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1

COMPARACIÓN DE VARIOS MÉTODOS PARA LA REPRESENTACIÓN CARTOGRÁFICA DE INFORMACIÓN CLIMÁTICA EN ZONAS ALTAS DEL ESTADO LARA  

Microsoft Academic Search

Se compararon tres métodos cartográficos diferentes para la representación de aspectos climáticos (temperatura y precipitación) en zonas altas del estado Lara. Se usó conjuntamente A) Representación analógica (en papel), B) Mapas digitalizados de coberturas mediante el software para Sistema de Información Geográfica (SIG) ArcInfo y C) Modelos numéricos interpolados mediante software de aplicación geoestadística (SURFER), empleando el modelo de interpolación

Jorge López Márquez; Rigoberto Andressen

2001-01-01

2

RELACIÓN SUELO-ÁRBOL Y FACTORES DE SITIO, EN PLANTACIONES JÓVENES DE TECA (Tectona grandis), EN LA ZONA OESTE DE LA CUENCA DEL CANAL DE PANAMÁ  

Microsoft Academic Search

Recibido: 07\\/12\\/04 Aceptado: 01\\/03\\/05 RE SU MEN Mediante la utilización de un muestreo factorial estratificado con 4 repeticiones (3x3x4) se seleccionaron 36 parcelas permanentes de medición en plantaciones de teca en la zona oeste de la cuenca del Canal de Panamá. En la estratifi- cación se emplearon como criterios el incremento medio anual (IMA) en altura total y la pendiente

Manuel Mollinedo; Luis Ugalde; Alfredo Alvarado; Jean Mark Verjans; Luis Carles Rudy

2005-01-01

3

Evaluación paramétrica y no paramétrica del desempleo pesquero en Marruecos: Una aplicación para el caso andaluz  

Microsoft Academic Search

RESUMEN El principal objetivo es el análisis de las medidas que las diferentes administraciones pusieron en marcha para reducir las altas tasas de desempleo en la población andaluza afectada por la paralización de la actividad pesquera como consecuencia de la finalización del Acuerdo de Pesca entre la Unión Europea y Marruecos desde el 30 de noviembre de 1999. Mediante la

Encarnación Cordón Lagares; Félix García Ordaz

2010-01-01

4

Zona Land: Wave Adder  

NSDL National Science Digital Library

This item is an interactive Java simulation that models the addition and subtraction of waves. You can set amplitude, frequency, and phase shift of two waves, then push the "Add" button to see the wave addition on a coordinate plane. The student may select either addition or subtraction and may opt for either sine or cosine functions. These buttons are at the lower left (their text is hard to see). SEE RELATED ITEMS ON THIS PAGE for a link to the full set of Zona Land Waves tutorials.

Zobel, Edward

2009-03-22

5

Las perspectivas de las zonas económicas especiales de exportación  

Microsoft Academic Search

Con base en la actual globalización mundial de la economía y de la interdependencia comercial, tecnológica y financiera de los países y de la experiencia de la apertura económica de China, el gobierno colombiano propone la creación de las Zonas Económicas Especiales de Exportación, ZEEE, en los departamentos de Nariño, Valle del Cauca, Norte de Santander y Cesar para aumentar

Alexander Arévalo Luna; Paula Lucia Arevalo M

2002-01-01

6

Zonadhesin: characterization, localization, and zona pellucida binding.  

PubMed

Zonadhesin is a multiple-domain transmembrane protein that is believed to function as a sperm-zona pellucida binding protein. In this study we sequenced zonadhesin from rabbit testis and analyzed its processing, expression, localization, and zona pellucida binding. We show that the precursor protein occurs exclusively in the testis and that proteolytic processing results in the formation of three fragments: p43 (D1 domain), p97 (D2-D4 domains), and p58 (D4 domain-C-terminal). In mature spermatozoa the p43 and p97 fragments exist as disulfide-bonded dimers. During spermatogenesis, synthesis of zonadhesin mRNA chiefly occurs in primary spermatocytes, whereas the protein is abundant in both Sertoli cells and spermatids. In spermatozoa the protein is localized exclusively to the anterior acrosome but is not available for binding antibody on live spermatozoa. Once the acrosome reaction is induced, zonadhesin is lost from the spermatozoon, but remains with the acrosomal shroud. We show that recombinant D4 domain can bind zona pellucida, and we propose that zonadhesin functions after the acrosome reaction has been initiated to bind the acrosomal shroud to the zona pellucida. PMID:11717130

Lea, I A; Sivashanmugam, P; O'Rand, M G

2001-12-01

7

Exocytosis in Spermatozoa in Response to Progesterone and Zona Pellucida  

Microsoft Academic Search

Exocytosis in mammalian spermatozoa (the acrosome reaction) is a process essential for fertilization. Both progesterone and zona pellucida induce exocytosis in spermatozoa, which may encounter both during penetration of the oocyte's vestments. When mouse spermatozoa were exposed first to progesterone and then to zona pellucida, exocytosis was enhanced to a greater degree than that seen when the agonists were presented

Eduardo R. S. Roldan; Tetsuma Murase; Qi-Xian Shi

1994-01-01

8

Acrosome reaction: relevance of zona pellucida glycoproteins  

PubMed Central

During mammalian fertilisation, the zona pellucida (ZP) matrix surrounding the oocyte is responsible for the binding of the spermatozoa to the oocyte and induction of the acrosome reaction (AR) in the ZP-bound spermatozoon. The AR is crucial for the penetration of the ZP matrix by spermatozoa. The ZP matrix in mice is composed of three glycoproteins designated ZP1, ZP2 and ZP3, whereas in humans, it is composed of four (ZP1, ZP2, ZP3 and ZP4). ZP3 acts as the putative primary sperm receptor and is responsible for AR induction in mice, whereas in humans (in addition to ZP3), ZP1 and ZP4 also induce the AR. The ability of ZP3 to induce the AR resides in its C-terminal fragment. O-linked glycans are critical for the murine ZP3-mediated AR. However, N-linked glycans of human ZP1, ZP3 and ZP4 have important roles in the induction of the AR. Studies with pharmacological inhibitors showed that the ZP3-induced AR involves the activation of the Gi-coupled receptor pathway, whereas ZP1- and ZP4-mediated ARs are independent of this pathway. The ZP3-induced AR involves the activation of T-type voltage-operated calcium channels (VOCCs), whereas ZP1- and ZP4-induced ARs involve both T- and L-type VOCCs. To conclude, in mice, ZP3 is primarily responsible for the binding of capacitated spermatozoa to the ZP matrix and induction of the AR, whereas in humans (in addition to ZP3), ZP1 and ZP4 also participate in these stages of fertilisation.

Gupta, Satish K; Bhandari, Beena

2011-01-01

9

Transcriptome Analysis Reveals Differentially Expressed Transcripts in Rat Adrenal Zona Glomerulosa and Zona Fasciculata  

PubMed Central

In mammals, aldosterone is produced in the zona glomerulosa (zG), the outermost layer of the adrenal cortex, whereas glucocorticoids are produced in adjacent zona fasciculata (zF). However, the cellular mechanisms controlling the zonal development and the differential hormone production (i.e. functional zonation) are poorly understood. To explore the mechanisms, we defined zone-specific transcripts in this study. Eleven-week-old male rats were used and adrenal tissues were collected from zG and zF using laser-capture microdissection. RNA was isolated, biotin labeled, amplified, and hybridized to Illumina microarray chips. The microarray data were compared by fold change calculations. In zG, 235 transcripts showed more than a 2-fold up-regulation compared to zF with statistical significance. Similarly, 231 transcripts showed up-regulation in zF. The microarray findings were validated using quantitative RT-PCR and immunohistochemical staining on selected transcripts, including Cyp11b2 (zG/zF: 214.2x), Rgs4 (68.4x), Smoc2 (49.3x), and Mia1 (43.1x) in zG as well as Ddah1 (zF/zG 16.2x), Cidea (15.5x), Frzb (9.5x), and Hsd11b2 (8.3x) in zF. The lists of transcripts obtained in the current study will be an invaluable tool for the elucidation of cellular mechanisms leading to zG and zF functional zonation.

Nishimoto, Koshiro; Rigsby, Christine S.; Wang, Tao; Mukai, Kuniaki; Gomez-Sanchez, Celso E.; Rainey, William E.

2012-01-01

10

El valor de uso recreativo de los espacios naturales protegidos. Una aplicación de los métodos de valoración contingente y del coste del viaje  

Microsoft Academic Search

En este articulo se presenta una aplicación de dos de las metodologías desarrolladas para medir los beneficios que se derivan del uso recreativo de los bienes ambientales en ausencia de mercado, el método del coste del viaje (MCV) y el de valoración contingente (MVC). La zona objeto de estudio ha sido el Parque Nacional de “Aigüestortes y Estany de Sant

M. FARRÉ PERDIGUER

2003-01-01

11

In vitro modification of rat adrenal zona fasciculata/reticularis function by the zona glomerulosa.  

PubMed

Following earlier findings, the possibility that the zona glomerulosa of the rat adrenal cortex may modify inner zone function was investigated. In a series of in vitro experiments designed to test this hypothesis it was found that: 1. Inner zone preparations gave significantly more steroid than either glomerulosa tissue incubated alone, or (for the first 40 min of a two hour incubation), an equivalent amount of whole adrenal tissue. Whole tissue yields were greater than glomerulosa alone only at the 120 min point. At no time did the whole tissue output approach the total anticipated by addition of separate inner zone and glomerulosa yields. 2. In 20 min incubations, inner zone steriod output generally could be depressed by the addition of a glomerulosa preincubation medium (PIM), or a steroid extract of a glomerulosa PIM, or by the addition of 100 ng aldosterone. Inner zone PIM had less effect. 3. Higher concentrations of aldosterone gave increased inhibition of inner zone corticosteroid production. The results indicated that only a part of the total corticosterone output was aldosterone sensitive in this way. 4. More specific analysis of the steroids showed that yields of corticosterone from endogenous precursors were depressed by aldosterone, but deoxycorticosterone and 18-hydroxydeoxycorticosterone were not. In the same incubations the presence of aldosterone had no effect on the formation of these three products from [3H]pregnenolone. In total the results suggest that the presence of the glomerulosa may affect inner zone function by inhibiting the secretion of corticosterone formed from endogenous precursors. It is possible that aldosterone is the agent of this effect. PMID:946150

Vinson, G P; Whitehouse, B J

1976-02-01

12

Outcomes of preimplantation genetic diagnosis using either zona drilling with acidified Tyrode's solution or partial zona dissection  

PubMed Central

Objective To review the outcomes of preimplantation genetic diagnosis (PGD) using zona drilling with acid Tyrode's solution (chemical zona pellucida drilling, chemical ZD) and those of partial zona dissection (PZD). Methods Clinical outcomes of seventy-one couples undergoing 85 PGD cycles from January 2005 to December 2010 were included. Blastocyst formation and the hatching rate, clinical pregnancy rate, ongoing pregnancy rate, implantation rate, and fetal gender ratio of the PZD and chemical ZD groups were compared. Results Application of PZD resulted in a significantly higher rate of clinical pregnancy (40.7% vs. 15.4%, p=0.022), ongoing pregnancy (35.6% vs. 11.5%, p=0.023), and implantation (18.1% vs. 5.7%, p=0.007) compared with chemical ZD. Among non-transferred embryos, the rate of blastocyst formation on day 5 (49.1% vs. 39.5%, p=0.016) and hatching on day 6 (47.2% vs. 26.5%, p<0.001) were also significantly higher in the PZD group. Conclusion The mechanical zona dissection method showed better outcomes than chemical ZD in terms of the blastocyst development and pregnancy rate. In this study, the fact that chemical ZD was conducted in different period from mechanical method should be considered in interpreting the result.

Kim, Hyun Jung; Kim, Chung Hyon; Lee, Soo Min; Choe, Seung Ah; Lee, Joong Yeup; Jee, Byung Chul; Hwang, Doyeong

2012-01-01

13

Effect of nitric oxide on rat adrenal zona fasciculata steroidogenesis  

Microsoft Academic Search

The present study was designed to investigate the role of nitric oxide (NO) in the regulation of adreno- cortical function. DiVerent NO donors, such as sodium nitroprusside (SNP), S-nitroso-l-acetyl penicillamine, diethylamine\\/NO complex sodium salt and diethylene- triamine NO adduct, significantly decreased cortico- sterone production both in unstimulated and in corticotropin-stimulated zona fasciculata adrenal cells, in a dose-dependent manner. The eVect

C B Cymeryng; L A Dad; E J Podesta

1998-01-01

14

Zona-free and with-zona parthenogenetic embryo production in goat ( Capra hircus) — effect of activation methods, culture systems and culture media  

Microsoft Academic Search

Studies on parthenogenetic activation of oocytes are important to improve the efficiency of nuclear transfer as artificial activation of oocytes is an essential component of nuclear transfer protocol. The present study was carried out to investigate the effects of different activation methods, culture systems and culture media on in vitro development of zona-free and with-zona parthenogenetic embryos in goat. In

Arun Kumar De; Dhruba Malakar; Manoj Kumar Jena; Rahul Dutta; Shweta Garg; Yogesh S. Akshey

15

Interaction of acrosome-reacted macaque sperm with the macaque zona pellucida.  

PubMed

In this study we investigated the ultrastructure of macaque sperm induced to acrosome-react with calcium ionophore A23187, and the interaction between these acrosome-reacted sperm and the macaque zona pellucida. Transmission electron microscopy revealed that the majority of ionophore-treated sperm retained the vesiculated acrosomal cap or "shroud." Untreated, acrosome-reacted sperm on the zona had a similar ultrastructural appearance. In sperm-zona binding experiments, a mean of 4.5 ionophore-treated sperm were bound per zona after 1 min of coincubation compared with 41 sperm per zona in the solvent control. Vigorous pipetting was used to remove the acrosomal shrouds from approximately 50% of acrosome-reacted sperm before incubation with oocytes. Significantly more of these mechanically treated sperm were bound to the zona after a 4-min coincubation compared with acrosome-reacted sperm that were not pipetted. The number of mechanically treated sperm bound to the zona was the same whether the sperm and oocytes were coincubated in calcium-free medium or in control medium. The percentage of mechanically treated sperm that were acrosome-reacted on the zona also was not different in the two media. We conclude that macaque sperm that undergo the acrosome reaction on the zona surface are bound by the acrosomal shroud before zona penetration. When sperm acrosome-react before interaction with the oocyte, their zona binding capacity is significantly reduced. Removal of the acrosomal shroud and exposure of the inner acrosomal membrane increases the affinity of sperm for the zona. This sequence occurs naturally during the transition from primary binding to secondary binding on the zona surface. PMID:9160732

VandeVoort, C A; Yudin, A I; Overstreet, J W

1997-05-01

16

PH-20 but not acrosin is involved in sperm penetration of the macaque zona pellucida.  

PubMed

In this study, we investigated the functions of PH-20 and acrosin during the interaction of macaque sperm with the zona pellucida. Both of these sperm enzymes have been reported to be present on the inner acrosomal membrane of acrosome reacted sperm, and have been suggested to play a role during secondary sperm-zona binding in other species. Anti-macaque PH-20 IgG, anti-pig acrosin IgG and soybean trypsin inhibitor (SBTI) were used as probes for immunolocalization of the two proteins at the ultrastructural level, and as reagents for blocking sperm penetration of the macaque zona pellucida in vitro. As a control, we performed similar studies with antibodies to CD-46, which is also located on the inner acrosomal membrane, but has no known function in sperm-zona pellucida interaction. After labeling with anti-acrosin IgG, gold label was not present on the sperm surface before the acrosome reaction, but was detected over the entire head of sperm that were induced to acrosome react with calcium ionophore A23187. In contrast, when sperm were induced to acrosome react by binding to intact zona pellucida, acrosin was present in the acrosomal shroud but not on the inner acrosomal membrane. Similar results were obtained when SBTI was used as a probe for enzyme localization. PH-20 and CD-46 were demonstrated on the inner acrosomal membrane of sperm induced to acrosome react by ionophore treatment and by zona binding. Neither anti-acrosin IgG nor anti-CD-46 IgG affected sperm penetration of the zona at concentrations up to 300 microg/ml, but zona penetration was blocked completely when anti-PH-20 IgG (100 microg/ml) was present during sperm-oocyte interaction. Ultrastructural observations of oocytes incubated with anti-PH-20 IgG showed that acrosomal shrouds were present on the zona surface but no sperm had begun to penetrate into the zona substance. We conclude that anti-PH-20 IgG prevented sperm penetration of the macaque zona pellucida by interference with secondary sperm-zona binding, rather than primary sperm-zona binding or the zona-induced acrosome reaction. Acrosin was not detected on the inner acrosomal membrane of sperm that are induced to acrosome react after zona binding, and acrosin does not appear to be critical for sperm penetration of the macaque zona pellucida. PMID:10369396

Yudin, A I; Vandevoort, C A; Li, M W; Overstreet, J W

1999-07-01

17

Componentes naturales y de uso del suelo vulnerables a las variaciones del nivel del mar en la costa atlántica de México  

Microsoft Academic Search

Palabras clave Regionalizaci6n de costas, fislografia, vulnerabilidad costera, uso del suelo Absúact Geomorphological alaradenstia of the mastal iongitudinal prn* and the arrangwent of the physiographical stmcture allowed the rmnition of two zonas: a) the intertidai zone -a basic sntne for dired imoaci of sea leve1 dame: and b) an adjacent peritidál (supralittoral) hnge &n area of cushioning or of polential

Mano Arturo; Ortiz Pérez; Ana Patricia; Méndez Linares

18

Inhibitory role of the zona incerta in the pilocarpine model of epilepsy  

Microsoft Academic Search

Recent experiments have suggested that the zona incerta might be regarded as a highly sensitive structure for seizure induction. This sensitivity has been linked to this structure's abundant expression of cholinergic receptors. Here we have decided to investigate the participation of the GABAergic system of the zona incerta, one of its major neurotransmitters with widespread projections to the neocortex, in

Clement Hamani; Sumire Sakabe; Zuner A. Bortolotto; Esper A. Cavalheiro; Luiz E. A. M. Mello

2002-01-01

19

L’eruzione laterale etnea del novembre 1950 — dicembre 1951  

Microsoft Academic Search

Conclusione  L’eruzione etnea del 1950–51 si manifestata quasi senza segni premonitori, dopo una breve (meno di un anno) stasi del vulcano.\\u000a Essa si stabilita in una zona che stata gi teatro di precedenti eruzioni (1811) ed ha avuto un’attivit prevalentemente\\u000a effusiva di notevole durata. La lava emessa ha la composizione mineralogica identica alle lave emesse in questo secolo; essa\\u000a si differenzia

Gustavo Cumin

1954-01-01

20

Etnografía de la infección respiratoria aguda en una zona rural del altiplano mexicano  

Microsoft Academic Search

Objective. To identify the terms used by mothers to refer to diseases, signs and symptoms related to acute respi- ratory illnesses (ARI), alarming signs which should moti- vate them to seek medical attention, and to describe common home practices of disease care and treatment. Material and methods. An ethnographic study was per- formed in six rural communities of the Mexican

HOMERO MARTÍNEZ; KIMBERLY SURIANO; GERY W. RYAN; GRETEL H. PELTO

1997-01-01

21

Formation of zona radiata and ultrastructural analysis of egg envelope during oogenesis of Chinese perch Siniperca chuatsi  

Microsoft Academic Search

During oocyte maturation, egg envelope undergoes a series of changes in structure and function. We investigated the follicular envelope, zona radiata and plasmalemma of Chinese perch, Siniperca chuatsi at five different developmental stages by light, scanning and transmission electron microscopy. An octopus-like structure in the zona radiata was observed for the first time. The increasing thickness of zona radiata during

Ye-Qin Jiang; Tian-Ting Zhang; Wan-Xi Yang

2010-01-01

22

ANGIOTENSIN II REGULATES ADRENAL VASCULAR TONE THROUGH ZONA GLOMERULOSA CELL-DERIVED EETS AND DHETS  

PubMed Central

Elevated concentrations of aldosterone are associated with several cardiovascular diseases. Angiotensin II increases aldosterone secretion and adrenal blood flow. This concurrent increase in steroidogenesis and adrenal blood flow is not understood. We investigated the role of zona glomerulosa cells in the regulation of vascular tone of bovine adrenal cortical arteries by angiotensin II. Zona glomerulosa cells enhance endothelium-dependent relaxations to angiotensin II. The zona glomerulosa cell-dependent relaxations to angiotensin II are unchanged by removing the endothelium-dependent response to angiotensin II. These zona glomerulosa cell-mediated relaxations are ablated by cytochrome P450 inhibition, epoxyeicostrienoic acid antagonism, and potassium channel blockade. Analysis of zona glomerulosa cell epoxyeicosatrienoic acid production by liquid chromatography/mass spectrometry demonstrates an increase in epoxyeicosatrienoic and dihydroxyeicosatrienoic acids with angiotensin II stimulation. These epoxyeicosatrienoic and dihydroxyeicosatrienoic acids produced similar concentration-dependent relaxations of adrenal arteries, which were attenuated by epoxyeicosatrienoic acid antagonism. Whole cell potassium current of adrenal artery smooth muscle cells were increased by angiotensin II stimulation in the presence of zona glomerulosa cells but decreased in the absence of zona glomerulosa cells. This increase in potassium current was abolished by iberiotoxin. Similarly, 14,15-epoxyeicosatrienoic acid induced concentration-dependent increases in potassium current, which was abolished by iberiotoxin. Zona glomerulosa cell aldosterone release is not directly altered by epoxyeicosatrienoic acids. These data suggest that angiotensin II stimulates zona glomerulosa cells to release epoxyeicosatrienoic and dihydroxyeicosatrienoic acids, resulting in potassium channel activation and relaxation of adrenal arteries. This provides a mechanism by which Ang II concurrently increases adrenal blood flow and steroidogenesis.

Kopf, Phillip G.; Gauthier, Kathryn M.; Zhang, David X.; Falck, John R.; Campbell, William B.

2011-01-01

23

Welcome to Zona Land: Education in Physics and Mathematic  

NSDL National Science Digital Library

Zona Land, created by Ed Zobel, is a science and mathematics resource site for students and teachers. The site illustrates mathematical and physical principles by the use of Java applets and VRML simulations. The Science section contains the Physics Department (mechanics, waves and light), EZ Graph, and EZ Evaluator. EZ Graph allows the user to view the graph of a desired function. Evaluator is an online calculator that has trigonometric capabilities. The Mathematics section has four subject areas plus the Graph and Evaluator tools. Graph Paper allows the user to examine the effects of different scales on pre-designed linear, cubic, quadratic, and sine functions. The Function Institute begins with an optional tour of the definition of a function. Also available at the Institute are graphs of linear and polynomial functions. The Geometry Section has three areas of resources: Common Shapes, Fractals, and Coordinate Geometry. Trigonometry Realms has a simple yet comprehensive introduction to trigonometry. Help is available for all of the different resource applications.

Zobel, Edward A.

1998-01-01

24

Scanning electron microscopy of the zona pellucida of human oocytes during intracytoplasmic sperm injection (ICSI).  

PubMed

During intracytoplasmic sperm injection (ICSI) approximately 10% of all injected oocytes degenerate. The reason for this process is unknown. It has been speculated that the mechanical procedure of the insertion of the ICSI needle induces injuries to the zona pellucida which lead to the death of the cell. By scanning electron microscope (SEM), it could be shown that the surface structure of mature oocytes is extremely elastic so that the injection needle penetrates the zona pellucida without destroying the mesh-like or more compact surface. No tissue pieces or zona fragments were detectable. After a culture time of 15 min the penetration site on the zona was no longer easily visible. We believe that oocyte degeneration is not caused by the penetration of a glass needle into the ooplasm but by an injury to the meiotic spindle or by an excessive dose of fluid [polyvinylpyrrolidone (PVP) or medium] during sperm injection. PMID:9021374

Schwartz, P; Magerkurth, C; Michelmann, H W

1996-12-01

25

Loss of Zona Pellucida Binding Proteins in the Acrosomal Matrix Disrupts Acrosome Biogenesis and Sperm Morphogenesis  

Microsoft Academic Search

Zona pellucida binding protein 1 (ZPBP1), a spermatid and spermatozoon protein that localizes to the acrosome, was originally identified in pigs and named for its binding to the oocyte zona pellucida. In an in silico search for germ cell-specific genes, Zpbp1 and its novel paralog, Zpbp2, were discovered and confirmed to be expressed only in the testes in both mice

Yi-Nan Lin; Angshumoy Roy; Wei Yan; Kathleen H. Burns; Martin M. Matzuk

2007-01-01

26

Immunocontraception of white-tailed deer using native and recombinant zona pellucida vaccines  

Microsoft Academic Search

We conducted a 2-year feasibility study with native porcine zona pellucida (PZP) vaccine and three recombinant rabbit zona pellucida vaccines (RC55, RC75a and a combination of RC55, RC75a and RC75b) as an initial phase of developing a recombinant immunocontraceptive vaccine to control reproduction in overpopulated herds of white-tailed deer (Odocoileus virginianus). Forty captive white-tailed does were divided into five groups

Lowell A. Miller; Brad E. Johns; Gary J. Killian

2000-01-01

27

Disabled2 Is Expressed in Adrenal Zona Glomerulosa and Is Involved in Aldosterone Secretion  

Microsoft Academic Search

The differentiation of the adrenal cortex into functionally specific zones is probably due to differential temporal gene expression during fetal growth, development, and adult- hood. In our search for adrenal zona glomerulosa-specific genes, we found that Disabled-2 (Dab2) is expressed in the zona glomerulosa of the rat adrenal gland using a combi- nation of laser capture microdissection, mRNA amplifica- tion,

Damian G. Romero; Licy L. Yanes; Angela F. de Rodriguez; Maria W. Plonczynski; Bronwyn L. Welsh; Jane F. Reckelhoff; Elise P. Gomez-Sanchez; Celso E. Gomez-Sanchez

2007-01-01

28

Three-dimensional partial zona dissection for preimplantation genetic diagnosis and assisted hatching  

Microsoft Academic Search

Objective: To develop a new approach to partial zona dissection of oocytes and embryos for facilitating preimplantation genetic sampling and assisted hatching.Design: Controlled clinical study.Setting: Preimplantation genetic diagnosis and IVF program, Reproductive Genetics Institute\\/IVF Illinois, Chicago, Illinois.Patient(s): Three hundred forty patients undergoing IVF in whom preimplantation genetic diagnosis or assisted hatching was required.Intervention(s): Three-dimensional partial zona dissection and conventional partial

Jeanine Cieslak; Victor Ivakhnenko; Georg Wolf; Svetlana Sheleg; Yury Verlinsky

1999-01-01

29

The influence of anti-zona and anti-sperm antibodies on sperm--egg interactions.  

PubMed

Anti-zona antibodies are effective inhibitors of fertilization in vitro and, regardless of whether passive or active immunization techniques are used, in vivo. Antibodies raised against unfractionated zona pellucida antigens are chiefly directed against a group of carbohydrate-rich components localized on the outer surface of the zona. The interaction of anti-zona antibodies with these sites induces the formation of a surface precipitate which occludes the sperm binding sites by a process of steric hindrance, and stabilizes the zona structure against digestion by the proteolytic enzymes of the sperm head. Active immunization studies indicate that the long-term induction of infertility without adverse side effects is feasible in both laboratory rodents and primates when the zona pellucida is used as a target. Anti-sperm antibodies also exhibit a capacity for inhibiting fertilization in vivo and in vitro. To determine the most appropriate detection method to screen patients for anti-sperm antibodies several homologous and heterologous antisera were analysed by 5 different agglutination and immobilization techniques and then compared for their ability to inhibit the fertilizing capacity of human spermatozoa using the zona-free hamster egg penetration test. The results obtained with the Franklin--Dukes tube--slide test exhibited the closest correlation with the anti-fertility activity of a given antiserum; this activity could be amplified by the addition of complement to the medium. It is concluded that antibodies directed against the sperm head are responsible for limiting the fertilizing capacity of human spermatozoa in vitro and that it is these antibodies of which attention should be focused to unravel the role that immunological factors play in the aetiology of infertility in vivo. PMID:7019431

Aitken, R J; Rudak, E A; Richardson, D W; Dor, J; Djahanbahkch, O; Templeton, A A

1981-07-01

30

Ultrastructural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs  

Microsoft Academic Search

Receptors for Ricinus communis agglutinin I (RCAt), concanavalin A (Con A), and wheat germ agglutinin (WGA) were localized on the zonae pellucidae and plasma membranes of hamster, mouse, and rat eggs with ferritin-lectin conjugates. Intact eggs labeled with the ferritin conjugates showed dense concentrations of RCA~ and WGA receptors in the outermost regions of their zonae pellucidae and sparse distributions

GARTH L. NICOLSON; RYUZO YANAGIMACHI; HIROKO YANAGIMACHI

1975-01-01

31

Experimental Study of the Developing Mammalian Egg: Removal of the Zona Pellucida  

Microsoft Academic Search

The zona pellucida may be removed from all stages of the mouse egg by digestion with pronase. Cumulus and corona cells are also dispersed by the enzyme. No change in membrane disgestibility occurs at fertilization. In tests thus far of two-cell eggs and later stages, development continues. Blastocysts exhibit \\

Beatrice Mintz

1962-01-01

32

CLINICAL ASSISTED REPRODUCTION: Births After Transfer of Zona-Free Blastocysts in Oocyte Donation Cycles  

Microsoft Academic Search

Purpose: Oocyte donation is a well-established method of assisted reproduction for women with irreversible infertility and with previous implantation failures after in vitro fertilization. Although the pregnancy rates are very high, sometimes implantation does not occur even after various attempts. We report the first two cases of transfer of zona-free blastocysts in oocyte donation cycles that developed to normal pregnancies

Marcos A. C. Sampaío; Selmo Geberw

2001-01-01

33

Human ZP4 is not sufficient for taxon-specific sperm recognition of the zona pellucida in transgenic mice  

PubMed Central

The molecular basis of human fertilization remains enigmatic. Mouse models are often used to study sperm–egg recognition, but the mouse zona pellucida surrounding ovulated eggs contains three proteins (ZP1, ZP2, and ZP3) whereas the human zona contains four (ZP1, ZP2, ZP3, and ZP4). Human sperm are fastidious and recognize human but not mouse eggs. Transgenic mouse lines were established to ascertain whether human ZP4 is the sole determinant of human sperm binding. Human ZP4 expressed in transgenic mice had a molecular mass similar to the range of native protein isoforms and was incorporated into the extracellular zona matrix. Transgenic females were fertile with normal litter sizes. Mouse sperm readily recognized transgenic ovulated eggs, but human sperm did not. We conclude that human ZP4 is not sufficient to support human sperm binding to the zona pellucida in transgenic mice and that other zona proteins may be needed for human gamete recognition.

Yauger, Belinda; Boggs, Nathan A; Dean, Jurrien

2012-01-01

34

Adrenic acid metabolites as endogenous endothelium-derived and zona glomerulosa-derived hyperpolarizing factors.  

PubMed

Adrenic acid (docosatetraenoic acid), an abundant fatty acid in the adrenal gland, is identical to arachidonic acid except for 2 additional carbons on the carboxyl end. Adrenic acid is metabolized by cyclooxygenases, cytochrome P450s, and lipoxygenases; however, little is known regarding the role of adrenic acid and its metabolites in vascular tone. Because of its abundance in the adrenal gland, we investigated the role of adrenic acid in vascular tone of bovine adrenal cortical arteries and its metabolism by bovine adrenal zona glomerulosa cells. In adrenal cortical arteries, adrenic acid caused concentration-dependent relaxations, which were inhibited by the epoxyeicosatrienoic acid antagonist 14,15-epoxyeicosa-5(Z)-enoic acid and the cytochrome P450 inhibitor SKF-525A. The large-conductance calcium-activated potassium channel blocker iberiotoxin or removal of the endothelium abolished these relaxations. Reverse-phase high-pressure liquid chromatography and liquid chromatography/mass spectrometry isolated and identified numerous adrenic acid metabolites from zona glomerulosa cells, including dihomo-epoxyeicosatrienoic acids and dihomo-prostaglandins. In denuded adrenal cortical arteries, adrenic acid caused concentration-dependent relaxations in the presence of zona glomerulosa cells but not in their absence. These relaxations were inhibited by SKF-525A, 14,15-epoxyeicosa-5(Z)-enoic acid, and iberiotoxin. Dihomo-16,17-epoxyeicosatrienoic acid caused concentration-dependent relaxations of adrenal cortical arteries, which were inhibited by 14,15-epoxyeicosa-5(Z)-enoic acid and high potassium. Our results suggest that adrenic acid relaxations of bovine adrenal cortical arteries are mediated by endothelial and zona glomerulosa cell cytochrome P450 metabolites. Thus, adrenic acid metabolites could function as endogenous endothelium-derived and zona glomerulosa-derived hyperpolarizing factors in the adrenal cortex and contribute to the regulation of adrenal blood flow. PMID:20038752

Kopf, Phillip G; Zhang, David X; Gauthier, Kathryn M; Nithipatikom, Kasem; Yi, Xiu-Yu; Falck, John R; Campbell, William B

2009-12-28

35

Fotometría CCD en el campo del cúmulo abierto Ruprecht 58  

NASA Astrophysics Data System (ADS)

La región de Vela-Puppis contiene varios cúmulos cuyo estudio puede brindarnos información acerca de la cinemática y el estado evolutivo de la población estelar en las zonas externas del disco galáctico. A través de observaciones CCD en los filtros UBV en el campo del cúmulo abierto Ruprecht 58 (?2000= 8h 14m 41s, ?2000=-31o 57'), se han determinado por primera vez los parámetros fundamentales de este cúmulo tales como distancia, enrojecimiento y edad. Se analiza también la estructura de su secuencia principal y la presencia de gigantes y binarias.

Giorgi, E. E.; Vázquez, R. A.; Seggewiss, W.; Ostrov, P.; Solivella, G. R.

36

Atlas del Genoma del Cáncer  

Cancer.gov

El Atlas del Genoma del Cáncer es una iniciativa de los Institutos Nacionales de la Salud (NIH) para crear mapas multidimensionales completos de los cambios genómicos clave en los tipos y subtipos principales de cáncer.

37

Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization  

PubMed Central

To fuse with oocytes, spermatozoa of eutherian mammals must pass through extracellular coats, the cumulus cell layer, and the zona pellucida (ZP). It is generally believed that the acrosome reaction (AR) of spermatozoa, essential for zona penetration and fusion with oocytes, is triggered by sperm contact with the zona pellucida. Therefore, in most previous studies of sperm–oocyte interactions in the mouse, the cumulus has been removed before insemination to facilitate the examination of sperm–zona interactions. We used transgenic mouse spermatozoa, which enabled us to detect the onset of the acrosome reaction using fluorescence microscopy. We found that the spermatozoa that began the acrosome reaction before reaching the zona were able to penetrate the zona and fused with the oocyte's plasma membrane. In fact, most fertilizing spermatozoa underwent the acrosome reaction before reaching the zona pellucida of cumulus-enclosed oocytes, at least under the experimental conditions we used. The incidence of in vitro fertilization of cumulus-free oocytes was increased by coincubating oocytes with cumulus cells, suggesting an important role for cumulus cells and their matrix in natural fertilization.

Jin, Mayuko; Fujiwara, Eiji; Kakiuchi, Yasutaka; Okabe, Masaru; Satouh, Yuhkoh; Baba, Shoji A.; Chiba, Kazuyoshi; Hirohashi, Noritaka

2011-01-01

38

Lessons from the gene expression pattern of the rat zona glomerulosa.  

PubMed

We recently identified hundreds of transcripts with differential expression in rat zona glomerulosa (zG) and zona fasciculata. Although the genes up-regulated in the zG may be playing important roles in aldosterone production, the relationship between most of these genes and aldosterone production has not been uncovered. Because aldosterone, in the presence of a high sodium diet, is now considered a significant cardiovascular risk factor, in this review we performed gene ontology and pathway analyses on the same microarray data to better define the genes that may influence zG function. Overall, we identified a number of genes that may be involved in aldosterone production through transforming growth factor ? (TGF-?), WNT, calcium, potassium, and ACTH signaling pathways. The list of genes we present in the current report may become an important tool for researchers working on primary aldosteronism and aldosterone-related cardiovascular diseases. PMID:23287491

Nishimoto, Koshiro; Rainey, William E; Bollag, Wendy B; Seki, Tsugio

2012-12-31

39

Primate recombinant zona pellucida proteins expressed in Escherichia coli bind to spermatozoa  

Microsoft Academic Search

To delineate the role of individual zona pellucida (ZP) glycoproteins during sperm–oocyte interaction, bonnet monkey (bm; Macaca radiata) ZPA (bmZPA), ZPB (bmZPB), and ZPC (bmZPC) have been cloned without native signal sequence and transmembrane-like domain, and expressed in Escherichia coli. Recombinant proteins have been purified from the inclusion bodies in presence of low concentration of chaotropic agent (2 M urea)

Gagandeep K Gahlay; Neelu Srivastava; Chhabi K Govind; Satish K Gupta

2002-01-01

40

Laser-assisted hatching of cryopreserved—thawed embryos by thinning one quarter of the zona  

Microsoft Academic Search

Laser-assisted hatching is little documented in the literature regarding its efficacy in cryopreserved—thawed (CT) embryo transfer cycles. The aim of the present study was to evaluate in a randomized manner the efficacy of thinning one quarter of the zona pellucida of CT embryos to a depth of 50–80% of the original thickness, via laser treatment (the qLZT-AH procedure), in improving

Claudia Guilhermino Petersen; Ana Lucia Mauri; Ricardo Luiz Razera Baruffi; João Batista Alcãntara Oliveira; Valeria Felipe; Fabiana Cagnoto Massaro; Jose Gonçalves Franco Jr

2006-01-01

41

Zona pellucida filtration of structurally abnormal spermatozoa and reduced fertilization in teratospermic cats  

Microsoft Academic Search

Zona pellucida (ZP) penetration, in vitro fertilization, embryo development, and the morphology of fertilizing sperm were examined through use of normospermic (> 60% structurally normal sperm\\/ejaculate) versus teratospermic (< 40% structurally normal sperm\\/ejaculate) ejaculates from domestic cats. In addition, the effect of swim-up processing on sperm-oocyte interaction was compared with that of simple sperm washing. Normospermic and teratospermic ejaculates were

J. G. Howard; A. M. DONOGHUE; L. A. JOHNSTON; D. E. WILDT

1993-01-01

42

Protein kinase C plays an important role in the human zona pellucida- induced acrosome reaction  

Microsoft Academic Search

To investigate the involvement of protein kinases in signal transduction in the human zona pellucida (ZP)- induced acrosome reaction (AR), the effects of protein kinase (PK) activators, dibutyryl cAMP (PKA) and cGMP (PKG), phorbol 12-myristate 13-acetate (PMA, PKC), and the PKC inhibitor, staurosporine were studied. Sperm samples were obtained from normozoospermic men with normal sperm-ZP binding. Oocytes were obtained from

D. Y. Liu; H. W. G. Baker

1997-01-01

43

Effect of IVF and laser zona dissection on DNA methylation pattern of mouse zygotes  

Microsoft Academic Search

In vitro fertilization (IVF) and zona pellucida laser microdissection-facilitated IVF (Laser-IVF) are presently routine procedures\\u000a in human assisted reproduction. The safety of these methods at the epigenetic level is not fully understood. Studies on mouse\\u000a Laser-IVF embryos provide evidence that the use of Laser-IVF leads to reduced birth rate, indicating a potential harm of this\\u000a technique for the embryo. Hence,

Dominika D. Peters; Konstantin Lepikhov; Karsten Rodenacker; Susan Marschall; Auke Boersma; Peter Hutzler; Hagen Scherb; Jörn Walter; Martin Hrabé de Angelis

2009-01-01

44

Effect of a water-rich diet on adrenal zona glomerulosa in Gerbillus tarabuli.  

PubMed

The objective of this work is to evaluate the aldosterone level and to study the gerbil adrenal zona glomerulosa morphology in the presence of an important water diet content during seven days. Thirty adult gerbils, Gerbilus tarabuli, were randomly assigned to two watering regimes, with fifteen gerbils per group. The first group was the control and the second group was exposed to a water-rich diet during seven days. Thin sections of the adrenal glands were observed in photonic and electron microscopy. Their blood was sampled and analyzed for aldosterone, electrolyte concentrations and hematocrit. Urine was collected after 24hours. A water-rich diet decreased the body weight but increased the hematocrit, total protein, aldosterone concentration and urine volume. In electronic microscopy, the adrenal zona glomerulosa cells present a decrease in lipid compartment and Golgi apparatus zone development. The blood electrolyte concentrations were not changed significantly. These findings indicate that the water-rich diet exerts a potent stimulatory effect on aldosterone secretion by the gerbil adrenal zona glomerulosa through plasma volume perturbation. PMID:22325562

Saadi, Leila; Lebaili, Nemcha

2012-01-10

45

Autoimmune disease of the ovary induced by a ZP3 peptide from the mouse zona pellucida.  

PubMed Central

We describe a novel experimental system in mice for the study of ovarian autoimmune disease, a condition encountered in women with premature ovarian failure. The ovarian autoimmune disease is induced in B6AF1 mice by a 15-amino acid peptide (Cys-Ser-Asn-Ser-Ser-Ser-Ser-Gln-Phe-Gln-Ile-His-Gly-Pro-Arg) from mouse ZP3, the sperm-binding component of the zona pellucida that surrounds growing and mature oocytes. Whereas the peptide induces both T cell and antibody responses, adoptive transfer of CD4+ T cell lines derived from affected animals causes oophoritis without observable antibodies to the zona pellucida peptide. The primacy of the T cell response in the pathogenesis of disease is further substantiated by defining oophoritogenic peptides as small as eight amino acids (Asn-Ser-Ser-Ser-Ser-Gln-Phe-Gln) that do not elicit an antibody response to the full-length ZP3 peptide. The identification of a well characterized peptide as a causative agent of autoimmune oophoritis should facilitate understanding of the pathogenesis of this T cell-mediated autoimmune disease. Because the proteins of the zona pellucida are conserved among mammals (the mouse and human ZP3 proteins are 67% identical), this murine model may lead to better understanding of the pathogenesis of human autoimmune oophoritis. Images

Rhim, S H; Millar, S E; Robey, F; Luo, A M; Lou, Y H; Yule, T; Allen, P; Dean, J; Tung, K S

1992-01-01

46

Oocyte-specific expression of mouse Zp-2: developmental regulation of the zona pellucida genes.  

PubMed Central

The zona pellucida surrounds all mammalian oocytes and plays a vital role at fertilization and in early development. The genes that code for two of the mouse zona proteins (ZP2 and ZP3) represent a developmentally regulated set of genes whose expression serves as markers of mouse oocyte growth and differentiation. We previously characterized the single-copy Zp-3 gene and showed that its expression is oocyte specific and restricted to a narrow window of oocyte development. We now define the Zp-2 gene transcript and show that it is coordinately expressed with Zp-3 only during the 2-week growth phase of oogenesis that occurs prior to ovulation. Like Zp-3, the expression of Zp-2 is restricted to oocytes, and, although not detectable in resting oocytes, both ZP2 and ZP3 transcripts accumulate to become very abundant messengers in 50-microns-diameter oocytes. Ovulated eggs contain ZP2 and ZP3 transcripts which are 200 nucleotides shorter than those found in growing oocytes and have an abundance of less than 5% of the peak levels. In an attempt to understand the molecular details associated with the developmentally regulated, tissue-specific gene expression of the zona genes, the Zp-2 genetic locus has been characterized and its 5' flanking sequences have been compared with those of Zp-3. Both genes contain three short (8- to 12-base-pair) DNA sequences of 80 to 88% identity located within 250 base pairs of their transcription start sites. Images

Liang, L F; Chamow, S M; Dean, J

1990-01-01

47

Sperm Proteasomes Degrade Sperm Receptor on the Egg Zona Pellucida during Mammalian Fertilization  

PubMed Central

Despite decades of research, the mechanism by which the fertilizing spermatozoon penetrates the mammalian vitelline membrane, the zona pellucida (ZP) remains one of the unexplained fundamental events of human/mammalian development. Evidence has been accumulating in support of the 26S proteasome as a candidate for echinoderm, ascidian and mammalian egg coat lysin. Monitoring ZP protein degradation by sperm during fertilization is nearly impossible because those few spermatozoa that penetrate the ZP leave behind a virtually untraceable residue of degraded proteins. We have overcome this hurdle by designing an experimentally consistent in vitro system in which live boar spermatozoa are co-incubated with ZP-proteins (ZPP) solubilized from porcine oocytes. Using this assay, mimicking sperm-egg interactions, we demonstrate that the sperm-borne proteasomes can degrade the sperm receptor protein ZPC. Upon coincubation with motile spermatozoa, the solubilized ZPP, which appear to be ubiquitinated, adhered to sperm acrosomal caps and induced acrosomal exocytosis/formation of the acrosomal shroud. The degradation of the sperm receptor protein ZPC was assessed by Western blotting band-densitometry and proteomics. A nearly identical pattern of sperm receptor degradation, evident already within the first 5 min of coincubation, was observed when the spermatozoa were replaced with the isolated, enzymatically active, sperm-derived proteasomes. ZPC degradation was blocked by proteasomal inhibitors and accelerated by ubiquitin-aldehyde(UBAL), a modified ubiquitin protein that stimulates proteasomal proteolysis. Such a degradation pattern of ZPC is consistent with in vitro fertilization studies, in which proteasomal inhibitors completely blocked fertilization, and UBAL increased fertilization and polyspermy rates. Preincubation of intact zona-enclosed ova with isolated active sperm proteasomes caused digestion, abrasions and loosening of the exposed zonae, and significantly reduced the fertilization/polyspermy rates after IVF, accompanied by en-mass detachment of zona bound sperm. Thus, the sperm borne 26S proteasome is a candidate zona lysin in mammals. This new paradigm has implications for contraception and assisted reproductive technologies in humans, as well as animals.

Zimmerman, Shawn W.; Manandhar, Gaurishankar; Yi, Young-Joo; Gupta, Satish K.; Sutovsky, Miriam; Odhiambo, John F.; Powell, Michael D.; Miller, David J.; Sutovsky, Peter

2011-01-01

48

Sperm proteasomes degrade sperm receptor on the egg zona pellucida during mammalian fertilization.  

PubMed

Despite decades of research, the mechanism by which the fertilizing spermatozoon penetrates the mammalian vitelline membrane, the zona pellucida (ZP) remains one of the unexplained fundamental events of human/mammalian development. Evidence has been accumulating in support of the 26S proteasome as a candidate for echinoderm, ascidian and mammalian egg coat lysin. Monitoring ZP protein degradation by sperm during fertilization is nearly impossible because those few spermatozoa that penetrate the ZP leave behind a virtually untraceable residue of degraded proteins. We have overcome this hurdle by designing an experimentally consistent in vitro system in which live boar spermatozoa are co-incubated with ZP-proteins (ZPP) solubilized from porcine oocytes. Using this assay, mimicking sperm-egg interactions, we demonstrate that the sperm-borne proteasomes can degrade the sperm receptor protein ZPC. Upon coincubation with motile spermatozoa, the solubilized ZPP, which appear to be ubiquitinated, adhered to sperm acrosomal caps and induced acrosomal exocytosis/formation of the acrosomal shroud. The degradation of the sperm receptor protein ZPC was assessed by Western blotting band-densitometry and proteomics. A nearly identical pattern of sperm receptor degradation, evident already within the first 5 min of coincubation, was observed when the spermatozoa were replaced with the isolated, enzymatically active, sperm-derived proteasomes. ZPC degradation was blocked by proteasomal inhibitors and accelerated by ubiquitin-aldehyde(UBAL), a modified ubiquitin protein that stimulates proteasomal proteolysis. Such a degradation pattern of ZPC is consistent with in vitro fertilization studies, in which proteasomal inhibitors completely blocked fertilization, and UBAL increased fertilization and polyspermy rates. Preincubation of intact zona-enclosed ova with isolated active sperm proteasomes caused digestion, abrasions and loosening of the exposed zonae, and significantly reduced the fertilization/polyspermy rates after IVF, accompanied by en-mass detachment of zona bound sperm. Thus, the sperm borne 26S proteasome is a candidate zona lysin in mammals. This new paradigm has implications for contraception and assisted reproductive technologies in humans, as well as animals. PMID:21383844

Zimmerman, Shawn W; Manandhar, Gaurishankar; Yi, Young-Joo; Gupta, Satish K; Sutovsky, Miriam; Odhiambo, John F; Powell, Michael D; Miller, David J; Sutovsky, Peter

2011-02-23

49

Evaluation of bovine zona pellucida characteristics in polarized light as a prognostic marker for embryonic developmental potential.  

PubMed

It has previously been demonstrated that zona pellucida imaging of human oocytes using polarized light microscopy is a clinically applicable method for the noninvasive assessment of oocyte quality. This study was designed to investigate whether zona pellucida characteristics of bovine oocytes and zygotes in polarized light may similarly serve as a useful marker for developmental competence in bovine reproductive biotechnologies. Zona birefringence intensity parameters of 2862 oocytes/zygotes were objectively evaluated with an automatic analysis system and correlated with oocyte/zygote quality. In detail, immature oocytes of good quality assessed with brilliant cresyl blue staining showed significantly lower zona birefringence than poor-quality counterparts (P<0.001). After in vitro maturation and classification according to maturational status, the birefringence intensity parameters were significantly different in those oocytes that reached metaphase II compared with arrested stages (P<0.001). Following either parthenogenetic activation or IVF with subsequent in vitro culture in a well-of-the-well system until day 9, superior development as determined by cleavage, blastocyst formation, and hatching ability was associated with lower zona birefringence intensity parameters. When early zygote-stage embryos were selected and assorted in groups based on zona birefringence (high/medium/low), the group of embryos derived from high-birefringence zygotes displayed a significantly compromised developmental potential compared with low-birefringence zygotes. These results clearly show that developmentally competent bovine oocytes/zygotes exhibit lower zona birefringence intensity parameters. Therefore, birefringence imaging of zona pellucida is a suitable technique to predict bovine preimplantation embryo development. PMID:21415090

Koester, M; Mohammadi-Sangcheshmeh, A; Montag, M; Rings, F; Schimming, T; Tesfaye, D; Schellander, K; Hoelker, M

2011-03-17

50

Composición y propiedades cerámicas de las pizarras de la Zona Centro Ibérica del Macizo Ibérico Meridional (Norte de Jaén, España)  

Microsoft Academic Search

Composition and ceramic properties of shales from the Central-Iberican Zone of Southern Iberian Massif (Northern Jaen, Spain) The suitability of shale materials from the Iberian Massif in the Jaén province (Spain) to make ceramic materials has been eva- luated. The main components of these shales are illite, kaolinite, quartz and feldspars, with lesser amounts of chlorite, pyro- phyllite, paragonite and

M. VÁZQUEZ; J. PARRAS

51

Morfología, fenología y producción de biomasa aérea del Pithecellobium dulce, en una zona de monte espinoso tropical  

Microsoft Academic Search

The cover, morphology and aerial production of dry matter (DM) of the specie Pithececellebium dulce (Roxb.) Benth. was evaluated in a xerophyte area. The population density per hectare of trees higher and smaller than 1.5 m was 264 and 1935, equivalent to 22.6 and 2.1% of total botanical community, respectively. Ten adult trees higher than 1.5 m with similar dimension,

E. C. González; E. Chacón; J. Rodríguez

52

Degradación de Marismas Costera: el Impacto de la Eutrofización en la Supervivencia del las Marismas Costeras en New England y Central California, USA. (Salt Marsh Deterioration in New England and Central California: Impacts of Eutrophication on Salt Marsh Survival.)  

EPA Science Inventory

Las marismas costeras proporcionan numerosos beneficios a los ecosistemas, incluyendo la proporción del hábitat de la flora y fauna, la protección de las zonas costeras contra inundaciones durante eventos extremos, mejoran la calidad del agua para las almejas y ostras a través de...

53

'ZP domain' of human zona pellucida glycoprotein-1 binds to human spermatozoa and induces acrosomal exocytosis  

PubMed Central

Background The human egg coat, zona pellucida (ZP), is composed of four glycoproteins designated as zona pellucida glycoprotein-1 (ZP1), -2 (ZP2), -3 (ZP3) and -4 (ZP4) respectively. The zona proteins possess the archetypal 'ZP domain', a signature domain comprised of approximately 260 amino acid (aa) residues. In the present manuscript, attempts have been made to delineate the functional significance of the 'ZP domain' module of human ZP1, corresponding to 273-551 aa fragment of human ZP1. Methods Baculovirus-expressed, nickel-nitrilotriacetic acid affinity chromatography purified 'ZP domain' of human ZP1 was employed to assess its capability to bind and subsequently induce acrosomal exocytosis in capacitated human spermatozoa using tetramethyl rhodamine isothiocyanate conjugated Pisum sativum Agglutinin in absence or presence of various pharmacological inhibitors. Binding characteristics of ZP1 'ZP domain' were assessed employing fluorescein isothiocyanate (FITC) labelled recombinant protein. Results SDS-PAGE and immunoblot characterization of the purified recombinant protein (both from cell lysate as well as culture supernatant) revealed a doublet ranging from ~35-40 kDa. FITC- labelled 'ZP domain' of ZP1 binds primarily to the acrosomal cap of the capacitated human spermatozoa. A dose dependent increase in acrosomal exocytosis was observed when capacitated sperm were incubated with recombinant 'ZP domain' of human ZP1. The acrosome reaction mediated by recombinant protein was independent of Gi protein-coupled receptor pathway, required extra cellular calcium and involved both T- and L-type voltage operated calcium channels. Conclusions Results described in the present study suggest that the 'ZP domain' module of human ZP1 has functional activity and may have a role during fertilization in humans.

