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Sample records for 1b high performance

  1. Intralaboratory development and evaluation of a high-performance liquid chromatography-fluorescence method for detection and quantitation of aflatoxins M1, B1, B2, G1, and G2 in animal liver.

    PubMed

    Shao, Dahai; Imerman, Paula M; Schrunk, Dwayne E; Ensley, Steve M; Rumbeiha, Wilson K

    2016-11-01

    Aflatoxins are potent mycotoxins with effects that include hepatotoxicity, immunosuppression, and suppression of animal growth and production. The etiologic diagnosis of aflatoxicosis, which is largely based on analysis of contaminated feed matrices, has significant disadvantages given the fact that representative feed samples may not be available and feed-based test methods are not confirmatory of an etiologic diagnosis. A tissue-based analytical method for biomarkers of exposure would be valuable for confirmation of aflatoxicosis. We describe in-house development and evaluation of a high-performance liquid chromatographic method with fluorescence detection and precolumn derivatization for determination of aflatoxins M1, B1, B2, G1, and G2 in animal liver. The method demonstrates good selectivity for the tested aflatoxins in the liver matrix. The overall range was 0.03-0.10 ng/g for limit of detection and 0.09-0.18 ng/g for limit of quantitation. The correlation coefficient (R(2)) of calibration curves was >0.9978 for AFM1, 0.9995 for AFB1, 0.9986 for AFB2, 0.9983 for AFG1, and 0.9980 for AFG2 For fortification levels of 0.2-10 ng/g, repeatability was 10-18% for AFM1, 7-14% for AFB1, 5-14% for AFB2, 6-16% for AFG1, and 10-15% for AFG2 Recovery was 52-57% for AFM1, 54-62% for AFB1, 55-61% for AFB2, 57-67% for AFG1, and 61-65% for AFG2 There was no liver matrix effect found. The method is rugged against minor changes based on the selected factors. The results indicate that the proposed method is suitable for quantitative determination of aflatoxins M1, B1, B2, G1, and G2 in liver. © 2016 The Author(s).

  2. Escitalopram reduces attentional performance in anxious older adults with high-expression genetic variants at serotonin 2A and 1B receptors

    PubMed Central

    Lenze, Eric J.; Dixon, David; Nowotny, Petra; Lotrich, Francis E.; Doré, Peter M.; Pollock, Bruce G.; Hinrichs, Anthony L.; Butters, Meryl A.

    2014-01-01

    Older adults are among the most vulnerable to adverse cognitive effects of psychotropic medications and, therefore, the personalization of psychotropic treatment based on adverse drug reactions in this demographic is of great importance. We examined changes on neuropsychological tests of attention attributable to selective serotonin reuptake inhibitor (SSRI) treatment in anxious older adults. We also examined whether variation in serotonin receptor genes was associated with reduced attentional performance with SSRIs. We examined change from pre- to post-treatment in two attention measures – digit span and coding – in 133 adults aged ≥60 yr with generalized anxiety disorder in a 12-wk trial of escitalopram vs. placebo. We also examined attentional change in relation to genetic variability in four central serotonin receptors: the serotonin transporter and serotonin 1A, 2A and 1B receptors. Digit span scores were significantly lowered in patients receiving escitalopram relative to placebo, indicating reduced attentional performance attributable to the SSRI. Individuals with high-transcription variants in the receptors 5-HTR2A rs6311 and 5-HTR1B rs11568817 had greater reductions in attention with SSRI treatment compared to placebo. We conclude that SSRIs reduce attention in older adults, particularly in those with high-expression genetic variants at the serotonin 2A and 1B receptors. Analysing neuropsychological changes with SSRIs in relation to genetic variation in the serotonin system may be a useful strategy for detecting subgroups of older adults who are more susceptible to side-effects of SSRIs. These results, if confirmed, could lead to the personalization of SSRI use to reduce adverse neurocognitive effects. PMID:22717018

  3. Escitalopram reduces attentional performance in anxious older adults with high-expression genetic variants at serotonin 2A and 1B receptors.

    PubMed

    Lenze, Eric J; Dixon, David; Nowotny, Petra; Lotrich, Francis E; Doré, Peter M; Pollock, Bruce G; Hinrichs, Anthony L; Butters, Meryl A

    2013-03-01

    Older adults are among the most vulnerable to adverse cognitive effects of psychotropic medications and, therefore, the personalization of psychotropic treatment based on adverse drug reactions in this demographic is of great importance. We examined changes on neuropsychological tests of attention attributable to selective serotonin reuptake inhibitor (SSRI) treatment in anxious older adults. We also examined whether variation in serotonin receptor genes was associated with reduced attentional performance with SSRIs. We examined change from pre- to post-treatment in two attention measures - digit span and coding - in 133 adults aged ≥60 yr with generalized anxiety disorder in a 12-wk trial of escitalopram vs. placebo. We also examined attentional change in relation to genetic variability in four central serotonin receptors: the serotonin transporter and serotonin 1A, 2A and 1B receptors. Digit span scores were significantly lowered in patients receiving escitalopram relative to placebo, indicating reduced attentional performance attributable to the SSRI. Individuals with high-transcription variants in the receptors 5-HTR2A rs6311 and 5-HTR1B rs11568817 had greater reductions in attention with SSRI treatment compared to placebo. We conclude that SSRIs reduce attention in older adults, particularly in those with high-expression genetic variants at the serotonin 2A and 1B receptors. Analysing neuropsychological changes with SSRIs in relation to genetic variation in the serotonin system may be a useful strategy for detecting subgroups of older adults who are more susceptible to side-effects of SSRIs. These results, if confirmed, could lead to the personalization of SSRI use to reduce adverse neurocognitive effects.

  4. [Determination of aflatoxin B1, B2, G1, G2 in armeniacae semen amarum by high-performance liquid chromatography-tandem mass spectrometry].

    PubMed

    Zheng, Run-Sheng; Xu, Hui; Wang, Wen-Li; Zhan, Ruo-Ting; Chen, Wei-Wen

    2013-10-01

    A simple, rapid and cost-effective high-performance liquid chromatography-tandem mass spectrometry (LC-MS/ MS) method was established for simultaneous determination of aflatoxins (AFB1, AFB2, AFG1, AFG2) in Armeniacae Semen Amarum and the application was performance in 11 samples collected from different markets, medical stores and hospitals. The sample was extracted with 84% acetonitrile/water and 250 microL extraction was directly injected into a LC-MS/MS system without further purification procedure after being redissolved with methanol. The LC separation was performed on a C18 column with a linear gradient elution program of 4 mmol x L(-1) NH4 Ac-0.1% formic acid solution and menthol as the mobile phase. Selected reaction monitoring (SRM) was used for selective determination of the four aflatoxins on a triple quadruple mass spectrometer, which was operated in positive ionization modes. All the four aflatoxins showed a good linear relationship with r > 0.999 0, the average recoveries were between 87.88% and 102.9% and the matrix effect was ranged from 90.71% to 99.30% in low, intermediate and high levels. Furthermore, the higher recovery was obtained by the method reported in this study, comparing to the cleanup procedure with the Mycosep 226 purification column. Eleven samples collected were detected and the contamination levels of the AFB1 were between 1.590-2.340 microg x kg(-1) and the AF (B1 + B2 + G1 + G2) was ranged from 2.340 to 3.340 microg x kg(-1). In summary, the developed method was suitable to detect and screen AFB1, AFB2, AFG1, AFG2 in Armeniacae Semen Amarum.

  5. Level1B Performance Evaluation On CryoSat Products

    NASA Astrophysics Data System (ADS)

    Scagliola, Michele; Fornari, Marco; Tagliani, Nicolas

    2013-12-01

    A performance assessment of the CryoSat products for SAR/SARin modes is here presented. The effective across-track IRF has been compared with the expected one, according to the system requirements, and exploiting the calibration products. Then, starting from the along-track processing for Delay/Doppler Radar altimeters, the theoretical model of the along-track IRF for CryoSat has been derived. Moreover the effects of the azimuth windowing on the along-track resolution and on the clutter suppression have been investigated. In particular, the clutter has been evaluated on the Level1b products for both sea ice and ocean scenario, revealing that the azimuth window allows to improve the signal to clutter ratio also in case of ocean waveforms.

  6. [A high throughput coupled with high performance liquid chromatography-tandem mass spectrometry method for determination of aflatoxin B1, B2, G1, G2 in 10 traditional Chinese medicines].

    PubMed

    Zheng, Run-Sheng; Xu, Hui; Peng, Yuan-Xia; Wang, Wen-Li; Zhan, Ruo-Ting; Chen, Wei-Wen

    2014-01-01

    As the dilution procedure was applied, a simple, rapid and cost-effective high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of aflatoxin B1, B2, G1, and G2 was successfully by performed in a total 83 samples of 10 traditional Chinese medicines (TCMs), which were collected from 5 different hospital pharmacies and 5 different medical stores in Guangzhou city. Matrix effects of these 10 TCMs were ranged from 80.23% to 115.5% in low, intermediate and high concentration levels, indicating that the negative effect was overcome in this study. Meanwhile, the analysis method was proved to be stable and reliable during the whole analysis using Semen Armeniacae Amarum spiked 3 concentration levels of standard solution as quality control samples and the RSD < 6.6% was obtained. The contamination levels of 83 investigated samples were 13.89% and 17.02% in hospital pharmacies and medical stores, respectively. The result was presented to provide relevant reference and supplement to those researchers in TCMs analysis and screening.

  7. An ultra-high-performance liquid chromatography-tandem mass spectrometry method for simultaneous determination of aflatoxins B1, B2, G1, G2, M1 and M2 in traditional Chinese medicines.

    PubMed

    Han, Zheng; Zheng, Yunliang; Luan, Lianjun; Cai, Zengxuan; Ren, Yiping; Wu, Yongjiang

    2010-04-07

    An ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of aflatoxins B1, B2, G1, G2, M1 and M2 in traditional Chinese medicines (TCMs) was developed. The approach was characterized in details and a special focus was placed on the recovery rates of isolation procedure in different TCM matrices, i.e. rhizomes and roots, seeds, flowers, grasses and leaves. For this purpose, [(13)C(17)]-aflatoxinB1 was employed as the internal standard and a reliable solid phase extraction-based clean-up method was developed. The observed recovery rates of the six aflatoxins ranged from 85.6% to 117.6% in different matrices. Then, the established method was successfully applied to the determination of the six aflatoxins in various TCMs. For 30 commercial samples analyzed, 16 were contaminated with aflatoxins. The mean levels (incidence) of aflatoxins B1, B2, G1 and G2 in positive samples were 1.40 (68.8%), 1.27 (50.0%), 0.50 (43.8%) and 0.94 (43.8%) microg kg(-1), respectively. Interestingly, aflatoxin M1 was detected in two samples with the maximal content of 0.70 microg kg(-1). No sample was contaminated with aflatoxin M2. Meanwhile, a possible association between the contamination levels and the selected herbs was clarified in the present study.

  8. A high-conductance chemo-optogenetic system based on the vertebrate channel Trpa1b.

    PubMed

    Lam, Pui-Ying; Mendu, Suresh K; Mills, Robert W; Zheng, Baohui; Padilla, Hugo; Milan, David J; Desai, Bimal N; Peterson, Randall T

    2017-09-19

    Optogenetics is a powerful research approach that allows localized optical modulation of selected cells within an animal via the expression of genetically encoded photo-excitable ion channels. Commonly used optogenetic techniques rely on the expression of microbial opsin variants, which have many excellent features but suffer from various degrees of blue spectral overlap and limited channel conductance. Here, we expand the optogenetics toolbox in the form of a tunable, high-conductance vertebrate cation channel, zTrpa1b, coupled with photo-activated channel ligands, such as optovin and 4g6. Our results demonstrate that zTrpa1b/ligand pairing offers high light sensitivity, millisecond-scale response latency in vivo, as well as adjustable channel off latency. Exogenous in vivo expression of zTrpa1b in sensory neurons allowed subcellular photo-activation, enabling light-dependent motor control. zTrpa1b/ligand was also suitable for cardiomyocyte pacing, as shown in experiments performed on zebrafish hearts in vivo as well as in human stem cell-derived cardiomyocytes in vitro. Therefore, zTrpa1b/optovin represents a novel tool for flexible, high-conductance optogenetics.

  9. Development and validation of ultra-high-performance liquid chromatography-tandem mass spectrometry methods for the simultaneous determination of beauvericin, enniatins (A, A1, B, B1) and cereulide in maize, wheat, pasta and rice.

    PubMed

    Decleer, Marlies; Rajkovic, Andreja; Sas, Benedikt; Madder, Annemieke; De Saeger, Sarah

    2016-11-11

    Rapid and accurate UPLC-MS/MS methods for the simultaneous determination of beauvericin and the related enniatins (A, A1, B, B1), together with cereulide were successfully developed and validated in cereal and cereal-based food matrices such as wheat, maize, rice and pasta. Although these emerging foodborne toxins are of different microbial origin, the similar structural, toxicological and food safety features provided rationale for their concurrent detection in relevant food matrices. A Waters Acquity UPLC system coupled to a Waters Quattro Premier XE™ Mass Spectrometer operating in ESI+ mode was employed. Sample pretreatment involved a fast and simple liquid extraction of the target toxins without any further clean-up step. For all toxins the sample preparation resulted in acceptable extraction recoveries with values of 85-105% for wheat, 87-106% for maize, 84-106% for rice and 85-105% for pasta. The efficient extraction protocol, together with a fast chromatographic separation of 7min allowed substantial saving costs and time showing its robustness and performance. The validation of the developed method was performed based on Commission Decision 2002/657/EC. The obtained limits of detection ranged from 0.1 to 1.0μgkg(-1) and the limits of quantification from 0.3 to 2.9μgkg(-1) for the targeted toxins in the selected matrices. The obtained sensitivities allow detection of relevant toxicological concentrations. All relative standard deviations for repeatability (intra-day) and intermediate precision (inter-day) were lower than 20%. Trueness, expressed as the apparent recovery varied from 80 to 107%. The highly sensitive and repeatable validated method was applied to 57 naturally contaminated samples allowing detection of sub-clinical doses of the toxins. Copyright © 2016 Elsevier B.V. All rights reserved.

  10. Simultaneous determination of aflatoxins B1, B2, G1, G2, M1 and M2 in peanuts and their derivative products by ultra-high-performance liquid chromatography-tandem mass spectrometry.

    PubMed

    Huang, Baifen; Han, Zheng; Cai, Zengxuan; Wu, Yongjiang; Ren, Yiping

    2010-03-03

    A reliable ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of aflatoxins B1, B2, G1, G2, M1 and M2 in peanuts and their derivative products was developed. The sample was extracted by 84% of acetonitrile aqueous solution and the extract was purified by a reliable solid phase extraction-based clean-up method. Then, the analytes were separated on Acquity UPLC HSS T3 column (100 mm x 2.1 mm, 1.8 microm particle size), and eluted with a mobile phase consisting of (A) water containing 0.1% formic acid and (B) acetonitrile/methanol (50/50, v/v). The separated compounds were detected with a Waters Micromass Quattro Ultima Pt tandem quadrupole mass spectrometer operating in positive electro-spray ionization using multiple reaction monitoring mode. The established method was extensively validated by determining the linearity (R(2) > or = 0.9990), average recovery (74.7-86.8%) and precision (relative standard deviation < or = 10.9%). It was shown to be a suitable method for simultaneous determination of the six aflatoxins in peanuts and their derivative products. Finally, a total of 73 samples randomly collected from different areas in Zhejiang province were screened for aflatoxins with the proposed method. The results showed that 31 samples of peanut butter, 14 samples of fresh peanut and 5 samples of musty peanut were contaminated with aflatoxins. Meanwhile, this was the first report on aflatoxins M1 and M2, which were found in unprocessed peanuts and their derivative products.

  11. Identification of Small Molecule Inhibitors of Jumonji AT-rich Interactive Domain 1B (JARID1B) Histone Demethylase by a Sensitive High Throughput Screen*

    PubMed Central

    Sayegh, Joyce; Cao, Jian; Zou, Mike Ran; Morales, Alfonso; Blair, Lauren P.; Norcia, Michael; Hoyer, Denton; Tackett, Alan J.; Merkel, Jane S.; Yan, Qin

    2013-01-01

    JARID1B (also known as KDM5B or PLU1) is a member of the JARID1 family of histone lysine demethylases responsible for the demethylation of trimethylated lysine 27 in histone H3 (H3K4me3), a mark for actively transcribed genes. JARID1B is overexpressed in several cancers, including breast cancer, prostate cancer, and lung cancer. In addition, JARID1B is required for mammary tumor formation in syngeneic or xenograft mouse models. JARID1B-expressing melanoma cells are associated with increased self-renewal character. Therefore, JARID1B represents an attractive target for cancer therapy. Here we characterized JARID1B using a homogeneous luminescence-based demethylase assay. We then conducted a high throughput screen of over 15,000 small molecules to identify inhibitors of JARID1B. From this screen, we identified several known JmjC histone demethylase inhibitors, including 2,4-pyridinedicarboxylic acid and catechols. More importantly, we identified several novel inhibitors, including 2-4(4-methylphenyl)-1,2-benzisothiazol-3(2H)-one (PBIT), which inhibits JARID1B with an IC50 of about 3 μm in vitro. Consistent with this, PBIT treatment inhibited removal of H3K4me3 by JARID1B in cells. Furthermore, this compound inhibited proliferation of cells expressing higher levels of JARID1B. These results suggest that this novel small molecule inhibitor is a lead compound that can be further optimized for cancer therapy. PMID:23408432

  12. B-2 Extremely High Frequency SATCOM and Computer Increment 1 (B-2 EHF Inc 1)

    DTIC Science & Technology

    2015-12-01

    Selected Acquisition Report (SAR) RCS: DD-A&T(Q&A)823-224 B-2 Extremely High Frequency SATCOM and Computer Increment 1 (B-2 EHF Inc 1) As of FY...Officer PM - Program Manager POE - Program Office Estimate RDT&E - Research, Development, Test, and Evaluation SAR - Selected Acquisition Report SCP

  13. STAR and AKR1B10 are down-regulated in high-grade endometrial cancer.

    PubMed

    Sinreih, Maša; Štupar, Saša; Čemažar, Luka; Verdenik, Ivan; Frković Grazio, Snježana; Smrkolj, Špela; Rižner, Tea Lanišnik

    2017-02-21

    Endometrial cancer is the most frequent gynecological malignancy in the developed world. The majority of cases are estrogen dependent, and are associated with diminished protective effects of progesterone. Endometrial cancer is also related to enhanced inflammation and decreased differentiation. In our previous studies, we examined the expression of genes involved in estrogen and progesterone actions in inflammation and tumor differentiation, in tissue samples from endometrial cancer and adjacent control endometrium. The aims of the current study were to examine correlations between gene expression and several demographic characteristics, and to evaluate changes in gene expression with regard to histopathological and clinical characteristics of 51 patients. We studied correlations and differences in expression of 38 genes involved in five pathophysiological processes: (i) estrogen-stimulated proliferation; (ii) estrogen-dependent carcinogenesis; (iii) diminished biosynthesis of progesterone: (iv) enhanced formation of progesterone metabolites; and (v) increased inflammation and decreased differentiation. Spearman correlation coefficient analysis shows that expression of PAQR7 correlates with age, expression of SRD5A1, AKR1B1 and AKR1B10 correlate with body mass, while expression of SRD5A1 and AKR1B10 correlate with body mass index. When patients with endometrial cancer were stratified based on menopausal status, histological grade, myometrial invasion, lymphovascular invasion, and FIGO stage, Mann-Whitney U tests revealed significantly decreased expression of STAR (4.4-fold; adjusted p=0.009) and AKR1B10 (9-fold; adjusted p=0.003) in high grade versus low grade tumors. Lower levels of STAR might lead to decreased de-novo steroid hormone synthesis and tumor differentiation, and lower levels of AKR1B10 to diminished elimination of toxic electrophilic carbonyl compounds in high-grade endometrial cancer. These data thus reveal the potential of STAR and AKR1B10 as

  14. Pathogenesis-Related Protein 1b1 (PR1b1) Is a Major Tomato Fruit Protein Responsive to Chilling Temperature and Upregulated in High Polyamine Transgenic Genotypes.

    PubMed

    Goyal, Ravinder K; Fatima, Tahira; Topuz, Muhamet; Bernadec, Anne; Sicher, Richard; Handa, Avtar K; Mattoo, Autar K

    2016-01-01

    Plants execute an array of mechanisms in response to stress which include upregulation of defense-related proteins and changes in specific metabolites. Polyamines - putrescine (Put), spermidine (Spd), and spermine (Spm) - are metabolites commonly found associated with abiotic stresses such as chilling stress. We have generated two transgenic tomato lines (556HO and 579HO) that express yeast S-adenosylmethionine decarboxylase and specifically accumulate Spd and Spm in fruits in comparison to fruits from control (556AZ) plants (Mehta et al., 2002). Tomato fruits undergo chilling injury at temperatures below 13°C. The high Spd and Spm tomato together with the control azygous line were utilized to address role(s) of polyamines in chilling-injury signaling. Exposure to chilling temperature (2°C) led to several-fold increase in the Put content in all the lines. Upon re-warming of the fruits at 20°C, the levels of Spd and Spm increased further in the fruit of the two transgenic lines, the higher levels remaining stable for 15 days after re-warming as compared to the fruit from the control line. Profiling their steady state proteins before and after re-warming highlighted a protein of ∼14 kD. Using proteomics approach, protein sequencing and immunoblotting, the ∼14-kD protein was identified as the pathogenesis related protein 1b1 (PR1b1). The PR1b1 protein accumulated transiently in the control fruit whose level was barely detectable at d 15 post-warming while in the fruit from both the 556HO and 579HO transgenic lines PR1b1 abundance increased and remained stable till d 15 post warming. PR1b1 gene transcripts were found low in the control fruit with a visible accumulation only on d 15 post warming; however, in both the transgenic lines it accumulated and increased soon after rewarming being several-fold higher on day 2 while in 556HO line this increase continued until d 6 than the control fruit. The chilling-induced increase in PR1b1 protein seems independent of

  15. Pathogenesis-Related Protein 1b1 (PR1b1) Is a Major Tomato Fruit Protein Responsive to Chilling Temperature and Upregulated in High Polyamine Transgenic Genotypes

    PubMed Central

    Fatima, Tahira; Topuz, Muhamet; Bernadec, Anne; Sicher, Richard; Handa, Avtar K.; Mattoo, Autar K.

    2016-01-01

    Plants execute an array of mechanisms in response to stress which include upregulation of defense-related proteins and changes in specific metabolites. Polyamines – putrescine (Put), spermidine (Spd), and spermine (Spm) – are metabolites commonly found associated with abiotic stresses such as chilling stress. We have generated two transgenic tomato lines (556HO and 579HO) that express yeast S-adenosylmethionine decarboxylase and specifically accumulate Spd and Spm in fruits in comparison to fruits from control (556AZ) plants (Mehta et al., 2002). Tomato fruits undergo chilling injury at temperatures below 13°C. The high Spd and Spm tomato together with the control azygous line were utilized to address role(s) of polyamines in chilling-injury signaling. Exposure to chilling temperature (2°C) led to several-fold increase in the Put content in all the lines. Upon re-warming of the fruits at 20°C, the levels of Spd and Spm increased further in the fruit of the two transgenic lines, the higher levels remaining stable for 15 days after re-warming as compared to the fruit from the control line. Profiling their steady state proteins before and after re-warming highlighted a protein of ∼14 kD. Using proteomics approach, protein sequencing and immunoblotting, the ∼14-kD protein was identified as the pathogenesis related protein 1b1 (PR1b1). The PR1b1 protein accumulated transiently in the control fruit whose level was barely detectable at d 15 post-warming while in the fruit from both the 556HO and 579HO transgenic lines PR1b1 abundance increased and remained stable till d 15 post warming. PR1b1 gene transcripts were found low in the control fruit with a visible accumulation only on d 15 post warming; however, in both the transgenic lines it accumulated and increased soon after rewarming being several-fold higher on day 2 while in 556HO line this increase continued until d 6 than the control fruit. The chilling-induced increase in PR1b1 protein seems independent

  16. High diversity and no significant selection signal of human ADH1B gene in Tibet

    PubMed Central

    2012-01-01

    Background ADH1B is one of the most studied human genes with many polymorphic sites. One of the single nucleotide polymorphism (SNP), rs1229984, coding for the Arg48His substitution, have been associated with many serious diseases including alcoholism and cancers of the digestive system. The derived allele, ADH1B*48His, reaches high frequency only in East Asia and Southwest Asia, and is highly associated with agriculture. Micro-evolutionary study has defined seven haplogroups for ADH1B based on seven SNPs encompassing the gene. Three of those haplogroups, H5, H6, and H7, contain the ADH1B*48His allele. H5 occurs in Southwest Asia and the other two are found in East Asia. H7 is derived from H6 by the derived allele of rs3811801. The H7 haplotype has been shown to have undergone significant positive selection in Han Chinese, Hmong, Koreans, Japanese, Khazak, Mongols, and so on. Methods In the present study, we tested whether Tibetans also showed evidence for selection by typing 23 SNPs in the region covering the ADH1B gene in 1,175 individuals from 12 Tibetan populations representing all districts of the Tibet Autonomous Region. Multiple statistics were estimated to examine the gene diversities and positive selection signals among the Tibetans and other populations in East Asia. Results The larger Tibetan populations (Qamdo, Lhasa, Nagqu, Nyingchi, Shannan, and Shigatse) comprised mostly farmers, have around 12% of H7, and 2% of H6. The smaller populations, living on hunting or recently switched to farming, have lower H7 frequencies (Tingri 9%, Gongbo 8%, Monba and Sherpa 6%). Luoba (2%) and Deng (0%) have even lower frequencies. Long-range haplotype analyses revealed very weak signals of positive selection for H7 among Tibetans. Interestingly, the haplotype diversity of H7 is higher in Tibetans than in any other populations studied, indicating a longer diversification history for that haplogroup in Tibetans. Network analysis on the long-range haplotypes revealed

  17. Spitzer and z' secondary eclipse observations of the highly irradiated transiting brown dwarf KELT-1b

    SciTech Connect

    Beatty, Thomas G.; Gaudi, B. Scott; Collins, Karen A.; Kielkopf, John F.; Fortney, Jonathan; Knutson, Heather; Bruns, Jacob M.; Showman, Adam P.; Eastman, Jason; Pepper, Joshua; Siverd, Robert J.; Stassun, Keivan G.

    2014-03-10

    We present secondary eclipse observations of the highly irradiated transiting brown dwarf KELT-1b. These observations represent the first constraints on the atmospheric dynamics of a highly irradiated brown dwarf, the atmospheres of irradiated giant planets at high surface gravity, and the atmospheres of brown dwarfs that are dominated by external, rather than internal, energy. Using the Spitzer Space Telescope, we measure secondary eclipse depths of 0.195% ± 0.010% at 3.6 μm and 0.200% ± 0.012% at 4.5 μm. We also find tentative evidence for the secondary eclipse in the z' band with a depth of 0.049% ± 0.023%. These measured eclipse depths are most consistent with an atmosphere model in which there is a strong substellar hotspot, implying that heat redistribution in the atmosphere of KELT-1b is low. While models with a more mild hotspot or even with dayside heat redistribution are only marginally disfavored, models with complete heat redistribution are strongly ruled out. The eclipse depths also prefer an atmosphere with no TiO inversion layer, although a model with TiO inversion is permitted in the dayside heat redistribution case, and we consider the possibility of a day-night TiO cold trap in this object. For the first time, we compare the IRAC colors of brown dwarfs and hot Jupiters as a function of effective temperature. Importantly, our measurements reveal that KELT-1b has a [3.6] – [4.5] color of 0.07 ± 0.11, identical to that of isolated brown dwarfs of similarly high temperature. In contrast, hot Jupiters generally show redder [3.6] – [4.5] colors of ∼0.4, with a very large range from ∼0 to ∼1. Evidently, despite being more similar to hot Jupiters than to isolated brown dwarfs in terms of external forcing of the atmosphere by stellar insolation, KELT-1b appears to have an atmosphere most like that of other brown dwarfs. This suggests that surface gravity is very important in controlling the atmospheric systems of substellar mass bodies.

  18. Sustained High Protein-tyrosine Phosphatase 1B Activity in the Sperm of Obese Males Impairs the Sperm Acrosome Reaction*

    PubMed Central

    Shi, Lei; Zhang, Qipeng; Xu, Binqiang; Jiang, Xiaohong; Dai, Yutian; Zhang, Chen-Yu; Zen, Ke

    2014-01-01

    Evidence of a causal link between male obesity and subfertility or infertility has been demonstrated previously. However, the mechanism underlying this link is incompletely understood. Here, we report that sustained high protein-tyrosine phosphatase 1B (PTP1B) activity in sperm of obese donors plays an essential role in coupling male obesity and subfertility or infertility. First, PTP1B level and activity were significantly higher in sperm from ob/ob mice than in wild-type littermates. High PTP1B level and activity in sperm was also observed in obese patients compared with non-obese donors. The enhanced sperm PTP1B level and activity in ob/ob mice and obese patients correlated with a defect of the sperm acrosome reaction (AR). Second, treating sperm from male ob/ob mice or obese men with a specific PTP1B inhibitor largely restored the sperm AR. Finally, blockade of sperm AR by enhanced PTP1B activity in male ob/ob mice or obese men was due to prolonged dephosphorylation of N-ethylmaleimide-sensitive factor by PTP1B, leading to the inability to reassemble the trans-SNARE complexes, which is a critical step in sperm acrosomal exocytosis. In summary, our study demonstrates for the first time that a sustained high PTP1B level or activity in the sperm of obese donors causes a defect of sperm AR and that PTP1B is a novel potential therapeutic target for male infertility treatment. PMID:24519936

  19. Solar energy system performance evaluation: Seasonal report for IBM System 1B, Carlsbad, New Mexico

    NASA Technical Reports Server (NTRS)

    1980-01-01

    A hot solar heating and hot water system's operational performance from April 1979 through March 1980 is evaluated. The space heating and hot water loads were near expected values for the year. Solar energy provided 43 percent of the space heating and 53 percent of the hot water energy. The system did not meet the total system solar fraction design value of 69 percent because of a combination of higher estimated space heating load than was actually encountered and the apportioning of solar energy between the space heating and the domestic hot water loads. System losses and high building temperatures also contributed to this deviation. Total net savings were 23.072 million BTUs. Most of the energy savings came during the winter months, but hot water savings were sufficient to justify running the system during the summer months.

  20. Determination of 7-piperidino-1,2,3,5-tetrahydroimidazo[2,1-b]quinazolin-2-one (DN-9693) in human plasma and urine by high-performance liquid chromatography with electrochemical detection: application to studies on disposition of DN-9693 in healthy human volunteers.

    PubMed

    Tanaka, M; Yamada, M; Ono, K; Takegoshi, T

    1987-03-01

    A procedure for the determination of a newly developed antiadhesive agent, 7-piperidino-1,2,3,5-tetrahydroimidazo[2,1-b]quinazolin-2-on e (DN-9693; 1), in human plasma and urine at low nanogram levels by high-performance liquid chromatography with electrochemical detection is described. Plasma and urine samples were made alkaline with 1 M KOH solution and extracted with chloroform. After removing organic solvent, the extracts reconstituted in methanol were applied to a reversed-phase octadesyl-packed column and eluted with a methanol: phosphate buffer (pH 8.0) mobile phase. The applied potentials were set at 0.80 and 0.95 V versus a silver-silver chloride reference electrode for the analyses of plasma and urine specimens, respectively. The method provides a recovery of greater than 90% and a within-day precision of less than 5%. The minimum quantifiable levels for 1 in plasma and urine using 1 mL of sample are 1 and 4 ng/mL, respectively. The method has been successfully used for the determination of 1 in plasma and urine samples from healthy human volunteers after intravenous infusion of the drug.

  1. Cellular coexistence of two high molecular subsets of eEF1B complex.

    PubMed

    Le Sourd, Frédéric; Cormier, Patrick; Bach, Stéphane; Boulben, Sandrine; Bellé, Robert; Mulner-Lorillon, Odile

    2006-05-15

    The elongation factor eEF1B involved in protein translation was found to contain two isoforms of the eEF1Bdelta subunit in sea urchin eggs. The eEF1Bdelta2 isoform differs from eEF1Bdelta1 by a specific insert of 26 amino acids. Both isoforms are co-expressed in the cell and likely originate from a unique gene. The feature appears universal in metazoans as judged from in silico analysis in EST-databanks. The eEF1B components were co-immunoprecipitated by specific eEF1Bdelta2 antibodies. Quantification of the proteins in immunoprecipitates and on immunoblots demonstrates that eEF1Bdelta1 and eEF1Bdelta2 proteins are present in two subsets of eEF1B complex. We discuss and propose a model for the different subsets of eEF1B complex concomitantly present in the cell.

  2. Project FIRES [Firefighters' Integrated Response Equipment System]. Volume 2: Protective Ensemble Performance Standards, Phase 1B

    NASA Technical Reports Server (NTRS)

    Abeles, F. J.

    1980-01-01

    The design of the prototype protective ensemble was finalized. Prototype ensembles were fabricated and then subjected to a series of qualification tests which were based upon the protective ensemble performance standards PEPS requirements. Engineering drawings and purchase specifications were prepared for the new protective ensemble.

  3. Ionic-liquid-based dispersive liquid-liquid microextraction combined with magnetic solid-phase extraction for the determination of aflatoxins B1 , B2 , G1 , and G2 in animal feeds by high-performance liquid chromatography with fluorescence detection.

    PubMed

    Zhao, Jiao; Zhu, Yan; Jiao, Yang; Ning, Jinyan; Yang, Yaling

    2016-10-01

    A novel two-step extraction technique combining ionic-liquid-based dispersive liquid-liquid microextraction with magnetic solid-phase extraction was developed for the preconcentration and separation of aflatoxins in animal feedstuffs before high-performance liquid chromatography coupled with fluorescence detection. In this work, ionic liquid 1-octyl-3-methylimidazolium hexafluorophosphate was used as the extractant in dispersive liquid-liquid microextraction, and hydrophobic pelargonic acid modified Fe3 O4 magnetic nanoparticles as an efficient adsorbent were applied to retrieve the aflatoxins-containing ionic liquid. Notably, the target of magnetic nanoparticles was the ionic liquid rather than the aflatoxins. Because of the rapid mass transfer associated with the dispersive liquid-liquid microextraction and magnetic solid phase steps, fast extraction could be achieved. The main parameters affecting the extraction recoveries of aflatoxins were investigated and optimized. Under the optimum conditions, vortexing at 2500 rpm for 1 min in the dispersive liquid-liquid microextraction and magnetic solid-phase extraction and then desorption by sonication for 2 min with acetonitrile as eluent. The recoveries were 90.3-103.7% with relative standard deviations of 3.2-6.4%. Good linearity was observed with correlation coefficients ranged from 0.9986 to 0.9995. The detection limits were 0.632, 0.087, 0.422 and 0.146 ng/mL for aflatoxins B1 , B2, G1, and G2, respectively. The results were also compared with the pretreatment method carried out by conventional immunoaffinity columns.

  4. Bioassay-guided isolation of an active compound with protein tyrosine phosphatase 1B inhibitory activity from Sargassum fusiforme by high-speed counter-current chromatography.

    PubMed

    Wang, Miao; Gu, Dongyu; Guo, Xinfeng; Li, Haoquan; Wang, Yi; Guo, Hong; Yang, Yi; Tian, Jing

    2016-11-01

    A rapid and efficient method using high-speed counter-current chromatography was established for the bioassay-guided separation of an active compound with protein tyrosine phosphatase 1B inhibitory activity from Sargassum fusiforme. Under the bioassay guidance, the ethyl acetate extract with the best IC50 value of 0.37 ± 0.07 μg/mL exhibited a potential protein tyrosine phosphatase 1B inhibitory activity, which was further separated by high-speed counter-current chromatography. The separation was performed with a two-phase solvent system composed of n-hexane/methanol/water (5:4:1, v/v). As a result, dibutyl phthalate (19.7 mg) with the purity of 95.3% was obtained from 200 mg of the ethyl acetate extract. Its IC50 was 14.05 ± 0.06 μM, which was further explained by molecular docking. The result of molecular docking showed that dibutyl phthalate enfolded in the catalytic site of protein tyrosine phosphatase 1B. The main force between dibutyl phthalate and protein tyrosine phosphatase 1B was the hydrogen bond interaction with Gln266. In addition, hydrogen bond, van der Waals force and hydrophobic interaction with the amino acids (Ala217, Ile219, and Gly220) were also responsible for the stable protein-ligand complex.

  5. High expression of AKR1B10 predicts low risk of early tumor recurrence in patients with hepatitis B virus-related hepatocellular carcinoma

    PubMed Central

    Wang, Yan-Yan; Qi, Lu-Nan; Zhong, Jian-Hong; Qin, Hong-Gui; Ye, Jia-Zhou; Lu, Shi-Dong; Ma, Liang; Xiang, Bang-De; Li, Le-Qun; You, Xue-Mei

    2017-01-01

    To clarify the relationship between aldo-keto reductase family 1 member B10 (AKR1B10) expression and early hepatocellular carcinoma (HCC) recurrence, this study detected AKR1B10 expression in tumor and adjacent non-tumor tissues from 110 patients with hepatitis B virus (HBV)-related HCC underwent liver resection and analyzed its correlations with clinicopathological characteristics and prognosis of these patients. Detected by quantitative reverse transcription polymerase chain reaction, AKR1B10 mRNA expression showed significantly higher in HCC tissues than in adjacent non-tumor tissues, with a low level in normal liver tissues. Similar results was confirmed at the protein level using immunohistochemistry and Western blotting. High AKR1B10 expression was negatively correlated with serum alpha-fetoprotein level and positively correlated with HBV-DNA level. Patients with high AKR1B10 expression had significantly higher disease-free survival than those with low expression within 2 years after liver resection. Multivariate analysis also confirmed high AKR1B10 expression to be a predictor of low risk of early HCC recurrence. In addition, high AKR1B10 expression was found to be a favorable factor of overall survival. These results suggest that AKR1B10 is involved in HBV-related hepatocarcinogenesis, but its high expression could predict low risk of early tumor recurrence in patients with HBV-related HCC after liver resection. PMID:28181486

  6. Performance, Performance System, and High Performance System

    ERIC Educational Resources Information Center

    Jang, Hwan Young

    2009-01-01

    This article proposes needed transitions in the field of human performance technology. The following three transitions are discussed: transitioning from training to performance, transitioning from performance to performance system, and transitioning from learning organization to high performance system. A proposed framework that comprises…

  7. Molecular and functional characterization of protein 4.1B, a novel member of the protein 4.1 family with high level, focal expression in brain.

    PubMed

    Parra, M; Gascard, P; Walensky, L D; Gimm, J A; Blackshaw, S; Chan, N; Takakuwa, Y; Berger, T; Lee, G; Chasis, J A; Snyder, S H; Mohandas, N; Conboy, J G

    2000-02-04

    Brain-enriched isoforms of skeletal proteins in the spectrin and ankyrin gene families have been described. Here we characterize protein 4.1B, a novel homolog of erythrocyte protein 4.1R that is encoded by a distinct gene. In situ hybridization revealed high level, focal expression of 4.1B mRNA in select neuronal populations within the mouse brain, including Purkinje cells of the cerebellum, pyramidal cells in hippocampal regions CA1-3, thalamic nuclei, and olfactory bulb. Expression was also detected in adrenal gland, kidney, testis, and heart. 4.1B protein exhibits high homology to the membrane binding, spectrin-actin binding, and C-terminal domains of 4.1R, including motifs for interaction with NuMA and FKBP13. cDNA characterization and Western blot analysis revealed multiple spliceoforms of protein 4.1B, with functionally relevant heterogeneity in the spectrin-actin and NuMA binding domains. Regulated alternative splicing events led to expression of unique 4. 1B isoforms in brain and muscle; only the latter possessed a functional spectrin-actin binding domain. By immunofluorescence, 4. 1B was localized specifically at the plasma membrane in regions of cell-cell contact. Together these results indicate that 4.1B transcription is selectively regulated among neuronal populations and that alternative splicing regulates expression of 4.1B isoforms possessing critical functional domains typical of other protein 4.1 family members.

  8. Microstructure and grain refining performance of equal-channel angular-pressed Al-5%Ti-1%B master alloy on pure aluminum

    NASA Astrophysics Data System (ADS)

    Wei, Kun Xia; Liu, Ping; Wei, Wei; Du, Qing Bo; Alexandrov, Igor V.; Hu, Jing

    2016-12-01

    Al-5%Ti-1%B master alloy was subjected to equal-channel angular pressing (ECAP) by route A at room temperature. The effect of the ECAP on the size and the distribution of Al3Ti and TiB2 particles, the fading resistance of the Al-5%Ti-1%B master alloy and the grain refining performance of pure Al ingots with the addition of the Al-5%Ti-1%B master alloy before and after ECAP have been investigated. The large platelet Al3Ti particles were fragmented into fine blocky Al3Ti particles from 88 to 25 μm after eight ECAP passes, and the TiB2 particles were well dispersed in the Al matrix. It has been revealed that grain refining efficiency was improved by adding the Al-5%Ti-1%B master alloy after ECAP to the Al melt. The mean grain size of α-Al was decreased from 1220 to 70 μm with increasing the number of ECAP passes. It has been proved that the grain size of α-Al could be well fitted by the length of Al3Ti particles and the growth restrict factor. Al-5%Ti-1%B master alloy after four ECAP passes appeared to have a better fading resistance due to fine blocky Al3Ti particles.

  9. Development of a novel high-throughput assay for the investigation of GlyT-1b neurotransmitter transporter function.

    PubMed

    Allan, Lynda; Leith, J Lianne; Papakosta, Marianthi; Morrow, John A; Irving, Nicholas G; McFerran, Brian W; Clark, Alan G

    2006-01-01

    The glycine transporter (GlyT-1b) is a Na(+)/Cl(-)-dependent electrogenic transporter which mediates the rapid re-uptake of glycine from the synaptic cleft. Based on its tissue distribution, GlyT-1 has been suggested to co-localise with the NMDA receptor where it may modulate the concentration of glycine at its co-agonist binding site. This data has led to GlyT-1 inhibitors being proposed as targets for disorders such as schizophrenia and cognitive dysfunction. Radiolabelled uptake assays (e.g. [(3)H]glycine) have been traditionally used in compound screening to identify glycine transporter inhibitors. While such an assay format is useful for testing limited numbers of compounds, the identification of novel glycine uptake inhibitors requires a functional assay compatible with high-throughput screening (HTS) of large compound libraries. Here, the authors present the development of a novel homogenous cell-based assay using the FLIPR membrane potential blue dye (Molecular Devices) and FLEXstation. Pharmacological data for the GlyT-1 inhibitors Org 24598 and ALX 5407 obtained using this novel electrogenic assay correlated well with the conventional [(3)H]-glycine uptake assay format. Furthermore, the assay has been successfully miniaturised using FLIPR(3) and therefore has the potential to be used for high-throughput screening.

  10. High agonist-independent activity is a distinguishing feature of the dopamine D1B receptor subtype.

    PubMed

    Tiberi, M; Caron, M G

    1994-11-11

    Dopamine D1A and D1B receptor subtypes belong to the superfamily of G protein-coupled receptors. Both receptors are coupled to the activation of adenylyl cyclase and exhibit distinct brain distribution. To identify functional differences, binding and stimulation of adenylyl cyclase were assessed in 293 cells expressing transiently either dopamine D1A or D1B receptors. Membranes expressing D1B receptors displayed higher affinities for agonists than those expressing D1A receptors, whereas antagonist affinities were lower at the D1B than at the D1A receptor. Basal activity of adenylyl cyclase in whole 293 cells expressing various levels of D1B receptors was significantly higher than the basal activity measured in cells expressing D1A receptors. Maximal activation of adenylyl cyclase resulting from stimulation of the D1B receptor was less than that obtained following agonist activation of the D1A receptor. In cells expressing D1B receptors, agonists displayed an increased potency for stimulating adenylyl cyclase in comparison with the potencies determined for the D1A receptor. On the other hand, certain antagonists displayed "negative efficacy" at both receptor subtypes but had a more profound inhibition on the agonist-independent signaling activity of the D1B receptor. The properties described here are reminiscent of those of constitutively active G protein-coupled receptors obtained by site-directed mutations. Thus, the D1B receptor may represent a naturally occurring receptor subtype with properties akin to those of constitutively active G protein-coupled receptors. The different anatomical distribution and biochemical properties of these D1 receptors strengthen the functional distinctions between the two subtypes and could account for the basis of heterogeneity within a given class of neurotransmitter or hormone receptors. In addition, if these properties are recapitulated in cells expressing the D1B receptors, they may underlie important role in the regulation of

  11. High prevalence of hepatitis C virus genotype 1b infection in a small town of Argentina. Phylogenetic and Bayesian coalescent analysis.

    PubMed

    Golemba, Marcelo D; Di Lello, Federico A; Bessone, Fernando; Fay, Fabian; Benetti, Silvina; Jones, Leandro R; Campos, Rodolfo H

    2010-01-18

    Previous studies in Argentina have documented a general prevalence of Hepatitis C Virus (HCV) infection close to 2%. In addition, a high prevalence of HCV has been recently reported in different Argentinean small rural communities. In this work, we performed a study aimed at analyzing the origins and diversification patterns of an HCV outbreak in Wheelwright, a small rural town located in Santa Fe province (Argentina).A total of 89 out of 1814 blood samples collected from people living in Wheelwright, were positive for HCV infection. The highest prevalence (4.9%) was observed in people older than 50 years, with the highest level for the group aged between 70-79 years (22%). The RFLP analyses showed that 91% of the positive samples belonged to the HCV-1b genotype. The E1/E2 and NS5B genes were sequenced, and their phylogenetic analysis showed that the HCV-1b sequences from Wheelwright were monophyletic. Bayesian coalescent-based methods were used to estimate substitution rates and time of the most recent common ancestor (tMRCA). The mean estimated substitution rates and the tMRCA for E1/E2 with and without HVR1 and NS5B were 7.41E-03 s/s/y and 61 years, 5.05E-03 s/s/y and 58 years and 3.24E-03 s/s/y and 53 years, respectively. In summary, the tMRCA values, the demographic model with constant population size, and the fact that the highest prevalence of infection was observed in elder people support the hypothesis that the HCV-1b introduction in Wheelwright initially occurred at least five decades ago and that the early epidemic was characterized by a fast rate of virus transmission. The epidemic seems to have been controlled later on down to the standard transmission rates observed elsewhere.

  12. High Prevalence of Hepatitis C Virus Genotype 1b Infection in a Small Town of Argentina. Phylogenetic and Bayesian Coalescent Analysis

    PubMed Central

    Golemba, Marcelo D.; Di Lello, Federico A.; Bessone, Fernando; Fay, Fabian; Benetti, Silvina; Jones, Leandro R.; Campos, Rodolfo H.

    2010-01-01

    Previous studies in Argentina have documented a general prevalence of Hepatitis C Virus (HCV) infection close to 2%. In addition, a high prevalence of HCV has been recently reported in different Argentinean small rural communities. In this work, we performed a study aimed at analyzing the origins and diversification patterns of an HCV outbreak in Wheelwright, a small rural town located in Santa Fe province (Argentina). A total of 89 out of 1814 blood samples collected from people living in Wheelwright, were positive for HCV infection. The highest prevalence (4.9%) was observed in people older than 50 years, with the highest level for the group aged between 70–79 years (22%). The RFLP analyses showed that 91% of the positive samples belonged to the HCV-1b genotype. The E1/E2 and NS5B genes were sequenced, and their phylogenetic analysis showed that the HCV-1b sequences from Wheelwright were monophyletic. Bayesian coalescent-based methods were used to estimate substitution rates and time of the most recent common ancestor (tMRCA). The mean estimated substitution rates and the tMRCA for E1/E2 with and without HVR1 and NS5B were 7.41E-03 s/s/y and 61 years, 5.05E-03 s/s/y and 58 years and 3.24E-03 s/s/y and 53 years, respectively. In summary, the tMRCA values, the demographic model with constant population size, and the fact that the highest prevalence of infection was observed in elder people support the hypothesis that the HCV-1b introduction in Wheelwright initially occurred at least five decades ago and that the early epidemic was characterized by a fast rate of virus transmission. The epidemic seems to have been controlled later on down to the standard transmission rates observed elsewhere. PMID:20090919

  13. Triton 2 (1B)

    NASA Technical Reports Server (NTRS)

    Clark, Michelle L.; Meiss, A. G.; Neher, Jason R.; Rudolph, Richard H.

    1994-01-01

    The goal of this project was to perform a detailed design analysis on a conceptually designed preliminary flight trainer. The Triton 2 (1B) must meet the current regulations in FAR Part 23. The detailed design process included the tasks of sizing load carrying members, pulleys, bolts, rivets, and fuselage skin for the safety cage, empennage, and control systems. In addition to the regulations in FAR Part 23, the detail design had to meet established minimums for environmental operating conditions and material corrosion resistance.

  14. The T4 Phage SF1B Helicase Dda Is Structurally Optimized to Perform DNA Strand Separation

    SciTech Connect

    He, Xiaoping; Byrd, Alicia K.; Yun, Mi-Kyung; Pemble IV, Charles W.; Harrison, David; Yeruva, Laxmi; Dahl, Christopher; Kreuzer, Kenneth N.; Raney, Kevin D.; White, Stephen W.

    2012-10-16

    Helicases move on DNA via an ATP binding and hydrolysis mechanism coordinated by well-characterized helicase motifs. However, the translocation along single-stranded DNA (ssDNA) and the strand separation of double-stranded (dsDNA) may be loosely or tightly coupled. Dda is a phage T4 SF1B helicase with sequence homology to the Pif1 family of helicases that tightly couples translocation to strand separation. The crystal structure of the Dda-ssDNA binary complex reveals a domain referred to as the pin that was previously thought to remain static during strand separation. The pin contains a conserved phenylalanine that mediates a transient base-stacking interaction that is absolutely required for separation of dsDNA. The pin is secured at its tip by protein-protein interactions through an extended SH3 domain thereby creating a rigid strut. The conserved interface between the pin and the SH3 domain provides the mechanism for tight coupling of translocation to strand separation.

  15. Production and Screening of High Yield Avermectin B1b Mutant of Streptomyces avermitilis 41445 Through Mutagenesis

    PubMed Central

    Siddique, Samia; Syed, Quratulain; Adnan, Ahmad; Qureshi, Fahim Ashraf

    2014-01-01

    Background: Secondary metabolite production from wild strains is very low for economical purpose therefore certain strain improvement strategies are required to achieve hundred times greater yield of metabolites. Most important strain improvement techniques include physical and chemical mutagenesis. Broad spectrum mutagenesis through UV irradiation is the most important and convenient physical method. Objectives: The present study was conducted for enhanced production of avermectin B1b from Streptomyces avermitilis 41445 by mutagenesis using ultraviolet (UV) radiation, ethidium bromide (EB), and ethyl methanesulfonate (EMS) as mutagens. Materials and Methods: S. avermitilis DSM 41445 maintained on yeast extract malt extract glucose medium (YMG) was used as inoculum for SM2 fermentation medium. Spores of S. avermitilis DSM 41445 were exposed to UV radiation for physical broad spectrum mutagenesis and to EMS and EB for chemical mutagenesis. For each mutagen, the lethality rate and mutation rate were calculated along with positive mutation rate. Results: Avermectin B1b-hyper-producing mutant, produced using these three different methods, was selected according to the HPLC results. The mutant obtained after 45 minutes of UV radiation to the spores of S. avermitilis 41445, was found to be the best mutant for the enhanced production of avermectin B1b component (254.14 mg/L). Other avermectin B1b-hyper-producing mutants, were obtained from EMS (1 µL/mL) and EB (30 µL/mL) treatments, and yielded 202.63 mg/L and 199.30 mg/L of B1b, respectively. Conclusions: The hereditary stability analysis of the UV mentioning 45 minutes revealed the UV exposure time for mutants and 3 represented the colony taken from the plate irradiated for 45 minutes mutant showed that the production of avermectin B1b remained constant and no reverse mutation occurred after 15 generations. PMID:25147669

  16. Deletion of protein tyrosine phosphatase 1B rescues against myocardial anomalies in high fat diet-induced obesity: Role of AMPK-dependent autophagy.

    PubMed

    Kandadi, Machender R; Panzhinskiy, Evgeniy; Roe, Nathan D; Nair, Sreejayan; Hu, Dahai; Sun, Aijun

    2015-02-01

    Obesity-induced cardiomyopathy may be mediated by alterations in multiple signaling cascades involved in glucose and lipid metabolism. Protein tyrosine phosphatase-1B (PTP1B) is an important negative regulator of insulin signaling. This study was designed to evaluate the role of PTP1B in high fat diet-induced cardiac contractile anomalies. Wild-type and PTP1B knockout mice were fed normal (10%) or high (45%) fat diet for 5months prior to evaluation of cardiac function. Myocardial function was assessed using echocardiography and an Ion-Optix MyoCam system. Western blot analysis was employed to evaluate levels of AMPK, mTOR, raptor, Beclin-1, p62 and LC3-II. RT-PCR technique was employed to assess genes involved in hypertrophy and lipid metabolism. Our data revealed increased LV thickness and LV chamber size as well as decreased fractional shortening following high fat diet intake, the effect was nullified by PTP1B knockout. High fat diet intake compromised cardiomyocyte contractile function as evidenced by decreased peak shortening, maximal velocity of shortening/relengthening, intracellular Ca²⁺ release as well as prolonged duration of relengthening and intracellular Ca²⁺ decay, the effects of which were alleviated by PTP1B knockout. High fat diet resulted in enlarged cardiomyocyte area and increased lipid accumulation, which were attenuated by PTP1B knockout. High fat diet intake dampened myocardial autophagy as evidenced by decreased LC3-II conversion and Beclin-1, increased p62 levels as well as decreased phosphorylation of AMPK and raptor, the effects of which were significantly alleviated by PTP1B knockout. Pharmacological inhibition of AMPK using compound C disengaged PTP1B knockout-conferred protection against fatty acid-induced cardiomyocyte contractile anomalies. Taken together, our results suggest that PTP1B knockout offers cardioprotection against high fat diet intake through activation of AMPK. This article is part of a Special Issue entitled

  17. Anti-obesity and hypolipidemic effects of Rheum undulatum in high-fat diet-fed C57BL/6 mice through protein tyrosine phosphatase 1B inhibition.

    PubMed

    Lee, Woojung; Yoon, Goo; Hwang, Ye Ran; Kim, Yong Kee; Kim, Su-Nam

    2012-03-01

    Protein tyrosine phosphatase 1B (PTP1B) is important in the regulation of metabolic diseases and has emerged as a promising signaling target. Previously, we reported the PTP1B inhibitory activity of Rheum undulatum (RU). In the present study, we investigated the metabolic regulatory effects of RU in a high-fat diet (HFD) model. RU treatment significantly blocked body weight gain, which was accompanied by a reduction of feed efficiency. In addition, it led to a reduction of liver weight mediated by overexpression of PPARα and CPT1 in the liver, and an increase in the expression of adiponectin, aP2, and UCP3 in adipose tissue responsible for the reduction of total and LDL-cholesterol levels. Chrysophanol and physcion from RU significantly inhibited PTP1B activity and strongly enhanced insulin sensitivity. Altogether, our findings strongly suggest that 2 compounds are novel PTP1B inhibitors and might be considered as anti-obesity agents that are effective for suppressing body weight gain and improving lipid homeostasis.

  18. High performance polymer development

    NASA Technical Reports Server (NTRS)

    Hergenrother, Paul M.

    1991-01-01

    The term high performance as applied to polymers is generally associated with polymers that operate at high temperatures. High performance is used to describe polymers that perform at temperatures of 177 C or higher. In addition to temperature, other factors obviously influence the performance of polymers such as thermal cycling, stress level, and environmental effects. Some recent developments at NASA Langley in polyimides, poly(arylene ethers), and acetylenic terminated materials are discussed. The high performance/high temperature polymers discussed are representative of the type of work underway at NASA Langley Research Center. Further improvement in these materials as well as the development of new polymers will provide technology to help meet NASA future needs in high performance/high temperature applications. In addition, because of the combination of properties offered by many of these polymers, they should find use in many other applications.

  19. High-resolution melt PCR analysis for rapid identification of Chlamydia abortus live vaccine strain 1B among C. abortus strains and field isolates.

    PubMed

    Vorimore, Fabien; Cavanna, Noémie; Vicari, Nadia; Magnino, Simone; Willems, Hermann; Rodolakis, Annie; Siarkou, Victoria I; Laroucau, Karine

    2012-09-01

    We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus live vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.

  20. TES Level 1B

    Atmospheric Science Data Center

    2014-12-08

    TES Level 1B data files contain radiometric calibrated spectral radiances and their ... and some engineering data are also provided. A Level 1B data file contains data from a single TES orbit for one focal ... as the Aura orbit number at the time of the South Pole apex crossing. version id represents the version identification number, ...

  1. A new high-resolution model of non-tidal atmosphere and ocean mass variability for de-aliasing of satellite gravity observations: AOD1B RL06

    NASA Astrophysics Data System (ADS)

    Dobslaw, H.; Bergmann-Wolf, I.; Dill, R.; Poropat, L.; Thomas, M.; Dahle, C.; Esselborn, S.; König, R.; Flechtner, F.

    2017-10-01

    The release 06 (RL06) of the Gravity Recovery and Climate Experiment (GRACE) Atmosphere and Ocean De-Aliasing Level-1B (AOD1B) product has been prepared for use as a time-variable background model in global gravity research. Available since the year 1976 with a temporal resolution of 3 hr, the product is provided in Stokes coefficients up to degree and order 180. RL06 separates tidal and non-tidal signals, and has an improved long-term consistency due to the introduction of a time-invariant reference orography in continental regions. Variance reduction tests performed with globally distributed in situ ocean bottom pressure recordings and sea-surface height anomalies from Jason-2 over a range of different frequency bands indicate a generally improved performance of RL06 compared to its predecessor. Orbit tests for two altimetry satellites remain inconclusive, but GRACE K-band residuals are reduced by 0.031 nm s-2 in a global average, and by more than 0.5 nm s-2 at numerous places along the Siberian shelf when applying the latest AOD1B release. We therefore recommend AOD1B RL06 for any upcoming satellite gravimetry reprocessing effort.

  2. Effect of timing of challenge following short-term natural exposure to bovine viral diarrhea virus type 1b on animal performance and immune response in beef steers.

    PubMed

    Carlos-Valdez, L; Wilson, B K; Burciaga-Robles, L O; Step, D L; Holland, B P; Richards, C J; Montelongo, M A; Confer, A W; Fulton, R W; Krehbiel, C R

    2016-11-01

    Bovine respiratory disease (BRD) is the most common and economically detrimental disease of beef cattle during the postweaning period, causing the majority of morbidity and mortality in feedlots. The pathogenesis of this disease often includes an initial viral infection, which can predispose cattle to a secondary bacterial infection. The objective of this experiment was to determine the effects of timing of an intratracheal (MH) challenge relative to 72 h of natural exposure to bovine viral diarrhea virus (BVDV) type 1b persistently infected (PI) calves on performance, serum antibody production, total and differential white blood cell (WBC) count, rectal temperature, clinical severity score (CS), and haptoglobin (Hp). Steers ( = 24; 276 ± 31 kg initial BW) were randomly allocated to 1 of 3 treatments (8 steers/treatment) in a randomized complete block design. Treatments were steers not exposed to calves PI with BVDV 1b and not challenged with MH (CON), steers intratracheally challenged with MH 84 h after being exposed to calves PI with BVDV 1b for 72 h (LateCh), and steers intratracheally challenged with MH 12 h after being exposed to calves PI with BVDV 1b for 72 h (EarlyCh). Performance (ADG, DMI, and G:F) was decreased ( < 0.001) for both EarlyCh and LateCh from d 0 to 4. From d 5 to 17, LateCh appeared to compensate for this lost performance and demonstrated increased ADG ( = 0.01) and G:F ( = 0.01) compared with EarlyCh. Both EarlyCh and LateCh had decreased platelet counts ( < 0.001) compared with CON. Antibody concentrations of BVDV and MH were higher ( < 0.05) for both EarlyCh and LateCh compared with CON. Rectal temperature, CS, and Hp increased ( < 0.001) across time from h 4 to 48, h 4 to 36, and h 8 to 168, respectively. Within 24 h of MH challenge, WBC and neutrophil concentrations within the blood increased whereas lymphocyte concentrations decreased. The timing of BVDV exposure relative to a MH challenge appears to influence the CS and acute phase

  3. Environmental Performance Report 2013: Annual Site Environmental Report per the U.S. Department of Energy Order 231.1-1B (Management Publication)

    SciTech Connect

    Schlomberg, K.; Eickhoff, J.; Beatty, B.; Braus, G.; Durbin, L.; Fiehweg, R.; Ray, M.; Ryon, T.; Schmitz, E.

    2014-08-01

    The National Renewable Energy Laboratory's (NREL's) Environmental Performance Report provides a description of the laboratory's environmental management activities for 2013, including information on environmental and sustainability performance, environmental compliance activities and status, and environmental protection programs, highlights, and successes. The purpose of this report is to ensure that U.S. Department of Energy (DOE) and the public receive timely, accurate information about events that have affected or could adversely affect the health, safety, and security of the public or workers; the environment; or the operations of DOE facilities. This report meets the requirements of the Annual Site Environmental Report and is prepared in accordance with the DOE Order 231.1B, Environment, Safety and Health Reporting.

