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Sample records for 200 200c 300

  1. 76 FR 12619 - Airworthiness Directives; The Boeing Company 737-200, -200C, -300, -400, and -500 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-08

    ...-18-06, Amendment 39-13784 (69 FR 54206, September 8, 2004), for certain Model 737-200, -200C, -300... 12866, (2) Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034...), 40113, 44701. Sec. 39.13 2. The FAA amends Sec. 39.13 by removing amendment 39-13784 (69 FR...

  2. 76 FR 72858 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ... (69 FR 23646, April 30, 2004), for all Boeing Model 737-200C series airplanes. That AD requires... decompression of the airplane. On July 23, 2009, we issued AD 2009-16-14, Amendment 39-15987 (74 FR 38901... Since we issued AD 2004-09-09, Amendment 39-13598 (69 FR 23646, April 30, 2004), we have...

  3. The Chemical Interplay of Serpentinization, Methanogenesis, and Carbonate Formation at 200C and 300 bar

    NASA Astrophysics Data System (ADS)

    Jones, L.; Rosenbauer, R.; Oze, C.

    2008-12-01

    Serpentinization (olivine hydrolysis) is the primary process contributing to abiotic generation of elemental hydrogen (H2) in active mid-ocean ridge hydrothermal systems and forearc environments. Carbon dioxide in these types of systems reacts with serpentinization-derived H2 to produce methane (CH4) and with cations in seawater (Ca2+, Mg2+, Fe2+) to precipitate carbonate minerals. Despite a growing body of field and experimental evidence, the reaction pathways, kinetics, and geochemical interplay of serpentinization, methanogenesis and carbonate formation are not fully understood. Here, we evaluated the reaction kinetics of CH4 versus carbonate genesis during serpentinization of average Earth olivines (Fo~90) under pressure-temperature (PT) conditions similar to mid-ocean ridge and forearc environments. Laboratory experiments were preformed at identical T and P conditions (200°C and 300 bar) using flexible gold reaction-cells in hydrothermal autoclaves located in the Water- Rock Interaction Laboratory at the U.S. Geological Survey in Menlo Park, CA. The experiments demonstrate that CH4 production was minimal in CO2-rich fluids and that CH4 generation was enhanced in a CO2-limited experiment producing an end-member CH4 concentration of 44 μM after 860 hours. Equilibrium thermodynamics and reaction rates observed in these experiments demonstrate that carbonate precipitation is favored in CO2-saturated systems and methanogenesis is hindered. These results highlight the competition between methanogenesis and carbonate production in hydrothermal environments leading to the conclusion that the production of CH4 is greater when the concentration of CO2 is undersaturated with respect to carbonate equilibrium.

  4. 76 FR 54399 - Airworthiness Directives; The Boeing Company Model 737-200, -200C, -300, -400, and -500 Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-01

    ... 2010-01-09, Amendment 39-16167 (75 FR 1527, January 12, 2010), for certain Model 737-300, -400, and... a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26...-09, Amendment 39-16167 (75 FR 1527, January 12, 2010), and adding the following new AD: The...

  5. DYNAMIC LOADING OF TEFLON AT 200?C

    SciTech Connect

    Urtiew, P A; Forbes, J W; Tarver, C M; Vandersall, K S; Garcia, F

    2007-06-13

    Dynamic loading experiments were performed on inert Teflon (Polytetrafluoroethylene) samples, initially heated to the temperature of 200 C, to test its behavior under these conditions for its use in other heated experiments. Tests were performed in the 100 mm diameter bore propellant driven gas gun with piezo-resistive manganin pressure gauges imbedded into the samples to measure loading pressures. Experimental data provided new information on the shock velocity - particle velocity relationship for the heated material and showed no adverse effect of temperature on the insulating properties of the material.

  6. Operational characteristics of a 200 C LC parallel resonant circuit

    NASA Technical Reports Server (NTRS)

    Baumann, Eric D.; Hammoud, Ahmad N.

    1995-01-01

    Research efforts are currently underway at the NASA Lewis Research Center to design and demonstrate an inverter capable of operating with a baseplate temperature of 200 C. In support of this project, various electrical components including capacitors, inductors, transformers, cables, and semiconductor switches are being developed or evaluated for integration into the inverter. In this work, a parallel LC resonant circuit was constructed and evaluated under simultaneous electrical and thermal stressing. The tests were performed in the temperature range of 25 to 200 C with an applied voltage of up to 90 V, 20 kHz. The individual components were comprised of high temperature film capacitors and powder core inductors developed in-house. The circuit was characterized in terms of the component currents and case temperatures as well as frequency of resonance as a function of applied bias and temperature. The results obtained, which have indicated good functional stability up to 200 C, are presented and discussed.

  7. miR-200c Contributes to Injury From Transient Focal Cerebral Ischemia by Targeting Reelin

    PubMed Central

    Stary, Creed M.; Xu, Lijun; Sun, Xiaoyun; Ouyang, Yi-Bing; White, Robin E.; Leong, Jason; Li, John; Xiong, Xiaoxing; Giffard, Rona G.

    2015-01-01

    Background and Purpose miR-200c increases rapidly in the brain following transient cerebral ischemia but its role in post-stroke brain injury is unclear. Reelin, a regulator of neuronal migration and synaptogenesis, is a predicted target of miR-200c. We hypothesized that miR-200c contributes to injury from transient cerebral ischemia by targeting reelin. Methods Brain infarct volume, neurological score and levels of miR-200c, reelin mRNA and reelin protein were assessed in mice subjected to 1 hr of middle cerebral artery occlusion (MCAO) with/without intracerebroventricular infusion of miR-200c antagomir, mimic or mismatch control. Direct targeting of reelin by miR-200c was assessed in vitro by dual luciferase assay and immunoblot. Results Pre-treatment with miR-200c antagomir decreased post-MCAO brain levels of miR-200c, resulting in a significant reduction in infarct volume and neurologic deficit. Changes in brain levels of miR-200c inversely correlated with reelin protein expression. Direct targeting of the Reln 3’UTR by miR-200c was verified with dual luciferase assay. Inhibition of miR-200c resulted in an increase in cell survival subsequent to in vitro oxidative injury. This effect was blocked by knockdown of reelin mRNA, while application of reelin protein afforded protection. Conclusions These findings suggest that the post-stroke increase in miR-200c contributes to brain cell death by inhibiting reelin expression, and that reducing post-stroke miR-200c is a potential target to mitigate stroke induced brain injury. PMID:25604249

  8. miR-200c dampens cancer cell migration via regulation of protein kinase A subunits.

    PubMed

    Sigloch, Florian Christoph; Burk, Ulrike Christina; Biniossek, Martin Lothar; Brabletz, Thomas; Schilling, Oliver

    2015-09-15

    Expression of miR-200c is a molecular switch to determine cellular fate towards a mesenchymal or epithelial phenotype. miR-200c suppresses the early steps of tumor progression by preventing epithelial-mesenchymal transition (EMT) and intravasation of tumor cells. Unraveling the underlying molecular mechanisms might pinpoint to novel therapeutic options. To better understand these mechanisms it is crucial to identify targets of miR-200c. Here, we employ a combination of quantitative proteomic and bioinformatic strategies to identify novel miR-200c targets. We identify and confirm two subunits of the central cellular kinase protein kinase A (PKA), namely PRKAR1A and PRKACB, to be directly regulated by miR-200c. Notably, siRNA-mediated downregulation of both proteins phenocopies the migratory behavior of breast cancer cells after miR-200c overexpression. Patient data from publicly accessible databases supports a miR-200c-PKA axis. Thus, our study identifies the PKA heteroprotein as an important mediator of miR-200c induced repression of migration in breast cancer cells. By bioinformatics, we define a miRNA target cluster consisting of PRKAR1A, PRKAR2B, PRKACB, and COF2, which is targeted by a group of 14 miRNAs. PMID:26203557

  9. miR-200c dampens cancer cell migration via regulation of protein kinase a subunits

    PubMed Central

    Biniossek, Martin Lothar; Brabletz, Thomas; Schilling, Oliver

    2015-01-01

    Expression of miR-200c is a molecular switch to determine cellular fate towards a mesenchymal or epithelial phenotype. miR-200c suppresses the early steps of tumor progression by preventing epithelial-mesenchymal transition (EMT) and intravasation of tumor cells. Unraveling the underlying molecular mechanisms might pinpoint to novel therapeutic options. To better understand these mechanisms it is crucial to identify targets of miR-200c. Here, we employ a combination of quantitative proteomic and bioinformatic strategies to identify novel miR-200c targets. We identify and confirm two subunits of the central cellular kinase protein kinase A (PKA), namely PRKAR1A and PRKACB, to be directly regulated by miR-200c. Notably, siRNA-mediated downregulation of both proteins phenocopies the migratory behavior of breast cancer cells after miR-200c overexpression. Patient data from publicly accessible databases supports a miR-200c-PKA axis. Thus, our study identifies the PKA heteroprotein as an important mediator of miR-200c induced repression of migration in breast cancer cells. By bioinformatics, we define a miRNA target cluster consisting of PRKAR1A, PRKAR2B, PRKACB, and COF2, which is targeted by a group of 14 miRNAs. PMID:26203557

  10. MiR-200c promotes bladder cancer cell migration and invasion by directly targeting RECK

    PubMed Central

    Cheng, Yidong; Zhang, Xiaolei; Li, Peng; Yang, Chengdi; Tang, Jinyuan; Deng, Xiaheng; Yang, Xiao; Tao, Jun; Lu, Qiang; Li, Pengchao

    2016-01-01

    Background Increasing evidence suggests that the dysregulation of certain microRNAs plays an important role in tumorigenesis and metastasis. MiR-200c exhibits a disordered expression in many tumors and presents dual roles in bladder cancer (BC). Therefore, the definite role of miR-200c in BC needs to be investigated further. Materials and methods Quantitative reverse transcription polymerase chain reaction was used to assess miR-200c expression. Cell invasion and migration were evaluated using wound healing and transwell assays. The luciferase reporter assay was used to identify the direct target of miR-200c. The expression of reversion-inducing cysteine-rich protein with kazal motifs (RECK) in BC tissues and adjacent nontumor tissues, as well as in BC cell lines, was detected through quantitative reverse transcription polymerase chain reaction, Western blot assay, and immunohistochemistry. Results The miR-200c expression was significantly upregulated in the BC tissues compared with the adjacent nontumor tissues. The downregulation of miR-200c significantly inhibited cell migration and invasion in the BC cell lines. The luciferase reporter assay showed that RECK was a direct target of miR-200c. The knockdown of RECK in the BC cell lines treated with anti-miR-200c elevated the previously attenuated cell migration and invasion. Conclusion Our findings indicated that miR-200c functions as oncogenes in BC and may provide a novel therapeutic strategy for the treatment of BC. PMID:27574450

  11. Development of a 1 kW, 200 C Mapham Inventor

    NASA Technical Reports Server (NTRS)

    Hammoud, Ahmad; Gerber, Scott; Bauman, Eric; Overton, Eric; Myers, Ira; Bercaw, Robert

    1995-01-01

    Electronic systems and components are often exposed to high temperature environment in space-based applications, nuclear power facilities, and geothermal energy extraction fields. A key requirement for these systems is, therefore, to withstand the high temperature exposure while maintaining efficient and reliable operation. Efforts were taken to design and develop a high temperature power inverter capable of 200 C operation. A 1 kW, 20 kHz Mapham inverter was designed and evaluated as a function of temperature at different load levels. The inverter system, excluding its input, control, and logic circuits, was characterized at temperatures from ambient to 200 C at 0%, 50%, and 100% resistive loading. With an applied input voltage of 75 VDC, the inverter produced an output of 250 VAC. The results obtained, which indicate good operational characteristics of the inverter up to 200 C, are presented and discussed.

  12. miR-200c: a versatile watchdog in cancer progression, EMT, and drug resistance.

    PubMed

    Mutlu, Merve; Raza, Umar; Saatci, Özge; Eyüpoğlu, Erol; Yurdusev, Emre; Şahin, Özgür

    2016-06-01

    MicroRNAs (miRNAs) are 20-22-nucleotide small endogenous non-coding RNAs which regulate gene expression at post-transcriptional level. In the last two decades, identification of almost 2600 miRNAs in human and their potential to be modulated opened a new avenue to target almost all hallmarks of cancer. miRNAs have been classified as tumor suppressors or oncogenes depending on the phenotype they induce, the targets they modulate, and the tissue where they function. miR-200c, an illustrious tumor suppressor, is one of the highly studied miRNAs in terms of development, stemness, proliferation, epithelial-mesenchymal transition (EMT), therapy resistance, and metastasis. In this review, we first focus on the regulation of miR-200c expression and its role in regulating EMT in a ZEB1/E-cadherin axis-dependent and ZEB1/E-cadherin axis-independent manner. We then describe the role of miR-200c in therapy resistance in terms of multidrug resistance, chemoresistance, targeted therapy resistance, and radiotherapy resistance in various cancer types. We highlight the importance of miR-200c at the intersection of EMT and chemoresistance. Furthermore, we show how miR-200c coordinates several important signaling cascades such as TGF-β signaling, PI3K/Akt signaling, Notch signaling, VEGF signaling, and NF-κB signaling. Finally, we discuss miR-200c as a potential prognostic/diagnostic biomarker in several diseases, but mainly focusing on cancer and its potential application in future therapeutics. PMID:27094812

  13. Inhibition of miR-200c Restores Endothelial Function in Diabetic Mice Through Suppression of COX-2.

    PubMed

    Zhang, Huina; Liu, Jian; Qu, Dan; Wang, Li; Luo, Jiang-Yun; Lau, Chi Wai; Liu, Pingsheng; Gao, Zhen; Tipoe, George L; Lee, Hung Kay; Ng, Chi Fai; Ma, Ronald Ching Wan; Yao, Xiaoqiang; Huang, Yu

    2016-05-01

    Endothelial dysfunction plays a crucial role in the development of diabetic vasculopathy. Our initial quantitative PCR results showed an increased miR-200c expression in arteries from diabetic mice and patients with diabetes. However, whether miR-200c is involved in diabetic endothelial dysfunction is unknown. Overexpression of miR-200c impaired endothelium-dependent relaxations (EDRs) in nondiabetic mouse aortas, whereas suppression of miR-200c by anti-miR-200c enhanced EDRs in diabetic db/db mice. miR-200c suppressed ZEB1 expression, and ZEB1 overexpression ameliorated endothelial dysfunction induced by miR-200c or associated with diabetes. More importantly, overexpression of anti-miR-200c or ZEB1 in vivo attenuated miR-200c expression and improved EDRs in db/db mice. Mechanistic study with the use of COX-2(-/-) mice revealed that COX-2 mediated miR-200c-induced endothelial dysfunction and that miR-200c upregulated COX-2 expression in endothelial cells through suppression of ZEB1 and increased production of prostaglandin E2, which also reduced EDR. This study demonstrates for the first time to our knowledge that miR-200c is a new mediator of diabetic endothelial dysfunction and inhibition of miR-200c rescues EDRs in diabetic mice. These new findings suggest the potential usefulness of miR-200c as the target for drug intervention against diabetic vascular complications. PMID:26822089

  14. Deutsch 100, 200, 300.

    ERIC Educational Resources Information Center

    Manitoba Dept. of Education, Winnipeg.

    Manitoba's curriculum guide for German 100, 200, and 300 is designed to (1) make teachers of these courses aware of the official policy concerning the nature, goals, and objectives of the courses and approved teaching materials, and (2) provide instructional guidelines. The guide provides information about three sets of teaching materials from…

  15. Early antihepatitis C virus response with second-generation C200/C22 ELISA.

    PubMed

    van der Poel, C L; Bresters, D; Reesink, H W; Plaisier, A A; Schaasberg, W; Leentvaar-Kuypers, A; Choo, Q L; Quan, S; Polito, A; Houghton, M

    1992-01-01

    Detection of early antibody to hepatitis C virus (HCV) by a new second-generation C200/C22 anti-HCV enzyme-linked immunosorbent assay (ELISA) and a four-antigen recombinant immunoblot assay (4-RIBA) was compared with the first-generation anti-HCV C100 ELISA using sequential serum samples of 9 recipients who were infected with HCV, as detected by polymerase chain reaction after transfusion of blood products. Within 26 weeks after transfusion, 9/9 (100%) recipients seroconverted with C200/22 ELISA, and 6/9 (67%) seroconverted with C100 ELISA. Compared with C100 ELISA, C200/C22 ELISA seroconversion occurred simultaneously in 3 cases, 5-6 weeks earlier in 3 other cases, and 20 weeks earlier in 1 case. Seven of 9 (78%) recipients became positive, and 2/9 (22%) became indeterminate with 4-RIBA. In 8 cases with clinical posttransfusion hepatitis non-A, non-B (PTH-NANB), anti-HCV C200/C22 ELISA seroconversion occurred 2-17 (mean 6) weeks after the onset of hepatitis. In 6 cases of PTH-NANB, anti-HCV C100 ELISA seroconversion occurred 2-26 (mean 9) weeks after the onset of hepatitis. It is concluded that the second-generation C200/C22 ELISA is more sensitive than the C100 ELISA for the detection of antibody during early HCV infection. Indeterminate 4-RIBA results are found in the early phase of HCV infection. PMID:1322611

  16. Regulation of miR-200c by nuclear receptors PPAR{alpha}, LRH-1 and SHP

    SciTech Connect

    Zhang, Yuxia; Yang, Zhihong; Whitby, Richard; Wang, Li

    2011-12-09

    Highlights: Black-Right-Pointing-Pointer Knockdown of PPAR{alpha} and LRH-1 abolishes miR-200c inhibition of HCC cell migration. Black-Right-Pointing-Pointer SHP represses miR-200c expression via inhibition of the activity of PPAR{alpha} and LRH-1. Black-Right-Pointing-Pointer RJW100 exhibits strong ability to downregulate ZEB1 and ZEB2 proteins. -- Abstract: We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPAR{alpha} and LRH-1 siRNAs. The expression of miR-200c was decreased by PPAR{alpha}/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins. Co-expression of PPAR{alpha} and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter. This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors.

  17. Prognostic Role of MicroRNA-200c-141 Cluster in Various Human Solid Malignant Neoplasms

    PubMed Central

    Li, Xiao-yang; Li, Hui; Bu, Jie; Xiong, Liang; Guo, Hong-bin; Liu, Li-hong; Xiao, Tao

    2015-01-01

    The miR-200 family has emerged recently as a noticeable marker for predicting cancer prognosis and tumor progression. We aimed to review the evidence of miR-200c-141 genomic cluster as prognostic biomarkers in cancers. The results suggested that high level of miR-200c had no significant impact on OS (HR = 1.14 [0.77–1.69], P = 0.501) and DFS/PFS (HR = 0.72 [0.45–1.14], P = 0.161). Stratified analyses revealed that high miR-200c expression was significantly related to poor OS in serum/plasma (HR = 2.12 [1.62–2.77], P = 0.000) but not in tissues (HR = 0.89 [0.58–1.37], P = 0.599). High miR-200c expression was significantly associated with favorable DFS/PFS in tissues (HR = 0.56 [0.43–0.73], P = 0.000) but worse DFS/PFS in serum/plasma (HR = 1.90 [1.08–3.36], P = 0.027). For miR-141, we found that high miR-141 expression predicted no significant impact on OS (HR = 1.18 [0.74–1.88], P = 0.482) but poor DFS/PFS (HR = 1.11 [1.04–1.20], P = 0.003). Similarly, subgroup analyses showed that high miR-141 expression predicted poor OS in serum/plasma (HR = 4.34 [2.30–8.21], P = 0.000) but not in tissues (HR = 1.00 [0.92–1.09], P = 0.093). High miR-141 expression was significantly associated with worse DFS/PFS in tissues (HR = 1.12 [1.04–1.20], P = 0.002) but not in serum/plasma (HR = 0.90 [0.44–1.83], P = 0.771). Our findings indicated that, compared to their tissue counterparts, the expression level of miR-200c and miR-141 in peripheral blood may be more effective for monitoring cancer prognosis. High miR-141 expression was better at predicting tumor progression than survival for malignant tumors. PMID:26556949

  18. The Pitx2:miR-200c/141:noggin pathway regulates Bmp signaling and ameloblast differentiation

    PubMed Central

    Cao, Huojun; Jheon, Andrew; Li, Xiao; Sun, Zhao; Wang, Jianbo; Florez, Sergio; Zhang, Zichao; McManus, Michael T.; Klein, Ophir D.; Amendt, Brad A.

    2013-01-01

    The mouse incisor is a remarkable tooth that grows throughout the animal’s lifetime. This continuous renewal is fueled by adult epithelial stem cells that give rise to ameloblasts, which generate enamel, and little is known about the function of microRNAs in this process. Here, we describe the role of a novel Pitx2:miR-200c/141:noggin regulatory pathway in dental epithelial cell differentiation. miR-200c repressed noggin, an antagonist of Bmp signaling. Pitx2 expression caused an upregulation of miR-200c and chromatin immunoprecipitation assays revealed endogenous Pitx2 binding to the miR-200c/141 promoter. A positive-feedback loop was discovered between miR-200c and Bmp signaling. miR-200c/141 induced expression of E-cadherin and the dental epithelial cell differentiation marker amelogenin. In addition, miR-203 expression was activated by endogenous Pitx2 and targeted the Bmp antagonist Bmper to further regulate Bmp signaling. miR-200c/141 knockout mice showed defects in enamel formation, with decreased E-cadherin and amelogenin expression and increased noggin expression. Our in vivo and in vitro studies reveal a multistep transcriptional program involving the Pitx2:miR-200c/141:noggin regulatory pathway that is important in epithelial cell differentiation and tooth development. PMID:23863486

  19. miR-200c regulates FGFR-dependent epithelial proliferation via Vldlr during submandibular gland branching morphogenesis

    PubMed Central

    Rebustini, Ivan T.; Hayashi, Toru; Reynolds, Andrew D.; Dillard, Melvin L.; Carpenter, Ellen M.; Hoffman, Matthew P.

    2012-01-01

    The regulation of epithelial proliferation during organ morphogenesis is crucial for normal development, as dysregulation is associated with tumor formation. Non-coding microRNAs (miRNAs), such as miR-200c, are post-transcriptional regulators of genes involved in cancer. However, the role of miR-200c during normal development is unknown. We screened miRNAs expressed in the mouse developing submandibular gland (SMG) and found that miR-200c accumulates in the epithelial end buds. Using both loss- and gain-of-function, we demonstrated that miR-200c reduces epithelial proliferation during SMG morphogenesis. To identify the mechanism, we predicted miR-200c target genes and confirmed their expression during SMG development. We discovered that miR-200c targets the very low density lipoprotein receptor (Vldlr) and its ligand reelin, which unexpectedly regulate FGFR-dependent epithelial proliferation. Thus, we demonstrate that miR-200c influences FGFR-mediated epithelial proliferation during branching morphogenesis via a Vldlr-dependent mechanism. miR-200c and Vldlr may be novel targets for controlling epithelial morphogenesis during glandular repair or regeneration. PMID:22115756

  20. Switching Characteristics of a 4H-SiC Based Bipolar Junction Transistor to 200 C

    NASA Technical Reports Server (NTRS)

    Niedra, Janis M.

    2006-01-01

    Static curves and resistive load switching characteristics of a 600 V, 4 A rated, SiC-based NPN bipolar power transistor (BJT) were observed at selected temperatures from room to 200 C. All testing was done in a pulse mode at low duty cycle (approx.0.1 percent). Turn-on was driven by an adjustable base current pulse and turn-off was accelerated by a negative base voltage pulse of 7 V. These base drive signals were implemented by 850 V, gated power pulsers, having rise-times of roughly 10 ns, or less. Base charge sweep-out with a 7 V negative pulse did not produce the large reverse base current pulse seen in a comparably rated Si-based BJT. This may be due to a very low charge storage time. The decay of the collector current was more linear than its exponential-like rise. Switching observations were done at base drive currents (I(sub B)) up to 400 mA and collector currents (I(sub C)) up to 4 A, using a 100 Omega non-inductive load. At I(sub B) = 400 mA and I(sub C) = 4 A, turn-on times typically varied from 80 to 94 ns, over temperatures from 23 to 200 C. As expected, lowering the base drive greatly extended the turn-on time. Similarly, decreasing the load current to I(sub C) = 1 A with I(sub B) = 400 mA produced turn-on times as short as 34 ns. Over the 23 to 200 C range, with I(sub B) = 400 mA and I(sub C) = 4 A, turn-off times were in the range of 72 to 84 ns with the 7 V sweep-out.

  1. A 200 C Universal Gate Driver Integrated Circuit for Extreme Environment Applications

    SciTech Connect

    Tolbert, Leon M; Huque, Mohammad A; Islam, Syed K; Blalock, Benjamin J

    2012-01-01

    High-temperature power converters (dc-dc, dc-ac, etc.) have enormous potential in extreme environment applications, including automotive, aerospace, geothermal, nuclear, and well logging. For successful realization of such high-temperature power conversion modules, the associated control electronics also need to perform at high temperature. This paper presents a silicon-on-insulator (SOI) based high-temperature gate driver integrated circuit (IC) incorporating an on-chip low-power temperature sensor and demonstrating an improved peak output current drive over our previously reported work. This driver IC has been primarily designed for automotive applications, where the underhood temperature can reach 200 C. This new gate driver prototype has been designed and implemented in a 0.8 {micro}m, 2-poly, and 3-metal bipolar CMOS-DMOS (Double-Diffused Metal-Oxide Semiconductor) on SOI process and has been successfully tested for up to 200 C ambient temperature driving a SiC MOSFET and a SiC normally-ON JFET. The salient feature of the proposed universal gate driver is its ability to drive power switches over a wide range of gate turn-ON voltages such as MOSFET (0 to 20 V), normally-OFF JFET (-7 to 3 V), and normally-ON JFET (-20 to 0 V). The measured peak output current capability of the driver is around 5 A and is thus capable of driving several power switches connected in parallel. An ultralow-power on-chip temperature supervisory circuit has also been integrated into the die to safeguard the driver circuit against excessive die temperature ({ge}220 C). This approach utilizes increased diode leakage current at higher temperature to monitor the die temperature. The power consumption of the proposed temperature sensor circuit is below 10 {micro}W for operating temperature up to 200 C.

  2. MicroRNA-200c Represses IL-6, IL-8, and CCL-5 Expression and Enhances Osteogenic Differentiation

    PubMed Central

    Sharp, Thad; Khorsand, Behnoush; Fischer, Carol; Eliason, Steven; Salem, Ali; Akkouch, Adil; Brogden, Kim; Amendt, Brad A.

    2016-01-01

    MicroRNAs (miRs) regulate inflammation and BMP antagonists, thus they have potential uses as therapeutic reagents. However, the molecular function of miR-200c in modulating proinflammatory and bone metabolic mediators and osteogenic differentiation is not known. After miR-200c was transduced into a human embryonic palatal mesenchyme (HEPM) (a cell line of preosteoblasts), using lentiviral vectors, the resulting miR-200c overexpression increased osteogenic differentiation biomarkers, including osteocalcin (OCN) transcripts and calcium content. miR-200c expression also down-regulated interleukin (IL)-6, IL-8, and chemokine (C-C motif) ligand (CCL)-5 under lipopolysaccharide (LPS) stimulation and increased osteoprotegerin (OPG) in these cells. miR-200c directly regulates the expression of IL-6, IL-8 and CCL-5 transcripts by binding to their 3’UTRs. A plasmid-based miR-200c inhibitor effectively reduces their binding activities. Additionally, miR-200c delivered using polyethylenimine (PEI) nanoparticles effectively inhibits IL-6, IL-8 and CCL-5 in primary human periodontal ligament fibroblasts and increases the biomarkers of osteogenic differentiation in human bone marrow mesenchymal stem cells (MSCs), including calcium content, ALP, and Runx2. These data demonstrate that miR-200c represses IL-6, IL-8 and CCL-5 and improves osteogenic differentiation. miR-200c may potentially be used as an effective means to prevent periodontitis-associated bone loss by arresting inflammation and osteoclastogenesis and enhancing bone regeneration. PMID:27529418

  3. MicroRNA-200c Represses IL-6, IL-8, and CCL-5 Expression and Enhances Osteogenic Differentiation.

    PubMed

    Hong, Liu; Sharp, Thad; Khorsand, Behnoush; Fischer, Carol; Eliason, Steven; Salem, Ali; Akkouch, Adil; Brogden, Kim; Amendt, Brad A

    2016-01-01

    MicroRNAs (miRs) regulate inflammation and BMP antagonists, thus they have potential uses as therapeutic reagents. However, the molecular function of miR-200c in modulating proinflammatory and bone metabolic mediators and osteogenic differentiation is not known. After miR-200c was transduced into a human embryonic palatal mesenchyme (HEPM) (a cell line of preosteoblasts), using lentiviral vectors, the resulting miR-200c overexpression increased osteogenic differentiation biomarkers, including osteocalcin (OCN) transcripts and calcium content. miR-200c expression also down-regulated interleukin (IL)-6, IL-8, and chemokine (C-C motif) ligand (CCL)-5 under lipopolysaccharide (LPS) stimulation and increased osteoprotegerin (OPG) in these cells. miR-200c directly regulates the expression of IL-6, IL-8 and CCL-5 transcripts by binding to their 3'UTRs. A plasmid-based miR-200c inhibitor effectively reduces their binding activities. Additionally, miR-200c delivered using polyethylenimine (PEI) nanoparticles effectively inhibits IL-6, IL-8 and CCL-5 in primary human periodontal ligament fibroblasts and increases the biomarkers of osteogenic differentiation in human bone marrow mesenchymal stem cells (MSCs), including calcium content, ALP, and Runx2. These data demonstrate that miR-200c represses IL-6, IL-8 and CCL-5 and improves osteogenic differentiation. miR-200c may potentially be used as an effective means to prevent periodontitis-associated bone loss by arresting inflammation and osteoclastogenesis and enhancing bone regeneration. PMID:27529418

  4. Static and Switching Characteristics of a 4H-SiC Based BJT to 200 C

    NASA Technical Reports Server (NTRS)

    Niedra, Janis M.; Schwarze, Gene E.

    2006-01-01

    Static curves and resistive load switching characteristics of a 600 V, 4 A rated, SiC-based NPN bipolar power transistor (BJT) were observed at selected temperatures from 23 to 200 C. All testing was done in a pulse mode at low duty cycle (approx. 0.1 percent). Turn-on was driven by an adjustable base current pulse and turn-off was accelerated by a negative base voltage pulse of either 7 or 0.6 V. Switching observations were done at base drive currents (I(sub B)) up to 400 mA and collector currents (I(sub C)) up to 4 A, using a 100 OMEGA non-inductive load. At I(sub B) = 400 mA and I(sub C) = 4 A, turn-on times typically varied from 81 to 97 ns, over temperatures from 23 to 200 C. As expected, lowering the base drive greatly extended the turn-on time. Similarly, decreasing the load current to I(sub C) = 1 A with I(sub B) = 400 mA produced turn-on times as short as 30 ns. Over the 23 to 200 C range, with I(sub B) = 400 mA and I(sub C) = 4 A, turn-off times were in the range of 61 to 77 ns with the 7 V sweep-out and 130 to 150 ns with the 0.6 V sweep-out. At a fixed temperature and I(sub C), the turn-off time decreased slightly with decreasing I(sub B), for I(sub B) sufficient to still provide full turn-on. The ratio of conduction to switching losses is estimated, based on the observed I(sub C) transition times and static curves. An estimate at 200 kHz and a 50 percent duty cycle shows that under practicable conditions the two losses can be comparable. Hence the evidence obtained does not support the occasionally voiced concern of necessarily unacceptably high conduction losses in SiC-based BJTs.

  5. Nuclear Melt Glass Dissolution and Secondary Mineral Precipitation at 40 to 200C

    SciTech Connect

    Zavarin, M; Roberts, S; Viani, B; Pawloski, G; Rose, T

    2004-06-14

    Most long-lived radionuclides associated with an underground nuclear test are initially incorporated into melt glass and become part of the hydrologic source term (HST) only upon their release via glass dissolution (Pawloski et al., 2001). As the melt glass dissolves, secondary minerals precipitate. The types of secondary minerals that precipitate influence the water chemistry in and around the melt glass. The secondary minerals also provide a sorption sink to the released radionuclides. The changing water chemistry affects the rate of glass dissolution; it also affects the sorption behavior of the released radionuclides. This complex nature of glass dissolution and its important role in defining the HST requires a thorough understanding of glass dissolution and secondary mineral precipitation. The identity of secondary minerals formed at temperatures from 40 to 200 C are evaluated in this report to assist in that understanding.