2010-01-01

54

Oxidative state and zona-binding ability in mouse spermatozoa treated with reduced glutathione  

Microsoft Academic Search

Background and Aims  Nuclear proteins in mature mammalian spermatozoa nuclei are oxidized to form numerous disulfide bonds. Reduced glutathione\\u000a (GSH) in the oocyte has been linked to spermatozoan nuclear decondensation after fertilization. In this study, we analyzed\\u000a whether GSH reduced protamines in sperm nuclei in vitro, and examined the zona-binding ability of treated nuclei.\\u000a \\u000a \\u000a \\u000a Methods  Three groups of mouse cauda epididymal sperma-tozoa

Kaoruko Mizuno; Shuji Hirata; Tsuyoshi Kasai; Seiichiro Nagai; Shoji Ohta; Kazuhiko Hoshi

2002-01-01

55

Are zona pellucida laser drilling and polar body biopsy safe for in vitro matured oocytes?  

Microsoft Academic Search

Introduction  Preconception diagnosis requires first polar body biopsy. When the hole in the zona pellucida is made with a laser beam, heat\\u000a propagation could, like the biopsy itself, be deleterious. Our aim was to evaluate the effect of this technique on human in vitro matured oocyte and embryo development.\\u000a \\u000a \\u000a \\u000a \\u000a Methods  One hunded fifty five retrieved immature oocytes from 75 women, matured in

Ibrahim Hammoud; Denise Molina-Gomes; Martine Albert; Marianne Bergere; Marc Bailly; Robert Wainer; Jacqueline Selva; Francois Vialard

2010-01-01

56

Dissociated dopaminergic neurons from substantia nigra zona compacta in young rats lack functional NMDA receptors  

Microsoft Academic Search

Glutamate-mediated excitotoxicity plays an important role in the degeneration of nigrostriatal dopamine (DA) neurons induced\\u000a by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), although the role of the N-methyl D-aspartate (NMDA) receptor subtype in this process is still uncertain. We studied glutamate receptor subtype agonist-induced\\u000a ionic currents in acutely dissociated DAergic neurons from the rat substantia nigra zona compacta (SNc) using the nystatin-perforated\\u000a patch-clamp whole-cell

J. Wu; L. Donald Partridge

1998-01-01

57

Dinámica de la descomposición de la hojarasca forestal en bosques de la cuenca del duero (provincia de zamora): Modelización de la pérdida de peso  

Microsoft Academic Search

Se ha estudiado la pérdida de peso en el proceso de descomposición de hojarasca en tres ecosistemas forestales de la zona semiárida Cuenca del Duero: un encinar climácico de Quercus rotundifolia Lam., un pinar paraclimácico de Pinus pinea L., y un pinar disclimácico de P. pinaster Ait. No se encuentran diferencias significativas entre la dinámica de descomposición de las hojas

I. M. Hernandez; I. Santa Regina; J. E Gallardo

1992-01-01

58

Relationship between sperm-zona pellucida binding assays and the 56-day nonreturn rate of cattle inseminated with frozen-thawed bull semen  

Microsoft Academic Search

Assays based on sperm-zona pellucida binding have been developed as diagnostic tests to predict the fertilizing potential of mammalian spermatozoa. Recently, we reported on the development of a sperm-zona pellucida binding assay (SZBA) for bull spermatozoa. The aim of the present study was to develop a hemi-zona assay (HZA) for bull spermatozoa and to investigate the relationship between SZBA and

A. R. Fazeli; B. R. Zhang; W. Steenweg; B. Larsson; M. M. Bevers; J. van den Broek; H. Rodriguez-Martinez; B. Colenbrander

1997-01-01

59

Contraceptive vaccines based on the zona pellucida glycoproteins for dogs and other wildlife population management.  

PubMed

Zona pellucida (ZP) glycoproteins, by virtue of their critical role in fertilization, have been proposed as candidate antigens for the development of contraceptive vaccines. In this review, the potential of a ZP-based contraceptive vaccine for the management of wildlife population, with special reference to street dogs, is discussed. Immunization of various animal species, including female dogs, with native porcine ZP led to inhibition of fertility, which was associated with the ovarian dysfunction. Immunization of female dogs with Escherichia coli-expressed recombinant dog ZP glycoprotein-3 (ZP3) either coupled to diphtheria toxoid or expressed as fusion protein with 'promiscuous' T non-B-cell epitope of tetanus toxoid also led to inhibition of fertility. To improve the contraceptive efficacy of ZP-based contraceptive vaccine, various groups are working on improving the immunogen, use of DNA vaccine as prime-boost strategy, and delivering the zona proteins/peptides presented on either virus-like particles or entrapped in microsphere. Host-specific live vectors such as ectromelia virus and cytomegalovirus have also been used to deliver mouse ZP3 in mice. Various studies show the enormous potential of the ZP-based vaccine for the management of wildlife population, where permanent sterilization may be desirable. PMID:21501280

Gupta, Satish K; Srinivasan, Villuppanoor A; Suman, Pankaj; Rajan, Sriraman; Nagendrakumar, Singanallur B; Gupta, Neha; Shrestha, Abhinav; Joshi, Pooja; Panda, Amulya K

2011-04-19

60

The human zona pellucida and scanning electron microscopy. Reality or artifacts?  

PubMed

The surface micro-morphology of the zona pellucida (ZP) was investigated in 158 inseminated but unfertilized mature human oocytes derived from assisted reproduction trials (ART) by means of traditional scanning electron microscopy (SEM) techniques (gold coating and conductive staining methods) and saponin-ruthenium red-osmium tetroxide-thiocarbohydrazide method (Sap-RR-Os-TC). The main aspect of the ZP by traditional SEM (122 oocytes) consisted in a porous, net-like structure (97 oocytes), whereas a nearly smooth or compact structure of ZP was detected in 25 oocytes (79.5% vs 20.5%). Using Sap RR-Os-TC method on 36 oocytes, 31 oocytes showed ZP with alternating tight and large meshed networks, whereas 5 oocytes displayed only tight meshed network (86.1% vs 13.9%). Due to our well standardized procedures, to the stabilizing action of the conductive staining on the zona material and similar results obtained with the use of Sap RR-Os-TC method, we confidentially regard the ZP changes, occurring in oocytes of various groups, as genuine features, likely related to their actual maturation status, rather than as artifacts. In addition, we emphasize the concept that a modern view of the ZP surface implies the best evidence of crossing filaments' network. We think that the ZP "spongy" or "compact" appearance is only the result of microfilaments network collapse, not the true three-dimensional (3-D) representation of ZP structure. PMID:11732593

Familiari, G; Relucenti, M; Ermini, M; Verlengia, C; Nottola, S A; Motta, P M

2001-01-01

61

A study of the zona piercing process in piezodriven intracytoplasmic sperm injection  

NASA Astrophysics Data System (ADS)

The mouse zona piercing process in piezodriven intracytoplasmic sperm injection (piezo-ICSI) is modeled and simulated with the material point method (MPM) to study the physics behind the piercing process. The MPM simulation reveals that the lateral vibration of the injection pipette plays a key role in the piercing process and disproves the claim that the axial displacement of the pipette pierces the zona. The lateral vibration of the injection pipette is further analyzed using the finite element method to investigate the role of mercury in the piercing process. It is shown that the use of mercury generally reduces the amplitude of the pipette tip and that a shorter piezopulse duration results in a smaller vibration amplitude. This implies that the effect of mercury on the piezo-ICSI procedure may lie in causing less oocyte damage due to the reduced amplitude of the pipette tip, and the development of a piezo-ICSI procedure without using mercury is possible by changing the dimensions of the injection micropipette and the piezodrill controller parameters.

Gan, Yong; Chen, Zhen

2008-08-01

62

Simulación numérica del transporte iónico en electrodeposición en condiciones más realísticas  

Microsoft Academic Search

El fenómeno de deposición electroquímica ramificada (ECD) en celdas delgadas presenta dos escalas que\\u000adifieren notablemente: una zona de casi neutralidad que ocupa la mayor parte del espacio y una capa límite\\u000ade carga varios órdenes de magnitud menor a aquélla. En la capa límite se produce la mayor parte de la\\u000acaída de potencial y de las variaciones de

Guillermo Santiago; Alejandro Peuriot; Guillermo Marshall; Fernando Molina

1970-01-01

63

Structural Analysis of Peptide-Analogues of Human Zona Pellucida ZP1 Protein with Amyloidogenic Properties: Insights into Mammalian Zona Pellucida Formation.  

PubMed

Zona pellucida (ZP) is an extracellular matrix surrounding and protecting mammalian and fish oocytes, which is responsible for sperm binding. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. These proteins polymerize into long interconnected filaments, through a common structural unit, known as the ZP domain, which consists of two domains, ZP-N and ZP-C. ZP is related in function to silkmoth chorion and in an evolutionary fashion to the teleostean fish chorion, also fibrous structures protecting the oocyte and embryo, that both have been proven to be functional amyloids. Two peptides were predicted as 'aggregation-prone' by our prediction tool, AMYLPRED, from the sequence of the human ZP1-N domain. Here, we present results from transmission electron microscopy, X-ray diffraction, Congo red staining and attenuated total reflectance Fourier-transform infrared spectroscopy (ATR FT-IR), of two synthetic peptide-analogues of these predicted 'aggregation-prone' parts of the human ZP1-N domain, that we consider crucial for ZP protein polymerization, showing that they both self-assemble into amyloid-like fibrils. Based on our experimental data, we propose that human ZP (hZP) might be considered as a novel, putative, natural protective amyloid, in close analogy to silkmoth and teleostean fish chorions. Experiments are in progress to verify this proposal. We also attempt to provide insights into ZP formation, proposing a possible model for hZP1-N domain polymerization. PMID:24069181

Louros, Nikolaos N; Iconomidou, Vassiliki A; Giannelou, Polina; Hamodrakas, Stavros J

2013-09-12

64

Structural Analysis of Peptide-Analogues of Human Zona Pellucida ZP1 Protein with Amyloidogenic Properties: Insights into Mammalian Zona Pellucida Formation  

PubMed Central

Zona pellucida (ZP) is an extracellular matrix surrounding and protecting mammalian and fish oocytes, which is responsible for sperm binding. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. These proteins polymerize into long interconnected filaments, through a common structural unit, known as the ZP domain, which consists of two domains, ZP-N and ZP-C. ZP is related in function to silkmoth chorion and in an evolutionary fashion to the teleostean fish chorion, also fibrous structures protecting the oocyte and embryo, that both have been proven to be functional amyloids. Two peptides were predicted as ‘aggregation-prone’ by our prediction tool, AMYLPRED, from the sequence of the human ZP1-N domain. Here, we present results from transmission electron microscopy, X-ray diffraction, Congo red staining and attenuated total reflectance Fourier-transform infrared spectroscopy (ATR FT-IR), of two synthetic peptide-analogues of these predicted ‘aggregation-prone’ parts of the human ZP1-N domain, that we consider crucial for ZP protein polymerization, showing that they both self-assemble into amyloid-like fibrils. Based on our experimental data, we propose that human ZP (hZP) might be considered as a novel, putative, natural protective amyloid, in close analogy to silkmoth and teleostean fish chorions. Experiments are in progress to verify this proposal. We also attempt to provide insights into ZP formation, proposing a possible model for hZP1-N domain polymerization.

Louros, Nikolaos N.; Iconomidou, Vassiliki A.; Giannelou, Polina; Hamodrakas, Stavros J.

2013-01-01

65

Specific activation requirements of zona-free sheep oocytes before and after somatic cell nuclear transfer.  

PubMed

In this study, the effect of the steps involved in zona-free somatic cell nuclear transfer (SCNT) on oocyte transcripts was investigated in sheep. To establish the reliable combined electrical-chemical activation for zona-free oocytes, oocytes were first exposed to an electrical pulse and then treated with 18 chemical activation regimens designed through modifying duration and concentration of ionomycin and 6-dimethyl aminopurine (6-DMAP), which is routinely used for SCNT. Electrofusion-mediated nuclear transfer significantly reduced transcript abundances of CCNB1, POU5F1, NPM2, GMMN, and CX43 compared to intact oocytes. Maximum parthenogenetic blastocyst development was obtained when oocytes were submitted to electric pulse and then to (1) 5??M ionomycin for 5 or 2.5?min, both followed by 2?h of incubation with 6-DMAP (41.7±1.1, and 42.4±1.4%, respectively), (2) 5??M ionomycin for 1?min+6-DMAP for 4?h (43.1±1.4%), and (3) 2.5??M ionomycin for 1?min+6-DMAP for 2?h (42.4±1.4%), with significant differences compared to all the other groups. Statistical assessment of interactions between duration and concentration of ionomycin and duration of 6-DMAP exposure revealed that (1) concentration of ionomycin may be a more important factor than its duration, (2) both a long exposure period and a low concentration of ionomycin had marked decreasing effects on parthenogenetic development of zona-free oocytes, and (3) high duration of exposure to 6-DMAP can reduce parthenogenetic development. Despite an activation preference of parthenogenetic oocytes, a significantly higher rate of cloned blastocyst development was observed when reconstructed oocytes were activated with 5??M ionomycin for 5?min rather than 2.5??M ionomycin for 1?min (8.8±2.5 vs. 1.25±2.2%). These results suggested that SCNT steps have determining effects on oocyte transcripts and activation preferences of the reconstituted oocytes compared to intact counterparts. In this sense, reconstituted oocytes may need a higher concentration of ionomycin for a longer period than intact oocytes. PMID:23713434

Hajian, M; Kiani, M; Hosseini, M S; Ostadhosseini, S; Forouzanfar, M; Afrough, M; Nasr-Esfahani, M H

2013-06-01

66

Rat adrenal zona fasciculata cell electrophysiological responses to ACTH and gamma-MSH vary with age.  

PubMed

A comparison was made of the resting membrane potential (MP) as well as the MP responses to K+, ACTH and gamma-MSH of superfused adrenocortical, zona fasciculata cells from young (Y) and old (O) male rats. The resting MP of these cells did not vary with age. However, the MP responses to altered extracellular [K+] varied significantly with age. A prolonged biphasic depolarization was observed following ACTH administration; these responses were significantly reduced in O cells. In contrast, stimulation with gamma-MSH did not consistently elicit depolarization in the Y but caused a consistent and prominent depolarization in O cells. These findings suggest that aging is associated with changes in primary membrane events which could explain the reduced ACTH-stimulated steroidogenesis associated with age. Elevated cellular responses to gamma-MSH may contribute to a maintenance of, or increase in, circulating corticosterone levels in aged rats. PMID:2849700

Lymangrover, J R; Valego, N K; Shilling, R D

1988-06-01

67

Comparison of the results of human embryo biopsy and outcome of PGD after zona drilling using acid Tyrode medium or a laser  

Microsoft Academic Search

BACKGROUND: Zona pellucida opening for blastomere removal can be done by mechanical or chemical means, or by laser. So far, only limited data on the use of laser systems for zona drilling in cases of PGD are available. METHODS: Results of embryo biopsy and outcome of PGD in two periods were compared. In the first period, acid Tyrode medium was

H. Joris; A. De Vos; R. Janssens; P. Devroey; I. Liebaers; A. Van Steirteghem

2003-01-01

68

INDUCTION OF ZONA RADIATA PROTEINS AND VITELLOGENINS IN ESTRADIOL AND NONYLPHENOL EXPOSED MALE SHEEPSHEAD MINNOWS (CYPRINODON VARIEGATUS)  

EPA Science Inventory

Knoebl, Iris, Michael J. Hemmer and Nancy D. Denslow. 2004. Induction of Zona Radiata Proteins and Vitellogenins in Estradiol and Nonylphenol Exposed Male Sheepshead Minnows (Cyprinodon variegatus). Mar. Environ. Res. 58(2-5):547-551. (ERL,GB X1059). Several genes normall...

69

Effects of angiotensin II and AIII microinjections into the zona incerta after intra- and extracellular fluid loss  

Microsoft Academic Search

Our recent results showed that angiotensin II or III (AII, AIII) microinjected into the zona incerta (ZI) significantly increased water intake. The most effective doses of AII and AIII were also defined. The two neuropeptides had their effects differently on drinking via different receptors. AII bound to AT1 that was blocked by AT1 receptor antagonist Losartan and the effect of

Éva Eszter Bagi; Éva Fekete; Dániel Bányai; László Lénárd

2004-01-01

70

Effects of White Blood Cells on the In Vitro Penetration of Zona-free Hamster Eggs by Human Spermatozoa  

Microsoft Academic Search

The presence of white blood cells in semen has been associated with male infertility. Previous studies indi- cate that pyospermia occurs in conjunction with de- creases in sperm motility, number of normal sperm forms, and penetration rates in the zona-free hamster egg sperm penetration assay. We have evaluated the relationship of seminal white blood cells and sperm function, as reflected

DONALD K. MARUYAMA; RALPH W. HALE; B. JANE ROGERS

71

A novel treatment strategy for ovarian cancer based on immunization against zona pellucida protein (ZP) 3.  

PubMed

We tested the principle of treating malignant ovarian tumors by vaccination against their ectopically expressed protein, zona pellucida glycoprotein (ZP) 3, using as the experimental model the granulosa cell tumors that develop in transgenic mice expressing the simian virus 40 T-antigen under the inhibin-? promoter (inh?/Tag). We found high ZP3 expression in granulosa cell tumors of the transgenic mice, in human surface ovarian cancer and granulosa cell lines, and in human granulosa cell tumors and their metastases. Early preventive immunization (between 2 and 5.5 mo of age) of transgenic mice with recombinant human (rh) ZP3 prevented ovarian tumorigenesis, and delayed therapeutic immunization (between 4.5 and 7 mo) reduced weights of existing tumors by 86 and 75%, respectively (P<0.001), compared to vehicle-treated control mice. No objective side effects of the immunizations were observed. Liver metastases were found in nontreated/vehicle-treated controls (n=7/39), but none following active rhZP3 immunizations (n=0/36; P<0.05). Immunization with rhZP3 was highly effective, as demonstrated by the induction of anti-ZP3 antibodies, as well as proliferative responses to the ZP3 antigen. These results signal rhZP3 immunization as a novel strategy to be developed for the immunotherapy of ovarian granulosa cell tumors, as well as for that of other malignancies that may express ZP3. PMID:21974931

Rahman, Nafis A; Bennink, Herjan J T Coelingh; Chrusciel, Marcin; Sharp, Victoria; Zimmerman, Yvette; Dina, Roberto; Li, Xiangdong; Ellonen, Antti; Rivero-Müller, Adolfo; Dilworth, Stephen; Ghaem-Maghami, Sadaf; Vainio, Olli; Huhtaniemi, Ilpo

2011-10-05

72

Evidence for extracellular, but not intracellular, generation of angiotensin II in the rat adrenal zona glomerulosa  

SciTech Connect

Based on the observation that high levels of renin and angiotensin II (Ang II) are found in the adrenal zona glomerulosa (ZG), it has been postulated that Ang II is formed intracellularly by the renin-converting enzyme cascade in this tissue. To test this hypothesis, the authors examined renin-angiotensin system components in subcellular fractions of the rat adrenal ZG. Renin activity and immunoreactive-Ang II (IR-Ang II) were observed in vesicular fractions but were not colocalized. In addition, angiotensinogen, angiotensin I, and converting enzyme were not observed in the renin or IR-Ang II-containing vesicular fractions. These data do not support the hypothesis that Ang II is formed intracellularly within the renin-containing vesicles of the ZG. Rather, since modulatable renin release from adrenal ZG slices was observed and renin activity was found in dense vesicular fractions (33-39% sucrose), it is likely that Ang II formation in the ZG is extracellular and initiated by the release of vesicular renin. In ZG lysomal fractions {sup 125}I-labeled Ang II was degraded to {sup 125}I-labeled des-(Phe{sup 8})Ang II. Since Ang II antibodies do not recognize des-(Phe{sup 8})Ang II, these finding explain why IR-Ang II in the ZG is due predominantly to Ang II and not to its C-terminal immunoreactive fragments.

Urata, H.; Khosla, M.C.; Bumpus, M.; Husain, A. (Research Institute of the Cleveland Clinic Foundation, OH (USA))

1988-11-01

73

Corticotropin-inhibiting peptide enhances aldosterone secretion by dispersed rat zona glomerulosa cells.  

PubMed

Corticotropin-inhibiting peptide (CIP), the 7-38 fragment of human ACTH(1-39), is known to act as an antagonist of ACTH receptors. Accordingly, CIP has been found to inhibit ACTH-stimulated glucocorticoid secretion of dispersed rat adrenocortical cells, without per se affecting the basal production. We confirmed these findings, but unexpectedly observed that CIP concentration-dependently raised basal aldosterone secretion from fresh suspensions of rat zona glomerulosa (ZG) cells, maximal effective concentration being 10(-6) M. CIP (10(-6) M) partially reversed the ZG-cell response to ACTH, but not to the Ca2+-dependent agonists angiotensin-II (ANG-II) and K+. The aspecific ANG-II-receptor antagonist saralasin (10(-6) M) blocked the aldosterone response of ZG cells to 10(-6) M CIP, and in the presence of the Ca2+-channel blocker verapamil CIP was ineffective. Collectively, these findings suggest that CIP enhances aldosterone secretion of rat ZG through a mechanism involving the activation of ANG-II receptors and the consequent rise in the cytosolic Ca2+ concentration. They also stress that this side-effect of CIP must be taken into account in interpreting the results of investigations on the adrenal cortex, where CIP has been employed as an ACTH-receptor antagonist. PMID:9877215

Malendowicz, L K; Rebuffat, P; Nussdorfer, G G; Nowak, K W

1998-10-01

74

Zona Pellucida Domain-Containing Protein ?-Tectorin is Crucial for Zebrafish Proper Inner Ear Development  

PubMed Central

Background The zona pellucida (ZP) domain is part of many extracellular proteins with diverse functions from structural components to receptors. The mammalian ?-tectorin is a protein of 336 amino acid residues containing a single ZP domain and a putative signal peptide at the N-terminus of the protein. It is 1 component of a gel-like structure called the tectorial membrane which is involved in transforming sound waves into neuronal signals and is important for normal auditory function. ?-Tectorin is specifically expressed in the mammalian and avian inner ear. Methodology/Principal Findings We identified and cloned the gene encoding zebrafish ?-tectorin. Through whole-mount in situ hybridization, we demonstrated that ?-tectorin messenger RNA was expressed in the otic placode and specialized sensory patch of the inner ear during zebrafish embryonic stages. Morpholino knockdown of zebrafish ?-tectorin affected the position and number of otoliths in the ears of morphants. Finally, swimming behaviors of ?-tectorin morphants were abnormal since the development of the inner ear was compromised. Conclusions/Significance Our results reveal that zebrafish ?-tectorin is specifically expressed in the zebrafish inner ear, and is important for regulating the development of the zebrafish inner ear. Lack of zebrafish ?-tectorin caused severe defects in inner ear formation of otoliths and function.

Yang, Chung-Hsiang; Cheng, Chia-Hsiung; Chen, Gen-Der; Liao, Wei-Hao; Chen, Yi-Chung; Huang, Kai-Yun; Hwang, Pung-Pung; Hwang, Sheng-Ping L.; Huang, Chang-Jen

2011-01-01

75

Delineation of downstream signalling components during acrosome reaction mediated by heat solubilized human zona pellucida  

PubMed Central

Background Human egg is enveloped by a glycoproteinaceous matrix, zona pellucida (ZP), responsible for binding of the human spermatozoa to the egg and induction of acrosomal exocytosis in the spermatozoon bound to ZP. In the present manuscript, attempts have been made to delineate the downstream signalling components employed by human ZP to induce acrosome reaction. Methods Heat-solubilized human ZP (SIZP) was used to study the induction of acrosome reaction in capacitated human spermatozoa using tetramethylrhodamine isothiocyanate conjugated Pisum sativum agglutinin (TRITC-PSA) in absence or presence of various pharmacological inhibitors. In addition, intracellular calcium ([Ca2+]i) levels in sperm using Fluo-3 acetoxymethyl ester as fluorescent probe were also estimated in response to SIZP. Results SIZP induces acrosomal exocytosis in capacitated human sperm in a dose dependent manner accompanied by an increase in [Ca2+]i. Human SIZP mediated induction of acrosome reaction depends on extracellular Ca2+ and involves activation of Gi protein-coupled receptor, tyrosine kinase, protein kinases A & C and phosphoinositide 3 (PI3)- kinase. In addition, T-type voltage operated calcium channels and GABA-A receptor associated chloride (Cl-) channels play an important role in SIZP mediated induction of acrosome reaction. Conclusions Results described in the present study provide a comprehensive account of the various downstream signalling components associated with human ZP mediated acrosome reaction.

2010-01-01

76

Vitellogenin and zona radiata proteins as biomarkers of endocrine disruption in peregrine falcon (Falco peregrinus).  

PubMed

The aim of this study was to test a specific method for the detection of Vitellogenin (Vtg) and Zona Radiata Proteins (Zrp) in plasma from peregrine falcon (Falco peregrinus) as specific biomarkers for the evaluation of the effects of endocrine disruptors. The method was assayed with different peregrine falcon individuals (including mature and immature birds of both sexes) from a Spanish population being studied in terms of their contamination with organochlorine compounds with endocrine disrupting properties. This study shows that mouse anti bird Vtg monoclonal antibody ND3C3 (Biosense) seems to be the most specific antibody in binding plasmatic lipoproteins in peregrine falcon when compared to other anti Vtg antibodies. Rabbit anti salmon Zrp polyclonal antibodies O146 (Biosense) show cross-reactivity with Zrp in the samples studied. These preliminary results confirm the applicability of both of these diagnostic tools assayed (induction of Vtg and Zrp) in detecting exposure to Endocrine Disrupting Chemicals (EDCs) in this species. The increase of Vtg and Zrp detected in male specimens suggest a potential hazard to EDCs in the peregrine falcon which represents a species still affected by organochlorine compounds, and in particular those with estrogenic activity. PMID:17223181

Jiménez, Begoña; Mori, Gabriele; Concejero, Miguel A; Merino, Rubén; Casini, Silvia; Fossi, María Cristina

2007-01-16

77

Identification of potential oviductal factors responsible for zona pellucida hardening and monospermy during fertilization in mammals.  

PubMed

Oviduct fluid increases the time required for digestion of the zona pellucida (ZP) by proteolytic enzymes (ZP hardening). This effect has been associated with levels of monospermy after in vitro fertilization (IVF) in the pig and cow, but the possible existence of a directly proportional relationship between hardening and monospermy remains unknown. To investigate whether variations in hardening of different oviductal fluids (OFs) are correlated with variations in levels of monospermy after IVF, porcine oocytes were incubated with three batches of OFs known to produce different ZP hardening effects (3, 7, and 25 min); after IVF, monospermy levels were 0%, 14.58% ± 5.14%, and 35.14% ± 7.95%, respectively. These results could partially explain the lack of polyspermy found during in vivo fertilization in pigs (with a hardened oviductal ZP) compared with levels found during IVF (with no hardened ZP). Using the bovine model, OF was fractionated by heparin affinity chromatography, and the hardening effect on the ZP was tested for each fraction obtained from a linear gradient of sodium chloride concentration. The highest effect was obtained with the fraction eluted with 0.4 M sodium chloride. Fractions with high-level or low-level effects were processed by on-chip electrophoresis and high-performance liquid chromatography-tandem mass spectrometry. A list of potential proteins responsible for this effect includes OVGP1 and members of the HSP and PDI families. PMID:23863406

Mondéjar, Irene; Martínez-Martínez, Irene; Avilés, Manuel; Coy, Pilar

2013-09-27

78

American cutaneous leishmaniasis in Pernambuco, Brazil: eco-epidemiological aspects in 'Zona da Mata' region.  

PubMed

American cutaneous leishmaniasis (ACL) has been increasing in Pernambuco, thus becoming an important problem for Public Health. The incindence is predominant in the region called 'Zona da Mata', in the east of this state. This region corresponds geographically to the primitive area of the Atlantic forest. In order to characterize the eco-epidemiology expression of ACL in this region, two localities situated in the municipalities of Amaraji e Cortes have been selected by the criterion of higher incindence of human cases. Five stocks of patients were characterized and identified on the basis of enzyme profiles as a new variant of Leishmania (V.) braziliensis. A survey of wild and domestic animals was carried out by means of a parasitological and serological diagnosis. Through the analysis of the spleen and liver imprints, were detected amastigotes compatible with Leishmania in five Nectomys s. squamipes, five Bolomys 1. pixuna, two Rattus r. alexandrinus and one Rattus r. frugivorus. For two years we carried out monthly sandflies captures using CDC light traps as well as manual captures. Lutzomyia whitmani was predominant, which accounted for 97.4% of the total. These data indicate a strong evidence on the vector and the potential reservoirs of L. braziliensis in this region. PMID:7476230

Brandão-Filho, S P; de Carvalho, F G; de Brito, M E; Almeida, F de A; Nascimento, L A

79

Estimating Young's modulus of zona pellucida by micropipette aspiration in combination with theoretical models of ovum  

PubMed Central

The zona pellucida (ZP) is the spherical layer that surrounds the mammalian oocyte. The physical hardness of this layer plays a crucial role in fertilization and is largely unknown because of the lack of appropriate measuring and modelling methods. The aim of this study is to measure the biomechanical properties of the ZP of human/mouse ovum and to test the hypothesis that Young's modulus of the ZP varies with fertilization. Young's moduli of ZP are determined before and after fertilization by using the micropipette aspiration technique, coupled with theoretical models of the oocyte as an elastic incompressible half-space (half-space model), an elastic compressible bilayer (layered model) or an elastic compressible shell (shell model). Comparison of the models shows that incorporation of the layered geometry of the ovum and the compressibility of the ZP in the layered and shell models may provide a means of more accurately characterizing ZP elasticity. Evaluation of results shows that although the results of the models are different, all confirm that the hardening of ZP will increase following fertilization. As can be seen, different choices of models and experimental parameters can affect the interpretation of experimental data and lead to differing mechanical properties.

Khalilian, Morteza; Navidbakhsh, Mahdi; Valojerdi, Mojtaba Rezazadeh; Chizari, Mahmoud; Yazdi, Poopak Eftekhari

2010-01-01

80

Protein phosphorylation regulates the mouse sperm acrosome reaction induced by the zona pellucida.  

PubMed

Recently, the ligand-receptor signal transduction mechanism has been implicated in mediating the zona pellucida (ZP)-induced acrosome reaction. Little is known about the role of protein phosphorylation in this specific event. We examine whether modification of protein phosphorylation and dephosphorylation affects the kinetics of the acid-solubilized ZP-induced acrosome reaction of mouse sperm. Mouse epididymal sperm were incubated in modified Krebs-Ringer bicarbonate medium for a period of 90 to 120 min and then treated with 2 acid-solubilized ZP/microliters for an additional 60 min. The chlortetracycline fluorescence assay was used to monitor the acrosome reaction. Capacitated sperm were inhibited from undergoing acid-solubilized ZP-induced acrosome reaction in the presence of an inhibitor of cyclic nucleotide-dependent protein kinase, H8; activators of the Ca(2+)- and phospholipid-dependent protein kinase (protein kinase C); an inhibitor of phosphatases 1 and 2A, okadaic acid; or an inhibitor of protein tyrosine kinases, genistein. The addition of inhibitors of protein kinase C, such as staurosporine, H7, and protein kinase C [19-36] pseudosubstrate, inhibited the phorbol ester-dependent inhibition of the acid-solubilized ZP-induced acrosome reaction. The present study suggests that protein phosphorylation and dephosphorylation play a regulatory role in the process of the ZP-induced acrosome reaction. PMID:1335314

Furuya, S; Endo, Y; Oba, M; Matsui, Y; Nozawa, S; Suzuki, S

1992-08-01

81

Effects of nonylphenol on aldosterone release from rat zona glomerulosa cells.  

PubMed

Alkylphenol ethoxylate, which consists of approximately 80% nonylphenol ethoxylate (NPE), is a major nonionic surfactant. Nonylphenol (NP), the primary degradation product of NPE, has been reported to interfere with reproduction in fish, reptiles, and mammals by inducing cell death in the gonads and by affecting other reproductive parameters. However, the effects of NP on rat adrenal zona glomerulosa cells (ZG) and the underlying mechanisms remain unclear. In this study, we explored the effects of NP on aldosterone release. ZG cells were incubated with NP in the presence or absence of the secretagogues angiotensin II (ANG II), potassium, 8-Br-cAMP, 25-OH-cholesterol, corticosterone or cyclopiazonic acid (CPA). After performing radioimmunoassay (RIA) and Western blot analysis, we found that (1) NP stimulated aldosterone release in cells induced by ANG II, KCl, 8-Br-cAMP, 25-OH-cholesterol, corticosterone, and CPA; (2) NP triggered the release of higher amounts of pregnenolone in cells treated with vehicle and 25-OH-cholesterol+trilostane than in cells treated with other compounds; and (3) the stimulatory effect of NP seemed to be mediated through steroidogenic acute regulatory protein (StAR) and aldosterone synthase activity. These observations suggest that the effects of NP are mediated via increased free Ca(2+) in the cytoplasm. PMID:22001352

Chang, Ling-Ling; Wun, Wan-Song Alfred; Wang, Paulus S

2011-10-04

82

Effects of dehydroepiandrosterone on aldosterone release in rat zona glomerulosa cells.  

PubMed

The present study was to investigate the effects and action mechanisms of dehydroepiandrosterone (DHEA) on steroidogenesis in rat adrenal zona glomerulosa cells (ZG). ZG cells were incubated with DHEA in the presence or absence of angiotensin II (AngII), a high concentration of potassium, 8-Br-cAMP, forskolin, 25-OH-cholesterol, pregnenolone, progesterone, deoxycorticosterone, corticosterone, A23187, or cyclopiazonic acid (CPA) at 37 degrees C for 1 h. The concentration of aldosterone or pregnenolone in the culture medium was then measured by radioimmunoassay (RIA). The cells were used to determine the cellular cAMP content. The data demonstrated that: (1) DHEA inhibited AngII-, high concentration of KCl-, forskolin-, 8-Br-cAMP-, 25-OH-cholesterol-, pregnenolone-, progesterone-, deoxycorticosterone-, corticosterone-, A23187-, or CPA-stimulated aldosterone release; (2) DHEA increased 25-OH-cholesterol-stimulated pregnenolone release but not when 25-OH-cholesterol was combined with trilostane; (3) DHEA noncompetitively inhibited aldosterone synthase but showed uncompetitive inhibition of P450scc. These results suggest that DHEA acts directly on rat ZG cells to diminish aldosterone secretion by inhibition of a post-cAMP pathway or by acting on intracellular Ca2+ mobilization. In addition it affects the function of post-P450scc steroidogenic enzymes. PMID:18324480

Chang, Ling-Ling; Wun, Wan-Song Alfred; Wang, Paulus S

2008-03-07

83

Effects of angiotensin II on the zona fasciculata of the rat adrenal cortex: an ultrastructural stereologic study.  

PubMed Central

The effects of a chronic administration of angiotensin II on the zona fasciculata of rats treated with dexamethasone and maintenance doses of ACTH were investigated by morphometric methods applied to electron microscopy. It was found that angiotensin induced a significant increase in the volume of cells, nuclei, mitochondrial and lipid compartments as well as in the surface area of smooth endoplasmic reticulum and mitochondrial cristae. Noticeable also were the hypertrophy of the Golgi apparatus and the increase in the number of acid phosphatase-positive dense bodies. These findings are interpreted as indicating that the renin-angiotensin system is involved in the stimulation of the growth and steroidogenic capacity of the rat zona fasciculata. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4

Nussdorfer, G G; Robba, C; Mazzochi, G; Rebuffat, P

1981-01-01

84

The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa: differences from the zona pellucida  

Microsoft Academic Search

The acrosome reaction (AR), an absolute requirement for spermatozoa and egg fusion, requires the influx of Ca2+ into the spermatozoa through voltage-dependent Ca2+ channels and store-operated channels. Maitotoxin (MTx), a Ca2+-mobilizing agent, has been shown to be a potent inducer of the mouse sperm AR, with a pharmacology similar to that of the zona pellucida (ZP), possibly suggesting a common

Julio C Chávez; Gerardo A de Blas; José L de la Vega-Beltrán; Takuya Nishigaki; Mayel Chirinos; María Elena González-González; Fernando Larrea; Alejandra Solís; Alberto Darszon; Claudia L Treviño

2011-01-01

85

Effect of genistein on acrosome reaction and zona pellucida binding independent of protein tyrosine kinase inhibition in bull  

Microsoft Academic Search

Aim:To investigate if the phytoestrogen, genistein, affects essential functions of cryopreserved bovine spermatozoa.Methods:The effect of genistein upon motility was assessed by computer-assisted motion analysis. Hemizona assay was performed to detect the ability of spermatozoa binding to the zona pellucida. The inducibility of the acrosome reaction using progesterone and ZP3-6 peptide was analysed by fluorescein-conjugated Pisum sativum agglutinin (FITC-PSA)\\/Hoechst 33258 double

Viviana A. Menzel; Elvira Hinsch; Wolfgang Hägele; Klaus-Dieter Hinsch

2007-01-01

86

Activation by Angiotensin II of Ca 2+ -dependent K + and Cl ? Currents in Zona Fasciculata Cells of Bovine Adrenal Gland  

Microsoft Academic Search

.   The effects of angiotensin II (100 nm) on the electrical membrane properties of zona fasciculata cells isolated from calf adrenal gland were studied using the\\u000a whole cell patch recording method. In current-clamp condition, angiotension II induced a biphasic membrane response which\\u000a began by a transient hyperpolarization followed by a depolarization more positive than the control resting potential. These\\u000a effects

A. Chorvatova; A. Guyot; C. Ojeda; O. Rougier; A. Bilbaut

1998-01-01

87

Characterization of cell surface polypeptides of unfertilized, fertilized, and protease-treated zona-free mouse eggs  

SciTech Connect

The polypeptide composition of unfertilized, fertilized, and protease-treated zona-free mouse eggs was evaluated in this study. Zona-free eggs were radioiodinated by an Iodogen-catalyzed reaction. Light microscopic autoradiography of egg sections revealed that labeling was restricted to the cell surface. Labeled eggs were solubilized, and cell surface polypeptides were identified by one-dimensional SDS polyacrylamide gel electrophoresis and autoradiography. The unfertilized egg demonstrated 8-10 peptides that incorporated {sup 125}I, with major bands observed at approximately 145-150, 94, and 23 kilodaltons (kD). Zona-free eggs fertilized in vitro and then radiolabeled demonstrated several new bands in comparison to unfertilized eggs, with a major band appearing at approximately 36 kD. Treatment of radiolabeled unfertilized eggs with either trypsin or chymotrypsin (1 mg/ml for 5-20 min) caused enzyme-specific modifications in labeled polypeptides. Trypsin (T) treatment resulted in time-dependant modification of the three major peptides at 145-150, 94, and 23 kD. Chymotrypsin (CT) treatment, in contrast, was associated with loss or modification of the 94 kD band, with no apparent effect on either the 145-150 or 23 kD band. Taken together with previous data indicating that T or CT egg treatment interferes with sperm-egg attachment and fusion, these results suggest a possible role for the 94 kD protein in sperm-egg interaction.

Boldt, J.; Gunter, L.E.; Howe, A.M. (Medical College of Georgia, Augusta (USA))

1989-05-01

88

O2 dependence of pregnenolone and aldosterone synthesis in mitochondria from bovine zona glomerulosa cells.  

PubMed

Hypoxia in vivo results in a decrease in aldosterone not accounted for by extra-adrenal controllers. We have demonstrated that aldosteronogenesis but not cortisol synthesis in the whole cell is O2 sensitive. In the intact glomerulosa cell, this sensitivity is located in the late pathway step catalyzed by conversion of corticosterone to aldosterone (P-450aldo), whereas the early pathway catalyzed by conversion of cholesterol to pregnenolone (P-450scc) is not inhibited until PO2 is very low. Because P-450aldo and P-450scc are mitochondrial enzymes that depend on the same NADPH-specific electron transport proteins, we hypothesized that O2 sensitivity would be independent of energy production and expressed in isolated mitochondria. We measured the conversion of exogenous 25(OH)-cholesterol to pregnenolone and of exogenous corticosterone to aldosterone in the presence of cyanoketone in mitochondria isolated from bovine zona glomerulosa cells and exposed to an experimental gas (1-100% O2) vs. a room air control. Pregnenolone production was not affected until PO2 was < 35 Torr and decreased to almost nil when PO2 was < 30 Torr. In contrast, aldosterone production increased under hyperoxia and decreased under moderate decreases in O2. The conversion of corticosterone to aldosterone was maintained at approximately 50% of control, even when PO2 was < 20 Torr. The sensitivity of the aldosterone pathway to changes in O2 within the physiological range appears to reside in the mitochondrial late pathway (i.e., P-450aldo) and is not significantly influenced by cytosolic regulators of steroidogenesis or by limitation of reducing equivalents. PMID:7649895

Raff, H; Jankowski, B

1995-05-01

89

Mapping of minimal motifs of B-cell epitopes on human zona pellucida glycoprotein-3.  

PubMed

The human zona pellucida glycoprotein-3 (hZP3) by virtue of its critical role during fertilization has been proposed as a promising candidate antigen to develop a contraceptive vaccine. In this direction, it is imperative to map minimal motifs of the B cell epitopes (BCEs) so as to avoid ZP-specific oophoritogenic T cell epitopes (TCEs) in the ZP3-based immunogens. In this study, based on known results of mapping marmoset and bonnet monkey ZP3 (mstZP3 and bmZP3), two predictable epitopes(23-30??and??301-320) on hZP3 were first confirmed and five minimal motifs within four epitopes on hZP3 were defined using serum to recombinant hZP3a(22-176) or hZP3b(177-348) as well as a biosynthetic peptide strategy. These defined minimal motifs were QPLWLL(23-28) for hZP3(23-30), MQVTDD(103-108) for hZP3(93-110), EENW(178-181) for hZP3(172-190), as well as SNSWF(306-310) and EGP(313-315) for hZP3(301-320), respectively. Furthermore, the antigenicity of two peptides for hZP3(172-187) and hZP3(301-315) and specificity of the antibody response to these peptides were also evaluated, which produced high-titer antibodies in immunized animals that were capable of reacting to ZP on human oocytes, r-hZP3b(177-348) protein, as well as r-hZP3(172-190), r-hZP3(303-310), and r-hZP3(313-320) epitope peptides fused with truncated GST188 protein. PMID:22162720

Xu, Wan-Xiang; He, Ya-Ping; Wang, Jian; Tang, Hai-Ping; Shi, Hui-Juan; Sun, Xiao-Xi; Ji, Chao-Neng; Gu, Shao-Hua; Xie, Yi

2011-11-17

90

Antinociceptive effect of stimulating the zona incerta with glutamate in rats.  

PubMed

The zona incerta (ZI) is a subthalamic nucleus connected to several structures, some of them known to be involved with antinociception. The ZI itself may be involved with both antinociception and nociception. The antinociceptive effects of stimulating the ZI with glutamate using the rat tail-flick test and a rat model of incision pain were examined. The effects of intraperitoneal antagonists of acetylcholine, noradrenaline, serotonin, dopamine, or opioids on glutamate-induced antinociception from the ZI in the tail-flick test were also evaluated. The injection of glutamate (7 ?g/0.25 ?l) into the ZI increased tail-flick latency and inhibited post-incision pain, but did not change the animal performance in a Rota-rod test. The injection of glutamate into sites near the ZI was non effective. The glutamate-induced antinociception from the ZI did not occur in animals with bilateral lesion of the dorsolateral funiculus, or in rats treated intraperitoneally with naloxone (1 and 2 m/kg), methysergide (1 and 2 m/kg) or phenoxybenzamine (2 m/kg), but remained unchanged in rats treated with atropine, mecamylamine, or haloperidol (all given at doses of 1 and 2 m/kg). We conclude that the antinociceptive effect evoked from the ZI is not due to a reduced motor performance, is likely to result from the activation of a pain-inhibitory mechanism that descends to the spinal cord via the dorsolateral funiculus, and involves at least opioid, serotonergic and ?-adrenergic mechanisms. This profile resembles the reported effects of these antagonists on the antinociception caused by stimulating the periaqueductal gray or the pedunculopontine tegmental nucleus. PMID:22327011

Petronilho, Ariane; Reis, Gláucia M; Dias, Quintino M; Fais, Rafael S; Prado, Wiliam A

2012-02-01

91

Contraceptive potential of porcine and feline zona pellucida A, B and C subunits in domestic cats.  

PubMed

Feral cat populations are a major problem in many urban regions throughout the world, threatening biodiversity. Immunocontraception is considered as an alternative and a more humane means to control overpopulation of pest animals than current methods including trapping, poisoning and shooting. In this study, we evaluate porcine zona pellucida (ZP) polypeptide (55 kDa) and feline ZP A, B and C subunits expressed by plasmid vectors as candidate vaccines against fertility in the female domestic cat. Cats were injected subcutaneously with three doses of the ZP vaccines. Vaccinated cats were compared with naïve cats for ZP-antibody response, ovarian histology and fertility after mating. Vaccination with native porcine ZP 55 kDa polypeptide induced anti-porcine ZP antibodies detected by ELISA. However, these antibodies did not cross-react with feline ZP as assessed by immunohistochemistry and no effect on fertility in vivo was observed after mating. However, vaccination of cats with feline ZPA or feline ZPB+C DNA vectors elicited circulating antibodies specific for feline ZP as assessed by ELISA, with reactivity to native feline ZP in ovarian follicles in situ. Vaccination with feline ZPA and ZPB+C DNA did not elicit changes in ovarian histology. Although sample sizes were small, conception rates in mated females were 25 and 20% in the ZPA and ZPB+C vaccinated groups respectively, compared with 83% in the control group. We conclude that feline ZPA and ZPB+C subunits are potential candidate antigens for immunocontraceptive vaccines in the domestic cat. PMID:19279201

Eade, Joyce A; Roberston, Ian D; James, Cassandra M

2009-03-11

92

Angiotensin II regulates adrenal vascular tone through zona glomerulosa cell-derived EETs and DHETs.  

PubMed

Elevated concentrations of aldosterone are associated with several cardiovascular diseases. Angiotensin II (Ang II) increases aldosterone secretion and adrenal blood flow. This concurrent increase in steroidogenesis and adrenal blood flow is not understood. We investigated the role of zona glomerulosa (ZG) cells in the regulation of vascular tone of bovine adrenal cortical arteries by Ang II. ZG cells enhanced endothelium-dependent relaxations to Ang II. The ZG cell-dependent relaxations to Ang II were unchanged by removing the endothelium-dependent response to Ang II. These ZG cell-mediated relaxations were ablated by cytochrome P450 inhibition, epoxyeicosatrienoic acid (EET) antagonism, and potassium channel blockade. Analysis of ZG cell EET production by liquid chromatography/mass spectrometry demonstrated an increase in EETs and dihydroxyeicosatrienoic acids with Ang II stimulation. These EETs and dihydroxyeicosatrienoic acids produced similar concentration-dependent relaxations of adrenal arteries, which were attenuated by EET antagonism. Whole-cell potassium currents of adrenal artery smooth muscle cells were increased by Ang II stimulation in the presence of ZG cells but decreased in the absence of ZG cells. This increase in potassium current was abolished by iberiotoxin. Similarly, 14,15-EET induced concentration-dependent increases in potassium current, which was abolished by iberiotoxin. ZG cell aldosterone release was not directly altered by EETs. These data suggest that Ang II stimulates ZG cells to release EETs and dihydroxyeicosatrienoic acids, resulting in potassium channel activation and relaxation of adrenal arteries. This provides a mechanism by which Ang II concurrently increases adrenal blood flow and steroidogenesis. PMID:21199991

Kopf, Phillip G; Gauthier, Kathryn M; Zhang, David X; Falck, John R; Campbell, William B

2011-01-03

93

Adrenal cortex remodeling and functional zona glomerulosa hyperplasia in primary aldosteronism.  

PubMed

Primary aldosteronism is the most common form of secondary hypertension with hypokalemia and suppressed renin-angiotensin system caused by autonomous aldosterone production. Our aim was to compare zona glomerulosa (ZG) structure and function between control adrenals and the peritumoral tissue from patients operated on for aldosterone-producing adenoma. ZG morphology and CYP11B1, CYP11B2, and disabled 2 expression were studied in 15 control adrenals and 25 adrenals with aldosterone-producing adenoma. A transcriptome analysis was done using publicly available data sets. In control adrenals, ZG was discontinuous, and CYP11B2 expression was focal or partly continuous and localized to 3 structures, foci, megafoci, and aldosterone-producing cell clusters. CYP11B2 expression was restricted to a limited number of ZG cells expressing Dab2 but not CYP11B1; aldosterone-producing cell clusters were composed of cells with an intermediate phenotype expressing CYP11B2 but not disabled 2 or CYP11B1. In peritumoral tissue, large remodeling of the adrenal cortex was observed with increased nodulation and decreased vascularization that were not correlated with CYP11B2 expression. In 17 out of 25 adrenals, hyperplasia of adjacent ZG was observed with persistent expression of CYP11B2 that was extended to the entire ZG. In all of the adrenals from patients with aldosterone-producing adenoma, CYP11B2 expression was present in foci, megafoci, and aldosterone-producing cell clusters. Transcriptome profiling indicates a close relationship between peritumoral and control adrenal cortex. In conclusion, adrenal cortex remodeling, reduced vascularization, and ZG hyperplasia are major features of adrenals with aldosterone-producing adenoma. Transcriptional phenotyping is not in favor of this being an intermediate step toward the formation of aldosterone-producing adenoma. PMID:20937967

Boulkroun, Sheerazed; Samson-Couterie, Benoit; Dzib, José-Felipe Golib; Lefebvre, Hervé; Louiset, Estelle; Amar, Laurence; Plouin, Pierre-François; Lalli, Enzo; Jeunemaitre, Xavier; Benecke, Arndt; Meatchi, Tchao; Zennaro, Maria-Christina

2010-10-11

94

Effects and mechanisms of nonylphenol on corticosterone release in rat zona fasciculata-reticularis cells.  

PubMed

Alkylphenol ethoxylate, consisting of ?80% nonylphenol ethoxylate (NPEO), is a major group of nonionic surfactant. The primary degradation product of NPEO, nonylphenol (NP), interferes with reproduction, induces cell death in gonads, and leads to changes in other reproductive parameters. With such apparent stress, NP is believed to induce stress response mechanism, i.e., adrenal cortical hormone. However, the effects and action mechanisms of NP on rat adrenal zona fasciculata-reticularis (ZFR) cells are still unclear. This study explored the effects of NP on corticosterone release. ZFR cells were incubated with NP in the presence or absence of adrenocorticotropin (ACTH), 8-bromo-cyclic 3',5'-adenosine monophosphate (8-Br-cAMP), forskolin (FSK), 25-hydroxyl cholesterol (25-OH-cholesterol), pregnenolone, progesterone, or deoxycorticosterone at 37°C for 1 h. The concentrations of corticosterone or pregnenolone in the spent media were measured by radioimmunoassay. The expressions of steroidogenic acute regulatory (StAR) protein, cytochrome P450 side-chain cleavage (P450scc) protein, and 11?-hydroxylase in the cells were measured by Western blot. The data demonstrated that (1) NP stimulated corticosterone release induced by ACTH, 8-Br-cAMP, FSK, 25-OH-cholesterol, pregnenolone, progesterone, or deoxycorticosterone; (2) NP significantly increased pregnenolone release in the control, 25-OH-cholesterol, trilostane, and 25-OH-cholesterol + trilostane groups; (3) NP-stimulated corticosterone release was estrogen receptor dependent, but mediated by nitric oxide and p38 mitogen-activated protein kinase pathway independent; and (4) NP did not affect StAR, 11?-hydroxylase, or P450scc protein expression. These results suggest that NP acts directly on rat ZFR cells to stimulate corticosterone release and that the stimulation mechanism of NP mediates through post-cAMP corticosterone manufacture enzymes, i.e., P450scc and 11?-hydroxylase. PMID:20837582

Chang, Ling-Ling; Alfred Wun, Wan-Song; Wang, Paulus S

2010-09-13

95

Políticas del sitio web  

Cancer.gov

En el sitio web del NCI se proporcionan enlaces a otros sitios web con fines informativos y para conveniencia del público. Si el usuario selecciona un sitio web externo, saldrá del sitio web del NCI y estará sujeto a las políticas de privacidad y seguridad de dicho sitio.

96

Two subunits of the 55 K porcine zona pellucida glycoprotein family are immunologically distinct  

SciTech Connect

The 55K glycoprotein family (ZP3) of the porcine zona pellucida is comprised of two subunits of 46 K and 45 K which can be resolved by endo-..beta..-galactosidase digestion of ZP3 followed by reversed phase HPLC on Vydac C4 resin. Gel electrophoresis revealed that the 46 K component (EBDG..cap alpha..) is approx. 95% pure and the 45 K component (EBGD..beta..) is 100% pure. In the present study, these two subunits were evaluated immunologically by RIA. Under similar reaction protocols (chloramine-T iodination procedure) comparable specific activities were obtained for EBGD..cap alpha.. (33.06 +/- 7.5 ..mu..ci/..mu..gm), EBGD..beta.. (30.45 +/- 1.6) and ZP3 (26.3 +/- 1.3). Antibody (Ab) titration studies revealed that EBGD..cap alpha.. and ..beta.. are potent immunogens and /sup 125/I-EBGD..cap alpha.. showed minimal cross reactivity to EBGD..beta..-Ab (8% bound at 1:500 dilution), whereas, /sup 125/I-EBGD..beta.. showed a greater degree of cross reactivity to EBGD..cap alpha..-Ab (23% bound at 1:500 dilution). Maximum binding for the two labeled antigens against homologous Abs (1:500) was > 60%. Dose response studies revealed that in the /sup 125/I-EBGD..cap alpha.. vs EBGD..cap alpha.. -Ab system, the 50% intercept was 3.25 +/- 0.32 ng for EBGD..cap alpha.. and 472.43 +/- 30.26 ng for EBGD..beta.. (p < 0.01), whereas, in the /sup 125/I-EBGD..beta.. vs EBGD..beta..-Ab system the 50% intercept was 3.51 +/- 0.58 for EBGD..beta.. and 166.77 +/- 49.20 for EBGD..cap alpha.. (p < 0.01). No significant differences were observed in the slopes of the dose response curves. It is concluded that the two subunits of ZP3 possess distinct immunologic characteristics as evaluated by RIA.