  4. The mechanism and effect of defects in the B1 B2 phase transition of KCl under high pressure: molecular dynamics simulation

    NASA Astrophysics Data System (ADS)

    Kinoshita, Takahiro; Mashimo, Tsutomu; Kawamura, Katsuyuki

    2005-02-01

    Molecular dynamics (MD) simulations of the pressure-induced phase transition of potassium chloride (KCl) were performed with 2744 and 9000 atoms to study crystalline states, the process and the effects of defects under high hydrostatic pressure. In this study, we adopted the Born-Mayer-Huggins-type potential function to describe the interatomic interaction. The potential parameters used in this study were empirically optimized on the basis of Hugoniot equation of state data. The simulation results for perfect crystals (1372 K+ and 1372 Cl-) showed that the B1-B2 phase transition occurred with large hysteresis, and the thermodynamic transition pressure was calculated to be 3.5 GPa. The simulation results indicated that the phase transition proceeded through displacements of atomic lines parallel to the \\langle 100\\rangle axis direction of the B1-type structure, and that these lines corresponded to the atomic lines parallel to the \\langle 111\\rangle axis direction of the B2-type structure after the phase transition. In the case of the larger cells containing 9000 atoms with weak or strong van der Waals interactions, some clusters or dislocations, respectively, were generated in the resultant B2 phase. As regards dislocations, the phase transitions started around dislocations and the phase transition pressure decreased.

  5. High performance systems

    SciTech Connect

    Vigil, M.B.

    1995-03-01

    This document provides a written compilation of the presentations and viewgraphs from the 1994 Conference on High Speed Computing given at the High Speed Computing Conference, {open_quotes}High Performance Systems,{close_quotes} held at Gleneden Beach, Oregon, on April 18 through 21, 1994.

  6. Analogs of alpha-melanocyte stimulating hormone with high agonist potency and selectivity at human melanocortin receptor 1b: the role of Trp(9) in molecular recognition.

    PubMed

    Bednarek, Maria A; Macneil, Tanya; Tang, Rui; Fong, Tung M; Angeles Cabello, M; Maroto, Marta; Teran, Ana

    2008-05-01

    alpha-Melanocyte stimulating hormone (alphaMSH), Ac-Ser(1)-Tyr(2)-Ser(3)-Met(4)-Glu(5)-His(6)-Phe(7)-Arg(8)-Trp(9)-Gly(10)-Lys(11)-Pro(12)-Val(13)-NH(2), is an endogenous agonist for the melanocortin receptor 1 (MC1R), the receptor found in the skin, several types of immune cells, and other peripheral sites. Three-dimensional models of complexes of this receptor with alphaMSH and its synthetic analog NDP-alphaMSH, Ac-Ser(1)-Tyr(2)-Ser(3)-Nle(4)-Glu(5)-His(6)-D-Phe(7)-Arg(8)-Trp(9)-Gly(10)-Lys(11)-Pro(12)-Val(13)-NH(2), have been previously proposed. In those models, the 6-9 segment of the ligand was considered essential for the ligand-receptor interactions. In this study, we probed the role of Trp(9) of NDP-alphaMSH in interactions with hMC1bR. Analogs of NDP-alphaMSH with various amino acids in place of Trp(9) were synthesized and tested in vitro in receptor affinity binding and cAMP functional assays at human melanocortin receptors 1b, 3, 4, and 5 (hMC1b,3-5R). Several new compounds displayed high agonist potency at hMC1bR (EC(50) = 0.5-5 nM) and receptor subtype selectivity greater than 2000-fold versus hMC3-5R. The Trp(9) residue of NDP-alphaMSH was determined to be not essential for molecular recognition at hMC1bR.

  7. Human aldo-keto reductases 1B1 and 1B10: a comparative study on their enzyme activity toward electrophilic carbonyl compounds.

    PubMed

    Shen, Yi; Zhong, Linlin; Johnson, Stephen; Cao, Deliang

    2011-05-30

    Aldo-keto reductase family 1 member B1 (AKR1B1, 1B1 in brief) and aldo-keto reductase family 1 member B10 (AKR1B10, 1B10 in brief) are two proteins with high similarities in their amino acid sequences, stereo structures, and substrate specificity. However, these two proteins exhibit distinct tissue distributions; 1B10 is primarily expressed in the gastrointestinal tract and adrenal gland, whereas 1B1 is ubiquitously present in all tissues/organs, suggesting their difference in biological functions. This study evaluated in parallel the enzyme activity of 1B1 and 1B10 toward alpha, beta-unsaturated carbonyl compounds with cellular and dietary origins, including acrolein, crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, and trans-2,4-hexadienal. Our results showed that 1B10 had much better enzyme activity and turnover rates toward these chemicals than 1B1. By detecting the enzymatic products using high-performance liquid chromatography, we measured their activity to carbonyl compounds at low concentrations. Our data showed that 1B10 efficiently reduced the tested carbonyl compounds at physiological levels, but 1B1 was less effective. Ectopically expressed 1B10 in 293T cells effectively eliminated 4-hydroxynonenal at 5 μM by reducing to 1,4-dihydroxynonene, whereas endogenously expressed 1B1 did not. The 1B1 and 1B10 both showed enzyme activity to glutathione-conjugated carbonyl compounds, but 1B1 appeared more active in general. Together our data suggests that 1B10 is more effectual in eliminating free electrophilic carbonyl compounds, but 1B1 seems more important in the further detoxification of glutathione-conjugated carbonyl compounds. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

  8. High Performance Polymers

    NASA Technical Reports Server (NTRS)

    Venumbaka, Sreenivasulu R.; Cassidy, Patrick E.

    2003-01-01

    This report summarizes results from research on high performance polymers. The research areas proposed in this report include: 1) Effort to improve the synthesis and to understand and replicate the dielectric behavior of 6HC17-PEK; 2) Continue preparation and evaluation of flexible, low dielectric silicon- and fluorine- containing polymers with improved toughness; and 3) Synthesis and characterization of high performance polymers containing the spirodilactam moiety.

  9. Overcoming intrinsic multidrug resistance in melanoma by blocking the mitochondrial respiratory chain of slow-cycling JARID1B(high) cells.

    PubMed

    Roesch, Alexander; Vultur, Adina; Bogeski, Ivan; Wang, Huan; Zimmermann, Katharina M; Speicher, David; Körbel, Christina; Laschke, Matthias W; Gimotty, Phyllis A; Philipp, Stephan E; Krause, Elmar; Pätzold, Sylvie; Villanueva, Jessie; Krepler, Clemens; Fukunaga-Kalabis, Mizuho; Hoth, Markus; Bastian, Boris C; Vogt, Thomas; Herlyn, Meenhard

    2013-06-10

    Despite success with BRAFV600E inhibitors, therapeutic responses in patients with metastatic melanoma are short-lived because of the acquisition of drug resistance. We identified a mechanism of intrinsic multidrug resistance based on the survival of a tumor cell subpopulation. Treatment with various drugs, including cisplatin and vemurafenib, uniformly leads to enrichment of slow-cycling, long-term tumor-maintaining melanoma cells expressing the H3K4-demethylase JARID1B/KDM5B/PLU-1. Proteome-profiling revealed an upregulation in enzymes of mitochondrial oxidative-ATP-synthesis (oxidative phosphorylation) in this subpopulation. Inhibition of mitochondrial respiration blocked the emergence of the JARID1B(high) subpopulation and sensitized melanoma cells to therapy, independent of their genotype. Our findings support a two-tiered approach combining anticancer agents that eliminate rapidly proliferating melanoma cells with inhibitors of the drug-resistant slow-cycling subpopulation. Copyright © 2013 Elsevier Inc. All rights reserved.

  10. Cyclic analogs of alpha-melanocyte-stimulating hormone (alphaMSH) with high agonist potency and selectivity at human melanocortin receptor 1b.

    PubMed

    Bednarek, Maria A; MacNeil, Tanya; Tang, Rui; Fong, Tung M; Cabello, M Angeles; Maroto, Marta; Teran, Ana

    2008-06-01

    Alpha-melanotropin (alphaMSH), Ac-Ser1-Tyr2-Ser3-Met4-Glu5-His6-Phe7-Arg8-Trp9-Gly10-Lys11-Pro12-Val13-NH2,(1) has been long recognized as an important physiological regulator of skin and hair pigmentation in mammals. Binding of this peptide to the melanocortin receptor 1 (MC1R) leads to activation of tyrosinase, the key enzyme of the melanin biosynthesis pathway. In this study, interactions of the human MC1bR (an isoform of the receptor 1a) with the synthetic cyclic analogs of alphaMSH were studied. These ligands were analogs of MTII, Ac-Nle4-cyclo-(Asp5-His6-D-Phe7-Arg8-Trp9-Lys10)-NH2, a potent pan-agonist at the human melanocortin receptors (hMC1,3-5R). In the structure of MTII, the His6-D-Phe7-Arg8-Trp9 segment has been recognized as "essential" for molecular recognition at the human melanocortin receptors (hMC1,3-5R). Herein, the role of the Trp9 in the ligand interactions with the hMC1b,3-5R has been reevaluated. Analogs with various amino acids in place of Trp9 were synthesized and tested in vitro in receptor affinity binding and cAMP functional assays at human melanocortin receptors 1b, 3, 4 and 5 (hMC1b,3-5R). Several of the new peptides were high potency agonists (partial) at hMC1bR (EC50 from 0.5 to 20 nM) and largely inactive at hMC3-5R. The bulky aromatic side chain in position 9, such as that in Trp, was found not to be essential to agonism (partial) of the studied peptides at hMC1bR.

  11. High performance polymeric foams

    SciTech Connect

    Gargiulo, M.; Sorrentino, L.; Iannace, S.

    2008-08-28

    The aim of this work was to investigate the foamability of high-performance polymers (polyethersulfone, polyphenylsulfone, polyetherimide and polyethylenenaphtalate). Two different methods have been used to prepare the foam samples: high temperature expansion and two-stage batch process. The effects of processing parameters (saturation time and pressure, foaming temperature) on the densities and microcellular structures of these foams were analyzed by using scanning electron microscopy.

  12. High-Performance Happy

    ERIC Educational Resources Information Center

    O'Hanlon, Charlene

    2007-01-01

    Traditionally, the high-performance computing (HPC) systems used to conduct research at universities have amounted to silos of technology scattered across the campus and falling under the purview of the researchers themselves. This article reports that a growing number of universities are now taking over the management of those systems and…

  13. High-Performance Happy

    ERIC Educational Resources Information Center

    O'Hanlon, Charlene

    2007-01-01

    Traditionally, the high-performance computing (HPC) systems used to conduct research at universities have amounted to silos of technology scattered across the campus and falling under the purview of the researchers themselves. This article reports that a growing number of universities are now taking over the management of those systems and…

  14. High performance parallel architectures

    SciTech Connect

    Anderson, R.E. )

    1989-09-01

    In this paper the author describes current high performance parallel computer architectures. A taxonomy is presented to show computer architecture from the user programmer's point-of-view. The effects of the taxonomy upon the programming model are described. Some current architectures are described with respect to the taxonomy. Finally, some predictions about future systems are presented. 5 refs., 1 fig.

  15. High Performance, Dependable Multiprocessor

    NASA Technical Reports Server (NTRS)

    Ramos, Jeremy; Samson, John R.; Troxel, Ian; Subramaniyan, Rajagopal; Jacobs, Adam; Greco, James; Cieslewski, Grzegorz; Curreri, John; Fischer, Michael; Grobelny, Eric; George, Alan; Aggarwal, Vikas; Patel, Minesh; Some, Raphael

    2006-01-01

    With the ever increasing demand for higher bandwidth and processing capacity of today's space exploration, space science, and defense missions, the ability to efficiently apply commercial-off-the-shelf (COTS) processors for on-board computing is now a critical need. In response to this need, NASA's New Millennium Program office has commissioned the development of Dependable Multiprocessor (DM) technology for use in payload and robotic missions. The Dependable Multiprocessor technology is a COTS-based, power efficient, high performance, highly dependable, fault tolerant cluster computer. To date, Honeywell has successfully demonstrated a TRL4 prototype of the Dependable Multiprocessor [I], and is now working on the development of a TRLS prototype. For the present effort Honeywell has teamed up with the University of Florida's High-performance Computing and Simulation (HCS) Lab, and together the team has demonstrated major elements of the Dependable Multiprocessor TRLS system.

  16. Survey of familial glaucoma shows a high incidence of cytochrome P450, family 1, subfamily B, polypeptide 1 (CYP1B1) mutations in non-consanguineous congenital forms in a Spanish population

    PubMed Central

    Millá, Elena; Mañé, Begoña; Duch, Susana; Hernan, Imma; Borràs, Emma; Planas, Ester; Dias, Miguel de Sousa; Carballo, Miguel

    2013-01-01

    Purpose To identify myocilin (MYOC) and cytochrome P450, family 1, subfamily B, polypeptide 1 (CYP1B1) mutations in a Spanish population with different clinical forms of familial glaucoma or ocular hypertension (OHT). Methods Index patients from 226 families participated in this study. Patients were diagnosed with familial glaucoma or OHT by complete ophthalmologic examination. Screening for MYOC mutations was performed in 207 index patients: 96 with adult-onset primary open-angle glaucoma (POAG), 21 with primary congenital glaucoma (PCG), 18 with juvenile-onset open-angle glaucoma (JOAG), five with Axenfeld-Rieger syndrome (ARS), and 67 with other types of glaucoma. One hundred two of the families (including all those in whom a MYOC mutation was detected) were also screened for CYP1B1 mutations: 45 POAG, 25 PCG, 21 JOAG, four ARS, and seven others. Results We examined 292 individuals (patients and relatives) with a positive family history of glaucoma or OHT. We identified two novel MYOC variants, p.Lys39Arg and p.Glu218Lys, in two families with POAG, and six previously reported MYOC mutations in seven families with POAG (four), JOAG (one), PCG (one), and normotensive glaucoma (one). CYP1B1 mutations were found in 16 index patients with PCG (nine), POAG (three), JOAG (two), and ARS (two). Conclusions The high percentage (9/25=36%) of mutations in CYP1B1 found in non-consanguineous patients with congenital glaucoma mandates genetic testing. However, the percentage of mutations (9/207=4.4%) in MYOC associated with glaucoma is relatively low in our population. The variable phenotype expression of glaucoma, even in families, cannot be explained with a digenic mechanism between MYOC and CYP1B1. PMID:23922489

  17. High performance steam development

    SciTech Connect

    Duffy, T.; Schneider, P.

    1995-12-31

    DOE has launched a program to make a step change in power plant to 1500 F steam, since the highest possible performance gains can be achieved in a 1500 F steam system when using a topping turbine in a back pressure steam turbine for cogeneration. A 500-hour proof-of-concept steam generator test module was designed, fabricated, and successfully tested. It has four once-through steam generator circuits. The complete HPSS (high performance steam system) was tested above 1500 F and 1500 psig for over 102 hours at full power.

  18. High Performance FORTRAN

    NASA Technical Reports Server (NTRS)

    Mehrotra, Piyush

    1994-01-01

    High performance FORTRAN is a set of extensions for FORTRAN 90 designed to allow specification of data parallel algorithms. The programmer annotates the program with distribution directives to specify the desired layout of data. The underlying programming model provides a global name space and a single thread of control. Explicitly parallel constructs allow the expression of fairly controlled forms of parallelism in particular data parallelism. Thus the code is specified in a high level portable manner with no explicit tasking or communication statements. The goal is to allow architecture specific compilers to generate efficient code for a wide variety of architectures including SIMD, MIMD shared and distributed memory machines.

  19. High Performance Window Retrofit

    SciTech Connect

    Shrestha, Som S; Hun, Diana E; Desjarlais, Andre Omer

    2013-12-01

    The US Department of Energy (DOE) Office of Energy Efficiency and Renewable Energy (EERE) and Traco partnered to develop high-performance windows for commercial building that are cost-effective. The main performance requirement for these windows was that they needed to have an R-value of at least 5 ft2 F h/Btu. This project seeks to quantify the potential energy savings from installing these windows in commercial buildings that are at least 20 years old. To this end, we are conducting evaluations at a two-story test facility that is representative of a commercial building from the 1980s, and are gathering measurements on the performance of its windows before and after double-pane, clear-glazed units are upgraded with R5 windows. Additionally, we will use these data to calibrate EnergyPlus models that we will allow us to extrapolate results to other climates. Findings from this project will provide empirical data on the benefits from high-performance windows, which will help promote their adoption in new and existing commercial buildings. This report describes the experimental setup, and includes some of the field and simulation results.

  20. Expression of MAP1B protein and its phosphorylated form MAP1B-P in the CNS of a continuously growing fish, the rainbow trout.

    PubMed

    Alfei, Laura; Soares, Sylvia; Alunni, Alessandro; Ravaille-Veron, Michèle; Von Boxberg, Ysander; Nothias, Fatiha

    2004-05-29

    Microtubule-associated protein-1B (MAP1B), and particularly its phosphorylated isoform MAP1B-P, play an important role in axonal outgrowth during development of the mammalian nervous system and have also been shown to be associated with axonal plasticity in the adult. Here, we used antibodies and mRNA probes directed against mammalian MAP1B to extend our analysis to fish species, trout (Oncorhynchus mykiss), at different stages of development. The specificity of the cross-reaction of our anti-total-MAP1B/MAP1B-P antibodies was confirmed by Western blotting. Trout MAP1B-like proteins exhibited about the same apparent molecular weight (320 kDa) as rat-MAP1B. Immunohistochemistry and in situ hybridization analysis performed on hindbrain and spinal cord revealed the presence of MAP1B in neurons and some glial subpopulations. Primary sensory neurons and motoneurons maintain high levels of MAP1B expression from early stages throughout adulthood, as has been shown for mammals. Unlike mammals, however, MAP1B and axon-specific MAP1B-P continue to be strongly expressed by hindbrain neurons projecting into spinal cord, with the important exception of Mauthner cells. MAP1B/MAP1B-P immunostaining were also detected elsewhere within the brain, including axons of the retino-tectal projection. This obvious difference between adult fish and mammals is likely to reflect the capacity of fish for continued growth and regeneration. Our results suggest that MAP1B/MAP1B-P expression is generally maintained in neurons known to regenerate after axotomy. The regenerative potential of the adult nervous system may in fact depend on continued expression of neuron-intrinsic growth related proteins, a feature of MAP1B that appears phylogenetically conserved.

  1. High Performance Buildings Database

    DOE Data Explorer

    The High Performance Buildings Database is a shared resource for the building industry, a unique central repository of in-depth information and data on high-performance, green building projects across the United States and abroad. The database includes information on the energy use, environmental performance, design process, finances, and other aspects of each project. Members of the design and construction teams are listed, as are sources for additional information. In total, up to twelve screens of detailed information are provided for each project profile. Projects range in size from small single-family homes or tenant fit-outs within buildings to large commercial and institutional buildings and even entire campuses. The database is a data repository as well. A series of Web-based data-entry templates allows anyone to enter information about a building project into the database. Once a project has been submitted, each of the partner organizations can review the entry and choose whether or not to publish that particular project on its own Web site.

  2. High Performance Liquid Chromatography

    NASA Astrophysics Data System (ADS)

    Talcott, Stephen

    High performance liquid chromatography (HPLC) has many applications in food chemistry. Food components that have been analyzed with HPLC include organic acids, vitamins, amino acids, sugars, nitrosamines, certain pesticides, metabolites, fatty acids, aflatoxins, pigments, and certain food additives. Unlike gas chromatography, it is not necessary for the compound being analyzed to be volatile. It is necessary, however, for the compounds to have some solubility in the mobile phase. It is important that the solubilized samples for injection be free from all particulate matter, so centrifugation and filtration are common procedures. Also, solid-phase extraction is used commonly in sample preparation to remove interfering compounds from the sample matrix prior to HPLC analysis.

  3. Myeloid-cell protein tyrosine phosphatase-1B deficiency in mice protects against high-fat diet and lipopolysaccharide-induced inflammation, hyperinsulinemia, and endotoxemia through an IL-10 STAT3-dependent mechanism.

    PubMed

    Grant, Louise; Shearer, Kirsty D; Czopek, Alicja; Lees, Emma K; Owen, Carl; Agouni, Abdelali; Workman, James; Martin-Granados, Cristina; Forrester, John V; Wilson, Heather M; Mody, Nimesh; Delibegovic, Mirela

    2014-02-01

    Protein tyrosine phosphatase-1B (PTP1B) negatively regulates insulin and leptin signaling, rendering it an attractive drug target for treatment of obesity-induced insulin resistance. However, some studies suggest caution when targeting macrophage PTP1B, due to its potential anti-inflammatory role. We assessed the role of macrophage PTP1B in inflammation and whole-body metabolism using myeloid-cell (LysM) PTP1B knockout mice (LysM PTP1B). LysM PTP1B mice were protected against lipopolysaccharide (LPS)-induced endotoxemia and hepatic damage associated with decreased proinflammatory cytokine secretion in vivo. In vitro, LPS-treated LysM PTP1B bone marrow-derived macrophages (BMDMs) displayed increased interleukin (IL)-10 mRNA expression, with a concomitant decrease in TNF-α mRNA levels. These anti-inflammatory effects were associated with increased LPS- and IL-10-induced STAT3 phosphorylation in LysM PTP1B BMDMs. Chronic inflammation induced by high-fat (HF) feeding led to equally beneficial effects of macrophage PTP1B deficiency; LysM PTP1B mice exhibited improved glucose and insulin tolerance, protection against LPS-induced hyperinsulinemia, decreased macrophage infiltration into adipose tissue, and decreased liver damage. HF-fed LysM PTP1B mice had increased basal and LPS-induced IL-10 levels, associated with elevated STAT3 phosphorylation in splenic cells, IL-10 mRNA expression, and expansion of cells expressing myeloid markers. These increased IL-10 levels negatively correlated with circulating insulin and alanine transferase levels. Our studies implicate myeloid PTP1B in negative regulation of STAT3/IL-10-mediated signaling, highlighting its inhibition as a potential anti-inflammatory and antidiabetic target in obesity.

  4. High Performance Parallel Architectures

    NASA Technical Reports Server (NTRS)

    El-Ghazawi, Tarek; Kaewpijit, Sinthop

    1998-01-01

    Traditional remote sensing instruments are multispectral, where observations are collected at a few different spectral bands. Recently, many hyperspectral instruments, that can collect observations at hundreds of bands, have been operational. Furthermore, there have been ongoing research efforts on ultraspectral instruments that can produce observations at thousands of spectral bands. While these remote sensing technology developments hold great promise for new findings in the area of Earth and space science, they present many challenges. These include the need for faster processing of such increased data volumes, and methods for data reduction. Dimension Reduction is a spectral transformation, aimed at concentrating the vital information and discarding redundant data. One such transformation, which is widely used in remote sensing, is the Principal Components Analysis (PCA). This report summarizes our progress on the development of a parallel PCA and its implementation on two Beowulf cluster configuration; one with fast Ethernet switch and the other with a Myrinet interconnection. Details of the implementation and performance results, for typical sets of multispectral and hyperspectral NASA remote sensing data, are presented and analyzed based on the algorithm requirements and the underlying machine configuration. It will be shown that the PCA application is quite challenging and hard to scale on Ethernet-based clusters. However, the measurements also show that a high- performance interconnection network, such as Myrinet, better matches the high communication demand of PCA and can lead to a more efficient PCA execution.

  5. High Performance Parallel Architectures

    NASA Technical Reports Server (NTRS)

    El-Ghazawi, Tarek; Kaewpijit, Sinthop

    1998-01-01

    Traditional remote sensing instruments are multispectral, where observations are collected at a few different spectral bands. Recently, many hyperspectral instruments, that can collect observations at hundreds of bands, have been operational. Furthermore, there have been ongoing research efforts on ultraspectral instruments that can produce observations at thousands of spectral bands. While these remote sensing technology developments hold great promise for new findings in the area of Earth and space science, they present many challenges. These include the need for faster processing of such increased data volumes, and methods for data reduction. Dimension Reduction is a spectral transformation, aimed at concentrating the vital information and discarding redundant data. One such transformation, which is widely used in remote sensing, is the Principal Components Analysis (PCA). This report summarizes our progress on the development of a parallel PCA and its implementation on two Beowulf cluster configuration; one with fast Ethernet switch and the other with a Myrinet interconnection. Details of the implementation and performance results, for typical sets of multispectral and hyperspectral NASA remote sensing data, are presented and analyzed based on the algorithm requirements and the underlying machine configuration. It will be shown that the PCA application is quite challenging and hard to scale on Ethernet-based clusters. However, the measurements also show that a high- performance interconnection network, such as Myrinet, better matches the high communication demand of PCA and can lead to a more efficient PCA execution.

  6. High Performance Work Practices and Firm Performance.

    ERIC Educational Resources Information Center

    Department of Labor, Washington, DC. Office of the American Workplace.

    A literature survey established that a substantial amount of research has been conducted on the relationship between productivity and the following specific high performance work practices: employee involvement in decision making, compensation linked to firm or worker performance, and training. According to these studies, high performance work…

  7. High performance sapphire windows

    NASA Technical Reports Server (NTRS)

    Bates, Stephen C.; Liou, Larry

    1993-01-01

    High-quality, wide-aperture optical access is usually required for the advanced laser diagnostics that can now make a wide variety of non-intrusive measurements of combustion processes. Specially processed and mounted sapphire windows are proposed to provide this optical access to extreme environment. Through surface treatments and proper thermal stress design, single crystal sapphire can be a mechanically equivalent replacement for high strength steel. A prototype sapphire window and mounting system have been developed in a successful NASA SBIR Phase 1 project. A large and reliable increase in sapphire design strength (as much as 10x) has been achieved, and the initial specifications necessary for these gains have been defined. Failure testing of small windows has conclusively demonstrated the increased sapphire strength, indicating that a nearly flawless surface polish is the primary cause of strengthening, while an unusual mounting arrangement also significantly contributes to a larger effective strength. Phase 2 work will complete specification and demonstration of these windows, and will fabricate a set for use at NASA. The enhanced capabilities of these high performance sapphire windows will lead to many diagnostic capabilities not previously possible, as well as new applications for sapphire.

  8. High performing micromachined retroreflector

    NASA Astrophysics Data System (ADS)

    Lundvall, Axel; Nikolajeff, Fredrik; Lindstrom, Tomas

    2003-10-01

    This paper reports on the realization of a type of micromachined retroreflecting sheeting material. The geometry presented has high reflection efficiency even at large incident angles, and it can be manufactured through polymer replication techniques. The paper consists of two parts: A theoretical section outlining the design parameters and their impact on the optical performance, and secondly, an experimental part comprising both manufacturing and optical evaluation for a candidate retroreflecting sheet material in traffic control devices. Experimental data show that the retroreflecting properties are promising. The retroreflector consists of a front layer of densely packed spherical microlenses, a back surface of densely packed spherical micromirrors, and a transparent spacer layer. The thickness of the spacer layer determines in part the optical characteristics of the retroreflector.

  9. ESA Swarm Mission - Level 1b Products

    NASA Astrophysics Data System (ADS)

    Tøffner-Clausen, Lars; Floberghagen, Rune; Mecozzi, Riccardo; Menard, Yvon

    2014-05-01

    Swarm, a three-satellite constellation to study the dynamics of the Earth's magnetic field and its interactions with the Earth system, has been launched in November 2013. The objective of the Swarm mission is to provide the best ever survey of the geomagnetic field and its temporal evolution, which will bring new insights into the Earth system by improving our understanding of the Earth's interior and environment. The Level 1b Products of the Swarm mission contain time-series of the quality screened, calibrated, corrected, and fully geo-localized measurements of the magnetic field intensity, the magnetic field vector (provided in both instrument and Earth-fixed frames), the plasma density, temperature, and velocity. Additionally, quality screened and pre-calibrated measurements of the nongravitational accelerations are provided. Geo-localization is performed by 24- channel GPS receivers and by means of unique, three head Advanced Stellar Compasses for high-precision satellite attitude information. The Swarm Level 1b data will be provided in daily products separately for each of the three Swarm spacecrafts. This poster will present detailed lists of the contents of the Swarm Level 1b Products and brief descriptions of the processing algorithms used in the generation of these data.

  10. ESA Swarm Mission - Level 1b Products

    NASA Astrophysics Data System (ADS)

    Tøffner-Clausen, L.; Floberghagen, R.; Mecozzi, R.; Menard, Y.; Swarm Level 1b Algorithms Team

    2011-12-01

    The ESA Earth Explorer mission, Swarm, is scheduled for launch in July 2012. The objective of the Swarm mission is to provide the best ever survey of the geomagnetic field and its temporal evolution by means of three formation flying spacecrafts in near Earth, polar orbits between 300 and 530 km altitude. The Level 1b Products of the Swarm mission contain time-series of the quality screened, calibrated, corrected, and fully geo-localized measurements of the magnetic field intensity, the magnetic field vector (provided in both instrument and Earth-fixed frames), the plasma density, temperature, and velocity. Additionally, quality screened and pre-calibrated measurements of the non-gravitational accelerations are provided. Geo-localization is performed by 24-channel GPS receivers and by means of unique, three head Advanced Stellar Compasses for high-precision satellite attitude information. The Swarm Level 1b data will be provided in daily products separately for each of the three Swarm spacecrafts. This poster will present detailed lists of the contents of the Swarm Level 1b Products and brief descriptions of the processing algorithms used in the generation of these data.

  11. Interaction of human organic anion transporter polypeptides 1B1 and 1B3 with antineoplastic compounds.

    PubMed

    Marada, Venkata V V R; Flörl, Saskia; Kühne, Annett; Burckhardt, Gerhard; Hagos, Yohannes

    2015-03-06

    Antineoplastic compounds are used in the treatment of a variety of cancers. The effectiveness of an antineoplastic compound to exert its activity is largely dependent on transport proteins involved in the entry of the compound into the cells, and those which drive it out of the cell. Organic anion transporting polypeptide 1B1 (OATP1B1) and organic anion transporting polypeptide 1B3 (OATP1B3), belonging to the SLCO family of proteins, are specifically expressed in the sinusoidal membranes of the liver, and are known to interact with a variety of drugs. The present study deals with the interaction of these proteins with antineoplastic compounds routinely used in cancer chemotherapy. The proteins OATP1B1 and OATP1B3 were functionally characterized in stably transfected human embryonic kidney cells using [(3)H] labeled estrone 3-sulfate and [(3)H] labeled cholecystokinin octapeptide (CCK-8) as substrates, respectively. Substrate uptake experiments performed in the presence of antineoplastic compounds showed that vinblastine and paclitaxel strongly interacted with the OATP1B1 with Ki values of 10.2 μM and 0.84 μM, respectively. OATP1B3 showed highly significant interactions with a variety of antineoplastic compounds including chlorambucil, mitoxantrone, vinblastine, vincristine, paclitaxel and etoposide, with Ki values of 40.6 μM, 3.2 μM, 15.9 μM, 30.6 μM, 1.8 μM and 13.5 μM, respectively. We report several novel interactions of the transporter proteins OATP1B1 and OATP1B3 highlighting the need to investigate their role in drug-drug interactions and cancer chemotherapy.

  12. High-Throughput Screen for Inhibitors of Transglycosylase and/or Transpeptidase Activities of Escherichia coli Penicillin Binding Protein 1b

    PubMed Central

    Chandrakala, B.; Shandil, Radha K.; Mehra, Upasana; Ravishankar, Sudha; Kaur, Parvinder; Usha, Veeraraghavan; Joe, Bina; deSousa, Sunita M.

    2004-01-01

    Penicillin binding protein (PBP) 1b of Escherichia coli has both transglycosylase and transpeptidase activities, which are attractive targets for the discovery of new antibacterial agents. A high-throughput assay that detects inhibitors of the PBPs was described previously, but it cannot distinguish them from inhibitors of the MraY, MurG, and lipid pyrophosphorylase. We report on a method that distinguishes inhibitors of both activities of the PBPs from those of the other three enzymes. Radioactive peptidoglycan was synthesized by using E. coli membranes. Following termination of the reaction the products were analyzed in three ways. Wheat germ agglutinin (WGA)-coated scintillation proximity assay (SPA) beads were added to one set, and the same beads together with a detergent were added to a second set. Type A polyethylenimine-coated WGA-coated SPA beads were added to a third set. By comparison of the results of assays run in parallel under the first two conditions, inhibitors of the transpeptidase and transglycosylase could be distinguished from inhibitors of the other enzymes, as the inhibitors of the other enzymes showed similar inhibitory concentrations (IC50s) under both conditions but the inhibitors of the PBPs showed insignificant inhibition in the absence of detergent. Furthermore, comparison of the results of assays run under conditions two and three enabled the distinction of transpeptidase inhibitors. Penicillin and other β-lactams showed insignificant inhibition with type A beads compared with that shown with WGA-coated SPA beads plus detergent. However, inhibitors of the other four enzymes (tunicamycin, nisin, bacitracin, and moenomycin) showed similar IC50s under both conditions. We show that the main PBP being measured under these conditions is PBP 1b. This screen can be used to find novel transglycosylase or transpeptidase inhibitors. PMID:14693515

  13. High Performance Work Systems and Firm Performance.

    ERIC Educational Resources Information Center

    Kling, Jeffrey

    1995-01-01

    A review of 17 studies of high-performance work systems concludes that benefits of employee involvement, skill training, and other high-performance work practices tend to be greater when new methods are adopted as part of a consistent whole. (Author)

  14. Interferon Beta-1b Injection

    MedlinePlus

    Interferon beta-1b injection is used to reduce episodes of symptoms in patients with relapsing-remitting (course ... and problems with vision, speech, and bladder control). Interferon beta-1b is in a class of medications ...

  15. Interferon Gamma-1b Injection

    MedlinePlus

    Interferon gamma-1b injection is used to reduce the frequency and severity of serious infections in people ... with severe, malignant osteopetrosis (an inherited bone disease). Interferon gamma-1b is in a class of medications ...

  16. Combined treatment of betulinic acid, a PTP1B inhibitor, with Orthosiphon stamineus extract decreases body weight in high-fat-fed mice.

    PubMed

    Choi, Yoon-Jung; Park, So-Young; Kim, Jong-Yeon; Won, Kyu-Chang; Kim, Bo-Ra; Son, Jong-Keun; Lee, Seung-Ho; Kim, Yong-Woon

    2013-01-01

    Leptin resistance is a common feature of obesity and is accompanied by hyperleptinemia. Although leptin sensitizers improve leptin resistance, they also decrease plasma leptin levels that attenuate the leptin-associated antiobesity effect. We hypothesized that the combinational treatment of leptin sensitizer and endogenous leptin expression stimulant would synergistically induce an antiobesity effect in high-fat-fed obese animals. Betulinic acid (BA) isolated from Saussurea lappa suppressed the hypothalamic protein tyrosine phosphatase 1B in mice and enhanced the antiobesity effect of leptin in obese rats. Ethanol extract of Orthosiphon stamineus (OS) induced leptin expressions in both 3T3-L1 adipocytes and mice in a dose-dependent manner. To evaluate our hypothesis, we treated obese mice induced by 6 weeks of high-fat-diet feeding with BA and OS for 2 weeks. Although BA or OS alone did not decrease body weight in obese mice, the combinational treatment of BA and OS decreased body weight significantly compared to either BA- or OS-treated obese mice. These results suggest that combinational treatment of BA and OS would be effective for the treatment of obesity.

  17. High prevalence of HBV infection, detection of subgenotypes F1b, A2, and D4, and differential risk factors among Mexican risk populations with low socioeconomic status.

    PubMed

    Jose-Abrego, Alexis; Panduro, Arturo; Fierro, Nora A; Roman, Sonia

    2017-08-09

    Hepatitis B virus (HBV) infection may be underestimated among high-risk individuals in regions of low HBs antigenemia. This study aimed to assess HBV serological markers, genotypes, and risk factors in Mexican patients with risk of HBV infection and low socioeconomic status. Demographics, clinical, and risk factor data were collected in patients with HIV (n = 289), HCV (n = 243), deferred blood donors (D-BD) (n = 83), and two native populations, Mixtecos (n = 57) and Purepechas (n = 44). HBV infection was assessed by HBsAg, anti-HBc, and HBV-DNA testing. Overall, patients had low education and very-low income. Totally, HBsAg prevalence was 16.5% (113/684) ranging from 0.7% (HCV) to 37.3% (D-BD), while anti-HBc was 30.2% (207/684). Among 52 sequences, genotypes H (n = 34, 65.4%), G (n = 4, 7.7%), subgenotypes F1b (n = 7, 13.5%), A2 (n = 6, 11.5%), and D4 (n = 1, 1.9%) were detected. Surgeries, sexual promiscuity, and blood transfusions had a differential pattern of distribution. In HCV patients, single (OR = 5.84, 95%Cl 1.91-17.80, P = 0.002), MSM (OR = 4.80, 95%Cl 0.75-30.56, P = 0.097), and IDU (OR = 2.93, 95%CI 1.058-8.09, P = 0.039) were predictors for HBV infection. While IDU (OR = 2.68, 95%CI 1.08-6.61, P = 0.033) and MSM (OR = 2.64, 95%CI 1.39-5.04, P = 0.003) were predictors in HIV patients. In this group, MSM was associated with HBsAg positivity (OR = 3.45, 95%CI 1.48-8.07, P = 0.004) and IDU with anti-HBc positivity (OR = 5.12, 95%CI 2.05-12.77, P < 0.001). In conclusion, testing with a combined approach of three different HBV markers, a high prevalence of HBV infection, a differential distribution of HBV genotypes, including subgenotypes F1b, A2, and D4, as well as risk factors in low-income Mexican risk groups were detected. © 2017 Wiley Periodicals, Inc.

  18. Commoditization of High Performance Storage

    SciTech Connect

    Studham, Scott S.

    2004-04-01

    The commoditization of high performance computers started in the late 80s with the attack of the killer micros. Previously, high performance computers were exotic vector systems that could only be afforded by an illustrious few. Now everyone has a supercomputer composed of clusters of commodity processors. A similar commoditization of high performance storage has begun. Commodity disks are being used for high performance storage, enabling a paradigm change in storage and significantly changing the price point of high volume storage.

  19. gr/gr-DAZ2-DAZ4-CDY1b deletion is a high-risk factor for male infertility in Tunisian population.

    PubMed

    Ghorbel, Myriam; Baklouti-Gargouri, Siwar; Keskes, Rim; Chakroun, Nozha; Sellami, Afifa; Fakhfakh, Faiza; Ammar-Keskes, Leila

    2016-10-30

    The azoospermia factor c (AZFc) region harbors multi-copy genes that are expressed in the testis. Deletions of this region lead to reduced copy numbers of these genes. In this present study we aimed to determine the frequency of AZFc subdeletion in infertile and fertile men from Tunisia and to identify whether deletions of DAZ and CDY1 gene copies are deleterious on spermatogenesis and on semen quality. We studied a group of 241 infertile men and 115 fertile healthy males using a sequence tagged site (STS)±method. To gain insight into the molecular basis of the heterogeneous phenotype observed in men with the deletion we defined the type of DAZ and CDY1 genes deleted. We reported in the present study and for the first time a new type of AZFc deletion (gr/gr-DAZ2-DAZ4-CDY1b) and hypothesis that this new deletion is the result of two successive events. We also demonstrated that this deletion constitutes a relative high-risk factor for male infertility in Tunisian population.

  20. Treatment with sofosbuvir and ledipasvir without ribavirin for 12 weeks is highly effective for recurrent hepatitis C virus genotype 1b infection after living donor liver transplantation: a Japanese multicenter experience.

    PubMed

    Ueda, Yoshihide; Ikegami, Toru; Akamatsu, Nobuhisa; Soyama, Akihiko; Shinoda, Masahiro; Goto, Ryoichi; Okajima, Hideaki; Yoshizumi, Tomoharu; Taketomi, Akinobu; Kitagawa, Yuko; Eguchi, Susumu; Kokudo, Norihiro; Uemoto, Shinji; Maehara, Yoshihiko

    2017-08-01

    The optimal therapy for recurrent hepatitis C virus (HCV) infection after liver transplantation has not yet been established. This study aimed to clarify the efficacy and safety of interferon-free therapy with sofosbuvir and ledipasvir without ribavirin for 12 weeks in Japanese patients with HCV genotype 1b infection after living donor liver transplantation. A cohort study of living donor liver transplant recipients with recurrent HCV genotype 1b infection treated with sofosbuvir (400 mg/day) and ledipasvir (90 mg/day) was performed at six liver transplant centers in Japan. Fifty-four patients were treated with sofosbuvir and ledipasvir. Thirty-eight patients (70%) were treatment experienced, including 17 patients who had undergone prior direct-acting-antiviral-based triple therapy. Ten patients had resistance-associated substitutions at L31 or Y93 in the NS5A region of the HCV genome. Fifty-three patients completed the 12-week treatment protocol; treatment was discontinued in one patient who developed pneumonia at 4 weeks and died thereafter. All 53 patients who completed the treatment regimen achieved a sustained virological response 12 weeks after completion of treatment. Treatment was well tolerated in most patients, but seven patients developed serious adverse events, including hemorrhagic duodenal ulcers (n = 3), infection (n = 2), pleural effusion (n = 1), and alveolar hemorrhage (n = 1). Sofosbuvir and ledipasvir treatment without ribavirin for 12 weeks was highly effective in achieving a sustained virological response in Japanese patients who developed recurrent HCV genotype 1b infection after living donor liver transplantation.

  1. High performance collectors

    NASA Astrophysics Data System (ADS)

    Ogawa, H.; Hozumi, S.; Mitsumata, T.; Yoshino, K.; Aso, S.; Ebisu, K.

    1983-04-01

    Materials and structures used for flat plate solar collectors and evacuated tubular collectors were examined relative to their overall performance to project effectiveness for building heating and cooling and the feasibility of use for generating industrial process heat. Thermal efficiencies were calculated for black paint single glazed, selective surface single glazed, and selective surface double glazed flat plate collectors. The efficiencies of a single tube and central tube accompanied by two side tube collectors were also studied. Techniques for extending the lifetimes of the collectors were defined. The selective surface collectors proved to have a performance superior to other collectors in terms of the average annual energy delivered. Addition of a black chrome-coated fin system to the evacuated collectors produced significant collection efficiency increases.

  2. High Performance Arcjet Engines

    NASA Technical Reports Server (NTRS)

    Kennel, Elliot B.; Ivanov, Alexey Nikolayevich; Nikolayev, Yuri Vyacheslavovich

    1994-01-01

    This effort sought to exploit advanced single crystal tungsten-tantalum alloy material for fabrication of a high strength, high temperature arcjet anode. The use of this material is expected to result in improved strength, temperature resistance, and lifetime compared to state of the art polycrystalline alloys. In addition, the use of high electrical and thermal conductivity carbon-carbon composites was considered, and is believed to be a feasible approach. Highly conductive carbon-carbon composite anode capability represents enabling technology for rotating-arc designs derived from the Russian Scientific Research Institute of Thermal Processes (NIITP) because of high heat fluxes at the anode surface. However, for US designs the anode heat flux is much smaller, and thus the benefits are not as great as in the case of NIITP-derived designs. Still, it does appear that the tensile properties of carbon-carbon can be even better than those of single crystal tungsten alloys, especially when nearly-single-crystal fibers such as vapor grown carbon fiber (VGCF) are used. Composites fabricated from such materials must be coated with a refractory carbide coating in order to ensure compatibility with high temperature hydrogen. Fabrication of tungsten alloy single crystals in the sizes required for fabrication of an arcjet anode has been shown to be feasible. Test data indicate that the material can be expected to be at least the equal of W-Re-HfC polycrystalline alloy in terms of its tensile properties, and possibly superior. We are also informed by our colleagues at Scientific Production Association Luch (NP0 Luch) that it is possible to use Russian technology to fabricate polycrystalline W-Re-HfC or other high strength alloys if desired. This is important because existing engines must rely on previously accumulated stocks of these materials, and a fabrication capability for future requirements is not assured.

  3. High Performance Organic Semiconductors

    DTIC Science & Technology

    2012-07-31

    the polymers in o-DCB. Grazing incidence X-ray diffraction (GIXD) studies performed on oADT-dTDPP thin films shows distinct out-of- plane (h00...have helped to promote interchain interactions and the formation of lamellar order. In- plane diffraction intensity profile along qxy shows multiple...perpendicular to the substrate, a motif that may give rise to better in- plane charge transport properties than previous less-ordered ADT-containing polymer

  4. High Performance Hollow Projectiles

    DTIC Science & Technology

    Development of hollow projectiles was first advocated to achieve ’silent’ (low pressure signal) projectiles having higher performance. Although the...present effort concentrates on small arms (specifically 7.62 mm), the confirmed fundamental theory applies to all sizes of hollow projectiles. The...report can thus serve as a basis for (1) evaluating specific hollow projectile developments and (2) formulating programs to develop a wide spectrum of

  5. High Performance Magnets

    DTIC Science & Technology

    2000-03-29

    Our efforts in this project were focused on three different materials, namely; interstitial Sm-Fe carbides and nitrides, high energy product Nd2Fe14B ...magnets with MgO addition, and nanocomposite Nd2Fe14B /alpha-Fe consisting of a fine mixture of hard and soft phases. In the Sm-Fe carbides and

  6. High Performance YBCO Films

    DTIC Science & Technology

    1992-07-01

    growing high quality MgO films on SrF2 substrates is the oxygen partial pressure during the growth. The x-ray data presented in Fig. 13 indicates a...fluo-ide and quartz substrates. The best result with two buffer layers (MgO and YSZ) on SrF2 was an onset temperature (Tc) of 82K and a transition...With a YSZ buffer an onset temperature of 85K and a transition width of 5K was achieved. Recent success was demonstrated by Neocera ( under a NASA

  7. High performance steam development

    SciTech Connect

    Duffy, T.; Schneider, P.

    1995-10-01

    Over 30 years ago U.S. industry introduced the world`s highest temperature (1200{degrees}F at 5000 psig) and most efficient power plant, the Eddystone coal-burning steam plant. The highest alloy material used in the plant was 316 stainless steel. Problems during the first few years of operation caused a reduction in operating temperature to 1100{degrees}F which has generally become the highest temperature used in plants around the world. Leadership in high temperature steam has moved to Japan and Europe over the last 30 years.

  8. High Voltage SPT Performance

    NASA Technical Reports Server (NTRS)

    Manzella, David; Jacobson, David; Jankovsky, Robert

    2001-01-01

    A 2.3 kW stationary plasma thruster designed to operate at high voltage was tested at discharge voltages between 300 and 1250 V. Discharge specific impulses between 1600 and 3700 sec were demonstrated with thrust between 40 and 145 mN. Test data indicated that discharge voltage can be optimized for maximum discharge efficiency. The optimum discharge voltage was between 500 and 700 V for the various anode mass flow rates considered. The effect of operating voltage on optimal magnet field strength was investigated. The effect of cathode flow rate on thruster efficiency was considered for an 800 V discharge.

  9. Tough high performance composite matrix

    NASA Technical Reports Server (NTRS)

    Pater, Ruth H. (Inventor); Johnston, Norman J. (Inventor)

    1994-01-01

    This invention is a semi-interpentrating polymer network which includes a high performance thermosetting polyimide having a nadic end group acting as a crosslinking site and a high performance linear thermoplastic polyimide. Provided is an improved high temperature matrix resin which is capable of performing in the 200 to 300 C range. This resin has significantly improved toughness and microcracking resistance, excellent processability, mechanical performance, and moisture and solvent resistances.

  10. High performance alloy electroforming

    NASA Technical Reports Server (NTRS)

    Malone, G. A.; Winkelman, D. M.

    1989-01-01

    Electroformed copper and nickel are used in structural applications for advanced propellant combustion chambers. An improved process has been developed by Bell Aerospace Textron, Inc. wherein electroformed nickel-manganese alloy has demonstrated superior mechanical and thermal stability when compared to previously reported deposits from known nickel plating processes. Solution chemistry and parametric operating procedures are now established and material property data is established for deposition of thick, large complex shapes such as the Space Shuttle Main Engine. The critical operating variables are those governing the ratio of codeposited nickel and manganese. The deposition uniformity which in turn affects the manganese concentration distribution is affected by solution resistance and geometric effects as well as solution agitation. The manganese concentration in the deposit must be between 2000 and 3000 ppm for optimum physical properties to be realized. The study also includes data regarding deposition procedures for achieving excellent bond strength at an interface with copper, nickel-manganese or INCONEL 718. Applications for this electroformed material include fabrication of complex or re-entry shapes which would be difficult or impossible to form from high strength alloys such as INCONEL 718.

  11. ADH1B Arg47His polymorphism is associated with esophageal cancer risk in high-incidence Asian population: evidence from a meta-analysis.

    PubMed

    Zhang, Guohong; Mai, Ruiqin; Huang, Bo

    2010-10-27

    Incidence of Esophageal squamous cell carcinoma (ESCC) is prevalent in Asian populations, especially in the ones from the "Asian esophageal cancer belt" along the Silk Road and the ones from East Asia (including Japan). Silk Road and Eastern Asia population genetics are relevant to the ancient population migration from central China. The Arg47His (rs1229984) polymorphism of ADH1B is the highest in East Asians, and ancient migrations along the Silk Road were thought to be contributive to a frequent ADH1B*47His allele in Central Asians. This polymorphism was identified as responsible for susceptibility in the first large-scale genome-wide association study of ESCC and that's explained by its modulation of alcohol oxidization capability. To investigate the association of ADH1B Arg47His with ESCC in Asian populations under a common ancestry scenario of the susceptibility loci, we combined all available studies into a meta-analysis. A dataset composed of 4,220 cases and 8,946 controls from twelve studies of Asian populations was analyzed for ADH1B Arg47His association with ESCC and its interactions with alcohol drinking and ALDH2 Glu504Lys. Heterogeneity among studies and their publication bias were also tested. The ADH1B*47Arg allele was found to be associated to increased risk of ESCC, with the odds ratios (OR) being 1.62 (95% CI: 1.49-1.76) and 3.86 (2.96-5.03) for the His/Arg and the Arg/Arg genotypes, respectively. When compared with the His/His genotype of non-drinkers, the Arg/Arg genotype can interact with alcohol drinking and greatly increase the risk of ESCC (OR = 20.69, 95%CI: 5.09-84.13). Statistical tests also showed gene-gene interaction of ADH1B Arg+ with ALDH2 Lys+ can bring more risk to ESCC (OR  = 13.46, 95% CI: 2.32-78.07). Revealed by this meta-analysis, ADH1B*47Arg as a common ancestral allele can significantly increase the risk of ESCC in Asians, especially when coupled with alcohol drinking or the ALDH2*504Lys allele.

  12. Environmental Performance Report 2011: Annual Site Environmental Report per the U.S. Department of Energy Order 231.1B (Management Report)

    SciTech Connect

    Not Available

    2012-09-01

    Purpose: NREL's Environmental Performance Report provides a description of the lab's environmental activities for 2011 including information on environmental and sustainability performance, environmental compliance activities and status, and environmental protection programs, highlights, and successes.

  13. The SLCO1B1 rs4149032 Polymorphism Is Highly Prevalent in South Africans and Is Associated with Reduced Rifampin Concentrations: Dosing Implications▿

    PubMed Central

    Chigutsa, Emmanuel; Visser, Marianne E.; Swart, Elizabeth C.; Denti, Paolo; Pushpakom, Sudeep; Egan, Deirdre; Holford, Nicholas H. G.; Smith, Peter J.; Maartens, Gary; Owen, Andrew; McIlleron, Helen

    2011-01-01

    Among patients with tuberculosis, rifampin plasma concentrations and sputum conversion rates have been reported to be lower in Africans. Rifampin is a substrate of P-glycoprotein (coded for by the ABCB1 gene) and organic anion-transporting polypeptide 1B1 (coded for by SLCO1B1). The objectives were to identify genetic polymorphisms of drug transporters and the transcriptional regulators pregnane X receptor (PXR) and constitutive androstane receptor (CAR) with an impact on rifampin pharmacokinetics in South Africans. Fifty-seven patients with tuberculosis from Cape Town underwent pharmacokinetic sampling during treatment with rifampin, pyrazinamide, isoniazid, and ethambutol. DNA was genotyped for ABCB1, SLCO1B1, PXR, and CAR polymorphisms by using real-time PCR. NONMEM was used for data analysis. The allele frequency of the SLCO1B1 rs4149032 polymorphism was 0.70. Patients heterozygous and homozygous for this polymorphism had reductions in the bioavailability (and, thus, the area under the curve [AUC]) of rifampin of 18% and 28%, respectively. Simulations showed that increasing the daily rifampin dose by 150 mg in patients with the polymorphism would result in plasma concentrations similar to those of wild-type individuals and reduce the percentage of patients with peak plasma concentrations (Cmax) below 8 mg/liter from 63% to 31%. ABCB1, PXR, and CAR polymorphisms were not associated with differences in rifampin pharmacokinetics. SLCO1B1 rs4149032 was present in most patients and was associated with substantially reduced rifampin exposure. These data suggest that the standard recommended dose of rifampin should be reconsidered for South Africans. PMID:21709081

  14. HvHMA2, a P(1B)-ATPase from barley, is highly conserved among cereals and functions in Zn and Cd transport.

    PubMed

    Mills, Rebecca F; Peaston, Kerry A; Runions, John; Williams, Lorraine E

    2012-01-01

    Manipulation of crops to improve their nutritional value (biofortification) and optimisation of plants for removal of toxic metals from contaminated soils (phytoremediation) are major goals. Identification of membrane transporters with roles in zinc and cadmium transport would be useful for both aspects. The P(1B)-ATPases play important roles in heavy metal allocation and detoxification in Arabidopsis and it is now important to elucidate their roles in monocots. We identified nine P(1B)-ATPases in barley and this study focuses on the functional characterization of HvHMA2, providing evidence for its role in heavy metal transport. HvHMA2 was cloned using information from EST analysis and 5' RACE. It possesses the conserved aspartate that is phosphorylated during the reaction cycle of P-type pumps and has motifs and key residues characteristic of P(1B)-ATPases, falling into the P(1B-2) subclass. Homologous sequences occur in three major sub-families of the Poaceae (Gramineae). Heterologous expression in Saccharomyces cerevisiae demonstrates that HvHMA2 functions as a Zn and Cd pump. Mutagenesis studies show that proposed cation coordination sites of the P(1B-2) pumps are crucial for the metal responses conferred by HvHMA2 in yeast. HvHMA2 expression suppresses the Zn-deficient phenotype of the Arabidopsis hma2hma4 mutant indicating that HvHMA2 functions as a Zn pump in planta and could play a role in root to shoot Zn transport. When expressed in Arabidopsis, HvHMA2 localises predominantly to the plasma membrane.

  15. Physiological characterization of Mycobacterium sp. strain 1B isolated from a bacterial culture able to degrade high-molecular-weight polycyclic aromatic hydrocarbons.

    PubMed

    Dandie, C E; Thomas, S M; Bentham, R H; McClure, N C

    2004-01-01

    The aim of this study was to further characterize a bacterial culture (VUN 10,010) capable of benzo[a]pyrene cometabolism. The bacterial culture, previously characterized as a pure culture of Stenotrophomonas maltophilia (VUN 10,010), was found to also contain another bacterial species (Mycobacterium sp. strain 1B), capable of degrading a similar range of PAH substrates. Analysis of its 16S rRNA gene sequence and growth characteristics revealed the strain to be a fast-growing Mycobacterium sp., closely related to other previously isolated PAH and xenobiotic-degrading mycobacterial strains. Comparison of the PAH-degrading characteristics of Mycobacterium sp. strain 1B with those of S. maltophilia indicated some similarities (ability to degrade phenanthrene and pyrene), but some differences were also noted (S. maltophilia able to degrade fluorene, but not fluoranthene, whereas Mycobacterium sp. strain 1B can degrade fluoranthene, but not fluorene). Unlike the S. maltophilia culture, there was no evidence of benzo[a]pyrene degradation by Mycobacterium sp. strain 1B, even in the presence of other PAHs (ie pyrene) as co-metabolic substrates. Growth of Mycobacterium sp. strain 1B on other organic carbon sources was also limited compared with the S. maltophilia culture. This study isolated a Mycobacterium strain from a bacterial culture capable of benzo[a]pyrene cometabolism. The Mycobacterium strain displays different PAH-degrading characteristics to those described previously for the PAH-degrading bacterial culture. It is unclear what role the two bacterial strains play in benzo[a]pyrene cometabolism, as the Mycobacterium strain does not appear to have endogenous benzo[a]pyrene degrading ability. This study describes the isolation and characterization of a novel PAH-degrading Mycobacterium strain from a PAH-degrading culture. Further studies utilizing this strain alone, and in combination with other members of the consortium, will provide insight into the diverse roles

  16. High Performance Computing at NASA

    NASA Technical Reports Server (NTRS)

    Bailey, David H.; Cooper, D. M. (Technical Monitor)

    1994-01-01

    The speaker will give an overview of high performance computing in the U.S. in general and within NASA in particular, including a description of the recently signed NASA-IBM cooperative agreement. The latest performance figures of various parallel systems on the NAS Parallel Benchmarks will be presented. The speaker was one of the authors of the NAS (National Aerospace Standards) Parallel Benchmarks, which are now widely cited in the industry as a measure of sustained performance on realistic high-end scientific applications. It will be shown that significant progress has been made by the highly parallel supercomputer industry during the past year or so, with several new systems, based on high-performance RISC processors, that now deliver superior performance per dollar compared to conventional supercomputers. Various pitfalls in reporting performance will be discussed. The speaker will then conclude by assessing the general state of the high performance computing field.

  17. Effects of the novel high-affinity 5-HT(1B/1D)-receptor ligand frovatriptan in human isolated basilar and coronary arteries.

    PubMed

    Parsons, A A; Raval, P; Smith, S; Tilford, N; King, F D; Kaumann, A J; Hunter, J

    1998-08-01

    The contractile actions of the novel high-affinity 5-hydroxytryptamine (5-HT(1B/1D)) ligand, frovatriptan (formerly VML 251/SB-209509) were investigated in human isolated basilar and coronary arteries in which the endothelium had been removed. Basilar arteries were obtained post mortem, and coronary arteries were obtained from patients undergoing heart transplant (recipient) or from donor hearts that were not suitable for transplant. Frovatriptan was a potent contractile agent in isolated basilar artery with a -log mean effective concentration (EC50) value of 7.86 +/- 0.07 and intrinsic activity of 1.25 +/- 0.10 relative to 5-HT (n = 4). Frovatriptan was 8.5-fold more potent than sumatriptan, which produced a -log EC50 value of 6.93 +/- 0.09 and intrinsic activity 11.1 +/- 0.08 relative to 5-HT (n = 4). In coronary arteries, frovatriptan produced contraction with -log EC50 values of 7.38 +/- 0.12 and 7.81 +/- 0.2 in recipient (n = 7) and donor (n = 3) arteries, respectively. The relative degree of contraction of frovatriptan was lower than that of 5-HT, with relative intrinsic activities of 0.42 +/- 0.06 and 0.40 +/- 0.09, respectively. Sumatriptan produced contraction of human recipient and donor arteries with -log EC50 values (intrinsic activity) of 6.57 +/- 0.13 (0.79 +/- 0.27; n = 6) and 7.35 (1.41; n = 2), respectively. Furthermore, marked bell-shaped responses were apparent for frovatriptan in coronary arteries, with relaxation occurring at concentrations >6 microM in some tissues. In contrast, no bell-shaped concentration-response curves were apparent for sumatriptan or 5-HT. Threshold concentrations for frovatriptan-induced contractions were also different between basilar (>2 nM) and coronary arteries (>20 nM). No separation of threshold activity was observed with sumatriptan or 5-HT. These data show that frovatriptan produces constriction of human isolated basilar and coronary arteries. However, frovatriptan produces a complex pharmacologic response in the

  18. High Performance Fortran: An overview

    SciTech Connect

    Zosel, M.E.

    1992-12-23

    The purpose of this paper is to give an overview of the work of the High Performance Fortran Forum (HPFF). This group of industry, academic, and user representatives has been meeting to define a set of extensions for Fortran dedicated to the special problems posed by a very high performance computers, especially the new generation of parallel computers. The paper describes the HPFF effort and its goals and gives a brief description of the functionality of High Performance Fortran (HPF).

  19. GIFTS SM EDU Level 1B Algorithms

    NASA Technical Reports Server (NTRS)

    Tian, Jialin; Gazarik, Michael J.; Reisse, Robert A.; Johnson, David G.

    2007-01-01

    The Geosynchronous Imaging Fourier Transform Spectrometer (GIFTS) SensorModule (SM) Engineering Demonstration Unit (EDU) is a high resolution spectral imager designed to measure infrared (IR) radiances using a Fourier transform spectrometer (FTS). The GIFTS instrument employs three focal plane arrays (FPAs), which gather measurements across the long-wave IR (LWIR), short/mid-wave IR (SMWIR), and visible spectral bands. The raw interferogram measurements are radiometrically and spectrally calibrated to produce radiance spectra, which are further processed to obtain atmospheric profiles via retrieval algorithms. This paper describes the GIFTS SM EDU Level 1B algorithms involved in the calibration. The GIFTS Level 1B calibration procedures can be subdivided into four blocks. In the first block, the measured raw interferograms are first corrected for the detector nonlinearity distortion, followed by the complex filtering and decimation procedure. In the second block, a phase correction algorithm is applied to the filtered and decimated complex interferograms. The resulting imaginary part of the spectrum contains only the noise component of the uncorrected spectrum. Additional random noise reduction can be accomplished by applying a spectral smoothing routine to the phase-corrected spectrum. The phase correction and spectral smoothing operations are performed on a set of interferogram scans for both ambient and hot blackbody references. To continue with the calibration, we compute the spectral responsivity based on the previous results, from which, the calibrated ambient blackbody (ABB), hot blackbody (HBB), and scene spectra can be obtained. We now can estimate the noise equivalent spectral radiance (NESR) from the calibrated ABB and HBB spectra. The correction schemes that compensate for the fore-optics offsets and off-axis effects are also implemented. In the third block, we developed an efficient method of generating pixel performance assessments. In addition, a

  20. High Performance Thin Layer Chromatography.

    ERIC Educational Resources Information Center

    Costanzo, Samuel J.

    1984-01-01

    Clarifies where in the scheme of modern chromatography high performance thin layer chromatography (TLC) fits and why in some situations it is a viable alternative to gas and high performance liquid chromatography. New TLC plates, sample applications, plate development, and instrumental techniques are considered. (JN)

  1. High Performance Thin Layer Chromatography.

    ERIC Educational Resources Information Center

    Costanzo, Samuel J.

