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Sample records for 2004-5 southern brazil

  1. Methodology of the Pelotas birth cohort study from 1982 to 2004-5, Southern Brazil

    PubMed Central

    Barros, Fernando C; Victora, Cesar G; Horta, Bernardo L; Gigante, Denise P

    2009-01-01

    OBJECTIVE: To describe the methods employed in the longitudinal and follow-up studies of children born in Pelotas (Southern Brazil) in 1982. METHODS: The cohort began with a perinatal health survey that included all 6,011 children born in maternity wards in Pelotas in 1982. The 5,914 children born alive in the city were included in the follow-up studies. By 2004-5, we had conducted eight follow-ups, which consisted of the administration of questionnaires to mothers and/or cohort members, depending on age, in addition to anthropometric and clinical examination. Cohort subjects are described in terms of demographic, socioeconomic, and health-related variables collected during early follow-up studies, which are used as exposure variables. RESULTS: The majority of subjects in the cohort were followed for 23 years and on multiple occasions. The most successful follow-ups were those preceded by a city census. Using this method, we were able to locate 87.2% of subjects in 1984 (mean age 19 months), 84.1% in 1986 (mean age 43 months), and 77.4% in 2004-5, and 77.4% in 2004-5 (mean age 23 years). CONCLUSIONS: Birth cohort studies can be carried out successfully in developing countries, and the methods employed in this life-cycle study have allowed us to investigate the influence of early exposures in determining disease outcomes in adult life. PMID:19142340

  2. Education and work in the Pelotas birth cohort from 1982 to 2004-5, Southern Brazil

    PubMed Central

    Barros, Fernando C; Gigante, Denise P; Horta, Bernardo L; Victora, Cesar G

    2009-01-01

    OBJECTIVE: To analyze the influence of biological and socioeconomic factors throughout life on entry into the university and insertion in the work force of young adults from the 1982 birth cohort. METHODS: Longitudinal study of 5,914 births that took place in the city of Pelotas, Southern Brazil, in 1982. Data was collected by means of questionnaires applied on young adults when accompanying the 1982 cohort in 2004-5. Information was gathered concerning educational level and insertion in the labor market. Poisson Regression was utilized to study the effect of demographic and socioeconomic variables, as well as birth weight and maternal breastfeeding, on the outcomes. RESULTS: On the average, these young adults had 9.4 (± 3.1) years of schooling and 42% of them were attending school in 2004-5. One in five young adults had entered a university and approximately two thirds were working during the month prior to the interview. Entry in the university was determined by economic conditions. Furthermore, women's birth weight and breastfeeding among men influenced this outcome. Insertion in the labor market was more frequent among the poorer men, but this did not affect women's outcomes in this respect. CONCLUSIONS: The low inclusion in the university and the need to enter the labor market among the poor families maintains a vicious circle that reproduces the dominant social hierarchy. PMID:19142341

  3. Maternity and paternity in the Pelotas birth cohort from 1982 to 2004-5, Southern Brazil

    PubMed Central

    Gigante, Denise P; Barros, Fernando C; Veleda, Rosângela; Gonçalves, Helen; Horta, Bernardo L; Victora, Cesar G

    2009-01-01

    OBJECTIVE To describe the prevalence of maternity and paternity among subjects and its association with perinatal, socioeconomic and demographic variables. METHODS The participants were youth, aged 23, on the average, accompanied in a cohort study since they were born, in 1982, in Pelotas (Southern Brazil) and interviewed in 2004-5. Those who were considered eligible referred having had one or more children, whether these were liveborns or stillborns. Data was collected on reproductive health as well as socioeconomic and demographic information, by means of two different instruments. The independent variables were sex and skin color, family income in 1982 and in 2004-5, changes in income, birth weight and educational level when aged 23 years old. Crude and adjusted analysis were conducted by means of Poisson regression so as to investigate the effects of the independent variables on maternity/paternity during adolescence. RESULTS Among the 4,297 youth interviewed, 1,373 (32%) were parents and 842 (19.6%) of these had experienced maternity/paternity during their adolescence. Planned pregnancy of the first child was directly related to the youth’s age. Socioeconomic variables were inversely related to the occurrence of maternity/paternity during adolescence. The probability of being an adolescent mother was higher among black and mixed skin colored women, but skin color was not associated to adolescent paternity. CONCLUSIONS There was a strong relation between adolescent maternity/paternity and socioeconomic conditions, which should be taken into consideration when delineating preventive actions in the field of public health. PMID:19142344

  4. Determinants of early sexual initiation in the Pelotas birth cohort from 1982 to 2004-5, Southern Brazil

    PubMed Central

    Gonçalves, Helen; Béhague, Dominique P; Gigante, Denise P; Minten, Gicele C; Horta, Bernardo L; Victora, Cesar G; Barros, Fernando C

    2009-01-01

    OBJECTIVE To analyze social determinants of early sexual initiation among young adults from a birth cohort. METHODS Individuals from the 1982 birth cohort (N=4,297) were interviewed in 2004-5, city of Pelotas, Southern Brazil. Early sexual initiation (≤13 years of age) was the outcome. Descriptive and stratified analyses were performed according to sex. Variables analyzed were family income in 1982, ethnicity, young adult’s level of education and change in income (between 1982 and 2004-5). Ethnographic data were used to complement result analysis. RESULTS Prevalence of early sexual initiation was higher among black and mixed men, and those with low level of education and low family income in 1982 and 2004-5. More traditional male sexual role requirements, such as virility and sexual initiative, showed more repercussion and adherence from an early age among men. Young family women with higher income and level of education tended to delay their sexual initiation. Imposition of traditional values was found to influence early sexual initiation among men and women with lower level of education and income. CONCLUSIONS Results found re-established the economic factor as a determinant of behavior or uses of sexuality for both sexes. To focus on political efforts that help the economically disadvantaged to have opportunities and egalitarian future perspectives is an important strategy for health outcomes. PMID:19142343

  5. Pattern of health services utilization by adults of the Pelotas birth cohort from 1982 to 2004-5, Southern Brazil

    PubMed Central

    Dias-da-Costa, Juvenal S; Gigante, Denise P; Horta, Bernardo L; Barros, Fernando C; Victora, Cesar G

    2009-01-01

    OBJECTIVE To describe the pattern of health services utilization by young adults. METHODS Longitudinal study in Pelotas (Southern Brazil), in which the individuals were identified at birth in 1982 and followed up until 23 years of age. The outcome was defined by information collected about visits to health professionals that were attended in the year before the interview, between 2004 and 2005. The places where the visits occurred were categorized as public, private or belonging to health plan systems. Descriptive analyses were carried out for utilization and type of health service. Poisson Regression was employed in the adjusted analysis. RESULTS Of the interviewees, 72.0% visited health professionals in the year before the interview; 86.2% (95% CI 84.7;87.7) of the women and 59.3% (95% CI 57.3;61.3) of the men. Even when gynecological visits were excluded, the women still attended more visits than the men, 68.4% (95% CI 66.4;70.4). Health services utilization was more frequent among interviewees of better socioeconomic level. A difference of lower use in relation to non-white skin color was observed only among male youths. There were differences regarding the type of professional visited by men and women and also according to family income. Men and women used more frequently the public system, the health plan system and, in a smaller proportion, the private system. CONCLUSIONS The socioeconomic situation influenced the utilization and the type of health service, with men and women classified as “poor at the moment”, which indicates lower utilization of services. Such socioeconomic differences may indicate difficulties in the access to the health system. PMID:19142345

  6. Equine salmonellosis in southern Brazil.

    PubMed

    Juffo, Gregory Duarte; Bassuino, Daniele Mariath; Gomes, Danilo Carloto; Wurster, Fabiana; Pissetti, Caroline; Pavarini, Saulo Petinatti; Driemeier, David

    2017-03-01

    The Salmonella sp. genus is identified in several species, and the zoonosis it causes is one of the most important types worldwide. The specifics of salmonellosis vary according to the function of the serovar involved, the species affected, age and predisposing factors. However, few cases of equine salmonellosis have been reported. This study presents ten confirmed salmonellosis cases in equines in southern Brazil. Six were adult animals with stress factors preceding the disease, while four were foals, three of which presented with hyperacute manifestations. The main clinical signs were diarrhea, anorexia, and hyperthermia. Lesions varied in distribution and severity, although fibrinonecrotic or necrohemorrhagic enteritis was observed in all animals, mainly in the large intestine (large colon and cecum-8/10) and small intestine (3/10). Substantial liquid content, mainly hemorrhagic, was observed in all animals. The most characteristic microscopic lesion was mucosa necrosis, which is often accompanied by fibrin deposition, followed by necrosis of follicular centers and vascular changes. Bacterial isolation revealed seven isolates. Five were serotyped, and the serovars Typhimurium and Anatum were associated with two cases each, while Muenster was associated with a case whose lesion pattern varied. Immunohistochemical staining was positive in all cases. All diagnoses were based on the clinical history, macroscopic and histological lesions, and the bacterial isolation and/or immunostaining associated with histological lesions.

  7. Cystic echinococcosis in southern Brazil.

    PubMed

    de la Rue, Mario L

    2008-01-01

    Cystic echinococcosis (CE) is very common in the southern part of Rio Grande do Sul State where sheep and cattle raising is the most important economical activity. Prevalence in intermediate hosts is well known due to reports in slaughterhouses while human reports are of discussed value. This is due to underreporting of cases and few epidemiological studies make it difficult to assess the situation of echinococcosis in the population. Whereas cattle infection rate is more or less stable around 12% of the slaughter animals, in sheep there has been an increasing number in the last five years. Some efforts have been done to control the zoonosis but no effective results were obtained so far. Probably educational efforts to change human practices (feeding dogs with raw viscera), periodic treatment of dogs with praziquantel and joined actions with slaughterhouses could bring more attention to improve some control measures.

  8. Brazil to Join the European Southern Observatory

    NASA Astrophysics Data System (ADS)

    2010-12-01

    The Federative Republic of Brazil has yesterday signed the formal accession agreement paving the way for it to become a Member State of the European Southern Observatory (ESO). Following government ratification Brazil will become the fifteenth Member State and the first from outside Europe. On 29 December 2010, at a ceremony in Brasilia, the Brazilian Minister of Science and Technology, Sergio Machado Rezende and the ESO Director General, Tim de Zeeuw signed the formal accession agreement aiming to make Brazil a Member State of the European Southern Observatory. Brazil will become the fifteen Member State and the first from outside Europe. Since the agreement means accession to an international convention, the agreement must now be submitted to the Brazilian Parliament for ratification [1]. The signing of the agreement followed the unanimous approval by the ESO Council during an extraordinary meeting on 21 December 2010. "Joining ESO will give new impetus to the development of science, technology and innovation in Brazil as part of the considerable efforts our government is making to keep the country advancing in these strategic areas," says Rezende. The European Southern Observatory has a long history of successful involvement with South America, ever since Chile was selected as the best site for its observatories in 1963. Until now, however, no non-European country has joined ESO as a Member State. "The membership of Brazil will give the vibrant Brazilian astronomical community full access to the most productive observatory in the world and open up opportunities for Brazilian high-tech industry to contribute to the European Extremely Large Telescope project. It will also bring new resources and skills to the organisation at the right time for them to make a major contribution to this exciting project," adds ESO Director General, Tim de Zeeuw. The European Extremely Large Telescope (E-ELT) telescope design phase was recently completed and a major review was

  9. [Toxicity and apple production in southern Brazil].

    PubMed

    Klanovicz, Jó

    2010-03-01

    The article explores the links between the controversial apprehension of contaminated apples in southern Brazil in 1989 and the reactions of the apple industry to press reports on the use of pesticides in Brazilian orchards. The issue is framed within a broader analysis of the notions of toxicity and 'danger' surrounding the consumption of healthier food and the idea of 'food security,' notions that have begun taking hold in public and private life. It is argued that apple growers' responses to the problem can be better understood through a historical reading of the interactions between the biology of the apple tree, the agroecology of this monoculture, and the structures, actors, and discourses of the human and non-human groups in Brazil's apple-producing region.

  10. Neurotuberculosis in cattle in southern Brazil.

    PubMed

    Konradt, Guilherme; Bassuino, Daniele Mariath; Bianchi, Matheus Viezzer; Bandinelli, Marcele Bettim; Driemeier, David; Pavarini, Saulo Petinatti

    2016-06-01

    Tuberculosis in cattle is a chronic infectious-contagious disease characterized by the development of nodular lesions (granulomas) in mainly the lungs and regional lymph nodes. It is caused by Mycobacterium tuberculosis complex, an acid-fast bacillus (AFB). Tuberculosis in the central nervous system is a rare condition in cattle. Herein, we describe the clinical and pathological findings of six neurotuberculosis cases in cattle diagnosed in Southern Brazil. The average age of the cattle affected was 12 months, and they varied in breed and sex. The clinical history ranged from 5 to 30 days and was characterized by motor incoordination, opisthotonus, blindness, and progression to recumbency. The cattle were euthanized, and grossly, the leptomeninges at the basilar brain showed marked and diffuse expansion, with nodular yellowish lesions ranging in size. On microscopic examination, there were multifocal granulomas located mainly in the meninges, though sometimes extending to adjacent neuropil or existing as isolated granulomas in neuropil. AFBs were observed in the cytoplasm of epithelioid macrophages and multinucleated giant cells through Ziehl-Neelsen histochemical staining and identified as Mycobacterium sp. through immunohistochemistry.

  11. New Sycoracinae (Diptera, Psychodidae) from southern Brazil.

    PubMed

    Dos Santos, Claudiney Biral; Bravo, Freddy; Falqueto, Aloísio

    2013-01-31

    Two new species of Sycorax from the Atlantic Rain Forest of Espírito Santo, Sycorax canaanensis Santos, Bravo & Falqueto sp. nov. and Sycorax tuberculata Santos, Bravo & Falqueto sp. nov. are described and illustrated. Male speci-mens were collected with CDC light traps in the Biological Reserve of Augusto Ruschi, municipality of Santa Teresa, state of Espírito Santo, Brazil. This finding raises the number of described Western Hemisphere Sycorax species to 15.

  12. A New species and records of Gripopterygidae (Plecoptera) from Rio Grande do Sul State, Southern Brazil.

    PubMed

    Novaes, Marcos Carneiro; Da Conceição Bispo, Pitágoras

    2016-10-17

    Specimens of Gripopterygidae (Plecoptera) from Rio Grande do Sul State, southern Brazil were studied. A new species, Tupiperla sepeensis n. sp. is described. Tupiperla misionera Froehlich 2002 is a new record for Brazil and Gripopteryx reticulata Brauer 1866 and Tupiperla tessellata Brauer 1866 are new records for southern Brazil.

  13. Cryptococcus gattii-Induced Infections in Dogs from Southern Brazil.

    PubMed

    Headley, Selwyn Arlington; Di Santis, Giovana Wingeter; de Alcântara, Brígida Kussumoto; Costa, Thais Correa; da Silva, Elisangela Olegário; Pretto-Giordano, Lucienne Garcia; Gomes, Lucas Alécio; Alfieri, Amauri Alcindo; Bracarense, Ana Paula Frederico R L

    2015-10-01

    Cryptococcus gattii-induced cryptococcosis is an emerging infectious disease of humans and animals worldwide, with rare descriptions of this infection in domestic animals from Brazil. This study presents the findings associated with C. gattii in dogs from Londrina, Paraná, Southern Brazil. Two dogs, a 3-year-old, female German shepherd and a 6-year-old, male Boxer, were evaluated by a combination of pathological, mycological, and molecular diagnostic techniques. Significant pathological alterations included cryptococcal lymphadenitis, meningoencephalitis, tonsillitis, and rhinitis with nasal cryptococcomas in the German shepherd dog, while cryptococcal lymphadenitis and pneumonia were observed in the Boxer; both dogs had pseudocystic cryptococcosis. The mucicarmine histochemical stain readily identified the intralesional cryptococcal budding organisms in all affected tissues. Mycological culture and isolation confirmed the yeasts as C. gattii due to positive reaction with the L-canavanine glycine bromothymol blue agar. A PCR assay using the internal transcribed spacers (ITS)1 and ITS2 primers, which target the ITS1 and 2 regions including the 5.8S rRNA gene, amplified the desired amplicons; direct sequencing confirmed the isolate as C. gattii. ITS nucleotide differentiation demonstrated that the isolate forms part of the ITS type 4 Cryptococcus organisms which corresponds to the C. gattii VGII molecular subtype or the RAPD type 2 Cryptococcus organisms. Collectively, these findings confirmed the participation of C. gattii in the etiopathogenesis of the lesions observed in these dogs and expanded the epidemiological niche of this important mycotic agent to include Southern Brazil. It is noteworthy to mention that previous epidemiological studies have suggested that C. gattii-induced cryptococcosis is more frequently diagnosed in Northern relative to Southern Brazil, so these findings might suggest an expansion of the distribution of this agent within continental

  14. Serum antileptospiral agglutinins in freshwater turtles from Southern Brazil

    PubMed Central

    Silva, Éverton F; Seyffert, Núbia; Cerqueira, Gustavo M.; Leihs, Karl P.; Athanazio, Daniel A.; Valente, Ana L. S.; Dellagostin, Odir A.; Brod, Claudiomar S.

    2009-01-01

    In this study, we observed the presence of antileptospiral agglutinins in freshwater turtles of two urban lakes of Pelotas, Southern Brazil. Forty animals (29 Trachemys dorbigny and 11 Phrynops hilarii) were captured and studied. Attempts to isolate leptospires from blood and urine samples were unsuccessful. Serum samples (titer > 100) reactive to pathogenic strains were observed in 11 animals. These data encourage surveys of pet turtles to evaluate the risk of transmission of pathogenic leptospires to humans. PMID:24031348

  15. Ultraviolet Index measurements in Southern Bahia, Brazil

    NASA Astrophysics Data System (ADS)

    de Paula Corrêa, Marcelo; Morégula, Andréa; Fausto, Agnes; Okuno, Emico; Mol, Anderson; Santos, João C.

    2009-03-01

    This work presents results of the Joint Project for Solar UV Index Measurements in Southern Bahia State (PIU Project), and represents the first effort to measure UV radiation in this tourist region. The project goal is twofold: it has a scientific component in which the distribution of UV Index will be mapped out, and it also intends to disseminate the data as a mean of divulgation of the UV Index levels to the population. This constitutes a meaningful effort to reduce health risks from excessive solar radiation exposure in a country that reports more than 120,000 new skin cancer cases each year. This project is within the framework of a larger initiative to studying UV index distribution in Brazilian regions. PIU project has had two phases: 1) seasonal measurements were performed during the summer and winter seasons of 2006 and 2007 on a sandy beach in Ilhéus (15,0° S; 39,0° W; sea level), as well as on an asphalt surface in the urban perimeter of Itabuna (14,8° S; 39,3° W; 54 m ASL); and 2) since October 2007, measurements have been continuously conducted on a concrete surface, about 20 km from the beach, in a urban area of Ilhéus. During the summer season, UV Index reaches extreme values (>11), and in winter, results range between high (6

  16. Depressive symptoms in crack and inhalant users in Southern Brazil.

    PubMed

    Zubaran, Carlos; Foresti, Katia; Thorell, Mariana Rossi; Franceschini, Paulo; Homero, Wagner

    2010-01-01

    In this study the authors investigated depressive symptoms and correlated psychopathology in crack cocaine and inhalant users in Southern Brazil. Participants completed the Beck Depression Inventory, the Montgomery-Asberg Depression Rating Scale, and the Hamilton Depression Rating Scale. General psychopathology, hopelessness and suicidal tendencies were also investigated. Crack and inhalants users presented significant levels of depressive symptoms and psychiatric morbidity. These disorders were significantly associated with hopelessness and suicidal tendencies. These findings underscore the need for more rigorous and extensive screening efforts to detect and treat depressive symptoms in crack and inhalent users.

  17. A New Species of Culex (Melanoconion) from Southern Brazil (Diptera: Culicidae)

    DTIC Science & Technology

    1979-01-01

    and W. L. Jakob3 ABSTRACT. Cdex (Mehnoconion) Zopesi, a new species from Municipio Iguape, Estado SBo Paulo, southern Brazil is recognized. The...CostZZo do Eugenho, Municipio Iguape, in SZo Paulo state, southern Brazil in February and March, 1976. This material was found to contain specimens of

  18. Schistosomiasis in Southern Brazil 17 years after the confirmation of the first autochthonous case.

    PubMed

    Martins, Demetrius da Silva; Xavier, Mariana Fabris; Masiero, Franciéle de Souza; Cordeiro, Juliana; Thyssen, Patrícia Jacqueline

    2015-01-01

    Schistosomiasis, a parasitic disease, is an important public health issue in Brazil, particularly Northern Brazil. Since the first recorded occurrence of Biomphalaria glabrata in the States of Santa Catarina and Rio Grande do Sul, it has spread to South Brazil. Using the Information System for Notifiable Diseases (SINAN), we assessed the disease spread in Southern Brazil. In Rio Grande do Sul, nine localities had confirmed cases (n = 95, 2001-2013). We confirmed disease expansion to Southern Brazil. We demonstrated the effectiveness of SINAN to monitor notifiable diseases. Our results are useful to develop preventive actions for schistosomiasis control.

  19. Net Exchange Ecossistem in Subtropical Agriculture Area in Southern Brazil

    NASA Astrophysics Data System (ADS)

    Roberti, D. R.; Diaz, M.; Webler, G.; Fiorin, J.; de Moraes, O. L. L.; Teichrieb, C.; Amado, T.

    2015-12-01

    Southern Brazil contribute to 38% of Brazilian grain production. In contrast with the rest of the country, the south has a wet, subtropical climate that permits two annual harvests (double cropping system). The soybean and/or maize (summer) and black oat and/or wheat (winter) succession is widely used by farmers in plateau areas. In river natural lowlands, the cultivation of flooded irrigated rice is common. Changes in the land use affect the carbon, water and energy balance, and crop management practices, such as fertilization, water management, harvest and crop residues have influence in carbon exchange between the crop field and the atmosphere. This study quantifies the net exchange ecosystem (NEE) between the atmosphere and the crop cultivations in this wide region of Brazil from 2010 to 2014. We use data from two micrometeorological sites: Cruz Alta, with crop rotation and Cachoeira do Sul, with rice paddy. The carbon flux was analyzed using the eddy covariance method and gap filling procedures. The annual integration of data carbon demonstrates that the agroecosystems in southern Brazil is a acting as an light atmospheric CO2 sink. However, the NEE emissions that occurred in the fallow periods contributed negatively for such annual accumulation. To reduce this loss of CO2, farmers could cultivate plants in fallow periods, because there are favorable weather conditions for growing plants year round. Additionally, other management practices can increase the influx of C, including the production of more dry matter with cover crops by improving management and the immediate harvesting of crop after physiological maturity to reduce the period between maturation and harvest.

  20. Prevalence of wheezing in the chest among adults from the 1982 Pelotas birth cohort, Southern Brazil

    PubMed Central

    Menezes, Ana M B; Lima, Rosângela C; Minten, Gicele C; Hallal, Pedro C; Victora, Cesar G; Horta, Bernardo L; Gigante, Denise P; Barros, Fernando C

    2009-01-01

    OBJECTIVE: To estimate the prevalence of wheezing in the chest among adults, and to explore the effect of some variables on the prevalence of this condition. METHODS: This was a prospective cohort study on individuals born in the city of Pelotas (Southern Brazil) in 1982. A total of 4,297 subjects was traced in 2004-5, representing 77.4% of the original cohort. Data were collected by means of interviews using the ISAAC (International Study of Asthma and Allergies in Childhood Steering Committee) questionnaire. Associations between the outcome “occurrence of wheezing in the chest within the 12 months prior to the interview” and the variables of socioeconomic, demographic and birth characteristics were tested by means of multivariable analyses, using Poisson regression. RESULTS: The prevalence of wheezing over the preceding year was 24.9%. Among the individuals reporting wheezing, 54.6% reported difficulty in sleeping, and 12.9% reported difficulty in speaking due to wheezing. The prevalence of wheezing in the chest was significantly higher among women. This association was maintained in analyses adjusted for non-white skin color, family history of asthma and low socioeconomic level. Among men, there was no significant association in the analyses adjusted for skin color and family income at birth. Family histories of asthma and poverty throughout life presented significant associations with wheezing in the chest. For both sexes, there were no associations with the variables of birth weight and breastfeeding duration. CONCLUSIONS: The prevalence of wheezing in the chest was high, and subjects with low family income at birth were more likely to have had wheezing in the chest over the preceding year. PMID:19142351

  1. Extreme wet events in Southern Brazil during the Heinrich Stadials

    NASA Astrophysics Data System (ADS)

    Wang, X.; Rao, Z.; Auler, A.; Cheng, H.; Cruz, F. W.; Edwards, R.

    2011-12-01

    We are making continuous efforts to improve both the data resolution and chronology of the speleothem d18O record from Botuvera Cave, southern Brazil, one of the very few high-precision and high-resolution records from southern tropical continents covering the last ~ 100 kyr. In this study, we particularly focus on precipitation change in southern Brazil during the extreme cold time intervals in the North Atlantic, the so-called Heinrich (H) Stadials. With precise U/Th dating, high-resolution d18O profiles from multiple Botuvera stalagmites show that the H2, H3 and H4 stadials are about 1,000 years long and centered approximately 24.6 kyr BP, 30.0 kyr BP and 39.0 kyr BP, respectively. Following the earlier studies on Brazilian speleothem d18O records, as well as trace element analysis and modeling simulations, we interpret the calcite d18O as a proxy of monsoonal rainfall in the region. Broadly speaking, the lower calcite d18O, the higher rainfall, and vice versa. The abrupt drops on calcite d18O during these intervals, with an amplitude of ~ 2% VPDB, suggest that climate change in this region is manifested as a dramatic increasing of rainfall. This confirms our previous finding that on millennial timescales, precipitation change in southern Brazil is anti-phased with that in the north, such as eastern Asia, which is probably controlled by the meridional migration of the intertropical convergence zone (ITCZ). The fast growth rates of the samples enable us to characterize detail precipitation changes in southern Brazil during these three Heinrich Stadials. Precipitation increases abruptly in the earlier stage, evidenced by a d18O drop rate of ~ 5%/kyr, whereas during the later stage, precipitation decreases more gradually, with a d18O rising rate of ~ 2.5%/kyr. This asymmetric pattern of change is different from the temperature behavior in the North Atlantic, which warms much more dramatically in the final stage of a Heinrich Stadial than it cools in the initial

  2. Molecular characterization of encephalitic bovine listeriosis from southern Brazil.

    PubMed

    Headley, Selwyn A; Fritzen, Juliana T T; Queiroz, Gustavo R; Oliveira, Rodrigo A M; Alfieri, Alice F; Di Santis, Giovana W; Lisbôa, Júlio A N; Alfieri, Amauri A

    2014-01-01

    Reports of bovine listeriosis in Brazil are uncommon, being restricted to citations within retrospective studies, resulting in scarce documented information of this important disease of cattle. This manuscript describes the molecular findings associated with spontaneous encephalitic listeriosis in two steers from distinct herds within the state of Paraná, southern Brazil. Both animals demonstrated altered consciousness suggestive of brain stem dysfunctions and died a few days after the initial onset of disease. Polymerase chain reaction (PCR) assays were designed to target specific genes of infectious neurological agents of cattle. These included bovine herpesvirus 1 and 5 (BoHV-1 and BoHV-5), ovine herpesvirus 2 (OvHV-2), Listeria monocytogenes, and Histophilus somni. Rabies virus was discarded in evaluations done at the official state diagnostic laboratory. Gross alterations were insignificant; histopathology demonstrated rhombencephalitis associated with macrophage-predominant, multifocal to coalescing microabscesses and extensive perivascular cuffings in both steers. The L. monocytogenes PCR assay amplified the 172-bp amplicon of the listeriolysin gene from the brain stem of both animals and from the telencephalon, thalamus, and cerebellum of one of them. Phylogenetic analyses demonstrated that the strains derived from this study clustered with known strains of L. monocytogenes lineage I. The BoHV-1 and BoHV-5, OvHV-2, and H. somni PCR assays were negative. These results confirm the participation of L. monocytogenes lineage I in the etiopathogenesis of the neurological disease herein described and represent the first complete description of encephalitic listeriosis in cattle from Brazil.

  3. Traditional botanical knowledge of artisanal fishers in southern Brazil

    PubMed Central

    2013-01-01

    Background This study characterized the botanical knowledge of artisanal fishers of the Lami community, Porto Alegre, southern Brazil based on answers to the following question: Is the local botanical knowledge of the artisanal fishers of the rural-urban district of Lami still active, even since the district’s insertion into the metropolitan region of Porto Alegre? Methods This region, which contains a mosaic of urban and rural areas, hosts the Lami Biological Reserve (LBR) and a community of 13 artisanal fisher families. Semi-structured interviews were conducted with 15 fishers, complemented by participatory observation techniques and free-lists; in these interviews, the species of plants used by the community and their indicated uses were identified. Results A total of 111 species belonging to 50 families were identified. No significant differences between the diversities of native and exotic species were found. Seven use categories were reported: medicinal (49%), human food (23.2%), fishing (12.3%), condiments (8%), firewood (5%), mystical purposes (1.45%), and animal food (0.72%). The medicinal species with the highest level of agreement regarding their main uses (AMUs) were Aloe arborescens Mill., Plectranthus barbatus Andrews, Dodonaea viscosa Jacq., Plectranthus ornatus Codd, Eugenia uniflora L., and Foeniculum vulgare Mill. For illness and diseases, most plants were used for problems with the digestive system (20 species), followed by the respiratory system (16 species). This community possesses a wide botanical knowledge, especially of medicinal plants, comparable to observations made in other studies with fishing communities in coastal areas of the Atlantic Forest of Brazil. Conclusions Ethnobotanical studies in rural-urban areas contribute to preserving local knowledge and provide information that aids in conserving the remaining ecosystems in the region. PMID:23898973

  4. Monitoring cropland evapotranspiration using MODIS products in Southern Brazil

    NASA Astrophysics Data System (ADS)

    Ruhoff, Anderson; Aparecida Moreira, Adriana; de Arruda Souza, Vanessa; Roberti, Debora Regina

    2017-04-01

    Evapotranspiration (ET), including water loss from plant transpiration and land evaporation, is of vital importance for understanding hydrological processes and climate dynamics. In this context, remote sensing is considered as the most important tool for estimate ET over large areas. The Moderate Resolution Imaging Spectroradiometer (MODIS) offers an interesting opportunity to evaluate ET with spatial resolution of 1 km. The MODIS global evapotranspiration algorithm (MOD16) considers both surface energy fluxes and climatic constraints on ET (water or temperature stress) to estimate plant transpiration and soil evaporation based on Penman-Monteith equation. The algorithm is driven by remotely sensed and reanalysis meteorological data. In this study, MOD16 algorithm was applied to the State of Rio Grande do Sul (in Southern Brazil) to analyse cropland and natural vegetation evapotranspiration and its impacts during drought events. We validated MOD16 estimations using eddy correlation measurements and water balance closure at monthly and annual time scales. We used observed discharge data from three large rivers in Southern Brazil (Jacuí, Taquari and Ibicuí), precipitation data from TRMM Multi-satellite Precipitation Analysis (3B43 version 7) and terrestrial water storage estimations from the Gravity Recovery and climate Experiment (GRACE). MOD16 algorithm detected evapotranspiration in different land use and land cover conditions. In cropland areas, the average evapotranspiration was 705 mm/y, while in pasture/grassland was 750 mm/y and in forest areas was 1099 mm/y. Compared to the annual water balance, evapotranspiration was underestimated, with mean relative errors between 8 and 30% and coefficients of correlation between 0.42 to 0.53. The water storage change (dS/dt) computed from the water balance closure at monthly time scales showed a significant correlation with the terrestrial water storage obtained from GRACE data, with a coefficient of correlation of 0

  5. Factors associated to leisure-time sedentary lifestyle in adults of 1982 birth cohort, Pelotas, Southern Brazil

    PubMed Central

    Azevedo, Mario R; Horta, Bernardo L; Gigante, Denise P; Victora, Cesar G; Barros, Fernando C

    2009-01-01

    OBJECTIVE To assess factors associated to leisure-time physical activity and sedentary lifestyle. METHODS Prospective cohort study of people born in 1982 in the city of Pelotas, southern Brazil. Data were collected at birth and during in a visit in 2004-5 when 77.4% of the cohort were evaluated, making a total of 4,297 people studied. Information about leisure-time physical activity was collected using the International Physical Activity Questionnaire. Sedentary people were defined as those with weekly physical activity below 150 minutes. The following independent variables were studied: gender, skin color, birth weight, family income at birth and income change between birth and 23 years of age. Poisson’s regression with robust adjustment of variance was used for the assessment of risk factors of sedentary lifestyle. RESULTS Men reported 334 min of weekly leisure-time physical activity compared to 112 min among women. The prevalence of sedentary lifestyle was 80.6% in women and 49.2% in men. Scores of physical activity increased as income at birth increased. Those who were currently poor or who became poor during adult life were more sedentary. CONCLUSIONS Leisure-time sedentary lifestyle in young adults was high especially among women. Physical activity during leisure time is determined by current socioeconomic conditions. PMID:19142347

  6. Comparative cytogenetic analysis of marine needlefishes (Beloniformes) from southern Brazil.

    PubMed

    Cipriano, Roger Raupp; Noleto, Rafael Bueno; Kantek, Daniel Luis Zanella; da Silva Cortinhas, Maria Cristina; Cestari, Marta Margarete

    2016-08-01

    Cytogenetic studies have assisted in the taxonomic classification of organisms, especially those involving species with highly similar morphologic characteristics, or so-called cryptic species. Strongylura marina and Strongylura timucu collected from Paranaguá Bay, Paraná Coast in Southern Brazil are considered cryptic species, and the identification of interspecific variations based on the number and/or morphology of its chromosomes may serve as differentiating cytotaxonomic markers. Chromosomes of the two species were subjected to different banding and staining methods (C-, Ag-, and DAPI-CMA3), as well as chromosomal mapping of major rDNA (45S), revealed with an 18S probe by fluorescence in situ hybridization (FISH). The pattern of distribution of constitutive heterochromatin showed distinct features involving the pericentromeric and telomeric bands in both species. In S. marina, chromosome 1 represents the main species-specific marker, appearing almost entirely heterochromatic. In both species, the 45S rDNA is located at terminal region of the short arm of the chromosome 6, as detected by silver nitrate staining and FISH. Despite the apparent conserved diploid number of 48 chromosomes, data on the karyotype microstructure characterize the cytogenetic profile of the genus and may allow the establishment of cytotaxonomic and evolutionary inferences for these fishes.

  7. Toxocara spp. seroprevalence in sheep from southern Brazil.

    PubMed

    Rassier, Gabriela Lopes; Borsuk, Sibele; Pappen, Felipe; Scaini, Carlos Jaime; Gallina, Tiago; Villela, Marcos Marreiro; da Rosa Farias, Nara Amélia; Benavides, Magda Vieira; Berne, Maria Elisabeth Aires

    2013-09-01

    Visceral toxocariasis is a neglected parasitic zoonosis that occurs through the ingestion of embryonated Toxocara spp. eggs. A wide range of animal species can act as paratenic hosts for this ascarid. The main risk factor for humans is the ingestion of the eggs from contaminated soil; however, infection can also occur through the ingestion of contaminated raw or undercooked infected meat from paratenic hosts. The aim of this study was to verify the presence of Toxocara spp.-specific antibodies in sheep and to determine the risk factors associated with the infection of sheep in Rio Grande do Sul (a major sheep-producing and sheep-consuming state) in southern Brazil. Serum samples collected from 1,642 sheep were tested using an IgG enzyme-linked immunosorbent assay based on the excretory-secretory Toxocara canis antigen. Seroprevalence was 29.0% (477/1,642), and every farm included in the study contained at least one seropositive animal. These results indicate that T. canis infection is widely distributed among sheep herds in Rio Grande do Sul and that it represents a potential risk to human health.

  8. Dr. Roberto Miguel Klein Herbarium (FURB), Blumenau, Southern Brazil

    PubMed Central

    de Gasper, André Luís; Vibrans, Alexander Christian; Funez, Luís Adriano; Rigon-Jr, Morilo José; Bittencourt, Felipe; Vieira, Carina

    2014-01-01

    Abstract The premise of this study is to present the collection of the FURB herbarium, its collection area and type specimens, as well as its projects and contributions to the flora of the Subtropical Atlantic Forest. The FURB herbarium currently has nearly 41,000 records of vascular plants and has the largest collection of lycophytes and ferns in Southern Brazil, with more than 8,000 records. More than 4,500 scanned images of 4,436 species are available online, and it is expected that the whole collection will be scanned in less than one year. There are 198 families of angiosperms, 33 of ferns, three of lycophytes and six of gymnosperms. All collections of the Floristic and Forest Inventory of Santa Catarina project are recorded in FURB, which represents almost 35,000 herbarium specimens. The families with the largest number of species are: Cyperaceae (109 species), Rubiaceae (129), Solanaceae (131), Poaceae (155), Melastomataceae (157), Myrtaceae (257), Orchidaceae (288), Fabaceae (323), and Asteraceae (426), between angiosperms. Among the ferns and lycophytes are: Hymenophyllaceae (30), Thelypteridaceae (31), Aspleniaceae (32), Dryopteridaceae (43), Pteridaceae (54) and Polypodiaceae (60). There are five type specimens among them: one holotype, one isotype and three paratypes. To date, the FURB herbarium has donated 19,521 herbarium duplicates for identification or expansion of other herbaria. PMID:25383009

  9. Population Ecology of Hantavirus Rodent Hosts in Southern Brazil

    PubMed Central

    Teixeira, Bernardo R.; Loureiro, Nathalie; Strecht, Liana; Gentile, Rosana; Oliveira, Renata C.; Guterres, Alexandro; Fernandes, Jorlan; Mattos, Luciana H. B. V.; Raboni, Sonia M.; Rubio, Giselia; Bonvicino, Cibele R.; Duarte dos Santos, Claudia N.; Lemos, Elba R. S.; D'Andrea, Paulo S.

    2014-01-01

    In this study we analyze population dynamics of hantavirus rodent hosts and prevalence of infection over a 2-year period in Southern Brazil, a region with a high incidence of hantavirus pulmonary syndrome. The 14 small mammal species captured were composed of 10 rodents and four marsupials, the six most abundant species being Akodon serrensis, Oxymycterus judex, Akodon montensis, Akodon paranaensis, Oligoryzomys nigripes, and Thaptomys nigrita. These species displayed a similar pattern with increasing population sizes in fall/winter caused by recruitment and both, increase in reproductive activity and higher hantavirus prevalence in spring/summer. Specific associations between A. montensis/Jaborá Virus (JABV) and O. nigripes/Juquitiba-like Virus (JUQV-like) and spillover infections between A. paranaensis/JABV, A. serrensis/JABV, and A. paranaensis/JUQV-like were observed. Spillover infection in secondary hosts seems to play an important role in maintaining JABV and JUQV-like in the hantavirus sylvatic cycle mainly during periods of low prevalence in primary hosts. PMID:24935954

  10. Genotoxic Potential and Physicochemical Parameters of Sinos River, Southern Brazil

    PubMed Central

    Scalon, Madalena C. S.; Rechenmacher, Ciliana; Siebel, Anna Maria; Kayser, Michele L.; Rodrigues, Manoela T.; Maluf, Sharbel W.; Rodrigues, Marco Antonio S.

    2013-01-01

    The present study aimed to evaluate the physicochemical parameters and the genotoxic potential of water samples collected in the upper, middle, and lower courses of the Sinos River, southern Brazil. The comet assay was performed in the peripheral blood of fish Hyphessobrycon luetkenii exposed under laboratory conditions to water samples collected in summer and winter in three sampling sites of Sinos River. Water quality analysis demonstrated values above those described in Brazilian legislation in Parobé and Sapucaia do Sul sites, located in the middle and in the lower courses of the Sinos River, respectively. The Caraá site, located in the upper river reach, presented all the physicochemical parameters in accordance with the allowed limits in both sampling periods. Comet assay in fish revealed genotoxicity in water samples collected in the middle course site in summer and in the three sites in winter when compared to control group. Thus, the physicochemical parameters indicated that the water quality of the upper course complies with the limits set by the national guidelines, and the ecotoxicological assessment, however, indicated the presence of genotoxic agents. The present study highlights the importance of combining water physicochemical analysis and bioassays to river monitoring. PMID:24285934

  11. Fumonisins in corn (Zea mays L.) from Southern Brazil.

    PubMed

    Scussel, Vildes M; Savi, Geovana D; Costas, Lea Luzia Freitas; Xavier, José Junior Mendonça; Manfio, Daniel; Bittencourt, Karoline O; Aguiar, Kin; Stein, Stephanie M

    2014-01-01

    A total of 232 samples of corn commercialised in Santa Catarina state, Southern Brazil (temperate zone climate), were evaluated from 2007 to 2012 for fumonisins (FBs: FB1 and FB2). Before performing this study, a FBs method with liquid chromatography and fluorescence detection (ex. 335; em. 440 nm) was validated first. FBs were detected in 46.6% (108 samples), with values ranging from 66 to 7832 µg kg(-1) for FB1 and 110 to 1201 µg kg(-1) for FB2. The number of contaminated corn samples for FB1 and FB2 varied and often presented contamination of FB1 only. Per year of analysis, the numbers were: n = 22/8(FB1/FB2), 44/5(FB1/FB2), 25/12(FB1/FB2), 4(FB1), 6(FB1) and 7(FB1) in 2007, 2008, 2009, 2010, 2011 and 2012, respectively. The contamination percentage was 42.3/15.4, 59.5/6.8, 43.8/21.1, 36.4, 35.3 and 33.3%, respectively, during these years. Consumers can be exposed to these mycotoxins and their health can be at risk through the consumption of contaminated corn.

  12. Molecular epidemiology of adrenocortical tumors in southern Brazil.

    PubMed

    Custódio, Gislaine; Komechen, Heloisa; Figueiredo, Francisco R O; Fachin, Natasha D; Pianovski, Mara A D; Figueiredo, Bonald C

    2012-03-31

    The high frequency of TP53 R337H carriers in southern Brazil is responsible for the highest known incidence of childhood adrenocortical tumor (ACT). Our aims were to examine other contributing mutations, age-related risk factors, epidemiological differences in ACT and to shed light on a method for increasing the survival rate of children. The fetal zone of the adrenal cortex is believed to be one of the tissues most susceptible to adenoma or carcinoma formation due to loss of p53 function. The founder germline R337H mutation is found in 95% of ACTs of young children, a much greater proportion than in adults. Despite intense educational campaigns about the high incidence of ACT in Paraná State, advanced cases remain common. Four advanced ACT cases (4/5) were admitted to a single institution in the first 6months of 2011 in Paraná State, none of the families knew about ACT, and 2 reported no familial cancer syndrome. Curative resection is possible when a small ACT is detected early. Copyright © 2011. Published by Elsevier Ireland Ltd.

  13. Ixodidae fauna of domestic dogs in Parana, southern Brazil.

    PubMed

    Silva, Bianca Ressetti da; Garcia, Marcos Valério; Rodrigues, Vinicius da Silva; Andreotti, Renato; Dittrich, Rosangela Locatelli

    2017-01-01

    The present study aimed to contribute towards identification and registration of tick species that parasitize dogs in rural and urban areas of three mesoregions of Paraná, southern Brazil, and to estimate the rate of occurrence of each species. Fifty-six dogs with ticks living in three mesoregions: Metropolitana de Curitiba (MC), Centro Oriental (COP) and Centro Sul Paranaense (CSP), were used in the study. From these 56 dogs, 253 ticks were collected and were identified and morphologically characterized according to the species. Among all the ticks, 69.6% were identified as belonging to the species Rhipicephalus sanguineus sensu lato (s. l.); 28.1% as Amblyomma aureolatum and 2.4% as Amblyomma ovale. Among the dogs in MC that were evaluated, 57.7% were parasitized by R. sanguineus s. l., 38.5% by A. aureolatum and 3.8% by A. ovale; while in COP, 72.4% of the dogs were parasitized by A. aureolatum and 27.6% by R. sanguineus s. l.. In CSP, one tick was obtained, which was identified as A. aureolatum.

  14. [Abdominal obesity epidemiology amongst adult women resident in Southern Brazil].

    PubMed

    Olinto, Maria Teresa Anselmo; Costa, Juvenal Soares Dias da; Kac, Gilberto; Pattussi, Marcos Pascoal

    2007-12-01

    The objective of this study was to investigate the risk factors associated with abdominal obesity in women. A cross-sectional population based study was carried out on 981 women aged 20 to 60 years living in Southern Brazil. Abdominal adiposity was assessed by waist circumference (WC) = 88 cm. Poisson regression models were used to obtain prevalence ratios (PR) and their confidence intervals. The abdominal obesity prevalence was 23.3% (IC95%: 20.7-26.0). The main factors associated with the outcome were: having low education level, being unemployed, being more than 40 years old, having family obesity history, and being married. Adjusted analyses showed increased obesity prevalence in hypertensive women (Prevalence Ratio--PR = 2.06; CI95%: 1.58-2.69) and those having higher number of children (PR = 1.17; CI95% 1.00-1.37). Later menarche, at 12-13 years and at 14 years of age, protected against obesity comparing to women with earlier menarche at 8-11 years, respectively, 31% and 46% of protection. The understanding of how the abdominal obesity is distributed among the population allows effective planning and action implementation towards the reduction rates of this nutritional and public health problem.

  15. Genotoxic potential and physicochemical parameters of Sinos River, southern Brazil.

    PubMed

    Scalon, Madalena C S; Rechenmacher, Ciliana; Siebel, Anna Maria; Kayser, Michele L; Rodrigues, Manoela T; Maluf, Sharbel W; Rodrigues, Marco Antonio S; Silva, Luciano Basso da

    2013-01-01

    The present study aimed to evaluate the physicochemical parameters and the genotoxic potential of water samples collected in the upper, middle, and lower courses of the Sinos River, southern Brazil. The comet assay was performed in the peripheral blood of fish Hyphessobrycon luetkenii exposed under laboratory conditions to water samples collected in summer and winter in three sampling sites of Sinos River. Water quality analysis demonstrated values above those described in Brazilian legislation in Parobé and Sapucaia do Sul sites, located in the middle and in the lower courses of the Sinos River, respectively. The Caraá site, located in the upper river reach, presented all the physicochemical parameters in accordance with the allowed limits in both sampling periods. Comet assay in fish revealed genotoxicity in water samples collected in the middle course site in summer and in the three sites in winter when compared to control group. Thus, the physicochemical parameters indicated that the water quality of the upper course complies with the limits set by the national guidelines, and the ecotoxicological assessment, however, indicated the presence of genotoxic agents. The present study highlights the importance of combining water physicochemical analysis and bioassays to river monitoring.

  16. Environmental pathways and human exposure to manganese in southern Brazil.

    PubMed

    Hermes, Nadir; Schneider, Rosana C S; Molin, Daniela D; Riegel, Guilherme Z; Costa, Adilson B; Corbellini, Valeriano A; Torres, João P M; Malm, Olaf

    2013-01-01

    The study of environmental pathways and human exposure to Manganese (Mn) in Southern Brazil was performed using two steps. The first step consisted of taking water samples from the surface of the Pardinho River. The average results from this technique showed a significant increase of pollutants, including increased levels of Mn, above the environmentally acceptable standard recommended by the Brazilian National Environment Council. Additionally, 64 soil samples were taken from areas with and without agricultural activity. Many results were above the mean crust and did not indicate significant differences of Mn levels between the sampled areas. For the second step, 12 families were selected and assessed for exposure to Mn in a region with high levels of Mn in the soil. Most of the analyzed foods contained amounts of Mn above the reference values, indicating that food can be an important source of exposure. The Mn content from the hair of most subjects studied was also high compared to reference values from non-exposed populations. Although the contamination appeared to come from a natural origin, the results found in the present study showed that the Mn levels present in the Pardinho River Basin are a relevant public health issue.

  17. Biophysical parameters in a wheat producer region in southern Brazil

    NASA Astrophysics Data System (ADS)

    Leivas, Janice F.; de C. Teixeira, Antonio Heriberto; Andrade, Ricardo G.; de C. Victoria, Daniel; Bolfe, Edson L.; Cruz, Caroline R.

    2014-10-01

    Wheat (Triticum aestivum) is the second most produced cereal in the world, and has major importance in the global agricultural economy. Brazil is a large producer of wheat, especially the Rio Grande do Sul state, located in the south of the country. The purpose of this study was to analyze the estimation of biophysical parameters - evapotranspiration (ET), biomass (BIO) and water productivity (WP) - from satellite images of the municipalities with large areas planted with wheat in Rio Grande do Sul (RS). The evapotranspiration rate was obtained using the SAFER Model (Simple Algorithm for Retrieving Evapotranspiration) on MODIS (Moderate Resolution Imaging Spectroradiometer) images taken in the agricultural year 2012. In order to obtain biomass and water productivity rates we applied the Monteith model and the ratio between BIO and ET. In the beginning of the cycle (the planting period) we observed low values for ET, BIO and WP. During the development period, we observed an increase in the values of the parameters and decline at the end of the cycle, for the period of the wheat harvest. The SAFER model proved effective for estimating the biophysical parameters evapotranspiration, biomass production and water productivity in areas planted with wheat in Brazilian Southern. The methodology can be used for monitoring the crops' water conditions and biomass using satellite images, assisting in estimates of productivity and crop yield. The results may assist the understanding of biophysical properties of important agro-ecosystems, like wheat crop, and are important to improve the rational use of water resources.

  18. Astronomy in the Curriculum Proposals of Southern Region of Brazil

    NASA Astrophysics Data System (ADS)

    Albrecht, Evonir; Voelzke, Marcos Rincon

    2013-08-01

    Astronomy is a science that has attracted attention and fascination of different people. Because it is a subject that arouses curiosity, Astronomy has been the subject of several studies related to the area of education. In this respect, this article presents partial results of an ongoing Doctoral research. The objective is to analyze and compare the contents related to Astronomy present in curricular proposals that guide the Basic Education in Southern Brazil. The methodological approach followed the assumptions of qualitative research. We used content analysis to make the comparison between the astronomical content present in the curriculum proposals from the states of Parana, Rio Grande do Sul and Santa Catarina. The reading of the proposals has possible to reorganize the content into categories. As a result the category is displayed Earth and its analysis, which shows the lack of clarity and specificity in the presentation of the contents of the curriculum proposals. This finding may be an indicator that affect the integration of astronomical content in the curriculum of schools.

  19. Chronic low back pain among tobacco farmers in southern Brazil

    PubMed Central

    Meucci, Rodrigo D; Fassa, Anaclaudia G; Faria, Neice M X; Fiori, Nadia S

    2015-01-01

    Background: Despite tobacco farming involving intensive manual labor, chronic low back pain (CLBP) prevalence and associated factors are unknown among this occupational group. Methods: This was a cross-sectional study conducted in southern Brazil. A random sample of tobacco farmers was interviewed. Socioeconomic and individual characteristics, occupational tasks, workloads, and comorbidities were investigated. Chronic low back pain prevalence was described in relation to independent variables, and associations were examined with Poisson regression. Results: Chronic low back pain prevalence was 8·4%. Increasing age, rearing two or more species of livestock (PR 1·65), exposure to tasks that require heavy physical exertion (PR 2·00), working in awkward postures (PR 1·36), green tobacco sickness (GTS) (PR 1·63), pesticide poisoning (PR 2·37), and minor psychiatric disorders (PR 2·55) were associated with CLBP. Conclusions: This study found that CLBP is a relevant health problem among tobacco farmers and highlights understudied risk factors such as pesticide poisoning and GTS. Policies to minimize exposure to physiological and chemical workloads in tobacco planting to prevent CLBP are needed. Health professionals should be trained to diagnose and prevent acute low back pain episodes and thus prevent/minimize limitations and disabilities due to CLBP. PMID:25633930

  20. First record of Amblyomma aureolatum (Pallas, 1772) (Acari: Ixodidae) parasitizing Alouatta guariba (Humboldt) (Primata: Atelidae) in southern Brazil.

    PubMed

    Martins, João R; Salomão, Edson L; Doyle, Rovaina L; Teixeira, Mariana C; Onofrio, Valeria C; Barros-Battesti, Darci M

    2006-01-01

    A female of Amblyomma aureolatum was found on the howler monkey Alouatta guariba, in Cachoeira do Sul, Rio Grande do Sul, Southern Brazil. This is the first record of this tick species parasitizing this primate species in Brazil.

  1. Sleep deprivation and obesity in shift workers in southern Brazil.

    PubMed

    Canuto, Raquel; Pattussi, Marcos Pascoal; Macagnan, Jamile Block Araldi; Henn, Ruth Liane; Olinto, Maria Teresa Anselmo

    2014-11-01

    The objective of our study was to explore the association between sleep deprivation and obesity among shift workers. A cross-sectional study was conducted. Obesity was defined as BMI ≥30 kg/m2. Time of sleep was categorized as: >5 h of continuous sleep/d; ≤5 h of continuous sleep/d with some additional rest (sleep deprivation level I); and ≤5 h of continuous sleep/d without any additional rest (sleep deprivation level II). Sociodemographic, parental and behavioural variables were evaluated by means of a standardized pre-tested questionnaire. Potential confounding factors were controlled for in the multivariable model. A poultry-processing plant in southern Brazil. Nine hundred and five shift workers (63 % female). Obesity was more prevalent in the participants who were female, aged 40 years and older, who had less schooling and reported excess weight in both parents. Sleep deprivation levels I and II were associated with increased income, number of meals consumed throughout the day and nightshift work. All of the workers who exhibited a degree of sleep deprivation worked the night shift. After controlling for potential confounding factors, the prevalence ratios of obesity were 1·4 (95 % CI 0·8, 2·2) and 4·4 (95 % CI 2·4, 8·0) in the workers with sleep deprivation levels I and II, respectively, compared with the reference group. These results show a strong association between sleep deprivation and obesity in shift workers and that sleep deprivation may be a direct consequence of working at night.

  2. A new species of land flatworm (Platyhelminthes: Continenticola) from areas of Araucaria Forest in southern Brazil.

    PubMed

    Rossi, Ilana; Fontoura, Marcela; Amaral, Silvana; Leal-Zanchet, Ana M

    2014-05-08

    The genus Cratera Carbayo et al. was proposed to encompass five species of Geoplaninae from southeastern Brazil that were mainly recorded in the state of São Paulo. Here we describe a new species of the genus, C. steffeni sp. nov., that occurs in areas of Araucaria Forest in southern Brazil, which augments the known distribution of Cratera. The new species is distinguished from others of the genus by its characteristic colour pattern and a combination of internal morphological characters.

  3. The alkaline and alkaline-carbonatite magmatism from Southern Brazil

    NASA Astrophysics Data System (ADS)

    Ruberti, E.; Gomes, C. D. B.; Comin-Chiaramonti, P.

    2015-12-01

    Early to Late Cretaceous lasting to Paleocene alkaline magmatism from southern Brazil is found associated with major extensional structural features in and around the Paraná Basin and grouped into various provinces on the basis of several data. Magmatism is variable in size, mode of occurrence and composition. The alkaline rocks are dominantly potassic, a few occurrences showing sodic affinity. The more abundant silicate rocks are evolved undersaturated to saturated in silica syenites, displaying large variation in igneous forms. Less evolved types are restricted to subvolcanic environments and outcrops of effusive suites occur rarely. Cumulatic mafic and ultramafic rock types are very common, particularly in the alkali-carbonatitic complexes. Carbonatite bodies are represented by Ca-carbonatites and Mg-carbonatites and more scarcely by Fe-carbonatites. Available radiometric ages for the alkaline rocks fit on three main chronological groups: around 130 Ma, subcoveal with the Early Cretaceous flood tholeiites of the Paraná Basin, 100-110 Ma and 80-90 Ma (Late Cretaceous). The alkaline magmatism also extends into Paleocene times, as indicated by ages from some volcanic lavas. Geochemically, alkaline potassic and sodic rock types are distinguished by their negative and positive Nb-Ta anomalies, respectively. Negative spikes in Nb-Ta are also a feature common to the associated tholeiitic rocks. Sr-Nd-Pb systematics confirm the contribution of both HIMU and EMI mantle components in the formation of the alkaline rocks. Notably, Early and Late Cretaceous carbonatites have the same isotopic Sr-Nd initial ratios of the associated alkaline rocks. C-O isotopic Sr-Nd isotopic ratios indicate typical mantle signature for some carbonatites and the influence of post-magmatic processes in others. Immiscibility of liquids of phonolitic composition, derived from mafic alkaline parental magmas, has been responsible for the origin of the carbonatites. Close association of alkaline

  4. Factors associated with dental fluorosis in school children in southern Brazil: a cross-sectional study.

    PubMed

    Azevedo, Marina Sousa; Goettems, Marília Leão; Torriani, Dione Dias; Demarco, Flávio Fernando

    2014-01-01

    This cross-sectional study assessed risk factors for dental fluorosis (DF) among 8- to 12-year-old children in southern Brazil. Children attending 20 schools were randomly selected (n=1,196). They were interviewed and their parents answered a questionnaire that was sent home. Prevalence of DF was 8.53% (modified Dean's criteria), and the prevalence of severe DF was 0.17%. The results of multiple logistic regression analyses indicated that DF was associated with a higher frequency of tooth brushing and with initial use of fluoride toothpaste at the emergence of the first tooth. DF does not constitute a public health problem in southern Brazil.

  5. Reflections on the Impact of a Long Term Theatre for Community Development Project in Southern Brazil

    ERIC Educational Resources Information Center

    Nogueira, Marcia Pompeo

    2006-01-01

    This paper analyses a community theatre project initiated in 1991 in Ratones, a community in Southern Brazil. It began as an extension project of the State University of Santa Catarina and became an independent project coordinated by former participants, still in collaboration with the university. Aiming to evaluate the benefits of this theatre…

  6. New species of Rhagoletotrypeta (Diptera: Tephritidae) from the Dominican Republic and southern Brazil and Paraguay

    USDA-ARS?s Scientific Manuscript database

    Rhagoletotrypeta chapecensis Norrbom & Savaris, new species, and R. gelabertae Norrbom & Savaris, new species, are described and illustrated from specimens from southern Brazil (Paraná, Santa Catarina) and Paraguay, and from the Dominican Republic, respectively. The larvae of R. chapecensis develop ...

  7. Reflections on the Impact of a Long Term Theatre for Community Development Project in Southern Brazil

    ERIC Educational Resources Information Center

    Nogueira, Marcia Pompeo

    2006-01-01

    This paper analyses a community theatre project initiated in 1991 in Ratones, a community in Southern Brazil. It began as an extension project of the State University of Santa Catarina and became an independent project coordinated by former participants, still in collaboration with the university. Aiming to evaluate the benefits of this theatre…

  8. Construction of a remotely sensed area sampling frame for Southern Brazil

    NASA Technical Reports Server (NTRS)

    Fecso, R.; Gardner, W.; Hale, B.; Johnson, V.; Pavlasek, S. (Principal Investigator)

    1982-01-01

    A remotely sensed area sampling frame was constructed for selected areas in Southern Brazil. The sampling unit information was stored in digital form in a latitudinal/longitudinal characterized population. Computerized sampling procedures were developed which allow for flexibility in sample unit specifications and sampling designs.

  9. Copper Sprays and Windbreaks for Control of Citrus Canker on Young Orange Trees in Southern Brazil

    USDA-ARS?s Scientific Manuscript database

    The benefit of windbreaks and copper sprays for control of citrus canker caused by Xanthomonas axonopodis pv. citri was investigated in a commercial citrus orchard located in a citrus canker endemic area in southern Brazil. Control of canker was evaluated as incidence and severity of lesions on foli...

  10. Antimicrobial resistance profile of Enterococcus spp isolated from food in Southern Brazil

    PubMed Central

    Riboldi, Gustavo Pelicioli; Frazzon, Jeverson; d’Azevedo, Pedro Alves; Frazzon, Ana Paula Guedes

    2009-01-01

    Fifty-six Enterococcus spp. strains were isolated from foods in Southern Brazil, confirmed by PCR and classified as Enterococcus faecalis (27), Enterococcus faecium (23) and Enterococcus spp (6). Antimicrobial susceptibility tests showed resistance phenotypes to a range of antibiotics widely administrated in humans such as gentamycin, streptomycin, ampicillin and vancomycin. PMID:24031330

  11. Assignment of serotype to Salmonella enterica isolates obtained from poultry and their environment in Southern Brazil.

    USDA-ARS?s Scientific Manuscript database

    To assess diversity of Salmonella enterica serotypes present in poultry and their environment from Southern Brazil, the Kauffman-White-LeMinor (KWL) scheme was used to serotype a total of 155 isolates. Isolates were then re-examined with nested PCR and sequencing of the dkgB-linked Intergenic Sequ...

  12. Hantavirus Pulmonary Syndrome, Central Plateau, Southeastern, and Southern Brazil

    PubMed Central

    Moreli, Marcos L.; de Sousa, Ricardo L.M.; Borges, Alessandra A.; de Figueiredo, Glauciane G.; Machado, Alex M.; Bisordi, Ivani; Nagasse-Sugahara, Teresa K.; Suzuki, Akemi; Pereira, Luiz E.; de Souza, Renato P.; de Souza, Luiza T.M.; Braconi, Carla T.; Harsi, Charlotte M.; de Andrade Zanotto, Paolo M.

    2009-01-01

    Hantavirus pulmonary syndrome (HPS) is an increasing health problem in Brazil because of encroachment of sprawling urban, agricultural, and cattle-raising areas into habitats of subfamily Sigmodontinae rodents, which serve as hantavirus reservoirs. From 1993 through June 2007, a total of 884 cases of HPS were reported in Brazil (case-fatality rate 39%). To better understand this emerging disease, we collected 89 human serum samples and 68 rodent lung samples containing antibodies to hantavirus from a 2,500-km-wide area in Brazil. RNA was isolated from human samples and rodent tissues and subjected to reverse transcription–PCR. Partial sequences of nucleocapsid protein and glycoprotein genes from 22 human and 16 rodent sources indicated only Araraquara virus and Juquitiba virus lineages. The case-fatality rate of HPS was higher in the area with Araraquara virus. This virus, which may be the most virulent hantavirus in Brazil, was associated with areas that have had greater anthropogenic changes. PMID:19331732

  13. Aerial sketchmapping for monitoring forest conditions in Southern Brazil

    Treesearch

    Y. M. Malheiros de Oliveira; M. A. Doetzer Rosot; N. B. da Luz; W. M. Ciesla; E.W. Johnson; R. Rhea; J.F. Jr. Penteado

    2006-01-01

    Aerial sketchmapping is a simple, low cost remote sensing method used for detection and mapping of forest damage caused by biotic agents (insects, pathogens and other pests) and abiotic agents (wind, fire, storms, hurricane, ice storms) in North America. This method was introduced to Brazil in 2001/2002 via a USDA Forest Service/EMBRAPA technical exchange program,...

  14. Seven new microendemic species of Brachycephalus (Anura: Brachycephalidae) from southern Brazil

    PubMed Central

    Ribeiro, Luiz F.; Bornschein, Marcos R.; Belmonte-Lopes, Ricardo; Firkowski, Carina R.; Morato, Sergio A.A.

    2015-01-01

    Brachycephalus (Anura: Brachycephalidae) is a remarkable genus of miniaturized frogs of the Brazilian Atlantic Rainforest. Many of its species are highly endemic to cloud forests, being found only on one or a few mountaintops. Such level of microendemism might be caused by their climatic tolerance to a narrow set of environmental conditions found only in montane regions. This restriction severely limits the chance of discovery of new species, given the difficulty of exploring these inaccessible habitats. Following extensive fieldwork in montane areas of the southern portion of the Atlantic Rainforest, in this study we describe seven new species of Brachycephalus from the states of Paraná and Santa Catarina, southern Brazil. These species can be distinguished from one another based on coloration and the level of rugosity of the skin in different parts of their body. These discoveries increase considerably the number of described species of Brachycephalus in southern Brazil. PMID:26056613

  15. Seven new microendemic species of Brachycephalus (Anura: Brachycephalidae) from southern Brazil.

    PubMed

    Ribeiro, Luiz F; Bornschein, Marcos R; Belmonte-Lopes, Ricardo; Firkowski, Carina R; Morato, Sergio A A; Pie, Marcio R

    2015-01-01

    Brachycephalus (Anura: Brachycephalidae) is a remarkable genus of miniaturized frogs of the Brazilian Atlantic Rainforest. Many of its species are highly endemic to cloud forests, being found only on one or a few mountaintops. Such level of microendemism might be caused by their climatic tolerance to a narrow set of environmental conditions found only in montane regions. This restriction severely limits the chance of discovery of new species, given the difficulty of exploring these inaccessible habitats. Following extensive fieldwork in montane areas of the southern portion of the Atlantic Rainforest, in this study we describe seven new species of Brachycephalus from the states of Paraná and Santa Catarina, southern Brazil. These species can be distinguished from one another based on coloration and the level of rugosity of the skin in different parts of their body. These discoveries increase considerably the number of described species of Brachycephalus in southern Brazil.

  16. Verification of concentration time formulae accuracy in Southern Brazil

    NASA Astrophysics Data System (ADS)

    Freitas Ferreira, Pedro; Allasia, Daniel; Herbstrith Froemming, Gabriel; Ribeiro Fontoura, Jessica; Tassi, Rutineia

    2016-04-01

    The time of concentration (TC) of an urban catchment is a fundamental watershed parameter used to compute the peak discharge and/or in the hydrological simulation of sewer systems. In the lack of hydrological data for its estimative, several empirical formulae are used, however, almost none of them have been verified in Brazil leading to large uncertainties in the correct value. In this light, were tested several formulae such as the proposed by Kirpich (and a modifications of this equation proposed by the National Transport Bureau of Brazil (DNIT)), U.S. Corps. Of Engineers, Pasini, Dooge , Johnstone , Ventura and Ven T Chow as they are used in Brazil. The verification was accomplished against measured data in 5 sub-basins situated in the Dilúvio basin, a semi urbanized watershed that contains the most developed area of the city of Porto Alegre. All the rainfall stations were active in the period from late 1970's until early 1980's due to the existence of Projeto Dilúvio but today, however, only two of them are still in operation. Porto Alegre is the capital and largest city in the Brazilian southernmost state of Rio Grande do Sul with a population of approximately 1.6 million inhabitants, the tenth most populous city in the country and the centre of Brazil's fourth largest metropolitan area, with almost 4,5 million inhabitants (IBGE, 2010). The city is situated in a humid subtropical climate with high and regular precipitation throughout the year. Most summer rainfall occurs during thunderstorms and an occasional tropical storm, hurricane or cyclone. The results showed an error of around 70% for half of the formulas, with a tendency to underestimate TC values. Among the tested methods, Johnstone had the best overall result, with an average error of 25%, well far from the second, Dooge, with 43% of average error. The best results were obtained in only one basin, Dilúvio, the largest one, with an area of 25km², with an error of just 3% for Modified Kirpich, and

  17. Astronomy and Cosmology of the Guarani of Southern Brazil

    NASA Astrophysics Data System (ADS)

    de Mello, Flávia Cristina

    The Guarani Indians in South Brazil have a sophisticated system of thought about the cosmos. Presented here are some elements of their cosmology and cosmogony and the influences of the heavenly bodies in this people's everyday life, which have been collected in ethnographic research in the first decade of the 21st century. The main themes of cosmology and the origin of the Sun, Moon, and Earth are described, approximating this anthropological research to ethnoastronomy and cultural anthropology discussions. This research seeks to analyze comparatively this cosmological concept with other indigenous cosmological systems and to compare them with the Western cosmological system, thus including it in studies of cultural astronomy.

  18. Home range and density of three sympatric felids in the Southern Atlantic Forest, Brazil.

    PubMed

    Kasper, C B; Schneider, A; Oliveira, T G

    2016-02-01

    Home range and minimal population densities of Southern tiger cat (Leopardus guttulus), margay (Lepardus wiedii) and jaguarundi (Puma yagouaroundi) were estimated between 2005 and 2006 in Taquari Valley, near the southern edge of the Atlantic Rainforest in Brazil. Home range data were collected by conventional radio telemetry (VHF) locations in a highly fragmented landscape. The average home range size, calculated using 95% kernel density estimates, was 16.01 km2 for Southern tiger cat, 21.85 km2 for margay and 51.45 km2 for jaguarundi. Telemetry data were used to obtain minimal density estimates of 0.08 Southern tiger cats / km2, and 0.04 jaguarundi / km2. The density estimates arise from areas where ocelot (Leopardus pardalis) and other larger-bodied carnivores were locally extinct, and they suggest a specific type of mesopredator release known as the ocelot effect, which is likely enabling the increase in smaller felid populations in this area.