Subramanian, M.G.; Yurewicz, E.C.; Sacco, A.G.

1986-03-01

97

Imaging for deep brain stimulation: The zona incerta at 7 Tesla  

PubMed Central

AIM: To evaluate different promising magnetic resonance imaging (MRI) methods at 7.0 Tesla (T) for the pre-stereotactic visualization of the zona incerta (ZI). METHODS: Two neuroradiologists qualitatively and quantitatively examined T2-turbo spin-echo (T2-TSE), T1-weighted gradient-echo, as well as FLASH2D-T2Star and susceptibility-weighted imaging (SWI) for the visualization of the ZI at 7.0 T MRI. Delineation and image quality for the ZI were independently examined using a 6-scale grading system. Inter-rater reliability using Cohen’s kappa coefficient (?) were assessed. Contrast-to-noise ratios (CNR), and signal-to-noise ratios (SNR) for the ZI were calculated for all sequences. Differences in delineation, SNR, and CNR between the sequences were statistically assessed using a paired t-test. For the anatomic validation the coronal FLASH2D-T2Star images were co-registered with a stereotactic atlas (Schaltenbrand-Wahren). RESULTS: The rostral part of the ZI (rZI) could easily be identified and was best and reliably visualized in the coronal FLASH2D-T2Star images. The caudal part was not definable in any of the sequences. No major artifacts in the rZI were observed in any of the scans. FLASH2D-T2Star and SWI imaging offered significant higher CNR values for the rZI compared to T2-TSE images (P > 0.05). The co-registration of the coronal FLASH2D-T2Star images with the stereotactic atlas schema (Schaltenbrand-Wahren) confirmed the correct localization of the ZI in all cases. CONCLUSION: FLASH2D-T2Star imaging (particularly coronal view) provides the reliable and currently optimal visualization of the rZI at 7.0 T. These results can facilitate a better and more precise targeting of the caudal part of the ZI than ever before.

Kerl, Hans U; Gerigk, Lars; Brockmann, Marc A; Huck, Sonia; Al-Zghloul, Mansour; Groden, Christoph; Hauser, Thomas; Nagel, Armin M; Nolte, Ingo S

2013-01-01

98

Evaluation of zona pellucida birefringence intensity during in vitro maturation of oocytes from stimulated cycles  

PubMed Central

Background This study evaluated whether there is a relationship between the zona pellucida birefringence (ZP-BF) intensity and the nuclear (NM) and cytoplasmic (CM) in vitro maturation of human oocytes from stimulated cycles. Results The ZP-BF was evaluated under an inverted microscope with a polarizing optical system and was scored as high/positive (when the ZP image presented a uniform and intense birefringence) or low/negative (when the image presented moderate and heterogeneous birefringence). CM was analyzed by evaluating the distribution of cortical granules (CGs) throughout the ooplasm by immunofluorescence staining. CM was classified as: complete, when CG was localized in the periphery; incomplete, when oocytes presented a cluster of CGs in the center; or in transition, when oocytes had both in clusters throughout cytoplasm and distributed in a layer in the cytoplasm periphery Nuclear maturation: From a total of 83 germinal vesicle (GV) stage oocytes, 58 of oocytes (69.9%) reached NM at the metaphase II stage. From these 58 oocytes matured in vitro, the high/positively scoring ZP-BF was presented in 82.7% of oocytes at the GV stage, in 75.8% of oocytes when at the metaphase I, and in 82.7% when oocytes reached MII. No relationship was observed between NM and ZP-BF positive/negative scores (P = 0.55). These variables had a low Pearson's correlation coefficient (r = 0.081). Cytoplasmic maturation: A total of 85 in vitro-matured MII oocytes were fixed for CM evaluation. Forty-nine oocytes of them (57.6%) showed the complete CM, 30 (61.2%) presented a high/positively scoring ZP-BF and 19 (38.8%) had a low/negatively scoring ZP-BF. From 36 oocytes (42.3%) with incomplete CM, 18 (50%) presented a high/positively scoring ZPBF and 18 (50%) had a low/negatively scoring ZP-BF. No relationship was observed between CM and ZP-BF positive/negative scores (P = 0.42). These variables had a low Pearson's correlation coefficient (r = 0.11). Conclusions The current study demonstrated an absence of relationship between ZP-BF high/positive or low/negative score and nuclear and cytoplasmic in vitro maturation of oocytes from stimulation cycles.

2011-01-01

99

Inhibitory effects of digoxin and digitoxin on corticosterone production in rat zona fasciculata-reticularis cells  

PubMed Central

The aim of the present study was to investigate the direct effects and action mechanisms of digitalis on the production of corticosterone in rat adrenocortical cells. Male rats were challenged with digoxin (1 ?g ml?1 kg?1) in the presence or absence of adrenocorticotropin (ACTH, 5 ?g ml?1 kg?1) administered by intravenous injection to the right jugular vein. Blood samples were collected at 0, 30, 60, and 120 min following the challenge. The concentration of corticosterone in the rat plasma samples was measured by radioimmunoassay. Zona fasciculata-reticularis (ZFR) cells in male rats were prepared and then incubated with or without digoxin or digitoxin in the presence or absence of ACTH (10?9 M), forskolin (10?7 M), 8-bromo-cyclic 3? : 5?-adenosine monophosphate (10?4 M), cyclopiazonic acid (CPA, 10?5 M), trilostane (10?6 M), 25-OH-cholesterol (10?5 M), pregnenolone (10?5 M), progesterone (10?5 M), or deoxycorticosterone (10?5 M) at 37°C for 1 h before collection of the media. Corticosterone or pregnenolone levels were measured by radioimmunoassay. A single injection of digoxin did not alter the basal level of plasma corticosterone, but did inhibit the level of plasma corticosterone released in response to ACTH in vivo. Administration of digoxin or digitoxin decreased both spontaneous and ACTH-stimulated release of corticosterone in vitro. Digoxin (10?7–10?5 M) and digitoxin (10?7–10?5 M), but not ouabain (10?7–10?5 M), dose-dependently inhibited corticosterone production in response to forskolin and 8-Br-cyclic AMP in rat ZFR cells. Both digoxin (10?6–10?5 M) and digitoxin (10?6–10?5 M) attenuated corticosterone production in response to CPA. Digoxin (10?5 M) or digitoxin (10?5 M) inhibited cytochrome P450 side-chain cleavage enzyme (cytochrome P450scc) activity (catalyses conversion of cholesterol to pregnenolone in the presence of trilostane) in rat ZFR cells. The enzyme activity of 11 ?-hydroxylase (catalyses conversion of deoxycorticosterone to corticosterone) in ZFR cells was also inhibited by the administration of digoxin (10?5 M) or digitoxin (10?5 M). These results together suggest that digoxin and digitoxin decrease the release of corticosterone by acting directly on ZFR cells via a Na+, K+-ATPase-independent mechanism involving the inhibition of the activities of adenylyl cyclase, cytochrome P450scc and 11 ?-hydroxylase, as well as the functioning of cyclic AMP and intracellular calcium.

Wang, Shyi-Wu; Pu, Hsaio-Fung; Kan, Shu-Fen; Tseng, Chiung-I; Lo, Ming-Jae; Wang, Paulus S

2004-01-01

100

Calcium-free vitrification reduces cryoprotectant-induced zona pellucida hardening and increases fertilization rates in mouse oocytes.  

PubMed

Despite the success of embryo cyropreservation, routine oocyte freezing has proved elusive with only around 200 children born since the first reported birth in 1986. The reason for the poor efficiency is unclear, but evidence of zona pellucida hardening following oocyte freezing indicates that current protocols affect oocyte physiology. Here we report that two cryoprotectants commonly used in vitrification procedures, dimethyl sulfoxide (DMSO) and ethylene glycol, cause a large transient increase in intracellular calcium concentration in mouse metaphase II (MII) oocytes comparable to the initial increase triggered at fertilization. Removal of extracellular calcium from the medium failed to affect the response exacted by DMSO challenge, but significantly reduced the ethylene glycol-induced calcium increase. These results suggest that the source of the DMSO-induced calcium increase is solely from the internal calcium pool, as opposed to ethylene glycol that causes an influx of calcium across the plasma membrane from the external medium. By carrying out vitrification in calcium-free media, it was found that zona hardening is significantly reduced and subsequent fertilization and development to the two-cell stage significantly increased. Furthermore, such calcium-free treatment appears not to affect the embryo adversely, as shown by development rates to the blastocyst stage and cell number/allocation. Since zona hardening is one of the early activation events normally triggered by the sperm-induced calcium increases observed at fertilization, it is possible that other processes are negatively affected by the calcium rise caused by cryoprotectants used during oocyte freezing, which might explain the current poor efficiency of this technique. PMID:16388009

Larman, Mark G; Sheehan, Courtney B; Gardner, David K

2006-01-01

101

Long term effects of the hypocholesterolaemic drug 4-aminopyrazolo-pyrimidine on the zona fasciculata of the rat adrenal cortex.  

PubMed Central

Prolonged 4-APP administration to dexamethasone/ACTH-treated rats was found to lower both plasma and intra-adrenal gland cholesterol concentrations. Morphological examination showed that zona fasciculata cells underwent a severe lipid droplet depletion, and a conspicuous increase of agranular endoplasmic reticulum and peroxisomes. The blood level of corticosterone remained unchanged. A high cholesterol diet completely reversed the effects of 4-APP treatment. The hypothesis is discussed that these morphological changes are the expression of a compensatory response enabling rat adrenocortical cells to maintain an adequate level of hormone output even in the absence of a normal supply of exogenous cholesterol. Images Figs. 1-2 Figs. 3-4

Mazzocchi, G; Robba, C; Meneghelli, V; Nussdorfer, G G

1986-01-01

102

Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis  

Microsoft Academic Search

Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNAs were\\u000a synthesized from the ovary of A. sinensis, and the full-length cDNAs of three zona pellucida glycoprotein 3 genes (the new gene named AsZP3) were cloned and sequenced. AsZP3.1, AsZP3.2, and AsZP3.3 were 1,388 base pairs (bp), 1,288,

Li Chuang-Ju; Wei Qi-Wei; Chen Xi-Hua; Zhou Li; Cao Hong; Gan Fang; Gui Jian-Fang

2011-01-01

103

The effects of potassium, 5-hydroxytryptamine, adrenocorticotrophin and angiotensin II on the concentration of adenosine 3?:5?-cyclic monophosphate in suspensions of dispersed rat adrenal zona glomerulosa and zona fasciculata cells  

PubMed Central

Dispersed rat adrenal cells prepared from both the capsule and the decapsulated gland were used to investigate the effects on cyclic AMP accumulation of known stimuli of steroidogenesis [ACTH (adrenocorticotrophin), angiotensin II, K+ ions and 5-hydroxytryptamine]. Since glomerulosa-cell preparations from capsular strippings are normally contaminated with a proportion of fasciculata cells, cells purified by fractionation on a bovine serum albumin gradient were also used. The results showed that: (1) ACTH and angiotensin II stimulated cyclic AMP accumulation in both fractionated and unfractionated zona fasciculata cells; (2) 5-hydroxytryptamine and an increased extracellular K+ concentration (from 3.6 to 8.4mm) had no effect on cyclic AMP concentrations in fasciculata cell preparations; (3) the addition of ACTH, angiotensin II, 5-hydroxytryptamine or K+ to the incubation medium resulted in increased cyclic AMP concentrations in unpurified zona glomerulosa cell preparations; (4) fractionation and hence the virtual elimination of fasciculata contamination, did not affect the response to 5-hydroxytryptamine and increased K+ concentration. However, the responses to ACTH and angiotensin II were markedly lowered but not abolished. These results strongly suggest a link between cyclic AMP production and steroidogenesis in the zone of the adrenal gland that specifically secretes aldosterone. All four agents used stimulated both steroid output and cyclic AMP accumulation. However, at certain doses of 5-hydroxytryptamine, K+ and angiotensin II the significant increases in corticosterone output were not accompanied by measurable increases in cyclic AMP accumulation.

Albano, Janet D. M.; Brown, Barry L.; Ekins, Roger P.; Tait, Sylvia A. S.; Tait, James F.

1974-01-01

104

Application of laser-assisted zona drilling to in vitro fertilization of cryopreserved mouse oocytes with spermatozoa from a subfertile transgenic mouse.  

PubMed

Development of assisted reproductive technologies is necessary to obtain fertilized oocytes in a subfertile transgenic mouse strain. Here, we showed the application of laser-assisted drilling of the zona pellucida to in vitro fertilization of cryopreserved mouse oocytes with sperm from subfertile transgenic mice (C57BL/6N-Tg(UCP/FAD2)U8 strain). After cryopreservation by vitrification, the recovery and survival rates of the zona-drilled mouse oocytes were 97% (97/100) and 94% (91/97), respectively. In vitro fertilization of the cryopreserved zona-drilled mouse oocytes with sperm from the subfertile transgenic mice was greatly facilitated (60%, 55/91) compared to that of the cryopreserved zona-intact mouse oocytes (11%, 81/768). In vitro fertilized embryos that developed to the 2-cell stage were again cryopreserved by vitrification, and after warming they were transferred into recipient females. Subsequently, six viable offspring were delivered, and all were confirmed to be transgenic mice. These results indicate that laser-assisted zona drilling of oocytes combined with cryopreservation by vitrification may be a useful approach for large-scale production of in vitro fertilized embryos for managing transgenic mouse strains with reproductive disabilities such as subfertile sperm. PMID:16807506

Anzai, Masayuki; Nishiwaki, Megumi; Yanagi, Miho; Nakashima, Tatsuyuki; Kaneko, Takehito; Taguchi, Yoshitomo; Tokoro, Mikiko; Shin, Seung-Wook; Mitani, Tasuku; Kato, Hiromi; Matsumoto, Kazuya; Nakagata, Naomi; Iritani, Akira

2006-06-28

105

La virtualización del cuerpo a través del \\  

Microsoft Academic Search

Hablar de un cuerpo virtualizado es hablar de su problematización y consecuente actualización, una de las principales consecuencias de este proceso de transfiguración del cuerpo estaría marcada por una adjudicación actualizada de un cambio. El cuerpo es simultáneamente un cuerpo-materia prima, modelo físico para la conversión óptico-iconográfica digital, y un cuerpo interfaz, que permite el acceso a la morfogénesis del

Eduardo Castro Pinzón; José Luis Troncoso

2005-01-01

106

Dinámica del Desempeño Académico  

Microsoft Academic Search

Este trabajo se ocupa del progreso, luego del ingreso, de los estudiantes de la cohorte 2000 de la Facultad de Ciencias Económicas de la Universidad Nacional de La Plata. Si bien hay antecedentes de medición del rendimiento de los estudiantes de la Facultad y sus factores explicativos, se trata de estudios realizados en base a encuestas a alumnos regulares en

Luciano Di Gresia; Alberto Porto

2004-01-01

107

Efficiency of asynchronously in vitro-matured oocytes as recipients for nuclear transfer and of blind enucleation in zona-free bovine cloning.  

PubMed

We studied the dynamics of in vitro maturation of bovine oocytes, the efficiency of asynchronously matured oocytes as recipients for the generation of embryos produced by nuclear transfer, and the potential for using blind enucleation of zona-free bovine oocytes in bovine cloning. At 15 h after the initiation of maturation (hpm), oocytes were freed from both cumulus cells and the zona pellucida, and the dynamics of oocyte maturation were monitored every 30 min through the criterion of extrusion of the first polar body (PB1). More than 41% of bovine oocytes had extruded PB1 by 16.5 hpm, and were designated as representing a group of rapidly maturing oocytes. A second group, comprising about 25% of all oocytes, had extruded PB1 by 18.5-20.0 hpm. Examination of Hoechst 33342-stained samples demonstrated that PB1 on the surfaces of zona-free bovine oocytes were always located near the maternal chromosomes. Zona-free oocytes were enucleated by removing PB1 and about 3% of the adjacent oocyte cytoplasm without chromatin staining. Successful enucleation of zona-free bovine oocytes was achieved in 96.9% of cases. The rate of development to the blastocyst stage was significantly greater in embryos reconstructed from rapidly maturing oocytes (47.8%) than with oocytes maturing at 18.0-20.0 hpm (33.3%). Overall, two large groups of bovine oocytes could be distinguished during in vitro maturation by the time required to reach the second stage of metaphase. Bovine embryos reconstructed from rapidly maturing enucleated oocytes had a significantly greater rate of development to the blastocyst stage than did embryos derived from later-maturing oocytes. We conclude that blind enucleation is a simple and efficient method for preparing cytoplasts in zona-free bovine cloning. PMID:19508117

Malenko, Galina P; Stepanov, Oleg I; Komissarov, Andrey V; Antipova, Tatyana A; Pinyugina, Marina V; Prokofiev, Michael I

2009-06-01

108

Zona pellucida birefringence correlates with developmental capacity of bovine oocytes classified by maturational environment, COC morphology and G6PDH activity.  

PubMed

In the present study we aimed to analyse structural changes during in vitro maturation of the bovine zona pellucida (ZP) by scanning electron microscopy (SEM) ands zona pellucida birefringence (ZPB). Here we show that alterations during in vitro maturation invasively analysed by SEM are reflected in ZPB. In vivo-matured oocytes displayed significantly lower birefringence parameters and significantly higher blastocyst rates compared with in vitro-derived oocytes (39.1% vs 21.6%). The same was observed for in vitro-matured oocytes with cumulus-oocyte complex (COC) Quality 1 (Q1) compared with Q3-COCs with respect to zona birefringence and developmental capacity. Immature oocytes with Q1-COCs displayed higher ZPB values and a higher developmental capacity to the blastocyst stage (27.7% vs 16.9%) compared with immature Q3-COCs. Considering in vitro-matured oocytes, only those with Q1-COC showed a trend for ZPB similar to in vivo-matured oocytes. Therefore, a decreasing trend for ZPB during in vitro maturation seems to be typical for high-quality oocytes and successful cytoplasmic maturation. In accordance, fully-grown immature oocytes reached significantly higher blastocyst rates (32.0% vs 11.5%) and lower ZPB values compared with still-growing ones. In conclusion, we successfully evaluated the applicability of zona imaging to bovine oocytes: alterations during in vitro maturation invasively analysed by scanning electron microscopy were reflected in the birefringence of the zona pellucida of bovine oocytes affecting developmental capacity at the same value. Therefore ZPB measurement by live zona imaging has potential to become a new tool to assess correctness of in vitro maturation and to predict developmental competence. PMID:22541545

Held, Eva; Mertens, Eva-Maria; Mohammadi-Sangcheshmeh, Abdollah; Salilew-Wondim, Dessie; Besenfelder, Urban; Havlicek, Vitezslav; Herrler, Andreas; Tesfaye, Dawit; Schellander, Karl; Hölker, Michael

2012-01-01

109

Productividad primaria del fitoplancton en la zona eufótica del Sistema de la Corriente de California estimada mediante imágenes del CZCS Phytoplankton primary productivity in the euphotic zone of the California Current System estimated from CZCS imagery  

Microsoft Academic Search

We estimated phytoplankton primary productivity (PP) in the euphotic zone of the California Current System using chlorophyll concentration (Chl) from CZCS imagery. The area was divided into two regions, Southern California (SC) and Baja California (BC), with inshore and offshore subregions, and into two seasons, cool and warm. The Chl and PP values were in general higher and more variable

OA Barocio-León; R Millán-Núñez; E Santamaría-del-Ángel; A González-Silvera

110

Moléculas orgánicas obtenidas en simulaciones experimentales del medio interestelar.  

NASA Astrophysics Data System (ADS)

Las nubes moleculares son regiones de formación de estrellas, con temperaturas cinéticas entre 10-50 K y densidades de 103-106 átomos cm-3. Su materia está formada por gas y polvo interestelar. Estas partículas de polvo están cubiertas por una fina capa de hielo, de unos 0.01 ?m, que contiene H2O y a menudo CO, CO2, CH3OH y NH3. El hielo es presumiblemente irradiado por fotones ultravioleta y rayos cósmicos en las zonas poco profundas de las nubes moleculares y las regiones circunestelares. En un sistema de vacío, P ˜ 10-7 mbar, simulamos la deposición de hielo a partir de 10 K y la irradiación ultravioleta por medio de una lámpara de descarga de hidrógeno activada con microondas. La evolución del hielo se observa por medio de un espectrómetro infrarrojo. De este modo es posible determinar la composición del hielo observado en el medio interestelar y predecir la presencia de moléculas aún no detectadas en el espacio, que han sido producto del procesamiento del hielo en nuestros experimentos. También es posible calentar el sistema hasta temperatura ambiente para sublimar el hielo depositado. Cuando el hielo ha sido previamente irradiado, se observa un residuo compuesto por moléculas orgánicas complejas, algunas prebióticas, como varios ácidos carboxílicos, aminas, amidas, ésteres y en menor proporción moléculas heterocíclicas y aminoácidos. Algunas de estas moléculas podrían detectarse en estado gaseoso por medio de observaciones milimétricas y de radio. También podrían estar presentes en el polvo cometario, cuyo análisis químico está planeado por las misiones Stardust y Rosetta. Mientras tanto, nuestro grupo está llevando a cabo el análisis de partículas de polvo interplanetario (IDPs), algunas de las cuales pueden ser de origen cometario. Al igual que ocurre con los productos obtenidos por irradiación del hielo en nuestros experimentos, algunas IDPs son ricas en material orgánico que contiene oxígeno.

Muñoz-Caro, Guillermo Manuel

111

The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa: differences from the zona pellucida  

PubMed Central

The acrosome reaction (AR), an absolute requirement for spermatozoa and egg fusion, requires the influx of Ca2+ into the spermatozoa through voltage-dependent Ca2+ channels and store-operated channels. Maitotoxin (MTx), a Ca2+-mobilizing agent, has been shown to be a potent inducer of the mouse sperm AR, with a pharmacology similar to that of the zona pellucida (ZP), possibly suggesting a common pathway for both inducers. Using recombinant human ZP3 (rhZP3), mouse ZP and two MTx channel blockers (U73122 and U73343), we investigated and compared the MTx- and ZP-induced ARs in human and mouse spermatozoa. Herein, we report that MTx induced AR and elevated intracellular Ca2+ ([Ca2+]i) in human spermatozoa, both of which were blocked by U73122 and U73343. These two compounds also inhibited the MTx-induced AR in mouse spermatozoa. In disagreement with our previous proposal, the AR triggered by rhZP3 or mouse ZP was not blocked by U73343, indicating that in human and mouse spermatozoa, the AR induction by the physiological ligands or by MTx occurred through distinct pathways. U73122, but not U73343 (inactive analogue), can block phospholipase C (PLC). Another PLC inhibitor, edelfosine, also blocked the rhZP3- and ZP-induced ARs. These findings confirmed the participation of a PLC-dependent signalling pathway in human and mouse zona protein-induced AR. Notably, edelfosine also inhibited the MTx-induced mouse sperm AR but not that of the human, suggesting that toxin-induced AR is PLC-dependent in mice and PLC-independent in humans.

Chavez, Julio C; de Blas, Gerardo A; de la Vega-Beltran, Jose L; Nishigaki, Takuya; Chirinos, Mayel; Gonzalez-Gonzalez, Maria Elena; Larrea, Fernando; Solis, Alejandra; Darszon, Alberto; Trevino, Claudia L

2011-01-01

112

ESTUDIO DE LA FERTILIDAD DE SUELOS DEDICADOS A POTREROS EN LA ZONA NORTE DE HEREDIA, MEDIANTE LA TECNICA DEL ELEMENTO FALTANTE.l  

Microsoft Academic Search

Fertility of grassland soils from north Heredia estimated by the missing element technique. An attempt was made to characterize the nutritional status of fours grassland soils from the slopes of the Poas-Barva volcanic area, using the missing element technique in a greenhouse study. In general these soils gave the highest reponse to P, N. Mn, K, Ca, and Cu in

Rafael Salas; Rolando Pacheco

113

Talla de primera madurez sexual y fecundidad parcial de la merluza común (Merluccius gayi, Guichenot 1848) del área de la pesquería industrial de la zona de Talcahuano, Chile  

Microsoft Academic Search

Length at maturity and batch fecundity of the hake south pacific was estimated for the time period extending from April to June of 1992. The samples were collected on board industrial trawlers operating in the Chilean fishery with base port in Talcahuano, in an area, which extends from 34°20' S to 38° S. For the length at first maturity estimations

José F. Cerna; Ciro Oyarzún

1998-01-01

114

Tyrosine phosphorylation on capacitated human sperm tail detected by immunofluorescence correlates strongly with sperm-zona pellucida (ZP) binding but not with the ZP-induced acrosome reaction  

Microsoft Academic Search

BACKGROUND: Protein tyrosine phosphorylation (TP) of human sperm is related to sperm capacitation and zona pellucida (ZP) binding. The aim of this study was to determine whether the TP of capacitated sperm is a useful marker for the ability of sperm to bind to the ZP and undergo the ZP-induced acrosome reaction (AR). METHODS: Semen samples were obtained from 115

D. Y. Liu; G. N. Clarke; H. W. G. Baker

2005-01-01

115

Application of a zona pellucida binding assay (ZBA) in the domestic cat benefits from the use of in vitro matured oocytes  

PubMed Central

Background Zona pellucida binding assays (ZBAs) have proven useful in determining the fertilising ability of spermatozoa in several species. Most ZBAs use fresh or salt-stored oocytes collected from fresh ovaries but because ovaries are not easy to obtain on a regular basis, chilled and frozen-thawed ovaries have been tested, with varying results. The present study tested the hypothesis that cat spermatozoa, either fresh or frozen-thawed, can bind to homologous zona pellucida of oocytes retrieved from frozen-thawed queen ovaries to a similar extent as they can bind to the zona pellucida of fresh, in vitro matured oocytes. Methods Ovaries were collected from queens after routine ovario-hysterectomy and either stored in NaCl at -20°C until use (treatment animals), or used fresh (controls). Cumulus-oocyte complexes (COCs) were retrieved by ovarian slicing from either source and used directly (immature oocytes from frozen-thawed ovaries; treatment animals) or after in vitro maturation (IVM) (fresh ovaries; controls) for 24 hours in TCM 199, supplemented with 1 IU hCG/mL and 0.5 IU eCG/mL and 0.5% bovine serum albumin (BSA). The oocytes were incubated for 4 hours in 5% CO2 in air at 38°C and 100% humidity in the presence of 5 × 106 fresh or frozen-thawed spermatozoa/mL. Representative samples of oocytes were processed for scanning electron microscopy (SEM). Results Both fresh and frozen-thawed spermatozoa bound to the in vitro matured zona pellucida but significantly fewer, or no, spermatozoa bound to frozen-thawed, immature zona pellucida (P < 0.001). Also, more fresh spermatozoa than frozen-thawed spermatozoa bound to the zona pellucida (P < 0.001). The zona pellucida surface differed in morphology (SEM), with in vitro matured oocytes showing a dense surface with few fenestrations in contrast to their frozen-thawed, immature counterparts, where fenestrations were conspicuously larger. Conclusion In conclusion, under the conditions of the present study, immature oocytes recovered from ovaries frozen immersed in NaCl at -20°C are less suitable for use in feline ZBA.

Hermansson, Ulrika; Axner, Eva; Holst, Bodil Strom

2007-01-01

116

Deep Brain Stimulation of Caudal Zona Incerta and Subthalamic Nucleus in Patients with Parkinson's Disease: Effects on Voice Intensity  

PubMed Central

Deep brain stimulation of the subthalamic nucleus (STN-DBS) in patients with Parkinson's disease (PD) affects speech inconsistently. Recently, stimulation of the caudal zona incerta (cZi-DBS) has shown superior motor outcomes for PD patients, but effects on speech have not been systematically investigated. The aim of this study was to compare the effects of cZi-DBS and STN-DBS on voice intensity in PD patients. Mean intensity during reading and intensity decay during rapid syllable repetition were measured for STN-DBS and cZi-DBS patients (eight patients per group), before- and 12 months after-surgery on- and off-stimulation. For mean intensity, there were small significant differences on- versus off-stimulation in each group: 74.2 (2.0)?dB contra 72.1 (2.2)?dB (P = .002) for STN-DBS, and 71.6 (4.1)?dB contra 72.8 (3.4)?dB (P = .03) for cZi-DBS, with significant interaction (P < .001). Intensity decay showed no significant changes. The subtle differences found for mean intensity suggest that STN-DBS and cZi-DBS may influence voice intensity differently.

Lundgren, Sofie; Saeys, Thomas; Karlsson, Fredrik; Olofsson, Katarina; Blomstedt, Patric; Linder, Jan; Nordh, Erik; Zafar, Hamayun; van Doorn, Jan

2011-01-01

117

Deep Brain Stimulation of Caudal Zona Incerta and Subthalamic Nucleus in Patients with Parkinson's Disease: Effects on Diadochokinetic Rate  

PubMed Central

The hypokinetic dysarthria observed in Parkinson's disease (PD) affects the range, speed, and accuracy of articulatory gestures in patients, reducing the perceived quality of speech acoustic output in continuous speech. Deep brain stimulation (DBS) of the subthalamic nucleus (STN-DBS) and of the caudal zona incerta (cZi-DBS) are current surgical treatment options for PD. This study aimed at investigating the outcome of STN-DBS (7 patients) and cZi-DBS (7 patients) in two articulatory diadochokinesis tasks (AMR and SMR) using measurements of articulation rate and quality of the plosive consonants (using the percent measurable VOT metric). The results indicate that patients receiving STN-DBS increased in articulation rate in the Stim-ON condition in the AMR task only, with no effect on production quality. Patients receiving cZi-DBS decreased in articulation rate in the Stim-ON condition and further showed a reduction in production quality. The data therefore suggest that cZi-DBS is more detrimental for extended articulatory movements than STN-DBS.

Karlsson, Fredrik; Unger, Elin; Wahlgren, Sofia; Blomstedt, Patric; Linder, Jan; Nordh, Erik; Zafar, Hamayun; van Doorn, Jan

2011-01-01

118

Effect of the size of zona pellucida opening on hatching in the common marmoset monkey (Callithrix jacchus) embryo.  

PubMed

The use of the common marmoset monkey in biomedical research has increased recently, and further attention has been devoted to this model after the successful production of transgenic marmosets. To extend genetic engineering approaches to widespread biomedical research fields, efficient prolonged in vitro culturing of embryo development is necessary. We aimed to evaluate the effects of the size of the zona pellucida opening on promoting the hatching process in the marmoset embryo. Piezo-microdrilling of a 6-?m opening in eight embryos resulted in four partially hatched embryos and one hatched embryo after 5 days of culture. Piezo-microdrilling a 20-?m opening in 11 embryos resulted in nine partial hatchings and no hatched embryos. Piezo-scraping an 80-?m opening in six embryos resulted in no partially hatched embryos and five hatched embryos. These results suggest that an 80-?m opening, rather than 6-?m or 20-?m openings, is suitable to complete the hatching process in the marmoset embryo. PMID:24000827

Ishibashi, Hidetoshi; Motohashi, Hideyuki H; Kumon, Mami; Yamamoto, Kazuhiro; Okada, Hironori; Okada, Takashi; Seki, Kazuhiko

2013-09-04

119

Effects of components of semen extenders on the binding of stallion spermatozoa to bovine or equine zonae pellucidae.  

PubMed

The effects of semen extender components on the ability of stallion sperm to bind to the zona pellucida (ZP) and the suitability of using bovine ZP for a ZP-binding assay for stallion sperm were investigated in a series of experiments. In Experiment I, binding of stallion sperm to both bovine and equine ZP was significantly increased when a skim milk-based extender (EZM) was used. In Experiment II, a threefold increase in sperm binding to ZP was observed when sperm were diluted in EZM compared with diluents, which contained no milk (TALP, LAC, and EmCare). In Experiment III, centrifuging the sperm through Percoll did not increase sperm binding to the ZP but did remove any positive effect of EZM on sperm-ZP binding. In Experiment IV, exposure of either sperm or ZP to EZM before co-incubation did not increase sperm binding to ZP. In Experiment V, sperm diluted in TALP containing skim milk, EZM, or INRA96 bound more efficiently to the ZP than sperm diluted in TALP without milk proteins. In Experiment VI, sodium caseinate, native phosphocaseinate, and caseinoglycopeptide increased sperm binding to the ZP. In conclusion, diluents containing milk or milk proteins markedly enhanced the number of sperm bound to both equine and bovine ZP. PMID:22419829

Coutinho da Silva, Marco A; Seidel, George E; Squires, Edward L; Graham, James K; Carnevale, Elaine M

2012-03-14

120

Pertussis toxin treatment does not block inhibition by atrial natriuretic factor of aldosterone secretion in cultured bovine zona glomerulosa cells  

SciTech Connect

The authors have previously reported that atrial natriuretic factor (ANF) potently inhibits PGE or forskolin-stimulation aldosterone secretion in bovine zona glomerulosa (ZG) by acting through specific high affinity receptors. In order to evaluate the functional role of the regulatory protein N/sub i/ and the inhibition of adenylate cyclase activity (AC) in ZG, the authors have studied the effect of treatment with PT on inhibition by ANF of aldosterone production. Primary cultures of ZG were treated for 18 hours in serum-free F12 medium with (0-100 ng/ml PT). No effect of PT pretreatment was observed either on basal, PGE-stimulated or ANF-inhibited levels of steroidogenesis. When membranes prepared from control ZG were ADP-ribosylated with (/sup 32/P) NAD in the presence of PT, two toxin-specific bands with 39 Kd and 41 Kd were documented on SDS gel. Cell pretreatment with as low as 1 ng/ml drastically reduced further labelling of these two bands while higher doses completely abolished them. Since PT treatment covalently modifies completely the toxin substrate without altering ANF inhibition of adrenal steroidogenesis, the authors conclude that N/sub i/ is not involved in the mode of action of ANF on aldosterone production.

De Lean, A.; Cantin, M.

1986-03-05

121

Boletín del Instituto Nacional del Cáncer - 07-20-2010  

Cancer.gov

El doctor Harold Varmus, acompañado de su esposa Constance Casey (centro), presta juramento como director del NCI ante la Secretaria del Departamento de Salud y Servicios Humanos, Kathleen Sebelius, el lunes 12 de julio. (Foto cortesía de Chris Smith) El 12 de julio, el doctor Harold Varmus prestó juramento ante la Secretaria del Departamento de Salud y Servicios Humanos de los Estados Unidos, Kathleen Sebelios, como el decimocuarto director del Instituto Nacional del Cáncer.

122

Variations in the gene expression of zona pellucida proteins, zpb and zpc, in female European eel (Anguilla anguilla) during induced sexual maturation.  

PubMed

Vertebrate eggs are surrounded by an extracellular glycoprotein coat termed zona pellucida (ZP). Integrity of ZP is critical for a correct embryo development. Two zona pellucida protein genes (zpb and zpc) from European eel were characterized, specific qPCR assays developed and their expression in immature males and females carried out. An experimental group of silver-stage eel females was maintained at 18 °C and hormonally induced to sexual maturation by weekly injections of carp pituitary extract during 12 weeks. Changes in zpb and zpc expression during sexual maturation were studied in liver and ovary by qPCR. In liver, no changes were recorded during hormonal treatment, while in ovary expression of both genes decreased during sexual development. These results are a first step in the characterization of ZP in European eel and in the understanding of the mechanism underlying egg envelope formation. PMID:22750510

Mazzeo, I; Peñaranda, D S; Gallego, V; Hildahl, J; Nourizadeh-Lillabadi, R; Asturiano, J F; Pérez, L; Weltzien, F-A

2012-06-27

123

El pronóstico del cáncer  

Cancer.gov

Hoja informativa sobre la predicción de resultados y recuperación de una enfermedad, y sobre cómo las estadísticas ayudan a los médicos a hacer una estimación del pronóstico de un paciente con cáncer.

124

Identification of the true human orthologue of the mouse Zp1 gene: evidence for greater complexity in the mammalian zona pellucida?  

Microsoft Academic Search

The mammalian zona pellucida is a mixture of glycoproteins, believed to be encoded by three distinct genes, ZP1\\/ZPB, ZP2\\/ZPA, and ZP3\\/ZPC. We have now determined that the true human orthologue of the mouse Zp1 gene is not ZPB, but that there is a distinct human ZP1 gene. Comparison of the human ZP1 and murine Zp1 genes indicates significant conservation of

David C. Hughes; Christopher L. R. Barratt

1999-01-01

125

Plasma levels of vitellogenin and eggshell zona radiata proteins in 4-nonylphenol and o,p?DDT treated juvenile Atlantic salmon ( Salmo salar)  

Microsoft Academic Search

Induction of vitellogenin (Vtg) in males and juveniles of oviparous vertebrates has been used as a biomarker for xenoestrogens. Recently, we have demonstrated that synthesis of eggshell zona radiata proteins (Zrp) or zonagenesis is an integral aspect of fish oogenesis (Oppen-Berntsen et al. (1994) Journal of Experimental Zoology268, 59–70), and that Zrp synthesis is a sensitive biomarker for nonylphenol (Arukwe

A. Arukwe; T. Celius; B. T. Walther; A. Goksøyr

1998-01-01

126

Two Types of Pharmacologically Distinct Ca 2+ Currents with Voltage-dependent Similarities in Zona Fasciculata Cells Isolated from Bovine Adrenal Gland  

Microsoft Academic Search

.   Voltage-activated Ca2+ currents, in zona fasciculata cells isolated from calf adrenal gland, were characterized using perforated patch-clamp recording.\\u000a In control solution (Ca2+: 2.5 mm) a transient inward current was followed, in 40% of the cells, by a sustained one. In 20 mm Ba2+, 61% of the cells displayed an inward current, which consisted of transient and sustained components. The

A. Guyot; S. Dupré-Aucouturier; C. Ojeda; O. Rougier; A. Bilbaut

2000-01-01

127

Quantification of rainbow trout ( Oncorhynchus mykiss) zona radiata and vitellogenin mRNA levels using real-time PCR after in vivo treatment with estradiol-17? or ?-zearalenol  

Microsoft Academic Search

Estrogen receptor-mediated induction of zona radiata (ZR) and vitellogenin (VTG) mRNA and protein in rainbow trout (Oncorhynchus mykiss) was compared to assess their utility as biomarkers for exposure to estrogenic compounds. Partial sequences of rainbow trout ZR and ?-actin were cloned by reverse transcriptase polymerase chain reaction (RT-PCR) using degenerate primers based on conserved regions across a number of species.

Trine Celius; Jason B. Matthews; John P. Giesy; Timothy R. Zacharewski

2000-01-01

128

Il problema del litio.  

NASA Astrophysics Data System (ADS)

Contents: 1. Introduzione. 2. La nucleosintesi del Big Bang. 3. Il litio nelle stelle di popolazione II. 4. I modelli stellari standard. 5. Il litio negli ammassi aperti. 6. Meccanismi di distruzione "non standard". 7. I modelli non-standard applicati alla popolazione II. 8. L'evoluzione Galattica del litio. 9. Quali stelle producono litio? 10. Il litio come elemento chiave per dare un nome agli oggetti stellari più minuscoli. 11. Conclusioni.

D'Antona, F.

1995-03-01

129

Fisiopatología del estreñimiento  

Microsoft Academic Search

ResumenEl estreñimiento es un trastorno extremadamente frecuente en la infancia, responsable de hasta el 25% de todas las consultas gastroenterológicas pediátricas y el 3% de todas las consultas pediátricas ambulatorias. En el 90% de los casos el origen del trastorno es funcional y sólo en un 10% existe un problema orgánico subyacente. Entre las causas orgánicas corrientes del estreñimiento infantil

Peter J. Milla

2007-01-01

130

Expression of a P-selectin Ligand in Zona Pellucida of Porcine Oocytes and P-selectin on Acrosomal Membrane of Porcine Sperm Cells. Potential Implications for Their Involvement in Sperm-Egg Interactions  

Microsoft Academic Search

The selectin family of cell adhesion mole- cules mediates initial leukocyte adhesion to vascular endothelial cells at sites of inflammation. O -glycan structural similarities between oligosaccharides from human leukocyte P-selectin glycoprotein ligand-1 (PSGL-1) and from zona pellucida glycoproteins of porcine oocytes indicate the possible existence of a P-selectin ligand in the zona pellucida. Here, using bio- chemical as well as

Jian-Guo Geng; Thomas J. Raub; Carolyn A. Baker; Geri A. Sawada; Li Ma; Åke P. Elhammer

1997-01-01

131

Molecular characterization and expression pattern of three zona pellucida 3 genes in the Chinese sturgeon, Acipenser sinensis.  

PubMed

Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNAs were synthesized from the ovary of A. sinensis, and the full-length cDNAs of three zona pellucida glycoprotein 3 genes (the new gene named AsZP3) were cloned and sequenced. AsZP3.1, AsZP3.2, and AsZP3.3 were 1,388 base pairs (bp), 1,288, and 1,290 bp in length, respectively, and they could be translated into proteins with 440, 394, and 398 amino acids, respectively. High level of amino acids sequence identity was seen between AsZP3.2 and AsZP3.3 (about 82%), but they share low identity with AsZP3.1 (26 and 23%, respectively). The AsZP3.1 has 42-50% amino acids sequence identity values with other fish and lower values with higher vertebrates (38%); AsZP3.2 and AsZP3.3 shared about 30-44% sequence identity with higher vertebrates and other fish. RT-PCR analysis indicated that AsZP3.1 displayed a wide tissue distribution at the mRNA levels including liver, kidney, spleen, heart, and ovary, but AsZP3.2 and AsZP3.3 mRNAs were expressed exclusively in the gonad. All three AsZP3 mRNAs were not detected during embryogenesis and early larval development; furthermore, they were not detected in the gonads of 1- and 2-year-old Chinese sturgeons. All three AsZP3 mRNAs were detected in the testes of 3-year-old males and in the ovaries of 4- and 5-year-old female Chinese sturgeons. PMID:21072685

Chuang-Ju, Li; Qi-Wei, Wei; Xi-Hua, Chen; Li, Zhou; Hong, Cao; Fang, Gan; Jian-Fang, Gui

2010-11-12

132

Plasticity of the zona reticularis in the adult marmoset adrenal cortex: voyages of discovery in the New World.  

PubMed

Adrenarche in humans occurs at the age of 5-7 years, yet the process by which dehydroepiandrosterone (DHEA) biosynthesis in the adrenal zona reticularis (ZR) increases so dramatically remains as a matter of debate. One suggestion is that increased DHEA production by P450c17 (CYP17A1 as listed in HUGO Database) in the ZR results from a coincident fall in the expression of HSD3B, which would otherwise compete for pregnenolone substrate. Nonetheless, studies of human and rhesus adrenal show that cytochrome b5 (CYTB5) expression increases in the ZR with DHEA biosynthesis, and cloned human and rhesus P450c17 show selective increases in 17,20-lyase activity in the presence of CYTB5. The marmoset, a New World primate, expresses a fetal zone during development which regresses after birth. Adult males, however, do not develop an obvious functional ZR, while females develop a ZR in a manner that depends on their social/gonadal status. In all social and physiologic states, changes in marmoset ZR function relate directly to changes in the expression of CYTB5. Recent cloning and expression of marmoset P450c17 also show that while amino acid sequence homology is in the order of approximately 85% of that found in human and rhesus sequences, and basal lyase activity is low compared with rhesus, all previously described amino acids critical to human 17,20-lyase activity are completely conserved. Furthermore, the 17,20-lyase activity of the marmoset P450c17 clone is dramatically increased by addition of CYTB5. We propose that these combined data from the marmoset model provide further compelling evidence that the control of ZR CYTB5 expression is a key determinant of ZR function. PMID:19474057

Pattison, J Christina; Abbott, David H; Saltzman, Wendy; Conley, Alan J; Bird, Ian M

2009-05-27

133

Zona Land: Waves  

NSDL National Science Digital Library

This item is a tutorial on the fundamentals of waves. Topics include wave properties and phenomena, types of waves, wave interference, and more. The section on interference includes simple animations of constructive and destructive interference, which can be played in slow motion to allow users to visualize the phenomena before attempting more difficult wave simulations.

Zobel, Edward

2009-03-22

134

Dopamine acts on D2 receptors to increase potassium conductance in neurones of the rat substantia nigra zona compacta.  

PubMed Central

1. Intracellular recordings were made from neurones in the substantia nigra zona compacta in slices of rat mesencephalon in vitro. The majority of neurones fired action potentials spontaneously at 0.2-5.6 Hz. Dopamine, applied either by superfusion or from the tip of a pressurized pipette, prevented spontaneous action potential firing and hyperpolarized the membrane. 2. When the membrane potential was held negative to the threshold for action potential firing, the hyperpolarization evoked by dopamine was accompanied by a fall in input resistance. Under voltage clamp, dopamine produced an outward membrane current associated with an increase in membrane conductance. The effects of superfused dopamine on firing rate, membrane potential and membrane current were concentration dependent in the range 1-100 microM. 3. The reversal potential for the hyperpolarizations and the outward currents produced by dopamine was -109.7 +/- 1.7 mV (n = 12) when the potassium concentration was 2.5 mM and -74.0 +/- 5.0 mV (n = 4) when the potassium concentration was 10.5 mM. The change in reversal potentials in these and intermediate potassium concentrations was described by the Nernst equation. 4. The outward current induced by dopamine was reversibly reduced by barium (100-300 microM) and by high concentrations of tetraethylammonium (greater than or equal to 10 mM). Calcium-free solutions with cobalt (0.5-2 mM) did not reduce the current in response to dopamine during the first 5 min of their application. Currents and hyperpolarizations caused by dopamine were unaffected by tetrodotoxin (1 microM). 5. The hyperpolarization produced by dopamine was mimicked by the D2 receptor agonist quinpirole (LY 171555, 0.1-3 microM) and was blocked by the D2 receptor agonists domperidone and (-)-sulpiride. Agonists and antagonists at D1 receptors had no effect. 6. (-)-Sulpiride (30 nM-30 microM) produced a progressive shift to the right in the concentration-response curve to either dopamine or quinpirole. Schild analysis of the antagonism between (-)-sulpiride and quinpirole suggested competitive antagonism with a dissociation equilibrium constant for (-)-sulpiride of about 13 nM. 7. It is concluded that dopamine acts on D2 receptors on neurones of the rat substantia nigra pars compacta to increase the membrane potassium conductance.

Lacey, M G; Mercuri, N B; North, R A

1987-01-01

135

Impact of feline zona pellucida glycoprotein B-derived synthetic peptides on in vitro fertilization of cat oocytes.  

PubMed

Although immunocontraception based on porcine zona pellucida (ZP) proteins is widely applied in many species, it is not suitable for cat contraception due to the lack of cross-reactivity. Since the first ZP gene expressed during oocyte growth in domestic cats is ZPB, we assumed that immunization with feline ZPB (fZPB)-derived synthetic peptides may cause irreversible infertility, which would be preferable in stray cats. Thus, the present study evaluated the immunogenicity and the contraceptive potential of synthetic fZPB peptides. Antigenic epitope sequences were detected via epitope mapping using specific rabbit anti-fZP antibodies. Six peptides representing the recognized epitopes were synthesized subsequently. Two out of six peptides (ZPB amino acid residue 130-149 = P3 and 175-193 = P6) cross-reacted with anti-fZP antiserum in dot blot analysis and ELISA. Coupled to BSA, both peptides were utilized to produce specific antibodies in rats. Despite several booster injections the antibody titers monitored by ELISA did not exceed 1:5000. Both rat antisera were tested for contraceptive potential in cat in vitro maturation/in vitro fertilization (IVF). Antiserum against peptide P3 significantly inhibited sperm binding and fertilization of cat oocytes in vitro (57.3% of sperm binding; 41.5% of fertilization), whereas the inhibition by anti-P6 was not significant. Pre-incubation of sperm cells with both peptides before IVF failed to affect either sperm binding or fertilization (22.3 +/- 3.7 sperm/egg vs 25.5 +/- 5.8 for P3 and 20.7 +/- 4.0 for P6, respectively). In conclusion, antibodies directed against one of the two identified antigenic determinants of fZPB inhibited sperm binding and IVF and therefore showed promising results as a contraceptive. However, the specific immune response and anti-fertile properties of this synthetic vaccine have to be examined in vivo to verify the suitability of its components. PMID:15056783

Ringleb, Jennifer; Rohleder, Marlies; Jewgenow, Katarina

2004-02-01

136

Inoculation of female American black bears (Ursus americanus) with partially purified porcine zona pellucidae limits cub production.  

PubMed

The present 2-year study investigated the feasibility of using porcine zona pellucidae (pZP) as antigen for immunocontraception in American black bears. Sows, 3-6 years of age, were administered either two doses of 250 microg pZP with Freund's adjuvant (n = 10) or adjuvant alone (n = 5), one in April and one in May, and were kept away from the boars until June. Serum samples were collected before injections and before denning (November). The presence of sows with cubs at side was observed during premature emergence from denning. First-year results indicated that anti-pZP antibody titres in vaccinated sows were 2.5-9.0-fold (range) higher compared with non-vaccinated sows and that the vaccinated sows were threefold less likely to become pregnant (P = 0.167). Control and vaccinated bears produced 1.6 and 0.2 cubs per sow, respectively (P = 0.06). The second-year study investigated the feasibility of using pZP sequestered in a controlled-release pellet and a water-soluble adjuvant (QS-21) to avoid regulatory problems associated with Freund's adjuvant. Sows in the treatment group (n = 22) were administered a single dose of an emulsion of 250 microg pZP and 150 microg QS-21 plus a pellet containing 70-90 microg pZP for delayed release as booster dose. Control sows (n = 5) received the QS-21 adjuvant in pellet alone. Serum samples were collected before inoculations (April) and before denning (November). Seven cubs were born to the five control sows, but none was born to the 22 vaccinated sows (P < 0.001). Anti-pZP antibody mean absorbance ratios in control sows remained at background levels, whereas vaccinated sows had ratios fourfold higher than controls. Two-dimensional polyacrylamide gel electrophoresis and immunohistochemical localisation confirmed immunoreactivity of sera from inoculated bears. We conclude that cub production in the American black bear can be effectively limited with either two injections of 250 microg pZP or a single inoculation of partially purified pZP sequestered in controlled-release pellets. PMID:17601409

Lane, V M; Liu, I K M; Casey, K; vanLeeuwen, E M G; Flanagan, D R; Murata, K; Munro, C

2007-01-01

137

Shear stress induces preimplantation embryo death that is delayed by the zona pellucida and associated with stress-activated protein kinase-mediated apoptosis.  

PubMed

In this study, we discovered that embryos sense shear stress and sought to characterize the kinetics and the enzymatic mechanisms underlying induction of embryonic lethality by shear stress. Using a rotating wall vessel programmed to produce 1.2 dynes/cm2 shear stress, it was found that shear stress caused lethality within 12 h for E3.5 blastocysts. Embryos developed an approximate 100% increase in mitogen-activated protein kinase 8/9 (formerly known as stress-activated protein kinase/junC kinase 1/2) phosphorylation by 6 h of shear stress that further increased to approximately 350% by 12 h. Terminal deoxynucleotidyltransferase dUTP nick end labeling/apoptosis was at baseline levels at 6 h and increased to approximately 500% of baseline at 12 h, when irreversible commitment to death occurred. A mitogen-activated protein kinase 8/9 phosphorylation inhibitor, D-JNKI1, was able to inhibit over 50% of the apoptosis, suggesting a causal role for mitogen-activated protein kinase 8/9 phosphorylation in the shear stress-induced lethality. The E2.5 (compacted eight-cell/early morula stage) embryo was more sensitive to shear stress than the E3.5 (early blastocyst stage) embryo. Additionally, zona pellucida removal significantly accelerated shear stress-induced lethality while having no lethal effect on embryos in the static control. In conclusion, preimplantation embryos sense shear stress, chronic shear stress is lethal, and the zona pellucida lessens the lethal and sublethal effects of shear stress. Embryos in vivo would not experience as high a sustained velocity or shear stress as induced experimentally here. Lower shear stresses might induce sufficient mitogen-activated protein kinase 8/9 phosphorylation that would slow growth or cause premature differentiation if the zona pellucida were not intact. PMID:16571875

Xie, Yufen; Wang, Fangfei; Zhong, Wenjing; Puscheck, Elizabeth; Shen, Hayley; Rappolee, D A

2006-03-29

138

Endothelin1[1–31], acting as an ETA-receptor selective agonist, stimulates proliferation of cultured rat zona glomerulosa cells  

Microsoft Academic Search

Endothelin-1 (ET-1)[1–31] is a novel hypertensive peptide that mimics many of the vascular effects of the classic 21 amino acid peptide ET-1[1–21]. However, at variance with ET-1[1–21] that enhances aldosterone secretion from cultured rat zona glomerulosa (ZG) cells by acting via ETB receptors, ET-1[1–31] did not elicit such effect. Both ET-1[1–21] and ET-1[1–31] raised the proliferation rate of cultured ZG

Giuseppina Mazzocchi; Gian Paolo Rossi; Ludwik K. Malendowicz; Hunter C. Champion; Gastone G. Nussdorfer

2000-01-01

139

Zona pellucida glycoprotein mZP3 produced in milk of transgenic mice is active as a sperm receptor, but can be lethal to newborns  

Microsoft Academic Search

Mouse egg zona pellucida glycoprotein mZP3 (~83 kDa?Mr) serves as a species-specific sperm receptor and acrosome reaction-inducer during fertilization in mice. These biological activities are dependent on certain mZP3 serine\\/threonine- (O-) linked oligosaccharides present at the combining-site for sperm. In an attempt to produce large amounts of biologically active mZP3, we generated several transgenic mouse lines carrying the full-length mZP3

Eveline S. Litscher; Chengyu Liu; Yann Echelard; Paul M. Wassarman

1999-01-01

140

Control del cáncer y salud mundial: noticia del Instituto Nacional del Cáncer (NCI)  

Cancer.gov

En combinación con una reunión de alto nivel de las Naciones Unidas sobre enfermedades no transmisibles en países en vías de desarrollo, el doctor Harold Varmus, director del NCI, y el doctor Ted L. Trimble, del NCI, han publicado un comentario en Science Translational Medicine sobre “La integración del control del cáncer en la salud mundial" (Integrating Cancer Control into Global Health).

