Science.gov

Sample records for 14-15 micron two-color

  1. 8-9 and 14-15 Micron Two-Color 640x486 GaAs/AlGaAs Quantum Well Infrared Photodetector (QWIP) Focal Plane Array Camera

    NASA Technical Reports Server (NTRS)

    Gunapala, S. D.; Bandara, S. V.; Singh, A.; Liu, J. K.; Rafol, S. B.; Luong, E. M.; Mumolo, J. M.; Tran, N. Q.; Vincent, J. D.; Shott, C. A.

    2000-01-01

    An optimized long-wavelength two-color Quantum Well Infrared Photodetector (QWIP) device structure has been designed. This device structure was grown on a three inch semi-insulating GaAs substrate by molecular beam epitaxy (MBE). This wafer was processed into several 640x486 format monolithically integrated 8-9 and 14-15 micron two color (or dual wavelength) QWIP focal plane arrays (FPAs). These FPAs were then hybridized to 640x486 silicon CMOS readout multiplexers. A thinned (i.e., substrate removed) FPA hybrid was integrated into a liquid helium cooled dewar to perform electrical and optical characterization and to demonstrate simultaneous two-color imagery. The 8-9 micron detectors in the FPA have shown background limited performance (BLIP) at 70 K operating temperature, at 300 K background with f/2 cold stop. The 14-15 micron detectors of the FPA have reached BLIP at 40 K operating temperature at the same background conditions. In this presentation we discuss the performance of this long-wavelength dualband QWIP FPA in quantum efficiency, detectivity, noise equivalent temperature difference (NEAT), uniformity, and operability.

  2. 8-9 and 14-15 Micron Two-Color 640x486 GaAs/AlGaAs Quantum Well Infrared Photodetector (QWIP) Focal Plane Array Camera

    NASA Technical Reports Server (NTRS)

    Gunapala, S. D.; Bandara, S. V.; Singh, A.; Liu, J. K.; Rafol, S. B.; Luong, E. M.; Mumolo, J. M.; Tran, N. Q.; Vincent, J. D.; Shott, C. A.

    2000-01-01

    An optimized long-wavelength two-color Quantum Well Infrared Photodetector (QWIP) device structure has been designed. This device structure was grown on a three inch semi-insulating GaAs substrate by molecular beam epitaxy (MBE). This wafer was processed into several 640x486 format monolithically integrated 8-9 and 14-15 micron two color (or dual wavelength) QWIP focal plane arrays (FPAs). These FPAs were then hybridized to 640x486 silicon CMOS readout multiplexers. A thinned (i.e., substrate removed) FPA hybrid was integrated into a liquid helium cooled dewar to perform electrical and optical characterization and to demonstrate simultaneous two-color imagery. The 8-9 micron detectors in the FPA have shown background limited performance (BLIP) at 70 K operating temperature, at 300 K background with f/2 cold stop. The 14-15 micron detectors of the FPA have reached BLIP at 40 K operating temperature at the same background conditions. In this presentation we discuss the performance of this long-wavelength dualband QWIP FPA in quantum efficiency, detectivity, noise equivalent temperature difference (NEAT), uniformity, and operability.

  3. 8-9 and 14-15 meu Two-Color 640x486 GaAs/AlGaAs Quantum Well Infrared Photodetector (QWIP) Focal Plane Array Camera

    NASA Technical Reports Server (NTRS)

    Guanapala, S.; Bandara, S.; Singh, A.; Liu, J.; Rafol, S.; Luong, E.; Mumolo, J.; Tran, N.; Vincent, J.; Shott, C.; Long, J.; LeVan, P.

    1999-01-01

    An optimized long-wavelength two-color Quantum Well Infrared Phototdetector (QWIP) device structure has been designed. This device structure was grown on a three-inch semi-insulating GaAs substrate by molecular beam epitaxy (MBE).

  4. 8-9 and 14-15 meu Two-Color 640x486 GaAs/AlGaAs Quantum Well Infrared Photodetector (QWIP) Focal Plane Array Camera

    NASA Technical Reports Server (NTRS)

    Guanapala, S.; Bandara, S.; Singh, A.; Liu, J.; Rafol, S.; Luong, E.; Mumolo, J.; Tran, N.; Vincent, J.; Shott, C.; hide

    1999-01-01

    An optimized long-wavelength two-color Quantum Well Infrared Phototdetector (QWIP) device structure has been designed. This device structure was grown on a three-inch semi-insulating GaAs substrate by molecular beam epitaxy (MBE).

  5. Two-color multiphoton emission from nanotips

    NASA Astrophysics Data System (ADS)

    Cheng-Wei Huang, Wayne; Becker, Maria; Beck, Joshua; Batelaan, Herman

    2017-02-01

    Two-color multiphoton emission from polycrystalline tungsten nanotips has been demonstrated using two-color laser fields. The two-color photoemission is assisted by a three-photon multicolor quantum channel, which leads to a twofold increase in quantum efficiency. Weak-field control of two-color multiphoton emission was achieved by changing the efficiency of the quantum channel with pulse delay. The result of this study complements two-color tunneling photoemission in strong fields, and has potential applications for nanowire-based photonic devices. Moreover, the demonstrated two-color multiphoton emission may be important for realizing ultrafast spin-polarized electron sources via optically injected spin current.

  6. Two-color infrared detector

    DOEpatents

    Klem, John F; Kim, Jin K

    2014-05-13

    A two-color detector includes a first absorber layer. The first absorber layer exhibits a first valence band energy characterized by a first valence band energy function. A barrier layer adjoins the first absorber layer at a first interface. The barrier layer exhibits a second valence band energy characterized by a second valence band energy function. The barrier layer also adjoins a second absorber layer at a second interface. The second absorber layer exhibits a third valence band energy characterized by a third valence band energy function. The first and second valence band energy functions are substantially functionally or physically continuous at the first interface and the second and third valence band energy functions are substantially functionally or physically continuous at the second interface.

  7. Two-color QCD at high density

    SciTech Connect

    Boz, Tamer; Skullerud, Jon-Ivar; Giudice, Pietro; Hands, Simon; Williams, Anthony G.

    2016-01-22

    QCD at high chemical potential has interesting properties such as deconfinement of quarks. Two-color QCD, which enables numerical simulations on the lattice, constitutes a laboratory to study QCD at high chemical potential. Among the interesting properties of two-color QCD at high density is the diquark condensation, for which we present recent results obtained on a finer lattice compared to previous studies. The quark propagator in two-color QCD at non-zero chemical potential is referred to as the Gor’kov propagator. We express the Gor’kov propagator in terms of form factors and present recent lattice simulation results.

  8. Two-Color Laser Speckle Shift Strain Measurement System

    NASA Technical Reports Server (NTRS)

    Tuma, Margaret L.; Krasowski, Michael J.; Oberle, Lawrence G.; Greer, Lawrence C., III; Spina, Daniel; Barranger, John

    1996-01-01

    A two color laser speckle shift strain measurement system based on the technique of Yamaguchi was designed. The dual wavelength light output from an Argon Ion laser was coupled into two separate single-mode optical fibers (patchcords). The output of the patchcords is incident on the test specimen (here a structural fiber). Strain on the fiber, in one direction, is produced using an Instron 4502. Shifting interference patterns or speckle patterns will be detected at real-time rates using 2 CCD cameras with image processing performed by a hardware correlator. Strain detected in fibers with diameters from 21 microns to 143 microns is expected to be resolved to 15 mu epsilon. This system was designed to be compact and robust and does not require surface preparation of the structural fibers.

  9. Wakefield generation via two color laser pulses

    SciTech Connect

    Jha, Pallavi; Saroch, Akanksha; Kumar Verma, Nirmal

    2013-05-15

    The analytical study for the evolution of longitudinal as well as transverse electric wakefields, generated via passage of two color laser pulses through uniform plasma, has been presented in the mildly relativistic regime. The frequency difference between the two laser pulses is assumed to be equal to the plasma frequency, in the present analysis. The relative angle between the directions of polarization of the two laser pulses is varied and the wakefield amplitudes are compared. Further, the amplitude of the excited wakes by two color pulses are compared with those generated by a single laser pulse.

  10. Demonstration of KHILS two-color IR projection capability

    NASA Astrophysics Data System (ADS)

    Jones, Lawrence E.; Coker, Jason S.; Garbo, Dennis L.; Olson, Eric M.; Murrer, Robert Lee, Jr.; Bergin, Thomas P.; Goldsmith, George C., II; Crow, Dennis R.; Guertin, Andrew W.; Dougherty, Michael; Marler, Thomas M.; Timms, Virgil G.

    1998-07-01

    For more than a decade, there has been considerable discussion about using different IR bands for the detection of low contrast military targets. Theory predicts that a target can have little to no contrast against the background in one IR band while having a discernible signature in another IR band. A significant amount of effort has been invested towards establishing hardware that is capable of simultaneously imaging in two IR bands to take advantage of this phenomenon. Focal plane arrays (FPA) are starting to materialize with this simultaneous two-color imaging capability. The Kinetic Kill Vehicle Hardware-in-the-loop Simulator (KHILS) team of the Air Force Research Laboratory and the Guided Weapons Evaluation Facility (GWEF), both at Eglin AFB, FL, have spent the last 10 years developing the ability to project dynamic IR scenes to imaging IR seekers. Through the Wideband Infrared Scene Projector (WISP) program, the capability to project two simultaneous IR scenes to a dual color seeker has been established at KHILS. WISP utilizes resistor arrays to produce the IR energy. Resistor arrays are not ideal blackbodies. The projection of two IR colors with resistor arrays, therefore, requires two optically coupled arrays. This paper documents the first demonstration of two-color simultaneous projection at KHILS. Agema cameras were used for the measurements. The Agema's HgCdTe detector has responsivity from 4 to 14 microns. A blackbody and two IR filters (MWIR equals 4.2 t 7.4 microns, LWIR equals 7.7 to 13 microns) were used to calibrate the Agema in two bands. Each filter was placed in front of the blackbody one at a time, and the temperature of the blackbody was stepped up in incremental amounts. The output counts from the Agema were recorded at each temperature. This calibration process established the radiance to Agema output count curves for the two bands. The WISP optical system utilizes a dichroic beam combiner to optically couple the two resistor arrays. The

  11. Two-color interface vortex solitons

    SciTech Connect

    Xu Zhiyong

    2010-02-15

    We study the existence and properties of vortex solitons supported by an interface between two distinct optical lattices imprinted in nonlinear quadratic media. We analyze the impact of guiding parameters of lattices and phase mismatching conditions on the existence and stability of two-color interface vortex solitons. The salient point is that interface vortex solitons feature highly asymmetric profiles, and are stable throughout almost the entire existence domain.

  12. Two-color walking Peregrine solitary waves.

    PubMed

    Baronio, Fabio; Chen, Shihua; Mihalache, Dumitru

    2017-09-15

    We study the extreme localization of light, evolving upon a non-zero background, in two-color parametric wave interaction in nonlinear quadratic media. We report the existence of quadratic Peregrine solitary waves, in the presence of significant group-velocity mismatch between the waves (or Poynting vector beam walk-off), in the regime of cascading second-harmonic generation. This finding opens a novel path for the experimental demonstration of extreme rogue waves in ultrafast quadratic nonlinear optics.

  13. Millimeter accuracy satellites for two color ranging

    NASA Technical Reports Server (NTRS)

    Degnan, John J.

    1993-01-01

    The principal technical challenge in designing a millimeter accuracy satellite to support two color observations at high altitudes is to provide high optical cross-section simultaneously with minimal pulse spreading. In order to address this issue, we provide, a brief review of some fundamental properties of optical retroreflectors when used in spacecraft target arrays, develop a simple model for a spherical geodetic satellite, and use the model to determine some basic design criteria for a new generation of geodetic satellites capable of supporting millimeter accuracy two color laser ranging. We find that increasing the satellite diameter provides: a larger surface area for additional cube mounting thereby leading to higher cross-sections; and makes the satellite surface a better match for the incoming planar phasefront of the laser beam. Restricting the retroreflector field of view (e.g. by recessing it in its holder) limits the target response to the fraction of the satellite surface which best matches the optical phasefront thereby controlling the amount of pulse spreading. In surveying the arrays carried by existing satellites, we find that European STARLETTE and ERS-1 satellites appear to be the best candidates for supporting near term two color experiments in space.

  14. Optimum wavelengths for two color ranging

    NASA Technical Reports Server (NTRS)

    Degnan, John J.

    1993-01-01

    The range uncertainties associated with the refractive atmosphere can be mitigated by the technique of two color, or dual wavelength, ranging. The precision of the differential time of flight (DTOF) measurement depends on the atmospheric dispersion between the two wavelengths, the received pulsewidths and photoelectron counts, and on the amount of temporal averaging. In general, the transmitted wavelengths are not independently chosen but instead are generated via nonlinear optics techniques (harmonic crystals, Raman scattering, etc.) which also determine their relative pulsewidths. The mean received photoelectrons at each wavelength are calculated via the familiar radar link equation which contains several wavelength dependent parameters. By collecting the various wavelength dependent terms, one can define a wavelength figure of merit for a two color laser ranging system. In this paper, we apply the wavelength figure of merit to the case of an extremely clear atmosphere and draw several conclusions regarding the relative merits of fundamental-second harmonic, fundamental-third harmonic, second-third harmonic, and Raman two color systems. We find that, in spite of the larger dispersion between wavelengths, fundamental-third harmonic systems have the lowest figure of merit due to a combination of poor detector performance at the fundamental and poor atmospheric transmission at the third harmonic. The fundamental-second harmonic systems (approximately 700 nm and 350 nm) have the highest figure of merit, but second-third harmonic systems, using fundamental transmitters near 1000 nm, are a close second. Raman-shifted transmitters appear to offer no advantage over harmonic systems because of the relatively small wavelength separation that can be achieved in light gases such as hydrogen and the lack of good ultrashort pulse transmitters with an optimum fundamental wavelength near 400 nm.

  15. Two-color bright squeezed vacuum

    SciTech Connect

    Agafonov, Ivan N.; Chekhova, Maria V.

    2010-07-15

    In a strongly pumped nondegenerate traveling-wave optical parametric amplifier, we produce a two-color squeezed vacuum with up to millions of photons per pulse. Our approach to registering this macroscopic quantum state is direct detection of a large number of transverse and longitudinal modes, which is achieved by making the detection time and area much larger than the coherence time and area, respectively. Using this approach, we obtain a record value of twin-beam squeezing for direct detection of bright squeezed vacuum. This makes direct detection of macroscopic squeezed vacuum a practical tool for quantum information applications.

  16. Two color holographic interferometry for microgravity application

    NASA Technical Reports Server (NTRS)

    Trolinger, James D.

    1993-01-01

    Holographic interferometry is a primary candidate for the measurement of temperature and concentration in various crystal growth experiments destined for space. The method measures refractive index changes in the experiment test cell. A refractive index change can be caused by concentration changes, temperature changes, or a combination of temperature and concentration changes. If the refractive index changes are caused by temperature and concentration changes occurring simultaneously in the experiment test cell, the contributions by the two effects cannot be separated by conventional measurement methods. By using two wavelengths, two independent interferograms can be produced from the reconstruction of the hologram. The two interferograms will be different due to dispersion properties of fluid materials. These differences provide the additional information that allows the separation of simultaneously occurring temperature and concentration gradients. There is no other technique available that can provide this type of information. The primary objectives of this effort are to experimentally verify the mathematical theory of two color holographic interferometry and to determine the practical value of this technique for space application. To achieve these objectives, the accuracy and sensitivity of the technique must be determined for geometry's and materials that are relevant to the Materials Processing in the Space program of NASA. This will be achieved through the use of a specially designed two-color holographic interferometry breadboard optical system. In addition to experiments to achieve the primary goals, the breadboard will also provide inputs to the design of an optimum space flight system.

  17. A Two Color Quadrature Interferometer for the TCS Experiment

    NASA Astrophysics Data System (ADS)

    Crawford, Edward; Euripides, Peter; Votroubek, George

    1999-11-01

    The TCS experiment at the Redmond Plasma Physics Labotatory (RPPL) is designed to demonstrate current sustainment and heating of a pre-formed FRC plasma using a rotating magnetic field (RMF)at 150 kHz. NE must be accurately measured between 10^19 and 10^21 m-3. We have constructed a two-color, double pass interferometer for the TCS sustainment chamber. CO2 and HeNe lasers at 10.6 and 0.63 microns are used with separate Bragg modulators to generate reference beams shifted by 40 MHz. A unique design uses a single large spherical mirror to focus all beams. We find this technique greatly facilitates alignment. The scene beams may be switched from a single table mode of operation to a through-plasma mode by insertion of a single right angle mirror. Scene beams return to the main table from a hollow corner-cube reflector insuring good signal amplitude stability even though the supporting structure is quite flexible.

  18. Two color holographic interferometry for microgravity application

    NASA Technical Reports Server (NTRS)

    Trolinger, James D.; Weber, David C.

    1995-01-01

    Holographic interferometry is a primary candidate for determining temperature and concentration in crystal growth experiments designed for space. The method measures refractive index changes within the fluid of an experimental test cell resulting from temperature and/or concentration changes. When the refractive index changes are caused by simultaneous temperature and concentration changes, the contributions of the two effects cannot be separated by single wavelength interferometry. By using two wavelengths, however, two independent interferograms can provide the additional independent equation required to determine the two unknowns. There is no other technique available that provides this type of information. The primary objectives of this effort were to experimentally verify the mathematical theory of two color holographic interferometry (TCHI) and to determine the practical value of this technique for space application. In the foregoing study, the theory of TCHI has been tested experimentally over a range of interest for materials processing in space where measurements of temperature and concentration in a solution are required. New techniques were developed and applied to stretch the limits beyond what could be done with existing procedures. The study resulted in the production of one of the most advanced, enhanced sensitivity holographic interferometers in existence. The interferometric measurements made at MSFC represent what is believed to be the most accurate holographic interferometric measurements made in a fluid to date. The tests have provided an understanding of the limitations of the technique in practical use.

  19. Storage ring two-color free-electron laser

    DOE PAGES

    Yan, J.; Hao, H.; Li, J. Y.; ...

    2016-07-05

    We report a systematic experimental study of a storage ring two-color free-electron laser (FEL) operating simultaneously in the infrared (IR) and ultraviolet (UV) wavelength regions. The two-color FEL lasing has been realized using a pair of dual-band high-reflectivity FEL mirrors with two different undulator configurations. We have demonstrated independent wavelength tuning in a wide range for each lasing color, as well as harmonically locked wavelength tuning when the UV lasing occurs at the second harmonic of the IR lasing. Precise power control of two-color lasing with good power stability has also been achieved. In addition, the impact of the degradationmore » of FEL mirrors on the two-color FEL operation is reported. Moreover, we have investigated the temporal structures of the two-color FEL beams, showing simultaneous two-color micropulses with their intensity modulations displayed as FEL macropulses.« less

  20. Development of a two-color FQI

    SciTech Connect

    Butcher, T.; Wei, G.

    1996-07-01

    The Flame Quality Indicator (FQI) concept was developed at Brookhaven National Laboratory as a simple device which could be used to monitor oil burner flames and indicate when a problem was starting to occur. Fault situations which could be identified by the FQI include: fouled nozzle, increased or decreased excess air, blocked air inlet or flue, and use of low quality oil. The basic concept of the FQI is quite simple. A conventional cadmium sulfide photocell is used to measure the amount of light emitted from an oil burner flame when the appliance is fully warmed-up. The measured amount of light is compared to a set point, established during burner tune-up. If the two intensities differ by more than a set range, a {open_quotes}service required{close_quotes} signal is produced. The amount of light which is emitted from an oil burner flame depends upon the amount of {open_quotes}soot{close_quotes} or carbon in the flame, the size and shape of the flame, and the flame temperature. The quality of flame is practically judged by the amount of soot which it is producing and for this reason it is necessary to eliminate effects of other parameters. Temperature is expected to be the most important of these. The FQI eliminates effects due to the chamber environment by establishing a set point for each specific appliance. The transient temperature effects are accounted for by examining the flame brightness only at a single time during the firing cycle. BNL is currently involved with the development of a two-color approach to the monitoring of flame quality. The basic concept involved is the measurement of both flame temperature and total amount of light emitted to allow a more direct estimate to be made of the amount of soot being produced and so the flame quality. The objective is to develop a more sensitive measurement which may be more universally applicable. This paper provides a summary of our approach and results to date in this project.

  1. 15 CFR 14.15 - Metric system of measurement.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Metric system of measurement. 14.15 Section 14.15 Commerce and Foreign Trade Office of the Secretary of Commerce UNIFORM ADMINISTRATIVE... system is the preferred measurement system for U.S. trade and commerce. The Act requires each...

  2. 15 CFR 14.15 - Metric system of measurement.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 1 2013-01-01 2013-01-01 false Metric system of measurement. 14.15... COMMERCIAL ORGANIZATIONS Pre-Award Requirements § 14.15 Metric system of measurement. The Metric Conversion... system is the preferred measurement system for U.S. trade and commerce. The Act requires each Federal...

  3. 15 CFR 14.15 - Metric system of measurement.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 1 2012-01-01 2012-01-01 false Metric system of measurement. 14.15... COMMERCIAL ORGANIZATIONS Pre-Award Requirements § 14.15 Metric system of measurement. The Metric Conversion... system is the preferred measurement system for U.S. trade and commerce. The Act requires each Federal...

  4. 15 CFR 14.15 - Metric system of measurement.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Metric system of measurement. 14.15... COMMERCIAL ORGANIZATIONS Pre-Award Requirements § 14.15 Metric system of measurement. The Metric Conversion... system is the preferred measurement system for U.S. trade and commerce. The Act requires each Federal...

  5. 15 CFR 14.15 - Metric system of measurement.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Metric system of measurement. 14.15... COMMERCIAL ORGANIZATIONS Pre-Award Requirements § 14.15 Metric system of measurement. The Metric Conversion... system is the preferred measurement system for U.S. trade and commerce. The Act requires each Federal...

  6. Two-color double exposure photographic recording technique

    NASA Technical Reports Server (NTRS)

    Stricker, Josef

    1989-01-01

    A two-color double exposure technique is proposed for recording two close states of an object on the same photographic film. The idea is to use a two-color laser for illumination, one color for each state of the object, and a single photographic plate. After being developed, the image of each state may be observed separately. The technique is demonstrated for the deferred moire deflectometry where two closely displaced fringe patterns have to be recorded and resolved.

  7. 50 CFR 14.15 - Personal baggage and household effects.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 1 2012-10-01 2012-10-01 false Personal baggage and household effects. 14... Designated Ports § 14.15 Personal baggage and household effects. (a) Any person may import into or export..., or crusted hide or skin; game trophy; or to wildlife requiring a permit pursuant to part 16, 17,...

  8. 50 CFR 14.15 - Personal baggage and household effects.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 1 2013-10-01 2013-10-01 false Personal baggage and household effects. 14... Designated Ports § 14.15 Personal baggage and household effects. (a) Any person may import into or export..., or crusted hide or skin; game trophy; or to wildlife requiring a permit pursuant to part 16, 17,...

  9. 50 CFR 14.15 - Personal baggage and household effects.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 1 2010-10-01 2010-10-01 false Personal baggage and household effects. 14... Designated Ports § 14.15 Personal baggage and household effects. (a) Any person may import into or export..., or crusted hide or skin; game trophy; or to wildlife requiring a permit pursuant to part 16, 17,...

  10. 50 CFR 14.15 - Personal baggage and household effects.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 1 2014-10-01 2014-10-01 false Personal baggage and household effects. 14... Designated Ports § 14.15 Personal baggage and household effects. (a) Any person may import into or export..., or crusted hide or skin; game trophy; or to wildlife requiring a permit pursuant to part 16, 17,...

  11. Two-Color Ultrafast Photoexcited Scanning Tunneling Microscopy

    SciTech Connect

    Camillone, N.; Dolocan, A.; Acharya, D.P.; Zahl, P.; Sutter, P.

    2011-05-26

    We report on two-color two-photon photoexcitation of a metal surface driven by ultrafast laser pulses and detected with a scanning tunneling microscope (STM) tip as a proximate anode. Results are presented for two cases: (i) where the tip is retracted from the surface far enough to prohibit tunneling, and (ii) where the tip is within tunneling range of the surface. A delay-modulation technique is implemented to isolate the two-color photoemission from concurrent one-color two-photon photoemission and provide subpicosecond time-resolved detection. When applied with the tip in tunneling range, this approach effectively isolates the two-photon photoexcited current signal from the conventional tunneling current and enables subpicosecond time-resolved detection of the photoexcited surface electrons. The advantage of the two-color approach is highlighted by comparison with the one-color case where optical interference causes thermal modulation of the STM tip length, resulting in tunneling current modulations that are orders of magnitude larger than the current due to photoexcitation of surface electrons. By completely eliminating this interference, and thereby avoiding thermal modulation of the STM tip length, the two-color approach represents an important step toward the ultimate goal of simultaneous subnanometer and subpicosecond measurements of surface electron dynamics by ultrafast-laser-excited STM.

  12. Near-infrared two-color pyrometer for determining ignition temperatures of metals and metal alloys

    NASA Technical Reports Server (NTRS)

    Nguyen, K.; Branch, M. C.

    1985-01-01

    A two-color pyrometer has been designed, constructed, and used to measure the ignition temperatures of metals and metal alloys. Cylindrical metal and metal alloy specimens were ignited by a focused cw CO2 laser beam in a cool, static, pure oxygen environment. The pyrometer operates in the near-infrared at two narrow spectral regions, with a nominal bandwidth of 10 nm centered at 0.9051 and 1.06 micron, and has a temperature range from 1000 to 4000 K. In the present design the temperature of a spot, about 0.5 mm in diameter, can be recorded with a maximum time resolution of 25 microseconds and with an accuracy of a few percent. Results of CO2 laser ignition of cylindrical specimens of 6061 aluminum alloy and 302 SS in a pure oxygen environment were obtained from the two-color pyrometer and were compared with those obtained from a thermocouple placed inside the specimen near the laser-irradiated surface.

  13. Tunneling ionization and harmonic generation in two-color fields

    NASA Astrophysics Data System (ADS)

    Kondo, K.; Kobayashi, Y.; Sagisaka, A.; Nabekawa, Y.; Watanabe, S.

    1996-02-01

    Tunneling ionization and harmonic generation in two-color fields were studied with a fundamental beam ( omega ) and its harmonics (2 omega ,3 omega ) , which were generated by a 100-fs Ti:sapphire laser. Ion yields of atoms and molecules were successfully controlled by means of a change in the relative phase between omega and 3 omega pulses. Two-color interference was clearly observed in photoelectron spectra and harmonic spectra. In the omega - 2 omega field even-order harmonics were observed in which the intensity was almost equal to that of the odd harmonics because of an asymmetric optical field. These results were compared with the quasi-static model for ionization and with the quantum theory for harmonic generation.

  14. Two-Color Laser Resonance Ionization Spectroscopy of Zirconium Atoms

    NASA Astrophysics Data System (ADS)

    Hasegawa, Shuichi; Nagamoto, Daisuke

    2017-10-01

    We have performed two-color laser resonance ionization spectroscopy of zirconium atoms to measure the energies of excited states below the third ionization limit. The number of intermediate states that we observed is 19, and energies deduced from the experiments agree with previous data. Complex ionization spectra of the excited states were observed through the intermediate states. The values of the first, second, and third ionization limits were derived from the Rydberg series of the spectra with quantum defect theory.

  15. Design study for a two-color beta measurement system

    NASA Technical Reports Server (NTRS)

    1982-01-01

    Design analysis of the beam splitter combined two color beta system is presented. Conventional and dichroic beam splitters are discussed. Design analysis of the beta system employing two beams with focusing at separate points is presented. Alterations and basic parameters of the two beam system are discussed. Alterations in the focus of the initial laser and the returning beams are also discussed. Heterodyne efficiencies for the on axis and off axis reflected radiation are included.

  16. Statistical designs for two-color spotted microarray experiments.

    PubMed

    Chai, Feng-Shun; Liao, Chen-Tuo; Tsai, Shin-Fu

    2007-04-01

    Two-color cDNA or oligonucleotide-based spotted microarrays have been commonly used in measuring the expression levels of thousands of genes simultaneously. To realize the immense potential of this powerful new technology, budgeted within limited resources or other constraints, practical designs with high efficiencies are in demand. In this study, we address the design issue concerning the arrangement of the mRNA samples labeled with fluorescent dyes and hybridized on the slides. A normalization model is proposed to characterize major sources of systematic variation in a two-color microarray experiment. This normalization model establishes a connection between designs for two-color microarray experiments with a particular class of classical row-column designs. A heuristic algorithm for constructing A-optimal or highly efficient designs is provided. Statistical optimality results are found for some of the designs generated from the algorithm. It is believed that the constructed designs are the best or very close to the best possible for estimating the relative gene expression levels among the mRNA samples of interest.

  17. Physical mechanism of terahertz generation in two-color photoionization

    NASA Astrophysics Data System (ADS)

    You, Yong Sing

    Two-color photoionization has been widely used as a versatile tool for intense, broadband terahertz (THz) radiation generation. In this scheme, an ultrashort laser's fundamental and its second harmonic pulses are co-focused into a gas of atoms or molecules, transforming them into plasma by photoionization. This process produces an intense THz pulse emitted in the forward direction. The main focus of this dissertation is to provide a physical understanding of such THz generation and investigate its generation mechanism at both microscopic and macroscopic levels. First, we examine the generation process by measuring the relative phase between two-color (fundamental and second harmonic) laser fields and the resulting THz field simultaneously. We discover that a relative phase of pi/2 yields maximal THz outputs, consistent with a semi-classical plasma current model. We find that this optimal relative phase is independent of laser intensities, gas species, and two-color laser amplitude ratios. We also measure concurrent near-field photocurrents. All these measurements verify laser-produced plasma currents as a microscopic source for THz generation. We also investigate THz radiation from an ensemble of aligned air molecules in two-color laser fields. Our experiments show that THz radiation is strongly affected by molecular (nitrogen and oxygen) alignment. We explain this phenomenon in the context of the plasma current model combined with alignment-dependent ionization. Phase-matching is essential to achieve high-efficiency nonlinear frequency conversion. We discover THz generation by two-color photoionization in elongated air plasmas (filamentation) is naturally phase-matched in the off-axis direction, resulting in donut-shaped radiation profiles in the far field. Because of this off-axis phase-matching, THz yields increase almost linearly with the filament length, scalable for further THz energy enhancement. Lastly, we study the polarization of emitted THz radiation. In

  18. Characterization of 14,15-epoxyeicosatrienoyl-sulfonamides as 14,15-epoxyeicosatrienoic acid agonists: use for studies of metabolism and ligand binding.

    PubMed

    Yang, Wenqi; Holmes, Blythe B; Gopal, V Raj; Kishore, R V Krishna; Sangras, Bhavani; Yi, Xiu-Yu; Falck, J R; Campbell, William B

    2007-06-01

    Epoxyeicosatrienoic acids (EETs) are cytochrome P450 epoxygenase metabolites of arachidonic acid. EETs mediate numerous biological functions. In coronary arteries, they regulate vascular tone by the activation of smooth muscle large-conductance, calcium-activated potassium (BK(Ca)) channels to cause hyperpolarization and relaxation. We developed a series of 14,15-EET agonists, 14,15-EET-phenyliodosulfonamide (14,15-EET-PISA), 14,15-EET-biotinsulfonamide (14,15-EET-BSA), and 14,15-EET-benzoyldihydrocinnamide-sulfonamide (14,15-EET-BZDC-SA) as tools to characterize 14,15-EET metabolism and binding. Agonist activities of these analogs were characterized in precontraced bovine coronary arterial rings. All three analogs induced concentration-dependent relaxation and were equipotent with 14,15-EET. Relaxations to these analogs were inhibited by the BK(Ca) channel blocker iberiotoxin (100 nM), the 14,15-EET antagonist 14,15-epoxyeicosa-5(Z)-enoylmethylsulfonamide (10 muM), and abolished by 20 mM extracellular K(+). 14,15-EET-PISA is metabolized to 14,15-dihydroxyeicosatrienoyl-PISA by soluble epoxide hydrolase in bovine coronary arteries and U937 cells but not U937 cell membrane fractions. 14,15-EET-P(125)ISA binding to human U937 cell membranes was time-dependent, concentration-dependent, and saturable. The specific binding reached equilibrium by 15 min at 4 degrees C and remained unchanged up to 30 min. The estimated K(d) and B(max) were 148.3 +/- 36.4 nM and 3.3 +/- 0.5 pmol/mg protein, respectively. These data suggest that 14,15-EET-PISA, 14,15-EET-BSA, and 14,15-EET-BZDC-SA are full 14,15-EET agonists. 14,15-EET-P(125)ISA is a new radiolabeled tool to study EET metabolism and binding. Our results also provide preliminary evidence that EETs exert their biological effect through a membrane binding site/receptor.

  19. Two-color pyrometry for low amplitude periodic heating

    NASA Astrophysics Data System (ADS)

    Bennett, T. D.; Silveira, V. B.; Valdes, R.

    2017-02-01

    Specimens subject to periodic heating must be probed for a calibrated temperature response if standard measurements of thermal diffusivity are to be extended to determine thermal conductivity. A variation on two-color pyrometry is developed to measure both the offset and harmonic amplitudes of temperature fluctuations caused by periodic heating. The requisite pyrometric formulae are derived for low amplitude heating using an expansion of the nonlinear thermal emission. Well-defined uncertainties in the temperature values are determined from experimental uncertainties in radiometric measurements. The accuracy demonstrated in this work is better than 2% for the temperature offset and 3%-8% for the fluctuating temperature amplitude.

  20. Two-Step Filament Eruption During 14 - 15 March 2015

    NASA Astrophysics Data System (ADS)

    Chandra, R.; Filippov, B.; Joshi, R.; Schmieder, B.

    2017-06-01

    We present here an interesting two-step filament eruption during 14 - 15 March 2015. The filament was located in NOAA AR 12297 and associated with a halo Coronal Mass Ejection (CME). We use observations from the Atmospheric Imaging Assembly (AIA) and Heliospheric Magnetic Imager (HMI) instruments onboard the Solar Dynamics Observatory (SDO), and from the Solar and Heliospheric Observatory (SOHO) Large Angle and Spectrometric Coronagraph (LASCO). We also use Hα data from the Global Oscillation Network Group (GONG) telescope and the Kanzelhoehe Solar Observatory. The filament shows a first step eruption on 14 March 2015 and it stops its rise at a projected altitude {≈} 125 Mm on the solar disk. It remains at this height for {≈} 12 hrs. Finally it erupts on 15 March 2015 and produces a halo CME. We also find jet activity in the active region during both days, which could help the filament de-stabilization and eruption. The decay index is calculated to understand this two-step eruption. The eruption could be due to the presence of successive instability-stability-instability zones as the filament is rising.

  1. Silicon photodiode as the two-color detector

    NASA Astrophysics Data System (ADS)

    Ponomarev, D. B.; Zakharenko, V. A.

    2015-11-01

    This paper describes a silicon photodiode as the two-color photodetector. The work of one photodiode in two spectral ranges is achieved due to the changes of the spectral sensitivity of the photodiodes in the transition from photodiode mode for photovoltaic in the short circuit mode. On the basis of silicon photodiode FD-256 the layout of the spectral ratio pyrometer was assembled and the results of theoretical calculations was confirmed experimentally. The calculated dependences of the coefficient of error of the spectral ratio pyrometer from temperature reverse voltage 10 and 100 V was presented. The calculated dependence of the instrumental error and the assessment of methodological errors of the proposed photodetector spectral ratio was done. According to the results of the presented research was set the task of development photodiode detectors which change the spectral sensitivity depending on the applied voltage.

  2. Two-color ghost imaging with enhanced angular resolving power

    SciTech Connect

    Karmakar, Sanjit; Shih, Yanhua

    2010-03-15

    This article reports an experimental demonstration on nondegenerate, two-color, biphoton ghost imaging which reproduced a ghost image with enhanced angular resolving power by means of a greater field of view compared with that of classical imaging. With the same imaging magnification, the enhanced angular resolving power and field of view compared with those of classical imaging are 1.25:1 and 1.16:1, respectively. The enhancement of angular resolving power depends on the ratio between the idler and the signal photon frequencies, and the enhancement of the field of view depends mainly on the same ratio and also on the distances of the object plane and the imaging lens from the two-photon source. This article also reports the possibility of reproducing a ghost image with the enhancement of the angular resolving power by means of a greater imaging amplification compared with that of classical imaging.

  3. Voltage tunable two-color infrared detection using semiconductor superlattices.

    SciTech Connect

    Majumdar, Amlan; Choi, Kyung K.; Tsui, Daniel Chee; Reno, John Louis

    2003-08-01

    We demonstrate a voltage tunable two-color quantum-well infrared photodetector (QWIP) that consists of multiple periods of two distinct AlGaAs/GaAs superlattices separated by AlGaAs blocking barriers on one side and heavily doped GaAs layers on the other side. The detection peak switches from 9.5 {micro}m under large positive bias to 6 {micro}m under negative bias. The background-limited temperature is 55 K for 9.5 {micro}m detection and 80 K for 6 {micro}m detection. We also demonstrate that the corrugated-QWIP geometry is suitable for coupling normally incident light into the detector.

  4. Two-color detection with charge sensitive infrared phototransistors

    NASA Astrophysics Data System (ADS)

    Kim, Sunmi; Komiyama, Susumu; Ueda, Takeji; Satoh, Takashi; Kajihara, Yusuke

    2015-11-01

    Highly sensitive two-color detection is demonstrated at wavelengths of 9 μm and 14.5 μm by using a charge sensitive infrared phototransistor fabricated in a triple GaAs/AlGaAs quantum well (QW) crystal. Two differently thick QWs (7 nm- and 9 nm-thicknesses) serve as photosensitive floating gates for the respective wavelengths via intersubband excitation: The excitation in the QWs is sensed by a third QW, which works as a conducting source-drain channel in the photosensitive transistor. The two spectral bands of detection are shown to be controlled by front-gate biasing, providing a hint for implementing voltage tunable ultra-highly sensitive detectors.

  5. Two-color beam generation based on wakefield excitation

    NASA Astrophysics Data System (ADS)

    Bettoni, S.; Prat, E.; Reiche, S.

    2016-05-01

    Several beam manipulation methods have been studied and experimentally tested to generate two-color photon beams in free electron laser facilities to accommodate the user requests. We propose to use the interaction of the beam with an oscillating longitudinal wakefield source to obtain a suitable electron beam structure. The bunch generates two subpulses with different energies and delayed in time passing through a magnetic chicane after its longitudinal phase space has been modulated by the wakefield source. According to this approach the power of the emitted radiation is not degraded compared to the monochromatic beam, and the setup in the machine is quite simple because the bunch is manipulated only in the high energy section, where it is more rigid. We present the design applied to SwissFEL. We identified the parameters and the corresponding range of tunability of the time and energy separation among the two subbunches.

  6. Multidimensional data reconstruction for two color fluorescence microscopy

    NASA Astrophysics Data System (ADS)

    Dilipkumar, Shilpa; Mondal, Partha Pratim

    2012-12-01

    We propose an iterative data reconstruction technique specifically designed for multi-dimensional multi-color fluorescence imaging. Markov random field is employed (for modeling the multi-color image field) in conjunction with the classical maximum likelihood method. It is noted that, ill-posed nature of the inverse problem associated with multi-color fluorescence imaging forces iterative data reconstruction. Reconstruction of three-dimensional (3D) two-color images (obtained from nanobeads and cultured cell samples) show significant reduction in the background noise (improved signal-to-noise ratio) with an impressive overall improvement in the spatial resolution (≈250 nm) of the imaging system. Proposed data reconstruction technique may find immediate application in 3D in vivo and in vitro multi-color fluorescence imaging of biological specimens.

  7. Two color laser driven THz generation in clustered plasma

    NASA Astrophysics Data System (ADS)

    Malik, Rakhee; Uma, R.; Kumar, Pawan

    2017-07-01

    A scheme of terahertz (THz) generation, using nonlinear mixing of two color laser (fundamental ω1 and slightly frequency shifted second harmonic ω2 ) in clustered plasma, is investigated. The lasers exert ponderomotive force on cluster electrons and drive density perturbations at 2 ω1 and ω2-ω1 . The density perturbations beat with the oscillatory velocities to produce nonlinear current at ω2-2 ω1 , generating THz radiation. The radiation is enhanced due to cluster plasmon resonance and by phase matching introduced through a density ripple. The generation involves third order nonlinearity and does not require a magnetic field or inhomogeneity to sustain it. We report THz power conversion efficiency ˜ 10-4 at 1 μm and 0.5 μm wavelengths with intensity ˜ 3 ×1014W/cm 2 .

  8. Two-color detection with charge sensitive infrared phototransistors

    SciTech Connect

    Kim, Sunmi Kajihara, Yusuke; Komiyama, Susumu; Ueda, Takeji; Satoh, Takashi

    2015-11-02

    Highly sensitive two-color detection is demonstrated at wavelengths of 9 μm and 14.5 μm by using a charge sensitive infrared phototransistor fabricated in a triple GaAs/AlGaAs quantum well (QW) crystal. Two differently thick QWs (7 nm- and 9 nm-thicknesses) serve as photosensitive floating gates for the respective wavelengths via intersubband excitation: The excitation in the QWs is sensed by a third QW, which works as a conducting source-drain channel in the photosensitive transistor. The two spectral bands of detection are shown to be controlled by front-gate biasing, providing a hint for implementing voltage tunable ultra-highly sensitive detectors.

  9. Two-color hybrid laser wakefield and direct laser accelerator

    NASA Astrophysics Data System (ADS)

    Zhang, Xi; Khudik, V.; Bernstein, A.; Downer, M.; Shvets, G.

    2017-03-01

    We propose and investigate the concept of two-color laser wakefield and direct acceleration (LWDA) scheme in the regime of moderate (10 TW scale) laser powers. The concept utilizes two unequal frequency laser pulses: the leading long-wavelength (λ0 = 0.8 µm) wakefield laser pulse driving a nonlinear plasma wake, and a trailing short-wavelength (λDLA = λ0/2) DLA laser pulse. The combination of the large electric field, yet small ponderomotive pressure of the DLA pulse is shown to be advantageous for producing a higher energy and larger charge electron beam compared with the single frequency LWDA. The sensitivity of the dual-frequency LWDA to synchronization time jitter is also reduced.

  10. Two-color holography concept (T-CHI)

    NASA Technical Reports Server (NTRS)

    Vikram, C. S.; Caulfield, H. J.; Workman, G. L.; Trolinger, J. D.; Wood, C. P.; Clark, R. L.; Kathman, A. D.; Ruggiero, R. M.

    1990-01-01

    The Material Processing in the Space Program of NASA-MSFC was active in developing numerous optical techniques for the characterization of fluids in the vicinity of various materials during crystallization and/or solidification. Two-color holographic interferometry demonstrates that temperature and concentration separation in transparent (T-CHI) model systems is possible. The experiments were performed for particular (succinonitrile) systems. Several solutions are possible in Microgravity Sciences and Applications (MSA) experiments on future Shuttle missions. The theory of the T-CHI concept is evaluated. Although particular cases are used for explanations, the concepts developed will be universal. A breadboard system design is also presented for ultimate fabrication and testing of theoretical findings. New developments in holography involving optical fibers and diode lasers are also incorporated.

  11. 10 CFR 14.15 - Where to present a claim to NRC.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Where to present a claim to NRC. 14.15 Section 14.15 Energy NUCLEAR REGULATORY COMMISSION ADMINISTRATIVE CLAIMS UNDER FEDERAL TORT CLAIMS ACT Filing Procedures and Requirements § 14.15 Where to present a claim to NRC. A claimant shall mail or deliver the...

  12. A relative-intensity two-color phosphor thermography system

    NASA Technical Reports Server (NTRS)

    Merski, N. Ronald

    1991-01-01

    The NASA LaRC has developed a relative-intensity two-color phosphor thermography system. This system has become a standard technique for acquiring aerothermodynamic data in LaRC Hypersonic Facilities Complex (HFC). The relative intensity theory and its application to the LaRC phosphor thermography system is discussed along with the investment casting technique which is critical to the utilization of the phosphor method for aerothermodynamic studies. Various approaches to obtaining quantitative heat transfer data using thermographic phosphors are addressed and comparisons between thin-film data and thermographic phosphor data on an orbiter-like configuration are presented. In general, data from these two techniques are in good agreement. A discussion is given on the application of phosphors to integration heat transfer data reduction techniques (the thin film method) and preliminary heat transfer data obtained on a calibration sphere using thin-film equations are presented. Finally, plans for a new phosphor system which uses target recognition software are discussed.

  13. Two-color excited-state absorption imaging of melanins

    NASA Astrophysics Data System (ADS)

    Fu, Dan; Ye, Tong; Matthews, Thomas E.; Yurtsever, Gunay; Hong, Lian; Simon, John D.; Warren, Warren S.

    2007-02-01

    We have demonstrated a new method for imaging melanin with two-color excited state absorption microscopy. If one of two synchronized mode-locked pulse trains at different colors is intensity modulated, the modulation transfers to the other pulse train when nonlinear absorption takes place in the medium. We can easily measure 10 -6 absorption changes caused by either instantaneous two-photon absorption or relatively long lived excited state absorption with a RF lock-in amplifier. Eumelanin and pheomelanin exhibit similar excited state dynamics. However, their difference in excited state absorption and ground state absorption leads to change in the phase of the transient absorption signal. Scanning microscopic imaging is performed with B16 cells, melanoma tissue to demonstrate the 3D high resolution imaging capability. Different melanosome samples are also imaged to illustrate the differences between eumelanin and pheomelanin signals. These differences could enable us to image their respective distribution in tissue samples and provide us with valuable information in diagnosing malignant transformation of melanocytes.

  14. Noise in two-color electronic distance meter measurements revisited

    USGS Publications Warehouse

    Langbein, J.

    2004-01-01

    Frequent, high-precision geodetic data have temporally correlated errors. Temporal correlations directly affect both the estimate of rate and its standard error; the rate of deformation is a key product from geodetic measurements made in tectonically active areas. Various models of temporally correlated errors are developed and these provide relations between the power spectral density and the data covariance matrix. These relations are applied to two-color electronic distance meter (EDM) measurements made frequently in California over the past 15-20 years. Previous analysis indicated that these data have significant random walk error. Analysis using the noise models developed here indicates that the random walk model is valid for about 30% of the data. A second 30% of the data can be better modeled with power law noise with a spectral index between 1 and 2, while another 30% of the data can be modeled with a combination of band-pass-filtered plus random walk noise. The remaining 10% of the data can be best modeled as a combination of band-pass-filtered plus power law noise. This band-pass-filtered noise is a product of an annual cycle that leaks into adjacent frequency bands. For time spans of more than 1 year these more complex noise models indicate that the precision in rate estimates is better than that inferred by just the simpler, random walk model of noise.

  15. Streak camera based SLR receiver for two color atmospheric measurements

    NASA Technical Reports Server (NTRS)

    Varghese, Thomas K.; Clarke, Christopher; Oldham, Thomas; Selden, Michael

    1993-01-01

    To realize accurate two-color differential measurements, an image digitizing system with variable spatial resolution was designed, built, and integrated to a photon-counting picosecond streak camera, yielding a temporal scan resolution better than 300 femtosecond/pixel. The streak camera is configured to operate with 3 spatial channels; two of these support green (532 nm) and uv (355 nm) while the third accommodates reference pulses (764 nm) for real-time calibration. Critical parameters affecting differential timing accuracy such as pulse width and shape, number of received photons, streak camera/imaging system nonlinearities, dynamic range, and noise characteristics were investigated to optimize the system for accurate differential delay measurements. The streak camera output image consists of three image fields, each field is 1024 pixels along the time axis and 16 pixels across the spatial axis. Each of the image fields may be independently positioned across the spatial axis. Two of the image fields are used for the two wavelengths used in the experiment; the third window measures the temporal separation of a pair of diode laser pulses which verify the streak camera sweep speed for each data frame. The sum of the 16 pixel intensities across each of the 1024 temporal positions for the three data windows is used to extract the three waveforms. The waveform data is processed using an iterative three-point running average filter (10 to 30 iterations are used) to remove high-frequency structure. The pulse pair separations are determined using the half-max and centroid type analysis. Rigorous experimental verification has demonstrated that this simplified process provides the best measurement accuracy. To calibrate the receiver system sweep, two laser pulses with precisely known temporal separation are scanned along the full length of the sweep axis. The experimental measurements are then modeled using polynomial regression to obtain a best fit to the data. Data

  16. 30-micron heterodyne receiver

    NASA Technical Reports Server (NTRS)

    Kostiuk, Theodor; Spears, David L.

    1987-01-01

    Advantages and constraints of remote measurements using heterodyne spectroscopy near 30 microns are discussed. The state of the art of wideband HgCdTe photomixers and PbSnSe diode-laser local oscillators being developed for FIR heterodyne receivers is described. The first compact 30-micron heterodyne radiometer was built, and initial results at 28-microns show about 2-percent mixer efficiency for a 500-MHz-bandwidth receiver. Factors limiting receiver performance are discussed, along with the projected sensitivity of new interdigitated-electrode HgCdTe photoconductor mixers being developed for operation up to 200 microns.

  17. Directional deprotonation ionization of acetylene in asymmetric two-color laser fields

    NASA Astrophysics Data System (ADS)

    Song, Qiying; Gong, Xiaochun; Ji, Qinying; Lin, Kang; Pan, Haifeng; Ding, Jingxin; Zeng, Heping; Wu, Jian

    2015-05-01

    We experimentally investigate the deprotonation dissociative double ionization of an acetylene molecule by an asymmetric two-color laser pulse. We find that the ejection direction of the proton, and hence the directional C-H bond breaking of a polyatomic hydrocarbon molecule, can be controlled by finely tuning the phase of a two-color laser pulse.

  18. Ghost diffraction and ghost imaging in two-color ghost imaging

    NASA Astrophysics Data System (ADS)

    Yong, Pei; Fu, Xi-quan

    2016-10-01

    Ghost diffraction and ghost imaging in two-color ghost imaging are investigated with pseudo-thermal light. Based on the extended Huygens-Fresnel integral, the ghost diffraction (GD) and ghost imaging (GI) condition in two-color ghost imaging has been demonstrated. It is shown that GD and GI fringes in two-color ghost imaging can be obtained by conforming the GI condition and GD condition, respectively. The exchange of ghost diffraction and ghost imaging can be obtained by only changing one of the wavelength of two-color source when compared with the signal-color ghost imaging, and the condition of GD and GI in signal-color ghost imaging are a special case of two-color ghost imaging. The simulation results agree well with the theoretical analysis.

  19. Propagation characteristics of two-color laser pulses in homogeneous plasma

    SciTech Connect

    Hemlata,; Saroch, Akanksha; Jha, Pallavi

    2015-11-15

    An analytical and numerical study of the evolution of two-color, sinusoidal laser pulses in cold, underdense, and homogeneous plasma has been presented. The wave equations for the radiation fields driven by linear as well as nonlinear contributions due to the two-color laser pulses have been set up. A variational technique is used to obtain the simultaneous equations describing the evolution of the laser spot size, pulse length, and chirp parameter. Numerical methods are used to graphically analyze the simultaneous evolution of these parameters due to the combined effect of the two-color laser pulses. Further, the pulse parameters are compared with those obtained for a single laser pulse. Significant focusing, compression, and enhanced positive chirp is obtained due to the combined effect of simultaneously propagating two-color pulses as compared to a single pulse propagating in plasma.

  20. Correlated electron dynamics in nonsequential double ionization by orthogonal two-color laser pulses.

    PubMed

    Zhou, Yueming; Huang, Cheng; Tong, Aihong; Liao, Qing; Lu, Peixiang

    2011-01-31

    We have investigated the correlated electron dynamics in nonsequential double ionization (NSDI) of helium by the orthogonally polarized two-color pulses that consisted of an 800-nm and a 400-nm laser fields using the classical ensemble model. Depending on the relative phase of the two-color field, the electron momentum distributions along the polarization direction of the 800-nm field exhibit a surprisingly strong anticorrelated or correlated behavior. Back analysis reveals that recollisions eventually leading to NSDI are concentrated in a time window as short as several hundreds attoseconds with this scheme. By changing the relative phase of the two-color field, the revisit time of recolliding electron wave packet has been controlled with attosecond precision, which is responsible for the various correlated behaviors of the two electrons. Our results reveal that the orthogonally polarized two-color field can serve as a powerful tool to control the correlated electron dynamics in NSDI.

  1. On Two Color and CCD Methods for the Determination of Astronomic Position.

    DTIC Science & Technology

    1986-03-14

    u .tr ppow p.., w -1 " PRr~ PRO’w- r- VI . CURRENT PROBLEMS AND PROJECTED EQUIPMENT MODIFICATIONS This section will address the major problems which...RD-A174 088 ON TWO COLOR AND CCD METHODS FOR THE DETERMINATION Ot 1/2 ASTRONOMIC POSITION( U ) MARYLAND UNIV COLLEGE PARK DEPT OF PHYSICS AND ASTRONOMY...INTRODUCTION .................................... 2 A. Astroposition Objectives As Related to Two-Color Refractometry .................. 2 B. Results

  2. WFIRST Needs 3 Microns

    NASA Astrophysics Data System (ADS)

    Wright, Edward L.

    2012-05-01

    The Wide-Field InfraRed Survey Telescope (WFIRST) should work out to 4 microns instead of the current 2 or 2.5 micron cutoff for maximum science return. The signal-to-noise ratio on distant faint galaxies and quasars is maximized in the zodiacal light minimum at 3-4 microns. The SNR for measuring shapes for weak lensing also depends on PSF size relative to the galaxy size, but for an exponential scale length of 0.1" at z=2 (850 pc), the optimum wavelength is in the 2-3 micron range. While the PSF of a 1.3 m telescope at 3 microns has a FWHM of 0.5", this is still smaller than the seeing-limited PSF of LSST which plans to do weak lensing. The scatter in supernova Hubble diagrams is much lower for data taken in the rest frame near-infrared (0.11 mag) compared to rest-frame optical (0.18 mag), so at redshift z=2 one desires photometry at 3.75 microns. So each supernova observed in the rest-frame near-IR is worth 2-3 supernova observed in the rest frame optical. Microlensing is looking at zero redshift stars, and would use a filter to isolate an optimal bandpass, so it would be no worse with a longer detector cutoff wavelength. Slitless spectroscopy to measure Baryon Acoustic Oscillations will concentrate on redshifted H-alpha, and use a filter to select the desired redshift range, so a longer cutoff wavelength just gives more flexibility in targeting the redshift range. Thus the performance of WFIRST with a long cutoff wavelength is either substantially improved (for SNe and the high latitude survey), slightly improved (for weak lensing) or not hurt (for microlensing and BAO), and the overlap with the capabilities of Euclid is much reduced. NASA support for my participation on the WFIRST SDT is acknowledged.

  3. Localizing high-lying Rydberg wave packets with two-color laser fields

    NASA Astrophysics Data System (ADS)

    Larimian, Seyedreza; Lemell, Christoph; Stummer, Vinzenz; Geng, Ji-Wei; Roither, Stefan; Kartashov, Daniil; Zhang, Li; Wang, Mu-Xue; Gong, Qihuang; Peng, Liang-You; Yoshida, Shuhei; Burgdörfer, Joachim; Baltuška, Andrius; Kitzler, Markus; Xie, Xinhua

    2017-08-01

    We demonstrate control over the localization of high-lying Rydberg wave packets in argon atoms with phase-locked orthogonally polarized two-color laser fields. With a reaction microscope, we measure ionization signals of high-lying Rydberg states induced by a weak dc field and blackbody radiation as a function of the relative phase between the two-color fields. We find that the dc-field-ionization yield of high-lying Rydberg argon atoms oscillates with the relative two-color phase with a period of 2 π while the photoionization signal by blackbody radiation shows a period of π . Accompanying simulations show that these observations are a clear signature of the asymmetric localization of electrons recaptured into very elongated (low angular momentum) high-lying Rydberg states after conclusion of the laser pulse. Our findings thus open an effective pathway to control the localization of high-lying Rydberg wave packets.

  4. Magnetic Field Mental Representations of 14-15 Years Old Students

    ERIC Educational Resources Information Center

    Ravanis, Konstantinos; Pantidos, Panagiotis; Vitoratos, Evangelos

    2009-01-01

    Children's mental representations about physical concepts and phenomena play a vital role in the learning process. This is confirmed by the data derived from relevant researches which demonstrate that the students formulate incompatible ideas compared with the scientific ones. In this research we investigate the representations of 14-15 years old…

  5. Proceedings: National Conference on Bilingual Education (Austin, Texas, April 14-15, 1972).

    ERIC Educational Resources Information Center

    Dissemination and Assessment Center for Bilingual Education, Austin, TX.

    Goals of the National Conference on Bilingual Education, held on April 14-15, 1972 in Austin, Texas, were to emphasize bilingual education interaction at the national level using outstanding consultants from throughout the United States and to exchange ideas among educators in existing programs. The conference was also organized to give bilingual…

  6. Enhancement of terahertz radiation by using circularly polarized two-color laser fields

    NASA Astrophysics Data System (ADS)

    Meng, Chao; Chen, Wenbo; Wang, Xiaowei; Lü, Zhihui; Huang, Yindong; Liu, Jinlei; Zhang, Dongwen; Zhao, Zengxiu; Yuan, Jianmin

    2016-09-01

    Terahertz radiation from tunneling ionization of gaseous atoms and molecules in the two-color laser fields with various polarizations has been investigated. We experimentally demonstrate that the efficiency of terahertz emission in the circularly polarized laser fields with the same helicity is 5 times higher than that with linearly polarized two-color femtosecond pulses in high laser intensity. By solving time-dependent Schrödinger equation, this enhancement is well explained based on the analysis of electron tunneling ionization and subsequent dynamics.

  7. Two-color-laser-driven direct electron acceleration in infinite vacuum.

    PubMed

    Wong, Liang Jie; Kärtner, Franz X

    2011-03-15

    We propose a direct electron acceleration scheme that uses a two-color pulsed radially polarized laser beam. The two-color scheme achieves electron acceleration exceeding 90% of the theoretical energy gain limit, over twice of what is possible with a one-color pulsed beam of equal total energy and pulse duration. The scheme succeeds by exploiting the Gouy phase shift to cause an acceleration-favoring interference of fields only as the electron enters its effectively final accelerating cycle. Optimization conditions and power scaling characteristics are discussed.

  8. Generation of tunable and broadband far-infrared laser pulses during two-color filamentation

    SciTech Connect

    Theberge, Francis; Chateauneuf, Marc; Roy, Gilles; Mathieu, Pierre; Dubois, Jacques

    2010-03-15

    Tunable far-infrared laser pulses were generated efficiently during two-color filamentation in air. Understanding the creation of few-cycle far-infrared laser pulses is important since it is at the frontier between two possible generation mechanisms. The first one is the four-wave mixing generation, associated to the generation of wavelengths from ultraviolet up to mid-infrared laser pulses. The second process is the formation of transient photocurrent, which was recently used to describe the generation of submillimetric (terahertz) waves. Comparison between experiments and simulations revealed that the four-wave mixing mechanism is dominant for the far-infrared generation during two-color filamentation.

  9. Energy exchange between modes in a multimode two-color quantum dot laser with optical feedback.

    PubMed

    Virte, Martin; Pawlus, Robert; Sciamanna, Marc; Panajotov, Krassimir; Breuer, Stefan

    2016-07-15

    We investigate experimentally and theoretically the multimode dynamics of a two-color quantum dot laser subject to time-delayed optical feedback. We unveil energy exchanges between the longitudinal modes of the excited state triggered by variations of the feedback phase, and observe that the modal competition between longitudinal modes appears independently within the ground state and excited state emission. These features are accurately reproduced with a quantum dot laser model extended to take into account multiple modes for both ground and excited states. Finally, we discuss the significant impact of such behavior on feedback-based control of two-color quantum dot lasers.

  10. 14,15-EET promotes mitochondrial biogenesis and protects cortical neurons against oxygen/glucose deprivation-induced apoptosis

    SciTech Connect

    Wang, Lai; Chen, Man; Yuan, Lin; Xiang, Yuting; Zheng, Ruimao; Zhu, Shigong

    2014-07-18

    Highlights: • 14,15-EET inhibits OGD-induced apoptosis in cortical neurons. • Mitochondrial biogenesis of cortical neurons is promoted by 14,15-EET. • 14,15-EET preserves mitochondrial function of cortical neurons under OGD. • CREB mediates effect of 14,15-EET on mitochondrial biogenesis and function. - Abstract: 14,15-Epoxyeicosatrienoic acid (14,15-EET), a metabolite of arachidonic acid, is enriched in the brain cortex and exerts protective effect against neuronal apoptosis induced by ischemia/reperfusion. Although apoptosis has been well recognized to be closely associated with mitochondrial biogenesis and function, it is still unclear whether the neuroprotective effect of 14,15-EET is mediated by promotion of mitochondrial biogenesis and function in cortical neurons under the condition of oxygen–glucose deprivation (OGD). In this study, we found that 14,15-EET improved cell viability and inhibited apoptosis of cortical neurons. 14,15-EET significantly increased the mitochondrial mass and the ratio of mitochondrial DNA to nuclear DNA. Key makers of mitochondrial biogenesis, peroxisome proliferator activator receptor gamma-coactivator 1 alpha (PGC-1α), nuclear respiratory factor 1 (NRF-1) and mitochondrial transcription factor A (TFAM), were elevated at both mRNA and protein levels in the cortical neurons treated with 14,15-EET. Moreover, 14,15-EET markedly attenuated the decline of mitochondrial membrane potential, reduced ROS, while increased ATP synthesis. Knockdown of cAMP-response element binding protein (CREB) by siRNA blunted the up-regulation of PGC-1α and NRF-1 stimulated by 14,15-EET, and consequently abolished the neuroprotective effect of 14,15-EET. Our results indicate that 14,15-EET protects neurons from OGD-induced apoptosis by promoting mitochondrial biogenesis and function through CREB mediated activation of PGC-1α and NRF-1.

  11. Effects of a static electric field on two-color photoassociation between different atoms

    SciTech Connect

    Chakraborty, Debashree; Deb, Bimalendu

    2014-01-15

    We study non-perturbative effects of a static electric field on two-color photoassociation of different atoms. A static electric field induces anisotropy in scattering between two different atoms and hybridizes field-free rotational states of heteronuclear dimers or polar molecules. In a previous paper [D. Chakraborty et al., J. Phys. B 44, 095201 (2011)], the effects of a static electric field on one-color photoassociation between different atoms has been described through field-modified ground-state scattering states, neglecting electric field effects on heteronuclear diatomic bound states. To study the effects of a static electric field on heteronuclear bound states, and the resulting influence on Raman-type two-color photoassociation between different atoms in the presence of a static electric field, we develop a non-perturbative numerical method to calculate static electric field-dressed heteronuclear bound states. We show that the static electric field induced scattering anisotropy as well as hybridization of rotational states strongly influence two-color photoassociation spectra, leading to significant enhancement in PA rate and large shift. In particular, for static electric field strengths of a few hundred kV/cm, two-color PA rate involving high-lying bound states in electronic ground-state increases by several orders of magnitude even in the weak photoassociative coupling regime.

  12. Assessing probe-specific dye and slide biases in two-color microarray data

    USDA-ARS?s Scientific Manuscript database

    A primary reason for using two-color microarrays is that the use of two samples labeled with different dyes on the same slide and that bind to probes on the same spot is supposed to adjust for many factors that introduce noise and errors into the analysis. Most users assume that any differences bet...

  13. Observation of ionization enhancement in two-color circularly polarized laser fields

    NASA Astrophysics Data System (ADS)

    Mancuso, Christopher A.; Dorney, Kevin M.; Hickstein, Daniel D.; Chaloupka, Jan L.; Tong, Xiao-Min; Ellis, Jennifer L.; Kapteyn, Henry C.; Murnane, Margaret M.

    2017-08-01

    When atoms are irradiated by two-color circularly polarized laser fields the resulting strong-field processes are dramatically different than when the same atoms are irradiated by a single-color ultrafast laser. For example, electrons can be driven in complex two-dimensional trajectories before rescattering or circularly polarized high harmonics can be generated, which was once thought impossible. Here, we show that two-color circularly polarized lasers also enable control over the ionization process itself and make a surprising finding: the ionization rate can be enhanced by up to 700 % simply by switching the relative helicity of the two-color circularly polarized laser field. This enhancement is experimentally observed in helium, argon, and krypton over a wide range of intensity ratios of the two-color field. We use a combination of advanced quantum and fully classical calculations to explain this ionization enhancement as resulting in part due to the increased density of excited states available for resonance-enhanced ionization in counter-rotating fields compared with co-rotating fields. In the future, this effect could be used to probe the excited state manifold of complex molecules.

  14. 2011 Agile (Scrum) Workshop Held in Baltimore, Maryland on November 14-15, 2011

    DTIC Science & Technology

    2011-11-15

    Untitled Document 2011agile.html[3/23/2016 10:24:57 AM] Files are in Adobe format. Download the newest version from Adobe. 2011 AGILE ( SCRUM ...2750 HYATT REGENCY BALTIMORE u BALTIMORE, MD EVENT #2750 AGILE ( SCRUM ) WORKSHOP Sponsored by the C4ISR Division of NDIA AGILE ( SCRUM ) WORKSHOP...practices in DoD. AGILE ( SCRUM ) WORKSHOP NOVEMBER 14-15, 2011 GREG SHARP Software Development Team Lead, USAF PEX Mr. Greg Sharp is a Soft- ware

  15. Controlling the Motion of Electronic Wavepackets Using Cycle-Sculpted Two-Color Laser Fields

    NASA Astrophysics Data System (ADS)

    Kitzler, M.; Xie, X.; Roither, S.; Kartashov, D.; Baltuška, A.

    We use cycle-sculpted two-color waveforms to drive electronic wavepackets generated by strong-field ionization from helium, neon, and argon gas atoms and analyze their momentum spectra measured by electron-ion coincidence momentum spectroscopy. Varying the relative phase of the two colors allows to sculpt the ionizing field and hence to control the emission times and motion of the wavepackets on an attosecond timescale. Using semiclassical calculations, we investigate the influence of the ionic Coulomb field onto the motion of emitted electronic wavepackets. We further show that the measured electron momentum spectra contain interference patterns created by pairs of electron wavepackets that are released within a single laser-field cycle. We experimentally distinguish these subcycle interference structures from above-threshold ionization (ATI) peaks and argue that they can be used to extract the subcycle phase evolution of the laser-driven complex bound-state wavefunction.

  16. Two-color Laser Desorption of Nanostructured MgO Thin Films

    SciTech Connect

    Beck, Kenneth M.; Joly, Alan G.; Hess, Wayne P.

    2009-09-30

    Neutral magnesium atom emission from nanostructured MgO thin films is induced using two-color nanosecond laser excitation. We find that combined visible/UV excitation, for single-color pulse energies below the desorption threshold, induces neutral Mg-atom emission with hyperthermal kinetic energies in the range of 0.1- 0.2 eV. The observed metal atom emission is consistent with a mechanism involving rapid electron transfer to 3-coordinated Mg surface sites. The two-color Mg-atom signal is significant only for parallel laser polarizations and temporally overlapped laser pulses indicating that intermediate excited states are short-lived compared to the 5 nanosecond laser pulse duration.

  17. Two-color mid-infrared thermometer with a hollow glass optical fiber.

    PubMed

    Small, W; Celliers, P M; Da Silva, L B; Matthews, D L; Soltz, B A

    1998-10-01

    We have developed a low-temperature optical-fiber-based two-color infrared thermometer. A single 700-mum-bore hollow glass optical fiber collects and transmits radiation that is then modulated and split into two paths by a reflective optical chopper. Two different thermoelectrically cooled mid-infrared HgCdZnTe photoconductors monitor the chopped signals that are recovered with lock-in amplification. With the two previously obtained blackbody calibration equations, a computer algorithm calculates the true temperature and emissivity of a target in real time, taking into account reflection of the ambient radiation field from the target surface. The small numerical aperture of the hollow glass fiber and the fast response of the detectors, together with the two-color principle, permit high spatial and temporal resolution while allowing the user to dynamically alter the fiber-to-target distance.