  6. miR-200c Inhibits Melanoma Progression and Drug Resistance through Down-Regulation of Bmi-1

    PubMed Central

    Liu, Shujing; Tetzlaff, Michael T.; Cui, Rutao; Xu, Xiaowei

    2013-01-01

    MicroRNAs (miRNAs) are short noncoding RNAs that play crucial roles in tumorigenesis and tumor progression. Melanoma is the most aggressive skin cancer that is resistant or rapidly develops resistance to a variety of chemotherapeutic agents. The role of miRNAs in melanoma progression and drug resistance has not been well studied. Herein, we demonstrate that miR-200c is down-regulated in melanomas (primary and metastatic) compared with melanocytic nevi. Overexpression of miR-200c in melanoma cells resulted in significantly decreased cell proliferation and migratory capacity as well as drug resistance. miR-200c overexpression resulted in significant down-regulation of BMI-1, ABCG2, ABCG5, and MDR1 expression and in a concomitant increase in E-cadherin levels. Knockdown of BMI-1 showed similar effects as miR-200c overexpression in melanoma cells. In addition, miR-200c overexpression significantly inhibited melanoma xenograft growth and metastasis in vivo, and this correlated with diminished expression of BMI-1 and reduced levels of E-cadherin in these tumors. The effects of miR-200c on melanoma cell proliferation and migratory capacity and on self-renewal were rescued by overexpression of Bmi-1, and the reversal of these phenotypes correlated with a reduction in E-cadherin expression and increased levels of ABCG2, ABCG5, and MDR1. Taken together, these findings demonstrate a key role for miR-200c in melanoma progression and drug resistance. These results suggest that miR-200c may represent a critical target for increasing melanoma sensitivity to clinical therapies. PMID:22982443

  7. MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis

    PubMed Central

    Hur, Keun; Toiyama, Yuji; Takahashi, Masanobu; Balaguer, Francesc; Nagasaka, Takeshi; Koike, Junichi; Hemmi, Hiromichi; Koi, Minoru; Boland, C Richard; Goel, Ajay

    2013-01-01

    Objective Distant metastasis is the major cause of cancer-related death in patients with colorectal cancer (CRC). Although the microRNA-200 (miR-200) family is a crucial inhibitor of epithelial-to-mesenchymal transition (EMT) in human cancer, the role of miR-200 members in the pathogenesis of metastatic CRC has not been investigated. Design Fifty-four pairs of primary CRC and corresponding matched liver metastasis tissue specimens were analysed for expression and methylation status of the miR-200 family members. Functional analysis of miR-200c overexpression was investigated in CRC cell lines, and cells were analysed for proliferation, invasion and migration. Expression of several miR-200c target genes (ZEB1, ETS1 and FLT1) and EMT markers (E-cadherin and vimentin) in CRC cell lines and tissue specimens was validated. Results Liver metastasis tissues showed higher expression of miR-200c (primary CRC=1.31 vs. liver metastasis=1.59; p=0.0014) and miR-141 (primary CRC=0.14 vs. liver metastasis=0.17; p=0.0234) than did primary CRCs, which was significantly associated with hypomethylation of the promoter region of these miRNAs (primary CRC=61.2% vs. liver metastasis=46.7%; p<0.0001). The invasive front in primary CRC tissues revealed low miR-200c expression by in situ hybridization analysis. Transfection of miR-200c precursors resulted in enhanced cell proliferation but reduced invasion and migration behaviours in CRC cell lines. Overexpression of miR-200c in CRC cell lines caused reduced expression of putative gene targets, and resulted in increased E-cadherin and reduced vimentin expression. The associations between miR-200c, target genes and EMT markers were validated in primary CRCs and matching liver metastasis tissues. Conclusions miR-200c plays an important role in mediating EMT and metastatic behaviour in the colon. Its expression is epigenetically regulated, and miR-200c may serve as a potential diagnostic marker and therapeutic target for patients with CRC

  8. Regulation of miR-200c/141 expression by intergenic DNA-looping and transcriptional read-through.

    PubMed

    Batista, Luciana; Bourachot, Brigitte; Mateescu, Bogdan; Reyal, Fabien; Mechta-Grigoriou, Fatima

    2016-01-01

    The miR-200 family members have been implicated in stress responses and ovarian tumorigenesis. Here, we find that miR-200c/141 transcription is intimately linked to the transcription of the proximal upstream gene PTPN6 (SHP1) in all physiological conditions tested. PTPN6 and miR-200c/141 are transcriptionally co-regulated by two complementary mechanisms. First, a bypass of the regular PTPN6 polyadenylation signal allows the transcription of the downstream miR-200c/141. Second, the promoters of the PTPN6 and miR-200c/141 transcription units physically interact through a 3-dimensional DNA loop and exhibit similar epigenetic regulation. Our findings highlight that transcription of intergenic miRNAs is a novel outcome of transcriptional read-through and reveal a yet unexplored type of DNA loop associating two closely located promoters. These mechanisms have significant relevance in ovarian cancers and stress response, pathophysiological conditions in which miR-200c/141 exert key functions. PMID:26725650

  9. miR-200c and phospho-AKT as prognostic factors and mediators of osteosarcoma progression and lung metastasis.

    PubMed

    Berlanga, Pablo; Muñoz, Lisandra; Piqueras, Marta; Sirerol, J Antoni; Sánchez-Izquierdo, María Dolors; Hervás, David; Hernández, Miguel; Llavador, Margarita; Machado, Isidro; Llombart-Bosch, Antonio; Cañete, Adela; Castel, Victoria; Font de Mora, Jaime

    2016-08-01

    Lung metastasis is the major cause of death in osteosarcoma patients. However, molecular mechanisms underlying this metastasis remain poorly understood. To identify key molecules related with pulmonary metastasis of pediatric osteosarcomas, we analyzed high-throughput miRNA expression in a cohort of 11 primary tumors and 15 lung metastases. Results were further validated with an independent cohort of 10 primary tumors and 6 metastases. In parallel, we performed immunohistochemical analysis of activated signaling pathways in 36 primary osteosarcomas. Only phospho-AKT associated with lower overall survival in primary tumors, supporting its role in osteosarcoma progression. CTNNB1 expression also associated with lower overall survival but was not strong enough to be considered an independent variable. Interestingly, miR-200c was overexpressed in lung metastases, implicating an inhibitory feed-back loop to PI3K-AKT. Moreover, transfection of miR200c-mimic in U2-OS cells reduced phospho-AKT levels but increased cellular migration and proliferation. Notably, miR-200c expression strongly correlated with miR-141 and with the osteogenic inhibitor miR-375, all implicated in epithelial to mesenchymal transition. These findings contrast epithelial tumors where reduced miR-200c expression promotes metastasis. Indeed, we noted that osteosarcoma cells in the lung also expressed the epithelial marker CDH1, revealing a change in their mesenchymal phenotype. We propose that miR-200c upregulation occurs late in osteosarcoma progression to provide cells with an epithelial phenotype that facilitates their integration in the metastatic lung niche. Thus, our findings identify phospho-AKT in the primary tumor and miR-200c later during tumor progression as prognostic molecules and potential therapeutic targets to prevent progression and metastasis of pediatric osteosarcomas. PMID:27155790

  10. MiR-200b/200c/429 subfamily negatively regulates Rho/ROCK signaling pathway to suppress hepatocellular carcinoma metastasis.

    PubMed

    Wong, Chun-Ming; Wei, Lai; Au, Sandy Leung-Kuen; Fan, Dorothy Ngo-Yin; Zhou, Yuan; Tsang, Felice Ho-Ching; Law, Cheuk-Ting; Lee, Joyce Man-Fong; He, Xianghuo; Shi, Jue; Wong, Carmen Chak-Lui; Ng, Irene Oi-Lin

    2015-05-30

    MiR-200 family is an important regulator of epithelial-mesenchymal transition and has been implicated in human carcinogenesis. However, their expression and functions in human cancers remain controversial. In the work presented here, we showed that miR-200 family members were frequently down-regulated in hepatocellular carcinoma (HCC). Although all five members of miR-200 family inhibited ZEB1/2 expression in HCC cell lines, we showed that overexpression only of the miR-200b/200c/429 subfamily, but not the miR-200a/141 subfamily, resulted in impeded HCC cell migration. Further investigations led to the identification of RhoA and ROCK2 as specific down-stream targets of the miR-200b/200c/429 subfamily. We demonstrated that the miR-200b/200c/429 subfamily inhibited HCC cell migration through modulating Rho/ROCK mediated cell cytoskeletal reorganization and cell-substratum adhesion. Re-expression of miR-200b significantly suppressed lung metastasis of HCC cells in an orthotopic liver implantation model in vivo. In conclusion, our findings identified the miR-200b/200c/429 subfamily as metastasis suppressor microRNAs in human HCC and highlighted the functional discrepancy among miR-200 family members. PMID:25909223

  11. Chemistry 200, 300 Interim Guide.

    ERIC Educational Resources Information Center

    Manitoba Dept. of Education, Winnipeg.

    This guide, developed for the chemistry 200, 300 program in Manitoba, is designed to articulate with previous science courses, provide concepts, processes, and skills which will enable students to continue in chemistry-related areas, and relate chemistry to practical applications in everyday life. It includes a program overview (with program goals…

  12. Physics 200, 300 Interim Guide.

    ERIC Educational Resources Information Center

    Manitoba Dept. of Education, Winnipeg.

    This guide, developed for the physics 200, 300 program in Manitoba, is designed to articulate with previous science courses; provide concepts, processes, and skills which will enable students to continue in physics-related areas; and relate physics to practical applications in everyday life. It includes a program overview (with program goals and…

  13. MicroRNA-200c-141 and ∆Np63 are required for breast epithelial differentiation and branching morphogenesis.

    PubMed

    Hilmarsdóttir, Bylgja; Briem, Eirikur; Sigurdsson, Valgardur; Franzdóttir, Sigrídur Rut; Ringnér, Markus; Arason, Ari Jon; Bergthorsson, Jon Thor; Magnusson, Magnus Karl; Gudjonsson, Thorarinn

    2015-07-15

    The epithelial compartment of the breast contains two lineages, the luminal- and the myoepithelial cells. D492 is a breast epithelial cell line with stem cell properties that forms branching epithelial structures in 3D culture with both luminal- and myoepithelial differentiation. We have recently shown that D492 undergo epithelial to mesenchymal transition (EMT) when co-cultured with endothelial cells. This 3D co-culture model allows critical analysis of breast epithelial lineage development and EMT. In this study, we compared the microRNA (miR) expression profiles for D492 and its mesenchymal-derivative D492M. Suppression of the miR-200 family in D492M was among the most profound changes observed. Exogenous expression of miR-200c-141 in D492M reversed the EMT phenotype resulting in gain of luminal but not myoepithelial differentiation. In contrast, forced expression of ∆Np63 in D492M restored the myoepithelial phenotype only. Co-expression of miR-200c-141 and ∆Np63 in D492M restored the branching morphogenesis in 3D culture underlining the requirement for both luminal and myoepithelial elements for obtaining full branching morphogenesis in breast epithelium. Introduction of a miR-200c-141 construct in both D492 and D492M resulted in resistance to endothelial induced EMT. In conclusion, our data suggests that expression of miR-200c-141 and ∆Np63 in D492M can reverse EMT resulting in luminal- and myoepithelial differentiation, respectively, demonstrating the importance of these molecules in epithelial integrity in the human breast. PMID:25967125

  14. 75 FR 81427 - Airworthiness Directives; The Boeing Company Model 747-200C, -200F, -400, -400D, and -400F Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ..., Amendment 39-14506 (71 FR 12122, March 9, 2006). The existing AD applies to certain Model 747-200C, -200F... (71 FR 12122, March 9, 2006), the Director of the Federal Register approved the incorporation by..., 2010 (75 FR 13046). That NPRM proposed to continue to require repetitive inspections for cracks in...

  15. 75 FR 1533 - Airworthiness Directives; The Boeing Company Model 747-200F, 747-200C, 747-400, 747-400D, and 747...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-12

    ..., 2004, as of April 6, 2006 (71 FR 10605, March 2, 2006). ADDRESSES: For service information identified..., Amendment 39-14499 (71 FR 10605, March 2, 2006). The existing AD applies to all Model 747-200F, 747-200C... July 23, 2009 (74 FR 36417). That NPRM proposed to continue to require repetitive inspections...

  16. Correlation between EGFR Amplification and the Expression of MicroRNA-200c in Primary Glioblastoma Multiforme

    PubMed Central

    Serna, Eva; Lopez-Gines, Concha; Monleon, Daniel; Muñoz-Hidalgo, Lisandra; Callaghan, Robert C.; Gil-Benso, Rosario; Martinetto, Horacio; Gregori-Romero, Aurelia; Gonzalez-Darder, Jose; Cerda-Nicolas, Miguel

    2014-01-01

    Extensive infiltration of the surrounding healthy brain tissue is a critical feature in glioblastoma. Several miRNAs have been related to gliomagenesis, some of them related with the EGFR pathway. We have evaluated whole-genome miRNA expression profiling associated with different EGFR amplification patterns, studied by fluorescence in situ hybridization in tissue microarrays, of 30 cases of primary glioblastoma multiforme, whose clinicopathological and immunohistochemical features have also been analyzed. MicroRNA-200c showed a very significant difference between tumors having or not EGFR amplification. This microRNA plays an important role in epithelial-mesenchymal transition, but its implication in the behavior of glioblastoma is largely unknown. With respect to EGFR status our cases were categorized into three groups: high level EGFR amplification, low level EGFR amplification, and no EGFR amplification. Our results showed that microRNA-200c and E-cadherin expression are down-regulated, while ZEB1 is up-regulated, when tumors showed a high level of EGFR amplification. Conversely, ZEB1 mRNA expression levels were significantly lower in the group of tumors without EGFR amplification. Tumors with a low level of EGFR amplification showed ZEB1 expression levels comparable to those detected in the group with a high level of amplification. In this study we provide what is to our knowledge the first report of association between microRNA-200c and EGFR amplification in glioblastomas. PMID:25058589

  17. Role for DNA methylation in the regulation of miR-200c and miR-141 expression in normal and cancer cells

    SciTech Connect

    Vrba, Lukas; Jensen, Taylor J.; Garbe, James C.; Heimark, Ronald L.; Cress, Anne E.; Dickinson, Sally; Stampfer, Martha R.; Futscher, Bernard W.

    2009-12-23

    BACKGROUND: The microRNA-200 family participates in the maintenance of an epithelial phenotype and loss of its expression can result in epithelial to mesenchymal transition (EMT). Furthermore, the loss of expression of miR-200 family members is linked to an aggressive cancer phenotype. Regulation of the miR-200 family expression in normal and cancer cells is not fully understood. METHODOLOGY/ PRINCIPAL FINDINGS: Epigenetic mechanisms participate in the control of miR-200c and miR-141 expression in both normal and cancer cells. A CpG island near the predicted mir-200c/mir-141 transcription start site shows a striking correlation between miR-200c and miR-141 expression and DNA methylation in both normal and cancer cells, as determined by MassARRAY technology. The CpG island is unmethylated in human miR-200/miR-141 expressing epithelial cells and in miR-200c/miR-141 positive tumor cells. The CpG island is heavily methylated in human miR-200c/miR-141 negative fibroblasts and miR-200c/miR-141 negative tumor cells. Mouse cells show a similar inverse correlation between DNA methylation and miR-200c expression. Enrichment of permissive histone modifications, H3 acetylation and H3K4 trimethylation, is seen in normal miR-200c/miR-141-positive epithelial cells, as determined by chromatin immunoprecipitation coupled to real-time PCR. In contrast, repressive H3K9 dimethylation marks are present in normal miR-200c/miR-141-negative fibroblasts and miR-200c/miR-141 negative cancer cells and the permissive histone modifications are absent. The epigenetic modifier drug, 5-aza-2'-deoxycytidine, reactivates miR-200c/miR-141 expression showing that epigenetic mechanisms play a functional role in their transcriptional control. CONCLUSIONS/ SIGNIFICANCE: We report that DNA methylation plays a role in the normal cell type-specific expression of miR-200c and miR-141 and this role appears evolutionarily conserved, since similar results were obtained in mouse. Aberrant DNA methylation of the

  18. The ZEB1/miR-200c feedback loop regulates invasion via actin interacting proteins MYLK and TKS5

    PubMed Central

    Stemmler, Marc P.; Kleemann, Julia A.; Brabletz, Thomas; Brabletz, Simone

    2015-01-01

    Epithelial to mesenchymal transition (EMT) is a developmental process which is aberrantly activated during cancer invasion and metastasis. Elevated expression of EMT-inducers like ZEB1 enables tumor cells to detach from the primary tumor and invade into the surrounding tissue. The main antagonist of ZEB1 in controlling EMT is the microRNA-200 family that is reciprocally linked to ZEB1 in a double negative feedback loop. Here, we further elucidate how the ZEB1/miR-200 feedback loop controls invasion of tumor cells. The process of EMT is attended by major changes in the actin cytoskeleton. Via in silico screening of genes encoding for actin interacting proteins, we identified two novel targets of miR-200c - TKS5 and MYLK (MLCK). Co-expression of both genes with ZEB1 was observed in several cancer cell lines as well as in breast cancer patients and correlated with low miR-200c levels. Depletion of TKS5 or MYLK in breast cancer cells reduced their invasive potential and their ability to form invadopodia. Whereas TKS5 is known to be a major component, we could identify MYLK as a novel player in invadopodia formation. In summary, TKS5 and MYLK represent two mediators of invasive behavior of cancer cells that are regulated by the ZEB1/miR-200 feedback loop. PMID:26334100

  19. miR-200c Regulation of Metastases in Ovarian Cancer: Potential Role in Epithelial and Mesenchymal Transition

    PubMed Central

    Sulaiman, Siti A.; Ab Mutalib, Nurul-Syakima; Jamal, Rahman

    2016-01-01

    Among the gynecological malignancies, ovarian cancer is the most fatal due to its high mortality rate. Most of the identified cases are epithelial ovarian cancer (EOC) with five distinct subtypes: high-grade serous carcinoma, low-grade serous carcinoma, mucinous carcinoma, endometrioid carcinoma, and clear-cell carcinoma. Lack of an early diagnostic approach, high incidence of tumor relapse and the heterogenous characteristics between each EOC subtypes contribute to the difficulties in developing precise intervention and therapy for the patients. MicroRNAs (miRNAs) are single-stranded RNAs that have been shown to function as tumor suppressors or oncomiRs. The miR-200 family, especially miR-200c, has been shown to be implicated in the metastasis and invasion of ovarian carcinoma due to its functional regulation of epithelial-to-mesenchymal transition (EMT). This mini review is aimed to summarize the recent findings of the miR-200c functional role as well as its validated targets in the metastasis cascade of ovarian cancer, with a focus on EMT regulation. The potential of this miRNA in early diagnosis and its dual expression status are also discussed. PMID:27601996

  20. miR-200c-SUMOylated KLF4 feedback loop acts as a switch in transcriptional programs that control VSMC proliferation.

    PubMed

    Zheng, Bin; Bernier, Michel; Zhang, Xin-hua; Suzuki, Toru; Nie, Chan-quan; Li, Yong Hui; Zhang, Yong; Song, Li-Li; Shi, Hui-jing; Liu, Yan; Zheng, Cui-ying; Wen, Jin-kun

    2015-05-01

    The regulation of vascular smooth muscle cell (VSMC) proliferation is an important issue because it has major implications for the prevention of pathological vascular conditions. Using microRNA array screen, we found the expression levels of 200 unique miRNAs in hyperplasic tissues. Among them, miR-200c expression substantially was down-regulated. The objective of this work was to assess the function of miR-200c and SUMOylated Krϋppel-like transcription factor 4 (KLF4) in the regulation of VSMC proliferation in both cultured cells and animal models of balloon injury. Under basal conditions, we found that miR-200c inhibited the expression of KLF4 and the SUMO-conjugating enzyme Ubc9. Upon PDGF-BB treatment, Ubc9 interacted with and promoted the SUMOylation of KLF4, which allowed the recruitment of transcriptional corepressors (e.g., nuclear receptor corepressor (NCoR) and HDAC2) to the miR-200c promoter. The reduction in miR-200c levels led to increased target gene expression (e.g., Ubc9 and KLF4), which further repressed miR-200c levels and accelerated VSMC proliferation. These results demonstrate that induction of a miR-200c-SUMOylated KLF4 feedback loop is a significant aspect of the PDGF-BB proliferative response in VSMCs and that targeting Ubc9 represents a novel approach for the prevention of restenosis. PMID:25791170

  1. miR200c attenuates P-gp-mediated MDR and metastasis by targeting JNK2/c-Jun signaling pathway in colorectal cancer.

    PubMed

    Sui, Hua; Cai, Guo-Xiang; Pan, Shu-Fang; Deng, Wan-Li; Wang, Yu-Wei; Chen, Zhe-Sheng; Cai, San-Jun; Zhu, Hui-Rong; Li, Qi

    2014-12-01

    MicroRNA-200c (miR200c) recently emerged as an important regulator of tumorigenicity and cancer metastasis; however, its role in regulating multidrug resistance (MDR) remains unknown. In the current study, we found that the expression levels of miR200c in recurrent and metastatic colorectal cancers were significantly lower, whereas the JNK2 expression was higher compared with primary tumors. We showed that in MDR colorectal cancer cells, miR200c targeted the 3' untranslated region of the JNK2 gene. Overexpression of miR200c attenuated the levels of p-JNK, p-c-Jun, P-gp, and MMP-2/-9, the downstream factors of the JNK signaling pathway, resulting in increased sensitivity to chemotherapeutic drugs, which was accompanied by heightened apoptosis and decreased cell invasion and migration. Moreover, in an orthotopic MDR colorectal cancer mouse model, we demonstrated that overexpression of miR200c effectively inhibited the tumor growth and metastasis. At last, in the tumor samples from patients with locally advanced colorectal cancer with routine postsurgical chemotherapy, we observed an inverse correlation between the levels of mRNA expression of miR200c and JNK2, ABCB1, and MMP-9, thus predicting patient therapeutic outcomes. In summary, we found that miR200c negatively regulated the expression of JNK2 gene and increased the sensitivity of MDR colorectal cancer cells to chemotherapeutic drugs, via inhibiting the JNK2/p-JNK/p-c-Jun/ABCB1 signaling. Restoration of miR200c expression in MDR colorectal cancer may serve as a promising therapeutic approach in MDR-induced metastasis. PMID:25205654

  2. Electroneutral composite polymersomes self-assembled by amphiphilic polyphosphazenes for effective miR-200c in vivo delivery to inhibit drug resistant lung cancer.

    PubMed

    Peng, Yan; Zhu, Xiumei; Qiu, Liyan

    2016-11-01

    MiR-200c has been confirmed to display remarkable effects on proliferation inhibition and apoptosis induction of certain cancer cells, but the main challenge for its successful translation into the clinic remains its effective delivery to the action site in vivo. In this study, a novel composite polyphosphazene vesicle system composed of amphiphilic [NP(PEG)0.3(EAB)1.7]n (PEEP) and weakly cationic [NP(PEG)0.5(DPA)1.5]n (PEDP) was prepared via a very simple dialysis method. The loading of miR-200c was accomplished with high efficiency by taking advantage of the combination effect of physical encapsulation and electrostatic interaction between vectors and miR-200c. The resultant miR-200c-loaded PEEP-PEDP polymersome (Nano-ED-200c) displayed suitable particle size, electric neutrality, excellent Ribonuclease stability and hemocompatibility. We also evaluated its subsequent miR-200c function in paclitaxel resistance human lung cancer (A549/T) cells in culture and tumor xenografts in nude mice. The results showed that Nano-ED-200c could achieve a higher miR-200c level and the enhanced antitumor efficacy with 68% tumor inhibition ratio at a very low dose of 1.0 mg/kg than PEEP nanoparticle, PEDP nanoparticle, even than Lipo2000. All these evidences indicated that this miR-200c delivery via polyphosphazene vesicles could act as a potential new therapeutic option for paclitaxel resistant human lung cancer. PMID:27541441

  3. The electrolyte challenge for a direct methanol-air polymer electrolyte fuel cell operating at temperatures up to 200 C

    NASA Technical Reports Server (NTRS)

    Savinell, Robert; Yeager, Ernest; Tryk, Donald; Landau, Uziel; Wainright, Jesse; Gervasio, Dominic; Cahan, Boris; Litt, Morton; Rogers, Charles; Scherson, Daniel

    1993-01-01

    Novel polymer electrolytes are being evaluated for use in a direct methanol-air fuel cell operating at temperatures in excess of 100 C. The evaluation includes tests of thermal stability, ionic conductivity, and vapor transport characteristics. The preliminary results obtained to date indicate that a high temperature polymer electrolyte fuel cell is feasible. For example, Nafion 117 when equilibrated with phosphoric acid has a conductivity of at least 0.4 Omega(exp -1)cm(exp -1) at temperatures up to 200 C in the presence of 400 torr of water vapor and methanol vapor cross over equivalent to 1 mA/cm(exp 2) under a one atmosphere methanol pressure differential at 135 C. Novel polymers are also showing similar encouraging results. The flexibility to modify and optimize the properties by custom synthesis of these novel polymers presents an exciting opportunity to develop an efficient and compact methanol fuel cell.

  4. miR-200c/Bmi1 axis and epithelial–mesenchymal transition contribute to acquired resistance to BRAF inhibitor treatment

    PubMed Central

    Liu, Shujing; Tetzlaff, Michael T.; Wang, Tao; Yang, Ruifeng; Xie, Lin; Zhang, Gao; Krepler, Clemens; Xiao, Min; Beqiri, Marilda; Xu, Wei; Karakousis, Giorgos; Schuchter, Lynn; Amaravadi, Ravi K.; Xu, Weiting; Wei, Zhi; Herlyn, Meenhard; Yao, Yuan; Zhang, Litao; Wang, Yingjie; Zhang, Lin; Xu, Xiaowei

    2015-01-01

    Summary Resistance to BRAF inhibitors (BRAFi) is one of the major challenges for targeted therapies for BRAF-mutant melanomas. However, little is known about the role of microRNAs in conferring BRAFi resistance. Herein, we demonstrate that miR-200c expression is significantly reduced whereas miR-200c target genes including Bmi1, Zeb2, Tubb3, ABCG5, and MDR1 are significantly increased in melanomas that acquired BRAFi resistance compared to pretreatment tumor biopsies. Similar changes were observed in BRAFi-resistant melanoma cell lines. Overexpression of miR-200c or knock-down of Bmi1 in resistant melanoma cells restores their sensitivities to BRAFi, leading to deactivation of the PI3K/AKT and MAPK signaling cascades, and acquisition of epithelial– mesenchymal transition-like phenotypes, including upregulation of E-cadherin, downregulation of N-cadherin, and ABCG5 and MDR1 expression. Conversely, knock-down of miR-200c or overexpression of Bmi1 in BRAFi-sensitive melanoma cells activates the PI3K/AKT and MAPK pathways, upregulates N-cadherin, ABCG5, and MDR1 expression, and downregulates E-cadherin expression, leading to BRAFi resistance. Together, our data identify miR-200c as a critical signaling node in BRAFi-resistant melanomas impacting the MAPK and PI3K/AKT pathways, suggesting miR-200c as a potential therapeutic target for overcoming acquired BRAFi resistance. PMID:25903073

  5. MiR-200c overexpression is associated with better efficacy of EGFR-TKIs in non-small cell lung cancer patients with EGFR wild-type

    PubMed Central

    Li, Jiayu; Li, Xuefei; Ren, Shengxiang; Chen, Xiaoxia; Zhang, Yishi; Zhou, Fei; Zhao, Mingchuan; Zhao, Chao; Chen, Xiu; Cheng, Ningning; Zhao, Yinmin; Zhou, Caicun; Hirsch, Fred R.

    2014-01-01

    Several randomized trials have demonstrated non-small cell lung cancer (NSCLC) patients with activating epidermal growth factor receptor (EGFR) mutations can achieve favorable clinical outcomes on treatment with EGFR tyrosine kinase inhibitors (TKIs). EGFR mutation is considered as a predictive marker for efficacy of EGFR-TKIs in NSCLC. Here we show miR-200c overexpression was correlated with the epithelial phenotype and sensitivity to gefitinib in EGFR wild-type NSCLC cell lines. Up-regulated miR-200c could regain the sensitivity to gefitinib in the EGFR wild-type cell lines and miR-200c could regulate epithelial to mesenchymal transition through PI3K/AKT and MEK/ERK pathways. NSCLC patients at advanced stage (N=150) who received EGFR-TKIs (gefitinib or erlotinib) as second- or third-line therapy from September 2008 to December 2012 were included in the study. In 66 NSCLC patients with wild-type EGFR, high levels of miR-200c expression was associated with higher disease control rate (DCR), longer progression-free survival (PFS) and longer overall survival (OS) compared with low miR-200c expression subgroup. In the subgroup with EGFR mutation, the trend remained the same but not statistically significant. Overall, these findings indicated that miR-200c might be a predictive biomarker for sensitivity to EGFR-TKIs in advanced NSCLC patients with wild-type EGFR. PMID:25277203

  6. Diagnostic and prognostic relevance of circulating exosomal miR-373, miR-200a, miR-200b and miR-200c in patients with epithelial ovarian cancer

    PubMed Central

    Meng, Xiaodan; Müller, Volkmar; Milde-Langosch, Karin; Trillsch, Fabian; Pantel, Klaus; Schwarzenbach, Heidi

    2016-01-01

    Exosomes are membrane vesicles that mediate intercellular communication by transporting their molecular cargo from cell to cell. We investigated whether serum levels of exosomal miR-373, miR-200a, miR-200b and miR-200c and circulating exosomes have diagnostic and prognostic relevance in a cohort of 163 epithelial ovarian cancer (EOC) patients using TaqMan MicroRNA assays and ELISA. The serum concentrations of exosomal miR-373 (p = 0.0001), miR-200a (p = 0.0001), miR-200b (p = 0.0001) and miR-200c (p = 0.028) were significantly higher in EOC patients than healthy women. The levels of miR-200a (p = 0.0001), miR-200b (p = 0.0001) and miR-200c (p = 0.019) could distinguish between malignant and benign ovarian tumors. While the levels of miR-373 and miR-200a were increased in all FIGO/lymph node stages (p = 0.0001), the levels of miR-200b and miR-200c were higher in patients with FIGO stage III–IV (p = 0.0001, p = 0.008, respectively) including lymph node metastasis (p = 0.0001, p = 0.004, respectively) than FIGO stages I–II. The increased levels of miR-200b and miR-200c were also associated with CA125 values (p = 0.0001, p = 0.0001, respectively) and a shorter overall survival (p = 0.007, p = 0.017, respectively). The levels of exosomes were excessively elevated in EOC patients (p = 0.0001). In all three cohorts, they were positively associated with the serum levels of exosomal miR-373 (p = 0.004), miR-200a (p = 0.0001), miR-200b (p = 0.0001) and miR-200c (p = 0.008). In conclusion, the increased levels of exosomal miR-200b and miR-200c mainly observed in advanced EOC suggest that these microRNAs may be involved in tumor progression. The high concentrations of exosomes in EOC patients imply an excessive, active exosomal secretion in EOC. PMID:26943577

  7. Enhancement of radiotherapy efficacy by miR-200c-loaded gelatinase-stimuli PEG-Pep-PCL nanoparticles in gastric cancer cells.

    PubMed

    Cui, Fang-bo; Liu, Qin; Li, Ru-Tian; Shen, Jie; Wu, Pu-yuan; Yu, Li-Xia; Hu, Wen-jing; Wu, Feng-lei; Jiang, Chun-Ping; Yue, Guo-feng; Qian, Xiao-Ping; Jiang, Xi-Qun; Liu, Bao-Rui

    2014-01-01

    Radiotherapy is the main locoregional control modality for many types of unresectable tumors, including gastric cancer. However, many patients fail radiotherapy due to intrinsic radioresistance of cancer cells, which has been found to be strongly associated with cancer stem cell (CSC)-like properties. In this study, we developed a nanoparticle formulation to deliver miR-200c, which is reported to inhibit CSC-like properties, and then evaluated its potential activity as a radiosensitizer. miR-200c nanoparticles significantly augmented radiosensitivity in three gastric cancer cell lines (sensitization enhancement ratio 1.13-1.25), but only slightly in GES-1 cells (1.06). In addition to radioenhancement, miR-200c nanoparticles reduced the expression of CD44, a putative CSC marker, and the percentage of CD44(+) BGC823 cells. Meanwhile, other CSC-like properties, including invasiveness and resistance to apoptosis, could be suppressed by miR-200c nanoparticles. CSC-associated radioresistance mechanisms, involving reactive oxygen species levels and DNA repair capacity, were also attenuated. We have demonstrated that miR-200c nanoparticles are an effective radiosensitizer in gastric cancer cells and induce little radiosensitization in normal cells, which suggests that they are as a promising candidate for further preclinical and clinical evaluation. PMID:24872697

  8. Knockdown of lncRNA-ATB suppresses autocrine secretion of TGF-β2 by targeting ZNF217 via miR-200c in keloid fibroblasts

    PubMed Central

    Zhu, Hua-Yu; Bai, Wen-Dong; Li, Chao; Zheng, Zhao; Guan, Hao; Liu, Jia-Qi; Yang, Xue-Kang; Han, Shi-Chao; Gao, Jian-Xin; Wang, Hong-Tao; Hu, Da-Hai

    2016-01-01

    Abnormally high activation of transforming growth factor-β (TGF-β) signaling has been demonstrated to be involved in the initiation and progression of keloids. However, the functional role of long non-coding RNA (lncRNA)-activated by TGF-β (lncRNA-ATB) in keloids has not been documented. Here we investigated the role of lncRNA-ATB in the autocrine secretion of TGF-β in keloid fibroblasts (KFs) and explored the underlying molecular mechanism. Using immunohistochemistry and quantitative RT-PCR analysis, we showed that lncRNA-ATB and ZNF217, a transcriptional activator of TGF-β, were overexpressed and miR-200c, which targets ZNF217, was under-expressed in keloid tissue and keloid fibroblasts. Through gain- and loss-of-function studies, we demonstrated that knockdown of lncRNA-ATB decreased autocrine secretion of TGF-β2 and ZNF217 expression but upregulated expression of miR-200c in KFs. Stable downregulation of ZNF217 expression decreased the autocrine secretion of TGF-β2. miR-200c was endogenously associated with lncRNA-ATB, and inhibition of miR-200c overcame the decrease in ZNF217 expression in KFs. Taken together, these findings indicate that lncRNA-ATB governs the autocrine secretion of TGF-β2 in KFs, at least in part, by downregulating the expression level of ZNF217 via miR-200c, suggesting a signaling axis consisting of lncRNA-ATB/miR-200c/ZNF217/TGF-β2. These findings may provide potential biomarkers and targets for novel diagnostic and therapeutic approaches for keloids. PMID:27090737

  9. Knockdown of lncRNA-ATB suppresses autocrine secretion of TGF-β2 by targeting ZNF217 via miR-200c in keloid fibroblasts.