141

Seroprevalence and risk factors of toxoplasmosis in cattle from extensive and semi-intensive rearing systems at Zona da Mata, Minas Gerais state, Southern Brazil  

PubMed Central

Background Concerning the infection of humans by T. gondii, limited efforts have been directed to the elucidation of the role of horizontal transmission between hosts. One of the main routes of transmission from animals to humans occurs through the ingestion of raw or insufficiently cooked meat. However, even though the detection of T. gondii in meat constitutes an important short-term measure, control strategies can only be accomplished by a deeper understanding of the epidemiology of toxoplasmosis. The present study aimed to investigate the seroprevalence of toxoplasmosis in cattle from Zona da Mata, Minas Gerais, Brazil, and to identify associated risk factors, through an epidemiological investigation. Methods The animals studied (Bos indicus, breed Nelore or Gir) were reared in the Zona da Mata micro-region and killed at a commercial slaughterhouse at Juiz de Fora, Minas Gerais state. The animals came from 53 cattle farms with extensive (predominantly pasture feeding management) or semi-intensive (food management based on grazing, salt mineral and feed supplementation) rearing systems. Blood samples were collected from 1200 animals, and assigned to Indirect Fluorescent Antibody Test. Results When analyzing IgG anti-T.gondii we found an overall seroprevalence of 2.68%. In Brazil prevalences vary from 1.03% to 60%. Although in the present study, the seroprevalence per animal is considered low compared to those observed in other studies, we found out that of the 53 farms analyzed, 17 (34.69%) had one or more positive cattle. It is a considerable percentage, suggesting that the infection is well distributed through the Zona da Mata region. The results of the epidemiological investigation showed that the main risk factors of Toxoplasma gondii infection are related to animal management and to the definive host. There was a relationship between the number of seropositive cattle and the presence and number of resident cats, presence and number of stray cats, presence of cats walking freely, rat control by using cats and feed storage. Conclusion These results may contribute to the development of preventive strategies in Brazil and other developing countries were extensive and semi-intensive cattle rearing systems are very widespread and the efforts to control this important zoonotic disease have attained little success.

2013-01-01

142

Boletín del Instituto Nacional del Cáncer - 02-02-2010  

Cancer.gov

En un estudio clínico en fase inicial que probó una vacuna terapéutica en pacientes con leucemia mieloide crónica (LMC) que tomaban el fármaco imatinib (Gleevec), no se pudieron detectar células cancerosas en 7 de los 19 participantes por un tiempo promedio de 22 meses. Los resultados del estudio fueron publicados en el número del 1º de enero de la revista Clinical Cancer Research.

143

MMP2 and acrosin are major proteinases associated with the inner acrosomal membrane and may cooperate in sperm penetration of the zona pellucida during fertilization.  

PubMed

Sperm-zona pellucida (ZP) penetration during fertilization is a process that most likely involves enzymatic digestion of this extracellular coat by spermatozoa. Since the inner acrosomal membrane (IAM) is the leading edge of spermatozoa during penetration and proteins required for secondary binding of sperm to the zona are present on it, the IAM is the likely location of these enzymes. The objectives of this study were to identify and characterize proteinases present on the IAM, confirm their localization and provide evidence for their role in fertilization. Gelatin zymography of detergent extracts of the IAM revealed bands of enzymatic activity identified as serine and matrix metallo-proteinases (MMPs). Specific inhibitors to MMPs revealed that MMP activity was due to MMP2. Immunoblotting determined that the serine protease activity on the zymogram was due to acrosin and also confirmed the MMP2 activity. Immunogold labeling of spermatozoa at the electron microscope level showed that acrosin and MMP2 were confined to the apical and principal segments of the acrosome in association with the IAM, confirming our IAM isolation technique. Immunohistochemical examination of acrosin and MMP2 during spermiogenesis showed that both proteins originate in the acrosomic granule during the Golgi phase and later redistribute to the acrosomal membrane. Anti-MMP2 antibodies and inhibitors incorporated into in vitro fertilization media significantly decreased fertilization rates. This is the first study to demonstrate that MMP2 and acrosin are associated with the IAM and introduces the possibility of their cooperation in enzymatic digestion of the ZP during penetration. PMID:22729485

Ferrer, Marvin; Rodriguez, Hilma; Zara, Lindsay; Yu, Yang; Xu, Wei; Oko, Richard

2012-05-22

144

Cáncer de cuello del útero o uterino  

Cancer.gov

Información acerca del cáncer de cérvix (cervical o cuello uterino), lo cual incluye temas como tratamiento, prevención, causas, factores de riesgo, exámenes selectivos de detección, estudios clínicos y estadísticas del Instituto Nacional del Cáncer.

145

EL ORIGEN BIOLÓGICO DEL DERECHO  

Microsoft Academic Search

En este trabajo se aborda el posible origen evolutivo del derecho como una consecuencia del desarrollo social de nuestra especie. Otras especies con sistemas sociales similares presentan varias reglas para la convivencia en grupo. Algunas de estas reglas son comunes en todas las especies con comportamiento social. El desarrollo de la sociobiología en las últimas décadas es una herramienta importante

Axel P. Retana-Salazar

146

Alberto del Pozo  

NSDL National Science Digital Library

The Cuban Heritage Collection of the University of Miami Libraries was a recipient of 17 original illustrations by Alberto del Pozo in 1998 from the Campilli family. "The Oricha Collection" is their gift, and it contains illustrations in pen, crayon, and ink and the collection "pays homage to the primary gods and goddesses that comprise the Afro-Cuban religion of Santer'a." The "Introduction" near the top of the page, is a good place for visitors to start learning about Pozo, who was a Parson's School of Design-trained, Cuban artist who died at the age of 47. The "Biographical Sketch" is worth looking at as well. Examples of his colorful, heavily patterned and symbolism-intensive drawings of Santeria gods and goddesses can be found in the link "Gallery". The description of each drawing is given in English and Spanish. Finally, visitors interested in more information on the Santeria, can look at the "Further Readings" link for authoritative books on the subject.

147

Sulla struttura eletronica del nitrobenzolo  

Microsoft Academic Search

Riassunto  È presentato un calcolo di alcune grandezze fisiche del nitrobenzolo mediante il metodo M.O. con una ragionevole scelta dei\\u000a parametri necessari. Per il momento elettrico, la frequenza del primo massimo di assorbimento nell’ultravioletto, le densità\\u000a elettroniche, gli ordini di legame, gli indici di valenza libera e l’energia di risonanza si ottengono valori in ottimo accordo\\u000a con le previsioni. Anche il

M. Simonetta; A. Vaciago

1954-01-01

148

Effects of glutathione (GSH and GSSG) and glutathione reductase (GR) on zona-free hamster oocyte ability to decondense human sperm.  

PubMed

Effects of reduced glutathione (GSH), oxidized glutathione (GSSG), or glutathione reductase (GR) supply were studied on the ability of hamster oocytes to be fertilized by human sperm. Zona-free oocytes were pretreated with these compounds prior to sperm insemination. Oocyte pretreatment with high concentrations of GSH or GSSG (50 or 100 mM, 30 min) significantly increased the penetrated oocyte rate (PR). Polyspermy was not increased except when high concentrations of GSH (100 mM) were used. Incubation of oocytes with GR (1 or 10 IU/ml) prior to sperm insemination induced increasing dose-dependent PR. Polyspermy increased significantly with 10 mM GR in oocyte incubation medium. Oocyte incubation for 30 min with the sulfhydryl blocking agent iodoacetamide (1 mM) led to a drastic decrease in oocyte penetration and in polyspermy. Our results demonstrate an original way to increase the efficacy of human-hamster heterospecific fertilization. Various hypotheses are discussed explaining these observations which open new investigations for heterospecific and homospecific in vitro fertilization. PMID:2591849

Lassalle, B; Testart, J

1989-09-01

149

Tuberoinfundibular Peptide of 39 Residues-Immunoreactive Fibers in the Zona Incerta and the Supraoptic Decussations Terminate in the Neuroendocrine Hypothalamus  

PubMed Central

Tuberoinfundibular peptide of 39 residues (TIP39) is expressed by neurons in the subparafascicular area, the posterior intralaminar complex of the thalamus and the pontine medial paralemniscal nucleus. TIP39-positive fibers from these areas do not form individual bundles or fascicles, they join other pathways to reach their innervated brain areas. Fibers arise from TIP39 perikarya located in the subparafascicular area and the posterior intralaminar complex of the thalamus could be followed to the hypothalamus. After uni- and bilateral posterolateral surgical deafferentations of the hypothalamus, accumulation of TIP39 immunoreactivity was observed in the fibers caudal to the knife cut, while it disappeared completely rostral to the transection. In serial sections of the forebrain, we could follow TIP39-ir fibers coursing within the zona incerta and the supraoptic decussations. TIP39-positive fibers that join the incerto-hypothalamic pathway reach the medio-dorsal part of the hypothalamus and form moderate to high density networks in the dorsomedial and paraventricular nuclei. The other set of TIP39-positive axons from the subthalamic area join the fibers of the supraoptic decussations and run in an antero-medial direction through the most ventral portion of the hypothalamus up to the retrochiasmatic area, where they crossover. A certain portion of these TIP39-positive fibers terminates in the territories of the arcuate and the medial preoptic nuclei, as well as in the retrochiasmatic area.

Usdin, T. B.; Makara, G. B.; Dobolyi, A.

2012-01-01

150

Phage display allows identification of zona pellucida-binding peptides with species-specific properties: novel approach for development of contraceptive vaccines for wildlife.  

PubMed

Multiple phage-peptide constructs, where the peptides mimic sperm epitopes that bind to zona pellucida (ZP) proteins, were generated via selection from a phage display library using a novel approach. Selections were designed to allow for identification of ZP-binding phage clones with potential species-specific properties, an important feature for wildlife oral vaccines as the goal is to control overpopulation of a target species while not affecting non-target species' reproduction. Six phage-peptide antigens were injected intramuscularly into pigs and corresponding immune responses evaluated. Administration of the antigens into pigs stimulated production of anti-peptide antibodies, which were shown to act as anti-sperm antibodies. Potentially, such anti-sperm antibodies could interfere with sperm delivery or function in the male or female genital tract, leading to contraceptive effects. Staining of semen samples collected from different mammalian species, including pig, cat, dog, bull, and mouse, with anti-sera from pigs immunized with ZP-binding phage allowed identification of phage-peptide constructs with different levels of species specificity. Based on the intensity of the immune responses and specificity of these responses in different species, two of the antigens with fusion peptide sequences GEGGYGSHD and GQQGLNGDS were recognized as the most promising candidates for development of contraceptive vaccines for wild pigs. PMID:23079080

Samoylova, Tatiana I; Cochran, Anna M; Samoylov, Alexandre M; Schemera, Bettina; Breiteneicher, Adam H; Ditchkoff, Stephen S; Petrenko, Valery A; Cox, Nancy R

2012-10-16

151

VariaciÛn espacial y temporal de la riqueza florÌstica de macroalgas en la zona rocosa de BahÌa de Muertos, B.C.S. MÈxico  

Microsoft Academic Search

RÈsumÈ VariaciÛn espacial y temporal de Temporal and spatial variation of Variation spatiale et temporelle la riqueza florÌstica de macroalgas the seaweed richness in the rocky de la richesse floristique de macro en la zona rocosa de BahÌa de zone at BahÌa de Muertos, B.C.S., algues dans la zone rocheuse de la Muertos, B.C.S. MÈxico. Se realizÛ MÈxico. The temporal

Georgina Saad-Navarro; Rafael Riosmena-RodrÌguez

152

FENOLOGÍA DEL CULTIVO DEL CHILE (CAPSICUM ANNUUM L.)  

Microsoft Academic Search

RESUMEN Datos diarios de temperaturas máximas y mínimas del aire fueron usados para determinar las unidades calor, que sirvieron como referencia para definir las principales etapas fenológicas en seis tipos diferentes de chile (Capsicum annuum). Las unidades calor sirvieron para inferir el período de mayor requerimiento de nutrimentos, así como la relación con la sintomatología de las enfermedades más importantes

Salvador Montes Hernández; Elena Heredia García; J. Alfonso; Aguirre Gómez

153

Dieta del pez espada Xiphias gladius Linnaeus, 1758 en distintas zonas de pesca frente a Chile central durante el otoño de 2004 Diet of swordfish Xiphias gladius Linnaeus, 1758 from different fishing zones off central-Chile during autumn 2004  

Microsoft Academic Search

The habitat of the predator and sample size (stomach number) are some of the most important factors to consider in the study of its diet. To investigate these variations we studied the diet of 51 swordfish Xiphias gladius (range: 173-301 cm) in four fishing areas off central Chile (30ºS - 40ºS) during autumn-winter of 2004. Frequency of occurrence, numeric and

Katherine Castillo; Christian M. Ibáñez; Carlos González; Javier Chong

154

Rodent middens, a new method for Quaternary research in arid zones of South America [Paleomadrigueras de roedores, un nuevo me??todo para el estudio del Cuaternario en zonas a??ridas de Sudame??rica  

USGS Publications Warehouse

In arid and semi-arid regions of South America, historical evidence for climate and vegetation change is scarce despite its importance for determining reference conditions and rates of natural variability in areas susceptible to modern desertification. Normal lines of evidence, such as pollen stratigraphies from lakes, are either rare or unobtainable in deserts; studies of late Quaternary vegetation history are few and generally inconclusive. This gap in knowledge may be corrected with discovery and development of fossil rodent middens in rocky environments throughout arid South America. These middens, mostly the work of Lagidium, Phyllotis, Abrocoma and Octodontomys, are rich in readily identifiable plant macrofossils, cuticles and pollen, as well as vertebrate and insect remains. In the North American deserts, more than 2,500 woodrat (Neotoma) middens analyzed since 1960 have yielded a detailed history of environmental change during the past 40,000 years. Preliminary work in the pre-puna, Monte and Patagonian Deserts of western Argentina, the Atacama Desert of northern Chile/southern Peru, the Mediterranean matorral of central Chile, and the Puna of the Andean altiplano suggest a similar potential for rodent middens in South America. Here we borrow from the North American experience to synthesize methodologies and approaches, summarize preliminary work, and explore the potential of rodent midden research in South America.

Betancourt, J. L.; Saavedra, B.

2002-01-01

155

Cuantificacion de plomo en leche cruda que se produce en algunas zonas del Valle de Toluca mediante tecnicas analiticas. (Determination of the concentrations of lead in raw milk in the Valley of Toluca).  

National Technical Information Service (NTIS)

The object of the present work, undertaken under the program of Regional and Cooperative Agreement for Nuclear Science and Technology in Latin America (ARCAL IV), was to determine the amounts of lead found in raw milk using the analytic techniques of Atom...

E. Rosas Vilchis

1991-01-01

156

Space as a Metafictional Marker: Borges' 'Tema del traidor y del héroe' and Bertolucci's Strategia del ragno  

Microsoft Academic Search

:In this study, I analyse space as a key marker of the metafictional characteristic of Jorge Luis Borges' story 'Tema del traidor y del héroe' (in Ficciones) and Bernardo Bertolucci's film Strategia del ragno. I contend that the presentation of space – this understudied 'other' with regard to time in criticism of Borges – is a major aspect of the

Ilka Kressner

157

Space as a Metafictional Marker: Borges' 'Tema del traidor y del héroe' and Bertolucci's Strategia del ragno  

Microsoft Academic Search

In this study, I analyse space as a key marker of the metafictional characteristic of Jorge Luis Borges' story 'Tema del traidor y del héroe' (in Ficciones) and Bernardo Bertolucci's film Strategia del ragno. I contend that the presentation of space – this understudied 'other' with regard to time in criticism of Borges – is a major aspect of the

Ilka Kressner

158

Considerazioni sulla proprieta' insulino-legante del siero del soggetto normale e del diabetico mai trattato con insulina  

Microsoft Academic Search

Riassunto Gli AA. rilevano che i sieri dell'individuo normale e del diabetico mai trattato con insulina sono provvisti di proprietà insulino-legante di entità analoga. In alcuni sieri, sia del soggetto normale che del diabetico, è presente un'attività insulino-legante superiore ai valori massimi normali, che diminuisce dopo aggiunta di µU 500 di insulina bovina.

Michele Lunetta; Giuseppe Calcagno; Luciano Motta; Turiddu Lombardo

1968-01-01

159

Interference with the 19S proteasomal regulatory complex subunit PSMD4 on the sperm surface inhibits sperm-zona pellucida penetration during porcine fertilization.  

PubMed

Proteolysis of ubiquitinated sperm and oocyte proteins by the 26S proteasome is necessary for the success of mammalian fertilization, including but not limited to acrosomal exocytosis and sperm-zona pellucida (ZP) penetration. The present study examined the role of PSMD4, an essential non-ATPase subunit of the proteasomal 19S regulatory complex responsible for proteasome-substrate recognition, in sperm-ZP penetration during porcine fertilization in vitro (IVF). Porcine sperm-ZP penetration, but not sperm-ZP binding, was blocked in the presence of a monoclonal anti-PSMD4 antibody during IVF. Inclusion in the fertilization medium of mutant ubiquitins (Ub+1 and Ub5+1), which are refractory to processing by the 19S regulatory complex and associated with Alzheimer's disease, also inhibited fertilization. This observation suggested that subunit PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. Immunofluorescence localized PSMD4 in the sperm acrosome. Immunoprecipitation and proteomic analysis revealed that PSMD4 co-precipitated with porcine sperm-associated acrosin inhibitor (AI). Ubiquitinated species of AI were isolated from boar sperm extracts by affinity purification of ubiquitinated proteins using the recombinant UBA domain of p62 protein. Some proteasomes appeared to be anchored to the sperm head inner acrosomal membrane, as documented by co-fractionation studies. In conclusion, the 19S regulatory complex subunit PSMD4 is involved in the sperm-ZP penetration during fertilization. The recognition of substrates on the ZP by the 19S proteasomal regulatory complex is essential for the success of porcine/mammalian fertilization in vitro. PMID:20526895

Yi, Young-Joo; Manandhar, Gaurishankar; Sutovsky, Miriam; Zimmerman, Shawn W; Jonáková, Vera; van Leeuwen, Fred W; Oko, Richard; Park, Chang-Sik; Sutovsky, Peter

2010-06-08

160

Immunization with recombinant murine cytomegalovirus expressing murine zona pellucida 3 causes permanent infertility in BALB/c mice due to follicle depletion and ovulation failure.  

PubMed

Zona pellucida (ZP) glycoproteins are promising candidate antigens for use in immunocontraceptive vaccines because of their crucial role in mammalian fertilization. A single intraperitoneal immunization with recombinant murine cytomegalovirus engineered to express murine ZP3 (rMCMV-mZP3) induces permanent infertility with no evident systemic illness in female BALB/c mice. To investigate the mechanisms underpinning reproductive failure elicited by rMCMV-mZP3, ovarian parameters and reproductive function were evaluated at time points spanning 10 days to 5 wk after virus inoculation. Fertility was substantially impaired by 14 days after inoculation with rMCMV-mZP3 and was fully ablated by 21 days. Pregnancies established after inoculation but before complete infertility showed no adverse effects on fetal viability assessed at Day 17.5 post coitum (pc). Infertile mice retained estrous cycling activity and remained receptive to mating; however, at Day 3.5 pc there were fewer developing embryos and corpora lutea, plasma progesterone content was reduced, and there was no evidence of excess unfertilized oocytes. Consistent with this, profound ovarian pathology was evident from 10 days after rMCMV-mZP3 inoculation, with a decline first in mature ovarian follicles and then in immature ovarian follicles and with diminished expression of genes regulating follicle development, including Nobox, Gdf9, and Gja1 (connexin43). Follicle loss was associated with mild focal oophoritis and with recruitment of inflammatory leukocytes, predominantly CD4(+) and CD8(+) T cells evident from 10 days after virus inoculation. These data indicate that vaccination with rMCMV-mZP3 causes permanent infertility in BALB/c mice principally due to induction of ovarian autoimmune pathology leading to progressive oocyte depletion and eventual ovulation failure. PMID:18667753

O'Leary, Sean; Lloyd, Megan L; Shellam, Geoffrey R; Robertson, Sarah A

2008-07-30

161

Expression of zona pellucida B proteins in juvenile rare minnow (Gobiocypris rarus) exposed to 17?-ethinylestradiol, 4-nonylphenol and bisphenol A.  

PubMed

Zona pellucida (ZP) containing proteins are glycoproteins in teleost chorion and are encoded by several gene subfamilies, mainly including ZPA, ZPB, ZPC and ZPX genes. In teleost species, ZP genes are expressed either in liver under regulation of estrogen or in ovary. In the present study, five ZP gene isoforms were isolated and characterized in Gobiocypris rarus. The putative amino acid sequences of these ZP gene isoforms contain the typical trefoil motif and a ZP domain. These five G. rarus ZP gene isoforms were named as grZPB.1, grZPB.2, grZPB.3, grZPB.4 and grZPB.5. Real-time quantitative reverse transcription PCR (RT-qPCR) analysis indicated that all these ZP mRNA isoforms were exclusively expressed in ovary. G. rarus juveniles at the age of 21 days postfertilization were exposed to 17?-ethinylestradiol (EE2; 0.01, 0.1 and 1 nM), 4-nonylphenol (4-NP; 10, 100 and 1000 nM) or bisphenol A (BPA; 0.1, 1 and 10nM) for 3 days. mRNA expressions of ZPB isoforms following the exposure to xenoestrogen were detected by RT-qPCR. Data were analyzed by the 2(-??Cq) method. The results indicate that induction by 0.1-1nM EE2 on mRNA expression of the grZPB isoforms is weaker than for vitellogenin. 4-NP exposures at three concentrations had differential effects on the grZPBs. BPA at three concentrations weakly induced mRNA expression of the grZPB isoforms. PMID:21946248

Wu, Tingting; Wang, Houpeng; Qin, Fang; Liu, Shaozhen; Li, Meng; Xu, Peng; Wang, Zaizhao

2011-09-17

162

Marina Del Rey Subregional Plan.  

National Technical Information Service (NTIS)

The Marina del Ray Subregional Plan was created for submission to the South Coast Regional Commission of the California Coastal Zone Conservation Commission. Since there are not, as yet, any other coastal subregional plans to use as examples, this plan fo...

M. McCoy C. Walecka

1975-01-01

163

Del Mar College GIS Program  

NSDL National Science Digital Library

Del Mar College offers a comprehensive geospatial program with certificates and Associates degrees in GIS. This site includes description of the program and courses, along with further contact information. By clicking on the link for "Geographic Information Systems" you may download a PDF with information about the program.

2010-10-28

164

Del Pezzo surfaces with Du Val singularities  

NASA Astrophysics Data System (ADS)

In this paper we study the problem of existence of orbifold Kaehler-Einstein metrics on del Pezzo surfaces of degree 1 with Du Val singular points. Moreover we compute global log canonical thresholds of del Pezzo surfaces of degree 1 with Du Val singularities and of del Pezzo surfaces of Picard rank 1 with Du Val singularities.

Kosta, Dimitra

2009-04-01

165

Zona Land: Wave Interference 1  

NSDL National Science Digital Library

This applet demonstrates basic wave superposition through an interactive simulation. The user is able to select two sinusoidal waveforms, move them together, and view the resulting wave as the interference occurs. Brief explanations are offered to help better explain what is happening on the screen.

Zobel, Edward

2008-08-10

166

Zona Land: Wave Interference 2  

NSDL National Science Digital Library

This applet demonstrates basic wave superposition through an interactive simulation. The user is able to select two sinusoidal waveforms, move them together, and view the resulting wave as the interference occurs. Brief explanations are offered to help better explain what is happening on the screen.

Zobel, Edward

2008-06-24

167

Zona Land: Wave Interference 3  

NSDL National Science Digital Library

This applet demonstrates basic wave superposition. The user can draw two waveforms and view the resulting wave on a string as they move in opposite directions towards the middle of the string. The user can select to view each of the individual waves, the sum, or both. The simulation can be slowed down to help the user examine the phenomenon more closely.

Zobel, Edward

2008-08-10

168

Localization of vascular endothelial growth factor in the zona pellucida of developing ovarian follicles in the rat: a possible role in destiny of follicles.  

PubMed

There is increasing evidence that in many species angiogenic factors, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), may have important roles in folliculogenesis. The aim of this study is to determine the localization of VEGF and its receptors, Flt-1 and KDR, and bFGF expression in the rat ovary and to evaluate their distributions throughout the different follicular stages. Out of 20 virginal female rats, 10 were studied during the natural ovarian cycle without any ovulation induction. The other 10 were superovulated and their ovaries were studied by western analysis and immunohistochemistry. Granulosa cells (GC) and oocytes of primordial follicles were negative for VEGF. In early primary follicles, VEGF was present in the oocyte but its immunoreactivity was weak, while newly developing zona pellucida (ZP) of primary follicles was negative for VEGF. Subsequently, with the commencement of antral spaces between GC of the secondary follicle, ZP of some secondary follicles became strongly positive for VEGF, forming a continuous ring around the oocyte. In preovulatory mature follicles granulosa and theca interna (TI) cells showed a weak immunoreactivity for VEGF. Western blot analyses have also demonstrated that VEGF, a 26-kDa protein, was present in follicles. Moreover, in ovulated cumulus-oocyte complex we observed a halo-like immunoreactivity of VEGF around the fully mature oocyte. The immunoreactivity for Flt-1 and KDR receptors in growing follicles was mostly limited to GC and TI cells. Anti-bFGF did not exhibit any immunoreactivity in ZP of follicles at any stage. Its expression was weak in GC of the follicles at different stages, whereas, it could be localized to some extent in the blood capillaries of TI of antral follicles and in blood vessels localized in the stroma. Interestingly, VEGF immunoreactivity in the ZP of some secondary follicles is very striking. Accordingly, the possibility that VEGF may be an important regulatory molecule for the dominant follicle selection or atresia should be considered. PMID:14605899

Celik-Ozenci, Ciler; Akkoyunlu, Gokhan; Kayisli, Umit Ali; Arici, Aydin; Demir, Ramazan

2003-11-06

169

Política de comentarios del NCI  

Cancer.gov

Animamos a los usuarios a compartir sus ideas relacionadas con los temas en discusión en las secciones o foros determinados para tal fin en este sitio web u otros sitios administrados por el Instituto Nacional del Cáncer. Revisamos y publicamos los comentarios de acuerdo a la política indicada a continuación. Nos reservamos el derecho, a nuestra completa discreción, a no publicar o eliminar comentarios que no están de acuerdo con nuestra política.

170

DelPhi: a comprehensive suite for DelPhi software and associated resources  

PubMed Central

Background Accurate modeling of electrostatic potential and corresponding energies becomes increasingly important for understanding properties of biological macromolecules and their complexes. However, this is not an easy task due to the irregular shape of biological entities and the presence of water and mobile ions. Results Here we report a comprehensive suite for the well-known Poisson-Boltzmann solver, DelPhi, enriched with additional features to facilitate DelPhi usage. The suite allows for easy download of both DelPhi executable files and source code along with a makefile for local installations. The users can obtain the DelPhi manual and parameter files required for the corresponding investigation. Non-experienced researchers can download examples containing all necessary data to carry out DelPhi runs on a set of selected examples illustrating various DelPhi features and demonstrating DelPhi’s accuracy against analytical solutions. Conclusions DelPhi suite offers not only the DelPhi executable and sources files, examples and parameter files, but also provides links to third party developed resources either utilizing DelPhi or providing plugins for DelPhi. In addition, the users and developers are offered a forum to share ideas, resolve issues, report bugs and seek help with respect to the DelPhi package. The resource is available free of charge for academic users from URL: http://compbio.clemson.edu/DelPhi.php.

2012-01-01

171

Ayuda para usar el sitio web en español del Instituto Nacional del Cáncer  

Cancer.gov

Página de guía que le permite al lector entender la forma en que está organizado el sitio web del Instituto Nacional del Cáncer (NCI), las categorías de información disponibles y las políticas que rigen este sitio web.

172

Los dilemas del desarrollo territorial: repercusiones del zapatismo en la Selva Lacandona de Chiapas1  

Microsoft Academic Search

Este artículo discute los dilemas del desarrollo territorial en la Selva Lacandona. En particular analiza las relaciones entre movimientos sociales, nuevas formas de gobernanza ambiental y desarrollo en esta región del estado de Chiapas, en México. Con base en el llamado enfoque territorial del desarrollo rural (Schejtman y Berdegué, 2004), planteamos que en el contexto actual de globalización, para lograr

Luis Reygadas

173

Zona pellucida glycoprotein 3 (pZP3) and integrin ?2 (ITGB2) mRNA and protein expression in porcine oocytes after single and double exposure to brilliant cresyl blue test.  

PubMed

Brilliant cresyl blues (BCB) staining test is a useful tool in assessing the competence of cumulus-oocyte-complexes (COCs) in several mammalian species. It is mostly used to select gametes after they are recovered from the ovary or before and after IVM to isolate those oocytes that reach developmental competency. However, there is evidence that double exposure to BCB test may lead to impaired fertilization or even have a toxic effect on cells. The aim of the present study was to investigate the expression pattern of sperm-egg interaction molecules in oocytes after single and double exposure to BCB test. Follicles were dissected from porcine ovaries after slaughter and aspirated COCs were cultured in standard porcine IVM culture medium (TCM 199) for 44 h. The BCB test was applied to COCs before and after IVM. In developmentally competent oocytes, assessed by determining the activity of glucose-6-phosphate dehydrogenase (G6PDH; BCB test), real-time quantitative PCR reaction methods, western blot and confocal microscopy analysis were applied to determine the transcript levels of porcine zona pellucida glycoprotein 3 (pZP3), and integrin beta 2 (ITGB2), as well as the levels of pZP3 and ITGB2 proteins. In the control group, assessment of the expression of the investigated genes was performed before and after IVM without BCB test. We observed a significantly higher level of pZP3 mRNA in oocytes after single exposure to BCB test compared to control before and after IVM (P < 0.001), and to double staining (P < 0.05). The level of ITGB2 mRNA was also increased in gametes after single exposure to BCB test as compared to control before and after IVM (P < 0.001, P < 0.01, respectively), and double staining (P < 0.05). Western blot analysis demonstrated a higher level of pZP3 protein in oocytes after single staining with BCB as compared to control both before and after IVM (P < 0.001, P < 0.05, respectively) and double staining (P < 0.05). Confocal microscopic observations have revealed the same pattern of increased level of pZP3 and ITGB2 expression after single exposure to BCB test. In both cases we detected specific cytoplasmic localization of both proteins. The ITGB2 protein has zona pellucida and membrane localization in control oocytes before IVM. After IVM and after single exposure to BCB, ITGB2 was also strongly detected in the cytoplasm. In both cases, after double exposure to BCB both proteins were detected only partially in the cytoplasm. Our results suggest that (i) single exposure to BCB increased the expression of sperm-oocyte interaction genes, (ii) double exposure to BCB leads to only partial expression of pZP3 and ITGB2 in oocyte cytoplasm, (iii) the BCB staining test itself may be a cause of specific pZP3 translocation from the zona pellucida to the cytoplasm, and that (iv) in vitro maturation of oocytes may increase ITGB2 expression and translocation from the zona pellucida to the cytoplasm. PMID:21295838

Kempisty, B; Jackowska, M; Piotrowska, H; Antosik, P; Wo?na, M; Bukowska, D; Brüssow, K P; Ja?kowski, J M

2011-02-04

174

Aristocracia fantástica: los moderados y la poética del gobierno representativo  

Microsoft Academic Search

La mayor parte de los ideólogos del moderantismo tras la implantación del régimen liberal fueron destacados teóricos de la estética que contribuyeron a fijar el canon del romanticismo en España. Este artículo investiga la influencia de las reflexiones estéticas de autores como Alberto Lista, Nicomedes Pastor Díaz y Alcalá Galiano en su teoría del gobierno representativo. El asunto de fondo

Pablo Sánchez León

2006-01-01

175

33 CFR 80.1118 - Marina Del Rey, CA.  

Code of Federal Regulations, 2010 CFR

...2010-07-01 2010-07-01 false Marina Del Rey, CA. 80.1118 Section 80.1118 Navigation...LINES Pacific Coast § 80.1118 Marina Del Rey, CA. (a) A line drawn from Marina Del Rey Breakwater South Light 1 to Marina Del Rey...

2010-07-01

176

33 CFR 80.1118 - Marina Del Rey, CA.  

Code of Federal Regulations, 2010 CFR

...2009-07-01 2009-07-01 false Marina Del Rey, CA. 80.1118 Section 80.1118 Navigation...LINES Pacific Coast § 80.1118 Marina Del Rey, CA. (a) A line drawn from Marina Del Rey Breakwater South Light 1 to Marina Del Rey...

2009-07-01

177

Dust formation in Nova Del 2013  

NASA Astrophysics Data System (ADS)

We present the result of the IR photometry of Nova Del 2013 obtained at the Crimean Station (Nauchny, Ukraine) of the Sternberg Astronomical Institute. JHKLM photometry was carried out with 125-cm telescope using a InSb-photometer on:

Shenavrin, V. I.; Taranova, O. G.; Tatarnikov, A. M.

2013-09-01

178

Radio-Observaciones del OH EN la Coma del Cometa Halley Desde EL Hemisferio Sur  

NASA Astrophysics Data System (ADS)

Se utilizó una antena de 30 metros del Instituto Argentino de Radioastronomía para observaciones diarias Cf ebrero a abril de 1986) de la transición en 1667 MHz ( ? = 18 cm) del OH en la coma del cometa Halley. De las observaciones realizadas se concluye: 1) El número promedio de moléculas de OH en la coma durante 37 días de observación fue de (8.9±3.5)x1034 moléculas, lo que implica una tasa de producción promedio de OH de 1.8x1029 moléculas seg-1 y consecuentemente una pérdida de masa promedio de 17±6 toneladas seg-1 . Este valor está de acuerdo con las mediciones realizadas por las sondas Vega y Giotto. 2) El monitoreo desde el lAR revela la existencia de variaciones bruscas en los flujos de absorción del OH. Estas variaciones son consistentes con los modelos que representan la producción gaseosa a partir de ejecciones y/o desprendimientos discretos de materia congelada del núcleo. 3) Las variaciones en la densidad de flujo son consistentes con las estimaciones de los tiem- pos de vida medios del H2O y del OH en presencia del campo de radiación solar. 4) Se encuentra una correlación entre la intensidad del flujo absorbido y anisotropías en Ia dinamica de la coma.

Silva, A. M.; Bajaja, E.; Morras, R.; Cersosimo, J. C.; Martin, M. C.; Arnal, E. M.; Poppel, W. G. L.; Colomb, F. R.; Mazzaro, J.; Olalde, J. C.; Boriakoff, V.; Mirabel, I. F.

1987-05-01

179

Precio del agua y relocalización del recurso en la economía andaluza. Una aproximación desde un modelo de equilibrio general aplicado  

Microsoft Academic Search

El objetivo de este trabajo consiste en analizar los efectos que tendría un incremento en la tarifa del agua del sector agrario sobre la conservación del recurso, la eficiencia en el consumo y la posible relocalización del mismo entre los diferentes sectores productivos. La política tarifaria se aplicará sobre el sector agrario debido, por un lado, al excesivo consumo de

M. Alejandro Cardenete; G. J. D. Hewings; E. Velázquez

2005-01-01

180

Declaración del Instituto Nacional del Cáncer sobre los exámenes selectivos de detección de cáncer de seno  

Cancer.gov

El Instituto Nacional del Cáncer (NCI) agradece la cuidadosa revisión y análisis que ha hecho la Brigada de Servicios Preventivos de los Estados Unidos de la evidencia con respecto a los exámenes de detección de cáncer de seno para mujeres que tienen un riesgo del promedio.Preguntas y Repuestas

181

Boletín del Instituto Nacional del Cáncer - 3 de enero de 2012  

Cancer.gov

Dra. Rachel Ballard-Barbash Nuevos indicios del papel de la obesidad en el cáncer A medida que ha aumentado la prevalencia de la obesidad alrededor del mundo, se ha incrementado también la preocupación sobre sus consecuencias en la salud pública. Los índices de obesidad se han más que duplicado desde 1980, según la Organización Mundial de la Salud.

182

Preguntas y respuestas acerca del Estudio del Tamoxifeno y Raloxifeno (STAR)  

Cancer.gov

El Estudio del Tamoxifeno y Raloxifeno (STAR, por sus siglas en inglés) es un estudio clínico (un estudio de investigación conducido con voluntarios) diseñado para ver cómo el medicamento raloxifeno (Evista®) se compara con el medicamento tamoxifeno (Nolvadex®) en reducir la incidencia de cáncer del seno en las mujeres con alto riesgo de desarrollar la enfermedad.

183

Intertextual Sexual Politics: Illness and Desire in Enrique Gomez Carrillo's "Del amor", "del dolor y del vicio" and Aurora Caceres's "La rosa muerta"  

ERIC Educational Resources Information Center

|This study explores the intertextuality between Aurora Caceres's "La rosa muerta" (1914) and the novel "Del amor, del dolor y del vicio" (1898) by her ex-husband, Enrique Gomez Carrillo. Caceres strategically mentions Gomez Carrillo's novel in "La rosa muerta" to invite a reading of her work in dialogue with his. Both narratives follow the sexual…

LaGreca, Nancy

2012-01-01

184

Evaluación de elementos estructurales del hábitat del borrego cimarrón en la Sierra del Mechudo, Baja California Sur, México  

Microsoft Academic Search

Habitat lost and fragmentation can result in an isolated and local extinction risk for small populations like Ovis canadensis weemsi Goldman, 1937, in Sierra del Mechudo, Baja California Sur, Mexico. That is why it is important to evaluate the relationship between species and their habitat in order to design management and conservation plans. The object of this study was to

Sergio Alvarez-Cárdenas; Patricia Galina-Tessaro; Sara Díaz-Castro; Israel Guerrero-Cárdenas; Aradit Castellanos-Vera; Erika Mesa-Zavala

185

Near Infrared Photometry of Nova Del 2013  

NASA Astrophysics Data System (ADS)

We continued to follow Nova Del 2013 during the period from 31.15 August 2013 UT through 25.18 September 2013 UT using an As:Si bolometer mounted on the 0.76-m infrared telescope of the University of Minnesota's O'Brien Observatory (Marine on St. Croix, Minnesota, USA). Vega (alpha Lyrae) is used as the standard star.

Cass, A. C.; Carlon, R. L.; Corgan, D. T.; Dykhoff, D. A.; Gehrz, R. D.; Shenoy, D. P.

2013-09-01

186

Andrea del Sarto rehabilitated: a psychoanalytic emendation.  

PubMed

Ernest Jones's "The Influence of Andrea del Sarto's Wife on His Art" (1913) is an early example of psychoanalysis applied to the study of a prominent painter. Greatly influenced by Freud's Leonardo da Vinci and a Memory of His Childhood, Jones gave excessive credence to Vasari's highly prejudicial account of the life of del Sarto, on which the study relied heavily. Jones attempted to account psychologically for the circumstance that del Sarto, though highly skilled and "faultless," was not the equal of the three preeminent masters of the Italian High Renaissance: Leonardo, Michelangelo, and Raphael. Jones's uncritical acceptance of the Vasari biography encouraged him to view Sarto's assumed deficiency as the result of excessive attachment to his wife, a pathological uxoriousness. A contemporary psychoanalytic perspective, with its emphasis on the emotive response of the analyst, requires us to pay attention to the evocative nature of the work of the artist, an approach Jones neglected. In an examination of several paintings, the artist's sensitivity to the position of the spectator is explored, as is the interest in involving the viewer spatially and emotionally. An appreciation for the viewer's position is consistent with a capacity for using projected internal objects for creative purposes. The presence of this capacity suggests a revised view of del Sarto's contribution to art and of his relationship with his wife. PMID:12580329

Trosman, Harry

2002-01-01

187

Quina és la funció del gen clonat?  

Microsoft Academic Search

Presentem una activitat didàctica complementària per a alumnes de segon de batxillerat. A partir d’una seqüència de nucleòtids del genoma de Paenibacillus barcinonensis determinem la regió codificant d’un gen, la proteïna corresponent i la seva funció. Com a annex s’inclou el guió per a l’alumnat.

Margarita Soriano; Francisca Guerola

2008-01-01

188

ECONOMÍAS EXTERNAS Y LOCALIZACIÓN DEL EMPLEO INDUSTRIAL  

Microsoft Academic Search

Es un hecho fácilmente constatable que la actividad económica en gene- ral e industrial en particular se localiza de forma desigual a lo largo de la geografía española. El presente trabajo plantea un modelo que tiene como objetivo analizar la incidencia de las economías externas en la dis- tribución territorial del empleo de los distintos sectores industriales. El modelo incorpora

ELISABET VILADECANS MARSAL

2003-01-01

189

Boletín del Instituto Nacional del Cáncer - 27 de marzo de 2012  

Cancer.gov

Le invitamos a que comparta esta publicación con sus amigos, familiares y colegas y nos ayude a continuar nuestra misión de difundir las investigaciones más recientes sobre la prevención, el tratamiento y la información del cáncer.

190

Els títols dels films de Quentin Tarantino : una perspectiva multilingüe  

Microsoft Academic Search

Els títols de films en llengua anglesa són, avui dia, textos (o paratextos) de gran importància cultural en les societats occidentals. En aquest article ens ocupem de dues qüestions: (1) del fenomen —força popular— de la traducció dels títols de pel·lícules en llengua anglesa que arriben al nostres cinemes; repassem les variades tipologies emprades per analitzar les traduccions dels títols

José Santaemilia; Betlem Soler

2010-01-01

191

Análisis del comportamiento estructural de la basílica del Pilar de Zaragoza  

Microsoft Academic Search

En el artículo se presentan los trabajos de desarrollo y validación de un conjunto de modelos estructurales\\u000ade la basílica del Pilar de Zaragoza planteados con el objetivo de aproximar el estado estructural actual del\\u000atemplo y su nivel de seguridad. Los modelos numéricos se han formulado aplicando el MEF en teoría lineal\\u000ay no lineal, incluyendo el proceso constructivo

Luis E. Romera; Santiago Hernández; José Manuel Reinosa

1970-01-01

192

Boletín del Instituto Nacional del Cáncer - 31 de enero de 2012  

Cancer.gov

Dr. Nils Daulaire Salud mundial a través de la colaboración y el liderazgo Para conmemorar el Día Mundial del Cáncer que tuvo lugar el 4 febrero, el doctor Nils Daulaire, director de la Oficina de Asuntos Internacionales del Departamento de Salud y Servicios Humanos de los Estados Unidos habla sobre las razones por las cuales los Estados Unidos deben participar en iniciativas para mejorar la salud mundial.

193

Estudio para la prevención del cáncer necesita voluntarias: El Estudio del Tamoxifeno y Raloxifeno (STAR) se realiza en Norteamérica  

Cancer.gov

El Estudio del Tamoxifeno y Raloxifeno (STAR, por sus siglas en inglés), uno de los estudios más importantes realizados para la prevención del cáncer del seno, está ahora reclutando voluntarias en más de 400 centros en los Estados Unidos, Puerto Rico y Canadá.

194

Influence of cooling rate on the ability of frozen-thawed sperm to bind to heterologous zona pellucida, as assessed by competitive in vitro binding assays in the ocelot (Leopardus pardalis) and tigrina (Leopardus tigrinus).  

PubMed

We evaluated the influence of two cooling rates (from 25 to 5 degrees C) on post-thaw function of frozen sperm in ocelots (Leopardus pardalis; n=3 males) and tigrinas (Leopardus tigrinus; n=4 males). Seven normospermic (>70% normal sperm) electroejaculates from each species were diluted with a 4% glycerol freezing medium, divided into two aliquots, and assigned to one of two cooling rates: fast or slow (0.7 or 0.16 degrees C/min, respectively). Sperm motility index (SMI) and percentage of sperm with an intact acrosome were assessed before freezing and after thawing, and the ability of sperm to bind to the zona pellucida of IVM domestic cat oocytes were assessed in a competitive in vitro sperm-binding assay. Regardless of the cooling rate, frozen-thawed sperm from both species exhibited a SMI of 50; approximately 20 and approximately 32% of post-thaw sperm had an intact acrosome in ocelots and tigrinas, respectively (P<0.05). The mean (+/-S.E.M.) number of sperm bound per oocyte was higher for fast-cooled (8.5+/-1.3) than slow-cooled (2.5+/-0.3; P<0.01) ocelot sperm. In contrast, more tigrina sperm bound to domestic cat oocytes when cooled slowly versus quickly (5.8+/-0.9 versus 2.7+/-0.4, P<0.05). In conclusion, cryopreservation decreased sperm function in both species, and the oocyte-binding assay was the most efficient method to detect functional differences in post-thaw sperm. PMID:17977588

Baudi, D L K; Jewgenow, K; Pukazhenthi, B S; Spercoski, K M; Santos, A S; Reghelin, A L S; Candido, M V; Javorouski, M L; Müller, G; Morais, R N

2007-10-30

195

Evaporación dinámica del cinturón de asteroides original  

NASA Astrophysics Data System (ADS)

Hemos integrado numéricamente un enjambre de 3000 partículas en la región del cinturón de asteroides, sujetas a las perturbaciones gravitatorias de Júpiter y Saturno. Al cabo de 107 años, se han formado ya todos los gaps de Kirkwood observados en el cinturón actual. El grupo en la resonancia 3/2 es, aunque en menor grado, también visible. Los tiempos de Lyapunov computados para los asteroides sobrevivientes, nos ha permitido encontrar la fracción de ellos que es capaz de sobrevivir por toda la edad del sistema solar, merced a la aplicacion de la relación empírica que vincula dichos tiempos al tiempo en el cual se manifiestan grandes transiciones orbitales. La fracción de supervivientes es de aproximadamente 1/100. El resto de la masa faltante en el cinturón, es atribuible a colisiones catastróficas con cometas en etapas primordiales.

Brunini, A.

196

LA HABITABILIDAD DESDE UNA PERSPECTIVA SUBJETIVA: EL CASO DE LA AGEB 010-9 DEL FRACCIONAMIENTO OJO DE AGUA, MUNICIPIO DE TECÁMAC, ESTADO DE MÉXICO  

Microsoft Academic Search

El crecimiento desmedido e incontenible de la Zona Metropolitana de la Ciudad de México en los últimos 50 años, concatenado a las políticas de liberalización y desregulación económicas ha conformado un espacio con grandes núcleos de concentración generando problemas de habitabilidad y calidad de vida para sus habitantes. El diagnosticar la habitabilidad de dichas zonas es un trabajo complejo, pues

Osvaldo Velázquez Mejía

2010-01-01

197

El valor de uso recreativo del Parque Natural de L'Albufera a través del método indirecto del coste de viaje  

Microsoft Academic Search

La valoración económica de bienes que carecen de mercado, como son los espacios naturales protegidos, proporcionan una valiosa información que puede ser utilizada en el análisis coste-beneficio como fundamento de las decisiones públicas que afectan a la calidad del medio ambiente. En este trabajo se aplica el método indirecto del coste de viaje individual para obtener el valor de uso

S. DEL SAZ SALAZAR; L. PÉREZ Y PÉREZ

1999-01-01

198

Reavivando las Zonas Muertas de las Costas  

NSDL National Science Digital Library

The issue-focused, peer-reviewed article addresses how dead zones, or areas that cannot support life, are increasing along the coast due to: agricultural runoff and fertilizers industrial waste, and acid rain, which is produced by the burning of fossil fuels.

Robert Howarth (Cornell University;)

2000-09-01

199

Pluralisme raisonné dans un zona panorama  

Microsoft Academic Search

A classical observation of homeopathic treatment for intercostal herpes zoster has a particular rich symptomatology which well demonstrates the interest in a pluralistic reasoned prescription permitting the return to the original sensitive medicine of the patient, of which the prescription, after a classical, telltale pathogenic aggravation phase, permits the achievement of an effective cure.

Pierre Sirieix

2010-01-01

200

A Description of Del Mod and Its Final Evaluation, Final Report, Volume I.  

ERIC Educational Resources Information Center

This monograph presents an overview of five Del Mod System final reports, comments by the project director, financial structure of the Del Mod System, and descriptions of Del Mod Projects. The Del Mod System was concerned with changing the science and mathematics education programs in the state of Delaware. Between 1970 and 1976, Del Mod conducted…

Purnell, Charlotte H.; Bolig, John R.

201

An evening at "La Clinica del Pueblo".  

PubMed

This article describes a typical evening at the Clinica del Pueblo in the Hispanic neighborhood of Adams-Morgan in Washington, D.c. The Clinical del Pueblo began operating in 1983 in response to the urgen medical needs of Central American refugees arriving in the Washington D.c. area. The refugees bring with them severe trauma, fear, and health problems caused by the civil was and exacerbated by inadequate or non-existant health services. Approximately 80,000 Salvadoran refugees live in the area. They do not receive adequate health care for 3 reasons. 1) Because the US goverment is unwilling to recognize them as true refugees, they live with the constant threat of deportatin back to the violence from which they have fled. 2) Refugees lack the ability to pay for private care. 3) Langauage and culture create frightening barriers to health care for the refugees. For those who do seek care, these barriers can lead to the inadequate or incomplete diagnoses and poor compliance and follow-up. Plenty International and the Central American Refugee Center responded to these problems by organizing a free clinic to provide not only medical care but also a training course for volunteers. The director of the clinic organizes the course, the classes are taught by a variety of people including the clinic's volunteer physicians, nurses, and public health educators as well as graduates of previus training courses and people from the wider community. The services of the clinic reach only a small portion of the population in need. However, the fact that free medical services are now available to some Central American refugees make the Clinica del Pueblo an important program. PMID:12281149

Shefsky, M L

1986-01-01

202

UBVJHKLM photometry of Nova Del 2013  

NASA Astrophysics Data System (ADS)

We present the results of the optical and IR photometry of Nova Del 2013 obtained at the Crimean Station (Nauchny, Ukraine) of the Sternberg Astronomical Institute. UBV photometry was carried out with 60-cm Zeiss telescope using a photoelectric photometer on August 15.94 UT: U=4.54+/-0.02, B=5.07+/-0.01, V=5.05+/-0.01; on August 16.80 UT: U=4.54+/-0.01, B=4.85+/-0.02, V=4.68+/-0.01; on August 16.86 UT: U=4.66+/-0.01, B=4.92+/-0.01, V=4.73+/-0.01.

Burlak, A. M.; Shenavrin, I. V.; Tatarnikov, M. A.; Tatarnikova, A. A.

2013-08-01

203

Near Infrared Photometry of Nova Del 2013  

NASA Astrophysics Data System (ADS)

Subjects: Infra-Red, Nova We report RIJHKLM photometry of Nova Del 2013 taken on August 28.13 UT using an As:Si bolometer mounted on the 0.76-m infrared telescope of the University of Minnesota's O'Brien Observatory (Marine on St. Croix, Minnesota, USA). Vega (alpha Lyrae) was used as the standard star. On this date we find: R = 5.6 +/- 0.1, I = 5.2 +/- 0.1, J = 4.5 +/- 0.1, H = 4.7 +/- 0.1, K = 4.7 +/- 0.1, L = 3.3 +/- 0.2, M = +1.8 +/- 0.3.

Cass, C. A.; Carlon, L. R.; Corgan, T. D.; Dykhoff, A. D.; Gehrz, D. R.; Shenoy, P. D.

2013-08-01

204

The paleontological site in the Sierra del Chaparral (Villaluenga del Rosario, Cádiz-Spain)  

Microsoft Academic Search

This article outlines the results obtained during the first study of the Chaparral site (Villaluenga del Rosario, Cádiz, Spain) situated in the karst area of the Sierra de Grazalema. The varied macro- and micro-vertebrate fauna include carnivore, herbivores and rodents, as well as reptiles and amphibians, suggesting an Early Pleistocene age. The taphonomic study of the fauna discovered on the

Francisco Giles Pacheco; Antonio Santiago; José María Gutiérrez; Juan Manuel López-García; Hugues-Alexandre Blain; Gloria Cuenca-Bescós; Jan van der Made; Isabel Cáceres; Nuria García

205

MÚSICA Y AGRUPACIONES DE CARNAVAL A FINALES DEL SIGLO XX EN ANDALUCÍA OCCIDENTAL. LA REVITALIZACIÓN FESTIVA Y EL RENACIMIENTO DEL CARNAVAL  

Microsoft Academic Search

Con este trabajo pretendemos acercarnos al Carnaval de Andalucía occidental a partir de su recuperación durante la Transición y los gobiernos socialistas en la década de los años ochenta del pasado siglo XX. Este proceso tuvo lugar en un marco de participación ciudadana, modernización económica, estado del bienestar, constitución de las comunidades autónomas, sociedad del espectáculo y del entretenimiento, y

Francisco José García Gallardo

2005-01-01

206

Un mito del hambre en el nordeste de Brasil  

Microsoft Academic Search

El objetivo del presente estudio es permitir la comprensión, mediante un abordaje etnográfico, de los significados del hambre en las vivencias de actores sociales que enfrentan la pobreza extrema en un barrio de Salvador, Bahía, Brasil. Los relatos se han analizado con el objeto de entender la construcción de signos inherentes al sufrimiento de quienes padecen de hambre cotidianamente. En

Maria do Carmo S. Freitas; Paulo G. L. Pena

2003-01-01

207

ETNOGRAFÍA DEL GASTO: EL CASO DE UNA EMPRESA TURÍSTICA  

Microsoft Academic Search

El siguiente trabajo (a diferencia de otros) intenta ser un abordaje innovador sobre el tema que denota la etno-psicología del gasto (erogación). Asimismo, su presentación (en forma de comentarios etnográficos o cuaderno de notas) es combinada con datos estadísticos de gran relevancia y comentarios personales del observador durante el trabajo de campo. En este caso, se ha combinado prácticas propias

Maximiliano Korstanje

2008-01-01

208

Del Pezzo surfaces and affine 7-brane backgrounds  

Microsoft Academic Search

A map between string junctions in the affine 7-brane backgrounds and vector bundles on Del Pezzo surfaces is constructed using mirror symmetry. It is shown that the lattice of string junctions with support on an affine 7-brane configuration is isomorphic to the K-theory group of the corresponding Del Pezzo surface. This isomorphism allows us to construct a map between the

Tamás Hauer; Amer Iqbal

2000-01-01

209

The hydrothermal system of Nevado del Ruiz volcano, Colombia  

Microsoft Academic Search

Hot springs and steam vents on the slopes of Nevado del Ruiz volcano provide evidence regarding the nature of hydrothermal activity within the summit and flanks of the volcano. At elevations below 3000 m, alkali-chloride water is discharged from two groups of boiling springs and several isolated warm springs on the western slope of Nevado del Ruiz. Chemical and isotopic

Neil C. Sturchio; Stanley N. Williams; Nestor P. Garcia; Adela C. Londono

1988-01-01

210

confirmation of no X-rays from Nova Del 2013  

NASA Astrophysics Data System (ADS)

Due to an underestimation of the X-ray background for the particular Swift observation of Nova Del 2013 reported by us in ATel #5314, we agree with the non-detection given on ATel #5318 and determine a 3-sigma upper limit to the Nova Del 2013 count rate of 0.013 counts/sec. We apologize for the confusion.