  18. Recollision induced excitation-ionization with counter-rotating two-color circularly polarized laser field

    NASA Astrophysics Data System (ADS)

    Ben, Shuai; Guo, Pei-Ying; Pan, Xue-Fei; Xu, Tong-Tong; Song, Kai-Li; Liu, Xue-Shen

    2017-07-01

    Nonsequential double ionization of Ar by a counter-rotating two-color circularly polarized laser field is theoretically investigated. At the combined intensity in the ;knee; structure range, the double ionization occurs mainly through recollision induced excitation followed by subsequent ionization of Ar+∗ . By tracing the history of the recollision trajectories, we explain how the relative intensity ratio of the two colors controls the correlated electron dynamics and optimizes the ionization yields. The major channels contributing to enhancing the double ionization are through the elliptical trajectories with smaller travel time but not through the triangle shape or the other long cycle trajectories. Furthermore, the correlated electron dynamics could be limited to the attosecond time scale by adjusting the relative intensity ratio. Finally, the double ionization from doubly excited complex at low laser intensity is qualitatively discussed.

  19. Strong field ionization tomography with two-color circularly polarized femtosecond laser fields

    NASA Astrophysics Data System (ADS)

    Gopalakrishnan, Maithreyi; Mancuso, Chris; Hickstein, Daniel; Grychtol, Patrik; Knut, Ronny; Dollar, Franklin; Zusin, Dmitriy; Gentry, Christian; Turgut, Emrah; Ellis, Jennifer; Kapteyn, Henry; Murnane, Margaret; Kfir, Ofer; Cohen, Oren; Fleischer, Avner; Tong, Xiao-Min; Chen, Ming-Chang

    2015-03-01

    Recent experiments using two-color circularly polarized laser fields have demonstrated that high-harmonic generation (HHG), a versatile tabletop source of extreme ultraviolet (EUV) light, can be now extended to from linear to circular polarization. Here we present the first experiments using these uniquely polarized light fields to study strong field ionization (SFI), which is the complementary process to HHG. Using a velocity map imaging photoelectron spectroscopy and tomographic reconstruction techniques, we identify low-energy structures in the 3D photoelectron angular distributions that correspond to the rescattering of electrons with the ion. The observation of rescattering structures confirms the proposed explanation for HHG under two-color fields and paves the way for next-generation spectroscopies to investigate molecular structure.

  20. Making ultracold molecules in a two-color pump-dump photoassociation scheme using chirped pulses

    SciTech Connect

    Koch, Christiane P.; Luc-Koenig, Eliane; Masnou-Seeuws, Francoise

    2006-03-15

    This theoretical paper investigates the formation of ground state molecules from ultracold cesium atoms in a two-color scheme. Following previous work on photoassociation with chirped picosecond pulses [Luc-Koenig et al., Phys. Rev. A, 70, 033414 (2004)], we investigate stabilization by a second (dump) pulse. By appropriately choosing the dump pulse parameters and time delay with respect to the photoassociation pulse, we show that a large number of deeply bound molecules are created in the ground triplet state. We discuss (i) broad-bandwidth dump pulses which maximize the probability to form molecules while creating a broad vibrational distribution as well as (ii) narrow-bandwidth pulses populating a single vibrational ground state level, bound by 113 cm{sup -1}. The use of chirped pulses makes the two-color scheme robust, simple, and efficient.

  1. Two-color mid-infrared thermometer with a hollow glass optical fiber

    SciTech Connect

    Small, W. IV; Celliers, P.M.; Da Silva, L.B.; Matthews, D.L.; Soltz, B.A.

    1998-10-01

    We have developed a low-temperature optical-fiber-based two-color infrared thermometer. A single 700-{mu}m-bore hollow glass optical fiber collects and transmits radiation that is then modulated and split into two paths by a reflective optical chopper. Two different thermoelectrically cooled mid-infrared HgCdZnTe photoconductors monitor the chopped signals that are recovered with lock-in amplification. With the two previously obtained blackbody calibration equations, a computer algorithm calculates the true temperature and emissivity of a target in real time, taking into account reflection of the ambient radiation field from the target surface. The small numerical aperture of the hollow glass fiber and the fast response of the detectors, together with the two-color principle, permit high spatial and temporal resolution while allowing the user to dynamically alter the fiber-to-target distance. {copyright} 1998 Optical Society of America

  2. Airborne Astronomy with a 150 microns - 400 microns Heterodyne Spectrometer

    NASA Technical Reports Server (NTRS)

    Betz, A. L.

    1995-01-01

    This report summarizes work done under NASA Grant NAG2-753 awarded to the University of Colorado. The project goal was to build a far-infrared heterodyne spectrometer for NASA's Kuiper Airborne Observatory, and to use this instrument to observe atomic and molecular spectral lines from the interstellar medium. This goal was successfully achieved. Detections of particular note have been the 370 micron line of neutral atomic carbon, the 158 micron transition of ionized carbon, many of the high-J rotational lines of CO-12 and CO-13 between J=9-8 and J=22-21, the 119 micron and 163 micron rotational lines of OH, the 219 micron ground-state rotational line of H2D(+), and the 63 micron fine structure line of neutral atomic oxygen. All of these lines were observed at spectral resolutions exceeding 1 part in 10(exp 6) thereby allowing accurate line shapes and Doppler velocities to be measured.

  3. Airborne Astronomy with a 150 micron - 400 micron Heterodyne Spectrometer

    NASA Technical Reports Server (NTRS)

    Betz, A. L.

    1995-01-01

    This report summarizes work done under NASA Grant NAG2-753 awarded to the University of Colorado. The project goal was to build a far-infrared heterodyne spectrometer for NASA's Kuiper Airborne Observatory, and to use this instrument to observe atomic and molecular spectral lines from the interstellar medium. This goal was successfully achieved. Detections of particular note have been the 370 micron line of neutral atomic carbon, the 158 micron transition of ionized carbon, many of the high-J rotational lines of CO-12 and CO-13 between J=9-8 and J=22-21, the 119 micron and 163 micron rotational lines of OH, the 219 micron ground-state rotational line of H2D(+), and the 63 microns fine structure line of neutral atomic oxygen. All of these lines were observed at spectral resolutions exceeding 1 part in 10(exp 6), thereby allowing accurate line shapes and Doppler velocities to be measured.

  4. Potential for Terahertz/Optical, Two Color Non-linear Sensing of Liquid Biochemical Agents

    DTIC Science & Technology

    2011-05-18

    solutions1,2 in the infrared frequency band. To explore the lowest frequency macromo- lecular modes of biomolecules, which occur at terahertz fre- quencies, in...resonant with elec- tronic excitations and macromolecular vibrations . A configuration that optimizes SDFG in the face of strong terahertz absorption by...REPORT Potential for terahertz /optical, two color non-linear sensing of liquid biochemical agents 14. ABSTRACT 16. SECURITY CLASSIFICATION OF: A high

  5. A two-colored chewing gum test for assessing masticatory performance: a preliminary study.

    PubMed

    Endo, Toshiya; Komatsuzaki, Akira; Kurokawa, Hiroomi; Tanaka, Satoshi; Kobayashi, Yoshiki; Kojima, Koji

    2014-01-01

    This study was conducted to compare subjective and objective assessment methods of a two-colored chewing gum test and to find out whether these methods are capable of discriminating masticatory performances between sexes. 31 adults, 16 males and 15 females participated in this study. Each subject chewed five samples of two-colored chewing gum sticks for 5, 10, 20, 30 and 50 chewing strokes, respectively. The subjective color-mixing and shape indices for the gum bolus (SCMI-B, SSI-B) and the subjective color-mixing index and objective color-mixing ratio for the gum wafer (SCMI-W, OCMR-W) were evaluated by two independent examiners and, on a different day, re-evaluated by one of the examiners. The SCMI-B and SCMI-W assessments had inter- and intra-examiner reliable agreement at 20 or more chewing strokes. The OCMR-W measurement demonstrated high accuracy and low reproducibility between and within the examiners. There were significant gender differences in the distribution of SCMI-W scores (P = 0.044) and in the mean OCMI-W (P = 0.007). The SCMI-B and SCMI-W assessments and the OCMR-W measurement were reliable and valid at the 20 and 30 chewing strokes in this two-colored chewing gum test. The subjective color-mixing index (SCMI-W) and objective color-mixing ratio (OCMR-W) for the chewing gum wafer are capable of discriminating masticatory performance between sexes in this two-colored chewing gum test and that the OCMR-W measurement is discriminating better than the SCMI-W assessment.

  6. Two-color mixing for classifying agricultural products for safety and quality

    NASA Astrophysics Data System (ADS)

    Ding, Fujian; Chen, Yud-Ren; Chao, Kuanglin; Chan, Diane E.

    2006-02-01

    We show that the chromaticness of the visual signal that results from the two-color mixing achieved through an optically enhanced binocular device is directly related to the band ratio of light intensity at the two selected wavebands. A technique that implements the band-ratio criterion in a visual device by using two-color mixing is presented here. The device will allow inspectors to identify targets visually in accordance with a two-wavelength band ratio. It is a method of inspection by human vision assisted by an optical device, which offers greater flexibility and better cost savings than a multispectral machine vision system that implements the band-ratio criterion. With proper selection of the two narrow wavebands, discrimination by chromaticness that is directly related to the band ratio can work well. An example application of this technique for the inspection of carcasses chickens of afficted with various diseases is given. An optimal pair of wavelengths of 454 and 578 nm was selected to optimize differences in saturation and hue in CIE LUV color space among different types of target. Another example application, for the detection of chilling injury in cucumbers, is given, here the selected wavelength pair was 504 and 652 nm. The novel two-color mixing technique for visual inspection can be included in visual devices for various applications, ranging from target detection to food safety inspection.

  7. Two-Color STED Microscopy of Living Synapses Using A Single Laser-Beam Pair

    PubMed Central

    Tønnesen, Jan; Nadrigny, Fabien; Willig, Katrin I.; Wedlich-Söldner, Roland; Nägerl, U. Valentin

    2011-01-01

    The advent of superresolution microscopy has opened up new research opportunities into dynamic processes at the nanoscale inside living biological specimens. This is particularly true for synapses, which are very small, highly dynamic, and embedded in brain tissue. Stimulated emission depletion (STED) microscopy, a recently developed laser-scanning technique, has been shown to be well suited for imaging living synapses in brain slices using yellow fluorescent protein as a single label. However, it would be highly desirable to be able to image presynaptic boutons and postsynaptic spines, which together form synapses, using two different fluorophores. As STED microscopy uses separate laser beams for fluorescence excitation and quenching, incorporation of multicolor imaging for STED is more difficult than for conventional light microscopy. Although two-color schemes exist for STED microscopy, these approaches have several drawbacks due to their complexity, cost, and incompatibility with common labeling strategies and fluorophores. Therefore, we set out to develop a straightforward method for two-color STED microscopy that permits the use of popular green-yellow fluorescent labels such as green fluorescent protein, yellow fluorescent protein, Alexa Fluor 488, and calcein green. Our new (to our knowledge) method is based on a single-excitation/STED laser-beam pair to simultaneously excite and quench pairs of these fluorophores, whose signals can be separated by spectral detection and linear unmixing. We illustrate the potential of this approach by two-color superresolution time-lapse imaging of axonal boutons and dendritic spines in living organotypic brain slices. PMID:22098754

  8. Development of a new two color far infrared laser interferometer for future fusion devices

    SciTech Connect

    Kawahata, K.; Tanaka, K.; Tokuzawa, T.; Akiyama, T.; Ito, Y.; Okajima, S.; Nakayama, K.; Wylde, R.J.

    2004-10-01

    A new two color far infrared (FIR) laser interferometer under development for future fusion devices will be presented. The laser wavelength is optimized from the consideration of the beam refraction effect due to plasma density gradient and signal-to-noise ratio for an expected phase shift due to plasmas. Laser lines of 57.2 and 47.6 {mu}m are found to be suitable for the applications to high performance plasmas of Large Helical Device and future fusion devices such as the International Thermonuclear Experimental Reactor. The output power of 57.2 {mu}m CH{sub 3}OD laser is estimated to be {approx}1.6 W, which is the highest laser power in the FIR wavelength regime. The optical configuration of a new interferometer system using two colors will be proposed. In the system, one detector simultaneously detects the beat signals of the 57.2 and 47.6 {mu}m laser lines, and each interference signal can be separated electronically (1 MHz for 57.2 {mu}m and 0.84 MHz expected for 47.6 {mu}m). Mechanical vibration can be compensated by using the two color interferometer. The present status of the development of the system is also presented.

  9. Micronized grinding apparatus

    SciTech Connect

    Williams, R.M.

    1985-06-11

    Apparatus for grinding coal to micron fineness having a grinding chamber with a grinding surface supported by a circumferential wall in the grinding chamber, a plurality of grinding rolls orbiting in the grinding chamber for grinding the coal, air supply bustle surrounding the grinding chamber, air flow restrictor means opening from the air supply bustle to the grinding chamber to create a back pressure in the air supply bustle for substantially evenly distributing the air supplied to the grinding chamber around the circumference of the grinding chamber, and wherein the restrictor means directs the air flow tangentially relative to the circumferential wall of the grinding chamber so that the coal particles are caught up in a cyclonic movement having a large initial horizontally directed force to maintain a body of coal particles in the orbit of the grinding rolls, which horizontal force gradually diminishes as the vertical force component of the air flow lifts the ground coal particles out of the grinding chamber.

  10. Sub-micron filter

    DOEpatents

    Tepper, Frederick [Sanford, FL; Kaledin, Leonid [Port Orange, FL

    2009-10-13

    Aluminum hydroxide fibers approximately 2 nanometers in diameter and with surface areas ranging from 200 to 650 m.sup.2/g have been found to be highly electropositive. When dispersed in water they are able to attach to and retain electronegative particles. When combined into a composite filter with other fibers or particles they can filter bacteria and nano size particulates such as viruses and colloidal particles at high flux through the filter. Such filters can be used for purification and sterilization of water, biological, medical and pharmaceutical fluids, and as a collector/concentrator for detection and assay of microbes and viruses. The alumina fibers are also capable of filtering sub-micron inorganic and metallic particles to produce ultra pure water. The fibers are suitable as a substrate for growth of cells. Macromolecules such as proteins may be separated from each other based on their electronegative charges.

  11. Volcanic gas measurements at Mount Cleveland, 14-15 August 2015

    USGS Publications Warehouse

    Werner, Cynthia A.; Kern, Christoph; Kelly, Peter

    2017-01-01

    On 14-15 August 2015, helicopter-based measurements were made of the volcanic gases emitted from Mount Cleveland, AK. An upward-looking differential optical absorption spectroscopy (DOAS) system was used to measure incident scattered solar ultraviolet radiation while traversing beneath the plume on multiple occasions 14-15 August. This data was used to derive SO2 emission rates. Additionally, a Multi-Component Gas Analyzer System (Multi-GAS) was used to make measurements of trace gas concentrations while on a dedicated measurement flight passing through the gas plume on 15 August (19:15 - 19:56 UTC). Radiance spectra and gas compositions were both recorded at 1 second time resolution. Each spectrum and gas measurement was stamped with the GPS time and location. Each spectrum was saved in a separate ASCII file which includes 2048 radiances measured in the 285 - 430 nm spectral region and metadata associated with each acquisition. The Multi-GAS measurements are saved in a spreadsheet in the *.csv format.

  12. 14,15-EET promotes mitochondrial biogenesis and protects cortical neurons against oxygen/glucose deprivation-induced apoptosis.

    PubMed

    Wang, Lai; Chen, Man; Yuan, Lin; Xiang, Yuting; Zheng, Ruimao; Zhu, Shigong

    2014-07-18

    14,15-Epoxyeicosatrienoic acid (14,15-EET), a metabolite of arachidonic acid, is enriched in the brain cortex and exerts protective effect against neuronal apoptosis induced by ischemia/reperfusion. Although apoptosis has been well recognized to be closely associated with mitochondrial biogenesis and function, it is still unclear whether the neuroprotective effect of 14,15-EET is mediated by promotion of mitochondrial biogenesis and function in cortical neurons under the condition of oxygen-glucose deprivation (OGD). In this study, we found that 14,15-EET improved cell viability and inhibited apoptosis of cortical neurons. 14,15-EET significantly increased the mitochondrial mass and the ratio of mitochondrial DNA to nuclear DNA. Key makers of mitochondrial biogenesis, peroxisome proliferator activator receptor gamma-coactivator 1 alpha (PGC-1α), nuclear respiratory factor 1 (NRF-1) and mitochondrial transcription factor A (TFAM), were elevated at both mRNA and protein levels in the cortical neurons treated with 14,15-EET. Moreover, 14,15-EET markedly attenuated the decline of mitochondrial membrane potential, reduced ROS, while increased ATP synthesis. Knockdown of cAMP-response element binding protein (CREB) by siRNA blunted the up-regulation of PGC-1α and NRF-1 stimulated by 14,15-EET, and consequently abolished the neuroprotective effect of 14,15-EET. Our results indicate that 14,15-EET protects neurons from OGD-induced apoptosis by promoting mitochondrial biogenesis and function through CREB mediated activation of PGC-1α and NRF-1.

  13. Two-color resonant four-wave mixing: a tool for double resonance spectroscopy

    NASA Astrophysics Data System (ADS)

    Rohlfing, Eric A.; Tobiason, Joseph D.; Dunlop, J. R.; Williams, Skip

    1995-09-01

    Two-color resonant four-wave mixing (RFWM) shows great promise in a variety of double- resonance applications in molecular spectroscopy and chemical dynamics. One such application is stimulated emission pumping (SEP), which is a powerful method of characterizing ground-state potential energy surfaces in regions of chemical interest. We use time-independent, diagrammatic perturbation theory to identify the resonant terms in the third- order nonlinear susceptibility for each possible scheme by which two-color RFWM can be used for double-resonance spectroscopy. After a spherical tensor analysis we arrive at a signal expression for two-color RFWM that separates the molecular properties from purely laboratory-frame factors. In addition, the spectral response for tuning the DUMP laser in RFWM-SEP is found to be a simple Lorentzian in free-jet experiments. We demonstrate the utility of RFWM-SEP and test our theoretical predictions in experiments on jet-cooled transient molecules. In experiments on C3 we compare the two possible RFWM-SEP processes and show that one is particularly well-suited to the common situation in which the PUMP transition is strong but the DUMP transitions are weak. We obtain RFWM-SEP spectra of the formyl radical, HCO, that probe quasibound vibrational resonances lying above the low threshold for dissociation to H+CO. Varying the polarization of the input beams or PUMP rotational branch produces dramatic effects in the relative intensities of rotational lines in the RFWM-SEP spectra of HCO; these effects are well described by our theoretical analysis. Finally, RFWM-SEP spectra of HCO resonances that are homogeneously broadened by dissociation confirm the predicted lineshape and give widths that are in good agreement with those determined via unsaturated fluorescence depletion SEP.

  14. Assessing probe-specific dye and slide biases in two-color microarray data.

    PubMed

    Lu, Ruixiao; Lee, Geun-Cheol; Shultz, Michael; Dardick, Chris; Jung, Kihong; Phetsom, Jirapa; Jia, Yi; Rice, Robert H; Goldberg, Zelanna; Schnable, Patrick S; Ronald, Pamela; Rocke, David M

    2008-07-19

    A primary reason for using two-color microarrays is that the use of two samples labeled with different dyes on the same slide, that bind to probes on the same spot, is supposed to adjust for many factors that introduce noise and errors into the analysis. Most users assume that any differences between the dyes can be adjusted out by standard methods of normalization, so that measures such as log ratios on the same slide are reliable measures of comparative expression. However, even after the normalization, there are still probe specific dye and slide variation among the data. We define a method to quantify the amount of the dye-by-probe and slide-by-probe interaction. This serves as a diagnostic, both visual and numeric, of the existence of probe-specific dye bias. We show how this improved the performance of two-color array analysis for arrays for genomic analysis of biological samples ranging from rice to human tissue. We develop a procedure for quantifying the extent of probe-specific dye and slide bias in two-color microarrays. The primary output is a graphical diagnostic of the extent of the bias which called ECDF (Empirical Cumulative Distribution Function), though numerical results are also obtained. We show that the dye and slide biases were high for human and rice genomic arrays in two gene expression facilities, even after the standard intensity-based normalization, and describe how this diagnostic allowed the problems causing the probe-specific bias to be addressed, and resulted in important improvements in performance. The R package LMGene which contains the method described in this paper has been available to download from Bioconductor.

  15. Assessing probe-specific dye and slide biases in two-color microarray data

    PubMed Central

    Lu, Ruixiao; Lee, Geun-Cheol; Shultz, Michael; Dardick, Chris; Jung, Kihong; Phetsom, Jirapa; Jia, Yi; Rice, Robert H; Goldberg, Zelanna; Schnable, Patrick S; Ronald, Pamela; Rocke, David M

    2008-01-01

    Background A primary reason for using two-color microarrays is that the use of two samples labeled with different dyes on the same slide, that bind to probes on the same spot, is supposed to adjust for many factors that introduce noise and errors into the analysis. Most users assume that any differences between the dyes can be adjusted out by standard methods of normalization, so that measures such as log ratios on the same slide are reliable measures of comparative expression. However, even after the normalization, there are still probe specific dye and slide variation among the data. We define a method to quantify the amount of the dye-by-probe and slide-by-probe interaction. This serves as a diagnostic, both visual and numeric, of the existence of probe-specific dye bias. We show how this improved the performance of two-color array analysis for arrays for genomic analysis of biological samples ranging from rice to human tissue. Results We develop a procedure for quantifying the extent of probe-specific dye and slide bias in two-color microarrays. The primary output is a graphical diagnostic of the extent of the bias which called ECDF (Empirical Cumulative Distribution Function), though numerical results are also obtained. Conclusion We show that the dye and slide biases were high for human and rice genomic arrays in two gene expression facilities, even after the standard intensity-based normalization, and describe how this diagnostic allowed the problems causing the probe-specific bias to be addressed, and resulted in important improvements in performance. The R package LMGene which contains the method described in this paper has been available to download from Bioconductor. PMID:18638416

  16. Long wavelength mid-infrared from mixing two colors from a fiber amplifier

    NASA Astrophysics Data System (ADS)

    Bian, Siyuan; Loranger, Sébastien; Kashyap, Raman; Strickland, Donna

    2015-05-01

    At Waterloo, we are developing a high power, short pulse, two-color, Yb:fiber amplifier system to generate the long wavelength (<15μm) side of the molecular fingerprint spectral region, by difference frequency mixing the two colors. This spectral region is important for trace gas detection of explosives. As an example, it has been shown that the strong spectroscopic signatures of a peroxide-based explosive triacetone triperoxide (TATP) occur between 15 and 20 μm. To date, we have achieved a tuning range from 16 to 20 μm with a maximum average power of 1.7 mW. On the short wavelength side, the two colors would need to be pulled further apart, which requires a higher power seed to beat the amplified spontaneous emission that appears at the gain peak of the amplifiers between the two seed colors. On the long wavelength side, we are limited to 20 μm by the transparency region of the nonlinear crystals. We would like to find new nonlinear materials that have transparency from 1 to 30μm. If we could generate wavelengths from 15 to 30 μm with sufficient power, we could extend the spectral region to also cover 8 to 15μm by frequency doubling the longer wavelengths. We are currently working on replacing bulk optics in the system with fiber based optical elements to select the wavelengths as well as stretch and recompress the pulses in order to make the system compact and stable.

  17. A surface plasmonic coupled mid-long-infrared two-color quantum cascade detector

    NASA Astrophysics Data System (ADS)

    Li, Liang; Xiong, Dayuan; Wen, Jie; Li, Ning; Zhu, Ziqiang

    2016-11-01

    A novel mid-long-infrared two-color photodetector is proposed. It combines quantum cascade detector (QCD) and surface plasmonic coupling structure. The reflection spectrum and electric field are analyzed by algorithm of finite difference time domain method (FDTD). This QCD is sensitive to 4.4 μm and 9.0 μm infrared light. Mid-infrared and long-infrared pixels are interlaced arranged with specific plasmonic micro-cavity structures integrated. 7.1 and 7 times enhancement in optical absorption are obtained for mid-infrared and long-infrared pixels, respectively. Besides, a polarization-discriminating detection performance has been observed.

  18. Two-color phase-of-the-phase spectroscopy in the multiphoton regime

    NASA Astrophysics Data System (ADS)

    Almajid, M. A.; Zabel, M.; Skruszewicz, S.; Tiggesbäumker, J.; Bauer, D.

    2017-10-01

    Momentum-resolved photoelectron emission from xenon in colinearly polarized two-color laser fields at above-threshold ionization conditions is studied both experimentally and theoretically. We utilize phase-of-the-phase spectroscopy as recently introduced by Skruszewicz et al (2015 Phys. Rev. Lett. 115 043001) to analyze the dependence of the yields on the relative phase φ between the fundamental and second harmonic laser fields. The resulting phase-of-phase spectra feature a characteristic checkerboard pattern, which can analytically be described within the strong-field approximation.

  19. Fragment angular distribution in one- and two-color photodissociation by strong laser fields

    SciTech Connect

    Charron, E.; Giusti-Suzor, A.; Mies, F.H. Laboratoire de Chimie Physique, 11 rue Pierre et Marie Curie, 75231 Paris National Institute of Standards and Technology, Gaithersburg, Maryland 20899 )

    1994-02-01

    We present calculations for H[sub 2][sup +] photodissociation in intense short laser pulses where molecular rotation is fully included, resulting in the experimentally observed alignment of the photofragments. In addition, we show that by using a coherent superposition (phase-locked) of a fundamental radiation and its second harmonic, [ital a] [ital strong] [ital asymmetry] between the forwards and backwards proton distribution can be observed. Both the total dissociation probability and the asymmetry of the ion distribution are sensitive to the relative phase of the two colors and thus subject to coherent control.

  20. Two-color CO2/HeNe laser interferometer for C-2 experiment.

    PubMed

    Gornostaeva, O; Deng, B H; Garate, E; Gota, H; Kinley, J; Schroeder, J; Tuszewski, M

    2010-10-01

    A six-channel two-color interferometer has been developed for plasma electron density measurements in the C-2 field reversed configuration experiment. A CO(2) laser is utilized as the main probe beams, while copropagating visible HeNe laser beams are mainly sensitive to vibration. Density measurements in C-2 plasmas have shown that this is a reliable turn-key system. The maximum residual phase noise after vibration compensation is less than ±5°, corresponding to a line integral density of 3×10(18) m(-2). The time resolution for routine operation is 2 μs.

  1. Pressure-induced two-color photoluminescence in MnF(2) at room temperature.

    PubMed

    Hernández, I; Rodríguez, Fernando; Hochheimer, H D

    2007-07-13

    A novel two-color photoluminescence (PL) is found in MnF(2) at room temperature under high pressure. Contrary to low-temperature PL, PL at room temperature is unusual in transition-metal concentrated materials like MnF(2), since the deexcitation process at room temperature is fully governed by energy transfer to nonradiative centers. We show that room-temperature PL in MnF(2) originates from two distinct Mn(2+) emissions in the high-pressure cotunnite phase. The electronic structure and the excited-state dynamics are investigated by time-resolved emission and excitation spectroscopy at high pressure.

  2. Two-Color Single Hybrid Plasmonic Nanoemitters with Real Time Switchable Dominant Emission Wavelength.

    PubMed

    Zhou, Xuan; Wenger, Jérémie; Viscomi, Francesco N; Le Cunff, Loïc; Béal, Jérémie; Kochtcheev, Serguei; Yang, Xuyong; Wiederrecht, Gary P; Colas des Francs, Gérard; Bisht, Anu Singh; Jradi, Safi; Caputo, Roberto; Demir, Hilmi Volkan; Schaller, Richard D; Plain, Jérôme; Vial, Alexandre; Sun, Xiao Wei; Bachelot, Renaud

    2015-11-11

    We demonstrate two-color nanoemitters that enable the selection of the dominant emitting wavelength by varying the polarization of excitation light. The nanoemitters were fabricated via surface plasmon-triggered two-photon polymerization. By using two polymerizable solutions with different quantum dots, emitters of different colors can be positioned selectively in different orientations in the close vicinity of the metal nanoparticles. The dominant emission wavelength of the metal/polymer anisotropic hybrid nanoemitter thus can be selected by altering the incident polarization.

  3. Broadband two-color laser-induced incandescence pyrometry approach for nanoparticle characterization with improved sensitivity.

    PubMed

    Flügel, Alexandre; Kiefer, Johannes; Will, Stefan; Leipertz, Alfred

    2013-09-01

    A spectral filtering approach for improving the sensitivity of two-color laser-induced incandescence measurements is proposed. The commonly used bandpass filters providing wavelength selection, and hence temperature sensitivity, are replaced by shortpass and longpass filters, respectively, allowing significantly higher signal intensities to be detected. This modification is of particular interest when nanoparticles with low emissivity, for instance, metal and metal oxide particles, are investigated. An example case in which the conventional optical components are compared with the new approach reveals an improvement by more than one order of magnitude.

  4. Stokes image reconstruction for two-color microgrid polarization imaging systems.

    PubMed

    Lemaster, Daniel A

    2011-07-18

    The Air Force Research Laboratory has developed a new microgrid polarization imaging system capable of simultaneously reconstructing linear Stokes parameter images in two colors on a single focal plane array. In this paper, an effective method for extracting Stokes images is presented for this type of camera system. It is also shown that correlations between the color bands can be exploited to significantly increase overall spatial resolution. Test data is used to show the advantages of this approach over bilinear interpolation. The bounds (in terms of available reconstruction bandwidth) on image resolution are also provided.

  5. Simulation study of wakefield generation by two color laser pulses propagating in homogeneous plasma

    SciTech Connect

    Kumar Mishra, Rohit; Saroch, Akanksha; Jha, Pallavi

    2013-09-15

    This paper deals with a two-dimensional simulation of electric wakefields generated by two color laser pulses propagating in homogeneous plasma, using VORPAL simulation code. The laser pulses are assumed to have a frequency difference equal to the plasma frequency. Simulation studies are performed for two similarly as well as oppositely polarized laser pulses and the respective amplitudes of the generated longitudinal wakefields for the two cases are compared. Enhancement of wake amplitude for the latter case is reported. This simulation study validates the analytical results presented by Jha et al.[Phys. Plasmas 20, 053102 (2013)].

  6. Chiral random matrix theory for two-color QCD at high density

    SciTech Connect

    Kanazawa, Takuya; Yamamoto, Naoki; Wettig, Tilo

    2010-04-15

    We identify a non-Hermitian chiral random matrix theory that corresponds to two-color QCD at high density. We show that the partition function of the random matrix theory coincides with the partition function of the finite-volume effective theory at high density, and that the Leutwyler-Smilga-type spectral sum rules of the random matrix theory are identical to those derived from the effective theory. The microscopic Dirac spectrum of the theory is governed by the BCS gap, rather than the conventional chiral condensate. We also show that with a different choice of a parameter the random matrix theory yields the effective partition function at low density.

  7. Nondestructive inspection of explosive materials using linearly polarized two-colored photon beam

    NASA Astrophysics Data System (ADS)

    Toyokawa, H.; Hayakawa, T.; Shizuma, T.; Hajima, R.; Masuda, K.; Ohgaki, H.

    2011-10-01

    A nondestructive inspection method for screening explosive materials that are hidden in passenger vehicles, trucks, and cargo containers with radiation shielding was presented. The method was examined experimentally using linearly polarized two-colored photon beam. A sample object was irradiated with the photon beam, followed by an emission of gamma-rays in nuclear resonance fluorescence. The gamma-rays from oxygen and nitrogen emitted through nuclear resonance fluorescence were measured using high-purity germanium detectors. We were able to evaluate the element concentration ratio.

  8. Critical line from imaginary to real baryonic chemical potentials in two-color QCD

    SciTech Connect

    Cea, Paolo; Cosmai, Leonardo; D'Elia, Massimo; Papa, Alessandro

    2008-03-01

    The method of analytic continuation from imaginary to real chemical potentials {mu} is one of the few available techniques to study QCD at finite temperature and baryon density. One of its most appealing applications is the determination of the critical line for small {mu}: we perform a direct test of the validity of the method in this case by studying two-color QCD, where the sign problem is absent. The (pseudo)critical line is found to be analytic around {mu}{sup 2}=0, but a very large precision would be needed at imaginary {mu} to correctly predict the location of the critical line at real {mu}.

  9. Controlling Nonsequential Double Ionization in Two-Color Circularly Polarized Femtosecond Laser Fields

    NASA Astrophysics Data System (ADS)

    Mancuso, Christopher A.; Dorney, Kevin M.; Hickstein, Daniel D.; Chaloupka, Jan L.; Ellis, Jennifer L.; Dollar, Franklin J.; Knut, Ronny; Grychtol, Patrik; Zusin, Dmitriy; Gentry, Christian; Gopalakrishnan, Maithreyi; Kapteyn, Henry C.; Murnane, Margaret M.

    2016-09-01

    Atoms undergoing strong-field ionization in two-color circularly polarized femtosecond laser fields exhibit unique two-dimensional photoelectron trajectories and can emit bright circularly polarized extreme ultraviolet and soft-x-ray beams. In this Letter, we present the first experimental observation of nonsequential double ionization in these tailored laser fields. Moreover, we can enhance or suppress nonsequential double ionization by changing the intensity ratio and helicity of the two driving laser fields to maximize or minimize high-energy electron-ion rescattering. Our experimental results are explained through classical simulations, which also provide insight into how to optimize the generation of circularly polarized high harmonic beams.

  10. Confocal imaging at the nanoscale with two-color STED microscopy

    NASA Astrophysics Data System (ADS)

    Gugel, Hilmar; Giske, Arnold; Dyba, Marcus; Sieber, Jochen

    2011-03-01

    STED microscopy enables confocal imaging of biological samples with a resolution that is not limited by diffraction. It provides new insights in various fields of biology, such as membrane biology, neurobiology and physiology. Its three dimensional sectioning ability allows the acquisition of high resolution image stacks. Furthermore, STED microscopy enables the recording of dynamic processes and live cell images. We present two-color imaging in confocal STED microscopy with a single STED wavelength. Pulsed and continuous wave lasers in the visible and near infra-red wavelengths range are used for stimulated emission. The resolution enhancement is demonstrated in comparison to confocal imaging with biological specimens.

  11. Palmitoylethanolamide: problems regarding micronization, ultra-micronization and additives.

    PubMed

    Kriek, Rutger

    2014-06-01

    It can be established that at least two of the writers of the article published in 'Inflammopharmacology', title: 'Palmitoylethanolamide (PEA), a naturally occurring disease-modifying agent in neuropathic pain' have a direct connection to the companies Epitech and Innovet. These companies produce micronized and ultra-micronized PEA. Therefore it is of eminent importance to determine whether the statements in this paper have also taken into consideration the European guidelines for Good Clinical Practice and the codes of good scientific practices. This is very questionable. A minimum condition in clinical studies for proving the claim that PEA in its micronized and ultra-micronized formulations works better than in its pure form or in other formulations is that a comparison be made between: PEA in pure form or in other formulations, on the one hand; PEA in the micronized and ultra-micronized formulations, on the other hand. This minimum condition is not complied with. Based on additional studies discussed in this commentary and in view of the effects of ultra-micronization on the parameters discussed, as well as the potential side-effects of additives such as excipients and herbal extracts added to the products cited in the article, the preference should be for the time being to treat patients with pure PEA without any of these additives.

  12. Absolute distance measurement with correction of air refractive index by using two-color dispersive interferometry.

    PubMed

    Wu, Hanzhong; Zhang, Fumin; Liu, Tingyang; Li, Jianshuang; Qu, Xinghua

    2016-10-17

    Two-color interferometry is powerful for the correction of the air refractive index especially in the turbulent air over long distance, since the empirical equations could introduce considerable measurement uncertainty if the environmental parameters cannot be measured with sufficient precision. In this paper, we demonstrate a method for absolute distance measurement with high-accuracy correction of air refractive index using two-color dispersive interferometry. The distances corresponding to the two wavelengths can be measured via the spectrograms captured by a CCD camera pair in real time. In the long-term experiment of the correction of air refractive index, the experimental results show a standard deviation of 3.3 × 10-8 for 12-h continuous measurement without the precise knowledge of the environmental conditions, while the variation of the air refractive index is about 2 × 10-6. In the case of absolute distance measurement, the comparison with the fringe counting interferometer shows an agreement within 2.5 μm in 12 m range.

  13. Excitation dynamics involving homogeneous multistate interactions: one and two color VMI and REMPI of HBr.

    PubMed

    Hróðmarsson, Helgi Rafn; Kartakoullis, Andreas; Zaouris, Dimitris; Glodic, Pavle; Wang, Huasheng; Samartzis, Peter C; Kvaran, Ágúst

    2017-05-10

    Velocity map imaging (VMI) data and mass resolved REMPI spectra are complementarily utilized to elucidate the involvement of homogeneous multistate interactions in excited state dynamics of HBr. The H(1)Σ(+)(v' = 0) and E(1)Σ(+)(v' = 1) Rydberg states and the V(1)Σ(+)(v'= m + 7) and V(1)Σ(+)(v'= m + 8) ion-pair states are explored as a function of rotational quantum number in the two-photon excitation region of 79 100-80 700 cm(-1). H(+) and Br(+) images were recorded by one- as well as two-color excitation schemes. Kinetic energy release (KER) spectra and angular distributions were extracted from the data. Strong-to-medium interactions between the E(1) and V(m + 8)/V(m + 7) states on one hand and the H(0) and V(m + 7)/V(m + 8) states on the other hand were quantified from peak shifts and intensity analysis of REMPI spectra. The effects of those interactions on subsequent photoionization and photolytic pathways of HBr were evaluated in one-color VMI experiments of the H(+) and two-color VMI experiments of the Br(+) photoproducts.

  14. Cell autonomous defects in cortical development revealed by two-color chimera analysis.

    PubMed

    Kwiatkowski, Adam V; Garner, Craig C; Nelson, W James; Gertler, Frank B

    2009-05-01

    A complex program of cell intrinsic and extrinsic signals guide cortical development. Although genetic studies in mice have uncovered roles for numerous genes and gene families in multiple aspects of corticogenesis, determining their cell autonomous functions is often complicated by pleiotropic defects. Here we describe a novel lentiviral-based method to analyze cell autonomy by generating two-color chimeric mouse embryos. Ena/VASP-deficient mutant and control embryonic stem (ES) cells were labeled with different fluorescent chimeric proteins (EGFP and mCherry) that were modified to bind to the plasma membrane. These labeled ES cells were used to generate two-color chimeric embryos possessing two genetically distinct populations of cortical cells, permitting multiple aspects of neuronal morphogenesis to be analyzed and compared between the two cell populations. We observed little difference between the ability of control and Ena/VASP-deficient cells to contribute to cortical organization during development. In contrast, we observed axon fiber tracts originating from control neurons but not Ena/VASP-deficient neurons, indicating that loss of Ena/VASP causes a cell autonomous defect in cortical axon formation. This technique could be applied to determine other cell autonomous functions in different stages of cortical development.

  15. Two-Colored Dental Surveying Tool as an Alternative for Carbon Marker.

    PubMed

    Patil, Pravinkumar G; Nimbalkar-Patil, Smita

    2017-07-01

    Various tools are used with a dental surveyor, including analyzing rods, carbon markers, undercut gauges, and protective sheaths for a specific function. A carbon marker is a parallel-sided carbon rod used to mark the survey line on a cast or a crown on a cast. The carbon marker (with or without protective sheath) cannot differentiate more than one survey line on the cast if needed. The wear of the carbon marker along the parallel walls after repeated use may give an incorrect survey line. We suggest a simple modification in the analyzing rod to prepare a two-colored surveying tool. An analyzing rod is a parallel-sided rod used to analyze the relative parallelism of two or more surfaces of a cast and to mark survey lines on wax patterns. With the modified analyzing rod, the survey lines can be marked with two colors, and the problem of breaking of the carbon marker also can be eliminated. © 2015 by the American College of Prosthodontists.

  16. Two-color probe of high harmonic generation from argon atoms

    NASA Astrophysics Data System (ADS)

    Zhao, Zengxiu; Yuan, Jianmin; Meng, Chao; Chen, Wenbo

    2014-05-01

    Two-color control of high harmonic generation has been proven a powerful in situ tool to characterize the intrinsic chirp of attosecond bursts. The weak second harmonic pulse introduces a phase modulation of the strong field quantum processes, leading to the generation of even-order harmonic. We measure the yields of even-order harmonics from argon gases as a function of the phase delay between the fundamental and its second harmonic pulse. We found that the modulation of even-order harmonics exhibits a phase jump around the 28th harmonic (48eV), closely resembling the result from. However, we show by varying laser intensity that the phase jump is unlikely to be attributed to the switching from short to long trajectories of HHG near the cut-off. In addition, we demonstrate that the phase of jump depends on the driving laser wavelength. Single-active-electron simulation fails to reproduce the experimental observation. We therefore suspect that multielectron response comes into play for the two-color control of HHG from Argon. Preliminary analysis suggests that there exists competing pathways of HHG from inner orbitals, even for argon atoms whose interaction with strong laser fields is usually assumed well described by SAE approximation.

  17. Intensity-dependent two-electron emission dynamics with orthogonally polarized two-color laser fields

    NASA Astrophysics Data System (ADS)

    Yuan, Zongqiang; Ye, Difa; Xia, Qinzhi; Liu, Jie; Fu, Libin

    2015-06-01

    In this paper, we explore the intensity-dependent strong-field double ionization of Ne with orthogonally polarized two-color laser pulses consisting of 800- and 400-nm laser fields. The yield of Ne2+ as a function of the relative phase Δ ϕ of the two colors experiences a qualitative transition as the laser intensity decreases from the saturation regime to the far-below-saturation regime. In the saturation regime, our simulations well reproduce the recent experimental observations [Phys. Rev. Lett. 112, 193002 (2014), 10.1103/PhysRevLett.112.193002]. Turning to the far-below-saturation regime, however, we find that the observed small knee structure totally disappears and the maximum yield of Ne2+ is shifted by a π /2 phase. This is explained by the competition between the trajectory concentration effect and the Δ ϕ -dependent ionization rate of the tunneling electron. The possibility of controlling over the two-electron emission direction along the 400-nm field through the laser intensity is also investigated. We show that the two-electron emission direction can be reversed by changing the laser intensity for some vales of Δ ϕ , while this fails for some other values of Δ ϕ .

  18. Diquark and pion condensation in random matrix models for two-color QCD

    SciTech Connect

    Klein, B.; Toublan, D.; Verbaarschot, J.J.M.

    2005-07-01

    We introduce a random matrix model with the symmetries of QCD with two colors at nonzero isospin and baryon chemical potentials and temperature. We analyze its phase diagram and find phases with condensation of pion and diquark states in addition to the phases with spontaneously broken chiral symmetries. In the limit of small chemical potentials and quark masses, we reproduce the mean field results obtained from chiral Lagrangians. As in the case of QCD with three colors, the presence of two chemical potentials breaks the flavor symmetry and leads to phases that are characterized by different behaviors of the chiral condensates for each flavor. In particular, the phase diagram we obtain is similar to QCD with three colors and three flavors of quarks of equal masses at zero baryon chemical potential and nonzero isospin and strange chemical potentials. A tricritical point of the superfluid transitions found in lattice calculations and from an analysis in terms of chiral Lagrangians does not appear in the random matrix model. Remarkably, at fixed isospin chemical potential, for the regions outside of the superfluid phases, the phase diagrams in the temperature--baryon chemical potential plane for two colors and three colors are qualitatively the same.

  19. Controlling electron-ion rescattering in two-color circularly polarized femtosecond laser fields

    NASA Astrophysics Data System (ADS)

    Mancuso, Christopher A.; Hickstein, Daniel D.; Dorney, Kevin M.; Ellis, Jennifer L.; Hasović, Elvedin; Knut, Ronny; Grychtol, Patrik; Gentry, Christian; Gopalakrishnan, Maithreyi; Zusin, Dmitriy; Dollar, Franklin J.; Tong, Xiao-Min; Milošević, Dejan B.; Becker, Wilhelm; Kapteyn, Henry C.; Murnane, Margaret M.

    2016-05-01

    High-harmonic generation driven by two-color counter-rotating circularly polarized laser fields was recently demonstrated experimentally as a breakthrough source of bright, coherent, circularly polarized beams in the extreme ultraviolet and soft-x-ray regions. However, the conditions for optimizing the single-atom yield are significantly more complex than for linearly polarized driving lasers and are not fully understood. Here we present a comprehensive study of strong-field ionization—the complementary process to high-harmonic generation—driven by two-color circularly polarized fields. We uncover the conditions that lead to enhanced electron-ion rescattering, which should correspond to the highest single-atom harmonic flux. Using a velocity map imaging photoelectron spectrometer and tomographic reconstruction techniques, we record three-dimensional photoelectron distributions resulting from the strong-field ionization of argon atoms across a broad range of driving laser intensity ratios. In combination with analytical predictions and advanced numerical simulations, we show that "hard" electron-ion rescattering is optimized when the second-harmonic field has an intensity approximately four times higher than that of the fundamental driving field. We also investigate electron-ion rescattering with co-rotating fields, and find that rescattering is significantly suppressed when compared with counter-rotating fields.

  20. True temperature measurement on metallic surfaces using a two-color pyroreflectometer method.

    PubMed

    Hernandez, D; Netchaieff, A; Stein, A

    2009-09-01

    In the most common case of optical pyrometry, the major obstacle in determining the true temperature is the knowledge of the thermo-optical properties for in situ conditions. We present experimental results obtained with a method able to determine the true temperature of metallic surfaces above 500 degrees C when there is not parasitic effect by surrounding radiation. The method is called bicolor pyroreflectometry and it is based on Planck's law, Kirchhoff's law, and the assumption of identical reflectivity indicatrixes for the target surface at two different close wavelengths (here, 1.3 and 1.55 microm). The diffusion factor eta(d), the key parameter of the method, is introduced to determine the convergence temperature T(*), which is expected to be equal to the true temperature T. Our goal is to asses this method for different metallic surfaces. The validation of this method is made by comparison with thermocouples. Measurements were made for tungsten, copper, and aluminum samples of different roughnesses, determined by a rugosimeter. After introducing a theoretical model for two-color pyroreflectometry, we give a description of the experimental setup and present experimental applications of the subject method. The quality of the results demonstrates the usefulness of two-color pyroreflectometry to determine the temperatures of hot metals when the emissivity is not known and for the commercially important case of specular surfaces.

  1. An RNA-aptamer-based two-color CRISPR labeling system

    PubMed Central

    Wang, Siyuan; Su, Jun-Han; Zhang, Feng; Zhuang, Xiaowei

    2016-01-01

    The spatial organization and dynamics of chromatin play important roles in essential biological functions. However, direct visualization of endogenous genomic loci in living cells has proven to be laborious until the recent development of CRISPR-Cas9-based chromatin labeling methods. These methods rely on the recognition of specific DNA sequences by CRISPR single-guide RNAs (sgRNAs) and fluorescent–protein-fused catalytically inactive Cas9 to label specific chromatin loci in cells. Previously, multicolor chromatin labeling has been achieved using orthogonal Cas9 proteins from different bacterial species fused to different fluorescent proteins. Here we report the development of an alternative two-color CRISPR labeling method using only the well-characterized Streptococcus pyogenes Cas9, by incorporating MS2 or PP7 RNA aptamers into the sgRNA. The MS2 or PP7 aptamers then recruit the corresponding MS2 or PP7 coat proteins fused with different fluorescent proteins to the target genomic loci. Here we demonstrate specific and orthogonal two-color labeling of repetitive sequences in living human cells using this method. By attaching the MS2 or PP7 aptamers to different locations on the sgRNA, we found that extending the tetraloop and stem loop 2 of the sgRNA with MS2 or PP7 aptamers enhances the signal-to-background ratio of chromatin imaging. PMID:27229896

  2. Generation of strong terahertz field from two-color laser filamentation and optical rectification

    NASA Astrophysics Data System (ADS)

    Kuk, Donghoon; Yoo, Yungjun; Oh, Taek Il; You, Yong Sing; Kim, Ki-Yong

    2015-04-01

    We have demonstrated strong-field (>8 MV/cm), high-peak-power (12 MW) THz generation with a bandwidth of >20 THz via two-color laser filamentation. Moderate average power (1.4 mW) is also achieved by using a cryogenically-cooled Ti:sapphire amplifier capable of producing 30 fs, 15 mJ pulses at a 1 kHz repetition rate. For maximal THz generation and transmission, we have used a combination of a thin dichroic waveplate and a large Brewster-angled silicon filter. Here we have used a thin BBO crystal for frequency doubling (800 nm to 400 nm) and observed strong terahertz emission from the crystal itself. We also find that this type of terahertz emission can be optimized to yield more output power compared to two-color photoionization. In both cases, we have used a microbolometer camera for real-time THz beam profiling. This cost-effective THz camera along with our intense THz sources can be a useful tool for nonlinear THz studies including broadband THz spectroscopy and imaging. Work supported by DOE, Office of Basic Energy Sciences, Division of Materials Sciences and Engineering under Award No. 014216-001.

  3. THz generation by two-color femtosecond filaments with complex polarization states: four-wave mixing versus photocurrent contributions

    NASA Astrophysics Data System (ADS)

    Fedorov, V. Yu; Koulouklidis, A. D.; Tzortzakis, S.

    2017-01-01

    Two-color filamenation in gases is known to produce intense and broadband THz radiation. There are two physical mechanisms responsible for the THz generation in this scheme: four-wave mixing and emission from the induced plasma currents. The case when the main and second harmonic are linearly polarized is well studied including the impact from each of the above mechanisms. However, for the cases when the two-color fields have complex polarization states the role of the four-wave mixing and plasma mechanisms in the formation of the THz polarization is still under-explored. Here we use both the four-wave mixing and photocurrent models in order to consider the THz generation by two-color fields with arbitrary polarizations. We show that under specific polarizations of the two-color field components it is possible to determine which of the mechanisms is responsible for the THz polarization formation.

  4. Control of the yield of surviving Rydberg atoms in strong-field ionization with two-color laser fields

    NASA Astrophysics Data System (ADS)

    Ge, Peipei; Liu, Yunquan

    2017-06-01

    We theoretically investigate the controllable yield of neutral excited atoms surviving in the tunneling ionization process using two-color fields with parallel and orthogonal polarizations. Using the classical-trajectory Monte Carlo model, we show that the yield of the surviving Rydberg atoms after tunneling ionization can be modulated by varying the relative phase of the two frequency components. Tracing back to the initial tunneling coordinates at the tunnel exit of the electrons that contribute to the surviving atoms, we find the distribution of these coordinates shifts with the relative phase and the polarization configuration of the two-color fields. The orthogonally polarized two-color field counterintuitively tends to recapture more electrons than the two-color field with parallel polarizations.

  5. Two dye combinations suitable for two-color/two-dye laser-induced fluorescence thermography for ethanol

    NASA Astrophysics Data System (ADS)

    Fenner, Andreas; Stephan, Peter

    2017-06-01

    This paper presents two dye combinations suitable for two-color/two-dye laser-induced fluorescence thermography for ethanol. Besides the temperature dependency of the fluorescence, the influences of laser fluence, dye concentration, pressure, dissolved air, and photobleaching are also discussed. The experimental data are compared with models and data available in literature. Based on this, parameter ranges for two-color/two-dye laser-induced fluorescence thermography applications can be determined.

  6. Human platelets produce 14,15-oxido-5,8,11-eicosatrienoic acid from phosphatidylinositol

    SciTech Connect

    Ballou, L.R.; Lam, B.K.; Wong, P.Y.K.; Cheung, W.Y.

    1987-05-01

    Human platelets contain a soluble enzyme or enzyme system which catalyzes the formation of a compound more polar than arachidonate from 2-arachidonyl-sn-phosphatidylinositol (PtdIns). The C-value and mass spectrum of the compound appears similar to the reported values of 14,15-oxido-5,8,11-eicosatrienoic acid (EET). 2-Arachidonyl-sn-phosphatidylcholine, 2-arachidonyl-sn-phosphatidylethanolamine and arachidonic acid were not substrates for EET production. The reaction was Ca/sup 2 +/-dependent and insensitive to aspirin, mepacrin and indomethacin. EET formation was greatly reduced under nitrogen or carbon monoxide, however, exposure to atmospheric air rapidly restored EET production to a rate comparable to that under air. Further, neither NADPH nor cyanide affected EET formation, suggesting that a cytochrome P-450 system was not involved. Intact platelets prelabeled with (/sup 14/C)arachidonic acid generated at least 0.5 nmole of EET/10/sup 9/ platelets in response to thrombin; other agonists such as collagen, epinephrine, ADP or ionophore A23187 were not effective. Collectively, these data suggest that human platelets possess an enzyme system which appears to catalyze epoxidation of the arachidonyl moiety of PtdIns and its subsequent hydrolysis to yield EET.

  7. Comparative in vitro activities of new 14-, 15-, and 16-membered macrolides.

    PubMed Central

    Hardy, D J; Hensey, D M; Beyer, J M; Vojtko, C; McDonald, E J; Fernandes, P B

    1988-01-01

    The in vitro activities of several 14-, 15- and 16-membered macrolides were compared with that of erythromycin. In general, 14-membered macrolides such as erythromycin, clarithromycin, and flurithromycin were more active against streptococci and Bordetella pertussis than was the 15-membered macrolide azithromycin, which was more active than 16-membered macrolides such as miocamycin and rokitamycin. Clarithromycin was the most active compound against Streptococcus pyogenes, pneumococci, Listeria monocytogenes, and Corynebacterium species. Legionella pneumophila was most susceptible to miocamycin, clarithromycin, and rokitamycin. Branhamella catarrhalis, Neisseria gonorrhoeae, and Haemophilus influenzae were most susceptible to azithromycin. Azithromycin and dirithromycin were the most active compounds against Campylobacter jejuni. MICs of 16-membered macrolides for strains expressing inducible-type resistance to erythromycin were less than or equal to 1 microgram/ml, whereas none of the compounds had activity against strains expressing constitutive-type resistance. The MICs of roxithromycin, miocamycin, rokitamycin, and josamycin increased in the presence of human serum, whereas MICs of the other compounds either were unchanged or decreased. PMID:3252753

  8. Hominoid fission of chromosome 14/15 and the role of segmental duplications.

    PubMed

    Giannuzzi, Giuliana; Pazienza, Michele; Huddleston, John; Antonacci, Francesca; Malig, Maika; Vives, Laura; Eichler, Evan E; Ventura, Mario

    2013-11-01

    Ape chromosomes homologous to human chromosomes 14 and 15 were generated by a fission event of an ancestral submetacentric chromosome, where the two chromosomes were joined head-to-tail. The hominoid ancestral chromosome most closely resembles the macaque chromosome 7. In this work, we provide insights into the evolution of human chromosomes 14 and 15, performing a comparative study between macaque boundary region 14/15 and the orthologous human regions. We construct a 1.6-Mb contig of macaque BAC clones in the region orthologous to the ancestral hominoid fission site and use it to define the structural changes that occurred on human 14q pericentromeric and 15q subtelomeric regions. We characterize the novel euchromatin-heterochromatin transition region (∼20 Mb) acquired during the neocentromere establishment on chromosome 14, and find it was mainly derived through pericentromeric duplications from ancestral hominoid chromosomes homologous to human 2q14-qter and 10. Further, we show a relationship between evolutionary hotspots and low-copy repeat loci for chromosome 15, revealing a possible role of segmental duplications not only in mediating but also in "stitching" together rearrangement breakpoints.

  9. Low-Latitude Auroras: The Magnetic Storm of 14-15 May 1921

    NASA Technical Reports Server (NTRS)

    Silverman, S. M.; Cliver, E. W.

    2001-01-01

    We review solar geophysical data relating to the great magnetic storm of 14-15 May 1921, with emphasis on observations of the low-latitude visual aurora. From the reports we have gathered for this event the lowest geomagnetic latitude of definite overhead aurora (coronal form) was 40 deg and the lowest geomagnetic latitude from which auroras were observed on the poleward horizon in the northern hemisphere was 30 deg. For comparison, corresponding overhead/low-latitude values of 48 deg/32 deg and 41 deg/20 deg were reported for the great auroras on 28-29 August and 1-2 September 1859, respectively. However for the 1921 event, there is a report of aurora from Apia, Samoa, in the southern hemisphere, within 13 deg of the geomagnetic equator. This report by professional observers appears to be credible, based on the aurora description and timing, but is puzzling because of the discrepancy with the lowest latitude of observation in the northern hemisphere and the great implied aurora height (approximately 2000 km, assuming overhead aurora at Auckland, New Zealand). We discuss various possibilities that might account for this observation.

  10. Two-Color Coherent Control of Optical Bistability in Asymmetric Semiconductor Quantum Wells

    NASA Astrophysics Data System (ADS)

    Li, Jia-Hua; Hao, Xiang-Ying

    We investigate optical bistability in intersubband transitions of an asymmetric semiconductor quantum well structure that has equidistant transitions between three subbands of the system and is placed in a unidirectional cavity. The system is simultaneously coupled by a fundamental field and its second harmonic. The second harmonic field acts as a control field and significantly influences the optical bistability. In addition, the two-color coherent control of optical bistability by the relative phase of the fundamental and the second harmonic fields is shown. The influence of the electronic cooperation parameter on the OB behavior is also discussed. This investigation may be used for optimizing and controlling the optical switching process in the SQW solid-state system, which is much more practical than that in the atomic system because of its flexible design and the controllable interference strength.

  11. Synthesis of Two-Color Laser Pulses for the Harmonic Cutoff Extension

    NASA Astrophysics Data System (ADS)

    Wang, Guo-Li; Zhou, Li-Hua; Zhao, Song-Feng; Zhou, Xiao-Xin

    2016-05-01

    Increasing simultaneously both the cutoff energy and efficiency is a big challenge to all applications of high-order harmonic generation (HHG). For this purpose, the shaping of the waveform of driving pulse is an alternative approach. Here, we show that the harmonic cutoff can be extended by about two times without reducing harmonic yield after considering macroscopic propagation effects, by adopting a practical way to synthesize two-color fields with fixed energy. Our results, combined with the experimental techniques, show the great potential of HHG as a tabletop light source. Supported by the National Natural Science Foundation of China under Grant Nos. 11264036, 11164025, 11364038, the Specialized Research Fund for the Doctoral Program of Higher Education of China under Grant No. 20116203120001, and the Basic Scientific Research Foundation for Institution of Higher Learning of Gansu Province

  12. Statistical analysis of efficient unbalanced factorial designs for two-color microarray experiments.

    PubMed

    Tempelman, Robert J

    2008-01-01

    Experimental designs that efficiently embed a fixed effects treatment structure within a random effects design structure typically require a mixed-model approach to data analyses. Although mixed model software tailored for the analysis of two-color microarray data is increasingly available, much of this software is generally not capable of correctly analyzing the elaborate incomplete block designs that are being increasingly proposed and used for factorial treatment structures. That is, optimized designs are generally unbalanced as it pertains to various treatment comparisons, with different specifications of experimental variability often required for different treatment factors. This paper uses a publicly available microarray dataset, as based upon an efficient experimental design, to demonstrate a proper mixed model analysis of a typical unbalanced factorial design characterized by incomplete blocks and hierarchical levels of variability.

  13. Statistical Analysis of Efficient Unbalanced Factorial Designs for Two-Color Microarray Experiments

    PubMed Central

    Tempelman, Robert J.

    2008-01-01

    Experimental designs that efficiently embed a fixed effects treatment structure within a random effects design structure typically require a mixed-model approach to data analyses. Although mixed model software tailored for the analysis of two-color microarray data is increasingly available, much of this software is generally not capable of correctly analyzing the elaborate incomplete block designs that are being increasingly proposed and used for factorial treatment structures. That is, optimized designs are generally unbalanced as it pertains to various treatment comparisons, with different specifications of experimental variability often required for different treatment factors. This paper uses a publicly available microarray dataset, as based upon an efficient experimental design, to demonstrate a proper mixed model analysis of a typical unbalanced factorial design characterized by incomplete blocks and hierarchical levels of variability. PMID:18584033

  14. Quantitative surface temperature measurement using two-color thermographic phosphors and video equipment

    NASA Technical Reports Server (NTRS)

    Buck, Gregory M. (Inventor)

    1989-01-01

    A thermal imaging system provides quantitative temperature information and is particularly useful in hypersonic wind tunnel applications. An object to be measured is prepared by coating with a two-color, ultraviolet-activated, thermographic phosphor. The colors emitted by the phosphor are detected by a conventional color video camera. A phosphor emitting blue and green light with a ratio that varies depending on temperature is used so that the intensity of light in the blue and green wavelengths detected by the blue and green tubes in the video camera can be compared. Signals representing the intensity of blue and green light at points on the surface of a model in a hypersonic wind tunnel are used to calculate a ratio of blue to green light intensity which provides quantitative temperature information for the surface of the model.

  15. Calibration of a two-color soft x-ray diagnostic for electron temperature measurement

    NASA Astrophysics Data System (ADS)

    Reusch, L. M.; Den Hartog, D. J.; Franz, P.; Goetz, J.; McGarry, M. B.; Stephens, H. D.

    2016-11-01

    The two-color soft x-ray (SXR) tomography diagnostic on the Madison Symmetric Torus is capable of making electron temperature measurements via the double-filter technique; however, there has been a 15% systematic discrepancy between the SXR double-filter (SXRDF) temperature and Thomson scattering (TS) temperature. Here we discuss calibration of the Be filters used in the SXRDF measurement using empirical measurements of the transmission function versus energy at the BESSY II electron storage ring, electron microprobe analysis of filter contaminants, and measurement of the effective density. The calibration does not account for the TS and SXRDF discrepancy, and evidence from experiments indicates that this discrepancy is due to physics missing from the SXRDF analysis rather than instrumentation effects.

  16. Theoretical and experimental analyses of the performance of two-color laser ranging systems

    NASA Technical Reports Server (NTRS)

    Im, K. E.; Gardner, C. S.

    1985-01-01

    The statistical properties of the signals reflected from the retroreflector equipped satellites were studied. It is found that coherence interference between pulse reflections from retroreflectors of different ranges on the array platform is the primary cause of signal fluctuations. The performance of a cross-correlation technique to estimate the differential propagation time is analyzed by considering both shot noise and speckle. For the retroreflector arrays, timing performance is dominated by interference induced speckle, and the differential propagation time cannot be resolved to better than the pulse widths of the received signals. The differential timing measurements obtained over a horizontal path are analyzed. The ocean-reflected pulse measurements obtained from the airborne two-color laser altimeter experiment are presented.

  17. Two-color photoexcitation of Rydberg states via an electric quadrupole transition

    SciTech Connect

    Li Leping; Gu Quanli; Knee, J. L.; Wright, J. D.; DiSciacca, J. M.; Morgan, T. J.

    2008-03-15

    We report the observation of an electric quadrupole transition between the 4s{sup '}[1/2]{sub 0}{sup o} and 3d[3/2]{sub 2}{sup o} states in the spectrum of argon and use it in the first step of a scheme to excite Rydberg states. The initial identification of the transition is based on one-color, two-photon photoionization. A different experiment utilizing two-color, two-photon photoexcitation to Rydberg states confirms the identification. Despite the unavoidable background of one-color, two-photon photoionization, the latter experimental technique makes possible two-photon spectroscopy of Rydberg states using a resonant intermediate state populated by an electric quadrupole transition.

  18. Two-color corrugated quantum-well infrared photodetector for remote temperature sensing

    NASA Astrophysics Data System (ADS)

    Chen, C. J.; Choi, K. K.; Chang, W. H.; Tsui, D. C.

    1998-01-01

    A quantum-well infrared photodetector (QWIP) based on the corrugated light-coupling scheme has been fabricated and tested for remote temperature sensing. The QWIP consists of two stacks of multiple quantum wells (MQWs), each sensitive in one of the atmospheric infrared transmission windows and each with a separate readout circuit. High optical coupling efficiency is obtained in both wavelength ranges, demonstrating the use of the corrugated structure for two-color detection. By monitoring the ratio of the photocurrent generated simultaneously in each MQW stack, the temperature of the object emitting the radiation can be determined, regardless of its emissivity and the geometrical factors. This temperature sensing ability is tested by using a blackbody radiator with precision temperature control as the target. The agreement between the measured and the preset temperatures indicates that the corrugated QWIP is capable of precision thermometric measurements.

  19. Monitoring coherent electron wave packet excitation dynamics by two-color attosecond laser pulses

    NASA Astrophysics Data System (ADS)

    Yuan, Kai-Jun; Bandrauk, André D.

    2016-11-01

    We propose a method to monitor coherent electron wave packet (CEWP) excitation dynamics with two-color attosecond laser pulses. Simulations are performed on aligned H2+ by numerically solving the three-dimensional time-dependent Schrödinger equation with combinations of a resonant linearly polarized λl= 100/70 nm pump pulse and a circularly polarized λc=5 nm attosecond probe pulse. It is found that time dependent diffraction patterns in molecular frame photoelectron angular distributions (MFPADs) produced by the circular probe pulse exhibit sensitivity to the molecular alignments and time-dependent geometry of the CEWPs during and after the coherent excitation between the ground and excited states induced by the linear pump pulse. The time dependent MFPADs are described by an ultrafast diffraction model for the ionization of the bound CEWPs.

  20. Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila

    PubMed Central

    Gambis, Alexis; Dourlen, Pierre; Steller, Hermann; Mollereau, Bertrand

    2011-01-01

    We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The “Tomato/GFP-FLP/FRT” method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single cell resolution. PMID:21215264

  1. Fabrication of optical multilayer for two-color phase plate in super-resolution microscope.

    PubMed

    Iketaki, Yoshinori; Kitagawa, Katsuichi; Hidaka, Kohjiro; Kato, Naoki; Hirabayashi, Akira; Bokor, Nandor

    2014-07-01

    In super-resolution microscopy based on fluorescence depletion, the two-color phase plate (TPP) is an indispensable optical element, which can independently control the phase shifts for two beams of different color, i.e., the pump and erase beams. By controlling a phase shift of the erase beam through the TPP, the erase beam can be modulated into a doughnut shape, while the pump beam maintains the initial Gaussian shape. To obtain a reliable optical multiplayer (ML) for the TPP, we designed a ML with only two optical layers by performing numerical optimization. The measured phase shifts generated by the fabricated ML using interferometry correspond to the design values. The beam profiles in the focal plane are also consistent with theoretical results. Although the fabricated ML consists of only two optical layers, the ML can provide a suitable phase modulation function for the TPP in a practical super-resolution microscope.

  2. Approaching the full octave: noncollinear optical parametric chirped pulse amplification with two-color pumping.

    PubMed

    Herrmann, D; Homann, C; Tautz, R; Scharrer, M; Russell, P St J; Krausz, F; Veisz, L; Riedle, E

    2010-08-30

    We present a new method to broaden the amplification range in optical parametric amplification toward the bandwidth needed for single cycle femtosecond pulses. Two-color pumping of independent stages is used to sequentially amplify the long and short wavelength parts of the ultrabroadband seed pulses. The concept is tested in two related experiments. With multi-mJ pumping pulses with a nearly octave spanning spectrum and an uncompressed energy of 3 mJ are generated at low repetition rate. The spectral phase varies slowly and continuously in the overlap region as shown with 100 kHz repetition rate. This should allow the compression to the Fourier limit of below 5 fs in the high energy system.

  3. Terahertz emission driven by two-color laser pulses at various frequency ratios

    NASA Astrophysics Data System (ADS)

    Wang, W.-M.; Sheng, Z.-M.; Li, Y.-T.; Zhang, Y.; Zhang, J.

