S. buffalonis with cats as definitive hosts, S. levinei with dogs as definitive hosts, and S. dubeyi with an unknown definitive host, but thought to be zoonotic. Currently, the latter speci...

10

Redescription of Sarcocystis fusiformis sarcocysts from the water buffalo (Bubalus bubalis)  

Technology Transfer Automated Retrieval System (TEKTRAN)

Five species of Sarcocystis have been reported from the water buffalo (Bubalus bubalis): Sarcocystis fusiformis and Sarcocystis buffalonis have macrocysts and cats act as definitive hosts; Sarcocystis levinei has microcysts and dogs act as definitive host; Sarcocystis dubeyi and S. sinensis have mic...

11

Shedding of Neospora caninum oocysts by dogs fed tissues from naturally infected water buffaloes ( Bubalus bubalis) from Brazil  

Microsoft Academic Search

Attempts were made to isolate Neospora caninum from naturally infected water buffaloes (Bubalus bubalis) from Brazil. Brains from six buffaloes with indirect fluorescent antibodies (>1:100) to N. caninum were used to isolate the parasite by bioassay in dogs and gerbils followed by in vitro culture. Shedding of Neospora-like oocysts was noticed in dogs fed brains from three buffaloes (isolate designation

A. A. R. Rodrigues; S. M. Gennari; D. M Aguiar; C. Sreekumar; D. E. Hill; K. B. Miska; M. C. B. Vianna; J. P. Dubey

2004-01-01

12

Abortion and foetal lesions induced by Neospora caninum in experimentally infected water buffalos (Bubalus bubalis).  

PubMed

The water buffalo (Bubalus bubalis) is an important species in several countries for its milk and meat production, as well as for transport and other agricultural activities. It is, in general, considered more resistant than cattle to different parasitic diseases, also less demanding for forage quality. It has been postulated that buffalo may be resistant to abortion caused by neosporosis, because of high serological prevalences found in buffalo herds from different localities, with no description of Neospora caninum-related abortion. Recent studies have demonstrated the potential impact of neosporosis in pregnant water buffalo cows. In this work, three pregnant buffalo cows were experimentally infected with Nc-1 strain of N. caninum, and abortion was detected 35 days post-infection. Molecular and histopathological results found in post-mortem tissues are described and discussed, confirming the susceptibility of water buffalos to abortion caused by N. caninum. PMID:25324135

Chryssafidis, Andreas L; Cantón, Germán; Chianini, Francesca; Innes, Elisabeth A; Madureira, Ed H; Soares, Rodrigo M; Gennari, Solange M

2015-01-01

13

Prevalence and molecular characterization of Sarcocystis species in water buffaloes (Bubalus bubalus) in Egypt.  

PubMed

The present study was planned to investigate the prevalence of Sarcocystis spp. among slaughtered water buffaloes (Bubalus bubalis) at Alexandria province, Egypt. Three hundred blood samples were collected from slaughtered buffaloes (5-7 years old). Two techniques were used to evaluate the seroprevalence of Sarcocystis spp., enzyme-linked immunosorbent assay (ELISA) and indirect haemagglutination assay (IHA). It was revealed that 203 (67.6 %) and 191 (63.6 %) of the tested serum samples were seropositive to Sarcocystis spp. by ELISA and IHA, respectively. The results of sensitivity and specificity of IHA relative to ELISA were 94 and 100 %, respectively. For molecular characterization of inter- and intra-species genetic polymorphism within Egyptian isolates of Sarcocystis spp. of water buffaloes, polymerase chain reaction (PCR) and polymerase chain reaction-restriction length polymorphisms (PCR-RFLPs) were performed on four macroscopic isolates. The isolates represented two different geographical regions of Egypt, Alexandria and Assuit provinces. Alexandria isolates (large and small-sized cyst of the same host) and Assuit isolates (large and small-sized cyst of the same host) were used. The 18S rDNA of the macroscopic cysts were characterized, in tandem, by four restriction endonucleases, RsaI, MboI, SspI and DraI. RsaI and MboI enzymes did not show any restriction sites for all isolates, leaving the amplified fragments without cutting. SspI showed two fragments in Alexandria and Assuit small-sized isolates cut by the enzyme at 600-700-bp fragments, while Alexandria and Assuit large-sized cysts amplicons were not digested by this enzyme. The fourth enzyme, DraI, cut the PCR product of Alexandria large-sized cysts into two fragments (420-780 bp), while Assuit large-sized amplicon was not cut. It could be concluded that there was a far distance between the two local isolates (small and large sized), but there were no differences between the large-sized isolates. PMID:24619513

Ashmawy, Karam I; Abu-Akkada, Somaia S; Ghashir, Mohamed Bn

2014-12-01

14

Neosporosis in water buffalo ( Bubalus bubalis) in southern Italy  

Microsoft Academic Search

A study was carried on 1377 water buffalo serum samples from 50 farms in southern Italy to test the presence of Neospora caninum antibodies by indirect fluorescence antibody test (IFAT). Rabbit anti-buffalo immunoglobulins conjugated to fluorescein were used in the test. Fluorescence in sera dilutions above 1:200 was considered as indicative of the presence of N. caninum antibodies. The overall

A Guarino; G Fusco; G Savini; G Di Francesco; G Cringoli

2000-01-01

15

FATAL INTESTINAL COCCIDIOSIS IN A THREE-WEEK OLD BUFFALO CALF (BUBALUS BUBALUS)  

Technology Transfer Automated Retrieval System (TEKTRAN)

The water buffalo (Bubalus bubalus) is important to the economy of several countries, especially in Asia and Brazil. Little is known regarding the impact of coccidiosis in buffaloes. Cattle and buffaloes are considered to have common species of Eimeria, but critical cross transmissions have not been...

16

Isolation of new pregnancy-associated glycoproteins from water buffalo ( Bubalus bubalis) placenta by Vicia villosa affinity chromatography  

Microsoft Academic Search

The present study describes the isolation and characterization of new pregnancy-associated glycoprotein molecules (PAG) from midpregnancy and late-pregnancy placentas in the water buffalo (Bubalus bubalis). After extraction, the homogenates are subjected to acid and ammonium sulfate precipitations followed by DEAE chromatography. Subsequently, the water buffalo PAG (wbPAG) from these solutions are enriched by Vicia villosa agarose (VVA) affinity chromatography. As

O. Barbato; N. M. Sousa; K. Klisch; E. Clerget; A. Debenedetti; V. L. Barile; A. Malfatti; J. F. Beckers

2008-01-01

17

Isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (Bubalus bubalis) in Argentina  

PubMed Central

Background Parainfluenza virus type 3 (PIV3) was isolated from dairy buffaloes (Bubalus bubalis) naturally affected with respiratory and reproductive clinical conditions. Results Examination of nasal and vaginal swabs collected from 12 diseased buffaloes led to the isolation of three paramyxovirus isolates from two animals. Antigenic, morphological and biological characteristics of these three isolates were essentially similar to those of members of the Paramyxoviridae family. Antigenic analysis by direct immunofluorescence and cross neutralization test placed these isolates together with bovine parainfluenza virus type 3 (BPIV3). Nucleotide and amino acid phylogenetic analysis of partial matrix gene sequences of the buffalo isolates and six field BPIV3 isolates from bovines in Argentina were studied. Buffalo isolates were similar to genotype B (BPIV3b) while the six BPIV3 isolates were similar to genotypes A (BPIV3a) and C (BPIV3c). Conclusions This is the first characterization of BPIV3 in water buffalo. According to the samples analyzed, in Argentina, the genotype B was found in buffalo and the genotypes A and C were found in cattle. PMID:22716217

2012-01-01

18

Effects of smallmouth buffalo, Ictiobus bubalus biomass on water transparency, nutrients, and productivity in shallow experimental ponds.  

PubMed

The smallmouth buffalo Ictiobus bubalus is a native benthivore to floodplain lakes in the Yazoo River Basin, USA. Based on evidence from other benthivorous fish studies we hypothesized high biomasses of I. bubalus contribute to poor water quality conditions. We tested this hypothesis in shallow (<1.5 m) 0.05 ha earthen ponds at three stocking biomasses over a 10-week period during the summer of 2012. The most notable results from the permutational multivariate analysis of variance suggest I. bubalus at high and moderate biomasses significantly (p < 0.05) enhanced turbidity and suspended solid levels while decreasing Secchi depth. Our results suggest that effects of I. bubalus on water clarity may have considerable ecological implications in natural habitats such as shallow floodplain lakes. PMID:24526283

Goetz, D; Kröger, R; Miranda, L E

2014-05-01

19

The Effects of Benthivorous Smallmouth Buffalo (Ictiobus bubalus) on Water Quality and Nutrient Cycling in a Shallow Floodplain Lake  

Microsoft Academic Search

Experimental mesocosm studies revealed that the presence of benthivorous smallmouth buffalo (Ictiobus bubalus) significantly enhanced turbidity, phytoplankton biomass, ammonium (NH4 ), and total nitrogen (N) and phosphorus (P) concentrations in a shallow, aerobic, hypereutrophic oxbow lake. The effects of Ictiobuson water quality are similar to the results of experiments performed on other benthivorous fish species. Prior studies have suggested that

David E. Shormann; James B. Cotner

1997-01-01

20

Binucleate trophoblast giant cells in the water buffalo (Bubalus bubalis) placenta.  

PubMed

The binucleate trophoblast giant cells (BNC) of the water buffalo, Bubalus bubalis, placenta were studied, with emphasis on the synthesis of BNC-specific proteins. Placentomal tissues of 27 water buffalos (2-10 months of pregnancy) were processed for light and electron microscopy. The frequency of BNCs was 20% of the trophoblastic cells in 2-3-month placentas and increased to 27% in the later stages. Ultrastructurally, binucleate cells displayed a prominent granular endoplasmic reticulum and Golgi apparatus, typical of cells involved with protein synthesis and exportation. The buffalo BNCs contained periodic acid-Schiff (PAS)-positive granules and reacted with antisera against bovine placental lactogen, prolactin-related protein-I, and pregnancy-associated glycoproteins. Lectin histochemistry with Dolichos biflorus agglutinin, Vicia villosa agglutinin, and Phaseolus vulgaris leucoagglutinin showed specific staining of BNCs. Different stages of BNC migration and fusion with uterine epithelial cells were observed. Trinucleate feto-maternal hybrid cells were the typical outcome of cell fusions. These cells underwent degeneration, with typical morphological features of apoptosis. The results revealed a strong homology between water buffalo and cattle BNCs concerning cell morphology, protein expression, glycosylation pattern, and characteristics of cell migration and fusion. PMID:16240388

Carvalho, A F; Klisch, K; Miglino, M A; Pereira, F T V; Bevilacqua, E

2006-01-01

21

Serum biochemical and haematological reference intervals for water buffalo Bubalus bubalis heifers.  

PubMed

Based on a review of the literature, reference intervals for water buffalo (Bubalus bubalis) serum biochemistry and haematology have not previously been published. The current study was done to establish reference intervals for water buffalo heifers. The International Federation of Clinical Chemistry stated that at least 120 values are necessary to obtain reliable estimates for reference intervals. A total number of 127 clinically healthy buffalo heifers (1-2 years old) were included in the study. Animals were examined at buffalo farms that belong to Assiut Governorate, Egypt. Three types of samples were collected: serum samples for biochemical analysis, whole blood samples for haematological analysis and faecal samples for parasitological examination. Animals that fitted the inclusion criteria were included in the study. Biochemical analysis included serum total proteins, albumin, total globulins, alpha, beta and gamma globulin levels, and aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase, creatine phosphokinase and lactate dehydrogenase activity. In addition to the above, serum creatinine, urea, total bilirubin, direct bilirubin, indirect bilirubin, sodium, potassium, chloride, magnesium, calcium, phosphorus, copper, zinc, iron, triglycerides, high density lipoprotein, low density lipoprotein, very low density lipoprotein, glucose levels and 20 haematological variables were measured. The 95.0% reference intervals were calculated by removing the upper and lower 2.5% of the interval for each serum biochemical constituent to give the 2.5 and 97.5 percentiles. Confidence intervals were calculated for each reference limit. Reference intervals from the current study were compared with established values for cows. The current study is as far as could be determined the first that establishes reference intervals for the serum biochemical and haematological parameters in water buffalo heifers. PMID:24831856

Abd Ellah, Mahmoud R; Hamed, Maha I; Ibrahim, Derar R; Rateb, Hassan Z

2014-01-01

22

Mammary diffuse fibroadenomatoid hyperplasia in water buffalo (Bubalus bubalis): three cases.  

PubMed

The current report describes 3 rare cases of mammary diffuse fibroadenomatoid hyperplasia in water buffalo (Bubalus bubalis). All of the animals were between 10 and 12 months of age. Grossly, the lesions consisted of severe diffuse swelling with homogeneous large masses in the udder. Surgical removal of the masses was curative. Microscopically, there was severe hyperplasia of the mammary epithelium and numerous well-differentiated and mildly pleomorphic acini and their associated ducts. Moderate proliferation of the fibrous connective tissue and the myoepithelial cells near the proliferating acini was also evident. The hyperplastic epithelial cells exhibited positive immunostaining for cytokeratin, estrogen receptors, and progesterone receptors. In addition, the myoepithelial cells displayed moderate positivity for alpha smooth muscle actin. Based on the clinical, morphologic, and immunohistochemical findings, a diagnosis of mammary diffuse fibroadenomatoid hyperplasia with probable hormonal influence was made. PMID:24621849

de Sant'Ana, Fabiano J F; Carvalho, Fausto C; de O Gamba, Conrado; Cassali, Geovanni D; Riet-Correa, Franklin; Schild, Ana L

2014-03-12

23

Natural Babesia bovis Infection in Water Buffaloes (Bubalus bubalis) and Crossbred Cattle under Field Conditions in Egypt: a Preliminary Study  

PubMed Central

Background There is a little or no data available on the natural Babesia bovis (B. bovis) infection in water buffaloes (Bubalus bubalis) comparing to the available one for cattle. This study was conducted to investigate the natural B. bovis infection in water buffaloes in comparison to crossbred cattle under field conditions in Egypt. Methods: A total of 35 buffaloes and cattle were clinically and laboratory investigated from March to June 2008. Twenty-nine buffaloes and cattle out of 35 were naturally infected with B. bovis and showed signs of bovine babesiosis. Three cows and three buffaloes showed no clinical signs and were free from external, internal, and blood parasites served as control group. Results: Babesia bovis-infected cattle showed typical signs of bovine babesiosis while B. bovis-infected buffaloes showed a milder form (less severe) of the clinical signs. Advanced cases of cattle showed dark brown to dark red (coffee-color) urine, hemoglobinuria and nervous manifestations while these manifestations were not detected in the infected buffaloes. Hematological changes in both species however, these changes were less significant in buffaloes than those reported in cattle. Conclusion: This paper documents the first description of natural B. bovis infection in water buffaloes which were found to be more likely to be tolerant than cattle to the natural clinical infection with B. bovis and its subsequent haematological changes. Our finding may lead to a better understanding of the disease pattern of B. bovis infection under field conditions in buffaloes.

Mahmmod, Yasser

2014-01-01

24

Immunodetection of coproantigens for the diagnosis of amphistomosis in naturally infected Indian Water Buffalo, Bubalus bubalis.  

PubMed

The infection of gastrointestinal helminths in livestock is routinely diagnosed by microscopical examination of faecal samples for the presence of ova/eggs but this approach becomes ineffective for the seasonally egg producing trematodes. Therefore, an alternative approach to detect the coproantigens of liver and rumen amphistomes, Gigantocotyle explanatum and Gastrothylax crumenifer respectively, infecting Indian water buffalo Bubalus bubalis, was undertaken using ELISA, immunodot and countercurrent immunoelectrophoresis (CCIEP). The hyperimmune polyclonal antisera were separately raised in rabbits against excretory/secretory (ES) antigens of both the flukes under study. An overall 70% buffalo faecal samples were tested positive for G. crumenifer and 75% for G. explanatum in Aligarh region. The ELISA results reflected higher infection intensity among individual buffaloes that was also observed at necropsy. Using the respective homologous hyperimmune antiserum, 55% buffaloes tested positive for G. crumenifer and 65% positive for G. explanatum in immunodot assay. Further, the faecal samples with high absorbance values in ELISA and strong immunodot reaction tested positive in CCIEP. The analysis of CCIEP result revealed two and one precipitin bands in G. crumenifer and G. explanatum respectively, indicating prominent antigenic differences in the coproantigens of these two parasites. Taken together, it is suggested that polyclonal antibodies could be conveniently used for the detection of coproantigens by ELISA and immunodot methods, particularly during the non-egg producing phase of the seasonally regulated reproductive cycle of the rumen amphistome G. crumenifer. It is concluded that the coproantigen detection is a good alternative over conventional method for the diagnosis of amphistomosis in livestock; however, further studies are required on a larger sample size of field buffaloes to augment the reproducibility of the present results. PMID:23036808

Saifullah, Mohammad K; Ahmad, Gul; Abidi, Syed M A

2013-01-16

25

First molecular characterisation of Cryptosporidium and Giardia from Bubalus bubalis (water buffalo) in Victoria, Australia.  

PubMed

We conducted a molecular epidemiological survey of Cryptosporidium and Giardia from Bubalus bubalis (water buffalo) on two extensive farms (450 km apart) in Victoria, Australia. Faecal samples (n=476) were collected from different age groups of water buffalo at two time points (six months apart) and tested using a PCR-based mutation scanning-targeted sequencing-phylogenetic approach, employing markers within the small subunit of ribosomal RNA (designated pSSU) and triose phosphate isomerase (ptpi) genes. Based on pSSU data, Cryptosporidium parvum, Cryptosporidium bovis and Cryptosporidium genotypes 1, 2 (each 99% similar genetically to Cryptosporidium ryanae) and 3 (99% similar to Cryptosporidium suis) were detected in two (0.4%), one (0.2%), 38 (8.0%), 16 (3.4%) and one (0.2%) of the 476 samples tested, respectively. Using ptpi, Giardia duodenalis assemblages A and E were detected in totals of 56 (11.8%) and six (1.3%) of these samples, respectively. Cryptosporidium was detected on both farms, whereas Giardia was detected only on farm B, and both genera were detected in 1.5% of all samples tested. The study showed that water buffaloes on these farms excreted C. parvum and/or G. duodenalis assemblage A, which are consistent with those found in humans, inferring that these particular pathogens are of zoonotic significance. Future work should focus on investigating, in a temporal and spatial manner, the prevalence and intensity of such infections in water buffaloes in various geographical regions in Australia and in other countries. PMID:23886616

Abeywardena, Harshanie; Jex, Aaron R; von Samson-Himmelstjerna, Georg; Haydon, Shane R; Stevens, Melita A; Gasser, Robin B

2013-12-01

26

doi:10.4061/2011/705358 Research Article Frequency of Toxoplasmosis in Water Buffalo (Bubalus bubalis) in Trinidad  

E-print Network

Copyright © 2011 Anil Persad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Toxoplasmosis has been reported to occur in several animals and humans causing different clinical manifestations. The study was conducted to determine the frequency of Toxoplasma gondii antibodies (IgG) in water buffalo (Bubalus bubalis) acrossfarmsin Trinidad using a latex agglutination test. Of a total of 333 water buffalo tested, 26 (7.8%) were seropositive for T. gondii antibodies. Seropositivity for toxoplasmosis was statistically significantly (P water buffalo, 12.4 % (14 of 113) compared with young water buffalo, 4.2 % (6 of 143). Seropositivity for toxoplasmosis across the seven farms ranged from 0.0 % (0 of 20) in Farm G compared with 20.0 % (10 of 50) detected in Farm B. The differences in seropositivity by management system,

Anil Persad; Roxanne Charles; Abiodun A. Adesiyun

27

Effect of Pen Size on Behavioral, Endocrine, and Immune Responses of Water Buffalo (Bubalus bubalis) Calves1,2  

Microsoft Academic Search

Female water buffalo ( Bubalus buba- lis) calves (n = 28) aged 7 to 10 d were divided into four groups of seven animals each to examine the effects of space allowance (Group A: 2.6 indoor m2 + 2.0 outdoor m2\\/calf; Group B: 2.6 indoor m2\\/calf; Group C: 1.5 indoor m2\\/calf; Group D: 1.0 indoor m2\\/calf) on behavioral, endocrine, and

Fernando Grasso; Fabio Napolitano; Giuseppe De Rosa; Teresa Quarantelli; Luigi Serpe; Aldo Bordi

28

Seroprevalence of Neospora caninum in female water buffaloes ( Bubalus bubalis) from the southeastern region of Brazil  

Microsoft Academic Search

Antibodies to Neospora caninum were assayed in sera of 222 female water buffaloes from Ribeira Valley of São Paulo State, Brazil, using an indirect fluorescent antibody test (IFAT) and Neospora agglutination test (NAT). IFAT antibodies were found in 64% of buffaloes with titers of 1:25 (42 buffaloes), 1:50 (53 buffaloes), 1:100 (31 buffaloes), 1:200 (10 buffaloes), 1:400 (3 buffaloes), or

T. U Fujii; N Kasai; S. M Nishi; J. P Dubey; S. M Gennari

2001-01-01

29

Seroprevalence of antibodies to Neospora caniuum and Toxoplasma gondii in water buffaloes ( Bubalus bubalis) from Egypt  

Microsoft Academic Search

Sera from 75 water buffaloes from Egypt were examined using a direct agglutination test incorporating mercaptoethanol for antibodies to Neospora caninum and Toxoplasma gondii. Antibodies to N. caninum were found in 51 (68%) of 75 buffaloes in titres of 1:20 (six buffaloes), 1:40 (15 buffaloes), 1:160 (one buffalo), 1:320 (one buffalo) and ?1:640 (28 buffaloes), using N. caninum formalin-preserved whole

J. P. Dubey; S. Romand; M. Hilali; O. C. H. Kwok; P. Thulliez

1998-01-01

30

Characterization of immune cell infiltration in the placentome of water buffaloes (Bubalus bubalis) infected with neospora caninum during pregnancy.  

PubMed

Neospora caninum infection in cattle stimulates host immune responses, which may be responsible for placental damage leading to abortion. Susceptibility of water buffaloes (Bubalus bubalis) to neosporosis is not well understood, although vertical transmission and fetal death have been documented. The aim of this study was to characterize the immune response in the placentome of water buffalo following experimental infection in early gestation with the Nc-1 strain of N. caninum. Placentomes were examined by immunohistochemistry using antibodies specific for T-cell subsets, natural killer cells and CD79(?cy) cells. Placental inflammation was characterized by the infiltration of CD3(+) and CD4(+) T cells and T cells expressing the ?? T-cell receptor. The distribution of these cellular subsets in buffalo placentomes was similar to that previously described in cattle infected with N. caninum in early gestation, but the lesions were milder, which may explain the lower number of abortions observed in this species after infection. PMID:24529512

Cantón, G J; Konrad, J L; Moore, D P; Caspe, S G; Palarea-Albaladejo, J; Campero, C M; Chianini, F

2014-05-01

31

Redescription of Sarcocystis fusiformis sarcocysts from the water buffalo (Bubalus bubalis).  

PubMed

SUMMARY Four valid species of Sarcocystis have been reported from the water buffalo (Bubalus bubalis): Sarcocystis fusiformis, Sarcocystis buffalonis, Sarcocystis levinei and Sarcocystis dubeyi. Here, we redescribe structure of S. fusiformis sarcocysts by scanning and transmission electron microscopy (SEM, TEM). Twenty-one macroscopic sarcocysts from oesophagus of the water buffalo in Egypt were examined by light microscopy, SEM and TEM. The sarcocyst wall was up to 9 ?m thick, depending on the section and the technique. In 5 ?m paraffin-embedded sections, the sarcocyst wall was indistinct, 2-5 ?m thick and appeared smooth. In 1 ?m plastic-embedded sections stained with toluidine blue, the sarcocyst wall was 2·5-5·2 ?m thick and had branched villar protrusions (vp)-like branches of a dead tree. By SEM, the sarcocyst wall had a mesh-like structure with irregularly shaped vp that were folded over the sarcocyst wall. On each vp there were uniform papillomatous structures that were 100 nm wide. By TEM, vp were up to 6 ?m long and contained filamentous tubular structures, most of which were parallel to the long axis of the projections; granules were absent from these tubules. By TEM, bradyzoites within the same cyst varied from 11·2 to 16·8 ?m in length. By TEM, bradyzoites had a very long (10 ?m) convoluted mitochondrion, up to 12 dense granules, but only 2 rhoptries. This redescription should help to differentiate the sarcocysts of S. fusiformis from similar sarcocysts in domestic and wild ruminants. PMID:25111676

Dubey, J P; Hilali, M; VAN Wilpe, E; Verma, S K; Calero-Bernal, R; Abdel-Wahab, A

2015-02-01

32

Identity of Sarcocystis species of the water buffalo (Bubalus bubalis) and cattle (Bos taurus) and the suppression of Sarcocystis sinensis as a nomen nudum  

Technology Transfer Automated Retrieval System (TEKTRAN)

There are uncertainties concerning the identity and host species specificity of Sarcocystis species of the water buffalo (Bubalus bubalis) and cattle (Bos taurus). Currently, in cattle three species are recognized with known endogenous stages, viz.: S. cruzi (with canine definitive host), S. hirsuta...

33

Detection of Mycobacterium avium subsp. paratuberculosis in intestinal and lymph node tissues of water buffaloes ( Bubalus bubalis) by PCR and bacterial culture  

Microsoft Academic Search

The efficacy of bacterial culture and IS900-specific polymerase chain reaction (PCR) was compared for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) from the intestinal and mesenteric lymph node tissues of water buffaloes (Bubalus bubalis) showing lesions of paratuberculosis (Johne's disease). Out of 20 (4.9%) animals showing histological lesions suggestive of paratuberculosis, 14 (70%) and 6 (30%) were positive in

P. Sivakumar; B. N. Tripathi; Nem Singh

2005-01-01

34

Isolation of new pregnancy-associated glycoproteins from water buffalo (Bubalus bubalis) placenta by Vicia villosa affinity chromatography.  

PubMed

The present study describes the isolation and characterization of new pregnancy-associated glycoprotein molecules (PAG) from midpregnancy and late-pregnancy placentas in the water buffalo (Bubalus bubalis). After extraction, the homogenates are subjected to acid and ammonium sulfate precipitations followed by DEAE chromatography. Subsequently, the water buffalo PAG (wbPAG) from these solutions are enriched by Vicia villosa agarose (VVA) affinity chromatography. As determined by western blotting with anti-PAG sera, the apparent molecular masses of the immunoreactive bands from the VVA peaks range from 59.5 to 75.8kDa and from 57.8 to 73.3kDa in the midpregnancy and late-pregnancy placentas, respectively. Amino-terminal microsequencing of the immunoreactive proteins has allowed the identification of three distinct wbPAG sequences, which have been deposited in the SwissProt database: RGSXLTIHPLRNIRDFFYVG (acc. no. P85048), RGSXLTILPLRNIID (acc. no. P85049), and RGSXLTHLPLRNI (acc. no. P85050). Their comparison to previously identified proteins has shown that two of them are new because they have not been described before. Our results confirm the suitability of VVA chromatography for the enrichment of the multiple PAG molecules expressed in buffalo placenta. PMID:18308351

Barbato, O; Sousa, N M; Klisch, K; Clerget, E; Debenedetti, A; Barile, V L; Malfatti, A; Beckers, J F

2008-12-01

35

Local immune responses of the Chinese water buffalo, Bubalus bubalis, against Schistosoma japonicum larvae: crucial insights for vaccine design.  

PubMed

Asian schistosomiasis is a zoonotic parasitic disease infecting up to a million people and threatening tens of millions more. Control of this disease is hindered by the animal reservoirs of the parasite, in particular the water buffalo (Bubalus bubalis), which is responsible for significant levels of human transmission. A transmission-blocking vaccine administered to buffaloes is a realistic option which would aid in the control of schistosomiasis. This will however require a better understanding of the immunobiology of schistosomiasis in naturally exposed buffaloes, particularly the immune response to migrating schistosome larvae, which are the likely targets of an anti-schistosome vaccine. To address this need we investigated the immune response at the major sites of larval migration, the skin and the lungs, in previously exposed and re-challenged water buffaloes. In the skin, a strong allergic-type inflammatory response occurred, characterised by leukocyte and eosinophil infiltration including the formation of granulocytic abscesses. Additionally at the local skin site, interleukin-5 transcript levels were elevated, while interleukin-10 levels decreased. In the skin-draining lymph node (LN) a predominant type-2 profile was seen in stimulated cells, while in contrast a type-1 profile was detected in the lung draining LN, and these responses occurred consecutively, reflecting the timing of parasite migration. The intense type-2 immune response at the site of cercarial penetration is significantly different to that seen in naive and permissive animal models such as mice, and suggests a possible mechanism for immunity. Preliminary data also suggest a reduced and delayed immune response occurred in buffaloes given high cercarial challenge doses compared with moderate infections, particularly in the skin. This study offers a deeper understanding into the immunobiology of schistosomiasis in a natural host, which may aid in the future design of more effective vaccines. PMID:24086786

McWilliam, Hamish E G; Piedrafita, David; Li, Yuesheng; Zheng, Mao; He, Yongkang; Yu, Xinling; McManus, Donald P; Meeusen, Els N T

2013-01-01

36

Quantitative studies on spermatogenesis in buffalo (Bubalus bubalis)  

E-print Network

Quantitative studies on spermatogenesis in buffalo (Bubalus bubalis) G. S. BILASPURI S. S. GURAYA. The qualitative behaviour of spermatogenetic cells and Sertoli cells in the buffalo (Bubalus bubalis) was studied epithelium cycle of the buffalo (Guraya and Bilaspuri, 1976a, b, c). The quanti- tative aspects

Boyer, Edmond

37

Mosaicism of 50,XX/51,XX in a Murrah buffalo Bubalus bubalis  

E-print Network

. The authors are not aware of any report of autosomal trisomy in water buffalo; however, in cattle variousNote Mosaicism of 50,XX/51,XX in a Murrah buffalo Bubalus bubalis BR Yadav S Kumar1 OS Tomer CR buffalo with irregular breeding history. The animal had mosaicism of two cell lines (51,XX in 22

Paris-Sud XI, Université de

38

Molecular Mining of Alleles in Water Buffalo Bubalus bubalis and Characterization of the TSPY1 and COL6A1 Genes  

Microsoft Academic Search

BackgroundMinisatellites are an integral part of eukaryotic genomes and show variation in the complexity of their organization. Besides their presence in non-coding regions, a small fraction of them are part of the transcriptome, possibly participating in gene regulation, expression and silencing. We studied the minisatellite (TGG)n tagged transcriptome in the water buffalo Bubalus bubalis across various tissues and the spermatozoa,

Sudeep Kumar; Ruchi Gupta; Sudhir Kumar; Sher Ali; Najib M. El-Sayed

2011-01-01

39

Improvement in the diagnosis of Brucella abortus infections in naturally infected water buffaloes ( Bubalus bubalis ) using an ELISA with a Protein-G-based indicator system  

Microsoft Academic Search

Brucellosis caused by Brucella abortus in domestic water buffaloes (Bubalus bubalis) raised under the traditional system of husbandry in northern India was diagnosed using an enzyme-linked immunosorbent assays\\u000a (ELISA) with a Protein-G-based indicator system (Protein-G ELISA). A total of 1,551 animals that are positive (N = 61), negative (N = 243), and suspected (N = 1,247) for brucellosis were examined.

Manish Kumar; Puran Chand

40

Rapid sexing of water buffalo ( Bubalus bubalis) embryos using loop-mediated isothermal amplification  

Microsoft Academic Search

Loop-mediated isothermal amplification (LAMP) is a novel DNA amplification method that amplifies a target sequence specifically under isothermal conditions. The objective of this study was to identify a Y chromosome-specific sequence in water buffalo and to establish an efficient procedure for embryo sexing by LAMP. The homologues of a Y chromosome-specific sequence, bovine repeat Y-associated.2, in swamp and river buffalo

Hiroki Hirayama; Soichi Kageyama; Yoshiyuki Takahashi; Satoru Moriyasu; Ken Sawai; Sadao Onoe; Keiko Watanabe; Shinichi Kojiya; Tsugunori Notomi; Akira Minamihashi

2006-01-01

41

Molecular cloning and expression analysis of the STAT1 gene in the water buffalo (Bubalus bubalis).  

PubMed

Signal transducer and activator of transcription 1 (STAT1) is a critical component of the transcription factor complex in the interferon (IFN) signaling pathways. Of the seven STAT isoforms, STAT1 is a key mediator of type I and type III IFN signaling, but limited information is available for the STAT genes in the water buffalo. Here, we amplified and identified the complete coding sequence (CDS) of the buffalo STAT1 gene by using reverse transcription polymerase chain reaction (RT-PCR). Sequence analysis indicated that the buffalo STAT1 gene length size was 3437 bp, containing an open reading frame (ORF) of 2244 bp that encoded 747 amino acids for the first time. The buffalo STAT1 CDS showed 99, 98, 89, 93, 86, 85, and 87 % identity with that of Bos taurus, Ovis aries, Homo sapiens, Sus scrofa, Rattus norvegicus, Mus musculus, and Capra hircus. The phylogenetic analyses revealed that the nearest relationship existed between the water buffalo and B. taurus. The STAT1 gene was ubiquitously expressed in 11 buffalo tissues by real-time PCR, whereas STAT1 was expressed at higher levels in the lymph. The STAT1 gene contained five targeted microRNA sequences compared with the B. taurus by the miRBase software that provide a fundamental for identifying the STAT1 gene function. PMID:25336386

Deng, Tingxian; Pang, Chunying; Zhu, Peng; Liao, Biyun; Zhang, Ming; Yang, Bingzhuang; Liang, Xianwei

2015-01-01

42

Molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (GM-CSF) from Indian water buffalo (Bubalus bubalis).  

PubMed

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. In this study, we report molecular cloning, sequencing and characterization of GM-CSF from Indian water buffalo, Bubalus bubalis. In addition, we performed sequence and structural analysis for buffalo GM-CSF. Buffalo GM-CSF has been compared with 17 mammalian GM-CSFs using multiple sequence alignment and phylogenetic tree. Three-dimensional model for buffalo GM-CSF and human receptor complex was built using homology modelling to study cross-reactivity between two species. Detailed analysis was performed to study GM-CSF interface and various interactions at the interface. PMID:23800159

Sugumar, Thennarasu; Pugalenthi, Ganesan; Harishankar, Murugesan; Dhinakar Raj, G

2014-02-01

43

Serum concentrations of haptoglobin and serum amyloid A in water buffaloes (Bubalus bubalis) with abomasal ulcer  

PubMed Central

To evaluate the serum concentrations of haptoglobin (Hp) and serum amyloid A (SAA) in water buffaloes with abomasal ulcers, the abomasums of 100 randomly selected water buffaloes were examined after slaughter. Type I abomasal ulcers were found in 56 out of 100 buffaloes. Serum concentrations of Hp and SAA were measured. There was no significant difference between affected and non-affected buffaloes in the serum concentrations of Hp and SAA. The serum concentrations of Hp and SAA had no significant correlation with age and the serum SAA revealed no significant correlation with the number of abomasal ulcers. A significant correlation was found between the serum Hp and the number of abomasal ulcers (r =0.29, p = 0.04). There was no significant difference in the serum concentrations of Hp and SAA between buffaloes with different ulcer locations in the abomasums. Although more work on a larger number of animals is required in this area, it seems that the measurement of the serum Hp can be used to predict the abundance of type I abomasal ulcers.

Tajik, Javad; Nazifi, Saeed; Heidari, Mahdi; Babazadeh, Marzieh

2012-01-01

44

Pharmacokinetics, urinary excretion and plasma protein binding of ofloxacin in water buffalo calves (Bubalus bubalis).  

PubMed

Pharmacokinetics and urinary excretion of an intravenous dose of 5 mg.kg-1 ofloxacin were investigated in water buffalo calves. Plasma concentrations of ofloxacin were determined by high-performance liquid chromatography. Ofloxacin was rapidly distributed from the central to the peripheral compartment as evidenced by a short distribution half-life (0.09 h ± 0.003 h) and high K12 (4.7 h(-1) ± 0.1 h(-1)), and was detected in plasma for 8 h. The large volume of distribution (2.48 L.kg(-1) ± 0.18 L.kg(-1)) obtained in this study indicated high distribution of ofloxacin in water buffalo calves. The elimination half-life, the area under the plasma drug concentration-time curve and total body clearance were 2.11 h ± 0.13 h, 6.20 µg.mL(-1) ± 0.23 µg.mL(-1).h and 0.81 mL.kg(-1).h(-1) ± 0.03 mL.kg(-1).h(-1), respectively. About 18.7% of administered drug was bound to plasma proteins and approximately 32.5% of the administered dose was recovered in urine within 48 h. The results of the study indicated a favourable pharmacokinetic profile of ofloxacin in water buffalo calves, which suggests that ofloxacin may be effective against urinary pathogens in this species. PMID:23718538

Ola, Ajay K; Sandhu, Harpal S; Dumka, Vinod K; Ranjan, Bibhuti

2013-01-01

45

Comparison of pharmacokinetics of marbofloxacin after subcutaneous administration of various multiple-dose regimens to water buffalo calves (Bubalus bubalis).  

PubMed

Objective-To determine pharmacokinetics of marbofloxacin in water buffalo calves (Bubalus bubalis) after multiple SC administrations and to assess differences in regimen efficacy. Animals-18 healthy buffalo calves. Procedures-Calves (n = 6 calves/group) were assigned to receive marbofloxacin SC in the neck at 1 of 3 dosages (2 mg/kg, q 24 h for 6 days [regimen 1]; 4 mg/kg, q 48 h for 6 days [regimen 2]; and 4 mg/kg, q 24 h for 3 days [regimen 3]). Serum marbofloxacin concentrations were analyzed. Efficacy predictors were estimated on the basis of minimum inhibitory concentration and mutant prevention concentration reported for Pasteurella multocida and Mannheimia haemolytica. Results-Mean ± SD area under the concentration-time curve was 5.92 ± 0.40 ?g•h/mL for regimen 1, which differed significantly from that for regimens 2 (14.26 ± 0.92 ?g•h/mL) and 3 (14.17 ± 0.51 ?g•h/mL). Mean residence time and mean elimination half-life for regimen 2 (9.93 ± 0.20 hours and 8.77 ± 0.71 hours) both differed significantly from those for regimens 1 (721 ± 0.11 hours and 5.71 ± 0.38 hours) and 3 (759 ± 0.13 hours and 737 ± 1.19 hours). Values obtained from indices for P multocida and M haemolytica had an excessively wide range because of the various degrees of antimicrobial susceptibility (low, medium, and high) of the strains. Conclusions and Clinical Relevance-Regimen 3 had the most favorable indices, and it would be conducive for owner compliance and require less handling of animals. PMID:25419804

Baroni, Eduardo E; Rubio, Sonia; De Lucas, José J; Andrés, María D San; Andrés, Manuel I San

2014-12-01

46

Identity of Sarcocystis species of the water buffalo (Bubalus bubalis) and cattle (Bos taurus) and the suppression of Sarcocystis sinensis as a nomen nudum.  

PubMed

There are uncertainties concerning the identity and host species specificity of Sarcocystis species of the water buffalo (Bubalus bubalis) and cattle (Bos taurus). Currently, in cattle three species are recognized with known endogenous stages, viz.: S. cruzi (with canine definitive host), S. hirsuta (feline definitive host), and S. hominis (primate definitive host). Recently, a fourth Sarcocystis species with an unknown life cycle has been reported from cattle. In the water buffalo, four species of Sarcocystis have been described: S. fusiformis (feline definitive host), S. buffalonis (feline definitive host), S. levinei (canine definitive host), and S. dubeyi (definitive host unknown but not cat or dog). Besides, there are studies of Sarcocystis infections in buffalo and cattle from China with results that are difficult to interpret and validate. For example, some of the studies report transmission of Sarcocystis species between cattle and buffalo, but steps to preclude exogenous exposures were not reported. A species of the water buffalo, 'S. sinensis', was proposed at a Chinese national conference in 1990, and published as an abstract without figures and with no archived type specimens for verification. The International Code of Zoological Nomenclature Articles 9 and 10 state that "abstracts of articles, papers, posters, text of lectures, and similar material when issued primarily to participants at meetings, symposia, colloquia or congress does not constitute published work"; therefore, S. sinensis is a nomen nudum. PMID:25034134

Dubey, J P; Fayer, R; Rosenthal, B M; Calero-Bernal, R; Uggla, A

2014-09-15

47

Molecular characterization of oxytocin receptor gene in water buffalo (Bubalus bubalis).  

PubMed

Buffaloes are known for their productivity as compared to average yielding cows due to higher fat percentage, better feed conversion ability and disease resistance. On the other hand, the reproductive performances of buffaloes are often considered as poor owing to late sexual maturity, weak/silent oestrus, repeat breeder and prolonged intercalving interval. The study of cascade of events during oestrus and oestrous cycle can be useful for the improvement of reproductive efficiency of buffaloes. More precisely, the hormonal changes initiated at the molecular level within the animal determine the reproductive nature of the species. Nucleotide/protein sequence analysis serves as a vital tool in analysing the binding of the hormones for their effect or functions. In this study, we have reported cloning and characterization of the complete coding (cDNA) sequence of oxytocin receptor gene (OXTR) in buffaloes. Buffalo OXTR gene contains an uninterrupted ORF of 1176 nucleotides corresponding to an inferred polypeptide length of 391 amino acids (aa). The molecular weight of the deduced aa sequence was found to be 43 kDa with an isoelectric point of 9.253 and 16.328 charge at pH 7.0. The deduced protein sequence consists of 38 strongly basic (+) (K,R), 22 strongly acidic (-) (D,E), 186 hydrophobic (A, I, L, F, W, V) and 95 Polar (N, C, Q, S, T, Y) aa. Results indicated that aspartate (D) at aa position 85 and D, R and C at aa positions 136, 137 and 138, respectively, are conserved in buffaloes. The buffalo OXTR gene shared a per cent similarity ranging from 84.7 to 98.1 and 88.5 to 97.7 at nucleotide and deduced aa sequence levels, respectively, with that of other species. Phylogram constructed on the basis of either nucleotide or deduced aa sequences of buffalo OXTR gene showed that buffalo, cattle and sheep have diverged from human and swine and formed a separate clad. The buffalo sequence has shown maximum similarity and closeness with cattle followed by sheep both at nucleotide and at aa level. PMID:25132183

Arunmozhi, N; Singh, S K; Sarath, T; Agarwal, S K; Doiphode, A; Shankar, U

2014-10-01

48

Seroepidemiology of Neospora caninum and Toxoplasma gondii in cattle and water buffaloes ( Bubalus bubalis) in the People's Republic of China  

Microsoft Academic Search

A seroepidemiological survey of Neospora caninum and Toxoplasma gondii in cattle and water buffaloes was carried out in the People's Republic of China. Serum samples were obtained from dairy (n=262, 9 herds in 9 provinces) and beef cattle (n=10, 1 herd) and water buffaloes (n=40) in China. All sera were tested for antibodies to N. caninum and T. gondii by

Jinhai Yu; Zhaofei Xia; Qun Liu; Jing Liu; Jun Ding; Wei Zhang

2007-01-01

49

Identification of pregnancy-associated glycoproteins by peptide mass fingerprinting in water buffalo (Bubalus bubalis).  

PubMed

Ruminant placentas synthesize pregnancy-associated glycoproteins (PAGs) during pregnancy, which serve as biomarkers of pregnancy. The present study was conducted to verify, whether PAGs are expressed in buffalo placenta by using lectin-based affinity chromatography and peptide mass finger printing (PMF). Fetal cotyledonary tissues were collected from gravid uteri procured from slaughtered house. Proteins were extracted and subjected to wheat germ agglutinin (WGA) lectin affinity chromatography to isolate the PAGs. The isolated glycoproteins were separated by one-dimensional SDS-PAGE. PMF results of the 75 kDa protein revealed presence of two PAGs (PAG-7 and -11). The PAG-7 consisted of about 170 mass signals, of which 16 were assigned to corresponding/translated cDNA sequences of buffalo PAG-7, leading to sequence coverage of 40%. PMF result of PAG-11 showed 170 mass signals, of which 15 were assigned to buffalo PAG-11, leading to sequence coverage of 34%. In conclusion, the glycoprotein isolated from placental extract corresponding to 75 kDa band on SDS PAGE gel was a mixture of PAG-7 and -11, which may help in development of suitable diagnostics for pregnancy in buffalo. PMID:25296505

Kumar, Pradeep; Saxena, Abhishake; Singh, S K; Sharma, R K; Singh, I; Agarwal, S K

2014-08-01

50

The Sertoli cell of the water buffalo ( Bubalus bubalis ) during the spermatogenic cycle  

Microsoft Academic Search

Ultrastructural features and morphometric evaluations of buffalo Sertoli cells are reported for the six phases of the spermatogenic cycle. The phases of the tubular seminiferous epithelium are identified according to characteristic cellular associations with completed spermiation as demarcation between two cycles. Average tubular diameter (245 µm) and epithelial height (61 µm) do not vary significantly during the cycle. The relative

Harcharan Singh Pawar; Karl-Heinz Wrobel

1991-01-01

51

Pheno- and genotyping of Brucella abortus biovar 5 isolated from a water buffalo (Bubalus bubalis) fetus: First case reported in the Americas.  

PubMed

An isolate of Brucella spp. from an aborted water buffalo (Bubalus bubalis) fetus was characterized based on its pheno- and genotype. The phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with Brucella A and M monospecific antisera. The genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence IS711 in the Brucella chromosome via B. abortus-B. melitensis-B. ovis-B. suis (AMOS)-PCR. Unexpectedly, the isolate showed a phenotype different from B. abortus bv 1, the most prevalent strain in cattle in Argentina, and from vaccine strain 19, currently used in bovines and water buffaloes. Genotyping supported the phenotypic results, as the analysis of the omp2ab gene sequence showed an identical pattern to either B. abortus bv 5 or B. melitensis. Finally, the AMOS PCR generated a 1700-bp fragment from the isolate, different than those amplified from B. abortus bv 1 (498bp) and B. melitensis (731bp), confirming the presence of B. abortus bv 5. The OIE/FAO Reference Laboratory for Brucellosis confirmed this typing. This is the first report of B. abortus bv 5 from a water buffalo in the Americas. PMID:25113673

Martínez, Diana; Thompson, Carolina; Draghi, Graciela; Canavesio, Vilma; Jacobo, Roberto; Zimmer, Patricia; Elena, Sebastián; Nicola, Ana M; de Echaide, Susana Torioni

2014-09-17

52

Seroprevalence and risk factors associated with exposure of water buffalo (Bubalus bubalis) to Neospora caninum in northeast Thailand.  

PubMed

Water buffalo are important draft animals for agriculture in resource-restricted areas worldwide. Water buffalo were shown to be experimentally susceptible to infection with Neospora caninum, potentially affected by neosporosis, and naturally exposed to the parasite in Asia. Although enzootic to Thailand, the distribution of N. caninum among Thai water buffalo is unclear. The objectives of this study were to determine the seroprevalence of N. caninum among water buffalo of northeast Thailand and to identify risk factors associated with their exposure to N. caninum. Sera from 628 water buffalo from 288 farms were tested with an indirect fluorescent antibody test (IFAT). A total of 57 samples from 48 herds contained antibodies to N. caninum, indicating overall seroprevalence of 9.1% and 16.7% among individual animals and herds, respectively. The overall seroprevalence was highest in provinces located in the Khorat Basin in the southern part of the region tested. Host age was also associated with seroprevalence, with the greatest seroprevalence (16.1%) among buffalo over 10 years of age, followed by 5-10 years of age (13.4%), 3-5 years (9.2%), and less than 3 years (1.2%). These results collectively suggested that horizontal transmission from canine definitive hosts was an important route of water buffalo exposure to N. caninum. These results also verified the importance of risk factor analysis for effective bovine neosporosis control strategies at the local level. PMID:25499824

Kengradomkij, Chanya; Inpankaew, Tawin; Kamyingkird, Ketsarin; Wongpanit, Kannika; Wongnakphet, Sirichai; Mitchell, Thomas J; Xuan, Xuenan; Igarashi, Ikuo; Jittapalapong, Sathaporn; Stich, Roger W

2015-01-15

53

Evidence for bovine besnoitiosis in Egypt-first serosurvey of Besnoitia besnoiti in cattle and water buffalo (Bubalus bubalis) in Egypt.  

PubMed

The present study evaluated the presence of specific antibodies against Besnoitia besnoiti in cattle and water buffalo (Bubalus bubalis) in Egypt. Sera from cattle (n?=?216) and water buffaloes (n?=?133) collected from five different provinces of Egypt (Behera, Alexandria, Assuit, Gharbia, and Matrouh) were analyzed. Testing for B. besnoiti antibodies by PrioCHECK® Besnoitia Ab 2.0 ELISA initially identified 13.75 % (48 out of 349) of individual sera as positive at the manufacturer's suggested cutoff threshold, 15 percent positivity (PP). Statistically significant associations between B. besnoiti prevalence, species, sex, age, and geographical distribution were observed. Seropositive animals were distributed in all of the provinces from which animals were sampled except Gharbia province. Assuit province showed the highest percentage of infection (30.76 %) followed by Matrouh, Alexandria, and Behera provinces (25, 16.29, and 9.6 %, respectively). The highest infection rate of B. besnoiti was significantly higher in cattle (17.13 %) than in water buffaloes (9.02 %). Positive cases were observed in all age categories. While the highest infection rate (17.13 %) was recorded in the age group 5-10 years followed by the age group 1-5 years (15.38 %), and only one positive case (1.58 %) was recorded in the age group less than 1 year. The highest infection rate of B. besnoiti infection was recorded in the female animals (14.95 %) followed by the male animals (8.33). This is the first report on the detection of B. besnoiti in cattle and water buffaloes in Egypt. PMID:24375275

Ashmawy, Karam Imam; Abu-Akkada, Somaia Saif

2014-03-01

54

Effect of supplemental light on growth, prolactin, progesterone and luteinizing hormone in water buffalo ( Bubalus bubalis)  

NASA Astrophysics Data System (ADS)

Fifty non-pregnant Surti buffalo heifers aged between 17 and 42 months ( n=24, <24 months; n=26, >24 months) were randomly assigned to groups subject to either natural daylight +4h supplemental light ( n=25) or natural day light ( n=25), to study changes in growth, serum prolactin (Prl), progesterone (P4) and luteinizing hormone (LH) to supplemental lighting. Ambient temperatures (T) and relative humidity (RH) generally were >27° C and <70% during the day-time, respectively. Light-supplemented heifers had 16.2 kg net body weight (BW) gain at 9 weeks compared to 20.8 kg for controls, but higher mean Prl after 6.5 weeks ( P<0.01), and higher P4 (0.41 vs 0.19 ng/ml; P<0.06) than control heifers. Older heifers had 39.7% greater BW ( P<0.01), but a net 4.3% BW gain compared to a 10.1% gain for younger heifers at 10 weeks. Older, light-supplemented heifers had higher mean P4 (0.63 vs 0.19 ng/ml; P<0.07) than the other groups. These weight and hormonal changes suggest that 4 h supplemental light can alter growth and endocrine function in buffaloes under similar planes of nutrition. While light supplementation did not have a positive effect on body wieght during the 10 week study, body weight and endocrine changes due to supplemental light may be important factors for initiation of reproductive cyclicity.

Perera, K. S.; Gwazdauskas, F. C.; Akers, R. M.; McGilliard, M. L.

1989-06-01

55

The in vitro effect of leptin on semen quality of water buffalo (Bubalus bubalis) bulls  

PubMed Central

The purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and DNA damage). Semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ?C with tris-based extender containing 0 (control), 10, 20, 50, 100, and 200 ng mL-1 leptin. The diluted semen was kept 4 hr in refrigerator to reach to the equilibration time and then packed in 0.5 mL French straws and frozen in liquid nitrogen. Our results showed that, in the fresh semen, no significant difference was observed in all sperm quality parameters evaluated among all of the examined leptin concentrations. Addition of 10 ng mL-1 leptin into semen extender significantly preserved sperm motility, all of the motility parameters, and viability in equilibrated semen compared to that of control group. However, in vitro addition of 200 ng mL-1 leptin, significantly decreased theses parameters. In the frozen thawed semen, all leptin concentrations decreased sperm motility and viability, but significant decrease was observed in concentrations of 100 and 200 ng mL-1. Adding leptin to semen extender did not have any significant influence on sperm DNA damage and sperm membrane integrity in all examined groups. These findings suggest that in vitro addition of 10 ng mL-1 leptin could preserve sperm motility and viability in cooled semen of buffaloes.

Khaki, Amir; Batavani, Rooz Ali; Najafi, Gholamreza

2013-01-01

56

Molecular cloning, sequence characterization, and gene expression profiling of a novel water buffalo (Bubalus bubalis) gene, AGPAT6.  

PubMed

Several 1-acylglycerol-3-phosphate-O-acyltransferases (AGPATs) can acylate lysophosphatidic acid to produce phosphatidic acid. Of the eight AGPAT isoforms, AGPAT6 is a crucial enzyme for glycerolipids and triacylglycerol biosynthesis in some mammalian tissues. We amplified and identified the complete coding sequence (CDS) of the water buffalo AGPAT6 gene by using the reverse transcription-polymerase chain reaction, based on the conversed sequence information of the cattle or expressed sequence tags of other Bovidae species. This novel gene was deposited in the NCBI database (accession No. JX518941). Sequence analysis revealed that the CDS of this AGPAT6 encodes a 456-amino acid enzyme (molecular mass = 52 kDa; pI = 9.34). Water buffalo AGPAT6 contains three hydrophobic transmembrane regions and a signal 37-amino acid peptide, localized in the cytoplasm. The deduced amino acid sequences share 99, 98, 98, 97, 98, 98, 97 and 95% identity with their homologous sequences from cattle, horse, human, mouse, orangutan, pig, rat, and chicken, respectively. The phylogenetic tree analysis based on the AGPAT6 CDS showed that water buffalo has a closer genetic relationship with cattle than with other species. Tissue expression profile analysis shows that this gene is highly expressed in the mammary gland, moderately expressed in the heart, muscle, liver, and brain; weakly expressed in the pituitary gland, spleen, and lung; and almost silently expressed in the small intestine, skin, kidney, and adipose tissues. Four predicted microRNA target sites are found in the water buffalo AGPAT6 CDS. These results will establish a foundation for further insights into this novel water buffalo gene. PMID:24114207

Song, S; Huo, J L; Li, D L; Yuan, Y Y; Yuan, F; Miao, Y W

2013-01-01

57

A possible case of caprine-associated malignant catarrhal fever in a domestic water buffalo (Bubalus bubalis) in Switzerland  

PubMed Central

Background Malignant catarrhal fever (MCF) is a fatal herpesvirus infection, affecting various wild and domestic ruminants all over the world. Water buffaloes were reported to be particularly susceptible for the ovine herpesvirus-2 (OvHV-2) causing the sheep-associated form of MCF (SA-MCF). This report describes the first case of possibly caprine-associated malignant catarrhal fever symptoms in a domestic water buffalo in Switzerland. Case presentation The buffalo cow presented with persistent fever, dyspnoea, nasal bleeding and haematuria. Despite symptomatic therapy, the buffalo died and was submitted to post mortem examination. Major findings were an abomasal ulceration, a mild haemorrhagic cystitis and multifocal haemorrhages on the epicardium and on serosal and mucosal surfaces. Eyes and oral cavity were not affected. Histopathology revealed a mild to moderate lymphohistiocytic vasculitis limited to the brain and the urinary bladder. Although these findings are typical for MCF, OvHV-2 DNA was not detected in peripheral blood lymphocytes or in paraffin-embedded brain, using an OvHV-2 specific real time PCR. With the aid of a panherpesvirus PCR, a caprine herpesvirus-2 (CpHV-2) sequence could be amplified from both samples. Conclusions To our knowledge, this is the first report of malignant catarrhal fever in the subfamily Bovinae, where the presence of CpHV-2 could be demonstrated. The etiological context has yet to be evaluated. PMID:22132808

2011-01-01

58

Impact of Livestock Hygiene Education Programs on Mastitis in Smallholder Water Buffalo (Bubalus bubalis) in Chitwan, Nepal  

PubMed Central

A project implemented from 2003–2005 trained women in Chitwan District, Nepal, in hygienic dairy production using a process of social mobilization. The aim of this research was to assess if the prevalence of mastitis in water buffalo in the households of women who were trained was lower one year after training than in untrained households, if the training influenced knowledge and practices for the prevention or control of mastitis, and if these practices and knowledge were associated with a lower prevalence of mastitis. A total of 202 households from Eastern and Western Chitwan District were included in the study. Of these, 60 households had participated in the project and 142 had not. Milk samples were collected from 129 households (33 project households and 96 non-project households). Clinical mastitis was determined using visual inspection of udders and detection of macroscopic clots and flakes in milk. The California Mastitis Test was used to diagnose sub-clinical mastitis from milk samples, and the IDEXX SNAP test to identify the presence of tetracycline residues. The prevalence of mastitis in trained households (39.4%) was 43.78% of that in untrained households (60.4%), lower but not significantly so (p = 0.08, 95% CI 0.17–1.12). Thirteen indicators of knowledge or practice for the control or prevention of mastitis were more likely to occur in trained households, four significantly so (not consuming milk from sick buffalo (p=0.001), using soap to wash hands before milking (p=0.001), discarding milk after antibiotic usage (p=0.01), and choosing appropriate flooring for their livestock (p=0.03)). Trained households that discarded milk from sick buffalo were 2.96 times more likely to have at least one animal with mastitis in the household (p=0.03, 95% CI 1.15–7.65). Trained households that knew to wash buffalos teats after milking were less likely (OR 0.25) to have mastitis in their herd ((p=0.02, 95% CI 0.08–0.80). Of the 138 buffalos tested, only one tested positive for tetracycline residues. PMID:20655119

Ng, Linda; Jost, Christine; Robyn, Misha; Dhakal, I.P.; Bett, Bernard; Dhakal, Pramod; Khadkha, Rupak

2010-01-01

59

Protective Effect of the Nramp1 BB Genotype against Brucella abortus in the Water Buffalo (Bubalus bubalis)?  