Castro-Tirado, A. J.; Martin-Carrllo, A.; Hanlon, L.

2013-08-01

211

EL USO DEL ADRIAMICIN:UN PROBLEMA SIN SOLUCIÓN AÚN  

Microsoft Academic Search

aunque ésta se halla limitada por su cardiotoxicidad. Para este trabajo, estudio piloto diseñado aunque ésta se halla limitada por su cardiotoxicidad. Para este trabajo, estudio piloto diseñado aunque ésta se halla limitada por su cardiotoxicidad. Para este trabajo, estudio piloto diseñado para determinar si los indicadores del estrés oxidativo pudieran ser marcadores tempranos para determinar si los indicadores del

Belkis Martínez Sánchez; Rubén Castillo Jiménez

212

33 CFR 110.111 - Marina del Rey Harbor, Calif.  

Code of Federal Regulations, 2010 CFR

...Waters 1 2010-07-01 2010-07-01 false Marina del Rey Harbor, Calif. 110.111 Section 110.111 Navigation...REGULATIONS Special Anchorage Areas § 110.111 Marina del Rey Harbor, Calif. An area in the main channel within the...

2010-07-01

213

33 CFR 110.111 - Marina del Rey Harbor, Calif.  

Code of Federal Regulations, 2010 CFR

...Waters 1 2009-07-01 2009-07-01 false Marina del Rey Harbor, Calif. 110.111 Section 110.111 Navigation...REGULATIONS Special Anchorage Areas § 110.111 Marina del Rey Harbor, Calif. An area in the main channel within the...

2009-07-01

214

Orígen del deterioro del medio ambiente en Taiwan: Desajuste entre el modelo de desarrollo y la dotación de recursos naturales  

Microsoft Academic Search

De igual modo que el análisis de los llamados nuevos países industriales asiáticos (NPIAs) abre interesantes matices en el campo de las teorías del desarrollo, poniendo en duda la capacidad explicativa de los enfoques tanto dependentista como neoclásico, el estudio del medio ambiente en esos países aporta visiones nuevas y enriquecidas en lo referente a sus modelos de desarrollo. En

Antonio Rodríguez Carmona

1994-01-01

215

Valoración Económica y Ambiental Aplicada a Casos del Manejo de la Calidad del Aire y Control de la Contaminación  

Microsoft Academic Search

Este documento fue comisionado por la Red de Medio Ambiente del Diálogo Regional de Política para la III Reunión Hemisférica celebrada los días 9 y 10 de marzo de 2004. Durante las últimas décadas se han establecido derechos y obligaciones ciudadanas; se han definido las funciones del Estado y de los organismos públicos responsables en materia ambiental; se han realizado

Luis A. Cifuentes; Luis Rizzi; Héctor Jorquera; Javier Vergara

2004-01-01

216

Carrier frequency of GJB2 gene mutations c.35delG, c.235delC and c.167delT among the populations of Eurasia.  

PubMed

Hearing impairment is one of the most common disorders of sensorineural function and the incidence of profound prelingual deafness is about 1 per 1000 at birth. GJB2 gene mutations make the largest contribution to hereditary hearing impairment. The spectrum and prevalence of some GJB2 mutations are known to be dependent on the ethnic origin of the population. This study presents data on the carrier frequencies of major GJB2 mutations, c.35delG, c.167delT and c.235delC, among 2308 healthy persons from 18 various populations of Eurasia: Russians, Bashkirs, Tatars, Chuvashes, Udmurts, Komi-Permyaks and Mordvins (Volga-Ural region of Russia); Belarusians and Ukrainians (East Europe); Abkhazians, Avars, Cherkessians and Ingushes (Caucasus); Kazakhs, Uighurs and Uzbeks (Central Asia); and Yakuts and Altaians (Siberia). The data on c.35delG and c.235delC mutation prevalence in the studied ethnic groups can be used to investigate the prospective founder effect in the origin and prevalence of these mutations in Eurasia and consequently in populations around the world. PMID:20739944

Dzhemileva, Lilya U; Barashkov, Nikolay A; Posukh, Olga L; Khusainova, Rita I; Akhmetova, Vita L; Kutuev, Ildus A; Gilyazova, Irina R; Tadinova, Vera N; Fedorova, Sardana A; Khidiyatova, Irina M; Lobov, Simeon L; Khusnutdinova, Elza K

2010-08-26

217

El metabolismo social del País Vasco desde el análisis de flujos materiales  

Microsoft Academic Search

La desvinculación de la mejora del bienestar respecto del uso de los recursos y de la contaminación es esencial para lograr un desarrollo sostenible. Para avanzar en esta dirección es preciso partir del conocimiento del metabolismo de la sociedad. Es decir, debemos analizar en qué medida nuestras sociedades “ingieren” materias primas, que son “metabolizadas” para producir bienes y servicios, y

Iñaki Arto

2009-01-01

218

La teoría del desarrollo desigual de Oscar Braun. Un análisis crítico  

Microsoft Academic Search

En este análisis crítico de la teoría del intercambio desigual de Oscar Braun, intentamos movernos consistentemente a través de un número de etapas analíticas distintas. Estas etapas son: a) una discusión sobre el objeto del conocimiento en Braun, sin las restricciones de su propia problemática (sección 3); b) una confrontación del objeto del conocimiento en Braun con el objeto real

Richard Brown; Philip Wright

1979-01-01

219

88. Paso Del Norte Hotel, 115117 South El Paso St., ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

88. Paso Del Norte Hotel, 115-117 South El Paso St., south facade, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

220

Current Work in Laser Cleaning of the Porta del Paradiso  

NASA Astrophysics Data System (ADS)

This paper summarises the conservation of the Porta del Paradiso, currently underway. It briefly illustrates the two cleaning methods employed and the reasons leading to continue the cleaning of the perimeter panels with laser ablation, describing the various working phases.

Agnoletti, S.; Brini, A.; Nicolai, L.

221

Proposed Bosque Del Apache Wilderness Area, New Mexico.  

National Technical Information Service (NTIS)

The report describes the proposal which recommends that approximately 32,500 acres within the Bosque del Apache National Wildlife Refuge in Socorro County, New Mexico, be designated as wilderness within the National Wilderness Preservation System. Since d...

1972-01-01

222

Del Bueno Recalls Cheese Because Of Possible Health Risk  

NASA Website

Del Bueno of Grandview, WA is recalling all size packages of Queso Fresco Fresh Cheese, Queso Panela Fresh Cheese, Requeson Mexican Style Ricotta Cheese, and Queso Enchilado Dry Cheese because they have the potential to be contaminated with Listeria ...

223

Extreme Environments in Tierra del Fuego, Argentina  

NASA Astrophysics Data System (ADS)

The upper timberline of the Andes Cordillera on the island of Tierra del Fuego at the tip of South America is an environment subject to extreme conditions. In order to further understand this environment, ecosystem parameters were measured within two transects of the Andes at Glaciar Martial and Cerro Guanaco. The measurements included pH, soil temperature, soil moisture, nitrogen, sodium and potassium concentration, chlorophyll absorbance, and irradiance in the ultraviolet range (200-400 nm). These data comprise a survey that serves as a baseline for an intensive research program. Chlorophyll concentration and soil data were within the range of our observations at several other sites, from Lapataia Bay on the southwestern boundary with Chile, through the eastern end of Lake Fagnano. However, unusual levels of solar irradiance were found in the open sites of both transects while those in the forest exhibited lower UV values, suggesting strong absorption and/or reflection by the forest canopy. High levels of UV radiation damage important biomolecules and may be partially responsible for the presence of life forms such as the krummholz belt in the upper timberline. These UV values may be due to the effects of global ozone depletion and the ozone hole. The low temperatures, strong winds, snow and ice-covered soil and especially the exposure to UV radiation make this area an extreme environment for life.

Schultz, C.; D'Antoni, H.; Burgess, S.; Zamora, J.; Skiles, J.

2007-12-01

224

Antenatal Rh prophylaxis is unnecessary for “Asia type” DEL women  

Microsoft Academic Search

BackgroundGenerally Rh-negative patients need to be transfused with Rh-negative red blood cells. For pregnant women carrying Rh-positive fetus, the antenatal anti-D detection and Rh immunoglobulin prophylaxis are required worldwide. In East Asia, a RhD variant, termed “Asia type” DEL, was found in approximately 30% of apparent Rh-negative individuals. The antigenic and molecular properties of the DEL were previously defined. Few

Chao-Peng Shao; Hua Xu; Qun Xu; Guo-Dong Sun; Jian-Ping Li; Bo-Wei Zhang; Xiao-Hua Liang; Zhong Liu; Ying Zhou; Dan Li; Nai-Bao Zhuang

2010-01-01

225

Los cambios comportamentales y del uso del espacio asociados a la muerte del macho dominante de una manada de lobos ibéricos (canis lupus signatus) en el Parque Zoológico de Barcelona  

Microsoft Academic Search

La dinámica social es uno de los campos más estudiados de la familia Canidae en condiciones de cautividad. En este estudio se pretende determinar cómo la muerte del macho dominante de una manada de lobos alojados en el Parque Zoológico de Barcelona afecta al patrón de actividad diario y al uso del espacio del resto de coespecíficos. Los individuos objeto

Ana Isabel Soriano Giménez; Susana Serrat Navarro; Conrad Ensenyat; Carles Riba; Carmen Maté García

2006-01-01

226

Difusión de resultados iniciales del Estudio del Tamoxifeno y del Raloxifeno (STAR): el fármaco para el tratamiento de la osteoporosis, raloxifeno, es tan efectivo como el Tamoxifeno en la prevención de cáncer de seno invasor  

Cancer.gov

Los resultados iniciales del Estudio del Tamoxifeno y del Raloxifeno, STAR, demuestran que el fármaco raloxifeno, actualmente utilizado para prevenir y tratar la osteoporosis en mujeres posmenopáusicas, funciona tan bien como el tamoxifeno para reducir el riesgo de cáncer de seno entre las mujeres posmenopáusicas que tienen riesgo elevado de padecer la enfermedad. Preguntas y respuestas

227

Ongoing near-infrared observations of V339 Del (Nova Del 2013)  

NASA Astrophysics Data System (ADS)

We report continuing 1.08 to 2.35 micron near-infrared spectroscopy of Nova Del 2013 in the J,H,K bands with the Mount Abu 1.2 meter telescope (+ PRL Near-Infrared NICMOS3 Imager/Spectrograph). Subsequent to our initial report on August 28.674 UT (ATel #5337), spectra of the nova have been obtained at regular intervals. The latest spectra of September 16.5 and 19.5 UT are dominated by strong emission lines but the earlier reported strengths of many of the lines have changed significantly.

Banerjee, D. P. K.; Ashok, N. M.; Joshi, Vishal; Evans, Nye

2013-09-01

228

A Case of Near-triploidy in Myelodysplastic Syndrome with del(5q) Combined with del(1p) and del(13q)  

PubMed Central

Numerical and structural chromosomal abnormalities are common in hematological malignancies. Near-triploidy (58-80 chromosomes) is a numerical abnormality observed in 3% of adult cases of acute lymphoblastic leukemia. Near-triploidy is rare in myeloid lineage hematologic malignancies and compared to near-triploidy in lymphoid malignancies, near-triploidy in myeloid malignancies is associated with poor outcomes. Few studies on near-triploidy in myelodysplastic syndrome (MDS) have been reported, and the clinicopathologic significance of this condition is still unclear. Here, we report a novel case of MDS with near-triploidy and multiple structural chromosomal abnormalities: del(5q) combined with del(1p) and del(13q). These abnormalities were detected by cytogenetic analysis with array comparative genomic hybridization (CGH). Our results suggest that array CGH can be a useful tool for detecting chromosomal abnormalities in patients with MDS.

Kim, Bo-Ram; Kim, Ji-Eun; Woo, Kwang-Sook; Kim, Kyeong-Hee; Kim, Jeong-Man; Lee, Suee; Shaffer, Lisa G.

2012-01-01

229

V339 Del (Nova Del 2013) is a weak non-super-soft X-ray source  

NASA Astrophysics Data System (ADS)

V339 Del (Nova Del 2013) is currently being monitored approximately daily by Swift-XRT. X-ray analysis has been complicated due to optical loading caused by photons from the very bright optical source. (For a discussion of optical loading in the XRT, see our help page.) At early times, the data were collected mainly in Windowed Timing mode to mitigate this issue, and no X-ray source was detected (ATel #5283, #5305, #5318).

Page, K. L.; Beardmore, A. P.

2013-09-01

230

La reparación del daño como mecanismo de tutela de la persona. Del daño a la salud a los nuevos daños extrapatrimoniales  

Microsoft Academic Search

En la actualidad, el operador del Derecho se enfrenta a dos de los más importantes problemas del nuevo derecho de daños: por un lado, la clasificación del perjuicio y, por el otro, la reparación correspondiente a cada una de sus categorías, que debe estar informada por el principio de reparación integral, y que impone en consecuencia considerar más que las

Milagros Koteich Khatib

231

Estudio multifrecuencia del medio interestelar cercano a HD 192281  

NASA Astrophysics Data System (ADS)

Una de las causas que modifica la estructura y dinámica del medio interestelar es la acción que los vientos de las estrellas de gran masa ejercen sobre el mismo. En este trabajo, mediante el uso de datos interferométricos obtenidos en la banda de radio en la transición de ?˜21-cm del hidrógeno neutro y de imágenes de la emisión de continuo en las bandas de 408 y 1420 MHz, de imágenes HIRES del satélite IRAS en 60 y 100?m, y de observaciones de continuo obtenidas con radiotelescopios de disco simple en 2695, 4850 y 8350 MHz se ha realizado un estudio multifrecuencia de los efectos que los vientos estelares de HD 192281, una estrella de tipo espectral O5,Vn((f))p, han tenido sobre el medio interestelar que rodea a la misma.

Arnal, E. M.; Cappa, C. E.; Cichowolski, S.; Pineault, S.; St-Louis, N.

232

Estudio multifrecuencia del medio interestelar cercano a HD 192281  

NASA Astrophysics Data System (ADS)

Una de las causas que modifica la estructura y dinámica del medio interestelar es la acción que los vientos de las estrellas de gran masa ejercen sobre el mismo. En este trabajo, mediante el uso de datos interferométricos obtenidos en la banda de radio en la transición de 21-cm del Hidrógeno neutro y de imágenes de la emisión de continuo en las bandas de 408 y 1420 MHz, de imágenes HIRES del satélite IRAS en 60 y 100 micrones, y de observaciones de continuo obtenidas con radiotelescopios de disco simple en 2695, 4850 y 8350 MHz se ha realizado un estudio multifrecuencia de los efectos que los vientos estelares de HD 192281, una estrella de tipo espectral O5 Vn((f))p, han tenido sobre el medio interestelar que rodea a la misma.

Arnal, E. M.; Cappa, C.; Cichowolski, S.; Pineault, S.; St-Louis, N.

233

Categories of massless D-branes and del Pezzo surfaces  

NASA Astrophysics Data System (ADS)

In analogy with the physical concept of a massless D-brane, we define a notion of "" for objects in the derived category. This is defined in terms of monodromy around singularities in the stringy Kähler moduli space and is relatively easy to study using "spherical functors". We consider several examples in which del Pezzo surfaces and other rational surfaces in Calabi-Yau threefolds are contracted. For precisely the del Pezzo surfaces that can be written as hypersurfaces in weighted , the category of objects is a "fractional Calabi-Yau" category of graded matrix factorizations.

Addington, Nicolas; Aspinwall, Paul S.

2013-07-01

234

Continuing optical spectroscopy of V339 Del = Nova Del 2013 with the Nordic Optical Telescope and the ARAS Group  

NASA Astrophysics Data System (ADS)

Since our first report (ATel#5312) we have been continuing nightly, almost hourly spectroscopic observations of V339 Del = Nova Del 2013 = PNV J20233073+2046041). Here we report the state at approximately the t_3 point in the photometric decline (see also ATel#5370). Spectra have been obtained with the 2.6 m Nordic Optical Telecope FIbre-fed Echelle Spectrograph (FIES) (R ~ 67000), the Ondrejov Observatory 2m Zeiss coude spectrograph (R = 18000), and a variety of grating and echelle spectrographs of the ARAS group with resolutions ranging from 580 - 11000.

Shore, S. N.; Alton, K.; Antao, D.; Barbotin, E.; Berardi, P.; Bohlsen, P.; Boubault, F.; Boyd, D.; Briol, J.; Buil, C.; Charbonnel, S.; Dubreuil, P.; Dubs, M.; Edlin, J.; de France, T.; Favaro, A.; Garde, O.; Graham, K.; Greenan, D.; Guarro, J.; Hansen, T.; Hyde, D.; Lemoult, T.; Leadbeater, R.; Martineau, G.; Buchet, Y.; Masviel, J. P.; Montier, J.; Pollmann, E.; Ribeiro, J.; Thizy, O.; Terry, J.-N.; Teyssier, F.

2013-09-01

235

First high resolution ultraviolet (HST/STIS) and supporting optical spectroscopy of V339 Del = Nova Del 2013  

NASA Astrophysics Data System (ADS)

V339 Del = Nova Del 2013 was observed with the Space Telescope Imagine Spectrograph (STIS) aboard the Hubble Space Telescope (HST) on 2013 Sept. 18.2 UT from 1140-2888 A using the E140M and G230H gratings at a resolution of ~ 100000. At the time, V ~ 8, shortly after t_3. Continuing spectroscopy was provided by the Astronomical Ring for Access to Spectroscopy (ARAS) at resolutions ranging from 3684 - 7431 A with resolutions ranging from 580 - 11000 with time sequences as short as 10 minutes at resolutions up to 12000.

Shore, S. N.; Schwarz, G. J.; Alton, K.; Antao, D.; Barbotin, E.; Berardi, P.; Blank, T.; Bohlsen, T.; Boubault, F.; Boyd, D.; Briol, J.; Buil, C.; Charbonnel, S.; Dubreuil, P.; Dubs, M.; Edlin, J.; de France, T.; Favaro, A.; Garde, O.; Graham, K.; Greenan, D.; Guarro, J.; Hansen, T.; Hyde, D.; Lemoult, T.; Leadbeater, R.; Martineau, G.; Buchet, Y.; Masviel, J. P.; Montier, J.; Mauclaire, B.; Pollmann, E.; Ribeiro, J.; Schram, B.; Thizy, O.; Terry, J.-N.; Teyssier, F.; Page, K. L.; Osborne, J. P.; Ness, J.-U.; Starrfield, S.; Walter, F. M.; Woodward, C. E.; Bode, M. F.

2013-09-01

236

The novel c.247_249delTTC (p.F83del) GJB2 mutation in a family with prelingual sensorineural deafness.  

PubMed

Non-syndromic hearing loss is one of the most common hereditary determined diseases in human, and the disease is a genetically heterogeneous disorder. Mutations in the GJB2 gene, encoding connexin 26 (Cx26), are a major cause of non-syndromic recessive hearing impairment in many countries and are largely dependent on ethnic groups. Due to the high frequency of the c.35delG GJB2 mutation in the Greek population, we have previously suggested that Greek patients with sensorineural, non-syndromic deafness should be tested for the c.35delG mutation and the coding region of the GJB2 gene should be sequenced in c.35delG heterozygotes. Here we present on the clinical and molecular genetic evaluation of a family suffering from prelingual, sensorineural, non-syndromic deafness. A novel c.247_249delTTC (p.F83del) GJB2 mutation was detected in compound heterozygosity with the c.35delG GJB2 mutation in the proband and was later confirmed in the father, while the mother was homozygous for the c.35delG GJB2 mutation. We conclude that compound heterozygosity of the novel c.247_249delTTC (p.F83del) and the c.35delG mutations in the GJB2 gene was the cause of deafness in the proband and his father. PMID:22484064

Petersen, Michael B; Grigoriadou, Maria; Koutroumpe, Maria; Kokotas, Haris

2012-04-06

237

Screening of 185DelAG, 1014DelGT and 3889DelAG BRCA1 mutations in breast cancer patients from North-East India.  

PubMed

Around 1.35 million people of worldwide suffer from breast cancer each year, whereas in India, 1 in every 17 women develops the disease. Mutations of the Breast Cancer 1 (BRCA1) gene account for the majority of breast/ ovarian cancer families. The purpose of study was to provide a prevalence of BRCA1 germline mutations in the North-East Indian population. In relation to the personal and family history with the breast cancer, we found mutations in 6.25% and 12.5% respectively. Three mutations, 185DelAG, 1014DelGT and 3889DelAG, were observed in our North-East Indian patients in exons 2 and 11, resulting in truncation of the BRCA1 protein by forming stop codons individually at amino acid positions 39, 303 and 1265. Our results point to a necessity for an extensive mutation screening study of high risk breast cancer cases in our North-East Indian population, which will provide better decisive medical and surgical preventive options. PMID:23317271

Hansa, Jagadish; Kannan, Ravi; Ghosh, Sankar Kumar

2012-01-01

238

Contribució al coneixement dels Noctuidae de la Garrotxa (Catalunya) (Lepidoptera: Noctuidae)  

Microsoft Academic Search

L'objecte d'aquest treball és presentar una actualització del catàleg dels Noctuidae de la Garrotxa. Es donen els resultats de l'estudi del material arreplegat per l'autor durant els darrers deu anys i del dipositat en abres col.leccions, i s'inclouen, també totes les dades de la bibliografia existent. El cens de Noctuidae de la Garrotxa queda establert provisionalment en 309 especies. Es

Josep Bellavista i Admetlla

1999-01-01

239

Delia H. Etcheverry participó en 2008 del Seminario \\  

Microsoft Academic Search

El encaje es una técnica textil relativamente nueva; aparece en Europa en el siglo XVI y tiene su apogeo en los siglos XVII y XVIII. Sin embargo, cuando se menciona que un trabajo de encaje está hecho a mano, inmediatamente se lo asocia a una época mucho más anti­ gua, a piezas del pasado que se han quedado en el

Por Delia Etcheverry

240

Racionalidad limitada en la sociedad del riesgo mundial  

Microsoft Academic Search

Durante muchas décadas se ha pretendido que la teoría económica de la elección racional es la teoría general del comportamiento humano de la que carecen las ciencias sociales. Este artículo señala algunas de sus limitaciones. La psicología cognitiva describe los sesgos cognitivos de las personas cuando evalúan la información y eligen entre opciones, los cuales ponen en cuestión la idea

Francisco Augusto Laca Arocena

2012-01-01

241

Il ruolo del turismo per l'integrazione Europea  

Microsoft Academic Search

Nell'Unione Europea l'espansione del settore turi stico è destinato a svolgere un ruolo di grande rilievo, per molteplici motivi. Inn anzitutto il turismo ha un notevole impatto economico, in quanto contribuisce positivamente all a creazione di nuovi posti di lavoro e alla crescita di reddito. Inoltre può potenziare le politiche regionali e la politica agricola comune sostenendo lo sviluppo delle

Giulio Querini; Carmen Bizzarri

2009-01-01

242

Las Máscaras Del Héroe: Radiografía De Un Anti-Héroe  

Microsoft Academic Search

Las máscaras del héroe depicts Spanish society from the Second Republic to the start of the Civil War, a period when Spain was torn by poverty and immorality. This intertextual historical novel portrays figures such as Pedro Luis de Gálvez, the last of the bohemians and a failed writer. We encounter Gálvez through the first-person narration of Navales, who hates

Emilio Ramón

2009-01-01

243

The mercury clock of the Libros del Saber  

Microsoft Academic Search

The Libros del Saber de Astronomia is a compilation of various Arabic astronomical works translated into Castilian in the second half of the thirteenth century, under the direction of King Alfonso X of Spain. A section describing a mercury clock has been suggested to be of particular significance in view of the likely invention of the mechanical clock around this

A. A. Mills

1988-01-01

244

Medicine alternative: il senso del male nella postmodernità  

Microsoft Academic Search

Tra modernità e tradizione. E' palese la fortuna che le medicine cosiddette alternative o complementari stanno riscuotendo negli ultimi anni in Italia, come in molti altri Paesi occidentali. Esse interessano un pubblico sempre più ampio e differenziato, ed occupano ormai una fetta consistente dei servizi sanitari e del relativo mercato. Parallelamente, si diffondono in modo assai ampio certe concezioni culturali

Fabio Dei

245

87. Paso Del Norte Hotel, 115117 South El Paso St., ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

87. Paso Del Norte Hotel, 115-117 South El Paso St., south and east facades, west side of street - South El Paso Street Historic District, South El Paso, South Oregon & South Santa Fe Streets, El Paso, El Paso County, TX

246

¿RAZÕN DE AMOR O DENUESTOS DEL AGUA Y EL VINO?  

Microsoft Academic Search

Las dificultades que plantea para su clasificación genérica, su filiación lingüística y su interpretación estética hacen de la Razón de amor con los Denuestos del agua y el vino un apasionante enigma de la poesía medieval castellana; y el problema central en torno al cual parecen gravitar todas esas dificultades es la aparente dicotomía y falta de unidad de la

Arsenio Pacheco

1974-01-01

247

Assessing Glacier Hazards At Ghiacciaio Del Belvedere, Macugnaga, Italian Alps  

Microsoft Academic Search

The uppermost section of the Valle Anzasca behind and above the community of Macugnaga in the Italian Alps is one of the most spectacular high-mountain land- scapes in Europe, with gigantic rock walls and numerous steep hanging glaciers. Its main glacier, Ghiacciaio del Belvedere at the foot of the huge Monte Rosa east face, is a heavily debris-covered glacier flowing

W. Haeberli; M. Chiarle; G. Mortara; A. Mazza

2002-01-01

248

Interdisciplinary Unit: La Isla del Encanto (The Enchanted Island).  

ERIC Educational Resources Information Center

This document presents a series of 14 lesson plans in an interdisciplinary Spanish unit on "La isla del encanto/The Enchanted Island." The materials were prepared for students in grades 5 or 6 who have had basic Spanish instruction in previous grades. The students should also be familiar with basic concepts in English such as math computation, map…

Ford-Guerrera, Rebecca

249

Medical Ethnobotany of the Teribes of Bocas del Toro, Panama  

Microsoft Academic Search

Ethnomedical uses of 108 medicinal plant species, belonging to 52 families, 89 genera, used by the Teribe Amerindians of Bocas del Toro Province in Panama, along with their socio-cultural practices are reported here. The methods of administration of the herbal remedies, the plant parts used, their families and local names are also documented. The recorded medicinal plants were used mainly

M. P. Gupta; P. N. Solís; A. I. Calderón; F. Guinneau-Sinclair; M. Correa; C. Galdames; C. Guerra; A. Espinosa; G. I. Alvenda; G. Robles; R. Ocampo

2005-01-01

250

Interdisciplinary Unit: La Isla del Encanto (The Enchanted Island).  

ERIC Educational Resources Information Center

|This document presents a series of 14 lesson plans in an interdisciplinary Spanish unit on "La isla del encanto/The Enchanted Island." The materials were prepared for students in grades 5 or 6 who have had basic Spanish instruction in previous grades. The students should also be familiar with basic concepts in English such as math computation,…

Ford-Guerrera, Rebecca

251

Design, aerodynamics and autonomy of the DelFly.  

PubMed

One of the major challenges in robotics is to develop a fly-like robot that can autonomously fly around in unknown environments. In this paper, we discuss the current state of the DelFly project, in which we follow a top-down approach to ever smaller and more autonomous ornithopters. The presented findings concerning the design, aerodynamics and autonomy of the DelFly illustrate some of the properties of the top-down approach, which allows the identification and resolution of issues that also play a role at smaller scales. A parametric variation of the wing stiffener layout produced a 5% more power-efficient wing. An experimental aerodynamic investigation revealed that this could be associated with an improved stiffness of the wing, while further providing evidence of the vortex development during the flap cycle. The presented experiments resulted in an improvement in the generated lift, allowing the inclusion of a yaw rate gyro, pressure sensor and microcontroller onboard the DelFly. The autonomy of the DelFly is expanded by achieving (1) an improved turning logic to obtain better vision-based obstacle avoidance performance in environments with varying texture and (2) successful onboard height control based on the pressure sensor. PMID:22617112

de Croon, G C H E; Groen, M A; De Wagter, C; Remes, B; Ruijsink, R; van Oudheusden, B W

2012-05-22

252

Tratamiento del paludismo pediátrico: Papel de las intervenciones nutricionales  

Microsoft Academic Search

ResumenEl paludismo es una causa primordial de morbilidad y mortalidad en niños, particularmente en África subsahariana. Aunque se ha logrado un progreso considerable en el tratamiento del paludismo pediátrico con el tratamiento de combinación basado en la artemisinina, siguen existiendo numerosos obstáculos que se oponen a la puesta en práctica efectiva de estas nuevas opciones terapéuticas sumamente eficaces. Análogamente, si

Akoto Kwame Osei; Davidson H. Hamer

2008-01-01

253

DISTENSION ABDOMINAL Y EDEMAS POR QUISTE DEL CORDON ESPERMATICO  

Microsoft Academic Search

Resumen La distensión abdominal es un síntoma común, siendo en general la presentación inicial de enfer- medades sistémicas o desórdenes gastrointestinales. Otras causas son infrecuentes. Los quistes del cordón espermático son poco frecuentes, pero aún más su ubicación intraabdominal, su tamaño habitual es insuficiente para producir distensión. El paciente presentado en este caso es un varón con criptorquidia bilateral admitido

GUILLERMO A. KELLER; CECILIA SESSA

254

Historia del Tiempo Presente e Historia de las Relaciones Internacionales  

Microsoft Academic Search

Es la Historia del Presente una disciplina en tiempo de elaboración teórica. El rasgo específico de nuestro presente histórico bien pudiera la sociedad tecnológica en construcción. Puesto que la sociedad tecnológica nos introduce en un entorno desterritorializado con un protagonismo de la información sin precedentes en la historia, intuimos que nos adentramos en una sociedad internacional singular, propia exclusivamente de

Montserrat Huguet Santos

2001-01-01

255

Lo multicultural como mitología y como coartada del racismo  

Microsoft Academic Search

La noción de cultura en tanto que conjunto de caracteres de un colectivo nacional, tribal o religioso, ha venido a reemplazar por equivalencia la noción de raza, desprestigiada por la experiencia nazi. Pero su funcionamiento objetivo es el mismo. Raza, nación y cultura son constructos nacidos a la sombra del Romanticismo, de la reacción antiiluminista. El multiculturalismo es la ideología

Horacio Vázquez Rial

2007-01-01

256

Un Análisis del Testimonio en The Little School: Tales of Disappearance and Survival de Alicia Partnoy  

Microsoft Academic Search

El testimonio, como género en la literatura latinoamericana, es muy único. El punto de vista del autor es distinto ya que se deriva del sufrimiento vivido directamente por el testigo. Lo que hace especial al testimonio es la razón principal por la cual se escribe. El testimonio es un acto político que se basa en la fuerza del espíritu de

Jessica Verbanac

2009-01-01

257

TIPOLOGÍA DE CLASES Y SISTEMA DE EVALUACIÓN EN LA ENSEÑANZA DEL DERECHO EN CUBA  

Microsoft Academic Search

En la enseñanza del Derecho cobra vida un grupo de categorías didácticas, aplicadas al desarrollo sistémico del proceso docente-educativo, que constituyen la base donde se sostiene una parte importante de la teoría pedagógica. Entre estas categorías se encuentran las formas organizativas y la evaluación del aprendizaje, las que poseen un carácter universal, a partir de su expresión en los distintos

Carlos Justo Bruzón Viltres

2010-01-01

258

Síndrome del cuidador de adultos mayores discapacitados y sus implicaciones psicosociales  

Microsoft Academic Search

Objetivo: Describir la prevalencia del síndrome del cuidador y las características psicosociales de los cuidadores de adultos mayores discapacitados. Materiales y métodos: Se realizó un estudio piloto de casos y controles en el Valle del Cauca durante el 2003-2004 para evaluar la funcionalidad familiar (APGAR familiar), la ansiedad y depresión (escala de Goldberg), la presencia de enfermedades (cuidadores y no

ELIANA DUEÑAS; M ARÍA ANGGELINE MARTÍNEZ; B ENJAMÍN MORALES; CLAUDIA MUÑOZ; A NA SOFÍA VIÁFARA; J ULIÁN; A. HERRERA

2006-01-01

259

Fallas estructurales del mercado de maíz. Comentario a Robles y García  

Microsoft Academic Search

El artículo es un esfuerzo valioso por parte de economistas para poner (por fin) a funcionar el instrumental de la teoría económica sobre un tema del que sólo parecen ocuparse antropólogos y agrónomos. La carencia de investigación empírica y de reflexión analítica sobre aspectos centrales de la realidad económica del país habla mal del estado que guarda la disciplina de

Ángel Palerm

1995-01-01

260

El tutor del docente en formación, figura necesaria en la formación inicial del profesional de la educación  

Microsoft Academic Search

El desarrollo de la universalización pedagógica ha revitalizado el papel del tutor y actualmente cobra mayor importancia si queremos elevar la calidad en la formación y egreso de los futuros docentes. El tutor debe promover, facilitar, ayudar al docente en formación, creando las condiciones para su crecimiento personal y profesional, sin embargo, se ha detectado que es insuficiente su preparación

Maribel Asín Cala; María Isabel Jiménez Martínez; Imara Lay Bravo

2011-01-01

261

LA CONSOLIDACIÓN DEL HAMBRE Y DEL DERECHO A LA ALIMENTACIÓN EN LAS AGENDAS POLÍTICAS DE AMÉRICA LATINA  

Microsoft Academic Search

América Latina y el Caribe es la única región en desarrollo que puede acabar con el hambre en nuestra generación. Tiene 52,4 millones de personas subnutridas (10% de población) y 9 millones de niños con desnutrición crónica, pero también es la principal productora mundial de alimentos y tiene recursos financieros, naturales, humanos e institucionales para hacer del hambre un recuerdo

Jose Luis Viveros; Niemeyer Almeida Filho

2008-01-01

262

The leukocyte integrin antagonist Del-1 inhibits IL-17-mediated inflammatory bone loss  

PubMed Central

Aging is linked to increased susceptibility to chronic inflammatory diseases several of which, including periodontitis, involve neutrophil-mediated tissue injury. Here, we found that aging-associated periodontitis was accompanied by diminished expression of Del-1 (EDIL3), an endogenous inhibitor of LFA-1 integrin-dependent neutrophil adhesion, and by a reciprocal increase in IL-17 expression. Consistently, IL-17 inhibited gingival endothelial cell expression of Del-1, thereby promoting LFA-1-dependent neutrophil recruitment. Young Del-1-deficient mice developed spontaneous periodontitis featuring excessive neutrophil infiltration and IL-17 expression; disease was prevented in Del-1–LFA-1 and Del-1–IL-17 receptor double-deficient mice. Locally administered Del-1 inhibited IL-17 production, neutrophil accumulation, and bone loss. Therefore, Del-1 suppresses LFA-1-dependent neutrophil recruitment and IL-17-triggered inflammatory pathology and may thus be a promising therapeutic for inflammatory diseases.

Eskan, Mehmet A.; Jotwani, Ravi; Abe, Toshiharu; Chmelar, Jindrich; Lim, Jong-Hyung; Liang, Shuang; Ciero, Paul A.; Krauss, Jennifer L.; Li, Fenge; Rauner, Martina; Hofbauer, Lorenz C.; Choi, Eun Young; Chung, Kyoung-Jin; Hashim, Ahmed; Curtis, Michael A.; Chavakis, Triantafyllos; Hajishengallis, George

2012-01-01

263

FLUJOS DE COMERCIO INTRAINDUSTRIAL DE LAS EXPORTACIONES REPRESENTATIVAS ENTRE VALLE DEL CAUCA, ECUADOR Y VENEZUELA 1980 – 2006  

Microsoft Academic Search

En el presente trabajo se analiza el comportamiento del comercio intraindustrial (CII) de los subsectores representativos de la industria del Valle del Cauca con los países de Ecuador y Venezuela para el período 1980-2006 a través del índice Greenaway y Milner y el índice de Brülhart. De esta forma se corrige el problema de agregación presentado en mediciones anteriores y

Aura María Muñoz Cardona; Lina Johanna Salinas Luna

2009-01-01

264

A model of Nevado del Ruiz Volcano, Colombia  

NASA Astrophysics Data System (ADS)

A workshop was held at the Observatorio Vulcanologico de Colombia in Manizales, Colombia, March 28 to April 1, 1988, to review the accumulated data on the disastrous November 13, 1985, eruption and related activity of Nevado del Ruiz volcano (Figure 1). Approximately 30 scientists from eight countries presented data, developed a preliminary model, and addressed the future problems of Ruiz. The volcano (see Figure 2) erupted just prior to the meeting, covering the meeting site with 1-2 mm of ash.The workshop was convened by Stanley N. Williams (Louisiana State University, Baton Rouge) and Hansjürgen Meyer (Universidad del Valle, Cali, Colombia). A special volume of the Journal of Volcanology and Geothermal Research is planned for the papers and letters presented at the workshop, with some additional contributions. Publication is expected in mid-1989.

Williams, Stanley N.; Meyer, Hansjürgen

265

Recent Optical Spectroscopy of V339 Del 2013  

NASA Astrophysics Data System (ADS)

C. E. Woodward (U. Minnesota), R. M. Wagner (Ohio State U.), R. A. Hounsell (STScI), S. Starrfield (Arizona State U.) report optical spectroscopy of V339 Del obtained on on 2013 October 20.14 UT using the Steward Observatory 2.3 m Bok telescope on Kitt Peak with the B&C spectrograph covering the spectral range 325.0 to 925.0 nm at a spectral resolution of ~0.63 nm. In addition, we obtained an earlier spectrum of V339 Del on 2013 September 5.14 UT with the 2.4 m Hiltner telescope of the MDM Observatory on Kitt Peak using the OSU CCD spectrograph (CCDS) and covering the spectral range 320.0 to 965.0 nm at a spectral resolution of 0.9 nm.

Woodward, C. E.; Wagner, R. M.; Hounsell, R. A.; Starrfield, S.

2013-10-01

266

Biological and behavioral determinants of fertility in Tierra del Fuego.  

PubMed

The reproductive history of 182 women in postreproductive life or near menopause from the Chilean part of Tierra del Fuego was traced back by means of familial interviews. These postmenopausal women represent the population since almost the beginning of the settlement, and their reproductive years were spent on the island. Path analysis was applied to analyze fertility determinants of these women and to propose a complex model of interconnections among factors. The reproductive history of these women is characterized by a long fertile span, a short childbearing period, and low fertility. Age at menarche is relatively late, and the age of the women at first birth is mainly determined by their late age at marriage. The use of contraception is related to both spacing and stopping behaviors. The late age of women at marriage, the rhythm of conception, and practices of contraception are proposed as the main determinants of fertility in Tierra del Fuego. PMID:15386216

Pascual, J; García-Moro, C E; Hernández, M

2005-05-01

267

El ``Aula del Cel'': astronomy approaching high school  

NASA Astrophysics Data System (ADS)

The “Aula del Cel” is a project carried out by the Astronomical Obsevatory of the University of Valencia, Spain. It offers high-school teachers a tool for teaching Astronomy to 10 to 17 year-old students. Different programs, experiments, and audiovisual presentations have been prepared by a team formed both by professional astronomers and teachers, and are offered in a format chosen to suit each particular age and curriculum group. Special sessions have been designed for students with special needs (for example blind or autistic children) in close contact with the pedagogical teams responsible for their education. Nearly three thousand students have already visited the “Aula del Cel” over the first six months that it has been offered to the school community.

Ortiz-Gil, A.; Gómez Collado, M.; Gallego Calvente, A. T.

268

UTILIZZO DEL TRAMADOLO PER VIA EPIDURALE NEL CANE: CASE REPORT  

Microsoft Academic Search

RIASSUNTO In questo lavoro è stato impiegato e descritto l'utilizzo del Tramadolo per via epidurale durante l'anestesia in un cane che affronta, in successione, tre interventi nella stessa seduta operatoria; il primo in regione perineale seguito da orchiectomia e successivamente l'exeresi di una grossa neoformazione sul gomito. La somministrazione epidurale lombo-sacrale effettuata con Tramadolo a 2mg\\/kg diluito 1:1 in soluzione

Pierre MELANIE

269

Descripción del coronógrafo a ser instalado en Argentina (MICA)  

NASA Astrophysics Data System (ADS)

El ``Coronógrafo de espejo para Argentina'' es un telescopio solar terrestre a ser colocado en el Observatorio Astronómico Félix Aguilar (El Leoncito), antes de finalizar 1996, como parte de un programa de ciencia bilateral entre Alemania y Argentina. Eclipses fotográficos de alta resolución han revelado que la corona solar es altamente estructurada y variable. De hecho, está contínuamente deformada y moldeada por los movimientos convectivos de los extremos de los arcos magnéticos en la fotosfera, estando, en muchas oportunidades, afectada por explosivas liberaciones de energía. MICA, en conjunción con otros telescopios solares espaciales y terrestres, tratará de contribuir al entendimiento de cuestiones fundamentales de la física solar. Entre ellas: cómo la corona está siendo calentada, dónde y cómo el viento solar es acelerado, qué causa los transitorios coronales, etc. Para ello investigará la distribución de los parámetros del plasma y su evolución con el tiempo, la estructura espacial de la corona en fina y gran escala, procesos que ocurren en los transitorios coronales y factores que los disparan, etc. Para responder a estas cuestiones MICA observará la atmósfera solar por sobre el limbo entre 1.1 y 2 radios solares aproximadamente, usando un nuevo tipo de sistema coronográfico que permite suprimir el brillo del disco solar suficientemente bien, tomando las imágenes con una cámara CCD de 1024x1024 pixels, codificada en 12 bits, pudiendo el mismo ser operado en forma remota. En la presente exposición describiremos las características del instrumento, cómo será controlado y qué esperamos observar basados en las imágenes obtenidas por los telescopios de similares características LASCO C1 a bordo del SOHO y PICO (ubicado en el Observatorio de Pic du Midi, Francia).

Stenborg, G.; Francile, C.; Schwenn, R.; Epple, A.; Rovira, M.

270

An erupted migmatite from Cerro del Hoyazo, SE Spain  

Microsoft Academic Search

The almandine-bearing biotite-cordierite-labradorite dacite of the Cerro del Hoyazo is part of the Neogene volcanic range in SE Spain, extending roughly from Cabo de Gata to Cartagena. About 1 vol. % of the lava consists of rock inclusions, measuring over 1 cm, made up of almandine-biotite-sillimanite gneiss, quartz-cordierite gneiss and spinel-cordierite rock. On the ground of their abundance, chemical composition,

H. P. Zeck

1970-01-01

271

Steroidal saponins from the aerial parts of Tribulus alatus Del  

Microsoft Academic Search

Six steroidal glycosides (1–6) were isolated from the aerial parts of Tribulus alatus Del. (Zygophyllaceae), together with one known cholestane, one spirostane, and six flavonol glycosides. Among them, 1 and 2 possess a furostane-type aglycone, 3 and 6 a cholestane structure, and 4 and 5 a spirostane skeleton. Their structural elucidation was accomplished by extensive spectroscopic methods including 1D (1H,

Abeer Temraz; Omayma D. El Gindi; Hazem A. Kadry; Nunziatina De Tommasi; Alessandra Braca

2006-01-01

272

Prenatal Care in the Paso del Norte Border Region  

Microsoft Academic Search

OBJECTIVES: The impact of a Medicaid-managed care system on access to prenatal care was investigated.STUDY DESIGN: Postpartum interviews and medical chart abstractions were conducted among 493 Hispanic women who reside on the El Paso Texas\\/Juarez Mexico border (the Paso del Norte region). Descriptive analysis identified barriers and facilitators to prenatal care. Logistic regression identified the impact of social and demographic

Judith T Fullerton; Carlene Nelson; Rachel Shannon; Julia Bader

2004-01-01

273

L'Aula del Cel: Communicating Astronomy at School Level  

NASA Astrophysics Data System (ADS)

L'Aula del Cel (The "Sky Classroom") is an effort to bring astronomy closer to school children. It is a project led by the Astronomical Observatory of the University of Valencia in collaboration with the regional education authorities. It has been running for two years and it has been a big success, with nearly three thousand students visiting the Aula during the school term.

Ortiz-Gil, A.; Gomez-Collado, M.; Gallego-Calvente, A. T.

2005-12-01

274

Correction: First high resolution ultraviolet (HST/STIS) and supporting optical spectroscopy of V339 Del = Nova Del 2013  

NASA Astrophysics Data System (ADS)

Due to an error in transcription, the distance is given incorrectly in the text of ATel#5409. Along with the typo "Space Telescope Imaging Spectrograph", the text should read: Comparing these STIS UV spectra with high resolution IUE spectra of the CO nova OS And 1986 obtained between 12 days (LWP) and 22 days (SWP) after optical discovery (1986 Dec. 17, 27 respectively) shows a very close agreement such that when independently dereddened, the ratio of V339 Del to OS And between 1200 and 1900A is ~1.5 in flux (similar in the 2000-3000A range but more uncertain because of the earlier date of the long wavelength IUE observation), indicating that the distance to V339 Del is about 4.2 kpc (distance to OS And ~ 5.1 kpc with E(B-V)=0.25+/-0.05, see Schwarz et al.

Shore, S. N.

2013-09-01

275

PLANTAS INTRODUCIDAS EN EL PARQUE NACIONAL TORRES DEL PAINE, CHILE ALIEN PLANTS IN TORRES DEL PAINE NATIONAL PARK, CHILE  

Microsoft Academic Search

We report 61 new records of alien plants for Torres del Paine National Park. The park is located in the Magellan Region (49°21'-51°08'S y 73°07'-74°52'O), with an area of 181.414 ha. The study area has 21 families, 65 genera and 85 alien species. The families with the largest number of species are Poaceae (22 spp.), Asteraceae (11 spp.) and Caryophyllaceae

Erwin Domínguez; Arve Elvebakk; Clodomiro Marticorena; Aníbal Pauchard

276

El Vallès Oriental : problemes induïts per l'artificialització del sòl i la preservació del medi ambient  

Microsoft Academic Search

El paisatge i els recursos naturals al Vallès Oriental estan sotmesos a una forta pressió deguda a la progressiva artificialització del medi. L'article proposa una metodologia per tal d'avaluar l'impacte d'aquesta pressió i les mesures correctores que s'hi apliquen. Així, comença per establir una tipologia d'àrees que permet distingir entre municipis desenvolupats, dinàmics, primaris i desafavorits. A continuació proposa un

Jordi Bertran

2008-01-01

277

Su inversioni del campo elettrico terrestre a cielo sereno e una loro possibile spiegazione  

Microsoft Academic Search

\\u000a Sunto  Viene descritto un andamento anomalo del gradiente di Potenziale al Col d’Olen sul Monte Rosa durante una giornata che, dalle\\u000a condizioni atmosferiche, si poteva giudicare del tutto normale.\\u000a \\u000a Del fenomeno si dà una spiegazione plausibile dimostrando corne sia possibile la formazione di cariche spaziali solo che a\\u000a una certa distanza dalla stazione si trovino nubi o semplici strati di nebbia

G. Aliverti; M. C. Montù

1931-01-01

278

VEGF: A modifier of the del22q11 (DiGeorge) syndrome?  

Microsoft Academic Search

Hemizygous deletion of chromosome 22q11 (del22q11) causes thymic, parathyroid, craniofacial and life-threatening cardiovascular birth defects in 1 in 4,000 infants. The del22q11 syndrome is likely caused by haploinsufficiency of TBX1, but its variable expressivity indicates the involvement of additional modifiers. Here, we report that absence of the Vegf164 isoform caused birth defects in mice, reminiscent of those found in del22q11

Ingeborg Stalmans; Diether Lambrechts; Frederik De smet; Sandra Jansen; Jian Wang; Sunit Maity; Paige Kneer; Maren von der Ohe; Ann Swillen; Christa Maes; Marc Gewillig; Daniel G. M. Molin; Peter Hellings; Thurid Boetel; Maartin Haardt; Veerle Compernolle; Mieke Dewerchin; Stephane Plaisance; Robert Vlietinck; Beverly Emanuel; Adriana C. Gittenberger-de Groot; Peter Scambler; Bernice Morrow; Deborah A. Driscol; Lieve Moons; Camila V. Esguerra; Geert Carmeliet; Annett Behn-Krappa; Koen Devriendt; Désiré Collen; Simon J. Conway; Peter Carmeliet

2003-01-01

279

The highly prevalent BRCA2 mutation c.2808_2811del (3036delACAA) is located in a mutational hotspot and has multiple origins.  

PubMed

BRCA2-c.2808_2811del (3036delACAA) is one of the most reported germ line mutations in non-Ashkenazi breast cancer patients. We investigated its genetic origin in 51 Spanish carrier families that were genotyped with 11 13q polymorphic markers. Three independent associated haplotypes were clearly distinguished accounting for 23 [west Castilla y León (WCL)], 20 [east Castilla y León (ECL)] and 6 (South of Spain) families. Mutation age was estimated with the Disequilibrium Mapping using Likelihood Estimation software in a range of 45-68 and 45-71 generations for WCL and ECL haplotypes, respectively. The most prevalent variants, c.2808_2811del and c.2803G > A, were located in a double-hairpin loop structure (c.2794-c.2825) predicted by Quikfold that was proposed as a mutational hotspot. To check this hypothesis, random mutagenesis was performed over a 923 bp fragment of BRCA2, and 86 DNA variants were characterized. Interestingly, three mutations reported in the mutation databases (c.2680G > A, c.2944del and c.2957dup) were replicated and 20 affected the same position with different nucleotide changes. Moreover, five variants were placed in the same hairpin loop of c.2808_2811del, and one affected the same position (c.2808A > G). In conclusion, our results support that at least three different mutational events occurred to generate c.2808_2811del. Other highly prevalent DNA variants, such as BRCA1-c.68_69delAG, BRCA2-c.5946delT and c.8537delAG, are concentrated in hairpin loops, suggesting that these structures may represent mutational hotspots. PMID:23929434

Infante, Mar; Durán, Mercedes; Acedo, Alberto; Sánchez-Tapia, Eva María; Díez-Gómez, Beatriz; Barroso, Alicia; García-González, María; Feliubadaló, Lídia; Lasa, Adriana; de la Hoya, Miguel; Esteban-Cardeñosa, Eva; Díez, Orland; Martínez-Bouzas, Cristina; Godino, Javier; Teulé, Alexandre; Osorio, Ana; Lastra, Enrique; González-Sarmiento, Rogelio; Miner, Cristina; Velasco, Eladio A

2013-08-08

280

CHEK2 1100delC and Del5395bp mutations in BRCA-negative individuals from Serbian hereditary breast and ovarian cancer families.  

PubMed

Purpose: Hereditary breast/ovarian cancer (HBOC) occurs in families with several members affected. Most HBOC is caused by mutations in high penetrance BRCA genes accounting for about 5% of all breast cancers. There is a large number of genes with moderate or low penetrance, contributing to non-BRCA aggregation of breast and ovarian cancers. In the present study, we evaluated the presence and frequency of 1100delC and Del5395 mutations in CHEK2 gene in Serbian BRCA-negative HBOC cases. Methods: We analyzed 57 BRCA-negative subjects from high risk breast/ovarian cancer families from Serbia for CHEK2 1100delC and Del5395 mutations. We used two multiplex allele specific PCR in order to detect them. All suspected positive cases were compared with controls and confirmed by DNA sequencing. Results: 1100delC was not detected in the tested group. However, we detected one Del5395 mutation in a female diagnosed with breast cancer at the age of 32 and with apparent family history of breast cancer (her mother and sister were diagnosed with breast cancer at 42 and 39 years of age, respectively). The frequency of Del5395 mutation in our tested group was 1.7% (1/57). Conclusions: 1100delC variant in CHEK2 gene was not present in the tested subjects from HBOC families in Serbia. However, the finding of Del5395 mutation does not allow us to discard a possible involvement of this gene in breast cancer susceptibility in Serbian population. It would be of great interest to assess the distribution of this large deletion in other countries from the Balkan region in order to assess its geographical distribution and possible founder effect. PMID:24065469

Krivokuca, A; Dobricic, J; Brankovic-Magic, M

281

Distribución del continuo ultravioleta e infrarrojo en estrellas Be  

NASA Astrophysics Data System (ADS)

Estamos interesados en interpretar los excesos de flujo infrarrojo y/o ultravioleta observados en estrellas Be con respecto al de estrellas B normales, así como su posible correlación. Una envoltura rodeando la estrella distingue una estrella Be de una B normal; para explicar las observaciones es necesario entonces tener en cuenta todos los posibles procesos físicos que en ella tienen lugar. Adoptando un modelo atmosférico específico, hemos estudiado el proceso de recombinación dielectrónica de metales como MgII y FeII, teniendo en cuenta la posible influencia de la opacidad del medio en el flujo emitido.