    2017-08-01

    We present a simulation study of terahertz radiation from a gas driven by two-color laser pulses in a broad range of frequency ratios ω1/ω0 . Our particle-in-cell simulation results show that there are three series with ω1/ω0=2 n , n +1 /2 , n ±1 /3 (n is a positive integer) for high-efficiency and stable radiation generation. The radiation strength basically decreases with the increasing ω1 and scales linearly with the laser wavelength. These rules are broken when ω1/ω0<1 and much stronger radiation may be generated at any ω1/ω0 . These results can be explained with a model based on gas ionization by two linear-superposition laser fields, rather than a multiwave mixing model.

  4. Fabrication of optical multilayer for two-color phase plate in super-resolution microscope

    SciTech Connect

    Iketaki, Yoshinori; Kitagawa, Katsuichi; Hidaka, Kohjiro; Kato, Naoki; Hirabayashi, Akira; Bokor, Nandor

    2014-07-15

    In super-resolution microscopy based on fluorescence depletion, the two-color phase plate (TPP) is an indispensable optical element, which can independently control the phase shifts for two beams of different color, i.e., the pump and erase beams. By controlling a phase shift of the erase beam through the TPP, the erase beam can be modulated into a doughnut shape, while the pump beam maintains the initial Gaussian shape. To obtain a reliable optical multiplayer (ML) for the TPP, we designed a ML with only two optical layers by performing numerical optimization. The measured phase shifts generated by the fabricated ML using interferometry correspond to the design values. The beam profiles in the focal plane are also consistent with theoretical results. Although the fabricated ML consists of only two optical layers, the ML can provide a suitable phase modulation function for the TPP in a practical super-resolution microscope.

  5. Nonadiabatic Electron Dynamics in Orthogonal Two-Color Laser Fields with Comparable Intensities.

    PubMed

    Geng, Ji-Wei; Xiong, Wei-Hao; Xiao, Xiang-Ru; Peng, Liang-You; Gong, Qihuang

    2015-11-06

    We theoretically investigate the nonadiabatic subcycle electron dynamics in orthogonally polarized two-color laser fields with comparable intensities. The photoelectron dynamics is simulated by exact solution to the 3D time-dependent Schrödinger equation, and also by two other semiclassical methods, i.e., the quantum trajectory Monte Carlo simulation and the Coulomb-corrected strong field approximation. Through these methods, we identify the underlying mechanisms of the subcycle electron dynamics and find that both the nonadiabatic effects and the Coulomb potential play very important roles. The contribution of the nonadiabatic effects manifest in two aspects, i.e., the nonadiabatic ionization rate and the nonzero initial velocities at the tunneling exit. The Coulomb potential has a different impact on the electrons' trajectories for different relative phases between the two pulses.

  6. Spatial properties of a terahertz beam generated from a two-color air plasma

    NASA Astrophysics Data System (ADS)

    Klarskov, Pernille; Wang, Tianwu; Buron, Jonas D.; Strikwerda, Andrew C.; Jepsen, Peter U.

    2013-09-01

    We present a spatial characterization of terahertz (THz) beams generated from a two-color air plasma under different conditions by measuring full 3D beam profiles using a commercial THz camera. We compare two THz beam profiles emitted from plasmas generated by 35 fs and 100 fs laser pulses, and show that the spatial properties of the two THz beams do not change significantly. For the THz beam profile generated by the 35 fs pulse, the spatial effect of eliminating the lower frequencies is investigated by implementing two crossed polarizers working as a high-pass filter. We show that this reduces the beam waist, and that the beam spot shape changes from Lorentzian to Gaussian. Finally, we observe a forward-propagating Gaussian THz beam by spatially filtering away the conical off-axis radiation with a 1 cm aperture.

  7. Two color satellite laser ranging upgrades at Goddard's 1.2m telescope facility

    NASA Technical Reports Server (NTRS)

    Zagwodzki, Thomas W.; Mcgarry, Jan F.; Degnan, John J.; Varghese, Thomas K.

    1993-01-01

    The ranging laboratory at Goddard's 1.2 m telescope tracking facility has recently been upgraded to include a single photoelectron sensitive Hamamatsu streak camera-based range receiver which uses doubled and tripled Nd:YAG frequencies for satellite laser ranging. Other ranging system upgrades include a new continuum laser, which will deliver up to 30 millijoules (mJ) at both 532 and 355 nm at a pulsewidth of 30 picoseconds (FWHM), and replacement of both ranging and tracking computers with COMPAQ 386 based systems. Preliminary results using a photomultiplier-tube based receiver and waveform digitizer indicate agreement within the accuracy of the measurement with the theoretical Marini and Murray model for atmospheric refraction. Two color streak camera measurements are used to further analyze the accuracy of these and other atmospheric refraction models.

  8. Two-color continuous-variable quantum entanglement in a singly resonant optical parametric oscillator

    SciTech Connect

    Cuozzo, Domenico; Oppo, Gian-Luca

    2011-10-15

    We apply the input-output theory of optical cavities to formulate a quantum treatment of a continuous-wave singly resonant optical parametric oscillator. This case is mainly relevant to highly nondegenerate signal and idler modes. We show that both intensity and quadrature squeezing are present and that the maximum noise reduction below the standard quantum limit is the same at the signal and idler frequencies as in the doubly resonant case. As the threshold of oscillation is approached, however, the intensity-difference and quadrature spectra display a progressive line narrowing which is absent in the balanced doubly resonant case. By use of the separability criterion for continuous variables, the signal-idler state is found to be entangled over wide ranges of the parameters. We show that attainable levels of squeezing and entanglement make singly resonant configurations ideal candidates for two-color quantum information processes, because of their ease of tuning in experimental realizations.

  9. Two-color corrugated quantum-well infrared photodetector for remote temperature sensing

    SciTech Connect

    Chen, C.J.; Choi, K.K.; Chang, W.H.; Tsui, D.C.

    1998-01-01

    A quantum-well infrared photodetector (QWIP) based on the corrugated light-coupling scheme has been fabricated and tested for remote temperature sensing. The QWIP consists of two stacks of multiple quantum wells (MQWs), each sensitive in one of the atmospheric infrared transmission windows and each with a separate readout circuit. High optical coupling efficiency is obtained in both wavelength ranges, demonstrating the use of the corrugated structure for two-color detection. By monitoring the ratio of the photocurrent generated simultaneously in each MQW stack, the temperature of the object emitting the radiation can be determined, regardless of its emissivity and the geometrical factors. This temperature sensing ability is tested by using a blackbody radiator with precision temperature control as the target. The agreement between the measured and the preset temperatures indicates that the corrugated QWIP is capable of precision thermometric measurements. {copyright} {ital 1998 American Institute of Physics.}

  10. Dissociative double ionization of CO in orthogonal two-color laser fields

    NASA Astrophysics Data System (ADS)

    Song, Qiying; Lu, Peifen; Gong, Xiaochun; Ji, Qinying; Lin, Kang; Zhang, Wenbin; Ma, Junyang; Zeng, Heping; Wu, Jian

    2017-01-01

    We experimentally investigate dissociative double ionization of CO by a phase-controlled orthogonal two-color (OTC) laser pulse. Directional breaking of doubly ionized CO as a function of both kinetic energy and emission direction of the nuclear fragments is observed in the polarization plane steered by the laser phase. It is attributed to the dominating sequential double ionization at the maximum strength and nonsequential double ionization at a relatively weak strength of the spatiotemporally shaped oscillating laser field pointing to various directions. Our results are interesting not only for two-dimensional control of directional bond breaking, but also strengthen our understanding of strong-field sequential and nonsequential double ionization of molecules which are spatiotemporally streaked to various directions and kinetic energies by an OTC laser pulse.

  11. Frequency doubler and two-color mode of operation at free electron laser FLASH2

    NASA Astrophysics Data System (ADS)

    Kuhlmann, M.; Schneidmiller, E. A.; Yurkov, M. V.

    2017-06-01

    We report on the results of the first operation of a frequency doubler at FLASH2. The scheme uses the feature of the variable gap of the undulator. Undulator is divided in two parts. The second part of the undulator is tuned to the double frequency of the first part. Modulated electron beam enters the second part of the undulator and generates radiation at the 2nd harmonic. Depending on a balance between the gain of undulator sections, frequency doubler allows operation in a two-color mode and operation at shorter wavelengths with respect to standard SASE scheme. The shortest wavelength of 3.1 nm (photon energy 400 eV) has been achieved at FLASH2 with frequency doubler scheme, which is significantly below the design value for the standard SASE option.

  12. Spectral Linewidth Narrowing and Tunable Two-Color Laser Operation of Two Diode Laser Arrays

    SciTech Connect

    Liu, Bo; Braiman, Yehuda

    2012-01-01

    We propose and implement a common external cavity to narrow spectral linewidth of two broad-area laser diode arrays (LDAs) and align their center wavelengths. The locked center wavelength of two LDAs can be tuned in the range of {approx}10 nm by tuning the tilted angle of the diffraction grating. The output beams of two LDAs are spatially overlapped through the polarization beam splitter of the common external cavity, and the total output power equals the power of two LDAs. The center wavelength of each LDA can be independently tuned by shifting the corresponding fast-axis collimation lens. As a result, the high-power two-color LDA operation is demonstrated with the tunable wavelength difference of up to 2 nm ({approx}1 THz).

  13. Enhanced hole boring with two-color relativistic laser pulses in the fast ignition scheme

    SciTech Connect

    Yu, Changhai; Tian, Ye; Li, Wentao; Wang, Wentao; Zhang, Zhijun; Qi, Rong; Wang, Cheng; Deng, Aihua; Liu, Jiansheng

    2016-08-15

    A scheme of using two-color laser pulses for hole boring into overdense plasma as well as energy transfer into electron and ion beams has been studied using particle-in-cell simulations. Following an ultra-short ultra-intense hole-boring laser pulse with a short central wavelength in extreme ultra-violet range, the main infrared driving laser pulse can be guided in the hollow channel preformed by the former laser and propagate much deeper into an overdense plasma, as compared to the case using the infrared laser only. In addition to efficiently transferring the main driving laser energy into energetic electrons and ions generation deep inside the overdense plasma, the ion beam divergence can be greatly reduced. The results might be beneficial for the fast ignition concept of inertial confinement fusion.

  14. Generation of scalable terahertz radiation from cylindrically focused two-color laser pulses in air

    SciTech Connect

    Kuk, D.; Yoo, Y. J.; Rosenthal, E. W.; Jhajj, N.; Milchberg, H. M.; Kim, K. Y.

    2016-03-21

    We demonstrate scalable terahertz (THz) generation by focusing terawatt, two-color laser pulses in air with a cylindrical lens. This focusing geometry creates a two-dimensional air plasma sheet, which yields two diverging THz lobe profiles in the far field. This setup can avoid plasma-induced laser defocusing and subsequent THz saturation, previously observed with spherical lens focusing of high-power laser pulses. By expanding the plasma source into a two-dimensional sheet, cylindrical focusing can lead to scalable THz generation. This scheme provides an energy conversion efficiency of 7 × 10{sup −4}, ∼7 times better than spherical lens focusing. The diverging THz lobes are refocused with a combination of cylindrical and parabolic mirrors to produce strong THz fields (>21 MV/cm) at the focal point.

  15. Calibration of a two-color soft x-ray diagnostic for electron temperature measurement

    SciTech Connect

    Reusch, L. M. Den Hartog, D. J.; Goetz, J.; McGarry, M. B.; Franz, P.; Stephens, H. D.

    2016-11-15

    The two-color soft x-ray (SXR) tomography diagnostic on the Madison Symmetric Torus is capable of making electron temperature measurements via the double-filter technique; however, there has been a 15% systematic discrepancy between the SXR double-filter (SXR{sub DF}) temperature and Thomson scattering (TS) temperature. Here we discuss calibration of the Be filters used in the SXR{sub DF} measurement using empirical measurements of the transmission function versus energy at the BESSY II electron storage ring, electron microprobe analysis of filter contaminants, and measurement of the effective density. The calibration does not account for the TS and SXR{sub DF} discrepancy, and evidence from experiments indicates that this discrepancy is due to physics missing from the SXR{sub DF} analysis rather than instrumentation effects.

  16. Attosecond x-ray source generation from two-color polarized gating plasmonic field enhancement

    SciTech Connect

    Feng, Liqiang; Yuan, Minghu; Chu, Tianshu

    2013-12-15

    The plasmonic field enhancement from the vicinity of metallic nanostructures as well as the polarization gating technique has been utilized to the generation of the high order harmonic and the single attosecond x-ray source. Through numerical solution of the time-dependent Schrödinger equation, for moderate the inhomogeneity and the polarized angle of the two fields, we find that not only the harmonic plateau has been extended and enhanced but also the single short quantum path has been selected to contribute to the harmonic. As a result, a series of 50 as pulses around the extreme ultraviolet and the x-ray regions have been obtained. Furthermore, by investigating the other parameters effects on the harmonic emission, we find that this two-color polarized gating plasmonic field enhancement scheme can also be achieved by the multi-cycle pulses, which is much better for experimental realization.

  17. Two-color monochromatic x-ray imaging with a single short-pulse laser

    NASA Astrophysics Data System (ADS)

    Sawada, H.; Daykin, T.; McLean, H. S.; Chen, H.; Patel, P. K.; Ping, Y.; Pérez, F.

    2017-06-01

    Simultaneous monochromatic crystal imaging at 4.5 and 8.0 keV with x-rays produced by a single short-pulse laser is presented. A layered target consisting of thin foils of titanium and copper glued together is irradiated by the 50 TW Leopard short-pulse laser housed at the Nevada Terawatt Facility. Laser-accelerated MeV fast electrons transmitting through the target induce Kα fluorescence from both foils. Two energy-selective curved crystals in the imaging diagnostic form separate monochromatic images on a single imaging detector. The experiment demonstrates simultaneous two-color monochromatic imaging of the foils on a single detector as well as Kα x-ray production at two different photon energies with a single laser beam. Application of the diagnostic technique to x-ray radiography of a high density plasma is also presented.

  18. Mechanisms of two-color laser-induced field-free molecular orientation.

    PubMed

    Spanner, Michael; Patchkovskii, Serguei; Frumker, Eugene; Corkum, Paul

    2012-09-14

    Two mechanisms of two-color (ω+2ω) laser-induced field-free molecular orientation, based on the hyperpolarizability and ionization depletion, are explored and compared. The CO molecule is used as a computational example. While the hyperpolarizability mechanism generates small amounts of orientation at intensities below the ionization threshold, ionization depletion quickly becomes the dominant mechanism as soon as ionizing intensities are reached. Only the ionization mechanism leads to substantial orientation (e.g., on the order of ≳0.1). For intensities typical of laser-induced molecular alignment and orientation experiments, the two mechanisms lead to robust, characteristic timings of the field-free orientation wave-packet revivals relative to the alignment revivals and the revival time. The revival timings can be used to detect the active orientation mechanism experimentally.

  19. Energy-Resolved Ultrashort Delays of Photoelectron Emission Clocked by Orthogonal Two-Color Laser Fields.

    PubMed

    Gong, Xiaochun; Lin, Cheng; He, Feng; Song, Qiying; Lin, Kang; Ji, Qinying; Zhang, Wenbin; Ma, Junyang; Lu, Peifen; Liu, Yunquan; Zeng, Heping; Yang, Weifeng; Wu, Jian

    2017-04-07

    A phase-controlled orthogonal two-color (OTC) femtosecond laser pulse is employed to probe the time delay of photoelectron emission in the strong-field ionization of atoms. The OTC field spatiotemporally steers the emission dynamics of the photoelectrons and meanwhile allows us to unambiguously distinguish the main and sideband peaks of the above-threshold ionization spectrum. The relative phase shift between the main and sideband peaks, retrieved from the phase-of-phase of the photoelectron spectrum as a function of the laser phase, gradually decreases with increasing electron energy, and becomes zero for the fast electron which is mainly produced by the rescattering process. Furthermore, a Freeman resonance delay of 140±40 attoseconds between photoelectrons emitted via the 4f and 5p Rydberg states of argon is observed.

  20. Enhanced hole boring with two-color relativistic laser pulses in the fast ignition scheme

    NASA Astrophysics Data System (ADS)

    Yu, Changhai; Deng, Aihua; Tian, Ye; Li, Wentao; Wang, Wentao; Zhang, Zhijun; Qi, Rong; Wang, Cheng; Liu, Jiansheng

    2016-08-01

    A scheme of using two-color laser pulses for hole boring into overdense plasma as well as energy transfer into electron and ion beams has been studied using particle-in-cell simulations. Following an ultra-short ultra-intense hole-boring laser pulse with a short central wavelength in extreme ultra-violet range, the main infrared driving laser pulse can be guided in the hollow channel preformed by the former laser and propagate much deeper into an overdense plasma, as compared to the case using the infrared laser only. In addition to efficiently transferring the main driving laser energy into energetic electrons and ions generation deep inside the overdense plasma, the ion beam divergence can be greatly reduced. The results might be beneficial for the fast ignition concept of inertial confinement fusion.

  1. Design of a real-time two-color interferometer for MAST Upgrade

    SciTech Connect

    O’Gorman, T. Naylor, G.; Scannell, R.; Cunningham, G.; Martin, R.; Croft, D.; Brunner, K. J.

    2014-11-15

    A single chord two-color CO{sub 2}/HeNe (10.6/0.633 μm) heterodyne laser interferometer has been designed to measure the line integral electron density along the mid-plane of the MAST Upgrade tokamak, with a typical error of 1 × 10{sup 18} m{sup −3} (∼2° phase error) at 4 MHz temporal resolution. To ensure this diagnostic system can be restored from any failures without stopping MAST Upgrade operations, it has been located outside of the machine area. The final design and initial testing of this system, including details of the optics, vibration isolation, and a novel phase detection scheme are discussed in this paper.

  2. Two-color multi-section quantum dot distributed feedback laser.

    PubMed

    Naderi, Nader A; Grillot, Frédéric; Yang, Kai; Wright, Jeremy B; Gin, Aaron; Lester, Luke F

    2010-12-20

    A dual-wavelength emission source is realized by asymmetrically pumping a two-section quantum-dot distributed feedback laser. It is found that under asymmetric bias conditions, the powers between the ground-state and excited-state modes of the two-section device can be equalized, which is mainly attributed to the unique carrier dynamics of the quantum-dot gain medium. As a result, a two-color emission with an 8-THz frequency difference is realized that has potential as a compact THz source. It is also shown that the combination of significant inhomogeneous broadening and excited-state coupled mode operation allows the manipulation of the quantum-dot states through external optical stabilization.

  3. Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila.

    PubMed

    Gambis, Alexis; Dourlen, Pierre; Steller, Hermann; Mollereau, Bertrand

    2011-03-01

    We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The "Tomato/GFP-FLP/FRT" method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution.

  4. Coulomb effect on photoelectron momentum distributions in orthogonal two-color laser fields

    NASA Astrophysics Data System (ADS)

    Yu, ShaoGang; Wang, YanLan; Lai, XuanYang; Huang, YiYi; Quan, Wei; Liu, XiaoJun

    2016-09-01

    We theoretically investigate the electron momentum distributions in orthogonally polarized two-color pulses with the Coulomb-Volkov distorted-wave approximation (CVA) theory and focus on the role of the Coulomb potential in the electron momentum distributions by comparing the CVA results with the strong-field approximation (SFA) simulations. Our results show that in comparison with the SFA simulations, the electron momentum distributions in CVA are in better agreement with the experimental observations and the time-dependent Schrödinger equation calculations. By analyzing the phase of the dipole moment, we find that the change of the electron momentum distributions in CVA can be ascribed to the different Coulomb corrections of the phases, which give rise to an enhanced contribution from the forward-rescattering electron and, on the other hand, a decrease of the contribution from the direct electron in the presence of the Coulomb potential.

  5. Two-color optical charge-coupled-device-based pyrometer using a two-peak filter

    NASA Astrophysics Data System (ADS)

    Fu, Tairan; Zhao, Huan; Zeng, Jun; Zhong, Maohua; Shi, Congling

    2010-12-01

    A two-color optical charge-coupled-device (CCD)-based pyrometer was developed using a multipeak interference filter with a color CCD sensor to measure multicolor signals with specified wavelengths. The effective and simple method adjusts the fixed spectrum response characteristics of a color CCD to allow improved temperature measurements. This pyrometer system not only has the advantage of traditional two-color (two-wavelength) pyrometry, but also overcomes the restrictions of color CCDs that can only be applied in waveband measurements. The measurement performance of the system using a two-peak filter (λ _1 = 643 nm,λ _2 = 564 nm) was evaluated by blackbody experiments. The results show that the low temperature detection limit is increased about 200 K with an increase in the sensitivity of the measured signals compared with the original system without two-peak filter [Fu, et al., Opt. Laser Technol. 42, 586 (2010)]. And the effective temperature range is also increased when T > 1233 K. The measured ratio C_R /C_G is monotonically relative to the temperature, which simplifies the measurements. The temperature sensitivity of 2.49 is larger and more uniform than the temperature sensitivity of 1.36 in the previous original system. Thus, the measurement performance of the new system is greatly improved. Finally, as an application, the surface temperature distribution of stainless steel sample in hot environments was determined by this new CCD-based pyrometer. The results agree well with the spectrometer-based results and further verify the applicability of the new system.

  6. In Silico Comparative Transcriptome Analysis of Two Color Morphs of the Common Coral Trout (Plectropomus Leopardus)

    PubMed Central

    Wang, Le; Yu, Cuiping; Guo, Liang; Lin, Haoran; Meng, Zining

    2015-01-01

    The common coral trout is one species of major importance in commercial fisheries and aquaculture. Recently, two different color morphs of Plectropomus leopardus were discovered and the biological importance of the color difference is unknown. Since coral trout species are poorly characterized at the molecular level, we undertook the transcriptomic characterization of the two color morphs, one black and one red coral trout, using Illumina next generation sequencing technologies. The study produced 55162966 and 54588952 paired-end reads, for black and red trout, respectively. De novo transcriptome assembly generated 95367 and 99424 unique sequences in black and red trout, respectively, with 88813 sequences shared between them. Approximately 50% of both trancriptomes were functionally annotated by BLAST searches against protein databases. The two trancriptomes were enriched into 25 functional categories and showed similar profiles of Gene Ontology category compositions. 34110 unigenes were grouped into 259 KEGG pathways. Moreover, we identified 14649 simple sequence repeats (SSRs) and designed primers for potential application. We also discovered 130524 putative single nucleotide polymorphisms (SNPs) in the two transcriptomes, supplying potential genomic resources for the coral trout species. In addition, we identified 936 fast-evolving genes and 165 candidate genes under positive selection between the two color morphs. Finally, 38 candidate genes underlying the mechanism of color and pigmentation were also isolated. This study presents the first transcriptome resources for the common coral trout and provides basic information for the development of genomic tools for the identification, conservation, and understanding of the speciation and local adaptation of coral reef fish species. PMID:26713756

  7. Interaction Between Two CMEs During 14 - 15 February 2011 and Their Unusual Radio Signature

    NASA Astrophysics Data System (ADS)

    Shanmugaraju, A.; Prasanna Subramanian, S.; Vrsnak, Bojan; Ibrahim, M. Syed

    2014-12-01

    We report a detailed analysis of an interaction between two coronal mass ejections (CMEs) that were observed on 14 - 15 February 2011 and the corresponding radio enhancement, which was similar to the "CME cannibalism" reported by Gopalswamy et al. ( Astrophys. J. 548, L91, 2001). A primary CME, with a mean field-of-view velocity of 669 km s-1 in the Solar and Heliospheric Observatory (SOHO)/ Large Angle Spectrometric Coronagraph (LASCO), was more than as twice as fast as the slow CME preceding it (326 km s-1), which indicates that the two CMEs interacted. A radio-enhancement signature (in the frequency range 1 MHz - 400 kHz) due to the CME interaction was analyzed and interpreted using the CME data from LASCO and from the Solar Terrestrial Relations Observatory (STEREO) HI-1, radio data from Wind/ Radio and Plasma Wave Experiment (WAVES), and employing known electron-density models and kinematic modeling. The following results are obtained: i) The CME interaction occurred around 05:00 - 10:00 UT in a height range 20 - 25 R⊙. An unusual radio signature is observed during the time of interaction in the Wind/WAVES dynamic radio spectrum. ii) The enhancement duration shows that the interaction segment might be wider than 5 R⊙. iii) The shock height estimated using density models for the radio enhancement region is 10 - 30 R⊙. iv) Using kinematic modeling and assuming a completely inelastic collision, the decrease of kinetic energy based on speeds from LASCO data is determined to be 0.77×1023 J, and 3.67×1023 J if speeds from STEREO data are considered. vi) The acceleration, momentum, and force are found to be a=-168 m s-2, I=6.1×1018 kg m s-1, and F=1.7×1015 N, respectively, using STEREO data.

  8. Hearing and loud music exposure in 14-15 years old adolescents.

    PubMed

    Serra, Mario R; Biassoni, Ester C; Hinalaf, María; Abraham, Mónica; Pavlik, Marta; Villalobo, Jorge Pérez; Curet, Carlos; Joekes, Silvia; Yacci, María R; Righetti, Andrea

    2014-01-01

    Adolescent exposure to loud music has become a social and health problem whose study demands a holistic approach. The aims of the current study are: (1) To detect early noise-induced hearing loss among adolescents and establish its relationship with their participation in musical recreational activities and (2) to determine sound immission levels in nightclubs and personal music players (PMPs). The participants consisted in 172 14-15 years old adolescents from a technical high school. Conventional and extended high frequency audiometry, transient evoked otoacoustic emissions and questionnaire on recreational habits were administered. Hearing threshold levels (HTLs) were classified as: normal (Group 1), slightly shifted (Group 2), and significantly shifted (Group 3). The musical general exposure (MGE), from participation in recreational musical activities, was categorized in low, moderate, and high exposure. The results revealed an increase of HTL in Group 2 compared with Group 1 (P < 0.01), in Group 3 compared with Group 2 (P < 0.05) only in extended high frequency range, in Group 3 compared with Group 1 (P < 0.01). Besides, a decrease in mean global amplitude, reproducibility and in frequencies amplitude in Group 2 compared with Group 1 (P < 0.05) and in Group 3 compared with Group 1 (P < 0.05). A significant difference (P < 0.05) was found in Group 1's HTL between low and high exposure, showing higher HTL in high exposure. The sound immission measured in nightclubs (107.8-112.2) dBA and PMPs (82.9-104.6) dBA revealed sound levels risky for hearing health according to exposure times. It demonstrates the need to implement preventive and hearing health promoting actions in adolescents.

  9. 40 CFR 721.10284 - Poly[oxy(methyl-1,2-ethanediyl)], .alpha.-sulfo-.omega.-hydroxy-, C14-15-branched and linear...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...-, C14-15-branched and linear alkyl ethers, sodium salts. 721.10284 Section 721.10284 Protection of...-.omega.-hydroxy-, C14-15-branched and linear alkyl ethers, sodium salts. (a) Chemical substance and...-.omega.-hydroxy-, C14-15-branched and linear alkyl ethers, sodium salts (PMN P-10-487; CAS No....

  10. Two-color method for optical astrometry - Theory and preliminary measurements with the Mark III stellar interferometer

    NASA Technical Reports Server (NTRS)

    Colavita, M. Mark; Shao, Michael; Staelin, David H.

    1987-01-01

    The two-color method for interferometric astrometry provides a means of reducing the error in a stellar position measurement attributable to atmospheric turbulence. The primary limitation of the method is shown to be turbulent water vapor fluctuations. Secondary atmospheric effects caused by diffraction from small refractive-index inhomogeneities and differential refraction for the observation of stars away from zenith are shown to introduce errors that behave as white noise and which should not be significant. Other potential error sources due to photon noise, systematic instrumental effects, and imperfect data reduction are also considered. The improvement in accuracy possible with the two-color method is estimated as a factor of 5-10 over the corresponding one-color measurement. Some preliminary two-color measurements with the Mark III stellar interferometer at Mt. Wilson are presented, which demonstrate a factor of about 5 reduction in the amplitude of the atmospheric fluctuations in a stellar position measurement.

  11. Two-color RESOLFT nanoscopy with green and red fluorescent photochromic proteins.

    PubMed

    Lavoie-Cardinal, Flavie; Jensen, Nickels A; Westphal, Volker; Stiel, Andre C; Chmyrov, Andriy; Bierwagen, Jakob; Testa, Ilaria; Jakobs, Stefan; Hell, Stefan W

    2014-03-17

    Up to now, all demonstrations of reversible saturable optical fluorescence transitions (RESOLFT) superresolution microscopy of living cells have relied on the use of reversibly switchable fluorescent proteins (RSFP) emitting in the green spectral range. Here we show RESOLFT imaging with rsCherryRev1.4, a new red-emitting RSFP enabling a spatial resolution up to four times higher than the diffraction barrier. By co-expressing green and red RSFPs in living cells we demonstrate two-color RESOLFT imaging both for single ("donut") beam scanning and for parallelized versions of RESOLFT nanoscopy where an array of >23,000 "donut-like" minima are scanned simultaneously. ©2014 The Authors. Published by Wiley-VCH Verlag GmbH&Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

  12. Enhanced sensitivity in H photofragment detection by two-color reduced-Doppler ion imaging

    NASA Astrophysics Data System (ADS)

    Epshtein, Michael; Portnov, Alexander; Kupfer, Rotem; Rosenwaks, Salman; Bar, Ilana

    2013-11-01

    Two-color reduced-Doppler (TCRD) and one-color velocity map imaging (VMI) were used for probing H atom photofragments resulting from the ˜243.1 nm photodissociation of pyrrole. The velocity components of the H photofragments were probed by employing two counterpropagating beams at close and fixed wavelengths of 243.15 and 243.12 nm in TCRD and a single beam at ˜243.1 nm, scanned across the Doppler profile in VMI. The TCRD imaging enabled probing of the entire velocity distribution in a single pulse, resulting in enhanced ionization efficiency, as well as improved sensitivity and signal-to-noise ratio. These advantages were utilized for studying the pyrrole photodissociation at ˜243.1 and 225 nm, where the latter wavelength provided only a slight increase in the H yield over the self-signal from the probe beams. The TCRD imaging enabled obtaining high quality H+ images, even for the low H photofragment yields formed in the 225 nm photolysis process, and allowed determining the velocity distributions and anisotropy parameters and getting insight into pyrrole photodissociation.

  13. Two-color temporal focusing multiphoton excitation imaging with tunable-wavelength excitation

    NASA Astrophysics Data System (ADS)

    Lien, Chi-Hsiang; Abrigo, Gerald; Chen, Pei-Hsuan; Chien, Fan-Ching

    2017-02-01

    Wavelength tunable temporal focusing multiphoton excitation microscopy (TFMPEM) is conducted to visualize optical sectioning images of multiple fluorophore-labeled specimens through the optimal two-photon excitation (TPE) of each type of fluorophore. The tunable range of excitation wavelength was determined by the groove density of the grating, the diffraction angle, the focal length of lenses, and the shifting distance of the first lens in the beam expander. Based on a consideration of the trade-off between the tunable-wavelength range and axial resolution of temporal focusing multiphoton excitation imaging, the presented system demonstrated a tunable-wavelength range from 770 to 920 nm using a diffraction grating with groove density of 830 lines/mm. TPE fluorescence imaging examination of a fluorescent thin film indicated that the width of the axial confined excitation was 3.0±0.7 μm and the shifting distance of the temporal focal plane was less than 0.95 μm within the presented wavelength tunable range. Fast different wavelength excitation and three-dimensionally rendered imaging of Hela cell mitochondria and cytoskeletons and mouse muscle fibers were demonstrated. Significantly, the proposed system can improve the quality of two-color TFMPEM images through different excitation wavelengths to obtain higher-quality fluorescent signals in multiple-fluorophore measurements.

  14. THz generation from elliptically-focused two-color laser pulses at 1 kHz

    NASA Astrophysics Data System (ADS)

    Yoo, Yungjun; Kuk, Donghoon; Zhong, Zheqiang; Kim, Ki-Yong

    2017-01-01

    We have generated high-power terahertz (THz) radiation by elliptical focusing of two-color femtosecond laser pulses in air at a 1-kHz repetition rate. Elliptical focusing produces a 2-dimensional plasma sheet, emitting two diverging THz radiation lobes in the far field. Such radiation is collimated and refocused by a combination of cylindrical and off-axis parabolic mirrors. Here the distances between the 2-D plasma sheet, cylindrical mirror, and off-axis parabolic mirror are carefully adjusted to minimize the THz spot size at the refocus. The refocused THz field strength is estimated by measuring the THz energy, beam spot size, and waveform. Here an uncooled microbolometer camera with real-time lock-in imaging is used to monitor and measure the focused THz beam profiles with a high signal-to-noise ratio at a broad range of THz (1 40 THz) frequencies. High-pressure gas (N2 and Ar) jets puffed in air are also tested as laser targets to boost the output THz energy even further. This material is based upon work supported by the Air Force Office of Scientific Research under Award Number FA9550-16-1-0163.

  15. Strong-field ionization with two-color circularly polarized laser fields

    NASA Astrophysics Data System (ADS)

    Mancuso, Christopher A.; Hickstein, Daniel D.; Grychtol, Patrik; Knut, Ronny; Kfir, Ofer; Tong, Xiao-Min; Dollar, Franklin; Zusin, Dmitriy; Gopalakrishnan, Maithreyi; Gentry, Christian; Turgut, Emrah; Ellis, Jennifer L.; Chen, Ming-Chang; Fleischer, Avner; Cohen, Oren; Kapteyn, Henry C.; Murnane, Margaret M.

    2015-03-01

    Strong-field ionization provides fundamental insight into light-matter interactions, encoding the structure of atoms and molecules on the subångström and subfemtosecond scales. In this Rapid Communication, we explore an important regime: strong-field ionization by two-color circularly polarized laser fields. In contrast to past work using linearly polarized drivers, we probe electron trajectories that are driven in a two-dimensional plane, thus separating the tunneling angle from the rescattering angle. This allows us to make several findings. First, we observe a single-lobed electron distribution for co-rotating fields, and a three-lobed distribution for counter-rotating fields, providing experimental validation of the theoretical model explaining the generation of circularly polarized high harmonic light. Second, we discover that there is significant electron-ion rescattering using counter-rotating fields, but not with co-rotating fields. Finally, we show that the rescattered electrons are well separated from the directly ionized electrons, in striking contrast to similar low-energy structures seen with linearly polarized fields. These findings help overcome the long-standing problem of how to decouple the tunneling and rescattering steps in strong-field ionization, which will enable new dynamic probes of atomic and molecular structure.

  16. Enhanced sensitivity in H photofragment detection by two-color reduced-Doppler ion imaging

    SciTech Connect

    Epshtein, Michael; Portnov, Alexander; Kupfer, Rotem; Rosenwaks, Salman; Bar, Ilana

    2013-11-14

    Two-color reduced-Doppler (TCRD) and one-color velocity map imaging (VMI) were used for probing H atom photofragments resulting from the ∼243.1 nm photodissociation of pyrrole. The velocity components of the H photofragments were probed by employing two counterpropagating beams at close and fixed wavelengths of 243.15 and 243.12 nm in TCRD and a single beam at ∼243.1 nm, scanned across the Doppler profile in VMI. The TCRD imaging enabled probing of the entire velocity distribution in a single pulse, resulting in enhanced ionization efficiency, as well as improved sensitivity and signal-to-noise ratio. These advantages were utilized for studying the pyrrole photodissociation at ∼243.1 and 225 nm, where the latter wavelength provided only a slight increase in the H yield over the self-signal from the probe beams. The TCRD imaging enabled obtaining high quality H{sup +} images, even for the low H photofragment yields formed in the 225 nm photolysis process, and allowed determining the velocity distributions and anisotropy parameters and getting insight into pyrrole photodissociation.

  17. Two-color detector: Mercury-cadmium-telluride as a terahertz and infrared detector

    SciTech Connect

    Sizov, F.; Zabudsky, V.; Petryakov, V.; Golenkov, A.; Andreyeva, K.; Tsybrii, Z.; Dvoretskii, S.

    2015-02-23

    In this paper, issues associated with the development of infrared (IR) and terahertz (THz) radiation detectors based on HgCdTe are discussed. Two-color un-cooled and cooled to 78 K narrow-gap mercury-cadmium-telluride semiconductor thin layers with antennas were considered both as sub-THz (sub-THz) direct detection bolometers and 3–10 μm IR photoconductors. The noise equivalent power (NEP) for one of the detectors studied at ν ≈ 140 GHz reaches NEP{sub 300 K} ≈ 4.5 × 10{sup −10} W/Hz{sup 1/2} and NEP{sub 78 K} ≈ 5 × 10{sup −9} W/Hz{sup 1/2}. The same detector used as an IR photoconductor showed the responsivity at temperatures T = 78 K and 300 K with signal-to-noise ratio S/N ≈ 750 and 50, respectively, under illumination by using IR monochromator and globar as a thermal source.

  18. Multi-photon two-color ionization of atoms and ions by femtosecond pulses

    NASA Astrophysics Data System (ADS)

    Douguet, Nicolas; Venzke, Joel; Bartschat, Klaus; Grum-Grzhimailo, Alexei N.; Gryzlova, Elena; Staroselskaya, Ekaterina

    2016-05-01

    We consider several processes related to two-color ionization induced by femtosecond pulses. Using the first and second harmonics of an XUV pulse, one can produce two-pathway interferences, which directly influence the photoelectron angular distribution. We discuss the process with linearly as well as circularly polarized light of various mutual orientations and helicities. Furthermore, combining the XUV light with an optical laser, one can generate sidebands around the main photoelectron line and study a variety of asymmetries in photoelectron emission and their dependencies on the absolute and relative intensities, time delay, and polarization of the light. Calculations for atomic hydrogen, He+(1s) generated by an initial XUV pulse, and Ne(2p) were performed by directly solving the time-dependent Schrödinger equation as well as employing second-order nonstationary perturbation theory. Our predictions serve as guidelines for experiments at various X-ray Free-Electron Laser facilities, such as LCLS, FERMI, FLASH, and the European XFEL. Supported by the NSF under PHY-1430245 and XSEDE PHY-090031.

  19. Differences in Bacterial Community Structure in Two Color Morphs of the Hawaiian Reef Coral Montipora capitata.

    PubMed

    Shore-Maggio, Amanda; Runyon, Christina M; Ushijima, Blake; Aeby, Greta S; Callahan, Sean M

    2015-10-01

    Corals harbor diverse bacterial associations that contribute to the health of the host. Using 16S rRNA pyrosequencing, we compared the bacterial communities of red and orange morphs of the Hawaiian coral Montipora capitata. Although both color morphs shared dominant bacterial genera, weighted and unweighted UniFrac analyses showed distinct bacterial communities. A single operational taxonomic unit (OTU), classified as Vibrio, represented the largest driver of differences between the color morphs. This OTU comprised 35.4% (±5.5%) of the orange morph bacterial community yet comprised 1.1% (±0.6%) of the red morph bacterial community. Cultivable bacteria from the two color morphs were also compared and tested for antibacterial activity. Cultured isolates represented 14 genera (7% of the total genera identified from sequencing data), and all but two cultured isolates had a matching OTU from the sequencing data. Half of the isolates tested (8 out of 16) displayed antibacterial activity against other cultured isolates but not against two known bacterial pathogens of M. capitata. The results from this study demonstrate that the specificity of coral-bacterial associations extends beyond the level of coral species. In addition, culture-dependent methods captured bacterial diversity that was representative of both rare and abundant members of the associated bacterial community, as characterized by culture-independent methods.

  20. A New Catalog of AGB Stars Based on Infrared Two-Color Diagrams

    NASA Astrophysics Data System (ADS)

    Suh, Kyung-Won; Hong, Jinju

    2017-08-01

    We present a new catalog of AGB stars based on infrared two-color diagrams (2CDs) and known properties of the pulsations and spectra. We exclude some misclassified objects from previous catalogs. We identify color areas in two IR 2CDs where most O-rich and C-rich objects listed in previous catalogs of AGB stars are found. By collecting new objects in these color selection areas in the two IR 2CDs, we find candidate objects for AGB stars. By using the color selection method, we identify 3996 new objects in the O-rich areas, 1487 new objects in the C-rich areas, and 295 new objects in the overlap areas of the two 2CDs simultaneously. We have found that 470 O-rich and 9 C-rich objects are Mira variables with positive spectral identification and they are newly identified AGB stars. We present a new catalog of 3828 O-rich AGB stars and 1168 C-rich AGB stars excluding misclassified objects and adding newly identified objects.

  1. Phase diagram of dense two-color QCD within lattice simulations

    NASA Astrophysics Data System (ADS)

    Braguta, V. V.; Ilgenfritz, E.-M.; Kotov, A. Yu.; Molochkov, A. V.; Nikolaev, A. A.

    2017-03-01

    We present the results of a low-temperature scan of the phase diagram of dense two-color QCD with Nf = 2 quarks. The study is conducted using lattice simulation with rooted staggered quarks. At small chemical potential we observe the hadronic phase, where the theory is in a confining state, chiral symmetry is broken, the baryon density is zero and there is no diquark condensate. At the critical point μ = mπ/2 we observe the expected second order transition to Bose-Einstein condensation of scalar diquarks. In this phase the system is still in confinement in conjunction with nonzero baryon density, but the chiral symmetry is restored in the chiral limit. We have also found that in the first two phases the system is well described by chiral perturbation theory. For larger values of the chemical potential the system turns into another phase, where the relevant degrees of freedom are fermions residing inside the Fermi sphere, and the diquark condensation takes place on the Fermi surface. In this phase the system is still in confinement, chiral symmetry is restored and the system is very similar to the quarkyonic state predicted by SU(Nc) theory at large Nc.

  2. INFRARED TWO-COLOR DIAGRAMS FOR AGB STARS, POST-AGB STARS, AND PLANETARY NEBULAE

    SciTech Connect

    Suh, Kyung-Won

    2015-08-01

    We present various infrared two-color diagrams (2CDs) for asymptotic giant branch (AGB) stars, post-AGB stars, and Planetary Nebulae (PNe) and investigate possible evolutionary tracks. We use catalogs from the available literature for the sample of 4903 AGB stars (3373 O-rich; 1168 C-rich; 362 S-type), 660 post-AGB stars (326 post-AGB; 334 pre-PN), and 1510 PNe in our Galaxy. For each object in the catalog, we cross-identify the IRAS, AKARI, Midcourse Space Experiment, and 2MASS counterparts. The IR 2CDs can provide useful information about the structure and evolution of the dust envelopes as well as the central stars. To find possible evolutionary tracks from AGB stars to PNe on the 2CDs, we investigate spectral evolution of post-AGB stars by making simple but reasonable assumptions on the evolution of the central star and dust shell. We perform radiative transfer model calculations for the detached dust shells around evolving central stars in the post-AGB phase. We find that the theoretical dust shell model tracks using dust opacity functions of amorphous silicate and amorphous carbon roughly coincide with the densely populated observed points of AGB stars, post-AGB stars, and PNe on various IR 2CDs. Even though some discrepancies are inevitable, the end points of the theoretical post-AGB model tracks generally converge in the region of the observed points of PNe on most 2CDs.

  3. Dy 3 + -doped yttrium complex molecular crystals for two-color thermometry in heterogeneous materials

    NASA Astrophysics Data System (ADS)

    Anderson, Benjamin R.; Gunawidjaja, Ray; Eilers, Hergen

    2017-08-01

    We develop Dy$^{3+}$-doped yttrium complexes for use as two-color thermometry (TCT) phosphor molecular crystals in heterogeneous materials. These complexes include: Dy:Y(acac)$_3$(phen), Dy:Y(hfa)$_3$(DPEPO), Dy:Y(4-BBA)$_3$(TPPO), Dy:Y(acac)$_3$, and Dy:Y(acac)$_3$(DPEPO), where the Dy/Y ratio is 1:9. We characterize the materials' photoluminescence at different temperatures to determine the TCT calibration parameters and the degree to which thermal quenching influences the emission. From this data we observe a link between the excited state lifetime at room temperature and the degree to which the material is susceptible to thermal quenching (i.e. materials having long room temperature lifetimes are more resistant to thermal quenching than materials with short room temperature lifetimes). Of the five complexes tested we find that Dy:Y(acac)$_3$(DPEPO) has the best thermal performance, with the most likely source of improvement being DPEPO's compact rigid structure. This rigidity helps with energy transfer to the Dy$^{3+}$ ion, suppresses non-radiative loss modes, and reduces exciplex formation.

  4. Two-color fluorescent cytosine extension assay for the determination of global DNA methylation.

    PubMed

    Zhou, Gu; Parfett, Craig; Cummings-Lorbetskie, Cathy; Xiao, Gong-Hua; Desaulniers, Daniel

    2017-04-01

    Here, we present a DNA restriction enzyme-based, fluorescent cytosine extension assay (CEA) to improve normalization and technical variation among sample-to-sample measurements. The assay includes end-labeling of parallel methylation-sensitive and methylation-insensitive DNA restriction enzyme digests along with co-purification and subsequent co-measurement of incorporated fluorescence. This non-radioactive, two-color fluorescent CEA (TCF-CEA) was shown to be a relatively rapid and accurate, with 3-fold greater precision than the one-color CEA. In addition, TCF-CEA provided an index of global DNA methylation that was sensitive to differences >5%. TCF-CEA results were highly correlated with LUminometric Methylation Assay (LUMA) results using human liver cell lines (HepG2, HepaRG, HC-04) as well as a human liver primary cell culture. Hypomethylation was observed in cells treated with the de-methylating agent 5-aza-2'-deoxycytidine. These results demonstrate that TCF-CEA provides a simple method for measuring relative degrees of global DNA methylation that could potentially be scaled up to higher-throughput formats.

  5. Detection of an early cytomegalovirus antigen with two-color quantitative flow cytometry.

    PubMed

    Elmendorf, S; McSharry, J; Laffin, J; Fogleman, D; Lehman, J M

    1988-05-01

    An early cytomegalovirus (CMV) antigen was detected with a monoclonal antibody by two-color fluorescent flow cytometry. With the aid of a human diploid fibroblast cell strain, FLOW 2000, infected with the AD169 strain of CMV, the viral antigen and the DNA content of infected or uninfected cells were measured. There was no evidence of change in the cell-cycle distribution of the infected cells. The viral antigen was detected within 30 minutes following virus adsorption at 0.1 and 1.0 plaque-forming units/cells; and the percentage of positive cells increased with time and viral dosage. All stages of the cell cycle were susceptible to viral infection and the average fluorescence was greater than the background fluorescence. Flow cytometry detected the viral antigen earlier than conventional immunofluorescent microscopy and cell culture for CMV cytopathological effect (CPE). Ten bronchoalveolar lavages assayed by flow cytometry and conventional diagnostic procedures demonstrated that flow cytometry might be useful in early diagnosis for CMV infection.

  6. Two-color mid-infrared thermometer using a hollow glass optical fiber

    SciTech Connect

    Small, W., IV.; Celliers, P.M.; Da Silva, L.D.; Matthews, D.L.

    1997-06-30

    A non-invasive two-color infrared thermometer has been developed for low-temperature biomedical applications. Mid-infrared radiation from the target is collected via a single 700 {mu}m-bore hollow glass optical fiber, simultaneously split into two paths and modulated by a gold-coated reflective optical chopper, and focused onto two thermoelectrically-cooled HgCdZnTe photoconductors (bandpasses of 2- 6 {mu}m and 2-12 {mu}m, respectively) by gold-coated spherical mirrors. The small numerical aperture of the hollow glass fiber provides high spatial resolution (is less than 1 mm), and the hollow bore eliminates reflective losses. The modulated detector signals are recovered using lock-in amplification, permitting measurement of small low-temperature signal buried in the background. A computer algorithm calculates the true temperature and emissivity of the target in real time based on a previous blackbody (emissivity equal to 1) calibration, taking into account reflection of the ambient radiation field from the target surface.

  7. Laser-induced plasmas in air studied using two-color interferometry

    NASA Astrophysics Data System (ADS)

    Yang, Zefeng; Wu, Jian; Wei, Wenfu; Li, Xingwen; Han, Jiaxun; Jia, Shenli; Qiu, Aici

    2016-08-01

    Temporally and spatially resolved density profiles of Cu atoms, electrons, and compressed air, from laser-induced copper plasmas in air, are measured using fast spectral imaging and two-color interferometry. From the intensified CCD images filtered by a narrow-band-pass filter centered at 515.32 nm, the Cu atoms expansion route is estimated and used to determine the position of the fracture surface between the Cu atoms and the air. Results indicate that the Cu atoms density at distances closer to the target (0-0.4 mm) is quite low, with the maximum density appearing at the edge of the plasma's core being ˜4.6 × 1024 m-3 at 304 ns. The free electrons are mainly located in the internal region of the plume, which is supposed to have a higher temperature. The density of the shock wave is (4-6) × 1025 m-3, corresponding to air compression of a factor of 1.7-2.5.

  8. Multistimuli two-color luminescence switching via different slip-stacking of highly fluorescent molecular sheets.

    PubMed

    Yoon, Seong-Jun; Chung, Jong Won; Gierschner, Johannes; Kim, Kil Suk; Choi, Moon-Gun; Kim, Dongho; Park, Soo Young

    2010-10-06

    Color tuning and switching of the solid-state luminescence of organic materials are attractive subjects for both the fundamental research and practical applications such as optical recording. We report herein cyanostilbene-based highly luminescent molecular sheets which exhibit two-color fluorescence switching in response to pressure, temperature, and solvent vapor. The origin for the multistimuli luminescence switching is the two-directional shear-sliding capability of molecular sheets, which are formed via intermolecular multiple C-H···N and C-H···O hydrogen bonds. The resulting two distinctive crystal phases are promoted by different modes of local dipole coupling, which cause a substantial alternation of π-π overlap. These changes can be directly correlated with the subsequent intermolecular excitonic and excimeric coupling in both phases, as demonstrated by an in-depth theory-assisted spectroscopic and structural study. Finally, we have prepared a first device demonstrator for rewritable fluorescent optical recording media which showed multistimuli luminescence tuning with fast response. Our multistimuli responsive system is unique in terms of the slip-stacking of molecular sheets and thus provides a novel concept of rewritable fluorescent optical recording media.

  9. Bias Selective Operation of Sb-Based Two-Color Photodetectors

    NASA Technical Reports Server (NTRS)

    Abedin, M. N.; Refaat, Tamer F.; Bhat, Ishwara B.; Xiao, Yegao; Johnson, David G.

    2006-01-01

    Multicolor detectors have a strong potential to replace conventional single-color detectors in application dealing with the simultaneous detection of more than one wavelength. This will lead to the reduction of heavy and complex optical components now required for spectral discrimination for multi-wavelengths applications. This multicolor technology is simpler, lighter, compact and cheaper with respect to the single-color ones. In this paper, Sb-based two-color detectors fabrication and characterization are presented. The color separation is achieved by fabricating dual band pn junction on a GaSb substrate. The first band consists of an InGaAsSb pn junction for long wavelength detection, while the second band consists of a GaSb pn junction for shorter wavelength detection. Three metal contacts were deposited to access the individual junctions. Surface morphology of multi-layer thin films and also device characteristics of quasi-dual band photodetector were characterized using standard optical microscope and electro-optic techniques respectively. Dark current measurements illustrated the diode behavior of both lattice-matched detector bands. Spectral response measurements indicated either independent operation of both detectors simultaneously, or selective operation of one detector, by the polarity of the bias voltage, while serially accessing both devices.

  10. Laser-induced plasmas in air studied using two-color interferometry

    SciTech Connect

    Yang, Zefeng; Wu, Jian Li, Xingwen; Han, Jiaxun; Jia, Shenli; Qiu, Aici; Wei, Wenfu

    2016-08-15

    Temporally and spatially resolved density profiles of Cu atoms, electrons, and compressed air, from laser-induced copper plasmas in air, are measured using fast spectral imaging and two-color interferometry. From the intensified CCD images filtered by a narrow-band-pass filter centered at 515.32 nm, the Cu atoms expansion route is estimated and used to determine the position of the fracture surface between the Cu atoms and the air. Results indicate that the Cu atoms density at distances closer to the target (0–0.4 mm) is quite low, with the maximum density appearing at the edge of the plasma's core being ∼4.6 × 10{sup 24 }m{sup −3} at 304 ns. The free electrons are mainly located in the internal region of the plume, which is supposed to have a higher temperature. The density of the shock wave is (4–6) × 10{sup 25 }m{sup −3}, corresponding to air compression of a factor of 1.7–2.5.

  11. Multiscale Monte Carlo equilibration: Two-color QCD with two fermion flavors

    NASA Astrophysics Data System (ADS)

    Detmold, William; Endres, Michael G.

    2016-12-01

    We demonstrate the applicability of a recently proposed multiscale thermalization algorithm to two-color quantum chromodynamics (QCD) with two mass-degenerate fermion flavors. The algorithm involves refining an ensemble of gauge configurations that had been generated using a renormalization group (RG) matched coarse action, thereby producing a fine ensemble that is close to the thermalized distribution of a target fine action; the refined ensemble is subsequently rethermalized using conventional algorithms. Although the generalization of this algorithm from pure Yang-Mills theory to QCD with dynamical fermions is straightforward, we find that in the latter case, the method is susceptible to numerical instabilities during the initial stages of rethermalization when using the hybrid Monte Carlo algorithm. We find that these instabilities arise from large fermion forces in the evolution, which are attributed to an accumulation of spurious near-zero modes of the Dirac operator. We propose a simple strategy for curing this problem, and demonstrate that rapid thermalization—as probed by a variety of gluonic and fermionic operators—is possible with the use of this solution. In addition, we study the sensitivity of rethermalization rates to the RG matching of the coarse and fine actions, and identify effective matching conditions based on a variety of measured scales.

  12. Study of the phase diagram of dense two-color QCD within lattice simulation

    NASA Astrophysics Data System (ADS)

    Braguta, V. V.; Ilgenfritz, E.-M.; Kotov, A. Yu.; Molochkov, A. V.; Nikolaev, A. A.

    2016-12-01

    In this paper, we carry out a low-temperature scan of the phase diagram of dense two-color QCD with Nf=2 quarks. The study is conducted using lattice simulation with rooted staggered quarks. At small chemical potential, we observe the hadronic phase, where the theory is in a confining state, chiral symmetry is broken, the baryon density is zero, and there is no diquark condensate. At the critical point μ =mπ/2 , we observe the expected second-order transition to Bose-Einstein condensation of scalar diquarks. In this phase, the system is still in confinement in conjunction with nonzero baryon density, but the chiral symmetry is restored in the chiral limit. We have also found that in the first two phases the system is well described by chiral perturbation theory. For larger values of the chemical potential, the system turns into another phase, where the relevant degrees of freedom are fermions residing inside the Fermi sphere, and the diquark condensation takes place on the Fermi surface. In this phase, the system is still in confinement, chiral symmetry is restored, and the system is very similar to the quarkyonic state predicted by SU (Nc) theory at large Nc.

  13. Gradient effects on two-color soot optical pyrometry in a heavy-duty DI diesel engine

    SciTech Connect

    Musculus, Mark P.B.; Singh, Satbir; Reitz, Rolf D.

    2008-04-15

    Two-color soot optical pyrometry is a widely used technique for measuring soot temperature and volume fraction in many practical combustion devices, but line-of-sight soot temperature and volume fraction gradients can introduce significant uncertainties in the measurements. For diesel engines, these uncertainties usually can only be estimated based on assumptions about the soot property gradients along the line of sight, because full three-dimensional transient diesel soot distribution data are not available. Such information is available, however, from multidimensional computer model simulations, which are phenomenologically based, and have been validated against available in-cylinder soot measurements and diesel engine exhaust soot emissions. Using the model-predicted in-cylinder soot distributions, uncertainties in diesel two-color pyrometry data are assessed, both for a conventional high-sooting, high-temperature combustion (HTC) operating condition, and for a low-sooting, low-temperature combustion (LTC) condition. The simulation results confirm that the two-color soot measurements are strongly biased toward the properties of the hot soot. For the HTC condition, line-of-sight gradients in soot temperature span 600 K, causing relatively large errors. The two-color temperature is 200 K higher than the soot-mass-averaged value, while the two-color volume fraction is 50% lower. For the LTC condition, the two-color measurement errors are half as large as for the HTC condition, because the model-predicted soot temperature gradients along the line of sight are half as large. By contrast, soot temperature and volume fraction gradients across the field of view introduce much smaller errors of less than 50 K in temperature and 20% in volume fraction. (author)

  14. Grating-based wavelength control of single- and two-color vertical-external-cavity-surface-emitting lasers.

    PubMed

    Scheller, Maik; Koch, Stephan W; Moloney, Jerome V

    2012-01-01

    Wide wavelength tunability of single- and two-color operating vertical-external-cavity-surface-emitting lasers (VECSELs) is demonstrated. Employing an external feedback based on a diffractive grating outside the cavity of a narrow-line single-color VECSEL allows for a continuous tuning of the emission wavelength over 10 nm. Employing a dual-feedback-configuration for tunable two-color emission, a tunability of the difference frequency between the two lasing wavelengths from 300 gigahertz to up to 3.5 terahertz is demonstrated.

  15. Two-color field for the generation of an isolated attosecond pulse in water-window region.

    PubMed

    Chen, Wenxiang; Chen, Guanglong; Kim, Dong Eon

    2011-10-10

    For the investigation of various ultrafast electron dynamics, an isolated attosecond pulse in a broad spectral range is necessary. The generation of isolated attosecond pulses demands the manipulation of the electric field of a laser. We propose a two-color field scheme for generating an isolated attosecond pulse in the water-window region. Two-color fields are generated by mixing two equally-strong pulsed color fields. The investigation shows that an isolated attosecond pulse with a photon energy of near 500 eV and a pulse duration of 125 - 188 attoseconds can be generated using 10 - 15 fs FWHM laser fields.

  16. Significant Influences on Nature Experiences: A Comparative Study of Southern German and Northern Spanish Pupils Aged 14-15

    ERIC Educational Resources Information Center

    Zecha, Stefanie

    2015-01-01

    An understanding of the current situation of nature experiences among adolescents is of critical importance for the protection of nature. The participants of this study are 14-15 year-old pupils from Bavaria (Southern Germany) and Asturias (Northern Spain). In particular, the author was interested in a) the level of outdoor experiences of each…

  17. Significant Influences on Nature Experiences: A Comparative Study of Southern German and Northern Spanish Pupils Aged 14-15

    ERIC Educational Resources Information Center

    Zecha, Stefanie

    2015-01-01

    An understanding of the current situation of nature experiences among adolescents is of critical importance for the protection of nature. The participants of this study are 14-15 year-old pupils from Bavaria (Southern Germany) and Asturias (Northern Spain). In particular, the author was interested in a) the level of outdoor experiences of each…

  18. Three-micron survey of Jupiter Trojan asteroids

    NASA Astrophysics Data System (ADS)

    Brown, M.

    2014-07-01

    The Jupiter Trojans, orbiting at the transition between the inner and outer solar system and co-orbiting with the dominant solar system planet, are critical to understanding the formation and dynamical evolution of this critical region of the solar system. One important clue to their formation location should be their composition, but little about this composition is known: the Trojans are spectrally featureless up to 2.5 microns, with either slightly-red or red visible spectral slopes and less steep near-infrared slopes. This bifurcation into two separate spectral classes could be an important clue into the formation location (or locations) of these objects, but, again, with little other spectral information known it is difficult to use this constraint appropriately. Longer wavelength reflectance spectroscopy has the potential to solve this dilemma. Many ices and organic rich materials have their much stronger fundamental absorptions in the 2.8--4 microns region, making this spectral region sensitive to much smaller amounts of these materials. Hydrated silicates, too, have clearly distinguishing features at these wavelengths. We have thus begun a Keck Observatory/NIRSPEC 2.8--4 micron survey of Jupiter Trojans designed to detect these fundamental absorption features. Our goal is to collect a sample of approximately a dozen asteroids in each of the slightly red and red spectral classes. We will compare the inferred compositions both to the outer main-belt asteroids, which have been extensively surveyed in this region, as well as to our knowledge of compositions of small Kuiper-belt objects which, intriguingly, also come in two color classes. We will present the first results from our survey and discuss the implications for the compositions of these objects and the chemical and dynamical evolution of this middle region of the solar system.

  19. One- and Two-Color Resonant Photoionization Spectroscopy of Chromium-Doped Helium Nanodroplets

    PubMed Central

    2014-01-01

    We investigate the photoinduced relaxation dynamics of Cr atoms embedded into superfluid helium nanodroplets. One- and two-color resonant two-photon ionization (1CR2PI and 2CR2PI, respectively) are applied to study the two strong ground state transitions z7P2,3,4° ← a7S3 and y7P2,3,4° ← a7S3. Upon photoexcitation, Cr* atoms are ejected from the droplet in various excited states, as well as paired with helium atoms as Cr*–Hen exciplexes. For the y7P2,3,4° intermediate state, comparison of the two methods reveals that energetically lower states than previously identified are also populated. With 1CR2PI we find that the population of ejected z5P3° states is reduced for increasing droplet size, indicating that population is transferred preferentially to lower states during longer interaction with the droplet. In the 2CR2PI spectra we find evidence for generation of bare Cr atoms in their septet ground state (a7S3) and metastable quintet state (a5S2), which we attribute to a photoinduced fast excitation–relaxation cycle mediated by the droplet. A fraction of Cr atoms in these ground and metastable states is attached to helium atoms, as indicated by blue wings next to bare atom spectral lines. These relaxation channels provide new insight into the interaction of excited transition metal atoms with helium nanodroplets. PMID:24708058

  20. Detection of the AML translocation (8;21) by two-color fluorescent in situ hybridization

    SciTech Connect

    Sacchi, N.; Magnani, I.; Kearney, L.

    1994-09-01

    In the translocation (8;21)(q22;q22) associated with acute myelogenous leukemia (AML), part of the long arm of chromosome 8 is reciprocally translocated onto chromosome 21. At the molecular level the translocation results in the fusion of the 5{prime} region of the AML1 gene on chromosome 21 and almost the entire CDR gene (also ETO or MTG8) on chromosome 8. To detection the translocation at the single cell level, we used two probes, a cosmid clone containing the first five exons of AML1 and a P1 clone containing the entire CDR gene. Hybridization of the two probes to the distal and proximal sides of the translocation breakpoint was expected to highlight the derivative 8q-chromosome in an interphase cell. To demonstrate the ability to identify the translocation in interphase cells using two-color FISH, these two probes were hybridized simultaneously to a cell line containing the 8;21 translocation, Kasumi-1. Each probe was detected with a different color so that their relationship in the sample could be determined within the same interphase cell. Simultaneous hybridization of the CDR and AML1 probes to interphase Kasumi-1 cells resulted in one orange and one green hybridization signal randomly located in the cell, from the hybridization to the normal 8 and 21 chromosomes, and one orange-green pair of signals from the close hybridization of the two probes to the fusion gene on the derivative 8q-chromosome, indicating the translocation. The translocation was identified by an abnormal pairing of the two differently colored signals in the same interphase cell. This technique allows for the detection of the translocation in all cells, not just those arrested in metaphase, and also permits the analysis of a small number of cells. Therefore, useful information can still be obtained from samples not suited for RT-PCR analysis and conventional cytogenetic techniques.

  1. Two-color medium-infrared scanning interferometer for the Frascati tokamak upgrade fusion test device.

    PubMed

    Canton, A; Innocente, P; Tudisco, O

    2006-12-20

    A scanning beam interferometer installed on the Frascati tokamak upgrade (FTU) experiment is presented. The scanning beam scheme combined with the small dimensions of the beams produces a system with very high spatial resolution: more than 30 adjacent (nonoverlapping) chords sample most of the plasma cross section. A good time resolution is achieved by the use of a proper scanning device, with a scanning frequency >or=8 kHz. Very fast events are measured by three additional fixed lines of sight providing a time resolution >or=100 kHz. The instrument is a two-color medium-infrared-compensated-type interferometer; two wavelengths (colors) are used to measure both the density and the mechanical vibrations of optical components. A CO2 laser (lambda=10.6 microm) is the main light source, and a CO laser (lambda=5.4 microm) is the compensation one. The optical scheme is a double pass Mach-Zehnder type. All the retroreflector mirrors are mounted directly on the FTU mechanical structure thanks to the compensation system that allows for large vibration amplitudes of optical components. Heterodyne detection at 30 and 40 MHz is obtained by frequency shifting the reference beams with two acousto-optic modulators (Bragg cells). Many features are implemented to achieve high measurement accuracy and reliability. A real-time system computes the integral density measured on one of the fixed lines of sight and provides an analog signal for density feedback control. The interferometer was used to measure density profiles both in medium-density discharges (n(e) approximately 10(20) m(-3)) and in high-density pellet injected discharges (n(e) approximately 7-8 x 10(20) m(-3)). The measurement error is approximately 2 x 10(18) m(-2) under optimal conditions but can be higher in some cases, mainly because of the large tilt of the retroreflector mirrors.

  2. Rovibronically selected and resolved two-color laser photoionization and photoelectron study of nickel carbide cation.

    PubMed

    Chang, Yih Chung; Shi, Xiaoyu; Lau, Kai-Chung; Yin, Qing-Zhu; Liou, H T; Ng, C Y

    2010-08-07

    We have performed a two-color laser photoionization and photoelectron study of nickel carbide (NiC) and its cation (NiC(+)). By preparing NiC in a single rovibronic level of an intermediate vibronic state via visible laser excitation prior to ultraviolet laser photoionization, we have measured the photoionization efficiency spectrum of NiC near its ionization threshold, covering the formation of NiC(+)(X (2)Sigma(+);v(+)=0-3). We have also obtained well-resolved rotational transitions for the v(+)=0 and 1 vibrational bands of the NiC(+)(X (2)Sigma(+)) ground state. The assignment of rotational transitions observed between the neutral NiC intermediate state and the NiC(+) ion ground state has allowed the direct determination of a highly precise value for the ionization energy of NiC, IE(NiC)=67,525.1+/-0.5 cm(-1) (8.372 05+/-0.000 06 eV). This experiment also provides reliable values for the vibrational spacing [DeltaG(1/2)=859.5+/-0.5 cm(-1)], rotational constants (B(e)(+)=0.6395+/-0.0018 cm(-1) and alpha(e)(+)=0.0097+/-0.0009 cm(-1)), and equilibrium bond distance (r(e)(+)=1.628 A) for the NiC(+)(X (2)Sigma(+)) ground state. The experimental results presented here are valuable for benchmarking the development of more reliable ab initio quantum computation procedures for energetic and spectroscopic calculations of transition metal-containing molecules.

  3. Rovibronically selected and resolved two-color laser photoionization and photoelectron study of nickel carbide cation

    NASA Astrophysics Data System (ADS)

    Chang, Yih Chung; Shi, Xiaoyu; Lau, Kai-Chung; Yin, Qing-Zhu; Liou, H. T.; Ng, C. Y.

    2010-08-01

    We have performed a two-color laser photoionization and photoelectron study of nickel carbide (NiC) and its cation (NiC+). By preparing NiC in a single rovibronic level of an intermediate vibronic state via visible laser excitation prior to ultraviolet laser photoionization, we have measured the photoionization efficiency spectrum of NiC near its ionization threshold, covering the formation of NiC+(X Σ2+;v+=0-3). We have also obtained well-resolved rotational transitions for the v+=0 and 1 vibrational bands of the NiC+(X Σ2+) ground state. The assignment of rotational transitions observed between the neutral NiC intermediate state and the NiC+ ion ground state has allowed the direct determination of a highly precise value for the ionization energy of NiC, IE(NiC)=67 525.1±0.5 cm-1 (8.372 05±0.000 06 eV). This experiment also provides reliable values for the vibrational spacing [ΔG(1/2)=859.5±0.5 cm-1], rotational constants (Be+=0.6395±0.0018 cm-1 and αe+=0.0097±0.0009 cm-1), and equilibrium bond distance (re+=1.628 Å) for the NiC+(X Σ2+) ground state. The experimental results presented here are valuable for benchmarking the development of more reliable ab initio quantum computation procedures for energetic and spectroscopic calculations of transition metal-containing molecules.