PubMed Central

We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3? untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell. PMID:17145946

Capparelli, Rosanna; Alfano, Flora; Amoroso, Maria Grazia; Borriello, Giorgia; Fenizia, Domenico; Bianco, Antonio; Roperto, Sante; Roperto, Franco; Iannelli, Domenico

2007-01-01

60

On the leakage of acrosomal hyaluronidase from the spermatozoa of the buffalo (Bubalus bubalis)  

E-print Network

On the leakage of acrosomal hyaluronidase from the spermatozoa of the buffalo (Bubalus bubalis) N. The sonication method produced a quantitatively higher release of buffalo sperm hyaluronidase than the freeze. Unlike cattle semen, the seminal plasma enzyme level in buffalo semen stored at 37 °C showed a sharp rise

Boyer, Edmond

61

Morphological, biochemical and physiological studies on the preservation of buffalo (Bubalus bubalis) spermatozoa  

E-print Network

Morphological, biochemical and physiological studies on the preservation of buffalo (Bubalus of the effects of chloroquine-diphosphate (a membrane stabilizer) on the preservation of buffalo spermatozoa). Introduction. Buffalo plays an important role in the dairy economy of Asia. Although in India itself

Paris-Sud XI, Université de

62

Detailed description of RBA-banded chromosomes of river buffalo (Bubalus bubalis L.)  

E-print Network

Detailed description of RBA-banded chromosomes of river buffalo (Bubalus bubalis L.) D. DI Portici, Naples, Italy Summary The RBA banding pattern of river buffalo chromosomes and its diagrammatic comparative studies among the members of the family. Key words : River buffalo, RBA banding, chromosomes

Paris-Sud XI, Université de

63

Mitochondrial genome of Babesia orientalis, apicomplexan parasite of water buffalo (Bubalus babalis, Linnaeus, 1758) endemic in China  

PubMed Central

Background Apicomplexan parasites of the genus Babesia, Theileria and Plasmodium are very closely related organisms. Interestingly, their mitochondrial (mt) genomes are highly divergent. Among Babesia, Babesia orientalis is a new species recently identified and specifically epidemic to the southern part of China, causing severe disease to water buffalo. However, no information on the mt genome of B. orientalis was available. Methods Four pairs of primers were designed based on the full genome sequence of B. orientalis (unpublished data) and by aligning reported mt genomes of B. bovis, B. bigemina, and T. parva. The entire mt genome was amplified by four sets of PCR. The obtained mt genome was annotated by aligning with published apicomplexan mt genomes and Artemis software v11. Phylogenetic analysis was performed by using cox1 and cob amino acid sequences. Results The complete mt genome of B. orientalis (Wuhan strain) was sequenced and characterized. The entire mt genome is 5996 bp in length with a linear form, containing three protein-coding genes including cytochrome c oxidase I (cox1), cytochrome b (cob) and cytochrome c oxidase III (cox3) and six rRNA large subunit gene fragments. The gene arrangement in B. orientalis mt genome is similar to those of B. bovis, B. gibsoni and Theileria parva, but different from those of T. orientalis, T. equi and Plasmodium falciparum. Comparative analysis indicated that cox1 and cob genes were more conserved than cox3. Phylogenetic analysis based on amino acid sequences of cox1, cob and cox1 + cob, respectively, revealed that B. orientalis fell into Babesia clade with the closest relationship to B. bovis. Conclusions The availability of the entire mt genome sequences of B. orientalis provides valuable information for future phylogenetic, population genetics and molecular epidemiological studies of apicomplexan parasites. PMID:24580772

2014-01-01

64

Effects of in vitro copper sulphate supplementation on the ejaculated sperm characteristics in water buffaloes (Bubalus bubalis)  

PubMed Central

This study was carried out to investigate effects of copper sulphate (CuSO4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and DNA damage, after semen dilution and cryopreservation. Semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. A total number of 25 samples were used in each examination. Sperm progressive motility and viability were measured at 0 (T0), 60 (T1) and 120 (T2) min after diluting semen in tris-citric acid extender containing 0 (control), 0.004, 0.008, 0.016, 0.032 and 0.064 mg L-1 CuSO4. Later, semen was diluted in a tris-citric acid-egg yolk-glycerol extender containing the same amounts of CuSO4, cooled to 4 ?C and kept refrigerated for 4 hr to equilibrate, sperm progressive motility, viability, membrane integrity and DNA damage were estimated. Then, semen was packed in 0.5 mL French straws and frozen in liquid nitrogen. Later, the frozen semen was thawed in 37 ?C water bath for 30 sec, and the same parameters as well as total antioxidant capacity (TAC) of the frozen-thawed semen were estimated. The results showed that copper additive at the rate of 0.032 mg L-1 gives a better protection of sperms through the process of dilution, equilibration and freeze-thawing than that in control and other Cu concentrations, while 0.064 mg L-1 CuSO4 had deleterious effect on the sperm.

Tabassomi, Mehdi; Alavi-Shoushtari, Sayed Mortaza

2013-01-01

65

Analysis of SLC11A1 gene expression in healthy water buffalo (Bubalus bubalis) blood cells using qPCR.  

PubMed

SLC11A1 (solute carrier family 11 member 1 protein) gene influences the initial phase of bacterial cellular infections through macrophage activation. Recent literature on buffalo has attempted to associate the genotype of the polymorphic microsatellite located in the 3'untranslated region (3'UTR) of the gene, with either susceptibility to brucellosis or with improved macrophage function. Carriers of the (GT)16 allele have been reported to be resistant to brucellosis. In this study we analyzed the steady-state level of SLC11A1 expression in a serologically negative herd of 26 animals differing by the number of (GT)n microsatellite repeats by using a reverse transcriptase quantitative real-time polymerase chain reaction approach. We evaluated five different reference genes, which had not been reported previously, for use in gene expression experiments in buffalo blood. However, we did not find any significant difference between buffalo carriers of the different microsatellite alleles, with respect to SLC11A1 expression in whole blood or in blood fractions [peripheral blood mononuclear cells (PBMC) and polymorphonuclear leukocytes/granulocytes (PMN/G)]. Conversely, there was a difference between the blood fractions in their SLC11A1 expression levels, with the PMN/G fraction having a higher expression level than the PBMC fraction (P < 0.015). PMID:24391044

Crisà, A; De Matteis, G; Scatà, M C; Moioli, B

2013-01-01

66

Cystic echinococcosis in water buffaloes: Epidemiological survey and molecular evidence of ovine (G1) and buffalo (G3) strains  

Microsoft Academic Search

A survey of cystic echinococcosis (CE) in the water buffalo (Bubalus bubalis) of the Italian Mediterranean breed was carried out in Campania, a region of southern Italy. In addition, a molecular study was performed on 48 hydatid cysts coming from 48 water buffaloes in order to determine the Echinococcus granulosus strain(s) present in this host. Out of a total of

F. Capuano; L. Rinaldi; M. P. Maurelli; A. G. Perugini; V. Veneziano; G. Garippa; C. Genchi; V. Musella; G. Cringoli

2006-01-01

67

Production of wild buffalo (Bubalus arnee) embryos by interspecies somatic cell nuclear transfer using domestic buffalo (Bubalus bubalis) oocytes.  

PubMed

The objective of this study was to explore the possibility of producing wild buffalo embryos by interspecies somatic cell nuclear transfer (iSCNT) through handmade cloning using wild buffalo somatic cells and domestic buffalo (Bubalus bubalis) oocytes. Somatic cells derived from the ear skin of wild buffalo were found to express vimentin but not keratin and cytokeratin-18, indicating that they were of fibroblast origin. The population doubling time of skin fibroblasts from wild buffalo was significantly (p < 0.05) higher, and the cell proliferation rate was significantly (p < 0.05) lower compared with that of skin fibroblasts from domestic buffalo. Neither the cleavage (92.6 ± 2.0% vs 92.8 ± 2.0%) nor the blastocyst rate (42.4 ± 2.4% vs 38.7 ± 2.8%) was significantly different between the intraspecies cloned embryos produced using skin fibroblasts from domestic buffalo and interspecies cloned embryos produced using skin fibroblasts from wild buffalo. However, the total cell number (TCN) was significantly (p < 0.05) lower (192.0 ± 25.6 vs 345.7 ± 42.2), and the apoptotic index was significantly (p < 0.05) higher (15.1 ± 3.1 vs 8.0 ± 1.4) for interspecies than that for intraspecies cloned embryos. Following vitrification in open-pulled straws (OPS) and warming, although the cryosurvival rate of both types of cloned embryos, as indicated by their re-expansion rate, was not significantly different (34.8 ± 1.5% vs 47.8 ± 7.8), the apoptotic index was significantly (p < 0.05) higher for vitrified-warmed interspecies than that for corresponding intraspecies cloned embryos (48.9 ± 7.2 vs 23.9 ± 2.8). The global level of H3K18ac was significantly (p < 0.05) lower in interspecies cloned embryos than that in intraspecies cloned embryos. The expression level of HDAC1, DNMT3a and CASPASE3 was significantly (p < 0.05) higher, that of P53 was significantly (p < 0.05) lower in interspecies than in intraspecies embryos, whereas that of DNMT1 was similar between the two types of embryos. In conclusion, these results demonstrate that wild buffalo embryos can be produced by iSCNT. PMID:24494649

Priya, D; Selokar, N L; Raja, A K; Saini, M; Sahare, A A; Nala, N; Palta, P; Chauhan, M S; Manik, R S; Singla, S K

2014-04-01

68

Nucleotide diversity of mitochondrial DNAs between the swamp and the river types of domestic water buffaloes, Bubalus bubalis , based on restriction endonuclease cleavage patterns  

Microsoft Academic Search

Cleavage patterns of mitochondrial DNAs (mtDNAs) by 15 restriction endonucleases were analyzed for 10 swamp and 13 river types of domestic water buffaloes. Digestions with nine enzymes exhibited polymorphisms giving two or three kinds of cleavage patterns. Five mtDNA types were identified, three types in the swamp buffaloes of the Philippines, Vietnam, and Indonesia (S-types) and two types in the

Kazuaki Tanaka; Takahiro Yamagata; Joseph S. Masangkay; Muhammad O. Faruque; Dang Vu-Binh; Salundik; Sri Supraptini Mansjoer; Yoshi Kawamoto; Takao Namikawa

1995-01-01

69

Effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (Bubalus bubalis)  

PubMed Central

The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ?C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 µg mL-1 sodium selenite and the sperm motility and viability were evaluated at 0 (T0) (immediately after dilution), 60 (T1) and 120 (T2) min after diluting semen. In the second step, semen samples were diluted with tris-egg yolk-glycerol extender containing the same amounts of sodium selenite, cooled to 4 ?C, equilibrated and semen parameters (motility, viability, membrane integrity and DNA damage) were estimated. Then, the semen was packed in 0.5 mL French straws and frozen in liquid nitrogen. Later, the semen was thawed and analyzed for the same parameters, as well as total antioxidant capacity. Results showed that addition of 1 and 2 µgmL-1 selenium to the semen extender significantly increased the sperm motility of fresh and equilibrated semen compared to the control without affecting other parameters. However, in frozen-thawed semen, extenders containing 1 and 2 µg mL-1 selenium significantly improved sperm motility, viability, membrane integrity and semen total antioxidant capacity and also resulted in lower DNA damaged sperms. In this study selenium supplementation of semen extender of 4 and 8 µg mL-1 had deleterious effects on sperm parameters as early as the samples were prepared for freezing.

Dorostkar, Kamran; Alavi-Shoushtari, Sayed Mortaza; Mokarizadeh, Aram

2012-01-01

70

Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females  

PubMed Central

Background Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of several pregnancy-associated glycoproteins from water buffalo placenta (wbPAGs). A specific radioimmunoassay (RIA) was developed for early pregnancy diagnosis in buffalo species. Results Amino-terminal microsequencing of immunoreactive placental proteins allowed the identification of eleven wbPAGs sequences [Swiss-Prot accession numbers: P86369 to P86379]. Three polyclonal antisera (AS#858, AS#859 and AS#860) were raised in rabbits against distinct wbPAG fractions. A new RIA (RIA-860) was developed and used to distinguish between pregnant (n?=?33) and non-pregnant (n?=?26) water buffalo females. Conclusions Our results confirmed the multiplicity of PAG expression in buffalo placenta. In addition, the RIA-860 system was shown to be sensitive, linear, reproducible, accurate and specific in measuring PAG concentrations in buffalo plasma samples from Day 37 of gestation onwards. PMID:23634647

2013-01-01

71

Synchronization of follicular wave emergence following ultrasound-guided transvaginal follicle ablation or estradiol-17? administration in water buffalo (Bubalus bubalis).  

PubMed

The aim of this study was to assess the synchrony in follicular wave emergence and subsequent ovulation following dominant follicle ablation or estradiol-17? administration. Six cycling Murrah buffaloes were sequentially allotted to three groups, that is, control, follicular ablation, and estradiol-17? groups. For the control group, buffaloes at random stages of estrous cycle were examined daily by transrectal ultrasonography for 14 days and the day of wave emergence was recorded. Following induced luteolysis and ovulation (Day 0), these buffaloes were included in the ablation group. All follicles (>5mm) were ablated on Day 3 or 5 or 7 (n=2 each day). Seven days after the ablation, these buffaloes were administered prostaglandin F2? to induce luteolysis and ovulation. Following this, buffaloes were included in the estradiol treatment group with estradiol administered on similar days as for ablation in the ablation group. Luteolysis was induced nine days after the estradiol injection. All animals of the treatment groups were subjected to transrectal ultrasound and blood samplings daily from treatment to induced ovulation. The follicular waves emerged significantly earlier (P=0.001) in both the ablation (2.1±0.79 days) and estradiol (4.0±0.25 days) treatment groups than the control group (8.3±0.88 days). The deviation from mean day of ovulation was greater (P=0.02) for the control group buffaloes (1.66±0.3 day) than those of the treatment groups (ablation, 0.76±0.2 and estradiol, 0.58±0.2 day). In conclusion, both ablation and estradiol resulted in synchronous emergence of a new follicular wave irrespective of stage at which the treatment was given, with greater synchrony of ovulations in water buffalo. PMID:24612954

Honparkhe, M; Gandotra, V K; Matharoo, J S; Ghuman, S P S; Dadarwal, D; Singh, Jaswant

2014-04-01

72

Efficacy of PGF 2? to synchronize estrus in water buffalo cows ( Bubalus bubalis) is dependent upon plasma progesterone concentration, corpus luteum size and ovarian follicular status before treatment  

Microsoft Academic Search

This study was conducted to identify factors affecting PGF2? efficacy to synchronize estrus in water buffalo cows. After detection of a corpus luteum (CL) by rectal palpation, cows were treated (im) with dinoprost (12.5, 25 or 50mg) or d(+) cloprostenol (75, 150 or 300?g) in a total of 66 treatments. Blood samples were collected 0, 24 and 48h after treatment

L. F. C Brito; R Satrapa; E. P Marson; J. P Kastelic

2002-01-01

73

Amount of mRNA and localization of vascular endothelial growth factor and its receptors in the ovarian follicle during estrous cycle of water buffalo (Bubalus bubalis).  

PubMed

The objective of the present study was to characterize the temporal patterns of gene expression for vascular endothelial growth factors (VEGF) and VEGF receptors during ovarian follicular growth, development and maturation in buffalo (Bubalus bubalis). Follicles were classified into four groups according to size and the concentration of estradiol-17? (E2) in follicular fluid (FF): Group I (small), 4-6mm diameter, E2>0.5ng/ml of FF; Group II (medium), 7-9mm, E2=0.5-5ng/ml; Group III (large), 10-13mm, E2=5-40ng/ml; Group IV(pre-ovulatory), >13mm, E2>180ng/ml). The mRNAs for FSH receptor (FSHR), LH receptor (LHR) and aromatase (CYP19A1) in theca interna and granulosa layers were also determined, further defining the maturational state of each group. The relative expression of VEGF isoforms (120, 164, and 188 amino acid forms), as determined by quantitative real-time PCR (qRT-PCR), increased during follicular development in both the granulosa (P<0.05) and theca layers. Relative amounts of VEGF receptors (VEGFR-1 and VEGFR-2) were least in granulosa cell (GC) and theca interna cell (TI) layers of Gp-I follicles. The amount of VEGFR-2 transcripts increased in the granulosa layer throughout development, reaching a maximum in Gp-IV follicles (P<0.05). The relative amount of VEGF isoforms and receptors in follicle lysates, as determined by western blotting, increased throughout follicular maturation to maximum amounts in pre-ovulatory follicles. Immunohistochemistry revealed a clear localization of VEGF isoforms and receptors in both steroidogenic cell types (GC and TI) and of VEGF receptors in the vascular endothelial cells of the thecal blood vessels. The most intense immunofluorescence was evident in pre-ovulatory follicles compared to other smaller follicles. These data provide evidence that the VEGF may contribute to the extensive capillary proliferation associated with the increase in size, selection, and maturation of the pre-ovulatory follicle. This may facilitate follicle maturation by enhancing the supply of nutrients, hormones, and other essential blood-borne signals to the follicle. VEGF may also promote maturation of follicles through recently recognized, non-angiogenic mechanisms. PMID:23375984

Babitha, V; Panda, R P; Yadav, V P; Chouhan, V S; Dangi, S S; Khan, F A; Singh, G; Bag, S; Taru Sharma, G; Silvia, W J; Sarkar, M

2013-03-01

74

Expression and localization of vascular endothelial growth factor and its receptors in the corpus luteum during oestrous cycle in water buffaloes (Bubalus bubalis).  

PubMed

The aim of this study was to document the expression and localization of VEGF system comprising of VEGF isoforms (VEGF 120, VEGF 164 and VEGF 188) and their receptors (VEGFR1 and VEGFR2) in buffalo corpus luteum (CL) obtained from different stages of the oestrous cycle. Real-time RT-PCR (qPCR), Western blot and immunohistochemistry were applied to investigate mRNA expression, protein expression and localization of examined factors. In general, all the components of VEGF system (the VEGF isoforms and their receptors) were found in the water buffalo CL during the oestrous cycle. The mRNA as well as protein expression of VEGF system was highest during the early and mid-luteal phase, which later steadily decreased (p < 0.05) after day 10 to reach the lowest level in regressed CL. As demonstrated by immunohistochemistry, VEGF protein was localized predominantly in luteal cells; however, VEGFR1 and VEGFR2 were localized in luteal cells as well as in endothelial cells. In conclusion, the dynamics of expression and localization of VEGF system in buffalo corpora lutea during the luteal phase were demonstrated in this study, indicating the possible role of VEGF system in the regulation of luteal angiogenesis and proliferation of luteal as well as endothelial cells through their non-angiogenic function. PMID:23551326

Chouhan, V S; Panda, R P; Yadav, V P; Babitha, V; Khan, F A; Das, G K; Gupta, M; Dangi, S S; Singh, G; Bag, S; Sharma, G T; Berisha, B; Schams, D; Sarkar, M

2013-10-01

75

Characterization of enterotoxigenic E. coli (ETEC), Shiga-toxin producing E. coli (STEC) and necrotoxigenic E. coli (NTEC) isolated from diarrhoeic Mediterranean water buffalo calves (Bubalus bubalis).  

PubMed

Two hundred and twenty Escherichia coli isolates from 314 Mediterranean water buffalo calves less than 4 weeks old affected by severe diarrhoea with a lethal outcome were characterized for the presence of the virulence factors LT, ST, Stx1, Stx2, haemolysins, intimin, CNF1, CNF2, CDT-I, CDT-II, CDT-III, CDT-IV, and F17-related fimbriae (F17a, F17b, F17c, F17d). The prevalence of ETEC, STEC and NTEC were 1.8%, 6.8% and 20.9%, respectively. The ETEC isolates were all LT-positive and ST-negative. The STEC isolates were all Stx and intimin-positive, with Stx1 (80%) more frequent than Stx2 (27%). The NTEC isolates were all CNF and Hly-positive, with CNF2 (83%) more frequent than CNF1 (22%). Susceptibility assays to 11 antimicrobials displayed high rates of resistance (>30%) to antimicrobials tested. These data show that the most prevalent strains in diarrhoeic water buffalo calves were NTEC, mostly CNF2 and HlyA-positive, with strong associations CNF2/CDT-III and CNF2/F17c. PMID:21658736

Borriello, G; Lucibelli, M G; De Carlo, E; Auriemma, C; Cozza, D; Ascione, G; Scognamiglio, F; Iovane, G; Galiero, G

2012-08-01

76

Association of a novel SNP in exon 10 of the IGF2 gene with growth traits in Egyptian water buffalo (Bubalus bubalis).  

PubMed

Insulin-like growth factor 2 (IGF2) plays an important role in muscle growth and it might be used as a marker for the growth traits selection strategies in farm animals. The objectives of this study were to detect polymorphisms in exon 10 of IGF2 and to determine associations between these polymorphisms and growth traits in Egyptian water buffalo. PCR-single-strand conformation polymorphism (SSCP) and DNA sequencing methods were used to detect any prospective polymorphism. A novel single nucleotide polymorphism (SNP), C287A, was detected. It was a non-synonymous mutation and led to replacement of glutamine (Q) amino acid (aa) by histidine (H) aa. Three different SSCP patterns were observed: AA, AC, and CC, with frequencies of 0.540, 0.325, and 0.135, respectively. Association analyses revealed that the AA individuals had a higher average daily gain (ADG) than other individuals (CC and AC) from birth to 9 months of age. We conclude that the AA genotype in C287A SNP in the exon 10 of the IGF2 gene is associated with the ADG during the age from birth to 9 months and could be used as a potential genetic marker for selection of growth traits in Egyptian buffalo. PMID:24756464

Abo-Al-Ela, Haitham G; El-Magd, Mohammed Abu; El-Nahas, Abeer F; Mansour, Ali A

2014-08-01

77

Post-warming hatching and birth of live calves following transfer of in vitro-derived vitrified water buffalo ( Bubalus bubalis) embryos  

Microsoft Academic Search

Viability of in vitro-derived vitrified-warmed preimplantation stage buffalo embryos were assessed in vitro and in vivo. Oocytes were collected from ovaries of slaughtered riverine buffaloes, matured and fertilized in vitro with frozen semen from riverine buffalo bull and cultured on cumulus cell monolayers. Resultant preimplantation stage embryos were cryopreserved by vitrification with ethylene glycol, ficoll and sucrose. Seventy-one frozen embryos

Danilda Hufana-Duran; Prudencio B Pedro; Hernando V Venturina; Rogelio D Hufana; Apolinario L Salazar; Peregrino G Duran; Libertado C Cruz

2004-01-01

78

The efficacy and safety of alphacypermethrin as a pour-on treatment for water buffalo (Bubalus bubalis) infested with Haematopinus tuberculatus (Phthiraptera: Haematopinidae).  

PubMed

The sucking louse Haematopinus tuberculatus (Burmeister 1839) is an ectoparasite of buffaloes, cattle, camels, and American bison. Alphacypermethrin (ACYP) is a pyrethroid insecticide commonly used to control arthropods of veterinary and public health interest. Therapeutics, such as antiparasitic compounds, is often administered to buffaloes based on dosage and intervals recommended for cattle because very few drugs have buffalo-specific label indications. A trial was conducted on 20 louse-infested buffaloes at a farm to assess the efficacy and safety of ACYP pour-on, at the manufacturer's recommended dose for cattle, on buffaloes naturally infested by H. tuberculatus. Ten animals were assigned to ACYP-treated group (ACYP-group) and ten to untreated control group (C-group). On day 0, all ACYP-group buffaloes received alphacypermethrin pour-on. Louse counts were performed on days -1, 7, 14, 21, 28, 35, 42, 49, and 56 at eight predilection sites on the skin of each buffalo. ACYP was completely effective (100%) at day 7, highly effective (99.8%) at day 14, and completely effective (100%) from day 21 until the end of the study (day 56 post-treatment). During the trial, ACYP was well tolerated by all animals as there were no observed clinically adverse reactions. The results of this trial suggest that ACYP is an effective, safe, and user-friendly compound suitable for treatment of buffaloes with natural louse infestations. PMID:23733232

Veneziano, Vincenzo; Neglia, Gianluca; Cimmino, Roberta; Balestrieri, Anna; Rufrano, Domenico; Bastianetto, Eduardo; Santoro, Mario; Gokbulut, Cengiz

2013-08-01

79

Carcass Characteristics and Meat Quality of Swamp Buffaloes (Bubalus bubalis) Fattened at Different Feeding Intensities  

PubMed Central

Twenty-four male 1-year old swamp buffaloes (Bubalus bubalis) were randomly allocated to 4 groups. One group grazed on guinea grass (GG) and another on guinea grass and the legume Stylosanthes guianensis (GL). The other two groups were kept in pens and fed freshly cut guinea grass and concentrate at an amount of 1.5% (GC1.5) and 2.0% (GC2.0) of body weight, respectively. The effect of the different feeding intensities on carcass characteristics and meat quality were assessed. The mean body weight at slaughter was 398 (±16) kg. Average daily gain was higher in concentrate-supplemented groups (570 and 540 g/d in GC1.5 and GC2.0, respectively) when compared to GG (316 g/d) and GL (354 g/d) (p<0.01). Likewise, the warm carcass weight was higher in GC1.5 and GC2.0 compared to GG and GL. Dressing percentage was 48.1% and 49.5% in GC1.5 and GC2.0 in comparison to 42.9% and 44.8% observed in GG and GL, respectively. Meat of Longissimus throracis from GC1.5 and GC2.0 was redder in color (p<0.01), while water holding capacity (drip and thawing loss) was improved in pasture-fed groups (p<0.05). Protein and fat content of Longissimus thoracis was higher in animals supplemented with concentrate (p<0.01), as was cholesterol content (p<0.05), whereas PUFA:SFA ratio was higher and n-6/n-3 ratio lower (p<0.01) in pasture-fed buffaloes. Results of the present study showed that the supplementation of pasture with concentrate enhances the growth and carcass characteristics of swamp buffaloes expressed in superior dressing percentage, better muscling, and redder meat with a higher content of protein and fat, whereas animals grazing only on pasture had a more favorable fatty acid profile and water holding capacity. In conclusion, the supplementation of concentrate at a rate of about 1.5% of body weight is recommended to improve the performance and carcass quality of buffaloes. PMID:25049987

Lambertz, C.; Panprasert, P.; Holtz, W.; Moors, E.; Jaturasitha, S.; Wicke, M.; Gauly, M.

2014-01-01

80

Carcass Characteristics and Meat Quality of Swamp Buffaloes (Bubalus bubalis) Fattened at Different Feeding Intensities.  

PubMed

Twenty-four male 1-year old swamp buffaloes (Bubalus bubalis) were randomly allocated to 4 groups. One group grazed on guinea grass (GG) and another on guinea grass and the legume Stylosanthes guianensis (GL). The other two groups were kept in pens and fed freshly cut guinea grass and concentrate at an amount of 1.5% (GC1.5) and 2.0% (GC2.0) of body weight, respectively. The effect of the different feeding intensities on carcass characteristics and meat quality were assessed. The mean body weight at slaughter was 398 (±16) kg. Average daily gain was higher in concentrate-supplemented groups (570 and 540 g/d in GC1.5 and GC2.0, respectively) when compared to GG (316 g/d) and GL (354 g/d) (p<0.01). Likewise, the warm carcass weight was higher in GC1.5 and GC2.0 compared to GG and GL. Dressing percentage was 48.1% and 49.5% in GC1.5 and GC2.0 in comparison to 42.9% and 44.8% observed in GG and GL, respectively. Meat of Longissimus throracis from GC1.5 and GC2.0 was redder in color (p<0.01), while water holding capacity (drip and thawing loss) was improved in pasture-fed groups (p<0.05). Protein and fat content of Longissimus thoracis was higher in animals supplemented with concentrate (p<0.01), as was cholesterol content (p<0.05), whereas PUFA:SFA ratio was higher and n-6/n-3 ratio lower (p<0.01) in pasture-fed buffaloes. Results of the present study showed that the supplementation of pasture with concentrate enhances the growth and carcass characteristics of swamp buffaloes expressed in superior dressing percentage, better muscling, and redder meat with a higher content of protein and fat, whereas animals grazing only on pasture had a more favorable fatty acid profile and water holding capacity. In conclusion, the supplementation of concentrate at a rate of about 1.5% of body weight is recommended to improve the performance and carcass quality of buffaloes. PMID:25049987

Lambertz, C; Panprasert, P; Holtz, W; Moors, E; Jaturasitha, S; Wicke, M; Gauly, M

2014-04-01

81

Bovine papillomavirus type 2 infects the urinary bladder of water buffalo (Bubalus bubalis) and plays a crucial role in bubaline urothelial carcinogenesis.  

PubMed

Bovine papillomavirus type 2 (BPV-2) has been shown to infect and play a role in urinary bladder carcinogenesis of buffaloes grazed on pastures with ferns from the Marmara and Black Sea Regions of Turkey. BPV-2 DNA has been found in both neoplastic and non-neoplastic lesions of the urinary bladder. Furthermore, this virus may be a normal inhabitant of the urinary bladder since BPV-2 DNA has also been detected in clinically normal buffaloes. The viral activation by fern immunosuppressant or carcinogen may trigger the urothelial cell transformation. The E5 oncoprotein was solely detected in urothelial tumours and appeared to be co-localized with the overexpressed and phosphorylated platelet derived growth factor (PDGF) ? receptor in a double-colour immunofluorescence assay. Our results indicate that the E5-PDGF ? receptor interaction also occurs in spontaneous tumours of the bubaline urinary bladder, revealing an additional role of BPV-2 in bladder carcinogenesis of buffaloes. PMID:23100367

Roperto, Sante; Russo, Valeria; Ozkul, Ayhan; Sepici-Dincel, Aylin; Maiolino, Paola; Borzacchiello, Giuseppe; Marcus, Ioan; Esposito, Iolanda; Riccardi, Marita Georgia; Roperto, Franco

2013-02-01

82

Gene mapping in the river buffalo (Bubalus bubalis L)  

E-print Network

/borate/EDTA (ethylenediaminetetraacetic acid) system, pH 8.6, (Shows et al, 1964) for LDH, ME and SOD, and a Tris/citrate system, pH 6 in the buffalo. Parental cells were blood lymphocytes from two Egyptian river buffaloes, and cells of the HPRT. Abbreviations and criteria for homology are in agreement with the ISGN system (Human Gene Mapping 5, 1979

Boyer, Edmond

83

Effects of Smallmouth Buffalo and Potassium Permanganate Treatment on Plankton and Pond Water Quality  

Microsoft Academic Search

There is recent interest in using smallmouth buffalo Ictiobus bubalus and treatment with potassium permanganate (KMnO4) to control Dero digitata, the aquatic oligochaete host involved in the life cycle of proliferative gill disease. To complement those investigations, we determined the changes in water quality, phytoplankton, and zooplankton resulting from KMnO4 treatment and stocking of smallmouth buffalo into nursery ponds for

Charles C. Mischke; David J. Wise; James A. Steeby; Paul V. Zimba

2006-01-01

84

Sequence characterization and comparative analysis of the gastrotropin gene in buffalo (Bubalus bubalis).  

PubMed

In this study, we compared the complete sequence of the FABP6 gene from an animal representing the Murrah breed of the river buffalo (Bubalus bubalis) with the gene sequence from different mammals. The buffalo FABP6 gene is 6105 bp in length and is organized into four exons (67, 176, 90, and 54 bp), three introns (1167, 1737, and 2649 bp), a 5?UTR (93 bp), and a 3?UTR (72 bp). A total of 22 repetitive elements were identified at the intronic level, and four of these (L1MC, L1M5, MIRb, and Charlie4z) were identified as being exclusive to buffalo. Comparative analysis between the FABP6 gene coding sequence and the amino acid sequence with its homologues from other mammalian species showed a percentage of identity varying from 79 to 98% at the DNA coding level and 70 to 96% at the amino acid level. In addition, the alignment of the gene sequence between the Murrah and the Mediterranean breeds revealed 20 potential single nucleotide polymorphisms, which could be candidates for validation in commercial buffalo populations. PMID:25526214

Stafuzza, N B; Borges, M M; Amaral-Trusty, M E J

2014-01-01

85

Morphogenesis of Mammary Glands in Buffalo (Bubalus bubalis).  

PubMed

The present research was elucidated on the morphogenesis of mammary gland of buffalo during prenatal development. Total of 16 foetuses ranging from 1.2?cm (34 days) to 108?cm CVRL (curved crown rump length) (317 days) were used for study. The study revealed that mammary line was first observed at 1.2?cm CVRL (34 days), mammary hillock at 1.7?cm (37 days), and mammary bud at 2.6 cm CVRL (41 days) foetuses. Epidermal cone was found at 6.7?cm CVRL (58 days) whereas primary and secondary ducts were observed at 7.4?cm CVRL (62 days) and 15?cm CVRL (96 days), respectively. Connective tissue whorls were reported at 18.2 cm CVRL (110 days) and internal elastic lamina and muscle layers at 24.1?cm CVRL (129 days). Lobules were observed at 29.3 cm CVRL (140 days), rosette of furstenberg at 39.5 cm CVRL (163 days), and keratin plug at 45.5?cm CVRL (176 days) foetus. Primordia of sweat and sebaceous glands around hair follicle were seen at 21.2?cm CVRL (122 days) of foetal life. Differentiation of all the skin layers along with cornification was observed at 69?cm (229 days) in group III foetuses. PMID:24876967

Challana, Amit; Gupta, Anuradha; Bansal, Neelam; Uppal, Varinder

2014-01-01

86

RsaI repetitive DNA in Buffalo Bubalus bubalis representing retrotransposons, conserved in bovids, are part of the functional genes  

PubMed Central

Background Repetitive sequences are the major components of the eukaryotic genomes. Association of these repeats with transcribing sequences and their regulation in buffalo Bubalus bubalis has remained largely unresolved. Results We cloned and sequenced RsaI repeat fragments pDp1, pDp2, pDp3, pDp4 of 1331, 651, 603 and 339 base pairs, respectively from the buffalo, Bubalus bubalis. Upon characterization, these fragments were found to represent retrotransposons and part of some functional genes. The resultant clones showed cross hybridization only with buffalo, cattle, goat and sheep genomic DNA. Real Time PCR, detected ~2 × 104 copies of pDp1, ~ 3000 copies of pDp2 and pDp3 and ~ 1000 of pDp4 in buffalo, cattle, goat and sheep genomes, respectively. RsaI repeats are transcriptionally active in somatic tissues and spermatozoa. Accordingly, pDp1 showed maximum expression in lung, pDp2 and pDp3 both in Kidney, and pDp4 in ovary. Fluorescence in situ hybridization showed repeats to be distributed all across the chromosomes. Conclusions The data suggest that RsaI repeats have been incorporated into the exonic regions of various transcribing genes, possibly contributing towards the architecture and evolution of the buffalo and related genomes. Prospects of our present work in the context of comparative and functional genomics are highlighted. PMID:21718551

2011-01-01

87

Effect of Leptin on In Vitro Nuclear Maturation and Apoptosis of Buffalo (Bubalus bubalis) Oocyte  

PubMed Central

Background: Leptin, as a 16 kDa adipokine, is a pleiotropic cytokine-like hormone that primarily secreted from adipose tissue. It also involves in the regulation of energy homeostasis, neuroendocrine function, immunity, lipid and glucose homeostasis, fatty acid oxidation, angiogenesis, puberty and reproduction. The aim of this study was to investigate the effects of in vitro addition of leptin to in vitro maturation (IVM) medium on buffalo oocyte maturation and apoptosis. Materials and Methods: In this experimental study, Ovaries from apparently normal reproductive organs of slaughtered adult buffaloes (Bubalus bubalis) with unknown breeding history were collected from Urmia Abattoir, Urmia, Iran, and were transported immediately to the laboratory in a thermos flask containing sterile normal saline with added antibiotics. Oocytes were aspirated from 2-8 mm visible follicles of the ovaries using an 18-G needle attached to a 10 ml syringe. IVM medium included tissue culture medium-199 (TCM-199), 10% fetal bovine serum (FBS), 22 µg/ml sodium pyruvate, 0.5 IU/ml ovine follicle-stimulating hormone (oFSH), 0.5 IU/ml ovine luteinizing hormone (oLH), 1 ?g/ml oestradiol, 50 ?g/ml gentamycin, and leptin [0 (control), 10, 50, and 100 ng/ml]. The good quality buffalo oocytes (batches of 10 oocytes) were placed in a culture plate containing six 50 ?l droplets of maturation medium, covered with sterilized mineral oil, and then incubated at 38.5?C with 5% CO2 in air for 24 hours. The maturation of oocytes was evaluated under a stereomicroscope by detecting the first polar body extrusion of oocytes. FITC-Annexin V propidium iodide (PI) staining method was used to detect oocyte apoptosis. Results: From a total of 115 collected ovaries, 1100 oocytes were recovered among which 283 oocyte were suitable for IVM. In the groups of leptin treated with 0 (control), 10, 50 and 100 ng/ml, the percentage of oocytes maturation was 74.65, 83.81, 77.85, and 75.40%, while the percentage of oocytes apoptosis was 9.83, 9.54, 9.93, and 10.42%, respectively. Our results showed that addition of 10 ng/ml leptin to buffalo IVM medium increased oocyte maturation, significantly, as compared with that in control group. However, addition of leptin to IVM medium had no significant influence on buffalo oocyte apoptosis. Conclusion: Our findings suggested that addition of 10 ng/ml leptin to IVM medium of buffalo oocyte can improve oocyte nuclear maturation. Furthermore, we showed that there is no relation between in vitro addition of leptin to buffalo oocyte IVM medium and oocyte apoptos Conclusion: Our findings suggested that addition of 10 ng/ml leptin to IVM medium of buffalo oocyte can improve oocyte nuclear maturation. Furthermore, we showed that there is no relation between in vitro addition of leptin to buffalo oocyte IVM medium and oocyte apoptosis. PMID:24696768

Khaki1, Amir; Batavani, Rouzali; Najafi, Gholamreza; Tahmasbian, Hamid; Belbasi, Abolfazl; Mokarizadeh, Aram

2014-01-01

88

Comparative therapeutic effect of toltrazuril, sulphadimidine and amprolium on Eimeria bovis and Eimeria zuernii given at different times following infection in buffalo calves ( Bubalus bubalis)  

Microsoft Academic Search

We compared the therapeutic effect of three anticoccidial drugs (toltrazuril, sulphadimidine and amprolium) in buffalo (Bubalus bubalis) calves experimentally infected with Eimeria bovis (E. bovis) and E. zuernii oocysts (3×104oocyst\\/calf). Buffalo calves (1.5–4 month old, 70-kg body weight) were randomly allocated into 3 groups (9 calves each). Group T was experimentally infected with oocysts and treated with toltrazuril (20mg\\/kg BW

Mohamed M. Ghanem; Mervat E. Radwaan; Abdel Moneim M. Moustafa; Mohamed H. Ebeid

2008-01-01

89

Histology and Ultrastructure of the Lymph Nodes of the Buffalo (Bos bubalus).  

PubMed

Pre-scapular, femoral and mesenteric lymph nodes from five buffalo calves and five buffalo bulls were studied using light and transmission electron microscopy. The nodes were surrounded with a thin capsule of dense connective tissue and smooth muscles. Subcapsular and trabecular lymphatic sinuses were lined with endothelial cells resting on a basement membrane. The cortex was formed by lymphoid follicles and inter-follicular lymphocytes. Primary and secondary follicles were observed. The medulla was made up of medullary cords of lymphocytes separated by lymphatic sinuses. These sinuses were lined with a discontinuous epithelium and interestingly crossed by reticular fibres. High endothelial venules were found in the paracortical area. Several lymphocytes were observed infiltrating the wall of these venules. The lymph nodes of the Egyptian water buffalo showed a typical structure compared with the majority of mammals, with no age-related structural variation. PMID:24810142

Zidan, M; Pabst, R

2014-05-01

90

Hydatidosis and Hepatic Trematodosis in Water Buffaloes in Khuzestan Province,  

E-print Network

Abstract: Meat inspection records in the‘‘Complex abattoir’ ’ located in Ahwaz, a city in southwest Iran and the capital of Khuzestan province, during a 10- year period (1998-2008) were used to determine the prevalence and long-term trend of cystic echinococcosis and hepatic trematodosis in water buffaloes (Bubalus bubalis) in the region. A total of 57,555 water buffaloes were slaughtered in the 10-year period and overall 13,619 (23.66%) livers and 20105 (34.93%) lungs were condemned in this ruminant. Studied zoonotic helminthosis were responsible for 78.04 % of total liver condemnations of which 55.32%, 0.74 % and 21.98 were due to fasciolosis, dicrocoeliosis and hydatidosis, respectively. Pulmonary hydatidosis infection rate was significantly higher than hepatic hydatidosis in slaughtered buffaloes. The overall trend showed a considerable decline in the prevalence of the mentioned zoonotic helminthosis over the study period which was more remarkable in the first several years. Hence, the prevalence of fasciolosis, dicrocoeliosis and hydatidosis from 24.09, 0.23 and 32.51 % in 1998-1999 decreased to 10.12, 0.02 and 10.95 % in 2007-2008 for water buffaloes, respectively. There were no significant variations due to prevalence of these infections during seasonal patterns probably due to chronicity of the diseases. The study has established that fasciolosis and hydatidosis are prevalent in this animal slaughtered at Ahwaz abattoir and cause higher condemnation rates of edible offals. The findings merit for more extensive epidemiological investigations of the zoonotic infections in water buffaloes to determine the prevalence, economic impact and public health importance for the diseases in the region better.

Nayeb Ali

91

Effect of bypass fat supplementation on productive performance and blood biochemical profile in lactating Murrah (Bubalus bubalis) buffaloes.  

PubMed

The study investigated the effect of dietary supplementation of bypass fat on productive performance and blood biochemical profile of lactating Murrah buffaloes (Bubalus bubalis). Fifteen multiparous buffaloes (2-4 lactation) of early to mid lactation were divided in three homogenous groups T(1) (control), T(2), and T(3) of five each. The animals in T(1) were fed with a basal diet consisting of a concentrate mixture, green sorghum, and wheat straw as per requirements, while the animals in group T(2) and T(3) were fed with same ration supplemented with 0.7 % (100 g/day) and 1.4 % (200 g/day) bypass fat (on dry matter intake (DMI) basis), respectively. The feed intake, milk yield, and milk composition were not influenced by supplemental bypass fat. However, fat-corrected milk (6.5 %) yield was higher (P < 0.05) in T(3) (14.21) than that of T(1) (9.83) and similar with T(2) (11.05). Feed efficiency (milk yield/kg DMI) was higher (P < 0.05) in group T(3) (0.51) than that of T(1) (0.38) and T(2) (0.41) indicating that buffaloes fed with bypass fat which is 1.4 % (200 g/day) of the diet were economically more efficient. The serum cholesterol level was higher (P < 0.01) in bypass fat-supplemented group (T(2) and T(3)) of animals. Serum high density lipoprotein (HDL) cholesterol (good cholesterol) level was more (P < 0.05) than LDL cholesterol (bad cholesterol) level with higher dose of bypass fat in T(3) than T(2). It was concluded that bypass fat supplementation with 1.4 % of the diet (200 g/day) increased the fat-corrected milk production and feed efficiency along with serum HDL cholesterol level in lactating Murrah buffaloes. PMID:22373930

Ranjan, Amit; Sahoo, Biswanath; Singh, Vijay Kumar; Srivastava, Susant; Singh, Suresh Pratap; Pattanaik, Ashok Kumar

2012-10-01

92

Identification of three novel myeloid cathelicidin cDNAs and their predicted peptides in buffalo (Bubalus bubalis).  

PubMed

Antimicrobial peptides (AMPs) are broad spectrum antibiotics, which mostly act without specific receptors. Identification of AMPs is important in the current scenario of emerging multi-drug resistant bacteria. In the present study, in an attempt to identify new AMPs, myeloid cathelicidin cDNAs were synthesized from buffalo (Bubalus bubalis) bone marrow and were amplified using specific primers. Sequence analysis of cloned cDNAs revealed three novel myeloid cathelicidins. They were named based on the number of active amino acids in the C-terminal region of their predicted peptide sequences as BuMAP-28 (having an additional Gly at position 22nd), BuMAP-29 (having an additional IIe at position 27) and BuMAP-34, compared to BMAP-27, BMAP-28 and BMAP-34 of cattle. The BuMAPs showed 93%, 95% and 87% homology respectively with that of its cattle counterpart. Predicted number of amino acids of the cDNAs was 159, 155 and 157 residues, with cationic C-terminal sequences of 28, 29 and 34, respectively, which correspond to putative antimicrobial domains. Several amino acid substitutions were observed in all the three cathelicidins. The conformation of the peptides was predicted to be alpha helical, having total net positive charge and hydrophobicity, similar to that of BMAPs in cattle. Comparative analysis of the predicted peptides suggested potential antimicrobial activity and the sequence variations detected might enable the peptides to act as effective broad spectrum antimicrobial agents. PMID:24772945

Panicker, Varuna P; George, Sisilamma

2013-08-01

93

Myxosporeans infecting the gills of bigmouth buffalo (Ictiobus bubalus) in Illinois, USA.  

PubMed

Four myxosporean species were found on the gills of Ictiobus bubalus from Illinois (USA). Myxobolus endovasus (Davis, 1947) Grinham et Cone, 1990 is revised. Three new species are recorded. Myxobolus enoblei sp. n. has spores ovoid in frontal view, 14.3 x 13 microns in size. Myxobolus morrisonae sp. n. has spores subcircular in frontal view, 10 x 9.5 microns in size; the surface of shell valves appears hairy when studied by SEM. Triangula illinoisensis sp. n. has spores rounded semicircular in frontal view, 10.2 x 12.8 microns in size. Triangula illinoisensis is the fourth species of its genus to be described from fishes. PMID:8682408

Lom, J; Cone, D

1996-01-01

94

Novel polymorphisms of the IGF1R gene and their association with average daily gain in Egyptian buffalo (Bubalus bubalis).  

PubMed

The objective of this study was to detect insulin-like growth factor 1 receptor (IGF1R) polymorphisms, their allele, and genotype frequencies and to determine associations between these polymorphisms and growth traits in Egyptian water buffalo. Three loci of the IGF1R coding region were amplified by RT-PCR and, subsequently, subjected to sequence analysis, followed by single-strand conformation polymorphism to identify different allelic patterns. A total of 11 novel polymorphisms were detected; 6 SNPs among Egyptian water buffaloes and 5 polymorphisms compared with Indian buffalo (Y12700). Three of those polymorphisms; GAG Indel polymorphism, C261G, and G263C SNPs, were nonsynonymous mutations. The GAG Indel polymorphism led to deletion of E (glutamic) amino acid (aa) in the IGF1R of Egyptian water buffaloes compared with Indian buffalo. However, C261G SNP, which replaced A (alanine) by G (glycine) aa, and G263C SNP, which changed A (alanine) to P (proline) aa, were detected among Egyptian water buffaloes. Three different single-strand conformation polymorphism patterns were observed in exon 21: CC/CC, GG/GG, and CG/GC with frequencies of 0.291, 0.253, and 0.556, respectively. The heterozygous animals (CG/GC) had a higher ADG than homozygous animals (CC/CC and GG/GG) from birth to 6 mo of age. We conclude that the heterozygous haplotype, C261G/G263C, in exon 21 of the IGF1R gene is associated with the ADG during the early stages of life (from birth to 6 mo of age) and could be used as a genetic marker for selection of growth traits in Egyptian buffalo. PMID:23820243

El-Magd, M A; Abbas, H E; El-kattawy, A M; Mokhbatly, A

2013-08-01

95

Microscopical and serological studies on Sarcocystis infection with first report of S. cruzi in buffaloes (Bubalus bubalis) in Assiut, Egypt.  

PubMed

This study was performed for the purpose of investigating the prevalence and the species composition of Sarcocystis spp. in buffaloes in Assiut province, Egypt. Macroscopically we reported the infection of buffaloes with Sarcocystis fusiformis, while microscopically three Sarcocystis species (Sarcocystis cruzi, Sarcocystis levinei and Sarcocystis hominis) cysts were recognized, and were differentiated by their morphological features using both histopathological sections and electron microscope scanning. Regarding the prevalence of Sarcocystis species among buffaloes in Assiut province, we reported that, using gross examination of 90 buffaloes' esophagus, only 23 samples out of 90 (25.5 %) were found to be infected; on the other hand, by using microscopical examination, the prevalence was 27.7 % (25 samples out of 90 samples were found to be infected). Using ELISA, 85 samples out of 90 (94.4 %) were found positive, an overall prevalence of 94.4 %. In this work we concluded that customary meat inspection methods in abattoirs in Egypt are insufficient for detecting Sarcocystis infection. Due to the presence of hidden or microscopic cysts, we strongly recommend the use of combined microscopical examination and ELISA for Sarcocystis diagnosis, to avoid human infection of such zoonotic parasite and to control the consequent disease. In addition, this study introduced the first report of S. cruzi in buffaloes in Egypt, and proved the hypothesis that S. cruzi is able to use animals such as water buffalo as intermediate hosts. PMID:25320487

Metwally, Asmaa M; Abd Ellah, Mahmoud R; Al-Hosary, Amira A; Omar, Mosaab A

2014-12-01

96

Laboratory-based measurements of swimming performance and related metabolic rates of field-sampled smallmouth buffalo (Ictiobus bubalus): a study of seasonal changes.  

PubMed

Numerous studies have demonstrated how the performance physiology of fish may change when they are acclimated to designated laboratory temperatures, but few researchers have examined naturally occurring seasonal effects on several physiological parameters associated with swimming performance. Using field-acclimatized smallmouth buffalo (Ictiobus bubalus) collected each season, we report significant seasonal effects in the following variables: critical swimming speed (modified), metabolic rate (standard, active, and scope for activity), and swimming efficiency (total and net cost of transport). Underlying seasonal changes in performance was the reproductive cycle of buffalo, particularly the period of fall gonadal recrudescence. Compared with spring, fall buffalo had a significantly lower mean critical swimming speed (72%) and lower active metabolic rate (53%), even when tested at similar temperatures. During spring, buffalo had a high mean critical swimming speed and low net cost of transport in comparison with other seasons. Buffalo are known to participate in a spring migration and spawning that may require the increased performance and efficiency observed during that season. In addition, significant sex effects were detected in winter measurements of standard metabolic rate and net cost of transport, with females the more efficient swimmers. PMID:9678496

Adams, S R; Parsons, G R

1998-01-01

97

Effect of cold shock on enzyme release in buffalo (Bubalus bubalis) spermatozoa  

E-print Network

release in buffalo spermatozoa, i.e. alcohol, malic, glucose-6-phosphate, isocitric, lactate, sorbital, arylsulphatase A and arylsulphatase B. Significant amounts of alcohol, malic and sorbital dehydrogenases, GOT release of hyaluronidase), but low enough to check their denaturation. The dehydrogenases were estimated

Paris-Sud XI, Université de

98

Collagen-IV supported embryoid bodies formation and differentiation from buffalo (Bubalus bubalis) embryonic stem cells  

SciTech Connect

Graphical abstract: EBs formation, characterization and expression of germinal layers marker genes of in vivo developed teratoma using four different types of extracellular matrices. Highlights: Black-Right-Pointing-Pointer Collagen-IV matrix is found cytocompatible for EBs formation and differentiation. Black-Right-Pointing-Pointer Established 3D microenvironment for ES cells development and differentiation into three germ layers. Black-Right-Pointing-Pointer Collagen-IV may be useful as promising candidate for ES cells based therapeutic applications. -- Abstract: Embryoid bodies (EBs) are used as in vitro model to study early extraembryonic tissue formation and differentiation. In this study, a novel method using three dimensional extracellular matrices for in vitro generation of EBs from buffalo embryonic stem (ES) cells and its differentiation potential by teratoma formation was successfully established. In vitro derived inner cell masses (ICMs) of hatched buffalo blastocyst were cultured on buffalo fetal fibroblast feeder layer for primary cell colony formation. For generation of EBs, pluripotent ES cells were seeded onto four different types of extracellular matrices viz; collagen-IV, laminin, fibronectin and matrigel using undifferentiating ES cell culture medium. After 5 days of culture, ESCs gradually grew into aggregates and formed simple EBs having circular structures. Twenty-six days later, they formed cystic EBs over collagen matrix with higher EBs formation and greater proliferation rate as compared to other extracellular matrices. Studies involving histological observations, fluorescence microscopy and RT-PCR analysis of the in vivo developed teratoma revealed that presence of all the three germ layer derivatives viz. ectoderm (NCAM), mesoderm (Flk-1) and endoderm (AFP). In conclusion, the method described here demonstrates a simple and cost-effective way of generating EBs from buffalo ES cells. Collagen-IV matrix was found cytocompatible as it supported buffalo EBs formation, their subsequent differentiation could prove to be useful as promising candidate for ES cells based therapeutic applications.

Taru Sharma, G., E-mail: gts553@gmail.com [Reproductive Physiology Laboratory, Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P. (India); Dubey, Pawan K.; Verma, Om Prakash; Pratheesh, M.D.; Nath, Amar; Sai Kumar, G. [Reproductive Physiology Laboratory, Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P. (India)] [Reproductive Physiology Laboratory, Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar-243 122, Bareilly, U.P. (India)

2012-08-03

99

Seasonal variations in developmental competence and relative abundance of gene transcripts in buffalo (Bubalus bubalis) oocytes.  

