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Sample records for 1a parte calidad

  1. 40 CFR Table 1a to Subpart G of... - Applicable 40 CFR Part 63 General Provisions

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 9 2010-07-01 2010-07-01 false Applicable 40 CFR Part 63 General.... 63, Subpt. G, Table 1A Table 1A to Subpart G of Part 63—Applicable 40 CFR Part 63 General Provisions 40 CFR part 63, subpart A, provisions applicable to subpart G § 63.1(a)(1), (a)(2), (a)(3),...

  2. 40 CFR Table 1a to Subpart G of... - Applicable 40 CFR Part 63 General Provisions

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Chemical Manufacturing Industry for Process Vents, Storage Vessels, Transfer Operations, and Wastewater Pt. 63, Subpt. G, Table 1A Table 1A to Subpart G of Part 63—Applicable 40 CFR Part 63 General Provisions... 40 Protection of Environment 10 2013-07-01 2013-07-01 false Applicable 40 CFR Part 63...

  3. 40 CFR Table 1a to Subpart G of... - Applicable 40 CFR Part 63 General Provisions

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Chemical Manufacturing Industry for Process Vents, Storage Vessels, Transfer Operations, and Wastewater Pt. 63, Subpt. G, Table 1A Table 1A to Subpart G of Part 63—Applicable 40 CFR Part 63 General Provisions... 40 Protection of Environment 10 2014-07-01 2014-07-01 false Applicable 40 CFR Part 63...

  4. 40 CFR Table 1a to Subpart G of... - Applicable 40 CFR Part 63 General Provisions

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Chemical Manufacturing Industry for Process Vents, Storage Vessels, Transfer Operations, and Wastewater Pt. 63, Subpt. G, Table 1A Table 1A to Subpart G of Part 63—Applicable 40 CFR Part 63 General Provisions... 40 Protection of Environment 10 2012-07-01 2012-07-01 false Applicable 40 CFR Part 63...

  5. 40 CFR Table 1a to Subpart G of... - Applicable 40 CFR Part 63 General Provisions

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Chemical Manufacturing Industry for Process Vents, Storage Vessels, Transfer Operations, and Wastewater Pt. 63, Subpt. G, Table 1A Table 1A to Subpart G of Part 63—Applicable 40 CFR Part 63 General Provisions... 40 Protection of Environment 9 2011-07-01 2011-07-01 false Applicable 40 CFR Part 63...

  6. Culture and Literacy. Part 1: A Historic Overview. Part 2: The Needs of a Pluralistic Society. Part 3: Student Perceptions.

    ERIC Educational Resources Information Center

    Masters, Billie Nave

    This three-part series of articles discusses restructuring U.S. public education to meet the needs of culturally diverse student populations. Part 1 presents a historic overview of the philosophies and attitudes underlying delivery of U.S. public education. The American school system is slow to change and, for the benefit of the industrialized…

  7. 19 CFR Appendix to Part 163 - Interim (a)(1)(A) List

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... merchandise entered in accordance with the provisions of the sections of 19 CFR listed). These are in addition... preferential tariff treatment, including an importer's certification 15 CFR part 301Requirements for entry of... Federal Agency Records (See 19 CFR part 12, 19 U.S.C. 1484, 1499) Licenses, Authorizations,...

  8. 50 CFR Table 1a to Part 679 - Delivery Condition* and Product Codes

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    .... Throat, or isthmus, slit to allow blood to drain 03 Bled fish destined for fish meal (includes offsite production) DO NOT RECORD ON PTR 42 Bones (if meal, report as 32) (ancillary only) 39 Butterfly, no backbone... in front of tail 21 Fish meal. Meal from whole fish or fish parts; includes bone meal 32 Fish...

  9. 50 CFR Table 1a to Part 679 - Delivery Condition and Product Codes

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Bled only. Throat or isthmus slit to allow blood to drain. 03 Bled fish destined for fish meal... Cheeks. Muscles on sides of head (ancillary only). 17 Chins. Lower jaw (mandible), muscles, and flesh... body behind head and in front of tail. 21 Fish meal. Meal from whole fish or fish parts; includes...

  10. 19 CFR Appendix to Part 163 - Interim (a)(1)(A) List

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Customs: §§ 141.11 through 141.15Evidence of right to make entry (airway bill/bill of lading or *carrier... merchandise entered in accordance with the provisions of the sections of 19 CFR listed). These are in addition... and relief equipment 15 CFR part 301Requirements for entry of scientific and educational...

  11. Plant-based medicines for anxiety disorders, Part 1: a review of preclinical studies.

    PubMed

    Sarris, Jerome; McIntyre, Erica; Camfield, David A

    2013-03-01

    Research in the area of herbal psychopharmacology has revealed a variety of promising medicines that may provide benefit in the treatment of general anxiety and specific anxiety disorders. However, a comprehensive review of plant-based anxiolytics has been absent to date. This article (part 1) reviews herbal medicines for which only preclinical investigations for anxiolytic activity have been performed. In part 2, we review herbal medicines for which there have been clinical investigations for anxiolytic activity. An open-ended, language-restricted (English) search of MEDLINE (PubMed), CINAHL, Scopus and the Cochrane Library databases was conducted (up to 28 October 2012) using specific search criteria to identify herbal medicines that have been investigated for anxiolytic activity. This search of the literature revealed 1,525 papers, from which 53 herbal medicines were included in the full review (having at least one study using the whole plant extract). Of these plants, 21 had human clinical trial evidence (reviewed in part 2), with another 32 having solely preclinical studies (reviewed here in part 1). Preclinical evidence of anxiolytic activity (without human clinical trials) was found for Albizia julibrissin, Sonchus oleraceus, Uncaria rhynchophylla, Stachys lavandulifolia, Cecropia glazioui, Magnolia spp., Eschscholzia californica, Erythrina spp., Annona spp., Rubus brasiliensis, Apocynum venetum, Nauclea latifolia, Equisetum arvense, Tilia spp., Securidaca longepedunculata, Achillea millefolium, Leea indica, Juncus effusus, Coriandrum sativum, Eurycoma longifolia, Turnera diffusa, Euphorbia hirta, Justicia spp., Crocus sativus, Aloysia polystachya, Albies pindrow, Casimiroa edulis, Davilla rugosa, Gastrodia elata, Sphaerathus indicus, Zizyphus jujuba and Panax ginseng. Common mechanisms of action for the majority of botanicals reviewed primarily involve GABA, either via direct receptor binding or ionic channel or cell membrane modulation; GABA transaminase

  12. Plant-based medicines for anxiety disorders, Part 1: a review of preclinical studies.

    PubMed

    Sarris, Jerome; McIntyre, Erica; Camfield, David A

    2013-03-01

    Research in the area of herbal psychopharmacology has revealed a variety of promising medicines that may provide benefit in the treatment of general anxiety and specific anxiety disorders. However, a comprehensive review of plant-based anxiolytics has been absent to date. This article (part 1) reviews herbal medicines for which only preclinical investigations for anxiolytic activity have been performed. In part 2, we review herbal medicines for which there have been clinical investigations for anxiolytic activity. An open-ended, language-restricted (English) search of MEDLINE (PubMed), CINAHL, Scopus and the Cochrane Library databases was conducted (up to 28 October 2012) using specific search criteria to identify herbal medicines that have been investigated for anxiolytic activity. This search of the literature revealed 1,525 papers, from which 53 herbal medicines were included in the full review (having at least one study using the whole plant extract). Of these plants, 21 had human clinical trial evidence (reviewed in part 2), with another 32 having solely preclinical studies (reviewed here in part 1). Preclinical evidence of anxiolytic activity (without human clinical trials) was found for Albizia julibrissin, Sonchus oleraceus, Uncaria rhynchophylla, Stachys lavandulifolia, Cecropia glazioui, Magnolia spp., Eschscholzia californica, Erythrina spp., Annona spp., Rubus brasiliensis, Apocynum venetum, Nauclea latifolia, Equisetum arvense, Tilia spp., Securidaca longepedunculata, Achillea millefolium, Leea indica, Juncus effusus, Coriandrum sativum, Eurycoma longifolia, Turnera diffusa, Euphorbia hirta, Justicia spp., Crocus sativus, Aloysia polystachya, Albies pindrow, Casimiroa edulis, Davilla rugosa, Gastrodia elata, Sphaerathus indicus, Zizyphus jujuba and Panax ginseng. Common mechanisms of action for the majority of botanicals reviewed primarily involve GABA, either via direct receptor binding or ionic channel or cell membrane modulation; GABA transaminase

  13. Long-term athletic development- part 1: a pathway for all youth.

    PubMed

    Lloyd, Rhodri S; Oliver, Jon L; Faigenbaum, Avery D; Howard, Rick; De Ste Croix, Mark B A; Williams, Craig A; Best, Thomas M; Alvar, Brent A; Micheli, Lyle J; Thomas, D Phillip; Hatfield, Disa L; Cronin, John B; Myer, Gregory D

    2015-05-01

    The concept of developing talent and athleticism in youth is the goal of many coaches and sports systems. Consequently, an increasing number of sporting organizations have adopted long-term athletic development models in an attempt to provide a structured approach to the training of youth. It is clear that maximizing sporting talent is an important goal of long-term athletic development models. However, ensuring that youth of all ages and abilities are provided with a strategic plan for the development of their health and physical fitness is also important to maximize physical activity participation rates, reduce the risk of sport- and activity-related injury, and to ensure long-term health and well-being. Critical reviews of independent models of long-term athletic development are already present within the literature; however, to the best of our knowledge, a comprehensive examination and review of the most prominent models does not exist. Additionally, considerations of modern day issues that may impact on the success of any long-term athletic development model are lacking, as are proposed solutions to address such issues. Therefore, within this 2-part commentary, Part 1 provides a critical review of existing models of practice for long-term athletic development and introduces a composite youth development model that includes the integration of talent, psychosocial and physical development across maturation. Part 2 identifies limiting factors that may restrict the success of such models and offers potential solutions.

  14. Long-term athletic development- part 1: a pathway for all youth.

    PubMed

    Lloyd, Rhodri S; Oliver, Jon L; Faigenbaum, Avery D; Howard, Rick; De Ste Croix, Mark B A; Williams, Craig A; Best, Thomas M; Alvar, Brent A; Micheli, Lyle J; Thomas, D Phillip; Hatfield, Disa L; Cronin, John B; Myer, Gregory D

    2015-05-01

    The concept of developing talent and athleticism in youth is the goal of many coaches and sports systems. Consequently, an increasing number of sporting organizations have adopted long-term athletic development models in an attempt to provide a structured approach to the training of youth. It is clear that maximizing sporting talent is an important goal of long-term athletic development models. However, ensuring that youth of all ages and abilities are provided with a strategic plan for the development of their health and physical fitness is also important to maximize physical activity participation rates, reduce the risk of sport- and activity-related injury, and to ensure long-term health and well-being. Critical reviews of independent models of long-term athletic development are already present within the literature; however, to the best of our knowledge, a comprehensive examination and review of the most prominent models does not exist. Additionally, considerations of modern day issues that may impact on the success of any long-term athletic development model are lacking, as are proposed solutions to address such issues. Therefore, within this 2-part commentary, Part 1 provides a critical review of existing models of practice for long-term athletic development and introduces a composite youth development model that includes the integration of talent, psychosocial and physical development across maturation. Part 2 identifies limiting factors that may restrict the success of such models and offers potential solutions. PMID:25486295

  15. What's law got to do with it part 1: A framework for obesity prevention

    PubMed Central

    Magnusson, Roger S

    2008-01-01

    This article provides a conceptual framework for thinking about the role of law in responding to population weight gain in Australia. Part 1 focuses on two core questions. Firstly, in pursuing the aim of weight reduction at the population level, what should law be trying to influence? The challenge here is to identify a model of the determinants of obesity that is adequate for legal purposes and that illustrates the entry points where law could best be used as an instrument of public health policy. Secondly, what kinds of strategies and tools can law offer to obesity prevention? The challenge here is to identify a model of law that captures the variety of contributions law is capable of making, at different levels of government, and across different legal systems. In Part 1 of the article, I argue that although law can intervene at a number of levels, the most important opportunities lie in seeking to influence the social, economic and environmental influences that shape patterns of eating and nutrition across the population as a whole. Only policies that impact broadly across the population can be expected to influence the weight distribution curve that has shifted relentlessly to the right in recent decades. Part 2 of the article builds on this analysis by offering a critical review of selected legal strategies for healthier nutrition and obesity prevention. PMID:18533998

  16. Eigen analysis of tree-ring records: Part 1, a limited representativeness of regional curve

    NASA Astrophysics Data System (ADS)

    Yang, Bao; Sonechkin, Dmitry M.; Datsenko, Nina M.; Ivashchenko, Nadezda N.; Liu, Jingjing; Qin, Chun

    2011-12-01

    Based on a set of very long-living (2,000 years) Qilian junipers ( Sabina przewalskii Kom.) from the north-eastern part of the Tibetan Plateau (the region of Dulan), we carefully consider the regional curve standardization (RCS) technique. For this goal, we correlate deviations of individual tree-ring width records from their regional mean age-dependent curve (RC). It turns out that these correlations keep their positivity for almost all shifts between ages compared (up to 500 years and even more) evidencing each Dulan juniper to be a unique "thermometer". Just the unification of these "thermometers" in the form RC creates a spurious positive trend in the Dulan chronology. We modify the RCS technique to closer attach RC to these "thermometers" in order to construct a new chronology in which the trend is absent.

  17. Psychiatry training in the United Kingdom--Part 1: a general overview.

    PubMed

    Kasiakogia, K; Christodoulou, N

    2014-01-01

    In recent years there has been a strong trend of emigration of Greek medical doctors. The reason for this phenomenon is certainly multifactorial, but it has been greatly exacerbated due to the latest financial crisis. The United Kingdom is one of the most popular destinations amongst emigrating Greek psychiatric doctors, as reflected by official data and by the sheer volume of requests for information received by the United Kingdom Division of the Hellenic Psychiatric Association. There are many systemic and practical differences between the Greek and the United Kingdom health systems, which complicate training and further career decisions. These complex differences make it hard for psychiatric doctors to decide which steps to take, and often result in them making the "wrong" decision. These "wrong" decisions are very often the result of poor information or misinformation. For instance many doctors are confused about the equivalence of training and service grades between Greece and the United Kingdom, what a good portfolio means, or the significance of the MRCPsych exam. This information exists, sometimes in comprehensive ways on the internet, but for doctors who are not familiar with the system, finding this information can be a time-consuming and laborious task. Therefore, providing a starting point with realistic and useful information about psychiatric training and generally career progression in the United Kingdom to Greek psychiatric doctors has become very important. The United Kingdom Division of the Hellenic Psychiatric Association has decided to pick up the role of providing exactly that information. The first part of this two-piece paper provides a starting point for Greek doctors considering the move to the United Kingdom for training and/or work in psychiatry. Firstly, it gives a general overview of psychiatric training in the United Kingdom, and explains that the pragmatic equivalence between training stages between Greece and the United Kingdom often

  18. Steady induction effects in geomagnetism. Part 1A: Steady motional induction of geomagnetic chaos

    NASA Technical Reports Server (NTRS)

    Voorhies, Coerte V.

    1992-01-01

    Geomagnetic effects of magnetic induction by hypothetically steady fluid motion and steady magnetic flux diffusion near the top of Earth's core are investigated using electromagnetic theory, simple magnetic earth models, and numerical experiments with geomagnetic field models. The problem of estimating a steady fluid velocity field near the top of Earth's core which induces the secular variation indicated by broad-scale models of the observed geomagnetic field is examined and solved. In Part 1, the steady surficial core flow estimation problem is solved in the context of the source-free mantle/frozen-flux core model. In the first paper (IA), the theory underlying such estimates is reviewed and some consequences of various kinematic and dynamic flow hypotheses are derived. For a frozen-flux core, fluid downwelling is required to change the mean square normal magnetic flux density averaged over the core-mantle boundary. For surficially geostrophic flow, downwelling implies poleward flow. The solution of the forward steady motional induction problem at the surface of a frozen-flux core is derived and found to be a fine, easily visualized example of deterministic chaos. Geomagnetic effects of statistically steady core surface flow may well dominate secular variation over several decades. Indeed, effects of persistent, if not steady, surficially geostrophic core flow are described which may help explain certain features of the present broad-scale geomagnetic field and perhaps paleomagnetic secular variation.

  19. Vitamin D as an adjunctive therapy in asthma. Part 1: A review of potential mechanisms.

    PubMed

    Kerley, Conor P; Elnazir, Basil; Faul, John; Cormican, Liam

    2015-06-01

    Vitamin D deficiency (VDD) is highly prevalent worldwide. The classical role for vitamin D is to regulate calcium absorption form the gastrointestinal tract and influence bone health. Recently vitamin D receptors and vitamin D metabolic enzymes have been discovered in numerous sites systemically supporting diverse extra-skeletal roles of vitamin D, for example in asthmatic disease. Further, VDD and asthma share several common risk factors including high latitude, winter season, industrialization, poor diet, obesity, and dark skin pigmentation. Vitamin D has been demonstrated to possess potent immunomodulatory effects, including effects on T cells and B cells as well as increasing production of antimicrobial peptides (e.g. cathelicidin). This immunomodulation may lead to asthma specific clinical benefits in terms of decreased bacterial/viral infections, altered airway smooth muscle-remodeling and -function as well as modulation of response to standard anti-asthma therapy (e.g. glucocorticoids and immunotherapy). Thus, vitamin D and its deficiency have a number of biological effects that are potentially important in altering the course of disease pathogenesis and severity in asthma. The purpose of this first of a two-part review is to review potential mechanisms whereby altering vitamin D status may influence asthmatic disease.

  20. 12 CFR Appendix A to Part 1024 - Instructions for Completing HUD-1 and HUD-1a Settlement Statements; Sample HUD-1 and HUD-1a...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... statement, required under section 4 of RESPA and 12 CFR part 1024 (Regulation X) of the Bureau of Consumer.... Refer to the definitions section of the regulations (12 CFR 1024.2) for specific definitions of many of... user. For other loans covered by 12 CFR part 1024 (Regulation X) which finance construction of a...

  1. 12 CFR Appendix A to Part 1024 - Instructions for Completing HUD-1 and HUD-1A Settlement Statements; Sample HUD-1 and HUD-1A...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... statement, required under section 4 of RESPA and 12 CFR part 1024 (Regulation X) of the Bureau of Consumer.... Refer to the definitions section of the regulations (12 CFR 1024.2) for specific definitions of many of... user. For other loans covered by 12 CFR part 1024 (Regulation X) which finance construction of a...

  2. 12 CFR Appendix A to Part 1024 - Instructions for Completing HUD-1 and HUD-1a Settlement Statements; Sample HUD-1 and HUD-1a...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... statement, required under section 4 of RESPA and 12 CFR part 1024 (Regulation X) of the Bureau of Consumer.... Refer to the definitions section of the regulations (12 CFR 1024.2) for specific definitions of many of... user. For other loans covered by 12 CFR part 1024 (Regulation X) which finance construction of a...

  3. 24 CFR Appendix A to Part 3500 - Instructions for Completing HUD-1 and HUD-1a Settlement Statements; Sample HUD-1 and HUD-1a...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... instructions for completing the HUD-1 settlement statement, required under section 4 of RESPA and 24 CFR part... (24 CFR 3500.2) for specific definitions of many of the terms that are used in these instructions... is converted to a loan to finance purchase by the first user. For other loans covered by 24 CFR...

  4. 24 CFR Appendix A to Part 3500 - Instructions for Completing HUD-1 and HUD-1a Settlement Statements; Sample HUD-1 and HUD-1a...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... instructions for completing the HUD-1 settlement statement, required under section 4 of RESPA and 24 CFR part... (24 CFR 3500.2) for specific definitions of many of the terms that are used in these instructions... is converted to a loan to finance purchase by the first user. For other loans covered by 24 CFR...

  5. 24 CFR Appendix A to Part 3500 - Instructions for Completing HUD-1 and HUD-1a Settlement Statements; Sample HUD-1 and HUD-1a...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... instructions for completing the HUD-1 settlement statement, required under section 4 of RESPA and 24 CFR part... (24 CFR 3500.2) for specific definitions of many of the terms that are used in these instructions... is converted to a loan to finance purchase by the first user. For other loans covered by 24 CFR...

  6. 24 CFR Appendix A to Part 3500 - Instructions for Completing HUD-1 and HUD-1a Settlement Statements; Sample HUD-1 and HUD-1a...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... instructions for completing the HUD-1 settlement statement, required under section 4 of RESPA and 24 CFR part... (24 CFR 3500.2) for specific definitions of many of the terms that are used in these instructions... is converted to a loan to finance purchase by the first user. For other loans covered by 24 CFR...

  7. 24 CFR Appendix A to Part 3500 - Instructions for Completing HUD-1 and HUD-1a Settlement Statements; Sample HUD-1 and HUD-1a...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... instructions for completing the HUD-1 settlement statement, required under section 4 of RESPA and 24 CFR part... (24 CFR 3500.2) for specific definitions of many of the terms that are used in these instructions... is converted to a loan to finance purchase by the first user. For other loans covered by 24 CFR...

  8. Part-Time Students and Part-Time Study in Higher Education in the UK: Strand 1--A Quantitative Data Analysis of 2003/04 HESA Data

    ERIC Educational Resources Information Center

    Ramsden, Brian

    2006-01-01

    This is a report of the Strand 1 element of the Universities UK project to examine part-time students and part-time study within the United Kingdom. The aim of Strand 1 of this project is to set out, as far as possible, factual information about students studying part-time and following programmes of study leading to higher education…

  9. 50 CFR Table 1a to Part 660... - 2009, Specifications of ABCs, OYs, and HGs, by Management Area (weights in metric tons)

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., by Management Area (weights in metric tons) 1a Table 1a to Part 660, Subpart G Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF..., Subpart G—2009, Specifications of ABCs, OYs, and HGs, by Management Area (weights in metric tons) Link...

  10. 50 CFR Table 1a to Part 660... - 2009, Specifications of ABCs, OYs, and HGs, by Management Area (weights in metric tons)

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., by Management Area (weights in metric tons) 1a Table 1a to Part 660, Subpart C Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF..., Subpart C—2009, Specifications of ABCs, OYs, and HGs, by Management Area (weights in metric tons)...

  11. 50 CFR Table 1a to Part 660... - 2013, Specifications of OFL, ABC, ACL, ACT and Fishery Harvest guidelines (weights in metric tons)

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 13 2014-10-01 2014-10-01 false 2013, Specifications of OFL, ABC, ACL, ACT and Fishery Harvest guidelines (weights in metric tons) 1a Table 1a to Part 660, Subpart C Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES...

  12. 50 CFR Table 1a to Part 660... - 2013, Specifications of OFL, ABC, ACL, ACT and Fishery Harvest guidelines (weights in metric tons)

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 13 2013-10-01 2013-10-01 false 2013, Specifications of OFL, ABC, ACL, ACT and Fishery Harvest guidelines (weights in metric tons) 1a Table 1a to Part 660, Subpart C Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES...

  13. Elongation Factor 1A-1 Is a Mediator of Hepatocyte Lipotoxicity Partly through Its Canonical Function in Protein Synthesis

    PubMed Central

    Stoianov, Alexandra M.; Robson, Debra L.; Hetherington, Alexandra M.; Sawyez, Cynthia G.; Borradaile, Nica M.

    2015-01-01

    Elongation factor 1A-1 (eEF1A-1) has non-canonical functions in regulation of the actin cytoskeleton and apoptosis. It was previously identified through a promoter-trap screen as a mediator of fatty acid-induced cell death (lipotoxicity), and was found to participate in this process downstream of ER stress. Since ER stress is implicated in the pathogenesis of nonalcoholic fatty liver disease (NAFLD), we investigated the mechanism of action of eEF1A-1 in hepatocyte lipotoxicity. HepG2 cells were exposed to excess fatty acids, followed by assessments of ER stress, subcellular localization of eEF1A-1, and cell death. A specific inhibitor of eEF1A-1 elongation activity, didemnin B, was used to determine whether its function in protein synthesis is involved in lipotoxicity. Within 6 h, eEF1A-1 protein was modestly induced by high palmitate, and partially re-localized from its predominant location at the ER to polymerized actin at the cell periphery. This early induction and subcellular redistribution of eEF1A-1 coincided with the onset of ER stress, and was later followed by cell death. Didemnin B did not prevent the initiation of ER stress by high palmitate, as indicated by eIF2α phosphorylation. However, consistent with sustained inhibition of eEF1A-1-dependent elongation activity, didemnin B prevented the recovery of protein synthesis and increase in GRP78 protein that are normally associated with later phases of the response to ongoing ER stress. This resulted in decreased palmitate-induced cell death. Our data implicate eEF1A-1, and its function in protein synthesis, in hepatocyte lipotoxicity. PMID:26102086

  14. Design and synthesis of thiazolo[5,4-f]quinazolines as DYRK1A inhibitors, part II.

    PubMed

    Foucourt, Alicia; Hédou, Damien; Dubouilh-Benard, Carole; Girard, Angélique; Taverne, Thierry; Casagrande, Anne-Sophie; Désiré, Laurent; Leblond, Bertrand; Besson, Thierry

    2014-01-01

    The convenient synthesis of a focused library (forty molecules) of novel 6,6,5-tricyclic thiazolo[5,4-f]quinazolines was realized mainly under microwave irradiation. A novel 6-aminobenzo[d]thiazole-2,7-dicarbonitrile (1) was used as a versatile molecular platform for the synthesis of various derivatives. Kinase inhibition, of the obtained final compounds, was evaluated on a panel of two kinases (DYRK1A/1B) together with some known reference DYRK1A and DYRK1B inhibitors (harmine, TG003, NCGC-00189310 and leucettine L41). Compound IC50 values were obtained and compared. Five of the novel thiazolo[5,4-f]quinazoline derivatives prepared, EHT 5372 (8c), EHT 6840 (8h), EHT 1610 (8i), EHT 9851 (8k) and EHT 3356 (9b) displayed single-digit nanomolar or subnanomolar IC50 values and are among the most potent DYRK1A/1B inhibitors disclosed to date. DYRK1A/1B kinases are known to be involved in the regulation of various molecular pathways associated with oncology, neurodegenerative diseases (such as Alzheimer disease, AD, or other tauopathies), genetic diseases (such as Down Syndrome, DS), as well as diseases involved in abnormal pre-mRNA splicing. The compounds described in this communication constitute a highly potent set of novel molecular probes to evaluate the biology/pharmacology of DYR1A/1B in such diseases. PMID:25264830

  15. Benchmarking the performance of fixed-image receptor digital radiographic systems part 1: a novel method for image quality analysis.

    PubMed

    Lee, Kam L; Ireland, Timothy A; Bernardo, Michael

    2016-06-01

    This is the first part of a two-part study in benchmarking the performance of fixed digital radiographic general X-ray systems. This paper concentrates on reporting findings related to quantitative analysis techniques used to establish comparative image quality metrics. A systematic technical comparison of the evaluated systems is presented in part two of this study. A novel quantitative image quality analysis method is presented with technical considerations addressed for peer review. The novel method was applied to seven general radiographic systems with four different makes of radiographic image receptor (12 image receptors in total). For the System Modulation Transfer Function (sMTF), the use of grid was found to reduce veiling glare and decrease roll-off. The major contributor in sMTF degradation was found to be focal spot blurring. For the System Normalised Noise Power Spectrum (sNNPS), it was found that all systems examined had similar sNNPS responses. A mathematical model is presented to explain how the use of stationary grid may cause a difference between horizontal and vertical sNNPS responses.

  16. Design and synthesis of thiazolo[5,4-f]quinazolines as DYRK1A inhibitors, part I.

    PubMed

    Foucourt, Alicia; Hédou, Damien; Dubouilh-Benard, Carole; Désiré, Laurent; Casagrande, Anne-Sophie; Leblond, Bertrand; Loäec, Nadège; Meijer, Laurent; Besson, Thierry

    2014-01-01

    The convenient synthesis of a library of novel 6,6,5-tricyclic thiazolo[5,4-f] quinazolines (forty molecules) was achieved mainly under microwave irradiation. Dimroth rearrangement and 4,5-dichloro-1,2,3,-dithiazolium chloride (Appel salt) chemistry were associated for the synthesis of a novel 6-aminobenzo[d]thiazole-2,7-dicarbonitrile (16) a versatile molecular platform for the synthesis of various bioactive derivatives. Kinase inhibition of the final compounds was evaluated on a panel of four Ser/Thr kinases (DYRK1A, CDK5, CK1 and GSK3) chosen for their strong implications in various regulation processes, especially Alzheimer's disease (AD). In view of the results of this preliminary screening, thiazolo[5,4-f]quinazoline scaffolds constitutes a promising source of inspiration for the synthesis of novel bioactive molecules. Among the compounds of this novel chemolibrary, 7i, 8i and 9i inhibited DYRK1A with IC50 values ranging in the double-digit nanomolar range (40, 47 and 50 nM, respectively). PMID:25268714

  17. Parental experiences of providing skin-to-skin care to their newborn infant—Part 1: A qualitative systematic review

    PubMed Central

    Anderzén-Carlsson, Agneta; Lamy, Zeni Carvalho; Eriksson, Mats

    2014-01-01

    Aim To describe parental experiences of providing skin-to-skin care (SSC) to their newborn infants. Background SSC care for newborn infants has been reported to have positive physiological and psychological benefits to the infants and their parents. No systematic review regarding parental experiences has been identified. Design In this first part of a meta-study, the findings of a systematic literature review on parental experience of SSC care are presented. Data sources Four databases were searched, without year or language limitations, up until December 2013. Manual searches were performed in reference lists and in a bibliography of the topic. Review methods After a quality-appraisal process, data from the original articles were extracted and analysed using qualitative content analysis. Results The systematic and manual searches led to the inclusion of 29 original qualitative papers from nine countries, reporting experiences from 401 mothers and 94 fathers. Two themes that characterized the provision of SSC emerged: a restoring experience and an energy-draining experience. Conclusion This review has added scientific and systematic knowledge about parental experiences of providing SSC. Further research about fathers’ experiences is recommended. PMID:25319746

  18. The representation of solar cycle signals in stratospheric ozone - Part 1: A comparison of recently updated satellite observations

    NASA Astrophysics Data System (ADS)

    Maycock, Amanda C.; Matthes, Katja; Tegtmeier, Susann; Thiéblemont, Rémi; Hood, Lon

    2016-08-01

    Changes in incoming solar ultraviolet radiation over the 11-year solar cycle affect stratospheric ozone abundances. It is important to quantify the magnitude, structure, and seasonality of the associated solar-ozone response (SOR) to understand the impact of the 11-year solar cycle on climate. Part 1 of this two-part study uses multiple linear regression analysis to extract the SOR in a number of recently updated satellite ozone datasets covering different periods within the epoch 1970 to 2013. The annual mean SOR in the updated version 7.0 (v7.0) Stratospheric Aerosol and Gas Experiment (SAGE) II number density dataset (1984-2004) is very consistent with that found in the previous v6.2. In contrast, we find a substantial decrease in the magnitude of the SOR in the tropical upper stratosphere in the SAGE II v7.0 mixing ratio dataset (˜ 1 %) compared to the v6.2 (˜ 4 %). This difference is shown to be largely attributable to the change in the independent stratospheric temperature dataset used to convert SAGE II ozone number densities to mixing ratios. Since these temperature records contain substantial uncertainties, we suggest that datasets based on SAGE II number densities are currently most reliable for evaluating the SOR. We further analyse three extended ozone datasets that combine SAGE II v7.0 number densities with more recent GOMOS (Global Ozone Monitoring by Occultation of Stars) or OSIRIS (Optical Spectrograph and Infrared Imager System) measurements. The extended SAGE-OSIRIS dataset (1984-2013) shows a smaller and less statistically significant SOR across much of the tropical upper stratosphere compared to the SAGE II data alone. In contrast, the two SAGE-GOMOS datasets (1984-2011) show SORs that are in closer agreement with the original SAGE II data and therefore appear to provide a more reliable estimate of the SOR. We also analyse the SOR in the recent Solar Backscatter Ultraviolet Instrument (SBUV) Merged Ozone Dataset (SBUVMOD) version 8.6 (VN8

  19. Part 1. A time-series study of ambient air pollution and daily mortality in Shanghai, China.

    PubMed

    Kan, Haidong; Chen, Bingheng; Zhao, Naiqing; London, Stephanie J; Song, Guixiang; Chen, Guohai; Zhang, Yunhui; Jiang, Lili

    2010-11-01

    Although the relation between outdoor air pollution and daily mortality has been examined in several Chinese cities, there are still a number of key scientific issues to be addressed concerning the health effects of air pollution in China. Given the changes over the past decade in concentrations and sources of air pollution (e.g., the change from one predominant source [coal combustion], which was typical of the twentieth century, to a mix of sources [coal combustion and motor-vehicle emissions]) and transition in China, it is worthwhile to investigate the acute effects of outdoor air pollution on mortality outcomes in the country. We conducted a time-series study to investigate the relation between outdoor air pollution and daily mortality in Shanghai using four years of daily data (2001-2004). This study is a part of the Public Health and Air Pollution in Asia (PAPA) program supported by the Health Effects Institute (HEI). We collected data on daily mortality, air pollution, and weather from the Shanghai Municipal Center of Disease Control and Prevention (SMCDCP), Shanghai Environmental Monitoring Center, and Shanghai Meteorologic Bureau. An independent auditing team assigned by HEI validated all the data. Our statistical analysis followed the Common Protocol of the PAPA program (found at the end of this volume). Briefly, a natural-spline model was used to analyze the mortality, air pollution, and covariate data. We first constructed the basic models for various mortality outcomes excluding variables for air pollution, and used the partial autocorrelation function of the residuals to guide the selection of degrees of freedom for time trend and lag days for the autoregression terms. Thereafter, we introduced the pollutant variables and analyzed their effects on mortality outcomes, including both mortality due to all natural (nonaccidental) causes and cause-specific mortality. We fitted single- and multipollutant models to assess the stability of the effects of the

  20. Calidad de Imagen del Telescopio UNAM212

    NASA Astrophysics Data System (ADS)

    Cobos, F. J.; Teiada de Vargas, C.

    1987-05-01

    El telescopio UNAM2l2, del Observatorio Astronómico Nacional, situado en la Sierra de San Pedro Mártir (Baja California, México), cumplira en un futuro muy cercano siete años de uso para fines de investigación astronómica. Aunque en este tiempo no se ha efectuado un estudio sistemático acerca de su comportamiento óptico y de los factores que influyen en la calidad de las imágenes, se han realizado pruebas diversas, estudios parciales y reuniones especificas, cuyos resultados no siempre se han difundido ampliamente y generalmente no se han presentado por escrito. Es por ello que hemos creido necesario intentar una recopilación de la información existente para poder con ella establecer un diagnóstjco que, aunque no sea definitivo, sirva de base para futuros trabajos tendientes a optimizar el comportamiento óptico del telescopio. Es evidente que un buen número de las conclusiones que se presentan son resultado del trabajo de muchas personas ó de esfuerzos colectivos. Asimismo, hemos tratado de localizar información bibliográfica que pueda ser de utilidad. Nuestro objetivo primordial ha consistido en centrarnos en la óptica del telescopio y su calidad, pero también se han considerado otros aspectos que puedan afectar las imágenes obtenidas tales como: celda del primario, `seeing' local y externo, flexiones posibles en la estructura mecánica del telescopio, etc.

  1. 47 CFR Appendix A to Part 1 - A Plan of Cooperative Procedure in Matters and Cases Under the Provisions of Section 410 of the...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 1 2013-10-01 2013-10-01 false A Plan of Cooperative Procedure in Matters and Cases Under the Provisions of Section 410 of the Communications Act of 1934 A Appendix A to Part 1 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL PRACTICE AND PROCEDURE Pt. 1, App. A Appendix A to Part 1—A Plan of Cooperative...

  2. 47 CFR Appendix A to Part 1 - A Plan of Cooperative Procedure in Matters and Cases Under the Provisions of Section 410 of the...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 1 2011-10-01 2011-10-01 false A Plan of Cooperative Procedure in Matters and Cases Under the Provisions of Section 410 of the Communications Act of 1934 A Appendix A to Part 1 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL PRACTICE AND PROCEDURE Pt. 1, App. A Appendix A to Part 1—A Plan of Cooperative...

  3. Discovery of thienoimidazole-based HCV NS5A inhibitors. Part 2: non-symmetric inhibitors with potent activity against genotype 1a and 1b.

    PubMed

    Giroux, Simon; Bilimoria, Darius; Cadilhac, Caroline; Cottrell, Kevin M; Denis, Francois; Dietrich, Evelyne; Ewing, Nigel; Henderson, James A; L'Heureux, Lucille; Mani, Nagraj; Morris, Mark; Nicolas, Olivier; Reddy, T Jagadeeswar; Selliah, Subajini; Shawgo, Rebecca S; Xu, Jinwang; Chauret, Nathalie; Berlioz-Seux, Francoise; Chan, Laval C; Das, Sanjoy K; Grillot, Anne-Laure; Bennani, Youssef L; Maxwell, John P

    2015-02-15

    The discovery of non-symmetric thienoimidazole-containing HCV NS5A inhibitors is described. The inhibitors herein reported display high potencies against both genotype 1a and 1b. In this follow-up manuscript, we discuss the importance of the linker aromaticity to achieve high potency, particularly against genotype 1a. PMID:25597006

  4. Aeroservoelastic Testing of Free Flying Wind Tunnel Models Part 1: A Sidewall Supported Semispan Model Tested for Gust Load Alleviation and Flutter Suppression

    NASA Technical Reports Server (NTRS)

    Scott, Robert C.; Vetter, Travis K.; Penning, Kevin B.; Coulson, David A.; Heeg, Jennifer.

    2013-01-01

    of a two part document. Part 2 is titled: "Aeroservoelastic Testing of Free Flying Wind Tunnel Models, Part 2: A Centerline Supported Fullspan Model Tested for Gust Load Alleviation." A team comprised of the Air Force Research Laboratory (AFRL), Northrop Grumman, Lockheed Martin, and the NASA Langley Research Center conducted three aeroservoelastic wind tunnel tests in the Transonic Dynamics Tunnel to demonstrate active control technologies relevant to large, flexible vehicles. In the first of these three tests, a semispan, aeroelastically scaled, wind tunnel model of a flying wing SensorCraft vehicle was mounted to a force balance to demonstrate gust load alleviation. In the second and third tests, the same wing was mated to a new, multi-degree of freedom, sidewall mount. This mount allowed the half-span model to translate vertically and pitch at the wing root, allowing better simulation of the full span vehicle's rigid body modes. Gust load alleviation (GLA) and Body freedom flutter (BFF) suppression were successfully demonstrated. The rigid body degrees-of-freedom required that the model be flown in the wind tunnel using an active control system. This risky mode of testing necessitated that a model arrestment system be integrated into the new mount. The safe and successful completion of these free flying tests required the development and integration of custom hardware and software. This paper describes the many systems, software, and procedures that were developed as part of this effort.

  5. 33 CFR 137.70 - Specialized knowledge or experience on the part of persons specified in § 137.1(a).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Specialized knowledge or... INNOCENT LAND-OWNER DEFENSE Standards and Practices § 137.70 Specialized knowledge or experience on the... located, persons specified in § 137.1(a) must take into account their own specialized knowledge of...

  6. Semipermeable membrane devices link site-specific contaminants to effects: Part 1 - Induction of CYP1A in rainbow trout from contaminants in Prince William Sound, Alaska.

    PubMed

    Springman, Kathrine R; Short, Jeffrey W; Lindeberg, Mandy R; Maselko, Jacek M; Khan, Colin; Hodson, Peter V; Rice, Stanley D

    2008-12-01

    Extracts from semi-permeable membrane devices (SPMDs) deployed on beaches in Prince William Sound (PWS), Alaska, were used to evaluate if complex contaminant mixtures from different sources can be distinguished by the resulting cytochrome P450 1A (CYP1A) activity in exposed test animals. Deployment sites included canneries, salmon hatcheries, and beaches where lingering oil remains from discharges during the 1964 earthquake or the 1989 Exxon Valdez oil spill. Other sites were selected at random to evaluate region-wide contaminant inputs or were located in salmon streams to evaluate contaminants carried and released by migrating salmon carcasses following reproduction. Following standard deployments of approximately 28 d, an aliquot of the accumulated contaminants was intraperitoneally injected without cleanup into juvenile rainbow trout (Oncorhynchus mykiss). After 2 d and 7 d, the activity of CYP1A was measured by the ethoxyresorufin-o-deethylase (EROD) assay. Exposure to extracts from the oiled sites and one hatchery site with numerous creosote pilings elicited strong EROD responses, whereas fish exposed to salmon stream extracts elicited weak but significant responses during late summer compared to late spring. Responses from the other sites were not significant, indicating contaminants from these sources are unlikely to cause CYP1A induction in resident biota. Rather than simply assessing extant contaminants, this method evaluates the potency of the different sites for bringing about aryl hydrocarbon receptor responses in resident biota. PMID:18845333

  7. Consensus recommendations from the American Acne & Rosacea Society on the management of rosacea, part 1: a status report on the disease state, general measures, and adjunctive skin care.

    PubMed

    Del Rosso, James Q; Thiboutot, Diane; Gallo, Richard; Webster, Guy; Tanghetti, Emil; Eichenfield, Larry; Stein-Gold, Linda; Berson, Diane; Zaenglein, Andrea

    2013-11-01

    Rosacea is a common clinical diagnosis that encompasses a variety of presentations, predominantly involving the centrofacial skin. Reported to present most commonly in adults of Northern European heritage with fair skin, rosacea can affect males and females of all ethnicities and skin types. Pathophysiologic mechanisms that appear to correlate with the manifestation of rosacea have been the focus of multiple research studies, with outcomes providing a better understanding of why some individuals are affected and how their visible signs and symptoms develop. A better appreciation of the pathophysiologic mechanisms and inflammatory pathways of rosacea has allowed therapeutic strategies to be optimally incorporated. Part 1 of this 5-part series discusses the rosacea disease state with an emphasis on clinical correlation, reviews adjunctive skin care for cutaneous rosacea, and provides management caveats.

  8. High consistency forming process for paper making. Part 1. A research, development, and demonstration program plan for the US Papermaking Industry. Final report

    SciTech Connect

    Not Available

    1980-04-01

    The subject of research, development, and demonstration (RD and D) of energy conserving technologies applicable to papermaking operations downstream of the pulping process is addressed. An RD and D Program Plan is presented based on a survey of leading representatives of the papermaking industry, equipment manufacturers, consulting engineering firms, the American Paper Institute, and the Technical Association of the Pulp and Paper Industry. For perspective, the program plan is presented against the general background of the industry's current issues and concerns. The second part of the paper, Phase I, final report, deals with papermaking test facilities. The case for a centralized test facility is discussed. The results of a survey of existing pilot paper machines are presented. The energy saving potential of high consistency forming is considered and related to existing evidence. Simple theoretical models for the press nip action and the drying process are developed to predict where high consistency forming will reduce energy consumption. A special dynamic former has been designed, fabricated, and commissioned to allow development of a laboratory high consistency headbox. The design and construction of a low speed headbox has been completed and the complete system operated. Special equipment and techniques for the measurement of the water and air permeability of sheet samples have been developed and are described.

  9. Geologic Interpretation of Data Sets Collected by Planetary Analog Geology Traverses and by Standard Geologic Field Mapping. Part 1; A Comparison Study

    NASA Technical Reports Server (NTRS)

    Eppler, Dean B.; Bleacher, Jacob F.; Evans, Cynthia A.; Feng, Wanda; Gruener, John; Hurwitz, Debra M.; Skinner, J. A., Jr.; Whitson, Peggy; Janoiko, Barbara

    2013-01-01

    Geologic maps integrate the distributions, contacts, and compositions of rock and sediment bodies as a means to interpret local to regional formative histories. Applying terrestrial mapping techniques to other planets is challenging because data is collected primarily by orbiting instruments, with infrequent, spatiallylimited in situ human and robotic exploration. Although geologic maps developed using remote data sets and limited "Apollo-style" field access likely contain inaccuracies, the magnitude, type, and occurrence of these are only marginally understood. This project evaluates the interpretative and cartographic accuracy of both field- and remote-based mapping approaches by comparing two 1:24,000 scale geologic maps of the San Francisco Volcanic Field (SFVF), north-central Arizona. The first map is based on traditional field mapping techniques, while the second is based on remote data sets, augmented with limited field observations collected during NASA Desert Research & Technology Studies (RATS) 2010 exercises. The RATS mission used Apollo-style methods not only for pre-mission traverse planning but also to conduct geologic sampling as part of science operation tests. Cross-comparison demonstrates that the Apollo-style map identifies many of the same rock units and determines a similar broad history as the field-based map. However, field mapping techniques allow markedly improved discrimination of map units, particularly unconsolidated surficial deposits, and recognize a more complex eruptive history than was possible using Apollo-style data. Further, the distribution of unconsolidated surface units was more obvious in the remote sensing data to the field team after conducting the fieldwork. The study raises questions about the most effective approach to balancing mission costs with the rate of knowledge capture, suggesting that there is an inflection point in the "knowledge capture curve" beyond which additional resource investment yields progressively

  10. Assessing Subaqueous Mudflow Hazard on the Mississippi River Delta Front, Part 1: A Historical Perspective on Mississippi River Delta Front Sedimentation

    NASA Astrophysics Data System (ADS)

    Maloney, J. M.; Bentley, S. J.; Obelcz, J.; Xu, K.; Miner, M. D.; Georgiou, I. Y.; Hanegan, K.; Keller, G.

    2014-12-01

    potential mudflow hazards associated with new MRDF sedimentation rates and patterns (See Part 2, Obelcz et al.).

  11. Incrementando la calidad de los espectros echelle

    NASA Astrophysics Data System (ADS)

    Pintado, O. I.; Adelman, S. J.

    For more than 10 years we have obtained spectra with the REOSC and EBASIM spectrograph at CASLEO. Usually we use IRAF to extract the spectra. Then we normalized the 1-d spectra and measured the lines with REDUCE. In this paper we compare the results obtained using each program in different parts of the spectrum measurements process. Wih EBASIM, or 4th magnitude stars and 120 minute exposures we obtain signal-to-noise ratios near 500 in the center of the orders

  12. Equal Educational Opportunity: Hearings Before the Select Committee on Equal Educational Opportunity of the United States Senate, Ninety-First Congress, Second Session on Equal Educational Opportunity. Parts 1A, 1B, 2, 3A, 3B, 3C, and 3D.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. Senate Select Committee on Equal Educational Opportunity.

    These hearings before the Senate Select Committee on Equal Educational Opportunity are organized in three parts, the contents of which are as follows: Part 1A and Part 2 comprise the "Introduction," with opening statements by a number of Senators, followed by the presentations of other witnesses. The focus of these two parts is on such topics as…

  13. Vigilando la Calidad del Agua de los Grandes Rios de la Nacion: El Programa NASQAN del Rio Grande (Rio Bravo del Norte)

    USGS Publications Warehouse

    Lurry, Dee L.; Reutter, David C.; Wells, Frank C.; Rivera, M.C.; Munoz, A.

    1998-01-01

    La Oficina del Estudio Geologico de los Estados Unidos (U.S. Geological Survey, 0 USGS) ha monitoreado la calidad del agua de la cuenca del Rio Grande (Rio Bravo del Norte) desde 1995 como parte de la rediseiiada Red Nacional para Contabilizar la Calidad del Agua de los Rios (National Stream Quality Accounting Network, o NASOAN) (Hooper and others, 1997). EI programa NASOAN fue diseiiado para caracterizar las concentraciones y el transporte de sedimento y constituyentes quimicos seleccionados, encontrados en los grandes rios de los Estados Unidos - incluyendo el Misisipi, el Colorado y el Columbia, ademas del Rio Grande. En estas cuatro cuencas, el USGS opera actualmente (1998) una red de 40 puntos de muestreo pertenecientes a NASOAN, con un enfasis en cuantificar el flujo en masa (la cantidad de material que pasa por la estacion, expresado en toneladas por dial para cada constituyente. Aplicacando un enfoque consistente, basado en la cuantificacion de flujos en la cuenca del Rio Grande, el programa NASOAN esta generando la informacion necesaria para identificar fuentes regionales de diversos contaminantes, incluyendo sustancias qui micas agricolas y trazas elementos en la cuenca. EI efecto de las grandes reservas en el Rio Grande se puede observar segun los flujos de constituyentes discurren a 10 largo del rio. EI analisis de los flujos de constituyentes a escala de la cuenca proveera los medios para evaluar la influencia de la actividad humana sobre las condiciones de calidad del agua del Rio Grande.

  14. Semipermeable membrane devices link site-specific contaminants to effects: PART II - A comparison of lingering Exxon Valdez oil with other potential sources of CYP1A inducers in Prince William Sound, Alaska.

    PubMed

    Short, Jeffrey W; Springman, Kathrine R; Lindeberg, Mandy R; Holland, Larry G; Larsen, Marie L; Sloan, Catherine A; Khan, Colin; Hodson, Peter V; Rice, Stanley D

    2008-12-01

    We deployed semipermeable membrane devices (SPMDs) on beaches for 28 days at 53 sites in Prince William Sound (PWS), Alaska, to evaluate the induction potential from suspected sources of cytochrome P450 1A (CYP1A)-inducing contaminants. Sites were selected to assess known point sources, or were chosen randomly to evaluate the region-wide sources. After deployment, SPMD extracts were analyzed chemically for persistent organic pollutants (POPs) and polycyclic aromatic hydrocarbons (PAH). These results were compared with hepatic CYP1A enzyme activity of juvenile rainbow trout injected with the same extracts prior to clean-up for the chemical analyses. Increased CYP1A activity was strongly associated with PAH concentrations in extracts, especially chrysene homologues but was not associated with POPs. The only apparent sources of chrysene homologues were lingering oil from Exxon Valdez, asphalt and bunker fuels released from storage tanks during the 1964 Alaska earthquake, creosote leaching from numerous pilings at one site, and PAH-contaminated sediments at Cordova Harbor. Our results indicate that PWS is remarkably free of pollution from PAH when nearby sources are absent as well as from pesticides and PCBs generally. PMID:18845332

  15. Spacer conformation in biologically active molecules. Part 2. Structure and conformation of 4-[2-(diphenylmethylamino)ethyl]-1-(2-methoxyphenyl) piperazine and its diphenylmethoxy analog—potential 5-HT 1A receptor ligands

    NASA Astrophysics Data System (ADS)

    Karolak-Wojciechowska, J.; Fruziński, A.; Czylkowski, R.; Paluchowska, M. H.; Mokrosz, M. J.

    2003-09-01

    As a part of studies on biologically active molecule structures with aliphatic linking chain, the structures of 4-[2-diphenylmethylamino)ethyl]-1-(2-methoxyphenyl)piperazine dihydrochloride ( 1) and 4-[2-diphenylmethoxy)ethyl]-1-(2-methoxyphenyl)piperazine fumarate ( 2) have been reported. In both compounds, four atomic non-all-carbons linking chains (N)C-C-X-C are present. The conformation of that linking spacer depends on the nature of the X-atom. The preferred conformation for chain with XNH has been found to be fully extended while for that with XO—the bend one. It was confirmed by conformational calculations (strain energy distribution and random search) and crystallographic data, including statistics from CCDC.

  16. 'They first killed his heart (then) he took his own life'. Part 1: a review of the context and literature on mental health issues for refugees and asylum seekers.

    PubMed

    Procter, Nicholas G

    2005-12-01

    This is the first in a two-part series of papers examining mental health issues for refugees and asylum seekers. Beginning with the suicide of an asylum seeker in Scotland, the paper emphasizes mental health issues for adult and child asylum seekers, stress and memory, suicide, self-harm, risk and protective factors, compulsory health treatments and the prevention of mental illness. It sets the scene for the second paper by drawing implications for nursing practice in the community. Although most literature on refugee and asylum seeker mental health exists outside of nursing scholarship, a majority of the issues reviewed in this paper are mutual challenges for all in the health and helping professions. Nurses interested in refugee and migration issues face two intertwined challenges: that of how to assist migrants with their diverse mental health needs and how, at the same time, to contribute to a society that can promote mental health for all by taking on both the difficulties and opportunities posed by cultural diversity.

  17. MIL1A

    Atmospheric Science Data Center

    2014-09-03

    ... MISR Level 1A camera charge-coupled device (CCD) Science Data: Reformatted Annotated Level 1A product of the CCD science data. ... Files:  Processing Status Production Report Read Software Files :  Data Product ...

  18. Apollo Mission Techniques Lunar Orbit Activities - Part 1a

    NASA Technical Reports Server (NTRS)

    Interbartolo, Michael A.

    2009-01-01

    This slide presentation reviews the planned sequence of events and the rationale for all lunar missions, and the flight experiences and lessons learned for the lunar orbit activities from a trajectory perspective. Shown are trajectories which include the moon's position at the various stages in the complete trip from launch, to the return and reentry. Included in the presentation are objectives and the sequence of events,for the Apollo 8, and Apollo 10. This is followed by a discussion of Apollo 11, including: the primary mission objective, the sequence of events, and the flight experience. The next mission discussed was Apollo 12. It reviews the objectives, the ground tracking, procedure changes, and the sequence of events. The aborted Apollo 13 mission is reviewed, including the objectives, and the sequence of events. Brief summaries of the flight experiences for Apollo 14-16 are reviewed. The flight sequence of events of Apollo 17 are discussed. In summary each mission consistently performing precision landings required that Apollo lunar orbit activities devote considerable attention to: (1) Improving fidelity of lunar gravity models, (2) Maximizing availability of ground tracking, (3) Minimizing perturbations on the trajectory, (4) Maximizing LM propellant reserves for hover time. Also the use of radial separation maneuvers (1) allows passive re-rendezvous after each rev, but ... (2) sensitive to small dispersions in initial sep direction

  19. U1A Complex

    ScienceCinema

    None

    2016-07-12

    Some of the most sophisticated experiments in the stockpile stewardship program are conducted in an environmentally safe manner, nearly 1000 feet below the ground at the site. The U1a complex a sprawling underground laboratory and tunnel complex is home to a number of unique capabilities.

  20. U1A Complex

    SciTech Connect

    2014-10-28

    Some of the most sophisticated experiments in the stockpile stewardship program are conducted in an environmentally safe manner, nearly 1000 feet below the ground at the site. The U1a complex a sprawling underground laboratory and tunnel complex is home to a number of unique capabilities.