  4. Accuracy of two-color peak-height-encoded DNA sequencing by capillary gel electrophoresis and laser-induced fluorescence

    NASA Astrophysics Data System (ADS)

    Bay, Sue; Starke, Heather; Elliott, John; Dovichi, Norman J.

    1993-06-01

    The two-color peak-height encoded DNA sequencing technique was evaluated with six clones taken from the malaria genome. This technique produced a sequencing accuracy of at least 97.5%. Capillary gel electrophoresis, using a modest voltage of 200 V/cm, showed a three- fold increase in speed and higher efficiency compared to conventional slab gel technique.

  5. Countermeasure effectiveness against a man-portable air-defense system containing a two-color spinscan infrared seeker

    NASA Astrophysics Data System (ADS)

    Jackman, James; Richardson, Mark; Butters, Brian; Walmsley, Roy

    2011-12-01

    Man-portable air-defense (MANPAD) systems have developed sophisticated counter-countermeasures (CCM) to try and defeat any expendable countermeasure that is deployed by an aircraft. One of these is a seeker that is able to detect in two different parts of the electromagnetic spectrum. Termed two-color, the seeker can compare the emissions from the target and a countermeasure in different wavebands and reject the countermeasure. In this paper we describe the modeling process of a two-color infrared seeker using COUNTERSIM, a missile engagement and countermeasure software simulation tool. First, the simulations model a MANPAD with a two-color CCM which is fired against a fast jet model and a transport aircraft model releasing reactive countermeasures. This is then compared to when the aircraft releases countermeasures throughout an engagement up to the hit point to investigate the optimum flare firing time. The results show that the release time of expendable decoys as a countermeasure against a MANPAD with a two-color CCM is critical.

  6. Micronized-Coal Burner Facility

    NASA Technical Reports Server (NTRS)

    Calfo, F. D.; Lupton, M. W.

    1986-01-01

    Micronized-coal (coal-in-oil mix) burner facility developed to fulfill need to generate erosion/corrosion data on series of superalloy specimens. In order to successfully operate gas turbine using COM, two primary conditions must be met. First, there must be adequate atomization of COM and second, minimization of coking of burner. Meeting these conditions will be achieved only by clean burning and flame stability.

  7. Rovibronically selected and resolved two-color laser photoionization and photoelectron study of cobalt carbide cation.

    PubMed

    Huang, Huang; Chang, Yih Chung; Luo, Zhihong; Shi, Xiaoyu; Lam, Chow-Shing; Lau, Kai-Chung; Ng, C Y

    2013-03-07

    We have conducted a two-color visible-ultraviolet (VIS-UV) resonance-enhanced laser photoionization efficiency and pulsed field ionization-photoelectron (PFI-PE) study of gaseous cobalt carbide (CoC) near its ionization onset in the total energy range of 61,200-64,510 cm(-1). The cold gaseous CoC sample was prepared by a laser ablation supersonically cooled beam source. By exciting CoC molecules thus generated to single N' rotational levels of the intermediate CoC∗((2)Σ(+); v') state using a VIS dye laser prior to UV laser photoionization, we have obtained N(+) rotationally resolved PFI-PE spectra for the CoC(+)(X(1)Σ(+); v(+) = 0 and 1) ion vibrational bands free from interference by impurity species except Co atoms produced in the ablation source. The rotationally selected and resolved PFI-PE spectra have made possible unambiguous rotational assignments, yielding accurate values for the adiabatic ionization energy of CoC(X(2)Σ(+)), IE(CoC) = 62,384.3 ± 0.6 cm(-1) (7.73467 ± 0.00007 eV), the vibrational frequency ωe (+) = 985.6 ± 0.6 cm(-1), the anharmonicity constant ωe (+)χe (+) = 6.3 ± 0.6 cm(-1), the rotational constants (Be (+) = 0.7196 ± 0.0005 cm(-1), αe (+) = 0.0056 ± 0.0008 cm(-1)), and the equilibrium bond length re (+) = 1.534 Å for CoC(+)(X(1)Σ(+)). The observation of the N(+) = 0 level in the PFI-PE measurement indicates that the CoC(+) ground state is of (1)Σ(+) symmetry. Large ΔN(+) = N(+) - N' changes up to 6 are observed for the photoionization transitions CoC(+)(X(1)Σ(+); v(+) = 0-2; N(+)) ← CoC∗((2)Σ(+); v'; N' = 6, 7, 8, and 9). The highly precise energetic and spectroscopic data obtained in the present study have served as a benchmark for testing theoretical predictions based on state-of-the-art ab initio quantum calculations at the CCSDTQ∕CBS level of theory as presented in the companion article.

  8. Maternal detection of neonatal jaundice during birth hospitalization using a novel two-color icterometer

    PubMed Central

    Slusher, Tina M.; Imosemi, Donald O.; Emokpae, Abieyuwa A.

    2017-01-01

    Background Mothers are frequently the first to observe the onset of jaundice in their newborn infants before the decision to seek treatment. However, simple-to-use tools that could facilitate early detection of jaundice and assist mothers to seek professional care, especially after hospital discharge, are rare. This study therefore, set out to evaluate the performance of a -two-color icterometer (Bilistrip™) as a possible screening tool for detecting significant jaundice by mothers or care-givers in the first week of life. Methods Prior to discharge, mothers in a maternity hospital were trained to use the Bilistrip™ on the blanched skin of their baby’s nose to ascertain absence (Light Yellow) or presence (Dark Yellow) of significant jaundice. Their babies had transcutaneous bilirubin (TcB) measurements independently, along with total serum bilirubin (TSB) if indicated. The reliability of Bilistrip™ as a screening test for significant jaundice was determined at different TcB and TSB thresholds. The predictive performance of Bilistrip™ was also evaluated with multivariable logistic regression. Results Some 2492 mother-infant pairs were enrolled over 15 months, of which 347 (13.9%) chose Dark Yellow. The mean TcB for Dark Yellow (10mg/dL) was significantly higher (p<0.001) than for Light Yellow (6.1mg/dL). Bilistrip™ showed increasing sensitivity (47.0% - 92.6%) and negative predictive value (NPV) (91.4% - 99.9%) for selected TcB thresholds (≥10mg/dL, ≥12mg/dL, ≥15mg/dL, and ≥17mg/dL). Among neonates with TSB measurements (n = 124), Bilistrip™ was associated also with increasing sensitivity (86.8% - 100%) and NPV (62.5% - 100%). The sensitivity and NPV for detecting neonates requiring phototherapy were 95.8% respectively. Only one of the 24 neonates who required phototherapy was missed by the Bilistrip™. Conclusions Bilistrip™ is a potential decision-making tool for empowering mothers to detect neonates with clinically significant jaundice

  9. High Energy 2-micron Laser Developments

    NASA Technical Reports Server (NTRS)

    Yu, Jirong; Trieu, Bo C.; Petros, Mulugeta; Bai, Yingxin; Petzar, Paul J.; Koch, Grady J.; Singh, Upendra N.; Kavaya, Michael J.

    2007-01-01

    This viewgraph presentation shows the development of 2-micron solid state lasers. The topics covered include: 1) Overview 2-micron solid state lasers; 2) Modeling and population inversion measurement; 3) Side pump oscillator; and 4) One Joule 2-m Laser.

  10. Enhancing high-order-harmonic generation by time delays between two-color, few-cycle pulses

    NASA Astrophysics Data System (ADS)

    Peng, Dian; Pi, Liang-Wen; Frolov, M. V.; Starace, Anthony F.

    2017-03-01

    Use of time delays in high-order-harmonic generation (HHG) driven by intense two-color, few-cycle pulses is investigated in order to determine means of optimizing HHG intensities and plateau cutoff energies. Based upon numerical solutions of the time-dependent Schrödinger equation for the H atom as well as analytical analyses, we show that introducing a time delay between the two-color, few-cycle pulses can result in an enhancement of the intensity of the HHG spectrum by an order of magnitude (or more) at the cost of a reduction in the HHG plateau cutoff energy. Results for both positive and negative time delays as well as various pulse carrier-envelope phases are investigated and discussed.

  11. Table-top two-color soft x-ray laser from Ni-like Mo plasma

    NASA Astrophysics Data System (ADS)

    Bleiner, Davide

    2017-05-01

    Two-color laser pulses in a laboratory setup are interesting for enabling a number of advanced spectroscopy techniques. The generation by means of Ni-like laser-produced plasmas is promising for scaling down the wavelength towards the soft X-ray. The occurrence of a two-color signal has been documented in the 1990s and a detailed atomic physics study interpreted the pulse as due to concomitant collisional pumping (color-1) and self-photoexcitation (color-2). If this framework is on the atomic physics basis valid, its kinetic and hydrodynamic quantitative aspects need to be better understood. In particular, experimental observations leave room for a number of open points. Here we propose am amplified Raman scattering (ARS) scheme, to explain the growth of color-2 and its sensitivity to the plasma irradiation scheme.

  12. Macroscopic scaling of high-order harmonics generated by two-color optimized waveforms in a hollow waveguide

    NASA Astrophysics Data System (ADS)

    Jin, Cheng; Hong, Kyung-Han; Lin, C. D.

    2017-07-01

    We present the macroscopic scaling of high harmonics generated by two-color laser pulses interacting with Ne gas in a hollow waveguide. We demonstrate that the divergence of harmonics is inversely proportional to the waveguide radius and harmonic yields are proportional to the square of the waveguide radius when the gas pressure and waveguide length are chosen to meet the phase-matching condition. We also show that harmonic yields are inversely proportional to the ionization level of the optimized two-color waveform with proper gas pressure if waveguide radius and length are fixed. These scaling relations would help experimentalists find phase-matching conditions to efficiently generate tabletop high-flux coherent soft x rays for applications.

  13. Two-color single-photon emission from InAs quantum dots: toward logic information management using quantum light.

    PubMed

    Rivas, David; Muñoz-Matutano, Guillermo; Canet-Ferrer, Josep; García-Calzada, Raúl; Trevisi, Giovanna; Seravalli, Luca; Frigeri, Paola; Martínez-Pastor, Juan P

    2014-02-12

    In this work, we propose the use of the Hanbury-Brown and Twiss interferometric technique and a switchable two-color excitation method for evaluating the exciton and noncorrelated electron-hole dynamics associated with single photon emission from indium arsenide (InAs) self-assembled quantum dots (QDs). Using a microstate master equation model we demonstrate that our single QDs are described by nonlinear exciton dynamics. The simultaneous detection of two-color, single photon emission from InAs QDs using these nonlinear dynamics was used to design a NOT AND logic transference function. This computational functionality combines the advantages of working with light/photons as input/output device parameters (all-optical system) and that of a nanodevice (QD size of ∼ 20 nm) while also providing high optical sensitivity (ultralow optical power operational requirements). These system features represent an important and interesting step toward the development of new prototypes for the incoming quantum information technologies.

  14. Parity-selective enhancement of field-free molecular orientation in an intense two-color laser field

    SciTech Connect

    Yun, Hyeok; Nam, Chang Hee; Kim, Hyung Taek; Kim, Chul Min; Lee, Jongmin

    2011-12-15

    We investigated the characteristics of molecular orientation induced by a nonresonant two-color femtosecond laser field. By analyzing the rotational dynamics of asymmetric linear molecules, we revealed that the critical parameter in characterizing the molecular orientation was the hyperpolarizability of molecules that selected the excitation paths of rotational states between parity-changing and parity-conserving transitions. Especially, in the case of molecules with small hyperpolarizability, a significant enhancement of orientation was achieved at the half-rotational period, instead of the full-rotational period. This deeper understanding of the hyperpolarizability-dependent characteristics of molecular orientation in a two-color scheme can provide an effective method to achieve significantly enhanced field-free orientation for various polar molecules.

  15. Matching-based fresh-slice method for generating two-color x-ray free-electron lasers

    NASA Astrophysics Data System (ADS)

    Qin, Weilun; Ding, Yuantao; Lutman, Alberto A.; Chao, Yu-Chiu

    2017-09-01

    Two-color high intensity x-ray free-electron lasers (FELs) provide powerful tools for probing ultrafast dynamic systems. A novel concept of realizing fresh-slice two-color lasing through slice-dependent transverse mismatch has been proposed by one of the authors [Y. Chao, SLAC Report No. SLAC-PUB-16935, 2016]. In this paper we present a feasible example following this concept based on the Linac Coherent Light Source parameters. Time-dependent mismatch along the bunch is generated by a passive dechirper module and controlled by downstream matching sections, enabling FEL lasing at different wavelength with a split undulator configuration. Simulations for soft x-ray FELs show that tens of gigawatts pulses with femtosecond duration can be generated.

  16. Improved treatment of the turning point in tunnel ionization of atoms in a low-frequency two color laser field

    NASA Astrophysics Data System (ADS)

    Petrović, V. M.; Miladinović, T. B.

    2016-12-01

    The tunneling photoionization rate for a two color (bichromatic) field consisting of coherent superposition of the fundamental laser field frequency ω and its second harmonic with frequency 2ω , was studied theoretically within the framework of the adiabatic Landau-Dykhne approach. Analytical expressions were derived for the case of fixed specified values of relative phase shift, \\varphi =0 between the harmonics of the incident bichromatic field.

  17. Orientation dependence of the ionization of CO and NO in an intense femtosecond two-color laser field

    SciTech Connect

    Li, H.; Ray, D.; De, S.; Cao, W.; Laurent, G.; Wang, Z.; Le, A. T.; Cocke, C. L.; Znakovskaya, I.; Kling, M. F.

    2011-10-15

    Two-color (800- and 400-nm) short (45-fs) linearly polarized pulses are used to ionize and dissociate CO and NO. The emission of C{sup q+}, N{sup q+}, and O{sup +} fragments indicates that the higher ionization rate occurs when the peak electric field points from C to O in CO and from N to O in NO. This preferred direction is in agreement with that predicted by Stark-corrected strong-field-approximation calculations.

  18. Two-color above-threshold ionization of atoms and ions in XUV Bessel beams and intense laser light

    NASA Astrophysics Data System (ADS)

    Seipt, D.; Müller, R. A.; Surzhykov, A.; Fritzsche, S.

    2016-11-01

    The two-color above-threshold ionization (ATI) of atoms and ions is investigated for a vortex Bessel beam in the presence of a strong near-infrared (NIR) light field. While the photoionization is caused by the photons from the weak but extreme ultraviolet (XUV) vortex Bessel beam, the energy and angular distribution of the photoelectrons and their sideband structure are affected by the plane-wave NIR field. We here explore the energy spectra and angular emission of the photoelectrons in such two-color fields as a function of the size and location of the target atoms with regard to the beam axis. In addition, analog to the circular dichroism in typical two-color ATI experiments with circularly polarized light, we define and discuss seven different dichroism signals for such vortex Bessel beams that arise from the various combinations of the orbital and spin angular momenta of the two light fields. For localized targets, it is found that these dichroism signals strongly depend on the size and position of the atoms relative to the beam. For macroscopically extended targets, in contrast, three of these dichroism signals tend to zero, while the other four just coincide with the standard circular dichroism, similar as for Bessel beams with a small opening angle. Detailed computations of the dichroism are performed and discussed for the 4 s valence-shell photoionization of Ca+ ions.

  19. Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform.

    PubMed

    Salehi, Reza; Tsoi, Stephen C M; Colazo, Marcos G; Ambrose, Divakar J; Robert, Claude; Dyck, Michael K

    2017-01-30

    Early embryonic loss is a large contributor to infertility in cattle. Moreover, bovine becomes an interesting model to study human preimplantation embryo development due to their similar developmental process. Although genetic factors are known to affect early embryonic development, the discovery of such factors has been a serious challenge. Microarray technology allows quantitative measurement and gene expression profiling of transcript levels on a genome-wide basis. One of the main decisions that have to be made when planning a microarray experiment is whether to use a one- or two-color approach. Two-color design increases technical replication, minimizes variability, improves sensitivity and accuracy as well as allows having loop designs, defining the common reference samples. Although microarray is a powerful biological tool, there are potential pitfalls that can attenuate its power. Hence, in this technical paper we demonstrate an optimized protocol for RNA extraction, amplification, labeling, hybridization of the labeled amplified RNA to the array, array scanning and data analysis using the two-color analysis strategy.

  20. Characterization of epoxyeicosatrienoic acid binding site in U937 membranes using a novel radiolabeled agonist, 20-125i-14,15-epoxyeicosa-8(Z)-enoic acid.

    PubMed

    Yang, Wenqi; Tuniki, Venugopal Raju; Anjaiah, Siddam; Falck, J R; Hillard, Cecilia J; Campbell, William B

    2008-03-01

    Epoxyeicosatrienoic acids (EETs) are important regulators of vascular tone and homeostasis. Whether they initiate signaling through membrane receptors is unclear. We developed 20-iodo-14,15-epoxyeicosa-8(Z)-enoic acid (20-I-14,15-EE8ZE), a radiolabeled EET agonist, to characterize EET binding to membranes of U937 cells. 20-I-14,15-EE8ZE stimulated cAMP production in U937 cells with similar potency, but it decreased efficacy compared with 11,12-EET. Maximum cAMP production increased 4.2-fold, with an EC(50) value of 9 muM. Like 14,15-EET, 20-I-14,15-EE8ZE relaxed bovine coronary arteries, with a similar EC(50) value. Both 20-I-14,15-EE8ZE agonist activities were blocked by the EET antagonist 14,15-epoxyeicosa-5(Z)enoic acid (14,15-EE5ZE). Specific 20-(125)I-14,15-EE8ZE binding to U937 membranes reached equilibrium within 10 min and remained unchanged for 30 min at 4 degrees C. The binding was saturable, reversible, and exhibited K(D) and B(max) values of 11.8 +/- 1.1 nM and 5.8 +/- 0.2 pmol/mg protein, respectively. Pretreatment of the membranes with guanosine 5'-O-(3-thio)triphosphate reduced the B(max) in a concentration-related manner. 20-(125)I-14,15-EE8ZE binding was inhibited by eicosanoids with potency order of 11,12-EET >14,15-EE5ZE approximately 14,15-EET > 15-hydroxyeicosatetraenoic acid > 14,15-EET-thiirane >14,15-dihydroxyeicosatrienoic acid. This order is in agreement with the efficacy and potency of cAMP production. In summary, 20-(125)I-14,15-EE8ZE is a radiolabeled EET agonist that is useful to study binding and metabolism. Using this radioligand, we have identified a specific high-affinity and high-abundance EET binding site in U937 cell membranes. This binding site could represent a specific EET receptor, which is probably a G protein-coupled receptor.

  1. Molecular characterization of the t(4;12)(q27~28;q14~15) chromosomal rearrangement in lipoma.

    PubMed

    Agostini, Antonio; Gorunova, Ludmila; Bjerkehagen, Bodil; Lobmaier, Ingvild; Heim, Sverre; Panagopoulos, Ioannis

    2016-09-01

    Lipomas are common benign soft tissue tumors whose genetic and cytogenetic features are well characterized. The karyotype is usually near- or pseudodiploid with characteristic structural chromosomal aberrations. The most common rearrangements target the high mobility group AT-hook 2 (HMGA2) gene in 12q14.3, with breakpoints occurring within or outside of the gene locus leading to deregulation of HMGA2. The most common fusion partner for HMGA2 in lipoma is lipoma-preferred partner (3q27), but also other genes frequently recombine with HMGA2. Furthermore, truncated HMGA2 transcripts are recurrently observed in lipomas. The present study describes 5 lipomas carrying the translocation t(4;12)(q27~28;q14~15) as the sole chromosomal anomaly, as well as 1 lipoma in which the three-way translocation t(1;4;12)(q21;q27~28;q14~15) was identified. Molecular analyses performed on 4 of these cases detected 4 truncated forms of HMGA2. In 3 tumors, the HMGA2 truncated transcripts included sequences originating from the chromosomal sub-band 4q28.1. Notably, in 2 of these cases, the fourth exon of HMGA2 was fused to transposable elements located in 4q28.1.

  2. Molecular characterization of the t(4;12)(q27~28;q14~15) chromosomal rearrangement in lipoma

    PubMed Central

    Agostini, Antonio; Gorunova, Ludmila; Bjerkehagen, Bodil; Lobmaier, Ingvild; Heim, Sverre; Panagopoulos, Ioannis

    2016-01-01

    Lipomas are common benign soft tissue tumors whose genetic and cytogenetic features are well characterized. The karyotype is usually near- or pseudodiploid with characteristic structural chromosomal aberrations. The most common rearrangements target the high mobility group AT-hook 2 (HMGA2) gene in 12q14.3, with breakpoints occurring within or outside of the gene locus leading to deregulation of HMGA2. The most common fusion partner for HMGA2 in lipoma is lipoma-preferred partner (3q27), but also other genes frequently recombine with HMGA2. Furthermore, truncated HMGA2 transcripts are recurrently observed in lipomas. The present study describes 5 lipomas carrying the translocation t(4;12)(q27~28;q14~15) as the sole chromosomal anomaly, as well as 1 lipoma in which the three-way translocation t(1;4;12)(q21;q27~28;q14~15) was identified. Molecular analyses performed on 4 of these cases detected 4 truncated forms of HMGA2. In 3 tumors, the HMGA2 truncated transcripts included sequences originating from the chromosomal sub-band 4q28.1. Notably, in 2 of these cases, the fourth exon of HMGA2 was fused to transposable elements located in 4q28.1. PMID:27588119

  3. Development of a novel two color tracer perfusion technique for the hydrodynamic study of aqueous outflow in bovine eyes

    PubMed Central

    Jing-yin, ZHU; Wen, YE; Hai-yan, GONG

    2010-01-01

    Background Elevation of intraocular pressure is usually associated with primary open angle glaucoma and caused by increased outflow resistance. A two-color fluorescent tracer technique was developed to investigate the hydrodynamics of aqueous humor outflow with changing intraocular pressure within the same eye, to better understand the relationship between outflow facility and effective filtration area. Methods Eighteen enucleated bovine eyes were first perfused at 30 mmHg with Dulbecco’s phosphate-buffered saline containing 5.5 mmol/L D-glucose. After a stable baseline facility, red fluorescent microspheres (0.5 μm, 0.002% v/v) were exchanged and perfused. Eyes in the one-color control group (n=6) were immediately perfused with fixative. In the experimental group (n=6), eyes were perfused with green tracer after intraocular pressure reduced to 7 mmHg, while in the two-color control group (n=6), eyes were perfused with green tracer with intraocular pressure remaining at 30 mmHg. All 12 eyes were then perfusion-fixed. Outflow facility was continuously recorded in all eyes. Confocal images were taken along the inner wall of the aqueous plexus and the percent of the effective filtration length (PEFL; length of inner wall exhibiting tracer labeling/total length of inner wall) was measured. The relationships between outflow facility and PEFL were analyzed statistically. Results No significant differences were found in baseline facilities (μl·min−1·mmHg−1) among the three groups (the experimental group: 0.93±0.12; the two-color control group: 0.90±0.19; the one-color control group: 0.98±0.13). In the experimental group, the outflow facility was significantly higher at 7 mmHg (4.29±1.01) than that at 30 mmHg (1.90±0.67, P <0.001), which corresponded to a significant increase in the PEFL at 7 mmHg (54.70±8.42) from that at 30 mmHg ((11.76±4.56)%, P <0.001). The PEFL labeled by red fluorescent microspheres in the experimental group ((11.76±4.56)%) showed

  4. Multi-terminal Two-color ZnCdSe/ZnCdMgSe Based Quantum-well Infrared Photodetector

    NASA Astrophysics Data System (ADS)

    Kaya, Yasin; Ravikumar, Arvind; Chen, Guopeng; Tamargo, Maria C.; Shen, Aidong; Gmachl, Claire

    Target recognition and identification applications benefits from two-color infrared (IR) detectors in the mid and long-wavelength IR regions. Currently, InGaAs/AlGaAs and GaAs/AlGaAs multiple quantum wells (QWs) grown on GaAs substrate are the most commonly used two-color QW IR photodetectors (QWIPs). However, the lattice-mismatch and the buildup of strain limit the number of QWs that can be grown, in turn increasing the dark current noise, and limiting the device detectivity.In this work, we report on two-color QWIPs based on the large conduction band offset (~1.12ev) ZnCdSe/ZnCdMgSe material system lattice matched to InP. QWIPs were designed based on a bound to quasi-bound transition, centered at 4 μm and 7 μm and each QW is repeated 50 times to eliminate the high dark current and a contact layer is inserted between the two stacks of QWs for independent electrical contacts. Wafers are processed into two step rectangular mesas by lithography and wet etching. Experiments showed absorption spectra centered at 4.9 μm and 7.6 μm at 80 K and the full width at half maximums were Δλ / λ = 21 % and Δλ / λ = 23 % , respectively. Current work studies the Johnson and the background noise limited detectivities of these QWIPs. Current address: School of Earth, Energy and Environmental Sciences, Stanford, CA 94305, USA.

  5. Analytic description of high-order harmonic generation by atoms in a two-color laser field

    SciTech Connect

    Frolov, M. V.; Manakov, N. L.; Silaev, A. A.; Vvedenskii, N. V.

    2010-06-15

    A closed-form analytic formula describing high-order harmonic generation (HHG) in a two-color field of frequencies {omega} and 2{omega} is derived quantum mechanically in the low-frequency (tunneling) limit for an electron bound by a short-range potential and generalized to the case of an active electron in a neutral atom. The HHG rates are presented as a product of an electron wave packet describing the ionization of an active electron and its propagation in a laser field up to the recombination event and an atom-specific cross section of the electron's photorecombination. In contrast to the case of a monochromatic laser pulse [Frolov et al., Phys. Rev. Lett. 102, 243901 (2009)], the two-color wave packet involves the interference of two terms (involving the Airy function) that describe the emission of harmonics during the first and second half-cycles of the fundamental laser cycle and give rise to the two-plateau structures in the HHG spectra. For the case of the H atom, we show that our analytic results are in good agreement with those obtained from a numerical solution of the three-dimensional time-dependent Schroedinger equation. The factorization formula is used for describing the dependence of HHG rates for inert gases on the relative phase and intensities of the {omega} and 2{omega} components of a laser field. It is shown that atomic structure (including electron correlation) effects can modify substantially the two-color HHG spectra of inert gases.

  6. Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms.

    PubMed

    Neumann, Daniel; Bückers, Johanna; Kastrup, Lars; Hell, Stefan W; Jakobs, Stefan

    2010-03-05

    The voltage-dependent anion channel (VDAC, also known as mitochondrial porin) is the major transport channel mediating the transport of metabolites, including ATP, across the mitochondrial outer membrane. Biochemical data demonstrate the binding of the cytosolic protein hexokinase-I to VDAC, facilitating the direct access of hexokinase-I to the transported ATP. In human cells, three hVDAC isoforms have been identified. However, little is known on the distribution of these isoforms within the outer membrane of mitochondria and to what extent they colocalize with hexokinase-I. In this study we show that whereas hVDAC1 and hVDAC2 are localized predominantly within the same distinct domains in the outer membrane, hVDAC3 is mostly uniformly distributed over the surface of the mitochondrion. We used two-color stimulated emission depletion (STED) microscopy enabling a lateral resolution of ~40 nm to determine the detailed sub-mitochondrial distribution of the three hVDAC isoforms and hexokinase-I. Individual hVDAC and hexokinase-I clusters could thus be resolved which were concealed in the confocal images. Quantitative colocalization analysis of two-color STED images demonstrates that within the attained resolution, hexokinase-I and hVDAC3 exhibit a higher degree of colocalization than hexokinase-I with either hVDAC1 or hVDAC2. Furthermore, a substantial fraction of the mitochondria-bound hexokinase-I pool does not colocalize with any of the three hVDAC isoforms, suggesting a more complex interplay of these proteins than previously anticipated. This study demonstrates that two-color STED microscopy in conjunction with quantitative colocalization analysis is a powerful tool to study the complex distribution of membrane proteins in organelles such as mitochondria.PACS: 87.16.Tb, 87.85.Rs.

  7. Coherent control of D2/H2 dissociative ionization by a mid-infrared two-color laser field

    NASA Astrophysics Data System (ADS)

    Wanie, Vincent; Ibrahim, Heide; Beaulieu, Samuel; Thiré, Nicolas; Schmidt, Bruno E.; Deng, Yunpei; Alnaser, Ali S.; Litvinyuk, Igor V.; Tong, Xiao-Min; Légaré, François

    2016-01-01

    Steering the electrons during an ultrafast photo-induced process in a molecule influences the chemical behavior of the system, opening the door to the control of photochemical reactions and photobiological processes. Electrons can be efficiently localized using a strong laser field with a well-designed temporal shape of the electric component. Consequently, many experiments have been performed with laser sources in the near-infrared region (800 nm) in the interest of studying and enhancing the electron localization. However, due to its limited accessibility, the mid-infrared (MIR) range has barely been investigated, although it allows to efficiently control small molecules and even more complex systems. To push further the manipulation of basic chemical mechanisms, we used a MIR two-color (1800 and 900 nm) laser field to ionize H2 and D2 molecules and to steer the remaining electron during the photo-induced dissociation. The study of this prototype reaction led to the simultaneous control of four fragmentation channels. The results are well reproduced by a theoretical model solving the time-dependent Schrödinger equation for the molecular ion, identifying the involved dissociation mechanisms. By varying the relative phase between the two colors, asymmetries (i.e., electron localization selectivity) of up to 65% were obtained, corresponding to enhanced or equivalent levels of control compared to previous experiments. Experimentally easier to implement, the use of a two-color laser field leads to a better electron localization than carrier-envelope phase stabilized pulses and applying the technique in the MIR range reveals more dissociation channels than at 800 nm.

  8. Quantitation of Viral DNA by Real-Time PCR Applying Duplex Amplification, Internal Standardization, and Two-Color Fluorescence Detection

    PubMed Central

    Gruber, Franz; Falkner, Falko G.; Dorner, Friedrich; Hämmerle, Thomas

    2001-01-01

    A real-time PCR method was developed to quantitate viral DNA that includes duplex amplification, internal standardization, and two-color fluorescence detection without the need to generate an external standardization curve. Applied to human parvovirus B19 DNA, the linear range was from 102 to at least 5 × 106 copies per ml of sample. The coefficient of variation was 0.29 using a run control of 2,876 copies per ml. The method reduces the risk of false-negative results, yields high precision, and is applicable for other DNA targets. PMID:11375203

  9. Laser-sub-cycle two-dimensional electron-momentum mapping using orthogonal two-color fields

    NASA Astrophysics Data System (ADS)

    Zhang, Li; Xie, Xinhua; Roither, Stefan; Kartashov, Daniil; Wang, YanLan; Wang, ChuanLiang; Schöffler, Markus; Shafir, Dror; Corkum, Paul B.; Baltuška, Andrius; Ivanov, Igor; Kheifets, Anatoli; Liu, XiaoJun; Staudte, André; Kitzler, Markus

    2014-12-01

    We study laser-sub-cycle control over electron trajectories concomitantly in space and time using orthogonally polarized two-color laser fields. We compare experimental photoelectron spectra of neon recorded by coincidence momentum imaging with photoelectron spectra obtained by semiclassical and numerical solutions of the time-dependent Schrödinger equation. We find that a resolution of a quarter optical cycle in the photoelectron trajectories can be achieved. It is shown that depending on their sub-cycle birth time the trajectories of photoelectrons are affected differently by the ion's Coulomb field.

  10. A Two Colorable Fourth Order Compact Difference Scheme and Parallel Iterative Solution of the 3D Convection Diffusion Equation

    NASA Technical Reports Server (NTRS)

    Zhang, Jun; Ge, Lixin; Kouatchou, Jules

    2000-01-01

    A new fourth order compact difference scheme for the three dimensional convection diffusion equation with variable coefficients is presented. The novelty of this new difference scheme is that it Only requires 15 grid points and that it can be decoupled with two colors. The entire computational grid can be updated in two parallel subsweeps with the Gauss-Seidel type iterative method. This is compared with the known 19 point fourth order compact differenCe scheme which requires four colors to decouple the computational grid. Numerical results, with multigrid methods implemented on a shared memory parallel computer, are presented to compare the 15 point and the 19 point fourth order compact schemes.

  11. Revealing the Sub-Barrier Phase using a Spatiotemporal Interferometer with Orthogonal Two-Color Laser Fields of Comparable Intensity

    NASA Astrophysics Data System (ADS)

    Han, Meng; Ge, Peipei; Shao, Yun; Liu, Ming-Ming; Deng, Yongkai; Wu, Chengyin; Gong, Qihuang; Liu, Yunquan

    2017-08-01

    We measure photoelectron momentum distributions of Ar atoms in orthogonally polarized two-color laser fields with comparable intensities. The synthesized laser field is used to manipulate the oscillating tunneling barrier and the subsequent motion of electrons onto two spatial dimensions. The subcycle structures associated with the temporal double-slit interference are spatially separated and enhanced. We use such a spatiotemporal interferometer to reveal sub-barrier phase of strong-field tunneling ionization. This study shows that the tunneling process transfers the initial phase onto momentum distribution. Our work has the implication that the sub-barrier phase plays an indispensable role in photoelectron interference processes.

  12. Two color laser fields for studying the Cooper minimum with phase-matched high-order harmonic generation

    SciTech Connect

    Ba Dinh, Khuong Vu Le, Hoang; Hannaford, Peter; Van Dao, Lap

    2014-05-28

    We experimentally study the observation of the Cooper minimum in a semi-infinite argon-filled gas cell using two-color laser fields at wavelengths of 1400 nm and 800 nm. The experimental results show that the additional 800 nm field can change the macroscopic phase-matching condition through change of the atomic dipole phase associated with the electron in the continuum state and that this approach can be used to control the appearance of the Cooper minimum in the high-order harmonic spectrum in order to study the electronic structure of atoms and molecules.

  13. Two-dimensional bi-periodic grating coupled one- and two-color quantum well infrared photodetectors

    SciTech Connect

    Tsai, K.L.; Lee, C.P.; Tsang, J.S.; Chen, H.R.; Chang, K.H.

    1995-02-01

    Multiple quantum well infrared photodetectors (QWIP`s) with two-dimensional bi-periodic grating couplers have been demonstrated. For one-color QWIP`s, the response linewidths of such QWIP`s are found to be much wider than those of conventional grating coupled QWIP`s. The bi-periodic gratings have also been applied to two-color QWIP`s to separately optimize the absorption at two wavelength bands. The large difference in responsivities at the two wavelength bands for devices with single period gratings can be greatly reduced with the use of bi-periodic gratings.

  14. Observations of strain accumulation across the San Andreas fault near Palmdale, California, with a two-color geodimeter

    USGS Publications Warehouse

    Langbein, J.O.; Linker, M.F.; McGarr, A.; Slater, L.E.

    1982-01-01

    Two-color laser ranging measurements during a 15-month period over a geodetic network spanning the San Andreas fault near Palmdale, California, indicate that the crust expands and contracts aseismically in episodes as short as 2 weeks. Shear strain parallel to the fault has accumulated monotonically since November 1980, but at a variable rate. Improvements in measurement precision and temporal resolution over those of previous geodetic studies near Palmdale have resulted in the definition of a time history of crustal deformation that is much more complex than formerly realized. Copyright ?? 1982 AAAS.

  15. Control of reaction efficiency by two-color two-pulse excitation: Photoisomerization of indocyanine green in condensed phase

    NASA Astrophysics Data System (ADS)

    Fuyuki, Masanori; Furuta, Koichi; Wada, Akihide

    2011-07-01

    In cyanine dye, photoisomerization paths involving multiphoton processes were found by two-color two-pulse correlation of ultrafast transient absorption spectroscopy. The reaction efficiency depended on the interval between two pump pulses, UV and NIR pulses, as well as the fluence of the NIR pulse, but was little dependent on that of the UV pulse. These results indicate that the new photoisomerization paths involve the NIR multiphoton process and/or the UV one-photon process. The difference in two-pulse correlation between positive and negative time regions revealed a new reaction path that had the highest branching ratio of cis isomer to other leuco forms.

  16. Theoretical investigation of a novel high density cage compound 4,8,11,14,15-pentanitro-2,6,9,13-tetraoxa-4,8,11,14,15-pentaazaheptacyclo[5.5.1.1(3,11).1(5,9)] pentadecane.

    PubMed

    Lin, He; Zhu, Shun-guan; Zhang, Lin; Peng, Xin-hua; Chen, Peng-yuan; Li, Hong-zhen

    2013-03-01

    A novel polynitro cage compound 4,8,11,14,15-pentanitro-2,6,9,13-tetraoxa-4,8,11,14,15-pentaazaheptacyclo [5.5.1.1(3,11).1(5,9)]pentadecane(PNTOPAHP) has been designed and investigated at the DFT-B3LYP/6-31(d) level. Properties, such as electronic structure, IR spectrum, heat of formation, thermodynamic properties and crystal structure have been predicted. This compound is most likely to crystallize in C2/c space group, and the corresponding cell parameters are Z = 8, a = 29.78 Å, b = 6.42 Å, c = 32.69 Å, α = 90.00°, β = 151.05°, γ = 90.00° and ρ = 1.94 g/cm(3). In addition, the detonation velocity and pressure have also been calculated by the empirical Kamlet-Jacobs equation. As a result, the detonation velocity and pressure of this compound are 9.82 km/s, 44.67 GPa, respectively, a little higher than those of 4,10-dinitro-2,6,8,12-tetraoxa-4,10-diazaisowurtzitane(TEX, 9.28 km/s, 40.72 GPa). This compound has a comparable chemical stability to TEX, based on the N-NO(2) trigger bond length analysis. The bond dissociation energy ranges from 153.09 kJ mol(-1) to 186.04 kJ mol(-1), which indicates that this compound meets the thermal stability requirement as an exploitable HEDM.

  17. Terahertz radiation driven by two-color laser pulses at near-relativistic intensities: Competition between photoionization and wakefield effects

    PubMed Central

    González de Alaiza Martínez, P.; Davoine, X.; Debayle, A.; Gremillet, L.; Bergé, L.

    2016-01-01

    We numerically investigate terahertz (THz) pulse generation by linearly-polarized, two-color femtosecond laser pulses in highly-ionized argon. Major processes consist of tunneling photoionization and ponderomotive forces associated with transverse and longitudinal field excitations. By means of two-dimensional particle-in-cell (PIC) simulations, we reveal the importance of photocurrent mechanisms besides transverse and longitudinal plasma waves for laser intensities >1015 W/cm2. We demonstrate the following. (i) With two-color pulses, photoionization prevails in the generation of GV/m THz fields up to 1017 W/cm2 laser intensities and suddenly loses efficiency near the relativistic threshold, as the outermost electron shell of ionized Ar atoms has been fully depleted. (ii) PIC results can be explained by a one-dimensional Maxwell-fluid model and its semi-analytical solutions, offering the first unified description of the main THz sources created in plasmas. (iii) The THz power emitted outside the plasma channel mostly originates from the transverse currents. PMID:27255689

  18. High-order harmonic generation of N2 molecule in two-color circularly polarized laser fields

    NASA Astrophysics Data System (ADS)

    Hui, Du; Jun, Zhang; Shuai, Ben; Hui-Ying, Zhong; Tong-Tong, Xu; Jing, Guo; Xue-Shen, Liu

    2016-04-01

    The generation of high-order harmonics and the attosecond pulse of the N2 molecule in two-color circularly polarized laser fields are investigated by the strong-field Lewenstein model. We show that the plateau of spectra is dramatically extended and a continuous harmonic spectrum with the bandwidth of 113 eV is obtained. When a static field is added to the x direction, the quantum path control is realized and a supercontinuum spectrum can be obtained, which is beneficial to obtain a shorter attosecond pulse. The underlying physical mechanism is well explained by the time-frequency analysis and the semi-classical three-step model with a finite initial transverse velocity. By superposing several orders of harmonics in the combination of two-color circularly polarized laser fields and a static field, an isolated attosecond pulse with a duration of 30 as can be generated. Project supported by the National Natural Science Foundation of China (Grant Nos. 61575077, 11271158, and 11574117).

  19. Terahertz radiation driven by two-color laser pulses at near-relativistic intensities: Competition between photoionization and wakefield effects

    NASA Astrophysics Data System (ADS)

    González de Alaiza Martínez, P.; Davoine, X.; Debayle, A.; Gremillet, L.; Bergé, L.

    2016-06-01

    We numerically investigate terahertz (THz) pulse generation by linearly-polarized, two-color femtosecond laser pulses in highly-ionized argon. Major processes consist of tunneling photoionization and ponderomotive forces associated with transverse and longitudinal field excitations. By means of two-dimensional particle-in-cell (PIC) simulations, we reveal the importance of photocurrent mechanisms besides transverse and longitudinal plasma waves for laser intensities >1015 W/cm2. We demonstrate the following. (i) With two-color pulses, photoionization prevails in the generation of GV/m THz fields up to 1017 W/cm2 laser intensities and suddenly loses efficiency near the relativistic threshold, as the outermost electron shell of ionized Ar atoms has been fully depleted. (ii) PIC results can be explained by a one-dimensional Maxwell-fluid model and its semi-analytical solutions, offering the first unified description of the main THz sources created in plasmas. (iii) The THz power emitted outside the plasma channel mostly originates from the transverse currents.

  20. One Micron Laser Technology Advancements at GSFC

    NASA Technical Reports Server (NTRS)

    Heaps, William S.

    2010-01-01

    This slide presentation reviews the advancements made in one micron laser technology at Goddard Space Flight Center. It includes information about risk factors that are being addressed by GSFC, and overviews of the various programs that GSFC is currently managing that are using 1 micron laser technology.

  1. High Energy 2-Micron Laser Developments

    NASA Technical Reports Server (NTRS)

    Yu, Jirong; Trieu, Bo C.; Petros, Mulugeta; Bai, Yingxin; Petzar, Paul J.; Koch, Grady J.; Singh, Upendra N.; Kavaya, Michael J.

    2007-01-01

    A master oscillator power amplifier, high energy Q-switched 2-micron laser system has been recently demonstrated. The laser and amplifiers are all designed in side-pumped rod configuration, pumped by back-cooled conductive packaged GaAlAs diode laser arrays. This 2-micron laser system provides nearly transform limited beam quality.

  2. A Two-Color Fourier Transform Mm-Wave Spectrometer for Gas Analysis Operating from 260-295 GHZ

    NASA Astrophysics Data System (ADS)

    Steber, Amanda L.; Harris, Brent J.; Lehmann, Kevin K.; Pate, Brooks H.

    2013-06-01

    We have designed a two-color mm-wave spectrometer for Fourier transform mm-wave spectroscopy that uses consumer level components for the tunable synthesizers, digital control of the pulse modulators, and digitization of the coherent free induction decay (FID). The excitation pulses are generated using an x24 active multiplier chain (AMC) that produces a peak power of 30 mW. The microwave input to the AMC is generated in a frequency up conversion circuit that accepts a microwave input frequency from about 2-4 GHz. This circuit also generates the input to the mm-wave subhamonic mixer that creates the local oscillator from a separate 2-4 GHz microwave input. Excitation pulses at two independently tunable frequencies are generated using a dual-channel source based on a low-cost, wideband synthesizer integrated circuit (Valon Technology Model 5008). The outputs of the synthesizer are pulse modulated using a PIN diode switch that is driven using the arbitrary waveform generator (AWG) output of a USB-controlled high-speed digitizer / arbitrary waveform generator combination unit (Tie Pie HS-5 530 XM). The two pulses are combined using a Wilkinson power divider before input to the up conversion circuit. The FID frequency is down converted in a two-stage mixing process to 65 MHz. The two LO frequencies used in the receiver are provided by a second Valon 5008. The FID is digitized at 200 MSamples/s using the 12-bit Tie Pie digitizer. The digital oscilloscope (and its AWG channel) and the two synthesizers use a 10 MHz reference signal from a Rubidium clock to permit time-domain signal averaging. A key feature of the digital oscilloscope is its deep memory of 32 Mpts (complemented by the 64 Mpt memory in the 240 MS/s AWG). This makes it possible to perform several one- and two-color coherent measurements, including pulse echoes and double-resonance spectroscopy, in a single "readout" experiment to speed the analysis of mm-wave rotational spectra. The spectrometer sensitivity

  3. Status of two-color and large format HgCdTe FPA technology at Raytheon Vision Systems

    NASA Astrophysics Data System (ADS)

    Smith, E. P. G.; Bornfreund, R. E.; Kasai, I.; Pham, L. T.; Patten, E. A.; Peterson, J. M.; Roth, J. A.; Nosho, B. Z.; De Lyon, T. J.; Jensen, J. E.; Bangs, J. W.; Johnson, S. M.; Radford, W. A.

    2006-02-01

    Raytheon Vision Systems (RVS) is developing two-color and large format single color FPAs fabricated from molecular beam epitaxy (MBE) grown HgCdTe triple layer heterojunction (TLHJ) wafers on CdZnTe substrates and double layer heterojunction (DLHJ) wafers on Si substrates, respectively. MBE material growth development has resulted in scaling TLHJ growth on CdZnTe substrates from 10cm2 to 50cm2, long-wavelength infrared (LWIR) DLHJ growth on 4-inch Si substrates and the first demonstration of mid-wavelength infrared (MWIR) DLHJ growth on 6-inch Si substrates with low defect density (<1000cm -2) and excellent uniformity (composition<0.1%, cut-off wavelength Δcenter-edge<0.1μm). Advanced FPA fabrication techniques such as inductively coupled plasma (ICP) etching are being used to achieve high aspect ratio mesa delineation of individual detector elements with benefits to detector performance. Recent two-color detectors with MWIR and LWIR cut-off wavelengths of 5.5μm and 10.5μm, respectively, exhibit significant improvement in 78K LW performance with >70% quantum efficiency, diffusion limited reverse bias dark currents below 300pA and RA products (zero field-of-view, +150mV bias) in excess of 1×103 Ωcm2. Two-color 20μm unit-cell 1280×720 MWIR/LWIR FPAs with pixel response operability approaching 99% have been produced and high quality simultaneous imaging of the spectral bands has been achieved by mating the FPA to a readout integrated circuit (ROIC) with Time Division Multiplexed Integration (TDMI). Large format mega pixel 20μm unit-cell 2048×2048 and 25μm unit-cell 2560×512 FPAs have been demonstrated using DLHJ HgCdTe growth on Si substrates in the short wavelength infrared (SWIR) and MWIR spectral range. Recent imaging of 30μm unit-cell 256×256 LWIR FPAs with 10.0-10.7μm 78K cut-off wavelength and pixel response operability as high as 99.7% show the potential for extending HgCdTe/Si technology to LWIR wavelengths.

  4. Method for sampling sub-micron particles

    DOEpatents

    Gay, Don D.; McMillan, William G.

    1985-01-01

    Apparatus and method steps for collecting sub-micron sized particles include a collection chamber and cryogenic cooling. The cooling is accomplished by coil tubing carrying nitrogen in liquid form, with the liquid nitrogen changing to the gas phase before exiting from the collection chamber in the tubing. Standard filters are used to filter out particles of diameter greater than or equal to 0.3 microns; however the present invention is used to trap particles of less than 0.3 micron in diameter. A blower draws air to said collection chamber through a filter which filters particles with diameters greater than or equal to 0.3 micron. The air is then cryogenically cooled so that moisture and sub-micron sized particles in the air condense into ice on the coil. The coil is then heated so that the ice melts, and the liquid is then drawn off and passed through a Buchner funnel where the liquid is passed through a Nuclepore membrane. A vacuum draws the liquid through the Nuclepore membrane, with the Nuclepore membrane trapping sub-micron sized particles therein. The Nuclepore membrane is then covered on its top and bottom surfaces with sheets of Mylar.RTM. and the assembly is then crushed into a pellet. This effectively traps the sub-micron sized particles for later analysis.

  5. Sub-micron particle sampler apparatus

    DOEpatents

    Gay, Don D.; McMillan, William G.

    1987-01-01

    Apparatus and method steps for collecting sub-micron sized particles include a collection chamber and cryogenic cooling. The cooling is accomplished by coil tubing carrying nitrogen in liquid form, with the liquid nitrogen changing to the gas phase before exiting from the collection chamber in the tubing. Standard filters are used to filter out particles of diameter greater than or equal to 0.3 microns; however the present invention is used to trap particles of less than 0.3 micron in diameter. A blower draws air to said collection chamber through a filter which filters particles with diameters greater than or equal to 0.3 micron. The air is then cryogenically cooled so that moisture and sub-micron sized particles in the air condense into ice on the coil. The coil is then heated so that the ice melts, and the liquid is then drawn off and passed through a Buchner funnel where the liquid is passed through a Nuclepore membrane. A vacuum draws the liquid through the Nuclepore membrane, with the Nuclepore membrane trapping sub-micron sized particles therein. The Nuclepore membrane is then covered on its top and bottom surfaces with sheets of Mylar.RTM. and the assembly is then crushed into a pellet. This effectively traps the sub-micron sized particles for later analysis.

  6. Systematic studies of two-color pump-induced high-order harmonic generation in plasma plumes

    SciTech Connect

    Ganeev, R. A.; Singhal, H.; Naik, P. A.; Chakera, J. A.; Vora, H. S.; Khan, R. A.; Gupta, P. D.

    2010-11-15

    High-order harmonic generation (HHG) has been studied in various laser-produced plasma plumes using a two-color orthogonally polarized beam with a 12:1 energy ratio between the fundamental and second-harmonic (SH) components. The influence of the relative phase between the fundamental and SH waves on the HHG efficiency has been investigated. Odd and even harmonic generation in plasma plumes containing nanoparticles, fullerenes, carbon nanotubes, and other samples was optimized. The effect of the variation in the SH intensity on the HHG conversion efficiency in carbon aerogel and silver plasma plumes has also been studied. It is shown that by increasing the SH intensity, one can generate only even harmonics by suppressing the odd harmonics.

  7. Influences of different gases on the terahertz radiation based on the application of two-color laser pulses

    SciTech Connect

    Moradi, S.; Ganjovi, A.; Shojaei, F.; Saeed, M.

    2015-10-15

    In this work, using a two-dimensional Particle In Cell-Monte Carlo Collision simulation method, a comparative study is performed on the influences of different types of atomic and molecular gases at various background gas pressures on the generation of broadband and intense Terahertz (THz) radiation via the application of two-color laser pulses. These two modes are focused into Argon (Ar), Xenon (Xe), Nitrogen (N{sub 2}), Oxygen (O{sub 2}), and air as the background gaseous media and the plasma channel is created. It is observed that the THz radiation emission dramatically changes due to the propagation effects. A wider THz pulse is emitted from the formed plasma channel at the higher gas pressures. The significant effects of the propagation features of the emitted THz pulse on its energy at the longer lengths of the plasma channel are observed.

  8. Simultaneous measurement of temperature and velocity of air flow over 1000°C using two color phosphor thermometry

    NASA Astrophysics Data System (ADS)

    Fukuta, Masatoshi; Someya, Satoshi; Munakata, Tetsuo; LCS Team

    2016-11-01

    Thermal barrier coatings were applied to the gas turbines and the internal combustion engines for the high thermal efficiency. The evaluation and the improvement of coatings require to measure transient gaseous flow near the wall with coatings. An aim of this study is to combine a two color phosphor thermometry with the PIV to measure simultaneously temperature and velocity of the gas over 1000°C. The temperature and velocity distribution of an impinging jet of high temperature air was simultaneously visualized in experiments. The temperature was estimated from an intensity ratio of luminescent in different ranges of wavelength, 500 600 nm and 400 480 nm. Uncertainty of measured temperature was less than 10°C. Temperatures measured by the developed method and by thermocouples were agreed well. The measured velocity by the PIV with phosphor particles were also agreed well with the velocity measured by a Laser Doppler Velocimeter.

  9. Theoretical exploration of control factors for the high-order harmonic generation (HHG) spectrum in two-color field.

    PubMed

    Huang, Xinting; Yang, Dapeng; Yao, Li

    2014-09-15

    In this work, the laser-parameter effects on the high-order harmonic generation (HHG) spectrum and attosecond trains by mixing two-color laser field, a visible light field of 800 nm and a mid-infrared (mid-IR) laser pulses of 2400 nm, are theoretically demonstrated for the first time. Different schemes are applied to discuss the function of intensity, carrier-envelope phase (CEP) and pulse duration on the generation of an isolated attosecond pulse. As a consequence, an isolated 16as pulse is obtained by Fourier transforming an ultrabroad XUV continuum of 208 eV with the fundamental field of duration of 6 fs, 9×10(14)W/cm2 of intensity, the duration of 12 fs, the CEPs of the two driving pulses of -π and the relative strength ratio √R=0.2.

  10. Scaling behavior of ultrafast two-color terahertz generation in plasma gas targets: energy and pressure dependence.

    PubMed

    Rodriguez, George; Dakovski, Georgi L

    2010-07-05

    Ultrafast terahertz emission from two-color generated laser plasma gas targets is studied using air and the noble gases (neon, argon, krypton, and xenon) as the generation media. Terahertz output pulse energy and power spectra are measured as function of gas species, gas pressure, and input pulse energy up to 6 mJ per pulse using a 40-fs 1-kHz Ti:sapphire laser system as the drive source. Terahertz pulse energies approaching 1 microJ per pulse with spectral content out to 40 THz and pulse duration of 35 fs is reported. A simple one dimensional transient photocurrent ionization model is used to calculate the spectra showing good agreement with experiments.

  11. Two color multichannel heterodyne interferometer set up for high spatial resolution electron density profile measurements in TJ-II

    SciTech Connect

    Pedreira, P.; Criado, A. R.; Acedo, P.; Esteban, L.; Sanchez, M.; Sanchez, J.

    2010-10-15

    A high spatial resolution two color [CO{sub 2}, {lambda}=10.6 {mu}m/Nd:YAG (Nd:YAG denotes neodymium-doped yttrium aluminum garnet), and {lambda}=1.064 {mu}m] expanded-beam multichannel heterodyne interferometer has been installed on the TJ-II stellarator. Careful design of the optical system has allowed complete control on the evolution of both Gaussian beams along the interferometer, as well as the evaluation and optimization of the spatial resolution to be expected in the measurements. Five CO{sub 2} (measurement) channels and three Nd:YAG (vibration compensation) channels have been used to illuminate the plasma with a probe beam of 100 mm size. An optimum interpolation method has been applied to recover both interferometric phasefronts prior to mechanical vibration subtraction. The first results of the installed diagnostic are presented in this paper.

  12. Interaction of toluene with two-color asymmetric laser fields: Controlling the directional emission of molecular hydrogen fragments

    SciTech Connect

    Kaziannis, S.; Kotsina, N.; Kosmidis, C.

    2014-09-14

    The interaction of toluene with strong asymmetric two-color laser irradiation of 40 fs duration is studied by means of Time of flight mass spectrometry. Highly energetic H{sub 2}{sup +} and H{sub 3}{sup +} fragment ions are produced through an isomerization process taking place within transient multiply charged parent ions. Comparative study of deuterium labeled toluene isotopes enables the discrimination between molecular hydrogen fragments formed exclusively within the CH{sub 3}- part from those that require hydrogen atom exchange between the former and the phenyl moiety. It is demonstrated that by manipulating the relative phase of the ω/2ω field components the selective ionization of oriented toluene molecules can be used as a tool to control the directional emission of the H{sub 2}{sup +}, H{sub 3}{sup +} species.

  13. Two-color light-emitting diodes with polarization-sensitive high extraction efficiency based on graphene

    NASA Astrophysics Data System (ADS)

    H, Sattarian; S, Shojaei; E, Darabi

    2016-05-01

    In the present study, graphene photonic crystals are employed to enhance the light extraction efficiency (LEE) of two-color, red and blue, light-emitting diode (LED). The transmission characteristics of one-dimensional (1D) Fibonacci graphene photonic crystal LED (FGPC-LED) are investigated by using the transfer matrix method and the scaling study is presented. We analyzed the influence of period, thickness, and permittivity in the structure to enhance the LEE. The transmission spectrum of 1D FGPC has been optimized in detail. In addition, the effects of the angle of incidence and the state of polarization are investigated. As the main result, we found the optimum values of relevant parameters to enhance the extraction of red and blue light from an LED as well as provide perfect omnidirectional and high peak transmission filters for the TE and TM modes.

  14. Remote generation of high-energy terahertz pulses from two-color femtosecond laser filamentation in air

    SciTech Connect

    Wang, T.-J.; Daigle, J.-F.; Yuan, S.; Chin, S. L.; Theberge, F.; Chateauneuf, M.; Dubois, J.; Roy, G.; Zeng, H.

    2011-05-15

    We experimentally investigated the dynamic behavior of remote terahertz (THz) generation from two-color femtosecond laser-induced filamentation in air. A record-high THz pulse energy of 570 nJ at frequency below 5.5 THz was measured by optimizing the pump parameters at a controllable remote distance of 16 m, while super-broadband THz (<300 THz) pulse energy was up to 2.8 {mu}J. A further energy-scaling possibility was proposed. By analyzing simultaneously the fluorescence from both neutral N{sub 2} and N{sub 2}{sup +} in the filament, we found that the enhancement of THz radiation was due principally to guiding of the weak second-harmonic pulse inside the filament of the first strong fundamental pulse.

  15. Ultrafast molecular photoionization by two-color orthogonally polarized ultraviolet laser pulses: Effects of relative pulse phases

    NASA Astrophysics Data System (ADS)

    Yuan, Kai-Jun; Chelkowski, Szczepan; Bandrauk, André D.

    2017-09-01

    We present molecular photoionization by two-color 2ω1 =ω2 orthogonally polarized ultraviolet laser pulses. Simulations are performed on aligned H+ by numerically solving time-dependent Schrödinger equations. Two ionization processes with one ω2 photon interfering with two ω1 photon absorption are studied at different molecular alignments. Molecular frame photoelectron momentum and angular distributions exhibit asymmetries which are functions of the relative pulse phase. For resonant excitation processes by the ω1 pulse, symmetric distributions are obtained. An attosecond ionization model is adopted to describe the ultrafast ionization dynamics. The dependence of the ionization asymmetry on the molecular alignment allows to further monitor interference effects on orbital symmetry.

  16. Two-color interference effect involving three-photon atomic excitation and four-wave mixing in crossed laser beams

    SciTech Connect

    Peet, V.

    2007-09-15

    Through multiphoton ionization measurements, the polarization effects in destructive quantum interference under three-photon resonant excitation have been studied. Recent observations [V. Peet, Phys. Rev. A 74, 033406 (2006)] have indicated that contrary to the well-known pattern of a total suppression of resonance excitation, the destructive interference becomes incomplete if three-photon transition is driven by crossed beams with orthogonal polarization planes. These observations have been tested for a more general case of two-color excitation and very similar polarization-dependent anomalies in the interference character have been registered. It has been shown that the destructive interference is modified and the resonance excitation does occur if two crossed laser beams have opposite circular polarizations. The pressure-induced evolution of the uncanceled ionization peaks has the ratio of blue shift to width close to 0.5 exactly as it is known for resonance ionization peaks registered under excitation by counterpropagating laser beams.

  17. Generation of attosecond x-ray pulses with a multi-cycle two-color ESASE scheme

    SciTech Connect

    Ding, Y.; Huang, Z.; Ratner, D.; Bucksbaum, P.; Merdji, H.; /SLAC, PULSE

    2008-09-30

    Generation of attosecond x-ray pulses is attracting much attention within the x-ray free-electron laser (FEL) user community. Several schemes have been proposed based on manipulations of electron bunches with extremely short laser pulses. In this paper, we extend the attosecond two-color ESASE scheme proposed by Zholents et al. to the long optical cycle regime using a detuned second laser and a tapered undulator. Both lasers can be about ten-optical-cycles long, with the second laser frequency detuned from the first one to optimize the contrast between the central and side current spikes. A tapered undulator mitigates the degradation effect of the longitudinal space charge (LSC) force in the undulator and suppresses the FEL gain of all side current spikes. Simulations using the LCLS parameters show a single attosecond x-ray spike of {approx} 110 attosecond can be produced with a good contrast ratio.

  18. Table-top two-color soft X-ray laser by means of Ni-like plasmas

    NASA Astrophysics Data System (ADS)

    Masoudnia, Leili; Ruiz-Lopez, Mabel; Bleiner, Davide

    2016-04-01

    Laser-produced Ni-like plasmas are known as active media for extreme ultraviolet lasing, with the flexibility to two-color lasing. Two-color laser generation is very complex at accelerator facilities. In this work, plasma lasing at the 3d94d1(J = 0) → 3d94p1(J = 1) (collisional-pumping process) and the 3d94f1(J = 1) → 3d94d1(J = 1) (photo-pumping process) transitions is studied experimentally and computationally. Several key characteristics of collisional- and photo-pumping laser, such as divergence, pointing stability, and intensity have been investigated. The measurements showed different pulse characteristics for the two lasing processes affected by plasma inhomogeneity in temperature and density. Analytical expressions of these characteristics for both collisional- and photo-pumping are derived. It is found that the plasma that maximizes the photo-pumping lasing is 20% hotter and 70% denser than the plasma that optimizes the collisional-pumping lasing. The gain of collisional pumping is ≈4 times higher than the gain for the photo-pumping. The gain lifetime is a factor of ≈5.2 larger for the monopole-pumping. Similarly, the gain thickness is a factor of ≈1.8 larger. It is also found that the gain build-up time for collisional- and photo-pumping is 0.7 ps and 0.9 ps, respectively, whereas the build-up length-scale is 11.5 μm and 6.3 μm, respectively.

  19. Table-top two-color soft X-ray laser by means of Ni-like plasmas

    SciTech Connect

    Masoudnia, Leili; Ruiz-Lopez, Mabel; Bleiner, Davide

    2016-04-15

    Laser-produced Ni-like plasmas are known as active media for extreme ultraviolet lasing, with the flexibility to two-color lasing. Two-color laser generation is very complex at accelerator facilities. In this work, plasma lasing at the 3d{sup 9}4d{sup 1}(J = 0) → 3d{sup 9}4p{sup 1}(J = 1) (collisional-pumping process) and the 3d{sup 9}4f{sup 1}(J = 1) → 3d{sup 9}4d{sup 1}(J = 1) (photo-pumping process) transitions is studied experimentally and computationally. Several key characteristics of collisional- and photo-pumping laser, such as divergence, pointing stability, and intensity have been investigated. The measurements showed different pulse characteristics for the two lasing processes affected by plasma inhomogeneity in temperature and density. Analytical expressions of these characteristics for both collisional- and photo-pumping are derived. It is found that the plasma that maximizes the photo-pumping lasing is 20% hotter and 70% denser than the plasma that optimizes the collisional-pumping lasing. The gain of collisional pumping is ≈4 times higher than the gain for the photo-pumping. The gain lifetime is a factor of ≈5.2 larger for the monopole-pumping. Similarly, the gain thickness is a factor of ≈1.8 larger. It is also found that the gain build-up time for collisional- and photo-pumping is 0.7 ps and 0.9 ps, respectively, whereas the build-up length-scale is 11.5 μm and 6.3 μm, respectively.

  20. The 14/15 association as a paradigmatic example of tracing karyotype evolution in New World monkeys.

    PubMed

    Capozzi, Oronzo; Archidiacono, Nicoletta; Lorusso, Nicola; Stanyon, Roscoe; Rocchi, Mariano

    2016-09-01

    Fluorescence in situ hybridization (FISH), especially chromosome painting, has been extensively exploited in the phylogenetic reconstruction of primate evolution. Although chromosome painting is a key method to map translocations, it is not effective in detecting chromosome inversions, which may be up to four times more frequent than other chromosomal rearrangements. BAC-FISH instead can economically delineate marker order and reveal intrachromosomal rearrangements. However, up to now, BAC-FISH was rarely used to study the chromosomes of New World monkeys partly due to technical difficulties. In this paper, we used BAC-FISH to disentangle the complex evolutionary history of the ancestral 14/15 association in NWMs, beginning from the squirrel monkey (Saimiri boliviensis). To improve the hybridization efficiency of BAC-FISH in NWMs, we "translated" the human BACs into Callithrix jacchus (CJA) BACs, which yielded much higher hybridization efficiencies on other NWM species than human BACs. Our results disclosed 14 synteny blocks in squirrel monkeys, 7 more than with chromosome painting. We then applied a subset of CJA BACs on six other NWM species. The comparison of the hybridization pattern of these species contained phylogenetic information to discriminate evolutionary relationships. Notably Aotus was found to share an inversion with Callithrix, thus definitely assigning the genus Aotus to Cebidae. The present study can be seen as a paradigmatic approach to investigate the phylogenetics of NWMs by molecular cytogenetics.

  1. Momentum spectra of electrons rescattered from rare-gas targets following their extraction by one- and two-color femtosecond laser pulses

    SciTech Connect

    Ray, D.; Chen Zhangjin; De, S.; Cao, W.; Le, A. T.; Lin, C. D.; Cocke, C. L.; Litvinyuk, I. V.; Kling, M. F.

    2011-01-15

    We have used velocity-map imaging to measure the three-dimensional momenta of electrons rescattered from Xe and Ar following the liberation of the electrons from these atoms by 45 fs, 800 nm intense laser pulses. Strong structure in the rescattering region is observed in both angle and energy, and is interpreted in terms of quantitative rescattering (QRS) theory. Momentum images have also been taken with two-color (800 nm + 400 nm) pulses on Xe targets. A strong dependence of the spectra on the relative phase of the two colors is observed in the rescattering region. Interpretation of the phase dependence using both QRS theory and a full solution to the time-dependent Schroedinger equation shows that the rescattered electrons provide a much more robust method for determining the relative phase of the two colors than do the direct electrons.

  2. Two-color thermosensors based on [Y_{1-x}Dy_x(acetylacetonate)_3(1,10-phenanthroline)] molecular crystals

    NASA Astrophysics Data System (ADS)

    Anderson, Benjamin R.; Gunawidjaja, Ray; Eilers, Hergen

    2017-02-01

    We develop a two-color thermometry (TCT) phosphor based on [Y_{1-x}Dy_x(acetylacetonate)_3(1,10-phenanthroline)] ([Y_{1-x}Dy_x(acac)_3(phen)]) molecular crystals for use in heterogeneous materials. We characterize the optical properties of [Y_{1-x}Dy_x(acac)_3(phen)] crystals at different temperatures and Dy concentrations, and find that the emission is strongly quenched by increasing temperature and concentration. We also observe a broad background emission (due to the ligands) and find that [Y_{1-x}Dy_x(acac)_3(phen)] photodegrades under 355 nm illumination with the photodegradation resulting in decreased luminescence intensity. However, while decreasing the overall emission intensity, photodegradation is not found to influence the integrated intensity ratio of the ^4I_{15/2} → ^6H_{15/2} and ^4F_{9/2} → ^6H_{15/2} transitions. This ratio allows us to compute the temperature of the complex. Based on the temperature dependence of these ratios; we calculate that [Y_{1-x}Dy_x(acac)_3(phen)] has a maximum sensitivity of 1.5% K^{-1} and our TCT system has a minimum temperature resolution of 1.8 K. Finally, we demonstrate the use of [Y_{1-x}Dy_x(acac)_3(phen)] as a TCT phosphor by determining a dynamic temperature profile using the emission from [Y_{1-x}Dy_x(acac)_3(phen)].

  3. Two-color two-photon excited fluorescence of indole: determination of wavelength-dependent molecular parameters.

    PubMed

    Herbrich, Sebastian; Al-Hadhuri, Tawfik; Gericke, Karl-Heinz; Shternin, Peter S; Smolin, Andrey G; Vasyutinskii, Oleg S

    2015-01-14

    We present a detailed study of two-color two-photon excited fluorescence in indole dissolved in propylene glycol. Femtosecond excitation pulses at effective wavelengths from 268 to 293.33 nm were used to populate the two lowest indole excited states (1)La and (1)Lb and polarized fluorescence was then detected. All seven molecular parameters and the two-photon polarization ratio Ω containing information on two-photon absorption dynamics, molecular lifetime τf, and rotation correlation time τrot have been determined from experiment and analyzed as a function of the excitation wavelength. The analysis of the experimental data has shown that (1)Lb-(1)La inversion occurred under the conditions of our experiment. The two-photon absorption predominantly populated the (1)La state at all excitation wavelengths but in the 287-289 nm area which contained an absorption hump of the (1)Lb state 0-0 origin. The components of the two-photon excitation tensor S were analyzed giving important information on the principal tensor axes and absorption symmetry. The results obtained are in a good agreement with the results reported by other groups. The lifetime τf and the rotation correlation time τrot showed no explicit dependence on the effective excitation wavelength. Their calculated weighted average values were found to be τf = 3.83 ± 0.14 ns and τrot = 0.74 ± 0.06 ns.