    PubMed

    Zhu, Hua-Yu; Bai, Wen-Dong; Li, Chao; Zheng, Zhao; Guan, Hao; Liu, Jia-Qi; Yang, Xue-Kang; Han, Shi-Chao; Gao, Jian-Xin; Wang, Hong-Tao; Hu, Da-Hai

    2016-01-01

    Abnormally high activation of transforming growth factor-β (TGF-β) signaling has been demonstrated to be involved in the initiation and progression of keloids. However, the functional role of long non-coding RNA (lncRNA)-activated by TGF-β (lncRNA-ATB) in keloids has not been documented. Here we investigated the role of lncRNA-ATB in the autocrine secretion of TGF-β in keloid fibroblasts (KFs) and explored the underlying molecular mechanism. Using immunohistochemistry and quantitative RT-PCR analysis, we showed that lncRNA-ATB and ZNF217, a transcriptional activator of TGF-β, were overexpressed and miR-200c, which targets ZNF217, was under-expressed in keloid tissue and keloid fibroblasts. Through gain- and loss-of-function studies, we demonstrated that knockdown of lncRNA-ATB decreased autocrine secretion of TGF-β2 and ZNF217 expression but upregulated expression of miR-200c in KFs. Stable downregulation of ZNF217 expression decreased the autocrine secretion of TGF-β2. miR-200c was endogenously associated with lncRNA-ATB, and inhibition of miR-200c overcame the decrease in ZNF217 expression in KFs. Taken together, these findings indicate that lncRNA-ATB governs the autocrine secretion of TGF-β2 in KFs, at least in part, by downregulating the expression level of ZNF217 via miR-200c, suggesting a signaling axis consisting of lncRNA-ATB/miR-200c/ZNF217/TGF-β2. These findings may provide potential biomarkers and targets for novel diagnostic and therapeutic approaches for keloids. PMID:27090737

  10. 50 CFR 300.200 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Definition of Illegal, Unreported, or Unregulated Fishing § 300.200 Purpose. The purpose of this subpart is... Reauthorization Act of 2006 (“Act”) to publish a definition of the term “Illegal, unreported, or...

  11. 20 CFR 664.205 - How is the “deficient in basic literacy skills” criterion in § 664.200(c)(1) defined and documented?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 4 2013-04-01 2013-04-01 false How is the âdeficient in basic literacy... WORKFORCE INVESTMENT ACT Eligibility for Youth Services § 664.205 How is the “deficient in basic literacy... documentation requirements regarding the “deficient in basic literacy skills” criterion in § 664.200(c)(1)...

  12. 20 CFR 664.205 - How is the “deficient in basic literacy skills” criterion in § 664.200(c)(1) defined and documented?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 4 2012-04-01 2012-04-01 false How is the âdeficient in basic literacy... WORKFORCE INVESTMENT ACT Eligibility for Youth Services § 664.205 How is the “deficient in basic literacy... documentation requirements regarding the “deficient in basic literacy skills” criterion in § 664.200(c)(1)...

  13. 20 CFR 664.205 - How is the “deficient in basic literacy skills” criterion in § 664.200(c)(1) defined and documented?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false How is the âdeficient in basic literacy... INVESTMENT ACT Eligibility for Youth Services § 664.205 How is the “deficient in basic literacy skills... requirements regarding the “deficient in basic literacy skills” criterion in § 664.200(c)(1) may be...

  14. Transcriptomic Profiling Reveals Hepatic Stem-like Gene Signatures and Interplay of miR-200c and EMT in Intrahepatic Cholangiocarcinoma

    PubMed Central

    Oishi, Naoki; Kumar, Mia R.; Roessler, Stephanie; Ji, Junfang; Forgues, Marshonna; Budhu, Anuradha; Zhao, Xuelian; Andersen, Jesper B.; Ye, Qing-Hai; Jia, Hu-Liang; Qin, Lun-Xiu; Yamashita, Taro; Woo, Hyun Goo; Kim, Yoon Jun; Kaneko, Shuichi; Tang, Zhao-You; Thorgeirsson, Snorri S.; Wang, Xin Wei

    2012-01-01

    Intrahepatic cholangiocellular carcinoma (ICC) is the second most common type of primary liver cancer. However, its tumor heterogeneity and molecular characteristics are largely unknown. In this study, we conducted transcriptomic profiling of 23 ICC and combined hepatocellular cholangiocarcinoma tumor specimens from Asian patients using Affymetrix mRNA and Nanostring microRNA microarrays to search for unique gene signatures linked to tumor subtypes and patient prognosis. We validated the signatures in additional 68 ICC cases derived from Caucasian patients. We found that both mRNA and microRNA expression profiles could independently classify Asian ICC cases into two main subgroups, one of which shared gene expression signatures with previously identified hepatocellular carcinoma (HCC) with stem cell gene expression traits. ICC-specific gene signatures could predict survival in Asian HCC cases and independently in Caucasian ICC cases. Integrative analyses of the ICC-specific mRNA and microRNA expression profiles revealed that a common signaling pathway linking miR-200c signaling to epithelial-mesenchymal transition (EMT) was preferentially activated in ICC with stem cell gene expression traits. Inactivation of miR-200c resulted in an induction of EMT while activation of miR-200c led to a reduction of EMT including a reduced cell migration and invasion in ICC cells. We also found that miR-200c and NCAM1 expression were negatively correlated and their expression levels were predictive of survival in ICC samples. NCAM1, a known hepatic stem/progenitor cell marker, was experimentally demonstrated to be a direct target of miR-200c. Conclusion: Our results indicate that ICC and HCC share common stem-like molecular characteristics and poor prognosis. We suggest that the specific components of EMT may be exploited as critical biomarkers and clinically relevant therapeutic targets for an aggressive form of stem cell-like ICC. PMID:22707408

  15. Enzyme Substrate Complex of the H200C Variant of Homoprotocatechuate 2,3-Dioxygenase: Mössbauer and Computational Studies.

    PubMed

    Meier, Katlyn K; Rogers, Melanie S; Kovaleva, Elena G; Lipscomb, John D; Bominaar, Emile L; Münck, Eckard

    2016-06-20

    The extradiol, aromatic ring-cleaving enzyme homoprotocatechuate 2,3-dioxygenase (HPCD) catalyzes a complex chain of reactions that involve second sphere residues of the active site. The importance of the second-sphere residue His200 was demonstrated in studies of HPCD variants, such as His200Cys (H200C), which revealed significant retardations of certain steps in the catalytic process as a result of the substitution, allowing novel reaction cycle intermediates to be trapped for spectroscopic characterization. As the H200C variant largely retains the wild-type active site structure and produces the correct ring-cleaved product, this variant presents a valuable target for mechanistic HPCD studies. Here, the high-spin Fe(II) states of resting H200C and the H200C-homoprotocatechuate enzyme-substrate (ES) complex have been characterized with Mössbauer spectroscopy to assess the electronic structures of the active site in these states. The analysis reveals a high-spin Fe(II) center in a low symmetry environment that is reflected in the values of the zero-field splitting (ZFS) (D ≈ - 8 cm(-1), E/D ≈ 1/3 in ES), as well as the relative orientations of the principal axes of the (57)Fe magnetic hyperfine (A) and electric field gradient (EFG) tensors relative to the ZFS tensor axes. A spin Hamiltonian analysis of the spectra for the ES complex indicates that the magnetization axis of the integer-spin S = 2 Fe(II) system is nearly parallel to the symmetry axis, z, of the doubly occupied dxy ground orbital deduced from the EFG and A-values, an observation, which cannot be rationalized by DFT assisted crystal-field theory. In contrast, ORCA/CASSCF calculations for the ZFS tensor in combination with DFT calculations for the EFG- and A-tensors describe the experimental data remarkably well. PMID:27275865

  16. miR-141 and miR-200c as Markers of Overall Survival in Early Stage Non-Small Cell Lung Cancer Adenocarcinoma

    PubMed Central

    Campayo, Marc; Viñolas, Nuria; Marrades, Ramon M.; Cordeiro, Anna; Ruíz-Martínez, Marc; Santasusagna, Sandra; Molins, Laureano; Ramirez, Josep; Monzó, Mariano

    2014-01-01

    Background Several treatments in non-small cell lung cancer (NSCLC) are histology-dependent, and the need for histology-related markers is increasing. MicroRNAs (miRNAs) are promising molecular markers in multiple cancers and show differences in expression depending on histological subtype. The miRNA family miR-200 has been associated with the regulation of epithelial-mesenchymal (EMT)/mesenchymal-epithelial transition (MET). EMT involves profound phenotypic changes that include the loss of cell-cell adhesion, the loss of cell polarity, and the acquisition of migratory and invasive properties that facilitates metastasis. A dual role for the miR-200 family in the prognosis of several tumors has been related to tumor cell origin. However, the prognostic role and function of miR-200 family in early-stage NSCLC adenocarcinoma and squamous cell carcinoma (SCC) have not been well established. Methods miRNA expression was determined using TaqMan assays in 155 tumors from resected NSCLC patients. Functional studies were conducted in three NSCLC cell lines: H23, A-549 and HCC-44. Results High miR-200c expression was associated with shorter overall survival (OS) in the entire cohort (p = 0.024). High miR-200c (p = 0.0004) and miR-141 (p = 0.009) expression correlated with shorter OS in adenocarcinoma – but not in SCC. In the multivariate analysis, a risk score based on miR-141 and miR-200c expression emerged as an independent prognostic factor for OS in the entire cohort (OR, 2.787; p = 0.033) and in adenocarcinoma patients (OR, 10.649; p = 0.002). Functional analyses showed that miR-200c, was related to mesenchymal-epithelial transition (MET) and affected cell migration and E-cadherin levels, while overexpression of miR-141 reduced KLF6 protein levels and produced an increase of secretion of VEGFA in vitro (H23, p = 0.04; A-549, p = 0.03; HCC-44, p = 0.02) and was associated with higher blood microvessel density in patient tumor samples (p

  17. Detection of Drug-Induced Acute Kidney Injury in Humans Using Urinary KIM-1, miR-21, -200c, and -423.

    PubMed

    Pavkovic, Mira; Robinson-Cohen, Cassianne; Chua, Alicia S; Nicoara, Oana; Cárdenas-González, Mariana; Bijol, Vanesa; Ramachandran, Krithika; Hampson, Lucy; Pirmohamed, Munir; Antoine, Daniel J; Frendl, Gyorgy; Himmelfarb, Jonathan; Waikar, Sushrut S; Vaidya, Vishal S

    2016-07-01

    Drug-induced acute kidney injury (AKI) is often encountered in hospitalized patients. Although serum creatinine (SCr) is still routinely used for assessing AKI, it is known to be insensitive and nonspecific. Therefore, our objective was to evaluate kidney injury molecule 1 (KIM-1) in conjunction with microRNA (miR)-21, -200c, and -423 as urinary biomarkers for drug-induced AKI in humans. In a cross-sectional cohort of patients (n = 135) with acetaminophen (APAP) overdose, all 4 biomarkers were significantly (P < .004) higher not only in APAP-overdosed (OD) patients with AKI (based on SCr increase) but also in APAP-OD patients without clinical diagnosis of AKI compared with healthy volunteers. In a longitudinal cohort of patients with malignant mesothelioma receiving intraoperative cisplatin (Cp) therapy (n = 108) the 4 biomarkers increased significantly (P < .0014) over time after Cp administration, but could not be used to distinguish patients with or without AKI. Evidence for human proximal tubular epithelial cells (HPTECs) being the source of miRNAs in urine was obtained first, by in situ hybridization based confirmation of increase in miR-21 expression in the kidney sections of AKI patients and second, by increased levels of miR-21, -200c, and -423 in the medium of cultured HPTECs treated with Cp and 4-aminophenol (APAP degradation product). Target prediction analysis revealed 1102 mRNA targets of miR-21, -200c, and -423 that are associated with pathways perturbed in diverse pathological kidney conditions. In summary, we report noninvasive detection of AKI in humans by combining the sensitivity of KIM-1 along with mechanistic potentials of miR-21, -200c, and -423. PMID:27122240

  18. 76 FR 6529 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-07

    ...We are adopting a new airworthiness directive (AD) for the products listed above. This AD requires installing two warning level indicator lights on the P2-2 center instrument panel in the flight compartment for certain airplanes. For a certain other airplane, this AD requires activating the cabin altitude warning and takeoff configuration warning lights. For all airplanes, this AD also......

  19. 75 FR 48620 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-11

    ... flightcrew due to hypoxia (lack of oxygen in body), and consequent loss of control of the airplane. DATES: We... warning horn could result in incapacitation of the flightcrew due to hypoxia (lack of oxygen in body), and... November 7, 2003, we issued related AD 2003-03-15 R1, Amendment 39-13366 (68 FR 64802, November 17,...

  20. 75 FR 9137 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-01

    ... energy aspects of this proposed AD. We will consider all comments received by the closing date and may... November 24, 2003, we issued AD 2003-24-08, Amendment 39-13377 (68 FR 67027, December 1, 2003), for all... Average U.S.- Action Work hours labor rate Parts Cost per airplane registered Fleet cost per...

  1. 75 FR 43803 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-27

    ..., 2010. On December 4, 2003 (68 FR 67027, December 1, 2003), the Director of the Federal Register... include an AD that supersedes AD 2003-24-08, Amendment 39-13377 (68 FR 67027, December 1, 2003). The... published in the Federal Register on March 1, 2010 (75 FR 9137). That NPRM proposed to continue to...

  2. 76 FR 74667 - Airworthiness Directives; The Boeing Company Model 737-200, -200C, -300, -400, and -500 Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-01

    ... publication listed in this AD as of February 16, 2010 (75 FR 1527, January 12, 2010). ADDRESSES: For service... (NPRM) to amend 14 CFR part 39 to supersede AD 2010-01-09, Amendment 39-16167 (75 FR 1527, January 12... September 1, 2011 (76 FR 54399). That NPRM proposed to continue to require repetitive external...

  3. 76 FR 3856 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-21

    ..., replacing the fuel pump power control relays for the main, center and auxiliary tanks, as applicable, with...'' (66 FR 23086, May 7, 2001). In addition to new airworthiness standards for transport airplanes and new... fuel boost pumps, the center tank fuel boost pumps, and for certain airplanes, the auxiliary tank...

  4. 76 FR 33988 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-10

    ... changes, replacing the fuel pump power control relays for the main, center, and auxiliary tanks, as... (76 FR 3856). That NPRM proposed to require, depending on airplane configuration, doing certain wiring changes, replacing the fuel pump power control relays for the main, center, and auxiliary tanks,...

  5. 76 FR 63229 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-12

    ... System Design Review, Flammability Reduction and Maintenance and Inspection Requirements'' (66 FR 23086.... Other Relevant Rulemaking On April 18, 2001, we issued AD 2001-08-24, Amendment 39-12201 (66 FR 20733... 16, 2009, we issued AD 2008-10-09 R1, Amendment 39- 16148 (74 FR 69264, December 31, 2009), for...

  6. 75 FR 19564 - Airworthiness Directives; The Boeing Company Model 737-200, -200C, -300, -400, and- 500 Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-15

    ..., are necessary to comply with AD 90-06-02, Amendment 39-6489 (55 FR 8372, March 7, 1990). FAA's... Order 12866, 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR... surrounding structure under the number 3 very high frequency (VHF) antenna, and corrective actions...

  7. 75 FR 10658 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-09

    ... 2006-07-12, Amendment 39-14539 (71 FR 16211, March 31, 2006). The existing AD applies to all Model 737... Register on May 20, 2009 (74 FR 23664). That NPRM proposed to expand the area to be inspected and, for..., Revision 2, dated October 25, 2006, mandated by AD 2003-14-06, Amendment 39-13225 (68 FR 42956, July...

  8. 76 FR 47522 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-05

    .... Discussion On June 5, 1995, we issued AD 95-12-17, amendment 39-9268 (60 FR 36981, July 19, 1995), for... produced with outboard chords at BS 727 made of 7075-T6 aluminum; subsequent airplanes were produced with outboard chords made of 7075-T73 aluminum. In addition, we have determined that the inspections of...

  9. 75 FR 17889 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-08

    ... rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979), and 3. Will... Model 777-200, - 200LR, -300, and -300ER Series Airplanes AGENCY: Federal Aviation Administration (FAA... directive (AD) for certain Model 777-200, -200LR, -300, and -300ER series airplanes. This proposed AD...

  10. NF-κB-mediated inflammation leading to EMT via miR-200c is involved in cell transformation induced by cigarette smoke extract.

    PubMed

    Zhao, Yue; Xu, Yuan; Li, Yuan; Xu, Wenchao; Luo, Fei; Wang, Bairu; Pang, Ying; Xiang, Quanyong; Zhou, Jianwei; Wang, Xinru; Liu, Qizhan

    2013-10-01

    Cigarette smoking constitutes a major human health hazard because it is the most important risk factor for lung cancer. Although evidence for smoking-induced lung cancer in humans is strong, the molecular mechanisms by which smoking causes cancer remain to be established. In this investigation, we evaluated the roles of inflammation and the epithelial-mesenchymal transition (EMT) in cigarette smoke extract (CSE)-induced transformation of human bronchial epithelial (HBE) cells. The results showed that chronic exposure to CSE induced EMT and transformation of these cells. Activation of nuclear factor-κB (NF-κB) by CSE increased levels of the proinflammatory interleukin-6 (IL-6), and acute and chronic exposures to CSE caused decreases in miR-200c levels. By blocking NF-κB with Bay11-7082 and IL-6 with anti-IL-6 antibody and enhancement of IL-6 with human recombinant IL-6, we found that the NF-κB signal pathway was involved in CSE-induced increases of IL-6, which suppressed miR-200c expression and promoted EMT. Moreover, IL-6 was necessary for maintenance of CSE-induced transformation and for malignant progression of HBE cells. Finally, blocking of NF-κB with Bay11-7082 prevented CSE-induced EMT and malignant transformation due to decreases of E-cadherin and miR-200c and elevations of IL-6, N-cadherin, and vimentin. Thus, we have defined a link between inflammation and EMT, processes involved in the malignant transformation of cells caused by CSE. This link, mediated through miRNAs, establishes a mechanism for CSE-induced lung carcinogenesis. PMID:23824089

  11. Combining TGF-β1 knockdown and miR200c administration to optimize antitumor efficacy of B16F10/GPI-IL-21 vaccine

    PubMed Central

    Wang, Jing; Zhang, Ying; Zhang, Hongyi; Ni, Yaoyao; Sun, Jianan; Wang, Xiaobing; Dou, Jun

    2015-01-01

    TGF-β1 secreted abundantly by tumors cells as well as present in the local microenvironment promotes neoplasm invasion and metastasis by triggering the epithelial to mesenchymal transition (EMT). MiR200c has been shown to suppress EMT and to regulate the cellular epithelial and interstitial state conversion, whereas the tumor vaccines are intended to specifically initiate or amplify a host response against evolving tumor cells. Our study aimed at optimizing the antitumor effects of the B16F10/glycosylphosphatidylinositol-interleukin 21 (B16F10/GPI-IL-21) tumor vaccine on melanoma bearing mice by combining the TGF-β1 knockdown and the administration of miR200c agomir. The mice were subcutaneously vaccinated with inactivated B16F10/GPI-IL-21 vaccine and challenged by B16F10 cells transfected with shTGF-β1 (B16F10/shTGF-β1 cells) or B16F10/shTGF-β1 cells with the administration of miR200c agomir. The later combination showed that, when compared with the mice in the control group that received no vaccination, vaccinated mice significantly increased NK and CTL activities, enhanced levels of IFN-γ, and reduced expression of TGF-β1, N-cadherin, Vimentin, Gli1/2, P-Smad2/3 and others involved in promoting expression of EMT-related molecules in tumor areas, and inhibited the melanoma metastasis in lungs and lymph nodes. Altogether, our findings demonstrate that this synergistic anti-cancer regimen effectively induces strong immune response and diminishes the melanoma progression. PMID:25895132

  12. Demethylation drug 5-Aza-2′-deoxycytidine-induced upregulation of miR-200c inhibits the migration, invasion and epithelial-mesenchymal transition of clear cell renal cell carcinoma in vitro

    PubMed Central

    JIANG, JUAN; YI, BO; DING, SIQING; SUN, JIAN; CAO, WEI; LIU, MENGZI

    2016-01-01

    The microRNA (miR)-200 family has been found to be involved in the process of mesenchymal-epithelial transition during renal development. Deregulation of miR-200c has been suggested to be involved in clear cell renal cell carcinoma (ccRCC). However, the precise role of miR-200c in the regulation of ccRCC metastasis has not been previously reported. In the present study, it was observed that miR-200c was frequently downregulated in ccRCC tissue compared with matched adjacent normal tissue. The expression of miR-200c was additionally reduced in ccRCC cell lines when compared with levels in normal renal cells. The DNA demethylation drug 5-Aza-2′-deoxycytidine (Aza) was used to treat several ccRCC cell lines, and it was observed that the expression of miR-200c was significantly increased following Aza treatment. Furthermore, treatment with Aza markedly inhibited ccRCC cell invasion and migration, while treatment with miR-200c inhibitor significantly enhanced invasion and migration of ccRCC cells. In addition, Aza treatment significantly promoted expression of E-cadherin and inhibited the expression of N-cadherin, while the inhibition of miR-200c downregulated E-cadherin and upregulated the expression of N-cadherin, suggesting that miR-200c has a suppressive role in epithelial-mesenchymal transition (EMT) of ccRCC cells. In conclusion, it was suggested that demethylation drug Aza-induced upregulation of miR-200c may inhibit migration, invasion and EMT in ccRCC cells. PMID:27123083

  13. Integrated Analysis Reveals together miR-182, miR-200c and miR-221 Can Help in the Diagnosis of Prostate Cancer

    PubMed Central

    Qin, Xia; Chen, Panyu; Zou, Yi ming; Hu, Yanling

    2015-01-01

    Research has shown that microRNAs are promising biomarkers that can be used to promote a more accurate diagnosis of cancer. In this study, we developed an integrated multi-step selection process to analyze available high-throughput datasets to obtain information on microRNAs as cancer biomarkers. Applying this approach to the microRNA expression profiles of prostate cancer and the datasets in The Cancer Genome Atlas Data Portal, we identified miRNA-182, miRNA-200c and miRNA-221 as possible biomarkers for prostate cancer. The associations between the expressions of these three microRNAs with clinical parameters as well as their diagnostic capability were studied. Several online databases were used to predict the target genes of these three microRNAs, and the results were confirmed by significant statistical correlations. Comparing with the other 18 types of cancers listed in The Cancer Genome Atlas Data Portal, we found that the combination of both miRNA-182 and miRNA-200c being up-regulated and miRNA-221 being down-regulated only happens in prostate cancer. This provides a unique biological characteristic for prostate cancer that can potentially be used for diagnosis based on tissue testing. In addition, our study also revealed that these three microRNAs are associated with the pathological status of prostate cancer. PMID:26484677

  14. 24 CFR 200.300 - Nondiscrimination and fair housing policy.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Nondiscrimination and fair housing policy. 200.300 Section 200.300 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN...

  15. 24 CFR 200.300 - Nondiscrimination and fair housing policy.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Nondiscrimination and fair housing policy. 200.300 Section 200.300 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN...

  16. Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir.

    PubMed

    Wang, Xiaoying; Zhao, Fengshu; Shi, Fangfang; He, Xiangfeng; Pan, Meng; Wu, Di; Li, Miao; Zhang, Yunxia; Dou, Jun

    2016-05-01

    In this study, we hypothesized that the inhibition of epithelial to mesenchymal transition (EMT) program by knockdown of Zinc-finger E-box binding homeobox 1 (ZEB1) or administration of miR200c agomir would strengthen the B16F10 cells transfected with GPI-anchored IL-21 (B16F10/GPI-IL-21) vaccine efficacy in inhibiting the melanoma metastasis. Our findings from the current study indicated that, when compared with the mice immunized with the B16F10/GPI-IL-21 vaccine alone, the mice immunized with B16F10/GPI-IL-21 vaccine combined with injection of shZEB1 plasmid or miR200c agomir not only meaningfully inhibited EMT of melanoma, reduced the EMT characteristic molecular expression in tumor tissues, but also significantly decreased the Treg cells and TGF-β1, enhanced the cytotoxicities of NK cells and cytotoxic T lymphocytes and the IFN-γ level. Furthermore, the immunotherapeutic combination resulted in inhibiting the melanoma growth and lung metastasis. Our study demonstrated that using the B16F10/GPI-IL-21 vaccine in combination with the down-regulated ZEB1 or miR200c administration effectively elicited anti-tumor immunity and reduced melanoma metastasis by inhibiting the EMT program in the B16F10 melanoma-bearing mice. PMID:27133050

  17. 50 CFR 300.200 - Purpose and scope.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Identification and Certification of Nations § 300.200 Purpose and scope. The purpose of this subpart is to... Protection Act”) to identify and certify nations whose vessels are engaged in illegal, unreported, or... language applies to vessels entitled to fly the flag of the nation in question. Identified nations that...

  18. PLOT300: A Tektronics PLOT10 emulator for HP 9000 series 200/300 computers

    NASA Technical Reports Server (NTRS)

    Kjelgaard, Scott O.

    1988-01-01

    A software package which emulates the Tektronics PLOT10 Graphics package on Hewlett-Packard 9000 Series 200/300 computers is described. The Software is written in HP Rocky Mountain BASIC and can be run under BASIC revisions 3.0 and 4.0. Although this subroutine library emulates a subset of PLOT10, several subroutines have been added which enhance basic plot generation. Example codes using PLOT300 and descriptions of the subroutines are included in the text.

  19. Fluid-Rock Interactions at the Interface between Reservoir Rock and Cap Rock: An Experimental Case Study Regarding Mineral Trapping at 54 and 200 C

    NASA Astrophysics Data System (ADS)

    Wigand, M. O.; Carey, J. W.; Kaszuba, J. P.; Hollis, W. K.

    2006-12-01

    Geologic sequestration (underground storage) of carbon dioxide (CO2) is the most feasible approach to mitigating CO2-induced global warming while maintaining the current fossil fuel-based economy. Although simple in principle, effective implementation of geologic sequestration will require significant development of the scientific understanding of interactions among injected CO2 (as a supercritical fluid), brine, and the reservoir rock. This paper presents the results of flow-through experiments that simulated a rising plume of supercritical carbon dioxide (SCCO2) interacting at the interface between reservoir rock and cap rock in a brine- saturated aquifer. We performed two high pressure flow-through experiments using powdered limestone and illite-rich shale separated by a frit with a pore size of 10 μm. One experiment was performed under in-situ pressure (2880 psi) and temperature (54°C) conditions of a typical oil reservoir in the Permian Basin. To increase the kinetic rates of the mineral reactions we also performed an experiment at elevated temperatures (200°C) but using the same boundary conditions for other experimental parameters such as pressure, rock samples, fluids, injection rates, and time span. Both experiments were performed over 3263 hours. After the experiment eight disks of equal size representing different reaction zones were cut to investigate the progressive fluid-rock interaction of the reservoir and cap rock with the mixture of SCCO2 and brine. Additionally, fluid samples were frequently collected and their compositions were analyzed using inductively coupled plasma mass spectrometry. At in-situ temperature conditions we determined an enrichment of B, Ba, Cr, Cu, Fe, K, Li, Mg, Na, Ni, Rb, Si, Sr, Ti, Zn, and sulfate in the collected brines, whereas Mn and chloride concentrations were depleted in comparison with the starting composition. Ca and Al showed uneven behavior with changes in enrichment and depletion during the experiment. At 200

  20. Heterogeneous expression of zinc-finger E-box-binding homeobox 1 plays a pivotal role in metastasis via regulation of miR-200c in epithelial-mesenchymal transition.

    PubMed

    Muto, Yuta; Suzuki, Koichi; Kato, Takaharu; Tsujinaka, Shingo; Ichida, Kosuke; Takayama, Yuji; Fukui, Taro; Kakizawa, Nao; Watanabe, Fumiaki; Saito, Masaaki; Futsuhara, Kazushige; Noda, Hiroshi; Miyakura, Yasuyuki; Konishi, Fumio; Rikiyama, Toshiki

    2016-09-01

    Although epithelial-mesenchymal transition (EMT) has been implicated as the pivotal event in metastasis, there is insufficient evidence related to EMT in clinical settings. Intratumor heterogeneity may lead to underestimation of gene expression representing EMT. In the present study, we investigated the expression of EMT-associated genes and microRNAs in primary colorectal cancer while considering intratumor heterogeneity. One-hundred and thirty-three multiple spatially separated samples were obtained from 8 patients with metastatic colorectal cancers and 8 with non-metastatic colorectal cancers, from the tumor center (TC), invasive front (IF) and metastasis. Differences in gene and microRNA expression were investigated by microarray and quantitative reverse-transcription PCR. Gene expression microarray analysis detected 7920 sites showing differing levels of gene expression among the TC, IF and metastasis. Expression of the EMT-associated gene zinc-finger E-box-binding homeobox 1 (ZEB1) significantly increased in the IF (p<0.01). To exclude individual differences, the expression ratio between TC and IF in each tumor was applied to analysis. This approach enabled recognition of the activation of the VEGF and Wnt signaling pathways, which were involved in metastasis via promotion of EMT. While no activation of these pathways was seen at the TC, regardless of whether tumors were metastatic or non-metastatic, they were preferentially activated at the IF in metastatic tumors, where high ZEB1 expression was seen in connection with decreased miR-200c expression. Multiple sampling in a tumor revealed that heterogeneous ZEB1 expression induced by EMT-associated signaling pathways played a pivotal role in metastasis via regulation of miR-200c. PMID:27315529

  1. 78 FR 70848 - Special Conditions: Boeing Model 777-200, -300, and -300ER Series Airplanes; Aircraft Electronic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-27

    ... the EFB architecture and existing airplane network systems. The applicable airworthiness regulations... interface to existing aircraft systems. The proposed network architecture is used for a diverse set of... architecture and network configuration in the Boeing Model 777- 200, -300, and -300ER series airplanes...

  2. 76 FR 68366 - Airworthiness Directives; The Boeing Company Model 777-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-04

    ... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979), (3) Will... Directives; The Boeing Company Model 777-200 and - 300 Series Airplanes AGENCY: Federal Aviation... airworthiness directive (AD) for certain The Boeing Company Model 777-200 and -300 series airplanes....

  3. 24 CFR 200.300 - Nondiscrimination and fair housing policy.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... administered in accordance with: (a) The nondiscrimination and fair housing requirements set forth in 24 CFR... forth in 24 CFR 5.105(a)(2); and (b) The affirmative fair housing marketing requirements in 24 CFR part 200, subpart M and 24 CFR part 108....

  4. 24 CFR 200.300 - Nondiscrimination and fair housing policy.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... administered in accordance with: (a) The nondiscrimination and fair housing requirements set forth in 24 CFR... forth in 24 CFR 5.105(a)(2); and (b) The affirmative fair housing marketing requirements in 24 CFR part 200, subpart M and 24 CFR part 108....

  5. 24 CFR 200.300 - Nondiscrimination and fair housing policy.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... administered in accordance with: (a) The nondiscrimination and fair housing requirements set forth in 24 CFR... forth in 24 CFR 5.105(a)(2); and (b) The affirmative fair housing marketing requirements in 24 CFR part 200, subpart M and 24 CFR part 108....

  6. 76 FR 78138 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-16

    ..., Amendment 39-16531 (75 FR 78588, December 16, 2010) (g) Corrective Actions (Installing Teflon Sleeving, Cap... (75 FR 78588, December 16, 2010). We must receive any comments on this AD by January 30, 2012... AD 2010-24-12, amendment 39-16531 (75 FR 78588, December 16, 2010), for certain Model 777-200,...

  7. 77 FR 26663 - Airworthiness Directives; The Boeing Company Model 767-200, -300, -300F, and -400ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-07

    ...We are superseding an existing airworthiness directive (AD) that applies to The Boeing Company Model 767-200, -300, and -300F series airplanes. That AD currently requires inspections to detect cracking or corrosion of the fail-safe straps between the side fitting of the rear spar bulkhead at body station 955 and the skin; and follow- on and corrective actions. This new AD expands the......

  8. Environmental assessment for the salvage/demolition of 200 West Area, 200 East Area, and 300 Area steam plants

    SciTech Connect

    1996-10-01

    This environmental assessment has been prepared to assess potential environmental impacts associated with the US Department of Energy`s proposed action: the salvage/demolition of the 200 West Area, 200 East Area, and 300 Area Steam Plants and steam distribution piping. Impact information will be used by the US Department of Energy, Richland Operations Office Manager, to determine if the proposed action is a major federal action significantly affecting the quality of the human environment. If the proposed action is determined to be major and significant, an environmental impact statement will be prepared. If the proposed action is determined not to be major and significant, a Finding of No Significant Impact (FONSI) will be issued and the action can proceed. The proposed action involves the salvage and demolition of the 200 West Area, 200 East Are, and 300 Area steam plants and their associated steam distribution piping, equipment, and ancillary facilities. Activities include the salvaging and recycling of all materials, wastes, and equipment where feasible, with waste minimization efforts utilized.