PubMed

Hot season is a major constraint to production and reproduction in buffaloes. The present work aimed to investigate the effect of season on ovarian function, developmental competence, and the relative abundance of gene expression in buffalo oocytes. Three experiments were conducted. In experiment 1, pairs of buffalo ovaries were collected during cold season (CS, autumn and winter) and hot season (HS, spring and summer), and the number of antral follicles was recorded. Cumulus oocyte complexes (COCs) were aspirated and evaluated according to their morphology into four Grades. In experiment 2, Grade A and B COCs collected during CS and HS were in vitro matured (IVM) for 24 hours under standard conditions at 38.5 °C in a humidified air of 5% CO2. After IVM, cumulus cells were removed and oocytes were fixed, stained with 1% aceto-orcein, and evaluated for nuclear configuration. In vitro matured buffalo oocytes harvested during CS or HS were in vitro fertilized (IVF) using frozen-thawed buffalo semen and cultured in vitro to the blastocyst stage. In experiment 3, buffalo COCs and in vitro matured oocytes were collected during CS and HS, and then snap frozen in liquid nitrogen for gene expression analysis. Total RNA was extracted from COCs and in vitro matured oocytes, and complementary DNA was synthesized; quantitative Reverse Transcription-Polymerase Chain Reaction was performed for eight candidate genes including GAPDH, ACTB, B2M, GDF9, BMP15, HSP70, and SOD2. The results indicated that HS significantly (P < 0.01) decreased the number of antral follicles and the number of COCs recovered per ovary. The number of Grade A, B, and C COCs was lower (P < 0.05) during HS than CS. In vitro maturation of buffalo oocytes during HS significantly (P < 0.01) reduced the number of oocytes reaching the metaphase II stage and increased the percentage of degenerated oocytes compared with CS. Oocytes collected during HS also showed signs of cytoplasmic degeneration. After IVF, cleavage rate was lower (P < 0.01) for oocytes collected during HS, and the percentage of oocytes arrested at the two-cell stage was higher (P < 0.01) than oocytes IVF during CS. Oocytes matured during CS showed a higher (P < 0.01) blastocyst rate than those matured during HS. Also, COCs recovered in HS showed significant (P < 0.05) upregulation of HSP70 mRNA expression compared with those recovered in CS. For in vitro matured oocytes, CS down regulated the transcript abundance of ACTB and upregulated GAPDH and HSP70 mRNA levels compared with HS condition. In conclusion, HS could impair buffalo fertility by reducing the number of antral follicles and oocyte quality. In vitro maturation of buffalo oocytes during HS impairs their nuclear and cytoplasmic maturation, fertilization, and subsequent embryo development to the morula and blastocyst stages. This could be in part because of the altered gene expression found in COCs and in vitro matured oocytes. PMID:25156970

Abdoon, Ahmed S; Gabler, Christoph; Holder, Christoph; Kandil, Omaima M; Einspanier, Ralf

2014-11-01

100

Plasma hormonal and electrolyte alterations in cycling buffaloes ( Bubalus bubalis) during hot summer months  

NASA Astrophysics Data System (ADS)

Plasma levels of progesterone, prolactin, luteinizing hormone, and electrolytes were monitored by radioimmunoassay in ten cycling buffaloes maintained at Punjab Agricultural University, Ludhiana during the hot summer months of June July. The plasma progesterone concentration ranged from 0.28±0.04 to 3.09±0.03 ng/ml at various stages of the oestrous cycle. Prolactin values ranged from 319±23 to 371±25 ng/ml and LH levels from 0.95±0.05 to 1.35±0.08 ng/ml. Concentrations differed significantly ( P?0.05) at various stages of the cycle. Levels of electrolytes, viz. Ca+ +, Na+ and K+, were well within the normal range. The high levels of prolactin, progesterone and LH during the hot summer were assessed in relation to poor reproductive efficiency in buffaloes.

Singh, Narinder; Chaudhary, K. C.

1992-09-01

101

Reproductive characteristics and thyroidal function in relation with season in Khuzestan buffalo (Bubalus bubalis) bulls  

PubMed Central

High ambient temperature is the major constraint on Buffalo productivity. The aim of this study was to evaluate the reproductive performance and thyroid gland function in winter and summer seasons in Khuzestan buffalo bulls. Six male indigenous buffaloes of Khuzestan with nearly the same age (2-3 years old) and weight were used. Semen and blood samples through jugular vein were collected, every two weeks throughout the summer and winter seasons. The thyroid hormones and thyrotropin stimulating hormone (TSH) concentration in blood serum were measured by radioimmunoassay method. Semen quality was determined, using computer assisted sperm analyzer (CASA) and routine methods. The concentration of thyroxin (T4) was lower in winter than summer (p ? 0.05). The level of T3 uptake was higher in cold season than that of in hot season (p ? 0.05). The differences of tri-iodotyronine (T3) and TSH concentrations, as well as free thyroxin index were not significant between seasons. The semen volume and spermatozoa parameters including concentration, progressive motility, linear velocity, mean velocity, beat cross frequency, linear coefficient and straightness coefficient were higher in winter than summer (p ? 0.05). Semen pH and amplitude of lateral head displacement of spermatozoa were higher in summer than winter (p ? 0.05). In winter, there was positive correlation between spermatozoa concentration and T3 value of blood serum (p ? 0.05). There were positive correlations between values of semen volume and T4, progressive spermatozoa motility percent and TSH, as well as, total motility of spermatozoa and TSH in summer (p ? 0.05). In general, thyroid function and semen quality of Khuzestan buffaloes may be affected by seasons.

Mayahi, Sadegh; Mamouei, Morteza; Tabatabaei, Saleh; Mirzadeh, Khalil

2014-01-01

102

Molecular Identification of Methanogenic Archaea From Surti Buffaloes (Bubalus Bubalis), Reveals More Hydrogenotrophic Methanogens Phylotypes  

PubMed Central

Methane emissions from ruminant livestock are considered to be one of the more potent forms of greenhouses gases contributing to global warming. Many strategies to reduce emissions are targeting the methanogens that inhabit the rumen, but such an approach can only be successful if it targets all the major groups of ruminant methanogens. Therefore, a thorough knowledge of the diversity of these microbes in breeds of buffaloes, as well as in response to geographical location and different diets, is required. Therefore, molecular diversity of rumen methanogens in Surti buffaloes was investigated using 16S rRNA gene libraries prepared from pooled rumen contents from three Surti buffaloes. A total of 171 clones were identified revealing 23 different sequences (phylotypes). Of these 23 sequences, twelve sequences (12 OTUs, 83 clones) and 10 sequences (10 OTUs, 83 clones) were similar to methanogens belonging to the orders Methanomicrobiales and Methanobacteriales, and the remaining 1 phylotype (5 clones) were similar to Methanosarcina barkeri. These unique sequences clustered within a distinct and strongly supported phylogenetic group. Further studies and effective strategies can be made to inhibit the growth of Methanomicrobiales and Methanobacteriales phylotypes to reduce the methane emission from rumen and thus help in preventing global warming. PMID:24031614

Singh, K.M.; Pandya, P.R.; Parnerkar, S.; Tripathi, A.K.; Rank, D.N.; Kothari, R.K.; Joshi, C.G.

2011-01-01

103

In vivo differentiation potential of buffalo (Bubalus bubalis) embryonic stem cell.  

PubMed

Embryonic stem cells (ESCs) derived from inner cell mass (ICM) of mammalian blastocyst are having indefinite proliferation and differentiation capability for any type of cell lineages. In the present study, ICMs of in vitro-derived buffalo blastocysts were cultured into two different culture systems using buffalo fetal fibroblast as somatic cell support and Matrigel as synthetic support to obtain pluripotent buffalo embryonic stem cell (buESC) colonies. Pluripotency of the ESCs were characterised through pluripotency markers whereas, their differentiation capability was assessed by teratoma assay using immuno-compromised mice. Cumulus ooccyte complexes from slaughter house-derived ovaries were subjected to in vitro maturation, in vitro fertilization and in vitro culture to generate blastocysts. Total 262 blastocysts were derived through IVEP with 11.83 % (31/262) hatching rate. To generate buESCs, 15 ICMs from hatched blastocysts were cultured on mitomycin-C-treated homologous fetal fibroblast feeder layer, whereas the leftover 16 ICMs were cultured on extra-cellular matrix (Matrigel). No significant differences were observed for primary ESCs colony formation between two culture systems. Primary colonies as well as passaged ESCs were characterised by alkaline phosphatase staining, karyotyping and expression of transcription-based stem cell markers, OCT-4 and cell surface antigens SSEA-4 and TRA-1-60. Batch of ESCs found positive for pluripotency markers and showing normal karyotype after fifteenth passage were inoculated into eight immuno-compromised mice through subcutaneous and intramuscular route. Subcutaneous route of inoculation was found to be better than intramuscular route. Developed teratomas were excised surgically and subjected to histological analysis. Histological findings revealed presence of all the three germinal layer derivatives in teratoma sections. Presence of germinal layer derivatives were further confirmed by reverse transcriptase-polymerase chain reaction for the presence of differentiation markers like nerve cell adhesion molecule, fetal liver kinase-1 and alpha-feto protein for ectoderm, mesoderm and endoderm, respectively. PMID:22678753

Verma, Om Prakash; Kumar, Rajesh; Nath, Amar; Sharma, Manjinder; Dubey, Pawan Kumar; Kumar, G Sai; Sharma, G Taru

2012-06-01

104

The effects of high temperature and roof modification on physiological responses of swamp buffalo ( Bubalus bubalis) in the tropics  

NASA Astrophysics Data System (ADS)

The objective of the experiments reported here was to measure the effects of cooling techniques (Modified roof vs Normal roof) on the performance and physiology of 12 young male buffaloes with a similar live weight of 160 kg. The study was conducted at Chainat Agriculture and Technology College, Chainat Province, Thailand. The animals were divided randomly into two groups, each group comprising six buffaloes, and the two groups were studied to evaluate the effects of modified roofing (normal roof fitted with woven polypropylene shade cloth) on the subjects' physiological responses to heat stress under hot humid conditions. The modified roof resulted in lowered heat stress in buffaloes compared to those under a standard roof. The difference was shown by the buffaloes having a significantly lower mean rectal temperature (39.14 ± 0.07 vs 40.00 ± 0.10°C) and plasma cortisol (2.14 ± 0.24 vs 3.38 ± 0.37 ng/ml). The average daily water consumption was significantly lower in the MR group (MR, 29.71 ± 0.86 vs NR, 34.14 ± 1.06 L head -1 day-1), while there was a tendency for the roughage intake to be higher in the MR group compared to that of the NR group (MR, 5.88 ± 0.18 vs NR, 6.44 ± 0.19 kg head-1 -1 day-1; P = 0.0508). It was concluded that roof modification facilitated a reduction in heat load from roof re-radiation, and was an effective means of alleviating thermal stress in young buffaloes.

Khongdee, Titaporn; Sripoon, S.; Vajrabukka, C.

2013-05-01

105

Intramuscular kinetics and dosage regimens for pralidoxime in buffalo calves (Bubalus bubalis).  

PubMed

The plasma levels, disposition kinetics and a dosage regimen for pralidoxime (2-PAM) were investigated in male buffalo calves following single intramuscular administration (15 or 30 mg/kg). The effects of 2-PAM on various blood enzymes were also determined. The absorption half-life, elimination half-life, apparent volume of distribution and total body clearance of 2-PAM were 1.08 +/- 0.19 h, 3.14-3.19 h, 0.83-1.01 L/kg and 184.9-252.1 ml/(kg h), respectively. At doses of 15 and 30 mg/kg body weight, a plasma concentration > or = 4 microg/ml was maintained for up to 4 and 6 h, respectively. Pralidoxime significantly lowered the serum level of transferases, phosphatases and lactate dehydrogenase but did not influence the acetylcholinesterase and carboxylesterase enzymes. The most appropriate dosage regimen for 2-PAM in the treatment of organophosphate toxicity in buffaloes would be 25 mg/kg followed by 22 mg/kg at 8 h intervals. PMID:11432431

Srivastava, A K; Malik, J K

2001-05-01

106

Ovarian follicular dynamics in water buffalo  

Microsoft Academic Search

The pattern of growth and regression of ovarian follicles was characterized during 7 complete estrous cycles in 5 water buffalo by daily ultrasonographic examinations of the ovaries. Follicles ? 4 mm were measured and their relative locations within the ovary were determined to follow the sequential development of each individual follicle. Results indicated the presence of either one (n =

M. Taneja; A. Ali; G. Singh

1996-01-01

107

A rapid method for authentication of Buffalo (Bubalus bubalis) meat by Alkaline Lysis method of DNA extraction and species specific polymerase chain reaction.  

PubMed

Buffalo (Bubalus bubalis) meat is a major item of export from India but export of beef i.e. meat from cattle (Bos indicus) is prohibited. Also, adulteration of buffalo meat with that of beef (meat from cattle) is a common fraudulent practice because of prohibition on cow slaughter in most states of India. Food analysts require precise identification techniques to implement such regulations. In the present study, a method of DNA extraction by Alkaline lysis from meat samples and speciation of buffalo meat using species specific Polymerase Chain Reaction (PCR) has been reported. Alkaline lysis technique is a rapid method which involves triturating meat with four volumes of 0.2N NaOH, dilution of resultant liquid extract with eight volumes of 0.2N NaOH, heating the mix 75 °C for 20 min followed by neutralization with eight volumes of 0.04N Tris HCl. Entire procedure of DNA extraction takes less than 30 min and it is economical as it involves less expensive chemicals. Method was successfully applied in animal byproducts also viz., liver, heart and kidney. For authentication of buffalo meat, pair of primers was designed based on mitochondrial D loop gene nucleotide sequence. PCR amplification using the designed primers gave amplicon of size 482 bp in buffalo and no amplification was detected in closely related species viz., cattle, sheep and goat meat samples. Results of the assay were highly repetitive and reliable. An export sample referred by export regulation authorities was also analyzed by using the Alkaline lysis method of DNA extraction and species specific PCR which enabled authentication of meat within 5 h. PMID:24425899

Girish, P S; Haunshi, S; Vaithiyanathan, S; Rajitha, R; Ramakrishna, C

2013-02-01

108

Comparative efficacies of six different media for cryopreservation of immature buffalo (Bubalus bubalis) calf testis.  

PubMed

Buffalo calves have a high mortality rate (~80%) in commercial dairies and testis cryopreservation can provide a feasible option for the preservation of germplasm from immature males that die before attaining sexual maturity. The aim of the present study was to evaluate combinations of 10 or 20% dimethylsulfoxide (DMSO) with 0, 20 or 80% fetal bovine serum (FBS) for cryopreservation of immature buffalo testicular tissues, subjected to uncontrolled slow freezing. Tissues cryopreserved in 20% DMSO with 20% FBS (D20S20) showed total, tubular and interstitial cell viability, number of early apoptotic and DNA-damaged cells, surviving germ and proliferating cells and expression of testicular cell-specific proteins (POU class 5 homeobox (POU5F1), vimentin (VIM) and actin ?2 (ACTA2)) similar to that of fresh cultured control (FCC; P>0.05). Expression of cytochrome P450, family 11, subfamily A (CYP11A1) protein and testosterone assay showed that only tissues cryopreserved in D20S20 had Leydig cells and secretory functions identical to that of FCC (P>0.05). High expression of superoxide dismutase2 (SOD2), cold-inducible RNA-binding protein (CIRBP) and RNA-binding motif protein3 (RBM3) proteins in cryopreserved tissues indicated involvement of cell signalling pathways regulating cellular protective mechanisms. Similarity in expression of pro-apoptosis proteins transcription factor tumour protein P53 (TP53) and BCL2-associated X protein (BAX) in D20S20 cryopreserved tissues to that of FCC (P>0.05) suggested lower apoptosis and DNA damage as key reasons for superior cryopreservation. PMID:25482277

Devi, Lalitha; Makala, Himesh; Pothana, Lavanya; Nirmalkar, Khemlal; Goel, Sandeep

2014-12-01

109

IGF-1 attenuates LPS induced pro-inflammatory cytokines expression in buffalo (Bubalus bubalis) granulosa cells.  

PubMed

Interaction between immune and endocrine system is a diverse process influencing cellular function and homeostasis in animals. Negative energy balance (NEB) during postpartum period in dairy animals usually suppresses these systems resulting in reproductive tract infection and infertility. These negative effects could be due to competition among endocrine and immune signaling pathways for common signaling molecules. The present work studied the effect of IGF-1 (50ng/ml) on LPS (1?g/ml) mediated pro-inflammatory cytokine expression (IL-1?, TNF-?, IL-6) and aromatase (CYP19A1) genes' expressions as well as proliferation of buffalo granulosa cells. The crosstalk between LPS and IGF-1 was also demonstrated through studying the activities of downstream signaling molecules (ERK1/2, Akt, NF-?B) by western blot and immunostaining. Gene expression analysis showed that IGF-1 significantly reduced the LPS induced expression of IL-1?, TNF-? and IL-6. LPS alone inhibited the CYP19A1 expression. However, co-treatment with IGF-1 reversed the inhibitory effect of LPS on CYP19A1 expression. LPS alone did not affect granulosa cell proliferation, but co-treatment with IGF-1, and IGF-1 alone enhanced the proliferation. Western blot results demonstrated that LPS caused the nuclear translocation of the NF-?B and increased the phosphorylation of ERK1/2 and Akt maximum at 15min and 60min, respectively. Nonetheless, co-treatment with IGF-1 delayed LPS induced phosphorylation of ERK1/2 (peak at 120min), while promoting early Akt phosphorylation (peak at 5min) with no effect on NF-?B translocation. Overall, IGF-1 delayed and reversed the effects of LPS, suggesting that high IGF-1 levels may combat infection during critical periods like NEB in postpartum dairy animals. PMID:25433435

Onnureddy K; Ravinder; Onteru, Suneel Kumar; Singh, Dheer

2015-03-01

110

Mensuration of spermatozoa from different levels of the reproductive tract of the buffalo-bull (Bubalus bubalis)  

E-print Network

Mensuration of spermatozoa from different levels of the reproductive tract of the buffalo of the reproductive tract were measured in 6 buffalo-bulls castrated under local anesthesia ; the same measu- rements were taken in the semen of 6 breeding buffalo-bulls. During transit from caput to cauda epididymis

Paris-Sud XI, Université de

111

Neospora caninum serostatus is affected by age and species variables in cohabiting water buffaloes and beef cattle.  

PubMed

The aim of this study was to investigate how Neospora caninum serostatus may be affected by variables such as host species (water buffaloes or cattle) and age in animals cohabiting in the same ranch. A convenience cross-sectional study was performed on four ranches in the Northeast of Argentina, where water buffalo are cohabitating with beef cattle. Blood samples were collected from 1350 female water buffaloes (Bubalus bubalis) and 880 female beef cattle (Bos taurus and Bos indicus crossbreeds) from four ranches. Calving and weaning percentages at herd level for each ranch were also recorded. N. caninum antibody levels were measured by an indirect fluorescent antibody test (IFAT) (reciprocal antibody titers ? 100). Serological results were classified into 2 categories (0: negative; 1: positive). A logistic regression model was used to describe the relationship between N. caninum serostatus and specie (water buffalo or cattle), age or ranch and their interactions. Likelihood ratio tests were used to assess the significance of the model and their terms. Odds ratios were estimated and 95% profile likelihood (LR) and Wald confidence intervals (CI) obtained. Overall, specific antibody titers were found in 43.3% (584/1350) of water buffaloes and 28.6% (252/880) of cattle. Seropositive water buffaloes and cattle were observed on all ranches. Age was statistically significant (p=0.01) with an overall estimate of logit (log odds) of age of 0.03 for both species. This indicates that for every one year increase in age, the expected change in log odds of being seropositive increased by 0.03. On three of four ranches a water buffalo was 4.48, 1.54 and 2.25 times more likely to be seropositive than cattle for animals of the same age. The N. caninum serostatus was affected by age in the first place, but also by species on at least three of the four ranches. Calving and weaning percentages were higher in water buffaloes than in beef cattle (p<0.05). Even though the low pathogenicity that N. caninum seems to have in water buffaloes, this study reinforces the importance of this specie as maintenance of the disease. PMID:24792747

Moore, D P; Konrad, J L; San Martino, S; Reichel, M P; Cano, D B; Méndez, S; Späth, E J L; Odeón, A C; Crudeli, G; Campero, C M

2014-07-14

112

Comparative therapeutic effect of toltrazuril, sulphadimidine and amprolium on Eimeria bovis and Eimeria zuernii given at different times following infection in buffalo calves (Bubalus bubalis).  

PubMed

We compared the therapeutic effect of three anticoccidial drugs (toltrazuril, sulphadimidine and amprolium) in buffalo (Bubalus bubalis) calves experimentally infected with Eimeria bovis (E. bovis) and E. zuernii oocysts (3 x 104oocyst/calf). Buffalo calves (1.5-4 month old, 70-kg body weight) were randomly allocated into 3 groups (9 calves each). Group T was experimentally infected with oocysts and treated with toltrazuril (20 mg/kg BW twice orally at a 1-week interval). Group S was experimentally infected with oocysts and treated with sulphadimidine (125 mg/kg injected IM followed by half dose for 4 successive days). Group A was experimentally infected with oocysts and treated with amprolium (50 mg/kg orally for 7 successive days). Each group had three subgroups (three calves/subgroup) to represent timing of the drug administration: 1st day of coccidia infection (FD), onset of clinical signs of coccidiosis (CC), and onset of oocyst shedding into the faeces (OS). Clinical signs, body-weight gain (BWG) and number of oocysts per gram feces (OPG) were monitored daily for 35 days post-infection (DPI). The OPG were reduced (but the BWG was not different) in the T calves compared to S and A calves. Within the same group, treatment from the 1st day of infection reduced the OPG and increased the BWG compared to the later treatment timings. PMID:18262668

Ghanem, Mohamed M; Radwaan, Mervat E; Moustafa, Abdel Moneim M; Ebeid, Mohamed H

2008-04-17

113

Effects of milk feeding, frequency and concentration on weaning and buffalo (Bubalus bubalis) calf growth, health and behaviour.  

PubMed

Growth, weight at birth and daily weight gain (DWG) on 12 water buffalo calves, starting from 6 days of age until completion of weaning, was investigated in this study. Different feeding regimens were given to two groups of animals with regard to daily milk replacer: (1) group 1 (G1) received a double concentration in single administration; whereas (2) group 2 (G2) received the same amount of milk replacer split twice daily. Blood samples were collected from each calf on days 6, 30, 60 and 90 to evaluate acute phase proteins (haptoglobin), bactericide activity, lysozime, total protein content and biochemical parameters. No differences were observed between the two groups in terms of dry matter intake, feed efficiency and live body weight at the end of the study. Interestingly, a significantly (P?water buffalo calf during weaning. This new approach facilitates calves management, without interfering with calves growing performances. PMID:23712396

Vecchio, Domenico; Di Palo, Rossella; De Carlo, Esterina; Esposito, Luigi; Presicce, Giorgio Antonio; Martucciello, Alessandra; Chiosi, Emilio; Rossi, Pasquale; Neglia, Gianluca; Campanile, Giuseppe

2013-11-01

114

Assessment of genetic variability and structuring of riverine buffalo population (Bubalus bubalis) of Indo-Gangetic basin.  

PubMed

The buffalo population of Uttar Pradesh (UP) constitutes 26.1% of the total buffalo population of India, yet this population has not been classified into distinct breeds or subpopulations due to lack of systematic study. Genetic variation at 30 microsatellite loci was examined and statistical analysis was carried out to reveal genetic diversity, demographic parameters of these buffaloes and to investigate the existence of population substructures underlying geographical distribution. The mean number of alleles per locus was 13.26 and mean effective number of alleles was 3.74, whereas mean observed and expected heterozygosities were found to be 0.57 and 0.67 in UP buffaloes. Principal component analysis (PCA) based on allele frequency data revealed subclustering of UP buffalo population. Bayesian analysis result also revealed clear membership of individuals into five clusters indicating a genetic subdivision within the UP buffalo population. The buffaloes of Western and Central regions of UP were subtly separated while buffaloes of Tarai area and Bhadawari buffaloes revealed distinctive population structure. The buffaloes of Mau, Ballia and Ghazipur districts of Eastern region also had a distinctive genetic structure. The analysis of data on buffaloes of Indo-Gangetic plains revealed that population was in mutation drift equilibrium. The observed mean M ratio in the population was above the critical significance value (Mc) suggesting that it has not suffered any severe reduction in effective population size. The statistical tests revealed a historical constancy of size of buffalo in this geographical area. The high level of genetic variability indicates UP buffalo population is a vast reservoir of genetic diversity and this shall help in taking informed conservation decisions and sustainable utilization. PMID:25380468

Joshi, Jyoti; Salar, R K; Banerjee, Priyanka; Sharma, Upasna; Tantia, M S; Vijh, R K

2015-01-01

115

Bovine herpesvirus type 1 marker vaccine induces cross-protection against bubaline herpesvirus type 1 in water buffalo.  

PubMed

Water buffalo (Bubalus bubalis) are susceptible to bovine herpesvirus type 1 (BoHV-1) and a species-specific herpesvirus, bubaline herpesvirus type 1 (BuHV-1). In this study, an attenuated marker BoHV-1 based vaccine against BuHV-1 challenge was evaluated to determine whether it induces protection from viral replication. One group of water buffalo calves was immunized with an attenuated BoHV-1 marker vaccine. A second group was not vaccinated and used as the control. During the post-vaccination period, we monitored the humoral immune response. The efficacy of the vaccine was tested after intranasal challenge of the calves with a BuHV-1 strain. The experiment showed that after vaccination, BuHV-1 replication was significantly reduced by approximately three titer points compared to the controls. The control animals showed high levels of viral shedding and mild signs associated with BuHV-1 infection. Therefore, our study provides evidence for the existence of cross-protection between BoHV-1 and BuHV-1 in buffalo calves. PMID:24985155

Montagnaro, Serena; De Martinis, Claudio; Iovane, Valentina; Ciarcia, Roberto; Damiano, Sara; Nizza, Sandra; De Martino, Luisa; Iovane, Giuseppe; Pagnini, Ugo

2014-09-01

116

Sarcocystis cafferi, n. sp. (Protozoa: Apicomplexa) from the African buffalo (Syncerus caffer)  

Technology Transfer Automated Retrieval System (TEKTRAN)

Four species of Sarcocystis are currently recognized in the Asian water buffalo (Bubalus bubalis): Sarcocystis fusiformis with macrocysts and cats as definitive hosts, S. buffalonis also with macrocysts and cats as definitive hosts, S. levinei with microcysts and dogs as definitive hosts, and S. dub...

117

Production of interspecies handmade cloned embryos by nuclear transfer of cattle, goat and rat fibroblasts to buffalo ( Bubalus bubalis) oocytes  

Microsoft Academic Search

The possibility of producing interspecies handmade cloned (iHMC) embryos by nuclear transfer from donor cells of cattle, goat and rat using buffalo oocytes as recipient cytoplasts was explored. Zona-free buffalo oocytes were enucleated by protrusion cone-guided bisection with a microblade. After electrofusion with somatic cells, reconstructed oocytes were activated by calcimycin A23187, treated with 6-dimethylaminopurine and were cultured in K-RVCL-50®

N. L. Selokar; A. George; A. P. Saha; R. Sharma; M. Muzaffer; R. A. Shah; P. Palta; M. S. Chauhan; R. S. Manik; S. K. Singla

2011-01-01

118

Hepatic hemosiderosis in buffalo fish ( Ictiobus spp.)  

Microsoft Academic Search

Hepatocellular hemosiderosis was observed in several species of fish associated with mixed chemical pollution in a Mississippi River Basin ecosystem. Three species of buffalo fish—smallmouth buffalo (Ictiobus bubalus), bigmouth buffalo (Ictiobus cyprinellus), and black buffalo (Ictiobus niger)—were collected from a contaminated site, Devil's Swamp, and a control site, Tunica Swamp, both near Baton Rouge, Louisiana. Liver, kidney and spleen were

A. Thiyagarajah; W. R. Hartley; A. Abdelghani

1998-01-01

119

Analysis and functional annotation of expressed sequence tags of water buffalo.  

PubMed

An elucidated genome of domestic livestock river buffalo will contribute enormously to economy and better understanding of genome evolution as well. An attempt is made to obtain genomic information on buffalo, based on total Expressed Sequence Tags (ESTs) of Bubalus bubalis available in public domain. These ESTs were annotated and classified into 15 different functional categories based on their homology to the known proteins. Interestingly, 41.79% of the contigs were found to be buffalo specific novel ESTs with respect to other species used in analysis which needs further studies. Also, 224 pSNPs (putative Single Nucleotide Polymorphism) were detected. This study will provide a home base for further genomic studies of buffalo and comparative studies enabling a starting point for the genome annotation of the organism. Supplementary materials are available for this article online. PMID:23394367

Bajetha, Garima; Bhati, Jyotika; Sarika; Iquebal, M A; Rai, Anil; Arora, Vasu; Kumar, Dinesh

2013-01-01

120

Body condition, energy balance and immune status of periparturient Murrah buffaloes (Bubalus bubalis) supplemented with inorganic chromium.  

PubMed

Periparturient Murrah buffaloes were used to determine whether body condition, energy balance and immune status are affected by inorganic Cr supplementation. Twenty-four Murrah buffaloes were blocked into four groups having six animals in each group and fed for 60 days pre-partum to 150 days post-partum. Feeding regimen was same in all the groups except that these were supplemented with 0.0, 0.5, 1.0 and 1.5 of Cr per kilogram of dry matter (DM) in the four respective groups. Buffaloes were weighed at fortnightly intervals. Blood samples were collected from the jugular vein at days -60, -30, -15, -7, 0, 7, 15, 30, 60, 90, 120 and 150 of experimental feeding for the estimation of non-esterified fatty acid (NEFA), beta-hydroxybutyric acid (BHBA), Cr level, lymphocyte proliferation, neutrophil phagocytic activity, plasma total immunoglobulin (TIg), immunoglobulin G (IgG) and cortisol levels. Results revealed that with approaching parturition, dry matter intake (DMI), immune response and plasma Cr level decreased (P < 0.05) gradually and minimum values were observed on the day of parturition in all groups. In contrast, body condition score (BCS), plasma NEFA and BHBA concentrations showed increasing (P < 0.05) trends towards calving and level decreased after calving. Dietary Cr supplementation did not have any effect on DMI and BCS, but immune response and plasma Cr concentration showed a positive correlation with dietary Cr supplementation. Buffaloes supplemented with 1.5 mg/kg Cr had significantly (P < 0.05) low plasma NEFA and BHBA concentrations. The results of present findings indicated that dietary inorganic Cr supplementation reduced lipid mobilization and improved immune response in periparturient buffaloes. PMID:25037066

Deka, Rijusmita Sarma; Mani, Veena; Kumar, Muneendra; Shiwajirao, Zade Satish; Tyagi, Amrish Kumar; Kaur, Harjit

2014-10-01

121

Clinical outcomes and molecular genotyping of Staphylococcus aureus isolated from milk samples of dairy primiparous Mediterranean buffaloes (Bubalus bubalis).  

PubMed

Staphylococcus aureus is one of the most important pathogens causing mastitis in dairy cows and in Mediterranean buffaloes. Genotype B (GTB) is contagious in dairy cows and may occur in up to 87% of cows of a dairy herd. It was the aim of this study to evaluate genotypes present, clinical outcomes, and prevalence of Staph. aureus in milk samples of primiparous Mediterranean dairy buffaloes. Two hundred composite milk samples originating from 40 primiparous buffaloes were collected from May to June 2012, at d 10, 30, 60, 90, and 150 d in milk (DIM) to perform somatic cell counts and bacteriological cultures. Daily milk yields were recorded. Before parturition until 40 to 50 DIM, all primiparous animals were housed separated from the pluriparous animals. Milking was performed in the same milking parlor, but the primiparous animals were milked first. After 50 DIM, the primiparous were mixed with the pluriparous animals, including the milking procedure. Individual quarter samples were collected from each animal, and aliquots of 1 mL were mixed and used for molecular identification and genotyping of Staph. aureus. The identification of Staph. aureus was performed verifying the presence of nuc gene by nuc gene PCR. All the nuc-positive isolates were subjected to genotype analysis by means of PCR amplification of the 16S-23S rRNA intergenic spacer region and analyzed by a miniaturized electrophoresis system. Of all 200 composite samples, 41 (20.5%) were positive for Staph. aureus, and no genotype other than GTB was identified. The prevalence of samples positive for Staph. aureus was 0% at 10 DIM and increased to a maximum of 22/40 (55%) at 90 DIM. During the period of interest, 14 buffaloes tested positive for Staph. aureus once, 6 were positive twice, and 5 were positive 3 times, whereas 15 animals were negative at every sampling. At 90 and 150 DIM, 7 (17.5%) and 3 buffaloes (7.5%), respectively, showed clinical mastitis (CM), and only 1 (2.5%) showed CM at both samplings. At 60, 90, and 150 DIM, 1 buffalo was found with subclinical mastitis at each sampling. At 30, 60, 90, and 150 DIM, 2.5 (1/40), 22.5 (9/40), 35 (14/40), and 10% (4/40) were considered affected by intramammary infection, respectively. Buffaloes with CM caused by Staph. aureus had statistically significantly higher mean somatic cell count values (6.06 ± 0.29, Log10 cells/mL ± standard deviation) and statistically significantly lower mean daily milk yields (7.15 ± 1.49, liters/animal per day) than healthy animals (4.69 ± 0.23 and 13.87 ± 2.64, respectively), buffaloes with IMI (4.82 ± 0.23 and 11.16 ± 1.80, respectively), or with subclinical mastitis (5.47 ± 0.10 and 10.33 ± 0.68, respectively). Based on our knowledge, this is the first time that Staph. aureus GTB has been identified in milk samples of dairy Mediterranean buffaloes. PMID:25459906

Guccione, J; Cosandey, A; Pesce, A; Di Loria, A; Pascale, M; Piantedosi, D; Steiner, A; Graber, H U; Ciaramella, P

2014-12-01

122

Phylogeography and domestication of Indian river buffalo  

PubMed Central

Background The water buffalo- Bubalus bubalis holds tremendous potential in livestock sector in many Asian countries, particularly India. The origin, domestication and genetic structure of the Indian river buffalo are poorly understood. Therefore, to understand the relationship among the maternal lineages of Indian river buffalo breeds and their domestication process, we analysed mitochondrial D-loop region of 217 animals representing eight breeds from eight different locations in India along with published sequences of Mediterranean buffalo. Results The maximum parsimony tree showed one major clade with six internal branches. Reduced median network revealed expansion from more than one set of haplotypes indicating complex domestication events for this species. In addition, we found several singleton haplotypes. Using rho statistics, we obtained a time estimate of 6300 years BP for the expansion of one set of hapltoypes of the Indian domestic buffalo. A few breed specific branches in the network indicated an ancient time depth of differentiation of some of the maternal lineages of river buffalo breeds. The multidimensional display of breed pairwise FST values showed significant breed differentiation. Conclusion Present day river buffalo is the result of complex domestication processes involving more than one maternal lineage and a significant maternal gene flow from the wild populations after the initial domestication events. Our data are consistent with the available archaeological information in supporting the proposition that the river buffalo was likely to be domesticated in the Western region of the Indian subcontinent, specifically the present day breeding tracts of the Mehsana, Surati and Pandharpuri breeds. PMID:17915036

Kumar, Satish; Nagarajan, Muniyandi; Sandhu, Jasmeet S; Kumar, Niraj; Behl, Vandana

2007-01-01

123

Repeated ultrasound-guided transvaginal oocyte retrieval from cyclic Murrah buffaloes (Bubalus bubalis): oocyte recovery and quality.  

PubMed

The present study was undertaken to explore the potential of the Murrah breed of buffaloes as donors of oocytes and to find out the recovery rate and oocyte quality in cyclic Murrah buffaloes subjected to oocyte recovery once a week. Murrah buffaloes (n = 5) were synchronized for estrus by a single prostaglandin injection schedule. The animals were subjected to transvaginal oocyte retrieval (TVOR) once weekly for 6 weeks, starting from Day 7 of the oestrous cycle (Day 0 = day of oestrus). TVOR was performed using an ultrasound machine with a 5 MHz transvaginal transducer, single lumen 19-gauge, 60 cm long needle and a constant vacuum pressure of 50 mmHg. The number and size of follicles in each ovary was determined before puncture. The follicles were characterized on the basis of their diameter as small (3-5 mm), medium (6-9 mm) and large (> or = 10 mm). The oocytes recovered were classified as grade A, cumulus-oocytes complexes (COCs) with > or = 5 layers of cumulus cells; grade B, those with two to four layers; grade C, partially denuded oocytes; and grade D, completely denuded oocytes. The mean (+/- S.E.M) number of small, medium and large follicles, and the number of total follicles observed per animal per session, which was 2.2 +/- 0.3, 0.6 +/- 0.2, 0.9 +/- 0.1 and 3.7 +/- 0.3, respectively, did not differ between animals or between puncture sessions. Small follicles constituted a major proportion (59%) of the total observed follicles. A mean (+/- S.E.M) number of 3.0 +/- 0.3 follicles were punctured and 2.0 +/- 0.3 oocytes recovered per animal per session, with a recovery rate of 68%. Out of the total 61 oocytes recovered, 36 (59%) were of grades A + B whereas 25 (41%) were of grades C + D. In conclusion, this study describes the potential of cyclic Murrah buffaloes as donors of oocytes collected by repeated TVOR once a week, without any adverse effects on follicular growth and oocyte recovery. It also describes an efficient system for carrying out TVOR in buffaloes. PMID:15913926

Gupta, V; Manik, R S; Chauhan, M S; Singla, S K; Akshey, Y S; Palta, P

2006-01-01

124

Cellular coupling of potassium channels with beta2 adrenoceptors in mediating myometrial relaxation in buffaloes (Bubalus bubalis).  

PubMed

Present study unravels the functional presence of potassium channels and their involvement in mediating beta(2) adrenoceptors-induced myometrial relaxation in buffalo myometrium. Isolated myometrial preparations exhibited rhythmic spontaneity with regular pattern of amplitude and frequency. Levcromakalim produced concentration-dependent inhibitory effect on myometrial spontaneity and relaxant effect and the dose-response curve (DRC) was shifted towards right in the presence of glybenclamaide. In the tissues pretreated with glybenclamide, relaxant effect of albuterol was significantly (P < 0.05-0.001) lower (pD(2) = 6.94, R(max) = 96.8 +/- 3.3%; n = 5) compared with albuterol alone (pD(2) = 8.55, R(max) = 101.1 +/- 6.3%; n = 6) and the DRC was shifted to right. In the presence of tetraethyl ammonium (TEA) also, significant (P < 0.001) rightward shift of DRC of albuterol with decrease in maximal effect (pD(2) = 8.05, R(max) = 71.2 +/- 7.4%; n = 7 vs. control pD(2) = 8.55, R(max) = 101.1 +/- 6.3%; n = 6) was observed. On the other hand, 4-aminopyridine (1 mm) sensitized the myometrial strips and increased the amplitude and frequency/min of myometrial spontaneity but failed to significantly alter the DRC of albuterol. Results of present study suggest the functional presence of K(ATP), BK(Ca) and K(V) channels in buffalo myometrium, but beta(2)-adrenoreceptor agonist-induced myometrial relaxation seems to be K(ATP) and BK(Ca) channel-dependent only. Further, our studies also suggest promising therapeutic potential of potassium channel modulators as tocolytics in buffaloes. PMID:20444021

Choudhury, S; Garg, S K; Singh, T U; Mishra, S K

2010-02-01

125

Effect of Additional Chromium Supplementation on Health Status, Metabolic Responses, and Performance Traits in Periparturient Murrah Buffaloes (Bubalus bubalis).  

PubMed

This study was conducted to determine the effects of inorganic chromium (Cr) on body condition, metabolic responses, lactation performance, and reproductive parameters in periparturient Murrah buffaloes. Twenty-four multiparous advanced pregnant Murrah buffaloes were randomly assigned to four treatment diets. Feeding regimen was the same in all the groups, except that the animals in the four respective groups were additionally supplemented with 0.0, 0.5, 1.0, and 1.5 mg of Cr/kg dry matter (DM) from day 60 prepartum to 150 days postpartum. Dry matter intake (DMI) and milk production were recorded every day, while body condition score (BCS) and whole blood samples were collected at days -60, -45, -30, -15, -7, -3, 0, 3, 7, 15, 30, 45, 60, 90, 120, and 150 relative to actual calving. As the days to calving advanced, DMI, plasma glucose, insulin, leptin, and Cr levels decreased (P?buffaloes. These results could be interpreted as an improvement in the body condition, metabolic response, milk yield, efficiency of milk production and nutrient utilization, and reproductive performance of peripartum Murrah buffaloes. PMID:25388756

Deka, Rijusmita Sarma; Mani, Veena; Kumar, Muneendra; Zade, Shiwajirao Satish; Upadhaya, Ramesh Chand; Kaur, Harjit

2015-02-01

126

Multilocus phylogeographical analysis of Trypanosoma (Megatrypanum) genotypes from sympatric cattle and water buffalo populations supports  

E-print Network

and water buffalo populations supports evolutionary host constraint and close phylogenetic relationships in Brazil found at least four genotypes infecting cattle (Bos taurus), but only one in water buffalo from water buffalo and 28 from cattle from four separate loca- tions in Brazil and Venezuela. Multigene

Tyler, Charles

127

Equivalency of Buffalo (Bubalus Bubalis) Embryonic Stem Cells Derived From Fertilized, Parthenogenetic, and Hand-Made Cloned Embryos  

PubMed Central

Abstract This study was aimed at establishing buffalo embryonic stem cells (ESCs) from in vitro fertilized (IVF), parthenogenetic, and hand-made cloned (HMC) embryos and to check their equivalency in terms of stem cell marker expression, longevity, proliferation, and differentiation pattern. ESCs derived from all three sources were found by immunofluorescence to express the pluripotency markers SSEA-4, TRA-1-60, TRA-1-81, OCT4, and SOX2 and were able to form embryoid bodies containing cells expressing genes specific to endoderm (AFP, HNF4, and GATA4), mesoderm (MSX1, BMP4, and ASA), and ectoderm (cytokeratin 8 and NF68). Reverse transcriptase PCR (RT-PCR) showed cells from all sources to be positive for pluripotency markers OCT4, SOX2, NANOG, STAT3, REX1, FOXD3, NUCLEOSTEMIN, and TELOMERASE. Pluripotency markers OCT4, SOX2, NANOG, and c-MYC were also analyzed by real-time PCR. No significant differences were observed among ESCs from all three sources for all these genes except NANOG, whose expression was higher (p<0.05) in HMC-derived ESCs (6.897±2.3) compared to that in parthenogenesis- and IVF-derived cells (1.603±0.315 and 1±0, respectively). Pluripotent, stable buffalo ESC lines derived from IVF, parthenogenesis, and HMC embryos may be genetically manipulated to provide a powerful tool for studies involving embryonic development, genomic imprinting, gene targeting, cloning, chimera formation, and transgenic animal production. PMID:22582863

Muzaffar, Musharifa; Selokar, Naresh L.; Singh, Karn P.; Zandi, Mohammad; Singh, Manoj K.; Shah, Riaz A.; Chauhan, Manmohan S.; Singla, Suresh K.; Palta, Prabhat

2012-01-01

128

HAEMODYNAMIC ALTERATIONS DURING ELECTROANAESTHESIA IN YOUNG BUFFALOES.  

E-print Network

HAEMODYNAMIC ALTERATIONS DURING ELECTROANAESTHESIA IN YOUNG BUFFALOES. COMPARATIVE STUDY in buffaloes (Bubalus bubalis) to determine which wave form of current will produce the most effective anaes buffalo calves, one to two years of age, were the subjects of the present inves- tigation. All the calves

Paris-Sud XI, Université de

129

135 effect of L-ergothioneine supplementation during culture on in vitro embryo development in buffalo (bubalus bubalis).  

PubMed

It is known that in vitro mammalian embryo development is negatively affected by the increased oxidative stress occurring under culture conditions. The oxidative damage of cell components via reactive oxygen species interferes with proper cell function. Buffalo embryos are particularly sensitive to oxidative stress because of their high lipid content (Boni et al. 1992 Acta Med. Vet. 38, 153-161). l-Ergothioneine (LE) is a powerful scavenger of hydroxyl radicals (OH) and an inhibitor of iron or copper ion-dependent generation of OH from hydrogen peroxide (H2O2). The aim of this study was to evaluate whether enriching the in vitro-culture medium with LE improves in vitro embryo production efficiency in buffalo. Abattoir-derived buffalo oocytes (n=854, over 6 replicates) were in vitro matured and fertilized according to standard procedures (Gasparrini et al. 2006 Theriogenology 65, 275-287). Twenty hours after IVF presumptive zygotes were cultured in SOFaa supplemented by 8mgmL(-1) BSA in a controlled gas atmosphere consisting of 5% CO2, 7% O2, 88% N2, in humidified air, at 38.5°C with 0 (control; n=214), 0.05mM LE (n=217), 0.1mM LE (n=204), and 1mM LE (n=219). Cleavage rate was assessed at the time of change of culture (Day 5) and the cleaved elements were cultured for a further 2 days. The embryos obtained by the end of culture, i.e. on Day 7 post-IVF, were scored for quality, based on morphological criteria, and for developmental stage, as previously described (Robertson, Nelson 2010 Manual of the International Embryo Transfer Society 86-105). The percentages of total transferable embryos and Grade 1 and 2 blastocysts in relation to cleaved oocytes were recorded. Because the chronology of development is known to be one of the most reliable parameters for assessing quality, the percentage of fast-developing embryos, i.e. hatched and expanded blastocysts, was also recorded. Data were analysed by Chi-squared test. Cleavage rate was not affected by the treatment (71.4, 66.8, 68.7, and 63.0%, respectively, with 0, 0.05, 0.1, and 1mM LE). The total embryo output increased in groups supplemented with 0.05 and 0.1mM LE (31.3, 42.2, 43.8, and 21.7%, respectively, with 0, 0.05, 0.1, and 1mM LE; P<0.05). However, the enrichment of in vitro culture with 0.1mM LE also increased the percentage of Grade 1 and 2 blastocysts compared with the control and to 1mM LE (21.6, 30.9, 33.9, and 21.7%, respectively, with 0, 0.05, 0.1, and 1mM LE; P<0.05). Likewise, 0.1mM LE gave higher percentages of fast developing embryos than the control and 1mM LE groups. In conclusion, these results demonstrated a beneficial effect of LE during culture on buffalo in vitro embryo development. The dose response trial indicated that the optimal concentration is 0.1mM that also influenced the chronology of development and hence embryo viability. PMID:25472184

Zullo, G; Salzano, A; Bifulco, G; Longobardi, V; Albero, G; Neglia, G; Gasparrini, B

2014-12-01

130

Ion, protein, phospholipid and energy substrate content of oviduct fluid during the oestrous cycle of buffalo (Bubalus bubalis).  

PubMed

The aim of this research was to analyse the composition of oviduct fluid (ODF) in buffalo cows at different oestrous cycle phases to fulfil the requirements of buffalo embryos in vitro. ODF was collected by chronic cannulation from three cows that were synchronized by administering a synthetic prostaglandin. Based on hormonal profiles, the pre-ovulatory, ovulatory, post-ovulatory and luteal phases of the oestrous cycle were defined. The volume of ODF produced (ml/24 h) was influenced by the oestrous cycle, with values (mean ± SE) around ovulation (1.0 ± 0.2) greater (p < 0.05) than in both the luteal (0.4 ± 0.1) and the post-ovulatory phases (0.5 ± 0.1), but not different from the intermediate values in the pre-ovulatory phase (0.8 ± 0.2). Among cycle phases, no differences were found in sodium, potassium, calcium and magnesium concentrations (130.0 ± 1.1, 5.1 ± 0.3, 2.8 ± 0.1 and 0.59 ± 0.04 mmol/l respectively). Interestingly, the chloride secretion (?m/24 h) was higher (p < 0.05) at ovulation (150.2 ± 16.5) than during both the luteal (73.7 ± 22.0) and the post-ovulatory phases (63.7 ± 11.2), with intermediate values in the pre-ovulatory phase (113.4 ± 23.5). Glucose concentration (mmol/l) was higher (p = 0.056) in the pre-ovulatory phase (0.06 ± 0.02) than in the luteal (0.02 ± 0.01) and post-ovulatory (0.02 ± 0.01) phases but not different from values in the ovulatory phase (0.04 ± 0.02). Concentrations of pyruvate and lactate among oestrous cycle phases were similar (0.08 ± 0.01 and 1.0 ± 0.1 mmol/l respectively). The total quantity of phospholipids (?mol/24 h) was greater (p < 0.05) at ovulation (0.21 ± 0.02) compared with the luteal, pre-ovulatory and post-ovulatory phases of the cycle (0.09 ± 0.02, 0.13 ± 0.02 and 0.09 ± 0.01 respectively). No differences were found in either the protein concentration (1.8 ± 0.3 mg/ml) or the quantity of proteins secreted in 24 h (1.8 ± 0.4 mg) among oestrous cycle phases. In conclusion, this study provides the first characterization of buffalo ODF during the oestrous cycle, showing species-specific differences that may be useful for developing suitable media for buffalo in vitro embryo production. PMID:19761531

Vecchio, D; Neglia, G; Di Palo, R; Campanile, G; Balestrieri, M L; Giovane, A; Killian, G; Zicarelli, L; Gasparrini, B

2010-10-01

131

First report of isolation and molecular characterization of bubaline herpesvirus 1 (BuHV1) from Argentinean water buffaloes.  

PubMed

Herpesviruses have mainly co-evolved with their hosts for millions of years. However, bovine herpesvirus 1 (BoHV1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. Bubaline herpesvirus 1 (BuHV1) is an alphaherpesvirus closely related to BoHV1 and BoHV5. According to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of BoHV1-related virus infection in wild and domestic ruminant species. Recent studies in Argentina showed an increase in serological prevalence against BoHV1 related viruses in water buffaloes (Bubalus bubalis) population. The aim of this study was to investigate the presence of related ruminant alphaherpesvirus in the Argentinean water buffalo population. BuHV1 was successfully isolated from 5 out of 225 buffaloes analyzed. One isolate was obtained from nasal secretions, and the others were from vaginal swabs. The buffaloes belonged to four different farms located in northeastern Argentina. The isolates were characterized as alphaherpesvirus by direct immunofluorescence using FITC-anti-BoHV1 IgG. Restriction analysis performed with BamHI and BstEII on the complete genome showed differences between the isolates and those from BoHV1 and BoHV5 subtypes. Phylogenetic analysis on both UL27 and US6 showed similarity in tree topology. While three of the isolates grouped together with sequences of BoHV5, two other isolates clustered separately. Genetic analysis of eight concatenated sequences from all isolates and references strains showed high nucleotide sequence identity between BuHV1 and BoHV5. While three of the isolates clustered together with the BoHV5 reference strain, the last two isolates were closely related to an Australian BuHV1 strain. To our knowledge, this is the first report on the isolation and molecular characterization of BuHV1 in South America. Phylogenetic analysis suggested that two different BuHV1 lineages circulate in the Argentinean water buffalo population. PMID:24938487

Maidana, Silvina S; Konrad, José L; Craig, María I; Zabal, Osvaldo; Mauroy, Axel; Thiry, Etienne; Crudeli, Gustavo; Romera, Sonia A

2014-11-01

132

Efficiency to reach age of puberty and behaviour of buffalo heifers (Bubalus bubalis) kept on pasture or in confinement.  

PubMed

In order to evaluate the influence of rearing system (free-ranging (FR) v. confinement (C)) on buffalo heifer efficiency to reach age of puberty and on behavioural and immune functions, two experiments were conducted from September 2010 to October 2011. In Experiment I, 32 subjects aged 8 to 9 months at the start of experiment were used. A total of 16 animals (group C) were group housed in an indoor slatted floor pen (4 m2/animal) with an outdoor paddock (4 m2/animal); 16 others grazed on a Mediterranean natural pasture of 40 ha (group FR). Behavioural data were collected and organic matter digestibility, blood metabolites and progesterone were determined. At the end of the experiment, a novel object test and a skin test were conducted, and the avoidance distance (AD) at the manger was measured. Free-ranging animals were able to express natural behaviours such as wallowing and grazing. C animals devoted more time to the novel object than FR animals, whereas AD at manger was lower in group FR than in group C (P<0.01). Cellular immune response was higher in FR heifers than in C animals (P<0.01). FR animals also showed a higher digestibility of organic matter (P<0.01). Heifers from group FR had higher plasma concentrations of non-esterified fatty acids (P<0.001) and lower concentrations of glucose than heifers from group C (P<0.001). C animals showed higher daily weight gains (P<0.01) and weight at the puberty (P<0.05), but there were no differences in terms of age of puberty between the two groups. The intakes of dry matter (DM), CP and energy to reach the age of puberty were similar in both groups. In order to verify whether the results obtained in Experiment I could be replicated in different rearing conditions (reduced pasture availability, different location and altitude), a second experiment was conducted on 26 animals, where only onset of age of puberty and metabolic profile were monitored. In Experiment II, 13 heifers grazed on a natural pasture of 5 ha, other 13 received the same space as indicated for Experiment I. Results from Experiment II generally confirmed those of Experiment I. Only the intakes of DM and energy to reach the age of puberty were higher in group C than in FR (P<0.001). A lower competition with human nutrition, reproductive performances similar to those shown by confined animals and the indications given by immune and behavioural variables, suggest that a free-range-based system may be conveniently used for buffalo heifer farming purposes. PMID:25076110

Sabia, E; Napolitano, F; De Rosa, G; Terzano, G M; Barile, V L; Braghieri, A; Pacelli, C

2014-11-01

133

Faecal chemical cues in water buffalo that facilitate estrus detection.  

PubMed

Chemo-signals are among the reliable non-invasive methods for estrus detection in mammals. Water buffalo is a silent heat animal and, hence, there is search for chemo-signals which would be effective non-invasive indicators of estrus state. We analyzed the faecal chemical cues during the estrous cycle in buffalo and to find the estrus-specific faecal volatile compounds adopting bull behavior assay. The faecal samples were collected at three phases of the estrous cycle (i.e., proestrus, estrus and postestrus) and subjected to gas chromatography-mass spectrometry analyses. We found 27 volatile compounds in the faeces of buffaloes, of which 4-methyl phenol (4mp) and trans-verbenol (tv) were found only in estrus faeces. The faecal samples of estrus buffaloes and the estrus-specific compound(s) (4mp+tv) at three different concentrations were tested for behavioral responses (flehmen and mounting behavior) in the bull. The bulls exhibited repeated flehmen when exposed to a combination of the two compounds (i.e., 4mp+tv) as compared to the individual compounds or raw faecal sample collected from buffalo when in estrus (P<0.05). However, higher number of mounting behavior was recorded when bulls were exposed to 4mp followed by a combination of the two compounds (4mp+tv) and trans-verbenol (P<0.05), in that order. By contrast, less number of mounting behavior was exhibited by bulls when exposed to the control sample (i.e., Hexadecanoic acid) (P<0.05). As inferred from the bull behavior assay, the present study suggests that the two compounds, 4 methyl phenol and trans-verbenol would be reliable indicators of estrus in buffaloes. PMID:23570909

Karthikeyan, Kandasamy; Muniasamy, Samuthirapandi; SankarGanesh, Devaraj; Achiraman, Shanmugam; Ramesh Saravanakumar, Veluchamy; Archunan, Govindaraju

2013-05-01

134

PCR detection of Neospora caninum in water buffalo foetal tissues.  

PubMed

The seroprevalence of Neospora caninum was surveyed by an ELISA kit on two water buffalo herds of Southern Italy. Seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. Tissue samples collected from three aborted fetuses from the same herds were investigated for N. caninum presence by PCR assays targeting the 18S and the Nc5 DNA sequences, respectively. Both methods have shown the presence of N. caninum DNA in heart and brain. Sequencing of the Nc5 genomic DNA confirmed the presence of N. caninum in the samples; phylogenetic analysis of the obtained sequences showed high homology among the Neospora recovered from different samples. The present study suggests an important role of N. caninum as a possible abortive agent for water buffaloes. PMID:24570043

Auriemma, Clementina; Lucibelli, Maria Gabriella; Borriello, Giorgia; De Carlo, Esterina; Martucciello, Alessandra; Schiavo, Lorena; Gallo, Amalia; Bove, Francesca; Corrado, Federica; Girardi, Santa; Amoroso, Maria Grazia; ?egli Uberti, Barbara; Galiero, Giorgio

2014-03-01

135

Polymorphism of mitochondrial DNA (mtDNA) in cattle and buffaloes  

Microsoft Academic Search

Mitochondrial DNA (mtDNA) from two breeds of cattle, viz., [Hariana (Bos indicus), Holstein (Bos taurus)] and Indian water buffalo (Bubalis bubalus), was analyzed using 13 restriction endonucleases which recognized an average of about 40 six-base sites. Polymorphism among\\u000a cattle was detected with six of these enzymes. The two Holstein differed at six sites, whereas the Hariana breed (Bos indicus) did

P. P. Bhat; B. P. Mishra; P. N. Bhat

1990-01-01

136

Malignant Catarrhal Fever: An Emerging Disease in the African Buffalo (Syncerus caffer).  