  4. Route to optimal generation of soft X-ray high harmonics with synthesized two-color laser pulses

    PubMed Central

    Jin, Cheng; Wang, Guoli; Le, Anh-Thu; Lin, C. D.

    2014-01-01

    High harmonics extending to X-rays have been generated from gases by intense lasers. To establish these coherent broadband radiations as an all-purpose tabletop light source for general applications in science and technology, new methods are needed to overcome the present low conversion efficiencies. Here we show that the conversion efficiency may be drastically increased with an optimized two-color pulse. By employing an optimally synthesized 2-µm mid-infrared laser and a small amount of its third harmonic, we show that harmonic yields from sub- to few-keV energy can be increased typically by ten-fold over the optimized single-color one. By combining with favorable phase-matching and together with the emerging high-repetition MHz mid-infrared lasers, we anticipate efficiency of harmonic yields can be increased by four to five orders in the near future, thus paving the way for employing high harmonics as useful broadband tabletop light sources from the extreme ultraviolet to the X-rays, as well as providing new tools for interrogating ultrafast dynamics of matter at attosecond timescales. PMID:25400015

  5. Measurement of diffusion and thermal diffusion in ternary fluid mixtures using a two-color optical beam deflection technique

    NASA Astrophysics Data System (ADS)

    Königer, A.; Wunderlich, H.; Köhler, W.

    2010-05-01

    We have developed a highly sensitive two-color beam deflection setup to measure diffusion and thermal diffusion in ternary fluid mixtures following a suggestion of Haugen and Firoozabadi [J. Phys. Chem. B 110, 17678 (2006)]. Simultaneous detection of two laser beams with different wavelengths makes it possible to determine the time dependent concentration profiles of all three components. By comparing the measured beam deflection signals to a numerical solution of the coupled heat and mass transport equations, the diffusion matrix, the thermal diffusion, and the Soret coefficients are obtained by a numerical model combined with a nonlinear least-squares fitting routine. The results can be improved by additional thermal diffusion forced Rayleigh scattering experiments, which yield a contrast-weighted average thermal diffusion coefficient. The three Soret coefficients can be obtained independently from the stationary beam deflection amplitudes. Measurements have been performed on the symmetric (equal weight fractions) ternary mixtures dodecane/isobutylbenzene/1,2,3,4-tetrahydronaphthalene and 1-methylnaphthalene/octane/decane. There is only partial agreement between our results and literature data.

  6. Tunable Two-color Luminescence and Host–guest Energy Transfer of Fluorescent Chromophores Encapsulated in Metal-Organic Frameworks

    PubMed Central

    Yan, Dongpeng; Tang, Yanqun; Lin, Heyang; Wang, Dan

    2014-01-01

    Co-assembly of chromophore guests with host matrices can afford materials which have photofunctionalities different from those of individual components. Compared with clay and zeolite materials, the use of metal–organic frameworks (MOFs) as a host structure for fabricating luminescent host–guest materials is still at an early stage. Herein, we report the incorporation of a laser dye, 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran (DCM), into stilbene-based and naphthalene-based MOF systems. The resulting materials exhibit blue/red two-color emission, and the intensity ratio of blue to red fluorescence varies in different planes within the MOF crystal as detected by 3D confocal fluorescence microscopy. The observed changes in ratiometric fluorescence suggest the occurrence of energy transfer from MOF host to DCM molecules, which can be further confirmed by periodic density functional theoretical (DFT) calculations. Moreover, selective changes in luminescence behavior are observed on treating the guest@MOF samples with volatile organic compounds (methanol, acetone and toluene), indicating that these host–guest systems have potential applications as fluorescence sensors. It can be expected that by rational selection of MOF hosts and guest chromophores with suitable emissive colors and energy levels, a wide variety of multi-color luminescent and energy-transfer systems can readily be prepared in a similar manner. PMID:24614015

  7. Orientation dependence of the ionization of CO and NO in an intense femtosecond two-color laser field

    NASA Astrophysics Data System (ADS)

    Li, Hui; Ray, Dipanwita; de, Sankar; Cao, Wei; Laurent, Guillaume; Wang, Zhenhua; Thu Le, Anh; Cocke, C. Lewis; Znakovskaya, Irina; Kling, Matthias

    2012-06-01

    Two-color (800 nm and 400 nm) ultrashort (30±10 fs) laser pulses were used to ionize and dissociate CO and NO. The emission of C^+q, N^+q and O^+ fragments were measured with a velocity-map-imaging (VMI) system. The data show that the ionization rate is dependent on the orientation of the molecules with respect to the laser polarization. Both molecules ionize more easily when the electric field points from C to O in CO and from N to O in NO. The asymmetry of emission is much higher for CO than for NO. The sign of the asymmetry is not strongly dependent on kinetic energy release (KER). The favored ionization orientation is in agreement with the expectation of the molecular orbital Ammosov-Delone-Krainov (MO-ADK) [1] theory and with a Stark-corrected version of a strong-field-approximation (SFA) calculation [2]. [4pt] [1] X.M. Tong, et al., Phys. Rev. A 66, 033402 (2002).[0pt] [2] H. Li, et al., Phys. Rev. A 84, 043429 (2011).

  8. SPECTROSCOPIC CONFIRMATION OF UV-BRIGHT WHITE DWARFS FROM THE SANDAGE TWO-COLOR SURVEY OF THE GALACTIC PLANE

    SciTech Connect

    Lepine, Sebastien; Bergeron, P.; Lanning, Howard H.

    2011-03-15

    We present spectroscopic observations confirming the identification of hot white dwarfs among UV-bright sources from the Sandage Two-color Survey of the Galactic Plane and listed in the Lanning (Lan) catalog of such sources. A subsample of 213 UV-bright Lan sources have been identified as candidate white dwarfs based on the detection of a significant proper motion. Spectroscopic observations of 46 candidates with the KPNO 2.1 m telescope confirm 30 sources to be hydrogen white dwarfs with subtypes in the DA1-DA6 range, and with one of the stars (Lan 161) having an unresolved M dwarf as a companion. Five more sources are confirmed to be helium white dwarfs, with subtypes from DB3 to DB6. One source (Lan 364) is identified as a DZ 3 white dwarf, with strong lines of calcium. Three more stars are found to have featureless spectra (to within detection limits) and are thus classified as DC white dwarfs. In addition, three sources are found to be hot subdwarfs: Lan 20 and Lan 480 are classified as sdOB, and Lan 432 is classified sdB. The remaining four objects are found to be field F star interlopers. Physical parameters of the DA and DB white dwarfs are derived from model fits.

  9. Two-color vibrational, femtosecond, fully resonant electronically enhanced CARS (FREE-CARS) of gas-phase nitric oxide.

    PubMed

    Stauffer, Hans U; Roy, Sukesh; Schmidt, Jacob B; Wrzesinski, Paul J; Gord, James R

    2016-09-28

    A resonantly enhanced, two-color, femtosecond time-resolved coherent anti-Stokes Raman scattering (CARS) approach is demonstrated and used to explore the nature of the frequency- and time-dependent signals produced by gas-phase nitric oxide (NO). Through careful selection of the input pulse wavelengths, this fully resonant electronically enhanced CARS (FREE-CARS) scheme allows rovibronic-state-resolved observation of time-dependent rovibrational wavepackets propagating on the vibrationally excited ground-state potential energy surface of this diatomic species. Despite the use of broadband, ultrafast time-resolved input pulses, high spectral resolution of gas-phase rovibronic transitions is observed in the FREE-CARS signal, dictated by the electronic dephasing timescales of these states. Analysis and computational simulation of the time-dependent spectra observed as a function of pump-Stokes and Stokes-probe delays provide insight into the rotationally resolved wavepacket motion observed on the excited-state and vibrationally excited ground-state potential energy surfaces of NO, respectively.

  10. Optimized two-color super resolution imaging of Drp1 during mitochondrial fission with a slow-switching Dronpa variant.

    PubMed

    Rosenbloom, Alyssa B; Lee, Sang-Hyuk; To, Milton; Lee, Antony; Shin, Jae Yen; Bustamante, Carlos

    2014-09-09

    We studied the single-molecule photo-switching properties of Dronpa, a green photo-switchable fluorescent protein and a popular marker for photoactivated localization microscopy. We found the excitation light photoactivates as well as deactivates Dronpa single molecules, hindering temporal separation and limiting super resolution. To resolve this limitation, we have developed a slow-switching Dronpa variant, rsKame, featuring a V157L amino acid substitution proximal to the chromophore. The increased steric hindrance generated by the substitution reduced the excitation light-induced photoactivation from the dark to fluorescent state. To demonstrate applicability, we paired rsKame with PAmCherry1 in a two-color photoactivated localization microscopy imaging method to observe the inner and outer mitochondrial membrane structures and selectively labeled dynamin related protein 1 (Drp1), responsible for membrane scission during mitochondrial fission. We determined the diameter and length of Drp1 helical rings encircling mitochondria during fission and showed that, whereas their lengths along mitochondria were not significantly changed, their diameters decreased significantly. These results suggest support for the twistase model of Drp1 constriction, with potential loss of subunits at the helical ends.

  11. Parametric study of broadband terahertz radiation generation based on interaction of two-color ultra-short laser pulses

    SciTech Connect

    Moradi, S.; Ganjovi, A.; Shojaei, F.; Saeed, M.

    2015-04-15

    In this work, using a two-dimensional kinetic model based on particle in cell-Monte Carlo collision simulation method, the influence of different parameters on the broadband intense Terahertz (THz) radiation generation via application of two-color laser fields, i.e., the fundamental and second harmonic modes, is studied. These two modes are focused into the molecular oxygen (O{sub 2}) with uniform density background gaseous media and the plasma channels are created. Thus, a broadband THz pulse that is around the plasma frequency is emitted from the formed plasma channel and co-propagates with the laser pulse. For different laser pulse shapes, the THz electric field and its spectrum are both calculated. The effects of laser pulse and medium parameters, i.e., positive and negative chirp pulse, number of laser cycles in the pulse, laser pulse shape, background gas pressure, and exerted DC electric field on THz spectrum are verified. Application of a negatively chirped femtosecond (40 fs) laser pulse results in four times enhancement of the THz pulse energy (2 times in THz electric field). The emission of THz radiation is mostly observed in the forward direction.

  12. Two-color widefield fluorescence microendoscopy enables multiplexed molecular imaging in the alveolar space of human lung tissue

    NASA Astrophysics Data System (ADS)

    Krstajić, Nikola; Akram, Ahsan R.; Choudhary, Tushar R.; McDonald, Neil; Tanner, Michael G.; Pedretti, Ettore; Dalgarno, Paul A.; Scholefield, Emma; Girkin, John M.; Moore, Anne; Bradley, Mark; Dhaliwal, Kevin

    2016-04-01

    We demonstrate a fast two-color widefield fluorescence microendoscopy system capable of simultaneously detecting several disease targets in intact human ex vivo lung tissue. We characterize the system for light throughput from the excitation light emitting diodes, fluorescence collection efficiency, and chromatic focal shifts. We demonstrate the effectiveness of the instrument by imaging bacteria (Pseudomonas aeruginosa) in ex vivo human lung tissue. We describe a mechanism of bacterial detection through the fiber bundle that uses blinking effects of bacteria as they move in front of the fiber core providing detection of objects smaller than the fiber core and cladding (˜3 μm). This effectively increases the measured spatial resolution of 4 μm. We show simultaneous imaging of neutrophils, monocytes, and fungus (Aspergillus fumigatus) in ex vivo human lung tissue. The instrument has 10 nM and 50 nM sensitivity for fluorescein and Cy5 solutions, respectively. Lung tissue autofluorescence remains visible at up to 200 fps camera acquisition rate. The optical system lends itself to clinical translation due to high-fluorescence sensitivity, simplicity, and the ability to multiplex several pathological molecular imaging targets simultaneously.

  13. Two-color widefield fluorescence microendoscopy enables multiplexed molecular imaging in the alveolar space of human lung tissue.

    PubMed

    Krstajic, Nikola; Akram, Ahsan R; Choudhary, Tushar R; McDonald, Neil; Tanner, Michael G; Pedretti, Ettore; Dalgarno, Paul A; Scholefield, Emma; Girkin, John M; Moore, Anne; Bradley, Mark; Dhaliwal, Kevin

    2016-04-30

    We demonstrate a fast two-color widefield fluorescence microendoscopy system capable of simultaneously detecting several disease targets in intact human ex vivo lung tissue. We characterize the system for light throughput from the excitation light emitting diodes, fluorescence collection efficiency, and chromatic focal shifts. We demonstrate the effectiveness of the instrument by imaging bacteria (Pseudomonas aeruginosa) in ex vivo human lung tissue. We describe a mechanism of bacterial detection through the fiber bundle that uses blinking effects of bacteria as they move in front of the fiber core providing detection of objects smaller than the fiber core and cladding (∼3  μm ∼3  μm ). This effectively increases the measured spatial resolution of 4  μm 4  μm . We show simultaneous imaging of neutrophils, monocytes, and fungus (Aspergillus fumigatus) in ex vivo human lung tissue. The instrument has 10 nM and 50 nM sensitivity for fluorescein and Cy5 solutions, respectively. Lung tissue autofluorescence remains visible at up to 200 fps camera acquisition rate. The optical system lends itself to clinical translation due to high-fluorescence sensitivity, simplicity, and the ability to multiplex several pathological molecular imaging targets simultaneously.

  14. Electronic and molecular properties of an adsorbed protein monolayer probed by two-color sum-frequency generation spectroscopy.

    PubMed

    Dreesen, L; Humbert, C; Sartenaer, Y; Caudano, Y; Volcke, C; Mani, A A; Peremans, A; Thiry, P A; Hanique, S; Frère, J-M

    2004-08-17

    Two-color sum-frequency generation spectroscopy (2C-SFG) is used to probe the molecular and electronic properties of an adsorbed layer of the green fluorescent protein mutant 2 (GFPmut2) on a platinum (111) substrate. First, the spectroscopic measurements, performed under different polarization combinations, and atomic force microscopy (AFM) show that the GFPmut2 proteins form a fairly ordered monolayer on the platinum surface. Next, the nonlinear spectroscopic data provide evidence of particular coupling phenomena between the GFPmut2 vibrational and electronic properties. This is revealed by the occurrence of two doubly resonant sum-frequency generation processes for molecules having both their Raman and infrared transition moments in a direction perpendicular to the sample plane. Finally, our 2C-SFG analysis reveals two electronic transitions corresponding to the absorption and fluorescence energy levels which are related to two different GFPmut2 conformations: the B (anionic) and I forms, respectively. Their observation and wavelength positions attest the keeping of the GFPmut2 electronic properties upon adsorption on the metallic surface.

  15. Selective bond breaking of CO2 in phase-locked two-color intense laser fields: laser field intensity dependence.

    PubMed

    Endo, Tomoyuki; Fujise, Hikaru; Kawachi, Yuuna; Ishihara, Ayaka; Matsuda, Akitaka; Fushitani, Mizuho; Kono, Hirohiko; Hishikawa, Akiyoshi

    2017-02-01

    Selective bond breaking of CO2 in phase-locked ω-2ω two-color intense laser fields (λ = 800 nm and 400 nm, total field intensity I ∼ 10(14) W cm(-2)) has been investigated by coincidence momentum imaging. The CO(+) and O(+) fragment ions produced by two-body Coulomb explosion, CO2(2+) → CO(+) + O(+), exhibit asymmetric distributions along the laser polarization direction, showing that one of the two equivalent C-O bonds is selectively broken by the laser fields. At a field intensity higher than 2 × 10(14) W cm(-2), the largest fragment asymmetry is observed when the relative phase ϕ between the ω and 2ω laser fields is ∼0 and π. On the other hand, an increase of the asymmetry and a shift of the phase providing the largest asymmetry are observed at lower field intensities. The selective bond breaking and its dependence on the laser field intensity are discussed in terms of a mechanism involving deformation of the potential energy surfaces and electron recollision in intense laser fields.

  16. Two-color cytofluorometry and cellular properties of the urokinase receptor associated with a human metastatic carcinomatous cell line

    SciTech Connect

    Takahashi, K.; Gojobori, T.; Tanifuji, M. )

    1991-02-01

    Purified human urokinase was labeled with either fluorescein isothiocyanate or iodine-125 and used as a probe for binding to the human metastatic carcinomatous cell line, Detroit 562. Cytofluorometry showed that the ligand bound preferentially to cells that had been exposed to acidic pH. The binding was competitive and decreased after mild tryptic digestion. The bound ligand could be removed by restoration of the cells to a low pH. Therefore, the cells had specific binding sites. The bound urokinase was involved in the breakdown of fibrin. Two-color cytofluorometric maps were constructed by counterstaining with propidium iodide. Results suggested that there were different cell populations that had different numbers of receptors and amounts of DNA. We cloned cells and found that single clones had homogeneous levels of receptors with different dissociation constants (from 10(-13) to 10(-11) mol/mg protein) for different clones. Cells of one clone, C5, which had high levels of receptor production, moved characteristically on a glass substratum coated with gold particles and reacted with wheat germ agglutinin, but not with concanavalin A. The receptors were found together with adhesion proteins at the sites where the cells adhered to the substrate. These results and the data obtained by zymography of the cellular proteins suggested that the urokinase-type plasminogen activators were bound to the receptors. The membrane-associated activator may stimulate local proteolysis, facilitating the migration of the tumor cell across the substrate.

  17. Route to optimal generation of soft X-ray high harmonics with synthesized two-color laser pulses.

    PubMed

    Jin, Cheng; Wang, Guoli; Le, Anh-Thu; Lin, C D

    2014-11-17

    High harmonics extending to X-rays have been generated from gases by intense lasers. To establish these coherent broadband radiations as an all-purpose tabletop light source for general applications in science and technology, new methods are needed to overcome the present low conversion efficiencies. Here we show that the conversion efficiency may be drastically increased with an optimized two-color pulse. By employing an optimally synthesized 2-µm mid-infrared laser and a small amount of its third harmonic, we show that harmonic yields from sub- to few-keV energy can be increased typically by ten-fold over the optimized single-color one. By combining with favorable phase-matching and together with the emerging high-repetition MHz mid-infrared lasers, we anticipate efficiency of harmonic yields can be increased by four to five orders in the near future, thus paving the way for employing high harmonics as useful broadband tabletop light sources from the extreme ultraviolet to the X-rays, as well as providing new tools for interrogating ultrafast dynamics of matter at attosecond timescales.

  18. Single attosecond pulse generation in an orthogonally polarized two-color laser field combined with a static electric field

    SciTech Connect

    Xia Changlong; Zhang Gangtai; Wu Jie; Liu Xueshen

    2010-04-15

    We investigate theoretic high-order harmonic generation and single attosecond pulse generation in an orthogonally polarized two-color laser field, which is synthesized by a mid-infrared (IR) pulse (12.5 fs, 2000 nm) in the y component and a much weaker (12 fs, 800 nm) pulse in the x component. We find that the width of the harmonic plateau can be extended when a static electric field is added in the y component. We also investigate emission time of harmonics in terms of a time-frequency analysis to illustrate the physical mechanism of high-order harmonic generation. We calculate the ionization rate using the Ammosov-Delone-Krainov model and interpret the variation of harmonic intensity for different static electric field strengths. When the ratio of strengths of the static and the y-component laser fields is 0.1, a continuous harmonic spectrum is formed from 220 to 420 eV. By superposing a properly selected range of the harmonic spectrum from 300 to 350 eV, an isolated attosecond pulse with a duration of about 75 as is obtained, which is near linearly polarized.

  19. Generation of attosecond x-ray pulses with a multi-cycle two-color ESASE scheme

    SciTech Connect

    Ding, Y.; Huang, Z.; Ratner, D.; Bucksbaum, P.; Merdji, H.; /Saclay /SLAC

    2009-03-04

    Generation of attosecond x-ray pulses is attracting much attention within the x-ray free-electron laser (FEL) user community. Several schemes using extremely short laser pulses to manipulate the electron bunches have been proposed. In this paper, we extend the attosecond two-color ESASE scheme proposed by Zholents et al. to the long optical cycle regime using a second detuned laser and a tapered undulator. Both lasers can be about ten-optical-cycles long, with the second laser frequency detuned from the first to optimize the contrast between the central and side current spikes. A tapered undulator mitigates the degradation effect of the longitudinal space charge (LSC) force in the undulator and suppresses the FEL gain of all side current peaks. Simulations using the LCLS parameters show a single attosecond x-ray spike of {approx} 110 attoseconds can be produced. The second laser can also be detuned to coherently control the number of the side x-ray spikes and the length of the radiation pulse.

  20. Two-color vibrational, femtosecond, fully resonant electronically enhanced CARS (FREE-CARS) of gas-phase nitric oxide

    NASA Astrophysics Data System (ADS)

    Stauffer, Hans U.; Roy, Sukesh; Schmidt, Jacob B.; Wrzesinski, Paul J.; Gord, James R.

    2016-09-01

    A resonantly enhanced, two-color, femtosecond time-resolved coherent anti-Stokes Raman scattering (CARS) approach is demonstrated and used to explore the nature of the frequency- and time-dependent signals produced by gas-phase nitric oxide (NO). Through careful selection of the input pulse wavelengths, this fully resonant electronically enhanced CARS (FREE-CARS) scheme allows rovibronic-state-resolved observation of time-dependent rovibrational wavepackets propagating on the vibrationally excited ground-state potential energy surface of this diatomic species. Despite the use of broadband, ultrafast time-resolved input pulses, high spectral resolution of gas-phase rovibronic transitions is observed in the FREE-CARS signal, dictated by the electronic dephasing timescales of these states. Analysis and computational simulation of the time-dependent spectra observed as a function of pump-Stokes and Stokes-probe delays provide insight into the rotationally resolved wavepacket motion observed on the excited-state and vibrationally excited ground-state potential energy surfaces of NO, respectively.

  1. Optimized two-color super resolution imaging of Drp1 during mitochondrial fission with a slow-switching Dronpa variant

    PubMed Central

    Rosenbloom, Alyssa B.; Lee, Sang-Hyuk; To, Milton; Lee, Antony; Shin, Jae Yen; Bustamante, Carlos

    2014-01-01

    We studied the single-molecule photo-switching properties of Dronpa, a green photo-switchable fluorescent protein and a popular marker for photoactivated localization microscopy. We found the excitation light photoactivates as well as deactivates Dronpa single molecules, hindering temporal separation and limiting super resolution. To resolve this limitation, we have developed a slow-switching Dronpa variant, rsKame, featuring a V157L amino acid substitution proximal to the chromophore. The increased steric hindrance generated by the substitution reduced the excitation light-induced photoactivation from the dark to fluorescent state. To demonstrate applicability, we paired rsKame with PAmCherry1 in a two-color photoactivated localization microscopy imaging method to observe the inner and outer mitochondrial membrane structures and selectively labeled dynamin related protein 1 (Drp1), responsible for membrane scission during mitochondrial fission. We determined the diameter and length of Drp1 helical rings encircling mitochondria during fission and showed that, whereas their lengths along mitochondria were not significantly changed, their diameters decreased significantly. These results suggest support for the twistase model of Drp1 constriction, with potential loss of subunits at the helical ends. PMID:25149858

  2. Two-color two-photon excited fluorescence of indole: Determination of wavelength-dependent molecular parameters

    NASA Astrophysics Data System (ADS)

    Herbrich, Sebastian; Al-Hadhuri, Tawfik; Gericke, Karl-Heinz; Shternin, Peter S.; Smolin, Andrey G.; Vasyutinskii, Oleg S.

    2015-01-01

    We present a detailed study of two-color two-photon excited fluorescence in indole dissolved in propylene glycol. Femtosecond excitation pulses at effective wavelengths from 268 to 293.33 nm were used to populate the two lowest indole excited states 1La and 1Lb and polarized fluorescence was then detected. All seven molecular parameters and the two-photon polarization ratio Ω containing information on two-photon absorption dynamics, molecular lifetime τf, and rotation correlation time τrot have been determined from experiment and analyzed as a function of the excitation wavelength. The analysis of the experimental data has shown that 1Lb-1La inversion occurred under the conditions of our experiment. The two-photon absorption predominantly populated the 1La state at all excitation wavelengths but in the 287-289 nm area which contained an absorption hump of the 1Lb state 0-0 origin. The components of the two-photon excitation tensor S were analyzed giving important information on the principal tensor axes and absorption symmetry. The results obtained are in a good agreement with the results reported by other groups. The lifetime τf and the rotation correlation time τrot showed no explicit dependence on the effective excitation wavelength. Their calculated weighted average values were found to be τf = 3.83 ± 0.14 ns and τrot = 0.74 ± 0.06 ns.

  3. Origin of the Apollo 14, 15, and 17 yellow ultramafic glasses by mixing of deep cumulate remelts

    NASA Astrophysics Data System (ADS)

    Brown, S. M.; Grove, T. L.

    2015-12-01

    We examine the fO2-dependent melting conditions of the Apollo 14 yellow intermediate-Ti ultramafic glasses and the melting processes that formed the full suite of lunar yellow ultramafic glasses. Multiple saturation experiments indicate that the Apollo 14 yellow glass would have been in equilibrium with residual olivine and low-Ca pyroxene near 1530 °C and 2.4 GPa at ΔIW = +2. At ΔIW = -2, the multiple saturation point moves to greater depth and higher temperature to 1580 °C and 3.0 GPa. Combining the results of this study with that of Krawczynski and Grove (2012) on more Ti-rich Apollo orange and red glass indicates that the fO2-induced change in multiple saturation pressure correlates with the Fe-Ti# (molar (FeO + TiO2*)/(MgO + FeO + TiO2*), where TiO2* = all Ti calculated as Ti4+) of the liquid. Further, a decrease in the olivine Fe-Mg exchange coefficient at lower fO2 suggests that Fe2+ is complexing more efficiently with Ti3+ at the expense of Mg in the melt than it did with Ti4+ at higher fO2. Processes involving assimilation and/or fractionation and/or melt-wall rock reaction all fail to produce the within-suite compositional variability observed in the Apollo 14, 15, and 17 yellow glasses. Mixing of remelted source cumulates, combined with small amounts of olivine fractionation, are the only mechanisms that can reproduce all three trends. We present a quantitative model of the mixing process with simultaneous olivine fractionation. Remarkably, the trends can be explained by mixing melts of an ultramafic source (olivine + pigeonite or orthopyroxene), a clinopyroxene + ilmenite bearing cumulate, and KREEP. Lunar mantle overturn is the most likely process that can reconcile the observed major and trace element compositional characteristics and the experimental results. These two constraints are consistent with different models of lunar magma ocean crystallization (Snyder et al., 1992; Eklins-Tanton et al., 2011). A complex, hot thermal history is necessary

  4. Phototransistor (PT) in the 2 Micron Region

    NASA Technical Reports Server (NTRS)

    Prather, Dennis; Sulima, Oleg V.

    2006-01-01

    Within the framework of the project the University of Delaware has developed InGaAsSb-based heterojunction phototransistors (HPT) structure with a large (1000 micron diameter) photosensitive/photoactive area. Two different compositions of quaternary alloys were used to provide the cutoff wavelength (50% of maximum quantum efficiency) of 2.4 micron (Type 1) and 2.15 micron (Type 2). The Type 1 HPT was composed of Al0.25Ga0.75As0.02Sb0.98 and In0.18Ga0.82As0.17Sb0.83 layers with room-temperature bandgaps of Eg approximates 1.0 eV and Eg approximates 0.54 eV, respectively. The layers are lattice-matched to a GaSb substrate. The growth started with a 0.15micron-thick n+-GaSb buffer layer and was completed with a 0.1 m-thick n+- GaSb contact layer doped with Te. The HPT structure includes a 0.5 m-thick n-type AlGaAsSb emitter, 0.8 micron-thick p-type composite base consisting of AlGaAsSb (0.3 m) and InGaAsSb (0.5 m) layers, and a 1.5micron - thick n type InGaAsSb collector. The Type 2 HPT differed by a higher bandgap In0.16Ga0.84As 0.14Sb0.86 layers with a room-temperature bandgap of Eg approximates 0.555 eV.

  5. Exploratory 5-micron spectrum of Uranus

    SciTech Connect

    Orton, G.S.; Kaminski, C.D.

    1989-01-01

    The intensity peak at 4.8 microns characterizing the spectrum observed for the disk of Uranus near 5 microns, in June 1987, exhibits steep declines at shorter and longer wavelength. An exploratory discussion is presented of various models in view of these data; it is noted that some component of the radiation must originate near the 140 K atmospheric irrespective of the radiation's origin in sunlight or thermal emission; physical considerations dictate that it be at least partly thermal in origin. One model consistent with the data requires the presence of a cloud top at the 8-bar level. 30 references.

  6. Interpreting the 10 micron Astronomical Silicate Feature

    NASA Astrophysics Data System (ADS)

    Bowey, Janet E.

    1998-11-01

    10micron spectra of silicate dust in the diffuse medium towards Cyg OB2 no. 12 and towards field and embedded objects in the Taurus Molecular Cloud (TMC) were obtained with CGS3 at the United Kingdom Infrared Telescope (UKIRT). Cold molecular-cloud silicates are sampled in quiescent lines of sight towards the field stars Taurus-Elias 16 and Elias 13, whilst observations of the embedded young stellar objects HL Tau, Taurus-Elias 7 (Haro6-10) and Elias 18 also include emission from heated dust. To obtain the foreground silicate absorption profiles, featureless continua are estimated using smoothed astronomical and laboratory silicate emissivities. TMC field stars and Cyg OB2 no. 12 are modelled as photospheres reddened by foreground continuum and silicate extinction. Dust emission in the non-photospheric continua of HL Tau and Elias 7 (Haro6-10) is distinguished from foreground silicate absorption using a 10micron disk model, based on the IR-submm model of T Tauri stars by Adams, Lada & Shu (1988), with terms added to represent the foreground continuum and silicate extinction. The absorption profiles of HL Tau and Elias 7 are similar to that of the field star Elias 16. Fitted temperature indices of 0.43 (HL Tau) and 0.33 (Elias 7) agree with Boss' (1996) theoretical models of the 200-300K region, but are lower than those of IR-submm disks (0.5-0.61; Mannings & Emerson 1994); the modelled 10micron emission of HL Tau is optically thin, that of Elias 7 is optically thick. A preliminary arcsecond-resolution determination of the 10micron emissivity near θ1 Ori D in the Trapezium region of Orion and a range of emission temperatures (225-310K) are derived from observations by T. L. Hayward; this Ney-Allen emissivity is 0.6micron narrower than the Trapezium emissivity obtained by Forrest et al. (1975) with a large aperture. Published interstellar grain models, elemental abundances and laboratory studies of Solar System silicates (IDPs, GEMS and meteorites), the 10micron

  7. Perfecting the Formula: Effective Strategies = Educational Success. A Report from the 2009 Governors Education Symposium (Cary, North Carolina, June 14-15, 2009)

    ERIC Educational Resources Information Center

    NGA Center for Best Practices, 2009

    2009-01-01

    The 2009 "Governors Education Symposium" was co-hosted by the James B. Hunt, Jr. Institute for Educational Leadership and Policy and the National Governors Association Center for Best Practices on June 14-15, 2009, in Cary, North Carolina. Vermont Governor Jim Douglas and former North Carolina Governor Jim Hunt served as co-chairs. This year's…

  8. University-School Partnerships: Polymer Chemistry Days Run at a University for 14-15 Year Olds and Their Impact on Attitudes to Science

    ERIC Educational Resources Information Center

    Shaw, A. J.; Harrison, T. G.; Medley, M. I.; Sellou, L.; Shallcross, K. L.; Croker, S. J.; Williams, S. J.; Shallcross, D. E.

    2010-01-01

    Polymer Chemistry days run by Bristol ChemLabS at the School of Chemistry, University of Bristol for year 10 (14-15 year olds) school students are described. Pre and post questionnaires were analysed to determine the impact on attitudes to science. There was no change in attitudes to the importance of science or practical work in science, but…

  9. Alchornea cordifolia seed oil: A rich source of a new C20 epoxide, (+)cis-14,15-epoxy-cis-11-eicosenoic acid.

    PubMed

    Kleiman, R; Plattner, R D; Spencer, G F

    1977-07-01

    A C20 homolog of vernolic acid has been found at the 50% level inAlchornea cordifolia, Euphorbiaceae, seed oil. This new acid, (+)cis-14,15-epoxy-cis-11-eicosenoic (alchornoic) acid, was isolated by high-pressure liquid chromotography and characterized by mass spectrometry, nuclear magnetic resonance and infrared spectroscopy, optical rotary dispersion, and ozonolysis-gas-chromotography.

  10. Proceedings, sixth international symposium : moisture and creep effects on paper, board and containers: Madison, Wisconsin, USA, 14-15 July 2009

    Treesearch

    John M. Considine; Sally A. Ralph

    2011-01-01

    The USDA Forest Products Laboratory sponsored the 6th International Symposium: Moisture and Creep Effects on Paper, Board and Containers at the Monona Terrace Convention Center, Madison, WI, USA on 14-15 July 2009. Attendees heard 20 technical presentations; presenters were from seven different countries and three continents. Session topics included Corrugated...

  11. Proceedings of the National Conference on Population Library and Information Services (3rd Chapel Hill, N. C., May 14-15, 1970)

    ERIC Educational Resources Information Center

    Kellermann, Priscilla

    Contains the proceedings of the Third National Conference on Population Library and Information Services held in Chapel Hill, North Carolina, May 14-15, 1970. Under the joint sponsorship of the Carolina Population Center and the Population Council of New York, this was the third in a series of annual conference/workshops devoted to library and…

  12. 40 CFR 721.10284 - Poly[oxy(methyl-1,2-ethanediyl)], .alpha.-sulfo-.omega.-hydroxy-, C14-15-branched and linear...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... CHEMICAL SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.10284 Poly , .alpha.-sulfo-.omega.-hydroxy-, C14-15-branched and linear alkyl ethers, sodium salts. (a) Chemical substance and significant new uses subject to reporting. (1) The chemical substance identified as poly ,...

  13. Fabrication of two-color annular hybrid wave plate for three-dimensional super-resolution microscopy

    NASA Astrophysics Data System (ADS)

    Kumagai, Hiroshi; Iketaki, Yoshinori; Jahn, Kornel; Bokor, Nador

    2016-03-01

    In super-resolution microscopy, we use fluorescence depletion, where an erase beam quenches a molecule in the S1 state generated by a pump beam, and then prevents fluorescence from the S1 state. When a tight doughnut shaped erase beam with is focused on the dyed sample together with a Gaussian pump beam, the remaining fluorescence spot in the focal plane becomes smaller than the diffraction-limited size. Applying destructive interference to the erase beam, erase beam has a minute three-dimensional dark spot surrounded by the light near the focal region. Since this spot introduces fluorescence depletion along the optical axis as in the focal plane, we can achieve three-dimensional super-resolution microscopy. However, to overcome the diffraction limit, an extremely precise optical alignment is required for projecting the focused pump beam into the dark spot of the erase beam. To resolve this technical issue, we fabricated a two-color annular hybrid wave plate (TAHWP) by combining two multi-order wave quartz plates. Although the pump and erase beams co-axially pass through the plate; the pump beam retains its original Gaussian shape, while the erase beam undergoes destructive interference. Inserting the TAHWP into a commercial scanning laser microscope, a three-dimensional spherical fluorescence spot with a volume of (~100 nm)3 can be created. Beside eliminating alignment problems and yielding a compact setup, the TAHWP makes our proposed method very suitable for commercial microscope systems. In this study, we report about detailed fabrication procedure and three-dimensional image properties given by the TAHWP.

  14. Two-color two-photon excited fluorescence of indole: Determination of wavelength-dependent molecular parameters

    SciTech Connect

    Herbrich, Sebastian; Al-Hadhuri, Tawfik; Gericke, Karl-Heinz; Shternin, Peter S. Vasyutinskii, Oleg S.; Smolin, Andrey G.

    2015-01-14

    We present a detailed study of two-color two-photon excited fluorescence in indole dissolved in propylene glycol. Femtosecond excitation pulses at effective wavelengths from 268 to 293.33 nm were used to populate the two lowest indole excited states {sup 1}L{sub a} and {sup 1}L{sub b} and polarized fluorescence was then detected. All seven molecular parameters and the two-photon polarization ratio Ω containing information on two-photon absorption dynamics, molecular lifetime τ{sub f}, and rotation correlation time τ{sub rot} have been determined from experiment and analyzed as a function of the excitation wavelength. The analysis of the experimental data has shown that {sup 1}L{sub b}–{sup 1}L{sub a} inversion occurred under the conditions of our experiment. The two-photon absorption predominantly populated the {sup 1}L{sub a} state at all excitation wavelengths but in the 287–289 nm area which contained an absorption hump of the {sup 1}L{sub b} state 0-0 origin. The components of the two-photon excitation tensor S were analyzed giving important information on the principal tensor axes and absorption symmetry. The results obtained are in a good agreement with the results reported by other groups. The lifetime τ{sub f} and the rotation correlation time τ{sub rot} showed no explicit dependence on the effective excitation wavelength. Their calculated weighted average values were found to be τ{sub f} = 3.83 ± 0.14 ns and τ{sub rot} = 0.74 ± 0.06 ns.

  15. Coherent control of ionization in two-color laser fields: a comparison between /ω-/2ω and /ω-/3ω combinations

    NASA Astrophysics Data System (ADS)

    Gong, Shang-qing; Wu, Hai-tao; Wang, Zhong-yang; Xu, Zhi-zhan

    2000-07-01

    Using the numerical solutions of Schrödinger equation with a model potential, we investigate the coherent control of ionization in two-color fields. Comparing the ω-2 ω combination with the ω-3 ω scenario, we find that the ionization rates of system are quite different due to the different superposition effects of the two kinds of combinations.

  16. Sub-micron particle sampler apparatus and method for sampling sub-micron particles

    DOEpatents

    Gay, D.D.; McMillan, W.G.

    1984-04-12

    Apparatus and method steps for collecting sub-micron sized particles include a collection chamber and cryogenic cooling. The cooling is accomplished by coil tubing carrying nitrogen in liquid form, with the liquid nitrogen changing to the gas phase before exiting from the collection chamber in the tubing. Standard filters are used to filter out particles of diameter greater than or equal to 0.3 microns; however, the present invention is used to trap particles of less than 0.3 micron in diameter. A blower draws air to said collection chamber through a filter which filters particles with diameters greater than or equal to 0.3 micron. The air is then cryogenically cooled so that moisture and sub-micron sized particles in the air condense into ice on the coil. The coil is then heated so that the ice melts, and the liquid is then drawn off and passed through a Buchner funnel where the liquid is passed through a Nuclepore membrane. A vacuum draws the liquid through the Nuclepore membrane, with the Nuclepore membrane trapping sub-micron sized particles therein. The Nuclepore membrane is then covered on its top and bottom surfaces with sheets of Mylar and the assembly is then crushed into a pellet. This effectively traps the sub-micron sized particles for later analysis. 6 figures.

  17. Application of coherent 10 micron imaging lidar

    SciTech Connect

    Simpson, M.L.; Hutchinson, D.P.; Richards, R.K.; Bennett, C.A.

    1997-04-01

    With the continuing progress in mid-IR array detector technology and high bandwidth fan-outs, i.f. electronics, high speed digitizers, and processing capability, true coherent imaging lidar is becoming a reality. In this paper experimental results are described using a 10 micron coherent imaging lidar.

  18. The 11 Micron Emissions of Carbon Stars

    NASA Technical Reports Server (NTRS)

    Goebel, J. H.; Cheeseman, P.; Gerbault, F.

    1995-01-01

    A new classification scheme of the IRAS LRS carbon stars is presented. It comprises the separation of 718 probable carbon stars into 12 distinct self-similar spectral groupings. Continuum temperatures are assigned and range from 470 to 5000 K. Three distinct dust species are identifiable: SiC, alpha:C-H, and MgS. In addition to the narrow 11 + micron emission feature that is commonly attributed to SiC, a broad 11 + micron emission feature, that is correlated with the 8.5 and 7.7 micron features, is found and attributed to alpha:C-H. SiC and alpha:C-H band strengths are found to correlate with the temperature progression among the Classes. We find a spectral sequence of Classes that reflects the carbon star evolutionary sequence of spectral types, or alternatively developmental sequences of grain condensation in carbon-rich circumstellar shells. If decreasing temperature corresponds to increasing evolution, then decreasing temperature corresponds to increasing C/O resulting in increasing amounts of carbon rich dust, namely alpha:C-H. If decreasing the temperature corresponds to a grain condensation sequence, then heterogeneous, or induced nucleation scenarios are supported. SiC grains precede alpha:C-H and form the nuclei for the condensation of the latter material. At still lower temperatures, MgS appears to be quite prevalent. No 11.3 micron PAH features are identified in any of the 718 carbon stars. However, one of the coldest objects, IRAS 15048-5702, and a few others, displays an 11.9 micron emission feature characteristic of laboratory samples of coronene. That feature corresponds to the C-H out of plane deformation mode of aromatic hydrocarbon. This band indicates the presence of unsaturated, sp(sup 3), hydrocarbon bonds that may subsequently evolve into saturated bonds, sp(sup 2), if, and when, the star enters the planetary nebulae phase of stellar evolution. The effusion of hydrogen from the hydrocarbon grain results in the evolution in wavelength of this

  19. The 11 Micron Emissions of Cabon Stars

    NASA Technical Reports Server (NTRS)

    Goebel, J. H.; Cheeseman, P.; Gerbault, F.

    1995-01-01

    A new classification scheme of the IRAS LRS carbon stars is presented. It comprises the separation of 718 probable carbon stars into 12 distinct self-similar spectral groupings. Continuum temperatures are assigned and range from 470 to 5000 K. Three distinct dust species are identifiable: SiC, alpha:C-H, and MgS. In addition to the narrow 11 + micron emission feature that is commonly attributed to SiC, a broad 11 + micron emission feature, that is correlated with the 8.5 and 7.7 micron features, is found and attributed to alpha:C-H. SiC and alpha:C-H band strengths are found to correlate with the temperature progression among the Classes. We find a spectral sequence of Classes that reflects the carbon star evolutionary sequence of spectral types, or alternatively developmental sequences of grain condensation in carbon-rich circumstellar shells. If decreasing temperature corresponds to increasing evolution, then decreasing temperature corresponds to increasing CIO resulting in increasing amounts of carbon rich dust, namely alpha:C-H. If decreasing the temperature corresponds to a grain condensation sequence, then heterogeneous, or induced nucleation scenarios are supported. SiC grains precede alpha:C-H and form the nuclei for the condensation of the latter material. At still lower temperatures, MgS appears to be quite prevalent. No 11.3 micron PAH features are identified in any of the 718 carbon stars. However, one of the coldest objects, IRAS 15048-5702, and a few others, displays an 11.9 micron emission feature characteristic of laboratory samples of coronene. That feature corresponds to the C-H out of plane deformation mode of aromatic hydrocarbon. This band indicates the presence of unsaturated, sp(sup 3), hydrocarbon bonds that may subsequently evolve into saturated bonds, sp(sup 2), if, and when, the star enters the planetary nebulae phase of stellar evolution. The effusion of hydrogen from the hydrocarbon grain results in the evolution in wavelength of this

  20. Micron Accurate Absolute Ranging System: Range Extension

    NASA Technical Reports Server (NTRS)

    Smalley, Larry L.; Smith, Kely L.

    1999-01-01

    The purpose of this research is to investigate Fresnel diffraction as a means of obtaining absolute distance measurements with micron or greater accuracy. It is believed that such a system would prove useful to the Next Generation Space Telescope (NGST) as a non-intrusive, non-contact measuring system for use with secondary concentrator station-keeping systems. The present research attempts to validate past experiments and develop ways to apply the phenomena of Fresnel diffraction to micron accurate measurement. This report discusses past research on the phenomena, and the basis of the use Fresnel diffraction distance metrology. The apparatus used in the recent investigations, experimental procedures used, preliminary results are discussed in detail. Continued research and equipment requirements on the extension of the effective range of the Fresnel diffraction systems is also described.

  1. 3- to 13-micron spectra of Io

    NASA Technical Reports Server (NTRS)

    Noll, Keith S.; Hammel, H. B.; Young, Leslie; Joiner, Joanna; Hackwell, J.; Lynch, D. K.; Russell, R.

    1993-01-01

    The Broadband Array Spectrograph System with the NASA Infrared Telescope Facility was used to obtain 3- to 13-micron spectra of Io on June 14-16, 1991. The extinction correction and its error for each standard star (Alpha Boo, Alpha Lyr, and Mu UMa) were found individually by performing an unweighted linear fit of instrumental magnitude as a function of airmass. The model results indicate two significant trends: (1) modest differences between the two hemispheres at lower background temperatures and (2) a tendency to higher temperatures, smaller areas, and less power from the warm component at higher background temperatures with an increased contrast between the two hemispheres. The increased flux from 8 to 13 microns is due primarily to a greater area on the Loki (trailing) hemisphere for the warm component, although temperature also plays a role.

  2. 3- to 13-micron spectra of Io

    NASA Technical Reports Server (NTRS)

    Noll, Keith S.; Hammel, H. B.; Young, Leslie; Joiner, Joanna; Hackwell, J.; Lynch, D. K.; Russell, R.

    1993-01-01

    The Broadband Array Spectrograph System with the NASA Infrared Telescope Facility was used to obtain 3- to 13-micron spectra of Io on June 14-16, 1991. The extinction correction and its error for each standard star (Alpha Boo, Alpha Lyr, and Mu UMa) were found individually by performing an unweighted linear fit of instrumental magnitude as a function of airmass. The model results indicate two significant trends: (1) modest differences between the two hemispheres at lower background temperatures and (2) a tendency to higher temperatures, smaller areas, and less power from the warm component at higher background temperatures with an increased contrast between the two hemispheres. The increased flux from 8 to 13 microns is due primarily to a greater area on the Loki (trailing) hemisphere for the warm component, although temperature also plays a role.

  3. Screening for Autistic Spectrum Disorder in Children Aged 14-15 Months. II: Population Screening with the Early Screening of Autistic Traits Questionnaire (ESAT). Design and General Findings

    ERIC Educational Resources Information Center

    Dietz, Claudine; Swinkels, Sophie; van Daalen, Emma; van Engeland, Herman; Buitelaar, Jan K.

    2006-01-01

    A two-stage protocol for screening for autistic spectrum disorders (ASD) was evaluated in a random population of 31,724 children aged 14-15 months. Children were first pre-screened by physicians at well-baby clinics using a 4-item screening instrument. Infants that screened positive were then evaluated during a 1.5-h home visit by a trained…

  4. Implications of new measurements of O-16 + p + C-12,13, N-14,15 for the abundances of C, N isotopes at the cosmic ray source

    NASA Technical Reports Server (NTRS)

    Guzik, T. G.; Wefel, J. P.; Crawford, H. J.; Greiner, D. E.; Lindstrom, P. J.; Schimmerling, W.; Symons, T. J. M.

    1985-01-01

    The fragmentation of a 225 MeV/n O-16 beam was investigated at the Bevalac. Preliminary cross sections for mass = 13, 14, 15 fragments are used to constrain the nuclear excitation functions employed in galactic propagation calculations. Comparison to cosmic ray isotonic data at low energies shows that in the cosmic ray source C-13/C approximately 2% and N-14/0=3-6%. No source abundance of N-15 is required with the current experimental results.

  5. Supersonic Flows in Micron-Sized Geometries

    NASA Astrophysics Data System (ADS)

    Bayt, Robert; Breuer, Kenneth

    1998-11-01

    The results of experiments and numerical simulations of flows in micromachined converging-diverging nozzles are presented. The nozzles are fabricated using deep Reactive Ion Etching (DRIE) and are typically 20-30 microns at the throat with expansion ratios ranging from 5 to 20. The flow channels are 300 microns deep, resulting in a 10:1 or better aspect ratio at the throat. Experimental measurements of mass flow and thrust vs. pressure ratio are presented demonstrating the presence of choked and supersonic flow in the micron-scale gemoetries. Mass flow and thrust efficiencies are also presented and compared with results from two-dimensional Navier-Stokes simulations. It is found that, while the efficiencies are reasonably large (much better than one might expect, considering the small dimension of the nozzles), the boundary layers have a considerable effect, particularly on the thrust efficiency of the device, due to the relatively large displacement thickness which reduces the effective expansion ratio. The boundary layers at the top and bottom of the nozzles also affect the performance, particularly at low Reynolds numbers. Additional experimental and numerical results are also discussed.

  6. Release of Micronized Copper Particles from Pressure ...

    EPA Pesticide Factsheets

    Micronized copper pressure treated lumber (PTL) has recently been introduced to the consumer market as a replacement for ionized copper PTL. The presence of particulate rather than aqueous copper raises concerns about the exposure of humans as well as the environment to the particles. Two common pathways of exposure, leaching during contact with water and transfer during physical contact, were investigated to gage potential human and environmental risk during intended use of the product. Characterization, leaching tests, and wipe tests were conducted on two representative formulations of micronized copper PTL (micronized copper azole or MCA) to quantify the levels of copper present in the treated material and the amount of copper released during use as well as to determine the form (particle or ion) of the copper after it was released. Additionally, an ionized copper pressure treated wood (alkaline copper azole or ACA) was tested for comparison. The characterization showed that copper carbonate is the primary particle form in the MCA treated wood, but other forms are also present, particularly in the MCA-1 formulation, which contained a large amount of organically complexed copper. Microscopy showed that MCA-1 contained particles roughly half the size of MCA-2. The leaching results indicate that mostly (> ~95%) ionic copper is released from the MCA wood and that the particulate copper that was released is attached to cellulose and not free in solution. A sma

  7. Release of Micronized Copper Particles from Pressure ...

    EPA Pesticide Factsheets

    Micronized copper pressure treated lumber (PTL) has recently been introduced to the consumer market as a replacement for ionized copper PTL. The presence of particulate rather than aqueous copper raises concerns about the exposure of humans as well as the environment to the particles. Two common pathways of exposure, leaching during contact with water and transfer during physical contact, were investigated to gage potential human and environmental risk during intended use of the product. Characterization, leaching tests, and wipe tests were conducted on two representative formulations of micronized copper PTL (micronized copper azole or MCA) to quantify the levels of copper present in the treated material and the amount of copper released during use as well as to determine the form (particle or ion) of the copper after it was released. Additionally, an ionized copper pressure treated wood (alkaline copper azole or ACA) was tested for comparison. The characterization showed that copper carbonate is the primary particle form in the MCA treated wood, but other forms are also present, particularly in the MCA-1 formulation, which contained a large amount of organically complexed copper. Microscopy showed that MCA-1 contained particles roughly half the size of MCA-2. The leaching results indicate that mostly (> ~95%) ionic copper is released from the MCA wood and that the particulate copper that was released is attached to cellulose and not free in solution. A sma

  8. Identification of the origin of marker chromosomes by two-color fluorescence in situ hybridization and polymerase chain reaction in azoospermic patients.

    PubMed

    Wei, C L; Cheng, J L; Yang, W C; Li, L Y; Cheng, H C; Fu, J J

    2015-11-19

    Y chromosomal microdeletions at the azoospermia factor locus and chromosome abnormalities have been implicated as the major causes of idiopathic male infertility. A marker chromosome is a structurally abnormal chromosome in which no part can be identified by cytogenetics. In this study, to identify the origin of the marker chromosomes and to perform a genetic diagnosis of patients with azoospermia, two-color fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) techniques were carried out. The marker chromosomes for the two patients with azoospermia originated in the Y chromosome; it was ascertained that the karyotype of both patients was 46,X, ish del(Y)(q11)(DYZ3+, DXZ1-). The combination of two-color FISH and PCR techniques is an important method for the identification of the origin of marker chromosomes. Thus, genetic counseling and a clear genetic diagnosis of patients with azoospermia before intracytoplasmic sperm injection or other clinical managements are important.

  9. Current-injection two-color lasing in a wafer-bonded coupled multilayer cavity with InGaAs multiple quantum wells

    NASA Astrophysics Data System (ADS)

    Minami, Yasuo; Ota, Hiroto; Lu, Xiangmeng; Kumagai, Naoto; Kitada, Takahiro; Isu, Toshiro

    2017-04-01

    Current-injection two-color lasing has been demonstrated using a GaAs/AlGaAs coupled multilayer cavity that is a good candidate for novel terahertz-emitting devices based on difference-frequency generation (DFG) inside the structure. The coupled cavity structure was fabricated by the direct wafer bonding of (001)- and (113)B-oriented epitaxial wafers for the efficient DFG of two modes in the (113)B side cavity, and two types of InGaAs multiple quantum wells (MQWs) were introduced only in the (001) side cavity as optical gain materials. The threshold behavior was clearly observed in the current–light output curve even at room temperature. Two-color lasing was successfully observed when the gain peaks of MQWs were considerably tuned to the cavity modes by the operating temperature.

  10. Calibration of a high spatial resolution laser two-color heterodyne interferometer for density profile measurements in the TJ-II stellarator

    SciTech Connect

    Acedo, Pablo; Pedreira, P.; Criado, A. R.; Lamela, Horacio; Sanchez, Miguel; Sanchez, Joaquin

    2008-10-15

    A high spatial resolution two-color (CO{sub 2}, {lambda}=10.6 {mu}m, He-Ne, {lambda}=633 nm) interferometer for density profile measurements in the TJ-II stellarator is under development and installation, based in the currently operational single channel two-color heterodyne interferometer. To achieve the objectives of 32 channels, with 4-5 mm lateral separation between plasma chords, careful design and calibration of the interferometric waveforms for both the measurement and vibration compensation wavelengths are undertaken. The first step has been to set up in our laboratories an expanded-beam heterodyne/homodyne interferometer to evaluate the quality of both interferometric wavefronts, a reported source of poor vibration compensation and thus low resolution in the density profile measurements. This novel interferometric setup has allowed us to calibrate the spatial resolution in the profile measurements resulting in {approx}2 mm lateral resolution in the reconstruction of the interferometric wavefront.

  11. Phase control of multichannel molecular high-order harmonic generation by the asymmetric diatomic molecule HeH{sup 2+} in two-color laser fields

    SciTech Connect

    Bian Xuebin; Bandrauk, Andre D.

    2011-02-15

    Multichannel molecular high-order harmonic generation (MHOHG) from the asymmetric diatomic molecule HeH{sup 2+} in two-color laser fields is investigated from numerical simulation of the corresponding time-dependent Schroedinger equation (TDSE). It is found that the laser-induced electron transfer (LIET) plays a crucial role in MHOHG, which leads to the multichannel harmonic generation from the ground and long-lifetime excited states. LIET is sensitive to the phase differences of the two-color laser pulses, which can be used to control the enhanced excitation (EE) and enhanced ionization (EI) of the system. Both EE and EI have a strong influence on the overall intensity of the MHOHG spectrum, and there may be four orders of magnitude difference in the MHOHG intensity between the enhanced and suppressed cases. In addition, owing to the asymmetry of the two-color laser fields and the recombination of electron with the neighboring ion, multiple cutoff energies are observed. The mechanism of these effects are confirmed by classical simulations.

  12. How do we select multiple features? Transient costs for selecting two colors rather than one, persistent costs for color-location conjunctions.

    PubMed

    Lo, Shih-Yu; Holcombe, Alex O

    2014-02-01

    In a previous study Lo, Howard, & Holcombe (Vision Research 63:20-33, 2012), selecting two colors did not induce a performance cost, relative to selecting one color. For example, requiring possible report of both a green and a red target did not yield a worse performance than when both targets were green. Yet a cost of selecting multiple colors was observed when selection needed be contingent on both color and location. When selecting a red target to the left and a green target to the right, superimposing a green distractor to the left and a red distractor to the right impeded performance. Possibly, participants cannot confine attention to a color at a particular location. As a result, distractors that share the target colors disrupt attentional selection of the targets. The attempt to select the targets must then be repeated, which increases the likelihood that the trial terminates when selection is not effective, even for long trials. Consistent with this, here we find a persistent cost of selecting two colors when the conjunction of color and location is needed, but the cost is confined to short exposure durations when the observer just has to monitor red and green stimuli without the need to use the location information. These results suggest that selecting two colors is time-consuming but effective, whereas selection of simultaneous conjunctions is never entirely successful.

  13. Novel Tests of Gravity Below Fifty Microns

    NASA Astrophysics Data System (ADS)

    Martinez, Gabriela; Johnson, Jeremy; Guerrero, Ian; Hoyle, C. D.

    2016-03-01

    Due to inconsistencies between General Relativity and the Standard Model, tests of gravity remain at the forefront of experimental physics. At Humboldt State University, undergraduates and faculty are designing an experiment sensitive enough to detect gravitational interactions below the 50 micron scale. The experiment measures the twist of a torsion pendulum as an attractor mass is oscillated nearby in a parallel plate configuration, providing time varying gravitational torque on the pendulum. The size and distance dependence of the torque variation will provide a means to determine any deviation from current models of gravity on untested scales. Supported by NSF Grants 1065697 and 1306783.

  14. Predictive value of subclinical autistic traits at age 14-15 months for behavioural and cognitive problems at age 3-5 years.

    PubMed

    Möricke, Esmé; Swinkels, Sophie H N; Beuker, Karin T; Buitelaar, Jan K

    2010-08-01

    It is unclear whether subclinical autistic traits at very young age are transient or stable, and have clinical relevance. This study investigated the relationship between early subclinical autistic traits and the occurrence of later developmental and behavioural problems as well as problems in cognitive and language functioning. Parents of infants aged 14-15 months from the general population completed the Early Screening of Autistic Traits Questionnaire (ESAT). Three groups of children with high, moderate, and low ESAT-scores (total n = 103) were selected. Follow-up assessments included the CBCL 1(1/2)-5 at age 3 years, and the SCQ, the ADI-R, the ADOS-G, an on-verbal intelligence test, and language tests for comprehension and production at age 4-5 years. None of the children met criteria for autism spectrum disorder at follow-up. Children with high ESAT-scores at 14-15 months showed significantly more internalizing and externalizing problems at age 3 years and scored significantly lower on language tests at age 4-5 years than children with moderate or low ESAT-scores. Further, significantly more children with high ESAT-scores (14/26, 53.8%) than with moderate and low ESAT-scores (5/36, 13.9% and 1/41, 2.4%, respectively) were in the high-risk/clinical range on one or more outcome domains (autistic symptoms, behavioural problems, cognitive and language abilities). Subclinical autistic traits at 14-15 months predict later behavioural problems and delays in cognitive and language functioning rather than later ASD-diagnoses. The theoretical implications of the findings lie in the pivotal role of early social and communication skills for the development of self-regulation of emotions and impulses. The practical implications bear on the early recognition of children at risk for behavioural problems and for language and cognitive problems.

  15. Study of the 20,22Ne+20,22Ne and 10,12,13,14,15C+12C Fusion Reactions with MUSIC

    NASA Astrophysics Data System (ADS)

    Avila, M. L.; Rehm, K. E.; Almaraz-Calderon, S.; Carnelli, P. F. F.; DiGiovine, B.; Esbensen, H.; Hoffman, C. R.; Jiang, C. L.; Kay, B. P.; Lai, J.; Nusair, O.; Pardo, R. C.; Santiago-Gonzalez, D.; Talwar, R.; Ugalde, C.

    2016-05-01

    A highly efficient MUlti-Sampling Ionization Chamber (MUSIC) detector has been developed for measurements of fusion reactions. A study of fusion cross sections in the 10,12,13,14,15C+12C and 20,22Ne+20,22Ne systems has been performed at ATLAS. Experimental results and comparison with theoretical predictions are presented. Furthermore, results of direct measurements of the 17O(α, n)20Ne, 23Ne(α, p)26Mg and 23Ne(α, n)26Al reactions will be discussed.

  16. The 1.2 micron CMOS technology

    NASA Technical Reports Server (NTRS)

    Pina, C. A.

    1985-01-01

    A set of test structures was designed using the Jet Propulsion Laboratory (JPL) test chip assembler and was used to evaluate the first CMOS-bulk foundry runs with feature sizes of 1.2 microns. In addition to the problems associated with the physical scaling of the structures, this geometry provided an additional set of problems, since the design files had to be generated in such a way as to be capable of being processed through p-well, n-well, and twin-well processing lines. This requirement meant that the files containing the geometric design rules as well as the structure design files had to produce process-insensitive designs, a requirement that does not apply to the more mature 3.0-micron CMOS feature size technology. Because of the photolithographic steps required with this feature size, the maximum allowable chip size was 10 x 10 mm, and this chip was divided into 24 project areas, with each area being 1.6 x 1.6 mm in size. The JPL-designed structures occupied 13 out of the 21 allowable project sizes and provided the only test information obtained from these three preliminary runs. The structures were used to successfully evaluate three different manufacturing runs through two separate foundries.

  17. The Two Micron All Sky Survey

    NASA Technical Reports Server (NTRS)

    Kleinmann, S. G.; Lysaght, M. G.; Pughe, W. L.; Schneider, S. E.; Skrutskie, M. F.; Weinberg, M. D.; Price, S. D.; Matthews, K.; Soifer, B. T.; Huchra, J. P.

    1994-01-01

    The Two Micron All Sky Survey (2MASS) will provide a uniform survey of the entire sky at three near-infrared wavebands: J(lambda(sub eff) = 1.25 micrometers), H(lambda(sub eff) = 1.65 micrometers), and K(sub s)(lambda(sub eff) = 2.16 micrometers). A major goal of the survey is to probe large scale structures in the Milky Way and in the Local Universe, exploiting the relatively high transparency of the interstellar medium in the near-infrared, and the high near-infrared luminosities of evolved low- and intermediate-mass stars. A sensitive overview of the near-infrared sky is also an essential next step to maximize the gains achievable with infrared array technology. Our assessment of the astrophysical questions that might be addressed with these new arrays is currently limited by the very bright flux limit of the only preceding large scale near-infrared sky survey, the Two Micron Sky Survey carried out at Caltech in the late 1960's. Near-infrared instruments based on the new array technology have already obtained spectra of objects 1 million times fainter than the limit of the TMSS! This paper summarizes the essential parameters of the 2MASS project and the rationale behind those choices, and gives an overview of results obtained with a prototype camera that has been in operation since May 1992. We conclude with a list of expected data products and a statement of the data release policy.

  18. New MBE buffer for micron- and quarter-micron-gateGaAs MESFETs

    NASA Technical Reports Server (NTRS)

    1988-01-01

    A new buffer layer has been developed that eliminates backgating in GaAs MESFETs and substantially reduces short-channel effects in GaAs MESFETs with 0.27-micron-long gates. The new buffer is grown by molecular beam epitaxy (MBE) at a substrate temperature of 200 C using Ga and As sub 4 beam fluxes. The buffer is crystalline, highly resistive, optically inactive, and can be overgrown with high quality GaAs. GaAs MESFETs with a gate length of 0.27 microns that incorporate the new buffer show improved dc and RF properties in comparison with a similar MESFET with a thin undoped GaAs buffer. To demonstrate the backgating performance improvement afforded by the new buffer, MESFETs were fabricated using a number of different buffer layers and structures. A schematic cross section of the MESFET structure used in this study is shown. The measured gate length, gate width, and source-drain spacing of this device are 2,98, and 5.5 microns, respectively. An ohmic contact, isolated from the MESFET by mesa etching, served as the sidegate. The MESFETs were fabricated in MBE n-GaAs layers grown on the new buffer and also in MBE n-GaAs layers grown on buffer layers of undoped GaAs, AlGaAs, and GaAs/AlGaAs superlattices. All the buffer layers were grown by MBE and are 2 microns thick. The active layer is doped to approximately 2 x 10 to the 17th/cu cm with silicon and is 0.3 microns thick.

  19. New MBE buffer for micron- and quarter-micron-gateGaAs MESFETs

    NASA Technical Reports Server (NTRS)

    1988-01-01

    A new buffer layer has been developed that eliminates backgating in GaAs MESFETs and substantially reduces short-channel effects in GaAs MESFETs with 0.27-micron-long gates. The new buffer is grown by molecular beam epitaxy (MBE) at a substrate temperature of 200 C using Ga and As sub 4 beam fluxes. The buffer is crystalline, highly resistive, optically inactive, and can be overgrown with high quality GaAs. GaAs MESFETs with a gate length of 0.27 microns that incorporate the new buffer show improved dc and RF properties in comparison with a similar MESFET with a thin undoped GaAs buffer. To demonstrate the backgating performance improvement afforded by the new buffer, MESFETs were fabricated using a number of different buffer layers and structures. A schematic cross section of the MESFET structure used in this study is shown. The measured gate length, gate width, and source-drain spacing of this device are 2,98, and 5.5 microns, respectively. An ohmic contact, isolated from the MESFET by mesa etching, served as the sidegate. The MESFETs were fabricated in MBE n-GaAs layers grown on the new buffer and also in MBE n-GaAs layers grown on buffer layers of undoped GaAs, AlGaAs, and GaAs/AlGaAs superlattices. All the buffer layers were grown by MBE and are 2 microns thick. The active layer is doped to approximately 2 x 10 to the 17th/cu cm with silicon and is 0.3 microns thick.

  20. Shallow and peripheral volcanic sources of inflation revealed by modeling two-color geodimeter and leveling data from Long Valley caldera, California, 1988-1992

    SciTech Connect

    Langbein, J.; Dzurisin, D.; Marshall, G.

    1995-07-10

    The authors refined the model for inflation of the Long Valley caldera near Mammoth Lakes, California, by combining both geodetic measurements of baseline length and elevation changes. Baseline length changes measured using a two-color geodimeter with submillimeter precision revealed that the resurgent dome started to reinflate in late 1989. Measurements between late 1989 and mid-1992 revealed nearly 13 cm of extension across the resurgent dome. Geodetic leveling surveys with approximately 2-mm precision made in late 1988 and in mid-1992 revealed a maximum of about 8 cm of uplift of the resurgent dome. Two elliposidal sources satisfy both the leveling and two-color measurements, whereas spherical point sources could not. The model`s primary inflation source is located 5.5 km beneath the resurgent dome with the two horizontal axes. A second source was added to improve the fit to the two-color measurements. This secondary source is located at a depth between 10 and 20 km beneath the south moat of the caldera and has the geometry of an elongated ellipsoid or pipe that dips down to the northeast. In addition, the leveling data suggest dike intrusion beneath Mammoth Mountain during the 1988-1992 interval, which is likely associated with an intense swarm of small earthquakes during the summer of 1989 at that location. The authors analysis shows the dike intrusion to be the shallowest of the three sources with a depth range of 1-3 km below the surface to the top of the intrusion. 18 refs., 4 figs., 2 tabs.

  1. Soot volume fractions and primary particle size estimate by means of the simultaneous two-color-time-resolved and 2D laser-induced incandescence

    NASA Astrophysics Data System (ADS)

    Boiarciuc, A.; Foucher, F.; Mounaïm-Rousselle, C.

    2006-06-01

    An original approach of laser-induced incandescence consisting in the simultaneous recording of the two-color-time-resolved and 2D LII signal is described in this paper. The application of this approach in an atmospheric pressure diffusion flame fueled with isooctane as well as inside the combustion chamber of a diesel engine is presented. Soot volume fraction and primary particle diameters are calculated, and the results are discussed. The mean diameter estimated by fitting the LII modeled curve on the experimental one is compared with the results obtained through soot sampling and microscope analyzing. The influence of the thermal accommodation coefficient and soot refractive index function is also discussed.