  1. Motorcycle Parts

    NASA Technical Reports Server (NTRS)

    1993-01-01

    An article in NASA Tech Briefs describing a vacuum bagging process for forming composite parts helped a small Oklahoma Company to improve its manufacturing process. President of Performance Extremes, Larry Ortega, and his partners make motorcycle parts from carbon/epoxy to reduce weight. Using vacuum bags, parts have a better surface and fewer voids inside. When heat used in the vacuum bag process caused deformation upon cooling, a solution found in another tech brief solved the problem. A metal plate inside the vacuum bag made for more even heat transfer. A third article described a simple procedure for repairing loose connector pins, which the company has also utilized.

  2. Coronal partings

    NASA Astrophysics Data System (ADS)

    Nikulin, Igor F.; Dumin, Yurii V.

    2016-02-01

    The basic observational properties of "coronal partings"-the special type of quasi-one-dimensional magnetic structures, identified by a comparison of the coronal X-ray and EUV images with solar magnetograms-are investigated. They represent the channels of opposite polarity inside the unipolar large-scale magnetic fields, formed by the rows of magnetic arcs directed to the neighboring sources of the background polarity. The most important characteristics of the partings are discussed. It can be naturally assumed that-from the evolutionary and spatial points of view-the partings can transform into the coronal holes and visa versa. The classes of global, intersecting, and complex partings are identified.

  3. Bases para la elaboracion de unidades didacticas de calidad en el area de ciencias (Fisica y Quimica 3 deg ESO)

    NASA Astrophysics Data System (ADS)

    Maccioni, Elena Lucia

    Este Trabajo Fin de Master tiene como objeto, el estudio previo de la educacion de la ciencia en la actualidad y mas destacable, del diseno de las unidades didacticas segun las metodologias mas frecuentes aplicadas por los diferentes sistemas educativos en el area de las Ciencias teniendo en cuenta la importancia y el efecto de la psicologia del alumnado y todo ello como no puede ser de otro modo bajo el corse de la Legislacion aplicable, Estatal, Autonomica y europea. Con estos antecedentes, se extrae cuales son las preguntas que deben contestarse en la elaboracion de una unidad didactica de calidad en el contexto, generacional (edad y sexo), del Proyecto Educativo de Centro, y de la Programacion del Departamento, discutiendo y justificando cada uno de los apartados en que estructuradamente dividiremos dicha Unidad, con un formato manejable, util, y dinamico en el tiempo que sea un verdadero instrumento educativo de aula. Teniendo en cuenta estas premisas, se procede a hacer una recopilacion de una misma unidad didactica publicada por diferentes editoriales, elegidas no al azar, sino en funcion de su penetracion editorial en nuestros centros de ESO, siendo las elegidas (SM y Oxford). Las diferentes unidades seleccionadas son analizadas de forma critica, atendiendo a los criterios generales de calidad bajo parametros cientificos y normativos, concluyendo con la aportacion final que es la redaccion de unas pautas cientifico-pedagogicas, para redactar unidades didacticas de calidad en el area de la Ciencias, en concreto en la Asignatura de Fisica y Quimica de 3º de ESO.

  4. MISR Level 1A Products

    Atmospheric Science Data Center

    2013-04-01

    ... MISR Level 1A Products Level 1A Engineering Data File Type 1 and Level 1A Navigation Data Processing ... Product Specification Rev K  (PDF). Transparent software rebuild with Irix 6.5.2 OS. F01_0007 (FM_ENG), ...

  5. Peginterferon Beta-1a Injection

    MedlinePlus

    ... course of disease where symptoms flare up from time to time) of multiple sclerosis (MS, a disease in which ... peginterferon beta-1a injection at around the same time of day each time you inject it. Follow ...

  6. X-1A on lakebed

    NASA Technical Reports Server (NTRS)

    1955-01-01

    The Bell Aircraft Corporation X-1A (48-1384) is photographed in July 1955 sitting on Rogers Dry Lake at Edwards Air Force Base, California. This view of the left side of the aircraft shows the change to the X-1A canopy from the X-1s (see photo E49-0039 under XS-1) The nose boom carries an angle-of-attack and angle-of-sideslip vane, along with a pitot tube for measuring static and impact pressures. The fuselage length is 35 feet 8 inches, with a wing span of 28 feet. The X-1A was created to explore stability and control characteristics at speeds in excess of Mach 2 and altitudes greater than 90,000 feet. Bell test pilot Jean 'Skip' Ziegler made six test flights in the X-1A between 14 February and 25 April 1953. Air Force test pilots Maj. Charles 'Chuck' Yeager and Maj. Arthur 'Kit' Murray made 18 flights between 21 November 1953 and 26 August 1954. NACA test pilot Joseph Walker made one successful flight on 20 July 1955. During a second flight attempt, on 8 August 1955, an explosion damaged the X-1A shortly before launch. Walker, unhurt, climbed up into the JTB-29A mothership, and the X-1A was jettisoned over the Edwards AFB bombing range.

  7. Developments in Aging: 1976. Part 1. A Report of the Special Committee on Aging, United States Senate, Pursuant to S. Res. 373, March 1, 1976 Resolution Authorizing a Study of the Problems of the Aged and Aging Together with Minority Views. 95th Congress, 1st Session. Report No. 95-88.

    ERIC Educational Resources Information Center

    1977

    The first part of a report by the Special Committee on Aging of the United States Senate provides additional evidence about the need for far-ranging federal attention to the needs and aspirations of older Americans. A number of difficulties such as special transportation needs in rural areas, contradictions in policies and programs, and disaster…

  8. Steroids Update, Part 1 and Part 2.

    ERIC Educational Resources Information Center

    Miller, Calvin; Duda, Marty

    1986-01-01

    Part 1 of this two-part article describes the views of a physician who believes that athletes who want to take steroids are best protected by receiving a prescription and monitoring. Part 2 discusses the more general view of physicians that steroids should not be prescribed but perhaps should be monitored. (MT)

  9. Flowability of JSC-1a

    NASA Technical Reports Server (NTRS)

    Rame, Enrique; Wilkinson, Allen; Elliot, Alan; Young, Carolyn

    2009-01-01

    We have done a complete flowability characterization of the lunar soil simulant, JSC-1a, following closely the ASTM-6773 standard for the Schulze ring shear test. The measurements, which involve pre-shearing the material before each yield point, show JSC-1a to be cohesionless, with an angle of internal friction near 40 deg. We also measured yield loci after consolidating the material in a vibration table which show it to have significant cohesion (approximately equal to 1 kPa) and an angle of internal friction of about 60 deg. Hopper designs based on each type of flowability test differ significantly. These differences highlight the need to discern the condition of the lunar soil in the specific process where flowability is an issue. We close with a list not necessarily comprehensive of engineering rules of thumb that apply to powder flow in hoppers.

  10. Parts application handbook study

    NASA Technical Reports Server (NTRS)

    1978-01-01

    The requirements for a NASA application handbook for standard electronic parts are determined and defined. This study concentrated on identifying in detail the type of information that designers and parts engineers need and expect in a parts application handbook for the effective application of standard parts on NASA projects.

  11. Outline of the microgravity experiment rocket (TR-1A)

    NASA Astrophysics Data System (ADS)

    Kochiyama, Jiro

    1990-06-01

    In 1989, the National Space Development Agency of Japan (NASDA) decided to conduct the space processing experiment project, using the new microgravity experiment rocket (TR-1A), for the effective utilization of the Japanese Experiment Module, one part of the international space station. The project goal is to obtain the basic know-how of microgravity experiments and experimental devices through the flight opportunity of TR-1A (Test Rocket-1A) rocket. This TR-1A rocket can project a 1500 kg gross payload to about 270 km altitude and remain in a free fall condition about six minutes or more. Obtained microgravity condition is less than 10(exp -9)G. The first flight will be held in 1991, and now the development of the rocket and experimental devices are ongoing. This report describes an outline of the microgravity experiment rocket (TR-1A) and its standing point and prospect in the test rocket family.

  12. 40 CFR Appendix A to Part 63 - Test Methods

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....2.1A clearly written test method, preferably in the format of 40 CFR part 60, appendix A Test... reference from other methods in appendix A to 40 CFR part 60. Therefore, to obtain reliable results,...

  13. Music performance anxiety--part 1. A review of its epidemiology.

    PubMed

    Brugués, Ariadna Ortiz

    2011-06-01

    Music performance anxiety (MPA) affects many individuals regardless of age, gender, experience, and hours of practice. In order to better understand the epidemiology of MPA, a review of the literature was done. Sixteen articles, meeting EBM criteria, were identified and analyzed. Children rarely suffer from MPA, while adolescents show symptoms similar to adults. Females are generally more affected than males. There is no relation between professional experience and performance anxiety. Great musicians such as Pau Casals and Enrico Caruso suffered from MPA. Nevertheless, students affected by MPA may decide not to become professional musicians because of their inability to cope with the devastating effects of performance anxiety. Solo performances showed higher MPA scores than ensemble performances. Despite these conclusions, long-term cohort studies with larger, homogeneous groups of subjects would be desirable, according to the evidence-based medicine criteria.

  14. Morphology of the scattering targets: Fresnel and turbulent mechanisms, part 2.1A

    NASA Technical Reports Server (NTRS)

    Royrvik, O.

    1984-01-01

    Refractive index fluctuations cause coherent scattering and reflection of VHF radio waves from the clear air in the altitude region between 0 and approximately 90 km. Similar echoes from the stratosphere/troposphere and the mesosphere are observed at UHF and MF/HF frequencies, respectively. The nature of the refractive index fluctuations has been studied for many years without producing a clear consensus on what mechanism causes them. It is believed that the irregularities can originate from two different mechanisms: turbulent mixing of the gradient of refractive index, and stable horizontally stratified laminae of sharp gradients in the refractive index. In order to explain observations of volume dependence and aspect sensitivity of the echo power in the MST region, a diversity of submechanisms has been proposed. They include isotropic and anisotropic turbulent scattering, Fresnel scattering and reflection, and diffuse reflection. Isotropic turbulent scattering is believed to cause a majority of the clear air echoes observed by MST radars. The mechanism requires active turbulence mixing of a preexisting gradient in the refractive index profile.

  15. Is Income Inequality a Determinant of Population Health? Part 1. A Systematic Review

    PubMed Central

    Lynch, John; Smith, George Davey; Harper, Sam; Hillemeier, Marianne; Ross, Nancy; Kaplan, George A; Wolfson, Michael

    2004-01-01

    This article reviews 98 aggregate and multilevel studies examining the associations between income inequality and health. Overall, there seems to be little support for the idea that income inequality is a major, generalizable determinant of population health differences within or between rich countries. Income inequality may, however, directly influence some health outcomes, such as homicide in some contexts. The strongest evidence for direct health effects is among states in the United States, but even that is somewhat mixed. Despite little support for a direct effect of income inequality on health per se, reducing income inequality by raising the incomes of the most disadvantaged will improve their health, help reduce health inequalities, and generally improve population health. PMID:15016244

  16. Is income inequality a determinant of population health? Part 1. A systematic review.

    PubMed

    Lynch, John; Smith, George Davey; Harper, Sam; Hillemeier, Marianne; Ross, Nancy; Kaplan, George A; Wolfson, Michael

    2004-01-01

    This article reviews 98 aggregate and multilevel studies examining the associations between income inequality and health. Overall, there seems to be little support for the idea that income inequality is a major, generalizable determinant of population health differences within or between rich countries. Income inequality may, however, directly influence some health outcomes, such as homicide in some contexts. The strongest evidence for direct health effects is among states in the United States, but even that is somewhat mixed. Despite little support for a direct effect of income inequality on health per se, reducing income inequality by raising the incomes of the most disadvantaged will improve their health, help reduce health inequalities, and generally improve population health.

  17. 50 CFR Table 1a to Part 679 - Delivery Condition* and Product Codes

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    .... Throat, or isthmus, slit to allow blood to drain 03 Bled fish destined for fish meal (includes offsite.... Head removed, belly slit, viscera and most of backbone removed; fillets attached 37 Cheeks. Muscles on sides of head (ancillary only) 17 Chins. Lower jaw (mandible), muscles, and flesh (ancillary only)...

  18. Twenty-fourth Lunar and Planetary Science Conference. Part 1: A-F

    SciTech Connect

    Not Available

    1993-01-01

    The topics covered include the following: petrology, petrography, meteoritic composition, planetary geology, atmospheric composition, astronomical spectroscopy, lunar geology, Mars (planet), Mars composition, Mars surface, volcanology, Mars volcanoes, Mars craters, lunar craters, mineralogy, mineral deposits, lithology, asteroids, impact melts, planetary composition, planetary atmospheres, planetary mapping, cosmic dust, photogeology, stratigraphy, lunar craters, lunar exploration, space exploration, geochronology, tectonics, atmospheric chemistry, astronomical models, and geochemistry. Separate abstracts have been prepared for articles from this report.

  19. T/R switch design for short-range measurements, part 6.1A

    NASA Technical Reports Server (NTRS)

    Yu, B.

    1984-01-01

    The positive intrinsic negative (PIN) diode switch which is designed to protect the receiver from burnout or damage on transmission and channel the echo signal to the receiver on reception is outlined. The receiver must be protected firmly. A schematic diagram of a transformer rectifier (TR-ATR) switch for the Urbana Radar is shown. The T/R switch consists of a half wavelength coaxial cavity with tuning condenser and PIN diodes. Two UM4300 PIN diodes were mounted between the inner and outer conductor. The dc biasing voltage required for the PIN diodes is supplied by a control circuit. On transmission, the PIN diodes are forward biased to about 0.5 amperes. On reception, about 10 volts reverse voltage is applied to the diodes, which produces an initial reverse current to speed the recovery time. The T/R switch characteristics are estimated and the result of testing at different peak transmitter powers from 410 kW to 1500 kW is shown.

  20. Porphyria Diagnostics – Part 1: A brief overview of the porphyrias

    PubMed Central

    Ramanujam, Vaithamanithi-Mudumbai Sadagopa; Anderson, Karl Elmo

    2015-01-01

    The porphyria diseases are a group of metabolic disorders caused by abnormal functioning of heme biosynthesis enzymes and characterized by the excessive accumulation and excretion of porphyrins and their precursors. Precisely which of these chemicals builds up depends on the type of porphyria. Porphyria is not a single disease but a group of nine disorders: acute intermittent porphyria (AIP), hereditary coproporphyria (HCP), variegate porphyria (VP), delta-aminolevelunic acid dehyratase deficiency porphyria (ADP), porphyria cutanea tarda (PCT), hepatoerythropoetic porphyria (HEP), congenital erythropoetic porphyria (CEP), erythropoetic protoporphyria (EPP), and X-linked protoporphyria (XLP). Each porphyria results from overproduction of heme precursors secondary to partial deficiency or, in XLP, increased activity of one of the enzymes of heme biosynthesis. Taken together, all forms of porphyria afflict fewer than 200,000 people in the United States. Based on European studies, the prevalence of the most common porphyria, PCT, is 1 in 10,000, the most common acute porphyria, AlP, is about 1 in 20,000, and the most common erythropoietic porphyria, EPP, is estimated at 1 in 50,000 to 75,000. CEP is extremely rare with prevalence estimates of 1 in 1,000,000 or less. Only 6 cases of ADP are documented. The current porphyria literature is very exhaustive and a brief overview of porphyria diseases is essential in order for the reader to better appreciate the relevance of this area of research prior to undertaking biochemical diagnostics procedures. This unit summarizes the current knowledge on the classification, clinical features, etiology, pathogenesis, and genetics of these porphyria diseases. PMID:26132003

  1. 50 CFR Table 1a to Part 679 - Delivery Condition* and Product Codes

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Fillets, deep-skin. Meat with skin, adjacent meat with silver lining, and ribs removed from sides of body... skin and ribs removed, from sides of body behind head and in front of tail 23 Fillets with ribs, no skin. Meat with ribs with skin removed, from sides of body behind head and in front of tail 22...

  2. 50 CFR Table 1a to Part 679 - Delivery Condition* and Product Codes

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Fillets, deep-skin. Meat with skin, adjacent meat with silver lining, and ribs removed from sides of body... skin and ribs removed, from sides of body behind head and in front of tail 23 Fillets with ribs, no skin. Meat with ribs with skin removed, from sides of body behind head and in front of tail 22...

  3. Porphyria Diagnostics-Part 1: A Brief Overview of the Porphyrias.

    PubMed

    Ramanujam, Vaithamanithi-Mudumbai Sadagopa; Anderson, Karl Elmo

    2015-07-01

    Porphyria diseases are a group of metabolic disorders caused by abnormal functioning of heme biosynthesis enzymes and characterized by excessive accumulation and excretion of porphyrins and their precursors. Precisely which of these chemicals builds up depends on the type of porphyria. Porphyria is not a single disease but a group of nine disorders: acute intermittent porphyria (AIP), hereditary coproporphyria (HCP), variegate porphyria (VP), δ-aminolevulinic acid dehydratase deficiency porphyria (ADP), porphyria cutanea tarda (PCT), hepatoerythropoietic porphyria (HEP), congenital erythropoietic porphyria (CEP), erythropoietic protoporphyria (EPP), and X-linked protoporphyria (XLP). Each porphyria results from overproduction of heme precursors secondary to partial deficiency or, in XLP, increased activity of one of the enzymes of heme biosynthesis. Taken together, all forms of porphyria afflict fewer than 200,000 people in the United States. Based on European studies, the most common porphyria, PCT, has a prevalence of 1 in 10,000, the most common acute porphyria, AlP, has a prevalence of ∼1 in 20,000, and the most common erythropoietic porphyria, EPP, is estimated at 1 in 50,000 to 75,000. CEP is extremely rare, with prevalence estimates of 1 in 1,000,000 or less. Only six cases of ADP are documented. The current porphyria literature is very exhaustive and a brief overview of porphyria diseases is essential in order for the reader to better appreciate the relevance of this area of research prior to undertaking biochemical diagnostics procedures. This unit summarizes the current knowledge on the classification, clinical features, etiology, pathogenesis, and genetics of porphyria diseases.

  4. Frequency and site selection criteria for MST radars, part 5.1A

    NASA Technical Reports Server (NTRS)

    Nastrom, G. D.

    1984-01-01

    The majority of mesosphere-stratosphere-troposphere (MST) and ST radars are located in or near mountainous terrain. When measuring horizontal velocities, the terrain is a small factor, but when measuring vertical velocities, the meteorological noise induced by rough terrain can severely limit the usefulness of the observations. When the variance of the vertical velocity is too large, it is not possible to suitably filter the data to detect the small synoptic-scale signal with reasonable statistical confidence. The variance of vertical velocity at all tropospheric levels is directly related to the low level wind speed during flow over rough terrain. It is suggested that the synoptic-scale vertical velocity can be measured by ST radars where the terrain is smooth. The large-scale vertical velocity cannot always be reliably determined from MST radar data when the underlying terrain is rough. The vertical velocity is potentially on of future radar site selections, taking into account the desired meteorological applications of the data and engineering design factors. If the synoptic-scale vertical velocity is a desired variable, the radar should not be located near mountains.

  5. Coding schemes for improving MST radar performance, part 7.1A

    NASA Technical Reports Server (NTRS)

    Bowhill, S. A.

    1984-01-01

    The performance of an mesosphere-stratosphere-troposphere (MST) radar can be characterized by its system sensitivity and its range resolution. The former enables Doppler velocities to be determined even in the presence of very weak structures; the latter permits study of the fine structure within a turbulent region. Coding of transmitted signals has as its aim an increase in the effective radar sensitivity or range resolution without an increase in the peak transmitted power. This is accomplished by spreading the power in the frequency domain, giving better range resolution, without reducing the pulse width. Two basic techniques are used to accomplish this frequency dispersion: (1) using a type of pseudorandom code for the phase or amplitude within a single pulse, or within a finite sequence of pulses; (2) to code the frequency of the transmitted signal in some way. The various possibilities are discussed and are compared with the pulse-coding methods.

  6. 19 CFR Appendix to Part 163 - Interim (a)(1)(A) List

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... § 142.4* Bond information §§ 141.61, 142.3a* Broker/Importer Filer Number §§ 141.61, 142.3* Ultimate...: § 141.61* Entry Summary Date § 141.61* Entry Date § 142.3* Bond Number, Bond Type Code and Surety code... Country and Date Exported * I.T. (In-bond) Entry Date (for IT Entries only) * Mode of Transportation...

  7. 19 CFR Appendix to Part 163 - Interim (a)(1)(A) List

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... § 142.4* Bond information §§ 141.61, 142.3a* Broker/Importer Filer Number §§ 141.61, 142.3* Ultimate...: § 141.61* Entry Summary Date § 141.61* Entry Date § 142.3* Bond Number, Bond Type Code and Surety code... Country and Date Exported * I.T. (In-bond) Entry Date (for IT Entries only) * Mode of Transportation...

  8. 19 CFR Appendix to Part 163 - Interim (a)(1)(A) List

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... § 142.4* Bond information §§ 141.61, 142.3a* Broker/Importer Filer Number §§ 141.61, 142.3* Ultimate...: § 141.61* Entry Summary Date § 141.61* Entry Date § 142.3* Bond Number, Bond Type Code and Surety code... Country and Date Exported * I.T. (In-bond) Entry Date (for IT Entries only) * Mode of Transportation...

  9. Interactive dose shaping part 1: a new paradigm for IMRT treatment planning

    NASA Astrophysics Data System (ADS)

    Ziegenhein, Peter; Kamerling, Cornelis Ph; Oelfke, Uwe

    2016-03-01

    In this work we present a novel treatment planning technique called interactive dose shaping (IDS) to be employed for the optimization of intensity modulated radiation therapy (IMRT). IDS does not rely on a Newton-based optimization algorithm which is driven by an objective function formed of dose volume constraints on pre-segmented volumes of interest (VOIs). Our new planning technique allows for direct, interactive adaptation of localized planning features. This is realized by a dose modification and recovery (DMR) planning engine which implements a two-step approach: firstly, the desired localized plan adaptation is imposed on the current plan (modification) while secondly inevitable, undesired disturbances of the dose pattern elsewhere are compensated for automatically by the recovery module. Together with an ultra-fast dose update calculation method the DMR engine has been implemented in a newly designed 3D therapy planning system Dynaplan enabling true real-time interactive therapy planning. Here we present the underlying strategy and algorithms of the DMR based planning concept. The functionality of the IDS planning approach is demonstrated for a phantom geometry of clinical resolution and size.

  10. Game analytics for game user research, part 1: a workshop review and case study.

    PubMed

    El-Nasr, Magy Seif; Desurvire, Heather; Aghabeigi, Bardia; Drachen, Anders

    2013-01-01

    The emerging field of game user research (GUR) investigates interaction between players and games and the surrounding context of play. Game user researchers have explored methods from, for example, human-computer interaction, psychology, interaction design, media studies, and the social sciences. They've extended and modified these methods for different types of digital games, such as social games, casual games, and serious games. This article focuses on quantitative analytics of in-game behavioral user data and its emergent use by the GUR community. The article outlines open problems emerging from several GUR workshops. In addition, a case study of a current collaboration between researchers and a game company demonstrates game analytics' use and benefits.

  11. Transcontinental methane measurements: Part 1. A mobile surface platform for source investigations

    NASA Astrophysics Data System (ADS)

    Farrell, Paige; Culling, Daniel; Leifer, Ira

    2013-08-01

    The potent greenhouse gas, methane, CH4, originates from a wide range of anthropogenic and natural sources. A ground-based, satellite-scale, transcontinental (Florida to California) survey was conducted to understand better emissions from key sources including wetlands, forest fire, and geologic sources, as well as to acquire desert background values and lower atmosphere vertical profiling in the San Bernardino Mountains. A total of 6600 measurements along 7020 km of roadways were made by flame ion detection, gas chromatography (GC) onboard a recreational vehicle in 2010, and during a second survey with a cavity ring-down spectrometer system in Southern California in 2012. Significant vibration reduction efforts allowed near continuous mobile GC measurements. Nocturnal CH4 measurements tended to be higher compared to daytime values, sometime significantly, for similar sources and were concluded due to day/night meteorological differences. The lowest GC observations were 1.80 ppm, observed in the California desert, ˜60 ppb less than minimum desert CH4 observed in 2012. Thanks to smoke visualization of a brush fire plume, the flux from the fire was estimated at 0.15 kiloton day-1. Geologic CH4 emissions from the La Brea tar pit and surrounding areas were surprisingly strong, with peak concentrations of nearly 50 ppm and highly elevated CH4 concentrations extending over at least ˜100 km2, and accounting potentially for a significant fraction of the LA basin CH4 emissions. Geologic CO2 emissions also were observed.

  12. Patients' experiences of privacy and dignity. Part 1: a literature review.

    PubMed

    Whitehead, Juliet; Wheeler, Herman

    This article reviews the literature on patients' privacy and dignity and concludes that interest in the topic goes back to psychiatric patients in the 1960s, with the 1990s seeing a shift to all hospitals. A number of databases were used to obtain information for the literature review. Findings revealed United Kingdom (UK) government policies designed to raise the profile of patients' privacy and dignity. UK research on patients' privacy/dignity is limited, although there is universal interest. While a variety of methodologies have been used in previous research, and there is consistency in findings, more research is indicated. Previous research spanned topics such as elderly patients, medical and surgical patients, palliative care, rehabilitative settings and child-bearing women. A conceptual framework and definitions of privacy and dignity were examined. Although healthcare professionals and patients attach importance to patients' privacy, there is insufficient understanding of the problem. Limitations of previous research have been discussed.

  13. Bed bugs, public health, and social justice: Part 1, A call to action.

    PubMed

    Eddy, Christopher; Jones, Susan C

    2011-04-01

    The resurgence of bed bugs poses an urgent situation since infestations are rampant globally, nationally, and locally. In Ohio, bed bugs have become a virtual epidemic in many towns and cities, especially in central and southwestern regions of the state. These blood-feeding insects cause an array of adverse health effects in humans. Furthermore, bed bugs disproportionately occur in urban areas, and housing and the built environment are now recognized as dominant influences on health. Bed bugs' potential role in disease transmission remains unqualified to date, and research on this issue is urgently needed. The escalating global bed bug resurgence leaves the divided public health community in a precarious social justice position if the lack of response to bed bug infestations disproportionately impacts underserved populations. Bed bugs are an urgent public health and environmental justice concern, and the authors recommend that public health agencies respond with authority of agency.

  14. Epistemic uncertainties and natural hazard risk assessment - Part 1: A review of the issues

    NASA Astrophysics Data System (ADS)

    Beven, K. J.; Aspinall, W. P.; Bates, P. D.; Borgomeo, E.; Goda, K.; Hall, J. W.; Page, T.; Phillips, J. C.; Rougier, J. T.; Simpson, M.; Stephenson, D. B.; Smith, P. J.; Wagener, T.; Watson, M.

    2015-12-01

    Uncertainties in natural hazard risk assessment are generally dominated by the sources arising from lack of knowledge or understanding of the processes involved. There is a lack of knowledge about frequencies, process representations, parameters, present and future boundary conditions, consequences and impacts, and the meaning of observations in evaluating simulation models. These are the epistemic uncertainties that can be difficult to constrain, especially in terms of event or scenario probabilities, even as elicited probabilities rationalized on the basis of expert judgements. This paper reviews the issues raised by trying to quantify the effects of epistemic uncertainties. Such scientific uncertainties might have significant influence on decisions that are made for risk management, so it is important to communicate the meaning of an uncertainty estimate and to provide an audit trail of the assumptions on which it is based. Some suggestions for good practice in doing so are made.

  15. Twenty-fourth Lunar and Planetary Science Conference. Part 1: A-F

    NASA Technical Reports Server (NTRS)

    1993-01-01

    The topics covered include the following: petrology, petrography, meteoritic composition, planetary geology, atmospheric composition, astronomical spectroscopy, lunar geology, Mars (planet), Mars composition, Mars surface, volcanology, Mars volcanoes, Mars craters, lunar craters, mineralogy, mineral deposits, lithology, asteroids, impact melts, planetary composition, planetary atmospheres, planetary mapping, cosmic dust, photogeology, stratigraphy, lunar craters, lunar exploration, space exploration, geochronology, tectonics, atmospheric chemistry, astronomical models, and geochemistry.

  16. The Twenty-Fifth Lunar and Planetary Science Conference. Part 1: A-G

    NASA Technical Reports Server (NTRS)

    1994-01-01

    Papers from the conference are presented, and the topics covered include the following: planetary geology, meteorites, planetary composition, meteoritic composition, planetary craters, lunar craters, meteorite craters, petrology, petrography, volcanology, planetary crusts, geochronology, geomorphism, mineralogy, lithology, planetary atmospheres, impact melts, volcanoes, planetary evolution, tectonics, planetary mapping, asteroids, comets, lunar soil, lunar rocks, lunar geology, metamorphism, chemical composition, meteorite craters, and planetary mantles.

  17. Adult-Rated Oceanography Part 1: A Project Integrating Ocean Sciences into Adult Basic Education Programs.

    NASA Astrophysics Data System (ADS)

    Cowles, S.; Collier, R.; Torres, M. K.

    2004-12-01

    Busy scientists seek opportunities to implement education and outreach efforts, but often don't know where to start. One easy and tested method is to form collaborations with federally-funded adult education and adult literacy programs. These programs exist in every U.S. state and territory and serve underrepresented populations through such major initiatives as adult basic education, adult secondary education (and GED preparation), and English language acquisition. These students are workers, consumers, voters, parents, grandparents, and members of every community. They have specific needs that are often overlooked in outreach activities. This presentation will describe the steps by which the Oregon Ocean Science and Math Collaborative program was developed. It is based on a partnership between the Oregon Department of Community Colleges and Workforce Development, Oregon State University College of Oceanic and Atmospheric Sciences, Oregon Sea Grant, and the OSU Hatfield Marine Science Center. It includes professional development through instructor institutes; teachers at sea and informal education opportunities; curriculum and web site development. Through the partnership described here, instructors in adult basic education programs participate in a yearlong experience in which they develop, test, and adapt innovative instructional strategies to meet the specific needs of adult learners. This, in turn, leads to new prospects for study in the areas of ocean science and math and introduces non-academic careers in marine science to a new community. Working directly with instructors, we have identified expertise level, instructional environment, instructor background and current teaching strategies used to address science literacy and numeracy goals of the adult learners in the State of Oregon. Preliminary evaluation of our ongoing project in meeting these goals will be discussed. These efforts contribute to national goals of science literacy for all, by providing learning activities that link ocean sciences with real-life issues relevant to employment, environment and economic concerns.

  18. Canopy Spectral Invariants. Part 1: A New Concept in Remote Sensing of Vegetation

    NASA Technical Reports Server (NTRS)

    Knyazikhin, Yuri; Schull, Mitchell A.; Xu, Liang; Myneni, Ranga B.; Samanta, Arindam

    2011-01-01

    The concept of canopy spectral invariants expresses the observation that simple algebraic combinations of leaf and canopy spectral reflectance become wavelength independent and determine two canopy structure specific variables the recollision and escape probabilities. These variables specify an accurate relationship between the spectral response of a vegetation canopy to incident solar radiation at the leaf and the canopy scale. They are sensitive to important structural features of the canopy such as forest cover, tree density, leaf area index, crown geometry, forest type and stand age. This paper presents the mathematical basis of the concept which is linked to eigenvalues and eigenvectors of the three-dimensional radiative transfer equation.

  19. Ontology Research and Development. Part 1-A Review of Ontology Generation.

    ERIC Educational Resources Information Center

    Ding, Ying; Foo, Schubert

    2002-01-01

    Discusses the role of ontology in knowledge representation, including enabling content-based access, interoperability, communications, and new levels of service on the Semantic Web; reviews current ontology generation studies and projects as well as problems facing such research; and discusses ontology mapping, information extraction, natural…

  20. Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products.

    PubMed Central

    Harlow, E; Franza, B R; Schley, C

    1985-01-01

    Hybridomas secreting monoclonal antibodies specific for the adenovirus early region 1A (E1A) proteins were prepared from BALB/c mice immunized with a bacterial trpE-E1A fusion protein. This protein is encoded by a hybrid gene that joins a portion of the Escherichia coli trpE gene and a cDNA copy of the E1A 13S mRNA (Spindler et al., J. Virol. 49:132-141, 1984). Eighty-three hybridomas that secrete antibodies which recognize the immunogen were isolated and single cell cloned. Twenty-nine of these antibodies are specific for the E1A portion of the fusion protein. Only 12 of the monoclonal antibodies can efficiently immunoprecipitate E1A polypeptides from detergent lysates of infected cells. E1A polypeptides were analyzed on one-dimensional, sodium dodecyl sulfate-polyacrylamide gels and two-dimensional, isoelectric focusing polyacrylamide gels. The E1A proteins that are specifically immunoprecipitated by the monoclonal antibodies are heterogeneous in size and charge and can be resolved into approximately 60 polypeptide species. This heterogeneity is due not only to synthesis from multiple E1A mRNAs, but also at least in part to post-translational modification. Several of the monoclonal antibodies divide the E1A polypeptides into immunological subclasses based on the ability of the antibodies to bind to the antigen. In particular, two of the monoclonal antibodies bind to the polypeptides synthesized from the 13S E1A mRNA, but not to other E1A proteins. Images PMID:3894685

  1. Microlabels For Auto Parts

    NASA Technical Reports Server (NTRS)

    Ash, John P.

    1993-01-01

    Proposed method of unique labeling and identification of automotive parts greatly simplifies recall campaigns and reduces effort and expense associated. Compressed symbols fully characterize each part by type and manufacturing history. Manufacturers notify only those owners whose cars need repairs or modifications. Similar compressed symbology developed for possible use on spacecraft.

  2. Dissecting Diversity Part II

    ERIC Educational Resources Information Center

    Matthews, Frank

    2005-01-01

    This article presents "Dissecting Diversity, Part II," the conclusion of a wide-ranging two-part roundtable discussion on diversity in higher education. The participants were as follows: Lezli Baskerville, J.D., President and CEO of the National Association for Equal Opportunity (NAFEO); Dr. Gerald E. Gipp, Executive Director of the American…

  3. 9 CFR 73.1a - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false 73.1a Section 73.1a Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS SCABIES IN CATTLE § 73.1a...

  4. 9 CFR 73.1a - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false 73.1a Section 73.1a Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS SCABIES IN CATTLE § 73.1a...

  5. 9 CFR 73.1a - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false 73.1a Section 73.1a Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS SCABIES IN CATTLE § 73.1a...

  6. 9 CFR 73.1a - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false 73.1a Section 73.1a Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS SCABIES IN CATTLE § 73.1a...

  7. 9 CFR 73.1a - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false 73.1a Section 73.1a Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE INTERSTATE TRANSPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS SCABIES IN CATTLE § 73.1a...

  8. Constitutive relations in TRAC-P1A

    SciTech Connect

    Rohatgi, U.S.; Saha, P.

    1980-08-01

    The purpose of this document is to describe the basic thermal-hydraulic models and correlations that are in the TRAC-P1A code, as released in March 1979. It is divided into two parts, A and B. Part A describes the models in the three-dimensional vessel module of TRAC, whereas Part B focuses on the loop components that are treated by one-dimensional formulations. The report follows the format of the questions prepared by the Analysis Development Branch of USNRC and the questionnaire has been attached to this document for completeness. Concerted efforts have been made in understanding the present models in TRAC-P1A by going through the FORTRAN listing of the code. Some discrepancies between the code and the TRAC-P1A manual have been found. These are pointed out in this document. Efforts have also been made to check the TRAC references for the range of applicability of the models and correlations used in the code. 26 refs., 5 figs., 1 tab.

  9. Pieces and Parts

    ERIC Educational Resources Information Center

    Miller, Deborah A.; Ellis, Edith B.

    2006-01-01

    Although critical to understanding human sexuality and reproductive physiology, the male and female reproductive anatomy is often minimally covered by teachers. For teachers and students alike, it is often an embarrassing topic to discuss. This activity allows students and teachers to identify the parts of both reproductive systems with little…

  10. Part-Time Employment.

    ERIC Educational Resources Information Center

    Guichard, Gus; And Others

    The employment of community college instructors on a part-time basis provides the opportunity for students to study under outstanding instructors whose primary employment may be in industry or in other postsecondary institutions and permits colleges to respond better to community needs with the financial resources available to them. Along with…

  11. Basic Electricity. Part 3.

    ERIC Educational Resources Information Center

    Kilmer, Donald C.

    Third (part 3) in a set of four guides designed for the student interested in a vocation in electrical work, this guide includes four units: Unit VI--Ohm's Law, covering six lessons (voltage, current-flow and resistance, the Ohm's Law formula, formula for finding voltage, formula for finding resistance); Unit VII--Voltages, covering five lessons…

  12. Basic Electricity. Part 2.

    ERIC Educational Resources Information Center

    Kilmer, Donald C.

    This guide, the second (part 2) in a set of four guides, is designed for the student interested in a vocation in electrical work, and includes two units: Unit IV--Electrical Theory, covering thirteen lessons (matter, the atom, electrical charges in the atom, rules of electric charges, electricity, atoms in an electrical conductor, electrical…

  13. Meatcutting Testbook, Part 2.

    ERIC Educational Resources Information Center

    California State Dept. of Education, Sacramento. Bureau of Publications.

    This document contains objective tests for each topic in the Meatcutting Workbook, Part 2, which is designed for apprenticeship meatcutting programs in California. Each of the 30 tests consists of from 5 to 65 multiple-choice items with most tests containing approximately 10 items. The tests are grouped according to the eight units of the…

  14. Rockets -- Part II.

    ERIC Educational Resources Information Center

    Leitner, Alfred

    1982-01-01

    If two rockets are identical except that one engine burns in one-tenth the time of the other (total impulse and initial fuel mass of the two engines being the same), which rocket will rise higher? Why? The answer to this question (part 1 response in v20 n6, p410, Sep 1982) is provided. (Author/JN)

  15. Meatcutting Testbook, Part I.

    ERIC Educational Resources Information Center

    Strazicich, Mirko, Ed.

    This document contains objective tests for each lesson in the Meatcutting Workbook, Part I (see note), which is designed for apprenticeship programs in meatcutting in California. Each of the 36 tests contains from 10 to 45 multiple-choice items. The tests are grouped according to the eight units of the workbook: the apprentice meatcutter; applied…

  16. Understanding Math - Part I.

    ERIC Educational Resources Information Center

    Wyks, Hollis W.; Austin, Robert J.

    This is the first workbook-text in a two-part series written for deaf students. It is remedial in nature, aimed at the secondary level, and covers addition, subtraction, multiplication, and division of whole numbers. The use of the number 10 in explaining the concepts presented is stressed throughout. For the second workbook, see SE 015 828, and…

  17. Understanding Math - Part II.

    ERIC Educational Resources Information Center

    Wyks, Hollis W.; Austin, Robert J.

    This is the second remedial workbook-text in a two-part series written for deaf students at the secondary level. It covers fractions, geometry formulas, decimals and percents, and time. For the first workbook, see SE 015 827, and for the teacher's guide, see SE 015 829. (DT)

  18. Distinción Empírica Entre Engagement y Trabajolismo en Enfermeras Hospitalarias de Japón: Efecto Sobre la Calidad del Sueño y el Desempeño Laboral

    PubMed Central

    Kubota, Kazumi; Shimazu, Akihito; Kawakami, Norito; Takahashi, Masaya; Nakata, Akinori; Schaufeli, Wilmar B.

    2016-01-01

    Objetivo El objetivo de este estudio es demostrar la distinción entre engagement y trabajolismo, estudiando su relación con la calidad del sueño y el desempeño laboral. Método Un total de 447 enfermeras de 3 hospitales de Japón fueron entrevistadas mediante un cuestionario autoadministrado que incluía la escala Utrecht (UWES, Utrecht Work Engagement Scale), la Escala de Adicción al Trabajo Holandesa (DUWAS, Dutch Workaholism Scale), preguntas sobre la calidad del sueño (7 ítems) con respecto a (1) dificultad para conciliar el sueño, (2) dificultad para mantener el sueño, (3) despertar temprano por la mañana, (4) dormirse o tomar siestas durante el día, (5) somnolencia diurna excesiva en el trabajo, (6) dificultad para despertarse por la mañana, y (7) despertar cansado en la mañana, y el Cuestionario sobre Salud y Desempeño (CSD) de la Organización Mundial de la Salud. Resultados Los modelos de ecuaciones estructurales demostraron que el engagement se relaciona positivamente con la calidad del sueño y el rendimiento laboral, mientras que el trabajolismo tiene una relación negativa con la calidad del sueño y el desempeño laboral. Conclusión Los resultados indican que el engagement y el trabajolismo son conceptualmente diferentes. El primero tiene una connotación positiva, mientras que el segundo se asocia de manera negativa al bienestar (buena calidad del sueño y buen rendimiento en el trabajo). PMID:26752805

  19. Concrete samples for organic samples, data package and 222-S validation summary report. Addendum 1A

    SciTech Connect

    Vogel, R.E.

    1994-11-01

    This document is in two parts: the first is the data package entitled ``Concrete Samples for Organic Samples`` and the second is entitled ``Concrete Samples for Organic Samples -- Addendum 1A`` which is the 222-S validation summary report.

  20. Psychometric performance of the brazilian version of the Mini-cuestionario de calidad de vida en la hipertensión arterial (MINICHAL).

    PubMed

    Soutello, Ana Lúcia Soares; Rodrigues, Roberta Cunha Matheus; Jannuzzi, Fernanda Freire; Spana, Thaís Moreira; Gallani, Maria Cecília Bueno Jayme; Nadruz Junior, Wilson

    2011-01-01

    This study aimed to evaluate the feasibility, acceptability, ceiling and floor effects, reliability, and convergent construct validity of the Brazilian version of the Mini Cuestionario de Calidad de Vida en la Hipertensión Arterial (MINICHAL). The study included 200 hypertensive outpatients in a university hospital and a primary healthcare unit. The MINICHAL was applied in 3.0 (± 1.0) minutes with 100% of the items answered. A "ceiling effect" was observed in both dimensions and in the total score, as well as evidence of measurement stability (ICC=0.74). The convergent validity was confirmed by significant positive correlations between similar dimensions of the MINICHAL and the SF-36, and significant negative correlations with the Minnesota Living with Heart Failure Questionnaire - MLHFQ, however, correlations between dissimilar constructs were also observed. It was concluded that the Brazilian version of the MINICHAL presents evidence of reliability and validity when applied to hypertensive outpatients.

  1. Interferon Beta-1a Intramuscular Injection

    MedlinePlus

    ... course of disease where symptoms flare up from time to time) of multiple sclerosis (MS, a disease in which ... interferon beta-1a intramuscular at around the same time of day on your injection days. Follow the ...

  2. The phenotype range of achondrogenesis 1A.

    PubMed

    Grigelioniene, Giedre; Geiberger, Stefan; Papadogiannakis, Nikos; Mäkitie, Outi; Nishimura, Gen; Nordgren, Ann; Conner, Peter

    2013-10-01

    Achondrogenesis 1A (ACG1A; OMIM 200600) is an autosomal recessive perinatally lethal skeletal dysplasia comprising intrauterine growth failure, micromelia, minor facial anomalies, deficient ossification of the skull, absent or extremely defective spinal ossification, short beaded ribs, and short deformed long bones with a stellate appearance. ACG1A is caused by mutations in the TRIP11 gene, resulting in deficiency of the Golgi microtubule associated protein 210. In this study we describe dizygotic twins with a clinical and radiological phenotype of ACG1A who were homozygous for a novel nonsense mutation in the TRIP11 gene. In addition, another patient with a milder manifestation, not readily distinguishable from those of other lethal skeletal dysplasias, was found to be a compound heterozygote for a nonsense mutation and a deletion of the 3' end of the TRIP11 gene. We conclude that mutations of the TRIP11 gene may encompass a wider phenotypic range than previously recognized. PMID:23956106

  3. Just Perfect, Part 2

    ERIC Educational Resources Information Center

    Scott, Paul

    2007-01-01

    In "Just Perfect: Part 1," the author defined a perfect number N to be one for which the sum of the divisors d (1 less than or equal to d less than N) is N. He gave the first few perfect numbers, starting with those known by the early Greeks. In this article, the author provides an extended list of perfect numbers, with some comments about their…

  4. H5Part

    2005-12-01

    Particle based simulations of accelerator beam-lines, especially in six dimensional phase space, generate vast amounts of data. Even though a subset of statistical information regarding phase space or analysis needs to be preserved, reading and writing such enormous restart files on massively parallel supercomputing systems remains challenging. H5Part is a very simple data storage schema and provides an API that simplifies the reading/writing of the data to the HDF5 file format. An important foundation formore » a stable visualization and data analysis environment is a stable and portable file storage format and its associated APIs. The presence of a "common file storage format," including associated APIs, will help foster a fundamental level of interoperability across the project’s software infrastructure. It will also haip ensure that key data analysis capabilities are present during the earliest phases of the software development effort. H5Part is built on top of the HDF5 (Hierarchical Data Format). HDF5 offers a self.describing machine-independent binary file format that supports scalable parallel I/O performance for MPI codes on a variety of supercomputlng systems, and works equally well on laptop computers. The API Is available (or C, C++, and Fortran codes. The H5Part file format and APIs enable disparate research groups with different simulation implementations to transparently share datasets and data analysis tools. For instance, the common file format will enable groups that depend on completely different simulation implementations to share data analysis tools like PartView and AVS/Express without any additional programming.« less

  5. Ras Regulates Rb via NORE1A.

    PubMed

    Barnoud, Thibaut; Donninger, Howard; Clark, Geoffrey J

    2016-02-01

    Mutations in the Ras oncogene are one of the most frequent events in human cancer. Although Ras regulates numerous growth-promoting pathways to drive transformation, it can paradoxically promote an irreversible cell cycle arrest known as oncogene-induced senescence. Although senescence has clearly been implicated as a major defense mechanism against tumorigenesis, the mechanisms by which Ras can promote such a senescent phenotype remain poorly defined. We have shown recently that the Ras death effector NORE1A plays a critical role in promoting Ras-induced senescence and connects Ras to the regulation of the p53 tumor suppressor. We now show that NORE1A also connects Ras to the regulation of a second major prosenescent tumor suppressor, the retinoblastoma (Rb) protein. We show that Ras induces the formation of a complex between NORE1A and the phosphatase PP1A, promoting the activation of the Rb tumor suppressor by dephosphorylation. Furthermore, suppression of Rb reduces NORE1A senescence activity. These results, together with our previous findings, suggest that NORE1A acts as a critical tumor suppressor node, linking Ras to both the p53 and the Rb pathways to drive senescence.

  6. Immunomodulation. Part IV: Glutamine.

    PubMed

    Bell, Susan Givens

    2006-01-01

    Glutamine, a nonessential amino acid that appears to be conditionally essential during periods of physiologic stress, plays important physiologic roles in the immune system. However, neither enteral nor parenteral glutamine supplementation makes a difference in the rate of systemic infection or of NEC in very low birth weight infants. Thus, the search for agents to enhance the neonate's immune system and to serve as safe and effective adjuvants to antibiotics continues. Part V, the final article in this immunomodulation series, will explore the use of probiotics to support the neonatal immune system.

  7. Endocrine system: part 1.

    PubMed

    Johnstone, Carolyn; Hendry, Charles; Farley, Alistair; McLafferty, Ella

    2014-05-27

    This article, which forms part of the life sciences series and is the first of two articles on the endocrine system, examines the structure and function of the organs of the endocrine system. It is important that nurses understand how the endocrine system works and its role in maintaining health. The role of the endocrine system and the types, actions and control of hormones are explored. The gross structure of the pituitary and thyroid glands are described along with relevant physiology. Several disorders of the thyroid gland are outlined. The second article examines growth hormone, the pancreas and adrenal glands.

  8. 78 FR 69539 - Removal of Attestation Process for Facilities Using H-1A Registered Nurses

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-20

    ...=FR . SUPPLEMENTARY INFORMATION: In 1989, Congress created an H-1A nonimmigrant classification... implemented the H-1A program through regulations at 20 CFR part 655 Subparts D and E. See 55 FR 50500 (Dec. 6, 1990), as amended by 59 FR 874 (Jan. 6, 1994). Because of the expiration of the authorizing...

  9. UDP-glucuronosyltransferases 1A6 and 1A10 catalyze reduced menadione glucuronidation

    SciTech Connect

    Nishiyama, Takahito; Ohnuma, Tomokazu; Inoue, Yuu; Kishi, Takehiko; Ogura, Kenichiro; Hiratsuka, Akira

    2008-06-27

    Menadione (2-methyl-1,4-naphthoquine), also known as vitamin K3, has been widely used as a model compound in the field of oxidative stress-related research. The metabolism of menadione has been studied, and it is known that menadione undergoes a two-electron reduction by NAD(P)H:Quinone oxidoreductase 1 (NQO1) after which the reduced form of menadione (2-methyl-1,4-naphthalenediol, menadiol) is glucuronidated and excreted in urine. To investigate which human UDP-glucuronosyltransferase (UGT) isoforms participate in the glucuronidation of menadiol reduced by NQO1 from menadione, we first constructed heterologously expressed NQO1 in Sf9 cells and tested the menadiol glucuronidating activity of 16 human recombinant UGT isoforms. Of the 16 UGT isoforms, UGTs 1A6, 1A7, 1A8, 1A9, and 1A10 catalyzed menadiol glucuronidation, and, of these, UGTs 1A6 and 1A10 catalyzed menadiol glucuronidation at much higher rates than the other UGTs. Menadiol was regioselectively glucuronidated in the manner of 4-position > 1-position by UGTs 1A7, 1A8, 1A9, and 1A10. In contrast to these UGTs, only UGT1A6 exhibited 1-menadiol-preferential glucuronidating activity. The results suggest possible detoxification pathways for quinones via NQO1 reduction followed by UGT glucuronidation.

  10. 16 CFR Figure 2 to Part 1511 - Small Parts Gage

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Small Parts Gage 2 Figure 2 to Part 1511 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR PACIFIERS Pt.1511, Fig. 2 Figure 2 to Part 1511—Small Parts Gage EC03OC91.069...

  11. 16 CFR Figure 2 to Part 1511 - Small Parts Gage

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Small Parts Gage 2 Figure 2 to Part 1511 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR PACIFIERS Pt.1511, Fig. 2 Figure 2 to Part 1511—Small Parts Gage EC03OC91.069...

  12. 16 CFR Figure 2 to Part 1511 - Small Parts Gage

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Small Parts Gage 2 Figure 2 to Part 1511 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR PACIFIERS Pt.1511, Fig. 2 Figure 2 to Part 1511—Small Parts Gage EC03OC91.069...

  13. 16 CFR Figure 2 to Part 1511 - Small Parts Gage

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Small Parts Gage 2 Figure 2 to Part 1511 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR PACIFIERS Pt.1511, Fig. 2 Figure 2 to Part 1511—Small Parts Gage EC03OC91.069...

  14. 16 CFR Figure 2 to Part 1511 - Small Parts Gage

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Small Parts Gage 2 Figure 2 to Part 1511 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION FEDERAL HAZARDOUS SUBSTANCES ACT REGULATIONS REQUIREMENTS FOR PACIFIERS Pt.1511, Fig. 2 Figure 2 to Part 1511—Small Parts Gage EC03OC91.069...

  15. Role of PPM1A and anti-PPM1A Autoantibodies in Ankylosing Spondylitis

    PubMed Central

    Zhao, Xiaoyan; Sokolove, Jeremy; Lindstrom, Tamsin M.; Yoo, Bin; Lee, Chang-Keun; Reveille, John D.; Taurog, Joel D.; Robinson, William H.

    2014-01-01

    Objective Although ankylosing spondylitis (AS) is driven by immunemediated processes, little is known about the presence and role of autoantibodies in this disease. Methods We performed human protein microarray analysis of sera derived from patients with AS and other autoimmune disorders to identify autoantibodies associated specifically with AS, and identified autoantibody targeting of protein phosphatase magnesium-dependent 1A (PPM1A) in AS. We performed ELISA analysis of sera from two independent AS cohorts to confirm autoantibody targeting of PPM1A, and to assess associations between levels of anti-PPM1A antibodies and AS disease severity or response (as measured by BASDAI score) to anti-TNF therapy. Levels of anti-PPM1A antibodies were also evaluated in sera from transgenic rats overexpressing HLA-B27 and human β2-microglobulin. The expression of PPM1A was assessed by immunohistochemistry in synovial tissues from patients with AS, rheumatoid arthritis, or osteoarthritis. The role of PPM1A on osteoblast differentiation was investigated by gene knock-down and overexpression. Results AS was associated with autoantibody targeting of PPM1A, and levels of anti-PPM1A autoantibodies were significantly higher in patients with more advanced sacroiliitis and correlated with BASDAI score after treatment with anti-TNF agents. The levels of anti-PPM1A autoantibodies were also higher in sera of transgenic rats that are prone to develop AS than in those that are not. PPM1A was expressed in AS synovial tissue, and PPM1A overexpression promoted osteoblast differentiation, whereas PPM1A knockdown suppressed it. Conclusions Anti-PPM1A autoantibodies are present in AS, and our findings suggest that PPM1A may contribute to the pathogenic bone ankylosis characteristic of AS. PMID:24980965

  16. Smoothed particle hydrodynamics with GRAPE-1A

    NASA Technical Reports Server (NTRS)

    Umemura, Masayuki; Fukushige, Toshiyuki; Makino, Junichiro; Ebisuzaki, Toshikazu; Sugimoto, Daiichiro; Turner, Edwin L.; Loeb, Abraham

    1993-01-01

    We describe the implementation of a smoothed particle hydrodynamics (SPH) scheme using GRAPE-1A, a special-purpose processor used for gravitational N-body simulations. The GRAPE-1A calculates the gravitational force exerted on a particle from all other particles in a system, while simultaneously making a list of the nearest neighbors of the particle. It is found that GRAPE-1A accelerates SPH calculations by direct summation by about two orders of magnitudes for a ten thousand-particle simulation. The effective speed is 80 Mflops, which is about 30 percent of the peak speed of GRAPE-1A. Also, in order to investigate the accuracy of GRAPE-SPH, some test simulations were executed. We found that the force and position errors are smaller than those due to representing a fluid by a finite number of particles. The total energy and momentum were conserved within 0.2-0.4 percent and 2-5 x 10 exp -5, respectively, in simulations with several thousand particles. We conclude that GRAPE-SPH is quite effective and sufficiently accurate for self-gravitating hydrodynamics.