PubMed

Within the tribe Bovini in the subfamily Bovinae, the water buffalo (Bubalus Bubalis), American bison (Bison bison), European bison (Bubalus bonasus) and yak (Bos grunniens) are recognized as species highly susceptible to malignant catarrhal fever (MCF). In contrast, the lack of reports describing clinical MCF in the African buffalo (Syncerus caffer) whether free ranging or captive has led to a perception that African buffaloes are resistant to MCF. During the last decade, several cases of MCF in African buffaloes were confirmed in South Africa and experience with seven of these cases is described in this report. Detection of viral nucleic acid in blood or tissues was successful in six African buffaloes that suffered from clinical signs compatible with MCF. Four were positive for infection with ovine herpesvirus type 2 (the causative virus of sheep-associated MCF), and two were positive for alcelaphine herpesvirus type 1 (causative virus of wildebeest-associated MCF). Histopathological examination of tissue samples from all the animals yielded typical lesions that were consistent with those described for MCF in domestic cattle. Developments in the management of African buffaloes translocated from their traditional habitats have likely contributed to the identification of another susceptible host in the subfamily Bovinae. PMID:23957274

Pfitzer, S; Last, R; Espie, I; van Vuuren, M

2013-08-20

137

Bacterial diversity associated with feeding dry forage at different dietary concentrations in the rumen contents of Mehshana buffalo (Bubalus bubalis) using 16S pyrotags.  

PubMed

Pyrosequencing of 16S rRNA gene targeting bacteria was applied to identify diet-induced shifts in the microbiome of both solid and liquid ruminal fractions retrieved from water buffalo fed different diets. The depth of coverage of metabolically active bacteria in a community using different primer pairs was also investigated. To assess reproducibility, animal to animal variation was considered in all phylogenetic and community comparisons. The experiment included four non-lactating water buffaloes fed three different diets for six weeks each; diets were M1 (50% concentrate: 50% dry roughage), M2 (25% concentrate: 75% dry roughage) and M3 (100% dry roughage). A total of 333, 851 pyrotags were analyzed in this study. Phylogenetic analysis revealed significant differences in the rumen microbiome mediated by primer and diet (P < 0.05). Differences in community composition due to primer, diet, fraction and animal were compared using unweighted and weighted UniFrac analysis. Clustering of communities was largely explained by primer differences in both weighted and unweighted UniFrac analyses (P < 0.001). In the weighted analysis, communities clustered by diets (P < 0.05) and fractions (P < 0.08) while no inter-animal variation was observed. The identified repertoire of bacterial populations was dependent on the primer pair, as targeting the V4-V5 region resulted in greater diversity profiles of the microbiome. Within each primer pair, dietary changes altered the community composition with noticeable shifts at genus level. Genera such as Ruminococcus and Fibrobacter (P < 0.05) were higher in abundance on M3 diet while Prevotella dominated (P < 0.05) on M1 diet. PMID:24315806

Pitta, D W; Kumar, S; Veiccharelli, B; Parmar, N; Reddy, B; Joshi, C G

2014-02-01

138

VOL. 31, No.4 UNL WATER CENTERIENVIRONMENTAL PROGRAMS AUGUST 1999 From Buffalo to Bottled Water,  

E-print Network

VOL. 31, No.4 UNL WATER CENTERIENVIRONMENTAL PROGRAMS AUGUST 1999 Current From Buffalo to Bottled Water, Summer Water Tour Attracts Newcomers by Steve Ress The diversity of northeast and north central ebraska's agriculture and water resources attracted 80 people, many of them first-time participants

Nebraska-Lincoln, University of

139

Genetic diversity in Trypanosoma theileri from Sri Lankan cattle and water buffaloes.  

PubMed

Trypanosoma theileri is a hemoprotozoan parasite that infects various ruminant species. We investigated the epidemiology of this parasite among cattle and water buffalo populations bred in Sri Lanka, using a diagnostic PCR assay based on the cathepsin L-like protein (CATL) gene. Blood DNA samples sourced from cattle (n=316) and water buffaloes (n=320) bred in different geographical areas of Sri Lanka were PCR screened for T. theileri. Parasite DNA was detected in cattle and water buffaloes alike in all the sampling locations. The overall T. theileri-positive rate was higher in water buffaloes (15.9%) than in cattle (7.6%). Subsequently, PCR amplicons were sequenced and the partial CATL sequences were phylogenetically analyzed. The identity values for the CATL gene were 89.6-99.7% among the cattle-derived sequences, compared with values of 90.7-100% for the buffalo-derived sequences. However, the cattle-derived sequences shared 88.2-100% identity values with those from buffaloes. In the phylogenetic tree, the Sri Lankan CATL gene sequences fell into two major clades (TthI and TthII), both of which contain CATL sequences from several other countries. Although most of the CATL sequences from Sri Lankan cattle and buffaloes clustered independently, two buffalo-derived sequences were observed to be closely related to those of the Sri Lankan cattle. Furthermore, a Sri Lankan buffalo sequence clustered with CATL gene sequences from Brazilian buffalo and Thai cattle. In addition to reporting the first PCR-based survey of T. theileri among Sri Lankan-bred cattle and water buffaloes, the present study found that some of the CATL gene fragments sourced from water buffaloes shared similarity with those determined from cattle in this country. PMID:25554063

Yokoyama, Naoaki; Sivakumar, Thillaiampalam; Fukushi, Shintaro; Tattiyapong, Muncharee; Tuvshintulga, Bumduuren; Kothalawala, Hemal; Silva, Seekkuge Susil Priyantha; Igarashi, Ikuo; Inoue, Noboru

2015-01-30

140

Gill histopathology of two species of buffalo fish from a contaminated Swamp  

Microsoft Academic Search

Two species of buffalo fish, smallmouth buffalo (Ictiobus bubalus) and bigmouth buffalo (Ictiobus cyprinellus), were collected from a contaminated (multiple metals and organic chemicals) Mississippi River Basin ecosystem, Devil's Swamp and a control site, Tunica Swamp, both near Baton Rouge, Louisiana, USA. The buffalo fish were examined for general health and histopathological effects. This research consists of an analysis of

Arunthavarani Thiyagarajah; William R. Hartley; Sharee E. Major; Michael W. Broxson

1996-01-01

141

Bubaline herpesvirus 1 associated with abortion in a Mediterranean water buffalo.  

PubMed

During routine analysis of water buffalo foetuses, one sample was positive for herpesvirus and negative to all the other abortive agents investigated. Sequencing of the herpesvirus glycoprotein E gene identified the virus as bubaline herpesvirus 1, showing few differences with the published sequences. This represents the first finding of bubaline herpesvirus in a water buffalo foetus associated with abortion. PMID:23332497

Amoroso, M G; Corrado, F; De Carlo, E; Lucibelli, M G; Martucciello, A; Guarino, A; Galiero, G

2013-06-01

142

PCR detection of cows’ milk in water buffalo milk and mozzarella cheese  

Microsoft Academic Search

Polymerase chain reaction (PCR) has been applied for the specific detection of cows’ DNA in water buffalo milk and mozzarella cheese by using primers targeting the mitochondrial 12S ribosomal RNA gene. The use of specific primers for cow yielded a 346bp fragment from cows’ milk DNA, whereas no amplification signal was obtained in sheep's, goats’ and water buffalo's milk DNA.

I. López-Calleja; I. González Alonso; V. Fajardo; M. A. Rodríguez; P. E. Hernández; T. García; R. Martín

2005-01-01

143

DNA-based vaccines protect against zoonotic schistosomiasis in water buffalo  

Microsoft Academic Search

Schistosomiasis japonica is an endemic, zoonotic disease of major public health importance in China where water buffaloes account for approximately 75% of disease transmission. Interventions that reduce schistosome infection in water buffaloes will enhance their health simultaneously reducing disease transmission to humans. While chemotherapy has proved successful, it requires continued time consuming and expensive mass treatments. A more sustainable option

Akram A. Da’Dara; Yuesheng S. Li; Tie Xiong; Jie Zhou; Gail M. Williams; Donald P. McManus; Zheng Feng; Xin L. Yu; Darren J. Gray; Donald A. Harn

2008-01-01

144

B-cell epitopes recognized by Chinese water buffaloes (Bos buffelus) on the 22 kDa tegumental  

E-print Network

Short Note B-cell epitopes recognized by Chinese water buffaloes (Bos buffelus) on the 22 k / 13-cell epitope / Bos buffelus / Chinese water buffaloes * Correspondence and reprints Tcl.: (61 ) 7 of Schistosol/1a japonicul/1. Ten buffaloes (Bos buffelns) were vaccinated with a recombinant form (reSj-22

Paris-Sud XI, Université de

145

Buffalo Grass as a Means of Water Use Reduction on the UC Davis Campus Call, Siew, Wada, Ware ESM 121 Water Science and Management  

E-print Network

Buffalo Grass as a Means of Water Use Reduction on the UC Davis Campus Call, Siew, Wada, Ware ESM 121 Water Science and Management Buffalo Grass as a Means of Water Use Reduction on the UC Davis if the replacement of turf grass with UC Verde Buffalo Grass could substantially reduce the water use

Pasternack, Gregory B.

146

Effects of Trichostatin A on In Vitro Development and DNA Methylation Level of the Satellite I Region of Swamp Buffalo (Bubalus bubalis) Cloned Embryos  

PubMed Central

Trichostatin A (TSA), a histone deacetylase inhibitor, has been widely used to improve the cloning efficiency in several species. This brings our attention to investigation of the effects of TSA on developmental potential of swamp buffalo cloned embryos. Swamp buffalo cloned embryos were produced by electrical pulse fusion of male swamp buffalo fibroblasts with swamp buffalo enucleated oocytes. After fusion, reconstructed oocytes were treated with 0, 25 or 50 nM TSA for 10 h. The results showed that there was no significant difference in the rates of fusion (82–85%), cleavage (79–84%) and development to the 8-cell stage (59–65%) among treatment groups. The highest developmental rates to the morula and blastocyst stages of embryos were found in the 25 nM TSA-treated group (42.7 and 30.1%, respectively). We also analyzed the DNA methylation level in the satellite I region of donor cells and in in vitro fertilized (IVF) and cloned embryos using the bisulfite DNA sequencing method. The results indicated that the DNA methylation levels in cloned embryos were significantly higher than those of IVF embryos but approximately similar to those of donor cells. Moreover, there was no significant difference in the methylation level among TSA-treated and untreated cloned embryos. Thus, TSA treatments at 25 nM for 10 h could enhance the in vitro developmental potential of swamp buffalo cloned embryos, but no beneficial effect on the DNA methylation level was observed. PMID:24909601

SRIRATTANA, Kanokwan; KETUDAT-CAIRNS, Mariena; NAGAI, Takashi; KANEDA, Masahiro; PARNPAI, Rangsun

2014-01-01

147

Effect of feeding inorganic chromium on growth performance, endocrine variables, and energy metabolites in winter-exposed buffalo calves (Bubalus bubalis).  

PubMed

We investigated the effect of chromium (Cr) supplementation on the growth performance, energy metabolites, and hormonal variation in winter-exposed buffalo calves. Twenty-four female buffalo calves were randomly allotted to four dietary treatments (n?=?6) for a period of 120 days. Feeding regimen was the same in all the groups, except the animals in the four respective groups were additionally supplemented with 0.0, 0.5, 1.0, and 1.5 mg of Cr/kg DM in the form of CrCl3.6H2O. Calves were monitored daily for physiological variables and dry matter intake (DMI). Blood samples were collected at fortnightly intervals from each buffalo calves to measure concentrations of hormones (insulin, cortisol, and growth hormone), energy metabolites (glucose and non-esterified fatty acids), and plasma mineral levels. After 120 days of feeding trial, buffalo calves fed with Cr had lower (P??0.05) among all the treatments. The results suggested that dietary Cr supplementation influenced plasma Cr levels without affecting the plasma concentrations of other trace minerals. However, physiological variables, nutrient intake, and growth performance of buffalo calves did not differ among all treatments (P?>?005). In summary, the current study showed that supplementation of Cr at the level of 1.0 and 1.5 mg of Cr/kg DMI was more effective in improving glucose utilization by increasing potency of insulin hormone and reducing concentration of cortisol hormone. Results also suggested that supplemental Cr also improves blood plasma Cr levels. PMID:24013931

Kumar, Muneendra; Kaur, Harjit; Tyagi, Amrish Kumar; Kewalramani, Neelam J; Mani, Veena; Deka, Rijusmita Sarma; Sharma, Vijay Kumar; Chandra, Gulab; Dang, Ajay Kumar

2013-12-01

148

Genetic characterization of Babesia and Theileria parasites in water buffaloes in Sri Lanka.  

PubMed

Water buffaloes are thought to be the reservoir hosts for several hemoprotozoan parasites that infect cattle. In the present study, we surveyed Sri Lankan bred water buffaloes for infections with Babesia bovis, Babesia bigemina, Theileria annulata, and Theileria orientalis using parasite-specific PCR assays. When 320 blood-derived DNA samples from water buffaloes reared in three different districts (Polonnaruwa, Mannar, and Mullaitivu) of Sri Lanka were PCR screened, B. bovis, B. bigemina, and T. orientalis were detected. While T. orientalis was the predominant parasite (82.5%), low PCR-positive rates were observed for B. bovis (1.9%) and B. bigemina (1.6%). Amplicons of the gene sequences of the Rhoptry Associated Protein-1 (RAP-1) of B. bovis, the Apical Membrane Antigen-1 (AMA-1) of B. bigemina, and the Major Piroplasm Surface Protein (MPSP) of T. orientalis were compared with those characterized previously in Sri Lankan cattle. While the B. bigemina AMA-1 sequences from water buffaloes shared high identity values with those from cattle, B. bovis RAP-1 sequences from water buffaloes diverged genetically from those of cattle. For T. orientalis, none of the MPSP sequence types reported previously in Sri Lankan cattle (types 1, 3, 5, and 7) were detected in the water buffaloes, and the MPSP sequences analyzed in the present study belonged to types N1 or N2. In summary, in addition to reporting the first PCR-based survey of Babesia and Theileria parasites in water buffaloes in Sri Lanka, the present study found that the predominant variants of water buffalo-derived B. bovis RAP-1 and T. orientalis MPSP sequences were different from those previously described from cattle in this country. PMID:24365246

Sivakumar, Thillaiampalam; Tattiyapong, Muncharee; Fukushi, Shintaro; Hayashida, Kyoko; Kothalawala, Hemal; Silva, Seekkuge Susil Priyantha; Vimalakumar, Singarayar Caniciyas; Kanagaratnam, Ratnam; Meewewa, Asela Sanjeewa; Suthaharan, Kalpana; Puvirajan, Thamotharampillai; de Silva, Weligodage Kumarawansa; Igarashi, Ikuo; Yokoyama, Naoaki

2014-02-24

149

Comparison digestibility and protozoa population of Khuzestan water buffalo and Holstein cow  

PubMed Central

The major aim of this study was to compare the morphology and activity of rumen protozoa of Khuzestan water buffalo and Holstein cow using in vitro digestibility and gas production parameters of steam treated sugarcane pith. Rumen fluid obtained from two buffalo and cow steers fed the same diet, 30:70 concentrate: forage. To separate rumen protozoa, antibiotic solution and fungicides were added to rumen fluid. The results of present experiment indicated that the neutral detergent fiber (NDF; 7.8 vs. 1.69%) and acid detergent fiber (ADF; 6.24 vs. 3.24%) digestibility of steam treated sugarcane pith by rumen protozoal population of Khuzestan buffalo was higher than those of cow (p < 0.05). Also, digestibility of dry matter, NDF and ADF by whole buffalo micro-organisms was more than those in cow (p < 0.05). The results indicated that the potential of gas production of sugarcane pith by rumen protozoa in water buffalo was more than that of cow (p < 0.05). Total rumen ciliate protozoa numbers in water buffalo were significantly higher than those of cow (3.68 × 105 vs. 2.18 × 105 mL-1 of rumen content) (p < 0.05). The number of Diplodinium in buffalo was more than that of cow (41.27 vs. 35.7% of total rumen protozoa, respectively). Percentage of Entodinium, Epidinium, Ophryoscolex and Isotricha in cow was more than those of buffalo. Therefore, in the same diet, protozoa and total rumen micro-organisms of Khuzestan water buffalo have higher digestion activity compared to Holstein cow. PMID:25610581

Jabari, Safora; Eslami, Moosa; Chaji, Morteza; Mohammadabadi, Tahereh; Bojarpour, Mohammad

2014-01-01

150

Comparison digestibility and protozoa population of Khuzestan water buffalo and Holstein cow.  

PubMed

The major aim of this study was to compare the morphology and activity of rumen protozoa of Khuzestan water buffalo and Holstein cow using in vitro digestibility and gas production parameters of steam treated sugarcane pith. Rumen fluid obtained from two buffalo and cow steers fed the same diet, 30:70 concentrate: forage. To separate rumen protozoa, antibiotic solution and fungicides were added to rumen fluid. The results of present experiment indicated that the neutral detergent fiber (NDF; 7.8 vs. 1.69%) and acid detergent fiber (ADF; 6.24 vs. 3.24%) digestibility of steam treated sugarcane pith by rumen protozoal population of Khuzestan buffalo was higher than those of cow (p < 0.05). Also, digestibility of dry matter, NDF and ADF by whole buffalo micro-organisms was more than those in cow (p < 0.05). The results indicated that the potential of gas production of sugarcane pith by rumen protozoa in water buffalo was more than that of cow (p < 0.05). Total rumen ciliate protozoa numbers in water buffalo were significantly higher than those of cow (3.68 × 10(5) vs. 2.18 × 10(5) mL(-1) of rumen content) (p < 0.05). The number of Diplodinium in buffalo was more than that of cow (41.27 vs. 35.7% of total rumen protozoa, respectively). Percentage of Entodinium, Epidinium, Ophryoscolex and Isotricha in cow was more than those of buffalo. Therefore, in the same diet, protozoa and total rumen micro-organisms of Khuzestan water buffalo have higher digestion activity compared to Holstein cow. PMID:25610581

Jabari, Safora; Eslami, Moosa; Chaji, Morteza; Mohammadabadi, Tahereh; Bojarpour, Mohammad

2014-01-01

151

Insight into Buffalo (Bubalus bubalis) RIG1 and MDA5 Receptors: A Comparative Study on dsRNA Recognition and In-Vitro Antiviral Response  

PubMed Central

RIG1 and MDA5 have emerged as important intracellular innate pattern recognition receptors that recognize viral RNA and mediate cellular signals controlling Type I interferon (IFN-I) response. Buffalo RIG1 and MDA5 genes were investigated to understand the mechanism of receptor induced antiviral response. Sequence analysis revealed that RIG1 and MDA5 maintain a domain arrangement that is common in mammals. Critical binding site residues of the receptors are evolutionary conserved among mammals. Molecular dynamics simulations suggested that RIG1 and MDA5 follow a similar, if not identical, dsRNA binding pattern that has been previously reported in human. Moreover, binding free energy calculation revealed that MDA5 had a greater affinity towards dsRNA compared to RIG1. Constitutive expressions of RLR genes were ubiquitous in different tissues without being specific to immune organs. Poly I:C stimulation induced elevated expressions of IFN-? and IFN-stimulated genes (ISGs) through interferon regulatory factors (IRFs) mediated pathway in buffalo foetal fibroblast cells. The present study provides crucial insights into the structure and function of RIG1 and MDA5 receptors in buffalo. PMID:24587036

Singh, Manvender; Brahma, Biswajit; Maharana, Jitendra; Patra, Mahesh Chandra; Kumar, Sushil; Mishra, Purusottam; Saini, Megha; De, Bidhan Chandra; Mahanty, Sourav; Datta, Tirtha Kumar; De, Sachinandan

2014-01-01

152

Tuberculosis prevalence and risk factors for water buffalo in Pará, Brazil.  

PubMed

The prevalence of and possible risk factors for tuberculosis were studied in water buffalo from Pará, Brazil. In this study, 3,917 pregnant and nonpregnant female Murrah and Mediterranean buffaloes were studied; 2,089 originated from Marajó Island, and 1,108 were from the mainland. The comparative cervical tuberculin test was used as a diagnostic test for tuberculosis in these animals. The prevalence of positive buffaloes was 3.5 % (100/2,809) on Marajó Island and 7.2 % (80/1,108) on the mainland. The municipalities with the highest tuberculosis prevalence rates in animals were Ipixuna do Pará (10.1 %), Marapanim (9.8 %), Chaves (9.4 %), Paragominas (8.6 %), and Cachoeira do Arari (6.7 %). The tuberculosis prevalence was not significantly different between the Murrah (4.3 %) and Mediterranean (4.8 %) breeds or between pregnant (5 %) and nonpregnant (4.3 %) buffaloes. Tuberculosis was detected in water buffaloes from Pará, Brazil; the mainland buffalo exhibited the highest tuberculosis prevalence. These results indicate that this disease is dangerous to public health and buffalo farming in Pará. PMID:24356890

Barbosa, José D; da Silva, Jenevaldo B; Rangel, Charles P; da Fonseca, Adivaldo H; Silva, Natália S; Bomjardim, Henrique A; Freitas, Nayra F Q R

2014-03-01

153

Effect of Natural and Controlled Climates of the Sahara on Virtual Tritium Space in Friesians and Water Buffaloes  

Microsoft Academic Search

Twenty-eight dry Friesian cows and 33 dry Water Buffalo cows were used in de- termining body water, live body weight, and dry body weight (live body weight -total body water) in winter, spring, and summer. Another four dry Water Buffaloes were exposed to comfortable and hot climates in a climatic laboratory each for one week, when these measure- ments were

T. H. Kamal; S. M. Seif

1969-01-01

154

Separation and quantification of water buffalo milk protein fractions and genetic variants by RP-HPLC.  

PubMed

A RP-HPLC method, developed for the separation and quantification of the most common genetic variants of bovine milk proteins, was successfully applied to the analysis of water buffalo milk. All the most common buffalo casein and whey proteins fractions, as well as their genetic variants, were detected and separated simultaneously in 40 min. Purified buffalo proteins were used as calibration standards and a total of 536 individual milk samples were analysed for protein composition. ?(S1)-, ?(S2)-, ??-, and ?-casein were 32.2%, 15.8%, 36.5%, and 15.5%, respectively, of total casein content, whereas content of ?-Lactoglobulin was approximately 1.3 times as high as that of ?-Lactalbumin. The existence of a polymorphism of ?-casein was demonstrated in Mediterranean water buffalo and ?(S1)- and ?-casein genetic variants were successfully detected by RP-HPLC. PMID:23122071

Bonfatti, Valentina; Giantin, Mery; Rostellato, Roberta; Dacasto, Mauro; Carnier, Paolo

2013-01-15

155

Analysis of genetic variations across regulatory and coding regions of kappa-casein gene of Indian native cattle (Bos indicus) and buffalo (Bubalus bubalis)  

PubMed Central

The promoter region of kappa-casein (?-CN) gene in Indian native cattle and buffalo breeds was sequenced and analyzed for nucleotide variations. Sequence comparison across breeds of Indian cattle revealed a total of 7 variations in the promoter region, of which ? 515 G/T, ? 427 C/T, ? 385 C/T, ? 283 A/G and ? 251 C/T were located within consensus binding sites for octamer-binding protein (OCT1)/pregnancy specific mammary nuclear factor (PMF), activator protein-2 (AP2), hepatocyte nuclear factor (HNF-1) and GAL4 transcription factors (TFs), respectively. These variations might be involved in gain or loss of potential transcription factor binding sites (TFBSs). Unlike the other 4 variants, the ? 283 (A/G) variant located within HNF-1 TFBS was specific to Indian cattle as this change has not been observed in the Bos taurus sequence. Other TFBSs viz., MGF, TBP, NF-1, milk box and C/EBP were conserved across species. For the Indian native buffalo breeds, only 3 changes were identified in the promoter region; ? 305 (A/C), ? 160 (T/C) and ? 141 (A/G) and most of the TFBSs were found to be conserved. However, deletion of two adjacent nucleotides located in and around binding site for C/EBP TF was identified in buffalo when compared with promoter sequence of bovine ?-CN. For ?-CN of Indian native cattle, a strong linkage disequilibrium (LD) was observed for variations 515 G/T, ? 427 C/T and ? 385 C/T in the promoter region; and for variations at codons 136 and 148 of exon-IV. Further, among intragenic haplotypes, variation ? 427 C/T was found to be in LD with variations at codons 136 and 148. The information generated in the present work provides comprehensive characterization of ?-CN gene promoter and coding regions in Indian cattle and buffaloes and reported variations could become important candidates for carrying out further research in dairy traits. PMID:25606460

Kishore, Amit; Mukesh, M.; Sobti, R.C.; Kataria, R.S.; Mishra, B.P.; Sodhi, Monika

2014-01-01

156

25 developmental competence of cloned buffalo (bubalus bubalis) embryos produced by transfected or nontransfected fibroblasts transfer to enucleated oocytes derived from ovum pick-up and abattoir ovaries.  

PubMed

The objective of this study was to explore whether fibroblasts transfection and the source of oocytes - ovum pick-up (OPU) versus abattoir ovaries - affected the in vitro and in vivo developmental competence of somatic cell nuclear transferred (SCNT) embryos in buffalo. To this aim, the serum-starved ear fibroblasts were fused into enucleated oocytes derived from abattoir ovaries (Group 1) and OPU (Group 2). Furthermore, the enucleated buffalo oocytes derived from abattoir ovaries were also fused with pEGFP-N1 transfected ear fibroblasts, and the cloned embryos were enhanced green fluorescent protein (EGFP)-positive confirmed by fluorescence microscopy (Group 3). The reconstructed embryos cultured in Groups 1 to 3 were 262, 83, 120, respectively (5 replicates); and the data were analysed by one-way ANOVA (SPSS Inc., Chicago, IL, USA). As a result, the cleavage rate in Group 3 was significantly higher than that in Group 1 (75.0% v. 54.3%; P<0.01), and the total blastocyst rate of reconstructed embryos in Group 3 (27.3%) was significantly higher than that in Group 1 (17.4%; P<0.01) and Group 2 (24.4%; P<0.05). The SCNT blastocysts were vitrified with 20% ethylene glycol+20% dimethylsulfoxide+0.5M sucrose; the cryosurvival rates of SCNT blastocysts in the 3 groups were not different from each other (90.0%, 94.7%, 92.3%). Following culture, the cryosurvived blastocysts were transferred into synchronized local and crossbred buffaloes, with each recipient receiving 1 or 2 embryos. The pregnancy rates after transferring embryos derived from Groups 1 to 3 were not different from each other, and were 18.75% (3/16), 33.33% (4/12), and 26.67% (4/15), respectively. These results indicate that the oocytes derived from OPU can be enucleated as recipient cytoplasm and transfected fibroblast can be adopted as nuclei donor without decreasing the SCNT efficiency in buffalo. PMID:25472074

Yang, C; Shang, J; Zheng, H; Chen, M; Huang, F; Li, C; Yang, B; Liang, X

2014-12-01

157

Effect of physiologically relevant heat shock on development, apoptosis and expression of some genes in buffalo (Bubalus bubalis) embryos produced in vitro.  

PubMed

For investigating the effects of physiologically relevant heat shock, buffalo oocytes/embryos were cultured at 38.5°C (control) or were exposed to 39.5°C (Group II) or 40.5°C (Group III) for 2 h once every day throughout in vitro maturation (IVM), fertilization (IVF) and culture (IVC). Percentage of oocytes that developed to 8-cell, 16-cell or blastocyst stage was lower (p < 0.05) and the number of apoptotic nuclei was higher (p < 0.05) for Group III > Group II > controls. At both 8-16-cell and blastocyst stages, relative mRNA abundance of stress-related genes HSP 70.1 and HSP 70.2 and pro-apoptotic genes CASPASE-3, BID and BAX was higher (p < 0.05) in Groups III and II than that in controls with the exception of stress-related gene HSF1. Expression level of anti-apoptotic genes BCL-XL and MCL-1 was also higher (p < 0.05) in Groups III and II than that in controls at both 8-16-cell and blastocyst stages. Among the genes related to embryonic development, at 8-16-cell stage, the expression level of GDF9 was higher (p < 0.05) in Group III than that in controls, whereas that of GLUT1, ZAR1 and BMP15 was not significantly different among the three groups. At the blastocyst stage, relative mRNA abundance of GLUT1 and GDF9 was higher (p < 0.05) in Group II than that in controls, whereas that of ZAR-1 and BMP15 was not affected. The results of this study demonstrate that exposure of buffalo oocytes and embryos to elevated temperatures for duration of time that is physiologically relevant severely compromises their developmental competence, increases apoptosis and affects stress-, apoptosis- and development-related genes. PMID:23581430

Yadav, A; Singh, K P; Singh, M K; Saini, N; Palta, P; Manik, R S; Singla, S K; Upadhyay, R C; Chauhan, M S

2013-10-01

158

Transcriptional profile of endometrial TLR4 and 5 genes during the estrous cycle and uterine infection in the buffalo (Bubalus bubalis).  

PubMed

Endometritis is one of the leading causes of infertility in the cattle and buffalo and innate immune mechanism plays an important role in clearing the infection. In this regard, endometrial expression and function of Toll Like Receptors (TLR) are focus of investigation in the recent years. In this study, we report the transcriptional profiles of TLR4 and 5 in the buffalo endometrium during the follicular, early, mid and late luteal phases of estrous cycle and 'subclinical and clinical endometritis' and also at true anestrus (n?=?10 for each stage) using RT-PCR and qRT-PCR as they are the ligands for the lipopolysaccharide and flagellin components of E.coli, the most common cause of postpartum endometritis. We found a significant positive correlation between TLR4 and 5 in all the groups (r?=?0.696-0.803; P??0.05). Chi-square analysis showed that the qualitative expression of endometrial TLR4 and 5 transcripts was significantly associated with the phase of estrous cycle and also with uterine infection (P?

Ajevar, Ganesan; Muthu, Sankar; Sarkar, Mihir; Kumar, Harendra; Das, Goutam Kumar; Krishnaswamy, Narayanan

2014-06-01

159

Review Water Buffalo Genome Science Comes of Age International Journal of Biological Sciences  

E-print Network

The water buffalo is vital to the lives of small farmers and to the economy of many countries worldwide. Not only are they draught animals, but they are also a source of meat, horns, skin and particularly the rich and precious milk that may be converted to creams, butter, yogurt and many cheeses. Genome analysis of water buffalo has advanced significantly in recent years. This review focuses on currently available genome resources in water buffalo in terms of cytogenetic characterization, whole genome mapping and next generation sequencing. No doubt, these resources indicate that genome science comes of age in the species and will provide knowledge and technologies to help optimize production potential, reproduction efficiency, product quality, nutritional value and resistance to diseases. As water buffalo and domestic cattle, both members of the Bovidae family, are closely related, the vast amount of cattle genetic/genomic resources might serve as shortcuts for the buffalo community to further advance genome science and biotechnologies in the species.

Vanessa N. Michelizzi; Michael V. Dodson; Zengxiang Pan; M Elisabete; J Amaral; Jennifer J. Michal; Derek J. Mclean; James E. Womack; Zhihua Jiang

2010-01-01

160

Comparative study on the rumen ciliate populations in small experimental herds of water buffalo and Kedah Kelantan cattle in Malaysia  

Microsoft Academic Search

Seventeen ciliate species were identified from water buffalo and Kedah Kelantan cattle in Malaysia. Although most of these species have been detected from domestic ruminants in tropical and temperate areas, the presence of Metadinium ypsilon and Ostracodinium trivesiculatum is characteristic of Malaysian cattle and water buffalo. The average numbers of ciliates per milliliter of rumen contents were similar to those

S. Imai; N. Abdullah; Y. W. Ho; S. Jalaludin; H. Y. Hussain; R. Onodera; H. Kudo

1995-01-01

161

High prevalence of muscular sarcocystosis in cattle and water buffaloes from Selangor, Malaysia.  

PubMed

The prevalence of sarcocystosis in cattle and water buffaloes from peninsular Malaysia was investigated in abattoirs in Selangor state, February, 2011, to March, 2012. Fresh muscle samples were collected from the tongue, heart, oesophagus, diaphragm and skeletal muscles of 102 cattle and 18 water buffaloes. Each sample was initially screened by light microscopy and then fixed for further histopathological analysis. Out of 120 animals examined, 49 (40.8%) harboured the microscopic type of Sarcocystis spp. The positivity rate for cattle was 36.2% and for water buffaloes 66.7%. In cattle, the organs highly infected were the skeletal muscles and diaphragm (27% each), followed by tongue and esophagus (24.3% each), and the heart (8%). In water buffaloes, the heart was most often infected (66.7%), followed by the oesophagus (50%) and skeletal muscle (33.3%); no sarcocysts were detected in the tongue and diaphragm. The shape of the sarcocyst was fusiform to oval with a mean cyst size of 151.66 x 75.83 ?m and wall thickness of 2.47 ?m in cattle, and 114 x 50.81 ?m cyst size and the wall thickness of 1.11 ?m in water buffaloes, consistent with Sarcocystis cruzi and Sarcocystis levinei, respectively. Remaining tissue from cattle was subjected to parasite specific 18S rRNA gene PCR and Sarcocystis cruzi was confirmed, at least exemplarily. The peripheral metrocytes and the banana-shaped bradyzoites (15.23 x 2.2 ?m in cattle and 11.49 x 2.45 ?m in water buffalo hosts) were easily recognized. In conclusion, a high positivity rate was found in Malaysian meat-producing animals with possible implications for meat consumption and human health. PMID:24522140

Latif, B; Vellayan, S; Heo, C C; Kannan Kutty, M; Omar, E; Abdullah, S; Tappe, D

2013-12-01

162

Infection of water buffalo in Rio de Janeiro Brazil with Anaplasma marginale strains also reported in cattle.  

PubMed

Anaplasma marginale is the most prevalent pathogen of cattle in tropical and subtropical regions of the world and causes the disease bovine anaplasmosis. The importance of water buffalo in the world economy is increasing. In addition, while water buffalo may serve as a reservoir host for A. marginale, the susceptibility of this host for A. marginale cattle strains in Brazil has not been reported. The major surface protein 1 alpha (msp1?) gene has been shown to be a stable genetic marker for identification of A. marginale strains. Herein, we analyzed blood samples from 200 water buffalo and identified the A. marginale strains in an endemic area of Rio de Janeiro, Brazil, where ticks were present and water buffalo and cattle co-mingled. Ticks that were feeding on the study buffalo were collected and identified. The prevalence of A. marginale in water buffalo in this study was low (10%). Sequence analysis of the msp1? gene demonstrated the presence of 8 different A. marginale strains. Two A. marginale strains in the water buffalo, (?-?-?-?-?) and (?-?-?-?), were similar to those reported in cattle from nearby regions. The results of this study suggested that water buffalo in this region are naturally infected with the same strains of A. marginale found in cattle. PMID:25260335

Silva, Jenevaldo B; Cabezas-Cruz, Alejandro; Fonseca, Adivaldo H; Barbosa, José D; de la Fuente, José

2014-10-15

163

Seasonal variation in follicular dynamics of superovulated Indian water buffalo  

Microsoft Academic Search

The ovaries of 5 buffalo were examined daily by ultrasound beginning at Day 3 of the estrous cycle, followed by superovulation between Days 11 and 13 of the cycle in both the wet cool and dry hot seasons. Daily ultrasonographic observations of the ovaries were recorded on a videotape and were used to assess the progression of both the large

M. Taneja; S. M. Totey; A. Ali

1995-01-01

164

Quantitative expression of pluripotency-related genes in parthenogenetically produced buffalo (Bubalus bubalis) embryos and in putative embryonic stem cells derived from them.  

PubMed

Parthenogenetically produced embryos and embryonic stem (ES) cells derived from them offer a unique model for investigating the role of transcription factors in embryonic genome activation (EGA), pluripotent lineage specification and in pluripotency and self-renewal of ES cells because of the unique nature of these embryos. There is little information on the quantitative expression of important genes in parthenogenetically produced embryos and in ES cells derived from them. The present study examined the quantitative expression of some important genes in parthenogenetically produced buffalo embryos and in putative parthenogenetic ES cells (pES) cells. The quantitative expression of OCT-4, SOX-2, NANOG, REX-1, FOXD-3 and NUCLEOSTEMIN, which is very low in immature and mature oocytes, and in embryos at 2-, 4- and 8- to 16-cell stage, increases significantly at morula and blastocyst stage. The expression level of TELOMERASE, c-MYC and STAT-3, which is high in immature oocytes decreases during embryonic development followed by either an increase at the morula stage (TELOMERASE) or a low expression level maintained throughout development till blastocyst stage (c-MYC and STAT-3). There is a progressive decline in the expression level of OCT-4, SOX-2, c-MYC, REX-1, NUCLEOSTEMIN, TELOMERASE and STAT-3 during long term culture of pES cells. PMID:25077841

Singh, K P; Kaushik, R; Mohapatra, S K; Garg, V; Rameshbabu, K; Singh, M K; Palta, P; Manik, R S; Singla, S K; Chauhan, M S

2014-09-01

165

Prevalence Survey of Selected Bovine Pathogens in Water Buffaloes in the North Region of Brazil  

PubMed Central

Although the largest buffalo herd in the occident is in the north region of Brazil, few studies have been conducted to assess the prevalence of selected parasitic diseases in buffalo herd. The present study was therefore conducted to investigate the epidemiological of Toxoplasma gondii, Neospora caninum, Anaplasma marginale, Babesia bigemina, and Babesia bovis in water buffaloes in the north region of Brazil. A total of 4796 buffalo blood samples were randomly collected from five provinces and simultaneously analyzed by the IFAT and ELISA. The serological prevalence of T. gondii and N. caninum was 41.3% and 55.5% in ELISA and 35.7% and 48.8% in IFAT, respectively. The overall prevalence of A. marginale, B. bovis, and B. bigemina was 63%, 25%, and 21% by ELISA and 50.0%, 22.5%, and 18.8% by IFAT, respectively. This study shows valuable information regarding the serological survey of selected bovine pathogens in water buffaloes in the north region of Brazil which will likely be very beneficial for the management and control programs of this disease. PMID:24563780

da Silva, Jenevaldo Barbosa; dos Santos, Priscilla Nunes; de Santana Castro, Gustavo Nunes; da Fonseca, Adivaldo Henrique; Barbosa, José Diomedes

2014-01-01

166

Bovine tuberculosis in African buffaloes: observations regarding Mycobacterium bovis shedding into water and exposure to environmental mycobacteria  

Microsoft Academic Search

BACKGROUND: African buffaloes are the maintenance host for Mycobacterium bovis in the endemically infected Kruger National Park (KNP). The infection is primarily spread between buffaloes via the respiratory route, but it is not known whether shedding of M. bovis in nasal and oral excretions may lead to contamination of ground and surface water and facilitate the transmission to other animal

Anita L Michel; Lin-Mari de Klerk; Nico C Gey van Pittius; Rob M Warren; Paul D van Helden

2007-01-01

167

Effect of dietary protein on intake, nutrients utilization, nitrogen balance, blood metabolites, growth and puberty in growing Bhadawari buffalo (Bubalus bubalis) heifers.  

PubMed

Fifteen Bhadawari buffalo heifers of 207?±?9.78 kg mean body weight were randomly distributed into three dietary groups to evaluate the effect of protein level on nutrient utilization, nitrogen (N) balance, growth rate, blood metabolites, and puberty. All animals were offered wheat straw-berseem diets supplemented with concentrate mixtures of similar energy (2.7 Mcal/kg) and different protein levels (14.3-22 %). Animals of standard-protein group (SPG) were offered protein and energy as per requirement, while animals of low-protein group (LPG) and high-protein group (HPG) were fed 20 % less and 20 % more protein, respectively, than SPG. Feed dry matter (DM) and metabolizable energy (ME) intake (% body wt. and g/kg w(0.75)) were similar for all three diets; however, the crude protein (CP) and digestible crude protein (DCP) intake on percent body weight and per kilogram metabolic weight was higher (P??0.05) in SPG (330.8 g/day) than in LPG (296.7 g/day), while the animals gained more weight in January to March months and the lowest weight in May to July months. Protein level had no effect on conception rate of heifers. Results revealed that 20 % higher or less protein than the ICAR requirement had no significant (P?>?0.05) on feed intake, nutrient conversion efficiency for weight gain, heifer growth, and puberty; however, 20 % more protein increased urinary N loss. PMID:25407740

Singh, Sultan; Kushwaha, Badri Prasad; Maity, Subendu Bikas; Singh, Krishan Kunwar; Das, Nityanand

2015-01-01

168

Differential Expression of Th1- and Th2- Type Cytokines in Peripheral Blood Mononuclear Cells of Murrah Buffalo (Bubalus Bubalis) on TLR2 Induction by B. Subtilis Peptidoglycan  

PubMed Central

Peripheral blood mononuclear cells (PBMCs) discriminate microbial pathogens and induce T-cell responses of appropriate effector phenotype accordingly. Toll-like receptors (TLRs), in part, mediate this microbial recognition and differentiation while the development of T-cell effector functions critically depends on the release of Th1- or Th2- type cytokines. In the present study, buffalo PBMCs were stimulated under in vitro culture conditions by Bacillus subtilis cell wall petidoglycan, a TLR2 ligand, in a dose-and time- dependent manner. The expression of TLR2 as well as the subsequent differential induction of the Th1 and Th2 type cytokines was measured. Stimulation was analyzed across five doses of peptidoglycan (10 ?/ml, 20 ?g/ml, 30 ?g/ml, 40 ?g/ml and 50 ?g/ml) for 3 h, 12 h, 24 h and 36 h incubation periods. We observed the induction of TLR2 expression in a dose- and time-dependent manner and the peptidoglycan induced tolerance beyond 30 ?g/ml dose at all incubation periods. The correlation between peptidoglycan stimulation and TLR2 induction was found positive at all doses and for all incubation periods. Increased production of all the cytokines was observed at low doses for 3 h incubation, but the expression of IL-4 was relatively higher than IL-12 at the higher antigen doses, indicating tailoring towards Th2 response. At 12 h incubation, there was a pronounced decrease in IL-4 and IL-10 expression relative to IL-12 in a dose- dependent manner, indicating skewing to Th1 polarization. The expression of IL-12 was highest for all doses across all the incubation intervals at 24 h incubation, indicating Th1 polarization. The relative expression of TNF-? and IFN-? was also higher while that of IL-4 and IL-10 showed a decrease. For 36 h incubation, at low doses, relative increase in the expression of IL-4 and IL-10 was observed which decreased at higher doses, as did the expression of all other cytokines. The exhaustion of cytokine production at 36 h indicated that PBMCs became refractory to further stimulation. It can be concluded from this study that the cytokine response to sPGN initially was of Th2 type which skews, more pronouncedly, to Th1 type with time till the cells become refractory to further stimulation. PMID:25049659

Shah, Syed M.; Ravi Kumar, G. V. P. P. S.; Brah, G. S.; Santra, Lakshman; Pawar, Hitesh

2012-01-01

169

Comparison of serological tests for Trypanosoma evansi natural infections in water buffaloes from north Vietnam  

Microsoft Academic Search

In the present study, a collection of 415 water buffalo serum samples originating from the north of Vietnam was used for evaluation of different diagnostic antibody detection methods available to detect infections with Trypanosoma evansi. The diagnostic sensitivity and specificity of a direct card agglutination test (CATT\\/T. evansi), an indirect card agglutination test (LATEX\\/T. evansi) and a newly developed antibody

D Verloo; W Holland; L. N My; N. G Thanh; P. T Tam; B Goddeeris; J Vercruysse; P Büscher

2000-01-01

170

Application of Toxicity Identification Evaluation Techniques to Pore Water from Buffalo River Sediments  

Microsoft Academic Search

To identify contaminants responsible for toxicity of sediments from the Buffalo River, toxicity identification evaluations (TIEs) were conducted with interstitial (pore) water from several sites. Initial toxicity of the samples was determined using fathead minnows (Pimephales promelas) and the cladoceran Ceriodaphnia dubia, and TIE analyses were conducted with the most sensitive of the two species at a particular site. Fathead

Gerald T. Ankley; Mary K. Schubauer-Berigan; Joseph R. Dierkes

1996-01-01

171

Toll-like receptor responses to Peste des petits ruminants virus in goats and water buffalo.  

PubMed

Ovine rinderpest or goat plague is an economically important and contagious viral disease of sheep and goats, caused by the Peste des petits ruminants virus (PPRV). Differences in susceptibility to goat plague among different breeds and water buffalo exist. The host innate immune system discriminates between pathogen associated molecular patterns and self antigens through surveillance receptors known as Toll like receptors (TLR). We investigated the role of TLR and cytokines in differential susceptibility of goat breeds and water buffalo to PPRV. We examined the replication of PPRV in peripheral blood mononuclear cells (PBMC) of Indian domestic goats and water buffalo and demonstrated that the levels of TLR3 and TLR7 and downstream signalling molecules correlation with susceptibility vs resistance. Naturally susceptible goat breeds, Barbari and Tellichery, had dampened innate immune responses to PPRV and increased viral loads with lower basal expression levels of TLR 3/7. Upon stimulation of PBMC with synthetic TLR3 and TLR7 agonists or PPRV, the levels of proinflammatory cytokines were found to be significantly higher while immunosuppressive interleukin (IL) 10 levels were lower in PPRV resistant Kanni and Salem Black breeds and water buffalo at transcriptional level, correlating with reduced viralloads in infected PBMC. Water buffalo produced higher levels of interferon (IFN) ? in comparison with goats at transcriptional and translational levels. Pre-treatment of Vero cells with human IFN? resulted in reduction of PPRV replication, confirming the role of IFN? in limiting PPRV replication. Treatment with IRS66, a TLR7 antagonist, resulted in the reduction of IFN? levels, with increased PPRV replication confirming the role of TLR7. Single nucleotide polymorphism analysis of TLR7 of these goat breeds did not show any marked nucleotide differences that might account for susceptibility vs resistance to PPRV. Analyzing other host genetic factors might provide further insights on susceptibility to PPRV and genetic polymorphisms in the host. PMID:25369126

Dhanasekaran, Sakthivel; Biswas, Moanaro; Vignesh, Ambothi R; Ramya, R; Raj, Gopal Dhinakar; Tirumurugaan, Krishnaswamy G; Raja, Angamuthu; Kataria, Ranjit S; Parida, Satya; Subbiah, Elankumaran

2014-01-01

172

Toll-Like Receptor Responses to Peste des petits ruminants Virus in Goats and Water Buffalo  

PubMed Central

Ovine rinderpest or goat plague is an economically important and contagious viral disease of sheep and goats, caused by the Peste des petits ruminants virus (PPRV). Differences in susceptibility to goat plague among different breeds and water buffalo exist. The host innate immune system discriminates between pathogen associated molecular patterns and self antigens through surveillance receptors known as Toll like receptors (TLR). We investigated the role of TLR and cytokines in differential susceptibility of goat breeds and water buffalo to PPRV. We examined the replication of PPRV in peripheral blood mononuclear cells (PBMC) of Indian domestic goats and water buffalo and demonstrated that the levels of TLR3 and TLR7 and downstream signalling molecules correlation with susceptibility vs resistance. Naturally susceptible goat breeds, Barbari and Tellichery, had dampened innate immune responses to PPRV and increased viral loads with lower basal expression levels of TLR 3/7. Upon stimulation of PBMC with synthetic TLR3 and TLR7 agonists or PPRV, the levels of proinflammatory cytokines were found to be significantly higher while immunosuppressive interleukin (IL) 10 levels were lower in PPRV resistant Kanni and Salem Black breeds and water buffalo at transcriptional level, correlating with reduced viralloads in infected PBMC. Water buffalo produced higher levels of interferon (IFN) ? in comparison with goats at transcriptional and translational levels. Pre-treatment of Vero cells with human IFN? resulted in reduction of PPRV replication, confirming the role of IFN? in limiting PPRV replication. Treatment with IRS66, a TLR7 antagonist, resulted in the reduction of IFN? levels, with increased PPRV replication confirming the role of TLR7. Single nucleotide polymorphism analysis of TLR7 of these goat breeds did not show any marked nucleotide differences that might account for susceptibility vs resistance to PPRV. Analyzing other host genetic factors might provide further insights on susceptibility to PPRV and genetic polymorphisms in the host. PMID:25369126

Dhanasekaran, Sakthivel; Biswas, Moanaro; Vignesh, Ambothi R.; Ramya, R.; Raj, Gopal Dhinakar; Tirumurugaan, Krishnaswamy G.; Raja, Angamuthu; Kataria, Ranjit S.; Parida, Satya; Subbiah, Elankumaran

2014-01-01

173

Identity and public health potential of Cryptosporidium spp. in water buffalo calves in Egypt.  

PubMed

Little is known about the diversity and public health significance of Cryptosporidium species in water buffaloes. In this study, we examined the distribution of Cryptosporidium spp. in water buffalo calves in Egypt. Rectal fecal specimens from 179 calves and 359 adults were screened microscopically for Cryptosporidium oocysts using modified Ziehl-Neelsen stain. Cryptosporidium spp. in 17 microscopy-positive specimens from calves were genotyped by DNA sequence analysis of the small-subunit rRNA gene, and Cryptosporidium parvum was subtyped by sequence analysis of the 60 kDa glycoprotein gene. Cryptosporidium ryanae was found in 10 specimens and C. parvum in 7 specimens, with the former belonging to the newly identified C. ryanae buffalo variant and the latter belonging to the subtypes IIdA20G1 (in 5 specimens) and IIaA15G1R1 (in 2 specimens). The prevailing occurrence of C. ryanae and the subtype family IId of C. parvum and the absence of C. bovis and C. andersoni represent some features of Cryptosporidium transmission in water buffaloes in Egypt. PMID:22963712

Amer, Said; Zidan, Shereif; Feng, Yaoyu; Adamu, Haileeyesus; Li, Na; Xiao, Lihua

2013-01-16

174

Water buffalo genome characterization by the Illumina BovineHD BeadChip.  

PubMed

To define the best strategies for genomic association studies and genomic selection, it is necessary to determine the extent of linkage disequilibrium (LD) and the genetic structure of the study population. The current study evaluated the transference of genomic information contained in the Illumina BovineHD BeadChip from cattle to buffaloes, and assessed the extent of the LD in buffaloes. Of the 688,593 bovine single nucleotide polymorphism (SNP) that were successfully genotyped from the 384 buffalo samples, only 16,580 markers were polymorphic, and had minor allele frequencies greater than 0.05. A total of 16,580 polymorphic SNPs were identified, which were uniformly distributed throughout the autosomes, because the density and mean distance between markers were similar for all autosomes. The average minor allele frequency for the 16,580 SNPs was 0.23. The overall mean LD for pairs of adjacent markers was 0.29 and 0.71, when measured as for r2 and |D'|, respectively. The 16,580 polymorphic SNPs were matched to Bos taurus chromosome in the current bovine genome assembly (Btau 4.2), and could be utilized in association studies. In conclusion, the Illumina BovineHD BeadChip contains approximately 16,580 polymorphic markers for the water buffalo, which are broadly distributed across the genome. These data could be used in genomic association and genomic selection studies; however, it might be necessary to develop a panel with specific SNP markers for water buffaloes. PMID:25036164

Borquis, R R A; Baldi, F; de Camargo, G M F; Cardoso, D F; Santos, D J A; Lugo, N H; Sargolzaei, M; Schenkel, F S; Albuquerque, L G; Tonhati, H

2014-01-01

175

Investigation of transferability of BovineSNP50 BeadChip from cattle to water buffalo for genome wide association study.  

PubMed

Cattle and water buffalo belong to the same subfamily Bovinae and share chromosome banding and gene order homology. In this study, we used genome-wide Illumina BovineSNP50 BeadChip to analyze 91 DNA samples from three breeds of water buffalo (Nili-Ravi, Murrah and their crossbred with local GuangXi buffalos in China), to demonstrate the genetic divergence between cattle and water buffalo through a large single nucleotide polymorphism (SNP) transferability study at the whole genome level, and performed association analysis of functional traits in water buffalo as well. A total of 40,766 (75.5 %) bovine SNPs were found in the water buffalo genome, but 49,936 (92.5 %) were with only one allele, and finally 935 were identified to be polymorphic and useful for association analysis in water buffalo. Therefore, the genome sequences of water buffalo and cattle shared a high level of homology but the polymorphic status of the bovine SNPs varied between these two species. The different patterns of mutations between species may associate with their phenotypic divergence due to genome evolution. Among 935 bovine SNPs, we identified a total of 9 and 7 SNPs significantly associated to fertility and milk production traits in water buffalo, respectively. However, more works in larger sample size are needed in future to verify these candidate SNPs for water buffalo. PMID:23232712

Wu, Jun Jing; Song, Li Jun; Wu, Fang Jie; Liang, Xian Wei; Yang, Bing Zhuang; Wathes, D Claire; Pollott, Geoff E; Cheng, Zhangrui; Shi, De Shun; Liu, Qing You; Yang, Li Guo; Zhang, Shu Jun

2013-02-01

176

Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes.  

PubMed

The genes that encode merozoite surface antigens (MSAs) in Babesia bovis are genetically diverse. In this study, we analyzed the genetic diversity of B. bovis MSA-1, MSA-2b, and MSA-2c genes in Vietnamese cattle and water buffaloes. Blood DNA samples from 258 cattle and 49 water buffaloes reared in the Thua Thien Hue province of Vietnam were screened with a B. bovis-specific diagnostic PCR assay. The B. bovis-positive DNA samples (23 cattle and 16 water buffaloes) were then subjected to PCR assays to amplify the MSA-1, MSA-2b, and MSA-2c genes. Sequencing analyses showed that the Vietnamese MSA-1 and MSA-2b sequences are genetically diverse, whereas MSA-2c is relatively conserved. The nucleotide identity values for these MSA gene sequences were similar in the cattle and water buffaloes. Consistent with the sequencing data, the Vietnamese MSA-1 and MSA-2b sequences were dispersed across several clades in the corresponding phylogenetic trees, whereas the MSA-2c sequences occurred in a single clade. Cattle- and water-buffalo-derived sequences also often clustered together on the phylogenetic trees. The Vietnamese MSA-1, MSA-2b, and MSA-2c sequences were then screened for recombination with automated methods. Of the seven recombination events detected, five and two were associated with the MSA-2b and MSA-2c recombinant sequences, respectively, whereas no MSA-1 recombinants were detected among the sequences analyzed. Recombination between the sequences derived from cattle and water buffaloes was very common, and the resultant recombinant sequences were found in both host animals. These data indicate that the genetic diversity of the MSA sequences does not differ between cattle and water buffaloes in Vietnam. They also suggest that recombination between the B. bovis MSA sequences in both cattle and water buffaloes might contribute to the genetic variation in these genes in Vietnam. PMID:25575442

Yokoyama, Naoaki; Sivakumar, Thillaiampalam; Tuvshintulga, Bumduuren; Hayashida, Kyoko; Igarashi, Ikuo; Inoue, Noboru; Long, Phung Thang; Lan, Dinh Thi Bich

2015-03-01

177

Serological survey of antibodies to Toxoplasma gondii in goats, sheep, cattle and water buffaloes in Bahia State, Brazil  

Microsoft Academic Search

Serum samples from 439 goats, 240 sheep, 194 cattle and 104 water buffaloes were tested for antibodies to Toxoplasma gondii by a latex agglutination test. Antibodies to T. gondii were found in 28.93% of goats, 18.75% of sheep, 1.03% of cattle and 3.85% of water buffaloes, at a dilution of ?1:64. The highest titres observed in goats, sheep, cattle and

L. F. Pita Gondim; H. V. Barbosa; C. H. A. Ribeiro Filho; H. Saeki

1999-01-01

178

The utility of polymerase chain reaction for diagnosis of lumpy skin disease in cattle and water buffaloes in Egypt.  