  2. Two-color GaN/AlGaN quantum cascade detector at short infrared wavelengths of 1 and 1.7 μm

    NASA Astrophysics Data System (ADS)

    Sakr, S.; Giraud, E.; Dussaigne, A.; Tchernycheva, M.; Grandjean, N.; Julien, F. H.

    2012-04-01

    A two-color GaN-based quantum cascade detector is demonstrated. This photodetector operates simultaneously at a peak wavelength of 1.7 and 1 μm at room temperature without any external voltage. These peaks correspond, respectively, to the e1e2 and e1e3 intersubband absorption of the active GaN quantum well. The extractor has been designed to allow for efficient transfer of electrons from both the e2 and e3 states to the next period. The 1 μm detected wavelength is the shortest value reported for an intersubband semiconductor based detector.

  3. Radiation tolerant 1 micron CMOS technology

    NASA Astrophysics Data System (ADS)

    Crevel, P.; Rodde, K.

    1991-03-01

    Starting from a standard one micron Complementary Metal Oxide Semiconductor (CMOS) for high density, low power memory applications, the degree of radiation tolerance of the baseline process is evaluated. Implemented process modifications to improve latchup sensitivity under heavy ion irradiation as well as total dose effects without changing layout rules are described. By changing doping profiles in Metal Nitride Oxide Semiconductors (MNOS) and P-channel MOS (PMOS) device regions, it is possible to guarantee data sheet specification of a 64 K low power static RAM for total gamma dose up to 35 krad (Si) (and even higher values for the gate array family) without latch up for Linear Energy Transfer LET up to 115 MeV/(mg/cm squared).

  4. Novel Tests of Gravity Below Fifty Microns

    NASA Astrophysics Data System (ADS)

    Johnson, Jeremy; Martinez, Gabriela; Guerrero, Ian; Dunkley, Noah; Sanchez, Anthony; Isachsen, Hilde; Shaw, Duncan; Hoyle, C. D.

    2017-01-01

    Theories which attempt to unify the Standard Model and General Relativity often include features which violate the Weak Equivalence Principle (WEP) and gravitational Inverse-Square Law (ISL). A violation of either the WEP or ISL at any length scale would bring into question our fundamental understanding of gravity. Motivated by these considerations, undergraduates and faculty at Humboldt State University are building an experiment to probe gravitational interactions below the 50-micron length scale. The experiment employs a torsion pendulum with equal masses of different material arranged as a ``composition dipole.'' We measure the twist of the torsion pendulum as an attractor mass is oscillated nearby in a parallel-plate configuration, providing a time varying torque on the pendulum. The size and distance dependence of the torque variation will provide a means to determine any deviation from the WEP or ISL at untested scales. PHY-1065697, PHY-1306783, and PHY-1606988.

  5. 20-micron observations of Cassiopeia A

    NASA Astrophysics Data System (ADS)

    Greidanus, H.; Strom, R. G.

    1991-09-01

    Ground-based observations of a small region on the northern rim of Cas A showing structures down to the resolution limit of 5 sec are discussed. It is shown that the brightness distribution in the area has no correlation with the optical, radio, or X-ray emission. The radiating mass derived is found to be comparable to earlier infrared-based estimates, but substantially smaller than X-ray-derived values; this indicates a very low dust-to-gas ratio or coupling, or a very low filling factor for the hot gas. A detailed comparison between 20-micron and X-ray brightness distributions indicate that the dust is associated with the hottest gas found in the blast wave. This is found to be in contrast with its relative inward location near the cooler gas, that can be interpreted as reverse shocked ejecta.

  6. Micronized progesterone reduces vasoconstriction in the placenta.

    PubMed

    Miller, Nathaniel R; Dolinsky, Brad M; Napolitano, Peter G

    2015-09-01

    To investigate if micronized progesterone (P4) has the ability to attenuate thromboxane mimetic U46619-mediated fetoplacental artery vasoconstriction. Paired cotyledons obtained from the same placenta of five-term subjects were analyzed. The fetal artery and maternal intervillous space of one cotyledon was infused with P4 while another cotyledon was infused with control perfusate. After 30 min, a bolus dose of U44619 was administered to both cotyledons. The change in the measured fetoplacental vascular pressure caused by bolus administration of U46619 was less in the cotyledons infused with P4 (p = 0.009). Continuous treatment with P4 significantly attenuates the U46619-mediated fetoplacental vasoconstriction.

  7. Two-color In0.4Ga0.6As/Al0.1Ga0.9As quantum dot infrared photodetector with double tunneling barriers

    NASA Astrophysics Data System (ADS)

    Huang, Jianliang; Ma, Wenquan; Wei, Yang; Zhang, Yanhua; Huo, Yongheng; Cui, Kai; Chen, Lianghui

    2011-03-01

    We report a two-color quantum dot infrared photodetector (QDIP) using double tunneling barriers (DTBs) on one side of the InGaAs/AlGaAs dots. The two-color detection is achieved by changing the polarity of the applied bias voltages. In contrast, the same QDIP structure without the DTBs does not exhibit this detection wavelength tunability by switching the bias polarity. The two-color detection is ascribed to a different escape mechanism of electrons between positive and negative biases. The electrons escape out of the quantum well through resonant tunneling for a positive bias voltage while tunnel through a triangular barrier for a negative bias voltage.

  8. Lunar multispectral imaging at 2.26 microns - First results

    NASA Technical Reports Server (NTRS)

    Davies, D. W.; Johnson, T. V.; Matson, D. L.

    1979-01-01

    The first results of line-scan imaging of the moon at 2.26 microns and 0.56 micron are presented. Among the many features observed in the 2.26 micron/0.56 micron ratio image, fresh rock and immature soils stand out as dark (i.e., low ratio) due to their infrared absorption bands. Also notable in this image are several strikingly bright (high 2.26 micron/0.56 micron ratio) areas which are likely to contain pyroclastic, glass-related materials. Some of these bright areas correspond to dark mantling material. Others (e.g., Sulpicius Gallus, Aristarchus Plateau) are distinctly different in their overall spectral reflectance and it is suggested that these localities contain varieties of pyroclastic glass-related materials which were not sampled by Apollo 17 but which are relatively abundant elsewhere on the moon.

  9. Evaluation of selected micronized poloxamers as tablet lubricants.

    PubMed

    Desai, D; Zia, H; Quadir, A

    2007-10-01

    The primary objective of this study was to compare the lubrication properties of micronized poloxamer 188 (Lmicrotrol micro 68) and micronized poloxamer 407 (Lmicrotrol micro 127) with certain conventional lubricants such as magnesium stearate and stearic acid. The secondary objective was to use these micronized poloxamers as water-soluble tablet lubricants in preparation of effervecsent tablets. The results showed that these micronized poloxamers have superior lubrication properties compared with stearic acid, with no negative effect on tablet hardness, friability, disintegration, or dissolution. Moreover, lubricant mixing time had no significant effect on tablet properties when poloxamers were used as lubricants. Effervescent tablets also were produced successfully using micronized poloxamers as lubricants. The micronized poloxamers had a better lubrication effect in comparison with that of water-soluble lubricant l-leucine.

  10. 3 micron spectrophotometry of Comet Halley - Evidence for water ice

    NASA Technical Reports Server (NTRS)

    Bregman, Jesse D.; Tielens, A. G. G. M.; Witteborn, Fred C.; Rank, David M.; Wooden, Diane

    1988-01-01

    Structure has been observed in the 3-3.6 micron preperihelion spectrum of Comet Halley consistent with either an absorption band near 3.1 microns or emission near 3.3 microns. The results suggest that a large fraction of the water molecules lost by the comet are initially ejected in the form of small ice particles rather than in the gas phase.

  11. Investigation on thermal accommodation coefficient and soot absorption function with two-color Tire-LII technique in rich premixed flames

    NASA Astrophysics Data System (ADS)

    Maffi, S.; De Iuliis, S.; Cignoli, F.; Zizak, G.

    2011-08-01

    Although the two-color laser-induced incandescence technique (2C-LII) has proved to be a significant tool for soot diagnostics, many efforts are still required to gain a whole understanding of the chemical and physical processes involved. Time-resolved two-color LII measurements are carried out in a rich ethylene/air premixed flame at different heights above the burner and by changing the laser fluence. The prompt LII at two wavelengths and the corresponding soot incandescence temperature are obtained at different stages of the soot growth and under different laser irradiations. The decay rate of the LII signals, as a method for soot sizing, is investigated at different laser fluence. The time-resolved LII curves, obtained in the low laser fluence regime, are analyzed by a numerical simulation, available on the web. By considering the gas/particle initial temperature obtained with thermocouple measurements and by knowing soot particle diameter with previous TEM and extinction/scattering measurements, information about soot parameters, such as absorption function and thermal accommodation coefficient are obtained. The presence of the so-called young or mature soot along the flame height is strictly related to different optical and heat-exchange properties necessary to fit all the experimental data available.

  12. The spatial distribution in high-order harmonic generation of H2+ with different time delays of the two-color laser fields

    NASA Astrophysics Data System (ADS)

    Zhang, Jun; Pan, Xue-Fei; Du, Hui; Xu, Tong-Tong; Guo, Jing; Liu, Xue-Shen

    2017-01-01

    We theoretically investigate the high-order harmonic generation (HHG) and the spatial distribution in HHG of the H2+ molecule by introducing a time-delayed two-color laser field which consists of the mid-infrared and near-infrared laser field. For the different time delays of the two-color laser fields, the pulse shapes are changed which result in the variation of the electron recombined with the nuclei along the positive- or negative-z direction. When the time delay is 0 fs (1.34 fs), a smooth harmonic plateau from the electron recombined with the nuclei along the negative (positive)-z direction can be achieved. An isolated attosecond pulse with a duration of about 99 as is generated when the time delay is 1.34 fs. We perform the classical analysis which is consistent with the numerical results from the one-dimensional non-Born-Oppenheimer time dependent Schrödinger equation (TDSE). We also investigate emission time of harmonics in terms of a time-frequency analysis to further understand the underlying physical mechanism.

  13. Simultaneous two-color stimulated Raman scattering microscopy by adding a fiber amplifier to a 2 ps OPO-based SRS microscope.

    PubMed

    Yang, Wenlong; Li, Ang; Suo, Yuanzhen; Lu, Fa-Ke; Sunney Xie, X

    2017-02-01

    Stimulated Raman scattering (SRS) microscopy is a label-free chemical imaging technique. Two-color imaging is often necessary to determine the distribution of chemical species in SRS microscopy. Current multi-color SRS imaging methods involve complicated instrumentation or longer data acquisition time or are limited to transmission imaging. In this Letter, we show that by adding a simple fiber amplifier to a 2 ps laser source and optical-parametric-oscillator-based SRS setup, one can achieve simultaneous two-color or frequency modulation SRS microscopy. The fiber amplifier can generate a wavelength tunable laser of ±10  nm around the Stokes laser wavelength at 1031 nm with average power greater than 200 mW. In vivo and ex vivo lipid-protein imaging of mouse brain and skin is demonstrated. To further demonstrate the potential of this technique in high-speed in vivo imaging, white blood cells in a blood stream are imaged in a live mouse.

  14. 128 × 128 long-wavelength/mid-wavelength two-color HgCdTe infrared focal plane array detector with ultralow spectral cross talk.

    PubMed

    Hu, Weida; Ye, Zhenhua; Liao, Lei; Chen, Honglei; Chen, Lu; Ding, Ruijun; He, Li; Chen, Xiaoshuang; Lu, Wei

    2014-09-01

    High temporal and spatial coherent simultaneous long-wavelength/mid-wavelength (LW/MW) two-color focal plane array (FPA) infrared detection is the cutting-edge technique for third-generation infrared remote sensing. In this Letter, HgCdTe LW/MW two-color infrared detectors were designed and fabricated. The top long-wavelength and bottom mid-wavelength infrared planar photodiodes were processed by selective B(+)-implantation after etching the long-wavelength epilayer into a curvature and exposing the mid-wavelength layers for the implantation of the n region of the MW photodiode by a micro-mesa array technique. A 128×128  MW/LW HgCdTe infrared FPA detector is fabricated photo-lithographically by simultaneous nonplanar B(+)-implantation of the LW and MW photodiodes, passivation and metallization of the sidewalls, mesa isolation, and flip-chip hybridization with a read-out integrated circuit. The inner mechanisms for suppressing the cross talk and improving photoresponse have been carried out by combining experimental work with numerical simulations.

  15. Near 16 micron CO.sub.2 laser system

    DOEpatents

    Krupke, William F.

    1977-01-01

    Method and apparatus for inducing laser action in CO.sub.2 at a wavelength of 16 microns involving the transition between the 02.sup.0 0 and 01.sup.1 0 states. The population inversion between these two states is achieved by pumping to the 00.sup.0 1 level, suppressing the usual 10.6 micron transition to the 10.sup.0 0 level and encouraging the 9.6 micron transition, thereby populating the 02.sup.0 0 level, as the principal prerequisite for 16 micron laser action between the 02.sup.0 0 and 01.sup.1 0 levels.

  16. Roles of the 2 microns gene products in stable maintenance of the 2 microns plasmid of Saccharomyces cerevisiae.

    PubMed Central

    Reynolds, A E; Murray, A W; Szostak, J W

    1987-01-01

    We have examined the replication and segregation of the Saccharomyces cerevisiae 2 microns circle. The amplification of the plasmid at low copy numbers requires site-specific recombination between the 2 microns inverted repeat sequences catalyzed by the plasmid-encoded FLP gene. No other 2 microns gene products are required. The overexpression of FLP in a strain carrying endogenous 2 microns leads to uncontrolled plasmid replication, longer cell cycles, and cell death. Two different assays show that the level of Flp activity decreases with increasing 2 microns copy number. This regulation requires the products of the REP1 and REP2 genes. These gene products also act together to ensure that 2 microns molecules are randomly segregated between mother and daughter cells at cell division. Images PMID:3316982

  17. Tuberculin skin test negativity is under tight genetic control of chromosomal region 11p14-15 in settings with different tuberculosis endemicities.

    PubMed

    Cobat, Aurélie; Poirier, Christine; Hoal, Eileen; Boland-Auge, Anne; de La Rocque, France; Corrard, François; Grange, Ghislain; Migaud, Mélanie; Bustamante, Jacinta; Boisson-Dupuis, Stéphanie; Casanova, Jean-Laurent; Schurr, Erwin; Alcaïs, Alexandre; Delacourt, Christophe; Abel, Laurent

    2015-01-15

    A substantial proportion of subjects exposed to a contagious tuberculosis case display lack of tuberculin skin test (TST) reactivity. We previously mapped a major locus (TST1) controlling lack of TST reactivity in families from an area in South Africa where tuberculosis is hyperendemic. Here, we conducted a household tuberculosis contact study in a French area where the endemicity of tuberculosis is low. A genome-wide analysis of TST negativity identified a significant linkage signal (P < 3 × 10(-5)) in close vicinity of TST1. Combined analysis of the 2 samples increased evidence of linkage (P = 2.4 × 10(-6)), further implicating genetic factors located on 11p14-15. This region overlaps the TNF1 locus controlling mycobacteria-driven tumor necrosis factor α production. © The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.

  18. Investigation of the pairing effect using newly evaluated empirical studies for 14-15 MeV neutron reaction cross sections

    SciTech Connect

    Tel, E.; Tanir, G.; Aydin, A.

    2007-03-15

    The asymmetry term effects for the cross sections of (n, charged particle) and (n,2n) reactions at 14-15 MeV neutron incident energy have been investigated. The effects of pairing and odd-even nucleon numbers in new data and in the formula of Tel et al. [J. Phys. G. 29, 2169 (2003)] are discussed. We have determined three different parameters groups by the classification of nuclei into even-even, even-odd, and odd-even (n,d) reactions. In addition, since there are not enough experimental data available, we have considered two different parameters groups by the classification of nuclei into odd-A and even-A (n,t) reaction cross sections. The empirical formulas with two parameters for the evaluation of the (n,d) and (n,t) reactions cross sections are discussed in the present study.

  19. Sub-micron geochronology by EPMA

    NASA Astrophysics Data System (ADS)

    Jercinovic, M. J.; Williams, M. L.; Berman, R.

    2013-12-01

    Actinide-bearing, geochronologically applicable accessory phases can exhibit remarkably complex internal compositional structures, commonly revealing polygenesis and allowing timing constraints to be placed on the structural and metamorphic evolution of multiply tectonized terrains. These fine-scale mineral domains have been revealed by high resolution compositional mapping by EPMA of natural monazite (LREE PO4), and can exist at nearly all spatial scales, including domains substantially below 1 micron in width. Accurate compositional characterization of such domains by any technique is difficult, but hardware and software developments in EPMA offer some opportunities to approach these challenging but important targets accurately. In this regard, we have analyzed Paloeproterozoic (ca. 1.8 Ga) monazite domains which penetrate along cleavage planes in larger, older monazite grains (ca. 2.55 and 2.37 Ga) from the Boothia peninsula, Nanavut Canada. As such, this represents a successful nanogeochronologic analysis. The direct analytical volume, as defined by the convolution of the error functions resulting from primary (beam) electron scattering and characteristic X-ray generation volume dimensions, can be restricted to considerably less than 1 micron with the use of high brightness sources (LaB6, CeB6, or Schottky) and modest beam energy (15kV or less) in monazite (Z ca. 39). However, the analysis is rendered far more complex by the effects of boundary fluorescence and beam damage. For this analysis, we use a high thermal/electrical conductivity double coating of aluminum and carbon to mitigate sample damage at high current density. Although imperfect (there remains a net loss of P relative to REEs and actinides), this analysis results in relatively minor counting errors relative to the concentrations, and accuracy can be further improved by use of time-dependent count acquisition methods. Reduction of the beam energy substantially below 10kV results in extreme damage

  20. Deformation Behavior of Sub-micron and Micron Sized Alumina Particles in Compression.

    SciTech Connect

    Sarobol, Pylin; Chandross, Michael E.; Carroll, Jay; Mook, William; Boyce, Brad; Kotula, Paul Gabriel; McKenzie, Bonnie Beth; Bufford, Daniel Charles; Hall, Aaron Christopher.

    2014-09-01

    The ability to integrate ceramics with other materials has been limited due to high temperature (>800degC) ceramic processing. Recently, researchers demonstrated a novel process , aerosol deposition (AD), to fabricate ceramic films at room temperature (RT). In this process, sub - micro n sized ceramic particles are accelerated by pressurized gas, impacted on the substrate, plastically deformed, and form a dense film under vacuum. This AD process eliminates high temperature processing thereby enabling new coatings and device integration, in which ceramics can be deposited on metals, plastics, and glass. However, k nowledge in fundamental mechanisms for ceramic particle s to deform and form a dense ceramic film is still needed and is essential in advancing this novel RT technology. In this wo rk, a combination of experimentation and atomistic simulation was used to determine the deformation behavior of sub - micron sized ceramic particle s ; this is the first fundamental step needed to explain coating formation in the AD process . High purity, singl e crystal, alpha alumina particles with nominal size s of 0.3 um and 3.0 um were examined. Particle characterization, using transmission electron microscopy (TEM ), showed that the 0.3 u m particles were relatively defect - free single crystals whereas 3.0 u m p articles were highly defective single crystals or particles contained low angle grain boundaries. Sub - micron sized Al 2 O 3 particles exhibited ductile failure in compression. In situ compression experiments showed 0.3um particles deformed plastically, fractured, and became polycrystalline. Moreover, dislocation activit y was observed within the se particles during compression . These sub - micron sized Al 2 O 3 particles exhibited large accum ulated strain (2 - 3 times those of micron - sized particles) before first fracture. I n agreement with the findings from experimentation , a tomistic simulation s of nano - Al 2 O 3 particles showed dislocation slip and

  1. Millisecond, micron precision multi-whisker detector.

    PubMed

    Grady, Stephen K; Hoang, Thanh T; Gautam, Shree Hari; Shew, Woodrow L

    2013-01-01

    The neural mechanisms of somatosensory information processing in the rodent vibrissae system are a topic of intense debate and research. Certain hypotheses emphasize the importance of stick-slip whisker motion, high-frequency resonant vibrations, and/or the ability to decode complex textures. Other hypotheses focus on the importance of integrating information from multiple whiskers. Tests of the former require measurements of whisker motion that achieve high spatiotemporal accuracy without altering the mechanical properties of whiskers. Tests of the latter require the ability to monitor the motion of multiple whiskers simultaneously. Here we present a device that achieves both these requirements for two-dimensional whisker motion in the plane perpendicular to the whiskers. Moreover, the system we present is significantly less expensive (<$2.5 k) and simpler to build than alternative devices which achieve similar detection capabilities. Our system is based on two laser diodes and two linear cameras. It attains millisecond temporal precision and micron spatial resolution. We developed automated algorithms for processing the data collected by our device and benchmarked their performance against manual detection by human visual inspection. By this measure, our detection was successful with less than 10 µm deviation between the automated and manual detection, on average. Here, we demonstrate its utility in anesthetized rats by measuring the motion of multiple whiskers in response to an air puff.

  2. Emergence of electron coherence and two-color all-optical switching in MoS2 based on spatial self-phase modulation

    PubMed Central

    Wu, Yanling; Wu, Qiong; Sun, Fei; Cheng, Cai; Meng, Sheng; Zhao, Jimin

    2015-01-01

    Generating electron coherence in quantum materials is essential in optimal control of many-body interactions and correlations. In a multidomain system this signifies nonlocal coherence and emergence of collective phenomena, particularly in layered 2D quantum materials possessing novel electronic structures and high carrier mobilities. Here we report nonlocal ac electron coherence induced in dispersed MoS2 flake domains, using coherent spatial self-phase modulation (SSPM). The gap-dependent nonlinear dielectric susceptibility χ(3) measured is surprisingly large, where direct interband transition and two-photon SSPM are responsible for excitations above and below the bandgap, respectively. A wind-chime model is proposed to account for the emergence of the ac electron coherence. Furthermore, all-optical switching is achieved based on SSPM, especially with two-color intraband coherence, demonstrating that electron coherence generation is a ubiquitous property of layered quantum materials. PMID:26351696

  3. Polarization dependence of absorption in strongly vertically coupled InAs/GaAs quantum dots for two-color far-infrared photodetector

    NASA Astrophysics Data System (ADS)

    Wang, Zhicheng; Chen, Yonghai; Xu, Bo; Liu, Fengqi; Shi, Liwei; Tang, Chenguang; Wang, Zhanguo

    2008-01-01

    Strongly vertically coupled InAs/GaAs quantum dots (QDs) with modulation doping are investigated, and polarization dependence of two-color absorptions was observed. Analysis of photoluminescence (PL) and absorption spectra shows that s-polarized absorptions at 10.0 and 13.4 μm stem from the first excited state E1 and the second excited state E2 in the QDs to the bound state EInGaAs in the InGaAs spacer, respectively, whereas p-polarized absorptions at 10.0 and 8.2 μm stem from the first excited state E1 and the ground Eg in the QDs to the bound state EInGaAs in the InGaAs spacer, respectively. These measurements illustrate that transitions from excited states are more sensitive to normal incidence, which are very important in designing QD infrared detector.

  4. Origin of ellipticity of high-order harmonics generated by a two-color laser field in the cross-polarized configuration

    NASA Astrophysics Data System (ADS)

    Stremoukhov, S.; Andreev, A.; Vodungbo, B.; Salières, P.; Mahieu, B.; Lambert, G.

    2016-07-01

    Recently several techniques demonstrated the production of elliptically polarized high harmonics. One of these techniques consists of the interaction in a noble gas of two-color laser beams having orthogonal linear polarizations. Here we present the theoretical explanation of such a result observed in Lambert et al. [Nat. Commun. 6, 6167 (2015), 10.1038/ncomms7167]. Numerical calculations based on the nonperturbative light-atom interaction theory reproduce well the experimental data. The degree of polarization is analyzed for different harmonic orders and found to be high. With the help of a simplified theoretical model it is shown that the degree of harmonic ellipticity depends mainly on the population of atomic state sublevels with different angular momentum projections.

  5. Two-color spectroscopy of UV excited ssDNA complex with a single-wall nanotube (SWNT) probe: Fast nucleobase autoionization mechanism

    NASA Astrophysics Data System (ADS)

    Rotkin, Slava V.; Ignatova, Tetyana; Balaeff, Alexander; Zheng, Ming; Blades, Michael; Stoeckl, Peter

    DNA autoionization is a fundamental process wherein UV-photoexcited nucleobases dissipate energy to the environment without undergoing chemical damage. SWNT is shown to serve as a photoluminescent reporter for studying the mechanism and rates of DNA autoionization. Two-color photoluminescence (PL) spectroscopy revealed a strong SWNT PL quenching when the UV pump is resonant with the DNA absorption [Nano Research, 2015]. Semiempirical calculations of the DNA-SWNT electronic structure, combined with a Green's function theory for charge transfer, show a 20 fs autoionization rate, dominated by the hole transfer. Rate-equation analysis of the spectroscopy data confirms that the quenching rate is limited by the thermalization of the free charge carriers transferred to the nanotube reservoir. The developed approach has a great potential for monitoring DNA excitation, autoionization, and chemical damage both in vivo and in vitro. NSF ECCS-1509786 (S.V.R.,T.I.) and PHY-1359195 (P.S.), NIST and UCF facilities.

  6. Electronic spectra of radicals in a supersonic slit-jet discharge by degenerate and two-color four-wave mixing.

    PubMed

    Mazzotti, Fabio J; Achkasova, Elena; Chauhan, Richa; Tulej, Marek; Radi, Peter P; Maier, John P

    2008-01-07

    Four-wave mixing techniques have been used for the measurement of electronic transitions of cold transient species generated in a supersonic slit-jet discharge expansion. The origin band of the d(3)Pi(g)-a(3)Pi(u) system of C(2) and A(2)Pi(3/2)-X[combining tilde](2)Pi(3/2) electronic transition of HC(4)S were recorded. A signal-to-noise ratio of 10(4) in the spectra was achieved, resulting in detection limits of 10(10) cm(-3) for these two molecules. Application of selective two-color resonant four-wave mixing is used for the spectral assignment utilizing the double-resonance nature of the method. The combination of these techniques with a slit source proves to be a sensitive approach for the detection of transient molecules in a molecular beam discharge.

  7. Selective detection of radicals and ions in a slit-jet discharge by degenerate and two-color four-wave mixing.

    PubMed

    Raghunandan, Ranjini; Mazzotti, Fabio J; Chauhan, Richa; Tulej, Marek; Maier, John P

    2009-11-26

    Degenerate four-wave mixing (DFWM) was used to record the spectra of charged and neutral carbon-containing radicals generated in a pulsed discharge source within a supersonic slit-jet expansion. Detection limits of approximately 10(9) molecules cm(-3) are achieved. The DFWM method allows a selective molecular detection by varying the discharge timings. Increased spectral selectivity is obtained by applying the two-color, doubly resonant four-wave mixing variant. This shows the potential of the techniques for sensitive and selective spectral analysis of radicals in discharges. The methods are successfully used for the detection of C(4)H, HC(2)S, and HC(4)H(+) with signal-to-noise in the range of 10(2)-10(4).

  8. Generation of Bright, Spatially Coherent Soft X-Ray High Harmonics in a Hollow Waveguide Using Two-Color Synthesized Laser Pulses.

    PubMed

    Jin, Cheng; Stein, Gregory J; Hong, Kyung-Han; Lin, C D

    2015-07-24

    We investigate the efficient generation of low-divergence high-order harmonics driven by waveform-optimized laser pulses in a gas-filled hollow waveguide. The drive waveform is obtained by synthesizing two-color laser pulses, optimized such that highest harmonic yields are emitted from each atom. Optimization of the gas pressure and waveguide configuration has enabled us to produce bright and spatially coherent harmonics extending from the extreme ultraviolet to soft x rays. Our study on the interplay among waveguide mode, atomic dispersion, and plasma effect uncovers how dynamic phase matching is accomplished and how an optimized waveform is maintained when optimal waveguide parameters (radius and length) and gas pressure are identified. Our analysis should help laboratory development in the generation of high-flux bright coherent soft x rays as tabletop light sources for applications.

  9. Two-color SERS microscopy for protein co-localization in prostate tissue with primary antibody-protein A/G-gold nanocluster conjugates.

    PubMed

    Salehi, Mohammad; Schneider, Lilli; Ströbel, Philipp; Marx, Alexander; Packeisen, Jens; Schlücker, Sebastian

    2014-02-21

    SERS microscopy is a novel staining technique in immunohistochemistry, which is based on antibodies labeled with functionalized noble metal colloids called SERS labels or nanotags for optical detection. Conventional covalent bioconjugation of these SERS labels cannot prevent blocking of the antigen recognition sites of the antibody. We present a rational chemical design for SERS label-antibody conjugates which addresses this issue. Highly sensitive, silica-coated gold nanoparticle clusters as SERS labels are non-covalently conjugated to primary antibodies by using the chimeric protein A/G, which selectively recognizes the Fc part of antibodies and therefore prevents blocking of the antigen recognition sites. In proof-of-concept two-color imaging experiments for the co-localization of p63 and PSA on non-neoplastic prostate tissue FFPE specimens, we demonstrate the specificity and signal brightness of these rationally designed primary antibody-protein A/G-gold nanocluster conjugates.

  10. A single isolated sub-50 attosecond pulse generation with a two-color laser field by a frequency-chirping technique

    NASA Astrophysics Data System (ADS)

    Zhao, Kun; Chu, Tianshu

    2011-07-01

    We discuss the possibility of using the frequency-chirping technique to shorten the duration of the generated single attosecond pulse (SAP) by a two-color laser field of 800 and 1600 nm with few-cycle pulses. By adopting various combinations of the two frequency-chirped laser fields in our numerical simulation of ionizing He atom, we demonstrate that the best possible condition to obtain the shortest SAP is using the same chirping in both the fundamental and the half-harmonic laser fields without any phase effect and any delay time. There is a maximum increment of about 40 eV in the bandwidth of the XUV super-continuum in the cutoff (the second plateau) region. A single isolated attosecond pulse of 48 as can be generated that is further reduced to 9.7 as by phase compensation.

  11. Generation of Bright, Spatially Coherent Soft X-Ray High Harmonics in a Hollow Waveguide Using Two-Color Synthesized Laser Pulses

    NASA Astrophysics Data System (ADS)

    Jin, Cheng; Stein, Gregory J.; Hong, Kyung-Han; Lin, C. D.

    2015-07-01

    We investigate the efficient generation of low-divergence high-order harmonics driven by waveform-optimized laser pulses in a gas-filled hollow waveguide. The drive waveform is obtained by synthesizing two-color laser pulses, optimized such that highest harmonic yields are emitted from each atom. Optimization of the gas pressure and waveguide configuration has enabled us to produce bright and spatially coherent harmonics extending from the extreme ultraviolet to soft x rays. Our study on the interplay among waveguide mode, atomic dispersion, and plasma effect uncovers how dynamic phase matching is accomplished and how an optimized waveform is maintained when optimal waveguide parameters (radius and length) and gas pressure are identified. Our analysis should help laboratory development in the generation of high-flux bright coherent soft x rays as tabletop light sources for applications.

  12. A biological question and a balanced (orthogonal) design: the ingredients to efficiently analyze two-color microarrays with Confirmatory Factor Analysis

    PubMed Central

    Crijns, Anne PG; Gerbens, Frans; Plantinga, A Edo D; Meersma, Gert Jan; de Jong, Steven; Hofstra, Robert MW; de Vries, Elisabeth GE; van der Zee, Ate GJ; de Bock, Geertruida H; te Meerman, Gerard J

    2006-01-01

    Background Factor analysis (FA) has been widely applied in microarray studies as a data-reduction-tool without any a-priori assumption regarding associations between observed data and latent structure (Exploratory Factor Analysis). A disadvantage is that the representation of data in a reduced set of dimensions can be difficult to interpret, as biological contrasts do not necessarily coincide with single dimensions. However, FA can also be applied as an instrument to confirm what is expected on the basis of pre-established hypotheses (Confirmatory Factor Analysis, CFA). We show that with a hypothesis incorporated in a balanced (orthogonal) design, including 'SelfSelf' hybridizations, dye swaps and independent replications, FA can be used to identify the latent factors underlying the correlation structure among the observed two-color microarray data. An orthogonal design will reflect the principal components associated with each experimental factor. We applied CFA to a microarray study performed to investigate cisplatin resistance in four ovarian cancer cell lines, which only differ in their degree of cisplatin resistance. Results Two latent factors, coinciding with principal components, representing the differences in cisplatin resistance between the four ovarian cancer cell lines were easily identified. From these two factors 315 genes associated with cisplatin resistance were selected, 199 genes from the first factor (False Discovery Rate (FDR): 19%) and 152 (FDR: 24%) from the second factor, while both gene sets shared 36. The differential expression of 16 genes was validated with reverse transcription-polymerase chain reaction. Conclusion Our results show that FA is an efficient method to analyze two-color microarray data provided that there is a pre-defined hypothesis reflected in an orthogonal design. PMID:16968534

  13. A rapid technique for lymphocyte preparation prior to two-color immunofluorescence analysis of lymphocyte subsets using flow cytometry. Comparison with density gradient separation.

    PubMed

    Mansour, I; Bourin, P; Rouger, P; Doinel, C

    1990-02-20

    A technique is described for lymphocyte preparation which permits analyses by two-color immunofluorescence and flow cytometry. It consists, briefly, of the lysis of red blood cells and washing of white blood cells prior to labeling. We tested this technique with a large panel of monoclonal antibodies in mono- and dual immunofluorescence. By comparing these results to those obtained after density gradient separation, we found the following statistically significant differences: the count of the phenotype B1+ was higher after whole blood lysis preparation than after density gradient separation; whereas, the corresponding counts of OKT4+ and Leu-4-Leu-7+ phenotypes were lower. No difference was detected with OKT8+, Leu-4+, OKT8+Leu-4+, OKT8+Leu-4-, OKT8-Leu-4+, OKT8+Leu-7+, Leu-4+Leu-7+, Leu-4-Leu-11c+, OKT8+Leu-11c+ and OKT8+Leu-15+ phenotypes. We have studied the reproducibility of both methods and the correlation between them. The disparity of the lymphocyte subset count between these two methods, though statistically significant, was relatively weak and seems to be due to the density gradient separation. Since the preparation of lymphocytes using the density gradient method is time consuming, we propose whole blood lysis as an alternative lymphocyte separation method when assessing immune status in human disease by flow cytometry. It offers the following advantages: (i) it does not require additional steps, (ii) it permits two-color immunofluorescence through the labeling of white blood cells after washing, (iii) it is reliable, (iv) it is reproducible, and (v) it is helpful in studies of lymphopenia since it offers the possibility of lymphocyte enrichment.

  14. Two-color SERS microscopy for protein co-localization in prostate tissue with primary antibody-protein A/G-gold nanocluster conjugates

    NASA Astrophysics Data System (ADS)

    Salehi, Mohammad; Schneider, Lilli; Ströbel, Philipp; Marx, Alexander; Packeisen, Jens; Schlücker, Sebastian

    2014-01-01

    SERS microscopy is a novel staining technique in immunohistochemistry, which is based on antibodies labeled with functionalized noble metal colloids called SERS labels or nanotags for optical detection. Conventional covalent bioconjugation of these SERS labels cannot prevent blocking of the antigen recognition sites of the antibody. We present a rational chemical design for SERS label-antibody conjugates which addresses this issue. Highly sensitive, silica-coated gold nanoparticle clusters as SERS labels are non-covalently conjugated to primary antibodies by using the chimeric protein A/G, which selectively recognizes the Fc part of antibodies and therefore prevents blocking of the antigen recognition sites. In proof-of-concept two-color imaging experiments for the co-localization of p63 and PSA on non-neoplastic prostate tissue FFPE specimens, we demonstrate the specificity and signal brightness of these rationally designed primary antibody-protein A/G-gold nanocluster conjugates.SERS microscopy is a novel staining technique in immunohistochemistry, which is based on antibodies labeled with functionalized noble metal colloids called SERS labels or nanotags for optical detection. Conventional covalent bioconjugation of these SERS labels cannot prevent blocking of the antigen recognition sites of the antibody. We present a rational chemical design for SERS label-antibody conjugates which addresses this issue. Highly sensitive, silica-coated gold nanoparticle clusters as SERS labels are non-covalently conjugated to primary antibodies by using the chimeric protein A/G, which selectively recognizes the Fc part of antibodies and therefore prevents blocking of the antigen recognition sites. In proof-of-concept two-color imaging experiments for the co-localization of p63 and PSA on non-neoplastic prostate tissue FFPE specimens, we demonstrate the specificity and signal brightness of these rationally designed primary antibody-protein A/G-gold nanocluster conjugates

  15. New Evaluated Semi-Empirical Formula Using Optical Model for 14-15 MeV ( n, t) Reaction Cross Sections

    NASA Astrophysics Data System (ADS)

    Tel, E.; Durgu, C.; Aydın, A.; Bölükdemir, M. H.; Kaplan, A.; Okuducu, Ş.

    2009-12-01

    In the next century the world will face the need for new energy sources. Nuclear fusion can be one of the most attractive sources of energy from the viewpoint of safety and minimal environmental impact. Fusion will not produce CO2 or SO2 and thus will not contribute to global warming or acid rain. Achieving acceptable performance for a fusion power system in the areas of economics, safety and environmental acceptability, is critically dependent on performance of the blanket and diverter systems which are the primary heat recovery, plasma purification, and tritium breeding systems. Tritium self-sufficiency must be maintained for a commercial power plant. The hybrid reactor is a combination of the fusion and fission processes. For self-sustaining (D-T) fusion driver tritium breeding ratio should be greater than 1.05. So working out the systematics of ( n, t) reaction cross-sections are of great importance for the definition of the excitation function character for the given reaction taking place on various nuclei at energies up to 20 MeV. In this study, we have calculated non-elastic cross-sections by using optical model for ( n, t) reactions at 14-15 MeV energy. We have investigated the excitation function character and reaction Q-values depending on the asymmetry term effect for the ( n, t) reaction cross-sections. We have obtained new coefficients for the ( n, t) reaction cross-sections. We have suggested semi-empirical formulas including optical model nonelastic effects by fitting two parameters for the ( n, t) reaction cross-sections at 14-15 MeV. We have discussed the odd-even effect and the pairing effect considering binding energy systematic of the nuclear shell model for the new experimental data and new cross-sections formulas ( n, t) reactions developed by Tel et al. We have determined a different parameter groups by the classification of nuclei into even-even, even-odd and odd-even for ( n, t) reactions cross-sections. The obtained cross-section formulas

  16. An absolute photometric system at 10 and 20 microns

    NASA Technical Reports Server (NTRS)

    Rieke, G. H.; Lebofsky, M. J.; Low, F. J.

    1985-01-01

    Two new direct calibrations at 10 and 20 microns are presented in which terrestrial flux standards are referred to infrared standard stars. These measurements give both good agreement and higher accuracy when compared with previous direct calibrations. As a result, the absolute calibrations at 10 and 20 microns have now been determined with accuracies of 3 and 8 percent, respectively. A variety of absolute calibrations based on extrapolation of stellar spectra from the visible to 10 microns are reviewed. Current atmospheric models of A-type stars underestimate their fluxes by about 10 percent at 10 microns, whereas models of solar-type stars agree well with the direct calibrations. The calibration at 20 microns can probably be determined to about 5 percent by extrapolation from the more accurate result at 10 microns. The photometric system at 10 and 20 microns is updated to reflect the new absolute calibration, to base its zero point directly on the colors of A0 stars, and to improve the accuracy in the comparison of the standard stars.

  17. Three-micron spectroscopy of highly reddened field stars

    NASA Technical Reports Server (NTRS)

    Tapia, Mauricio; Persi, P.; Roth, M.; Ferrari-Toniolo, M.

    1989-01-01

    Broad absorption features centered at 3.45 microns and at 3.0-3.0 microns towards a number of late-type supergiants in the vicinity of the galactic center were repeatedly reported. Here, 2.0 to 2.5 and 3.0 to 4.0 micron spectra are presented for field late-type highly reddened (A sub V is approximately 17-27) stars located in different regions of the galactic plane more than 20 deg away from the galactic center direction. The observations, made with the 3.6, 2.2, and 1.0 m ESO telescopes at La Silla, Chile, consists of CVF spectra with resolution lambda/delta lambda is approximately or equal to 100 and IRSPEC spectra with resolution lambda/delta lambda is approximately or equal to 700. In the direction of the most highly reddened stars, definitive detections of the 3.45 and the 3.0 to 3.1 micron absorption features are reported. The 3.45 micron feature was attributed to absorption arising in a vibrational transition resulting from the C-H stretching in organic compounds, while the 3.0 to 3.1 micron broader feature are tentatively attributed to O-H bonds. The observations strongly support that the agent producing the 3.45 micron feature, presumably organic molecules, is an important component of the diffuse interstellar medium and is not characteristic only of the galactic center environment.

  18. An absolute photometric system at 10 and 20 microns

    NASA Technical Reports Server (NTRS)

    Rieke, G. H.; Lebofsky, M. J.; Low, F. J.

    1985-01-01

    Two new direct calibrations at 10 and 20 microns are presented in which terrestrial flux standards are referred to infrared standard stars. These measurements give both good agreement and higher accuracy when compared with previous direct calibrations. As a result, the absolute calibrations at 10 and 20 microns have now been determined with accuracies of 3 and 8 percent, respectively. A variety of absolute calibrations based on extrapolation of stellar spectra from the visible to 10 microns are reviewed. Current atmospheric models of A-type stars underestimate their fluxes by about 10 percent at 10 microns, whereas models of solar-type stars agree well with the direct calibrations. The calibration at 20 microns can probably be determined to about 5 percent by extrapolation from the more accurate result at 10 microns. The photometric system at 10 and 20 microns is updated to reflect the new absolute calibration, to base its zero point directly on the colors of A0 stars, and to improve the accuracy in the comparison of the standard stars.

  19. A high resolution atlas of the galactic plane at 12 microns and 25 microns

    NASA Technical Reports Server (NTRS)

    Price, Stephan D.; Korte, Rose M.; Sample, Rebecca S.; Kennealy, John P.; Gonsalves, Robert A.

    1994-01-01

    High resolution images of the 12 micron and 25 micron IRAS survey data from each HCON crossing the Galactic Plane are being created for those regions that the original IRAS processing labeled as confused. This encompasses the area within 100 deg longitude of the Galactic Center and within 3 deg to 10 deg of the Plane. The procedures used to create the images preserve the spatial resolution inherent in the IRAS instrument. The images are separated into diffuse and point source components and candidate sources are extracted from the point source image after non-linear spatial sharpening. Fluxes are estimated by convolving the candidate sources with the point response function and cross-correlating with the original point source image. A source is considered real if it is seen on at least two HCON's with a rather generous flux match but a stringent position criterion. A number of fields spanning a range of source densities from low to high have been examined. Initial analysis indicates that the imaging and extraction works quite well up to a source density of about 100 sources per square degree or down to roughly 0.8 Janskys.

  20. Direct femtosecond laser writing system for sub-micron and micron scale patterning

    NASA Astrophysics Data System (ADS)

    Vanagas, Egidijus; Tuzhilin, Dmitry; Zinkou, Michail; Sedunov, Alexander; Vasiliev, Nikolai; Kudryashov, Igor; Kononov, Vladimir; Suruga, Shozi

    2003-11-01

    Commercial femtosecond micromachining system (FMS) has been developed that capable to process the material in sub-micron (< 200 nm) and micron scale. Core of the system are: optical unit, controller unit and software. The other parts: fs-laser system; focusing unit; stage unit can be varied (exchangeable). Two different fs-laser systems already are compatible with core of FMS: Mira/RegA (Coherent) and Hurricane (Spectra-Physics). FMS controller unit allows to control every single fs-pulse delivery on the target. Three possible types of focusing unit are available: microscope type unit, long focal distance lens unit, and axicon lens based unit. Standard stage unit options are: three-axis piezostage, and two-axis air bearing stage combined with Z-axis piezostage. Repeatability for all dimensions is within +/-5 nm. Also, step motor stages are available. The system allows 3D scan with confocal laser-microscope (resolution δr=200nm, δz=540nm) build in optical unit. Software controls all basic functions of the system performance and writing any pattern (including 3D) on or into specimen. The results obtained by direct fs-laser writing method are presented and discussed: bits in the range of 100 - 200 nm sizes, 6 TB/cm3 density optical storage matrix, waveguides fabrication inside transparent materials, high aspect ratio (1:125) patterning of dielectric materials with Gauss-Bessel beam.

  1. Cloud properties inferred from 8-12 micron data

    NASA Technical Reports Server (NTRS)

    Strabala, Kathleen I.; Ackerman, Steven A.; Menzel, W. Paul

    1994-01-01

    A trispectral combination of observations at 8-, 11-, and 12-micron bands is suggested for detecting cloud and cloud properties in the infrared. Atmospheric ice and water vapor absorption peak in opposite halves of the window region so that positive 8-minus-11-micron brightness temperature differences indicate cloud, while near-zero or negative differences indicate clear regions. The absorption coefficient for water increases more between 11 and 12 microns than between 8 and 11 microns, while for ice, the reverse is true. Cloud phases is determined by a scatter diagram of 8-minus-11-micron versus 11-minus-12-micron brightness temperature differences; ice cloud shows a slope greater than 1 and water cloud less than 1. The trispectral brightness temperature method was tested upon high-resolution interferometer data resulting in clear-cloud and cloud-phase delineation. Simulations using differing 8-micron bandwidths revealed no significant degradation of cloud property detection. Thus, the 8-micron bandwidth for future satellites can be selected based on the requirements of other applications, such as surface characterization studies. Application of the technique to current polar-orbiting High-Resolution Infrared Sounder (HIRS)-Advanced Very High Resolution Radiometer (AVHRR) datasets is constrained by the nonuniformity of the cloud scenes sensed within the large HIRS field of view. Analysis of MAS (MODIS Airborne Simulator) high-spatial resolution (500 m) data with all three 8-, 11-, and 12-micron bands revealed sharp delineation of differing cloud and background scenes, from which a simple automated threshold technique was developed. Cloud phase, clear-sky, and qualitative differences in cloud emissivity and cloud height were identified on a case study segment from 24 November 1991, consistent with the scene. More rigorous techniques would allow further cloud parameter clarification. The opportunities for global cloud delineation with the Moderate-Resolution Imaging

  2. Cloud properties inferred from 8-12 micron data

    NASA Technical Reports Server (NTRS)

    Strabala, Kathleen I.; Ackerman, Steven A.; Menzel, W. Paul

    1994-01-01

    A trispectral combination of observations at 8-, 11-, and 12-micron bands is suggested for detecting cloud and cloud properties in the infrared. Atmospheric ice and water vapor absorption peak in opposite halves of the window region so that positive 8-minus-11-micron brightness temperature differences indicate cloud, while near-zero or negative differences indicate clear regions. The absorption coefficient for water increases more between 11 and 12 microns than between 8 and 11 microns, while for ice, the reverse is true. Cloud phases is determined by a scatter diagram of 8-minus-11-micron versus 11-minus-12-micron brightness temperature differences; ice cloud shows a slope greater than 1 and water cloud less than 1. The trispectral brightness temperature method was tested upon high-resolution interferometer data resulting in clear-cloud and cloud-phase delineation. Simulations using differing 8-micron bandwidths revealed no significant degradation of cloud property detection. Thus, the 8-micron bandwidth for future satellites can be selected based on the requirements of other applications, such as surface characterization studies. Application of the technique to current polar-orbiting High-Resolution Infrared Sounder (HIRS)-Advanced Very High Resolution Radiometer (AVHRR) datasets is constrained by the nonuniformity of the cloud scenes sensed within the large HIRS field of view. Analysis of MAS (MODIS Airborne Simulator) high-spatial resolution (500 m) data with all three 8-, 11-, and 12-micron bands revealed sharp delineation of differing cloud and background scenes, from which a simple automated threshold technique was developed. Cloud phase, clear-sky, and qualitative differences in cloud emissivity and cloud height were identified on a case study segment from 24 November 1991, consistent with the scene. More rigorous techniques would allow further cloud parameter clarification. The opportunities for global cloud delineation with the Moderate-Resolution Imaging

  3. A Euploid Line of Human Embryonic Stem Cells Derived from a 43,XX,dup(9q),+12,-14,-15,-18,-21 Embryo

    PubMed Central

    Fonseca, Simone Aparecida Siqueira; Costas, Roberta Montero; Morato-Marques, Mariana; Costa, Silvia; Alegretti, Jose Roberto; Rosenberg, Carla; da Motta, Eduardo Leme Alves; Serafini, Paulo C.; Pereira, Lygia V.

    2015-01-01

    Aneuploid embryos diagnosed by FISH-based preimplantation genetic screening (PGS) have been shown to yield euploid lines of human embryonic stem cells (hESCs) with a relatively high frequency. Given that the diagnostic procedure is usually based on the analysis of 1–2 blastomeres of 5 to 10-cell cleavage-stage embryos, mosaicism has been a likely explanation for the phenomena. However, FISH-based PGS can have a significant rate of misdiagnosis, and therefore some of those lines may have been derived from euploid embryos misdiagnosed as aneuploid. More recently, coupling of trophectoderm (TE) biopsy at the blastocyst stage and array-CGH lead to a more informative form of PGS. Here we describe the establishment of a new line of hESCs from an embryo with a 43,XX,dup(9q),+12,-14,-15,-18,-21 chromosomal content based on array-CGH of TE biopsy. We show that, despite the complex chromosomal abnormality, the corresponding hESC line BR-6 is euploid (46,XX). Single nucleotide polymorphism analysis showed that the embryo´s missing chromosomes were not duplicated in BR-6, suggesting the existence of extensive mosaicism in the TE lineage. PMID:26540511

  4. Flood of June 14-15, 1990, in Belmont, Jefferson, and Harrison counties, Ohio, with emphasis on Pipe and Wegee Creek basins near Shadyside

    USGS Publications Warehouse

    Shindel, H.L.

    1991-01-01

    A series of violent thunderstorms caused severe floods and consequent damage in the central part of Ohio during June 14-15, 1990. The eastern part of the State, particularly Belmont, Harrison, and Jefferson Counties, sustained the most damage. In the Pipe and Wegee Creek basins near Shadyside, Belmont County, at least 24 people died and property damage exceeded $10 million. An indirect measurement of discharge on Pipe Creek made near the mouth, indicates a peak discharge of 15,000 ft? /s (cubic feet per second) for the drainage area of 11.3 mi? (square miles) and a unit discharge of 1,330 (ft? /s)/mi? (cubic feet per second per square mile). The recurrence interval for this peak discharge is greater than 100 years. An indirect measurement of discharge of Wegee Creek, made 3 miles upstream from the mouth, indicates a peak discharge of 2,200 (ft? /s) /mi? . The recurrence interval for this peak discharge also is greater than 100 years. Longitudal water-surface profiles showed depths ranging from 7 ft. to 22 ft. The severity of flooding was highly variable. For example, the recurrence interval of the peak discharge at one U.S. Geological Survey streamflow-gaging station was only 2 years, whereas the recurrence interval for the peak discharge was greater than 100 years at another gaging station about 22 miles away.

  5. Antibacterial activity of 14, 15-dihydroajugapitin and 8-o-acetylharpagide isolated from Ajuga bracteosa Wall ex. Benth against human pathogenic bacteria.

    PubMed

    Ganaie, Hilal A; Ali, Md Niamat; Ganai, Bashir A; Meraj, Maryum; Ahmad, Mudasar

    2017-02-01

    Ajuga bracteosa Wall ex. Benth. (Lamiaceae) commonly known as Bungle Weed has been in use since ancient times and is mentioned Ayurvedic literature. The upper ground parts of the plant are used for treatment of various diseases. The weed is credited with astringent, febrifugal, stimulant, aperient, tonic, diuretic and depurative properties and is used for the treatment of gout and rheumatism, palsy and amenorrhoea. Two compounds 1) 14, 15-dihydroajugapitin and 2) 8-o-acetylharpagide were isolated from the aerial parts of the plant and tested for antibacterial activity against various pathogenic bacteria by agar well diffusion method. Compound 1 and 2 showed maximum antibacterial activity against Escherichia coli with zone of inhibitions of 25. 0 ± 1.4 mm and 22.6 ± 0.9 mm respectively. The MIC value of compound 1 and 2 ranged between 500 and 1000 μg/ml. It could be concluded that both compounds isolated from the aerial parts of Ajuga bracteosa possess antibacterial activity against pathogens. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. The Ubiquitin Receptors DA1, DAR1, and DAR2 Redundantly Regulate Endoreduplication by Modulating the Stability of TCP14/15 in Arabidopsis

    PubMed Central

    Peng, Yuancheng; Chen, Liangliang; Lu, Yaru; Wu, Yingbao; Dumenil, Jack; Zhu, Zhengge; Bevan, Michael W.; Li, Yunhai

    2015-01-01

    Organ growth involves the coordination of cell proliferation and cell growth with differentiation. Endoreduplication is correlated with the onset of cell differentiation and with cell and organ size, but little is known about the molecular mechanisms linking cell and organ growth with endoreduplication. We have previously demonstrated that the ubiquitin receptor DA1 influences organ growth by restricting cell proliferation. Here, we show that DA1 and its close family members DAR1 and DAR2 are redundantly required for endoreduplication during leaf development. DA1, DAR1, and DAR2 physically interact with the transcription factors TCP14 and TCP15, which repress endoreduplication by directly regulating the expression of cell-cycle genes. We also show that DA1, DAR1, and DAR2 modulate the stability of TCP14 and TCP15 proteins in Arabidopsis thaliana. Genetic analyses demonstrate that DA1, DAR1, and DAR2 function in a common pathway with TCP14/15 to regulate endoreduplication. Thus, our findings define an important genetic and molecular mechanism involving the ubiquitin receptors DA1, DAR1, and DAR2 and the transcription factors TCP14 and TCP15 that links endoreduplication with cell and organ growth. PMID:25757472

  7. The ubiquitin receptors DA1, DAR1, and DAR2 redundantly regulate endoreduplication by modulating the stability of TCP14/15 in Arabidopsis.

    PubMed

    Peng, Yuancheng; Chen, Liangliang; Lu, Yaru; Wu, Yingbao; Dumenil, Jack; Zhu, Zhengge; Bevan, Michael W; Li, Yunhai

    2015-03-01

    Organ growth involves the coordination of cell proliferation and cell growth with differentiation. Endoreduplication is correlated with the onset of cell differentiation and with cell and organ size, but little is known about the molecular mechanisms linking cell and organ growth with endoreduplication. We have previously demonstrated that the ubiquitin receptor DA1 influences organ growth by restricting cell proliferation. Here, we show that DA1 and its close family members DAR1 and DAR2 are redundantly required for endoreduplication during leaf development. DA1, DAR1, and DAR2 physically interact with the transcription factors TCP14 and TCP15, which repress endoreduplication by directly regulating the expression of cell-cycle genes. We also show that DA1, DAR1, and DAR2 modulate the stability of TCP14 and TCP15 proteins in Arabidopsis thaliana. Genetic analyses demonstrate that DA1, DAR1, and DAR2 function in a common pathway with TCP14/15 to regulate endoreduplication. Thus, our findings define an important genetic and molecular mechanism involving the ubiquitin receptors DA1, DAR1, and DAR2 and the transcription factors TCP14 and TCP15 that links endoreduplication with cell and organ growth. © 2015 American Society of Plant Biologists. All rights reserved.

  8. Investigation of 14-15 MeV ( n, t) Reaction Cross-sections by Using New Evaluated Empirical and Semi-empirical Systematic Formulas

    NASA Astrophysics Data System (ADS)

    Tel, E.; Aydın, A.; Kaplan, A.; Şarer, B.

    2008-09-01

    In the hybrid reactor, tritium self-sufficiency must be maintained for a commercial power plant. For self-sustaining (D-T) fusion driver tritium breeding ratio should be greater than 1.05. Working out the systematics of ( n, t) reaction cross-sections are of great importance for the definition of the excitation function character for the given reaction taking place on various nuclei at energies up to 20 MeV. In this study we have investigated asymmetry term effect for the ( n, t) reaction cross-sections at 14-15 neutron incident energy. It has been discussed the odd-even effect and the pairing effect considering binding energy systematic of the nuclear shell model for the new experimental data and new cross-sections formulas ( n, t) reactions developed by Tel et al. We have determined a different parameter groups by the classification of nuclei into even-even, even-odd and odd-even for ( n, t) reactions cross-sections. The obtained empirical and semi-empirical formulas by fitting two parameter for ( n, t) reactions were given. All calculated results have been compared with the experimental data and the other semi-empirical formulas.

  9. Two color machining of composites

    SciTech Connect

    Maher, W.

    1996-12-31

    Quality cutting and drilling of 9 composite materials is possible using diode-pumped neodymium lasers. Machining at 1.06 {mu}m is improved by optimizing the pulse format of the diode-pumped neodymium lasers of the Precision Laser Machining (PLM) Consortium. Further improvements are obtained with the PLM lasers operating at 0.53 {mu}m.

  10. On the possible bipolar nature of 21 micron IRAS sources

    NASA Technical Reports Server (NTRS)

    Hrivnak, Bruce J.; Kwok, S.

    1991-01-01

    The discovery of another IRAS source (22574 + 6609) showing the unidentified 21-micron emission feature is reported. Its overall energy distribution is similar to the well-known edge-on bipolar nebulae AFGL 2688 and AFGL 618. Ground-based optical and infrared observations of this object and two other 21-micron sources show that while all three have very similar infrared properties, they differ greatly in the visual region. All three of these 21-micron sources are intrinsically similar bipolar nebulae, viewed at different orientations.

  11. Use of a Novel Two Color PALM Method to Examine Structural Properties of Drp1 Helical Rings during Mammalian Mitochondrial Fission In Situ

    NASA Astrophysics Data System (ADS)

    Rosenbloom, Alyssa Blair

    In this thesis, we accomplish two goals: 1) we develop a novel two color photoactivatable light microscopy (PALM) method for imaging in mammalian cells and 2) we explore our original biological question and discern the structural properties of the Drp1 helical ring during fission. We established that mitochondrial membranes can be distinguished with the available photoactivatable fluorescent protein mEos2. However, we were not able to use any of the published photoactivatable and photoswitchable green fluorescent proteins, predominantly because of an inability to identify individual fluorescent events due to rapidity of the photoswitiching. Based on published crystal structures, we created novel Dronpa variants with increasing steric hindrance around the chromophore, likely partially inhibiting the isomerization. We replaced Val157 with isoleucine, leucine, or phenyalanine. DronpaV157F showed no fluorescence and was discarded. DronpaV157I and DronpaV157L showed photoswitchable green fluorescence, with individual fluorescent events that were more easily discerned. DronpaV157L in particular had bright fluorescent events that were well separated when imaged in mammalian cells at 20 Hz. We named this new variant rsKame. Using PALM we successfully imaged rsKame expressed and localized to the mammalian mitochondrial inner membrane. With the novel photoswitchable fluorescent protein, rsKame, available, we returned to the development of a novel two color PALM method. We chose PAmCherry1 as the partner for rsKame since PAmCherry1 has distinct and well separated excitation/emission spectra from rsKame and is not activated by low 405 nm laser power density. We first imaged rsKame with 405 nm activation at (0.61 mW/mm2) and 488 nm activation/excitation (5.87 W/mm 2) to completion. We then imaged PAmCherry1 with increasing 405 nm activation (0.6-6.0 W/mm2) and 561 nm excitation (22 W/mm 2). With the novel PALM imaging method, we labeled the inner and outer mitochondrial

  12. Improved Fabrication of Lithium Films Having Micron Features

    NASA Technical Reports Server (NTRS)

    Whitacre, Jay

    2006-01-01

    An improved method has been devised for fabricating micron-dimension Li features. This approach is intended for application in the fabrication of lithium-based microelectrochemical devices -- particularly solid-state thin-film lithium microbatteries.

  13. Release of Micronized Copper Particles from Pressure Treated Wood Products.

    EPA Science Inventory

    Micronized copper pressure treated lumber (PTL) has recently been introduced to the consumer market as a replacement for ionized copper PTL. The presence of particulate rather than aqueous copper raises concerns about possible human or environmental exposure. Two common pathways ...

  14. Release of Micronized Copper Particles from Pressure Treated Wood Products.

    EPA Science Inventory

    Micronized copper pressure treated lumber (PTL) has recently been introduced to the consumer market as a replacement for ionized copper PTL. The presence of particulate rather than aqueous copper raises concerns about possible human or environmental exposure. Two common pathways ...

  15. Airborne spectrophotometry of Comet Halley from 5 to 9 microns

    NASA Technical Reports Server (NTRS)

    Campins, H.; Bregman, J. D.; Witteborn, F. C.; Wooden, D. H.; Rank, D. M.; Cohen, M.; Allamandola, Louis J.; Tielens, Alexander G. G. M.

    1986-01-01

    Spectrophotometry from 5 to 9 microns (resolution = 0.02) of comet Halley was obtained from the Kuiper Airborne Observatory on 1985 Dec. 12.1 and 1986 April 8.6 and 10.5 UT. Two spectral features are apparent in all the observations, one from 5.24 to 5.6 microns, and the silicate emission feature which has an onset between 7 and 8 microns. There is no evidence for the 7.5 microns feature observed by the Vega 1 spacecraft; the large difference between the areal coverage viewed from the spacecraft and the airplane may explain the discrepancy. Color temperatures significantly higher than a blackbody indicate that small particles are abundant in the coma. Significant spatial and temporal variations in the spectrum show trends similar to those observed from the ground.

  16. Injection Seeded/Phase-Conjugated 2-micron Laser System

    NASA Technical Reports Server (NTRS)

    Bai, Yingxin; Yu, Jirong; Petros,M.; Petzar, Paul; Trieu, Bo; Lee, Hyung; Singh, U.; Leyva, V.; Shkunov, V.; Rockwell, D.; hide

    2007-01-01

    For the first time, beam quality improvement of 2 micron laser using a fiber based phase conjugation mirror has been demonstrated. Single frequency operation is necessary to lower threshold. The reflectivity of PCM is approx. 50%.

  17. Apparatus for handling micron size range particulate material

    NASA Technical Reports Server (NTRS)

    Friichtenicht, J. F.; Roy, N. L. (Inventor)

    1968-01-01

    An apparatus for handling, transporting, or size classifying comminuted material was described in detail. Electrostatic acceleration techniques for classifying particles as to size in the particle range from 0.1 to about 100 microns diameter were employed.

  18. Characterization of Micron-Scale Nanotublar Super Dielectric Materials

    DTIC Science & Technology

    2015-09-01

    MICRON-SCALE NANOTUBULAR SUPER DIELECTRIC MATERIALS by Jonathan Wayne Gandy September 2015 Thesis Advisor: Jonathan Phillips Co-Advisor...AND DATES COVERED Master’s Thesis 4. TITLE AND SUBTITLE CHARACTERIZATION OF MICRON-SCALE NANOTUBULAR SUPER DIELECTRIC MATERIALS 5. FUNDING NUMBERS...study focused on dielectric materials based on a novel hypothesis: that porous electrically insulating solids in which the pores are filled with liquids

  19. High-resolution maps of Jupiter at five microns.

    NASA Technical Reports Server (NTRS)

    Keay, C. S. L.; Low, F. J.; Rieke, G. H.; Minton, R. B.

    1973-01-01

    The distribution of 5-micron radiation, emitted from a large number of discrete sources from Jupiter, was observed during the 1972 apparition. These sources are less bright than those observed by Westphal (1969). At least 50 discrete sources having brightness temperatures exceeding 227 K were revealed which were mainly located within three narrow-latitude bands. Strong correlation exists between the 5-micron brightness temperatures of Jovian features and their colors as recorded photographically.

  20. Micron Accuracy Deployment Experiment (MADE), phase A. Volume 1

    NASA Technical Reports Server (NTRS)

    Peterson, Lee D.; Lake, Mark S.

    1995-01-01

    This report documents a Phase A In-STEP flight experiment development effort. The objective of the experiment is to deploy a portion of a segmented reflector on the Shuttle and study its micron-level mechanics. Ground test data are presented which projects that the on-orbit precision of the test article should be approximately 5 microns. Extensive hardware configuration development information is also provided.

  1. Laser materials for the 0.67-microns to 2.5-microns range

    NASA Technical Reports Server (NTRS)

    Toda, Minoru; Zamerowski, Thomas J.; Ladany, Ivan; Martinelli, Ramon U.

    1987-01-01

    Basic requirements for obtaining injection laser action in III-V semiconductors are discussed briefly. A detailed review is presented of materials suitable for lasers emitting at 0.67, 1.44, 1.93, and 2.5 microns. A general approach to the problem is presented, based on curves of materials properties published by Sasaki et al. It is also shown that these curves, although useful, may need correction in certain ranges. It is deduced that certain materials combinations, either proposed in the literature or actually tried, are not appropriate for double heterostructure lasers, because the refractive index of the cladding material is higher than the index of the active material, thus resulting in no waveguiding, and high threshold currents. Recommendations are made about the most promising approach to the achievement of laser action in the four wavelengths mentioned above.

  2. Simultaneous scoring of 10 chromosomes (9,13,14,15,16,18,21,22,X, and Y) in interphase nuclei by using spectral imaging

    NASA Astrophysics Data System (ADS)

    Fung, Jingly; Weier, Heinz-Ulli G.; Goldberg, James D.; Pedersen, Roger A.

    1999-06-01

    Numerical aberrations involving parts of or entire chromosomes have detrimental effects on mammalian embryonic, and perinatal development. Only few fetuses with chromosomal imbalances survive to term, and their abnormalities lead to stillbirth or cause severely altered phenotypes in the offspring (such as trisomies involving chromosomes 13, 18, 21, and anomalies of X, and Y). Because aneuploidy of any of the 24 chromosomes will have significant consequences, an optimized preimplantation and prenatal genetic diagnosis (PGD) test will score all the chromosomes. Since most cells to be analyzed will be in interphase rather than metaphase, we developed a rapid procedure for the analysis of interphase cells such as lymphocytes, amniocytes, or early embryonic cells (blastomeres). Our approach was based on in situ hybridization of chromosome-specific non-isotopically labeled DNA probes and Spectral Imaging. The Spectral Imaging system uses an interferometer instead of standard emission filters in a fluorescence microscope to record high resolution spectra from fluorescently stained specimens. This bio-imaging system combines the techniques of fluorescence optical microscopy, charged coupled device imaging, Fourier spectroscopy, light microscopy, and powerful analysis software. The probe set used here allowed simultaneous detection of 10 chromosomes (9, 13, 14, 15, 16, 18, 21, 22, X, Y) in interphase nuclei. Probes were obtained commercially or prepared in-house. Following 16 - 40 h hybridization to interphase cells and removal of unbound probes, image spectra (range 450 - 850 nm, resolution 10 nm) were recorded and analyzed using an SD200 Spectral Imaging system (ASI, Carlsbad, CA). Initially some amniocytes were unscoreable due to their thickness, and fixation protocols had to be modified to achieve satisfactory results. In summary, this study shows the simultaneous detection of at least 10 different chromosomes in interphase cells using a novel approach for multi

  3. Hearing and loud music exposure in a group of adolescents at the ages of 14-15 and retested at 17-18.

    PubMed

    Biassoni, Ester C; Serra, Mario R; Hinalaf, María; Abraham, Mónica; Pavlik, Marta; Villalobo, Jorge Pérez; Curet, Carlos; Joekes, Silvia; Yacci, María R; Righetti, Andrea

    2014-01-01

    Young people expose themselves to potentially damaging loud sounds while leisure activities and noise induced hearing loss is diagnosed in increasing number of adolescents. Hearing and music exposure in a group of adolescents of a technical high school was assessed at the ages of: 14-15 (test) and 17-18 (retest). The aims of the current study were: (1) To compare the auditory function between test and retest; (2) to compare the musical exposure levels during recreational activities in test and retest; (3) to compare the auditory function with the musical exposure along time in a subgroup of adolescents. The participants in the test were 172 male; in the retest, this number was reduced to 59. At the test and retest the conventional and extended high frequency audiometry, transient evoked otoacoustic emissions (TEOAEs) and recreational habits questionnaire were performed. In the test, hearing threshold levels (HTLs) were classified as: Normal (Group 1), slightly shifted (Group 2), and significantly shifted (Group 3); the Musical General Exposure (MGE), categorized in: Low, moderate, high, and very high exposure. The results revealed a significant difference (P < 0.0001) between test and retest in the HTL and global amplitude of TEOAEs in Group 1, showing an increase of the HTL and a decrease TEOAEs amplitude. A subgroup of adolescents, with normal hearing and low exposure to music in the test, showed an increase of the HTL according with the categories of MGE in the retest. To implement educational programs for assessing hearing function, ear vulnerability and to promote hearing health, would be advisable.