  9. 76 FR 53305 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-26

    ... (76 FR 8610, February 15, 2011), addresses Model A340-200, -300, -500, and -600 series airplanes. (2...-211-AD; Amendment 39-16773; AD 2011-17-09] RIN 2120-AA64 Airworthiness Directives; Airbus Model A330... reference of a certain other publication listed in this AD as of June 7, 2006 (71 FR 25919, May 3,...

  10. 76 FR 15867 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ...-09-07, Amendment 39-14577 (71 FR 25919, May 3, 2006). That AD requires actions intended to address an... 39-16605 (76 FR 8612, February 15, 2011), for Airbus Model A340-200, -300, -500, and -600 series... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR...

  11. 76 FR 15872 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-22

    ... issued AD 2006-09-07, Amendment 39-14577 (71 FR 25919, May 3, 2006), that applies to Airbus Model A330... FR 8610, February 15, 2011), for Airbus Model A340-200, -300, -500, and -600 series airplanes, to... rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3....

  12. The system water-sodium oxide-silicon dioxide at 200, 250, and 300°

    USGS Publications Warehouse

    Rowe, Jack J.; Fournier, Robert O.; Morey, G.W.

    1967-01-01

    Studies were made of the H2O-Na2O-SiO2 system at its vapor pressure at 200, 250, and 300??. Three different sodium trisilicate hydrates were encountered in the investigation. At 300??, Na2Si3O7??5H2O is found: at 250??, Na2Si3O7??6H2O; and at 200??, Na2Si3O7??11H2O. The liquid immiscibility previously reported to exist in the system was found to be a quenching phenomenon caused by the decomposition of the hydrates to unstable, supersaturated, viscous liquids. Under conditions where equilibrium is maintained, as temperature is lowered, the hydrates decompose to quartz, sodium disilicate, and liquid. The retrograde solubility of sodium disilicate and its tendency to form supersaturated solutions during heating from 25 to 250?? account for higher solubilities reported by others than were found in this study. The solubility of sodium disilicate in water is 26% at 200??, 9% at 250??, and 5% at 300??. Sodium metasilicate solubility is 38% at 200?? and 34% at 250??; this compound is incongruently soluble at 300??.

  13. 75 FR 47207 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes, and Model A340-200...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-05

    ...- 19, Amendment 39-16364 (75 FR 39814, July 13, 2010), for certain Airbus Model A330-200 and -300... A340-200, -300, -500 and -600 series airplanes. A certain service bulletin number in Note 3 of the regulatory section is incorrect. This document corrects that service bulletin number. In all other...

  14. 75 FR 60010 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes and Model A340-200...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-29

    ... rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will... Directives; Airbus Model A330-200 and -300 Series Airplanes and Model A340-200, -300, -500, and -600 Series... connector internal arcing and hydraulic system malfunction. In addition, as the connectors are located...

  15. 76 FR 69685 - Airworthiness Directives; The Boeing Company Model 767-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-09

    ...We propose to adopt a new airworthiness directive (AD) for all The Boeing Company Model 767-200 and -300 series airplanes. This proposed AD was prompted by reports of cracks in the inner chords at both left-side and right-side stations 859.5, 883.5, and 903.5. This proposed AD would require repetitive inspections of the frame inner chord transition radius for cracks, and related investigative......

  16. 75 FR 28475 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes, and Model A340-300...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-21

    ...-122-AD; Amendment 39-16304; AD 2010-10-25] RIN 2120-AA64 Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes, and Model A340-300 Series Airplanes AGENCY: Federal Aviation... products. That NPRM was published in the Federal Register on October 19, 2009 (74 FR 53440). That...

  17. Thermal quenching of thermoluminescence in TLD-200, TLD-300 and TLD-400 after β-irradiation

    NASA Astrophysics Data System (ADS)

    Kafadar, Vural E.

    2011-02-01

    The dosimetric characteristics of many TL materials are influenced by changes in location, size and shape of the glow curves due to changes in the heating rate. In this study, the effect of heating rate on the integrated peak areas of CaF 2:Dy (TLD-200), CaF 2:Tm (TLD-300) and CaF 2:Mn (TLD-400) crystals have been investigated after β-irradiation. It was observed that the peak temperatures of all peaks shifted to the high temperature sides and the integrated peak areas decrease as the heating rate increases due to thermal quenching, whose efficiency increases as temperature increases.

  18. 75 FR 9515 - Airworthiness Directives; Airbus Model A330-200 Series Airplanes and Model A340-200 and -300...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-03

    ...-16202; AD 2010-04-09] RIN 2120-AA64 Airworthiness Directives; Airbus Model A330-200 Series Airplanes and Model A340-200 and -300 Series Airplanes Correction In rule document 2010-3119 beginning on page 7940 in..., under the header ``Applicability,'' item (1) should read: ``(1) Airbus Model A330-201, - 202, -203,...

  19. 75 FR 7940 - Airworthiness Directives; Airbus Model A330-200 Series Airplanes and Model A340-200 and -300...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-23

    ...-138-AD; Amendment 39-16202; AD 2010-04-09] RIN 2120-AA64 Airworthiness Directives; Airbus Model A330-200 Series Airplanes and Model A340-200 and -300 Series Airplanes AGENCY: Federal Aviation... products. That NPRM was published in the Federal Register on December 1, 2009 (74 FR 62713). That...

  20. 76 FR 30253 - Airworthiness Directives; The Boeing Company Model 747-100, 747-100B, 747-100B SUD, 747-200B, 747...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-25

    ...-16532 (75 FR 78591, ] December 16, 2010), currently requires adding two new indicator lights on a... Model 747-100, 747- 100B, 747-100B SUD, 747-200B, 747-200C, 747-200F, 747-300, 747SR, and 747SP Series... requires those actions for Model 747-100, 747-100B, 747-100B SUD, 747-200B, 747-200C, 747-200F,...

  1. Experimental study of a megawatt 200--300 GHz gyrotron oscillator

    SciTech Connect

    Grimm, T.L.; Kreischer, K.E.; Temkin, R.J. )

    1993-11-01

    A detailed experimental study is presented of a pulsed megawatt gyrotron oscillator operating in the 200--300 GHz range, whose design is consistent with continuous operation for electron cyclotron resonance heating (ECRH) of fusion plasmas. Two different radii beams produced by magnetron injection guns (MIG's) were used to excite the cylindrical waveguide cavity. The emission was found experimentally to be single mode, single frequency with a single rotation, which can be mode converted for transmission. The highest power reached with the larger radius electron beam was 1.2 MW at 230 GHz in the TE[sub 34,6] mode with an efficiency of 20% and beam parameters of 59 A and 100 kV. The highest power reached with the smaller radius electron beam was 0.78 MW at 280 GHz in the TE[sub 25,13] mode with an efficiency of 17% and beam parameters of 51 A and 92 kV. The smaller radius beam gave a peak efficiency of 18% at 0.72 MW, 290 GHz in the TE[sub 25,14] mode. Efficiencies obtained in this experiment are about half that of less highly overmoded gyrotrons. Analysis of the experiment suggests that the low efficiency is primarily caused by azimuthal mode competition, in agreement with multimode theory for a tapered cavity. These experimental results show that megawatt power levels can be generated in continuous wave (cw) gyrotron oscillators at 200--300 GHz with efficiencies approaching 20%.

  2. 78 FR 76731 - Special Conditions: Boeing Model 777-200, -300, and -300ER Series Airplanes; Rechargeable Lithium...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... battery systems special conditions adopted for the Boeing Model 787 (72 FR 57842; October 11, 2007..., -300, and -300ER series airplanes, was published in the Federal Register on August 22, 2013 (78 FR... Series Airplanes; Rechargeable Lithium Ion Batteries and Battery Systems AGENCY: Federal...

  3. 75 FR 15322 - Airworthiness Directives; The Boeing Company Model 767-200, -300, and -300F Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... series airplanes. That NPRM was published in the Federal Register on July 2, 2009 (74 FR 31640). For...'' under DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979), and (3) Will not have a..., -300, and -300F series airplanes. For certain airplanes, this AD requires installing support...

  4. 76 FR 61645 - Airworthiness Directives; Airbus Model A330-200 Series Airplanes; Model A330-300 Series Airplanes...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-05

    ... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Airbus Model A330-200 Series Airplanes; Model A330-300 Series Airplanes; Model A340-200 Series Airplanes; and Model A340... November 21, 2011. ADDRESSES: You may send comments by any of the following methods: Federal...

  5. 76 FR 4219 - Airworthiness Directives; Airbus Model A330-200 Series Airplanes; Model A330-300 Series Airplanes...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-25

    ...-279-AD; Amendment 39-16583; AD 2011-02-09] RIN 2120-AA64 Airworthiness Directives; Airbus Model A330-200 Series Airplanes; Model A330-300 Series Airplanes; Model A340-200 Series Airplanes; and Model A340... comments on this AD by March 11, 2011. ADDRESSES: You may send comments by any of the following...

  6. 78 FR 68985 - Special Conditions: Boeing Model 777-200, -300, and -300ER Series Airplanes; Aircraft Electronic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-18

    ... and data networks. This onboard network system will be composed of a network file server, a network... installation of an onboard network system, associated line replaceable units (LRUs) and additional software..., -300ER series airplanes will incorporate the following novel or unusual design features: An...

  7. 75 FR 64633 - Airworthiness Directives; The Boeing Company Model 767-200, -300, and -300F Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-20

    ... publication listed in this AD as of November 5, 2009 (74 FR 50692, October 1, 2009). We must receive comments..., we issued AD 2009-20-09, Amendment 39-16032 (74 FR 50692, October 1, 2009), for certain Model 767-200... this AD terminate certain requirements of AD 2000-19-09, Amendment 39-11910 (65 FR 58641, October...

  8. Cell spreading on titanium periodic nanostructures with periods of 200, 300 and 600 nm produced by femtosecond laser irradiation

    NASA Astrophysics Data System (ADS)

    Tsukamoto, M.; Kawa, T.; Shinonaga, T.; Chen, P.; Nagai, A.; Hanawa, T.

    2016-02-01

    Titanium (Ti) is an important biomaterial. We have used femtosecond laser irradiation to form periodic nanostructures on Ti plate for control of the cell spreading. In this study, periodic nanostructures with periodicities of 200, 300 and 600 nm were formed on a Ti plates using a femtosecond laser with wavelengths of 258, 388 and 775 nm, respectively. Cell spreading on the Ti plate for periodic nanostructures with periodicity of 200 nm lacked a definite direction, whereas cell spreadings on the Ti plate for periodic nanostructures with periodicities of 300 and 600 nm occurred along the grooves.

  9. 76 FR 8612 - Airworthiness Directives; Airbus Model A340-200, -300, -500, and -600 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-15

    ... 2006-09-07, Amendment 39-14577 (71 FR 25919, May 3, 2006), for all Airbus Model A330-200 and -300, and...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have.... Adapted airworthiness limitations were introduced in Airbus A340 Airworthiness Limitations Section...

  10. 76 FR 42607 - Airworthiness Directives; The Boeing Company Model 767-200, -300, and -400ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-19

    ...-15818 (74 FR 8717, February 26, 2009), for certain Model 767-200, -300, and - 400ER series airplanes...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979), (3) Will not affect... FR 8717, February 26, 2009), and adding the following new AD: The Boeing Company: Docket No....

  11. N200 and P300 as orthogonal and integrable indicators of distinct awareness and recognition processes in memory detection.

    PubMed

    Hu, Xiaoqing; Pornpattananangkul, Narun; Rosenfeld, J Peter

    2013-05-01

    In an event-related potential (ERP)-based concealed information test (CIT), we investigated the effect of manipulated awareness of concealed information on the ERPs. Participants either committed a mock crime or not (guilty vs. innocent) before the CIT, and received feedback regarding either specific (high awareness) or general (low awareness) task performance during the CIT. We found that awareness and recognition of the crime-relevant information differentially influenced the frontal-central N200 and parietal P300: Probe elicited a larger N200 than irrelevant only when guilty participants were in the high awareness condition, whereas the P300 was mainly responsive to information recognition. No N200-P300 correlation was found, allowing for a combined measure of both yielding the highest detection efficiency in the high awareness group (AUC = .91). Finally, a color-naming Stroop task following the CIT revealed that guilty participants showed larger interference effects than innocent participants, suggesting that the former expended more attentional resources during the CIT. PMID:23317115

  12. Life Test Results for Water Heat Pipes Operating at 200 °C to 300 °C

    NASA Astrophysics Data System (ADS)

    Rosenfeld, John H.; Gernert, Nelson J.

    2008-01-01

    For lunar or planetary bases to be viable, a robust electric generating system will be required for powering the habitat. Water heat pipes offer an attractive solution for lunar base heat rejection, and would serve as a qualification for them on other long duration missions. Successful operation near the upper end of water operating range is a requirement for the application. Results are reported for life tests on water heat pipes that were operated at various temperatures between 200 °C and 300 °C. Tests were conducted on twenty three gravity-assisted water heat pipes. Eleven titanium/water heat pipes and ten Monel/water heat pipes were tested at temperatures above 200 °C. Two cupronickel heat pipes were also assembled and tested. Titanium alloys tested included CP-2 titanium, as well as two beta-titanium alloys, namely 15-3 and Nitinol alloys. Some of the titanium alloy life tests used wicks fabricated from CP-2 titanium screen or porous felt. Monel alloys tested included 400 and K-500 alloys. Some of the Monel heat pipes contained copper/nickel wicks that were fabricated by brazing nickel-plated copper felt metal wicks. Although most of the envelope/material combinations exhibit favorable results at 200 °C, some of the combinations failed at higher temperatures. Causes of failure included stress-creep of envelopes and corrosion at axial or end cap welds. This information represents a significant advance in selection of materials for 200 °C to 300 °C water heat pipes. Life testing work is being continued.

  13. Phenotypes and Virulence among Staphylococcus aureus USA100, USA200, USA300, USA400, and USA600 Clonal Lineages

    PubMed Central

    King, Jessica M.; Kulhankova, Katarina; Stach, Christopher S.; Vu, Bao G.

    2016-01-01

    ABSTRACT Staphylococcus aureus diseases affect ~500,000 individuals per year in the United States. Worldwide, the USA100, USA200, USA400, and USA600 lineages cause many of the life-threatening S. aureus infections, such as bacteremia, infective endocarditis, pneumonia, toxic shock syndrome, and surgical site infections. However, the virulence mechanisms associated with these clonal lineages, in particular the USA100 and USA600 isolates, have been severely understudied. We investigated the virulence of these strains, in addition to strains in the USA200, USA300, and USA400 types, in well-established in vitro assays and in vivo in the rabbit model of infective endocarditis and sepsis. We show in the infective endocarditis and sepsis model that strains in the USA100 and USA600 lineages cause high lethality and are proficient in causing native valve infective endocarditis. Strains with high cytolytic activity or producing toxic shock syndrome toxin 1 (TSST-1) or staphylococcal enterotoxin C (SEC) caused lethal sepsis, even with low cytolytic activity. Strains in the USA100, USA200, USA400, and USA600 lineages consistently contained genes that encode for the enterotoxin gene cluster proteins, SEC, or TSST-1 and were proficient at causing infective endocarditis, while the USA300 strains lacked these toxins and were deficient in promoting vegetation growth. The USA100, USA200, and USA400 strains in our collection formed strong biofilms in vitro, whereas the USA200 and USA600 strains exhibited increased blood survival. Hence, infective endocarditis and lethal sepsis are multifactorial and not intrinsic to any one individual clonal group, further highlighting the importance of expanding our knowledge of S. aureus pathogenesis to clonal lineages causative of invasive disease. IMPORTANCE S. aureus is the leading cause of infective endocarditis in the developed world, affecting ~40,000 individuals each year in the United States, and the second leading cause of bacteremia (D

  14. Phenotypes and Virulence among Staphylococcus aureus USA100, USA200, USA300, USA400, and USA600 Clonal Lineages.

    PubMed

    King, Jessica M; Kulhankova, Katarina; Stach, Christopher S; Vu, Bao G; Salgado-Pabón, Wilmara

    2016-01-01

    Staphylococcus aureus diseases affect ~500,000 individuals per year in the United States. Worldwide, the USA100, USA200, USA400, and USA600 lineages cause many of the life-threatening S. aureus infections, such as bacteremia, infective endocarditis, pneumonia, toxic shock syndrome, and surgical site infections. However, the virulence mechanisms associated with these clonal lineages, in particular the USA100 and USA600 isolates, have been severely understudied. We investigated the virulence of these strains, in addition to strains in the USA200, USA300, and USA400 types, in well-established in vitro assays and in vivo in the rabbit model of infective endocarditis and sepsis. We show in the infective endocarditis and sepsis model that strains in the USA100 and USA600 lineages cause high lethality and are proficient in causing native valve infective endocarditis. Strains with high cytolytic activity or producing toxic shock syndrome toxin 1 (TSST-1) or staphylococcal enterotoxin C (SEC) caused lethal sepsis, even with low cytolytic activity. Strains in the USA100, USA200, USA400, and USA600 lineages consistently contained genes that encode for the enterotoxin gene cluster proteins, SEC, or TSST-1 and were proficient at causing infective endocarditis, while the USA300 strains lacked these toxins and were deficient in promoting vegetation growth. The USA100, USA200, and USA400 strains in our collection formed strong biofilms in vitro, whereas the USA200 and USA600 strains exhibited increased blood survival. Hence, infective endocarditis and lethal sepsis are multifactorial and not intrinsic to any one individual clonal group, further highlighting the importance of expanding our knowledge of S. aureus pathogenesis to clonal lineages causative of invasive disease. IMPORTANCE S. aureus is the leading cause of infective endocarditis in the developed world, affecting ~40,000 individuals each year in the United States, and the second leading cause of bacteremia (D. R

  15. 76 FR 36011 - Airworthiness Directives; Fokker Services B.V. Model F.27 Mark 050, 200, 300, 400, 500, 600, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-21

    ...'' (66 FR 23086, May 7, 2001). In addition to new airworthiness standards for transport airplanes and new... rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will.... Model F.27 Mark 050, 200, 300, 400, 500, 600, and 700 Airplanes; and Model F.28 Airplanes...

  16. 75 FR 7949 - Airworthiness Directives; Extra Flugzeugproduktions- und Vertriebs- GmbH Models EA-300/200 and EA...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-23

    ... Register on November 3, 2009 (74 FR 56748). That NPRM proposed to correct an unsafe condition for the... Procedures (44 FR 11034, February 26, 1979); and (3) Will not have a significant economic impact, positive or... through 1043; and (2) Model EA-300/L airplanes, S/N 01 through 170, 172, 173, 1171, and 1174 through...

  17. Geochemical Controls on Contaminant Uranium in Vadose Hanford Formation Sediments at the 200 Area and 300 Area, Hanford Site, Washington

    SciTech Connect

    McKinley, James P.; Zachara, John M.; Wan, Jiamin; Mccready, David E.; Heald, Steve M.

    2007-11-01

    differences in uranium contributed by contaminated vadose sediments at two locations was investigated. At the BX tank farms, alkaline waste was accidentally released to a thick vadose zone. At the 300 Area, waste of variable acidity was released by unintended infiltration through the base of settling ponds. The waste form at the BX site was devoid of dissolved silica, and reacted with fluids trapped in microfractures to precipitate uranyl silicates. These secondary deposits were isolated physically from the vadose pore space and are not readily leached into pore fluids. At the 300 Area, the aluminum-rich waste precipitated on the surfaces of sediment clasts, forming a microporous reservoir of solid-phase uranium. Interaction of this coating with water in transit through the vadose zone provides a persistent source of dissolved uranium to groundwater.

  18. Effect of high-pressure treatment of ewe raw milk curd at 200 and 300 MPa on characteristics of Hispánico cheese.

    PubMed

    Alonso, R; Picon, A; Gaya, P; Fernández-García, E; Nuñez, M

    2012-07-01

    Hispánico cheese is a semihard variety made from a mixture of cow and ewe milks. Production of ewe milk declines in summer and autumn. To surmount the seasonal shortage of ewe milk and prevent the inactivation of milk enzymes by pasteurization, curd made in spring from ewe raw milk was pressurized at 200 and 300 MPa and stored frozen for 4 mo. Thawed ewe milk curds were added to fresh curd made from pasteurized cow milk for the manufacture of experimental Hispánico cheeses. Control cheese was made from a mixture of pasteurized cow and ewe milk in the same proportions as those used for experimental cheeses. Experimental cheeses exhibited lower dry matter content, higher aminopeptidase activity and total free amino acid concentration, and higher levels of acetic and propionic acids, aldehydes, alcohols, and esters compared with control cheese. In contrast, the concentration of total free fatty acids and ketones and the levels of textural parameters were significantly higher in control cheese. The use of ewe raw milk curd pressurized at 200 and 300 MPa, stored frozen and thawed for Hispánico cheese manufacture, was generally beneficial for cheese characteristics and increased cheese yield because of the lower dry matter content of experimental cheeses. PMID:22720909

  19. 75 FR 16651 - Airworthiness Directives; The Boeing Company Model 747-200C and -200F Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-02

    ... supersedes AD 2006-08-02, amendment 39-14556 (70 FR 18618, April 12, 2006). The existing AD applies to... August 12, 2009 (74 FR 40529). That NPRM proposed to continue to require repetitive inspections to find...'' under DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and (3) Will not have...

  20. 75 FR 5692 - Airworthiness Directives; The Boeing Company Model 747-200C and -200F Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-04

    ... published in the Federal Register on July 6, 2009 (74 FR 31894). That NPRM proposed to require a high... 2005- 07-21, Amendment 39-14046 (70 FR 18277, April 11, 2005), is required during the accomplishment of... alternative method of compliance (AMOC) for paragraphs (f) and (g) of AD 2006-08-02, Amendment 39-14556 (71...

  1. Aggregate sound velocities and acoustic Grüneisen parameter of iron up to 300 GPa and 1,200 K

    PubMed Central

    Dubrovinsky, L. S.; Dubrovinskaia, N. A.; Le Bihan, T.

    2001-01-01

    Successful interpretation of available geophysical data requires experimental and theoretical information on the elasticity of solids under physical conditions of Earth's interior. Because iron is considered as major component in Earth's core, elastic properties of iron at high pressures and temperatures are very important for modeling its composition and dynamics. We use in situ x-ray diffraction data on ɛ-iron at static pressures up to 300 GPa and temperatures to 1,200 K to determine the Debye–Waller temperature factors and calculate aggregate sound velocities and Grüneisen parameter of ɛ-iron by using an approach that is based on Rietveld refinement at high pressures and temperatures. PMID:11504937

  2. A room-temperature phase transition in maximum microcline. Absorption in the far infrared (10 200 cm-1) in the temperature range 110 300 K

    NASA Astrophysics Data System (ADS)

    Wyncke, B.; McMillan, P. F.; Brown, W. L.; Openshaw, R. E.; Bréhat, F.

    1981-02-01

    The far infrared powder absorption spectra (10 200 cm-1) for a maximum microcline sample (obtained by ion-exchange from Amelia albite) and for a natural maximum microcline were measured at steps of 10 K on lowering and raising the temperature between 300 and 110 K. Of the absorption bands at 97.5, 113.5, 137.5, 148, and 157 cm-1 occurring at room temperature, those at 157 and eventually 148 cm-1 showed evidence of a phase transition and only that at 157 cm-1 showed hysteresis. The transition occurs at 245±5 K on lowering the temperature and the low-temperature form can be superheated to 300 K in the case of the ion-exchange sample. Oriented thin sections parallel to (001) and (010) were also measured to 110 K as a function of the direction of the electric vector E, but could not be unambiguously interpreted. Though the effect is very slight it is tentatively proposed that the phase transition (already characterized by calorimetry and lattice parameters) involves changes in K-O and T-O-T bonds.

  3. Novel spin-coating technology for 248-nm/193-nm DUV lithography and low-k spin on dielectrics of 200-mm/300-mm wafers

    NASA Astrophysics Data System (ADS)

    Gurer, Emir; Zhong, Tom X.; Lewellen, John W.; Lee, Ed C.

    2000-06-01

    An alternative coating technology was developed for 248 nm/193 nm DUV lithography and low-k spin on dielectric (SOD) materials used in the interconnect area. This is a 300 mm enabling technology which overcomes turbulent flow limitations above 2000 rpm and it prevents 40 - 60% reduction on the process latitudes of evaporation-related variables, common to 300 mm conventional coaters. Our new coating technology is fully enclosed and it is capable of controlling the solvent concentration above the resist film dynamically in the gas phase. This feature allows a direct control of the evaporation mass transfer which determines the quality of the final resist profiles. Following process advantages are reported in this paper: (1) Demonstrated that final resist film thickness can be routinely varied by 4000 angstrom at a fixed drying spin speed, thus minimizing the impact of turbulence wall for 300 mm wafers. (2) Evaporation control allows wider range of useful thickness from a fixed viscosity material. (3) Latitudes of evaporation-related process variables is about 40% larger than that of a conventional coater. (4) Highly uniform films of 0.05% were obtained for 8800 angstrom target thickness with tighter wafer-wafer profile control because of the enclosed nature of the technology. (5) Dynamic evaporation control facilitates resist consumption minimization. Preliminary results indicate feasibility of a 0.4 cc process of record (POR) for a 200 mm substrate. (6) Lower COO due to demonstrated relative insensitivity to environmental variables, robust resist consumption minimization and superior process capabilities. (7) Improved planarization and gap fill properties for the new generation photoresist/low-k SOD materials deposited using this enclosed coating technology.

  4. The impact of low Al-content waveguides on power and efficiency of 9xx nm diode lasers between 200 and 300 K

    NASA Astrophysics Data System (ADS)

    Frevert, C.; Crump, P.; Bugge, F.; Knigge, S.; Erbert, G.

    2016-02-01

    We present results on investigations of 9xx nm, GaAs-based diode lasers with 100 μm wide, 4 mm long stripes operating at temperatures between 200 and 300 K. As temperatures are reduced to 218 K, efficiency and power are improved. We analyze the characteristic parameters and subsequently mitigate the limiting factors by altering the vertical epitaxial design, seeking to further increase the optical output power and efficiency. The temperature dependence of internal parameters is obtained from length-dependent measurements, showing that the improved performance is due to an increased differential internal efficiency. Series resistance is confirmed as the main remaining limit to higher efficiency at high power. We show that the series resistance can be reduced by lowering the aluminum content in the Al x Ga1-x As waveguide which increases the carrier mobility. Although poor optical performance is seen at room temperature in low Al-content structures due to carrier leakage, at 218 K this is suppressed and is accompanied by significantly reduced series resistance. However, at high powers we observe further (non-thermal) power saturation in low Al-content structures even at 208 K, which limits the efficiency gain at high powers. Analysis of the series resistance of laser structures with different waveguide compositions at temperatures between 208 and 298 K reveals that series resistance is a function of the barrier height around the quantum well. This is taken as first evidence for carrier transport being a significant limit to electrical resistance. Finally, structures with an optimized Al-content are shown to maintain conversion efficiency >65% to output powers of 20 W, substantially higher than previously reported at 300 K (˜55% at 20 W).

  5. The ion-exchange capacity and Cs, Sr leachability of natural clinoptilolites at 200--300 C and PH{sub 2}O{sub sat}

    SciTech Connect

    Redkin, A.F.; Hemley, J.J.; Doughten, M.W.; Cygan, G.L.

    1995-12-31

    Clinoptilolites of three US clinoptilolite-rich tuff deposits, with different alkali and alkaline earth element mole ratios, were used for experimental investigation. The runs on stability and sorption ability were carried out in water and 0.3 molal chloride solution, using individual minerals and using assemblages containing microcline and analcime at 200 to 300 C and PH{sub 2}O{sub sat} water (duration of one month and two weeks). The clinoptilolites showed different behavior, unrelated to exchange capacity, when run under similar conditions. It was also found that Cs-bearing clinoptilolites contained 12--14 wt.% Cs after runs in 0.1mNaCl+0.1mKCl+ 0.1mCsCl, whereas Sr-bearing clinoptilolite contained about 3--5% Sr in 0.1mNaCl+0.1mKCl+0.05mSrCl{sub 2}. The compositional trend showed some linear variation between Sr and Cs content and Si/Al mole ratio up to Si/Al=5.0. Thermal investigation showed that (Na-K)rich clinoptilolite completely decomposes at 325 C, (Na-Mg) rich at 350 C and (Ca-Mg) rich at 375 C in runs of 2 weeks duration in pure water. Na-analcimes associated with clinoptilolites have low Cs and Sr adsorption capacity relative to the clinoptilolites. Na-Sr -bearing analcimes have an inverse dependence of Sr/(Sr+Na+K) mole ratio with Si/Al in the reaction of heterovalent exchange in analcime, where Na and Si replace Sr and Al. Thermodynamic calculation was done for the solubility of Cs and Sr-bearing clinoptilolites at 300 C, PH{sub 2}O{sub sat}. It was established that under some conditions Sr leachability from Sr-bearing clinoptilolite is insignificant whereas Cs-bearing clinoptilolite leachability is considerably higher.

  6. Formation of CO2, H2 and condensed carbon from siderite dissolution in the 200-300 °C range and at 50 MPa

    NASA Astrophysics Data System (ADS)

    Milesi, Vincent; Guyot, François; Brunet, Fabrice; Richard, Laurent; Recham, Nadir; Benedetti, Marc; Dairou, Julien; Prinzhofer, Alain

    2015-04-01

    Laboratory experiments were conducted to investigate the chemical processes governing the carbon speciation associated to hydrothermal decomposition of siderite. Experiments were carried out in sealed gold capsules using synthetic siderite and deionised water. The samples were reacted at 200 and 300 °C, under a pressure of 50 MPa. Siderite dissolved to reach the 3FeCO3 + H2O = Fe3O4 + 3CO2 + H2 equilibrium and magnetite, Fe3O4, was produced accordingly. The gas phase was dominated by CO2, H2 and CH4, the latter being in strong thermodynamic disequilibrium with CO2. Contrary to the other gas products, H2 concentration was found to decrease with run duration. TEM observations showed the occurrence of condensed carbon phases at the surfaces of magnetite and residual siderite grains. Thermodynamic calculations predict the formation of condensed carbon in the experiments according to the reaction: CO2 + 2H2 ⇒ C + 2H2O, which accounted for the observed H2 concentration decrease up to the point where H2 and CO2 activities were buffered by the graphite-siderite-magnetite assemblage. The well-organized structure of the carbon coating around magnetite emphasizes the high catalytic potential of magnetite surface for carbon reduction and polymerization. The formation of such C-rich phases may represent a potential source of CH4 by hydrogenation. On the other hand, the catalysis of Fischer-Tropsch type reactions may be poisoned by the presence of carbon coating on mineral surfaces. In any case, this study also demonstrates that abiotic H2 generation by water reduction, widely studied in recent years in ultrabasic contexts, can also occur in sedimentary contexts where siderite is present. We show that, in the latter case, natural H2 concentration will be buffered by a condensed carbon phase associated with magnetite.

  7. Exploring the cell: Sodium (beta-alumina) cupric chloride - Aluminum chloride - Sodium chloride between 136 and 200 C

    NASA Technical Reports Server (NTRS)

    Miller, R. O.

    1975-01-01

    Experiments were done with a molten-salt catholyte (initially CuCl2 in AlCl3-NaCl) separated from molten Na by beta alumina. The open-circuit reduction potentials were 4.3 and 3 volts for Cu++ and Cu+, respectively. High polarization and nonrechargeability characterized the cell's operation. The cell's ohmic resistance during discharge was higher than what would be expected from only the ionic resistance of the beta-alumina.

  8. Static and Turn-on Switching Characteristics of 4H-Silicon Carbide SITs to 200 deg C

    NASA Technical Reports Server (NTRS)

    Niedra, Janis M.; Schwarze, Gene E.

    2005-01-01

    Test results are presented for normally-off 4H-SiC Static Induction Transistors (SITs) intended for power switching and are among the first normally-off such devices realized in SiC. At zero gate bias, the gate p-n junction depletion layers extend far enough into the conduction channel to cut off the channel. Application of forward gate bias narrows the depletion regions, opening up the channel to conduction by majority carriers. In the present devices, narrow vertical channels get pinched by depletion regions from opposite sides. Since the material is SiC, the devices are usable at temperatures above 150 C. Static curve and pulse mode switching observations were done at selected temperatures up to 200 C on a device with average static characteristics from a batch of similar devices. Gate and drain currents were limited to about 400 mA and 3.5 A, respectively. The drain voltage was limited to roughly 300 V, which is conservative for this 600 V rated device. At 23 C, 1 kW, or even more, could be pulse mode switched in 65 ns (10 to 90 percent) into a 100 load. But at 200 C, the switching capability is greatly reduced in large part by the excessive gate current required. Severe collapse of the saturated drain-to-source current was observed at 200 C. The relation of this property to channel mobility is reviewed.

  9. Productivity cycles of 200-300 years in the Antarctic Peninsula region: Understanding linkages among the sun, atmosphere, oceans, sea ice, and biota

    USGS Publications Warehouse

    Leventer, A.; Domack, E.W.; Ishman, S.E.; Brachfeld, S.; McClennen, C.E.; Manley, P.