Cruzado, A.; Ringuelet, A.; di Rocco, H.

282

An erupted migmatite from Cerro del Hoyazo, SE Spain  

Microsoft Academic Search

The almandine-bearing biotite-cordierite-labradorite dacite of the Cerro del Hoyazo is part of the Neogene volcanic range\\u000a in SE Spain, extending roughly from Cabo de Gata to Cartagena.\\u000a \\u000a About 1 vol. % of the lava consists of rock inclusions, measuring over 1 cm, made up of almandine-biotite-sillimanite gneiss,\\u000a quartz-cordierite gneiss and spinel-cordierite rock. On the ground of their abundance, chemical composition,

H. P. Zeck

1970-01-01

283

X-ray detection of Nova Del 2013 with Swift  

NASA Astrophysics Data System (ADS)

Continuous X-ray monitoring by Swift of Nova Del 2013 (see CBET #3628) shows an increase of X-ray emission at the source location compared to previous observations (ATEL #5283, ATEL #5305) during a 3.9 ksec observation at UT 2013-08-22 12:05. With the XRT instrument operating in window timing mode, 744 counts were extracted from a 50 pixel long source region and 324 counts from a similar box for a background region, resulting in a 13-sigma detection with a net count rate of 0.11±0.008 counts/sec.

Castro-Tirado, Alberto J.; Martin-Carrillo, Antonio; Hanlon, Lorraine

2013-08-01

284

Optical photometry and spectroscopy of Nova Del 2013  

NASA Astrophysics Data System (ADS)

We report optical BV photometry and spectroscopy of Nova Del 2013, carried out between August 14.88 UT and August 15.08 UT. Using a 60 cm Cassegrain telescope at the Nicolaus Copernicus University Observatory (Torun, Poland) we estimated the V brightness of the Nova to be 6.31+/-0.02 mag and 6.18+/-0.03 mag on Aug. 14.94 UT and Aug. 15.02 UT respectively. HD 194113 (F2, V=8.00 mag) was used as a comparison star.

Tomov, T.; Ilkiewicz, K.; Swierczynski, E.; Belcheva, M.; Dimitrov, D.

2013-08-01

285

Crueldad, simulacion, absurdo y amenaza: Barbarie del drama moderno y tradicion hermetica en los esperpentos de Ramon del Valle-Inclan  

Microsoft Academic Search

The purpose of this dissertation is to demonstrate that the esperpentos by the Spanish playwright Ramón del Valle-Inclán (1866–1936), represent a culminating moment of theatrical precepts of modern European drama, while perpetuating the ancient esoteric traditions of the Iberian Peninsula. Focusing on four plays—Los cuernos de Don Friolera (1920), Luces de Bohemia (1921), Las galas del difunto (1926) y La

Barbara Safille

2004-01-01

286

DISEÑO DE UNA PLANTA PILOTO PARA EL TRATAMIENTO DEL RESIDUAL DE UNA SALINA  

Microsoft Academic Search

En el proceso de extracción de cloruro de sodio a partir de agua de mar queda una salmuera residual rica en sales de sodio y magnesio que al ser tratadas químicamente y al ser evaporada permite recuperar aproximadamente el 75% del cloruro de sodio que queda en dicho residual, lo que puede reducir la contaminación ambiental del litoral y generar

Mariana Estela Colombat Rodríguez; Amada Lissett Colombat Rodríguez

2011-01-01

287

ALERTINF: MODELLO DI PREVISIONE DELLE EMERGENZE PER IL CONTROLLO DELLE INFESTANTI DEL MAIS IN VENETO  

Microsoft Academic Search

La gestione delle infestanti è una pratica fondamentale nella coltivazione del mais. Il tempo di comparsa delle malerbe influenza la competizione tra infestante e specie coltivata determinando l'entità del danno che le malerbe possono provocare in termini di resa della coltura. La capacità di prevedere le emergenze delle malerbe può aiutare ad ottimizzare i tempi di controllo, può aumentare l'efficacia

Roberta Masin; Giuliano Cacciatori; Maria Clara Zuin; Giuseppe Zanin

288

Estrategia para potenciar el desarrollo del talento en los escolares de la educación primaria  

Microsoft Academic Search

El desarrollo del talento en el mundo es una polémica que cobra auge en la actualidad. Cuba, país en vía de desarrollo brinda a este tema especial atención. Por su importancia y novedad, la presente investigación se centra en cómo potenciar el desarrollo del talento en la Educación Primaria. Con un enfoque histórico cultural la estrategia propuesta como vía de

Niurkis Lozano Cabrera

2011-01-01

289

ALCANCES Y LIMITACIONES DE LA TEORÍA DEL APEGO Y LA BASE SEGURA  

Microsoft Academic Search

El origen de la teoría del apego se remonta a los trabajos etológicos de Lorenz con patos silvestres. Según la observación de este autor, se notaba en los patos cierto apego entre la madre y sus crías minutos después de los nacimientos; dicho lazo duraría gran parte de la vida biológica del animal (Bowlby, 1986; 1989). Con estas observaciones en

Maximiliano Korstanje

2009-01-01

290

Contrastación del modelo de in versión de Rusbult en una muestra de casados y divorciados  

Microsoft Academic Search

Este estudio examina la capacidad del modelo de in versión propuesto por Rusbult para diferenciar en - tre individuos en relaciones estables e individuos en relaciones inestables, así como la capacidad de las variables del modelo (satisfacción matrimonial, in versión y relaciones alternati vas) para predecir el compromiso y la estabilidad de las relaciones matrimoniales. Los datos sobre las varia

David Martínez-Íñigo

2000-01-01

291

El Instituto Nacional del Cáncer publica nuevo atlas de mortalidad por cáncer  

Cancer.gov

El Instituto Nacional del Cáncer (NCI, por sus siglas en inglés) ha publicado un nuevo atlas, el Atlas de Mortalidad por Cáncer en los Estados Unidos, 1950-94, que muestra los patrones geográficos de las tasas de mortalidad por cáncer durante más de cuatro décadas, en más de 3.000 condados a lo largo del país.

292

61. M.C., photographer June 22, 1951 KLAMATH, DEL NORTE COUNTY, ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

61. M.C., photographer June 22, 1951 KLAMATH, DEL NORTE COUNTY, SECTION A, HIGHWAY 1. DN-1-A #452 NORTHERLY THROUGH KLAMATH FROM STA. 325+50, 6/22/51, M.C. Stamped office copy. - Redwood National & State Parks Roads, California coast from Crescent City to Trinidad, Crescent City, Del Norte County, CA

293

Indicador espacial del metabolismo urbano. Huella Ecológica de la ciudad de Tandil, Argentina  

Microsoft Academic Search

Además de problemas urbanos evidentes -contaminación, residuos, etc.-, existe un problema asociado a la propia dinámica del sistema urbano que es de interés: la apropiación creciente de ecosistemas productivos externos destinados a satisfacer los requerimientos del metabolismo urbano de la ciudad de Tandil, Argentina. Se emplea para su cálculo, la Huella Ecológica (HE) (Rees 1996a; Wackernagel 1996) que permite evaluar

Elsa Marcela Guerrero; Fernando Guiñirgo

2008-01-01

294

Experience the magic of light and color: outreach activity by Universidad del Valle student chapter  

Microsoft Academic Search

During 2007, the Universidad del Valle Student Chapter presented a proposal for developing an educational outreach activity for children from an underprivileged zone to the Optical Society of America Foundation (OSAF) and to SPIE. The activity was carried out jointly by OSA and SPIE Universidad del Valle Student Chapters in the hillsides of Santiago de Cali, in a zone known

Claudia Valdes; Camilo Reyes; Alberto Osorio; Efrain Solarte

2010-01-01

295

Quattordici Punti di accordo con i rapporti ufficiali governativi sulla distruzione del World Trade Center  

Microsoft Academic Search

I rapporti di FEMA e NIST contengono la versione ufficiale sulla distruzione del World Trade Center, avvenuta l'11 settembre 2001. In questa lettera vogliamo porre le basi per una discussione costruttiva e chiarificatrice, focalizzandoci sui punti di comune accordo con FEMA e NIST, e refutando nel contempo diverse credenze errate sui crolli del WTC. L'undici settembre 2001 le Torri Gemelle

Steven E. Jones; Frank M. Legge; Kevin R. Ryan; Anthony F. Szamboti; James R. Gourley

296

Estudio del Selenio y la Vitamina E para Prevenir el Cáncer (SELECT): preguntas y respuestas  

Cancer.gov

SELECT sigue evaluando los efectos de los suplementos aunque los participantes ya no toman los suplementos del estudio. El Comité de Vigilancia de Datos y Seguridad del estudio, que opera con plena independencia, descubrió que el selenio y la vitamina E, tomados solos o juntos por un promedio de 5 años, no impide el cáncer de próstata.

297

Estudio del Selenio y la Vitamina E para Prevenir el Cáncer (SELECT)  

Cancer.gov

SELECT sigue evaluando los efectos de los suplementos aunque los participantes ya no toman los suplementos del estudio. El Comité de Vigilancia de Datos y Seguridad del estudio, que opera con plena independencia, descubrió que el selenio y la vitamina E, tomados solos o juntos por un promedio de 5 años y medio, no impiden el cáncer de próstata.

298

33 CFR 334.110 - Delaware Bay off Cape Henlopen, Del.; naval restricted area.  

Code of Federal Regulations, 2013 CFR

...2013-07-01 2013-07-01 false Delaware Bay off Cape Henlopen, Del.; naval...RESTRICTED AREA REGULATIONS § 334.110 Delaware Bay off Cape Henlopen, Del.; naval...Beginning at a point on the south shore of Delaware Bay at longitude 75°06â²12â³; thence to latitude...

2013-07-01

299

33 CFR 162.200 - Marina del Rey, Calif.; restricted area.  

Code of Federal Regulations, 2010 CFR

...Waters 2 2010-07-01 2010-07-01 false Marina del Rey, Calif.; restricted area. 162.200 Section 162.200...INLAND WATERWAYS NAVIGATION REGULATIONS § 162.200 Marina del Rey, Calif.; restricted area. (a) The area ....

2010-07-01

300

33 CFR 162.200 - Marina del Rey, Calif.; restricted area.  

Code of Federal Regulations, 2010 CFR

...Waters 2 2009-07-01 2009-07-01 false Marina del Rey, Calif.; restricted area. 162.200 Section 162.200...INLAND WATERWAYS NAVIGATION REGULATIONS § 162.200 Marina del Rey, Calif.; restricted area. (a) The area ....

2009-07-01

301

C.35delG/ GJB2 and del(GJB6-D13S1830) mutations in Croatians with prelingual non-syndromic hearing impairment  

PubMed Central

Background C.35delG/GJB2 mutation is the most frequent genetic cause of deafness in Caucasians. Another frequent mutation in some Caucasian populations is del(GJB6-D13S1830). Both GJB2 and GJB6 genes belong to the same DFNB1 locus and when the two mutations are found in combination in a hearing-impaired person, a digenic pattern of inheritance is suggested. Methods We examined 63 Croatian subjects (25 familial and 38 sporadic cases) with prelingual non-syndromic hearing impairment by polymerase chain reaction for the presence of the c.35delG/GJB2 and the del(GJB6-D13S1830) mutations. Results Of the 63 unrelated hearing-impaired subjects, the mutation c.35delG/GJB2 was found in 21 subjects (33.3%). In 5 of them the mutation was found in the heterozygous state, all of them being compound heterozygotes, as sequencing revealed a second mutation within the coding region of the gene in 3 subjects, and a splice site mutation in 2 subjects. The del(GJB6-D13S1830) mutation was not found in the investigated hearing-impaired Croatian subjects. Conclusion Our results contribute to the knowledge of geographic distribution and population genetics of the GJB2 and GJB6 mutations in the Europeans.

Medica, Igor; Rudolf, Gorazd; Balaban, Manuela; Peterlin, Borut

2005-01-01

302

33 CFR 162.40 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal).  

Code of Federal Regulations, 2013 CFR

...2013-07-01 false Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal). 162.40 Section 162...REGULATIONS § 162.40 Inland waterway from Delaware River to Chesapeake Bay, Del. and...

2013-07-01

303

33 CFR 207.100 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware...  

Code of Federal Regulations, 2013 CFR

...2013-07-01 false Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and... § 207.100 Inland waterway from Delaware River to Chesapeake Bay, Del. and...

2013-07-01

304

33 CFR 207.100 - Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware...  

Code of Federal Regulations, 2010 CFR

...2010-07-01 false Inland waterway from Delaware River to Chesapeake Bay, Del. and Md. (Chesapeake and Delaware Canal); use, administration, and... § 207.100 Inland waterway from Delaware River to Chesapeake Bay, Del. and...

2010-07-01

305

Disruption of cytokeratin-8 interaction with F508del-CFTR corrects its functional defect  

PubMed Central

We have previously reported an increased expression of cytokeratins 8/18 (K8/K18) in cells expressing the F508del mutation of cystic fibrosis transmembrane conductance regulator (CFTR). This is associated with increased colocalization of CFTR and K18 in the vicinity of the endoplasmic reticulum, although this is reversed by treating cells with curcumin, resulting in the rescue of F508del-CFTR. In the present work, we hypothesized that (i) the K8/K18 network may interact physically with CFTR, and that (ii) this interaction may modify CFTR function. CFTR was immunoprecipitated from HeLa cells transfected with either wild-type (WT) CFTR or F508del-CFTR. Precipitates were subjected to 2D-gel electrophoresis and differential spots identified by mass spectrometry. K8 and K18 were found significantly increased in F508del-CFTR precipitates. Using surface plasmon resonance, we demonstrate that K8, but not K18, binds directly and preferentially to the F508del over the WT human NBD1 (nucleotide-binding domain-1). In vivo K8 interaction with F508del-CFTR was confirmed by proximity ligation assay in HeLa cells and in primary cultures of human respiratory epithelial cells. Ablation of K8 expression by siRNA in F508del-expressing HeLa cells led to the recovery of CFTR-dependent iodide efflux. Moreover, F508del-expressing mice topically treated with K8-siRNA showed restored nasal potential difference, equivalent to that of WT mice. These results show that disruption of F508del-CFTR and K8 interaction leads to the correction of the F508del-CFTR processing defect, suggesting a novel potential therapeutic target in CF.

Colas, Julien; Faure, Grazyna; Saussereau, Emilie; Trudel, Stephanie; Rabeh, Wael M.; Bitam, Sara; Guerrera, Ida Chiara; Fritsch, Janine; Sermet-Gaudelus, Isabelle; Davezac, Noelie; Brouillard, Franck; Lukacs, Gergely L.; Herrmann, Harald; Ollero, Mario; Edelman, Aleksander

2012-01-01

306

High precision ages from the Torres del Paine Intrusion, Chile  

NASA Astrophysics Data System (ADS)

The upper crustal bimodal Torres del Paine Intrusion, southern Chile, consists of the lower Paine-Mafic- Complex and the upper Paine-Granite. Geochronologically this bimodal complex is not well studied except for a few existing data from Halpern (1973) and Sanchez (2006). The aim of this study is to supplement the existing data and to constrain the age relations between the major magmatic pulses by applying high precision U-Pb dating on accessory zircons and 40Ar/39Ar-laser-step-heating-ages on biotites from the Torres del Paine Intrusion. The magmatic rocks from mafic complex are fine to medium-grained and vary in composition from quartz- monzonites to granodiorites and gabbros. Coarse-grained olivine gabbros have intruded these rocks in the west. The granitic body is represented by a peraluminous, biotite-orthoclase-granite and a more evolved leucocratic granite in the outer parts towards the host-rock. Field observations suggest a feeder-zone for the granite in the west and that the granite postdates the mafic complex. Two granite samples of the outermost margins in the Northeast and South were analyzed. The zircons were dated by precise isotope-dilution U-Pb techniques of chemically abraded single grains. The data are concordant within the analytical error and define weighted mean 206/238U ages of 12.59 ± 0.03 Ma and 12.58 ± 0.01 Ma for the two samples respectively. A 40Ar/39Ar-age for the second sample yield a date of 12.37 ± 0.11 Ma. Three 40Ar/39Ar -ages of biotites were obtained for rocks belonging to the mafic complex. A hbl-bio- granodiorite from the central part, approximately 150 m below the subhorizontal contact with the granite, gives an age of 12.81 ± 0.11 Ma. A hbl-bio-granodiorite and an olivine-gabbro west of the feeder-zone date at 12.42 ± 0.14 Ma and 12.49 ± 0.11 Ma, respectively. The obtained older age of 12.81 Ma for the granodiorite in the central part is consistent with structural relationships of brittle fracturing of the mafic complex by the granite and we conclude that some parts of the mafic complex were emplaced before the granite. The well defined 206/238U-age for zircons and the slightly younger 40Ar/39Ar -ages for biotites of both rock suites show that emplacement and cooling of the Torres del Paine Intrusion took place in a relatively short time-frame. Halpern, 1973, Geological Society of America Bulletin, 84/7: 2407-2422. Sanchez et.al., 2006. V SSAGI, Punta del Este, April 2006.

Michel, J.; Baumgartner, L.; Cosca, M.; Ovtcharova, M.; Putlitz, B.; Schaltegger, U.

2006-12-01

307

Numerical Kähler-Einstein Metric on the Third del Pezzo  

NASA Astrophysics Data System (ADS)

The third del Pezzo surface admits a unique Kähler-Einstein metric, which is not known in closed form. The manifold’s toric structure reduces the Einstein equation to a single Monge-Ampère equation in two real dimensions. We numerically solve this nonlinear PDE using three different algorithms, and describe the resulting metric. The first two algorithms involve simulation of Ricci flow, in complex and symplectic coordinates respectively. The third algorithm involves turning the PDE into an optimization problem on a certain space of metrics, which are symplectic analogues of the “algebraic” metrics used in numerical work on Calabi-Yau manifolds. Our algorithms should be applicable to general toric manifolds. Using our metric, we compute various geometric quantities of interest, including Laplacian eigenvalues and a harmonic (1,1)-form. The metric and (1,1)-form can be used to construct a Klebanov-Tseytlin-like supergravity solution.

Doran, Charles; Headrick, Matthew; Herzog, Christopher P.; Kantor, Joshua; Wiseman, Toby

2008-09-01

308

Renewable energy load assessment for Boquillas Del Carmen Coahuila, Mexico  

SciTech Connect

This report outlines the estimates that were made in 1992 of the potential load requirements for Boquillas del Carmen, a small Mexican village on the northern border of the state of Coahuila, Mexico near Big Bend National Park in southern Texas. The study was made to help determine the possibility that village might be electrified by solar or wind energy. Various estimates of are given of the potential load based on estimates ranging from basic use of lights, radio, television, and small household appliances to microwave ovens, refrigerators, and direct evaporative coolers. The low-energy consumption case was estimated to be at 23.0 kWh/month per residence per month, and the high-energy consumption case (with cooling) was 140.7 kWh/month per residence. On average, the typical residence is occupied by five individuals.

Foster, R. [Southwest Technology Development Institute, Las Cruces, NM (United States)

1995-08-01

309

Near-IR Photometry of Nova Del 2013  

NASA Astrophysics Data System (ADS)

Subjects: Infra-Red, Nova We report Near-IR photometry of Nova Del 2013 taken with the 0.76-m infrared telescope at the University of Minnesota's O'Brien Observatory (Marine on St. Croix, Minnesota, USA). RIJHKLM photometry were obtained on August 21.17 and 23.13 UT using an AsSi bolometer. Vega (alpha Lyrae) was used as the standard star. Our photometry show: August 21.17 UT: R = 5.1 +/- 0.1, I = 4.6 +/- 0.1, J = 4.6 +/- 0.1, H = 4.1 +/- 0.1, K = 3.9 +/- 0.1, L = 2.7 +/- 0.2, M = +2.2 +/- 0.2.

Cass, C. A.; Carlon, L. R.; Corgan, T. D.; Dykhoff, A. D.; Gehrz, D. R.; Shenoy, P. D.

2013-08-01

310

Impacto de las Tecnologías de la Información y las Comunicaciones (TIC) en el desarrollo y la competitividad del país  

Microsoft Academic Search

La primera parte de este estudio caracteriza al sector de telecomunicaciones en Colombia, comparando a Colombia con otros países y regiones del mundo, mostrando la evolución de algunos de los principales indicadores de telecomunicaciones. En la segunda parte se hacen estimaciones cuantitativas del impacto socioeconómico del sector en la economía colombiana, a través de dos herramientas: la metodología insumo-producto y

Juan Benavides; Felipe Castro; Lina Devis; Mauricio Olivera

2011-01-01

311

EL PAPEL DE LOS AUXILIARES DE LA JUSTICIA EN VÍAS DE LA IMPLEMENTACIÓN DEL SISTEMA ACUSATORIO EN PANAMÁ  

Microsoft Academic Search

Este artículo trata sobre el papel preponderante que juegan los Auxiliares de la Justicia que cobra mayor relevancia dentro del engranaje del modelo del Sistema Acusatorio, en los Procesos Orales, garantista en Contra posición con el actual Sistema Inquisitivo destacando el trabajo, capacidad y preponderancia de Estos colaboradores muchas veces anónimos en el Sistema actual, pero que serán de suma

Ivanovha Lamela

2011-01-01

312

Deglacial palaeoclimate at Puerto del Hambre, subantarctic Patagonia, Chile  

NASA Astrophysics Data System (ADS)

The primary objective of this study is to further substantiate multistep climatic forcing of late-glacial vegetation in southern South America. A secondary objective is to establish the age of deglaciation in Estrecho de Magallanes-Bahía Inútil. Pollen assemblages at 2-cm intervals in a core of the mire at Puerto del Hambre (53°3621S, 70°5553W) provide the basis for reconstructing the vegetation and a detailed account of palaeoclimate in subantarctic Patagonia. Chronology over the 262-cm length of core is regulated by 20 AMS radiocarbon dates between 14 455 and 10 089 14C yr BP. Of 13 pollen assemblage zones, the earliest representing the Oldest Dryas chronozone (14 455-13 000 14C yr BP) records impoverished steppe with decreasing frequencies and loss of southern beech (Nothofagus). Successive 100-yr-long episodes of grass/herbs and of heath (Empetrum/Ericaceae) before 14 000 14C yr BP infer deglacial successional communities under a climate of increased continentality prior to the establishment of grass-dominated steppe. The Bølling-Allerød (13 000-11 000 14C yr BP) is characterised by mesic grassland under moderating climate that with abrupt change to heath dominance after 12 000 14C yr BP was warmer and not as humid. At the time of the Younger Dryas (11 000-10 000 14C yr BP), grass steppe expanded with a return of colder, more humid climate. Later, with gradual warming, communities were invaded by southern beech. The Puerto del Hambre record parallels multistep, deglacial palaeoclimatic sequences reported elsewhere in the Southern Andes and at Taylor Dome in Antarctica. Deglaciation of Estrecho de Magallanes-Bahía Inútil is dated close to 14 455 14C yr BP, invalidating earlier dates of between 15 800 and 16 590 14C yr BP.

Heusser, Calvin J.; Heusser, Linda E.; Lowell, Thomas V.; Moreira M., Andrés; Moreira M., Simón

2000-02-01

313

[Epidemiology of dog bite lesions in Tierra del Fuego, Argentina].  

PubMed

The coexistence between man and dog has resulted in mutual benefits during thousands of years, nevertheless some problems have recently arisen where bite injuries have an important role. The aim of this work was to describe the epidemiological characteristics of dog bite lesions which occurred during a year in Tierra del Fuego. A descriptive observational, transversal study of patients who were admitted with dog bite injuries to clinic and pediatric emergency services of hospitals and health centers was carried out between 3/1/05 and 3/1/06. A total of 382 records were made. The children group represents 49.5%. The group between 5 and 9 year-old was the most affected (44.4%; IC95% 37.2-51.8). In the adult group, that between 15 and 24 year-old was the most affected (29.5%; IC95% 23.2-36.5). More lesions were registered in men (57.6%) than in women (42.4%) (chi2 = 8.6, p = 0.003). During spring and summer months, 56.5% of the lesions were registered. A 72.8% of the incidents occurred on the public highway. Dogs of big size were responsible of 49.7% (IC95% 44.6-54.9) of the injuries. The 89.8% of the incidents were caused by another person's dog. Of the lesions, 55.8% (IC95% 50.6-60.8) were registered in lower extremities and 11% (IC95% 8.1-14.7) in head and neck. These data show that dog bite lesions affect the health and impact in the quality of life of the population of Tierra del Fuego, Argentina. PMID:18416313

Zanini, Fabián; Padinger, Patricia; Elissondo, María C; Pérez, Héctor

2008-01-01

314

FISH Analysis for del6q21 and del17p13 in B-cell Chronic Lymphocytic Leukemia in Iranians  

PubMed Central

Background B-cell chronic lymphocytic leukemia (B-CLL) is the most common leukemia in the Western world. Major progress has been made in assessing typical chromosomal abnormalities and recognition of the correlation of these chromosomal abnormalities with laboratory features and clinical course of the disease. The most frequent genomic changes are deletions at 13q14, 11q22-23 and 17p13 and trisomy of chromosome 12. Objectives The aim of this study was to investigate the frequency of chromosomal aberrations in B-CLL patients’ peripheral blood and/or bone marrow using a molecular cytogenetic method, interphase fluorescence in situ hybridization (I-FISH) and to evaluate the correlation between these genomic changes and clinical findings. Patients and Methods I-FISH analyses were performed on bone marrow and blood samples of 66 B-CLL patients. Results Deletion of 17p13 was found in 11 (16.6%) and deletion 6q21 was present in 5 (7.5%). Statistical analyses were performed to investigate the correlation of these molecular-cytogenetic findings with family history, Rai staging and CD38 marker. No clear differences in distribution was noted for del17p13 and del6q21 among patients with and without family history, and no direct correlation was noted between these genomic changes and CD38 marker, but the correlation of del17p13 and Rai stage was significant. There was a high frequency of Rai stage II within del17p13 patients. Conclusions It was demonstrated that the presence of del6q21 in B-CLL patients indicates poor prognosis and on the contrary, presence of del17p13 points at the good prognostic value of the disease.

Teimori, Hossein; Ashoori, Saeede; Akbari, Mohamad Taghi; Mojtabavi Naeini, Marjan; Hashemzade Chaleshtori, Morteza

2013-01-01

315

33 CFR 162.35 - Channel of Christina River, Del.; navigation.  

Code of Federal Regulations, 2013 CFR

...Christina River, Del.; navigation. (a) That vessels of over 20 tons capacity, propelled by machinery, shall not proceed at any time within the limits of these waters at a greater speed than 8 statute miles per...

2013-07-01

316

Intestinal Parasites of Man in Agusan del Norte, Philippines with Emphasis on Schistosomiasis and Capillariasis.  

National Technical Information Service (NTIS)

A total of 1,920 fecal specimens from residents of seven villages in Agusan del Norte Province, Mindanao, Philippines were examined for schistosomes and common intestinal parasites. Schistosoma japonicum eggs were found in 14% of the samples. Infections i...

W. P. Carney R. Nocun A. G. Vergel I. Pagaran A. Mercado

1987-01-01

317

Notes on the Geochemical Survey in Zamboanga Del Norte - Misamis Occidental, Mindanao, Philippines.  

National Technical Information Service (NTIS)

Stream sediment and panned heavy mineral surveys were conducted in Zamboanga del Norte and Misamis Occidental provinces in northwestern Mindanao. A total of 70 stream sediments and 64 panned heavy mineral concentrates were obtained along active streams dr...

G. Santos J. E. Seguis L. Carino P. Escalada L. Antonio

1983-01-01

318

Del Bueno Amends Recall of Queso Fresco Cheese Because of Possible Health Risk  

NASA Website

Del Bueno of Grandview, WA is recalling all size packages of Queso Fresco Fresh Cheese because it has the potential to be contaminated with Listeria monocytogenes, an organism which can cause serious and sometimes fatal infections in young children, ...

319

Elementos para una reforma del sector público en el contexto de una economía de mercado  

Microsoft Academic Search

En este trabajo se propone un conjunto de elementos básicos que deben ser considerados para el diseño de cualquier estrategia de reforma del sector público en el Perú, abordando cuestiones de carácter económico, social, político y de organización institucional.

Claudio Herzka

1996-01-01

320

Geostatistical investigations of rock masses. The Sierra del Medio Case (Argentina).  

National Technical Information Service (NTIS)

The geostatistical tehniques applied for the selection of a minimun fracturation volume in Sierra del Medio allow to quantify and qualify the variability of mechanic characteristics and density of fracture and also the level of reliability in estimations....

J. A. Matar M. A. Sarquis J. P. Girardi G. H. Tabbia

1987-01-01

321

The Ecology and Distribution of Rock-Boring Pelecypods off Del Monte Beach, Monterey, California.  

National Technical Information Service (NTIS)

Divers using SCUBA gear gathered and identified rock-boring pelecypods found in the subtidal outcrops of Monterey silicious shale off Del Monte Beach, Monterey, California. Underwater photographs were taken of all the recognizable species present. A speci...

G. S. Booth

1972-01-01

322

Estudio multiespectral del remanente de supernova W 28  

NASA Astrophysics Data System (ADS)

Se presentan observaciones en continuo de radio con muy alta resolución angular y sensibilidad del remanente de supernova (RSN) W28. Las observaciones fueron realizadas con el interferómetro Very Large Array (Estados Unidos), en las configuraciones C y D, en 1415 y 324 MHz. Por la gran extensión de este remanente (diámetro ~ 1o), las observaciones en 1415 MHz se realizaron en modo mosaico, combinando 48 apuntamientos diferentes en una única imagen. En ambas frecuencias los datos se procesaron con el agregado de observaciones de menor resolución angular, a fin de recuperar información en todas las frecuencias espaciales y obtener estimaciones precisas de densidad de flujo. Las imágenes resultantes muestran por primera vez la presencia de extensiones con forma de arco, cuya naturaleza se investiga. Las observaciones en radio se comparan con una imagen óptica de W28, y con la emisión en rayos X, observada con el satélite ROSAT. Este remanente muestra una clara morfología híbrida, tipo cáscara en frecuencias de radio y de centro lleno en las bandas óptica y de rayos X blandos.

Dubner, G.; Velázquez, P.; Castelletti, G.

323

Paso del Norte ozone study VOC measurements, 1996  

SciTech Connect

The results of VOC determinations of ambient air samples collected at surface air quality monitoring sites and near sources of interest on the US and Mexican side of the border during six weeks of the 1996 Paso del Norte Ozone Study are reported. Carbonyl samples were collected on DNPH impregnated cartridges at three surface sites and analyzed by HPLC to quantify 13, C-1 to C-8 species. Whole air samples were collected in electro-polished stainless steel canisters which were returned to laboratory for determination of C-2 to C-10+ hydrocarbons by cryogenic preconcentration capillary gas chromatography with flame ionization detection (gc-fid). Several sources were sampled: rush hour traffic, propane-powered bus exhaust, automobile paint shop emissions, propane fuel, petroleum refinery, and industrial manufacturing site. Spatial and temporal characteristics of VOC species concentrations and compositions are presented. Overall surface TNMOC values ranged from 0.1 to 3.4 ppmC with the highest concentrations recorded in the morning at three vehicle-dominated sites, two in Cuidad Juarez and one in downtown El Paso. Toluene in El Paso samples and propane, which is used as a cooking and transportation fuel in Cuidad Juarez, were the most abundant hydrocarbons.

Seila, R.L.; Main, H.; Arriaga, J.L.; Martinez, G.V.; Ramadan, A.B.

1999-01-01

324

Paso del Norte ozone study VOC measurements, 1996  

SciTech Connect

The results of VOC determinations of ambient air samples collected at surface air quality monitoring sites and near sources of interest on the US and Mexican side of the border during six weeks of the 1996 Paso del Norte Ozone Study are reported. Carbonyl samples were collected on DNPH impregnated cartridges at three surface sites and analyzed by HPLC to quantify 13, C-1 to C-8 species. Whole air samples were collected in electro-polished stainless steel canisters which were returned to laboratory for determination of C-2 to C-10+ hydrocarbons by cryogenic preconcentration capillary gas chromatography with flame ionization detection (gc-fid). Several sources were sampled: rush hour traffic, propane-powered bus exhaust, automobile paint shop emissions, propane fuel, petroleum refinery, and industrial manufacturing site. Spatial and temporal characteristics of VOC species concentrations and compositions are presented. Overall surface TNMOC values ranged from 0.1 to 3.4 ppmC with the highest concentrations recorded in the morning at three vehicle-dominated sites, two in Cuidad Juarez and one in downtown El Paso. Toluene in El Paso samples and propane, which is used as a cooking and transportation fuel in Cuidad Juarez, were the most abundant hydrocarbons.

Seila, R.L.; Main, H.; Arriaga, J.L.; Martinez, G.V.; Ramadan, A.B.

1999-11-01

325

``Campo del Cielo'' Meteorites: Astronomical Heritage and Cultural Colonialism  

NASA Astrophysics Data System (ADS)

In the province of Chaco, Argentina, there is a very unique dispersion of metallic meteorites called ``Campo del Cielo''. One of the meteoric fragments of this dispersion, the meteorite called ``El Chaco'', consisting of 37 tons, is the second heaviest in the world. These meteorites are of great importance to the worldview of the Moqoit, aboriginal people that inhabit this region. For the local Creole population the meteorites are also relevant, that's why they have being cited in numerous documents and reports since the colonial period. During the first months of 2012, two Argentine artists and the Artistic Director of the German contemporary art exhibition called dOCUMENTA (13) tried to move ``El Chaco'' meteorite to Germany in order to exhibit it as an artistic object. Due to the fact that moving the meteorite could have a negative impact according to the Moqoit cosmology and that they were not able to participate in the decision they begun a manifestation against the movement of El Chaco. The opposition made by aboriginal communities and experts in cultural astronomy was able to stop the transfer. The whole process and its impact on the local community have promoted a deep discussion about art, science and cultural colonialism. In this paper we aim to address this debate and its consequences. This will allow us to think about contemporary forms of colonialism that are hidden in many scientific and artistic projects. Furthermore, we aim to debate about the most effective ways of protecting astronomical heritage in the Third World.

López, Alejandro Martín; Altman, Agustina

2012-09-01

326

L’evolució del pensament escolar al Principat (1808-1845)  

Microsoft Academic Search

L’article analitza la implantació i el desenvolupament de l’ educació escolar a les primeres dècades del segle XIX a Catalunya, concretament des de l’arribada de les tropes napoleòniques el 1808 fins a la consolidació de la reforma liberal de 1845. S’hi realitza una anàlisi cronològica dels esdeveniments polítics, legals i culturals més importants, i s’aprofundeix en el pensament escolar d’aquesta

Jordi Monés i Pujol-Busquets

2007-01-01

327

NCI ofrece apoyo a quienes están necesitados después del huracane Katrina  

Cancer.gov

El Instituto Nacional del Cáncer (NCI), parte de los Institutos Nacionales de la Salud (NIH), está empeñado en ayudar a las miles de personas que han sido asoladas por los efectos del huracán Katrina. NCI está tratando de llegar a los públicos diversos para proporcionarles información relacionada con el cáncer que pueda ser compartida con exactitud y efectividad con quienes han sido afectados por el huracán.

328

CHEK2 1100delC mutation is frequent among Russian breast cancer patients  

Microsoft Academic Search

This study was aimed to assess the role of CHEK2 1100delC mutation in breast cancer (BC) predisposition in Russia. The 1100delC allele was detected in 14\\/660 (2.1%) unilateral BC cases and in 8\\/155 (5.2%) patients with the bilateral form of the disease, but only in 1\\/448 (0.2%) middle-aged control females and in none of 373 elderly tumor-free women. The obtained

Elena V. Chekmariova; Anna P. Sokolenko; Konstantin G. Buslov; Aglaya G. Iyevleva; Yulia M. Ulibina; Maxim E. Rozanov; Natalia V. Mitiushkina; Alexandr V. Togo; Dmitry E. Matsko; Dmitry A. Voskresenskiy; Oleg L. Chagunava; Peter Devilee; Cees Cornelisse; Vladimir F. Semiglazov; Evgeny N. Imyanitov

2006-01-01

329

La ceràmica de procedència sud-itàlica del Museu Episcopal de Vic  

Microsoft Academic Search

En aquest article s’estudien tretze vasos ceràmics procedents del sud d’Itàlia que formen part de la col·lecció de ceràmiques gregues, etrusques, falisques i sud-itàliques del Museu Episcopal de Vic (MEV). Es tracta d’un conjunt homogeni pel que fa a l’origen geogràfic, ja que tots els exemplars provenen de l’Apúlia preromana, avui regió de la Pulla. Els vasos abasten un ventall

2005-01-01

330

Aplicación del método SAC a líneas de Fe II en atmósferas extendidas de estrellas Be  

NASA Astrophysics Data System (ADS)

En el presente trabajo hemos analizado las líneas de Fe II en emisión, para varios multipletes en el rango óptico, presentes en los espectros de un grupo de estrellas Be. Mediante la aplicación del método de curvas de autoabsorción (SAC), obtenemos una estimación del valor de los parámetros característicos de la región en la cual se forman dichas líneas, tales como temperatura de excitación, opacidad y dimensión.

Arias, M. L.; Zorec, J.; Cidale, L.; Morrell, N.; Friedjung, M.; Chauville, J.; Ballereau, D.

331

CÁLCULO DEL VOLUMEN ESPECÍFICO DE LÍQUIDOS PUROS CON ECUACIONES DE ESTADO CÚBICAS  

Microsoft Academic Search

RESUMEN. En este trabajo se presenta una revisión de los cambios que se han producido en los últimos años en las ecuaciones de estado cúbicas de más amplia aceptación y se hace un estudio comparativo del cálculo del volumen específico de once líquidos puros por medio de cinco ecuaciones cúbicas: Van der Waals, Redlich-Kwong, Soave-Redlich-Kwong, Peng-Robinson y Valderrama-Patel- Teja .

BIBIAN HOYOS

332

Pedro García Faria, ingeniero de Caminos (y arquitecto)  

Microsoft Academic Search

Pedro García Faria fue uno de los Ingenieros de Caminos, Canales y Puertos más destacados al servicio de la administración del Estado en España desde finales del siglo XIX hasta principios del XX. El interés que desde los inicios de su carrera profesional mostró por el problema del saneamiento de zonas afectadas por epidemias, le condujeron al reconocimiento profesional de

Miguel Ángel Miranda González

2007-01-01

333

Breast tumors from CHEK2 1100delC-mutation carriers: genomic landscape and clinical implications  

PubMed Central

Introduction Checkpoint kinase 2 (CHEK2) is a moderate penetrance breast cancer risk gene, whose truncating mutation 1100delC increases the risk about twofold. We investigated gene copy-number aberrations and gene-expression profiles that are typical for breast tumors of CHEK2 1100delC-mutation carriers. Methods In total, 126 breast tumor tissue specimens including 32 samples from patients carrying CHEK2 1100delC were studied in array-comparative genomic hybridization (aCGH) and gene-expression (GEX) experiments. After dimensionality reduction with CGHregions R package, CHEK2 1100delC-associated regions in the aCGH data were detected by the Wilcoxon rank-sum test. The linear model was fitted to GEX data with R package limma. Genes whose expression levels were associated with CHEK2 1100delC mutation were detected by the bayesian method. Results We discovered four lost and three gained CHEK2 1100delC-related loci. These include losses of 1p13.3-31.3, 8p21.1-2, 8p23.1-2, and 17p12-13.1 as well as gains of 12q13.11-3, 16p13.3, and 19p13.3. Twenty-eight genes located on these regions showed differential expression between CHEK2 1100delC and other tumors, nominating them as candidates for CHEK2 1100delC-associated tumor-progression drivers. These included CLCA1 on 1p22 as well as CALCOCO1, SBEM, and LRP1 on 12q13. Altogether, 188 genes were differentially expressed between CHEK2 1100delC and other tumors. Of these, 144 had elevated and 44, reduced expression levels. Our results suggest the WNT pathway as a driver of tumorigenesis in breast tumors of CHEK2 1100delC-mutation carriers and a role for the olfactory receptor protein family in cancer progression. Differences in the expression of the 188 CHEK2 1100delC-associated genes divided breast tumor samples from three independent datasets into two groups that differed in their relapse-free survival time. Conclusions We have shown that copy-number aberrations of certain genomic regions are associated with CHEK2 mutation 1100delC. On these regions, we identified potential drivers of CHEK2 1100delC-associated tumorigenesis, whose role in cancer progression is worth investigating. Furthermore, poorer survival related to the CHEK2 1100delC gene-expression signature highlights pathways that are likely to have a role in the development of metastatic disease in carriers of the CHEK2 1100delC mutation.

2011-01-01

334

PROPUESTAS DE ACTIVIDADES RECREATIVAS FÍSICAS PARA ELEVAR EL NIVEL DE PARTICIPACIÓN DE LOS JÓVENES DEL CDR # 4 RENÉ ALMANZA DEL MUNICIPIO AMANCIO  

Microsoft Academic Search

El presente trabajo investigativo realizado en la comunidad René Almanza Soto, del consejo popular 01 en Amancio, constituyó una gran experiencia ya que el objeto de la recreación física son indudablemente los gustos y preferencias recreativas de la población, a través de este método logramos incorporar gran parte de los habitantes a la realización de las actividades físicas recreativas que

Rosalba Mensoney Liben; Gerardo Sutherland Lemes

2010-01-01

335

LAS FLECHAS EN EL ARTE LEVANTINO: APORTACIONES DESDE EL ANÁLISIS DE LOS PROYECTILES DEL REGISTRO ARQUEOLÓGICO DEL RIU DE LES COVES (ALT MAESTRAT, CASTELLÓ )  

Microsoft Academic Search

RESUMEN: En el presente trabajo se aborda el estudio de las flechas en el Arte Levantino a partir de su contrastación con los proyectiles en piedra tallada recuperados en los yacimientos meso- líticos y neolíticos más próximos a los abrigos pintados del núcleo de la Valltorta-Gasulla. El aná- lisis de las diferentes modalidades de representación y las distintas propuestas de

Javier FERNÁNDEZ LÓPEZ DE PABLO

336

Valoración Económica del Manejo Sustentable de la Calidad del Aire y de la Contaminación: Ejemplos de Experiencias, Implicaciones Políticas y Aplicabilidad en el Contexto de la Región  

Microsoft Academic Search

Esta presentación fue comisionada por la Red de Medio Ambiente del Diálogo Regional de Política para la III Reunión Hemisférica celebrada los días 9 y 10 de marzo de 2004. Las ciudades de Latinoamérica tienen niveles altos de contaminación atmosférica, principalmente de material particulado. No hay duda de que la contaminación atmosférica produce efectos nocivos a altas concentraciones, pero la

Luis A. Cifuentes

2004-01-01

337

L'assimilació dels historicismes en l'arquitectura domèstica del segle XIX. De la dependència formal a la consecució d'un estil propi  

Microsoft Academic Search

Aquí se pretende analizar la influencia de los diferentes historicismos en la arquitectura doméstica del siglo XIX a través de las diferentes capitales europeas. Así, se observa cómo la influencia de los estilos históricos, tan importante en la arquitectura oficial y representativa, se limita al uso de algunos elementos estilísticos concretos, pero sin llegar nunca a reproducciones completas de edificios

Joan Molet i Petit

2001-01-01

338

Identification of novel mutations in the ABCA12 gene, c.1857delA and c.5653-5655delTAT, causing harlequin ichthyosis.  

PubMed

Harlequin ichthyosis (HI) is a severe autosomal recessive developmental disorder of the skin that is frequently but not always fatal in the first few days of life. In HI, mutations in both ABCA12 gene alleles must have a severe impact on protein function and most mutations are truncating. The presence of at least one nontruncating mutation (predicting a residual protein function) usually causes a less severe congenital ichthyosis (lamellar ichthyosis or congenital ichthyosiform erythroderma). Here we report on a girl with severe HI diagnosed by prenatal ultrasound at 33 5/7week gestation. Ultrasound findings included ectropion, eclabium, deformed nose, hands and feet, joint contractures, hyperechogenic amniotic fluid and polyhydramnion. After birth, palliative treatment was provided and she died on her first day of life. Sequence analysis of the ABCA12 gene identified two novel mutations, c.1857delA (predicting p.Lys619*) in exon 15 and c.5653-5655delTAT (predicting p.1885delTyr) in exon 37, each in heterozygous state. The c.5653-5655delTAT mutation is not truncating, but the deleted tyrosine at position 1885 is perfectly conserved among vertebrates and molecular studies evaluated the mutation as probably disease causing and damaging. PMID:24055722

Follmann, Johannes; Macchiella, Doris; Whybra, Catharina; Mildenberger, Eva; Poarangan, Cornelia; Zechner, Ulrich; Bartsch, Oliver

2013-09-20

339

Captura y Reconfiguración Cooptada del Estado en Guatemala, México y Colombia Análisis conceptual de las memorias de la Primera Discusión Internacional Sobre Captura y Reconfiguración Cooptada del Estado  

Microsoft Academic Search

La evidencia muestra que el narcotráfico es importante en el fortalecimiento de la corrupción de Guatemala, México y Colombia; por lo tanto, es problemático analizar de modo separado ambos fenómenos. Los hechos muestran también que las organizaciones narcotraficantes de los tres países han aprendido a regular la coerción del Estado. Este aprendizaje ha representado un salto que va desde la

Luis Jorge Garay-Salamanca; Isaac de León-Beltrán; Eduardo Salcedo-Albarán

2010-01-01

340

La red exterior de Puertos del Estado: Información oceanográfica en tiempo real. Puertos del Estado deep water network: Oceanographic information in real time  

Microsoft Academic Search

SUMMARY In the last 20 years, Puertos del Estado has settled a complex marine monitoring system that allows the access to real time oceanographic and meteorological information. One of the networks in this system is the deep water network with buoys deployed at open sea. The access to this information is completely free through the web pages where users can

Marta Alfonso; José Damián López; Isabel Ruiz; Enrique Álvarez; Nuevas Tecnologías

341

Bidimensional spectroscopy of HR Del shell with GMOS-IFU  

NASA Astrophysics Data System (ADS)

The slow and bright classical nova HR Del 1967 was observed in the optical using GEMINI-NORTH GMOS-IFU in 2002 with a spectral range from 0.4 ?m to 1 ?m, a resolution of ˜ 300 km/s, and spatial resolution of ˜ 0''.5. Line intensity maps were obtained for H? +[NII], [OIII] 5007, H? and helium lines. The shell showed a bipolar and clumped structure resembling the shell observed in 1998 (Harman and O'Brien 2003). The [OIII] 5007 map showed seven bright knots. Models of the central source, shell and knots spectra were performed using CLOUDY 94 (Ferland et al. 1998) and RA3D (Diaz 2001). Central source spectra were best fitted with a T_{eff} = 60000 K, log(g) = 6 and log(L_*) = 36.6 Rauch atmosphere model. Shell H-density law was modeled using a power law with ? parameter -1.2 and mass = 4.1 × 10^{-4} M_?. The knots spectra were fitted as gaussian condensations with ˜ 50000cm^{-3} peak H-density and ˜ 20000cm^{-3} average H-density. HeII 4686 emission was found in the knots. The HeII origin is atributted to projected emission from diffuse matter in front of the knots, hypothesis corroborated by about the same HeII intensity observed outside the knots. Physical parameters of the condensations were obtained from our analysis and helium abundances were recalculated. A peak density contrast N/N_S = 270 was found when compared to the medium outside the knots. Electron temperatures ˜ 9500 K, electron densities ˜ 20000cm^{-3} and helium number abundance ˜ 0.2 were derived. Our results showed a constant helium abundance within a narrow radius range which is consistent with impulsive ejection (Kato and Hachisu 1994).

Augusto, A.; Diaz, M.

2006-06-01

342

Predicting Nonspecific Ion Binding Using DelPhi  

PubMed Central

Ions are an important component of the cell and affect the corresponding biological macromolecules either via direct binding or as a screening ion cloud. Although some ion binding is highly specific and frequently associated with the function of the macromolecule, other ions bind to the protein surface nonspecifically, presumably because the electrostatic attraction is strong enough to immobilize them. Here, we test such a scenario and demonstrate that experimentally identified surface-bound ions are located at a potential that facilitates binding, which indicates that the major driving force is the electrostatics. Without taking into consideration geometrical factors and structural fluctuations, we show that ions tend to be bound onto the protein surface at positions with strong potential but with polarity opposite to that of the ion. This observation is used to develop a method that uses a DelPhi-calculated potential map in conjunction with an in-house-developed clustering algorithm to predict nonspecific ion-binding sites. Although this approach distinguishes only the polarity of the ions, and not their chemical nature, it can predict nonspecific binding of positively or negatively charged ions with acceptable accuracy. One can use the predictions in the Poisson-Boltzmann approach by placing explicit ions in the predicted positions, which in turn will reduce the magnitude of the local potential and extend the limits of the Poisson-Boltzmann equation. In addition, one can use this approach to place the desired number of ions before conducting molecular-dynamics simulations to neutralize the net charge of the protein, because it was shown to perform better than standard screened Coulomb canned routines, or to predict ion-binding sites in proteins. This latter is especially true for proteins that are involved in ion transport, because such ions are loosely bound and very difficult to detect experimentally.

Petukh, Marharyta; Zhenirovskyy, Maxim; Li, Chuan; Li, Lin; Wang, Lin; Alexov, Emil

2012-01-01

343

Three primary malignancies related to BRCA mutation successively occurring in a BRCA1 185delAG mutation carrier  

Microsoft Academic Search

The 185delAG and 5382insC mutations in the BRCA1 gene and the 6174delT mutation in the BRCA2 gene (the Ashkenazi mutations) have been found to be significantly more common among Jews of eastern European ancestry (1 in 40, 2.5%) in comparison to the general population (1 in 800 to 1 in 300, 0.12–0.33%). Carriers of these mutations, especially the BRCA1 185delAG

Benjamin Piura; Alex Rabinovich; Ilana Yanai-Inbar

2001-01-01

344

Age of Cu(Fe)Au mineralization and thermal evolution of the Punta del Cobre district, Chile  

Microsoft Academic Search

The Punta del Cobre belt is located 15?km south of Copiap?, northern Chile. It comprises several Cu(-Fe)-Au deposits in the\\u000a Punta del Cobre and Ladrillos districts, east of the Copiap? river, and the Ojancos Nuevo district, with the new Candelaria\\u000a mine, and Las Pintadas district, west of the river. The mineralization in the Punta del Cobre belt is characterized by

R. Marschik; B. S. Singer; F. Munizaga; C. Tassinari; R. Moritz; L. Fontboté

1997-01-01

345

Evolució recent del paisatge agrari de l'Alt Empordà a través de la teledetecció i d'índexs paisatgístics  

Microsoft Academic Search

En l'article es presenta el mètode emprat i els resultats obtinguts en l'evolució de les cobertes i usos del sòl extretes d'imatges satèl·lit de vint-i-un municipis de l'Alt Empordà. En concret s'ha quantificat com han evolucionat des del 1977 al 1997 pel que fa a la fragmentació o compactació a través de diversos índexs emprats en l'Ecologia del Paisatge. Els

Xavier Pons i Fernández; Pere Serra; David Saurí i Pujol

2005-01-01

346

The RGD finger of Del-1 is a unique structural feature critical for integrin binding  

SciTech Connect

Developmental endothelial cell locus-1 (Del-1) glycoprotein is secreted by endothelial cells and a subset of macrophages. Del-1 plays a regulatory role in vascular remodeling and functions in innate immunity through interaction with integrin {alpha}{sub V}{beta}{sub 3}. Del-1 contains 3 epidermal growth factor (EGF)-like repeats and 2 discoidin-like domains. An Arg-Gly-Asp (RGD) motif in the second EGF domain (EGF2) mediates adhesion by endothelial cells and phagocytes. We report the crystal structure of its 3 EGF domains. The RGD motif of EGF2 forms a type II' {beta} turn at the tip of a long protruding loop, dubbed the RGD finger. Whereas EGF2 and EGF3 constitute a rigid rod via an interdomain calcium ion binding site, the long linker between EGF1 and EGF2 lends considerable flexibility to EGF1. Two unique O-linked glycans and 1 N-linked glycan locate to the opposite side of EGF2 from the RGD motif. These structural features favor integrin binding of the RGD finger. Mutagenesis data confirm the importance of having the RGD motif at the tip of the RGD finger. A database search for EGF domain sequences shows that this RGD finger is likely an evolutionary insertion and unique to the EGF domain of Del-1 and its homologue milk fat globule-EGF 8. The RGD finger of Del-1 is a unique structural feature critical for integrin binding.

Schürpf, Thomas; Chen, Qiang; Liu, Jin-huan; Wang, Rui; Springer, Timothy A.; Wang, Jia-huai (Harvard-Med)

2012-11-13

347

Basis for a streamflow forecasting system to Rincón del Bonete and Salto Grande (Uruguay)  

NASA Astrophysics Data System (ADS)

This paper presents the basis for the design of streamflow prediction systems for the hydroelectric dams of Rincón del Bonete (Uruguay) and Salto Grande (Uruguay-Argentina). The prediction is made, independently, for each reservoir and each month of the year with two methodologies: data-driven statistical models and hybrid downscaling that includes atmospheric predictors. We determine a set of potential predictors and then fit linear models coupled with variable selection techniques, under the hypothesis of perfectly known predictors. The predictive skill of the schemes outperforms the climatological forecast throughout the year in both reservoirs (except August in Rincón del Bonete). This remains the case even when the forecast lead does not allow for the use of preceding flows as predictors. While in Rincón del Bonete it is not possible to distinguish a period of high predictability, in Salto Grande, there is a robust signal in March-May and October-December.