  19. Recent precipitation trends, flash floods and landslides in southern Brazil

    NASA Astrophysics Data System (ADS)

    Ávila, Alvaro; Justino, Flavio; Wilson, Aaron; Bromwich, David; Amorim, Marcelo

    2016-11-01

    In order to understand the rising number of flash floods and landslides in the densely populated region of southeastern Brazil, this study analyzes the spatial and temporal changes in precipitation from 1978 to 2014. We focus on the sensitivity of mountainous regions, specifically the Rio de Janeiro (RJMR) and Santa Catarina (SCMR) regions. Daily rainfall observations are aggregated into annual and seasonal indexes, and RClimdex is used to evaluate a suite of precipitation and extreme event indexes. Results show positive annual and seasonal precipitation trends during all seasons except for the winter season in the RJMR. Diverse change points in their time series, spatial differences in the trends at individual stations, and trends associated with elevation suggest that despite the close proximity of these two regions, climate impacts are not uniform across all of southeastern Brazil. The majority of precipitation-related indexes present positive trends, especially in the extreme precipitation indexes (PRCPTOT, RX1day, Rx5day and R30 mm). Statistically significant positive correlations are discovered between landslides/flash floods events and annual maximum 1-day and 5-day consecutive precipitation, and these indexes may be useful indicators of natural hazard events for this region.

  20. A new marseillevirus isolated in Southern Brazil from Limnoperna fortunei.

    PubMed

    Dos Santos, Raíssa Nunes; Campos, Fabrício Souza; Medeiros de Albuquerque, Nathalia Rammé; Finoketti, Fernando; Côrrea, Rayra Almeida; Cano-Ortiz, Lucia; Assis, Felipe Lopes; Arantes, Thalita Souza; Roehe, Paulo Michel; Franco, Ana Cláudia

    2016-10-14

    Members of the family Marseilleviridae are giant viruses that have the ability to infect amoebas. Such viruses were initially described in 2009. Since then, this family has grown, and diverse members have been found in different environments and geographic locations. Previous phylogenetic analyses suggested the existence of four marseillevirus lineages. A fourth lineage was described with the discovery of the Brazilian marseillevirus (BrMr), isolated from Pampulha Lake, Brazil. Here we describe the isolation and characterization of the Golden marseillevirus (GMar), a new marseillevirus isolated from golden mussels (Limnoperna fortunei) in South of Brazil. This new representative of Marseilleviridae has circular, double-stranded (dsDNA) that contains 360, 610 base pairs and encodes 483 open read frames (ORFs). The complete virus genome was sequenced and phylogenic analyses indicated clear differences between this virus and other marseilleviruses. In addition, this is the only marseillevirus so far that has been isolated from mussels, and this report expands the diversity of environments from which giant viruses could be recovered.

  1. A new marseillevirus isolated in Southern Brazil from Limnoperna fortunei

    PubMed Central

    dos Santos, Raíssa Nunes; Campos, Fabrício Souza; Medeiros de Albuquerque, Nathalia Rammé; Finoketti, Fernando; Côrrea, Rayra Almeida; Cano-Ortiz, Lucia; Assis, Felipe Lopes; Arantes, Thalita Souza; Roehe, Paulo Michel; Franco, Ana Cláudia

    2016-01-01

    Members of the family Marseilleviridae are giant viruses that have the ability to infect amoebas. Such viruses were initially described in 2009. Since then, this family has grown, and diverse members have been found in different environments and geographic locations. Previous phylogenetic analyses suggested the existence of four marseillevirus lineages. A fourth lineage was described with the discovery of the Brazilian marseillevirus (BrMr), isolated from Pampulha Lake, Brazil. Here we describe the isolation and characterization of the Golden marseillevirus (GMar), a new marseillevirus isolated from golden mussels (Limnoperna fortunei) in South of Brazil. This new representative of Marseilleviridae has circular, double-stranded (dsDNA) that contains 360, 610 base pairs and encodes 483 open read frames (ORFs). The complete virus genome was sequenced and phylogenic analyses indicated clear differences between this virus and other marseilleviruses. In addition, this is the only marseillevirus so far that has been isolated from mussels, and this report expands the diversity of environments from which giant viruses could be recovered. PMID:27739526

  2. Yellow Fever Virus in Haemagogus leucocelaenus and Aedes serratus Mosquitoes, Southern Brazil, 2008

    PubMed Central

    Cardoso, Jáder da C.; de Almeida, Marco A.B.; dos Santos, Edmilson; da Fonseca, Daltro F.; Sallum, Maria A.M.; Noll, Carlos A.; Monteiro, Hamilton A. de O.; Cruz, Ana C.R.; Carvalho, Valéria L.; Pinto, Eliana V.; Castro, Francisco C.; Neto, Joaquim P. Nunes; Segura, Maria N.O.

    2010-01-01

    Yellow fever virus (YFV) was isolated from Haemagogus leucocelaenus mosquitoes during an epizootic in 2001 in the Rio Grande do Sul State in southern Brazil. In October 2008, a yellow fever outbreak was reported there, with nonhuman primate deaths and human cases. This latter outbreak led to intensification of surveillance measures for early detection of YFV and support for vaccination programs. We report entomologic surveillance in 2 municipalities that recorded nonhuman primate deaths. Mosquitoes were collected at ground level, identified, and processed for virus isolation and molecular analyses. Eight YFV strains were isolated (7 from pools of Hg. leucocelaenus mosquitoes and another from Aedes serratus mosquitoes); 6 were sequenced, and they grouped in the YFV South American genotype I. The results confirmed the role of Hg. leucocelaenus mosquitoes as the main YFV vector in southern Brazil and suggest that Ae. serratus mosquitoes may have a potential role as a secondary vector. PMID:21122222

  3. Prevalence of Torque teno virus in healthy donors of Paraná State, southern Brazil

    PubMed Central

    Mazzola, Jocimara Costa; Saito, Patrícia Keiko; Yamakawa, Roger Haruki; Watanabe, Maria Angélica Ehara; da Silva Junior, Waldir Veríssimo; Matta, Alessandra Cristina Gobbi; Borelli, Sueli Donizete

    2015-01-01

    Objective To determine the prevalence of the Torque teno virus in healthy donors in the northern and northwestern regions of the state of Paraná, southern Brazil. Methods The Torque teno virus was detected by a nested polymerase chain reaction using a set of oligoprimers for the N22 region. Results The prevalence of the virus was 69% in 551 healthy blood donors in southern Brazil. There was no statistically significant difference between the presence of the virus and the variables gender, ethnicity and marital status. There was significant difference in the prevalence of the virus regarding the age of the donors (p-value = 0.024) with a higher incidence (74.7%) in 18- to 24-year-old donors. Conclusion A high prevalence of Torque teno virus was observed in the population studied. Further studies are needed to elucidate the routes of contamination and the clinical implications of the virus in the healthy population. PMID:26408369

  4. First Evaluation of an Outbreak of Bovine Babesiosis and Anaplasmosis in Southern Brazil Using Multiplex PCR

    PubMed Central

    Canever, Mariana Feltrin; Vieira, Luisa Lemos; Reck, Carolina; Richter, Luisa

    2014-01-01

    Outbreaks of tick-borne disease cases in Santa Catarina, Brazil are known, but the presence of the pathogen DNA has never been determined. In this study, the first survey of Anaplasma marginale, Babesia bigemina, and Babesia bovis DNA on blood samples of 33 cattle from an outbreak in Ponte Alta Municipality, Santa Catarina, Brazil, has been carried out. A multiplex PCR detected 54.5% of animals were co-infected with 2 or 3 parasites, while 24.2% were infected with only 1 species. The most prevalent agent was B. bigemina (63.6%) followed by A. marginale (60.6%). This is the first report of tick-borne disease pathogens obtained by DNA analysis in Southern Brazil. PMID:25352699

  5. Elevated Toxoplasma gondii Infection Rates for Retinas from Eye Banks, Southern Brazil.

    PubMed

    Commodaro, Alessandra G; Chiasson, Melissa; Sundar, Natarajan; Rizzo, Luiz Vicente; Belfort, Rubens; Grigg, Michael E

    2016-04-01

    We found significantly higher incidence of Toxoplasma gondii DNA in eye bank specimens from Joinville in southern Brazil (13/15, 87%) than in São Paulo (3/42, 7%; p = 2.1 × 10E-8). PCR DNA sequence analysis was more sensitive at locus NTS2 than at locus B1; a high frequency of mixed co-infections was detected.

  6. Metal contamination of vineyard soils in wet subtropics (southern Brazil).

    PubMed

    Mirlean, Nicolai; Roisenberg, Ari; Chies, Jaqueline O

    2007-09-01

    The vine-growing areas in Brazil are the dampest in the world. Copper maximum value registered in this study was as much as 3200 mg kg(-1), which is several times higher than reported for vineyard soils in temperate climates. Other pesticide-derived metals accumulate in the topsoil layer, surpassing in the old vineyards the background value several times for Zn, Pb, Cr and Cd. Copper is transported to deeper soils' horizons and can potentially contaminate groundwater. The soils from basaltic volcanic rocks reveal the highest values of Cu extracted with CaCl(2), demonstrating a high capacity of copper transference into plants. When evaluating the risks of copper's toxic effects in subtropics, the soils from rhyolitic volcanic rocks are more worrisome, as the Cu extracted with ammonium acetate 1M surpasses the toxic threshold as much as 4-6 times.

  7. Molecular detection of Ehrlichia canis and Anaplasma platys in dogs in Southern Brazil.

    PubMed

    Lasta, Camila Serina; dos Santos, Andrea Pires; Messick, Joanne Belle; Oliveira, Simone Tostes; Biondo, Alexander Welker; Vieira, Rafael Felipe da Costa; Dalmolin, Magnus Larruscaim; González, Félix Hilario Diaz

    2013-01-01

    The aims of this study were to determine the occurrence of Anaplasma platys and Ehrlichia canis infection in dogs in Porto Alegre, Southern Brazil; and to investigate their association with hematological abnormalities. Serum samples from 196 dogs were first tested using dot-ELISA for antibodies against Anaplasma spp. and Ehrlichia canis. Peripheral blood samples from 199 dogs were subjected to 16S rRNA nested PCR (nPCR) for A. platys and E. canis, followed by DNA sequencing to ensure pathogen identity. A total of 19/196 samples (9.69%) were positive for Anaplasma spp. using ELISA and 28/199 (14.07%) samples were positive for A. platys by nested PCR. All the dog samples were negative for E. canis, both in anti-E. canis antibody tests and in nested PCR. There were no significant differences in hematological parameters between A. platys-PCR positive and negative dogs and Anaplasma spp. serologically positive dogs, except for basophil counts, which were higher in nPCR-positive dogs. This is the first report showing A. platys presence in dogs in Southern Brazil. In conclusion, hematological parameters may not be sufficient to diagnose A. platys infection in dogs in Southern Brazil, probably due either to low pathogenicity or to chronic infection. On the other hand, E. canis may either have very low occurrence or be absent in dogs in Porto Alegre.

  8. Greenhouse gas emission associated with sugar production in southern Brazil

    PubMed Central

    2010-01-01

    Background Since sugarcane areas have increased rapidly in Brazil, the contribution of the sugarcane production, and, especially, of the sugarcane harvest system to the greenhouse gas emissions of the country is an issue of national concern. Here we analyze some data characterizing various activities of two sugarcane mills during the harvest period of 2006-2007 and quantify the carbon footprint of sugar production. Results According to our calculations, 241 kg of carbon dioxide equivalent were released to the atmosphere per a ton of sugar produced (2406 kg of carbon dioxide equivalent per a hectare of the cropped area, and 26.5 kg of carbon dioxide equivalent per a ton of sugarcane processed). The major part of the total emission (44%) resulted from residues burning; about 20% resulted from the use of synthetic fertilizers, and about 18% from fossil fuel combustion. Conclusions The results of this study suggest that the most important reduction in greenhouse gas emissions from sugarcane areas could be achieved by switching to a green harvest system, that is, to harvesting without burning. PMID:20565736

  9. Floristic evolution in an agroforestry system cultivation in Southern Brazil.

    PubMed

    Silva, Luís C R; Machado, Sebastião A; Galvão, Franklin; Figueiredo, Afonso

    2016-06-07

    Bracatinga (Mimosa scabrella Bentham) is an important pioneer tree species in Ombrophylous Mixed Forest of Brazil and is widely used as an energy source. In traditional agroforestry systems, regeneration is induced by fire, then pure and dense stands known as bracatinga stands (bracatingais) are formed. In the first year, annual crops are intercalated with the seedlings. At that time the seedlings are thinned, then the stands remain at a fallow period and cut at seven years old. The species is very important mainly for small landowners. We studied the understory species that occur naturally during the succession over several years in order to manage them rationally in the future and maintain the natural vegetation over time. Three to 20 year-old Bracatinga stands were sampled between 1998 and 2011. All tree species with diameter at breast height (DBH) ≥ 5 cm were measured.The floristic evolution was assessed with respect to Sociability Index, the Shannon Diversity Index and the Pielou Evenness Index. Graphs of rank/abundance over different age groups were evaluated using the Kolmogorov-Smirnov test. We identified 153 species dispersed throughout the understory and tend to become aggregated over time.

  10. Fertility of Vriesea gigantea Gaud. (Bromeliaceae) in southern Brazil.

    PubMed

    Paggi, Gecele M; Palma-Silva, Clarisse; Silveira, Lucas C T; Kaltchuk-Santos, Eliane; Bodanese-Zanettini, Maria H; Bered, Fernanda

    2007-04-01

    Plant fertility is a central subject of many questions in plant evolutionary and conservation biology. Pollen availability, abiotic resources, and flowering pattern can limit fruit and seed production. Open pollination and pollen supplementation studies are used to estimate any pollen limitation in natural populations. To study the impact of these factors on the reproductive success of Vriesea gigantea, an epiphytic bromeliad in the Atlantic Rainforest in Brazil, its fertility in four natural populations in Itapuã State Park was assessed by considering plant and inflorescence size, flower production, fruit and seed set, flower and fruit set pattern, and seed viability and germination rate. Supplemental pollination in adult plants was used to determine whether fruit production in V. gigantea is limited by reception of pollen. The results showed that V. gigantea has a high production of flowers, fruits, and seeds. Seeds are highly viable in all populations, presenting an average germination rate of 94% (SE ± 3.5). Plants of V. gigantea from Itapuã State Park are highly fertile. The high proportion of fruit and seed set after manual hand pollination indicates that the species is self-compatible. Pollination treatments showed evidence of pollinator limitation in the Itapuã State Park population.

  11. How Will Climate Change Impact Water Consumption for Rice Irrigation in Southern Brazil?

    NASA Astrophysics Data System (ADS)

    dos Santos, T. V.; Twine, T. E.

    2015-12-01

    Globally, agricultural water use accounts for most of the water that is withdrawn from surface water and groundwater. Rice, one of the world's leading food crops, requires that fields be continuously flooded to obtain optimal yields. High air temperature and consecutive rainless days in rice-growing areas can significantly reduce rice yields, leading to food scarcity. Climate change is expected to affect water demand for rice via changes in rainfall regime, soil water balance, and evapotranspiration. Higher temperatures and increased variability of precipitation are predicted to increase water demand and could potentially require more irrigation in lowland rice-growing areas. In this study we present the first results from model simulations in which we integrated a rice model into the Agro-IBIS dynamic ecosystem model. We predict the impact of climate change on the water use requirement of rice production in southern Brazil and evaluate changes in irrigation needed to meet minimum water demand to sustain current yields. Brazil is the 9th top rice producer in the world, and southern Brazil accounts for about 80% of the national production. The Agro-IBIS model was driven with historic weather data provided by CRU (1961-90) and with two future climate scenarios from the Coupled Model Intercomparison Project Phase 5 (CMIP5) for 2015-2100 - Representative Concentration Pathways 4.5 (RCP45) and 8.5 (RCP85). On an hourly time step, Agro-IBIS accounts for gains (precipitation) and losses (evaporation, transpiration, infiltration and runoff) of water in each grid cell, and uses rules to irrigate in order to maintain a specific height of standing water on the field. Simulated historic and future amounts of irrigated water needed to maintain this water height will be evaluated to predict future water demand for rice production in southern Brazil.

  12. Leprosy in a University Hospital in Southern Brazil*

    PubMed Central

    de Lima, Adma Silva; Pinto, Karin Cristine; Bona, Míryan Priscilla Santos; de Mattos, Suelen Mayara Lopes; Hoffmann, Marina Portiolli; Mulinari-Brenner, Fabiane Andrade; Ottoboni, Vanessa Cristhine Dallolmo

    2015-01-01

    BACKGROUND Leprosy is an infectious disease that may lead to irreversible nerve damage, compromising patient's quality of life and leading to loss of working years. OBJECTIVES To evaluate the epidemiological profile of patients followed at a University Hospital. MATERIALS AND METHODS This is a retrospective observational study, based on a review of medical records. We studied the clinical and epidemiological features of patients with leprosy monitored at the Hospital de Clínicas of the Federal University of Paraná between January 2005 and January 2010. RESULTS The mean age was 47.51, while 35.94% of patients were aged 41-60. The male:female rate was 1.8:1. The most prevalent occupations were: retired, students or rural workers. Patients came mainly from Curitiba or nearby areas, but there were also patients from the countryside. The mean diagnostic delay was 24.57 months. Multibacillary forms prevailed, with the lepromatous variety being the most common, closely followed by the borderline type. Neural enlargement was found in more than 50% of the patients and 48.44% of them developed reactional states. Hemolysis was the most commonly detected drug side effect. Initial functional evaluation was possible in 70% of patients, 55% of whom had disabilities upon diagnosis. The most prevalent associated disease was hypertension. CONCLUSIONS This study showed an important diagnostic delay and a high rate of sequelae in this specific population. Brazil is one of the few remaining countries that has not yet eradicated leprosy and it is important to improve health policies in order to prevent sequelae and achieve eradication. PMID:26560210

  13. Reconnaissance for radioactive materials in the southern part of Brazil

    USGS Publications Warehouse

    Pierson, Charles T.; Haynes, Donald D.; Filho, Evaristo Ribeiro

    1957-01-01

    During 1954-1956 a reconnaissance for radioactive minerals was made with carborne, airborne and handborne scintillation equipment in the southern Brazilian states of Rio de Janeiro, Sao Paulo, Parana, Santa Catarina and Rio Grande do Sul. During the traverse covering more than 5,000 kilometers the authors checked the radioactivity of Precambrian igneous and metamorphic rocks, Paleozoic, Mesozoic and Cenozoic sedimentary rocks, and Mesozoic alkalic intrusive and basaltic extrusive rocks. The 22 samples collected contained from 0.003 to 0.029 percent equivalent uranium oxide and from 0.10 to 0.91 percent equivalent thorimn; two samples were taken from radioactive pegmati tes for mineralogic studies. None of the localities is at present a commercial source of uranium or thorium; however, additional work should be done near the alkalic stock at Lages in the State of Santa Catarina and at the Passo das Tropas fossil plant locality near Santa Maria in the state of Rio Grande do Sul. Near Lages highly altered alkalic rock from a dike contained 0.026 percent uranium oxide. At Passo das Tropas highly altered, limonite-impregnated sandstone from the Rio do Rasto group of sedimentary rocks contained 0.029 percent uranium oxide.

  14. Human and animal leptospirosis in Southern Brazil: A five-year retrospective study.

    PubMed

    Jorge, Sérgio; Schuch, Rodrigo Andrade; de Oliveira, Natasha Rodrigues; da Cunha, Carlos Eduardo Pouey; Gomes, Charles Klazer; Oliveira, Thais Larré; Rizzi, Caroline; Qadan, Aisha Farid; Pacce, Violetta Dias; Coelho Recuero, Ana Lúcia; Soares Brod, Claudiomar; Dellagostin, Odir Antônio

    Leptospirosis is an emerging zoonosis attributed to multiple reservoirs. Climatic conditions influence the transmission of pathogenic leptospires, which require warm and humid conditions for survival. The influence of seasonality in human and animal leptospirosis in the subtropical region of Brazil remains poorly understood. We performed a retrospective study to describe the patterns of human and animal exposure to leptospirosis and their association with precipitation events in Southern Brazil. Rainfall data were obtained from satellite images. Serum samples were tested using the microscopic agglutination test (MAT); samples with titer ≥ 100 were defined as seroreactive. Linear regression and Pearson's correlation were performed to assess whether there is a relationship between these variables. We found that precipitation events were not significantly associated with the exposure to leptospirosis in humans or animal species, except for dogs. The interspecies analysis revealed an association between canine and human exposure to leptospirosis. Leptospira kirschneri serovar Butembo (serogroup Autumnalis) presented the highest seroreactivity in humans. This study provides valuable insights in human and animal leptospirosis in Southern Brazil. These insights will be essential to design intervention measures directed to reduce disease dissemination. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. Managing fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), with Bt maize and insecticides in southern Brazil.

    PubMed

    Burtet, Leonardo M; Bernardi, Oderlei; Melo, Adriano A; Pes, Maiquel P; Strahl, Thiago T; Guedes, Jerson Vc

    2017-07-11

    Maize plants expressing insecticidal proteins of Bacillus thuringiensis are valuable options for managing fall armyworm (FAW), Spodoptera frugiperda, in Brazil. However, control failures were reported, and therefore insecticides have been used to control this species. Based on these, we evaluated the use of Bt maize and its integration with insecticides against FAW in southern Brazil. Early-planted Agrisure TL, Herculex, Optimum Intrasect and non-Bt maize plants were severely damaged by FAW and required up to three insecticidal sprays. In contrast, YieldGard VT Pro, YieldGard VT Pro 3, PowerCore, Agrisure Viptera and Agrisure Viptera 3 showed little damage and did not require insecticides. Late-planted Bt maize plants showed significant damage by FAW and required up to four sprays, with the exceptions of Agrisure Viptera and Agrisure Viptera 3. Exalt (first and second sprays); Lannate + Premio (first spray) and Avatar (second spray); and Karate + Match (first spray) and Ampligo (second spray) were the most effective insecticides against FAW larvae in Bt and non-Bt maize. Maize plants expressing Cry proteins exhibited FAW control failures in southern Brazil, necessitating insecticidal sprays. In contrast, Bt maize containing the Vip3Aa20 protein remained effective against FAW. However, regardless of the insecticide used against FAW surviving on Bt maize, grain yields were similar. © 2017 Society of Chemical Industry. © 2017 Society of Chemical Industry.

  16. Potentially inappropriate medications among older adults in Pelotas, Southern Brazil.

    PubMed

    Lutz, Bárbara Heather; Miranda, Vanessa Irribarem Avena; Bertoldi, Andréa Dâmaso

    2017-06-22

    To assess the use of potentially inappropriate medications among older adults. This is a population-based cross-sectional study with 1,451 older individuals aged 60 years or more in the city of Pelotas, State of Rio Grande do Sul, Brazil, in 2014. We have investigated the use of medications in the last 15 days. Using the Beers criteria (2012), we have verified the use of potentially inappropriate medications and their relationship with socioeconomic and demographic variables, polypharmacy, self-medication, and burden of disease. Among the 5,700 medications used, 5,651 could be assessed as to being inappropriate. Of these, 937 were potentially inappropriate for the older adults according to the 2012 Beers criteria (16.6%). Approximately 42.4% of the older adults studied used at least one medication considered as potentially inappropriate. The group of medications for the nervous system accounted for 48.9% of the total of the potentially inappropriate medications. In the adjusted analysis, the variables female, advanced age, white race, low educational level, polypharmacy, self-medication, and burden of disease were associated with the use of potentially inappropriate medications. It is important to known the possible consequences of the use of medication among older adults. Special attention should be given to the older adults who use polypharmacy. Specific lists should be created with more appropriate medications for the older population in the National Essential Medicine List. Avaliar o uso de medicamentos potencialmente inadequados entre idosos. Estudo transversal de base populacional com 1.451 idosos com 60 anos ou mais em Pelotas, RS, em 2014. Investigou-se o uso de medicamentos nos últimos 15 dias. Utilizando os critérios de Beers (2012), verificou-se a potencial inadequação dos medicamentos e sua relação com variáveis socioeconômicas e demográficas, polifarmácia, automedicação e carga de doença. Dentre os 5.700 medicamentos utilizados, 5

  17. Fabric evolution of polydeformed orthogneisses and quartzites along the Curitiba Shear Zone, Curitiba Domain, Southern Brazil

    NASA Astrophysics Data System (ADS)

    Cabrita, Dina; Salamuni, Eduardo; Lagoeiro, Leonardo

    2017-08-01

    In Southern Brazil there is a series of strike-slip shear zones that represent the late-collisional event of Western Gondwana in the Neoproterozoic-Eopaleozoic transition. The Curitiba Shear Zone (CSZ) is a part of this strike-slip regime separating two different domains: the supracrustal metasedimentary rocks and the basement composed of orthogneiss, migmatite and quartzite. The absence of detailed structural data for this discontinuity has motivated us to study this important part of the geology of southern of Brazil. The main purpose of this work is the characterization of meso and microstructures and determination of crystallographic fabric of quartz in orthogneisses and quartzites along the CSZ southern block. Structural data suggest that the CSZ consists of one ductile-brittle strike-slip deformation phase leading to the deformation of orthogneiss and quartzites of the Atuba Complex. The ductile regime was responsible for the formation of low-grade mylonite in greenschist facies. Crystallographic texture indicate a restricted recrystallization of quartz mainy via dislocation creep through activation ob basal slip system. The brittle regime led to the formation of cataclasites, faults and joints, with a mean orientation of N50E/75SE and N33E/80SE, respectively. Extensive dissolution-precipitation creep affected the rocks and infilled veins with carbonate and quartz.

  18. Increase of chronic low back pain prevalence in a medium-sized city of southern Brazil

    PubMed Central

    2013-01-01

    Background Chronic low back pain (CLBP) is a highly disabling morbidity with high social, economic and individual effects. Demographic, occupational and behavioral changes that took place in Brazil over the last decade are related with an increasing burden of chronic conditions. Despite these changes, comparison studies on CLBP prevalence and associated factors, over time are scarce in the literature in general, and unknown in Brazil. The present study compared the CLBP prevalence in a medium sized city in Brazil between the years 2002 and 2010 and examined factors associated with prevalence in 2010. Methods Two cross-sectional studies with similar methodology were conducted in a medium-sized city in southern Brazil, in 2002 and 2010. 3182 individuals were interviewed in the first study and 2732 in the second one, all adults aged twenty years or more. Those who reported pain for seven weeks or more in the last three months in the lumbar region where considered cases of CLBP. Results The CLBP prevalence increased from 4.2% to 9.6% in 8 years. In most of the studied subgroups the CLBP prevalence has at least doubled and the increase was even larger among younger individuals with more years of education and higher economic status. Conclusions Increase in CLBP prevalence is worrisome because it is a condition responsible for substantial social impact, besides being an important source of demand for health services. PMID:23634830

  19. Psychiatry, Sex, and Science: The Making of "Adolescent" Motherhood in Southern Brazil.

    PubMed

    Béhague, Dominique P

    2017-04-28

    Research linking teen motherhood to psychoneurodevelopmental causes and pathologies has proliferated in the past two decades. In Brazil, a psychodevelopmental project of teen motherhood has gained traction despite many experts' long-standing commitment to psychodynamic psychiatry and social epidemiology, generating epistemic tension rather than substitution. Drawing on historical ethnography conducted in Southern Brazil, I explore how this project materialized through the co-production of epistemic struggles, remedial interventions, and ontological politics. In showing how this co-production became interwoven with incremental changes in young women's emotions, sexualities, relationships, and bodies, I describe how one particular "kind" of teen motherhood emerged and became entangled with both psychiatric knowledge-production and the angst of working-class political agency. In giving women a contested psychiatric language with which to rework their social-moral worlds, I argue that science did more than conceptualize teen childbearing in pathological terms; it contributed to its troubled transformation.

  20. Occurrence of Infected Free-Living Amoebae in Cooling Towers of Southern Brazil.

    PubMed

    Soares, Scheila S; Souza, Thamires K; Berté, Francisco K; Cantarelli, Vlademir V; Rott, Marilise B

    2017-08-24

    This study determined the occurrence of potentially pathogenic free-living amoebae (FLA) and bacteria associated with amoebae in air-conditioning cooling towers in southern Brazil. Water samples were collected from 36 cooling systems from air-conditioning in the state of Rio Grande do Sul, Brazil. The organisms were identified using polymerase chain reaction (PCR) and sequencing automated. The results showed that these aquatic environments, with variable temperature, are potential "hot spots" for emerging human pathogens like free-living amoebae and bacteria associated. In total, 92% of the cooling-tower samples analyzed were positive for FLA, and Acanthamoeba was the dominant genus by culture and PCR. Amoebal isolates revealed intracellular bacteria in 39.3% of them and all were confirmed as members of the genus Pseudomonas. The results obtained show the important role of cooling towers as a source of amoebae-associated pathogens.

  1. Depredation of domestic herds by pumas based on farmer's information in Southern Brazil.

    PubMed

    Schulz, Francine; Printes, Rodrigo C; Oliveira, Larissa R

    2014-10-15

    Large carnivores such as pumas are frequently killed due to conflicts with human populations involving predation on domestic herds. In Southern Brazil, traditional pasture systems, where animals feed without specific husbandry practices is typical, becoming the herds vulnerable to puma attacks. The aim of this study was to examine the conflict between local people and pumas in a Protected Areas mosaic in southern Brazil. Forty-five face-to-face interviews with local people were performed during the year of 2011, using a structured questionnaire with open and closed questions about puma attack episodes in some farms. Based on responses, the conflict and puma attacks were described, and the characteristics of attacked farms and estimated financial losses were evaluated. The first respondents were indicated by the Local Environmental Agency, and the others were indicated by the first one and so on, which is known as "snow-ball" method. Our data suggested that pumas used to attack in unfavorable conditions of visibility (foggy days) and on easier prey (e.g. sheep). Most of the attacks reported were close to forested areas and were focused on free herds during feeding activities. Some farmers said they gave up their sheep breeding activity due to losses caused by puma attacks. However, some farmers could over estimate their losses. Moreover, pumas were considered a threat to domestic herds and respondents mentioned cases of illegal puma hunting in the area. The results of questionnaires suggested that puma attack episodes were related to fragmentation of their habitat associated to incorrect management of herds in the farms studied. The diagnosis of this type of conflict and the characterization of most attacked sites are extremely important to create strategies to prevent and control attacks by wild carnivores. Deep changes in husbandry practices added to educational programs should be implemented, in order to maintain the sustainability of rural activities as well as

  2. An early freeze in southern Brazil in April 1999 and its NWP guidance

    NASA Astrophysics Data System (ADS)

    Satyamurty, Prakki; Ferreira Bustamante Fonseca, Josiane; Bottino, Marcus Jorge; Seluchi, Marcelo E.; Maciel Lourenço, Maria Crstina; Gonçalves de Gonçalves, Luis Gustavo

    2002-03-01

    A cold air outbreak occurred in the third week of April 1999 in southern Brazil. A synoptic study of this rare event is undertaken. A surface high pressure cell in the Pacific Ocean off the coast of Chile slowly built up and moved eastward on 14 April. It started crossing the Andes, acquiring the characteristic shape of a bean on 15th, and separated into two cells later in the day. The cell on the lee side of the mountains moved gradually northwards over central South America affecting northern Argentina, Paraguay, Uruguay, Bolivia and southern Brazil. The temperature fell 15°C in the state of Mato Grosso (about 15°S). In southern Brazil temperatures below freezing were registered in some places on the 17th and 18th and frost occurred in many states. There was snowfall in the uplands of Santa Catarina on the 17th, and such an early snow had not been recorded for 30 years. Baroclinic synoptic wave intensification followed by surface low development in association with a cut-off low formation in the middle and upper troposphere were responsible for strong surface southerlies over Argentina on the 16th and 17th. Cold air advection by the southerlies in the eastern sector of the high-pressure centre was responsible for the northward projection of the high pressure cell east of the Andes. The whole event was well predicted by the Centro de Previsão de Tempo e Estudos Climáticos/Instituto Nacional de Pesquisas Espaciais (CPTEC/INPE) operational global and regional models with lead times of 120 and 60 hours respectively

  3. Multiparasitism in a wild cat (Leopardus colocolo) (Carnivora: Felidae) in southern Brazil.

    PubMed

    Gressler, Lucas Trevisan; Noll, Jéssica Caroline Gomes; Freitas, Ítallo Barros de; Monteiro, Silvia Gonzalez

    2016-01-01

    Parasitic diseases reflect the health and balance of ecosystems, affecting not only individuals but also entire populations or communities. The aim of this study was to report on the diversity of parasitic helminths detected in the feces of a wild feline in southern Brazil. Parasites were obtained from fecal samples, and four techniques were used for parasitological examination: direct examination, centrifugal flotation with zinc sulfate (Faust technique), simple sedimentation (Hoffman technique) and Baermann-Moraes. The parasites were identified through micrometry and morphology, as follows: Ancylostoma sp., Toxocara sp., Trichuridae, Aelurostrongylus abstrusus, Alaria sp., and Spirometra sp. We recorded the genus Ancylostoma parasitizing L. colocolo for the first time.

  4. A case of yellow fever in a brown howler (Alouatta fusca) in Southern Brazil.

    PubMed

    Sallis, Eliza Simone Viégas; de Barros, Vera Lúcia Reis Souza; Garmatz, Shana Letícia; Fighera, Rafael Almeida; Graça, Dominguita Lühers

    2003-11-01

    Many brown howlers (Alouatta fusca) have died in a 3-month period in a subtropical forest in Southern Brazil. One was examined after a systemic illness. According to clinical signs, and necropsy and histopathology findings, yellow fever virus (YFV) infection was suspected. Tissue sections from liver, kidney, and lymphoid organs were screened by immunohistochemistry for YFV antigens. Cells within those tissues stained positively with a polyclonal antibody against YFV antigens (1:1,600 dilution), and yellow fever was diagnosed for the first time in the brown howler in the area.

  5. Gastrointestinal parasites of owls (Strigiformes) kept in captivity in the Southern region of Brazil.

    PubMed

    da Silva, Aleksandro S; Zanette, Régis A; Lara, Valéria M; Gressler, Luciane T; Carregaro, Adriano B; Santurio, Janio M; Monteiro, Silvia G

    2009-01-01

    The aim of this research was to study the gastrointestinal parasitism in 12 adult owls kept in captivity in the Southern region of Brazil. Cloacal contents of the species Rhinoptynx clamator, Tyto alba, Athene cunicularia, Megascops spp., and Bubo virginianus were evaluated. Feces and urine were collected and analyzed by the zinc sulfate centrifugal-flotation method and stained by the modified Ziehl-Neelsen technique. Eggs of Capillaria spp. and Strongylida, oocysts of Cryptosporidium spp., Eimeria spp., and Isospora spp. were observed. The birds showed no clinical signs, probably due to the mild nature of the infection.

  6. Evidence for spawning aggregations of the endangered Atlantic goliath grouper Epinephelus itajara in southern Brazil.

    PubMed

    Bueno, L S; Bertoncini, A A; Koenig, C C; Coleman, F C; Freitas, M O; Leite, J R; De Souza, T F; Hostim-Silva, M

    2016-07-01

    In this study, seasonal numerical abundance of the critically endangered Atlantic goliath grouper Epinephelus itajara was estimated by conducting scuba dive surveys and calculating sightings-per-unit-effort (SPUE) at three sites in southern Brazil. Seasonal differences in size and reproductive condition of captured or confiscated specimens were compared. The SPUE differed significantly with season, increasing in late spring and peaking during the austral summer months. A significant effect was observed in the number of fish relative to the lunar cycle. All females sampled during the summer were spawning capable, while all those sampled during other seasons were either regressing or regenerating. What these data strongly infer is that the E. itajara spawning aggregation sites have been located in the southern state of Paraná and the northern state of Santa Catarina and summer is the most likely spawning season. Size frequency distributions, abundance and reproductive state were estimated and correlated with environmental variables.

  7. Frequency of reported European ancestry among multiple sclerosis patients from four cities in the southern and southeastern regions of Brazil.

    PubMed

    Comini-Frota, Elizabeth Regina; Brum, Doralina Guimarães; Kaimen-Maciel, Damacio Ramon; Fragoso, Yara Dadalti; Barreira, Amilton Antunes; Donadi, Eduardo Antonio

    2013-09-01

    Recent reports on the prevalence of multiple sclerosis (MS) have described discrepancies between the rates in cities in the northeastern and southeastern regions of Brazil, representing a north-south gradient. European immigrants settled in southeastern and southern Brazil at the beginning of the twentieth century. In this study, we report the frequency of European ancestors among Brazilian MS patients in four cities in the southern and southeastern regions of Brazil. A total of 652 consecutive patients with confirmed MS diagnoses seen at four centers in Belo Horizonte, Ribeirão Preto, Londrina and Santos were asked about the origin of their ancestors, going back three generations. 287 (44%) reported Italian ancestry, 211 (32%) reported that all ancestors were born in Brazil, 49 (7.5%) had Portuguese ancestry and 70 (10%) had Spanish ancestry. The patients in Belo Horizonte and Londrina reported higher proportions of Italian ancestry than the proportions estimated for the populations of their respective States. Brazil has a north-south gradient of 0.91/100,000 per degree of latitude, which is higher than the gradient for Latin America. Since the largest immigrant group that settled in southern and southeastern Brazil was from Italy, it is possible that Italian immigration was one of the factors that have contributed toward increasing the prevalence of MS in these regions. Copyright © 2013 Elsevier B.V. All rights reserved.

  8. Characterisation of complete high- and low-risk human papillomavirus genomes isolated from cervical specimens in southern Brazil.

    PubMed

    Oliveira, Gisele R de; Siqueira, Juliana D; Finger-Jardim, Fabiana; Vieira, Valdimara C; Silva, Ronald L; Gonçalves, Carla V; Soares, Esmeralda A; Martinez, Ana Maria Barral de; Soares, Marcelo A

    2017-10-01

    The classification of human papillomavirus (HPV) intratypic lineages by complete genome sequencing is a determinant in understanding biological differences in association with this disease. In this work, we have characterised complete HPV genomes from southern Brazil. Fifteen cervicovaginal Pap smear negative samples previously categorised as HPV-positive were sequenced using ultradeep sequencing, and 18 complete genomes from 13 different HPV types were assembled. Phylogenetic and genetic distance analyses were performed to classify the HPV genomes into lineages and sublineages. This is the first report describing the distribution of HPV intratype lineages of high and low oncogenic risk in asymptomatic women from southern Brazil.

  9. Characterisation of complete high- and low-risk human papillomavirus genomes isolated from cervical specimens in southern Brazil

    PubMed Central

    de Oliveira, Gisele R; Siqueira, Juliana D; Finger-Jardim, Fabiana; Vieira, Valdimara C; Silva, Ronald L; Gonçalves, Carla V; Soares, Esmeralda A; de Martinez, Ana Maria Barral; Soares, Marcelo A

    2017-01-01

    The classification of human papillomavirus (HPV) intratypic lineages by complete genome sequencing is a determinant in understanding biological differences in association with this disease. In this work, we have characterised complete HPV genomes from southern Brazil. Fifteen cervicovaginal Pap smear negative samples previously categorised as HPV-positive were sequenced using ultradeep sequencing, and 18 complete genomes from 13 different HPV types were assembled. Phylogenetic and genetic distance analyses were performed to classify the HPV genomes into lineages and sublineages. This is the first report describing the distribution of HPV intratype lineages of high and low oncogenic risk in asymptomatic women from southern Brazil. PMID:28954002

  10. Wachholz, a new exquisite dinosaur-bearing fossiliferous site from the Upper Triassic of southern Brazil

    NASA Astrophysics Data System (ADS)

    Müller, Rodrigo Temp; da Rosa, Átila Augusto Stock; Roberto da Silva, Lúcio; Aires, Alex Sandro Schiller; Pacheco, Cristian Pereira; Pavanatto, Ane Elise Branco; Dias-da-Silva, Sérgio

    2015-08-01

    The present contribution reports a new fossiliferous site from the Caturrita Formation (Upper Triassic of Paraná Basin, Southern Brazil), which yields articulate, complete, associated, and exceptionally well-preserved dinosaur specimens, together with the sole occurrence of an isolated tooth ascribed to a carnivore indetermined archosauriform. These specimens increase the sauropodomorph record from this geological unit. A preliminary evaluation of such specimens suggests that sauropodomorph dinosaurs could have been more abundant in Norian Faunas from southern Brazil, considering the increased sampling here reported. So far, about six specimens are previously recognized (disregarding Guaibasaurus) from the Caturrita Formation, and here we add four specimens, or even five if consider an additional autopodium. Also, the morphology of the isolated carnivorous-type tooth differs from other described carnivorous archosauriform teeth from the Caturrita Formation. Hence, it might represent a still undescribed taxon. Further studies of these specimens will certainly provide new data regarding biostratigraphy, phylogeny, paleoecology, and taphonomy, as this new fossiliferous locality preserves one of the most representative dinosaur records from the Norian worldwide.

  11. Estimating the Soil Thermal Conductivity in a Agricultural Crop Site in Southern Brazil

    NASA Astrophysics Data System (ADS)

    Zimmer, Tamíres; Roberti, Debora; Moreira, Virnei; Silveira, Marcos

    The thermal conductivity is higher when the heat storage is higher and the soil surface temperature is lower. The soil thermal conductivity is also dependant on the soil texture, porosity and moisture. Therefore, it varies from soil to soil and in the same soil, depending on its soil moisture. In the present work, it is shown soil thermal conductivity estimates in a agricultural crop located at the Cruz Alta city in southern Brazil. Also the dynamic of soil heat flux (G) is analyzed and the soil thermal conductivity (Ks) is estimated using experimental data form soil heat flux and soil temperature in a agricultural crop farm in a subtropical location in Southern Brazil. In this specific site, there is a crop rotation scheme along the year. The soil type is Rhodic Hapludox (FAO) or Typic Haplorthox (US Soil Taxonomy), characterized as a deep, clay soil. The experimental soil heat flux was compared with estimated soil heat flux by two forms: (1) using a known Ks from literature for this type of soil; (2) using Ks estimated using the inversion of the equation Qg=-ks* ((T2-T1)/ (Z2-Z1)), where T1 and T2 are the temperature in different layers above the soil and Z2-Z1 is the difference between the positions in temperature measurement. The general results agree with the literature for the specific agricultural crop for Ks values in the current study for the measurement period.

  12. Survey of feline leukemia virus and feline coronaviruses in captive neotropical wild felids from Southern Brazil.

    PubMed

    Guimaraes, Ana M S; Brandão, Paulo E; de Moraes, Wanderlei; Cubas, Zalmir S; Santos, Leonilda C; Villarreal, Laura Y B; Robes, Rogério R; Coelho, Fabiana M; Resende, Mauricio; Santos, Renata C F; Oliveira, Rosangela C; Yamaguti, Mauricio; Marques, Lucas M; Neto, Renata L; Buzinhani, Melissa; Marques, Regina; Messick, Joanne B; Biondo, Alexander W; Timenetsky, Jorge

    2009-06-01

    A total of 57 captive neotropical felids (one Leopardus geoffroyi, 14 Leopardus pardalis, 17 Leopardus wiedii, 22 Leopardus tigrinus, and three Puma yagouaroundi) from the Itaipu Binacional Wildlife Research Center (Refúgio Bela Vista, Southern Brazil) were anesthetized for blood collection. Feces samples were available for 44 animals, including one L. geoffroyi, eight L. pardalis, 14 L. wiedii, 20 L. tigrinus, and one P. yagouaroundi. Total DNA and RNA were extracted from blood and feces, respectively, using commercial kits. Blood DNA samples were evaluated by polymerase chain reaction (PCR) for feline leukemia virus (FeLV) proviral DNA, whereas reverse transcriptase-PCR was run on fecal samples for detection of coronavirus RNA. None of the samples were positive for coronaviruses. A male L. pardalis and a female L. tigrinus were positive for FeLV proviral DNA, and identities of PCR products were confirmed by sequencing. This is the first evidence of FeLV proviral DNA in these species in Southern Brazil.

  13. Resistance to amoxicillin, clarithromycin and ciprofloxacin of Helicobacter pylori isolated from Southern Brazil patients.

    PubMed

    Picoli, Simone Ulrich; Mazzoleni, Luiz Edmundo; Fernández, Heriberto; De Bona, Laura Renata; Neuhauss, Erli; Longo, Larisse; Prolla, João Carlos

    2014-01-01

    Helicobacter pylori is a bacteria which infects half the world population and is an important cause of gastric cancer. The eradication therapy is not always effective because resistance to antimicrobials may occur. The aim of this study was to determine the susceptibility profile of H. pylori to amoxicillin, clarithromycin and ciprofloxacin in the population of Southern Brazil. Fifty four samples of H. pylori were evaluated. The antibiotics susceptibility was determined according to the guidelines of the British Society for Antimicrobial Chemotherapy and the Comité de l'Antibiogramme de la Société Française de Microbiologie. Six (11.1%) H. pylori isolates were resistant to clarithromycin, one (1.9%) to amoxicillin and three (5.5%) to ciprofloxacin. These indices of resistance are considered satisfactory and show that all of these antibiotics can be used in the empirical therapy. The antibiotics amoxicillin and clarithromycin are still a good option for first line anti-H. pylori treatment in the population of Southern Brazil.

  14. Fruits and vegetables intake and characteristics associated among adolescents from Southern Brazil

    PubMed Central

    2012-01-01

    Background Increased body weight has been associated with an unhealthy diet, low consumption of fruits and vegetables. Our objective was to investigate whether adolescents had low intake of fruits and vegetables, and whether gender, age and education could affect the feeding patterns. Methods A population-based sample of adolescents, aged 12–19 years, were randomly selected in southern Brazil and included in this cross-sectional study. The total daily consumption of fruits, vegetables, rice and beans were investigated in standardized household interviews, using a food frequency questionnaire and questions, being categorized as five or more servings per day as the five-a-day diet. ANOVA, ANCOVA, and modified Poisson regression were used in the analysis. Results Adolescents (n = 568) were included, 49.5% boys, 14.3% had overweight and 8.8% obesity. Approximately 23% of participants consumed five daily servings of fruits and vegetables. It was observed that 36.7% of boys and 31.0% of girls consumed less than one serving of fruit per day, and 58.4% and 44.6%, respectively, consumed less than one serving of vegetables. The consumption of vegetables, fruits, and rice and beans were not independently associated with gender. Overweight was associated with higher intake of five-a-day, independently of confounding factors. Conclusions Adolescents from southern Brazil have lower frequency of consumption of five servings a day of fruits and vegetables combined. PMID:23158078

  15. RESISTANCE TO AMOXICILLIN, CLARITHROMYCIN AND CIPROFLOXACIN OF Helicobacter pylori ISOLATED FROM SOUTHERN BRAZIL PATIENTS

    PubMed Central

    Picoli, Simone Ulrich; Mazzoleni, Luiz Edmundo; Fernández, Heriberto; De Bona, Laura Renata; Neuhauss, Erli; Longo, Larisse; Prolla, João Carlos

    2014-01-01

    Introduction: Helicobacter pylori is a bacteria which infects half the world population and is an important cause of gastric cancer. The eradication therapy is not always effective because resistance to antimicrobials may occur. The aim of this study was to determine the susceptibility profile of H. pylori to amoxicillin, clarithromycin and ciprofloxacin in the population of Southern Brazil. Material and methods: Fifty four samples of H. pylori were evaluated. The antibiotics susceptibility was determined according to the guidelines of the British Society for Antimicrobial Chemotherapy and the Comité de l'Antibiogramme de la Société Française de Microbiologie. Results: Six (11.1%) H. pylori isolates were resistant to clarithromycin, one (1.9%) to amoxicillin and three (5.5%) to ciprofloxacin. These indices of resistance are considered satisfactory and show that all of these antibiotics can be used in the empirical therapy. Conclusion: The antibiotics amoxicillin and clarithromycin are still a good option for first line anti-H. pylori treatment in the population of Southern Brazil. PMID:24878996

  16. Epidemiological Profile of Patients with Cutaneous Melanoma in a Region of Southern Brazil

    PubMed Central

    Moreno, Marcelo; Schmitt, Ricardo Ludwig; Lang, Maria Gabriela; Gheno, Vanessa

    2012-01-01

    Cutaneous melanoma (CM) is responsible for 75% of deaths from malignant skin cancer. The incidence of CM in the southern region of Brazil, particularly in the western region of Santa Catarina, is possibly higher than estimated. In this study, the clinical and epidemiological profile of patients with CM treated in the western region of Santa Catarina was examined. A cross-sectional study was performed with patients diagnosed with CM from January 2002 to December 2009, from 78 counties of the western region of the state of Santa Catarina. Data were collected using a protocol adapted from the Brazilian Melanoma Group and 503 patients were evaluated. The incidence and prevalence of CM found in this region are much higher than those found elsewhere in the country. This fact is most likely due to the phenotypic characteristics of the population and the high incidence of UV radiation in this region due to its location in southern Brazil, as is the case in the countries of Oceania. PMID:22548178

  17. Salmonella serotypes, resistance patterns, and food vehicles of salmonellosis in southern Brazil between 2007 and 2012.

    PubMed

    Capalonga, Roberta; Ramos, Rosane Campanher; Both, Jane Mari Correa; Soeiro, Mara Lúcia Tiba; Longaray, Solange Mendes; Haas, Simone; Tondo, Eduardo Cesar

    2014-07-14

    Previous studies have identified Salmonella as the main causative agent of foodborne diseases in the state of Rio Grande do Sul (RS), southern Brazil, between 1997 and 2006. This study aimed to describe the Salmonella serotypes, antimicrobial patterns, and food vehicles of salmonellosis that occurred in RS between 2007 and 2012. Information about Salmonella isolates and salmonellosis outbreaks registered in the official records of the Central Laboratory of RS (FEEPS/IPB-LACEN/RS) was analyzed. Among the 163 isolates investigated, 138 (84.7%) were identified as S. Enteritidis. The second and third most frequent serovars identified were S. Schwarzengrund (5.5%) and S. Typhimurium (3.7%). Homemade mayonnaise was the food vehicle most frequently identified (17.39%), followed by pastry products (15.94%) and beef (12.32%). Antimicrobial resistance was analyzed; 12 drugs were tested. Higher percentages of resistance were observed to nitrofurantoin (94.2%) and nalidixic acid (89.1%). The resistance to these two drugs was verified in 80.43% of the isolates. Multi-resistance to three and five drugs was verified in four and two isolates, respectively. Comparing the results of the present study with results of previous reports, it was possible to conclude that S. Enteritidis and homemade mayonnaise are still the main serotype and food vehicle of salmonellosis in RS and that antimicrobial resistance has been increasing among S. Enteritidis responsible for foodborne outbreaks in southern Brazil.

  18. MRSA from Santa Catarina State, Southern Brazil: intriguing epidemiological differences compared to other Brazilian regions.

    PubMed

    Silveira, Alessandro C O; Cunha, Gabriela R; Caierão, Juliana; Cordova, Caio M de; d'Azevedo, Pedro A

    2015-01-01

    Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most frequently isolated agents in both nosocomial and community settings. It is a constant challenge for antibacterial therapy. Therefore, it becomes essential to understand the epidemiology of MRSA isolates in the institution and/or region to guide empirical therapy. The objective of this study was to evaluate the epidemiological characteristics of MRSA isolates in the state of Santa Catarina, Brazil, and determine if there is a clonal spread. We evaluated 124 clinical isolates of MRSA obtained from various anatomical sites from patients in the state of Santa Catarina in Southern Brazil. The antimicrobial susceptibility profile was evaluated by disk diffusion and minimum inhibitory concentration (MIC) was determined by Etest and broth macrodilution. SCCmec types were determined by multiplex PCR and the clonal relationship among isolates was assessed by pulsed field gel electrophoresis. Antimicrobials that have demonstrated lower rates of resistance were tetracycline (20.2%), sulfamethoxazole-trimethoprim (20.2%) and chloramphenicol (12.9%). We did not detect any resistance to glycopeptides, daptomycin, linezolid, and tigecycline. SCCmec type III was predominant (54%), followed by type II (21.8%), consistent with other Brazilian studies. Twenty-six clones were observed grouping 72 (58%) isolates and no clonal relationship was observed between our isolates and the major epidemic clones circulating in Brazil. An intriguing distinct MRSA epidemiology was observed in Santa Catarina, compared to other Brazilian regions.

  19. The African cynodont Aleodon (Cynodontia, Probainognathia) in the Triassic of southern Brazil and its biostratigraphic significance

    PubMed Central

    Kammerer, Christian F.; Melo, Tomaz P.; Paes Neto, Voltaire D.; Ribeiro, Ana Maria; Da-Rosa, Átila A. S.; Schultz, Cesar L.; Soares, Marina Bento

    2017-01-01

    In this contribution we report the first occurrence of the enigmatic African probainognathian genus Aleodon in the Middle-early Late Triassic of several localities from the state of Rio Grande do Sul in southern Brazil. Aleodon is unusual among early probainognathians in having transversely-expanded postcanine teeth, similar to those of gomphodont cynognathians. This genus was previously known from the Manda Beds of Tanzania and the upper Omingonde Formation of Namibia. The Brazilian record of this genus is based upon multiple specimens representing different ontogenetic stages, including three that were previously referred to the sectorial-toothed probainognathian Chiniquodon theotonicus. We propose a new species of Aleodon (A. cromptoni sp. nov.) based on the specimens from Brazil. Additionally, we tentatively refer one specimen from the upper Omingonde Formation of Namibia to this new taxon, strengthening biostratigraphic correlations between these strata. Inclusion of A. cromptoni in a phylogenetic analysis of eucynodonts recovers it as the sister-taxon of A. brachyrhamphus within the family Chiniquodontidae. The discovery of numerous specimens of Aleodon among the supposedly monospecific Chiniquodon samples of Brazil raises concerns about chiniquodontid alpha taxonomy, particularly given the extremely broad geographic distribution of Chiniquodon. The discovery of Brazilian Aleodon and new records of the traversodontid Luangwa supports the hypothesis that at least two subzones can be recognized in the Dinodontosaurus Assemblage Zone. PMID:28614355

  20. The African cynodont Aleodon (Cynodontia, Probainognathia) in the Triassic of southern Brazil and its biostratigraphic significance.

    PubMed

    Martinelli, Agustín G; Kammerer, Christian F; Melo, Tomaz P; Paes Neto, Voltaire D; Ribeiro, Ana Maria; Da-Rosa, Átila A S; Schultz, Cesar L; Soares, Marina Bento

    2017-01-01

    In this contribution we report the first occurrence of the enigmatic African probainognathian genus Aleodon in the Middle-early Late Triassic of several localities from the state of Rio Grande do Sul in southern Brazil. Aleodon is unusual among early probainognathians in having transversely-expanded postcanine teeth, similar to those of gomphodont cynognathians. This genus was previously known from the Manda Beds of Tanzania and the upper Omingonde Formation of Namibia. The Brazilian record of this genus is based upon multiple specimens representing different ontogenetic stages, including three that were previously referred to the sectorial-toothed probainognathian Chiniquodon theotonicus. We propose a new species of Aleodon (A. cromptoni sp. nov.) based on the specimens from Brazil. Additionally, we tentatively refer one specimen from the upper Omingonde Formation of Namibia to this new taxon, strengthening biostratigraphic correlations between these strata. Inclusion of A. cromptoni in a phylogenetic analysis of eucynodonts recovers it as the sister-taxon of A. brachyrhamphus within the family Chiniquodontidae. The discovery of numerous specimens of Aleodon among the supposedly monospecific Chiniquodon samples of Brazil raises concerns about chiniquodontid alpha taxonomy, particularly given the extremely broad geographic distribution of Chiniquodon. The discovery of Brazilian Aleodon and new records of the traversodontid Luangwa supports the hypothesis that at least two subzones can be recognized in the Dinodontosaurus Assemblage Zone.

  1. Evaluation of the impact of ENSO on precipitation extremes in southern Brazil considering the ODP phases

    NASA Astrophysics Data System (ADS)

    Firpo, M. A.; Sansigolo, C. A.

    2011-12-01

    One of the most important modes of interannual variability from ocean-atmosphere system is the El Niño/Southern Oscillation - ENSO. The Brazil southern region belongs to the Southeast of South America, where there is a strong signal of ENSO, especially over the precipitation. This phenomenon can be modulated by low frequency climate patterns, especially the dominant pattern of North Pacific, called Pacific Decadal Oscillation (PDO). Attempting to better understand these interactions, the objective of this study was to investigate the seasonal impact of ENSO events over the Southern Brazil precipitation, taking into account the PDO phases. The dataset used in this study, consist of monthly precipitation records of six well distributed stations from southern Brazil (Rio Grande do Sul state). From these series it was calculated a unique index, which was categorized in three classes, in order to obtain the extremes: very below normal precipitation (below the percentile 10), normal precipitation (between percentile 10 and 90) and very above normal precipitation (above the percentile 90). To characterize the ENSO events, it was applied the Trenberth (1997) criteria in the index proposed by Bunge and Clarke (2009), which corrects the inconsistencies between the conventional SST index for Niño 3.4 region and the Southern Oscillation Index before 1950, going beyond the incoherence for decadal scale. For PDO, it was used the index proposed by Mantua et al. (1997). Contingency tables were constructed to analyze the seasonal, simultaneous, and 3, 6, 9 and 12 months lagged relationships between ENSO events (El Niño, neutral, La Niña), and extreme precipitation anomalies (categories), also considering the PDO phases during the 1913-1999 period. Moreover, a wavelet analysis was used to check the coherency and phase among these 3 times series during the 1913-2006 period. The Contingency Tables analysis showed that, generally, there were more positive (negative) precipitation

  2. A study of Solar-Enso correlation with southern Brazil tree ring index (1955- 1991)

    NASA Astrophysics Data System (ADS)

    Rigozo, N.; Nordemann, D.; Vieira, L.; Echer, E.

    The effects of solar activity and El Niño-Southern Oscillation on tree growth in Southern Brazil were studied by correlation analysis. Trees for this study were native Araucaria (Araucaria Angustifolia)from four locations in Rio Grande do Sul State, in Southern Brazil: Canela (29o18`S, 50o51`W, 790 m asl), Nova Petropolis (29o2`S, 51o10`W, 579 m asl), Sao Francisco de Paula (29o25`S, 50o24`W, 930 m asl) and Sao Martinho da Serra (29o30`S, 53o53`W, 484 m asl). From these four sites, an average tree ring Index for this region was derived, for the period 1955-1991. Linear correlations were made on annual and 10 year running averages of this tree ring Index, of sunspot number Rz and SOI. For annual averages, the correlation coefficients were low, and the multiple regression between tree ring and SOI and Rz indicates that 20% of the variance in tree rings was explained by solar activity and ENSO variability. However, when the 10 year running averages correlations were made, the coefficient correlations were much higher. A clear anticorrelation is observed between SOI and Index (r=-0.81) whereas Rz and Index show a positive correlation (r=0.67). The multiple regression of 10 year running averages indicates that 76% of the variance in tree ring INdex was explained by solar activity and ENSO. These results indicate that the effects of solar activity and ENSO on tree rings are better seen on long timescales.

  3. Hepatitis B Virus Genotype D Isolates Circulating in Chapecó, Southern Brazil, Originate from Italy

    PubMed Central

    Gusatti, Carolina Souza; Costi, Cintia; Halon, Maria Laura; Grandi, Tarciana; Medeiros, Arlete Ferrari Rech; Silva, Cláudia Maria Dornelles; Gomes, Selma Andrade; Silva, Marcia Susana Nunes; Niel, Christian; Rossetti, Maria Lucia Rosa

    2015-01-01

    Hepatitis B virus genotype A1 (HBV/A1), of African origin, is the most prevalent genotype in Brazil, while HBV/F predominates in the other South American countries. However, HBV/D is the most common in the three states of southern Brazil, where ‘islands’ of elevated prevalence, as Chapecó and other cities, have been described. In this study, 202 HBV chronic carriers attending in 2013 the viral hepatitis ambulatory of Chapecó, were investigated. In comparison with previous studies performed in the same ambulatory, a rapid aging of the HBV infected population was observed (mean age of the newly diagnosed patients increasing from 29.9 ± 10.3 years in 1996 to 44.4 ± 13.3 years in 2013), probably due to a singular vaccination schedule at Chapecó that included not only children but also adolescents. Phylogenetic and BLAST analyses (S region) classified 91 HBV isolates into genotypes A (n = 3) and D (n = 88). The majority of HBV/D isolates were closely related to D3 sequences. To understand the reasons for the absence or near absence of genotypes A and F, and how HBV/D was introduced in the south of Brazil, HBV/D infected patients were inquired about their genealogical and geographical origins. Forty-three (52%) patients have their four grandparents of Italian origin, vs. seven (8%) who have their four grandparents of Brazilian origin. At all, 65 out of 83 (78%) patients had at least one grandparent originating from Italy. Taking into consideration the fact that Italy is one of the few countries where subgenotype D3 is predominant, the results strongly suggested that HBV/D was introduced in Brazil through Italian immigration which culminated between 1870 and 1920. PMID:26275046

  4. Hepatitis B Virus Genotype D Isolates Circulating in Chapecó, Southern Brazil, Originate from Italy.

    PubMed

    Gusatti, Carolina Souza; Costi, Cintia; Halon, Maria Laura; Grandi, Tarciana; Medeiros, Arlete Ferrari Rech; Silva, Cláudia Maria Dornelles; Gomes, Selma Andrade; Silva, Marcia Susana Nunes; Niel, Christian; Rossetti, Maria Lucia Rosa

    2015-01-01

    Hepatitis B virus genotype A1 (HBV/A1), of African origin, is the most prevalent genotype in Brazil, while HBV/F predominates in the other South American countries. However, HBV/D is the most common in the three states of southern Brazil, where 'islands' of elevated prevalence, as Chapecó and other cities, have been described. In this study, 202 HBV chronic carriers attending in 2013 the viral hepatitis ambulatory of Chapecó, were investigated. In comparison with previous studies performed in the same ambulatory, a rapid aging of the HBV infected population was observed (mean age of the newly diagnosed patients increasing from 29.9 ± 10.3 years in 1996 to 44.4 ± 13.3 years in 2013), probably due to a singular vaccination schedule at Chapecó that included not only children but also adolescents. Phylogenetic and BLAST analyses (S region) classified 91 HBV isolates into genotypes A (n = 3) and D (n = 88). The majority of HBV/D isolates were closely related to D3 sequences. To understand the reasons for the absence or near absence of genotypes A and F, and how HBV/D was introduced in the south of Brazil, HBV/D infected patients were inquired about their genealogical and geographical origins. Forty-three (52%) patients have their four grandparents of Italian origin, vs. seven (8%) who have their four grandparents of Brazilian origin. At all, 65 out of 83 (78%) patients had at least one grandparent originating from Italy. Taking into consideration the fact that Italy is one of the few countries where subgenotype D3 is predominant, the results strongly suggested that HBV/D was introduced in Brazil through Italian immigration which culminated between 1870 and 1920.

  5. Denudation History and Paleogeographic Reconstruction of the Phanerozoic of southern Mantiqueira Province, Brazil

    NASA Astrophysics Data System (ADS)

    Jelinek, A. R.; Chemale, F., Jr.

    2012-12-01

    In this work we deal with the Phanerozoic history of the Southern Mantiqueira Province and adjacent areas after the orogen-collapse of the Brasiliano orogenic mountains in southern Brazil and Uruguay, based on thermocronological data (fission track and U-Th/He on apatite) and thermal history modelling. During the Paleozoic intraplate sedimentary basins formed mainly bordering the orogenic systems, and thus, these regions have not been overprinted by younger orogenic processes. In the Mesocenozoic this region was affected by later fragmentation and dispersal due to the separation of South America and Africa. Denudation history of both margins quantified on the basis of thermal history modeling of apatite fission track thermocronology indicates that the margin of southeastern Brazil and Uruguay presented a minimum 3.5 to 4.5 Km of denudation, which included the main exposure area of the Brasiliano orogenic belts and adjacent areas. The Phanerozoic evolution of the West Gondawana is thus recorded first by the orogenetic collapses of the Brasiliano and Pan-African belts, at that time formed a single mountain system in the Cambrian-Ordovician period. Subsequentlly, formed the intraplate basins as Paraná, in southeastern Brazil, and Congo and some records of the Table Mountains Group and upper section of Karoo units, in Southwestern Africa. In Permotriassic period, the collision of the Cape Fold Belt and Sierra de la Ventana Belt at the margins of the West Gondwana supercontinent resulted an elastic deformation in the cratonic areas, where the intraplate depositional basin occurred, and also subsidence and uplift of the already established Pan-African-Brasiliano Belts. Younger denudation events, due to continental margin uplift and basin subsidence, occurred during the rifting and dispersal of the South America and Africa plates, which can be very well defined by the integration of the passive-margin sedimentation of the Pelotas and Santos basins and apatite fission

  6. Two new species of Pasipha Ogren & Kawakatsu (Platyhelminthes: Continenticola) from areas of deciduous forest in southern Brazil.

    PubMed

    Amaral, Silvana Vargas Do; Leal-Zanchet, Ana Maria

    2016-09-29

    Two new species of Geoplaninae from southern Brazil are described herein. The new species, belonging to the genus Pasipha Ogren & Kawakatsu, 1990, can be distinguished from each other and from their congeners by colour pattern and characteristics of the copulatory apparatus, especially regarding the female organs and prostatic vesicle. Both new species seem to be endemic to areas covered by deciduous forest.

  7. Multi-cereal molecular surveys suggest host preference among members of the Fusarium graminearum species complex from southern Brazil

    USDA-ARS?s Scientific Manuscript database

    This study aimed to assess the extent and distribution of members of the Fusarium graminearum species complex (FGSC) associated with cereals grown in southern Brazil between 2009 and 2012. The total collection comprised 1,127 isolates, which were divided into four collections obtained from: 1) disea...

  8. HIV/AIDS Knowledge and Health-Related Attitudes and Behaviors among Deaf and Hearing Adolescents in Southern Brazil

    ERIC Educational Resources Information Center

    Bisol, Claudia Alquati; Sperb, Tania Mara; Brewer, Toye H.; Kato, Sergio Kakuta; Shor-Posner, Gail

    2008-01-01

    HIV/AIDS knowledge and health-related attitudes and behaviors among deaf and hearing adolescents in southern Brazil are described. Forty-two deaf students attending a special nonresidential public school for the deaf and 50 hearing students attending a regular public school, ages 15-21 years, answered a computer-assisted questionnaire. (There was…

  9. Hydrology evaluation of the Soil and Water Assessment Tool considering measurement uncertainty for a small watershed in southern Brazil

    USDA-ARS?s Scientific Manuscript database

    Areas under intensive tobacco crop cultivation have impacted the water balance and have become sources of environmental contamination in Southern Brazil. Correct determination of the area’s hydrology is essential since it is the driving force of sediment and nutrient loading dynamics. The Soil and W...