    1984-01-01

    Clarifies where in the scheme of modern chromatography high performance thin layer chromatography (TLC) fits and why in some situations it is a viable alternative to gas and high performance liquid chromatography. New TLC plates, sample applications, plate development, and instrumental techniques are considered. (JN)

  2. Strv1-b Flight Experiment

    NASA Technical Reports Server (NTRS)

    1992-01-01

    The Space Technology Research Vehicle (STRV-1b) is discussed. The STRV-1b is a small (100 watt) satellite flown by the British Defense Research Agency (DRA). The mission goal is to fly new and emerging space technologies at a more reasonable cost in a short-term timeframe. The STRV-1b's orbit can be described as a geosynchronous transfer orbit (GTO) with a perigee of 200 km, an apogee of 36,000 km (geosynchronous earth orbit (GEO)), and a period of 10.5 hours. The Phillips Laboratory plans to use the STRV-1b to test up to 20 advanced experimental photovoltaic cells. The discussion is presented in vugraph form.

  3. High Performance Flexible Thermal Link

    NASA Astrophysics Data System (ADS)

    Sauer, Arne; Preller, Fabian

    2014-06-01

    The paper deals with the design and performance verification of a high performance and flexible carbon fibre thermal link.Project goal was to design a space qualified thermal link combining low mass, flexibility and high thermal conductivity with new approaches regarding selected materials and processes. The idea was to combine the advantages of existing metallic links regarding flexibility and the thermal performance of high conductive carbon pitch fibres. Special focus is laid on the thermal performance improvement of matrix systems by means of nano-scaled carbon materials in order to improve the thermal performance also perpendicular to the direction of the unidirectional fibres.One of the main challenges was to establish a manufacturing process which allows handling the stiff and brittle fibres, applying the matrix and performing the implementation into an interface component using unconventional process steps like thermal bonding of fibres after metallisation.This research was funded by the German Federal Ministry for Economic Affairs and Energy (BMWi).

  4. High Performance Networks for High Impact Science

    SciTech Connect

    Scott, Mary A.; Bair, Raymond A.

    2003-02-13

    This workshop was the first major activity in developing a strategic plan for high-performance networking in the Office of Science. Held August 13 through 15, 2002, it brought together a selection of end users, especially representing the emerging, high-visibility initiatives, and network visionaries to identify opportunities and begin defining the path forward.

  5. Aldo-keto Reductase 1B15 (AKR1B15)

    PubMed Central

    Weber, Susanne; Salabei, Joshua K.; Möller, Gabriele; Kremmer, Elisabeth; Bhatnagar, Aruni; Adamski, Jerzy; Barski, Oleg A.

    2015-01-01

    Aldo-keto reductases (AKRs) comprise a superfamily of proteins involved in the reduction and oxidation of biogenic and xenobiotic carbonyls. In humans, at least 15 AKR superfamily members have been identified so far. One of these is a newly identified gene locus, AKR1B15, which clusters on chromosome 7 with the other human AKR1B subfamily members (i.e. AKR1B1 and AKR1B10). We show that alternative splicing of the AKR1B15 gene transcript gives rise to two protein isoforms with different N termini: AKR1B15.1 is a 316-amino acid protein with 91% amino acid identity to AKR1B10; AKR1B15.2 has a prolonged N terminus and consists of 344 amino acid residues. The two gene products differ in their expression level, subcellular localization, and activity. In contrast with other AKR enzymes, which are mostly cytosolic, AKR1B15.1 co-localizes with the mitochondria. Kinetic studies show that AKR1B15.1 is predominantly a reductive enzyme that catalyzes the reduction of androgens and estrogens with high positional selectivity (17β-hydroxysteroid dehydrogenase activity) as well as 3-keto-acyl-CoA conjugates and exhibits strong cofactor selectivity toward NADP(H). In accordance with its substrate spectrum, the enzyme is expressed at the highest levels in steroid-sensitive tissues, namely placenta, testis, and adipose tissue. Placental and adipose expression could be reproduced in the BeWo and SGBS cell lines, respectively. In contrast, AKR1B15.2 localizes to the cytosol and displays no enzymatic activity with the substrates tested. Collectively, these results demonstrate the existence of a novel catalytically active AKR, which is associated with mitochondria and expressed mainly in steroid-sensitive tissues. PMID:25577493

  6. High-Performance Liquid Chromatography

    NASA Astrophysics Data System (ADS)

    Reuhs, Bradley L.; Rounds, Mary Ann

    High-performance liquid chromatography (HPLC) developed during the 1960s as a direct offshoot of classic column liquid chromatography through improvements in the technology of columns and instrumental components (pumps, injection valves, and detectors). Originally, HPLC was the acronym for high-pressure liquid chromatography, reflecting the high operating pressures generated by early columns. By the late 1970s, however, high-performance liquid chromatography had become the preferred term, emphasizing the effective separations achieved. In fact, newer columns and packing materials offer high performance at moderate pressure (although still high pressure relative to gravity-flow liquid chromatography). HPLC can be applied to the analysis of any compound with solubility in a liquid that can be used as the mobile phase. Although most frequently employed as an analytical technique, HPLC also may be used in the preparative mode.

  7. DRM/GREMLIN (CKTSF1B1) maps to human chromosome 15 and is highly expressed in adult and fetal brain.

    PubMed

    Topol, L Z; Modi, W S; Koochekpour, S; Blair, D G

    2000-01-01

    We have mapped and characterized the human homolog of Drm/Gremlin (CKTFS1B1), a member of a family of BMP antagonists that have been linked to both developmental and transformation-related functions. By screening a human cDNA library, we isolated a 3.3-kb cDNA containing the 552-bp region encoding the human DRM protein. CKTFS1B1 was localized on human chromosome 15q13--> q15 by somatic cell hybrid analysis and, more precisely, using radiation hybrids, to a region of markers linked to SGNE1, secretory granule neuroendocrine protein 1 and RYR3, the ryanodyne receptor 3. Northern blot analysis showed the presence of a single DRM-specific mRNA expressed in different human tissues, including brain, ovary, intestine and colon. In the brain, DRM expression is associated with the region localized around the internal capsule in the large subcortical nuclei. DRM appears to be predominantly expressed in normal cells and tissues, including normal neurons, astrocytes and fibroblasts. Interestingly, we detected DRM expression in normal cells obtained from several patients, but not in tumor cell lines established from the same patients. The data suggest that down-regulation of DRM is associated with tumor progression, and support the hypothesis that human DRM may play an important role during both neuroembryological development and carcinogenesis. Copyright 2000 S. Karger AG, Basel.

  8. Multilayer high performance insulation materials

    NASA Technical Reports Server (NTRS)

    Stuckey, J. M.

    1971-01-01

    A number of tests are required to evaluate both multilayer high performance insulation samples and the materials that comprise them. Some of the techniques and tests being employed for these evaluations and some of the results obtained from thermal conductivity tests, outgassing studies, effect of pressure on layer density tests, hypervelocity impact tests, and a multilayer high performance insulation ambient storage program at the Kennedy Space Center are presented.

  9. KELT-1b: A STRONGLY IRRADIATED, HIGHLY INFLATED, SHORT PERIOD, 27 JUPITER-MASS COMPANION TRANSITING A MID-F STAR

    SciTech Connect

    Siverd, Robert J.; Pepper, Joshua; Stassun, Keivan G.; Beatty, Thomas G.; Scott Gaudi, B.; Eastman, Jason D.; Street, Rachel; Fulton, Benjamin J.; Collins, Karen; Bieryla, Allyson; Latham, David W.; Berlind, Perry; Calkins, Michael L.; Esquerdo, Gilbert A.; Furesz, Gabor; Geary, John C.; Buchhave, Lars A.; Jensen, Eric L. N.; Crepp, Justin R.; DePoy, D. L.; and others

    2012-12-20

    We present the discovery of KELT-1b, the first transiting low-mass companion from the wide-field Kilodegree Extremely Little Telescope-North (KELT-North) transit survey. A joint analysis of the spectroscopic, radial velocity, and photometric data indicates that the V = 10.7 primary is a mildly evolved mid-F star with T{sub eff} = 6516 {+-} 49 K, log g 4.228{sup +0.014}{sub -0.021}, and [Fe/H] = 0.052 {+-} 0.079, with an inferred mass M{sub *} = 1.335 {+-} 0.063 M{sub Sun} and radius R{sub *} 1.471{sup +0.045}{sub -0.035} R{sub Sun }. The companion is a low-mass brown dwarf or a super-massive planet with mass M{sub P} = 27.38 {+-} 0.93 M{sub Jup} and radius R{sub P} = 1.116{sup +0.038}{sub -0.029} R{sub Jup}. The companion is on a very short ({approx}29 hr) period circular orbit, with an ephemeris T{sub c} (BJD{sub TDB}) = 2455909.29280 {+-} 0.00023 and P = 1.217501 {+-} 0.000018 days. KELT-1b receives a large amount of stellar insolation, resulting in an estimated equilibrium temperature assuming zero albedo and perfect redistribution of T{sub eq} = 2423{sup +34}{sub -27} K. Comparison with standard evolutionary models suggests that the radius of KELT-1b is likely to be significantly inflated. Adaptive optics imaging reveals a candidate stellar companion to KELT-1 with a separation of 588 {+-} 1 mas, which is consistent with an M dwarf if it is at the same distance as the primary. Rossiter-McLaughlin measurements during transit imply a projected spin-orbit alignment angle {lambda} = 2 {+-} 16 deg, consistent with a zero obliquity for KELT-1. Finally, the vsin I{sub *} = 56 {+-} 2 km s{sup -1} of the primary is consistent at {approx}2{sigma} with tidal synchronization. Given the extreme parameters of the KELT-1 system, we expect it to provide an important testbed for theories of the emplacement and evolution of short-period companions, as well as theories of tidal dissipation and irradiated brown dwarf atmospheres.

  10. High performance flexible heat pipes

    NASA Technical Reports Server (NTRS)

    Shaubach, R. M.; Gernert, N. J.

    1985-01-01

    A Phase I SBIR NASA program for developing and demonstrating high-performance flexible heat pipes for use in the thermal management of spacecraft is examined. The program combines several technologies such as flexible screen arteries and high-performance circumferential distribution wicks within an envelope which is flexible in the adiabatic heat transport zone. The first six months of work during which the Phase I contract goal were met, are described. Consideration is given to the heat-pipe performance requirements. A preliminary evaluation shows that the power requirement for Phase II of the program is 30.5 kilowatt meters at an operating temperature from 0 to 100 C.

  11. High performance flexible heat pipes

    NASA Technical Reports Server (NTRS)

    Shaubach, R. M.; Gernert, N. J.

    1985-01-01

    A Phase I SBIR NASA program for developing and demonstrating high-performance flexible heat pipes for use in the thermal management of spacecraft is examined. The program combines several technologies such as flexible screen arteries and high-performance circumferential distribution wicks within an envelope which is flexible in the adiabatic heat transport zone. The first six months of work during which the Phase I contract goal were met, are described. Consideration is given to the heat-pipe performance requirements. A preliminary evaluation shows that the power requirement for Phase II of the program is 30.5 kilowatt meters at an operating temperature from 0 to 100 C.

  12. Phylogeography of E1b1b1b-M81 haplogroup and analysis of its subclades in Morocco.

    PubMed

    Reguig, Ahmed; Harich, Nourdin; Barakat, Abdelhamid; Rouba, Hassan

    2014-01-01

    In this study we analyzed 295 unrelated Berber-speaking men from northern, central, and southern Morocco to characterize frequency of the E1b1b1b-M81 haplogroup and to refine the phylogeny of its subclades: E1b1b1b1-M107, E1b1b1b2-M183, and E1b1b1b2a-M165. For this purpose, we typed four biallelic polymorphisms: M81, M107, M183, and M165. A large majority of the Berber-speaking male lineages belonged to the Y-chromosomal E1b1b1b-M81 haplogroup. The frequency ranged from 79.1% to 98.5% in all localities sampled. E1b1b1b2-M183 was the most dominant subclade in our samples, ranging from 65.1% to 83.1%. In contrast, the E1b1b1b1-M107 and E1b1b1b2a-M165 subclades were not found in our samples. Our results suggest a predominance of the E1b1b1b-M81 haplogroup among Moroccan Berber-speaking males with a decreasing gradient from south to north. The most prevalent subclade in this haplogroup was E1b1b1b2-M183, for which diffferences among these three groups were statistically significant between central and southern groups. Copyright © 2014 Wayne State University Press, Detroit, Michigan 48201-1309.

  13. Effects of the Novel High-affinity 5-HT(1B/1D)-receptor Ligand Frovatriptan on the Rat Carotid Artery.

    PubMed

    Saracheva, Kremena E; Prissadova, Natalia A; Turiiski, Valentin I; Slavchev, Valeri I; Krastev, Atanas D; Getova, Damianka P

    2017-03-01

    In blood vessels 5-HT stimulates sympathetic nerves, the endothelium and vascular smooth muscle cells. Triptans are specific anti-migraine drugs and they activate the serotoninergic 5HT1b/d receptors causing vasoconstriction of the cerebral vessels. To evaluate the effect of frovatriptan on isolated rat carotid artery. Contractile activity of the preparations was registered isometrically. Krebs solution (pH = 7.4) was used for washing smooth muscle (SM) preparations aerated with 95% O2 and 5% CO2 at 37°C. The 60-minute adaptation of tone level of preparations was taken as a starting tone and the changes such as contraction or relaxation were calculated using it. Frovatriptan (1×10-6 mol/l - 1×10-5 mol/l) induced a contraction, but at higher concentrations it caused relaxation of the carotid artery. The L-norepinephrine contractile reaction was enhanced in the presence of frovatriptan. In the presence of 5-HT2 receptor antagonist, methysergide, frovatriptan increased the relaxation. In the presence of the specific α-1 receptor antagonist, prazosin, the frovatriptan-induced relaxation decreased. The observed contractile effect of frovatriptan is probably associated with the main effect of the drug - activation of the serotoninergic 5HT1B /1D receptors causing vasoconstriction of the cerebral vessels and their anti-migraine effect. At higher concentrations, frovatriptan, most likely via some non-specific mechanism, could activate the following intracellular chain reaction: stimulation of α1D could activate eNOS which may increase in the concentration of NO which results in the final effect of relaxation.

  14. High performance dielectric materials development

    NASA Astrophysics Data System (ADS)

    Piche, Joe; Kirchner, Ted; Jayaraj, K.

    1994-09-01

    The mission of polymer composites materials technology is to develop materials and processing technology to meet DoD and commercial needs. The following are outlined in this presentation: high performance capacitors, high temperature aerospace insulation, rationale for choosing Foster-Miller (the reporting industry), the approach to the development and evaluation of high temperature insulation materials, and the requirements/evaluation parameters. Supporting tables and diagrams are included.

  15. High performance dielectric materials development

    NASA Technical Reports Server (NTRS)

    Piche, Joe; Kirchner, Ted; Jayaraj, K.

    1994-01-01

    The mission of polymer composites materials technology is to develop materials and processing technology to meet DoD and commercial needs. The following are outlined in this presentation: high performance capacitors, high temperature aerospace insulation, rationale for choosing Foster-Miller (the reporting industry), the approach to the development and evaluation of high temperature insulation materials, and the requirements/evaluation parameters. Supporting tables and diagrams are included.

  16. Rare MTNR1B variants impairing melatonin receptor 1B function contribute to type 2 diabetes

    PubMed Central

    Bonnefond, Amélie; Clément, Nathalie; Fawcett, Katherine; Yengo, Loïc; Vaillant, Emmanuel; Guillaume, Jean-Luc; Dechaume, Aurélie; Payne, Felicity; Roussel, Ronan; Czernichow, Sébastien; Hercberg, Serge; Hadjadj, Samy; Balkau, Beverley; Marre, Michel; Lantieri, Olivier; Langenberg, Claudia; Bouatia-Naji, Nabila; Charpentier, Guillaume; Vaxillaire, Martine; Rocheleau, Ghislain; Wareham, Nicholas J.; Sladek, Robert; McCarthy, Mark I.; Dina, Christian; Barroso, Inês; Jockers, Ralf; Froguel, Philippe

    2013-01-01

    Genome-wide association studies revealed that common non-coding variants in MTNR1B (encoding melatonin receptor 1B, also known as MT2) increase type 2 diabetes (T2D) risk1,2. Although the strongest association signal was highly significant (P<10−20), its contribution to T2D risk was modest (odds ratio, OR~1.10-1.15)1-3. We performed large-scale exon resequencing in 7,632 Europeans including 2,186 T2D patients and identified 40 non-synonymous variants, including 36 very rare variants (minor allele frequency, MAF<0.1%) associated with T2D (OR=3.31[1.78;6.18]95%); P=1.64×10−4. A four-tier functional investigation of all 40 mutants revealed that 14 were non-functional and rare (MAF<1%); four were very rare with complete loss of melatonin binding and signaling capabilities. Among the very rare variants, the partial or total loss-of-function variants, but not the neutral ones, contributed to T2D (OR=5.67[2.17;14.82]95%; P=4.09×10−4). Genotyping the four complete loss-of-function variants in 11,854 additional individuals revealed their association with T2D risk (Ncases=8,153/Ncontrols=10,100; OR=3.88[1.49;10.07]95%; P=5.37×10−3). This study establishes a firm functional link between MTNR1B and T2D risk. PMID:22286214

  17. INL High Performance Building Strategy

    SciTech Connect

    Jennifer D. Morton

    2010-02-01

    High performance buildings, also known as sustainable buildings and green buildings, are resource efficient structures that minimize the impact on the environment by using less energy and water, reduce solid waste and pollutants, and limit the depletion of natural resources while also providing a thermally and visually comfortable working environment that increases productivity for building occupants. As Idaho National Laboratory (INL) becomes the nation’s premier nuclear energy research laboratory, the physical infrastructure will be established to help accomplish this mission. This infrastructure, particularly the buildings, should incorporate high performance sustainable design features in order to be environmentally responsible and reflect an image of progressiveness and innovation to the public and prospective employees. Additionally, INL is a large consumer of energy that contributes to both carbon emissions and resource inefficiency. In the current climate of rising energy prices and political pressure for carbon reduction, this guide will help new construction project teams to design facilities that are sustainable and reduce energy costs, thereby reducing carbon emissions. With these concerns in mind, the recommendations described in the INL High Performance Building Strategy (previously called the INL Green Building Strategy) are intended to form the INL foundation for high performance building standards. This revised strategy incorporates the latest federal and DOE orders (Executive Order [EO] 13514, “Federal Leadership in Environmental, Energy, and Economic Performance” [2009], EO 13423, “Strengthening Federal Environmental, Energy, and Transportation Management” [2007], and DOE Order 430.2B, “Departmental Energy, Renewable Energy, and Transportation Management” [2008]), the latest guidelines, trends, and observations in high performance building construction, and the latest changes to the Leadership in Energy and Environmental Design

  18. High-Performance Thermoelectric Semiconductors

    NASA Technical Reports Server (NTRS)

    Fleurial, Jean-Pierre; Caillat, Thierry; Borshchevsky, Alexander

    1994-01-01

    Figures of merit almost double current state-of-art thermoelectric materials. IrSb3 is semiconductor found to exhibit exceptional thermoelectric properties. CoSb3 and RhSb3 have same skutterudite crystallographic structure as IrSb3, and exhibit exceptional transport properties expected to contribute to high thermoelectric performance. These three compounds form solid solutions. Combination of properties offers potential for development of new high-performance thermoelectric materials for more efficient thermoelectric power generators, coolers, and detectors.

  19. Effects of Al-5Ti-1B master alloy on the microstructural evaluation of a highly alloyed aluminum alloy produced by SIMA process

    SciTech Connect

    Alipour, M.; Emamy, M.; Azarbarmas, M.; Karamouz, M.

    2010-06-15

    This study was undertaken to investigate the influence of Al-5Ti-1B master alloy on the structural characteristics of Al-12Zn-3 Mg-2.5Cu aluminum alloy. The optimum amount of Ti containing master alloy for proper grain refining was selected as 6 wt.%. A modified strain-induced, melt-activated (SIMA) process for semi-solid processing of alloys was proposed. In order to examine the effectiveness of the modified SIMA process, the recrystallized microstructures of the Al alloy (Al-12Zn-3 Mg-2.5Cu) prepared by the modified SIMA processes were macroscopically. The modified SIMA process employed casting, warm multi-forging, recrystallization and partial melting instead of the conventional process. Reheating condition to obtain a fine globular microstructure was optimized. The microstructure evolution of reheated Al-12Zn-3 Mg-2.5Cu aluminum alloy was characterized by SEM (Scanning electron microscopy) and optical microscopy. In this study the relation between the induced strain with size and shape of grain size has been studied. Results indicated that with the increase of strain sphericity of particles, their size decreases and sphericity takes place in less reahiting time.

  20. Effects of Al—5Ti—1B master alloy on the microstructural evaluation of a highly alloyed aluminum alloy produced by SIMA process

    NASA Astrophysics Data System (ADS)

    Alipour, M.; Emamy, M.; Azarbarmas, M.; karamouz, M.

    2010-06-01

    This study was undertaken to investigate the influence of Al—5Ti—1B master alloy on the structural characteristics of Al—12Zn—3 Mg—2.5Cu aluminum alloy. The optimum amount of Ti containing master alloy for proper grain refining was selected as 6 wt.%. A modified strain-induced, melt-activated (SIMA) process for semi-solid processing of alloys was proposed. In order to examine the effectiveness of the modified SIMA process, the recrystallized microstructures of the Al alloy (Al—12Zn—3 Mg—2.5Cu) prepared by the modified SIMA processes were macroscopically. The modified SIMA process employed casting, warm multi-forging, recrystallization and partial melting instead of the conventional process. Reheating condition to obtain a fine globular microstructure was optimized. The microstructure evolution of reheated Al—12Zn—3 Mg—2.5Cu aluminum alloy was characterized by SEM (Scanning electron microscopy) and optical microscopy. In this study the relation between the induced strain with size and shape of grain size has been studied. Results indicated that with the increase of strain sphericity of particles, their size decreases and sphericity takes place in less reahiting time.

  1. High Performance Laser Package Design

    NASA Astrophysics Data System (ADS)

    Nelson, R. J.

    1987-01-01

    An optical coupling technique utilizing a high index lens to provide high coupling efficiencies (>75%) into single-mode fiber is described. This laser package design provides improved lateral tolerances (>1.5μm) over alternative designs. The improved tolerance provides for easier fiber alignment and assembly as well as excellent temperature stability. Data will be presented on coupling efficiency, lateral tolerance, temp-erature stability and laser performance characteristics.

  2. High performance rolling element bearing

    NASA Technical Reports Server (NTRS)

    Bursey, Jr., Roger W. (Inventor); Olinger, Jr., John B. (Inventor); Owen, Samuel S. (Inventor); Poole, William E. (Inventor); Haluck, David A. (Inventor)

    1993-01-01

    A high performance rolling element bearing (5) which is particularly suitable for use in a cryogenically cooled environment, comprises a composite cage (45) formed from glass fibers disposed in a solid lubricant matrix of a fluorocarbon polymer. The cage includes inserts (50) formed from a mixture of a soft metal and a solid lubricant such as a fluorocarbon polymer.

  3. High Performance Bulk Thermoelectric Materials

    SciTech Connect

    Ren, Zhifeng

    2013-03-31

    Over 13 plus years, we have carried out research on electron pairing symmetry of superconductors, growth and their field emission property studies on carbon nanotubes and semiconducting nanowires, high performance thermoelectric materials and other interesting materials. As a result of the research, we have published 104 papers, have educated six undergraduate students, twenty graduate students, nine postdocs, nine visitors, and one technician.

  4. High-Performance Polymeric Materials.

    DTIC Science & Technology

    1987-12-07

    interactions, Chain packing, Polybenzobisoxazoles Electrical conductivity Polybenzobisthiazoles Ceramic particles Chain flexibility Elastomer reinforcement...structures for the polybenzobisoxazole (PBO) and polybenzobisthiazole (PBT) chains originally synthesized and much studied because of their utility as...high-performance fibers and films. For cts-PBO, trans-PBO. and trans-PBT chains in their coplanar conformations, the band gaps in the axial direction

  5. High performance bilateral telerobot control.

    PubMed

    Kline-Schoder, Robert; Finger, William; Hogan, Neville

    2002-01-01

    Telerobotic systems are used when the environment that requires manipulation is not easily accessible to humans, as in space, remote, hazardous, or microscopic applications or to extend the capabilities of an operator by scaling motions and forces. The Creare control algorithm and software is an enabling technology that makes possible guaranteed stability and high performance for force-feedback telerobots. We have developed the necessary theory, structure, and software design required to implement high performance telerobot systems with time delay. This includes controllers for the master and slave manipulators, the manipulator servo levels, the communication link, and impedance shaping modules. We verified the performance using both bench top hardware as well as a commercial microsurgery system.

  6. Developing high-performance leaders.

    PubMed

    Melum, Mara

    2002-01-01

    Although there is widespread recognition that strong leadership is key in these challenging times, many companies provide only the tip of the iceberg of leadership development support. This article is a resource for high-powered leadership development systems that will have an impact on performance. Four topics are discussed: (1) models, (2) investment and results, (3) critical success factors, and (4) case studies of how the 3M Company and HealthPartners develop high-performance leaders. Studies that quantity the effect of leadership development on performance are noted. Five critical success factors are described, and examples from leadership development benchmark organizations such as General Electric and Reell Precision Manufacturing are discussed.

  7. High Brain Ammonia Tolerance and Down-Regulation of Na+:K+:2Cl- Cotransporter 1b mRNA and Protein Expression in the Brain of the Swamp Eel, Monopterus albus, Exposed to Environmental Ammonia or Terrestrial Conditions

    PubMed Central

    Ip, Yuen K.; Hou, Zhisheng; Chen, Xiu L.; Ong, Jasmine L. Y.; Chng, You R.; Ching, Biyun; Hiong, Kum C.; Chew, Shit F.

    2013-01-01

    Na+:K+:2Cl- cotransporter 1 (NKCC1) has been implicated in mediating ischemia-, trauma- or ammonia-induced astrocyte swelling/brain edema in mammals. This study aimed to determine the effects of ammonia or terrestrial exposure on ammonia concentrations in the plasma and brain, and the mRNA expression and protein abundance of nkcc/Nkcc in the brain, of the swamp eel Monopterusalbus. Ammonia exposure led to a greater increase in the ammonia concentration in the brain of M. albus than terrestrial exposure. The brain ammonia concentration of M. albus reached 4.5 µmol g-1 and 2.7 µmol g-1 after 6 days of exposure to 50 mmol l-1 NH4Cl and terrestrial conditions, respectively. The full cDNA coding sequence of nkcc1b from M. albus brain comprised 3276 bp and coded for 1092 amino acids with an estimated molecular mass of 119.6 kDa. A molecular characterization indicated that it could be activated through phosphorylation and/or glycosylation by osmotic and/or oxidative stresses. Ammonia exposure for 1 day or 6 days led to significant decreases in the nkcc1b mRNA expression and Nkcc1b protein abundance in the brain of M. albus. In comparison, a significant decrease in nkcc1b mRNA expression was observed in the brain of M. albus only after 6 days of terrestrial exposure, but both 1 day and 6 days of terrestrial exposure resulted in significant decreases in the protein abundance of Nkcc1b. These results are novel because it has been established in mammals that ammonia up-regulates NKCC1 expression in astrocytes and NKCC1 plays an important role in ammonia-induced astrocyte swelling and brain edema. By contrast, our results indicate for the first time that M. albus is able to down-regulate the mRNA and protein expression of nkcc1b/Nkcc1b in the brain when confronted with ammonia toxicity, which could be one of the contributing factors to its extraordinarily high brain ammonia tolerance. PMID:24069137

  8. High brain ammonia tolerance and down-regulation of Na+:K+:2Cl(-) Cotransporter 1b mRNA and protein expression in the brain of the Swamp Eel, Monopterus albus, exposed to environmental ammonia or terrestrial conditions.

    PubMed

    Ip, Yuen K; Hou, Zhisheng; Chen, Xiu L; Ong, Jasmine L Y; Chng, You R; Ching, Biyun; Hiong, Kum C; Chew, Shit F

    2013-01-01

    Na(+):K(+):2Cl(-) cotransporter 1 (NKCC1) has been implicated in mediating ischemia-, trauma- or ammonia-induced astrocyte swelling/brain edema in mammals. This study aimed to determine the effects of ammonia or terrestrial exposure on ammonia concentrations in the plasma and brain, and the mRNA expression and protein abundance of nkcc/Nkcc in the brain, of the swamp eel Monopterusalbus. Ammonia exposure led to a greater increase in the ammonia concentration in the brain of M. albus than terrestrial exposure. The brain ammonia concentration of M. albus reached 4.5 µmol g(-1) and 2.7 µmol g(-1) after 6 days of exposure to 50 mmol l(-1) NH4Cl and terrestrial conditions, respectively. The full cDNA coding sequence of nkcc1b from M. albus brain comprised 3276 bp and coded for 1092 amino acids with an estimated molecular mass of 119.6 kDa. A molecular characterization indicated that it could be activated through phosphorylation and/or glycosylation by osmotic and/or oxidative stresses. Ammonia exposure for 1 day or 6 days led to significant decreases in the nkcc1b mRNA expression and Nkcc1b protein abundance in the brain of M. albus. In comparison, a significant decrease in nkcc1b mRNA expression was observed in the brain of M. albus only after 6 days of terrestrial exposure, but both 1 day and 6 days of terrestrial exposure resulted in significant decreases in the protein abundance of Nkcc1b. These results are novel because it has been established in mammals that ammonia up-regulates NKCC1 expression in astrocytes and NKCC1 plays an important role in ammonia-induced astrocyte swelling and brain edema. By contrast, our results indicate for the first time that M. albus is able to down-regulate the mRNA and protein expression of nkcc1b/Nkcc1b in the brain when confronted with ammonia toxicity, which could be one of the contributing factors to its extraordinarily high brain ammonia tolerance.

  9. High Performance Tools And Technologies

    SciTech Connect

    Collette, M R; Corey, I R; Johnson, J R

    2005-01-24

    This goal of this project was to evaluate the capability and limits of current scientific simulation development tools and technologies with specific focus on their suitability for use with the next generation of scientific parallel applications and High Performance Computing (HPC) platforms. The opinions expressed in this document are those of the authors, and reflect the authors' current understanding and functionality of the many tools investigated. As a deliverable for this effort, we are presenting this report describing our findings along with an associated spreadsheet outlining current capabilities and characteristics of leading and emerging tools in the high performance computing arena. This first chapter summarizes our findings (which are detailed in the other chapters) and presents our conclusions, remarks, and anticipations for the future. In the second chapter, we detail how various teams in our local high performance community utilize HPC tools and technologies, and mention some common concerns they have about them. In the third chapter, we review the platforms currently or potentially available to utilize these tools and technologies on to help in software development. Subsequent chapters attempt to provide an exhaustive overview of the available parallel software development tools and technologies, including their strong and weak points and future concerns. We categorize them as debuggers, memory checkers, performance analysis tools, communication libraries, data visualization programs, and other parallel development aides. The last chapter contains our closing information. Included with this paper at the end is a table of the discussed development tools and their operational environment.

  10. High performance pyroelectric infrared detector

    NASA Astrophysics Data System (ADS)

    Hu, Xu; Luo, Haosu; Ji, Yulong; Yang, Chunli

    2015-10-01

    Single infrared detector made with Relaxative ferroelectric crystal(PMNT) present excellence performance. In this paper include detector capacitance, characteristic of frequency--response, characteristic of detectivity. The measure result show that detectivity of detector made with relaxative ferroelectric crystal(PMNT) exceed three times than made with LT, the D*achieved than 1*109cmHz0.5W-1. The detector will be applied on NDIR spectrograph, FFT spectrograph and so on. The high performance pyroelectric infrared detector be developed that will be broadened application area of infrared detector.

  11. Sporadic pseudohypoparathyroidism type-1b with asymptomatic hypocalcemia.

    PubMed

    Goto, Motohide; Yamamoto, Yukiyo; Ishii, Masahiro; Nakamura, Akie; Sano, Shinichiro; Kagami, Masayo; Fukami, Maki; Saito, Reiko; Araki, Shunsuke; Kubo, Kazuyasu; Kawagoe, Rinko; Kawada, Yasusada; Kusuhara, Koichi

    2016-11-01

    Pseudohypoparathyroidism type 1b (PHP-1b) is usually diagnosed on various symptoms of hypocalcemia. Previous studies reported a few cases of autosomal dominant pattern PHP-1b identified on familial analysis with asymptomatic hypocalcemia. Herein we report the case of a 6-year-old male patient with sporadic PHP-1b incidentally detected on preoperative examination. He had neither characteristic findings of Albright hereditary osteodystrophy nor evidence of tetany. Sporadic PHP-1b was diagnosed on the basis of clinical observation and laboratory examination. In addition, genetic testing using methylation-specific multiplex ligation-dependent probe amplification indicated broad methylation abnormalities and confirmed the sporadic form of PHP-1b. Sporadic PHP-1b might often be overlooked when diagnosis is done simply on definitive clinical features. To avoid this, DNA sequencing and methylation analysis should be performed even in the absence of definitive clinical features. © 2016 Japan Pediatric Society.

  12. Toward high performance graphene fibers.

    PubMed

    Chen, Li; He, Yuling; Chai, Songgang; Qiang, Hong; Chen, Feng; Fu, Qiang

    2013-07-07

    Two-dimensional graphene and graphene-based materials have attracted tremendous interest, hence much attention has been drawn to exploring and applying their exceptional characteristics and properties. Integration of graphene sheets into macroscopic fibers is a very important way for their application and has received increasing interest. In this study, neat and macroscopic graphene fibers were continuously spun from graphene oxide (GO) suspensions followed by chemical reduction. By varying wet-spinning conditions, a series of graphene fibers were prepared, then, the structural features, mechanical and electrical performances of the fibers were investigated. We found the orientation of graphene sheets, the interaction between inter-fiber graphene sheets and the defects in the fibers have a pronounced effect on the properties of the fibers. Graphene fibers with excellent mechanical and electrical properties will yield great advances in high-tech applications. These findings provide guidance for the future production of high performance graphene fibers.

  13. High-performance sports medicine.

    PubMed

    Speed, Cathy

    2013-02-01

    High performance sports medicine involves the medical care of athletes, who are extraordinary individuals and who are exposed to intensive physical and psychological stresses during training and competition. The physician has a broad remit and acts as a 'medical guardian' to optimise health while minimising risks. This review describes this interesting field of medicine, its unique challenges and priorities for the physician in delivering best healthcare.

  14. New, high performance rotating parachute

    SciTech Connect

    Pepper, W.B. Jr.

    1983-01-01

    A new rotating parachute has been designed primarily for recovery of high performance reentry vehicles. Design and development/testing results are presented from low-speed wind tunnel testing, free-flight deployments at transonic speeds and tests in a supersonic wind tunnel at Mach 2.0. Drag coefficients of 1.15 based on the 2-ft diameter of the rotor have been measured in the wind tunnel. Stability of the rotor is excellent.

  15. High performance ammonium nitrate propellant

    NASA Technical Reports Server (NTRS)

    Anderson, F. A. (Inventor)

    1979-01-01

    A high performance propellant having greatly reduced hydrogen chloride emission is presented. It is comprised of: (1) a minor amount of hydrocarbon binder (10-15%), (2) at least 85% solids including ammonium nitrate as the primary oxidizer (about 40% to 70%), (3) a significant amount (5-25%) powdered metal fuel, such as aluminum, (4) a small amount (5-25%) of ammonium perchlorate as a supplementary oxidizer, and (5) optionally a small amount (0-20%) of a nitramine.

  16. Reduced Toxicity High Performance Monopropellant

    DTIC Science & Technology

    2011-09-01

    distribution unlimited Propellant Performance Characteristics LMP - 103S AF-M315E Hydrazine Flame Temperature 1600ºC 1900ºC 600 oC Isp 252 (theor)235 sec...public release; distribution unlimited Compatibility and Handling Propellant LMP - 103S AF-M315E Thruster Materials Compatibility High combustion...detonation Bikini gauges indicate > 103 kPa @ 50ft Fragments thrown > 185 m Punched hole in end cap 12 Distribution A: Approved for public

  17. High-performance permanent magnets

    NASA Astrophysics Data System (ADS)

    Goll, D.; Kronmüller, H.

    High-performance permanent magnets (pms) are based on compounds with outstanding intrinsic magnetic properties as well as on optimized microstructures and alloy compositions. The most powerful pm materials at present are RE-TM intermetallic alloys which derive their exceptional magnetic properties from the favourable combination of rare earth metals (RE=Nd, Pr, Sm) with transition metals (TM=Fe, Co), in particular magnets based on (Nd,Pr)2Fe14B and Sm2(Co,Cu,Fe,Zr)17. Their development during the last 20 years has involved a dramatic improvement in their performance by a factor of >15 compared with conventional ferrite pms therefore contributing positively to the ever-increasing demand for pms in many (including new) application fields, to the extent that RE-TM pms now account for nearly half of the worldwide market. This review article first gives a brief introduction to the basics of ferromagnetism to confer an insight into the variety of (permanent) magnets, their manufacture and application fields. We then examine the rather complex relationship between the microstructure and the magnetic properties for the two highest-performance and most promising pm materials mentioned. By using numerical micromagnetic simulations on the basis of the Finite Element technique the correlation can be quantitatively predicted, thus providing a powerful tool for the further development of optimized high-performance pms.

  18. High-performance permanent magnets.

    PubMed

    Goll, D; Kronmüller, H

    2000-10-01

    High-performance permanent magnets (pms) are based on compounds with outstanding intrinsic magnetic properties as well as on optimized microstructures and alloy compositions. The most powerful pm materials at present are RE-TM intermetallic alloys which derive their exceptional magnetic properties from the favourable combination of rare earth metals (RE = Nd, Pr, Sm) with transition metals (TM = Fe, Co), in particular magnets based on (Nd.Pr)2Fe14B and Sm2(Co,Cu,Fe,Zr)17. Their development during the last 20 years has involved a dramatic improvement in their performance by a factor of > 15 compared with conventional ferrite pms therefore contributing positively to the ever-increasing demand for pms in many (including new) application fields, to the extent that RE-TM pms now account for nearly half of the worldwide market. This review article first gives a brief introduction to the basics of ferromagnetism to confer an insight into the variety of (permanent) magnets, their manufacture and application fields. We then examine the rather complex relationship between the microstructure and the magnetic properties for the two highest-performance and most promising pm materials mentioned. By using numerical micromagnetic simulations on the basis of the Finite Element technique the correlation can be quantitatively predicted, thus providing a powerful tool for the further development of optimized high-performance pms.

  19. High Performance Parallel Computational Nanotechnology

    NASA Technical Reports Server (NTRS)

    Saini, Subhash; Craw, James M. (Technical Monitor)

    1995-01-01

    At a recent press conference, NASA Administrator Dan Goldin encouraged NASA Ames Research Center to take a lead role in promoting research and development of advanced, high-performance computer technology, including nanotechnology. Manufacturers of leading-edge microprocessors currently perform large-scale simulations in the design and verification of semiconductor devices and microprocessors. Recently, the need for this intensive simulation and modeling analysis has greatly increased, due in part to the ever-increasing complexity of these devices, as well as the lessons of experiences such as the Pentium fiasco. Simulation, modeling, testing, and validation will be even more important for designing molecular computers because of the complex specification of millions of atoms, thousands of assembly steps, as well as the simulation and modeling needed to ensure reliable, robust and efficient fabrication of the molecular devices. The software for this capacity does not exist today, but it can be extrapolated from the software currently used in molecular modeling for other applications: semi-empirical methods, ab initio methods, self-consistent field methods, Hartree-Fock methods, molecular mechanics; and simulation methods for diamondoid structures. In as much as it seems clear that the application of such methods in nanotechnology will require powerful, highly powerful systems, this talk will discuss techniques and issues for performing these types of computations on parallel systems. We will describe system design issues (memory, I/O, mass storage, operating system requirements, special user interface issues, interconnects, bandwidths, and programming languages) involved in parallel methods for scalable classical, semiclassical, quantum, molecular mechanics, and continuum models; molecular nanotechnology computer-aided designs (NanoCAD) techniques; visualization using virtual reality techniques of structural models and assembly sequences; software required to

  20. High Performance Parallel Computational Nanotechnology

    NASA Technical Reports Server (NTRS)

    Saini, Subhash; Craw, James M. (Technical Monitor)

    1995-01-01

    At a recent press conference, NASA Administrator Dan Goldin encouraged NASA Ames Research Center to take a lead role in promoting research and development of advanced, high-performance computer technology, including nanotechnology. Manufacturers of leading-edge microprocessors currently perform large-scale simulations in the design and verification of semiconductor devices and microprocessors. Recently, the need for this intensive simulation and modeling analysis has greatly increased, due in part to the ever-increasing complexity of these devices, as well as the lessons of experiences such as the Pentium fiasco. Simulation, modeling, testing, and validation will be even more important for designing molecular computers because of the complex specification of millions of atoms, thousands of assembly steps, as well as the simulation and modeling needed to ensure reliable, robust and efficient fabrication of the molecular devices. The software for this capacity does not exist today, but it can be extrapolated from the software currently used in molecular modeling for other applications: semi-empirical methods, ab initio methods, self-consistent field methods, Hartree-Fock methods, molecular mechanics; and simulation methods for diamondoid structures. In as much as it seems clear that the application of such methods in nanotechnology will require powerful, highly powerful systems, this talk will discuss techniques and issues for performing these types of computations on parallel systems. We will describe system design issues (memory, I/O, mass storage, operating system requirements, special user interface issues, interconnects, bandwidths, and programming languages) involved in parallel methods for scalable classical, semiclassical, quantum, molecular mechanics, and continuum models; molecular nanotechnology computer-aided designs (NanoCAD) techniques; visualization using virtual reality techniques of structural models and assembly sequences; software required to

  1. Selectivity and potency of microcystin congeners against OATP1B1 and OATP1B3 expressing cancer cells.

    PubMed

    Niedermeyer, Timo H J; Daily, Abigail; Swiatecka-Hagenbruch, Monika; Moscow, Jeffrey A

    2014-01-01

    Microcystins are potent phosphatase inhibitors and cellular toxins. They require active transport by OATP1B1 and OATP1B3 transporters for uptake into human cells, and the high expression of these transporters in the liver accounts for their selective hepatic toxicity. Several human tumors have been shown to have high levels of expression of OATP1B3 but not OATP1B1, the main transporter in liver cells. We hypothesized that microcystin variants could be isolated that are transported preferentially by OATP1B3 relative to OATP1B1 to advance as anticancer agents with clinically tolerable hepatic toxicity. Microcystin variants have been isolated and tested for cytotoxicity in cancer cells stably transfected with OATP1B1 and OATP1B3 transporters. Microcystin variants with cytotoxic OATP1B1/OATP1B3 IC50 ratios that ranged between 0.2 and 32 were found, representing a 150-fold range in transporter selectivity. As microcystin structure has a significant impact on transporter selectivity, it is potentially possible to develop analogs with even more pronounced OATP1B3 selectivity and thus enable their development as anticancer drugs.

  2. High Performance Pulse Tube Cryocoolers

    NASA Astrophysics Data System (ADS)

    Olson, J. R.; Roth, E.; Champagne, P.; Evtimov, B.; Nast, T. C.

    2008-03-01

    Lockheed Martin's Advanced Technology Center has been developing pulse tube cryocoolers for more than ten years. Recent innovations include successful testing of four-stage coldheads, no-load temperature below 4 K, and the recent development of a high-efficiency compressor. This paper discusses the predicted performance of single and multiple stage pulse tube coldheads driven by our new 6 kg "M5Midi" compressor, which is capable of 90% efficiency with 200 W input power, and a maximum input power of 1000 W. This compressor retains the simplicity of earlier LM-ATC compressors: it has a moving magnet and an external electrical coil, minimizing organics in the working gas and requiring no electrical penetrations through the pressure wall. Motor losses were minimized during design, resulting in a simple, easily-manufactured compressor with state-of-the-art motor efficiency. The predicted cryocooler performance is presented as simple formulae, allowing an engineer to include the impact of a highly-optimized cryocooler into a full system analysis. Performance is given as a function of the heat rejection temperature and the cold tip temperatures and cooling loads.

  3. High performance aerated lagoon systems

    SciTech Connect

    Rich, L.

    1999-08-01

    At a time when less money is available for wastewater treatment facilities and there is increased competition for the local tax dollar, regulatory agencies are enforcing stricter effluent limits on treatment discharges. A solution for both municipalities and industry is to use aerated lagoon systems designed to meet these limits. This monograph, prepared by a recognized expert in the field, provides methods for the rational design of a wide variety of high-performance aerated lagoon systems. Such systems range from those that can be depended upon to meet secondary treatment standards alone to those that, with the inclusion of intermittent sand filters or elements of sequenced biological reactor (SBR) technology, can also provide for nitrification and nutrient removal. Considerable emphasis is placed on the use of appropriate performance parameters, and an entire chapter is devoted to diagnosing performance failures. Contents include: principles of microbiological processes, control of algae, benthal stabilization, design for CBOD removal, design for nitrification and denitrification in suspended-growth systems, design for nitrification in attached-growth systems, phosphorus removal, diagnosing performance.

  4. HIGH PERFORMANCE EBIS FOR RHIC.

    SciTech Connect

    ALESSI,J.; BEEBE, E.; GOULD, O.; KPONOU, A.; LOCKEY, R.; PIKIN, A.; RAPARIA, D.; RITTER, J.; SNYDSTRUP, L.

    2007-06-25

    An Electron Beam Ion Source (EBIS), capable of producing high charge states and high beam currents of any heavy ion species in short pulses, is ideally suited for injection into a synchrotron. An EBIS-based, high current, heavy ion preinjector is now being built at Brookhaven to provide increased capabilities for the Relativistic Heavy Ion Collider (RHIC), and the NASA Space Radiation Laboratory (NSRL). Benefits of the new preinjector include the ability to produce ions of any species, fast switching between species to serve the simultaneous needs of multiple programs, and lower operating and maintenance costs. A state-of-the-art EBIS, operating with an electron beam current of up to 10 A, and producing multi-milliamperes of high charge state heavy ions, has been developed at Brookhaven, and has been operating very successfully on a test bench for several years. The present performance of this high-current EBIS is presented, along with details of the design of the scaled-up EBIS for RHIC, and the status of its construction. Other aspects of the project, including design and construction of the heavy ion RFQ, Linac, and matching beamlines, are also mentioned.

  5. High Performance Proactive Digital Forensics

    NASA Astrophysics Data System (ADS)

    Alharbi, Soltan; Moa, Belaid; Weber-Jahnke, Jens; Traore, Issa

    2012-10-01

    With the increase in the number of digital crimes and in their sophistication, High Performance Computing (HPC) is becoming a must in Digital Forensics (DF). According to the FBI annual report, the size of data processed during the 2010 fiscal year reached 3,086 TB (compared to 2,334 TB in 2009) and the number of agencies that requested Regional Computer Forensics Laboratory assistance increasing from 689 in 2009 to 722 in 2010. Since most investigation tools are both I/O and CPU bound, the next-generation DF tools are required to be distributed and offer HPC capabilities. The need for HPC is even more evident in investigating crimes on clouds or when proactive DF analysis and on-site investigation, requiring semi-real time processing, are performed. Although overcoming the performance challenge is a major goal in DF, as far as we know, there is almost no research on HPC-DF except for few papers. As such, in this work, we extend our work on the need of a proactive system and present a high performance automated proactive digital forensic system. The most expensive phase of the system, namely proactive analysis and detection, uses a parallel extension of the iterative z algorithm. It also implements new parallel information-based outlier detection algorithms to proactively and forensically handle suspicious activities. To analyse a large number of targets and events and continuously do so (to capture the dynamics of the system), we rely on a multi-resolution approach to explore the digital forensic space. Data set from the Honeynet Forensic Challenge in 2001 is used to evaluate the system from DF and HPC perspectives.

  6. Contribution of OATP1B1 and OATP1B3 to the Disposition of Sorafenib and Sorafenib-Glucuronide

    PubMed Central

    Zimmerman, Eric I.; Hu, Shuiying; Roberts, Justin L.; Gibson, Alice A.; Orwick, Shelley J.; Li, Lie; Sparreboom, Alex; Baker, Sharyn D.

    2013-01-01

    Purpose Many tyrosine kinase inhibitors (TKIs) undergo extensive hepatic metabolism, but mechanisms of their hepatocellular uptake remain poorly understood. We hypothesized that liver uptake of TKIs is mediated by the solute carriers OATP1B1 and OATP1B3. Experimental Design Transport of crizotinib, dasatinib, gefitinib, imatinib, nilotinib, pazopanib, sorafenib, sunitinib, vandetanib, and vemurafenib was studied in vitro using artificial membranes (PAMPA) and HEK293 cell lines stably transfected with OATP1B1, OATP1B3, or the ortholog mouse transporter, Oatp1b2. Pharmacokinetic studies were performed with Oatp1b2-knockout mice and humanized OATP1B1- or OATP1B3-transgenic mice. Results All 10 TKIs were identified as substrates of OATP1B1, OATP1B3, or both. Transport of sorafenib was investigated further, since its diffusion was particularly low in the PAMPA assay (<4%) compared to other TKIs that were transported by both OATP1B1 and OATP1B3. While Oatp1b2 deficiency in vivo had minimal influence on parent and active metabolite N-oxide drug exposure, plasma levels of the glucuronic-acid metabolite of sorafenib (sorafenib-glucuronide) were increased >8-fold in Oatp1b2-knockout mice. This finding was unrelated to possible changes in intrinsic metabolic capacity for sorafenib-glucuronide formation in hepatic or intestinal microsomes ex vivo. Ensuing experiments revealed that sorafenib-glucuronide was itself a transported substrate of Oatp1b2 (17.5-fold vs control), OATP1B1 (10.6-fold), and OATP1B3 (6.4-fold), and introduction of the human transporters in Oatp1b2-knockout mice provided partial restoration of function. Conclusions These findings signify a unique role for OATP1B1 and OATP1B3 in the elimination of sorafenib-glucuronide, and suggest a role for these transporters in the in vivo handling of glucuronic acid conjugates of drugs. PMID:23340295

  7. High rates of glucose utilization in the gas gland of Atlantic cod (Gadus morhua) are supported by GLUT1 and HK1b.

    PubMed

    Clow, Kathy A; Short, Connie E; Hall, Jennifer R; Gendron, Robert L; Paradis, Hélène; Ralhan, Ankur; Driedzic, William R

    2016-09-01

    The gas gland of physoclistous fish utilizes glucose to generate lactic acid that leads to the off-loading of oxygen from haemoglobin. This study addresses characteristics of the first two steps in glucose utilization in the gas gland of Atlantic cod (Gadus morhua). Glucose metabolism by isolated gas gland cells was 12- and 170-fold higher, respectively, than that in heart and red blood cells (RBCs) as determined by the production of (3)H2O from [2-(3)H]glucose. In the gas gland, essentially all of the glucose consumed was converted to lactate. Glucose uptake in the gas gland shows a very high dependence upon facilitated transport as evidenced by saturation of uptake of 2-deoxyglucose at a low extracellular concentration and a requirement for high levels of cytochalasin B for uptake inhibition despite the high efficacy of this treatment in heart and RBCs. Glucose transport is via glucose transporter 1 (GLUT1), which is localized to the glandular cells. GLUT1 western blot analysis from whole-tissue lysates displayed a band with a relative molecular mass of 52 kDa, consistent with the deduced amino acid sequence. Levels of 52 kDa GLUT1 in the gas gland were 2.3- and 33-fold higher, respectively, than those in heart and RBCs, respectively. Glucose phosphorylation is catalysed by hexokinase Ib (HKIb), a paralogue that cannot bind to the outer mitochondrial membrane. Transcript levels of HKIb in the gas gland were 52- and 57-fold more abundant, respectively, than those in heart and RBCs. It appears that high levels of GLUT1 protein and an unusual isoform of HKI are both critical for the high rates of glycolysis in gas gland cells. © 2016. Published by The Company of Biologists Ltd.

  8. Phase 1b study of new posaconazole tablet for prevention of invasive fungal infections in high-risk patients with neutropenia.

    PubMed

    Duarte, Rafael F; López-Jiménez, Javier; Cornely, Oliver A; Laverdiere, Michel; Helfgott, David; Haider, Shariq; Chandrasekar, Pranatharthi; Langston, Amelia; Perfect, John; Ma, Lei; van Iersel, Marlou L P S; Connelly, Nancy; Kartsonis, Nicholas; Waskin, Hetty

    2014-10-01

    Posaconazole tablets, a new oral formulation of posaconazole, can be effective when given as antifungal prophylaxis to neutropenic patients at high risk for invasive fungal infection (e.g., those with acute myelogenous leukemia or myelodysplastic syndrome). Such effectiveness might be specifically important to patients with poor oral intake because of nausea, vomiting, or chemotherapy-associated mucositis. This was a prospective, global study in high-risk patients to characterize the pharmacokinetics and safety profile of posaconazole tablets and to identify the dose of posaconazole tablets that would provide exposure within a predefined range of exposures (steady-state average concentration [area under the concentration-time curve/24 h] of ≥500 ng/ml and ≤2,500 ng/ml in >90% of patients). The study evaluated two sequential dosing cohorts: 200 mg posaconazole once daily (n = 20) and 300 mg posaconazole once daily (n = 34) (both cohorts had a twice-daily loading dose on day 1) taken without regard to food intake during the neutropenic period for ≤28 days. The exposure target was reached (day 8) in 15 of 19 (79%) pharmacokinetic-evaluable patients taking 200 mg posaconazole once daily and in 31 of 32 (97%) patients taking 300 mg posaconazole once daily; 300 mg posaconazole once daily achieved the desired exposure target. Posaconazole tablets were generally well tolerated in high-risk neutropenic patients. (This study has been registered at ClinicalTrials.gov under registration no. NCT01777763.).

  9. The High Performance Storage System

    SciTech Connect

    Coyne, R.A.; Hulen, H.; Watson, R.

    1993-09-01

    The National Storage Laboratory (NSL) was organized to develop, demonstrate and commercialize technology for the storage system that will be the future repositories for our national information assets. Within the NSL four Department of Energy laboratories and IBM Federal System Company have pooled their resources to develop an entirely new High Performance Storage System (HPSS). The HPSS project concentrates on scalable parallel storage system for highly parallel computers as well as traditional supercomputers and workstation clusters. Concentrating on meeting the high end of storage system and data management requirements, HPSS is designed using network-connected storage devices to transfer data at rates of 100 million bytes per second and beyond. The resulting products will be portable to many vendor`s platforms. The three year project is targeted to be complete in 1995. This paper provides an overview of the requirements, design issues, and architecture of HPSS, as well as a description of the distributed, multi-organization industry and national laboratory HPSS project.

  10. High performance solar Stirling system

    NASA Technical Reports Server (NTRS)

    Stearns, J. W.; Haglund, R.

    1981-01-01

    A full-scale Dish-Stirling system experiment, at a power level of 25 kWe, has been tested during 1981 on the Test Bed Concentrator No. 2 at the Parabolic Dish Test Site, Edwards, CA. Test components, designed and developed primarily by industrial contractors for the Department of Energy, include an advanced Stirling engine driving an induction alternator, a directly-coupled solar receiver with a natural gas combustor for hybrid operation and a breadboard control system based on a programmable controller and standard utility substation components. The experiment demonstrated practicality of the solar Stirling application and high system performance into a utility grid. This paper describes the design and its functions, and the test results obtained.

  11. FPGA Based High Performance Computing

    SciTech Connect

    Bennett, Dave; Mason, Jeff; Sundararajan, Prasanna; Dellinger, Erik; Putnam, Andrew; Storaasli, Olaf O

    2008-01-01

    Current high performance computing (HPC) applications are found in many consumer, industrial and research fields. From web searches to auto crash simulations to weather predictions, these applications require large amounts of power by the compute farms and supercomputers required to run them. The demand for more and faster computation continues to increase along with an even sharper increase in the cost of the power required to operate and cool these installations. The ability of standard processor based systems to address these needs has declined in both speed of computation and in power consumption over the past few years. This paper presents a new method of computation based upon programmable logic as represented by Field Programmable Gate Arrays (FPGAs) that addresses these needs in a manner requiring only minimal changes to the current software design environment.

  12. High performance solar Stirling system

    NASA Astrophysics Data System (ADS)

    Stearns, J. W.; Haglund, R.

    1981-12-01

    A full-scale Dish-Stirling system experiment, at a power level of 25 kWe, has been tested during 1981 on the Test Bed Concentrator No. 2 at the Parabolic Dish Test Site, Edwards, CA. Test components, designed and developed primarily by industrial contractors for the Department of Energy, include an advanced Stirling engine driving an induction alternator, a directly-coupled solar receiver with a natural gas combustor for hybrid operation and a breadboard control system based on a programmable controller and standard utility substation components. The experiment demonstrated practicality of the solar Stirling application and high system performance into a utility grid. This paper describes the design and its functions, and the test results obtained.

  13. High performance phenolic pultrusion resin

    SciTech Connect

    Qureshi, S.P.; Ingram, W.H.; Smith, C.

    1996-11-01

    Today, Phenol-Formaldehyde (PF) resins are the materials of choice for aerospace interior applications, primarily due to low FST (flame, smoke and toxicity). Since 1990, growth of PF resins has been steadily increasing in non-aerospace applications (which include mass transit, construction, marine, mine ducting and offshore oil) due to low FST and reasonable cost. This paper describes one component phenol-formaldehyde resin that was jointly developed with Morrison Molded Fiber Glass for their pultrusion process. Physical properties of the resin with flame/smoke/toxicity, chemical resistance and mechanical performance of the pultruded RP are discussed. Neat resin screening tests to identify high-temperature formulations are explored. Research continues at Georgia-Pacific to investigate the effect of formulation variables on processing and mechanical properties.

  14. High Performance Perovskite Solar Cells.

    PubMed

    Tong, Xin; Lin, Feng; Wu, Jiang; Wang, Zhiming M

    2016-05-01

    Perovskite solar cells fabricated from organometal halide light harvesters have captured significant attention due to their tremendously low device costs as well as unprecedented rapid progress on power conversion efficiency (PCE). A certified PCE of 20.1% was achieved in late 2014 following the first study of long-term stable all-solid-state perovskite solar cell with a PCE of 9.7% in 2012, showing their promising potential towards future cost-effective and high performance solar cells. Here, notable achievements of primary device configuration involving perovskite layer, hole-transporting materials (HTMs) and electron-transporting materials (ETMs) are reviewed. Numerous strategies for enhancing photovoltaic parameters of perovskite solar cells, including morphology and crystallization control of perovskite layer, HTMs design and ETMs modifications are discussed in detail. In addition, perovskite solar cells outside of HTMs and ETMs are mentioned as well, providing guidelines for further simplification of device processing and hence cost reduction.

  15. High Performance Perovskite Solar Cells

    PubMed Central

    Tong, Xin; Lin, Feng; Wu, Jiang

    2015-01-01

    Perovskite solar cells fabricated from organometal halide light harvesters have captured significant attention due to their tremendously low device costs as well as unprecedented rapid progress on power conversion efficiency (PCE). A certified PCE of 20.1% was achieved in late 2014 following the first study of long‐term stable all‐solid‐state perovskite solar cell with a PCE of 9.7% in 2012, showing their promising potential towards future cost‐effective and high performance solar cells. Here, notable achievements of primary device configuration involving perovskite layer, hole‐transporting materials (HTMs) and electron‐transporting materials (ETMs) are reviewed. Numerous strategies for enhancing photovoltaic parameters of perovskite solar cells, including morphology and crystallization control of perovskite layer, HTMs design and ETMs modifications are discussed in detail. In addition, perovskite solar cells outside of HTMs and ETMs are mentioned as well, providing guidelines for further simplification of device processing and hence cost reduction. PMID:27774402

  16. High-resolution prediction of leaf onset date in Japan in the 21st century under the IPCC A1B scenario.

    PubMed

    Hadano, Mayumi; Nasahara, Kenlo Nishida; Motohka, Takeshi; Noda, Hibiki Muraoka; Murakami, Kazutaka; Hosaka, Masahiro

    2013-06-01

    Reports indicate that leaf onset (leaf flush) of deciduous trees in cool-temperate ecosystems is occurring earlier in the spring in response to global warming. In this study, we created two types of phenology models, one driven only by warmth (spring warming [SW] model) and another driven by both warmth and winter chilling (parallel chill [PC] model), to predict such phenomena in the Japanese Islands at high spatial resolution (500 m). We calibrated these models using leaf onset dates derived from satellite data (Terra/MODIS) and in situ temperature data derived from a dense network of ground stations Automated Meteorological Data Acquisition System. We ran the model using future climate predictions created by the Japanese Meteorological Agency's MRI-AGCM3.1S model. In comparison to the first decade of the 2000s, our results predict that the date of leaf onset in the 2030s will advance by an average of 12 days under the SW model and 7 days under the PC model throughout the study area. The date of onset in the 2090s will advance by 26 days under the SW model and by 15 days under the PC model. The greatest impact will occur on Hokkaido (the northernmost island) and in the central mountains.