  17. Electronic communication. Part III.

    PubMed

    Bergren, M D

    1995-02-01

    This is the concluding article of a three-part series on electronic communication for school nurses. The October 1994 column described electronic communication and the hardware and software required. The December 1994 column examined e-mail, bulletin boards, databases, and file transfers. This column will list many health and nursing resources available on-line. Some of the resources are available only through the Internet. Others are accessible by more than one route: dial-in, telnet, gopher, or world wide web. A few of the services, such as MEDLINE, are only accessed with purchased accounts (Glowniak & Bushway, 1994). The electronic resources of interest to school nurses are so numerous it would be impossible to cite all of them in a column of this length. Selected resources for the school health provider will be listed in alphabetical order. PMID:7767047

  18. Rickets: Part I.

    PubMed

    Shore, Richard M; Chesney, Russell W

    2013-01-01

    Rickets is characterized by impaired mineralization and ossification of the growth plates of growing children caused by a variety of disorders, the most frequent of which is nutritional deficiency of vitamin D. Despite ample knowledge of its etiology and the availability of cost-effective methods of preventing it, vitamin D deficiency rickets remains a significant problem in developing and developed countries. This two-part review covers the history, etiology, pathophysiology and clinical and radiographical findings of vitamin D deficiency rickets. Other less frequent causes of rickets and some of the disorders entering into the differential diagnoses of rickets are also considered. Controversial issues surrounding vitamin D deficiency include determination of what constitutes vitamin D sufficiency and the potential relationship between low levels of vitamin D metabolites in many individuals and unexplained fractures in infants.

  19. Canine hypoadrenocorticism: Part I

    PubMed Central

    Klein, Susan C.; Peterson, Mark E.

    2010-01-01

    Hypoadrenocorticism (Addison’s disease) has been referred to as “the great pretender,” due to its ability to mimic other common diseases in the dog and thereby represent a diagnostic challenge. Naturally occurring hypoadrenocorticism is an uncommon canine disease. Young, female dogs are overrepresented. Hypoadrenocorticism typically results from immune-mediated destruction of all adrenocortical layers, resulting in deficiencies of min-eralocorticoids (aldosterone) and glucocorticoids (cortisol). A small number of dogs suffer from glucocorticoid deficiency only. Dogs suffering from hypoadrenocorticism may present in a variety of conditions, from a mildly ill dog to a shocky and recumbent dog. This review discusses etiology, pathophysiology, history, physical examination findings, and diagnostic findings in the Addisonian patient. A follow-up article (Part II) will discuss the definitive diagnosis and management strategies for these patients. PMID:20357943

  20. 14 CFR Appendix K to Part 121 - Performance Requirements for Certain Turbopropeller Powered Airplanes

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., and meets the additional airworthiness standards in SFAR No. 23 of 14 CFR part 23. iii. Normal... chapter. iv. Normal category, and complies with section 1.(a) or 1.(b) of SFAR No. 41 of 14 CFR part 21. b... in accordance with SFAR No. 41 of 14 CFR part 21, as described in paragraph 1.a.iv of this...

  1. 14 CFR Appendix K to Part 121 - Performance Requirements for Certain Turbopropeller Powered Airplanes

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., and meets the additional airworthiness standards in SFAR No. 23 of 14 CFR part 23. iii. Normal... chapter. iv. Normal category, and complies with section 1.(a) or 1.(b) of SFAR No. 41 of 14 CFR part 21. b... in accordance with SFAR No. 41 of 14 CFR part 21, as described in paragraph 1.a.iv of this...

  2. 14 CFR Appendix K to Part 121 - Performance Requirements for Certain Turbopropeller Powered Airplanes

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., and meets the additional airworthiness standards in SFAR No. 23 of 14 CFR part 23. iii. Normal... chapter. iv. Normal category, and complies with section 1.(a) or 1.(b) of SFAR No. 41 of 14 CFR part 21. b... in accordance with SFAR No. 41 of 14 CFR part 21, as described in paragraph 1.a.iv of this...

  3. 14 CFR Appendix K to Part 121 - Performance Requirements for Certain Turbopropeller Powered Airplanes

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., and meets the additional airworthiness standards in SFAR No. 23 of 14 CFR part 23. iii. Normal... chapter. iv. Normal category, and complies with section 1.(a) or 1.(b) of SFAR No. 41 of 14 CFR part 21. b... in accordance with SFAR No. 41 of 14 CFR part 21, as described in paragraph 1.a.iv of this...

  4. 14 CFR Appendix K to Part 121 - Performance Requirements for Certain Turbopropeller Powered Airplanes

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., and meets the additional airworthiness standards in SFAR No. 23 of 14 CFR part 23. iii. Normal... chapter. iv. Normal category, and complies with section 1.(a) or 1.(b) of SFAR No. 41 of 14 CFR part 21. b... in accordance with SFAR No. 41 of 14 CFR part 21, as described in paragraph 1.a.iv of this...

  5. "Ask Argonne" - Robert Jacob, Climate Scientist, Part 2

    ScienceCinema

    Jacob, Robert

    2016-07-12

    Previously, climate scientist Robert Jacob talked a bit about the work he does and invited questions from the public during Part 1 of his "Ask Argonne" video set (http://bit.ly/1aK6WDv). In Part 2, he answers some of the questions that were submitted.

  6. "Ask Argonne" - Robert Jacob, Climate Scientist, Part 2

    SciTech Connect

    Jacob, Robert

    2014-01-08

    Previously, climate scientist Robert Jacob talked a bit about the work he does and invited questions from the public during Part 1 of his "Ask Argonne" video set (http://bit.ly/1aK6WDv). In Part 2, he answers some of the questions that were submitted.

  7. 16 CFR 1508.2 - Scope of part.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... REQUIREMENTS FOR FULL-SIZE BABY CRIBS § 1508.2 Scope of part. This part sets forth the requirements whereby full-size baby cribs (as defined in § 1508.1(a)) are not banned articles under § 1500.18(a)(13) of...

  8. 16 CFR 1508.2 - Scope of part.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REQUIREMENTS FOR FULL-SIZE BABY CRIBS § 1508.2 Scope of part. This part sets forth the requirements whereby full-size baby cribs (as defined in § 1508.1(a)) are not banned articles under § 1500.18(a)(13) of...

  9. Managing Adjunct & Part-Time Faculty for the New Millennium.

    ERIC Educational Resources Information Center

    Greive, Donald E., Ed.; Worden, Catherine A., Ed.

    The contributions in this book address issues related to the management of part-time and adjunct college faculty members, including the impact of new technology, and provide information necessary for effective management in the future. The chapters are: (1) "A Vision of the Future--From the CEO" (Roy A. Church); (2) "Managing Adjunct and Part-Time…

  10. Dual-specificity tyrosine phosphorylation-regulated kinase 1A (Dyrk1A) enhances tau expression.

    PubMed

    Qian, Wei; Jin, Nana; Shi, Jianhua; Yin, Xiaomin; Jin, Xiaoxia; Wang, Shibao; Cao, Maohong; Iqbal, Khalid; Gong, Cheng-Xin; Liu, Fei

    2013-01-01

    Microtubule-associated protein tau is found to be accumulated and aggregated in the brains of individuals with Alzheimer's disease and related tauopathies. Dual-specificity tyrosine-phosphorylation regulated kinase 1A (Dyrk1A) is overexpressed in Down syndrome and may play a critical role in the early onset of tau pathology in this disease. To investigate the effect of Dyrk1A on tau expression, we co-expressed different isoforms of tau with Dyrk1A in HEK-293FT cells and measured the mRNA and protein levels of tau using RT-PCR and Western blots, respectively. We further investigated the mechanism of regulation of tau expression by Dyrk1A. We found that Dyrk1A enhanced tau expression in a dose-dependent manner. The enhancement did not require the kinase activity of Dyrk1A. Dyrk1A increased the expression of tau isoforms containing exon 10 to a larger extent than isoforms lacking exon 10. The expression of endogenous tau in neuronal cells was also regulated by Dyrk1A, and increased tau levels were found in the brains of Ts65Dn mice that overexpress Dyrk1A due to partial trisomy of chromosome 16. Dyrk1A did not enhance tau gene transcription, but increased tau mRNA stability. These results suggest that Dyrk1A enhances tau expression by stabilizing its mRNA and provides a novel insight into the regulation of tau expression and a molecular mechanism of tauopathies. PMID:23948904

  11. Using UNIX, Part 2.

    PubMed

    Mann, J

    2001-01-01

    We talked about using 3 UNIX commands. In UNIX, there are many other options for using them. But for the most part, if you can use them like I have shown, you will be able to do everything you need. If you can learn these few points well, I think you will be better off than if I give you 50 options and leave you totally confused about when to do what. On some UNIX systems, an electronic version of the UNIX manual is on the system. This gives a lot more information about each command. However, it is a bit difficult to understand. If you want more information about any command, you can type man COMMAND, e.g., man ls. This will give you more ways to use the ls command. And remember, the command pwd tells what directory you are in, cd/directory changes to another directory, ls lists the contents of the directory you are in, ls more displays the directory contents 1 page at a time (the space bar gives you the next page), ls-al gives a detailed listing of the contents of the directory you are in, ls-al more displays them 1 page at a time (the space bar gives you the next page).

  12. Cardiovascular Health, Part 2

    PubMed Central

    Baman, Timir S.; Gupta, Sanjaya; Day, Sharlene M.

    2010-01-01

    Context: An athlete’s health may be endangered if he or she continues to compete after diagnosis of certain cardiovascular conditions. The most worrisome risk is sudden cardiac death; the annual rate in US athletes is 1 in 50 000 to 200 000. Evidence Acquisition: Part 2 of this review highlights the current guidelines and controversies surrounding compatibility of participation with a variety of cardiac conditions in competitive and recreational athletics. Data sources were limited to peer-reviewed publications from 1984 to the April 2009. Results: The guidelines published by the American College of Cardiology and the European Society of Cardiology provide a framework for safe competitive and recreational sports participation in athletes with a broad spectrum of inherited and acquired cardiovascular disorders. These guidelines are necessarily conservative because it is not currently possible to individualize risk prediction. Few data are available in many areas, particularly in the noncompetitive arena or in older athletes. Conclusions: Published national guidelines are currently the foundation governing return-to-play decisions in athletes with cardiovascular conditions. Further studies are needed to refine risk stratification algorithms to allow athletes with cardiovascular conditions to reap the health benefits of regular exercise and sports participation without undue risk. PMID:23015920

  13. Acid-sensing ion channels 1a (ASIC1a) inhibit neuromuscular transmission in female mice.

    PubMed

    Urbano, Francisco J; Lino, Noelia G; González-Inchauspe, Carlota M F; González, Laura E; Colettis, Natalia; Vattino, Lucas G; Wunsch, Amanda M; Wemmie, John A; Uchitel, Osvaldo D

    2014-02-15

    Acid-sensing ion channels (ASIC) open in response to extracellular acidosis. ASIC1a, a particular subtype of these channels, has been described to have a postsynaptic distribution in the brain, being involved not only in ischemia and epilepsy, but also in fear and psychiatric pathologies. High-frequency stimulation of skeletal motor nerve terminals (MNTs) can induce presynaptic pH changes in combination with an acidification of the synaptic cleft, known to contribute to muscle fatigue. Here, we studied the role of ASIC1a channels on neuromuscular transmission. We combined a behavioral wire hanging test with electrophysiology, pharmacological, and immunofluorescence techniques to compare wild-type and ASIC1a lacking mice (ASIC1a (-/-) knockout). Our results showed that 1) ASIC1a (-/-) female mice were weaker than wild type, presenting shorter times during the wire hanging test; 2) spontaneous neurotransmitter release was reduced by ASIC1a activation, suggesting a presynaptic location of these channels at individual MNTs; 3) ASIC1a-mediated effects were emulated by extracellular local application of acid saline solutions (pH = 6.0; HEPES/MES-based solution); and 4) immunofluorescence techniques revealed the presence of ASIC1a antigens on MNTs. These results suggest that ASIC1a channels might be involved in controlling neuromuscular transmission, muscle contraction and fatigue in female mice.

  14. Acid-sensing ion channels 1a (ASIC1a) inhibit neuromuscular transmission in female mice

    PubMed Central

    Lino, Noelia G.; González-Inchauspe, Carlota M. F.; González, Laura E.; Colettis, Natalia; Vattino, Lucas G.; Wunsch, Amanda M.; Wemmie, John A.; Uchitel, Osvaldo D.

    2013-01-01

    Acid-sensing ion channels (ASIC) open in response to extracellular acidosis. ASIC1a, a particular subtype of these channels, has been described to have a postsynaptic distribution in the brain, being involved not only in ischemia and epilepsy, but also in fear and psychiatric pathologies. High-frequency stimulation of skeletal motor nerve terminals (MNTs) can induce presynaptic pH changes in combination with an acidification of the synaptic cleft, known to contribute to muscle fatigue. Here, we studied the role of ASIC1a channels on neuromuscular transmission. We combined a behavioral wire hanging test with electrophysiology, pharmacological, and immunofluorescence techniques to compare wild-type and ASIC1a lacking mice (ASIC1a −/− knockout). Our results showed that 1) ASIC1a −/− female mice were weaker than wild type, presenting shorter times during the wire hanging test; 2) spontaneous neurotransmitter release was reduced by ASIC1a activation, suggesting a presynaptic location of these channels at individual MNTs; 3) ASIC1a-mediated effects were emulated by extracellular local application of acid saline solutions (pH = 6.0; HEPES/MES-based solution); and 4) immunofluorescence techniques revealed the presence of ASIC1a antigens on MNTs. These results suggest that ASIC1a channels might be involved in controlling neuromuscular transmission, muscle contraction and fatigue in female mice. PMID:24336653

  15. Parts and boundaries.

    PubMed

    Jackendoff, R

    1991-12-01

    Within the framework of Conceptual Semantics, a family of conceptual features and functions is developed that accounts for phenomena in the semantics of noun phrases such as the mass-count distinction, plurality, the partitive construction (a leg of the table), the constitutive construction (a house of wood), the "Universal Packager" (three coffees), and boundary words such as end, edge, and crust. Using the strong formal parallelism between noun phrase semantics and event structure that is a hallmark of the Conceptual Semantics approach, the features and functions of the NP system are applied to a wide range of problems in event structure, for example the analysis of the Vendler classes, the meaning of the progressive, the "imperfective paradox", and "aktionsarten" such as the syntactically unexpressed sense of repetition in The light flashed until dawn. Crucial to the analysis is that these features and functions can be expressed in syntactic structure either by being part of lexical conceptual structure, or by use of a morphological affix, or by being associated with the meaning of a construction such as N of NP or nominal compounding. Alternatively, they may remain unexpressed altogether, being introduced into the conceptual structure of a phrase by "rules of construal". This shows that lexical semantics and phrasal semantics interpenetrate deeply, and that there is no strict one-to-one correspondence between syntactic and semantic structures. In addition, the analysis provides further evidence that natural language semantics must be based on a psychological view of meaning--it must be concerned with how language users are constructed to understand and schematize the world. PMID:1790657

  16. 9. SITE OVERVIEW. PART 4 OF 6 PART PANORAMA WITH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. SITE OVERVIEW. PART 4 OF 6 PART PANORAMA WITH NOS. CA-265-6 TO CA-265-11. ARROYO SECO PARKWAY AS SEEN FROM RADIO TOWER HILL. PART 4 SHOWS ARROYO SECO PARKWAY IN FOREGROUND AND FIGUEROA STREET AT CENTER. LOOKING 316° NW. - Arroyo Seco Parkway, Los Angeles to Pasadena, Los Angeles, Los Angeles County, CA

  17. X-1A in flight over lakebed

    NASA Technical Reports Server (NTRS)

    1953-01-01

    The Bell Aircraft Corporation X-1A (48-1384) returning from an Air Force test flight over Edwards Air Force Base, California in late 1953. A North American F-86A Sabre as chase plane will follow the X-1A to touchdown. The Rogers Dry Lake is the whitish area under the planes with the airfield at the edge of the dry lake. Bell test pilot Jean 'Skip' Ziegler made six flights between 14 February and 25 April 1953. Air Force test pilots Maj. Charles 'Chuck' Yeager and Maj. Arthur 'Kit' Murray made 18 test flights between 21 November 1953 and 26 August 1954. NACA test pilot Joseph Walker made one successful flight on 20 July 1955. During a second flight attempt, on 8 August 1955, an explosion damaged the aircraft shortly before launch. Walker, unhurt, climbed up into the JTB-29A mothership, and the X-1A was jettisoned over the Edwards AFB bombing range. There were five versions of the Bell X-1 rocket-powered research aircraft that flew at the NACA High-Speed Flight Research Station, Edwards, California. The bullet-shaped X-1 aircraft were built by Bell Aircraft Corporation, Buffalo, N.Y. for the U.S. Army Air Forces (after 1947, U.S. Air Force) and the National Advisory Committee for Aeronautics (NACA). The X-1 Program was originally designated the XS-1 for EXperimental Sonic. The X-1's mission was to investigate the transonic speed range (speeds from just below to just above the speed of sound) and, if possible, to break the 'sound barrier.' Three different X-1s were built and designated: X-1-1, X-1-2 (later modified to become the X-1E), and X-1-3. The basic X-1 aircraft were flown by a large number of different pilots from 1946 to 1951. The X-1 Program not only proved that humans could go beyond the speed of sound, it reinforced the understanding that technological barriers could be overcome. The X-1s pioneered many structural and aerodynamic advances including extremely thin, yet extremely strong wing sections; supersonic fuselage configurations; control system

  18. Cryogenic Treatment of Metal Parts

    SciTech Connect

    Chillar, Rahul; Agrawal, S. C.

    2006-03-31

    Cryogenic treatment and its variables have been described. Results of eight engineering tests carried out on cryotreated parts have been presented. Cryogenic treatment of metal parts enhances useful properties which in turn, improves various strengths. Our tests viz. Abrasion, Torsion, Fatigue, Tensile, Shear, Hardness and Impact on Mild steel, Cast Iron, Brass and Copper show that the cryogenic treatment improved useful properties of mild steel parts appreciably but did not show promise with brass and copper parts.

  19. [Physicians working part-time].

    PubMed

    Kiechl-Kohlendorfer, Ursula; Hackl, Johann Michael

    2004-04-01

    In order to ensure the best possible medical care and high-quality research, hospital medical staff must be well educated and highly motivated. Part-time work can help to provide good human resources. Moreover, part-time jobs are often necessary as a means of juggling work and family responsibilities. The aim of this study was to illustrate the legal and educational groundwork for part-time work for physicians in Austria and to outline the advantages and drawbacks of part-time work. In March 2003, all medical doctors working part-time at hospitals in the Austrian state of the Tyrol (n = 60) were surveyed by means of a written questionnaire with the aim of finding out their motives for taking a part-time job as well as the positive and negative aspects involved. The response rate was nearly 70% (n = 40). Most of the part-time hospital physicians were female. The vast majority chose part-time employment in order to better balance family and job. Problems involved in part-time work are legal issues (part-time employment does not always qualify for medical training), the comparatively low salary and the fewer career opportunities. All respondents agreed that patient care, teaching and research are not compatible on a part-time basis. Another negative aspect is the generally strong time pressure experienced in part-time jobs. Despite these negative aspects of working part-time, advantages are considered more important, especially the better balancing of job and family and the possibility of an important early professional reintegration. Better acceptance of part-time jobs would help to make part-time work more attractive. An important improvement is associated with the forthcoming changes in the laws governing part-time work and medical training. PMID:15182044

  20. Air Pollution. Part A: Analysis.

    ERIC Educational Resources Information Center

    Ledbetter, Joe O.

    Two facets of the engineering control of air pollution (the analysis of possible problems and the application of effective controls) are covered in this two-volume text. Part A covers Analysis, and Part B, Prevention and Control. (This review is concerned with Part A only.) This volume deals with the terminology, methodology, and symptomatology…

  1. Exploring Water Pollution. Part II

    ERIC Educational Resources Information Center

    Rillo, Thomas J.

    1975-01-01

    This is part two of a three part article related to the science activity of exploring environmental problems. Part one dealt with background information for the classroom teacher. Presented here is a suggested lesson plan on water pollution. Objectives, important concepts and instructional procedures are suggested. (EB)

  2. [MUC1, a therapeutic target in oncology].

    PubMed

    Limacher, Jean-Marc; Acres, Bruce

    2007-03-01

    MUC1 is a large, highly glycosylated protein expressed on the apical membrane of many epithelial cells. With other members of the mucin family it contributes to the protection and function of mucosal cells. The intracellular part of the protein may also participate in signal transduction pathway, through multiple interactions with intracellular proteins. Overexpression of MUC1 is frequently observed in the majority of epithelial cancers and even in some haematological malignancies. In tumor cells, MUC1 loses apical distribution and is hypoglycosylated. These cancer-associated changes render it antigenic and make it an attractive target for a specific cancer immunotherapy. Several MUC1-based therapeutic cancer vaccines are currently under clinical investigation. PMID:17371767

  3. (1) (1)A' ← X (1)A' Electronic Transition of Protonated Coronene at 15 K.

    PubMed

    Rice, C A; Hardy, F-X; Gause, O; Maier, J P

    2014-03-20

    The electronic spectrum of protonated coronene in the gas phase was measured at vibrational and rotational temperatures of ∼15 K in a 22-pole ion trap. The (1) (1)A' ← X (1)A' electronic transition of this larger polycyclic aromatic hydrocarbon cation has an origin band maximum at 14 383.8 ± 0.2 cm(-1) and shows distinct vibrational structure in the (1) (1)A' state. Neither the origin nor the strongest absorptions to the blue coincide with known diffuse interstellar bands, implying that protonated coronene is not a carrier.

  4. Total syntheses of disulphated glycosphingolipid SB1a and the related monosulphated SM1a

    PubMed Central

    Hirose, Haruka; Tamai, Hideki; Gao, Chao; Imamura, Akihiro; Ando, Hiromune; Ishida, Hideharu; Feizi, Ten; Kiso, Makoto

    2016-01-01

    Total syntheses of two natural sulphoglycolipids, disulphated glycosphingolipid SB1a and the structurally related monosulphated SM1a, are described. They have common glycan sequences and ceramide moiety and are associated with human epithelial carcinomas. The syntheses featured efficient glycan assembly and the glucosyl ceramide cassette as a versatile building block. The binding of the synthetic sulphoglycolipids by the carcinoma-specific monoclonal antibody AE3 was investigated using carbohydrate microarray technology. PMID:26399908

  5. Recognition of platinum-DNA adducts by HMGB1a.

    PubMed

    Ramachandran, Srinivas; Temple, Brenda; Alexandrova, Anastassia N; Chaney, Stephen G; Dokholyan, Nikolay V

    2012-09-25

    Cisplatin (CP) and oxaliplatin (OX), platinum-based drugs used widely in chemotherapy, form adducts on intrastrand guanines (5'GG) in genomic DNA. DNA damage recognition proteins, transcription factors, mismatch repair proteins, and DNA polymerases discriminate between CP- and OX-GG DNA adducts, which could partly account for differences in the efficacy, toxicity, and mutagenicity of CP and OX. In addition, differential recognition of CP- and OX-GG adducts is highly dependent on the sequence context of the Pt-GG adduct. In particular, DNA binding protein domain HMGB1a binds to CP-GG DNA adducts with up to 53-fold greater affinity than to OX-GG adducts in the TGGA sequence context but shows much smaller differences in binding in the AGGC or TGGT sequence contexts. Here, simulations of the HMGB1a-Pt-DNA complex in the three sequence contexts revealed a higher number of interface contacts for the CP-DNA complex in the TGGA sequence context than in the OX-DNA complex. However, the number of interface contacts was similar in the TGGT and AGGC sequence contexts. The higher number of interface contacts in the CP-TGGA sequence context corresponded to a larger roll of the Pt-GG base pair step. Furthermore, geometric analysis of stacking of phenylalanine 37 in HMGB1a (Phe37) with the platinated guanines revealed more favorable stacking modes correlated with a larger roll of the Pt-GG base pair step in the TGGA sequence context. These data are consistent with our previous molecular dynamics simulations showing that the CP-TGGA complex was able to sample larger roll angles than the OX-TGGA complex or either CP- or OX-DNA complexes in the AGGC or TGGT sequences. We infer that the high binding affinity of HMGB1a for CP-TGGA is due to the greater flexibility of CP-TGGA compared to OX-TGGA and other Pt-DNA adducts. This increased flexibility is reflected in the ability of CP-TGGA to sample larger roll angles, which allows for a higher number of interface contacts between the Pt

  6. D-1A equipment module structure test

    NASA Technical Reports Server (NTRS)

    Niezgoda, T. F.

    1976-01-01

    The Centaur Equipment Module (E/M) structural test program was performed in two parts due to an unscheduled hardware failure in the first test series. The objectives of the initial test program were to define the flexibility characteristics of the E/M, verify the design load capability, and determine its ultimate strength capability by loading to structural failure. However, during the first failure test attempt, the Intelsat IV MPA failed instead of the E/M. Therefore a new adapter was fabricated to simulate the HEAO mission adapter and the second series of tests were then performed. They concluded with the failure of the E/M forward interface ring resulting in about 3.5 degrees of permanent set on the high compression side. Nevertheless, the linear or useable strength capability of the E/M is greater or equal to that which is required for the HEAO missions. The E/M is deemed structurally qualified for the HEAO missions.

  7. Heme Oxygenase-1: A Metabolic Nike

    PubMed Central

    Nemeth, Zsuzsanna; Correa-Costa, Matheus; Bulmer, Andrew C.; Otterbein, Leo E.

    2014-01-01

    Abstract Significance: Heme degradation, which was described more than 30 years ago, is still very actively explored with many novel discoveries on its role in various disease models every year. Recent Advances: The heme oxygenases (HO) are metabolic enzymes that utilize NADPH and oxygen to break apart the heme moiety liberating biliverdin (BV), carbon monoxide (CO), and iron. Heme that is derived from hemoproteins can be toxic to the cells and if not removed immediately, it causes cell apoptosis and local inflammation. Elimination of heme from the milieu enables generation of three products that influences numerous metabolic changes in the cell. Critical Issues: CO has profound effects on mitochondria and cellular respiration and other hemoproteins to which it can bind and affect their function, while BV and bilirubin (BR), the substrate and product of BV, reductase, respectively, are potent antioxidants. Sequestration of iron into ferritin and its recycling in the tissues is a part of the homeodynamic processes that control oxidation-reduction in cellular metabolism. Further, heme is an important component of a number of metabolic enzymes, and, therefore, HO-1 plays an important role in the modulation of cellular bioenergetics. Future Directions: In this review, we describe the cross-talk between heme oxygenase-1 (HO-1) and its products with other metabolic pathways. HO-1, which we have labeled Nike, the goddess who personified victory, dictates triumph over pathophysiologic conditions, including diabetes, ischemia, and cancer. Antioxid. Redox Signal. 20, 1709–1722. PMID:24180257

  8. A study of LC-39 cryogenic systems. Part 1: A study of the vacuum insulated transfer lines at Kennedy Space Center. Part 2: Cooldown pressure surges

    NASA Technical Reports Server (NTRS)

    Ludtke, P. R.; Voth, R. O.

    1971-01-01

    The vacuum liquid hydrogen and liquid oxygen transfer lines at Kennedy Space Center were studied to evaluate the feasibility of using a condensing gas such as CO2 inside the vacuum spaces to achieve a condensing-vacuum. The study indicates that at ambient temperature, a maximum vacuum hyphen space pressure of 4000 microns is acceptable for the LH2 transfer lines. In addition, the cooldown procedures for the 14-inch cross-country liquid oxygen line was studied using a simplified mathematical model. Preliminary cooldown times are presented for various heat leak rates to the line and for two vent configurations.

  9. Lack of CYP1A responsiveness in species inhabiting chronically contaminated habitats: two varieties of resistance?

    PubMed

    Brammell, Ben F; Price, David J; Birge, Wesley J; Elskus, Adria A

    2013-03-01

    PCBs in a species within the family Ictaluridae and provides further support for our previous work indicating an apparent inherent lack of CYP1A sensitivity to chlorinated inducers in Centrarchids. These traits may explain, at least in part, the common association of these families with degraded habitats and indicate Lepomis members are likely to be excellent candidates for exploring the mechanistic basis of 'inherent' CYP1A resistance. This study also underlines to the need for thorough characterization of the CYP1A responsivity of a population and/or species prior to using CYP1A as a reliable biomonitoring tool.

  10. Association of the arginine vasopressin receptor 1A (AVPR1A) haplotypes with listening to music.

    PubMed

    Ukkola-Vuoti, Liisa; Oikkonen, Jaana; Onkamo, Päivi; Karma, Kai; Raijas, Pirre; Järvelä, Irma

    2011-04-01

    Music is listened in all cultures. We hypothesize that willingness to produce and perceive sound and music is social communication that needs musical aptitude. Here, listening to music was surveyed using a web-based questionnaire and musical aptitude using the auditory structuring ability test (Karma Music test) and Carl Seashores tests for pitch and for time. Three highly polymorphic microsatellite markers (RS3, RS1 and AVR) of the arginine vasopressin receptor 1A (AVPR1A) gene, previously associated with social communication and attachment, were genotyped and analyzed in 31 Finnish families (n=437 members) using family-based association analysis. A positive association between the AVPR1A haplotype (RS1 and AVR) and active current listening to music (permuted P=0.0019) was observed. Other AVPR1A haplotype (RS3 and AVR) showed association with lifelong active listening to music (permuted P=0.0022). In addition to AVPR1A, two polymorphisms (5-HTTLPR and variable number of tandem repeat) of human serotonin transporter gene (SLC6A4), a candidate gene for many neuropsychiatric disorders and previously associated with emotional processing, were analyzed. No association between listening to music and the polymorphisms of SLC6A4 were detected. The results suggest that willingness to listen to music is related to neurobiological pathways affecting social affiliation and communication.

  11. Boiler - tuning basics, part 1

    SciTech Connect

    Leopold, T.

    2009-03-15

    Tuning power plant controls takes nerves of steel and an intimate knowledge of plant systems gained only by experience. Tuning controls also requires equal parts art and science, which probably is why there are so few tuning experts in the power industry. In part 1 of a two-part series, the author explores a mix of the theoretical and practical aspects of tuning boiler control. 5 figs.

  12. Water Pollution: Part I, Municipal Wastewaters; Part II, Industrial Wastewaters.

    ERIC Educational Resources Information Center

    Fowler, K. E. M.

    This publication is an annotated bibliography of municipal and industrial wastewater literature. This publication consists of two parts plus appendices. Part one is entitled Municipal Wastewaters and includes publications in such areas as health effects of polluted waters, federal policy and legislation, biology and chemistry of polluted water,…

  13. How relevant is the deposition of mercury onto snowpacks? - Part 1: A statistical study on the impact of environmental factors

    NASA Astrophysics Data System (ADS)

    Durnford, D. A.; Dastoor, A. P.; Steen, A. O.; Berg, T.; Ryzhkov, A.; Figueras-Nieto, D.; Hole, L. R.; Pfaffhuber, K. A.; Hung, H.

    2012-01-01

    A portion of the highly toxic methylmercury that bioaccumulates in aquatic life is created from mercury entering bodies of water with snowpack meltwater. To determine the importance of meltwater as a source of aquatic mercury, it is necessary to understand the environmental processes that govern the behavior of snowpack-related mercury. In this study we investigate relationships among 5 types of snowpack-related mercury observations and 20 model environmental variables. The observation types are the 24-h fractional loss of mercury from surface snow, and the concentrations of mercury in surface snow, seasonal snowpacks, the snowpack meltwater's ionic pulse, and long-term snowpack-related records. The model environmental variables include those related to atmospheric mercury, insolation, wind, atmospheric stability, snowpack physical characteristics, atmospheric pressure, and solid precipitation. Correlation coefficients and multiple linear regressions were calculated twice: once with all observations, and once with observations from locations presumably affected by oxidizing and stabilizing snowpack-related halogens excluded. We find that the presence of snowpack-related halogens has a significant impact on the behavior of snowpack-related mercury. Physically, snowpack-related mercury observations are most strongly controlled by the dry and wet depositions of oxidized mercury. The burial of mercury by fresh snowfalls and the wind driven ventilation of snowpacks are important processes. Indeed, in the absence of snowpack-related halogens, the 24-h fractional loss of mercury from surface snow is fully controlled by mercury deposition and surface-level atmospheric wind speed, stability, and surface pressure. The concentration of mercury in long-term records is affected by latitude, ventilation and surface pressure.

  14. Patient-practitioner-remedy (PPR) entanglement. Part 1: a qualitative, non-local metaphor for homeopathy based on quantum theory.

    PubMed

    Milgrom, L R

    2002-10-01

    A metaphor for homeopathy is developed in which the potentised medicine, the patient, and the practitioner are seen as forming a non-local therapeutically 'entangled' triad, qualitatively described in terms of the transactional interpretation of quantum mechanics.

  15. Aggregation of measures to produce an overall assessment of animal welfare. Part 1: a review of existing methods.

    PubMed

    Botreau, R; Bonde, M; Butterworth, A; Perny, P; Bracke, M B M; Capdeville, J; Veissier, I

    2007-09-01

    Several systems have been proposed for the overall assessment of animal welfare at the farm level for the purpose of advising farmers or assisting public decision-making. They are generally based on several measures compounded into a single evaluation, using different rules to assemble the information. Here we discuss the different methods used to aggregate welfare measures and their applicability to certification schemes involving welfare. Data obtained on a farm can be (i) analysed by an expert who draws an overall conclusion; (ii) compared with minimal requirements set for each measure; (iii) converted into ranks, which are then summed; or (iv) converted into values or scores compounded in a weighted sum (e.g. TGI35L) or using ad hoc rules. Existing methods used at present (at least when used exclusively) may be insufficiently sensitive or not routinely applicable, or may not reflect the multidimensional nature of welfare and the relative importance of various welfare measures. It is concluded that different methods may be used at different stages of the construction of an overall assessment of animal welfare, depending on the constraints imposed on the aggregation process.

  16. Numerical simulations of piano strings. Part 1: A physical model for a struck string using finite difference methods

    NASA Astrophysics Data System (ADS)

    Chaigne, Antoine; Askenfelt, Anders

    1993-04-01

    An improvement to the numerical approach and the underlying physical model made by Hiller and Ruiz in their attempt to generate musical sounds by solving the equations of vibrating strings by means of Finite Difference Methods (FDM) in order to simulate the notion of the piano string with a high degree of realism, is shown. Starting from the fundamental equations of a damped, stiff string interacting with a nonlinear hammer, a numerical finite difference scheme is derived, from which the time histories of string displacement and velocity for each point of the string are computed in the time domain. The interacting force between hammer and string, and the force acting on the bridge, are given by the same scheme. The performance of the model is illustrated by examples of simulated string waveforms. Aspects of numerical stability and dispersion are discussed with reference to the proper choice of sampling parameters.

  17. The clinician in the Driver's Seat: part 1 - a drag/drop user-composable electronic health record platform.

    PubMed

    Senathirajah, Yalini; Bakken, Suzanne; Kaufman, David

    2014-12-01

    Creating electronic health records that support the uniquely complex and varied needs of healthcare presents formidable challenges. To address some of these challenges we created a new model for healthcare information systems, embodied in MedWISE,(2) a widget-based highly configurable electronic health record (EHR) platform. Founded on the idea that providing clinician users with greater control of the EHR may result in greater fit to user needs and preferences, MedWISE allows drag/drop user configurations and the sharing of user-created elements such as custom laboratory result panels and user-created interface tabs. After reviewing the current state of EHR configurability, we describe the philosophical, theoretical and practical rationales for our model, and the specific functionality of MedWISE. The alternative approach may have several advantages for human-computer interaction, efficiency, cognition, and fit of EHR tools to different contexts and tasks. We discuss potential issues raised by this approach.

  18. Current distribution in a three-dimensional IC analyzed by a perturbation method. Part 1: A simple steady state theory

    NASA Technical Reports Server (NTRS)

    Edmonds, Larry D.

    1987-01-01

    The steady state current distribution in a three dimensional integrated circuit is presented. A device physics approach, based on a perturbation method rather than an equivalent lumped circuit approach, is used. The perturbation method allows the various currents to be expressed in terms of elementary solutions which are solutions to very simple boundary value problems. A Simple Steady State Theory is the subtitle because the most obvious limitation of the present version of the analysis is that all depletion region boundary surfaces are treated as equipotential surfaces. This may be an adequate approximation in some applications but it is an obvious weakness in the theory when applied to latched states. Examples that illustrate the use of these analytical methods are not given because they will be presented in detail in the future.

  19. Diagnosing the average spatio-temporal impact of convective systems - Part 1: A methodology for evaluating climate models

    NASA Astrophysics Data System (ADS)

    Johnston, M. S.; Eriksson, P.; Eliasson, S.; Zelinka, M. D.; Forbes, R. M.; Wyser, K.

    2013-05-01

    A~method to determine the mean response of upper tropospheric water to localised deep convective (DC) events is improved and applied to the EC-Earth climate model. Following Zelinka and Hartmann (2009), several fields related to moist processes and radiation are composited with respect to local maxima in rain rate to determine their spatio-temporal evolution with deep convection in the central Pacific Ocean. Major improvements to the above study are the isolation of DC events in time so as to prevent multiple sampling of the same event, and a revised definition of the mean background state that allows for better characterization of the DC-induced anomalies. The DC events observed in this study propagate westward at ~ 4 m s-1. Both the upper tropospheric relative humidity and outgoing longwave radiation are substantially perturbed over a broad horizontal extent during peak convection and for long periods of time. Cloud fraction anomaly increases throughout the upper troposphere, especially in the 200-250 hPa layer, reaching peak coverage following deep convection. Cloud ice water content anomaly confined to pressures greater than about 250 hPa and peaks near 450 hPa within a few hours of the DC event but remain enhanced following the DC event. Consistent with the large increase in upper tropospheric cloud ice, albedo increases dramatically and persists for sometime following the DC event. Applying the method to the model demonstrates that it is able to capture the large-scale responses to DC events, most notably for outgoing longwave radiation, but there are a number of important differences. For example, the DC signature of upper tropospheric humidity consistently covers a broader horizontal area than what is observed. In addition, the DC events move eastward in the model, but westward in the observations, and exhibit an unrealistic 24 h repeat cycle. Moreover, the modeled upper tropospheric cloud fraction anomalies - despite being of comparable magnitude and exhibiting similar longevity - are confined to a thinner layer that is closer to the tropopause and peak earlier than in observations. Finally, the modeled ice water content anomalies at pressures greater than about 350 hPa are about twice as large as in the observations and do not persist as long after peak convection.

  20. Diagnosing the average spatio-temporal impact of convective systems - Part 1: A methodology for evaluating climate models

    NASA Astrophysics Data System (ADS)

    Johnston, M. S.; Eliasson, S.; Eriksson, P.; Forbes, R. M.; Wyser, K.; Zelinka, M. D.

    2013-12-01

    An earlier method to determine the mean response of upper-tropospheric water to localised deep convective systems (DC systems) is improved and applied to the EC-Earth climate model. Following Zelinka and Hartmann (2009), several fields related to moist processes and radiation from various satellites are composited with respect to the local maxima in rain rate to determine their spatio-temporal evolution with deep convection in the central Pacific Ocean. Major improvements to the earlier study are the isolation of DC systems in time so as to prevent multiple sampling of the same event, and a revised definition of the mean background state that allows for better characterisation of the DC-system-induced anomalies. The observed DC systems in this study propagate westward at ~4 m s-1. Both the upper-tropospheric relative humidity and the outgoing longwave radiation are substantially perturbed over a broad horizontal extent and for periods >30 h. The cloud fraction anomaly is fairly constant with height but small maximum can be seen around 200 hPa. The cloud ice water content anomaly is mostly confined to pressures greater than 150 hPa and reaches its maximum around 450 hPa, a few hours after the peak convection. Consistent with the large increase in upper-tropospheric cloud ice water content, albedo increases dramatically and persists about 30 h after peak convection. Applying the compositing technique to EC-Earth allows an assessment of the model representation of DC systems. The model captures the large-scale responses, most notably for outgoing longwave radiation, but there are a number of important differences. DC systems appear to propagate eastward in the model, suggesting a strong link to Kelvin waves instead of equatorial Rossby waves. The diurnal cycle in the model is more pronounced and appears to trigger new convection further to the west each time. Finally, the modelled ice water content anomaly peaks at pressures greater than 500 hPa and in the upper troposphere between 250 hPa and 500 hPa, there is less ice than the observations and it does not persist as long after peak convection. The modelled upper-tropospheric cloud fraction anomaly, however, is of a comparable magnitude and exhibits a similar longevity as the observations.

  1. Microbial carbon recycling - an underestimated process controlling soil carbon dynamics - Part 1: A long-term laboratory incubation experiment

    NASA Astrophysics Data System (ADS)

    Basler, A.; Dippold, M.; Helfrich, M.; Dyckmans, J.

    2015-10-01

    Independent of its chemical structure carbon (C) persists in soil for several decades, controlled by stabilization and recycling. To disentangle the importance of the two factors on the turnover dynamics of soil sugars, an important compound of soil organic matter (SOM), a 3-year incubation experiment was conducted on a silty loam soil under different types of land use (arable land, grassland and forest) by adding 13C-labelled glucose. The compound-specific isotope analysis of soil sugars was used to examine the dynamics of different sugars during incubation. Sugar dynamics were dominated by a pool of high mean residence times (MRT) indicating that recycling plays an important role for sugars. However, this was not substantially affected by soil C content. Six months after label addition the contribution of the label was much higher for microbial biomass than for CO2 production for all examined land use types, corroborating that substrate recycling was very effective within the microbial biomass. Two different patterns of tracer dynamics could be identified for different sugars: while fucose and mannose showed highest label contribution at the beginning of the incubation with a subsequent slow decline, galactose and rhamnose were characterized by slow label incorporation with subsequently constant levels, which indicates that recycling is dominating the dynamics of these sugars. This may correspond to (a) different microbial growing strategies (r and K-strategist) or (b) location within or outside the cell membrane (lipopolysaccharides vs. exopolysaccharides) and thus be subject of different re-use within the microbial food web. Our results show how the microbial community recycles substrate very effectively and that high losses of substrate only occur during initial stages after substrate addition. This study indicates that recycling is one of the major processes explaining the high MRT observed for many SOM fractions and thus is crucial for understanding the global soil C cycle.

  2. Hybrid clustering based fuzzy structure for vibration control - Part 1: A novel algorithm for building neuro-fuzzy system

    NASA Astrophysics Data System (ADS)

    Nguyen, Sy Dzung; Nguyen, Quoc Hung; Choi, Seung-Bok

    2015-01-01

    This paper presents a new algorithm for building an adaptive neuro-fuzzy inference system (ANFIS) from a training data set called B-ANFIS. In order to increase accuracy of the model, the following issues are executed. Firstly, a data merging rule is proposed to build and perform a data-clustering strategy. Subsequently, a combination of clustering processes in the input data space and in the joint input-output data space is presented. Crucial reason of this task is to overcome problems related to initialization and contradictory fuzzy rules, which usually happen when building ANFIS. The clustering process in the input data space is accomplished based on a proposed merging-possibilistic clustering (MPC) algorithm. The effectiveness of this process is evaluated to resume a clustering process in the joint input-output data space. The optimal parameters obtained after completion of the clustering process are used to build ANFIS. Simulations based on a numerical data, 'Daily Data of Stock A', and measured data sets of a smart damper are performed to analyze and estimate accuracy. In addition, convergence and robustness of the proposed algorithm are investigated based on both theoretical and testing approaches.

  3. Test design description for the Fusion Materials Open Test Assembly (Fusion MOTA-2A): Volume 1A, Part 1

    SciTech Connect

    Bauer, R.E.

    1988-11-01

    This document encompasses the test requirements, hardware design, fabrication, and safety analysis for the Fusion Materials Open Test Assembly experiment for irradiation in FFTF Cycle 11 (Fusion MOTA-2A). Fusion MOTA is equally shared by the US Fusion Material (DOE), Japanese Fusion Materials (MONBUSHO), and BEATRIX-II (IEA) programs. In the interest of providing optimum use of the irradiation space in the Fusion MOTA-2A and LMR MOTA-1G, eight of the Fusion MOTA canisters will be placed in MOTA-1G and an equal number of LMR canisters placed in Fusion MOTA-2A (Powell/Doran 1988). This eliminates the need to process Fusion MOTA-2A through the IEM cell prior to insertion for FFTF Cycle 11A. The LMR MOTA design and safety analysis (Greenslade 1984) is the basis for much of this design and safety analysis report. This design description and safety analysis for the Fusion MOTA-2A is presented per the outline given in Chapter IV of the FTR User`s Guide (Taylor 1978). 35 refs., 17 figs., 9 tabs.

  4. A modified milling system, using a bimodal distribution of highly resistant ceramics. Part 1. A natural hydroxyapatite study.

    PubMed

    Harabi, Abdelhamid; Harabi, Esma

    2015-06-01

    A careful combination of the main parameters controlling natural hydroxyapatite (NHA: Ca10(PO4)6(OH)2) production such as milling techniques, sintering temperature and holding time may lead to an interesting NHA based bio-ceramics without any foreign oxide additions. In this way, an original wet milling setup has been proposed to obtain sub-micron sized NHA powders. In order to avoid any possible NHA decomposition, a precise Ca/P ratio of NHA derived from animals was selected accordingly. Also, an alternative direct visual approach of the bone age classification was also proposed. A relative density of about 95% was obtained for powders sintered at 1300°C for 2h. The best Vickers micro-hardness and 3 point bending strength values for these sintered samples, using this proposed milling system and without any additions, were 4.7±0.3GPa and 37MPa, respectively. Finally, a complete correlation between the powder microstructure and the final product properties has been established.

  5. 40 CFR Table 2 to Subpart Jjjj of... - Applicability of 40 CFR Part 63 General Provisions to Subpart JJJJ

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 12 2010-07-01 2010-07-01 true Applicability of 40 CFR Part 63 General... Web Coating Part 63, Subpt. JJJJ, Table 2 Table 2 to Subpart JJJJ of Part 63—Applicability of 40 CFR... Explanation § 63.1(a)(1)-(4) Yes. § 63.1(a)(5) No Reserved. § 63.1(a)(6)-(8) Yes. § 63.1(a)(9) No...

  6. 40 CFR Table 1a to Subpart Ce of... - Emissions Limits for Small, Medium, and Large HMIWI at Designated Facilities as Defined in § 60...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Large HMIWI at Designated Facilities as Defined in § 60.32e(a)(1) 1A Table 1A to Subpart Ce of Part 60.../Infectious Waste Incinerators Pt. 60, Subpt. Ce, Table 1A Table 1A to Subpart Ce of Part 60—Emissions Limits... Units (7 percent oxygen, dry basis) Emissions limits HMIWI size Small Medium Large Averaging time...

  7. 40 CFR Table 1a to Subpart Ce of... - Emissions Limits for Small, Medium, and Large HMIWI at Designated Facilities as Defined in § 60...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Large HMIWI at Designated Facilities as Defined in § 60.32e(a)(1) 1A Table 1A to Subpart Ce of Part 60.../Infectious Waste Incinerators Pt. 60, Subpt. Ce, Table 1A Table 1A to Subpart Ce of Part 60—Emissions Limits... Units (7 percent oxygen, dry basis) Emissions limits HMIWI size Small Medium Large Averaging time...

  8. 40 CFR Table 1a to Subpart Ce of... - Emissions Limits for Small, Medium, and Large HMIWI at Designated Facilities as Defined in § 60...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Large HMIWI at Designated Facilities as Defined in § 60.32e(a)(1) 1A Table 1A to Subpart Ce of Part 60.../Infectious Waste Incinerators Pt. 60, Subpt. Ce, Table 1A Table 1A to Subpart Ce of Part 60—Emissions Limits... Units (7 percent oxygen, dry basis) Emissions limits HMIWI size Small Medium Large Averaging time...

  9. Detecting Earthquakes--Part 2.

    ERIC Educational Resources Information Center

    Isenberg, C.; And Others

    1983-01-01

    Basic concepts associated with seismic wave propagation through the earth and the location of seismic events were explained in part 1 (appeared in January 1983 issue). This part focuses on the construction of a student seismometer for detecting earthquakes and underground nuclear explosions anywhere on the earth's surface. (Author/JN)

  10. Intercultural Communication Bibliography (Part 4).

    ERIC Educational Resources Information Center

    Kitao, Kenji; Kitao, S. Kathleen

    The fourth part of a bibliography on intercultural communication is, like the first three parts, intended primarily for Japanese teachers of English as a second language but is also useful for anyone interested in intercultural communication. The bibliography contains 480 citations of books, journal articles, and other sources for information…

  11. "Leading Parts" and Organizational Change.

    ERIC Educational Resources Information Center

    Brown, L. Dave

    This paper develops a conceptual framework for identifying organizational change and applies the framework to understanding organizational change. The main focus of the framework is the concept of "leading parts." Leading parts may be dominant or catalytic in their impact on systems; and understanding their operation requires analysis of the…

  12. The Moonwatch Programme, Part 8

    NASA Astrophysics Data System (ADS)

    Roberts, Greg

    2015-08-01

    This part deals with the satellite tracking done in Cape Town and covers the Kinetheodolite setup at the then Royal Observatory and manned by part time amateur observers. It also covers the activities of Roberts at his Kenilworth tracking site. The activities of Bill Hirst and his station at Bergvliet are also described.

  13. Staircase and Fractional Part Functions

    ERIC Educational Resources Information Center

    Amram, Meirav; Dagan, Miriam; Ioshpe, Michael; Satianov, Pavel

    2016-01-01

    The staircase and fractional part functions are basic examples of real functions. They can be applied in several parts of mathematics, such as analysis, number theory, formulas for primes, and so on; in computer programming, the floor and ceiling functions are provided by a significant number of programming languages--they have some basic uses in…

  14. Scutellarin inhibits cytochrome P450 isoenzyme 1A2 (CYP1A2) in rats.

    PubMed

    Jian, Tun-Yu; He, Jian-Chang; He, Gong-Hao; Feng, En-Fu; Li, Hong-Liang; Bai, Min; Xu, Gui-Li

    2012-08-01

    Scutellarin is the most important flavone glycoside in the herbal drug Erigeron breviscapus (Vant.) Hand.-Mazz. It is used frequently in the clinic to treat ischemic vascular diseases in China. However, the direct relationship between scutellarin and cytochrome P450 (CYP450) is unclear. The present study investigated the in vitro and in vivo effects of scutellarin on cytochrome P450 1A2 (CYP 1A2) metabolism. According to in vitro experiments, scutellarin (10-250 µM) decreased the formation of 4-acetamidophenol in a concentration-dependent manner, with an IC₅₀ value of 108.20 ± 0.657 µM. Furthermore, scutellarin exhibited a weak mixed-type inhibition against the activity of CYP1A2 in rat liver microsomes, with a K(i) value of 95.2 µM. Whereas in whole animal studies, scutellarin treatment for 7 days (at 5, 15, 30 mg/kg, i.p.) decreased the clearance (CL), and increased the T(1/2) (at 15, 30 mg/kg, i.p.), it did not affect the V(d) of phenacetin. Scutellarin treatment (at 5, 15, 30 mg/kg, i.p.) increased the AUC(0-∞) by 14.3%, 67.3% and 159.2%, respectively. Scutellarin at 30 mg/kg also weakly inhibited CYP1A2 activity, in accordance with our in vitro study. Thus, the results indicate that CYP1A2 is inhibited directly, but weakly, by scutellarin in vivo, and provide useful information on the safe and effective use of scutellarin in clinical practice.

  15. 6. SITE OVERVIEW. PART 1 OF 6 PART PANORAMA WITH ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. SITE OVERVIEW. PART 1 OF 6 PART PANORAMA WITH NOS. CA-265-7 TO CA-265-11. ARROYO SECO PARKWAY AS SEEN FROM RADIO TOWER HILL (APPROXIMATELY 34° 5' BY 118° 12'30" ON USGS LOS ANGELES QUADRANGLE). PART 1 SHOWS GRAND VIEW POINT AT RIGHT REAR (LOCATION OF CAMERA POSITION FOR PHOTOGRAPHS NOS. 265-1 TO CA-265-5) AND FIGUEROA VIADUCT OVERCROSSING; DOWNTOWN LOS ANGELES IS AT LEFT REAR. LOOKING 234° SW. - Arroyo Seco Parkway, Los Angeles to Pasadena, Los Angeles, Los Angeles County, CA

  16. The MAJORANA Parts Tracking Database

    NASA Astrophysics Data System (ADS)

    Abgrall, N.; Aguayo, E.; Avignone, F. T.; Barabash, A. S.; Bertrand, F. E.; Brudanin, V.; Busch, M.; Byram, D.; Caldwell, A. S.; Chan, Y.-D.; Christofferson, C. D.; Combs, D. C.; Cuesta, C.; Detwiler, J. A.; Doe, P. J.; Efremenko, Yu.; Egorov, V.; Ejiri, H.; Elliott, S. R.; Esterline, J.; Fast, J. E.; Finnerty, P.; Fraenkle, F. M.; Galindo-Uribarri, A.; Giovanetti, G. K.; Goett, J.; Green, M. P.; Gruszko, J.; Guiseppe, V. E.; Gusev, K.; Hallin, A. L.; Hazama, R.; Hegai, A.; Henning, R.; Hoppe, E. W.; Howard, S.; Howe, M. A.; Keeter, K. J.; Kidd, M. F.; Kochetov, O.; Konovalov, S. I.; Kouzes, R. T.; LaFerriere, B. D.; Leon, J. Diaz; Leviner, L. E.; Loach, J. C.; MacMullin, J.; Martin, R. D.; Meijer, S. J.; Mertens, S.; Miller, M. L.; Mizouni, L.; Nomachi, M.; Orrell, J. L.; O`Shaughnessy, C.; Overman, N. R.; Petersburg, R.; Phillips, D. G.; Poon, A. W. P.; Pushkin, K.; Radford, D. C.; Rager, J.; Rielage, K.; Robertson, R. G. H.; Romero-Romero, E.; Ronquest, M. C.; Shanks, B.; Shima, T.; Shirchenko, M.; Snavely, K. J.; Snyder, N.; Soin, A.; Suriano, A. M.; Tedeschi, D.; Thompson, J.; Timkin, V.; Tornow, W.; Trimble, J. E.; Varner, R. L.; Vasilyev, S.; Vetter, K.; Vorren, K.; White, B. R.; Wilkerson, J. F.; Wiseman, C.; Xu, W.; Yakushev, E.; Young, A. R.; Yu, C.-H.; Yumatov, V.; Zhitnikov, I.

    2015-04-01

    The MAJORANA DEMONSTRATOR is an ultra-low background physics experiment searching for the neutrinoless double beta decay of 76Ge. The MAJORANA Parts Tracking Database is used to record the history of components used in the construction of the DEMONSTRATOR. The tracking implementation takes a novel approach based on the schema-free database technology CouchDB. Transportation, storage, and processes undergone by parts such as machining or cleaning are linked to part records. Tracking parts provide a great logistics benefit and an important quality assurance reference during construction. In addition, the location history of parts provides an estimate of their exposure to cosmic radiation. A web application for data entry and a radiation exposure calculator have been developed as tools for achieving the extreme radio-purity required for this rare decay search.