  10. HIV/AIDS Knowledge and Health-Related Attitudes and Behaviors among Deaf and Hearing Adolescents in Southern Brazil

    ERIC Educational Resources Information Center

    Bisol, Claudia Alquati; Sperb, Tania Mara; Brewer, Toye H.; Kato, Sergio Kakuta; Shor-Posner, Gail

    2008-01-01

    HIV/AIDS knowledge and health-related attitudes and behaviors among deaf and hearing adolescents in southern Brazil are described. Forty-two deaf students attending a special nonresidential public school for the deaf and 50 hearing students attending a regular public school, ages 15-21 years, answered a computer-assisted questionnaire. (There was…

  11. Monitoring drought occurrences using MODIS evapotranspiration data: Direct impacts on agricultural productivity in Southern Brazil

    NASA Astrophysics Data System (ADS)

    Ruhoff, Anderson

    2014-05-01

    Evapotranspiration (ET), including water loss from plant transpiration and land evaporation, is of vital importance for understanding hydrological processes and climate dynamics and remote sensing is considered as the most important tool for estimate ET over large areas. The Moderate Resolution Imaging Spectroradiometer (MODIS) offers an interesting opportunity to evaluate ET with spatial resolution of 1 km. The MODIS global evapotranspiration algorithm (MOD16) considers both surface energy fluxes and climatic constraints on ET (water or temperature stress) to predict plant transpiration and soil evaporation based on Penman-Monteith equation. The algorithm is driven by remotely sensed and reanalysis meteorological data. In this study, MOD16 algorithm was applied to Southern Brazil to evaluate drought occurrences and its impacts over the agricultural production. Drought is a chronic potential natural disaster characterized by an extended period of time in which less water is available than expected, typically classified as meteorological, agricultural, hydrological and socioeconomic. With human-induced climate change, increases in the frequency, duration and severity of droughts are expected, leading to negative impacts in several sectors, such as agriculture, energy, transportation, urban water supply, among others. The current drought indicators are primarily based on precipitation, however only a few indicators incorporate ET and soil moisture components. ET and soil moisture play an important role in the assessment of drought severity as sensitive indicators of land drought status. To evaluate the drought occurrences in Southern Brazil from 2000 to 2012, we used the Evaporative Stress Index (ESI). The ESI, defined as 1 (one) minus the ratio of actual ET to potential ET, is one of the most important indices denoting ET and soil moisture responses to surface dryness with effects over natural ecosystems and agricultural areas. Results showed that ESI captured major

  12. Milk yield and composition from Angus and Angus-cross beef cows raised in southern Brazil.

    PubMed

    Rodrigues, P F; Menezes, L M; Azambuja, R C C; Suñé, R W; Barbosa Silveira, I D; Cardoso, F F

    2014-06-01

    This study assessed milk yield and composition of Angus and Angus-cross beef cows raised in southern Brazil. A total of 128 records were collected in 2 consecutive calving seasons from cows between 3 and 5 yr of age of 4 breed compositions: Angus (ANAN), Caracu × Angus (CRAN), Hereford × Angus (HHAN), and Nelore × Angus (NEAN). These cows were mated to Brangus (BN) or Braford (BO) bulls and managed under extensive grazing conditions in southern Brazil. Milk production of these cows was assessed by 2 procedures: indirectly by the calf weigh-suckle-weigh procedure (WD) and directly by machine milking (MM). Lactation curves were estimated using nonlinear regression and the following related traits were derived: peak yield (PY), peak week (PW), total yield at 210 d (TY210), and lactation persistence (PERS). Milk composition and calf weaning weight adjusted to 210 d (WW210) were also determined. The MM technique was considered more accurate because of lower standard errors of estimated means, greater statistical power, and greater correlation between TY210 and WW210 (0.50) compared to WD (0.36). Considering the more precise evaluation by MM, the CRAN and NEAN cows had greater TY210 (1070 and 1116 kg, respectively) and PY (8.1 and 7.8 kg, respectively) compared to ANAN and HHAN cows, which had 858 and 842 kg for TY210 and 6.6 and 6.3 kg for PY, respectively. The NEAN cows had the latest PW at 10.8 wk. Late-calving cows had 21% lower TY210 compared to cows that calved earlier. Milk composition was influenced by cow genotype, with CRAN and NEAN cows producing milk with greater fat (3.8 and 3.9%, respectively) and protein (3.2 and 3.1%, respectively) content compared to ANAN and HHAN cows. Regardless of the genotype, fat, protein, and total solids increased in concentration from beginning to end of lactation, while lactose content decreased. Crossbreeding of Angus with adapted breeds of taurine or indicine origin can be effective in increasing milk yield and nutrient

  13. Geochronological Records of Southern Ribeira Belt (Brazil): New Calibrations for Gondwana History.

    NASA Astrophysics Data System (ADS)

    Cury, L. F.; Siga Junior, O., Sr.; Harara, O.; Bahniuk, A.

    2015-12-01

    The Paranaguá terrane is mainly constituted by an arc-related granitic complex, spread in a large NE-SW elongated stripe, located in Southern Ribeira Belt, Southeastern Brazil. The country rocks of these granites occur as a disrupted folded belt composed of metassedimentary rocks and gneissic-migmatitic rocks in split basement nuclei. The southern part of the Paranaguá Terrane is characterized by high angle tectonics; on the other hand, the northern portion by low angle tectonics. The transcurrent shear zones of Southern Paranaguá Terrane, comprising a sinistral kinematic with oblique component, are marked by the coexistence of strike-slip and down-dip lineations. The thrust shear zones represent a large front collision, located in the Northern Paranaguá Terrane, with north-northwest convergence and oblique components. The transition between these two distinct tectonic styles is given by N-S faults associated with a transpressive regime, with lateral ramp characteristics. Different generations of granite intrusions took place during the magmatic arc development, with petrographic, lithogeochemical and isotopic similarities suggesting emplacement stages during a late collisional event. Older U-Pb zircon ages between 620-610 Ma indicate the presence of a relatively early magmatism associated to the first stages of subduction in southern Paranaguá Terrane. The main magmatic period is characterized by Neoproterozoic records obtained by U-Pb zircon, which give ages between 600-580 Ma. Furthermore, the metamorphic peak of the metassedimentary sequence, with U-Pb monazite age of 599 ± 5 Ma, is quite close to the main magmatic events. In addition, the U-Pb zircon records obtained in crystals rims of deformed granitoids and late leucogranitic veins show ages between 530-480 Ma. This geological context suggests a complex history for Gondwana agglutination, with late stages at Cambro-Ordovician's Buzios Orogeny.

  14. Occurrences of Eimeria spp. and gastrointestinal nematodes in dairy calves in southern Minas Gerais, Brazil.

    PubMed

    Bruhn, Fábio Raphael Pascoti; Silva Júnior, Fidelis Antônio; Carvalho, André Henrique de Oliveira; Orlando, Débora Ribeiro; Rocha, Christiane Maria Barcellos Magalhães da; Guimarães, Antônio Marcos

    2012-01-01

    The aim of this cross-sectional observational study was to determine the frequency and factors associated with infection by Eimeria spp. and gastrointestinal nematodes in 356 calves on 20 dairy farms located in southern Minas Gerais, Brazil. Ten species of Eimeria spp. were identified, of which E. bovis (37.6%) and E. zuernii (17.9%) were the most frequent. From fecal cultures, four genera of gastrointestinal nematodes were recovered, of which Cooperia spp. (74.6%) and Haemonchus (19.4%) were the most frequent. Variables relating to higher levels of technology used on dairy farms showed a significant association (p < 0.05) with higher OPG and EPG counts, and are discussed in this study.

  15. Chromosome polymorphism and complements in populations of Girardia species (Platyhelminthes, Tricladida, Paludicola) from southern Brazil.

    PubMed

    Benya, E; Leal-Zanchet, A M; Santos, W H; Hauser, J; Erdtmann, B

    2007-12-01

    The karyotypes of four species of freshwater triclads of the genus Girardia (Platyhelminthes), i.e. G. schubarti, G. tigrina, G. anderlani, and G. biapertura, from populations of different localities of the Rio Grande do Sul State, in southern Brazil, were analyzed. The karyotype of G. biapertura is presented for the first time. Three basic complements of 4, 8, and 9 chromosomes were found. Diploids, triploids, or mixoploids (2n/3n) specimens were frequently detected in these populations. The basic chromosomal complement of n=9 was verified in two different species (G. biapertura and G. anderlani), presenting a large acrocentric chromosome which is rare in the family Dugesiidae. An intra and interspecific chromosomal variability was also detected and its evolutionary implications are discussed.

  16. A hybrid zone of the genus Ctenomys: A case study in southern Brazil

    PubMed Central

    Castilho, Camila S.; Gava, Adriana; de Freitas, Thales R.O.

    2012-01-01

    We describe variation at microsatellite loci and the chromosomal polymorphisms of a hybrid population, and hybridizing populations of Ctenomys minutus (the minor tuco-tuco) from the coastal plain of Rio Grande do Sul, southern Brazil. Cytogenetic analysis and a survey of six microsatellite loci included 101 specimens of C. minutus from the parental populations (2n/AN = 42/74 and 48a/76) and their contact zone. Cytogenetic analysis recorded 26 different karyotypes exhibited by 50 individuals from the hybrid population. Of the 26 karyotypes, only 14% presented a parental-like configuration, and none had the combinations of 2n and AN expected for an F1 hybrid. The remaining karyotypes were alternative hybrid forms, with 2n varying from 42 to 46 and AN from 68 to 80. These results suggest chromosomal rearrangements are only of minor significance in the establishment of reproductive barriers for this species. PMID:23412911

  17. Factors associated with condom use in women from an urban area in southern Brazil.

    PubMed

    da Silveira, Mariângela Freitas; dos Santos, Iná Silva; Béria, Jorge Umberto; Horta, Bernardo Lessa; Tomasi, Elaine; Victora, Cesar Gomes

    2005-01-01

    This study investigated the prevalence of factors associated with condom use during last sexual intercourse in 15-49-year-old women in a probabilistic sample from the urban area of Pelotas, southern Brazil. A questionnaire administered by an interviewer, investigating socioeconomic characteristics and habits, and another (self-administered) questionnaire on sexual behavior-related variables were used. Poisson regression, following a hierarchical model, was used for data analysis. 1,543 women were included, and losses and refusals amounted to 3.5%. Prevalence of condom use in last sexual intercourse was 28.0%. Condom use was positively associated with younger age, greater schooling, non-white color, single marital status, and greater number of sex partners in the previous three months. This suggests that the group most vulnerable to STDs/AIDS due to lack of condom use is white, adult women, in stable unions, with fewer sexual partners.

  18. Phytoseiid mites (Acari) associated with yerba mate in southern Brazil, with description of a new species.

    PubMed

    Gonçalves, Dinarte; Da Silva, Guilherme Liberato; Ferla, Noeli Juarez

    2013-12-11

    Yerba mate is a plant of great socioeconomic importance in southern South America. Little has been published about the phytoseiid mite fauna of yerba mate. This paper presents information about the morphology and distribution of phytoseiid mites collected in yerba mate in the Ilópolis and Putinga counties of Brazil between 2002 and 2004. Four areas with of different forms of cultivation in every county were sampled. A list of the species recorded from that state, and a key for their identification are provided. Sixteen phytoseiid mites species were identified, belonging to 11 genera in the subfamilies Amblyseiinae (13 species) and Typhlodrominae (three species). The most abundant genus was Amblyseius with three species. Phytoscutus sexpilis Muma, 1961 and Typhloseiopsis dorsoreticulatus Lofego, Demite & Feres, 2011 are reported for the first time from Rio Grande do Sul state. This study also includes the description of a new species, Typhlodromips pallinii n. sp.

  19. Podocoryna loyola, n. sp. (Hydrozoa, Hydractiniidae): a probably introduced species on artificial substrate from southern Brazil.

    PubMed

    Haddad, Maria Angélica; Bettim, Ariane Lima; Miglietta, Maria Pia

    2014-05-20

    Podocoryna loyola, a new hydractiniid species, has been found on artificial substrates in Baía de Paranaguá, southern coast of Brazil, since April 2007. Its main morphological characteristics are: (1) polymorphic colonies with reticular stolons or encrusting hydrorhiza not covered by periderm and smooth chitinous spines; (2) newly-released medusae with eight tentacles and small interradial gonads; (3) mature medusae with eight tentacles and unbranched oral lips; gastric peduncle absent. Molecular data show that P. loyola is distinct from all other examined species of Podocoryna, and from P. hayamaensis Hirohito (1988), its sister species from Japan. As the polyps having been noted only quite recently, and in having been found only on man-made objects in port areas and estuaries, the species is most likely exotic to the region.

  20. Phenotypic and Molecular Characterization of Leptospira interrogans Isolated from Canis familiaris in Southern Brazil.

    PubMed

    Jorge, Sérgio; Monte, Leonardo G; De Oliveira, Natasha R; Collares, Thais F; Roloff, Bárbara C; Gomes, Charles K; Hartwig, Daiane D; Dellagostin, Odir A; Hartleben, Cláudia P

    2015-10-01

    Leptospirosis is a zoonotic disease caused by pathogenic spirochetes from the genus Leptospira, which includes 20 species and more than 300 serovars. Canines are important hosts of pathogenic leptospires and can transmit the pathogen to humans via infected urine. Here, we report the phenotypic and molecular characterization of Leptospira interrogans isolated from Canis familiaris in Southern Brazil. The isolated strain was characterized by variable-number tandem-repeats analysis as L. interrogans, serogroup Icterohaemorrhagiae. In addition, the isolate was recognized by antibodies from human and canine serum samples previously tested by microscopic agglutination test. Ultimately, the expression of membrane-associated antigens (LipL32 and leptospiral immunoglobulin-like proteins) from pathogenic leptospires using monoclonal antibodies was detected by indirect immunofluorescence assay. In conclusion, identification of new strains of Leptospira can help in the diagnosis and control of leptospirosis.

  1. Health assessment of the oyster Crassostrea rhizophorae on the southern coast of Bahia, northeastern Brazil.

    PubMed

    Brandão, Rosana Pinho; Boehs, Guisla; Silva, Patrícia Mirella da

    2013-01-01

    This study investigated the health of natural stocks of the oyster Crassostrea rhizophorae on the southern coast of Bahia in northeastern Brazil, during summer and winter 2010, at three localities (sampling points) in the estuaries of the Maraú (Camamu Bay) and Graciosa rivers. A total of 180 oysters (30/sampling point/season) were examined macroscopically for the presence of pathogens and anatomical changes. The specimens were subsequently fixed in Davidson solution, processed for paraffin embedding, sectioned and stained with Harris' hematoxylin and eosin. Histological analysis revealed the presence of Rickettsia-like organisms (RLOs), Ancistrocoma, Trichodina, Sphenophrya, Nematopsis, Urastoma, Bucephalus in the sporocyst phase, a nonspecific metacercaria, and a metacestode of genus Tylocephalum. The prevalence of infection was low except for parasitism by Nematopsis sp. which also caused histopathological changes. The presence of Bucephalus sp. caused parasitic castration. These two pathogens significantly affect the health of C. rhizophorae.

  2. [Integrated tobacco production: health, labor, and working conditions of tobacco farmers in Southern Brazil].

    PubMed

    Riquinho, Deise Lisboa; Hennington, Élida Azevedo

    2016-12-22

    This study aimed to analyze the tobacco farming and marketing process in an integrated system and tobacco farmers' living and working conditions in Southern Brazil. A qualitative study was conducted from December 2010 to August 2011, with 31 semi-structured interviews with tobacco farmers and key informants, besides participant observation. The principal analytical reference was the ergological perspective. The integrated system allows the tobacco industry to control the amounts paid and the tobacco's quality. Tobacco growing features high cost of inputs, farmers' indebtedness, insufficient crop insurance, and intensive use of family labor. Accident and disease risks were associated with work in tobacco farming. According to the dynamic three-pole model proposed by ergology, dealing with these problems requires confronting the workers' knowledge with technical and scientific knowledge, linked with ethical and social responsibility.

  3. Absenteeism due to mental disorders in health professionals at a hospital in southern Brazil.

    PubMed

    Santana, Leni de Lima; Sarquis, Leila Maria Mansano; Brey, Christiane; Miranda, Fernanda Moura D'Almeida; Felli, Vanda Elisa Andres

    2016-03-01

    Objective To describe the health profile of mental and behavioral disorders in health professionals at a teaching hospital in southern Brazil. Methods This was a quantitative, retrospective cross-sectional epidemiological study whose data were collected through institutional documents used to feed the Health Monitoring System for Nursing Professionals and involved all absenteeism occurred in 2011. Results We found 55 records of absenteeism due to mental and behavioral disorders, a total of 317 days absent. Nursing technicians were the most absentee, with 29.09% of the records. The intensive care unit represented the sector with the highest number of days absent, 81%, and depressive episodes were the most frequent, representing 52.72% of mental disorders. Conclusion The results showed that mental disorders in health professionals are a cause for concern and urgently need intervention.

  4. Taxonomic reports of Otobothrioidea (Eucestoda, Trypanorhyncha) from elasmobranch fishes of the southern coast off Brazil.

    PubMed

    Knoff, Marcelo; Clemente, Sérgio Carmona de São; Pinto, Roberto Magalhães; Lanfredi, Reinalda Marisa; Gomes, Delir Corrêa

    2004-02-01

    Specimens of elasmobranch fishes, captured in the states of Paraná and Santa Catarina, of the southern coast off Brazil, represented by three families, four genera, and four species, were parasitized with otobothrioid trypanorhynch cestodes: Heptranchias perlo (Bonnaterre, 1788), Squalus sp. and Carcharhinus signatus (Poey, 1868) were parasitized with Progrillotia dollfusi Carvajal & Rego,1987; Prionace glauca (Linnaeus, 1758) with Molicola horridus (Goodsir, 1841) Dollfus, 1942. Details of internal morphology and/or scolex and/or proglottids surface ultrastructure, that expanded the description of M. horridus, through observations with lightfield, and/or scanning eletronic microscopy, are provided. The known geographical distribution for the species M. horridus is enlarged. P. dollfusi is reported for the first time in elasmobranchs.

  5. Radon emissions related to the granitic Precambrian shield in southern Brazil.

    PubMed

    Fianco, Ana C B; Roisenberg, Ari; Bonotto, Daniel M

    2013-01-01

    The equivalent uranium (eU) activity concentration was analysed in selected granite samples at several sites in Porto Alegre, Southern Brazil, to obtain information on the radon ((222)Rn) generation by the aquifer rock matrices. Radon analyses of ground water and soil samples were also performed. Several samples exhibited a dissolved (222)Rn activity concentration exceeding the World Health Organization maximum limit of 100 Bq l(-1). The dissolved radon content in ground waters from the Fractured Precambrian Aquifer System exhibited a direct significant correlation with the eU in the rock matrices, which is a typical result of water-rock interactions. Variation in the soil's porosity was confirmed as an important factor for (222)Rn release, as expected, due to its gaseous nature. Thus, although the calcic-alkaline to alkaline Precambrian granitoid rocks of the study area are important reservoirs for underground resources, they can release high amounts of radon gas into the liquid phase.

  6. [Use of evaluation by primary healthcare managers: a case study in southern Brazil].

    PubMed

    Nickel, Daniela Alba; Natal, Sonia; Hartz, Zulmira Maria de Araújo; Calvo, Maria Cristina Marino

    2014-12-01

    This article reports on a meta-evaluation, focused on utilization, in a state in southern Brazil. This was a single case study with primary data collection using semi-structured interviews with health department administrators and staff. Content analysis used categories defined in the evaluation matrix: political and organizational context and implementation of evaluation. The political and organizational context revealed weaknesses in the items on experience, evaluation team, and time and space for reflection. Technical autonomy was verified in the State and in one municipality. In the implementation of evaluation, evaluative quality met the established criteria, but there was no prior definition of the uses and users of evaluation. One report referred to use for planning actions and political use. The study concluded that evaluation produced important information for stakeholders, with the political and organizational context as the principal limiting factor for use.

  7. Climate affects the structure of mixed rain forest in southern sector of Atlantic domain in Brazil

    NASA Astrophysics Data System (ADS)

    Sevegnani, Lucia; Uhlmann, Alexandre; Gasper, André Luís de; Meyer, Leila; Vibrans, Alexander Christian

    2016-11-01

    This study aimed to analyze the influence of environmental factors in determining the variation in forest structure. We obtained the data through sampling units placed regularly in a grid of 10 km × 10 km in the state of Santa Catarina, southern Brazil. The axes of Detendred Correspondence Analysis summarized the vegetation structure and we used these as response variables in ordinary least square models, and environmental variables as predictors. Moran Eigenvector Maps were used as spatial predictors, enabling the variance partitioning. The results revealed influence of climatic factors, especially thermal and rainfall in determining the vegetation structure. The physical geography (high plateaus) and positioning below the Tropic of Capricorn line are the main static elements influencing the climate and therefore the vegetation.

  8. Monitoring and assessment of outcome in cases of tuberculosis in a municipality of Southern Brazil.

    PubMed

    Lima, Lílian Moura de; Harter, Jenifer; Tomberg, Jéssica Oliveira; Vieira, Dagoberta Alves; Antunes, Muriel Lucero; Cardozo-Gonzales, Roxana Isabel

    2016-03-01

    Objectives To monitor and assess the outcome of treatment for pulmonary tuberculosis in the tuberculosis control program in a prioritized municipality in Southern Brazil. Methods a quantitative study, descriptive, documentary, using records of people with tuberculosis in treatment between 2009-2013, the collection took place between June and July 2014 in the Tuberculosis Control Program. Descriptive statistics was used. Results The average number of consultations among the 629 patients was 7.2 per patient, with a mean interval of 1.03 months between visits. The average of smears was 2.7 tests per patient during the study period. The outcome of treatment was a cure rate of 87.8%, an abandonment rate of 8.3% and 6.5% of deaths. Conclusions despite the cure rate, abandonment is still high, thus, it is necessary to explore strategies for better adherence to treatment, and the commitment of the municipal administration in articulating monitoring in primary health care.

  9. A new species of Brachycephalus (Anura: Brachycephalidae) from the Quiriri mountain range of southern Brazil.

    PubMed

    Pie, Marcio R; Ribeiro, Luiz F

    2015-01-01

    A new miniaturized toadled of the genus Brachycephalus (Anura: Brachycephalidae) is described from Serra do Quiriri in the municipality of Campo Alegre, Santa Catarina, southern Brazil. Specimens were collected from the leaf litter between from 1,263 and 1,318 m above sea level. The new species is distinguished from all its congeners by the combination of the following characters: snout-vent length 9.9-13.1 mm; skin on head and dorsum without dermal co-ossification; snout mucronate in dorsal view; dorsum rugose; general color brown, with a narrow orange vertebral stripe. The region where the new species is located is also shared with other endemic anuran species and has experienced strong anthropogenic impacts,suggesting that immediate actions should be taken to ensure their long-term preservation.

  10. A new species of Brachycephalus (Anura: Brachycephalidae) from the Quiriri mountain range of southern Brazil

    PubMed Central

    Ribeiro, Luiz F.

    2015-01-01

    A new miniaturized toadled of the genus Brachycephalus (Anura: Brachycephalidae) is described from Serra do Quiriri in the municipality of Campo Alegre, Santa Catarina, southern Brazil. Specimens were collected from the leaf litter between from 1,263 and 1,318 m above sea level. The new species is distinguished from all its congeners by the combination of the following characters: snout–vent length 9.9–13.1 mm; skin on head and dorsum without dermal co-ossification; snout mucronate in dorsal view; dorsum rugose; general color brown, with a narrow orange vertebral stripe. The region where the new species is located is also shared with other endemic anuran species and has experienced strong anthropogenic impacts,suggesting that immediate actions should be taken to ensure their long-term preservation. PMID:26339556

  11. Feeding ecology and trophic comparisons of six shark species in a coastal ecosystem off southern Brazil.

    PubMed

    Bornatowski, H; Braga, R R; Abilhoa, V; Corrêa, M F M

    2014-08-01

    The diets of six shark species, Sphyrna lewini, Sphyrna zygaena, Carcharhinus obscurus, Carcharhinus limbatus, Rhizoprionodon lalandii and Galeocerdo cuvier, were investigated in a subtropical coastal ecosystem of southern Brazil. Stomach content data were obtained to assess foraging niche segregation and ontogenetic shifts in the diets of these sharks. Five of the shark species off the Paraná coast were ichthyophagous, with the exception of S. zygaena, which was teutophagous. With the exception of G. cuvier, which had a generalist diet, the other five species displayed specialization in their feeding. Ontogenetic shifts were observed in C. obscurus and S. lewini with large individuals consuming elasmobranchs. Owing to the diet overlap between C. obscurus and S. lewini, C. obscurus and C. limbatus and R. lalandii and C. limbatus, future studies on the spatial and temporal distributions of these species are needed to understand the extent of competitive interactions.

  12. Trends in burnout syndrome and emotional factors: an assessment of anesthesiologists in Southern Brazil, 2012.

    PubMed

    Freire, Patrícia Larrosa; Trentin, Jessica Puchalski; de Avila Quevedo, Luciana

    2016-01-28

    Burnout syndrome (BOS) is the result of chronic stress at work, and is characterized by emotional exhaustion (EE), depersonalization (DP), and low professional accomplishment. Anesthesiologyis a stressful profession. The aim of this study was to assess the prevalence of burnout as well as the sociodemographic and working characteristics associated with this syndrome's emotional factors, in anesthesiologists from Southern Brazil. We assessed burnout severity, depressive symptoms, and self-esteem using the Maslach burnout inventory, the Beck depression inventory, and the Rosenberg self-esteem scale, respectively. Of the 198 anesthesiologists included in this study, 48.7% were positive for burnout, 26.9% for EE, 41.3% for DP and 32.7% for low personal accomplishment. Trends in BOS was associated with a younger age, difficulty to conciliate family and work demands, low self-esteem, and depressive symptoms.

  13. Phenology of two Ficus species in seasonal semi-deciduous forest in Southern Brazil.

    PubMed

    Bianchini, E; Emmerick, J M; Messetti, A V L; Pimenta, J A

    2015-11-01

    We analyzed the phenology of Ficus adhatodifolia Schott ex Spreng. (23 fig tree) and F. eximia Schott (12 fig tree) for 74 months in a remnant of seasonal semi-deciduous forest (23° 27'S and 51° 15'W), Southern Brazil and discussed their importance to frugivorous. Leaf drop, leaf flush, syconia production and dispersal were recorded. These phenophases occurred year-round, but seasonal peaks were recorded in both leaf phenophases for F. eximia and leaf flushing for F. adhatodifolia. Climatic variables analyzed were positively correlated with reproductive phenophases of F. adhatodifolia and negatively correlated with the vegetative phenophases of F. eximia. In despite of environmental seasonality, little seasonality in the phenology of two species was observed, especially in the reproductive phenology. Both species were important to frugivorous, but F. adhatodifolia can play a relevant role in the remnant.

  14. A new species of Cacatuocotyle (Monogenea, Dactylogyridae) parasitizing two species of Astyanax (Ostariophysi, Characidae) in southern Brazil.

    PubMed

    Gallas, Moisés; Calegaro-Marques, Cláudia; Amato, Suzana B

    2014-10-01

    A new species of Cacatuocotyle is described from the external surface of Astyanax aff. fasciatus and Astyanax jacuhiensis from Lake Guaíba, State of Rio Grande do Sul, Brazil. It differs from the other three species of the genus, recorded from Mexico and the State of Paraná, Brazil, by possessing an accessory piece with a long proximal portion, a U-shaped bar with irregular posterior margin in its midportion, and a haptor containing two circular thickenings with muscular anterior margins. Only one monogenean species, Urocleidoides astyanacis (= Characithecium costaricensis) was recorded for the host Astyanax aff. fasciatus, whereas none were known for A. jacuhiensis. This paper records a new host (A. jacuhiensis) for monogeneans in southern Brazil and extends southward with the geographical distribution of the genus Cacatuocotyle to the State of Rio Grande do Sul, Brazil.

  15. Ehrlichia sp. infection in carthorses of low-income owners, Southern Brazil.

    PubMed

    Vieira, Thállitha S; Vieira, Rafael F; Krawczak, Felipe S; Soares, Herbert S; Guimarães, Ana M; Barros-Filho, Ivan R; Marcondes, Mary; Labruna, Marcelo B; Biondo, Alexander W; Vidotto, Odilon

    2016-10-01

    Although well established in dogs, Ehrlichia sp. infection has been scarcely reported in horses. The aim was to perform a comprehensive serological and molecular survey for the detection of Ehrlichia spp. in carthorses from Southern Brazil. Blood samples from 190 carthorses from Paraná State were sampled. Horses were also tested for Borrelia burgdorferi and Anaplasma phagocytophilum. Anti-Ehrlichia sp. antibodies were detected by a commercial rapid ELISA, and immunofluorescence antibody assays (IFA) with E. chaffeensis and E. canis as crude antigens. The molecular and phylogenetic analysis of Ehrlichia sp. was based on 16S rRNA and dsb genes. A total of 52 (27.4%), 4 (2.1%), and 3 (1.6%) horses were positive for Ehrlichia spp., Anaplasma spp. and Borrelia burgdorferi, respectively, by the commercial rapid ELISA. Thirty-eight (20.0%) and 37 (19.5%) horses showed anti-E. chaffeensis and anti-E. canis antibodies by IFA, respectively. One blood sample that also showed anti-E. chaffeensis antibodies was PCR positive for the 16S rRNA and dsb genes of Ehrlichia spp., showing an identity of>98.0% to the uncultured Ehrlichia sp. previously detected in Brazilian jaguars (Panthera onca). Anti-Ehrlichia sp. antibodies and Ehrlichia DNA were detected in carthorses from Southern Brazil, which may post public health concerns due to intimate contact with low-income owners. This is the first report of a natural infection of this bacteria in horses from South America. Clinical signs and the tick vector remain unknown.

  16. Stages of change for physical activity in adults from Southern Brazil: a population-based survey

    PubMed Central

    Dumith, Samuel C; Gigante, Denise P; Domingues, Marlos R

    2007-01-01

    Background There is evidence that physical activity (PA) interventions tailored to individual's stages of change (SoC) are more effective in promote behavior change than "one-size-fits-all" interventions. However, only a few researches have investigated these stages towards PA behavior in representative samples of the population. Thus, the purpose of this study was to determine the prevalence and factors associated with the SoC for PA in a probabilistic sample of adults aged 20 years or over. Methods A population-based survey was undertaken in Pelotas, Southern Brazil, in 2005. An algorithm was applied to evaluate the SoC for PA, and PA was defined as being engaged in moderate-to-vigorous PA for at least 20 minutes on three times per week. The covariates collected in the questionnaire were: sex, age, skin color, marital status, education level, economic status, family income, smoking, body mass index (BMI) and self-reported health status. Data analyses were performed through Poisson and multinomial regression, taking the sampling design into account. Results Face-to-face interviews were applied to 3136 individuals, corresponding to a response rate of 93.5%. The prevalence across the stages was as follows: 38.3% in precontemplation, 13.0% in contemplation, 19.5% in preparation, 5.2% in action and 24.0% in maintenance. The elderly, married, smokers, and those with lower socioeconomic status were less likely to adopt, initiate and maintain regular PA. Conclusion Despite the all benefits of PA, a high proportion of adults from Southern Brazil are physically inactive and do not present intention to engage in regular PA. The profile of those who are inactive but intend to do PA resembles those who are physically active. The findings of the present study can contribute to improve health behaviors and to plan health promotion strategies aimed at increasing the level of PA in the community. PMID:17555603

  17. Assignment of serotype to Salmonella enterica isolates obtained from poultry and their environment in southern Brazil

    PubMed Central

    Pulido-Landínez, M; Sánchez-Ingunza, R; Guard, J; do Nascimento, V Pinheiro

    2013-01-01

    To assess diversity of Salmonella enterica serotypes present in poultry and their environment from southern Brazil, the Kauffmann–White–Le Minor (KWL) scheme was used to serotype a total of 155 isolates. Isolates were then re-examined with nested PCR and sequencing of the dkgB-linked intergenic sequence ribotyping (ISR) region that assesses single nucleotide polymorphisms occurring around a 5S ribosomal gene. Serotypes identified were Heidelberg (40·6%), Enteritidis (34·2%), Hadar (8·4%), Typhimurium (3·9%), Gallinarum (3·2%), Agona (1·3%), Cerro (1·3%), Livingstone (1·3%), Infantis (0·6%), Isangi (0·6%), Mbandaka (0·6%), Montevideo (0·6%) and Senftenberg (0·6%). Three unique ISRs were detected from four strains. Day old chicks yielded only S. Enteritidis, whereas S. Heidelberg was most often associated with poultry carcasses. Overall agreement between KWL and ISR was 85·2%, with disagreement possibly due to the ability of ISR to detect mixtures of serotypes in culture. Overall, ISR provided more information than did KWL about the ecology of Salm. enterica on-farm. The O-antigen group D Salm. enterica serovars such as Pullorum, Gallinarum and Enteritidis appear susceptible to overgrowth by other serotypes. Significance and Impact of the Study Single nucleotide polymorphisms found in a group of poultry-associated Salmonella isolates from southern Brazil provided evidence of mixtures of serovar group D serotypes on-farm and in single samples from birds. This finding suggests that co-infection and interserotype competition of Salmonella enterica in poultry could impact the incidence of disease in animals or humans. In addition, unique serotypes were identified on-farm that escaped characterization by antibody typing. Application of cost-efficient and highly discriminatory genomic methods for assigning serotype may alter concepts about the epidemiology of Salm. enterica on-farm and in foods. PMID:23734786

  18. Hypovitaminosis D and secondary hyperparathyroidism in resident physicians of a general hospital in southern Brazil.

    PubMed

    Premaor, M O; Paludo, P; Manica, D; Paludo, A P; Rossatto, E R; Scalco, R; Furlanetto, T W

    2008-11-01

    To evaluate the prevalence of hypovitaminosis D and secondary hyperparathyroidism in resident physicians of a general hospital in southern Brazil and identify associated factors. Cross-sectional study. Resident physicians of Hospital de Clinicas de Porto Alegre, Porto Alegre, southern Brazil. Seventythree subjects age 26.4+/-1.9. Serum PTH, 25- hydroxyvitamin D [25(OH)D], total calcium, phosphorus, magnesium, creatinine, and alkaline phosphatase were measured. In addition calcium, creatinine, and magnesium were measured in urine. Fractional excretion of calcium and magnesium were calculated. Calcium intake was estimated by a food intake questionnaire. Mean serum levels of 25(OH)D were 17.9+/-8.0 ng/ml and 57.4% presented 25(OH)D below 20 ng/ml. Secondary hyperparathyroidism, defined as serum PTH > or =48 pg/ml and normal or low serum calcium, was identified in 39.7% of the individuals. Mean serum levels of magnesium were higher (p=0.02) and the fractional excretion of calcium was lower (p<0.001) in individuals with secondary hyperparathyroidism. Serum PTH levels were positively correlated with body mass index (r=0.33 and p=0.006) and serum magnesium levels (r=0.33 and p=0.02) and negatively correlated with serum 25(OH)D levels (r=-0.33 and p=0.008), estimated calcium intake (r=-0.25 and p=0.04), and fractional excretion of calcium (r=-0.34 and p=0.009). Vitamin D deficiency and secondary hyperparathyroidism was very common in resident physicians. Therefore, measures to prevent this situation should be recommended.

  19. Prevalence of sun exposure and its associated factors in southern Brazil: a population-based study.

    PubMed

    Duquia, Rodrigo Pereira; Menezes, Ana Maria Baptista; Almeida, Hiram Larangeira de; Reichert, Felipe Fossati; Santos, Iná da Silva dos; Haack, Ricardo Lanzetta; Horta, Bernardo Lessa

    2013-01-01

    Sunlight exposure is responsible for a large number of dermatological diseases. We estimated the prevalence of sunlight exposure and its associated factors in adults from southern Brazil in a cross-sectional, population-based study. We investigated a representative sample of individuals aged ≥ 20 years (n=3,136). Sunlight exposure and its associated factors were evaluated in two distinct situations: at leisure time and at work. The time period investigated ranged from December 2004 to March 2005, comprising 120 days of the highest ultraviolet index in the urban area of the city of Pelotas, in southern Brazil. The participants were asked about sunlight exposure for at least 20 minutes between 10 A.M. and 4 P.M. The analysis was stratified by sex, and sunlight exposure was grouped into five categories. Among the 3,136 participants, prevalence of sunlight exposure at the beach was 32.8% (95% CI, 30.3 - 35.2) and 26.3% (95% CI, 24.2 28.3) among men and women, respectively. The prevalence at work was 39.8% (95% CI, 37.2 - 42.4) among men and 10.5% (95% CI, 9.1 - 12.0) among women. Age was inversely associated with sunlight exposure. Family income and achieved schooling were positively associated with sunlight exposure at leisure time and inversely associated with sunglight exposure at work. Self-reported skin color was not associated. Knowledge of any friend or relative who has been affected by skin cancer was positively associated with sunlight exposure among men at work. Despite the media campaigns on the harmful effects of excessive sunlight exposure, we found a high prevalence of sunlight exposure during a period of high ultraviolet index.

  20. Wilson's disease in southern Brazil: a 40-year follow-up study.

    PubMed

    Bem, Ricardo Schmitt de; Muzzillo, Dominique Araujo; Deguti, Marta Mitiko; Barbosa, Egberto Reis; Werneck, Lineu César; Teive, Hélio Afonso Ghizoni

    2011-01-01

    Long-term data on the clinical follow-up and the treatment effectiveness of Wilson's disease are limited because of the low disease frequency. This study evaluated a retrospective cohort of Wilson's disease patients from southern Brazil during a 40-year follow-up period. Thirty-six Wilson's disease patients, diagnosed from 1971 to 2010, were retrospectively evaluated according to their clinical presentation, epidemiological and social features, response to therapy and outcome. Examining the patients' continental origins showed that 74.5% had a European ancestor. The mean age at the initial symptom presentation was 23.3 ± 9.3 years, with a delay of 27.5 ± 41.9 months until definitive diagnosis. At presentation, hepatic symptoms were predominant (38.9%), followed by mixed symptoms (hepatic and neuropsychiatric) (30.6%) and neuropsychiatric symptoms (25%). Kayser-Fleischer rings were identified in 55.6% of patients, with a higher frequency among those patients with neuropsychiatric symptoms (77.8%). Eighteen patients developed neuropsychiatric features, most commonly cerebellar syndrome. Neuroradiological imaging abnormalities were observed in 72.2% of these patients. Chronic liver disease was detected in 68% of the patients with hepatic symptoms. 94.2% of all the patients were treated with D-penicillamine for a mean time of 129.9 ± 108.3 months. Other treatments included zinc salts, combined therapy and liver transplantation. After initiating therapy, 78.8% of the patients had a stable or improved outcome, and the overall survival rate was 90.1%. This study is the first retrospective description of a population of Wilson's disease patients of mainly European continental origin who live in southern Brazil. Wilson's disease is treatable if correctly diagnosed, and an adequate quality of life can be achieved, resulting in a long overall survival.

  1. Prevalence of sun exposure and its associated factors in southern Brazil: a population-based study*

    PubMed Central

    Duquia, Rodrigo Pereira; Menezes, Ana Maria Baptista; de Almeida, Hiram Larangeira; Reichert, Felipe Fossati; dos Santos, Iná da Silva; Haack, Ricardo Lanzetta; Horta, Bernardo Lessa

    2013-01-01

    BACKGROUND Sunlight exposure is responsible for a large number of dermatological diseases. OBJECTIVE We estimated the prevalence of sunlight exposure and its associated factors in adults from southern Brazil in a cross-sectional, population-based study. METHODS We investigated a representative sample of individuals aged ≥ 20 years (n=3,136). Sunlight exposure and its associated factors were evaluated in two distinct situations: at leisure time and at work. The time period investigated ranged from December 2004 to March 2005, comprising 120 days of the highest ultraviolet index in the urban area of the city of Pelotas, in southern Brazil. The participants were asked about sunlight exposure for at least 20 minutes between 10 A.M. and 4 P.M. The analysis was stratified by sex, and sunlight exposure was grouped into five categories. RESULTS Among the 3,136 participants, prevalence of sunlight exposure at the beach was 32.8% (95% CI, 30.3 - 35.2) and 26.3% (95% CI, 24.2 28.3) among men and women, respectively. The prevalence at work was 39.8% (95% CI, 37.2 - 42.4) among men and 10.5% (95% CI, 9.1 - 12.0) among women. Age was inversely associated with sunlight exposure. Family income and achieved schooling were positively associated with sunlight exposure at leisure time and inversely associated with sunglight exposure at work. Self-reported skin color was not associated. Knowledge of any friend or relative who has been affected by skin cancer was positively associated with sunlight exposure among men at work. CONCLUSION Despite the media campaigns on the harmful effects of excessive sunlight exposure, we found a high prevalence of sunlight exposure during a period of high ultraviolet index. PMID:24068126

  2. Pairing and reproductive success in two sympatric species of Hyalella (Crustacea, Amphipoda, Dogielinotidae) from southern Brazil

    NASA Astrophysics Data System (ADS)

    da Silva Castiglioni, Daniela; Bond-Buckup, Georgina

    2008-01-01

    This study aimed at characterizing pairing and reproductive success in relation to male and female sizes of the sympatric freshwater gammarideans Hyalella pleoacuta and H. castroi from southern Brazil. These amphipods exhibit precopulatory mate guarding, in which a male will carry a potential mate beneath its ventral surface, guarding the female for several days until it molts and lays its eggs. The specimens were collected monthly with nets, from November 2003 to July 2004 in two trout aquaculture ponds at Sítio Vale das Trutas locality, São José dos Ausentes County, southern Brazil. The precopulatory pairs and ovigerous females were identified and separated in the field. In the laboratory, they were measured (cephalothorax length in mm), using a micrometer eyepiece in a stereoscopic microscope. Pairing success was estimated from the proportion of mating males and females related to their respective non-pairing individuals by size classes. Reproductive success was estimated from egg production. The mean cephalothorax length of paired males was larger than that of the unpaired males. For females, however, body size not affect pairing success for either species, because mean cephalothorax length of paired females did not differ significantly from unpaired females. Paired and unpaired males of both species of Hyalella were larger than the females. Positive assortative mating by size was observed in both species; i.e., larger males tended to pair with larger females. Male pairing success increased sharply with size. In both species, reproductive success in males increased with body size; however, the females of intermediate size classes showed greater reproductive success. This result supports the hypothesis that loading constraints play a part in structuring size-assortative pairing in these species.

  3. Environmental lead poisoning among children in Porto Alegre state, Southern Brazil.

    PubMed

    Ferron, Mariana Maleronka; Lima, André Klafke de; Saldiva, Paulo Hilário Nascimento; Gouveia, Nelson

    2012-04-01

    To estimate the prevalence of lead poisoning in children and to identify associated factors, as well as possible local sources of contamination. A cross-sectional prevalence study conducted in 2006 with a random sample of 97 children age zero to five years from a neighborhood in Porto Alegre, Southern Brazil. Blood lead levels were measured and a questionnaire administered to collect information on sociodemographics, recycling and dwelling. A preliminary environmental evaluation was carried out with direct analysis of soil and indirect analysis of air pollution with bioindicators to identify possible sources of contamination. To analyze lead concentrations from the different collection sites, for each type of material studied, ANOVA was performed with a Brown-Forsythe adjustment for heteroscedasticity and with Dunnett's T3 procedure for multiple comparisons of unequal variances. Blood lead levels > 10.0 µg/dL was found in 16.5% of children. Recycling of waste at home, low father's education level, and increased age of children were associated with increase blood lead levels. High lead levels were found in soil, and there was little indication of lead air pollution. A high prevalence of lead poisoning was identified, and the potential sources of contamination in this community appear related to waste recylcing activities. Studies should be conducted with other populations of Brazilian children and evaluate potential sources of local and general contamination, to accurately characterize this issue in Brazil.

  4. Structural framework and Mesozoic Cenozoic evolution of Ponta Grossa Arch, Paraná Basin, southern Brazil

    NASA Astrophysics Data System (ADS)

    Strugale, Michael; Rostirolla, Sidnei Pires; Mancini, Fernando; Portela Filho, Carlos Vieira; Ferreira, Francisco José Fonseca; de Freitas, Rafael Corrêa

    2007-09-01

    The integration of structural analyses of outcrops, aerial photographs, satellite images, aeromagnetometric data, and digital terrain models can establish the structural framework and paleostress trends related to the evolution of Ponta Grossa Arch, one of the most important structures of the Paraná Basin in southern Brazil. In the study area, the central-northern region of Paraná State, Brazil, the arch crosses outcropping areas of the Pirambóia, Botucatu, and Serra Geral Formations (São Bento Group, Mesozoic). The Pirambóia and Botucatu Formations are composed of quartz sandstones and subordinated siltstones. The Serra Geral Formation comprises tholeiitic basalt lava flows and associated intrusive rocks. Descriptive and kinematic structural analyses reveal the imprint of two brittle deformation phases: D1, controlled by the activation of an extensional system of regional faults that represent a progressive deformation that generated discontinuous brittle structures and dike swarm emplacement along a NW-SE trend, and D2, which was controlled by a strike-slip (transtensional) deformation system, probably of Late Cretaceous-Tertiary age, responsible for important fault reactivation along dykes and deformation bands in sandstones.

  5. Genotyping of Giardia duodenalis from Southern Brown Howler Monkeys (Alouatta clamitans) from Brazil.

    PubMed

    Volotão, A C C; Júnior, J C Souza; Grassini, C; Peralta, J M; Fernandes, O

    2008-11-25

    Giardia duodenalis is a widespread intestinal protozoan that can infect humans and animals, both domestic and wild. Independent of host, infections present with the same symptoms. However, based on host specificity, Giardia isolates have been grouped into genotypes A to G. Parasites of assemblage A and B are known to infect humans, in addition to primates and a wide variety of mammals. In Brazil, hitherto Giardia genotypes were defined only for humans and domestic animals. To evaluate the genotypes of different Giardia present among other animals, fecal samples from 28 Southern Brown Howler Monkeys (Alouatta clamitans) kept in captivity from South Brazil were screened for G. duodenalis using parasitological methods. All of them were asymptomatic, but positive for Giardia. The genotype of the G. duodenalis circulating among these animals was ascertained by molecular typing, performed using amplification and sequencing of the beta-giardin gene. Sixteen of 28 samples were successfully amplified by PCR and sequencing of this gene s revealed that all of them were of the genotype A1. These findings suggest that A. clamitans represent a potential risk of environmental contamination of a G. duodenalis genotype that also infect humans, and therefore can be considered a potential reservoir for G. duodenalis of a genotype that can also infects humans. Therefore, these results highlight a potential public health problem due to the epidemiological and molecular evidence for anthropozoonotic transmission.

  6. Detection of Neospora sp. antibodies in cart horses from urban areas of Curitiba, Southern Brazil.

    PubMed

    Villalobos, Eliana Monteforte Cassaro; Furman, Keiko Endo; Lara, Maria do Carmo Custódio de Souza Hunold; Cunha, Elenice Maria Sequetin; Finger, Mariane Angélica; Busch, Ana Paula Brenner; de Barros Filho, Ivan Roque; Deconto, Ivan; Dornbusch, Peterson Triches; Biondo, Alexander Welker

    2012-01-01

    Neospora caninum is a protozoan parasite which affects dogs as definitive hosts and several mammalian species as intermediate hosts mainly causing abortions and central nervous system disorders. The reemerging population of cart horses for carrying recycling material in urban areas of major cities in Brazil may have an impact on disease spreading, and these animals may be used as sentinels for environmental surveillance. Thus, the present study investigated the frequency of Neospora sp. antibodies in cart horses from Curitiba and surrounding areas, Paraná State, Southern Brazil. IgG antibodies against Neospora sp. were detected using indirect fluorescence antibody test (IFAT), and titers equal to or higher than 1:50 were considered reactive. Of all samples, 14/97 (14.4%) were positive: 2/29 (6.9%) were younger than 5; 5/26 (19.2%) between 6 and 9; and 6/31 (19.4%) older than 10 years of age. One of the 11 animals with unknown age was positive (9.1%). Cart horses are likely to be more exposed to dog feces and to Neospora sp. oocyst contamination in urban settings and a lower frequency of disease in dogs may have a negative impact on horse infection risk in these areas.

  7. Molecular characterization of Mycobacterium tuberculosis isolated in the State of Parana in southern Brazil.

    PubMed

    Malaghini, Marcelo; Brockelt, Sonia Regina; Burger, Marion; Kritski, Afrânio; Thomaz-Soccol, Vanete

    2009-01-01

    Sequence IS6110 has been successfully used throughout the world for characterizing the Mycobacterium tuberculosis lineages. The aim of this study was to obtain data about circulating strains of M. tuberculosis in patients from the State of Parana in southern Brazil. Sixty-two clinical specimens obtained from sputum, bronchial aspirate, biopsy and urine from 62 patients clinically diagnosed with tuberculosis and admitted to the SUS-Brazil - The Brazilian Centralized Health Service System - were genotyped by the mixed-linker PCR DNA fingerprinting technique. The analysis demonstrated that the number of copies of the IS6110 sequence per isolates varied from four to 13 bands, with an average number of 8.5. From this, 93% of the isolates presented multiple copies. Isolates with no copies of the IS6110 element were not observed. The genetic analysis by UPGMA grouped the 62 isolates by similarity into three different groups: the first group contained two strains, the second was composed of 23, and the third, a more heterogeneous group, contained 37 isolates. Only two isolates (3.2%) formed a cluster; in other words, they presented a pattern of polymorphism with similarity above 95%. Such findings suggest that in the State of Parana, illness predominantly develops through reactivation of the latent infection as opposed to exogenous transmission. The methodology used (mixed-linker PCR DNA fingerprinting) allowed for 93.5% differentiation of the isolates tested, and proved to be a powerful tool for differentiation in the molecular genotyping of M. tuberculosis.

  8. Floristic composition, environmental variation and species distribution patterns in burned grassland in southern Brazil.

    PubMed

    Overbeck, G E; Müller, S C; Pillar, V D; Pfadenhauer, J

    2006-11-01

    In regularly burned grassland on Morro Santana, Porto Alegre, RS, Brazil, we investigated differences in the floristic composition and their relation to soil properties, aspect and distance from the forest border. In 48 plots of 0.75 m2, we identified a total of 201 species from a local species pool of approximately 450 to 500 species. Most species occurred in low frequencies, showing clumpy distribution patterns in the studied area. Multivariate analysis showed that plots close to the forest edge clearly differed from plots in the open grassland concerning composition and structure. Plots exposed to the north differed from plots on the top of the hill both in the composition of species as well as in soil variables, mainly due to shallower soil in the former. No strong relation between soil properties and variation in vegetation composition could be detected at a finer scale. The studied grassland, as all grassland vegetation in southern Brazil, is very rich in species compared to other grassland formations worldwide. However, this high biodiversity and conservational value of Campos vegetation in general has so far not been recognized properly. Disturbance is essential to maintain this open vegetation type and its species richness. Fire should be considered as a management option in the absence of grazing.

  9. Echinococcus granulosus sensu lato and Taenia hydatigena in pig in southern Brazil.

    PubMed

    Monteiro, Danieli Urach; Botton, Sônia de Avila; Tonin, Alexandre Alberto; Haag, Karen Luisa; Musskopf, Germano; Azevedo, Maria Isabel; Weiblen, Carla; Ribeiro, Tatiana Correa; de la Rue, Mário Luiz

    2015-01-01

    This study aimed to identify the parasitical etiologic agents of visceral cysts in pigs from the central/northern region of Rio Grande do Sul State, Brazil. Fifty-eight cysts were found in livers during veterinary inspection of swine slaughtered from January 2008 to 2012. Collected samples were submitted to macroscopic and molecular analyzes. Polymerase chain reaction (PCR), DNA sequencing and BLAST alignment of sequences was used to molecular characterization of the samples. By PCR 10.3% (6/58) of tested samples were positive for Echinococcus granulosus sensu lato and 56.9% (33/58) for Cysticercus tenuicollis. In this study, it was verified the occurrence of larval forms of E. granulosus sensu lato and Taenia hydatigena in pig herds from the central/northern region of Rio Grande do Sul State. The presence of both parasites is relevant due to the economic losses for the meat industry. Additionally, E. granulosus sensu lato has zoonotic importance and may be infecting pig herds in southern Brazil.

  10. Source correlation of biomarkers in a mangrove ecosystem on Santa Catarina Island in southern Brazil.

    PubMed

    Silva, Cesar A; Madureira, Luiz A S

    2012-09-01

    The relative distribution of several compounds identified in four samples of recently deposited sediments of the Itacorubi Mangrove located on the Santa Catarina Island, southern Brazil, was compared with similar data on compounds extracted from fresh leaves of three mangrove species (Avicennia schaueriana, the dominant species in the area, Rhizophora mangle and Laguncularia racemosa) and the Gramineae Spartinna alterniflora. Terpenols, previously identified in mangrove species in northern Brazil, were also found. A. schaueriana mainly contains β-amyrin (90.6 μg g(-1) of extractable organic matter); low amounts of friedelin, betulin and germanicol were detected only in the leaf extract of this species. R. mangle also contained a significant amount of β-amyrin and it was the only species where taraxerol was detected. In contrast to the leaves, sediment extracts were dominated by germanicol, α-amyrin and campesterol. Despite its chemical lability, betulin was also detected. Two homologous series of α and ω-hydroxy fatty acids were detected in the acid-alkaline fraction. In spite of being reported in the literature as components of terrigenous plants, saturated ω-hydroxy acids were not identified. Our results indicate that although triterpenols may be used as biomarkers for mangrove-derived organic matter, their relative distribution can change according to the region.

  11. Systemic Histomoniasis in a Leucistic Indian Peafowl (Pavo cristatus) from Southern Brazil.

    PubMed

    Michelazzo, Mariana de Mello Zanim; Sasse, João Pedro; de Souza, Marielen; Marutani, Victor Hugo Brunaldi; Sampaio Baptista, Ana Angelita; Garcia, João Luis; Alfieri, Amauri Alcindo; Headley, Selwyn Arlington

    2017-09-01

    The pathological and molecular findings associated with Histomonas meleagridis are described in a leucistic Indian peafowl (Pavo cristatus) from Southern Brazil. The most significant gross findings were multifocal necrotizing hepatitis and diphtheric typhlitis. Histopathologic evaluation of the liver, ceca, kidney, spleen, and small intestine revealed systemic histomoniasis (SH) associated with intralesional and intravascular accumulations of histomonad organisms consistent with H. meleagridis. PCR was used to amplify the DNA of H. meleagridis from the liver, ceca, small intestine, spleen, lungs, and kidneys. Direct sequencing and phylogenetic analyses confirmed that the isolate of the flagellated trichomonad identified from this investigation is more phylogenetically related to H. meleagridis than Tetratrichomonas gallinarum, Tritrichomonas foetus, and Dientamoeba fragilis. These results confirmed the occurrence of SH in this peafowl and add to the diagnosis of this disease in birds from Brazil. This report might represent the first complete identification of spontaneous histomoniasis in a peafowl due to pathological and molecular characteristics and one of the few documented cases of SH in non-commercial birds.

  12. Particularities of Syrah wines from different growing regions of Southern Brazil: grapevine phenology and bioactive compounds.

    PubMed

    Sartor, Saionara; Malinovski, Luciane Isabel; Caliari, Vinícius; da Silva, Aparecido Lima; Bordignon-Luiz, Marilde T

    2017-05-01

    The aim of this study was to monitor the phenological parameters of grapevine (Vitis vinifera) of Syrah variety recently introduced in the region of Marari, Água Doce, Campos Novos and São Joaquim, Santa Catarina State (SC), Brazil, and to characterize the phenolic composition and antioxidant activity of wines produced from this variety of the vintages 2010 and 2011. The adaptation of the grapevine to climate conditions of these grape growing regions of Southern Brazil for this variety has also been assessed. The climate variations occurring between the phenological cycles of grapevine of Syrah for each growing region influenced the phenological extension and heat accumulation of the grapevine. The wines showed significant levels of phenolic compounds and high antioxidant activity, while catechin, a potent antioxidant, was the main phenolic compound quantified in wines. Positive correlations between the phenolic compounds and antioxidant activity were observed. The principal component analysis confirmed the influence of the growing region and vintages on the phenolic composition of wines, indicating the importance of climate conditions. This study identified possible phenolic markers indicative of climatic influence.

  13. Amphibians of Serra Bonita, southern Bahia: a new hotpoint within Brazil's Atlantic Forest hotspot.

    PubMed

    Dias, Iuri Ribeiro; Medeiros, Tadeu Teixeira; Vila Nova, Marcos Ferreira; Solé, Mirco

    2014-01-01

    We studied the amphibian community of the Private Reserve of Natural Heritage (RPPN) Serra Bonita, an area of 20 km(2) with steep altitudinal gradients (200-950 m a.s.l.) located in the municipalities of Camacan and Pau-Brasil, southern Bahia State, Brazil. Data were obtained at 38 sampling sites (including ponds and transects within the forest and in streams), through active and visual and acoustic searches, pitfall traps, and opportunistic encounters. We recorded 80 amphibian species distributed in 15 families: Aromobatidae (1), Brachycephalidae (3), Bufonidae (4), Centrolenidae (2), Ceratophryidae (1), Craugastoridae (7), Eleutherodactylidae (2), Hemiphractidae (2), Hylidae (42), Hylodidae (1), Leptodactylidae (7), Microhylidae (3), Siphonopidae (1), Odontophrynidae (3) and Pipidae (1). Species richness was positively correlated with monthly rainfall. Near 36% of the species were found in strictly forest environments, 15% are endemic to Bahia State and 77.2% are endemic to the Atlantic Forest biome. The large species diversity of this small area, the high degree of endemism and the taxonomic and biogeographic significance turn the Serra Bonita mountain into a hotpoint for amphibians within Brazil's Atlantic Forest hotspot.

  14. Occurrence of three haplotypes of Linepithema micans (Forel) (Hymenoptera: Formicidae) in Southern Brazil.

    PubMed

    Martins, C; Nondillo, A; Martins, V G; Botton, M; Bueno, O C

    2012-02-01

    Linepithema micans (Forel) (Hymenoptera: Formicidae) is reported to occur from eastern Brazil to central Argentina in pasture or grassland, forest and second growth riparian forest, nesting under stones, rotting wood, and sandy soil. However, information on this species is poor and its ecological interactions and role as pests are unknown. Linepithema humile (Mayr), a closely related species to L. micans, known as the Argentine ant, is native to South America, and was accidentally introduced to several regions of the world. Recent studies have shown that other related species, such as L. micans, could become as pestiferous as L. humile because of its phylogenetic proximity. Samples of L. micans from different habitats in Southern Brazil were characterized by sequencing of the mitochondrial DNA. Sequences were compared to previously obtained sequences from samples of L. humile and the genetic distance and differences in the tRNALeu structure were investigated. Our data identified three haplotypes of L. micans, two of which were observed in ant populations closely associated with the Brazilian ground pearl Eurhizococcus brasiliensis (Hempel) (Hemiptera: Margarodidae), a soil scale that is a serious pest of vineyards. The third haplotype was identified in ants from populations invading residences in urban habitats.

  15. Pragmatic evaluation of odour emissions from a rendering plant in southern Brazil.

    PubMed

    Vieira, Magnun M; Schirmer, Waldir N; de Melo Lisboa, Henrique; Belli Filho, Paulo; Guillot, Jean-Michel

    2016-12-01

    In the food industry and linked activities, environmental impacts relate mainly to the generation of liquid and gaseous effluents. Rendering plants, which process animal by-products, are strongly associated with malodorous emissions. Thus, effective odour-control technologies are required to minimise odour annoyance in nearby communities and thereby public complaints. In this paper, the effectiveness of a biofilter for the treatment of odours from a meat-rendering plant located in southern Brazil was evaluated based on German guideline VDI 3477:2004-11. Samples were collected upstream and downstream of the gas treatment system using a pragmatic approach in order to minimise the cost of such a study. Odour concentration was determined according to European standard EN 13725:2003. The results showed an OER of 8.82 × 10(8) ouE h(-1) and odour removal efficiency lower than the established benchmark set by SEMA Resolution 054/2006, taken as reference for being the only Brazilian regulation to establish quantitative odour emission criteria. Enhancement of the biofiltration system and/or association with other odour abatement technologies are required to increase odour removal, limit impacts and comply with regulatory requirements. The paper also includes a discussion on the current odour regulation in Brazil.

  16. High prevalence of anemia in children and adult women in an urban population in southern Brazil.

    PubMed

    Silla, Lucia Mariano da Rocha; Zelmanowicz, Alice; Mito, Ingrid; Michalowski, Mariana; Hellwing, Tania; Shilling, Marco Antonio; Friedrisch, João Ricardo; Bittar, Christina M; Albrecht, Cristina Arthmar Mentz; Scapinello, Elaine; Conti, Claudia; Albrecht, Marcia Arthmar Mentz; Baggio, Letícia; Pezzi, Annelise; Amorin, Bruna; Valim, Vanessa; Fogliatto, Laura; Paz, Alessandra; Astigarraga, Claudia; Bittencourt, Rosane Isabel; Fischer, Gustavo; Daudt, Liane

    2013-01-01

    This population-based study was designed to detect the prevalence of anemia in a healthy population of children (18 months to 7 years) and women (14 to 30 years) tested in 2006-2007 in the state of Rio Grande do Sul, Brazil as part of an effort to tackle this massive problem that still affects so many people in the XXI century. Anemia was defined according to the WHO. Capillary blood was measured and socioeconomic status was determined according to the Brazilian Association of Market Research Agencies. The median prevalence of anemia in 2198 children was 45.4% and in 1999 women 36.4%. Anemia decreased with age during childhood; although significantly more prevalent in lower classes individuals, it was also high in the upper classes. There are indirect evidences that the lack of iron supplementation and/or iron fortified food may play a role in it. Professionals and society wise measures of education have to be implemented in order to address possible biologic factors involved in childhood psychosocial development in southern Brazil.

  17. High Prevalence of Anemia in Children and Adult Women in an Urban Population in Southern Brazil

    PubMed Central

    Silla, Lucia Mariano da Rocha; Zelmanowicz, Alice; Mito, Ingrid; Michalowski, Mariana; Hellwing, Tania; Shilling, Marco Antonio; Friedrisch, João Ricardo; Bittar, Christina M.; Albrecht, Cristina Arthmar Mentz; Scapinello, Elaine; Conti, Claudia; Albrecht, Marcia Arthmar Mentz; Baggio, Letícia; Pezzi, Annelise; Amorin, Bruna; Valim, Vanessa; Fogliatto, Laura; Paz, Alessandra; Astigarraga, Claudia; Bittencourt, Rosane Isabel; Fischer, Gustavo; Daudt, Liane

    2013-01-01

    This population-based study was designed to detect the prevalence of anemia in a healthy population of children (18 months to 7 years) and women (14 to 30 years) tested in 2006–2007 in the state of Rio Grande do Sul, Brazil as part of an effort to tackle this massive problem that still affects so many people in the XXI century. Anemia was defined according to the WHO. Capillary blood was measured and socioeconomic status was determined according to the Brazilian Association of Market Research Agencies. The median prevalence of anemia in 2198 children was 45.4% and in 1999 women 36.4%. Anemia decreased with age during childhood; although significantly more prevalent in lower classes individuals, it was also high in the upper classes. There are indirect evidences that the lack of iron supplementation and/or iron fortified food may play a role in it. Professionals and society wise measures of education have to be implemented in order to address possible biologic factors involved in childhood psychosocial development in southern Brazil. PMID:23922664

  18. Geological context of the dinosauriform-bearing outcrops from the Triassic of Southern Brazil

    NASA Astrophysics Data System (ADS)

    Da-Rosa, Átila A. S.

    2015-08-01

    The Triassic of the western Gondwana (southern Brazil and northwestern Argentina) records the oldest dinosaurs. The Southern Brazilian Triassic fauna is subdivided into four assemblage zones (AZ's), recorded in alluvial (channel and floodplain) deposits, split into three third-order sequences that comprise the Santa Maria Supersequence. These deposits record dinosauriforms in three of these AZ's, mostly in near-channel environments (channel deposits, crevasse splays, distal floodplains) with different types of preservation (mostly partly articulated, with little carbonate deposition). There is faciological homogeneity within the Dinodontosaurus, Santacruzodon and Hyperodapedon AZ's, whereas change in fluvial style is recorded at the Riograndia AZ. So, further stratigraphic studies must include the recognition of post-depositional tectonism, in order to better understand the autogenic and allogenic mechanisms of deposition. It is suggested here that there is a lateral change on main channel deposition, with the areal restriction of the Santacruzodon AZ probably linked to reactivation on the Passo do Sobrado lineament and modification of the basin floor.

  19. Apple production and quality when cultivated under anti-hail cover in Southern Brazil.

    PubMed

    Bosco, Leosane Cristina; Bergamaschi, Homero; Cardoso, Loana Silveira; de Paula, Viviane Aires; Marodin, Gilmar Arduino Bettio; Nachtigall, Gilmar Ribeiro

    2015-07-01

    Anti-hail nets may change the microclimate of orchards and hence modify the physicochemical and sensory characteristics of fruits. The present study aimed to evaluate the effects of anti-hail nets on the physical, chemical, and sensory attributes of apples grown in southern Brazil. The study was conducted in commercial orchards, with apples grown under a black anti-hail net under an open sky during the 2008/2009, 2009/2010, and 2010/2011 cycles. Measurements of photosynthetically active radiation were collected at both sites. Physical, chemical, and sensory analyses of fruits were performed in the laboratory. The anti-hail net reduced incident photosynthetically active radiation by 32%. The light spectrum in the canopy changed the corresponding R/FR (red/far-red) ratio in the lower and upper canopy layers from 0.27 to 1.55, respectively. In contrast to the majority of microclimate studies carried out in the temperate zones of the northern hemisphere, this study in the southern hemisphere showed that although it reduced the incident solar radiation, the cover did not change the color or organoleptic characteristics of "Royal Gala" and "Fuji Suprema" apples. The net cover prolonged the subperiod between fruit setting and harvesting, thus slowing fruit ripening. Therefore, the use of anti-hail nets on apple orchards is a suitable alternative for the protection of apple trees against hail because it causes only small changes in the microclimate and in the maturation period, ensuring fruit production without affecting its quality.

  20. Genetic diversity of the Neotropical otter (Lontra longicaudis Olfers, 1818) in Southern and Southeastern Brazil.

    PubMed

    Trinca, C S; Waldemarin, H F; Eizirik, E

    2007-12-01

    The Neotropical otter is one of the least known otter species, and it is considered to be threatened to various degrees throughout its geographic range. Little information exists on the ecological characteristics of this species, and no genetic study has been published about it until now, hampering the design of adequate conservation strategies for its populations. To contribute with genetic information to comprehensive conservation efforts on behalf of L. longicaudis, we characterized the molecular diversity of the 5 portion of the mtDNA control region in samples from this species collected in Southern and Southeastern Brazil. The sequence analysis revealed a high level of haplotype diversity (h=0.819; SE=0.0052) and nucleotide variability ranging from 0.0039 to 0.0067. One of the sampled haplotypes was the most common in both regions and, from this sequence, several other (locally occurring) haplotypes could be derived by single point mutations. No significant genetic differentiation was observed between the Southern and Southeastern regions.

  1. Apple production and quality when cultivated under anti-hail cover in Southern Brazil

    NASA Astrophysics Data System (ADS)

    Bosco, Leosane Cristina; Bergamaschi, Homero; Cardoso, Loana Silveira; de Paula, Viviane Aires; Marodin, Gilmar Arduino Bettio; Nachtigall, Gilmar Ribeiro

    2015-07-01

    Anti-hail nets may change the microclimate of orchards and hence modify the physicochemical and sensory characteristics of fruits. The present study aimed to evaluate the effects of anti-hail nets on the physical, chemical, and sensory attributes of apples grown in southern Brazil. The study was conducted in commercial orchards, with apples grown under a black anti-hail net under an open sky during the 2008/2009, 2009/2010, and 2010/2011 cycles. Measurements of photosynthetically active radiation were collected at both sites. Physical, chemical, and sensory analyses of fruits were performed in the laboratory. The anti-hail net reduced incident photosynthetically active radiation by 32 %. The light spectrum in the canopy changed the corresponding R/FR (red/far-red) ratio in the lower and upper canopy layers from 0.27 to 1.55, respectively. In contrast to the majority of microclimate studies carried out in the temperate zones of the northern hemisphere, this study in the southern hemisphere showed that although it reduced the incident solar radiation, the cover did not change the color or organoleptic characteristics of "Royal Gala" and "Fuji Suprema" apples. The net cover prolonged the subperiod between fruit setting and harvesting, thus slowing fruit ripening. Therefore, the use of anti-hail nets on apple orchards is a suitable alternative for the protection of apple trees against hail because it causes only small changes in the microclimate and in the maturation period, ensuring fruit production without affecting its quality.

  2. HLA-F polymorphisms in a Euro-Brazilian population from Southern Brazil.

    PubMed

    Manvailer, L F S; Wowk, P F; Mattar, S B; da Siva, J S; da Graça Bicalho, M; Roxo, V M M S

    2014-12-01

    HLA-F is a non-classical major histocompatibility complex (MHC) gene. It codes class Ib MHC molecules with restricted distribution and less nucleotide variations than MHC class Ia genes. Of the 22 alleles registered on the IMGT database only four alleles encode for proteins that differ in their primary structure. To estimate genotype and allele frequencies, this study targeted on known protein coding regions of the HLA-F gene. Genotyping was performed by Sequence Base Typing (SBT). The sample was composed by 199-unrelated bone marrow donors from the Brazilian Bone Marrow Donor Registry (REDOME), Euro-Brazilians, from Southern Brazil. About 1673 bp were analyzed. The most frequent allele was HLA-F*01:01 (87.19%), followed by HLA-F*01:03 (12.31%), HLA-F*01:02 (0.25%) and HLA-F*01:04 (0.25%). Significant linkage disequilibrium (LD) was verified between HLA-F and HLA classes I and II alleles. This is the first study regarding HLA-F polymorphisms in a Euro-Brazilian population contributing to the Southern Brazilian genetic characterization.