PubMed

An outbreak of lumpy skin disease (LSD) occurred among cattle and water buffaloes in Egypt in 2006. Polymerase chain reaction (PCR) and the agar gel precipitation test (AGPT) were compared. Eight of ten (80%) tissue specimens from diseased cattle were positive with AGPT while 100% were positive with PCR. Of ten tissue specimens from diseased water buffaloes, 70% were positive with AGPT while 100% were positive with PCR. Ten milk samples were obtained from diseased water buffaloes; PCR detected nucleic acid of LSD virus (LSDV) in 50% while AGPT failed to detect LSDV antigen. Water buffaloes are susceptible to LSDV infection. The clinical signs of LSD were less severe in water buffaloes, but the virus was excreted in their milk. Diagnosis of LSD outbreaks by PCR will facilitate rapid application of control measures. Mass vaccination should be applied in both cattle and water buffaloes in Egypt using an effective specific vaccine against LSD, such as the attenuated Neethling strain vaccine or a recombinant vaccine. PMID:22435194

Sharawi, S S A; Abd El-Rahim, I H A

2011-12-01

179

Underway view from port side in the Buffalo River with ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Underway view from port side in the Buffalo River with water cannons spraying. City of Buffalo in background. TC - Fireboat EDWARD M. COTTER, Moored on the Buffalo River at 155 Ohio Street, Buffalo, Erie County, NY

180

Low genetic diversity associated with low prevalence of Anaplasma marginale in water buffaloes in Marajó Island, Brazil.  

PubMed

The rickettsia Anaplasma marginale is the etiologic agent of bovine anaplasmosis, an important tick-borne disease affecting cattle in tropical and subtropical regions of the world. In endemic regions, the genetic diversity of this pathogen is usually related to the high prevalence of the disease in cattle. The major surface protein 1 alpha (MSP1a) has been used as a marker to characterize the genetic diversity and for geographical identification of A. marginale strains. The present study reports the characterization of A. marginale MSP1a diversity in water buffaloes. Blood samples were collected from 200 water buffaloes on Marajó Island, Brazil where the largest buffalo herd is located in the Western hemisphere. Fifteen buffaloes (7.5%) were positive for A. marginale msp1? by PCR. Four different strains of A. marginale with MSP1a tandem repeat structures (4-63-27), (162-63-27), (78-24-24-25-31) and (?-10-10-15) were found, being (4-63-27) the most common. MSP1a tandem repeats composition in buffalos and phylogenetic analysis using msp1? gene showed that the A. marginale strains identified in buffaloes are closely related to A. marginale strains from cattle. The results demonstrated low genetic diversity of A. marginale associated with low bacterial prevalence in buffaloes and suggested that buffaloes may be reservoirs of this pathogen for cattle living in the same area. The results also suggested that mechanical transmission and not biological transmission by ticks might be playing the major role for pathogen circulation among water buffaloes in Marajó Island, Brazil. PMID:25108778

Silva, Jenevaldo B; Fonseca, Adivaldo H; Barbosa, José D; Cabezas-Cruz, Alejandro; de la Fuente, José

2014-10-01

181

Characterization of CTLA-4, PD-1 and PDL-1 of swamp and riverine type water buffaloes.  

PubMed

Characterization of CTLA-4, PD-1 and PDL-1 genes from swamp and riverine type water buffaloes was done by molecular cloning, sequencing and phylogenetic analysis. The cloned cDNA of CTLA-4, PD-1 and PDL-1 contained an open reading frame of 666, 849 and 870 nucleotides, encoding a polypeptide of 221, 282 and 298 amino acids, respectively. Nucleotide sequence homology of both CTLA-4 and PDL-1 had 99.8% in swamp and riverine type, which gives the identical polypeptide. Meanwhile, PD-1 genes of swamp and riverine type water buffaloes had 99.2% of homology in nucleotide sequence, which has substitution of two amino acid residues. The hexapeptide motif, phosphatidylinositol 3'-kinase and potential glycosylation sites were conserved within the tribe Bovinae. Phylogenetic analysis confirmed the degree of relationship between the bubaline species and justify the distinctness of each breeds by the bootstrap value generated. PMID:20334916

Mingala, Claro N; Konnai, Satoru; Ikebuchi, Ryoyo; Ohashi, Kazuhiko

2011-01-01

182

Link between Geographical Origin and Occurrence of Brucella abortus Biovars in Cow and Water Buffalo Herds  

PubMed Central

Sixty-three Brucella isolates from water buffaloes and cattle slaughtered within the Italian national plan for brucellosis control were characterized by multiple-locus variable-number tandem repeat analysis (MLVA). Genotyping indicated a strong influence of geographic origin on the Brucella abortus biovar distribution in areas where brucellosis is endemic and highlighted the importance of rigorous management procedures aimed at avoiding inter- and intraherd spreading of pathogens. PMID:23183984

Peletto, Simone; Lucibelli, Maria G.; Acutis, Pier L.; Ercolini, Danilo; Galiero, Giorgio

2013-01-01

183

Link between geographical origin and occurrence of Brucella abortus biovars in cow and water buffalo herds.  

PubMed

Sixty-three Brucella isolates from water buffaloes and cattle slaughtered within the Italian national plan for brucellosis control were characterized by multiple-locus variable-number tandem repeat analysis (MLVA). Genotyping indicated a strong influence of geographic origin on the Brucella abortus biovar distribution in areas where brucellosis is endemic and highlighted the importance of rigorous management procedures aimed at avoiding inter- and intraherd spreading of pathogens. PMID:23183984

Borriello, Giorgia; Peletto, Simone; Lucibelli, Maria G; Acutis, Pier L; Ercolini, Danilo; Galiero, Giorgio

2013-02-01

184

Cloning of Taiwan water buffalo male-specific DNA sequence for sexing  

Microsoft Academic Search

Random amplified polymorphic DNA (RAPD) fingerprinting was carried out to investigate the sex-specific DNA sequence for sexing in Taiwan water buffalos. One hundred and forty random primers were used for RAPD-PCR (polymerase chain reaction). One of these primers, OPC-16, produced a 321bp fragment found only in tested males. This male-specific fragment was isolated and constructed into plasmids for nucleotide sequencing,

Yan-Ming Horng; Yi-Ting Chen; Chean-Ping Wu; Yu-Shine Jea; Mu-Chiou Huang

2004-01-01

185

Differential scanning calorimetry of water buffalo and cow milk fat in mozzarella cheese  

Microsoft Academic Search

The thermal profiles of the fat in mozzarella cheeses made from cow milk (CM) and water buffalo milk (WBM) were obtained by\\u000a differential scanning calorimetry (DSC). The DSC curves of mozzarella cheese made from WBM were distinguishable from those\\u000a of CM. The curves resembled those of the corresponding milk fats and could be divided into low-, medium-, and high-temperature\\u000a melting

Michael H. Tunick; Edyth L. Malin

1997-01-01

186

Schistosoma japonicum: An ultraviolet-attenuated cercarial vaccine applicable in the field for water buffaloes  

Microsoft Academic Search

Water buffaloes were vaccinated three times with 10,000 Schistosoma japonicum cercariae irradiated with ultraviolet (uv) light at a dose of 400 microW x min\\/cm2. The irradiation was performed with cheap, simple, and portable equipment in a rural area of Hubei Province (People's Republic of China). A challenge infection of 1000 untreated cercariae was given to six vaccinated and six naive

You-en Shi; Chang-Fu Jiang; Jia-Jun Han; Yong-Long Li; Andreas Ruppel

1990-01-01

187

77 FR 39408 - Safety Zone; Buffalo July 4th Fireworks, Lake Erie, Buffalo, NY  

Federal Register 2010, 2011, 2012, 2013

...This zone will encompass all waters of Lake Erie, Buffalo, NY within a 1680 foot radius...4th Fireworks, Lake Erie, Buffalo, NY. (a) Location. This zone will encompass all waters of Lake Erie, Buffalo, NY within a 1680 foot...

2012-07-03

188

Schistosoma japonicum: An ultraviolet-attenuated cercarial vaccine applicable in the field for water buffaloes  

SciTech Connect

Water buffaloes were vaccinated three times with 10,000 Schistosoma japonicum cercariae irradiated with ultraviolet (uv) light at a dose of 400 microW x min/cm2. The irradiation was performed with cheap, simple, and portable equipment in a rural area of Hubei Province (People's Republic of China). A challenge infection of 1000 untreated cercariae was given to six vaccinated and six naive control buffaloes, while two vaccinated animals were not challenged. The experiment was terminated 6 weeks after the challenge. Control animals had lost body weight and harbored a mean of 110 worms and 37 eggs per gram of liver. The vaccinated animals gained weight after the challenge and developed 89% resistance to infection with S. japonicum. Since schistosomiasis japonica is nowadays transmitted in China predominantly by domestic livestock, a uv-attenuated cercarial vaccine for bovines may contribute to the control of this disease.

Shi, Y.E.; Jiang, C.F.; Han, J.J.; Li, Y.L.; Ruppel, A. (Tongii Medical Univ., Wuhan, Hubei Province (China))

1990-07-01

189

Habitat Suitability Index Models: Smallmouth Buffalo  

USGS Publications Warehouse

This is one of a series of publications that provide information on the habitat requirements of selected fish and wildlife species. Literature describing the relationship between habitat variables related to life requisites and habitat suitability for the Smallmouth buffalo (Ictiobus bubalus) are synthesized. These data are subsequently used to develop Habitat Suitability (HIS) models. The HSI models are designed to provide information that can be used in impact assessment and habitat management.

Edwards, Elizabeth A.; Twomey, Katie

1982-01-01

190

The potential of matrix-assisted laser desorption/ionization mass spectrometry in the quality control of water buffalo mozzarella cheese.  

PubMed

Adulteration by addition of bovine milk to water buffalo milk employed for mozzarella cheese production is often observed. Water buffalo milk and mozzarella cheese were analysed by matrix-assisted laser desorption/ionization mass spectrometry in order to achieve their rapid and accurate characterization and to evaluate possible fraudulence in mozzarella cheese production. PMID:9654754

Angeletti, R; Gioacchini, A M; Seraglia, R; Piro, R; Traldi, P

1998-06-01

191

Brazilian isolates of Trypanosoma ( Megatrypanum) theileri: diagnosis and differentiation of isolates from cattle and water buffalo based on biological characteristics and randomly amplified DNA sequences  

Microsoft Academic Search

We detected and cultivated isolates of Trypanosoma (Megatrypanum) theileri from cattle and water buffaloes in São Paulo state, southeastern Brazil, which were characterized by comparing morphological, growth and molecular features. Although isolates from cattle and water buffalo were morphologically indistinguishable, they differed in their growth characteristics. A dendrogram based on randomly amplified polymorphic DNA (RAPD) patterns indicated close-genetic relationships among

A. C Rodrigues; M Campaner; C. S. A Takata; A Dell’ Porto; R. V Milder; G. F Takeda; M. M. G Teixeira

2003-01-01

192

Diversity of lactic Acid bacteria isolated from brazilian water buffalo mozzarella cheese.  

PubMed

The water buffalo mozzarella cheese is a typical Italian cheese which has been introduced in the thriving Brazilian market in the last 10 y, with good acceptance by its consumers. Lactic acid bacteria (LAB) play an important role in the technological and sensory quality of mozzarella cheese. In this study, the aim was to evaluate the diversity of the autochthones viable LAB isolated from water buffalo mozzarella cheese under storage. Samples were collected in 3 independent trials in a dairy industry located in the southeast region of Brazil, on the 28th day of storage, at 4 ºC. The LAB were characterized by Gram staining, catalase test, capacity to assimilate citrate, and production of CO2 from glucose. The diversity of LAB was evaluated by RAPD-PCR (randomly amplified polymorphic DNA-polymerase chain reaction), 16S rRNA gene sequencing, and by Vitek 2 system. Twenty LAB strains were isolated and clustered into 12 different clusters, and identified as Streptococcus thermophilus, Enterococcus faecium, Enterococcus durans, Leuconostoc mesenteroides subsp. mesenteroides, Lactobacillus fermentum, Lactobacillus casei, Lactobacillus delbrueckii subsp. bulgaricus, and Lactobacillus helveticus. Enterococcus species were dominant and citrate-positive. Only the strains of L. mesenteroides subsp. mesenteroides and L. fermentum produced CO2 from glucose and were citrate-positive, while L. casei was only citrate positive. This is the first report which elucidates the LAB diversity involved in Brazilian water buffalo mozzarella cheese. Furthermore, the results show that despite the absence of natural whey cultures as starters in production, the LAB species identified are the ones typically found in mozzarella cheese. PMID:25597646

Silva, Luana Faria; Casella, Tiago; Gomes, Elisangela Soares; Nogueira, Mara Correa Lelles; De Dea Lindner, Juliano; Penna, Ana Lúcia Barretto

2015-02-01

193

Helmintic infections in water buffaloes on Italian farms: a spatial analysis  

E-print Network

Abstract. The present paper reports the results of a cross-sectional survey aimed at obtaining up-to-date information on the spatial distribution of different groups and/or species of helminths in water buffaloes from central Italy. Geographical information systems (GIS) and spatial analysis were used to plan the sampling procedures, to display the results as maps and to detect spatial clusters of helminths in the study area. The survey was conducted on 127 water buffalo farms, which were selected in the study area using a grid sampling approach, followed by proportional allocation. Faecal samples (n. = 1,883) collected from the 127 farms were examined using the Flotac dual technique. Gastrointestinal strongyles were the most frequent helminths (33.1%) on the tested farms, followed by the liver fluke Fasciola hepatica (7.1%), the rumen fluke Calicophoron daubneyi (7.1%), the nematode Strongyloides spp. (3.1%), the lancet liver fluke Dicrocoelium dendriticum (2.4%) and the tapeworm Moniezia spp. (2.4%). In order to display the spatial distribution of the various helminths detected on the water buffalo farms (used as epidemiological unit in our study), point maps were drawn within the GIS. In addition, for each helminth, clustering of test-positive farms were investigated based on location determined by exact coordinates. Using spatial scan statistic, spatial clusters were found for the flukes F. hepatica and C. daubneyi and the cestode Moniezia spp.; these findings are consistent with the life cycle of these parasites, which have strong environmental determinants. In conclusion, the present study demonstrated that, with the appropriate survey-based data at hand, GIS is a useful tool to study epidemiological patterns of infections in

Laura Rinaldi; Vincenzo Musella; Vincenzo Veneziano; Renato U. Condoleo

194

Peripheral blood mononuclear cells: a potential cellular system to understand differential heat shock response across native cattle (Bos indicus), exotic cattle (Bos taurus), and riverine buffaloes (Bubalus bubalis) of India.  

PubMed

Circulating leukocytes can be used as an effective model to understand the heat stress response of different cattle types and buffaloes. This investigation aimed to determine the temporal profile of HSPs (HSP40, HSP60, HSP70, and HSP90) expression in circulating peripheral blood mononuclear cells (PBMCs) of Murrah buffaloes, Holstein-Friesian (HF), and Sahiwal cows in response to sublethal heat shock at 42 °C. The viability data indicated HF PBMCs to be the most affected to the heat shock, whereas Sahiwal PBMCs were least affected, indicating its better survivability during the heat stress condition. The qRT-PCR expression data showed significant increase in mRNA expression of the analyzed HSPs genes after heat stimuli to the PBMCs under in vitro condition. In each case, the HSPs were most upregulated at 2 h after the heat stress. Among the HSPs, HSP70 was relatively more expressed followed by HSP60 indicating the action of molecular chaperones to stabilize the native conformation of proteins. However, PBMCs from different cattle types and buffaloes showed difference in the extent of transcriptional response. The level of expression of HSPs throughout the time period of heat stress was highest in buffaloes, followed by HF and Sahiwal cows. The higher abundance of HSP70 mRNA at each time point after heat stress showed prolonged effect of heat stress in HF PBMCs. The data presented here provided initial evidence of transcriptional differences in PBMCs of different cattle types and buffaloes and warrant further research. PMID:24363171

Kishore, Amit; Sodhi, Monika; Kumari, Parvesh; Mohanty, A K; Sadana, D K; Kapila, Neha; Khate, K; Shandilya, Umesh; Kataria, R S; Mukesh, M

2014-09-01

195

Combining Aboriginal and Non-Aboriginal Knowledge to Assess and Manage Feral Water Buffalo Impacts on Perennial Freshwater Springs of the Aboriginal-Owned Arnhem Plateau, Australia  

NASA Astrophysics Data System (ADS)

Aboriginal land managers have observed that feral Asian water buffalo ( Bubalis bubalis Lydekker) are threatening the ecological and cultural integrity of perennial freshwater sources in Arnhem Land, Australia. Here we present collaborative research between the Aboriginal Rangers from Warddeken Land Management Limited and Western scientists which quantified the ground-level impacts of buffalo on seven perennial freshwater springs of the Arnhem Plateau. A secondary aim was to build the capacity of Aboriginal Rangers to self-monitor and evaluate the ecological outcomes of their land management activities. Sites with high buffalo abundance had significantly different ground, ground cover, and water quality attributes compared to sites with low buffalo abundance. The low buffalo abundance sites were characterized by tall herbaceous vegetation and flat ground, whereas wallows, bare ground, and short ungrazed grasses were indicators of sites with high buffalo abundance. Water turbidity was greater when buffalo abundance was high. The newly acquired monitoring skills and derived indicators of buffalo damage will be used by Aboriginal Rangers to assess the ecological outcomes of their future buffalo control efforts on the Arnhem Plateau.

Ens, Emilie-Jane; Cooke, Peter; Nadjamerrek, Ray; Namundja, Seraine; Garlngarr, Victor; Yibarbuk, Dean

2010-04-01

196

A signature protein-based method to distinguish Mediterranean water buffalo and foreign breed milk.  

PubMed

A novel genetic variant at the ?s1-casein locus of water buffalo (WB), 8-residue shorter than its wild-type has been found and sequenced. The internal deletion of the peptide E(35)KVNELsT(42) was confirmed by the isolation of the junction peptide. The 8-residue deletion mutant has a molecular weight that is 919 Da less than that of the wild-type. The novel isoform with a unique f35-42 deletion could be the result of the skipping of exon 6, generating an exon 6-deleted variant of ?s1-casein. The wild-type and its shortened ?s1-casein forms were found to co-exist in many individual milk samples. In contrast, the 8-residue, internally deleted ?s1-casein variant did not occur in water buffaloes of the Mediterranean breed reared in Italy. Wild-type ?s1-casein has 6 to 8 phosphate groups (P) while the internally deleted form 6 and 7P per molecule. PMID:23768399

Caira, Simonetta; Pinto, Gabriella; Balteanu, Valentin A; Chianese, Lina; Addeo, Francesco

2013-11-01

197

Stream habitat and water-quality information for sites in the Buffalo River Basin and nearby basins of Arkansas, 2001-2002  

USGS Publications Warehouse

The Buffalo River lies in north-central Arkansas and is a tributary of the White River. Stream-habitat and water-quality information are presented for 52 sites in the Buffalo River Basin and adjacent areas of the White River Basin. The information was collected during the summers of 2001 and 2002 to supplement fish community sampling during the same time period.

Petersen, James C.

2004-01-01

198

A comparative evaluation of parasitological tests and a PCR for Trypanosoma evansi diagnosis in experimentally infected water buffaloes  

Microsoft Academic Search

In this study five parasitological methods and a polymerase chain reaction (PCR) were compared for the diagnostic sensitivity for Trypanosoma evansi in experimentally infected water buffaloes over a period of 15 weeks. The combined estimates of sensitivity (CEse) of the PCR proved to be highest at 78.2%, closely followed by the mouse inoculation (MI), the micro-haematocrite centrifugation technique (MHCT) and

W. G Holland; F Claes; L. N My; N. G Thanh; P. T Tam; D Verloo; P Büscher; B Goddeeris; J Vercruysse

2001-01-01

199

Recombinant paramyosin (rec-Sj-97) tested for immunogenicity and vaccine efficacy against Schistosoma japonicum in mice and water buffaloes  

Microsoft Academic Search

A primary vaccine candidate antigen against schistosomiasis is paramyosin (pmy), a myofibrillar protein found exclusively in invertebrates. Here we report the results of vaccine trials against the Asian schistosome undertaken on inbred and outbred mice and water buffaloes using a bacterially expressed and purified form of Schistosoma japonicum pmy (rec-Sj-97). Vaccination of the mice resulted in high levels of specific

Donald P. McManus; Joanna Y. M. Wong; Jinchun Zhou; Chun Cai; Qingren Zeng; Danielle Smyth; Yuesheng Li; Bernd H. Kalinna; Mary J. Duke; Xinyuan Yi

2001-01-01

200

Prevalence of antibodies to Neospora caninum and Toxoplasma gondii in cattle and water buffaloes in southern Vietnam  

Microsoft Academic Search

Serum samples from 200 dairy cattle and 200 beef water buffaloes were collected in southern Vietnam during May to September 1995. The sera were analysed for antibodies to Neospora caninum by an enzyme-linked immunosorbent assay and the indirect fluorescent antibody test, and for antibodies to Toxoplasma gondii by the direct agglutination test. Significant levels of N. caninum antibodies were detected

L. T. T Huong; B.-L Ljungström; A Uggla; C Björkman

1998-01-01

201

Georeferencing livestock farms as tool for studying cystic echinococcosis epidemiology in cattle and water buffaloes from southern Italy  

Microsoft Academic Search

Cystic echinococcosis (CE), caused by the larval stages of the tapeworm Echinococcus granulosus, is known to be one of the most important parasitic infection in livestock worldwide and one of the most widespread zoonoses known. In the present study, we used a geographical information system (GIS) to study the spatial structure of livestock (cattle, water buffaloes and sheep) populations to

Giuseppe Cringoli; Laura Rinaldi; Vincenzo Musella; Vincenzo Veneziano; Maria Paola Maurelli; Francesco Di Pietro; Michele Frisiello; Salvatore Di Pietro

2007-01-01

202

Survival of Arcobacter butzleri during production and storage of artisan water buffalo mozzarella cheese.  

PubMed

Water buffalo mozzarella cheese (WBMC) is a fresh stretched cheese produced from whole chilled buffalo milk. Although pasteurization of milk and the use of defined starter cultures are recommended, traditional technology involving unpasteurized milk and natural whey cultures is still employed for WBMC production in Italy. The purpose of this study was to assess the behavior of Arcobacter butzleri during WBMC production and storage under different temperature conditions (5, 10, and 20 °C). Raw milk was experimentally inoculated with one reference strain and two isolates of A. butzleri, and the count was monitored during WBMC production and storage. The bacterial count of A. butzleri decreased during curd ripening (from 7.83 log colony-forming units (CFU)/g to 4.14 log CFU/g in about 4 h) and a further decrease (>4 log CFU/g) was observed at the end of curd stretching. During storage testing, A. butzleri was never detected by direct plating, whereas it was recovered from 12 of the total 162 WBMC until the end of storage testing by enrichment. The results revealed that A. butzleri is able to survive during WBMC production and storage at different temperature conditions. Consequently, traditional WBMC produced from raw milk could represent a potential source of Arcobacter infection for humans. PMID:23806147

Serraino, Andrea; Giacometti, Federica; Daminelli, Paolo; Losio, Marina N; Finazzi, Guido; Marchetti, Giacomo; Zambrini, Angelo V; Rosmini, Roberto

2013-09-01

203

Occurrence and molecular characterization of Cryptosporidium spp. and Enterocytozoon bieneusi in dairy cattle, beef cattle and water buffaloes in China.  

PubMed

Cryptosporidium spp. and Enterocytozoon bieneusi are important protists in a wide range of vertebrate hosts, causing diarrheal diseases. Cattle are considered potential reservoirs of Cryptosporidium infection in humans, although their role in the transmission of E. bieneusi is not clear. In the present work, 793 fecal specimens from dairy cattle, native beef cattle, and water buffaloes on 11 farms in China were examined for the presence of Cryptosporidium spp. and E. bieneusi using nested PCR targeting the small subunit (SSU) rRNA gene of Cryptosporidium spp. and the internal transcribed spacer (ITS) of E. bieneusi. For Cryptosporidium, 144/446 (32.3%) dairy cattle, 44/166 (26.5%) beef cattle, and 43/181 (23.8%) water buffaloes were PCR-positive. Sequence analysis was successful for 213 of the 231 Cryptosporidium-positive isolates; among them 94 had Cryptosporidium andersoni, 61 had Cryptosporidium bovis, 54 had Cryptosporidium ryanae, 2 had a Cryptosporidium suis-like genotype, and 2 had mixed infections of C. bovis and C. ryanae. In dairy and beef cattle, C. andersoni and C. bovis were the most common species, whereas C. ryanae was the dominant species in water buffaloes. The latter species produced SSU rRNA sequences different between cattle and water buffaloes. For E. bieneusi, the infection rate of E. bieneusi in dairy cattle, beef cattle and water buffaloes was 4.9%, 5.4% and 2.2%, respectively. All 35 E. bieneusi-positive specimens were successfully sequenced, revealing the presence of four genotypes: three Group 2 genotypes previously reported in cattle as well as humans (I, J and BEB4) and one Group 1 genotype recently reported in yaks (CHN11). Genotypes I and J were the most common genotypes in dairy and beef cattle, while genotype CHN11 was the only genotype seen in water buffaloes. Thus, the distribution of Cryptosporidium spp. and E. bieneusi in water buffaloes might be different from in dairy and beef cattle in China. These findings indicate that some of the Cryptosporidium species and all four E. bieneusi genotypes identified in bovine animals in the study areas may have zoonotic potential. PMID:25541482

Ma, Jingbo; Li, Pei; Zhao, Xiaoping; Xu, Hailing; Wu, Wenxian; Wang, Yuanfei; Guo, Yaqiong; Wang, Lin; Feng, Yaoyu; Xiao, Lihua

2015-01-30

204

33 CFR 117.773 - Buffalo River.  

Code of Federal Regulations, 2013 CFR

...Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Buffalo River. 117.773 Section...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...New York § 117.773 Buffalo River. (a) The...

2013-07-01

205

33 CFR 117.773 - Buffalo River.  

Code of Federal Regulations, 2011 CFR

...Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Buffalo River. 117.773 Section...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...New York § 117.773 Buffalo River. (a) The...

2011-07-01

206

33 CFR 117.773 - Buffalo River.  

...Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Buffalo River. 117.773 Section...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...New York § 117.773 Buffalo River. (a) The...

2014-07-01

207

33 CFR 117.773 - Buffalo River.  

Code of Federal Regulations, 2010 CFR

...Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Buffalo River. 117.773 Section...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...New York § 117.773 Buffalo River. (a) The...

2010-07-01

208

33 CFR 117.773 - Buffalo River.  

Code of Federal Regulations, 2012 CFR

...Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Buffalo River. 117.773 Section...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...New York § 117.773 Buffalo River. (a) The...

2012-07-01

209

Retention of Anchor and Spaghetti Tags by Paddlefish, Catfishes, and Buffalo Fishes  

Microsoft Academic Search

Tag retention was measured for paddlefish Polyodon spathula, channel catfish Ictalurus punctatus, blue catfish Ictalurus furcatus, smallmouth buffalo Ictiobus bubalus, and bigmouth buffalo Ictiobus cyprinellus. Fish were captured in 1986 and 1987, triple-tagged with spaghetti tags (Floy FT-4 lock-on), anchor tags (Floy FD-6813), and fin or opercular marks, and released into Energy Lake, Kentucky. After testing for homogeneity, we constructed

Tom J. Timmons; Mark H. Howell

1995-01-01

210

Effect of timing of development on total cell number and expression profile of HSP-70.1 and GLUT-1 in buffalo (Bubalus bubalis) oocytes and preimplantation embryos produced in vitro.  

PubMed

The present study was designed to compare the expression profile of two developmentally important genes (HSP-70.1 and GLUT-1) and TCN (total cell number) count in fast (group A) and slow (group B) cleaved buffalo embryos to access their in vitro developmental competence. Buffalo COCs (cumulus oocyte complexes) were collected from local abattoir ovaries and subjected to in vitro maturation in: TCM-199 supplemented with 10% FBS (fetal bovine serum), BSA (3 mg/ml), sodium pyruvate (0.25 mM) and 20 ng/ml EGF (epidermal growth factor) at 38.5 degrees C under 5% CO2. In vitro derived embryos were collected at 4-8, 8-16 cell, morula and blastocyst stages at specific time points for gene expression analysis and total cell count. A semiquantitative RT-PCR (reverse transcriptase-PCR) assay was used to determine the HSP-70.1 and GLUT-1 transcripts. Results showed that developmental competence and TCN count in fast (group A)-cleaving embryos was significantly (P<0.05) higher than in the slow group (group B). The gene transcript of HSP-70.1 and GLUT-1 was expressed in oocytes (immature and mature) and throughout the embryonic developmental stages in the fast group (group A), while in the slow (group B) cleaving embryos, the expression of HSP-70.1 was absent in all the embryonic developmental stages, and expression of GLUT-1 was absent after 8-16 cell stage. In conclusion, TCN count and expression profile of HSP-70.1 and GLUT-1 genes in buffalo embryos are different taking into account the cleavage rate. Quality of such embryos for research purposes, TCN and expression profiling of developmentally important genes should be employed to optimize the in vitro culture system to produce superior quality of embryos. PMID:20100172

Rajhans, Rajib; Kumar, G Sai; Dubey, Pawan K; Sharma, G Taru

2010-05-01

211

Expression of mRNA encoding IGF-I, IGF-II, type-I,and II IGF-receptors and IGF-binding proteins-1-4 during ovarian follicular development in buffalo (Bubalus bubalis).  

PubMed

The present study was designed to investigate the expression pattern of IGF-I, IGF-II, type-I and II IGF-receptors, and IGFBP-1-4 in different stages of buffalo ovarian preantral follicles (PFs), antral follicles (AFs), ovulatory follicles (OFs), and immature (IM) and in vitro matured (MO) oocytes. Buffalo ovaries were collected from local abattoir, PFs (200-250 µm), AFs (1-3 mm), and OFs (5-8 mm) were isolated by mechanical method. PFs, AFs, OFs, and oocytes were lysed to release mRNA, reverse transcribed, and then subjected to RT-PCR, whereas protein were localized through immunohistochemistry. Relative expression of mRNA transcripts was clearly seen for IGF-II, type-I and II IGF-receptors, and IGFBP-1-4 in all the stages of developing follicles and oocytes. We were unable to detect mRNA and protein expression of IGF-1 in any of the oocytes or follicles at any stage of the development. IGF-II and both IGF receptors mRNA expression were found higher (P < 0.05) in PFs compared to AFs and OFs. Expression of IGFBP-1 and 2 in PFs, as well as IGFBP-3 and 4 in AFs, was found with higher (P < 0.05) levels. The expression results were further confirmed by localization of IGF-II, type-I and II IGF-receptors, and IGFBP-1-4 proteins. In conclusion, IGF-II appears to be the only ligand that is endogenously expressed by all the follicular stages and oocytes, which may act in an autocrine manner through the Type-1 IGF receptor. Expression of IGFBP-1-4 and IGF-II suggests the possible role of these genes in recruitment, growth, proliferation, and steroidogenic responses during developmental phases of buffalo ovarian follicles. PMID:25380459

Dubey, Pawan K; Nath, Amar; Chandra, Vikash; Sarkar, Mihir; Saikumar, G; Sharma, G Taru

2015-01-01

212

Follicular dynamics in water buffalo superovulated in presence or absence of a dominant follicle  

Microsoft Academic Search

The ovaries of 12 buffalo were examined daily by ultrasound beginning at Day 3 of the estrous cycle, followed by superovulation between Days 10 and 13 of the cycle. The buffalo were divided into 2 groups on the basis of the presence (dominant, n = 7) or absence (nondominant, n = 5) of a dominant follicle at the start of

M. Taneja; G. Singh; S. M. Totey; A. Ali

1995-01-01

213

Effect on quarter milk somatic cell count and antimicrobial susceptibility of Staphylococcus rostri causing intramammary infection in dairy water buffaloes.  

PubMed

In many parts of the world, coagulase-negative staphylococci (CNS) are the predominant cause of intramammary infections (IMI) in dairy cows and in water buffaloes, as well. A longitudinal field study was carried out on one well-managed dairy water buffalo herd to determine the prevalence and distribution of CNS and a recently described CNS-species, Staphylococcus rostri, in milk samples to explore its relevance for buffaloes' udder health throughout lactation, and to gain insight into the susceptibility of the latter species toward commonly used antimicrobials. Twice weekly quarter milk samples from a cohort of 11 lactating water buffaloes were collected over an 8-mo period. The CNS (n=109; 76.2% of all culture-positive samples) were the predominant pathogens causing IMI, followed by Corynebacterium bovis (n=11; 7.6%) and Streptococcus spp. (n=9; 6.2%) other than Stretococcus uberis (n=2; 1.4%). Thirty-seven hemolytic staphylococci suspected to be Staphylococcus aureus were further differentiated using transfer DNA-intergenic spacer-PCR and rpoB-gene sequencing because they were coagulase-negative. Thirty-three of those isolates were identified as Staph. rostri, whereas 2 others were identified as Staphylococcus epidermidis. None of the Staph. rostri isolates displayed resistance to the antimicrobial agents tested. Mean quarter milk somatic cell count (qSCC) of all samples collected throughout lactation was 20,970 cells/mL. The qSCC at sampling of quarters infected with Staph. rostri (34,466 cells/mL) and CNS other than Staph. rostri (34,813 cells/mL) were significantly higher than the qSCC of noninfected quarters (20,287 cells/mL), yet not significantly different from each other. These findings provide novel insight into the prevalence and distribution, antimicrobial susceptibility, and relevance of Staph. rostri compared with other CNS species causing IMI in water buffaloes. Further studies are needed to pinpoint the relevance, niches, and transmission routes of Staph. rostri, as well as other CNS in water buffaloes. PMID:23548306

Locatelli, C; Piepers, S; De Vliegher, S; Barberio, A; Supré, K; Scaccabarozzi, L; Pisoni, G; Bronzo, V; Haesebrouck, F; Moroni, P

2013-06-01

214

Phylogenetic analysis and comparison between cow and buffalo (including Egyptian buffaloes) mitochondrial displacement-loop regions.  

PubMed

Mitochondrial DNA (mtDNA) analysis has been used extensively for phylogenetic analysis studies and systematics. The displacement loop (D-loop) region inside the mtDNA is a non-coding part whose analysis can indicate variations between closely related populations. This paper reports for the first time the characterization and analysis of the complete sequence of the D-loop region from Egyptian buffaloes and analysis in conjunction with previously published Indian and European Bubalus bubalis and Bos sub-tribe sequences. In the entire D-loop of the Egyptian buffaloes, we identified four haplotypes and nine polymorphic sites from the nine sequenced D-loop regions--while in the studied set of buffaloes we identified 28 polymorphic sites in the entire D-loop, and 49 polymorphic sites in the case of cows. Alignment between buffaloes and cows to evaluate the characteristics of the D-loop region showed that the second region of the conserved sequence block (CSB2) is apparently the most variable region in the D-loop between cows and buffaloes, with four insertions in all buffaloes and two substitutions, followed by the second region of the extended termination associated sequence (ETAS2) with a substitution rate of 1/10. The Egyptian buffaloes were shown to be closest to the Italian counterparts, exemplifying the closeness of ethnicity and the history of civilization of that region. PMID:19462514

Ramadan, Hassan A I; El-Hefnawi, Mahmoud M

2008-08-01

215

Identification of adulteration in water buffalo mozzarella and in ewe cheese by using whey proteins as biomarkers and matrix-assisted laser desorption/ionization mass spectrometry.  

PubMed

A rapid and accurate method to identify bovine and ewe milk adulteration of fresh water buffalo mozzarella cheese by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is described. The differentiation among mozzarella made from water buffalo milk and from mixtures of less expensive bovine and, more recently, ewe milk with water buffalo milk is achieved using whey proteins, alpha-lactalbumin and beta-lactoglobulins as molecular markers. It is worth noting that the method proposed here is, to our knowledge, the first strategy able to characterize possible fraudulent additions of ewe milk in samples of water buffalo milk devoted to the production of water buffalo mozzarella cheese. In addition, a linear relationship was found between the relative response of the molecular ion and the abundance of the analysed whey proteins. This demonstrates that this approach can be used to determine the amount of bovine or ovine milk added to water buffalo milk employed for mozzarella cheese production. Furthermore, this method also appears suitable for the analysis of ewe cheese. Hence these findings open the way to a new field for mass spectrometry in the evaluation of possible fraudulence in dairy industry production. PMID:12271441

Cozzolino, R; Passalacqua, S; Salemi, S; Garozzo, D

2002-09-01

216

Incidence of Listeria species in bovine, ovine, caprine, camel and water buffalo milk using cultural method and the PCR assay  

PubMed Central

Objective To determine the prevalence rate of Listeria species in bovine, ovine, caprine, camel and water buffalo milk in Iran. Methods From September 2010 to December 2011 a total of 260 bulk milk samples including 85 bovine, 37 camel, 34 water buffalo, 56 ovine and 48 caprine bulk milk samples were collected from commercial dairy herds, in Fars and Khuzestan provinces, Iran and were evaluated for the presence of Listeria species using cultural method and the PCR assay. Results Using cultural method, 19 samples (7.3%) were positive for Listeria spp. The highest prevalence of Listeria was found in raw water buffalo milk (11.8%), followed by raw bovine milk (10.6%), raw ovine milk (7.1%), and raw caprine milk (4.2%) samples. All 37 camel milk samples from 20 camel breeding farms were negative for Listeria spp. The overall prevalence of Listeria was 7.3%, in which Listeria innocua was the most recovered species (4.2%); the remaining isolates were Listeria monocytogenes (1.9%), Listeria ivanovii (0.08%) and Listeria seeligari (0.04%). The PCR assay could identify 8 Listeria-contaminated milk samples that were negative using the cultural method. Conclusions The results presented in this study indicate the potential risk of infection with Listeria in people consuming raw and unpasteurized milk.

Rahimi, Ebrahim; Momtaz, Hassan; Behzadnia, Asma; Baghbadorani, Zeinab Torki

2014-01-01

217

Detection of antibodies against Brucella abortus, Leptospira spp., and Apicomplexa protozoa in water buffaloes in the Northeast of Argentina.  

PubMed

Water buffalo industry has become a profitable activity worldwide, including the Northeast of Argentina (NEA). However, research on diseases affecting this species is scarce. The aim of the present study was to detect antibodies against Brucella abortus, Leptospira spp., Neospora caninum, Toxoplasma gondii, and Sarcocystis spp. in 500 water buffalo cows from five ranches (100 animals each) in the NEA. Serum samples were tested for B. abortus by fluorescence polarization assay, Leptospira spp. by microagglutination test, and N. caninum, T. gondii, and Sarcocystis spp. by indirect fluorescent antibody tests. Overall, the proportion of seropositive animals was 6.4, 22.2, 42.2, 25.4, and 50.8 % for brucellosis, leptospirosis, neosporosis, toxoplasmosis, and sarcocystosis, respectively. The proportion of seropositive animals for all diseases was statistically different among herds (p?water buffaloes in the NEA is reported in this study. PMID:23765549

Konrad, José L; Campero, Lucía M; Caspe, Gastón S; Brihuega, Bibiana; Draghi, Graciela; Moore, Dadin P; Crudeli, Gustavo A; Venturini, María C; Campero, Carlos M

2013-11-01

218

Hydrologic and water-quality characteristics for Bear Creek near Silver Hill, Arkansas, and selected Buffalo River sites, 1999-2004  

USGS Publications Warehouse

The purpose of this report is to describe and compare the hydrologic and water-quality characteristics of Bear Creek near Silver Hill, Arkansas, to two sites on the Buffalo River upstream from the confluence of Bear Creek, to a site on Calf Creek, a smaller tributary to the Buffalo River, to selected undeveloped sites across the Nation, and to a developed site in Arkansas. A better understanding of the hydrology and water quality of Bear Creek is of interest to many, including the National Park Service, which administers the Buffalo National River, to evaluate its effects on the hydrology and water quality of the Buffalo River. The streamflow at Bear Creek near Silver Hill varied seasonally and annually from January 1999 to March 2004. The mean annual streamflow at Bear Creek for calendar years 1999 to 2003 was 86.0 cubic feet per second. The highest annual mean streamflow occurred in 2002 (158 cubic feet per second) and the lowest annual mean streamflow occurred in 1999 (56.4 cubic feet per second). The mean annual streamflow for calendar years 1999 to 2003 at the Buffalo River near Boxley and Buffalo River near St. Joe was 102 and 881 cubic feet per second, respectively. Concentrations of nitrogen measured for Bear Creek generally were greater than concentrations measured at the two Buffalo River sites and were similar to concentrations measured at Calf Creek. Concentrations of phosphorus measured at Bear Creek generally were greater than concentrations measured at the two Buffalo River sites and were similar to concentrations measured at Calf Creek. Fecal indicator bacteria concentrations generally were greater at Bear Creek than concentrations measured at the Buffalo River and similar to concentrations at Calf Creek. Bear Creek had significantly greater suspended-sediment concentrations than the Buffalo River near Boxley and the Buffalo River near St. Joe and similar concentrations to Calf Creek. Nutrients, dissolved organic carbon, and suspended-sediment loads at Bear Creek and two Buffalo River sites varied because of differences in land use and contributing drainage area for each site. In general, the Buffalo River near St. Joe had the greatest annual loads of nutrients, dissolved organic carbon, and suspended sediment. The Buffalo River near Boxley had the least annual nutrient and suspended-sediment loads amongthe three sites. Buffalo River near Boxley had lesser annual loads than the other two sites probably because of the higher percentage of forested land in the basin and smaller contributing drainage area. Mean annual nutrient, dissolved organic carbon, and suspended- sediment yields computed for Bear Creek were greater than yields computed for both of the Buffalo River sites. Bear Creek had greater median annual nutrient yields than selected undeveloped basins across the Nation and less median annual nutrient yields than the Illinois River south of Siloam Springs, Arkansas, which is representative of a developed basin. Bear Creek had greater median annual flow-weighted nutrient concentrations than the Buffalo River near St. Joe, the Buffalo River near Boxley, and selected undeveloped sites across the Nation. Bear Creek had less median flow-weighted nutrient concentrations than the Illinois River.

Galloway, Joel M.; Green, W. Reed

2004-01-01

219

Diagnostic value of the recombinant tandem repeat antigen TeGM6-4r for surra in water buffaloes.  

PubMed

Trypanosoma evansi infection, or surra, is currently affecting various species of animals, especially water buffaloes. Since diagnosis is an important aspect of surra control, development of novel diagnostic antigens is of interest to implement and improve the currently utilized methods. Our study evaluated the tandem repeat antigen TeGM6-4r in T. evansi antibody detection in water buffaloes. TeGM6-4r-based ELISA was performed with 20 positive and 8 negative controls and 484 field samples from water buffaloes in Northern Vietnam. To examine cross-reactivity, sera from Japanese cattle that had been experimentally infected with Theileria orientalis (n=10), Babesia bovis (n=3), Babesia bigemina (n=7) and Trypanosoma theileri (n=59) were included in the study. The sensitivity of the test was 80%. TeGM6-4r did not react with Theileria or Babesia infected sera, however it showed cross reactivity with 11/59 T. theileri infected samples. The reference test, CATT/T. evansi also reacted with 3/59 T. theileri infected sera. The lysate antigen-based ELISA reacted with 4/59 T. theileri, 9/10 Theileria and 3/10 Babesia infected sera. In contrast, TeGM6-4r-based ELISA was 86.3% sensitive and 58.3% specific in the screening of field samples. The average seroprevalence of T. evansi infection among water buffaloes in Northern Vietnam was 27.1% by CATT/T. evansi and 53.7% by TeGM6-4r. Seroprevalence in the five surveyed provinces ranged from 17.4% to 39.8% in the reference test, and 47.3% to 67.3% in the recombinant antigen based test. The finding indicated that the disease is still widely endemic in the area and that surveillance programs need to be carried out regularly to better control surra. We proposed TeGM6-4r as a useful serodiagnostic antigen for the detection and epidemiological surveillance of T. evansi infection among water buffaloes. PMID:24524896

Nguyen, Thu-Thuy; Zhou, Mo; Ruttayaporn, Ngasaman; Nguyen, Quoc Doanh; Nguyen, Viet Khong; Goto, Yasuyuki; Suzuki, Yasuhiko; Kawazu, Shin-ichiro; Inoue, Noboru

2014-03-17

220

Genetic Diversity in the Prion Protein Gene (PRNP) of Domestic Cattle and Water Buffaloes in Vietnam, Indonesia and Thailand  

PubMed Central

ABSTRACT There has been an accumulation of information on frequencies of insertion/deletion (indel) polymorphisms within the bovine prion protein gene (PRNP) and on the number of octapeptide repeats and single nucleotide polymorphisms (SNPs) in the coding region of bovine PRNP related to bovine spongiform encephalopathy (BSE) susceptibility. We investigated the frequencies of 23-bp indel polymorphism in the promoter region (23indel) and 12-bp indel polymorphism in intron 1 region (12indel), octapeptide repeat polymorphisms and SNPs in the bovine PRNP of cattle and water buffaloes in Vietnam, Indonesia and Thailand. The frequency of the deletion allele in the 23indel site was significantly low in cattle of Indonesia and Thailand and water buffaloes. The deletion allele frequency in the 12indel site was significantly low in all of the cattle and buffaloes categorized in each subgroup. In both indel sites, the deletion allele has been reported to be associated with susceptibility to classical BSE. In some Indonesian local cattle breeds, the frequency of the allele with 5 octapeptide repeats was significantly high despite the fact that the allele with 6 octapeptide repeats has been reported to be most frequent in many breeds of cattle. Four SNPs observed in Indonesian local cattle have not been reported for domestic cattle. This study provided information on PRNP of livestock in these Southeast Asian countries. PMID:24705506

UCHIDA, Leo; HERIYANTO, Agus; THONGCHAI, Chalermchaikit; HANH, Tran Thi; HORIUCHI, Motohiro; ISHIHARA, Kanako; TAMURA, Yutaka; MURAMATSU, Yasukazu

2014-01-01

221

Genetic diversity in the prion protein gene (PRNP) of domestic cattle and water buffaloes in Vietnam, Indonesia and Thailand.  

PubMed

There has been an accumulation of information on frequencies of insertion/deletion (indel) polymorphisms within the bovine prion protein gene (PRNP) and on the number of octapeptide repeats and single nucleotide polymorphisms (SNPs) in the coding region of bovine PRNP related to bovine spongiform encephalopathy (BSE) susceptibility. We investigated the frequencies of 23-bp indel polymorphism in the promoter region (23indel) and 12-bp indel polymorphism in intron 1 region (12indel), octapeptide repeat polymorphisms and SNPs in the bovine PRNP of cattle and water buffaloes in Vietnam, Indonesia and Thailand. The frequency of the deletion allele in the 23indel site was significantly low in cattle of Indonesia and Thailand and water buffaloes. The deletion allele frequency in the 12indel site was significantly low in all of the cattle and buffaloes categorized in each subgroup. In both indel sites, the deletion allele has been reported to be associated with susceptibility to classical BSE. In some Indonesian local cattle breeds, the frequency of the allele with 5 octapeptide repeats was significantly high despite the fact that the allele with 6 octapeptide repeats has been reported to be most frequent in many breeds of cattle. Four SNPs observed in Indonesian local cattle have not been reported for domestic cattle. This study provided information on PRNP of livestock in these Southeast Asian countries. PMID:24705506

Uchida, Leo; Heriyanto, Agus; Thongchai, Chalermchaikit; Hanh, Tran Thi; Horiuchi, Motohiro; Ishihara, Kanako; Tamura, Yutaka; Muramatsu, Yasukazu

2014-07-01

222

The influence of T. evansi infection on the immuno-responsiveness of experimentally infected water buffaloes  

Microsoft Academic Search

In order to define the immuno-suppressive capacity of Trypanosoma evansi infections in buffaloes on the induction of immune responses against heterologous antigens, infected and non-infected buffaloes were vaccinated against Pasteurella multocida (haemorrhagic septicemia) and were simultaneously immunised with a control antigen, human serum albumin (HSA). Antibody responses against HSA were significantly reduced in T. evansi-infected animals, but no conclusive data

W. G. Holland; L. N. My; T. V. Dung; N. G. Thanh; P. T. Tam; J. Vercruysse; B. M. Goddeeris

2001-01-01

223

Lousicidal, ovicidal and repellent efficacy of some essential oils against lice and flies infesting water buffaloes in Egypt.  

PubMed

The lousicidal and repellent effects of five essential oils were investigated for the first time against the buffalo louse, Haematopinus tuberculatus, and flies infesting water buffaloes in Qalyubia Governorate, Egypt. For the in vitro studies, filter paper contact bioassays were used to test the oils and their lethal activities were compared with that of d-phenothrin. Four minutes post-treatment, the median lethal concentration, LC50, values were 2.74, 7.28, 12.35, 18.67 and 22.79% for camphor (Cinnamomum camphora), onion (Allium cepa), peppermint (Mentha piperita), chamomile (Matricaria chamomilla) and rosemary oils (Rosmarinus officinalis), respectively, whereas for d-phenothrin, it was 1.17%. The lethal time (50) (LT50) values were 0.89, 2.75, 15.39, 21.32, 11.60 and 1.94 min after treatment with 7.5% camphor, onion, peppermint, chamomile, rosemary and d-phenothrin, respectively. All the materials used except rosemary, which was not applied, were ovicidal to the eggs of H. tuberculatus. Despite the results of the in vitro assays, the in vivo treatments revealed that the pediculicidal activity was more pronounced with oils. All treated lice were killed after 0.5-2 min, whereas with d-phenothrin, 100% mortality was reached only after 120 min. The number of lice infesting buffaloes was significantly reduced 3, 6, 4, 6 and 9 days after treatment with camphor, peppermint, chamomile, onion, and d-phenothrin, respectively. Moreover, the oils and d-phenothrin significantly repelled flies, Musca domestica, Stomoxys calcitrans, Haematobia irritans and Hippobosca equina, for 6 and 3 days post-treatment, respectively. No adverse effects were noted on either animals or pour-on operators after exposure to the applied materials. Consequently, some Egyptian essential oils show potential for the development of new, speedy and safe lousicides and insect repellents for controlling lice and flies which infest water buffaloes. PMID:19596520

Khater, Hanem F; Ramadan, Mohamed Y; El-Madawy, Reham S

2009-10-14

224

Effect of flubendiamide, lead and their combined exposure on erythrocytic indices in water buffalo calves.  

PubMed

This study evaluated the effect of flubendiamide, lead and their combined oral administration on erythrocytic indices in water buffalo calves. Exposure to flubendiamide alone resulted in non-significant decreases in Hemoglobin (Hb), packed cell volume (PCV), total erythrocyte count (TEC), mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH). Lead acetate exposure resulted in significant declines in Hb, PCV and TEC. Combined exposure to flubendiamide and lead resulted in declines in Hb, TEC and PCV, but values did not differ significantly from corresponding values in the group treated with lead alone. Changes in MCV, MCHC and MCH were inconsistent. Plasma calcium concentration declined on day 90 in lead-exposed animals, but increased again on day 30 post-treatment. Results of the present study indicated that flubendiamide exposure results in marginal alterations in erythrocytic indices, but lead exposure caused significant declines in Hb, PCV and TEC. No interactive effects were observed for flubendiamide and lead on changes in erythrocytic indices. PMID:24292848

Ranjan, Amita; Dumka, Vinod Kumar; Singh, Nittin Dev

2014-04-01

225

Base flow, water quality, and streamflow gain and loss of the Buffalo River, Arkansas, and selected tributaries, July and August 2003  

USGS Publications Warehouse

A study of the Buffalo National River in north-central Arkansas was conducted between July 28-30 and August 13-15, 2003, to characterize the base-flow and water-quality characteristics and streamflow gain and loss in the Buffalo River. The study was separated into two time periods because of a precipitation event that occurred on the afternoon of July 30 causing appreciable storm runoff. Streamflow was separated to identify base-flow and surface-runoff components using the Base Flow Index hydrograph separation computer program. Base-flow separation analyses indicated annual variability in streamflow throughout the Buffalo River Basin. Based upon these analyses, total and base flow were below average for the mainstem of the river and Richland Creek during the 2003 water year. Waterquality samples were collected from 25 surface-water sites on the Buffalo River and selected tributaries. Most nutrient concentrations for the mainstem of the Buffalo River were near or below the minimum reporting level and were less than the median flow-weighted concentration for relatively undeveloped stream basins in the United States. Streamflow measurement data were collected at 44 locations along the mainstem of the Buffalo River and at points of inflow (prior to confluence with the mainstem) to identify gaining and losing reaches. Seven gaining and five losing reaches were identified for the Buffalo River. Additionally, surface flow on the mainstem of the Buffalo River was diverted to subsurface flow on the mainstem at two locations (river miles 73.6 and 131.6) where the mainstem was found to be dry. Reaches throughout the length of the river had calculated gains or losses that were less than the sum of measurement errors for the respective reaches of river.

Moix, Matthew W.; Galloway, Joel M.

2005-01-01

226

Differential expression dynamics of Growth differentiation factor9 (GDF9) and Bone morphogenetic factor15 (BMP15) mRNA transcripts during in vitro maturation of buffalo (Bubalus bubalis) cumulus-oocyte complexes.  

PubMed

The present study has evaluated the association of growth differentiation factor9 (GDF9) and bone morphogenetic protein15 (BMP15) mRNA expression in cumulus-oocyte complexes (COCs) of buffalo ovary during in vitro maturation (IVM). GDF9 and BMP15 are expressed specifically in mammalian oocytes and also participate in cumulus-oocyte crosstalk. Quantitative real-time polymerase chain reaction (qRT-PCR) technique was applied to investigate the relative abundance (RA) of GDF9 and BMP15 mRNA transcripts throughout the IVM process. Relative mRNA expression pattern of these specific genes were assessed in oocytes and cumulus cells at 0, 6, 12 and 24 h of in vitro culture. Our results revealed that RA of GDF9 during different hours of IVM showed significant reduction between 0 h and 24 h of maturation in oocytes and BMP15 transcript increased significantly (P<0.05) between 6 h and 12 h and decreased again between 12 h and 24. In cumulus cells, GDF9 remained stable during IVM upto 12 h of maturation and decreased significantly between 12 h and 24 h of maturation. Conversely, significant reduction of BMP15 was observed between 0 h and 6 h, stayed stable upto 12 h and became undetectable at 24 h of maturation. In conclusion, these two genes were differentially expressed during the period of oocyte maturation process and notably, BMP15 expression pattern is associated specifically with the period of cumulus cell expansion. PMID:23724366

Kathirvel, Muralidharan; Soundian, Eswari; Kumanan, Vijayarani

2013-12-01

227

Hydrologic and Water-Quality Characteristics for Calf Creek Near Silber Hill, Arkansas and Selected Buffalo River Sites, 2001-2002  

USGS Publications Warehouse

The Buffalo River and its tributary, Calf Creek, are in the White River Basin in the Ozark Plateaus physiographic province in north-central Arkansas. A better understanding of the hydrology and water quality of Calf Creek is of interest to many, including the National Park Service, which administers the Buffalo National River, to evaluate its effect on the hydrology and water quality of the Buffalo River. The streamflow and water-quality characteristics of Calf Creek near Silver Hill, Arkansas, were compared to two sites on the Buffalo River upstream (near Boxley, Arkansas) and downstream (near St. Joe, Arkansas) from the confluence of Calf Creek for calendar years 2001 and 2002. Annual and seasonal loads were estimated for Calf Creek for nutrients, dissolved organic carbon, and suspended sediment and compared with loads at sites on the Buffalo River. Flow-weighted concentrations and yields were computed from estimated annual loads for comparison with other developed and undeveloped basins. Streamflow varied annually and seasonally at the three sites. The Buffalo River near St. Joe had the largest annual mean streamflow (805 to 1,360 cubic feet per second for 2001 and 2002) compared to the Buffalo River near Boxley (106 and 152 cubic feet per second for 2001 and 2002) and Calf Creek (39 and 80 cubic feet per second for 2001 and 2002). Concentrations of nutrients, suspended sediment, and fecal indicator bacteria generally were greater in samples from Calf Creek than in samples collected from both Buffalo River sites. Bacteria and suspended-sediment concentrations were greater in samples collected during high-flow events at all three sites. The Buffalo River near Boxley had the lowest concentrations for nutrients, suspended sediment, and fecal indicator bacteria. Estimated annual loads of the nutrients, suspended sediment, and organic carbon for 2001 and 2002 demonstrated substantial variability between the three sites and through time. Estimated loads for nutrients at the Buffalo River near St. Joe were 7 to 27 times the median loads estimated for Calf Creek and suspended sediment loads were as much as 120 times greater. Dissolved organic carbon loads were 16 to 20 times greater at the Buffalo River near St. Joe than for Calf Creek. The Buffalo River near Boxley had the smallest annual loads for all constituents except for suspended sediment, which were slightly greater than suspended sediment loads estimated for Calf Creek. Higher loads would be expected at the Buffalo River near St. Joe because of the larger basin area and larger volume of streamflow. Likewise, estimated loads for all three sites were greater during seasons that had greater streamflow than during seasons with more frequent periods of base-flow conditions. The highest daily loads occurred in the fall and winter of 2001 and the winter and spring of 2002.Flow-weighted concentrations generally were higher for Calf Creek than concentrations for the two sites on the Buffalo River and for typical flow-weighted concentrations found in undeveloped basins. However, the flow-weighted concentrations were lower than concentrations in a developed basin. Annual yields calculated for Calf Creek were higher than the two sites on the Buffalo River and sites that are representative of undeveloped basins but lower than a site representative of a developed basin. The Buffalo River near Boxley had yields that were less than the yields typical of undeveloped basins.