  17. High-resolution prediction of leaf onset date in Japan in the 21st century under the IPCC A1B scenario

    PubMed Central

    Hadano, Mayumi; Nasahara, Kenlo Nishida; Motohka, Takeshi; Noda, Hibiki Muraoka; Murakami, Kazutaka; Hosaka, Masahiro

    2013-01-01

    Reports indicate that leaf onset (leaf flush) of deciduous trees in cool-temperate ecosystems is occurring earlier in the spring in response to global warming. In this study, we created two types of phenology models, one driven only by warmth (spring warming [SW] model) and another driven by both warmth and winter chilling (parallel chill [PC] model), to predict such phenomena in the Japanese Islands at high spatial resolution (500 m). We calibrated these models using leaf onset dates derived from satellite data (Terra/MODIS) and in situ temperature data derived from a dense network of ground stations Automated Meteorological Data Acquisition System. We ran the model using future climate predictions created by the Japanese Meteorological Agency's MRI-AGCM3.1S model. In comparison to the first decade of the 2000s, our results predict that the date of leaf onset in the 2030s will advance by an average of 12 days under the SW model and 7 days under the PC model throughout the study area. The date of onset in the 2090s will advance by 26 days under the SW model and by 15 days under the PC model. The greatest impact will occur on Hokkaido (the northernmost island) and in the central mountains. PMID:23789086

  18. Effect of copper, manganese, and zinc supplementation on the performance, clinical signs, and mineral status of calves following exposure to bovine viral diarrhea virus type 1b and subsequent infection.

    PubMed

    Wilson, B K; Vazquez-Anon, M; Step, D L; Moyer, K D; Haviland, C L; Maxwell, C L; O'Neill, C F; Gifford, C A; Krehbiel, C R; Richards, C J

    2016-03-01

    Research has indicated that trace mineral (TM) supplementation may alter immune function and reduce morbidity associated with bovine respiratory disease. The objective of this experiment was to determine the influence of dietary Cu, Mn, and Zn supplementation on the performance, clinical signs, and TM balance of calves following a bovine viral diarrhea virus (BVDV) and (MH) combination respiratory pathogen challenge. Steers ( = 16; 225 ± 20 kg BW) from a single ranch were processed, weaned, and randomly pairwise assigned to either the TM-supplemented (MIN) or the control (CON) experimental treatments. The MIN calves received an additional 150 mg of Cu, 130 mg of Mn, and 320 mg of Zn daily and the CON calves received the basal diet with no additional Cu, Mn, or Zn supplementation. The basal diet contained sufficient Mn and Zn but inadequate Cu based on published nutrient requirements. After 46 d on the experimental treatments, all calves were naturally exposed to a heifer persistently infected with BVDV type 1b for 4 d and then subsequently intratracheally challenged with MH. Data were analyzed using the GLIMMIX procedure of SAS with sampling time serving as a repeated measure and calf serving as the experimental unit. The respiratory challenge was validated via increased BVDV type 1b antibody concentrations, MH whole cell and leukotoxin antibody concentrations, rectal temperatures (TEMP), and subjective clinical severity scores (CS). Calf performance ( ≥ 0.48) was not affected by TM supplementation. Mineral supplementation also did not impact the CS or TEMP of calves ( ≥ 0.53). There was a treatment × time ( < 0.001) interaction observed for liver Cu concentrations. The concentrations of Cu, Mn, Zn, and Fe within the liver; Cu, Mn, and Zn within the muscle; and Cu, Zn, and Fe within the serum were all impacted by time ( ≤ 0.03). Calves receiving the MIN treatment had greater ( < 0.01) liver Cu and Mn concentrations compared with CON calves. In contrast

  19. B-1B excels in conventional role

    SciTech Connect

    Scott, W.B.

    1992-07-01

    A report is presented of an observational flight performed in a USAF B-1B to better understand the operational aspects of the aircraft's new conventional bombing mission as an integral element of a multiaircraft tactical strike package. The basic flight plan consisted of a standard takeoff and climb, cruising to the training area at 22,000 ft, descending for a 400 ft low-level run, making two simulated bomb drops, and climbing back to 25,000 ft for the return to base. Attention is given the new/enhanced avionics, the ALQ-161 defensive electronic warfare system and ripple-release Mk. 82 bombing procedures.

  20. Quinazoline derivatives as selective CYP1B1 inhibitors.

    PubMed

    Mohd Siddique, Mohd Usman; McCann, Glen J P; Sonawane, Vinay R; Horley, Neill; Gatchie, Linda; Joshi, Prashant; Bharate, Sandip B; Jayaprakash, Venkatesan; Sinha, Barij N; Chaudhuri, Bhabatosh

    2017-04-21

    CYP1B1 is implicated to have a role in the development of breast, ovarian, renal, skin and lung carcinomas. It has been suggested that identification of potent and specific CYP1B1 inhibitors can lead to a novel treatment of cancer. Flavonoids have a compact rigid skeleton which fit precisely within the binding cavity of CYP1B1. Systematic isosteric replacement of flavonoid 'O' atom with 'N' atom led to the prediction that a 'quinazoline' scaffold could be the basis for designing potential CYP1B1 inhibitors. A total of 20 quinazoline analogs were synthesized and screened for CYP1B1 and CYP1A1 inhibition in Sacchrosomes™. IC50 determinations of six compounds with capability of inhibiting CYP1B1 identified quinazolines 5c and 5h as the best candidates for CYP1B1 inhibition, with IC50 values in the nM range. Further selectivity studies with homologous CYPs, belonging to the CYP1, CYP2 and CYP3 family of enzymes, showed that the compounds are likely to be free from critical drug-drug interaction liability. Molecular modelling studies were performed to rationalize the observed enzymatic inhibitions. Further biological studies in live yeast and human cells, harboring CYP1A1 and CYP1B1 enzymes, have illustrated the most potent compounds' cellular permeability and capability of potently inhibiting CYP1B1 enzyme expressed within live cells.

  1. Impact of IL1B gene polymorphisms and interleukin 1B levels on susceptibility to spontaneous preterm birth.

    PubMed

    Langmia, Immaculate M; Apalasamy, Yamunah D; Omar, Siti Z; Mohamed, Zahurin

    2016-11-01

    Genetic factors influence susceptibility to preterm birth (PTB) and the immune pathway of PTB that involves the production of cytokines such as interleukins has been implicated in PTB disease. The aim of this study is to investigate the association of interleukin 1β (IL1B) gene polymorphisms and IL1B levels with spontaneous PTB. Peripheral maternal blood from 495 women was used for extraction of DNA and genotyping was carried out using the Sequenom MassARRAY platform. Maternal plasma was used to measure IL1B levels. There was no significant association between the allelic and genotype distribution of IL1B single nucleotide polymorphism (SNP) (rs1143634, rs1143627, rs16944) and the risk of PTB among Malaysian Malay women (rs1143634, P=0.722; rs1143627, P=0.543; rs16944, P=0.615). However, IL1B levels were significantly different between women who delivered preterm compared with those who delivered at term (P=0.030); high mean levels were observed among Malay women who delivered at preterm (mean=32.52) compared with term (mean=21.68). IL1B SNPs were not associated with IL1B plasma levels. This study indicates a significant association between IL1B levels and reduced risk of PTB among the Malaysian Malay women. This study shows the impact of IL1B levels on susceptibility to PTB disease; however, the high levels of IL1B observed among women in the preterm group are not associated with IL1B SNPs investigated in this study; IL1B high levels may be because of other factors not explored in this study and therefore warrant further investigation.

  2. High Performance Solution Processable TFTs

    NASA Astrophysics Data System (ADS)

    Gundlach, David

    2008-03-01

    Organic-based electronic devices offer the potential to significantly impact the functionality and pervasiveness of large-area electronics. We report on soluble acene-based organic thin film transistors (OTFTs) where the microstructure of as-cast films can be precisely controlled via interfacial chemistry. Chemically tailoring the source/drain contact interface is a novel route to self-patterning of soluble small molecule organic semiconductors and enables the growth of highly ordered regions along opposing contact edges which extend into the transistor channel. The unique film forming properties of soluble fluorinated anthradithiophenes allows us to fabricate high performance OTFTs, OTFT circuits, and to deterministically study the influence of the film microstructure on the electrical characteristics of devices. Most recently we have grown single crystals of soluble fluorinated anthradithiophenes by vapor transport method allowing us to probe deeper into their intrinsic properties and determine the potential and limitations of this promising family of oligomers for use in organic-based electronic devices. Co-Authors: O. D. Jurchescu^1,4, B. H. Hamadani^1, S. K. Park^4, D. A. Mourey^4, S. Subramanian^5, A. J. Moad^2, R. J. Kline^3, L. C. Teague^2, J. G. Kushmerick^2, L. J. Richter^2, T. N. Jackson^4, and J. E. Anthony^5 ^1Semiconductor Electronics Division, ^2Surface and Microanalysis Science Division, ^3Polymers Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 ^4Department of Electrical Engineering, The Pennsylvania State University, University Park, PA 16802 ^5Department of Chemistry, University of Kentucky, Lexington, KY 40506-0055

  3. High performance Cu adhesion coating

    SciTech Connect

    Lee, K.W.; Viehbeck, A.; Chen, W.R.; Ree, M.

    1996-12-31

    Poly(arylene ether benzimidazole) (PAEBI) is a high performance thermoplastic polymer with imidazole functional groups forming the polymer backbone structure. It is proposed that upon coating PAEBI onto a copper surface the imidazole groups of PAEBI form a bond with or chelate to the copper surface resulting in strong adhesion between the copper and polymer. Adhesion of PAEBI to other polymers such as poly(biphenyl dianhydride-p-phenylene diamine) (BPDA-PDA) polyimide is also quite good and stable. The resulting locus of failure as studied by XPS and IR indicates that PAEBI gives strong cohesive adhesion to copper. Due to its good adhesion and mechanical properties, PAEBI can be used in fabricating thin film semiconductor packages such as multichip module dielectric (MCM-D) structures. In these applications, a thin PAEBI coating is applied directly to a wiring layer for enhancing adhesion to both the copper wiring and the polymer dielectric surface. In addition, a thin layer of PAEBI can also function as a protection layer for the copper wiring, eliminating the need for Cr or Ni barrier metallurgies and thus significantly reducing the number of process steps.

  4. ALMA high performance nutating subreflector

    NASA Astrophysics Data System (ADS)

    Gasho, Victor L.; Radford, Simon J. E.; Kingsley, Jeffrey S.

    2003-02-01

    For the international ALMA project"s prototype antennas, we have developed a high performance, reactionless nutating subreflector (chopping secondary mirror). This single axis mechanism can switch the antenna"s optical axis by +/-1.5" within 10 ms or +/-5" within 20 ms and maintains pointing stability within the antenna"s 0.6" error budget. The light weight 75 cm diameter subreflector is made of carbon fiber composite to achieve a low moment of inertia, <0.25 kg m2. Its reflecting surface was formed in a compression mold. Carbon fiber is also used together with Invar in the supporting structure for thermal stability. Both the subreflector and the moving coil motors are mounted on flex pivots and the motor magnets counter rotate to absorb the nutation reaction force. Auxiliary motors provide active damping of external disturbances, such as wind gusts. Non contacting optical sensors measure the positions of the subreflector and the motor rocker. The principle mechanical resonance around 20 Hz is compensated with a digital PID servo loop that provides a closed loop bandwidth near 100 Hz. Shaped transitions are used to avoid overstressing mechanical links.

  5. The new powder diffractometer D1B of the Institut Laue Langevin

    NASA Astrophysics Data System (ADS)

    Puente Orench, I.; Clergeau, J. F.; Martínez, S.; Olmos, M.; Fabelo, O.; Campo, J.

    2014-11-01

    D1B is a medium resolution high flux powder diffractometer located at the Institut Laue Langevin, ILL. D1B a suitable instrument for studying a large variety of polycrystalline materials. D1B runs since 1998 as a CRG (collaborating research group) instrument, being exploited by the CNRS (Centre National de la Recherche Scientifique, France) and CSIC (Consejo Superior de Investigaciones Cientificas, Spain). In 2008 the Spanish CRG started an updating program which included a new detector and a radial oscillating collimator (ROC). The detector, which has a sensitive height of 100mm, covers an angular range of 128°. Its 1280 gold wires provide a neutron detection point every 0.1°. The ROC is made of 198 gadolinium- based absorbing collimation blades, regular placed every 0.67°. Here the present characteristics of D1B are reviewed and the different experimental performances will be presented.

  6. High performance silicon optical modulators

    NASA Astrophysics Data System (ADS)

    Reed, G. T.; Thomson, D. J.; Gardes, F. Y.; Hu, Y.; Owens, N.; Debnath, K.; O'Faolain, L.; Krauss, T. F.; Lever, L.; Ikonic, Z.; Kelsall, R. W.; Myronov, M.; Leadley, D. R.; Marko, I. P.; Sweeney, S. J.; Cox, D. C.; Brimont, A.; Sanchis, P.; Duan, G.-H.; Le Liepvre, A.; Jany, C.; Lamponi, M.; Make, D.; Lelarge, F.; Fedeli, J. M.; Messaoudene, S.; Keyvaninia, S.; Roelkens, G.; Van Thourhout, D.; Liu, S.; Yang, X.; Petropoulos, P.

    2012-11-01

    In this work we present results from high performance silicon optical modulators produced within the two largest silicon photonics projects in Europe; UK Silicon Photonics (UKSP) and HELIOS. Two conventional MZI based optical modulators featuring novel self-aligned fabrication processes are presented. The first is based in 400nm overlayer SOI and demonstrates 40Gbit/s modulation with the same extinction ratio for both TE and TM polarisations, which relaxes coupling requirements to the device. The second design is based in 220nm SOI and demonstrates 40Gbits/s modulation with a 10dB extinction ratio as well modulation at 50Gbit/s for the first time. A ring resonator based optical modulator, featuring FIB error correction is presented. 40Gbit/s, 32fJ/bit operation is also shown from this device which has a 6um radius. Further to this slow light enhancement of the modulation effect is demonstrated through the use of both convention photonic crystal structures and corrugated waveguides. Fabricated conventional photonic crystal modulators have shown an enhancement factor of 8 over the fast light case. The corrugated waveguide device shows modulation efficiency down to 0.45V.cm compared to 2.2V.cm in the fast light case. 40Gbit/s modulation is demonstrated with a 3dB modulation depth from this device. Novel photonic crystal based cavity modulators are also demonstrated which offer the potential for low fibre to fibre loss. In this case preliminary modulation results at 1Gbit/s are demonstrated. Ge/SiGe Stark effect devices operating at 1300nm are presented. Finally an integrated transmitter featuring a III-V source and MZI modulator operating at 10Gbit/s is presented.

  7. CREPT/RPRD1B, a Recently Identified Novel Protein Highly Expressed in Tumors, Enhances the β-Catenin·TCF4 Transcriptional Activity in Response to Wnt Signaling*

    PubMed Central

    Zhang, Yanquan; Liu, Chunxiao; Duan, Xiaolin; Ren, Fangli; Li, Shan; Jin, Zhe; Wang, Yinyin; Feng, Yarui; Liu, Zewen; Chang, Zhijie

    2014-01-01

    CREPT (cell cycle-related and expression elevated protein in tumor)/RPRD1B (regulation of nuclear pre-mRNA domain-containing protein 1B), highly expressed during tumorigenesis, was shown to enhance transcription of CCND1 and to promote cell proliferation by interacting with RNA polymerase II. However, which signaling pathway is involved in CREPT-mediated activation of gene transcription remains unclear. In this study, we reveal that CREPT participates in transcription of the Wnt/β-catenin signaling activated genes through the β-catenin and the TCF4 complex. Our results demonstrate that CREPT interacts with both β-catenin and TCF4, and enhances the association of β-catenin with TCF4, in response to Wnt stimulation. Furthermore, CREPT was shown to occupy at TCF4 binding sites (TBS) of the promoters of Wnt-targeted genes under Wnt stimulation. Interestingly, depletion of CREPT resulted in decreased occupancy of β-catenin on TBS, and over-expression of CREPT enhances the activity of the β-catenin·TCF4 complex to initiate transcription of Wnt target genes, which results in up-regulated cell proliferation and invasion. Our study suggests that CREPT acts as an activator to promote transcriptional activity of the β-catenin·TCF4 complex in response to Wnt signaling. PMID:24982424

  8. 18 CFR 1b.2 - Scope.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Scope. 1b.2 Section 1b.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.2 Scope. This part applies to investigations...

  9. 18 CFR 1b.2 - Scope.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Scope. 1b.2 Section 1b.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.2 Scope. This part applies to investigations...

  10. 18 CFR 1b.12 - Transcripts.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Transcripts. 1b.12 Section 1b.12 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.12 Transcripts. Transcripts, if any, of...

  11. 18 CFR 1b.6 - Preliminary investigations.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Preliminary investigations. 1b.6 Section 1b.6 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.6 Preliminary investigations. The...

  12. 18 CFR 1b.6 - Preliminary investigations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Preliminary investigations. 1b.6 Section 1b.6 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.6 Preliminary investigations. The...

  13. 18 CFR 1b.12 - Transcripts.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Transcripts. 1b.12 Section 1b.12 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.12 Transcripts. Transcripts, if any, of...

  14. 18 CFR 1b.21 - Enforcement hotline.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Enforcement hotline. 1b.21 Section 1b.21 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.21 Enforcement hotline. (a) The...

  15. 18 CFR 1b.2 - Scope.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Scope. 1b.2 Section 1b.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.2 Scope. This part applies to investigations...

  16. 18 CFR 1b.2 - Scope.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Scope. 1b.2 Section 1b.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.2 Scope. This part applies to investigations...

  17. 18 CFR 1b.14 - Subpoenas.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Subpoenas. 1b.14 Section 1b.14 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.14 Subpoenas. (a) Service of a subpoena...

  18. 18 CFR 1b.21 - Enforcement hotline.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Enforcement hotline. 1b.21 Section 1b.21 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.21 Enforcement hotline. (a) The...

  19. 18 CFR 1b.19 - Submissions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Submissions. 1b.19 Section 1b.19 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.19 Submissions. In the event the...

  20. 18 CFR 1b.6 - Preliminary investigations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Preliminary investigations. 1b.6 Section 1b.6 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.6 Preliminary investigations. The...

  1. 18 CFR 1b.19 - Submissions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Submissions. 1b.19 Section 1b.19 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.19 Submissions. In the event the...

  2. 18 CFR 1b.14 - Subpoenas.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Subpoenas. 1b.14 Section 1b.14 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.14 Subpoenas. (a) Service of a subpoena...

  3. 18 CFR 1b.5 - Formal investigations.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Formal investigations. 1b.5 Section 1b.5 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.5 Formal investigations. The...

  4. 18 CFR 1b.19 - Submissions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Submissions. 1b.19 Section 1b.19 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.19 Submissions. In the event the...

  5. 18 CFR 1b.19 - Submissions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Submissions. 1b.19 Section 1b.19 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.19 Submissions. In the event the...

  6. 18 CFR 1b.14 - Subpoenas.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Subpoenas. 1b.14 Section 1b.14 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.14 Subpoenas. (a) Service of a subpoena...

  7. 18 CFR 1b.12 - Transcripts.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Transcripts. 1b.12 Section 1b.12 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.12 Transcripts. Transcripts, if any, of...

  8. 18 CFR 1b.1 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Definitions. 1b.1 Section 1b.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.1 Definitions. For purposes of this part— (a...

  9. 18 CFR 1b.12 - Transcripts.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Transcripts. 1b.12 Section 1b.12 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.12 Transcripts. Transcripts, if any, of...

  10. 18 CFR 1b.14 - Subpoenas.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Subpoenas. 1b.14 Section 1b.14 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.14 Subpoenas. (a) Service of a subpoena...

  11. 18 CFR 1b.21 - Enforcement hotline.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Enforcement hotline. 1b.21 Section 1b.21 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.21 Enforcement hotline. (a) The...

  12. 18 CFR 1b.5 - Formal investigations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Formal investigations. 1b.5 Section 1b.5 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.5 Formal investigations. The...

  13. 18 CFR 1b.6 - Preliminary investigations.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Preliminary investigations. 1b.6 Section 1b.6 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.6 Preliminary investigations. The...

  14. 18 CFR 1b.21 - Enforcement hotline.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Enforcement hotline. 1b.21 Section 1b.21 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.21 Enforcement hotline. (a) The...

  15. 18 CFR 1b.1 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Definitions. 1b.1 Section 1b.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.1 Definitions. For purposes of this part— (a...

  16. 7 CFR 1b.2 - Policy.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 1 2011-01-01 2011-01-01 false Policy. 1b.2 Section 1b.2 Agriculture Office of the Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.2 Policy. (a) All policies and programs of... Environmental Quality, will develop the necessary processes to be used by the Office of the Secretary in...

  17. 7 CFR 1b.2 - Policy.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Policy. 1b.2 Section 1b.2 Agriculture Office of the Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.2 Policy. (a) All policies and programs of... Environmental Quality, will develop the necessary processes to be used by the Office of the Secretary in...

  18. 7 CFR 1b.2 - Policy.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 1 2014-01-01 2014-01-01 false Policy. 1b.2 Section 1b.2 Agriculture Office of the Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.2 Policy. (a) All policies and programs of... Environmental Quality, will develop the necessary processes to be used by the Office of the Secretary in...

  19. 7 CFR 1b.2 - Policy.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Policy. 1b.2 Section 1b.2 Agriculture Office of the Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.2 Policy. (a) All policies and programs of... Environmental Quality, will develop the necessary processes to be used by the Office of the Secretary in...

  20. 7 CFR 1b.2 - Policy.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 1 2013-01-01 2013-01-01 false Policy. 1b.2 Section 1b.2 Agriculture Office of the Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.2 Policy. (a) All policies and programs of... Environmental Quality, will develop the necessary processes to be used by the Office of the Secretary in...

  1. 18 CFR 1b.21 - Enforcement hotline.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Enforcement hotline. 1b.21 Section 1b.21 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.21 Enforcement hotline. (a) The...

  2. 18 CFR 1b.2 - Scope.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Scope. 1b.2 Section 1b.2 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.2 Scope. This part applies to investigations...

  3. 18 CFR 1b.19 - Submissions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Submissions. 1b.19 Section 1b.19 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.19 Submissions. In the event the...

  4. 18 CFR 1b.6 - Preliminary investigations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Preliminary investigations. 1b.6 Section 1b.6 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.6 Preliminary investigations. The...

  5. 18 CFR 1b.14 - Subpoenas.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Subpoenas. 1b.14 Section 1b.14 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.14 Subpoenas. (a) Service of a subpoena...

  6. 18 CFR 1b.12 - Transcripts.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Transcripts. 1b.12 Section 1b.12 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.12 Transcripts. Transcripts, if any, of...

  7. Host range of the potential biopesticide Pea Albumin 1b (PA1b) is limited to insects.

    PubMed

    Rahioui, Isabelle; Eyraud, Vanessa; Karaki, Lamis; Sasse, Florenz; Carre-Pierrat, Maïté; Qin, An; Zheng, Ming H; Toepfer, Stefan; Sivignon, Catherine; Royer, Corinne; Da Silva, Pedro; Gressent, Frédéric

    2014-10-01

    The Pea Albumin 1 subunit b (PA1b) peptide is an entomotoxin extracted from legume seeds with lethal activity towards several insect pests. Its toxic activity occurs after the perception of PA1b by a plasmalemmic proton pump (V-ATPase) in the insects. Assays revealed that PA1b showed no activity towards mammalian cells displaying high V-ATPase activity. Similarly, PA1b displayed no binding activity and no biological activity towards other non-insect organisms. We demonstrate here that binding to labelled PA1b was found in all the insect families tested, regardless of the sensitivity or insensitivity of the individual species. The coleopteran Bruchidae, which are mainly legume seed pests, were found to be fully resistant. A number of insect species were seen to be insensitive to the toxin although they exhibited binding activity for the labelled PA1b. The fruit fly, Drosophila melanogaster (Diptera), was generally insensitive when maintained on an agar diet, but the fly appeared to be sensitive to PA1b in bioassays using a different diet. In conclusion, the PA1b toxin provides legumes with a major source of resistance to insects, and insects feeding on legume seeds need to overcome this plant resistance by disrupting the PA1b - V-ATPase interaction.

  8. Aldo-Keto Reductases 1B in Adrenal Cortex Physiology

    PubMed Central

    Pastel, Emilie; Pointud, Jean-Christophe; Martinez, Antoine; Lefrançois-Martinez, A. Marie

    2016-01-01

    Aldose reductase (AKR1B) proteins are monomeric enzymes, belonging to the aldo-keto reductase (AKR) superfamily. They perform oxidoreduction of carbonyl groups from a wide variety of substrates, such as aliphatic and aromatic aldehydes or ketones. Due to the involvement of human aldose reductases in pathologies, such as diabetic complications and cancer, AKR1B subgroup enzymatic properties have been extensively characterized. However, the issue of AKR1B function in non-pathologic conditions remains poorly resolved. Adrenal activities generated large amount of harmful aldehydes from lipid peroxidation and steroidogenesis, including 4-hydroxynonenal (4-HNE) and isocaproaldehyde (4-methylpentanal), which can both be reduced by AKR1B proteins. More recently, some AKR1B isoforms have been shown to be endowed with prostaglandin F synthase (PGFS) activity, suggesting that, in addition to possible scavenger function, they could instigate paracrine signals. Interestingly, the adrenal gland is one of the major sites for human and murine AKR1B expression, suggesting that their detoxifying/signaling activity could be specifically required for the correct handling of adrenal function. Moreover, chronic effects of ACTH result in a coordinated regulation of genes encoding the steroidogenic enzymes and some AKR1B isoforms. This review presents the molecular mechanisms accounting for the adrenal-specific expression of some AKR1B genes. Using data from recent mouse genetic models, we will try to connect their enzymatic properties and regulation with adrenal functions. PMID:27499746

  9. Interindividual and interethnic variability in drug disposition: polymorphisms in organic anion transporting polypeptide 1B1 (OATP1B1; SLCO1B1).

    PubMed

    Lee, Hannah H; Ho, Richard H

    2017-06-01

    OATP1B1 (SLCO1B1) is predominantly expressed at the basolateral membrane of hepatocytes and is critically important for the hepatic uptake and clearance of numerous drug substrates and endogenous compounds. In general, the organic anion transporting polypeptides (OATP; SLCO) represent a superfamily of uptake transporters that mediate the sodium-independent transport of a diverse range of amphipathic organic compounds including bile salts, steroid conjugates, thyroid hormones, anionic peptides, numerous drugs and other xenobiotic substances. OATP1B1 is highly polymorphic and a number of relevant and ethnically dependent polymorphisms have been identified and functionally characterized. In particular, the SLCO1B1 521T>C and 388A>G polymorphisms are commonly occurring variants in ethnically diverse populations and numerous in vitro and clinical studies have evaluated the consequences of these variants to interindividual differences in drug disposition and response. OATP1B1 is particularly important for the disposition of HMG-CoA reductase inhibitors, or statins, as it is known to efficiently transport most statins to their site of action within hepatocytes. Many studies have focused on the consequences of OATP1B1 variants to statin disposition in vitro and in vivo and would suggest that genetic variability in SLCO1B1 has important implications for statin pharmacokinetics, risk for statin-induced myopathy, and modulation of statin treatment response. This review describes what is currently known regarding SLCO1B1 genotype, OATP1B1 protein expression and interindividual and interethnic consequences to drug disposition, with particular focus on statin pharmacokinetics and implications for drug response and toxicity. © 2016 The British Pharmacological Society.

  10. High Performance Torso Cooling Garment

    NASA Technical Reports Server (NTRS)

    Conger, Bruce

    2016-01-01

    The concept proposed in this paper is to improve thermal efficiencies of the liquid cooling and ventilation garment (LCVG) in the torso area, which could facilitate removal of LCVG tubing from the arms and legs, thereby increasing suited crew member mobility. EVA space suit mobility in micro-gravity is challenging, and it becomes even more challenging in the gravity of Mars. By using shaped water tubes that greatly increase the contact area with the skin in the torso region of the body, the heat transfer efficiency can be increased. This increase in efficiency could provide the required liquid cooling via torso tubing only; no arm or leg LCVG tubing would be required. Benefits of this approach include increased crewmember mobility, reduced LCVG mass, enhanced evaporation cooling, increased comfort during Mars EVA tasks, and easing of the overly dry condition in the helmet associated with the Advanced Extravehicular Mobility Unit (EMU) ventilation loop currently under development. This report describes analysis and test activities performed to evaluate the potential improvements to the thermal performance of the LCVG. Analyses evaluated potential tube shapes for improving the thermal performance of the LCVG. The analysis results fed into the selection of flat flow strips to improve thermal contact with the skin of the suited test subject. Testing of small segments was performed to compare thermal performance of the tubing approach of the current LCVG to the flat flow strips proposed as the new concept. Results of the testing is presented along with recommendations for future development of this new concept.

  11. High Performance Torso Cooling Garment

    NASA Technical Reports Server (NTRS)

    Conger, Bruce; Makinen, Janice

    2016-01-01

    The concept proposed in this paper is to improve thermal efficiencies of the liquid cooling and ventilation garment (LCVG) in the torso area, which could facilitate removal of LCVG tubing from the arms and legs, thereby increasing suited crew member mobility. EVA space suit mobility in micro-gravity is challenging, and it becomes even more challenging in the gravity of Mars. By using shaped water tubes that greatly increase the contact area with the skin in the torso region of the body, the heat transfer efficiency can be increased. This increase in efficiency could provide the required liquid cooling via torso tubing only; no arm or leg LCVG tubing would be required. Benefits of this approach include increased crewmember mobility, enhanced evaporation cooling, increased comfort during Mars EVA tasks, and easing of the overly dry condition in the helmet associated with the Advanced Extravehicular Mobility Unit (EMU) ventilation loop currently under development. This report describes analysis and test activities performed to evaluate the potential improvements to the thermal performance of the LCVG. Analyses evaluated potential tube shapes for improving the thermal performance of the LCVG. The analysis results fed into the selection of flat flow strips to improve thermal contact with the skin of the suited test subject. Testing of small segments was performed to compare thermal performance of the tubing approach of the current LCVG to the flat flow strips proposed as the new concept. Results of the testing is presented along with recommendations for future development of this new concept.

  12. High performance parallel implicit CFD.

    SciTech Connect

    Gropp, W. D.; Kaushik, D. K.; Keyes, D. E.; Smith, B. F.; Mathematics and Computer Science; Old Dominion Univ.

    2001-03-01

    Fluid dynamical simulations based on finite discretizations on (quasi-)static grids scale well in parallel, but execute at a disappointing percentage of per-processor peak floating point operation rates without special attention to layout and access ordering of data. We document both claims from our experience with an unstructured grid CFD code that is typical of the state of the practice at NASA. These basic performance characteristics of PDE-based codes can be understood with surprisingly simple models, for which we quote earlier work, presenting primarily experimental results. The performance models and experimental results motivate algorithmic and software practices that lead to improvements in both parallel scalability and per node performance. This snapshot of ongoing work updates our 1999 Bell Prize-winning simulation on ASCI computers.

  13. High-performance composite chocolate

    NASA Astrophysics Data System (ADS)

    Dean, Julian; Thomson, Katrin; Hollands, Lisa; Bates, Joanna; Carter, Melvyn; Freeman, Colin; Kapranos, Plato; Goodall, Russell

    2013-07-01

    The performance of any engineering component depends on and is limited by the properties of the material from which it is fabricated. It is crucial for engineering students to understand these material properties, interpret them and select the right material for the right application. In this paper we present a new method to engage students with the material selection process. In a competition-based practical, first-year undergraduate students design, cost and cast composite chocolate samples to maximize a particular performance criterion. The same activity could be adapted for any level of education to introduce the subject of materials properties and their effects on the material chosen for specific applications.

  14. A high performance thermoacoustic engine

    NASA Astrophysics Data System (ADS)

    Tijani, M. E. H.; Spoelstra, S.

    2011-11-01

    In thermoacoustic systems heat is converted into acoustic energy and vice versa. These systems use inert gases as working medium and have no moving parts which makes the thermoacoustic technology a serious alternative to produce mechanical or electrical power, cooling power, and heating in a sustainable and environmentally friendly way. A thermoacoustic Stirling heat engine is designed and built which achieves a record performance of 49% of the Carnot efficiency. The design and performance of the engine is presented. The engine has no moving parts and is made up of few simple components.

  15. High performance, high density hydrocarbon fuels

    NASA Technical Reports Server (NTRS)

    Frankenfeld, J. W.; Hastings, T. W.; Lieberman, M.; Taylor, W. F.

    1978-01-01

    The fuels were selected from 77 original candidates on the basis of estimated merit index and cost effectiveness. The ten candidates consisted of 3 pure compounds, 4 chemical plant streams and 3 refinery streams. Critical physical and chemical properties of the candidate fuels were measured including heat of combustion, density, and viscosity as a function of temperature, freezing points, vapor pressure, boiling point, thermal stability. The best all around candidate was found to be a chemical plant olefin stream rich in dicyclopentadiene. This material has a high merit index and is available at low cost. Possible problem areas were identified as low temperature flow properties and thermal stability. An economic analysis was carried out to determine the production costs of top candidates. The chemical plant and refinery streams were all less than 44 cent/kg while the pure compounds were greater than 44 cent/kg. A literature survey was conducted on the state of the art of advanced hydrocarbon fuel technology as applied to high energy propellents. Several areas for additional research were identified.

  16. Toward High-Performance Organizations.

    ERIC Educational Resources Information Center

    Lawler, Edward E., III

    2002-01-01

    Reviews management changes that companies have made over time in adopting or adapting four approaches to organizational performance: employee involvement, total quality management, re-engineering, and knowledge management. Considers future possibilities and defines a new view of what constitutes effective organizational design in management.…

  17. High-Performance Composite Chocolate

    ERIC Educational Resources Information Center

    Dean, Julian; Thomson, Katrin; Hollands, Lisa; Bates, Joanna; Carter, Melvyn; Freeman, Colin; Kapranos, Plato; Goodall, Russell

    2013-01-01

    The performance of any engineering component depends on and is limited by the properties of the material from which it is fabricated. It is crucial for engineering students to understand these material properties, interpret them and select the right material for the right application. In this paper we present a new method to engage students with…

  18. High-Performance Composite Chocolate

    ERIC Educational Resources Information Center

    Dean, Julian; Thomson, Katrin; Hollands, Lisa; Bates, Joanna; Carter, Melvyn; Freeman, Colin; Kapranos, Plato; Goodall, Russell

    2013-01-01

    The performance of any engineering component depends on and is limited by the properties of the material from which it is fabricated. It is crucial for engineering students to understand these material properties, interpret them and select the right material for the right application. In this paper we present a new method to engage students with…

  19. Toward High-Performance Organizations.

    ERIC Educational Resources Information Center

    Lawler, Edward E., III

    2002-01-01

    Reviews management changes that companies have made over time in adopting or adapting four approaches to organizational performance: employee involvement, total quality management, re-engineering, and knowledge management. Considers future possibilities and defines a new view of what constitutes effective organizational design in management.…

  20. High-performance electroabsorption modulator

    NASA Astrophysics Data System (ADS)

    Wei, Zhang; Jiaoqing, Pan; Hongliang, Zhu; Huan, Wang; Wei, Wang

    2009-09-01

    A 100-μm-long electroabsorption modulator monolithically integrated with passive waveguides at the input and output ports is fabricated through ion implantation induced quantum well intermixing, using only a two-step low-pressure metal-organic vapor phase epitaxial process. An InGaAsP/InGaAsP intra-step quantum well is introduced to the active region to improve the modulation properties. In the experiment high modulation speed and high extinction ratio are obtained simultaneously, the electrical-to-optical frequency response (E/O response) without any load termination reaches to 22 GHz, and extinction ration is as high as 16 dB.

  1. Nanocrystalline high performance permanent magnets

    NASA Astrophysics Data System (ADS)

    Gutfleisch, O.; Bollero, A.; Handstein, A.; Hinz, D.; Kirchner, A.; Yan, A.; Müller, K.-H.; Schultz, L.

    2002-04-01

    Recent developments in nanocrystalline rare earth-transition metal magnets are reviewed and emphasis is placed on research work at IFW Dresden. Principal synthesis methods include high energy ball milling, melt spinning and hydrogen assisted methods such as reactive milling and hydrogenation-disproportionation-desorption-recombination. These techniques are applied to NdFeB-, PrFeB- and SmCo-type systems with the aim to produce high remanence magnets with high coercivity. Concepts of maximizing the energy density in nanostructured magnets by either inducing a texture via anisotropic HDDR or hot deformation or enhancing the remanence via magnetic exchange coupling are evaluated.

  2. Hepatitis C virus NS5A replication complex inhibitors. Part 6: Discovery of a novel and highly potent biarylimidazole chemotype with inhibitory activity toward genotypes 1a and 1b replicons.

    PubMed

    Belema, Makonen; Nguyen, Van N; Romine, Jeffrey L; St Laurent, Denis R; Lopez, Omar D; Goodrich, Jason T; Nower, Peter T; O'Boyle, Donald R; Lemm, Julie A; Fridell, Robert A; Gao, Min; Fang, Hua; Krause, Rudolph G; Wang, Ying-Kai; Oliver, A Jayne; Good, Andrew C; Knipe, Jay O; Meanwell, Nicholas A; Snyder, Lawrence B

    2014-03-13

    A medicinal chemistry campaign that was conducted to address a potential genotoxic liability associated with an aniline-derived scaffold in a series of HCV NS5A inhibitors with dual GT-1a/-1b inhibitory activity is described. Anilides 3b and 3c were used as vehicles to explore structural modifications that retained antiviral potency while removing the potential for metabolism-based unmasking of the embedded aniline. This effort resulted in the discovery of a highly potent biarylimidazole chemotype that established a potency benchmark in replicon assays, particularly toward HCV GT-1a, a strain with significant clinical importance. Securing potent GT-1a activity in a chemotype class lacking overt structural liabilities was a critical milestone in the effort to realize the full clinical potential of targeting the HCV NS5A protein.

  3. Indoor Air Quality in High Performance Schools

    EPA Pesticide Factsheets

    High performance schools are facilities that improve the learning environment while saving energy, resources, and money. The key is understanding the lifetime value of high performance schools and effectively managing priorities, time, and budget.

  4. CYP1B1 and hormone-induced cancer.

    PubMed

    Gajjar, Ketan; Martin-Hirsch, Pierre L; Martin, Francis L

    2012-11-01

    Cancers in hormone-responsive tissues (e.g., breast, ovary, endometrium, prostate) occur at high incidence rates worldwide. However, their genetic basis remains poorly understood. Studies to date suggest that endogenous/exogenous oestrogen and environmental carcinogens may play a role in development and/or progression of hormone-induced cancers via oxidative oestrogen metabolism. Cytochrome P450 1B1 is a key enzyme in its oestrogen metabolism pathway, giving rise to hydroxylation and conjugation. Although CYP1B1 is expressed in many cancers, particularly high levels of expression are observed in oestrogen-mediated disease. CYP1B1 is more readily found in tumour tissue compared to normal. Given the role of CYP1B1 in pro-carcinogen and oestrogen metabolism, polymorphisms in CYP1B1 could result in modifications in its enzyme activity and subsequently lead to hormone-mediated carcinogenesis. CYP1B1 may also be involved in progression of the disease by altering the tissue response to hormones and clinical response to chemotherapy. The exact mechanism behind these events is complex and unclear. Only a few functional single nucleotide polymorphisms of CYP1B1 are known to result in amino acid substitutions and have been extensively investigated. Studies examining the contribution of different CYP1B1 alleles to hormone-mediated cancer risks are inconsistent. The main focus of this review is to appraise the available studies linking the pathogenesis of the hormone-induced cancers to various CYP1B1 polymorphisms. Additionally, we explore the role of a neuronal protein, γ-synuclein, in CYP1B1-mediated pathogenesis.

  5. Sustaining High Performance in Bad Times.

    ERIC Educational Resources Information Center

    Bassi, Laurie J.; Van Buren, Mark A.

    1997-01-01

    Summarizes the results of the American Society for Training and Development Human Resource and Performance Management Survey of 1996 that examined the performance outcomes of downsizing and high performance work systems, explored the relationship between high performance work systems and downsizing, and asked whether some downsizing practices were…

  6. High-Performance Miniature Hygrometer

    NASA Technical Reports Server (NTRS)

    Van Zandt, Thomas R.; Kaiser, William J.; Kenny, Thomas W.; Crisp, David

    1994-01-01

    Relatively inexpensive hygrometer that occupies volume less than 4 in.(3) measures dewpoints as much as 100 degrees C below ambient temperatures, with accuracy of 0.1 degrees C. Field tests indicate accuracy and repeatability identical to those of state-of-the-art larger dewpoint hygrometers. Operates up to 100 times as fast as older hygrometers, and offers simplicity and small size needed to meet cost and performance requirements of many applications.

  7. Carpet Aids Learning in High Performance Schools

    ERIC Educational Resources Information Center

    Hurd, Frank

    2009-01-01

    The Healthy and High Performance Schools Act of 2002 has set specific federal guidelines for school design, and developed a federal/state partnership program to assist local districts in their school planning. According to the Collaborative for High Performance Schools (CHPS), high-performance schools are, among other things, healthy, comfortable,…

  8. Carpet Aids Learning in High Performance Schools

    ERIC Educational Resources Information Center

    Hurd, Frank

    2009-01-01

    The Healthy and High Performance Schools Act of 2002 has set specific federal guidelines for school design, and developed a federal/state partnership program to assist local districts in their school planning. According to the Collaborative for High Performance Schools (CHPS), high-performance schools are, among other things, healthy, comfortable,…

  9. Skylab Saturn 1B flight manual

    NASA Technical Reports Server (NTRS)

    1972-01-01

    A Saturn 1B Flight Manual provides launch vehicle systems descriptions and predicted performance data for the Skylab missions. Vehicle SL-2 (SA-206) is the baseline for this manual; but, as a result of the great similarity, the material is representative of SL-3 and SL-4 launch vehicles, also. The Flight Manual is not a control document but is intended primarily as an aid to astronauts who are training for Skylab missions. In order to provide a comprehensive reference for that purpose, the manual also contains descriptions of the ground support interfaces, prelaunch operations, and emergency procedures. Mission variables and constraints are summarized, and mission control monitoring and data flow during launch preparation and flight are discussed.

  10. High-performance solar collector

    NASA Technical Reports Server (NTRS)

    Beekley, D. C.; Mather, G. R., Jr.

    1979-01-01

    Evacuated all-glass concentric tube collector using air or liquid transfer mediums is very efficient at high temperatures. Collector can directly drive existing heating systems that are presently driven by fossil fuel with relative ease of conversion and less expense than installation of complete solar heating systems.

  11. High-performance magnetic gears

    NASA Astrophysics Data System (ADS)

    Atallah, Kais; Calverley, Stuart D.; Howe, David

    2004-05-01

    Magnetic gearing may offer significant advantages such as reduced maintenance and improved reliability, inherent overload protection, and physical isolation between input and output shafts. Despite these advantages, it has received relatively little attention, to date, probably due to the poor torque transmission capability of proposed magnetic gears. The paper describes a magnetic gear topology, which combines a significantly higher torque transmission capability and a very high efficiency.

  12. High performance rotational vibration isolator

    NASA Astrophysics Data System (ADS)

    Sunderland, Andrew; Blair, David G.; Ju, Li; Golden, Howard; Torres, Francis; Chen, Xu; Lockwood, Ray; Wolfgram, Peter

    2013-10-01

    We present a new rotational vibration isolator with an extremely low resonant frequency of 0.055 ± 0.002 Hz. The isolator consists of two concentric spheres separated by a layer of water and joined by very soft silicone springs. The isolator reduces rotation noise at all frequencies above its resonance which is very important for airborne mineral detection. We show that more than 40 dB of isolation is achieved in a helicopter survey for rotations at frequencies between 2 Hz and 20 Hz. Issues affecting performance such as translation to rotation coupling and temperature are discussed. The isolator contains almost no metal, making it particularly suitable for electromagnetic sensors.

  13. High performance rotational vibration isolator.

    PubMed

    Sunderland, Andrew; Blair, David G; Ju, Li; Golden, Howard; Torres, Francis; Chen, Xu; Lockwood, Ray; Wolfgram, Peter

    2013-10-01

    We present a new rotational vibration isolator with an extremely low resonant frequency of 0.055 ± 0.002 Hz. The isolator consists of two concentric spheres separated by a layer of water and joined by very soft silicone springs. The isolator reduces rotation noise at all frequencies above its resonance which is very important for airborne mineral detection. We show that more than 40 dB of isolation is achieved in a helicopter survey for rotations at frequencies between 2 Hz and 20 Hz. Issues affecting performance such as translation to rotation coupling and temperature are discussed. The isolator contains almost no metal, making it particularly suitable for electromagnetic sensors.

  14. Introduction to Session 1B

    NASA Astrophysics Data System (ADS)

    Sticklen, Mariam B.

    Topics presented in the "Plant Biotechnology and Genomics" session focused on technologies that highlight the important role of plant biotechnology and genomics in the development of future energy crops. Several excellent presentations demonstrated the latest advances in energy crop development through the use of plant cell wall regulation and by engineering new energy crops such as brown midrib sweet sorghum. Approaches included the control of cellulose production by increased expression of cellulase synthase genes and the selection of high-yield varieties of shrub willows. The potential of producing hydrolytic enzymes using transgenic plants as a cost-effective means for the large-scale production of these enzymes was also explored in the session, as was the role of posttranslational modifications on the activities of heterologous expressed cellulases in hosts such as Pichia pastoris.

  15. A tough high performance composite matrix

    NASA Technical Reports Server (NTRS)

    Pater, Ruth H. (Inventor); Johnston, Norman J. (Inventor)

    1992-01-01

    This invention is a semi-interpenetrating polymer network which includes a high performance thermosetting polyimide having a nadic end group acting as a crosslinking site and a high performance linear thermoplastic polyimide. An improved high temperature matrix resin is provided which is capable of performing in the 200 to 300 C range. This resin has significantly improved toughness and microcracking resistance, excellent processability, mechanical performance and moisture and solvent resistances.

  16. High-Performance Synthetic Fibers for Composites

    DTIC Science & Technology

    1992-04-01

    series under subject contracts. 1989- 1955 4. TITLE AND SUBTITLE 5. FUNDING NUMBERS High-Performance Synthetic Fibers for Composites MDA 903-89-K-0078...MDA 972-92-C-0028 6. AUTHOR(S) Committee on High-Performance Synthetic Fibers for Composites 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) S...Maximum 200 words) This report describes the properties of the principal classes of high-performance synthetic fibers , as well as several current and

  17. Identification of Novel Inhibitors of Organic Anion Transporting Polypeptides 1B1 and 1B3 (OATP1B1 and OATP1B3) Using a Consensus Vote of Six Classification Models

    PubMed Central

    2015-01-01

    Organic anion transporting polypeptides 1B1 and 1B3 are transporters selectively expressed on the basolateral membrane of the hepatocyte. Several studies reveal that they are involved in drug–drug interactions, cancer, and hyperbilirubinemia. In this study, we developed a set of classification models for OATP1B1 and 1B3 inhibition based on more than 1700 carefully curated compounds from literature, which were validated via cross-validation and by use of an external test set. After combining several sets of descriptors and classifiers, the 6 best models were selected according to their statistical performance and were used for virtual screening of DrugBank. Consensus scoring of the screened compounds resulted in the selection and purchase of nine compounds as potential dual inhibitors and of one compound as potential selective OATP1B3 inhibitor. Biological testing of the compounds confirmed the validity of the models, yielding an accuracy of 90% for OATP1B1 and 80% for OATP1B3, respectively. Moreover, at least half of the new identified inhibitors are associated with hyperbilirubinemia or hepatotoxicity, implying a relationship between OATP inhibition and these severe side effects. PMID:26469880

  18. Protein tyrosine phosphatase 1B inhibitors isolated from Artemisia roxburghiana.

    PubMed

    Shah, Muhammad Raza; Ishtiaq; Hizbullah, Syed Muhammad; Habtemariam, Solomon; Zarrelli, Armando; Muhammad, Akhtar; Collina, Simona; Khan, Inamulllah

    2016-08-01

    Artemisia roxburghiana is used in traditional medicine for treating various diseases including diabetes. The present study was designed to evaluate the antidiabetic potential of active constituents by using protein tyrosine phosphatase 1B (PTP1B) as a validated target for management of diabetes. Various compounds were isolated as active principles from the crude methanolic extract of aerial parts of A. roxburghiana. All compounds were screened for PTP1B inhibitory activity. Molecular docking simulations were performed to investigate the mechanism behind PTP1B inhibition of the isolated compound and positive control, ursolic acid. Betulinic acid, betulin and taraxeryl acetate were the active PTP1B principles with IC50 values 3.49 ± 0.02, 4.17 ± 0.03 and 87.52 ± 0.03 µM, respectively. Molecular docking studies showed significant molecular interactions of the triterpene inhibitors with Gly220, Cys215, Gly218 and Asp48 inside the active site of PTP1B. The antidiabetic activity of A. roxburghiana could be attributed due to PTP1B inhibition by its triterpene constituents, betulin, betulinic acid and taraxeryl acetate. Computational insights of this study revealed that the C-3 and C-17 positions of the compounds needs extensive optimization for the development of new lead compounds.

  19. High performance light emitting transistors

    NASA Astrophysics Data System (ADS)

    Namdas, Ebinazar B.; Ledochowitsch, Peter; Yuen, Jonathan D.; Moses, Daniel; Heeger, Alan J.

    2008-05-01

    Solution processed light emitting field-effect transistors (LEFETs) with peak brightness exceeding 2500cd/m2 and external quantum efficiency of 0.15% are demonstrated. The devices utilized a bilayer film comprising a hole transporting polymer, poly(2,5-bis(3-tetradecylthiophen-2-yl)thieno[3,2-b] thiophene) and a light emitting polymer, Super Yellow, a polyphenylenevinylene derivative. The LEFETs were fabricated in the bottom gate architecture with top-contact Ca /Ag as source/drain electrodes. Light emission was controlled by the gate voltage which controls the hole current. These results indicate that high brightness LEFETs can be made by using the bilayer film (hole transporting layer and a light emitting polymer).

  20. Designing high-performance jobs.

    PubMed

    Simons, Robert

    2005-01-01

    Tales of great strategies derailed by poor execution are all too common. That's because some organizations are designed to fail. For a company to achieve its potential, each employee's supply of organizational resources should equal the demand, and the same balance must apply to every business unit and to the company as a whole. To carry out his or her job, each employee has to know the answers to four basic questions: What resources do I control to accomplish my tasks? What measures will be used to evaluate my performance? Who do I need to interact with and influence to achieve my goals? And how much support can I expect when I reach out to others for help? The questions correspond to what the author calls the four basic spans of a job-control, accountability, influence, and support. Each span can be adjusted so that it is narrow or wide or somewhere in between. If you get the settings right, you can design a job in which a talented individual can successfully execute on your company's strategy. If you get the settings wrong, it will be difficult for an employee to be effective. The first step is to set the span of control to reflect the resources allocated to each position and unit that plays an important role in delivering customer value. This setting, like the others, is determined by how the business creates value for customers and differentiates its products and services. Next, you can dial in different levels of entrepreneurial behavior and creative tension by widening or narrowing spans of accountability and influence. Finally, you must adjust the span of support to ensure that the job or unit will get the informal help it needs.

  1. HIGH-PERFORMANCE COATING MATERIALS

    SciTech Connect

    SUGAMA,T.

    2007-01-01

    Corrosion, erosion, oxidation, and fouling by scale deposits impose critical issues in selecting the metal components used at geothermal power plants operating at brine temperatures up to 300 C. Replacing these components is very costly and time consuming. Currently, components made of titanium alloy and stainless steel commonly are employed for dealing with these problems. However, another major consideration in using these metals is not only that they are considerably more expensive than carbon steel, but also the susceptibility of corrosion-preventing passive oxide layers that develop on their outermost surface sites to reactions with brine-induced scales, such as silicate, silica, and calcite. Such reactions lead to the formation of strong interfacial bonds between the scales and oxide layers, causing the accumulation of multiple layers of scales, and the impairment of the plant component's function and efficacy; furthermore, a substantial amount of time is entailed in removing them. This cleaning operation essential for reusing the components is one of the factors causing the increase in the plant's maintenance costs. If inexpensive carbon steel components could be coated and lined with cost-effective high-hydrothermal temperature stable, anti-corrosion, -oxidation, and -fouling materials, this would improve the power plant's economic factors by engendering a considerable reduction in capital investment, and a decrease in the costs of operations and maintenance through optimized maintenance schedules.

  2. AKR1B10 — EDRN Public Portal

    Cancer.gov

    The AKR1B10 protein is a member of the aldo/keto reductase superfamily. This superfamily has more than 40 known enzymes and proteins. AKR1B10 acts as an all-trans-retinaldehyde reductase. It can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis.

  3. 18 CFR 1b.5 - Formal investigations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Formal investigations. 1b.5 Section 1b.5 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION.... Orders of Investigation will outline the basis for the investigation, the matters to be investigated, the...

  4. 18 CFR 1b.5 - Formal investigations.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Formal investigations. 1b.5 Section 1b.5 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION.... Orders of Investigation will outline the basis for the investigation, the matters to be investigated, the...

  5. 18 CFR 1b.1 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Definitions. 1b.1 Section 1b.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF... and informally any matter within the Commission's jurisdiction concerning natural gas pipelines, oil...

  6. 18 CFR 1b.1 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Definitions. 1b.1 Section 1b.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF... and informally any matter within the Commission's jurisdiction concerning natural gas pipelines, oil...

  7. 18 CFR 1b.1 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Definitions. 1b.1 Section 1b.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF... and informally any matter within the Commission's jurisdiction concerning natural gas pipelines, oil...

  8. 18 CFR 1b.5 - Formal investigations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Formal investigations. 1b.5 Section 1b.5 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION.... Orders of Investigation will outline the basis for the investigation, the matters to be investigated, the...

  9. DSCOVR_EPIC_L1B

    Atmospheric Science Data Center

    2017-01-04

    DSCOVR_EPIC_L1B Full sun-light Earth images, georectified to the same ... 551NM 680NM 688NM 764NM 780NM DSCOVR EPIC IMAGERY L1B LAGRANGE Order Data:  Earthdata ...   Order Data Readme Files:  EPIC Data Format Control Book SCAR-B Block:  ...

  10. 36 CFR 261.1b - Penalty.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... of not more than $500 or imprisonment for not more than six months or both pursuant to title 16 U.S.C... 36 Parks, Forests, and Public Property 2 2012-07-01 2012-07-01 false Penalty. 261.1b Section 261.1b Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE PROHIBITIONS General...

  11. 36 CFR 261.1b - Penalty.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... of not more than $500 or imprisonment for not more than six months or both pursuant to title 16 U.S.C... 36 Parks, Forests, and Public Property 2 2013-07-01 2013-07-01 false Penalty. 261.1b Section 261.1b Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE PROHIBITIONS General...

  12. 36 CFR 261.1b - Penalty.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... of not more than $500 or imprisonment for not more than six months or both pursuant to title 16 U.S.C... 36 Parks, Forests, and Public Property 2 2010-07-01 2010-07-01 false Penalty. 261.1b Section 261.1b Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE PROHIBITIONS General...

  13. Statistical properties of high performance cesium standards

    NASA Technical Reports Server (NTRS)

    Percival, D. B.

    1973-01-01

    The intermediate term frequency stability of a group of new high-performance cesium beam tubes at the U.S. Naval Observatory were analyzed from two viewpoints: (1) by comparison of the high-performance standards to the MEAN(USNO) time scale and (2) by intercomparisons among the standards themselves. For sampling times up to 5 days, the frequency stability of the high-performance units shows significant improvement over older commercial cesium beam standards.

  14. Association of glycogen storage disease 1b and Crohn disease: results of a North American survey.

    PubMed

    Dieckgraefe, Brian K; Korzenik, Joshua R; Husain, Asif; Dieruf, Lisa

    2002-10-01

    Glycogen storage disease (GSD)-1b has been associated with neutropenia and abnormalities of neutrophil function. Many individuals with GSD-1b manifest chronic gastrointestinal inflammation. Our study was performed to precisely establish the type, frequency, and spectrum of gastrointestinal disease in patients with GSD-lb. We established a medical database of 36 individuals affected with GSD-1b, utilizing patients at our center, disease registries, and direct contacts with North American referral centers specializing in genetic disorders. Records were reviewed, focusing on documentation of gastrointestinal involvement. Gastrointestinal symptoms or manifestations were present in approximately 75% of the patients, including chronic abdominal pain and diarrhea, perianal fistula or abscess, and oral aphthous ulceration. Of patients identified, 28% had documented inflammatory bowel disease (IBD) and an additional 22% of patients had symptoms highly suggestive of IBD, but had not undergone comprehensive diagnostic evaluation. Gastrointestinal involvement was found to be indistinguishable from idiopathic Crohn disease (CD) by detailed review of clinical, radiologic, endoscopic and histopathologic findings. Many patients had significant improvement or resolution of gastrointestinal disease in response to G-CSF treatment. The expression of CD in patients with GSD-1b may be delayed or prevented by G-CSF treatment. a strong association exists between glycogen storage disease type 1b and inflammatory bowel disease. A high index of suspicion for Crohn disease should be applied in evaluating patients with glycogen storage disease type 1b and intestinal symptoms. These results provide further support for the hypothesis that some forms of inflammatory bowel disease may result from impaired mucosal innate immunity. Additional investigations into the intestinal disease in glycogen storage disease type 1b may be directly relevant to the etiology and treatment of idiopathic Crohn

  15. Method of making a high performance ultracapacitor

    DOEpatents

    Farahmandi, C. Joseph; Dispennette, John M.

    2000-07-26

    A high performance double layer capacitor having an electric double layer formed in the interface between activated carbon and an electrolyte is disclosed. The high performance double layer capacitor includes a pair of aluminum impregnated carbon composite electrodes having an evenly distributed and continuous path of aluminum impregnated within an activated carbon fiber preform saturated with a high performance electrolytic solution. The high performance double layer capacitor is capable of delivering at least 5 Wh/kg of useful energy at power ratings of at least 600 W/kg.

  16. High performance carbon nanocomposites for ultracapacitors

    DOEpatents

    Lu, Wen

    2012-10-02

    The present invention relates to composite electrodes for electrochemical devices, particularly to carbon nanotube composite electrodes for high performance electrochemical devices, such as ultracapacitors.

  17. Strategy Guideline: High Performance Residential Lighting

    SciTech Connect

    Holton, J.

    2012-02-01

    The Strategy Guideline: High Performance Residential Lighting has been developed to provide a tool for the understanding and application of high performance lighting in the home. The high performance lighting strategies featured in this guide are drawn from recent advances in commercial lighting for application to typical spaces found in residential buildings. This guide offers strategies to greatly reduce lighting energy use through the application of high quality fluorescent and light emitting diode (LED) technologies. It is important to note that these strategies not only save energy in the home but also serve to satisfy the homeowner's expectations for high quality lighting.

  18. High Resolution Spectroscopy of A^1B1u ← X^1A_g 8^1_04^1_0 Band of Naphthalene Referenced to AN Optical Frequency Comb

    NASA Astrophysics Data System (ADS)

    Nakashima, Kazuki; Nishiyama, Akiko; Misono, Masatoshi

    2016-06-01

    In the excited vibronic states of naphthalene, there exist various interesting interactions such as intramolecular vibrational energy redistribution (IVR), intersystem crossing (ISC), and internal conversion (IC). More than thirty yeas ago, Beck et al. showed that IVR became remarkable when the excess energy exceeded about 2000 cm-1^, ^a. In the present study, we observe Doppler-free two-photon absorption spectra of A^1B1u ← X^1A_g 8^1_04^1_0 band of naphthalene around 34281 cm-1. The excess energy is 2261 cm-1, which is just above the threshold of IVR. Thus we expect this band is suitable to analyze the dynamics in the excited vibronic states. In our experiment, the spectral resolution is about 100 kHz, and rovibronic lines are well-resolved. To decide the transition frequencies, frequency shifts, and spectral linewidths with high accuracy and precision, we employed the comb-referenced Doppler-free two-photon absorption spectroscopic system^b. We proceed to assign the rovibronic lines in ^qQ transition, and to determine molecular constants in the excited vibronic state. ^a S. M. Beck, J. B. Hopkins, D. E. Powers, and R. E. Smalley, J. Chem. Phys. 74, 43(1981). ^b A. Nishiyama, K. Nakashima, A. Matsuba, and M. Misono, J. Mol. Spectrosc. 318, 40 (2015).

  19. Association of Transcription Factor Gene LMX1B with Autism

    PubMed Central

    Yamada, Kazuo; Iwayama, Yoshimi; Toyota, Tomoko; Tsujii, Masatsugu; Iwata, Yasuhide; Suzuki, Katsuaki; Matsuzaki, Hideo; Iwata, Keiko; Sugiyama, Toshiro; Yoshikawa, Takeo; Mori, Norio

    2011-01-01

    Multiple lines of evidence suggest a serotoninergic dysfunction in autism. The role of LMX1B in the development and maintenance of serotoninergic neurons is well known. In order to examine the role, if any, of LMX1B with autism pathophysiology, a trio-based SNP association study using 252 family samples from the AGRE was performed. Using pair-wise tagging method, 24 SNPs were selected from the HapMap data, based on their location and minor allele frequency. Two SNPs (rs10732392 and rs12336217) showed moderate association with autism with p values 0.018 and 0.022 respectively in transmission disequilibrium test. The haplotype AGCGTG also showed significant association (p = 0.008). Further, LMX1B mRNA expressions were studied in the postmortem brain tissues of autism subjects and healthy controls samples. LMX1B transcripts was found to be significantly lower in the anterior cingulate gyrus region of autism patients compared with controls (p = 0.049). Our study suggests a possible role of LMX1B in the pathophysiology of autism. Based on previous reports, it is likely to be mediated through a seretoninergic mechanism. This is the first report on the association of LMX1B with autism, though it should be viewed with some caution considering the modest associations we report. PMID:21901133

  20. Probabilistic performance-based design for high performance control systems

    NASA Astrophysics Data System (ADS)

    Micheli, Laura; Cao, Liang; Gong, Yongqiang; Cancelli, Alessandro; Laflamme, Simon; Alipour, Alice

    2017-04-01

    High performance control systems (HPCS) are advanced damping systems capable of high damping performance over a wide frequency bandwidth, ideal for mitigation of multi-hazards. They include active, semi-active, and hybrid damping systems. However, HPCS are more expensive than typical passive mitigation systems, rely on power and hardware (e.g., sensors, actuators) to operate, and require maintenance. In this paper, a life cycle cost analysis (LCA) approach is proposed to estimate the economic benefit these systems over the entire life of the structure. The novelty resides in the life cycle cost analysis in the performance based design (PBD) tailored to multi-level wind hazards. This yields a probabilistic performance-based design approach for HPCS. Numerical simulations are conducted on a building located in Boston, MA. LCA are conducted for passive control systems and HPCS, and the concept of controller robustness is demonstrated. Results highlight the promise of the proposed performance-based design procedure.