  3. Mechanisms of northeastern Brazil rainfall anomalies due to Southern Tropical Atlantic variability

    NASA Astrophysics Data System (ADS)

    Neelin, J.; Su, H.

    2004-05-01

    Observational studies have shown that the rainfall anomalies in eastern equatorial South America, including Nordeste Brazil, have a positive correlation with tropical southern Atlantic sea surface temperature (SST) anomalies. Such relationships are reproduced in model simulations with the quasi-equilibrium tropical circulation model (QTCM), which includes a simple land model. A suite of model ensemble experiments is analysed using observed SST over the tropical oceans, the tropical Atlantic and the tropical southern Atlantic (30S-0), respectively (with climatological SST in the remainder of the oceans). Warm tropical south Atlantic SST anomalies yield positive precipitation anomalies over the Nordeste and the southern edge of the Atlantic marine intertropical convergence zone (ITCZ). Mechanisms associated with moisture variations are responsible for the land precipitation changes. Increases in moisture over the Atlantic cause positive anomalies in moisture advection, spreading increased moisture downwind. Where the basic state is far from the convective stability threshold, moisture changes have little effect, but the margins of the climatological convection zone are affected. The increased moisture supply due to advection is enhanced by increases in low-level convergence required by moist static energy balances. The moisture convergence term is several times larger, but experiments altering the moisture advection confirm that the feedback is initiated by wind acting on moisture gradient. This mechanism has several features in common with the recently published "upped-ante" mechanism for El Nino impacts on this region. In that case, the moisture gradient is initiated by warm free tropospheric temperature anomalies increasing the typical value of low-level moisture required to sustain convection in the convection zones. Both mechanisms suggest the usefulness of coordinating ocean and land in situ observations of boundary layer moisture.

  4. First occurrence of blaOXA-58 in Acinetobacter baumannii isolated from a clinical sample in Southern Brazil

    PubMed Central

    de Souza Gusatti, Carolina; Bertholdo, Lauren Martins; Otton, Letícia Muner; Marchetti, Desirée Padilha; Ferreira, Alessandra Einsfeld; Corção, Gertrudes

    2012-01-01

    This is the first report of an Acinetobacter baumannii from clinical origin carrying the blaOXA-58 gene in Brazil. The isolate included in this study was from a patient during an outbreak in Porto Alegre, RS, Southern Brazil, in 2007. It was resistant to most of the beta-lactams tested, it has also the blaOXA-65 gene and the ISAbal sequence located upstream to both blaOXA genes detected and it has a MIC of imipenem of 64 μg/mL. PMID:24031824

  5. The environmental impact on air quality and exposure to carbon monoxide from charcoal production in southern Brazil.

    PubMed

    Gomes, Gabriel Meneghetti Faé; Encarnação, Fábio

    2012-07-01

    Black wattle silviculture is an important activity in southern Brazil. Much of the wood is used in the production of charcoal and the pyrolysis products impacts on air quality. This paper estimates the level of atmospheric contamination from the production of charcoal in one region of Brazil. We describe a low-cost charcoal kiln that can capture condensable gases and we estimate the levels of exposure of kiln workers to carbon monoxide. The latter results indicated that exposure to carbon monoxide can be reduced from an average of 950 ppm to 907 ppm and the mass of gases reduced by 16.8%. Copyright © 2012 Elsevier Inc. All rights reserved.

  6. Post-collisional subvolcanic rhyolites associated with the Neoproterozoic Pelotas Batholith, southern Brazil

    NASA Astrophysics Data System (ADS)

    Oliveira, Diego Skieresz de; Sommer, Carlos Augusto; Philipp, Ruy Paulo; Lima, Evandro Fernandes de; Basei, Miguel Ângelo Stipp

    2015-11-01

    Neoproterozoic volcanic and subvolcanic rhyolitic systems in southernmost Brazil are correlated with acid magmatism linked to different petrotectonic associations of the Sul-Rio-Grandense Shield. A portion of this volcanism in the Dom Feliciano Belt is associated with the Pelotas Batholith, which resulted from magmatic episodes associated with the Ediacaran post-collisional evolution of southern Brazil. Ana Dias Rhyolite is the main subvolcanic occurrence of this volcanism that took place in the Quitéria region, in the central part of Rio Grande do Sul State. The acid magmatism has been commonly associated with the most differentiated granite suite phases during the final stages of emplacement of the Pelotas Batholith. The Ana Dias Rhyolite is characterized as an intrusive body with rocks that present a porphyritic to seriated texture and a gradational variation to fine-grained equigranular rocks. New zircon U-Pb dating indicates crystallization age of 581.9 ± 1.9 Ma for the Ana Dias Rhyolite. Geochemistry data characterize the rhyolites as belonging to the alkaline series; they present a metaluminous to peraluminous character; elevated SiO2 and alkali concentrations, high FeOt/FeOt + MgO ratios and agpaitic index; and low Al2O3, CaO, and MgO contents. The Zr, Rb, Y, Nb, and Ga concentrations are moderate when compared with the relatively low Ba and Sr contents. These geochemistry characteristics are common in acid magmas with alkaline affinity. The behavior of certain trace elements and REE demonstrate enrichment in more incompatible elements, in addition to the negative anomaly of Ba, the slight enrichment in Ce relative to adjacent elements, as well as the enrichment in K2O and Rb relative to Nb, suggesting magmas derived from mantle sources enriched in incompatible elements with some crustal contamination. The chemical characteristics are similar to those of A-type granites associated with Neoproterozoic post-collision magmatism in the Sul

  7. Changes in the Behavior of Heavy Rainfall in the Southern Brazil

    NASA Astrophysics Data System (ADS)

    Basso, Raviel; Allasia, Daniel; Tassi, Rutineia

    2017-04-01

    Heavy rainfalls are associated with several economic and environmental damages mainly in urbanized areas. Their analisys depends on the availability of a dense rainfall station's network that is absent or inaccessible in Brazil, especially for sub-daily information. This study compares the Intensity-Duration-Frequency (IDF) data presented by Pfafstetter (1957) and later reanalyzed by Torrico (1974), against the most recent IDF information in Southern Brazil (comprising the States of Rio Grande do Sul, Santa Catarina and Paraná). This IDFs's collection was obtained from many sources ranging from national and local symposia, municipalities publications manuals to books, resulting in a database of more than a hundred of IDFs equations. The rainfall heights with several durations (1h, 4h, 12h, and 24h) obtained from older (until 1955's) and newer (after 1970's) IDFs were interpolated by ordinary kriging using GIS tools. The interpolated rainfall from these different periods was compared side-by-side allowing the determination of the percentual change between them. With the exception of Florianópolis region (NE of the Santa Catarina State), the newer IDFs showed higher precipitations than observed in pre-1955's data. This indicates an increase of heavy rainfall in practically the whole area, with some exceptions in the South and Northern coastal regions, in agreement with some climate change forecast models. It was also observed a more pronounced increase of sub-daily rainfall. For example, in some places, the newer data show that almost 70% of the amount of 24 hours rainfall occurs in just one hour of rainfall, against less than 40% observed in the data from the first half of the 20th century. This result alerts not only for the necessity of storwater drainage design's review but, especially, for the establishment of standardized heavy rainfall information procedures taking into account the observed time series trend.

  8. Factors associated with caries: a survey of students from southern Brazil

    PubMed Central

    Borges, Tássia Silvana; Schwanke, Natalí Lippert; Reuter, Cézane Priscila; Neto, Léo Kraether; Burgos, Miria Suzana

    2016-01-01

    Abstract Objective: To describe the factors associated with dental caries among students from Santa Cruz do Sul, Rio Grande do Sul, Brazil. Methods: A cross-sectional study was conducted in a random sample of 623 students of both genders, aged 10-17 years old. Tooth decay was performed using the index of the World Health Organization (1997), DMFT (permanent dentition) that expresses the sum of decayed, missing and filled teeth per person. The maternal educational level was rated using criteria of the Brazilian Association of Market Research Companies. The remaining variables were obtained by a structured questionnaire. Poisson regression analysis was used to test the association between variables using robust models and a subsequently adjusted model. Data were expressed as prevalence ratio (PR). Results: Multivariate analysis identified the following factors related to the experience of dental caries: residence in rural municipalities (PR: 1.15; 95%CI: 1.0-1.3), attending a city school (PR: 3.30; 95%CI: 1.1-9.4) or a state school (PR: 3.40; 95%CI: 1.1-9.6); and having an illiterate mother or a mother that only attended up to the 4th year of school (PR: 1.67; 95%CI: 1.1-2.4) or high school (PR: 1.54; 95%CI: 1.1-2.2). Conclusions: The presence of caries in students in southern Brazil was associated with residence in rural areas, mother with little education and attendance to a public school. PMID:27477791

  9. Modeling sugarcane ripening as a function of accumulated rainfall in Southern Brazil

    NASA Astrophysics Data System (ADS)

    Cardozo, Nilceu P.; Sentelhas, Paulo C.; Panosso, Alan R.; Palhares, Antonio L.; Ide, Bernardo Y.

    2015-12-01

    The effect of weather variables on sugarcane ripening is a process still not completely understood, despite its huge impact on the quality of raw material for the sugar energy industry. The aim of the present study was to evaluate the influence of weather variables on sugarcane ripening in southern Brazil, propose empirical models for estimating total recoverable sugar (TRS) content, and evaluate the performance of these models with experimental and commercial independent data from different regions. A field experiment was carried out in Piracicaba, in the state of São Paulo, Brazil, considering eight sugarcane cultivars planted monthly, from March to October 2002. In 2003, at the harvest, 12 months later, samples were collected to evaluate TRS (kg t-1). TRS and weather variables (air temperature, solar radiation, relative humidity, and rainfall) were analyzed using descriptive and multivariate statistical analysis to understand their interactions. From these correlations, variables were selected to generate empirical models for estimating TRS, according to the cultivar groups and their ripening characteristics (early, mid, and late). These models were evaluated by residual analysis and regression analysis with independent experimental data from two other locations in the same years and with independent commercial data from six different locations from 2005 to 2010. The best performances were found with exponential models which considered cumulative rainfall during the 120 days before harvest as an independent variable ( R 2 adj ranging from 0.92 to 0.95). Independent evaluations revealed that our models were capable of estimating TRS with reasonable to high precision ( R 2 adj ranging from 0.66 to 0.99) and accuracy ( D index ranging from 0.90 to 0.99), and with low mean absolute percentage errors (MAPE ≤ 5 %), even in regions with different climatic conditions.

  10. Investigation of rainwater contamination sources in the southern part of Brazil.

    PubMed

    Hoinaski, Leonardo; Franco, Davide; Haas, Reinaldo; Martins, Renata Fátma; Lisboa, Henrique de Melo

    2014-01-01

    Rainwater quality is influenced by air pollutants and can affect sensitive ecosystems. This study was conducted to identify the sources of rainwater contamination in a receptor investigated in the southern part of Brazil. A total of 22 rainwater samples were collected at Florianópolis, Brazil. The sampling station is influenced by continental emissions (soil resuspension, traffic emissions and combustion) and marine aerosols. Over the sampling period, the average pH and electrical conductivity (EC) of the precipitation was found to be 4.97 +/- 0.41 and 14.68 microS cm(-1) +/- 13.47, respectively. In addition topH and EC, ions and trace metals in the collected rainwater were quantified. The results were investigated by a combination of techniques including principal component analysis (PCA), a back trajectory model and other statistical and graphical interpretation methodologies. A PCA showed that Cl(-), Na+, Mg2+ and part of the K+ and SO4(2-) content were mainly contributed by marine aerosols, whereas the contribution from continental sources (combustion, traffic emissions and other urban activities) was dominant in the content of NO3(-) and part of the SO4(2-) and Mn content. Soil resuspension was responsible for the concentrations of most of the trace metals (apart from Mn) and Ca2+ in the rainwater. An inverse correlation among the elemental concentrations, amount of rainfall and wind speed was observed. The northern transport pathway was identified as being associated with high concentrations of NO3(-) and slightly decreased pH values. However, the low standard deviation observed for the pH values during the sampling campaign also showed a small variation in the data, suggesting that the acidity is most probably being constantly sourced from a natural origin, such as organic acids.

  11. Antimicrobial resistance and PCR-ribotyping of Shigella responsible for foodborne outbreaks occurred in southern Brazil

    PubMed Central

    de Paula¹, Cheila Minéia Daniel; Mercedes, Passos Geimba²; do Amaral¹, Patrícia Heidrich; Tondo, Eduardo Cesar

    2010-01-01

    Little information about Shigella responsible for foodborne shigellosis is available in Brazil. The present study aimed to investigate the antimicrobial resistance and PCR-ribotyping patterns of Shigella isolates responsible for foodborne outbreaks occurred in Rio Grande do Sul State (RS), Southern Brazil in the period between 2003 and 2007. Shigella strains (n=152) were isolated from foods and fecal samples of victims of shigellosis outbreaks investigated by the Surveillance Service. Identification of the strains at specie level indicated that 71.1% of them were S. flexneri, 21.5% S. sonnei, and 0.7% S. dysenteriae. Ten strains (6.7%) were identified only as Shigella spp. An increasing occurrence of S. sonnei was observed after 2004. Most of the strains were resistant to streptomycin (88.6%), followed by ampicillin (84.6%), and sulfamethoxazole/trimethoprim (80.5 %). Resistant strains belonged to 73 patterns, and pattern A (resistance to ampicillin, sulfamethoxazole/trimethoprim, tetracycline, streptomycin, chloramphenicol, and intermediate resistance to kanamycin) grouped the largest number of isolates (n=36). PCR-ribotyping identified three banding patterns (SH1, SH2, and SH3). SH1 grouped all S. flexneri and SH2 grouped all S. sonnei. The S. dysenteriae strain belonged to group SH3. According to the results, several Shigella isolates shared the same PCR-rybotyping banding pattern and the same resistance profile, suggesting that closely related strains were responsible for the outbreaks. However, other molecular typing methods need to be applied to confirm the clonal relationship of these isolates. PMID:24031576

  12. Modeling sugarcane ripening as a function of accumulated rainfall in Southern Brazil.

    PubMed

    Cardozo, Nilceu P; Sentelhas, Paulo C; Panosso, Alan R; Palhares, Antonio L; Ide, Bernardo Y

    2015-12-01

    The effect of weather variables on sugarcane ripening is a process still not completely understood, despite its huge impact on the quality of raw material for the sugar energy industry. The aim of the present study was to evaluate the influence of weather variables on sugarcane ripening in southern Brazil, propose empirical models for estimating total recoverable sugar (TRS) content, and evaluate the performance of these models with experimental and commercial independent data from different regions. A field experiment was carried out in Piracicaba, in the state of São Paulo, Brazil, considering eight sugarcane cultivars planted monthly, from March to October 2002. In 2003, at the harvest, 12 months later, samples were collected to evaluate TRS (kg t(-1)). TRS and weather variables (air temperature, solar radiation, relative humidity, and rainfall) were analyzed using descriptive and multivariate statistical analysis to understand their interactions. From these correlations, variables were selected to generate empirical models for estimating TRS, according to the cultivar groups and their ripening characteristics (early, mid, and late). These models were evaluated by residual analysis and regression analysis with independent experimental data from two other locations in the same years and with independent commercial data from six different locations from 2005 to 2010. The best performances were found with exponential models which considered cumulative rainfall during the 120 days before harvest as an independent variable (R (2) adj ranging from 0.92 to 0.95). Independent evaluations revealed that our models were capable of estimating TRS with reasonable to high precision (R (2) adj ranging from 0.66 to 0.99) and accuracy (D index ranging from 0.90 to 0.99), and with low mean absolute percentage errors (MAPE ≤ 5 %), even in regions with different climatic conditions.

  13. Side effects and discontinuation of oral contraceptive use in southern Brazil.

    PubMed

    Janowitz, B; Kane, T T; Arruda, J M; Covington, D L; Morris, L

    1986-07-01

    Oral contraceptives have many advantages, but sometimes also have side effects which can cause users to switch appropriately or inappropriately to less effective methods or abandon contraception. In Brazil, 2/3 of married women of childbearing age were using contraception in 1981, and 1/2 of these were using orals. Contraceptive behavior following reported side effects in users of oral contraceptives in Southern Brazil is examined in this study, in relation to diverse factors. Among 2904 currently-married women, aged 15-44, almost 75% reported that they had used the pill at some time, and of these, 45.6% were still doing so. Data on perceived side effects were gathered for all women. There was no independent medical evaluation of the effects, so the data did not necessarily represent actual prevalence of pill related problems. Women who reported problems with the pill were less likely to be current users (25%) than women who did not (65%). However, overall contraceptive prevalence was about the same in both groups (66.2% and 67.0% respectively), indicating that women who stop using oral contraceptives usually switch to another method. However, they are more likely to be using traditional methods than women in the general population, especially if they want more children. Termination of pill use varies little according to the type of problem reported. Women with problems who sought medical attention were more likely to stop using the pill, and 82.4% of women advised to stop by their physician did so, but the major factor affecting discontinuation was the reported experience of a problem. The most frequently reported problems were headaches (38.1%), nausea (34.1%), nervousness (27.9%), and vertigo (18.3%). Physician intervention should help to avoid women's abandoning oral contraceptives unnecessarily.

  14. Salmonelloses in the State of Rio Grande do Sul, southern Brazil, 2002 to 2004

    PubMed Central

    Wagner, Vanessa Rech; Silveira, Josete Baialardi; Tondo, Eduardo Cesar

    2013-01-01

    Salmonella has been identified as the main aetiological agent responsible for foodborne diseases in several countries worldwide, including Brazil. In the State of Rio Grande do Sul (RS), southern Brazil, previews studies analysed official foodborne illnesses data, identifying Salmonella as the main bacterial agent of foodborne diseases during the period of 1997 to 2001. The present study aimed to analyse the official epidemiological data on salmonelloses occurred in the State of RS, during the period of 2002 to 2004. Even though data on recent salmonelloses were available, only data concerning the period comprising in 2002 to 2004 were analysed because the official worksheet records presented more consistent information about the salmonellosis outbreaks. Results indicated that, among the 624 foodborne outbreaks officially investigated, 202 (32.37%) were confirmed as salmonellosis. Among them 23,725 people were involved, 4,148 became sick, 1,878 were hospitalized and one person died. The season with the highest incidence of salmonelloses was spring, and the most affected age group was composed of people aged between 20 to 49 years old (56.66%). Animal origin foods - especially eggs and meat products - were very often involved with the outbreaks, however homemade mayonnaise was identified as the main food vehicle for salmonelloses (53.51%). The majority of the cases occurred inside private homes (55.81%) and food services (12.1%), and the main factors contributing to the occurrence of the outbreaks were the consumption of products without sanitary inspection (26.7%) and exposure of food at room temperature for more than two hours (18.58%). Similarly to what was previously reported for the period of 1997 to 2001, Salmonella spp. was the most prevalent foodborne disease agent in the State of RS during the years of 2002 to 2004. PMID:24516439

  15. High occurrence of Calodium hepaticum (syn. Capillaria hepatica) spurious infection in a village in the Atlantic Forest of southern Brazil

    PubMed Central

    Klisiowicz, Débora do Rocio; Reifur, Larissa; Shimada, Márcia Kiyoe; Haidamak, Juciliane; Cognialli, Regielly Caroline Raimundo; Ferreira, Tatiane

    2014-01-01

    Calodium hepaticum (syn. Capillaria hepatica) is a nematode of the Capillariidae family that infects rodents and other mammals. In Brazil, human spurious infections of C. hepaticum have been detected in indigenous or rural communities from the Amazon Basin, but not in the southern states of the country. Here, we report the highest occurrence (13.5% of 37 residents) of C. hepaticum human spurious infection detected in Brazil and the first record in a southern region, Guaraqueçaba. The finding is explained by the area being located in the Atlantic Forest of the state of Paraná, surrounded by preserved forests and because the inhabitants consume the meat of wild mammals. PMID:24676661

  16. A new species of inseminating seasonal killifish of the Cynopoecilus melanotaenia complex from southern Brazil (Cyprinodontiformes: Rivulidae)

    PubMed Central

    Amorim, Pedro F

    2016-01-01

    Abstract Background The Cynopoecilus melanotaenia complex is a morphologically homogeneous killifish group, endemic from an area encompassing southern Brazil and northeastern Uruguay. It presently comprises four valid species: C. melanotaenia, the type species of the genus, and C. fulgens, C. intimus, and C. nigrovittatus. New information Cynopoecilus feltrini, n. sp., from the lower Tubarão river basin, southern Brazil, is distinguished from all congeners of the C. melanotaenia complex by having frontal E-scales medially overlapped, branchiostegal region orangish red in males and dorsum with few dark brown spots above opercular region. A phylogenetic tree derived from the analysis of a fragment of the mitochondrial gene cytochrome c oxidase subunit I (681 bp) indicates that C. feltrini is a member of the clade that includes all species of the C. melanotaenia complex except C. melanotaenia, as well as that C. feltrini is the sister group of a clade comprising C. fulgens and C. nigrovittatus. PMID:27099548

  17. Three new species of mealybug (Hemiptera, Coccomorpha, Pseudococcidae) on persimmon fruit trees (Diospyros kaki) in southern Brazil.

    PubMed

    Pacheco da Silva, Vitor C; Kaydan, Mehmet Bora; Germain, Jean-François; Malausa, Thibaut; Botton, Marcos

    2016-01-01

    Brazil has the greatest insect diversity in the world; however, little is known about its scale insect species (Hemiptera: Coccomorpha). Mealybugs (Pseudococcidae) have been found in at least 50% of persimmon orchards Diospyros kaki L. in the southern part of the country. In this study three new mealybug species on persimmon trees located in the Serra Gaúcha Region, RS, Brazil, namely, Anisococcus granarae Pacheco da Silva & Kaydan, sp. n., Ferrisia kaki Kaydan & Pacheco da Silva, sp. n. and Pseudococcus rosangelae Pacheco da Silva & Kaydan, sp. n. are described. In addition, an identification key for the genera occurring on fruit orchards and vineyards in Brazil is provided, together with illustrations and molecular data for the new species.

  18. Three new species of mealybug (Hemiptera, Coccomorpha, Pseudococcidae) on persimmon fruit trees (Diospyros kaki) in southern Brazil

    PubMed Central

    Pacheco da Silva, Vitor C.; Kaydan, Mehmet Bora; Germain, Jean-François; Malausa, Thibaut; Botton, Marcos

    2016-01-01

    Abstract Brazil has the greatest insect diversity in the world; however, little is known about its scale insect species (Hemiptera: Coccomorpha). Mealybugs (Pseudococcidae) have been found in at least 50% of persimmon orchards Diospyros kaki L. in the southern part of the country. In this study three new mealybug species on persimmon trees located in the Serra Gaúcha Region, RS, Brazil, namely, Anisococcus granarae Pacheco da Silva & Kaydan, sp. n., Ferrisia kaki Kaydan & Pacheco da Silva, sp. n. and Pseudococcus rosangelae Pacheco da Silva & Kaydan, sp. n. are described. In addition, an identification key for the genera occurring on fruit orchards and vineyards in Brazil is provided, together with illustrations and molecular data for the new species. PMID:27199595

  19. Treatment and Follow-up of Human Papillomavirus Infected Women in a Municipality in Southern Brazil.

    PubMed

    Ruggeri, Joao Batista; Dell Agnolo, Catia Millene; Gravena, Angela Andreia Franca; de Oliveira Demitto, Marcela; Lopes, Tiara Cristina Romeiro; Delatorre, Silvana; de Barros Carvalho, Maria Dalva; Consolaro, Marcia Edilaine Lopes; Pelloso, Sandra Marisa

    2015-01-01

    This study aimed toanalyze the risk behavior for cervical cancer (CC) and the human papillomavirus (HPV) prevalence and resolution among women who received care through the private healthcare network of a municipality in southern Brazil. This descriptive and retrospective study was conducted with 25 women aged 20 to 59 years who received care through the private healthcare network and were treated at a specialty clinic in the period from January to December 2012 in a municipality in Northwest Parana, Southern Brazil. Data from medical records with cytological and HPV results were used. Following treatment, these women were followed-up and reassessed after 6 months. Data were statistically analyzed using the t-test and chi-squared test at a 5% significance level. The mean age of the studied women was 27.8±7.75 years old, and the majority were married, with paid employment and were non-smokers. The mean age at menarche was 13.0±0.50 years old, and the mean age at first intercourse was 17.5±1.78 years, with only 8.0% (2) initiating sexual activity at an age ≤15 years old. The majority had 1 to 2 children (60.0%), while 88.0% reported having had one sexual partner in their lifetime, and all the women were sexually active. A total of 68.0% used a hormonal contraceptive method. All the women had leukorrhea and pain and were infected by a single HPV type. Regarding the lesion grade, 80.0% showed high risk and 20.0% low risk. The most prevalent high-risk HPV strain was 16. These findings provide relevant information on HPV risk factors and infection, as well as the treatment and 6-month follow-up results, in economically and socially advantaged women with no traditional risk factors, corroborating previous reports that different risk factors may be described in different populations. Thus, this study reinforces the fact that even women without the traditional risk factors should undergo HPVmonitoring and assessment to determine the persistence of infection, promoting

  20. Sea surface temperature variability off southern Brazil and Uruguay as revealed from historical data since 1854

    NASA Astrophysics Data System (ADS)

    Zavialov, Peter O.; Wainer, Ilana; Absy, JoãO. M.

    1999-09-01

    About 300,000 quality-controlled local reports from ships of opportunity were complemented with the data extracted from global data records to compile monthly series of sea surface temperature (SST) for the period 1854 to 1994 on a grid 1° × 1° in latitude and longitude. These historical data are used to investigate the variability off the coast of southern Brazil and Uruguay in a broad range of temporal scales from seasonal to secular. With respect to behavior at these scales, three distinct areas can be identified in the study region. The first one, located over the shelf and controlled by winter invasions of subantarctic water along with Rio de la Plata and Patos-Mirim discharges, is characterized by large annual range of SST (7° to over 10°C), energetic mean square variability (from 1.4 to 2.2°C2, after removal of seasonal signal), and an extremely high secular trend toward warming (1.2 to 1.6°C per 100 years), especially in the proximity of the estuaries. The second one, an area of the Brazil Current influence, exhibits smaller annual range (5° to 7°C) and mean square variability (1 to 1.4°C2). The secular trend is from 1° to 1.2°C per 100 years, smaller than observed in the shelf, but still high compared to the global average. The third area, which encompasses the eastern deep ocean part of the region away from the influence of either major currents or coastal discharges, exhibits less energetic variability at all examined scales, as compared to the rest of the region. Everywhere in the region, 50 to 80% of interannual variability is associated with periods smaller than 10 years; however, compared to the rest of the region, the shelf zone is characterized by a relatively large contribution from decadal and interdecadal scales. In austral winter a thermal front forms in the study region, separating warm tropical water associated with the Brazil Current and cold subantarctic water flowing northward on the shelf with an admixture of coastal

  1. Norovirus Recombinant Strains Isolated from Gastroenteritis Outbreaks in Southern Brazil, 2004–2011

    PubMed Central

    Leite, José Paulo Gagliardi; Miagostovich, Marize Pereira

    2016-01-01

    Noroviruses are recognized as one of the leading causes of viral acute gastroenteritis, responsible for almost 50% of acute gastroenteritis outbreaks worldwide. The positive single-strand RNA genome of noroviruses presents a high mutation rate and these viruses are constantly evolving by nucleotide mutation and genome recombination. Norovirus recombinant strains have been detected as causing acute gastroenteritis outbreaks in several countries. However, in Brazil, only one report of a norovirus recombinant strain (GII.P7/GII.20) has been described in the northern region so far. For this study, 38 norovirus strains representative of outbreaks, 11 GII.4 and 27 non-GII.4, were randomly selected and amplified at the ORF1/ORF2 junction. Genetic recombination was identified by constructing phylogenetic trees of the polymerase and capsid genes, and further SimPlot and Bootscan analysis of the ORF1/ORF2 overlap. Sequence analysis revealed that 23 out of 27 (85%) non-GII.4 noroviruses were recombinant strains, characterized as: GII.P7/GII.6 (n = 9); GIIP.g/GII.12 (n = 4); GII.P16/GII.3 (n = 4); GII.Pe/GII.17 (n = 2); GII.P7/GII.14 (n = 1); GII.P13/GII.17 (n = 1); GII.P21/GII.3 (n = 1); and GII.P21/GII.13 (n = 1). On the other hand, among the GII.4 variants analyzed (Den Haag_2006b and New Orleans_2009) no recombination was observed. These data revealed the great diversity of norovirus recombinant strains associated with outbreaks, and describe for the first time these recombinant types circulating in Brazil. Our results obtained in southern Brazil corroborate the previous report for the northern region, demonstrating that norovirus recombinant strains are circulating more frequently than we expected. In addition, these results emphasize the relevance of including ORF1/ORF2-based analysis in surveillance studies as well as the importance of characterizing strains from other Brazilian regions to obtain epidemiological data for norovirus recombinant strains circulating in the

  2. Vocalizations produced by southern right whale (Eubalaena australis) mother-calf pairs in a calving ground off Brazil.

    PubMed

    Dombroski, Julia R G; Parks, Susan E; Groch, Karina R; Flores, Paulo A C; Sousa-Lima, Renata S

    2016-09-01

    Aiming to gather information on southern right whale (Eubalaena australis) mother-calf pairs' vocal behavior, archival acoustic recorders were deployed at a calving area off Brazil. Manual inspection of spectrograms revealed seven call classes: upcall, downcall, down-upcall, tonal variable, tonal constant, hybrid, and pulsive calls, which are consistent with those previously described for this species in Argentina. Gunshots and warbles, vocalizations described from other right whale species, were not detected. Mean values of start, end, maximum, minimum and peak frequencies, frequency bandwidth and duration were calculated for each call class. Start and end frequencies, frequency bandwidth and duration of upcalls recorded off Brazil were compared to those from other right whale populations and species. Only mean duration of upcalls from Brazil were significantly different from upcalls from all other populations. Differences in call duration may be driven by differences in demographic factors or background noise features among study areas. The repertoire characterization presented in this study will contribute to increase the utility of passive acoustic monitoring as a tool for conservation and research of southern right whales off Brazil as it provides important baseline information on the vocal behavior of this species.

  3. Holocene environmental changes in the São Francisco de Paula region, southern Brazil

    NASA Astrophysics Data System (ADS)

    Behling, Hermann; Bauermann, Soraia Girardi; Pereira Neves, Paulo César

    2001-11-01

    Holocene paleoenvironments have been interpreted from a radiocarbon dated pollen and charcoal record of the São Francisco de Paula region on the southern Brazilian highland. Today the region is characterized by pastureland and small patches of disturbed Araucaria forest. The region receives 2450 mm per annum. This is the highest precipitation rate in southern Brazil. Studied surface samples represent the modern pollen analog of the anthropogenic influenced vegetation. Pollen analytical studies of the 84 cm long core, collected from a small basin with clay, organic matter and peat deposits, show that pollen and spores of the lower core section (84 and 46 cm depth) have been almost destroyed. This period between ca. 7500 and 4000 14C yr BP was too dry for conservation of pollen and spores in the basin. Climate must have been markedly drier than today during early and mid Holocene times. After 4000 14C yr BP, preserved pollen grains reflect wetter conditions than before and indicate the predominance of campos (grassland) vegetation with small areas of Araucaria forest in the study region. Forest expansion is documented since 1060 14C yr BP and expansion of Araucaria angustifolia trees itself since 850 14C yr BP. During the last 1000 yr, rainfall must have been much more intensive with no or only short dry periods such as the modern climate. The results confirm the vegetational and climatic changes documented from the Araucaria forest region of Santa Catarina and Paraná State. Concentration and accumulation rates of carbonized particles are somewhat higher during the last 850 14C yr BP than before, indicating an increased fire frequency.

  4. Local distribution and abundance of Cardisoma guanhumi Latreille, 1928 (Brachyura: Gecarcinidae) in southern Brazil.

    PubMed

    Oliveira-Neto, J F; Batista, E; Metri, R; Metri, C B

    2014-02-01

    The blue land crab, Cardisoma guanhumi Latreille, 1828 (Brachyura: Gecarcinidae) is officially included in the list of over-exploited species in Brazil, although still abundantly found in the state of Santa Catarina, the southern limit of its distribution. This species was found in forested areas, gardens, and grassy areas, including crabs with carapace width larger than 80mm. The existence of this population with these characteristics is surprising, since there is only one official record of the species in the southern region. The objectives of this study are to estimate the abundance and occupation patterns of C. guanhumi in this region. Correlations with conservation were discussed. The absolute abundance of crabs in the middle of summer activity was established for an area of 100,000 m2. A smaller area was mapped and divided into sampling units for statistical analyses. We distributed approximately 240 crabs in a forested area of about 3,000 m2 and 150 crabs in grassy areas (90,000 m2). The statistical test of Kruskal-Wallis test showed that there are significant differences between the sizes of the openings of the galleries inside the forest and that located in grassy areas. In the forest, the openings tend to be much larger. Burrows were found at a distance of 150 metres from the channel. The number of galleries was higher in the forested area, although the burrows were more densely grouped in grassy areas. Although C. guanhumi seems to be adjusting well to changes caused by human occupation, small forested areas are more conducive to growth and conservation of this species.

  5. Occurrence of mycotoxins in wheat grains exposed to fungicides on fusarium head blight control in southern Brazil.

    PubMed

    Marques, Leandro N; Pizzutti, Ionara R; Balardin, Ricardo S; Dos Santos, Ingrid D; Dias, Jonatan V; Stefanello, Marlon T; Serafini, Pablo T

    2017-01-12

    Mycotoxins occurrence in wheat grains impose risks to human and animal health. The southern Brazil has favorable weather conditions for Fusarium graminearum infections and consequently for mycotoxins accumulation on grains. The goal of this study was to evaluate the behavior of new wheat commercial genotypes to Fusarium Head Blight (FHB), to control performance of new fungicide formulations and their relationship with mycotoxins concentration in grains. The manly mycotoxin occurrence on wheat grains in southern Brazil was deoxynivalenol (DON). Two cultivars showed high DON concentration above the tolerance limits (>3000 μg kg(-1)). Many other mycotoxins monitored presented concentrations below method detection limit. Satisfactory levels of fungicide effectiveness were achieved against F. graminearum. Some fungicides promoted a satisfactory decrease on DON accumulation in grains. The best results were obtained when prothioconazole was present. SDHI (Succinate dehydrogenase inhibitors) + QoI (Quinone outside inhibitors) fungicides showed benefic effects at FHB control at field, but it did not promote satisfactory reduction on DON contamination. Fungicides can be used satisfactory for FHB control and reduce DON contamination in grains in southern Brazil. The presence of prothioconazole should be recommended. Some genotypes showed high DON concentration and it was not directly related with FHB severity at field.

  6. Diversity and distribution of aquatic insects in Southern Brazil wetlands: implications for biodiversity conservation in a Neotropical region.

    PubMed

    Maltchik, Leonardo; Dalzochio, Marina Schmidt; Stenert, Cristina; Rolon, Ana Silvia

    2012-03-01

    The selection of priority areas is an enormous challenge for biodiversity conservation. Some biogeographic methods have been used to identify the priority areas to conservation, and panbiogeography is one of them. This study aimed at the utilization of panbiogeographic tools, to identify the distribution patterns of aquatic insect genera, in wetland systems of an extensive area in the Neotropical region (approximately 280 000km2), and to compare the distribution of the biogeographic units identified by the aquatic insects, with the conservation units of Southern Brazil. We analyzed the distribution pattern of 82 genera distributed in four orders of aquatic insects (Diptera, Odonata, Ephemeroptera and Trichoptera) in Southern Brazil wetlands. Therefore, 32 biogeographic nodes corresponded to the priority areas for conservation of the aquatic insect diversity. Among this total, 13 were located in the Atlantic Rainforest, 16 in the Pampa and three amongst both biomes. The distribution of nodes showed that only 15% of the dispersion centers of insects were inserted in conservation units. The four priority areas pointed by node cluster criterion must be considered in further inclusions of areas for biodiversity conservation in Southern Brazil wetlands, since such areas present species from different ancestral biota. The inclusion of such areas into the conservation units would be a strong way to conserve the aquatic biodiversity in this region.

  7. On the fossil remains of Panochthus Burmeister, 1866 (Xenarthra, Cingulata, Glyptodontidae) from the Pleistocene of southern Brazil.

    PubMed

    Ferreira, José D; Zamorano, Martín; Ribeiro, Ana Maria

    2015-03-01

    The genus Panochthus represents the last lineage of "Panochthini" recorded in the Pleistocene. This genus has a wide latitudinal distribution in South America, and in Brazil it occurs in the southern and northeastern regions. In this paper we describe new material (isolated osteoderms and caudal tube fragments) assigned to Panochthus from the state of Rio Grande do Sul (southern Brazil) and discuss some taxonomic issues related to Panochthus tuberculatus and Panochthus greslebini based on this material . The occurrence of P. greslebini is the first for outside the Brazilian Intertropical Region. In addition, we describe new diagnostic features to differentiate the osteoderms of P. greslebini and P. tuberculatus. Unfortunately, it was not possible to identify some osteoderms at the species level. Interestingly, they showed four distinct morphotypes characterized by their external morphology, and thus were attributed to Panochthus sp. Lastly, we conclude that in addition to P.tuberculatus registered to southern Brazil, there is another species of the genus, assignable to P. cf. P. greslebini. Our analysis reinforce the reliability of caudal tube characters for the classification of species of Panochthus.

  8. The origin of fluoride in the granitic aquifer of Porto Alegre, Southern Brazil

    NASA Astrophysics Data System (ADS)

    Viero, A. P.; Roisenberg, C.; Roisenberg, A.; Vigo, A.

    2009-02-01

    The Porto Alegre region, Southern Brazil, comprises a fractured aquifer system constituted by pre-Cambrian granitic and gneissic rocks, and a porous aquifer system formed by Cenozoic muddy-sandy to sandy sediments. A model is presented for the origin of the fluoride in the groundwater of the fractured aquifer system, based on ionic interrelations, a statistical analysis of physicochemical parameters and geochemical modeling. The fluoride is present at levels of up to 6.13 mg/L in groundwater and it arises due to the dissolution of secondary fluorite filling fractures in the granitic and gneissic rocks. The dissolution of fluorite occurs at the same time as calcite and dolomite solubilization. The statistical analysis identified three chemically distinct groups (named 1, 2 and 3) and two subgroups (1A and 1B) of groundwater in the fractured aquifer. The most significant differences between these groups are the different concentration ranges of fluoride, calcium, bicarbonate, magnesium, total dissolved solids and chloride and pH values, as well as the correlations between them. The compositional evolution of each groundwater group is governed mainly by how much high-salinity groundwater from the porous aquifer system is mixed with them and the different thermodynamic equilibrium conditions of calcite, dolomite and fluorite.

  9. Genetic structure of drone congregation areas of Africanized honeybees in southern Brazil

    PubMed Central

    2009-01-01

    As yet, certain aspects of the Africanization process are not well understood, for example, the reproductive behavior of African and European honeybees and how the first Africanized swarms were formed and spread. Drone congregation areas (DCAs) are the ideal place to study honeybee reproduction under natural conditions since hundreds of drones from various colonies gather together in the same geographical area for mating. In the present study, we assessed the genetic structure of seven drone congregations and four commercial European-derived and Africanized apiaries in southern Brazil, employing seven microsatellite loci for this purpose. We also estimated the number of mother-colonies that drones of a specific DCA originated from. Pairwise comparison failed to reveal any population sub-structuring among the DCAs, thus indicating low mutual genetic differentiation. We also observed high genetic similarity between colonies of commercial apiaries and DCAs, besides a slight contribution from a European-derived apiary to a DCA formed nearby. Africanized DCAs seem to have a somewhat different genetic structure when compared to the European. PMID:21637465

  10. Evaluation of the TLC quantification method and occurrence of deoxynivalenol in wheat flour of southern Brazil.

    PubMed

    Rocha, Denise Felippin de Lima; Oliveira, Melissa Dos Santos; Furlong, Eliana Badiale; Junges, Alexander; Paroul, Natalia; Valduga, Eunice; Backes, Geciane Toniazzo; Zeni, Jamile; Cansian, Rogério Luis

    2017-09-07

    The study evaluated a QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) extraction method for use with a TLC quantification procedure for deoxynivalenol (DON). It also surveyed DON occurrence in wheat flour from the southern region of Brazil. Forty-eight wheat flour samples were analysed, divided into 2 different harvest lots, each consisting of 24 different brands. The detection and quantification limits of the method were 30 and 100 ng of DON on the TLC plate. The various concentrations of DON presented high linearity (R(2) = 0.99). A negative matrix effect (-28%) of the wheat flour was verified, with suppression of the chromatographic signal of DON, and 80.2-105.4% recovery. The TLC method was reliable for DON evaluation, with a coefficient of variation of less than 10%. High-performance liquid chromatography of lot 2 samples confirmed the presence of DON in all samples identified DON-positive by the TLC technique. Of the 48 wheat flour samples in lots 1 and 2 analysed by TLC, 33.3 and 45.8% of the samples respectively were above the Brazilian legislation limit. Correlations were observed between the water activity and DON content, and between the fungal count and moisture content of the wheat flours.

  11. Survey of spatial distribution of vector-borne disease in neighborhood dogs in southern Brazil

    PubMed Central

    Constantino, Caroline; de Paula, Edson Ferraz Evaristo; Brandão, Ana Pérola Drulla; Ferreira, Fernando; Vieira, Rafael Felipe da Costa; Biondo, Alexander Welker

    2017-01-01

    Neighborhood dogs may act as reservoirs and disseminators of vector-borne diseases in urban areas. Accordingly, the aim of this study was to ascertain the health status and the vector-borne pathogens infecting dogs living in public areas with high levels of human movement in the city of Curitiba, southern Brazil. Blood samples from 21 neighborhood dogs that were found in nine of 22 bus stations and two public parks were subjected to a complete blood cell (CBC) count, serum biochemical profiling, a commercial rapid ELISA test and a commercial real-time PCR panel of vector-borne diseases. The CBC count and serum biochemical profiling were within the normal range for dogs and only 1/21 (4.7%) of the dogs was seroreactive for Borrelia burgdorferi sensu stricto. The commercial real-time PCR panel showed that 7/21 (33.3%) of the dogs had Mycoplasma haemocanis infection, 9/21 (42.8%) had ‘Candidatus Mycoplasma haematoparvum’ and 4/21 (19.0%) had both. No statistical association between infected by the agents found here and abnormalities in physical examinations, laboratory tests or ectoparasite presence was found (p > 0.05). In conclusion, neighborhood dogs showed low prevalence of vector-borne diseases and satisfactory wellbeing, and dogs can be used as sentinels for disease exposure. PMID:28331833

  12. Human papillomavirus among women with atypical squamous cells of undetermined significance in southern Brazil.

    PubMed

    Paesi, Suelen; Correa, Leonardo; Tregnago, Márcia Caldart; Mandelli, Jovana; Roesch-Ely, Mariana

    2015-01-01

    To determine the prevalence of atypical squamous cells of undetermined significance (ASCUS) and human papillomavirus (HPV) genotypes in a population in southern Brazil. In a retrospective cross-sectional study, the prevalence of ASCUS was determined among women aged 20-60 years who were referred to a private medical center in Caxias do Sul by a gynecologist for assessment of a cervical condition between January 1, 2010, and September 30, 2011. Histologic and cytologic samples were tested for HPV, and polymerase chain reaction (PCR) was used to genotype any HPV DNA identified. Among the 250 included women, 25 (10.0%) had ASCUS. HPV DNA was found in 15 (60.0%) women with ASCUS and 115 (51.1%) of the 225 without ASCUS. Viral typing showed that 7 (46.7%) HPV-positive women with ASCUS had multiple infections with up to five different genotypes. Both low- and high-risk HPV genotypes were found in ASCUS samples; the most prevalent genotypes were HPV6/HPV11 (affecting 10 [66.7%] women), HPV51 (6 [40.0%]), and HPV16 (6 [40.0%]). ASCUS is not an indication of HPV infection. HPV screening and genotyping would benefit women with ASCUS, because treatment can be planned according to risk of carcinogenesis. Copyright © 2014 International Federation of Gynecology and Obstetrics. Published by Elsevier Ireland Ltd. All rights reserved.

  13. Taking pills for developmental ails in Southern Brazil: The biologization of adolescence?

    PubMed Central

    Béhague, Dominique P.

    2015-01-01

    In the late 1990s researchers in Pelotas Southern Brazil began documenting what they considered to be unacceptably high rates of licensed psychotropic use among individuals of all ages, including youth. This came as a surprise, since the vast majority of psychiatrists in Pelotas draw on psychoanalytic theory and approach pharmaceutical use, especially for children and adolescents, in a consciously tempered way. Drawing from a longitudinal ethnographic sub-study, part of a larger 1982 birth cohort study, this paper follows the circuitous trajectories of emergent pharma-patterns among “shantytown” youth over a ten-year period, exploring the thickly layered and often moralized contingencies in which psychodynamic psychiatrists' intention to resist excessive pharmaceuticalization both succeed and crumble. I juxtapose these trajectories with the growing salience of an “anti-biologizing” explanatory framework that psychiatrists and researchers are using to pre-empt the kind of diagnostics-driven “biopsychiatrization” so prevalent in North America. My analysis suggests that psychiatrists' use of this framework ironically contributes to their failed attempts to “resist” pharmaceuticalization. PMID:25533870

  14. The ichthyofauna of limnic systems in Quaternary deposits of extreme southern Brazil

    PubMed Central

    Assumpção, Cindy M.; Quintela, Fernando M.; Corrêa, Fabiano; Loebmann, Daniel

    2016-01-01

    Abstract The Quaternary in the state of Rio Grande do Sul (RS), southern Brazil, is geologically represented by the coastal plain and was originated by successive events of Pleistocene-Holocene marine transgressions and the occurrence of alluvial deposits. This paper aimed to characterize the fish assemblage occurring in a swampy Quaternary area adjacent to Lagoa Pequena, a lacustrine system connected to the west margin of the Laguna dos Patos estuary. A checklist is also provided of the ichthyofauna so far recorded in limnic systems of Quaternary deposits in the state of Rio Grande do Sul. A total of 42 species was recorded, distributed in nine orders, 18 families and 31 genera. Characidae and Cichlidae were the most representative families, comprising 15 and 4 species respectively. A bibliographic revision associated to our sample data revealed the occurrence of 156 species in limnic systems inserted in RS Quaternary deposits (114 limnic, 15 marine/estuarine/limnic, ten marine/estuarine, nine estuarine/limnic and eight marine). Characiformes and Siluriformes are the most diverse orders, corroborating the Neotropical pattern. Seven species can be considered endemic to RS Quaternary deposits. PMID:28174498

  15. Seasonal patterns of horse fly richness and abundance in the Pampa biome of southern Brazil.

    PubMed

    Krüger, Rodrigo Ferreira; Krolow, Tiago Kütter

    2015-12-01

    Fluctuations in seasonal patterns of horse fly populations were examined in rainforests of tropical South America, where the climate is seasonal. These patterns were evaluated with robust analytical models rather than identifying the main factors that influenced the fluctuations. We examined the seasonality of populations of horse flies in fields and lowland areas of the Pampa biome of southern Brazil with generalized linear models. We also investigated the diversity of these flies and the sampling effort of Malaise traps in this biome over two years. All of the 29 species had clear seasonality with regard to occurrence and abundance, but only seven species were identified as being influenced by temperature and humidity. The sampling was sufficient and the estimated diversity was 10% more than observed. Seasonal trends were synchronized across species and the populations were most abundant between September and March and nearly zero in other months. While previous studies demonstrated that seasonal patterns in population fluctuations are correlated with climatic conditions in horse fly assemblages in South America rainforests, we show a clear effect of each factor on richness and abundance and the seasonality in the prevalence of horse fly assemblages in localities of the Pampa biome. © 2015 The Society for Vector Ecology.

  16. Ecological risk analysis of pesticides used on irrigated rice crops in southern Brazil.

    PubMed

    Vieira, Danielle Cristina; Noldin, José Alberto; Deschamps, Francisco C; Resgalla, Charrid

    2016-11-01

    Based on studies conducted in the past decade in the southern region of Brazil to determine residue levels of the pesticides normally used on irrigated rice crops, changes can be observed in relation to the presence of pesticides in the waters of the main river basins in Santa Catarina State. In previous harvests, the presence of residues of 7 pesticides was determined, with the herbicide bentazon and the insecticide carbofuran being the products showing highest frequency. Following toxicological tests conducted with 8 different test organisms, deterministic and probabilistic risk analysis was performed to assess the situation of the river basins in areas used for the production of irrigated rice. Of the species tested, the herbicide bentazon showed greatest toxicity toward plants, but did not present an ecological risk because in the worst-case scenario the highest concentration of this pesticide in the environment is 37 times lower than the lowest EC50/LC50 value obtained in the tests. The insecticide carbofuran, which had the highest toxicity toward the organisms used in the tests, presented an ecological risk in the deterministic analysis, but without any associated probability. The results highlight the need for increased efforts in training farmers in crop management practices and for the continual monitor of water bodies for the presence of pesticide residues. Copyright © 2016 Elsevier Ltd. All rights reserved.

  17. Alteration in eating habits among shift workers of a poultry processing plant in southern Brazil.

    PubMed

    de Freitas, Elisângela da Silva; Canuto, Raquel; Henn, Ruth Liane; Olinto, Beatriz Anselmo; Macagnan, Jamile Block Araldi; Pattussi, Marcos Pascoal; Busnello, Fernanda Michelin; Olinto, Maria Teresa Anselmo

    2015-08-01

    The relationship between shift work and the eatinghabits of workers was investigated in a slaughterhouse in southern Brazil. It involved a cross-sectional study with 1,206 workers of both sexes between 18 and 50 years of age. A standardized questionnaire was used to gather demographic, socioeconomic, work shift and eating habit information. The shift of work was categorized into daytime and nighttime, based on the starting and ending times of the shift. The eating habits of workers were evaluated as follows: number and type of meals eaten during the 24 hours of a normal day, the inappropriateness of the hoursof these meals and the dietaryrisk score. This was built on the risk score of the weekly consumption of 13 food items. After adjusting for potential confounders, non-Caucasian and younger male workers were more likely to manifest eating risk habits. Nighttimeshift workers consumed ahigher number of meals/day with greater inappropriateness of meal times than daytimeshift workers. The night shift can negatively influence the eating habits of workers of that shift.

  18. The ichthyofauna of limnic systems in Quaternary deposits of extreme southern Brazil.

    PubMed

    Assumpção, Cindy M; Quintela, Fernando M; Corrêa, Fabiano; Loebmann, Daniel

    2016-01-01

    The Quaternary in the state of Rio Grande do Sul (RS), southern Brazil, is geologically represented by the coastal plain and was originated by successive events of Pleistocene-Holocene marine transgressions and the occurrence of alluvial deposits. This paper aimed to characterize the fish assemblage occurring in a swampy Quaternary area adjacent to Lagoa Pequena, a lacustrine system connected to the west margin of the Laguna dos Patos estuary. A checklist is also provided of the ichthyofauna so far recorded in limnic systems of Quaternary deposits in the state of Rio Grande do Sul. A total of 42 species was recorded, distributed in nine orders, 18 families and 31 genera. Characidae and Cichlidae were the most representative families, comprising 15 and 4 species respectively. A bibliographic revision associated to our sample data revealed the occurrence of 156 species in limnic systems inserted in RS Quaternary deposits (114 limnic, 15 marine/estuarine/limnic, ten marine/estuarine, nine estuarine/limnic and eight marine). Characiformes and Siluriformes are the most diverse orders, corroborating the Neotropical pattern. Seven species can be considered endemic to RS Quaternary deposits.

  19. Antiproliferative activity of the dimeric phloroglucinol and benzophenone derivatives of Hypericum spp. native to southern Brazil.

    PubMed

    Pinhatti, Amanda V; de Barros, Francisco M C; de Farias, Caroline B; Schwartsmann, Gilberto; Poser, Gilsane L von; Abujamra, Ana L

    2013-08-01

    A large number of plants are known to possess strong antitumor properties. Previous studies have verified the antiproliferative activity of the extracts and fractions from six species of Hypericum spp. growing in southern Brazil. In the present study, the in-vitro antiproliferative effects of two dimeric phloroglucinols (japonicin A and uliginosin B, isolated from Hypericum myrianthum) and two benzophenones (cariphenone A and cariphenone B, isolated from H. carinatum) were investigated against three tumor cell lines (HT-29 - human colon carcinoma cells; U-251 - human glioma cell line, and OVCAR-3 - human ovarian carcinoma cells). In addition, different doses of these compounds were associated with cytotoxic drugs commonly used as chemotherapy in the clinic. Cariphenone A and cariphenone B showed moderate antiproliferative activity against all tumor cell lines at a dose of 100 μg/ml. Unlike benzophenones, japonicin A and uliginosin B exerted antiproliferative effects only in the OVCAR-3 cell line. Moreover, a very strong synergistic effect was demonstrated by the association of subeffective doses of japonicin A with the chemotherapeutic drug paclitaxel, decreasing cellular proliferation of the OVCAR-3 cell line. These preliminary results provide a scientific basis to further pursue these compounds as potential combined therapy for certain tumor types.

  20. The correlation between breastfeeding self-efficacy and maternal postpartum depression in southern Brazil.

    PubMed

    Zubaran, Carlos; Foresti, Katia

    2013-03-01

    To investigate the relationship between breastfeeding self-efficacy and postpartum depression symptoms in a sample of Portuguese-speaking mothers in southern Brazil. There remains equivocal evidence regarding a putative association between breastfeeding self-efficacy and postpartum depression. This is a cross-sectional study in which eligible research participants completed screening questionnaires and other assessment tools. Mothers were interviewed once only in their homes between the 2nd and 12th week of the postpartum period. Research participants completed the Portuguese version of the Postpartum Depression Screening Scale (PDSS) and Edinburgh Postnatal Depression Scale (EPDS). Breastfeeding self-efficacy was evaluated through the Breastfeeding Self-Efficacy Scale (BSES-SF). A total number of 89 mothers completed the investigation: 69 (77%) were exclusively breastfeeding, whereas 20 mothers (22.7%) were partially breastfeeding at the time of the interview. Mothers who combined breastfeeding and bottle-feeding presented higher PDSS and EPDS scores. The breastfeeding self-efficacy scores were higher in mothers who exclusively breastfed and were negatively associated (p<0.001) with both EPDS and PDSS (postpartum depression) scores. These findings suggest that mothers who suffer from depressive symptoms may experience less confidence in their ability to breastfeed. This association may be particularly relevant for the purpose of screening procedures for depression and unsatisfactory breastfeeding during the postpartum period. Copyright © 2012 Elsevier B.V. All rights reserved.

  1. Anxiety symptoms in crack cocaine and inhalant users admitted to a psychiatric hospital in southern Brazil.

    PubMed

    Zubaran, Carlos; Foresti, Katia; Thorell, Mariana Rossi; Franceschini, Paulo Roberto

    2013-01-01

    The occurrence of psychiatric comorbidity among individuals with crack or inhalant dependence is frequently observed. The objective of this study was to investigate anxiety symptoms among crack cocaine and inhalant users in southern Brazil. The study investigated two groups of volunteers of equal size (n=50): one group consisted of crack cocaine users, and the other group consisted of inhalant users. Research volunteers completed the Portuguese versions of the State-Trait Anxiety Inventory (STAI), Hamilton Anxiety Rating Scale (HAM-A), and Self-Report Questionnaire (SRQ). Both crack and inhalant users experience significant symptoms of anxiety. Inhalant users presented significantly more anxiety symptoms than crack users according to the HAM-A questionnaire only. In contrast to the results of the HAM-A, the STAI failed to demonstrate a significant difference between the two groups of substance users. SRQ scores revealed that crack and inhalants users had significant degrees of morbidity. A significant difference regarding anxiety symptomatology, especially state anxiety, was observed among inhalant and crack users. Anxiety and overall mental psychopathology were significantly correlated in this sample. The results indicate that screening initiatives to detect anxiety and additional psychiatric comorbidities among crack and inhalant users are feasible and relevant. Copyright © 2013 Elsevier Editora Ltda. All rights reserved.

  2. Parasites of economically important bivalves from the southern coast of Bahia State, Brazil.

    PubMed

    Zeidan, Gabriela Calvi; Luz, Mariane Dos Santos Aguiar; Boehs, Guisla

    2012-01-01

    This study investigated the parasites of three commercially important bivalve species (Crassostrea rhizophorae, Mytella guyanensis and Lucina pectinata) from the southern coast of Bahia, Brazil. A total of 540 specimens were collected in August 2009 and February 2010, at three localities. The bivalve specimens were measured on their longest axis, opened, and macroscopically examined for the presence of parasites or signs of disease. They were then fixed in Davidson' solution and subjected to routine histological processing, with paraffin embedding and H&E staining; next, the specimens were examined under a light microscope. No parasites were observed associated with L. pectinata. Rickettsia-like organisms (RLOs), Sphenophrya sp. (Ciliophora), Nematopsis sp. (Apicomplexa), Urastoma sp. (Turbellaria) and Bucephalus sp. (Digenea) were observed in both C. rhizophorae and M. guyanensis, as well as Ancistrocoma sp. (Ciliophora) and Tylocephalum sp. (Cestoda) in the former. A high prevalence of Nematopsis sp. was seen, but caused no apparent damage to the host. Bucephalus sp. caused the destruction of tissues, with castration, but showed low prevalence. The other parasites occurred in low prevalence and intensity, without causing significant damage.

  3. Phenology of Guarea macrophylla Vahl (Meliaceae) in subtropical riparian forest in southern Brazil.

    PubMed

    Müller, A; Schmitt, J L

    2017-08-17

    Climate is one of the main factors that affect plant behavior. The phenology of Guarea macrophylla Vahl, which is a small tree used for reforestation of degraded areas, was monitored for 18 months in a riparian forest at the Schmidt Stream, Campo Bom, in the state of Rio Grande do Sul, southern Brazil. Vegetative (leaf fall and leaf flushing) and reproductive events were observed, with the latter divided into flowering (flower buds and anthesis) and fruiting (unripe, ripening and ripe fruit). Phenological events were related to temperature, photoperiod and precipitation and their seasonality was verified by circular statistical analysis. Vegetative phenophases were continuous; they were not related to climate factors and presented low intensity, emphasizing the perennial aspect of the species. Flowering occurred during spring and summer. Both flower buds and anthesis were related to temperature and photoperiod. Fruiting was constant and went through all stages of development. Unripe fruits developed during the months with the lowest photoperiod and ripen more intensely in winter, on colder days. Ripe fruit became available for dispersal in spring, in times of longer photoperiod and higher temperatures. Except for leaf fall, all other phenological events showed seasonality in their manifestation. The one-month difference between the onsets of the flowering phases observed in this study indicated that local climate changes induced the early occurrence of this phenophase.

  4. Inputs of heavy metals due to agrochemical use in tobacco fields in Brazil's Southern Region.

    PubMed

    Zoffoli, Hugo José Oliveira; do Amaral-Sobrinho, Nelson Moura Brasil; Zonta, Everaldo; Luisi, Marcus Vinícius; Marcon, Gracioso; Tolón-Becerra, Alfredo

    2013-03-01

    Only a few studies have assessed the joint incorporation of heavy metals into agricultural systems based on the range of agrochemicals used on a specific agricultural crop. This study was conducted to assess the heavy metals input through application of the main agrochemicals used in Brazilian tobacco fields. A total of 56 samples of different batches of 5 fertilizers, 3 substrates, 8 insecticides, 3 fungicides, 2 herbicides, and 1 growth regulator commonly used in the cultivation of tobacco in Brazil's Southern Region were collected from 3 warehouses located in the States of Rio Grande do Sul, Santa Catarina, and Paraná. The total As, Cd, Co, Cr, Cu, Hg, Fe, Mn, Ni, Pb, and Zn content of the samples was then determined and compared with the regulations of different countries and information found in the available literature. The fertilizers were identified as the primary source of heavy metals among the agrochemicals used. Application of pesticides directly to the shoots of tobacco plants contributed very little to the supply of heavy metals. The agrochemicals used in Brazilian tobacco fields provide lower inputs of the main heavy metals that are nonessential for plants than those registered in the international literature for the majority of crop fields in different regions of the world.

  5. Intoxication by Xanthium cavanillesii in cattle and sheep in southern Brazil.

    PubMed

    Mendez, M C; dos Santos, R C; Riet-Correa, F

    1998-06-01

    Three outbreaks of Xanthium cavanillesii intoxication occurred in the spring in the State of Rio Grande do Sul, southern Brazil. All outbreaks were observed in paddocks with sandy soils in flood plains along water streams. The paddocks had been under water, and after water withdrawal cattle were introduced to those areas which had seedlings of X cavanillesii and shortage of other forage. Mortality varied between 3 and 58%. Most animals were found dead or died a few hours after observation of clinical signs. The main lesion was a swollen liver with acute diffuse centrilobular necrosis. The disease was produced experimentally by feeding cotyledons at 0.75 and 1% bw in calves, and at 1.5 and 2% bw in sheep. Clinical signs and gross and microscopic lesions were similar to those observed in field cases. X cavanillesii in the 4-leaf stage with the attached cotyledons was also toxic for cattle and sheep, but the same plant without cotyledons lost its liver toxicity. The lethal dose of cotyledons divided in 5 daily doses was not toxic for cattle and sheep, demonstrating the non-cumulative effect of the plant.

  6. Application of agrometeorological spectral model in rice area in southern Brazil

    NASA Astrophysics Data System (ADS)

    Leivas, Janice F.; de C. Teixeira, Antonio Heriberto; Andrade, Ricardo G.; de C. Victoria, Daniel; Bayma-Silva, Gustavo; Bolfe, Edson L.

    2015-10-01

    The southern region is responsible for 70% of rice production in Brazil. In this study, rice areas of Rio Grande do Sul were selected, using the land use classification, scale 1: 100,000, provided by Brazilian Institute of Geography and Statistics (IBGE). MODIS Images were used and meteorological data, available by National Institute of Meteorology (INMET). The period of analysis was crop season 2011/2012, October to March. To obtain evapotranspiration was applied agrometeorological-spectral model SAFER (Simple Algorithm For Retrieving Evapotranspiration). From the analysis of the results, on planting and cultivation period , the average evapotranspiration (ET) daily was 1.93 +/- 0.96 mm.day-1. In the vegetative development period of rice, the daily ET has achieved 4.94 mm.day-1, with average value 2,31+/- 0.97 mm.day-1. In the period of harvest, evapotranspiration daily average was 1.84 +/- 0.80 mm.day-1. From results obtained, the estimation of evapotranspiration from satellite images may assist in monitoring the culture during the cycle, assisting in estimates of water productivity and crop yield.

  7. Feeding habits of coypu in the wetlands of the Southern region of Brazil.

    PubMed

    Colares, Ioni G; Oliveira, Raquel N V; Liveira, Rafael M; Colares, Elton P

    2010-09-01

    The aim of this study was to determine the diet of coypu (Myocastor coypus) in two areas (Santa Isabel do Sul wetlands - Area 1, and Santa Marta Farm - Area 2) in the Southern region of Brazil, using microhistological analyses of feces. Twenty-four plant species were identified from collected feces samples. Among the identified species, nine are common in the animals' diet in both areas; the presence of Oriza sativa was not detected in any of the samples. Among the identified species, 84% and 54% of the coypu's diet from Areas 1 and 2, respectively, are aquatic plants. Poaceae family was most common, being found in 82% of the samples from both areas. Paspalum disthichum (Area 1) and Panicum tricholaenoides (Area 2) were the most frequent species on the coypu's diet. The diet comparison for both areas indicates that feeding habits vary depending on the type of habitat, environmental conditions and food availability. The absence of rice in our analysis may be an indication for the preservation of native areas around watercourses, so as to prevent coypu from invading irrigated crops.

  8. Risk communication concerning welding fumes for the primary preventive care of welding apprentices in southern Brazil.

    PubMed

    Cezar-Vaz, Marta Regina; Bonow, Clarice Alves; Vaz, Joana Cezar

    2015-01-19

    This study's aim was to assess the perceptions of welding apprentices concerning welding fumes being associated with respiratory and cardiovascular disorders and assess the implementation of risk communication as a primary prevention tool in the welding training process. This quasi-experimental, non-randomized study with before-and-after design was conducted with 84 welding apprentices in Southern Brazil. Poisson Regression analysis was used. Relative Risk was the measure used with a 95% confidence interval and 5% (p ≤ 0.05) significance level. Significant association was found between perceptions of worsened symptoms of respiratory disorders caused by welding fumes and educational level (p = 0.049), the use of goggles to protect against ultraviolet rays (p = 0.023), and access to services in private health facilities without insurance coverage (p = 0.001). Apprentices younger than 25 years old were 4.9 times more likely to perceive worsened cardiovascular symptoms caused by welding fumes after risk communication (RR = 4.91; CI 95%: 1.09 to 22.2). The conclusion is that risk communication as a primary preventive measure in continuing education processes implemented among apprentices, who are future welders, was efficacious. Thus, this study confirms that risk communication can be implemented as a primary prevention tool in welding apprenticeships.

  9. Genotypic and antimicrobial characterization of pathogenic bacteria at different stages of cattle slaughtering in southern Brazil.

    PubMed

    Loiko, Márcia R; de Paula, Cheila M D; Langone, Ana C J; Rodrigues, Rochele Q; Cibulski, Samuel; Rodrigues, Rogério de O; Camargo, Anderson C; Nero, Luís A; Mayer, Fabiana Q; Tondo, Eduardo C

    2016-06-01

    Meat can be contaminated in different stages of the slaughtering process and the identification of these stages is the starting point to implement adequate control measures. The objectives of this study were to assess the presence of pathogenic microorganisms in cattle carcasses, to identify the most important contamination points of the slaughtering process, and to evaluate the possible risk factors related to them in a cattle slaughterhouse. To this aim, 108 cattle carcasses were sampled at three stages of the slaughtering process: Point 1 (hides after bleeding); Point 2 (carcasses after hide removal); and Point 3 (carcasses immediately after division). Escherichia coli O157:H7, Listeria monocytogenes and Salmonella Livingstone were isolated from the carcasses. Phenotypic and genotypic characterization indicated that there was cross-contamination among animals, since bacteria with identical genotypic and phenotypic profiles were isolated from different animals at the same sampling day. Furthermore, this is the first report about the isolation of E. coli O157:H7 in a bovine slaughterhouse from southern Brazil.

  10. Frequency of thermophilic Campylobacter in broiler chickens during industrial processing in a Southern Brazil slaughterhouse.

    PubMed

    Franchin, P R; Ogliari, P J; Batista, C R V

    2007-04-01

    1. The frequency of thermophilic Campylobacter spp. on broiler carcases was determined during processing in a Southern Brazil slaughterhouse. Samples were collected after defeathering, evisceration, water chilling and freezing. In addition, samples were obtained from the water of the chiller tank and from the surface of equipment in direct contact with the chicken. 2. Samples (335) were analysed and 71.3% were positive for Campylobacter. The frequency of Campylobacter spp. on carcases rinsed in BPW and skin samples from carcases was 49 of 72 (68.0%) after defeathering, 50 of 72 (69.4%) after evisceration, 61 of 72 (84.7%) after chilling, and 46 of 72 (63.9%) after freezing. Campylobacter was positive for 21 of 23 (91.3%) samples in the chilling water and for 12 of 24 (50.0%) samples on the table surface. 3. The frequency of qualitative analysis for Campylobacter spp. was reduced in frozen chickens, but not during the slaughtering process. The use of drinking water alone as a decontaminant to reduce the incidence of Campylobacter spp. during slaughter is therefore not sufficient. Furthermore, to ensure food safety, chickens must be cooked properly before consuming.