  17. The Majorana Parts Tracking Database

    SciTech Connect

    Abgrall, N.

    2015-01-16

    The Majorana Demonstrator is an ultra-low background physics experiment searching for the neutrinoless double beta decay of 76Ge. The Majorana Parts Tracking Database is used to record the history of components used in the construction of the Demonstrator. The tracking implementation takes a novel approach based on the schema-free database technology CouchDB. Transportation, storage, and processes undergone by parts such as machining or cleaning are linked to part records. Tracking parts provides a great logistics benefit and an important quality assurance reference during construction. In addition, the location history of parts provides an estimate of their exposure to cosmic radiation. In summary, a web application for data entry and a radiation exposure calculator have been developed as tools for achieving the extreme radio-purity required for this rare decay search.

  18. The Majorana Parts Tracking Database

    DOE PAGESBeta

    Abgrall, N.

    2015-01-16

    The Majorana Demonstrator is an ultra-low background physics experiment searching for the neutrinoless double beta decay of 76Ge. The Majorana Parts Tracking Database is used to record the history of components used in the construction of the Demonstrator. The tracking implementation takes a novel approach based on the schema-free database technology CouchDB. Transportation, storage, and processes undergone by parts such as machining or cleaning are linked to part records. Tracking parts provides a great logistics benefit and an important quality assurance reference during construction. In addition, the location history of parts provides an estimate of their exposure to cosmic radiation.more » In summary, a web application for data entry and a radiation exposure calculator have been developed as tools for achieving the extreme radio-purity required for this rare decay search.« less

  19. The Majorana Parts Tracking Database

    SciTech Connect

    Abgrall, N.; Aguayo, E.; Avignone, F. T.; Barabash, A. S.; Bertrand, F. E.; Brudanin, V.; Busch, M.; Byram, D.; Caldwell, A. S.; Chan, Y-D.; Christofferson, C. D.; Combs, D. C.; Cuesta, C.; Detwiler, J. A.; Doe, P. J.; Efremenko, Yu.; Egorov, V.; Ejiri, H.; Elliott, S. R.; Esterline, J.; Fast, J. E.; Finnerty, P.; Fraenkle, F. M.; Galindo-Uribarri, A.; Giovanetti, G. K.; Goett, J.; Green, M. P.; Gruszko, J.; Guiseppe, V. E.; Gusev, K.; Hallin, A. L.; Hazama, R.; Hegai, A.; Henning, R.; Hoppe, E. W.; Howard, S.; Howe, M. A.; Keeter, K. J.; Kidd, M. F.; Kochetov, O.; Konovalov, S. I.; Kouzes, R. T.; LaFerriere, B. D.; Leon, J. Diaz; Leviner, L. E.; Loach, J. C.; MacMullin, J.; Martin, R. D.; Meijer, S. J.; Mertens, S.; Miller, M. L.; Mizouni, L.; Nomachi, M.; Orrell, J. L.; O׳Shaughnessy, C.; Overman, N. R.; Petersburg, R.; Phillips, D. G.; Poon, A. W. P.; Pushkin, K.; Radford, D. C.; Rager, J.; Rielage, K.; Robertson, R. G. H.; Romero-Romero, E.; Ronquest, M. C.; Shanks, B.; Shima, T.; Shirchenko, M.; Snavely, K. J.; Snyder, N.; Soin, A.; Suriano, A. M.; Tedeschi, D.; Thompson, J.; Timkin, V.; Tornow, W.; Trimble, J. E.; Varner, R. L.; Vasilyev, S.; Vetter, K.; Vorren, K.; White, B. R.; Wilkerson, J. F.; Wiseman, C.; Xu, W.; Yakushev, E.; Young, A. R.; Yu, C. -H.; Yumatov, V.; Zhitnikov, I.

    2015-04-01

    The MAJORANA DEMONSTRATOR is an ultra-low background physics experiment searching for the neutrinoless double beta decay of 76Ge. The MAJORANA Parts Tracking Database is used to record the history of components used in the construction of the DEMONSTRATOR. Transportation, storage, and processes undergone by parts such as machining or cleaning are linked to part records. Tracking parts provides a great logistics benefit and an important quality assurance reference during construction. In addition, the location history of parts provides an estimate of their exposure to cosmic radiation. A web application for data entry and a radiation exposure calculator have been developed as tools for achieving the extreme radiopurity required for this rare decay search.

  20. GSFC Preferred Parts List PPL-15

    NASA Technical Reports Server (NTRS)

    Adolphsen, J. W. (Editor)

    1980-01-01

    A listing is presented of preferred electronic parts, part upgrading procedures, part derating guidelines, and part screening procedures to be used in the selection, procurement, and application of parts for Goddard Space Flight Center space systems and ground support equipment.

  1. GSFC preferred parts lists PPL-17

    NASA Technical Reports Server (NTRS)

    Baldini, B. P. (Editor)

    1984-01-01

    A listing of preferred parts, part upgrading procedures, part derating guidelines, and part screening procedures to be used in the selection, procurement, and application of parts for Goddard Space Flight Center (GSFC) space systems and ground support equipment is contained.

  2. The RASSF1A Tumor Suppressor Regulates XPA-Mediated DNA Repair

    PubMed Central

    Donninger, Howard; Clark, Jennifer; Rinaldo, Francesca; Nelson, Nicholas; Barnoud, Thibaut; Schmidt, M. Lee; Hobbing, Katharine R.; Vos, Michele D.; Sils, Brian

    2014-01-01

    RASSF1A may be the most frequently inactivated tumor suppressor identified in human cancer so far. It is a proapoptotic Ras effector and plays an important role in the apoptotic DNA damage response (DDR). We now show that in addition to DDR regulation, RASSF1A also plays a key role in the DNA repair process itself. We show that RASSF1A forms a DNA damage-regulated complex with the key DNA repair protein xeroderma pigmentosum A (XPA). XPA requires RASSF1A to exert full repair activity, and RASSF1A-deficient cells exhibit an impaired ability to repair DNA. Moreover, a cancer-associated RASSF1A single-nucleotide polymorphism (SNP) variant exhibits differential XPA binding and inhibits DNA repair. The interaction of XPA with other components of the repair complex, such as replication protein A (RPA), is controlled in part by a dynamic acetylation/deacetylation cycle. We found that RASSF1A and its SNP variant differentially regulate XPA protein acetylation, and the SNP variant hyperstabilizes the XPA-RPA70 complex. Thus, we identify two novel functions for RASSF1A in the control of DNA repair and protein acetylation. As RASSF1A modulates both apoptotic DDR and DNA repair, it may play an important and unanticipated role in coordinating the balance between repair and death after DNA damage. PMID:25368379

  3. Understanding the Multifaceted Role of Human Down Syndrome Kinase DYRK1A.

    PubMed

    Kay, L J; Smulders-Srinivasan, T K; Soundararajan, M

    2016-01-01

    The dual-specificity tyrosine (Y) phosphorylation-regulated kinase DYRK1A, also known as Down syndrome (DS) kinase, is a dosage-dependent signaling kinase that was originally shown to be highly expressed in DS patients as a consequence of trisomy 21. Although this was evident some time ago, it is only in recent investigations that the molecular roles of DYRK1A in a wide range of cellular processes are becoming increasingly apparent. Since initial knowledge on DYRK1A became evident through minibrain mnb, the Drosophila homolog of DYRK1A, this review will first summarize the scientific reports on minibrain and further expand on the well-established neuronal functions of mammalian and human DYRK1A. Recent investigations across the current decade have provided rather interesting and compelling evidence in establishing nonneuronal functions for DYRK1A, including its role in infection, immunity, cardiomyocyte biology, cancer, and cell cycle control. The latter part of this review will therefore focus in detail on the emerging nonneuronal functions of DYRK1A and summarize the regulatory role of DYRK1A in controlling Tau and α-synuclein. Finally, the emerging role of DYRK1A in Parkinson's disease will be outlined. PMID:27567487

  4. Cytochrome P450 1A1 Regulates Breast Cancer Cell Proliferation and Survival

    PubMed Central

    Rodriguez, Mariangellys; Potter, David A.

    2013-01-01

    Cytochrome P450 1A1 (CYP1A1) is an extrahepatic phase I metabolizing enzyme whose expression is suppressed under physiologic conditions, but can be induced by substrates via the aryl hydrocarbon receptor (AhR). Nonetheless, recent studies show that the majority of breast tumors constitutively express CYP1A1. These findings led us to test the hypothesis that CYP1A1 promotes breast cancer progression by evaluating the effects of CYP1A1 knock down on the proliferation and survival of the MCF7 and MDA-MB-231 lines. Independently of estrogen receptor status, CYP1A1 knock down decreases cell proliferation, decreases colony formation, blocks the cell cycle at G0/G1 associated with reduction of cyclin D1, and increases apoptosis associated with reduction of survivin. CYP1A1 knock down markedly increases phosphorylation of AMP-activated protein kinase (AMPK) and decreases phosphorylation of AKT, extracellular signal-regulated kinases 1 and 2 (ERK1/2), and 70kDa ribosomal protein S6 kinase (P70S6K). AMPK inhibition by compound C partially abrogates the pro-apoptotic effects of CYP1A1siRNA, suggesting that CYP1A1siRNA effects are mediated, in part, through AMPK signaling. Consistent with CYP1A1 knock down results, pharmacologic reduction of CYP1A1 levels by the phytopolyphenol carnosol also correlates with impaired proliferation and induced AMPK phosphorylation. These results indicate that reduction of basal CYP1A1 expression is critical for inhibition of proliferation, which is not affected by alpha-naphthoflavone-mediated inhibition of CYP1A1 activity nor modulated by AhR silencing. This study supports that CYP1A1 may promote breast cancer proliferation and survival, at least in part, through AMPK signaling and that reduction of CYP1A1 levels is a potential strategy for breast cancer therapeutics. PMID:23576571

  5. Polymorphisms of UGT1A1*6, UGT1A1*27 & UGT1A1*28 in three major ethnic groups from Malaysia

    PubMed Central

    Teh, L. K.; Hashim, H.; Zakaria, Z. A.; Salleh, M. Z.

    2012-01-01

    Background & objectives: Genetic polymorphisms of uridine diphosphate glucuronyltransferase 1A1 (UGT1A1) have been associated with a wide variation of responses among patients prescribed with irinotecan. Lack of this enzyme is known to be associated with a high incidence of severe toxicity. The objective of this study was to investigate the prevalence of three different variants of UGT1A1 (UGT1A1*6, UGT1A1*27 and UGT1A1*28), which are associated with reduced enzyme activity and increased irinotecan toxicity, in the three main ethnic groups in Malaysia (Malays, Chinese and Indians). Methods: A total of 306 healthy unrelated volunteers were screened for UGT1A1*28, UGT1A1*6 and UGT1A1*27. Blood samples (5 ml) were obtained from each subject and DNA was extracted. PCR based methods were designed and validated for detection of UGT1A1*6, UGT1A1*27 and UGT1A1*28. Direct DNA sequencing was performed to validate the results of randomly selected samples. Results: Malays and Indian have two-fold higher frequency of homozygous of UGT1A1*28 (7TA/7TA) which was 8 and 8.8 per cent, respectively compared to the Chinese (4.9%). However, the distribution of UGT1A1*6 and UGT1A1*27 showed no significant differences among them. UGT1A1*27 which has not been detected in Caucasian and African American population, was found in the Malaysian Malays (3.33%) and Malaysian Chinese (2.0%). Interpretation & conclusions: There was interethnic variability in the frequency of UGT1A1*28 in the Malaysian population. Our results suggest that genotyping of UGT1A1*6, UGT1A1*28 and UGT1A1*27 need to be performed before patients are prescribed with irinotecan due to their high prevalence of allelic variant which could lead to adverse drug reaction. PMID:22960892

  6. Aldehyde dehydrogenase 1A1 circumscribes high invasive glioma cells and predicts poor prognosis

    PubMed Central

    Xu, Sen-Lin; Liu, Sha; Cui, Wei; Shi, Yu; Liu, Qin; Duan, Jiang-Jie; Yu, Shi-Cang; Zhang, Xia; Cui, You-Hong; Kung, Hsiang-Fu; Bian, Xiu-Wu

    2015-01-01

    Glioma is the most aggressive brain tumor with high invasiveness and poor prognosis. More reliable, sensitive and practical biomarkers to reveal glioma high invasiveness remain to be explored for the guidance of therapy. We herein evaluated the diagnostic and prognostic value of aldehyde dehydrogenase 1A1 (ALDH1A1) in the glioma specimens from 237 patients, and found that ADLH1A1 was frequently overexpressed in the high-grade glioma (WHO grade III-IV) as compared to the low-grade glioma (WHO grade I-II) patients. The tumor cells with ALDH1A1 expression were more abundant in the region between tumor and the borderline of adjacent tissue as compared to the central part of the tumor. ALDH1A1 overexpression was associated with poor differentiation and dismal prognosis. Notably, the overall and disease-free survivals of the patients who had ALDH1A1+ tumor cells sparsely located in the adjacent tissue were much worse. Furthermore, ALDH1A1 expression was correlated with the “classical-like” (CL) subtype as we examined GBM specimens from 72 patients. Multivariate Cox regression analysis revealed that ALDH1A1 was an independent marker for glioma patients’ outcome. Mechanistically, both in vitro and in vivo studies revealed that ALDH1A1+ cells isolated from either a glioblastoma cell line U251 or primary glioblastoma cells displayed significant invasiveness, clonogenicity, and proliferation as compared to ALDH1A1- cells, due to increased levels of mRNA and protein for matrix metalloproteinase 2, 7 and 9 (MMP2, MMP7 and MMP9). These results indicate that ALDH1A1+ cells contribute to the progression of glioma including invasion, proliferation and poor prognosis, and suggest that targeting ALDH1A1 may have important implications for the treatment of highly invasive glioma. PMID:26101711

  7. Dimerization of human uridine diphosphate glucuronosyltransferase allozymes 1A1 and 1A9 alters their quercetin glucuronidation activities.

    PubMed

    Liu, Yan-Qing; Yuan, Ling-Min; Gao, Zhang-Zhao; Xiao, Yong-Sheng; Sun, Hong-Ying; Yu, Lu-Shan; Zeng, Su

    2016-01-01

    Uridine diphosphate glucuronosyltransferase 1A (UGT1A) is a major phase II drug-metabolism enzyme superfamily involved in the glucuronidation of endobiotics and xenobiotics in humans. Many polymorphisms in UGT1A genes are reported to inhibit or decrease UGT1A activity. In this study, two UGT1A1 allozymes, UGT1A1 wild-type and a splice mutant, as well as UGT1A9 wild-type and its three UGT1A9 allozymes, UGT1A9*2(C3Y), UGT1A9*3(M33T), and UGT1A9*5(D256N) were single- or double-expressed in a Bac-to-Bac expression system. Dimerization of UGT1A1 or UGT1A9 allozymes was observed via fluorescence resonance energy transfer (FRET) and co-immunoprecipitation analysis. SNPs of UGT1A altered the ability of protein-protein interaction, resulting in differential FRET efficiencies and donor-acceptor r distances. Dimerization changed the chemical regioselectivity, substrate-binding affinity, and enzymatic activity of UGT1A1 and UGT1A9 in glucuronidation of quercetin. These findings provide molecular insights into the consequences of homozygous and heterozygous UGT1A1 and UGT1A9 allozymes expression on quercetin glucuronidation. PMID:27025983

  8. Dimerization of human uridine diphosphate glucuronosyltransferase allozymes 1A1 and 1A9 alters their quercetin glucuronidation activities

    PubMed Central

    Liu, Yan-Qing; Yuan, Ling-Min; Gao, Zhang-Zhao; Xiao, Yong-Sheng; Sun, Hong-Ying; Yu, Lu-Shan; Zeng, Su

    2016-01-01

    Uridine diphosphate glucuronosyltransferase 1A (UGT1A) is a major phase II drug-metabolism enzyme superfamily involved in the glucuronidation of endobiotics and xenobiotics in humans. Many polymorphisms in UGT1A genes are reported to inhibit or decrease UGT1A activity. In this study, two UGT1A1 allozymes, UGT1A1 wild-type and a splice mutant, as well as UGT1A9 wild-type and its three UGT1A9 allozymes, UGT1A9*2(C3Y), UGT1A9*3(M33T), and UGT1A9*5(D256N) were single- or double-expressed in a Bac-to-Bac expression system. Dimerization of UGT1A1 or UGT1A9 allozymes was observed via fluorescence resonance energy transfer (FRET) and co-immunoprecipitation analysis. SNPs of UGT1A altered the ability of protein-protein interaction, resulting in differential FRET efficiencies and donor-acceptor r distances. Dimerization changed the chemical regioselectivity, substrate-binding affinity, and enzymatic activity of UGT1A1 and UGT1A9 in glucuronidation of quercetin. These findings provide molecular insights into the consequences of homozygous and heterozygous UGT1A1 and UGT1A9 allozymes expression on quercetin glucuronidation. PMID:27025983

  9. Catalog of Infrared Observations. Part I: Data. Part II: Appendixes.

    NASA Astrophysics Data System (ADS)

    Gezari, D. Y.; Schmitz, M.; Mead, J. M.

    The Catalog of Infrared Observations (CIO) is a compilation of infrared astronomical data obtained from an extensive literature search of astronomical journals and major astronomical catalogs and surveys. The literature searches are complete for years 1965 through 1986. The Catalog is published in two parts, with the observational data (roughly 200,000 observations of 20,000 individual sources) listed in Part I, and supporting appendices in Part II. The expanded Second Edition contains a new feature: complete IRAS 4-band data for all CIO sources detected, listed with the main Catalog observations, as well as in complete detail in the Appendix. The appendices include an atlas of infrared source positions, two bibliographies of infrared literature upon which the search was based, and an atlas of infrared spectral ranges, and IRAS data for the CIO sources. The complete CIO database is available in printed microfiche and magnetic tape formats.

  10. Indentured Parts List Maintenance and Part Assembly Capture Tool - IMPACT

    NASA Technical Reports Server (NTRS)

    Jain, Bobby; Morris, Jill; Sharpe, Kelly

    2004-01-01

    Johnson Space Center's (JSC's) indentured parts list (IPL) maintenance and parts assembly capture tool (IMPACT) is an easy-to-use graphical interface for viewing and maintaining the complex assembly hierarchies of large databases. IMPACT, already in use at JSC to support the International Space Station (ISS), queries, updates, modifies, and views data in IPL and associated resource data, functions that it can also perform, with modification, for any large commercial database. By enabling its users to efficiently view and manipulate IPL hierarchical data, IMPACT performs a function unlike that of any other tool. Through IMPACT, users will achieve results quickly, efficiently, and cost effectively.

  11. 45 CFR 630.105 - Does this part apply to me?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Does this part apply to me? 630.105 Section 630.105 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION... this part apply to me? (a) Portions of this part apply to you if you are either— (1) A recipient of...

  12. 10 CFR 708.2 - What are the definitions of terms used in this part?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false What are the definitions of terms used in this part? 708.2... § 708.2 What are the definitions of terms used in this part? For purposes of this part: Contractor means a seller of goods or services who is a party to: (1) A management and operating contract or...

  13. 40 CFR Table 1 to Subpart Nnn of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart NNN

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CFR Part 63, Subpart A) to Subpart NNN 1 Table 1 to Subpart NNN of Part 63 Protection of Environment... General Provisions (40 CFR Part 63, Subpart A) to Subpart NNN General provisions citation Requirement Applies tosubpart NNN Explanation 63.1(a)(1)-(a)(4) Applicability Yes. 63.1(a)(5) No ....

  14. Cell cycle restriction is more important than apoptosis induction for RASSF1A protein tumor suppression.

    PubMed

    Donninger, Howard; Clark, Jennifer A; Monaghan, Megan K; Schmidt, M Lee; Vos, Michele; Clark, Geoffrey J

    2014-11-01

    The Ras association domain family protein 1A (RASSF1A) is arguably one of the most frequently inactivated tumor suppressors in human cancer. RASSF1A modulates apoptosis via the Hippo and Bax pathways but also modulates the cell cycle. In part, cell cycle regulation appears to be dependent upon the ability of RASSF1A to complex with microtubules and regulate their dynamics. Which property of RASSF1A, apoptosis induction or microtubule regulation, is responsible for its tumor suppressor function is not known. We have identified a short conserved motif that is essential for the binding of RASSF family proteins with microtubule-associated proteins. By making a single point mutation in the motif, we were able to generate a RASSF1A variant that retains wild-type apoptotic properties but completely loses the ability to bind microtubule-associated proteins and complex with microtubules. Comparison of this mutant to wild-type RASSF1A showed that, despite retaining its proapoptotic properties, the mutant was completely unable to induce cell cycle arrest or suppress the tumorigenic phenotype. Therefore, it appears that the cell cycle/microtubule effects of RASSF1A are key to its tumor suppressor function rather than its apoptotic effects.

  15. Electronic spectroscopy of the A1A'' <-- X1A' system of CDF.

    PubMed

    Tao, Chong; Deselnicu, Mihaela; Fan, Haiyan; Mukarakate, Calvin; Ionescu, Ionela; Reid, Scott A

    2006-02-14

    To further investigate the Renner-Teller (RT) effect and barriers to linearity and dissociation in the simplest singlet carbene, we recorded fluorescence excitation spectra of bands involving the pure bending levels 2(n)(0) with n = 0-9 and the combination states 1(1)(0)2(n)(0) with n = 1-8 and 2(n)(0)3(1)(0) with n = 0-5 in the A(1)A''<-- X(1)A' system of CDF, in addition to some weak hot bands. The spectra were measured under jet-cooled conditions using a pulsed discharge source, and rotationally analyzed to yield precise values for the band origins and rotational constants; fluorescence lifetimes were also measured to probe for lifetime lengthening effects due to the RT interaction. The derived A state parameters are compared with previous results for CHF and with predictions of ab initio electronic structure theory. The approach to linearity in the A state is evidenced in a sharp increase in the A rotational constant with bending excitation, and a minimum in the vibrational intervals near 2(9). A fit of the vibrational intervals for the pure bending levels yields an A state barrier to linearity in good agreement both with that previously derived for CHF and ab initio predictions. From the spectra and lifetime measurements, the onset of extensive RT perturbations is found to occur at a higher energy than in CHF, consistent with the smaller A constant.

  16. MISR Level 1A CCD Science data, all cameras (MIL1A_V2)

    NASA Technical Reports Server (NTRS)

    Diner, David J. (Principal Investigator)

    The Level 1A data are raw MISR data that are decommutated, reformatted 12-bit Level 0 data shifted to byte boundaries, i.e., reversal of square-root encoding applied and converted to 16 bit, and annotated (e.g., with time information). These data are used by the Level 1B1 processing algorithm to generate calibrated radiances. The science data output preserves the spatial sampling rate of the Level 0 raw MISR CCD science data. CCD data are collected during routine science observations of the sunlit portion of the Earth. Each product represents one 'granule' of data. A 'granule' is defined to be the smallest unit of data required for MISR processing. Also, included in the Level 1A product are pointers to calibration coefficient files provided for Level 1B processing. [Location=GLOBAL] [Temporal_Coverage: Start_Date=2000-02-24; Stop_Date=] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180].

  17. MISR Level 1A CCD Science data, all cameras (MIL1A_V1)

    NASA Technical Reports Server (NTRS)

    Diner, David J. (Principal Investigator)

    The Level 1A data are raw MISR data that are decommutated, reformatted 12-bit Level 0 data shifted to byte boundaries, i.e., reversal of square-root encoding applied and converted to 16 bit, and annotated (e.g., with time information). These data are used by the Level 1B1 processing algorithm to generate calibrated radiances. The science data output preserves the spatial sampling rate of the Level 0 raw MISR CCD science data. CCD data are collected during routine science observations of the sunlit portion of the Earth. Each product represents one 'granule' of data. A 'granule' is defined to be the smallest unit of data required for MISR processing. Also, included in the Level 1A product are pointers to calibration coefficient files provided for Level 1B processing. [Location=GLOBAL] [Temporal_Coverage: Start_Date=2000-02-24; Stop_Date=] [Spatial_Coverage: Southernmost_Latitude=-90; Northernmost_Latitude=90; Westernmost_Longitude=-180; Easternmost_Longitude=180].

  18. Formation of parting in quartz

    NASA Astrophysics Data System (ADS)

    Jørgen Kjøll, Hans; Eske Sørensen, Bjørn

    2014-05-01

    This paper presents hydrothermal quartz with macroscopic planar parting from the Mesoproterozoic Modum complex in southern Norway. Similar macroscopic parting in hydrothermal quartz with macroscopic planar structures has only been described from two localities in the world; Madagascar (Flörke et al., 1981) and Southern California (Murdoch et al., 1938). The study area consists of well foliated and banded sillimanite- garnet- amphibolite- mica gneiss that is cut at high angle by hydrothermal veins containing albite, chlinoclore, hornblende, hydroxyl apatite and quartz. The rim of the veins is generally made up of almost pure end-member euhedral albite. Then there is vugs with euhedral hornblende (10-25cm long) and euhedral hydroxyl apatite with size ranging from mm scale to several cm. Some places the quartz encloses apatite and hornblende. The quartz is anhedral, inequigranular with undulose extinction bordering sub grain rotation. It has large planar penetrative parting faces with pearly luster; however this is not consistent throughout the outcrop and some places the penetrative faces disappears and the quartz has a conchoidal fracture. The planar faces continue throughout the specimens with a few mm spacing. Thin sections oriented perpendicular to the most pronounced planar structure show lamellas that extinguishes at small angles (2 degrees) to each other. EBSD mapping of the planar faces shows two orientations {0-111} and {1-101}, corresponding to the r- and z-faces respectively, separated by irregular boundaries. The misorientation between these two crystallographic orientations on the parting is a 60 degree rotation on [0 0 1] in correspondence to the dauphiné twin law. Investigations conducted on thin sections cut orthogonal to the parting shows that the parting cuts and offsets the dauphiné twins, indicating a late genesis of the parting. However some internal stress induced movement of the twins are visible. SEM-CL documents three generations of quartz

  19. Staircase and fractional part functions

    NASA Astrophysics Data System (ADS)

    Amram, Meirav; Dagan, Miriam; Ioshpe, Michael; Satianov, Pavel

    2016-10-01

    The staircase and fractional part functions are basic examples of real functions. They can be applied in several parts of mathematics, such as analysis, number theory, formulas for primes, and so on; in computer programming, the floor and ceiling functions are provided by a significant number of programming languages - they have some basic uses in various programming tasks. In this paper, we view the staircase and fractional part functions as a classical example of non-continuous real functions. We introduce some of their basic properties, present some interesting constructions concerning them, and explore some intriguing interpretations of such functions. Throughout the paper, we use these functions in order to explain basic concepts in a first calculus course, such as domain of definition, discontinuity, and oddness of functions. We also explain in detail how, after researching the properties of such functions, one can draw their graph; this is a crucial part in the process of understanding their nature. In the paper, we present some subjects that the first-year student in the exact sciences may not encounter. We try to clarify those subjects and show that such ideas are important in the understanding of non-continuous functions, as a part of studying analysis in general.

  20. Calculus: A Computer Oriented Presentation, Part 1 [and] Part 2.

    ERIC Educational Resources Information Center

    Stenberg, Warren; Walker, Robert J.

    Parts one and two of a one-year computer-oriented calculus course (without analytic geometry) are presented. The ideas of calculus are introduced and motivated through computer (i.e., algorithmic) concepts. An introduction to computing via algorithms and a simple flow chart language allows the book to be self-contained, except that material on…

  1. Border Issues in Education, Part 1 [and] Part 2.

    ERIC Educational Resources Information Center

    Alexander-Kasparik, Rosalind, Ed.; Soulas, John, Comp.

    1994-01-01

    These newsletters examine issues in education along the United States and Mexico border. Topics in Part 1 include the ramifications of the North American Free Trade Agreement (NAFTA) for education, the impact of immigration on schools, and the structure and history of the Mexican educational system and its reforms in theory and practice. Educators…

  2. X-pinch. Part II

    SciTech Connect

    Pikuz, S. A. Shelkovenko, T. A.; Hammer, D. A.

    2015-06-15

    Results of experimental studies of the X-pinch since its invention and implementation in 1982 at the Lebedev Physical Institute are presented. The review consists of two parts. The first part briefly outlines the history of creation and studies of X-pinches, describes the diagnostic techniques and devices developed during these studies, and presents the main results obtained in studying the physical processes occurring in the X-pinch. The second part is devoted to the results of detailed studies of the spatial, temporal, and spectral characteristics of the X-pinch hot spot—the region where the highest plasma parameters are achieved and which is a source of X-ray emission with extreme parameters. Some results of X-pinch simulations are also presented.

  3. Surface Seal for Carbon Parts

    NASA Technical Reports Server (NTRS)

    Shuford, D. M.; Spruiell, J. P.

    1982-01-01

    Surface pores in parts made of graphite or reinforced-carbon/ carbon materials are sealed by a silicon carbide-based coating. Coating inhibits subsurface oxidation and lengthens part life. Starting material for coating is graphite felt, which is converted to silicon carbide felt by processing it according to a prescribed time/temperature schedule. Converted felt is pulverized in a ball mill and resulting powder is mixed with an equal weight of black silicon carbide powder. Powder mixture is combined with an equal weight of adhesive to form a paste.

  4. Grinding Parts For Automatic Welding

    NASA Technical Reports Server (NTRS)

    Burley, Richard K.; Hoult, William S.

    1989-01-01

    Rollers guide grinding tool along prospective welding path. Skatelike fixture holds rotary grinder or file for machining large-diameter rings or ring segments in preparation for welding. Operator grasps handles to push rolling fixture along part. Rollers maintain precise dimensional relationship so grinding wheel cuts precise depth. Fixture-mounted grinder machines surface to quality sufficient for automatic welding; manual welding with attendant variations and distortion not necessary. Developed to enable automatic welding of parts, manual welding of which resulted in weld bead permeated with microscopic fissures.

  5. Understanding quantitative research: part 1.

    PubMed

    Hoe, Juanita; Hoare, Zoë

    This article, which is the first in a two-part series, provides an introduction to understanding quantitative research, basic statistics and terminology used in research articles. Critical appraisal of research articles is essential to ensure that nurses remain up to date with evidence-based practice to provide consistent and high-quality nursing care. This article focuses on developing critical appraisal skills and understanding the use and implications of different quantitative approaches to research. Part two of this article will focus on explaining common statistical terms and the presentation of statistical data in quantitative research.

  6. The Ã1A'- X˜1A' single vibronic level fluorescence spectrum of styrene vapor

    NASA Astrophysics Data System (ADS)

    Hollas, J. Michael; Ridley, Trevor

    1981-09-01

    Single vibronic level (SVL) fluorescence spectra following excitation into the 0 00 band and many sequence and cross-sequence bands in the Ã1A'- X˜1A' system of styrene have been recorded and assigned. The technique is shown to be very powerful in obtaining accurate energy levels for low-wavenumber vibrations, particularly in the X˜ state, where it is capable of giving information regarding vibrational levels inaccessible by conventional infrared or Raman spectroscopy. Interpretation of the spectra leads to many new assignments in the absorption spectrum and to an accurate knowledge of many vibrational energy levels. An important reassignment is that of the 41 0142 01 band previously assigned to 40 02 ( ν40, ν41, and ν42 are all a″ vibrations). The two most important pieces of information which derive from analysis of the SVL spectra are (a) the first five vibrational levels of ν42, the C(1)- C( α) torsional vibration, in the X˜ state, each accurate to about ±0.2 cm -1, and (b) the fact that the normal coordinates of ν42 and ν41, an out-of-plane substituent vibration, are very heavily mixed in the Ã, relative to the X˜, state—an extreme case of the Duschinsky effect. As a result of analysis of the SVL spectra, analysis of the absorption spectrum is now so complete that we can be confident that the Hui and Rice proposal that the CH 2 group of the substituent is perpendicular to the plane of the rest of the molecule in the à state has no evidence to support it.

  7. Parts Specialist. Teacher Edition. Second Edition.

    ERIC Educational Resources Information Center

    Fuqua, Lou; And Others

    This set of instructional materials provides secondary and postsecondary students with an opportunity to explore the career choices available in the parts merchandising industry. The skills presented are useful to any student pursuing a career in auto parts, heavy equipment parts, small engine parts, tractor parts, appliance parts, or for any…

  8. 40 CFR Appendix: Table 2 to... - Applicability of 40 CFR Part 63 General Provisions to Subpart HHH

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 11 2010-07-01 2010-07-01 true Applicability of 40 CFR Part 63 General... HHH of Part 63—Applicability of 40 CFR Part 63 General Provisions to Subpart HHH General provisions reference Applicable tosubpart HHH Explanation § 63.1(a)(1) Yes § 63.1(a)(2) Yes § 63.1(a)(3) Yes §...

  9. Air Pollution Control, Part I.

    ERIC Educational Resources Information Center

    Strauss, Werner, Ed.

    Authoritative reviews in seven areas of current importance in air pollution control are supplied in this volume, the first of a two-part set. Titles contained in this book are: "Dispersion of Pollutants Emitted into the Atmosphere,""The Formation and Control of Oxides of Nitrogen in Air Pollution,""The Control of Sulfur Emissions from Combustion…

  10. Taking Part One GNVQ Forward.

    ERIC Educational Resources Information Center

    Frankland, Gillian; Ebrahim, Nazir

    This document is intended to assist practitioners involved in the delivery of Part One General National Vocational Qualifications (GNVQs), which are GNVQs designed for students in the United Kingdom who are at Key Stage 4 and the post-16 level. Section 1 examines the following topics of interest to centers that are contemplating introducing Part…

  11. Machine Shop Fundamentals: Part I.

    ERIC Educational Resources Information Center

    Kelly, Michael G.; And Others

    These instructional materials were developed and designed for secondary and adult limited English proficient students enrolled in machine tool technology courses. Part 1 includes 24 lessons covering introduction, safety and shop rules, basic machine tools, basic machine operations, measurement, basic blueprint reading, layout, and bench tools.…

  12. List of Preferred Electronic Parts

    NASA Technical Reports Server (NTRS)

    Covey, R. E.; Scott, W. R.; Hess, L. M.; Steffy, G.; Stott, F. R.

    1986-01-01

    Components passed stringent qualification tests tabulated and described. Destructive and nondestructive qualification testing confirmed parts capable of withstanding spacecraft environments without excessive degradation and have stable characteristics during operating lives. Testing assessed effects of electrical, environmental, and mechanical stresses in various levels and time durations.

  13. Glues Used in Airplane Parts

    NASA Technical Reports Server (NTRS)

    Allen, S W; Truax, T R

    1920-01-01

    This report was prepared for the National Advisory Committee for Aeronautics and presents the results of investigations conducted by the Forest Products Laboratory of the United States Forest Service on the manufacture, preparation, application, testing and physical properties of the different types of glues used in wood airplane parts.

  14. Speech parts as Poisson processes.

    PubMed

    Badalamenti, A F

    2001-09-01

    This paper presents evidence that six of the seven parts of speech occur in written text as Poisson processes, simple or recurring. The six major parts are nouns, verbs, adjectives, adverbs, prepositions, and conjunctions, with the interjection occurring too infrequently to support a model. The data consist of more than the first 5000 words of works by four major authors coded to label the parts of speech, as well as periods (sentence terminators). Sentence length is measured via the period and found to be normally distributed with no stochastic model identified for its occurrence. The models for all six speech parts but the noun significantly distinguish some pairs of authors and likewise for the joint use of all words types. Any one author is significantly distinguished from any other by at least one word type and sentence length very significantly distinguishes each from all others. The variety of word type use, measured by Shannon entropy, builds to about 90% of its maximum possible value. The rate constants for nouns are close to the fractions of maximum entropy achieved. This finding together with the stochastic models and the relations among them suggest that the noun may be a primitive organizer of written text.

  15. Part-Time Faculty Handbook.

    ERIC Educational Resources Information Center

    Hillsborough Community Coll., Tampa, FL.

    Policies, regulations, procedures, and general information are provided in this handbook designed to help orient new part-time faculty at Hillsborough Community College (HCC). After introductory material outlining the history of HCC and stressing the importance of the student in HCC's mission, the handbook describes HCC's evening and weekend…

  16. Playing It Safe: Part II.

    ERIC Educational Resources Information Center

    Penman, Kenneth A.; Niccolai, Frances R.

    1985-01-01

    Explains how to prevent outdoor sports injuries; discusses related litigation and specific cases involving playing field turf, tennis, skiing, and pools; and sets out facility design and maintenance considerations and recommendations. A sidebar provides information about injury insurance available to NCAA schools. Part I of this article appeared…

  17. Piecing Together a College Education behind Bars. Part One [and] Part Two [and] Part Three [and] Part Four.

    ERIC Educational Resources Information Center

    Taylor, Jon Marc

    2002-01-01

    This is a four-part article on how to earn a college degree from inside prison. The author, who earned his master's while in prison, argues that education offers prisoners the best chance of staying out of prison after their release, as well as qualifying them for a decent job and easing social reintegration. The author outlines the approaches…

  18. Laserdisk Directory--Part 3.

    ERIC Educational Resources Information Center

    Connolly, Bruce

    1986-01-01

    Presents third installment of directory published in four parts in June and August issues of "Database" and July and September "Online." Description, company, compatibility, type of laserdisk, search and retrieval software, interface with magnetic media, conditions of usage, price, and status information are given for 11 products. Company names…

  19. Laserdisk Directory--Part 2.

    ERIC Educational Resources Information Center

    Connolly, Bruce

    1986-01-01

    Second installment of directory published in four parts in "Database" (June and August) and "Online" (July and September) provides descriptions of 17 laserdisk products together with information on the producer, compatibility, type of laserdisk, search and retrieval software, interface with magnetic media, conditions of usage, price, and status.…

  20. Laserdisk Directory--Part 1.

    ERIC Educational Resources Information Center

    Connolly, Bruce, Comp.

    1986-01-01

    This first installment of four-part "Online/Database Laserdisk Directory" reports on aspects of laserdisks including: product name; product description; company name; conpatibility information; type of laserdisk (compact disc read-only-memory, videodisk); software used; interface with magnetic media capability; conditions of usage; price;…

  1. Laserdisk Directory--Part 5.

    ERIC Educational Resources Information Center

    Connolly, Bruce

    1986-01-01

    This continuation of the previously published four part directory reports information on several additional laserdisk databases and provides updates on some of those included in the original listing. Included in the product descriptions are producers; compatibility; type of laserdisk; software; interface with magnetic media; conditions of usage;…

  2. OCCUPATIONS FOR YOU, PART ONE.

    ERIC Educational Resources Information Center

    DES ROCHES, DEAN L.

    DEVELOPED AS PART OF A CURRICULUM PROJECT DESCRIBED IN VT 004 454 TO HELP YOUNG PEOPLE LEARN BASIC PRINCIPLES AND CONCEPTS OF MECHANICS AND TECHNOLOGY, THIS BOOK WAS DESIGNED TO STIMULATE INTEREST AND ANSWER QUESTIONS ABOUT VOCATIONAL CHOICE, STIMULATE INTEREST IN READING, AND PROVIDE CONTENT TO RELATE TO ACADEMIC STUDY. THE MATERIALS DEVELOPED IN…

  3. Hydroforming of flanged tubular part

    SciTech Connect

    Joo, B. D.; Jang, J. H.; Choi, M. K.; Moon, Y. H.

    2010-06-15

    Tube hydroforming is the technology that utilizes hydraulic pressure to form a tube into desired shapes inside die cavities. Recently, tube hydroforming technology draws attentions of automotive industries due to its advantages such as weight reduction, increased strength, improved quality and reduced tooling cost. Hydroformed automotive parts used as structural components in vehicle body frame or subframe often have to be structurally joined at some point. Therefore it is useful if the hydroformed automotive parts can be given a localized attachment flange. In this study, a tube hydroformed product which has flange has been formed at various processing conditions. To accomplish successful flange hydroforming process, thorough investigation on proper combination of process parameters such as internal hydraulic pressure and tool geometry has been performed. For the process design FE analysis was performed with Dynaform 5.5. With optimized die parting angle and circumferential expansion ratio, hydroforming experiments to form flange were performed and forming characteristics at various process conditions were analyzed. The results show that flanged parts can be successfully produced by tube hydroforming process.

  4. Laserdisk Directory--Part 4.

    ERIC Educational Resources Information Center

    Connolly, Bruce

    1986-01-01

    Fourth installment of a directory published in four parts in "Database" (June and August) and "Online" (July and September) provides descriptions of 12 laserdisk products together with information on the producer, compatibility, type of laserdisk, search and retrieval software, interface with magnetic media, conditions of usage, price, and status.…

  5. Death: A Part of Life.

    ERIC Educational Resources Information Center

    Otero, George G.; Harris, Zoanne

    This two-part curriculum unit includes 20 slides depicting Days of the Dead in Mexico and the United States. The unit is designed to help middle school students compare customs and practices associated with death throughout the world in a way that promotes understanding of the values and needs that produce and are reinforced by death rituals and…

  6. Fabrication of polyetheretherketone (PEEK) parts

    SciTech Connect

    Nelson, R.W.

    1991-12-01

    A literature search was conducted for polyetheretherketone (PEEK) thermoplastic to investigate its physical properties and molding applications. Simple molds were designed, built, and tested using PEEK molding compound. Molding parameters and shrink factors were determined. Physical properties varied with the percent of crystalline PEEK present. Annealing the molded parts enhanced their physical properties and increased the percent crystallinity. 11 refs.

  7. The PC Connection Part III.

    ERIC Educational Resources Information Center

    Chu, David L.; Zilora, Karen S.

    1986-01-01

    Presents a series of four applications of data acquisition, system control, and data analysis using personal computers. Covers topics of pilot plant information and control, automation of drug safety evaluation, analysis and characterization of petroleum resources, and high-speed analog-digital conversion connections. Part three of a series on…

  8. Local Area Networks: Part II.

    ERIC Educational Resources Information Center

    Dessy, Raymond E., Ed.

    1982-01-01

    Discusses five approaches used by industry/colleges to provide local area network (LAN) capabilities in the analytical laboratory: (1) mixed baseband bus network coupled to a star net; (2) broadband bus network; (3) ring network; (4) star network coupled to broadband net; and (5) simple multiprocessor center. Part I (September issue) focused on…

  9. Part-Time Faculty Handbook.

    ERIC Educational Resources Information Center

    College of the Mainland, Texas City, TX.

    This document contains comprehensive information designed to orient the part-time faculty member to the College of the Mainland. Information included in the handbook covers the history and philosophy of the college; student and instructor rights; a description of social and economic characteristics of the students; a description of the faculty;…

  10. Avermectin B sub 1a metabolism in celery: A residue study

    SciTech Connect

    Moye, H.A.; Malagodi, M.H.; Yoh, Jau; Deyrup, C.L.; Chang Shou Mei; Leibee, G.L. ); Ku, C.C.; Wislocki, P.G. )

    1990-01-01

    Radioactivity equivalent to less than 4% of the total avermectin B{sub 1a} applied to mature and immature celery as a mixture of unlabeled and ({sup 14}C)- or ({sup 3}H) avermectin B{sub 1a} remained in harvested plants. The half-lives of radiolabeled avermectin B{sub 1a} residues as measured by the dissipation of total {sup 3}H radioactivity from celery parts ranged from 5.2 to 12.9 days in celery treated at 0.01 and 0.10 lb of AI/acre. The percentage of total radioactivity in celery parts readily extractable with acetone (range of 57.8-97.1%) generally decreased with increasing postharvest intervals. High-performance liquid chromatography of acetone extracts of celery leaves and stalks produced four discernible peaks of radioactivity, designated as polar metabolites, moderately polar metabolites, avermectin B{sub 1a}, and the {Delta}{sup 8,9} isomer of avermectin B{sub 1a}. The percentage of acetone extract radioactivity represented by polar metabolites generally increased with increasing postharvest intervals.

  11. Cleaning of parts for new manufacturing and parts rebuilding

    NASA Astrophysics Data System (ADS)

    Doherty, Jeff

    1994-06-01

    Parts cleaning is the largest single expense, and the most time consuming activity, in rebuilding and new manufacturing. On average, 25% to 40% of the total labor and overhead burden is spent on cleaning. EPA and OSHA pressures add to the burden by making some methods and chemicals obsolete. Some of the processes and chemicals in current use will be curtailed and or outlawed in the future. How can a shops and industries make long term decisions or capital investments in cleaning and process improvements when the government keeps changing its rules? At the MART Corporation in Saint Louis, Missouri, we manufacture a line of cabinet-style batch cleaning machines known as Power Washers. Twenty years ago MART invented and patented the Power Washer process, a cleaning method that recycles wash solution and blasts contaminates as they are washed off the more heavily contaminated parts. Since the initial invention MART has continued to R&D the washing process and develop ancillary systems that comply with EPA and OSHA regulations. For applications involving new industrial parts or items requiring specification cleaned surfaces. MART provides filtration and solution conditioning systems, part drying operations, and triple rinsing. Units are available in stainless steel or higher alloys. We are not alone in the washer manufacturing business. You have many choices of cleaning solutions (no pun intended) which will perform in your operations and yield good results. As a manufacturer, we are interested in your success with our equipment. We have all heard the horror stories of companies having selected inappropriate cleaning systems and or processes which then brought the company to its knees, production wise. Assembly, appearance, warranty, and performance shortcomings of finished products can often be directly related to the cleaning process and its shortcomings.

  12. Getting in Taped, Part I and Part II

    ERIC Educational Resources Information Center

    Cundy, H. M.; Higgins, J.

    1971-01-01

    This article is in two parts: discussion of mathematical concepts involved in converting the reading from the tape-recorder counter which counts the turns of the run-off spool to that from the counter which counts turns of the take-up spool; calculating the length of tape run off when given the reading from the tape-recorder counter of the run-off…

  13. Distinct enhancers of ptf1a mediate specification and expansion of ventral pancreas in zebrafish.

    PubMed

    Pashos, Evanthia; Park, Joon Tae; Leach, Steven; Fisher, Shannon

    2013-09-15

    Development of the pancreas and cerebellum require Pancreas-specific transcription factor-1a (Ptf1a), which encodes a subunit of the transcription factor complex PTF1. Ptf1a is required in succession for specification of the pancreas, proper allocation of pancreatic progenitors to endocrine and exocrine fates, and the production of digestive enzymes from the exocrine acini. In several neuronal structures, including the cerebellum, hindbrain, retina and spinal cord, Ptf1a is transiently expressed and promotes inhibitory neuron fates at the expense of excitatory fates. Transcription of Ptf1a in mouse is maintained in part by PTF1 acting on an upstream autoregulatory enhancer. However, the transcription factors and enhancers that initially activate Ptf1a expression in the pancreas and in certain structures of the nervous system have not yet been identified. Here we describe a zebrafish autoregulatory element, conserved among teleosts, with activity similar to that described in mouse. In addition, we performed a comprehensive survey of all non-coding sequences in a 67kb interval encompassing zebrafish ptf1a, and identified several neuronal enhancers, and an enhancer active in the ventral pancreas prior to activation of the autoregulatory enhancer. To test the requirement for autoregulatory control during pancreatic development, we restored ptf1a function through BAC transgenesis in ptf1a morphants, either with an intact BAC or one lacking the autoregulatory enhancer. We find that ptf1a autoregulation is required for development of the exocrine pancreas and full rescue of the ptf1a morphant phenotype. Similarly, we demonstrate that a ptf1a locus lacking the early enhancer region is also capable of rescue, but only supports formation of a hypoplastic exocrine pancreas. Through our dissection of the complex regulatory control of ptf1a, we identified separate cis-regulatory elements that underlie different aspects of its expression and function, and further demonstrated

  14. The Chromatin Remodeling Component Arid1a Is a Suppressor of Spontaneous Mammary Tumors in Mice.

    PubMed

    Kartha, Nithya; Shen, Lishuang; Maskin, Carolyn; Wallace, Marsha; Schimenti, John C

    2016-08-01

    Human cancer genome studies have identified the SWI/SNF chromatin remodeling complex member ARID1A as one of the most frequently altered genes in several tumor types. Its role as an ovarian tumor suppressor has been supported in compound knockout mice. Here, we provide genetic and functional evidence that Arid1a is a bona fide mammary tumor suppressor, using the Chromosome aberrations occurring spontaneously 3 (Chaos3) mouse model of sporadic breast cancer. About 70% of mammary tumors that formed in these mice contained a spontaneous deletion removing all or part of one Arid1a allele. Restoration of Arid1a expression in a Chaos3 mammary tumor line with low Arid1a levels greatly impaired its ability to form tumors following injection into cleared mammary glands, indicating that ARID1A insufficiency is crucial for maintenance of these Trp53-proficient tumors. Transcriptome analysis of tumor cells before and after reintroduction of Arid1a expression revealed alterations in growth signaling and cell-cycle checkpoint pathways, in particular the activation of the TRP53 pathway. Consistent with the latter, Arid1a reexpression in tumor cells led to increased p21 (Cdkn1a) expression and dramatic accumulation of cells in G2 phase of the cell cycle. These results not only provide in vivo evidence for a tumor suppressive and/or maintenance role in breast cancer, but also indicate a potential opportunity for therapeutic intervention in ARID1A-deficient human breast cancer subtypes that retain one intact copy of the gene and also maintain wild-type TRP53 activity. PMID:27280691

  15. 26 CFR 1.148-1A - Definitions and elections.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 26 Internal Revenue 2 2013-04-01 2013-04-01 false Definitions and elections. 1.148-1A Section 1.148-1A Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX....148-1A Definitions and elections. (a) . For guidance see § 1.148-1. (b) Certain...

  16. 26 CFR 1.148-1A - Definitions and elections.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 2 2012-04-01 2012-04-01 false Definitions and elections. 1.148-1A Section 1.148-1A Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX....148-1A Definitions and elections. (a) . For guidance see § 1.148-1. (b) Certain...

  17. 26 CFR 1.148-1A - Definitions and elections.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 2 2011-04-01 2011-04-01 false Definitions and elections. 1.148-1A Section 1.148-1A Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX....148-1A Definitions and elections. (a) . For guidance see § 1.148-1. (b) Certain...

  18. 26 CFR 1.148-1A - Definitions and elections.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 2 2010-04-01 2010-04-01 false Definitions and elections. 1.148-1A Section 1.148-1A Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX....148-1A Definitions and elections. (a) . For guidance see § 1.148-1. (b) Certain...

  19. 7 CFR 1a.5 - Responsibility of the Inspector General.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Responsibility of the Inspector General. 1a.5 Section 1a.5 Agriculture Office of the Secretary of Agriculture LAW ENFORCEMENT AUTHORITIES § 1a.5 Responsibility of the Inspector General. The Inspector General shall: (a) Issue directives conforming to...

  20. [UGT1A1 Genotyping for Proper Use of Irinotecan].

    PubMed

    Matsuoka, Ayumu; Ando, Yuichi

    2015-07-01

    Irinotecan is a camptothecin analog used worldwide for a broad range of solid tumors, including colorectal and lung cancers. It can cause severe adverse drug reactions, such as neutropenia or diarrhea. Irinotecan is metabolized to form active SN-38, which is further conjugated and detoxified by the UDP-glucuronosyltransferase (UGT) 1A1 enzyme. Recent pharmacogenetic studies on irinotecan have revealed the impact of UGT1A1 polymorphisms on severe adverse effects. A variant in the promoter of the UGT1A1 gene, the UGT1A1 *28 allele, has been extensively studied, and pharmacogenetic relationships between the variant and severe toxicities of irinotecan have been reported. The US FDA and pharmaceutical companies revised the irinotecan label in 2005, and it now includes homozygosity for the UGT1A1*28 genotype as one of the risk factors for severe neutropenia. A variant in exon 1 of the UGT1A1 gene, the UGT1A1*6 allele, mainly found in East Asians, is also an important risk factor associated with severe neutropenia. The concurrence of UGT1A1*28 and UGT1A1*6, even when heterozygous, markedly alters the disposition of irinotecan, potentially increasing toxicity, which is now written on the label of irinotecan in Japan. For patients showing homozygosity for UGT1A1*28, *6, or compound heterozygosity for UGT1A1*6 and *28, dose reduction of irinotecan is strongly recommended. Genotyping tests for UGT1A1 *6 and *28 have been approved in Japan and are currently used in oncology practice. Moreover, a recent Phase 2 trial for FOLFIRINOX in Japan excluded patients who showed homozygosity for UGT1A1*28, *6, or compound heterozygosity for UGT1A1*6 and *28. At present, irinotecan chemotherapy based on a patient's UGT1A1 genetic status is scientifically reasonable. PMID:26591441

  1. Modernizing medical photography, part 1.

    PubMed

    Crompton, Paul

    2004-12-01

    Government, media and public focus on waiting times in the National Health Service in the United Kingdom has forced the organization to look closely at the process by which a patient progresses through an increasingly complex and ever changing system. In an effort to streamline the patient journey or care pathway, modernizers have turned to business and manufacturing for solutions. Whilst medical photographers need to recognize their role in this context, they are also facing major technological modernization through the development of digital photography. Part 1 of this paper looks at the origins of some of the techniques presently being used to modernize the patient journey. Part 2 shows how these tools of modernization can be utilized to harness the advantages of digital technology to provide a modern and appropriate medical photography service in a large, disparate teaching hospital.

  2. Modernizing medical photography, part 2.

    PubMed

    Crompton, Paul

    2005-03-01

    Part 1 of this paper explored the origins of process activity mapping, one of the major tools currently being used to modernize patient pathways in the National Health Service in Great Britain. Within medical photography the current notion of modernization is inextricably linked to the development of digital technology. Whilst the core principle of capturing light on a sensitive medium remains as clear and relevant as ever, the mechanisms by which the image is processed and presented to the client have changed profoundly. Part 2 shows how the principles of lean thinking and process activity mapping can be utilized to harness the advantages of digital technology to provide a modern and appropriate medical photography service in a large disparate teaching hospital.

  3. The Inconvenient Truth. Part 2

    SciTech Connect

    Athanasiou, T.

    2007-01-15

    Essay-type of publication on what should happen next after Al Gore's presentations on the Inconvenient Truth about the impacts of climate change. The essay states in the first lines: 'We've seen the movie, so we know the first part - we're in trouble deep. And it's time, past time, for at least some of us to go beyond warning to planning, to start talking seriously about a global crash program to stabilize the climate.