  4. Micronized Organic Magnesium Salts Enhance Opioid Analgesia in Rats

    PubMed Central

    Bujalska-Zadrożny, Magdalena; Kulik, Kamila; Ordak, Michał; Sasinowska-Motyl, Małgorzata; Gąsińska, Emilia; de Corde, Anna; Kowalczyk, Agnieszka; Sacharczuk, Mariusz; Naruszewicz, Marek

    2016-01-01

    Purpose As previously reported, magnesium sulphate administered parenterally significantly increased an opioid antinociception in different kinds of pain. Since the typical form of magnesium salts are poorly and slowly absorbed from the gastrointestinal tract we examined whether their micronized form could increase opioids induced antinociception. Methods In behavioural studies on rats morphine, tramadol and oxycodone together with magnesium (lactate dihydrate, hydroaspartate, chloride) in micronized (particles of size D90 < 50 μm) and conventional forms were used. Changes in pain thresholds were determined using mechanical stimuli. The intestinal absorption of two forms of magnesium lactate dihydrate (at the doses of 7.5 or 15 mg ions) in the porcine gut sac model were also compared. Results Micronized form of magnesium lactate dihydrate or hydroaspartate but not chloride (15 mg of magnesium ions kg-1) enhanced the analgesic activity of orally administered opioids, significantly faster and more effective in comparison to the conventional form of magnesium salts (about 40% for oxycodone administered together with a micronized form of magnesium hydroaspartate). Moreover, in vitro studies of transport across porcine intestines of magnesium ions showed that magnesium salts administered in micronized form were absorbed from the intestines to a greater extent than the normal form of magnesium salts. Conclusions The co-administration of micronized magnesium organic salts with opioids increased their synergetic analgesic effect. This may suggest an innovative approach to the treatment of pain in clinical practice. PMID:27792736

  5. The NH3 spectrum in Saturn's 5 micron window

    NASA Technical Reports Server (NTRS)

    Bjoraker, G. L.; Fink, U.; Larson, H. P.; Johnson, J. R.

    1983-01-01

    Spectra of Saturn's 5-micron window were obtained at the Infrared Telescope Facility on Mauna Kea, Hawaii. The spectra have a resolution of 1.2/cm, and some exhibit extremely low amounts of approximately 300-micron ppt telluric H2O. The Saturn spectra show absorptions by the 2nu2 band of NH3. Long-path laboratory comparison spectra of NH3 were acquired and show considerable deviations in intensity from theoretical predictions. The calibration of Saturn's observed NH3 features with the laboratory data gives 2.0 + or - 0.5 m-amagat of NH3 using the 2nu2 Q-branch at 5.32 microns. The R(1) and R(2) lines yield an abundance about 3 times greater. Absorptions outside the range of the Q-branch can be accounted for by solid NH3 of 10-20 microns equivalent path length. The origin of Saturn's 5-micron flux is mostly thermal with some admixture of solar reflected radiation. A depletion of Saturn's NH3 abundance below the solar value is indicated, but confirmation of this conclusion will require a better understanding of the atmospheric penetration depth at 5 microns and more rigorous modeling of the spectral line formation.

  6. Phosphine absorption in the 5-micron window of Jupiter

    NASA Technical Reports Server (NTRS)

    Beer, R.; Taylor, F. W.

    1979-01-01

    Since the original suggestion by Gillett et al. (1969) it has generally been assumed that the region of partial transparency near 5 micron in Jupiter's atmosphere (the 5-micron window) is bounded by the nu sub 4 NH3 at 6.1 micron and the nu sub 3 CH4 band at 3.3 micron. New measurements of Jupiter and of laboratory phosphine (PH3) samples show that PH3 is a significant contributor to the continuum opacity in the window and in fact defines its short-wavelength limit. This has important implications for the use of 5-micron observations as a means to probe the deep atmospheric structure of Jupiter. The abundance of PH3 which results from a comparison of Jovian and laboratory spectra is about 3 to 5 cm-am. This is five to eight times less than that found by Larson et al. (1977) in the same spectral region, but is in good agreement with the result of Tokunaga et al. (1979) from 10-micron observations.

  7. Phosphine absorption in the 5-micron window of Jupiter

    NASA Technical Reports Server (NTRS)

    Beer, R.; Taylor, F. W.

    1979-01-01

    Since the original suggestion by Gillett et al. (1969) it has generally been assumed that the region of partial transparency near 5 micron in Jupiter's atmosphere (the 5-micron window) is bounded by the nu sub 4 NH3 at 6.1 micron and the nu sub 3 CH4 band at 3.3 micron. New measurements of Jupiter and of laboratory phosphine (PH3) samples show that PH3 is a significant contributor to the continuum opacity in the window and in fact defines its short-wavelength limit. This has important implications for the use of 5-micron observations as a means to probe the deep atmospheric structure of Jupiter. The abundance of PH3 which results from a comparison of Jovian and laboratory spectra is about 3 to 5 cm-am. This is five to eight times less than that found by Larson et al. (1977) in the same spectral region, but is in good agreement with the result of Tokunaga et al. (1979) from 10-micron observations.

  8. Design and initial operation of a two-color soft x-ray camera system on the Compact Toroidal Hybrid experiment

    SciTech Connect

    Herfindal, J. L. Dawson, J. D.; Ennis, D. A.; Hartwell, G. J.; Loch, S. D.; Maurer, D. A.

    2014-11-15

    A multi-camera soft x-ray diagnostic has been developed to measure the equilibrium electron temperature profile and temperature fluctuations due to magnetohydrodynamic activity on the Compact Toroidal Hybrid experiment. The diagnostic consists of three separate cameras each employing two 20-channel diode arrays that view the same plasma region through different beryllium filter thicknesses of 1.8 μm and 3.0 μm allowing electron temperature measurements between 50 eV and 200 eV. The Compact Toroidal Hybrid is a five-field period current-carrying stellarator, in which the presence of plasma current strongly modifies the rotational transform and degree of asymmetry of the equilibrium. Details of the soft x-ray emission, effects of plasma asymmetry, and impurity line radiation on the design and measurement of the two-color diagnostic are discussed. Preliminary estimates of the temperature perturbation due to sawtooth oscillations observed in these hybrid discharges are given.

  9. The mechanics of unrest at Long Valley caldera, California: 1. Modeling the geometry of the source using GPS, leveling and two-color EDM data

    USGS Publications Warehouse

    Battaglia, Maurizio; Segall, P.; Murray, J.; Cervelli, Peter; Langbein, J.

    2003-01-01

    We surveyed 44 existing leveling monuments in Long Valley caldera in July 1999, using dual frequency global positioning system (GPS) receivers. We have been able to tie GPS and leveling to a common reference frame in the Long Valley area and computed the vertical deformation by differencing GPS-based and leveled orthometric heights. The resurgent dome uplifted 74??7 cm from 1975 to 1999. To define the inflation source, we invert two-color EDM and uplift data from the 1985-1999 unrest period using spherical or ellipsoidal sources. We find that the ellipsoidal source satisfies both the vertical and horizontal deformation data, whereas the spherical point source cannot. According to our analysis of the 1985-1999 data, the main source of deformation is a prolate ellipsoid located beneath the resurgent dome at a depth of 5.9 km (95% bounds of 4.9-7.5 km). This body is vertically elongated, has an aspect ratio of 0.475 (95% bounds are 0.25-0.65) and a volume change of 0.086 km3 (95% bounds are 0.06-0.13 km3). Failure to account for the ellipsoidal nature of the source biases the estimated source depth by 2.1 km (35%), and the source volume by 0.038 km3 (44%). ?? 2003 Elsevier B.V. All rights reserved.

  10. Development of a two-color laser fluorescence detector. On-line detection of internal standards and unknowns by capillary electrophoresis within the same sample.

    PubMed

    Morgan, Nicole Y; Wellner, Edward; Talbot, Thomas; Smith, Paul D; Phillips, Terry M

    2006-02-10

    A two-laser, two-color detector has been developed for the simultaneous detection of naturally occurring and recombinant (internal standards) cytokines within the same biological sample. The internal standards were labeled with Bimane and detected with a 408 nm laser while the natural cytokines were labeled with AlexaFluor633 and detected with a 633 nm laser. The two resulting electropherograms were plotted as overlaid traces and quantification of the natural materials determined by comparison with the standards. Using this system, recovery of all four cytokine standards was greater than 94% in both saline and cytokine-depleted plasma. These recoveries could be achieved with intra- and inter-assay coefficients of variance (c.v.) of less than 4.5 and 5.6, respectively. Application of this system to the examination of clinical samples demonstrated that measurement of the four pro-inflammatory cytokines could distinguish between normals, sub-clinical and clinical inflammation. An advantage of this approach is that direct calculation of unknowns by comparison to identical internal standards can shorten analytical time by eliminating the need for additional standard or calibration runs.

  11. Numerical Simulation of Spatial and Spectral Crosstalk in Two-Color MWIR/LWIR HgCdTe Infrared Detector Arrays

    NASA Astrophysics Data System (ADS)

    Pinkie, Benjamin; Bellotti, Enrico

    2013-11-01

    The sequential two-color Hg1- x Cd x Te architecture has emerged as a key technology in the development of third-generation infrared detectors. Due to the expense required to manufacture these devices, it is imperative to create numerical models which can predict the electrical and optical behavior of the technology as well as evaluate design concepts prior to exhaustive development. We have developed a three-dimensional simulation model which fully accounts for the optical phenomena that become increasingly important in small pixels and uses a drift-diffusion approach to determine the electrical behavior of the device. In particular, we employ a finite-difference time- domain method to solve Maxwell's equations and a finite-element method to evaluate the solutions of the coupled Poisson and carrier continuity equations. We apply our simulation model to simulate the dynamic resistance and current density versus voltage characteristics of this detector architecture. The quantum efficiency is then determined for both spectral bands while observing the effects of variable pixel pitch and detector geometry. Finally, we use a spatially finite Gaussian beam to analyze the crosstalk and perform a simulated spot scan.

  12. An approach for plasma cell myeloma diagnosis by two-color flow cytometry based on kappa/lambda ratios of CD38-gated plasma cells.

    PubMed

    Nakayama, S; Yokote, T; Hirata, Y; Iwaki, K; Akioka, T; Miyoshi, T; Takayama, A; Nishiwaki, U; Masuda, Y; Ikemoto, T; Tanaka, H; Nishimura, Y; Tsuji, M; Hanafusa, T

    2013-01-01

    Criteria from the World Health Organization (WHO) are commonly used to diagnose plasma cell myeloma (PCM), but they are complex and require several laboratory parameters. To differentiate reactive plasmacytosis from clonal plasma cell neoplasms, such as PCM, it is important to accurately determine the expression of the cytoplasmic immunoglobulin (cIg) light chain (LC). Through retrospective analyses, we selected the patients with PCM, and analyzed records of 52 PCM patients, who underwent bone biopsies, and final diagnosis of PCM was established according to WHO criteria, and 22 controls. In the present study, all samples were analyzed by flow cytometry (FC) in the side scatter vs CD38 histogram mode, and the CD38-gated plasma cell population was identified. The positive cell ratios of kappa and lambda to plasma cell populations were analyzed. PCM cells were distinguished from normal plasma cells by a cut-off level between 0.80 and 3.3, a sensitivity of 90.3 percent, and a specificity of 81.1 percent. Two-color FC analysis is simple to perform, inexpensive, and clinically relevant data are obtained soon after completion of the FC measurements. It could be one of the helpful tools in the diagnosis of PCM. The correct diagnosis of PCM can be achieved more simply, efficiently, and rapidly by combining this method.

  13. Optimal generation of spatially coherent soft X-ray isolated attosecond pulses in a gas-filled waveguide using two-color synthesized laser pulses

    PubMed Central

    Jin, Cheng; Hong, Kyung-Han; Lin, C. D.

    2016-01-01

    We numerically demonstrate the generation of intense, low-divergence soft X-ray isolated attosecond pulses in a gas-filled hollow waveguide using synthesized few-cycle two-color laser waveforms. The waveform is a superposition of a fundamental and its second harmonic optimized such that highest harmonic yields are emitted from each atom. We then optimize the gas pressure and the length and radius of the waveguide such that bright coherent high-order harmonics with angular divergence smaller than 1 mrad are generated, for photon energy from the extreme ultraviolet to soft X-rays. By selecting a proper spectral range enhanced isolated attosecond pulses are generated. We study how dynamic phase matching caused by the interplay among waveguide mode, neutral atomic dispersion, and plasma effect is achieved at the optimal macroscopic conditions, by performing time-frequency analysis and by analyzing the evolution of the driving laser’s electric field during the propagation. Our results, when combined with the on-going push of high-repetition-rate lasers (sub- to few MHz’s) may eventually lead to the generation of high-flux, low-divergence soft X-ray tabletop isolated attosecond pulses for applications. PMID:27929036

  14. Laser-induced selective area tuning of GaAs/AlGaAs quantum well microstructures for two-color IR detector operation

    NASA Astrophysics Data System (ADS)

    Dubowski, J. J.; Song, C. Y.; Lefebvre, J.; Wasilewski, Z.; Aers, G.; Liu, H. C.

    2004-05-01

    Selective area laser annealing of GaAs/AlxGa1-xAs quantum well infrared photodetector (QWIP) material has been investigated as a possible route towards the fabrication of two-color low-cost focal plane array devices. Tuning of the wavelength response of the material has been achieved as a consequence of the quantum well intermixing (QWI) effect. A 90 s irradiation with a continuous wave Nd:yttrium-aluminum-garnet laser, at the peak temperature of 850 °C, resulted in the 40 nm blueshift of the QW photoluminescence peak from 832 to 792 nm. This corresponded to the 0.7 μm redshift of the wavelength response of the investigated QWIP microstructure in the 8 μm optical absorption region. The amplitude of this shift is consistent with the literature data obtained for similar material processed directly by rapid thermal annealing (RTA) or by a two-step process involving particle implantation and RTA. We have examined the laser-QWI approach for direct writing of arrays of a two-band gap material. The preliminary results indicate the feasibility of this approach for fabricating linear arrays with a period of 0.8 mm. .

  15. Axonal Membrane Proteins Are Transported in Distinct Carriers: A Two-Color Video Microscopy Study in Cultured Hippocampal NeuronsV⃞

    PubMed Central

    Kaether, Christoph; Skehel, Paul; Dotti, Carlos G.

    2000-01-01

    Neurons transport newly synthesized membrane proteins along axons by microtubule-mediated fast axonal transport. Membrane proteins destined for different axonal subdomains are thought to be transported in different transport carriers. To analyze this differential transport in living neurons, we tagged the amyloid precursor protein (APP) and synaptophysin (p38) with green fluorescent protein (GFP) variants. The resulting fusion proteins, APP-yellow fluorescent protein (YFP), p38-enhanced GFP, and p38-enhanced cyan fluorescent protein, were expressed in hippocampal neurons, and the cells were imaged by video microscopy. APP-YFP was transported in elongated tubules that moved extremely fast (on average 4.5 μm/s) and over long distances. In contrast, p38-enhanced GFP-transporting structures were more vesicular and moved four times slower (0.9 μm/s) and over shorter distances only. Two-color video microscopy showed that the two proteins were sorted to different carriers that moved with different characteristics along axons of doubly transfected neurons. Antisense treatment using oligonucleotides against the kinesin heavy chain slowed down the long, continuous movement of APP-YFP tubules and increased frequency of directional changes. These results demonstrate for the first time directly the sorting and transport of two axonal membrane proteins into different carriers. Moreover, the extremely fast-moving tubules represent a previously unidentified type of axonal carrier. PMID:10749925

  16. Optimal generation of spatially coherent soft X-ray isolated attosecond pulses in a gas-filled waveguide using two-color synthesized laser pulses.

    PubMed

    Jin, Cheng; Hong, Kyung-Han; Lin, C D

    2016-12-08

    We numerically demonstrate the generation of intense, low-divergence soft X-ray isolated attosecond pulses in a gas-filled hollow waveguide using synthesized few-cycle two-color laser waveforms. The waveform is a superposition of a fundamental and its second harmonic optimized such that highest harmonic yields are emitted from each atom. We then optimize the gas pressure and the length and radius of the waveguide such that bright coherent high-order harmonics with angular divergence smaller than 1 mrad are generated, for photon energy from the extreme ultraviolet to soft X-rays. By selecting a proper spectral range enhanced isolated attosecond pulses are generated. We study how dynamic phase matching caused by the interplay among waveguide mode, neutral atomic dispersion, and plasma effect is achieved at the optimal macroscopic conditions, by performing time-frequency analysis and by analyzing the evolution of the driving laser's electric field during the propagation. Our results, when combined with the on-going push of high-repetition-rate lasers (sub- to few MHz's) may eventually lead to the generation of high-flux, low-divergence soft X-ray tabletop isolated attosecond pulses for applications.

  17. Optimal generation of spatially coherent soft X-ray isolated attosecond pulses in a gas-filled waveguide using two-color synthesized laser pulses

    NASA Astrophysics Data System (ADS)

    Jin, Cheng; Hong, Kyung-Han; Lin, C. D.

    2016-12-01

    We numerically demonstrate the generation of intense, low-divergence soft X-ray isolated attosecond pulses in a gas-filled hollow waveguide using synthesized few-cycle two-color laser waveforms. The waveform is a superposition of a fundamental and its second harmonic optimized such that highest harmonic yields are emitted from each atom. We then optimize the gas pressure and the length and radius of the waveguide such that bright coherent high-order harmonics with angular divergence smaller than 1 mrad are generated, for photon energy from the extreme ultraviolet to soft X-rays. By selecting a proper spectral range enhanced isolated attosecond pulses are generated. We study how dynamic phase matching caused by the interplay among waveguide mode, neutral atomic dispersion, and plasma effect is achieved at the optimal macroscopic conditions, by performing time-frequency analysis and by analyzing the evolution of the driving laser’s electric field during the propagation. Our results, when combined with the on-going push of high-repetition-rate lasers (sub- to few MHz’s) may eventually lead to the generation of high-flux, low-divergence soft X-ray tabletop isolated attosecond pulses for applications.

  18. Highly selective two-color mid-wave and long-wave infrared detector hybrid based on Type-II superlattices.

    PubMed

    Huang, Edward Kwei-wei; Hoang, Minh-Anh; Chen, Guanxi; Ramezani-Darvish, Shaban; Haddadi, Abbas; Razeghi, Manijeh

    2012-11-15

    We report a two-color mid-wave infrared (MWIR) and long-wave infrared (LWIR) co-located detector with 3 μm active region thickness per channel that is highly selective and can perform under high operating temperatures for the MWIR band. Under back-side illumination, a temperature evolution study of the MWIR detector's electro-optical performance found the 300 K background-limit with 2π field-of-view to be achieved below operating temperatures of 160 K, at which the temperature's 50% cutoff wavelength was 5.2 μm. The measured current reached the system limit of 0.1 pA at 110 K for 30 μm pixel-sized diodes. At 77 K, where the LWIR channel operated with a 50% cutoff wavelength at 11.2 μm, an LWIR selectivity of ~17% was achieved in the MWIR wave band between 3 and 4.7 μm, making the detector highly selective.

  19. Kinetic study of terahertz generation based on the interaction of two-color ultra-short laser pulses with molecular hydrogen gas

    SciTech Connect

    Soltani Gishini, M. S.; Ganjovi, A.; Saeed, M.

    2016-06-15

    In this work, using a two dimensional particle in cell-Monte Carlo collision simulation scheme, interaction of two-color ultra-short laser pulses with the molecular hydrogen gas (H{sub 2}) is examined. The operational laser parameters, i.e., its pulse shape, duration, and waist, are changed and, their effects on the density and kinetic energy of generated electrons, THz electric field, intensity, and spectrum are studied. It is seen that the best pulse shape generating the THz signal radiation with the highest intensity is a trapezoidal pulse, and the intensity of generated THz radiation is increased at the higher pulse durations and waists. For all the operational laser parameters, the maximum value of emitted THz signal frequency always remains lower than 5 THz. The intensity of applied laser pulses is taken about 10{sup 14} w/cm{sup 2}, and it is observed that while a small portion of the gaseous media gets ionized, the radiated THz signal is significant.

  20. Phage display evolution of a peptide substrate for yeast biotin ligase and application to two-color quantum dot labeling of cell surface proteins.

    PubMed

    Chen, Irwin; Choi, Yoon-Aa; Ting, Alice Y

    2007-05-23

    Site-specific protein labeling with Escherichia coli biotin ligase (BirA) has been used to introduce fluorophores, quantum dots (QDs), and photocross-linkers onto recombinant proteins fused to a 15-amino acid acceptor peptide (AP) substrate for BirA and expressed on the surface of living mammalian cells. Here, we used phage display to engineer a new and orthogonal biotin ligase-AP pair for site-specific protein labeling. Yeast biotin ligase (yBL) does not recognize the AP, but we discovered a new 15-amino acid substrate for yBL called the yeast acceptor peptide (yAP), using two generations of phage display selection from 15-mer peptide libraries. The yAP is not recognized by BirA, and thus, we were able to specifically label AP and yAP fusion proteins coexpressed in the same cell with differently colored QDs. We fused the yAP to a variety of recombinant proteins and demonstrated biotinylation by yBL at the N-terminus, C-terminus, and within a flexible internal region. yBL is extremely sequence-specific, as endogenous proteins on the surface of yeast and HeLa cells are not biotinylated. This new methodology expands the scope of biotin ligase labeling to two-color imaging and yeast-based applications.

  1. Optimal generation of spatially coherent soft X-ray isolated attosecond pulses in a gas-filled waveguide using two-color synthesized laser pulses

    DOE PAGES

    Jin, Cheng; Hong, Kyung -Han; Lin, C. D.

    2016-12-08

    Here, we numerically demonstrate the generation of intense, low-divergence soft X-ray isolated attosecond pulses in a gas-filled hollow waveguide using synthesized few-cycle two-color laser waveforms. The waveform is a superposition of a fundamental and its second harmonic optimized such that highest harmonic yields are emitted from each atom. We then optimize the gas pressure and the length and radius of the waveguide such that bright coherent high-order harmonics with angular divergence smaller than 1 mrad are generated, for photon energy from the extreme ultraviolet to soft X-rays. By selecting a proper spectral range enhanced isolated attosecond pulses are generated. Wemore » study how dynamic phase matching caused by the interplay among waveguide mode, neutral atomic dispersion, and plasma effect is achieved at the optimal macroscopic conditions, by performing time-frequency analysis and by analyzing the evolution of the driving laser’s electric field during the propagation. Our results, when combined with the on-going push of high-repetition-rate lasers (sub- to few MHz’s) may eventually lead to the generation of high-flux, low-divergence soft X-ray tabletop isolated attosecond pulses for applications.« less

  2. Optimal generation of spatially coherent soft X-ray isolated attosecond pulses in a gas-filled waveguide using two-color synthesized laser pulses

    SciTech Connect

    Jin, Cheng; Hong, Kyung -Han; Lin, C. D.

    2016-12-08

    Here, we numerically demonstrate the generation of intense, low-divergence soft X-ray isolated attosecond pulses in a gas-filled hollow waveguide using synthesized few-cycle two-color laser waveforms. The waveform is a superposition of a fundamental and its second harmonic optimized such that highest harmonic yields are emitted from each atom. We then optimize the gas pressure and the length and radius of the waveguide such that bright coherent high-order harmonics with angular divergence smaller than 1 mrad are generated, for photon energy from the extreme ultraviolet to soft X-rays. By selecting a proper spectral range enhanced isolated attosecond pulses are generated. We study how dynamic phase matching caused by the interplay among waveguide mode, neutral atomic dispersion, and plasma effect is achieved at the optimal macroscopic conditions, by performing time-frequency analysis and by analyzing the evolution of the driving laser’s electric field during the propagation. Our results, when combined with the on-going push of high-repetition-rate lasers (sub- to few MHz’s) may eventually lead to the generation of high-flux, low-divergence soft X-ray tabletop isolated attosecond pulses for applications.

  3. Enhancing nonlinear energy deposition into transparent solids with an elliptically polarized and mid-IR heating laser pulse under two-color femtosecond impact

    NASA Astrophysics Data System (ADS)

    Potemkin, F. V.; Mareev, E. I.; Bezsudnova, Yu I.; Platonenko, V. T.; Bravy, B. G.; Gordienko, V. M.

    2017-06-01

    We report on an enhancement of deposited energy density of up to 10 kJ cm-3 inside transparent solids (fused silica and quartz) from using two-color µJ energy level tightly focused (NA  =  0.5) co-propagating linearly polarized seeding (visible, 0.62 µm) and elliptically polarized heating (near-IR, 1.24 µm) femtosecond laser pulses. The rise in temperature under constant volume causes pressure of up to 12 GPa. It has been shown experimentally and theoretically that the production of seeding electrons through multiphoton ionization by visible laser pulse paves the way for controllability of the energy deposition and laser-induced micromodification via carrier heating by delayed infrared laser pulses inside the material. The developed theoretical approach predicts that the deposited energy density will be enhanced by up to 14 kJ cm-3 when using longer (up to 5 µm) wavelengths for heating laser pulses inside transparent solids.

  4. Hapten-Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX Using Primary Antibodies Raised in the Same Host Species.

    PubMed

    Hagen, Jodi; Schwartz, David; Kalyuzhny, Alexander E

    2017-01-01

    Multiplex staining of cell and tissue sections with antibodies raised in the same host species is a serious challenge because of unwanted but inevitable cross-reactivity of secondary antibodies with irrelevant primary antibodies. Several techniques can be used to overcome this obstacle including direct labeling of primary antibodies with fluorescent tags and using tyramide signal amplification. Unfortunately these techniques either lack sensitivity, or require a long multistep protocol which can cause physical damage of specimens. As an alternative, we have developed a protocol based on conjugation of primary antibodies to small-size hapten molecules which can be detected with hapten-specific fluorescent secondary antibodies. This technique has been used for two-color labeling of Y845 phosphorylated Epidermal Growth Factor Receptor (EGFR) and S139 phosphorylated histone H2AX protein in A431 human epidermoid carcinoma cells. Our novel hapten-anti-hapten detection chemistry allows for generating a stronger fluorescent signal and completely avoid cross-interactions of secondary antibodies with irrelevant primary antibodies.

  5. The mechanics of unrest at Long Valley caldera, California: 1. Modeling the geometry of the source using GPS, leveling and two-color EDM data

    NASA Astrophysics Data System (ADS)

    Battaglia, M.; Segall, P.; Murray, J.; Cervelli, P.; Langbein, J.

    2003-10-01

    We surveyed 44 existing leveling monuments in Long Valley caldera in July 1999, using dual frequency global positioning system (GPS) receivers. We have been able to tie GPS and leveling to a common reference frame in the Long Valley area and computed the vertical deformation by differencing GPS-based and leveled orthometric heights. The resurgent dome uplifted 74±7 cm from 1975 to 1999. To define the inflation source, we invert two-color EDM and uplift data from the 1985-1999 unrest period using spherical or ellipsoidal sources. We find that the ellipsoidal source satisfies both the vertical and horizontal deformation data, whereas the spherical point source cannot. According to our analysis of the 1985-1999 data, the main source of deformation is a prolate ellipsoid located beneath the resurgent dome at a depth of 5.9 km (95% bounds of 4.9-7.5 km). This body is vertically elongated, has an aspect ratio of 0.475 (95% bounds are 0.25-0.65) and a volume change of 0.086 km 3 (95% bounds are 0.06-0.13 km 3). Failure to account for the ellipsoidal nature of the source biases the estimated source depth by 2.1 km (35%), and the source volume by 0.038 km 3 (44%).

  6. Controlling the contributions to high-order harmonic generation from different nuclei of N2 with an orthogonally polarized two-color laser field

    NASA Astrophysics Data System (ADS)

    Du, Hui; Pan, Xue-Fei; Liu, Hai-Feng; Zhang, Hong-Dan; Zhang, Jun; Guo, Jing; Liu, Xue-Shen

    2016-09-01

    The generation of high-order harmonic and the attosecond pulse of the N2 molecule with an orthogonally polarized two-color laser field are investigated by the strong-field Lewenstein model. We show that the control of contributions to high-order harmonic generation (HHG) from different nuclei is realized by properly selecting the relative phase. When the relative phase is chosen to be φ = 0.4π, the contribution to HHG from one nucleus is much more than that from another. Interference between two nuclei can be suppressed greatly; a supercontinuum spectrum of HHG appears from 40 eV to 125 eV. The underlying physical mechanism is well explained by the time-frequency analysis and the semi-classical three-step model with a finite initial transverse velocity. By superposing several orders of harmonics, an isolated attosecond pulse with a duration of 80 as can be generated. Project supported by the National Natural Science Foundation of China (Grant Nos. 11271158, 61575077, and 11574117).

  7. Variations in fault slip and strain accumulation at Parkfield, California: initial results using two-color geodimeter measurements 1984-1988

    USGS Publications Warehouse

    Langbein, J.O.; Burford, R.O.; Slater, L.E.

    1990-01-01

    Repeated length measurements of several geodetic baselines near Parkfield, California, have revealed significant variations in the local rates of shallow fault slip and strain. This network of baselines, surveyed several times each week, straddles the San Andreas fault in the transition zone between the creeping section to the northwest and the locked section to the southeast. Principal mode analysis of the length change data indicates that the two largest components of the signal are (1) secular extensions and contractions consistent with surface slip on the main strand of the San Andreas fault, and (2) a large seasonal oscillation with no obvious spatial coherence. On most of these baselines, the second component appears to be in phase with seasonal rainfall. An interesting conclusion from the two-color data is that surface slip on the San Andreas fault appears to be spread over a 2-km-wide zone on the south flank of the Middle Mountain but is confined to a very narrow zone to the south as the fault passes through the center of the network. -from Authors

  8. Prescribed 3-D Direct Writing of Suspended Micron/Sub-micron Scale Fiber Structures via a Robotic Dispensing System.

    PubMed

    Yuan, Hanwen; Cambron, Scott D; Keynton, Robert S

    2015-06-12

    A 3-axis dispensing system is utilized to control the initiating and terminating fiber positions and trajectory via the dispensing software. The polymer fiber length and orientation is defined by the spatial positioning of the dispensing system 3-axis stages. The fiber diameter is defined by the prescribed dispense time of the dispensing system valve, the feed rate (the speed at which the stage traverses from an initiating to a terminating position), the gauge diameter of the dispensing tip, the viscosity and surface tension of the polymer solution, and the programmed drawing length. The stage feed rate affects the polymer solution's evaporation rate and capillary breakup of the filaments. The dispensing system consists of a pneumatic valve controller, a droplet-dispensing valve and a dispensing tip. Characterization of the direct write process to determine the optimum combination of factors leads to repeatedly acquiring the desired range of fiber diameters. The advantage of this robotic dispensing system is the ease of obtaining a precise range of micron/sub-micron fibers onto a desired, programmed location via automated process control. Here, the discussed self-assembled micron/sub-micron scale 3D structures have been employed to fabricate suspended structures to create micron/sub-micron fluidic devices and bioengineered scaffolds.

  9. Mechanisms underlying the vasorelaxant action of the pimarane ent-8(14),15-pimaradien-3beta-ol in the isolated rat aorta.

    PubMed

    Hipólito, Ulisses V; Rodrigues, Gerson J; Lunardi, Claure N; Bonaventura, Daniella; Ambrosio, Sergio R; de Oliveira, Ana M; Bendhack, Lusiane M; da Costa, Fernando B; Tirapelli, Carlos R

    2009-08-15

    Pimarane-type diterpenes were described to exert antispasmodic and relaxant activities. Based on this observation we hypothesized that the diterpene ent-8(14),15-pimaradien-3beta-ol (PA-3beta-ol) induced vascular relaxation. With this purpose, the present work investigates the mechanisms involved in the vasorelaxant effect of the pimarane-type diterpene PA-3beta-ol. Vascular reactivity experiments, using standard muscle bath procedures, were performed in isolated aortic rings from male Wistar rats. Cytosolic calcium concentration ([Ca(2+)]c) was measured by confocal microscopy using the fluorescent probe Fluo-3AM. PA-3beta-ol (10, 50 and 100 micromol/l) inhibited phenylephrine and KCl-induced contraction in either endothelium-intact or denuded rat aortic rings. PA-3beta-ol also reduced CaCl(2)-induced contraction in Ca(2+)-free solution containing KCl (30 mmol/l) or phenylephrine (0.1 micromol/l). PA-3beta-ol (1-300 micromol/l) concentration dependently relaxed phenylephrine-pre-contracted rings with intact or denuded endothelium. The diterpene also relaxed KCl-pre-contracted rings with intact or denuded endothelium. Moreover, Ca(2+) mobilization study showed that PA-3beta-ol (100 micromol/l) and verapamil (1 micromol/l) inhibited the increase in Ca(2+)-concentration in smooth muscle and endothelial cells induced by phenylephrine (10 micromol/l) or KCl (60 mmol/l). Pre-incubation of intact or denuded aortic rings with N(G)-nitro-l-arginine methyl ester (L-NAME, 100 micromol/l) and 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ, 1 micromol/l) produced a rightward displacement of the PA-3beta-ol concentration-response curves. On the other hand, 7-nitroindazole (100 micromol/l), 1400 W (1 micromol/l), indomethacin (10 micromol/l) and tetraethylammonium (1 mmol/l) did not affect PA-3beta-ol-induced relaxation. Collectively, our results provide evidence that the effects elicited by PA-3beta-ol involve extracellular Ca(2+) influx blockade. Its effects are also partly

  10. Post-Hurricane Ike coastal oblique aerial photographs collected along the Alabama, Mississippi, and Louisiana barrier islands and the north Texas coast, September 14-15, 2008

    USGS Publications Warehouse

    Morgan, Karen L. M.; Krohn, M. Dennis; Guy, Kristy K.

    2016-04-28

    The U.S. Geological Survey (USGS), as part of the National Assessment of Coastal Change Hazards project, conducts baseline and storm-response photography missions to document and understand the changes in vulnerability of the Nation's coasts to extreme storms (Morgan, 2009). On September 14-15, 2008, the USGS conducted an oblique aerial photographic survey along the Alabama, Mississippi, and Louisiana barrier islands and the north Texas coast, aboard a Beechcraft Super King Air 200 (aircraft) at an altitude of 500 feet (ft) and approximately 1,200 ft offshore. This mission was flown to collect post-Hurricane Ike data for assessing incremental changes in the beach and nearshore area since the last survey, flown on September 9-10, 2008, and the data can be used in the assessment of future coastal change.The photographs provided in this report are Joint Photographic Experts Group (JPEG) images. ExifTool was used to add the following to the header of each photo: time of collection, Global Positioning System (GPS) latitude, GPS longitude, keywords, credit, artist (photographer), caption, copyright, and contact information. The photograph locations are an estimate of the position of the aircraft at the time the photograph was taken and do not indicate the location of any feature in the images (see the Navigation Data page). These photographs document the state of the barrier islands and other coastal features at the time of the survey. Pages containing thumbnail images of the photographs, referred to as contact sheets, were created in 5-minute segments of flight time. These segments can be found on the Photos and Maps page. Photographs can be opened directly with any JPEG-compatible image viewer by clicking on a thumbnail on the contact sheet.In addition to the photographs, a Google Earth Keyhole Markup Language (KML) file is provided and can be used to view the images by clicking on the marker and then clicking on either the thumbnail or the link above the thumbnail

  11. Visibility related to backscatter at 1.54 micron

    NASA Technical Reports Server (NTRS)

    Barber, T. L.; Larson, D. R.

    1986-01-01

    The lidar process was shown to have the necessary potential to fulfill the need for a remote measurement of visibility. Visibility can be inferred from a lidar return optical extinction. The wavelength 1.54 micron was chosen, being near the visible wavelength region and having a high eye safety threshol, 200,000 times higher than 1.06 micron; 1.54 is the erbium laser wavelength. This research utilized 105 measured height profiles of natural droplet size distributions data, taken in clouds, fog, and haze. These profiles were examined to determine the completeness of the droplet counting data. It was found that the particle spectrometer data were incomplete in the very light ford and haze so this portion of the data was eliminated. Utilizing the Mie theory, these droplet size distribution profiles were converted to backscatter at 1.54 micron and extinction in the visible region, 0.55 micron. Using Koschmeider's relationship, the extinction profiles were converted to visibility. The visibility and backscatter profiles were compared to develop a relationship between visibility and backscatter at 1.54 micron.

  12. Photodecomposition profiles of beta-bond cleavage of phenylphenacyl derivatives in the higher triplet excited states during stepwise two-color two-laser flash photolysis.

    PubMed

    Yamaji, Minoru; Cai, Xichen; Sakamoto, Masanori; Fujitsuka, Mamoru; Majima, Tetsuro

    2008-11-13

    Photochemical properties of p-phenylphenacyl derivatives (PP-X) having C-halide, C-S, and C-O bonds in the lowest (T 1) and higher (T n ) triplet excited states were investigated in solution by using single-color and stepwise two-color two-laser flash photolysis techniques. PP-Xs (X = Br, SH, and SPh) undergo beta-bond dissociation in the lowest singlet excited states (S 1) while the C-X bonds of other PP-Xs are stable upon 266-nm laser photolysis. The T 1(pi,pi*) states of PP-X were efficiently produced during 355-nm laser photolysis of benzophenone as a triplet sensitizer. Triplet PP-Xs deactivate to the ground state without photochemical reactions. Upon 430-nm laser photolysis of the T 1 states of PP-X (X = Br, Cl, SH, SPh, OH, OMe, and OPh), decomposition of PP-X in the T n states was found. On the basis of the changes in the transient absorption, quantum yields (Phi dec) of the decomposition of PP-X in the T n states were determined, while bond dissociation energies (BDE) of the C-X bonds were calculated by computations. According to the relationship between the Phi dec and BDE values, it was shown that the decomposition of PP-X in the T n state is due to beta-cleavage of the corresponding C-X bond, and that the state energy of the reactive T n for the C-O bond cleavage differs from that for the C-halide and C-S bond cleavage. The reaction profiles of the C-X bond cleavage of PP-X in the T n states were discussed.

  13. Fully automated sample preparation microsystem for genetic testing of hereditary hearing loss using two-color multiplex allele-specific PCR.

    PubMed

    Zhuang, Bin; Gan, Wupeng; Wang, Shuaiqin; Han, Junping; Xiang, Guangxin; Li, Cai-Xia; Sun, Jing; Liu, Peng

    2015-01-20

    A fully automated microsystem consisting of a disposable DNA extraction and PCR microchip, as well as a compact control instrument, has been successfully developed for genetic testing of hereditary hearing loss from human whole blood. DNA extraction and PCR were integrated into a single 15-μL reaction chamber, where a piece of filter paper was embedded for capturing genomic DNA, followed by in-situ PCR amplification without elution. Diaphragm microvalves actuated by external solenoids together with a "one-way" fluidic control strategy operated by a modular valve positioner and a syringe pump were employed to control the fluids and to seal the chamber during thermal cycling. Fully automated DNA extractions from as low as 0.3-μL human whole blood followed by amplifications of 59-bp β-actin fragments can be completed on the microsystem in about 100 min. Negative control tests that were performed between blood sample analyses proved the successful elimination of any contamination or carryover in the system. To more critically test the microsystem, a two-color multiplex allele-specific PCR (ASPCR) assay for detecting c.176_191del16, c.235delC, and c.299_300delAT mutations in GJB2 gene that accounts for hereditary hearing loss was constructed. Two allele-specific primers, one labeled with TAMRA for wild type and the other with FAM for mutation, were designed for each locus. DNA extraction from blood and ASPCR were performed on the microsystem, followed by an electrophoretic analysis on a portable microchip capillary electrophoresis system. Blood samples from a healthy donor and five persons with genetic mutations were all accurately analyzed with only two steps in less than 2 h.

  14. The Beauty and Limitations of 10 Micron Heterodyne Interferometry (ISI)

    NASA Technical Reports Server (NTRS)

    Danchi, William C.

    2003-01-01

    Until recently, heterodyne interferometry at 10 microns has been the only successful technique for stellar interferometry in the very difficult atmospheric window from 9-12 microns. For most of its operational lifetime the U.C. Berkeley Infrared Spatial Interferometer was a single-baseline two telescope (1.65 m aperture) system using CO2 lasers as local oscillators. This instrument was designed and constructed from 1983-1988, and first fringes were obtained at Mt. Wilson in June 1988. During the past few years, a third telescope was constructed and just recently the first closure phases were obtained at 11.15 microns. We discuss the history, physics and technology of heterodyne interferometry in the mid-infrared, and some key astronomical results that have come from this unique instrument.

  15. Influence of the Efavirenz Micronization on Tableting and Dissolution

    PubMed Central

    Pinto, Eduardo Costa; do Carmo, Flávia Almada; da Silva Honório, Thiago; da Silva Ascenção Barros, Rita de Cássia; Castro, Helena Carla Rangel; Rodrigues, Carlos Rangel; Esteves, Valéria Sant'Anna Dantas; Rocha, Helvécio Vinícius Antunes; de Sousa, Valeria Pereira; Cabral, Lucio Mendes

    2012-01-01

    The purpose of this study was to propose an analytical procedure that provides the effects of particle size and surface area on dissolution of efavirenz. Five different batches obtained by different micronization processes and with different particle size distribution and surface area were studied. The preformulation studies and dissolution curves were used to confirm the particle size distribution effect on drug solubility. No polymorphic variety or amorphization was observed in the tested batches and the particle size distribution was determined as directly responsible for the improvement of drug dissolution. The influence of the preparation process on the tablets derived from efavirenz was observed in the final dissolution result in which agglomeration, usually seen in non-lipophilic micronized material, was avoided through the use of an appropriate wet granulation method. For these reasons, micronization may represent one viable alternative for the formulation of brick dust drugs. PMID:24300301

  16. MSX Observations of the Eclipsed Moon at 4 Microns

    NASA Astrophysics Data System (ADS)

    Howard, J. W.; Little, S. J.; Murdock, T. L.

    1997-07-01

    The lunar eclipse of September 27, 1996 presented the opportunity to observe the 4 micron emission from the moon during totality. The Midcourse Space Experiment (MSX) satellite made observations three times during the totality phase of the eclipse. These observations in Bands B1 (4.22 - 4.36 microns) and B2 (4.24 - 4.45 microns) were used to construct images of the eclipsed moon. The images have been analyzed for temperature and location of thermal anomalies on the moon as well as for temperatures of extended maria and highland areas. Maps of the moon to illustrate the location and brightness of thermal anomalies first seen by Saari and Shorthill (1965) and temperature comparisons with microwave measurements of selected regions on the moon (Sandor and Clancy, 1995) will be made. References: Saari, J. M., and R. W. Shorthill, 1965, Nature, 205, p. 964. Sandor, Brad J., and R. Todd Clancy, 1995, Icarus, 115, p. 387.

  17. Structure in the nucleus of NGC 1068 at 10 microns

    NASA Technical Reports Server (NTRS)

    Tresch-Fienberg, R.; Fazio, G. G.; Gezari, D. Y.; Hoffmann, W. F.; Lamb, G. M.; Shu, P. K.; Mccreight, C. R.

    1987-01-01

    New 8 to 13 micron array camera images of the central kiloparsec of Seyfert 2 galaxy NGC 1068 resolve structure that is similar to that observed at visible and radio wavelengths. The images reveal an infrared source which is extended and asymmetric, with its long axis oriented at P.A. 33 deg. Maps of the spatial distribution of 8 to 13 micron color temperature and warm dust opacity are derived from the multiwavelength infrared images. The results suggest that there exist two pointlike luminosity sources in the central regions of NGC 1068, with the brighter source at the nucleus and the fainter one some 100 pc to the northeast. This geometry strengthens the possibility that the 10 micron emission observed from grains in the nucleus is powered by a nonthermal source. In the context of earlier visible and radio studies, these results considerably strengthen the case for jet induced star formation in NGC 1068.

  18. Cooled grating array spectrometer for 0.6-5 microns

    NASA Technical Reports Server (NTRS)

    Nordholt, J. E.; Lacy, J. H.

    1982-01-01

    A grating spectrometer, designed to illuminate an array of 122 InSb photodiodes with minimum aberrations and maximum speed, has been constructed. The instrument will be used on the 5 meter Hale telescope at Palomar Observatory, and is easily adaptable to telescopes of various focal ratios. A resolving power of 100-1000 can be obtained at wavelengths between 0.6 microns and 5 microns with remotely interchangeable gratings. The spectrometer is sufficiently compact to fit on the 8-inch work surface of a commercially available dewar, and uses simple on-axis spherical and paraboloidal optical elements. The camera mirror produces an f/2.5 beam which, with the 0.2 mm detectors, allows a 3-in. focal-plane aperture on the 5 meter telescope. All rays fall within a 100 microns blur circle at all points along the array. Distortions have been corrected with a tilted field flattening lens in front of the detector.

  19. Metallization technology for tenth-micron range integrated circuits

    SciTech Connect

    Berry, L.A.; Harper, M.E.

    1996-11-27

    A critical step in the fabrication of integrated circuits is the deposition of metal layers which interconnect the various circuit elements that have been formed in earlier process steps. In particular, columns of copper several times higher than the characteristic dimension of the circuit elements was needed. Features with a diameter of a few tenths of a micron and a height of about one micron need to be filled at rates in the half to one micron per minute range. With the successful development of a copper deposition technology meeting these requirements, integrated circuits with simpler designs and higher performance could be economically manufactured. Several technologies for depositing copper were under development. No single approach had an optimum combination of performance (feature characteristics), cost (deposition rates), and manufacturability (integration with other processes and tool reliability). Chemical vapor deposition, plating, sputtering and ionized-physical vapor deposition (I-PVD) were all candidate technologies. Within this project, the focus was on I-PVD.

  20. 2--14 microns Spectroscopy of Vega-type Stars

    NASA Astrophysics Data System (ADS)

    Fajardo-Acosta, S. B.; Knacke, R. F.; Hackwell, J. A.; Lynch, D. K.; Russell, R. W.; Hanner, M. S.

    1994-12-01

    We present intermediate-resolution (lambda /Delta lambda ~ 50) infrared (2--14 microns) spectroscopy of four early-type main-sequence stars, conducted with the Aerospace Corp. Infrared Spectrograph. We observed beta UMa (A1 V), alpha Aql (A7 V), and beta Leo (A3 V) at the 1.3-m KPNO telescope in May 1993, and zeta Lep (A2 V) at the 3.0-m IRTF telescope in Nov. 1993. The Vega-type stars beta UMa and zeta Lep showed weak but definite excess flux at ~ 10 microns in previous groundbased photometric surveys (Fajardo-Acosta, Telesco & Knacke 1994, in preparation; Aumann & Probst 1991, ApJ, 368, 264). We observed alpha Aql and beta Leo to confirm that their ~ 10 microns spectra do not show any excess. The weak ~ 10 microns excess features in our spectra of beta UMa and zeta Lep are probably indicative of large grains and/or a small quantity of dust around these stars. Their weak features contrast with the prominent silicate emission feature previously seen in beta Pic and 51 Oph. The grains are hotter in zeta Lep than in beta UMa, as indicated by an excess already present at short wavelengths ( ~ 8.5 microns) in the spectrum of the former, as opposed to the 10--11 microns excess of the latter. Dust around these two stars could be an assemblage of amorphous minerals, probably of a variety of sizes, as suggested by their broad features. We compared the excess spectra of zeta Lep and beta UMa with those of comets (reviewed by Hanner, Lynch, & Russell 1994, ApJ, 425, 274) and found they resemble those of dust-poor comets such as Austin 1990 V and Okazaki-Levy-Rudenko 1989 XIX.

  1. Optically controlled grippers for manipulating micron-sized particles

    NASA Astrophysics Data System (ADS)

    Gibson, Graham; Barron, Louise; Beck, Fiona; Whyte, Graeme; Padgett, Miles

    2007-01-01

    We report the development of a joystick controlled gripper for the real-time manipulation of micron-sized objects, driven using holographic optical tweezers (HOTs). The gripper consists of an arrangement of four silica beads, located in optical traps, which can be positioned and scaled in order to trap an object indirectly. The joystick can be used to grasp, move (lateral or axial), and change the orientation of the target object. The ability to trap objects indirectly allows us to demonstrate the manipulation of a strongly scattering micron-sized metallic particle.

  2. Characterization of the 1 ^5Πu - 1 ^5Πg Band of C_2 by Two-Color Resonant Four-Wave Mixing and Lif

    NASA Astrophysics Data System (ADS)

    Radi, Peter

    2015-06-01

    The application of two-color resonant four-wave mixing (TC-RFWM) in combination with a discharge slit-source in a molecular beam environment is advantageous for the study of perturbations in C_2. Initial investigations have shown the potential of the method by a detailed deperturbation of the d3Π_g, v=4 state. The deperturbation of the d3Π_g, v=6 state unveiled the presence of the energetically lowest high-spin state of C_2. This dark state gains transition strength through the perturbation process with the d3Π_g, v=6 state yielding weak spectral features that are observable by the high sensitivity of the TC-RFWM technique. The successful deperturbation study of the d3Π_g, v=6 state resulted in the spectroscopic characterization of the quintet (15Πg) and an additional triplet state (d3Π_g, v=19). More recently, investigations have been performed by applying unfolded TC-RFWM to obtain further information on the quintet manifold. The first high-spin transition (15Πu) - 15Πg)) has been observed via an intermediate ``gateway'' state exhibiting both substantial triplet and quintet character owing to the perturbation between the 15Πg), v=0 and the d3Π_g, v=6 states. The high-lying quintet state is found to be predissociative and displays a shallow potential that accommodates three vibrational levels only. Further studies of the high-spin system will be presented in this contribution. By applying TC-RFWM and laser-induced fluorescence, data on the vibrational structure of the 15Πu - 15Πg system is obtained. The results are combined with high-level ab initio computations at the multi-reference configuration interaction (MRCI) level of theory and the largest possible basis currently implemented in the 2012 version of MOLPRO. P. Bornhauser, G. Knopp, T. Gerber, and P.P. Radi, Journal of Molecular Spectroscopy 262, 69 (2010) P. Bornhauser, Y. Sych, G. Knopp, T. Gerber, and P.P. Radi, Journal of Chemical Physics 134, 044302 (2011) Bornhauser, P., Marquardt, R

  3. Two-color multiparametric method for flow cytometric DNA analysis of carcinomas using staining for cytokeratin and leukocyte-common antigen.

    PubMed

    Zarbo, R J; Visscher, D W; Crissman, J D

    1989-12-01

    Solid tumors contain heterogenous cell populations, resulting in flow cytometric (FCM) DNA quantitations of a mixture of tumor and host cells. Such mixed populations can result in dilution of the tumor cells by the host cells, in difficulty defining the diploid reference mean and in histogram peak overlap, precluding cell-cycle analysis. In this study, epithelial (tumor) cells and contaminating host cells in 100 consecutively accessioned human mammary and colorectal carcinomas were segregated in a multiparametric two-color FCM DNA analysis of intact, ethanol-fixed cells. These two carcinomas and bladder carcinomas contain a cytoskeleton of simple epithelium that is selectively stained with an FITC-labeled monoclonal antibody (MAb) to cytokeratin (CK: CAM 5.2-FITC). This MAb detects the CK 8, CK 18 and CK 19 consistently present in all layers of normal and neoplastic urothelium, colonic epithelium and mammary epithelium. Gating on CK in these tumors enables the nonstaining leukocytes, stromal fibroblasts and endothelial cells to be excluded from DNA analysis. A separate aliquot of each tumor evaluated was labeled with an MAb to leukocyte-common antigen (LCA-FITC) to serve as a patient-specific intrinsic diploid reference standard. Both the CK-labeled and LCA-labeled cells were then dual labeled for DNA with propidium iodide. This method (1) correctly identified the intrinsic diploid (LCA-positive) channel, allowing an accurate definition of normal cell DNA content for calculation of the DNA index; and (2) resulted in an increased sensitivity in the identification of both diploid and abnormal hyperdiploid tumor cell populations. It also (3) limited DNA cell cycle analysis to urothelial, colonic and mammary epithelial cells, the majority of which were neoplastic in carefully selected tumor samples. In addition, this method (4) clarified near-tetraploid populations that overlap the normal nonepithelial G2M region by diminishing the normal G2M peak and accentuating the

  4. Validar: a testbed for advanced 2-micron Doppler lidar

    NASA Astrophysics Data System (ADS)

    Koch, Grady J.; Petros, Mulugeta; Barnes, Bruce W.; Beyon, Jeffrey Y.; Amzajerdian, Farzin; Yu, Jirong; Kavaya, Michael J.; Singh, Upendra N.

    2004-09-01

    High-energy 2-micron lasers have been incorporated in a breadboard coherent Doppler lidar to test component technologies and explore applications for remote sensing of the atmosphere. Design of the lidar is presented including aspects in the laser transmitter, receiver, photodetector, and signal processing. Sample data is presented on wind profiling and CO2 concentration measurements.

  5. Improved whisker pointing technique for micron-size diode contact

    NASA Technical Reports Server (NTRS)

    Mattauch, R. J.; Green, G.

    1982-01-01

    Pointed phosphor-bronze whiskers are commonly used to contact micron-size Schottky barrier diodes. A process is presented which allows pointing such wire and achieving the desired cone angle and tip diameter without the use of highly undesirable chemical reagents.

  6. An Advanced Wafer Stepper For Sub-Micron Fabrication

    NASA Astrophysics Data System (ADS)

    Mayer, Herbert E.; Loebach, Ernst W.

    1987-09-01

    An advanced wafer stepper is presented addressing the specific problems involved by sub-micron lithography such as alignment and focusing to multilayer resist films. New sub-systems were developed while maintaining principles well proven in a previous design. The system is described emphasizing the new sub-systems, and performance data are presented.

  7. Erratum: [O I] 63 Micron Absorption in NGC 6334

    NASA Astrophysics Data System (ADS)

    Kraemer, Kathleen E.; Jackson, James M.; Lane, Adair P.

    1998-12-01

    In the paper ``[O I] 63 Micron Absorption in NGC 6334'' by Kathleen E. Kraemer, James M. Jackson, and Adair P. Lane (ApJ, 503, 785 [1998]), Figure 2 was printed as the negative of the original figure as the result of an error in the printing process. The correct version of Figure 2 appears below. The Press sincerely regrets this error.

  8. Vacuum probe sampler removes micron-sized particles from surfaces

    NASA Technical Reports Server (NTRS)

    Whitfield, W. J.

    1968-01-01

    Vacuum probe sampler removes micron-sized particles from sensitive surfaces, without damage to the surface. The probe has a critical orifice to ensure an optimum airflow rate that disturbs the boundary layer of air and raises bacteria from the surface into the probe with the moving air stream.

  9. Micron-gap thermophotovoltaic systems enhanced by nanowires

    NASA Astrophysics Data System (ADS)

    Mirmoosa, Mohammad Sajjad; Simovski, Constantin

    2015-01-01

    We introduce new micron-gap thermophotovoltaic systems enhanced by tungsten nanowires. We theoretically show that these systems allow the frequency-selective super-Planckian spectrum of radiative heat transfer that promises a very efficient generation of electricity. Our system analysis covers practical aspects such as output power per unit area and efficiency of the tap water cooling.

  10. The NASA - Arc 10/20 micron camera

    NASA Technical Reports Server (NTRS)

    Roellig, T. L.; Cooper, R.; Deutsch, L. K.; Mccreight, C.; Mckelvey, M.; Pendleton, Y. J.; Witteborn, F. C.; Yuen, L.; Mcmahon, T.; Werner, M. W.

    1994-01-01

    A new infrared camera (AIR Camera) has been developed at NASA - Ames Research Center for observations from ground-based telescopes. The heart of the camera is a Hughes 58 x 62 pixel Arsenic-doped Silicon detector array that has the spectral sensitivity range to allow observations in both the 10 and 20 micron atmospheric windows.

  11. Biogeography of a human oral microbiome at the micron scale

    PubMed Central

    Mark Welch, Jessica L.; Rossetti, Blair J.; Rieken, Christopher W.; Dewhirst, Floyd E.; Borisy, Gary G.

    2016-01-01

    The spatial organization of complex natural microbiomes is critical to understanding the interactions of the individual taxa that comprise a community. Although the revolution in DNA sequencing has provided an abundance of genomic-level information, the biogeography of microbiomes is almost entirely uncharted at the micron scale. Using spectral imaging fluorescence in situ hybridization as guided by metagenomic sequence analysis, we have discovered a distinctive, multigenus consortium in the microbiome of supragingival dental plaque. The consortium consists of a radially arranged, nine-taxon structure organized around cells of filamentous corynebacteria. The consortium ranges in size from a few tens to a few hundreds of microns in radius and is spatially differentiated. Within the structure, individual taxa are localized at the micron scale in ways suggestive of their functional niche in the consortium. For example, anaerobic taxa tend to be in the interior, whereas facultative or obligate aerobes tend to be at the periphery of the consortium. Consumers and producers of certain metabolites, such as lactate, tend to be near each other. Based on our observations and the literature, we propose a model for plaque microbiome development and maintenance consistent with known metabolic, adherence, and environmental considerations. The consortium illustrates how complex structural organization can emerge from the micron-scale interactions of its constituent organisms. The understanding that plaque community organization is an emergent phenomenon offers a perspective that is general in nature and applicable to other microbiomes. PMID:26811460

  12. Release of Micronized Copper Particles from Pressure Treated Wood Products

    EPA Science Inventory

    Micronized copper pressure treated lumber (PTL) has recently been introduced to the consumer market as a replacement for ionized copper PTL. The presence of particulate rather than aqueous copper raises concerns about the exposure of humans as well as the environment to the parti...

  13. Improved whisker pointing technique for micron-size diode contact

    NASA Technical Reports Server (NTRS)

    Mattauch, R. J.; Green, G.

    1982-01-01

    Pointed phosphor-bronze whiskers are commonly used to contact micron-size Schottky barrier diodes. A process is presented which allows pointing such wire and achieving the desired cone angle and tip diameter without the use of highly undesirable chemical reagents.

  14. The NASA - Arc 10/20 micron camera

    NASA Technical Reports Server (NTRS)

    Roellig, T. L.; Cooper, R.; Deutsch, L. K.; Mccreight, C.; Mckelvey, M.; Pendleton, Y. J.; Witteborn, F. C.; Yuen, L.; Mcmahon, T.; Werner, M. W.

    1994-01-01

    A new infrared camera (AIR Camera) has been developed at NASA - Ames Research Center for observations from ground-based telescopes. The heart of the camera is a Hughes 58 x 62 pixel Arsenic-doped Silicon detector array that has the spectral sensitivity range to allow observations in both the 10 and 20 micron atmospheric windows.

  15. The 100 micron detector development program. [gallium doped germanium photoconductors

    NASA Technical Reports Server (NTRS)

    Moore, W. J.

    1976-01-01

    An effort to optimize gallium-doped germanium photoconductors (Ge:Ga) for use in space for sensitive detection of far infrared radiation in the 100 micron region is described as well as the development of cryogenic apparatus capable of calibrating detectors under low background conditions.

  16. Advancement in 17-micron pixel pitch uncooled focal plane arrays

    NASA Astrophysics Data System (ADS)

    Li, Chuan; Skidmore, George; Howard, Christopher; Clarke, Elwood; Han, C. J.

    2009-05-01

    This paper provides an update of 17 micron pixel pitch uncooled microbolometer development at DRS. Since the introduction of 17 micron pitch 640x480 focal plane arrays (FPAs) in 2006, significant progress has been made in sensor performance and manufacturing processes. The FPAs are now in initial production with an FPA noise equivalent temperature difference (NETD), detector thermal time constant, and pixel operability equivalent or better than that of the current 25 micron pixel pitch production FPAs. NETD improvement was achieved without compromising detector thermal response or thermal time constant by simultaneous reduction in bolometer heat capacity and thermal conductance. In addition, the DRS unique "umbrella" microbolometer cavities were optically tuned to optimize detector radiation absorption for specific spectral band applications. The 17 micron pixel pitch FPAs are currently being considered for the next generation soldier systems such as thermal weapon sights (TWS), vehicle driver vision enhancers (DVE), digitally fused enhanced night vision goggles (DENVG) and unmanned air vehicle (UAV) surveillance sensors, because of overall thermal imaging system size, weight and power advantages.

  17. Release of Micronized Copper Particles from Pressure Treated Wood Products

    EPA Science Inventory

    Micronized copper pressure treated lumber (PTL) has recently been introduced to the consumer market as a replacement for ionized copper PTL. The presence of particulate rather than aqueous copper raises concerns about the exposure of humans as well as the environment to the parti...

  18. Validar: A Testbed for Advanced 2-Micron Doppler Lidar

    NASA Technical Reports Server (NTRS)

    Koch, Grady J.; Petros, Mulugeta; Barnes, Bruce W.; Beyon, Jeffrey Y.; Amzajerdian, Farzin; Yu, Jirong; Kavaya, Michael J.; Singh, Upendra N.

    2004-01-01

    High-energy 2-microns lasers have been incorporated in a breadboard coherent Doppler lidar to test component technologies and explore applications for remote sensing of the atmosphere. Design of the lidar is presented including aspects in the laser transmitter, receiver, photodetector, and signal processing. Sample data is presented on wind profiling and CO2 concentration measurements.

  19. Variability of Jupiter's Five-Micron Hot Spot Inventory

    NASA Technical Reports Server (NTRS)

    Yanamandra-Fisher, Padma A.; Orton, G. S.; Wakefield, L.; Rogers, J. H.; Simon-Miller, A. A.; Boydstun, K.

    2012-01-01

    Global upheavals on Jupiter involve changes in the albedo of entire axisymmetric regions, lasting several years, with the last two occurring in 1989 and 2006. Against this backdrop of planetary-scale changes, discrete features such as the Great Red Spot (GRS), and other vortices exhibit changes on shorter spatial- and time-scales. We track the variability of the discrete equatorial 5-micron hot spots, semi-evenly spaced in longitude and confined to a narrow latitude band centered at 6.5degN (southern edge of the North Equatorial Belt, NEB), abundant in Voyager images. Tantalizingly similar patterns were observed in the visible (bright plumes and blue-gray regions), where reflectivity in the red is anti-correlated with 5-microns thermal radiance. Ortiz et al. (1998, GRL, 103) characterized the latitude and drift rates of the hot spots, including the descent of the Galileo probe at the southern edge of a 5-micron hot spot, as the superposition of equatorial Rossby waves, with phase speeds between 99 - 103m/s, relative to System III. We note that the high 5-micron radiances correlate well but not perfectly with high 8.57-micron radiances. Because the latter are modulated primarily by changes in the upper ammonia (NH3) ice cloud opacity, this correlation implies that changes in the ammonia ice cloud field may be responsible for the variability seen in the 5-m maps. During the NEB fade (2011 - early 2012), however, these otherwise ubiquitous features were absent, an atmospheric state not seen in decades. The ongoing NEB revival indicates nascent 5-m hot spots as early as April 2012, with corresponding visible dark spots. Their continuing growth through July 2012 indicates the possit.le re-establishment of Rossby waves. The South Equatorial Belt (SEB) and NEB revivals began similarly with an instability that developed into a major outbreak, and many similarities in the observed propagation of clear regions.

  20. Variability of Jupiter's Five-Micron Hot Spot Inventory

    NASA Technical Reports Server (NTRS)

    Yanamandra-Fisher, Padma A.; Orton, G. S.; Wakefield, L.; Rogers, J. H.; Simon-Miller, A. A.; Boydstun, K.

    2012-01-01

    Global upheavals on Jupiter involve changes in the albedo of entire axisymmetric regions, lasting several years, with the last two occurring in 1989 and 2006. Against this backdrop of planetary-scale changes, discrete features such as the Great Red Spot (GRS), and other vortices exhibit changes on shorter spatial- and time-scales. We track the variability of the discrete equatorial 5-micron hot spots, semi-evenly spaced in longitude and confined to a narrow latitude band centered at 6.5degN (southern edge of the North Equatorial Belt, NEB), abundant in Voyager images. Tantalizingly similar patterns were observed in the visible (bright plumes and blue-gray regions), where reflectivity in the red is anti-correlated with 5-microns thermal radiance. Ortiz et al. (1998, GRL, 103) characterized the latitude and drift rates of the hot spots, including the descent of the Galileo probe at the southern edge of a 5-micron hot spot, as the superposition of equatorial Rossby waves, with phase speeds between 99 - 103m/s, relative to System III. We note that the high 5-micron radiances correlate well but not perfectly with high 8.57-micron radiances. Because the latter are modulated primarily by changes in the upper ammonia (NH3) ice cloud opacity, this correlation implies that changes in the ammonia ice cloud field may be responsible for the variability seen in the 5-m maps. During the NEB fade (2011 - early 2012), however, these otherwise ubiquitous features were absent, an atmospheric state not seen in decades. The ongoing NEB revival indicates nascent 5-m hot spots as early as April 2012, with corresponding visible dark spots. Their continuing growth through July 2012 indicates the possit.le re-establishment of Rossby waves. The South Equatorial Belt (SEB) and NEB revivals began similarly with an instability that developed into a major outbreak, and many similarities in the observed propagation of clear regions.

  1. Combustion properties of micronized coal for high intensity combustion applications

    SciTech Connect

    Freihaut, J.D.; Proscia, W.; Knight, B.; Vranos, A.; Hollick, H.; Wicks, K.

    1989-04-19

    Results are presented of an investigation of combustion related properties of micronized coal feeds (all particles less than 40 microns), mixing characteristics of centrifugally driven burner devices, and aerodynamic characteristics of micronized coal particles related to centrifugal mixing for high intensity combustion applications. Combustion related properties investigated are the evolution of fuel bound nitrogen and coal associated mineral matter during the initial stages of combustion. Parent and beneficiated micronized coal samples, as well as narrow size cut samples from a wide range of coal ranks, were investigated using a multireactor approach. The multireactor approach allowed the experimental separation of different aspects of the fuel nitrogen evolution process, enabling a comprehensive understanding of FBN to be formulated and empirical rate constants to be developed. A specially designed on-line gas analysis system allowed nitrogen balance to be achieved. A combined nitrogen and ash tracer technique allowed the quantitative determination of tar yields during rapid devolatilization. Empirical kinetic rates are developed for the evolution of FBN with tar at low temperatures and the appearance of HCN from tar and char species at high temperatures. A specially designed phase separation system, coupled to separate aerosol and char segregation trains, allowed the possible formation of ash aerosol by rapid devolatilization to be monitored. Compensated thermocouple, hot wire anemometry, and digital imaging techniques are employed to characterize the mixing properties of a centrifugally driven combustor. Analytical and experimental investigations of the fidelity of micronized coal particles to gas stream trajectories in the strong centrifugal fields are performed. Both spherical and nonspherical particle morphologies are considered analytically. 14 refs., 141 figs., 34 tabs.