  11. Microbial, physical and chemical properties of irrigation water in rice fields of Southern Brazil.

    PubMed

    Reche, Maria Helena L R; Machado, Vilmar; Saul, Danilo A; Macedo, Vera R M; Marcolin, Elio; Knaak, Neiva; Fiuza, Lidia M

    2016-03-01

    This paper presents the results of the statistical analysis of microbiological, physical and chemical parameters related to the quality of the water used in rice fields in Southern Brazil. Data were collected during three consecutive crop years, within structure of a comprehensive monitoring program. The indicators used were: potential hydrogen, electrical conductivity, turbidity, nitrogen, phosphorus, potassium, calcium, total and fecal coliforms. Principal Component and Discriminant Analysis showed consistent differences between the water irrigation and drainage, as the temporal variation demonstrated a clear reduction in the concentration of most of the variables analyzed. The pattern of this reduction is not the same in the two regions - that is, the importance of each of the different variables in the observed differentiation is modified in two locations. These results suggested that the variations in the water quality utilized for rice irrigation was influenced by certain specific aspects of each rice region in South Brazilian - such as anthropic action or soil/climate conditions in each hydrographic basin.

  12. Acanthamoeba T4, T5 and T11 isolated from mineral water bottles in southern Brazil.

    PubMed

    Maschio, Vinicius José; Chies, Fernanda; Carlesso, Ana Maris; Carvalho, Amanda; Rosa, Sayonara Peixoto; Van Der Sand, Sueli Teresinha; Rott, Marilise Brittes

    2015-01-01

    Acanthamoeba is a protist potential pathogen, capable of causing a blinding keratitis in contact lens wearers and disseminated infection, leading to granulomatous amebic encephalitis in immunocompromised individuals. This amoeba is a ubiquitous organism that has been isolated from various domestic water systems, such as cooling towers and hospital water networks. The objective of this work was to investigate the presence of Acanthamoeba in mineral water bottles marketed in Porto Alegre, southern Brazil. Positive samples were further classified at the genotype level after sequencing the ASA.S1 region of 18S rDNA gene. Six of the eight isolates belonged to T5 genotype, one to T4 genotype, and one was T11. Several genotypes have been reported worldwide as causative of pathologies in humans, including genotypes T4, T5 and T11. Overall, the widespread distribution of potentially pathogenic Acanthamoeba strains in the studied source demands more awareness within the public and health professionals, because this pathogen is emerging as a risk for human health worldwide.

  13. Genetic structure of drone congregation areas of Africanized honeybees in southern Brazil.

    PubMed

    Collet, Thais; Cristino, Alexandre Santos; Quiroga, Carlos Fernando Prada; Soares, Ademilson Espencer Egea; Del Lama, Marco Antônio

    2009-10-01

    As yet, certain aspects of the Africanization process are not well understood, for example, the reproductive behavior of African and European honeybees and how the first Africanized swarms were formed and spread. Drone congregation areas (DCAs) are the ideal place to study honeybee reproduction under natural conditions since hundreds of drones from various colonies gather together in the same geographical area for mating. In the present study, we assessed the genetic structure of seven drone congregations and four commercial European-derived and Africanized apiaries in southern Brazil, employing seven microsatellite loci for this purpose. We also estimated the number of mother-colonies that drones of a specific DCA originated from. Pairwise comparison failed to reveal any population sub-structuring among the DCAs, thus indicating low mutual genetic differentiation. We also observed high genetic similarity between colonies of commercial apiaries and DCAs, besides a slight contribution from a European-derived apiary to a DCA formed nearby. Africanized DCAs seem to have a somewhat different genetic structure when compared to the European.

  14. A new species of Brachycephalus (Anura: Brachycephalidae) from Santa Catarina, southern Brazil.

    PubMed

    Bornschein, Marcos R; Ribeiro, Luiz F; Blackburn, David C; Stanley, Edward L; Pie, Marcio R

    2016-01-01

    A new species of Brachycephalus (Anura: Brachycephalidae) is described from the Atlantic Forest of northeastern state of Santa Catarina, southern Brazil. Nine specimens (eight adults and a juvenile) were collected from the leaf litter of montane forests 790-835 m above sea level (a.s.l.). The new species is a member of the pernix group by its bufoniform shape and the absence of dermal co-ossification and is distinguished from all its congeners by a combination of its general coloration (dorsal region of head, dorsum, legs, arms, and flanks light, brownish green to dark, olive green, with darker region in the middle of the dorsum and a white line along the vertebral column in most specimens) and by its smooth dorsum. The geographical distribution of the new species is highly reduced (extent of occurrence estimated as 25.04 ha, or possibly 34.37 ha). In addition, its habitat has experienced some level of degradation, raising concerns about the future conservation of the species. Preliminary density estimates suggest one calling individual every 3-4 m(2) at 815-835 m a.s.l. and every 100 m(2) at 790 m a.s.l. Together with the recently described B. boticario and B. fuscolineatus, the new species is among the southernmost species of Brachycephalus known to date.

  15. Social vulnerability and climate variability in southern Brazil: a TerraPop case study

    NASA Astrophysics Data System (ADS)

    Adamo, S. B.; Fitch, C. A.; Kugler, T.; Doxsey-Whitfield, E.

    2014-12-01

    Climate variability is an inherent characteristic of the Earth's climate, including but not limited to climate change. It affects and impacts human society in different ways, depending on the underlying socioeconomic vulnerability of specific places, social groups, households and individuals. This differential vulnerability presents spatial and temporal variations, and is rooted in historical patterns of development and relations between human and ecological systems. This study aims to assess the impact of climate variability on livelihoods and well-being, as well as their changes over time and across space, and for rural and urban populations. The geographic focus is Southern Brazil-the states of Parana, Santa Catarina and Rio Grande do Sul-- and the objectives include (a) to identify and map critical areas or hotspots of exposure to climate variability (temperature and precipitation), and (b) to identify internal variation or differential vulnerability within these areas and its evolution over time (1980-2010), using newly available integrated data from the Terra Populus project. These data include geo-referenced climate and agricultural data, and data describing demographic and socioeconomic characteristics of individuals, households and places.

  16. Risk Perception and Occupational Accidents: A Study of Gas Station Workers in Southern Brazil

    PubMed Central

    Cezar-Vaz, Marta Regina; Rocha, Laurelize Pereira; Bonow, Clarice Alves; da Silva, Mara Regina Santos; Vaz, Joana Cezar; Cardoso, Letícia Silveira

    2012-01-01

    The present study aimed to identify the perceptions of gas station workers about physical, chemical, biological and physiological risk factors to which they are exposed in their work environment; identify types of occupational accidents involving gas station workers and; report the development of a socioenvironmental intervention as a tool for risk communication to gas station workers. A quantitative study was performed with 221 gas station workers in southern Brazil between October and December 2010. Data collection was performed between October to December 2010 via structured interviews. The data were analyzed using SPSS 19.0. The participants identified the following risk types: chemical (93.7%), physical (88.2%), physiological (64.3%) and biological (62.4%). In this sample, 94.1% of gas station workers reported occupational accidents, and 74.2% reported fuel contact with the eyes (p < 0.05). It is concluded that workers perceive risks, and that they tend to relate risks with the occurrence of occupational accidents as an indicator of the dangerous nature of their work environment. PMID:22851948

  17. [Factors associated with low levels of lumbar strength in adolescents in Southern Brazil].

    PubMed

    Silva, Diego Augusto Santos; Gonçalves, Eliane Cristina de Andrade; Grigollo, Leoberto Ricardo; Petroski, Edio Luiz

    2014-12-01

    To determine the prevalence and factors associated with low levels of lumbar strength in adolescents. This was a cross-sectional study involving 601 adolescents, aged 14 to 17 years, enrolled in public schools in the western region of Santa Catarina State - Southern Brazil. Lumbar strength was analyzed by the lumbar extension test developed by the Canadian Society of Exercise Physiology, which proposes different cutoffs for boys and girls. Independent variables were sex, age, socioeconomic status, dietary habits, alcohol consumption, physical activity, and aerobic fitness. For data analysis, univariate and multivariate logistic regression were used, with significance level of 5%. The prevalence of low levels of lumbar strength was 27.3%. The population subgroups most likely to present low levels of lumbar strength were females (OR: 1.54, 95% CI: 1.06 to 2.23), adolescents with low levels of aerobic fitness (OR: 2.10, 95% CI: 1.41 to 3.11) and the overweight (OR: 2.28, 95% CI: 1.35 to 3.81). Almost one-third of the studied students have low levels of lumbar strength. Interventions in the school population should be taken with special attention to female adolescents, those with low levels of aerobic fitness, and those with overweight, as these population subgroups were most likely to demonstrate low levels of lumbar strength. Copyright © 2014 Associação de Pediatria de São Paulo. Publicado por Elsevier Editora Ltda. All rights reserved.

  18. Geology and impact features of Vargeão Dome, southern Brazil

    NASA Astrophysics Data System (ADS)

    Crã`Sta, Alvaro P.; Kazzuo-Vieira, César; Pitarello, Lidia; Koeberl, Christian; Kenkmann, Thomas

    2012-01-01

    Vargeão Dome (southern Brazil) is a circular feature formed in lava flows of the Lower Cretaceous Serra Geral Formation and in sandstones of the Paraná Basin. Even though its impact origin was already proposed in the 1980s, little information about its geological and impact features is available in the literature. The structure has a rim-rim diameter of approximately 12 km and comprises several ring-like concentric features with multiple concentric lineaments. The presence of a central uplift is suggested by the occurrence of deformed sandstone strata of the Botucatu and Pirambóia formations. We present the morphological/structural characteristics of Vargeão Dome, characterize the different rock types that occur in its interior, mainly brecciated volcanic rocks (BVR) of the Serra Geral Formation, and discuss the deformation and shock features in the volcanic rocks and in sandstones. These features comprise shatter cones in sandstone and basalt, as well as planar microstructures in quartz. A geochemical comparison of the target rock equivalents from outside the structure with the shocked rocks from its interior shows that both the BVRs and the brecciated sandstone have a composition largely similar to that of the corresponding unshocked lithologies. No traces of meteoritic material have been found so far. The results confirm the impact origin of Vargeão Dome, making it one of the largest among the rare impact craters in basaltic targets known on Earth.

  19. Prevalence of ischemic heart disease and associated factors in patients with rheumatoid arthritis in Southern Brazil.

    PubMed

    Gomes, Rafael Kmiliauskis Santos; Albers, Ana Carolina; Salussoglia, Ana Isadora Pianowski; Bazzan, Ana Maria; Schreiner, Luana Cristina; Vieira, Mateus Oliveira; Silva, Patrícia Giovana da; Machado, Patrícia Helena; Silva, Cynthia Mara da; Mattos, Mauro Marcelo; Nobre, Moacyr Roberto Cuce

    2016-12-21

    To estimate the prevalence of ischemic heart disease and associated factors in patients with rheumatoid arthritis. A cross-sectional study using the American College of Rheumatology diagnostic criteria in order to select patients seen at primary or secondary health care units in Blumenau, Santa Catarina, Southern Brazil, in 2014. The presence of ischemic heart disease was defined as an acute myocardial infarction with percutaneous coronary intervention or coronary artery bypass graft surgery that has occurred after diagnosis. Fischer's exact test, Wald's linear trend test, and multivariate logistic regression analysis were used to test the associations. Among 296 patients (83.1% female) with a mean age of 56.6 years and a mean rheumatoid arthritis duration of 11.3 years, 13 reported having acute myocardial infarction requiring a percutaneous or surgical reperfusion procedure, a prevalence of 4.4% (95% CI 2.0-6.7). Diabetes Mellitus (odds ratio [OR] 4.9 (95% CI 1.6-13.8) and disease duration >10 years (OR 8.2 [95% CI 1.8-39.7]) were the only factors associated with an ischemic disease that remained in the final model, after the multivariate analysis. The prevalence of acute myocardial infarction was similar to that observed in other studies. Among the traditional risk factors, Diabetes Mellitus, and among the factors related to rheumatoid arthritis, disease duration, were the variables associated with comorbidity. Copyright © 2016. Published by Elsevier Editora Ltda.

  20. Risk Communication Concerning Welding Fumes for the Primary Preventive Care of Welding Apprentices in Southern Brazil

    PubMed Central

    Cezar-Vaz, Marta Regina; Bonow, Clarice Alves; Cezar Vaz, Joana

    2015-01-01

    This study’s aim was to assess the perceptions of welding apprentices concerning welding fumes being associated with respiratory and cardiovascular disorders and assess the implementation of risk communication as a primary prevention tool in the welding training process. This quasi-experimental, non-randomized study with before-and-after design was conducted with 84 welding apprentices in Southern Brazil. Poisson Regression analysis was used. Relative Risk was the measure used with a 95% confidence interval and 5% (p ≤ 0.05) significance level. Significant association was found between perceptions of worsened symptoms of respiratory disorders caused by welding fumes and educational level (p = 0.049), the use of goggles to protect against ultraviolet rays (p = 0.023), and access to services in private health facilities without insurance coverage (p = 0.001). Apprentices younger than 25 years old were 4.9 times more likely to perceive worsened cardiovascular symptoms caused by welding fumes after risk communication (RR = 4.91; CI 95%: 1.09 to 22.2). The conclusion is that risk communication as a primary preventive measure in continuing education processes implemented among apprentices, who are future welders, was efficacious. Thus, this study confirms that risk communication can be implemented as a primary prevention tool in welding apprenticeships. PMID:25607606

  1. Influence of habitat structure on fish assemblage of an artificial reef in southern Brazil.

    PubMed

    Hackradt, Carlos Werner; Félix-Hackradt, Fabiana Cézar; García-Charton, José Antonio

    2011-12-01

    Habitat complexity strongly influences reef fish community composition. An understanding of the underlying reasons for this relationship is important for evaluating the suitability of artificial reef (AR) habitats as a marine resource management tool. We studied the influence of AR habitat structure on fish assemblage composition off the southern coast of Brazil. We found that reef blocks with greater area and number of holes possessed the greatest fish species richness and abundance. Reef blocks with greater complexity had higher abundance of almost 30% of fish species present. Natural reef (NR) and AR were different in their fish species composition, trophic structure and categories of water column occupancy by fish (spatial categories). Although NR was more diverse and harboured more trophic levels, AR presented the higher abundances and the presence of distinct fish species that underlined their importance at a regional scale. The greater availability of sheltering habitat where hard substrate is scarce, together with their frequent use by economically important species, make AR a useful tool for coastal management when certain ecological conditions are met. Copyright © 2011 Elsevier Ltd. All rights reserved.

  2. Knowledge, attitudes, and practices of women farmers concerning tobacco agriculture in a municipality in Southern Brazil.

    PubMed

    Reis, Marcelo Moreno Dos; Oliveira, Ana Paula Natividade de; Turci, Silvana Rubano Barretto; Dantas, Renato Maciel; Silva, Valéria Dos Santos Pinto da; Gross, Cátia; Jensen, Teresinha; Silva, Vera Luiza da Costa E

    2017-09-21

    The study aimed to explore the knowledge, attitudes, and practices of women farmers working in tobacco production concerning the social, environmental, and health impacts of this economic activity. Focus groups were used in this qualitative study, and themes were explored until reaching saturation. The study was conducted in a municipality (county) in southern Brazil in 2013 and included 64 women farmers. The discussions revealed participants' familiarity with health problems associated with workloads in tobacco production: green tobacco sickness, pesticide poisoning, musculoskeletal disorders, and others. The discussions also revealed a concern with the negative impacts of tobacco agriculture on the environment. They also revealed apprehension concerning decisions on switching to alternatives for sustainable agricultural production, emphasizing that on-going and systematic government support would be necessary for such a transition. Women farmers identified various factors that contribute to the persistence of tobacco farming: small holdings for cultivation, lack of guarantees for marketing crops, and indebtedness to tobacco companies. The study showed that an integrated approach is needed to deal with tobacco farmers' problems, considering a balance between farmers' beliefs and government decisions. This approach, in keeping with the recommendations of the WHO Framework Convention on Tobacco Control, may help strengthen policies and measures to promote health and sustainable local development.

  3. First insight into the molecular epidemiology of Mycobacterium tuberculosis in Santa Catarina, southern Brazil.

    PubMed

    Nogueira, Christiane Lourenço; Prim, Rodrigo Ivan; Senna, Simone Gonçalves; Rovaris, Darcita Büerger; Maurici, Rosemeri; Rossetti, Maria Lúcia; Couvin, David; Rastogi, Nalin; Bazzo, Maria Luiza

    2016-03-01

    Molecular epidemiology of Mycobacterium tuberculosis is useful for understanding disease transmission dynamics, and to establish strategic measures for TB control and prevention. The aim of this study was to analyze clinical, epidemiological and molecular characteristics of MTBC clinical isolates from Santa Catarina state, southern Brazil. During one-year period, 406 clinical isolates of MTBC were collected from Central Laboratory of Public Health and typed by spoligotyping. Demographic and clinical data were collected from the Brazilian National Mandatory Disease Reporting System. The majority of cases occurred in highest population densities regions and about 50% had some condition associated with TB. Among all isolates, 5.7% were MDR, which showed association with drug addiction. LAM was the most predominant lineage with 47.5%, followed by the T superfamily with 25.9% and Haarlem with 12.3%. The MST showed two major groups: the first was formed mainly by the LAM lineage and the second was mainly formed by the T and Haarlem lineages. Others lineages were distributed in peripheral positions. This study provides the first insight into the population structure of M. tuberculosis in SC State. Spoligotyping and other genotyping analyses are important to establish strategic measures for TB control and prevention. Copyright © 2015 Elsevier Ltd. All rights reserved.

  4. Description of the karyotype of Rhagomys rufescens Thomas, 1886 (Rodentia, Sigmodontinae) from Southern Brazil Atlantic forest

    PubMed Central

    2010-01-01

    Rhagomys rufescens (Rodentia: Sigmodontinae) is an endemic species of the Atlantic forest from Southern and Southeastern Brazil. Some authors consider Rhagomys as part of the tribe Thomasomyini; but its phylogenetic relationships remain unclear. Chromosomal studies on eight specimens of Rhagomys rufescens revealed a diploid number of 2n = 36 and a number of autosome arms FN = 50. GTG, CBG and Ag-NOR banding and CMA3 /DAPI staining were performed on metaphase chromosomes. Eight biarmed and nine acrocentric pairs were found in the karyotype of this species. The X and Y chromosomes were both acrocentric. Most of the autosomes and the sex chromosomes showed positive C-bands in the pericentromeric region. The X chromosome showed an additional heterochromatic block in the proximal region of the long arm. Nucleolus organizer regions (NORs) were located in the pericentromeric region of three biarmed autosomes (pairs 4, 6 and 8) and in the telomeric region of the short arm of three acrocentrics (pairs 10, 12 and 17). CMA 3 /DAPI staining produced fluorescent signals in many autosomes, especially in pairs 4, 6, and 8. This study presents cytogenetic data of Rhagomys rufescens for the first time. PMID:21637420

  5. Occurrence and phenotypic and molecular characterization of Listeriamonocytogenes and Salmonella spp. in slaughterhouses in southern Brazil.

    PubMed

    Iglesias, Mariana Almeida; Kroning, Isabela Schneid; Decol, Luana Tombini; de Melo Franco, Bernadette Dora Gombossy; Silva, Wladimir Padilha da

    2017-10-01

    This study addressed the occurrence of Listeriamonocytogenes and Salmonella spp. in bovine carcasses at two slaughterhouses in southern Brazil. Then, the antimicrobial susceptibility profile and the virulence potential of the isolates were evaluated. Two hundred carcasses were sampled at four steps of the slaughter process, with L. monocytogenes being isolated in 12 and Salmonella spp. in 17 carcasses. All L. monocytogenes isolates carried the hlyA, prfA, plcA, plcB, actA, iap, mpl, inlA, inlB, inlC, and inlJ genes, while Salmonella spp. carried invA and hilA. Among the L. monocytogenes isolates, all of them presented virulence determinants and one showed multi-drug resistance. In relationship to Salmonella spp. isolates, many serogroups frequently related to outbreaks of foodborne diseases were identified and four isolates showed resistance to more than one antimicrobial agent. This data highlights the importance of a rigid hygienic-sanitary control during the slaughter process to reduce the risk of cross-contamination and lower the consumer exposure to L. monocytogenes and Salmonella spp. infections. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Frequency of species of the genus Eimeria in naturally infected cattle in Southern Bahia, Northeast Brazil.

    PubMed

    Almeida, Valter Dos Anjos; de Magalhães, Vanessa Carvalho Sampaio; Neta, Elza de Souza Muniz; Munhoz, Alexandre Dias

    2011-01-01

    The aim of this study was to determine the presence of species of the genus Eimeria species in naturally infected bovines in Southern Bahia, Northeast Brazil. The study population comprised 117 Zebu crossbred cattle that belonged to 10 dairy herds with extensive or semi-extensive production systems. The modified Gordon and Whitlock technique was used to determine positive samples and number of oocysts per gram of feces. Statistical analyses were performed using the chi-square test with Yates correction and a 95% confidence interval. Thirty-nine cattle (33.33%) were positive, and ten different species were identified in infected animals: E. bovis (24.79%); E. canadensis (8.55%); E. zuernii (6.83%); E. ellipsoidalis (5.99%); E. cylindrica (3.42%); E. auburnensis (3.42%); E. brasiliensis (2.56%); E. bukidnonensis (1.71%); E. alabamensis (0.85%), and E. subspherica (0.85%). Higher parasitism was observed in animals up to one year of age (p = 0.005), but no animal presented clinical signs of the disease. As the presence of clinical eimeriosis was not evidenced and all animals were Zebu crossbred cattle from extensive or semi-extensive production systems, further studies should be conducted to investigate the effects of these factors on disease development.

  7. Tropical Rain Forest and Climate Dynamics of the Atlantic Lowland, Southern Brazil, during the Late Quaternary

    NASA Astrophysics Data System (ADS)

    Behling, Hermann; Negrelle, Raquel R. B.

    2001-11-01

    Palynological analysis of a core from the Atlantic rain forest region in Brazil provides unprecedented insight into late Quaternary vegetational and climate dynamics within this southern tropical lowland. The 576-cm-long sediment core is from a former beach-ridge "valley," located 3 km inland from the Atlantic Ocean. Radio-carbon dates suggest that sediment deposition began prior to 35,000 14C yr B.P. Between ca. 37,500 and ca. 27,500 14C yr B.P. and during the last glacial maximum (LGM; ca. 27,500 to ca. 14,500 14C yr B.P.), the coastal rain forest was replaced by grassland and patches of cold-adapted forest. Tropical trees, such as Alchornea, Moraceae/Urticaceae, and Arecaceae, were almost completely absent during the LGM. Furthermore, their distributions were shifted at least 750 km further north, suggesting a cooling between 3°C and 7°C and a strengthening of Antarctic cold fronts during full-glacial times. A depauperate tropical rain forest developed as part of a successional sequence after ca. 12,300 14C yr B.P. There is no evidence that Araucaria trees occurred in the Atlantic lowland during glacial times. The rain forest was disturbed by marine incursions during the early Holocene period until ca. 6100 14C yr B.P., as indicated by the presence of microforaminifera. A closed Atlantic rain forest then developed at the study site.

  8. Mycoflora and deoxynivalenol in whole wheat grains (Triticum aestivum L.) from Southern Brazil.

    PubMed

    Savi, Geovana D; Piacentini, Karim C; Tibola, Casiane S; Scussel, Vildes M

    2014-01-01

    The fungal species Fusarium graminearum is related to deoxynivalenol (DON) formation. The aim of this study was to evaluate mycoflora and DON occurrence in 53 whole wheat grain samples collected in Southern Brazil during the 2012 crop. Wheat grains showed adequate values of water activity ranging from 0.48 to 0.72, within the required limits of moisture content, ranging from 9.1% to 13.9%. In addition, low counts of fungal colonies, ranging from 10 to 8.2 × 10(2), were found. For Fusarium genera, there was predominance of Fusarium verticillioides (34%) and F graminearum (30.2%). For Aspergillus species, 37.7% of Aspergillus flavus was determined. Regarding the Penicillium species, Penicillium digitatum (49%) was the most found species. DON was detected in 47.2% (25 out of 53) of the samples analysed, with levels ranging from 243.7 to 2281.3 µg kg(-1) (mean: 641.9 µg kg(-1)).

  9. Holocene dynamics of vegetation change in southern and southeastern Brazil is consistent with climate forcing

    NASA Astrophysics Data System (ADS)

    Rodrigues, Jackson Martins; Behling, Hermann; Giesecke, Thomas

    2016-08-01

    At mid to high northern latitudes postglacial vegetation change has often occurred synchronously over large regions triggered mainly by abrupt climate change. Based on 19 pollen diagrams from southern and southeastern Brazil we explore if similar synchronicities in vegetation change were also characteristic for the vegetation dynamics in low latitudes. We used sequence splitting to detect past vegetation change in the pollen diagrams and computed principal curves and rates of change to visually evaluate the changes in composition and dynamics. The results show that vegetation change occurred mostly during the second half of the Holocene with distinct episodes of change. The character of vegetation change is generally consistent with shifts to wetter conditions and agrees with inferred shifts of the South American Monsoon. Speleothems as well as the titanium record from the Cariaco Basin indicate several episodes of rapid shifts in the precipitation regime, which are within the dating uncertainty of the here detected periods of vegetation change (8900, 5900, 2800, 1200 and 550 cal yrs BP). Our results indicate that low latitude vegetation composition follows precession forcing of the hydrology, while change is often triggered and synchronized by rapid climate change much like in high and mid latitudes. Pollen diagrams document changes in the abundance of individual taxa and changes in the amount of woodland cover, while small compositional changes indicate a regional stability of vegetation types during the Holocene.

  10. Epistylis portoalegrensis n. sp. (Ciliophora, Peritrichia): A New Freshwater Ciliate Species from Southern Brazil.

    PubMed

    Kühner, Steffen; Simão, Taiz L L; Safi, Lúcia S L; Gazulha, Fernanda B; Eizirik, Eduardo; Utz, Laura R P

    2016-01-01

    The peritrich ciliate Epistylis portoalegrensis n. sp. was found in two bodies of freshwater located in Porto Alegre, Southern Brazil. Morphological features were investigated using live and protargol-stained specimens. The zooids presented a vase to cylindrical shape narrowed at the scopula, and a mean size of 131 × 37 μm in vivo. A C-shaped macronucleus lay in the middle of the cell close to a single contractile vacuole. The oral infraciliature was typical for the genus, with all infundibular polykineties composed by three distinct rows of kinetosomes. Colonies are often nonbranched with no lateral stalk, carrying several zooids stemming from a single point. Specimens from the two sampling sites showed identical arrangement of the infraciliature, similar morphometry, identical 18S rDNA sequences, and a single nucleotide difference across the more variable ITS regions. Molecular phylogenetic analyses placed E. portoalegrensis in a well-supported clade containing other Epistylis species, within the order Vorticellida. © 2015 The Author(s) Journal of Eukaryotic Microbiology © 2015 International Society of Protistologists.

  11. Prevalence of hearing problems and associated factors in an agricultural company in southern Brazil.

    PubMed

    Haeffner, Rafael; Sarquis, Leila Maria Mansano; Heck, Rita Maria; Jardim, Vanda Maria da Rosa

    2015-01-01

    To describe the prevalence and to analyze the factors associated with hearing problems in an agricultural company. This was a cross-sectional study, and the participants consisted entirely of employees of an agricultural company in southern Brazil. The workforce of the company was composed of several different occupations. The research instrument was a semi-structured questionnaire administered by interview. Of the 326 workers of the company, there were 273 (83.8%) respondents, and the prevalence of hearing problems was n = 42 (15.4%) among the participants of the study. The hearing problems were associated with lower schooling, with the operating occupational group, the technical assistance group and the general services group. The self-reported health conditions associated with hearing problems were depression and nervousness or irritation. The occupational exposures associated with hearing problems were noise, dust, vibration, oils and solvents, and toxic gases. There was an intermediate prevalence of hearing problems in relation to other studies. It was possible to observe the need to implement strategies aiming at the prevention of hearing problems that primarily contemplate modifiable aspects, such as the use of personal protective equipment, as well as improvements in the conditions, organization and work environment.

  12. [Nutritional status and social determinants of child height in the Guarita Indigenous Territory, Southern Brazil].

    PubMed

    Menegolla, Ivone Andreatta; Drachler, Maria de Lourdes; Rodrigues, Inajara Haubert; Schwingel, Lucio Roberto; Scapinello, Elaine; Pedroso, Maisa Beltrame; Leite, José Carlos de Carvalho

    2006-02-01

    The nutritional status of under-five children and the association between social conditions and child stature were examined using data from the program to control malnutrition and mortality in the Guarita Indigenous Territory, southern Brazil, 2001-2002. Anthropometric indices were calculated in z-scores of the CDC 2000 reference. At entrance into the program, 34.7% of the children presented stunting, 12.9% low weight for age, 4.2% wasting, and 8.7% overweight. Stunting was most prevalent among boys and children older than one year. Multivariate linear regression showed that, on average, children were shorter when the drinking water was collected directly in the environment (p = 0.046), there was no refrigerator for food preservation (p = 0.021), maternal age was less than 16 years at the birth of the oldest child among the under-fives (p = 0.019), and the mother was illiterate (p = 0.083). Sewage facilities only had an effect on the unadjusted model. There was no evidence that the number of under-five children had an effect on stature. Social inclusion policies and health and social provision which takes these factors into account are potentially relevant for improving health and nutrition in this population.

  13. [Equity and provision of public dental services in the State of Paraná, Southern Brazil].

    PubMed

    Baldani, Márcia Helena; Almeida, Eurivaldo Sampaio de; Antunes, José Leopoldo Ferreira

    2009-06-01

    To assess the association between socioeconomic indicators of provision of public dental services and allocation of financial resources in health and to identify whether this association reinforced the promotion of vertical equity. A cross-sectional ecological study was conducted based on data obtained from the Brazilian Ministry of Health data system for 399 cities in the State of Paraná (Southern Brazil) between 1998 and 2005. Socioeconomic condition was measured by the Human Development Index in the cities studied, as well as by some indicators of income, education and sanitation, which were obtained from the Brazilian Institute of Geography and Statistics database. Data were analyzed using nonparametric statistical tests: Spearman's rank correlation coefficient, Friedman and Mann-Whitney tests. A redistribution trend of federal resources for primary health care in municipalities was seen. The provision of dental health services increased after the government launched the Family Health Strategy. An expanded provision of dental procedures was reported during the study period with a pro-equity trend of the utilization of dental services in primary health care. There was seen a redistribution or pro-equity trend in the provision of dental services in the state of Paraná with higher per capita provision of resources or services in cities with the lowest socioeconomic indicators. This trend is consistent with the recent programmatic guidelines of the Brazilian Ministry of Health.

  14. A new species of Brachycephalus (Anura: Brachycephalidae) from Santa Catarina, southern Brazil

    PubMed Central

    Bornschein, Marcos R.; Ribeiro, Luiz F.; Blackburn, David C.; Stanley, Edward L.

    2016-01-01

    A new species of Brachycephalus (Anura: Brachycephalidae) is described from the Atlantic Forest of northeastern state of Santa Catarina, southern Brazil. Nine specimens (eight adults and a juvenile) were collected from the leaf litter of montane forests 790–835 m above sea level (a.s.l.). The new species is a member of the pernix group by its bufoniform shape and the absence of dermal co-ossification and is distinguished from all its congeners by a combination of its general coloration (dorsal region of head, dorsum, legs, arms, and flanks light, brownish green to dark, olive green, with darker region in the middle of the dorsum and a white line along the vertebral column in most specimens) and by its smooth dorsum. The geographical distribution of the new species is highly reduced (extent of occurrence estimated as 25.04 ha, or possibly 34.37 ha). In addition, its habitat has experienced some level of degradation, raising concerns about the future conservation of the species. Preliminary density estimates suggest one calling individual every 3–4 m2 at 815–835 m a.s.l. and every 100 m2 at 790 m a.s.l. Together with the recently described B. boticario and B. fuscolineatus, the new species is among the southernmost species of Brachycephalus known to date. PMID:27812425

  15. Characteristics of whistles from resident bottlenose dolphins (Tursiops truncatus) in southern Brazil.

    PubMed

    Azevedo, Alexandre F; Oliveira, Alvaro M; Dalla Rosa, L; Lailson-Brito, J

    2007-05-01

    Little is known of the whistles produced by bottlenose dolphins in the South Atlantic Ocean. A total of 788 whistles were recorded from free-ranging bottlenose dolphins in Patos Lagoon estuary, southern Brazil. The mean number of whistles emitted per minute per animal was 0.8. Bottlenose dolphins emitted a varied repertoire of whistles, in which those with more than one inflection point were the most frequent and there was no predominance of ascending or descending whistles. Whistles recorded had a great frequency range, between 1.2 and 22.3 kHz. Whistle duration was 553.3 (+/- 393.9 ms) and 66.6% of the whistles lasted < 800 ms. Differences in the mean values of the whistles' characters were found between this study and other values previously reported for Tursiops. Bottlenose dolphins in the Patos Lagoon estuary emitted repeated whistle contours and individuals may be sharing some whistle types, as it has been suggested for Tursiops.

  16. Lambs are an important source of atypical enteropathogenic Escherichia coli in southern Brazil.

    PubMed

    Martins, Fernando H; Guth, Beatriz E C; Piazza, Roxane M F; Elias, Waldir P; Leão, Sylvia C; Marzoa, Juan; Dahbi, Ghizlane; Mora, Azucena; Blanco, Miguel; Blanco, Jorge; Pelayo, Jacinta S

    2016-11-30

    Food-producing animals can harbor Escherichia coli strains with potential to cause diseases in humans. In this study, the presence of enteropathogenic E. coli (EPEC) was investigated in fecal samples from 130 healthy sheep (92 lambs and 38 adults) raised for meat in southern Brazil. EPEC was detected in 19.2% of the sheep examined, but only lambs were found to be positive. A total of 25 isolates was characterized and designated atypical EPEC (aEPEC) as tested negative for bfpA gene and BFP production. The presence of virulence markers linked to human disease as ehxA, paa, and lpfAO113 was observed in 60%, 24%, and 88% of the isolates, respectively. Of the 11 serotypes identified, eight were described among human pathogenic strains, while three (O1:H8, O11:H21 and O125:H19) were not previously detected in aEPEC. Associations between intimin subtypes and phylogroups were observed, including eae-θ2/A, eae-β1/B1, eae-α2/B2 and eae-γ1/D. Although PFGE typing of 16 aEPEC isolates resulted in 14 unique pulsetypes suggesting a genetic diversity, specific clones were found to be distributed in some flocks. In conclusion, potentially pathogenic aEPEC strains are present in sheep raised for meat, particularly in lambs, which can better contribute to dissemination of these bacteria than adult animals.

  17. Paleoproterozoic felsic volcanism of the Tapajós Mineral Province, Southern Amazon Craton, Brazil

    NASA Astrophysics Data System (ADS)

    Roverato, M.; Giordano, D.; Echeverri-Misas, C. M.; Juliani, C.

    2016-01-01

    Amazonian rocks record one amongst the most complete and best-preserved Paleoproterozoic magmatic episodes on Earth. The present contribution documents the extremely well preserved paleoproterozoic architecture of a series of felsic rocks found in the Tapajós Mineral Province (TMP), located in the western part of Pará State, southern Amazon Craton, north of Brazil. These rocks are the first to be investigated to comprehend, based on their textural evidences, their emplacement mechanisms. Textural characterization allowed to identify three main facies with, as following reported, 1) chaotic ("Breccia") group, 2) eutaxitic ("Eutax") group and 3) parataxitic ("Paratax") group vitrophyric textures. Given the superb preservation of our samples, the investigated rocks are grouped, according to their grade of welding, into a wide variety of lithofacies from very low-grade to high-grade and rheomorphic ignimbrites. In the "Paratax group" strong similarities with banding in lava flows are observed. Based on the presented data we discuss the effusive or explosive origin of the observed flow mechanisms.

  18. Hydrogeochemistry and statistical analysis applied to understand fluoride provenance in the Guarani Aquifer System, Southern Brazil.

    PubMed

    Marimon, Maria Paula C; Roisenberg, Ari; Suhogusoff, Alexandra V; Viero, Antonio Pedro

    2013-06-01

    High fluoride concentrations (up to 11 mg/L) have been reported in the groundwater of the Guarani Aquifer System (Santa Maria Formation) in the central region of the state of Rio Grande do Sul, Southern Brazil. In this area, dental fluorosis is an endemic disease. This paper presents the geochemical data and the combination of statistical analysis (Principal components and cluster analyses) and geochemical modeling to achieve the hydrogeochemistry of the groundwater and discusses the possible fluoride origin. The groundwater from the Santa Maria Formation is comprised of four different geochemical groups. The first group corresponds to a sodium chloride groundwater which evolves to sodium bicarbonate, the second one, both containing fluoride anomalies. The third group is represented by calcium bicarbonate groundwater, and in the fourth, magnesium is the distinctive parameter. The statistical and geochemical analyses supported by isotopic measurements indicated that groundwater may have originated from mixtures of deeper aquifers and the fluoride concentrations could be derived from rock/water interactions (e.g., desorption from clay minerals).

  19. High frequency of bovine viral diarrhea virus type 2 in Southern Brazil.

    PubMed

    Weber, M N; Silveira, S; Machado, G; Groff, F H S; Mósena, A C S; Budaszewski, R F; Dupont, P M; Corbellini, L G; Canal, C W

    2014-10-13

    Ruminant pestiviruses can infect cattle populations worldwide and cause significant economic losses due to their impact on productivity and health. Knowledge of pestivirus diversity is important for control programs and vaccine development and for determining probable sources of infection. In this paper, we describe a search for ruminant pestiviruses with RT-PCR in sera of 9078 calves from 6 to 12 months of age. The calves were first analyzed in pools and then analyzed individually. Thirty-three RT-PCR positive animals were detected (0.36%) from 6.9% (24) of the 346 herds. The sequencing analysis of the 5' non-coding region and N terminal autoprotease showed the presence of BVDV-1a (15 isolates), -1b (3), -1d (1) and -2b (14), with a higher frequency (42.4%) of BVDV-2 in comparison with other countries. The presence of sheep was significantly associated with BVDV infection. Our results also suggested that a BVDV control program based only on the investigation of cattle would not be successful, especially in regions with farms harboring multiple animal species. This study may also serve as a reference for future control programs in Southern Brazil because it reports the prevalence of cattle with active infections and the genetic background of the circulating strains.

  20. The Association of Health and Income in the Elderly: Experience from a Southern State of Brazil

    PubMed Central

    Fillenbaum, Gerda G.; Blay, Sergio L.; Pieper, Carl F.; King, Katherine E.; Andreoli, Sergio B.; Gastal, Fábio L.

    2013-01-01

    Objectives In high income, developed countries, health status tends to improve as income increases, but primarily through the 50th-66th percentile of income. It is unclear whether the same limitation holds in middle income countries, and for both general assessments of health and specific conditions. Methods Data were obtained from Brazil, a middle income country. In-person interviews with a representative sample of community residents age ≥60 (N=6963), in the southern state of Rio Grande do Sul, obtained information on demographic characteristics including household income and number of persons supported, general health status (self-rated health, functional status), depression, and seven physician-diagnosed, self-reported health conditions. Analyses used household income (adjusted for number supported and economies of scale) together with higher order income terms, and controlled for demographics and comorbidities, to ascertain nonlinearity between income and general and specific health measures. Results In fully controlled analyses income was associated with general measures of health (linearly with self-rated health, nonlinearly with functional status). For specific health measures there was a consistent linear association with depression, pulmonary disorders, renal disorders, and sensory impairment. For musculoskeletal, cardiovascular (negative association), and gastrointestinal disorders this association no longer held when comorbidities were controlled. There was no association with diabetes. Conclusion Contrary to findings in high income countries, the association of household-size-adjusted income with health was generally linear, sometimes negative, and sometimes absent when comorbidities were controlled. PMID:24058505

  1. Southern elephant seal trajectories, fronts and eddies in the Brazil/Malvinas Confluence

    NASA Astrophysics Data System (ADS)

    Campagna, Claudio; Piola, Alberto R.; Rosa Marin, Maria; Lewis, Mirtha; Fernández, Teresita

    2006-12-01

    This study describes the association between transient, mesoscale hydrographic features along the axis of the Brazil-Malvinas Confluence, in the SW Atlantic, and the foraging behavior of 2-3-year-old (focal) juvenile southern elephant seals, Mirounga leonina, from Península Valdés, Argentina. Departing from the dominant pattern of foraging on predictable bathymetric fronts on the Patagonian shelf and slope, three females out of 12 satellite-tracked juveniles remained at the edge of young warm-core eddies and near the outer core of cold-core eddies, coinciding with the most productive areas of these temperature fronts. Seal trajectories along high-temperature gradients were always consistent with the speed and direction of surface currents inferred from the temperature distribution and confirmed by surface drifters. Movements of foraging seals were compared with those of surface drifters, coinciding in time and space and yielding independent and consistent data on regional water circulation parameters. The diving pattern recorded for one focal seal yielded shallower dives and a loose diel pattern in the eddy, and a marked diurnal cycle compatible with foraging on vertically migrating prey in the cold waters of the Malvinas Current. Pre-reproductive females that use the mesoscale fronts of the Argentine Basin as an alternative foraging area would benefit from lower competition with more experienced seals and with other top predators that reproduce along the coast of Patagonia.

  2. The use of appetite suppressants among health sciences undergraduate students in Southern Brazil

    PubMed Central

    Zubaran, Carlos; Lazzaretti, Rubia

    2013-01-01

    ABSTRACT Objective: To investigate the prevalence of appetite suppressant use among health sciences students in Southern Brazil. Methods: Undergraduate students (n=300) from seven health science undergraduate courses of the Universidade de Caxias do Sul completed a questionnaire about the use of substances to suppress appetite. Results: A significant percentage (15%; n=45) of research participants used appetite suppressants at least once in their lives. The most commonly used substances were sympathomimetic stimulant drugs (5%), including amfepramone (3.3%) and fenproporex (1.7%). The lifetime use of appetite suppressants was more prevalent among Nursing (26.7%) and Nutrition (24.4%%) students. There was no reported use of appetite suppressants among medical students. The use of appetite suppressants was significantly more prevalent among women. The majority of those who used these substances did so under medical recommendation. Most of users took appetite suppressants for more than 3 months. Conclusion: Lifetime use of appetite suppressants was substantial, being sympathomimetic stimulant drugs the most commonly used agents. Students enrolled in Nursing and Nutrition courses presented a significantly higher prevalence of lifetime use of appetite suppressants. PMID:23579743

  3. Water and CO2 Exchange for Different Land Use in Pampa Biome in Southern Brazil

    NASA Astrophysics Data System (ADS)

    Roberti, D. R.; de Moraes, O. L. L.; Diaz, M.; Tatsch, J. D.; Acevedo, O. C.; Zimermann, H. R.; Rubert, G. C.; Acosta, R.; Campos Velho, H. F.

    2015-12-01

    The Pampa is the newest and most unknown Brazilian Biome. It is located in the Southern portion of the country, as well as part of Argentina and the entire Uruguay, and is formed principally by natural grasslands that have been used for centuries for grazing livestock. In recent decades it has gone through a process of intense land use change and degradation, with the replacement of natural vegetation by rice paddy crops, soybean and exotic forests. Recent studies show that the Pampa has only 36% of its original vegetation in Brazil. Research on carbon and greenhouse gas emissions in Pampa Biome are recent. It is known that the Pampa natural areas contain high stocks of soil organic carbon, and therefore their conservation is relevant for climate change mitigation. However, the net exchange of carbon and water between the surface and the atmosphere are unknown. To fill this gap, a flux tower network, SULFLUX - www.ufsm.br/sulfux, was created. Currently, SULFLUX comprises three flux towers in the Pampa biome, two of them being over natural vegetation and the other one over a rice paddy. The flux towers are nearly 100 km apart from each other. We examine the effects of climate on carbon fluxes of through the year 2014. Analysis of temporal variability in water and CO2 fluxes are examined at daily to annual scales. Overall, regional variability in climatic drivers, land use and soil proprieties appears to have a greater effect on evapotranspiration than on net carbon exchange.

  4. Differences between renal effects of venom from two Bothrops jararaca populations from southeastern and southern Brazil.

    PubMed

    Jorge, Roberta Jeane Bezerra; Jorge, Antônio Rafael Coelho; de Menezes, Ramon Róseo Paula Pessoa Bezerra; Mello, Clarissa Perdigão; Lima, Danya Bandeira; Silveira, João Alison de Moraes; Alves, Natacha Teresa Queiroz; Marinho, Aline Diogo; Ximenes, Rafael Matos; Corrêa-Netto, Carlos; Gonçalves Machado, Larissa; Zingali, Russolina Benedeta; Martins, Alice Maria Costa; Monteiro, Helena Serra Azul

    2017-01-01

    Components from animal venoms may vary according to the snake's age, gender and region of origin. Recently, we performed a proteomic analysis of Bothrops jararaca venom from southern (BjSv) and southeastern (BjSEv) Brazil, showing differences in the venom composition, as well as its biological activity. To continue the study, we report in this short communication the different effects induced by the BjSEv and BjSv on isolated kidney and MDCK renal cells. BjSEv decreased perfusion pressure (PP) and renal vascular resistance (RVR) and increased urinary flow (UF) and glomerular filtration rate (GFR), while BjSv did not alter PP and RVR and reduced UF and GFR. Both types of venom, more expressively BjSEv, reduced %TNa(+), %TK(+) and %Cl(-). In MDCK cells, the two types of venom showed cytotoxicity with IC50 of 1.22 μg/mL for BjSv and 1.18 μg/mL for BjSEv and caused different profiles of cell death, with BjSv being more necrotic. In conclusion, we suggest that BjSv is more nephrotoxic than BjSEv. Copyright © 2016 Elsevier Ltd. All rights reserved.

  5. Newly discovered reefs in the southern Abrolhos Bank, Brazil: Anthropogenic impacts and urgent conservation needs.

    PubMed

    Mazzei, E F; Bertoncini, A A; Pinheiro, H T; Machado, L F; Vilar, C C; Guabiroba, H C; Costa, T J F; Bueno, L S; Santos, L N; Francini-Filho, R B; Hostim-Silva, M; Joyeux, J-C

    2017-01-15

    The Abrolhos Bank is an area of high ecological, socio-economic importance and harbour the richest and most-extensive coral reefs in the South Atlantic. Here we report the discovery of shallow (12-25m depth) reef complex with ten large biogenic structures, intermediate between the typical mushroom-shaped pinnacles of the northern Abrolhos Bank (17°-18° S) and the small patch reefs found on the central/southern coast of the Espírito Santo State (19°-20° S). The newly discovered reefs harbour a relatively rich and abundant reef community, with 73 fish and 14 benthic cnidarian species, including endangered and commercially important ones. We discuss on urgent needs of properly mapping and understanding the ecological functioning of this reef system. Information provided here is a baseline for future impact evaluations, particularly considering the recent worst environmental disaster of Brazil from a dam collapse in Doce river that affected the region. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Genetic variation of the bronze locus (MC1R) in turkeys from Southern Brazil.

    PubMed

    Corso, Josmael; Hepp, Diego; Ledur, Mônica C; Peixoto, Jane O; Fagundes, Nelson J R; Freitas, Thales R O

    2017-01-01

    Domestic turkeys present several color phenotypes controlled by at least five genetic loci, but only one of these has been identified precisely: the bronze locus, which turned out to be the melanocortin-1 receptor (MC1R) gene. MC1R variation is important for breeders interested in maintaining or developing different color varieties. In this study, we sequenced most of the MC1R gene from 16 White Holland (the main commercial turkey variety) and 19 pigmented turkeys from southern Brazil with two purposes. The first was to describe the MC1R diversity in White Holland turkeys, which may serve as reservoirs of genetic diversity at this locus. The second was to test whether the traditional color classification used by Brazilian breeders is related to previously known MC1R alleles. White Holland turkeys had four different haplotypes corresponding to the bronze (b+) and black-winged bronze (b1) alleles. Pigmented turkeys also had four haplotypes corresponding to the b+ and b1 alleles, but different haplotypes represent the most common b+ allele in these two groups. The black (B) allele was absent from our samples. Overall, our results suggest that white and pigmented individuals form two different populations, and that the traditional color classification used by Brazilian breeders cannot accurately predict the genotypes at the bronze locus.

  7. Genetic variation of the bronze locus (MC1R) in turkeys from Southern Brazil

    PubMed Central

    Corso, Josmael; Hepp, Diego; Ledur, Mônica C.; Peixoto, Jane O.; Fagundes, Nelson J. R.; Freitas, Thales R. O.

    2017-01-01

    Abstract Domestic turkeys present several color phenotypes controlled by at least five genetic loci, but only one of these has been identified precisely: the bronze locus, which turned out to be the melanocortin-1 receptor (MC1R) gene. MC1R variation is important for breeders interested in maintaining or developing different color varieties. In this study, we sequenced most of the MC1R gene from 16 White Holland (the main commercial turkey variety) and 19 pigmented turkeys from southern Brazil with two purposes. The first was to describe the MC1R diversity in White Holland turkeys, which may serve as reservoirs of genetic diversity at this locus. The second was to test whether the traditional color classification used by Brazilian breeders is related to previously known MC1R alleles. White Holland turkeys had four different haplotypes corresponding to the bronze (b +) and black-winged bronze (b 1) alleles. Pigmented turkeys also had four haplotypes corresponding to the b + and b 1 alleles, but different haplotypes represent the most common b + allele in these two groups. The black (B) allele was absent from our samples. Overall, our results suggest that white and pigmented individuals form two different populations, and that the traditional color classification used by Brazilian breeders cannot accurately predict the genotypes at the bronze locus. PMID:28323301

  8. Bolivia-Brazil gas pipeline about to take off; seen as litmus test for Southern Cone gas grid

    SciTech Connect

    1995-08-07

    After more than 4 decades of studies, plans, and shelved projects, the proposed Bolivia-Brazil gas pipeline is finally about to get off the ground. The 3,700 km gas pipeline will require an investment of at least $2 billion and is viewed by many as a litmus test for the developing gas market and energy integration of South America`s Southern Cone countries. Overall, industry officials see eventual emergence of two large integrated gas grids serving South America: one for the northern countries and another for the Southern Cone. This will enable the six countries with gas surplus to their needs to export the surplus to neighboring, gas-short countries. The northern gas-long countries are Venezuela, Colombia, and Trinidad and Tobago; those in the Southern Cone are Argentina, Bolivia, and Peru. The paper discusses financial details, project details, pipeline construction, the Petrobras strategy, Argentine pipeline projects, and other pipeline proposals.

  9. Identification of vector-borne pathogens in dogs and cats from Southern Brazil.

    PubMed

    Malheiros, J; Costa, M M; do Amaral, R B; de Sousa, K C M; André, M R; Machado, R Z; Vieira, M I B

    2016-07-01

    Dogs and cats are often infected with vector-borne pathogens and play a crucial role as reservoirs and hosts in their life cycles. The aim of the present study was to investigate the occurrence of vector-borne pathogens among dogs and cats in the northwestern region of Rio Grande do Sul (RS) State, Brazil. One hundred and ten blood samples were collected from dogs (n=80) and cats (n=30). Laboratory analysis were carried out through stained blood smears, indirect enzyme-linked immunosorbent assay (ELISA) for Babesia vogeli and Ehrlichia canis (only for dogs) and polymerase chain reaction (PCR) aiming the detection of pathogens. The following pathogens were screened by PCR among dogs and cats: Babesia spp. and Hepatozoon spp. (18S rRNA gene), Anaplasma spp. (16S rRNA gene), and Ehrlichia spp. (dsb gene for dogs and 16S rRNA gene for cats) and Bartonella spp. (nuoG gene only for cats). Using blood smears structures morphologically compatible with piroplasms were found in 5.45% (6/110) of the samples. Anti-B. vogeli and anti-E. canis antibodies were detected in 91% (73/80) and 9% (7/80) of the dogs, respectively. All the seropositive dogs to E. canis were also to B. vogeli. Nineteen (17.3%) animals were positive to hemoparasites by PCR. After sequencing Rangelia vitalii 6/80 (7.5%), B. vogeli 3/80 (4%), Hepatozoon spp. 1/80 (1%), and Anaplasma spp. 1/80 (1%) were found in the dogs, and B. vogeli 2/30 (7%) and Bartonella spp. 6/30 (20%) were detected in the screened cats. No sample was positive for genes dsb and 16S rRNA of Ehrlichia spp. Only those animals which were positive for R. vitalii showed findings compatible with rangeliosis, such as anemia (100%), thrombocytopenia (67%), jaundice (50%), external bleeding (50%), and anorexia (50%). This is the first time that B. vogeli detected among cats in Southern Brazil.

  10. Clustering of Risk Factors for Non-Communicable Diseases among Adolescents from Southern Brazil

    PubMed Central

    2016-01-01

    Introduction The aim of this study was to investigate the simultaneous presence of risk factors for non-communicable diseases and the association of these risk factors with demographic and economic factors among adolescents from southern Brazil. Methods The study included 916 students (14–19 years old) enrolled in the 2014 school year at state schools in São José, Santa Catarina, Brazil. Risk factors related to lifestyle (i.e., physical inactivity, excessive alcohol consumption, smoking, sedentary behaviour and unhealthy diet), demographic variables (sex, age and skin colour) and economic variables (school shift and economic level) were assessed through a questionnaire. Simultaneous behaviours were assessed by the ratio between observed and expected prevalences of risk factors for non-communicable diseases. The clustering of risk factors was analysed by multinomial logistic regression. The clusters of risk factors that showed a higher prevalence were analysed by binary logistic regression. Results The clustering of two, three, four, and five risk factors were found in 22.2%, 49.3%, 21.7% and 3.1% of adolescents, respectively. Subgroups that were more likely to have both behaviours of physical inactivity and unhealthy diet simultaneously were mostly composed of girls (OR = 3.03, 95% CI = 1.57–5.85) and those with lower socioeconomic status (OR = 1.83, 95% CI = 1.05–3.21); simultaneous physical inactivity, excessive alcohol consumption, sedentary behaviour and unhealthy diet were mainly observed among older adolescents (OR = 1.49, 95% CI = 1.05–2.12). Subgroups less likely to have both behaviours of sedentary behaviour and unhealthy diet were mostly composed of girls (OR = 0.58, 95% CI = 0.38–0.89); simultaneous physical inactivity, sedentary behaviour and unhealthy diet were mainly observed among older individuals (OR = 0.66, 95% CI = 0.49–0.87) and those of the night shift (OR = 0.59, 95% CI = 0.43–0.82). Conclusion Adolescents had a high prevalence

  11. Synsedimentary deformation and the paleoseismic record in Marinoan cap carbonate of the southern Amazon Craton, Brazil

    NASA Astrophysics Data System (ADS)

    Soares, Joelson Lima; Nogueira, Afonso César Rodrigues; Domingos, Fábio; Riccomini, Claudio

    2013-12-01

    Event Layers in Neoproterozoic cap carbonates of Brazil's southwestern Amazon Craton record post-Marinoan synsedimentary seismicity. The 35 m-thick cap carbonates overlie glaciogenic sediments related to the Marinoan glaciation (635 Ma) and are comprised of two units: the lower cap consists of dolomite (˜15 m thick) and the upper cap is limestone (˜25 m thick). The cap dolomite includes pinkish crystalline dolostone with even parallel lamination, stratiform stromatolites, eventual tube structures and megaripple bedded peloidal dolostone interpreted as shallow (euphotic) platform deposits. The cap limestone onlaps the cap dolomite and consists of red marl, gray to black bituminous lime mudstone, bituminous shale with abundant calcite crystal fans (pseudomorphs after aragonite) and even parallel lamination interpreted as moderately deep to deep platform deposits. Five successive events of synsedimentary deformation were recognized in the cap carbonates exposed at Mirassol d'Oeste and Tangará da Serra, in Central Brazil: Event 1 - large to small-scale load cast structures in the contact between dolostones and glaciogenic sediments; Event 2 - stromatolitic lamination truncated by tube structures; Event 3 - vertical to subvertical fractures and faults, and large-scale synclines and anticlines with chevron folds; Event 4 - conglomerate and breccia filling neptunian dykes limited by undeformed beds; and Event 5 - slump and sliding deposits found only in the upper part of the cap limestone. Event 1 was produced by hydroplastic dynamics likely induced by isostatic rebound during ice cap melting in the final stages of the Marinoan glaciation. Events 2 and 5 are autocyclic in nature, and related to depositional processes. Event 2 is linked to fluid and methane escape from organic degradation of microbial mats and domes that formed tubestones; Event 5 is associated to collapse and sliding/slumping in the platform and slope. The reliable orientations of synsedimentary faults

  12. Tracing anthropogenically driven groundwater discharge into a coastal lagoon from southern Brazil

    NASA Astrophysics Data System (ADS)

    Santos, Isaac R.; Niencheski, Felipe; Burnett, William; Peterson, Richard; Chanton, Jeffrey; Andrade, Carlos F. F.; Milani, Idel B.; Schmidt, Axel; Knoeller, Kay

    2008-05-01

    SummaryWe investigated the distribution of naturally occurring geochemical tracers ( 222Rn, 223Ra, 224Ra, 226Ra, CH 4, δ18O, and δ2H) in the water column and adjacent groundwater of Mangueira Lagoon as proxies of groundwater discharge. Mangueira Lagoon is a large (90 km long), shallow (˜4-5 m deep), fresh, and non-tidal coastal lagoon in southern Brazil surrounded by extensively irrigated rice plantations and numerous irrigation canals. We hypothesized that the annual, intense irrigation for rice agriculture creates extreme conditions that seasonally change groundwater discharge patterns in the adjacent lagoon. We further supposed that dredging of irrigation canals alters groundwater fluxes. While the activities of 222Rn in shallow groundwater were 2-3 orders of magnitude higher than in surface water, CH 4 and radium isotopes were only ˜1 order of magnitude higher. Therefore, 222Rn appears to be the preferred groundwater tracer in this system. Radon concentrations and conductivities were dramatically higher near the pump house of rice irrigation canals, consistent with a groundwater source. Modeling of radon inventories accounting for total inputs (groundwater advection, diffusion from sediments, and decay of 226Ra) and losses (atmospheric evasion, horizontal mixing and decay) indicated that groundwater advection rates in the irrigation canals (˜25 cm/d) are over 2 orders of magnitude higher than along the shoreline (˜0.1 cm/d). Nearly 75% of the total area of the canals is found in the southern half of the lagoon, where groundwater inputs seem to be higher as also indicated by methane and stable isotope trends. In spite of the relatively small area of the canals, we estimate that they contribute nearly 70% of the total (˜57,000 m 3/d) groundwater input into the entire Mangueira Lagoon. We suggest that the dredging of these canals cut through aquitards which previously restricted upward advection from the underlying permeable strata. The irrigation channels

  13. A new aetosaur from the Upper Triassic of the Santa Maria Formation, southern Brazil.

    PubMed

    Roberto-da-Silva, Lúcio; Desojo, Julia B; Cabreira, Sérgio F; Aires, Alex S S; Müller, Rodrigo T; Pacheco, Cristian P; Dias-da-Silva, Sérgio

    2014-02-11

    Aetosaurs are armored pseudosuchian archosaurs widespread in Upper Triassic units. In South America, four taxa were previously recorded: Aetosauroides scagliai, Neoaetosauroides engaeus, Aetobarbakinoides brasiliensis, and Chilenosuchus forttae. Herein we describe a new Late Triassic juvenile aetosaur from the Santa Maria Formation of southern Brazil, Polesinesuchus aurelioi gen. et sp. nov., increasing the paleobiodiversity of this interesting group to five taxa in Western Gondwana. The holotype is composed of cranial (parietal and braincase) and postcranial elements (cervical, dorsal, sacral, caudal vertebrae, both scapulae, a humerus, ilium, pubis, ischium, tibia, a partial right pes, and anterior and mid-dorsal paramedian osteoderms). It belongs to a juvenile individual, as its neurocentral sutures are open in all vertebrae, and also due to its small size. However, future paleohistological investigation is necessary to fully corroborate this assumption. This new taxon is distinguished from all other aetosaurs by the presence of an unique combination of character states (not controlled by ontogeny) such as: cervical vertebrae with prezygapophyses widely extending laterally through most of the anterior edge of the diapophyses; absence of hyposphene articulations in both cervical and mid-dorsal vertebrae; presence of a ventral keel in cervical vertebrae; anterior and mid-dorsal vertebrae without a lateral fossa in their centra; expanded proximal end of scapula; anteroposteriorly expanded medial portion of scapular blade; a short humerus with a robust shaft; and a dorsoventral and very low iliac blade with a long anterior process which slightly exceeds the pubic peduncle. Regarding its phylogenetic relationships, the present analysis placed Polesinesuchus as the sister taxon of Aetobarbakinoides and both as sister taxa of the unnamed monophyletic clade Desmatosuchinae plus Typothoracisinae. 

  14. Resistance against gastrointestinal nematodes in Crioulo Lageano and crossbred Angus cattle in southern Brazil.

    PubMed

    Cardoso, Cristina P; Silva, Bruna F; Trinca, Luzia A; Amarante, Alessandro F T

    2013-02-18

    Gastrointestinal nematode (GIN) infection is a major cause of production losses in cattle. This study was carried out to evaluate the natural resistance against nematode infection in Crioulo Lageano and crossbred Angus male calves. Crioulo Lageano is a local cattle breed in the state of Santa Catarina, in southern Brazil. Ten weaned calves of each breed were grazed together on pasture and naturally infected with nematodes between July 2009 and December 2010. Once every 28 days, we collected fecal and blood samples for parasitological and immunological tests, as well as recording body weights. After 19 samplings, all animals were slaughtered for quantification and identification of GINs. We found that the animals had been infected with the following nematode species, in decreasing order by the mean number of specimens: Trichostrongylus axei, Cooperia punctata, Ostertagia ostertagi, Haemonchus placei, Oesophagostomum radiatum, and Trichuris spp. There were no significant differences between the Crioulo Lageano and crossbred Angus groups in terms of worm burden or nematode fecal egg count, nor in terms of the mean levels of immunoglobulin (G and A) against C. punctata and H. placei antigens, except in IgA mean level in abomasal mucus against H. placei adult worms that was significantly higher in crossbred Angus cattle (p<0.05). At the end of the study, the crossbred Angus cattle were heavier than were the Crioulo Lageano cattle (mean live weight, 507.35 and 390.3 kg, respectively). Comparative parasitological and immunological evaluation revealed no difference between two breeds in terms of their natural resistance against GINs.

  15. Evaluation of two recommended disinfection methods for cleaning cloths used in food services of southern Brazil.

    PubMed

    Bartz, Sabrina; Tondo, Eduardo Cesar

    2013-01-01

    In the State of Rio Grande do Sul (RS), Southern Brazil, a good manufacturing practices regulation was published recommending two disinfection methods for cleaning cloths used in food services. The aim of the present study was to evaluate the efficacy of those methods. Cleaning cloths were sampled without prior notice at food services, on common working days. For the analyses, the cloths were divided in two sub-samples, being one of them microbiologically analyzed. The second sub-sample was further divided in two pieces and submitted to hand washing for two minutes. After that, one piece was boiled in water for 15 min and the other one was soaked in a 200 ppm sodium hypochlorite solution for 15 min. Both pieces of cloth were submitted to microbiological analyses. Cleaning cloths presented total aerobic mean counts of 6.9 ± 6.7 log/cm(2). All cleaning cloths presented coliform contamination, and 40% demonstrated mean counts of 6.2 ± 5.6 log/cm(2). Presumptive S. aureus mean counts of 5.5 ± 4.9 log/cm(2) were found. No statistic correlation was observed among the number of meals served daily in the food services and the microbiological contamination levels. After washing and disinfection, microbiological counts were significantly (p < 0.05) reduced by both methods, achieving an approximately 5 log reduction. The reductions achieved by the sodium hypochlorite soaking method and the boiling method were not significantly different. Thus, it was possible to conclude that both recommended methods were suitable to disinfect cleaning cloths used in food services.

  16. Positive serology for viral hepatitis and donor self-exclusion in Southern Brazil.

    PubMed

    Maccarini, Julia De Luca; Nazario, Carlos Alberto Kuntz; Ferreira, Jovino Dos Santos; Ceglio, William Queiroz Guimarães Wiegandt; Serpa, Rômulo Cavalcante; Ferreira, Vera Lúcia Paes Cavalcanti; Lucca Schiavon, Leonardo de; Narciso-Schiavon, Janaína Luz

    2013-01-01

    Despite the great advances in serological testing for transfusion-transmitted infections, the selection of blood donors by blood bank operators remains the only way to avoid transmission within the testing window period. Part of this selection is the self-exclusion form, on which the donors can exclude their blood from donation without any explanation. This study assessed the clinical and epidemiological characteristics related to positivity for viral hepatitis and to the use of the confidential self-exclusion (CSE) form. This transversal study analyzed the data collected from blood donors' files in a hospital in Southern Brazil. Univariate and multivariate analyses identified the clinical and epidemiological variables related to positive serologies of viral hepatitis and to whether the donor was self-excluded. Of the 3,180 donors included in this study, 0.1% tested positive for HBsAg, 2.1% for anti-HBc, and 0.9% for anti-HCV. When the 93 donors with positive serologies for viral hepatitis were compared with those who were negative, a greater proportion of the positive serology group was found to have had a history of blood transfusions (OR=4.908; 95%CI=1.628 - 14.799; p<0.01), had repeatedly donated (OR=2.147; 95%CI=1.236 - 3.729; p<0.01), and used the CSE form for self-exclusion (OR=7.139; 95%CI=2.045 - 24.923; p<0.01). No variables were independently associated with self-exclusion. A history of blood transfusion, repeated donations, and self-exclusion are factors that should be considered during viral hepatitis screenings in blood banks.

  17. Evaluation of two recommended disinfection methods for cleaning cloths used in food services of southern Brazil

    PubMed Central

    Bartz, Sabrina; Tondo, Eduardo Cesar

    2013-01-01

    In the State of Rio Grande do Sul (RS), Southern Brazil, a good manufacturing practices regulation was published recommending two disinfection methods for cleaning cloths used in food services. The aim of the present study was to evaluate the efficacy of those methods. Cleaning cloths were sampled without prior notice at food services, on common working days. For the analyses, the cloths were divided in two sub-samples, being one of them microbiologically analyzed. The second sub-sample was further divided in two pieces and submitted to hand washing for two minutes. After that, one piece was boiled in water for 15 min and the other one was soaked in a 200 ppm sodium hypochlorite solution for 15 min. Both pieces of cloth were submitted to microbiological analyses. Cleaning cloths presented total aerobic mean counts of 6.9 ± 6.7 log/cm2. All cleaning cloths presented coliform contamination, and 40% demonstrated mean counts of 6.2 ± 5.6 log/cm2. Presumptive S. aureus mean counts of 5.5 ± 4.9 log/cm2 were found. No statistic correlation was observed among the number of meals served daily in the food services and the microbiological contamination levels. After washing and disinfection, microbiological counts were significantly (p < 0.05) reduced by both methods, achieving an approximately 5 log reduction. The reductions achieved by the sodium hypochlorite soaking method and the boiling method were not significantly different. Thus, it was possible to conclude that both recommended methods were suitable to disinfect cleaning cloths used in food services. PMID:24516443

  18. Job characteristics and musculoskeletal pain among shift workers of a poultry processing plant in Southern Brazil.

    PubMed

    Barro, Dânia; Olinto, Maria Teresa Anselmo; Macagnan, Jamile Block Araldi; Henn, Ruth Liane; Pattussi, Marcos Pascoal; Faoro, Mariana Wentz; Garcez, Anderson da Silva; Paniz, Vera Maria Vieira

    2015-01-01

    The purpose of this study was to evaluate the association between job characteristics and musculoskeletal pain among shift workers employed at a 24-hour poultry processing plant in Southern Brazil. This was a cross-sectional study of 1,103 production line workers aged 18-52 years. The job characteristics of interest were shift (day/night), shift duration, and plant sector ambient temperature. Musculoskeletal pain was defined as self-reported occupational-related pain in the upper or lower extremities and trunk, occurring often or always, during the last 12 months. The mean (SD) participant age was 30.8 (8.5) years, and 65.7% of participants were women. The prevalence of musculoskeletal pain was greater among female participants than male participants. After adjustment for job characteristics and potential confounders, the prevalence ratios (PR) of lower extremity musculoskeletal pain among female workers employed in extreme-temperature conditions those working the night shift, and those who had been working longer on the same shift were 1.75 (95% CI 1.12, 2.71), 1.69 (95% CI 1.05, 2.70), and 1.64 (95% CI 1.03, 2.62), respectively. In male workers, only extreme-temperature conditions showed a significant association with lower extremity musculoskeletal pain (PR=2.17; 95% CI 1.12, 4.22) after adjustment analysis. These findings suggest a need for implementation of measures to mitigate the damage caused by nighttime work and by working under extreme temperature conditions, especially among female shift workers, such as changing positions frequently during work and implementation of rest breaks and a workplace exercise program, so as to improve worker quality of life.