Talento, Stefanie; Terra, Rafael

2013-10-01

348

Basis for a streamflow forecasting system to Rincón del Bonete and Salto Grande (Uruguay)  

NASA Astrophysics Data System (ADS)

This paper presents the basis for the design of streamflow prediction systems for the hydroelectric dams of Rincón del Bonete (Uruguay) and Salto Grande (Uruguay-Argentina). The prediction is made, independently, for each reservoir and each month of the year with two methodologies: data-driven statistical models and hybrid downscaling that includes atmospheric predictors. We determine a set of potential predictors and then fit linear models coupled with variable selection techniques, under the hypothesis of perfectly known predictors. The predictive skill of the schemes outperforms the climatological forecast throughout the year in both reservoirs (except August in Rincón del Bonete). This remains the case even when the forecast lead does not allow for the use of preceding flows as predictors. While in Rincón del Bonete it is not possible to distinguish a period of high predictability, in Salto Grande, there is a robust signal in March-May and October-December.

Talento, Stefanie; Terra, Rafael

2013-01-01

349

Observaciones del disco solar y de una protuberancia quiescente en radiación ultravioleta  

NASA Astrophysics Data System (ADS)

Observaciones del disco solar y de una protuberancia quiescente en el rango de longitudes de onda ultravioleta fueron obtenidas con el instrumento CDS (Coronal Diagnostic Spectrograph) y SUMER (Solar Ultraviolet Measurements of emitted radiation) a bordo de la sonda SOHO. El propósito es investigar las velocidades macroscópicas de varias especies metálicas que se observan tanto en el disco solar como en el plasma de las protuberancias. Para calcular las velocidades del disco solar aplicamos una técnica mixta para modelar la distribución de estructuras en UV en el Sol quieto. Las velocidades macroscópicas en las protuberancias se calcularon a partir de los corrimientos Doppler en cada línea espectral y luego se tomaron las del disco solar como referencia. Obtuvimos valores absolutos para las velocidades macroscópicas entre 5 y 40 km/seg. También detectamos comportamientos diferentes en las velocidades de las protuberancias en el centro con respecto a los bordes.

Cirigliano, D.; Vial, J.-C.; Rovira, M.

350

Integrated global background monitoring network. Preliminary results from Torres del Paine and Olympic National Parks  

SciTech Connect

During 1984, a pilot project was initiated for monitoring pollution at Torres del Paine National Park in southern Chile and Olympic National Park in the United States. These are two of three initial sites that are to be established as part of an integrated global backgound monitoring network. Eventually, the plan is to establish a world-wide system of such sites. We collected and analyzed samples of the soil, water, air, and two species of plants (moss and lichen). We also collected and analyzed samples of the forest litter. We compared the samples of soil and vegetation against reference samples. We also compared samples of soil, vegetation, and of organic material from Torres del Paine against similar samples from Olympic and Sequoia-Kings Canyon National Parks in the United States. Although the data is preliminary, it is in agreement with out initial hypothesis that Torres del Paine and Olympic National Parks are not a polluted sites.

Wiersma, G.B.; Kohler, A.; Boelcke, C.; Baker, G.; Harmon, M.; Weber, C.; Gonzales, J.

1985-10-01

351

Trichomycetes living in the guts of aquatic insects of Misiones and Tierra del Fuego, Argentina.  

PubMed

Fourteen species of Trichomycetes living in the guts of aquatic insects are reported from two provinces of Argentina, Misiones and Tierra del Fuego. Twelve of the species belong to the Harpellales and two are Amoebidiales. Five harpellid species are reported from Misiones in the extreme northeast of the country (Genistellospora homothallica, Harpella tica, Smittium culisetae, Smittium sp., Stachylina sp.) and seven are from Tierra del Fuego, the southern tip of South America (H. meridianalis, Glotzia sp., S. culicis, S. cellaspora, S. imitatum, Stachylina minima, Penella simulii). Insect hosts all were immature stages of Culicidae, Simuliidae, Chironomidae, Ceratopogonidae (Insecta: Diptera), and Ephemeroptera and Plecoptera. The lower diversity of Trichomycetes found at Misiones, which has a subtropical climate and rainforest vegetation, was due possibly to the warmer temperatures of the water (15-24 C), compared to the colder streams of Tierra del Fuego (9-15 C), with forests and steppes as typical vegetation. PMID:16396339

López Lastra, Claudia C; Scorsetti, Ana C; Marti, Gerardo A; Coscarón, Sixto

352

DelsGate: a robust and rapid method for gene deletion.  

PubMed

Gene deletion is one of the most powerful tools to study gene function. In the genomics era there is great demand for fast, simple high-throughput methods for gene deletion to study the roles of the large numbers of genes that are being identified. Here we present an approach that speeds up the process of generation of deletion mutants by greatly simplifying the production of gene deletion constructs. With this purpose we have developed a method, which we named DelsGate (Deletion via Gateway), that combines PCR and Gateway cloning technology together with the use of the I-SceI homing endonuclease to generate precise deletion constructs in a very simple, universal and robust manner in just 2 days. DelsGate consists of standard PCR of only the 5' and 3' 1 kb gene flanks directly followed by in vitro Gateway cloning and final generation of the circular deletion construct by in vivo recombination in Escherichia coli. For use in DelsGate we have modified a Gateway cloning vector to include selectable markers for the transformation of Ascomycetes and the Basidiomycete fungus Ustilago maydis. The PCR and transformation steps of DelsGate should be well suited for high-throughput approaches to gene deletion construction in fungal species. We describe here the entire process, from the generation of the deletion construct with DelsGate to the analysis of the fungal transformants to test for gene replacement, with the Basidiomycete fungus Ustilago maydis. Application of DelsGate to other fungal species is also underway. Additionally, we describe how this basic approach can be adapted to other genetic manipulations with minor changes. We specifically describe its application to create unmarked deletions in Ralstonia solanacearum, a Gram-negative phytopathogenic bacterium. PMID:20238261

García-Pedrajas, María D; Nadal, Marina; Denny, Timothy; Baeza-Montañez, Lourdes; Paz, Zahi; Gold, Scott E

2010-01-01

353

Haplotype analysis of the 185delAG BRCA1 mutation in ethnically diverse populations.  

PubMed

The 185delAG* BRCA1 mutation is encountered primarily in Jewish Ashkenazi and Iraqi individuals, and sporadically in non-Jews. Previous studies estimated that this is a founder mutation in Jewish mutation carriers that arose before the dispersion of Jews in the Diaspora ~2500 years ago. The aim of this study was to assess the haplotype in ethnically diverse 185delAG* BRCA1 mutation carriers, and to estimate the age at which the mutation arose. Ethnically diverse Jewish and non-Jewish 185delAG*BRCA1 mutation carriers and their relatives were genotyped using 15 microsatellite markers and three SNPs spanning 12.5?MB, encompassing the BRCA1 gene locus. Estimation of mutation age was based on a subset of 11 markers spanning a region of ~5?MB, using a previously developed algorithm applying the maximum likelihood method. Overall, 188 participants (154 carriers and 34 noncarriers) from 115 families were included: Ashkenazi, Iraq, Kuchin-Indians, Syria, Turkey, Iran, Tunisia, Bulgaria, non-Jewish English, non-Jewish Malaysian, and Hispanics. Haplotype analysis indicated that the 185delAG mutation arose 750-1500 years ago. In Ashkenazim, it is a founder mutation that arose 61 generations ago, and with a small group of founder mutations was introduced into the Hispanic population (conversos) ~650 years ago, and into the Iraqi-Jewish community ~450 years ago. The 185delAG mutation in the non-Jewish populations in Malaysia and the UK arose at least twice independently. We conclude that the 185delAG* BRCA1 mutation resides on a common haplotype among Ashkenazi Jews, and arose about 61 generations ago and arose independently at least twice in non-Jews. PMID:22763381

Laitman, Yael; Feng, Bing-Jian; Zamir, Itay M; Weitzel, Jeffrey N; Duncan, Paul; Port, Danielle; Thirthagiri, Eswary; Teo, Soo-Hwang; Evans, Gareth; Latif, Ayse; Newman, William G; Gershoni-Baruch, Ruth; Zidan, Jamal; Shimon-Paluch, Shani; Goldgar, David; Friedman, Eitan

2012-07-04

354

Jasmin i Frederic Mistral, dos models literaris per a Miquel dels Sants Oliver  

Microsoft Academic Search

M. S. Oliver l'any 1903 va publicar al Diario de Barcelona la secció «Literatura extrangera\\u000a», dedicada a l'anàlisi de l'obra d'alguns dels escriptors europeus més notables de l'època.\\u000aLa posició del crític, basada tant en criteris estètics com morals i polítics, s'expressava amb\\u000auna nitidesa absoluta. D'una banda, rebutjava tres tipus de literatura que aleshores tenien un\\u000agran predicament:

Damià Pons i Pons

2007-01-01

355

Gonadal dysgenesis, Turner syndrome with 46,XX,del(18p)3  

SciTech Connect

The authors report a case of a female infant with gonadal dysgenesis, clinical features of Turner syndrome and a de novo del(18p). The factors controlling gonadal dysgenesis and Turner syndrome are unknown to date. The genes involved could be located not only on X chromosome but also on autosomes. The present case suggests that one of these genes is situated on the short arm of chromosome 18. We conclude that patients with del(18p) syndrome should be evaluated for gonadal dysgenesis.

Telvi, L.; Ion, R. [Hopital Saint Vincent de Paul, Paris (France); Bernheim, A. [CNRS, Villejif (France)] [and others

1994-09-01

356

Cámara CCD Directa con el Telescopio de 2.15 m del CASLEO: algunos diagnósticos  

NASA Astrophysics Data System (ADS)

Se efectuaron algunas pruebas con la cámara CCD (+ Reductor Focal) instalada en el foco Cassegrain del Telescopio de 2.15 m del Complejo Astronómico El Leoncito (CASLEO). Las conclusiones más significativas son: Los tiempos de exposición efectivos difieren de los nominales en una fracción apreciable de segundo. En exposiciones de menos de 3 segundos, la iluminación no es pareja en todo el detector. En consecuencia, se recomiendan los pasos a seguir por los astrónomos tanto durante la observación como en la reducción de sus datos.

Cellone, S. A.

357

Aplicación del Teorema de Nekhorochev para tiempos de estabilidad en Mecánica Celeste  

NASA Astrophysics Data System (ADS)

En Mecánica Celeste, uno de los problemas centrales consiste en la determinación de los tiempos de estabilidad. El teorema de Nekhorochev proporciona un método para dicho estudio, para un sistema determinado por un hamiltoniano descripto en las variables acción-ángulo. El trabajo consiste en la acotación tanto del potencial perturbador y de la matriz hessiana del hamiltoniano integrable para determinar luego el tiempo de estabilidad de dicho sistema, donde por estabilidad se entiende la separación en norma infinito en el espacio de las acciones.

Miloni, O.; Núñez, J.; Brunini, A.

358

A review on Balanites aegyptiaca Del (desert date): phytochemical constituents, traditional uses, and pharmacological activity  

PubMed Central

Balanites aegyptiaca Del. (Zygophyllaceae), known as ‘desert date,’ is spiny shrub or tree up to l0 m tall, widely distributed in dry land areas of Africa and South Asia. It is traditionally used in treatment of various ailments i.e. jaundice, intestinal worm infection, wounds, malaria, syphilis, epilepsy, dysentery, constipation, diarrhea, hemorrhoid, stomach aches, asthma, and fever. It contains protein, lipid, carbohydrate, alkaloid, saponin, flavonoid, and organic acid. Present review summarizes the traditional claims, phytochemistry, and pharmacology of B. aegyptiaca Del reported in scientific literature.

Chothani, Daya L.; Vaghasiya, H. U.

2011-01-01

359

Do crustal deformations observed by GPS in Tierra del Fuego (Argentina) reflect glacial-isostatic adjustment?  

NASA Astrophysics Data System (ADS)

Vertical site velocities determined by geodetic GPS observations in the Lago Fagnano area, Tierra del Fuego main island, are interpreted with respect to their potential relation with the glacial-isostatic crustal response to ice mass changes. The spatial pattern of the uplift rates, in combination with the horizontal crustal deformation pattern, point towards a fault-tectonic rather than glacial-isostatic origin of the determined vertical crustal deformations. This implies rather small GIA effects pointing towards relatively small Holocene ice-mass changes in Tierra del Fuego. However, these findings are considered to be preliminary. They should be confirmed by additional observations covering an extended area with GPS sites.

Mendoza, L.; Richter, A.; Hormaechea, J. L.; Perdomo, R.; Del Cogliano, D.; Dietrich, R.; Fritsche, M.

2010-09-01

360

Recepción y extensión de las explicaciones metafísicas de los números imaginarios en la España del siglo XIX  

Microsoft Academic Search

1. Preliminares El problema del rigor en Matemáticas puede afrontarse al menos desde dos puntos de vista. El primero, que encontramos sobre todo en las Matemáticas contemporáneas, procede del explosivo desarrollo de esta ciencia durante el Siglo XIX y se distingue sobre todo porque los matemáticos ejercientes son entrenados en ciertas técnicas que en líneas generales se componen de una

JOSÉ M. PACHECO CASTELAO

361

Entre metrópolis y periferias. Análisis de los movimientos migratorios a partir del censo de población de 1993  

Microsoft Academic Search

El Área de Demografía y Estudios de Población, del Centro de Investigaciones sobre Dinámica Social –CIDS– de la Universidad Externado de Colombia, definió como objetivo de una de sus Líneas estratégicas de investigación el análisis de los procesos migratorios en Colombia. Aunque la migración constituye un elemento central en la determinación del crecimiento, volumen y composición de la población, este

Alejandro González Pulido; Norma Rubiano

362

DEL SENTIMIENTO ESTÉTICO DE LA VIDA Y SU UTILIDAD ABOUT THE AESTHETIC FEELING OF LIFE AND ITS UTILITY  

Microsoft Academic Search

Resumen Se plantea una reflexión desde el darwinismo y el psicoanálisis sobre el sentido del sentimiento estético, así como de algunas características del mismo, concluyendo que el sentimiento estético es un tónico de la voluntad imprescindi- ble para vivir con el malestar inherente al hecho de saber que somos seres para la muerte. Palabras clave: Estética, conciencia de sí mismo,

Fabio Rivas Guerrero

363

Acumulación de tierras, cambio socio-económico y movilidad espacial en los inmigrantes Wolof del bosque de Pata  

Microsoft Academic Search

A través de una migración que han emprendido desde 1978 en el bosque de Pata (sur de Senegal), los Wolof que han venido de Saloum han dado un nuevo giro a su vida. Los beneficios sacados del cultivo de cacahuete, al cual son particularmente apegados, les han permitido hacer varias realizaciones y algunas inversiones. Así, después del refuerzo de medios

Mamady Sidibe

2007-01-01

364

Caracterización de entornos y determinantes de la eficiencia en los sectores del cuero, el calzado y la marroquinería  

Microsoft Academic Search

Este estudio permite caracterizar al sector en Colombia en seis entornos principales e identificar los principales aspectos que marcan la diferenciaentre las empresas de calzado y marroquinería en cuero, ubicadas en el Área Metropolitana del Valle de Aburrá (Amva),1 seleccionadas a partir de una muestra representativa, y comparadas con otras ciudades del país. Adicionalmente, y lo más importante, ayuda a

David Tobón Orozco; Jorge Barrientos Marín

365

Gene expression profiling assigns CHEK2 1100delC breast cancers to the luminal intrinsic subtypes.  

PubMed

CHEK2 1100delC is a moderate-risk cancer susceptibility allele that confers a high breast cancer risk in a polygenic setting. Gene expression profiling of CHEK2 1100delC breast cancers may reveal clues to the nature of the polygenic CHEK2 model and its genes involved. Here, we report global gene expression profiles of a cohort of 155 familial breast cancers, including 26 CHEK2 1100delC mutant tumors. In line with previous work, all CHEK2 1100delC mutant tumors clustered among the hormone receptor-positive breast cancers. In the hormone receptor-positive subset, a 40-gene CHEK2 signature was subsequently defined that significantly associated with CHEK2 1100delC breast cancers. The identification of a CHEK2 gene signature implies an unexpected biological homogeneity among the CHEK2 1100delC breast cancers. In addition, all 26 CHEK2 1100delC tumors classified as luminal intrinsic subtype breast cancers, with 8 luminal A and 18 luminal B tumors. This biological make-up of CHEK2 1100delC breast cancers suggests that a relatively limited number of additional susceptibility alleles are involved in the polygenic CHEK2 model. Identification of these as-yet-unknown susceptibility alleles should be aided by clues from the 40-gene CHEK2 signature. PMID:21614566

Nagel, Jord H A; Peeters, Justine K; Smid, Marcel; Sieuwerts, Anieta M; Wasielewski, Marijke; de Weerd, Vanja; Trapman-Jansen, Anita M A C; van den Ouweland, Ans; Brüggenwirth, Hennie; van I Jcken, Wilfred F J; Klijn, Jan G M; van der Spek, Peter J; Foekens, John A; Martens, John W M; Schutte, Mieke; Meijers-Heijboer, Hanne

2011-05-26

366

L'azione del propranololo sulla glicemia e sull'insulina immunoreattiva in una paziente affetta da insulinoma  

Microsoft Academic Search

Riassunto Con la somministrazione orale di propranololo ad una paziente affetta da insulinoma si è notato un miglioramento delle condizioni cliniche e una minor frequenza delle crisi ipoglicemiche. I valori di glicemia e di IRI si sono portati nella norma. Vengono riportati e brevemente discussi i risultati delle prove del digiuno, del glucagone e della leucina praticate prima e durante

Vittorio Neri; Alberto Bartorelli; Giovanni Faglia

1969-01-01

367

Efectos del desajuste educativo sobre el rendimient o de la escolaridad: Un análisis para el caso español  

Microsoft Academic Search

El objetivo del presente estudio es analizar grado de adecuación entre educación y empleo en el mercado de trabajo español, centrándonos principalmente en el fenómeno de la sobreeducación y estudiando sus e fectos sobre los ingresos individuales. Más concretamente, se aborda el estud io del desajuste educativo desde una doble perspectiva: por una parte, se calculan los r endimientos privados

Inés P. Murillo Huertas; Marta Rahona López; Maria del Mar; Salinas Jiménez

368

OFERTA vs. DEMANDA: ALGUNOS ASPECTOS A CONSIDERAR PARA EL ESTUDIO DEL MERCADO EN SALUD  

Microsoft Academic Search

E n s a y o En este artículo se discuten algunos de los principales aspectos a considerar al momento de abordar el análisis del mercado de los servicios de salud. Las consideraciones conceptuales se refieren tanto a la demanda como a la oferta, aunque el análisis pudiera centrarse en algunos momentos hacia la demanda. Por otra parte, las mismas

Arredondo López; Recaman Mejía

369

La estructura de mercado del sector de las artes escénicas en España  

Microsoft Academic Search

La diversidad de agentes y de comportamientos, así como la complejidad que encierra la existencia e interrelación de dos mercados, el de la producción y el de la difusión, han dificultado el estudio del sistema escénico español desde perspectivas científicas. Con el objetivo de cubrir parcialmente ese vacío y presentar las especificidades de dicho sistema, el presente trabajo utiliza el

LLUÍS BONET; ANNA VILLARROYA

2009-01-01

370

LA RAZA COMO DETERMINANTE DEL ACCESO A UN EMPLEO DE CALIDAD: UN ESTUDIO PARA CALI  

Microsoft Academic Search

La discriminación laboral por raza ha sido un tema ampliamente analizado en la economía del trabajo a partir de la estimación de ecuaciones de ingreso para determinar diferenciales en el salario, pero la influencia de este factor en el acceso a un empleo de calidad no se ha explorado lo suficiente. Realizar este análisis cobra vital importancia para una ciudad

CHRISTIAN DAVID BUSTAMANTE; SANTIAGO ARROYO

2008-01-01

371

Las fundaciones de ciudades y el pensamiento urbanístico hispano en la era del Descubrimiento  

Microsoft Academic Search

La historia urbana referida al periodo histórico en torno a la fecha de 1492, con sus precedentes, desde la caída de Constantinopla (1452) y su evolución hasta la entronización y asentamiento de la monarquía del Emperador Carlos (~1530), es un periodo excepcional que requiere una reinterpretación a la luz de nuevos enfoques interdisciplinarios y de nuevos descubrimientos científicos. El periodo

María Isabel Navarro Segura

2007-01-01

372

Estudio Comparado de la Tributación del Sector Transporte de Carga en Argentina, Brasil, y Uruguay  

Microsoft Academic Search

En un contexto de creciente integración internacional, caracterizado por la formación de bloques económicos regionales, el sector del transporte de carga cobra cada vez más prominencia. Por ejemplo, el tratamiento tributario conferido al sector por los distintos países participantes de un bloque crea incentivos diferenciados en relación con la ampliación de la integración comercial.

Erika Amorim Araujo

2005-01-01

373

EL DISCURSO RACIALIZADOR: DISCRIMINACIÓN Y AUTOCTONÍA EN LA ARGENTINA DEL BICENTENARIO  

Microsoft Academic Search

La dinámica del prejuicio y el racismo son fenómenos que no necesariamente deben ser estudiados con grandes y complejas escalas tomando grandes muestras de población o en experimentos de laboratorio controlados. Una perspectiva micro-social y etnográfica precisa de 30 minutos y “no intrusiva” no sólo puede ser suficiente metodológicamente en el estudio de estos problemas sino a veces esclarecedora. El

Maximiliano Korstanje

2009-01-01

374

EL SALVAJE METROPOLITANO: RECONSTRUCCIÓN DEL CONOCIMIENTO SOCIAL EN EL TRABAJO DE CAMPO  

Microsoft Academic Search

El salvaje metropolitano no es sólo un libro de antropología sino además un tratado hermenéutico sobre la filosofía del método que ayuda a los lectores más allá de su disciplina de base a hacer uso de la etnografía en sus trabajos de campo. En base a lo expuesto, Rosana Guber comienza su capítulo introductorio con un análisis sobre las limitaciones

Maximiliano Korstanje

2008-01-01

375

CIENCIA Y NOCIENCIA EN EL TURISMO: RESPUESTA AL ANTROPÓLOGO DEL TURISMO MAXIMILIANO KORSTANJE  

Microsoft Academic Search

He leído con suma atención el trabajo de Korstanje pero tengo que decir, de entrada, que, desafortunadamente, ese trabajo no responde, como su autor cree, al mío. Mi trabajo versa sobre un hecho constatable, no sobre una opinión o sobre una teoría: la ausencia o ignorancia en la literatura del turismo de la función de producción. Como vengo demostrando, el

Francisco Muñoz de Escalona

2010-01-01

376

Cache Valley Virus in the Del Mar VA Peninsula. Ii. Identity of Virus Recovered from Mosquitoes.  

National Technical Information Service (NTIS)

Viruses recovered on the Del Mar Va peninsula from northern salt-marsh mosquitoes Aedes sollicitans and Aedes taeniorhynchus from 1961 through 1964 were shown to be similar to, if not indistinguishable from, Cache Valley virus, and different from Tensaw v...

T. M. Yuill E. L. Buescher

1969-01-01

377

Pedagogia del cos simbòlic. El cos com a valor emergent entre els joves  

Microsoft Academic Search

El artículo aborda desde una perspectiva socio-antropológica el cuerpo humano. Los cambios paradigmáticos sucedidos en disciplinas artísticas así como en el campo de las humanidades permiten una lectura simbólica del cuerpo humano. Es justamente esta dimensión simbólica que nos permite presentarlo como un valor emergente en las identidades de los jóvenes; identidades que pasan por transformar el cuerpo a través

Jordi Planella Ribera

2003-01-01

378

Ouabain Mimics Low Temperature Rescue of F508del-CFTR in Cystic Fibrosis Epithelial Cells  

PubMed Central

Most cases of cystic fibrosis (CF) are caused by the deletion of a single phenylalanine residue at position 508 of the cystic fibrosis transmembrane conductance regulator (CFTR). The mutant F508del-CFTR is retained in the endoplasmic reticulum and degraded, but can be induced by low temperature incubation (29°C) to traffic to the plasma membrane where it functions as a chloride channel. Here we show that, cardiac glycosides, at nanomolar concentrations, can partially correct the trafficking of F508del-CFTR in human CF bronchial epithelial cells (CFBE41o-) and in an F508del-CFTR mouse model. Comparison of the transcriptional profiles obtained with polarized CFBE41o-cells after treatment with ouabain and by low temperature has revealed a striking similarity between the two corrector treatments that is not shared with other correctors. In summary, our study shows a novel function of ouabain and its analogs in the regulation of F508del-CFTR trafficking and suggests that compounds that mimic this low temperature correction of trafficking will provide new avenues for the development of therapeutics for CF.

Zhang, Donglei; Ciciriello, Fabiana; Anjos, Suzana M.; Carissimo, Annamaria; Liao, Jie; Carlile, Graeme W.; Balghi, Haouaria; Robert, Renaud; Luini, Alberto; Hanrahan, John W.; Thomas, David Y.

2012-01-01

379

Boswellia papyrifera (Del.) Hochst: a tropical key species in northern Ethiopia  

Microsoft Academic Search

Boswellia papyrifera (Del.) Hochst is a dryland tree species native to Ethiopia and widely known for its frankincense production. Besides, it has also other numerous environmental, socio-economic, traditional and industrial benefits. However, the population of the species is declining at an alarming rate due to extensive human encroachment. This paper presents the importance of the species, techniques of tapping, harvesting

Kindeya Gebrehiwot; Bart Muys; Mitiku Haile; Ralph Mitloehner

380

High carrier frequency of the 35delG deafness mutation in European populations  

Microsoft Academic Search

Congenital deafness accounts for about 1 in 1000 infants and approximately 80% of cases are inherited as an autosomal recessive trait. Recently, it has been demonstrated that connexin 26 (GJB2) gene is a major gene for congenital sensorineural deafness. A single mutation (named 35delG) was found in most recessive families and sporadic cases of congenital deafness, among Caucasoids, with relative

Paolo Gasparini; Raquel Rabionet; Guido Barbujani; Salvatore Melchionda; Michael Petersen; Karen Brøndum-Nielsen; Andres Metspalu; Eneli Oitmaa; Marina Pisano; Paolo Fortina; Leopoldo Zelante; Xavier Estivill

2000-01-01

381

Estructura interna y baremación del Test de Pensamiento Creativo de Torrance  

Microsoft Academic Search

Con el presente trabajo se pretende, por una parte, estudiar la estructura interna del TTCT (Torrance Thinking Creative TTCT), y, por otra, establecer los baremos con el fin de poder utilizar el test en el diagnóstico y ambientes educativos. En el trabajo han participado 649 alumnos (319 niñas y 330 ni- ños) cuyas edades oscilan entre 5 y 12 años

Mercedes Ferrando; Carmen Ferrándiz; María Rosario Bermejo; Cristina Sánchez; Joaquín Parra; María Dolores Prieto

2007-01-01

382

Análisis del metabolismo energético y de materiales de Brasil, Chile y Venezuela  

Microsoft Academic Search

El artículo presenta una aplicación de las metodologías de Análisis de Flujo de Materiales y Análisis Integrado Multi- Escala del Metabolismo Social (MSIASM, siglas en inglés) para la caracterización de modelos de desarrollo económico de Brasil, Chile, y Venezuela, mediante la utilización combinada de datos económicos y de uso de energía y materiales para diferentes sectores y en diversos niveles

Nina Eisenmenger; Jesús Ramos Martín; Heinz Schandl

2007-01-01

383

ACTIVIDADES PARA DESARROLLAR LA EXPRESIÓN ORAL Y ESCRITA DEL TÉCNICO MEDIO EN CONTABILIDAD  

Microsoft Academic Search

El siguiente trabajo responde a un problema que enfrenta el C\\/P Osvaldo Socarrás Martínez, relacionado con las insuficiencias que afectan el desarrollo de la Lengua Materna en los estudiantes, por lo que el objetivo de la misma es el de la elaboración de actividades para favorecer la expresión oral y escrita en los estudiantes del segundo año de técnico medio

Orlando Martínez Cuba; Lisandra Ávila Arzuaga

2010-01-01

384

Natural sinks of CO 2 : Technical synthesis from the palmas Del Mar Workshop  

Microsoft Academic Search

Natural CO2 sinks in terrestrial and marine environments are important components of the global carbon cycle, yet the sign and magnitudes of key fluxes among them are unknown. The results of the Palmas Del Mar Workshop — Natural Sinks of CO2 presented in this special issue and its companion hardbound volume of Water, Air, & Soil Pollution, provide a synthesis

John P. Downing; Dominic A. Cataldo

1992-01-01

385

Anti-free radical activities of kaempferol isolated from Acacia nilotica (L.) Willd. Ex. Del  

Microsoft Academic Search

In the present study the polyphenolic compound has been isolated from methanol extract of Acacia nilotica Willd. Ex. Del. which has been identified as kaempferol (AN-5) by NMR and mass spectroscopy. The antioxidant potential of the AN-5 was demonstrated in several in vitro assays: measuring the proton radical scavenging activity (DPPH scavenging assay), hydroxyl radical scavenging activity (deoxyribose degradation assay),

Rajbir Singh; Bikram Singh; Sukhpreet Singh; Neeraj Kumar; Subodh Kumar; Saroj Arora

2008-01-01

386

ATMOSPHERIC VOLATILE ORGANIC COMPOUND MEASUREMENTS DURING THE 1996 PASO DEL NORTE OZONE STUDY  

EPA Science Inventory

Ambient air VOC samples were collected at surface air quality monitoring sites, near sources of interest, and aloft on the US (El Paso) and Mexican (Ciudad Juarez) side of the border during a six-week period of the 1996 Paso del Norte Ozone Study. Samples were collected at five...

387

LA PRIMERA FRASE... Y EL FINAL DEL CUENTO: DOS CUENTOS MARAVILLOSOS DE CARMEN MARTÍN GAITE  

Microsoft Academic Search

An analysis of the structure of two short stories by Carmen Martín Gaite: El Castillo de las Tres Murallas and El Pastel del Diablo (The Devil's Cake). These short stories follow the traditional structure of the quest and the journey, but they also propose an interpretatio n from within that reflects and completes the tale.

Ma VICENTA; HERNÁNDEZ ÁLVAREZ

388

Nutrición deportiva en la infancia: Cumplimiento de las demandas metabólicas del crecimiento y el ejercicio  

Microsoft Academic Search

ExtractoLos objetivos fundamentales de la infancia son el crecimiento y la maduración óptimos. Estos procesos relacionados con el desarrollo exigen un balance energético y nitrogenado positivo. Aunque la actividad física regular puede intensificar el desarrollo en la infancia, un entrenamiento físico extenuante puede reducir las reservas de energía y nitrógeno del organismo que, entonces, deberán reemplazarse a través de la

C. L. Zanker

2006-01-01

389

Antimicrobial activity of flavonoids in medicinal plants from Taf?? del Valle (Tucumán, Argentina)  

Microsoft Academic Search

Preliminary studies of flavonoids have been realised in five native species from Taf?? del Valle (Tucumán, Argentina) used in popular medicine. Most of compounds detected were flavonoids mono and dihydroxylated in B ring. Screening for antimicrobial activity against Gram positive and Gram negative microorganisms has been realised with Lippia turbinata, Satureja parvifolia, Sambucus peruviana, Verbena officinalis and Chenopodium graveolens. The

Nancy E. Hernández; M. L. Tereschuk; L. R. Abdala

2000-01-01

390

AMBIENTE INTEGRATO DI PROGETTAZIONE ASSISTITA DI ROLLERCOASTER: ANALISI DEL COMPORTAMENTO DINAMICO E STRUTTURALE DELLA VETTURA  

Microsoft Academic Search

Sommario In questo lavoro viene illustrato uno strumento di modellazione e simulazione multicorpo di roller coaster che si inserisce in una più ampia procedura di progettazione sviluppata per conto di una delle maggiori aziende del settore. Il codice e la metodologia sviluppati investono l'ideazione, il disegno, la simulazione e la verifica funzionale e dinamica di questa particolare tipologia di giostre

C. Braccesi; F. Cianetti; G. Lori

391

Holocene glaciations in the Ema Glacier valley, Monte Sarmiento Massif, Tierra del Fuego  

Microsoft Academic Search

Investigations carried out in the Ema Glacier valley, Tierra del Fuego, on the eastern side of Monte Sarmiento Massif, enable the recognition of five Holocene glacial events. The oldest glacial advance deposited the so-called external moraines of Ema Glacier, with a probable occurrence between 6000 and 5000 14 C y BP without discarding the potential for these deposits to be from

Jorge Strelin; Gino Casassa; Gunhild Rosqvist; Per Holmlund

2008-01-01

392

Design, Development and Optimization of Highly Efficient Solar Cars: Gato del Sol I-IV  

Microsoft Academic Search

Engineering students at the University of Kentucky have designed and built solar powered cars for the 2008 North American Solar Challenge and the 2009 Formula Sun Grand Prix. Gato del Sol III, our most accomplished car, is powered by a solar array of 480 Silicon monocrystalline photovoltaic cells. Maximum Power Point trackers efficiently made use of these cells and tracked

Raghu Mangu; Krishna Prayaga; Bhavananda Nadimpally; Sam Nicaise

2010-01-01

393

REPRODUCTIVE RHYTHMICITY OF THE ATHERINID FISH, 'COLPICHTHYS REGIS', FROM ESTERO DEL SOLDADO, SONORA, MEXICO  

EPA Science Inventory

The reproductive rhythmicity of the false grunion, Colpichthys regis, was observed in the Estero del Soldado, Mexico during October 1982 through January 1983. Spawning runs occurred at approximately 2-week intervals during daytime high tides. These high tides coincided with new a...

394

Implementation of tsunami disaster prevention measures in the municipality of San Rafael del Sur, Nicaragua  

Microsoft Academic Search

The Nicaraguan Pacific coast presents considerable tsunami risk. On September 1, 1992, a tsunami caused enormous damage in the infrastructure and killed more than 170 people. A pilot project was conducted between 2006 and 2007 in the municipality of San Rafel del Sur, area of Masachapa, The project included multiple topics of tsunami prevention measures and considering the direct participation

W. Strauch; E. Talavera; N. Acosta; M. Sanchez; E. Mejia

2007-01-01

395

Impacto Económico en la Salud por Contaminación del Aire en Lima Metropolitana  

Microsoft Academic Search

I. Objetivos y Justificación del Proyecto: La contaminación ambiental es un problema que se ha venido agravando en las ?ltimas décadas en el Perú, sin embargo, en la práctica es poco lo que se ha hecho para reducirla a niveles aceptables o permitidos. A principios de la presente década se han emitido ciertas normas legales que buscan regular los niveles

Juan José Miranda

396

Las políticas públicas de fomento del reciclaje: La regeneración de aceites usados  

Microsoft Academic Search

Los aceites usados son un residuo peligroso del que pueden derivarse graves daños medioambientales si su gestión es inadecuada; al mismo tiempo, tienen un indudable valor económico, ya que conservan gran parte de los hidrocarburos que los lubricantes contenían originalmente. La gestión de los aceites usados ofrece distintas alternativas, dirigidas principalmente al reciclaje, siendo de destacar la combustión y la

Asunción Arner Güerre; Ramón Barberán Ortí; Jesús Mur Lacambra

2003-01-01

397

Efectos heterogéneos del trabajo infantil en la adquisición de habilidades cognitivas  

Microsoft Academic Search

El estudio se ocupa de los efectos heterogéneos del trabajo en la formación de capital humano a través de una definición amplia de trabajo que incluye toda actividad asociada a responsabilidades no escolares. Dada la variedad de responsabilidades asumidas por niños y adolescentes peruanos, se toma en cuenta dos criterios para clasificar los diferentes tipos de trabajo: el tipo de

Carmen Ponce

2012-01-01

398

Counter-Utopia and Anti-Epic History Writing in Gabriel Miró's El abuelo del rey  

Microsoft Academic Search

In El abuelo del rey (1915) Gabriel Miró tells of the last member of a provincial dynasty, who in the late 1800s travels to the farthest corner of South America expecting to find glory for himself and for his nation. Instead, he ends up working as a petty clerk. In this article, it is argued that through this un-heroic character

Álvaro A. Ayo

2010-01-01

399

Del Bueno Recalls Queso Fresco Casero Cheese Because of Possible Health Risk  

NASA Website

Del Bueno of Grandview, WA is recalling all 16oz. size packages of Queso Fresco Casero Fresh Cheese with a date stamp of Sept 14, 2011 because it has the potential to be contaminated with Listeria monocytogenes, an organism which can cause serious ...

400

LA IMPORTANCIA DE LA ÉTICA EN LA VENTA DESDE EL PUNTO DE VISTA DEL CONSUMIDOR  

Microsoft Academic Search

Este trabajo se centra en el estudio de la ética en la venta, más concretamente en la importancia que los consumidores conceden a la corrección ética del comportamiento de los vendedores con los que entran en contacto en una relación de compraventa. Lo que se pretende conocer es si la actuación ética de los vendedores en la relación de compraventa

M. A. Revilla Camacho; M. A. Gallego Agueda

2007-01-01

401

Dropouts, Stopouts, Optouts at Del Mar College: Spring 1991. Report of a Joint Research Project.  

ERIC Educational Resources Information Center

|A research project investigated 2,313 persons who attended Del Mar College (Texas) during fall 1990 but did not enroll for spring 1991. Four sets of data were collected: (1) limited demographic information on the total student body; (2) demographic information drawn from school records to describe all nonreturnees; (3) demographic information…

Luckie, Jo Ann I.; Bonham, L. Adrianne

402

Principales resultados del Estudio Nacional de Exámenes de pulmón (NLST): Síntesis  

Cancer.gov

El 4 de noviembre de 2010, el Estudio Nacional de Exámenes de Pulmón dio a conocer resultados iniciales que indican que hubo 20% menos muertes por cáncer de pulmón entre los participantes del estudio evaluados con tomografía computarizada espiral de baja dosis (también conocida como tomografía computarizada helicoidal) que en los participantes que fueron evaluados con radiografías de pecho.

403

Plan Multinacional de Educacion del Adulto (Multinational Plan for Adult Education).  

ERIC Educational Resources Information Center

This document contains part of the final report from the first meeting of the Inter-American Council on Education, Science, and Culture, held in Vina del Mar, Chile, in 1970. The report presents general policy and guidelines which should be followed in the establishment of adult elementary education programs. The general discussion covers literacy…

Eduplan Informa, 1971

1971-01-01

404

CONTROLLO SANITARIO DI CONIGLI SELVATICI NEL PARCO DEL SERIO (PROVINCIA DI BERGAMO)  

Microsoft Academic Search

Diagnostic investigation on a wild rabbit population in the Serio Park (Bergamo Province, Northern Italy). During the July - September 1997 period, six hundred wild rabbits (Oryctolagus cuniculus) were shot in the Parco del Serio area (Bergamo province, Northern Italy). This action was decided in order to reduce the number of rabbits, considered too high, and to limit the diffusion

2003-01-01

405

33 CFR 162.200 - Marina del Rey, Calif.; restricted area.  

Code of Federal Regulations, 2013 CFR

...REGULATIONS § 162.200 Marina del Rey, Calif.; restricted area. (a) The area . That portion of the Pacific Ocean lying shoreward of the offshore breakwater and the most seaward 1,000 feet of the entrance channel between the north and...

2013-07-01

406

Biotic association and palaeoenvironmental reconstruction of the “Loma del Pterodaustro” fossil site (Early Cretaceous, Argentina)  

Microsoft Academic Search

A sedimentological analysis of the basal section of the Early Cretaceous, lacustrine LagarcitoFormation at “Loma del Pterodaustro” (San Luis, Argentina) and a summary of its biological components are presented. Three sedimentological facies can be recognized in the basal sequence of the Lagarcito Formation. Fossil remains are particularly abundant in laminated claystones of a facies interpreted as deposits formed in offshore

Luis Chiappe; David Rivarola; Alberto Cione; Marian Fregenal-Martínez; Héctor Sozzi; Luis Buatois; Oscar Gallego; José Laza; Edgardo Romero; Adriana López-Arbarello; Angela Buscalioni; Claudia Marsicano; Susana Adamonis; Francisco Ortega; Sherri McGehee; Osvaldo Di Iorio

1998-01-01

407

Shed Some Light on the Subject: Teaching Ramon del Valle-Inclan's "Luces de bohemia"  

ERIC Educational Resources Information Center

This essay seeks to provide parallel and interchangeable approaches to teaching Ramon del Valle-Inclan's challenging play "Luces de bohemia". A greater understanding of the cultural and mental frameworks of the early twentieth-century Spanish spectator will permit students to penetrate the dense intertextuality that characterizes Valle's…

Parker, Jason Thomas

2011-01-01

408

Rare genotype del2,3\\/2184insA in a cystic fibrosis patient  

Microsoft Academic Search

In this paper we present an interesting case of cystic fibrosis patient with rare genotype del2,3\\/2184insA and atypical clinical image including: mild symptoms in an early phase of disease, quick progress of lung disease, compli- cated with pneumothorax after Bordetella pertussis infection and very good response to systemic and inhaled steroid therapy.

Alina Minarowska; Oksana Kowalczuk; Maciej Kaczmarski; Danuta Kowalczuk

409

Characterization of Nasal Potential Difference in cftr Knockout and F508del-CFTR Mice  

PubMed Central

Background Treatments designed to correct cystic fibrosis transmembrane conductance regulator (CFTR) defects must first be evaluated in preclinical experiments in the mouse model of cystic fibrosis (CF). Mice nasal mucosa mimics the bioelectric defect seen in humans. The use of nasal potential difference (VTE) to assess ionic transport is a powerful test evaluating the restoration of CFTR function. Nasal VTE in CF mice must be well characterized for correct interpretation. Methods We performed VTE measurements in large-scale studies of two mouse models of CF—B6;129 cftr knockout and FVB F508del-CFTR—and their respective wild-type (WT) littermates. We assessed the repeatability of the test for cftr knockout mice and defined cutoff points distinguishing between WT and F508del-CFTR mice. Results We determined the typical VTE values for CF and WT mice and demonstrated the existence of residual CFTR activity in F508del-CFTR mice. We characterized intra-animal variability in B6;129 mice and defined the cutoff points for F508del-CFTR chloride secretion rescue. Hyperpolarization of more than -2.15 mV after perfusion with a low-concentration Cl- solution was considered to indicate a normal response. Conclusions These data will make it possible to interpret changes in nasal VTE in mouse models of CF, in future preclinical studies.

Saussereau, Emilie Lyne; Roussel, Delphine; Diallo, Siradiou; Debarbieux, Laurent; Edelman, Aleksander; Sermet-Gaudelus, Isabelle

2013-01-01

410

Ritrovato per migliorare la qualità della traduzione automatica basato sulla teoria correlazionale del pensiero  

Microsoft Academic Search

In questo articolo l'autore descrive un ritrovato per migliorare la qualità della traduzione automatica i- deato da Silvio Ceccato e basato sulla sua teoria correlazionale del pensiero. Basandosi su esso Ceccato e i suoi collaboratori si dedicarono al problema della traduzione automatica dal 1959 al 1966, compien- do ricerche di tipo quasi esclusivamente teorico. Il progetto fu interrotto quando la

Giulio Benedetti

2005-01-01

411

Integrated global background monitoring network. Preliminary results from Torres del Paine and Olympic National Parks  

Microsoft Academic Search

During 1984, a pilot project was initiated for monitoring pollution at Torres del Paine National Park in southern Chile and Olympic National Park in the United States. These are two of three initial sites that are to be established as part of an integrated global backgound monitoring network. Eventually, the plan is to establish a world-wide system of such sites.

G. B. Wiersma; A. Kohler; C. Boelcke; G. Baker; M. Harmon; C. Weber; J. Gonzales

1985-01-01

412

El modelo de crecimiento hacia adentro: una interpretación del caso colombiano  

Microsoft Academic Search

En este trabajo se realiza un análisis de los fundamentos teóricos de la CEPAL, haciendo énfasis en las ideas que sobre la industrialización diseñó la institución. Se estudia la forma en que se integran las economías latinoamericanas con el resto del mundo y la manera en que las han afectado las crisis de los años treinta y luego la de

Luis E. Vallejo Zamudio

2003-01-01

413

Aerial Radiometric and Magnetic Survey: Del Rio National Topographic Map, Texas. Final Report.  

National Technical Information Service (NTIS)

The results of analyses of the airborne gamma radiation and total magnetic field survey flown for the region identified as the Del Rio National Topographic Map NH14-7 are presented. The airborne data gathered are reduced by ground computer facilities to y...

1980-01-01

414

Genetics of Suicidal Germination of Striga hermonthica (Del.) Benth by Cotton  

Microsoft Academic Search

of the crop and abandonment of otherwise productive fields (Butler, 1995). Well over a decade ago, a conserva- The germination of giant witchweed (Striga hermonthica (Del.) tive estimate of crop losses due to Striga in Africa was Benth), a noxious root parasite of many cereal crops, is stimulated by exudates from the roots of both host and non-host trap plants.

C. J. Botanga; S. O. Alabi; C. A. Echekwu; S. T. O. Lagoke

415

Calidad objetiva y su relación con la formación y la satisfacción del empresario: El caso de los alojamientos rurales españoles1  

Microsoft Academic Search

La revisión de la literatura desvela una relación entre el perfil del pequeño empresario y la competitividad del negocio que regenta. A su vez, observamos la progresiva utilización de estándares de calidad en el sector del turismo rural, bien por parte de la Administración o por asociaciones privadas. El desarrollo de estándares se debe a los resultados empresariales positivos que

Rosa María; Hernández Maestro; Pablo A. Muñoz Gallego; Libia Santos Requejo

416

LA INFLUENCIA DE LA CULTURA Y DEL ESTILO DE GESTIÓN SOBRE EL CLIMA ORGANIZACIONAL. ESTUDIO DE CASO DE LA MEDIANA EMPRESA EN LA DELEGACIÓN IZTAPALAPA  

Microsoft Academic Search

El objetivo del presente documento es mostrar la influencia de la cultura y del perfil cultural, sobre el clima organizacional en las empresas del giro industrial de la Ciudad de México, ubicadas en la Delegación Iztapalapa. Para abordar el estudio de la cultura y el clima organizacional se recurrió al análisis organizacional y al estudio de caso comparativo de medianas

IRENE J. GUILLÉN MONDRAGÓN; ALMA PATRICIA ADUNA MONDRAGÓN

2008-01-01

417

A chemical corrector modifies the channel function of F508del-CFTR.  

PubMed

The deletion of Phe-508 (F508del) constitutes the most prevalent cystic fibrosis-causing mutation. This mutation leads to cystic fibrosis transmembrane conductance regulator (CFTR) misfolding and retention in the endoplasmic reticulum and altered channel activity in mammalian cells. This folding defect can however be partially overcome by growing cells expressing this mutant protein at low (27 degrees C) temperature. Chemical "correctors" have been identified that are also effective in rescuing the biosynthetic defect in F508del-CFTR, thereby permitting its functional expression at the cell surface. The mechanism of action of chemical correctors remains unclear, but it has been suggested that certain correctors [including 4-cyclohexyloxy-2-(1-[4-(4-methoxy-benzenesulfonyl)-piperazin-1-yl]-ethyl)-quinazoline (VRT-325)] may act to promote trafficking by interacting directly with the mutant protein. To test this hypothesis, we assessed the effect of VRT-325 addition on the channel activity of F508del-CFTR after its surface expression had been "rescued" by low temperature. It is noteworthy that short-term pretreatment with VRT-325 [but not with an inactive analog, 4-hydroxy-2-(1-[4-(4-methoxy-benzenesulfonyl)-piperazin-1-yl]-ethyl)-quinazoline (VRT-186)], caused a modest but significant inhibition of cAMP-mediated halide flux. Furthermore, VRT-325 decreased the apparent ATP affinity of purified and reconstituted F508del-CFTR in our ATPase activity assay, an effect that may account for the decrease in channel activity by temperature-rescued F508del-CFTR. These findings suggest that biosynthetic rescue mediated by VRT-325 may be conferred (at least in part) by direct modification of the structure of the mutant protein, leading to a decrease in its ATP-dependent conformational dynamics. Therefore, the challenge for therapy discovery will be the design of small molecules that bind to promote biosynthetic maturation of the major mutant without compromising its activity in vivo. PMID:20501743

Kim Chiaw, Patrick; Wellhauser, Leigh; Huan, Ling Jun; Ramjeesingh, Mohabir; Bear, Christine E

2010-05-25

418

Meta-analysis of CHEK2 1100delC variant and colorectal cancer susceptibility.  

PubMed

Cell cycle checkpoint kinase 2 (CHEK2) gene has been inconsistently associated with colorectal cancer (CRC), particularly the 1100delC variant. To generate large-scale evidence on whether the CHEK2 1100delC variant is associated with CRC susceptibility we have conducted a meta-analysis. Data were collected from the following electronic databases: PubMed, Excerpta Medica Database and Chinese Biomedical Literature Database, with the last report up to November 2010. The odds ratio (OR) and its 95% confidence interval (95% CI) were used to assess the strength of association. We evaluated the contrast of carriers versus non-carriers. Meta-analysis was performed in a fixed/random effect model by using the software Review Manager 4.2. A total of six studies including 4194 cases and 10,010 controls based on the search criteria were involved in this meta-analysis. A significant association of the CHEK2 1100delC variant with unselected CRC was found (OR=2.11, 95% CI=1.41-3.16, P=0.0003). We also found an association of the CHEK2 1100delC variant with familial CRC (OR=2.80, 95% CI=1.74-4.51, P<0.0001). However, the association was not established for sporadic CRC (OR=1.45, 95% CI=0.49-4.30, P=0.50). This meta-analysis demonstrates that the CHEK2 1100delC variant may be an important CRC-predisposing gene, which increases CRC risk. PMID:21807500

Xiang, He-ping; Geng, Xiao-ping; Ge, Wei-wei; Li, He

2011-07-30

419

DelsGate, a robust and rapid gene deletion construction method.  

PubMed

With the increasing availability of fungal genome sequences there is great demand for fast, simple high-throughput methods to generate constructs for gene deletion. Here we describe a method that combines PCR and Gateway cloning technology together with use of the I-SceI homing endonuclease to generate precise deletion constructs in a very simple, universal and robust manner in just 2 days. These constructs are then used to produce deletion mutants in the organism of interest following applicable methods for that species. In establishing this protocol we determined empirically that 1 kb was a suitable flank length to facilitate homologous recombination in our species of interest, Ustilago maydis. The method, which we have named DelsGate (Deletions via Gateway), consists of standard PCR of only the 5' and 3' 1 kb gene flanks directly followed by in vitro Gateway cloning and final generation of the circular deletion construct by in vivo recombination in Escherichia coli. For use in DelsGate we have modified a Gateway cloning vector to include selectable markers for transformation of Ascomycetes and the Basidiomycete fungus U. maydis which causes corn smut disease. We have tested the reproducibility of the DelsGate approach by generating deletion constructs for 12 U. maydis genes. Although not tested here, the PCR and transformation steps of DelsGate should be well suited for high-throughput approaches to gene deletion construction in fungal species. DelsGate has the potential to be universal for all organisms with efficient transformation and homologous recombination systems. PMID:18248826

García-Pedrajas, María D; Nadal, Marina; Kapa, Laura B; Perlin, Michael H; Andrews, David L; Gold, Scott E

2007-11-19

420

Percepcion de los profesores universitarios acerca del concepto cultura cientifica y de sus implicaciones en el nuevo bachillerato del Recinto de Rio Piedras de la Universidad de Puerto Rico  

NASA Astrophysics Data System (ADS)

El Senado Academico del Recinto de Rio Piedras de la Universidad de Puerto Rico aprobo en el ano academico 2005-2006 la Certificacion 46, que contiene los lineamientos de un nuevo bachillerato. Este nuevo bachillerato introdujo cambios significativos en el curriculo tradicional. Entre ellos se encuentra la reduccion del componente de educacion general y el de Ciencias Biologicas en particular. La reduccion de creditos en el componente de Ciencias Biologicas ha obligado a reevaluar el concepto de cultura cientifica que desarrollan esos cursos. El proposito del estudio consistio en auscultar las percepciones de los profesores de las Facultades de Administracion de Empresas, Humanidades, Ciencias Sociales, Ciencias Naturales, Educacion y Estudios Generales del Recinto de Rio Piedras de la Universidad de Puerto Rico en torno al concepto de cultura cientifica, los contenidos disciplinares del curso de Ciencias Biologicas y la reduccion de creditos en el nuevo bachillerato. Las preguntas que guiaron la investigacion fueron: ¿cuales son las percepciones que tienen los profesores de las Facultades de Administracion de Empresas, Ciencias Sociales, Estudios Generales, Ciencias Naturales, Humanidades y Educacion, en torno al concepto de cultura cientifica y los contenidos disciplinares del curso de Ciencias Biologicas? ¿cuales son las percepciones que tienen los profesores de Ciencias Biologicas en torno al concepto cultura cientifica y los contenidos disciplinares del curso de Ciencias Biologicas? ¿existen diferencias significativas por facultad, genero, experiencia, rango y nombramiento en las percepciones que tienen los profesores del Recinto de Rio Piedras de la Universidad de Puerto Rico sobre los elementos que caracterizan la cultura cientifica y los contenidos biologicos que deben tener los egresados del Recinto? ¿que implicaciones curriculares tienen estos testimonios en el desarrollo del concepto de cultura cientifica en el nuevo bachillerato? Para realizar la investigacion se utilizo una metodologia cuantitativa y la herramienta para lograrlo fue una encuesta. La encuesta se analizo estadisticamente mediante analisis de frecuencias y por cientos, prueba ANOVA, prueba t, pruebas Post Hoc de Tukey y de Levene. Tambien se realizo nuevamente una prueba de confiabilidad mediante el alfa de Cronbach. Los resultados reflejaron que los profesores de las Facultades de Administracion de Empresas, Ciencias Sociales, Ciencias Naturales, Educacion, Humanidades, Estudios Generales y el Departamento de Ciencias Biologicas tienen una percepcion muy positiva del concepto de cultura cientifica, los contenidos curriculares de Ciencias Biologicas y del nuevo bachillerato.