  4. The digestive system: part 2.

    PubMed

    Hendry, Charles; Farley, Alistair; McLafferty, Ella; Johnstone, Carolyn

    This article, which forms part of the life sciences series and is the second of two articles on the digestive system, explores the structure and function of the liver, gall bladder and pancreas. It is important that nurses understand how the digestive system works and identify its role in maintaining health. The gross structures of the liver, gall bladder and pancreas associated with the gastrointestinal tract are described, along with relevant physiology and pathologies.

  5. Accurate identification of UDP-glucuronosyltransferase 1A1 (UGT1A1) inhibitors using UGT1A1-overexpressing HeLa cells.

    PubMed

    Sun, Hua; Zhou, Xiaotong; Wu, Baojian

    2015-01-01

    1. UDP-glucuronosyltransferase 1A1 (UGT1A1) plays an irreplaceable role in detoxification of bilirubin and many drugs (e.g., SN-38). Here we aimed to explore the potential of UGT1A1-overexpressing HeLa cells (or HeLa1A1 cells) as a tool to accurately identify UGT1A1 inhibitors. 2. Determination of glucuronidation rates (β-estradiol and SN-38 as the substrates) was performed using HeLa1A1 cells and uridine diphosphoglucuronic acid (UDPGA)-supplemented cDNA expressed UGT1A1 enzyme (or microsomes). The inhibitory effects (IC50 values) of 20 structurally diverse compounds on the UGT1A1 activity were determined using HeLa1A1 cells and microsomal incubations. 3. In HeLa1A1 cells, the IC50 values for inhibition of β-estradiol glucuronidation by the tested compounds ranged from 0.33 to 94.6 µM. In the microsomal incubations, the IC50 values ranged from 0.47 to 155 µM. It was found that the IC50 values of all test compounds derived from the cells were well consistent with those from the microsomes (deviated by less than two-fold). Further, the IC50 values from the cells were strongly correlated with those from microsomes (r = 0.944, p < 0.001). Likewise, the IC50 values (0.37-77.3 µM) for inhibition of SN-38 glucuronidation in the cells were close to those (0.42-122 µM) for glucuronidation inhibition in microsomes. A strong correlation was also observed between the two sets of IC50 values (r = 0.978, p < 0.001). 4. In conclusion, UGT1A1-overexpressing HeLa cells were an appropriate tool to accurately depict the inhibition profiles of chemicals against UGT1A1. PMID:26068529

  6. Mapping of the gene for the Mel{sub 1a}-melatonin receptor to human chromosome 4 (MTNR1A) and mouse chromosome 8 (Mtnr1a)

    SciTech Connect

    Slaugenhaupt, S.A. |; Liebert, C.B.; Altherr, M.R.

    1995-05-20

    The pineal hormone melatonin elicits potent circadian and reproductive effects in mammals. The authors report the chromosomal location of the gene for the Mel{sub 1a}-melatonin receptor that likely mediates these circadian and reproductive actions. PCR analysis of human-rodent somatic cell hybrids showed that the receptor gene (MTNR1A) maps to human chromosome 4q35.1. An interspecific backcross analysis revealed that the mouse gene (Mtnr1a) maps to the proximal portion of chromosome 8. These loci may be involved in genetically based circadian and neuroendocrine disorders. 14 refs., 1 fig.

  7. Strong synergistic induction of CYP1A1 expression by andrographolide plus typical CYP1A inducers in mouse hepatocytes

    SciTech Connect

    Jaruchotikamol, Atika; Jarukamjorn, Kanokwan Sirisangtrakul, Wanna; Sakuma, Tsutomu; Kawasaki, Yuki; Nemoto, Nobuo

    2007-10-15

    The effects of andrographolide, the major diterpenoid constituent of Andrographis paniculata, on the expression of cytochrome P450 superfamily 1 members, including CYP1A1, CYP1A2, and CYP1B1, as well as on aryl hydrocarbon receptor (AhR) expression in primary cultures of mouse hepatocytes were investigated in comparison with the effects of typical CYP1A inducers, including benz[a]anthracene, {beta}-naphthoflavone, and 2,3,7,8-tetrachlorodibenzo-p-dioxin. Andrographolide significantly induced the expression of CYP1A1 and CYP1A2 mRNAs in a concentration-dependent manner, as did the typical CYP1A inducers, but did not induce that of CYP1B1 or AhR. Interestingly, andrographolide plus the typical CYP1A inducers synergistically induced CYP1A1 expression, and the synergism was blocked by an AhR antagonist, resveratrol. The CYP1A1 enzyme activity showed a similar pattern of induction. This is the first report that shows that andrographolide has a potency to induce CYP1A1 enzyme and indicates that andrographolide could be a very useful compound for investigating the regulatory mechanism of the CYP1A1 induction pathway. In addition, our findings suggest preparing advice for rational administration of A. paniculata, according to its ability to induce CYP1A1 expression.

  8. The Growth and Tumor Suppressors NORE1A and RASSF1A Are Targets for Calpain-Mediated Proteolysis

    PubMed Central

    Kuznetsov, Sergey; Khokhlatchev, Andrei V.

    2008-01-01

    Background NORE1A and RASSF1A are growth and tumour suppressors inactivated in a variety of cancers. Methylation of NORE1A and RASSF1A promoters is the predominant mechanism for downregulation of these proteins; however, other mechanisms are likely to exist. Methodology/Principal Findings Here we describe a proteolysis of NORE1A and RASSF1A by calpains as alternative mechanism of their downregulation. Extracts of H358 cell line, a human bronchoalveolar carcinoma, and H460, a large cell carcinoma, were capable of proteolysis of NORE1A protein in the calpain-dependent manner. Likewise, RASSF1A tumor suppressor was proteolyzed by the H358 cell extract. Addition of calpain inhibitor to H358 and H460 cells growing in tissue culture resulted in re-expression of endogenous NORE1A. A survey of 10 human lung tumours revealed that three of them contain an activity capable of inducing NORE1A degradation. Conclusions/Significance Thus, degradation by calpains is a novel mechanism for downregulation of NORE1A and RASSF1A proteins and might be the mechanism allowing cancer cells to escape growth suppression. PMID:19098985

  9. 14 CFR 27.602 - Critical parts.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Critical parts. 27.602 Section 27.602 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: NORMAL CATEGORY ROTORCRAFT Design and Construction General § 27.602 Critical parts. (a) Critical part. A critical part is a part,...

  10. 14 CFR 29.602 - Critical parts.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Critical parts. 29.602 Section 29.602 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Design and Construction General § 29.602 Critical parts. (a) Critical part. A critical part is a part,...

  11. Automated Cutting And Drilling Of Composite Parts

    NASA Technical Reports Server (NTRS)

    Warren, Charles W.

    1993-01-01

    Proposed automated system precisely cuts and drills large, odd-shaped parts made of composite materials. System conceived for manufacturing lightweight composite parts to replace heavier parts in Space Shuttle. Also useful in making large composite parts for other applications. Includes robot locating part to be machined, positions cutter, and positions drill. Gantry-type robot best suited for task.

  12. Effects of Hypoxia Exposure on Hepatic Cytochrome P450 1A (CYP1A) Expression in Atlantic Croaker: Molecular Mechanisms of CYP1A Down-Regulation

    PubMed Central

    Rahman, Md. Saydur; Thomas, Peter

    2012-01-01

    Hypoxia-inducible factor-α (HIF-α) and cytochrome P450 1A (CYP1A) are biomarkers of environmental exposure to hypoxia and organic xenobiotic chemicals that act through the aryl hydrocarbon receptor, respectively. Many aquatic environments heavily contaminated with organic chemicals, such as harbors, are also hypoxic. Recently, we and other scientists reported HIF-α genes are upregulated by hypoxia exposure in aquatic organisms, but the molecular mechanisms of hypoxia regulation of CYP1A expression have not been investigated in teleost fishes. As a first step in understanding the molecular mechanisms of hypoxia modulation of CYP1A expression in fish, we characterized CYP1A cDNA from croaker liver. Hypoxia exposure (dissolved oxygen, DO: 1.7 mg/L for 2 to 4 weeks) caused significant decreases in hepatic CYP1A mRNA and protein levels compared to CYP1A levels in fish held in normoxic conditions. In vivo studies showed that the nitric oxide (NO)-donor, S-nitroso-N-acetyl-DL-penicillamine, significantly decreased CYP1A expression in croaker livers, whereas the competitive inhibitor of NO synthase (NOS), Nω-nitro-L-arginine methyl ester, restored CYP1A mRNA and protein levels in hypoxia-exposed (1.7 mg DO/L for 4 weeks) fish. In vivo hypoxia exposure also markedly increased interleukin-1β (IL-1β, a cytokine), HIF-2α mRNA and endothelial NOS (eNOS) protein levels in croaker livers. Pharmacological treatment with vitamin E, an antioxidant, lowered the IL-1β, HIF-2α mRNA and eNOS protein levels in hypoxia-exposed fish and completely reversed the down-regulation of hepatic CYP1A mRNA and protein levels in response to hypoxia exposure. These results suggest that hypoxia-induced down-regulation of CYP1A is due to alterations of NO and oxidant status, and cellular IL-1β and HIF-α levels. Moreover, the present study provides the first evidence of a role for antioxidants in hepatic eNOS and IL-1β regulation in aquatic vertebrates during hypoxic stress. PMID:22815834

  13. Effects of β-Naphthoflavone on Ugt1a6 and Ugt1a7 Expression in Rat Brain.

    PubMed

    Sakakibara, Yukiko; Katoh, Miki; Kondo, Yuya; Nadai, Masayuki

    2016-01-01

    Uridine 5'-diphosphate-glucuronosyltransferase (UGT) catalyzes a major phase II reaction in a drug-metabolizing enzyme system. Although the UGT1A subfamily is expressed mainly in the liver, it is also expressed in the brain. The purpose of the present study was to elucidate the effect of β-naphthoflavone (BNF), one of the major inducers of drug-metabolizing enzymes, on Ugt1a6 and Ugt1a7 mRNA expression and their glucuronidation in the rat brain. Eight-week-old male Sprague-Dawley rats were treated intraperitoneally with BNF (80 mg/kg), once daily for 7 d. Ugt1a6 and Ugt1a7 mRNA expression increased in the cerebellum and hippocampus (Ugt1a6: 2.1- and 2.3-fold, respectively; Ugt1a7: 1.7- and 2.8-fold, respectively); acetaminophen glucuronidation also increased in the same regions by 4.1- and 2.7-fold, respectively. BNF induced Ugt1a6 and Ugt1a7 mRNA expression and their glucuronidation, and the degree of induction differed among 9 regions. BNF also upregulated CYP1A1, CYP1A2, and CYP1B1 mRNAs in the rat brain. Since the aryl hydrocarbon receptor signaling pathway was activated by BNF, it is indicated that Ugt1a6 and Ugt1a7 were induced via AhR in the rat brain. This study clarified that Ugt1a6 and Ugt1a7 mRNA expression and their enzyme activities were altered by BNF, suggesting that these changes may lead to alteration in the pharmacokinetics of UGT substrate in rat brain. PMID:26725430

  14. Effects of β-Naphthoflavone on Ugt1a6 and Ugt1a7 Expression in Rat Brain.

    PubMed

    Sakakibara, Yukiko; Katoh, Miki; Kondo, Yuya; Nadai, Masayuki

    2016-01-01

    Uridine 5'-diphosphate-glucuronosyltransferase (UGT) catalyzes a major phase II reaction in a drug-metabolizing enzyme system. Although the UGT1A subfamily is expressed mainly in the liver, it is also expressed in the brain. The purpose of the present study was to elucidate the effect of β-naphthoflavone (BNF), one of the major inducers of drug-metabolizing enzymes, on Ugt1a6 and Ugt1a7 mRNA expression and their glucuronidation in the rat brain. Eight-week-old male Sprague-Dawley rats were treated intraperitoneally with BNF (80 mg/kg), once daily for 7 d. Ugt1a6 and Ugt1a7 mRNA expression increased in the cerebellum and hippocampus (Ugt1a6: 2.1- and 2.3-fold, respectively; Ugt1a7: 1.7- and 2.8-fold, respectively); acetaminophen glucuronidation also increased in the same regions by 4.1- and 2.7-fold, respectively. BNF induced Ugt1a6 and Ugt1a7 mRNA expression and their glucuronidation, and the degree of induction differed among 9 regions. BNF also upregulated CYP1A1, CYP1A2, and CYP1B1 mRNAs in the rat brain. Since the aryl hydrocarbon receptor signaling pathway was activated by BNF, it is indicated that Ugt1a6 and Ugt1a7 were induced via AhR in the rat brain. This study clarified that Ugt1a6 and Ugt1a7 mRNA expression and their enzyme activities were altered by BNF, suggesting that these changes may lead to alteration in the pharmacokinetics of UGT substrate in rat brain.

  15. Identification and phylogenetic analysis of novel cytochrome P450 1A genes from ungulate species.

    PubMed

    Darwish, Wageh Sobhy; Kawai, Yusuke; Ikenaka, Yoshinori; Yamamoto, Hideaki; Muroya, Tarou; Ishizuka, Mayumi

    2010-09-01

    As part of an ongoing effort to understand the biological response of wild and domestic ungulates to different environmental pollutants such as dioxin-like compounds, cDNAs encoding for CYP1A1 and CYP1A2 were cloned and characterized. Four novel CYP1A cDNA fragments from the livers of four wild ungulates (elephant, hippopotamus, tapir and deer) were identified. Three fragments from hippopotamus, tapir and deer were classified as CYP1A2, and the other fragment from elephant was designated as CYP1A1/2. The deduced amino acid sequences of these fragment CYP1As showed identities ranging from 76 to 97% with other animal CYP1As. The phylogenetic analysis of these fragments showed that both elephant and hippopotamus CYP1As made separate branches, while tapir and deer CYP1As were located beside that of horse and cattle respectively in the phylogenetic tree. Analysis of dN/dS ratio among the identified CYP1As indicated that odd toed ungulate CYP1A2s were exposed to different selection pressure.

  16. Identification and phylogenetic analysis of novel cytochrome P450 1A genes from ungulate species.

    PubMed

    Darwish, Wageh Sobhy; Kawai, Yusuke; Ikenaka, Yoshinori; Yamamoto, Hideaki; Muroya, Tarou; Ishizuka, Mayumi

    2010-09-01

    As part of an ongoing effort to understand the biological response of wild and domestic ungulates to different environmental pollutants such as dioxin-like compounds, cDNAs encoding for CYP1A1 and CYP1A2 were cloned and characterized. Four novel CYP1A cDNA fragments from the livers of four wild ungulates (elephant, hippopotamus, tapir and deer) were identified. Three fragments from hippopotamus, tapir and deer were classified as CYP1A2, and the other fragment from elephant was designated as CYP1A1/2. The deduced amino acid sequences of these fragment CYP1As showed identities ranging from 76 to 97% with other animal CYP1As. The phylogenetic analysis of these fragments showed that both elephant and hippopotamus CYP1As made separate branches, while tapir and deer CYP1As were located beside that of horse and cattle respectively in the phylogenetic tree. Analysis of dN/dS ratio among the identified CYP1As indicated that odd toed ungulate CYP1A2s were exposed to different selection pressure. PMID:20448415

  17. Selective inhibition of the kinase DYRK1A by targeting its folding process.

    PubMed

    Kii, Isao; Sumida, Yuto; Goto, Toshiyasu; Sonamoto, Rie; Okuno, Yukiko; Yoshida, Suguru; Kato-Sumida, Tomoe; Koike, Yuka; Abe, Minako; Nonaka, Yosuke; Ikura, Teikichi; Ito, Nobutoshi; Shibuya, Hiroshi; Hosoya, Takamitsu; Hagiwara, Masatoshi

    2016-01-01

    Autophosphorylation of amino-acid residues is part of the folding process of various protein kinases. Conventional chemical screening of mature kinases has missed inhibitors that selectively interfere with the folding process. Here we report a cell-based assay that evaluates inhibition of a kinase at a transitional state during the folding process and identify a folding intermediate-selective inhibitor of dual-specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A), which we refer to as FINDY. FINDY suppresses intramolecular autophosphorylation of Ser97 in DYRK1A in cultured cells, leading to its degradation, but does not inhibit substrate phosphorylation catalysed by the mature kinase. FINDY also suppresses Ser97 autophosphorylation of recombinant DYRK1A, suggesting direct inhibition, and shows high selectivity for DYRK1A over other DYRK family members. In addition, FINDY rescues DYRK1A-induced developmental malformations in Xenopus laevis embryos. Our study demonstrates that transitional folding intermediates of protein kinases can be targeted by small molecules, and paves the way for developing novel types of kinase inhibitors. PMID:27102360

  18. Chloroquine impairs visual transduction via modulation of acid sensing ion channel 1a.

    PubMed

    Li, Xiaoyu; Fei, Jianchun; Lei, Zhen; Liu, Kejing; Wu, Jianbo; Meng, Tao; Yu, Jingui; Li, Jingxin

    2014-08-01

    Acid-sensing ion channels (ASICs) are extracellular pH sensors activated by protons, which influence retinal activity and phototransduction. Among all ASICs, ASIC1a is abundantly expressed in the retina and involved in normal retinal activity. Chloroquine, which has been used in the treatment of malaria, rheumatoid arthritis and systemic lupus erythematosus, has been shown to be toxic to the retina. However, the underlying mechanisms remain unclear. In this study, we investigated the role of chloroquine in phototransduction by measuring the electroretinogram (ERG). The effect of chloroquine on acid-evoked currents in either isolated rat retinal ganglion neurons (RGNs) or Chinese hamster ovary (CHO) cells transfected with ASIC1a were assessed using a whole-cell patch-clamp technique. Chloroquine reduced the b-wave of scotopic 0.01 and photopic 3.0 and amplitudes of oscillatory potentials (OPs), an effect which was almost completely reversed by PcTx1, an ASIC1a-specific channel blocker. Further, patch-clamp experiments demonstrated that chloroquine reduced the peak current amplitude and prolonged the activation and desensitization of ASIC1a currents. These chloroquine-induced effects on the kinetics of ASIC 1a were dose-, pH- and Ca(2+)-dependent. Taken together, these results demonstrate that chloroquine affects vision conduction by directly modifying the kinetics of ASIC1a. Such a mechanism, may, in part, explain the retinal toxicity of chloroquine.

  19. Selective inhibition of the kinase DYRK1A by targeting its folding process

    PubMed Central

    Kii, Isao; Sumida, Yuto; Goto, Toshiyasu; Sonamoto, Rie; Okuno, Yukiko; Yoshida, Suguru; Kato-Sumida, Tomoe; Koike, Yuka; Abe, Minako; Nonaka, Yosuke; Ikura, Teikichi; Ito, Nobutoshi; Shibuya, Hiroshi; Hosoya, Takamitsu; Hagiwara, Masatoshi

    2016-01-01

    Autophosphorylation of amino-acid residues is part of the folding process of various protein kinases. Conventional chemical screening of mature kinases has missed inhibitors that selectively interfere with the folding process. Here we report a cell-based assay that evaluates inhibition of a kinase at a transitional state during the folding process and identify a folding intermediate-selective inhibitor of dual-specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A), which we refer to as FINDY. FINDY suppresses intramolecular autophosphorylation of Ser97 in DYRK1A in cultured cells, leading to its degradation, but does not inhibit substrate phosphorylation catalysed by the mature kinase. FINDY also suppresses Ser97 autophosphorylation of recombinant DYRK1A, suggesting direct inhibition, and shows high selectivity for DYRK1A over other DYRK family members. In addition, FINDY rescues DYRK1A-induced developmental malformations in Xenopus laevis embryos. Our study demonstrates that transitional folding intermediates of protein kinases can be targeted by small molecules, and paves the way for developing novel types of kinase inhibitors. PMID:27102360

  20. X-pinch. Part I

    SciTech Connect

    Pikuz, S. A. Shelkovenko, T. A.; Hammer, D. A.

    2015-04-15

    Results of experimental studies of the X-pinch since its invention and implementation in 1982 at the Lebedev Physical Institute are presented. The concept of the X-pinch arose in the course of innovative experiments on studying the generation of hot dense plasma in nanosecond Z-pinches. The main idea was to localize the region occupied by the plasma and stabilize the moment of its formation. This was achieved by using a load in the form of crossed wires. The resulting plasma object turned out to be so interesting that a new scientific direction appeared in plasma physics. At present, studies in this direction are being performed in many laboratories worldwide. The term X-pinch became conventional, and sections devoted to its study are always present at plasma conferences. X-pinch is not only a very interesting scientific object, but also a unique source of X-ray emission with extreme parameters. The use of X-pinches for point-projection X-ray radiography of high-energy-density objects provided many new experimental results. In some cases, the concepts of the processes occurring in matter upon fast energy deposition changed dramatically. The review consists of two parts. The first part briefly outlines the history of creation and studies of X-pinches, describes the diagnostic techniques and devices developed during these studies, and presents the main results obtained in studying the physical processes occurring in the X-pinch. The second part is devoted to the results of detailed studies of the X-pinch hot spot—the region where the highest plasma parameters are achieved and which is a source of X-ray emission with extreme parameters. Some results of X-pinch simulations are also presented.

  1. X-pinch. Part I

    NASA Astrophysics Data System (ADS)

    Pikuz, S. A.; Shelkovenko, T. A.; Hammer, D. A.

    2015-04-01

    Results of experimental studies of the X-pinch since its invention and implementation in 1982 at the Lebedev Physical Institute are presented. The concept of the X-pinch arose in the course of innovative experiments on studying the generation of hot dense plasma in nanosecond Z-pinches. The main idea was to localize the region occupied by the plasma and stabilize the moment of its formation. This was achieved by using a load in the form of crossed wires. The resulting plasma object turned out to be so interesting that a new scientific direction appeared in plasma physics. At present, studies in this direction are being performed in many laboratories worldwide. The term X-pinch became conventional, and sections devoted to its study are always present at plasma conferences. X-pinch is not only a very interesting scientific object, but also a unique source of X-ray emission with extreme parameters. The use of X-pinches for point-projection X-ray radiography of high-energy-density objects provided many new experimental results. In some cases, the concepts of the processes occurring in matter upon fast energy deposition changed dramatically. The review consists of two parts. The first part briefly outlines the history of creation and studies of X-pinches, describes the diagnostic techniques and devices developed during these studies, and presents the main results obtained in studying the physical processes occurring in the X-pinch. The second part is devoted to the results of detailed studies of the X-pinch hot spot—the region where the highest plasma parameters are achieved and which is a source of X-ray emission with extreme parameters. Some results of X-pinch simulations are also presented.

  2. Occlusal disease revisited: Part II.

    PubMed

    Lytle, J D

    2001-06-01

    In part I of this article, the evolution of bruxism from childhood was discussed. Further, the different types of anterior tooth wear were reviewed. Specifically, the type of wear noted in bruxed-braced or cross-over position was pointed out. Examples were illustrated to allow the practitioner to recognize the type of parafunction in advance of treatment. This article will continue the discussion of cross over with moderate to extreme examples. Suggestions for treatment are discussed depending on the severity of the problem. Restorative failure and the implications for implant dentistry are noted. PMID:11490404

  3. Genetic parts to program bacteria.

    PubMed

    Voigt, Christopher A

    2006-10-01

    Genetic engineering is entering a new era, where microorganisms can be programmed using synthetic constructs of DNA encoding logic and operational commands. A toolbox of modular genetic parts is being developed, comprised of cell-based environmental sensors and genetic circuits. Systems have already been designed to be interconnected with each other and interfaced with the control of cellular processes. Engineering theory will provide a predictive framework to design operational multicomponent systems. On the basis of these developments, increasingly complex cellular machines are being constructed to build specialty chemicals, weave biomaterials, and to deliver therapeutics.

  4. How parts make up wholes

    PubMed Central

    Findlay, Scott D.; Thagard, Paul

    2012-01-01

    We propose a schema that characterizes how parts constitute wholes at diverse levels of organization, ranging from the atomic to the biological to the social. This schema of tags, organizers, attachers, and communicators provides a unified understanding of the structure, function, and dynamics of organization in physics, biology, and the cognitive and social sciences. We use this schema to identify and describe structures and processes at many levels of organization, and discuss its relevance for understanding the nature of constitution and emergence, especially the relation between individual humans and the social groups they constitute. PMID:23227011

  5. Understanding Radiation Thermometry. Part I

    NASA Technical Reports Server (NTRS)

    Risch Timothy K.

    2015-01-01

    This document is a two-part course on the theory and practice of radiation thermometry. Radiation thermometry is the technique for determining the temperature of a surface or a volume by measuring the electromagnetic radiation it emits. This course covers the theory and practice of radiative thermometry and emphasizes the modern application of the field using commercially available electronic detectors and optical components. The course covers the historical development of the field, the fundamental physics of radiative surfaces, along with modern measurement methods and equipment.

  6. The Sum of its Parts

    NASA Astrophysics Data System (ADS)

    Spriggs, John

    A safety case is a collation of the arguments and supporting evidence needed to demonstrate that an item is safe to use. Although there are regulatory and legislative requirements in some industries, and in some parts of the world, for the production of a safety case, there are few standard definitions of what should go into one. Indeed, there is no single, correct, way to prepare a safety case; but this should not be surprising, given the wide variety of applications to which they are put.

  7. Understanding Radiation Thermometry. Part II

    NASA Technical Reports Server (NTRS)

    Risch, Timothy K.

    2015-01-01

    This document is a two-part course on the theory and practice of radiation thermometry. Radiation thermometry is the technique for determining the temperature of a surface or a volume by measuring the electromagnetic radiation it emits. This course covers the theory and practice of radiative thermometry and emphasizes the modern application of the field using commercially available electronic detectors and optical components. The course covers the historical development of the field, the fundamental physics of radiative surfaces, along with modern measurement methods and equipment.

  8. The digestive system: part 1.

    PubMed

    Johnstone, Carolyn; Hendry, Charles; Farley, Alistair; McLafferty, Ella

    This article, which forms part of the life sciences series and is the first of two articles on the digestive system, explores the structure and function of the digestive system. It is important that nurses understand how the digestive system works and its role in maintaining health. The article describes the gross structure of the gastrointestinal tract along with relevant physiology. It also outlines several disorders of the gastrointestinal tract and their treatment and nursing management. The second article will explain the liver, pancreas and gall bladder and their digestive functions, and provides a brief overview of the disorders of chronic liver disease, pancreatitis and gallstones.

  9. Organic anion-transporting polypeptide 1a4 (Oatp1a4) is important for secondary bile acid metabolism.

    PubMed

    Zhang, Youcai; Csanaky, Iván L; Selwyn, Felcy Pavithra; Lehman-McKeeman, Lois D; Klaassen, Curtis D

    2013-08-01

    Organic anion transporting polypeptides (human: OATPs; rodent: Oatps) were thought to have important functions in bile acid (BA) transport. Oatp1a1, 1a4, and 1b2 are the three major Oatp1 family members in rodent liver. Our previous studies have characterized the BA homeostasis in Oatp1a1-null and Oatp1b2-null mice. The present study investigated the physiological role of Oatp1a4 in BA homeostasis by using Oatp1a4-null mice. Oatp1a4 expression is female-predominant in livers of mice, and thereby it was expected that female Oatp1a4-null mice will have more prominent changes than males. Interestingly, the present study demonstrated that female Oatp1a4-null mice had no significant alterations in BA concentrations in serum or liver, though they had increased mRNA of hepatic BA efflux transporters (Mrp4 and Ostα/β) and ileal BA transporters (Asbt and Ostα/β). In contrast, male Oatp1a4-null mice showed significantly altered BA homeostasis, including increased concentrations of deoxycholic acid (DCA) in serum, liver and intestinal contents. After feeding a DCA-supplemented diet, male but not female Oatp1a4-null mice had higher concentrations of DCA in serum and livers than their WT controls. This suggested that Oatp1a4 is important for intestinal absorption of secondary BAs in male mice. Furthermore, loss of Oatp1a4 function did not decrease BA accumulation in serum or livers of bile-duct-ligated mice, suggesting that Oatp1a4 is not likely a BA uptake transporter. In summary, the present study for the first time demonstrates that Oatp1a4 does not appear to mediate the hepatic uptake of BAs, but plays an important male-predominant role in secondary BA metabolism in mice.

  10. Intracellular distribution of differentially phosphorylated dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A).

    PubMed

    Kaczmarski, Wojciech; Barua, Madhabi; Mazur-Kolecka, Bozena; Frackowiak, Janusz; Dowjat, Wieslaw; Mehta, Pankaj; Bolton, David; Hwang, Yu-Wen; Rabe, Ausma; Albertini, Giorgio; Wegiel, Jerzy

    2014-02-01

    The gene encoding dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) is located within the Down syndrome (DS) critical region of chromosome 21. DYRK1A interacts with a plethora of substrates in the cytosol, cytoskeleton, and nucleus. Its overexpression is a contributing factor to the developmental alterations and age-associated pathology observed in DS. We hypothesized that the intracellular distribution of DYRK1A and cell-compartment-specific functions are associated with DYRK1A posttranslational modifications. Fractionation showed that, in both human and mouse brain, almost 80% of DYRK1A was associated with the cytoskeleton, and the remaining DYRK1A was present in the cytosolic and nuclear fractions. Coimmunoprecipitation revealed that DYRK1A in the brain cytoskeleton fraction forms complexes with filamentous actin, neurofilaments, and tubulin. Two-dimensional gel analysis of the fractions revealed DYRK1A with distinct isoelectric points: 5.5-6.5 in the nucleus, 7.2-8.2 in the cytoskeleton, and 8.7 in the cytosol. Phosphate-affinity gel electrophoresis demonstrated several bands of DYRK1A with different mobility shifts for nuclear, cytoskeletal, and cytosolic DYRK1A, indicating modification by phosphorylation. Mass spectrometry analysis disclosed one phosphorylated site in the cytosolic DYRK1A and multiple phosphorylated residues in the cytoskeletal DYRK1A, including two not previously described. This study supports the hypothesis that intracellular distribution and compartment-specific functions of DYRK1A may depend on its phosphorylation pattern. PMID:24327345

  11. 40 CFR Table 1a to Subpart Ec of... - Emissions Limits for Small, Medium, and Large HMIWI at Affected Facilities as Defined in § 60.50c...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Large HMIWI at Affected Facilities as Defined in § 60.50c(a)(1) and (2) 1A Table 1A to Subpart Ec of... Waste Incinerators for Which Construction is Commenced After June 20, 1996 Pt. 60, Subpt. Ec, Table 1A Table 1A to Subpart Ec of Part 60—Emissions Limits for Small, Medium, and Large HMIWI at...

  12. 40 CFR Table 1a to Subpart Ec of... - Emissions Limits for Small, Medium, and Large HMIWI at Affected Facilities as Defined in § 60.50c...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Large HMIWI at Affected Facilities as Defined in § 60.50c(a)(1) and (2) 1A Table 1A to Subpart Ec of...: Hospital/Medical/Infectious Waste Incinerators Pt. 60, Subpt. Ec, Table 1A Table 1A to Subpart Ec of Part...(a)(1) and (2) Pollutant Units (7 percent oxygen, dry basis) Emissions limits HMIWI size Small...

  13. 40 CFR Table 1a to Subpart Ec of... - Emissions Limits for Small, Medium, and Large HMIWI at Affected Facilities as Defined in § 60.50c...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Large HMIWI at Affected Facilities as Defined in § 60.50c(a)(1) and (2) 1A Table 1A to Subpart Ec of...: Hospital/Medical/Infectious Waste Incinerators Pt. 60, Subpt. Ec, Table 1A Table 1A to Subpart Ec of Part...(a)(1) and (2) Pollutant Units (7 percent oxygen, dry basis) Emissions limits HMIWI size Small...

  14. 26 CFR 1.666(a)-1A - Amount allocated.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 8 2011-04-01 2011-04-01 false Amount allocated. 1.666(a)-1A Section 1.666(a... Beginning Before January 1, 1969 § 1.666(a)-1A Amount allocated. (a) In general. In the case of a trust that....665(e)-1A(a)(1)(ii) as those beginning after December 31, 1968) according to the amount...

  15. NASA EEE Parts 2014 Year in Review

    NASA Technical Reports Server (NTRS)

    Lee, Sara-Anne

    2015-01-01

    The NASA Electronic Parts and Packaging Program continue to support Electrical, Electronic and Electromagnetic Parts for the agency with an eventful year of workshops, innovations, testing and challenges.

  16. Standards in neurosonology. Part II

    PubMed Central

    Tomczyk, Tomasz; Luchowski, Piotr; Kozera, Grzegorz; Kaźmierski, Radosław; Stelmasiak, Zbigniew

    2016-01-01

    The paper presents standards related to ultrasound imaging of the cerebral vasculature and structures. The aim of this paper is to standardize both the performance and description of ultrasound imaging of the extracranial and intracranial cerebral arteries as well as a study of a specific brain structure, i.e. substantia nigra hyperechogenicity. The following aspects are included in the description of standards for each ultrasonographic method: equipment requirements, patient preparation, study technique and documentation as well as the required elements of ultrasound description. Practical criteria for the diagnosis of certain pathologies in accordance with the latest literature were also presented. Furthermore, additional comments were included in some of the sections. Part I discusses standards for the performance, documentation and description of different ultrasound methods (Duplex, Doppler). Part II and III are devoted to standards for specific clinical situations (vasospasm, monitoring after the acute stage of stroke, detection of a right-to-left shunts, confirmation of the arrest of the cerebral circulation, an assessment of the functional efficiency of circle of Willis, an assessment of the cerebrovascular vasomotor reserve as well as the measurement of substantia nigra hyperechogenicity). PMID:27104002

  17. Future Jet Technologies, Part C

    NASA Astrophysics Data System (ADS)

    Gal-Or, Benjamin

    2013-06-01

    This updated, PART C REVIEW, covers the dangerous global spread of JS-stealth, drone technology [1-91], canard-free, stealth, new agile drones and the highly debated, 480 billion F-35 International Program as reported on March 13, 2013 to the U.S. Congress [92]. Due to serious design issues, spiraling high costs and years in delays, alternatives are analyzed here, depicted and proposed, mainly from the propulsion-design point of view. These include fleets of low-cost, stealth, jet-steered-drones mixed with non-stealthy, low-cost, ready-to-be-delivered, U.S. or European or Russian fighter aircraft. Can a few F-35s win against large fleets of stealth agile drones? To understand the dangers and critical issues involved, the author's own, past classified information is partly disclosed, while resorting to images in Figs. 2(a) and 2(b) taken from his book [2] and from Wikipedia, the "Free Encyclopedia", in all other images.

  18. Standards in neurosonology. Part III.

    PubMed

    Wojczal, Joanna; Tomczyk, Tomasz; Luchowski, Piotr; Kozera, Grzegorz; Kaźmierski, Radosław; Stelmasiak, Zbigniew

    2016-06-01

    The paper presents standards related to ultrasound imaging of the cerebral vasculature and structures. The aim of this paper is to standardize both the performance and description of ultrasound imaging of the extracranial and intracranial cerebral arteries as well as a study of a specific brain structure, i.e. substantia nigra hyperechogenicity. The following aspects are included in the description of standards for each ultrasonographic method: equipment requirements, patient preparation, study technique and documentation as well as the required elements of ultrasound description. Practical criteria for the diagnosis of certain pathologies in accordance with the latest literature were also presented. Furthermore, additional comments were included in some of the sections. Part I discusses standards for the performance, documentation and description of different ultrasound methods (Duplex, Doppler). Part II and III are devoted to standards for specific clinical situations (vasospasm, monitoring after the acute stage of stroke, detection of a right-to-left shunts, confirmation of the arrest of the cerebral circulation, an assessment of the functional efficiency of circle of Willis, an assessment of the cerebrovascular vasomotor reserve as well as the measurement of substantia nigra hyperechogenicity). PMID:27446600

  19. Standards in neurosonology. Part III

    PubMed Central

    Tomczyk, Tomasz; Luchowski, Piotr; Kozera, Grzegorz; Kaźmierski, Radosław; Stelmasiak, Zbigniew

    2016-01-01

    The paper presents standards related to ultrasound imaging of the cerebral vasculature and structures. The aim of this paper is to standardize both the performance and description of ultrasound imaging of the extracranial and intracranial cerebral arteries as well as a study of a specific brain structure, i.e. substantia nigra hyperechogenicity. The following aspects are included in the description of standards for each ultrasonographic method: equipment requirements, patient preparation, study technique and documentation as well as the required elements of ultrasound description. Practical criteria for the diagnosis of certain pathologies in accordance with the latest literature were also presented. Furthermore, additional comments were included in some of the sections. Part I discusses standards for the performance, documentation and description of different ultrasound methods (Duplex, Doppler). Part II and III are devoted to standards for specific clinical situations (vasospasm, monitoring after the acute stage of stroke, detection of a right-to-left shunts, confirmation of the arrest of the cerebral circulation, an assessment of the functional efficiency of circle of Willis, an assessment of the cerebrovascular vasomotor reserve as well as the measurement of substantia nigra hyperechogenicity). PMID:27446600

  20. Standards in neurosonology. Part III.

    PubMed

    Wojczal, Joanna; Tomczyk, Tomasz; Luchowski, Piotr; Kozera, Grzegorz; Kaźmierski, Radosław; Stelmasiak, Zbigniew

    2016-06-01

    The paper presents standards related to ultrasound imaging of the cerebral vasculature and structures. The aim of this paper is to standardize both the performance and description of ultrasound imaging of the extracranial and intracranial cerebral arteries as well as a study of a specific brain structure, i.e. substantia nigra hyperechogenicity. The following aspects are included in the description of standards for each ultrasonographic method: equipment requirements, patient preparation, study technique and documentation as well as the required elements of ultrasound description. Practical criteria for the diagnosis of certain pathologies in accordance with the latest literature were also presented. Furthermore, additional comments were included in some of the sections. Part I discusses standards for the performance, documentation and description of different ultrasound methods (Duplex, Doppler). Part II and III are devoted to standards for specific clinical situations (vasospasm, monitoring after the acute stage of stroke, detection of a right-to-left shunts, confirmation of the arrest of the cerebral circulation, an assessment of the functional efficiency of circle of Willis, an assessment of the cerebrovascular vasomotor reserve as well as the measurement of substantia nigra hyperechogenicity).

  1. Standards in neurosonology. Part I.

    PubMed

    Wojczal, Joanna; Tomczyk, Tomasz; Luchowski, Piotr; Kozera, Grzegorz; Kaźmierski, Radosław; Stelmasiak, Zbigniew

    2015-09-01

    The paper presents standards related to ultrasound imaging of the cerebral vasculature and structures. The aim of this paper is to standardize both the performance and description of ultrasound imaging of the extracranial and intracranial cerebral arteries as well as a study of a specific brain structure, i.e. substantia nigra hyperechogenicity. The following aspects are included in the description of standards for each ultrasonographic method: equipment requirements, patient preparation, study technique and documentation as well as the required elements of ultrasound description. Practical criteria for the diagnosis of certain pathologies in accordance with the latest literature were also presented. Furthermore, additional comments were included in some of the sections. Part I discusses standards for the performance, documentation and description of different ultrasound methods (Duplex, Doppler). Part II and III are devoted to standards for specific clinical situations (vasospasm, monitoring after the acute stage of stroke, detection of a right-toleft shunts, confirmation of the arrest of the cerebral circulation, an assessment of the functional efficiency of circle of Willis, an assessment of the cerebrovascular vasomotor reserve as well as the measurement of substantia nigra hyperechogenicity).

  2. Standards in neurosonology. Part II.

    PubMed

    Wojczal, Joanna; Tomczyk, Tomasz; Luchowski, Piotr; Kozera, Grzegorz; Kaźmierski, Radosław; Stelmasiak, Zbigniew

    2016-03-01

    The paper presents standards related to ultrasound imaging of the cerebral vasculature and structures. The aim of this paper is to standardize both the performance and description of ultrasound imaging of the extracranial and intracranial cerebral arteries as well as a study of a specific brain structure, i.e. substantia nigra hyperechogenicity. The following aspects are included in the description of standards for each ultrasonographic method: equipment requirements, patient preparation, study technique and documentation as well as the required elements of ultrasound description. Practical criteria for the diagnosis of certain pathologies in accordance with the latest literature were also presented. Furthermore, additional comments were included in some of the sections. Part I discusses standards for the performance, documentation and description of different ultrasound methods (Duplex, Doppler). Part II and III are devoted to standards for specific clinical situations (vasospasm, monitoring after the acute stage of stroke, detection of a right-to-left shunts, confirmation of the arrest of the cerebral circulation, an assessment of the functional efficiency of circle of Willis, an assessment of the cerebrovascular vasomotor reserve as well as the measurement of substantia nigra hyperechogenicity).

  3. Microbial Induction of Inflammatory Bowel Disease Associated Gene TL1A (TNFSF15) in Antigen Presenting Cells

    PubMed Central

    Shih, David Q.; Kwan, Lola Y.; Chavez, Valerie; Cohavy, Offer; Gonsky, Rivkah; Chang, Elmer Y.; Chang, Christopher; Elson, Charles O.; Targan, Stephan R.

    2010-01-01

    Summary TL1A is a member of the TNF superfamily and its expression is increased in the mucosa of inflammatory bowel disease (IBD) patients. Neutralizing anti-mouse TL1A Ab attenuates chronic colitis in two T cell driven murine models, suggesting that TL1A is a central modulator of gut mucosal inflammation in IBD. We showed previously that TL1A is induced by immune complexes (IC) via the FcγR signaling pathway. In this study, we report that multiple bacteria, including gram negative organisms (E. coli, E. coli Nissle 1917, S. typhimurium), gram positive organisms (L. monocytogenes, S. epidermidis), partial anaerobes (C. jejuni), and obligate anaerobes (B. thetaiotaomicron, B. breve, Clostridium A4) activate TL1A expression in human APC, including monocytes and monocyte-derived DC. Bacterially induced TL1A mRNA expression correlates with the detection of TL1A protein levels. TL1A induced by bacteria is mediated in part by the TLR signaling pathway and inhibited by downstream blockade of p38 MAPK and NF-κB activation. Microbial induction of TL1A production by human APC potentiated CD4+ T cell effector function by augmenting IFN-γ production. Our findings suggest a role for TL1A in pro-inflammatory APC-T cell interactions and implicate TL1A in host responses to enteric microorganisms. PMID:19839006

  4. Microbial induction of inflammatory bowel disease associated gene TL1A (TNFSF15) in antigen presenting cells.

    PubMed

    Shih, David Q; Kwan, Lola Y; Chavez, Valerie; Cohavy, Offer; Gonsky, Rivkah; Chang, Elmer Y; Chang, Christopher; Elson, Charles O; Targan, Stephan R

    2009-11-01

    TL1A is a member of the TNF superfamily and its expression is increased in the mucosa of inflammatory bowel disease patients. Neutralizing anti-mouse TL1A Ab attenuates chronic colitis in two T-cell driven murine models, suggesting that TL1A is a central modulator of gut mucosal inflammation in inflammatory bowel disease. We showed previously that TL1A is induced by immune complexes via the Fc gamma R signaling pathway. In this study, we report that multiple bacteria, including gram negative organisms (E. coli, E. coli Nissle 1917, Salmonella typhimurium), gram positive organisms (Listeria monocytogenes, Staphylococcus epidermidis), partial anaerobes (Campylobacter jejuni), and obligate anaerobes (Bacteroides thetaiotaomicron, Bifidobacterium breve, Clostridium A4) activate TL1A expression in human APC, including monocytes and monocyte-derived DC. Bacterially induced TL1A mRNA expression correlates with the detection of TL1A protein levels. TL1A induced by bacteria is mediated in part by the TLR signaling pathway and inhibited by downstream blockade of p38 MAPK and NF-kappaB activation. Microbial induction of TL1A production by human APC potentiated CD4(+) T-cell effector function by augmenting IFN-gamma production. Our findings suggest a role for TL1A in pro-inflammatory APC-T cell interactions and implicate TL1A in host responses to enteric microorganisms.

  5. Photodissociation of N2O: excitation of 1A" states.

    PubMed

    Schinke, Reinhard; Schmidt, Johan A

    2012-11-26

    We investigate the contributions of the lowest two (1)A" states in the UV photodissociation of N(2)O employing three-dimensional potential energy surfaces and transition dipole moment functions. Because the transition dipole moments are much smaller than for the 2 (1)A' state, we conclude that excitation of the (1)A" states has a marginal effect. The dense vibrational spectrum of the quasi-bound 2(1)A" state possibly explains some of the tiny, noise-like structures of the measured absorption spectrum. PMID:22536943

  6. [Autoimmune diseases in type 1A diabetes mellitus].

    PubMed

    Ferreira-Hermosillo, Aldo; Molina-Ayala, Mario Antonio

    2015-08-01

    Type 1A diabetes (DM1A) is an autoimmune disease that comprises 10% of patients with diabetes mellitus. Its frequency is gradually increasing in countries like Mexico. Patients with DM1A commonly have hypothyroidism, Addison disease, celiac disease and less common diseases such as polyglandular syndrome. These diseases are related to susceptibility genes such as HLA, CTLA-4 and PTPN22, which induce central and peripheral immunologic tolerance. This review article emphasizes the importance of searching other autoimmune diseases in patients with DM1A, to improve their prognosis and quality of life.

  7. Aldehyde dehydrogenase 1A1 in stem cells and cancer

    PubMed Central

    Tomita, Hiroyuki; Tanaka, Kaori; Tanaka, Takuji; Hara, Akira

    2016-01-01

    The human genome contains 19 putatively functional aldehyde dehydrogenase (ALDH) genes, which encode enzymes critical for detoxification of endogenous and exogenous aldehyde substrates through NAD(P)+-dependent oxidation. ALDH1 has three main isotypes, ALDH1A1, ALDH1A2, and ALDH1A3, and is a marker of normal tissue stem cells (SC) and cancer stem cells (CSC), where it is involved in self-renewal, differentiation and self-protection. Experiments with murine and human cells indicate that ALDH1 activity, predominantly attributed to isotype ALDH1A1, is tissue- and cancer-specific. High ALDH1 activity and ALDH1A1 overexpression are associated with poor cancer prognosis, though high ALDH1 and ALDH1A1 levels do not always correlate with highly malignant phenotypes and poor clinical outcome. In cancer therapy, ALDH1A1 provides a useful therapeutic CSC target in tissue types that normally do not express high levels of ALDH1A1, including breast, lung, esophagus, colon and stomach. Here we review the functions and mechanisms of ALDH1A1, the key ALDH isozyme linked to SC populations and an important contributor to CSC function in cancers, and we outline its potential in future anticancer strategies. PMID:26783961

  8. Stiffness of modified Type 1a linear external skeletal fixators.

    PubMed

    Reaugh, H F; Rochat, M C; Bruce, C W; Galloway, D S; Payton, M E

    2007-01-01

    Modifications of a Type 1a external skeletal fixator (ESF) frame were evaluated by alternately placing transfixation pins on opposite sides of the connecting rod (Type 1a-MOD) or by placing additional connecting rods on either of the two inside (Type 1a-INSIDE) or two outside (Type 1a-OUTSIDE) transfixation pins. The objective of this study was to evaluate the stiffness of these modifications in terms of axial compression (AC), cranial-caudal bending (CCB), and medial-lateral bending (MLB). We hypothesized that these designs would allow significant increase in unilateral frame stiffness, over Type 1a, without proportional increase in frame complexity or technical difficulty of application. All of the ESF frames were constructed using large IMEX SKtrade mark clamps, 3.2 mm threaded fixation pins, 9.5 mm carbon fibre connecting rods and Delrin rods as bone models. Nine, eight pin frames of each design were constructed, and subjected to repetitive non-destructive loading forces (AC, CCB, MLB) using a materials testing machine. Frame construct stiffness for each force (AC, CCB, MLB) was derived from load-deformation curve analysis and displayed in N/mm. Data revealed the 1a-MOD and 1a-OUTSIDE constructs had significantly increased stiffness in CCB and AC as compared to the Type 1a constructs while all of the modified constructs were significantly stiffer in MLB than the Type 1a constructs. PMID:18038001

  9. Screening of CACNA1A and ATP1A2 genes in hemiplegic migraine: clinical, genetic, and functional studies

    PubMed Central

    Carreño, Oriel; Corominas, Roser; Serra, Selma Angèlica; Sintas, Cèlia; Fernández-Castillo, Noèlia; Vila-Pueyo, Marta; Toma, Claudio; Gené, Gemma G; Pons, Roser; Llaneza, Miguel; Sobrido, María-Jesús; Grinberg, Daniel; Valverde, Miguel Ángel; Fernández-Fernández, José Manuel; Macaya, Alfons; Cormand, Bru

    2013-01-01

    Hemiplegic migraine (HM) is a rare and severe subtype of autosomal dominant migraine, characterized by a complex aura including some degree of motor weakness. Mutations in four genes (CACNA1A, ATP1A2, SCN1A and PRRT2) have been detected in familial and in sporadic cases. This genetically and clinically heterogeneous disorder is often accompanied by permanent ataxia, epileptic seizures, mental retardation, and chronic progressive cerebellar atrophy. Here we report a mutation screening in the CACNA1A and ATP1A2 genes in 18 patients with HM. Furthermore, intragenic copy number variant (CNV) analysis was performed in CACNA1A using quantitative approaches. We identified four previously described missense CACNA1A mutations (p.Ser218Leu, p.Thr501Met, p.Arg583Gln, and p.Thr666Met) and two missense changes in the ATP1A2 gene, the previously described p.Ala606Thr and the novel variant p.Glu825Lys. No structural variants were found. This genetic screening allowed the identification of more than 30% of the disease alleles, all present in a heterozygous state. Functional consequences of the CACNA1A-p.Thr501Met mutation, previously described only in association with episodic ataxia, and ATP1A2-p.Glu825Lys, were investigated by means of electrophysiological studies, cell viability assays or Western blot analysis. Our data suggest that both these variants are disease-causing. PMID:24498617

  10. Modulation of anxiety by cortical serotonin 1A receptors

    PubMed Central

    Piszczek, Lukasz; Piszczek, Agnieszka; Kuczmanska, Joanna; Audero, Enrica; Gross, Cornelius T.

    2015-01-01

    Serotonin (5-HT) plays an important role in the modulation of behavior across animal species. The serotonin 1A receptor (Htr1a) is an inhibitory G-protein coupled receptor that is expressed both on serotonin and non-serotonin neurons in mammals. Mice lacking Htr1a show increased anxiety behavior suggesting that its activation by serotonin has an anxiolytic effect. This outcome can be mediated by either Htr1a population present on serotonin (auto-receptor) or non-serotonin neurons (hetero-receptor), or both. In addition, both transgenic and pharmacological studies have shown that serotonin acts on Htr1a during development to modulate anxiety in adulthood, demonstrating a function for this receptor in the maturation of anxiety circuits in the brain. However, previous studies have been equivocal about which Htr1a population modulates anxiety behavior, with some studies showing a role of Htr1a hetero-receptor and others implicating the auto-receptor. In particular, cell-type specific rescue and suppression of Htr1a expression in either forebrain principal neurons or brainstem serotonin neurons reached opposite conclusions about the role of the two populations in the anxiety phenotype of the knockout. One interpretation of these apparently contradictory findings is that the modulating role of these two populations depends on each other. Here we use a novel Cre-dependent inducible allele of Htr1a in mice to show that expression of Htr1a in cortical principal neurons is sufficient to modulate anxiety. Together with previous findings, these results support a hetero/auto-receptor interaction model for Htr1a function in anxiety. PMID:25759645

  11. The roles of CC2D1A and HTR1A gene expressions in autism spectrum disorders.

    PubMed

    Sener, Elif Funda; Cıkılı Uytun, Merve; Korkmaz Bayramov, Keziban; Zararsiz, Gokmen; Oztop, Didem Behice; Canatan, Halit; Ozkul, Yusuf

    2016-06-01

    Classical autism belongs to a group of heterogeneous disorders known as autism spectrum disorders (ASD). Autism is defined as a neurodevelopmental disorder, characterized by repetitive stereotypic behaviors or restricted interests, social withdrawal, and communication deficits. Numerous susceptibility genes and chromosomal abnormalities have been reported in association with autism but the etiology of this disorder is unknown in many cases. CC2D1A gene has been linked to mental retardation (MR) in a family with a large deletion before. Intellectual disability (ID) is a common feature of autistic cases. Therefore we aimed to investigate the expressions of CC2D1A and HTR1A genes with the diagnosis of autism in Turkey. Forty-four autistic patients (35 boys, 9 girls) and 27 controls were enrolled and obtained whole blood samples to isolate RNA samples from each participant. CC2D1A and HTR1A gene expressions were assessed by quantitative Real-Time PCR (qRT-PCR) in Genome and Stem Cell Center, Erciyes University. Both expressions of CC2D1A and HTR1A genes studied on ASD cases and controls were significantly different (p < 0.001). The expression of HTR1A was undetectable in the ASD samples. Comparison of ID and CC2D1A gene expression was also found statistically significant (p = 0.028). CC2D1A gene expression may be used as a candidate gene for ASD cases with ID. Further studies are needed to investigate the potential roles of these CC2D1A and HTR1A genes in their related pathways in ASD.

  12. Revenue cycle management, Part II.

    PubMed

    Crew, Matt

    2007-01-01

    The proper management of your revenue cycle requires the application of "best practices" and the continual monitoring and measuring of the entire cycle. The correct technology will enable you to gain the insight and efficiencies needed in the ever-changing healthcare economy. The revenue cycle is a process that begins when you negotiate payor contracts, set fees, and schedule appointments and continues until claims are paid in full. Every single step in the cycle carries equal importance. Monitoring all phases and a commitment to continually communicating the results will allow you to achieve unparalleled success. In part I of this article, we explored the importance of contracting, scheduling, and case management as well as coding and clinical documentation. We will now take a closer look at the benefits charge capture, claim submission, payment posting, accounts receivable follow-up, and reporting can mean to your practice.

  13. Forming parts over small radii

    NASA Astrophysics Data System (ADS)

    Hazra, S. K.; Hughes, D. J.; Pereira, M. P.; Rolfe, B. F.

    2016-08-01

    Stamping simulations usually make the plane stress simplifying assumption. However, this becomes less valid when material draws around features with radius to sheet thickness ratios less than 20. Pereira, Yan & Rolfe (Wear, Vol.265, p.1687 (2008)) predicted that out-of-plane stress equivalent to material yield can occur because a line contact forms briefly at the start of the draw process. The high transient stress can cause high rates of tool wear and may cause the ‘die impact line’ cosmetic defect. In this work, we present residual strain results of a channel section that was drawn over a small radius. Using the neutron source at the Institut Laue-Langevin, in-plane and out-of-plane strains were measured in the channel part to show some support for the conclusions of Pereira et. al.