Galloway, Joel M.; Green, W. Reed

2004-01-01

228

Yunsheng Xu e-mail: yunsheng@acsu.buffalo.edu  

E-print Network

Yunsheng Xu e-mail: yunsheng@acsu.buffalo.edu Xiangcheng Luo D. D. L. Chung e-mail: ddlchung@acsu.buffalo.edu Composite Materials Research Laboratory, State University of New York at Buffalo, Buffalo, NY 14260 as that given by solder. Water is almost as effective as sodium silicate without filler, but the thermal contact

Chung, Deborah D.L.

229

Comparative virulence of three Trypanosoma evansi isolates from water buffaloes in the Philippines.  

PubMed

The virulence of three Trypanosoma evansi isolates in Luzon, Visayas and Mindanao water buffaloes was compared determining the mortality rate, parasitemia level, clinical signs, and lesions on mice. A total of 51 inbred Balb/c mice (5-6 weeks old) were used and divided into two sets. Set A had three groups corresponding to three trypanosomes isolates (Luzon, Visayas, and Mindanao) with seven mice each whose parasitemia level, clinical signs, and lesions were noted at necropsy. Set B had three groups corresponding to the three isolates with ten mice each whose mortality was monitored. Each infected mouse was inoculated with 0.2 ml of T. evansi intraperitoneally and blood was examined under high power magnification. Their parasitemia level was determined using "Rapid Matching Method". Dead mice were subjected to necropsy and the lungs, liver, spleen, brain and heart were subjected to histopathological processing. Results showed that the mortality rate was highest at Day 3 for the Visayas isolates (70%), while at Day 5 for Luzon (90%) and Mindanao (70%) isolates. The parasitemia level of Visayas isolates (1×10(8.7)) reached the earliest peak at Day 4 while Luzon isolates (1×10(9)) at Day 6 and Mindanao isolates (1×10(8.7)) at Day 8. Statistical analysis using Least significant difference (LSD) revealed significant difference among treatment means at Days 2 and 4. All of the affected mice showed rough hair coat, decreased body weight, and decreased packed cell volume. The most obvious gross lesions observed were pale liver with petechiations and pale muscles. Histopathological examination revealed depletion of the red pulp and extramedullary hematopoiesis in the spleen. Congestion, intralesional trypanosomes in blood vessel and extramedullary hematopoiesis were observed in the liver. In the lungs non-specific lesions observed were pulmonary edema, congestion and hemosiderosis. PMID:22154978

Verdillo, John Christian M; Lazaro, Jonathan V; Abes, Nancy S; Mingala, Claro N

2012-02-01

230

Yeasts and hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in Minas Gerais, Brazil  

PubMed Central

The aim of this work was to study the yeast populations and the main hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in Minas Gerais, Brazil. Forty-two water buffalo mozzarella samples were purchased from retail outlets in Belo Horizonte. In addition, five samples of consecutive starter cultures, curd before acidification, acidified curd and mozzarella were collected at an industry in the city of Oliveira. Only three of the five water samples analyzed were suitable for consumption according to Brazilian sanitary standards. Four milk samples were highly contaminated with fecal coliforms, and did not meet the minimal hygienic-sanitary standards according to Brazilian regulations. Only one sample of buffalo muzzarela purchased from retail outlets exceeded the limit for coagulase-positive Staphylococcus. Eleven samples showed counts of thermotolerant coliforms higher than 5 × 103 CFU.g?1, but still lower than the maximum permitted by the Brazilian laws. Salmonella spp. and Listeria monocytogenes were not isolated. Debaryomyces hansenii, Candida lusitaniae and C. parapsilosis were the prevalent yeast species isolated from cheese. Among samples from the production stages, the acidified curd presented the highest numbers of yeasts, with C. catenulata being the most frequent species isolated. Some opportunistic yeast species such as C. guilliermondii, C. tropicalis, C. parapsilosis, C. lusitaniae, C. catenulata, C. rugosa and C. krusei occurred in the mozzarella cheese samples analyzed. The mozzarella cheese presented a low microbial load as compared to other cheese already studied, and the yeast biota included species typical of cheese and also opportunistic pathogens. PMID:24516436

Facchin, Susanne; Barbosa, Anne C.; Carmo, Luiz S.; Silva, Maria Crisolita C.; Oliveira, Afonso L.; Morais, Paula B.; Rosa, Carlos A.

2013-01-01

231

A nonsense mutation in the tyrosinase gene causes albinism in water buffalo  

PubMed Central

Background Oculocutaneous albinism (OCA) is an autosomal recessive hereditary pigmentation disorder affecting humans and several other animal species. Oculocutaneous albinism was studied in a herd of Murrah buffalo to determine the clinical presentation and genetic basis of albinism in this species. Results Clinical examinations and pedigree analysis were performed in an affected herd, and wild-type and OCA tyrosinase mRNA sequences were obtained. The main clinical findings were photophobia and a lack of pigmentation of the hair, skin, horns, hooves, mucosa, and iris. The results of segregation analysis suggest that this disease is acquired through recessive inheritance. In the OCA buffalo, a single-base substitution was detected at nucleotide 1,431 (G to A), which leads to the conversion of tryptophan into a stop codon at residue 477. Conclusion This premature stop codon produces an inactive protein, which is responsible for the OCA buffalo phenotype. These findings will be useful for future studies of albinism in buffalo and as a possible model to study diseases caused by a premature stop codon. PMID:22817390

2012-01-01

232

Yeasts from Water Buffalo Mozzarella, a traditional cheese of the Mediterranean area  

Microsoft Academic Search

Countries of the Mediterranean area are characterized by production of artisanal cheeses, obtained from goat, sheep, cow and buffalo raw milk. The numbers and species of yeasts in the different cheeses are variable, but some species are more frequently detected than others. Kluyveromyces marxianus, K. lactis with their anamorph, Candida kefir, Debaryomyces hansenii and C. famata, C. colliculosa and C.

P Romano; A Ricciardi; G Salzano; G Suzzi

2001-01-01

233

Effects of Smallmouth Buffalo and Potassium Permanganate Treatment on Plankton ans Pond Water Quality  

Technology Transfer Automated Retrieval System (TEKTRAN)

Removal of intermediate hosts is one option for control of disease in channel catfish production systems. We evaluated use of predaceous fish (smallmouth buffalo) and chemical treatment (potassium permanganate) to remove snails that serve as hosts protecting Dero worms. Both methods of treatment r...

234

Habitat selection by African buffalo (Syncerus caffer) in response to landscape-level fluctuations in water availability on two temporal scales.  

PubMed

Seasonal fluctuations in water availability cause predictable changes in the profitability of habitats in tropical ecosystems, and animals evolve adaptive behavioural and spatial responses to these fluctuations. However, stochastic changes in the distribution and abundance of surface water between years can alter resource availability at a landscape scale, causing shifts in animal behaviour. In the Okavango Delta, Botswana, a flood-pulsed ecosystem, the volume of water entering the system doubled between 2008 and 2009, creating a sudden change in the landscape. We used African buffalo (Syncerus caffer) to test the hypotheses that seasonal habitat selection would be related to water availability, that increased floodwater levels would decrease forage abundance and affect habitat selection, and that this would decrease buffalo resting time, reduce reproductive success and decrease body condition. Buffalo selected contrasting seasonal habitats, using habitats far from permanent water during the rainy season and seasonally-flooded habitats close to permanent water during the early and late flood seasons. The 2009 water increase reduced forage availability in seasonally-flooded habitats, removing a resource buffer used by the buffalo during the late flood season, when resources were most limited. In response, buffalo used drier habitats in 2009, although there was no significant change in the time spent moving or resting, or daily distance moved. While their reproductive success decreased in 2009, body condition increased. A protracted period of high water levels could prove detrimental to herbivores, especially to smaller-bodied species that require high quality forage. Stochastic annual fluctuations in water levels, predicted to increase as a result of anthropogenically-induced climate change, are likely to have substantial impacts on the functioning of water-driven tropical ecosystems, affecting environmental conditions within protected areas. Buffer zones around critical seasonal resources are essential to allow animals to engage in compensatory behavioural and spatial mechanisms in response to changing environmental conditions. PMID:24983377

Bennitt, Emily; Bonyongo, Mpaphi Casper; Harris, Stephen

2014-01-01

235

75 FR 61099 - Security Zones; Captain of the Port Buffalo Zone; Technical Amendment  

Federal Register 2010, 2011, 2012, 2013

...Marine safety, Navigation (water), Reporting and record keeping...Zones; Captain of the Port Buffalo Zone. * * * * * (b...contact the Captain of Port Buffalo at telephone number (716...Guard, Captain of the Port Buffalo. [FR Doc. 2010-24774...

2010-10-04

236

Relationships Between Flavoring Capabilities, Bacterial Composition, and Geographical Origin of Natural Whey Cultures Used for Traditional Water-Buffalo Mozzarella Cheese Manufacture  

Microsoft Academic Search

Natural whey cultures (NWC) (n = 29) used for tradi- tional water-buffalo Mozzarella cheese manufacture and arising from different geographical areas of production were characterized and grouped on the basis of their capability to develop neutral volatile compounds and according to their microbial diversity as revealed by mo- lecular analysis. The flavoring properties of NWC were studiedindairymicrocosmsresemblingthespecifictech- nological procedure used

G. Mauriello; L. Moio; A. Genovese; D. Ercolini

2003-01-01

237

Plasma aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH) and gamma-glutamyl transpeptidase (GGT) activities in water buffaloes with experimental subclinical fasciolosis  

Microsoft Academic Search

The effect of chronic Fasciola hepatica infection on the activity of plasma aspartate aminotransferase (AST), glutamate dehydrogenase (GLDH) and gamma-glutamyl transpeptidase (GGT) was investigated in water buffaloes dosed daily with 60 F. hepatica metacercariae over 20 days. Experimental fluke infection caused no clinical signs but provoked an increase in plasma level of IgG directed against F. hepatica from 4 weeks

Qian Yang; Wei Hua Mao; I Ferre; J. E Bayón; X. Z Mao; J González-Gallego

1998-01-01

238

Detection of Mycobacterium avium subsp. paratuberculosis in tissue samples of cattle and buffaloes  

Microsoft Academic Search

Tissue samples were collected at random from cattle (Bos taurus) and buffalo (Bubalus bubalis) from an abattoir of the district of Lahore and were analyzed for the presence of Mycobacterium avium subsp. paratuberculosis and Mycobacterium bovis through acid-fast staining and polymerase chain reaction (PCR). Body condition of animals and diarrhea were recorded. Most\\u000a of the animals were emaciated. Diarrhea was

Farhan Anwar Khan; Zafar Iqbal Chaudhry; Muhammad Ijaz Ali; Shahid Khan; Naima Mumtaz; Ijaz Ahmad

2010-01-01

239

Microarray Analysis of Gene Expression Profiles of Schistosoma japonicum Derived from Less-Susceptible Host Water Buffalo and Susceptible Host Goat  

PubMed Central

Background Water buffalo and goats are natural hosts for S. japonicum in endemic areas of China. The susceptibility of these two hosts to schistosome infection is different, as water buffalo are less conducive to S. japonicum growth and development. To identify genes that may affect schistosome development and survival, we compared gene expression profiles of schistosomes derived from these two natural hosts using high-throughput microarray technology. Results The worm recovery rate was lower and the length and width of worms from water buffalo were smaller compared to those from goats following S. japonicum infection for 7 weeks. Besides obvious morphological difference between the schistosomes derived from the two hosts, differences were also observed by scanning and transmission electron microscopy. Microarray analysis showed differentially expressed gene patterns for parasites from the two hosts, which revealed that genes related to lipid and nucleotide metabolism, as well as protein folding, sorting, and degradation were upregulated, while others associated with signal transduction, endocrine function, development, immune function, endocytosis, and amino acid/carbohydrate/glycan metabolism were downregulated in schistosomes from water buffalo. KEGG pathway analysis deduced that the differentially expressed genes mainly involved lipid metabolism, the MAPK and ErbB signaling pathways, progesterone-mediated oocyte maturation, dorso-ventral axis formation, reproduction, and endocytosis, etc. Conclusion The microarray gene analysis in schistosomes derived from water buffalo and goats provide a useful platform to disclose differences determining S. japonicum host compatibility to better understand the interplay between natural hosts and parasites, and identify schistosome target genes associated with susceptibility to screen vaccine candidates. PMID:23940568

Yang, Jianmei; Hong, Yang; Yuan, Chunxiu; Fu, Zhiqiang; Shi, Yaojun; Zhang, Min; Shen, Liuhong; Han, Yanhui; Zhu, Chuangang; Li, Hao; Lu, Ke; Liu, Jinming; Feng, Xingang; Lin, Jiaojiao

2013-01-01

240

The detection and PCR-based characterization of the parasites causing trypanosomiasis in water-buffalo herds in Venezuela.  

PubMed

The usefulness of PCR-based assays for detecting trypanosomiasis in water buffaloes and other livestock was explored, under field conditions, in Venezuela. The sensitivity and specificity of the assays, which were based on established primer pairs (21-mer/22-mer and ILO1264/ILO1265), were evaluated, partly by comparison with the results of parasitological tests (stained bloodsmears and microhaematocrit centrifugation) and immunological assays (IFAT) run in parallel. The optimised PCR-based assays showed a sensitivity of 10 pg DNA. The use of the 21-mer/22-mer primer pair gave a test that was specific for species in the subgenus Trypanozoon (including Trypanosoma evansi), whereas use of ILO1264/ILO1265 produced a test that was specific for T. vivax. The results of a hybridization assay using T. evansi-DNA and T. vivax-DNA probes indicated no cross-hybridization between the T. evansi and T. vivax PCR products.The results of the bloodsmear examinations, microhaematocrit centrifugations (MHC) and IFAT indicated that 23 (6.7%), 39 (11.4%) and 135 (39.5%) of the 342 blood samples investigated (including 316 from water buffaloes) contained trypanosomes, respectively. The results of the PCR-based assays indicated that 68 (19.9%) of the same blood samples contained T. vivax (or at least T. vivax DNA), and that none contained T. evansi or any other member of the subgenus Trypanozoon. For the detection of trypanosomes, the assay therefore appeared almost twice as sensitive as the MHC. These results are the first on the molecular characterization of the trypanosomes infecting water buffaloes in Venezuela. When the results of the MHC (which is the most practical, and frequently used, alternative detection method) were used as the gold standard, the PCR-based assay for T. vivax was found to have 100% sensitivity, 90.4% specificity, a positive predictive value of 0.57, a positive likelihood ratio of 10.45, and a negative likelihood ratio of 0.00. The assay therefore appears a reasonable choice for detecting T. vivax in the mammalian livestock of Venezuela and elsewhere. PMID:15949183

Garcia, H; Garcia, M-E; Perez, H; Mendoza-Leon, A

2005-06-01

241

Assessment of sperm damages during different stages of cryopreservation in water buffalo by fluorescent probes.  

PubMed

The present study was designed to investigate the sperm damages occurring in acrosome, plasma membrane, mitochondrial activity, and DNA of fresh, equilibrated and frozen-thawed buffalo semen by fluorescent probes. The stability of sperm acrosome and plasma membrane stability, mitochondrial activity and DNA status were assessed by fluorescein conjugated lectin Pisum sativum agglutinin, Annexin-V/propidium iodide, JC-1 and TUNEL assay, respectively, under the fluorescent microscope. The damages percentage of acrosome integrity was significantly increased during equilibration and freezing-thawing process. The stability of sperm plasma membrane is dependent on stability of phosphatidylserine (PS) on the inner leaflet of plasma membrane. The frozen-thawed sperm showed externalization of PS leading to significant increase in apoptotic, early necrotic and necrotic changes and lowered high mitochondrial membrane potential as compared with the fresh sperm but all these parameters were not affected during equilibration. However, the DNA integrity was not affected during equilibration and freezing-thawing procedure. In conclusion, the present study revealed that plasma membrane and mitochondria of buffalo sperm are more susceptible to damage during cryopreservation. Furthermore, the use of fluorescent probes to evaluate integrity of plasma and acrosome membranes, as well as mitochondrial membrane potential and DNA status increased the accuracy of semen analyses. PMID:25373338

Kumar, Dharmendra; Kumar, Pradeep; Singh, Pawan; Yadav, S P; Yadav, P S

2014-11-01

242

Research Article Range and Habitat Selection of African Buffalo  

E-print Network

found that buffalo selected areas within 1 km of water sources, and an isopleth analysis using the new a comparison of buffalo response to water availability in a smaller reserve and important informationResearch Article Range and Habitat Selection of African Buffalo in South Africa SADIE J. RYAN,1

Getz, Wayne M.

243

About Buffalo  

NSDL National Science Digital Library

For people attending our fall conference or summer workshop, we've prepared a list of some of the many things to see and do in and around Buffalo (and just across the border in Canada - Please Note: Travel across the border now requires a passport, for everyone).

2010-01-01

244

Infection rates of Linguatula serrata nymphs in mesenteric lymph nodes from water buffaloes in North India.  

PubMed

The literature pertaining to prevalence of Linguatula serrata in large ruminants is limited. In abattoir survey, the infection rate of L. serrata in 1440 mesenteric lymph nodes collected from 480 buffaloes from North India was investigated. Results revealed 88 (18.3%) buffaloes and 288 (20.0%) mesenteric lymph nodes having parasite's nymphs. Nonsignificant difference (P>0.05), between 1 and 3 years age (51.5%) and above three years of age (48.5%) groups was observed. Nonsignificant difference (P>0.05) between the infection rate of male (51.5%) and female (48.5%) was also observed. Infection in haemorrhagic (57.2%) and black-coloured (67.5%) nymph nodes were significantly (P<0.05) higher than normal-coloured nodes (8.8%). When compared based on consistency, the results showed soft lymph nodes (61.3%) were significantly (P<0.05) more infected than normal (12.8%) and hard (30.0%) lymph nodes. The intensity of infection in normal, haemorrhagic and black lymph nodes were 1.81 ± 0.21, 4.23 ± 0.0.62 and 5.12 ± 0.73, nymphs respectively. The mean numbers of parasites in haemorrhagic and black-coloured lymph nodes were significantly (P<0.0005) more than mean number of parasites in normal-coloured nodes. Again intensity of infection in normal, soft and hard lymph nodes was 2.31 ± 0.18, 5.84 ± 0.74 and 3.21 ± 0.68, respectively. When compared based on lymph nodes consistency, the soft lymph nodes were significantly (P<0.0005) more severely infected than normal and hard ones. The study has generated some vital data about the prevalence of this underreported disease amongst the bubaline intermediate hosts along with important gross changes in the affected lymph nodes. PMID:25112688

Sudan, Vikrant; Jaiswal, Amit Kumar; Shanker, Daya

2014-09-15

245

Genetic studies of water buffalo blood markers. I. Red cell acid phosphatase, albumin, catalase, red cell ?-esterase-3, group-specific component, and protease inhibitor  

Microsoft Academic Search

We have developed the methodologies for typing and family studies to establish the modes of inheritance of water buffalo red cell acid phosphatase (Acp), protease inhibitor (Pi), and group-specific component (Gc) on isoelectric focusing and albumin (Alb), red cell a-esterase-3 (Est-3), and catalase (Cat) on polyacrylamide gel electrophoresis. Family studies showed that Pi, Gc, Alb, and Cat are coded by

S. G. Tan; J. S. F. Barker; O. S. Selvaraj; T. K. Mukherjee; Y. F. Wong

1993-01-01

246

The Schistosoma japonicum self-cure phenomenon in water buffaloes: potential impact on the control and elimination of schistosomiasis in China.  

PubMed

Schistosomiasis japonica, caused by Schistosoma japonicum, is an important zoonotic disease in China, the Philippines and small pockets of Indonesia. In addition to infecting people, S. japonicum can infect over 40 species of wild and domestic animals which have varying impacts on human infection. It is now generally accepted that bovines, particularly water buffaloes, are the major reservoir for human infection in China as they are naturally infected with schistosomes and deposit more eggs into the environment than humans or any other animal host. This complicates control efforts and the economic burden associated with schistosomiasis morbidity and mortality has taken its toll on both human and livestock populations. Over the last 50years, the schistosomiasis control program in China has made great strides in reducing prevalence and morbidity, and the Chinese authorities now aim to eliminate the disease nationwide in the next decade. Current Chinese control strategies place particular importance on interventions targeting bovines including: praziquantel treatment, barrier farming to prevent grazing in transmission areas, their replacement with mechanized tractors and possible bovine vaccination. A number of studies have shown that in the period following S. japonicum infection, the worm burden drops sharply in water buffaloes and some other animal hosts such as pigs. This is due to a self-cure phenomenon whereby there is parasite clearance by both immune and non-immune factors. Here we review studies investigating the self-cure effect, paying particular attention to S. japonicum infection in water buffaloes, and discuss its potential impact on the future schistosomiasis control and elimination efforts in China. Further understanding of the mechanism of self-cure in water buffaloes could be important for future schistosome vaccine design and delivery. PMID:24440417

Li, Yue-Sheng; McManus, Donald P; Lin, Dan-Dan; Williams, Gail M; Harn, Donald A; Ross, Allen G; Feng, Zheng; Gray, Darren J

2014-03-01

247

Molecular and seroepidemiological survey of Babesia bovis and Babesia bigemina infections in cattle and water buffaloes in the central region of Vietnam.  

PubMed

In the present study, a total of 137 blood samples were collected from cattle and water buffaloes in central region of Vietnam and tested using nested polymerase chain reaction (nPCR), enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody test (IFAT) to determine the molecular and serological prevalence of Babesia bovis and Babesia bigemina. In cattle, the prevalence of B. bovis and B. bigemina was 21.3% and 16.0% by nPCR, 73.4% and 42.6% by ELISA and 60.6% and 59.6% by IFAT, respectively, whereas those of water buffalos were 23.3% and 0% by nPCR, 37.2% and 9.3% by ELISA and 27.9% and 18.6% by IFAT, respectively. IFAT and ELISA detected a higher number of infected cattle and water buffaloes than nPCR totally. Statistically significant differences in the prevalence of the two infections were observed on the basis of age. Overall, the current data suggest high incidence of B. bovis and B. bigemina infections in the central region of Vietnam, which is needed to develop comprehensive approach to the modern surveillance, diagnosis and control of bovine babesiosis. PMID:25382466

Li, Yan; Luo, Yuzi; Cao, Shinuo; Terkawi, Mohamad Alaa; Lan, Dinh Thi Bich; Long, Phung Thang; Yu, Longzheng; Zhou, Mo; Gong, Haiyan; Zhang, Houshuang; Zhou, Jinlin; Yokoyama, Naoaki; Suzuki, Hiroshi; Xuan, Xuenan

2014-09-01

248

Clinical and molecular study of a new form of hereditary myotonia in Murrah water buffalo.  

PubMed

Hereditary myotonia caused by mutations in CLCN1 has been previously described in humans, goats, dogs, mice and horses. The goal of this study was to characterize the clinical, morphological and genetic features of hereditary myotonia in Murrah buffalo. Clinical and laboratory evaluations were performed on affected and normal animals. CLCN1 cDNA and the relevant genomic region from normal and affected animals were sequenced. The affected animals exhibited muscle hypertrophy and stiffness. Myotonic discharges were observed during EMG, and dystrophic changes were not present in skeletal muscle biopsies; the last 43 nucleotides of exon-3 of the CLCN1 mRNA were deleted. Cloning of the genomic fragment revealed that the exclusion of this exonic sequence was caused by aberrant splicing, which was associated with the presence of a synonymous SNP in exon-3 (c.396C>T). The mutant allele triggered the efficient use of an ectopic 5' splice donor site located at nucleotides 90-91 of exon-3. The predicted impact of this aberrant splicing event is the alteration of the CLCN1 translational reading frame, which results in the incorporation of 24 unrelated amino acids followed by a premature stop codon. PMID:23339992

Borges, Alexandre S; Barbosa, José D; Resende, Luiz Antônio L; Mota, Lígia S L S; Amorim, Rogério M; Carvalho, Thaís L; Garcia, José F; Oliveira-Filho, José P; Oliveira, Carlos M C; Souza, Jorge Estefano S; Winand, Nena J

2013-03-01

249

33 CFR 117.955 - Buffalo Bayou.  

Code of Federal Regulations, 2011 CFR

...Navigable Waters 1 2011-07-01 2011-07-01 false Buffalo Bayou. 117.955 Section 117.955 Navigation and Navigable...REGULATIONS Specific Requirements Texas § 117.955 Buffalo Bayou. (a) The draw of the Houston Belt and...

2011-07-01

250

33 CFR 117.955 - Buffalo Bayou.  

Code of Federal Regulations, 2010 CFR

...Navigable Waters 1 2010-07-01 2010-07-01 false Buffalo Bayou. 117.955 Section 117.955 Navigation and Navigable...REGULATIONS Specific Requirements Texas § 117.955 Buffalo Bayou. (a) The draw of the Houston Belt and...

2010-07-01

251

33 CFR 117.955 - Buffalo Bayou.  

Code of Federal Regulations, 2012 CFR

...Navigable Waters 1 2012-07-01 2012-07-01 false Buffalo Bayou. 117.955 Section 117.955 Navigation and Navigable...REGULATIONS Specific Requirements Texas § 117.955 Buffalo Bayou. (a) The draw of the Houston Belt and...

2012-07-01

252

33 CFR 117.955 - Buffalo Bayou.  

Code of Federal Regulations, 2013 CFR

...Navigable Waters 1 2013-07-01 2013-07-01 false Buffalo Bayou. 117.955 Section 117.955 Navigation and Navigable...REGULATIONS Specific Requirements Texas § 117.955 Buffalo Bayou. (a) The draw of the Houston Belt and...

2013-07-01

253

Effect of the stage of estrous cycle on follicular population, oocyte yield and quality, and biochemical composition of serum and follicular fluid in Anatolian water buffalo.  

PubMed

This study was designed to investigate the effects that the different stages of the estrous cycle had on the number of surface ovarian follicles and oocyte yield and quality of Anatolian water buffalo during peak breeding season. Assessments were made on the basis of ovarian morphology, serum and follicular fluid concentrations of variety of biochemical parameters. Following slaughter, blood samples were collected from each animal. The stage of estrous cycle was classified as either the luteal or follicular phase, and surface ovarian follicles were classified as small, medium, or large. The follicular fluid was aspirated, and oocytes were evaluated microscopically for classification into four categories. No statistical differences (p>0.05) were observed regarding the total number of follicles or quality of oocytes relative to the stage of the estrous cycle. Serum high-density lipoprotein cholesterol (HDL), alkaline phosphatase (ALP) and progesterone (P4) concentrations were significantly higher in the luteal phase than in the follicular phase (P<0.05). Significant correlations were observed in the luteal phase between the total number of oocytes and cholesterol (Cho), HDL, sodium (Na), chloride (Cl); A-quality oocytes and Na, Cl, Mg; C-quality oocytes and Cho, HDL, and Mg in follicular fluid. These results offer new information concerning Anatolian water buffalo reproductive physiology, which may be useful for improving oocyte quality in buffalo. This is the first study to describe the number of ovarian follicles, oocyte yield and quality, and a variety of biochemical parameters in the serum and follicular fluid of Anatolian water buffalo during peak breeding season in Turkey. PMID:23317849

Baki Acar, Duygu; Birdane, Muhammed Kursad; Dogan, Nurhan; Gurler, Hande

2013-02-01

254

Studies on the bacteriological qualities of the Buffalo River and three source water dams along its course in the Eastern Cape Province of South Africa.  

PubMed

The Buffalo River and its dams are major surface water sources used for fresh produce irrigation, raw water abstraction and recreation in parts of the Eastern Cape Province in South Africa. Over a 12-month period (August 2010 to July 2011), we assessed the bacteriological qualities of water from the river and 3 source water dams along its course. Faecal indicator bacteria (FIB), including total coliform (TC), faecal coliform (FC) and enterococci (ENT) counts, were high and ranged as follows: 1.9 × 10(2)-3.8 × 10(7), 0-3.0 × 10(5) and 0-5.3 × 10(5) cfu/100 ml for TC, FC and ENT, respectively. Significantly (P<0.05) higher concentrations of FC and ENT were observed at the sampling sites located at the lower reaches of the river compared to the upper reaches, and at Bridle Drift Dam compared to the other two dams. FIB counts mostly exceeded the recommended maximum values suggested by national and international guidelines for safe fresh produce irrigation, domestic applications, full-contact recreation and livestock watering. These results show that the bacteriological qualities of the Buffalo River and dams were poor, and suggest that sewage was dumped into the Buffalo River during the study period. Urban runoffs and effluents of wastewater treatment plants appear to be important sources of faecal contamination in the river. We conclude that these water bodies represent significant public health hazards. Provision of adequate sanitary infrastructure will help prevent source water contamination, and public health education aimed at improving personal, household and community hygiene is imperative. PMID:23238595

Chigor, Vincent N; Sibanda, Timothy; Okoh, Anthony I

2013-06-01

255

Prevalence and antimicrobial susceptibility of Arcobacter species in cow milk, water buffalo milk and fresh village cheese.  

PubMed

In this study, the presence of Arcobacter spp. was examined in cow milk (n=50), water buffalo (WB) milk (n=50) and fresh village cheese (n=50) samples. The 16S rDNA-RFLP method was used for the identification of Arcobacter spp. The disc diffusion method was used to investigate the susceptibility of all strains identified to 18 different antimicrobial substances. The most commonly isolated Arcobacter species were found to be Arcobacter butzleri (38.89%), Arcobacter cryaerophilus (22.23%) and Arcobacter skirrowii (11.12%) in cow milk; A. cryaerophilus (33.33%), Arcobacter cibarius (20.83%) and A. butzleri (12.50%) in WB milk; and A. skirrowii (28.57%), A. butzleri (21.43%) and A. cryaerophilus (14.29%) in fresh village cheese. This is the first study to identify the presence of Arcobacter nitrofigilis, Arcobacter cloacae, Arcobacter halophilus, Arcobacter bivalviorum and A. cibarius species in analyzed samples. It was found that all of the A. cryaerophilus (n:16) isolates were resistant to cefoperazone, cloxacillin and penicillin G; all of the A. skirrowii (n:12) and A. butzleri (n:10) isolates were resistant to cefoperazone, tetracycline, ampicillin, erythromycin, cloxacillin and penicillin G. It was concluded that cow milk, WB milk and fresh village cheese samples are an important source of Arcobacter species and pose a risk to public health. PMID:25064812

Yesilmen, Simten; Vural, Aydin; Erkan, Mehmet Emin; Yildirim, Ibrahim Halil

2014-10-01

256

Assessment of the risks for human health of adenoviruses, hepatitis A virus, rotaviruses and enteroviruses in the Buffalo River and three source water dams in the Eastern Cape.  

PubMed

Buffalo River is an important water resource in the Eastern Cape Province of South Africa. The potential risks of infection constituted by exposure to human enteric viruses in the Buffalo River and three source water dams along its course were assessed using mean values and static quantitative microbial risk assessment (QMRA). The daily risks of infection determined by the exponential model [for human adenovirus (HAdV) and enterovirus (EnV)] and the beta-Poisson model (for hepatitis A virus (HAV) and rotavirus (RoV)) varied with sites and exposure scenario. The estimated daily risks of infection values at the sites where the respective viruses were detected, ranged from 7.31 × 10(-3) to 1 (for HAdV), 4.23 × 10(-2) to 6.54 × 10(-1) (RoV), 2.32 × 10(-4) to 1.73 × 10(-1) (HAV) and 1.32 × 10(-4) to 5.70 × 10(-2) (EnV). The yearly risks of infection in individuals exposed to the river/dam water via drinking, recreational, domestic or irrigational activities were unacceptably high, exceeding the acceptable risk of 0.01% (10(-4) infection/person/year), and the guideline value used as by several nations for drinking water. The risks of illness and death from infection ranged from 6.58 × 10(-5) to 5.0 × 10(-1) and 6.58 × 10(-9) to 5.0 × 10(-5), respectively. The threats here are heightened by the high mortality rates for HAV, and its endemicity in South Africa. Therefore, we conclude that the Buffalo River and its source water dams are a public health hazard. The QMRA presented here is the first of its kinds in the Eastern Cape Province and provides the building block for a quantitatively oriented local guideline for water quality management in the Province. PMID:24676673

Chigor, Vincent N; Sibanda, Timothy; Okoh, Anthony I

2014-06-01

257

176 blood flow to the corpus luteum and preovulatory follicle after ovulation induction during first versus second wave in water buffalo.  

PubMed

The objective of the study was to compare the blood flow to the corpus luteum (CL) and the preovulatory follicle in dairy buffalo (Bubalus bubalis) when ovulation was induced during the first (low to increasing progesterone levels) versus the second (luteal progesterone levels) follicular wave. We hypothesised that the wall of the first-wave dominant follicle will be less vascular compared with that of the second-wave follicle. The study was conducted during the summer months in Punjab, India. Ovulation was synchronized with prostaglandin F2? (PGF) IM followed by gonadotropin-releasing hormone (GnRH) IM 48h later (Day 0) and buffaloes were randomised to first wave (FW; n=6) and second wave (SW; n=7) groups. FW group was given PGF on Days 6.5 and 7, and GnRH on Day 9.5 followed by AI (14-16h after GnRH). The SW group was given GnRH on Day 7 (to induce ovulation of first-wave dominant follicle without luteolysis and synchronous emergence of next wave), PGF on Days 13.5 and 14, GnRH on Day 16.5 followed by artificial insemination. Transrectal colour Doppler ultrasonography (MyLab5 Vetwith 7.5MHz transducer, Esaote S.p.A, Genoa, Italy) was performed daily and 20-s cineloops of each ovary were recorded under standardized gain controls. Images from the cineloops were processed using Fiji (ImageJ, National Institutes of Health, Bethesda, MD, USA) to calculate the area of blood flow (coloured area=vascular area, grey scale area=tissue area, and their ratio) for the preovulatory follicle (on the day before ovulation) and luteal tissue (on the day of PGF injection and 4 days post-ovulation). Data were analysed by t-test from the animals that ovulated one day before (n=3) or the day of AI (n=6) and had a functional CL at day 5 post-AI (FW n=5, SW n=4). FW follicles ovulated on 8.6±0.3 days from wave emergence compared with SW follicles on 10.0±0.6 days (P<0.05) but were similar in size (i.e. follicular area on the day before ovulation did not differ between groups; P=0.5). There was no difference in the blood flow area in the wall of preovulatory follicles (P=0.4). Vascular area of follicles was strongly correlated with their diameter (r=0.87). Follicles >13.5mm in diameter had more blood flow in their wall than smaller follicles (P<0.01). FW had a tendency (P=0.07) for smaller luteal area on the day of PGF treatment (FW=171±24mm(2); SW=332±81mm(2)) and tended (P=0.06) to have less vascular area in the CL compared to SW group (FW=30±6mm(2); SW=67±17mm(2)). There was no difference (P=0.5) between the groups for vascular to CL area ratio. The area of luteal tissue and blood flow to the CL at Day 4 post-ovulation did not differ between the groups (P=0.4). The diameter of the preovulatory follicle (11.6-15.7mm) was not correlated with the cross-sectional area of developing CL at Day 4 post-ovulation (r=0.09). In conclusion, vascularity to preovulatory follicles originating from the first wave v. second wave did not differ and preovulatory follicles ?13.5mm were more vascular than smaller follicles. PMID:25472225

Caunce, S; Dadarwal, D; Brar, P S; Singh, J

2014-12-01

258

First molecular characterization of Cryptosporidium and Giardia from bovines (Bos taurus and Bubalus bubalis) in Sri Lanka: unexpected absence of C. parvum from pre-weaned calves  

PubMed Central

Background The genetic characterization of Cryptosporidium and Giardia has important implications for investigating their epidemiology and underpins their control. We undertook the first molecular epidemiological survey of domestic bovids in selected regions of Sri Lanka to establish whether they excreted Cryptosporidium and/or Giardia with zoonotic potential. Methods Faecal samples were collected from dairy calves (n?=?340; Bos taurus; < 3 months of age; weekly sampling for six weeks) and water buffaloes (n?=?297; Bubalus bubalis; <6 months and ?6 months of age; one sampling) from seven different farms in Sri Lanka. Genomic DNAs were extracted from individual faecal samples and then tested for the presence of parasite DNA using a PCR-based mutation scanning-targeted sequencing-phylogenetic approach, employing genetic markers within the small subunit of nuclear ribosomal RNA and 60 kDa glycoprotein genes (designated pSSU and pgp60, respectively) for Cryptosporidium, and within the triose phosphate isomerise (ptpi) gene for Giardia. Results Based on pSSU sequence data, C. bovis, C. ryanae and six new genotypes that were genetically similar but not identical to C. andersoni (n?=?1), C. bovis (n?=?1), C. ryanae (n?=?3) and C. suis (n?=?1) were recorded in cattle. For pSSU, two other, new genotypes were defined in water buffalo, which were genetically most similar to Cryptosporidium genotypes recorded previously in this host species in other countries including Australia. Consistent with the findings for pSSU, no species or genotypes of Cryptosporidium with zoonotic potential were detected using pgp60. Based on ptpi sequence data, G. duodenalis assemblages A and E were detected in four and 137 samples from cattle, respectively, and assemblage E in two samples from water buffaloes. Conclusions The present study showed that C. parvum, the most commonly reported zoonotic species of Cryptosporidium recognised in bovine calves globally, was not detected in any of the samples from pre-weaned calves tested in the present study. However, eight new genotypes were recorded. Future studies of different host species in various regions are required to investigate the molecular epidemiology of cryptosporidiosis and giardiasis in Sri Lanka and neighbouring countries in South Asia. PMID:24559043

2014-01-01

259

Identification of virulence factors in 16S-23S rRNA intergenic spacer genotyped Staphylococcus aureus isolated from water buffaloes and small ruminants.  

PubMed

Staphylococcus aureus is an important human and animal pathogen, and is regarded as an important cause of intramammary infection (IMI) in ruminants. Staphylococcus aureus genetic variability and virulence factors have been well studied in veterinary medicine, especially in cows as support for control and management of IMI. The aim of the present study was to genotype 71 Staph. aureus isolates from the bulk tank and foremilk of water buffaloes (n=40) and from udder tissue (n=7) and foremilk (n=24) from small ruminants. The method used was previously applied to bovine Staph. aureus and is based on the amplification of the 16S-23S rRNA intergenic spacer region. The technique applied was able to identify different Staph. aureus genotypes isolated from dairy species other than the bovine species, and cluster the genotypes according to species and herds. Virulence gene distribution was consistent with genotype differentiation. The isolates were also characterized through determination of the presence of 19 virulence-associated genes by specific PCR. Enterotoxins A, C, D, G, I, J, and L were associated with Staph. aureus isolates from buffaloes, whereas enterotoxins C and L were linked to small ruminants. Genes coding for methicillin resistance, Panton-Valentine leukocidin, exfoliative toxins A and B, and enterotoxins B, E, and H were undetected. These findings indicate that RNA template-specific PCR is a valid technique for typing Staph. aureus from buffaloes and small ruminants and is a useful tool for understanding udder infection epidemiology. PMID:24140323

Cremonesi, P; Zottola, T; Locatelli, C; Pollera, C; Castiglioni, B; Scaccabarozzi, L; Moroni, P

2013-12-01

260

Calcium and magnesium content of the uterine fluid and blood serum during the estrous cycle and pre-pubertal phase in water buffaloes.  

PubMed

To investigate uterine fluid and serum calcium (Ca) and Magnesium (Mg) variations during the estrus cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by examining ovarian structures. 18 animals were pro-estrous, 15 estrous, 16 met-estrous and 22 diestrous. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. Blood serum and uterine fluid samples of 71 pre-pubertal buffalo calves were also collected and treated in similar manners. The mean ± SEM total serum and uterine fluid Ca in cyclic buffaloes were 8.68 ± 0.28 mg dL(-1) and 8.10 ± 0.2 mg dL(-1) vs. 6.76 ± 0.65 mg dL(-1) and 7.90 ± 0.15 mg dL(-1) in pre-pubertal calves, respectively. Blood serum Mg was not different in cyclic and pre-pubertal animals but the uterine fluid Mg in cyclic cows was higher than those in pre-pubertal calves. Serum Ca in pro-estrus and estrus were higher than those in other stages and also higher than those in the uterine fluid. The lowest Mg content of serum was recorded in diestrus, while in the uterine fluid it was observed in estrus. In all stages of estrous cycle except for estrus the uterine fluid Mg content was significantly higher than those of the serum. These results suggested that during the estrous cycle in the buffalo cows, Ca was passively secreted in uterine lumen and mostly dependent on blood serum Ca concentrations but Mg was secreted independently. The values (except for serum total Mg) also increased after puberty. PMID:25610582

Alavi Shoushtari, Sayed Mortaza; Asri Rezaie, Siamak; Khaki, Amir; Belbasi, Abulfazle; Tahmasebian, Hamid

2014-01-01

261

Calcium and magnesium content of the uterine fluid and blood serum during the estrous cycle and pre-pubertal phase in water buffaloes  

PubMed Central

To investigate uterine fluid and serum calcium (Ca) and Magnesium (Mg) variations during the estrus cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by examining ovarian structures. 18 animals were pro-estrous, 15 estrous, 16 met-estrous and 22 diestrous. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. Blood serum and uterine fluid samples of 71 pre-pubertal buffalo calves were also collected and treated in similar manners. The mean ± SEM total serum and uterine fluid Ca in cyclic buffaloes were 8.68 ± 0.28 mg dL-1 and 8.10 ± 0.2 mg dL-1 vs. 6.76 ± 0.65 mg dL-1 and 7.90 ± 0.15 mg dL-1 in pre-pubertal calves, respectively. Blood serum Mg was not different in cyclic and pre-pubertal animals but the uterine fluid Mg in cyclic cows was higher than those in pre-pubertal calves. Serum Ca in pro-estrus and estrus were higher than those in other stages and also higher than those in the uterine fluid. The lowest Mg content of serum was recorded in diestrus, while in the uterine fluid it was observed in estrus. In all stages of estrous cycle except for estrus the uterine fluid Mg content was significantly higher than those of the serum. These results suggested that during the estrous cycle in the buffalo cows, Ca was passively secreted in uterine lumen and mostly dependent on blood serum Ca concentrations but Mg was secreted independently. The values (except for serum total Mg) also increased after puberty. PMID:25610582

Alavi Shoushtari, Sayed Mortaza; Asri Rezaie, Siamak; Khaki, Amir; Belbasi, Abulfazle; Tahmasebian, Hamid

2014-01-01

262

33 CFR 162.165 - Buffalo and Rochester Harbors, New York.  

Code of Federal Regulations, 2011 CFR

...Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Buffalo and Rochester Harbors...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...REGULATIONS § 162.165 Buffalo and Rochester...

2011-07-01

263

33 CFR 162.165 - Buffalo and Rochester Harbors, New York.  

Code of Federal Regulations, 2012 CFR

...Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Buffalo and Rochester Harbors...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...REGULATIONS § 162.165 Buffalo and Rochester...

2012-07-01

264

33 CFR 162.165 - Buffalo and Rochester Harbors, New York.  

Code of Federal Regulations, 2013 CFR

...Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Buffalo and Rochester Harbors...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...REGULATIONS § 162.165 Buffalo and Rochester...

2013-07-01

265

33 CFR 162.165 - Buffalo and Rochester Harbors, New York.  

...Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Buffalo and Rochester Harbors...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...REGULATIONS § 162.165 Buffalo and Rochester...

2014-07-01

266

33 CFR 162.165 - Buffalo and Rochester Harbors, New York.  

Code of Federal Regulations, 2010 CFR

...Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Buffalo and Rochester Harbors...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...REGULATIONS § 162.165 Buffalo and Rochester...

2010-07-01

267

33 CFR 117.955 - Buffalo Bayou.  

...Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY BRIDGES DRAWBRIDGE OPERATION REGULATIONS Specific Requirements Texas § 117.955 Buffalo Bayou. (a) The draw of the Houston Belt and Terminal railroad bridge, mile 1.2 at...

2014-07-01

268

Escherichia coli O157:H7/NM prevalence in raw beef, camel, sheep, goat, and water buffalo meat in Fars and Khuzestan provinces, Iran  

PubMed Central

Enterohemorrhagic Escherichia coli (EHEC) of the O157:H7 serotype is a worldwide zoonotic pathogen responsible for the majority of severe cases of human EHEC disease. The aim of the present study was to investigate the prevalence of E. coli O157: H7/NM in raw meat samples from two provinces of Iran. During a period from March 2010 to March 2011. Two hundred and ninety five raw meat samples were collected from beef (n= 85), camel, (n= 50), sheep (n= 62), goat (n= 60), and water buffalo (n=38). Fourteen (4.7%) of the 295 samples were positive for E. coli O157. The highest prevalence of E. coli O157 was found in beef samples (8.2%), followed by water buffalo (5.3%), sheep (4.8%), camel (2.0%), and goat (1.7%). Of fourteen E. coli O157 isolates, only one was determined to be serotype O157: H7 while 13 were determined as serotype O157: NM. Of the 14 E. coli O157:H7/NM isolates, one, four, two, and one strains were positive for stx1, stx2, eaeA and ehlyA genes, respectively. The prevalence of this organism varied between seasons with the highest prevalence of E. coli O157 occurring in summer (9.3%). The results of this study showed that beef and water buffalo meat are a significant source for human EHEC E. coli O157:H7/NM infection in Iran. The data reported in this study provides some useful baseline in formation for future research such as molecular or epidemiologic works.

Rahimi, Ebrahim; Kazemeini, Hamid Reza; Salajegheh, Mohammad

2012-01-01

269

Decline of Year-Class Strength of Buffalo Fishes in Lake Sakakawea,  

E-print Network

, and reproduction of buffalo fishes under reservoir conditions is controlled by fluctuating water levels and quality are not uniformly available each year. Quality of spawning beds also is related to water levels, because buffalo and smallmouth buffalo reproduction i:-. closely related to water levels. The year-classes produced before

270

Effect of cooking and storage on lipid oxidation and development of cholesterol oxidation products in water buffalo meat  

Microsoft Academic Search

Buffalo meat was subjected to two cooking methods viz. broiling and pressure cooking and two storage procedures viz. refrigerated (4 °C) storage for six days and frozen (?10 °C) storage for 90 days. Changes in lipid oxidation and development of cholesterol oxidation products were studied in raw as well as cooked meat samples. Total lipid, phospholipid, cholesterol, free fatty acid,

V. Kesava Rao; B. N. Kowale; N. P. Babu; G. S. Bisht

1996-01-01

271

The buffalo wars  

E-print Network

The wandering buffalo of Yellowstone National Park are the subject of a heated debate in the western United States. The animals carry a disease called brucellosis, which infects both buffalo and cattle and has economic ...

Nasr, Susan L

2006-01-01

272

A Global View of 54,001 Single Nucleotide Polymorphisms (SNPs) on the Illumina BovineSNP50 BeadChip and Their Transferability to Water Buffalo  

E-print Network

The Illumina BovineSNP50 BeadChip features 54,001 informative single nucleotide polymorphisms (SNPs) that uniformly span the entire bovine genome. Among them, 52,255 SNPs have locations assigned in the current genome assembly (Btau_4.0), including 19,294 (37%) intragenic SNPs (i.e., located within genes) and 32,961 (63%) intergenic SNPs (i.e., located between genes). While the SNPs represented on the Illumina Bovine50K BeadChip are evenly distributed along each bovine chromosome, there are over 14,000 genes that have no SNPs placed on the current BeadChip. Kernel density estimation, a non-parametric method, was used in the present study to identify SNP-poor and SNP-rich regions on each bovine chromosome. With bandwidth = 0.05 Mb, we observed that most regions have SNP densities within 2 standard deviations of the chromosome SNP density mean. The SNP density on chromosome X was the most dynamic, with more than 30 SNP-rich regions and at least 20 regions with no SNPs. Genotyping ten water buffalo using the Illumina BovineSNP50 BeadChip revealed that 41,870 of the 54,001 SNPs are fully scored on all ten water buffalo, but 6,771 SNPs are partially scored on one to nine animals. Both fully scored and partially/no

Vanessa N. Michelizzi; Xiaolin Wu; Michael V. Dodson; Jennifer J. Michal; Jorge Zambrano-varon; Derek J. Mclean; Zhihua Jiang

273

33 CFR 207.580 - Buffalo Harbor, N.Y.; use, administration, and navigation.  

...Navigable Waters 3 2014-07-01 2014-07-01 false Buffalo Harbor, N.Y.; use, administration, and navigation...DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.580 Buffalo Harbor, N.Y.; use, administration, and...

2014-07-01

274

33 CFR 207.580 - Buffalo Harbor, N.Y.; use, administration, and navigation.  

Code of Federal Regulations, 2011 CFR

...Navigable Waters 3 2011-07-01 2011-07-01 false Buffalo Harbor, N.Y.; use, administration, and navigation...DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.580 Buffalo Harbor, N.Y.; use, administration, and...

2011-07-01

275

33 CFR 207.580 - Buffalo Harbor, N.Y.; use, administration, and navigation.  

Code of Federal Regulations, 2012 CFR

...Navigable Waters 3 2012-07-01 2012-07-01 false Buffalo Harbor, N.Y.; use, administration, and navigation...DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.580 Buffalo Harbor, N.Y.; use, administration, and...

2012-07-01

276

33 CFR 207.580 - Buffalo Harbor, N.Y.; use, administration, and navigation.  

Code of Federal Regulations, 2010 CFR

...Navigable Waters 3 2010-07-01 2010-07-01 false Buffalo Harbor, N.Y.; use, administration, and navigation...DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.580 Buffalo Harbor, N.Y.; use, administration, and...

2010-07-01

277

33 CFR 207.580 - Buffalo Harbor, N.Y.; use, administration, and navigation.  

Code of Federal Regulations, 2013 CFR

...Navigable Waters 3 2013-07-01 2013-07-01 false Buffalo Harbor, N.Y.; use, administration, and navigation...DEPARTMENT OF DEFENSE NAVIGATION REGULATIONS § 207.580 Buffalo Harbor, N.Y.; use, administration, and...

2013-07-01

278

Searching for copy number variations in the buffalo genome  

Technology Transfer Automated Retrieval System (TEKTRAN)

Water buffalo are economically important animals in many regions of the world, especially in developing countries. The International Water Buffalo Consortium will sequence an Italian inbred female (33-fold coverage) to build the genome assembly and additional individuals to screen for SNPs. Based on...

279

Quantitative RT-PCR Detection of Hepatitis A Virus, Rotaviruses and Enteroviruses in the Buffalo River and Source Water Dams in the Eastern Cape Province of South Africa  

PubMed Central

Human enteric viruses (HEntVs) are a major cause of water-related diseases. The prevalence of hepatitis A virus (HAV), rotaviruses (RoV) and enteroviruses (EnV) in Buffalo River waters was assessed quantitatively over a period of 12 months (August 2010 to July 2011). Seventy-two samples were collected from six sites, including three dams, and concentrated using the adsorption-elution method. Viral RNA was extracted using a commercial kit, and the viruses were quantified by real-time quantitative reverse transcriptase PCR (RT-qPCR). Two or more viruses were detected in 12.5% of the samples. HAV was detected in 43.1% of the samples and in significantly (p < 0.05) varying concentrations of 1.5 × 101–1.9 × 105 genome copies/L compared to RoV and EnV, while RoVs were detected in 13.9% of samples, with concentrations ranging from 2.5 × 101–2.1 × 103 genome copies/L, and EnV were detected in 9.7% of the samples, with concentrations ranging from 1.3 × 101–8.6 × 101 genome copies/L. Only HAV was detected at all the sites, with the Bridle Drift Dam recording significantly higher (p < 0.05) concentrations. The presence of enteric viruses in Buffalo River may constitute public health risks and the incidence of HAV at all the sites could reflect both the epidemiological status of hepatitis A and HAV persistence in the water environments. PMID:23202829

Chigor, Vincent Nnamdigadi; Okoh, Anthony Ifeanyi

2012-01-01

280

Thermal Band Characterization of LANDSAT-4 Thematic Mapper. [Buffalo, New York and water temperature in Lake Erie  

NASA Technical Reports Server (NTRS)

A quick look monitor in the spacecraft control center was used to measure the TM Band 6 shutter background and the 34.7 C internal blackbody signal on over 50 dates. Comparison of relative internal gains between the four channels to prelaunch values showed changes over 9 months of up to 5%, while 512 x 512 subsections of the original 10 daytime scenes showed scene counts that ranged from 135 down to 62. A night scene of the Buffalo area was used to determine channel gain relative to the mean and to discern a systematic along scan pattern in a difference between forward and reverse scan counts of up to 0.5. A corrected digital image was produced and individual gains and offsets were calculated for the four channels. At satellite radiance was determine and noise equivalent temperature difference was calculated. The calibration data and the Buffalo scene, with the corrections and estimates of the atmospheric transmission and radiance, were used to make a temperature estimate for an area of Lake Erie of 21 C to 27 C. Local records of the temperature showed 21 C.

Lansing, J. C.; Barker, J. L.

1984-01-01

281

33 CFR 110.208 - Buffalo Harbor, N.Y.  

Code of Federal Regulations, 2013 CFR

...Navigable Waters 1 2013-07-01 2013-07-01 false Buffalo Harbor, N.Y. 110.208 Section 110.208 Navigation... ANCHORAGE REGULATIONS Anchorage Grounds § 110.208 Buffalo Harbor, N.Y. (a) The anchorage grounds —(1)...

2013-07-01

282

33 CFR 110.208 - Buffalo Harbor, N.Y.  

...Navigable Waters 1 2014-07-01 2014-07-01 false Buffalo Harbor, N.Y. 110.208 Section 110.208 Navigation... ANCHORAGE REGULATIONS Anchorage Grounds § 110.208 Buffalo Harbor, N.Y. (a) The anchorage grounds —(1)...

2014-07-01

283

33 CFR 110.208 - Buffalo Harbor, N.Y.  

Code of Federal Regulations, 2012 CFR

...Navigable Waters 1 2012-07-01 2012-07-01 false Buffalo Harbor, N.Y. 110.208 Section 110.208 Navigation... ANCHORAGE REGULATIONS Anchorage Grounds § 110.208 Buffalo Harbor, N.Y. (a) The anchorage grounds —(1)...

2012-07-01

284

33 CFR 110.208 - Buffalo Harbor, N.Y.  

Code of Federal Regulations, 2011 CFR

...Navigable Waters 1 2011-07-01 2011-07-01 false Buffalo Harbor, N.Y. 110.208 Section 110.208 Navigation... ANCHORAGE REGULATIONS Anchorage Grounds § 110.208 Buffalo Harbor, N.Y. (a) The anchorage grounds —(1)...