  19. Dental caries and associated factors among young male adults between 1999 and 2003 in Southern Brazil.

    PubMed

    Bastos, J L D; Nomura, L H; Peres, M A

    2007-06-01

    To assess the prevalence, severity and distribution of dental caries and associated factors among young male adults from Florianopolis (Southern Brazil) and to compare these results with those from a previous study. A cross-sectional study (n-414) was conducted among Brazilian Army subjects in 2003. Dental caries experience was recorded using the DMF-T Index (WHO, 1997). Non-clinical data were obtained through a questionnaire. The analyses included DMF-T descriptive statistics, calculation of the Gini coefficient to assess inequality in the distribution of caries and non-conditional multiple logistic regression, following a hierarchical approach. The response rate was 95.6%. High values of intra- and inter-examiner agreement were achieved (Kappa > 0.83). Reductions of 18.6% in prevalence and 26.7% in caries severity were accompanied by an increase of 18.6% in dental caries inequality. Individuals with eight or less years of study (OR 8.1 : CI95% 1.9-34.7), raised by mothers with eight or less years of study (OR 2.9 : CI 95% 1.7-5.0) were more likely to have dental caries. Subjects whose families earned less than six Brazilian minimum wages per month were also more likely to have dental caries (OR 2.3 : CI95% 1.4-3.8). Decreases in caries prevalence and severity were followed by an increased inequality in caries distribution. Low level of schooling, low maternal schooling and low monthly family income were statistically associated with dental caries.

  20. Neurocognitive Impairment in HIV-1 Clade C versus B Infected Individuals in Southern Brazil

    PubMed Central

    de Almeida, Sergio Monteiro; Ribeiro, Clea Elisa; de Pereira, Ana Paula; Badiee, Jayraan; Cherner, Mariana; Smith, Davey; Maich, Ingrid; Raboni, Sonia Mara; Rotta, Indianara; Barbosa, Francisco Jaime; Heaton, Robert K.; Umlauf, Anya; Ellis, Ronald J.

    2014-01-01

    HIV-1 clade C isolates show reduced Tat protein chemoattractant activity compared with clade B. This might influence neuropathogenesis by altering trafficking of monocytes into the CNS. A previous study suggested low rates of HIV-associated dementia in clade C infected individuals. The present study evaluated neurocognitive impairment rates in clade B- and C-infected individuals from the same local population. HIV+ and HIV- participants were recruited from the same geographic region in southern Brazil. We evaluated neuropsychological (NP) impairment using a screening instrument (the International HIV Dementia Scale; IHDS), as well as a Brazilian Portuguese adaptation of a comprehensive battery that has demonstrated sensitivity to HIV associated neurocognitive disorders (HAND) internationally. NP performance in controls was used to generate T-scores and impairment ratings by the global deficit score (GDS) method. Clade assignments were ascertained by sequencing pol and env. Blood and cerebrospinal fluid (CSF) were collected from all HIV+ participants. HIV+ and HIV- participants were comparable on demographic characteristics. HIV+ participants overall were more likely to be impaired than HIV- by the IHDS and the GDS. Clade B and C infected individuals were demographically similar and did not differ significantly in rates of impairment. The prevalence of pleocytosis, a marker of intrathecal cellular chemotaxis, also did not differ between clade B and C infections. Clade B and C HIV-infected individuals from the same geographic region, when ascertained using comparable methods, did not differ in their rates of neurocognitive impairment, and there was no evidence of differences in CNS chemotaxis. PMID:24277437

  1. Neurocognitive impairment in HIV-1 clade C- versus B-infected individuals in Southern Brazil.

    PubMed

    de Almeida, Sergio Monteiro; Ribeiro, Clea Elisa; de Pereira, Ana Paula; Badiee, Jayraan; Cherner, Mariana; Smith, Davey; Maich, Ingrid; Raboni, Sonia Mara; Rotta, Indianara; Barbosa, Francisco Jaime; Heaton, Robert K; Umlauf, Anya; Ellis, Ronald J

    2013-12-01

    HIV-1 clade C isolates show reduced Tat protein chemoattractant activity compared with clade B. This might influence neuropathogenesis by altering trafficking of monocytes into the CNS. A previous study suggested low rates of HIV-associated dementia in clade C-infected individuals. The present study evaluated neurocognitive impairment rates in clade B- and C-infected individuals from the same local population. HIV+ and HIV- participants were recruited from the same geographic region in Southern Brazil. We evaluated neuropsychological (NP) impairment using a screening instrument (the International HIV Dementia Scale (IHDS)), as well as a Brazilian Portuguese adaptation of a comprehensive battery that has demonstrated sensitivity to HIV-associated neurocognitive disorders (HAND) internationally. NP performance in controls was used to generate T scores and impairment ratings by the global deficit score (GDS) method. Clade assignments were ascertained by sequencing pol and env. Blood and cerebrospinal fluid were collected from all HIV+ participants. HIV+ and HIV- participants were comparable on demographic characteristics. HIV+ participants overall were more likely to be impaired than HIV- by the IHDS and the GDS. Clade B- and C-infected individuals were demographically similar and did not differ significantly in rates of impairment. The prevalence of pleocytosis, a marker of intrathecal cellular chemotaxis, also did not differ between clade B and C infections. Clade B and C HIV-infected individuals from the same geographic region, when ascertained using comparable methods, did not differ in their rates of neurocognitive impairment, and there was no evidence of differences in CNS chemotaxis.

  2. Assessing Disease and Mortality among Small Cetaceans Stranded at a World Heritage Site in Southern Brazil

    PubMed Central

    Domiciano, Isabela G.; Domit, Camila; Broadhurst, Matt K.; Koch, Mariana S.; Bracarense, Ana Paula F. R. L.

    2016-01-01

    Cetaceans are considered environmental sentinels and their health often reflects either anthropogenic or natural spatio-temporal disturbances. This study investigated the pathological findings and mortality of small cetaceans with the aim of detecting hazards and monitoring health trends in a high-biodiversity area. Between 2007 and 2012, 218 stranded cetaceans were recorded on the Paraná coast, southern Brazil. Fifty-seven (26.1%) of these animals, including 50 Sotalia guianensis, 2 Pontoporia blainvillei, 2 Stenella frontalis, 1 Stenella longirostris, 1 Tursiops truncatus and 1 Globicephala melas were necropsied and samples were collected for histopathology. Causes of death were determined in 46 of the 57 (80.7%) animals and most (30 or 65.2%) were ascribed to anthropogenic activities, including fisheries bycatch (28/30) and trauma (2/30). The remaining 16 fatalities were considered natural, and attributed to pneumonia (10/16), emaciation (3/16), septicemia (1/16), neonatal pathology (1/16) and choking via food obstruction (1/16). Irrespective of the cause, bronchointerstitial pneumonia, associated with parasitism, lymphadenitis and membranous glomerulonephritis were common findings among all fatalities. These results suggest, that while anthropogenic activities are a leading cause of cetacean strandings in Paraná, underlying pre-existing diseases may contribute towards deaths. Although the studied area is considered a biosphere reserve by UNESCO, complex anthropogenic and natural interactions might be occurring, increasing cetacean susceptibility to hazards. This study may help facilitate developing an effective conservation plan for coastal cetaceans focusing on reducing fisheries interactions, habitat degradation and pollution as mechanisms for ultimately increasing species resilience. PMID:26871703

  3. Seasonal rhythms of seed rain and seedling emergence in two tropical rain forests in southern Brazil.

    PubMed

    Marques, M C M; Oliveira, P E A M

    2008-09-01

    Seasonal tropical forests show rhythms in reproductive activities due to water stress during dry seasons. If both seed dispersal and seed germination occur in the best environmental conditions, mortality will be minimised and forest regeneration will occur. To evaluate whether non-seasonal forests also show rhythms, for 2 years we studied the seed rain and seedling emergence in two sandy coastal forests (flooded and unflooded) in southern Brazil. In each forest, one 100 x 30-m grid was marked and inside it 30 stations comprising two seed traps (0.5 x 0.5 m each) and one plot (2 x 2 m) were established for monthly monitoring of seed rain and a seedling emergence study, respectively. Despite differences in soil moisture and incident light on the understorey, flooded and unflooded forests had similar dispersal and germination patterns. Seed rain was seasonal and bimodal (peaks at the end of the wetter season and in the less wet season) and seedling emergence was seasonal and unimodal (peaking in the wetter season). Approximately 57% of the total species number had seedling emergence 4 or more months after dispersal. Therefore, both seed dormancy and the timing of seed dispersal drive the rhythm of seedling emergence in these forests. The peak in germination occurs in the wetter season, when soil fertility is higher and other phenological events also occur. The strong seasonality in these plant communities, even in this weakly seasonal climate, suggests that factors such as daylength, plant sensitivity to small changes in the environment (e.g. water and nutrient availability) or phylogenetic constraints cause seasonal rhythms in the plants.

  4. Cognition and Indicators of Dietary Habits in Older Adults from Southern Brazil

    PubMed Central

    França, Vivian Francielle; Barbosa, Aline Rodrigues; D’Orsi, Eleonora

    2016-01-01

    Objective To assess the association between unhealthy dietary habits and cognition in older adults from Southern Brazil. Methods This cross-sectional study analyzed data from the second wave of a population- and household-based epidemiological survey (2013–2014) conducted in the city of Florianópolis. A total of 1,197 older adults (778 women) over 60 years old participated in the study. Cognition, the dependent variable, was measured by the Mini-Mental State Examination (MMSE). The independent variables were the following indicators of unhealthy dietary habits: low intake of fruits and vegetables (≤ 4 servings/day); fish (< 1 serving/week); and habitual fatty meat intake (yes/no). Adjustments were made for age, education level, income, smoking status, alcohol intake, leisure-time physical activity, depression symptoms, chronic diseases, and body mass index. Simple and multiple linear regression analyses were performed, considering sampling weights and stratification by gender. Results The mean MMSE scores for men and women were 25.15 ± 5.56 and 24.26 ± 5.68, respectively (p = 0.009). After adjustments, in women low fruit and vegetable intake (≤ 4 servings/day) was independently associated with the lowest MMSE scores. No associations were found in men. Additionally, women’s mean MMSE scores increased as their daily frequency of fruit and vegetable intake increased (p = 0.001). Conclusion Women with low fruit and vegetable intake according to the World Health Organization (WHO) have lower cognition scores. Regular intake of fruits, vegetables, and fish in exchange of fatty meats may be a viable public policy strategy to preserve cognition in aging. PMID:26894259

  5. Nutritional status, physical performance and functional capacity in an elderly population in southern Brazil.

    PubMed

    Danielewicz, Ana Lúcia; Barbosa, Aline Rodrigues; Del Duca, Giovâni Firpo

    2014-01-01

    To investigate the association between nutritional status and functional limitation and disability in an elderly population in southern Brazil. Epidemiological, cross-sectional household-based study carried out with 477 elderly of both sexes (60 to 100 years). Body mass index (BMI) served to assess the nutritional status: underweight (BMI < 22 kg/m2) and overweight (BMI > 27 kg/m2). The sum score (0-5) obtained in three tests: "chair stand" and "pick up a pen" (measured by time) and standing balance (four static measurements) assessed the functional limitation. The disability was evaluated by the difficulty in performing one or more self-reported tasks related to basic activities of daily living (ADLs) and instrumental activities of daily living (IADLs). Crude and adjusted analyzes (3 models) were carried out using Poisson regression; prevalence ratios (PR) and 95% confidence intervals (CI) were calculated. Crude analyzes showed a positive association between underweight and functional limitation (PR = 2.71, 95% CI = 1.63 to 4.51); overweight and disability in ADLs (PR = 2.20, CI 95% = 1.44 to 3.35); overweight and disability in IADLs (PR = 1.56, CI 95% = 1.20 to 2.03). The additional adjustments for gender, age, level of education, living arrangements, current work, cognitive function and number of morbidities reduced the strength of the associations, without changing the statistical strength. Nutritional status is a factor that is independently and positively associated with functional limitation and disability. We recommend the use of this indicator to monitor the health of the elderly.

  6. An agro-climatic approach to determine citrus postbloom fruit drop risk in Southern Brazil

    NASA Astrophysics Data System (ADS)

    Soares-Colletti, Ana R.; Alvares, Clayton A.; Sentelhas, Paulo C.

    2016-06-01

    Postbloom fruit drop (PFD) causes lesions on the petals of citrus flowers and induces fruit abscission causing severe damage to production when the flowering period coincides with intense rainfall. The aims of this study were to develop a phenological-climatological model for citrus PFD occurrence and, together with weather data series from several locations, to determine and map the agro-climatic favorability of PFD occurrence in the state of São Paulo, Southern Brazil. A phenological flowering model was developed to identify when citrus flowering occurs. The flowering starts after when a temperature below 10 °C in the months of June or July is reached followed by cumulative rainfall within 5 days of at least 20 mm, and then 96 °C days. Between the beginning of flowering and its peak, 147 °C days are required, and between the peak and its end, approximately 229 °C days, being 206 °C days from the peak to the moment when flowers remaining are about 50 % of total. The relationship between PFD incidence and accumulated rainfall during the critical period (between flowering peak and 50 % of flowers remaining) was adjusted by the Gompertz model ( R 2 = 0.99, p < 0.05). After its validation, this model was used to estimate PFD incidence for 29 locations in the state, from 1993 to 2013, which allowed to map the PFD climatic favorability for the state through a Geographical Information System using linear models based on latitude, longitude, and altitude. The obtained map showed a trend of PFD incidence increasing from the northwest of the state of São Paulo towards the south and the coastal region, with medium to very high favorability in the center of the state. The results of this study can be used by growers as a guide for disease control planning as well as for defining the regions where the climatic conditions are likely to escape this disease.

  7. Assessing Disease and Mortality among Small Cetaceans Stranded at a World Heritage Site in Southern Brazil.

    PubMed

    Domiciano, Isabela G; Domit, Camila; Broadhurst, Matt K; Koch, Mariana S; Bracarense, Ana Paula F R L

    2016-01-01

    Cetaceans are considered environmental sentinels and their health often reflects either anthropogenic or natural spatio-temporal disturbances. This study investigated the pathological findings and mortality of small cetaceans with the aim of detecting hazards and monitoring health trends in a high-biodiversity area. Between 2007 and 2012, 218 stranded cetaceans were recorded on the Paraná coast, southern Brazil. Fifty-seven (26.1%) of these animals, including 50 Sotalia guianensis, 2 Pontoporia blainvillei, 2 Stenella frontalis, 1 Stenella longirostris, 1 Tursiops truncatus and 1 Globicephala melas were necropsied and samples were collected for histopathology. Causes of death were determined in 46 of the 57 (80.7%) animals and most (30 or 65.2%) were ascribed to anthropogenic activities, including fisheries bycatch (28/30) and trauma (2/30). The remaining 16 fatalities were considered natural, and attributed to pneumonia (10/16), emaciation (3/16), septicemia (1/16), neonatal pathology (1/16) and choking via food obstruction (1/16). Irrespective of the cause, bronchointerstitial pneumonia, associated with parasitism, lymphadenitis and membranous glomerulonephritis were common findings among all fatalities. These results suggest, that while anthropogenic activities are a leading cause of cetacean strandings in Paraná, underlying pre-existing diseases may contribute towards deaths. Although the studied area is considered a biosphere reserve by UNESCO, complex anthropogenic and natural interactions might be occurring, increasing cetacean susceptibility to hazards. This study may help facilitate developing an effective conservation plan for coastal cetaceans focusing on reducing fisheries interactions, habitat degradation and pollution as mechanisms for ultimately increasing species resilience.

  8. Vital Pulp Therapies in Clinical Practice: Findings from a Survey with Dentist in Southern Brazil.

    PubMed

    Chisini, Luiz Alexandre; Conde, Marcus Cristian Muniz; Correa, Marcos Britto; Dantas, Raquel Venâncio Fernandes; Silva, Adriana Fernandes; Pappen, Fernanda Geraldes; Demarco, Flávio Fernando

    2015-01-01

    Studies based on dentists' clinical practice possess vital relevance to understand factors leading the clinicians to choose by a specific technique over another. This study investigated which clinical conduct therapies are adopted by dentists in front of deep caries. Was evaluated how the place of work, post-graduate training and years since complete graduation influenced their decisions. A cross-sectional study was performed using a self-applied questionnaire with dentists (n=276) in Southern Brazil. Information regarding post-graduation training (specialization, master's or PhD), clinical experience (years since completing graduation) and place of work were investigated. The information regarding pulp vital therapies (materials for direct pulp capping; techniques for caries removal in deep cavities and strategies for indirect pulp capping) were collected by specific questions. Data were submitted to descriptive analysis and Exact Fischer Test. Response rate was 68% (187). The majority of dentists selected the calcium hydroxide (CH) as first material for direct (86.3%) and indirect (80.3%) pulp protection. Partial caries removal was reported by 61.9% of dentists. Less experienced clinical dentists choose partial caries removal more frequently (p=0.009), if compared with dentists graduated 10 years and up ago. The use of MTA was more common among professionals working at academic environment. Besides, MTA was not mentioned by professionals working exclusively in the public health service (p=0.003). In conclusion, the time since graduation influenced the clinical conduct related to caries removal. The choice of liner materials was influenced by dentists' workplace.

  9. Recent transmission of drug-resistant Mycobacterium tuberculosis in a prison population in southern Brazil.

    PubMed

    Reis, Ana Julia; David, Simone Maria Martini de; Nunes, Luciana de Souza; Valim, Andreia Rosane de Moura; Possuelo, Lia Gonçalves

    2016-01-01

    We conducted a cross-sectional, retrospective study, characterized by classical and molecular epidemiology, involving M. tuberculosis isolates from a regional prison in southern Brazil. Between January of 2011 and August of 2014, 379 prisoners underwent sputum smear microscopy and culture; 53 (13.9%) were diagnosed with active tuberculosis. Of those, 8 (22.9%) presented with isoniazid-resistant tuberculosis. Strain genotyping was carried out by 15-locus mycobacterial interspersed repetitive unit-variable-number tandem-repeat analysis; 68.6% of the patients were distributed into five clusters, and 87.5% of the resistant cases were in the same cluster. The frequency of drug-resistant tuberculosis cases and the rate of recent transmission were high. Our data suggest the need to implement an effective tuberculosis control program within the prison system. RESUMO Estudo transversal, retrospectivo, com isolados de M. tuberculosis de pacientes de um presídio regional no sul do Brasil, caracterizado através de epidemiologia clássica e molecular. Entre janeiro de 2011 e agosto de 2014, 379 detentos foram submetidos a baciloscopia e cultura, sendo 53 (13,9%) diagnosticados com tuberculose ativa. Desses, 8 (22,9%) apresentavam tuberculose resistente a isoniazida. A genotipagem das cepas foi realizada por 15-locus mycobacterial interspersed repetitive units-variable number of tandem repeat analysis; 68,6% dos pacientes estavam distribuídos em cinco clusters, e 87,5% dos casos resistentes estavam em um mesmo cluster. Verificou-se uma frequência elevada de casos de resistência e alta taxa de transmissão recente. Estes dados sugerem a necessidade da implantação de um programa efetivo de controle da tuberculose no sistema prisional.

  10. Socio-economic life course and obesity among adults in Florianopolis, southern Brazil.

    PubMed

    Wagner, Katia Jakovljevic Pudla; Bastos, João Luiz; Navarro, Albert; Boing, Antonio Fernando

    2017-03-29

    To estimate the association between socio-economic life course and body mass index (BMI), waist circumference (WC) and general and abdominal obesity in adults. A cross-sectional analysis of a population-based cohort study of 1,222 adults (aged 22-63) from Florianopolis, southern Brazil. The socio-economic life course was analysed using the educational level of participants and their parents. Height, weight and WC were measured by specially trained staff. Linear and logistic regressions were used with adjustment for confounding factors, and data were stratified according to sex. Mean BMI and WC were about 2kg/m(2) (95% CI: -3.3 to -0.7) and 6cm (95% CI: -9.7 to -2.9) lower in women with a high socio-economic position, while the association was reversed in men with a high socio-economic position, with WC being about 4cm higher (95% CI: 0.1 to 7.5). In addition, women who had always been in a high socio-economic position were less likely to have abdominal obesity (OR: 0.38; 95% CI: 0.20 to 0.76) while no such association was found in men. Socio-economic life course influences BMI, WC and obesity, with differences between males and females, thereby indicating that public policies that contemplate a socio-economic life course approach can be effective for controlling obesity. Copyright © 2017 SESPAS. Publicado por Elsevier España, S.L.U. All rights reserved.

  11. Plants used as antidiabetics in popular medicine in Rio Grande do Sul, southern Brazil.

    PubMed

    Trojan-Rodrigues, M; Alves, T L S; Soares, G L G; Ritter, M R

    2012-01-06

    Plants are widely as antidiabetics. The study of these plants is essential because many of them may have undesirable effects, such as acute or chronic toxicity; or their use may even delay or discourage the adoption of the proper and effective treatment. The present study surveyed the plant species that are popularly used to treat diabetes mellitus in the state of Rio Grande do Sul in southern Brazil. Sixteen ethnobotanical surveys performed in the state were consulted, and the species used to treat diabetes were listed. For species cited in at least two of the studies, scientific data related to antidiabetic activity were searched in the ISI Knowledge database. The scientific binomial of each species was used as keywords, and data found in review papers were also included. A total of 81 species in 42 families were mentioned; the most important families were Asteraceae and Myrtaceae. Twenty eight species were cited at least twice as being used to treat diabetes in the state. For 11 of these, no scientific data regarding antidiabetic activity could be located. The species most frequently mentioned for use with diabetes were Syzygium cumini (Myrtaceae) and Bauhinia forficata (Fabaceae), in 12 studies each, followed by Sphagneticola trilobata (Asteraceae), in six studies; and Baccharis trimera (Asteraceae), Bidens pilosa (Asteraceae), Cynara scolymus (Asteraceae), and Leandra australis (Melastomataceae) in four studies each. Bauhinia forficata and Syzygium cumini have been studied in more detail for antidiabetic activity. A considerable number of plant species are traditionally used for the treatment of diabetes melitus in the Rio Grande do Sul State. The majority of those plants that have been studied for antidiabetic activity showed promising results, mainly for Bauhinia forficata and Syzygium cumini. However, for most of the plants mentioned, the studies are not sufficient to guarantee the efficacy and safety in the use of these plants in the treatment against

  12. Serological survey of Toxoplasma gondii in captive Neotropical felids from Southern Brazil.

    PubMed

    Ullmann, Leila Sabrina; da Silva, Rodrigo Costa; de Moraes, Wanderlei; Cubas, Zalmir Silvino; dos Santos, Leonilda Correia; Hoffmann, Juliano Leônidas; Moreira, Nei; Guimaraes, Ana Marcia Sa; Montaño, Patrícia; Langoni, Helio; Biondo, Alexander Welker

    2010-08-27

    Toxoplasma gondii is the causative intracellular protozoan of toxoplasmosis in human being and animals. Members of the Felidae family are considered the single definitive host for the infection; both wild and domestic cats are able to excrete oocysts in the environment. Wild cats maintained in captivity may serve as source of infection for other clinically susceptible animals in the same environment. The aim of this study was to determine the frequency of T. gondii IgG antibodies in 57 neotropical felids (1 Leopardus geoffroyi; 3 Puma yagouaroundi; 17 Leopardus wiedii; 22 Leopardus tigrinus; and 14 Leopardus pardalis) kept at the Bela Vista Biological Sanctuary, Itaipu Binacional, Southern Brazil, by the modified agglutination test (MAT) using titer 16 as cut-off point. Seropositivity was observed in 38/57 (66.67%; 95% CI 53.66-77.51%) samples, with higher frequency in ocelots (71.43%). Wild-caught felids were three times more likely to be infected when compared to zoo-born animals (P

  13. Socioeconomic inequalities in expenditures and income committed to the purchase of medicines in Southern Brazil.

    PubMed

    Boing, Alexandra Crispim; Bertoldi, Andréa Dâmaso; Peres, Karen Glazer

    2011-10-01

    To describe socioeconomic inequalities regarding the use, expenditures and the income committed to the purchase of medicines. A cross-sectional population-based study was carried out with 1,720 adults living in the urban area of Florianópolis, Southern Brazil, in 2009. Cluster sampling was adopted and census tracts were the primary sampling units. Use of medicines and the expenditures incurred in their purchase in the past 30 days were investigated through interviews. Use, expenditures and the income committed concerning medicines were analyzed according to per capita family income, self-reported skin color, age and sex, adjusting for the complex sample. The prevalence of medicine use was 76.5% (95%CI: 73.8; 79.3), higher among women and in older individuals. The mean expenditure on medicine was R$ 46.70, with higher values among women, whites, older individuals and among richer people. While 3.1% of the richest committed more than 15% of their income to purchasing medicine, that figure reached 9.6% in the poorest group. The proportion of people that had to buy medicines after an unsuccessful attempt to obtain them in the public health system was higher among the poor (11.0%), women (10.2%) and the elderly (11.1%). A large part of the adults bought medicines contained in the National List of Essential Medicines (19.9%) or in the Municipal List of Essential Medicines (28.6%), with significant differences according to gender, age and income. There is socioeconomic, age and gender inequality in the income committed to the purchase of medicines, with worse conditions for the poor, older individuals and women.

  14. [Rapid evaluation of ant diversity in land use systems in southern Bahia, Brazil].

    PubMed

    Braga, Danielle L; Louzada, Júlio N C; Zanetti, Ronald; Delabie, Jacques

    2010-01-01

    We aimed to compare the soil ant diversity in different land use systems from Atlantic Forest area, in Southern Bahia state, Brazil. The ants were sampled in 16 sites: two primary forest sites (un-logged forest); three young secondary forests (<8 years old); three intermediate secondary forests (8-20 years old); three old secondary forests (>20 years old); three Eucalyptus grandis plantations (3-7 years old), and two introduced pastures. Each site was sampled in three sampling points 15 m apart, and distant over 50 m from the site edge. In each sampling point we gathered the litter from a 1 m² and extracted the ants with Winkler extractors during 48h. We found 103 ant species from 29 genera and eight subfamilies. The five richest genera were Pheidole (19 species), Solenopsis (8), Apterostigma (10), Hypoponera (7) e Paratrechina (5). The highest ant richness density was found in the primary forest (7.4 species/sample; S = 37; n = 5); followed by the old secondary forest (5.33 species/sample; S = 48; n = 9); young secondary forest (5.25 species/sample; S = 42, n = 8); eucalyptus plantation (4.22 species/sample; S = 38, n = 9), intermediate secondary forest (3.5 species/sample; S = 35, n = 10, and introduced pasture (2.67 species/sample; S = 16, n = 6). The ecosystems with higher structural complexity showed the highest ant richness density by sample. Therefore, in the Atlantic Forest region, the eucalyptus plantation is a better alternative of land use to conserve the ant biodiversity than pastures, and quite similar to native secondary forests in ant community characteristics.

  15. Risk and other factors associated with toxoplasmosis and toxocariasis in pregnant women from southern Brazil.

    PubMed

    Santos, P C; Telmo, P L; Lehmann, L M; Mattos, G T; Klafke, G B; Lorenzi, C; Hirsch, C; Lemos, L; Berne, M E A; Gonçalves, C V; Scaini, C J

    2017-09-01

    Toxoplasmosis causes complications during pregnancy that have serious effects on fetal development. Thus far, toxocariasis has been reported to spread only via vertical transmission. Nonetheless, the population of pregnant women is also exposed to this infection. Co-infection with both Toxoplasma gondii and Toxocara spp. has been reported in children, but there are no reports of co-infection in the population of pregnant women. The aim of this study was to determine the prevalence of co-infection with T. gondii and Toxocara spp. in pregnant women at a university hospital in southern Brazil, and to identify the risk factors associated with infection by both parasites. Two hundred pregnant women were tested for the presence of anti-T. gondii and anti-Toxocara spp. antibodies and were asked to complete an epidemiological questionnaire. In this study, the co-infection rate observed in the total population of pregnant women was 8%. In addition, women with a positive result for a serology test for Toxocara spp. were at increased risk of infection by T. gondii (P = 0.019). Co-infection with both parasites in pregnant women was associated with low birth weights in neonates. The similar modes of transmission of both parasites could explain the co-infection. Only a few previous studies have investigated this phenomenon. The findings of the present study emphasize the importance of serological diagnosis during prenatal care and further research in this area to identify risk factors associated with this co-infection, and the possible implications of this co-infection during pregnancy and on the health of newborns.

  16. HIV/AIDS risk among female sex workers who use crack in Southern Brazil.

    PubMed

    Malta, Monica; Monteiro, Simone; Lima, Rosa Maria Jeronymo; Bauken, Suzana; Marco, Aliamar de; Zuim, Gleisse Cristine; Bastos, Francisco Inacio; Singer, Merrill; Strathdee, Steffanie Anne

    2008-10-01

    To understand the social context of female sex workers who use crack and its impact on HIV/AIDS risk behaviors. METHODODOLOGICAL PROCEDURES: Qualitative study carried out in Foz do Iguaçu, Southern Brazil, in 2003. Twenty-six in-depth interviews and two focus groups were carried out with female commercial sex workers who frequently use crack. In-depth interviews with health providers, community leaders and public policy managers, as well as field observations were also conducted. Transcript data was entered into Atlas.ti software and grounded theory methodology was used to analyze the data and develop a conceptual model as a result of this study. Female sex workers who use crack had low self-perceived HIV risk in spite of being engaged in risky behaviors (e.g. unprotected sex with multiple partners). Physical and sexual violence among clients, occasional and stable partners was widespread jeopardizing negotiation and consistent condom use. According to health providers, community leaders and public policy managers, several female sex workers who use crack are homeless or live in slums, and rarely have access to health services, voluntary counseling and testing, social support, pre-natal and reproductive care. Female sex workers who use crack experience a plethora of health and social problems, which apparently affect their risks for HIV infection. Low-threshold, user-friendly and gender-tailored interventions should be implemented, in order to increase the access to health and social-support services among this population. Those initiatives might also increase their access to reproductive health in general, and to preventive strategies focusing on HIV/AIDS and other sexually transmitted infections.

  17. Food expenditures, cariogenic dietary practices and childhood dental caries in southern Brazil.

    PubMed

    Feldens, C A; Rodrigues, P H; Rauber, F; Chaffee, B W; Vitolo, M R

    2013-01-01

    Family expenditures on food for children may represent an important barrier to the adoption of healthy feeding practices in populations of low socioeconomic status. The aim of this study was to explore the relationship between cariogenic feeding practices, expenditures on food for children and dental caries. This cross-sectional study included 329 four-year-old children from São Leopoldo in southern Brazil. Cariogenic dietary practices were assessed at 4 years of age using two 24-hour recalls conducted with the children's mothers. Expenditures on food for children were estimated based on all reported food items and the respective amounts ingested. Early childhood caries and severe early childhood caries were assessed by clinical examination at 4 years of age. Cariogenic dietary habits were not associated with lower food expenditures. On the contrary, in multivariable regression analysis, the intake of chocolate (p = 0.007), soft drinks (p = 0.027) and a higher number of meals and snacks per day (p < 0.001) was associated with greater expenditures on food for children. No statistically significant differences were observed in food expenditures or in the proportion of household income spent on feeding children between caries-free children, those with early childhood caries and those with severe early childhood caries. In conclusion, keeping children free of dental caries does not necessarily increase food expenditures or the proportion of household income spent on feeding children in low-socioeconomic status populations. Some cariogenic dietary practices were associated with greater expenditures on child feeding. Copyright © 2013 S. Karger AG, Basel.

  18. Health implications of atmospheric aerosols from asbestos-bearing road pavements traditionally used in Southern Brazil.

    PubMed

    Godoi, Ricardo H M; Gonçalves, Sérgio J; Sayama, Célia; Polezer, Gabriela; Reis Neto, José M; Alföldy, Bálint; Van Grieken, René; Riedi, Carlos A; Yamamoto, Carlos I; Godoi, Ana F L; Bencs, László

    2016-12-01

    Serpentine and amphibole asbestos occur naturally in certain geologic settings worldwide, most commonly in association with ultramafic rocks, along associated faults. Ultramafic rocks have been used in Piên County, Southern Brazil for decades for the purpose of road paving in rural and urban areas, but without the awareness of their adverse environmental and health impact. The aim of this study was the chemical characterization of aerosols re-suspended in two rural roads of Piên, paved with ultramafic rocks and to estimate the pulmonary deposition of asbestos aerosols. Bulk aerosol samples were analyzed by means of X-ray fluorescence spectrometry and X-ray diffraction analysis, in order to characterize elemental composition and crystallinity. Single-particle compositions of aerosols were analyzed by computer-controlled electron-probe microanalysis, indicating the presence of a few percentages of serpentine and amphibole. Given the chemical composition and size distribution of aerosol particles, the deposition efficiency of chrysotile, a sub-group of serpentine, in two principal segments of the human respiratory system was estimated using a lung deposition model. As an important finding, almost half of the inhaled particles were calculated to be deposited in the respiratory system. Asbestos depositions were significant (∼25 %) in the lower airways, even though the selected breathing conditions (rest situation, nose breathing) implied the lowest rate of respiratory deposition. Considering the fraction of inhalable suspended chrysotile near local roads, and the long-term exposure of humans to these aerosols, chrysotile may represent a hazard, regarding more frequent development of lung cancer in the population of the exposed region.

  19. Ecological traits of two sympatric species of Hyalella Smith, 1874 (Crustacea, Amphipoda, Dogielinotidae) from southern Brazil

    NASA Astrophysics Data System (ADS)

    da Silva Castiglioni, Daniela; Buckup, Georgina Bond

    2008-01-01

    This work was performed with the aim to test theoretical predictions regarding that the sympatric species Hyalella pleoacuta and H. castroi might show distinct population biology to facilitate its coexistence. The specimens were collected monthly with nets from August 2003 through July 2004 in two trout ponds at Sítio Vale das Trutas locality, São José dos Ausentes County, southern Brazil. In the laboratory, the specimens were measured as cephalothorax length (mm), being the sex and ovigerous conditions checked. The species H. pleoacuta was 2.94 times more frequent than H. castroi. Males were significantly greater in size than females ( H. pleoacuta—males: 0.74 ± 0.002 mm and females: 0.66 ± 0.001 mm; H. castroi—males: 0.84 ± 0.00 mm and females: 0.72 ± 0.003 mm). Males and females of H. castroi showed a greater mean body size than H. pleoacuta. Sexual maturity was attained at 0.53 mm in males and 0.48 mm in females of H. pleoacuta, and 0.72 mm in males and 0.67 mm in females of H. castroi. The frequency distribution in size classes was usually bimodal in H. pleoacuta and polymodal in H. castroi throughout the year. Sex ratio was female-biased in either species of Hyalella. Ovigerous females (carrying eggs or juveniles in the marsupium) were collected throughout the year in both Hyalella species, but H. pleoacuta and H. castroi were found with more frequency during the winter and fall, respectively. Recruitment occurred in all months of sampling, the juvenile frequency being more than 50% of the amphipods collected in almost all months in both species. The biological differences (especially body size, size at sexual maturity, number of specimens collected and reproductive peak) and microhabitat specialization can be facilitating factors in the coexistence of H. pleoacuta and H. castroi in artificial ponds raising trout.

  20. Prevalence and predictors associated with intestinal infections by protozoa and helminths in southern Brazil.

    PubMed

    Casavechia, Maria Teresinha Gomes; Lonardoni, Maria Valdrinez Campana; Venazzi, Eneide Aparecida Sabaini; Campanerut-Sá, Paula Aline Zanetti; da Costa Benalia, Hugo Rafael; Mattiello, Matheus Felipe; Menechini, Pedro Victor Lazaretti; Dos Santos, Carlos Aparecido; Teixeira, Jorge Juarez Vieira

    2016-06-01

    Approximately 2 billion people are infected with soil-transmitted helminths worldwide, mainly in tropical and subtropical areas. This research aimed to investigate the prevalence and predictors associated with parasitic infections in primary health care. A cross-sectional study was performed with a large random sample to identify the prevalence and predictors associated with parasitic infections in primary health care in Marialva, southern Brazil, from April 2011 to September 2013. Stool samples from 775 individuals were analyzed for the presence of protozoan cysts, helminth eggs, and larvae. The overall prevalence of intestinal parasites was 13.94 %, and the prevalence of protozoa and helminths was 15.1 and 2.9 %, respectively. The predictor variables that were associated with intestinal parasites were male gender odds ratio (OR) 1.60, 95 % confidence interval (CI 1.10-2.40) and the absence of a kitchen garden (OR 2.28, 95 % CI, 1.08-4.85). Positive associations were found between Giardia duodenalis and individuals aged ≤18 with high risk (OR 19.0, 95 % CI 2.16-167.52), between Endolimax nana and the absence of a kitchen garden (p < 0.01), and between Trichuris trichiura and the presence of a kitchen garden (p = 0.014). Polyparasitism was present in 27.27 % of infected individuals. Our findings confirmed a relatively low prevalence in primary care, compared to international standards, despite the rare publications in the area. As variables, male gender and the absence of a kitchen garden stood out as important predictors. It is highly relevant that the health conditions of the population comply with consistent standards.

  1. Anthropometric indices among schoolchildren from a municipality in Southern Brazil: a descriptive analysis using the LMS method☆

    PubMed Central

    Barbosa, Valter Cordeiro; Lopes, Adair da Silva; Fagundes, Ricardo Rosa; de Campos, Wagner

    2014-01-01

    OBJECTIVE: To describe the percentile values for body mass index (BMI), waist circumference (WC) and waist-to-height (WHtR) of children from Colombo, Brazil, and compare them with data of children from other countries. METHODS: This was a cross-sectional study with a random sample of 2,035 children aged 6-11 years. Age- and sex-specific smoothed percentiles curves for BMI, WC and WHtR were created using the LMS method. Values of 10th, 50th and 90th percentiles from Brazilian children were compared with data from other countries. RESULTS: There was a trend of increasing BMI and WC with age in both sexes. WHtR remained constant with advancing age in boys and girls. Comparison of the growth pattern among countries showed clear differences. Southern Brazil boys and girls had elevated 90th percentile values for BMI, which was similar to German children and higher than the North American and World Health Organization percentile values. However, children from this study had intermediate values for WC and WHtR in comparison to children from other countries. CONCLUSIONS: Elevated BMI values were observed among southern Brazilian children, but WC and WHtR percentile values were lower in southern Brazilian children than in children from other countries. Interventions at different levels should be made to avoid a probable increase of nutritional disorders (especially general obesity) in the next years. PMID:25510997

  2. Phylogenetic characterization of hantaviruses from wild rodents and hantavirus pulmonary syndrome cases in the state of Parana (southern Brazil).

    PubMed

    Raboni, Sonia Mara; Hoffmann, Federico G; Oliveira, Renata C; Teixeira, Bernardo R; Bonvicino, Cibele R; Stella, Vanessa; Carstensen, Suzana; Bordignon, Juliano; D'Andrea, Paulo S; Lemos, Elba R S; Duarte Dos Santos, Claudia Nunes

    2009-09-01

    Over 1,100 cases of hantavirus pulmonary syndrome (HPS) have occurred in Brazil since 1993, but little is known about Brazilian hantaviruses, and many of their rodent hosts remain unknown. The Araucaria hantavirus (ARAUV) was described recently from HPS patients from Paraná, in southern Brazil, but its host could not be identified. In this study, rodents were captured from regions with high HPS prevalence to address this issue. ARAUV RNA was detected in three distantly related rodent species: Oligoryzomys nigripes, Oxymycterus judex and Akodon montensis. Furthermore, a specimen of A. montensis was infected with a Jaborá-like virus, implying that A. montensis can be infected by at least two different hantaviruses. The presence of the same hantavirus strain in three different rodent species and the co-circulation of two different strains in the same rodent species highlight the potential for genomic reassortment, which could have an impact on hantavirus transmission dynamics in nature and on human epidemiology.

  3. Incidence of canine leptospirosis in the metropolitan area of Curitiba, State of Paraná, Southern Brazil.

    PubMed

    Martins, Camila Marinelli; Barros, Cristiane da Conceição de; Galindo, Claudia Martins; Kikuti, Mariana; Ullmann, Leila Sabrina; Pampuch, Raquel dos Santos; Hoffmann, Juliano Leônidas; Langoni, Helio; Ferreira, Fernando; Molento, Marcelo Beltrão; Biondo, Alexander Welker

    2013-01-01

    The incidence of canine leptospirosis in Brazil needs to be assessed. The same dogs in southern Brazil were sampled over two years to determine the prevalence, incidence and association of canine leptospirosis with various risk factors. In 2009, the prevalence was 33 (14.4%) of 228 dogs, with a predominance of serovar Canicola (33.4%). In 2010, 90 dogs were re-evaluated (the remaining dogs were lost to deaths, address changes and donations), and the prevalence was found to be 35 (38.9%) of 90, with the predominant serovar being Icterohaemorrhagiae (51.4%). Moreover, the incidence was 26 of 90 (28.9%), and the disease was statistically associated with age (2009) and street access (2010). Our findings revealed instability in the dog population and age to be relevant risk factors for canine leptospirosis.

  4. Population-based HIV prevalence and associated factors in male-to-female transsexuals from Southern Brazil.

    PubMed

    Costa, Angelo Brandelli; Fontanari, Anna Martha Vaitses; Jacinto, Michelle Moraes; da Silva, Dhiordan Cardoso; Lorencetti, Emilaine Karine; da Rosa Filho, Heitor Tomé; Mueller, Andressa; de Garcia, Claudia Garcia; Nardi, Henrique Caetano; Koller, Silvia Helena; Lobato, Maria Inês Rodrigues

    2015-02-01

    This study assessed HIV prevalence and associated factors in 284 male-to-female transsexuals from southern Brazil. Seroprevalence was 25 %. Seroprevalence was higher and associated with older age, residence in the metropolitan area, history of diagnosis of other STDs, and reported history of sex work. The year of diagnosis showed no significant relationship with the prevalence of HIV nor the fact of being in a stable relationship, a history of drug use, years of education, and race/ethnicity. The odds of HIV infection compared with the general Brazilian population was 55.55 (95 % CI 38.39-80.39). Changes in the views of the vulnerable groups to HIV/AIDS in Brazil and efforts in the construction of strategies of prevention and in the guarantee of human rights are required.

  5. Modeling monthly meteorological and agronomic frost days, based on minimum air temperature, in Center-Southern Brazil

    NASA Astrophysics Data System (ADS)

    Alvares, Clayton Alcarde; Sentelhas, Paulo César; Stape, José Luiz

    2017-09-01

    Although Brazil is predominantly a tropical country, frosts are observed with relative high frequency in the Center-Southern states of the country, affecting mainly agriculture, forestry, and human activities. Therefore, information about the frost climatology is of high importance for planning of these activities. Based on that, the aims of the present study were to develop monthly meteorological (F MET) and agronomic (F AGR) frost day models, based on minimum shelter air temperature (T MN), in order to characterize the temporal and spatial frost days variability in Center-Southern Brazil. Daily minimum air temperature data from 244 weather stations distributed across the study area were used, being 195 for developing the models and 49 for validating them. Multivariate regression models were obtained to estimate the monthly T MN, once the frost day models were based on this variable. All T MN regression models were statistically significant (p < 0.001), presenting adjusted R 2 between 0.69 and 0.90. Center-Southern Brazil is mainly hit by frosts from mid-fall (April) to mid-spring (October). The period from November to March is considered as frost-free, being very rare a frost day within that period. Monthly F MET and F AGR presented significant sigmoidal relationships with T MN (p < 0.0001), with adjusted R 2 above of 0.82. The residuals of the frost day models were random, which means that the sigmoidal models performed quite well for interpreting the frost day variability throughout the study area. The highlands of Santa Catarina, Rio Grande do Sul, São Paulo, and Minas Gerais had in average more than 25 and 13 frosts per year, respectively, for F MET and F AGR. The F MET and F AGR maps developed in this study for Center-Southern Brazil is a useful tool for farmers, foresters, and researchers, since they contribute to reduce frost spatial and temporal uncertainty, helping in planning project for strategic purposes. Furthermore, the monthly F MET and F AGR maps

  6. Molecular characterization and clinical epidemiology of human respiratory syncytial virus (HRSV) A and B in hospitalized children, Southern Brazil.

    PubMed

    Moreira, Francielli B; Rosario, Cristine S; Santos, Jucelia S; Avanzi, Valéria M; Nogueira, Meri Bordignon; Vidal, Luine R; Raboni, Sonia Mara

    2017-02-18

    Human respiratory syncytial virus (HRSV) is a major etiologic agent of pediatric respiratory infections. Genetic variability of its glycoprotein G enables HRSV to evade the immune response and determines its seasonal dissemination. This study reports genetic variability and clinical profiles of HRSV-infected patients from Southern Brazil. Seventy positive samples, 78% type A and 22% type B, were analyzed. Of the patients (median age, 6 months; interquartile range, 2-11 years), 16% had co-morbidities and 17% developed severe disease. The ON1 HRSV genotype first appeared in 2012, and patients infected with this genotype showed an increased tendency to develop severe disease.

  7. A new species of tree crickets Oecanthus (Orthoptera, Gryllidae, Oecanthinae) in tobacco plantation from Southern Brazil, with body color variation.

    PubMed

    Milach, Elisa Machado; Martins, Luciano De P; Da Costa, Maria Kátia Matiotti; Gottschalk, Marco Silva; De Oliveira, Gabriel Lobregat; Redü, Darlan Rutz; Neutzling, Alexandre Schneid; Dornelles, José Eduardo Figueiredo; Vasconcellos, Lucas Azevedo; Zefa, Edison

    2015-09-15

    We provide herein a description of a new species of Oecanthus collected from the tobacco plantation in southern Brazil, municipality of São Lourenço do Sul, State of Rio Grande do Sul. Description focused metanotal gland features, phallic sclerites, and calling song. A large sampling of individuals was distributed into four groups according to body and appendages color and dotted. We also rank all kind of marks present in the scape and pedicel. We compare linear morphometric variables of the metanotal glands and tegmina, as well as calling song parameters between groups in order do define all of them as the same species. Photographs and measurements are provided.

  8. Contributions to the Dart versus Arrow Debate: New Data from Holocene Projectile Points from Southeastern and Southern Brazil.

    PubMed

    Okumura, Mercedes; Araujo, Astolfo G M

    2015-01-01

    Lithic bifacial points are very common in the southern and southeastern regions of the Brazilian territory. Dated from Early to Late Holocene, these artifacts have not been studied in terms of their propulsion system. Given the characteristics of the bow and arrow compared to the atlatl and dart, there are important differences in the size and weight of arrowheads and dart points. Applying the techniques proposed by Shott (1997), Bradbury (1997), Fenenga (1953), Hughes (1998), and Hildebrandt and King (2012) to specimens recovered from eight sites dating from the early to the late Holocene, this work aims to present preliminary results to better understand the potential presence of darts and arrows in southeastern and southern Brazil. There was a variation in the results according to the application of different techniques. At least one set of points, dated from the Early Holocene, presented quite a high proportion of specimens classified as arrows, indicating the presence of points that could be used as arrowheads.

  9. Wind Power Energy in Southern Brazil: evaluation using a mesoscale meteorological model

    NASA Astrophysics Data System (ADS)

    Krusche, Nisia; Stoevesandt, Bernhard; Chang, Chi-Yao; Peralta, Carlos

    2015-04-01

    In recent years, several wind farms were build in the coast of Rio Grande do Sul state. This region of Brazil was identified, in wind energy studies, as most favorable to the development of wind power energy, along with the Northeast part of the country. Site assessments of wind power, over long periods to estimate the power production and forecasts over short periods can be used for planning of power distribution and enhancements on Brazil's present capacity to use this resource. The computational power available today allows the simulation of the atmospheric flow in great detail. For instance, one of the authors participated in a research that demonstrated the interaction between the lake and maritime breeze in this region through the use of a atmospheric model. Therefore, we aim to evaluate simulations of wind conditions and its potential to generate energy in this region. The model applied is the Weather Research and Forecasting , which is the mesoscale weather forecast software. The calculation domain is centered in 32oS and 52oW, in the southern region of Rio Grande do Sul state. The initial conditions of the simulation are taken from the global weather forecast in the time period from October 1st to October 31st, 2006. The wind power potential was calculated for a generic turbine, with a blade length of 52 m, using the expression: P=1/2*d*A*Cp*v^3, where P is the wind power energy (in Watts), d is the density (equal to 1.23 kg/m^3), A is the area section, which is equal to 8500 m2 , and v is the intensity of the velocity. The evaluation was done for a turbine placed at 50 m and 150 m of height. A threshold was chosen for a turbine production of 1.5 MW to estimate the potential of the site. In contrast to northern Brazilian region, which has a rather constant wind condition, this region shows a great variation of power output due to the weather variability. During the period of the study, at least three frontal systems went over the region, and thre was a

  10. Gamma-ray spectrometry of granitic suites of the Paranaguá Terrane, Southern Brazil

    NASA Astrophysics Data System (ADS)

    Weihermann, Jessica Derkacz; Ferreira, Francisco José Fonseca; Cury, Leonardo Fadel; da Silveira, Claudinei Taborda

    2016-09-01

    The Paranaguá Terrane, located in the coastal portion of the states of Santa Catarina, Paraná and São Paulo in Southern Brazil is a crustal segment constituted mainly by an igneous complex, with a variety of granitic rocks inserted into the Serra do Mar ridge. The average altitude is approximately 1200 m above sea level, with peaks of up to 1800 m. Due to the difficulty of accessing the area, a shortage of outcrops and the thick weathering mantle, this terrane is understudied. This research aims to evaluate the gamma-ray spectrometry data of the granitic suites of the Paranaguá Terrane, in correspondence with the geological, petrographical, lithogeochemical, relief and mass movement information available in the literature. Aerogeophysical data were acquired along north-south lines spaced at 500 m, with a mean terrain clearance of 100 m. These data cover potassium (K, %), equivalent in thorium (eTh, ppm) and equivalent in uranium (eU, ppm). After performing a critical analysis of the data, basic (K, eU, eTh) and ternary (R-K/G-eTh/B-eU) maps were generated and then superimposed on the digital elevation model (DEM). The investigation of the radionuclide mobility across the relief and weathering mantle consisted of an analysis of the schematic profiles of elevation related with each radionuclide; a comparison of the K, eU and eTh maps with their 3D correspondents; and the study of mass movements registered in the region. A statistical comparison of lithogeochemical (K, U, Th) and geophysical (K, eU, eTh) data showed consistency in all the granitic suites studied (Morro Inglês, Rio do Poço and Canavieiras-Estrela). Through gamma-ray spectrometry, it was possible to establish relationships between scars (from mass movements) and the gamma-ray responses as well as the radionuclide mobility and the relief and to map the granitic bodies.

  11. Family Health Program and ambulatory care-sensitive conditions in Southern Brazil.

    PubMed

    Nedel, Fúlvio Borges; Facchini, Luiz Augusto; Martín-Mateo, Miguel; Vieira, Lúcia Azambuja Saraiva; Thumé, Elaine

    2008-12-01

    Ambulatory care-sensitive conditions (ACSC) are health problems managed by actions at the first level of care. The need for hospitalization by these causes is avoidable through an effective and proper primary health care. The objective of the study was to estimate ACSC among patients hospitalized by the Sistema Unico de Saúde (Brazilian Health System). Hospital-based cross-sectional study involving 1,200 inhabitants of Bagé (Southern Brazil) who were inpatients between September/2006 and January/2007. The patients answered a questionnaire applied by interviewers and were classified according to the model of attention utilized prior to hospitalization. ACSC were defined in a workshop promoted by the Ministry of Health. The variables analyzed included demographic and socioeconomic characteristics, health and health services utilized. Multivariate analysis was conducted by the Poisson model, according to a hierarchical conceptual framework, stratified by sex and model of care. ACSC accounted for 42.6% of the hospitalizations. The probability that the main diagnosis for hospitalization is considered an ACSC is greater among women, children under five years of age, individuals with less then five years of schooling, hospitalization in the year prior to the interview, emergency room consultation, and being an inpatient at the university hospital. Among women, ACSC are associated with age, educational level, length of time the health center has been in existence, living in an area covered by the Programa Saúde da Família (Family Health Program), use of this service, emergency room consultation during the month prior to the interview and hospital to which patient was admitted. For men, it was associated with age, have undergone another hospitalization in the year prior to the interview and hospital to which patient was admitted. Analysis of ACSC allows identifying groups with inadequate access to primary health care. Although we could not infer an effect on the risk of

  12. Postnatal depression in Southern Brazil: prevalence and its demographic and socioeconomic determinants

    PubMed Central

    Tannous, Leila; Gigante, Luciana P; Fuchs, Sandra C; Busnello, Ellis DA

    2008-01-01

    Background Studies investigating the prevalence of postnatal depression (PND) show rates ranging from 5% to 36.7%. The investigation of age, race, educational levels, religion and income as risk factors for PND has yielded conflicting results. The aim of this study is to investigate the prevalence of PND in women residing in Southern Brazil and the associated risk factors. Methods This is population-based cross-sectional study of women residing in Porto Alegre who delivered in June 2001. A sample of 271 participants were selected from the Record of Living Newborn Infants of the State Health Department (the official Brazilian database and stores the name and address of all women who give birth to living newborn infants) using a process based on pseudo-random numbers which choose a random sample from 2.000 records. Once the addresses were identified, the women were visited at their place of residence (home, hotel, boarding house and prison), with the interviews taking place between the 6th and the 8th week after delivery. The association between the risk factors and PND was investigated through bivariate analysis using Pearson's chi-square test. Student's t-test was used to analyze the continuous variables. To identify independent risk factors, multivariate analysis was performed using hierarchical levels with a predefined model that took into account the time relationship between PND and the risk factors. Cox's regression was used to calculate the prevalence ratios. Results The PND prevalence rate found was 20.7% (CI 95% 15.7 – 25.7). After adjusting for confounding variables, per capita income was found to have a significant association with PND. Conclusion The prevalence of PND is higher than the figures found in most developed countries and similar to the figures found in developing countries. Differences in PND by regions or countries can be partially explained by the effect of income on the mediation of risk factors. In low income populations, women should be

  13. Soil organic carbon distribution in an agricultural catchment in Southern Brazil: from hillslope to catchment scale.

    NASA Astrophysics Data System (ADS)

    Trigalet, Sylvain; Chartin, Caroline; Van Oost, Kristof; van Wesemael, Bas

    2017-04-01

    Understanding the soil organic carbon (SOC) distribution a few decades after conversion to cropland and plantations in a hilly catchment in southern Brazil is challenging due to scale-dependent controlling factors. Firstly, SOC, bulk density (BD) and texture were measured by depth intervals along 18 soil profiles located in three topographical positions (sloping plateau, central back slope and concave foot slope) in cropland and forest with contrasting slopes. SOC stocks in concave footslope position were not significantly different between fields on steep (11.1 kg C m-2) and gentle slopes (12.8 kg C m-2). However, in eroding profiles, SOC stocks are twice as high in fields on gentle slopes (17.6/12.6 kg C m-2) compared to steep slopes (8.3/7.1 kg C m-2). SOC stocks on steep slope on cropland (8.8 kg C m-2) are three times lower than SOC stocks on steep slope under undisturbed forest (23.7 kg C m-2). On gentle slopes, the effect of deforestation on SOC stocks was not so drastic (14.3 and 14.4 kg C m-2). Therefore, contrasting topography generates different patterns of SOC redistribution in the catchment. The effect of conversion to cropland is probably due to soil redistribution by water and tillage erosion aggravated by the steep terrain. Secondly, in order to assess the heterogeneity of SOC distribution at catchment scale, samples were collected at 10-20; 40-50 and 75-85 cm in 167 soil profiles sampled with an auger. SOC concentrations (gC kg-1 ) in numerous bulk soil samples (n = 378) were predicted by VIS-NIR spectroscopy and partial least-square regression models. SOC stocks were assessed by a mass preserving spline tool by interpolating SOC mass at the three non-contiguous depth intervals. Samples of calibration-validation dataset (n = 95) were used for physical SOC fractionation allowing the measurement of carbon associated with < 20 μm fraction. Multivariate linear regression models and Pearson correlation coefficients were used to assess the influence of

  14. An agro-climatic approach to determine citrus postbloom fruit drop risk in Southern Brazil.

    PubMed

    Soares-Colletti, Ana R; Alvares, Clayton A; Sentelhas, Paulo C

    2016-06-01

    Postbloom fruit drop (PFD) causes lesions on the petals of citrus flowers and induces fruit abscission causing severe damage to production when the flowering period coincides with intense rainfall. The aims of this study were to develop a phenological-climatological model for citrus PFD occurrence and, together with weather data series from several locations, to determine and map the agro-climatic favorability of PFD occurrence in the state of São Paulo, Southern Brazil. A phenological flowering model was developed to identify when citrus flowering occurs. The flowering starts after when a temperature below 10 °C in the months of June or July is reached followed by cumulative rainfall within 5 days of at least 20 mm, and then 96 °C days. Between the beginning of flowering and its peak, 147 °C days are required, and between the peak and its end, approximately 229 °C days, being 206 °C days from the peak to the moment when flowers remaining are about 50 % of total. The relationship between PFD incidence and accumulated rainfall during the critical period (between flowering peak and 50 % of flowers remaining) was adjusted by the Gompertz model (R (2) = 0.99, p < 0.05). After its validation, this model was used to estimate PFD incidence for 29 locations in the state, from 1993 to 2013, which allowed to map the PFD climatic favorability for the state through a Geographical Information System using linear models based on latitude, longitude, and altitude. The obtained map showed a trend of PFD incidence increasing from the northwest of the state of São Paulo towards the south and the coastal region, with medium to very high favorability in the center of the state. The results of this study can be used by growers as a guide for disease control planning as well as for defining the regions where the climatic conditions are likely to escape this disease.

  15. Relative growth of Acantholobulus schmitti (Rathbun, 1930) (Crustacea, Brachyura, Panopeidae) at Guaratuba Bay, southern Brazil.

    PubMed

    Frigotto, S F; Marochi, M Z; Masunari, S

    2013-11-01

    A study on the relative growth of the crab Acantholobulus schmitti (Rathbun, 1930) (Decapoda, Brachyura, Panopeidae) was performed with allometric techniques. The species is associated with lanterns used for oyster farming at Guaratuba Bay, southern Brazil. Crabs were obtained from five lanterns from March through December 2009. The following morphological characteristics were measured: the carapace width (CW), the height and length of the major cheliped propodus (LPH and LPL), the width of the female abdomen at the base of the 4th somite (AW) and the length of the male gonopod (GL). A total of 1,004 individuals of A. schmitti were measured, of which 451 were males, 323 non-ovigerous females and 230 ovigerous females. The CW of the males varied from 1.87 to 11.86 mm, that of non-ovigerous females from 1.44 to 8.77 mm and that of ovigerous females from 4.09 to 11.12 mm. The plot of LPH against CW showed a mean inflection point at 3.67 mm CW for the males. This result indicated that male crabs are juvenile below this value and adult above it. For the females, the corresponding inflection point occurred at 3.36 mm CW in the plot of AW against CW. These results, CW x LPH and CW x AW, highlight the importance of chelae enlargement at the onset of maturation in males. Similarly, the widening of the abdomen in females is required to accommodate egg clutches during the reproductive period. Heterochely was recorded in both sexes. Right-handed crabs were more frequent than left-handed ones, with percentages of 75.8% in males and 82.7% in females. The population of A. schmitti from Guaratuba Bay becomes sexually mature at an earlier age than A. schmitti from the coast of São Paulo state. The lanterns used in oyster farming furnish a safe habitat in which these crabs can spend their entire benthic life.

  16. Seasonal variations in the biochemical composition and reproductive cycle of the ghost crab Ocypode quadrata (Fabricius, 1787) in Southern Brazil.

    PubMed

    Antunes, Graziani de Freitas; do Amaral, Ana Paula Nunes; Ribarcki, Fabiana Pinto; Wiilland, Elenir de Fátima; Zancan, Denise Maria; Vinagre, Anapaula Sommer

    2010-06-01

    The ghost crab, Ocypode quadrata, is found on sandy beaches from the United States to Southern Brazil. Because there is still little information about the metabolism and reproduction of O. quadrata on the southern coast of Brazil, the objectives of this study were to (i) evaluate the effect of seasonal variations on the carbohydrate, lipid, and protein metabolism of O. quadrata at Rondinha Beach, a beach with high anthropogenic activity, and to compare it with data from Siriú Beach, which has less human activity; and (ii) describe the effect of seasonal variations on the histological characteristics of male and female gonads, in order to assess the reproductive capacity of the crabs. The gonads of male crabs showed no significant variations in the gonadosomatic index (GSI) and glycogen levels; however, histological analysis of the testes revealed that they are mature in the summer. In females, the GSI and glycogen values are higher in summer, concomitantly with the presence of mature oocytes. These results suggest that the reproductive peak of O. quadrata occurs in summer. The seasonal analysis of the biochemical parameters, as well as comparison with Siriú Beach, demonstrated that the ghost crabs of Rondinha Beach have a different pattern of metabolism than those of Siriú. This difference may be a consequence of differences in the environmental conditions as well as in the anthropogenic pressures, such as vehicle traffic and the increase in human population at the beach in summer.

  17. Human papillomavirus type distribution and HPV16 intratype diversity in southern Brazil in women with and without cervical lesions

    PubMed Central

    de Oliveira, Gisele R; Vieira, Valdimara C; Ávila, Emiliana C; Finger-Jardim, Fabiana; Caldeira, Thaís DM; Gatti, Fabiane AA; Gonçalves, Carla V; Oliveira, Sandro G; da Hora, Vanusa P; Soares, Marcelo A; de Martinez, Ana MB

    2017-01-01

    BACKGROUND Increasing evidence suggests that human papillomavirus (HPV) intratype variants (specific lineages and sublineages) are associated with pathogenesis and progression from HPV infection to persistence and the development of cervical cancer. OBJECTIVES This study aimed to verify the prevalence of HPV infection and distribution of HPV types and HPV16 variants in southern Brazil in women with normal cytology or intraepithelial lesions. METHODS HPV typing was determined by L1 gene sequencing. To identify HPV16 variants, the LCR and E6 regions were sequenced, and characteristic single nucleotide variants were identified. FINDINGS A total of 445 samples were studied, with 355 from cervical scrapes and 90 from cervical biopsies. HPV was detected in 24% and 91% of these samples, respectively. The most prevalent HPV types observed were 16 (cervical, 24%; biopsies, 57%) and 58 (cervical, 12%; biopsies, 12%). Seventy-five percent of the HPV16-positive samples were classified into lineages, with 88% defined as lineage A, 10% as lineage D, and 2% as lineage B. MAIN CONCLUSIONS This study identified a high frequency of European and North American HPV16 lineages, consistent with the genetic background of the human population in southern Brazil. PMID:28591310

  18. HIV-related stigma, service utilization, and status disclosure among truck drivers crossing the Southern borders in Brazil.

    PubMed

    Pulerwitz, J; Michaelis, A P; Lippman, S A; Chinaglia, M; Díaz, J

    2008-08-01

    HIV-related stigma and discrimination (S&D) have been shown to impede prevention, care and treatment. Yet, few quantitative studies have tested the associations between stigma, service utilization and status disclosure, especially in countries with concentrated HIV epidemics. Surveys, administered to a random sample of 1,775 truck drivers crossing Southern borders in Brazil, included items on multiple conceptual domains of S&D, such as fear of casual contact and blame towards people living with HIV/AIDS. Pearson's chi-square tests and logistic regression were used to examine correlations. Less stigma (both individual items and grouped as a scale) was significantly correlated with VCT use (pSouthern Brazil. Learning more about stigma is important given the growing assertions that testing is a 'critical gateway' to HIV prevention and treatment. As access to HIV testing and treatment improves, providers increasingly need to understand and address how stigma acts as a barrier to services.

  19. Frequencies of polymorphisms of the Rh, Kell, Kidd, Duffy and Diego systems of Santa Catarina, Southern Brazil.

    PubMed

    Costa, Daiane Cobianchi; Schinaider, Alessandra Arruda; Santos, Thais Mattos; Schörner, Everaldo José; Simon, Daniel; Maluf, Sharbel Weidner; de Moraes, Ana Carolina Rabello; Silva, Maria Claudia Silva

    2016-01-01

    Red blood cell genes are highly polymorphic with the distribution of alleles varying between different populations and ethnic groups. The objective of this study was to investigate gene polymorphisms of blood groups in the state of Santa Catarina, Southern Brazil. Three hundred and seventy-three unrelated blood donors and 31 transfusion-dependent patients were evaluated to investigate polymorphisms of the Rh, Kell, Duffy, Kidd, and Diego blood group systems in a population from the state of Santa Catarina. The subjects, from seven regions that comprise the blood-banking network of the state, were assessed between August 2011 and March 2014. The genotypes of the Rh, Kell, Duffy, Kidd, and Diego systems were determined using the restriction fragment length polymorphism-polymerase chain reaction and allele-specific polymerase chain reaction techniques. The genotype frequencies in this study were significantly different when populations from different regions of Santa Catarina were compared. Furthermore, there were also significant differences in the genetic frequencies compared to other Brazilian states. The genotype frequencies of the Kell and Kidd blood groups are similar to European populations from Naples, Italy and Zurich, Switzerland. This article reports for the first time the frequency of polymorphisms of blood group systems in blood donors from Santa Catarina, Southern Brazil. Copyright © 2016 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. All rights reserved.

  20. Epidemiologic investigation of an occupational illness of tobacco harvesters in southern Brazil, a worldwide leader in tobacco production.

    PubMed

    Bartholomay, Patrícia; Iser, Betine Pinto Moehlecke; de Oliveira, Patrícia Pereira Vasconcelos; dos Santos, Tania Esther Herc Holmer; Malta, Deborah Carvalho; Sobel, Jeremy; de Moura, Lenildo

    2012-07-01

    As part of smoking surveillance, the authors conducted an epidemiologic investigation in southern Brazil to identify the occurrence of Green Tobacco Sickness and risk factors for illness and to recommend control and prevention measures. A 1:2 case-control study matched by subjects' smoking habits. The study population was residents of Candelária, Rio Grande do Sul state, who farm tobacco and provided a urine sample for cotinine measurement by high-performance liquid chromatography. Confirmed cases were persons with compatible clinical presentation (headache, nausea, vomit, dizziness or weakness) and cotinine level >10 ng/ml. Controls were persons without compatible signs or symptoms. The association measure was the matched OR with 95% CIs and p<0.05. Of 33 confirmed cases, 64% were men, average age was 33 years (SD ± 11.8 years) and 57% were landowners. Cases have had similar illness in the past and were likelier to be workers hired by farmers-landowners than controls. Multivariate analysis yielded independent association between these variables and illness, controlled for age and sex. Contact with pesticides and working with wet tobacco leaves were not associated with illness. The authors confirmed Green Tobacco Sickness in southern Brazil; the authors recommend investigation of its prevalence in tobacco-growing regions and monitoring of and education about the disease and its prevention by occupational health authorities.

  1. Swine Influenza Virus and Association with the Porcine Respiratory Disease Complex in Pig Farms in Southern Brazil.

    PubMed

    Schmidt, C; Cibulski, S P; Andrade, C P; Teixeira, T F; Varela, A P M; Scheffer, C M; Franco, A C; de Almeida, L L; Roehe, P M

    2016-05-01

    Despite the putative endemic status of swine influenza A virus (swIAV) infections, data on the occurrence of swine influenza outbreaks are scarce in Brazil. The aim of this study was to detect and subtype swIAVs from six outbreaks of porcine respiratory disease complex (PRDC) in southern Brazil. Nasal swabs were collected from 66 piglets with signs of respiratory disease in six herds. Lung tissue samples were collected from six necropsied animals. Virus detection was performed by PCR screening and confirmed by virus isolation and hemagglutination (HA). Influenza A subtyping was performed by a real-time reverse transcriptase PCR (rRT-PCR) to detect the A(H1N1)pdm09; other swIAV subtypes were determined by multiplex RT-PCR. In lung tissues, the major bacterial and viral pathogens associated with PRDC (Pasteurella multocida, Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, Haemophilus parasuis and PCV2) were investigated. In some affected pigs, clinico-pathological evaluations were conducted. Influenza A was detected by screening PCR in 46 of 66 swab samples and from five of six lungs. Virus was recovered from pigs of all six herds. Subtype A(H1N1)pdm09 was detected in four of six herds and H1N2 in the other two herds. In lung tissues, further agents involved in PRDC were detected in all cases; Pasteurella multocida was identified in five of six samples and Mycoplasma hyopneumoniae in three of six. Actinobacillus pleuropneumoniae (1/6), Haemophilus parasuis (1/6) and PCV2 (1/6) were also detected. These findings indicate that subtypes A(H1N1)pdm09 and H1N2 were present in pigs in southern Brazil and were associated with PRDC outbreaks.