  1. Common Factors of High Performance Teams

    ERIC Educational Resources Information Center

    Jackson, Bruce; Madsen, Susan R.

    2005-01-01

    Utilization of work teams is now wide spread in all types of organizations throughout the world. However, an understanding of the important factors common to high performance teams is rare. The purpose of this content analysis is to explore the literature and propose findings related to high performance teams. These include definition and types,…

  2. Turning High-Poverty Schools into High-Performing Schools

    ERIC Educational Resources Information Center

    Parrett, William H.; Budge, Kathleen

    2012-01-01

    If some schools can overcome the powerful and pervasive effects of poverty to become high performing, shouldn't any school be able to do the same? Shouldn't we be compelled to learn from those schools? Although schools alone will never systemically eliminate poverty, high-poverty, high-performing (HP/HP) schools take control of what they can to…

  3. Turning High-Poverty Schools into High-Performing Schools

    ERIC Educational Resources Information Center

    Parrett, William H.; Budge, Kathleen

    2012-01-01

    If some schools can overcome the powerful and pervasive effects of poverty to become high performing, shouldn't any school be able to do the same? Shouldn't we be compelled to learn from those schools? Although schools alone will never systemically eliminate poverty, high-poverty, high-performing (HP/HP) schools take control of what they can to…

  4. High performance computing at Sandia National Labs

    SciTech Connect

    Cahoon, R.M.; Noe, J.P.; Vandevender, W.H.

    1995-10-01

    Sandia`s High Performance Computing Environment requires a hierarchy of resources ranging from desktop, to department, to centralized, and finally to very high-end corporate resources capable of teraflop performance linked via high-capacity Asynchronous Transfer Mode (ATM) networks. The mission of the Scientific Computing Systems Department is to provide the support infrastructure for an integrated corporate scientific computing environment that will meet Sandia`s needs in high-performance and midrange computing, network storage, operational support tools, and systems management. This paper describes current efforts at SNL/NM to expand and modernize centralized computing resources in support of this mission.

  5. The origin of unequal bond lengths in the C1B2 state of SO2: Signatures of high-lying potential energy surface crossings in the low-lying vibrational structure

    DOE PAGES

    Park, G. Barratt; Jiang, Jun; Field, Robert W.

    2016-04-14

    Here the C1B2 state of SO2 has a double-minimum potential in the antisymmetric stretch coordinate, such that the minimum energy geometry has nonequivalent SO bond lengths. The asymmetry in the potential energy surface is expressed as a staggering in the energy levels of the v'3 progression. We have recently made the first observation of low-lying levels with odd quanta of v'3, which allows us--in the current work--to characterize the origins of the level staggering. Our work demonstrates the usefulness of low-lying vibrational level structure, where the character of the wavefunctions can be relatively easily understood, to extract information about dynamicallymore » important potential energy surface crossings that occur at much higher energy. The measured staggering pattern is consistent with a vibronic coupling model for the double-minimum, which involves direct coupling to the bound 2 1A1 state and indirect coupling with the repulsive 3 1A1 state. The degree of staggering in the v'3 levels increases with quanta of bending excitation, which is consistent with the approach along the C state potential energy surface to a conical intersection with the 2 1A1 surface at a bond angle of ~145°.« less

  6. The origin of unequal bond lengths in the C ˜ 1B2 state of SO2: Signatures of high-lying potential energy surface crossings in the low-lying vibrational structure

    NASA Astrophysics Data System (ADS)

    Park, G. Barratt; Jiang, Jun; Field, Robert W.

    2016-04-01

    The C ˜ 1B2 state of SO2 has a double-minimum potential in the antisymmetric stretch coordinate, such that the minimum energy geometry has nonequivalent SO bond lengths. The asymmetry in the potential energy surface is expressed as a staggering in the energy levels of the ν3' progression. We have recently made the first observation of low-lying levels with odd quanta of v3', which allows us—in the current work—to characterize the origins of the level staggering. Our work demonstrates the usefulness of low-lying vibrational level structure, where the character of the wavefunctions can be relatively easily understood, to extract information about dynamically important potential energy surface crossings that occur at much higher energy. The measured staggering pattern is consistent with a vibronic coupling model for the double-minimum, which involves direct coupling to the bound 2 1A1 state and indirect coupling with the repulsive 3 1A1 state. The degree of staggering in the ν3' levels increases with quanta of bending excitation, which is consistent with the approach along the C ˜ state potential energy surface to a conical intersection with the 2 1A1 surface at a bond angle of ˜145°.

  7. The origin of unequal bond lengths in the C̃ (1)B2 state of SO2: Signatures of high-lying potential energy surface crossings in the low-lying vibrational structure.

    PubMed

    Park, G Barratt; Jiang, Jun; Field, Robert W

    2016-04-14

    The C̃ (1)B2 state of SO2 has a double-minimum potential in the antisymmetric stretch coordinate, such that the minimum energy geometry has nonequivalent SO bond lengths. The asymmetry in the potential energy surface is expressed as a staggering in the energy levels of the ν3(') progression. We have recently made the first observation of low-lying levels with odd quanta of v3('), which allows us-in the current work-to characterize the origins of the level staggering. Our work demonstrates the usefulness of low-lying vibrational level structure, where the character of the wavefunctions can be relatively easily understood, to extract information about dynamically important potential energy surface crossings that occur at much higher energy. The measured staggering pattern is consistent with a vibronic coupling model for the double-minimum, which involves direct coupling to the bound 2 (1)A1 state and indirect coupling with the repulsive 3 (1)A1 state. The degree of staggering in the ν3(') levels increases with quanta of bending excitation, which is consistent with the approach along the C̃ state potential energy surface to a conical intersection with the 2 (1)A1 surface at a bond angle of ∼145°.

  8. FOLH1B — EDRN Public Portal

    Cancer.gov

    FOLH1B, or PSMAL, is a cytoplasmic protein. It has been found in the kidney and liver, and has not been detected in the prostate. GO annotations related to this gene include metallopeptidase activity and dipeptidase activity.

  9. DSCOVR_EPIC_L1B_2

    Atmospheric Science Data Center

    2017-10-01

    ... Tools:  Earthdata Search:   Order Data Search and Order:   ASDC Order Tool OPeNDAP Access:   OPeNDAP ... DSCOVR EPIC IMAGERY L1B LAGRANGE Order Data:  Earthdata Search:   Order Data Guide ...

  10. Specificity Determinants of CYP1B1 Estradiol Hydroxylation

    PubMed Central

    Nishida, Clinton R.; Everett, Steven

    2013-01-01

    Cytochrome P450 (P450)-catalyzed oxidation of the aromatic ring of estradiol can result in 2- or 4-hydroxylation. Which of these products is formed is biologically important, as the 4-hydroxylated metabolite is carcinogenic, whereas the 2-hydroxylated metabolite is not. Most human P450 enzymes, including CYP1A1 and CYP1A2, exhibit a high preference for estradiol 2-hydroxylation, but human CYP1B1 greatly favors 4-hydroxylation. Here we show that heterologous expression of the human, monkey, dog, rat, and mouse CYP1B1 enzymes yields active proteins that differ in their estradiol hydroxylation specificity. The monkey and dog orthologs, like the human enzyme, preferentially catalyze 4-hydroxylation, but the rat and mouse enzymes favor 2-hydroxylation. Analysis of the CYP1B1 sequences in light of these findings suggested that one residue, Val395 in human CYP1B1, could account for the differential hydroxylation specificities. In fact, mutation of this valine in human CYP1B1 to the leucine present in the rat enzyme produces a human enzyme that has the 2-hydroxylation specificity of the rat enzyme. The converse is true when the leucine in the rat enzyme is mutated to the human valine. The role of CYP1B1 in estradiol carcinogenicity thus depends on the identity of this single amino acid residue. PMID:23821647

  11. LANL High-Performance Data System (HPDS)

    NASA Technical Reports Server (NTRS)

    Collins, M. William; Cook, Danny; Jones, Lynn; Kluegel, Lynn; Ramsey, Cheryl

    1993-01-01

    The Los Alamos High-Performance Data System (HPDS) is being developed to meet the very large data storage and data handling requirements of a high-performance computing environment. The HPDS will consist of fast, large-capacity storage devices that are directly connected to a high-speed network and managed by software distributed in workstations. The HPDS model, the HPDS implementation approach, and experiences with a prototype disk array storage system are presented.

  12. Strategy Guideline. Partnering for High Performance Homes

    SciTech Connect

    Prahl, Duncan

    2013-01-01

    High performance houses require a high degree of coordination and have significant interdependencies between various systems in order to perform properly, meet customer expectations, and minimize risks for the builder. Responsibility for the key performance attributes is shared across the project team and can be well coordinated through advanced partnering strategies. For high performance homes, traditional partnerships need to be matured to the next level and be expanded to all members of the project team including trades, suppliers, manufacturers, HERS raters, designers, architects, and building officials as appropriate. This guide is intended for use by all parties associated in the design and construction of high performance homes. It serves as a starting point and features initial tools and resources for teams to collaborate to continually improve the energy efficiency and durability of new houses.

  13. ADVANCED HIGH PERFORMANCE SOLID WALL BLANKET CONCEPTS

    SciTech Connect

    WONG, CPC; MALANG, S; NISHIO, S; RAFFRAY, R; SAGARA, S

    2002-04-01

    OAK A271 ADVANCED HIGH PERFORMANCE SOLID WALL BLANKET CONCEPTS. First wall and blanket (FW/blanket) design is a crucial element in the performance and acceptance of a fusion power plant. High temperature structural and breeding materials are needed for high thermal performance. A suitable combination of structural design with the selected materials is necessary for D-T fuel sufficiency. Whenever possible, low afterheat, low chemical reactivity and low activation materials are desired to achieve passive safety and minimize the amount of high-level waste. Of course the selected fusion FW/blanket design will have to match the operational scenarios of high performance plasma. The key characteristics of eight advanced high performance FW/blanket concepts are presented in this paper. Design configurations, performance characteristics, unique advantages and issues are summarized. All reviewed designs can satisfy most of the necessary design goals. For further development, in concert with the advancement in plasma control and scrape off layer physics, additional emphasis will be needed in the areas of first wall coating material selection, design of plasma stabilization coils, consideration of reactor startup and transient events. To validate the projected performance of the advanced FW/blanket concepts the critical element is the need for 14 MeV neutron irradiation facilities for the generation of necessary engineering design data and the prediction of FW/blanket components lifetime and availability.

  14. Down-regulated expression of the protein-tyrosine phosphatase 1B (PTP1B) is associated with aggressive clinicopathologic features and poor prognosis in hepatocellular carcinoma

    SciTech Connect

    Zheng, Long-Yi; Zhou, Dong-Xun; Lu, Jin; Zhang, Wen-Jun; Zou, Da-Jin

    2012-04-13

    Highlights: Black-Right-Pointing-Pointer PTP1B protein showed decreased expression in 67.79% of the HCC patients. Black-Right-Pointing-Pointer Low PTP1B expression predicts poor prognosis of HCC. Black-Right-Pointing-Pointer Low PTP1B expression is correlated with expansion of OV6{sup +} tumor-initiating cells. Black-Right-Pointing-Pointer Down-regulation of PTP1B is associated with activation of Wnt/{beta}-Catenin signaling. -- Abstract: The protein-tyrosine phosphatase 1B (PTP1B) is a classical non-transmembrane protein tyrosine phosphatase that plays a key role in metabolic signaling and can exert both tumor suppressing and tumor promoting effects in different cancers depending on the substrate involved and the cellular context. However, the expression level and function of PTP1B in hepatocellular carcinoma (HCC) remain unclear. In this study, PTP1B expression was detected by immunohistochemistry in normal liver tissue (n = 16) and hepatocellular carcinoma (n = 169). The correlations between PTP1B expression level and clinicopathologic features and patient survival were also analyzed. One hundred and eleven of 169 HCC patients (65.7%) had negative or low PTP1B expression in tumorous tissues, whereas normal tissues always expressed strong PTP1B. Decreased PTP1B expression was significantly associated with aggressive clinicopathologic features and poor prognosis. Immunohistochemistry also showed that low PTP1B expression level was correlated with high percentage of OV6{sup +} tumor-initiating cells (T-ICs) and high frequency of nuclear {beta}-Catenin expression in HCC specimens. Our findings demonstrate for the first time that the loss of inhibitory effect of PTP1B may contribute to progression and invasion of HCC through activation of Wnt/{beta}-Catenin signaling and expansion of liver T-ICs. PTP1B may serve as a valuable prognostic biomarker and potential therapeutic target in HCC.

  15. Advanced high-performance computer system architectures

    NASA Astrophysics Data System (ADS)

    Vinogradov, V. I.

    2007-02-01

    Convergence of computer systems and communication technologies are moving to switched high-performance modular system architectures on the basis of high-speed switched interconnections. Multi-core processors become more perspective way to high-performance system, and traditional parallel bus system architectures (VME/VXI, cPCI/PXI) are moving to new higher speed serial switched interconnections. Fundamentals in system architecture development are compact modular component strategy, low-power processor, new serial high-speed interface chips on the board, and high-speed switched fabric for SAN architectures. Overview of advanced modular concepts and new international standards for development high-performance embedded and compact modular systems for real-time applications are described.

  16. Impact of Chronic Hepatitis C Virus Genotype 1b Infection on Triglyceride Concentration in Serum Lipoprotein Fractions.

    PubMed

    Nagano, Tomohisa; Seki, Nobuyoshi; Tomita, Yoichi; Sugita, Tomonori; Aida, Yuta; Itagaki, Munenori; Sutoh, Satoshi; Abe, Hiroshi; Tsubota, Akihito; Aizawa, Yoshio

    2015-08-31

    Reduced low-density lipoprotein (LDL) cholesterol level is a characteristic feature of dyslipidemia in chronic hepatitis C virus (HCV) infection. However, abnormality in serum triglyceride (TG) has not been fully investigated. To clarify the impact of HCV genotype 1b (G1b) infection and advanced fibrosis on serum TG profiles, TG concentrations in lipoprotein fractions were examined in fasting sera from 185 subjects with active or cleared HCV infection by high-performance liquid chromatography. Serum lipoproteins were fractionated into four classes: chylomicron, very low-density lipoprotein (VLDL), LDL, and high-density lipoprotein (HDL). Then, the significance of HCV G1b infection on TG levels in each lipoprotein fraction was determined using multiple regression models. We found that active HCV G1b infection was positively associated with high HDL-TG levels and low VLDL-TG levels, independent of other factors included in the regression model. In VLDL sub-fractions, active HCV infection was only found to be associated with low levels of large VLDL-TG. Similarly, advanced liver fibrosis in chronic HCV G1b infection was associated with high levels of LDL-TG, HDL-TG, and small VLDL-TG, independent of other clinical factors. These findings indicate that active HCV G1b infection and advanced fibrosis are closely associated with abnormal serum TG profiles.

  17. Dinosaurs can fly -- High performance refining

    SciTech Connect

    Treat, J.E.

    1995-09-01

    High performance refining requires that one develop a winning strategy based on a clear understanding of one`s position in one`s company`s value chain; one`s competitive position in the products markets one serves; and the most likely drivers and direction of future market forces. The author discussed all three points, then described measuring performance of the company. To become a true high performance refiner often involves redesigning the organization as well as the business processes. The author discusses such redesigning. The paper summarizes ten rules to follow to achieve high performance: listen to the market; optimize; organize around asset or area teams; trust the operators; stay flexible; source strategically; all maintenance is not equal; energy is not free; build project discipline; and measure and reward performance. The paper then discusses the constraints to the implementation of change.

  18. High Performance Split-Stirling Cooler Program

    DTIC Science & Technology

    1982-09-01

    7 SPLIT- STIRLING CYCLE CRYOCOOLER . ...... . . . . . 13 8 TEMPERATURE-SHOCK COMPARISON PERFORMANCE DATA, S/N 002 . . 23 9 TEMPERATURE-SHOCK...PERFORMANCE SPLIT- STIRLING "COOLER PROGRAM FINAL TECHNICAL REPORT "September 1982 Prepared for NIGHT VISION AND ELECTRO-OPTICS LABORATORI ES "Contract DAAK70...REPORT & P.Vt2OO COVERED HIGH PERFORMANCE SPLIT- STIRLING COOLER PROGRAM Final Technical Sept. 1979. - Sept. 1982 S. PERPORMING ORO. REPORT KUMMER

  19. Architecture Analysis of High Performance Capacitors (POSTPRINT)

    DTIC Science & Technology

    2009-07-01

    includes the measurement of heat dissipated from a recently developed fluorenyl polyester (FPE) capacitor under an AC excitation. II. Capacitor ...AFRL-RZ-WP-TP-2010-2100 ARCHITECTURE ANALYSIS OF HIGH PERFORMANCE CAPACITORS (POSTPRINT) Hiroyuki Kosai and Tyler Bixel UES, Inc...2009 4. TITLE AND SUBTITLE ARCHITECTURE ANALYSIS OF HIGH PERFORMANCE CAPACITORS (POSTPRINT) 5a. CONTRACT NUMBER In-house 5b. GRANT NUMBER 5c

  20. (/sup 3/H)dihydroergotamine as a high-affinity, slowly dissociating radioligand for 5-HT1B binding sites in rat brain membranes: evidence for guanine nucleotide regulation of agonist affinity states

    SciTech Connect

    Hamblin, M.W.; Ariani, K.; Adriaenssens, P.I.; Ciaranello, R.D.

    1987-12-01

    (/sup 3/H)Dihydroergotamine (DE) labels a population of binding sites in rat brain membranes with an affinity of approximately 70 pM in both hippocampus (maximal binding at saturation (Bmax) = 340 fmol/mg of protein) and cerebral cortex (Bmax = 250 fmol/mg of protein). Specific binding typically comprises about 97% of total binding at the Kd of the radioligand when nonspecific binding is determined in the presence of 100 nM unlabeled DE. Association kinetics at 37 degrees C are consistent with a uniform association rate constant for all sites labeled. Specific binding is completely reversible with addition of excess unlabeled DE, but dissociation does not proceed with simple first-order kinetics, suggesting the presence of more than one discrete binding site. Competition studies with selective drugs reveal alpha adrenergic, 5-HT1A and 5-HT1B components of (/sup 3/H)DE specific binding. When phentolamine (500 nM) is included to block alpha receptors and DPAT (100 nM) or spiroxatrine (500 nM) is included to block 5-HT1A receptors, specific binding is exclusively to sites with drug affinities characteristic of 5-HT1B receptors. Under these 5-HT1B-selective conditions, (/sup 3/H)DE binding is about 90% specific, with a Kd of about 50 to 60 pM and a Bmax of 96 fmol/mg of protein in hippocampus and 77 fmol/mg of protein in cortex. (/sup 3/H)DE binding to 5-HT1B sites is very slowly dissociable, with a T1/2 of greater than 2 h at 37 degrees C. 5-HT1B antagonists and DE itself yield competition curves at (/sup 3/H)DE-labeled 5-HT1B sites that are adequately fit assuming a single site in nonlinear regression analysis. Addition of 100 microM guanylyl 5'-imidodiphosphate appears to convert nearly all 5-HT1B sites to those having low affinity for agonists while having a much smaller effect on the binding of (/sup 3/H)DE.

  1. Highlighting High Performance: Whitman Hanson Regional High School; Whitman, Massachusetts

    SciTech Connect

    Not Available

    2006-06-01

    This brochure describes the key high-performance building features of the Whitman-Hanson Regional High School. The brochure was paid for by the Massachusetts Technology Collaborative as part of their Green Schools Initiative. High-performance features described are daylighting and energy-efficient lighting, indoor air quality, solar and wind energy, building envelope, heating and cooling systems, water conservation, and acoustics. Energy cost savings are also discussed.

  2. Influence of the Cyp1B1 L432V gene polymorphism and exposure to tobacco smoke on Cyp1B1 mRNA expression in human leukocytes.

    PubMed

    Helmig, Simone; Hadzaad, Bahar; Döhrel, Juliane; Schneider, Joachim

    2009-07-01

    Cytochrome P450 1B1 (CYP1B1), a phase I enzyme, is involved in the activation of a broad spectrum of procarcinogens. An association of the Cyp1B1 L432V polymorphism with diverse types of cancer, as well as an impact on the catalytic activity of the enzyme, has been described. To show the functional impact of the allelic variant Cyp1B1*3, we investigated the quantitative Cyp1B1 mRNA expression in a population of smokers, nonsmokers, and ex-smokers and determined their genotypes. Detection of the L432V polymorphism in exon 3 of the Cyp1B1 gene was performed by rapid capillary polymerase chain reaction (PCR) with melting curve analysis. For quantitative comparison of Cyp1B1 mRNA levels, real-time PCR was performed using SYBR Green fluorescence in a LightCycler system. Calculations of expression were made with the 2(-DeltaDeltaCT) method. In comparing relative Cyp1B1 mRNA expression, highly significant differences between the two homozygote genotypes *1/*1 and *3/*3 (0.185 +/- 0.027, n = 118 versus 0.071 +/- 0.013, n = 56; p = 0.000), as well as between the heterozygote genotype *1/*3 and the homozygote genotype *3/*3 (0.178 +/- 0.025, n = 171 versus 0.071 +/- 0.013, n = 56; p = 0.000), were revealed. Significant differences between the genotypes were also detected within the subgroups of smokers, nonsmokers, and ex-smokers. No significant differences were determined in comparing the relative Cyp1B1 mRNA expression with regard to tobacco smoke exposure. Our results suggest that genotypes carrying the C allele (*1/*1 and *1/*3) at Cyp1B1 L432V polymorphism have a significantly higher Cyp1B1 mRNA expression compared with the genotype without the C allele (*3/*3). Gene expression of Cyp1B1 mRNA cannot be used as a biomarker for exposure of tobacco smoke.

  3. Overview of high performance aircraft propulsion research

    NASA Technical Reports Server (NTRS)

    Biesiadny, Thomas J.

    1992-01-01

    The overall scope of the NASA Lewis High Performance Aircraft Propulsion Research Program is presented. High performance fighter aircraft of interest include supersonic flights with such capabilities as short take off and vertical landing (STOVL) and/or high maneuverability. The NASA Lewis effort involving STOVL propulsion systems is focused primarily on component-level experimental and analytical research. The high-maneuverability portion of this effort, called the High Alpha Technology Program (HATP), is part of a cooperative program among NASA's Lewis, Langley, Ames, and Dryden facilities. The overall objective of the NASA Inlet Experiments portion of the HATP, which NASA Lewis leads, is to develop and enhance inlet technology that will ensure high performance and stability of the propulsion system during aircraft maneuvers at high angles of attack. To accomplish this objective, both wind-tunnel and flight experiments are used to obtain steady-state and dynamic data, and computational fluid dynamics (CFD) codes are used for analyses. This overview of the High Performance Aircraft Propulsion Research Program includes a sampling of the results obtained thus far and plans for the future.

  4. Teacher Accountability at High Performing Charter Schools

    ERIC Educational Resources Information Center

    Aguirre, Moises G.

    2016-01-01

    This study will examine the teacher accountability and evaluation policies and practices at three high performing charter schools located in San Diego County, California. Charter schools are exempted from many laws, rules, and regulations that apply to traditional school systems. By examining the teacher accountability systems at high performing…

  5. High Performance Work Systems for Online Education

    ERIC Educational Resources Information Center

    Contacos-Sawyer, Jonna; Revels, Mark; Ciampa, Mark

    2010-01-01

    The purpose of this paper is to identify the key elements of a High Performance Work System (HPWS) and explore the possibility of implementation in an online institution of higher learning. With the projected rapid growth of the demand for online education and its importance in post-secondary education, providing high quality curriculum, excellent…

  6. High Performance Work Systems for Online Education

    ERIC Educational Resources Information Center

    Contacos-Sawyer, Jonna; Revels, Mark; Ciampa, Mark

    2010-01-01

    The purpose of this paper is to identify the key elements of a High Performance Work System (HPWS) and explore the possibility of implementation in an online institution of higher learning. With the projected rapid growth of the demand for online education and its importance in post-secondary education, providing high quality curriculum, excellent…

  7. Teacher Accountability at High Performing Charter Schools

    ERIC Educational Resources Information Center

    Aguirre, Moises G.

    2016-01-01

    This study will examine the teacher accountability and evaluation policies and practices at three high performing charter schools located in San Diego County, California. Charter schools are exempted from many laws, rules, and regulations that apply to traditional school systems. By examining the teacher accountability systems at high performing…

  8. Molecular dynamics simulation study of PTP1B with allosteric inhibitor and its application in receptor based pharmacophore modeling

    NASA Astrophysics Data System (ADS)

    Bharatham, Kavitha; Bharatham, Nagakumar; Kwon, Yong Jung; Lee, Keun Woo

    2008-12-01

    Allosteric inhibition of protein tyrosine phosphatase 1B (PTP1B), has paved a new path to design specific inhibitors for PTP1B, which is an important drug target for the treatment of type II diabetes and obesity. The PTP1B1-282-allosteric inhibitor complex crystal structure lacks α7 (287-298) and moreover there is no available 3D structure of PTP1B1-298 in open form. As the interaction between α7 and α6-α3 helices plays a crucial role in allosteric inhibition, α7 was modeled to the PTP1B1-282 in open form complexed with an allosteric inhibitor (compound-2) and a 5 ns MD simulation was performed to investigate the relative orientation of the α7-α6-α3 helices. The simulation conformational space was statistically sampled by clustering analyses. This approach was helpful to reveal certain clues on PTP1B allosteric inhibition. The simulation was also utilized in the generation of receptor based pharmacophore models to include the conformational flexibility of the protein-inhibitor complex. Three cluster representative structures of the highly populated clusters were selected for pharmacophore model generation. The three pharmacophore models were subsequently utilized for screening databases to retrieve molecules containing the features that complement the allosteric site. The retrieved hits were filtered based on certain drug-like properties and molecular docking simulations were performed in two different conformations of protein. Thus, performing MD simulation with α7 to investigate the changes at the allosteric site, then developing receptor based pharmacophore models and finally docking the retrieved hits into two distinct conformations will be a reliable methodology in identifying PTP1B allosteric inhibitors.

  9. Noise of High Performance Aircraft at Afterburner

    DTIC Science & Technology

    2016-02-10

    14,  2015   Submitted  to   The  Office  of  Naval  Research     Project  Title:   Noise  of  High-­‐Performance...Until recently, the noise of high performance military aircraft has not been studied in details. During the last two years, noise spectral data from the...any major differences between the dominant noise components of these jets and those of a standard high temperature laboratory supersonic jet. It is

  10. Oligouridylate Binding Protein 1b Plays an Integral Role in Plant Heat Stress Tolerance

    PubMed Central

    Nguyen, Cam Chau; Nakaminami, Kentaro; Matsui, Akihiro; Kobayashi, Shuhei; Kurihara, Yukio; Toyooka, Kiminori; Tanaka, Maho; Seki, Motoaki

    2016-01-01

    Stress granules (SGs), which are formed in the plant cytoplasm under stress conditions, are transient dynamic sites (particles) for mRNA storage. SGs are actively involved in protecting mRNAs from degradation. Oligouridylate binding protein 1b (UBP1b) is a component of SGs. The formation of microscopically visible cytoplasmic foci, referred to as UBP1b SG, was induced by heat treatment in UBP1b-overexpressing Arabidopsis plants (UBP1b-ox). A detailed understanding of the function of UBP1b, however, is still not clear. UBP1b-ox plants displayed increased heat tolerance, relative to control plants, while ubp1b mutants were more sensitive to heat stress than control plants. Microarray analysis identified 117 genes whose expression was heat-inducible and higher in the UBP1b-ox plants. RNA decay analysis was performed using cordycepin, a transcriptional inhibitor. In order to determine if those genes serve as targets of UBP1b, the rate of RNA degradation of a DnaJ heat shock protein and a stress-associated protein (AtSAP3) in UBP1b-ox plants was slower than in control plants; indicating that the mRNAs of these genes were protected within the UBP1b SG granule. Collectively, these data demonstrate that UBP1b plays an integral role in heat stress tolerance in plants. PMID:27379136

  11. The structure and conformational switching of Rap1B.

    PubMed

    Noguchi, Hiroki; Ikegami, Takahisa; Nagadoi, Aritaka; Kamatari, Yuji O; Park, Sam-Yong; Tame, Jeremy R H; Unzai, Satoru

    2015-06-19

    Rap1B is a small GTPase involved in the regulation of numerous cellular processes including synaptic plasticity, one of the bases of memory. Like other members of the Ras family, the active GTP-bound form of Rap1B can bind to a large number of effector proteins and so transmit signals to downstream components of the signaling pathways. The structure of Rap1B bound only to a nucleotide has yet to be solved, but might help reveal an inactive conformation that can be stabilized by a small molecule drug. Unlike other Ras family proteins such as H-Ras and Rap2A, Rap1B crystallizes in an intermediate state when bound to a non-hydrolyzable GTP analog. Comparison with H-Ras and Rap2A reveals conservative mutations relative to Rap1B, distant from the bound nucleotide, which control how readily the protein may adopt the fully activated form in the presence of GTP. High resolution crystallographic structures of mutant proteins show how these changes may influence the hydrogen bonding patterns of the key switch residues.

  12. Oncolytic Replication of E1b-Deleted Adenoviruses

    PubMed Central

    Cheng, Pei-Hsin; Wechman, Stephen L.; McMasters, Kelly M.; Zhou, Heshan Sam

    2015-01-01

    Various viruses have been studied and developed for oncolytic virotherapies. In virotherapy, a relatively small amount of viruses used in an intratumoral injection preferentially replicate in and lyse cancer cells, leading to the release of amplified viral particles that spread the infection to the surrounding tumor cells and reduce the tumor mass. Adenoviruses (Ads) are most commonly used for oncolytic virotherapy due to their infection efficacy, high titer production, safety, easy genetic modification, and well-studied replication characteristics. Ads with deletion of E1b55K preferentially replicate in and destroy cancer cells and have been used in multiple clinical trials. H101, one of the E1b55K-deleted Ads, has been used for the treatment of late-stage cancers as the first approved virotherapy agent. However, the mechanism of selective replication of E1b-deleted Ads in cancer cells is still not well characterized. This review will focus on three potential molecular mechanisms of oncolytic replication of E1b55K-deleted Ads. These mechanisms are based upon the functions of the viral E1B55K protein that are associated with p53 inhibition, late viral mRNA export, and cell cycle disruption. PMID:26561828

  13. Fragile X mental retardation protein interactions with the microtubule associated protein 1B RNA.

    PubMed

    Menon, Lakshmi; Mader, Samantha Ann; Mihailescu, Mihaela-Rita

    2008-08-01

    Fragile X mental retardation syndrome, the most common form of inherited mental retardation, is caused by the absence of the fragile X mental retardation protein (FMRP). FMRP has been shown to use its arginine-glycine-glycine (RGG) box to bind to a subset of RNA targets that form a G quadruplex structure. We performed a detailed analysis of the interactions between the FMRP RGG box and the microtubule associated protein 1B (MAP1B) mRNA, a relevant in vivo FMRP target. We show that MAP1B RNA forms an intramolecular G quadruplex structure, which is bound with high affinity and specificity by the FMRP RGG box. We determined that hydrophobic interactions are important in the FMRP RGG box-MAP1B RNA association, with minor contributions from electrostatic interactions. Our findings that at low protein:RNA ratios the RNA G quadruplex structure is slightly stabilized, whereas at high ratios is unfolded, suggest a mechanism by which the FMRP concentration variation in response to a neurotransmitter stimulation event could act as a regulatory switch for the protein function, from translation repressor to translation activator.

  14. Improved High-Quality Draft Genome Sequence of the Eurypsychrophile Rhodotorula sp. JG1b, Isolated from Permafrost in the Hyperarid Upper-Elevation McMurdo Dry Valleys, Antarctica

    PubMed Central

    Goordial, Jacqueline; Raymond-Bouchard, Isabelle; Riley, Robert; Ronholm, Jennifer; Shapiro, Nicole; Woyke, Tanja; LaButti, Kurt M.; Tice, Hope; Amirebrahimi, Mojgan; Grigoriev, Igor V.; Greer, Charles; Bakermans, Corien

    2016-01-01

    Here, we report the draft genome sequence of Rhodotorula sp. strain JG1b, a yeast that was isolated from ice-cemented permafrost in the upper-elevation McMurdo Dry Valleys, Antarctica. The sequenced genome size is 19.39 Mb, consisting of 156 scaffolds and containing a total of 5,625 predicted genes. This is the first known cold-adapted Rhodotorula sp. sequenced to date. PMID:26988035

  15. Improved High-Quality Draft Genome Sequence of the Eurypsychrophile Rhodotorula sp. JG1b, Isolated from Permafrost in the Hyperarid Upper-Elevation McMurdo Dry Valleys, Antarctica

    DOE PAGES

    Goordial, Jacqueline; Raymond-Bouchard, Isabelle; Riley, Robert; ...

    2016-03-17

    Here, we report the draft genome sequence of Rhodotorula sp. strain JG1b, a yeast that was isolated from ice-cemented permafrost in the upper-elevation McMurdo Dry Valleys, Antarctica. The sequenced genome size is 19.39 Mb, consisting of 156 scaffolds and containing a total of 5,625 predicted genes. This is the first known cold-adapted Rhodotorula sp. sequenced to date.

  16. Improved High-Quality Draft Genome Sequence of the Eurypsychrophile Rhodotorula sp. JG1b, Isolated from Permafrost in the Hyperarid Upper-Elevation McMurdo Dry Valleys, Antarctica

    SciTech Connect

    Goordial, Jacqueline; Raymond-Bouchard, Isabelle; Riley, Robert; Ronholm, Jennifer; Shapiro, Nicole; Woyke, Tanja; LaButti, Kurt M.; Tice, Hope; Amirebrahimi, Mojgan; Grigoriev, Igor V.; Greer, Charles; Bakermans, Corien; Whyte, Lyle

    2016-03-17

    Here, we report the draft genome sequence of Rhodotorula sp. strain JG1b, a yeast that was isolated from ice-cemented permafrost in the upper-elevation McMurdo Dry Valleys, Antarctica. The sequenced genome size is 19.39 Mb, consisting of 156 scaffolds and containing a total of 5,625 predicted genes. This is the first known cold-adapted Rhodotorula sp. sequenced to date.

  17. 7 CFR 1b.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.1 Purpose. (a) This part supplements the regulations for implementation of the National Environmental Policy Act (NEPA), for which regulations were published by the Council on Environmental Quality (CEQ) in 40 CFR parts 1500 through 1508. This...

  18. Experimental Reproduction of Type 1B Chondrules

    NASA Technical Reports Server (NTRS)

    Lofgren, G. E.; Le, L.

    2002-01-01

    We have replicated type 1B chondrule textures and compositions with crystallization experiments in which UOC material was melted at 1400 deg.C and cooled at 5-1000 deg.C/hr using graphite crucibles in evacuated silica tubes to provide a reducing environment. Additional information is contained in the original extended abstract.

  19. 7 CFR 1b.1 - Purpose.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.1 Purpose. (a) This part supplements the regulations for implementation of the National Environmental Policy Act (NEPA), for which regulations were published by the Council on Environmental Quality (CEQ) in 40 CFR parts 1500 through 1508. This part...

  20. 7 CFR 1b.3 - Categorical exclusions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Office of the Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.3 Categorical exclusions... environmental assessment (EA's) or environmental impact statement (EIS's), unless individual agency procedures prescribed otherwise. (1) Policy development, planning and implementation which relate to routine activities...

  1. 7 CFR 1b.3 - Categorical exclusions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Office of the Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.3 Categorical exclusions... environmental assessment (EA's) or environmental impact statement (EIS's), unless individual agency procedures prescribed otherwise. (1) Policy development, planning and implementation which relate to routine activities...

  2. 7 CFR 1b.3 - Categorical exclusions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Office of the Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.3 Categorical exclusions... environmental assessment (EA's) or environmental impact statement (EIS's), unless individual agency procedures prescribed otherwise. (1) Policy development, planning and implementation which relate to routine activities...

  3. 7 CFR 1b.1 - Purpose.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.1 Purpose. (a) This part supplements the regulations for implementation of the National Environmental Policy Act (NEPA), for which regulations were published by the Council on Environmental Quality (CEQ) in 40 CFR parts 1500 through 1508. This part...

  4. 7 CFR 1b.1 - Purpose.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.1 Purpose. (a) This part supplements the regulations for implementation of the National Environmental Policy Act (NEPA), for which regulations were published by the Council on Environmental Quality (CEQ) in 40 CFR parts 1500 through 1508. This part...

  5. 7 CFR 1b.3 - Categorical exclusions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Office of the Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.3 Categorical exclusions... environmental assessment (EA's) or environmental impact statement (EIS's), unless individual agency procedures prescribed otherwise. (1) Policy development, planning and implementation which relate to routine activities...

  6. 7 CFR 1b.1 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Secretary of Agriculture NATIONAL ENVIRONMENTAL POLICY ACT § 1b.1 Purpose. (a) This part supplements the regulations for implementation of the National Environmental Policy Act (NEPA), for which regulations were published by the Council on Environmental Quality (CEQ) in 40 CFR parts 1500 through 1508. This part...

  7. High-performance computing and communications

    SciTech Connect

    Stevens, R.

    1993-11-01

    This presentation has two parts. The first part discusses the US High-Performance Computing and Communications program -- its goals, funding, process, revisions, and research in high-performance computing systems, advanced software technology, and basic research and human resources. The second part of the presentation covers specific work conducted under this program at Argonne National Laboratory. Argonne`s efforts focus on computational science research, software tool development, and evaluation of experimental computer architectures. In addition, the author describes collaborative activities at Argonne in high-performance computing, including an Argonne/IBM project to evaluate and test IBM`s newest parallel computers and the Scalable I/O Initiative being spearheaded by the Concurrent Supercomputing Consortium.

  8. High performance bio-integrated devices

    NASA Astrophysics Data System (ADS)

    Kim, Dae-Hyeong; Lee, Jongha; Park, Minjoon

    2014-06-01

    In recent years, personalized electronics for medical applications, particularly, have attracted much attention with the rise of smartphones because the coupling of such devices and smartphones enables the continuous health-monitoring in patients' daily life. Especially, it is expected that the high performance biomedical electronics integrated with the human body can open new opportunities in the ubiquitous healthcare. However, the mechanical and geometrical constraints inherent in all standard forms of high performance rigid wafer-based electronics raise unique integration challenges with biotic entities. Here, we describe materials and design constructs for high performance skin-mountable bio-integrated electronic devices, which incorporate arrays of single crystalline inorganic nanomembranes. The resulting electronic devices include flexible and stretchable electrophysiology electrodes and sensors coupled with active electronic components. These advances in bio-integrated systems create new directions in the personalized health monitoring and/or human-machine interfaces.

  9. Engineering high-performance vertical cavity lasers

    SciTech Connect

    Lear, K.L.; Hou, H.Q.; Hietala, V.M.; Choquette, K.D.; Schneider, R.P. Jr.

    1996-12-31

    The cw and high-speed performance of vertical cavity surface emitting laser diodes (VCSELs) are affected by both electrical and optical issues arising from the geometry and fabrication of these devices. Structures with low resistance semiconductor mirrors and Al-oxide confinement layers address these issues and have produced record performance including 50% power conversion efficiency and modulation bandwidths up to 20 GHz at small bias currents.

  10. Massive Contingency Analysis with High Performance Computing

    SciTech Connect

    Huang, Zhenyu; Chen, Yousu; Nieplocha, Jaroslaw

    2009-07-26

    Contingency analysis is a key function in the Energy Management System (EMS) to assess the impact of various combinations of power system component failures based on state estimates. Contingency analysis is also extensively used in power market operation for feasibility test of market solutions. Faster analysis of more cases is required to safely and reliably operate today’s power grids with less marginal and more intermittent renewable energy sources. Enabled by the latest development in the computer industry, high performance computing holds the promise of meet the need in the power industry. This paper investigates the potential of high performance computing for massive contingency analysis. The framework of "N-x" contingency analysis is established and computational load balancing schemes are studied and implemented with high performance computers. Case studies of massive 300,000-contingency-case analysis using the Western Electricity Coordinating Council power grid model are presented to illustrate the application of high performance computing and demonstrate the performance of the framework and computational load balancing schemes.

  11. Achieving High Performance Perovskite Solar Cells

    NASA Astrophysics Data System (ADS)

    Yang, Yang

    2015-03-01

    Recently, metal halide perovskite based solar cell with the characteristics of rather low raw materials cost, great potential for simple process and scalable production, and extreme high power conversion efficiency (PCE), have been highlighted as one of the most competitive technologies for next generation thin film photovoltaic (PV). In UCLA, we have realized an efficient pathway to achieve high performance pervoskite solar cells, where the findings are beneficial to this unique materials/devices system. Our recent progress lies in perovskite film formation, defect passivation, transport materials design, interface engineering with respect to high performance solar cell, as well as the exploration of its applications beyond photovoltaics. These achievements include: 1) development of vapor assisted solution process (VASP) and moisture assisted solution process, which produces perovskite film with improved conformity, high crystallinity, reduced recombination rate, and the resulting high performance; 2) examination of the defects property of perovskite materials, and demonstration of a self-induced passivation approach to reduce carrier recombination; 3) interface engineering based on design of the carrier transport materials and the electrodes, in combination with high quality perovskite film, which delivers 15 ~ 20% PCEs; 4) a novel integration of bulk heterojunction to perovskite solar cell to achieve better light harvest; 5) fabrication of inverted solar cell device with high efficiency and flexibility and 6) exploration the application of perovskite materials to photodetector. Further development in film, device architecture, and interfaces will lead to continuous improved perovskite solar cells and other organic-inorganic hybrid optoelectronics.

  12. Performance variability of highly parallel architectures

    SciTech Connect

    Kramer, William T.C.; Ryan, Clint

    2003-05-01

    The design and evaluation of high performance computers has concentrated on increasing computational speed for applications. This performance is often measured on a well configured dedicated system to show the best case. In the real environment, resources are not always dedicated to a single task, and systems run tasks that may influence each other, so run times vary, sometimes to an unreasonably large extent. This paper explores the amount of variation seen across four large distributed memory systems in a systematic manner. It then analyzes the causes for the variations seen and discusses what can be done to decrease the variation without impacting performance.

  13. Role of CYP1B1 in PAH-DNA Adduct Formation and Breast Cancer Risk

    DTIC Science & Technology

    2006-03-01

    xenobiotic metabolism, CYP1B1 , gene expression, genetic polymorphism , DNA adducts 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT 18...Task 2: Characterize the breast tissue samples in respect to CYP1B1 expression and CYP1B1 polymorphism – in progress a. Isolate DNA and RNA from...19.78) 0.06 – 73.7 d. Perform CYP1B1 genotype analysis The CYP1B1 genotype at two polymorphic sites located in the catalytic side of the enzyme

  14. High Performance Multiwall Carbon Nanotube Bolometers

    DTIC Science & Technology

    2010-10-21

    REPORT High performance multiwall carbon nanotube bolometers 14. ABSTRACT 16. SECURITY CLASSIFICATION OF: High infrared bolometric photoresponse has...been observed in multiwall carbon nanotube MWCNT films at room temperature. The observed detectivity D in exceeding 3.3 106 cm Hz1/2 /W on MWCNT film...U.S. Army Research Office P.O. Box 12211 Research Triangle Park, NC 27709-2211 15. SUBJECT TERMS carbon nanotube, infrared detector, bolometer

  15. Performance analysis of memory hierachies in high performance systems

    SciTech Connect

    Yogesh, Agrawel

    1993-07-01

    This thesis studies memory bandwidth as a performance predictor of programs. The focus of this work is on computationally intensive programs. These programs are the most likely to access large amounts of data, stressing the memory system. Computationally intensive programs are also likely to use highly optimizing compilers to produce the fastest executables possible. Methods to reduce the amount of data traffic by increasing the average number of references to each item while it resides in the cache are explored. Increasing the average number of references to each cache item reduces the number of memory requests. Chapter 2 describes the DLX architecture. This is the architecture on which all the experiments were performed. Chapter 3 studies memory moves as a performance predictor for a group of application programs. Chapter 4 introduces a model to study the performance of programs in the presence of memory hierarchies. Chapter 5 explores some compiler optimizations that can help increase the references to each item while it resides in the cache.

  16. A new release 06 of the GRACE Atmosphere and Ocean De-aliasing Level-1B (AOD1B) product

    NASA Astrophysics Data System (ADS)

    Fagiolini, E.; Dobslaw, H.; Flechtner, F.; Rudenko, S.

    2014-12-01

    We present a new release 06 of the GRACE AOD1B product, intended to serve as a background model for the removal of high-frequency non-tidal mass variations due to short-term (daily and sub-daily) mass transport in the atmosphere and oceans. AOD1B shall avoid aliasing of these high frequency signals into monthly gravity models derived from modern gravity missions (CHAMP, GRACE or GRACE-FO) and shall help to consistently reprocess altimetry and SLR satellites for later combination of gravimetric and geometric results. Therefore, AOD1B RL06 will be provided for an extended time-range (from 1976 till present). Some results on the use of the new and previous releases of the AOD1B product for precise orbit determination (POD) of these satellites are presented as well. For AOD1B RL06 the ocean part is the same as in RL05, while the atmospheric one includes an improved algorithm for the vertical integration of the atmospheric columns as well as a correction strategy for errors (jumps) present in the atmospheric input data. These errors are estimated by comparing the standard atmospheric coefficients based on the ECMWF operational analysis with independently generated coefficients based on the ECMWF ERA-Interim reanalysis. With this, we are able to correct the 6-hourly AOD1B data sets applied during GRACE POD. The derived gravity solutions, as well as the monthly averages 'GAA' (atmosphere only) and 'GAC' (atmosphere combined with ocean), are then free from errors present in the atmospheric input data. Furthermore, we are checking routinely processing changes at ECMWF, since they are strictly associated with the above mentioned errors, and will estimate new correction coefficients, if necessary.

  17. Strategy Guideline: Partnering for High Performance Homes

    SciTech Connect

    Prahl, D.

    2013-01-01

    High performance houses require a high degree of coordination and have significant interdependencies between various systems in order to perform properly, meet customer expectations, and minimize risks for the builder. Responsibility for the key performance attributes is shared across the project team and can be well coordinated through advanced partnering strategies. For high performance homes, traditional partnerships need to be matured to the next level and be expanded to all members of the project team including trades, suppliers, manufacturers, HERS raters, designers, architects, and building officials as appropriate. In an environment where the builder is the only source of communication between trades and consultants and where relationships are, in general, adversarial as opposed to cooperative, the chances of any one building system to fail are greater. Furthermore, it is much harder for the builder to identify and capitalize on synergistic opportunities. Partnering can help bridge the cross-functional aspects of the systems approach and achieve performance-based criteria. Critical success factors for partnering include support from top management, mutual trust, effective and open communication, effective coordination around common goals, team building, appropriate use of an outside facilitator, a partnering charter progress toward common goals, an effective problem-solving process, long-term commitment, continuous improvement, and a positive experience for all involved.

  18. The ganglioside GQ1b regulates BDNF expression via the NMDA receptor signaling pathway.

    PubMed

    Shin, Min Kyoo; Jung, Woo Ram; Kim, Hong Gi; Roh, Seung Eon; Kwak, Choong Hwan; Kim, Cheorl Ho; Kim, Sang Jeong; Kim, Kil Lyong

    2014-02-01

    Gangliosides are sialic acid-containing glycosphingolipids which play a role in neuronal functions. Among the gangliosides, tetrasialoganglioside GQ1b shows neurotrophic factor-like actions, such as increasing neurite outgrowth, cell proliferation, and long-term potentiation. In addition, we recently reported that GQ1b improves spatial learning and memory performance in naïve rats. However, it is still unknown how GQ1b exerts its diverse neuronal functions. Thus, we hypothesized that GQ1b might influence synaptic activity by regulating brain-derived neurotrophic factor (BDNF) expression, which is an important protein for synaptic plasticity and cognition. Interestingly, GQ1b treatment increased BDNF expression in GQ1b-null SH-SY5Y cell lines and rat primary cortical neurons. Additionally, we confirmed whether the observed effects were due to GQ1b or due to a ganglioside with fewer sialic acid molecules (GT1b and GD1b) created by the sialidases present on the plasma membranes, by directly applying GT1b and GD1b or GQ1b co-treated with a sialidase inhibitor. Treatment with GT1b or GD1b had no effect on BDNF expression, whereas co-treatment with a sialidase inhibitor and GQ1b significantly increased BDNF levels. Moreover, GQ1b restored the decreased BDNF expression induced by the ganglioside synthesis inhibitor, D-PDMP, in rat primary cortical neurons. GQ1b treatment significantly increased BDNF levels, whereas pretreatment with the N-methyl-d-aspartate (NMDA) receptor antagonist D-AP5 blocked the effects of GQ1b on BDNF expression, suggesting that GQ1b regulates BDNF expression via the NMDA receptor signaling. Finally, we performed an intracerebroventricular GQ1b injection, which resulted in increased prefrontal and hippocampal BDNF expression in vivo. These findings demonstrate, for the first time, that tetrasialoganglioside GQ1b regulates BDNF expression in vitro and in vivo. Copyright © 2013 Elsevier Ltd. All rights reserved.

  19. Project materials [Commercial High Performance Buildings Project

    SciTech Connect

    2001-01-01

    The Consortium for High Performance Buildings (ChiPB) is an outgrowth of DOE'S Commercial Whole Buildings Roadmapping initiatives. It is a team-driven public/private partnership that seeks to enable and demonstrate the benefit of buildings that are designed, built and operated to be energy efficient, environmentally sustainable, superior quality, and cost effective.

  20. High Performance Computing and Communications Panel Report.

    ERIC Educational Resources Information Center

    President's Council of Advisors on Science and Technology, Washington, DC.

    This report offers advice on the strengths and weaknesses of the High Performance Computing and Communications (HPCC) initiative, one of five presidential initiatives launched in 1992 and coordinated by the Federal Coordinating Council for Science, Engineering, and Technology. The HPCC program has the following objectives: (1) to extend U.S.…

  1. Commercial Buildings High Performance Rooftop Unit Challenge

    SciTech Connect

    2011-12-16

    The U.S. Department of Energy (DOE) and the Commercial Building Energy Alliances (CBEAs) are releasing a new design specification for high performance rooftop air conditioning units (RTUs). Manufacturers who develop RTUs based on this new specification will find strong interest from the commercial sector due to the energy and financial savings.

  2. Creating a High-Performance School System.

    ERIC Educational Resources Information Center

    Thompson, Scott

    2003-01-01

    Describes several critical factors of a high-performing school system such as the system holds itself accountable for the success of all its schools. Provides school district examples of critical success factors in action. Includes districts in Colorado, Washington, Texas, California, New Jersey. Discusses the role of strategic and authentic…

  3. Technology Leadership in Malaysia's High Performance School

    ERIC Educational Resources Information Center

    Yieng, Wong Ai; Daud, Khadijah Binti

    2017-01-01

    Headmaster as leader of the school also plays a role as a technology leader. This applies to the high performance schools (HPS) headmaster as well. The HPS excel in all aspects of education. In this study, researcher is interested in examining the role of the headmaster as a technology leader through interviews with three headmasters of high…

  4. Task parallelism and high-performance languages

    SciTech Connect

    Foster, I.

    1996-03-01

    The definition of High Performance Fortran (HPF) is a significant event in the maturation of parallel computing: it represents the first parallel language that has gained widespread support from vendors and users. The subject of this paper is to incorporate support for task parallelism. The term task parallelism refers to the explicit creation of multiple threads of control, or tasks, which synchronize and communicate under programmer control. Task and data parallelism are complementary rather than competing programming models. While task parallelism is more general and can be used to implement algorithms that are not amenable to data-parallel solutions, many problems can benefit from a mixed approach, with for example a task-parallel coordination layer integrating multiple data-parallel computations. Other problems admit to both data- and task-parallel solutions, with the better solution depending on machine characteristics, compiler performance, or personal taste. For these reasons, we believe that a general-purpose high-performance language should integrate both task- and data-parallel constructs. The challenge is to do so in a way that provides the expressivity needed for applications, while preserving the flexibility and portability of a high-level language. In this paper, we examine and illustrate the considerations that motivate the use of task parallelism. We also describe one particular approach to task parallelism in Fortran, namely the Fortran M extensions. Finally, we contrast Fortran M with other proposed approaches and discuss the implications of this work for task parallelism and high-performance languages.

  5. High Performance Builder Spotlight: Imagine Homes

    SciTech Connect

    2011-01-01

    Imagine Homes, working with the DOE's Building America research team member IBACOS, has developed a system that can be replicated by other contractors to build affordable, high-performance homes. Imagine Homes has used the system to produce more than 70 Builders Challenge-certified homes per year in San Antonio over the past five years.

  6. Miniaturized high-performance MEMS accelerometer detector

    NASA Astrophysics Data System (ADS)

    Gonseth, Stephan; Rudolf, Felix; Eichenberger, Christoph; Durrant, Dick; Airey, Phil

    2015-06-01

    In the framework of the demonstration of European capabilities for future space exploration mission, a high-performance miniaturized MEMS accelerometer detector is developed by Colibrys for incorporation into a compact inertial measurement unit (IMU). The envisaged missions where a miniaturized IMU is under development by SEA should cover: Aerobraking;

  7. High Performance Work Organizations. Myths and Realities.

    ERIC Educational Resources Information Center

    Kerka, Sandra

    Organizations are being urged to become "high performance work organizations" (HPWOs) and vocational teachers have begun considering how best to prepare workers for them. Little consensus exists as to what HPWOs are. Several common characteristics of HPWOs have been identified, and two distinct models of HPWOs are emerging in the United…

  8. Supervising the highly performing general practice registrar.

    PubMed

    Morgan, Simon

    2014-02-01

    There is extensive literature on the poorly performing learner. In contrast, there is very little written on supervising the highly performing registrar. Outstanding trainees with high-level knowledge and skills can be a challenge for supervisors to supervise and teach. Narrative review and discussion. As with all learners, a learning-needs analysis is fundamental to successful supervision. The key to effective teaching of the highly performing registrar is to contextualise clinical knowledge and skills with the wisdom of accumulated experience. Moreover, supervisors must provide a stimulating learning environment, with regular opportunities for intellectual challenge. The provision of specific, constructive feedback is essential. There are potential opportunities to extend the highly performing registrar in all domains of general practice, namely communication skills and patient-centred care, applied knowledge and skills, population health, professionalism, and organisation and legal issues. Specific teaching strategies include role-play, video-consultation review, random case analysis, posing hypothetical clinical scenarios, role modelling and teaching other learners. © 2014 John Wiley & Sons Ltd.

  9. Debugging a high performance computing program

    DOEpatents

    Gooding, Thomas M.

    2013-08-20

    Methods, apparatus, and computer program products are disclosed for debugging a high performance computing program by gathering lists of addresses of calling instructions for a plurality of threads of execution of the program, assigning the threads to groups in dependence upon the addresses, and displaying the groups to identify defective threads.

  10. Debugging a high performance computing program

    DOEpatents

    Gooding, Thomas M.

    2014-08-19

    Methods, apparatus, and computer program products are disclosed for debugging a high performance computing program by gathering lists of addresses of calling instructions for a plurality of threads of execution of the program, assigning the threads to groups in dependence upon the addresses, and displaying the groups to identify defective threads.

  11. High-Performance, Low Environmental Impact Refrigerants

    NASA Technical Reports Server (NTRS)

    McCullough, E. T.; Dhooge, P. M.; Glass, S. M.; Nimitz, J. S.

    2001-01-01

    Refrigerants used in process and facilities systems in the US include R-12, R-22, R-123, R-134a, R-404A, R-410A, R-500, and R-502. All but R-134a, R-404A, and R-410A contain ozone-depleting substances that will be phased out under the Montreal Protocol. Some of the substitutes do not perform as well as the refrigerants they are replacing, require new equipment, and have relatively high global warming potentials (GWPs). New refrigerants are needed that addresses environmental, safety, and performance issues simultaneously. In efforts sponsored by Ikon Corporation, NASA Kennedy Space Center (KSC), and the US Environmental Protection Agency (EPA), ETEC has developed and tested a new class of refrigerants, the Ikon (registered) refrigerants, based on iodofluorocarbons (IFCs). These refrigerants are nonflammable, have essentially zero ozone-depletion potential (ODP), low GWP, high performance (energy efficiency and capacity), and can be dropped into much existing equipment.

  12. High performance adaptive tracking system: HPATS

    NASA Astrophysics Data System (ADS)

    Downs, James; Cannon, Randy; Segewitz, Markus; Stockum, Larry

    2005-05-01

    A high performance tracking system that adaptively adjusts the tracker algorithms and track loop parameters based on real-time scene statistics has been developed and demonstrated against realistic target scenarios. The HPATS provides the capability to acquire and track very low contrast targets in the presence of background clutter and time-vaying target conditions to sub-pixel accuracy. HPATS is applicable to both fire control and terminal guidance applications that incorporate imaging sensors. An overview of the tracking system design, simulation modeling, tracker metrics tools, and field test examples of low contrast target tracking performance is presented. The HPATS technology development included a high fidelity Integrated Flight Simulation (IFS) that modeled the end to end performance of the missile fly out, the target acquisition, the target tracking, aim-point selection, terminal guidance, and lethality.

  13. High performance flight simulation at NASA Langley

    NASA Technical Reports Server (NTRS)

    Cleveland, Jeff I., II; Sudik, Steven J.; Grove, Randall D.

    1992-01-01

    The use of real-time simulation at the NASA facility is reviewed specifically with regard to hardware, software, and the use of a fiberoptic-based digital simulation network. The network hardware includes supercomputers that support 32- and 64-bit scalar, vector, and parallel processing technologies. The software include drivers, real-time supervisors, and routines for site-configuration management and scheduling. Performance specifications include: (1) benchmark solution at 165 sec for a single CPU; (2) a transfer rate of 24 million bits/s; and (3) time-critical system responsiveness of less than 35 msec. Simulation applications include the Differential Maneuvering Simulator, Transport Systems Research Vehicle simulations, and the Visual Motion Simulator. NASA is shown to be in the final stages of developing a high-performance computing system for the real-time simulation of complex high-performance aircraft.

  14. Strategy Guideline. High Performance Residential Lighting

    SciTech Connect

    Holton, J.

    2012-02-01

    This report has been developed to provide a tool for the understanding and application of high performance lighting in the home. The strategies featured in this guide are drawn from recent advances in commercial lighting for application to typical spaces found in residential buildings. This guide offers strategies to greatly reduce lighting energy use through the application of high quality fluorescent and light emitting diode (LED) technologies. It is important to note that these strategies not only save energy in the home but also serve to satisfy the homeowner’s expectations for high quality lighting.

  15. High Performance Woven Mesh Heat Exchangers

    NASA Astrophysics Data System (ADS)

    Wirtz, Richard A.; Li, Chen; Park, Ji-Wook; Xu, Jun

    2002-07-01

    Simple-to-fabricate woven mesh structures, consisting of bonded laminates of two-dimensional plain-weave conductive screens, or three-dimensional orthogonal weaves are described. Geometric equations show that these porous matrices can be fabricated to have a wide range of porosity and a highly anisotropic thermal conductivity vector. A mathematical model of the thermal performance of such a mesh, deployed as a heat exchange surface, is developed. Measurements of pressure drop and overall heat transfer rate are reported and used with the performance model to develop correlation equations of mesh friction factor and Colburn j-factor as a function of coolant properties, mesh characteristics and flow rate through the mesh. A heat exchanger performance analysis delineates conditions where the two mesh technologies offer superior performance.

  16. High voltage electric substation performance in earthquakes

    SciTech Connect

    Eidinger, J.; Ostrom, D.; Matsuda, E.

    1995-12-31

    This paper examines the performance of several types of high voltage substation equipment in past earthquakes. Damage data is provided in chart form. This data is then developed into a tool for estimating the performance of a substation subjected to an earthquake. First, suggests are made about the development of equipment class fragility curves that represent the expected earthquake performance of different voltages and types of equipment. Second, suggestions are made about how damage to individual pieces of equipment at a substation likely affects the post-earthquake performance of the substation as a whole. Finally, estimates are provided as to how quickly a substation, at various levels of damage, can be restored to operational service after the earthquake.

  17. Failure analysis of high performance ballistic fibers

    NASA Astrophysics Data System (ADS)

    Spatola, Jennifer S.