2011-07-01

285

TEMPERATURE AND CONDUCTIVITY MODELING FOR THE BUFFALO RIVER  

EPA Science Inventory

A hydrodynamic and water quality transport study of the Buffalo River has been conducted. sing a two-dimensional (laterally averaged) model and incorporating appropriate specification of boundary conditions, we simulated the transport of river water temperature and conductivity f...

286

Mechanical and Aerospace Engineering University at Buffalo  

E-print Network

Mechanical and Aerospace Engineering University at Buffalo S t a t e U n i v e r s i t y o f N e w Y o r k MAE Seminar Series Nanoengineered Surfaces for Efficiency Enhancements in Energy and Water Energy, Water, Agriculture, Transportation, Electronics Cooling, Buildings, etc. Over the years

Krovi, Venkat

287

The effect of buffalo CD14 shRNA on the gene expression of TLR4 signal pathway in buffalo monocyte/macrophages.  

PubMed

CD14 plays a crucial role in the inflammatory response to lipopolysaccharide (LPS), which interacts with TLR4 and MD-2 to enable cell activation, resulting in inflammation. Upstream inhibition of the inflammation pathway mediated by bacterial LPS, toll-like receptor 4 (TLR4) and cluster of differentiation antigen 14 (CD14) was proven to be an effective therapeutic approach for attenuating harmful immune activation. To explore the effect of CD14 downregulation on the expression of TLR4 signaling pathway-related genes after LPS stimulation in buffalo (Bubalus bubalis) monocyte/macrophages, effective CD14 shRNA sequences were screened using qRT-PCR and FACS analysis with buffalo CD14 shRNA lentiviral recombinant plasmids (pSicoRGFP-shRNA) and buffalo CD14 fusion expression plasmids (pDsRed-N1-buffalo CD14) co-transfected into HEK293T cells via liposomes. Of the tested shRNAs, shRNA-1041 revealed the highest knockdown efficiency (p < 0.01). When buffalo peripheral blood monocyte/macrophages were infected with shRNA-1041 lentivirus and stimulated with LPS, the expression of endogenous CD14 was significantly decreased by CD14 shRNA (p < 0.01), and the mRNA expression levels of TLR4, IL-6 and TNF-? were also significantly downregulated compared to the control groups (p < 0.01). These results demonstrated that the knockdown of endogenous CD14 had clear regulatory effects on the signal transduction of TLR4 after stimulation with LPS. These results may provide a better understanding of the molecular mechanisms of CD14 regulation in the development of several buffalo diseases. PMID:25355240

Li, Xiangping; Li, Meiqing; Huang, Shihai; Qiao, Shuye; Qin, Zhaoxian; Kang, Chao; Shi, Deshun

2014-12-01

288

33 CFR 3.45-10 - Sector Buffalo Marine Inspection Zone and Captain of the Port Zone.  

Code of Federal Regulations, 2012 CFR

...Navigation and Navigable Waters 1 2012-07-01...2012-07-01 false Sector Buffalo Marine Inspection...office is located in Buffalo, NY. The boundaries of Sector Buffalo's Marine Inspection...include all navigable waters of the United States and contiguous...

2012-07-01

289

33 CFR 3.45-10 - Sector Buffalo Marine Inspection Zone and Captain of the Port Zone.  

Code of Federal Regulations, 2011 CFR

...Navigation and Navigable Waters 1 2011-07-01...2011-07-01 false Sector Buffalo Marine Inspection...office is located in Buffalo, NY. The boundaries of Sector Buffalo's Marine Inspection...include all navigable waters of the United States and contiguous...

2011-07-01

290

33 CFR 3.45-10 - Sector Buffalo Marine Inspection Zone and Captain of the Port Zone.  

...Navigation and Navigable Waters 1 2014-07-01...2014-07-01 false Sector Buffalo Marine Inspection...office is located in Buffalo, NY. The boundaries of Sector Buffalo's Marine Inspection...include all navigable waters of the United States and contiguous...

2014-07-01

291

33 CFR 3.45-10 - Sector Buffalo Marine Inspection Zone and Captain of the Port Zone.  

Code of Federal Regulations, 2013 CFR

...Navigation and Navigable Waters 1 2013-07-01...2013-07-01 false Sector Buffalo Marine Inspection...office is located in Buffalo, NY. The boundaries of Sector Buffalo's Marine Inspection...include all navigable waters of the United States and contiguous...

2013-07-01

292

33 CFR 3.45-10 - Sector Buffalo Marine Inspection Zone and Captain of the Port Zone.  

Code of Federal Regulations, 2010 CFR

...Navigation and Navigable Waters 1 2010-07-01...2010-07-01 false Sector Buffalo Marine Inspection...office is located in Buffalo, NY. The boundaries of Sector Buffalo's Marine Inspection...include all navigable waters of the United States and contiguous...

2010-07-01

293

Anti-fecundity immunity to Schistosoma japonicum induced in Chinese water buffaloes ( Bos buffelus) after vaccination with recombinant 26 kDa glutathione- S-transferase (reSjc26GST)  

Microsoft Academic Search

We have shown previously that immunisation of mice and pigs with recombinant 26 kDa GST (reSjc26GST) induces a pronounced anti-fecundity effect after experimental infection with Chinese Schistosoma japonicum. We report here that anti-fecundity immunity can also be induced against reSjc26GST in Chinese water buffaloes (Bos buffelus), important reservoir hosts for S. japonicum in China. Anti-Sjc26GST antibodies were produced in immunised

Liu Shuxian; He Yongkang; Song Guangchen; Luo Xing-song; Xu Yuxin; Donald P. McManus

1997-01-01

294

Identification of Signatures of Selection in Bos Taurus Beef and Dairy Cattle Using Genome-wide SNP Genotypes  

E-print Network

of the draft Hereford sequence to sequence generated from a series of bacterial artificial chromosomes constructed from Holstein DNA that represented regions from BTA 6, 14, and 25. A pair of samples from 2 outgroups (Bubalus quarlesi and Bubalus bubalis...). Genotypes from 2 individuals from 2 outgroups (Bubalus quarlesi and Bubalus bubalis) were used to assign ancestral allele state using a Perl script. Ancestral allele state was determined for SNP that amplified in Anoa or Water buffalo and was defined...

Choi, Jung Woo

2010-10-12

295

Student Advising Services University at Buffalo  

E-print Network

Student Advising Services University at Buffalo 109 Norton Hall Buffalo, NY 14260 Email: vpue-fif@buffalo.edu Phone: 716-645-6013 Website : http://advising.buffalo.edu/fif/ My Checklist for Staying on Track (approximately 30 credit hours) #12;Student Advising Services University at Buffalo 109 Norton Hall Buffalo, NY

Krovi, Venkat

296

In a buffalo shed  

E-print Network

Sitting in a buffalo shed under a village house, Alan Macfarlane reflects on the origins of settled agriculture and the role of animals and technology. He also considers some of the effects of growing wealth on equality and work....

Macfarlane, Alan

2004-08-26

297

First radiation hybrid map of the river buffalo X chromosome (BBUX) and comparison with BTAX.  

PubMed

We report the first radiation hybrid map of the river buffalo X chromosome generated from a recently constructed river buffalo (Bubalus bubalis) whole-genome radiation hybrid panel (BBURH(5000)). This map contains a total of 33 cattle-derived markers, including 10 genes, four ESTs and 19 microsatellites. The markers are distributed in two linkage groups: LG1 contains eight markers spanning 125.6 cR, and LG2 contains 25 markers spanning 366.3 cR. LG1 contains six markers in common with bovine sequence assembly build 3.1. With the exception of BMS2152, the order of these markers on our BBUX map is shuffled when compared to the cow X chromosome (Bos taurus; BTAX). From LG2, two markers (AMELX and BL22) map to a more distal portion of BTAX compared to BBUX. In addition, two pairs of LG2 markers exhibit inversions compared to BTAX (ILSTS017 and ATRX; XBM38 and PPEF1). Alternatively, when compared to the most recent bovine RH map (Bov-Gen 3000rads), BL1098 and BMS2227 from LG1 as well as PLS3 and BMS1820 from LG2 showed inverted positions on the BBUX map. These discrepancies in buffalo and cattle maps may reflect evolutionary divergence of the chromosomes or mapping errors in one of the two species. Although the set of mapped markers does not cover the entire X chromosome, this map is a starting point for the construction of a high-resolution map, which is necessary for characterization of small rearrangements that might have occurred between the Bubalus bubalis and Bos taurus X chromosomes. PMID:18307583

Ianella, P; Venancio, L P R; Stafuzza, N B; Miziara, M N; Agarwala, R; Schäffer, A A; Riggs, P K; Womack, J E; Amaral, M E J

2008-04-01

298

77 FR 64126 - Notice of Proposed Withdrawal of Public Land for the Buffalo Bill Dam and Reservoir Modification...  

Federal Register 2010, 2011, 2012, 2013

...developed recreation site in the Buffalo Bill Dam and Reservoir Modification...of a Master Plan for the Buffalo Bill State Park and the Buffalo Bill Dam and Reservoir Modification...values that need protection. No water rights would be needed to...

2012-10-18

299

33 CFR 165.911 - Security Zones; Captain of the Port Buffalo Zone.  

Code of Federal Regulations, 2010 CFR

...Navigation and Navigable Waters 2 2010-07-01...Captain of the Port Buffalo Zone. 165.911 ...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...Captain of the Port Buffalo Zone. (a) Location...Plants. The navigable waters of Lake Ontario bounded by the...

2010-07-01

300

33 CFR 165.911 - Security Zones; Captain of the Port Buffalo Zone.  

Code of Federal Regulations, 2011 CFR

...Navigation and Navigable Waters 2 2011-07-01...Captain of the Port Buffalo Zone. 165.911 ...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...Captain of the Port Buffalo Zone. (a) Location...Plants. The navigable waters of Lake Ontario bounded by the...

2011-07-01

301

33 CFR 165.911 - Security Zones; Captain of the Port Buffalo Zone.  

Code of Federal Regulations, 2012 CFR

...Navigation and Navigable Waters 2 2012-07-01...Captain of the Port Buffalo Zone. 165.911 ...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...Captain of the Port Buffalo Zone. (a) Location...Plants. The navigable waters of Lake Ontario bounded by the...

2012-07-01

302

33 CFR 165.911 - Security Zones; Captain of the Port Buffalo Zone.  

...Navigation and Navigable Waters 2 2014-07-01...Captain of the Port Buffalo Zone. 165.911 ...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...Captain of the Port Buffalo Zone. (a) Location...Plants. The navigable waters of Lake Ontario bounded by the...

2014-07-01

303

33 CFR 165.911 - Security Zones; Captain of the Port Buffalo Zone.  

Code of Federal Regulations, 2013 CFR

...Navigation and Navigable Waters 2 2013-07-01...Captain of the Port Buffalo Zone. 165.911 ...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...Captain of the Port Buffalo Zone. (a) Location...Plants. The navigable waters of Lake Ontario bounded by the...

2013-07-01

304

The welfare of dairy buffalo  

Microsoft Academic Search

The present paper addresses the issue of buffalo welfare. Firstly, the biological characteristics and behav - ioural needs of buffalo are considered. Subsequently, the effects of intensive farming and some animal- related indicators, to be used for a monitoring scheme of buffalo welfare at farm level, are described. The attention was focused on the following indicators: excessive thinning or fattening

Giuseppe De Rosa; Fernando Grasso; Corrado Pacelli; Fabio Napolitano; Christoph Winckler

2009-01-01

305

BUFFALO-NIAGARA INTERNATIONAL AIRPORT  

E-print Network

BUFFALO-NIAGARA INTERNATIONAL AIRPORT MICHIGANST. GOODELL ST. WINSPEAR AV KENMORE AV CAMPUS CANADA CITY LIMITS OF BUFFALO VILLAGE OF KENMORE TOWN LIMITS OF AMHERST TOWN OF CHEEKTOWAGA TOWN. SWEETHOMERD. MAPLE RD. N FOREST RD. 90 290 33 62 263 5 5 DOWNTOWN CAMPUS DOWNTOWN BUFFALO TO LOCKPORT

Reineck, James

306

Buffalo Bill Historical Center  

NSDL National Science Digital Library

As a member of the Museums West consortium, the Buffalo Bill Historical Center is itself comprised of five separate museums, including the Buffalo Bill Museum, the Plains Indian Museum, and the Cody Firearms Museum. For those who may not be able to make it to their location in northeastern Wyoming, there are a number of online features that will provide as a suitable substitute for the actual experience of being there. Visitors can feel free to browse the online guide to their research library, and they will most certainly want to look at some of the online exhibits. Some of these online exhibits include features on Buffalo Bill himself, and others (such as those within the Cody Firearms Museum section) feature information on Winchester collectibles and firearms-related sayings.

307

Buffalo Museum of Science  

NSDL National Science Digital Library

If you are heading to Buffalo, you might want to pay a visit to the Buffalo Museum of Science. This website is a great way to learn about the physical museum, including ongoing education programs, research facilities, and various digital collections. In the Exhibits area visitors can explore 12 different ongoing and temporary exhibits, including highlighted exhibits on nanotechnology and mummies of the world. Moving on, the Publications section of the site includes three decades of past Museum publications, including academic works and fact sheets. Users can also learn about visiting the museum in person, complete with information about special hours and special member days.

308

At 1050 Gallery, Block 12, two centrifugal pumps, Buffalo Pumps, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

At 1050 Gallery, Block 12, two centrifugal pumps, Buffalo Pumps, Buffalo, NY, driven by Allis Chalmers motors (size 3 HSO, head 230, 120 cpm, 1750, rpm, Impulse dia. 15) installed in the 1960s and used for water-cooling system for 230-kv cable; the cables have been removed and the pumps are not currently used. - Columbia Basin Project, Grand Coulee Dam & Franklin D. Roosevelt Lake, Across Columbia River, Southeast of Town of Grand Coulee, Grand Coulee, Grant County, WA

309

Bovine calves as ideal bio-indicators for fluoridated drinking water and endemic osteo-dental fluorosis.  

PubMed

Relative susceptibility to fluoride (F) toxicosis in the form of osteo-dental fluorosis was observed in an observational survey of 2,747 mature and 887 immature domestic animals of diverse species living in areas with naturally fluoridated (>1.5 ppm F) drinking water. These animals included buffaloes (Bubalus bubalis), cattle (Bos taurus), camels (Camelus dromedarius), donkeys (Equus asinus), horses (Equus caballus), goats (Capra hircus), and sheep (Ovis aries). Of these mature and immature animals, 899 (32.7 %) and 322 (36.3 %) showed evidence of dental fluorosis with varying grades, respectively. Their incisor teeth were stained with light to deep brownish color. On clinical examination, 31.2 % mature and 10.7 % immature animals revealed periosteal exostoses, intermittent lameness, and stiffness of tendons in the legs as signs of skeletal fluorosis. The maximum susceptibility to fluoride toxicosis was found in bovines (buffaloes and cattle) followed by equines (donkeys and horses), flocks (goats and sheep), and camelids (camels). The bovine calves were found to be more sensitive and highly susceptible to F toxicosis and revealed the maximum prevalence (92.2 %) of dental fluorosis. This indicates that bovine calves are less tolerant and give early sign of F poisoning (dental fluorosis) and therefore, they can be considered as bio-indicators for fluoridated water as well as for endemicity of osteo-dental fluorosis. Causes for variation in susceptibility to F toxicosis (fluorosis) in various species of domestic animal are also discussed. PMID:24671615

Choubisa, S L

2014-07-01

310

33 CFR 162.175 - Black Rock Canal and Lock at Buffalo, New York.  

Code of Federal Regulations, 2011 CFR

... Navigation and Navigable Waters 2 2011-07-01 2011-07-01...Black Rock Canal and Lock at Buffalo, New York. 162.175 ...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...Black Rock Canal and Lock at Buffalo, New York. In the...

2011-07-01

311

33 CFR 162.175 - Black Rock Canal and Lock at Buffalo, New York.  

Code of Federal Regulations, 2012 CFR

... Navigation and Navigable Waters 2 2012-07-01 2012-07-01...Black Rock Canal and Lock at Buffalo, New York. 162.175 ...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...Black Rock Canal and Lock at Buffalo, New York. In the...

2012-07-01

312

33 CFR 162.175 - Black Rock Canal and Lock at Buffalo, New York.  

Code of Federal Regulations, 2013 CFR

... Navigation and Navigable Waters 2 2013-07-01 2013-07-01...Black Rock Canal and Lock at Buffalo, New York. 162.175 ...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...Black Rock Canal and Lock at Buffalo, New York. In the...

2013-07-01

313

33 CFR 162.175 - Black Rock Canal and Lock at Buffalo, New York.  

Code of Federal Regulations, 2010 CFR

... Navigation and Navigable Waters 2 2010-07-01 2010-07-01...Black Rock Canal and Lock at Buffalo, New York. 162.175 ...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...Black Rock Canal and Lock at Buffalo, New York. In the...

2010-07-01

314

33 CFR 162.175 - Black Rock Canal and Lock at Buffalo, New York.  

... Navigation and Navigable Waters 2 2014-07-01 2014-07-01...Black Rock Canal and Lock at Buffalo, New York. 162.175 ...Navigation and Navigable Waters COAST GUARD, DEPARTMENT...Black Rock Canal and Lock at Buffalo, New York. In the...

2014-07-01

315

Water quality in Reedy Fork and Buffalo Creek basins in the Greensboro area, North Carolina, 1986-87  

USGS Publications Warehouse

Water and bottom-sediment samples were collected from April 1986 through September 1987 at 19 sites in Guilford County and the City of Greensboro, North Carolina. Sampling locations included 13 stream sites, two lakes that supply the City of Greensboro with drinking water, two City of Greensboro finished drinking-water filtration plants, and effluent from the two municipal wastewater plants prior to outfall into receiving streams. Water sampling consisted of six surveys during various stages of steady ground-water flow at all sites and high-flow-event sampling during two storms at six sites. Bottom-sediment samples were collected at three sites during two routine sampling surveys. A summary of nearly 22, 000 separate chemical or physical analyses of water samples or bottom sediment is presented and discussed as individual values, ranges of values, or median values with respect to the locations of sampling sites, streamflow conditions, or other information bearing on water-quality conditions under discussion. The results include discussions of general water-quality indicators; major ion, nutrient, and trace-element concentrations; acid and base/neutral extractable organic compounds; volatile organic compounds; and organochlorine and organophosphorus pesticides detected at each sampling site. Loadings of selected constituents are also estimated on a yearly and daily basis. The quality of the raw and finished water, municipal effluents, and streams in the Greensboro area are characterized by using State and Federal water-quality standards. Inorganic constituents most commonly found in excess of standards were iron, copper, zinc, arsenic, phosphorus, manganese, cyanide, and mercury. Relatively few organic compounds were detected; however, those consistently reported were phthalate, thihalomethane, organophosphorus pesticide, benzol, and phenolic compounds. Selected inorganic, physical, and total organic carbon data are used in a Wilcoxon test for two independent variables to statistically compare water-quality characteristics in selected rural, semideveloped and urban basins. During low-flow sampling, the constituents that differed significantly among all sites were calcium, magnesium, and chloride. During low flows, concentrations of orthophosphate, fluoride, sulfate, and TOC differed at the urban site from the rural and semideveloped and urban sites. There were no significant differences among sites in concentrations of sodium, suspended sediment, nickel, zinc, copper, and mercury during low flows. The Wilcoxon test performed on high-flow data indicated that concentrations of TOC, chloride, sulfate, suspended sediment, and nickel were not significantly different among the sites.

Davenport, M.S.

1989-01-01

316

2. Photocopy of photograph (from Buffalo and Erie County Historical ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. Photocopy of photograph (from Buffalo and Erie County Historical Society) Photographer unknown 1928 GENERAL VIEW FROM THE SOUTHWEST - Buffalo Lighthouse, Buffalo Harbor, Buffalo River & Lake Erie, Buffalo, Erie County, NY

317

1. Photocopy of photograph (from the Buffalo and Erie County ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. Photocopy of photograph (from the Buffalo and Erie County Historical Society) Photographer unknown 1859 GENERAL VIEW FROM THE SOUTHEAST - Buffalo Lighthouse, Buffalo Harbor, Buffalo River & Lake Erie, Buffalo, Erie County, NY

318

The bungalows of Buffalo  

Microsoft Academic Search

Measured drawings of seven bungalows that were built between 1914 and 1926 in Buffalo, New York, are presented. The paper concentrates on the conventions which govern the organization of spaces in these bungalows and establish linkages with other house types in the popular tradition. The conventions found are expressed through the schemata of a parametric shape grammar which allow differences

F Downing; U Flemming

1981-01-01

319

Effect of CSN1S1-CSN3 (?(S1)-?-casein) composite genotype on milk production traits and milk coagulation properties in Mediterranean water buffalo.  

PubMed

The aim of this study was to estimate effects of CSN1S1-CSN3 (?(S1)-?-casein) composite genotypes on milk production traits and milk coagulation properties (MCP) in Mediterranean water buffalo. Genotypes at CSN1S1 and CSN3 and coagulation properties [rennet clotting time (RCT), curd firming time (K??), and curd firmness (A??)] were assessed by reversed-phase HPLC and computerized renneting meter analysis, respectively, using single test-day milk samples of 536 animals. Alternative protein variants of ?(S1)-CN and ?-CN were detected by HPLC, and identification of the corresponding genetic variants was carried out by DNA analysis. Two genetic variants were detected at CSN1S1 (A and B variants) and 2 at CSN3 (X1 and X2 variants). Statistical inference was based on a linear model including the CSN1S1-CSN3 composite genotype effect (7 genotypes), the effects of herd-test-day (8 levels), and a combined days in milk (DIM)-parity class. Composite genotype AB-X2X2 was associated with decreased test-day milk yield [-0.21 standard deviation (SD) units of the trait] relative to genotype BB-X2X2. Genotypes did not affect milk protein content, but genotype AB-X1X1 was associated with increased fat content compared with genotype BB-X2X2 (+0.28 SD units of the trait) and AB-X1X1 (+0.43 SD units of the trait). For RCT, the largest difference (+1.91 min; i.e., 0.61 SD units of the trait) was observed between genotype AA-X1X2 and AB-X1X1. Direction of genotype effects on K(20) was consistent with that for RCT. The maximum variation in K?? due to genotype effects (between AA-X1X2 and AB-X1X1 genotypes) was almost 0.9 SD units of the trait. Magnitude of genotype effects was smaller for A?? than for RCT and K??, with a maximum difference of 0.5 SD units of the trait between genotype AA-X1X2 and AA-X1X1. The B allele at CSN1S1 was associated with increased RCT and K?? and with weaker curds compared with allele A. Allele X2 at CSN3 exerted opposite effects on MCP relative to CSN1S1 B. Because of linkage disequilibrium, allele B at CSN1S1 and allele X2 at CSN3 tend to be associated and this likely makes their effects cancel each other. This study indicates a role for casein genes in variation of MCP of buffalo milk. Further studies are necessary to estimate the effects of casein genetic variants on variation of cheese yield. PMID:22612978

Bonfatti, V; Giantin, M; Gervaso, M; Coletta, A; Dacasto, M; Carnier, P

2012-06-01

320

The fishes of Buffalo National River, Arkansas, 2001-2003  

USGS Publications Warehouse

During June through September 2001 and 2002, extensive fish community sampling was conducted at 29 sites within the boundaries of Buffalo National River. Samples were collected using backpack, tote barge, and boat electrofishing equipment. Kick seining also was used at all sites. To supplement these results, samples were collected in 2003 from less typical habitats and during other seasons of the year. Ten supplemental samples were collected from the Buffalo River and five samples were collected from tributaries of the Buffalo River. During the 3 years of sampling, 66 species of fish were collected or observed from the 42 sampling sites. Stonerollers, duskystripe shiners, longear sunfish, and rainbow darters were among the more abundant fish species at most sites. Each of these species is common and abundant throughout much of the Ozark Plateaus in creeks and small rivers. Other species (for example, banded sculpin, southern redbelly dace, orangethroat darter, and Ozark minnow) were among the more abundant species at other sites. These species prefer small- to medium-sized, springfed streams or small creeks. A preliminary list of species expected to occur at Buffalo National River provided by the National Park Service incorrectly listed 47 species because of incorrect species range or habitat requirements. Upon revising this list, the inventory yielded 66 of the 78 species (85 percent). Twelve additional species not collected in 2001-2003 may occur at Buffalo National River for two primary reasons--because the species had been collected previously at the park, or because the park occurs within the known species range and habitats found at the park are suitable for the species. Although no fish species collected from Buffalo National River are federally-listed threatened or endangered species, several species collected at Buffalo National River may be of special interest to National Park Service managers and others. Ten species are endemic to the Ozark Plateaus area and most of these ten are restricted to the White River Basin. For some species the Buffalo River is a population stronghold. The yoke darter and Ozark bass are especially abundant in the Buffalo River. In Arkansas, the Ozark shiner is most abundant in the Buffalo River and, although populations of Ozark shiners are declining in Arkansas, this is not typically the case in the Buffalo River. Data from 2001-2003 indicate that gilt darters currently (2005) are less common in the Buffalo River than during the 1970's. Populations of channel catfish (and any other fish species whose movements are inhibited by the cold water temperatures of the White River) may continue to decline without remedial efforts.

Petersen, James C.; Justus, B.G.

2005-01-01

321

Expression and antigenic characterization of bubaline herpesvirus 1 (BuHV1) glycoprotein E and its potential application in the epidemiology and control of alphaherpesvirus infections in Mediterranean water buffalo.  

PubMed

Bubaline herpesvirus 1 (BuHV1) is a member of ruminant alphaherpesviruses antigenically related to bovine herpesvirus 1 (BoHV1). The impact of BuHV1 infection in infectious bovine rhinotracheitis control program is difficult to establish, due to the lack of specific diagnostic test. The ectodomain of glycoprotein E of BuHV1 was expressed as recombinant secreted protein and used in indirect ELISA as well as in a discriminatory test using the BoHV1 counterpart. A panel of monoclonal antibodies was produced against BuHV1; 6 out of 7 anti-gE monoclonal antibodies specifically recognized the BuHV1 gE. Results indicated BuHV1 gE as a sensitive marker of infection compared to seroneutralization (SN) test or blocking ELISA. When BoHV1 and BuHV1 gEs were immobilized in different wells of the same ELISA microplate, bovine and water buffalo sera were more reactive against the respective infecting virus. About one third of seropositive buffaloes with no history of contact with cattle and having higher SN titres, reacted in BoHV1 gE blocking ELISA, possibly because of steric hindrance. Since in two occasions BuHV1 was also isolated from water buffalo scoring gB+/gE+ BoHV1 blocking ELISA, we conclude that the combination of the two blocking ELISAs is not suitable to differentiate between BoHV1 and BuHV1. PMID:24992670

Nogarol, C; Bertolotti, L; De Carlo, E; Masoero, L; Caruso, C; Profiti, M; Martucciello, A; Galiero, G; Cordioli, P; Lelli, D; Nardelli, S; Ingravalle, F; Rosati, S

2014-10-01

322

33 CFR 207.590 - Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation.  

Code of Federal Regulations, 2013 CFR

...Navigation and Navigable Waters 3 2013-07-01...Rock Canal and Lock at Buffalo, N.Y.; use, administration...Navigation and Navigable Waters CORPS OF ENGINEERS...foot of Bridge Street, Buffalo, N.Y., telephone...under the bridge at low water datum is 17.3...

2013-07-01

323

33 CFR 207.590 - Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation.  

Code of Federal Regulations, 2012 CFR

...Navigation and Navigable Waters 3 2012-07-01...Rock Canal and Lock at Buffalo, N.Y.; use, administration...Navigation and Navigable Waters CORPS OF ENGINEERS...foot of Bridge Street, Buffalo, N.Y., telephone...under the bridge at low water datum is 17.3...

2012-07-01

324

33 CFR 207.590 - Black Rock Canal and Lock at Buffalo, N.Y.; use, administration, and navigation.  

...Navigation and Navigable Waters 3 2014-07-01...Rock Canal and Lock at Buffalo, N.Y.; use, administration...Navigation and Navigable Waters CORPS OF ENGINEERS...foot of Bridge Street, Buffalo, N.Y., telephone...under the bridge at low water datum is 17.3...

2014-07-01

325

PUZZLING POWERS: THE PROBLEM OF FIT NEIL E. WILLIAMS (University at Buffalo)  

E-print Network

PUZZLING POWERS: THE PROBLEM OF FIT NEIL E. WILLIAMS (University at Buffalo) in The Metaphysics' for one another. If the salt is to manifest its solubility in the water, the water must likewise manifest

Williams, Neil E.

326

Evaluation of whole fresh blood and dried blood on filter paper discs in serological tests for Trypanosoma evansi in experimentally infected water buffaloes  

Microsoft Academic Search

In this study we investigated if whole blood could substitute for serum in the direct card agglutination test (CATT\\/Trypansosoma evansi) and the indirect card agglutination test (LATEX\\/T. evansi) for the sero-diagnosis of T. evansi in buffaloes. Likewise blood spots on filter paper were compared with sera for use in the indirect enzyme-linked immunosorbent assay\\/T. evansi (ELISA) and immunotrypanolysis test (T.L.\\/T.

W. G. Holland; N. G. Thanh; L. N. My; E. Magnus; D. Verloo; P. Büscher; B. Goddeeris; J. Vercruysse

2002-01-01

327

Technical and economical feasibility of buffalo gourd as a novel energy crop: Final report  

SciTech Connect

The New Mexico Solar Energy Institute at NMSU has conducted a two-year investigation into the technical and economic feasibility of using the buffalo gourd plant as an energy feedstock in eastern New Mexico. The New Mexico buffalo gourd project conducted field planting trials to determine optimum planting density, fertilizer levels, and irrigation regime. Starchy roots produced by the field plantings were evaluated as an ethanol feedstock at both laboratory and pilot scale. These studies indicate that buffalo gourd is well suited for root production in eastern New Mexico. Current cultivars of buffalo gourd can be most efficiently produced under dry land farming conditions with little, if any, supplemental fertilizer. Traditional plant breeding techniques can be profitably employed on the buffalo gourd to breed a size and shape of root more easily harvested by existing farm machinery. Because of its sensitivity to root rot, buffalo gourd must be grown in well drained soils. Finally, buffalo gourd has been shown to be an excellent feedstock for ethanol production provided necessary pre-fermentation processing (chopping of roots) is performed correctly. A model was created to determine the economic feasibility of growing buffalo gourd in eastern New Mexico. It was determined that the net return to a farmer in eastern New Mexico can be higher planting buffalo gourd than many traditionally grown crops because of buffalo gourd's low water and fertilizer requirements. The model further indicates that net return is heavily influenced by root yield. Continued research is needed to optimize buffalo gourd root yield, as well as root size and shape, disease resistance, etc. A clearly defined R and D agenda and commercialization strategy is presented and discussed. Buffalo gourd has been demonstrated to have high potential as an alternative feedstock for ethanol production in eastern New Mexico. 128 refs., 9 figs., 28 tabs.

Goldstein, B.

1988-02-01

328

Pharmacokinetics and dosage regimen of 2-pyridine aldoxime in Bubalus bubalis intoxicated with fenitrothion.  

PubMed

In the present study, the pharmacokinetics of 2-pyridine aldoxime (2-PAM, 30 mg/kg, i.v.) alone and in conjunction with atropine (0.3 mg/kg; 1/4 i.v., 3/4 i.m.) was investigated in 10 Bubalus bubalis intoxicated with a single oral lethal dose of fenitrothion (435 mg/kg). Based on the kinetic parameters, an appropriate dosage regimen of 2-PAM in B. bubalis was calculated. There was no significant difference between plasma levels and pharmacokinetic parameters of 2-PAM in the two groups of animals, given 2-PAM alone and in conjunction with atropine. The peak plasma concentration of 2-PAM at 1 min was in the range of 189.5-196.6 microg/mL which declined to 9.22-9.98 microg/mL at 4 h. The values of elimination half-life, Vd(area) and total body clearance were 2.41-2.67 h, 0.77-0.95 L/kg and 227.5-245.7 mL/kg/h, respectively. The binding capacity of 2-PAM to plasma proteins of fenitrothion-intoxicated buffalo calves and dissociation rate constant of protein drug complex were 0.015 x 10(-6) mol/g and 2.367 x 10(-6) mol, respectively. Approximately 63% of 2-PAM was bound with plasma proteins. In the treatment of organophosphate insecticide (OPI) toxicity in B. bubalis, an appropriate i.v. dosage regimen of 2-PAM in conjunction with atropine would be 18 mg/kg followed by 15 mg/kg at 4 h interval. PMID:11348483

Srivastava, A K; Malik, J K

2001-02-01

329

Mechanical and Aerospace Engineering University at Buffalo  

E-print Network

Mechanical and Aerospace Engineering University at Buffalo S t a t e U n i v e r s i t y o f N e w Department of Mechanical and Aerospace Engineering University at Buffalo ah@buffalo.edu Abstract: Rotation and Aerospace Engineering of State University of New York at Buffalo. He received his Ph.D. degree

Krovi, Venkat

330

University at Buffalo Empire Innovation Program Initiative  

E-print Network

University at Buffalo Empire Innovation Program Initiative The University at Buffalo (UB), as part of the UB2020 process: Bioinformatics and Life Sciences (http://www.bioinformatics.buffalo.edu/) Molecular Recognition in Biological Systems. (http://www.buffalo.edu/ub2020/academic

Krovi, Venkat

331

Where the Buffalo Roam  

NSDL National Science Digital Library

In this activity, learners explore the Great Plains. They use a large outline map (see related activity "If I Lived in a Forest") to trace the location of the Plains region and examine the types of natural resources available. They play "Name of the Game" as they learn about the importance of the buffalo and other animals to Native Americans of the Plains. This activity is featured on p.21-23 of the "One With the Earth: Native Americans and the Natural World" multidisciplinary unit of study for kindergarten through third grade.

Indianapolis, The C.

2014-05-28

332

33 CFR 110.84 - Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y.  

Code of Federal Regulations, 2012 CFR

... Navigation and Navigable Waters 1 2012-07-01 2012-07-01...opposite foot of Porter Avenue, Buffalo, N.Y. 110.84 Section... Navigation and Navigable Waters COAST GUARD, DEPARTMENT...opposite foot of Porter Avenue, Buffalo, N.Y. An area...

2012-07-01

333

33 CFR 110.84 - Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y.  

... Navigation and Navigable Waters 1 2014-07-01 2014-07-01...opposite foot of Porter Avenue, Buffalo, N.Y. 110.84 Section... Navigation and Navigable Waters COAST GUARD, DEPARTMENT...opposite foot of Porter Avenue, Buffalo, N.Y. An area...

2014-07-01

334

33 CFR 110.84 - Black Rock Channel opposite foot of Porter Avenue, Buffalo, N.Y.  

Code of Federal Regulations, 2013 CFR

... Navigation and Navigable Waters 1 2013-07-01 2013-07-01...opposite foot of Porter Avenue, Buffalo, N.Y. 110.84 Section... Navigation and Navigable Waters COAST GUARD, DEPARTMENT...opposite foot of Porter Avenue, Buffalo, N.Y. An area...

2013-07-01

335

Presence of Campylobacter and Arcobacter species in in-line milk filters of farms authorized to produce and sell raw milk and of a water buffalo dairy farm in Italy.  

PubMed

The objectives of this study were to investigate the presence of Campylobacter spp. and Arcobacter spp. in dairy herds authorized for the production and sale of raw milk and in a water buffalo dairy farm, and to test the antimicrobial susceptibility of the isolates. A total of 196 in-line milk filters were collected from 14 dairy farms (13 bovine and 1 water buffalo) for detection of Campylobacter spp. and Arcobacter spp. by microbiological culture. For each farm investigated, 1 isolate for each Campylobacter and Arcobacter species isolated was tested using the Etest method (AB Biodisk, Solna, Sweden) to evaluate the susceptibility to ciprofloxacin, tetracycline, chloramphenicol, ampicillin, erythromycin, and gentamicin. A total of 52 isolates were detected in 49 milk filters in 12 farms (85.7%) out of 14 and the isolates were identified as Campylobacter jejuni (6), Campylobacter hyointestinalis ssp. hyointestinalis (8), Campylobacter concisus (1), Campylobacter fetus ssp. fetus (1), Arcobacter butzleri (22), and Arcobacter cryaerophilus (14). The small number of isolates tested for antimicrobial susceptibility precludes any epidemiological consideration but highlights that all Campylobacter isolates were susceptible to macrolides, which are the first-choice drugs for the treatment of campylobacteriosis, and that resistance to fluoroquinolones and tetracycline was detected; for Arcobacter isolates, resistance to ampicillin and chloramphenicol was detected. The sale of raw milk for human consumption by self-service automatic vending machines has been allowed in Italy since 2004 and the presence of C. jejuni in in-line milk filters confirms that raw milk consumption is a significant risk factor for human infection. The high occurrence of emerging Campylobacter spp. and Arcobacter spp. discovered in dairy farms authorized for production and sale of raw milk represents an emerging hazard for human health. PMID:23453517

Serraino, A; Florio, D; Giacometti, F; Piva, S; Mion, D; Zanoni, R G

2013-05-01

336

UPPER BUFFALO WILDERNESS AND BUFFALO ADDITION ROADLESS AREA, ARKANSAS.  

USGS Publications Warehouse

The Upper Buffalo Wilderness and Buffalo Addition Roadless Area covers about 19 sq mi in the Ozark National Forest, Newton County, Arkansas. No metal-bearing minerals were observed during geologic mapping, and analyses for zinc and lead contents in surface rock and sediment samples from the study area are not anomalous. Exploratory drilling into the Boone Formation and (or) the Everton Formation will be necessary to evaluate the mineral-resource potential of zinc and lead in the study area.

Miller, Mary H.; Armstrong, Michelle K.

1984-01-01

337

Evaluation of neutron exposure conditions for the Buffalo Reactor  

SciTech Connect

The light water test reactor at the Nuclear Science and Technology Facility of the State University of New York at Buffalo is currently being used to irradiate specimens in in-core positions for NRC-sponsored metallurgical tests. It is important that the neutron exposures for these Buffalo tests be consistent with those determined for related irradiations in the BSR and ORR reactor at ORNL. Therefore, HEDL National Reactor Dosimetry Center dosimetry procedures and ORNL calculational procedures were used for an evaluation of typical test conditions.

Lippincott, E.P.; Kellogg, L.S.; McElroy, W.N.; Baldwin, C.A.

1984-04-01

338

78 FR 41846 - Safety Zones; Annual Fireworks Events in the Captain of the Port Buffalo Zone  

Federal Register 2010, 2011, 2012, 2013

...in the Captain of the Port Buffalo Zone. This action is necessary and intended for the safety of life and property on navigable waters during this event. During each enforcement period, no person or vessel may enter the respective safety...

2013-07-12

339

Page 2 of 3 STATE UNIVERSITY OF NEW YORK AT BUFFALO  

E-print Network

Page 2 of 3 STATE UNIVERSITY OF NEW YORK AT BUFFALO COMPARATIVE MEDICINE AND LABORATORY ANIMAL of animal. b Look for clues in the environment such as blood, diarrhea, vomit, uneaten food or empty water

Krovi, Venkat

340

Comparative studies confirm natural infections of buffaloes by Sarcocystis cruzi  

Technology Transfer Automated Retrieval System (TEKTRAN)

Controversy exists concerning whether cattle and water buffalo sustain infections with cysts distinct arrays species in the genus Sarcocystis. In particular, morphologically similar parasites have been alternately ascribed to S. cruzi or to S. levinei, depending on their occurrence in cattle and wa...

341

Technical and economical feasibility of buffalo gourd as a novel energy crop. Final report, 14 November 1983-31 December 1986  

SciTech Connect

The New Mexico Solar Energy Institute has conducted a two-year investigation into the technical and economic feasibility of using the buffalo gourd plant as an energy feedstock in eastern New Mexico. The studies indicate that buffalo gourd is well suited for root production in eastern NM. Buffalo gourd has been shown to be an excellent feedstock for ethanol production provided necessary pre-fermentation processing (chopping of roots) is performed correctly. A model was created to determine the economic feasibility of growing buffalo gourd in eastern NM. It was determined that the net return to a farmer in eastern NM can be higher planting buffalo gourd than many traditionally grown crops because of buffalo gourd's low water and fertilizer requirements. A clearly defined RandD agenda and commercialization strategy is presented and discussed. Buffalo gourd has been demonstrated to have high potential as an alternative feedstock for ethanol production in eastern NM.

Goldstein, B.

1988-02-01

342

Buffalo river dredging demonstration. Final report  

SciTech Connect

The Corps of Engineers Buffalo District conducted a demonstration of equipment for dredging contaminated sediments. Several thousand cubic yards of sediment were removed from outside the Buffalo River Federal navigation channel limits using three dredge types: (1) open bucket, (2) enclosed bucket, and (3) submersible pump. The effectiveness of a silt screen deployed downstream of the dredge to reduce suspended sediment transport was also evaluated. Extensive sediment and water column monitoring and sampling were conducted during the 2-week demonstration as part of the effort to determine sediment resuspension rates and contaminant releases associated with the dredging operations. Water column samples were analyzed for total suspended solids, total organic carbon, PCBs, PAHs, metals, ammonia, and pH. A water column bioassay test using Daphnia magna was also performed to assess toxicity effects of the dredging operation. Results of this study were used to assess and refine techniques and laboratory tests that have been previously developed by the Corps of Engineers to predict sediment resuspension rates and contaminant releases. In another phase of the study, the Bureau of Mines demonstrated the use of polyelectrolytes for rapid removal of suspended solids from a dilute dredged material slurry.

Averett, D.E.; Zappi, P.A.; Tatem, H.E.; Gibson, A.C.; Tominey, E.A.

1996-02-01

343

Mechanical and Aerospace Engineering University at Buffalo  

E-print Network

Mechanical and Aerospace Engineering University at Buffalo S t a t e U n i v e r s i t y o f N e w at Buffalo Abstract: The goal of anterior cruciate ligament (ACL) reconstruction surgery is to restore normal at the State University of New York at Buffalo. He graduated from Syracuse University with a Ph.D. (2008), M

Krovi, Venkat

344

Mechanical and Aerospace Engineering University at Buffalo  

E-print Network

Mechanical and Aerospace Engineering University at Buffalo S t a t e U n i v e r s i t y o f N e w Y o r k MAE Seminar Series University at Buffalo Nanosatellite Mission: A Space Based Optical of Mechanical and Aerospace Engineering The State University of New York, Buffalo, NY, 14260 Abstract

Krovi, Venkat

345

Mechanical and Aerospace Engineering University at Buffalo  

E-print Network

Mechanical and Aerospace Engineering University at Buffalo S t a t e U n i v e r s i t y o f N e w Engineering Nancy Schiller Engineering Librarian UB University Libraries schiller@buffalo.edu Abstract at Buffalo Libraries with a particular emphasis on the availability and use of electronic research databases

Krovi, Venkat

346

UNIVERSITY AT BUFFALO OFFICE OF ADMISSIONS  

E-print Network

1 of 3 UNIVERSITY AT BUFFALO OFFICE OF ADMISSIONS 12 CAPEN HALL RE-ENTRY APPLICATION or University at Buffalo UB person number ___________________________________ Spring 20________ Fall 20 at the University at Buffalo? Y______ N______ Are you currently enrolled? Yes ______ No ______ Were you previously

Sachs, Frederick

347

New method for the simultaneous identification of cow, sheep, goat, and water buffalo in dairy products by analysis of short species-specific mitochondrial DNA targets.  

PubMed

A novel method is presented here as an analytical tool for food control and authentication of dairy products manufactured from the milk of cow, sheep, goat, and buffalo. The method is based on multiplex polymerase chain reaction (PCR) of species-specific mitochondrial DNA (mtDNA) targets followed by fragment size analysis by capillary electrophoresis. The method includes (a) simultaneous detection of four species, (b) internal control for DNA extraction and PCR, (c) mtDNA as a target for PCR, (d) amplicons of <200 bp, and (e) flexibility in the electrophoresis and fragment size detection method. Species identification proved to be straightforward, efficient, sensitive, and robust. The method is sensitive to an at least 1% (v/v) relative proportion of milk in binary mixtures. A survey of commercial products showed that 12.5% failed to conform to the description of the contents, by either the introduction or absence of listed species, thus demonstrating the relevance of this type of testing. PMID:23025240

Gonçalves, Joana; Pereira, Filipe; Amorim, António; van Asch, Barbara

2012-10-24

348

Return form to: Office of Student Accounts University at Buffalo  

E-print Network

Return form to: Office of Student Accounts University at Buffalo 232 Capen Hall Buffalo, NY 14260 Phone: 716-645-1800 Fax: 716-645-7760 Email: UBstudentaccounts@buffalo.edu Last Updated: 7/2012 Request of Student Accounts University at Buffalo 232 Capen Hall Buffalo, NY 14260 Phone: 716-645-1800 Fax: 716

Krovi, Venkat

349

http://www.cubs.buffalo.edu Feature Selection  

E-print Network

http://www.cubs.buffalo.edu Feature Selection Scatter matrices, Fisher's discriminant, Principal Component Analysis. #12;http://www.cubs.buffalo.edu Feature extraction ­ Structural #12;http://www.cubs.buffalo.edu Feature extraction ­ Structural #12;http://www.cubs.buffalo.edu Feature extraction ­ Texture #12;http://www.cubs.buffalo

Govindaraju, Venu

350

Characteristics and Behavior of a Two-Hour Oscillation in the Buffalo River, Buffalo, New York  

NASA Astrophysics Data System (ADS)

The Buffalo River discharges into Lake Erie near the upper end of the Niagara River. The lower 9.2 km of the river has been designated as a Great Lakes Area of Concern due to environmental problems associated with poor water quality, degraded riparian and river habitat, and contaminated sediments. The U.S. Army Corps of Engineers maintains a navigational channel at a depth of 6.7 m below mean lake level by periodic dredging. In 2011, extensive dredging took place within the upper portions of the river to remove some of the most contaminated sediments. This dredging resulted in both widening and deepening of the channel. The Buffalo River's gradient is low and current velocities generally are <10 cm/sec. The low flow conditions coupled with the orientation of the river allows Lake Erie waters to enter the Buffalo River reversing its flow. The largest episodic lake-driven flow reversals were found during strong westerly wind events that setup an elevated water level at the eastern (Buffalo) end of the lake. Lower amplitude flow reversals could also be associated with subsequent Lake Erie surface seiches or other phenomena. They also occur during times when no seiche conditions are present. The interaction between river flow and reverse (lake-driven) flow was investigated using Acoustic Doppler Current Profilers (ADCPs), temperature sensors, and water level recorders deployed for the past five years at various locations in the lower 9 km of the river. The collected data record the periodic reversals associated with Lake Erie seiches, but also reveal an oscillation within the river. This 'river seiche' has a period of ~2 hours and occurs continuously, persisting even during high flow events and during times of strong lake-driven flow reversals. To better understand the characteristics and behavior of this 'river oscillation', time-series plots and Fourier power spectra were produced from the ADCP data. These data show that the magnitude of the oscillation is on the order of 5-10 cm s-1. There are three coherent spectral peaks with significant power above the noise. These peaks have periods centered on 1.8, 2.0 and 2.2 hours. Our preliminary conjecture is that the oscillation is similar to a forced resonance in a closed basin.

Perez, A. S.; Sabato, J. S.; Singer, J.; Manley, T.

2013-12-01

351

Hydrogeology of the Buffalo aquifer, Clay and Wilkin Counties, West-Central Minnesota  

USGS Publications Warehouse

The Buffalo aquifer is the principal source of ground-water supplies in the Moorhead, Minnesota area. The aquifer is an elongate deposit of sand and gravel, which locally contains water under confined conditions. Although the Buffalo aquifer contains about 270 billion gallons of water in storage, only 120 billion gallons could be withdrawn. Largest well yields occur along the deep trough in the center of the aquifer. Induced streambed infiltration may be possible in certain areas where the stream overlies the aquifer and where the intervening lake sediments are thin or absent. A numerical model constructed for aquifer evaluation has shown that a considerable amount of ground water is discharged through the confining bed to the stream or leaves the area as underflow to the west. Water from the Buffalo aquifer generally is very hard and of the calcium bicarbonate type. The average discharge of the Buffalo River for the base period 1946-78 ranges from 0.229 cubic foot per second per square mile near Hawley to 0.108 cubic foot per second per square mile at Sabin. Surface water in the Buffalo River drainage system is dominantly a calcium bicarbonate type similar to ground water of the area, especially at low flow in the upper reaches of the tributaries. (USGS)

Wolf, R.J.

1981-01-01

352

University at Buffalo (SUNY) Department of Industrial Engineering Scheduling Theory  

E-print Network

University at Buffalo (SUNY) Department of Industrial Engineering IE 661 Scheduling Theory Chapter 2 Rakesh Nagi Department of Industrial Engineering University at Buffalo (SUNY) #12;University at Buffalo (SUNY) Department of Industrial Engineering Chapter 2: Deterministic Models Preliminaries

Nagi, Rakesh

353

University at Buffalo (SUNY) Department of Industrial Engineering Scheduling Theory  

E-print Network

University at Buffalo (SUNY) Department of Industrial Engineering IE 661 Scheduling Theory Chapter 1 Rakesh Nagi Department of Industrial Engineering University at Buffalo (SUNY) #12;University at Buffalo (SUNY) Department of Industrial Engineering Chapter 1: Introduction Role of Scheduling Allocation

Nagi, Rakesh

354

Underway view from starboard side in the Buffalo River with ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Underway view from starboard side in the Buffalo River with grain elevators and lift bridge in background. TC - Fireboat EDWARD M. COTTER, Moored on the Buffalo River at 155 Ohio Street, Buffalo, Erie County, NY

355

University at Buffalo The State University of New York  

E-print Network

University at Buffalo The State University of New York Faculty of the University at Buffalo (UB), The State University of New York, invites applications and reference letters must be submitted online at https://www.ubjobs.buffalo

McCombe, Bruce D.

356

Buffalo News -Buffalo seen as possible link with supercomputer network Subscribe Today -2 weeks FREE  

E-print Network

Buffalo News - Buffalo seen as possible link with supercomputer network Subscribe Today - 2 weeks popular stories More by this author Buffalo seen as possible link with supercomputer network By FRED O Larry Smarr, founder of the National Center for Supercomputing Applications, was the guest speaker

Miller, Russ

357

Chromosome constitution of a hybrid between East African Buffalo (Syncerus caffer caffer) and Dwarf Forest Buffalo  

E-print Network

NOTE Chromosome constitution of a hybrid between East African Buffalo (Syncerus caffer caffer African X Dwarf African Buffalo (Syncerus cafler cagey X Syncerus catfer nanus) and a male Dwarf Forest subspecies of African Buffalo, East African (Syncerus cage7 ca f ey) and Dwarf Forest (Syncevus caffer nanus

Boyer, Edmond

358

Brewster F2A-2 Buffalo  

NASA Technical Reports Server (NTRS)

Brewster F2A-2 Buffalo: The Navy's Brewster F2A-2 Buffalo did not prove to be a particularly effective fighter aircraft, but that did not prevent countries such as England, Finland, and Australia from operating version of the Buffalo. This F2A-2 arrived at Langley from the factory by truck in 1942, and was sent to NAS Norfolk two years later.

1943-01-01

359

Buffalo Bill Letters to George T. Beck  

NSDL National Science Digital Library

Created as part of the larger Rocky Mountain Online Archive, this collection contains a clutch of letters written by William F. "Buffalo Bill" Cody to one George T. Beck. Beck was a Wyoming sheep rancher, oil land developer, and the last president of the Council of the Territory of Wyoming before it became a state in 1890. He was also trained as a mining engineer and was president of the Shoshone Irrigation Company. Most of the letters here concern Cody's interest in this company and its water project. Visitors can read the letters, look over the collection summary, and view a brief biography of Cody and Beck. It's a very interesting look into a lesser-known side of this American icon, one that reveals his intense interest in this rather involved business venture.

2005-01-01

360

Buffalo Trace Oral History Project  

NSDL National Science Digital Library

This engaging project was developed by the Louie B. Nunn Center for Oral History at the University of Kentucky Libraries. It was designed to preserve the story of the Buffalo Trace Distillery in Frankfort, Kentucky which has a truly remarkable history intertwined with that of the Bluegrass State. The project features the people and stories of the Buffalo Trace family, such as master distiller emeritus Elmer T. Lee and descendants of figures like Colonel Albert Blanton and Pappy Van Winkle. On the top of the homepage, visitors can make their way through sections such as Brands, People, Roles, Documentary, and Image Gallery. The Documentary contains a complete film about the history of this unique business and the People section features interesting interviews with those who have made the business known around the world.

361

An Evaluation of the Impact of the Niagara River Ice Boom on the Air Temperature Regime at Buffalo, New York.  

NASA Astrophysics Data System (ADS)

The objective of this study was to determine if the Niagara River ice boom has prolonged the Lake Erie ice cover at Buffalo, New York, resulting in significant changes in the spring warm-up of Lake Erie and longer, colder winters in the area. Statistical analysis of Buffalo air temperatures compared with those for Lockport, NY does not reveal statistically significant cooling in the climate at Buffalo related to the operation of the ice boom. However, because of the distance of the airport (where the temperature gage is located) from the shore zone, the possibility of a localized effect of small magnitude within the vicinity of the ice boom cannot be ruled out. A comparison of the water temperature at the Buffalo intake as recorded in pre- and post-boom years also indicates that the ice boom has not had an impact on the timing of the spring rise in Lake Erie water temperature at Buffalo. Analysis of winter temperature trends since 1898 shows that the winter severity at Buffalo follows a general pattern characteristic not only of the region around the eastern end of Lake Erie but also of the Great Lakes Region as a whole. Winters have become colder since the installation of the ice boom, but these colder winters are part of a general climatic trend toward more severe winters beginning in 1958. Thus, there is no evidence to suggest that the ice boom has increased winter severity or duration at Buffalo relative to other areas around the Great Lakes.

Quinn, Frank H.; Assel, Raymond A.; Gaskill, Daniel W.

1982-03-01

362

In Buffalo, Opening Doors for the Overlooked  

ERIC Educational Resources Information Center

This article describes the Buffalo Prep program. Housed at University of Buffalo, the program identifies disadvantaged but talented minority children, places them in academic-enrichment classes, and then finds them spots at private schools and a more selective public high school in the area to complete their precollegiate careers. In addition to…

Honawar, Vaishali

2007-01-01

363

THE CONFUCIUS INSTITUTE AT THE UNIVERSITY AT BUFFALO  

E-print Network

THE CONFUCIUS INSTITUTE AT THE UNIVERSITY AT BUFFALO Annual Report 2012 2012 The Confucius Institute at the University at Buffalo #12;Contents The Confucius Institute at the University at Buffalo ............................................................................................ 24 #12;The Confucius Institute at the University at Buffalo (UBCI) is dedicated to promoting

McCombe, Bruce D.