    1996-01-01

    Compared to the rest of the world's oceans, high-resolution late Holocene paleoclimatic data from the Southern Ocean are still rare. We present a multiproxy record from a sediment core retrieved from a deep basin on the western side of the Antarctic Peninsula that reveals a dramatic perspective on paleoclimatic changes over the past 3700 yr. Analyses completed include measurement of magnetic susceptibility and granulometry, bed thickness, particle size, percent organic carbon, bulk density, and microscopic evaluation of diatom and benthic foraminiferal assemblages and abundances. Downcore variability of these parameters demonstrates the significance of both short-term cycles, which recur approximately every 200 yr, and longer term events (???2500 yr cycles) that are most likely related to global climatic fluctuations. In the upper 600 cm of the core, lower values of magnetic susceptibility (MS) are correlated with lower bulk density, the presence of thinly laminated units, specific diatom assemblages, and generally higher total organic carbon content. Below 600 cm, magnetic susceptibility is uniformly low, though variability in other parameters continues. The magnetic susceptibility signal is controlled primarily by dilution of ferromagnetic phases with biosiliceous material. This signal may be enhanced further by dissolution of magnetite in the magnetic susceptibility lows (high total organic carbon). The role of variable primary productivity and its relationship to paleoclimate is assessed through the diatom data. In particular, magnetic susceptibility lows are characterized by higher than normal abundances of Chaetoceros resting spores. Corethron criophilum and/or Rhizosolenia spp. also are found, as is a higher ratio of the most common species of Fragilariopsis versus species of Thalassiosira. These assemblages are indicative of periods of high primary productivity driven by the presence of a melt-water stabilized water column. The 200 yr cyclicity noted in

  10. 940nm QCW diode laser bars with 70% efficiency at 1 kW output power at 203K: analysis of remaining limits and path to higher efficiency and power at 200K and 300K

    NASA Astrophysics Data System (ADS)

    Frevert, C.; Bugge, F.; Knigge, S.; Ginolas, A.; Erbert, G.; Crump, P.

    2016-03-01

    Both high-energy-class laser facilities and commercial high-energy pulsed laser sources require reliable optical pumps with the highest pulse power and electro-optical efficiency. Although commercial quasi-continuous wave (QCW) diode laser bars reach output powers of 300…500 W further improvements are urgently sought to lower the cost per Watt, improve system performance and reduce overall system complexity. Diode laser bars operating at temperatures of around 200 K show significant advances in performance, and are particularly attractive in systems that use cryogenically cooled solid state lasers. We present the latest results on 940 nm, passively cooled, 4 mm long QCW diode bars which operate under pulse conditions of 1.2 ms, 10 Hz at an output power of 1 kW with efficiency of 70% at 203 K: a two-fold increase in power compared to 300 K, without compromising efficiency. We discuss how custom low-temperature design of the vertical layers can mitigate the limiting factors such as series resistance while sustaining high power levels. We then focus on the remaining obstacles to higher efficiency and power, and use a detailed study of multiple vertical structures to demonstrate that the properties of the active region are a major performance limit. Specifically, one key limit to series resistance is transport in the layers around the active region and the differential internal efficiency is closely correlated to the threshold current. Tailoring the barriers around the active region and reducing transparency current density thus promise bars with increased performance at temperatures of 200 K as well as 300 K.

  11. 75 FR 13046 - Airworthiness Directives; The Boeing Company Model 747-200C, -200F, -400, -400D, and -400F Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-18

    ... February 27, 2006, we issued AD 2006-05-09, amendment 39-14506 (71 FR 12122, March 9, 2006), for certain... resulted from fatigue tests and an analysis that identified areas of the fuselage lap joints where fatigue... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR...

  12. 76 FR 38074 - Airworthiness Directives; The Boeing Company Model 747-100, 747-100B, 747-200B, 747-200C, 747...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-29

    ... On October 1, 1990, we issued AD 90-21-17, amendment 39-6768 (55 FR 41510, October 12, 1990), for... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3... amendment 39-6768 (55 FR 41510, October 12, 1990) and adding the following new AD: The Boeing...

  13. 20 CFR 664.205 - How is the “deficient in basic literacy skills” criterion in § 664.200(c)(1) defined and documented?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... problems, reads, writes, or speaks English at or below the 8th grade level on a generally accepted... problems, read, write, or speak English at a level necessary to function on the job, in the...

  14. 20 CFR 664.205 - How is the “deficient in basic literacy skills” criterion in § 664.200(c)(1) defined and documented?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...) Computes or solves problems, reads, writes, or speaks English at or below the 8th grade level on a... to compute or solve problems, read, write, or speak English at a level necessary to function on...

  15. Plastic substrates for active matrix liquid crystal display incapable of withstanding processing temperature of over 200 C and method of fabrication

    DOEpatents

    Carey, P.G.; Smith, P.M.; Havens, J.H.; Jones, P.

    1999-01-05

    Bright-polarizer-free, active-matrix liquid crystal displays (AMLCDs) are formed on plastic substrates. The primary components of the display are a pixel circuit fabricated on one plastic substrate, an intervening liquid-crystal material, and a counter electrode on a second plastic substrate. The-pixel circuit contains one or more thin-film transistors (TFTs) and either a transparent or reflective pixel electrode manufactured at sufficiently low temperatures to avoid damage to the plastic substrate. Fabrication of the TFTs can be carried out at temperatures less than 100 C. The liquid crystal material is a commercially made nematic curvilinear aligned phase (NCAP) film. The counter electrode is comprised of a plastic substrate coated with a transparent conductor, such as indium-doped tin oxide (ITO). By coupling the active matrix with NCAP, a high-information content can be provided in a bright, fully plastic package. Applications include any low cost portable electronics containing flat displays where ruggedization of the display is desired. 12 figs.

  16. Participation of miR-200 in Pulmonary Fibrosis

    PubMed Central

    Yang, Shanzhong; Banerjee, Sami; de Freitas, Andressa; Sanders, Yan Y.; Ding, Qiang; Matalon, Sadis; Thannickal, Victor J.; Abraham, Edward; Liu, Gang

    2012-01-01

    Excessive extracellular matrix production by fibroblasts in response to tissue injury contributes to fibrotic diseases, such as idiopathic pulmonary fibrosis (IPF). Epithelial-mesenchymal transition, involving transition of alveolar epithelial cells (AECs) to pulmonary fibroblasts, appears to be an important contributory process to lung fibrosis. Although aberrant expression of microRNAs (miRs) is involved in a variety of pathophysiologic processes, the role of miRs in fibrotic lung diseases is less well understood. In the present study, we found that miR-200a, miR-200b, and miR-200c are significantly down-regulated in the lungs of mice with experimental lung fibrosis. Levels of miR-200a and miR-200c were reduced in the lungs of patients with IPF. miR-200 had greater expression in AECs than in lung fibroblasts, and AECs from mice with experimental pulmonary fibrosis had diminished expression of miR-200. We found that the miR-200 family members inhibit transforming growth factor-β1–induced epithelial-mesenchymal transition of AECs. miR-200 family members can reverse the fibrogenic activity of pulmonary fibroblasts from mice with experimental pulmonary fibrosis and from patients with IPF. Indeed, the introduction of miR-200c diminishes experimental pulmonary fibrosis in mice. Thus, the miR-200 family members participate importantly in fibrotic lung diseases and suggest that restoring miR-200 expression in the lungs may represent a novel therapeutic approach in treating pulmonary fibrotic diseases. PMID:22189082

  17. Topographical map series in the Map Room of Institute and Museum of Military History in Budapest, Hungary between 1919-1945: Smaller scale series (1:100,000, 1:200,000, 1:300,000, 1:500,000, 1:750,000) of the territory of Central Europe

    NASA Astrophysics Data System (ADS)

    Jankó, A.; Bánfi, R.

    2009-04-01

    The Royal Hungarian State Mapping Institute kept the smaller scales series of the third military survey of the Austro-Hungarian Monarchy, too, so the scales 1:200,000 and 1:750,000 maps. The results of the supervisions of larger scales were transferred onto these scales, 1:200,000 and 1:750,000 maps, for the territory of Central Europe. In 1943 a scale 1:500,000 aerial map was accomplished, too, for the territory of Pannonian basin. There are many other important series in the Map Room between 1919 and 1945, including the WWII German edition 1:300,000 scale map series of Central Europe and Russia to the Ural Mts.; and a series of scale 1:100,000 for the territory of Poland and Russia between 1939-1940.

  18. 29 CFR 99.200 - Audit requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 1 2014-07-01 2013-07-01 true Audit requirements. 99.200 Section 99.200 Labor Office of....200 Audit requirements. (a) Audit required. Non-Federal entities that expend $300,000 or more in a year in Federal awards (or $500,000 for fiscal years ending after December 31, 2003) shall have...

  19. 29 CFR 99.200 - Audit requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 1 2010-07-01 2010-07-01 true Audit requirements. 99.200 Section 99.200 Labor Office of....200 Audit requirements. (a) Audit required. Non-Federal entities that expend $300,000 or more in a year in Federal awards (or $500,000 for fiscal years ending after December 31, 2003) shall have...

  20. 29 CFR 99.200 - Audit requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 1 2013-07-01 2013-07-01 false Audit requirements. 99.200 Section 99.200 Labor Office of....200 Audit requirements. (a) Audit required. Non-Federal entities that expend $300,000 or more in a year in Federal awards (or $500,000 for fiscal years ending after December 31, 2003) shall have...

  1. 29 CFR 99.200 - Audit requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 1 2011-07-01 2011-07-01 false Audit requirements. 99.200 Section 99.200 Labor Office of....200 Audit requirements. (a) Audit required. Non-Federal entities that expend $300,000 or more in a year in Federal awards (or $500,000 for fiscal years ending after December 31, 2003) shall have...

  2. 29 CFR 99.200 - Audit requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 1 2012-07-01 2012-07-01 false Audit requirements. 99.200 Section 99.200 Labor Office of....200 Audit requirements. (a) Audit required. Non-Federal entities that expend $300,000 or more in a year in Federal awards (or $500,000 for fiscal years ending after December 31, 2003) shall have...

  3. 34 CFR 300.300 - Parental consent.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Parental consent. 300.300 Section 300.300 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES Evaluations,...

  4. 200 Deg C Demonstration Transformer Operates Efficiently at 50 kHz

    NASA Technical Reports Server (NTRS)

    Niedra, Janis M.; Schwarze, Gene E. (Technical Monitor)

    2003-01-01

    A compact, high temperature demonstration transformer was constructed, using a moly permalloy powder core and Teflon -insulated copper wire. At 50 kHz and 200 C, this 1:2 ratio transformer is capable of 98 percent efficiency when operating at a specific power of 6.1 kW/kg at 4 kW. This roughly 7 cm diameter transformer has a mass of 0.65 kg. Although Teflon is unstable above 200 C, about the same electrical performance was seen at 250 C. A plot of winding loss versus frequency illustrates the need to control these losses at high frequency.

  5. Hybrid A/D converter for 200 deg C operation

    NASA Technical Reports Server (NTRS)

    Sullivan, M. R.; Toth, J. B.

    1981-01-01

    A 12 bit A/D converter was designed and developed which will operate at 200 C with .05 linearity, 1/accuracy, 350 WSec conversion time, and only 455 mW power consumption. This product also necessitated the development of a unique three metal system in which aluminum wire bonding is done utilizing aluminum bonding pads, gold wire bonding to all gold areas, and employment of a nickel interface between gold and aluminum connections. This system totally eliminates the formation of a intermetallics at the bonding interface which can lead to bond failure. This product represents an advancement in electronics as it proved the operation of integrated circuits at high temperature, as well as providing information about both the electrical and mechanical reliability of hybrid circuits at 200 C.

  6. 50 CFR 300.200 - Purpose and scope.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... (“Moratorium Protection Act”) to identify and certify nations whose vessels are engaged in illegal, unreported... nations that are taking action to combat illegal, unreported, or unregulated fishing or reduce bycatch...

  7. 50 CFR 300.200 - Purpose and scope.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... language applies to vessels entitled to fly the flag of the nation in question. Where the Secretary of... prohibit the importation of certain fish and fish products from the identified nation or other...

  8. 50 CFR 300.200 - Purpose and scope.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... language applies to vessels entitled to fly the flag of the nation in question. Where the Secretary of... prohibit the importation of certain fish and fish products from the identified nation or other...

  9. 34 CFR 300.300 - Parental consent.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... DISABILITIES Evaluations, Eligibility Determinations, Individualized Education Programs, and Educational Placements Parental Consent § 300.300 Parental consent. (a) Parental consent for initial evaluation. (1)(i) The public agency proposing to conduct an initial evaluation to determine if a child qualifies as...

  10. 75 FR 78596 - Airworthiness Directives; Hawker Beechcraft Corporation Models B200, B200GT, B300, and B300C...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-16

    ... March 27, 2008, we issued AD 2008-07-10, Amendment 39-15451 (73 FR 18706, April 7, 2008), for certain... Executive Order 12866, (2) Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR... amends Sec. 39.13 by removing airworthiness directive (AD) 2008-07-10, Amendment 39-15451 (73 FR...

  11. 76 FR 24345 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-02

    ... December 28, 2010 (75 FR 81508). That NPRM proposed to require, for certain airplanes, replacing certain... Policies and Procedures (44 FR 11034, February 26, 1979), (3) Will not affect intrastate aviation in Alaska... fault interrupter (GFI) relays. For certain other airplanes, this AD requires installing new panels...

  12. 75 FR 81508 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ...: You may send comments by any of the following methods: Federal eRulemaking Portal: Go to http://www...'' (66 FR 23086, May 7, 2001). In addition to new airworthiness standards for transport airplanes and new... repairing (cleaning of applicable components with solvent) or replacing (replacing applicable...

  13. 75 FR 70102 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-17

    ... Management, P.O. Box 3707, MC 2H-65, Seattle, Washington 98124-2207; telephone 206- 544-5000, extension 1... airplanes. That NPRM was published in the Federal Register on April 8, 2010 (75 FR 17889). That NPRM... the NRPM to ensure a high level of safety for the Model 777 airplane fleet. Request to Include...

  14. 75 FR 80738 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-23

    ... NPRM was published in the Federal Register on August 7, 2008 (73 FR 45893). That original NPRM proposed... is already mandated by AD 2008- 11-13, Amendment 39-15536 (73 FR 30737, May 29, 2008), which is... 12866, 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR...

  15. 76 FR 13074 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-10

    ... Register on December 1, 2010 (75 FR 74668, January 18, 2011). That NPRM proposed to require repetitive... Policies and Procedures (44 FR 11034, February 26, 1979), (3) Will not affect intrastate aviation in Alaska... AD was prompted by a report indicating that a Karon-lined bushing with the liner broken into...

  16. 75 FR 74668 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-01

    ... 12866, 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... bushing with the liner broken into five pieces was found during a scheduled inspection of the HSTA... that a Karon-lined bushing with the liner broken into five pieces was found during a...

  17. 76 FR 2846 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-18

    ... 12866, (2) Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... airplanes, and wrapping fire-resistant silicone tape around the hydraulic tube for certain other airplanes... an adjacent hydraulic tube, creating a hole in the tube and spraying hydraulic fluid into the...

  18. 76 FR 63163 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, and -300ER Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-12

    ... (76 FR 2846). That NPRM proposed to require installing foreign object debris (FOD) rubber shields over... (76 FR 2846, January 18, 2011). The National Transportation Safety Board supports the NPRM. Request To... Include Information Notices United Airlines asked that we revise the NPRM (76 FR 2846, January 18,...

  19. 34 CFR 300.300 - Parental consent.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ....504, obtain informed consent, consistent with § 300.9, from the parent of the child before conducting... efforts to obtain the informed consent from the parent for an initial evaluation to determine whether the... with a disability must obtain informed consent from the parent of the child before the...

  20. 34 CFR 300.300 - Parental consent.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ....504, obtain informed consent, consistent with § 300.9, from the parent of the child before conducting... efforts to obtain the informed consent from the parent for an initial evaluation to determine whether the... with a disability must obtain informed consent from the parent of the child before the...

  1. 34 CFR 300.300 - Parental consent.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ....504, obtain informed consent, consistent with § 300.9, from the parent of the child before conducting... efforts to obtain the informed consent from the parent for an initial evaluation to determine whether the... with a disability must obtain informed consent from the parent of the child before the...

  2. miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes.

    PubMed

    Perdigão-Henriques, R; Petrocca, F; Altschuler, G; Thomas, M P; Le, M T N; Tan, S M; Hide, W; Lieberman, J

    2016-01-14

    The miR-200 family promotes the epithelial state by suppressing the Zeb1/Zeb2 epithelial gene transcriptional repressors. To identify other miR-200-regulated genes, we isolated mRNAs bound to transfected biotinylated miR-200c in mouse breast cancer cells. In all, 520 mRNAs were significantly enriched in miR-200c binding at least twofold. Putative miR-200-regulated genes included Zeb2, enriched 3.5-fold in the pull down. However, Zeb2 knockdown does not fully recapitulate miR-200c overexpression, suggesting that regulating other miR-200 targets contributes to miR-200's enhancement of epithelial gene expression. Candidate genes were highly enriched for miR-200c seed pairing in their 3'UTR and coding sequence and for genes that were downregulated by miR-200c overexpression. Epidermal growth factor receptor and downstream MAPK signaling pathways were the most enriched pathways. Genes whose products mediate transforming growth factor (TGF)-β signaling were also significantly overrepresented, and miR-200 counteracted the suppressive effects of TGF-β and bone morphogenic protein 2 (BMP-2) on epithelial gene expression. miR-200c regulated the 3'UTRs of 12 of 14 putative miR-200c-binding mRNAs tested. The extent of mRNA binding to miR-200c strongly correlated with gene suppression. Twelve targets of miR-200c (Crtap, Fhod1, Smad2, Map3k1, Tob1, Ywhag/14-3-3γ, Ywhab/14-3-3β, Smad5, Zfp36, Xbp1, Mapk12, Snail1) were experimentally validated by identifying their 3'UTR miR-200 recognition elements. Smad2 and Smad5 form a complex with Zeb2 and Ywhab/14-3-3β and Ywhag/14-3-3γ form a complex with Snail1. These complexes that repress transcription assemble on epithelial gene promoters. miR-200 overexpression induced RNA polymerase II localization and reduced Zeb2 and Snail1 binding to epithelial gene promoters. Expression of miR-200-resistant Smad5 modestly, but significantly, reduced epithelial gene induction by miR-200. miR-200 expression and Zeb2 knockdown are known to

  3. 300 AREA URANIUM CONTAMINATION

    SciTech Connect

    BORGHESE JV

    2009-07-02

    {sm_bullet} Uranium fuel production {sm_bullet} Test reactor and separations experiments {sm_bullet} Animal and radiobiology experiments conducted at the. 331 Laboratory Complex {sm_bullet} .Deactivation, decontamination, decommissioning,. and demolition of 300 Area facilities

  4. Brittle behavior of a dilute copper-beryllium alloy at 200 C in air. [Cu-0. 3Be-0. 5Co and Cu-0. 3Be-0. 5Co-0. 04Zr

    SciTech Connect

    Misra, R.D.K.; McMahon, C.J. Jr.; Guha, A. . Dept. of Materials Science and Engineering); Guha, A. )

    1994-12-01

    Intergranular cracking in a Cu-Be alloy tested in low-cycle fatigue at various partial pressures of oxygen was observed more than 20 years ago by Coffin. This phenomenon has also been observed in other copper alloys. Minute additions of zirconium were shown to improve the high temperature ductility of these alloys. This led to experiments to determine the effects of a zirconium addition to a cobalt-containing high strength, high conductivity Cu-Be alloy which showed intergranular cracking during hot-deformation processing. It is of interest to examine this phenomenon in the light of recent studies of dynamic embrittlement related to sulfur-induced intergranular cracking of low-alloy steels (stress-relief cracking) and to tin-induced intergranular cracking of Cu-Sn alloys. The dynamic embrittlement phenomenon involves the ingress of low-melting surface-adsorbed elements along grain boundaries under the influence of an applied tensile stress, in the manner of Coble creep. At any given temperature, the rate of ingress of low-melting surface-adsorbed elements along grain boundaries under the influence of an applied tensile stress, in the manner of Coble creep. At any given temperature, the rate of ingress, and thus the rate of cracking, varies with some power of the local stress. Because Cu-Be alloys can support very high stresses, one would anticipate that they could be susceptible to dynamic embrittlement in the presence of oxygen, a common embrittling element in metals. Accordingly, some preliminary experiments have been carried out on a dilute Cu-Be alloy with and without the presence of zirconium to see if its behavior is consistent with the dynamic embrittlement phenomenon.

  5. To designate the federal building currently known as Federal Office Building 8, located at 200 C Street Southwest in the District of Columbia, as the "Thomas P. O'Neill, Jr. Federal Building".

    THOMAS, 112th Congress

    Rep. Pelosi, Nancy [D-CA-8

    2012-11-27

    11/29/2012 Received in the Senate. (All Actions) Notes: For further action, see S.3687, which became Public Law 112-237 on 12/28/2012. Tracker: This bill has the status Passed HouseHere are the steps for Status of Legislation:

  6. Physics 300 Provincial Examination.

    ERIC Educational Resources Information Center

    Manitoba Dept. of Education and Training, Winnipeg.

    This document consists of the physics 300 provincial examination (English version), a separate "provincial summary report" on the results of giving the test, and a separate French language version of the examination. This physics examination contains a 53-item multiple choice section and an 12 item free response section. Subsections of the test…

  7. 34 CFR 300.801-300.802 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false 300.801-300.802 Section 300.801-300.802 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN...

  8. 300 Area Disturbance Report

    SciTech Connect

    LL Hale; MK Wright; NA Cadoret

    1999-01-07

    The objective of this study was to define areas of previous disturbance in the 300 Area of the U.S. Department of Energy (DOE) Hanford Site to eliminate these areas from the cultural resource review process, reduce cultural resource monitoring costs, and allow cultural resource specialists to focus on areas where subsurface disturbance is minimal or nonexistent. Research into available sources suggests that impacts from excavations have been significant wherever the following construction activities have occurred: building basements and pits, waste ponds, burial grounds, trenches, installation of subsurface pipelines, power poles, water hydrants, and well construction. Beyond the areas just mentioned, substrates in the' 300 Area consist of a complex, multidimen- sional mosaic composed of undisturbed stratigraphy, backfill, and disturbed sediments; Four Geographic Information System (GIS) maps were created to display known areas of disturbance in the 300 Area. These maps contain information gleaned from a variety of sources, but the primary sources include the Hanford GIS database system, engineer drawings, and historic maps. In addition to these maps, several assumptions can be made about areas of disturbance in the 300 Area as a result of this study: o o Buried pipelines are not always located where they are mapped. As a result, cultural resource monitors or specialists should not depend on maps depicting subsurface pipelines for accurate locations of previous disturbance. Temporary roads built in the early 1940s were placed on layers of sand and gravel 8 to 12 in. thick. Given this information, it is likely that substrates beneath these early roads are only minimally disturbed. Building foundations ranged from concrete slabs no more than 6 to 8 in. thick to deeply excavated pits and basements. Buildings constructed with slab foundations are more numerous than may be expected, and minimally disturbed substrates may be expected in these locations. Historic black

  9. English 200: Intermediate Composition

    ERIC Educational Resources Information Center

    Ritter, Kelly

    2005-01-01

    "English 200: Intermediate Composition" is a program elective for English majors and a writing-intensive elective for nonmajors at Southern Connecticut State University (SCSU), a comprehensive institution of 11,000 undergraduate and graduate (master's level) students. English 200 is described in the departmental course catalog as a course "in…

  10. 24 CFR 200.200 - What is the Appraiser Roster?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false What is the Appraiser Roster? 200.200 Section 200.200 Housing and Urban Development Regulations Relating to Housing and Urban... HOUSING AND URBAN DEVELOPMENT GENERAL INTRODUCTION TO FHA PROGRAMS Appraiser Roster § 200.200 What is...

  11. 25 CFR 200.1-200.10 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 1 2012-04-01 2011-04-01 true 200.1-200.10 Section 200.1-200.10 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS TERMS AND CONDITIONS: COAL LEASES §§ 200.1-200.10...

  12. 25 CFR 200.1-200.10 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 1 2013-04-01 2013-04-01 false 200.1-200.10 Section 200.1-200.10 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS TERMS AND CONDITIONS: COAL LEASES §§ 200.1-200.10...

  13. 25 CFR 200.1-200.10 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false 200.1-200.10 Section 200.1-200.10 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS TERMS AND CONDITIONS: COAL LEASES §§ 200.1-200.10...

  14. 25 CFR 200.1-200.10 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 1 2011-04-01 2011-04-01 false 200.1-200.10 Section 200.1-200.10 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS TERMS AND CONDITIONS: COAL LEASES §§ 200.1-200.10...

  15. 25 CFR 200.1-200.10 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 1 2014-04-01 2014-04-01 false 200.1-200.10 Section 200.1-200.10 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS TERMS AND CONDITIONS: COAL LEASES §§ 200.1-200.10...

  16. CYBER 200 Applications Seminar

    NASA Technical Reports Server (NTRS)

    Gary, J. P. (Compiler)

    1984-01-01

    Applications suited for the CYBER 200 digital computer are discussed. Various areas of application including meteorology, algorithms, fluid dynamics, monte carlo methods, petroleum, electronic circuit simulation, biochemistry, lattice gauge theory, economics and ray tracing are discussed.

  17. Tracker 300 Software

    SciTech Connect

    Wysor, R. Wes

    2006-01-12

    The Tracker300 software is downloaded to an off-the-shelf product called RCM3400/RCM3410 made by Rabbit Semiconductor. The software is a closed loop control which computes the sun's position and provides stability compensation. Using the RCM3400/RCM3410 module, the software stores and retrieves parameters from the onboard flash. The software also allows for communication with a host. It will allow the parameters to be downloaded or uploaded, it will show the status of the controller, it will provide real-time feedback, and it will send command acknowledgements. The software will capture the GPS response and ensure the internal clock is set correctly.

  18. Tracker 300 Software

    2006-01-12

    The Tracker300 software is downloaded to an off-the-shelf product called RCM3400/RCM3410 made by Rabbit Semiconductor. The software is a closed loop control which computes the sun's position and provides stability compensation. Using the RCM3400/RCM3410 module, the software stores and retrieves parameters from the onboard flash. The software also allows for communication with a host. It will allow the parameters to be downloaded or uploaded, it will show the status of the controller, it will providemore » real-time feedback, and it will send command acknowledgements. The software will capture the GPS response and ensure the internal clock is set correctly.« less

  19. Microeconomics of 300-mm process module control

    NASA Astrophysics Data System (ADS)

    Monahan, Kevin M.; Chatterjee, Arun K.; Falessi, Georges; Levy, Ady; Stoller, Meryl D.

    2001-08-01

    Simple microeconomic models that directly link metrology, yield, and profitability are rare or non-existent. In this work, we validate and apply such a model. Using a small number of input parameters, we explain current yield management practices in 200 mm factories. The model is then used to extrapolate requirements for 300 mm factories, including the impact of simultaneous technology transitions to 130nm lithography and integrated metrology. To support our conclusions, we use examples relevant to factory-wide photo module control.

  20. Status of EXO-200

    SciTech Connect

    Ackerman, Nicole; /SLAC

    2011-12-06

    EXO-200 is the first phase of the Enriched Xenon Observatory (EXO) experiment, which searches for neutrinoless double beta decay in {sup 136}Xe to measure the mass and probe the Majorana nature of the neutrino. EXO-200 consists of 200 kg of liquid Xe enriched to 80% in {sup 136}Xe in an ultra-low background TPC. Energy resolution is enhanced through the simultaneous collection of scintillation light using Large Area Avalanche Photodiodes (LAAPD's) and ionization charge. It is being installed at the WIPP site in New Mexico, which provides a 2000 meter water-equivalent overburden. EXO-200 will begin taking data in 2009, with the expected two-year sensitivity to the half-life for neutrinoless double beta decay of 6.4 x 10{sup 25} years. According to the most recent nuclear matrix element calculations, this corresponds to an effective Majorana neutrino mass of 0.13 to 0.19 eV. It will also measure the two neutrino mode for the first time in {sup 136}Xe.

  1. 34 CFR 200.92-200.99 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false 200.92-200.99 Section 200.92-200.99 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION TITLE I-IMPROVING THE ACADEMIC ACHIEVEMENT OF THE DISADVANTAGED Prevention...

  2. 34 CFR 200.101-200.102 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false 200.101-200.102 Section 200.101-200.102 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION TITLE I-IMPROVING THE ACADEMIC ACHIEVEMENT OF THE DISADVANTAGED General...

  3. 34 CFR 200.68-200.69 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false 200.68-200.69 Section 200.68-200.69 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION TITLE I-IMPROVING THE ACADEMIC ACHIEVEMENT OF THE DISADVANTAGED Improving...

  4. 34 CFR 200.104-200.109 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false 200.104-200.109 Section 200.104-200.109 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION TITLE I-IMPROVING THE ACADEMIC ACHIEVEMENT OF THE DISADVANTAGED General...

  5. 34 CFR 200.23-200.24 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false 200.23-200.24 Section 200.23-200.24 Education Regulations of the Offices of the Department of Education OFFICE OF ELEMENTARY AND SECONDARY EDUCATION, DEPARTMENT OF EDUCATION TITLE I-IMPROVING THE ACADEMIC ACHIEVEMENT OF THE DISADVANTAGED Improving...

  6. 75 FR 42589 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes, and A340-200, -300...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... specified products. That supplemental NPRM was published in the Federal Register on March 29, 2010 (75 FR... Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic impact, positive or... AD: Within 24 months after the effective date of this AD, perform simultaneous detailed...

  7. 75 FR 15353 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes, and A340-200, -300...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-29

    ... 13, 2009 (74 FR 1649). That earlier NPRM proposed to correct an unsafe condition for the products... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... this AD, perform simultaneous detailed visual inspections of the IPGB and of the DDS on all flap...

  8. 75 FR 12657 - Airworthiness Directives; The Boeing Company Model 777-200, -200LR, -300, -300ER, and 777F Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-17

    ... Policies and Procedures (44 FR 11034, February 26, 1979), and (3) Will not have a significant economic... ground, and from the discovery during flight simulations that the climb gradient is less than optimal for... flight director system, in addition to the two recent reports and the short compliance time involved...

  9. 75 FR 3125 - Airworthiness Directives; Airbus Model A330-200, A330-300, A340-200, and A340-300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-20

    ...We are adopting a new airworthiness directive (AD) for the products listed above. This AD results from mandatory continuing airworthiness information (MCAI) originated by an aviation authority of another country to identify and correct an unsafe condition on an aviation product. The MCAI describes the unsafe condition...

  10. 75 FR 54536 - Airworthiness Directives; Airbus Model A330-200 and -300 and A340-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-08

    ..., Amendment 39-14329 (70 FR 59263, October 12, 2005). That AD required actions intended to address an unsafe... Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic impact, positive or...), 40113, 44701. Sec. 39.13 2. The FAA amends Sec. 39.13 by removing Amendment 39-14329 (70 FR...

  11. 75 FR 4710 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes and A340-200, -300...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-29

    ... this proposed AD. Discussion On February 22, 2007, we issued AD 2007-05-08, Amendment 39-14969 (72 FR... rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will.... 39.13 by removing Amendment 39-14969 (72 FR 9658, March 5, 2007) and adding the following new...

  12. 75 FR 39814 - Airworthiness Directives; Airbus Model A330-200 and -300, and Model A340-200, -300, -500 and -600...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-13

    ... products. That NPRM was published in the Federal Register on December 29, 2009 (74 FR 68737). That NPRM... Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic impact, positive or... airworthiness directive (AD) for the products listed above. This AD results from mandatory...

  13. 75 FR 39790 - Airworthiness Directives; Airbus Model A330-200 and -300 Airplanes and Model A340-200, -300, -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-13

    ... by reference of certain publications listed in this AD as of August 17, 2010. On June 26, 2007 (72 FR... NPRM was published in the Federal Register on September 25, 2009 (74 FR 48872), and proposed to supersede AD 2007-18-04, Amendment 39- 15184 (72 FR 50042, August 30, 2007). That NPRM proposed to...

  14. 76 FR 6543 - Airworthiness Directives; Airbus Model A330-200 and -300 and A340-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-07

    ...We are superseding an existing airworthiness directive (AD) that applies to the products listed above. This AD results from mandatory continuing airworthiness information (MCAI) originated by an aviation authority of another country to identify and correct an unsafe condition on an aviation product. The MCAI describes the unsafe condition...

  15. 34 CFR 300.18 - Highly qualified special education teachers.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... profession under 34 CFR 200.56(c) which may include a single, high objective uniform State standard of... EDUCATION OF CHILDREN WITH DISABILITIES General Definitions Used in This Part § 300.18 Highly qualified... subjects, the term highly qualified has the meaning given the term in section 9101 of the ESEA and 34...

  16. 34 CFR 300.18 - Highly qualified special education teachers.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... profession under 34 CFR 200.56(c) which may include a single, high objective uniform State standard of... EDUCATION OF CHILDREN WITH DISABILITIES General Definitions Used in This Part § 300.18 Highly qualified... subjects, the term highly qualified has the meaning given the term in section 9101 of the ESEA and 34...

  17. 75 FR 82337 - Airworthiness Directives; The Boeing Company Model 777-200, -300, and -300ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-30

    ...; installing airplane information management system 2 (AIMS-2) software in the AIMS-2 hardware; and making... (AIMS-2) software in the AIMS-2 hardware, in accordance with the Accomplishment Instructions of Boeing... ] override/jettison and main jettison fuel pumps. This proposed AD would also require installing new...