    High performance fibers have a high tensile strength and modulus, good wear resistance, and a low density, making them ideal for applications in ballistic impact resistance, such as body armor. However, the observed ballistic performance of these fibers is much lower than the predicted values. Since the predictions assume only tensile stress failure, it is safe to assume that the stress state is affecting fiber performance. The purpose of this research was to determine if there are failure mode changes in the fiber fracture when transversely loaded by indenters of different shapes. An experimental design mimicking transverse impact was used to determine any such effects. Three different indenters were used: round, FSP, and razor blade. The indenter height was changed to change the angle of failure tested. Five high performance fibers were examined: KevlarRTM KM2, SpectraRTM 130d, DyneemaRTM SK-62 and SK-76, and ZylonRTM 555. Failed fibers were analyzed using an SEM to determine failure mechanisms. The results show that the round and razor blade indenters produced a constant failure strain, as well as failure mechanisms independent of testing angle. The FSP indenter produced a decrease in failure strain as the angle increased. Fibrillation was the dominant failure mechanism at all angles for the round indenter, while through thickness shearing was the failure mechanism for the razor blade. The FSP indenter showed a transition from fibrillation at low angles to through thickness shearing at high angles, indicating that the round and razor blade indenters are extreme cases of the FSP indenter. The failure mechanisms observed with the FSP indenter at various angles correlated with the experimental strain data obtained during fiber testing. This indicates that geometry of the indenter tip in compression is a contributing factor in lowering the failure strain of the high performance fibers. TEM analysis of the fiber failure mechanisms was also attempted, though without

  18. PTP1B inhibitors from Saussrurea lappa.

    PubMed

    Li, S; An, T-Y; Li, J; Shen, Q; Lou, F-C; Hu, L-H

    2006-01-01

    A new lignan glycoside, named 1,5-dihydroxypinoresinol-4'-O-beta-d-glucopyranoside (1), has been isolated from the EtOH extract of the roots of Saussurea lappa, together with twenty known compounds: (+)-1-hydroxypinoresinol-1-O-beta-D-glucopyranoside (2), fraxiresinol-4'-O-beta-D-glucopyranoside (3), (-)-olivil-4''-O-beta-D-glucopyranoside (4), 4-allyl-2,6-dimethoxybenzene-1-O-beta-D-glucopyranoside, syringin, costunolide-15-O-beta-D-glucopyranoside, chlorogenic acid, aloe-emodin-8-O-beta-D-glucopyranoside (5), rhein-8-O-beta-D-glucopyranoside (6), chrysophanol (7), emodin, dehydrocostus lactone, costunolide, beta-costic acid, reynosin, arbusculin A, alpha-cyclocostunolide, beta-cyclocostunolide, santamarine and magnolialide. Three anthraquinones (5-7) showed moderate bioactivity against human Protein Tyrosine Phosphatase 1B (hPTP1B) in vitro.

  19. Effect of the strain-induced melt activation (SIMA) process on the tensile properties of a new developed super high strength aluminum alloy modified by Al-5Ti-1B grain refiner

    SciTech Connect

    Haghparast, Amin; Nourimotlagh, Masoud; Alipour, Mohammad

    2012-09-15

    In this study, the effect of Al-5Ti-1B grain refiners and modified strain-induced melt activation process on an Al-Zn-Mg-Cu alloy was studied. The optimum level of Ti was found to be 0.1 wt.%. The specimens subjected to deformation ratio of 40% (at 300 Degree-Sign C) and various heat treatment times (10-40 min) and temperature (550-600 Degree-Sign C) regimes were characterized in this study. Reheating condition to obtain a fine globular microstructure was optimized. Microstructural examinations were conducted by optical and scanning electron microscopy coupled with an energy dispersive spectrometry. The optimum temperature and time in strain-induced melt activation process are 575 Degree-Sign C and 20 min, respectively. T6 heat treatment including quenching to room temperature and aging at 120 Degree-Sign C for 24 h was employed to reach to the maximum strength. Significant improvements in mechanical properties were obtained with the addition of grain refiner combined with T6 heat treatment. After the T6 heat treatment, the average tensile strength increased from 283 MPa to 587 and 332 MPa to 617 for samples refined with 2 wt.% Al-5Ti-1B before and after strain-induced melt activation process and extrusion process, respectively. Ultimate strength of Ti-refined specimens without SIMA process has a lower value than globular microstructure specimens after SIMA and extrusion process. - Highlights: Black-Right-Pointing-Pointer The effect of Al-5Ti-1B on the aluminum alloy produced by SIMA process was studied. Black-Right-Pointing-Pointer Al-5Ti-1B is an effective in reducing the grain and reagent fine microstructure. Black-Right-Pointing-Pointer Reheating condition to obtain a fine globular microstructure was optimized. Black-Right-Pointing-Pointer The optimum temperature and time in SIMA process are 575 Degree-Sign C and 20 min respectively. Black-Right-Pointing-Pointer UTS of globular structure specimens have a more value than Ti-refined specimens.

  20. High performance anode for advanced Li batteries

    SciTech Connect

    Lake, Carla

    2015-11-02

    The overall objective of this Phase I SBIR effort was to advance the manufacturing technology for ASI’s Si-CNF high-performance anode by creating a framework for large volume production and utilization of low-cost Si-coated carbon nanofibers (Si-CNF) for the battery industry. This project explores the use of nano-structured silicon which is deposited on a nano-scale carbon filament to achieve the benefits of high cycle life and high charge capacity without the consequent fading of, or failure in the capacity resulting from stress-induced fracturing of the Si particles and de-coupling from the electrode. ASI’s patented coating process distinguishes itself from others, in that it is highly reproducible, readily scalable and results in a Si-CNF composite structure containing 25-30% silicon, with a compositionally graded interface at the Si-CNF interface that significantly improve cycling stability and enhances adhesion of silicon to the carbon fiber support. In Phase I, the team demonstrated the production of the Si-CNF anode material can successfully be transitioned from a static bench-scale reactor into a fluidized bed reactor. In addition, ASI made significant progress in the development of low cost, quick testing methods which can be performed on silicon coated CNFs as a means of quality control. To date, weight change, density, and cycling performance were the key metrics used to validate the high performance anode material. Under this effort, ASI made strides to establish a quality control protocol for the large volume production of Si-CNFs and has identified several key technical thrusts for future work. Using the results of this Phase I effort as a foundation, ASI has defined a path forward to commercialize and deliver high volume and low-cost production of SI-CNF material for anodes in Li-ion batteries.

  1. A Linux Workstation for High Performance Graphics

    NASA Technical Reports Server (NTRS)

    Geist, Robert; Westall, James

    2000-01-01

    The primary goal of this effort was to provide a low-cost method of obtaining high-performance 3-D graphics using an industry standard library (OpenGL) on PC class computers. Previously, users interested in doing substantial visualization or graphical manipulation were constrained to using specialized, custom hardware most often found in computers from Silicon Graphics (SGI). We provided an alternative to expensive SGI hardware by taking advantage of third-party, 3-D graphics accelerators that have now become available at very affordable prices. To make use of this hardware our goal was to provide a free, redistributable, and fully-compatible OpenGL work-alike library so that existing bodies of code could simply be recompiled. for PC class machines running a free version of Unix. This should allow substantial cost savings while greatly expanding the population of people with access to a serious graphics development and viewing environment. This should offer a means for NASA to provide a spectrum of graphics performance to its scientists, supplying high-end specialized SGI hardware for high-performance visualization while fulfilling the requirements of medium and lower performance applications with generic, off-the-shelf components and still maintaining compatibility between the two.

  2. High performance HRM: NHS employee perspectives.

    PubMed

    Hyde, Paula; Sparrow, Paul; Boaden, Ruth; Harris, Claire

    2013-01-01

    The purpose of this paper is to examine National Health Service (NHS) employee perspectives of how high performance human resource (HR) practices contribute to their performance. The paper draws on an extensive qualitative study of the NHS. A novel two-part method was used; the first part used focus group data from managers to identify high-performance HR practices specific to the NHS. Employees then conducted a card-sort exercise where they were asked how or whether the practices related to each other and how each practice affected their work. In total, 11 high performance HR practices relevant to the NHS were identified. Also identified were four reactions to a range of HR practices, which the authors developed into a typology according to anticipated beneficiaries (personal gain, organisation gain, both gain and no-one gains). Employees were able to form their own patterns (mental models) of performance contribution for a range of HR practices (60 interviewees produced 91 groupings). These groupings indicated three bundles particular to the NHS (professional development, employee contribution and NHS deal). These mental models indicate employee perceptions about how health services are organised and delivered in the NHS and illustrate the extant mental models of health care workers. As health services are rearranged and financial pressures begin to bite, these mental models will affect employee reactions to changes both positively and negatively. The novel method allows for identification of mental models that explain how NHS workers understand service delivery. It also delineates the complex and varied relationships between HR practices and individual performance.

  3. High Performance Commercial Fenestration Framing Systems

    SciTech Connect

    Mike Manteghi; Sneh Kumar; Joshua Early; Bhaskar Adusumalli

    2010-01-31

    A major objective of the U.S. Department of Energy is to have a zero energy commercial building by the year 2025. Windows have a major influence on the energy performance of the building envelope as they control over 55% of building energy load, and represent one important area where technologies can be developed to save energy. Aluminum framing systems are used in over 80% of commercial fenestration products (i.e. windows, curtain walls, store fronts, etc.). Aluminum framing systems are often required in commercial buildings because of their inherent good structural properties and long service life, which is required from commercial and architectural frames. At the same time, they are lightweight and durable, requiring very little maintenance, and offer design flexibility. An additional benefit of aluminum framing systems is their relatively low cost and easy manufacturability. Aluminum, being an easily recyclable material, also offers sustainable features. However, from energy efficiency point of view, aluminum frames have lower thermal performance due to the very high thermal conductivity of aluminum. Fenestration systems constructed of aluminum alloys therefore have lower performance in terms of being effective barrier to energy transfer (heat loss or gain). Despite the lower energy performance, aluminum is the choice material for commercial framing systems and dominates the commercial/architectural fenestration market because of the reasons mentioned above. In addition, there is no other cost effective and energy efficient replacement material available to take place of aluminum in the commercial/architectural market. Hence it is imperative to improve the performance of aluminum framing system to improve the energy performance of commercial fenestration system and in turn reduce the energy consumption of commercial building and achieve zero energy building by 2025. The objective of this project was to develop high performance, energy efficient commercial

  4. Population-based evaluation of the Ehrlichia ruminantium MAP 1B indirect ELISA.

    PubMed

    Peter, T F; O'Callaghan, C J; Medley, G F; Perry, B D; Semu, S M; Maha, S M

    2001-01-01

    The indirect MAP 1B ELISA based on the recombinant MAP 1B fragment of the immunodominant MAP I protein of Ehrlichia ruminantium is considered to be the most sensitive and specific assay for the serodiagnosis of heartwater. In this study, we evaluated its reliability in detecting exposure to E. ruminantium in field populations of domestic ruminants in Zimbabwe. Cattle and goat herds in endemically stable areas with high infection pressure and an expected close to 100% prevalence of E. ruminantium exposure were sampled. Bovine sera (858) and caprine sera (706) collected at seven locations representative of the two main production systems (communal lands and large scale commercial farms) in the two main agroecological zones of Zimbabwe (highveld and lowveld) were analysed. The prevalence of MAP 1B-specific antibodies in goats was similar and high, ranging from 67 to 100%, at all except one site (43%). Age-specific differences in goats (1, 2, 3, 4, 5 years) were not observed. In contrast, MAP 1B seroprevalence in cattle was significantly lower (P < 0.001), ranging from 46 to 61% in the lowveld communal area and from 24 to 33% in the remaining areas (P < 0.001). Age-specific differences in seroprevalence (1, 2, 3, 4, 5-7 + years) were similarly not evident in cattle (P < 0.15). Hence, the indirect MAP 1B ELISA may be an unreliable indicator of past exposure to heartwater in field-infected cattle in Zimbabwe. Although the reasons for this low response in field cattle are not fully understood, this study illustrates the need for field validation of the performance of new diagnostic tests prior to their use for epidemiological purposes.

  5. CYP1B1 expression, a potential risk factor for breast cancer

    SciTech Connect

    Goth-Goldstein, Regine; Erdmann, Christine A.; Russell, Marion

    2001-05-31

    CYP1B1 expression in non-tumor breast tissue from breast cancer patients and cancer-free individuals was determined to test the hypothesis that high CYP1B1 expression is a risk factor for breast cancer. Large interindividual variations in CYP1B1 expression were found with CYP1B1 levels notably higher in breast cancer patients than cancer-free individuals. The results indicate that CYP1B1 might play a role in breast cancer either through increased PAH activation or through metabolism of endogenous estrogen to a carcinogenic derivative.

  6. An Introduction to High Performance Computing

    NASA Astrophysics Data System (ADS)

    Almeida, Sérgio

    2013-09-01

    High Performance Computing (HPC) has become an essential tool in every researcher's arsenal. Most research problems nowadays can be simulated, clarified or experimentally tested by using computational simulations. Researchers struggle with computational problems when they should be focusing on their research problems. Since most researchers have little-to-no knowledge in low-level computer science, they tend to look at computer programs as extensions of their minds and bodies instead of completely autonomous systems. Since computers do not work the same way as humans, the result is usually Low Performance Computing where HPC would be expected.

  7. Inhibition of OATP1B1 by tyrosine kinase inhibitors: in vitro-in vivo correlations.

    PubMed

    Hu, S; Mathijssen, R H J; de Bruijn, P; Baker, S D; Sparreboom, A

    2014-02-18

    Several tyrosine kinase inhibitors (TKIs) can decrease docetaxel clearance in patients by an unknown mechanism. We hypothesised that these interactions are mediated by the hepatic uptake transporter OATP1B1. The influence of 16 approved TKIs on transport was studied in vitro using HEK293 cells expressing OATP1B1 or its mouse equivalent Oatp1b2. Pharmacokinetic studies were performed with Oatp1b2-knockout and OATP1B1-transgenic mice. All docetaxel-interacting TKIs, including sorafenib, were identified as potent inhibitors of OATP1B1 in vitro. Although Oatp1b2 deficiency in vivo was associated with increased docetaxel exposure, single- or multiple-dose sorafenib did not influence docetaxel pharmacokinetics. These findings highlight the importance of identifying proper preclinical models for verifying and predicting TKI-chemotherapy interactions involving transporters.

  8. Prediction of paclitaxel resistance in breast cancer: is CYP1B1*3 a new factor of influence?

    PubMed

    Gehrmann, Mathias; Schmidt, Markus; Brase, Jan C; Roos, Peter; Hengstler, Jan G

    2008-07-01

    This article focuses on the recent findings by Marsh and colleagues, and also discusses recent findings with regards to breast cancer. Taxanes are amongst the most active agents in the treatment of breast cancer. However, many tumors are intrinsically resistant. Therefore, it would be an enormous progress, if factors could be identified that reliably differentiate between taxane-sensitive and -resistant patients. Marsh and colleagues analyzed the CYP1B1*3 (Val432Leu) polymorphism in patients with high-risk stage III and IV breast cancer, who received dose-intense paclitaxel in combination with doxorubicin and cyclophosphamide. They report for the first time that patients with two leucine alleles in codon 432 of CYP1B1 experience a longer progression-free survival compared with patients with the Val/Leu or Val/Val genotypes. If confirmed in independent cohorts CYP1B1*3 may prove to be an important factor that helps to differentiate between paclitaxel-sensitive and resistant breast cancer patients. However, the mechanism behind the association between CYP1B1*3 and prognosis of paclitaxel-treated patients remains unclear. Several studies provide strong evidence that CYP1B1 does not influence tumor progression independently from paclitaxel chemotherapy, and that CYP1B1 itself does not alter paclitaxel resistance. In addition, CYP1B1 mRNA expression does not correlate with paclitaxel sensitivity of primary tumor cells. Although still speculative, a possible explanation is an association between CYP1B1*3 with still unknown factors that, on their part, influence paclitaxel sensitivity. In the future, studies with SNP chips and studies on the transcriptome, proteome and metabolome level should be performed in order to identify signatures differentiating between paclitaxel-sensitive and -resistant patients.

  9. Folding of active calcium channel beta(1b) -subunit by size-exclusion chromatography and its role on channel function.

    PubMed

    Neely, Alan; Garcia-Olivares, Jennie; Voswinkel, Stephan; Horstkott, Hannelore; Hidalgo, Patricia

    2004-05-21

    Voltage-gated calcium channels mediate the influx of Ca(2+) ions into eukaryotic cells in response to membrane depolarization. They are hetero-multimer membrane proteins formed by at least three subunits, the poreforming alpha(1)-subunit and the auxiliary beta- and alpha(2)delta-subunits. The beta-subunit is essential for channel performance because it regulates two distinct features of voltage-gated calcium channels, the surface expression and the channel activity. Four beta-subunit genes have been cloned, beta(1-4), with molecular masses ranging from 52 to 78 kDa, and several splice variants have been identified. The beta(1b)-subunit, expressed at high levels in mammalian brain, has been used extensively to study the interaction between the pore forming alpha(1)- and the regulatory beta-subunit. However, structural characterization has been impaired for its tendency to form aggregates when expressed in bacteria. We applied an on-column refolding procedure based on size exclusion chromatography to fold the beta(1b)-subunit of the voltage gated-calcium channels from Escherichia coli inclusion bodies. The beta(1b)-subunit refolds into monomers, as shown by sucrose gradient analysis, and binds to a glutathione S-transferase protein fused to the known target in the alpha(1)-subunit (the alpha-interaction domain). Using the cut-open oocyte voltage clamp technique, we measured gating and ionic currents in Xenopus oocytes expressing cardiac alpha(1)-subunit (alpha(1C)) co-injected with folded-beta(1b)-protein or beta(1b)-cRNA. We demonstrate that the co-expression of the alpha(1C)-subunit with either folded-beta(1b)-protein or beta(1b)-cRNA increases ionic currents to a similar extent and with no changes in charge movement, indicating that the beta(1b)-subunit primarily modulates channel activity, rather than expression.

  10. Multiple Intravenous Infusions Phase 1b

    PubMed Central

    Cassano-Piché, A; Fan, M; Sabovitch, S; Masino, C; Easty, AC

    2012-01-01

    Background Minimal research has been conducted into the potential patient safety issues related to administering multiple intravenous (IV) infusions to a single patient. Previous research has highlighted that there are a number of related safety risks. In Phase 1a of this study, an analysis of 2 national incident-reporting databases (Institute for Safe Medical Practices Canada and United States Food and Drug Administration MAUDE) found that a high percentage of incidents associated with the administration of multiple IV infusions resulted in patient harm. Objectives The primary objectives of Phase 1b of this study were to identify safety issues with the potential to cause patient harm stemming from the administration of multiple IV infusions; and to identify how nurses are being educated on key principles required to safely administer multiple IV infusions. Data Sources and Review Methods A field study was conducted at 12 hospital clinical units (sites) across Ontario, and telephone interviews were conducted with program coordinators or instructors from both the Ontario baccalaureate nursing degree programs and the Ontario postgraduate Critical Care Nursing Certificate programs. Data were analyzed using Rasmussen’s 1997 Risk Management Framework and a Health Care Failure Modes and Effects Analysis. Results Twenty-two primary patient safety issues were identified with the potential to directly cause patient harm. Seventeen of these (critical issues) were categorized into 6 themes. A cause-consequence tree was established to outline all possible contributing factors for each critical issue. Clinical recommendations were identified for immediate distribution to, and implementation by, Ontario hospitals. Future investigation efforts were planned for Phase 2 of the study. Limitations This exploratory field study identifies the potential for errors, but does not describe the direct observation of such errors, except in a few cases where errors were observed. Not all

  11. High performance FDTD algorithm for GPGPU supercomputers

    NASA Astrophysics Data System (ADS)

    Zakirov, Andrey; Levchenko, Vadim; Perepelkina, Anastasia; Zempo, Yasunari

    2016-10-01

    An implementation of FDTD method for solution of optical and other electrodynamic problems of high computational cost is described. The implementation is based on the LRnLA algorithm DiamondTorre, which is developed specifically for GPGPU hardware. The specifics of the DiamondTorre algorithms for staggered grid (Yee cell) and many-GPU devices are shown. The algorithm is implemented in the software for real physics calculation. The software performance is estimated through algorithms parameters and computer model. The real performance is tested on one GPU device, as well as on the many-GPU cluster. The performance of up to 0.65 • 1012 cell updates per second for 3D domain with 0.3 • 1012 Yee cells total is achieved.

  12. High performance forward swept wing aircraft

    NASA Technical Reports Server (NTRS)

    Koenig, David G. (Inventor); Aoyagi, Kiyoshi (Inventor); Dudley, Michael R. (Inventor); Schmidt, Susan B. (Inventor)

    1988-01-01

    A high performance aircraft capable of subsonic, transonic and supersonic speeds employs a forward swept wing planform and at least one first and second solution ejector located on the inboard section of the wing. A high degree of flow control on the inboard sections of the wing is achieved along with improved maneuverability and control of pitch, roll and yaw. Lift loss is delayed to higher angles of attack than in conventional aircraft. In one embodiment the ejectors may be advantageously positioned spanwise on the wing while the ductwork is kept to a minimum.

  13. Monitoring SLAC High Performance UNIX Computing Systems

    SciTech Connect

    Lettsome, Annette K.; /Bethune-Cookman Coll. /SLAC

    2005-12-15

    Knowledge of the effectiveness and efficiency of computers is important when working with high performance systems. The monitoring of such systems is advantageous in order to foresee possible misfortunes or system failures. Ganglia is a software system designed for high performance computing systems to retrieve specific monitoring information. An alternative storage facility for Ganglia's collected data is needed since its default storage system, the round-robin database (RRD), struggles with data integrity. The creation of a script-driven MySQL database solves this dilemma. This paper describes the process took in the creation and implementation of the MySQL database for use by Ganglia. Comparisons between data storage by both databases are made using gnuplot and Ganglia's real-time graphical user interface.

  14. Evaluation of high-performance computing software

    SciTech Connect

    Browne, S.; Dongarra, J.; Rowan, T.

    1996-12-31

    The absence of unbiased and up to date comparative evaluations of high-performance computing software complicates a user`s search for the appropriate software package. The National HPCC Software Exchange (NHSE) is attacking this problem using an approach that includes independent evaluations of software, incorporation of author and user feedback into the evaluations, and Web access to the evaluations. We are applying this approach to the Parallel Tools Library (PTLIB), a new software repository for parallel systems software and tools, and HPC-Netlib, a high performance branch of the Netlib mathematical software repository. Updating the evaluations with feed-back and making it available via the Web helps ensure accuracy and timeliness, and using independent reviewers produces unbiased comparative evaluations difficult to find elsewhere.

  15. Toward a theory of high performance.

    PubMed

    Kirby, Julia

    2005-01-01

    What does it mean to be a high-performance company? The process of measuring relative performance across industries and eras, declaring top performers, and finding the common drivers of their success is such a difficult one that it might seem a fool's errand to attempt. In fact, no one did for the first thousand or so years of business history. The question didn't even occur to many scholars until Tom Peters and Bob Waterman released In Search of Excellence in 1982. Twenty-three years later, we've witnessed several more attempts--and, just maybe, we're getting closer to answers. In this reported piece, HBR senior editor Julia Kirby explores why it's so difficult to study high performance and how various research efforts--including those from John Kotter and Jim Heskett; Jim Collins and Jerry Porras; Bill Joyce, Nitin Nohria, and Bruce Roberson; and several others outlined in a summary chart-have attacked the problem. The challenge starts with deciding which companies to study closely. Are the stars the ones with the highest market caps, the ones with the greatest sales growth, or simply the ones that remain standing at the end of the game? (And when's the end of the game?) Each major study differs in how it defines success, which companies it therefore declares to be worthy of emulation, and the patterns of activity and attitude it finds in common among them. Yet, Kirby concludes, as each study's method incrementally solves problems others have faced, we are progressing toward a consensus theory of high performance.

  16. High-performance reactionless scan mechanism

    NASA Technical Reports Server (NTRS)

    Williams, Ellen I.; Summers, Richard T.; Ostaszewski, Miroslaw A.

    1995-01-01

    A high-performance reactionless scan mirror mechanism was developed for space applications to provide thermal images of the Earth. The design incorporates a unique mechanical means of providing reactionless operation that also minimizes weight, mechanical resonance operation to minimize power, combined use of a single optical encoder to sense coarse and fine angular position, and a new kinematic mount of the mirror. A flex pivot hardware failure and current project status are discussed.

  17. High Performance Databases For Scientific Applications

    NASA Technical Reports Server (NTRS)

    French, James C.; Grimshaw, Andrew S.

    1997-01-01

    The goal for this task is to develop an Extensible File System (ELFS). ELFS attacks the problem of the following: 1. Providing high bandwidth performance architectures; 2. Reducing the cognitive burden faced by applications programmers when they attempt to optimize; and 3. Seamlessly managing the proliferation of data formats and architectural differences. The approach for ELFS solution consists of language and run-time system support that permits the specification on a hierarchy of file classes.

  18. AHPCRC - Army High Performance Computing Research Center

    DTIC Science & Technology

    2008-01-01

    materials “from the atoms up” or to model biological systems at the molecular level. The speed and capacity of massively parallel computers are key...Streamlined, massively parallel high performance computing structural codes allow researchers to examine many relevant physical factors simultaneously...expenditure of energy, so that the drones can carry their load of sensors, communications devices, and fuel. AHPCRC researchers are using massively

  19. High performance microsystem packaging: A perspective

    SciTech Connect

    Romig, A.D. Jr.; Dressendorfer, P.V.; Palmer, D.W.

    1997-10-01

    The second silicon revolution will be based on intelligent, integrated microsystems where multiple technologies (such as analog, digital, memory, sensor, micro-electro-mechanical, and communication devices) are integrated onto a single chip or within a multichip module. A necessary element for such systems is cost-effective, high-performance packaging. This paper examines many of the issues associated with the packaging of integrated microsystems, with an emphasis on the areas of packaging design, manufacturability, and reliability.

  20. Tough, High-Performance, Thermoplastic Addition Polymers

    NASA Technical Reports Server (NTRS)

    Pater, Ruth H.; Proctor, K. Mason; Gleason, John; Morgan, Cassandra; Partos, Richard

    1991-01-01

    Series of addition-type thermoplastics (ATT's) exhibit useful properties. Because of their addition curing and linear structure, ATT polymers have toughness, like thermoplastics, and easily processed, like thermosets. Work undertaken to develop chemical reaction forming stable aromatic rings in backbone of ATT polymer, combining high-temperature performance and thermo-oxidative stability with toughness and easy processibility, and minimizing or eliminating necessity for tradeoffs among properties often observed in conventional polymer syntheses.

  1. Noise of High Performance Aircraft at Afterburner

    DTIC Science & Technology

    2016-05-04

    Research Project Title: Noise of High-Performance Aircraft at Afterburner Principal Investigator Dr. Christopher Tam Department of...During this quarter, our research effort concentrated on processing and understanding the real time noise data of the F-18E aircraft . There...18E aircraft tones are combustor resonances. At this time, in the absence of other independent data, we are unable to conclude whether the tones of

  2. Development of high performance BWR spacer

    SciTech Connect

    Morooka, Shinichi; Shirakawa, Kenetu; Mitutake, Tohru; Yamamoto, Yasushi; Yano, Takashi; Kimura, Jiro

    1996-07-01

    The spacer has a significant effect on thermal hydraulic performance of BWR fuel assembly. The purpose of this study is to develop a new BWR spacer with high critical power and low pressure drop performance. The developed high performance spacer is a ferrule type spacer with twisted tape and improved flow tab. This spacer is called CYCLONE spacer. Critical power and pressure drop have been measured at BEST (BWR Experimental Loop for Stability and Transient test) of Toshiba Corporation. The test bundle consists of electrically heated rods in a 4x4 array configuration. These heater rods are indirectly heated. The heated length and outer diameter of the heater rod, as well as the number and the axial locations of the spacers, are the same as for those for a BWR fuel assembly. The axial power shape is stepped cosine (1.4 of the maximum peaking factor). Two test assemblies with different radial power distribution have been used. One test assembly has the maximum power rods at the center of the test assembly and the other has the maximum power rods near the channel wall. The results show that the critical power performance of CYCLONE spacer is 10 to 25 % higher than that of the ferrule spacers, while the pressure drop for CYCLONE spacer is nearly equal to that of the ferrule spacer.

  3. High temperature furnace modeling and performance verifications

    NASA Technical Reports Server (NTRS)

    Smith, James E., Jr.

    1992-01-01

    Analytical, numerical, and experimental studies were performed on two classes of high temperature materials processing sources for their potential use as directional solidification furnaces. The research concentrated on a commercially available high temperature furnace using a zirconia ceramic tube as the heating element and an Arc Furnace based on a tube welder. The first objective was to assemble the zirconia furnace and construct parts needed to successfully perform experiments. The 2nd objective was to evaluate the zirconia furnace performance as a directional solidification furnace element. The 3rd objective was to establish a data base on materials used in the furnace construction, with particular emphasis on emissivities, transmissivities, and absorptivities as functions of wavelength and temperature. A 1-D and 2-D spectral radiation heat transfer model was developed for comparison with standard modeling techniques, and were used to predict wall and crucible temperatures. The 4th objective addressed the development of a SINDA model for the Arc Furnace and was used to design sample holders and to estimate cooling media temperatures for the steady state operation of the furnace. And, the 5th objective addressed the initial performance evaluation of the Arc Furnace and associated equipment for directional solidification. Results of these objectives are presented.

  4. Computational Biology and High Performance Computing 2000

    SciTech Connect

    Simon, Horst D.; Zorn, Manfred D.; Spengler, Sylvia J.; Shoichet, Brian K.; Stewart, Craig; Dubchak, Inna L.; Arkin, Adam P.

    2000-10-19

    The pace of extraordinary advances in molecular biology has accelerated in the past decade due in large part to discoveries coming from genome projects on human and model organisms. The advances in the genome project so far, happening well ahead of schedule and under budget, have exceeded any dreams by its protagonists, let alone formal expectations. Biologists expect the next phase of the genome project to be even more startling in terms of dramatic breakthroughs in our understanding of human biology, the biology of health and of disease. Only today can biologists begin to envision the necessary experimental, computational and theoretical steps necessary to exploit genome sequence information for its medical impact, its contribution to biotechnology and economic competitiveness, and its ultimate contribution to environmental quality. High performance computing has become one of the critical enabling technologies, which will help to translate this vision of future advances in biology into reality. Biologists are increasingly becoming aware of the potential of high performance computing. The goal of this tutorial is to introduce the exciting new developments in computational biology and genomics to the high performance computing community.

  5. Elephant endotheliotropic herpesviruses EEHV1A, EEHV1B, and EEHV2 from cases of hemorrhagic disease are highly diverged from other mammalian herpesviruses and may form a new subfamily.

    PubMed

    Richman, Laura K; Zong, Jian-Chao; Latimer, Erin M; Lock, Justin; Fleischer, Robert C; Heaggans, Sarah Y; Hayward, Gary S

    2014-12-01

    A family of novel endotheliotropic herpesviruses (EEHVs) assigned to the genus Proboscivirus have been identified as the cause of fatal hemorrhagic disease in 70 young Asian elephants worldwide. Although EEHV cannot be grown in cell culture, we have determined a total of 378 kb of viral genomic DNA sequence directly from clinical tissue samples from six lethal cases and two survivors. Overall, the data obtained encompass 57 genes, including orthologues of 32 core genes common to all herpesviruses, 14 genes found in some other herpesviruses, plus 10 novel genes, including a single large putative transcriptional regulatory protein (ORF-L). On the basis of differences in gene content and organization plus phylogenetic analyses of conserved core proteins that have just 20% to 50% or less identity to orthologues in other herpesviruses, we propose that EEHV1A, EEHV1B, and EEHV2 could be considered a new Deltaherpesvirinae subfamily of mammalian herpesviruses that evolved as an intermediate branch between the Betaherpesvirinae and Gammaherpesvirinae. Unlike cytomegaloviruses, EEHV genomes encode ribonucleotide kinase B subunit (RRB), thymidine kinase (TK), and UL9-like origin binding protein (OBP) proteins and have an alphaherpesvirus-like dyad symmetry Ori-Lyt domain. They also differ from all known betaherpesviruses by having a 40-kb large-scale inversion of core gene blocks I, II, and III. EEHV1 and EEHV2 DNA differ uniformly by more than 25%, but EEHV1 clusters into two major subgroups designated EEHV1A and EEHV1B with ancient partially chimeric features. Whereas large segments are nearly identical, three nonadjacent loci totaling 15 kb diverge by between 21 and 37%. One strain of EEHV1B analyzed is interpreted to be a modern partial recombinant with EEHV1A. Asian elephants are an endangered species whose survival is under extreme pressure in wild range countries and whose captive breeding populations in zoos are not self-sustaining. In 1999, a novel class of

  6. Elephant Endotheliotropic Herpesviruses EEHV1A, EEHV1B, and EEHV2 from Cases of Hemorrhagic Disease Are Highly Diverged from Other Mammalian Herpesviruses and May Form a New Subfamily

    PubMed Central

    Richman, Laura K.; Zong, Jian-Chao; Latimer, Erin M.; Lock, Justin; Fleischer, Robert C.; Heaggans, Sarah Y.

    2014-01-01

    ABSTRACT A family of novel endotheliotropic herpesviruses (EEHVs) assigned to the genus Proboscivirus have been identified as the cause of fatal hemorrhagic disease in 70 young Asian elephants worldwide. Although EEHV cannot be grown in cell culture, we have determined a total of 378 kb of viral genomic DNA sequence directly from clinical tissue samples from six lethal cases and two survivors. Overall, the data obtained encompass 57 genes, including orthologues of 32 core genes common to all herpesviruses, 14 genes found in some other herpesviruses, plus 10 novel genes, including a single large putative transcriptional regulatory protein (ORF-L). On the basis of differences in gene content and organization plus phylogenetic analyses of conserved core proteins that have just 20% to 50% or less identity to orthologues in other herpesviruses, we propose that EEHV1A, EEHV1B, and EEHV2 could be considered a new Deltaherpesvirinae subfamily of mammalian herpesviruses that evolved as an intermediate branch between the Betaherpesvirinae and Gammaherpesvirinae. Unlike cytomegaloviruses, EEHV genomes encode ribonucleotide kinase B subunit (RRB), thymidine kinase (TK), and UL9-like origin binding protein (OBP) proteins and have an alphaherpesvirus-like dyad symmetry Ori-Lyt domain. They also differ from all known betaherpesviruses by having a 40-kb large-scale inversion of core gene blocks I, II, and III. EEHV1 and EEHV2 DNA differ uniformly by more than 25%, but EEHV1 clusters into two major subgroups designated EEHV1A and EEHV1B with ancient partially chimeric features. Whereas large segments are nearly identical, three nonadjacent loci totaling 15 kb diverge by between 21 and 37%. One strain of EEHV1B analyzed is interpreted to be a modern partial recombinant with EEHV1A. IMPORTANCE Asian elephants are an endangered species whose survival is under extreme pressure in wild range countries and whose captive breeding populations in zoos are not self-sustaining. In 1999, a

  7. Towards High-Assurance High-Performance Program Synthesis

    NASA Technical Reports Server (NTRS)

    Lowry, Michael; Roach, Steven; vanBaalen, Jeffrey

    1997-01-01

    Domain-specific automatic program synthesis tools, also called application generators, are playing an ever-increasing role in software development. However, high-performance application generators require difficult manual construction, and are very difficult to verify correct. This paper describes research and an implemented system that transforms program synthesis tools based on deductive synthesis into high-performance application generators. Deductive synthesis uses theorem-proving to construct solutions when given problem specifications. The verification condition for a deductive synthesis tool is essentially the soundness of the implemented inference rules. Theory Operationalization for Program Synthesis (TOPS) synergistically combines reformulation, automated mathematical classification, and compilation through partial deduction to decision procedures. It transforms general-purpose deductive synthesis, with exponential performance, into efficient special-purpose deductive synthesis, with near-linear performance. This paper describes our experience with and empirical results of PD(TH) theory-based partial deduction - in which partial deduction of a set of first-order formulae is performed within the context of a background theory. The implemented TOPS system currently performs a special variant of PD(TH) in which the compilation process results in the transformation of a set of first order formulae into the theory of an instantiated library decision procedure augmented by a compiled unit theory.

  8. N-Glycosylation of Serum IgG and Total Glycoproteins in MAN1B1 Deficiency.

    PubMed

    Saldova, Radka; Stöckmann, Henning; O'Flaherty, Roisin; Lefeber, Dirk J; Jaeken, Jaak; Rudd, Pauline M

    2015-10-02

    MAN1B1-CDG has recently been characterized as a type II congenital disorder of glycosylation (CDG), disrupting not only protein N-glycosylation but also general Golgi morphology. Using our high-throughput, quantitative ultra-performance liquid chromatography assay, we achieved a detailed characterization of the glycosylation changes in both total serum glycoproteins and isolated serum IgG from ten previously reported MAN1B1-CDG patients. We have identified and quantified novel hybrid high-mannosylated MAN1B1-CDG-specific IgG glycans and found an increase of sialyl Lewis x (sLex) glycans on serum proteins of all patients. This increase in sLex has not been previously reported in any CDG. These findings may provide insight into the pathophysiology of this CDG.

  9. High Efficiency CRISPR/Cas9-mediated Gene Editing in Primary Human T-cells Using Mutant Adenoviral E4orf6/E1b55k "Helper" Proteins.

    PubMed

    Gwiazda, Kamila S; Grier, Alexandra E; Sahni, Jaya; Burleigh, Stephen M; Martin, Unja; Yang, Julia G; Popp, Nicholas A; Krutein, Michelle C; Khan, Iram F; Jacoby, Kyle; Jensen, Michael C; Rawlings, David J; Scharenberg, Andrew M

    2016-09-29

    Many future therapeutic applications of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 and related RNA-guided nucleases are likely to require their use to promote gene targeting, thus necessitating development of methods that provide for delivery of three components-Cas9, guide RNAs and recombination templates-to primary cells rendered proficient for homology-directed repair. Here, we demonstrate an electroporation/transduction codelivery method that utilizes mRNA to express both Cas9 and mutant adenoviral E4orf6 and E1b55k helper proteins in association with adeno-associated virus (AAV) vectors expressing guide RNAs and recombination templates. By transiently enhancing target cell permissiveness to AAV transduction and gene editing efficiency, this novel approach promotes efficient gene disruption and/or gene targeting at multiple loci in primary human T-cells, illustrating its broad potential for application in translational gene editing.

  10. Lipidomics reveals a link between CYP1B1 and SCD1 in promoting obesity.

    PubMed

    Li, Fei; Jiang, Changtao; Larsen, Michele C; Bushkofsky, Justin; Krausz, Kristopher W; Wang, Ting; Jefcoate, Colin R; Gonzalez, Frank J

    2014-05-02

    Cytochrome P450 1B1 (CYP1B1) is involved in the metabolism of xenobiotic compounds and endogenous metabolites. Disruption of Cyp1b1 in mice results in suppression of high-fat diet (HFD)-induced obesity and an extensive change in hepatic energy regulation despite minimal constitutive expression of CYP1B1 in hepatocytes. Lack of CYP1B1 is correlated with altered lipid metabolism, especially lysophosphatidylcholines, contributing to protection against obesity. Ultraperformance liquid chromatography coupled to electrospray ionization quadrupole mass spectrometry (UPLC-ESI-QTOFMS)-based metabolomics revealed lysophosphatidylcholine 18:0 (LPC 18:0) as a biomarker positively related to HFD-induced obesity. The increased serum LPC 18:0 in wild-type mice is reduced in Cyp1b1-null mice on a HFD, which is reversed in CYP1B1-humanized mice. CYP1B1-humanized mice show higher diet-induced obesity compared with Cyp1b1-null mice, suggesting that human CYP1B1 shows a similar response to HFD as mouse Cyp1b1. In addition, hepatic stearoyl-CoA desaturase 1 (SCD1) expression was decreased in Cyp1b1-null mice, and the attenuated diet-induced obesity and lower serum LPC 18:0 in the Cyp1b1-null mice is elevated after SCD1 overexpression, suggesting that SCD1 is correlated with CYP1B1-induced obesity. These studies establish a biochemical link between cytochromes P450, lipids, and metabolic disorders and suggest that inhibition of CYP1B1 could be target for antiobesity drugs.

  11. High Performance High-Tc Superconducting Wires

    SciTech Connect

    Kang, Sukill; Goyal, Amit; Li, Jing; Gapud, Albert Agcaoili; Martin, Patrick M; Heatherly Jr, Lee; Thompson, James R; Christen, David K; List III, Frederick Alyious; Paranthaman, Mariappan Parans; Lee, Dominic F

    2006-01-01

    We demonstrated short segments of a superconducting wire that meets or exceeds performance requirements for many large-scale applications of high-temperature superconducting materials, especially those requiring a high supercurrent and/or a high engineering critical current density in applied magnetic fields. The performance requirements for these varied applications were met in 3-micrometer-thick YBa{sub 2}Cu{sub 3}O{sub 7-{delta}} films epitaxially grown via pulsed laser ablation on rolling assisted biaxially textured substrates. Enhancements of the critical current in self-field as well as excellent retention of this current in high applied magnetic fields were achieved in the thick films via incorporation of a periodic array of extended columnar defects, composed of self-aligned nanodots of nonsuperconducting material extending through the entire thickness of the film. These columnar defects are highly effective in pinning the superconducting vortices or flux lines, thereby resulting in the substantially enhanced performance of this wire.

  12. Oleanane triterpenes as protein tyrosine phosphatase 1B (PTP1B) inhibitors from Camellia japonica.

    PubMed

    Uddin, Mohammad Nasir; Sharma, Govinda; Yang, Jun-Li; Choi, Hong Seok; Lim, Seong-Il; Kang, Keon Wook; Oh, Won Keun

    2014-07-01

    Protein tyrosine phosphatase 1B (PTP1B) plays a key role in metabolic signaling, thereby making it an exciting drug target for type 2 diabetes and obesity. Besides, there is substantial evidence that shows its overexpression is involved in breast cancer, which suggests that selective PTP1B inhibition might be effective in breast cancer treatment. As part of our continuous research on PTP1B inhibitors from medicinal plants, four oleanane-type triterpenes were isolated from an EtOAc-soluble extract of fruit peels of Camellia japonica (Theaceae), together with 6 previously known compounds of this class. Their structures were determined on the basis of spectroscopic data analysis (UV, IR, (1)H and (13)CNMR, HMBC, HSQC, NOESY, and MS). All isolates were evaluated for their inhibitory effects on PTP1B, as well as their cytotoxic effects against human breast cancer cell lines MCF7, MCF7/ADR, and MDA-MB-231. Several compounds with OH-3 or/and COOH-28 functionalities showed strong PTP1B inhibitory activity (IC50 values ranging from 3.77±0.11 to 6.40±0.81 μM) as well as significant cytotoxicity (IC50 values ranging from 0.51±0.05 to 13.55±1.44 μM).

  13. The path toward HEP High Performance Computing

    NASA Astrophysics Data System (ADS)

    Apostolakis, John; Brun, René; Carminati, Federico; Gheata, Andrei; Wenzel, Sandro

    2014-06-01

    High Energy Physics code has been known for making poor use of high performance computing architectures. Efforts in optimising HEP code on vector and RISC architectures have yield limited results and recent studies have shown that, on modern architectures, it achieves a performance between 10% and 50% of the peak one. Although several successful attempts have been made to port selected codes on GPUs, no major HEP code suite has a "High Performance" implementation. With LHC undergoing a major upgrade and a number of challenging experiments on the drawing board, HEP cannot any longer neglect the less-than-optimal performance of its code and it has to try making the best usage of the hardware. This activity is one of the foci of the SFT group at CERN, which hosts, among others, the Root and Geant4 project. The activity of the experiments is shared and coordinated via a Concurrency Forum, where the experience in optimising HEP code is presented and discussed. Another activity is the Geant-V project, centred on the development of a highperformance prototype for particle transport. Achieving a good concurrency level on the emerging parallel architectures without a complete redesign of the framework can only be done by parallelizing at event level, or with a much larger effort at track level. Apart the shareable data structures, this typically implies a multiplication factor in terms of memory consumption compared to the single threaded version, together with sub-optimal handling of event processing tails. Besides this, the low level instruction pipelining of modern processors cannot be used efficiently to speedup the program. We have implemented a framework that allows scheduling vectors of particles to an arbitrary number of computing resources in a fine grain parallel approach. The talk will review the current optimisation activities within the SFT group with a particular emphasis on the development perspectives towards a simulation framework able to profit best from

  14. Heavily Doped PBSE with High Thermoelectric Performance

    NASA Technical Reports Server (NTRS)

    Snyder, G. Jeffrey (Inventor); Wang, Heng (Inventor); Pei, Yanzhong (Inventor)

    2015-01-01

    The present invention discloses heavily doped PbSe with high thermoelectric performance. Thermoelectric property measurements disclosed herein indicated that PbSe is high zT material for mid-to-high temperature thermoelectric applications. At 850 K a peak zT (is) greater than 1.3 was observed when n(sub H) approximately 1.0 X 10(exp 20) cm(exp -3). The present invention also discloses that a number of strategies used to improve zT of PbTe, such as alloying with other elements, nanostructuring and band modification may also be used to further improve zT in PbSe.

  15. High Performance Fortran for Aerospace Applications

    NASA Technical Reports Server (NTRS)

    Mehrotra, Piyush; Zima, Hans; Bushnell, Dennis M. (Technical Monitor)

    2000-01-01

    This paper focuses on the use of High Performance Fortran (HPF) for important classes of algorithms employed in aerospace applications. HPF is a set of Fortran extensions designed to provide users with a high-level interface for programming data parallel scientific applications, while delegating to the compiler/runtime system the task of generating explicitly parallel message-passing programs. We begin by providing a short overview of the HPF language. This is followed by a detailed discussion of the efficient use of HPF for applications involving multiple structured grids such as multiblock and adaptive mesh refinement (AMR) codes as well as unstructured grid codes. We focus on the data structures and computational structures used in these codes and on the high-level strategies that can be expressed in HPF to optimally exploit the parallelism in these algorithms.

  16. High Performance Database Management for Earth Sciences

    NASA Technical Reports Server (NTRS)

    Rishe, Naphtali; Barton, David; Urban, Frank; Chekmasov, Maxim; Martinez, Maria; Alvarez, Elms; Gutierrez, Martha; Pardo, Philippe

    1998-01-01

    The High Performance Database Research Center at Florida International University is completing the development of a highly parallel database system based on the semantic/object-oriented approach. This system provides exceptional usability and flexibility. It allows shorter application design and programming cycles and gives the user control via an intuitive information structure. It empowers the end-user to pose complex ad hoc decision support queries. Superior efficiency is provided through a high level of optimization, which is transparent to the user. Manifold reduction in storage size is allowed for many applications. This system allows for operability via internet browsers. The system will be used for the NASA Applications Center program to store remote sensing data, as well as for Earth Science applications.

  17. Interleukin 1B Variant -1473G/C (rs1143623) Influences Triglyceride and Interleukin 6 Metabolism

    PubMed Central

    Delgado-Lista, Javier; Garcia-Rios, Antonio; Perez-Martinez, Pablo; Solivera, Juan; Yubero-Serrano, Elena M.; Fuentes, Francisco; Parnell, Laurence D.; Shen, Jian; Gomez, Purificacion; Jimenez-Gomez, Yolanda; Gomez-Luna, Maria J.; Marin, Carmen; Belisle, Sarah E.; Rodriguez-Cantalejo, Fernando; Meydani, Simin N.; Ordovas, Jose M.; Perez-Jimenez, Francisco

    2011-01-01

    Context: IL1b (IL1B or IL1β), a key modulator of the immune response, exerts its functions mainly via IL6 regulation. Fatty meals cause transient hypertriglyceridemia and are considered to be proinflammatory, but the extent of these responses shows high interindividual susceptibility. Objective: We evaluated the influence of a genetic variant located in the promoter region of IL1B (-1473G/C) on fasting and postprandial lipids and IL6. Design, Setting, and Participants: A total of 477 people over age 65 yr were genotyped for IL1B -1473G/C, and we evaluated fasting lipids depending on genotype. Then, 88 healthy young men were also genotyped and were fed a saturated fatty acid-rich meal. Serial blood samples were drawn for 11 h after the meal, and lipid fractions and IL6 were assayed. Main Outcome and Interventions: Fasting lipids were studied in the aged persons. Fasting and postprandial measurements of lipids and IL6 were performed in the healthy young men. Results: In the aged persons, CC subjects (minor allele homozygotes) showed higher triglyceride (P = 0.002) and cholesterol (P = 0.011) levels. Healthy young male carriers of the minor C allele showed higher postprandial triglycerides (P = 0.037), and those carried into large triglyceride-rich lipoproteins (P = 0.004). In addition, they showed higher postprandial IL6 concentrations (P = 0.008). Conclusions: Our work shows that inflammatory genes may regulate fasting and postprandial lipids because the carriers of the minor allele of an IL gene variant have altered lipid metabolism. To reinforce these gene-phenotype findings, IL6 (the natural effector of IL1B) was increased in these persons. PMID:21307135

  18. Interleukin 1B variant -1473G/C (rs1143623) influences triglyceride and interleukin 6 metabolism.

    PubMed

    Delgado-Lista, Javier; Garcia-Rios, Antonio; Perez-Martinez, Pablo; Solivera, Juan; Yubero-Serrano, Elena M; Fuentes, Francisco; Parnell, Laurence D; Shen, Jian; Gomez, Purificacion; Jimenez-Gomez, Yolanda; Gomez-Luna, Maria J; Marin, Carmen; Belisle, Sarah E; Rodriguez-Cantalejo, Fernando; Meydani, Simin N; Ordovas, Jose M; Perez-Jimenez, Francisco; Lopez-Miranda, Jose

    2011-05-01

    IL1b (IL1B or IL1β), a key modulator of the immune response, exerts its functions mainly via IL6 regulation. Fatty meals cause transient hypertriglyceridemia and are considered to be proinflammatory, but the extent of these responses shows high interindividual susceptibility. We evaluated the influence of a genetic variant located in the promoter region of IL1B (-1473G/C) on fasting and postprandial lipids and IL6. A total of 477 people over age 65 yr were genotyped for IL1B -1473G/C, and we evaluated fasting lipids depending on genotype. Then, 88 healthy young men were also genotyped and were fed a saturated fatty acid-rich meal. Serial blood samples were drawn for 11 h after the meal, and lipid fractions and IL6 were assayed. MAIN OUTCOME AND INTERVENTIONS: Fasting lipids were studied in the aged persons. Fasting and postprandial measurements of lipids and IL6 were performed in the healthy young men. In the aged persons, CC subjects (minor allele homozygotes) showed higher triglyceride (P = 0.002) and cholesterol (P = 0.011) levels. Healthy young male carriers of the minor C allele showed higher postprandial triglycerides (P = 0.037), and those carried into large triglyceride-rich lipoproteins (P = 0.004). In addition, they showed higher postprandial IL6 concentrations (P = 0.008). Our work shows that inflammatory genes may regulate fasting and postprandial lipids because the carriers of the minor allele of an IL gene variant have altered lipid metabolism. To reinforce these gene-phenotype findings, IL6 (the natural effector of IL1B) was increased in these persons.

  19. High capacity heat pipe performance demonstration

    NASA Technical Reports Server (NTRS)

    1983-01-01

    A high capacity heat pipe which will operate in one-g and in zero-g is investigated. An artery configuration which is self-priming in one-g was emphasized. Two artery modifications were evolved as candidates to achieve one-g priming and will provide the very high performance: the four artery and the eight artery configurations. These were each evaluated analytically for performance and priming capability. The eight artery configuration was found to be inadequate from a performance standpoint. The four artery showed promise of working. A five-inch long priming element test article was fabricated using the four artery design. Plexiglas viewing windows were made on each end of the heat pipe to permit viewing of the priming activity. The five-inch primary element would not successfully prime in one-g. Difficulties on priming in one-g raised questions about zero-g priming. Therefore a small test element heat pipe for verifying that the proposed configuration will self-prime in zero-g was fabricated and delivered.

  20. A High Performance COTS Based Computer Architecture

    NASA Astrophysics Data System (ADS)

    Patte, Mathieu; Grimoldi, Raoul; Trautner, Roland

    2014-08-01

    Using Commercial Off The Shelf (COTS) electronic components for space applications is a long standing idea. Indeed the difference in processing performance and energy efficiency between radiation hardened components and COTS components is so important that COTS components are very attractive for use in mass and power constrained systems. However using COTS components in space is not straightforward as one must account with the effects of the space environment on the COTS components behavior. In the frame of the ESA funded activity called High Performance COTS Based Computer, Airbus Defense and Space and its subcontractor OHB CGS have developed and prototyped a versatile COTS based architecture for high performance processing. The rest of the paper is organized as follows: in a first section we will start by recapitulating the interests and constraints of using COTS components for space applications; then we will briefly describe existing fault mitigation architectures and present our solution for fault mitigation based on a component called the SmartIO; in the last part of the paper we will describe the prototyping activities executed during the HiP CBC project.

  1. Management issues for high performance storage systems

    SciTech Connect

    Louis, S.; Burris, R.

    1995-03-01

    Managing distributed high-performance storage systems is complex and, although sharing common ground with traditional network and systems management, presents unique storage-related issues. Integration technologies and frameworks exist to help manage distributed network and system environments. Industry-driven consortia provide open forums where vendors and users cooperate to leverage solutions. But these new approaches to open management fall short addressing the needs of scalable, distributed storage. We discuss the motivation and requirements for storage system management (SSM) capabilities and describe how SSM manages distributed servers and storage resource objects in the High Performance Storage System (HPSS), a new storage facility for data-intensive applications and large-scale computing. Modem storage systems, such as HPSS, require many SSM capabilities, including server and resource configuration control, performance monitoring, quality of service, flexible policies, file migration, file repacking, accounting, and quotas. We present results of initial HPSS SSM development including design decisions and implementation trade-offs. We conclude with plans for follow-on work and provide storage-related recommendations for vendors and standards groups seeking enterprise-wide management solutions.

  2. High-resolution SAR ATR performance analysis

    NASA Astrophysics Data System (ADS)

    Douglas, Joel; Burke, Monica; Ettinger, Gil J.

    2004-09-01

    High resolution Synthetic Aperture Radar (SAR) imagery (e.g., four inch or better resolution) contains features not seen in one foot or lower resolution imagery, due to the isolation of the scatterers into separate resolution cells. These features provide the potential for additional discrimination power for Automatic Target Recognition (ATR) systems. In this paper, we analyze the performance of the Real-Time MSTAR (RT-MSTAR) system as a function of image resolution. Performance is measured both in terms of the probability of correct identification on military targets, and also in terms of confuser rejection. The analysis demonstrates two factors that significantly enhance performance. First, use of the high resolution imagery results in much higher probability of correct identification, as demonstrated using Lynx SAR imagery at 4" and 12". Second, incorporating models of the confusers, when available, greatly reduces false alarms, even at higher resolutions. Several new areas of work emerge, including making use of higher-level feature information available in the imagery, and rapid creation of models for vehicles that pose particular confuser rejection challenges.

  3. Automatic Energy Schemes for High Performance Applications

    SciTech Connect

    Sundriyal, Vaibhav

    2013-01-01

    Although high-performance computing traditionally focuses on the efficient execution of large-scale applications, both energy and power have become critical concerns when approaching exascale. Drastic increases in the power consumption of supercomputers affect significantly their operating costs and failure rates. In modern microprocessor architectures, equipped with dynamic voltage and frequency scaling (DVFS) and CPU clock modulation (throttling), the power consumption may be controlled in software. Additionally, network interconnect, such as Infiniband, may be exploited to maximize energy savings while the application performance loss and frequency switching overheads must be carefully balanced. This work first studies two important collective communication operations, all-to-all and allgather and proposes energy saving strategies on the per-call basis. Next, it targets point-to-point communications to group them into phases and apply frequency scaling to them to save energy by exploiting the architectural and communication stalls. Finally, it proposes an automatic runtime system which combines both collective and point-to-point communications into phases, and applies throttling to them apart from DVFS to maximize energy savings. The experimental results are presented for NAS parallel benchmark problems as well as for the realistic parallel electronic structure calculations performed by the widely used quantum chemistry package GAMESS. Close to the maximum energy savings were obtained with a substantially low performance loss on the given platform.

  4. RISC Processors and High Performance Computing

    NASA Technical Reports Server (NTRS)

    Saini, Subhash; Bailey, David H.; Lasinski, T. A. (Technical Monitor)

    1995-01-01

    In this tutorial, we will discuss top five current RISC microprocessors: The IBM Power2, which is used in the IBM RS6000/590 workstation and in the IBM SP2 parallel supercomputer, the DEC Alpha, which is in the DEC Alpha workstation and in the Cray T3D; the MIPS R8000, which is used in the SGI Power Challenge; the HP PA-RISC 7100, which is used in the HP 700 series workstations and in the Convex Exemplar; and the Cray proprietary processor, which is used in the new Cray J916. The architecture of these microprocessors will first be presented. The effective performance of these processors will then be compared, both by citing standard benchmarks and also in the context of implementing a real applications. In the process, different programming models such as data parallel (CM Fortran and HPF) and message passing (PVM and MPI) will be introduced and compared. The latest NAS Parallel Benchmark (NPB) absolute performance and performance per dollar figures will be presented. The next generation of the NP13 will also be described. The tutorial will conclude with a discussion of general trends in the field of high performance computing, including likely future developments in hardware and software technology, and the relative roles of vector supercomputers tightly coupled parallel computers, and clusters of workstations. This tutorial will provide a unique cross-machine comparison not available elsewhere.

  5. Towards high performance inverted polymer solar cells

    NASA Astrophysics Data System (ADS)

    Gong, Xiong

    2013-03-01

    Bulk heterojunction polymer solar cells that can be fabricated by solution processing techniques are under intense investigation in both academic institutions and industrial companies because of their potential to enable mass production of flexible and cost-effective alternative to silicon-based electronics. Despite the envisioned advantages and recent technology advances, so far the performance of polymer solar cells is still inferior to inorganic counterparts in terms of the efficiency and stability. There are many factors limiting the performance of polymer solar cells. Among them, the optical and electronic properties of materials in the active layer, device architecture and elimination of PEDOT:PSS are the most determining factors in the overall performance of polymer solar cells. In this presentation, I will present how we approach high performance of polymer solar cells. For example, by developing novel materials, fabrication polymer photovoltaic cells with an inverted device structure and elimination of PEDOT:PSS, we were able to observe over 8.4% power conversion efficiency from inverted polymer solar cells.

  6. Performance of the CMS High Level Trigger

    NASA Astrophysics Data System (ADS)

    Perrotta, Andrea

    2015-12-01

    The CMS experiment has been designed with a 2-level trigger system. The first level is implemented using custom-designed electronics. The second level is the so-called High Level Trigger (HLT), a streamlined version of the CMS offline reconstruction software running on a computer farm. For Run II of the Large Hadron Collider, the increases in center-of-mass energy and luminosity will raise the event rate to a level challenging for the HLT algorithms. The increase in the number of interactions per bunch crossing, on average 25 in 2012, and expected to be around 40 in Run II, will be an additional complication. We present here the expected performance of the main triggers that will be used during the 2015 data taking campaign, paying particular attention to the new approaches that have been developed to cope with the challenges of the new run. This includes improvements in HLT electron and photon reconstruction as well as better performing muon triggers. We will also present the performance of the improved tracking and vertexing algorithms, discussing their impact on the b-tagging performance as well as on the jet and missing energy reconstruction.

  7. High Power MPD Thruster Performance Measurements

    NASA Technical Reports Server (NTRS)

    LaPointe, Michael R.; Strzempkowski, Eugene; Pencil, Eric

    2004-01-01

    High power magnetoplasmadynamic (MPD) thrusters are being developed as cost effective propulsion systems for cargo transport to lunar and Mars bases, crewed missions to Mars and the outer planets, and robotic deep space exploration missions. Electromagnetic MPD thrusters have demonstrated, at the laboratory level, the ability to process megawatts of electrical power while providing significantly higher thrust densities than electrostatic electric propulsion systems. The ability to generate higher thrust densities permits a reduction in the number of thrusters required to perform a given mission, and alleviates the system complexity associated with multiple thruster arrays. The specific impulse of an MPD thruster can be optimized to meet given mission requirements, from a few thousand seconds with heavier gas propellants up to 10,000 seconds with hydrogen propellant. In support of programs envisioned by the NASA Office of Exploration Systems, Glenn Research Center is developing and testing quasi-steady MW-class MPD thrusters as a prelude to steady state high power thruster tests. This paper provides an overview of the GRC high power pulsed thruster test facility, and presents preliminary performance data for a quasi-steady baseline MPD thruster geometry.

  8. DOE High Performance Concentrator PV Project

    SciTech Connect

    McConnell, R.; Symko-Davies, M.

    2005-08-01

    Much in demand are next-generation photovoltaic (PV) technologies that can be used economically to make a large-scale impact on world electricity production. The U.S. Department of Energy (DOE) initiated the High-Performance Photovoltaic (HiPerf PV) Project to substantially increase the viability of PV for cost-competitive applications so that PV can contribute significantly to both our energy supply and environment. To accomplish such results, the National Center for Photovoltaics (NCPV) directs in-house and subcontracted research in high-performance polycrystalline thin-film and multijunction concentrator devices with the goal of enabling progress of high-efficiency technologies toward commercial-prototype products. We will describe the details of the subcontractor and in-house progress in exploring and accelerating pathways of III-V multijunction concentrator solar cells and systems toward their long-term goals. By 2020, we anticipate that this project will have demonstrated 33% system efficiency and a system price of $1.00/Wp for concentrator PV systems using III-V multijunction solar cells with efficiencies over 41%.

  9. High-performance computing in seismology

    SciTech Connect

    1996-09-01

    The scientific, technical, and economic importance of the issues discussed here presents a clear agenda for future research in computational seismology. In this way these problems will drive advances in high-performance computing in the field of seismology. There is a broad community that will benefit from this work, including the petroleum industry, research geophysicists, engineers concerned with seismic hazard mitigation, and governments charged with enforcing a comprehensive test ban treaty. These advances may also lead to new applications for seismological research. The recent application of high-resolution seismic imaging of the shallow subsurface for the environmental remediation industry is an example of this activity. This report makes the following recommendations: (1) focused efforts to develop validated documented software for seismological computations should be supported, with special emphasis on scalable algorithms for parallel processors; (2) the education of seismologists in high-performance computing technologies and methodologies should be improved; (3) collaborations between seismologists and computational scientists and engineers should be increased; (4) the infrastructure for archiving, disseminating, and processing large volumes of seismological data should be improved.

  10. A high performance architecture for prolog

    SciTech Connect

    Dobry, T.