364

THE MARKET ARCADE FILM & ARTS CENTER THE BUFFALO FILM SEMINARS  

E-print Network

THE MARKET ARCADE FILM & ARTS CENTER THE BUFFALO FILM SEMINARS SERIES XX/SPRING 2010 CONVERSATIONS Michael Mann, COLLATERAL 2004 THE BUFFALO FILM SEMINARS are presented by University at Buffalo's James, and Humanities Institute, and by the Market Arcade Film & Arts Center, 639 Main Street, Buffalo, N.Y. The series

Krovi, Venkat

365

DIVISION OF COMPARATIVE MEDICINE AND LABORATORY ANIMAL FACILITIES OF SUNY AT BUFFALO  

E-print Network

DIVISION OF COMPARATIVE MEDICINE AND LABORATORY ANIMAL FACILITIES OF SUNY AT BUFFALO STANDARD ANIMAL FACILITIES STANDARD OPERATING PROCEDURE for HUBANDRY OF IMMUNOCOMPROMISED MICE IN THE CMLAF 1 or plastic water bottle fitted with stoppers and stainless sipper tubes filled with tap water and autoclaved

Krovi, Venkat

366

Hydrologic characteristics of Bear Creek near Silver Hill and Buffalo River near St. Joe, Arkansas, 1999-2000  

USGS Publications Warehouse

The Buffalo River and its tributary Bear Creek are in the White River Basin in the Ozark Plateaus in north-central Arkansas. Analysis of streamflow measurements and water-quality samples at a site on Bear Creek and a site on the Buffalo River in Searcy County, Arkansas, quantify differences between the two sites during calendar years 1999 and 2000. Streamflow and water quality also vary seasonally at each site. Mean annual streamflow was substantially larger at the Buffalo River site (836 and 719 cubic feet per second in 1999 and 2000) than at the Bear Creek site (56 and 63 cubic feet per second). However, during times of low flow, discharge of Bear Creek comprises a larger proportion of the flow of the Buffalo River. Concentrations of nutrients, fecal-indicator bacteria, dissolved organic carbon, and suspended sediment generally were greater in samples from Bear Creek than in samples from the Buffalo River. Statistically significant differences were detected in concentrations of nitrite plus nitrate, total nitrogen, dissolved phosphorus, orthophosphorus, total phosphorus, fecal coliform bacteria, and suspended sediment. Loads varied between sites, hydrologic conditions, seasons, and years. Loads were substantially higher for the Buffalo River than for Bear Creek (as would be expected because of the Buffalo?s higher streamflow). Loads contributed by surface runoff usually comprised more than 85 percent of the annual load. Constituent yields (loads divided by drainage area) were much more similar between sites than were loads. Flow-weighted concentrations and dissolved constituent yields generally were greater for Bear Creek than yields for the Buffalo River and flowweighted concentrations yields were higher than typical flow-weighted concentrations and yields in undeveloped basins, but lower than flow-weighted concentrations and yields at a site in a more developed basin.

Petersen, Jim C.; Haggard, Brian E.; Green, W. Reed

2002-01-01

367

Influence of weaning regimen on intake, growth characteristics and plasma blood metabolites in male buffalo calves.  

PubMed

Experiment was conducted to evaluate the effect of weaning age on growth performance, feed intake, feed efficiency (FE) and blood metabolites in Nili-Ravi male buffalo (Bubalus bubalis) calves. Twenty-four male buffalo calves were assigned to one of the three treatment groups: continuous milk feeding (CMF), limited milk feeding (LMF) and early weaning (EW), and weaned off milk at 12, 10 and 8 weeks of age, respectively. For the first 3 days after birth, calves in all three treatments were fed colostrum, and were then moved to individual milk feeding at 10% of BW for the next 6 weeks. Thereafter, the provision of milk to the CMF group was gradually tapered to zero through week 12, using week 6 intakes as a base. The LMF calves were fed milk at 7.5%, 5.0%, 3.5%, and 1.5% of BW during weeks 7 to 10, respectively. Lastly, calves in the EW group were fed milk at 5.0% and 2.5% of BW at weeks 7 and 8, respectively. Calf starter (CS) feed was also provided ad libitum from weeks 2 to 12 and individual intakes were recorded on a daily basis. Blood samples were taken from weeks 6 to 12, on a weekly basis; whereas, the BW, heart girth, withers height and hip width were measured at the start of experiment and later on a weekly basis. Weight gain, average daily gain, and body measurements were the same across all three groups. Milk intake was lower (P < 0.05), whereas CS intake was greater (P < 0.05) in the EW calves compared with the other treatment groups. Dry matter intake was greater (P < 0.05) in the EW and LMF calves compared with the CMF calves. The FE was greater (P < 0.05) in the CMF calves compared with the LMF and EW treatment groups. Blood glucose concentration was similar among the treatments; however, blood urea nitrogen was greater (P < 0.05) in the EW calves compared with the CMF and LMF groups. Plasma concentration of non-esterified fatty acids was higher (P < 0.05) in the EW calves compared with the CMF calves. In light of these results, it is evident that buffalo calves can be successfully weaned as early as 8 weeks of age without negatively affecting their growth performance. PMID:23688620

Rashid, M A; Pasha, T N; Jabbar, M A; Ijaz, A; Rehman, H; Yousaf, M S

2013-09-01

368

PHYLOGEOGRAPHIC PATTERNS IN LARGE RIVER ECOSYSTEMS: GENETIC STRUCTURE OF SMALLMOUTH BUFFALO (ICTIOBUS BUBALUS) IN THE OHIO RIVER  

EPA Science Inventory

Genetic studies on populations of large river fishes provide a potentially useful but underutilized research and assessment tool. Population genetic research on freshwater systems has provided meaningful insight into stock structure, hybridization issues, and gene flow/migration...

369

Isolation of a gene encoding a cellulolytic enzyme from swamp buffalo rumen metagenomes and its cloning and expression in Escherichia coli.  

PubMed

Ruminants are capable of hydrolyzing lignocellulosic residues to absorbable sugars by virtue of the microbial communities residing in their rumen. However, large sections of such microbial communities are not yet culturable using conventional laboratory techniques. Therefore in the present study, the metagenomic DNA of swamp buffalo (Bubalus bubalis) rumen contents was explored using culture-independent techniques. The consensus regions of glycosyl hydrolase 5 (GH5) family of cellulases were used as primers for PCR amplification. A full-length metagenomic cellulase gene, Umcel5B29, with a complete open reading frame (ORF) of 1611 bp was identified. The similarity search analysis revealed that Umcel5B29 is closely related to the cellulases (73% to 98% similarity) of ruminal unculturable microorganisms, indicating its phylogenetic origin. Further analysis indicated that Umcel5B29 does not contain a carbohydrate binding module (CBM). Subsequently, Umcel5B29 was overexpressed in Escherichia coli. The recombinant enzyme worked optimally at pH 5.5 and 45°C, a condition similar to the buffalo's rumen. However, the enzyme retained more than 70% of its maximal activity after incubation at pH 4-7 and more than 50% maximal activity after incubation at 30-60°C for 30 min. These characteristics render Umcel5B29 as a potential candidate for the bio-stoning process of denim. PMID:23134306

Cheema, Tanzeem Akbar; Jirajaroenrat, Kanya; Sirinarumitr, Theerapol; Rakshit, Sudip K

2012-01-01

370

Department of Anthropology University at Buffalo, State University of New York  

E-print Network

Department of Anthropology University at Buffalo, State University of New by October 15, 2013. Applications must be submitted through www.ubjobs.buffalo Committee, Department of Anthropology, University at Buffalo, SUNY, 380 MFAC, Buffalo

McCombe, Bruce D.

371

Lake Erie Seiches and Their Impact on Hydrodynamics and Sediment Transport in the Buffalo River, Buffalo, New York  

NASA Astrophysics Data System (ADS)

The Buffalo River discharges into Lake Erie near the upper end of the Niagara River. The lower 9.2 km of the river has been designated as a Great Lakes Area of Concern due to beneficial use impairments associated with poor water quality, degraded riparian and river habitat, and contaminated sediments. The U.S. Army Corps of Engineers maintains a navigational channel at a depth of 6.7 m below mean lake level by dredging every 2-3 years. Its low gradient and current velocities that often are <10 cm/sec allow Lake Erie waters to enter the Buffalo River reversing flow. These estuarine-like conditions can occur during low flow periods in the river when water levels at the eastern end of Lake Erie rise in response to seiches when persistent winds transport water from west to east increasing water elevation at the Buffalo (eastern) end of the lake. To better understand the interaction between the downriver and upriver (lake-driven) flow, Acoustic Doppler Current Profilers (ADCPs), temperature sensors, and water level recorders have been deployed in the lower 9 km of the river. To map the river and document changes in bottom morphology side-scan sonar surveys have been conducted. Sediment trend analysis (STA) and numerical modeling complement and augment field observations. Changes in grain size distributions derived from the STA show two distinct flow regimes existing in the river with sediments deposited around the mouth of the river re-entrained and transported upriver. Results from numerical modeling using a particle-tracking component also show a similar pattern. Where the two flow regimes intersect, sedimentary furrows have been mapped using side-scan sonar and confirmed by divers. ADCP findings document periodic high flow events in the river related to heavy rainfalls and snow melt as well as the propagation of Lake Erie seiches upriver as far as 9 km. The water level data agree well with ADCP data obtained in the river. The river also exhibits its own seiche with period of ~ 2.0 hours. The hydrodynamics and sediment transport patterns in the Buffalo River reveal a complex pattern. Studying the river is timely as a large-scale sediment remediation project currently is underway that will modify the channel morphology as a consequence of environmental dredging within and outside the navigational channel.

Singer, J.; Manley, T.; McLaren, P.; Manley, P.; Atkinson, J. F.; Hughes, W.; Klawinski, A.

2011-12-01

372

Co-Designing Agents Albert Goldfain ag33@cse.buffalo.edu  

E-print Network

Co-Designing Agents Albert Goldfain ag33@cse.buffalo.edu Michael W. Kandefer mwk3@cse.buffalo.edu Stuart C. Shapiro shapiro@cse.buffalo.edu Department of Computer Science and Engineering, University at Buffalo, Buffalo, NY 14260 Josephine Anstey jranstey@buffalo.edu Department of Media Studies, University

Shapiro, Stuart C.

373

Evaluation of solidification/stabilization technology for Buffalo River sediment. Final report  

SciTech Connect

The Buffalo River drains a 446-square-mile (1,155-sq-km) watershed in western New York State and discharges into Lake Erie at the city of Buffalo. The Buffalo River has been classified by the State of New York as a fishing and fish survival stream, but municipal and industrial discharges have degraded the water quality and resulted in a fish advisory for the river. Under the Assessment and Remediation of Contaminated Sediment Program, the US Environmental Protection Agency asked the US Army Corps of Engineers to evaluate solidification/stabilization (S/S) for potential treatment of the contaminated sediments in the Buffalo River. An evaluation of S/S technology was conducted on the bench-scale level on Buffalo River sediment to determine whether physical and chemical properties of the sediment would be improved. Based on analyses of the untreated sediment, five metals were selected for evaluation: chromium, copper, lead, nickel, and zinc. Initial screening tests (ISTs) were conducted on the sediments to narrow the range of binder-to-soil ratios (BSRs) to be prepared in the detailed evaluation.

Fleming, E.C.; Averett, D.E.; Channell, M.G.; Perry, B.D.

1991-05-01

374

Effect of Ground Corn Cob Replacement for Cassava Chip on Feed Intake, Rumen Fermentation and Urinary Derivatives in Swamp Buffaloes  

PubMed Central

Four Thai - rumen fistulated male swamp buffaloes (Bubalus bubalis), about four years old with 400±20 kg liveweight, were randomly assigned according to a 4×4 Latin square design to receive dietary treatments. The treatments were: ground corn cob (GCC) replacement for cassava chip (CC) in concentrate at 0% (T1); GCC replacement at 33% (T2); GCC replacement at 67% (T3); and GCC replacement at 100% (T4), respectively. During the experiment, concentrate was offered at 0.5% BW while 5% urea-treated rice straw was given at ad libitum. The result revealed that there was no effect of GCC replacement on DMI among treatments. In addition, digestibilities of DM, OM and CP were not different while aNDF linearly increased with an increasing level of GCC replacement. However, GCC replacement did not affect rumen fermentation such as ruminal pH, NH3-N and VFA concentration; except C3 proportion which was the highest at 33% replacement while the lowest was at 100% replacement. All replacements of GCC resulted in similar protozoal and bacterial populations and microbial protein synthesis (MPS). Purine derivatives (PD) concentration in urine and PD to creatinine (PDC) index were varied with time of urination and among treatments at 0 to 8 and 8 to 16 h post feeding and higher values were shown among the GCC replacement groups. However at 16 to 24 h-post feeding, it was untraceable. In addition, creatinine concentration was similar among all treatments at every sampling time. Based on the above results, GCC can be used as an energy source for swamp buffalo fed with rice straw. Spot sampling of urine can be used for purine derivatives determination. PMID:25049671

Wanapat, M.; Pilajun, R.; Kang, S.; Setyaningsih, K.; Setyawan, A. R.

2012-01-01

375

SEROLOGICAL SURVEY OF ANTIBODIES TO TOXOPLASMA GONDII IN SHEEP, CATTLE, AND BUFFALOES IN PUNJAB, INDIA  

Technology Transfer Automated Retrieval System (TEKTRAN)

Sera from 186 sheep, 83 cattle, and 103 water buffaloes from Punjab, India were evaluated for antibodies to Toxoplasma gondii using a commercial ELISA kit. This study was planned using a 2-stage random sampling procedure employing sampling software ‘survey toolbox’. In the first step, villages were...

376

Sequential Cross-Species Chromosome Painting among River Buffalo, Cattle, Sheep and Goat: A Useful Tool for Chromosome Abnormalities Diagnosis within the Family Bovidae  

PubMed Central

The main goal of this study was to develop a comparative multi-colour Zoo-FISH on domestic ruminants metaphases using a combination of whole chromosome and sub-chromosomal painting probes obtained from the river buffalo species (Bubalus bubalis, 2n?=?50,XY). A total of 13 DNA probes were obtained through chromosome microdissection and DOP-PCR amplification, labelled with two fluorochromes and sequentially hybridized on river buffalo, cattle (Bos taurus, 2n?=?60,XY), sheep (Ovis aries, 2n?=?54,XY) and goat (Capra hircus, 2n?=?60,XY) metaphases. The same set of paintings were then hybridized on bovine secondary oocytes to test their potential use for aneuploidy detection during in vitro maturation. FISH showed excellent specificity on metaphases and interphase nuclei of all the investigated species. Eight pairs of chromosomes were simultaneously identified in buffalo, whereas the same set of probes covered 13 out 30 chromosome pairs in the bovine and goat karyotypes and 40% of the sheep karyotype (11 out of 27 chromosome pairs). This result allowed development of the first comparative M-FISH karyotype within the domestic ruminants. The molecular resolution of complex karyotypes by FISH is particularly useful for the small chromosomes, whose similarity in the banding patterns makes their identification very difficult. The M-FISH karyotype also represents a practical tool for structural and numerical chromosome abnormalities diagnosis. In this regard, the successful hybridization on bovine secondary oocytes confirmed the potential use of this set of probes for the simultaneous identification on the same germ cell of 12 chromosome aneuploidies. This is a fundamental result for monitoring the reproductive health of the domestic animals in relation to management errors and/or environmental hazards. PMID:25330006

Pauciullo, Alfredo; Perucatti, Angela; Cosenza, Gianfranco; Iannuzzi, Alessandra; Incarnato, Domenico; Genualdo, Viviana; Di Berardino, Dino; Iannuzzi, Leopoldo

2014-01-01

377

OVERVIEW OF AMERICAN BRASS BUFFALO PLANT FROM ROOF OF STRAND ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

OVERVIEW OF AMERICAN BRASS BUFFALO PLANT FROM ROOF OF STRAND ANNEALING TOWER, INCLUDING CASTING SHOP AND BAG HOUSE (CENTER-LEFT) AND PORTION OF REROLL BAY (R). VIEW LOOKING SOUTHWEST. - American Brass Foundry, 70 Sayre Street, Buffalo, Erie County, NY

378

University at Buffalo (SUNY) Department of Industrial Engineering Scheduling Theory  

E-print Network

1 University at Buffalo (SUNY) Department of Industrial Engineering IE 661 Scheduling Theory Course Introduction Rakesh Nagi Department of Industrial Engineering University at Buffalo (SUNY) #12;2 University at Buffalo (SUNY) Department of Industrial Engineering About the course This graduate level course covers

Nagi, Rakesh

379

RAW COPPER SLABS USED IN CASTING OPERATIONS AT BUFFALO PLANT ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

RAW COPPER SLABS USED IN CASTING OPERATIONS AT BUFFALO PLANT OF AMERICAN BRASS COMPANY. MATERIALS STORAGE FOR THE CAST SHOP NOW OCCUPIES A PORTION OF THE ORIGINAL BRASS MILL BUILT BY THE BUFFALO COPPER AND BRASS ROLLING MILL IN 1906-07 AND EXPANDED IN 1911. - American Brass Foundry, 70 Sayre Street, Buffalo, Erie County, NY

380

7. Concrete Railing along Buffalo River side of tracks emerging ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Concrete Railing along Buffalo River side of tracks emerging from second level of DL&W train shed. Signal Tower/Boiler Room is just out of sight at right of photo. Skyway shows at extreme left. - Delaware, Lackawanna & Western Railroad, Lackawanna Terminal, Main Street & Buffalo River, Buffalo, Erie County, NY

381

THE MARKET ARCADE FILM & ARTS CENTER THE BUFFALO FILM SEMINARS  

E-print Network

THE MARKET ARCADE FILM & ARTS CENTER THE BUFFALO FILM SEMINARS SERIES XXII/SPRING 2011 19 Jafar Panahi DAYEREH/THE CIRCLE 2000 Apr 26 Ridley Scott BLADE RUNNER 1982 THE BUFFALO FILM SEMINARS are presented by University at Buffalo's James Agee Chair in American Culture, Center for Studies

Krovi, Venkat

382

STAGES OF SEMINIFEROUS EPITHELIAL CYCLE IN THE BUFFALO (BOS BUBALIS)  

E-print Network

STAGES OF SEMINIFEROUS EPITHELIAL CYCLE IN THE BUFFALO (BOS BUBALIS) S. S. GURAYA, G. S. BILASPURI) SUMMARY By using PAS-haematoxylin stained preparations of testicular material from mature buffaloes of the stage have been kept in mind. The various stages identified in the buffalo have been compared

Paris-Sud XI, Université de

383

http://www.cubs.buffalo.edu Pattern Recognition  

E-print Network

http://www.cubs.buffalo.edu Pattern Recognition Classification cost Biometric system errors ROC curve #12;http://www.cubs.buffalo.edu Bayesian classification · Bayes classification rule: classify x samples of class 1 2 - the cost of misclassifying samples of class 2 #12;http://www.cubs.buffalo.edu Total

Govindaraju, Venu

384

Carbohydrate biofuels III: Consumptive-use and root yield of buffalo gourd  

SciTech Connect

Biofuel provided by the dried roots of the wild buffalo gourd, Cucurbita foetidissima, represents a potential, cleaner-burning alternative to other biofuels (i.e. wood and coal) currently used for cooking and heating on the Navajo Indian Reservation. However, no information is available regarding the plant`s water requirements for growth and viable root production on the Colorado Plateau in northwestern New Mexico where the Navajo Indian Irrigation Project is located. The primary purpose of this study was to evaluate the relationship between buffalo gourd root production and evapotranspiration under variable irrigation as provided by a line-source design. Total dry root yields ranged from 1.6 Mg ha{sup -1} (5.1 tons/acre), and increased linearly within an irrigation treatment range of 371 to 927 nm (14.6 to 36.5 in.), respectively. Peak average daily water-use of buffalo gourd providing maximum root yield was 8.6 mm (0.34 in.) and occurred in late July to early August. Results of this study indicate that buffalo gourd can be successfully grown in northwestern New Mexico when irrigated. Other observations during this study suggest that planting rates for optimum root production need to be established.

Smeal, D.; Gregory, E.J.; Tomko, J. [New Mexico State Univ., Farmington, NM (United States)] [and others

1995-11-01

385

Buffalo Architecture Foundation Building Stories Collection  

NSDL National Science Digital Library

The University of Buffalo has periodically published some great guides to the architecture of its corner of western New York. The University Libraries have digitized all 21 of these fine pamphlets and placed them online for public consumption. Visitors can search for items of note via the Advanced Search tab, or they can just click on the ones that look interesting. The "A Building Like A Superhero" piece is a great place to start. It profiles the massive modernist Bulger Communication Center structure on the campus of Buffalo State College. Moving on, "Buffalo Underground: An Excursion into the Depths of Scajaquada Creek" takes interested parties into the world of this most unusual culvert and its windy ways.

386

76 FR 60962 - Noise Exposure Map Update for Buffalo Niagara International Airport, Buffalo, NY  

Federal Register 2010, 2011, 2012, 2013

...Aviation Administration Noise Exposure Map Update for Buffalo Niagara International...determination that the updated noise exposure maps submitted by the Niagara Frontier Transportation...FAA's determination on the noise exposure maps is September 20, 2011. FOR FURTHER...

2011-09-30

387

This article was downloaded by:[University at Buffalo (SUNY)] On: 27 May 2008  

E-print Network

This article was downloaded by:[University at Buffalo (SUNY)] On: 27 May 2008 Access Details of Buffalo-SUNY, Buffalo, NY, USA b NYS Center for Engineering Design and Industrial Innovation, University of Buffalo, Buffalo, NY, USA First Published on: 21 May 2008 To cite this Article: Dolan, Bryan and Lewis

Lewis, Kemper E.

388

Grid Computing in Buffalo, New York Mark L. Green* and Russ Miller**  

E-print Network

Grid Computing in Buffalo, New York Mark L. Green* and Russ Miller** * Center for Computational Research, University at Buffalo, 9 Norton Hall, Buffalo, New York 14260; Civil, Structural and Environmental Engineering, University at Buffalo, 240 Ketter Hall, Buffalo, New York 14260, (716) 645-6500 x522

Miller, Russ

389

NEIL E. WILLIAMS curriculum vitae jan 1 2010 Department of Philosophy email: new@buffalo.edu  

E-print Network

NEIL E. WILLIAMS curriculum vitae jan 1 2010 Department of Philosophy email: new@buffalo.edu University at Buffalo cell: (716) xxx-xxxx 135 Park Hall work: (716) 645-0161 Buffalo, NY 14260 web: www.acsu.buffalo APPOINTMENTS Assistant Professor ­ University at Buffalo (SUNY) Philosophy 2005 - present PUBLICATIONS ADVISORY

Williams, Neil E.

390

NEIL E. WILLIAMS curriculum vitae nov 11 2010 Department of Philosophy email: new@buffalo.edu  

E-print Network

NEIL E. WILLIAMS curriculum vitae nov 11 2010 Department of Philosophy email: new@buffalo.edu University at Buffalo cell: (716) xxx-xxxx 135 Park Hall work: (716) 645-0161 Buffalo, NY 14260 web: www.acsu.buffalo ­ University at Buffalo (SUNY) Philosophy 2005 - present PUBLICATIONS (Refereed except where noted) EDITOR

Williams, Neil E.

391

Description of Events Where African Buffaloes (Syncerus caffer) Strayed from the Endemic Foot-and-Mouth Disease Zone in South Africa, 1998-2008.  

PubMed

African buffaloes (Syncerus caffer) are reservoir hosts of Southern African Territories (SAT) foot-and-mouth disease (FMD) virus strains. In South Africa, infected buffaloes are found in the FMD-infected zone comprising the Kruger National Park (KNP) and its adjoining reserves. When these buffaloes stray into livestock areas, they pose a risk of FMD transmission to livestock. We assessed 645 records of stray buffalo events (3124 animals) from the FMD infected zone during 1998-2008 for (i) their temporal distribution, (ii) group size, (iii) age and gender composition, (iv) distance from the infected zone fence and (v) outcome reported for each event. A maximum entropy model was developed to evaluate spatial predictors of stray buffalo events and assess current disease control zones. Out of all buffaloes recorded straying, 38.5% escaped from the FMD infected zone during 2000/2001, following floods that caused extensive damage to wildlife fences. Escape patterns were not apparently influenced by season. The median size of stray groups was a single animal (IQR [1-2]). Adult animals predominated, comprising 90.4% (620/686) of the animals for which age was recorded. Of the 315 events with accurate spatial information, 204 (64.8%) were recorded within 1 km from the FMD infected zone. During late winter/spring (June-October), stray buffaloes were found significantly closer to the FMD infected zone (median = 0.3 km, IQR [0.1-0.6]). Less than 13% (40/315) of stray groups reached the FMD protection zone without vaccination, posing a higher risk of spreading FMD to these more susceptible livestock. Model outputs suggest that distance from the FMD infected zone, urban areas and permanent water sources contributed almost 85% to the spatial probability of stray buffalo events. Areas with a high probability for stray buffalo events were well covered by current disease control zones, although FMD risk mitigation could be improved by expanding the vaccination zone in certain areas. PMID:25377758

van Schalkwyk, O L; Knobel, D L; De Clercq, E M; De Pus, C; Hendrickx, G; Van den Bossche, P

2014-11-01

392

Evaluation of the effectiveness of using alfalfa and buffalo grass for remediation of trichloroethylene from groundwater  

SciTech Connect

Phytoremediation is receiving increasing attention due to the potential for vegetation to play a significant role in bioremediation of contaminated soils and groundwater. The purpose of this research was to conduct a pilot study to determine if buffalo grass would enhance the remediation of groundwater contaminated with trichloroethylene (TCE). A mass-balance experiment was designed and executed to determine the extent of TCE remediation/degradation occurring through buffalo grass. Measurements for TCE in air, water, and soil were completed for three treatments: (1) buffalo grass, (2) alfalfa, and (3) soil following challenge with a water-TCE mixture. In total, 267 air samples, 43 water samples, 85 soil samples, and 40 vegetative samples were collected and analyzed. The analysis identified two important facts. First, there were no significant differences detected between TCE concentrations in soil, water, and air between groups. Second, there is a significant difference in the amount of the TCE-water mixture consumed in chambers with plants versus chambers without plants. The mass balance of the experiment was not achieved due to unaccountable losses of TCE from the chambers. The major loss mechanism for TCE appears to be from the breakthrough of air sampling media during the experiment. Thus, the data are insufficient to determine if remediation occurred via plants or by preferential pathways through the soil. Future experiments should be designed to include daily monitoring of the aquifer, humidity tolerant air sampling protocol, and relief from the build-up of humidity and transpiration inside the chambers.

Caravello, V.

1998-06-03

393

Snow From Great Lakes Covers Buffalo  

NASA Technical Reports Server (NTRS)

On November 20, 2000, Buffalo, New York was blanketed by a late-autumn storm that left 25 inches of snow on the ground in a 24-hour period, most of it during the afternoon rush hour. Buffalo officials declared a state of emergency and New York National Guardsmen were called in to assist with clearing snow from roads. With the exception of essential vehicles or people retrieving stranded children, all driving was banned in the city. This SeaWiFS pass over the central United States and Canada depicts a source for all of the snow in Buffalo. Cold, dry Canadian air blowing toward the southeast picked up a lot of moisture from the relatively warm Great Lakes -- forming the clouds that lightened their loads over Buffalo. This image was acquired November 21, 2000, by the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) flying aboard the Orbview-2 satellite. Image provided by the SeaWiFS Project, NASA/Goddard Space Flight Center, and ORBIMAGE

2002-01-01

394

Parochial School Foundations of Buffalo's Polonia.  

ERIC Educational Resources Information Center

Focuses on the parochial school as a supportive agent of ethnic socialization in Buffalo's Polish immigrant communities. Describes diverse motives for development of the parish school system as an alternative to the public schools and summarizes the distinctive, communal functions of parish schools. (CMG)

Obidinski, Eugene

1984-01-01

395

Poetry at Buffalo: The Twentieth Century.  

ERIC Educational Resources Information Center

Discusses the Special Collections at the State University of New York at Buffalo and the role of Charles Abbott in their development. His interest and a serendipitous lack of funds have created an unusual collection containing a Twentieth Century Poetry Collection, James Joyce holographs, and rare book collections. (CHC)

Knoer, Wanda

1981-01-01

396

36. Photocopy of photograph (Buffalo CourierExpress article file #Z733B9268, print ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

36. Photocopy of photograph (Buffalo Courier-Express article file #Z733-B9268, print in possession of Ciminilli Construction, Buffalo, N.Y.), photographer unknown, 1938 GENERAL RENOVATIONS - Cyclorama Building, 369 Franklin Street, Buffalo, Erie County, NY

397

30. Photocopy of photograph (from Buffalo Illustrated file #129B8B92, prints ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

30. Photocopy of photograph (from Buffalo Illustrated file #129B8B92, prints inpossession of Ciminilli Construction,Buffalo, N.Y.), photographer unknown,1890 GENERAL VIEW LOOKING WEST - Cyclorama Building, 369 Franklin Street, Buffalo, Erie County, NY

398

78 FR 26416 - Environmental Impact Statement: City of Buffalo, Erie County, New York  

Federal Register 2010, 2011, 2012, 2013

...Environmental Impact Statement: City of Buffalo, Erie County, New York AGENCY: Federal...proposed highway project in the City of Buffalo, Erie County, New York. FOR FURTHER...NYSDOT Region 5; 100 Seneca Street, Buffalo, NY 14203, Telephone (716)...

2013-05-06

399

STATE UNIVERSITY OF NEW YORK AT BUFFALO DEPARTMENT OF MECHANICAL AND AEROSPACE ENGINEERING  

E-print Network

STATE UNIVERSITY OF NEW YORK AT BUFFALO DEPARTMENT OF MECHANICAL AND AEROSPACE ENGINEERING GRADUATE AND AEROSPACE ENGINEERING STATE UNIVERSITY OF NEW YORK AT BUFFALO 318 JARVIS HALL BUFFALO, NY 14260-4400 WAIVER

Krovi, Venkat

400

77 FR 20871 - Region II Buffalo District Advisory Council; Public Meeting  

Federal Register 2010, 2011, 2012, 2013

...SMALL BUSINESS ADMINISTRATION Region II Buffalo District Advisory Council; Public Meeting...for the next meeting of the Region II Buffalo District Advisory Council. The meeting...announces the meeting of the Region II Buffalo District Advisory Council. The...

2012-04-06

401

An aerosol-mediated magnetic colloid: Study of nickel nanoparticles Department of Chemistry, The University at Buffalo, The State University of New York, Buffalo,  

E-print Network

of Chemistry, The University at Buffalo, The State University of New York, Buffalo, New York 14260 and Institute for Lasers, Photonics and Biophotonics, The University at Buffalo, The State University of New York, Buffalo, New York 14260 Y. He and M. T. Swihart Department of Chemical and Biological Engineering

Swihart, Mark T.

402

The flood of December 1982 and the 100- and 500-year flood on the Buffalo River, Arkansas  

USGS Publications Warehouse

Flood profiles, peak discharges, and stages were determined for the December 1982, the 100-year, and the 500-year floods at 17 sites along the Buffalo River, Arkansas. Typical synthetic stage hydrographs for the 100- and 500-year floods were determined for each site. Flow duration data for gaging stations at St. Joe and Rush are shown. The average velocity of the water for the 100- and 500-year floods is shown for each site. Approximate flood boundaries delineating the 100- and 500-year floods are shown for Ponca, Steel Creek, Pruitt, St. Joe, and Buffalo Point. (Author 's abstract)

Neely, B.L.

1985-01-01

403

ANALYSIS OF BANK STABILITY AND POTENTIAL LOADINGS FROM STREAMBANKS ALONG THE SOUTH BRANCH OF THE BUFFALO RIVER, MN  

Technology Transfer Automated Retrieval System (TEKTRAN)

The South Branch of the Buffalo River is part of the larger Red River Basin, MN. In 1996 the Minnesota Pollution Control Agency (MPCA) performed water quality assessments for selected rivers and lakes in the Red River Basin, with impairment of streams primarily being found to be caused by high level...

404

Quality of cooked ground buffalo meat treated with the crude extracts of Moringa oleifera (Lam.) leaves  

Microsoft Academic Search

The study was conducted to evaluate the physico-chemical, microbial and organoleptic qualities of cooked ground buffalo meat\\u000a (GBM), treated with, 1, 1.5 and 2% levels of aqueous solution of crude extract of drumstick (Moringa oleifera) leaves. The\\u000a meat samples treated with 1.5% crude extract of drumstick leaves significantly (P?water holding capacity (WHC) and lowered cooking

Suchandra Hazra; Subhasish Biswas; Debasish Bhattacharyya; Sudip Kumar Das; Anupam Khan

405

Buffalo district heating system design and construction  

SciTech Connect

This report addresses the introduction of district heating in Buffalo, NY from feasibility study to implementation. The reemergence of district heating in the US and associated advantages are reviewed. Advanced piping technology which has enabled district heating to compete economically with alternative technologies is summarized. Identification and analysis of the customer heat load considered in downtown Buffalo for the pilot system and future expansion is discussed. Various options for initiating construction of a district heating system were considered as exemplified by the configuration for the pilot system which was selected to serve five downtown buildings. A conceptual plan is presented which permits the system to expand in an economically viable manner. The report concludes with an economic analysis which simulates the operation and expansion of the system. 4 figs., 8 tabs.

Oliker, I.

1987-11-01

406

APPLIED INVESTIGATIONS ON OVARIAN INACTIVITY IN BUFFALO% HEIFERS  

Microsoft Academic Search

Ovarian inactivity hinders the productive and reproductive performances of buffalo and it causes great economic losses, especially in small holder farms, whereas animals are exposed to a lot of stressful conditions. A total number of 372 heads of buffalo%heifers, 2.5 years o ld at least, kept at small holder farms at villages of Lower Egypt was examined during the breeding

W. M. Ahmed; H. H. ElKhadrawy; H. A. Amer

2010-01-01

407

"Just Following the Buffalo": Origins of a Montana Metis Community  

ERIC Educational Resources Information Center

By 1879 the vast buffalo herds were all but gone from the Great Plains. Many of the remaining animals had moved south from the Milk River of northern Montana and Alberta into the Judith Basin of central Montana. In these rich grasslands, for a few more years, life went on as it had for centuries. Following the buffalo came many Indian bands, as…

Foster, Martha Harroun

2006-01-01

408

PARALYTIC DISORDERS ASSOCIATED WITH PHOSPHORUS DEFICIENCY IN BUFFALOES  

Microsoft Academic Search

An investigation was carried out during the month of May to August 2001 to diagnose hind limbs paralysis in buffaloes of Buner area of NWFP. Serum concentrations of calcium (Ca) and phosphorus (P) in 40 buffaloes (29 affected and 11 normal) from six different villages were measured together with feed and soil samples analysed for different macro and micro minerals.

G. Habib; G. Jabbar; M. M. Siddiqui; Z. Shah

2004-01-01

409

Buffalo Flat Service 115-KV Transmission Project : Environmental Assessment.  

SciTech Connect

The US Air Force has sited a radar transmitter at Buffalo Flat, near Christmas Valley, Oregon. This report discusses the environmental impacts of providing the electrical service for the installation. A 115 kV power transmission line will be built between LaPine and Buffalo Flat. Route alternatives as well as design alternatives are discussed. (ACR)

United States. Bonneville Power Administration.

1987-07-01

410

THE MARKET ARCADE FILM & ARTS CENTER THE BUFFALO FILM SEMINARS  

E-print Network

THE MARKET ARCADE FILM & ARTS CENTER THE BUFFALO FILM SEMINARS SERIES XXIII/FALL 2011 CONVERSATIONS OF A COUNTRY PRIEST/JOURNAL D'UN CURÃ? DE CAMPAGNE Robert Bresson 1951 October 4 BLACK ORPHEUS/ORFEU NEGRO, and by the Market Arcade Film & Arts Center, 639 Main Street, Buffalo, N.Y. The series has been supported

Krovi, Venkat

411

Tracing movement of African buffalo in southern Africa  

Microsoft Academic Search

Summary Genetic characterisation of two pathogens, namely foot and mouth disease (FMD) virus and Mycobacterium bovis, isolated from African buffalo (Syncerus caffer) in southern Africa was used to determine the origin of buffalo in situations where the source of infection was obscure. By determining the phylogenetic relatedness of various FMD virus isolates using partial sequencing of the main antigenic determinant,

W. Vosloo; A. D. S. Bastos; A. Michel; G. R. Thomson

2001-01-01

412

The reproductive pattern and efficiency of female buffaloes  

Microsoft Academic Search

Buffaloes play a prominent role in rural livestock production, particularly in Asia. Reproductive efficiency is the primary factor affecting productivity and is hampered in female buffalo by (i) inherent late maturity, (ii) poor estrus expression in summer, (iii) distinct seasonal reproductive patterns, and (iv) prolonged intercalving intervals. Ovarian function is central to these issues; hence, the focal point of this

J. Singh; A. S. Nanda; G. P. Adams

2000-01-01

413

EVALUATION OF POPULATION EFFECTS OF BOVINE TUBERCULOSIS IN FREE-RANGING AFRICAN BUFFALO ( SYNCERUS CAFFER )  

Microsoft Academic Search

We examined data from 3,743 buffalo (Syncerus caffer) culled between 1991 and 1998 in Kruger National Park, South Africa, to evaluate effects of bovine tuberculosis on the buffalo population and examine risk factors of bovine tuberculosis. We found no evidence that bovine tuberculosis affected fertility or lactation status of female buffalo, but adult buffalo .3 years old were underrepresented in

Timothy C. Rodwell; Ian J. Whyte; Walter M. Boyce

2001-01-01

414

SOME BEHAVIORAL ASPECTS OF FOREST BUFFALO (SYNCERUS CAFFER NANUS): FROM HERD TO  

E-print Network

nanus) is 1 of the 3 recognized subspecies of African buffalo (Blancou 1935; Haltenorth and Diller 1979 African buffalo (Syncerus caffer brachyceros). These 2 latter buffalos inhabit African savannahs, whereas subspecies is the Cape buffalo of eastern and southern African savannahs, for which detailed descrip- tions

Penteriani, Vincenzo

415

Selection at the Y Chromosome of the African Buffalo Driven by Rainfall  

E-print Network

Selection at the Y Chromosome of the African Buffalo Driven by Rainfall Pim van Hooft1,2 *, Barend AE, et al (2007) Selection at the Y Chromosome of the African Buffalo Driven by Rainfall. PLoS ONE 2 of selection in the African buffalo (Syncerus caffer). African buffalo are polygamous and live in herds of up

Getz, Wayne M.

416

Habitat preferences of the secretive forest buffalo (Syncerus caffer nanus) in Central Africa  

E-print Network

Syncerus caffer nanus is one of the three subspecies of African buffalo inhabiting the rainforests recognized subspecies of African buffalo (Blancou, 1935; Sinclair, 1977; Haltenorth & Diller, 1979), the other sub- species being the Cape buffalo Syncerus caffer caffer and the Western African buffalo

Penteriani, Vincenzo

417

University at Buffalo The State University of New York Interactive Exploration of  

E-print Network

University at Buffalo The State University of New York Interactive Exploration of Coherent Patterns University at Buffalo University at Buffalo The State University of New York 08.25.03 #12;University at Buffalo The State University of New York Microarray Technology http://www.ipam.ucla.edu/programs/fg2000

Pei, Jian

418

Cytogenetic studies of buffaloes in Romania Brndu&jadnr;a Elena LIVESCU  

E-print Network

Cytogenetic studies of buffaloes in Romania Brându&jadnr;a Elena LIVESCU Genetics Department, Research Institute for Cattle Bredding 8113 Corbeanca, Romania The karyotype of two buffalo populations chromosomes. Our results support the view that the buffaloes from Romania belong to the same Asiatic buffalo

Paris-Sud XI, Université de

419

Effect of Stream Impoundment in Tennessee on the Fish Populations of Tributary Streams  

Microsoft Academic Search

Data from a statewide survey of warm water streams in Tennessee were used to determine the extent to which gizzard shad (Dorosoma cepedianum), carp (Cyprinus carpio), smallmouth buffalo (Ictiobus bubalus), and drum (Aplodinotus grunniens) inhabit smallmouth bass-rock bass streams. Reproduction of those species in tributary streams was minor or absent. By comparing samples from streams that were accessible from an

C. E. Ruhr

1957-01-01

420

Interspecific Comparisons and the Potential Importance of Nutrient Excretion by Benthic Fishes in a Large Reservoir  

Microsoft Academic Search

Fishes can provide an important link between benthic and pelagic habitats by removing nutrients from sediments and excreting them into the water column. The relative importance of nutrients excreted by fishes to ecosystem productivity may vary among species and with abiotic conditions. I measured excretion rates of three benthic feeding fishes, gizzard shad Dorosoma cepedianum, smallmouth buffalo Ictiobus bubalus, and

Keith B. Gido

2002-01-01

421

National Science Foundation boosts area research -2005-11-16 http://buffalo.bizjournals.com/buffalo/stories/2005/11/14/daily22.html 1 of 2 12/1/2005 3:05 PM  

E-print Network

National Science Foundation boosts area research - 2005-11-16 http://buffalo.bizjournals.com/buffaloNews by Markets More News and FeaturesMore News and Features Subscribe to Business First of Buffalo Home » Buffalo at Buffalo will share in an $800,000 National Science Foundation award to establish a computational grid

Miller, Russ

422

Agricultural Energy Conservation Project. Buffalo County target area. Progress report, 1984  

SciTech Connect

As part of the Agriculture Energy Conservation Project, an irrigation management demonstration program was begun in Buffalo County. Irrigation scheduling, furrow irrigation management, center pivot management, and interrow tillage practices were demonstrated on five fields in the county. This project demonstrates that irrigation scheduling (the proper timing of irrigations and the proper application amount) and pumping plant testing are important to reduce the amount of energy and water consumed by irrigation. Proper distribution of water throughout the field is also an important consideration regardless of the type of system being used.

Bockstadter, T.L.; Eisenhauer, D.E.

1985-01-01

423

Habitat Suitability Index Models: Bigmouth Buffalo  

USGS Publications Warehouse

A review and synthesis of existing information were used to develop riverine and lacustrine habitat models for Bigmouth buffalo (Ictiobus cyprinellus), a freshwater fish. The models are scaled to produce an indices of habitat suitability between 0 (unsuitable habitat) and 1 (optimally suitable habitat) for freshwater areas of the continental United States. Other habitat suitability models found in the literature are also included. Habitat suitability indices (HSI's) are designed for use with the habitat evaluation procedures developed by the U.S. Fish and Wildlife Service.

Edwards, Elizabeth A.

1983-01-01

424

University of Buffalo Teaching & Learning Center  

NSDL National Science Digital Library

For folks studying evolutionary biology, this collection from the University of Buffalo's Teaching and Learning Center is a gem. The site contains materials that cover sixteen different animals in a form that resembles a digital image narrative. Visitors can view multiple, fully labeled external and internal views and details of each animal. These materials were developed by Professor Clyde F. Herreid and a team of librarians, graduate student assistants and other staff. Users can browse around by subject heading (such as grasshoppers) or they can also search the entire collection. Each specimen is very well marked and it's a great way to learn about the world of evolutionary biology.

425

PLEASE SCROLL DOWN FOR ARTICLE This article was downloaded by: [University at Buffalo, the State University of New York (SUNY)  

E-print Network

PLEASE SCROLL DOWN FOR ARTICLE This article was downloaded by: [University at Buffalo, the State, State University of New York (SUNY)-Buffalo, Buffalo, New York b Research Institute on Addictions, State University of New York (SUNY)-Buffalo, Buffalo, New York Online publication date: 20 August 2010 To cite

Wu, Changxu (Sean)

426

Buffaloed by the energy boom  

SciTech Connect

The western energy boom is producing the mineral wealth to restore the US economy and security as well as producing boom towns that should be getting more media and public attention. Accurate and more sensitive journalism is needed to record what is happening in the Rocky Mountain region beyond the headlines of confrontations and boosterism. The case of uranium mining and milling is one of a long cycle of land acquisition and development, but it produced little historical record until incidents happened and charges of negligence were made. There is no shortage of material for reporters who want to cover the story of strip mining, water resources, and other issues, but the public must appreciate the life cycle of a mine before it can fully grasp the significance of individual events. This requires aggressive and independent news gathering and sustained coverage. (DCK)

Morris, R.

1981-11-01

427

WEST ELEVATION OF USAIR MAINTENANCE HANGAR AT GREATER BUFFALO INTERNATIONAL ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

WEST ELEVATION OF USAIR MAINTENANCE HANGAR AT GREATER BUFFALO INTERNATIONAL AIRPORT. A BOEING 737-200 HAS BEEN TOWED IN FOR AN OVERNIGHT (BALANCE) CHECK. THE TAIL DOCK STANDS ARE IN POSITION AT THE REAR OF THE AIRCRAFT TO FACILITATE INSPECTION. MAINTENANCE CREWS PERFORM NIGHTLY SERVICE ON UP TO 6 AIRCRAFT. THE NORMAL SEQUENCE OF 12 ROUTINE CHECKS COVERS SEVEN BASIC AREAS: INTERIOR, EXTERIOR, WINGS, LANDING GEAR, TAIL, AUXILIARY POWER UNIT (APU), AND ENGINES. THE WORK FORCE CONSISTS OF 5 INSPECTORS, 3 LEAD MECHANICS, AND 24 MECHANICS; NIGHTLY SCHEDULES ARE COORDINATED BY A PLANNER. - Greater Buffalo International Airport, Maintenance Hangar, Buffalo, Erie County, NY

428

Phylogeography of the African buffalo based on mitochondrial and Y-chromosomal loci: Pleistocene origin and population expansion of the Cape buffalo subspecies  

Microsoft Academic Search

Population genetics and phylogeography of the African buffalo ( Syncerus caffer ) are inferred from genetic diversity at mitochondrial D-loop hypervariable region I sequences and a Y-chromosomal microsatellite. Three buffalo subspecies from different parts of Africa are included. Nucleotide diversity of the subspecies Cape buffalo at hypervariable region I is high, with little differentiation between populations. A mutation rate of

Hooft van W. F; A. F. Groen; H. H. T. Prins

2002-01-01

429

Genetic Variation and Phylogenetic Relationships of Indian Buffaloes of Uttar Pradesh  

PubMed Central

India possesses a total buffalo population of 105 million out of which 26.1% inhabit Uttar Pradesh. The buffalo of Uttar Pradesh are described as nondescript or local buffaloes. Currently, there is no report about the genetic diversity, phylogenetic relationship and matrilineal genetic structure of these buffaloes. To determine the origin and genetic diversity of UP buffaloes, we sequenced and analysed the mitochondrial DNA D-loop sequences in 259 samples from entire Uttar Pradesh. One hundred nine haplotypes were identified in UP buffaloes that were defined by 96 polymorphic sites. We implemented neutrality tests to assess signatures of recent historical demographic events like Tajima’s D test and Fu’s Fs test. The phylogenetic studies revealed that there was no geographic differentiation and UP buffaloes had a single maternal lineage while buffaloes of Eastern UP were distinctive from rest of the UP buffaloes. PMID:25049904

Joshi, Jyoti; Salar, R. K.; Banerjee, Priyanka; S, Upasna; Tantia, M. S.; Vijh, R. K.

2013-01-01

430

EFFECT OF RUST ON THE WETTABILITY OF STEEL BY WATER W. Lu and D.D.L. Chung1  

E-print Network

EFFECT OF RUST ON THE WETTABILITY OF STEEL BY WATER W. Lu and D.D.L. Chung1 Composite Materials Research Laboratory, State University of New York at Buffalo, Buffalo, NY 14260-4400, USA (Received-carbon steel in water, was found to improve the wettability of steel by water. The advancing contact angle

Chung, Deborah D.L.

431

Flood characteristics of the Buffalo River at Tyler Bend, Arkansas  

USGS Publications Warehouse

The Buffalo River is located in the Ozark Mountains in north-central Arkansas. Tyler Bend is on the Buffalo River about 1.5 miles upstream from U.S. Highway 65. The National Park Service is developing several recreational park sites along this scenic river. The magnitude, frequency, duration and velocities of floods are primary factors needed for establishing guidelines for developing facilities and managing park sites. The Park Service plans to develop park facilities at Tyler Bend and needs flood information at this site. This report provides information on the 100-, 75-, 50-, 30-, 20-, 10-, and 5-year floods on the Buffalo River at Tyler Bend. It was prepared by the U.S. Geological Survey in cooperation with the National Park Service and is based on data collected during the December 1982 flood, gaging station data for the Buffalo River near St. Joe, Arkansas and a Statewide flood-frequency report. (Lantz-PTT)

Neely, Braxtel L.

1987-01-01

432

INDUSTRIAL WASTE AND PRETREATMENT IN THE BUFFALO MUNICIPAL SYSTEM  

EPA Science Inventory

The requirements and affects of the combined treatment of industrial and domestic wastewaters were investigated for the Buffalo Sewer Authority's sewerage system. A comprehensive industrial waste survey was performed to obtain the required background information on industrial dis...

433

Seasonal dynamics of Schistosoma japonicum infection in buffaloes in the Poyang Lake region and suggestions on local treatment schemes.  

PubMed

Schistosomiasis japonica remains a major public health problem and the Poyang Lake region in Jiangxi province is one of the worst affected endemic areas. Buffaloes play a major role in the transmission of Schistosoma japonicum to humans. The aim of the present study was to increase understanding of the epidemic characteristics of schistosomiasis japonica in water buffaloes in the Poyang Lake region, after achieving the national mid-term goal, and to provide a basis for further interventions. The baseline prevalence in two villages in the Poyang Lake region in May 2010 was compared with respect to usage, sex and age in the total study population. Seasonal dynamics from May 2010 to May 2011 were observed in a natural village in the studied area. The baseline prevalence of infection in both villages (Caohui and Gaozhou) was 4.94% in May 2010. The prevalence in buffalo younger than 12 months was 12.82% in Caohui and 15.11% in Gaozhou, which was significantly higher than that found in those aged 13-24 months and older than 24 months. Of the 28 infected buffaloes, 82.14% (23) were younger than 12 months. The flow of seasonal dynamics showed that S. japonicum infection buffaloes were found from May to July and from November to January of the following year. This survey suggested that it is necessary to conduct two mass treatments (especially for young animals) in late March or early April and November, with an additional treatment of positive animals in July or June. PMID:24045056

Liu, Jin-Ming; Yu, Hua; Shi, Yao-Jun; Li, Hao; He, Liang; Li, Jian-Xi; Dong, Chang-Hua; Xie, Qiao; Jin, Ya-Mei; Lu, Ke; Lin, Jiao-Jiao

2013-11-15

434

Efficiency of ciprofloxacin for bacterial control, post-thaw quality, and in vivo fertility of buffalo spermatozoa.  

PubMed

Ciprofloxacin (CP) was evaluated for bacterial control, post-thaw quality, and fertility of buffalo semen. Pseudomonas aeruginosa, Escherichia coli, Proteus sp., Corynebacterium sp., Micrococcus sp., and Staphylococcus sp. were isolated from buffalo semen. Pseudomonas aeruginosa, Corynebacterium sp., and Micrococcus sp. were resistant to streptomycin, whereas P. aeruginosa and Proteus sp. were resistant to penicillin. All bacteria were susceptible to CP. In vitro dose toxicity was assessed in sodium citrate buffer containing 0, 200 to 2000 ?g/mL of CP. CP up to 1000 ?g/mL was found nontoxic to motility and viability of buffalo sperm. For post-thaw quality, buffalo semen was frozen in Tris-citric acid extender containing streptomycin-penicillin (SP; 1000 ?g/mL-1000 IU/mL) or CP 600 ?g/mL and was assessed for total aerobic bacterial count (post-thaw), motility, plasma membrane integrity, viability at 0, 2, and 4 hours post-thaw. At 4 hours post-thaw, plasma membrane integrity (%) was higher (P < 0.05) in extender containing CP than SP. Total aerobic bacterial count was 0.00 in extender containing CP compared with 0.07 × 10(4) cfu/mL with SP. To assess the in vivo fertility rate, semen (two bulls) frozen in Tris-citric acid extender containing SP or CP was used to inseminate, and 400 inseminations (200/group) were recorded. Higher (P ? 0.05) fertility rate was recorded with CP (55%) compared with SP (41%). In conclusion, use of CP in extender was efficient to control the bacterial contamination without compromising the post-thaw quality and fertility of cryopreserved water buffalo bull semen. PMID:23746693

Akhter, S; Ansari, M S; Rakha, B A; Andrabi, S M H; Qadeer, S; Iqbal, R; Ullah, N

2013-09-01

435

Repeated ovum pick-up in Italian Mediterranean buffalo cows  

Microsoft Academic Search

The potential of the ovum pick-up technique, used over a long period, was evaluated in 6 Italian Mediterranean buffalo cows that had more than 500 d open. The cows were submitted to ovum pick-up twice weekly for 2 mo. An additional 2-mo cycle of ovum pick-up was performed in 3 of the buffalo. The ovum pick-up sampling did not affect

R. Boni; S. Roviello; L. Zicarelli

1996-01-01

436

Liquefaction potential for New Uork state: a preliminary report on sites in Manhattan and Buffalo. Technical report  

SciTech Connect

The chances of a major earthquake occurring in New York in the near future is low. However, New York has experienced and no doubt will continue to experience moderate earthquakes. Catastrophic damage due to structural causes is not likely to occur for non-masonry buildings, but ground failure due to soil liquefaction is possible. It appears from the study that areas that lie adjacent to bodies of water (the Harlem River in Manhattan and Lake Erie in Buffalo) are liable to liquefy.

Budhu, M.; Vijayakumar, V.; Giese, R.F.; Baumgras, L.

1987-08-31

437

A COMPARATIVE STUDY OF THE WETTABILITY OF STEEL, CARBON, AND POLYETHYLENE FIBERS BY WATER  

E-print Network

A COMPARATIVE STUDY OF THE WETTABILITY OF STEEL, CARBON, AND POLYETHYLENE FIBERS BY WATER W. Lu, X at Buffalo, Buffalo, NY 14260-4400, USA (Received March 9, 1998; in final form April 10, 1998) ABSTRACT The wettability of fibers by water was found to increase in this order: polyethylene fiber, steel fiber

Chung, Deborah D.L.

438

Selectively maintained paleoviruses in Holarctic water fleas reveal an ancient origin for phleboviruses  

E-print Network

Selectively maintained paleoviruses in Holarctic water fleas reveal an ancient origin of Biological Sciences, The State University of New York at Buffalo, Buffalo, NY 14260, USA b A.N. Severtsov fresh- water habitats and has been the subject of multidisciplinary study for over half a century

Taylor, Derek

439

Geography & Map Resources: University of Buffalo Libraries  

NSDL National Science Digital Library

It's often difficult to find high-quality and well-curated lists of materials related to geography and cartography online. However, this collection created by David J. Bertuca, the subject librarian for geography at the University of Buffalo Libraries, serves as a rich trove of resources. The items here are divided into over a dozen headings, including Government Resources and Map Collections. Each area contains at least ten offerings with BertucaâÂÂs own salient commentary on the contents of each site, tool, collection, or application. The Selected Blogs feature is a real treat as it includes links to the fun and informative worlds of Google Maps Mania and Strange Maps. Visitors can also contact Bertuca directly to suggest links or to inquire about other resources.

440

BUFFALO PEAKS WILDERNESS STUDY AREA, COLORADO.  

USGS Publications Warehouse

Field investigations were conducted to evaluate the mineral-resource potential of the Buffalo Peaks Wilderness Study Area, Colorado. On the basis of this study there is a probable mineral-resource potential for silver vein and bedding replacement deposits along the Weston Pass fault zone, for hydrothermal vein-type uranium deposits in the vicinity of the Parkdale iron pit, and for gold vein deposits in the parts of the Granite and Four Mile districts that are within the wilderness study area. A probable barite resource potential occurs at Rough and Tumbling Creek and near Spring Creek on the east side of the study area. There is little promise for the occurrence of energy resources.

Hedlund, D.C.; Wood, R.H.

1984-01-01

441

Fish communities of the Buffalo River Basin and nearby basins of Arkansas and their relation to selected environmental factors, 2001-2002  

USGS Publications Warehouse

The Buffalo River lies in north-central Arkansas and is a tributary of the White River. Most of the length of the Buffalo River lies within the boundaries of Buffalo National River, a unit of the National Park Service; the upper 24 river kilometers lie within the boundary of the Ozark National Forest. Much of the upper and extreme lower parts of the basin on the south side of the Buffalo River is within the Ozark National Forest. During the summers of 2001 and 2002, fish communities were sampled at 52 sites in the study area that included the Buffalo River Basin and selected smaller nearby basins within the White River Basin in north-central Arkansas. Water quality (including nutrient and bacteria concentrations) and several other environmental factors (such as stream size, land use, substrate size, and riparian shading) also were measured. A total of 56 species of fish were collected from sites within the Buffalo River Basin in 2001 and 2002. All 56 species also were collected from within the boundaries of Buffalo National River. Twenty-two species were collected from headwater sites on tributaries of the Buffalo River; 27 species were collected from sites within or immediately adjacent to the Ozark National Forest. The list of species collected from Buffalo National River is similar to the list of species reported by previous investigators. Species richness at sites on the mainstem of the Buffalo River generally increased in a downstream direction. The number of s