  18. 76 FR 22305 - Airworthiness Directives; The Boeing Company Model 777-200, -300, and -300ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-21

    ... (AIMS-2) software in the AIMS-2 hardware; and making certain wiring changes. This AD was prompted by... (AIMS-2) software in the AIMS-2 hardware; and making certain wiring changes. Comments We gave the public... information management system 2 (AIMS-2) software in the AIMS-2 hardware, in accordance with...

  19. 76 FR 13534 - Airworthiness Directives; The Boeing Company Model 767-200, -300, -300F, and -400ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... proposed AD. Discussion On October 19, 2009, we issued AD 2009-22-13, amendment 39-16066 (74 FR 55755... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3... amendment 39-16066 (74 FR 55755, October 29, 2009) and adding the following new AD: The Boeing...

  20. 78 FR 52107 - Special Conditions: Boeing Model 777-200, -300, and -300ER Series Airplanes; Rechargeable Lithium...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-22

    ... April 11, 2000 (65 FR 19477-19478), as well as at http://DocketsInfo.dot.gov/ gov/. Docket: Background... Series Airplanes; Rechargeable Lithium Ion Batteries and Battery Systems AGENCY: Federal Aviation... lithium ion batteries and battery system that will be used on an International Communications Group...

  1. 76 FR 52899 - Airworthiness Directives; The Boeing Company Model 767-200, -300, and -300F Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-24

    ... Procedures (44 FR 11034, February 26, 1979), (3) Will not affect intrastate aviation in Alaska, and (4) Will... after receipt. FOR FURTHER INFORMATION CONTACT: Ana Martinez Hueto, Aerospace Engineer, Cabin Safety and... district office. Related Information (i) For more information about this AD, contact Ana Martinez...

  2. 78 FR 68986 - Special Conditions: Boeing Model 777-200, -300, and -300ER Series Airplanes; Aircraft Electronic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-18

    ... and connectivity capabilities of the airplane's onboard network computer systems, which may allow access to or by external computer systems and networks. This onboard network system will be composed of a... airplane's onboard network system. The applicable airworthiness regulations do not contain adequate...

  3. 75 FR 42592 - Airworthiness Directives; The Boeing Company Model 767-200, -300, and -300F Series Airplanes...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-22

    ... 13, 2005, we issued AD 2005-19-23, amendment 39-14288 (70 FR 55519, September 22, 2005). That AD... (65 Certain Boeing Model 767-54A0094 Repetitive inspections FR 18883, April 10, 2000). 767 series...-16-12, amendment 39-13768 (69 Certain Boeing Model 767-54-0069, 767-54- Modification of the FR...

  4. 75 FR 34663 - Airworthiness Directives; The Boeing Company Model 777-200, -300, and -300ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-18

    ... series airplanes. That original NPRM was published in the Federal Register on January 29, 2007 (72 FR... Policies and Procedures (44 FR 11034, February 26, 1979), and 3. Will not have a significant economic... series airplanes. The original NPRM would have required installing Teflon sleeving under the clamps...

  5. 75 FR 5677 - Airworthiness Directives; The Boeing Company Model 767-200, -300, and -300F Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-04

    ... publication listed in the AD as of March 5, 2001 (66 FR 8085, January 29, 2001). The Director of the Federal... as of October 17, 2000 (65 FR 58641, October 2, 2000). We must receive any comments on this AD by.... SUPPLEMENTARY INFORMATION: Discussion On January 22, 2003, we issued AD 2003-03-02, amendment 39-13026 (68...

  6. 76 FR 10288 - Airworthiness Directives; The Boeing Company Model 767-200, -300, -300F, and -400ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... proposed AD. Discussion On September 26, 2005, we issued AD 2004-19-06 R1, amendment 39- 14313 (70 FR 58000... a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26...-06 R1, Amendment 39-14313 (70 FR 58000, October 5, 2005), and adding the following new AD: The...

  7. 75 FR 78588 - Airworthiness Directives; The Boeing Company Model 777-200, -300, and -300ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-16

    ... supplemental NPRM was published in the Federal Register on June 18, 2010 (75 FR 34663). The original NPRM (72 FR 3956, January 29, 2007) proposed to require installing Teflon sleeving under ] the clamps of... Policies and Procedures (44 FR 11034, February 26, 1979), and (3) Will not have a significant...

  8. 78 FR 70849 - Special Conditions: Boeing Model 777-200, -300, and -300ER Series Airplanes; Aircraft Electronic...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-27

    ... Series Airplanes; Aircraft Electronic System Security Protection from Unauthorized Internal Access... regulatory adequacy under Sec. 611 of Public Law 92-574, the ``Noise Control Act of 1972.'' The FAA issues... between systems, including: 1. Flight-safety related control and navigation systems, 2. Operator...

  9. 75 FR 3656 - Airworthiness Directives; The Boeing Company Model 767-200, -300, and -300F Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-22

    ... oxygen system to melt or burn, resulting in oxygen system leakage and smoke or fire. Relevant Service... Policies and Procedures (44 FR 11034, February 26, 1979), and 3. Will not have a significant economic... supernumerary oxygen systems to melt or burn, resulting in oxygen system leakage and smoke or fire....

  10. 75 FR 47180 - Airworthiness Directives; The Boeing Company Model 767-200, -300, and -300F Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-05

    ... oxygen systems to melt or burn, resulting in oxygen system leakage and smoke or fire. DATES: This AD is... NPRM was published in the Federal Register on January 22, 2010 (75 FR 3656). That NPRM proposed to... given the potential consequence of an oxygen-fed fire in the vicinity of the flightcrew station. We...

  11. Dynamics and plasticity of the epithelial to mesenchymal transition induced by miR-200 family inhibition

    PubMed Central

    Haraguchi, Takeshi; Kondo, Masayuki; Uchikawa, Ryo; Kobayashi, Kazuyoshi; Hiramatsu, Hiroaki; Kobayashi, Kyousuke; Chit, Ung Weng; Shimizu, Takanobu; Iba, Hideo

    2016-01-01

    Whereas miR-200 family is known to be involved in the epithelial-to-mesenchymal transition (EMT), a crucial biological process observed in normal and pathological contexts, it has been largely unclear how far the functional levels of these tiny RNAs alone can propagate the molecular events to accomplish this process within several days. By developing a potent inhibitor of miR-200 family members (TuD-141/200c), the expression of which is strictly regulatable by the Tet (tetracycline)-On system, we found using a human colorectal cell line, HCT116, that several direct gene target mRNAs (Zeb1/Zeb2, ESRP1, FN1and FHOD1) of miR-200 family were elevated with distinct kinetics. Prompt induction of the transcriptional suppressors, Zeb1/Zeb2 in turn reduced the expression levels of miR-200c/-141 locus, EpCAM, ESRP1 and E-Cad. The loss of ESRP1 subsequently switched the splicing isoforms of CD44 and p120 catenin mRNAs to mesenchymal type. Importantly, within 9 days after the release from the inhibition of miR-200 family, all of the expression changes in the 14 genes observed in this study returned to their original levels in the epithelial cells. This suggests that the inherent epithelial plasticity is supported by a weak retention of key regulatory gene expression in either the epithelial or mesenchymal states through epigenetic regulation. PMID:26887353

  12. 76 FR 13541 - Airworthiness Directives; The Boeing Company Model 757-200, -200CB, and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ...-11266 (64 FR 45436, August 20, 1999), which was based on Boeing Service Bulletin 757-25-0182, Revision 1... Procedures (44 FR 11034, February 26, 1979), (3) Will not affect intrastate aviation in Alaska, and (4) Will... the bearings and lockbase retainer in the door latch assembly, relocating and adjusting of the...

  13. 76 FR 1351 - Airworthiness Directives; The Boeing Company Model 757-200, -200CB, and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-10

    ... the lap joint at stringer 4L (left) of the chem-milled step. The airplane had accumulated 24,631 total... interior surface along the chem-milled step edges above the stringer 4L or 4R (right) lap splices of the... Executive Order 12866, (2) Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44...

  14. MicroRNA-200b Impacts Breast Cancer Cell Migration and Invasion by Regulating Ezrin-Radixin-Moesin

    PubMed Central

    Hong, Hong; Yu, Haizhong; Yuan, Jianfen; Guo, Chunyan; Cao, Hongyan; Li, Weibing; Xiao, Chunhong

    2016-01-01

    Background Ezrin-radixin-moesin (ERM) plays an important role in multiple links of tumors. It also involved in breast cancer invasion and metastasis, and might be a potential biomarker of breast cancer. Another study suggested that ERM expression was regulated directly by miR-200c, and had a critical role in miR-200c suppressing cell migration. This study aimed to investigate the effect of miR-200b on ERM expression in a breast cancer cell line and its influence on invasion and metastasis ability in vitro. Material/Methods Breast cancer cell lines MCF-7 and MDA-MB-231 with different metastatic potentials were selected as a model. MiR-200b overexpression or inhibition was achieved by Lipofectamine™ 2000-mediated miRNA transfection. RT-PCR was used to test miR-200b level, while Western blot was selected to detect ERM protein expression. Wound healing assay and Transwell assay were performed to determine cell migration and invasion ability. Results RT-PCR revealed that miR-200b level in MDA-MB-231 was obviously lower than that in MCF-7, while Western blot analysis showed that ERM expression was significantly higher. MiR-200b inhibition by transfection in MCF-7 markedly decreased miR-200b level, elevated ERM expression, and enhanced cell migration and invasion. MiR-200b overexpression in MDA-MB-231 obviously increased miR-200b level, reduced ERM expression, and weakened cell migration and invasion. Conclusions MiR-200b participates in breast cancer cell migration and invasion through regulating ERM in MCF-7 and MDA-MB-231. PMID:27276064

  15. 300 Junior Novel Anticipation Guides

    ERIC Educational Resources Information Center

    Polette, Nancy

    2006-01-01

    In this book, the author has created reproducible anticipation guides to 300 popular junior novels (grades 4-8) ranging from classics like Ben and Me and Cricket in Times Square to the current award winner, Kira, Kira. The book consists of 300 reproducible sets of open ended values questions to pique the interest of readers. Librarians and…

  16. MicroRNA-200 Family Modulation in Distinct Breast Cancer Phenotypes

    PubMed Central

    Sarrió, David; Romero-Pérez, Laura; López-García, María Ángeles; Vieites, Begoña; Biscuola, Michele; Ramiro-Fuentes, Susana; Isacke, Clare M.; Palacios, José

    2012-01-01

    The epithelial to mesenchymal transition (EMT) contributes to tumor invasion and metastasis in a variety of cancer types. In human breast cancer, gene expression studies have determined that basal-B/claudin-low and metaplastic cancers exhibit EMT-related characteristics, but the molecular mechanisms underlying this observation are unknown. As the family of miR-200 microRNAs has been shown to regulate EMT in normal tissues and cancer, here we evaluated whether the expression of the miR-200 family (miR-200f) and their epigenetic state correlate with EMT features in human breast carcinomas. We analyzed by qRT-PCR the expression of miR-200f members and various EMT-transcriptional inducers in a series of 70 breast cancers comprising an array of phenotypic subtypes: estrogen receptor positive (ER+), HER2 positive (HER2+), and triple negative (TN), including a subset of metaplastic breast carcinomas (MBCs) with sarcomatous (homologous or heterologous) differentiation. No MBCs with squamous differentiation were included. The DNA methylation status of miR-200f loci in tumor samples were inspected using Sequenom MassArray® MALDI-TOF platform. We also used two non-tumorigenic breast basal cell lines that spontaneously undergo EMT to study the modulation of miR-200f expression during EMT in vitro. We demonstrate that miR-200f is strongly decreased in MBCs compared with other cancer types. TN and HER2+ breast cancers also exhibited lower miR-200f expression than ER+ tumors. Significantly, the decreased miR-200f expression found in MBCs is accompanied by an increase in the expression levels of EMT-transcriptional inducers, and hypermethylation of the miR-200c-141 locus. Similar to tumor samples, we demonstrated that downregulation of miR-200f and hypermethylation of the miR-200c-141 locus, together with upregulation of EMT-transcriptional inducers also occur in an in vitro cellular model of spontaneous EMT. Thus, the expression and methylation status of miR-200f could be used

  17. Operational status of TAMA300

    NASA Astrophysics Data System (ADS)

    Takahashi, Ryutaro; TAMA Collaboration

    2003-09-01

    The project of a laser interferometer gravitational wave detector TAMA300 is in progress. The 300 m Fabry Perot Michelson interferometer was successfully locked with power recycling in December 2001. The sensitivity was improved owing to further frequency stabilization as well as additional power gain. The seventh data taking run was held as the first international coincidence run with Laser Interferometer Gravitational Wave Observatory (LIGO) and GEO600. Duty cycle of TAMA300 was 76.7%. Five detectors were under the coincidence observation for 9.8 h during the 34 h run.

  18. Rabbit CD200R binds host CD200 but not CD200-like proteins from poxviruses

    PubMed Central

    Akkaya, Munir; Kwong, Lai-Shan; Akkaya, Erdem; Hatherley, Deborah; Barclay, A. Neil

    2016-01-01

    CD200 is a widely distributed membrane protein that gives inhibitory signals through its receptor (CD200R) on myeloid cells. CD200 has been acquired by herpesviruses where it has been shown to interact with host CD200R and downmodulate the immune system. It has been hypothesized that poxviruses have acquired CD200; but the potential orthologues show less similarity to their hosts. Myxoma virus M141 protein is a potential CD200 orthologue with a potent immune modulatory function in rabbits. Here, we characterized the rabbit CD200, CD200R and tested the CD200-like sequences for binding CD200R. No binding could be detected using soluble recombinant proteins, full length protein expressed on cells or myxoma virus infected cells. Finally, using knockdown models, we showed that the inhibitory effect of M141 on RAW 264.7 cells upon myxoma virus infection is not due to CD200R. We conclude that the rabbit poxvirus CD200-like proteins cause immunomodulation without utilizing CD200R. PMID:26590792

  19. Rabbit CD200R binds host CD200 but not CD200-like proteins from poxviruses.

    PubMed

    Akkaya, Munir; Kwong, Lai-Shan; Akkaya, Erdem; Hatherley, Deborah; Barclay, A Neil

    2016-01-15

    CD200 is a widely distributed membrane protein that gives inhibitory signals through its receptor (CD200R) on myeloid cells. CD200 has been acquired by herpesviruses where it has been shown to interact with host CD200R and downmodulate the immune system. It has been hypothesized that poxviruses have acquired CD200; but the potential orthologues show less similarity to their hosts. Myxoma virus M141 protein is a potential CD200 orthologue with a potent immune modulatory function in rabbits. Here, we characterized the rabbit CD200, CD200R and tested the CD200-like sequences for binding CD200R. No binding could be detected using soluble recombinant proteins, full length protein expressed on cells or myxoma virus infected cells. Finally, using knockdown models, we showed that the inhibitory effect of M141 on RAW 264.7 cells upon myxoma virus infection is not due to CD200R. We conclude that the rabbit poxvirus CD200-like proteins cause immunomodulation without utilizing CD200R. PMID:26590792

  20. 34 CFR 300.709 - Public participation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Public participation. 300.709 Section 300.709 Education... Interior § 300.709 Public participation. In fulfilling the requirements of § 300.708 the Secretary of the Interior must provide for public participation consistent with § 300.165. (Authority: 20 U.S.C. 1411(h))...

  1. Ice core profiles of saturated fatty acids (C12:0-C30:0) and oleic acid (C18:1) from southern Alaska since 1734 AD: A link to climate change in the Northern Hemisphere

    NASA Astrophysics Data System (ADS)

    Pokhrel, Ambarish; Kawamura, Kimitaka; Seki, Osamu; Matoba, Sumio; Shiraiwa, Takayuki

    2015-01-01

    An ice core drilled at Aurora Peak in southeast Alaska was analyzed for homologous series of straight chain fatty acids (C12:0-C30:0) including unsaturated fatty acid (oleic acid) using gas chromatography (GC/FID) and GC/mass spectrometry (GC/MS). Molecular distributions of fatty acids are characterized by even carbon number predominance with a peak at palmitic acid (C16:0, av. 20.3 ± SD. 29.8 ng/g-ice) followed by oleic acid (C18:1, 19.6 ± 38.6 ng/g-ice) and myristic acid (C14:0, 15.3 ± 21.9 ng/g-ice). The historical trends of short-chain fatty acids, together with correlation analysis with inorganic ions and organic tracers suggest that short-chain fatty acids (except for C12:0 and C15:0) were mainly derived from sea surface micro layers through bubble bursting mechanism and transported over the glacier through the atmosphere. This atmospheric transport process is suggested to be linked with Kamchatka ice core δD record from Northeast Asia and Greenland Temperature Anomaly (GTA). In contrast, long-chain fatty acids (C20:0-C30:0) are originated from terrestrial higher plants, soil organic matter and dusts, which are also linked with GTA. Hence, this study suggests that Alaskan fatty acids are strongly influenced by Pacific Decadal Oscillation/North Pacific Gyre Oscillation and/or extra tropical North Pacific surface climate and Arctic oscillation. We also found that decadal scale variability of C18:1/C18:0 ratios in the Aurora Peak ice core correlate with the Kamchatka ice core δD, which reflects climate oscillations in the North Pacific. This study suggests that photochemical aging of organic aerosols could be controlled by climate periodicity.

  2. 50 CFR 300.39 - Exceptions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Pacific Tuna Fisheries § 300.39 Exceptions. (a) The prohibitions of § 300.38 and the licensing requirements of § 300.32 do not apply to fishing for albacore tuna by vessels using the trolling method or...

  3. 50 CFR 300.39 - Exceptions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Pacific Tuna Fisheries § 300.39 Exceptions. (a) The prohibitions of § 300.38 and the licensing requirements of § 300.32 do not apply to fishing for albacore tuna by vessels using the trolling method or...

  4. Preliminary Evaluation of PS300: A New Self-Lubricating High Temperature Composite Coating for Use to 800 C

    NASA Technical Reports Server (NTRS)

    Dellacorte, C.; Edmonds, B. J.

    1995-01-01

    This paper introduces PS300, a plasma sprayed, self-lubricating composite coating for use in sliding contacts at temperatures to 800 C. PS300 is a metal bonded chrome oxide coating with silver and BaF2/CaF2 eutectic solid lubricant additives. PS300 is similar to PS200, a chromium carbide based coating, which is currently being investigated for a variety of tribological applications. In pin-on-disk testing up to 650 C, PS300 exhibited comparable friction and wear properties to PS200. The PS300 matrix, which is predominantly chromium oxide rather than chromium carbide, does not require diamond grinding and polishes readily with silicon carbide abrasives greatly reducing manufacturing costs compared to PS200. It is anticipated that PS300 has potential for sliding bearing and seal applications in both aerospace and general industry.

  5. The HWP-300 wind turbine

    NASA Astrophysics Data System (ADS)

    Jamieson, P.; McLeish, D.

    1983-12-01

    A 300 kW, 22 m diameter wind turbine has been designed and erected (July 1983) on the site of the North of Scotland Hydro-Electric Board at Burgar Hill in Orkney. The machine and its design concepts are described.

  6. 76 FR 53303 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-26

    ...-210-AD; Amendment 39-16772; AD 2011-17-08] RIN 2120-AA64 Airworthiness Directives; Airbus Model A330... reference of a certain other publication listed in this AD as of June 7, 2006 (71 FR 25919, May 3, 2006... (76 FR 15867), and proposed to supersede AD 2006-09-07, Amendment 39-14577 (71 FR 25919, May 3,...

  7. 76 FR 18038 - Airworthiness Directives; Airbus Model A340-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-04-01

    ... lock. * * * * * Deployment of one TR door in flight, particularly during the take-off or go around, could result in heavy buffet at low speed, or could significantly reduce take off performance [and..., particularly during the take-off or go around, could result in heavy buffet at low speed, or...

  8. Quarterly environmental radiological survey summary third quarter 1997 100, 200, 300, and 600 Areas

    SciTech Connect

    McKinney, S.M.

    1997-10-28

    Routine radiological surveys are part of near-facility environmental monitoring which monitors and helps direct the reduction of the radiological areas at the Hanford Site. The routine radiological surveys are performed by the Southern Area Remediation Support Group and the Site Support Services Radiological Control Group as directed by Environmental Monitoring and Investigations. The surveys included in this program consist of inactive waste sites; outdoor radiological control areas; tank farm perimeters and associated diversion boxes, lift stations, and vent stations; perimeters of active or uncovered waste sites such as burial grounds, retention basins, ponds, process trenches, and ditches; underground pipelines; and road and rail surfaces. This report provides a summary of the radiological surveys performed during the Third Quarter of 1997. The status of corrective actions required from current and past reports are also discussed. A waste site survey schedule, WHC-SP-0098-8, was developed by Environmental Monitoring and Investigations and reviewed by the Southern Area Remediation Support Group and the Site Support Services Radiological Control Group. Environmental Monitoring and Investigations reviews the radiological survey reports and files a copy for historical purposes and reference. Radiological conditions are tracked and trends noted. All sites are surveyed at least once each year. The survey frequencies for particular sites are based on site history, radiological conditions, and general maintenance. Special surveys may be conducted at irregular frequencies if conditions warrant (e.g., growth of deep-rooted vegetation is noted at a waste site). Radiological surveys are conducted to detect surface contamination and document changes in vegetation growth, biological intrusion, erosion, and general site maintenance conditions. Survey data are compared with standards identified in WHC-CM-7-5, Environmental Compliance, as well as previous surveys to recognize possible trends, assess environmental impacts, and help determine where corrective actions are needed. Landlords of the sites found out of compliance may be issued a Radiological Problem Report (RPR) from the appropriate radiological Control Group. open Surveillance Compliance Inspection Reports (SCIRS) and compliance Assessment Reports (CARS) are listed in this report. The surveys scheduled for this program consist of inactive waste sites; outdoor radiological areas; tank farm perimeters and associated diversion boxes, lift stations, and vent stations; perimeters of active or uncovered waste sites such as burial grounds, retention basins, ponds, process trenches, and ditches; underground pipelines; and road and rail surfaces.

  9. 76 FR 6578 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-07

    ... Federal Register on August 4, 2010 (75 FR 46861). The original NPRM (75 FR 4710, January 29, 2010... Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic.... 106(g), 40113, 44701. Sec. 39.13 2. The FAA amends Sec. 39.13 by removing Amendment 39-14969 (72...

  10. 76 FR 37255 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-27

    ... reference of a certain other publication listed in this AD as of April 9, 2007 (72 FR 9658, March 5, 2007... on February 7, 2011 (76 FR 6578), and proposed to supersede AD 2007-05-08, Amendment 39-14969 (72 FR..., 2. Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034,...

  11. 75 FR 46861 - Airworthiness Directives; Airbus Model A330-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-04

    ... 29, 2010 (75 FR 4710). That earlier NPRM proposed to supersede AD 2007-05-08, Amendment 39-14969 (72 FR 96580, March 5, 2007), to require actions intended to address the unsafe condition for the..., -500, and -600 series airplanes. We have issued AD 2010-01-07, Amendment 39-16165 (75 FR 1538,...

  12. 75 FR 38945 - Airworthiness Directives; The Boeing Company Model 777-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-07

    ... Procedures (44 FR 11034, February 26, 1979), and 3. Will not have a significant economic impact, positive or... software in the cabin management system, and loading new software into the mass memory card. This proposed... follows: At the cabin system control panel (CSCP), remove the installed mass memory card (MMC)...

  13. Quarterly Environmental Radiological Survey Summary Third Quarter 1998, 100, 200, 300, and 600 Areas

    SciTech Connect

    MCKINNEY, S.M.

    1998-10-27

    This report provides a summary of the radiological surveys performed in support of near-facility environmental monitoring at the Hanford Site. The Third Quarter 1998 survey results and the status of actions required are summarized: (1) All of the eighty-five environmental radiological surveys scheduled during July, August and September were performed as planned. Fifty-one of the surveys were conducted at Project Hanford Management Contractors (PHMC) sites and thirty-four at Environmental Restoration Contractor (ERC) sites. Contamination above background levels was found at seventeen of the PHMC waste sites and two of the ERC waste sites. Contamination levels as high >1,000,000 disintegrations per minute (dpm) per 100 cm{sup 2} were reported. Of these contaminated surveys nine were in Underground Radioactive Material (URM) areas, three were in unposted areas and seven were in contamination areas. The contamination found within four of the URM and three of the CA areas was immediately cleaned up and no further action was required. The remaining five URM and two unposted sites were posted and along with the five CA sites will require remediation. Radiological Problem Reports (RPR's) were issued and the sites were turned over to the landlord for further action as required. (2) During the second quarter of 1998, 1.2 hectares (3.0 acres) were stabilized and radiologically down posted from Contamination Area (CA)/Soil Contamination (SC) to URM. (3) Four hectares (10 acres) located south and west of B-Plant were posted as a radiological buffer area as a result of a contamination spread. This off-normal occurrence is currently being investigated. (4) Four Surveillance Compliance Inspection Reports (SCIRs) remained open and had not been resolved. Tank Farms Operations has responsibility for the unresolved SCIRs.

  14. Quarterly environmental radiological survey summary: Second Quarter 1995 100, 200, 300, and 600 Areas

    SciTech Connect

    McKinney, S.M.

    1995-07-27

    This report provides a summary of the radiological surveys performed in support of the operational environmental monitoring program at the Hanford Site. The Second Quarter 1995 survey results and the status of actions required from current and past reports are summarized.

  15. 76 FR 12617 - Airworthiness Directives; The Boeing Company Model 777-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-08

    ... system control panel, changing the wiring, installing a new electrical power control panel, and... to ensure that the flightcrew is able to turn off electrical power to the IFE system and other non... inability to turn off electrical power to the IFE system and other non-essential electrical systems...

  16. 76 FR 24351 - Airworthiness Directives; Airbus Model A340-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-02

    ...We are adopting a new airworthiness directive (AD) for the products listed above. This AD results from mandatory continuing airworthiness information (MCAI) originated by an aviation authority of another country to identify and correct an unsafe condition on an aviation product. The MCAI describes the unsafe condition...

  17. 76 FR 62661 - Airworthiness Directives; The Boeing Company Model 767-200 and -300 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    ... Procedures (44 FR 11034, February 26, 1979), (3) Will not affect intrastate aviation in Alaska, and (4) Will.... Send your comments to an address listed under the ADDRESSES section. Include ``Docket No. FAA-2011-1063... following new airworthiness directive (AD): The Boeing Company: Docket No. FAA-2011-1063;...

  18. Hanford 200 Areas Development Plan

    SciTech Connect

    Rinne, C.A.; Daly, K.S.

    1993-08-01

    The purpose of the Hanford 200 Areas Development Plan (Development Plan) is to guide the physical development of the 200 Areas (which refers to the 200 East Area, 200 West Area, and 200 Area Corridor, located between the 200 East and 200 West Areas) in accordance with US Department of Energy (DOE) Order 4320.lB (DOE 1991a) by performing the following: Establishing a land-use plan and setting land-use categories that meet the needs of existing and proposed activities. Coordinating existing, 5-year, and long-range development plans and guiding growth in accordance with those plans. Establishing development guidelines to encourage cost-effective development and minimize conflicts between adjacent activities. Identifying site development issues that need further analysis. Integrating program plans with development plans to ensure a logical progression of development. Coordinate DOE plans with other agencies [(i.e., Washington State Department of Ecology (Ecology) and US Environmental Protection Agency (EPA)]. Being a support document to the Hanford Site Development Plan (DOE-RL 1990a) (parent document) and providing technical site information relative to the 200 Areas.

  19. 34 CFR 200.56 - Definition of “highly qualified teacher.”

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... requirements for a “highly qualified special education teacher” in 34 CFR 300.18. (Approved by the Office of... 34 Education 1 2011-07-01 2011-07-01 false Definition of âhighly qualified teacher.â 200.56 Section 200.56 Education Regulations of the Offices of the Department of Education OFFICE OF...

  20. Catch tank inhibitor addition 200-East and 200-West Areas

    SciTech Connect

    Palit, A.N.

    1996-06-21

    Reported is the study of 11 catch tanks in the 200-East Area and the 7 catch tanks in the 200-West Area listed as active. The location, capacity, material of construction, annual total accumulation, annual rain intrusion, waste transfer rate, and access for chemical injection in these tanks are documented. The present and future utilization and isolation plans for the catch tanks are established.

  1. Nuclear criticality safety: 300 Area

    SciTech Connect

    Not Available

    1991-07-31

    This Standard applies to the receipt, processing, storage, and shipment of fissionable material in the 300 Area and in any other facility under the control of the Reactor Materials Project Management Team (PMT). The objective is to establish practices and process conditions for the storage and handling of fissionable material that prevent the accidental assembly of a critical mass and that comply with DOE Orders as well as accepted industry practice.

  2. 50 CFR 300.117 - Prohibitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Prohibitions. 300.117 Section 300.117 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Antarctic Marine Living Resources § 300.117 Prohibitions. In addition to the prohibitions in § 300.4, it is unlawful for any person to: (a) Reduce...

  3. 34 CFR 300.117 - Nonacademic settings.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Nonacademic settings. 300.117 Section 300.117 Education... DISABILITIES State Eligibility Least Restrictive Environment (lre) § 300.117 Nonacademic settings. In providing... meals, recess periods, and the services and activities set forth in § 300.107, each public agency...

  4. 11 CFR 300.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... connection with a Federal election as that term is used in 11 CFR 300.11, 300.37, 300.50, and 300.51 includes... a public communication (see 11 CFR 100.26). (c) Directly or indirectly establish, finance, maintain... its allocated share of proceeds jointly raised pursuant to 11 CFR 102.17, and otherwise...

  5. 11 CFR 300.2 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... connection with a Federal election as that term is used in 11 CFR 300.11, 300.37, 300.50, and 300.51 includes... a public communication (see 11 CFR 100.26). (c) Directly or indirectly establish, finance, maintain... its allocated share of proceeds jointly raised pursuant to 11 CFR 102.17, and otherwise...

  6. 34 CFR 300.327 - Educational placements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Educational placements. 300.327 Section 300.327... Educational Placements Development of Iep § 300.327 Educational placements. Consistent with § 300.501(c), each... makes decisions on the educational placement of their child. (Authority: 20 U.S.C. 1414(e))...

  7. 34 CFR 300.613 - Access rights.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Access rights. 300.613 Section 300.613 Education... § 300.613 Access rights. (a) Each participating agency must permit parents to inspect and review any... pursuant to § 300.510, and in no case more than 45 days after the request has been made. (b) The right...

  8. 34 CFR 300.512 - Hearing rights.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Hearing rights. 300.512 Section 300.512 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.512 Hearing rights. (a....534, or an appeal conducted pursuant to § 300.514, has the right to— (1) Be accompanied and advised...

  9. 50 CFR 300.104 - Scientific research.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Scientific research. 300.104 Section 300.104 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Antarctic Marine Living Resources § 300.104 Scientific research. (a) The management measures issued pursuant to the procedures at § 300.111...

  10. 34 CFR 300.623 - Safeguards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... instruction regarding the State's policies and procedures under § 300.123 and 34 CFR part 99. (d) Each... 34 Education 2 2010-07-01 2010-07-01 false Safeguards. 300.623 Section 300.623 Education... § 300.623 Safeguards. (a) Each participating agency must protect the confidentiality of...

  11. 34 CFR 300.623 - Safeguards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... instruction regarding the State's policies and procedures under § 300.123 and 34 CFR part 99. (d) Each... 34 Education 2 2011-07-01 2010-07-01 true Safeguards. 300.623 Section 300.623 Education... § 300.623 Safeguards. (a) Each participating agency must protect the confidentiality of...

  12. 34 CFR 300.121 - Procedural safeguards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Procedural safeguards. 300.121 Section 300.121... safeguards. (a) General. The State must have procedural safeguards in effect to ensure that each public agency in the State meets the requirements of §§ 300.500 through 300.536. (b) Procedural...

  13. 34 CFR 300.516 - Civil action.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Civil action. 300.516 Section 300.516 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.516 Civil action. (a... aggrieved by the findings and decision under § 300.514(b), has the right to bring a civil action...

  14. 34 CFR 300.516 - Civil action.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Civil action. 300.516 Section 300.516 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.516 Civil action. (a... aggrieved by the findings and decision under § 300.514(b), has the right to bring a civil action...

  15. 34 CFR 300.516 - Civil action.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Civil action. 300.516 Section 300.516 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.516 Civil action. (a... aggrieved by the findings and decision under § 300.514(b), has the right to bring a civil action...

  16. 34 CFR 300.516 - Civil action.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Civil action. 300.516 Section 300.516 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.516 Civil action. (a... aggrieved by the findings and decision under § 300.514(b), has the right to bring a civil action...

  17. 50 CFR 300.125 - Vessel identification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Vessel identification. 300.125 Section 300... REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.125 Vessel identification. (a) Official number. A vessel with a permit issued pursuant to § 300.123, when in treaty waters,...

  18. 50 CFR 300.125 - Vessel identification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Vessel identification. 300.125 Section 300... REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.125 Vessel identification. (a) Official number. A vessel with a permit issued pursuant to § 300.123, when in treaty waters,...

  19. 34 CFR 300.516 - Civil action.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Civil action. 300.516 Section 300.516 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.516 Civil action. (a... aggrieved by the findings and decision under § 300.514(b), has the right to bring a civil action...

  20. Neutral winds above 200 km at high latitudes.

    NASA Technical Reports Server (NTRS)

    Meriwether, J. W.; Heppner, J. P.; Stolarik, J. D.; Wescott, E. M.