Ramos Pastrana, Nilsa

421

Thermal unfolding studies show the disease causing F508del mutation in CFTR thermodynamically destabilizes nucleotide-binding domain 1  

PubMed Central

Misfolding and degradation of CFTR is the cause of disease in patients with the most prevalent CFTR mutation, an in-frame deletion of phenylalanine (F508del), located in the first nucleotide-binding domain of human CFTR (hNBD1). Studies of (F508del)CFTR cellular folding suggest that both intra- and inter-domain folding is impaired. (F508del)CFTR is a temperature-sensitive mutant, that is, lowering growth temperature, improves both export, and plasma membrane residence times. Yet, paradoxically, F508del does not alter the fold of isolated hNBD1 nor did it seem to perturb its unfolding transition in previous isothermal chemical denaturation studies. We therefore studied the in vitro thermal unfolding of matched hNBD1 constructs ±F508del to shed light on the defective folding mechanism and the basis for the thermal instability of (F508del)CFTR. Using primarily differential scanning calorimetry (DSC) and circular dichroism, we show for all hNBD1 pairs studied, that F508del lowers the unfolding transition temperature (Tm) by 6–7°C and that unfolding occurs via a kinetically-controlled, irreversible transition in isolated monomers. A thermal unfolding mechanism is derived from nonlinear least squares fitting of comprehensive DSC data sets. All data are consistent with a simple three-state thermal unfolding mechanism for hNBD1 ± F508del: N(±MgATP) ? IT(±MgATP) ? AT ? (AT)n. The equilibrium unfolding to intermediate, IT, is followed by the rate-determining, irreversible formation of a partially folded, aggregation-prone, monomeric state, AT, for which aggregation to (AT)n and further unfolding occur with no detectable heat change. Fitted parameters indicate that F508del thermodynamically destabilizes the native state, N, and accelerates the formation of AT.

Protasevich, Irina; Yang, Zhengrong; Wang, Chi; Atwell, Shane; Zhao, Xun; Emtage, Spencer; Wetmore, Diana; Hunt, John F; Brouillette, Christie G

2010-01-01

422

CHEK2*1100delC homozygosity in the Netherlands-prevalence and risk of breast and lung cancer.  

PubMed

The 1100delC mutation in the CHEK2 gene has a carrier frequency of up to 1.5% in individuals from North-West Europe. Women heterozygous for 1100delC have an increased breast cancer risk (odds ratio 2.7). To explore the prevalence and clinical consequences of 1100delC homozygosity in the Netherlands, we genotyped a sporadic breast cancer hospital-based cohort, a group of non-BRCA1/2 breast cancer families, and breast tumors from a tumor tissue bank. Three 1100delC homozygous patients were found in the cohort of 1434 sporadic breast cancer patients, suggesting an increased breast cancer risk for 1100delC homozygotes (odds ratio 3.4, 95% confidence interval 0.4-32.6, P=0.3). Another 1100delC homozygote was found in 592 individuals from 108 non-BRCA1/2 breast cancer families, and two more were found after testing 1706 breast tumors and confirming homozygosity on their wild-type DNA. Follow-up data was available for five homozygous patients, and remarkably, three of them had developed contralateral breast cancer. A possible relationship between 1100delC and lung cancer risk was investigated in 457 unrelated lung cancer patients but could not be confirmed. Due to the small number of 1100delC homozygotes identified, the breast cancer risk estimate associated with this genotype had limited accuracy but is probably higher than the risk in heterozygous females. Screening for CHEK2 1100delC could be beneficial in countries with a relatively high allele frequency.European Journal of Human Genetics advance online publication, 8 May 2013; doi:10.1038/ejhg.2013.85. PMID:23652375

Huijts, Petra Ea; Hollestelle, Antoinette; Balliu, Brunilda; Houwing-Duistermaat, Jeanine J; Meijers, Caro M; Blom, Jannet C; Ozturk, Bahar; Krol-Warmerdam, Elly Mm; Wijnen, Juul; Berns, Els Mjj; Martens, John Wm; Seynaeve, Caroline; Kiemeney, Lambertus A; van der Heijden, Henricus F; Tollenaar, Rob Aem; Devilee, Peter; van Asperen, Christi J

2013-05-08

423

Genetic counseling for susceptibility loci and neurodevelopmental disorders: The del15q11.2 as an example.  

PubMed

In recent years, several recurrent copy number variations (CNVs) that confer risk of neurodevelopmental disorders have been identified (e.g., del and dup 16p11.2, del15q13.3, del and dup 1q21.1, del16p13.3, del15q11.2). They are often inherited from an unaffected parent and lack phenotypic specificity. Although there is growing evidence from association studies to consider them as susceptibility CNVs, their clinical utility is debated. Yet the clinician is frequently challenged to deal with these counseling situations without guidelines or consensus. In this report, counseling issues and research opportunities are discussed, with the recurrent 15q11.2 BP1-BP2 (including CYFIP1, NIPA1, NIPA2, TUBGCP5) as an example. Several clinical reports have been published describing patients with del15q11.2 featuring intellectual disability, developmental delay, neurological problems, autism spectrum disorder (ASD), attention problems, speech delay, and dysmorphism. The del15q11.2 was found to be significantly associated with intellectual disability, schizophrenia, epilepsy, and ASD. In this report we discuss how patient-specific and family-specific information may alter the interpretation of del15q11.2 as a contributing factor to the disorder in practical counseling situations. In addition, an association study for ASD in a Belgian Flemish cohort and an overview of reported association studies, clinical reports and genomics data for del15q11.2 are presented. © 2013 Wiley Periodicals, Inc. PMID:24123946

De Wolf, Veerle; Brison, Nathalie; Devriendt, Koenraad; Peeters, Hilde

2013-10-10

424

Development of InDel markers for Brassica rapa based on whole-genome re-sequencing.  

PubMed

Genome-wide detection of short insertion/deletion length polymorphisms (InDels, <5 bp) in Brassica rapa (named the A genome) was performed by comparing whole-genome re-sequencing data from two B. rapa accessions, L144 and Z16, to the reference genome sequence of Chiifu-401-42. In total, we identified 108,558 InDel polymorphisms between Chiifu-401-42 and L144, 26,795 InDels between Z16 and Chiifu-401-42, and 26,693 InDels between L144 and Z16. From these, 639 InDel polymorphisms of 3-5 bp in length between L144 and Z16 were selected for experimental validation; 491 (77%) yielded single PCR fragments and showed polymorphisms, 7 (1%) did not amplify a product, and 141 (22%) showed no polymorphism. For further validation of these intra-specific InDel polymorphisms, 503 candidates, randomly selected from the 639 InDels, were screened across seven accessions representing different B. rapa cultivar groups. Of these assayed markers, 387 (77%) were polymorphic, 111 (22%) were not polymorphic and 5 (1%) did not amplify a PCR product. Furthermore, we randomly selected 518 InDel markers to validate their polymorphism in B. napus (the AC genome) and B. juncea (the AB genome), of which more than 90% amplified a PCR product; 132 (25%) showed polymorphism between the two B. napus accessions and 41 (8%) between the two B. juncea accessions. This set of novel PCR-based InDel markers will be a valuable resource for genetic studies and breeding programs in B. rapa. PMID:22972202

Liu, Bo; Wang, Yan; Zhai, Wen; Deng, Jie; Wang, Hui; Cui, Yang; Cheng, Feng; Wang, Xiaowu; Wu, Jian

2012-09-13

425

Maintaining the competitiveness of the American fisheries society journals: An assessment based on influence and cost-effectiveness [Mantenimiento de la competitividad de las revistas de la Sociedad Americana de Pesquer??as: Una evaluaci??n basada en la influencia y efectividad de costos  

USGS Publications Warehouse

Recent changes in the landscape of scientific publishing prompted the Publications Overview Committee of the American Fisheries Society (AFS) to review the Society's portfolio of scientific journals. We evaluated journals based on metrics in two categories: (1) citation-based measures of the influence of a journal on the scientific literature, and (2) measures of the cost-effectiveness of a journal (citation rate adjusted for subscription cost). Over the long-term, we found that ecology journals had far stronger citation-based influence than fisheries and aquatic sciences journals, and that journals publishing primarily basic research had stronger influence than journals publishing applied research (including four AFS journals and Fisheries magazine). In evaluating the current status of fisheries and aquatic sciences journals, we found that metrics of influence and cost-effectiveness provided considerably different portrayals of journals relative to their peers. In terms of citation-based influence, we found that the AFS journal Transactions of the American Fisheries Society (TAFS) and Fisheries magazine were competitive with highly regarded peer fisheries journals, but that North American Journal of Aquaculture (NAJA) and Journal of Aquatic Animal Health (JAAH) were less influential than their peers. The citation-based influence of North American Journal of Fisheries Management (NAJFM) was intermediate between TAFS/Fisheries and NAJA/JAAH. For journals like NAJFM and NAJA, we expect that much of the scientific influence on policy and management is not captured by citations in the primary literature, and alternative methods of evaluation may be needed. All of the AFS journals ranked highly with regard to cost-effectiveness because their subscription costs are low, and these rankings are in accordance with membership needs and the strategic mission of AFS to provide broad and timely dissemination of scientific information. We conclude by suggesting ways to increase the influence of AFS journals without compromising their accessibility and affordability, and offer advice about methods and frequency for future journal evaluations. ?? 2009.

Hewitt, D. A.; Link, J. S.; Wahl, D. H.; Cooke, S. J.; Mather, M. E.

2009-01-01

426

Epidemiological surveillance of ovine hydatidosis in Tierra del Fuego, Patagonia Argentina, 1997-1999.  

PubMed

Cystic echinococcosis is the most prevalent zoonosis in Tierra del Fuego province, Argentina, with important economic, productive and public health consequences. The present work was performed to determine the ovine prevalence in Tierra del Fuego, Argentina, as well as to evaluate the quality of diagnostic systems in slaughterhouses. Moreover, genetic analyses to characterize the strain of Echinococcus granulosus involved in the region were done. The first actions to perform a diagnosis of the epidemiological situation of hydatidosis in Tierra del Fuego were done between 1976 and 1977. A canine prevalence of 80% and an ovine prevalence of 55% results were obtained. Since 1979 the control program of Hydatidosis of Tierra del Fuego was implemented. It was based on semiannual canine anthelmintic treatment with praziquantel at dose of 5mg/kg, and complemented with sanitary education and canine and ovine epidemiological surveillance. During May 1997-January 1999: 5,916 sheep coming from 20 farms of the programmatic area were evaluated. In the lamb category, hydatid cysts were not found. In the adults category, 62 infected animals were found (3.2%). The ovine prevalence was 1.1% and there was 100% of coincidence between diagnosis in the slaughterhouse, re-inspection in the laboratory and histopathological study. The marked decrease in the prevalence observed for sheep infection evidenced a destabilization of the biological cycle of the parasite. This could be explained by the application of a control program with uninterrupted systematic actions. Polymerase chain reaction-ribosomal ITS-1 DNA (rDNA) restriction fragment length polymorphism (PCR-RFLP) analysis and partial sequencing of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene were used to characterize E. granulosus isolates collected from different regions of Tierra del Fuego to determine which genotypes occurred in this region. The results revealed the presence of the G1 genotype (sheep-dog strain). This is the first time that a molecular analysis was performed for the E. granulosus isolates from Tierra del Fuego. PMID:16504405

Zanini, Fabián; Gonzalo, Roberto; Pérez, Héctor; Aparici, Inés; Soto, Ximena; Guerrero, Juvenal; Cerrone, Gloria; Elissondo, Celina

2006-02-28

427

ARTE RUPESTRE EN ASENTAMIENTOS DEL PERÍODO TARDÍO EN LOS VALLES DE LLUTA Y AZAPA, NORTE DE CHILE ROCK ART IN LATE HORIZON SETTLEMENTS IN THE VALLEYS OF LLUTA AND AZAPA, NORTHERN CHILE  

Microsoft Academic Search

Este trabajo busca contribuir a la discusión sobre el proceso de expansión del estado Inka hacia las áreas periféricas del sur de los Andes, particularmente en los valles costeros Lluta y Azapa del extremo norte de Chile. Este proceso es analizado explorando el rol del arte rupestre -como soporte material de transmisión de ideología- en el proceso de integración que

Daniela Valenzuela; Calogero M. Santoro; Álvaro Romero

2004-01-01

428

The 'Del'fin' high-power laser facility for heating spherical thermonuclear targets  

NASA Astrophysics Data System (ADS)

A review is presented of the 12-channel Del'fin laser fusion system for the high-temperature heating of thermonuclear targets in spherical irradiation geometry. Del'fin consists of a neodymium laser (with limit output energy of 10 kJ, pulse duration of 0.1-1.0 nsec, and radiation divergence of 0.0005 rad), a vacuum chamber for the laser-plasma interaction, and diagnostic probes for laser and plasma. Particular consideration is given to the focusing system which makes it possible to achieve a high degree of symmetry of spherical irradiation for maximum flux densities at the target surface (10 to the 15th W/sq cm). The problem of maximizing spherical target heating in the laser fusion system is considered.

Basov, N. G.; Bykovskii, N. E.; Danilov, A. E.; Kalashnikov, M. P.; Krokhin, O. N.; Kruglov, B. V.; Mikhailov, Iu. A.; Osetrov, V. P.; Pletnev, N. V.; Rode, A. V.

1980-03-01

429

History and use of del Nido cardioplegia solution at Boston Children's Hospital.  

PubMed

Cardioplegia is an integral and essential method of myocardial protection for patients of all ages requiring cardiac surgery in which the heart must be stopped. Numerous cardioplegia solutions and delivery methods have been developed. The del Nido cardioplegia solution has been in use for 18 years at Boston Children's Hospital. This is a unique four parts crystalloid to one part whole blood formulation that is generally used in a single-dose fashion. Although the formulation was originally developed for use in pediatric and infant patients, its use for adult cardiac surgery has been expanding. National and international inquiries to our institution regarding this cardioplegia have been increasing over the last 2 years. We present the developmental history, supporting theory, and current protocol for use of what is now referred to as del Nido cardioplegia. PMID:23198389

Matte, Gregory S; del Nido, Pedro J

2012-09-01

430

Detection of super-soft emission in nova V339 Del  

NASA Astrophysics Data System (ADS)

Swift has been regularly observing the fourth LAT-detected classical nova (ATEL #5302) V339 Del (Nova Del 2013; PNV J20233073+2046041) since its outburst on 2013 August 14. Once optical loading had been correctly accounted for (ATel #5429), a real X-ray source was first detected on September 19. The XRT 0.3-10 keV X-ray count rate has been slowly rising since this time, from a value of (1.8 +0.7/-0.6)x10-3 count s-1 on September 19 to (7 +/- 2)x10-3 count s-1 on October 8 [these count rates are from grade 0 (single pixel) events only, to mitigate any residual optical loading effects].

Page, K. L.; Osborne, J. P.; Kuin, N. P. M.; Woodward, C. E.; Schwarz, G. J.; Starrfield, S.; Shore, S. N.; Walter, F. M.

2013-10-01

431

Seismic stratigraphic analysis of the Punta del Este basin, offshore Uruguay, South America  

SciTech Connect

The Punta del Este part of the greater Salado basin is relatively unexplored with only two wells having been drilled, both in the updip part of the basin. These two wells have contributed some understanding of the stratigraphic succession but have failed to adequately assess the basin's true hydrocarbon potential. This paper constitutes one of the first detailed, comprehensive published accounts of this basin and is a prerequisite for future exploration in the area. In this paper, the authors give the results and major conclusions of a regional seismic stratigraphic analysis of the Punta del Este basin, offshore Uruguay. This paper is one of the first detailed, comprehensive published accounts of the tectonic and sedimentary evolution of this basin. The study area covers approximately 15,000 km{sup 2} (5,790 mi{sup 2}) of the continental shelf, from the shoreline to the 200-m (655-ft) shelf isobath.

Stoakes, F.A.; Campbell, C.V. (Stoakes Campbell Geoconsulting Ltd., Calgary, Alberta (Canada)); Cass, R. (Arvec Consulting Ltd., Calgary, Alberta (Canada)); Ucha, N. (ANCAP, Montivideo (Uruguay))

1991-02-01

432

From badge of pride to cause of stigma: combatting mal del pinto in Mexico.  

PubMed

Mal del pinto is a dermatological disease characterized by discoloured patches of skin on the face and body. It has been present in what is now the territory of Mexico from before the Spanish conquest up to recent times. Though early concerns for mal del pinto as a public health problem can be traced back to the late 19th century, no campaign to combat the disease was undertaken until the second half of the 20th. Thanks to the effectiveness of treatment with penicillin, the fight against this illness--which was once assumed as a symbol of pride--enjoyed a broader acceptance among the population that other health campaigns. PMID:23332859

Carrillo, Ana María

2013-01-17

433

Down and Turner syndromes in a female infant with 47,X,del(X)(p11),+21  

Microsoft Academic Search

This report describes a female infant with a 47,X,del(X)(p11),+21 karyotype who has clinical features of both Down and Turner syndromes. The majority of her clinical features are suggestive of Down syndrome.

John T. Martsolf; M. Ray; Fred Bauder; Rod Boychuk; J. D. Armstrong

1977-01-01

434

75 FR 39668 - Notice of Availability of Final Environmental Impact Statement for the Proposed Rio del Oro...  

Federal Register 2010, 2011, 2012, 2013

...Rio del Oro Specific Plan Project, in Sacramento County, CA, Corps Permit Application...Army Corps of Engineers (USACE), Sacramento District has prepared a Final Environmental...use development within south-eastern Sacramento County. On December 8, 2006,...

2010-07-12

435

Phase II, Final Project Report, Paso del Norte Watershed Council, Coordinated Water Resources Database, and GIS Project.  

National Technical Information Service (NTIS)

The Coordinated Water Resources Database and GIS Project was developed to provide improved access to regional water resources data in the Paso del Norte region for regional water stakeholders to make timely decisions in water operations and flood control....

C. Brown M. Bourdon Z. Sheng

2007-01-01

436

A history of 'uel': From Latin to Castilian \\/ Una història de 'uel': Del llatí al castellà  

Microsoft Academic Search

Centrant-nos específicament en les evidències procedents de la Península Ibèrica,\\u000aen aquest article s'hi traça la història de la partícula disjuntiva llatina 'uel';, del\\u000aseu ús en llatí clàssic a la seva eventual desaparició en romanç. S'hi estudien\\u000ales semblances entre el llatí i el romanç, i s'hi prova que tot i que encara es pot\\u000aaprendre molt concentrant-nos només

Neil Allies

2008-01-01

437

La externalizacion del trabajo agrario y las empresas de servicios a la agricultura  

Microsoft Academic Search

Resumen: En este art�culo se analiza el proceso de externalizaci�n de las labores agrarias y el consiguiente desarrollo de una agricultura terciarizada. La fragmentada dimensi�n laboral y econ�mica de las explotaciones agrarias determina el proceso de externalizaci�n de las tareas agrarias en la medida en que no permite ni la incorporaci�n de innovaciones que exige el desarrollo del sistema alimentario

Alicia Langreo Navarro; Sociedad de Estudios

2002-01-01

438

Patrones alimenticios y valoración del estado nutricional en población adulta atendida en la Atención Primaria  

Microsoft Academic Search

INTRODUCCIÓN: Los patrones alimenticios influyen en la salud del momento y pueden determinarla a largo plazo.\\u000aOBJETIVOS: Identificar los hábitos alimenticios y el estado nutricional de la población entre 18 y 44 años de edad atendida en un centro de atención primaria y estudiar los factores asociados.\\u000aTIPO DE ESTUDIO: Transversal.\\u000aEMPLAZAMIENTO: Área básica urbana.\\u000aSUJETOS: Muestra aleatoria de 201

Matilde González Solanellas; Marta Puig Soler; Ana Romagosa Perez-Portabella; Clàudia Caselles Montagut; Montserrat Grau Carod; Hegoi Segurola Gurrutxaga; Santiago Lancho Lancho; Edurne Zabaleta del Olmo

2009-01-01

439

Pasado, presente y futuro de las dimensiones pública y social en el desarrollo conceptual del marketing  

Microsoft Academic Search

Resumen  Bien sabido es lo conveniente que resulta delimitar conceptual y metodológicamente un fenómeno o circunstancia antes de proceder\\u000a a su estudio, afirmación que cobra pleno sentido si a lo que se está haciendo referencia es a las dimensiones páblica y social\\u000a del marketing. A partir de dicho planteamiento, en el presente trabajo se lleva a cabo un análisis de la

José Luis Vézquez Burguete

2004-01-01

440

The cyclic hierarchy of the 'Purbeckian' Sierra del Pozo Section, Lower Cretaceous (Berriasian), southern Spain  

Microsoft Academic Search

The lowermost Cretaceous (Berriasian) Sierra del Pozo Formation is divisible into metre-scale cycles that are bundled in a four-tiered hierarchy of cycles and cyclic sequences. At the smallest scale, sixth-order cycles, thought to be the product of precessionally forced sea-level fluctuations (c. 20 ka), average less than a metre in thickness, shallow upward and are bounded by surfaces where deeper

EDWIN J. A NDERSON

2004-01-01

441

Optimización mediante algoritmos genéticos de la gestión del agua en el regadío  

Microsoft Academic Search

El uso de un recurso como el agua, esencial y escaso, tiene gran trascendencia ambiental, social, económica,\\u000apolítica, etc., siendo su adecuada gestión fundamental para conseguir la sostenibilidad de sus aprovechamientos.\\u000aPara alcanzar ese objetivo, la agricultura actual necesita modelos de ayuda a la toma de decisiones para\\u000ala gestión y explotación agrícola.\\u000aSe presenta un modelo de optimización del

Pedro Carrión; Eulogio López; José Fernando Ortega; Arturo de Juan

1970-01-01

442

Challenges of modeling current very large lahars at Nevado del Huila Volcano, Colombia  

Microsoft Academic Search

Nevado del Huila, a glacier-covered volcano in the South of Colombia’s Cordillera Central, had not experienced any historical\\u000a eruptions before 2007. In 2007 and 2008, the volcano erupted with phreatic and phreatomagmatic events which produced lahars\\u000a with flow volumes of up to about 300 million m3 causing severe damage to infrastructure and loss of lives. The magnitude of these lahars

Raphael Worni; Christian Huggel; M. Stoffel; B. Pulgarín

443

Gleevec disminuye la recidiva del cáncer en pacientes con tumor estromal gastrointestinal primario  

Cancer.gov

Los resultados preliminares de un estudio clínico grande aleatorio y controlado con placebo, en pacientes con tumor estromal gastrointestinal (GIST) primario, un tipo de tumor que por lo general se encuentra en el estómago o en el intestino delgado, demostraron que los pacientes a los que se administró mesilato de imatinib (Gleevec®) luego de la extirpación total de su tumor tuvieron una probabilidad significativamente menor de padecer una recidiva del cáncer en comparación con aquellos a los que no se administró imatinib.

444

The CASP8 -652 6N del promoter polymorphism and breast cancer risk: a multicenter study  

Microsoft Academic Search

A recent study on an Asian population reported a six-nucleotide insertion-deletion polymorphism (-652 6N del) in the CASP8 promoter region to be strongly associated with a decreased risk of multiple types of cancer, including breast cancer (BC).\\u000a Here, we investigate the effect of this deletion in four independent large European BC case-control studies, including data\\u000a from a total of 7,753

Bernd Frank; Sushila H. Rigas; Justo Lorenzo Bermejo; Miriam Wiestler; Kerstin Wagner; Kari Hemminki; Malcolm W. Reed; Christian Sutter; Barbara Wappenschmidt; Sabapathy P. Balasubramanian; Alfons Meindl; Marion Kiechle; Peter Bugert; Rita K. Schmutzler; Claus R. Bartram; Christina Justenhoven; Yon-Dschun Ko; Thomas Brüning; Hiltrud Brauch; Ute Hamann; Paul P. D. Pharoah; Alison M. Dunning; Karen A. Pooley; Douglas F. Easton; Angela Cox; Barbara Burwinkel

2008-01-01

445

The distribution and regeneration of Boswellia papyrifera (Del.) Hochst. in Eritrea  

Microsoft Academic Search

Boswellia papyrifera (Del.) Hochst. is a deciduous gum-producing multipurpose perennial tree species growing in Sudanian and Sahelian regions. The tree is tapped on the stem for oleo-gum called olibanum (true frankincense). Land clearing for agriculture and un-regulated grazing are threatening the future of the natural Boswellia woodlands in Eritrea. Against this background, a study was carried out to investigate the

Ogbazghi Woldeselassie

2001-01-01

446

Physical, morphological and chemical characteristics, oil recovery and fatty acid composition of Balanites aegyptiaca Del. kernels  

Microsoft Academic Search

Balanites aegyptiaca Del. kernels were chemically,physically and morphologically characterized. Crude oil (49.0%) andcrude protein (32.4%) were the two major constituents of the kernels.Phytic acid content was relatively high compared to other legumes. Incontrast, antitryptic activities of the kernel flours were very low.Sapogenin contents of the full fat, defatted and testa flours were 1.5, 2.7and 3.0%, respectively. The hardness of the

A. M. Mohamed; W. Wolf; W. E. L. Spieß

2002-01-01

447

GIS based Relative Tsunami Hazard Maps for Northern California, Humboldt and Del Norte Counties  

Microsoft Academic Search

Tsunami hazard maps are generated using a geographical information systems (GIS) approach to depict the relative tsunami hazard of coastal Humboldt and Del Norte Counties in northern California. Maps are composed for the Humboldt Bay, Eel River, and Crescent City regions and available online at http:\\/\\/www.humboldt.edu\\/~geodept\\/earthquakes\\/rctwg\\/toc.html . In contrast to previous mapping efforts that utilize a single line to represent

J. R. Patton; L. A. Dengler

2004-01-01

448

CHEK2 1100delC and polygenic susceptibility to breast cancer and colorectal cancer  

Microsoft Academic Search

Approximately 15-25% of breast cancers are identified in women with a family history of breast cancer. Yet, germline mutations in the currently known breast cancer susceptibility genes account for only one-third of familial breast cancer cases. In 2002, our research group had identified the CHEK2 1100delC mutation as a breast cancer susceptibility allele. It was estimated that this mutation confers

M. Wasielewski

2009-01-01

449

Antimicrobial activity of flavonoids in medicinal plants from Tafí del Valle (Tucumán, Argentina).  

PubMed

Preliminary studies of flavonoids have been realised in five native species from Tafí del Valle (Tucumán, Argentina) used in popular medicine. Most of compounds detected were flavonoids mono and dihydroxylated in B ring. Screening for antimicrobial activity against Gram positive and Gram negative microorganisms has been realised with Lippia turbinata, Satureja parvifolia, Sambucus peruviana, Verbena officinalis and Chenopodium graveolens. The total extracts of flavonoids of each plant were tested and four species studied showed antimicrobial activity. PMID:11025172

Hernández, N E; Tereschuk, M L; Abdala, L R

2000-11-01

450

Urban agriculture, poverty alleviation, and gender in Villa María del Triunfo, Peru  

Microsoft Academic Search

This chapter describes gender mainstreaming in the multi-stakeholder action- planning process (MPAP) in the district of Villa María del Triunfo, which began in 2005. It refl ects the inclusion of a gender perspective in every stage of the process developed, and the project's contribution to promoting gender equity in urban agriculture. The multi-stakeholder action-planning process was conceived in the city

Noemí Soto; Gunther Merzthal; Maribel Ordoñez; Milagros Touzet

451

Growth patterns of Nothofagus pumilio trees along altitudinal gradients in Tierra del Fuego, Argentina  

Microsoft Academic Search

The influence of ecological factors resulting from altitude on growth characteristics of Nothofagus pumilio was analyzed. N. pumilio forests grow in the southern Andes from near sea level to 600 m.a.s.l. Eight study sites were selected in the southwestern\\u000a area of Tierra del Fuego, on north- and south-facing slopes, and distributed along an altitudinal gradient (200–600 m.a.s.l.).\\u000a Growth cycles throughout

Guillermina Massaccesi; Fidel A. Roig; Guillermo J. Martínez Pastur; Marcelo D. Barrera

2008-01-01

452

Mössbauer study of archaeological ceramics from Valle del Alto Sinu (Colombia)  

NASA Astrophysics Data System (ADS)

A physico-chemical characterization of ceramic samples from the arachaeological sites of El Cabrero, El Gallo, and El Frasquillo (Valle del Alto Sinú) is presented. Extensive use of Mössbauer spectroscopy data reveals that the currently used typological classification scheme of Dolmatoff is related to the production technology of the different artifacts. In addition, a model for firing conditions for “Rojo Sencillo”, “Tierra Impresa”, “Tierra Incisa”, and “Blanco” types of ceramics is proposed.

Useche, L. A.; Gaviria, S.; Alabart, J. R.; Fontcuberta, J.

1990-07-01

453

Lack of founding Amerindian mitochondrial DNA lineages in extinct aborigines from Tierra del Fuego-Patagonia  

Microsoft Academic Search

Ancient DNA from bones and teeth of 60 individuals from four extinct human populations from Tierra del Fuego-Patagonia (Selknam, Yamana, Kaweskar and Aonikenk) has been extracted and the mitochondrial DNA (mtDNA) amplified by using the polymerase chain reaction. High-resolution analysis of endonuclease restriction site variation in the mtDNA and sequencing of its hypervariable non-coding control region, revealed complete absence of

Carles Lalueza; Alejandro Pérez-Pérez; Eva Prats; Lluís Cornudella; Daniel Turbón

1997-01-01

454

Groundwater flow modelling of the regional aquifer of the Pampa del Tamarugal, northern Chile  

Microsoft Academic Search

The Pampa del Tamarugal Aquifer (PTA) is an important source of groundwater in northern Chile. In this study, a groundwater\\u000a flow model of this aquifer is developed and calibrated for the period 1983–2004. The model reproduces the observed flow-field\\u000a and the water balance components reasonably well. Five scenarios are defined to evaluate the response to different pumping\\u000a situations. These scenarios

Rodrigo Rojas; Alain Dassargues

2007-01-01

455

A sole del(15q) anomaly in post-myelodysplasia acute myeloid leukemia  

Microsoft Academic Search

We report the second case of post-myelodysplasia acute myeloid leukemia (post-MDS AML) with a sole chromosome change del(15q). This anomaly is rarely seen. To our knowledge, only seven cases so far have been reported in human neoplasias, including one case each of acute myeloid leukemia (AML), acute lymphoid leukemia, post myelodysplasia AML, myelodysplastic syndrome, myelofibrosis, macroglobulinemia, Hodgkin's lymphoma and uterine

Naoyuki Yahata; Kazuma Ohyashiki; Osamu Iwase; Yukihiko Kimura; Atsushi Kodama; Katsuyski Fukutake; Keisuke Toyama

1998-01-01

456

Metabolismo di cellule adipose umane isolate in vitro. I. Effetto del glucosio e dell'Insulina  

Microsoft Academic Search

Riassunto L'insulina stimola l'incorporazione dell' 114C del glucosio nel CO2 prodotto da cellule adipose umane isolatein vitro. Tale effetto sembra essere evidente solo con concentrazioni abbastanza elevate dell'ormone, nelle condizioni sperimentali descritte nel presente lavoro. Analogamente, anche l'effetto antilipolitico dell'insulina è evidenziabile, in modesta entità, solo con concentrazioni relativamente elevate dell'ormone nelmedium di incubazione. La presenza di glucosio nelmedium di

Guido Pozza; Alberto Ghidoni; Edda Sanesi; Augusta Pappalettera; Angela Tognetti

1970-01-01

457

Studio degli anticorpi anti-insulina del siero umano in soggetti normali e diabetici  

Microsoft Academic Search

Riassunto Vengono presentati i risultati ottenuti con l'impiego di una metodica di semplice esecuzione per la ricerca di anticorpi anti-insulina, basata sull'impiego di insulina I125 o I131 e sulla successiva precipitazione con alcool assoluto del complesso antigene-anticorpo. Gli anticorpi anti-insulina sono stati riscontrati con grande frequenza solo in pazienti diabetici già sottoposti a trattamento insulinico. Un elevato titolo di anticorpi

Alberto Ghidoni; Giuseppe Sorgato; Edda Sanesi; Guido Pozza

1968-01-01

458

Paleomagnetism and 40Ar\\/39Ar Chronology of Lavas from Meseta del Lago Buenos Aires, Patagonia  

Microsoft Academic Search

As part of a larger project investigating paleosecular variation in South America, 36 paleomagnetic and geochronology sites were sampled on Meseta del Lago Buenos Aires (47°S, 289°E), 200 km east of the active plate boundary in southern Patagonia. Basaltic lavas sampled range from late Miocene to late Pleistocene yet most of the individual lavas are younger than 3.3 Ma. Thirty-eight

Laurie L. Brown; Brad S. Singer; Matthew L. Gorring

2004-01-01

459

Ricerche sull’azione del campo magnetico terrestre sopra i corpuscoli della radiazione penetrante  

Microsoft Academic Search

\\u000a Sunto  Se la radiazione penetrante giunge al limite dell’atmosfera come una radiazione corpuscolare avente un’energia di alcuni miliardi\\u000a di volt-elettrone, il campo magnetico terrestre dovrebbe dar luogo ad una forte dissimetria nella sua distribuzione di intensità\\u000a rispetto al piano del meridiano magnetico. L’esperiema eseguita per mettere in evidenza questa dissimetria ha dato esito negativo.

Bruno Rossi

1931-01-01

460

Chemostratigraphy of the Lower Arroyo del Soldado Group (Vendian, Uruguay) and Palaeoclimatic Implications  

Microsoft Academic Search

New C- and O-isotopic determinations from the Vendian lower Arroyo del Soldado Group are reported and combined with sedimentologic and biostratigraphic data. On the basis of different geochemical and petrographic criteria, the primary nature of the C- and Sr-isotopic signature is shown. Positive d13C-values characterize the mainly siliciclastic upper Yerbal Formation, which contains oxide-facies BIF and a diverse assemblage of

Claudio Gaucher; Alcides Nóbrega Sial; Gonzalo Blanco; Peter Sprechmann

2004-01-01

461

The Icelandic founder mutation BRCA2 999del5: analysis of expression  

Microsoft Academic Search

INTRODUCTION: A founder mutation in the BRCA2 gene (BRCA2 999del5) accounts for 7–8% of female breast cancers and for 40% of male breast cancers in Iceland. If expressed, the mutant gene would encode a protein consisting of the first 256 amino acids of the BRCA2 protein. The purpose of this study was to determine whether this mutant protein is produced

Evgenia K Mikaelsdottir; Sigridur Valgeirsdottir; Jorunn E Eyfjord; Thorunn Rafnar

2004-01-01

462

Sul problema del cambiamento di variabili nella teoria delle distribuzioni (II)  

Microsoft Academic Search

Riassunto  Si espongono nuove considerazioni relative al problema del cambiamento di variabili nella teoria delle distribuzioni, in aggiunta\\u000a a quelle svolte in un nostro precedente lavoro. Nel primo numero si tratterà il problema della derivazione delle distribuzioni\\u000a trasformate in analogia al teorema classico sulla derivazione delle funzioni composte. Nei numeri successivi si tratterà soprattutto\\u000a della estensione, al caso di più variabili,

S. Albertoni; M. Cugiani

1953-01-01

463

SPInDel: a multifunctional workbench for species identification using insertion/deletion variants.  

PubMed

The majority of the available methods for the molecular identification of species use pairwise sequence divergences between the query and reference sequences (DNA barcoding). The presence of multiple insertions and deletions (indels) in the target genomic regions is generally regarded as a problem, as it introduces ambiguities in sequence alignments. However, we have recently shown that a high level of species discrimination is attainable in all taxa of life simply by considering the length of hypervariable regions defined by indel variants. Each species is tagged with a numeric profile of fragment lengths-a true numeric barcode. In this study, we describe a multifunctional computational workbench (named SPInDel for SPecies Identification by Insertions/Deletions) to assist researchers using variable-length DNA sequences, and we demonstrate its applicability in molecular ecology. The SPInDel workbench provides a step-by-step environment for the alignment of target sequences, selection of informative hypervariable regions, design of PCR primers and the statistical validation of the species-identification process. In our test data sets, we were able to discriminate all species from two genera of frogs (Ansonia and Leptobrachium) inhabiting lowland rainforests and mountain regions of South-East Asia and species from the most common genus of coral reef fishes (Apogon). Our method can complement conventional DNA barcoding systems when indels are common (e.g. in rRNA genes) without the required step of DNA sequencing. The executable files, source code, documentation and test data sets are freely available at http://www.portugene.com/SPInDel/SPInDel_webworkbench.html. PMID:22978681

Carneiro, João; Pereira, Filipe; Amorim, António

2012-09-15

464

Dysmorphic Villi Mimicking Partial Mole in a Case with del(18)(q21)  

Microsoft Academic Search

In this article, we report the histopathologic findings in a placenta from an early second trimester abortion. The placental villi showed prominent scalloping with many intravillous trophoblastic pseudoinclusions and mild trophoblastic hyperplasia, mimicking the morphology of partial hydatidiform mole. The placental karyotype was 46,XY,del(18)(q21). These histopathologic changes have been previously described in numerical chromosomal aberrations like triploidy, tetraploidy, and trisomies,

Dinesh Rakheja; Kathleen S. Wilson; Beverly B. Rogers

2004-01-01

465

Germline BRCA1 185delAG mutations in Jewish women with breast cancer  

Microsoft Academic Search

SummaryBackground We aimed to find out the proportion of breast cancers in Ashkenazi Jewish women attributable to the frameshift mutation at position 185 involving the deletion of adenine and guanine (185delAG) in the breast cancer gene BRCA1.Methods We studied 107 Ashkenazi Jewish women with breast cancer seen at medical oncology and genetic counselling clinics in New York over a three

K Offit; P McGuire; A Schluger; H Hampel; K Brown; T Gilewski; L Norton; J Swensen; S Neuhausen; M Skolnick; D Goldgar

1996-01-01

466

El nou cremallera de Montserrat (1): significarà la recuperació del ferrocarril més popular de Catalunya  

Microsoft Academic Search

L'article recull, en aquesta primera part, els antecedents històrics del cremallera de Montserrat fons al replantejament de la necessitat de reinstaurar aquest transport públic després de la seva clausura el 1957. S'hi presenten les alternatives que es van estudiar prèviament a l'adopció d'un nou sistema de cremallera que permetrà alhora serveis directes amb unes altres poblacions de Catalunya.

Carles Salmeron i Bosch

1990-01-01

467

Atmospheric background trace elements deposition in Tierra del Fuego region (Patagonia, Argentina), using transplanted Usnea barbata lichens  

Microsoft Academic Search

Lichen, Usnea barbata, transplants taken from Tierra del Fuego (south Patagonia, Argentina) were tested as potential biomonitors of atmospheric\\u000a airborne deposition in an apparently pristine environment. In 2005, lichens were sampled in a reference site (n?=?31) and transplanted in the northern Region of Tierra del Fuego. After, respectively, 1 month and 1 year of exposure, we\\u000a collected them. The aim of the

Marcelo Enrique Conti; Maria Grazia Finoia; Beatrice Bocca; Giustino Mele; Alessandro Alimonti; Anna Pino

468

DEL RBC transfusion should be avoided in particular blood recipient in East Asia due to allosensitization and ineffectiveness*  

PubMed Central

Previously, both primary and secondary anti-D alloimmunizations induced by “Asian type” DEL (RHD1227A allele) were observed in two incidents. We investigated how often these alloimmunization events occur. The transfusions of any D-negative patients were investigated in the First Affiliated Hospital of Xi’an Jiaotong University Medical College, China, during the entire 2009. The antigens of D, C, c, E, and e were routinely serotyped. The “Asian type” DEL variant was genotyped and the RHD heterozygote was determined through two published methods. The changes in anti-D levels were monitored by the indirect antiglobulin test (IAT) and flow cytometry. Thirty D-negative transfused patients were included in the study. We focused on 11 recipients who were transfused with packed red blood cells (RBCs) from DEL donors at least one time. Of those 11 recipients, seven were anti-D negative before transfusion and four were anti-D positive (one patient with an autoantibody). One of the seven pre-transfusion anti-D negative patients produced a primary-response anti-D after being transfused with 400 ml of DEL blood twice. All four pre-transfusion antibody positive patients were not observed hemoglobin (Hb) levels increased, as expected after transfusions. Two patients had an increase in anti-D from 1:8 to 1:64 by IAT, which was also shown by flow cytometry. None of the patients experienced an acute hemolytic episode. Our data indicated that the primary anti-D induced by DEL transfusion or the secondary anti-D elevated by DEL in a truly D-negative patient might not be unusual. We suggest that a truly D-negative childbearing-aged woman should avoid DEL transfusion to protect her from primary anti-D allosensitization. In addition, anti-D positive recipients should also avoid DEL red cell transfusion due to the delayed hemolytic transfusion reaction (DHTR).

Shao, Chao-peng; Wang, Bao-yan; Ye, Shi-hui; Zhang, Wen-li; Xu, Hua; Zhuang, Nai-bao; Wu, Xiao-ying; Xu, Heng-gui

2012-01-01

469

Frequency of the CHEK2 1100delC Mutation among Women with Breast Cancer: An International Study  

Microsoft Academic Search

A founder allele in the CHEK2 gene (1100delC) has been associated with an elevated risk of breast cancer. This allele is responsible for the majority of CHEK2-associated breast cancers in women from northern European countries; however, within Europe, it seems to be rare in countries that are close to the Mediterranean. The frequency of the 1100delC allele has not been

Shiyu Zhang; Catherine M. Phelan; Phil Zhang; Francois Rousseau; Parviz Ghadirian; William Foulkes; Nancy Hamel; David McCready; Maureen Trudeau; Henry Lynch; Douglas Horsman; Zeba Aziz; Magda Gomes; Mauricio Magalhaes Costa; Alexander Liede; Aletta Poll; Ping Sun; Steven A. Narod

470

Frequency of CHEK2*1100delC in New York breast cancer cases and controls  

Microsoft Academic Search

BACKGROUND: The 1100delC CHEK2 allele has been associated with a 1.4–4.7 fold increased risk for breast cancer in women carrying this mutation. While the frequency of 1100delC was 1.1–1.4% in healthy Finnish controls, the frequency of this allele in a North American control population and in North American breast cancer kindreds remains unclear. METHODS: We genotyped 1665 healthy New York

Kenneth Offit; Heather Pierce; Tomas Kirchhoff; Prema Kolachana; Beth Rapaport; Peter Gregersen; Steven Johnson; Orit Yossepowitch; Helen Huang; Jaya Satagopan; Mark Robson; Lauren Scheuer; Khedoudja Nafa; Nathan Ellis

2003-01-01

471

Variation in mangrove forest structure and sediment characteristics in Bocas del Toro, Panama  

USGS Publications Warehouse

Mangrove forest structure and sediment characteristics were examined in the extensive mangroves of Bocas del Toro, Republic of Panama. Forest structure was characterized to determine if spatial vegetation patterns were repeated over the Bocas del Toro landscape. Using a series of permanent plots and transects we found that the forests of Bocas del Toro were dominated by Rhizophora mangle with very few individuals of Avicennia germinans and Laguncularia racemosa. Despite this low species diversity, there was large variation in forest structure and in edaphic conditions (salinity, concentration of available phosphorus, Eh and sulphide concentration). Aboveground biomass varied 20-fold, from 6.8 Mg ha-1 in dwarf forests to 194.3 Mg ha-1 in the forests fringing the land. But variation in forest structure was predictable across the intertidal zone. There was a strong tree height gradient from seaward fringe (mean tree height 3.9 m), decreasing in stature in the interior dwarf forests (mean tree height 0.7 m), and increasing in stature in forests adjacent to the terrestrial forest (mean tree height 4.1 m). The predictable variation in forest structure emerges due to the complex interactions among edaphic and plant factors. Identifying predictable patterns in forest structure will aid in scaling up the ecosystem services provided by mangrove forests in coastal landscapes. Copyright 2005 College of Arts and Sciences.

Lovelock, C. E.; Feller, I. C.; McKee, K. L.; Thompson, R.

2005-01-01

472

Campano-Maastrichtian foraminifera from onshore sediments in the Rio del Rey Basin, Southwest Cameroon  

NASA Astrophysics Data System (ADS)

Campanian-Maastrichtian marine sediments outcrop in five genetically linked sedimentary basins along the West African coast in the Gulf of Guinea, from the Douala Basin in Cameroon to the Anambra Basin in Nigeria. These sediments in the more centrally located Rio del Rey Basin have been the least studied. Therefore, the geologic history of this region has merely been speculative. The Rio del Rey Basin like the adjacent Niger Delta is producing hydrocarbon from the offshore Tertiary sedimentary interval in which all studies have been focused, neglecting the onshore Cretaceous sediments. Outcrops in the basin are rare, small and highly weathered. Samples from some of these sediments have yielded a few Planktonic and dominantly benthonic foraminiferal assemblages. The long-ranging heterohelix and hedbergellids characterized the planktics while the species Afrobolivina afra which is a well known diagnostic taxon for Campanian-Maastrichtian sediments in West African basins clearly dominate the benthic assemblage. Its occurrence in association with other Upper Cretaceous forms such as Bolivina explicata, Praebulimina exiqua, Gabonita lata, Ammobaculites coprolithiformis amongst others, formed the basis on which this age was assigned to the sediments sampled from the Rio del Rey Basin. Hence, this work has undoubtedly established the much needed link in this regional geologic history and correlates these sediments with the Logbaba and Nkporo Formations in the Douala Basin in Cameroon and the southeastern Nigerian Sedimentary Basins. Thus, these units were all deposited during this same geologic period and probably controlled by the same geologic event.

Njoh, Oliver Anoh; Victor, Obiosio; Christopher, Agyingi

2013-03-01

473

CASP-8 -652 6N ins/del polymorphism and cancer risk: A literature-based systematic HuGE review and meta-analysis  

PubMed Central

Caspase-8 (encoded by the CASP-8 gene) is crucial in generating cell death signals and eliminating potentially malignant cells. Genetic variation in CASP8 may affect susceptibility to cancer. The CASP-8 ?652 6N ins/del (rs3834129) polymorphism has been previously reported to influence the progression to several cancers. However, the overall reported studies have shown inconsistent conclusions. In this human genome epidemiology (HuGE) review and meta-analysis, the aim was to identify the association between CASP-8 ?652 6N ins/del polymorphism and cancer risk. According to the inclusion criteria, 19 case-control studies with a total of 23,172 cancer cases and 26,532 healthy controls were retrieved. Meta-analysis results showed that the del allele, del allele carrier and ins/del genotype of ?652 6N ins/del in the CASP-8 gene were negatively associated with cancer risk (OR=0.91, 95% CI=0.84–0.98, P=0.01; OR=0.88, 95% CI=0.80–0.96, P=0.005; OR=0.91, 95% CI=0.85–0.98, P<0.001; respectively, while no significant correlation was observed between the del/del genotype of ?652 6N ins/del and cancer risk (OR=0.89, 95% CI=0.79–1.01, P=0.08). In the subgroup analysis by ethnicity, the meta-analysis indicated that Caucasian populations harboring the del allele, del allele carriers and ins/del genotype had a lower cancer risk (OR=0.96, 95% CI=0.93–1.00, P=0.05; OR=0.86, 95% CI=0.75–1.00, P=0.05; OR=0.91, 95% CI=0.84–0.98, P=0.01; respectively). In addition, a negative association was found between the del allele of ?652 6N ins/del in the CASP-8 gene and cancer risk in the Asian population (OR=0.89, 95% CI=0.83–0.97, P=0.005). In conclusion, this meta-analysis suggests that the del allele, del allele carrier and ins/del geno-type of the ?652 6N ins/del polymorphism in the CASP-8 gene may be protective factors for cancer risk.

CHEN, DA; MA, TAO; LIU, XIAO-WEI; LIU, ZHI

2012-01-01

474

CASP8 -652 6N del polymorphism and cancer risk: a meta-analysis of 30 case-control studies in 50,112 subjects.  

PubMed

Disruptions of normal apoptotic pathways, which are mainly mediated by caspases, play an essential role in cancer development. Caspase-8 (CASP8) is encoded by the CASP8 gene and is centrally involved in the apoptosis of T lymphocytes. The association between a six-nucleotide deletion polymorphism (-652 6N del) of the CASP8 gene and the risk of cancer is widely reported; however, study results have been inconsistent and contradictory. To evaluate the association between the CASP8 -652 6N del polymorphism and the risk of cancer and to overcome the limitations of any individual study, a meta-analysis based on a total of 23?700 cases and 26?412 controls from 30 case-control studies was conducted. The results of the overall analysis suggested that the CASP8 -652 6N del polymorphism is associated with decreased risk of cancer for the allele contrast [del versus ins: odd ratio (OR) = 0.86, 95% confidence interval (CI) = 0.80-0.92], the additive genetic model (del/del versus ins/ins: OR = 0.78, 95% CI = 0.69-0.88), the dominant genetic model (del/del+del/ins versus ins/ins: OR = 0.83, 95% CI = 0.78-0.89) and the recessive genetic model (del/del versus ins/ins+del/ins: OR = 0.84, 95% CI = 0.75-0.93). In addition, after stratification for ethnicity and cancer type, significantly reduced risk was found for Asians and Caucasians as well as for individuals in the colorectal cancer group and the 'other cancers' group. Accordingly, there is an association between the CASP8 -652 6N del polymorphism and reduced cancer risk, especially among Asians, Caucasians and those with colorectal cancer. However, further research, such as studies focusing on additional ethnic groups and cancer types, is needed to provide a more exact and comprehensive synthesis conclusion. PMID:22513478

Zhang, Fan; Yang, Yuan; Guo, Cheng; Wang, Yang

2012-04-18

475

Prevalence of the BRCA1 c.68_69delAG (BIC: 185delAG) mutation in women with breast cancer from north-central Poland and a review of the literature on other regions of the country  

PubMed Central

Aim of the study Germline mutations in BRCA tumor suppressor genes are strongly associated with breast and ovarian cancer. The lifetime risk of these cancers in women with BRCA1 mutation is 84% and 27%, respectively. Studies on the prevalence of BRCA1 c.68_69delAG congenital mutation, the most frequent in Ashkenazi Jews, among women with breast cancer from north-central Poland and review of the literature on other regions of the country. Evaluation of the c.68_69delAG association with breast cancer risk, with respect to women's age at diagnosis and family history of cancer. Material and methods 252 women with breast cancer, without any of the mutations c.5266dupC, c.181T > G, or c.4034delA, regardless of histological type and family history of cancer. The mutation was detected using allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) assay and confirmed by sequence analysis. Results The c.68_69delAG mutation was disclosed in one out of the 252 women (0.4%), who had been diagnosed with breast cancer at age 43. Family investigations revealed the presence of c.68_69delAG also in the patient's mother, diagnosed with breast cancer at age 68. Sequence analysis confirmed the heterozygous status of the mutation, and family investigation its hereditary character. In the group of families with breast cancer history 1.4% frequency of c.68_69delAG was shown. Conclusions Among families with breast cancer aggregation, originating from north-central Poland, c.68_69delAG is a rare BRCA1 alteration, similarly to other central regions of the country, investigated by other authors. However, in northern, north-western and south-western parts of Poland, it occurs 2–4 times more frequently than in our region.

Janiszewska, Hanna; Bak, Aneta; Pilarska, Maria; Heise, Marta; Junkiert-Czarnecka, Anna; Laskowski, Ryszard; Haus, Olga

2013-01-01

476

A systematic review and meta-analysis of 235delC mutation of GJB2 gene  

PubMed Central

Background The 235delC mutation of GJB2 gene is considered as a risk factor for the non-syndromic hearing loss (NSHL), and a significant difference in the frequency and distribution of the 235delC mutation has been described world widely. Methods A systematic review was performed by means of a meta-analysis to evaluate the influence of the 235delC mutation on the risk of NSHL. A literature search in electronic databases using keywords “235delC”, “GJB2” associated with “carrier frequency” was conducted to include all papers from January 1999 to June 2011. A total of 36 papers were included and there contained 13217 cases and 6521 controls derived from Oceania, American, Europe and Asian. Results A remarkable heterogeneity between these studies was observed. The combined results of meta-analysis showed that the 235delC mutant increased the risk of NSHL (OR?=?7.9, 95%CI 4.77?~?13.11, P <0.00001). Meanwhile, heterogeneity of genetic effect was also observed due to the ethnic specificity and regional disparity. Therefore, the stratified meta-analysis was subsequently conducted and the results indicated that the 235delC mutation was significantly correlated with the risk of NHSL in the East Asian and South-east Asian populations (OR?=?12.05, 95%CI 8.33~17.44, P <0.00001), but not significantly in the Oceania and European populations (OR?=?10.36, 95%CI: 4.68~22.96, Z?=?1.68, P >0.05). Conclusions The 235delC mutation of GJB2 gene increased the risk of NHSL in the East Asian and South-east Asian populations, but non-significantly associated with the NSHL susceptibility in Oceania and European populations, suggesting a significant ethnic specificity of this NSHL-associated mutation.

2012-01-01

477

The E2FD/DEL2 factor is a component of a regulatory network controlling cell proliferation and development in Arabidopsis.  

PubMed

An emerging view of plant cell cycle regulators, including the E2F transcription factors, implicates them in the integration of cell proliferation and development. Arabidopsis encodes six E2F proteins that can act as activators or repressors of E2F-responsive genes. E2FA, E2FB and E2FC interact with the retinoblastoma-like RBR protein and bind to DNA together with their DP partners. In contrast, E2FD, E2FE and E2FF (also known as DEL2, DEL1 and DEL3) are atypical E2Fs that possess duplicated DNA binding regions, lack trans-activating and RBR-binding domains and are believed to act as transcr