  2. Risk factors for hepatitis C virus infection among blood donors in southern Brazil: a case-control study

    PubMed Central

    Brandão, Ajacio BM; Costa Fuchs, Sandra

    2002-01-01

    Background In Brazil, it is estimated that between 2.5 and 4.9% of the general population present anti-hepatitis C virus (HCV) antibodies, which corresponds to as many as 3.9 to 7.6 million chronic carriers. Chronic liver disease is associated with HCV infection in 20% to 58% of the Brazilian patients. The objective of this case-control study was to investigate the risk factors for presence of anti-HCV antibody in blood donors in southern Brazil. Methods One hundred and seventy eight blood donors with two positive ELISA results for anti-HCV were cases, and 356 controls tested negative. A standardized questionnaire was used to collect data concerning demographic and socioeconomic aspects, history of previous hepatitis infection, social and sexual behaviors, and number of donations. Variables were grouped into sets of hierarchical categories. Cases and controls were compared using logistic regression, odds ratios, and 95% confidence intervals. The statistical significance of the associations was assessed through likelihood ratio tests based on a P value < 0.05. Results The prevalence of anti-HCV among blood donors was 1.1%. Most of the donors were white and males. In the multivariate analysis, independent predictors of anti-HCV positivity were: intravenous drug use, blood transfusion >10 years earlier, having had two to four sexually transmitted diseases, incarceration, tattooing, sex with a hepatitis B or C virus carrier or with intravenous drug users. Conclusion Intravenous drug use, blood transfusion, and tattooing were the main risk factors for anti-HCV positivity among blood donors from southern Brazil, but sexual HCV transmission should also be considered. PMID:12169200

  3. A New Species of Paracoccus Ezzat and McConnell (Hemiptera: Pseudococcidae) from Southern Brazil.

    PubMed

    Pacheco da Silva, V C; Kaydan, M B; Germain, J-F; Botton, M

    2016-12-01

    The worldwide mealybug genus Paracoccus Ezzat & McConnell (Hemiptera: Coccomorpha: Pseudococcidae) has eight described Neotropical species, including two species known from Brazil. In this article, we describe a third species from Brazil: Paracoccus galzerae Pacheco da Silva & Kaydan sp. n., based on the morphology of adult females collected on the roots of Conyza bonariensis (Asteraceae) in vineyards in Bento Gonçalves City, Rio Grande do Sul. A revised identification key including the new species is provided for the Neotropical region.

  4. [Anthropometric indices among schoolchildren from a municipality in Southern Brazil: a descriptive analysis using the LMS method].

    PubMed

    Filho, Valter Cordeiro Barbosa; Lopes, Adair da Silva; Fagundes, Ricardo Rosa; de Campos, Wagner

    2014-12-01

    To describe the percentile values for body mass index (BMI), waist circumference (WC) and waist-to-height (WHtR) of children from Colombo, Brazil, and compare them with data of children from other countries. This was a cross-sectional study with a random sample of 2,035 children aged 6-11 years. Age-and sex-specific smoothed percentiles curves for BMI, WC and WHtR were created using the LMS method. Values of 10(th), 50(th) and 90(th) percentiles from Brazilian children were compared with data from other countries. There was a trend of increasing BMI and WC with age in both sexes. WHtR remained constant with advancing age in boys and girls. Comparison of the growth pattern among countries showed clear differences. Southern Brazil boys and girls had elevated 90(th) percentile values for BMI, which was similar to German children and higher than the North American and World Health Organization percentile values. However, children from this study had intermediate values for WC and WHtR in comparison to children from other countries. Elevated BMI values were observed among southern Brazilian children, but WC and WHtR percentile values were lower in southern Brazilian children than in children from other countries. Interventions at different levels should be made to avoid a probable increase of nutritional disorders (especially general obesity) in the next years. Copyright © 2014 Associação de Pediatria de São Paulo. Publicado por Elsevier Editora Ltda. All rights reserved.

  5. Does the Lagoa do peixe sandbar opening influence the macrophyte richness and composition in Southern Brazil wetlands?

    PubMed

    Rolon, Ana Silvia; Rocha, Odete; Maltchik, Leonardo

    2013-03-01

    The Lagoa do Peixe has its connection with the sea artificially opened every year at the end of winter. However, this management has been carried out without the evaluation of the impact of this opening in the aquatic biodiversity. This information is crucial for the management of the natural resources of the Lagoa do Peixe National Park, the unique Ramsar site in Southern Brazil. The following questions were analyzed: (1) Do richness and composition of aquatic macrophytes from Lagoa do Peixe floodplain varies temporarily according to the sandbar opening and closing? (2) Does the variation pattern of the macrophyte community changed according to the sandbar opening and closing? A set of eight sampling sites of 1ha were selected over the Lagoa do Peixe floodplain: four sites not influenced by the artificial sandbar opening and four sites influenced by this event, being two sites closer to the sandbar opening and the two sites distant to the sandbar opening. The samplings were carried out between November 2007 and October 2009. The results show that although the artificial sandbar breaching does not affect the aquatic macrophyte richness at the floodplain, it affects the dynamics of species composition. The hydrological variation related to this management can be the main factor of the continuous change in the species composition in the floodplain, especially in the Southern portion. In order to avoid impacts in the macrophyte conservation, the artificial sandbar opening should be considered carefully, since the area of study is one of the most important conservation units to wetland systems in Southern Brazil.

  6. Spider (Arachnida, Araneae) diversity in secondary and old-growth southern Atlantic forests of Paraná state, Brazil.

    PubMed

    Raub, Florian; Höfer, Hubert; Scheuermann, Ludger

    2017-07-01

    The data presented here have been collected in the southern part of the Atlantic Forest (Mata Atlântica) in the state of Paraná, Brazil within a bilateral scientific project (SOLOBIOMA). The project aimed to assess the quality of secondary forests of different regeneration stages in comparison with old-growth forests with regard to diversity of soil animals and related functions. The Atlantic Forest is a hotspot of biological diversity with an exceptionally high degree of endemic species, extending over a range of 3,500 km along the coast of Brazil. The anthropogenic pressure in the region is very high with three of the biggest cities of Brazil (São Paulo, Rio de Janeiro, and Curitiba) lying in its extension. An evaluation of the value of secondary forests for biodiversity conservation is becoming more and more important due to the complete disappearance of primary forests. In 2005, we sampled spiders in 12 sites of three successional stages (5-8, 10-15, 35-50 yr old, three replicates of each forest stage) and old-growth forests (> 100 yr untouched, also three replicates). All sites were inside a private nature reserve (Rio Cachoeira Nature Reserve). We repeated the sampling design and procedure in 2007 in a second private reserve (Itaqui Nature Reserve). The two nature reserves are within about 25 km of each other within a well preserved region of the Mata Atlântica, where the matrix of the landscape mosaic is still forest. A widely accepted standard protocol was used in a replicated sampling design to apply statistical analyses to the resulting data set and allow for comparison with other studies in Brazil. Spiders were sorted to family level and counted; the adult spiders further identified to species if possible or classified as morphospecies with the help of several spider specialists. © 2017 by the Ecological Society of America.

  7. Magma-assisted strain localization in an orogen-parallel transcurrent shear zone of southern Brazil

    NASA Astrophysics Data System (ADS)

    Tommasi, AndréA.; Vauchez, Alain; Femandes, Luis A. D.; Porcher, Carla C.

    1994-04-01

    In a lithospheric-scale, orogen-parallel transcurrent shear zone of the Pan-African Dom Feliciano belt of southern Brazil, two successive generations of magmas, an early calc-alkaline and a late peraluminous, have been emplaced during deformation. Microstructures show that these granitoids experienced a progressive deformation from magmatic to solid state under decreasing temperature conditions. Magmatic deformation is indicated by the coexistence of aligned K-feldspar, plagioclase, micas, and/or tourmaline with undeformed quartz. Submagmatic deformation is characterized by strain features, such as fractures, lattice bending, or replacement reactions affecting only the early crystallized phases. High-temperature solid-state deformation is characterized by extensive grain boundary migration in quartz, myrmekitic K-feldspar replacement, and dynamic recrystallization of both K-feldspar and plagioclase. Decreasing temperature during solid-state deformation is inferred from changes in quartz crystallographic fabrics, decrease in grain size of recrystallized feldspars, and lower Ti amount in recrystallized biotites. Final low-temperature deformation is characterized by feldspar replacement by micas. The geochemical evolution of the synkinematic magmatism, from calc-alkaline metaluminous granodiorites with intermediate 87Sr/86Sr initial ratio to peraluminous granites with very high 87Sr/86Sr initial ratio, suggests an early lower crustal source or a mixed mantle/crustal source, followed by a middle to upper crustal source for the melts. Shearing in lithospheric faults may induce partial melting in the lower crust by shear heating in the upper mantle, but, whatever the process initiating partial melting, lithospheric transcurrent shear zones may collect melt at different depths. Because they enhance the vertical permeability of the crust, these zones may then act as heat conductors (by advection), promoting an upward propagation of partial melting in the crust

  8. Phytolith studies applied to geomorphologic analysis in the Southern Espinhaço Mountain Range, Brazil

    NASA Astrophysics Data System (ADS)

    Coe, H. H.; Augustin, C. R.; Chueng, K. F.

    2012-12-01

    Phytoliths are particles of silica formed as a result of absorption of silicic acid in the soil solution by plant roots and then precipitate in plant cells. They enable paleoenvironmental reconstruction, indicating climate change and its effects on vegetation, offering clues about factors that may influence geomorphologic processes. Samples were collected at the Serra do Engenho, part of the Southern Espinhaço, Minas Gerais, Brazil, in two profiles formed by sandy soils located in a slightly inclined slope. Profile 1 shows a slightly concavity and an abrupt break of slope in its contact with a quartzite outcrop. It is located at the upper portion of the slope and presents a thick layer of material in which it is possible to identify depositional sequences. This layer although thinner is also present at the profile 2, located at the middle slope. There are evidences that the deposition of these sequences have occurred at the same time, but the type or the intensity of the accumulative processes have occurred in different ways, as indicated by phytoliths and others. Both profiles present a detrictic pavement at the same depth (50-60 cm), on the top of which in P2 occurs an organic horizon. In both profiles and in almost all modern vegetation assemblages collected near them, phytoliths are very weathered. This makes identification of the types and calculation of phytolith indexes difficult, but highlights the intensity of erosion in the area. The results also show the dominance of big phytoliths, because they are more resistant to erosion, and low proportions of short cells phytoliths, more fragile ones. Organic carbon stocks are higher in P2 than in P1, due to the presence of the organic horizon, formed because of better conditions of accumulation and decomposition of organic matter. It does not occur in P1, where the runoff until nowadays is more effective due to the proximity of the outcrop and to its small presence of lower strata of vegetation cover. These

  9. Brazil.

    PubMed

    1983-07-01

    Attention in this discussion of Brazil focuses: the history of the country's demographic situation; government's overall approach to population problems; population data systems and development planning; institutional arrangements for the integration of population within development planning; government's view of the importance of population policy in achieving development objectives; population size, growth, and natural increase; fertility; international migration; and spatial distribution. The population of Brazil grew from 17 million in 1900 to about 119 million in 1960, making it the most populous country in the world and 1 of the relatively few countries to have sustained rates of population growth of more than 2% for over a century. The government has not adopted an explicit policy to modify fertility or population growth. Initially this was because of its positive perception of the benefits of population growth and a large population size and, amore recently, because of Brazil's gradual transition to more moderate levels of fertility and population growth. Brazil's main sources of demographic data are its 9 censuses, conducted in 1982, 1890, 1900, 1920, 1940, 1950, 1960, 1970, and most recently in August 1980. A nationwide system of vital registration data are still lacking in many geographic areas, researchers have had to rely on indirect estimation techniques to derive estimates of past trends in fertility and mortality. Population policy has been regarded as a highly sensitive issue by Brazilian officials, and the government remains cautious in regard to population issues. Preliminary results of Brazil's 1980 census indicate a population of 119 million and an annual rate of population growth of 2.1%, continuing the downward trend that was first evident in 1976. The government considers levels and trends of population growth to be satisfactory, and morbidity and mortality to be unacceptable, partly because of a lack of success in reducing the incidence of

  10. Visiting bees of cucurbita flowers (cucurbitaceae) with emphasis on the presence of peponapis fervens smith (eucerini - apidae) - Santa Catarina, Southern Brazil.

    USDA-ARS?s Scientific Manuscript database

    Cucurbita flowers are monoecious, the male and female requiring a pollinator to transfer pollen. Bees were systematically collected as they visited flowers of three cultivated Cucurbita species grown at seven separate localities of Santa Catarina state in southern Brazil. Additionally, pantraps we...

  11. Epidemiological and biological aspects on Ornithodoros brasiliensis (mouro tick), an argasidae tick only found on the highlands region of Rio Grande do Sul state, southern Brazil

    USDA-ARS?s Scientific Manuscript database

    The soft tick Ornithodoros brasiliensis (Acari: Argasidae) is present in farms along the highlands of Rio Grande do Sul state in southern Brazil. Reports of human parasitism by O. brasiliensis drew the attention of local health authorities. A preliminary epidemiological survey was conducted to ident...

  12. Molecular and Morphological Evidence Reveals a New Species in the Phyllomedusa hypochondrialis Group (Hylidae, Phyllomedusinae) from the Atlantic Forest of the Highlands of Southern Brazil

    PubMed Central

    Bruschi, Daniel P.; Lucas, Elaine M.; Garcia, Paulo C. A.; Recco-Pimentel, Shirlei M.

    2014-01-01

    The taxonomic status of a disjunctive population of Phyllomedusa from southern Brazil was diagnosed using molecular, chromosomal, and morphological approaches, which resulted in the recognition of a new species of the P. hypochondrialis group. Here, we describe P. rustica sp. n. from the Atlantic Forest biome, found in natural highland grassland formations on a plateau in the south of Brazil. Phylogenetic inferences placed P. rustica sp. n. in a subclade that includes P. rhodei + all the highland species of the clade. Chromosomal morphology is conservative, supporting the inference of homologies among the karyotypes of the species of this genus. Phyllomedusa rustica is apparently restricted to its type-locality, and we discuss the potential impact on the strategies applied to the conservation of the natural grassland formations found within the Brazilian Atlantic Forest biome in southern Brazil. We suggest that conservation strategies should be modified to guarantee the preservation of this species. PMID:25141279

  13. Assessment of Yield with Altered Soybean Traits Under Climate Change in Southern Brazil: Performance of the Crop Model and Genotype Trait

    NASA Astrophysics Data System (ADS)

    Battisti, R.; Sentelhas, P. C.; Boote, K. J.

    2014-12-01

    Water deficit is the main factor responsible for soybean yield gap in Southern Brazil and tends to increase under climate change scenarios. Alternatives need be identified in order to create options to reduce the yield gap. One alternative is the selection of soybean cultivars with traits associated to drought tolerance. Thus, the aim of this study, via use of crop models, is to evaluate genetic traits that can help to improve soybean yield under climate change scenarios in Southern Brazil by improving the crop tolerance to water deficit. For this, the CSM-CROPGRO-Soybean model was used to simulate soybean yield in Southern Brazil and evaluate yield with altered genetic traits. The first step was to calibrate the model for a Brazilian soybean cultivar, using results from irrigated and rainfed experiments conducted in different sowing dates and locations in Southern Brazil. The CSM-CROPGRO-Soybean estimated a mean yield of 3551 (SD = 1439) kg ha-1, while the mean observed yield was 3313 (SD = 1105) kg ha-1. The mean error was 238 kg ha-1 (7.2%), mean error absolute was 765 kg ha-1 (23%) and modeling efficiency was 0.27, with a good relationship between observed and simulated yield (Figure 1). The model was able to estimate soybean yield for different levels of soil water available during the crop season. The next steps of the project are to manipulate the coefficients related with rooting depth extension, early stomata closure at high soil water contents, transpiration limited to a maximum rate, and N2 fixation drought tolerance in the model in order to identify their impact on yield under current and future climate scenarios in all soybean production regions in Southern Brazil. The soybean yield from different changed traits will be evaluated in order to define guidelines for breeding programs, allowing the saving of resources and time in the new cultivars development process, focused on adaptation to climate change.

  14. Risk Factors for Death from Pandemic (H1N1) 2009, Southern Brazil

    PubMed Central

    Skalinski, Lacita M.; Igansi, Cristine N.; de Souza, Libia R.O.; Iser, Betine P.M.; Reis, Priscilleyne O.; Barros, Eliana N.C.; Macário, Eduardo M.; Bercini, Marilina A.; Ranieri, Tani M.S.; Araújo, Wildo N.

    2011-01-01

    To identify risk factors for death from pandemic (H1N1) 2009, we obtained data for 157 hospitalized patients with confirmed cases of this disease. Multivariate analysis showed that diabetes and class III obesity were associated with death. These findings helped define priority vaccination groups in Brazil. PMID:21801625

  15. Risk factors for death from pandemic (H1N1) 2009, southern Brazil.

    PubMed

    Yokota, Renata T C; Skalinski, Lacita M; Igansi, Cristine N; de Souza, Libia R O; Iser, Betine P M; Reis, Priscilleyne O; Barros, Eliana N C; Macário, Eduardo M; Bercini, Marilina A; Ranieri, Tani M S; Araújo, Wildo N

    2011-08-01

    To identify risk factors for death from pandemic (H1N1) 2009, we obtained data for 157 hospitalized patients with confirmed cases of this disease. Multivariate analysis showed that diabetes and class III obesity were associated with death. These findings helped define priority vaccination groups in Brazil.

  16. Wave Attenuation on Muddy Bottoms - A Multidisciplinary Field Study Offshore Cassino Beach, Southern Brazil

    DTIC Science & Technology

    2005-09-30

    University of Rio de Janeiro Ocean Engineering Program / COPPE CP 68508 - Centro de Tecnologia - C203 21945-970 Rio de Janeiro, Brazil Phone/fax...NAME(S) AND ADDRESS(ES) Federal University of Rio de Janeiro,Ocean Engineering Program / COPPE, CP 68508 - Centro de Tecnologia - C203,21945-970 Rio

  17. Herpes virus inhibitory substances from Hypericum connatum Lam., a plant used in southern Brazil to treat oral lesions.

    PubMed

    Fritz, Daniela; Venturi, Caroline Rita; Cargnin, Simone; Schripsema, Jan; Roehe, Paulo Michel; Montanha, Jarbas Alves; von Poser, Gilsane Lino

    2007-09-25

    Hypericum connatum (Guttiferae) is used in southern Brazil in the treatment of lesions in the mouth, often related to acute herpetic gingivo-stomatitis. The chemical investigation of the plant revealed the presence of phloroglucinol derivatives and flavonoids. From the n-hexane extract of the aerial parts a phloroglucinol derivative, hyperbrasilol B, was isolated, while the methanolic extract afforded four flavonoids: amentoflavone, hyperoside, guaijaverine and luteoforol. The crude methanolic extract and fractions (n-hexane, dichloromethane and methanol) as well as the isolated compounds were tested for antiviral activity against herpes simplex viruses (HSV). Among the tested samples, luteoforol was the most active inhibiting the cytopathic effect (CPE) and reducing the viral titer of HSV-1 DNA viral strains KOS and VR733 (ATCC).

  18. Baseline levels of oxidative stress biomarkers in species from a subtropical estuarine system (Paranaguá Bay, southern Brazil).

    PubMed

    Sardi, Adriana E; Renaud, Paul E; da Cunha Lana, Paulo; Camus, Lionel

    2016-12-15

    Offshore petroleum exploration has increased the risks of oil spills in coastal tropical and subtropical habitats. Monitoring tools are needed to assess and protect environmental health. We determined baseline values of antioxidant biomarkers (CAT, SOD, GPx, GST, MDA) for five ecologically relevant species in a subtropical system in southern Brazil. Regional baseline levels are compared with literature data as a basis to eventually test their efficacy as post-spill monitoring tools. Differences in the antioxidant response among species, contamination, and seasons were tested using univariate and multivariate analyses. The bivalves Anomalocardia flexuosa and Crassostrea rhizophorae and the catfish Genidens genidens emerge as suitable sentinel species. Seasonality is the main factor accounting for biomarkers variability, and not background contamination level. However, interactions between season and contamination level are also significant, indicating that biomarkers respond to complex environmental settings, a fact that needs to be fully understood for designing proper monitoring programs. Copyright © 2016 Elsevier Ltd. All rights reserved.

  19. HIV/AIDS knowledge and health-related attitudes and behaviors among deaf and hearing adolescents in southern Brazil.

    PubMed

    Bisol, Cláudia Alquati; Sperb, Tania Mara; Brewer, Toye H; Kato, Sérgio Kakuta; Shor-Posner, Gail

    2008-01-01

    HIV/AIDS knowledge and health-related attitudes and behaviors among deaf and hearing adolescents in southern Brazil are described. Forty-two deaf students attending a special nonresidential public school for the deaf and 50 hearing students attending a regular public school, ages 15-21 years, answered a computer-assisted questionnaire. (There was simultaneous video translation of questions to Brazilian Sign Language.) A branched decision-tree structure was used to determine level of sexual experience and hearing status. Deaf participants scored lower on HIV/AIDS knowledge, demonstrating a need to improve school-based instruction and develop campaigns tailored to this group's requirements. Though the hearing students reported more sexual activity than the deaf students, no other significant differences were found in health-related attitudes and behaviors. Two findings of concern are the high rate of sexual abuse reported by deaf participants and the large number of deaf adolescents reporting having a friend with AIDS.

  20. Assessment of tributyltin contamination based on imposex in Stramonita rustica (Mollusca: Gastropoda) along southern Bahia coast, northeastern Brazil.

    PubMed

    Zeidan, G C; Boehs, G

    2017-03-01

    This study investigated the effects of tributyltin (TBT) on the morphology of the genital system of the gastropod Stramonita rustica in southern Bahia, Brazil. For this, 330 specimens were collected during the summer of 2014 at eight sampling points to ascertain whether male sex organs had developed in addition to the complete female genital tract in females (= imposex). The analyses were made under a stereoscopic microscope. Imposex and their associated indexes, and the sterile females, exhibited the highest rates in harbors and shipyards areas. Despite the total ban of TBT in anti-fouling paints on a global scale since 2003, the results of this and other studies indicate the continued use of those paints on the Brazilian coast. This shows the inefficiency of existing legislation and the need to strengthen enforcement of the ban.

  1. Seasonal and organ variations in antioxidant capacity, detoxifying competence and oxidative damage in freshwater and estuarine fishes from Southern Brazil.

    PubMed

    Da Rocha, A M; Salomão de Freitas, D P; Burns, M; Vieira, J P; de la Torre, F R; Monserrat, J M

    2009-11-01

    This study analyzed biochemical biomarkers of freshwater and estuarine fish species from Southern Brazil. It analyzed three organs (muscle, liver and gills), in four fish species (Micropogonias furnieri, Pimelodus pintado, Loricariichthys anus and Parapimelodus nigribarbis) in order to perform an environmental diagnosis. Obtained results showed that liver of L. anus and gills of M. furnieri presented higher total antioxidant capacity against peroxyl radicals during fall, whereas a clear seasonality was found for gill reduced glutathione (GSH) levels of all studied species, with higher concentration during spring. In terms of oxidative damage (TBARS), liver of M. furnieri and gills of P. nigribarbis showed higher TBARS levels during fall, whereas P. pintado showed the lowest TBARS value. Finally, a conspicuous seasonal effect was observed for purified and non-purified glutathione-S-transferase (GST), where minimum values were registered during fall, pointing to this season as one where fish species are less competent to perform detoxifying reactions.

  2. Diversity of culturable Gram-negative bacteria isolated from irrigation water of two rice crop regions in Southern Brazil.

    PubMed

    Reche, Maria Helena Lima Ribeiro; Reali, Catiusca; Pittol, Michele; de Athayde Saul, Danilo; Macedo, Vera Regina Mussoi; Valiati, Victor Hugo; Machado, Vilmar; Fiuza, Lidia Mariana

    2016-06-01

    In this study, we assessed the diversity of Gram-negative bacteria found in water used for irrigation of rice crops in two growing areas of southern Brazil. Samples were collected from the main irrigation channel and field drain area. Twenty-two bacterial species were found in Cachoeirinha and 28 in Camaquã. In both areas, the most frequent bacterial families were Enterobacteriaceae and Aeromonadaceae. Differences in microbial diversity were observed in both study areas. Thirty-five Gram-negative species were identified; however, only 15 were common in both locations. In addition, there were found pathogenic and drug-resistant species, such as Acinetobacter sp., Brucella spp., and Chryseobacterium meningosepticum. This study demonstrates the existence of a number of pathogenic species in aquatic ecosystems analyzed in three consecutive crop years, especially water used for rice production.

  3. The high prevalence of Torque teno virus DNA in blood donors and haemodialysis patients in southern Brazil.

    PubMed

    Massaú, Aline; Martins, Cristiana; Nachtigal, Gilca Costa; Araújo, Anelise Bergmann; Rossetti, Maria Lucia; Niel, Christian; da Silva, Cláudia Maria Dornelles

    2012-08-01

    This study investigates the frequency of Torque teno virus (TTV) infection in 150 blood donors and 77 patients requiring haemodialysis in southern Brazil. Plasma samples were screened for TTV DNA using polymerase chain reaction (PCR). The prevalences of TTV among blood donors and patients requiring haemodialysis were 73.3% and 68.8%, respectively. The presence of TTV was correlated with age in the blood donors (p = 0.024). In haemodialysis patients, no association was found between TTV infection and the demographic parameters (age, sex and education), the duration of haemodialysis or a history of blood transfusion. This study is the first to evaluate the prevalence of TTV infection in Brazilian patients requiring haemodialysis.

  4. A new species of Diomedenema (Nematoda, Rhabditida, Spiruromorpha) from Spheniscus magellanicus (Aves, Sphenisciformes) found on the southern coast of Brazil.

    PubMed

    Knoff, M; Santos, J N; Giese, E G; Gomes, D C; Silva-Souza, Â T

    2017-08-07

    A new species of the genus Diomedenema, a spiruromorph nematode, collected from the lung of Spheniscus magellanicus (Sphenisciformes) found on the southern coast of the state of Rio Grande do Sul, Brazil, is described. The new species is differentiated from the only previously described species of the genus, D. diomedeae Johston & Mawson, 1952, by males possessing a set of caudal papillae with three pairs of precloacal, two pairs of adcloacal and one pair of postcloacal papillae; precloacal papillae with the papillae of the first two pairs being closer to each other than those of the third pair; a longer and pointed tail in males; and females with the vulva at mid-body. This is the first report of a nematode infecting the lung of a sphenisciforme host.

  5. Three New Species of Phytotelm-Breeding Melanophryniscus from the Atlantic Rainforest of Southern Brazil (Anura: Bufonidae).

    PubMed

    Bornschein, Marcos R; Firkowski, Carina R; Baldo, Diego; Ribeiro, Luiz F; Belmonte-Lopes, Ricardo; Corrêa, Leandro; Morato, Sérgio A A; Pie, Marcio R

    2015-01-01

    Three new species of Melanophryniscus are described from the Serra do Mar mountain range of the state of Santa Catarina, southern Brazil. All species are found at intermediate to high altitudes and share phytotelm-breeding as their reproductive strategy. The new species are distinguished from other phytotelm-breeding Melanophryniscus based on different combinations of the following traits: snout-vent length, presence of white and/or yellow spots on forearms, mouth, belly and cloaca, pattern and arrangement of warts, and presence and number of corneous spines. The discovery of these species in a rather restricted geographical area suggests that the diversity of phytotelm-breeding species of Melanophryniscus might be severely underestimated. The conservation status of these species is of particular concern, given that one of them is at risk of extinction not only due to its restricted habitat, but also because of anthropogenic disturbances.

  6. Description of Epistylis riograndensis n.sp. (Ciliophora: Peritrichia) found in an artificial lake in Southern Brazil.

    PubMed

    Utz, Laura R P; Farias, Ana Carolina Silva Rodrigues; Freitas, Eduarda Correa; de Araújo, Gabriella Oliveira

    2014-10-03

    Epistylis riograndensis n. sp., a freshwater peritrich hosting symbiotic algae in its cytoplasm, was collected from an artificial lake, in a Botanical garden in Southern Brazil. Its detailed morphology was investigated using live and silver-stained specimens. The colonial sessile E. riograndensis has elongate zooids measuring, on average, 162 μm in length and 45 μm in width. A single contractile vacuole located near the infundibulum and a C-shaped macronucleus located transversely in the adoral half of the cell were also observed. The oral infraciliature revealed in silver-stained specimens was typical of peritrich ciliates. Three infundibular polykineties consisting of 3 rows of kinetosomes were observed. Molecular analyses of 18s rDNA placed E. riograndensis among other Epistylis species in the Order Vorticellida.

  7. Three New Species of Phytotelm-Breeding Melanophryniscus from the Atlantic Rainforest of Southern Brazil (Anura: Bufonidae)

    PubMed Central

    Bornschein, Marcos R.; Firkowski, Carina R.; Baldo, Diego; Ribeiro, Luiz F.; Belmonte-Lopes, Ricardo; Corrêa, Leandro; Morato, Sérgio A. A.; Pie, Marcio R.

    2015-01-01

    Three new species of Melanophryniscus are described from the Serra do Mar mountain range of the state of Santa Catarina, southern Brazil. All species are found at intermediate to high altitudes and share phytotelm-breeding as their reproductive strategy. The new species are distinguished from other phytotelm-breeding Melanophryniscus based on different combinations of the following traits: snout-vent length, presence of white and/or yellow spots on forearms, mouth, belly and cloaca, pattern and arrangement of warts, and presence and number of corneous spines. The discovery of these species in a rather restricted geographical area suggests that the diversity of phytotelm-breeding species of Melanophryniscus might be severely underestimated. The conservation status of these species is of particular concern, given that one of them is at risk of extinction not only due to its restricted habitat, but also because of anthropogenic disturbances. PMID:26630281

  8. Sympatric and allopatric combinations of Lymnaea columella and Fasciola hepatica from southern and south-eastern Brazil.

    PubMed

    Coelho, L H L; Lima, W S; Guimaraes, M P

    2009-09-01

    Experimental infections of Lymnaea columella with Fasciola hepatica were carried out to test the effect of sympatric and allopatric combinations between parasite and host, by using snails and flukes from southern and south-eastern Brazil. Four groups of 50 snails were infected with four miracidia per snail: two groups in sympatric and two groups in allopatric combinations. Sympatric combinations between parasite and host were more efficient than allopatric ones when snails from Itajubá were used, but the opposite was observed in infections involving snails from Pelotas. The sympatric association between L. columella and F. hepatica from Itajubá was significantly higher than in the other combinations. We concluded that the host-parasite relationship between L. columnella and F. hepatica may vary according to the geographical origin of the snails and flukes involved.

  9. Maternal age and low birth weight: a reinterpretation of their association under a demographic transition in southern Brazil.

    PubMed

    da Silva, C Homrich; Hernandez, A R; Agranonik, M; Goldani, M Z

    2013-04-01

    To evaluate the relationship between changes in fecundity rates and maternal age and the impact of maternal age on low birth weight (LBW) rates in a developed region in southern Brazil. A time series study evaluating birth weight and maternal ages through the born alive information system (SINASC) in Porto Alegre from 1996 to 2008. The Chi-square test for trends was used to evaluate the trend of LBW and fecundity rates at each maternal age. Population attributed risk (PAR) was used to calculate the impact of maternal age on LBW rates. The study included 271,100 newborns. There was a significant reduction in fecundity rates in all age groups younger than 34 years, but especially in the groups between 20 and 29 years. Overall LBW increased from 9.3 to 10.7 % (P < 0.001). The PAR for LBW showed a reduction in the group from 17 to 19 years (from 1.7 % in 1996-1999 to 0.1 % in 2004-2008), and an increase in the groups from 35 to 39 years (from 2.0 % in 1996-1999 to 2.3 % in 2004-2008) and above 40 (from 1.1 % in 1996-1999 to 1.5 % in 2004-2008). There was a significant change in fecundity pattern in the last 12 years in southern Brazil. Adolescent mothers were surpassed by mothers over 30 years of age in terms of vulnerability for LBW babies. The results show a change in the maternal age distribution towards older mothers, accompanied by an increasing incidence of LBW. This demographic transition also involved a paradoxical pattern with a remarkable reduction in fecundity rates in intermediate maternal age groups with concomitant increase in their risk for LBW.

  10. HIV behind bars: human immunodeficiency virus cluster analysis and drug resistance in a reference correctional unit from southern Brazil.

    PubMed

    Prellwitz, Isabel M; Alves, Brunna M; Ikeda, Maria Letícia R; Kuhleis, Daniele; Picon, Pedro D; Jarczewski, Carla A; Osório, Marta R; Sánchez, Alexandra; Seuánez, Héctor N; Larouzé, Bernard; Soares, Marcelo A; Soares, Esmeralda A

    2013-01-01

    People deprived of liberty in prisons are at higher risk of infection by the human immunodeficiency virus (HIV) due to their increased exposure through intravenous drug use, unprotected sexual activity, tattooing in prison and blood exposure in fights and rebellions. Yet, the contribution of intramural HIV transmission to the epidemic is scarcely known, especially in low- and middle-income settings. In this study, we surveyed 1,667 inmates incarcerated at Presídio Central de Porto Alegre, located in southern Brazil, for HIV infection and molecular characterization. The HIV seroprevalence was 6.6% (110/1,667). Further analyses were carried out on 40 HIV-seropositive inmates to assess HIV transmission clusters and drug resistance within the facility with the use of molecular and phylogenetic techniques. The molecular epidemiology of HIV-1 subtypes observed was similar to the one reported for the general population in southern Brazil, with the predominance of HIV-1 subtypes C, B, CRF31_BC and unique BC recombinants. In particular, the high rate (24%) of URF_BC found here may reflect multiple exposures of the population investigated to HIV infection. We failed to find HIV-infected inmates sharing transmission clusters with each other. Importantly, the analysis of HIV-1 pol genomic fragments evidenced high rates of HIV primary and secondary (acquired) drug resistance and an alarming proportion of virologic failure among patients under treatment, unveiling suboptimal access to antiretroviral therapy (ARV), low ARV adherence and dissemination of drug resistant HIV strains in primary infections. Our results call for immediate actions of public authority to implement preventive measures, serological screening and, for HIV-seropositive subjects, clinical and treatment follow-up in order to control HIV infection and limit the spread of drug resistance strains in Brazilian prisons.

  11. HIV behind Bars: Human Immunodeficiency Virus Cluster Analysis and Drug Resistance in a Reference Correctional Unit from Southern Brazil

    PubMed Central

    Ikeda, Maria Letícia R.; Kuhleis, Daniele; Picon, Pedro D.; Jarczewski, Carla A.; Osório, Marta R.; Sánchez, Alexandra; Seuánez, Héctor N.; Larouzé, Bernard; Soares, Marcelo A.; Soares, Esmeralda A.

    2013-01-01

    People deprived of liberty in prisons are at higher risk of infection by the human immunodeficiency virus (HIV) due to their increased exposure through intravenous drug use, unprotected sexual activity, tattooing in prison and blood exposure in fights and rebellions. Yet, the contribution of intramural HIV transmission to the epidemic is scarcely known, especially in low- and middle-income settings. In this study, we surveyed 1,667 inmates incarcerated at Presídio Central de Porto Alegre, located in southern Brazil, for HIV infection and molecular characterization. The HIV seroprevalence was 6.6% (110/1,667). Further analyses were carried out on 40 HIV-seropositive inmates to assess HIV transmission clusters and drug resistance within the facility with the use of molecular and phylogenetic techniques. The molecular epidemiology of HIV-1 subtypes observed was similar to the one reported for the general population in southern Brazil, with the predominance of HIV-1 subtypes C, B, CRF31_BC and unique BC recombinants. In particular, the high rate (24%) of URF_BC found here may reflect multiple exposures of the population investigated to HIV infection. We failed to find HIV-infected inmates sharing transmission clusters with each other. Importantly, the analysis of HIV-1 pol genomic fragments evidenced high rates of HIV primary and secondary (acquired) drug resistance and an alarming proportion of virologic failure among patients under treatment, unveiling suboptimal access to antiretroviral therapy (ARV), low ARV adherence and dissemination of drug resistant HIV strains in primary infections. Our results call for immediate actions of public authority to implement preventive measures, serological screening and, for HIV-seropositive subjects, clinical and treatment follow-up in order to control HIV infection and limit the spread of drug resistance strains in Brazilian prisons. PMID:23874857

  12. Spread of a Major Clone of Salmonella enterica Serotype Enteritidis in Poultry and in Salmonellosis Outbreaks in Southern Brazil.

    PubMed

    Borges, Karen Apellanis; Furian, Thales Quedi; de Souza, Sara Neves; Tondo, Eduardo César; Streck, André Felipe; Salle, Carlos Tadeu Pippi; de Souza Moraes, Hamilton Luiz; do Nascimento, Vladimir Pinheiro

    2017-01-01

    Salmonella spp. are among the most important agents of foodborne diseases all over the world. Human Salmonella outbreaks are often associated with the consumption of poultry products (meat and eggs), and one of the most prevalent serotypes associated with these products is Salmonella Enteritidis. Brazil is one of the most important poultry exporters in the world. In southern Brazil, three closely related clones of Salmonella Enteritidis have been responsible for the majority of foodborne Salmonella outbreaks over the past decade. However, until now, there has been little information regarding the clonal relationship among the Brazilian Salmonella strains of avian origin and those involved in foodborne outbreaks. Therefore, the aim of the present study was to complete the molecular characterization of Salmonella Enteritidis strains isolated from poultry and food sources involved in Salmonella outbreaks. PCR ribotyping was performed to discriminate the strains into different ribotype profiles according to the banding pattern amplification. This technique was able to differentiate the Salmonella Enteritidis strains into two banding patterns: R2 and R4. R2 accounted for 98.7% of the strains. DNA sequencing of the 600-bp fragment, present in all ribotypes, was applied to confirm this result. The sequences generated showed high levels of similarity, ranging from 99.7 to 100%, and were grouped into a single cluster. These results suggest that there is a clonal relationship among the Salmonella Enteritidis strains responsible for several salmonellosis outbreaks and the strains collected from poultry sources.

  13. A new prozostrodontian cynodont (Therapsida) from the Late Triassic Riograndia Assemblage Zone (Santa Maria Supersequence) of Southern Brazil.

    PubMed

    Soares, Marina B; Martinelli, Agustín G; De Oliveira, Téo V

    2014-12-01

    We report here on a new prozostrodontian cynodont, Botucaraitherium belarminoi gen. et sp. nov., from the Late Triassic Riograndia Assemblage Zone (AZ) of the Candelária Sequence (Santa Maria Supersequence), collected in the Botucaraí Hill Site, Candelária Municipality, state of Rio Grande do Sul, Brazil. The new taxon is based on a single specimen (holotype MMACR-PV-003-T) which includes the left lower jaw, without postdentary bones, bearing the root of the last incisor, canine and four postcanines plus one partial crown inside the dentary, not erupted, and two maxillary fragments, one with a broken canine and another with one postcanine. The features of the lower jaw and lower/upper postcanines resemble those of the prozostrodontians Prozostrodon brasiliensis from the older Hyperodapedon AZ and Brasilodon quadrangularis and Brasilitherium riograndensis from the same Riograndia AZ. The inclusion of Botucaraitherium within a broad phylogenetic analysis, positioned it as a more derived taxon than tritylodontids, being the sister-taxon of Brasilodon, Brasilitherium plus Mammaliaformes. Although the new taxon is based on few cranial elements, it represents a additional faunal component of the Triassic Riograndia AZ of southern Brazil, in which small-sized derived non-mammaliaform cynodonts, closely related to the origin of mammaliaforms, were ecologically well succeed and taxonomically diverse.

  14. Changes in television viewing and computers/videogames use among high school students in Southern Brazil between 2001 and 2011.

    PubMed

    Silva, Kelly Samara; da Silva Lopes, Adair; Dumith, Samuel Carvalho; Garcia, Leandro Martin Totaro; Bezerra, Jorge; Nahas, Markus Vinicius

    2014-02-01

    To compare the prevalence of television (TV) watching and of computer/videogame use among high school students (15-19 years) from Southern Brazil between 2001 and 2011 and to identify associated socio-demographic factors. Panel studies were conducted with high school students in the state of Santa Catarina, Brazil, in 2001 (n = 5,028) and 2011 (n = 6,529). TV watching and computer/videogame use were collected using questionnaires. Prevalence of ≥2 h/day of TV watching dropped from 76.8 to 61.5 % and ≥2 h/day of computer/videogame use increased from 37.9 to 60.6 %. In both surveys, those aged 15-16 and those who did not work had higher likelihoods of being exposed to ≥2 h/day of TV watching. Boys, those with higher family income, and those who were living in urban areas had higher likelihoods of ≥2 h/day of computer/videogame use. Older age, studying at night and not working were protective factors to these behaviors. After a decade, there was a decrease in the prevalence of TV viewing and an increase in computer/videogame use. Socio-demographic factors were differently associated with these behaviors.

  15. Genetic and phylogenetic analyses of capsid protein gene in feline calicivirus isolates from Rio Grande do Sul in southern Brazil.

    PubMed

    Henzel, A; Sá e Silva, M; Luo, S; Lovato, L T; Weiblen, R

    2012-02-01

    Feline calicivirus (FCV) is an important pathogen that affects domestic cats, inducing acute oral and upper respiratory tract clinical signs. The aim of this study was to analyze the variability of the capsid protein in different FCV isolates from southern Brazil. The sequencing analyses of thirteen Brazilian FCV samples, phylogenetic analyses and assessments of ten previously published sequences were conducted by examining the open reading frame 2 (ORF2, regions B-F). Comparisons of the predicted amino acid sequences of the ORF2 in Brazilian FCV isolates with those of the FCV-F9 strain indicated that the main differences are located within the regions C and hypervariable E (HVR_E). Epitopes that were mapped to the regions D, 5'HVR_E and conserved E also presented with some variability when compared to the strain F9. This is the first study describing sequence analyses and the phylogenetic relationships among FCV isolates from Brazil. The results presented here may expand upon current knowledge regarding aspects of FCV biology, epidemiology and genetic diversity and provide insights into improving the efficacies of current FCV vaccines. Copyright © 2011 Elsevier B.V. All rights reserved.

  16. Cytisus scoparius (Fam. Fabaceae) in southern Brazil - first step of an invasion process?

    PubMed

    Cordero, Rodrigo León; Torchelsen, Fábio P; Overbeck, Gerhard E; Anand, Madhur

    2016-03-01

    The occurrence of Scotch broom Cytisus scoparius (L.) Link (Fabaceae), is reported for the first time in Brazil. The species has been registered in the species-rich Campos Sulinos grasslands, in the Campos de Cima da Serra, and in the Serra do Sudeste. Naturalizing populations were frequently formed in natural habitats near to human settlements, where prevailing land uses and disturbances facilitate dispersal and establishment. The plant is an invasive species that has globally caused significant damage to biodiversity and economic losses. In Brazil, the species has a strong potential for spreading into a wide range of ecosystems. The Atlantic Forest biome and part of the Pampa biome, together known as the Campos Sulinos, represent optimal areas for the species. Features of the observed populations and recommendations for management are presented.

  17. Pathologies of Oligacanthorhynchus pardalis (Acanthocephala, Oligacanthorhynchidae) in Leopardus tigrinus (Carnivora, Felidae) in Southern Brazil.

    PubMed

    Gallas, Moisés; da Silveira, Eliane Fraga; da Silvera, Eliane Fraga

    2012-01-01

    In Brazil, Oligacanthorhynchus pardalis (Westrumb, 1821) Schmidt, 1972 has been observed in five species of wild felines. In the present study, five roadkilled oncillas (Leopardus tigrinus Schreber, 1775) were collected in the State of Rio Grande do Sul, Brazil. Chronic lesions caused by O. pardalis were observed in the small intestine of one of the specimens. Histological examination identified a well-defined leukocyte infiltration and an area of collagenous fibrosis. Only males parasites (n = 5) were found, with a prevalence of 20%. The life cycle of Oligacanthorhynchus species is poorly known, although arthropods may be their intermediate hosts. The low prevalence encountered may be related to the small number of hosts examined, and the reduced ingestion of arthropods infected by larvae of O. pardalis. This is the first report of O. pardalis parasitizing L. tigrinus in the Brazilian state of Rio Grande do Sul.

  18. Impact of the El Niño/Southern Oscillation on visceral leishmaniasis, Brazil.

    PubMed

    Franke, Carlos Roberto; Ziller, Mario; Staubach, Christoph; Latif, Mojib

    2002-09-01

    We used time-series analysis and linear regression to investigate the relationship between the annual Niño-3 index from 1980 to 1998 and the annual incidence of visceral leishmaniasis (VL) in the State of Bahia, Brazil, during 1985-1999. An increase in VL incidence was observed in the post-El Niño years 1989 (+38.7%) and 1995 (+33.5%). The regression model demonstrates that the previous year's mean Niño-3 index and the temporal trend account for approximately 50% of the variance in the annual incidence of VL in Bahia. The model shows a robust agreement with the real data, as only the influence of El Niño on the cycle of VL was analyzed. The results suggest that this relationship could be used to predict high-risk years for VL and thus help reduce health impact in susceptible regions in Brazil.

  19. Population structure, sex ratio and growth of the seabob shrimp Xiphopenaeus kroyeri (Decapoda, Penaeidae) from coastal waters of southern Brazil

    PubMed Central

    Grabowski, Raphael Cezar; Simões, Sabrina Morilhas; Castilho, Antonio Leão

    2014-01-01

    Abstract This study evaluated the growth and population structure of Xiphopenaeus kroyeri in Babitonga Bay, southern Brazil. Monthly trawls were conducted from July 2010 through June 2011, using a shrimp boat outfitted with double-rig nets, at depths from 5 to 17 m. Differences from the expected 0.5 sex ratio were determined by applying a Binomial test. A von Bertalanffy growth model was used to estimate the individual growth, and longevity was calculated using its inverted formula. A total of 4,007 individuals were measured, including 1,106 juveniles (sexually immature) and 2,901 adults. Females predominated in the larger size classes. Males and females showed asymptotic lengths of 27.7 mm and 31.4 mm, growth constants of 0.0086 and 0.0070 per day, and longevities of 538 and 661 days, respectively. The predominance of females in larger size classes is the general rule in species of Penaeidae. The paradigm of latitudinal-effect does not appear to apply to seabob shrimp on the southern Brazilian coast, perhaps because of the small proportion of larger individuals, the occurrence of cryptic species, or the intense fishing pressure in this region. The longevity values are within the general range for species of Penaeidae. The higher estimates for longevity in populations at lower latitudes may have occurred because of the growth constants observed at these locations, resulting in overestimation of this parameter. PMID:25561841

  20. Population structure, sex ratio and growth of the seabob shrimp Xiphopenaeuskroyeri (Decapoda, Penaeidae) from coastal waters of southern Brazil.

    PubMed

    Grabowski, Raphael Cezar; Simões, Sabrina Morilhas; Castilho, Antonio Leão

    2014-01-01

    This study evaluated the growth and population structure of Xiphopenaeuskroyeri in Babitonga Bay, southern Brazil. Monthly trawls were conducted from July 2010 through June 2011, using a shrimp boat outfitted with double-rig nets, at depths from 5 to 17 m. Differences from the expected 0.5 sex ratio were determined by applying a Binomial test. A von Bertalanffy growth model was used to estimate the individual growth, and longevity was calculated using its inverted formula. A total of 4,007 individuals were measured, including 1,106 juveniles (sexually immature) and 2,901 adults. Females predominated in the larger size classes. Males and females showed asymptotic lengths of 27.7 mm and 31.4 mm, growth constants of 0.0086 and 0.0070 per day, and longevities of 538 and 661 days, respectively. The predominance of females in larger size classes is the general rule in species of Penaeidae. The paradigm of latitudinal-effect does not appear to apply to seabob shrimp on the southern Brazilian coast, perhaps because of the small proportion of larger individuals, the occurrence of cryptic species, or the intense fishing pressure in this region. The longevity values are within the general range for species of Penaeidae. The higher estimates for longevity in populations at lower latitudes may have occurred because of the growth constants observed at these locations, resulting in overestimation of this parameter.

  1. Diet of the lizard Liolaemus occipitalis in the coastal sand dunes of southern Brazil (Squamata-Liolaemidae).

    PubMed

    Verrastro, L; Ely, I

    2015-05-01

    Knowledge of a species' diet provides important information on adaptation and the relationship between the organism and its environment. The genus Liolaemus occurs in the southern region of South America and is an excellent model to investigate the adaptive processes of vertebrate ecology in ecosystems of this region of the world. Liolaemus occipitalis is an endangered species that inhabits the coastal sand dunes of southern Brazil. This species is the most abundant vertebrate in this environment, and it presents unique adaptation characteristics to the restinga environment. The present study analyzed this lizard's diet to verify similarities or differences between this species and other species of the same genus. Specimens were collected monthly from January 1996 to December 1997. The number of items, frequency of occurrence and volume of each prey taxon were determined. Arthropods were identified to the order level, and plant material was identified as flower, fruit, seed and leaves. Variations in the diet of males and females, adults and juveniles and seasons were also analyzed. The data indicate that Liolaemus occipitalis is a generalist, "sit-and-wait" or ambush predator as well as omnivorous, feeding on both arthropods and plant material. Significant ontogenetic differences were verified. Juveniles are more carnivorous, and the intake of plant material increases with size and age. Seasonal differences in diet composition were also observed. In the spring, arthropod and plant materials were more diversified and, therefore, consumed more often.

  2. Evaluation of different methods to estimate daily reference evapotranspiration in ungauged basins in Southern Brazil

    NASA Astrophysics Data System (ADS)

    Ribeiro Fontoura, Jessica; Allasia, Daniel; Herbstrith Froemming, Gabriel; Freitas Ferreira, Pedro; Tassi, Rutineia

    2016-04-01

    Evapotranspiration is a key process of hydrological cycle and a sole term that links land surface water balance and land surface energy balance. Due to the higher information requirements of the Penman-Monteith method and the existing data uncertainty, simplified empirical methods for calculating potential and actual evapotranspiration are widely used in hydrological models. This is especially important in Brazil, where the monitoring of meteorological data is precarious. In this study were compared different methods for estimating evapotranspiration for Rio Grande do Sul, the Southernmost State of Brazil, aiming to suggest alternatives to the recommended method (Penman-Monteith-FAO 56) for estimate daily reference evapotranspiration (ETo) when meteorological data is missing or not available. The input dataset included daily and hourly-observed data from conventional and automatic weather stations respectively maintained by the National Weather Institute of Brazil (INMET) from the period of 1 January 2007 to 31 January 2010. Dataset included maximum temperature (Tmax, °C), minimum temperature (Tmin, °C), mean relative humidity (%), wind speed at 2 m height (u2, m s-1), daily solar radiation (Rs, MJ m- 2) and atmospheric pressure (kPa) that were grouped at daily time-step. Was tested the Food and Agriculture Organization of the United Nations (FAO) Penman-Monteith method (PM) at its full form, against PM assuming missing several variables not normally available in Brazil in order to calculate daily reference ETo. Missing variables were estimated as suggested in FAO56 publication or from climatological means. Furthermore, PM was also compared against the following simplified empirical methods: Hargreaves-Samani, Priestley-Taylor, Mccloud, McGuiness-Bordne, Romanenko, Radiation-Temperature, Tanner-Pelton. The statistical analysis indicates that even if just Tmin and Tmax are available, it is better to use PM estimating missing variables from syntetic data than

  3. The taxonomy of some Poecilacanthoidea (Eucestoda: Trypanorhyncha) from elasmobranchs off the southern coast of Brazil.

    PubMed

    Pinto, R M; Knoff, M; São Clemente, S C; Lanfredi, R M; Gomes, D C

    2006-09-01

    Specimens of elasmobranchs, collected in the State of Santa Catarina, Brazil, were parasitized with Poecilacanthoidea trypanorhynch cestodes. Sharks of the species Prionace glauca harboured Callitetrarhynchus gracilis and Floriceps saccatus, and those of the species Sphyrna zygaena were infected with Callitetrarhynchus speciosus. Details of the proglottids of F. saccatus, provided by bright-field, and/or scanning electron microscopy, are described. Adults of F. saccatus are reported for the first time in the Brazilian coast.

  4. Occurrence of 15 Haplotypes of Linepithema micans (Hymenoptera: Formicidae) in Southern Brazil.

    PubMed

    Ramalho, Manuela Oliveira; Martins, C; Campos, T; Nondillo, A; Botton, M; Bueno, O C

    2017-08-01

    The ant genus Linepithema is widely known, thanks to the pest species Linepithema humile (Mayr), which is easily mistaken for Linepithema micans (Forel) due to their morphological similarity. Like L. humile, L. micans is associated to the main grapevine pest in Brazil, Eurhizococcus brasiliensis (Wille), also known as ground pearl. Therefore, the present study uses mtDNA fragments to expand the knowledge of haplotype diversity and distribution of L. micans in the state of Rio Grande do Sul (Brazil), to understand the genetic differences of the populations identified in this study. We identified 15 haplotypes of L. micans spread across different localities. Twelve of these haplotypes were new for the species. The high haplotype diversity uncovered in Rio Grande do Sul (Brazil) for this species was predictable, as L. micans is in its native environment. Additional studies that take gene flow into account may reveal interesting aspects of diversity in these populations. © The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  5. Coastline orientation, aeolian sediment transport and foredune and dunefield dynamics of Moçambique Beach, Southern Brazil

    NASA Astrophysics Data System (ADS)

    Miot da Silva, Graziela; Hesp, Patrick

    2010-08-01

    This paper examines the role of coastline orientation on foredune and dunefield development at Moçambique Beach, Southern Brazil. The beach displays significant alongshore variations in exposure to the prevailing winds and waves, grain size, beach/surfzone morphodynamic type, foredune volume, and type and dimensions of the Holocene dune systems. Two wind analyses and calculation of aeolian drift potentials were carried out, one based on 1 year of wind record and another based on a record of 34 years. Monthly topographic surveys of beach and foredunes on 5 profiles along Moçambique beach were conducted over one year to obtain data on beach mobility and width, aeolian sediment transport and foredune development. Southerly winds dominate, and aeolian sediment supply is minimal in the south, moderate in the central portion and high in the northern portion of the embayment. The relationship between actual sediment supply, foredune building and potential sediment supply is relatively poor over one year due to factors such as beach type and mobility, beach moisture levels, rainfall, storm surge and wave scarping. The intermediate term (34 year) record indicates a strong relationship between foredune size and volume, winds and shoreline orientation: foredune volume is minimum in the southern part of the beach and greatest in the northern part of the beach. The Holocene barrier and dunefield development also reflects the long term control of shoreline orientation and increasing longshore gradient in exposure to southerly winds: for the last 6000-7000 years a small foredune developed in the southern portion, parabolics and small transgressive dunes in the central portion, and a large-scale transgressive dunefield in the northern portion.

  6. Detection of Alphacoronavirus in velvety free-tailed bats (Molossus molossus) and Brazilian free-tailed bats (Tadarida brasiliensis) from urban area of Southern Brazil.

    PubMed

    Lima, Francisco Esmaile de Sales; Campos, Fabrício Souza; Kunert Filho, Hiran Castagnino; Batista, Helena Beatriz de Carvalho Ruthner; Carnielli, Pedro; Cibulski, Samuel Paulo; Spilki, Fernando Rosado; Roehe, Paulo Michel; Franco, Ana Cláudia

    2013-08-01

    A survey was carried out in search for bat coronaviruses in an urban maternity roost of about 500 specimens of two species of insectivorous bats, Molossus molossus and Tadarida brasiliensis, in Southern Brazil. Twenty-nine out of 150 pooled fecal samples tested positive by reverse transcription-PCR contained fragments of the RNA-dependent RNA polymerase gene of coronavirus-related viruses. The sequences clustered along with bat alphacoronaviruses, forming a subcluster within this group. Our findings point to the need for risk assessment and continued surveillance of coronavirus infections of bats in Brazil.

  7. Conserving the grassland Important Bird Areas (IBAs) of southern South America: Argentina, Uruguay, Paraguay, and Brazil

    Treesearch

    Adrian S. Di Giacomo; Santiago Krapovickas

    2005-01-01

    In the southern part of South America, knowledge about bird species distribution is still not used as a tool for land use planning and conservation priority-setting. BirdLife International’s Important Bird Areas (IBA) Program is an appropriate vehicle for analyzing existing information about birds, and to generate new data where necessary. IBA inventories...

  8. Molecular characterization of host strains of Spodoptera frugiperda(Lepidoptera:Noctuidae)in southern Brazil :

    USDA-ARS?s Scientific Manuscript database

    Fall armyworm infestations in most of North America north of Mexico arise from annual migrations of populations that overwinter in southern Texas and Florida. Mapping the pattern of migration and the relative contributions of the Texas and Florida populations would contribute both to our understandi...

  9. Batkoa apiculata (Thaxter) Humber affecting Anopheles (Diptera: Culicidae) in the municipality of Una, Southern Bahia, Brazil

    USDA-ARS?s Scientific Manuscript database

    Surveys for fungal pathogens affecting adult mosquitoes from the genus Anopheles were conducted in flooded and swamp-like natural breeding sites near residences in the center and suburbs of the city of Una as well as the nearby village of Outeiro in southern Bahia. Surveys of 54 mosquito breeding si...

  10. Geochemical and ecotoxicological assessment for estuarine surface sediments from Southern Brazil.

    PubMed

    Rodrigues, Sarah K; Abessa, Denis M S; Machado, Eunice C

    2013-10-01

    Sediments from Guaratuba Bay (PR, Brazil), a marine protected area, were collected and evaluated for geochemistry and toxicity. High levels of P and acute toxicity were observed in some samples. Concentrations of Cu, Cd, Pb and Zn were relatively low; however, Cd levels eventually exceeded Threshold Effect Level. Toxicities were associated to nutrients and metals enrichment. Results suggest that impacts are incipient and occur only at specific sites, associated to multiple contamination sources. Despite sediments quality seems to range between good and fair, attention is required to land-use planning around Guaratuba Bay and controlling local pollution sources. Copyright © 2013 Elsevier Ltd. All rights reserved.

  11. A new species of Paracaprella Mayer, 1890 (Amphipoda: Caprellida: Caprellidae) from southern Brazil.

    PubMed

    Lacerda, Mariana B; Masunari, Setuko

    2014-12-23

    A new species of the genus Paracaprella is described based on the specimens associated with the algae Sargassum cymosum and Laurencia obtusa that were collected from infralittoral depths (0.5 to 3.0 m) at Sepultura Beach, Bombinhas and Paciência Beach, Penha, Santa Catarina State, Brazil. This new species differs from the others of the genus by the unique morphology of the males' gnathopod 2: its propodus has a grasping margin provided with a deep groove. An identification key for Paracaprella species is also presented.

  12. Rhyacian A-type tholeiitic granites in southern Brazil: Geochemistry, U-Pb zircon ages and Nd model ages

    NASA Astrophysics Data System (ADS)

    Mesquita, Maria José; Bitencourt, Maria de Fátima; Nardi, Lauro Stoll; Picanço, Jefferson; Chemale, Farid, Jr.; Pimenta, Vanessa de Almeida

    2017-04-01

    In the southern South American platform, 2.5 to 2.0 Ga terranes, probably related to the Atlantica supercontinent, occur mainly as minor reworked inliers within Neoproterozoic, Brasiliano/Pan-African orogenic belts, as the Ribeira Belt in southern Brazil. The dispersion of such fragments has generated uncertainties about their geotectonic reconstruction, and their study has been supported mainly by elemental and isotope geochemistry. The southern Ribeira Belt lies between the Paranapanema and Luiz Alves cratons and contains reworked Neoarquean and Paleoproterozoic terranes which outcrop as basement nuclei in supracrustal sequences, as the Setuva Complex. The Água Comprida Suite, situated in the northern part of the Setuva Complex, consists of Amphibole-Biotite Syenogranite (ABS), Porphyritic Biotite Syenogranite (PBS), and Equigranular Biotite Syenogranite (EBS). All granites are foliated and intensively deformed. The oldest foliation (Sn) is marked by augen feldspars set in a recrystallized matrix, followed by a crenulation cleavage (Sn+1) which evolves to discrete shear zones. ABS is a metaluminous, reduced A-type granite with FeOt / (FeOt + MgO) > 0.9, with high HFSE and REE contents, corresponding to magmas related to continental medium to high-K tholeiitic series. PBS and specially EBS are highly differentiated, metaluminous to peraluminous (EBS), oxidized granites. The increase of Al2O3 and Rb, and decrease of HFS and RE elements relative to ABS indicate their evolution from tholeiitic magmas. The Água Comprida Suite granites are cogenetic rocks evolved from a within-plate mantle source, marked by high Nb, Ta, and Y. The influence of previously metasomatised mantle sources is evidenced by negative Nb, Ti, and P anomalies. The age of ABS is 2187 ± 26 Ma, and that of PBS is between 2180 ± 13 to 2186 ± 22 Ma. The Nd model age of 2.4 Ga, and ɛNd(2.18 Ga) between - 0.23 and - 0.27 support the interpretation of ABS being formed from juvenile material with a

  13. Food habits and nutritional status of agricultural migrant workers in Southern Brazil.

    PubMed

    Desai, I D; Garcia Tavares, M L; Dutra de Oliveira, B S; Douglas, A; Duarte, F A; Dutra de Oliveira, J E

    1980-03-01

    A new class of migrant workers, commonly known as "Boia-Frias", is rapidly growing in the periurban slumbs (favelas) of Brazil. In 1978 a collaborative study was undertaken to assess the food habits and nutritional status of 100 migrant worker families of Vila Recreio, a typical Boia-Fria settlement near Ribeirao Preto in the state of Sao Paulo. The findings of this survey revealed that the traditional diet of Boia-Frias is nutritionally inadequate both in quality and quantity. Their rice and bean-based diet lacks sufficient variety because of the infrequent use of fresh fruits and vegetables, which are available locally, and of supplemental amounts of protein-rich foods of animal origin. Empty-calorie foods such as carbonated drinks and alcoholic beverages are consumed freely; and starchy foods, traditionally used in the North and Northeast of Brazil, are used commonly as weaning foods. Although dietary practices of pregnant and lactating women are poor, breast-feeding is still practiced by most mothers. The biochemical analysis of blood samples did not indicate major subclinical deficiencies except low hematological values and low plasma vitamin A concentrations in about 25% of the population examined. Plasma cholesterol and plasma vitamin E values were found to be normal. However, anthropometric examinations revealed clear signs of malnutrition and/or undernourishment, which likely impairs their capacity for physical work and adversely affects their overall health.

  14. Spatiotemporal Distribution and Population Structure of Monokalliapseudes schubarti (Tanaidacea: Kalliapseudidae) in an Estuary in Southern Brazil

    PubMed Central

    Freitas-Júnior, Felipe; Christoffersen, Martin Lindsey; de Araújo, Joafrâncio Pereira; Branco, Joaquim Olinto

    2013-01-01

    Monokalliapseudes schubarti is an endemic tanaidacean microcrustacean from southeastern Brazil to Uruguay inhabiting low energy estuaries. Saco da Fazenda is located in the estuary of the Itajaí-Açú River, state of Santa Catarina, Brazil. It is exposed to strong anthropic impact and receives intensive flows of domestic wastewater, solid residues, and drainage activities. Specimens of M. schubarti were collected monthly, in the intertidal and subtidal regions of Saco da Fazenda, in four stations defined as a function of the physiography of the environment during the period of July 2003 to June 2004. Fecundity values were high, with continuous reproductive activity during the whole period of study. The greatest population densities were observed in the intertidal region, where they are nevertheless intensely consumed by birds, swimming crabs, and fish. This species represents a fundamental link in the food chain of Saco da Fazenda, transferring energy from the detritus level to higher trophic levels. Habitat disturbance and high organic matter may represent factors controlling the distribution of populations of M. schubarti. For this reason, the species may be used to monitor anthropic effects in estuarine areas. PMID:24298211

  15. [Cost-effectiveness of an organized breast cancer screening program in Southern Brazil].

    PubMed

    Ribeiro, Rodrigo Antonini; Caleffi, Maira; Polanczyk, Carisi Anne

    2013-11-01

    The aim of this study was to evaluate the cost-effectiveness of an organized breast cancer mammographic screening program implemented in Porto Alegre (Núcleo Mama Porto Alegre - NMPOA), Rio Grande do Sul State, Brazil. A Markov model was constructed to estimate the incremental cost-effectiveness ratio of NMPOA compared to current BC diagnosis and care in the Brazilian public health system, in a hypothetical cohort of women aged 40-69 years at risk of developing breast cancer. Model parameters were collected from NMPOA and the national literature. In the NMPOA strategy, effectiveness was modeled taking into account the actual observed screening adherence. Effectiveness was measured in quality-adjusted life years (QALYs). Incremental cost-effectiveness ratio in the base case was R$ 13,426 per QALY. This result was not sensitive to variation in the main model parameters in sensitivity analyses. Considering the threshold usually suggested as highly attractive in Brazil, breast cancer screening as performed in NMPOA is cost-effective in cities with high incidence of breast cancer.

  16. Prevalence of self-reported halitosis and associated factors in adolescents from Southern Brazil.

    PubMed

    Lopes, Montalverne Hb; Rösing, Cassiano K; Colussi, Paulo Roberto G; Muniz, Francisco Wilker Mg; Linden, Maria Ss

    2016-09-01

    The aim of this study was to assess the prevalence of selfreported halitosis and associated factors in adolescents from Passo Fundo, Brazil. Additionally, we studied adolescents' concern with their own breath and whether anyone had ever told them that they had halitosis. A crosssectional observational survey was conducted on 15to 19year old high school students from public and private schools in the city of Passo Fundo, Brazil. The random sample consisted of 736 adolescents from 20 schools. An interview with a structured questionnaire was administered. The prevalence of selfreported halitosis and associated factors was analyzed by either the chisquare test or Fisher's exact test. The prevalence of selfreported halitosis was 39.67%. The mother's level of education was associated with the outcome. Gender and behavioral habits were not associated with selfreported halitosis. Halitosis reported by others presented a prevalence of 10.73% and was associated to male gender and oral hygiene habits. Individuals with less toothbrushing frequency and no use of dental floss were associated to halitosis reported by others. It was observed that 88.58% of the participants are concerned with halitosis. It is concluded that there is high prevalence of selfreported halitosis, which is associated with a socioeconomic pattern. Most adolescents report concern with their own breath.

  17. SIXTEEN YEARS OF PITYRIASIS VERSICOLOR IN METROPOLITAN AREA OF PORTO ALEGRE, SOUTHERN BRAZIL

    PubMed Central

    HEIDRICH, Daiane; DABOIT, Tatiane Caroline; STOPIGLIA, Cheila Denise Ottonelli; MAGAGNIN, Cibele Massotti; VETORATTO, Gerson; AMARO, Taís Guarienti; SCROFERNEKER, Maria Lúcia

    2015-01-01

    SUMMARY Pityriasis versicolor is the most common of the diseases caused by Malasseziayeasts. The aim of this study is to determine the prevalence of pityriasis versicolor and its etiological aspects in the metropolitan area of Porto Alegre, Brazil. A retrospective crosssectional study with data from patients of a reference hospital from 1996 to 2011 was performed. Collected data included: date, age, gender, ethnicity, anatomical region of lesion and the direct mycological examination results. Among the positive results in the direct mycological examination, 5.8% (2,239) were positive for pityriasis versicolor. The angular coefficient (B) was -0.3%/year, showing a decrease over the years. The disease was more prevalent in men (7.1% of men versus 5.1% of women that underwent the direct mycological examination); younger age (median 31 years old); "pardo" and black people (3.7% more than expected in the sample); trunk (73.44% of the affected anatomic sites). Lesions in rare sites (groin, genitals, legs, feet and hands) were also observed in this study. In conclusion, due to the decrease in the prevalence of pityriasis versicolor, long-term epidemiological studies in the metropolitan area of Porto Alegre, Brazil, are needed to continue the monitoring of this disease. PMID:26422149

  18. Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil.