  14. 26 CFR 1.669(a)-1A - Amount allocated.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 8 2011-04-01 2011-04-01 false Amount allocated. 1.669(a)-1A Section 1.669(a... Beginning Before January 1, 1969 § 1.669(a)-1A Amount allocated. (a) In general. After a trust has... preceding taxable year is the amount of undistributed capital gain for that preceding taxable year....

  15. Paresev 1-A and Tow Plane with Crew and Pilot

    NASA Technical Reports Server (NTRS)

    1962-01-01

    With the Paresev 1-A and the 450-hp Stearman Sport Biplane as a backdrop the pilot and crew pose for this picture in 1962. Starting at left: On the motorcycle is Walter Whiteside, in the Paresev 1-A is test pilot Milton Thompson, Frank Fedor, Richard Klein, Victor Horton, Tom Kelly, Jr., Fred Harris, owner of the Stearman, John Orahood, and Gary Layton.

  16. Microarray analysis of Xenopus endoderm expressing Ptf1a

    PubMed Central

    Bilogan, Cassandra K.; Horb, Marko E.

    2012-01-01

    Pancreas specific transcription factor 1a (Ptf1a), a bHLH transcription factor, has two temporally distinct functions during pancreas development; initially it is required for early specification of the entire pancreas, while later it is required for proper differentiation and maintenance of only acinar cells. The importance of Ptf1a function was revealed by the fact that loss of Ptf1a leads to pancreas agenesis in humans. While Ptf1a is one of the most important pancreatic transcription factors, little is known about the differences between the regulatory networks it controls during initial specification of the pancreas as opposed to acinar cell development, and to date no comprehensive analysis of its downstream targets has been published. In this paper, we use Xenopus embryos to identify putative downstream targets of Ptf1a. We isolated anterior endoderm tissue overexpressing Ptf1a at two early stages, NF32 and NF36, and compared their gene expression profiles using microarrays. Our results revealed that Ptf1a regulates genes with a wide variety of functions, providing insight into the complexity of the regulatory network required for pancreas specification. PMID:22815262

  17. The protein kinase A regulatory subunit R1A (Prkar1a) plays critical roles in peripheral nerve development.

    PubMed

    Guo, Li; Lee, Audrey A; Rizvi, Tilat A; Ratner, Nancy; Kirschner, Lawrence S

    2013-11-13

    Signaling through cAMP has been implicated in Schwann cell (SC) proliferation and myelination, but the signaling pathway components downstream of cAMP required for SC function remain unknown. Protein kinase A (PKA) is a potential downstream effector of cAMP. Here, we induced loss of Prkar1a, the gene encoding the type 1A regulatory subunit of PKA, in SC to study its role in nerve development; loss of Prkar1a is predicted to elevate PKA activity. Conditional Prkar1a knock-out in mouse SC (Prkar1a-SCKO) resulted in a dramatic and persistent axonal sorting defect, and unexpectedly decreased SC proliferation in Prkar1a-SCKO nerves in vivo. Effects were cell autonomous as they were recapitulated in vitro in Prkar1a-SCKO SC, which showed elevated PKA activity. In the few SCs sorted into 1:1 relationships with axons in vivo, SC myelination was premature in Prkar1a-SCKO nerves, correlating with global increase in the cAMP-regulated transcription factor Oct-6 and expression of myelin basic protein. These data reveal a previously unknown role of PKA in axon sorting, an unexpected inhibitory role of PKA on SC cell proliferation in vivo and define the importance of Prkar1a in peripheral nerve development. PMID:24227708

  18. Environmental enrichment rescues DYRK1A activity and hippocampal adult neurogenesis in TgDyrk1A.

    PubMed

    Pons-Espinal, Meritxell; Martinez de Lagran, Maria; Dierssen, Mara

    2013-12-01

    Hippocampal adult neurogenesis disruptions have been suggested as one of the neuronal plasticity mechanisms underlying learning and memory impairment in Down syndrome (DS). However, it remains unknown whether specific candidate genes are implicated in these phenotypes in the multifactorial context of DS. Here we report that transgenic mice (TgDyrk1A) with overdosage of Dyrk1A, a DS candidate gene, show important alterations in adult neurogenesis including reduced cell proliferation rate, altered cell cycle progression and reduced cell cycle exit leading to premature migration, differentiation and reduced survival of newly born cells. In addition, less proportion of newborn hippocampal TgDyrk1A neurons are activated upon learning, suggesting reduced integration in learning circuits. Some of these alterations were DYRK1A kinase-dependent since we could rescue those using a DYRK1A inhibitor, epigallocatechin-3-gallate. Environmental enrichment also normalized DYRK1A kinase overdosage in the hippocampus, and rescued adult neurogenesis alterations in TgDyrk1A mice. We conclude that Dyrk1A is a good candidate to explain neuronal plasticity deficits in DS and that normalizing the excess of DYRK1A kinase activity either pharmacologically or using environmental stimulation can correct adult neurogenesis defects in DS.

  19. Environmental enrichment rescues DYRK1A activity and hippocampal adult neurogenesis in TgDyrk1A.

    PubMed

    Pons-Espinal, Meritxell; Martinez de Lagran, Maria; Dierssen, Mara

    2013-12-01

    Hippocampal adult neurogenesis disruptions have been suggested as one of the neuronal plasticity mechanisms underlying learning and memory impairment in Down syndrome (DS). However, it remains unknown whether specific candidate genes are implicated in these phenotypes in the multifactorial context of DS. Here we report that transgenic mice (TgDyrk1A) with overdosage of Dyrk1A, a DS candidate gene, show important alterations in adult neurogenesis including reduced cell proliferation rate, altered cell cycle progression and reduced cell cycle exit leading to premature migration, differentiation and reduced survival of newly born cells. In addition, less proportion of newborn hippocampal TgDyrk1A neurons are activated upon learning, suggesting reduced integration in learning circuits. Some of these alterations were DYRK1A kinase-dependent since we could rescue those using a DYRK1A inhibitor, epigallocatechin-3-gallate. Environmental enrichment also normalized DYRK1A kinase overdosage in the hippocampus, and rescued adult neurogenesis alterations in TgDyrk1A mice. We conclude that Dyrk1A is a good candidate to explain neuronal plasticity deficits in DS and that normalizing the excess of DYRK1A kinase activity either pharmacologically or using environmental stimulation can correct adult neurogenesis defects in DS. PMID:23969234

  20. Glucuronidation of OTS167 in Humans Is Catalyzed by UDP-Glucuronosyltransferases UGT1A1, UGT1A3, UGT1A8, and UGT1A10

    PubMed Central

    Ramírez, Jacqueline; Mirkov, Snezana; House, Larry K.

    2015-01-01

    OTS167 is a potent maternal embryonic leucine zipper kinase inhibitor undergoing clinical testing as antineoplastic agent. We aimed to identify the UDP-glucuronosyltransferases (UGTs) involved in OTS167 metabolism, study the relationship between UGT genetic polymorphisms and hepatic OTS167 glucuronidation, and investigate the inhibitory potential of OTS167 on UGTs. Formation of a single OTS167-glucuronide (OTS167-G) was observed in pooled human liver (HLM) (Km = 3.4 ± 0.2 µM), intestinal microsomes (HIM) (Km = 1.7 ± 0.1 µM), and UGTs. UGT1A1 (64 µl/min/mg) and UGT1A8 (72 µl/min/mg) exhibited the highest intrinsic clearances (CLint) for OTS167, followed by UGT1A3 (51 µl/min/mg) and UGT1A10 (47 µl/min/mg); UGT1A9 was a minor contributor. OTS167 glucuronidation in HLM was highly correlated with thyroxine glucuronidation (r = 0.91, P < 0.0001), SN-38 glucuronidation (r = 0.79, P < 0.0001), and UGT1A1 mRNA (r = 0.72, P < 0.0001). Nilotinib (UGT1A1 inhibitor) and emodin (UGT1A8 and UGT1A10 inhibitor) exhibited the highest inhibitory effects on OTS167-G formation in HLM (68%) and HIM (47%). We hypothesize that OTS167-G is an N-glucuronide according to mass spectrometry. A significant association was found between rs6706232 and reduced OTS167-G formation (P = 0.03). No or weak UGT inhibition (range: 0–21%) was observed using clinically relevant OTS167 concentrations (0.4–2 µM). We conclude that UGT1A1 and UGT1A3 are the main UGTs responsible for hepatic formation of OTS167-G. Intestinal UGT1A1, UGT1A8, and UGT1A10 may contribute to first-pass OTS167 metabolism after oral administration. PMID:25870101

  1. Molecular dynamics studies of U1A-RNA complexes.

    PubMed Central

    Reyes, C M; Kollman, P A

    1999-01-01

    The U1A protein binds to a hairpin RNA and an internal-loop RNA with picomolar affinities. To probe the molecular basis of U1A binding, we performed state-of-the-art nanosecond molecular dynamics simulations on both complexes. The good agreement with experimental structures supports the protocols used in the simulations. We compare the dynamics, hydrogen-bonding occupancies, and interfacial flexibility of both complexes and also describe a rigid-body motion in the U1A-internal loop complex that is not observed in the U1A-hairpin simulation. We relate these observations to experimental mutational studies and highlight their significance in U1A binding affinity and specificity. PMID:10024175

  2. Holding Irregularly Shaped Parts For Machining

    NASA Technical Reports Server (NTRS)

    Hilton, Burt W.; Wilson, Rick R.

    1988-01-01

    Part with complicated, irregular outside contours clamped for precise machining of internal passages with aid of simple method. Exterior of part cast in Rigidex, or equivalent epoxy. Forms wall to be clamped. Interior of part machined to finish of 125 microinches or finer. When machining finished, epoxy melted away by heating part to 200 degree F.

  3. 30 CFR 33.38 - Electrical parts.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Electrical parts. 33.38 Section 33.38 Mineral... § 33.38 Electrical parts. (a) Units with electrical parts and designed to operate as electric face... which is Schedule 2F), and the examination and testing of the electrical parts shall be...

  4. High variability and non-neutral evolution of the mammalian avpr1a gene

    PubMed Central

    Fink, Sabine; Excoffier, Laurent; Heckel, Gerald

    2007-01-01

    Background The arginine-vasopressin 1a receptor has been identified as a key determinant for social behaviour in Microtus voles, humans and other mammals. Nevertheless, the genetic bases of complex phenotypic traits like differences in social and mating behaviour among species and individuals remain largely unknown. Contrary to previous studies focusing on differences in the promotor region of the gene, we investigate here the level of functional variation in the coding region (exon 1) of this locus. Results We detected high sequence diversity between higher mammalian taxa as well as between species of the genus Microtus. This includes length variation and radical amino acid changes, as well as the presence of distinct protein variants within individuals. Additionally, negative selection prevails on most parts of the first exon of the arginine-vasopressin receptor 1a (avpr1a) gene but it contains regions with higher rates of change that harbour positively selected sites. Synonymous and non-synonymous substitution rates in the avpr1a gene are not exceptional compared to other genes, but they exceed those found in related hormone receptors with similar functions. Discussion These results stress the importance of considering variation in the coding sequence of avpr1a in regards to associations with life history traits (e.g. social behaviour, mating system, habitat requirements) of voles, other mammals and humans in particular. PMID:17900345

  5. Method of forming and assembly of parts

    DOEpatents

    Ripley, Edward B.

    2010-12-28

    A method of assembling two or more parts together that may be metal, ceramic, metal and ceramic parts, or parts that have different CTE. Individual parts are formed and sintered from particles that leave a network of interconnecting porosity in each sintered part. The separate parts are assembled together and then a fill material is infiltrated into the assembled, sintered parts using a method such as capillary action, gravity, and/or pressure. The assembly is then cured to yield a bonded and fully or near-fully dense part that has the desired physical and mechanical properties for the part's intended purpose. Structural strength may be added to the parts by the inclusion of fibrous materials.

  6. Effects of Phenobarbital on Expression of UDP-Glucuronosyltransferase 1a6 and 1a7 in Rat Brain.

    PubMed

    Sakakibara, Yukiko; Katoh, Miki; Kondo, Yuya; Nadai, Masayuki

    2016-03-01

    UDP-glucuronosyltransferase (UGT), a phase II drug-metabolizing enzyme, is expressed in the brain and can catalyze glucuronidation of endogenous and exogenous substrates in the brain. Thus, changes in UGT1A expression could affect homeostasis and drug efficacy. Phenobarbital (PB), a typical inducer of drug-metabolizing enzymes, has been reported to induce oxidative stress and epigenetic changes, which could alter UGT expression in the brain. Here, we aimed to clarify the effects of PB on Ugt1a6 and Ugt1a7 gene expression in rat brains. Sprague-Dawley rats were treated intraperitoneally with PB (80 mg/kg), once daily for 7 days. Ugt1a6 and Ugt1a7 mRNA expression levels were increased in the striatum and thalamus (Ugt1a6, 3.0- and 2.9-fold, respectively; Ugt1a7, 2.6- and 2.6-fold, respectively). Acetaminophen glucuronidation was also increased in the medulla oblongata and thalamus by 1.8- and 1.2-fold, respectively. The induction rates within different brain regions were correlated with Ugt1a6 and Ugt1a7 mRNA expression, and the degree of induction also correlated with that of NF-E2-related factor-2 mRNA. Measurement of oxidative stress markers suggested that PB induced oxidative stress in brain regions in which Ugt1a6 and Ugt1a7 mRNAs were increased. Moreover, histone modifications were altered by PB treatment, resulting in increased histone H3 lysine 4 trimethylation in the striatum and thalamus and decreased histone H3 lysine 9 trimethylation in the thalamus. These results suggested that oxidative stress and histone modifications may promote transcriptional activation of Ugt1a6 and Ugt1a7 genes. In summary, Ugt1a6 and Ugt1a7 mRNA levels were increased by PB treatment, which may alter pharmacokinetics in the brain.

  7. eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference

    NASA Astrophysics Data System (ADS)

    Yi, Tingfang; Arthanari, Haribabu; Akabayov, Barak; Song, Huaidong; Papadopoulos, Evangelos; Qi, Hank H.; Jedrychowski, Mark; Güttler, Thomas; Guo, Cuicui; Luna, Rafael E.; Gygi, Steven P.; Huang, Stephen A.; Wagner, Gerhard

    2015-05-01

    MicroRNA (miRNA) biogenesis and miRNA-guided RNA interference (RNAi) are essential for gene expression in eukaryotes. Here we report that translation initiation factor eIF1A directly interacts with Ago2 and promotes Ago2 activities in RNAi and miR-451 biogenesis. Biochemical and NMR analyses demonstrate that eIF1A binds to the MID domain of Ago2 and this interaction does not impair translation initiation. Alanine mutation of the Ago2-facing Lys56 in eIF1A impairs RNAi activities in human cells and zebrafish. The eIF1A-Ago2 assembly facilitates Dicer-independent biogenesis of miR-451, which mediates erythrocyte maturation. Human eIF1A (heIF1A), but not heIF1A(K56A), rescues the erythrocyte maturation delay in eif1axb knockdown zebrafish. Consistently, miR-451 partly compensates erythrocyte maturation defects in zebrafish with eif1axb knockdown and eIF1A(K56A) expression, supporting a role of eIF1A in miRNA-451 biogenesis in this model. Our results suggest that eIF1A is a novel component of the Ago2-centred RNA-induced silencing complexes (RISCs) and augments Ago2-dependent RNAi and miRNA biogenesis.

  8. JPL preferred parts list: Reliable electronic components

    NASA Technical Reports Server (NTRS)

    Covey, R. E.; Scott, W. R.; Hess, L. M.; Steffy, T. G.; Stott, F. R.

    1982-01-01

    The JPL Preferred Parts List was prepared to provide a basis for selection of electronic parts for JPL spacecraft programs. Supporting tests for the listed parts were designed to comply with specific spacecraft environmental requirements. The list tabulates the electronic, magnetic, and electromechanical parts applicable to all JPL electronic equipment wherein reliability is a major concern. The parts listed are revelant to equipment supplied by subcontractors as well as fabricated at the laboratory.

  9. Management of a coordinated parts program

    NASA Technical Reports Server (NTRS)

    Krishnan, G. S.

    1997-01-01

    The organization of the management of a parts program is discussed. The organizational structure faced by the parts manager and the advantages and disadvantages of managing a coordinated parts program are analyzed. The reliable operation of an instrument is the key to the success of the mission, together with the management of the parts program. The analysis led to the conclusion that the setting up of the decision support model will aid the parts manager in the decision making and the process control.

  10. Correcting Part-Time Misconceptions. Policy Watch.

    ERIC Educational Resources Information Center

    Employment Policies Inst., Washington, DC.

    Part-time workers are those working fewer than 35 hours per week. Of the 113 million wage and salary workers in the labor force, only 17 percent are classified as part time. Four of five part-time workers choose to work part-time rather than full-time. The 3.8 million involuntary part-time workers constitute only 3.4 percent of the work force.…

  11. Beluga whale liver microsomal cytochrome P4501A (CYP1A) enzymes

    SciTech Connect

    Bullock, P.L.; Addison, R.; Lockhart, L.; Metner, D.

    1995-12-31

    Beluga whale (Delphinapterus leucas) liver from the Canadian arctic was analyzed for the presence of CYP1A enzymes, as part of current studies on biomarkers for environmental contamination. CYP1A1-associated 7-ethoxyresorufin O-dealkylase activity (EROD) varied 13 fold among sixteen male whale liver microsomal samples and 31 fold among five females. Similarly, the rate of 7-methoxyresorufin O-dealkylation (MROD) varied 7 fold and 3 fold in microsomal samples from males and females, respectively. Furthermore, 7-pentoxyresorufin O-dealkylase activity (PROD) varied 10 fold in both sexes. None of these enzyme activities were sexually differentiated, and EROD and MROD were inhibited by {alpha}-naphthoflavone. There was very good correlation between EROD and MROD (r{sup 2} = .894), EROD and PROD (r{sup 2} = .909), but MROD and PROD were not as well correlated (r{sup 2} = 785). On Western immunoblots, a single band was recognized in Beluga whale liver microsomes by a polygonal antibody raised against an oligopeptide related to trout CYP1A1. This antibody also recognized purified rat CYP1A1 (56 kDa) and stained only one band (56 kDa) in liver microsomes isolated from male rats treated with {beta}-naphthoflavone. The interindividual variation in EROD paralleled differences in the amount of whale liver microsomal protein that cross-reacted with the anti-peptide antibody. The results suggest that Beluga whale liver contains at least one CYP1A enzyme which catalyzes the 0-dealkylation of 7-ethoxy, 7-methoxy and 7-pentoxyresorufin and has a molecular weight less than that of rat CYP1A1, but similar to rat CYP1A2 (52 kDa).

  12. DYRK1A kinase inhibitors with emphasis on cancer.

    PubMed

    Ionescu, A; Dufrasne, F; Gelbcke, M; Jabin, I; Kiss, R; Lamoral-Theys, D

    2012-11-01

    Various types of cancers (including gliomas, melanomas, and esophageal, pancreas and non-small-cell lung cancers) display intrinsic resistance to pro-apoptotic stimuli, such as conventional chemotherapy and radiotherapy, and/or the activation of a multidrug resistance phenotype, which are major barriers to effective treatment and lead to poor patient prognosis. The DYRK1A kinase is directly implicated in the resistance of cancer cells to pro-apoptotic stimuli and drives several pathways that enhance proliferation, migration, and the reduction of cell death, leading to very aggressive biological behavior in cancer cell populations. The DYRK1A kinase is also implicated in neurological diseases and in neoangiogenic processes. Thus, the DYRK1A kinase is of great interest for both cancer and neuroscience research. During the last decade, numerous compounds that inhibit DYRK1A have been synthesized. The present review discusses the available molecules known to interfere with DYRK1A activity and the implications of DYRK1A in cancer and other diseases and serves as a rational analysis for researchers who aim to improve the anti-DYRK1A activity of currently available compounds. PMID:23016545

  13. Impact of Dyrk1A level on alcohol metabolism.

    PubMed

    Renon, Marjorie; Legrand, Béatrice; Blanc, Etienne; Daubigney, Fabrice; Bokobza, Cindy; Mortreux, Marie; Paul, Jean-Louis; Delabar, Jean-Maurice; Rouach, Hélène; Andreau, Karine; Janel, Nathalie

    2016-09-01

    Alcoholic liver diseases arise from complex phenotypes involving many genetic factors. It is quite common to find hyperhomocysteinemia in chronic alcoholic liver diseases, mainly due to deregulation of hepatic homocysteine metabolism. Dyrk1A, involved in homocysteine metabolism at different crossroads, is decreased in liver of hyperhomocysteinemic mice. Here, we hypothesized that Dyrk1A contributes to alcohol-induced hepatic impairment in mice. Control, hyperhomocysteinemic and mice overexpressing Dyrk1A were fed using a Lieber-DeCarli liquid diet with or without ethanol (5% v/v ethanol) for one month, and liver histological examination and liver biochemical function tests were performed. Plasma alanine aminotransferase and homocysteine levels were significantly decreased in mice overexpressing Dyrk1A compared to control mice with or without alcohol administration. On the contrary, the mean plasma alanine aminotransferase and homocysteine levels were significantly higher in hyperhomocysteinemic mice than that of control mice after alcohol administration. Paraoxonase 1 and CYP2E1, two phase I xenobiotic metabolizing enzymes, were found increased in the three groups of mice after alcohol administration. However, NQO1, a phase II enzyme, was only found increased in hyperhomocysteinemic mice after alcohol exposure, suggesting a greater effect of alcohol in liver of hyperhomocysteinemic mice. We observed positive correlations between hepatic alcohol dehydrogenase activity, Dyrk1A and ADH4 protein levels. Importantly, a deleterious effect of alcohol consumption on hepatic Dyrk1A protein level was found. Our study reveals on the one hand a role of Dyrk1A in ethanol metabolism and on the other hand a deleterious effect of alcohol administration on hepatic Dyrk1A level.

  14. Impact of Dyrk1A level on alcohol metabolism.

    PubMed

    Renon, Marjorie; Legrand, Béatrice; Blanc, Etienne; Daubigney, Fabrice; Bokobza, Cindy; Mortreux, Marie; Paul, Jean-Louis; Delabar, Jean-Maurice; Rouach, Hélène; Andreau, Karine; Janel, Nathalie

    2016-09-01

    Alcoholic liver diseases arise from complex phenotypes involving many genetic factors. It is quite common to find hyperhomocysteinemia in chronic alcoholic liver diseases, mainly due to deregulation of hepatic homocysteine metabolism. Dyrk1A, involved in homocysteine metabolism at different crossroads, is decreased in liver of hyperhomocysteinemic mice. Here, we hypothesized that Dyrk1A contributes to alcohol-induced hepatic impairment in mice. Control, hyperhomocysteinemic and mice overexpressing Dyrk1A were fed using a Lieber-DeCarli liquid diet with or without ethanol (5% v/v ethanol) for one month, and liver histological examination and liver biochemical function tests were performed. Plasma alanine aminotransferase and homocysteine levels were significantly decreased in mice overexpressing Dyrk1A compared to control mice with or without alcohol administration. On the contrary, the mean plasma alanine aminotransferase and homocysteine levels were significantly higher in hyperhomocysteinemic mice than that of control mice after alcohol administration. Paraoxonase 1 and CYP2E1, two phase I xenobiotic metabolizing enzymes, were found increased in the three groups of mice after alcohol administration. However, NQO1, a phase II enzyme, was only found increased in hyperhomocysteinemic mice after alcohol exposure, suggesting a greater effect of alcohol in liver of hyperhomocysteinemic mice. We observed positive correlations between hepatic alcohol dehydrogenase activity, Dyrk1A and ADH4 protein levels. Importantly, a deleterious effect of alcohol consumption on hepatic Dyrk1A protein level was found. Our study reveals on the one hand a role of Dyrk1A in ethanol metabolism and on the other hand a deleterious effect of alcohol administration on hepatic Dyrk1A level. PMID:27216978

  15. 49 CFR Appendix A to Part 232 - Schedule of Civil Penalties 1

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 4 2014-10-01 2014-10-01 false Schedule of Civil Penalties 1 A Appendix A to Part... TRAINS AND EQUIPMENT; END-OF-TRAIN DEVICES Pt. 232, App. A Appendix A to Part 232—Schedule of Civil...) Improper movement, general (1) (1) (11) Failure to make determinations and provide notification of en...

  16. 49 CFR Appendix A to Part 232 - Schedule of Civil Penalties 1

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 4 2013-10-01 2013-10-01 false Schedule of Civil Penalties 1 A Appendix A to Part... TRAINS AND EQUIPMENT; END-OF-TRAIN DEVICES Pt. 232, App. A Appendix A to Part 232—Schedule of Civil...) Improper movement, general (1) (1) (11) Failure to make determinations and provide notification of en...

  17. 49 CFR Appendix A to Part 232 - Schedule of Civil Penalties 1

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 4 2012-10-01 2012-10-01 false Schedule of Civil Penalties 1 A Appendix A to Part... TRAINS AND EQUIPMENT; END-OF-TRAIN DEVICES Pt. 232, App. A Appendix A to Part 232—Schedule of Civil...) Improper movement, general (1) (1) (11) Failure to make determinations and provide notification of en...

  18. 15 CFR 740.10 - License Exception Servicing and replacement of parts and equipment (RPL).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... the ITAR, i.e., described on the United States Munitions List (22 CFR 121.1). (a) Parts, components... software imported from Country Group D:1 except the People's Republic of China (PRC). Commodities and... of China (PRC)) (see Supplement No. 1 to this part) that are sent to the United States or a...

  19. Sports Concussion Management: part II.

    PubMed

    Terrell, Thomas R; Cox, Conrad B; Bielak, Ken; Casmus, Robert; Laskowitz, Daniel; Nichols, Gregory

    2014-02-01

    Millions of concussions occur every year in the United States. The public interest in concussion has increased after a number of high-profile deaths in high school athletes from sports-related head trauma and in some professional athletes from chronic traumatic encephalopathy. One of the most active areas of research in sports medicine during the last decade has been the evaluation and management of concussion. In this second article of a two-part series, we provide an overview of the latest scientific advances in concussion research. This overview includes an update on the pathobiological changes that occur during concussion and the results of biomechanical studies. In addition, to aid the practicing clinician, we review the literature on proven and currently studied concussion risk factors, including a history of concussion, fatigue, and age. Genetic polymorphisms and biomarkers may provide risk-prediction capability, but at present the research remains inconclusive. Diffusion tensor imaging and functional magnetic resonance imaging are promising technologies that reveal more sophisticated data about the impact of concussion on the brain. We review the existing literature on the application of these neuroimaging modalities to sports concussion. An update from the Fourth International Conference on Concussion in Sport, with highlights of new recommendations, and the presentation of the third edition of the Sports Concussion Assessment Tool to evaluate acute concussion, concludes our review.

  20. Commercial Parts Technology Qualification Processes

    NASA Technical Reports Server (NTRS)

    Cooper, Mark S.

    2013-01-01

    Many high-reliability systems, including space systems, use selected commercial parts (including Plastic Encapsulated Microelectronics or PEMs) for unique functionality, small size, low weight, high mechanical shock resistance, and other factors. Predominantly this usage is subjected to certain 100% tests (typically called screens) and certain destructive tests usually (but not always) performed on the flight lot (typically called qualification tests). Frequently used approaches include those documented in EEE-INST-002 and JPL DocID62212 (which are sometimes modified by the particular aerospace space systems manufacturer). In this study, approaches from these documents and several space systems manufacturers are compared to approaches from a launch systems manufacturer (SpaceX), an implantable medical electronics manufacturer (Medtronics), and a high-reliability transport system process (automotive systems). In the conclusions section, these processes are outlined for all of these cases and presented in tabular form. Then some simple comparisons are made. In this introduction section, the PEM technology qualification process is described, as documented in EEE-INST-002 (written by the Goddard Space Flight Center, GSFC), as well as the somewhat modified approach employed at the Jet Propulsion Laboratory (JPL). Approaches used at several major NASA contractors are also described

  1. 49 CFR Figure 1a to Subpart B of... - Example of Location of Rescue Access Windows-§ 238.114

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Example of Location of Rescue Access Windows-§ 238.114 1A Figure 1A to Subpart B of Part 238 Transportation Other Regulations Relating to... B of Part 238—Example of Location of Rescue Access Windows—§ 238.114 ER01FE08.002...

  2. 49 CFR Figure 1a to Subpart B of... - Example of Location of Rescue Access Windows-§ 238.114

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 4 2011-10-01 2011-10-01 false Example of Location of Rescue Access Windows-§ 238.114 1A Figure 1A to Subpart B of Part 238 Transportation Other Regulations Relating to... B of Part 238—Example of Location of Rescue Access Windows—§ 238.114 ER01FE08.002...

  3. 49 CFR Figure 1a to Subpart B of... - Example of Location of Rescue Access Windows-§ 238.114

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 4 2014-10-01 2014-10-01 false Example of Location of Rescue Access Windows-§ 238.114 1A Figure 1A to Subpart B of Part 238 Transportation Other Regulations Relating to... B of Part 238—Example of Location of Rescue Access Windows—§ 238.114 ER01FE08.002 [73 FR 6403,...

  4. 49 CFR Figure 1a to Subpart B of... - Example of Location of Rescue Access Windows-§ 238.114

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 4 2013-10-01 2013-10-01 false Example of Location of Rescue Access Windows-§ 238.114 1A Figure 1A to Subpart B of Part 238 Transportation Other Regulations Relating to... B of Part 238—Example of Location of Rescue Access Windows—§ 238.114 ER01FE08.002...

  5. 1. A LONG VIEW, LOOKING NORTH FROM THE WEST BANK ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. A LONG VIEW, LOOKING NORTH FROM THE WEST BANK OF LITTLE WALNUT CREEK TOWARD THE SOUTH (DOWNSTREAM) SIDE OF THE BRIDGE - Putnam County Bridge No. 111, Spanning Little Walnut Creek on County Road 50, Greencastle, Putnam County, IN

  6. Recurrent PRKAR1A mutation in acrodysostosis with hormone resistance.

    PubMed

    Linglart, Agnès; Menguy, Christine; Couvineau, Alain; Auzan, Colette; Gunes, Yasemin; Cancel, Mathilde; Motte, Emmanuelle; Pinto, Graziella; Chanson, Philippe; Bougnères, Pierre; Clauser, Eric; Silve, Caroline

    2011-06-01

    The skeletal dysplasia characteristic of acrodysostosis resembles the Albright's hereditary osteodystrophy seen in patients with pseudohypoparathyroidism type 1a, but defects in the α-stimulatory subunit of the G-protein (GNAS), the cause of pseudohypoparathyroidism type 1a, are not present in patients with acrodysostosis. We report a germ-line mutation in the gene encoding PRKAR1A, the cyclic AMP (cAMP)-dependent regulatory subunit of protein kinase A, in three unrelated patients with acrodysostosis and resistance to multiple hormones. The mutated subunit impairs the protein kinase A response to stimulation by cAMP; this explains our patients' hormone resistance and the similarities of their skeletal abnormalities with those observed in patients with pseudohypoparathyroidism type 1a. PMID:21651393

  7. Distinct neurological disorders with ATP1A3 mutations

    PubMed Central

    Heinzen, Erin L.; Arzimanoglou, Alexis; Brashear, Allison; Clapcote, Steven J.; Gurrieri, Fiorella; Goldstein, David B.; Jóhannesson, Sigurður H.; Mikati, Mohamad A.; Neville, Brian; Nicole, Sophie; Ozelius, Laurie J.; Poulsen, Hanne; Schyns, Tsveta; Sweadner, Kathleen J.; van den Maagdenberg, Arn; Vilsen, Bente

    2014-01-01

    Genetic research has shown that mutations that modify the protein-coding sequence of ATP1A3, the gene encoding the α3 subunit of Na+/K+-ATPase, cause both rapid-onset dystonia parkinsonism and alternating hemiplegia of childhood. These discoveries link two clinically distinct neurological diseases to the same gene, however, ATP1A3 mutations are, with one exception, disease-specific. Although the exact mechanism of how these mutations lead to disease is still unknown, much knowledge has been gained about functional consequences of ATP1A3 mutations using a range of in vitro and animal model systems, and the role of Na+/K+-ATPases in the brain. Researchers and clinicians are attempting to further characterise neurological manifestations associated with mutations in ATP1A3, and to build on the existing molecular knowledge to understand how specific mutations can lead to different diseases. PMID:24739246

  8. 1. A BRICK AND CONCRETE FAN HOUSING ADJACENT TO ONE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. A BRICK AND CONCRETE FAN HOUSING ADJACENT TO ONE OF THE ADIT OPENINGS (VIEW TO THE NORTH). - Foster Gulch Mine, Fan Housing, Bear Creek 1 mile Southwest of Town of Bear Creek, Red Lodge, Carbon County, MT

  9. Distinct neurological disorders with ATP1A3 mutations.

    PubMed

    Heinzen, Erin L; Arzimanoglou, Alexis; Brashear, Allison; Clapcote, Steven J; Gurrieri, Fiorella; Goldstein, David B; Jóhannesson, Sigurður H; Mikati, Mohamad A; Neville, Brian; Nicole, Sophie; Ozelius, Laurie J; Poulsen, Hanne; Schyns, Tsveta; Sweadner, Kathleen J; van den Maagdenberg, Arn; Vilsen, Bente

    2014-05-01

    Genetic research has shown that mutations that modify the protein-coding sequence of ATP1A3, the gene encoding the α3 subunit of Na(+)/K(+)-ATPase, cause both rapid-onset dystonia parkinsonism and alternating hemiplegia of childhood. These discoveries link two clinically distinct neurological diseases to the same gene, however, ATP1A3 mutations are, with one exception, disease-specific. Although the exact mechanism of how these mutations lead to disease is still unknown, much knowledge has been gained about functional consequences of ATP1A3 mutations using a range of in-vitro and animal model systems, and the role of Na(+)/K(+)-ATPases in the brain. Researchers and clinicians are attempting to further characterise neurological manifestations associated with mutations in ATP1A3, and to build on the existing molecular knowledge to understand how specific mutations can lead to different diseases. PMID:24739246

  10. The Algorithm Theoretical Basis Document for Level 1A Processing

    NASA Technical Reports Server (NTRS)

    Jester, Peggy L.; Hancock, David W., III

    2012-01-01

    The first process of the Geoscience Laser Altimeter System (GLAS) Science Algorithm Software converts the Level 0 data into the Level 1A Data Products. The Level 1A Data Products are the time ordered instrument data converted from counts to engineering units. This document defines the equations that convert the raw instrument data into engineering units. Required scale factors, bias values, and coefficients are defined in this document. Additionally, required quality assurance and browse products are defined in this document.

  11. [Antidepressants, stressors and the serotonin 1A receptor].

    PubMed

    Kirilly, Eszter; Gonda, Xénia; Bagdy, György

    2015-06-01

    5-HT(1A) receptor is a receptor of surprises. Buspirone, an anxiolytic drug with a then yet unidentified mechanism of action had been marketed for years when it was discovered that it is a 5-HT(1A) partial agonist. Several more years had to pass before it was accepted that this receptor plays the key role in the action mechanism of buspirone. This was followed by further surprises. It was discovered that in spite of its anxiolytic effect buspirone activates the hypothalamic-pituitary-adrenal (HPA) stress axis, furthermore, it increases peripheral noradrenaline and adrenaline concentration via a central mechanism. Thus activation of this receptor leads to ACTH/corticosterone and catecholamine release and also increases beta-endorphine, oxytocin and prolactin secretion while decreasing body temperature, increasing food uptake, eliciting characteristic behavioural responses in rodents and also playing a role in the development of certain types of epilepsy. Human genetic studies revealed the role of 5-HT(1A) receptors in cognitive processes playing a role in the development of depression such as impulsiveness or response to environmental stress. This exceptionally wide spectrum of effects is attributable to the presence of 5-HT1A receptors in serotonergic as well as other, for example glutamatergic, cholinergic, dopaminergic and noradrenergic neurons. The majority of the effects of 5-HT(1A) receptors is manifested via the mediation of Gi proteins through the hyperpolarisation or inhibition of the neuron carrying the receptor. 5-HT(1A) receptors on serotonergic neurons can be found in the somatodendritic area and play a significant role in delaying the effects of antidepressants which is an obvious disadvantage. Therefore the newest serotonergic antidepressants including vilazodone and vortioxetine have been designed to possess 5-HT(1A) receptor partial agonist properties. In the present paper we focus primarily on the role of 5-HT(1A) receptors in stress and

  12. Paresev 1-A and tow plane with crew and pilot

    NASA Technical Reports Server (NTRS)

    1962-01-01

    With the the Paresev 1-A and the 450-hp Stearman sport Biplane as a backdrop the Pilot and crew pose for this picture in 1962. Starting at left: On the motorcycle is Walter Whiteside, in the Paresev 1-A is test pilot Milton Thompson, Frank Fedor, Richard Klein, Victor Horton, Tom Kelly, Jr., Fred Harris, owner of the Stearman, John Orahood, and Gary Layton.

  13. 47 CFR 80.1091 - Ship radio equipment-Sea areas A1, A2, and A3.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... accordance with 5 U.S.C. 552(a) and 1 CFR part 51. Copies of these standards can be inspected at the Federal... 47 Telecommunication 5 2011-10-01 2011-10-01 false Ship radio equipment-Sea areas A1, A2, and A3... SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Global Maritime Distress and Safety System...

  14. Mutations in the Microtubule-Associated Protein 1A (Map1a) Gene Cause Purkinje Cell Degeneration

    PubMed Central

    Liu, Ye; Lee, Jeong Woong

    2015-01-01

    The structural microtubule-associated proteins (MAPs) are critical for the organization of neuronal microtubules (MTs). Microtubule-associated protein 1A (MAP1A) is one of the most abundantly expressed MAPs in the mammalian brain. However, its in vivo function remains largely unknown. Here we describe a spontaneous mouse mutation, nm2719, which causes tremors, ataxia, and loss of cerebellar Purkinje neurons in aged homozygous mice. The nm2719 mutation disrupts the Map1a gene. We show that targeted deletion of mouse Map1a gene leads to similar neurodegenerative defects. Before neuron death, Map1a mutant Purkinje cells exhibited abnormal focal swellings of dendritic shafts and disruptions in axon initial segment (AIS) morphology. Furthermore, the MT network was reduced in the somatodendritic and AIS compartments, and both the heavy and light chains of MAP1B, another brain-enriched MAP, was aberrantly distributed in the soma and dendrites of mutant Purkinje cells. MAP1A has been reported to bind to the membrane-associated guanylate kinase (MAGUK) scaffolding proteins, as well as to MTs. Indeed, PSD-93, the MAGUK specifically enriched in Purkinje cells, was reduced in Map1a−/− Purkinje cells. These results demonstrate that MAP1A functions to maintain both the neuronal MT network and the level of PSD-93 in neurons of the mammalian brain. PMID:25788676

  15. Range Imaging without Moving Parts

    NASA Technical Reports Server (NTRS)

    Blair, J. Bryan; Scott, V. Stanley, III; Ramos-Izquierdo, Luis

    2008-01-01

    Range-imaging instruments of a type now under development are intended to generate the equivalent of three-dimensional images from measurements of the round-trip times of flight of laser pulses along known directions. These instruments could also provide information on characteristics of targets, including roughnesses and reflectivities of surfaces and optical densities of such semi-solid objects as trees and clouds. Unlike in prior range-imaging instruments based on times of flight along known directions, there would be no moving parts; aiming of the laser beams along the known directions would not be accomplished by mechanical scanning of mirrors, prisms, or other optical components. Instead, aiming would be accomplished by using solid-state devices to switch input and output beams along different fiber-optic paths. Because of the lack of moving parts, these instruments could be extraordinarily reliable, rugged, and long-lasting. An instrument of this type would include an optical transmitter that would send out a laser pulse along a chosen direction to a target. An optical receiver coaligned with the transmitter would measure the temporally varying intensity of laser light reflected from the target to determine the distance and surface characteristics of the target. The transmitter would be a combination of devices for generating precise directional laser illumination. It would include a pulsed laser, the output of which would be coupled into a fiber-optic cable with a fan-out and solid-state optical switches that would enable switching of the laser beam onto one or more optical fibers terminated at known locations in an array on a face at the focal plane of a telescope. The array would be imaged by the telescope onto the target space. The receiver optical system could share the aforementioned telescope with the transmitter or could include a separate telescope aimed in the same direction as that of the transmitting telescope. In either case, light reflected

  16. PRKAR1A and the evolution of pituitary tumors.

    PubMed

    Kirschner, Lawrence S

    2010-09-15

    Carney complex (CNC) is an inherited tumor predisposition associated with pituitary tumors, including GH-producing pituitary adenomas and rare reports of prolactinomas. This disease is caused by mutations in PRKAR1A, which encodes the type 1A regulatory subunit of the cAMP-dependent protein kinase, PKA. Loss of PRKAR1A causes enhanced PKA signaling, which leads to pituitary tumorigenesis. Mutations in the gene have not been detected in sporadic pituitary tumors, but there is some data to suggest that non-genomic mechanisms may cause loss of protein expression. Unlike CNC patients, mice heterozygous for Prkar1a mutations do not develop pituitary tumors, although complete knockout of the gene in the Pit1 lineage of the pituitary produces GH-secreting pituitary adenomas. These data indicate that complete loss of Prkar1a/PRKAR1A is able to cause pituitary tumors in mice and men. The pattern of tumors is likely related to the signaling pathways employed in specific pituitary cell types. PMID:20451576

  17. Mutations in PTF1A cause pancreatic and cerebellar agenesis.

    PubMed

    Sellick, Gabrielle S; Barker, Karen T; Stolte-Dijkstra, Irene; Fleischmann, Christina; Coleman, Richard J; Garrett, Christine; Gloyn, Anna L; Edghill, Emma L; Hattersley, Andrew T; Wellauer, Peter K; Goodwin, Graham; Houlston, Richard S

    2004-12-01

    Individuals with permanent neonatal diabetes mellitus usually present within the first three months of life and require insulin treatment. We recently identified a locus on chromosome 10p13-p12.1 involved in permanent neonatal diabetes mellitus associated with pancreatic and cerebellar agenesis in a genome-wide linkage search of a consanguineous Pakistani family. Here we report the further linkage analysis of this family and a second family of Northern European descent segregating an identical phenotype. Positional cloning identified the mutations 705insG and C886T in the gene PTF1A, encoding pancreas transcription factor 1alpha, as disease-causing sequence changes. Both mutations cause truncation of the expressed PTF1A protein C-terminal to the basic-helix-loop-helix domain. Reporter-gene studies using a minimal PTF1A deletion mutant indicate that the deleted region defines a new domain that is crucial for the function of this protein. PTF1A is known to have a role in mammalian pancreatic development, and the clinical phenotype of the affected individuals implicated the protein as a key regulator of cerebellar neurogenesis. The essential role of PTF1A in normal cerebellar development was confirmed by detailed neuropathological analysis of Ptf1a(-/-) mice. PMID:15543146

  18. [First part: the intestinal microbiota].

    PubMed

    Capurso, Lucio

    2016-06-01

    The human gastrointestinal tract contains a large number of commensal (non pathogenic) and pathogenic microbial species that have co-evolved with the human genome and differ in composition and function based on their location, as well as age, sex, race/ethnicity, and diet of their host and we can in fact consider the human body as a mix of human and bacterial cells. It is now evident that the large intestine is much more than an organ for waste material and absorption of water, salts and drugs, and indeed has a very important impact on human health, for a major part related to the specific composition of the complex microbial community in the colon. In man, the large gut receives material from the ileum which has already been digested and the contents are then mixed and retained for 6-12 hours in the caecum and right colon. Thus, the large intestine is an open system, with nutrients flowing in the caecum, and bacteria, their metabolic products, and undigested foodstuffs being excreted as faeces. The anaerobic brakdown of carbohydrate and protein by bacteria is known conventionally as fermentation. In man the major end products are the short-chain fatty acids (SCFA) acetate, propionate, butirate, the gases H2 and CO2, ammonia, amines, phenols and energy, which the bacteria use for growth and the maintenance of cellular function. The microbiota is also an important factor in the development of the immune response. The interaction between the gastrointestinal tract and resident microbiota is well balanced in healthy individuals, but its breakdown can lead to intestinal and extraintestinal disease. PMID:27362717

  19. Plantaricin IIA-1A5 from Lactobacillus plantarum IIA-1A5 displays bactericidal activity against Staphylococcus aureus.

    PubMed

    Arief, I Isnafia; Budiman, C; Jenie, B Sri Laksmi; Andreas, E; Yuneni, A

    2015-01-01

    Plantaricin IIA-1A5 is a bacteriocin produced by Lactobacillus plantarum IIA-1A5 isolated from Indonesian beef. This research aimed to identify the genes involved in plantaricin IIA-1A5 production and examine its mode of action against Staphylococcus aureus. It has been reported that a bacteriocin structural gene, plnW, is present in genome of L. plantarum IIA-1A5. Here, we reported the presence of additional genes responsible for plantaricin precursor (plnA and plnEF) and a gene encoding the quorum sensor of histidine kinase (plnB). It indicates that genes involved in production of plantaricin IIA-1A5 are organized in at least two bacteriocin operons (plnABCD, plnEFI) and a structural plnW gene. Purified plantaricin IIA-1A5 yielded a single band in SDS-PAGE with apparent size of 6.4 kDa. Amino acid composition of purified plantaricin IIA-1A5 was mainly composed of cationic glutamic acid and cysteine that allowed the formation of disulphide bonds, suggesting plantaricin IIA-1A5 belongs to the pediocin-subclass of class II bacteriocins. Plantaricin IIA-1A5 displayed remarkable antibacterial activity against S. aureus, which was initiated by the adsorption of plantaricin IIA-1A5 onto the cell membrane of S. aureus. The adsorption is hypothesised to be facilitated by non-ionic interactions as it is reduced by the presence of organic solvents or detergents. This adsorption promoted leakage of cellular metabolites through the cell membrane of S. aureus, as indicated by the release of genetic and proteinaceous material of S. aureus observed at 260 and 280 nm, respectively. The leakage also promoted the release of divalent (Ca(2+), Mg(2+)) and monovalent (K(+)) cations. The release of these intracellular components might be due to pores formed in the cell membrane of S. aureus by plantaricin IIA-1A5 as shown by scanning electron microscopy. Altogether, the mode of action of plantaricin IIA-1A5 against S. aureus seems to be bactericidal as indicated by lysis of the cell

  20. 34 CFR Appendix E to Part 300 - Index for IDEA-Part B Regulations (34 CFR Part 300)

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Index for IDEA-Part B Regulations (34 CFR Part 300) E... B Regulations (34 CFR Part 300) ACCESS TO • Access rights (Parents) 300.613. • Assistive technology....115. ALTERNATIVE STRATEGIES to meet transition objectives 300.324(c)(1). AMENDMENTS • To LEA...

  1. 34 CFR Appendix E to Part 300 - Index for IDEA-Part B Regulations (34 CFR Part 300)

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Index for IDEA-Part B Regulations (34 CFR Part 300) E... B Regulations (34 CFR Part 300) ACCESS TO • Access rights (Parents) 300.613. • Assistive technology... services 300.300(b). CONSENT (J-Z) • Not required: Ο Before administering a test or other evaluation to...

  2. 34 CFR Appendix E to Part 300 - Index for IDEA-Part B Regulations (34 CFR Part 300)

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Index for IDEA-Part B Regulations (34 CFR Part 300) E... B Regulations (34 CFR Part 300) ACCESS TO • Access rights (Parents) 300.613. • Assistive technology... services 300.300(b). CONSENT (J-Z) • Not required: Ο Before administering a test or other evaluation to...

  3. 34 CFR Appendix E to Part 300 - Index for IDEA-Part B Regulations (34 CFR Part 300)

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Index for IDEA-Part B Regulations (34 CFR Part 300) E... B Regulations (34 CFR Part 300) ACCESS TO • Access rights (Parents) 300.613. • Assistive technology... assessments 300.320(a)(6)(i). • State level activities in support of 300.704(b)(4)(x). ACT (Definition)...

  4. 34 CFR Appendix E to Part 300 - Index for IDEA-Part B Regulations (34 CFR Part 300)

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Index for IDEA-Part B Regulations (34 CFR Part 300) E... B Regulations (34 CFR Part 300) ACCESS TO • Access rights (Parents) 300.613. • Assistive technology... assessments 300.320(a)(6)(i). • State level activities in support of 300.704(b)(4)(x). ACT (Definition)...

  5. The pathogenic potential of Yersinia enterocolitica 1A.

    PubMed

    Batzilla, Julia; Heesemann, Juergen; Rakin, Alexander

    2011-11-01

    Yersinia enterocolitica 1A strains are generally considered apathogenic. However, besides environmental sources, foods and animals, they are repeatedly isolated from patients with gastrointestinal symptoms typical to those evoked by Yersinia of the virulent 1B and 2-4 biotypes. Also, at least 2 gastrointestinal outbreaks associated with 1A strains have been reported. There is a general controversy concerning the pathogenic potential of 1A isolates of clinical and non-clinical origin. To address the 1A puzzle, we have determined the genome sequences of 2 1A strains, a nosocomial O:5 and environmental O:36 isolates, and compared them to each other and to O:8/1B and O:3/4 representatives of the virulent serobiotypes. 1A isolates have mosaic genomes and share genes both with serobiotypes O:8/1B and O:3/4 that implies their common descent. Besides the pYV virulence plasmid, 1A strains lack the classical virulence markers, like the Ail adhesin, the YstA enterotoxin, and the virulence-associated protein C. However, they still possess genes encoding such known and suspect virulence-associated determinants like the YstB enterotoxin, the InvA invasin, the mucoid Yersinia factor MyfA, and the enterochelin utilisation fepBDGC/fepA/fes gene cluster. In contrast to previous studies, we have found that the strains of the 1A group possess the MyfA antigen although with limited similarity to the highly conserved MyfA in the virulent serobiotypes. In turn, the MyfB chaperone coevolved with the MyfA fibrillae, while the MyfC usher retains 90% identity to its MyfC counterparts in O:3/O:8 group. The only notable difference between clinical and non-clinical 1A strains was the presence of a truncated Rtx toxin-like gene cluster and remnants of a P2-like prophage in the hospital O:5 isolate. Taken together, Y. enterocolitica BT 1A group represents opportunistic pathogens whose opportunity to establish infection seems to rely mainly on the state of the host defence system. However

  6. [Modeling of a three-dimensional structure of cytochrome P-450 1A2 and search for its new ligands].

    PubMed

    Belkina, N V; Skvortsov, V S; Ivanov, A S; Archakov, A I

    1998-01-01

    The substances inhibiting cytochrome P450 1A2 (CYP1A2) represent a perspective class of new drugs, which application in clinical practice can become the important part in preventive maintenance in oncology. The present work is devoted to computer modelling of 3-D structure of CYP1A2 and searching of new inhibitors by database mining. The modelling of CYP1A2 was done based on homology with 4 bacterial cytochromes P450 with known 3-D structure. For optimization of CYP1A2 active site structure the models of its complexes with characteristic substrates (caffeine and 7-ethoxyresorufin) were designed. These complexes were optimized by molecular dynamics simulation in water. The models of 24 complexes of CYP1A2 with known ligands with known Kd were designed by means of DockSearch and LeapFrog programs. 3D-QSAR model with good predictive force was created based on these complexes. On a final stage the search of knew CYP1A2 ligands in testing database (more than 23.000 substances from database Maybridge and 112 known CYP1A2 ligands from database Metabolite, MDL) was executed. 680 potential ligands of CYP1A2 with Kd values, comparable with known ones were obtained. This number has included 73 compounds from 112 known ligands, introduced in tested database as the internal control. PMID:9916262

  7. STS-39 AFP-675 CIRRIS-1A in OV-103's payload bay (PLB)

    NASA Technical Reports Server (NTRS)

    1991-01-01

    STS-39's Air Force Program 675 (AFP-675) Uniformly Redundant Array (URA) and Cryogenic Infrared Radiance Instrumentation for Shuttle 1A (CIRRIS-1A) mounted on the experiment support system (ESS) pallet are documented in Discovery's, Orbiter Vehicle (OV) 103's, payload bay (PLB). Part of the ESS pallet power distribution system thermal covering is visible at the bottom of the frame. AFP-675 is a Department of Defense (DOD)-sponsored collection of experiments whose objective is to gather data on the Earth's atmosphere (aurora, Earth limb, and airglow), celestial objects, and the environment in and around the PLB. In the background are the aft PLB bulkhead, the vertical tail, and the orbital maneuvering system (OMS) pods.