    1987-01-01

    Artificial Intelligence is entering the mainstream of computer applications and as techniques are developed and integrated into a wide variety of areas they are beginning to tax the processing power of conventional architecture. To meet this demand, specialized architectures providing support for the unique features of symbolic processing languages are emerging. The goal of the research presented here is to show that an architecture specialized for Prolog can achieve a ten-fold improvement in performance over conventional general-purpose architecture, and presents such an architecture for high performance execution of Prolog programs. The architecture is based on the abstract machine description known as the Warren Abstract Machine (WAM). The execution model of the WAM is described and extended to provide a complete Instruction Set Architecture (ISA) for Prolog known as the PLM. The ISA is then realized in a microarchitecture and finally in a hardware design.

  11. High-performance architecture for Prolog

    SciTech Connect

    Dobry, T.P.

    1987-01-01

    Artificial intelligence is entering the mainstream of computer applications and, as techniques are developed and integrated into a wide variety of areas, they are beginning to tax the processing power of conventional architectures. To meet this demand, specialized architectures providing support for the unique features of symbolic processing languages are emerging. The goal of the research presented here is to show that an architecture specialized for Prolog can achieve a tenfold improvement in performance over conventional, general-purpose architectures. This dissertation presents such an architecture for high performance execution of Prolog programs. The architecture is based on the abstract machine description introduced by David H.D. Warren known as the Warren Abstract Machine (WAM). The execution model of the WAM is described and extended to provide a complete Instruction Set Architecture (ISA) for Prolog known as the PLM. This ISA is then realized in a microarchitecture and finally in a hardware design.

  12. Proven high-performance display solution

    NASA Astrophysics Data System (ADS)

    Johnson, Rick J.; Shaw, James E.; Mosier, Don; Liss, Raymond L.; Prouty, Todd D.; Davis, Josh; Marzen, Vincent P.; Deloy, Christian T.

    2002-08-01

    Rockwell Collins serves both the military and the commercial segments by exploiting the common elements of these applications. Rockwell Collins has created a liquid crystal display family capable of 100:1 contrast ratio, 40:1 high ambient contrast, 0.25% specular reflectance, 0.1% diffuse reflectance, enhanced color stability over +/- 55H, 0-30V field of view, 300 fL with 10K:1 dimming range, color NVIS B compliance while exceeding environmental performance requirements though ruggedization. In order to meet the full range of display requirements at a system level, all the components must be understood and managed to meet the end solution of the final system. This paper details Rockwell Collins' optical performance using an avionics grade panel, third generation custom compensation, and solid state backlight.

  13. The high performance solar array GSR3

    NASA Astrophysics Data System (ADS)

    Mamode, A.; Bartevian, J.; Bastard, J. L.; Auffray, P.; Plagne, A.

    A foldout solar array for communication satellites was developed. A wing composed of 4 panels of 1.6 x 1.5 m and a Y-shaped yoke, and a wing with 3 panels of 2.4 x 2.4 m were made. End of life performance goal is greater than 35 W/kg with BSR 180 micron solar cells, and 50 W/kg using 50 micron BSFR cells. Analysis shows that all identified requirements can be covered with current skin made of open weave very high modulus carbon fiber; reinforcements of unidirectional carbon fiber; honeycomb in current section; hold-down inserts made of wound carbon fibers; titanium hinge fitting; and Kapton foil (25 or 50 micron thickness). Tests confirm performance predictions.

  14. Flexible high-performance IR camera systems

    NASA Astrophysics Data System (ADS)

    Hoelter, Theodore R.; Petronio, Susan M.; Carralejo, Ronald J.; Frank, Jeffery D.; Graff, John H.

    1999-07-01

    Indigo Systems Corporation has developed a family of standard readout integrated circuits (ROIC) for use in IR focal plane arrays (FPAs) imaging systems. These standard ROICs are designed to provide a compete set of operating features for camera level FPA control, while also providing high performance capability with any of several detector materials. By creating a uniform electrical interface for FPAs, these standard ROICs simplify the task of FPA integration with imaging electronics and physical packages. This paper begins with a brief description of the features of four Indigo standard ROICs and continues with a description of the features, design, and measured performance of indium antimonide, quantum well IR photo- detectors and indium gallium arsenide imaging system built using the described standard ROICs.

  15. High performance robotic traverse of desert terrain.

    SciTech Connect

    Whittaker, William

    2004-09-01

    This report presents tentative innovations to enable unmanned vehicle guidance for a class of off-road traverse at sustained speeds greater than 30 miles per hour. Analyses and field trials suggest that even greater navigation speeds might be achieved. The performance calls for innovation in mapping, perception, planning and inertial-referenced stabilization of components, hosted aboard capable locomotion. The innovations are motivated by the challenge of autonomous ground vehicle traverse of 250 miles of desert terrain in less than 10 hours, averaging 30 miles per hour. GPS coverage is assumed to be available with localized blackouts. Terrain and vegetation are assumed to be akin to that of the Mojave Desert. This terrain is interlaced with networks of unimproved roads and trails, which are a key to achieving the high performance mapping, planning and navigation that is presented here.

  16. TMF ultra-high rate discharge performance

    SciTech Connect

    Nelson, B.

    1997-12-01

    BOLDER Technologies Corporation has developed a valve-regulated lead-acid product line termed Thin Metal Film (TMF{trademark}) technology. It is characterized by extremely thin plates and close plate spacing that facilitate high rates of charge and discharge with minimal temperature increases, at levels unachievable with other commercially-available battery technologies. This ultra-high rate performance makes TMF technology ideal for such applications as various types of engine start, high drain rate portable devices and high-current pulsing. Data are presented on very high current continuous and pulse discharges. Power and energy relationships at various discharge rates are explored and the fast-response characteristics of the BOLDER{reg_sign} cell are qualitatively defined. Short-duration recharge experiments will show that devices powered by BOLDER batteries can be in operation for more than 90% of an extended usage period with multiple fast recharges. The BOLDER cell is ideal for applications such as engine-start, a wide range of portable devices including power tools, hybrid electric vehicles and pulse-power devices. Applications such as this are very attractive, and are well served by TMF technology, but an area of great interest and excitement is ultrahigh power delivery in excess of 1 kW/kg.

  17. Improving UV Resistance of High Performance Fibers

    NASA Astrophysics Data System (ADS)

    Hassanin, Ahmed

    High performance fibers are characterized by their superior properties compared to the traditional textile fibers. High strength fibers have high modules, high strength to weight ratio, high chemical resistance, and usually high temperature resistance. It is used in application where superior properties are needed such as bulletproof vests, ropes and cables, cut resistant products, load tendons for giant scientific balloons, fishing rods, tennis racket strings, parachute cords, adhesives and sealants, protective apparel and tire cords. Unfortunately, Ultraviolet (UV) radiation causes serious degradation to the most of high performance fibers. UV lights, either natural or artificial, cause organic compounds to decompose and degrade, because the energy of the photons of UV light is high enough to break chemical bonds causing chain scission. This work is aiming at achieving maximum protection of high performance fibers using sheathing approaches. The sheaths proposed are of lightweight to maintain the advantage of the high performance fiber that is the high strength to weight ratio. This study involves developing three different types of sheathing. The product of interest that need be protected from UV is braid from PBO. First approach is extruding a sheath from Low Density Polyethylene (LDPE) loaded with different rutile TiO2 % nanoparticles around the braid from the PBO. The results of this approach showed that LDPE sheath loaded with 10% TiO2 by weight achieved the highest protection compare to 0% and 5% TiO2. The protection here is judged by strength loss of PBO. This trend noticed in different weathering environments, where the sheathed samples were exposed to UV-VIS radiations in different weatheromter equipments as well as exposure to high altitude environment using NASA BRDL balloon. The second approach is focusing in developing a protective porous membrane from polyurethane loaded with rutile TiO2 nanoparticles. Membrane from polyurethane loaded with 4

  18. CYP1B1 Polymorphism as a Risk Factor for Race-Related Prostate Cancer

    DTIC Science & Technology

    2008-06-01

    AD_________________ Award Number: W81XWH-04-1-0579 TITLE: CYP1B1 Polymorphism as a Risk Factor...TITLE AND SUBTITLE CYP1B1 Polymorphism as a Risk Factor for Race-Related Prostate Cancer 5a. CONTRACT NUMBER 5b. GRANT NUMBER W81XWH-04-1-0579...collected; as well as further experimentation with aim #1to be performed. 15. SUBJECT TERMS CYP1B1 , Polymorphism , Prostate cancer, Race-related

  19. High performance channel injection sealant invention abstract

    NASA Technical Reports Server (NTRS)

    Rosser, R. W.; Basiulis, D. I.; Salisbury, D. P. (Inventor)

    1982-01-01

    High performance channel sealant is based on NASA patented cyano and diamidoximine-terminated perfluoroalkylene ether prepolymers that are thermally condensed and cross linked. The sealant contains asbestos and, in its preferred embodiments, Lithofrax, to lower its thermal expansion coefficient and a phenolic metal deactivator. Extensive evaluation shows the sealant is extremely resistant to thermal degradation with an onset point of 280 C. The materials have a volatile content of 0.18%, excellent flexibility, and adherence properties, and fuel resistance. No corrosibility to aluminum or titanium was observed.

  20. High-Performance Water-Iodinating Cartridge

    NASA Technical Reports Server (NTRS)

    Sauer, Richard; Gibbons, Randall E.; Flanagan, David T.

    1993-01-01

    High-performance cartridge contains bed of crystalline iodine iodinates water to near saturation in single pass. Cartridge includes stainless-steel housing equipped with inlet and outlet for water. Bed of iodine crystals divided into layers by polytetrafluoroethylene baffles. Holes made in baffles and positioned to maximize length of flow path through layers of iodine crystals. Resulting concentration of iodine biocidal; suppresses growth of microbes in stored water or disinfects contaminated equipment. Cartridge resists corrosion and can be stored wet. Reused several times before necessary to refill with fresh iodine crystals.

  1. High performance thyratron driver with low jitter.

    PubMed

    Verma, Rishi; Lee, P; Springham, S V; Tan, T L; Rawat, R S

    2007-08-01

    We report the design and development of insulated gate bipolar junction transistor based high performance driver for operating thyratrons in grounded grid mode. With careful design, the driver meets the specification of trigger output pulse rise time less than 30 ns, jitter less than +/-1 ns, and time delay less than 160 ns. It produces a -600 V pulse of 500 ns duration (full width at half maximum) at repetition rate ranging from 1 Hz to 1.14 kHz. The developed module also facilitates heating and biasing units along with protection circuitry in one complete package.

  2. High performance thyratron driver with low jitter

    NASA Astrophysics Data System (ADS)

    Verma, Rishi; Lee, P.; Springham, S. V.; Tan, T. L.; Rawat, R. S.

    2007-08-01

    We report the design and development of insulated gate bipolar junction transistor based high performance driver for operating thyratrons in grounded grid mode. With careful design, the driver meets the specification of trigger output pulse rise time less than 30ns, jitter less than ±1ns, and time delay less than 160ns. It produces a -600V pulse of 500ns duration (full width at half maximum) at repetition rate ranging from 1Hzto1.14kHz. The developed module also facilitates heating and biasing units along with protection circuitry in one complete package.

  3. High-performance neural networks. [Neural computers

    SciTech Connect

    Dress, W.B.

    1987-06-01

    The new Forth hardware architectures offer an intermediate solution to high-performance neural networks while the theory and programming details of neural networks for synthetic intelligence are developed. This approach has been used successfully to determine the parameters and run the resulting network for a synthetic insect consisting of a 200-node ''brain'' with 1760 interconnections. Both the insect's environment and its sensor input have thus far been simulated. However, the frequency-coded nature of the Browning network allows easy replacement of the simulated sensors by real-world counterparts.

  4. BEDOPS: high-performance genomic feature operations.

    PubMed

    Neph, Shane; Kuehn, M Scott; Reynolds, Alex P; Haugen, Eric; Thurman, Robert E; Johnson, Audra K; Rynes, Eric; Maurano, Matthew T; Vierstra, Jeff; Thomas, Sean; Sandstrom, Richard; Humbert, Richard; Stamatoyannopoulos, John A

    2012-07-15

    The large and growing number of genome-wide datasets highlights the need for high-performance feature analysis and data comparison methods, in addition to efficient data storage and retrieval techniques. We introduce BEDOPS, a software suite for common genomic analysis tasks which offers improved flexibility, scalability and execution time characteristics over previously published packages. The suite includes a utility to compress large inputs into a lossless format that can provide greater space savings and faster data extractions than alternatives. http://code.google.com/p/bedops/ includes binaries, source and documentation.

  5. High-Performance Water-Iodinating Cartridge

    NASA Technical Reports Server (NTRS)

    Sauer, Richard; Gibbons, Randall E.; Flanagan, David T.

    1993-01-01

    High-performance cartridge contains bed of crystalline iodine iodinates water to near saturation in single pass. Cartridge includes stainless-steel housing equipped with inlet and outlet for water. Bed of iodine crystals divided into layers by polytetrafluoroethylene baffles. Holes made in baffles and positioned to maximize length of flow path through layers of iodine crystals. Resulting concentration of iodine biocidal; suppresses growth of microbes in stored water or disinfects contaminated equipment. Cartridge resists corrosion and can be stored wet. Reused several times before necessary to refill with fresh iodine crystals.

  6. High Performance Polymer Memory and Its Formation

    DTIC Science & Technology

    2007-04-26

    Std. Z39.18 Final Report to AFOSR High Performance Polymer Memory Device and Its Formation Fund No.: FA9550-04-1-0215 Prepared by Prof. Yang Yang...polystyrene (PS). The metal nanoparticles were prepared by the two-phase 10-5 (b) 10𔄁Polymer film 1a CC , 10, Glass 1 -2 -1 0 1 2 3 4 5 Bias (V) Fig. I...such as copper pthalocyanine (CuPc), 24 ൢ zinc pthalocyanine (ZnPc), 27󈧠 tetracene, 29 and pentacene 30 have been used as donors combined with

  7. High Performance Piezoelectric Actuated Gimbal (HIERAX)

    SciTech Connect

    Charles Tschaggeny; Warren Jones; Eberhard Bamberg

    2007-04-01

    This paper presents a 3-axis gimbal whose three rotational axes are actuated by a novel drive system: linear piezoelectric motors whose linear output is converted to rotation by using drive disks. Advantages of this technology are: fast response, high accelerations, dither-free actuation and backlash-free positioning. The gimbal was developed to house a laser range finder for the purpose of tracking and guiding unmanned aerial vehicles during landing maneuvers. The tilt axis was built and the test results indicate excellent performance that meets design specifications.

  8. High Performance Computing for complex fluids simulation

    NASA Astrophysics Data System (ADS)

    Ismail, Mourad; Chabannes, Vincent; Doyeux, Vincent; Priem, Stéphane; Prud'Homme, Christophe; Feelpp Team

    2014-11-01

    In order to better understand the behavior of complex fluids in general and blood flow in particular, several models have been proposed by considering blood as a Newtonian fluid (governed by the Stokes equations or Navier-Stokes) in which are immersed deformable entities. These particles contain a second fluid of different viscosity and density from outer fluid. This context, I will present some models based on the same principle and will show its validations using some known benchmarks. I will also talk briefly about High Performance Computing in the framework of complex fluids simulations. http://www.feelpp.org/

  9. High-temperature testing of high performance fiber reinforced concrete

    NASA Astrophysics Data System (ADS)

    Fořt, Jan; Vejmelková, Eva; Pavlíková, Milena; Trník, Anton; Čítek, David; Kolísko, Jiří; Černý, Robert; Pavlík, Zbyšek

    2016-06-01

    The effect of high-temperature exposure on properties of High Performance Fiber Reinforced Concrete (HPFRC) is researched in the paper. At first, reference measurements are done on HPFRC samples without high-temperature loading. Then, the HPFRC samples are exposed to the temperatures of 200, 400, 600, 800, and 1000 °C. For the temperature loaded samples, measurement of residual mechanical and basic physical properties is done. Linear thermal expansion coefficient as function of temperature is accessed on the basis of measured thermal strain data. Additionally, simultaneous difference scanning calorimetry (DSC) and thermogravimetry (TG) analysis is performed in order to observe and explain material changes at elevated temperature. It is found that the applied high temperature loading significantly increases material porosity due to the physical, chemical and combined damage of material inner structure, and negatively affects also the mechanical strength. Linear thermal expansion coefficient exhibits significant dependence on temperature and changes of material structure. The obtained data will find use as input material parameters for modelling the damage of HPFRC structures exposed to the fire and high temperature action.

  10. High field transport of high performance black phosphorus transistors

    NASA Astrophysics Data System (ADS)

    Li, Tiaoyang; Zhang, Zhenfeng; Li, Xuefei; Huang, Mingqiang; Li, Sichao; Li, Shengman; Wu, Yanqing

    2017-04-01

    Black phosphorus is a layered material stacked together by weak van der Waals force with a direct bandgap and highly anisotropic electrical characteristics. Most of the previous reports focus on the low-field mobility of transistors based on SiO2 back gate dielectrics. Recently, black phosphorus transistors encapsulated with hexagonal boron nitride have been demonstrated with greatly improved mobility at low temperatures. However, this approach requires multiple dry transfer methods using both black phosphorus and boron nitride flakes, which are only available in small crystal sizes. Here, we demonstrated high performance black phosphorus transistors using atomic layer deposited high-κ HfO2 as a back gate dielectric. The maximum drain current density reaches 480 μA/μm at 300 K and a record high drain current 906 μA/μm at 20 K in a short channel 100 nm device based on HfO2, exhibiting excellent current-carrying capability and high field strength. Moreover, a side-by-side comparison on important figures-of-merit is carried out systematically for transistors based on HfO2 with those based on conventional SiO2, showing more than 50% performance improvement in mobility and over 8 times reduction in interface trap density.

  11. Performance Evaluation of Emerging High Performance Computing Technologies using WRF

    NASA Astrophysics Data System (ADS)

    Newby, G. B.; Morton, D.

    2008-12-01

    The Arctic Region Supercomputing Center (ARSC) has evaluated multicore processors and other emerging processor technologies for a variety of high performance computing applications in the earth and space sciences, especially climate and weather applications. A flagship effort has been to assess dual core processor nodes on ARSC's Midnight supercomputer, in which two-socket systems were compared to eight-socket systems. Midnight is utilized for ARSC's twice-daily weather research and forecasting (WRF) model runs, available at weather.arsc.edu. Among other findings on Midnight, it was found that the Hypertransport system for interconnecting Opteron processors, memory, and other subsystems does not scale as well on eight-socket (sixteen processor) systems as well as two-socket (four processor) systems. A fundamental limitation is the cache snooping operation performed whenever a computational thread accesses main memory. This increases memory latency as the number of processor sockets increases. This is particularly noticeable on applications such as WRF that are primarily CPU-bound, versus applications that are bound by input/output or communication. The new Cray XT5 supercomputer at ARSC features quad core processors, and will host a variety of scaling experiments for WRF, CCSM4, and other models. Early results will be presented, including a series of WRF runs for Alaska with grid resolutions under 2km. ARSC will discuss a set of standardized test cases for the Alaska domain, similar to existing test cases for CONUS. These test cases will provide different configuration sizes and resolutions, suitable for single processors up to thousands. Beyond multi-core Opteron-based supercomputers, ARSC has examined WRF and other applications on additional emerging technologies. One such technology is the graphics processing unit, or GPU. The 9800-series nVidia GPU was evaluated with the cuBLAS software library. While in-socket GPUs might be forthcoming in the future, current

  12. High temperature furnace modeling and performance verifications

    NASA Technical Reports Server (NTRS)

    Smith, James E., Jr.

    1988-01-01

    Analytical, numerical and experimental studies were performed on two classes of high temperature materials processing furnaces. The research concentrates on a commercially available high temperature furnace using zirconia as the heating element and an arc furnace based on a ST International tube welder. The zirconia furnace was delivered and work is progressing on schedule. The work on the arc furnace was initially stalled due to the unavailability of the NASA prototype, which is actively being tested aboard the KC-135 experimental aircraft. A proposal was written and funded to purchase an additional arc welder to alleviate this problem. The ST International weld head and power supply were received and testing will begin in early November. The first 6 months of the grant are covered.

  13. Parallel Algebraic Multigrid Methods - High Performance Preconditioners

    SciTech Connect

    Yang, U M

    2004-11-11

    The development of high performance, massively parallel computers and the increasing demands of computationally challenging applications have necessitated the development of scalable solvers and preconditioners. One of the most effective ways to achieve scalability is the use of multigrid or multilevel techniques. Algebraic multigrid (AMG) is a very efficient algorithm for solving large problems on unstructured grids. While much of it can be parallelized in a straightforward way, some components of the classical algorithm, particularly the coarsening process and some of the most efficient smoothers, are highly sequential, and require new parallel approaches. This chapter presents the basic principles of AMG and gives an overview of various parallel implementations of AMG, including descriptions of parallel coarsening schemes and smoothers, some numerical results as well as references to existing software packages.

  14. High performances imaging systems for planetary landers

    NASA Astrophysics Data System (ADS)

    Josset, J.-L.; Beauvivre, S.

    2003-04-01

    Each planetary mission brings its specific needs and environmental conditions: high temperature and radiations for Mercury, shock, thermal cycles and low temperature operation for Mars, long vacuum cruise phase and very low temperature for comet nucleus. Nevertheless, all the missions share the same interests in term of low mass, low power and harsh environmental conditions. When a mission includes a lander, mass optimization is even more critical for the benefit of the overall science return. SPACE-X has developed high-performances imaging systems for Rosetta Lander and MarsExpress Lander. Future imaging systems for new exploration missions have to consider the promising micro-nano-technology developments in terms of miniaturisation, low power, wireless capabilities, etc.

  15. High Performance Data Distribution for Scientific Community

    NASA Astrophysics Data System (ADS)

    Tirado, Juan M.; Higuero, Daniel; Carretero, Jesus

    2010-05-01

    Institutions such as NASA, ESA or JAXA find solutions to distribute data from their missions to the scientific community, and their long term archives. This is a complex problem, as it includes a vast amount of data, several geographically distributed archives, heterogeneous architectures with heterogeneous networks, and users spread around the world. We propose a novel architecture (HIDDRA) that solves this problem aiming to reduce user intervention in data acquisition and processing. HIDDRA is a modular system that provides a highly efficient parallel multiprotocol download engine, using a publish/subscribe policy which helps the final user to obtain data of interest transparently. Our system can deal simultaneously with multiple protocols (HTTP,HTTPS, FTP, GridFTP among others) to obtain the maximum bandwidth, reducing the workload in data server and increasing flexibility. It can also provide high reliability and fault tolerance, as several sources of data can be used to perform one file download. HIDDRA architecture can be arranged into a data distribution network deployed on several sites that can cooperate to provide former features. HIDDRA has been addressed by the 2009 e-IRG Report on Data Management as a promising initiative for data interoperability. Our first prototype has been evaluated in collaboration with the ESAC centre in Villafranca del Castillo (Spain) that shows a high scalability and performance, opening a wide spectrum of opportunities. Some preliminary results have been published in the Journal of Astrophysics and Space Science [1]. [1] D. Higuero, J.M. Tirado, J. Carretero, F. Félix, and A. de La Fuente. HIDDRA: a highly independent data distribution and retrieval architecture for space observation missions. Astrophysics and Space Science, 321(3):169-175, 2009

  16. Lithostratigraphy from downhole logs in Hole AND-1B, Antarctica

    USGS Publications Warehouse

    Williams, Trevor; Morin, Roger H.; Jarrard, Richard D.; Jackolski, Chris L.; Henrys, Stuart A.; Niessen, Frank; Magens, Diana; Kuhn, Gerhard; Monien, Donata; Powell, Ross D.

    2012-01-01

    The ANDRILL (Antarctic Drilling Project) McMurdo Ice Shelf (MIS) project drilled 1285 m of sediment in Hole AND–1B, representing the past 12 m.y. of glacial history. Downhole geophysical logs were acquired to a depth of 1018 mbsf (meters below seafloor), and are complementary to data acquired from the core. The natural gamma radiation (NGR) and magnetic susceptibility logs are particularly useful for understanding lithological and paleoenvironmental change at ANDRILL McMurdo Ice Shelf Hole AND–1B. NGR logs cover the entire interval from the seafloor to 1018 mbsf, and magnetic susceptibility and other logs covered the open hole intervals between 692 and 1018 and 237–342 mbsf. In the upper part of AND–1B, clear alternations between low and high NGR values distinguish between diatomite (lacking minerals containing naturally radioactive K, U, and Th) and diamictite (containing K-bearing clays, K-feldspar, mica, and heavy minerals). In the lower open hole logged section, NGR and magnetic susceptibility can also distinguish claystones (rich in K-bearing clay minerals, relatively low in magnetite) and diamictites (relatively high in magnetite). Sandstones can be distinguished by their high resistivity values in AND–1B. On the basis of these three downhole logs, diamictite, claystones, and sandstones can be predicted correctly for 74% of the 692–1018 mbsf interval. The logs were then used to predict facies for the 6% of this interval that was unrecovered by coring. Given the understanding of the physical property characteristics of different facies, it is also possible to identify subtle changes in lithology from the physical properties and help refine parts of the lithostratigraphy, for example, the varying terrigenous content of diatomites and the transitions from subice diamictite to open-water diatomite.

  17. High-performance laboratories and cleanrooms

    SciTech Connect

    Tschudi, William; Sartor, Dale; Mills, Evan; Xu, Tengfang

    2002-07-01

    The California Energy Commission sponsored this roadmap to guide energy efficiency research and deployment for high performance cleanrooms and laboratories. Industries and institutions utilizing these building types (termed high-tech buildings) have played an important part in the vitality of the California economy. This roadmap's key objective to present a multi-year agenda to prioritize and coordinate research efforts. It also addresses delivery mechanisms to get the research products into the market. Because of the importance to the California economy, it is appropriate and important for California to take the lead in assessing the energy efficiency research needs, opportunities, and priorities for this market. In addition to the importance to California's economy, energy demand for this market segment is large and growing (estimated at 9400 GWH for 1996, Mills et al. 1996). With their 24hr. continuous operation, high tech facilities are a major contributor to the peak electrical demand. Laboratories and cleanrooms constitute the high tech building market, and although each building type has its unique features, they are similar in that they are extremely energy intensive, involve special environmental considerations, have very high ventilation requirements, and are subject to regulations--primarily safety driven--that tend to have adverse energy implications. High-tech buildings have largely been overlooked in past energy efficiency research. Many industries and institutions utilize laboratories and cleanrooms. As illustrated, there are many industries operating cleanrooms in California. These include semiconductor manufacturing, semiconductor suppliers, pharmaceutical, biotechnology, disk drive manufacturing, flat panel displays, automotive, aerospace, food, hospitals, medical devices, universities, and federal research facilities.

  18. Energy Efficient Graphene Based High Performance Capacitors.

    PubMed

    Bae, Joonwon; Lee, Chang-Soo; Kwon, Oh Seok

    2016-10-27

    Graphene (GRP) is an interesting class of nano-structured electronic materials for various cutting-edge applications. To date, extensive research activities have been performed on the investigation of diverse properties of GRP. The incorporation of this elegant material can be very lucrative in terms of practical applications in energy storage/conversion systems. Among various those systems, high performance electrochemical capacitors (ECs) have become popular due to the recent need for energy efficient and portable devices. Therefore, in this article, the application of GRP for capacitors is described succinctly. In particular, a concise summary on the previous research activities regarding GRP based capacitors is also covered extensively. It was revealed that a lot of secondary materials such as polymers and metal oxides have been introduced to improve the performance. Also, diverse devices have been combined with capacitors for better use. More importantly, recent patents related to the preparation and application of GRP based capacitors are also introduced briefly. This article can provide essential information for future study.

  19. High-performance computing for airborne applications

    SciTech Connect

    Quinn, Heather M; Manuzzato, Andrea; Fairbanks, Tom; Dallmann, Nicholas; Desgeorges, Rose

    2010-06-28

    Recently, there has been attempts to move common satellite tasks to unmanned aerial vehicles (UAVs). UAVs are significantly cheaper to buy than satellites and easier to deploy on an as-needed basis. The more benign radiation environment also allows for an aggressive adoption of state-of-the-art commercial computational devices, which increases the amount of data that can be collected. There are a number of commercial computing devices currently available that are well-suited to high-performance computing. These devices range from specialized computational devices, such as field-programmable gate arrays (FPGAs) and digital signal processors (DSPs), to traditional computing platforms, such as microprocessors. Even though the radiation environment is relatively benign, these devices could be susceptible to single-event effects. In this paper, we will present radiation data for high-performance computing devices in a accelerated neutron environment. These devices include a multi-core digital signal processor, two field-programmable gate arrays, and a microprocessor. From these results, we found that all of these devices are suitable for many airplane environments without reliability problems.

  20. Climate Modeling using High-Performance Computing

    SciTech Connect

    Mirin, A A; Wickett, M E; Duffy, P B; Rotman, D A

    2005-03-03

    The Center for Applied Scientific Computing (CASC) and the LLNL Atmospheric Science Division (ASD) are working together to improve predictions of future climate by applying the best available computational methods and computer resources to this problem. Over the last decade, researchers at the Lawrence Livermore National Laboratory (LLNL) have developed a number of climate models that provide state-of-the-art simulations on a wide variety of massively parallel computers. We are now developing and applying a second generation of high-performance climate models. As part of LLNL's participation in DOE's Scientific Discovery through Advanced Computing (SciDAC) program, members of CASC and ASD are collaborating with other DOE labs and NCAR in the development of a comprehensive, next-generation global climate model. This model incorporates the most current physics and numerics and capably exploits the latest massively parallel computers. One of LLNL's roles in this collaboration is the scalable parallelization of NASA's finite-volume atmospheric dynamical core. We have implemented multiple two-dimensional domain decompositions, where the different decompositions are connected by high-speed transposes. Additional performance is obtained through shared memory parallelization constructs and one-sided interprocess communication. The finite-volume dynamical core is particularly important to atmospheric chemistry simulations, where LLNL has a leading role.

  1. High Performance Computing CFRD -- Final Technial Report

    SciTech Connect

    Hope Forsmann; Kurt Hamman

    2003-01-01

    The Bechtel Waste Treatment Project (WTP), located in Richland, WA, is comprised of many processes containing complex physics. Accurate analyses of the underlying physics of these processes is needed to reduce the amount of added costs during and after construction that are due to unknown process behavior. The WTP will have tight operating margins in order to complete the treatment of the waste on schedule. The combination of tight operating constraints coupled with complex physical processes requires analysis methods that are more accurate than traditional approaches. This study is focused specifically on multidimensional computer aided solutions. There are many skills and tools required to solve engineering problems. Many physical processes are governed by nonlinear partial differential equations. These governing equations have few, if any, closed form solutions. Past and present solution methods require assumptions to reduce these equations to solvable forms. Computational methods take the governing equations and solve them directly on a computational grid. This ability to approach the equations in their exact form reduces the number of assumptions that must be made. This approach increases the accuracy of the solution and its applicability to the problem at hand. Recent advances in computer technology have allowed computer simulations to become an essential tool for problem solving. In order to perform computer simulations as quickly and accurately as possible, both hardware and software must be evaluated. With regards to hardware, the average consumer personal computers (PCs) are not configured for optimal scientific use. Only a few vendors create high performance computers to satisfy engineering needs. Software must be optimized for quick and accurate execution. Operating systems must utilize the hardware efficiently while supplying the software with seamless access to the computer’s resources. From the perspective of Bechtel Corporation and the Idaho

  2. SISYPHUS: A high performance seismic inversion factory

    NASA Astrophysics Data System (ADS)

    Gokhberg, Alexey; Simutė, Saulė; Boehm, Christian; Fichtner, Andreas

    2016-04-01

    In the recent years the massively parallel high performance computers became the standard instruments for solving the forward and inverse problems in seismology. The respective software packages dedicated to forward and inverse waveform modelling specially designed for such computers (SPECFEM3D, SES3D) became mature and widely available. These packages achieve significant computational performance and provide researchers with an opportunity to solve problems of bigger size at higher resolution within a shorter time. However, a typical seismic inversion process contains various activities that are beyond the common solver functionality. They include management of information on seismic events and stations, 3D models, observed and synthetic seismograms, pre-processing of the observed signals, computation of misfits and adjoint sources, minimization of misfits, and process workflow management. These activities are time consuming, seldom sufficiently automated, and therefore represent a bottleneck that can substantially offset performance benefits provided by even the most powerful modern supercomputers. Furthermore, a typical system architecture of modern supercomputing platforms is oriented towards the maximum computational performance and provides limited standard facilities for automation of the supporting activities. We present a prototype solution that automates all aspects of the seismic inversion process and is tuned for the modern massively parallel high performance computing systems. We address several major aspects of the solution architecture, which include (1) design of an inversion state database for tracing all relevant aspects of the entire solution process, (2) design of an extensible workflow management framework, (3) integration with wave propagation solvers, (4) integration with optimization packages, (5) computation of misfits and adjoint sources, and (6) process monitoring. The inversion state database represents a hierarchical structure with

  3. TNFRSF1B — EDRN Public Portal

    Cancer.gov

    TNFRSF1B, also known as TNFr2, a single-pass type I member of the TNF-receptor superfamily, forms a heterocomplex with TNF-receptor 1 that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Although the function of IAPs in TNF-receptor signalling is unknown, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest that TNFr2 plays a role in protecting neurons from apoptosis by stimulating antioxidative pathways.

  4. Wearable Accelerometers in High Performance Jet Aircraft.

    PubMed

    Rice, G Merrill; VanBrunt, Thomas B; Snider, Dallas H; Hoyt, Robert E

    2016-02-01

    Wearable accelerometers have become ubiquitous in the fields of exercise physiology and ambulatory hospital settings. However, these devices have yet to be validated in extreme operational environments. The objective of this study was to correlate the gravitational forces (G forces) detected by wearable accelerometers with the G forces detected by high performance aircraft. We compared the in-flight G forces detected by the two commercially available portable accelerometers to the F/A-18 Carrier Aircraft Inertial Navigation System (CAINS-2) during 20 flights performed by the Navy's Flight Demonstration Squadron (Blue Angels). Postflight questionnaires were also used to assess the perception of distractibility during flight. Of the 20 flights analyzed, 10 complete in-flight comparisons were made, accounting for 25,700 s of correlation between the CAINS-2 and the two tested accelerometers. Both accelerometers had strong correlations with that of the F/A-18 Gz axis, averaging r = 0.92 and r = 0.93, respectively, over 10 flights. Comparison of both portable accelerometer's average vector magnitude to each other yielded an average correlation of r = 0.93. Both accelerometers were found to be minimally distracting. These results suggest the use of wearable accelerometers is a valid means of detecting G forces during high performance aircraft flight. Future studies using this surrogate method of detecting accelerative forces combined with physiological information may yield valuable in-flight normative data that heretofore has been technically difficult to obtain and hence holds the promise of opening the door for a new golden age of aeromedical research.

  5. Association between the CYP1B1 polymorphisms and risk of cancer: a meta-analysis.

    PubMed

    Liu, Jie-Ying; Yang, Yu; Liu, Zhi-Zhong; Xie, Jian-Jun; Du, Ya-Ping; Wang, Wei

    2015-04-01

    The previous, published data on the association between CYP1B1 polymorphisms and cancer risk remained controversial. To derive a more precise estimation of the association between the CYP1B1 polymorphisms and cancer risk, we performed a meta-analysis to investigate the association between cancer susceptibility and CYP1B1 Leu432Val, Asn453Ser, Arg48Gly, and Ala119Ser polymorphisms. For Asn453Ser and Arg48Gly polymorphisms, significantly decreased endometrial cancer was observed among Caucasians. For Ala119Ser polymorphism, we found that individuals with the minor variant genotypes had a high risk of prostate cancer. For Leu432Val polymorphism, we found that individuals with the minor variant genotypes had a higher risk of endometrial cancer and lung cancer and had a lower risk of ovarian cancer. In summary, this meta-analysis suggests that Leu432Val polymorphism is associated with ovarian cancer, lung cancer, and endometrial cancer risk; Asn453Ser and Arg48Gly polymorphisms are associated with endometrial cancer risk among Caucasians, Ala119Ser polymorphism is associated with prostate cancer risk, and Ala119Ser polymorphism is associated with breast cancer risk in Caucasians. In addition, our work also points out the importance of new studies for Ala119Ser polymorphism in endometrial cancer, because high heterogeneity was observed (I (2) > 75 %).

  6. High performance electrospinning system for fabricating highly uniform polymer nanofibers

    NASA Astrophysics Data System (ADS)

    Munir, Muhammad Miftahul; Iskandar, Ferry; Khairurrijal, Okuyama, Kikuo

    2009-02-01

    A high performance electrospinning system has been successfully developed for production of highly uniform polymer nanofibers. The electrospinning system employed a proportional-integral-derivative control action to maintain a constant current during the production of polyvinyl acetate (PVAc) nanofibers from a precursor solution prepared by dissolution of the PVAc powder in dimethyl formamide so that high uniformity of the nanofibers was achieved. It was found that the cone jet length observed at the end of the needle during the injection of the precursor solution and the average diameter of the nanofibers decreased with decreasing Q /I, where Q is the flow rate of the precursor solution of the nanofibers and I is the current flowing through the electrospinning system. A power law obtained from the relation between the average diameter and Q /I is in accordance with the theoretical model.

  7. Characterization of a novel vasopressin V1b receptor antagonist, V1B-30N, in animal models of anxiety-like and depression-like behavior.

    PubMed

    Hodgson, Robert A; Mullins, Deborra; Lu, Sherry X; Guzzi, Mario; Zhang, Xiaoping; Bleickardt, Carina J; Scott, Jack D; Miller, Michael W; Stamford, Andrew W; Parker, Eric M; Varty, Geoffrey B

    2014-05-05

    Overactivity of the hypothalamic-pituitary-adrenal (HPA) axis has been linked to affective disorders such as anxiety and depression. Dampening HPA activity has, therefore, been considered as a possible means of treating affective disorders. Given the important role of vasopressin in modulating the HPA axis, one strategy has focused on inhibiting activity of the vasopressin 1b (V1b) receptor. In animals, V1b receptor antagonists reduce plasma stress hormone levels and have been shown to have an anxiolytic-like effect. Recently, V1B-30N was identified as a highly potent V1b receptor antagonist with selectivity over other vasopressin receptors, which is evaluated here in rodent models of anxiety-like and depression-like behaviors. V1B-30N (1-30mg/kg, IP) dose-dependently reduced separation-induced vocalizations in rat pups without producing any sedative effects in the animals. Similarly, V1B-30N (3-30mg/kg, IP) dose-dependently reduced separation-induced vocalizations in guinea pig pups. In a conflict assay, conditioned lick suppression, V1B-30N (3-30mg/kg, IP) increased punished licking. To assess antidepressive-like properties, V1B-30N (1-30mg/kg) was tested in the mouse and rat forced-swim tests but was found to be inactive. These results are consistent with previous findings with other V1b antagonists, which suggest that acute pharmacological antagonism of the V1b receptor has anxiolytic-like but not antidepressant-like properties. Copyright © 2014 Elsevier B.V. All rights reserved.

  8. Study of High-Performance Coronagraphic Techniques

    NASA Astrophysics Data System (ADS)

    Tolls, Volker; Aziz, M. J.; Gonsalves, R. A.; Korzennik, S. G.; Labeyrie, A.; Lyon, R. G.; Melnick, G. J.; Somerstein, S.; Vasudevan, G.; Woodruff, R. A.

    2007-05-01

    We will provide a progress report about our study of high-performance coronagraphic techniques. At SAO we have set up a testbed to test coronagraphic masks and to demonstrate Labeyrie's multi-step speckle reduction technique. This technique expands the general concept of a coronagraph by incorporating a speckle corrector (phase or amplitude) and second occulter for speckle light suppression. The testbed consists of a coronagraph with high precision optics (2 inch spherical mirrors with lambda/1000 surface quality), lasers simulating the host star and the planet, and a single Labeyrie correction stage with a MEMS deformable mirror (DM) for the phase correction. The correction function is derived from images taken in- and slightly out-of-focus using phase diversity. The testbed is operational awaiting coronagraphic masks. The testbed control software for operating the CCD camera, the translation stage that moves the camera in- and out-of-focus, the wavefront recovery (phase diversity) module, and DM control is under development. We are also developing coronagraphic masks in collaboration with Harvard University and Lockheed Martin Corp. (LMCO). The development at Harvard utilizes a focused ion beam system to mill masks out of absorber material and the LMCO approach uses patterns of dots to achieve the desired mask performance. We will present results of both investigations including test results from the first generation of LMCO masks obtained with our high-precision mask scanner. This work was supported by NASA through grant NNG04GC57G, through SAO IR&D funding, and by Harvard University through the Research Experience for Undergraduate Program of Harvard's Materials Science and Engineering Center. Central facilities were provided by Harvard's Center for Nanoscale Systems.

  9. PREFACE: High Performance Computing Symposium 2011

    NASA Astrophysics Data System (ADS)

    Talon, Suzanne; Mousseau, Normand; Peslherbe, Gilles; Bertrand, François; Gauthier, Pierre; Kadem, Lyes; Moitessier, Nicolas; Rouleau, Guy; Wittig, Rod

    2012-02-01

    HPCS (High Performance Computing Symposium) is a multidisciplinary conference that focuses on research involving High Performance Computing and its application. Attended by Canadian and international experts and renowned researchers in the sciences, all areas of engineering, the applied sciences, medicine and life sciences, mathematics, the humanities and social sciences, it is Canada's pre-eminent forum for HPC. The 25th edition was held in Montréal, at the Université du Québec à Montréal, from 15-17 June and focused on HPC in Medical Science. The conference was preceded by tutorials held at Concordia University, where 56 participants learned about HPC best practices, GPU computing, parallel computing, debugging and a number of high-level languages. 274 participants from six countries attended the main conference, which involved 11 invited and 37 contributed oral presentations, 33 posters, and an exhibit hall with 16 booths from our sponsors. The work that follows is a collection of papers presented at the conference covering HPC topics ranging from computer science to bioinformatics. They are divided here into four sections: HPC in Engineering, Physics and Materials Science, HPC in Medical Science, HPC Enabling to Explore our World and New Algorithms for HPC. We would once more like to thank the participants and invited speakers, the members of the Scientific Committee, the referees who spent time reviewing the papers and our invaluable sponsors. To hear the invited talks and learn about 25 years of HPC development in Canada visit the Symposium website: http://2011.hpcs.ca/lang/en/conference/keynote-speakers/ Enjoy the excellent papers that follow, and we look forward to seeing you in Vancouver for HPCS 2012! Gilles Peslherbe Chair of the Scientific Committee Normand Mousseau Co-Chair of HPCS 2011 Suzanne Talon Chair of the Organizing Committee UQAM Sponsors The PDF also contains photographs from the conference banquet.

  10. Scalable resource management in high performance computers.

    SciTech Connect

    Frachtenberg, E.; Petrini, F.; Fernandez Peinador, J.; Coll, S.

    2002-01-01

    Clusters of workstations have emerged as an important platform for building cost-effective, scalable and highly-available computers. Although many hardware solutions are available today, the largest challenge in making large-scale clusters usable lies in the system software. In this paper we present STORM, a resource management tool designed to provide scalability, low overhead and the flexibility necessary to efficiently support and analyze a wide range of job scheduling algorithms. STORM achieves these feats by closely integrating the management daemons with the low-level features that are common in state-of-the-art high-performance system area networks. The architecture of STORM is based on three main technical innovations. First, a sizable part of the scheduler runs in the thread processor located on the network interface. Second, we use hardware collectives that are highly scalable both for implementing control heartbeats and to distribute the binary of a parallel job in near-constant time, irrespective of job and machine sizes. Third, we use an I/O bypass protocol that allows fast data movements from the file system to the communication buffers in the network interface and vice versa. The experimental results show that STORM can launch a job with a binary of 12MB on a 64 processor/32 node cluster in less than 0.25 sec on an empty network, in less than 0.45 sec when all the processors are busy computing other jobs, and in less than 0.65 sec when the network is flooded with a background traffic. This paper provides experimental and analytical evidence that these results scale to a much larger number of nodes. To the best of our knowledge, STORM is at least two orders of magnitude faster than existing production schedulers in launching jobs, performing resource management tasks and gang scheduling.

  11. Low-Cost High-Performance MRI

    PubMed Central

    Sarracanie, Mathieu; LaPierre, Cristen D.; Salameh, Najat; Waddington, David E. J.; Witzel, Thomas; Rosen, Matthew S.

    2015-01-01

    Magnetic Resonance Imaging (MRI) is unparalleled in its ability to visualize anatomical structure and function non-invasively with high spatial and temporal resolution. Yet to overcome the low sensitivity inherent in inductive detection of weakly polarized nuclear spins, the vast majority of clinical MRI scanners employ superconducting magnets producing very high magnetic fields. Commonly found at 1.5–3 tesla (T), these powerful magnets are massive and have very strict infrastructure demands that preclude operation in many environments. MRI scanners are costly to purchase, site, and maintain, with the purchase price approaching $1 M per tesla (T) of magnetic field. We present here a remarkably simple, non-cryogenic approach to high-performance human MRI at ultra-low magnetic field, whereby modern under-sampling strategies are combined with fully-refocused dynamic spin control using steady-state free precession techniques. At 6.5 mT (more than 450 times lower than clinical MRI scanners) we demonstrate (2.5 × 3.5 × 8.5) mm3 imaging resolution in the living human brain using a simple, open-geometry electromagnet, with 3D image acquisition over the entire brain in 6 minutes. We contend that these practical ultra-low magnetic field implementations of MRI (<10 mT) will complement traditional MRI, providing clinically relevant images and setting new standards for affordable (<$50,000) and robust portable devices. PMID:26469756

  12. High performance visual display for HENP detectors

    NASA Astrophysics Data System (ADS)

    McGuigan, Michael; Smith, Gordon; Spiletic, John; Fine, Valeri; Nevski, Pavel

    2001-08-01

    A high end visual display for High Energy Nuclear Physics (HENP) detectors is necessary because of the sheer size and complexity of the detector. For BNL this display will be of special interest because of STAR and ATLAS. To load, rotate, query, and debug simulation code with a modern detector simply takes too long even on a powerful work station. To visualize the HENP detectors with maximal performance we have developed software with the following characteristics. We develop a visual display of HENP detectors on BNL multiprocessor visualization server at multiple level of detail. We work with general and generic detector framework consistent with ROOT, GAUDI etc, to avoid conflicting with the many graphic development groups associated with specific detectors like STAR and ATLAS. We develop advanced OpenGL features such as transparency and polarized stereoscopy. We enable collaborative viewing of detector and events by directly running the analysis in BNL stereoscopic theatre. We construct enhanced interactive control, including the ability to slice, search and mark areas of the detector. We incorporate the ability to make a high quality still image of a view of the detector and the ability to generate animations and a fly through of the detector and output these to MPEG or VRML models. We develop data compression hardware and software so that remote interactive visualization will be possible among dispersed collaborators. We obtain real time visual display for events accumulated during simulations.

  13. Low-Cost High-Performance MRI

    NASA Astrophysics Data System (ADS)

    Sarracanie, Mathieu; Lapierre, Cristen D.; Salameh, Najat; Waddington, David E. J.; Witzel, Thomas; Rosen, Matthew S.

    2015-10-01

    Magnetic Resonance Imaging (MRI) is unparalleled in its ability to visualize anatomical structure and function non-invasively with high spatial and temporal resolution. Yet to overcome the low sensitivity inherent in inductive detection of weakly polarized nuclear spins, the vast majority of clinical MRI scanners employ superconducting magnets producing very high magnetic fields. Commonly found at 1.5-3 tesla (T), these powerful magnets are massive and have very strict infrastructure demands that preclude operation in many environments. MRI scanners are costly to purchase, site, and maintain, with the purchase price approaching $1 M per tesla (T) of magnetic field. We present here a remarkably simple, non-cryogenic approach to high-performance human MRI at ultra-low magnetic field, whereby modern under-sampling strategies are combined with fully-refocused dynamic spin control using steady-state free precession techniques. At 6.5 mT (more than 450 times lower than clinical MRI scanners) we demonstrate (2.5 × 3.5 × 8.5) mm3 imaging resolution in the living human brain using a simple, open-geometry electromagnet, with 3D image acquisition over the entire brain in 6 minutes. We contend that these practical ultra-low magnetic field implementations of MRI (<10 mT) will complement traditional MRI, providing clinically relevant images and setting new standards for affordable (<$50,000) and robust portable devices.

  14. Fusion of HJ1B and ALOS PALSAR data for land cover classification using machine learning methods

    NASA Astrophysics Data System (ADS)

    Wang, X. Y.; Guo, Y. G.; He, J.; Du, L. T.

    2016-10-01

    Image classification from remote sensing is becoming increasingly urgent for monitoring environmental changes. Exploring effective algorithms to increase classification accuracy is critical. This paper explores the use of multispectral HJ1B and ALOS (Advanced Land Observing Satellite) PALSAR L-band (Phased Array type L-band Synthetic Aperture Radar) for land cover classification using learning-based algorithms. Pixel-based and object-based image analysis approaches for classifying HJ1B data and the HJ1B and ALOS/PALSAR fused-images were compared using two machine learning algorithms, support vector machine (SVM) and random forest (RF), to test which algorithm can achieve the best classification accuracy in arid and semiarid regions. The overall accuracies of the pixel-based (Fused data: 79.0%; HJ1B data: 81.46%) and object-based classifications (Fused data: 80.0%; HJ1B data: 76.9%) were relatively close when using the SVM classifier. The pixel-based classification achieved a high overall accuracy (85.5%) using the RF algorithm for classifying the fused data, whereas the RF classifier using the object-based image analysis produced a lower overall accuracy (70.2%). The study demonstrates that the pixel-based classification utilized fewer variables and performed relatively better than the object-based classification using HJ1B imagery and the fused data. Generally, the integration of the HJ1B and ALOS/PALSAR imagery can improve the overall accuracy of 5.7% using the pixel-based image analysis and RF classifier.

  15. Association between CYP1A2 and CYP1B1 Polymorphisms and Colorectal Cancer Risk: A Meta-Analysis

    PubMed Central

    Liu, Zhi-Zhong; Xie, Jian-Jun; Wang, Wei; Du, Ya-Ping; Chen, Yu; Si, Hui-Qiang; Liu, Qing; Wu, Li-Xia; Wei, Wu

    2014-01-01

    Background The previous published data on the association between CYP1A2*F (rs762551), CYP1B1 Leu432Val (rs1056836), Asn453Ser (rs180040), and Arg48Gly (rs10012) polymorphisms and colorectal cancer risk remained controversial. Methodology/Principal Findings The purpose of this study is to evaluate the role of CYP1A2*F, CYP1B1 Leu432Val, Asn453Ser, and Arg48Gly genotypes in colorectal cancer susceptibility. We performed a meta-analysis on all the eligible studies that provided 5,817 cases and 6,544 controls for CYP1A2*F (from 13 studies), 9219 cases and 10406 controls for CYP1B1 Leu432Val (from 12 studies), 6840 cases and 7761 controls for CYP1B1 Asn453Ser (from 8 studies), and 4302 cases and 4791 controls for CYP1B1Arg48Gly (from 6 studies). Overall, no significant association was found between CYP1A2*F, CYP1B1 Leu432Val, Asn453Ser, and Arg48Gly and colorectal cancer risk when all the eligible studies were pooled into the meta-analysis. And in the subgroup by ethnicity and source of controls, no evidence of significant association was observed in any subgroup analysis. Conclusions/Significance In summary, this meta-analysis indicates that CYP1A2*F, CYP1B1 Leu432Val, Asn453Ser, and Arg48Gly polymorphisms do not support an association with colorectal cancer, and further studies are needed to investigate the association. In addition, our work also points out the importance of new studies for CYP1A2*F polymorphism in Asians, because high heterogeneity was found (dominant model: I2 = 81.3%; heterozygote model: I2 = 79.0). PMID:25115775

  16. Thermal interface pastes nanostructured for high performance

    NASA Astrophysics Data System (ADS)

    Lin, Chuangang

    Thermal interface materials in the form of pastes are needed to improve thermal contacts, such as that between a microprocessor and a heat sink of a computer. High-performance and low-cost thermal pastes have been developed in this dissertation by using polyol esters as the vehicle and various nanoscale solid components. The proportion of a solid component needs to be optimized, as an excessive amount degrades the performance, due to the increase in the bond line thickness. The optimum solid volume fraction tends to be lower when the mating surfaces are smoother, and higher when the thermal conductivity is higher. Both a low bond line thickness and a high thermal conductivity help the performance. When the surfaces are smooth, a low bond line thickness can be even more important than a high thermal conductivity, as shown by the outstanding performance of the nanoclay paste of low thermal conductivity in the smooth case (0.009 mum), with the bond line thickness less than 1 mum, as enabled by low storage modulus G', low loss modulus G" and high tan delta. However, for rough surfaces, the thermal conductivity is important. The rheology affects the bond line thickness, but it does not correlate well with the performance. This study found that the structure of carbon black is an important parameter that governs the effectiveness of a carbon black for use in a thermal paste. By using a carbon black with a lower structure (i.e., a lower DBP value), a thermal paste that is more effective than the previously reported carbon black paste was obtained. Graphite nanoplatelet (GNP) was found to be comparable in effectiveness to carbon black (CB) pastes for rough surfaces, but it is less effective for smooth surfaces. At the same filler volume fraction, GNP gives higher thermal conductivity than carbon black paste. At the same pressure, GNP gives higher bond line thickness than CB (Tokai or Cabot). The effectiveness of GNP is limited, due to the high bond line thickness. A

  17. Integrating advanced facades into high performance buildings

    SciTech Connect

    Selkowitz, Stephen E.

    2001-05-01

    Glass is a remarkable material but its functionality is significantly enhanced when it is processed or altered to provide added intrinsic capabilities. The overall performance of glass elements in a building can be further enhanced when they are designed to be part of a complete facade system. Finally the facade system delivers the greatest performance to the building owner and occupants when it becomes an essential element of a fully integrated building design. This presentation examines the growing interest in incorporating advanced glazing elements into more comprehensive facade and building systems in a manner that increases comfort, productivity and amenity for occupants, reduces operating costs for building owners, and contributes to improving the health of the planet by reducing overall energy use and negative environmental impacts. We explore the role of glazing systems in dynamic and responsive facades that provide the following functionality: Enhanced sun protection and cooling load control while improving thermal comfort and providing most of the light needed with daylighting; Enhanced air quality and reduced cooling loads using natural ventilation schemes employing the facade as an active air control element; Reduced operating costs by minimizing lighting, cooling and heating energy use by optimizing the daylighting-thermal tradeoffs; Net positive contributions to the energy balance of the building using integrated photovoltaic systems; Improved indoor environments leading to enhanced occupant health, comfort and performance. In addressing these issues facade system solutions must, of course, respect the constraints of latitude, location, solar orientation, acoustics, earthquake and fire safety, etc. Since climate and occupant needs are dynamic variables, in a high performance building the facade solution have the capacity to respond and adapt to these variable exterior conditions and to changing occupant needs. This responsive performance capability

  18. Optics of high-performance electron microscopes*

    PubMed Central

    Rose, H H

    2008-01-01

    During recent years, the theory of charged particle optics together with advances in fabrication tolerances and experimental techniques has lead to very significant advances in high-performance electron microscopes. Here, we will describe which theoretical tools, inventions and designs have driven this development. We cover the basic theory of higher-order electron optics and of image formation in electron microscopes. This leads to a description of different methods to correct aberrations by multipole fields and to a discussion of the most advanced design that take advantage of these techniques. The theory of electron mirrors is developed and it is shown how this can be used to correct aberrations and to design energy filters. Finally, different types of energy filters are described. PMID:27877933

  19. High-performance nanostructured MR contrast probes

    PubMed Central

    Hu, Fengqin; Joshi, Hrushikesh M.; Dravid, Vinayak P.; Meade, Thomas J.

    2011-01-01

    Magnetic resonance imaging (MRI) has become a powerful technique in biological molecular imaging and clinical diagnosis. With the rapid progress in nanoscale science and technology, nanostructure-based MR contrast agents are undergoing rapid development. This is in part due to the tuneable magnetic and cellular uptake properties, large surface area for conjugation and favourable biodistribution. In this review, we describe our recent progress in the development of high-performance nanostructured MR contrast agents. Specifically, we report on Gd-enriched nanostructured probes that exhibit T1 MR contrast and superparamagnetic Fe3O4 and CoFe2O4 nanostructures that display T2 MR contrast enhancement. The effects of nanostructure size, shape, assembly and surface modification on relaxivity are described. The potential of these contrast agents for in vitro and in vivo MR imaging with respect to colloidal stability under physiological conditions, biocompatibility, and surface functionality are also evaluated. PMID:20694208

  20. High performance stepper motors for space mechanisms

    NASA Technical Reports Server (NTRS)

    Sega, Patrick; Estevenon, Christine

    1995-01-01

    Hybrid stepper motors are very well adapted to high performance space mechanisms. They are very simple to operate and are often used for accurate positioning and for smooth rotations. In order to fulfill these requirements, the motor torque, its harmonic content, and the magnetic parasitic torque have to be properly designed. Only finite element computations can provide enough accuracy to determine the toothed structures' magnetic permeance, whose derivative function leads to the torque. It is then possible to design motors with a maximum torque capability or with the most reduced torque harmonic content (less than 3 percent of fundamental). These later motors are dedicated to applications where a microstep or a synchronous mode is selected for minimal dynamic disturbances. In every case, the capability to convert electrical power into torque is much higher than on DC brushless motors.

  1. Optics of high-performance electron microscopes.

    PubMed

    Rose, H H

    2008-01-01

    During recent years, the theory of charged particle optics together with advances in fabrication tolerances and experimental techniques has lead to very significant advances in high-performance electron microscopes. Here, we will describe which theoretical tools, inventions and designs have driven this development. We cover the basic theory of higher-order electron optics and of image formation in electron microscopes. This leads to a description of different methods to correct aberrations by multipole fields and to a discussion of the most advanced design that take advantage of these techniques. The theory of electron mirrors is developed and it is shown how this can be used to correct aberrations and to design energy filters. Finally, different types of energy filters are described.

  2. High performance computing applications in neurobiological research

    NASA Technical Reports Server (NTRS)

    Ross, Muriel D.; Cheng, Rei; Doshay, David G.; Linton, Samuel W.; Montgomery, Kevin; Parnas, Bruce R.

    1994-01-01

    The human nervous system is a massively parallel processor of information. The vast numbers of neurons, synapses and circuits is daunting to those seeking to understand the neural basis of consciousness and intellect. Pervading obstacles are lack of knowledge of the detailed, three-dimensional (3-D) organization of even a simple neural system and the paucity of large scale, biologically relevant computer simulations. We use high performance graphics workstations and supercomputers to study the 3-D organization of gravity sensors as a prototype architecture foreshadowing more complex systems. Scaled-down simulations run on a Silicon Graphics workstation and scale-up, three-dimensional versions run on the Cray Y-MP and CM5 supercomputers.

  3. High performance stepper motors for space mechanisms

    NASA Astrophysics Data System (ADS)

    Sega, Patrick; Estevenon, Christine

    1995-05-01

    Hybrid stepper motors are very well adapted to high performance space mechanisms. They are very simple to operate and are often used for accurate positioning and for smooth rotations. In order to fulfill these requirements, the motor torque, its harmonic content, and the magnetic parasitic torque have to be properly designed. Only finite element computations can provide enough accuracy to determine the toothed structures' magnetic permeance, whose derivative function leads to the torque. It is then possible to design motors with a maximum torque capability or with the most reduced torque harmonic content (less than 3 percent of fundamental). These later motors are dedicated to applications where a microstep or a synchronous mode is selected for minimal dynamic disturbances. In every case, the capability to convert electrical power into torque is much higher than on DC brushless motors.

  4. High-performance Si microwire photovoltaics

    SciTech Connect

    Kelzenberg, Michael D.; Turner-Evans, Daniel B.; Putnam, Morgan C.; Boettcher, Shannon W.; Briggs, Ryan M.; Baek, Jae Y.; Lewis, Nathan S.; Atwater, Harry A.

    2011-01-07

    Crystalline Si wires, grown by the vapor–liquid–solid (VLS) process, have emerged as promising candidate materials for low-cost, thin-film photovoltaics. Here, we demonstrate VLS-grown Si microwires that have suitable electrical properties for high-performance photovoltaic applications, including long minority-carrier diffusion lengths (Ln>> 30 µm) and low surface recombination velocities (S << 70 cm·s-1). Single-wire radial p–n junction solar cells were fabricated with amorphous silicon and silicon nitride surface coatings, achieving up to 9.0% apparent photovoltaic efficiency, and exhibiting up to ~600 mV open-circuit voltage with over 80% fill factor. Projective single-wire measurements and optoelectronic simulations suggest that large-area Si wire-array solar cells have the potential to exceed 17% energy-conversion efficiency, offering a promising route toward cost-effective crystalline Si photovoltaics.

  5. BEDOPS: high-performance genomic feature operations

    PubMed Central

    Neph, Shane; Kuehn, M. Scott; Reynolds, Alex P.; Haugen, Eric; Thurman, Robert E.; Johnson, Audra K.; Rynes, Eric; Maurano, Matthew T.; Vierstra, Jeff; Thomas, Sean; Sandstrom, Richard; Humbert, Richard; Stamatoyannopoulos, John A.