    1973-01-01

    Electrically neutral, luminous clouds are a by-product of chemical releases conducted to create barium ion clouds for the measurement of electric fields. Wind measurements provided by the motions of these clouds are particularly valuable in that the motions can be directly compared with convective ion drift motions to test the importance of ion drag forces. Motion from multiple releases between 200 and 300 km from 15 rockets launched from four high-latitude locations is analyzed in this paper. The observations in the evening and midnight hours at magnetic latitudes above 65 deg strongly suggest that in these regions ion drag is the dominant force in driving neutral winds between 200 and 300 km. In the morning sector, it is evident that neutral wind observations cannot be directly interpreted in terms of ion drag; other factors must be considered.

  1. Anti-p200 pemphigoid.

    PubMed

    Goletz, Stephanie; Hashimoto, Takashi; Zillikens, Detlef; Schmidt, Enno

    2014-07-01

    Anti-p200 pemphigoid is a rare subepidermal blistering skin disease. Patients' autoantibodies label the dermal side of 1 mol/L NaCl-split human skin by indirect immunofluorescence microscopy and recognize a 200-kd protein by immunoblotting of human dermal extract. Clinically, anti-p200 pemphigoid is characterized by tense blisters and vesicles, erosions, and urticarial plaques, closely resembling bullous pemphigoid and the inflammatory variant of epidermolysis bullosa acquisita. Recently, 90% of anti-p200 pemphigoid sera were shown to recognize laminin γ1. The C-terminus of laminin γ1 was identified as an immunodominant region and in its recombinant form was used by immunoblotting and enzyme-linked immunosorbent assay for the serologic diagnosis of this disease. Subsequent ex vivo and in vivo studies were, however, unable to show pathogenic activity of antilaminin γ1 antibodies. Both patients' sera and sera depleted from antilaminin γ1 antibodies induced subepidermal splitting in an ex vivo model of autoantibody-mediated leukocyte-dependent neutrophil activation. Antilaminin γ1 antibodies appear to be useful biomarkers that will further facilitate the diagnosis of anti-p200 pemphigoid. The true identity of the pathogenetically relevant autoantigen of this disease, which may either be a yet unknown isoform of laminin γ1 or even another 200-kd protein of the dermoepidermal junction, still needs to be elucidated. PMID:24767733

  2. The microRNA-200 family: small molecules with novel roles in cancer development, progression and therapy

    PubMed Central

    Humphries, Brock; Yang, Chengfeng

    2015-01-01

    MicroRNAs (miRNAs) are a large family of small non-coding RNAs that negatively regulate protein-coding gene expression post-transcriptionally via base pairing between the 5′ seed region of a miRNA and the 3′ untranslated region (3′UTR) of a messenger RNA (mRNA). Recent evidence has supported the critical role that miRNAs play in many diseases including cancer. The miR-200 family consisting of 5 members (miR-200a, -200b, -200c, -141, -429) is an emerging miRNA family that has been shown to play crucial roles in cancer initiation and metastasis, and potentially be important for the diagnosis and treatment of cancer. While miR-200s were found to be critically involved in the metastatic colonization to the lungs in mouse mammary xenograft tumor models, a large number of studies demonstrated their strong suppressive effects on cell transformation, cancer cell proliferation, migration, invasion, tumor growth and metastasis. This review aims to discuss research findings about the role of the miR-200 family in cancer initiation, each step of cancer metastatic cascade, cancer diagnosis and treatment. A comprehensive summary of currently validated miR-200 targets is also presented. It is concluded that miR-200 family may serve as novel targets for the therapy of multiple types of cancer. PMID:25762624

  3. P 300 EVENT RELATED POTENTIAL IN DEPRESSION

    PubMed Central

    Singh, R.; Shukla, R.; Dalal, P.K.; Sinha, P.K.; Trivedi, J.K.

    2000-01-01

    P300 component of the event related potential (ERP) provides one neurophysiological index of cognitive dysfunction in depression. Forty subjects fulfilling DSM-III criteria for depression were compared to 40 age and sex matched normal controls. The P300 was recorded using the auditory odd-ball paradigm. Depressives had a significantly prolonged P300 latency and reduced P300 amplitude as compared to the controls. The P300 latency showed a significant positive correlation with age of the patient and severity of depression while P300 amplitude showed a significant negative correlation with age. The clinical subcategory of depression, duration of illness and sex did not show any relationship with P300 abnormality. Twelve out of 40 depressives (30%) had an abnormal P300. The mean Hamilton Rating Scale for Depression (HRSD) score was significantly high in those with an abnormal P300. PMID:21407978

  4. Cure of metastatic growth of EMT6 tumor cells in mice following manipulation of CD200:CD200R signaling.

    PubMed

    Gorczynski, Reginald M; Chen, Zhiqi; Khatri, Ismat; Podnos, Anna; Yu, Kai

    2013-11-01

    In previous studies, we observed that regulation of expression of CD200, both on cells of a transplantable breast cancer, EMT6, and of the host, as well as of the receptor, CD200R in host mice, regulated local tumor growth and metastasis in immunocompetent animals. This in turn led to an improved ability to document immunity to EMT6 in CD200R1KO mice. In the current study, we have explored the ability to cure BALB/c CD200KO or CD200R1KO mice of tumors ≤1 cm(3) in size by surgical resection of localized tumor, followed by immunization with irradiated EMT6 cells along with CpG as adjuvant. While control animals treated in this fashion developed significant pulmonary and liver metastases within 30 days of surgery, significant protection was seen in both CD200KO or CD200R1KO mice, with no macroscopic lung/liver metastases observed in CD200R1KO mice on sacrifice at day 300. Following surgical resection and immunization, draining lymph nodes from control mice contained tumor cells cloned at limiting dilution in vitro even before pulmonary and hepatic metastasis was seen. In contrast, within the limits of detection of the assay used (sensitivity ~1 in 10(7) cells), no tumor cells were detected at limiting dilution in similarly treated CD200R1KO mice, and significant reductions were seen in CD200KO mice. Infusion of anti-CD4, but less so anti-CD8, mAb into surgically treated and immunized CD200R1KO mice attenuated protection from both macroscopic (liver/lung) and microscopic (assayed by limiting dilution of DLN) metastasis. Adoptive transfer of lymphocytes from treated CD200R1KO mice to surgically treated control mice also attenuated metastatic growth of tumor, which was abolished by pretreatment of transferred cells with anti-CD4 mAb. Our data suggest that CD200:CD200R attenuates a potentially tumor-protective CD4 host response to breast cancer. PMID:24166280

  5. 50 CFR 300.31 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... South Pacific Tuna Fisheries § 300.31 Definitions. In addition to the terms defined in § 300.2, in the... (Torres Strait Protected Zone) Act, 1984. National Seas Act (Cap 361). Tuna Resources Management Act...

  6. 50 CFR 300.31 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... South Pacific Tuna Fisheries § 300.31 Definitions. In addition to the terms defined in § 300.2, in the... (Torres Strait Protected Zone) Act, 1984. National Seas Act (Cap 361). Tuna Resources Management Act...

  7. 50 CFR 300.31 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... South Pacific Tuna Fisheries § 300.31 Definitions. In addition to the terms defined in § 300.2, in the... (Torres Strait Protected Zone) Act, 1984. National Seas Act (Cap 361). Tuna Resources Management Act...

  8. 50 CFR 300.31 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... South Pacific Tuna Fisheries § 300.31 Definitions. In addition to the terms defined in § 300.2, in the... (Torres Strait Protected Zone) Act, 1984. National Seas Act (Cap 361). Tuna Resources Management Act...

  9. 50 CFR 300.31 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... South Pacific Tuna Fisheries § 300.31 Definitions. In addition to the terms defined in § 300.2, in the... (Torres Strait Protected Zone) Act, 1984. National Seas Act (Cap 361). Tuna Resources Management Act...

  10. Neutral Wind Observations below 200 km altitudes

    NASA Astrophysics Data System (ADS)

    Watanabe, S.; Abe, T.; Habu, H.; Kakinami, Y.; Larsen, M. F.; Pfaff, R. F., Jr.; Yamamoto, M.

    2015-12-01

    Neutral Wind Observations below 200 km altitudesS. Watanabe1, T. Abe2, H. Habu2, Y. Kakinami3, M. Larsen4, R. Pfaff5, M. Yamamoto6, M-Y. Yamamoto31Hokkaido University/Hokkaido Information University, 2JAXA/ISAS, 3Kochi University of Technology, 4Clemson University, 5NASA/Goddard Space Flight Center, 6Kyoto University, Neutral wind in the thermosphere is one of the key parameters to understand the ionosphere-thermosphere coupling process. JAXA/ISAS successfully launched sounding rockets from Uchinoura Space Center (USC) on September 2, 2007, January 12, 2012, and July 20, 2013, and NASA launched sounding rockets from Kwajalein on May 7, 2013 and from Wallops on July 4, 2013. The rockets installed Lithium and/or TMA canisters as well as instruments for plasma and electric and magnetic fields. The atomic Lithium gases were released at altitudes between 150 km and 300 km in the evening on September 2, 2007, at altitude of ~100 km in the morning on January 12, 2012, at altitude of ~120km in the midnight on July 20, 2013, at altitude between 150 km and 300 km in the evening on May 7, 2013 and at altitude of ~150 km in the noon on July 4, 2013. The Lithium atoms were scattering sunlight by resonance scattering with wavelength of 670nm. However, the Lithium atoms scattered moon light on July 20, 2013. The moon light scattering is the first time to use for thermospheric wind measurement in the midnight. The Lithium clouds/trails and TMA trails showed clearly the neutral wind shears and atmospheric waves at ~150 km altitude in the lower thermosphere for all local time.

  11. OM300 Direction Drilling Module

    DOE Data Explorer

    MacGugan, Doug

    2013-08-22

    OM300 – Geothermal Direction Drilling Navigation Tool: Design and produce a prototype directional drilling navigation tool capable of high temperature operation in geothermal drilling Accuracies of 0.1° Inclination and Tool Face, 0.5° Azimuth Environmental Ruggedness typical of existing oil/gas drilling Multiple Selectable Sensor Ranges High accuracy for navigation, low bandwidth High G-range & bandwidth for Stick-Slip and Chirp detection Selectable serial data communications Reduce cost of drilling in high temperature Geothermal reservoirs Innovative aspects of project Honeywell MEMS* Vibrating Beam Accelerometers (VBA) APS Flux-gate Magnetometers Honeywell Silicon-On-Insulator (SOI) High-temperature electronics Rugged High-temperature capable package and assembly process

  12. 11 CFR 300.30 - Accounts.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., 11 CFR 102.5(a)(4), 11 CFR 106.7(d)(1)(i), 11 CFR 300.33 and 11 CFR 300.34. (2) Levin account. The funds deposited into this account must comply with 11 CFR 300.31. Such funds may be used for the categories of activities described at 11 CFR 300.32(b). (3) Federal account. Federal accounts may be used...

  13. 11 CFR 300.30 - Accounts.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., 11 CFR 102.5(a)(4), 11 CFR 106.7(d)(1)(i), 11 CFR 300.33 and 11 CFR 300.34. (2) Levin account. The funds deposited into this account must comply with 11 CFR 300.31. Such funds may be used for the categories of activities described at 11 CFR 300.32(b). (3) Federal account. Federal accounts may be used...

  14. 24 CFR 300.3 - Description.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Description. 300.3 Section 300.3 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) GOVERNMENT NATIONAL MORTGAGE ASSOCIATION, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT GENERAL § 300.3 Description....

  15. 24 CFR 300.9 - Office.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Office. 300.9 Section 300.9 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) GOVERNMENT NATIONAL MORTGAGE ASSOCIATION, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT GENERAL § 300.9 Office....

  16. 24 CFR 300.15 - Exceptions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Exceptions. 300.15 Section 300.15 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) GOVERNMENT NATIONAL MORTGAGE ASSOCIATION, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT GENERAL § 300.15 Exceptions....

  17. 24 CFR 300.15 - Exceptions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Exceptions. 300.15 Section 300.15 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) GOVERNMENT NATIONAL MORTGAGE ASSOCIATION, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT GENERAL § 300.15 Exceptions....

  18. 24 CFR 300.3 - Description.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Description. 300.3 Section 300.3 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) GOVERNMENT NATIONAL MORTGAGE ASSOCIATION, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT GENERAL § 300.3 Description....

  19. 24 CFR 300.9 - Office.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Office. 300.9 Section 300.9 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) GOVERNMENT NATIONAL MORTGAGE ASSOCIATION, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT GENERAL § 300.9 Office....

  20. 24 CFR 300.9 - Office.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Office. 300.9 Section 300.9 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) GOVERNMENT NATIONAL MORTGAGE ASSOCIATION, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT GENERAL § 300.9 Office....

  1. 24 CFR 300.3 - Description.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Description. 300.3 Section 300.3 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) GOVERNMENT NATIONAL MORTGAGE ASSOCIATION, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT GENERAL § 300.3 Description....

  2. 24 CFR 300.15 - Exceptions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Exceptions. 300.15 Section 300.15 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) GOVERNMENT NATIONAL MORTGAGE ASSOCIATION, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT GENERAL § 300.15 Exceptions....

  3. 11 CFR 300.12 - Transition rules.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... effect between November 6 and December 31, 2002: 11 CFR 106.5(a),(b), (c), (f) and (g). ... 11 Federal Elections 1 2011-01-01 2011-01-01 false Transition rules. 300.12 Section 300.12 Federal... FUNDS National Party Committees § 300.12 Transition rules. (a) Permissible uses of excess...

  4. 34 CFR 300.606 - Public attention.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Public attention. 300.606 Section 300.606 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND..., and Enforcement § 300.606 Public attention. Whenever a State receives notice that the Secretary...

  5. 34 CFR 300.606 - Public attention.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Public attention. 300.606 Section 300.606 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND..., and Enforcement § 300.606 Public attention. Whenever a State receives notice that the Secretary...

  6. 34 CFR 300.509 - Model forms.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Model forms. 300.509 Section 300.509 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.509 Model forms. (a) Each SEA must develop model forms to assist parents and public agencies in filing a due...

  7. 34 CFR 300.509 - Model forms.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Model forms. 300.509 Section 300.509 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.509 Model forms. (a) Each SEA must develop model forms to assist parents and public agencies in filing a due...

  8. 34 CFR 300.509 - Model forms.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Model forms. 300.509 Section 300.509 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.509 Model forms. (a) Each SEA must develop model forms to assist parents and public agencies in filing a due...

  9. 34 CFR 300.509 - Model forms.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Model forms. 300.509 Section 300.509 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.509 Model forms. (a) Each SEA must develop model forms to assist parents and public agencies in filing a due...

  10. 34 CFR 300.809 - Limitations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Limitations. 300.809 Section 300.809 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND... DISABILITIES Preschool Grants for Children with Disabilities § 300.809 Limitations. (a) Notwithstanding §...

  11. 16 CFR 300.16 - Ornamentation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 16 Commercial Practices 1 2014-01-01 2014-01-01 false Ornamentation. 300.16 Section 300.16 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER THE WOOL PRODUCTS LABELING ACT OF 1939 Labeling § 300.16 Ornamentation. (a) Where the...

  12. 16 CFR 300.16 - Ornamentation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 16 Commercial Practices 1 2012-01-01 2012-01-01 false Ornamentation. 300.16 Section 300.16 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER THE WOOL PRODUCTS LABELING ACT OF 1939 Labeling § 300.16 Ornamentation. (a) Where the...

  13. 16 CFR 300.16 - Ornamentation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Ornamentation. 300.16 Section 300.16 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER THE WOOL PRODUCTS LABELING ACT OF 1939 Labeling § 300.16 Ornamentation. (a) Where the...

  14. 16 CFR 300.16 - Ornamentation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 16 Commercial Practices 1 2013-01-01 2013-01-01 false Ornamentation. 300.16 Section 300.16 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER THE WOOL PRODUCTS LABELING ACT OF 1939 Labeling § 300.16 Ornamentation. (a) Where the wool product contains fiber ornamentation...

  15. 16 CFR 300.16 - Ornamentation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 16 Commercial Practices 1 2010-01-01 2010-01-01 false Ornamentation. 300.16 Section 300.16 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER THE WOOL PRODUCTS LABELING ACT OF 1939 Labeling § 300.16 Ornamentation. (a) Where the...

  16. 34 CFR 300.505 - Electronic mail.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Electronic mail. 300.505 Section 300.505 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.505 Electronic mail. A....508 by an electronic mail communication, if the public agency makes that option available....

  17. 34 CFR 300.505 - Electronic mail.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Electronic mail. 300.505 Section 300.505 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.505 Electronic mail. A....508 by an electronic mail communication, if the public agency makes that option available....

  18. 34 CFR 300.505 - Electronic mail.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Electronic mail. 300.505 Section 300.505 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.505 Electronic mail. A....508 by an electronic mail communication, if the public agency makes that option available....

  19. 46 CFR 148.300 - Radioactive materials.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... radioactive materials defined in 49 CFR 173.403 as Low Specific Activity Material, LSA-1, or Surface... 46 Shipping 5 2014-10-01 2014-10-01 false Radioactive materials. 148.300 Section 148.300 Shipping... MATERIALS THAT REQUIRE SPECIAL HANDLING Special Requirements for Certain Materials § 148.300...

  20. 46 CFR 148.300 - Radioactive materials.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... radioactive materials defined in 49 CFR 173.403 as Low Specific Activity Material, LSA-1, or Surface... 46 Shipping 5 2011-10-01 2011-10-01 false Radioactive materials. 148.300 Section 148.300 Shipping... MATERIALS THAT REQUIRE SPECIAL HANDLING Special Requirements for Certain Materials § 148.300...

  1. 49 CFR 179.300-20 - Reports.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 3 2013-10-01 2013-10-01 false Reports. 179.300-20 Section 179.300-20...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-20 Reports. (a) Before a tank is placed in..., Mechanical Division, Association of American Railroads, a report in approved form certifying that the...

  2. 49 CFR 179.300-20 - Reports.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 3 2011-10-01 2011-10-01 false Reports. 179.300-20 Section 179.300-20...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-20 Reports. (a) Before a tank is placed in..., Mechanical Division, Association of American Railroads, a report in approved form certifying that the...

  3. 49 CFR 179.300-20 - Reports.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 3 2012-10-01 2012-10-01 false Reports. 179.300-20 Section 179.300-20...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-20 Reports. (a) Before a tank is placed in..., Mechanical Division, Association of American Railroads, a report in approved form certifying that the...

  4. 34 CFR 300.14 - Equipment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Equipment. 300.14 Section 300.14 Education Regulations... DISABILITIES General Definitions Used in This Part § 300.14 Equipment. Equipment means— (a) Machinery, utilities, and built-in equipment, and any necessary enclosures or structures to house the...

  5. 34 CFR 300.505 - Electronic mail.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Electronic mail. 300.505 Section 300.505 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.505 Electronic mail. A....508 by an electronic mail communication, if the public agency makes that option available....

  6. 34 CFR 300.505 - Electronic mail.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Electronic mail. 300.505 Section 300.505 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.505 Electronic mail. A....508 by an electronic mail communication, if the public agency makes that option available....

  7. 34 CFR 300.105 - Assistive technology.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Assistive technology. 300.105 Section 300.105 Education... DISABILITIES State Eligibility Other Fape Requirements § 300.105 Assistive technology. (a) Each public agency must ensure that assistive technology devices or assistive technology services, or both, as those...

  8. 40 CFR 82.300 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Purpose. 82.300 Section 82.300 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) PROTECTION OF STRATOSPHERIC OZONE Ban on Refrigeration and Air-Conditioning Appliances Containing HCFCs § 82.300 Purpose....

  9. 21 CFR 312.300 - General.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 5 2012-04-01 2012-04-01 false General. 312.300 Section 312.300 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS FOR HUMAN USE INVESTIGATIONAL NEW DRUG APPLICATION Expanded Access to Investigational Drugs for Treatment Use § 312.300...

  10. 21 CFR 312.300 - General.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 5 2014-04-01 2014-04-01 false General. 312.300 Section 312.300 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS FOR HUMAN USE INVESTIGATIONAL NEW DRUG APPLICATION Expanded Access to Investigational Drugs for Treatment Use § 312.300...

  11. 21 CFR 312.300 - General.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 5 2011-04-01 2011-04-01 false General. 312.300 Section 312.300 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS FOR HUMAN USE INVESTIGATIONAL NEW DRUG APPLICATION Expanded Access to Investigational Drugs for Treatment Use § 312.300...

  12. 21 CFR 312.300 - General.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 5 2010-04-01 2010-04-01 false General. 312.300 Section 312.300 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS FOR HUMAN USE INVESTIGATIONAL NEW DRUG APPLICATION Expanded Access to Investigational Drugs for Treatment Use § 312.300...

  13. 5 CFR 300.301 - Authority.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 1 2011-01-01 2011-01-01 false Authority. 300.301 Section 300.301 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Details of Employees § 300.301 Authority. (a) In accordance with 5 U.S.C. 3341, an agency may detail an employee in...

  14. 5 CFR 300.301 - Authority.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Authority. 300.301 Section 300.301 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Details of Employees § 300.301 Authority. (a) In accordance with 5 U.S.C. 3341, an agency may detail an employee in...

  15. 46 CFR 181.300 - Fire pumps.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Fire pumps. 181.300 Section 181.300 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Fire Main System § 181.300 Fire pumps. (a) A self priming, power driven fire pump must...

  16. 46 CFR 118.300 - Fire pumps.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Fire pumps. 118.300 Section 118.300 Shipping COAST GUARD... OVERNIGHT ACCOMMODATIONS FOR MORE THAN 49 PASSENGERS FIRE PROTECTION EQUIPMENT Fire Main System § 118.300 Fire pumps. (a) A self priming, power driven fire pump must be installed on each vessel. (b) On...

  17. 42 CFR 425.300 - Compliance plan.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 3 2012-10-01 2012-10-01 false Compliance plan. 425.300 Section 425.300 Public... Protections § 425.300 Compliance plan. (a) The ACO must have a compliance plan that includes at least the...) An ACO's compliance plan must be in compliance with and be updated periodically to reflect changes...

  18. 42 CFR 425.300 - Compliance plan.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 3 2013-10-01 2013-10-01 false Compliance plan. 425.300 Section 425.300 Public... Protections § 425.300 Compliance plan. (a) The ACO must have a compliance plan that includes at least the...) An ACO's compliance plan must be in compliance with and be updated periodically to reflect changes...

  19. 34 CFR 300.37 - Services plan.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Services plan. 300.37 Section 300.37 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND... DISABILITIES General Definitions Used in This Part § 300.37 Services plan. Services plan means a...

  20. 21 CFR 73.300 - Carrot oil.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false Carrot oil. 73.300 Section 73.300 Food and Drugs... ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.300 Carrot oil. (a) Identity. (1) The color additive carrot oil is the liquid or the solid portion of the mixture or the mixture itself obtained by the...

  1. 21 CFR 73.300 - Carrot oil.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 1 2012-04-01 2012-04-01 false Carrot oil. 73.300 Section 73.300 Food and Drugs... ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.300 Carrot oil. (a) Identity. (1) The color additive carrot oil is the liquid or the solid portion of the mixture or the mixture itself obtained by the...

  2. 21 CFR 73.300 - Carrot oil.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 1 2014-04-01 2014-04-01 false Carrot oil. 73.300 Section 73.300 Food and Drugs... ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.300 Carrot oil. (a) Identity. (1) The color additive carrot oil is the liquid or the solid portion of the mixture or the mixture itself obtained by the...

  3. 21 CFR 73.300 - Carrot oil.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 1 2013-04-01 2013-04-01 false Carrot oil. 73.300 Section 73.300 Food and Drugs... ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.300 Carrot oil. (a) Identity. (1) The color additive carrot oil is the liquid or the solid portion of the mixture or the mixture itself obtained by the...

  4. 21 CFR 73.300 - Carrot oil.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 1 2011-04-01 2011-04-01 false Carrot oil. 73.300 Section 73.300 Food and Drugs... ADDITIVES EXEMPT FROM CERTIFICATION Foods § 73.300 Carrot oil. (a) Identity. (1) The color additive carrot oil is the liquid or the solid portion of the mixture or the mixture itself obtained by the...

  5. 5 CFR 300.603 - Coverage.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 1 2012-01-01 2012-01-01 false Coverage. 300.603 Section 300.603 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Time-In-Grade Restrictions § 300.603 Coverage. (a) Coverage. This subpart applies to advancement to a...

  6. 5 CFR 300.402 - Coverage.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 1 2011-01-01 2011-01-01 false Coverage. 300.402 Section 300.402 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Use of Commercial Recruiting Firms and Nonprofit Employment Services § 300.402 Coverage. This part applies...

  7. 5 CFR 300.402 - Coverage.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Coverage. 300.402 Section 300.402 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Use of Commercial Recruiting Firms and Nonprofit Employment Services § 300.402 Coverage. This part applies...

  8. 5 CFR 300.702 - Coverage.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Coverage. 300.702 Section 300.702 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Statutory Bar to Appointment of Persons Who Fail To Register Under Selective Service Law § 300.702...

  9. 5 CFR 300.603 - Coverage.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Coverage. 300.603 Section 300.603 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Time-In-Grade Restrictions § 300.603 Coverage. (a) Coverage. This subpart applies to advancement to a...

  10. 5 CFR 300.502 - Coverage.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Coverage. 300.502 Section 300.502 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Use of Private Sector Temporaries § 300.502 Coverage. (a) These regulations apply to the competitive service...

  11. 5 CFR 300.603 - Coverage.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 1 2014-01-01 2014-01-01 false Coverage. 300.603 Section 300.603 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Time-In-Grade Restrictions § 300.603 Coverage. (a) Coverage. This subpart applies to advancement to a...

  12. 5 CFR 300.702 - Coverage.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 1 2011-01-01 2011-01-01 false Coverage. 300.702 Section 300.702 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Statutory Bar to Appointment of Persons Who Fail To Register Under Selective Service Law § 300.702...

  13. 5 CFR 300.502 - Coverage.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 1 2012-01-01 2012-01-01 false Coverage. 300.502 Section 300.502 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Use of Private Sector Temporaries § 300.502 Coverage. (a) These regulations apply to the competitive service...

  14. 5 CFR 300.502 - Coverage.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 1 2014-01-01 2014-01-01 false Coverage. 300.502 Section 300.502 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Use of Private Sector Temporaries § 300.502 Coverage. (a) These regulations apply to the competitive service...

  15. 5 CFR 300.502 - Coverage.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 1 2011-01-01 2011-01-01 false Coverage. 300.502 Section 300.502 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Use of Private Sector Temporaries § 300.502 Coverage. (a) These regulations apply to the competitive service...

  16. 5 CFR 300.702 - Coverage.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 1 2014-01-01 2014-01-01 false Coverage. 300.702 Section 300.702 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Statutory Bar to Appointment of Persons Who Fail To Register Under Selective Service Law § 300.702...

  17. 5 CFR 300.603 - Coverage.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 1 2013-01-01 2013-01-01 false Coverage. 300.603 Section 300.603 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Time-In-Grade Restrictions § 300.603 Coverage. (a) Coverage. This subpart applies to advancement to a...

  18. 5 CFR 300.502 - Coverage.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 1 2013-01-01 2013-01-01 false Coverage. 300.502 Section 300.502 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Use of Private Sector Temporaries § 300.502 Coverage. (a) These regulations apply to the competitive service...

  19. 5 CFR 300.402 - Coverage.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 1 2014-01-01 2014-01-01 false Coverage. 300.402 Section 300.402 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Use of Commercial Recruiting Firms and Nonprofit Employment Services § 300.402 Coverage. This part applies...

  20. 5 CFR 300.702 - Coverage.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 1 2012-01-01 2012-01-01 false Coverage. 300.702 Section 300.702 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Statutory Bar to Appointment of Persons Who Fail To Register Under Selective Service Law § 300.702...

  1. 5 CFR 300.702 - Coverage.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 1 2013-01-01 2013-01-01 false Coverage. 300.702 Section 300.702 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Statutory Bar to Appointment of Persons Who Fail To Register Under Selective Service Law § 300.702...

  2. 5 CFR 300.402 - Coverage.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 1 2013-01-01 2013-01-01 false Coverage. 300.402 Section 300.402 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Use of Commercial Recruiting Firms and Nonprofit Employment Services § 300.402 Coverage. This part applies...

  3. 5 CFR 300.603 - Coverage.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 1 2011-01-01 2011-01-01 false Coverage. 300.603 Section 300.603 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Time-In-Grade Restrictions § 300.603 Coverage. (a) Coverage. This subpart applies to advancement to a...

  4. 5 CFR 300.402 - Coverage.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 1 2012-01-01 2012-01-01 false Coverage. 300.402 Section 300.402 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS EMPLOYMENT (GENERAL) Use of Commercial Recruiting Firms and Nonprofit Employment Services § 300.402 Coverage. This part applies...

  5. 34 CFR 300.702 - Technical assistance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... to support technical assistance activities authorized under section 616(i) of the Act. (b) Maximum... 34 Education 2 2011-07-01 2010-07-01 true Technical assistance. 300.702 Section 300.702 Education..., and Use of Funds § 300.702 Technical assistance. (a) In general. The Secretary may reserve not...

  6. 34 CFR 300.613 - Access rights.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Access rights. 300.613 Section 300.613 Education... DISABILITIES Monitoring, Enforcement, Confidentiality, and Program Information Confidentiality of Information § 300.613 Access rights. (a) Each participating agency must permit parents to inspect and review...

  7. 10 CFR 1021.300 - General requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false General requirements. 1021.300 Section 1021.300 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NATIONAL ENVIRONMENTAL POLICY ACT IMPLEMENTING PROCEDURES Implementing Procedures § 1021.300 General requirements. (a) DOE shall determine, under the procedures in...

  8. 41 CFR 60-300.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Definitions. 60-300.2 Section 60-300.2 Public Contracts and Property Management Other Provisions Relating to Public Contracts OFFICE OF FEDERAL CONTRACT COMPLIANCE PROGRAMS, EQUAL EMPLOYMENT OPPORTUNITY, DEPARTMENT OF LABOR 300-AFFIRMATIVE ACTION AND...

  9. 33 CFR 143.300 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Applicability. 143.300 Section 143.300 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES DESIGN AND EQUIPMENT Vessels § 143.300 Applicability. This subpart applies to...

  10. 34 CFR 300.184 - Judicial review.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Judicial review. 300.184 Section 300.184 Education... DISABILITIES State Eligibility Department Procedures § 300.184 Judicial review. If a State is dissatisfied with... Court of Appeals for the circuit in which that State is located a petition for review of that...

  11. 34 CFR 300.197 - Judicial review.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Judicial review. 300.197 Section 300.197 Education... DISABILITIES State Eligibility By-Pass for Children in Private Schools § 300.197 Judicial review. If... petition for review with the United States Court of Appeals for the circuit in which the State is...

  12. 34 CFR 300.184 - Judicial review.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Judicial review. 300.184 Section 300.184 Education... DISABILITIES State Eligibility Department Procedures § 300.184 Judicial review. If a State is dissatisfied with... Court of Appeals for the circuit in which that State is located a petition for review of that...

  13. 50 CFR 300.96 - Penalties.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Penalties. 300.96 Section 300.96 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Fraser River Sockeye and Pink Salmon Fisheries § 300.96 Penalties. Any treaty Indian who commits any act...

  14. 50 CFR 300.96 - Penalties.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Penalties. 300.96 Section 300.96 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Fraser River Sockeye and Pink Salmon Fisheries § 300.96 Penalties. Any treaty Indian who commits any act...

  15. 50 CFR 300.96 - Penalties.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Penalties. 300.96 Section 300.96 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Fraser River Sockeye and Pink Salmon Fisheries § 300.96 Penalties. Any treaty Indian who commits any act...

  16. 50 CFR 300.96 - Penalties.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Penalties. 300.96 Section 300.96 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Fraser River Sockeye and Pink Salmon Fisheries § 300.96 Penalties. Any treaty Indian who commits any act...

  17. 50 CFR 300.96 - Penalties.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Penalties. 300.96 Section 300.96 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Fraser River Sockeye and Pink Salmon Fisheries § 300.96 Penalties. Any treaty Indian who commits any act...

  18. 40 CFR 300.612 - Foreign trustees.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 28 2014-07-01 2014-07-01 false Foreign trustees. 300.612 Section 300... PLAN Trustees for Natural Resources § 300.612 Foreign trustees. Pursuant to section 1006 of the OPA, foreign trustees shall act on behalf of the head of a foreign government as trustees for natural...

  19. 40 CFR 300.612 - Foreign trustees.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 29 2013-07-01 2013-07-01 false Foreign trustees. 300.612 Section 300... PLAN Trustees for Natural Resources § 300.612 Foreign trustees. Pursuant to section 1006 of the OPA, foreign trustees shall act on behalf of the head of a foreign government as trustees for natural...

  20. 40 CFR 300.612 - Foreign trustees.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 27 2010-07-01 2010-07-01 false Foreign trustees. 300.612 Section 300... PLAN Trustees for Natural Resources § 300.612 Foreign trustees. Pursuant to section 1006 of the OPA, foreign trustees shall act on behalf of the head of a foreign government as trustees for natural...

  1. 40 CFR 300.612 - Foreign trustees.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 28 2011-07-01 2011-07-01 false Foreign trustees. 300.612 Section 300... PLAN Trustees for Natural Resources § 300.612 Foreign trustees. Pursuant to section 1006 of the OPA, foreign trustees shall act on behalf of the head of a foreign government as trustees for natural...