  8. E1a promotes c-Myc-dependent replicative stress

    PubMed Central

    Valero, María Llanos; Cimas, Francisco Jose; Arias, Laura; Melgar-Rojas, Pedro; García, Elena; Callejas-Valera, Juan Luis; García-Cano, Jesús; Serrano-Oviedo, Leticia; Ángel de la Cruz-Morcillo, Miguel; Sánchez-Pérez, Isabel; Sánchez-Prieto, Ricardo

    2014-01-01

    The E1a gene from adenovirus is known to be a potent inducer of chemo/radiosensitivity in a wide range of tumors. However, the molecular bases of its radiosensitizer properties are still poorly understood. In an attempt to study this effect, U87MG cells, derived from a radio-resistant tumor as glioblastoma, where infected with lentivirus carrying E1a gene developing an acute sensitivity to ionizing radiation. The induction of radiosensitivity correlated with a marked G2/M phase accumulation and a potent apoptotic response. Our findings demonstrate that c-Myc plays a pivotal role in E1a-associated radiosensitivity through the induction of a replicative stress situation, as our data support by genetic approaches, based in interference and overexpression in U87MG cells. In fact, we present evidence showing that Chk1 is a novel transcriptional target of E1a gene through the effect exerted by this adenoviral protein onto c-Myc. Moreover, c-Myc upregulation also explains the marked phosphorylation of H2AX associated to E1a expression in the absence of DNA damage. Indeed, all these observations were applicable to other experimental models, such as T98G, LN-405 and A172, rendering the same pattern in terms of radiosensitivity, cell cycle distribution, upregulation of Chk1, c-Myc, and phosphorylation pattern of H2AX. In summary, our data propose a novel mechanism to explain how E1a mediates radiosensitivity through the signaling axis E1a→c-Myc→ replicative stress situation. This novel mechanism of E1a-mediated radiosensitivity could be the key to open new possibilities in the current therapy of glioblastoma. PMID:24196438

  9. Part I. Layered Double Hydroxides

    NASA Astrophysics Data System (ADS)

    Dimotakis, Emmanuel Dimitrios

    A new general method for the preparation of well -ordered layered double hydroxides (LDHs), (Mg_ {rm 1-x}Al_{ rm x} (OH)_2) (X^{rm n-}) _{rm n/x}{cdot}yH _2O, interlayered by organic anions has been developed. It is based on the reaction of meixnerite, (Mg_3Al(OH)_8) (OH) cdot2H_2O, with the free acid form of the desired anion--using glycerol as a swelling agent--to yield single crystalline products that are not readily available by conventional synthetic methods. The (Mg_3Al(OH) _8) -adipate and -p-toluenesulfonate derivatives undergo facile ion exchange reactions with Keggin-type (XM_{12}O_ {40}) ^{rm n -} or lacunary (XM_{11 }O_{39}) ^{rm m-} polyoxometalates (POMs) to form well-ordered, microporous pillared derivatives with the highest N_2 BET surface areas reported to date, namely 107 and 155 m^2 /g, respectively. Meixnerite, (Mg_3Al(OH) _8) (OH) cdot2H _2O, has unexpectedly been found to undergo similar ion exchange reactions, in a topotactic way, with retention of the structure of the intercalated POMs. The meixnerite was conveniently prepared for the first time from calcination of (Mg_3Al(OH) _8) (CO_3) _{0.5}{cdot}2H _2O and aqueous hydrolysis of the resulting NaCl-type solid solution. Metal carbonyl clusters have also been examined for ion-exchange (i.e., { (Pt _3(CO)_6) _{rm n}}^ {2-}) in these LDH-precursors. This chemistry is compared with the surface chemistry of (Mg_3 Al(OH)_8) (X^ {rm n-}) _{ rm 1/n}{cdot}2H _2O (X = CO_3^{2 -} or OH^{-}). It has been shown that the surface hydrolysis reaction: CO _3^{2-} + H _2O longrightarrow HCO_3^{-} + OH ^-, causes reductive condensation reactions of neutral carbonyl clusters with the LDH. The reactions were as efficient as with Na metal in solution. In part II of this work, Li-fluorohectorite, has been pillared with titanium polyoxocations derived from the acidic hydrolysis of TiCl_4 or Ti(i-OC_3H_7) _4. Raman spectroscopy of the product indicates that the pillars have a structure analogous to TiO_2(B) phase

  10. Sequencing and characterization of mixed function monooxygenase genes CYP1A1 and CYP1A2 of Mink (Mustela vison) to facilitate study of dioxin-like compounds

    SciTech Connect

    Zhang Xiaowei; Moore, Jeremy N.; Newsted, John L.; Hecker, Markus Zwiernik, Matthew J.; Jones, Paul D.; Bursian, Steven J.

    2009-02-01

    As part of an ongoing effort to understand aryl hydrocarbon receptor (AhR) mediated toxicity in mink, cDNAs encoding for CYP1A1 and the CYP1A2 mixed function monooxygenases were cloned and characterized. In addition, the effects of selected dibenzofurans on the expression of these genes and the presence of their respective proteins (P4501A) were investigated, and then correlated with the catalytic activities of these proteins as measured by ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-deethylase (MROD) activities. The predicted protein sequences for CYP1A1 and CYP1A2 comprise 517 and 512 amino acid residues, respectively. The phylogenetic analysis of the mink CYP1As with protein sequences of other mammals revealed high sequence homology with sea otter, seals and the dog, with amino acid identities ranging from 89 to 95% for CYP1A1 and 81 to 93% for CYP1A2. Since exposure to both 2,3,7,8-Tetrachlorodibenzofuran (TCDF) and 2,3,4,7,8-Pentachlorodibenzofuran (PeCDF) resulted in dose-dependent increases of CYP1A1 mRNA, CYP1A2 mRNA and CYP1A protein levels an underlying AhR-mediated mechanism is suggested. The up-regulation of CYP1A mRNA in liver was more consistent to the sum adipose TEQ concentration than to the liver TEQ concentration in minks treated with TCDF or PeCDF. The result suggested that the hepatic-sequestered fraction of PeCDF was biologically inactive to the induction of CYP1A1 and CYP1A2.

  11. Targeting of eEF1A with Amaryllidaceae isocarbostyrils as a strategy to combat melanomas

    PubMed Central

    Van Goietsenoven, Gwendoline; Hutton, Jenna; Becker, Jean-Paul; Lallemand, Benjamin; Robert, Francis; Lefranc, Florence; Pirker, Christine; Vandenbussche, Guy; Van Antwerpen, Pierre; Evidente, Antonio; Berger, Walter; Prévost, Martine; Pelletier, Jerry; Kiss, Robert; Goss Kinzy, Terri; Kornienko, Alexander; Mathieu, Véronique

    2010-01-01

    Melanomas display poor response rates to adjuvant therapies because of their intrinsic resistance to proapoptotic stimuli. This study indicates that such resistance can be overcome, at least partly, through the targeting of eEF1A elongation factor with narciclasine, an Amaryllidaceae isocarbostyril controlling plant growth. Narciclasine displays IC50 growth inhibitory values between 30–100 nM in melanoma cell lines, irrespective of their levels of resistance to proapoptotic stimuli. Normal noncancerous cell lines are much less affected. At nontoxic doses, narciclasine also significantly improves (P=0.004) the survival of mice bearing metastatic apoptosis-resistant melanoma xenografts in their brain. The eEF1A targeting with narciclasine (50 nM) leads to 1) marked actin cytoskeleton disorganization, resulting in cytokinesis impairment, and 2) protein synthesis impairment (elongation and initiation steps), whereas apoptosis is induced at higher doses only (≥200 nM). In addition to molecular docking validation and identification of potential binding sites, we biochemically confirmed that narciclasine directly binds to human recombinant and yeast-purified eEF1A in a nanomolar range, but not to actin or elongation factor 2, and that 5 nM narciclasine is sufficient to impair eEF1A-related actin bundling activity. eEF1A is thus a potential target to combat melanomas regardless of their apoptosis-sensitivity, and this finding reconciles the pleiotropic cytostatic of narciclasine.—Van Goietsenoven, G., Hutton, J., Becker, J.-P., Lallemand, B., Robert, F., Lefranc, F., Pirker, C., Vandenbussche, G., Van Antwerpen, P., Evidente, A., Berger, W., Prévost, M., Pelletier, J., Kiss, R., Goss Kinzy, T., Kornienko, A., Mathieu, V. Targeting of eEF1A with Amaryllidaceae isocarbostyrils as a strategy to combat melanomas. PMID:20643906

  12. Bilirubin UDP-glucuronosyltransferase 1A1 gene polymorphisms: susceptibility to oxidative damage and cancer?

    PubMed

    Grant, D J; Bell, D A

    2000-12-01

    The UDP-glucuronosyltransferase 1A1 (UGT1A1) gene product catalyzes the glucuronidation of serum bilirubin as part of normal heme catabolism. Recently, TA repeat polymorphisms containing five, six, seven, and eight TA dinucleotides in a putative TATA box in the promoter region of the UGT1A1 gene have been described. TA repeat number modulates UGT1A1 transcriptional activity and the quantity of enzyme available to conjugate serum bilirubin. Serum bilirubin is a known antioxidant, and low serum bilirubin has been associated with increased risk for coronary artery disease and inhibition of reactive oxygen species (ROS)-induced damage to erythrocytes in vitro. We hypothesize that the UGT1A1 TA repeats or other functional polymorphisms resulting in lower serum bilirubin levels may be predictive of genetic susceptibility to oxidative damage and cancer. Exposure-related or endogenous production of ROS may impact the integrity of cellular macromolecules and infrastructure, lead to DNA base changes or chromosomal aberrations, and induce toxicity or apoptosis. ROS damage to lipoproteins may be a factor in formation of atherogenic plaques in coronary heart disease. Thus, cellular oxidative stress could contribute to tumorigenesis through mutagenic or epigenetic pathways, and higher serum bilirubin levels should inhibit this process. No definitive studies have been performed, but in a small prospective study of colon cancer, serum bilirubin levels were observed to be lower in these cases. Another study has suggested a link between UGT1A1 alleles, estrogen metabolism, and risk in breast cancer. Epidemiologic studies examining variation in ROS metabolism, ROS damage, bilirubin, and cancer risk will demonstrate the value of this hypothesis. PMID:11170257

  13. UGT1A1 genotype and irinotecan therapy: general review and implementation in routine practice.

    PubMed

    Etienne-Grimaldi, Marie-Christine; Boyer, Jean-Christophe; Thomas, Fabienne; Quaranta, Sylvie; Picard, Nicolas; Loriot, Marie-Anne; Narjoz, Céline; Poncet, Delphine; Gagnieu, Marie-Claude; Ged, Cécile; Broly, Franck; Le Morvan, Valérie; Bouquié, Régis; Gaub, Marie-Pierre; Philibert, Laurent; Ghiringhelli, François; Le Guellec, Chantal

    2015-06-01

    Irinotecan is a major drug in the treatment of advanced colorectal cancer. Its active form is the SN38 metabolite, which is cleared by the biliary route after glucuronidation by uridine diphosphate-glucuronosyltransferase 1A1 (UGT1A1). UGT1A1 activity exhibits a wide intersubject variability, in part related to UGT1A1 gene polymorphisms. The present review on the impact of the deficient UGT1A1*28 variant on irinotecan efficacy and toxicity was produced by a French joint workgroup comprising the Group of Clinical Onco-pharmacology (GPCO-Unicancer) and the National Pharmacogenetics Network (RNPGx). It clearly emerges that for irinotecan doses at least equal to 180 mg/m(2) , patients homozygous for the UGT1A1*28 allele are at increased risk of developing hematological and/or digestive toxicities. Irinotecan dose reduction is thus recommended in homozygous *28/*28 patients. In addition, this personalized medicine strategy aims to secure high-dose irinotecan administration (≥240 mg/m(2) ) that have proven to be safe in homozygous *1/*1 patients only. The clinical relevance of this test is discussed in terms of treatment efficacy improvement, as increasing the irinotecan dose appears to be safe in patients not bearing a deficient allele. Best execution practices, cost-effectiveness, and result interpretation are discussed with the aim of facilitating the implementation of this analysis in clinical practice. The existence of networks of laboratories performing this test in routine hospital treatment, as in France, offers the prospect of widespread screening, thus guaranteeing equal access to safe treatment and optimized therapy for patients receiving irinotecan-based therapy in advanced colorectal cancer. PMID:25817555

  14. Electroplated "cold patch" for critical parts

    NASA Technical Reports Server (NTRS)

    Tripp, H. A.

    1979-01-01

    Cracks in metal parts are filled by electrodeposition in plating tank that conforms to part to be repaired. Principle is applicable to repair of cracks in components of chemical reactors and other critical structures.

  15. Culturally and linguistically responsive teaching: part II.

    PubMed

    Billings, Diane M

    2015-03-01

    This part II of a two-part article about culturally and linguistically responsive teaching provides suggestions for evaluating learners who are culturally and linguistically diverse (CALD) and are often under-represented in nursing education settings.

  16. Voice Teachers on Voice, Part 3

    ERIC Educational Resources Information Center

    Gollobin, Laurie Brooks; White, Harvey

    1978-01-01

    Concludes a three-part symposium with eight prominent voice teachers on voice teaching methods. In this part, the teachers discuss placement, voice breaks, tone deafness, covered tone, and developing volume and offer some final general comments. (Editor)

  17. Talking about the Weather, Part II.

    ERIC Educational Resources Information Center

    Gibb, Allan A.

    1984-01-01

    This second part of a two-part article highlights some mathematics involved in the study of meteorology. Examples are given of the application of mathematics to the study of the atmosphere, with three problems discussed. (MNS)

  18. Intravital imaging of Bacillus thuringiensis Cry1A toxin binding sites in the midgut of silkworm.

    PubMed

    Li, Na; Wang, Jing; Han, Heyou; Huang, Liang; Shao, Feng; Li, Xuepu

    2014-02-15

    Identification of the resistance mechanism of insects against Bacillus thuringiensis Cry1A toxin is becoming an increasingly challenging task. This fact highlights the need for establishing new methods to further explore the molecular interactions of Cry1A toxin with insects and the receptor-binding region of Cry1A toxins for their wider application as biopesticides and a gene source for gene-modified crops. In this contribution, a quantum dot-based near-infrared fluorescence imaging method has been applied for direct dynamic tracking of the specific binding of Cry1A toxins, CrylAa and CrylAc, to the midgut tissue of silkworm. The in vitro fluorescence imaging displayed the higher binding specificity of CrylAa-QD probes compared to CrylAc-QD to the brush border membrane vesicles of midgut from silkworm. The in vivo imaging demonstrated that more CrylAa-QDs binding to silkworm midgut could be effectively and distinctly monitored in living silkworms. Furthermore, frozen section analysis clearly indicated the broader receptor-binding region of Cry1Aa compared to that of Cry1Ac in the midgut part. These observations suggest that the insecticidal activity of Cry toxins may depend on the receptor-binding sites, and this scatheless and visual near-infrared fluorescence imaging could provide a new avenue to study the resistance mechanism to maintain the insecticidal activity of B. thuringiensis toxins. PMID:24252542

  19. New Perspectives of Dyrk1A Role in Neurogenesis and Neuropathologic Features of Down Syndrome.

    PubMed

    Park, Joongkyu; Chung, Kwang Chul

    2013-12-01

    Down syndrome (DS) is one of the most common genetic disorders accompanying with mental retardation, cognitive impairment, and deficits in learning and memory. The brains with DS also display many neuropathological features including alteration in neurogenesis and synaptogenesis and early onset of Alzheimer's disease (AD)-like symptoms. Triplication of all or a part of human chromosome 21, especially the 21q22.1~21q22.3 region called 'Down syndrome critical region (DSCR)', has been considered as the main cause of DS. One gene product of DSCR, dual-specificity tyrosine-phosphorylation-regulated kinase 1A (Dyrk1A), has been highlighted as a key contributor to the neural consequences of DS. This minireview summarizes accumulating recent reports about Dyrk1A involvement in the neuritogenesis, synaptogenesis, and AD-like neurofibrillary tangle formation, which is mainly focusing on Dyrk1A-mediated regulation of cytoskeletal proteins, such as tubulin, actin, and microtubule-associated protein tau. Understanding the molecular mechanisms of these phenomena may provide us a rational for new preventive and therapeutic treatment of DS. PMID:24465139

  20. Triplication of DYRK1A causes retinal structural and functional alterations in Down syndrome.

    PubMed

    Laguna, Ariadna; Barallobre, María-José; Marchena, Miguel-Ángel; Mateus, Catarina; Ramírez, Erika; Martínez-Cue, Carmen; Delabar, Jean M; Castelo-Branco, Miguel; de la Villa, Pedro; Arbonés, Maria L

    2013-07-15

    Down syndrome (DS) results from the triplication of approximately 300 human chromosome 21 (Hsa21) genes and affects almost all body organs. Children with DS have defects in visual processing that may have a negative impact on their daily life and cognitive development. However, there is little known about the genes and pathogenesis underlying these defects. Here, we show morphometric in vivo data indicating that the neural retina is thicker in DS individuals than in the normal population. A similar thickening specifically affecting the inner part of the retina was also observed in a trisomic model of DS, the Ts65Dn mouse. Increased retinal size and cellularity in this model correlated with abnormal retinal function and resulted from an impaired caspase-9-mediated apoptosis during development. Moreover, we show that mice bearing only one additional copy of Dyrk1a have the same retinal phenotype as Ts65Dn mice and normalization of Dyrk1a gene copy number in Ts65Dn mice completely rescues both, morphological and functional phenotypes. Thus, triplication of Dyrk1a is necessary and sufficient to cause the retinal phenotype described in the trisomic model. Our data demonstrate for the first time the implication of DYRK1A overexpression in a developmental alteration of the central nervous system associated with DS, thereby providing insights into the aetiology of neurosensorial dysfunction in a complex disease. PMID:23512985

  1. BOREAS RSS-14 Level 1a GOES-7 Visible, IR, and Water Vapor Images

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Newcomer, Jeffrey A.; Faysash, David; Cooper, Harry J.; Smith, Eric A.

    2000-01-01

    The BOREAS RSS-14 team collected and processed GOES-7 and -8 images of the BOREAS region as part of its effort to characterize the incoming, reflected, and emitted radiation at regional scales. The level-1a BOREAS GOES-7 image data were collected by RSS-14 personnel at FSU and processed to level-1a products by BORIS personnel. The data cover the period of 01-Jan-1994 through 08-Jul-1995 with partial to complete coverage on the majority of the days. The data include three bands with eightbit pixel values. No major problems with the data have been identified. Due to the large size of the images, the level-1a GOES-7 data are not contained on the BOREAS CD-ROM set. An inventory listing file is supplied on the CD-ROM to inform users of what data were collected. The level-1a GOES-7 image data are available from the Earth Observing System Data and Information System (EOSDIS) Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC). See sections 15 and 16 for more information. The data files are available on a CD-ROM (see document number 20010000884).

  2. SOX2 suppresses CDKN1A to sustain growth of lung squamous cell carcinoma.

    PubMed

    Fukazawa, Takuya; Guo, Minzhe; Ishida, Naomasa; Yamatsuji, Tomoki; Takaoka, Munenori; Yokota, Etsuko; Haisa, Minoru; Miyake, Noriko; Ikeda, Tomoko; Okui, Tatsuo; Takigawa, Nagio; Maeda, Yutaka; Naomoto, Yoshio

    2016-01-01

    Since the SOX2 amplification was identified in lung squamous cell carcinoma (lung SCC), SOX2 transcriptional downstream targets have been actively investigated; however, such targets are often cell line specific. Here, in order to identify highly consensus SOX2 downstream genes in lung SCC cells, we used RNA-seq data from 178 lung SCC specimens (containing tumor and tumor-associated cells) and analyzed the correlation between SOX2 and previously-reported SOX2-controlled genes in lung SCC. In addition, we used another RNA-seq dataset from 105 non-small cell lung cancer cell lines (NSCLC; including 4 lung SCC cell lines) and again analyzed the correlation between SOX2 and the reported SOX2-controlled genes in the NSCLC cell lines (no tumor-associated cells). We combined the two analyses and identified genes commonly correlated with SOX2 in both datasets. Among the 99 genes reported as SOX2 downstream and/or correlated genes, we found 4 negatively-correlated (e.g., CDKN1A) and 11 positively-correlated genes with SOX2. We used biological studies to demonstrate that CDKN1A was suppressed by SOX2 in lung SCC cells. G1 cell cycle arrest induced by SOX2 siRNA was rescued by CDKN1A siRNA. These results indicate that the tumorigenic effect of SOX2 in lung SCC cells is mediated in part by suppression of CDKN1A. PMID:26846300

  3. 31 CFR 501.101 - Relation of this part to other parts in this chapter.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... in this chapter. 501.101 Section 501.101 Money and Finance: Treasury Regulations Relating to Money..., PROCEDURES AND PENALTIES REGULATIONS Relation of This Part to Other Parts in This Chapter § 501.101 Relation of this part to other parts in this chapter. This part sets forth standard reporting...

  4. Optical Stark spectroscopy of the B̃(1)A('')(000)←X̃(1)A(')(000) system of copper hydroxide.

    PubMed

    Wang, Fang; Steimle, Timothy C

    2011-01-01

    The B̃(1)A('')(000)←X̃(1)A(')(000) band system of a cold beam of CuOH has been studied field-free and in the presence of a static electric field. The Stark tuning of the low-J levels of the X̃(1)A(')(000) state were analyzed to give a value of 3.968(32) D for the a-component of the permanent electric dipole moment, μ(a). An upper limit of 0.3 D for μ(a)(B̃(1)A('')) is established from the lack of observable Stark tuning for the low-J levels of the B̃(1)A('')(000) state. The experimental value for μ(a)(X̃(1)A(')) is compared to theoretical predictions and other Cu-containing molecules. A molecular orbital correlation diagram is used to rationalize the large change in μ(a) upon excitation. The electronegativity of OH was determined to be 2.81 from a comparison of the determined μ(a) with the experimental μ values for CuF, CuO, and CuS.

  5. Optical Stark spectroscopy of the B~1A''(000)<--X~1A'(000) system of copper hydroxide

    NASA Astrophysics Data System (ADS)

    Wang, Fang; Steimle, Timothy C.

    2011-01-01

    The tilde B{}^1A^' ' (000)←tilde X{}^1A^' (000) band system of a cold beam of CuOH has been studied field-free and in the presence of a static electric field. The Stark tuning of the low-J levels of the tilde X{}^1A^' (000) state were analyzed to give a value of 3.968(32) D for the a-component of the permanent electric dipole moment, μa. An upper limit of 0.3 D for μa(tilde B{}^1A^' ' ) is established from the lack of observable Stark tuning for the low-J levels of the tilde B{}^1A^' ' (000) state. The experimental value for μa(tilde X{}^1A^' ) is compared to theoretical predictions and other Cu-containing molecules. A molecular orbital correlation diagram is used to rationalize the large change in μa upon excitation. The electronegativity of OH was determined to be 2.81 from a comparison of the determined μa with the experimental μ values for CuF, CuO, and CuS.

  6. The Alpha-1A Adrenergic Receptor in the Rabbit Heart.

    PubMed

    Thomas, R Croft; Cowley, Patrick M; Singh, Abhishek; Myagmar, Bat-Erdene; Swigart, Philip M; Baker, Anthony J; Simpson, Paul C

    2016-01-01

    The alpha-1A-adrenergic receptor (AR) subtype is associated with cardioprotective signaling in the mouse and human heart. The rabbit is useful for cardiac disease modeling, but data on the alpha-1A in the rabbit heart are limited. Our objective was to test for expression and function of the alpha-1A in rabbit heart. By quantitative real-time reverse transcription PCR (qPCR) on mRNA from ventricular myocardium of adult male New Zealand White rabbits, the alpha-1B was 99% of total alpha-1-AR mRNA, with <1% alpha-1A and alpha-1D, whereas alpha-1A mRNA was over 50% of total in brain and liver. Saturation radioligand binding identified ~4 fmol total alpha-1-ARs per mg myocardial protein, with 17% alpha-1A by competition with the selective antagonist 5-methylurapidil. The alpha-1D was not detected by competition with BMY-7378, indicating that 83% of alpha-1-ARs were alpha-1B. In isolated left ventricle and right ventricle, the selective alpha-1A agonist A61603 stimulated a negative inotropic effect, versus a positive inotropic effect with the nonselective alpha-1-agonist phenylephrine and the beta-agonist isoproterenol. Blood pressure assay in conscious rabbits using an indwelling aortic telemeter showed that A61603 by bolus intravenous dosing increased mean arterial pressure by 20 mm Hg at 0.14 μg/kg, 10-fold lower than norepinephrine, and chronic A61603 infusion by iPRECIO programmable micro Infusion pump did not increase BP at 22 μg/kg/d. A myocardial slice model useful in human myocardium and an anthracycline cardiotoxicity model useful in mouse were both problematic in rabbit. We conclude that alpha-1A mRNA is very low in rabbit heart, but the receptor is present by binding and mediates a negative inotropic response. Expression and function of the alpha-1A in rabbit heart differ from mouse and human, but the vasopressor response is similar to mouse. PMID:27258143

  7. The Alpha-1A Adrenergic Receptor in the Rabbit Heart

    PubMed Central

    Myagmar, Bat-Erdene; Swigart, Philip M.; Baker, Anthony J.; Simpson, Paul C.

    2016-01-01

    The alpha-1A-adrenergic receptor (AR) subtype is associated with cardioprotective signaling in the mouse and human heart. The rabbit is useful for cardiac disease modeling, but data on the alpha-1A in the rabbit heart are limited. Our objective was to test for expression and function of the alpha-1A in rabbit heart. By quantitative real-time reverse transcription PCR (qPCR) on mRNA from ventricular myocardium of adult male New Zealand White rabbits, the alpha-1B was 99% of total alpha-1-AR mRNA, with <1% alpha-1A and alpha-1D, whereas alpha-1A mRNA was over 50% of total in brain and liver. Saturation radioligand binding identified ~4 fmol total alpha-1-ARs per mg myocardial protein, with 17% alpha-1A by competition with the selective antagonist 5-methylurapidil. The alpha-1D was not detected by competition with BMY-7378, indicating that 83% of alpha-1-ARs were alpha-1B. In isolated left ventricle and right ventricle, the selective alpha-1A agonist A61603 stimulated a negative inotropic effect, versus a positive inotropic effect with the nonselective alpha-1-agonist phenylephrine and the beta-agonist isoproterenol. Blood pressure assay in conscious rabbits using an indwelling aortic telemeter showed that A61603 by bolus intravenous dosing increased mean arterial pressure by 20 mm Hg at 0.14 μg/kg, 10-fold lower than norepinephrine, and chronic A61603 infusion by iPRECIO programmable micro Infusion pump did not increase BP at 22 μg/kg/d. A myocardial slice model useful in human myocardium and an anthracycline cardiotoxicity model useful in mouse were both problematic in rabbit. We conclude that alpha-1A mRNA is very low in rabbit heart, but the receptor is present by binding and mediates a negative inotropic response. Expression and function of the alpha-1A in rabbit heart differ from mouse and human, but the vasopressor response is similar to mouse. PMID:27258143

  8. 40 CFR Table 1a to Subpart Ce of... - Emissions Limits for Small, Medium, and Large HMIWI at Designated Facilities as Defined in § 60...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... PERFORMANCE FOR NEW STATIONARY SOURCES Emission Guidelines and Compliance Times for Hospital/Medical/Infectious Waste Incinerators Pt. 60, Subpt. Ce, Table 1A Table 1A to Subpart Ce of Part 60—Emissions Limits... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Emissions Limits for Small, Medium,...

  9. 40 CFR Table 1a to Subpart Zzzz of... - Emission Limitations for Existing, New, and Reconstructed Spark Ignition, 4SRB Stationary RICE...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Internal Combustion Engines Pt. 63, Subpt. ZZZZ, Table 1a Table 1a to Subpart ZZZZ of Part 63—Emission... emissions by 75 percent or more until June 15, 2007 or Minimize the engine's time spent at idle and minimize the engine's startup time at startup to a period needed for appropriate and safe loading of the...

  10. 40 CFR Table 1a to Subpart Zzzz of... - Emission Limitations for Existing, New, and Reconstructed Spark Ignition, 4SRB Stationary RICE...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Internal Combustion Engines Pt. 63, Subpt. ZZZZ, Table 1a Table 1a to Subpart ZZZZ of Part 63—Emission..., 2007 or Minimize the engine's time spent at idle and minimize the engine's startup time at startup to a period needed for appropriate and safe loading of the engine, not to exceed 30 minutes, after which...

  11. 40 CFR Table 1a to Subpart Zzzz of... - Emission Limitations for Existing, New, and Reconstructed Spark Ignition, 4SRB Stationary RICE...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Internal Combustion Engines Pt. 63, Subpt. ZZZZ, Table 1a Table 1a to Subpart ZZZZ of Part 63—Emission... emissions by 75 percent or more until June 15, 2007 or Minimize the engine's time spent at idle and minimize the engine's startup time at startup to a period needed for appropriate and safe loading of the...

  12. 40 CFR Table 1a to Subpart Zzzz of... - Emission Limitations for Existing, New, and Reconstructed Spark Ignition, 4SRB Stationary RICE...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Internal Combustion Engines Pt. 63, Subpt. ZZZZ, Table 1a Table 1a to Subpart ZZZZ of Part 63—Emission... emissions by 75 percent or more until June 15, 2007 or Minimize the engine's time spent at idle and minimize the engine's startup time at startup to a period needed for appropriate and safe loading of the...

  13. 40 CFR Table 1a to Subpart Zzzz of... - Emission Limitations for Existing, New, and Reconstructed Spark Ignition, 4SRB Stationary RICE...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Internal Combustion Engines Pt. 63, Subpt. ZZZZ, Table 1a Table 1a to Subpart ZZZZ of Part 63—Emission... emissions by 75 percent or more until June 15, 2007 or Minimize the engine's time spent at idle and minimize the engine's startup time at startup to a period needed for appropriate and safe loading of the...

  14. Legislation and Part-Time Employment.

    ERIC Educational Resources Information Center

    Petersen, Allan L.; And Others

    This document presents an analysis of part-time employment issues in California community colleges, including a review of historical developments of the law covering the employment of certificated personnel, a review of some benefits and problems associated with part-time teaching, and consideration of part-time faculty employment as a major issue…

  15. Saturated Vapour Pressure and Refrigeration - Part I

    ERIC Educational Resources Information Center

    Bunker, C. A.

    1973-01-01

    The first part of a two-part article describes an experimental approach that can be used in teaching the concept of saturated vapour pressure. This leads to a discussion of refrigeration cycles in the second part of the article. (JR)

  16. Using GPU shaders for visualization, part 3.

    PubMed

    Bailey, Mike

    2013-01-01

    GPU shaders aren't just for glossy special effects. Parts 1 and 2 of this discussion looked at using them for point clouds, cutting planes, line integral convolution, and terrain bump-mapping. Part 3 covers compute shaders and shader storage buffer objects-two features announced as part of OpenGL 4.3.

  17. Part C Updates. 11th Edition

    ERIC Educational Resources Information Center

    Danaher, Joan; Goode, Sue; Lazara, Alex

    2010-01-01

    "Part C Updates" is a compilation of information on various aspects of the Early Intervention Program for Infants and Toddlers with Disabilities (Part C) of the Individuals with Disabilities Education Act (IDEA). This is the eleventh volume in a series of compilations, which included two editions of Part H Updates, the former name of the program.…

  18. Expertise revisited, Part I-Interactional expertise.

    PubMed

    Collins, Harry; Evans, Robert

    2015-12-01

    In Part I of this two part paper we try to set out the 'essence' of the notion of interactional expertise by starting with its origins. In Part II we will look at the notion of contributory expertise. The exercise has been triggered by recent discussion of these concepts in this journal by Plaisance and Kennedy and by Goddiksen.

  19. Part C Updates: 9th Edition

    ERIC Educational Resources Information Center

    Danaher, Joan; Goode, Sue; Lazara, Alex

    2007-01-01

    "Part C Updates" is a compilation of information on various aspects of the Early Intervention Program for Infants and Toddlers with Disabilities (Part C) of the Individuals with Disabilities Education Act (IDEA). This is the ninth volume in a series of compilations, which included two editions of Part H Updates, the former name of the program.…

  20. Parts, Cavities, and Object Representation in Infancy

    ERIC Educational Resources Information Center

    Hayden, Angela; Bhatt, Ramesh S.; Kangas, Ashley; Zieber, Nicole

    2011-01-01

    Part representation is not only critical to object perception but also plays a key role in a number of basic visual cognition functions, such as figure-ground segregation, allocation of attention, and memory for shapes. Yet, virtually nothing is known about the development of part representation. If parts are fundamental components of object shape…

  1. 7 CFR 1206.14 - Part.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE MANGO PROMOTION, RESEARCH, AND INFORMATION Mango Promotion, Research, and Information Order Definitions § 1206.14 Part. Part means part 1206 which includes the Mango Promotion, Research, and Information Order and all rules, regulations, and...

  2. 7 CFR 1206.14 - Part.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE MANGO PROMOTION, RESEARCH, AND INFORMATION Mango Promotion, Research, and Information Order Definitions § 1206.14 Part. Part means part 1206 which includes the Mango Promotion, Research, and Information Order and all rules, regulations, and...

  3. 7 CFR 1206.14 - Part.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE MANGO PROMOTION, RESEARCH, AND INFORMATION Mango Promotion, Research, and Information Order Definitions § 1206.14 Part. Part means part 1206 which includes the Mango Promotion, Research, and Information Order and all rules, regulations, and...

  4. 7 CFR 1206.14 - Part.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE MANGO PROMOTION, RESEARCH, AND INFORMATION Mango Promotion, Research, and Information Order Definitions § 1206.14 Part. Part means part 1206 which includes the Mango Promotion, Research, and Information Order and all rules, regulations, and...

  5. 7 CFR 1206.14 - Part.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE MANGO PROMOTION, RESEARCH, AND INFORMATION Mango Promotion, Research, and Information Order Definitions § 1206.14 Part. Part means part 1206 which includes the Mango Promotion, Research, and Information Order and all rules, regulations, and...

  6. SUMOylation of Syntaxin1A regulates presynaptic endocytosis.

    PubMed

    Craig, Tim J; Anderson, Dina; Evans, Ashley J; Girach, Fatima; Henley, Jeremy M

    2015-12-04

    Neurotransmitter release from the presynaptic terminal is under very precise spatial and temporal control. Following neurotransmitter release, synaptic vesicles are recycled by endocytosis and refilled with neurotransmitter. During the exocytosis event leading to release, SNARE proteins provide most of the mechanical force for membrane fusion. Here, we show one of these proteins, Syntaxin1A, is SUMOylated near its C-terminal transmembrane domain in an activity-dependent manner. Preventing SUMOylation of Syntaxin1A reduces its interaction with other SNARE proteins and disrupts the balance of synaptic vesicle endo/exocytosis, resulting in an increase in endocytosis. These results indicate that SUMOylation regulates the emerging role of Syntaxin1A in vesicle endocytosis, which in turn, modulates neurotransmitter release and synaptic function.

  7. Novel PRKAR1A gene mutations in Carney Complex.

    PubMed

    Pan, Lorraine; Peng, Lan; Jean-Gilles, J; Zhang, Ximin; Wieczorek, Rosemary; Jain, Shilpa; Levine, Vicki; Osman, Iman; Prieto, Victor G; Lee, Peng

    2010-01-01

    Carney complex is a syndrome that may include cardiac and mucocutaneous myxomas, spotting skin pigmentation, and endocrine lesions. Many patients with Carney complex have been shown to have a stop codon mutation in the PRKAR1A gene in the 17q22-24 region. Here we present the case of a 57 year-old man with multiple skin lesions and cardiac myxomas. Histology of the skin lesions showed lentigenous melanocytic hyperplasia and cutaneous myxomas, confirming the diagnosis of Carney complex. Lesional and control normal tissue from the patient were identified and sequenced for the PRKAR1A gene. A germline missense mutation was identified at exon 1A. This is the first report of this mutation, and one of the few reported missense mutation associated with Carney complex. This finding strengthens the argument that there are alternative ways in which the protein kinase A 1-alpha subunit plays a role in tumorigenesis. PMID:20606737

  8. Mutations in ALDH1A3 cause microphthalmia.

    PubMed

    Aldahmesh, M A; Khan, A O; Hijazi, H; Alkuraya, F S

    2013-08-01

    Microphthalmia is an important inborn error of eye development that can be associated with multisystem involvement. Anophthalmia is more severe and rarer. Single mutations in an expanding list of genes are known to cause this spectrum of anomaly. In one branch of a multiplex family with microphthalmia and anophthalmia, autozygome analysis excluded all known microphthalmia genes at the time of doing this study. Exome sequencing and autozygome filtration identified a novel homozygous variant in ALDH1A3. Subsequently, we identified another homozygous variant in 2 of the 10 probands with microphthalmia we specifically screened for mutations in ALDH1A3. Interestingly, the other branch of the original family was found to segregate anophthalmia/syndactyly with a novel homozygous SMOC1 variant. Our data support the very recent and independent identification of ALDH1A3 as a disease gene in microphthalmia. Locus heterogeneity should be considered in consanguineous families even for extremely rare phenotypes.

  9. Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts

    PubMed Central

    Morse, David; Webster, Wesley; Kalanon, Ming; Langsley, Gordon; McFadden, Geoffrey I.

    2016-01-01

    Over-expression of a GFP-PfRab1A fusion protein in Plasmodium falciparum schizonts produces a punctate pattern of fluorescence typical of rhoptries, secretory organelles involved in host cell invasion. The GFP-positive bodies were purified by a combination of differential and density gradient centrifugation and their protein content determined by MS/MS sequencing. Consistent with the GFP rhoptry-like pattern of transgenic parasites, four of the 19 proteins identified have been previously described to be rhoptry-associated and another four are ER or ER-associated proteins. Confirmation that GFP-PfRab1A decorates rhoptries was obtained by its co-localization with Rap1 and Ron4 in late phase schizonts. We conclude that PfRab1A potentially regulates vesicular traffic from the endoplasmic reticulum to the rhoptries in Apicomplexa parasites. PMID:27348424

  10. Acridone alkaloids from Glycosmis chlorosperma as DYRK1A inhibitors.

    PubMed

    Beniddir, Mehdi A; Le Borgne, Erell; Iorga, Bogdan I; Loaëc, Nadège; Lozach, Olivier; Meijer, Laurent; Awang, Khalijah; Litaudon, Marc

    2014-05-23

    Two new acridone alkaloids, chlorospermines A and B (1 and 2), were isolated from the stem bark of Glycosmis chlorosperma, together with the known atalaphyllidine (3) and acrifoline (4), by means of bioguided isolation using an in vitro enzyme assay against DYRK1A. Acrifoline (4) and to a lesser extent chlorospermine B (2) and atalaphyllidine (3) showed significant inhibiting activity on DYRK1A with IC50's of 0.075, 5.7, and 2.2 μM, respectively. Their selectivity profile was evaluated against a panel of various kinases, and molecular docking calculations provided structural details for the interaction between these compounds and DYRK1A. PMID:24798019

  11. Antenatal screening for HPA-1a by flow cytometry.

    PubMed

    Lavu, E K; Nelson, M; Popp, H J; Gibson, J; Kronenberg, H; Pearson, H; Child, A

    1997-05-01

    Pregnant women who attended antenatal clinics at King George V Hospital, the Birth Centre or were referred by obstetricians from February 19 July, 1996 were screened for the platelet antigen HPA-1a by flow cytometry. Forty out of 2,300 (1.7%) were found to be negative for this antigen. Of the 28 women followed throughout their pregnancy, none developed antibody to HPA-1a. Platelet counts performed on samples from 17 babies born to 17 of these mothers were all normal. This study proves the simplicity and rapidity of flow cytometry for platelet antigen screening. The results were comparable with the Solid Phase Red Cell Adherence (SPRCA) method and with PCR. The lack of a plentiful supply of specific antibody and the rarity of fetomaternal alloimmune thrombocytopenia (FMAIT) argue against the introduction of routine screening for maternal HPA-1a status at the present time.

  12. Postural instability in Charcot-Marie-Tooth 1A disease.

    PubMed

    Tozza, Stefano; Aceto, Maria Gabriella; Pisciotta, Chiara; Bruzzese, Dario; Iodice, Rosa; Santoro, Lucio; Manganelli, Fiore

    2016-09-01

    The aim of this study was to evaluate the influence of somatosensory impairment, distal muscle weakness and foot deformities on the balance in 21 CMT1A patients using a baropodometric platform. Stabilometric analysis by measuring sway area and velocity of a centre of pressure (CoP) both at open and closed eyes were used to assess postural imbalance. Static analysis, by measuring the load and the plantar surface of forefoot, midfoot and hindfoot was used to define the footprint shape and to assess as a whole foot deformities. Stabilometric and static results were compared with those of a control group. In CMT1A patients, stabilometric findings were correlated with static parameters, Achilles' tendon retraction, distal muscle strength and CMT examination score (CMTES). CMT1A patients compared to controls had lower plantar surface and load on midfoot, and higher load on a forefoot. CMT1A patients had a greater postural instability, since they had a higher CoP velocity, both at open and closed eyes. Moreover, the CoP velocity correlated inversely with the strength of ankle dorsi-flexion muscles and directly with CMTES as whole and with the item "motor symptoms legs". Postural imbalance was not correlated with sensory impairment and foot deformities as expressed by static analysis and Achilles' tendon retraction. In this study we demonstrated an altered balance in CMT1A patients during upright standing. The imbalance in our CMT patients seems to be related to the weakness of ankle dorsi-flexor muscles rather than sensory impairment or foot deformities. These results could be due to a mildly affected CMT1A population, evaluated in an early stage of the disease. PMID:27491052

  13. DYRK1A mutations in two unrelated patients.

    PubMed

    Ruaud, Lyse; Mignot, Cyril; Guët, Agnès; Ohl, Christelle; Nava, Caroline; Héron, Delphine; Keren, Boris; Depienne, Christel; Benoit, Valérie; Maystadt, Isabelle; Lederer, Damien; Amsallem, Daniel; Piard, Juliette

    2015-03-01

    The Dual-specify tyrosine phosphorylation-regulated kinase 1A (DYRK1A) gene has been extensively studied for its role in the pathophysiology of intellectual disability (ID) in Down syndrome. The rise of next generation sequencing (NGS) and array-CGH (aCGH) in diagnostic settings for the evaluation of patients with ID allowed the identification of 17 patients carrying heterozygous genetic aberrations involving DYRK1A to date. The rate of DYRK1A mutations in this population reaches >1% in published NGS studies. The current report aims at further defining the phenotype of this encephalopathy with the detailed report of two unrelated patients. Both patients were boys with developmental delay, febrile seizures, facial dysmorphism and brain atrophy on MRI. Patient #1 had autistic behaviors and micropenis and Patient #2 had stereotypies and microcephaly. NGS analyses identified heterozygous de novo variants in DYRK1A: the c.613C >T (p.Arg205*) nonsense mutation in Patient #1 and the c.932C >T (p.Ser311Phe) missense mutation in Patient #2. Together with previously reported cases, patients with DYRK1A mutations share many clinical features and may have a recognizable phenotype that includes, by decreasing order of frequency: developmental delay or ID with behaviors suggesting autism spectrum disorder, microcephaly, epileptic seizures, facial dysmorphism including ear anomalies (large ears, hypoplastic lobes), thin lips, short philtrum and frontal bossing. Delineation of the phenotype/genotype correlation is not feasible at the moment and will be a challenge for the coming years. PMID:25641759

  14. Mechanism of dual specificity kinase activity of DYRK1A.

    PubMed

    Walte, Agnes; Rüben, Katharina; Birner-Gruenberger, Ruth; Preisinger, Christian; Bamberg-Lemper, Simone; Hilz, Nikolaus; Bracher, Franz; Becker, Walter

    2013-09-01

    The function of many protein kinases is controlled by the phosphorylation of a critical tyrosine residue in the activation loop. Dual specificity tyrosine-phosphorylation-regulated kinases (DYRKs) autophosphorylate on this tyrosine residue but phosphorylate substrates on aliphatic amino acids. This study addresses the mechanism of dual specificity kinase activity in DYRK1A and related kinases. Tyrosine autophosphorylation of DYRK1A occurred rapidly during in vitro translation and did not depend on the non-catalytic domains or other proteins. Expression in bacteria as well as in mammalian cells revealed that tyrosine kinase activity of DYRK1A is not restricted to the co-translational autophosphorylation in the activation loop. Moreover, mature DYRK1A was still capable of tyrosine autophosphorylation. Point mutants of DYRK1A and DYRK2 lacking the activation loop tyrosine showed enhanced tyrosine kinase activity. A series of structurally diverse DYRK1A inhibitors was used to pharmacologically distinguish different conformational states of the catalytic domain that are hypothesized to account for the dual specificity kinase activity. All tested compounds inhibited substrate phosphorylation with higher potency than autophosphorylation but none of the tested inhibitors differentially inhibited threonine and tyrosine kinase activity. Finally, the related cyclin-dependent kinase-like kinases (CLKs), which lack the activation loop tyrosine, autophosphorylated on tyrosine both in vitro and in living cells. We propose a model of DYRK autoactivation in which tyrosine autophosphorylation in the activation loop stabilizes a conformation of the catalytic domain with enhanced serine/threonine kinase activity without disabling tyrosine phosphorylation. The mechanism of dual specificity kinase activity probably applies to related serine/threonine kinases that depend on tyrosine autophosphorylation for maturation. PMID:23809146

  15. 40 CFR Appendix A to Subpart Bb of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... (40 CFR Part 63, Subpart A) to Subpart BB A Appendix A to Subpart BB of Part 63 Protection of... to Subpart BB of Part 63—Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB 40 CFR citation Requirement Applies to subpart BB Comment 63.1(a)(1) through (4)...

  16. 40 CFR Appendix A to Subpart Bb of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (40 CFR Part 63, Subpart A) to Subpart BB A Appendix A to Subpart BB of Part 63 Protection of... to Subpart BB of Part 63—Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB 40 CFR citation Requirement Applies to subpart BB Comment 63.1(a)(1) through (4)...

  17. 40 CFR Appendix A to Subpart Bb of... - Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... (40 CFR Part 63, Subpart A) to Subpart BB A Appendix A to Subpart BB of Part 63 Protection of... to Subpart BB of Part 63—Applicability of General Provisions (40 CFR Part 63, Subpart A) to Subpart BB 40 CFR citation Requirement Applies to subpart BB Comment 63.1(a)(1) through (4)...

  18. Spatial orientation and dynamics of the U1A proteins in the U1A-UTR complex.

    PubMed

    Clerte, C; Hall, K B

    2000-06-20

    The human U1A protein contains three distinct domains: the N-terminal RBD1 (amino acids 1-101), the C-terminal RBD2 (amino acids 195-282), and the linker region (amino acids 102-194). The RBD1 domains of two U1A proteins bind specifically to two internal loops in the 3' untranslated region (3' UTR) of its own pre-mRNA. Tryptophan fluorescence and fluorescence resonance energy transfer data show that the two RBD2 domains do not interact with any regions of the UTR complex and display an overall tumbling that is uncorrelated from the core of the complex (formed by RBD1-UTR), indicating that the linker regions of the two U1A proteins remain flexible. The two RBD2 domains are separated by an apparent distance greater than 74 A in the UTR complex. The linker region adjacent to the RBD1 domain (103-ERDRKREKRKPKSQETP-119) is supposedly involved in protein-protein interactions (12). A single cysteine, introduced at position 101 or 121 of the U1A protein, was used as a specific attachment site for the fluorophore pair IAEDANS [N'-iodoacetyl-N'-(1-sulfo-5-n-naphthyl)ethylenediamine]/DABMI [4-(dimethylamino)-phenylazophenyl-4'-maleimide]. In the U1A-UTR complex (2:1), the dyes at the 101 position are separated by = approximately 51 A, while the dyes at the 121 position are at an apparent distance = approximately 58 A. The 101-121 crossed distance on adjacent U1A proteins averages to = 55 A. These results suggest that the amino acid sequence 101-121 of the two U1A proteins in the complex are held in proximity to each other in a compact conformation.

  19. Establishment of Salmonella strain expressing catalytically active human UDP-glucuronosyltransferase 1A1 (UGT1A1).

    PubMed

    Fujita, K; Mogami, A; Hayashi, A; Kamataki, T

    2000-04-01

    Human uridinediphosphate-glucuronosyltransferase 1A1 (UGT1A1) was expressed in Salmonella typhimurium TA1535 cells by transfection of the cells with plasmids carrying the UGT1A1 cDNA. UGT1A1 cDNA was isolated by a polymerase chain reaction from human liver total RNA and was inserted into the pSE420 plasmid, linked to the trc promoter and terminator. The plasmid thus constructed was introduced into Salmonella TA1535 cells. The expression of human UGT1A1 protein was confirmed by Western blot analysis. The maximal expression was observed at 24 h after the addition of isopropyl-beta-D-thiogalactopyranoside, an inducer. However, the bilirubin conjugation activity of the membrane fraction from the Salmonella cells was not detectable. When a beta-glucuronidase inhibitor such as saccharic acid 1,4-lactone, glycyrrhizin or 1-naphtyl-beta-D-glucuronide was added to the reaction mixture, the bilirubin conjugation activity of the human UGT1A1 was detected. When geniposide was added to the reaction mixture, the bilirubin conjugation activity of UGT1A1 was not seen. Taking these results into account, the established Salmonella strain possesses the beta-glucuronidase activity. Since the beta-glucuronidase activity of the Salmonella was lower than that of E. coli, it was concluded that Salmonella seemed to be a good host to express UGT protein. This is the first study to demonstrate the establishment of a bacterial strain expressing native human UGT protein showing catalytic activity. PMID:10821120

  20. D-1A nose fairing separation fitting load test

    NASA Technical Reports Server (NTRS)

    Vanvleet, J. O.

    1976-01-01

    Structural testing of the D-1A Centaur nose fairing was completed to determine the loads imposed during flight on the latch bolts of the fairing separation system. This testing was conducted to supplement and/or verify the analytic techniques used in calculating bolt loads for the D-1A, and to gain insight into the general structural behavior of separation latch systems. It was shown that the assumed bolt load magnification due to prying action of the latch fittings on the bolt does occur, but is strongly dependent on fairing shell stiffness.

  1. 40 CFR Table 1a to Subpart Ec of... - Emissions Limits for Small, Medium, and Large HMIWI at Affected Facilities as Defined in § 60.50c...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Table 1A to Subpart Ec of Part 60—Emissions Limits for Small, Medium, and Large HMIWI at Affected... 40 Protection of Environment 6 2010-07-01 2010-07-01 false Emissions Limits for Small, Medium, and Large HMIWI at Affected Facilities as Defined in § 60.50c(a)(1) and (2) 1A Table 1A to Subpart Ec...

  2. Asmparts: assembly of biological model parts.

    PubMed

    Rodrigo, Guillermo; Carrera, Javier; Jaramillo, Alfonso

    2007-12-01

    We propose a new computational tool to produce models of biological systems by assembling models from biological parts. Our software not only takes advantage of modularity, but it also enforces standardisation in part characterisation by considering a model of each part. We have used model parts in SBML to design transcriptional networks. Our software is open source, it works in linux and windows platforms, and it could be used to automatically produce models in a server. Our tool not only facilitates model design, but it will also help to promote the establishment of a registry of model parts.

  3. Asmparts: assembly of biological model parts

    PubMed Central

    Rodrigo, Guillermo; Carrera, Javier

    2008-01-01

    We propose a new computational tool to produce models of biological systems by assembling models from biological parts. Our software not only takes advantage of modularity, but it also enforces standardisation in part characterisation by considering a model of each part. We have used model parts in SBML to design transcriptional networks. Our software is open source, it works in linux and windows platforms, and it could be used to automatically produce models in a server. Our tool not only facilitates model design, but it will also help to promote the establishment of a registry of model parts. PMID:19003441

  4. Information for Inmates, Series # 1; A Bibliography of Fundamental Rights.

    ERIC Educational Resources Information Center

    Beale, Joel A., Comp.

    All of the books cited in this brief bibliography are annotated. Included are books on such subjects as: censorship, the moral and legal rights of prisoners, and basic human and civil rights. Some, if not all, of these books should be part of every prison library collection. (NH)

  5. Carnitine palmitoyl transferase-1A (CPT1A): a new tumor specific target in human breast cancer.

    PubMed

    Pucci, Sabina; Zonetti, Maria Josè; Fisco, Tommaso; Polidoro, Chiara; Bocchinfuso, Gianfranco; Palleschi, Antonio; Novelli, Giuseppe; Spagnoli, Luigi G; Mazzarelli, Paola

    2016-04-12

    Transcriptional mechanisms epigenetically-regulated in tumoral tissues point out new targets for anti-cancer therapies. Carnitine palmitoyl transferase I (CPT1) is the rate-limiting enzyme in the transport of long-chain fatty acids for β-oxidation. Here we identified the tumor specific nuclear CPT1A as a product of the transcript variant 2, that doesn't retain the classical transferase activity and is strongly involved in the epigenetic regulation of cancer pro-survival, cell death escaping and tumor invasion pathways. The knockdown of CPT1A variant 2 by small interfering RNAs (siRNAs), was sufficient to induce apoptosis in MCF-7, SK-BR3 and MDA-MB-231 breast cancer cells. The cell death triggered by CPT1A silencing correlated with reduction of HDAC activity and histone hyperacetylation. Docking experiments and molecular dynamics simulations confirmed an high binding affinity of the variant 2 for HDAC1. The CPT1A silenced cells showed an up-regulated transcription of pro-apoptotic genes (BAD, CASP9, COL18A1) and down-modulation of invasion and metastasis related-genes (TIMP-1, PDGF-A, SERPINB2). These findings provide evidence of the CPT1 variant 2 involvement in breast cancer survival, cell death escape and invasion. Thus, we propose nuclear CPT1A as a striking tumor specific target for anticancer therapeutics, more selective and effective as compared with the well-known HDAC inhibitors.

  6. Identification of Myb-binding protein 1A (MYBBP1A) as a novel substrate for aurora B kinase.

    PubMed

    Perrera, Claudia; Colombo, Riccardo; Valsasina, Barbara; Carpinelli, Patrizia; Troiani, Sonia; Modugno, Michele; Gianellini, Laura; Cappella, Paolo; Isacchi, Antonella; Moll, Jurgen; Rusconi, Luisa

    2010-04-16

    Aurora kinases are mitotic enzymes involved in centrosome maturation and separation, spindle assembly and stability, and chromosome condensation, segregation, and cytokinesis and represent well known targets for cancer therapy because their deregulation has been linked to tumorigenesis. The availability of suitable markers is of crucial importance to investigate the functions of Auroras and monitor kinase inhibition in in vivo models and in clinical trials. Extending the knowledge on Aurora substrates could help to better understand their biology and could be a source for clinical biomarkers. Using biochemical, mass spectrometric, and cellular approaches, we identified MYBBP1A as a novel Aurora B substrate and serine 1303 as the major phosphorylation site. MYBBP1A is phosphorylated in nocodazole-arrested cells and is dephosphorylated upon Aurora B silencing or by treatment with Danusertib, a small molecule inhibitor of Aurora kinases. Furthermore, we show that MYBBP1A depletion by RNA interference causes mitotic progression delay and spindle assembly defects. MYBBP1A has until now been described as a nucleolar protein, mainly involved in transcriptional regulation. The results presented herein show MYBBP1A as a novel Aurora B kinase substrate and reveal a not yet recognized link of this nucleolar protein to mitosis. PMID:20177074

  7. Structural determinants of odorant recognition by the human olfactory receptors OR1A1 and OR1A2.

    PubMed

    Schmiedeberg, Kristin; Shirokova, Elena; Weber, Hans-Peter; Schilling, Boris; Meyerhof, Wolfgang; Krautwurst, Dietmar

    2007-09-01

    An interaction of odorants with olfactory receptors is thought to be the initial step in odorant detection. However, ligands have been reported for only 6 out of 380 human olfactory receptors, with their structural determinants of odorant recognition just beginning to emerge. Guided by the notion that amino acid positions that interact with specific odorants would be conserved in orthologs, but variable in paralogs, and based on the prediction of a set of 22 of such amino acid positions, we have combined site-directed mutagenesis, rhodopsin-based homology modelling, and functional expression in HeLa/Olf cells of receptors OR1A1 and OR1A2. We found that (i) their odorant profiles are centred around citronellic terpenoid structures, (ii) two evolutionary conserved amino acid residues in transmembrane domain 3 are necessary for the responsiveness of OR1A1 and the mouse ortholog Olfr43 to (S)-(-)-citronellol, (iii) changes at these two positions are sufficient to account for the differential (S)-(-)-citronellol responsiveness of the paralogs OR1A1 and OR1A2, and (iv) the interaction sites for (S)-(-)-citronellal and (S)-(-)-citronellol differ in both human receptors. Our results show that the orientation of odorants within a homology modelling-derived binding pocket of olfactory receptor orthologs is defined by evolutionary conserved amino acid positions.