    2012-01-01

    Summary: The large and growing number of genome-wide datasets highlights the need for high-performance feature analysis and data comparison methods, in addition to efficient data storage and retrieval techniques. We introduce BEDOPS, a software suite for common genomic analysis tasks which offers improved flexibility, scalability and execution time characteristics over previously published packages. The suite includes a utility to compress large inputs into a lossless format that can provide greater space savings and faster data extractions than alternatives. Availability: http://code.google.com/p/bedops/ includes binaries, source and documentation. Contact: sjn@u.washington.edu and jstam@u.washington.edu Supplementary information: Supplementary data are available at Bioinformatics online. PMID:22576172

  6. Study of High Performance Coronagraphic Techniques

    NASA Technical Reports Server (NTRS)

    Crane, Phil (Technical Monitor); Tolls, Volker

    2004-01-01

    The goal of the Study of High Performance Coronagraphic Techniques project (called CoronaTech) is: 1) to verify the Labeyrie multi-step speckle reduction method and 2) to develop new techniques to manufacture soft-edge occulter masks preferably with Gaussian absorption profile. In a coronagraph, the light from a bright host star which is centered on the optical axis in the image plane is blocked by an occulter centered on the optical axis while the light from a planet passes the occulter (the planet has a certain minimal distance from the optical axis). Unfortunately, stray light originating in the telescope and subsequent optical elements is not completely blocked causing a so-called speckle pattern in the image plane of the coronagraph limiting the sensitivity of the system. The sensitivity can be increased significantly by reducing the amount of speckle light. The Labeyrie multi-step speckle reduction method implements one (or more) phase correction steps to suppress the unwanted speckle light. In each step, the stray light is rephased and then blocked with an additional occulter which affects the planet light (or other companion) only slightly. Since the suppression is still not complete, a series of steps is required in order to achieve significant suppression. The second part of the project is the development of soft-edge occulters. Simulations have shown that soft-edge occulters show better performance in coronagraphs than hard-edge occulters. In order to utilize the performance gain of soft-edge occulters. fabrication methods have to be developed to manufacture these occulters according to the specification set forth by the sensitivity requirements of the coronagraph.

  7. Search for New High Performance Piezoelectrics

    NASA Astrophysics Data System (ADS)

    Ganesh, P.; Cohen, Ronald E.

    2008-03-01

    In an effort to design new high performance piezoelectric materials, we have performed first-principles calculations to study the energetics of several ABO3 type materials, with the `A' site being occupied by two types of atoms. The motivation comes from recent findings of a morphotropic-phase boundary, which gives rise to large electromechanical coupling, at high pressure and low temperatures in pure PbTiO3[1]. This prompted us to substitute the `A' site with a smaller atom compared to Pb to apply ``chemical pressure'' and tune the morphotropic-phase boundary to lower pressures. We have discovered (Pb1/2 Sn1/2)TiO3, (Pb1/2 Ge1/2)TiO3 and (Sn1/2 Ge1/2)TiO3 to be promising new piezoelectric materials. (Pb1/2 Sn1/2)TiO3 shows lower energy monoclinic phases (space groups Cm with polarization along [xxz] and Pm with polarization along [x0z]) compared to the layered tetragonal phase (space group P4mm with polarization along [001]) while the remaining two compounds have a rhombohedral (space group R3m with polarization along [111]) ground-state compared to the tetragonal phase in the rock-salt pattern (space group I4mm with polarization along [001]). Our results also suggest ease of polarization rotation and large electromechanical strain. [1] Zhigang Wu and Ronald E. Cohen, PRL, 95, 037601, 2005

  8. High-Performance Monopropellants and Catalysts Evaluated

    NASA Technical Reports Server (NTRS)

    Reed, Brian D.

    2004-01-01

    The NASA Glenn Research Center is sponsoring efforts to develop advanced monopropellant technology. The focus has been on monopropellant formulations composed of an aqueous solution of hydroxylammonium nitrate (HAN) and a fuel component. HAN-based monopropellants do not have a toxic vapor and do not need the extraordinary procedures for storage, handling, and disposal required of hydrazine (N2H4). Generically, HAN-based monopropellants are denser and have lower freezing points than N2H4. The performance of HAN-based monopropellants depends on the selection of fuel, the HAN-to-fuel ratio, and the amount of water in the formulation. HAN-based monopropellants are not seen as a replacement for N2H4 per se, but rather as a propulsion option in their own right. For example, HAN-based monopropellants would prove beneficial to the orbit insertion of small, power-limited satellites because of this propellant's high performance (reduced system mass), high density (reduced system volume), and low freezing point (elimination of tank and line heaters). Under a Glenn-contracted effort, Aerojet Redmond Rocket Center conducted testing to provide the foundation for the development of monopropellant thrusters with an I(sub sp) goal of 250 sec. A modular, workhorse reactor (representative of a 1-lbf thruster) was used to evaluate HAN formulations with catalyst materials. Stoichiometric, oxygen-rich, and fuelrich formulations of HAN-methanol and HAN-tris(aminoethyl)amine trinitrate were tested to investigate the effects of stoichiometry on combustion behavior. Aerojet found that fuelrich formulations degrade the catalyst and reactor faster than oxygen-rich and stoichiometric formulations do. A HAN-methanol formulation with a theoretical Isp of 269 sec (designated HAN269MEO) was selected as the baseline. With a combustion efficiency of at least 93 percent demonstrated for HAN-based monopropellants, HAN269MEO will meet the I(sub sp) 250 sec goal.

  9. The design of high-performance gliders

    NASA Technical Reports Server (NTRS)

    Mueller, B.; Heuermann, V.

    1985-01-01

    A high-performance glider is defined as a glider which has been designed to carry the pilot in a minimum of time a given distance, taking into account conditions which are as conveniently as possible. The present investigation has the objective to show approaches for enhancing the cross-country flight cruising speed, giving attention to the difficulties which the design engineer will have to overcome. The characteristics of the cross-country flight and their relation to the cruising speed are discussed, and a description is provided of mathematical expressions concerning the cruising speed, the sinking speed, and the optimum gliding speed. The effect of aspect ratio and wing loading on the cruising speed is illustrated with the aid of a graph. Trends in glider development are explored, taking into consideration the design of laminar profiles, the reduction of profile-related drag by plain flaps, and the variation of wing loading during the flight. A number of suggestions are made for obtaining gliders with improved performance.

  10. USING MULTITAIL NETWORKS IN HIGH PERFORMANCE CLUSTERS

    SciTech Connect

    S. COLL; E. FRACHTEMBERG; F. PETRINI; A. HOISIE; L. GURVITS

    2001-03-01

    Using multiple independent networks (also known as rails) is an emerging technique to overcome bandwidth limitations and enhance fault-tolerance of current high-performance clusters. We present and analyze various venues for exploiting multiple rails. Different rail access policies are presented and compared, including static and dynamic allocation schemes. An analytical lower bound on the number of networks required for static rail allocation is shown. We also present an extensive experimental comparison of the behavior of various allocation schemes in terms of bandwidth and latency. Striping messages over multiple rails can substantially reduce network latency, depending on average message size, network load and allocation scheme. The methods compared include a static rail allocation, a round-robin rail allocation, a dynamic allocation based on local knowledge, and a rail allocation that reserves both end-points of a message before sending it. The latter is shown to perform better than other methods at higher loads: up to 49% better than local-knowledge allocation and 37% better than the round-robin allocation. This allocation scheme also shows lower latency and it saturates on higher loads (for messages large enough). Most importantly, this proposed allocation scheme scales well with the number of rails and message sizes.

  11. High performance zinc air fuel cell stack

    NASA Astrophysics Data System (ADS)

    Pei, Pucheng; Ma, Ze; Wang, Keliang; Wang, Xizhong; Song, Mancun; Xu, Huachi

    2014-03-01

    A zinc air fuel cell (ZAFC) stack with inexpensive manganese dioxide (MnO2) as the catalyst is designed, in which the circulation flowing potassium hydroxide (KOH) electrolyte carries the reaction product away and acts as a coolant. Experiments are carried out to investigate the characteristics of polarization, constant current discharge and dynamic response, as well as the factors affecting the performance and uniformity of individual cells in the stack. The results reveal that the peak power density can be as high as 435 mW cm-2 according to the area of the air cathode sheet, and the influence factors on cell performance and uniformity are cell locations, filled state of zinc pellets, contact resistance, flow rates of electrolyte and air. It is also shown that the time needed for voltages to reach steady state and that for current step-up or current step-down are both in milliseconds, indicating the ZAFC can be excellently applied to vehicles with rapid dynamic response demands.

  12. Ultra High Performance, Highly Reliable, Numeric Intensive Processors and Systems

    DTIC Science & Technology

    1989-10-01

    to design high-performance DSP/IP systems using either off-the-shelf components or application specific integrated circuitry [ ASIC ]. -9 - HSDAL . ARO...are the chirp-z transform ( CZT ) [13] and (Rader’s) Prime Factor Transform (PFT) [11]. The RNS/ CZT is being studied by a group a MITRE [14] and is given...PFT RNS/CRNS/QRNS implementation has dynamic range requirements on the order of NQ2 (vs NQ4 for the CZT and much higher for the FFT). Therefore, the

  13. High performance internal reforming unit for high temperature fuel cells

    DOEpatents

    Ma, Zhiwen [Sandy Hook, CT; Venkataraman, Ramakrishnan [New Milford, CT; Novacco, Lawrence J [Brookfield, CT

    2008-10-07

    A fuel reformer having an enclosure with first and second opposing surfaces, a sidewall connecting the first and second opposing surfaces and an inlet port and an outlet port in the sidewall. A plate assembly supporting a catalyst and baffles are also disposed in the enclosure. A main baffle extends into the enclosure from a point of the sidewall between the inlet and outlet ports. The main baffle cooperates with the enclosure and the plate assembly to establish a path for the flow of fuel gas through the reformer from the inlet port to the outlet port. At least a first directing baffle extends in the enclosure from one of the sidewall and the main baffle and cooperates with the plate assembly and the enclosure to alter the gas flow path. Desired graded catalyst loading pattern has been defined for optimized thermal management for the internal reforming high temperature fuel cells so as to achieve high cell performance.

  14. High Performance Circularly Polarized Microstrip Antenna

    NASA Technical Reports Server (NTRS)

    Bondyopadhyay, Probir K. (Inventor)

    1997-01-01

    A microstrip antenna for radiating circularly polarized electromagnetic waves comprising a cluster array of at least four microstrip radiator elements, each of which is provided with dual orthogonal coplanar feeds in phase quadrature relation achieved by connection to an asymmetric T-junction power divider impedance notched at resonance. The dual fed circularly polarized reference element is positioned with its axis at a 45 deg angle with respect to the unit cell axis. The other three dual fed elements in the unit cell are positioned and fed with a coplanar feed structure with sequential rotation and phasing to enhance the axial ratio and impedance matching performance over a wide bandwidth. The centers of the radiator elements are disposed at the corners of a square with each side of a length d in the range of 0.7 to 0.9 times the free space wavelength of the antenna radiation and the radiator elements reside in a square unit cell area of sides equal to 2d and thereby permit the array to be used as a phased array antenna for electronic scanning and is realizable in a high temperature superconducting thin film material for high efficiency.

  15. High Performance Anion Chromatography of Gadolinium Chelates.

    PubMed

    Hajós, Peter; Lukács, Diana; Farsang, Evelin; Horváth, Krisztian

    2016-11-01

    High performance anion chromatography (HPIC) method to separate ionic Gd chelates, [Formula: see text], [Formula: see text], [Formula: see text] and free matrix anions was developed. At alkaline pHs, polydentate complexing agents such as ethylene-diamine-tetraacetate, diethylene-triamine pentaacetate and trans-1,2-diamine-cyclohexane-tetraacetate tend to form stable Gd chelate anions and can be separated by anion exchange. Separations were studied in the simple isocratic chromatographic run over the wide range of pH and concentration of carbonate eluent using suppressed conductivity detection. The ion exchange and complex forming equilibria were quantitatively described and demonstrated in order to understand major factors in the control of selectivity of Gd chelates. Parameters of optimized resolution between concurrent ions were presented on a 3D resolution surface. The applicability of the developed method is represented by the simultaneous analysis of Gd chelates and organic/inorganic anions. Inductively coupled plasma atomic emission spectroscopy  (ICP-AES) analysis was used for confirmation of HPIC results for Gd. Collection protocols for the heart-cutting procedure of chromatograms were applied. SPE procedures were also developed not only to extract traces of free gadolinium ions from samples, but also to remove the high level of interfering anions of the complex matrices. The limit of detection, the recoverability and the linearity of the method were also presented. © The Author 2016. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  16. High-performance computers for unmanned vehicles

    NASA Astrophysics Data System (ADS)

    Toms, David; Ettinger, Gil J.

    2005-10-01

    The present trend of increasing functionality onboard unmanned vehicles is made possible by rapid advances in high-performance computers (HPCs). An HPC is characterized by very high computational capability (100s of billions of operations per second) contained in lightweight, rugged, low-power packages. HPCs are critical to the processing of sensor data onboard these vehicles. Operations such as radar image formation, target tracking, target recognition, signal intelligence signature collection and analysis, electro-optic image compression, and onboard data exploitation are provided by these machines. The net effect of an HPC is to minimize communication bandwidth requirements and maximize mission flexibility. This paper focuses on new and emerging technologies in the HPC market. Emerging capabilities include new lightweight, low-power computing systems: multi-mission computing (using a common computer to support several sensors); onboard data exploitation; and large image data storage capacities. These new capabilities will enable an entirely new generation of deployed capabilities at reduced cost. New software tools and architectures available to unmanned vehicle developers will enable them to rapidly develop optimum solutions with maximum productivity and return on investment. These new technologies effectively open the trade space for unmanned vehicle designers.

  17. High performance diffuse reflectance circular dichroism spectrophotometer.

    PubMed

    Harada, Takunori; Miyoshi, Yuichi; Kuroda, Reiko

    2009-04-01

    A new dual-purpose transmittance circular dichroism (CD) and diffuse reflectance CD (DRCD) universal chiroptical spectrophotometer (UCS-3, J-800KCMFII) was successfully developed to have the capability of measuring DRCD spectra down to 190 nm with high efficiency, based on UCS-2: J-800KCMF. Optical components newly used in UCS-3 are the integrating sphere of optimum size and material to achieve high performance particularly in the shorter wavelength region, a baffle installed to reduce the first specular reflection signals, and a condenser lens to increase light intensity per area for small samples. As a result, UCS-3 has become a very powerful instrument to measure DRCD spectra of powdered samples in situ, with approximately 20 times sensitivity of existing UCS-2. We could show that to achieve similar quality DRCD spectra, only 50 mug of (S)-(+)-1,1(')-binaphthyl-2,2(')-diyl hydrogen phosphate is required on UCS-3, as compared with 1.12 mg for UCS-2.

  18. High performance BGMI circuit for VLWIR FPAs

    NASA Astrophysics Data System (ADS)

    Hao, Li-chao; Chen, Hong-lei; Huang, Ai-bo; Zhang, Jun-ling; Ding, Rui-jun

    2013-09-01

    An improved CMOS readout integrated circuit (ROIC) for N-on-P very long wavelength (VLWIR) detectors is designed, which has the ability to operate with a simple background suppression. It increases the integration time and the signal-to-noise ratio (SNR) of image data. A buffered gate modulation input (BGMI) cell as input circuit provides a low input resistance, high injection efficiency, and precise biasing voltage to the photodiode. By theoretically analyzing the characteristic parameters of MOS device at low temperature, a high gain's feedback amplifier is devised which using a differential stage to provide the inverting gain to improve linearity and to provide tight control of the detector bias. The final chip is fabricated with HHNEC 0.35um 1P4M process technology. The measurement results of the fabricated readout chip under 50K have successfully verified both readout function and performance improvement. With the 5.0V power supply, ROIC provides the output dynamic range over 2.5V. At the same time, the total power dissipation is less than 200mW, and the maximum readout speed is more than 2.5MHz.

  19. High-performance signal characterization workstation

    NASA Astrophysics Data System (ADS)

    Frampton, Keith R.

    1996-06-01

    Essex has been involved in quadratic processing research and the design of processors that compute these algorithms for the past 14 years. We are developing a more efficient processor (Labyrinth-IITM) that has higher dynamic range (greater than 100 dB) and enhanced throughput (approximately 70 times faster). Labyrinth-IITM is a unique half-rack integration of non-developmental units that provides the compute power to solve complex signal processing tasks with significantly reduced latency. The architecture is a flexible combination of high-speed laser optics and digital technologies that is readily configured by the customer to perform a variety of functions. One or two signals can be input to the processor for linear or quadratic processing. The new processor is much simpler, more compact, and more flexible than predecessors. This paper presents a description of this new workstation accelerator. The functions generated by this processor are the ambiguity function, Wigner-Ville function, and cyclic spectrum. Other functions that can be represented by two signal inputs can also be generated by this accelerator. Some applications include high resolution spectral analysis, radar waveform processing, signal detection and characterization, geolocation using time and frequency differences of arrival, and direction finding using angle of arrival.

  20. New high performance Si for optical devices

    NASA Astrophysics Data System (ADS)

    Tenma, T.; Matsuzaka, M.; Sako, R.; Takase, K.; Chiba, K.

    2016-05-01

    Against the backdrop of a growing demand in the areas of smart buildings, security, vehicle installation, and other applications, the market for far infrared cameras is expected to grow significantly in the future. However, since germanium (Ge) and chalcogenide glass, which have been used as the lens materials of far infrared cameras, are very expensive or highly toxic, there are some problems supporting the growing demand. We have therefore focused attention on silicon, which is inexpensive and less toxic. Although silicon has been used as a lens material of far infrared cameras, there are some problems remaining to be solved: Cz silicon is inexpensive but delivers low transmittance, and Fz silicon delivers sufficient transmittance but is expensive. We have developed New Cz silicon, which delivers high transmittance as Fz silicon does, and is inexpensive as conventional Cz silicon is. We have already started its sample work at both companies in Japan and overseas and have obtained excellent performance results. Mass production is scheduled to start in this fiscal year.

  1. NCI's Transdisciplinary High Performance Scientific Data Platform

    NASA Astrophysics Data System (ADS)

    Evans, Ben; Antony, Joseph; Bastrakova, Irina; Car, Nicholas; Cox, Simon; Druken, Kelsey; Evans, Bradley; Fraser, Ryan; Ip, Alex; Kemp, Carina; King, Edward; Minchin, Stuart; Larraondo, Pablo; Pugh, Tim; Richards, Clare; Santana, Fabiana; Smillie, Jon; Trenham, Claire; Wang, Jingbo; Wyborn, Lesley

    2016-04-01

    The Australian National Computational Infrastructure (NCI) manages Earth Systems data collections sourced from several domains and organisations onto a single High Performance Data (HPD) Node to further Australia's national priority research and innovation agenda. The NCI HPD Node has rapidly established its value, currently managing over 10 PBytes of datasets from collections that span a wide range of disciplines including climate, weather, environment, geoscience, geophysics, water resources and social sciences. Importantly, in order to facilitate broad user uptake, maximise reuse and enable transdisciplinary access through software and standardised interfaces, the datasets, associated information systems and processes have been incorporated into the design and operation of a unified platform that NCI has called, the National Environmental Research Data Interoperability Platform (NERDIP). The key goal of the NERDIP is to regularise data access so that it is easily discoverable, interoperable for different domains and enabled for high performance methods. It adopts and implements international standards and data conventions, and promotes scientific integrity within a high performance computing and data analysis environment. NCI has established a rich and flexible computing environment to access to this data, through the NCI supercomputer; a private cloud that supports both domain focused virtual laboratories and in-common interactive analysis interfaces; as well as remotely through scalable data services. Data collections of this importance must be managed with careful consideration of both their current use and the needs of the end-communities, as well as its future potential use, such as transitioning to more advanced software and improved methods. It is therefore critical that the data platform is both well-managed and trusted for stable production use (including transparency and reproducibility), agile enough to incorporate new technological advances and

  2. High Power Flex-Propellant Arcjet Performance

    NASA Technical Reports Server (NTRS)

    Litchford, Ron J.

    2011-01-01

    implied nearly frozen flow in the nozzle and yielded performance ranges of 800-1100 sec for hydrogen and 400-600 sec for ammonia. Inferred thrust-to-power ratios were in the range of 30-10 lbf/MWe for hydrogen and 60-20 lbf/MWe for ammonia. Successful completion of this test series represents a fundamental milestone in the progression of high power arcjet technology, and it is hoped that the results may serve as a reliable touchstone for the future development of MW-class regeneratively-cooled flex-propellant plasma rockets.

  3. Protein Tyrosine Phosphatase 1B (PTP1B): A Potential Target for Alzheimer’s Therapy?

    PubMed Central

    Vieira, Marcelo N. N.; Lyra e Silva, Natalia M.; Ferreira, Sergio T.; De Felice, Fernanda G.

    2017-01-01

    Despite significant advances in current understanding of mechanisms of pathogenesis in Alzheimer’s disease (AD), attempts at drug development based on those discoveries have failed to translate into effective, disease-modifying therapies. AD is a complex and multifactorial disease comprising a range of aberrant cellular/molecular processes taking part in different cell types and brain regions. As a consequence, therapeutics for AD should be able to block or compensate multiple abnormal pathological events. Here, we examine recent evidence that inhibition of protein tyrosine phosphatase 1B (PTP1B) may represent a promising strategy to combat a variety of AD-related detrimental processes. Besides its well described role as a negative regulator of insulin and leptin signaling, PTB1B recently emerged as a modulator of various other processes in the central nervous system (CNS) that are also implicated in AD. These include signaling pathways germane to learning and memory, regulation of synapse dynamics, endoplasmic reticulum (ER) stress and microglia-mediated neuroinflammation. We propose that PTP1B inhibition may represent an attractive and yet unexplored therapeutic approach to correct aberrant signaling pathways linked to AD. PMID:28197094

  4. 5-HT1B autoreceptor regulation of serotonin transporter activity in synaptosomes

    PubMed Central

    Hagan, Catherine E.; McDevitt, Ross A.; Liu, Yusha; Furay, Amy R.; Neumaier, John F.

    2012-01-01

    Serotonin-1B (5-HT1B) autoreceptors are located in serotonin (5-HT) terminals along with serotonin transporters (SERT), and play a critical role in autoregulation of serotonergic neurotransmission, and are implicated in disorders of serotonergic function, particularly emotional regulation. SERT modulates serotonergic neurotransmission by high-affinity reuptake of 5-HT. Alterations in SERT activity are associated with increased risk for depression and anxiety. Several neurotransmitter receptors are known to regulate SERT Km and Vmax, and previous work suggests that 5-HT1B autoreceptors may regulate 5-HT reuptake, in addition to modulating 5-HT release and synthesis. We used rotating disk electrode voltammetry to investigate 5-HT1B autoreceptor regulation of SERT-mediated 5-HT uptake into synaptosomes. The selective 5-HT1B antagonist SB224289 decreased SERT activity in synaptosomes prepared from wild-type but not 5-HT1B knockout mice, whereas SERT uptake was enhanced after pre-treatment with the selective 5-HT1B agonist CP94253. Furthermore, SERT activity varies as a function of 5-HT1B receptor expression—specifically, genetic deletion of 5-HT1B decreased SERT function, while viral-mediated overexpression of 5-HT1B autoreceptors in rat raphe neurons increased SERT activity in rat hippocampal synaptosomes. Considered collectively, these results provide evidence that 5-HT1B autoreceptors regulate SERT activity. Since SERT clearance rate varies as a function of 5-HT1B autoreceptor expression levels and is modulated by both activation and inhibition of 5-HT1B autoreceptors, this dynamic interaction may be an important mechanism of serotonin autoregulation with therapeutic implications. PMID:22961814

  5. Silicon Photomultiplier Performance in High ELectric Field

    NASA Astrophysics Data System (ADS)

    Montoya, J.; Morad, J.

    2016-12-01

    Roughly 27% of the universe is thought to be composed of dark matter. The Large Underground Xenon (LUX) relies on the emission of light from xenon atoms after a collision with a dark matter particle. After a particle interaction in the detector, two things can happen: the xenon will emit light and charge. The charge (electrons), in the liquid xenon needs to be pulled into the gas section so that it can interact with gas and emit light. This allows LUX to convert a single electron into many photons. This is done by applying a high voltage across the liquid and gas regions, effectively ripping electrons out of the liquid xenon and into the gas. The current device used to detect photons is the photomultiplier tube (PMT). These devices are large and costly. In recent years, a new technology that is capable of detecting single photons has emerged, the silicon photomultiplier (SiPM). These devices are cheaper and smaller than PMTs. Their performance in a high electric fields, such as those found in LUX, are unknown. It is possible that a large electric field could introduce noise on the SiPM signal, drowning the single photon detection capability. My hypothesis is that SiPMs will not observe a significant increase is noise at an electric field of roughly 10kV/cm (an electric field within the range used in detectors like LUX). I plan to test this hypothesis by first rotating the SiPMs with no applied electric field between two metal plates roughly 2 cm apart, providing a control data set. Then using the same angles test the dark counts with the constant electric field applied. Possibly the most important aspect of LUX, is the photon detector because it's what detects the signals. Dark matter is detected in the experiment by looking at the ratio of photons to electrons emitted for a given interaction in the detector. Interactions with a low electron to photon ratio are more like to be dark matter events than those with a high electron to photon ratio. The ability to

  6. Src regulates membrane trafficking of the Kv3.1b channel.

    PubMed

    Bae, Seong Han; Kim, Dong Hyun; Shin, Seok Kyo; Choi, Jin Sung; Park, Kang-Sik

    2014-01-03

    The Kv3.1 channel plays a crucial role in regulating the high-frequency firing properties of neurons. Here, we determined whether Src regulates the subcellular distributions of the Kv3.1b channel. Co-expression of active Src induced a dramatic redistribution of Kv3.1b to the endoplasmic reticulum. Furthermore, co-expression of the Kv3.1b channel with active Src induced a remarkable decrease in the pool of Kv3.1b at the cell surface. Moreover, the co-expression of active Src results in a significant decrease in the peak current densities of the Kv3.1b channel, and a substantial alteration in the voltage dependence of its steady-state inactivation. Taken together, these results indicate that Src kinase may play an important role in regulating membrane trafficking of Kv3.1b channels.

  7. T cell receptor recognition of CD1b presenting a mycobacterial glycolipid

    PubMed Central

    Gras, Stephanie; Van Rhijn, Ildiko; Shahine, Adam; Cheng, Tan-Yun; Bhati, Mugdha; Tan, Li Lynn; Halim, Hanim; Tuttle, Kathryn D.; Gapin, Laurent; Le Nours, Jérôme; Moody, D. Branch; Rossjohn, Jamie

    2016-01-01

    CD1 proteins present microbial lipids to T cells. Germline-encoded mycolyl lipid-reactive (GEM) T cells with conserved αβ T cell receptors (TCRs) recognize CD1b presenting mycobacterial mycolates. As the molecular basis underpinning TCR recognition of CD1b remains unknown, here we determine the structure of a GEM TCR bound to CD1b presenting glucose-6-O-monomycolate (GMM). The GEM TCR docks centrally above CD1b, whereby the conserved TCR α-chain extensively contacts CD1b and GMM. Through mutagenesis and study of T cells from tuberculosis patients, we identify a consensus CD1b footprint of TCRs present among GEM T cells. Using both the TCR α- and β-chains as tweezers to surround and grip the glucose moiety of GMM, GEM TCRs create a highly specific mechanism for recognizing this mycobacterial glycolipid. PMID:27807341

  8. Pharmacology of the selective 5-HT(1B/1D) agonist frovatriptan.

    PubMed

    Comer, M B

    2002-04-01

    To determine the pharmacological profile of frovatriptan. Frovatriptan is a new 5-HT(1B/1D) agonist developed for the treatment of migraine. Pharmacological studies were performed using in vitro and in vivo techniques. Radioligand-binding studies showed that frovatriptan has a high affinity for 5-HT(1B) and 5-HT(1D) receptors, and moderate affinity for 5-HT(1A), 5-HT(1F), and 5-HT(7) receptors. In vitro, frovatriptan acts as a potent full agonist at human cloned 5-HT(1B) and 5-HT(1D) receptors, and as a moderately potent full agonist at 5-HT(7) receptors. Studies of frovatriptan in isolated human arteries demonstrated a lower threshold for constriction of cerebral than coronary vasculature and a bell-shaped dose-response curve was apparent in the coronary arteries. In anesthetized dogs, frovatriptan administration produced no measurable effect on cardiac function or on blood pressure. Frovatriptan had no effects on coronary blood flow following transient coronary artery occlusion, whereas sumatriptan produced a prolonged and significant decrease in coronary blood flow. The pharmacology of frovatriptan suggests that it should be an effective agent for the acute treatment of migraine, with a low potential for undesirable peripheral effects.

  9. Evaluating performance of high efficiency mist eliminators

    SciTech Connect

    Waggoner, Charles A.; Parsons, Michael S.; Giffin, Paxton K.

    2013-07-01

    Processing liquid wastes frequently generates off gas streams with high humidity and liquid aerosols. Droplet laden air streams can be produced from tank mixing or sparging and processes such as reforming or evaporative volume reduction. Unfortunately these wet air streams represent a genuine threat to HEPA filters. High efficiency mist eliminators (HEME) are one option for removal of liquid aerosols with high dissolved or suspended solids content. HEMEs have been used extensively in industrial applications, however they have not seen widespread use in the nuclear industry. Filtering efficiency data along with loading curves are not readily available for these units and data that exist are not easily translated to operational parameters in liquid waste treatment plants. A specialized test stand has been developed to evaluate the performance of HEME elements under use conditions of a US DOE facility. HEME elements were tested at three volumetric flow rates using aerosols produced from an iron-rich waste surrogate. The challenge aerosol included submicron particles produced from Laskin nozzles and super micron particles produced from a hollow cone spray nozzle. Test conditions included ambient temperature and relative humidities greater than 95%. Data collected during testing HEME elements from three different manufacturers included volumetric flow rate, differential temperature across the filter housing, downstream relative humidity, and differential pressure (dP) across the filter element. Filter challenge was discontinued at three intermediate dPs and the filter to allow determining filter efficiency using dioctyl phthalate and then with dry surrogate aerosols. Filtering efficiencies of the clean HEME, the clean HEME loaded with water, and the HEME at maximum dP were also collected using the two test aerosols. Results of the testing included differential pressure vs. time loading curves for the nine elements tested along with the mass of moisture and solid

  10. The curing of high-performance concrete

    NASA Astrophysics Data System (ADS)

    Meeks, Kenneth Wayne

    This dissertation describes the latest information, technology, and research on the curing of high performance concrete (HPC). Expanded somewhat beyond the scope of HPC, it examines the current body of knowledge on the effects of various curing regimes on concrete. The significance and importance of curing are discussed as well as the various definitions of HPC. The current curing requirements, standards, and criteria as proposed by ACI, as well as those of other countries, are reviewed and discussed. The current prescriptive curing requirements may not be applicable to high performance concrete. The research program reported in this dissertation looked at one approach to development of curing criteria for this relatively new class of concrete. The program applies some of the basic concepts of the methodology developed by the German researcher, H. K. Hilsdorf, to the curing of HPC with the objective to determine minimum curing durations for adequate strength development. The approach is to determine what fraction of the standard-cured 28-day strength has to be attained at the end of the curing period to assure that the design strength is attained in the interior of the member. An innovative direct tension test was developed to measure the strength at specific depths from the drying surface of small mortar cylinders (50 x 127 mm (2 x 5 in.)). Two mortar mixtures were investigated, w/c = 0.30 and w/c = 0.45, and three different moist curing regimes, 1-day, 3-day, and 7-day. Specimens were stored in two environmental chambers at 25sp°C, 50% RH; and 25sp°C, 70% RH, until testing at the age of 28 days. Direct tensile tests were conducted using steel disks epoxied to the ends of the specimens. Also, the penetration of the drying front was calculated from the drying data using porosity and degree of hydration relationships. The major observation from these tests was that adequate strength is attained in both mortar mixtures with only one day of moist curing. The drying

  11. The Mechanism of Allosteric Inhibition of Protein Tyrosine Phosphatase 1B

    PubMed Central

    Lu, Shaoyong; Huang, Wenkang; Geng, Lv; Shen, Qiancheng; Zhang, Jian

    2014-01-01

    As the prototypical member of the PTP family, protein tyrosine phosphatase 1B (PTP1B) is an attractive target for therapeutic interventions in type 2 diabetes. The extremely conserved catalytic site of PTP1B renders the design of selective PTP1B inhibitors intractable. Although discovered allosteric inhibitors containing a benzofuran sulfonamide scaffold offer fascinating opportunities to overcome selectivity issues, the allosteric inhibitory mechanism of PTP1B has remained elusive. Here, molecular dynamics (MD) simulations, coupled with a dynamic weighted community analysis, were performed to unveil the potential allosteric signal propagation pathway from the allosteric site to the catalytic site in PTP1B. This result revealed that the allosteric inhibitor compound-3 induces a conformational rearrangement in helix α7, disrupting the triangular interaction among helix α7, helix α3, and loop11. Helix α7 then produces a force, pulling helix α3 outward, and promotes Ser190 to interact with Tyr176. As a result, the deviation of Tyr176 abrogates the hydrophobic interactions with Trp179 and leads to the downward movement of the WPD loop, which forms an H-bond between Asp181 and Glu115. The formation of this H-bond constrains the WPD loop to its open conformation and thus inactivates PTP1B. The discovery of this allosteric mechanism provides an overall view of the regulation of PTP1B, which is an important insight for the design of potent allosteric PTP1B inhibitors. PMID:24831294

  12. High performance hand-held gas chromatograph

    SciTech Connect

    Yu, C.M.

    1998-04-28

    The Microtechnology Center of Lawrence Livermore National Laboratory has developed a high performance hand-held, real time detection gas chromatograph (HHGC) by Micro-Electro-Mechanical-System (MEMS) technology. The total weight of this hand-held gas chromatograph is about five lbs., with a physical size of 8{close_quotes} x 5{close_quotes} x 3{close_quotes} including carrier gas and battery. It consumes about 12 watts of electrical power with a response time on the order of one to two minutes. This HHGC has an average effective theoretical plate of about 40k. Presently, its sensitivity is limited by its thermal sensitive detector at PPM. Like a conventional G.C., this HHGC consists mainly of three major components: (1) the sample injector, (2) the column, and (3) the detector with related electronics. The present HHGC injector is a modified version of the conventional injector. Its separation column is fabricated completely on silicon wafers by means of MEMS technology. This separation column has a circular cross section with a diameter of 100 pm. The detector developed for this hand-held GC is a thermal conductivity detector fabricated on a silicon nitride window by MEMS technology. A normal Wheatstone bridge is used. The signal is fed into a PC and displayed through LabView software.

  13. An integrated high performance Fastbus slave interface

    SciTech Connect

    Christiansen, J.; Ljuslin, C. )

    1993-08-01

    A high performance CMOS Fastbus slave interface ASIC (Application Specific Integrated Circuit) supporting all addressing and data transfer modes defined in the IEEE 960 - 1986 standard is presented. The FAstbus Slave Integrated Circuit (FASIC) is an interface between the asynchronous Fastbus and a clock synchronous processor/memory bus. It can work stand-alone or together with a 32 bit microprocessor. The FASIC is a programmable device enabling its direct use in many different applications. A set of programmable address mapping windows can map Fastbus addresses to convenient memory addresses and at the same time act as address decoding logic. Data rates of 100 MBytes/sec to Fastbus can be obtained using an internal FIFO in the FASIC to buffer data between the two buses during block transfers. Message passing from Fastbus to a microprocessor on the slave module is supported. A compact (70 mm x 170 mm) Fastbus slave piggy back sub-card interface including level conversion between ECL and TTL signal levels has been implemented using surface mount components and the 208 pin FASIC chip.

  14. High performance techniques for space mission scheduling

    NASA Technical Reports Server (NTRS)

    Smith, Stephen F.

    1994-01-01

    In this paper, we summarize current research at Carnegie Mellon University aimed at development of high performance techniques and tools for space mission scheduling. Similar to prior research in opportunistic scheduling, our approach assumes the use of dynamic analysis of problem constraints as a basis for heuristic focusing of problem solving search. This methodology, however, is grounded in representational assumptions more akin to those adopted in recent temporal planning research, and in a problem solving framework which similarly emphasizes constraint posting in an explicitly maintained solution constraint network. These more general representational assumptions are necessitated by the predominance of state-dependent constraints in space mission planning domains, and the consequent need to integrate resource allocation and plan synthesis processes. First, we review the space mission problems we have considered to date and indicate the results obtained in these application domains. Next, we summarize recent work in constraint posting scheduling procedures, which offer the promise of better future solutions to this class of problems.

  15. High performance vapour-cell frequency standards

    NASA Astrophysics Data System (ADS)

    Gharavipour, M.; Affolderbach, C.; Kang, S.; Bandi, T.; Gruet, F.; Pellaton, M.; Mileti, G.

    2016-06-01

    We report our investigations on a compact high-performance rubidium (Rb) vapour-cell clock based on microwave-optical double-resonance (DR). These studies are done in both DR continuous-wave (CW) and Ramsey schemes using the same Physics Package (PP), with the same Rb vapour cell and a magnetron-type cavity with only 45 cm3 external volume. In the CW-DR scheme, we demonstrate a DR signal with a contrast of 26% and a linewidth of 334 Hz; in Ramsey-DR mode Ramsey signals with higher contrast up to 35% and a linewidth of 160 Hz have been demonstrated. Short-term stabilities of 1.4×10-13 τ-1/2 and 2.4×10-13 τ-1/2 are measured for CW-DR and Ramsey-DR schemes, respectively. In the Ramsey-DR operation, thanks to the separation of light and microwave interactions in time, the light-shift effect has been suppressed which allows improving the long-term clock stability as compared to CW-DR operation. Implementations in miniature atomic clocks are considered.

  16. High Performance Graphene Oxide Based Rubber Composites

    NASA Astrophysics Data System (ADS)

    Mao, Yingyan; Wen, Shipeng; Chen, Yulong; Zhang, Fazhong; Panine, Pierre; Chan, Tung W.; Zhang, Liqun; Liang, Yongri; Liu, Li

    2013-08-01

    In this paper, graphene oxide/styrene-butadiene rubber (GO/SBR) composites with complete exfoliation of GO sheets were prepared by aqueous-phase mixing of GO colloid with SBR latex and a small loading of butadiene-styrene-vinyl-pyridine rubber (VPR) latex, followed by their co-coagulation. During co-coagulation, VPR not only plays a key role in the prevention of aggregation of GO sheets but also acts as an interface-bridge between GO and SBR. The results demonstrated that the mechanical properties of the GO/SBR composite with 2.0 vol.% GO is comparable with those of the SBR composite reinforced with 13.1 vol.% of carbon black (CB), with a low mass density and a good gas barrier ability to boot. The present work also showed that GO-silica/SBR composite exhibited outstanding wear resistance and low-rolling resistance which make GO-silica/SBR very competitive for the green tire application, opening up enormous opportunities to prepare high performance rubber composites for future engineering applications.

  17. High Performance Graphene Oxide Based Rubber Composites

    PubMed Central

    Mao, Yingyan; Wen, Shipeng; Chen, Yulong; Zhang, Fazhong; Panine, Pierre; Chan, Tung W.; Zhang, Liqun; Liang, Yongri; Liu, Li

    2013-01-01

    In this paper, graphene oxide/styrene-butadiene rubber (GO/SBR) composites with complete exfoliation of GO sheets were prepared by aqueous-phase mixing of GO colloid with SBR latex and a small loading of butadiene-styrene-vinyl-pyridine rubber (VPR) latex, followed by their co-coagulation. During co-coagulation, VPR not only plays a key role in the prevention of aggregation of GO sheets but also acts as an interface-bridge between GO and SBR. The results demonstrated that the mechanical properties of the GO/SBR composite with 2.0 vol.% GO is comparable with those of the SBR composite reinforced with 13.1 vol.% of carbon black (CB), with a low mass density and a good gas barrier ability to boot. The present work also showed that GO-silica/SBR composite exhibited outstanding wear resistance and low-rolling resistance which make GO-silica/SBR very competitive for the green tire application, opening up enormous opportunities to prepare high performance rubber composites for future engineering applications. PMID:23974435

  18. High performance graphene oxide based rubber composites.

    PubMed

    Mao, Yingyan; Wen, Shipeng; Chen, Yulong; Zhang, Fazhong; Panine, Pierre; Chan, Tung W; Zhang, Liqun; Liang, Yongri; Liu, Li

    2013-01-01

    In this paper, graphene oxide/styrene-butadiene rubber (GO/SBR) composites with complete exfoliation of GO sheets were prepared by aqueous-phase mixing of GO colloid with SBR latex and a small loading of butadiene-styrene-vinyl-pyridine rubber (VPR) latex, followed by their co-coagulation. During co-coagulation, VPR not only plays a key role in the prevention of aggregation of GO sheets but also acts as an interface-bridge between GO and SBR. The results demonstrated that the mechanical properties of the GO/SBR composite with 2.0 vol.% GO is comparable with those of the SBR composite reinforced with 13.1 vol.% of carbon black (CB), with a low mass density and a good gas barrier ability to boot. The present work also showed that GO-silica/SBR composite exhibited outstanding wear resistance and low-rolling resistance which make GO-silica/SBR very competitive for the green tire application, opening up enormous opportunities to prepare high performance rubber composites for future engineering applications.

  19. New cis-regulatory elements in the Rht-D1b locus region of wheat

    USDA-ARS?s Scientific Manuscript database

    Fifteen gene-containing BACs with accumulated length of 1.82-Mb from the Rht-D1b locus region weresequenced and compared in detail with the orthologous regions of rice, sorghum, and maize. Our results show that Rht-D1b represents a conserved genomic region as implied by high gene sequence identity...

  20. Interleukin 1B variant -1473G/C (rs1143623) influences triglyceride and interleukin 6 metabolism

    USDA-ARS?s Scientific Manuscript database

    Interleukin 1b (IL1B or IL-1ß), is a key modulator of the immune response which exerts its functions mainly via interleukin 6 (IL6) regulation. Fatty meals cause transient hypertriglyceridemia and are considered to be proinflammatory, but the extent of these responses shows high interindividual susc...

  1. Cytochrome P1B1 (CYP1B1) polymorphisms and cancer risk: a meta-analysis of 52 studies.

    PubMed

    Li, Cuiping; Long, Bingshuang; Qin, Xianjing; Li, Weixiong; Zhou, Yang

    2015-01-02

    CYP1B1 plays a critical role in the oxidative metabolism of a variety of exogenous compounds, including carcinogenic compounds, which may be activated during metabolism. There are only a few studies that have examined the association between the two polymorphisms and cancer, and that these studies have been inconclusive. Hence, the aim of the present meta-analysis was to evaluate the relationship between polymorphisms in CYP1B1 G119T and A453G and cancer risk. We performed a detailed search using the PubMed and EMBASE libraries to obtain all relevant published reports on the relationship between the G119T and A453G polymorphisms in CYP1B1 and cancer risk. Combined odds ratios (ORs) and corresponding 95% confidence intervals (CIs) were calculated by Stata version 11.2. We conducted stratified analyses based on cancer types, ethnicity, source of controls, and quality assessments. We also made assessments of heterogeneity tests, sensitivity analyses, and publication bias. There were a total of 25 articles with 15,376 cases and 18,382 controls concerning CYP1B1 G119T and 40 articles with 27,983 cases and 35,839 controls concerning A453G polymorphisms. Regarding G119T, the combined results indicate that the variant genotypes were significantly associated with a slightly increased cancer risk in comparison to the homozygote (TT versus GG: p=0.006, OR=1.231, 95% CI: 1.061-1.428), especially for breast cancer and prostate cancer. Moreover, significantly increased associations with cancer risk were demonstrated in Asians in all genetic models. The combined results indicated no association of A453G with cancer risk; however, an association was observed specifically for prostate cancer. This meta-analysis suggests that the CYP1B1 G119T polymorphism may confer to genetic susceptibility to cancer in Asians, especially to breast cancer and prostate cancer. The A453G polymorphism was found to modify the risk of prostate cancer.

  2. Kinetics and Mechanism of Protein Tyrosine Phosphatase 1B (PTP1B) Inactivation by Acrolein

    PubMed Central

    Seiner, Derrick R.; LaButti, Jason N.; Gates, Kent S.

    2010-01-01

    Human cells are exposed to the electrophilic α,β-unsaturated aldehyde acrolein from a variety of sources. Reaction of acrolein with functionally critical protein thiol residues can yield important biological consequences. Protein tyrosine phosphatases (PTPs) are an important class of cysteine-dependent enzymes whose reactivity with acrolein previously has not been well characterized. These enzymes catalyze the dephosphorylation of phosphotyrosine residues on proteins via a phosphocysteine intermediate. PTPs work in tandem with protein tyrosine kinases to regulate a number of critically important mammalian signal transduction pathways. We find that acrolein is a potent time-dependent inactivator of the enzyme PTP1B (kinact = 0.02 ± 0.005 s−1, KI = 2.3 ± 0.6 × 10−4 M). Enzyme activity does not return upon gel filtration of the inactivated enzyme and addition of the competitive phosphatase inhibitor vanadate slows inactivation of PTP1B by acrolein. Together these observations suggest that acrolein covalently modifies the active site of PTP1B. Mass spectrometric analysis reveals that acrolein modifies the catalytic cysteine residue at the active site of the enzyme. Aliphatic aldehydes such as glyoxal, acetaldehyde, and propanal are relatively weak inactivators of PTP1B under the conditions employed here. Similarly, unsaturated aldehydes such as crotonaldehyde and 3-methyl-2-butenal bearing substitution at the alkene terminus are poor inactivators of the enzyme. Overall, the data suggest that enzyme inactivation occurs via conjugate addition of the catalytic cysteine residue to the carbon-carbon double bond of acrolein. The results indicate that inactivation of PTPs should be considered as a possible contributor to the diverse biological activities of acrolein and structurally-related α,β-unsaturated aldehydes. PMID:17655273

  3. Mutations in STX1B, encoding a presynaptic protein, cause fever-associated epilepsy syndromes.

    PubMed

    Schubert, Julian; Siekierska, Aleksandra; Langlois, Mélanie; May, Patrick; Huneau, Clément; Becker, Felicitas; Muhle, Hiltrud; Suls, Arvid; Lemke, Johannes R; de Kovel, Carolien G F; Thiele, Holger; Konrad, Kathryn; Kawalia, Amit; Toliat, Mohammad R; Sander, Thomas; Rüschendorf, Franz; Caliebe, Almuth; Nagel, Inga; Kohl, Bernard; Kecskés, Angela; Jacmin, Maxime; Hardies, Katia; Weckhuysen, Sarah; Riesch, Erik; Dorn, Thomas; Brilstra, Eva H; Baulac, Stephanie; Møller, Rikke S; Hjalgrim, Helle; Koeleman, Bobby P C; Jurkat-Rott, Karin; Lehman-Horn, Frank; Roach, Jared C; Glusman, Gustavo; Hood, Leroy; Galas, David J; Martin, Benoit; de Witte, Peter A M; Biskup, Saskia; De Jonghe, Peter; Helbig, Ingo; Balling, Rudi; Nürnberg, Peter; Crawford, Alexander D; Esguerra, Camila V; Weber, Yvonne G; Lerche, Holger

    2014-12-01

    Febrile seizures affect 2-4% of all children and have a strong genetic component. Recurrent mutations in three main genes (SCN1A, SCN1B and GABRG2) have been identified that cause febrile seizures with or without epilepsy. Here we report the identification of mutations in STX1B, encoding syntaxin-1B, that are associated with both febrile seizures and epilepsy. Whole-exome sequencing in independent large pedigrees identified cosegregating STX1B mutations predicted to cause an early truncation or an in-frame insertion or deletion. Three additional nonsense or missense mutations and a de novo microdeletion encompassing STX1B were then identified in 449 familial or sporadic cases. Video and local field potential analyses of zebrafish larvae with antisense knockdown of stx1b showed seizure-like behavior and epileptiform discharges that were highly sensitive to increased temperature. Wild-type human syntaxin-1B but not a mutated protein rescued the effects of stx1b knockdown in zebrafish. Our results thus implicate STX1B and the presynaptic release machinery in fever-associated epilepsy syndromes.

  4. Cyclin-dependent kinase inhibitor 1B (CDKN1B) gene variants in AIP mutation-negative familial isolated pituitary adenoma kindreds.

    PubMed

    Tichomirowa, Maria A; Lee, Misu; Barlier, Anne; Daly, Adrian F; Marinoni, Ilaria; Jaffrain-Rea, Marie-Lise; Naves, Luciana A; Rodien, Patrice; Rohmer, Vincent; Faucz, Fabio Rueda; Caron, Philippe; Estour, Bruno; Lecomte, Pierre; Borson-Chazot, Françoise; Penfornis, Alfred; Yaneva, Maria; Guitelman, Mirtha; Castermans, Emily; Verhaege, Catherine; Wémeau, Jean-Louis; Tabarin, Antoine; Fajardo Montañana, Carmen; Delemer, Brigitte; Kerlan, Veronique; Sadoul, Jean-Louis; Cortet Rudelli, Christine; Archambeaud, Françoise; Zacharieva, Sabine; Theodoropoulou, Marily; Brue, Thierry; Enjalbert, Alain; Bours, Vincent; Pellegata, Natalia S; Beckers, Albert

    2012-06-01

    Familial isolated pituitary adenoma (FIPA) occurs in families and is unrelated to multiple endocrine neoplasia type 1 and Carney complex. Mutations in AIP account only for 15-25% of FIPA families. CDKN1B mutations cause MEN4 in which affected patients can suffer from pituitary adenomas. With this study, we wanted to assess whether mutations in CDKN1B occur among a large cohort of AIP mutation-negative FIPA kindreds. Eighty-eight AIP mutation-negative FIPA families were studied and 124 affected subjects underwent sequencing of CDKN1B. Functional analysis of putative CDKN1B mutations was performed using in silico and in vitro approaches. Germline CDKN1B analysis revealed two nucleotide changes: c.286A>C (p.K96Q) and c.356T>C (p.I119T). In vitro, the K96Q change decreased p27 affinity for Grb2 but did not segregate with pituitary adenoma in the FIPA kindred. The I119T substitution occurred in a female patient with acromegaly. p27(I119T) shows an abnormal migration pattern by SDS-PAGE. Three variants (p.S56T, p.T142T, and c.605+36C>T) are likely nonpathogenic because In vitro effects were not seen. In conclusion, two patients had germline sequence changes in CDKN1B, which led to functional alterations in the encoded p27 proteins in vitro. Such rare CDKN1B variants may contribute to the development of pituitary adenomas, but their low incidence and lack of clear segregation with affected patients make CDKN1B sequencing unlikely to be of use in routine genetic investigation of FIPA kindreds. However, further characterization of the role of CDKN1B in pituitary tumorigenesis in these and other cases could help clarify the clinicopathological profile of MEN4.

  5. Practical route to the left wing of CTX1B and total syntheses of CTX1B and 54-deoxyCTX1B.

    PubMed

    Yamashita, Shuji; Takeuchi, Katsutoshi; Koyama, Takuya; Inoue, Masayuki; Hayashi, Yujiro; Hirama, Masahiro

    2015-02-02

    Ciguatoxins, the principal causative agents of ciguatera seafood poisoning, are extremely large polycyclic ethers. We report herein a reliable route for constructing the left wing of CTX1B, which possesses the acid/base/oxidant-sensitive bisallylic ether moiety, by a 6-exo radical cyclization/ring-closing metathesis strategy. This new route enabled us to achieve the second-generation total synthesis of CTX1B and the first synthesis of 54-deoxyCTX1B.

  6. Flow simulation and high performance computing

    NASA Astrophysics Data System (ADS)

    Tezduyar, T.; Aliabadi, S.; Behr, M.; Johnson, A.; Kalro, V.; Litke, M.

    1996-10-01

    Flow simulation is a computational tool for exploring science and technology involving flow applications. It can provide cost-effective alternatives or complements to laboratory experiments, field tests and prototyping. Flow simulation relies heavily on high performance computing (HPC). We view HPC as having two major components. One is advanced algorithms capable of accurately simulating complex, real-world problems. The other is advanced computer hardware and networking with sufficient power, memory and bandwidth to execute those simulations. While HPC enables flow simulation, flow simulation motivates development of novel HPC techniques. This paper focuses on demonstrating that flow simulation has come a long way and is being applied to many complex, real-world problems in different fields of engineering and applied sciences, particularly in aerospace engineering and applied fluid mechanics. Flow simulation has come a long way because HPC has come a long way. This paper also provides a brief review of some of the recently-developed HPC methods and tools that has played a major role in bringing flow simulation where it is today. A number of 3D flow simulations are presented in this paper as examples of the level of computational capability reached with recent HPC methods and hardware. These examples are, flow around a fighter aircraft, flow around two trains passing in a tunnel, large ram-air parachutes, flow over hydraulic structures, contaminant dispersion in a model subway station, airflow past an automobile, multiple spheres falling in a liquid-filled tube, and dynamics of a paratrooper jumping from a cargo aircraft.

  7. Experience with high-performance PACS

    NASA Astrophysics Data System (ADS)

    Wilson, Dennis L.; Goldburgh, Mitchell M.; Head, Calvin

    1997-05-01

    Lockheed Martin (Loral) has installed PACS with associated teleradiology in several tens of hospitals. The PACS that have been installed have been the basis for a shift to filmless radiology in many of the hospitals. the basic structure for the PACS and the teleradiology that is being used is outlined. The way that the PACS are being used in the hospitals is instructive. The three most used areas for radiology in the hospital are the wards including the ICU wards, the emergency room, and the orthopedics clinic. The examinations are mostly CR images with 20 percent to 30 percent of the examinations being CT, MR, and ultrasound exams. The PACS are being used to realize improved productivity for radiology and for the clinicians. For radiology the same staff is being used for 30 to 50 percent more workload. For the clinicians 10 to 20 percent of their time is being saved in dealing with radiology images. The improved productivity stems from the high performance of the PACS that has been designed and installed. Images are available on any workstation in the hospital within less than two seconds, even during the busiest hour of the day. The examination management functions to restrict the attention of any one user to the examinations that are of interest. The examination management organizes the workflow through the radiology department and the hospital, improving the service of the radiology department by reducing the time until the information from a radiology examination is available. The remaining weak link in the PACS system is transcription. The examination can be acquired, read, an the report dictated in much less than ten minutes. The transcription of the dictated reports can take from a few hours to a few days. The addition of automatic transcription services will remove this weak link.

  8. High-performance commercial building systems

    SciTech Connect

    Selkowitz, Stephen

    2003-10-01

    This report summarizes key technical accomplishments resulting from the three year PIER-funded R&D program, ''High Performance Commercial Building Systems'' (HPCBS). The program targets the commercial building sector in California, an end-use sector that accounts for about one-third of all California electricity consumption and an even larger fraction of peak demand, at a cost of over $10B/year. Commercial buildings also have a major impact on occupant health, comfort and productivity. Building design and operations practices that influence energy use are deeply engrained in a fragmented, risk-averse industry that is slow to change. Although California's aggressive standards efforts have resulted in new buildings designed to use less energy than those constructed 20 years ago, the actual savings realized are still well below technical and economic potentials. The broad goal of this program is to develop and deploy a set of energy-saving technologies, strategies, and techniques, and improve processes for designing, commissioning, and operating commercial buildings, while improving health, comfort, and performance of occupants, all in a manner consistent with sound economic investment practices. Results are to be broadly applicable to the commercial sector for different building sizes and types, e.g. offices and schools, for different classes of ownership, both public and private, and for owner-occupied as well as speculative buildings. The program aims to facilitate significant electricity use savings in the California commercial sector by 2015, while assuring that these savings are affordable and promote high quality indoor environments. The five linked technical program elements contain 14 projects with 41 distinct R&D tasks. Collectively they form a comprehensive Research, Development, and Demonstration (RD&D) program with the potential to capture large savings in the commercial building sector, providing significant economic benefits to building owners and

  9. P27/CDKN1B Translational Regulators in Pituitary Tumorigenesis.

    PubMed

    Martins, C S; Camargo, R C; Saggioro, F P; Neder, L; Machado, H R; Moreira, A C; de Castro, M

    2016-12-01

    In pituitary tumors, P27(CDKN1B) is underexpressed. We aimed to clarify whether translational regulation underlies this phenomenon. This study evaluated the expression of P27/CDKN1B, its targets (CCNE1, CDK2) and translational regulators (DKC1, RPS13, miR221, miR222) and screened for DKC1 variants in sporadic pituitary adenomas. Samples were obtained during transsphenoidal surgery from 48 patients with pituitary adenomas: 10 ACTH-, 17 GH-secreting, and 21 nonfunctioning (NFPA). The control group comprised 7 normal pituitaries (NP) obtained during autopsies. Gene expression was assessed by RT-PCR and protein expression by immunohistochemistry. The 15 exons of DKC1 were sequenced. P27 protein underexpression was observed in all adenomas subtypes (p=0.001). CCNE1 mRNA (p=0.01) overexpression, but not protein, was observed in NFPA. No differential gene expression among groups was observed in CDKN1B regulators RPS13 (p=0.23) and DKC1 (p=0.34). The expression of miR221 and miR222 was similar among tumors and NP. Frequent DKC1 variants (SNPs) were found in exon 14 and in the 3'-UTR in similar frequency to NCBI-dsSNP databases. We also observed rare DKC1 variants in 11% of the studied tumor samples, indicating a high prevalence in pituitary adenomas, however, in silico studies failed to indicate deleterious effects. The high frequency of DKC1 variants may influence, in some extent, pituitary tumors development, without clear role in its tumorigenesis. Our data reinforce the P27 underexpression in pituitary adenomas and provide further evidence of the post-translational machinery involvement, although this phenomenon cannot be explained either by mis-expression of P27 translational regulators - DKC1, RPS13, miR221, miR222 - or directly by DKC1 mutations.

  10. High Performance Input/Output Systems for High Performance Computing and Four-Dimensional Data Assimilation

    NASA Technical Reports Server (NTRS)

    Fox, Geoffrey C.; Ou, Chao-Wei

    1997-01-01

    The approach of this task was to apply leading parallel computing research to a number of existing techniques for assimilation, and extract parameters indicating where and how input/output limits computational performance. The following was used for detailed knowledge of the application problems: 1. Developing a parallel input/output system specifically for this application 2. Extracting the important input/output characteristics of data assimilation problems; and 3. Building these characteristics s parameters into our runtime library (Fortran D/High Performance Fortran) for parallel input/output support.

  11. 76 FR 11686 - Registration Requirement for Petitioners Seeking to File H-1B Petitions on Behalf of Aliens...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-03

    ... when the demand for H-1B visas greatly exceeds the available supply. 4. Changes Proposed--Registration.... See 8 CFR 214.2(h)(9)(i)(B). During years of high demand for H-1B workers, the H-1B cap has been... education, or a related or affiliated nonprofit entity; (2) are employed at, or have received an offer...

  12. High performance MEMS micro-gyroscope

    NASA Technical Reports Server (NTRS)

    Bae, S. Y.; Hayworth, K. J.; Yee, K. Y.; Shcheglov, K.; Challoner, A. D.; Wiberg, D. V.

    2002-01-01

    This paper reports on JPL's on-going research into MEMS gyroscopes. This paper will describe the gyroscope's fabrication-methods, a new 8-electrode layout developed to improve performance, and performance statistics of a batch of six gyroscopes recently rate tested.

  13. High Performance Liquid Chromatography/Video Fluorometry. Part I. Instrumentation.

    DTIC Science & Technology

    1981-09-30

    High Performance Liquid Chromatography /Video...PERIOD COVERED High Performance Liquid Chromatography /Video .. / Fluorometry. Part I. Instrumentation. . Interim/ echnicaliepart,. 6. PERFORMING ORG...34Entered SECURITY CLASSIFICATION OF THIS OlAGE (When Data Entered) II1| III I I I I E I II ... .. High Performance Liquid Chromatography

  14. Spectrally high performing quantum cascade lasers

    NASA Astrophysics Data System (ADS)

    Toor, Fatima

    Quantum cascade (QC) lasers are versatile semiconductor light sources that can be engineered to emit light of almost any wavelength in the mid- to far-infrared (IR) and terahertz region from 3 to 300 mum [1-5]. Furthermore QC laser technology in the mid-IR range has great potential for applications in environmental, medical and industrial trace gas sensing [6-10] since several chemical vapors have strong rovibrational frequencies in this range and are uniquely identifiable by their absorption spectra through optical probing of absorption and transmission. Therefore, having a wide range of mid-IR wavelengths in a single QC laser source would greatly increase the specificity of QC laser-based spectroscopic systems, and also make them more compact and field deployable. This thesis presents work on several different approaches to multi-wavelength QC laser sources that take advantage of band-structure engineering and the uni-polar nature of QC lasers. Also, since for chemical sensing, lasers with narrow linewidth are needed, work is presented on a single mode distributed feedback (DFB) QC laser. First, a compact four-wavelength QC laser source, which is based on a 2-by-2 module design, with two waveguides having QC laser stacks for two different emission wavelengths each, one with 7.0 mum/11.2 mum, and the other with 8.7 mum/12.0 mum is presented. This is the first design of a four-wavelength QC laser source with widely different emission wavelengths that uses minimal optics and electronics. Second, since there are still several unknown factors that affect QC laser performance, results on a first ever study conducted to determine the effects of waveguide side-wall roughness on QC laser performance using the two-wavelength waveguides is presented. The results are consistent with Rayleigh scattering effects in the waveguides, with roughness effecting shorter wavelengths more than longer wavelengths. Third, a versatile time-multiplexed multi-wavelength QC laser system that

  15. 18 CFR 1b.7 - Procedure after investigation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Procedure after investigation. 1b.7 Section 1b.7 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.7 Procedure after investigation...

  16. 18 CFR 1b.8 - Requests for Commission investigations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Requests for Commission investigations. 1b.8 Section 1b.8 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.8 Requests for Commission...

  17. 18 CFR 1b.9 - Confidentiality of investigations.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Confidentiality of investigations. 1b.9 Section 1b.9 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION... Freedom of Information Act disclosure are set forth in 18 CFR part 3b and § 1b.20. A request for...

  18. 18 CFR 1b.9 - Confidentiality of investigations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Confidentiality of investigations. 1b.9 Section 1b.9 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION... Freedom of Information Act disclosure are set forth in 18 CFR part 3b and § 1b.20. A request for...

  19. 18 CFR 1b.7 - Procedure after investigation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Procedure after investigation. 1b.7 Section 1b.7 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.7 Procedure after investigation...

  20. 18 CFR 1b.10 - By whom conducted.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false By whom conducted. 1b.10 Section 1b.10 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES RULES RELATING TO INVESTIGATIONS § 1b.10 By whom conducted. Formal...