  2. Ultra-low emittance beam generation using two-color ionization injection in a CO2 laser-driven plasma accelerator

    SciTech Connect

    Schroeder, Carl; Benedetti, Carlo; Bulanov, Stepan; Chen, Min; Esarey, Eric; Geddes, Cameron; Vay, J.; Yu, Lule; Leemans, Wim

    2015-05-21

    Ultra-low emittance (tens of nm) beams can be generated in a plasma accelerator using ionization injection of electrons into a wakefield. An all-optical method of beam generation uses two laser pulses of different colors. A long-wavelength drive laser pulse (with a large ponderomotive force and small peak electric field) is used to excite a large wakefield without fully ionizing a gas, and a short-wavelength injection laser pulse (with a small ponderomotive force and large peak electric field), co-propagating and delayed with respect to the pump laser, to ionize a fraction of the remaining bound electrons at a trapped wake phase, generating an electron beam that is accelerated in the wake. The trapping condition, the ionized electron distribution, and the trapped bunch dynamics are discussed. Expressions for the beam transverse emittance, parallel and orthogonal to the ionization laser polarization, are presented. An example is shown using a 10-micron CO2 laser to drive the wake and a frequency-doubled Ti:Al2O3 laser for ionization injection.

  3. Saturn's Deep Cloud Structure Derived From 5-Micron Spectra

    NASA Astrophysics Data System (ADS)

    Bjoraker, G. L.; Chanover, N. J.; Glenar, D. A.; Hewagama, T.

    2007-12-01

    The CSHELL and SpeX spectrometers on NASA's Infrared Telescope Facility were used to observe Saturn between 4.5 and 5.4 microns on several occasions from 2004-2007 at the same time as Cassini/VIMS and CIRS were mapping the planet. At these wavelengths thermal radiation originates from the deep atmosphere (5 bars) and it is attenuated by two cloud layers considered in equilibrium models to be composed of NH4SH and condensed NH3. In addition, there is a component of sunlight reflected from the upper (NH3) cloud that varies spatially on Saturn. CSHELL can spectrally resolve profiles of absorption lines of ammonia (NH3) and phosphine (PH3) on Saturn at selected wavelengths. These lines are very broad due to collisions with 3 to 5 bars of hydrogen. The Saturn spectrum exhibits numerous strong NH3 and PH3 lines, as well as Fraunhofer lines due to CO in the Sun. SpeX observations cover the entire 5-micron window sampling both thermal emission and reflected sunlight. Image cubes were obtained by stepping the slit across the planet. The best contrast in reconstructed images occurs at 5.05 microns, which coincides with the wavelength where VIMS sees spectacular structure on Saturn. The spatial variation of Saturn's 5-micron spectrum is dominated by the variable opacity of its deep cloud structure. Superimposed on this are smaller variations in the mixing ratios of NH3 and PH3. The abundances of these gases can be retrieved reliably in relatively cloud-free regions between 50 South and 65 South, which are analogous to Jupiter's belts and 5-micron hot spots. Elsewhere, it is more difficult to separate changes in cloud opacity from gas abundances. We use near-simultaneous CIRS observations which sound the ~500-mbar level to provide an upper boundary condition to PH3. The 5-micron spectrum of Saturn's Equatorial Zone (10 South) is significantly different from a region near 60 South. The NH3 and PH3 lines are weaker and narrower in the EQZ, while the Fraunhofer lines are stronger

  4. Two color morphs of the pelagic yellow-bellied sea snake, Pelamis platura, from different locations of Costa Rica: snake venomics, toxicity, and neutralization by antivenom.

    PubMed

    Lomonte, Bruno; Pla, Davinia; Sasa, Mahmood; Tsai, Wan-Chih; Solórzano, Alejandro; Ureña-Díaz, Juan Manuel; Fernández-Montes, María Laura; Mora-Obando, Diana; Sanz, Libia; Gutiérrez, José María; Calvete, Juan J

    2014-05-30

    the treatment of envenomations by this species. Integrative analyses of animal venoms that combine the power of proteomics (venomics) with the characterization of their functional and immunological properties are significantly expanding knowledge on these remarkable bioweapons, both from a basic and a medical perspective. Costa Rica harbors a unique population of the yellow-bellied sea snake, Pelamis platura, that is restricted to a fjord-like gulf (Golfo Dulce). This population differs markedly from oceanic populations found elsewhere along the Pacific coast of this country, by presenting a patternless bright yellow coloration, instead of the typical bicolored or tricolored pattern of this species. It has been suggested that the dominance of this yellow-morph in Golfo Dulce might reflect gene flow restrictions, caused by the oceanographic conditions at this location. The present study demonstrates that the remarkable phenotypic variation between the two color morphs inhabiting Golfo Dulce and Golfo de Papagayo, respectively, is not associated with differences in the expression of venom components, as shown by their conserved RP-HPLC profiles. Proteomic analysis revealed the relatively simple toxin composition of P. platura venom, which contains three predominant types of proteins: three-finger toxins (protein abundance: 49.9%), phospholipases A2 (32.9%), and cysteine-rich secretory proteins (9.1%), together with few minor components. Further, the involvement of these most abundant proteins in the toxic effects of the venom, and their cross-recognition and neutralization by a sea snake antivenom produced against the venom of Enhydrina schistosa, were analyzed. Copyright © 2014 Elsevier B.V. All rights reserved.

  5. The BRCA1 alternative splicing variant Δ14-15 with an in-frame deletion of part of the regulatory serine-containing domain (SCD) impairs the DNA repair capacity in MCF-7 cells.

    PubMed

    Sevcik, Jan; Falk, Martin; Kleiblova, Petra; Lhota, Filip; Stefancikova, Lenka; Janatova, Marketa; Weiterova, Lenka; Lukasova, Emilie; Kozubek, Stanislav; Pohlreich, Petr; Kleibl, Zdenek

    2012-05-01

    The BRCA1 gene codes for a protein involved in the DNA double strand break (DDSB) repair. Alongside the dominant full-length splicing form of BRCA1, numerous endogenously expressed alternative splicing variants of unknown significance have been described in various tissues. Some of them retain the original BRCA1 reading frame but lack several critical BRCA1 structural domains, suggesting an altered function of the resulting protein in the BRCA1-regulated processes. To characterize the effect of the BRCA1Δ14-15 splicing variant (with an in-frame deletion affecting the regulatory serine-containing domain) on the DDSB repair, we constructed the MCF-7 clones stably expressing the analyzed variant with/without a shRNA-mediated downregulation of the endogenous full-length wild-type BRCA1 expression. Our results show that the expression of the BRCA1Δ14-15 variant delays the γ-radiation-induced DDSB repair, alters the kinetics of irradiation-induced foci formation/decomposition and reduces the non-homologous end-joining capacity in MCF-7 cells. Therefore, the BRCA1Δ14-15 is not able to functionally replace the full-length wt BRCA1 in the DDSB repair. Our findings indicate that the endogenously expressed BRCA1 alternative splicing variants may negatively influence genome stability and support the growing evidence of the pathological potential of the sequence variants generated by an altered or misregulated alternative splicing in the process of mammary malignant transformation.

  6. 60 micron luminosity evolution of rich clusters of galaxies

    SciTech Connect

    Kelly, D.M.; Rieke, G.H. )

    1990-10-01

    The average 60-micron flux has been determined for a collection of optically selected galaxy clusters at redshifts ranging from 0.30 to 0.92. The result, 26 mJy per cluster, represents the faintest flux determination known of using the IRAS data base. The flux from this set of clusters has been compared to the 60-micron flux from a sample of nearby galaxy clusters. It is found that the far-infrared luminosity evolution in cluster galaxies can be no more than a factor of 1.7 from z = 0.4 to the present epoch. This upper limit is close to the evolution predicted for simple aging of the stellar populations. Additional processes such as mergers, cannibalism, or enhanced rates of starbursts appear to occur at a low enough level that they have little influence on the far-infrared emission from clusters over this redshift range. 38 refs.

  7. 60 micron luminosity evolution of rich clusters of galaxies

    NASA Technical Reports Server (NTRS)

    Kelly, Douglas M.; Rieke, George H.

    1990-01-01

    The average 60-micron flux has been determined for a collection of optically selected galaxy clusters at redshifts ranging from 0.30 to 0.92. The result, 26 mJy per cluster, represents the faintest flux determination known of using the IRAS data base. The flux from this set of clusters has been compared to the 60-micron flux from a sample of nearby galaxy clusters. It is found that the far-infrared luminosity evolution in cluster galaxies can be no more than a factor of 1.7 from z = 0.4 to the present epoch. This upper limit is close to the evolution predicted for simple aging of the stellar populations. Additional processes such as mergers, cannibalism, or enhanced rates of starbursts appear to occur at a low enough level that they have little influence on the far-infrared emission from clusters over this redshift range.

  8. Radiation Hard 0.13 Micron CMOS Library at IHP

    NASA Astrophysics Data System (ADS)

    Jagdhold, U.

    2013-08-01

    To support space applications we have developed an 0.13 micron CMOS library which should be radiation hard up to 200 krad. The article describes the concept to come to a radiation hard digital circuit and was introduces in 2010 [1]. By introducing new radiation hard design rules we will minimize IC-level leakage and single event latch-up (SEL). To reduce single event upset (SEU) we add two p-MOS transistors to all flip flops. For reliability reasons we use double contacts in all library elements. The additional rules and the library elements are integrated in our Cadence mixed signal design kit, “Virtuoso” IC6.1 [2]. A test chip is produced with our in house 0.13 micron BiCMOS technology, see Ref. [3]. As next step we will doing radiation tests according the european space agency (ESA) specifications, see Ref. [4], [5].

  9. Tunable diode lasers for 3-30 micron infrared operation

    NASA Technical Reports Server (NTRS)

    Linden, K. J.

    1983-01-01

    The tunable diode laser is now widely used in high resolution infrared spectroscopy studies, taking into account laboratory and industrial applications. The present investigation is concerned with advances related to laser performance and reliability. The advances are the result of improvements in materials and device technologies. Reliability data for broad-area Pb(1-x)Sn(x)Se lasers are considered along with performance improvements in stripe-geometry lasers, laser performance at wavelengths above 25 microns, and laser performance at wavelengths below 4 microns. Attention is given to tunable Pb-salt infrared diode lasers, mesa-stripe geometry lasers of Pb(1-x)Sn(x)Se and PbS(1-x)Se(x), and long wavelength diode laser emission observed in both Pb(1-x)Sn(x)Te and Pb(1-x)Sn(x)Se.

  10. Correlation of infrared reflectance ratios at 2.3 microns/1.6 micron and 1.1 micron/1.6 micron with delta O-18 values delineating fossil hydrothermal systems in the Idaho batholith

    NASA Technical Reports Server (NTRS)

    Gillespie, A. R.; Criss, R. E.

    1983-01-01

    Reflectance ratios from laboratory spectra and airborne multispectral images are found to be strongly correlated with delta O-18 values of granite rocks in the Idaho batholith. The correlation is largely a result of interactions between hot water and rock, which lowered the delta O-18 values of the rocks and produced secondary hydrous material. Maps of the ratio of reflectivities at 2.3 and 1.6 microns should delineate fossil hydrothermal systems and provide estimates of alteration intensity. However, hydrous minerals produced during deuteric alteration or weathering cannot be unambiguously distinguished in remotely sensed images from the products of propylitic alteration without the use of narrow-band scanners. The reflectivity at 1.6 micron is strongly correlated with rock density and may be useful in distinguishing rock types in granitic terranes.

  11. Radiation Pressure Measurements on Micron-Size Individual Dust Grains

    NASA Technical Reports Server (NTRS)

    Abbas, M. M.; Craven, P. D.; Spann, J. F.; Witherow, W. K.; West, E. A.; Gallagher, D. L.; Adrian, M. L.; Fishman, G. J.; Tankosic, D.; LeClair, A.

    2003-01-01

    Measurements of electromagnetic radiation pressure have been made on individual silica (SiO2) particles levitated in an electrodynamic balance. These measurements were made by inserting single charged particles of known diameter in the 0.2- to 6.82-micron range and irradiating them from above with laser radiation focused to beam widths of approximately 175- 400 microns at ambient pressures particle due to the radiation force is balanced by the electrostatic force indicated by the compensating dc potential applied to the balance electrodes, providing a direct measure of the radiation force on the levitated particle. Theoretical calculations of the radiation pressure with a least-squares fit to the measured data yield the radiation pressure efficiencies of the particles, and comparisons with Mie scattering theory calculations provide the imaginary part of the refractive index of SiO2 and the corresponding extinction and scattering efficiencies.

  12. Radiation Pressure Measurements on Micron-Size Individual Dust Grains

    NASA Technical Reports Server (NTRS)

    Abbas, M. M.; Craven, P. D.; Spann, J. F.; Witherow, W. K.; West, E. A.; Gallagher, D. L.; Adrian, M. L.; Fishman, G. J.; Tankosic, D.; LeClair, A.

    2003-01-01

    Measurements of electromagnetic radiation pressure have been made on individual silica (SiO2) particles levitated in an electrodynamic balance. These measurements were made by inserting single charged particles of known diameter in the 0.2- to 6.82-micron range and irradiating them from above with laser radiation focused to beam widths of approximately 175- 400 microns at ambient pressures particle due to the radiation force is balanced by the electrostatic force indicated by the compensating dc potential applied to the balance electrodes, providing a direct measure of the radiation force on the levitated particle. Theoretical calculations of the radiation pressure with a least-squares fit to the measured data yield the radiation pressure efficiencies of the particles, and comparisons with Mie scattering theory calculations provide the imaginary part of the refractive index of SiO2 and the corresponding extinction and scattering efficiencies.

  13. Infrared spectrum of Io, 2.8-5.2 microns

    NASA Technical Reports Server (NTRS)

    Cruikshank, D. P.

    1980-01-01

    The reflectance spectrum of Io is presented from 2.8 to 5.2 microns demonstrating the full extent of the broad and deep spectral absorption between 3.5 and 4.8 microns. Laboratory spectra of nitrates and carbonates diluted with sulfur do not satisfactorily reproduce the Io spectrum, but new information based on recently discovered volcanic activity on the satellite lead to consideration of other classes of compounds reported by Fanale et al. (1979). It is concluded that the variability of the supply of condensible SO2 gas to the surface of Io, its removal by sublimination, and the temporal variations in the strength of the SO2 band may provide an index of volcanic activity on Io that can be monitored from the earth.

  14. Photometric variability of Charon at 2.2 microns

    NASA Technical Reports Server (NTRS)

    Bosh, A. S.; Young, L. A.; Elliot, J. L.; Hammel, H. B.; Baron, R. L.

    1992-01-01

    Pluto-Charon images obtained on each of four nights at 2.2, 1.2, and 1.7 microns are presently fitted by a two-source image model in which the position of Charon and the ratio of its signal to that of Pluto are free parameters. At 2.2 microns, Charon is fainter than Pluto by magnitudes which, when combined with Pluto-Charon system photometry, yield apparent magnitudes of 15.01 + or - 0.08 for Charon at 0.06 lightcurve phase and 15.46 + or - 0.05 at lightcurve phase 0.42. In view of these results, Charon is variable in this filter bypass due to geometric albedo changes as a function of longitude.

  15. Luminous infrared galaxies - Sizes at 10-32 microns

    NASA Technical Reports Server (NTRS)

    Wynn-Williams, C. G.; Becklin, E. E.

    1993-01-01

    We have made estimates of the sizes of 19 infrared galaxies drawn from the highest luminosity galaxies detected by the IRAS survey. The techniques we used were to make multiaperture photometric measurements on the ground-based IR Telescope Facility and to compare these flux densities with the much broader beam measurements obtained by the IRAS survey. Our primary result is that most, but not all, of the galaxies in our sample are extended at 10-25 microns, with characteristic radii of a few hundred parsecs. This result directly supports the widely held assumption that the bulk of the infrared luminosity in these galaxies comes from a central starburst region rather than from the whole disk of the galaxy or from a compact nucleus. The compact nature of Arp 220 is confirmed by direct scans with a 2.9 arcsec aperture at 32 microns.

  16. Correlation of infrared reflectance ratios at 2.3 microns/1.6 micron and 1.1 micron/1.6 micron with delta O-18 values delineating fossil hydrothermal systems in the Idaho batholith

    NASA Technical Reports Server (NTRS)

    Gillespie, A. R.; Criss, R. E.

    1983-01-01

    Reflectance ratios from laboratory spectra and airborne multispectral images are found to be strongly correlated with delta O-18 values of granite rocks in the Idaho batholith. The correlation is largely a result of interactions between hot water and rock, which lowered the delta O-18 values of the rocks and produced secondary hydrous material. Maps of the ratio of reflectivities at 2.3 and 1.6 microns should delineate fossil hydrothermal systems and provide estimates of alteration intensity. However, hydrous minerals produced during deuteric alteration or weathering cannot be unambiguously distinguished in remotely sensed images from the products of propylitic alteration without the use of narrow-band scanners. The reflectivity at 1.6 micron is strongly correlated with rock density and may be useful in distinguishing rock types in granitic terranes.

  17. LED pumped micron-scale all-silicon Raman amplifier

    NASA Astrophysics Data System (ADS)

    Datta, Tanmoy; Sen, Mrinal

    2017-10-01

    A micron-scale all-silicon Raman amplifier has been proposed in this paper, exploiting the giant Raman gain of silicon nanocrystal material along with the extreme optical confinement of slotted photonic crystal waveguide. Light Emitting Diode (LED) has been considered here for low-cost optical pumping and the possibility of on-chip integration. At the same time, LED pumping eradicates the temporal impairment of output pulses which is otherwise unavoidable in case of continuous wave laser pumping. An overall gain of 3.22 dB has been achieved for a 400 Gbps input pulse train with a waveguide length of the amplifier which is as small as 4 μm. Moreover, the strong electroluminescence of silicon nanocrystal opens up the possibility of integrating the pump source on the same platform and, hence, expedites the future scope of realizing micron-scale silicon Raman laser without external pump source.

  18. AN ACCURATE DETERMINATION OF THE MICRON + MAGNETIC MOMENT,

    DTIC Science & Technology

    magnetic resonance magnetometer . The ratio of the muon precession frequency to that of the proton in the same magnetic field is determined to be...measurements of the beat note. The magnetic field at which the precession and reference frequencies coincide is measured with reference to a proton nuclear...Using a precession technique, the magnetic moment of the positive micron meson is determined to an accuracy of 0.007%. Muons are brought to rest in

  19. Micro Navigator (MICRON) Phase 2A. Volume 1. Technical Report

    DTIC Science & Technology

    1976-02-01

    AD-A021 526 MICRO NAVIGATOR (MICRON) PHASE 2A. VOLUME I. TECHNICAL REPORT Joseph M. Miller Rockwell International Corporation Prepared for:, Air...DOCUMENT IS BEST QUALITY PRACTICABLE. THE COPY FURNISHED TO DTIC CONTAINED A SIGNIFICANT NUMBER OF PAGES WHICH DO NOT REPRODUCE LEGIBLY. WAFA-TR-7-t MICRO ...Calibration Optilization Mass Unbalance Modulaticn N57A Flights Tests Cost of Ownosh , Micro Electrostatic Gyrc Small Gap Gyco Dift Model Improvement Micro

  20. Development program for 1.93-micron lasers

    NASA Technical Reports Server (NTRS)

    Longeway, P.; Zamerowski, T.; Martinelli, R.; Stolzenberger, R.; Digiuseppe, N.

    1988-01-01

    For the first time lasers operating at 1.93 microns were demonstrated. The lasers were fabricated by Vapor Phase Epitaxial (VPE) growth techniques currently used for the fabrication of high power lasers at 1.3 microns. The structure of these laser diodes consisted of compositionally graded, sulfur-doped InAsP, grown on an InP substrate; a constant-composition n+InAs(0.27)P(0.73) layer, which is the first cladding layer; an In(0.66)Ga(0.34)As layer, which is the active region, and a second InAs(0.27)P(0.73) layer. The devices were oxide-stripe DH lasers (gain-guided only). The best devices had 80 K lasing thresholds in the range of from 80 to 150 mA, and T sub o (below 220 K) in the range of 60 to 90 K. The highest observed temperature of oscillation was 15.5 C. The highest observed power output at 80 K was in the range of 3 to 5 mW. The calculated delta I/delta T was 4.4 A/K. As a part of the materials development, PIN homojunction detectors having the band edge near 1.93 were also fabricated. The best devices (100 micron diameter, mesa structure) exhibited room temperature dark currents in the range of from 20 to 50 nA and had QE at 1.93 microns in the range of 35 to 40 percent. In addition to the device results, the InGaAs-InAsP materials system was extensively investigated and low defect density layers can now be grown allowing for significant device performance improvement.

  1. Optics outreach from 8-12 microns: Wolfe-inspired

    NASA Astrophysics Data System (ADS)

    Nofziger, Michael J.

    2012-10-01

    Using a room-temperature FLIR infrared camera, we have developed an entire outreach program that allows students of all ages the chance to "see" their world from 8-12 microns. It is a world seldom seen by the same person that, ironically, has 12 megapixels of visual "high-def" in his or her shirt pocket. It is Bill Wolfe's world, and in his recognition we are honored to share some of it with you.

  2. Two micron pore size MCP-based image intensifiers

    NASA Astrophysics Data System (ADS)

    Glesener, John; Estrera, Joseph

    2010-02-01

    Image intensifiers (I2) have many advantages as detectors. They offer single photon sensitivity in an imaging format, they're light in weight and analog I2 systems can operate for hours on a single AA battery. Their light output is such as to exploit the peak in color sensitivity of the human eye. Until recent developments in CMOS sensors, they also were one of the highest resolution sensors available. The closest all solid state solution, the Texas Instruments Impactron chip, comes in a 1 megapixel format. Depending on the level of integration, an Impactron based system can consume 20 to 40 watts in a system configuration. In further investing in I2 technology, L-3 EOS determined that increasing I2 resolution merited a high priority. Increased I2 resolution offers the system user two desirable options: 1) increased detection and identification ranges while maintaining field-of-view (FOV) or 2) increasing FOV while maintaining the original system resolution. One of the areas where an investment in resolution is being made is in the microchannel plate (MCP). Incorporation of a 2 micron MCP into an image tube has the potential of increasing the system resolution of currently fielded systems. Both inverting and non-inverting configurations are being evaluated. Inverting tubes are being characterized in night vision goggle (NVG) and sights. The non-inverting 2 micron tube is being characterized for high resolution I2CMOS camera applications. Preliminary measurements show an increase in the MTF over a standard 5 micron pore size, 6 micron pitch plate. Current results will be presented.

  3. Dissolution and pharmacokinetics of a novel micronized aspirin formulation.

    PubMed

    Voelker, M; Hammer, M

    2012-08-01

    Aspirin (acetylsalicylic acid, ASA) has been used as an analgesic, antipyretic and antiinflammatory drug for many years. A new 500 mg aspirin tablet formulation containing micronized active ingredient and an effervescent component has been developed for potential improvement in the onset of action for acute pain treatment. This paper describes the dissolution and the pharmacokinetics of the new formulation in comparison with regular aspirin tablets, aspirin granules and aspirin effervescent tablets. Micronized aspirin tablets dissolve significantly faster over a pH range from 1.2 to 6.8 compared to regular 500 mg aspirin tablets. Plasma concentration time curve comparison to regular 500 mg aspirin tablets showed a substantial improvement in the time to maximum plasma concentrations (T(max)) (ASA 17.5 min vs. 45 min) and an increase in maximum plasma concentration (C(max)) (ASA 13.8 μg/ml vs. 4.4 μg/ml) while the overall extent of exposure (AUC) remains almost unchanged. The data suggest a potential improvement for onset of action in treating acute pain with the new micronized aspirin formulation.

  4. Scanning SQUID susceptometers with sub-micron spatial resolution

    SciTech Connect

    Kirtley, John R. Rosenberg, Aaron J.; Palmstrom, Johanna C.; Holland, Connor M.; Moler, Kathryn A.; Paulius, Lisa; Spanton, Eric M.; Schiessl, Daniel; Jermain, Colin L.; Gibbons, Jonathan; Fung, Y.-K.K.; Gibson, Gerald W.; Huber, Martin E.; Ralph, Daniel C.; Ketchen, Mark B.

    2016-09-15

    Superconducting QUantum Interference Device (SQUID) microscopy has excellent magnetic field sensitivity, but suffers from modest spatial resolution when compared with other scanning probes. This spatial resolution is determined by both the size of the field sensitive area and the spacing between this area and the sample surface. In this paper we describe scanning SQUID susceptometers that achieve sub-micron spatial resolution while retaining a white noise floor flux sensitivity of ≈2μΦ{sub 0}/Hz{sup 1/2}. This high spatial resolution is accomplished by deep sub-micron feature sizes, well shielded pickup loops fabricated using a planarized process, and a deep etch step that minimizes the spacing between the sample surface and the SQUID pickup loop. We describe the design, modeling, fabrication, and testing of these sensors. Although sub-micron spatial resolution has been achieved previously in scanning SQUID sensors, our sensors not only achieve high spatial resolution but also have integrated modulation coils for flux feedback, integrated field coils for susceptibility measurements, and batch processing. They are therefore a generally applicable tool for imaging sample magnetization, currents, and susceptibilities with higher spatial resolution than previous susceptometers.

  5. Soft tissue engineering with micronized-gingival connective tissues.

    PubMed

    Noda, Sawako; Sumita, Yoshinori; Ohba, Seigo; Yamamoto, Hideyuki; Asahina, Izumi

    2017-02-24

    The free gingival graft (FGG) and connective tissue graft (CTG) are currently considered to be the gold standards for keratinized gingival tissue reconstruction and augmentation. However, these procedures have some disadvantages in harvesting large grafts, such as donor-site morbidity as well as insufficient gingival width and thickness at the recipient site post-treatment. To solve these problems, we focused on an alternative strategy using micronized tissue transplantation (micro-graft). In this study, we first investigated whether transplantation of micronized gingival connective tissues (MGCTs) promotes skin wound healing. MGCTs (≤100 µm) were obtained by mincing a small piece (8 mm(3) ) of porcine keratinized gingiva using the RIGENERA system. The MGCTs were then transplanted to a full skin defect (5 mm in diameter) on the dorsal surface of immunodeficient mice after seeding to an atelocollagen matrix. Transplantations of atelocollagen matrixes with and without micronized dermis were employed as experimental controls. The results indicated that MGCTs markedly promote the vascularization and epithelialization of the defect area 14 days after transplantation compared to the experimental controls. After 21 days, complete wound closure with low contraction was obtained only in the MGCT grafts. Tracking analysis of transplanted MGCTs revealed that some mesenchymal cells derived from MGCTs can survive during healing and may function to assist in wound healing. We propose here that micro-grafting with MGCTs represents an alternative strategy for keratinized tissue reconstruction that is characterized by low morbidity and ready availability. © 2017 Wiley Periodicals, Inc.

  6. Radiation Pressure Measurements on Micron Size Individual Dust Grains

    NASA Technical Reports Server (NTRS)

    Abbas, M. M.; Craven, P.D.; Spann, J. F.; Tankosic, D.; Witherow, W. K.; LeClair, A.; West, E.; Sheldon, R.; Gallagher, D. L.; Adrian, M. L.

    2003-01-01

    Measurements of electromagnetic radiation pressure have been made on individual silica (SiO2) particles levitated in an electrodynamic balance. These measurements were made by inserting single charged particles of known diameter in the 0.2 micron to 6.82 micron range and irradiating them from above with laser radiation focused to beam-widths of approx. 175-400 micron, at ambient pressures approx. 10(exp -3) to 10(exp -4) torr. The downward displacement of the particle due to the radiation force is balanced by the electrostatic force indicated by the compensating dc potential applied to the balance electrodes, providing a direct measure of the radiation force on the levitated particle. Theoretical calculations of the radiation pressure with a least-squares fit to the measured data yield the radiation pressure efficiencies of the particles, and comparisons with Mie scattering theory calculations provide the imaginary part of the refractive index of silica and the corresponding extinction and scattering efficiencies.

  7. Effect of humidity on aerosolization of micronized drugs.

    PubMed

    Young, Paul M; Price, Robert; Tobyn, Michael J; Buttrum, Mark; Dey, Fiona

    2003-10-01

    The variation of aerosolization with humidity for three micronized drugs used in the treatment of asthma was evaluated by using in vitro methods. Micronized samples of disodium cromoglycate (DSCG), salbutamol sulphate, and triamcinolone acetonide (TAA) were stored for 12hr at 15, 30, 45, 60, and 75% relative humidity (RH). A suitable "reservoir" dry powder inhaler was loaded and tested by using a twin-stage impinger at each specific humidity. The aerosolization efficiency of all three micronized drugs was affected by variations in humidity. The percentage of the delivered dose and the fine particle fraction of the loaded dose (FPFLD) for both DSCG and salbutamol sulphate decreased with increasing humidity; with the largest decrease in FPFLD occurring between 45% and 60% RH for DSCG and 60% to 75% RH for salbutamol sulphate. These observations suggest that the adhesion properties for both DSCG and salbutamol sulphate, which govern the aerosolization efficiency, are predominately influenced by capillary interactions. In contrast, the FPFLD for TAA significantly increased as the humidity increased over the range 15% to 75% RH, suggesting that triboelectric forces predominate particle-particle interactions. These variations in drug particulate behavior highlight the importance of an individual formulation approach when developing dry powder inhalation systems.

  8. Radiation Pressure Measurements on Micron Size Individual Dust Grains

    NASA Technical Reports Server (NTRS)

    Abbas, M. M.; Craven, P.D.; Spann, J. F.; Tankosic, D.; Witherow, W. K.; LeClair, A.; West, E.; Sheldon, R.; Gallagher, D. L.; Adrian, M. L.

    2003-01-01

    Measurements of electromagnetic radiation pressure have been made on individual silica (SiO2) particles levitated in an electrodynamic balance. These measurements were made by inserting single charged particles of known diameter in the 0.2 micron to 6.82 micron range and irradiating them from above with laser radiation focused to beam-widths of approx. 175-400 micron, at ambient pressures approx. 10(exp -3) to 10(exp -4) torr. The downward displacement of the particle due to the radiation force is balanced by the electrostatic force indicated by the compensating dc potential applied to the balance electrodes, providing a direct measure of the radiation force on the levitated particle. Theoretical calculations of the radiation pressure with a least-squares fit to the measured data yield the radiation pressure efficiencies of the particles, and comparisons with Mie scattering theory calculations provide the imaginary part of the refractive index of silica and the corresponding extinction and scattering efficiencies.

  9. Scanning SQUID susceptometers with sub-micron spatial resolution

    NASA Astrophysics Data System (ADS)

    Kirtley, John R.; Paulius, Lisa; Rosenberg, Aaron J.; Palmstrom, Johanna C.; Holland, Connor M.; Spanton, Eric M.; Schiessl, Daniel; Jermain, Colin L.; Gibbons, Jonathan; Fung, Y.-K.-K.; Huber, Martin E.; Ralph, Daniel C.; Ketchen, Mark B.; Gibson, Gerald W.; Moler, Kathryn A.

    2016-09-01

    Superconducting QUantum Interference Device (SQUID) microscopy has excellent magnetic field sensitivity, but suffers from modest spatial resolution when compared with other scanning probes. This spatial resolution is determined by both the size of the field sensitive area and the spacing between this area and the sample surface. In this paper we describe scanning SQUID susceptometers that achieve sub-micron spatial resolution while retaining a white noise floor flux sensitivity of ≈2μΦ0/Hz1/2. This high spatial resolution is accomplished by deep sub-micron feature sizes, well shielded pickup loops fabricated using a planarized process, and a deep etch step that minimizes the spacing between the sample surface and the SQUID pickup loop. We describe the design, modeling, fabrication, and testing of these sensors. Although sub-micron spatial resolution has been achieved previously in scanning SQUID sensors, our sensors not only achieve high spatial resolution but also have integrated modulation coils for flux feedback, integrated field coils for susceptibility measurements, and batch processing. They are therefore a generally applicable tool for imaging sample magnetization, currents, and susceptibilities with higher spatial resolution than previous susceptometers.

  10. An optical trapped nanohand for manipulating micron-sized particles

    NASA Astrophysics Data System (ADS)

    Gibson, Graham; Barron, Louise; Beck, Fiona; Whyte, Graeme; Padgett, Miles

    2006-08-01

    Optical tweezers use the electric-field gradient-force associated with tightly focused laser beams to trap micron-sized objects at the beam focus. Over the last few years optical tweezers have been revolutionized by the addition of spatial light modulators to split the laser beam into many traps that can be individually controlled; a technique called holographic optical tweezers. However, the reliance of optical tweezers on the gradient-force largely restricts their application to transparent objects that are not unduly sensitive to the effects of the laser light. Consequently, the manipulation of metallic particles or sensitive biomaterials can be problematic. In this work we use a holographic tweezers to position multiple silica beads acting as an optical gripper to lift, rotate and move micron-sized objects that otherwise do not lend themselves to tweezers control. We illustrate the use of the optical gripper under real-time joystick control to manipulate micron-sized metallic particles with nano-scale precision.

  11. Bench-Scale Testing of the Micronized Magnetite Process

    SciTech Connect

    Edward R. Torak; Peter J. Suardini

    1997-11-01

    A recent emphasis of the Department of Energy's (DOE's), Coal Preparation Program has been the development of high-efficiency technologies that offer near-term, low-cost improvements in the ability of coal preparation plants to address problems associated with coal fines. In 1992, three cost-shared contracts were awarded to industry, under the first High-Efficiency Preparation (HEP I) solicitation. All three projects involved bench-scale testing of various emerging technologies, at the Federal Energy Technology Center*s (FETC*s), Process Research Facility (PRF). The first HEP I project, completed in mid-1993, was conducted by Process Technology, Inc., with the objective of developing a computerized, on-line system for monitoring and controlling the operation of a column flotation circuit. The second HEP I project, completed in mid-1994, was conducted by a team led by Virginia Polytechnic Institute to test the Mozely Multi-Gravity Separator in combination with the Microcel Flotation Column, for improved removal of mineral matter and pyritic sulfur from fine coal. The last HEP I project, of which the findings are contained in this report, was conducted by Custom Coals Corporation to evaluate and advance a micronized-magnetite-based, fine-coal cycloning technology. The micronized-magnetite coal cleaning technology, also know as the Micro-Mag process, is based on widely used conventional dense-medium cyclone applications, in that it utilizes a finely ground magnetite/water suspension as a separating medium for cleaning fine coal, by density, in a cyclone. However, the micronized-magnetite cleaning technology differs from conventional systems in several ways: ! It utilizes significantly finer magnetite (about 5 to 10 micron mean particle size), as compared to normal mean particle sizes of 20 microns. ! It can effectively beneficiate coal particles down to 500M in size, as compared to the most advanced, existing conventional systems that are limited to a particle bottom

  12. 2-45 Micron Infrared Spectroscopy of Carbon-Rich Proto-Planetary Nebulae

    NASA Technical Reports Server (NTRS)

    Hrivnak, Bruce J.; Volk, Kevin; Kwok, Sun

    2000-01-01

    Infrared Space Observatory (ISO) 2-45 micron observations of seven proto-planetary nebulae (PPNs) and two other carbon-rich objects are presented. The unidentified emission features at 21 and 30 microns are detected in six sources, including four new detections of the 30 micron feature. This previously unresolved 30 micron feature is now resolved and found to consist of a broad feature peaking at 27.2 microns (the '30 micron' feature) and a narrower feature at 25.5 microns (the '26 micron' feature). This new 26 micron feature is detected in eight sources and is particularly strong in IRAS Z02229 + 6208 and 16594-4656. The unidentified infrared (UIR) emission features at 3.3, 6.2, 7.7, and 11.3 microns which are commonly observed in planetary nebulae and H II regions, are also seen in these PPNs. However, their strengths relative to the continuum plateaus at 8 and 12 microns are weaker than in planetary nebulae. The 6.9 micron feature, seen almost exclusively in PPNs, is strong. New millimeter CO and HCN observations were made; they support the carbon-rich nature of the objects and yield the expansion velocities of the gaseous envelopes. The spectral energy distributions of these PPNs were fitted with a radiative-transfer model, taking into account the emission features at 21, 26, and 30 microns. A significant fraction of the total energy output is emitted in these features: as high as 20% in the 30 micron feature and 8% in the 21 micron feature. The fact that so much energy is carried in these features suggests that the material responsible for these features must be made of abundant elements and most likely involves carbon. SiS, appears to be ruled out as the emitter of the 21 micron feature due to the absence of a predicted companion feature.

  13. 2-45 Micron Infrared Spectroscopy of Carbon-Rich Proto-Planetary Nebulae

    NASA Technical Reports Server (NTRS)

    Hrivnak, Bruce J.; Volk, Kevin; Kwok, Sun

    2000-01-01

    Infrared Space Observatory (ISO) 2-45 micron observations of seven proto-planetary nebulae (PPNs) and two other carbon-rich objects are presented. The unidentified emission features at 21 and 30 microns are detected in six sources, including four new detections of the 30 micron feature. This previously unresolved 30 micron feature is now resolved and found to consist of a broad feature peaking at 27.2 microns (the '30 micron' feature) and a narrower feature at 25.5 microns (the '26 micron' feature). This new 26 micron feature is detected in eight sources and is particularly strong in IRAS Z02229 + 6208 and 16594-4656. The unidentified infrared (UIR) emission features at 3.3, 6.2, 7.7, and 11.3 microns which are commonly observed in planetary nebulae and H II regions, are also seen in these PPNs. However, their strengths relative to the continuum plateaus at 8 and 12 microns are weaker than in planetary nebulae. The 6.9 micron feature, seen almost exclusively in PPNs, is strong. New millimeter CO and HCN observations were made; they support the carbon-rich nature of the objects and yield the expansion velocities of the gaseous envelopes. The spectral energy distributions of these PPNs were fitted with a radiative-transfer model, taking into account the emission features at 21, 26, and 30 microns. A significant fraction of the total energy output is emitted in these features: as high as 20% in the 30 micron feature and 8% in the 21 micron feature. The fact that so much energy is carried in these features suggests that the material responsible for these features must be made of abundant elements and most likely involves carbon. SiS, appears to be ruled out as the emitter of the 21 micron feature due to the absence of a predicted companion feature.

  14. Photon Counting Detectors for the 1.0 - 2.0 Micron Wavelength Range

    NASA Technical Reports Server (NTRS)

    Krainak, Michael A.

    2004-01-01

    We describe results on the development of greater than 200 micron diameter, single-element photon-counting detectors for the 1-2 micron wavelength range. The technical goals include quantum efficiency in the range 10-70%; detector diameter greater than 200 microns; dark count rate below 100 kilo counts-per-second (cps), and maximum count rate above 10 Mcps.

  15. The micron stroke hypothesis of Alzheimer's disease and dementia.

    PubMed

    Orehek, Allen J

    2012-05-01

    Alzheimer's disease as currently described in the medical literature is often more a description of dementia rather than a specific disease. In over a century of scientific work there has been no proven theory as to the precise pathogenesis of Alzheimer's disease and dementia. As there is no efficient treatment for patients with Alzheimer's disease, prevention or attenuation of the disease is of substantial value. An intricate collection of hypotheses, studies, research, and experience has made it complicated for one to completely understand this disease. The purpose of this hypothesis is to illustrate new concepts and work to link those concepts to the present understanding of an obscure disease. The search for a single unifying hypothesis on the etiology of Alzheimer's disease has been elusive. Many hypotheses associated to Alzheimer's disease have not survived their testing to become theory. Suggested here is that the elusive nature of etiology of dementia is not from one cause, but rather the causes are numerous. Medical terminology used freely for decades is rarely evaluated in the light of a new hypothesis. At the foundation of this work is the suggestion of a new medical term: Micron Strokes. The Micron Stroke Hypothesis of Alzheimer's Disease and Dementia include primary and secondary factors. The primary factors can be briefly described as baseline brain tissue, atrial fibrillation, hypercoaguable state, LDL, carotid artery stenosis, tobacco exposure, hypertension diabetes mellitus, and the presence of systemic inflammation. Dozens of secondary factors contribute to the development of dementia. Most dementia is caused by nine primary categories of factors as they interact to cause micron strokes to the brain. Copyright © 2012 Elsevier Ltd. All rights reserved.

  16. A High Energy 2-microns Laser for Multiple Lidar Applications

    NASA Technical Reports Server (NTRS)

    Yu, Jirong; Singh, Upendra N.; Barnes, James C.; Barnes, Norman P.; Petros, Mulugeta

    2000-01-01

    Solid-state 2-microns laser has been receiving considerable interest because of its eye-safe property and efficient diode pump operation, It has potential for multiple lidar applications to detect water vapor. carbon dioxide and winds. In this paper, we describe a 2-microns double pulsed Ho:Tm:YLF laser and end-pumped amplifier system. A comprehensive theoretical model has been developed to aid the design and optimization of the laser performance. In a single Q-switched pulse operation the residual energy stored in the Tm atoms will be wasted. However, in a double pulses operation mode, the residual energy stored in the Tm atoms will repopulate the Ho atoms that were depleted by the extraction of the first Q-switched pulse. Thus. the Tin sensitized Ho:YLF laser provides a unique advantage in applications that require double pulse operation, such as Differential Absorption Lidar (DIAL). A total output energy of 146 mJ per pulse pair under Q-switch operation is achieved with as high as 4.8% optical to optical efficiency. Compared to a single pulse laser, 70% higher laser efficiency is realized. To obtain high energy while maintaining the high beam quality, a master-oscillator-power-amplifier 2-microns system is designed. We developed an end-pumped Ho:Tm:YLF disk amplifier. This amplifier uses two diode arrays as pump source. A non-imaging lens duct is used to couple the radiation from the laser diode arrays to the laser disk. Preliminary result shows that the efficiency of this laser can be as high as 3%, a factor of three increases over side-pump configuration. This high energy, highly efficient and high beam quality laser is a promising candidate for use in an efficient, multiple lidar applications.

  17. A High Energy 2-microns Laser for Multiple Lidar Applications

    NASA Technical Reports Server (NTRS)

    Yu, Jirong; Singh, Upendra N.; Barnes, James C.; Barnes, Norman P.; Petros, Mulugeta

    2000-01-01

    Solid-state 2-microns laser has been receiving considerable interest because of its eye-safe property and efficient diode pump operation, It has potential for multiple lidar applications to detect water vapor. carbon dioxide and winds. In this paper, we describe a 2-microns double pulsed Ho:Tm:YLF laser and end-pumped amplifier system. A comprehensive theoretical model has been developed to aid the design and optimization of the laser performance. In a single Q-switched pulse operation the residual energy stored in the Tm atoms will be wasted. However, in a double pulses operation mode, the residual energy stored in the Tm atoms will repopulate the Ho atoms that were depleted by the extraction of the first Q-switched pulse. Thus. the Tin sensitized Ho:YLF laser provides a unique advantage in applications that require double pulse operation, such as Differential Absorption Lidar (DIAL). A total output energy of 146 mJ per pulse pair under Q-switch operation is achieved with as high as 4.8% optical to optical efficiency. Compared to a single pulse laser, 70% higher laser efficiency is realized. To obtain high energy while maintaining the high beam quality, a master-oscillator-power-amplifier 2-microns system is designed. We developed an end-pumped Ho:Tm:YLF disk amplifier. This amplifier uses two diode arrays as pump source. A non-imaging lens duct is used to couple the radiation from the laser diode arrays to the laser disk. Preliminary result shows that the efficiency of this laser can be as high as 3%, a factor of three increases over side-pump configuration. This high energy, highly efficient and high beam quality laser is a promising candidate for use in an efficient, multiple lidar applications.

  18. Advanced composite applications for sub-micron biologically derived microstructures

    NASA Technical Reports Server (NTRS)

    Schnur, J. M.; Price, R. R.; Schoen, P. E.; Bonanventura, Joseph; Kirkpatrick, Douglas

    1991-01-01

    A major thrust of advanced material development is in the area of self-assembled ultra-fine particulate based composites (micro-composites). The application of biologically derived, self-assembled microstructures to form advanced composite materials is discussed. Hollow 0.5 micron diameter cylindrical shaped microcylinders self-assemble from diacetylenic lipids. These microstructures have a multiplicity of potential applications in the material sciences. Exploratory development is proceeding in application areas such as controlled release for drug delivery, wound repair, and biofouling as well as composites for electronic and magnetic applications, and high power microwave cathodes.

  19. Micron-scale lens array having diffracting structures

    DOEpatents

    Goldberg, Kenneth A

    2013-10-29

    A novel micron-scale lens, a microlens, is engineered to concentrate light efficiently onto an area of interest, such as a small, light-sensitive detector element in an integrated electronic device. Existing microlens designs imitate the form of large-scale lenses and are less effective at small sizes. The microlenses described herein have been designed to accommodate diffraction effects, which dominate the behavior of light at small length scales. Thus a new class of light-concentrating optical elements with much higher relative performance has been created. Furthermore, the new designs are much easier to fabricate than previous designs.

  20. The (C II) 158 micron line mapping of spiral galaxies

    NASA Technical Reports Server (NTRS)

    Stacey, Gordon J.; Geis, N.; Genzel, Reinhard; Jackson, J. M.; Poglitsch, Albrecht; Townes, Charles H.

    1990-01-01

    Large scale maps of the face of spiral galaxies M51, M83, and NGC 6946 in the 158 micron (C II) fine structure line. The maps are obtained from the Far-infrared Imaging Fabry-Perot Interferometer (FIFI) during its first series of flights on board the Kuiper Airborne Observatory. The (C II) line emission is ubiquitous and easily traced over the mapped regions of each of the galaxies. The (C II) maps are compared with those obtained with similar sized beams in the CO line. The data available from these maps is interpreted.

  1. Spectrophotometry at 10 microns of T Tauri stars

    NASA Technical Reports Server (NTRS)

    Cohen, M.; Witteborn, F. C.

    1985-01-01

    New 8-13 micron spectra of 32 T Tau, or related young, stars are presented. Silicate emission features are commonly seen. Absorptions occur less frequently but also match the properties of silicate materials. The shape of the emission feature suggests that a more crystalline grain is responsible in the T Tau stars than those of the Trapezium region. The evolution of the silicate component of the circumstellar shell around T Tau stars, and its dependence upon stellar wind activity, visual linear polarization, and extinction are investigated. Several correlations suggest that the shells are likely to be flattened, disklike structures rather than spherical.

  2. Quantitative spectroscopy of micron-thick liquid films

    NASA Astrophysics Data System (ADS)

    Patel, C. K. N.; Tam, A. C.

    1980-01-01

    We demonstrate a new sensitive spectroscopic technique for highly transparent micron-thick liquid films sandwiched between transparent substrates. A pulsed dye laser irradiates the film, producing (via the optoacoustic effect) a transient ultrasonic wave which is launched into the substrate and detected by a piezoelectric transducer bonded to the substrate. This technique is illustrated by measuring the absorption lines of films of aqueous solutions of rare-earth ion, and absorptions ˜10-5 can be detected presently. This technique should open up new opportunities to study the spectra of chemi- or physi-adsorbed species on surfaces.

  3. Fabrication of Micron Scale Retroreflectors for Novel Biosensors

    NASA Astrophysics Data System (ADS)

    Sherlock, Tim

    Many bioanalytical and diagnostic methods detect the presence of secondary labels, such as colored particles, fluorescent molecules, nanoparticles, and enzyme reaction product, when they accumulate in the presence of the target biomolecules (i.e., bacteria, viruses, etc.) at predetermined locations. In this dissertation, we describe the development of a new class of labels consisting of micro-fabricated retroreflectors that are easy to image, compatible with machine vision automation, and can be detected in solution or within microfluidic channels. The retroreflecting structures are designed to return incident light directly back to its source over a large range of angles, making them extremely detectable using low cost, low numerical aperture objectives, as is evidenced by their common use as lane markers and in safety signs. This work describes two different biosensing systems using these labels. In the first, retroreflectors are fabricated at fixed locations at the base of microfluidic channels and their brightness is attenuated by the biologically-driven accumulation of magnetic particles, thus forming a readout strategy that well-suited for automation and multiplexing. The work demonstrates that single, micron-scale magnetic beads can be rapidly detected over very large areas (square millimeters). The second approach uses suspended corner cube retroreflectors, five microns on a side, as ultra bright labels that are bound to magnetic sample preparation beads in the presence of an analyte. The magnetic particles can then be moved to an imaging site within the sample where the cubes are readily detected. The fabrication of these micron-scale retroreflectors required the development of new lithography, thin film disposition, and reactive ion etching tools and the integration of chip-based structures with microfluidic systems. The dissertation also describes the experimental validation of a Fourier optics model that accounts for diffraction inherent to the micron

  4. A Two Micron Coherent Differential Absorption Lidar Development

    NASA Technical Reports Server (NTRS)

    Yu, Jirong; Petros, Mulugeta; Chen, Songsheng; Bai, Yingxin; Petzar, Paul J.; Trieu, Bo C.; Koch, Grady J.; Beyon, Jeffrey Y.; VanValkenburg, Randal L.; Kavaya, Michael J.; Singh, Upendra N.

    2010-01-01

    A pulsed, 2-micron coherent Differential Absorption Lidar (DIAL)/Integrated Path Differential Absorption (IPDA) transceiver, developed under the Laser Risk Reduction Program (LRRP) at NASA, is integrated into a fully functional lidar instrument. This instrument measures atmospheric CO2 profiles (by DIAL) from a ground platform. It allows the investigators to pursue subsequent in science-driven deployments, and provides a unique tool for Active Sensing of CO2 Emissions over Night, Days, and Seasons (ASCENDS) validation that was strongly advocated in the recent ASCENDS Workshop. Keywords: Differential Absorption Lidar, Near Infrared Laser,

  5. The brightest high-latitude 12-micron IRAS sources

    NASA Technical Reports Server (NTRS)

    Hacking, P.; Beichman, C.; Chester, T.; Neugebauer, G.; Emerson, J.

    1985-01-01

    The Infrared Astronomical Satellite (IRAS) Point Source catalog was searched for sources brighter than 28 Jy (0 mag) at 12 microns with absolute galactic latitude greater than 30 deg excluding the Large Magellanic Cloud. The search resulted in 269 sources, two of which are the galaxies NGC 1068 and M82. The remaining 267 sources are identified with, or have infrared color indices consistent with late-type stars some of which show evidence of circumstellar dust shells. Seven sources are previously uncataloged stars. K and M stars without circumstellar dust shells, M stars with circumstellar dust shells, and carbon stars occupy well-defined regions of infrared color-color diagrams.

  6. Variations in the 3 micron spectrum across the Orion Bar: polycyclic aromatic hydrocarbons and related molecules

    NASA Technical Reports Server (NTRS)

    Sloan, G. C.; Bregman, J. D.; Geballe, T. R.; Allamandola, L. J.; Woodward, C. E.

    1997-01-01

    Long-slit spectra across the Orion Bar reveal significant differences in the spatial behavior of the components of the 3 microns polycyclic aromatic hydrocarbon (PAH) spectrum. The strong PAH band at 3.29 microns generally decreases exponentially with distance from the ionization front into the molecular cloud (scale height approximately 12"), although excesses appear approximately 10" and 20" behind the ionization front, close to layers of H2 and CO emission, respectively. The 3.40 microns PAH feature separates into two components with very different spatial distributions. The main component (at 3.395 microns), along with the 3.51 microns band and the PAH plateau (3.3-3.6 microns), shows excess emission approximately 10" and approximately 20" behind the ionization front, stronger than the excesses in the 3.29 microns band. The extra component of the 3.40 microns band, which peaks at approximately 3.405 microns, has a spatial distribution very similar to the H2 emission. Aromatic C-H stretches in PAHs most likely produce the 3.29 microns feature. Aliphatic C-H stretches in either attached methyl side-groups or superhydrogenated PAHs, or perhaps both, could produce the complicated spectral and spatial structure at 3.40 microns.

  7. IRAS low-resolution spectral observations of the 10 and 18 micron silicate emission feature

    NASA Technical Reports Server (NTRS)

    Simpson, Janet P.

    1991-01-01

    The IRAS LRS Atlas contains 8-23 micron spectra of 1816 stars with silicate emission features. In order to study the shapes of the silicate dust features, the 117 stars whose emission features appear to be optically thin and which have the best signal-to-noise ratio at the longest wavelengths were analyzed. Simple spherical dust shell models were calculated in both the optically thin and the slightly optical thick approximations. From the comparison of the predicted spectra of the dust and stellar continuum, the emissivity function Kappa(lambda) was derived. In the different stars, the shape of the 10-micron feature is either narrow or broad and it is peaked either at about 9.7 microns or at 10 microns. Either particle size effects (particles above 0.75 microns in radius) or optical depth effects (central optical depth about 1) could broaden the 10-micron feature. Chemical composition differences no doubt are also important, particularly as regards the position of the peak of the 10-micron feature. The stars with the peak at 10 microns are more closely confined to the Galactic plane than the stars with the peak at 9.7 microns. The shape of the 18-micron feature is essentially the same in all stars, and can be used to extend the interstellar extinction curve past 13 to 22 microns.

  8. Comparative investigations of the effects of the neodymium:YAG laser at 1. 06 microns and 1. 32 microns on tissue

    SciTech Connect

    Frank, F.; Beck, O.J.; Hessel, S.; Keiditsch, E.

    1987-01-01

    The beneficial deep homogeneous coagulation of neodymium (Nd):YAG laser radiation at 1.06 microns owing to low absorption and high scattering in tissue has been documented widely. For another Nd:YAG laser wavelength at 1.32 microns the absorption coefficient of water and saline is approximately ten times higher than at 1.06 microns. This results in more efficient energy conversion into heat in tissue at 1.32 microns. The extinction coefficient in blood at 1.32 microns is only one-third of that at 1.06 microns. We would expect this to result in less heat dissipation by blood and deeper penetration in tissue at 1.32 microns. Nevertheless, at this wavelength scattering also contributes to an effective, uniform distribution of the laser light in the tissue. Animal experiments have been done to examine the effect of wavelength, irradiation time, and beam geometry on tissue damage and to assess its possible clinical uses. The results imply that the 1.32 microns wavelength will produce further indications for the use of the Nd:YAG laser in surgery.

  9. Chip-on-flex with 5-micron features

    NASA Astrophysics Data System (ADS)

    Salmon, Peter C.

    2003-01-01

    A new module packaging method is proposed for electronic systems comprising a motherboard and integrated circuit (IC) chips. Pitches of 10 microns for conductive traces, and 100 microns for bonding pads are achievable. The enabling technology is glass panel manufacture, using equipment and techniques similar to those employed for fabricating liquid crystal display (LCD) panels. Flexible circuits are produced on a glass carrier using a release layer, and the carrier is removed after most of the processing is complete. IC chips are stud bumped and flip chip bonded to wells filled with solder, provided on the flexible circuit. The fabrication density achievable with wafer level packaging (WLP) using silicon wafers is substantially more than is needed for module packaging, as described herein. It is possible to provide WLP performance on glass at a much lower cost. The conductor features on glass are fine enough for the most demanding packaging and assembly techniques. The lowered cost of glass applies to the interconnection circuit plus assembly, test and rework. A test method called Tester-On-Board (TOB) is proposed, employing special-purpose test chips that are directly mounted in the system and mimic the capabilities of external testers. Methods for hermetic sealing, electromagnetic screening, and high-density off-board connections are also proposed.

  10. Tunable UV and compact 2- to 12-micron laser development

    NASA Astrophysics Data System (ADS)

    Swim, Cynthia R.; Fox, Jay A.

    1998-07-01

    The Edgewood Research, Development, and Engineering Center (ERDEC) within the Chemical and Biological Defense Command (CBDCOM) is the Army's principal R&D center for chemical and biological defense technology, engineering, and service. ERDEC has been developing tunable 9 - 11 micron CO2 lidar systems for remote sensing of chemical agents for many years. However, due to the extended range requirements for conventional missions such as fixed site defense and reconnaissance, these systems are relatively large. Smaller, even handheld, standoff detection lidar systems would be useful for the individual warfighter or for decontamination efforts, as well as for numerous environmental monitoring applications. Lidar modeling calculations have been performed for such a system at the Night Vision and Electronic Sensors Directorate, (NVESD) the Army's lead laboratory for low energy lasers. The modeling indicates that fewer than 5 mJ of solid-state laser pulse energy would achieve the required detection sensitivity criteria for standoff chemical agent detection at ranges of several kilometers. This result coupled with recent advances in solid-state laser and frequency conversion technologies allow for extremely compact, tunable lasers and lidars to be produced which are suitable for a handheld standoff detection device. ERDEC has therefore begun an effort in development of compact 2 - 12 micron lasers and lidars. Three different approaches are being investigated and will be described. A review of completed efforts in tunable UV laser source development for remote sensing of biological agents via laser induced fluorescence (LIF) will also be presented.

  11. Chemically generated convective transport of micron sized particles

    NASA Astrophysics Data System (ADS)

    Shklyaev, Oleg; Das, Sambeeta; Altemose, Alicia; Shum, Henry; Balazs, Anna; Sen, Ayusman

    2015-11-01

    A variety of chemical and biological applications require manipulation of micron sized objects like cells, viruses, and large molecules. Increasing the size of particles up to a micron reduces performance of techniques based on diffusive transport. Directional transport of cargo toward detecting elements reduces the delivery time and improves performance of sensing devices. We demonstrate how chemical reactions can be used to organize fluid flows carrying particles toward the assigned destinations. Convection is driven by density variations caused by a chemical reaction occurring at a catalyst or enzyme-covered target site. If the reaction causes a reduction in fluid density, as in the case of catalytic decomposition of hydrogen peroxide, then fluid and suspended cargo is drawn toward the target along the bottom surface. The intensity of the fluid flow and the time of cargo delivery are controlled by the amount of reagent in the system. After the reagent has been consumed, the fluid pump stops and particles are found aggregated on and around the enzyme-coated patch. The pumps are reusable, being reactivated upon injection of additional reagent. The developed technique can be implemented in lab-on-a-chip devices for transportation of micro-scale object immersed in solution.

  12. Intracellular micro-rheology probed by micron-sized wires.

    PubMed

    Chevry, Loudjy; Colin, Rémy; Abou, Bérengère; Berret, Jean-François

    2013-09-01

    In the last decade, rapid advances have been made in the field of micro-rheology of cells and tissues. Given the complexity of living systems, there is a need for the development of new types of nano- and micron-sized probes, and in particular of probes with controlled interactions with the surrounding medium. In the present paper, we evaluate the use of micron-sized wires as potential probes of the mechanical properties of cells. The wire-based micro-rheology technique is applied to living cells such as murine fibroblasts and canine kidney epithelial cells. The mean-squared angular displacement of wires associated to their rotational dynamics is obtained as a function of the time using optical microscopy and image processing. It reveals a Brownian-like diffusive regime of the form Δψ(2)(t,L) ≈ t/L(3), where L denotes the wire length. This scaling suggests that an effective viscosity of the intracellular medium can be determined, and that in the range 1-10 μm it does not depend on the length scale over which it is measured.

  13. Influence of micronization on improving phytoestrogenic effects of wheat bran.

    PubMed

    Yang, Jinghui; Wu, Qinyan; Chen, Hongzhou; Zhuang, Yiqing

    2015-01-01

    The influence of micronization on improving the phytoestrogenic effects of wheat bran was studied. Wheat bran samples were prepared by ball milling, and an animal experiment was carried out by feeding 8-month-old female rats wheat bran. The effect of wheat bran samples on serum estradiol (E2) and progesterone (P) levels of female 8-month-old rats was investigated. The wheat bran with a median diameter of 392.1 μm was micronized to 91.1 and 9.7 μm in median diameter by dry milling and wet milling for 5 hours, respectively. Microscopic observation and X-ray diffraction revealed more potential damage from wet milling than dry milling on the crystal structure of wheat bran granules. Almost all particles were dissolved and there was no obvious crystal peak in the 5-hour wet-milled wheat bran. The serum E2 and P levels of the 8-month-old rats fed wet-milled bran were increased significantly, 2.2 times higher than that of the same aged control group. The experimental results indicated that wet milling could destroy the crystal structure of wheat bran granules and improve the phytoestrogenic effects of wheat bran.

  14. Limestone weathering rates accelerated by micron-scale grain detachment

    NASA Astrophysics Data System (ADS)

    Emmanuel, S.; Levenson, Y.

    2014-12-01

    The weathering rates of carbonate rocks is often thought to be controlled by chemical dissolution, although some studies have suggested that mechanical erosion could also play an important role. Quantifying the rates of the different processes has proved challenging due to the high degree of variability encountered in both field and lab settings. To determine the rates and mechanisms controlling long-term limestone weathering, we analyse a lidar scan of the Western Wall, a Roman period edifice located in Jerusalem. Weathering rates in fine-grained micritic limestone blocks are up to 2 orders of magnitude higher than the average rates estimated for coarse-grained limestone blocks at the same site. In addition, in experiments that use atomic force microscopy to image dissolving micritic limestone, we show that these higher reaction rates could be due to rapid dissolution along micron-scale grain boundaries, followed by mechanical detachment of tiny particles from the surface. Our analysis indicates that micron-scale grain detachment, rather than pure chemical dissolution, could be the dominant erosional mode for fine-grained rocks in many carbonate terrains.

  15. Micron: an Actively Stabilized Handheld Tool for Microsurgery

    PubMed Central

    MacLachlan, Robert A.; Becker, Brian C.; Tabarés, Jaime Cuevas; Podnar, Gregg W.; Lobes, Louis A.; Riviere, Cameron N.

    2011-01-01

    We describe the design and performance of a hand-held actively stabilized tool to increase accuracy in micro-surgery or other precision manipulation. It removes involuntary motion such as tremor by actuating the tip to counteract the effect of the undesired handle motion. The key components are a three-degree-of-freedom piezoelectric manipulator that has 400 μm range of motion, 1 N force capability, and bandwidth over 100 Hz, and an optical position measurement subsystem that acquires the tool pose with 4 μm resolution at 2000 samples/s. A control system using these components attenuates hand motion by at least 15 dB (a fivefold reduction). By considering the effect of the frequency response of Micron on the human visual feedback loop, we have developed a filter that reduces unintentional motion, yet preserves intuitive eye-hand coordination. We evaluated the effectiveness of Micron by measuring the accuracy of the human/machine system in three simple manipulation tasks. Handheld testing by three eye surgeons and three non-surgeons showed a reduction in position error of between 32% and 52%, depending on the error metric. PMID:23028266

  16. VTT's micron-scale silicon rib+strip waveguide platform

    NASA Astrophysics Data System (ADS)

    Aalto, Timo; Harjanne, Mikko; Cherchi, Matteo

    2016-05-01

    Silicon rib waveguides enable single-mode (SM) operation even with the combination of multi-micron core dimensions and high refractive index contrast. In such large waveguides the optical mode field is almost completely confined inside the Si core, which leads to small propagation losses and small polarization dependency. The unique SM condition of the rib waveguide also enables the use of an ultra-wide wavelength range, for example from 1.2 to <1.7 μm, without sacrificing either SM operation or low propagation loss. This makes micron-scale Si waveguides particularly well-suited for spectroscopy and extensive wavelength division multiplexing. However, rib waveguides require large bending radii, which lead to large circuit sizes. There are two solutions for this. So-called Euler bends in Si strip waveguides enable low-loss bends down to 1 μm bending radius with less than 0.1 dB/90° loss for both polarizations. Another alternative is a total-internal reflection mirror that can have loss as low as 0.1 dB for both polarizations in either strip or rib waveguides. The excitation of higher order modes in large strip waveguides is avoided by using adiabatic rib-strip converters and low-loss components. With rib and strip waveguides it is possible to reach a unique combination of low loss, extremely small footprint, small polarization dependency, ultra-wide bandwidth and tolerance to high optical powers.

  17. Micron and sub-micron feature replication of amorphous polymers at elevated mold temperature without externally applied pressure

    NASA Astrophysics Data System (ADS)

    Mosaddegh, Peiman; Angstadt, David C.

    2008-03-01

    The focus of this study is on the ability of amorphous polymers to replicate micron and sub-micron features when molded at an elevated mold temperature without externally applied pressure. Molding was performed using three different types of amorphous polymers: cyclo-olefin copolymer (COC), polystyrene (PS) and poly (methyl methacrylate) (PMMA) on a silicon mold containing surface features as small as 700 nm in depth and aspect ratios ranging from 5 to 0.02. In this study, processing temperatures were selected in order to match the viscosity for all polymers used. Polymer viscosity was characterized via cone and plate rheometry and wettability was characterized via contact angle analysis to quantify interfacial effects. Feature replication was assessed using scanning electron microscopy (SEM) and atomic force microscopy (AFM) to compare the molded feature depth ratio. It was observed that for the features with an aspect ratio (depth/width) bigger than 2 the depth ratio of the molded parts decreases. PS shows the best replication because of high wettability behavior. PMMA shows the intermediate replication because of dipole-dipole interaction and its lower diffusion coefficient than PS. COC has the worse replication especially in low aspect ratio because of sticking to the silicon oxide layer. PS has the best surface roughness among all polymers.

  18. Two-Colors Photo-detector Based on Intersubband Transitions in Strained In_0.07Ga_0.93As/Al_0.40Ga_0.60As Multiple Quantum Wells.

    NASA Astrophysics Data System (ADS)

    Huang, D.; Manasreh, M. O.

    1996-03-01

    The absorption spectrum is calculated to study the intersubband transitions of conduction electrons in a Si-doped In_0.07Ga_0.93As/Al_0.40Ga_0.60As quantum well. The selective doping is restricted only in the well region so as to avoid the bending of the conduction bandedge in the well and barriers. The chemical potential determined by the electron density is designed to be above the second subband edge so that two absorption peaks with different wavelength will show up at the same time to fit the requirement of a two-colors infrared photo-detectors. The calculated absorption spectrum is compared with the experimental data for different well widths. The observed temperature behavior in the absorption spectrum is theoretically reproduced and explained. The anti-crossing of two absorption peaks due to the occurrence of a quasi-bound state is predicted. In terms of this theoretical analysis, the optimal condition for the design of a two-colors photo-detector is obtained.

  19. The 2-micron plasmid as a nonselectable, stable, high copy number yeast vector

    NASA Technical Reports Server (NTRS)

    Ludwig, D. L.; Bruschi, C. V.

    1991-01-01

    The endogenous 2-microns plasmid of Saccharomyces cerevisiae has been used extensively for the construction of yeast cloning and expression plasmids because it is a native yeast plasmid that is able to be maintained stably in cells at high copy number. Almost invariably, these plasmid constructs, containing some or all 2-microns sequences, exhibit copy number levels lower than 2-microns and are maintained stably only under selective conditions. We were interested in determining if there was a means by which 2-microns could be utilized for vector construction, without forfeiting either copy number or nonselective stability. We identified sites in the 2-microns plasmid that could be used for the insertion of genetic sequences without disrupting 2-microns coding elements and then assessed subsequent plasmid constructs for stability and copy number in vivo. We demonstrate the utility of a previously described 2-microns recombination chimera, pBH-2L, for the manipulation and transformation of 2-microns as a pure yeast plasmid vector. We show that the HpaI site near the STB element in the 2-microns plasmid can be utilized to clone yeast DNA of at least 3.9 kb with no loss of plasmid stability. Additionally, the copy number of these constructs is as high as levels reported for the endogenous 2-microns.

  20. The 2-micron plasmid as a nonselectable, stable, high copy number yeast vector

    NASA Technical Reports Server (NTRS)

    Ludwig, D. L.; Bruschi, C. V.

    1991-01-01

    The endogenous 2-microns plasmid of Saccharomyces cerevisiae has been used extensively for the construction of yeast cloning and expression plasmids because it is a native yeast plasmid that is able to be maintained stably in cells at high copy number. Almost invariably, these plasmid constructs, containing some or all 2-microns sequences, exhibit copy number levels lower than 2-microns and are maintained stably only under selective conditions. We were interested in determining if there was a means by which 2-microns could be utilized for vector construction, without forfeiting either copy number or nonselective stability. We identified sites in the 2-microns plasmid that could be used for the insertion of genetic sequences without disrupting 2-microns coding elements and then assessed subsequent plasmid constructs for stability and copy number in vivo. We demonstrate the utility of a previously described 2-microns recombination chimera, pBH-2L, for the manipulation and transformation of 2-microns as a pure yeast plasmid vector. We show that the HpaI site near the STB element in the 2-microns plasmid can be utilized to clone yeast DNA of at least 3.9 kb with no loss of plasmid stability. Additionally, the copy number of these constructs is as high as levels reported for the endogenous 2-microns.