  8. Carnitine palmitoyl transferase-1A (CPT1A): a new tumor specific target in human breast cancer

    PubMed Central

    Zonetti, Maria Josè; Fisco, Tommaso; Polidoro, Chiara; Bocchinfuso, Gianfranco; Palleschi, Antonio; Novelli, Giuseppe; Spagnoli, Luigi G.

    2016-01-01

    Transcriptional mechanisms epigenetically-regulated in tumoral tissues point out new targets for anti-cancer therapies. Carnitine palmitoyl transferase I (CPT1) is the rate-limiting enzyme in the transport of long-chain fatty acids for β-oxidation. Here we identified the tumor specific nuclear CPT1A as a product of the transcript variant 2, that doesn't retain the classical transferase activity and is strongly involved in the epigenetic regulation of cancer pro-survival, cell death escaping and tumor invasion pathways. The knockdown of CPT1A variant 2 by small interfering RNAs (siRNAs), was sufficient to induce apoptosis in MCF-7, SK-BR3 and MDA-MB-231 breast cancer cells. The cell death triggered by CPT1A silencing correlated with reduction of HDAC activity and histone hyperacetylation. Docking experiments and molecular dynamics simulations confirmed an high binding affinity of the variant 2 for HDAC1. The CPT1A silenced cells showed an up-regulated transcription of pro-apoptotic genes (BAD, CASP9, COL18A1) and down-modulation of invasion and metastasis related-genes (TIMP-1, PDGF-A, SERPINB2). These findings provide evidence of the CPT1 variant 2 involvement in breast cancer survival, cell death escape and invasion. Thus, we propose nuclear CPT1A as a striking tumor specific target for anticancer therapeutics, more selective and effective as compared with the well-known HDAC inhibitors. PMID:26799588

  9. Identification of Myb-binding protein 1A (MYBBP1A) as a novel substrate for aurora B kinase.

    PubMed

    Perrera, Claudia; Colombo, Riccardo; Valsasina, Barbara; Carpinelli, Patrizia; Troiani, Sonia; Modugno, Michele; Gianellini, Laura; Cappella, Paolo; Isacchi, Antonella; Moll, Jurgen; Rusconi, Luisa

    2010-04-16

    Aurora kinases are mitotic enzymes involved in centrosome maturation and separation, spindle assembly and stability, and chromosome condensation, segregation, and cytokinesis and represent well known targets for cancer therapy because their deregulation has been linked to tumorigenesis. The availability of suitable markers is of crucial importance to investigate the functions of Auroras and monitor kinase inhibition in in vivo models and in clinical trials. Extending the knowledge on Aurora substrates could help to better understand their biology and could be a source for clinical biomarkers. Using biochemical, mass spectrometric, and cellular approaches, we identified MYBBP1A as a novel Aurora B substrate and serine 1303 as the major phosphorylation site. MYBBP1A is phosphorylated in nocodazole-arrested cells and is dephosphorylated upon Aurora B silencing or by treatment with Danusertib, a small molecule inhibitor of Aurora kinases. Furthermore, we show that MYBBP1A depletion by RNA interference causes mitotic progression delay and spindle assembly defects. MYBBP1A has until now been described as a nucleolar protein, mainly involved in transcriptional regulation. The results presented herein show MYBBP1A as a novel Aurora B kinase substrate and reveal a not yet recognized link of this nucleolar protein to mitosis.

  10. 7. 'FLOW IN CANAL NO. 1, A JOINTLY USED CANAL, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. 'FLOW IN CANAL NO. 1, A JOINTLY USED CANAL, ON MAY 22 WHEN 210 SECOND FEET OF WATER WAS FLOWING. THIS WAS LATER INCREASED TO 240 SECOND FEET FOR A NUMBER OF DAYS TO SATISFY THE DEMANDS OF THE DRY GULCH COMPANY.' 1925 - Irrigation Canals in the Uinta Basin, Duchesne, Duchesne County, UT

  11. Calcium kinetics in glycogen storage disease type 1a.

    PubMed

    Goans, R E; Weiss, G H; Vieira, N E; Sidbury, J B; Abrams, S A; Yergey, A L

    1996-12-01

    Glycogen storage disease type 1a (Von Gierke's disease) is one of the more common glycogen storage diseases (GSD). GSD 1a patients can have severe idiopathic osteopenia, often beginning at a young age. Since calcium tracer studies offer a sensitive probe of the bone microenvironment and of calcium deposition, kinetics might be disturbed in patients with GSD 1a. Plasma dilution kinetics obtained using the stable isotope 42Ca are shown in this paper to be quite different between GSD 1a patients and age-matched controls. Comparison of kinetic parameters in these two populations is made using a new binding site model for describing calcium dynamics at the plasma-bone interface. This model describes reversible binding of calcium ions to postulated short-term and long-term sites by a retention probability density function psi (t). Using this analysis, adult GSD subjects exhibited a significant decrease (P = 0.023) in the apparent half-life of a calcium ion on the longer-term site compared with controls. The general theory of calcium tracer dilution kinetics is then discussed in terms of a new model of short-term calcium homeostasis recently proposed by Bronner and Stein [5]. PMID:8939770

  12. Passive smoking, Cyp1A1 gene polymorphism and dysmenorrhea

    PubMed Central

    Liu, Hong; Yang, Fan; Li, Zhiping; Chen, Changzhong; Fang, Zhian; Wang, Lihua; Hu, Yonghua; Chen, Dafang

    2007-01-01

    Objective This study investigated whether the association between passive smoking exposure and dysmenorrhea is modified by two susceptibility genes, CYP1A1MspI and CYP1A1HincII. Methods This report includes 1645 (1124 no dysmenorrhea, 521 dysmenorrhea) nonsmoking and nondrinking newly wed female workers at Anqing, China between June 1997 and June 2000. Multiple logistic regression models were used to estimate the associations of passive smoking exposure and genetic susceptibility with dysmenorrhea, adjusting for perceived stress. Results When stratified by women genotype, the adjusted OR of dysmenorrhea was 1.6 (95%CI=1.3-2.1) for passive smoking group with Ile/Ile462 genotype, and 1.5 (95%CI=1.1-2.1) with C/C6235 genotype, compared to non passive smoking group, respectively. The data further showed that there was a significant combined effect between passive smoking and the CYP1A1 Msp1 C/C6235 and HincII Ile/Ile462 genotype (OR=2.6, 95%CI=1.3-5.2). Conclusion CYP1A1 MspI and HincII genotypes modified the association between passive smoking and dysmenorrhea. PMID:17566695

  13. 26 CFR 1.669(a)-1A - Amount allocated.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...)-1A Internal Revenue INTERNAL REVENUE SERVICE, DEPARTMENT OF THE TREASURY (CONTINUED) INCOME TAX (CONTINUED) INCOME TAXES Treatment of Excess Distributions of Trusts Applicable to Taxable Years Beginning... of its undistributed net income, the rules concerning the treatment of capital gain...

  14. Complete Genome Sequence of Elephant Endotheliotropic Herpesvirus 1A.

    PubMed

    Ling, Paul D; Reid, Jeffrey G; Qin, Xiang; Muzny, Donna M; Gibbs, Richard; Petrosino, Joseph; Peng, Rongsheng; Zong, Jian-Chao; Heaggans, Sarah Y; Hayward, Gary S

    2013-01-01

    Elephant endotheliotropic herpesvirus 1A is a member of the Proboscivirus genus and is a major cause of fatal hemorrhagic disease in endangered juvenile Asian elephants worldwide. Here, we report the first complete genome sequence from this genus, obtained directly from necropsy DNA, in which 60 of the 115 predicted genes are not found in any known herpesvirus.

  15. Mutations in pseudohypoparathyroidism 1a and pseudopseudohypoparathyroidism in ethnic Chinese.

    PubMed

    Wu, Yi-Lei; Hwang, Daw-Yang; Hsiao, Hui-Pin; Ting, Wei-Hsin; Huang, Chi-Yu; Tsai, Wen-Yu; Chen, Hung-Chun; Chao, Mei-Chyn; Lo, Fu-Sung; Tsai, Jeng-Daw; Yang, Stone; Shih, Shin-Lin; Lin, Shuan-Pei; Lin, Chiung-Ling; Lee, Yann-Jinn

    2014-01-01

    An inactivating mutation in the GNAS gene causes either pseudohypoparathyroidism 1a (PHP1A) when it is maternally inherited or pseudopseudohypoparathyroidism (PPHP) when it is paternally inherited. We investigated clinical manifestations and mutations of the GNAS gene in ethnic Chinese patients with PHP1A or PPHP. Seven patients from 5 families including 4 girls and 2 boys with PHP1A and 1 girl with PPHP were studied. All PHP1A patients had mental retardation. They were treated with calcitriol and CaCO3 with regular monitoring of serum Ca levels, urinary Ca/Cr ratios, and renal sonography. Among them, 5 patients also had primary hypothyroidism suggesting TSH resistance. One female patient had a renal stone which was treated with extracorporeal shockwave lithotripsy. She had an increased urinary Ca/Cr ratio of 0.481 mg/mg when the stone was detected. We detected mutations using PCR and sequencing as well as analysed a splice acceptor site mutation using RT-PCR, sequencing, and minigene construct. We detected 5 mutations: c.85C>T (Q29*), c.103C>T (Q35*), c.840-2A>G (R280Sfs*21), c.1027_1028delGA (D343*), and c.1174G>A (E392K). Mutations c.840-2A>G and c.1027_1028delGA were novel. The c.840-2A>G mutation at the splice acceptor site of intron 10 caused retention of intron 10 in the minigene construct but skipping of exon 11 in the peripheral blood cells. The latter was the most probable mechanism which caused a frameshift, changing Arg to Ser at residue 280 and invoking a premature termination of translation at codon 300 (R280Sfs*21). Five GNAS mutations in ethnic Chinese with PHP1A and PPHP were reported. Two of them were novel. Mutation c.840-2A>G destroyed a spice acceptor site and caused exon skipping. Regular monitoring and adjustment in therapy are mandatory to achieve optimal therapeutic effects and avoid nephrolithiasis in patients with PHP1A.

  16. Parkinson's disease and CYP1A2 activity

    PubMed Central

    Forsyth, J T; Grünewald, R A; Rostami-Hodjegan, A; Lennard, M S; Sagar, H J; Tucker, G T

    2000-01-01

    Aims MPTP, a neurotoxin which induces parkinsonism is partially metabolized by the enzyme CYP1A2. Smoking appears to protect against Parkinson's disease (PD) and cigarette smoke induces CYP1A2 activity. Thus, we investigated the hypothesis that idiopathic PD is associated with lower CYP1A2 activity using caffeine as a probe compound. Methods CYP1A2 activity was assessed using saliva paraxanthine (PX) to caffeine (CA) ratios. Caffeine half-life was also estimated from salivary concentrations of caffeine at 2 and 5 h post dose. 117 treated and 40 untreated patients with PD and 105 healthy control subjects were studied. Results PX/CA ratios were 0.57, 0.93 and 0.77 in treated patients, untreated patients and healthy control subjects, respectively, with no significant differences between study groups (95% CI: treated patients vs controls −0.24, 0.57; untreated patients vs controls −0.75, 0.35). However, patients with PD (treated or untreated) had caffeine half-lives shorter than that in controls (treated patients: 262 min, untreated patients: 244 min, controls: 345 min; 95% CI: controls vs treated patients 23, 143 (P = 0.003); controls vs untreated patients 19, 184 (P = 0.011)). Amongst the patients with PD, caffeine half-life was also inversely related to the age of onset of disease (P = 0.012); gender and concomitant drugs did not influence this significantly. Conclusions Based on PX/CA ratio, there was no evidence of decreased CYP1A2 activity in patients compared with control subjects. The observed decrease in the elimination half-life of caffeine in PD may be caused by increased CYP2E1 activity, an enzyme that also contributes to the metabolism of caffeine. The latter warrants further investigation. PMID:11012552

  17. Subregions of the adenovirus E1A transactivation domain target multiple components of the TFIID complex.

    PubMed Central

    Geisberg, J V; Chen, J L; Ricciardi, R P

    1995-01-01

    Transcriptional activation by the adenovirus E1A 289R protein requires direct contacts with the TATA box-binding protein (TBP) and also displays a critical requirement for TBP-associated factors (TAFs) (T.G. Boyer and A. J. Berk, Genes Dev. 7:1810-1823, 1993; J. V. Geisberg, W. S. Lee, A. J. Berk, and R. P. Ricciardi, Proc. Natl. Acad. Sci. USA 91:2488-2492, 1994; W. S. Lee, C. C. Kao, G. O. Bryant, X. Liu, and A. J. Berk, Cell 67:365-376, 1991; and Q. Zhou, P. M. Lieberman, T. G. Boyer, and A. J. Berk, Genes Dev. 6:1964-1974, 1992). In this report, we demonstrate that the activation domain of E1A (CR3) specifically binds to two TAFs, human TAFII250 (hTAFII250) and Drosophila TAFII110 (dTAFII110). These interactions can take place both in vivo and in vitro and require the carboxy-terminal region of CR3; the zinc finger region of CR3, which binds TBP, is not needed to bind these TAFs. We mapped the E1A-binding sites on hTAFII250 to an internal region that contains a number of structural motifs, including an HMG box, a bromodomain, and direct repeats. This represents the first demonstration that hTAFII250 may serve as a target of a transcriptional activator. We also mapped the E1A binding on dTAFII110 to its C-terminal region. This is of significance since, by contrast, Sp1-mediated activation requires binding to the N-terminal domain of dTAFII110. Thus, distinct surfaces of dTAFII110 can serve as target sites for different activators. Our results indicate that E1A may activate transcription, in part, through direct contacts of the CR3 subdomains with selected components of the TFIID complex. PMID:7565781

  18. Using partial sensor information to orient parts

    SciTech Connect

    Akella, S.; Mason, M.T.

    1999-10-01

    Parts orienting, the process of bringing parts in initially unknown orientations to a goal orientation, is an important aspect of automated assembly. The most common industrial orienting systems are vibratory bowl feeders, which use the shape and mass properties of parts to orient them. Bowl feeders rely on a sequence of mechanical operations and typically do not use sensors. In this paper, the authors describe the use of partial information sensors along with a sequence of pushing operations to eliminate uncertainty in the orientation of parts. The authors characterize the shorter execution lengths of sensor-based plans and show that sensor-based plans are more powerful than sensorless plans in that they can bring a larger class of parts to distinct orientations. The authors characterize the relation among part shape, orientability; and recognizability to identify conditions under which a single plan can orient and recognize multiple part shapes. Although part shape determines the results of the actions and the sensed information, the authors establish that differences in part shape do not always lead to differences in part behavior. The authors show that for any convex polygon, there exists an infinite set of nonsimilar convex polygons that behave identically under linear normal pushes. Furthermore, there exists a infinite set of nonsimilar convex polygons whose behavior cannot be distinguished even with diameter sensing after each push. They authors have implemented several planners and demonstrated generated plans in experiments.

  19. Evidence for the effect of serotonin receptor 1A gene (HTR1A) polymorphism on tractability in Thoroughbred horses.

    PubMed

    Hori, Y; Tozaki, T; Nambo, Y; Sato, F; Ishimaru, M; Inoue-Murayama, M; Fujita, K

    2016-02-01

    Tractability, or how easily animals can be trained and controlled, is an important behavioural trait for the management and training of domestic animals, but its genetic basis remains unclear. Polymorphisms in the serotonin receptor 1A gene (HTR1A) have been associated with individual variability in anxiety-related traits in several species. In this study, we examined the association between HTR1A polymorphisms and tractability in Thoroughbred horses. We assessed the tractability of 167 one-year-old horses reared at a training centre for racehorses using a questionnaire consisting of 17 items. A principal components analysis of answers contracted the data to five principal component (PC) scores. We genotyped two non-synonymous single nucleotide polymorphisms (SNPs) in the horse HTR1A coding region. We found that one of the two SNPs, c.709G>A, which causes an amino acid change at the intracellular region of the receptor, was significantly associated with scores of four of five PCs in fillies (all Ps < 0.05) and one PC in colts (P < 0.01). Horses carrying an A allele at c.709G>A showed lower tractability. This result provides the first evidence that a polymorphism in a serotonin-related gene may affect tractability in horses with the effect partially different depending on sex. PMID:26763159

  20. Evidence for the effect of serotonin receptor 1A gene (HTR1A) polymorphism on tractability in Thoroughbred horses.

    PubMed

    Hori, Y; Tozaki, T; Nambo, Y; Sato, F; Ishimaru, M; Inoue-Murayama, M; Fujita, K

    2016-02-01

    Tractability, or how easily animals can be trained and controlled, is an important behavioural trait for the management and training of domestic animals, but its genetic basis remains unclear. Polymorphisms in the serotonin receptor 1A gene (HTR1A) have been associated with individual variability in anxiety-related traits in several species. In this study, we examined the association between HTR1A polymorphisms and tractability in Thoroughbred horses. We assessed the tractability of 167 one-year-old horses reared at a training centre for racehorses using a questionnaire consisting of 17 items. A principal components analysis of answers contracted the data to five principal component (PC) scores. We genotyped two non-synonymous single nucleotide polymorphisms (SNPs) in the horse HTR1A coding region. We found that one of the two SNPs, c.709G>A, which causes an amino acid change at the intracellular region of the receptor, was significantly associated with scores of four of five PCs in fillies (all Ps < 0.05) and one PC in colts (P < 0.01). Horses carrying an A allele at c.709G>A showed lower tractability. This result provides the first evidence that a polymorphism in a serotonin-related gene may affect tractability in horses with the effect partially different depending on sex.

  1. Quality Assurance and Quality Control, Part 2.

    PubMed

    Akers, Michael J

    2015-01-01

    The tragedy surrounding the New England Compounding Center and contaminated steroid syringe preparations clearly points out what can happen if quality-assurance and quality-control procedures are not strictly practiced in the compounding of sterile preparations. This article is part 2 of a two-part article on requirements to comply with United States Pharmacopeia general chapters <797> and <1163> with respect to quality assurance of compounded sterile preparations. Part 1 covered documentation requirements, inspection procedures, compounding accuracy checks, and part of a discussion on bacterial endotoxin testing. Part 2 covers sterility testing, the completion from part 1 on bacterial endotoxin testing, a brief dicussion of United States Pharmacopeia <1163>, and advances in pharmaceutical quality systems.

  2. Amylase α-1A (AMY1A): a novel immunohistochemical marker to differentiate chromophobe renal cell carcinoma from benign oncocytoma.

    PubMed

    Jain, Sarika; Roy, Somak; Amin, Milon; Acquafondata, Marie; Yin, Ming; Laframboise, William; Bastacky, Sheldon; Pantanowitz, Liron; Dhir, Rajiv; Parwani, Anil

    2013-12-01

    Chromophobe renal cell carcinoma (ChRCC) and oncocytoma present with a perplexing overlap of morphologic and immunohistochemical features. ChRCC have deletions in the 1p21.1 region including the amylase α-1A gene (AMY1A). No such deletions are found in oncocytoma. Instead, oncocytomas shared other deletions on chromosome 1: 1p31.3, 1q25.2, and 1q44. We performed AMY1A immunostaining on 75 oncocytomas (57 tissue microarray [TMA] cores, 18 whole slides) and 54 ChRCCs (20 TMA cores, 34 whole slides). Staining was assessed using the H-score method. The intensity was graded as follows: no staining=0, weak=1, moderate=2, and strong=3. The AMY1A immunostain preferentially stained the distal tubules and collecting ducts of normal kidney. All oncocytomas (100%) expressed AMY1A with an H-score that varied from 100 to 300 (mean 205). Mild to moderate heterogeneity in staining intensity was noted within a given oncocytoma. For oncocytomas, 87% (65/75) cases had H-scores of at least 120 with a mean score of 221. Notably, the 13% (10/75) of oncocytoma cases that had an H-score of 100 were derived from the TMA. A total of 87% (47/54) of the ChRCC cases were negative for the AMY1A immunostain. Of the ChRCC cases, 4% (2/54) showed very weak cytoplasmic staining (H-score of 70 each), which was less than the lowest H-score of oncocytoma cases. All 5 cases of ChRCC, which showed an H-score of 100 or more, were referred to as eosinophilic variants of ChRCC. Three of these 5 cases showed a very nondescript, diffuse staining of the cytoplasm. Two of these 5 cases showed an H-score of 130. We think that as the staining pattern of these 2 cases is similar to that of oncocytoma, they should be put in a category of renal oncocytic neoplasms favoring oncocytoma. This result shows that AMY1A staining could be very helpful in further classifying even a subset of the eosinophilic variants of ChRCC. The difference between ChRCC and oncocytoma was statistically significant (χ test, P<0

  3. Cooperative Force Generation of KIF1A Brownian Motors

    NASA Astrophysics Data System (ADS)

    Oriola, David; Casademunt, Jaume

    2013-07-01

    KIF1A is a kinesin motor protein that can work processively in a monomeric (single-headed) form by using a noise-driven ratchet mechanism. Here, we show that the combination of a passive diffusive state and finite-time kinetics of adenosine triphosphate hydrolysis provides a powerful mechanism of cooperative force generation, implying for instance that ˜10 monomeric KIF1As can team up to become ˜100 times stronger than a single one. Consequently, we propose that KIF1A could outperform conventional (double-headed) kinesin collectively and thus explain its specificity in axonal trafficking. We elucidate the cooperativity mechanism with a lattice model that includes multiparticle transitions.

  4. 45 CFR Appendix A to Part 1110 - Federal Financial Assistance to Which This Part Applies

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Federal Financial Assistance to Which This Part Applies A Appendix A to Part 1110 Public Welfare Regulations Relating to Public Welfare (Continued... Pt. 1110, App. A Appendix A to Part 1110—Federal Financial Assistance to Which This Part Applies...

  5. 33 CFR Appendix A to Part 385 - Illustrations to Part 385

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Illustrations to Part 385 A Appendix A to Part 385 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY.... A Appendix A to Part 385—Illustrations to Part 385 ER12NO03.000 ER12NO03.001...

  6. 49 CFR Appendix A-Ii to Part 541 - Lines With Antitheft Devices Which Are Exempted in-Part From the Parts-Marking Requirements of...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...-Part From the Parts-Marking Requirements of This Standard Pursuant to 49 CFR Part 543 A Appendix A-II... STANDARD Pt. 541, App. A-II Appendix A-II to Part 541—Lines With Antitheft Devices Which Are Exempted in-Part From the Parts-Marking Requirements of This Standard Pursuant to 49 CFR Part 543...

  7. Machining Thin-Walled Cylindrical Parts

    NASA Technical Reports Server (NTRS)

    Cimbak, Joe; Spagnolo, Jim; Kraus, Dan

    1988-01-01

    Cylindrical walls only few thousandths of inch thick machined accurately and without tears or punctures with aid of beryllium copper mandrel. Chilled so it contracts, then inserted in cylinder. As comes to room temperature, mandrel expands and fits snugly inside cylinder. Will not allow part to slide and provides solid backup to prevent deflection when part machined by grinding wheel. When machining finished, cylinder-and-mandrel assembly inserted in dry ice, mandrel contracts and removed from part.

  8. Parts Engineering Experiences, Philosophies and Trends

    NASA Technical Reports Server (NTRS)

    Shaw, Harry; Day, John H. (Technical Monitor)

    2000-01-01

    This document is the presentation viewgraphs of the NASA presentations to NASDA, outlining the philosophy and trends of the experiences with engineering parts. Included in the presentations: are (1) the assurance of COTS boards for Space flight, and (2) Peer Review for Ice, Cloud and land Elevation Satellite (ICESat) GPS flight receivers EEE parts. The emphasis is on the methods for qualification of available parts for space flight.

  9. Basic Principles of Lyophilization, Part 1.

    PubMed

    Akers, Michael J

    2015-01-01

    The achievement of a high-quality lyophilized (freeze-dried) dosage form involves the combination of optimal formulation design and optimal freeze-dry cycle design. This 2-part article describes how this can be done. Part 1 discusses the basic principles and procedures of lyophilization up to a discussion on the different stages of lyophilization. The stages of lyophilization are discussed in part 2.

  10. Total synthesis of avermectin B1a revisited.

    PubMed

    Yamashita, Shuji; Hayashi, Daisuke; Nakano, Aoi; Hayashi, Yujiro; Hirama, Masahiro

    2016-01-01

    Avermectins were isolated as compounds possessing anthelmintic activity from the culture broth of Streptomycesavermitilis by Ōmura and co-workers. Owing to their potent anthelmintic and insecticidal activities, as well as their unique pentacyclic architecture, the avermectin family attracted keen interest from synthetic organic chemists. We have recently completed a more efficient and straightforward total synthesis of avermectin B1a, as compared with previous syntheses. PMID:26350782

  11. ALDH1A3 mutations cause recessive anophthalmia and microphthalmia.

    PubMed

    Fares-Taie, Lucas; Gerber, Sylvie; Chassaing, Nicolas; Clayton-Smith, Jill; Hanein, Sylvain; Silva, Eduardo; Serey, Margaux; Serre, Valérie; Gérard, Xavier; Baumann, Clarisse; Plessis, Ghislaine; Demeer, Bénédicte; Brétillon, Lionel; Bole, Christine; Nitschke, Patrick; Munnich, Arnold; Lyonnet, Stanislas; Calvas, Patrick; Kaplan, Josseline; Ragge, Nicola; Rozet, Jean-Michel

    2013-02-01

    Anophthalmia and microphthalmia (A/M) are early-eye-development anomalies resulting in absent or small ocular globes, respectively. A/M anomalies occur in syndromic or nonsyndromic forms. They are genetically heterogeneous, some mutations in some genes being responsible for both anophthalmia and microphthalmia. Using a combination of homozygosity mapping, exome sequencing, and Sanger sequencing, we identified homozygosity for one splice-site and two missense mutations in the gene encoding the A3 isoform of the aldehyde dehydrogenase 1 (ALDH1A3) in three consanguineous families segregating A/M with occasional orbital cystic, neurological, and cardiac anomalies. ALDH1A3 is a key enzyme in the formation of a retinoic acid gradient along the dorso-ventral axis during early eye development. Transitory expression of mutant ALDH1A3 open reading frames showed that both missense mutations reduce the accumulation of the enzyme, potentially leading to altered retinoic acid synthesis. Although the role of retinoic acid signaling in eye development is well established, our findings provide genetic evidence of a direct link between retinoic-acid-synthesis dysfunction and early-eye-development anomalies in humans.

  12. Incomplete penetrance of biallelic ALDH1A3 mutations.

    PubMed

    Plaisancié, Julie; Brémond-Gignac, Dominique; Demeer, Bénédicte; Gaston, Véronique; Verloes, Alain; Fares-Taie, Lucas; Gerber, Sylvie; Rozet, Jean-Michel; Calvas, Patrick; Chassaing, Nicolas

    2016-04-01

    The formation of a properly shaped eye is a complex developmental event that requires the coordination of many induction processes and differentiation pathways. Microphthalmia and anophthalmia (MA) represent the most severe defects that can affect the ocular globe during embryonic development. When genetic, these ocular disorders exhibit large genetic heterogeneity and extreme variable expressivity. Around 20 monogenic diseases are known to be associated with MA as main phenotype and the penetrance of mutations is usually full in the patients. Some of these genes encode proteins involved in the vitamin A pathway, tightly regulated during eye development. One of those retinoic acid synthesis genes is ALDH1A3 and biallelic mutations in that gene have been recently found to lead to MA phenotype in patients. Interestingly, we report here the lack of ocular defect in a girl carrying the same homozygous mutation in the ALDH1A3 gene than the affected members of her family. Thus, this report brings new information for the phenotype-genotype correlation of ALDH1A3 mutations and raises important questions, especially in terms of genetic counselling given to the patients and their families. Furthermore, these data contribute to the more general understanding that we have for the complex genetic inheritance of these MA phenotypes. PMID:26873617

  13. Aberrant expression of RAB1A in human tongue cancer

    PubMed Central

    Shimada, K; Uzawa, K; Kato, M; Endo, Y; Shiiba, M; Bukawa, H; Yokoe, H; Seki, N; Tanzawa, H

    2005-01-01

    This study was designed to identify specific gene expression changes in tongue squamous cell carcinomas (TSCCs) compared with normal tissues using in-house cDNA microarray that comprised of 2304 full-length cDNAs from a cDNA library prepared from normal oral tissues, primary oral cancers, and oral cancer cell lines. The genes identified by our microarray system were further analysed at the mRNA or protein expression level in a series of clinical samples by real-time quantitative reverse transcriptase–polymerase chain reaction (qRT–PCR) analysis and imuunohositochemistry. The microarray analysis identified a total of 16 genes that were significantly upregulated in common among four TSCC specimens. Consistent with the results of the microarray, increased mRNA levels of selected genes with known molecular functions were found in the four TSCCs. Among genes identified, Rab1a, a member of the Ras oncogene family, was further analysed for its protein expression in 54 TSCCs and 13 premalignant lesions. We found a high prevalence of Rab1A-overexpression not only in TSCCs (98%) but also in premalignant lesions (93%). Thus, our results suggest that rapid characterisation of the target gene(s) for TSCCs can be accomplished using our in-house cDNA microarray analysis combined with the qRT–PCR and immunohistochemistry, and that the Rab1A is a potential biomarker of tongue carcinogenesis. PMID:15870709

  14. DYRK1A inhibition as potential treatment for Alzheimer's disease.

    PubMed

    Stotani, Silvia; Giordanetto, Fabrizio; Medda, Federico

    2016-04-01

    In total, 47,500,000 people worldwide are affected by dementia and this number is estimated to double by 2030 and triple within 2050 resulting in a huge burden on public health. Alzheimer's disease (AD), a progressive neurodegenerative disorder, is the most common cause of dementia, accounting for 60-70% of all the cases. The cause of AD is still poorly understood but several brain abnormalities (e.g., loss of neuronal connections and neuronal death) have been identified in affected patients. In addition to the accumulation of β-amyloid plaques in the brain tissue, aberrant phosphorylation of tau proteins has proved to increase neuronal death. DYRK1A phosphorylates tau on 11 different Ser/Thr residues, resulting in the formation of aggregates called 'neurofibrillary tangles' which, together with amyloid plaques, could be responsible for dementia, neuronal degeneration and cell death. Small molecule inhibition of DYRK1A could thus represent an interesting approach toward the treatment of Alzheimer's and other neurodegenerative diseases. Herein we review the current progress in the identification and development of DYRK1A inhibitors. PMID:27073990

  15. ATP overflow in skeletal muscle 1A arterioles

    PubMed Central

    Kluess, Heidi A; Stone, Audrey J; Evanson, Kirk W

    2010-01-01

    The purpose of this study was to investigate the sources of ATP in the 1A arteriole, and to investigate age-related changes in ATP overflow. Arterioles (1A) from the red portion of the gastrocnemius muscle were isolated, cannulated and pressurized in a microvessel chamber with field stimulation electrodes. ATP overflow was determined using probes specific for ATP and null probes that were constructed similar to the ATP probes, but did not contain the enzyme coating. ATP concentrations were determined using a normal curve (0.78 to 25 μmol l−1 ATP). ATP overflow occurred in two phases. Phase one began in the first 20 s following stimulation and phase two started 35 s after field stimulation. Tetrodotoxin, a potent neurotoxin that blocks action potential generation in nerves, abolished both phases of ATP overflow. α1-Receptor blockade resulted in a small decrease in ATP overflow in phase two, but endothelial removal resulted in an increase in ATP overflow. ATP overflow was lowest in 6-month-old rats and highest in 12- and 2-month-old rats (P < 0.05). ATP overflow measured via biosensors was of neural origin with a small contribution from the vascular smooth muscle. The endothelium seems to play an important role in attenuating ATP overflow in 1A arterioles. PMID:20566660

  16. Energetics of the single-headed kinesin KIF1A

    NASA Astrophysics Data System (ADS)

    Kanada, Ryo; Sasaki, Kazuo

    2013-08-01

    KIF1A is a single-headed molecular motor that moves processively and unidirectionally along a microtubule by using the chemical energy released by hydrolyzing adenosine triphosphate (ATP) into adenosine diphosphate (ADP) and inorganic phosphate (Pi). Although the movement of KIF1A seems to have successfully been explained by a simple Brownian motor model of the flashing ratchet type, this model is not suited to discuss the energetics of KIF1A. We introduce an elaborated model of the ratchet type to investigate how the chemical free energy is converted into mechanical work by taking account of the binding and release of reactant (ATP) and product (ADP and Pi) molecules to and from the motor. The efficiency of energy transduction, the power output, and other quantities are calculated from the analytically obtained steady-state solution of the Fokker-Planck equations. It turns out that the concentrations of the reactant and product molecules that optimize both the efficiency and the power are close to those in the cell.

  17. Potential of Sentinel-1a for Grounding Line Measurements

    NASA Astrophysics Data System (ADS)

    Scheuchl, B.; Mouginot, J.; Rignot, E. J.

    2015-12-01

    The grounding line, the boundary between grounded and floating ice plays a crucial role for mass balance assessment, ice sheet modeling and the analysis of ice shelf melting. Interferometric Synthetic Aperture Radar data are the most accurate means to data to determine the grounding line on a large scale. The analysis of InSAR data from 1996 and 2011 shows a significant retreat of the grounding line in the Amundsen Sea Sector of West Antarctica along a retrograde bed. A new generation of spaceborne SAR sensors (Sentinel-1a, ALOS2) was launched in 2014 and has begun operational data collection since. Sentinel-1a collects data over ice sheets in a novel TOPSAR mode to provide large area coverage at relatively high resolution. Working closely with the ice sheet science community, ESA has implemented a data acquisition plan that ensures at least one ice sheet wide coverage per year and ongoing coverage in key coastal regions. The new mode, however does lead to some challenges in data processing. With focus on glaciers in West Antarctica (in particular we look at Smith, Pope and Kohler Glaciers), we show the potential of Sentinel-1a for grounding line mapping. Combining data from several currently available missions, we provide a 2015 update for the grounding line in the region.

  18. NASA Electronic Parts and Packaging Program

    NASA Technical Reports Server (NTRS)

    Kayali, Sammy

    2000-01-01

    NEPP program objectives are to: (1) Access the reliability of newly available electronic parts and packaging technologies for usage on NASA projects through validations, assessments, and characterizations, and the development of test methods/tools; (2)Expedite infusion paths for advanced (emerging) electronic parts and packaging technologies by evaluations of readiness for manufacturability and project usage consideration; (3) Provide NASA projects with technology selection, application, and validation guidelines for electronic parts and packaging hardware and processes; nd (4) Retain and disseminate electronic parts and packaging quality assurance, reliability validations, tools, and availability information to the NASA community.

  19. Metallic parts fabrication using the SIS process

    NASA Astrophysics Data System (ADS)

    Mojdeh, Mehdi

    Since early 1980s, quite a few techniques of Rapid Prototyping (RP), also known as Layered Manufacturing, have been developed. By building three-dimensional parts in a layer-by-layer additive manner, these techniques allow freeform fabrication of parts of complex geometry. Despite recent advances in fabrication of polymer parts, most of the existing rapid prototyping processes are still not capable of fabrication of accurate metallic parts with acceptable mechanical properties. Insufficient dimensional accuracy, limited number of materials, proper mechanical properties, required post machining and lack of repeatability between builds have greatly limited the market penetration of these techniques. This dissertation presents an innovative layered manufacturing technique for fabrication of dense metallic parts called Selective Inhibition Sintering (SIS), developed at the University of Southern California. The SIS-Metal technology adapts RP capabilities and extends them to the field of fabrication of metallic parts for a variety of applications such as tooling and low volume production. Using this process, a metallic part, with varying 3 dimensional geometries, can be automatically constructed from a wide range of materials. SIS-Metal is the only RP process which is suitable for fabrication of dense, complex shaped, accurate objects using a variety of materials. In the SIS-Metal process a metallic part is built layer by layer by deposition for each layer of an inhibitor material which defines the corresponding layer boundary and then filling the voids of the created geometry with metal powder; and compacting the layer formed to reach a high powder density. The resulting green part is then sintered in a furnace to yield the final functional part. In this research different inhibition techniques were explored and a series of single and multi layer parts was fabricated using the most promising inhibition technique, namely, macro-mechanical inhibition. Dimensional

  20. Reliability and quality EEE parts issues

    NASA Technical Reports Server (NTRS)

    Barney, Dan; Feigenbaum, Irwin

    1990-01-01

    NASA policy and procedures are established which govern the selection, testing, and application of electrical, electronic, and electromechanical (EEE) parts. Recent advances in the state-of-the-art of electronic parts and associated technologies can significantly impact the electronic designs and reliability of NASA space transportation avionics. Significant issues that result from these advances are examined, including: recent advances in microelectronics technology (as applied to or considered for use in NASA projects); electron packaging technology advances (concurrent with, and as a result of, the development of the advanced microelectronic devices); availability of parts used in space avionics; and standardization and integration of parts activities between projects, centers, and contractors.

  1. Opportunistic replacement of fusion power system parts

    SciTech Connect

    Day, J.A.; George, L.L.

    1981-10-26

    This paper describes a maintenance problem in a fusion power plant. The problem is to specify which life limited parts should be replaced when there is an opportunity. The objective is to minimize the cost rate of replacement parts and of maintenance actions while satisfying a power plant availability constraint. The maintenance policy is to look ahead and replace all parts that will reach their life limits within a time called a screen. Longer screens yield greater system availabilities because more parts are replaced prior to their life limits.

  2. Method of forming and assembly of metal parts and ceramic parts

    DOEpatents

    Ripley, Edward B.

    2011-11-22

    A method of forming and assembling at least two parts together that may be metal, ceramic, or a combination of metal and ceramic parts. Such parts may have different CTE. Individual parts that are formed and sintered from particles leave a network of interconnecting porosity in each sintered part. The separate parts are assembled together and then a fill material is infiltrated into the assembled parts using a method such as capillary action, gravity, and/or pressure. The assembly is then cured to yield a bonded and fully or near-fully dense part that has the desired physical and mechanical properties for the part's intended purpose. Structural strength may be added to the parts by the inclusion of fibrous materials.

  3. Overexpression of HSPA1A enhances the osteogenic differentiation of bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signaling pathway

    PubMed Central

    Zhang, Wei; Xue, Deting; Yin, Houfa; Wang, Shengdong; Li, Chao; Chen, Erman; Hu, Dongcai; Tao, Yiqing; Yu, Jiawei; Zheng, Qiang; Gao, Xiang; Pan, Zhijun

    2016-01-01

    HSPA1A, which encodes cognate heat shock protein 70, plays important roles in various cellular metabolic pathways. To investigate its effects on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), its expression level was compared between undifferentiated and differentiated BMSCs. Rat HSPA1A overexpression in BMSCs increased osteoblast-specific gene expression, alkaline phosphatase activity, and mineral deposition in vitro. Moreover, it upregulated β-catenin and downregulated DKK1 and SOST. The enhanced osteogenesis due to HSPA1A overexpression was partly rescued by a Wnt/β-catenin inhibitor. Additionally, using a rat tibial fracture model, a sheet of HSPA1A-overexpressing BMSCs improved bone fracture healing, as determined by imaging and histological analysis. Taken together, these findings suggest that HSPA1A overexpression enhances osteogenic differentiation of BMSCs, partly through Wnt/β-catenin. PMID:27279016

  4. Metrology of Large Parts. Chapter 5

    NASA Technical Reports Server (NTRS)

    Stahl, H. Philip

    2012-01-01

    As discussed in the first chapter of this book, there are many different methods to measure a part using optical technology. Chapter 2 discussed the use of machine vision to measure macroscopic features such as length and position, which was extended to the use of interferometry as a linear measurement tool in chapter 3, and laser or other trackers to find the relation of key points on large parts in chapter 4. This chapter looks at measuring large parts to optical tolerances in the sub-micron range using interferometry, ranging, and optical tools discussed in the previous chapters. The purpose of this chapter is not to discuss specific metrology tools (such as interferometers or gauges), but to describe a systems engineering approach to testing large parts. Issues such as material warpage and temperature drifts that may be insignificant when measuring a part to micron levels under a microscope, as will be discussed in later chapters, can prove to be very important when making the same measurement over a larger part. In this chapter, we will define a set of guiding principles for successfully overcoming these challenges and illustrate the application of these principles with real world examples. While these examples are drawn from specific large optical testing applications, they inform the problems associated with testing any large part to optical tolerances. Manufacturing today relies on micrometer level part performance. Fields such as energy and transportation are demanding higher tolerances to provide increased efficiencies and fuel savings. By looking at how the optics industry approaches sub-micrometer metrology, one can gain a better understanding of the metrology challenges for any larger part specified to micrometer tolerances. Testing large parts, whether optical components or precision structures, to optical tolerances is just like testing small parts, only harder. Identical with what one does for small parts, a metrologist tests large parts and optics

  5. Serotonin 5-HT1A receptors as targets for agents to treat psychiatric disorders: rationale and current status of research.

    PubMed

    Celada, Pau; Bortolozzi, Analía; Artigas, Francesc

    2013-09-01

    Psychiatric disorders represent a large economic burden in modern societies. However, pharmacological treatments are still far from optimal. Drugs used in the treatment of major depressive disorder (MDD) and anxiety disorders (selective serotonin [5-HT] reuptake inhibitors [SSRIs] and serotonin-noradrenaline reuptake inhibitors [SNRIs]) are pharmacological refinements of first-generation tricyclic drugs, discovered by serendipity, and show low efficacy and slowness of onset. Moreover, antipsychotic drugs are partly effective in positive symptoms of schizophrenia, yet they poorly treat negative symptoms and cognitive deficits. The present article reviews the neurobiological basis of 5-HT1A receptor (5-HT1A-R) function and the role of pre- and postsynaptic 5-HT1A-Rs in the treatment of MDD, anxiety and psychotic disorders. The activation of postsynaptic 5-HT1A-Rs in corticolimbic areas appears beneficial for the therapeutic action of antidepressant drugs. However, presynaptic 5-HT1A-Rs play a detrimental role in MDD, since individuals with high density or function of presynaptic 5-HT1A-Rs are more susceptible to mood disorders and suicide, and respond poorly to antidepressant drugs. Moreover, the indirect activation of presynaptic 5-HT1A-Rs by SSRIs/SNRIs reduces 5-HT neuron activity and terminal 5-HT release, thus opposing the elevation of extracellular 5-HT produced by blockade of the serotonin transporter (SERT) in the forebrain. Chronic antidepressant treatment desensitizes presynaptic 5-HT1A-Rs, thus reducing the effectiveness of the 5-HT1A autoreceptor-mediated negative feedback. The prevention of this process by the non-selective partial agonist pindolol accelerates clinical antidepressant effects. Two new antidepressant drugs, vilazodone (marketed in the USA) and vortioxetine (in development) incorporate partial 5-HT1A-R agonist properties with SERT blockade. Several studies with transgenic mice have also established the respective role of pre- and

  6. Search Engines for Tomorrow's Scholars, Part Two

    ERIC Educational Resources Information Center

    Fagan, Jody Condit

    2012-01-01

    This two-part article considers how well some of today's search tools support scholars' work. The first part of the article reviewed Google Scholar and Microsoft Academic Search using a modified version of Carole L. Palmer, Lauren C. Teffeau, and Carrier M. Pirmann's framework (2009). Microsoft Academic Search is a strong contender when…

  7. 40 CFR Appendix - Tables to Part 132

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 21 2010-07-01 2010-07-01 false Tables to Part 132 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS WATER QUALITY GUIDANCE FOR THE GREAT LAKES SYSTEM Application of part 132 requirements in Great Lakes States and Tribes. Pt. 132,...

  8. Part Timers and University Faculty Development.

    ERIC Educational Resources Information Center

    Dick, Robert C.

    The increasing number of part-time faculty, especially in university speech communication departments, appears to provide vital continuity in professional development. There are three kinds of part-time faculty: those with new doctoral degrees who want to teach full time but who cannot find appointments, those who are still active or semiactive…

  9. [Basic lung ultrasound. Part 2. Parenchymal diseases].

    PubMed

    de la Quintana Gordon, F B; Nacarino Alcorta, B; Fajardo Pérez, M

    2015-01-01

    In this second part, an analysis is made of the pathology of lung parenchyma. This text is structured into different sections, including the study of atelectasias, pneumonia and abscess, interstitial/alveolar or Blines patterns, and finally an analysis is made of pulmonary embolism. With this second part, the basic knowledge to develop lung ultrasound in the anesthesia department has been presented.

  10. International Perspectives in Leadership Development: Part I.

    PubMed

    Bleich, Michael R

    2015-08-01

    In this first part of a two-part series, leadership development perspectives are shared from interviews and experiences gained while visiting Taiwan and China. Each country has a distinctive culture and needs, yet both are rapidly rising to strengthen the leadership capacity of nurses and are increasingly a source of advances in global leadership and organizational science.

  11. Part-Time Learners Need Support Too

    ERIC Educational Resources Information Center

    Fazackerley, Anna

    2009-01-01

    In England, part-time students have to pay their fees up-front, they can't take out a government-supported loan, and their chances of securing any financial support are depressingly slim. While all full-time students are entitled to some state support, Parliamentary questions have revealed that nine out of 10 part-time students receive nothing at…

  12. Preliminary Report on Part-Time Faculty.

    ERIC Educational Resources Information Center

    California Community Colleges, Sacramento.

    This report summarizes survey data on part-time faculty utilization in California community colleges. Data was received from 67 of the 69 community college districts. The primary purpose of the survey was to obtain information that would be useful to community college boards and administrators in dealing with part-time faculty legislation. Tables…

  13. Teaching Science to ELLs, Part I

    ERIC Educational Resources Information Center

    Bautista, Nazan; Castaneda, Martha

    2011-01-01

    Since 1995, the population of English language learners (ELLs) in the United States has increased by an astonishing 57% (Maxwell 2009). Though ELLs come from many different backgrounds, they share the common challenge of learning English while responding to academic content demands. Part I of this two-part article provides key strategies every…

  14. FOUNDATIONS OF THAI. BOOK I, PART 1.

    ERIC Educational Resources Information Center

    ANTHONY, EDWARD M.; AND OTHERS

    THIS BEGINNING TEXT PRESENTS THE ESSENTIALS OF THAI PRONUNCIATION AND GRAMMAR IN A FORMAT GEARED TO REGULAR COLLEGE LANGUAGE COURSE SCHEDULING. THE COURSE CONSISTS OF TWO VOLUMES, BOOK I, PART 1 (LESSONS 1-14) AND BOOK I, PART 2 (LESSONS 15-25), PROVIDING MATERIAL FOR TWO UNIVERSITY SEMESTERS OF CLASSROOM AND LABORATORY WORK. BASED ON A…

  15. A Theory of Intellectual Development: Part 1.

    ERIC Educational Resources Information Center

    Confrey, Jere

    1994-01-01

    Part 1 of a three-part article analyzing radical constructivism (as one interpretation of Piaget) and the socio-cultural perspective (as one interpretation of Vygotsky), including major principles, primary contributions to mathematics education, and potential limitations. Introduces an integration of the two theories through a feminist…

  16. [Basic lung ultrasound. Part 2. Parenchymal diseases].

    PubMed

    de la Quintana Gordon, F B; Nacarino Alcorta, B; Fajardo Pérez, M

    2015-01-01

    In this second part, an analysis is made of the pathology of lung parenchyma. This text is structured into different sections, including the study of atelectasias, pneumonia and abscess, interstitial/alveolar or Blines patterns, and finally an analysis is made of pulmonary embolism. With this second part, the basic knowledge to develop lung ultrasound in the anesthesia department has been presented. PMID:25708093

  17. Fixture for mounting small parts for processing

    DOEpatents

    Foreman, Larry R.; Gomez, Veronica M.; Thomas, Michael H.

    1990-01-01

    A fixture for mounting small parts, such as fusion target spheres or microelectronic components. A glass stalk is drawn and truncated near its tip. The truncated end of the glass stalk is dipped into silicone rubber forming an extending streamer. After the rubber cures for approximately 24 hours, a small part is touched to the streamer, and will be held securely throughout processing.

  18. Urban versus Rural: Part-Time Enrollment

    ERIC Educational Resources Information Center

    Copeland, Michael; Tietjen-Smith, Tara; Waller, Lee Rusty; Waller, Sharon Kay

    2008-01-01

    The researchers examined part-time enrollment within public two-year, degree-granting community colleges by the degree of urbanization classifications of city, suburban, town, and rural for fall 2003 and 2005. Findings indicate no statistical differences in part-time enrollment between city and suburban institutions. No statistical differences…

  19. 30 CFR 57.19122 - Replacement parts.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE SAFETY AND HEALTH SAFETY AND HEALTH STANDARDS-UNDERGROUND METAL AND NONMETAL MINES Personnel Hoisting Inspection and Maintenance § 57.19122 Replacement parts. Parts used to repair hoists shall have...

  20. 30 CFR 56.19122 - Replacement parts.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE SAFETY AND HEALTH SAFETY AND HEALTH STANDARDS-SURFACE METAL AND NONMETAL MINES Personnel Hoisting Inspection and Maintenance § 56.19122 Replacement parts. Parts used to repair hoists shall have...

  1. 49 CFR Appendix - Figures to Part 38

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 1 2013-10-01 2013-10-01 false Figures to Part 38 Transportation Office of the Secretary of Transportation AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY SPECIFICATIONS FOR TRANSPORTATION VEHICLES Other Vehicles and Systems Trams, and similar vehicles, and systems Pt. 38, Figures Figures to Part 38 ER28SE98.000...

  2. 49 CFR Appendix - Figures to Part 38

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 1 2012-10-01 2012-10-01 false Figures to Part 38 Transportation Office of the Secretary of Transportation AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY SPECIFICATIONS FOR TRANSPORTATION VEHICLES Other Vehicles and Systems Trams, and similar vehicles, and systems Pt. 38, Figures Figures to Part 38 ER28SE98.000...

  3. Basic Principles of Lyophilization, Part 2.

    PubMed

    Akers, Michael J

    2016-01-01

    The achievement of a high-quality lyophilized (freeze-dried) dosage form involves the combination of optimal formulation design and optimal freeze-dry cycle design. Part 1 of this 2-part article discussed the basic principles and procedures of lyophilization up to a discussion on the different stages of lyophilization. The stages of lyophilization are discussed within this article.

  4. Part Time Study in Higher Education.

    ERIC Educational Resources Information Center

    Neville, Colin

    This document, which is intended for adults considering enrolling in part-time study in higher education, examines the objectives and experiences of adults who have pursued part-time study in higher education in the United Kingdom. The following reasons why adults return to higher education are discussed: personal development; self-fulfillment;…

  5. 47 CFR Parts 401-499 - [RESERVED

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 5 2014-10-01 2014-10-01 false 401 PARTS 401-499 Telecommunication NATIONAL TELECOMMUNICATIONS AND INFORMATION ADMINISTRATION, DEPARTMENT OF COMMERCE, AND NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION E-911 GRANT PROGRAM Closeout. PARTS 401-499...

  6. Actel Parts Usage in GSFC Projects

    NASA Technical Reports Server (NTRS)

    Sahu, Kusum

    2006-01-01

    The purpose of this paper is to provide an overview of the Actel field programmable gate array (FPGA) issues and investigations to date, provide information to GSFC parts engineers on the risks associated with the use of these parts in flight, provide procurement options for their respective projects, and provide general guidance for use of these devices if no other acceptable options exist.

  7. Body Parts and Early-Learned Verbs

    ERIC Educational Resources Information Center

    Maouene, Josita; Hidaka, Shohei; Smith, Linda B.

    2008-01-01

    This article reports the structure of associations among 101 common verbs and body parts. The verbs are those typically learned by children learning English prior to 3 years of age. In a free association task, 50 adults were asked to provide the single body part that came to mind when they thought of each verb. Analyses reveal highly systematic…

  8. Part and Bipartial Canonical Correlation Analysis.

    ERIC Educational Resources Information Center

    Timm, Neil H.; Carlson, James E.

    Part and bi-partial canonical correlations were developed by extending the definitions of part and bi-partial correlation to sets of variates. These coefficients may be used to help researchers explore relationships which exist among several sets of normally distributed variates. (Author)

  9. 49 CFR Appendix - Figures to Part 38

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false Figures to Part 38 Transportation Office of the Secretary of Transportation AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY SPECIFICATIONS FOR TRANSPORTATION VEHICLES Other Vehicles and Systems Trams, and similar vehicles, and systems Pt. 38, Figures Figures to Part 38 ER28SE98.000...

  10. 49 CFR Appendix - Figures to Part 38

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Figures to Part 38 Transportation Office of the Secretary of Transportation AMERICANS WITH DISABILITIES ACT (ADA) ACCESSIBILITY SPECIFICATIONS FOR TRANSPORTATION VEHICLES Other Vehicles and Systems Trams, and similar vehicles, and systems Pt. 38, Figures Figures to Part 38 ER28SE98.000...

  11. 30 CFR 56.19122 - Replacement parts.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE SAFETY AND HEALTH SAFETY AND HEALTH STANDARDS-SURFACE METAL AND NONMETAL MINES Personnel Hoisting Inspection and Maintenance § 56.19122 Replacement parts. Parts used to repair hoists shall have...

  12. 30 CFR 56.19122 - Replacement parts.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE SAFETY AND HEALTH SAFETY AND HEALTH STANDARDS-SURFACE METAL AND NONMETAL MINES Personnel Hoisting Inspection and Maintenance § 56.19122 Replacement parts. Parts used to repair hoists shall have...

  13. 30 CFR 57.19122 - Replacement parts.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR METAL AND NONMETAL MINE SAFETY AND HEALTH SAFETY AND HEALTH STANDARDS-UNDERGROUND METAL AND NONMETAL MINES Personnel Hoisting Inspection and Maintenance § 57.19122 Replacement parts. Parts used to repair hoists shall have...

  14. 30 CFR 57.19122 - Replacement parts.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

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    Code of Federal Regulations, 2011 CFR

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  16. Project Management; Part 1--The Soft Skills.

    ERIC Educational Resources Information Center

    Bailey, Harold J.; Ergott, Kathleen A.

    1998-01-01

    Presents the first of a three-part series of articles on the life of a project manager. Discussion in Part 1 deals with the people and related skills involved in the project, including: interrelationships between the client, project manager, and executive management; oral, written, and body-language communication forms; and planning and…

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    Code of Federal Regulations, 2013 CFR

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    Code of Federal Regulations, 2013 CFR

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    Code of Federal Regulations, 2014 CFR

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    Code of Federal Regulations, 2010 CFR

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