  2. 34 CFR 300.16 - Excess costs.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Excess costs. 300.16 Section 300.16 Education... DISABILITIES General Definitions Used in This Part § 300.16 Excess costs. Excess costs means those costs that... for an example of how excess costs must be calculated.) (Authority: 20 U.S.C. 1401(8))...

  3. 34 CFR 300.230 - SEA flexibility.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... SEA, notwithstanding §§ 300.162 through 300.163 (related to State-level nonsupplanting and maintenance of effort), and § 300.175 (related to direct services by the SEA) may reduce the level of... section, an SEA may not reduce the level of expenditures described in paragraph (a) of this section if...

  4. 34 CFR 300.230 - SEA flexibility.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... SEA, notwithstanding §§ 300.162 through 300.163 (related to State-level nonsupplanting and maintenance of effort), and § 300.175 (related to direct services by the SEA) may reduce the level of... section, an SEA may not reduce the level of expenditures described in paragraph (a) of this section if...

  5. 34 CFR 300.230 - SEA flexibility.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... SEA, notwithstanding §§ 300.162 through 300.163 (related to State-level nonsupplanting and maintenance of effort), and § 300.175 (related to direct services by the SEA) may reduce the level of... section, an SEA may not reduce the level of expenditures described in paragraph (a) of this section if...

  6. 34 CFR 300.230 - SEA flexibility.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... SEA, notwithstanding §§ 300.162 through 300.163 (related to State-level nonsupplanting and maintenance of effort), and § 300.175 (related to direct services by the SEA) may reduce the level of... section, an SEA may not reduce the level of expenditures described in paragraph (a) of this section if...

  7. 21 CFR 173.300 - Chlorine dioxide.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Chlorine dioxide. 173.300 Section 173.300 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) SECONDARY DIRECT FOOD ADDITIVES PERMITTED IN FOOD FOR HUMAN CONSUMPTION Specific Usage Additives § 173.300...

  8. 49 CFR 179.300-4 - Insulation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Insulation. 179.300-4 Section 179.300-4 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY... Specifications for Multi-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-4 Insulation. (a) Tanks...

  9. 49 CFR 179.300-4 - Insulation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 3 2014-10-01 2014-10-01 false Insulation. 179.300-4 Section 179.300-4 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-4 Insulation. (a) Tanks shall not...

  10. 49 CFR 179.300-4 - Insulation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 3 2012-10-01 2012-10-01 false Insulation. 179.300-4 Section 179.300-4 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-4 Insulation. (a) Tanks shall not...

  11. 49 CFR 179.300-4 - Insulation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 3 2011-10-01 2011-10-01 false Insulation. 179.300-4 Section 179.300-4 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-4 Insulation. (a) Tanks shall not...

  12. 49 CFR 179.300-4 - Insulation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 3 2013-10-01 2013-10-01 false Insulation. 179.300-4 Section 179.300-4 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-4 Insulation. (a) Tanks shall not...

  13. 40 CFR 82.300 - Purpose.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 18 2012-07-01 2012-07-01 false Purpose. 82.300 Section 82.300 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) PROTECTION OF STRATOSPHERIC OZONE Ban on Refrigeration and Air-Conditioning Appliances Containing HCFCs § 82.300 Purpose....

  14. 40 CFR 82.300 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 17 2011-07-01 2011-07-01 false Purpose. 82.300 Section 82.300 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) PROTECTION OF STRATOSPHERIC OZONE Ban on Refrigeration and Air-Conditioning Appliances Containing HCFCs § 82.300 Purpose....

  15. 40 CFR 82.300 - Purpose.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 18 2013-07-01 2013-07-01 false Purpose. 82.300 Section 82.300 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) PROTECTION OF STRATOSPHERIC OZONE Ban on Refrigeration and Air-Conditioning Appliances Containing HCFCs § 82.300 Purpose....

  16. 40 CFR 82.300 - Purpose.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 18 2014-07-01 2014-07-01 false Purpose. 82.300 Section 82.300 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) PROTECTION OF STRATOSPHERIC OZONE Ban on Refrigeration and Air-Conditioning Appliances Containing HCFCs § 82.300 Purpose....

  17. 34 CFR 300.230 - SEA flexibility.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... SEA, notwithstanding §§ 300.162 through 300.163 (related to State-level nonsupplanting and maintenance of effort), and § 300.175 (related to direct services by the SEA) may reduce the level of... section, an SEA may not reduce the level of expenditures described in paragraph (a) of this section if...

  18. 34 CFR 300.642 - Data reporting.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Data reporting. 300.642 Section 300.642 Education... § 300.642 Data reporting. (a) Protection of personally identifiable data. The data described in section... result in disclosure of data identifiable to individual children. (b) Sampling. The Secretary may...

  19. 34 CFR 300.642 - Data reporting.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Data reporting. 300.642 Section 300.642 Education... § 300.642 Data reporting. (a) Protection of personally identifiable data. The data described in section... result in disclosure of data identifiable to individual children. (b) Sampling. The Secretary may...

  20. 34 CFR 300.642 - Data reporting.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Data reporting. 300.642 Section 300.642 Education... § 300.642 Data reporting. (a) Protection of personally identifiable data. The data described in section... result in disclosure of data identifiable to individual children. (b) Sampling. The Secretary may...

  1. 34 CFR 300.642 - Data reporting.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Data reporting. 300.642 Section 300.642 Education... § 300.642 Data reporting. (a) Protection of personally identifiable data. The data described in section... result in disclosure of data identifiable to individual children. (b) Sampling. The Secretary may...

  2. 34 CFR 300.642 - Data reporting.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Data reporting. 300.642 Section 300.642 Education... § 300.642 Data reporting. (a) Protection of personally identifiable data. The data described in section... result in disclosure of data identifiable to individual children. (b) Sampling. The Secretary may...

  3. 34 CFR 300.229 - Disciplinary information.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Disciplinary information. 300.229 Section 300.229... CHILDREN WITH DISABILITIES Local Educational Agency Eligibility § 300.229 Disciplinary information. (a) The... statement to the same extent that the disciplinary information is included in, and transmitted with,...

  4. 34 CFR 300.212 - Public information.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Public information. 300.212 Section 300.212 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND... DISABILITIES Local Educational Agency Eligibility § 300.212 Public information. The LEA must make available...

  5. 34 CFR 300.212 - Public information.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Public information. 300.212 Section 300.212 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND... DISABILITIES Local Educational Agency Eligibility § 300.212 Public information. The LEA must make available...

  6. 34 CFR 300.229 - Disciplinary information.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Disciplinary information. 300.229 Section 300.229... CHILDREN WITH DISABILITIES Local Educational Agency Eligibility § 300.229 Disciplinary information. (a) The... statement to the same extent that the disciplinary information is included in, and transmitted with,...

  7. 46 CFR 118.300 - Fire pumps.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Fire pumps. 118.300 Section 118.300 Shipping COAST GUARD... OVERNIGHT ACCOMMODATIONS FOR MORE THAN 49 PASSENGERS FIRE PROTECTION EQUIPMENT Fire Main System § 118.300 Fire pumps. (a) A self priming, power driven fire pump must be installed on each vessel. (b) On...

  8. 46 CFR 118.300 - Fire pumps.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Fire pumps. 118.300 Section 118.300 Shipping COAST GUARD... OVERNIGHT ACCOMMODATIONS FOR MORE THAN 49 PASSENGERS FIRE PROTECTION EQUIPMENT Fire Main System § 118.300 Fire pumps. (a) A self priming, power driven fire pump must be installed on each vessel. (b) On...

  9. 46 CFR 118.300 - Fire pumps.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Fire pumps. 118.300 Section 118.300 Shipping COAST GUARD... OVERNIGHT ACCOMMODATIONS FOR MORE THAN 49 PASSENGERS FIRE PROTECTION EQUIPMENT Fire Main System § 118.300 Fire pumps. (a) A self priming, power driven fire pump must be installed on each vessel. (b) On...

  10. 46 CFR 118.300 - Fire pumps.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Fire pumps. 118.300 Section 118.300 Shipping COAST GUARD... OVERNIGHT ACCOMMODATIONS FOR MORE THAN 49 PASSENGERS FIRE PROTECTION EQUIPMENT Fire Main System § 118.300 Fire pumps. (a) A self priming, power driven fire pump must be installed on each vessel. (b) On...

  11. 34 CFR 300.44 - Universal design.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Universal design. 300.44 Section 300.44 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND... DISABILITIES General Definitions Used in This Part § 300.44 Universal design. Universal design has the...

  12. 34 CFR 300.44 - Universal design.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Universal design. 300.44 Section 300.44 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND... DISABILITIES General Definitions Used in This Part § 300.44 Universal design. Universal design has the...

  13. 46 CFR 116.300 - Structural design.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Structural design. 116.300 Section 116.300 Shipping... Structure § 116.300 Structural design. Except as otherwise allowed by this subpart, a vessel must comply with the structural design requirements of one of the standards listed below for the hull material...

  14. 34 CFR 300.44 - Universal design.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Universal design. 300.44 Section 300.44 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND... DISABILITIES General Definitions Used in This Part § 300.44 Universal design. Universal design has the...

  15. 33 CFR 143.300 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Applicability. 143.300 Section 143.300 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES DESIGN AND EQUIPMENT Vessels § 143.300 Applicability. This subpart applies to all vessels engaged in OCS...

  16. 34 CFR 300.510 - Resolution process.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Resolution process. 300.510 Section 300.510 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.510 Resolution process. (a) Resolution meeting. (1) Within 15 days of receiving notice of the parent's due process...

  17. 34 CFR 300.510 - Resolution process.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Resolution process. 300.510 Section 300.510 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.510 Resolution process. (a) Resolution meeting. (1) Within 15 days of receiving notice of the parent's due process...

  18. 34 CFR 300.207 - Personnel development.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Personnel development. 300.207 Section 300.207 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND... DISABILITIES Local Educational Agency Eligibility § 300.207 Personnel development. The LEA must ensure that...

  19. 34 CFR 300.207 - Personnel development.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Personnel development. 300.207 Section 300.207 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... CHILDREN WITH DISABILITIES Local Educational Agency Eligibility § 300.207 Personnel development. The...

  20. 34 CFR 300.207 - Personnel development.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Personnel development. 300.207 Section 300.207 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND... DISABILITIES Local Educational Agency Eligibility § 300.207 Personnel development. The LEA must ensure that...

  1. 34 CFR 300.207 - Personnel development.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Personnel development. 300.207 Section 300.207 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... CHILDREN WITH DISABILITIES Local Educational Agency Eligibility § 300.207 Personnel development. The...

  2. 34 CFR 300.207 - Personnel development.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Personnel development. 300.207 Section 300.207 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... CHILDREN WITH DISABILITIES Local Educational Agency Eligibility § 300.207 Personnel development. The...

  3. 34 CFR 300.15 - Evaluation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Evaluation. 300.15 Section 300.15 Education Regulations... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES General Definitions Used in This Part § 300.15 Evaluation. Evaluation means procedures used...

  4. 34 CFR 300.122 - Evaluation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Evaluation. 300.122 Section 300.122 Education... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES State Eligibility Additional Eligibility Requirements § 300.122 Evaluation. Children...

  5. 40 CFR 158.300 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Definitions. 158.300 Section 158.300 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Product Chemistry § 158.300 Definitions. The following terms are defined for...

  6. 40 CFR 158.300 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Definitions. 158.300 Section 158.300 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Product Chemistry § 158.300 Definitions. The following terms are defined for...

  7. 40 CFR 158.300 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Definitions. 158.300 Section 158.300 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Product Chemistry § 158.300 Definitions. The following terms are defined for...

  8. 40 CFR 158.300 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 24 2011-07-01 2011-07-01 false Definitions. 158.300 Section 158.300 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Product Chemistry § 158.300 Definitions. The following terms are defined for...

  9. 50 CFR 300.104 - Scientific research.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Scientific research. 300.104 Section 300.104 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Antarctic Marine Living Resources § 300.104 Scientific research. (a) The management...

  10. 50 CFR 300.104 - Scientific research.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Scientific research. 300.104 Section 300.104 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Antarctic Marine Living Resources § 300.104 Scientific research. (a) The management...

  11. 40 CFR 158.300 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Definitions. 158.300 Section 158.300 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS DATA REQUIREMENTS FOR PESTICIDES Product Chemistry § 158.300 Definitions. The following terms are defined for the purposes of this subpart: Active...

  12. 34 CFR 300.104 - Residential placement

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Residential placement 300.104 Section 300.104 Education... DISABILITIES State Eligibility Other Fape Requirements § 300.104 Residential placement If placement in a public or private residential program is necessary to provide special education and related services to...

  13. 34 CFR 300.104 - Residential placement

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Residential placement 300.104 Section 300.104 Education... DISABILITIES State Eligibility Other Fape Requirements § 300.104 Residential placement If placement in a public or private residential program is necessary to provide special education and related services to...

  14. 11 CFR 300.34 - Transfers.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 11 Federal Elections 1 2011-01-01 2011-01-01 false Transfers. 300.34 Section 300.34 Federal Elections FEDERAL ELECTION COMMISSION BIPARTISAN CAMPAIGN REFORM ACT OF 2002-(BCRA) REGULATIONS NON-FEDERAL FUNDS State, District, and Local Party Committees and Organizations § 300.34 Transfers. (a) Federal funds. (1) Notwithstanding 11 CFR...

  15. 11 CFR 300.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 11 Federal Elections 1 2010-01-01 2010-01-01 false Definitions. 300.2 Section 300.2 Federal Elections FEDERAL ELECTION COMMISSION BIPARTISAN CAMPAIGN REFORM ACT OF 2002-(BCRA) REGULATIONS NON-FEDERAL FUNDS § 300.2 Definitions. (a) 501(c) organization that makes expenditures or disbursements in connection with a Federal election. A...

  16. 11 CFR 300.2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 11 Federal Elections 1 2011-01-01 2011-01-01 false Definitions. 300.2 Section 300.2 Federal Elections FEDERAL ELECTION COMMISSION BIPARTISAN CAMPAIGN REFORM ACT OF 2002-(BCRA) REGULATIONS NON-FEDERAL FUNDS § 300.2 Definitions. (a) 501(c) organization that makes expenditures or disbursements in connection with a Federal election. A...

  17. 50 CFR 300.109 - Gear disposal.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Gear disposal. 300.109 Section 300.109... Antarctic Marine Living Resources § 300.109 Gear disposal. (a) The operator of a harvesting vessel may not... fishing vessels or gear, or that may catch fish or cause damage to any marine resource, including...

  18. 49 CFR 179.300-7 - Materials.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 3 2013-10-01 2013-10-01 false Materials. 179.300-7 Section 179.300-7 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-7 Materials. (a) Steel plate material...

  19. 49 CFR 179.300-7 - Materials.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 3 2014-10-01 2014-10-01 false Materials. 179.300-7 Section 179.300-7 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-7 Materials. (a) Steel plate material...

  20. 46 CFR 148.300 - Radioactive materials.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... radioactive materials defined in 49 CFR 173.403 as Low Specific Activity Material, LSA-1, or Surface... 46 Shipping 5 2013-10-01 2013-10-01 false Radioactive materials. 148.300 Section 148.300 Shipping... MATERIALS THAT REQUIRE SPECIAL HANDLING Special Requirements for Certain Materials § 148.300...

  1. 49 CFR 179.300-9 - Welding.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 3 2014-10-01 2014-10-01 false Welding. 179.300-9 Section 179.300-9...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-9 Welding. (a) Longitudinal joints must be... class DOT-110A tanks. Welding procedures, welders and fabricators must be approved in accordance...

  2. 49 CFR 179.300-9 - Welding.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 3 2013-10-01 2013-10-01 false Welding. 179.300-9 Section 179.300-9...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-9 Welding. (a) Longitudinal joints must be... class DOT-110A tanks. Welding procedures, welders and fabricators must be approved in accordance...

  3. 49 CFR 179.300-9 - Welding.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 3 2012-10-01 2012-10-01 false Welding. 179.300-9 Section 179.300-9...-Unit Tank Car Tanks (Classes DOT-106A and 110AW) § 179.300-9 Welding. (a) Longitudinal joints must be... class DOT-110A tanks. Welding procedures, welders and fabricators must be approved in accordance...

  4. 33 CFR 105.300 - General.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false General. 105.300 Section 105.300 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MARITIME SECURITY MARITIME SECURITY: FACILITIES Facility Security Assessment (FSA) § 105.300 General. (a) The Facility Security Assessment (FSA) is a written document...

  5. 33 CFR 104.300 - General.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false General. 104.300 Section 104.300 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MARITIME SECURITY MARITIME SECURITY: VESSELS Vessel Security Assessment (VSA) § 104.300 General. (a) The Vessel Security Assessment (VSA) is a written document that is...

  6. 21 CFR 573.300 - Choline xanthate.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 6 2014-04-01 2014-04-01 false Choline xanthate. 573.300 Section 573.300 Food and... Listing § 573.300 Choline xanthate. Choline xanthate may be safely used as a component of animal feed as an added source of choline to supplement the diets of poultry, ruminants, and swine in...

  7. 21 CFR 573.300 - Choline xanthate.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 6 2013-04-01 2013-04-01 false Choline xanthate. 573.300 Section 573.300 Food and... Listing § 573.300 Choline xanthate. Choline xanthate may be safely used as a component of animal feed as an added source of choline to supplement the diets of poultry, ruminants, and swine in...

  8. 21 CFR 573.300 - Choline xanthate.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 6 2012-04-01 2012-04-01 false Choline xanthate. 573.300 Section 573.300 Food and... Listing § 573.300 Choline xanthate. Choline xanthate may be safely used as a component of animal feed as an added source of choline to supplement the diets of poultry, ruminants, and swine in...

  9. 7 CFR 1710.300 - General.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 11 2014-01-01 2014-01-01 false General. 1710.300 Section 1710.300 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE GENERAL AND PRE-LOAN POLICIES AND PROCEDURES COMMON TO ELECTRIC LOANS AND GUARANTEES Long-Range Financial Forecasts § 1710.300 General. Link to...

  10. 29 CFR 520.300 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 3 2014-07-01 2014-07-01 false Definitions. 520.300 Section 520.300 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR REGULATIONS EMPLOYMENT UNDER SPECIAL CERTIFICATE OF MESSENGERS, LEARNERS (INCLUDING STUDENT-LEARNERS), AND APPRENTICES Definitions § 520.300 Definitions. Administrator means...

  11. 34 CFR 300.19 - Homeless children.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Homeless children. 300.19 Section 300.19 Education... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES General Definitions Used in This Part § 300.19 Homeless children. Homeless children has...

  12. 34 CFR 300.111 - Child find.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Child find. 300.111 Section 300.111 Education... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES State Eligibility Other Fape Requirements § 300.111 Child find. (a) General. (1) The State...

  13. 34 CFR 300.111 - Child find.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Child find. 300.111 Section 300.111 Education... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES State Eligibility Other Fape Requirements § 300.111 Child find. (a) General. (1) The State...

  14. 34 CFR 300.19 - Homeless children.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Homeless children. 300.19 Section 300.19 Education... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES General Definitions Used in This Part § 300.19 Homeless children. Homeless children has...

  15. 34 CFR 300.19 - Homeless children.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Homeless children. 300.19 Section 300.19 Education... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES General Definitions Used in This Part § 300.19 Homeless children. Homeless children has...

  16. 34 CFR 300.19 - Homeless children.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Homeless children. 300.19 Section 300.19 Education... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES General Definitions Used in This Part § 300.19 Homeless children. Homeless children has...

  17. 34 CFR 300.111 - Child find.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Child find. 300.111 Section 300.111 Education... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES State Eligibility Other Fape Requirements § 300.111 Child find. (a) General. (1) The State...

  18. 34 CFR 300.111 - Child find.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Child find. 300.111 Section 300.111 Education... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES State Eligibility Other Fape Requirements § 300.111 Child find. (a) General. (1) The State...

  19. 34 CFR 300.111 - Child find.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Child find. 300.111 Section 300.111 Education... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES State Eligibility Other Fape Requirements § 300.111 Child find. (a) General. (1) The State...

  20. 34 CFR 300.19 - Homeless children.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Homeless children. 300.19 Section 300.19 Education... REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES General Definitions Used in This Part § 300.19 Homeless children. Homeless children has...

  1. 32 CFR 300.5 - General.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROGRAM DEFENSE LOGISTICS AGENCY FREEDOM OF INFORMATION ACT PROGRAM Exemptions § 300.5 General. Refer to the DoD FOIA Program regulations codified at 32 CFR part 286, subpart C, Exemptions. ... 32 National Defense 2 2014-07-01 2014-07-01 false General. 300.5 Section 300.5 National...

  2. 32 CFR 300.11 - General.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROGRAM DEFENSE LOGISTICS AGENCY FREEDOM OF INFORMATION ACT PROGRAM Fees and Fee Waivers § 300.11 General. DLA adopts the rules and rates published in 32 CFR part 286, subpart F, Fee Schedule. In addition, DLA... 32 National Defense 2 2014-07-01 2014-07-01 false General. 300.11 Section 300.11 National...

  3. 32 CFR 300.9 - Appeals.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... PROGRAM DEFENSE LOGISTICS AGENCY FREEDOM OF INFORMATION ACT PROGRAM FOIA Request Processing § 300.9... Program Regulation at 32 CFR 286.24. The appeal should be accompanied by copies of the initial request and... 32 National Defense 2 2014-07-01 2014-07-01 false Appeals. 300.9 Section 300.9 National...

  4. 32 CFR 300.10 - Judicial actions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... INFORMATION ACT PROGRAM DEFENSE LOGISTICS AGENCY FREEDOM OF INFORMATION ACT PROGRAM FOIA Request Processing § 300.10 Judicial actions. DLA adopts the DoD FOIA Program regulations codified at 32 CFR part 286... 32 National Defense 2 2014-07-01 2014-07-01 false Judicial actions. 300.10 Section 300.10...

  5. 34 CFR 300.606 - Public attention.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Public attention. 300.606 Section 300.606 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND..., and Enforcement § 300.606 Public attention. Whenever a State receives notice that the Secretary...

  6. 50 CFR 300.17 - Reporting.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... CFR 300.22); or (vii) Western Pacific Purse Seine—South Pacific Tuna Treaty Logbook (50 CFR 300.34... the time limits required by the relevant regulations: (i) Antarctic—CCAMLR Logbook (50 CFR 300.107); (ii) Atlantic—Fishing Vessel Log Reports (50 CFR 648.7(b)); (iii) Atlantic Pelagic...

  7. 50 CFR 300.21 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... aftermost hull, excluding fittings or attachments (See 46 CFR 69.203). Regional Administrator means the... America (50 CFR part 300, subpart D). Sustainable Fisheries Division (SFD) means the Assistant Regional... Eastern Pacific Tuna Fisheries § 300.21 Definitions. In addition to the terms defined in § 300.2, in...

  8. 50 CFR 300.41 - Investigation notification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Investigation notification. 300.41 Section 300.41 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.41 Investigation notification. Upon commencement of...

  9. 50 CFR 300.176 - Prohibitions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Prohibitions. 300.176 Section 300.176 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Pacific Albacore Tuna Fisheries § 300.176 Prohibitions. It is prohibited for the owner or operator of a...

  10. 50 CFR 300.41 - Investigation notification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Investigation notification. 300.41 Section 300.41 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.41 Investigation notification. Upon commencement of...

  11. 50 CFR 300.40 - Civil penalties.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Civil penalties. 300.40 Section 300.40 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.40 Civil penalties. The procedures of 15 CFR part 904 apply to...

  12. 50 CFR 300.21 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... United States of America (50 CFR part 300, subpart D). Tender vessel means a vessel that does not engage... Eastern Pacific Tuna Fisheries § 300.21 Definitions. In addition to the terms defined in § 300.2, in the Act, and in the Convention for the Establishment of an Inter-American Tropical Tuna...

  13. 50 CFR 300.21 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... United States of America (50 CFR part 300, subpart D). Tender vessel means a vessel that does not engage... Pacific Tuna Fisheries § 300.21 Definitions. In addition to the terms defined in § 300.2, in the Act, and in the Convention for the Establishment of an Inter-American Tropical Tuna Commission...

  14. 50 CFR 300.41 - Investigation notification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Investigation notification. 300.41 Section 300.41 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.41 Investigation notification. Upon commencement of...

  15. 50 CFR 300.43 - Observers.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Observers. 300.43 Section 300.43 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.43 Observers. (a) The operator and each member of the crew of a vessel...

  16. 50 CFR 300.44 - Other inspections.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Other inspections. 300.44 Section 300.44 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.44 Other inspections. The operator and each member of the crew of...

  17. 50 CFR 300.44 - Other inspections.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Other inspections. 300.44 Section 300.44 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.44 Other inspections. The operator and each member of the crew of...

  18. 50 CFR 300.173 - Vessel identification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Vessel identification. 300.173 Section 300.173 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Pacific Albacore Tuna Fisheries § 300.173 Vessel identification. Each U.S. vessel fishing...

  19. 50 CFR 300.21 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... United States of America (50 CFR part 300, subpart D). Tender vessel means a vessel that does not engage... Eastern Pacific Tuna Fisheries § 300.21 Definitions. In addition to the terms defined in § 300.2, in the Act, and in the Convention for the Establishment of an Inter-American Tropical Tuna...

  20. 50 CFR 300.176 - Prohibitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Prohibitions. 300.176 Section 300.176 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Pacific Albacore Tuna Fisheries § 300.176 Prohibitions. It is prohibited for the owner or operator of a...

  1. 50 CFR 300.176 - Prohibitions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Prohibitions. 300.176 Section 300.176 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Pacific Albacore Tuna Fisheries § 300.176 Prohibitions. It is prohibited for the owner or operator of a...

  2. 50 CFR 300.39 - Exceptions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... South Pacific Tuna Fisheries § 300.39 Exceptions. (a) The prohibitions of § 300.38 and the licensing requirements of § 300.32 do not apply to fishing for albacore tuna by vessels using the trolling method or...

  3. 50 CFR 300.39 - Exceptions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... South Pacific Tuna Fisheries § 300.39 Exceptions. (a) The prohibitions of § 300.38 and the licensing requirements of § 300.32 do not apply to fishing for albacore tuna by vessels using the trolling method or...

  4. 50 CFR 300.37 - Radio monitoring.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Radio monitoring. 300.37 Section 300.37 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.37 Radio monitoring. The international distress frequency, 2.182...

  5. 50 CFR 300.173 - Vessel identification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Vessel identification. 300.173 Section 300.173 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Pacific Albacore Tuna Fisheries § 300.173 Vessel identification. Each U.S. vessel fishing...

  6. 50 CFR 300.40 - Civil penalties.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Civil penalties. 300.40 Section 300.40 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.40 Civil penalties. The procedures of 15 CFR part 904 apply to...

  7. 50 CFR 300.40 - Civil penalties.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Civil penalties. 300.40 Section 300.40 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.40 Civil penalties. The procedures of 15 CFR part 904 apply to...

  8. 50 CFR 300.173 - Vessel identification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Vessel identification. 300.173 Section 300.173 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Pacific Albacore Tuna Fisheries § 300.173 Vessel identification. Each U.S. vessel fishing...

  9. 50 CFR 300.43 - Observers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Observers. 300.43 Section 300.43 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.43 Observers. (a) The operator and each member of the crew of a vessel...

  10. 50 CFR 300.43 - Observers.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Observers. 300.43 Section 300.43 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.43 Observers. (a) The operator and each member of the crew of a vessel...

  11. 50 CFR 300.44 - Other inspections.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Other inspections. 300.44 Section 300.44 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.44 Other inspections. The operator and each member of the crew of...

  12. 50 CFR 300.41 - Investigation notification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Investigation notification. 300.41 Section 300.41 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.41 Investigation notification. Upon commencement of...

  13. 50 CFR 300.46 - Transshipping requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Transshipping requirements. 300.46 Section 300.46 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.46 Transshipping requirements. (a) Applicability. This...

  14. 50 CFR 300.41 - Investigation notification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Investigation notification. 300.41 Section 300.41 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.41 Investigation notification. Upon commencement of...

  15. 50 CFR 300.176 - Prohibitions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Prohibitions. 300.176 Section 300.176 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Pacific Albacore Tuna Fisheries § 300.176 Prohibitions. It is prohibited for the owner or operator of a...

  16. 50 CFR 300.40 - Civil penalties.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Civil penalties. 300.40 Section 300.40 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.40 Civil penalties. The procedures of 15 CFR part 904 apply to...

  17. 50 CFR 300.43 - Observers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Observers. 300.43 Section 300.43 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.43 Observers. (a) The operator and each member of the crew of a vessel...

  18. 50 CFR 300.40 - Civil penalties.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Civil penalties. 300.40 Section 300.40 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.40 Civil penalties. The procedures of 15 CFR part 904 apply to...

  19. 50 CFR 300.173 - Vessel identification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Vessel identification. 300.173 Section 300.173 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Pacific Albacore Tuna Fisheries § 300.173 Vessel identification. A U.S. vessel fishing under...

  20. 50 CFR 300.46 - Transshipping requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Transshipping requirements. 300.46 Section 300.46 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.46 Transshipping requirements. (a) Applicability. This...

  1. 50 CFR 300.176 - Prohibitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Prohibitions. 300.176 Section 300.176 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Pacific Albacore Tuna Fisheries § 300.176 Prohibitions. It is prohibited for the owner or operator of a...

  2. 50 CFR 300.46 - Transshipping requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Transshipping requirements. 300.46 Section 300.46 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.46 Transshipping requirements. (a) Applicability. This...

  3. 50 CFR 300.43 - Observers.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Observers. 300.43 Section 300.43 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.43 Observers. (a) The operator and each member of the crew of a vessel...

  4. 50 CFR 300.44 - Other inspections.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Other inspections. 300.44 Section 300.44 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.44 Other inspections. The operator and each member of the crew of...

  5. 50 CFR 300.37 - Radio monitoring.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Radio monitoring. 300.37 Section 300.37 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.37 Radio monitoring. The international distress frequency, 2.182...

  6. 50 CFR 300.37 - Radio monitoring.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Radio monitoring. 300.37 Section 300.37 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.37 Radio monitoring. The international distress frequency, 2.182...

  7. 50 CFR 300.21 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... United States of America (50 CFR part 300, subpart D). Tender vessel means a vessel that does not engage... Eastern Pacific Tuna Fisheries § 300.21 Definitions. In addition to the terms defined in § 300.2, in the Act, and in the Convention for the Establishment of an Inter-American Tropical Tuna...

  8. 50 CFR 300.17 - Reporting.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... CFR 300.22); or (vii) Western Pacific Purse Seine—South Pacific Tuna Treaty Logbook (50 CFR 300.34... the time limits required by the relevant regulations: (i) Antarctic—CCAMLR Logbook (50 CFR 300.107); (ii) Atlantic—Fishing Vessel Log Reports (50 CFR 648.7(b)); (iii) Atlantic Pelagic...

  9. 50 CFR 300.39 - Exceptions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... South Pacific Tuna Fisheries § 300.39 Exceptions. (a) The prohibitions of § 300.38 and the licensing requirements of § 300.32 do not apply to fishing for albacore tuna by vessels using the trolling method or...

  10. 50 CFR 300.44 - Other inspections.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Other inspections. 300.44 Section 300.44 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.44 Other inspections. The operator and each member of the crew of...

  11. 50 CFR 300.46 - Transshipping requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Transshipping requirements. 300.46 Section 300.46 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.46 Transshipping requirements. (a) Applicability. This...

  12. 50 CFR 300.173 - Vessel identification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Vessel identification. 300.173 Section 300.173 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Pacific Albacore Tuna Fisheries § 300.173 Vessel identification. A U.S. vessel fishing under...

  13. 50 CFR 300.32 - Vessel licenses.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Vessel licenses. 300.32 Section 300.32 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.32 Vessel licenses. (a) Each vessel fishing in the Licensing...

  14. 50 CFR 300.37 - Radio monitoring.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Radio monitoring. 300.37 Section 300.37 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.37 Radio monitoring. The international distress frequency, 2.182...

  15. 50 CFR 300.46 - Transshipping requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Transshipping requirements. 300.46 Section 300.46 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.46 Transshipping requirements. (a) Applicability. This...

  16. 50 CFR 300.17 - Reporting.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... CFR 300.22); or (vii) Western Pacific Purse Seine—South Pacific Tuna Treaty Logbook (50 CFR 300.34... the time limits required by the relevant regulations: (i) Antarctic—CCAMLR Logbook (50 CFR 300.107); (ii) Atlantic—Fishing Vessel Log Reports (50 CFR 648.7(b)); (iii) Atlantic Pelagic...

  17. 50 CFR 300.37 - Radio monitoring.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Radio monitoring. 300.37 Section 300.37 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS South Pacific Tuna Fisheries § 300.37 Radio monitoring. The international distress frequency, 2.182...

  18. 50 CFR 300.129 - Fishing year.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 11 2012-10-01 2012-10-01 false Fishing year. 300.129 Section 300.129 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.129 Fishing year. The fishing...

  19. 50 CFR 300.129 - Fishing year.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 11 2014-10-01 2014-10-01 false Fishing year. 300.129 Section 300.129 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.129 Fishing year. The fishing...

  20. 50 CFR 300.129 - Fishing year.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Fishing year. 300.129 Section 300.129 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.129 Fishing year. The fishing...