    PubMed

    Prim, Rodrigo Ivan; Schörner, Marcos André; Senna, Simone Gonçalves; Nogueira, Christiane Lourenço; Figueiredo, Anna Carolina Cançado; Oliveira, Jaquelline Germano de; Rovaris, Darcita Bürger; Bazzo, Maria Luiza

    2015-08-01

    Drug resistance is a global threat and one of the main contributing factors to tuberculosis (TB) outbreaks. The goal of this study was to analyse the molecular profile of multidrug-resistant TB (MDR-TB) in the state of Santa Catarina in southern Brazil. Fifty-three MDR Mycobacterium tuberculosis clinical isolates were analysed by spoligotyping and a partial region of the rpoB gene, which is associated with rifampicin resistance (RMP-R), was sequenced. Some isolates were also distinguished by their mycobacterial interspersed repetitive units (MIRU). S531L was the most prevalent mutation found within rpoB in RMP-R isolates (58.5%), followed by S531W (20.8%). Only two MDR isolates showed no mutations within rpoB. Isolates of the Latin American Mediterranean (LAM) family were the most prevalent (45.3%) found by spoligotyping, followed by Haarlem (9.4%) and T (7.5%) families. SIT106 was found in 26.4% of isolates and all SIT106 isolates typed by MIRU-12 (5 out of 14) belong to MIT251. There was a high correlation between the S531W mutation and the LAM family mainly because all SIT2263 (LAM9) isolates carry this mutation. Among isolates with the S531W mutation in rpoB MIRU demonstrates a cluster formed by four isolates (SIT2263 and MIT163) and very similar profiles were observed between eight of the nine isolates. Better characterisation of TB isolates may lead to new ways in which to control and treat TB in this region of Brazil.

  19. Genetic characterization of hantaviruses associated with sigmodontine rodents in an endemic area for hantavirus pulmonary syndrome in southern Brazil.

    PubMed

    de Oliveira, Renata Carvalho; Padula, Paula J; Gomes, Raphael; Martinez, Valeria P; Bellomo, Carla; Bonvicino, Cibele R; Freire e Lima, Danúbia Inês; Bragagnolo, Camila; Caldas, Antônio C S; D'Andrea, Paulo S; de Lemos, Elba R S

    2011-03-01

    An ecological assessment of reservoir species was conducted in a rural area (Jaborá) in the mid-west of the state of Santa Catarina in southern Brazil, where hantavirus pulmonary syndrome is endemic, to evaluate the prevalence of hantavirus infection in wild rodents. Blood and tissue samples were collected from 507 rodents during seven field trips from March 2004 to April 2006. Some of the animals were karyotyped to confirm morphological identification. Phylogenetic reconstructions of rodent specimens, based on the mitochondrial DNA cytochrome b gene sequences, were also obtained. Hantavirus antibody was found in 22 (4.3%) of the 507 rodents: 5 Akodon montensis, 2 Akodon paranaensis, 14 Oligoryzomys nigripes, and 1 Sooretamys angouya. Viral RNAs detected in O. nigripes and A. montensis were amplified and sequenced. O. nigripes virus genome was 97.5% (nt) and 98.4% (nt) identical to sequences published for Araucaria (Juquitiba-like) virus based on N and G2 fragment sequences. Viral sequences from A. montensis strain showed 89% and 88% nucleotide identities in a 905-nt fragment of the nucleocapsid (N) protein-coding region of the S segment when it was compared with two other Akodontine rodent-associated viruses from Paraguay, A. montensis and Akodon cursor, respectively. Phylogenetic analysis showed the cocirculation of two genetic hantavirus lineages in the state of Santa Catarina, one from O. nigripes and the other from A. montensis, previously characterized in Brazil and Paraguay, respectively. The hantavirus associated with A. montensis, designed Jaborá virus, represents a distinct phylogenetic lineage among the Brazilian hantaviruses.

  20. Diel and seasonal movements of grumatã Prochilodus lineatus (Valenciennes 1836) (Characiformes: Prochilodontidae) in the Sinos River, Southern Brazil.

    PubMed

    Pesoa, N A; Schulz, U H

    2010-12-01

    Prochilodus lineatus is a reophilic migratory species of economical importance for local fisheries which is widely distributed in Brazil. The present study investigated diel and seasonal movement patterns, spawning migration and habitat use of P. lineatus in the Sinos River, Southern Brazil. Between August 2002 and March 2004, 19 grumatãs were tagged internally with digitally coded radio transmitters. Tracking was conducted weekly by boat or aircraft, and six fixed data loggers recorded movements of tagged fish. Results showed that the mean distance covered per fish and day was positively related with fish weight (p = 0.03; n = 19). Grumatã showed preference for river streches surrounded by wetlands (p < 0.001; n = 286). Their diel activity pattern displayed one peak at 7 hours, and a second at 15 hours. During the night, activity was significantly reduced (p = 0.01). The seasonal movements pattern showed increased activity in October 2002 - March 2003 and from October 2003 to January 2004. Multiple regression analysis did not show a significant relationship between distance covered and temperature or water level (p = 0.116; n = 19). No synchronized long distance spawning migrations were observed. The mean distance covered by the individuals/day showed a negative significant relationship with number of tracking days (p = 0.022; n = 19), indicating a higher probability of being captured for more mobile individuals. The preservation of the still existing wetlands and reduction of the fishing pressure are essential for the future conservation of the grumatã population of the Sinos River.

  1. Epidemiology of Rickettsia sp. strain Atlantic rainforest in a spotted fever-endemic area of southern Brazil.

    PubMed

    Barbieri, Amalia R M; Filho, Jonas M; Nieri-Bastos, Fernanda A; Souza, Julio C; Szabó, Matias P J; Labruna, Marcelo B

    2014-10-01

    The present study was performed in Vila Itoupava, an area of the state of Santa Catarina, southern Brazil, in which a tick-borne spotted fever illness has been endemic since 2003. Notably, both the etiological agent and the vector of these spotted fever cases remain unknown. During January 2011, humans, domestic dogs, and their ticks were sampled in households that are typically surrounded by highly preserved Atlantic rainforest fragments. Ticks collected from dogs were Amblyomma ovale (34% prevalence), Amblyomma aureolatum (18.9%), and Rhipicephalus sanguineus (3.8%). A total of 7.8% (6/77) A. ovale and 9.3% (4/43) A. aureolatum were infected by Rickettsia sp. strain Atlantic rainforest, a Rickettsia parkeri-like agent recently shown to cause spotted fever illness in southeastern Brazil. Overall, 67.3% (35/52) of the dogs were seroreactive to spotted fever group rickettsiae, mostly with highest endpoint titers to R. parkeri. Among humans, 46.7% (7/15) reacted serologically to rickettsiae at low to moderate endpoint titers. Because canine seroreactivity to R. parkeri was strongly associated with frequent contact with forests (the preferred habitat for A. ovale and A. aureolatum), it is concluded that sampled dogs have been infected by strain Atlantic rainforest through the parasitism of these tick species. The present study provides epidemiological evidence that the spotted fever in the study area has been caused by Rickettsia sp. strain Atlantic rainforest, transmitted to humans by either A. ovale or A. aureolatum. Further studies encompassing direct diagnostic methods on clinical specimens from patients are needed to confirm the above epidemiological evidence. Copyright © 2014 Elsevier GmbH. All rights reserved.

  2. The prevalence of trichomoniasis and associated factors among women treated at a university hospital in southern Brazil

    PubMed Central

    2017-01-01

    Background Trichomoniasis is the most prevalent non-viral sexually transmitted disease (STD) in the world; however, it remains a neglected parasitic disease. This study aimed to determine the prevalence of trichomoniasis and its associated epidemiological factors among women treated at a hospital in southern Brazil. Methodology/Principal findings A cross-sectional study was performed to determine the prevalence of this infection in women treated at Hospital Universitário (HU) in Rio Grande, Rio Grande do Sul, Brazil, between January 2012 and January 2015. This study consisted a self-administered questionnaire regarding demographic, clinical, and behavioural data and a molecular diagnosis with polymerase chain reaction (PCR) using the TVK3/7 primer set, which was confirmed with sequence analysis. Of the 345 women surveyed, the overall prevalence of Trichomonas vaginalis (T. vaginalis) was 4.1% (14/345). The prevalence rates were 5.9% among pregnant women, 8.5% among HIV-positive women, and 10.1% among HIV-positive pregnant women. The rates for groups with other significant demographic and clinical features were as follows: 6.6% among women with white skin, 12.3% among women with an income below the minimum monthly wage, 7.4% among women with a vaginal pH greater than or equal to 4.6, and 7.9% among women with a comorbid STD. The multivariate analysis confirmed that pregnant women who were HIV-positive (p = 0.001) and had low incomes (p = 0.026) were the most likely to have this infection. Conclusions A multivariate analysis confirmed that HIV-positive pregnant women with low incomes were the participants most likely to have trichomoniasis. These results are important because this Brazilian region presents a high prevalence of HIV-1 subtype C, which is associated with greater transmissibility. Additionally, low family income reveals a socioeconomic fragility that might favour the transmission of this STD. PMID:28346531

  3. Assessment of inhaler techniques employed by patients with respiratory diseases in southern Brazil: a population-based study*

    PubMed Central

    de Oliveira, Paula Duarte; Menezes, Ana Maria Baptista; Bertoldi, Andréa Dâmaso; Wehrmeister, Fernando César; Macedo, Silvia Elaine Cardozo

    2014-01-01

    OBJECTIVE: To identify incorrect inhaler techniques employed by patients with respiratory diseases in southern Brazil and to profile the individuals who make such errors. METHODS: This was a population-based, cross-sectional study involving subjects ≥ 10 years of age using metered dose inhalers (MDIs) or dry powder inhalers (DPIs) in 1,722 households in the city of Pelotas, Brazil. RESULTS: We included 110 subjects, who collectively used 94 MDIs and 49 DPIs. The most common errors in the use of MDIs and DPIs were not exhaling prior to inhalation (66% and 47%, respectively), not performing a breath-hold after inhalation (29% and 25%), and not shaking the MDI prior to use (21%). Individuals ≥ 60 years of age more often made such errors. Among the demonstrations of the use of MDIs and DPIs, at least one error was made in 72% and 51%, respectively. Overall, there were errors made in all steps in 11% of the demonstrations, whereas there were no errors made in 13%.Among the individuals who made at least one error, the proportion of those with a low level of education was significantly greater than was that of those with a higher level of education, for MDIs (85% vs. 60%; p = 0.018) and for DPIs (81% vs. 35%; p = 0.010). CONCLUSIONS: In this sample, the most common errors in the use of inhalers were not exhaling prior to inhalation, not performing a breath-hold after inhalation, and not shaking the MDI prior to use. Special attention should be given to education regarding inhaler techniques for patients of lower socioeconomic status and with less formal education, as well as for those of advanced age, because those populations are at a greater risk of committing errors in their use of inhalers. PMID:25410839

  4. Seroprevalence of hepatitis a antibodies in a group of normal and Down syndrome children in Porto Alegre, southern Brazil.

    PubMed

    Ferreira, Cristina Targa; Leite, Júlio César; Tanaguchi, Adriano Nori R; Vieira, Sandra Maria G; Pereira-Lima, Jorge; da Silveira, Themis Reverbel

    2002-10-01

    The high incidence of Hepatitis A and B in institutionalized patients with Down Syndrome (DS) is not fully understood. Under poor hygienic conditions, immunological alterations might predispose individuals to these infections. Sixty three DS children between 1 and 12 years old living at home with their families were examined for anti-HAV and compared to age-matched controls (64 healthy children). This cross-sectional study was carried out from May 1999 to April 2000 at the Hospital de Clínicas of Porto Alegre, southern Brazil. Groups were compared in terms of age, sex, skin color, and family income (> R$ 500 and < R $ 500/month) by the chi-square test, with Yates' correction and for the prevalence of anti-HAV (Fisher's exact test). In the DS group (n=63), the mean age was 4.4 +/- 3.3 years, 94% of the patients were white and 51% were female. Family income was < or = R$ 500/month in 40 cases (63%). In the control group (n=64), the mean age was 4.8 +/- 2.7 years, 81% of the patients were white and 56% were female. Family income was < or = R$ 500 in 20 patients (31%). DS children's families had a significantly lower income (P<0.0005). In the DS group there were 6 positive (9.5%) anti-HAV cases, and all came from low-income families (less than R$ 500/ month). In the control group, 3 cases (4.7%) were positive for anti-HAV (two were from a low-income family and one was from a higher income family). These differences were not significant. Our data indicate that Hepatitis A is not a special risk for mentally retarded DS outpatients, even in a developing country like Brazil.

  5. Comparative assessment of genetic and morphological variation at an extensive hybrid zone between two wild cats in southern Brazil.

    PubMed

    Trigo, Tatiane C; Tirelli, Flávia P; de Freitas, Thales R O; Eizirik, Eduardo

    2014-01-01

    Increased attention towards the Neotropical cats Leopardus guttulus and L. geoffroyi was prompted after genetic studies identified the occurrence of extensive hybridization between them at their geographic contact zone in southern Brazil. This is a region where two biomes intersect, each of which is associated with one of the hybridizing species (Atlantic Forest with L. guttulus and Pampas with L. geoffroyi). In this study, we conducted in-depth analyses of multiple molecular markers aiming to characterize the magnitude and spatial structure of this hybrid zone. We also performed a morphological assessment of these species, aiming to test their phenotypic differentiation at the contact zone, as well as the correlation between morphological features and the admixture status of the individuals. We found strong evidence for extensive and complex hybridization, with at least 40% of the individuals sampled in Rio Grande do Sul state (southernmost Brazil) identified as hybrids resulting from post-F1 generations. Despite such a high level of hybridization, samples collected in this state still comprised two recognizable clusters (genetically and morphologically). Genetically pure individuals were sampled mainly in regions farther from the contact zone, while hybrids concentrated in a central region (exactly at the interface between the two biomes). The morphological data set also revealed a strong spatial structure, which was correlated with the molecular results but displayed an even more marked separation between the clusters. Hybrids often did not present intermediate body sizes and could not be clearly distinguished morphologically from the parental forms. This observation suggests that some selective pressure may be acting on the hybrids, limiting their dispersal away from the hybrid zone and perhaps favoring genomic combinations that maintain adaptive phenotypic features of one or the other parental species.

  6. Microbial quality of irrigation water used in leafy green production in Southern Brazil and its relationship with produce safety.

    PubMed

    Decol, Luana Tombini; Casarin, Letícia Sopeña; Hessel, Claudia Titze; Batista, Ana Carolina Fösch; Allende, Ana; Tondo, Eduardo César

    2017-08-01

    Irrigation water has been recognized as an important microbial risk factor for fruits and vegetables in many production areas, but there is still a lack of information about how the microbiological quality of different irrigation water sources and climatic conditions influence the safety of vegetables produced in Brazil. This study evaluated the distribution of generic E. coli and the prevalence of E. coli O157:H7 in two different water sources (ponds and streams bordering farmlands and urban areas) used for irrigation and on commercially produced lettuces in Southern Brazil. We also evaluated the effect of agricultural factors and meteorological conditions in the potential contamination of water and produce samples. A longitudinal study was conducted on four farms during a year (July 2014 to August 2015). The results showed generic E. coli prevalence of 84.8% and 38.3% in irrigation water samples and on lettuces, respectively, indicating irrigation water as an important source of contamination of lettuces. No significant differences were detected in the counts of E. coli between the two different surface water sources. The climatic conditions, particularly rainfall and environmental temperature, have influenced the high concentration of E. coli. The highest loads of E. coli in irrigation water and on lettuces were found during the warmest time of the year. E. coli O157:H7 was detected by qualitative polymerase chain reaction (qPCR) in 13 water samples but only 4 were confirmed by isolation in culture media. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. MICA diversity and linkage disequilibrium with HLA-B alleles in renal-transplant candidates in southern Brazil.

    PubMed

    Yamakawa, Roger Haruki; Saito, Patrícia Keiko; Gelmini, Geórgia Fernanda; da Silva, José Samuel; Bicalho, Maria da Graça; Borelli, Sueli Donizete

    2017-01-01

    The major histocompatibility complex (MHC) class I chain-related gene A (MICA) is located centromerically to the human leukocyte antigen (HLA)-B. The short distance between these loci in the MHC indicates the presence of linkage disequilibrium (LD). Similarly to the HLA, the MICA is highly polymorphic, and this polymorphism has not been well documented in different populations. In this study, we estimated the allelic frequencies of MICA and the linkage disequilibrium with HLA-B alleles in 346 renal-transplant candidates in southern Brazil. MICA and HLA were typed using the polymerase chain reaction-sequence-specific primer method (PCR-SSO), combined with the Luminex technology. A total of 19 MICA allele groups were identified. The most frequent allele groups were MICA*008 (21.6%), MICA*002 (17.0%) and MICA*004 (14.8%). The most common haplotypes were MICA*009-B*51 (7.8%), MICA*004-B*44 (6.06%) and MICA*002-B*35 (5.63%). As expected from the proximity of the MICA and HLA-B loci, most haplotypes showed strong LD. Renal patients and healthy subjects in the same region of Brazil showed statistically significant differences in their MICA polymorphisms. The MICA*027 allele group was more frequent in renal patients (Pc = 0.018, OR: 3.421, 95% CI: 1.516-7.722), while the MICA*019 allele group was more frequent in healthy subjects (Pc = 0.001, OR: 0.027, 95% CI: 0.002-0.469). This study provided information on the distribution of MICA polymorphisms and linkage disequilibrium with HLA-B alleles in Brazilian renal-transplant candidates. This information should help to determine the mechanisms of susceptibility to different diseases in patients with chronic kidney disease, and to elucidate the mechanisms involved in allograft rejection associated with MICA polymorphisms in a Brazilian population.

  8. Comparative Assessment of Genetic and Morphological Variation at an Extensive Hybrid Zone between Two Wild Cats in Southern Brazil

    PubMed Central

    Trigo, Tatiane C.; Tirelli, Flávia P.; de Freitas, Thales R. O.; Eizirik, Eduardo

    2014-01-01

    Increased attention towards the Neotropical cats Leopardus guttulus and L. geoffroyi was prompted after genetic studies identified the occurrence of extensive hybridization between them at their geographic contact zone in southern Brazil. This is a region where two biomes intersect, each of which is associated with one of the hybridizing species (Atlantic Forest with L. guttulus and Pampas with L. geoffroyi). In this study, we conducted in-depth analyses of multiple molecular markers aiming to characterize the magnitude and spatial structure of this hybrid zone. We also performed a morphological assessment of these species, aiming to test their phenotypic differentiation at the contact zone, as well as the correlation between morphological features and the admixture status of the individuals. We found strong evidence for extensive and complex hybridization, with at least 40% of the individuals sampled in Rio Grande do Sul state (southernmost Brazil) identified as hybrids resulting from post-F1 generations. Despite such a high level of hybridization, samples collected in this state still comprised two recognizable clusters (genetically and morphologically). Genetically pure individuals were sampled mainly in regions farther from the contact zone, while hybrids concentrated in a central region (exactly at the interface between the two biomes). The morphological data set also revealed a strong spatial structure, which was correlated with the molecular results but displayed an even more marked separation between the clusters. Hybrids often did not present intermediate body sizes and could not be clearly distinguished morphologically from the parental forms. This observation suggests that some selective pressure may be acting on the hybrids, limiting their dispersal away from the hybrid zone and perhaps favoring genomic combinations that maintain adaptive phenotypic features of one or the other parental species. PMID:25250657

  9. Use of the palm Euterpe edulis martius in landscape units managed by migrants of German origin in Southern Brazil

    PubMed Central

    2013-01-01

    Background People influence their environments through the manipulation of landscapes and species. Human influence on the landscape may lead to the development of differentiated landscape units that originate from past use and may be related to the presence of certain species. This study investigated the presence of the palm Euterpe edulis and its current and past importance in landscape units established by a community of German descendants located in southern Brazil. The objectives of this study were to characterize the use of the species, to identify the importance of E.edulis for the German immigrant community, to identify past and current uses of E.edulis, to describe the historical use of the landscape, and lastly, to identify landscape units in which E.edulis is found. Methods The researched community is composed of people of German descent residing in southern Brazil. A variety of research tools were used to achieve the objectives of the research. Semi-structured interviews and free-listings were conducted in all family units. The interviews focused on groups of people in the community who had current or historical connection with the species. Group workshops and guided tours were conducted to identify different landscape units. The data were analyzed using descriptive statistics, use-value index, citation frequency, salience index, and informant perception analysis. Results Over the historical period studied, the community demonstrated changes with respect to economic activities. These changes are reflected in the transformation of the landscape. The species E.edulis was and still is very important for people in the community; its importance is reflected in its high use value, citation frequency and salience. The species is found within various landscape units in the community as well as in homegardens and in secondary forests. Conclusions The landscape heterogeneity of this community is influenced by changes in economic activities and by the relationship

  10. Nine thousand years of upper montane soil/vegetation dynamics from the summit of Caratuva Peak, Southern Brazil

    NASA Astrophysics Data System (ADS)

    Scheer, Maurício B.; Pereira, Nuno Veríssimo; Behling, Hermann; Curcio, Gustavo R.; Roderjan, Carlos V.

    2014-12-01

    Biodiversity loss, climate change, and increased freshwater consumption are some of the main environmental problems on Earth. Mountain ecosystems can reduce these threats by providing several positive influences, such as the maintenance of biodiversity, water regulation, and carbon storage, amongst others. The knowledge of the history of these environments and their response to climate change is very important for management, conservation, and environmental monitoring programs. The genesis of the soil organic matter of the current upper montane vegetation remains unclear and seems to be quite variable depending on location. Some upper montane sites in the very extensive coastal Sea Mountain Range present considerable organic matter from the late Pleistocene and other from only the Holocene. Our study was carried out on three soil profiles (two cores in grassland and one in forest) on the Caratuva Peak of the Serra do Ibitiraquire (a sub-range of Sea Mountain Range - Serra do Mar) in Southern Brazil. The δ13C isotopic analyses of organic matter in soil horizons were conducted to detect whether C3 or C4 plants dominated the past communities. Complementarily, we performed a pollen analysis and 14C dating of the humin fraction to obtain the age of the studied horizons. Except for a short and probably drier period (between 6000 and 4500 cal yr BP), C3 plants, including ombrophilous grasses and trees, have dominated the highlands of the Caratuva Peak (Pico Caratuva), as well as the other uppermost summits of the Serra do Ibitiraquire, since around 9000 cal yr BP. The Caratuva region represents a landscape of high altitude grasslands (campos de altitude altomontanos or campos altomontanos) and upper montane rain/cloud forests with soils that most likely contain some organic matter from the late Pleistocene, as has been reported in Southern and Southeastern Brazil for other sites. However, our results indicate that the studied deposits (near the summit) are from the early

  11. Influence of KIR genes and their HLA ligands in susceptibility to dengue in a population from southern Brazil.

    PubMed

    Beltrame, L M; Sell, A M; Moliterno, R A; Clementino, S L; Cardozo, D M; Dalalio, M M; Fonzar, U J; Visentainer, J E

    2013-12-01

    Killer cell immunoglobulin-like receptors (KIR) form a group of regulatory molecules that specifically recognise human leukocyte antigen (HLA) class I molecules, modulating the cytolytic activity of natural killer cells. The purpose of this study was to investigate the influence of KIR genes and their class I HLA ligands in susceptibility to dengue fever in a population from southern Brazil through a case-control study. One hundred four subjects with confirmed diagnoses of dengue participated in this study, along with a control group of 172 individuals from the same geographic area. HLA and KIR genotyping was performed by polymerase chain reaction with sequence-specific oligonucleotide probes (PCR-SSOP) and with sequence-specific primer (PCR-SSP) techniques, respectively. Data analysis showed significant differences for the KIR2DS1 (54.8% vs 40.7%, P = 0.03), KIR2DS5 (50.0% vs 36.0%, P = 0.03) and KIR2DL5 (76.0% vs 56.4%, P = 0.001) genes. With regard to KIR-ligand pairs, positive associations with dengue were observed in KIR3DS1-Bw4 (45.2% vs 29.7%, P = 0.01), KIR3DL1-Bw4 (80.7% vs 65.1%, P < 0.001), KIR2DL1-C2 (75.0% vs 62.2%, P = 0.03) and KIR2DS1-C2 (40.4% vs 25.6%, P = 0.01) interactions, and a negative association in KIR2DL3-C1/C1 (18.2% vs 33.1%, P = 0.01). Furthermore, the analysis of KIR haplogroups showed a possible protective factor against dengue fever in individuals with the AA genotype. Taken together, these results suggest the existence of genetic predisposition to dengue fever in the population from southern Brazil.

  12. Increased incidence of choroid plexus carcinoma due to the germline TP53 R337H mutation in southern Brazil.

    PubMed

    Custodio, Gislaine; Taques, Guilherme R; Figueiredo, Bonald C; Gugelmin, Elizabeth S; Oliveira Figueiredo, Mirna M; Watanabe, Flora; Pontarolo, Roberto; Lalli, Enzo; Torres, Luiz Fernando Bleggi

    2011-03-22

    Choroid plexus carcinomas (CPC) are rare tumors predominantly found in children. Given the high frequency of the germline R337H mutation in the TP53 gene in southern Brazil, we have evaluated the frequency of the R337H mutation in families with CPC in children. The present series included 29 patients that were admitted to the same institution from 1992 to 2010, including 22 children with CPC (0.08-13.6 years of age at diagnosis) and 7 children with papilloma of the choroid plexus (Pp; 0.5-9.8 years of age). Surgical resection was possible in 28 children. Blood and/or tumor DNA was extracted and analyzed using PCR-RFLP and results were confirmed by sequencing 240 bp of the TP53 exon 10. The patients, all parents, and some relatives submitted samples for blood DNA analysis. In addition, we have also examined the presence of the mutation in DNA from paraffin-embedded tumor samples to evaluate loss of heterozygosity. We found 63.3% (14/22) of the CPC patients positive for the germline R337H mutation; CPC samples were either heterozygous (n = 7), lost only the wild-type (n = 4), or only the R337H copy (n = 2). One CPC sample was not available. All Pp cases (7/7, 100%) were negative for R337H. Cure (>5 years survival free of disease) was observed in 18.1% of the CPC cases with the R337H mutation (2/11), 71.4% of the Pp (5/7), and 25% of CPC cases negative for the R337H mutation (2/8). Family history of cancer (with 2 or more cancer cases) was exclusively identified on the parental side segregating the R337H mutation, and 50% (7/14) of them were compatible with Li-Fraumeni-like syndrome. Our results show for the first time that the R337H TP53 mutation is responsible for 63% of the CPC cases in children, suggesting a higher incidence of CPC in southern Brazil.

  13. Potential of carbon accumulation in no-till soils with intensive use and cover crops in southern Brazil.

    PubMed

    Amado, Telmo Jorge Carneiro; Bayer, Cimélio; Conceição, Paulo Cesar; Spagnollo, Evandro; de Campos, Ben-Hur Costa; da Veiga, Milton

    2006-01-01

    The area under no-till (NT) in Brazil reached 22 million ha in 2004-2005, of which approximately 45% was located in the southern states. From the 1970s to the mid-1980s, this region was a source of carbon dioxide to the atmosphere due to decrease of soil carbon (C) stocks and high consumption of fuel by intensive tillage. Since then, NT has partially restored the soil C lost and reduced the consumption of fossil fuels. To assess the potential of C accumulation in NT soils, four long-term experiments (7-19 yr) in subtropical soils (Paleudult, Paleudalf, and Hapludox) varying in soil texture (87-760 g kg(-1) of clay) in agroecologic southern Brazil zones (central region, northwest basaltic plateau in Rio Grande Sul, and west basaltic plateau in Santa Catarina) and with different cropping systems (soybean and maize) were investigated. The lability of soil organic matter (SOM) was calculated as the ratio of total organic carbon (TOC) to particulate organic carbon (POC), and the role of physical protection on stability of SOM was evaluated. In general, TOC and POC stocks in native grass correlated closely with clay content. Conversely, there was no clear effect of soil texture on C accumulation rates in NT soils, which ranged from 0.12 to 0.59 Mg ha(-1) yr(-1). The C accumulation was higher in NT than in conventional-till (CT) soils. The legume cover crops pigeon pea [Cajanus cajan (L.) Millsp] and velvet beans (Stizolobium cinereum Piper & Tracy) in NT maize cropping systems had the highest C accumulation rates (0.38-0.59 Mg ha(-1) yr(-1)). The intensive cropping systems also were effective in increasing the C accumulation rates in NT soils (0.25-0.34 Mg ha(-1) yr(-1)) when compared to the double-crop system used by farmers. These results stress the role of N fixation in improving the tropical and subtropical cropping systems. The physical protection of SOM within soil aggregates was an important mechanism of C accumulation in the sandy clay loam Paleudult under NT

  14. Long-term vegetation, climate and ocean dynamics inferred from a 73,500 years old marine sediment core (GeoB2107-3) off southern Brazil

    NASA Astrophysics Data System (ADS)

    Gu, Fang; Zonneveld, Karin A. F.; Chiessi, Cristiano M.; Arz, Helge W.; Pätzold, Jürgen; Behling, Hermann

    2017-09-01

    Long-term changes in vegetation and climate of southern Brazil, as well as ocean dynamics of the adjacent South Atlantic, were studied by analyses of pollen, spores and organic-walled dinoflagellate cysts (dinocysts) in marine sediment core GeoB2107-3 collected offshore southern Brazil covering the last 73.5 cal kyr BP. The pollen record indicates that grasslands were much more frequent in the landscapes of southern Brazil during the last glacial period if compared to the late Holocene, reflecting relatively colder and/or less humid climatic conditions. Patches of forest occurred in the lowlands and probably also on the exposed continental shelf that was mainly covered by salt marshes. Interestingly, drought-susceptible Araucaria trees were frequent in the highlands (with a similar abundance as during the late Holocene) until 65 cal kyr BP, but were rare during the following glacial period. Atlantic rainforest was present in the northern lowlands of southern Brazil during the recorded last glacial period, but was strongly reduced from 38.5 until 13.0 cal kyr BP. The reduction was probably controlled by colder and/or less humid climatic conditions. Atlantic rainforest expanded to the south since the Lateglacial period, while Araucaria forests advanced in the highlands only during the late Holocene. Dinocysts data indicate that the Brazil Current (BC) with its warm, salty and nutrient-poor waters influenced the study area throughout the investigated period. However, variations in the proportion of dinocyst taxa indicating an eutrophic environment reflect the input of nutrients transported mainly by the Brazilian Coastal Current (BCC) and partly discharged by the Rio Itajaí (the major river closest to the core site). This was strongly related to changes in sea level. A stronger influence of the BCC with nutrient rich waters occurred during Marine Isotope Stage (MIS) 4 and in particular during the late MIS 3 and MIS 2 under low sea level. Evidence of Nothofagus pollen

  15. [Mosquitos of peri and extradomiciliary environments in the southern region of Brazil].

    PubMed

    Teodoro, U; Guilherme, A L; Lozovei, A L; La Salvia Filho, V; Sampaio, A A; Spinosa, R P; Ferreira, M E; Barbosa, O C; de Lima, E M

    1994-04-01

    Mosquitoes were collected on Sonho Real farm, Querência do Norte county, Paraná State, Brazil, using human bait and Falcão traps between June 1989 and May 1990. The fauna composition, monthly density, hours of major density, human attraction and presence of mosquitoes in domestic animal shelters were investigated. 5,923 mosquitoes of the genera Aedes, Aedomyia, Anopheles, Coquillettidea, Culex, Mansonia, Psorophora, Sabethes and Uranotaenia were collected. 33 species of mosquitoes were identified and among them Aedes scapularis, Anopheles albitarsis, Aedomyia squamipennis, Coquillettidea lynchi, Mansonia titillans e Coquillettidea venezuelensis were predominant. All these species were captured mainly on human bait, except Aedomyia squamipennis that was captured in domestic animal shelters. With regard to all the mosquitoes captured (5,923), their major period of activity was between 18 and 19 hours and April was the month of greatest density.

  16. [Childbirth practices and challenges for humanization of care in two public hospitals in Southern Brazil].

    PubMed

    Nagahama, Elizabeth Eriko Ishida; Santiago, Silvia Maria

    2008-08-01

    The aim of this study was to characterize hospital care for childbirth in two hospitals affiliated with the Unified National Health System in Maringá, Paraná, Brazil, and identify both obstacles and facilitating factors for the implementation of humanized care, based on women's perception of the care received. This was an exploratory and descriptive study with a cross-sectional design, analyzing hospital patient charts and interviews with 569 women who gave birth at the two hospitals from March 2005 to February 2006. Hospital care was characterized on the basis of WHO quality-of-care guidelines for labor and delivery. The data pointed to a healthcare model marked by the hygienist legacy in physician-centered hospital protocols and professional practices. Institutional factors, identified as difficulties in institutional and infrastructure organization, hospital protocols, and health professionals' individual practices and stances, denote barriers that jointly hinder the implementation of a humanized model for childbirth care.

  17. Hepatitis E Virus in Surface Water, Sediments, and Pork Products Marketed in Southern Brazil.

    PubMed

    Heldt, F H; Staggmeier, R; Gularte, J S; Demoliner, M; Henzel, A; Spilki, F R

    2016-09-01

    Hepatitis E virus (HEV) is an emerging causative agent of food and waterborne hepatitis in human beings. HEV circulates among human populations and swine herds, and may be found in water contaminated by swine feces, as well as in pork. In the present study, 68 sediment samples and 250 water samples collected from the Sinos River tributaries, as well as 50 samples of pork products (pâté and blood sausage) marketed in the Sinos River watershed region, Brazil, were tested for the presence of HEV genome. Reverse-transcriptase polymerase chain reaction followed by nucleotide sequencing was used for detection and characterization of HEV genomes. Overall, 36 % of food samples tested positive for HEV (genotype 3). No sediment or water samples were positive. These results suggest that contaminated pork products may be a source of HEV infection within this region and indicate a need for better monitoring of food safety and swine herds.

  18. [Weight and height validation for diagnosis of adult nutritional status in southern Brazil].

    PubMed

    Silveira, Erika Aparecida da; Araújo, Cora Luíza; Gigante, Denise Petrucci; Barros, Aluisio J D; Lima, Maurício Silva de

    2005-01-01

    This study evaluated the accuracy of body mass index (BMI) based on self-reported weight and height for predicting adult nutritional status. In a cross-sectional study of 3,934 adults (> 20 years) in Pelotas, Rio Grande do Sul, Brazil, a sub-sample of 140 individuals was drawn and weight and height were measured. From the comparison between "measured" and "reported" BMI, the average reported BMI error was estimated and the associated factors were identified. Regardless of nutritional status, women underestimated their "reported" BMI, while in men this information was accurate. Among women, age and income were associated with underestimated BMI in a multivariate analysis. Thus, women over 50 and with lower income underestimated BMI by more than 2 kg/m2. The use of "reported" BMI to predict adult nutritional status can underestimate prevalence of obesity and overestimate that of overweight in women. Correction minimizes this kind of bias, thereby making the data more accurate.

  19. Foraminifera as indicators of marine pollutant contamination on the inner continental shelf of southern Brazil.

    PubMed

    Eichler, Patricia P B; Eichler, Beatriz B; Gupta, Barun Sen; Rodrigues, André Rösch

    2012-01-01

    Analyses of living foraminiferal and environmental parameters near an outfall at Mar Grosso Beach (Laguna, SC, Brazil) demonstrate its usefulness as indicators of domestic sewage pollution. The low species diversity may be due to sand accumulation in the central part. Higher diversity was noted closer to the mouth of Laguna estuarine system where reduced salinity and higher temperatures indicate freshwater influence, suggesting a relationship between increased diversity and greater availability of terrestrial food. On the basis of foraminiferal diversity and average coliform count the higher values are closer to the mouth of the estuarine system and under the influence of the outfall. Due to the effect of local hydrodynamics, the particulate organic waste derived from the outfall does not settle down locally, and thus, do not accumulate nearby. Our hypothesis is that the fine material derived from the outfall is accumulating on the southwestern and northwestern parts of the beach.

  20. Community structure of helminth parasites of the "Cururu" toad, Rhinella icterica (Anura: Bufonidae) from southern Brazil.

    PubMed

    Dos Santos, Viviane Gularte Tavares; Amato, Suzana B; Borges-Martins, Márcio

    2013-03-01

    Sixty specimens of the "cururu" toad, Rhinella icterica (Spix 1824) (Bufonidae), were collected in Campo Belo do Sul, State of Santa Catarina, Brazil, between May 2009 and January 2011, and were examined for the presence of helminth parasites. Nine species of adult helminths were found: Catadiscus cohni, Rudolphitrema rudolphii, Cylindrotaenia sp., Rhabdias fuelleborni, Strongyloides sp., Cosmocerca rara, Cosmocerca brasiliensis, Aplectana elenae, and Oxyascaris sp., in addition to an unidentified adult nematode species. Females of cosmocercid nematodes, proteocephalan plerocercoid, and acanthocephalan cystacanth were found but not identified for lack absolute of taxonomic characters. The sex of the anurans had no influence on prevalence, abundance, and richness of helminth species. Length and body mass of hosts did not influence the prevalence and richness of helminths, while the abundance of R. fuelleborni was significantly correlated with both parameters.

  1. Evaluation of waterborne exposure to oil spill 5 years after an accident in Southern Brazil.

    PubMed

    Silva, C A; Oliveira Ribeiro, C A; Katsumiti, A; Araújo, M L P; Zandoná, E M; Costa Silva, G P; Maschio, J; Roche, H; Silva de Assis, H C

    2009-02-01

    Fish (Astyanax sp.) were exposed to water samples from the Arroio Saldanha, locality of an oil spill, and the Barigui River (upstream and downstream of the confluence of the river and Arroio Saldanha), Brazil. Histopathological and biochemical biomarkers, as acetylcholinesterase (AchE), ethoxyresorufin-O-deethylase (EROD), catalase (CAT), glutathione-S-transferase (GST) activities, lipid peroxidation (LPO) levels, and amounts of polynuclear aromatic hydrocarbon residues (PAHs) in bile were evaluated. PAHs, metals, and surfactants were investigated in the water samples. CAT and GST activities increased in the Arroio Saldanha sample, whereas CAT activity and LPO levels were increased in the upstream and downstream sites. The results suggested a toxic action of the free radicals and disturbance of the antioxidant defense mechanisms. However, the EROD and AchE activities were not affected. Histopathology studies showed severe lesions. The oil is still bioavailable to biota, moreover, other pollution sources continue to affect the water in the Barigui River.

  2. Description of three new species of Eumida Malmgren, 1865 (Phyllodocidae, Annelida) from Southern and Southeastern Brazil.

    PubMed

    De Oliveira, Verônica Maria; Eibye-Jacobsen, Danny; Lana, Paulo Da Cunha

    2015-05-18

    We describe three new species of Eumida from shallow estuarine and shelf bottoms from south and southeastern Brazil as part of a regional survey of the family Phyllodocidae. Previous regional records of Eumida sanguinea (Örsted, 1843) are herein referred to Eumida dracodermica sp. nov. The two species differ in the size of the prostomium, the distribution of micropapillae on the proboscis, and the shape of the dorsal cirri. Eumida macrophthalma sp. nov. has much larger eyes than the other two species described herein. The dorsal cirri on anterior median segments are rounded and on median and posterior segments they are asymmetrical and cordiform, longer than the dorsal cirri in Eumida dracodermica sp. nov. The lanceolate median dorsal cirri in Eumida delicata sp. nov. are only shared with E. ockelmanni Eibye-Jacobsen, 1987. It is also possible to diagnose the three species by their dorsal whitish pigmentation patterns on the first segments of living or well conserved animals.

  3. Characterization of Toxoplasma gondii isolates from herds of sheep in southern Brazil reveals the archetypal type II genotype and new non-archetypal genotypes.

    PubMed

    da Silva Ramos, Tatiana; de Jesus Pena, Hilda Fátima; Dos Santos Junior, Alceu Gonçalves; de Faria Santos, Laura Maria Jorge; Cademartori, Beatris Gonzales; Oliveira, Solange; Gennari, Solange Maria; da Silva Ramos Rocha, Andréa; da Rosa Farias, Nara Amélia

    2017-03-23

    Recent studies have demonstrated that, in Brazil and South America, strains of Toxoplasma gondii are often genotypically and biologically different from those found in countries on other continents. The objective of this study was to genotypically characterize T. gondii isolates from naturally infected sheep in herds in the southern region of the state of Rio Grande do Sul, Brazil, by means of the polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP). Five T. gondii isolates obtained from sheep in five municipalities in the state of Rio Grande do Sul were used. Application of multilocus PCR-RFLP multilocus using 12 genetic markers (SAG1, 5'3' SAG2, alt. SAG2, SAG3, BTUB, c22-8, c29-2, GRA6, L358, PK1, APICO and CS3) revealed four different genotypes in the five isolates studied: clonal type II (TgOvBrRS4), type BrIV (TgOvBrRS2 and TgOvBrRS3) and two new non-archetypal genotypes, ToxoDB-RFLP#270 and #271 (TgOvBrRS1 and TgOvBrRS5, respectively). The genotype structure found in the T. gondii isolates from naturally infected sheep in the southern region of Brazil was revealed to have high diversity. This study confirms the presence of rare circulation of the clonal type II genotype in Brazil.

  4. Shortage and underutilization of psychiatric beds in southern Brazil: independent data of Brazilian mental health reform.

    PubMed

    Candiago, Rafael Henriques; Saraiva, Sergio da Silva; Gonçalves, Veralice; Belmonte-de-Abreu, Paulo

    2011-05-01

    Mental health policies throughout the world are being subjected to several changes due to increased pressure from the public and from health administrators. Mental health policies in the developing world experienced changes following advice and consultation from the World Health Organization (WHO). This was the case with Brazil, which enacted several laws and policies affecting community care and the closure of beds in psychiatric hospitals (the deinstitutionalization movement). Rio Grande do Sul, the southeast state in Brazil, adopted this policy in 1992, but still suffers from a shortage of psychiatric beds in both general hospitals (GHs) and psychiatric hospitals (PHs), despite advances in the provision of community care. As a result, Rio Grande do Sul (RS) can be studied as an example of "what actually happens" in mental health care in the developing world. The study analyzed online administrative data from 2000 to 2007 for three main parameters of mental health care (i.e. hospital use rate, length of stay, and hospital bed capacity). These were used to evaluate differences in psychiatric care among GHs and PHs. The number of existing and required psychiatric beds in RS was also calculated. GHs had very low hospital use rates, while PHs had a very high length of stay (200% higher than GH). The number of admissions to GHs grew by 170% over the 8 year period, and psychiatric bed availability was 1.5 per 10,000 inhabitants. The study provided evidence for the need of 30-50% more psychiatric beds in GHs. Also, additional staff training is needed to increase use rates in GHs and decrease the length of stay in PHs. Additional studies covering national data must be carried out to assess the extent of these problem in other Brazilian mental health care services.

  5. Mapping flood prone areas in southern Brazil: a combination of frequency analysis, HAND algorithm and remote sensing methods

    NASA Astrophysics Data System (ADS)

    Fabris Goerl, Roberto; Borges Chaffe, Pedro Luiz; Marcel Pellerin, Joel Robert; Altamirano Flores, Juan Antonio; Josina Abreu, Janete; Speckhann, Gustavo Andrei; Mattos Sanchez, Gerly

    2015-04-01

    Floods disaster damages several people around the world. There is a worldwide increasing trend of natural disasters frequency and their negative impacts related to the population growth and high urbanization in natural hazards zones. In Santa Catarina state, such as almost all southern Brazilian territory, floods are a frequent hydrological disaster. In this context, flood prone areas map is a important tool to avoid the construction of new settlements in non-urbanizations areas. The present work aimed to map flood prone areas in Palhoça City, Southern Brazil combining high resolution digital elevations data, remote sensing information, frequency analysis and High Above Nearest Drainage (HAND) algorithm. We used 17 years of daily discharge and stage data to calculate flood probability and return period. Remote Sensing (RS) with CBERS HRC image with 2,7m resolution was used. This image was taken one day after one flood occurrence and a band difference was used to extract the flood extent. HAND using DEM to calculate the altimetric difference between channel pixel and adjacent terrain values. All morphometric attributes used in HAND were extracted directly from the high resolution DEM (1m). Through CBERS image areas where flood level was higher than 0.5m were mapped. There is some kind of uncertain in establish HAND classes, since only distance to the channel was take in account. Thus, using other hydrological or spatial information can reduce this uncertain. To elaborate the final flood prone map, all this methods were combined. This map was classified in three main classes based on return period. It was notices that there is a strong spatial correlation between high susceptibility flood areas and geomorphological features like floodplains and Holocene beach ridges, places where water table emerges frequently. The final map was classified using three different colors (red, yellow and green) related to high, medium an law susceptibility flood areas. This mapping

  6. Genetic identification of Rickettsia sp. strain Atlantic rainforest in an endemic area of a mild spotted fever in Rio Grande do Sul state, Southern Brazil.

    PubMed

    Figueiredo Vizzoni, Vinicius; Barbosa Silva, Arannadia; Medeiros Cardoso, Karen; Barbosa Dos Santos, Fernanda; Stenzel, Barbara; Amorim, Marinete; Vilges de Oliveira, Stefan; Salles Gazeta, Gilberto

    2016-10-01

    Rickettsia sp. strain Atlantic rainforest causes a less severe rickettsiosis, with two cases confirmed until now. The tick species Amblyomma ovale is appointed as the main vector of this bacterium. The southern region of Brazil has reported patients with spotted fever who have milder symptoms. In 2013, during an investigation of rickettsiosis cases, an A. ovale tick was found attached to a man in an area where there were two cases. The parasite was processed for molecular analysis and the rickettsial infection was confirmed based on phylogenetic analysis of genes ompA, ompB and geneD (sca4). In the present study the human pathogenic Rickettsia sp. strain Atlantic rainforest was identified in the state of Rio Grande do Sul, Southern Brazil. Since A. ovale, its main vector, is found frequently parasitizing dogs, animals that can cross international borders freely in southern Brazil, this bacteria can bring major concerns in terms of public health. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Implication of Tityus apiacas (Lourenco, 2002) in scorpion envenomations in the Southern Amazon border, Brazil.

    PubMed

    Silva, Bruna Andressa Jung da; Fé, Nelson Ferreira; Gomes, André Alexandre Dos Santos; Souza, Anderson da Silva; Sachett, Jacqueline de Almeida Gonçalves; Fan, Hui Wen; Melo, Gisely Cardoso de; Monteiro, Wuelton Marcelo

    2017-01-01

    Herein, four cases of scorpion stings caused by Tityus apiacas recorded from the municipality of Apuí, in the southern region of the Brazilian Amazon, are described. Patients showed systemic clinical manifestations, described as unusual, involuntary, and generalized tingling and numbness, reported by patients as an electric shock sensation, lasting up to 24 hours after the sting. All patients described local pain and sensation, along with other clinical symptoms including local edema and erythema. Systemic manifestations were not life threatening. Antivenom therapy was administered to all patients, who were discharged without complaints.

  8. Hepatitis C virus prevalence among an immigrant community to the southern Amazon, Brazil.

    PubMed

    Souto, F J; Fontes, C J; Martelli, C M; Turchi, M D; Martins, R M; Andrade, A L

    1999-01-01

    A community-based random survey was conducted in a southern Brazilian Amazonian county aiming to investigate hepatitis C virus (HCV) infection prevalence and the association of demographic variables and lifestyle behaviours. Seven hundred eighty individuals were serologically screened with a third generation enzyme-linked immunosorbent assay to detect anti-HCV antibodies between 1994/1995. Positive samples were retested for confirmation with a line immunoassay (LIA, Inno-LIA HCV Ab III). Most of these subjects were low income and came from southern Brazilian states (65.8). Two point four percent (IC 95% 1.2%- 4.6%) of the subjects had LIA-confirmed anti-HCV antibodies reactivity. The age-specific prevalence of HCV antibodies slightly increased with age, with the highest prevalence after the age of 40 years. The results of multivariate analysis indicate a strong association between HCV antibodies and previous surgery and history of intravenous drug use. There were no apparent association with gender, hepatitis B virus markers, blood transfusion, and sexual activity. Mean time living in Amazon did not differ between confirmed and negative anti-HCV individuals. The present data point out an intermediate endemicity of HCV infection among this immigrant community to the Amazon region and that few HCV infected participants presented known risk factors.

  9. Presence of Zea luxurians (Durieu and Ascherson) Bird in Southern Brazil: Implications for the Conservation of Wild Relatives of Maize.

    PubMed

    Silva, Natália Carolina de Almeida; Vidal, Rafael; Costa, Flaviane Malaquias; Vaio, Magdalena; Ogliari, Juliana Bernardi

    2015-01-01

    Records of the occurrence of wild relatives of maize in South American lowlands are unprecedented, especially in sympatric coexistence with landraces. This fact is relevant, because regions of occurrence of wild relatives of cultivated plants should be a priority for conservation, even if they do not correspond to the center of origin of the species. The aim of this study was to identify and characterize the wild relatives of maize in the Far West of Santa Catarina, southern Brazil. Therefore, phenotypic characterization was performed for five populations, based on 22 morphological traits deemed as fundamental for classifying the species of the genus Zea, and validated through the characterization of chromosomal knobs of two populations. The occurrence and distribution of teosinte populations were described through semi-structured interviews applied to a sample of 305 farmers. A total of 136 teosinte populations were identified; 75% of them occur spontaneously, 17% are cultivated populations, and 8% occur both ways, for the same farm. Populations that were characterized morphologically had trapezoidal fruits mostly, upright tassel branch (4-18), non-prominent main branch and glabrous glumes, with two protruding outer ribs and 8 inner ribs, on average. Cytogenetic analysis identified 10 pairs of homologous chromosomes (2n = 20) with 26 knobs, located in the terminal region of all chromosomes. The similarity of these results with the information reported in the literature indicates that the five populations of wild relatives of maize in this region of Santa Catarina belong to the botanical species Zea luxurians.

  10. The identification of sediment sources in a small urban watershed in southern Brazil: an application of sediment fingerprinting.

    PubMed

    Poleto, Cristiano; Merten, Gustavo H; Minella, Jean P

    2009-10-01

    Soil particles eroded from the land surface and transported into rivers by runoff are considered one of the main components of non-point source pollution in urban watersheds. These particles also serve as a vector for a wide variety of both organic and inorganic constituents. As a result, the identification of sediment sources in an urban watershed is necessary not only to understand erosion dynamics, but also to help implement more effective measures to control and/or remediate non-point source pollution. The present study employs sediment 'fingerprinting' to determine the main sediment sources in a small residential urban watershed (0.83 km2) on the outskirts of Porto Alegre in southern Brazil. Based on an evaluation spanning 12 rainfall events, the results show that paved and unpaved roads and the stream channel itself contribute, on average, 46%, 23%, and 31%, respectively, to the suspended sediment flux in the watershed. Furthermore, the source contributions varied both between events and over the course of a single event. This appears to imply that source contributions, at least to some extent, depend on local precipitation patterns. The results from this study indicate that the level of uncertainty in source ascription tends to decline with increasing numbers of tracers; hence, successful sediment fingerprinting and source ascription in complex hydrologic environments, such as urban watersheds, may require the use of a large number of chemical and/or physical tracers.

  11. Identification of and handling of critical irradiance forecast uncertainties using a Random Forest scheme - a case study for southern Brazil

    NASA Astrophysics Data System (ADS)

    Beyer, Hans Georg; Preed Revheim, Pal; Kratenber, Manfred Georg; Zuern, Hans Helmut

    2015-04-01

    For the secure operation of the utility grid, especially in grid with a high penetration of volatile wind or solar generation forecasts of the respective power flows are essential. Full profit of the forecasts can, however not been taken without the knowledge of their uncertainties. Based on irradiance data from southern Brazil we present a scheme for the identification of situations for which elevated forecast errors are to be expected. For this, the classification technique Random Forests applied, using the history of the site specific irradiance data forecast errors together with a set of auxiliary meteorological variables. As byproduct, extracted systematic forecast errors are used to update the forecasts. The predictive performance of the Random Forest models are assessed by the predictions ability to reduce the number of hours or days with forecast errors exceeding the limits, and on the resulting overall forecast RMSE. Limited to none improvements are obtained when predicting next-hour forecast errors, while significant improvements are obtained when predicting next-day forecast errors. Setting a relatively low limit for forecast error exceedance is found to give the largest improvements in terms of reduction of forecast RMSE.

  12. Turbulent statistical characteristics associated to the north wind phenomenon in southern Brazil with application to turbulent diffusion

    NASA Astrophysics Data System (ADS)

    Arbage, Maria Cristina Andres; Degrazia, Gervásio Annes; Welter, Guilherme Sausen; Roberti, Débora Regina; Acevedo, Otávio Costa; de Moraes, Osvaldo Luiz Leal; Ferraz, Simone Teleginski; Timm, Andréa Ucker; Moreira, Virnei Silva

    2008-07-01

    A parameterization for the transport processes in a shear driven planetary boundary layer (PBL) has been established employing turbulent statistical quantities measured during the north wind phenomenon in southern Brazil. Therefore, observed one-dimensional turbulent energy spectra are compared with a spectral model based on the Kolmogorov arguments. The good agreement obtained from this comparison leads to well defined formulations for the turbulent velocity variance, local decorrelation time scale and eddy diffusivity. Furthermore, for vertical regions in which the wind shear forcing is relevant, the eddy diffusivity derived from the north wind data presents a similar profile to those obtained from the non-extensive statistical mechanics theory. Finally, a validation for the present parameterization has been accomplished, using a Lagrangian stochastic dispersion model. The Prairie Grass data set, which presents high mean wind speed, is simulated. The analysis developed in this study shows that the turbulence parameterization constructed from wind data for north wind flow cases is able to describe the diffusion in a high wind speed, shear-dominated PBL.

  13. Water level effect on herbaceous plant assemblages at an artificial reservoir-Lago Azul State Park, Southern Brazil.

    PubMed

    Souza, D C; Ferreira, J D; Bueno, P A A; Iwakura, L; Bueno, R O; Campiolo, J B

    2015-12-01

    This study presents the effect of water level variation on the assemblages of herbaceous species in Mourão I Reservoir, Lago Azul State Park, Southern Brazil. The structure and distribution of populations was examined in February (dry period) and April (rainy period), 2011, in two transects. These transects started at the forest edge towards the center of the lake. The end of the transect coincided with the end of the plants within the lake. On every two meters along of the transects we sampled a wooden square of 0.25 m(2) for species biomass analysis.The macrophyte stand was composed entirely of emergent species. Considering the periods, most species were less frequent in the rainy period (April), but Ipomea ramosissima (Poir.) Choisy, Commelina nudiflora L., Eleocharis acuntagula (Roxb.) Schult. and Verbena litorales (Kunth.) had their frequency increased during this period, probably due to their resistance. The influence of flood as measured by the NMDS point out that both before and after the flood, there are plots with distinct compositions and biomass. The water level variation affects the dynamics of plant composition and structure in marginal areas of the Reservoir.

  14. Leachability of major and minor elements from soils and sediments of an abandoned coal mining area in Southern Brazil.

    PubMed

    Santos, Maria Josefa; Tarley, César Ricardo Teixeira; Cunha, Isabella; Zapelini, Iago; Galunin, Evgeny; Bleinroth, Diego; Vieira, Isadora; Abrão, Taufik

    2015-03-01

    Leachability of major and trace elements from sediment and soil samples of an abandoned coal mining area in southern Brazil was assessed by titration and pH-stat tests according to the SR002.1 and CEN/TS 14429 protocols. Major (Al, Fe, Ca, Mg, and Mn) and trace (Cu, Zn, As, Ni, Pb, Cd, and Hg) elements were quantified in aqueous extracts. Acid and base neutralizing capacity values and pH changes after the addition of certain acid/base amounts were estimated. In general, a decrease in the major metal leaching at pH < 4.0 and an increase at pH > 8.0 was observed. The response to the acid and base additions confirmed that strong acids can cause an effect on Ca- and Mg-bearing silicate phases and Mn oxides, and strong bases can only affect Ca silicates. At pH < 5.0, higher extractability was found for Cu, Zn, Ni, Pb, and Cd. Considering that the samples showed sharp pH changes after acid additions and released major and trace metal into the solution at greater rates, high metal contamination risks can be assumed for the studied area.

  15. Spatial distribution and enteroparasite contamination in peridomiciliar soil and water in the Apucaraninha Indigenous Land, southern Brazil.

    PubMed

    da Silva, Joseane Balan; Piva, Camila; Falavigna-Guilherme, Ana Lúcia; Rossoni, Diogo Francisco; de Ornelas Toledo, Max Jean

    2016-04-01

    The prevalence and distribution of soil and water samples contaminated with enteroparasites of humans and animals with zoonotic potential (EHAZP) in Apucaraninha Indigenous Land (AIL), southern Brazil, was evaluated. An environmental survey was conducted to evaluate the presence of parasitic forms in peridomiciliary soil and associated variables. Soil samples were collected from 40/293 domiciles (10 domiciles per season), from November 2010 to June 2011, and evaluated by modified methods of Faust et al. and Lutz. Analyses of water from seven consumption sites were also performed. The overall prevalence of soil samples contaminated by EHAZP was 23.8 %. The most prevalent parasitic forms were cyst of Entamoeba spp. and eggs of Ascaris spp. The highest prevalence of contaminated soil samples was observed in winter (31 %). The probability map obtained with geostatistical analyses showed an average of 47 % soil contamination at a distance of approximately 140 m. The parasitological analysis of water did not detect Giardia spp. or Cryptosporidium spp. and showed that all collection points were within the standards of the Brazilian law. However, the microbiological analysis showed the presence of Escherichia coli in 6/7 sampled points. Despite the low level of contamination by EHAZP in peridomiciliar soil and the absence of pathogenic protozoa in water, the AIL soil and water (due to the presence of fecal coliforms) are potential sources of infection for the population, indicating the need for improvements in sanitation and water treatment, in addition periodic treatment of the population with antiparasitic.

  16. Poaceae Pollen from Southern Brazil: Distinguishing Grasslands (Campos) from Forests by Analyzing a Diverse Range of Poaceae Species.

    PubMed

    Radaeski, Jefferson N; Bauermann, Soraia G; Pereira, Antonio B

    2016-01-01

    This aim of this study was to distinguish grasslands from forests in southern Brazil by analyzing Poaceae pollen grains. Through light microscopy analysis, we measured the size of the pollen grain, pore, and annulus from 68 species of Rio Grande do Sul. Measurements were recorded of 10 forest species and 58 grassland species, representing all tribes of the Poaceae in Rio Grande do Sul. We measured the polar, equatorial, pore, and annulus diameter. Results of statistical tests showed that arboreous forest species have larger pollen grain sizes than grassland and herbaceous forest species, and in particular there are strongly significant differences between arboreous and grassland species. Discriminant analysis identified three distinct groups representing each vegetation type. Through the pollen measurements we established three pollen types: larger grains (>46 μm), from the Bambuseae pollen type, medium-sized grains (46-22 μm), from herbaceous pollen type, and small grains (<22 μm), from grassland pollen type. The results of our compiled Poaceae pollen dataset may be applied to the fossil pollen of Quaternary sediments.

  17. Surface water quality in the Sinos River basin, in Southern Brazil: tracking microbiological contamination and correlation with physicochemical parameters.

    PubMed

    Dalla Vecchia, Andréia; Rigotto, Caroline; Staggemeier, Rodrigo; Soliman, Mayra Cristina; Gil de Souza, Fernanda; Henzel, Andreia; Santos, Eliane Lemos; do Nascimento, Carlos Augusto; de Quevedo, Daniela Muller; Fleck, Juliane Deise; Heinzelmann, Larissa Schemes; de Matos Almeida, Sabrina Esteves; Spilki, Fernando Rosado

    2015-07-01

    Around the world, enteric viruses are often found in surface waters. This study set out to evaluate the occurrence of adenoviruses (AdVs) in water samples, and its relation to different physical, chemical, and bacteriological parameters [total coliform (TC) and fecal coliform (FC), represented by Escherichia coli]. Monthly samples of 500 ml of raw water were collected from May 2011 to June 2013 in eight abstraction points water treatment stations along three stretches of the Sinos River Basin (SRB), in Southern Brazil and, subsequently, were analyzed using real-time polymerase chain reaction (qPCR). AdVs from different species, from human (HAdV), and from other animals (CAV1-2, BAdV, PAdV, and AvAdV) were detected along the three stretches of the basin, indicating fecal contamination from different sources and proving the inefficiency of the wastewater treatment in the waters of the SRB and intensifying the strong influence of human activities that can contribute to the presence of inhibitory substances such as organic acids in surface of these waters. Statistical analyses revealed no significant correlations between the concentrations of TC and FC and the concentrations of AdVs. We observed a small, nonconstant, and unstable correlation between viruses and physicochemical parameters. These correlations were not sufficiently consistent to establish a reliable association; therefore, this study corroborates that only the viral assay itself is reliable for the diagnosis of fecal contamination by viruses in environmental samples.

  18. [Exploring multiple trajectories of causality: collaboration between Anthropology and Epidemiology in the 1982 birth cohort, Pelotas, Southern Brazil].

    PubMed

    Béhague, Dominique P; Gonçalves, Helen

    2008-12-01

    Although the relationship between epidemiology and anthropology has a long history, it has generally been comprised of the integration of quantitative and qualitative methods. Only recently have the two fields begun to converge along theoretical lines, leading to a growing mutual interest in explaining rather than simply describing phenomena. This paper aimed to illustrate how ethnographic analyses can be used to assist with the in-depth and theoretically-imbued interpretation of epidemiological results. The anthropological analysis presented in this paper used ethnographic data collected as part of the ongoing 1982 birth cohort study, between 1997 and 2007 in Pelotas, Southern Brazil. Analyses were framed according to the results presented in two of the epidemiological articles published in this series on the determinants of mental morbidity and age of sexual initiation. The ethnographic results show that statistical associations consist of multiple pathways of influence and causality that generally correspond to the unique experiences of specific subgroups. In exploring these pathways, the paper highlights the importance of an additional set of mediating factors that account for epidemiological results; these include the awareness and experience of inequities, the role of violence in everyday life, traumatic life events, increasing social isolation and emotional introversion as a response to life's difficulties, and differing approaches towards socio-psychological maturation. Theoretical and methodological collaboration between anthropology and epidemiology is important for public health, as it has positively modified both fields.

  19. Medicinal plants used by 'Passo da Ilha' rural community in the city of Pato Branco, southern Brazil.

    PubMed

    Marchese, José A; Ming, Lin C; Franceschi, Lucia de; Camochena, Rubia C; Gomes, Greice D R; Paladini, Marcos V; Capelin, Diogo; Marchese, Cristine F

    2009-12-01

    The scope of this work was to rescue and document the traditional knowledge regarding the medicinal plants used by Passo da Ilha rural community, in Pato Branco, Paraná State, Southern Brazil (26 degrees 11' S, 52 degrees 36' W and 760 m high). Structured interviews were made in field research with 16 informants who had the traditional knowledge about medicinal plants. The research was carried out from October to December 2000. The plants were collected in the field, identified and their vouchers were housed at the Herbarium 'Irina Delanova De Gemtchjnicov' (BOTU) of São Paulo State University, in Botucatu. A total of 47 botanical families and 114 species of medicinal plants were identified. These plants ere suitable for ore than 30 different edicinal uses. The residents are mainly of European descent, which justify the presence of many exotic plants. The knowledge on how to use medicinal plants properly is held mainly by the females, and decreases in the newer generations, denoting 'cultural erosion' in progress.

  20. Maternal Gene Polymorphisms Involved in Folate Metabolism as Risk Factors for Down Syndrome Offspring in Southern Brazil

    PubMed Central

    Brandalize, Ana Paula Carneiro; Bandinelli, Eliane; Santos, Pollyanna Almeida Dos; Schüler-Faccini, Lavínia

    2010-01-01

    This study aimed to investigate the role of maternal polymorphisms, as well as their risk genotypes combinations of MTR A2756G, MTRR A66G, CBS 844ins68, and RFC A80G, involved in folate/homocysteine metabolism, as possible risk factors for Down syndrome (DS) in Southern Brazil. A case-control study was conducted with 239~mothers of DS children and 197 control mothers. The investigation of polymorphisms was performed by PCR and PCR-RFLP. The distribution of genotypic variants was similar in both groups when they were analyzed separately. An investigation of combined risk genotypes showed that the risk of having a DS child for one, two or three risk genotypes was 6.23, 6.96 and 5.84 (95%CI 1.48–26.26; 1.69–28.66; 1.37–24.86), respectively. The combined MTRR 66G and MTHFR 677T alleles were significantly more common among mothers of children with DS than among control mothers (OR 1.55; IC 95% 1.03–2.35). The results show that individual polymorphisms studied in this work are not associated with DS; however, the effects of the combined risk genotypes among MTR, MTRR, CBS and RFC genes are considered maternal risk factors for DS offspring in our population. PMID:21045269

  1. Epidemiological findings and laboratory evaluation of sporotrichosis: a description of 103 cases in cats and dogs in southern Brazil.

    PubMed

    Madrid, Isabel Martins; Mattei, Antonella Souza; Fernandes, Cristina Gevehr; Nobre, Márcia de Oliveira; Meireles, Mário Carlos Araújo

    2012-04-01

    Sporotrichosis is a subcutaneous mycosis, which affects mainly small animals, and is considered an important public health disease. This paper describes the epidemiological and laboratory characteristics of 103 clinical cases of sporotrichosis diagnosed over a 10-year period in southern Brazil. The 92 cats and 11 dogs from eight municipalities in Rio Grande do Sul State developed especially the disseminated cutaneous and fixed cutaneous forms of the disease. Respiratory signs such as sneezing, serous nasal discharge and dyspnea were found in about 57% of the animals. The detection of Sporothrix schenckii in different clinical samples showed highest isolation in testicles (46.6%), oral cavity (45.2%) and conjunctival mucosa (38.1%). A differentiated histological pattern was found between the fixed cutaneous and disseminated cutaneous (DC) manifestations of the disease; well-organized granulomas of nodular distribution and various fungal structures prevailed in the DC form in cats. Melanin detection in S. schenckii cells by the Fontana-Masson technique was positive in 45.4% of the samples. The study revealed that the State of Rio Grande do Sul is an endemic sporotrichosis area and demonstrated the possibility of involvement of other pathways in the infection and spread of the disease. In addition, it emphasized the importance of laboratory tests for mycosis confirmation, especially in dogs that develop clinical manifestations without the presence of cutaneous lesions.

  2. Population prevalence of hereditary breast cancer phenotypes and implementation of a genetic cancer risk assessment program in southern Brazil

    PubMed Central

    2009-01-01

    In 2004, a population-based cohort (the Núcleo Mama Porto Alegre - NMPOA Cohort) was started in Porto Alegre, southern Brazil and within that cohort, a hereditary breast cancer study was initiated, aiming to determine the prevalence of hereditary breast cancer phenotypes and evaluate acceptance of a genetic cancer risk assessment (GCRA) program. Women from that cohort who reported a positive family history of cancer were referred to GCRA. Of the 9218 women enrolled, 1286 (13.9%) reported a family history of cancer. Of the 902 women who attended GCRA, 55 (8%) had an estimated lifetime risk of breast cancer ≥ 20% and 214 (23.7%) had pedigrees suggestive of a breast cancer predisposition syndrome; an unexpectedly high number of these fulfilled criteria for Li-Fraumeni-like syndrome (122 families, 66.7%). The overall prevalence of a hereditary breast cancer phenotype was 6.2% (95%CI: 5.67-6.65). These findings identified a problem of significant magnitude in the region and indicate that genetic cancer risk evaluation should be undertaken in a considerable proportion of the women from this community. The large proportion of women who attended GCRA (72.3%) indicates that the program was well-accepted by the community, regardless of the potential cultural, economic and social barriers. PMID:21637504

  3. Community of arthropod ectoparasites of two species of Turdus Linnaeus, 1758 (Passeriformes: Turdidae) in southern Rio Grande do Sul, Brazil.

    PubMed

    da Cunha Amaral, Hugo Leonardo; Bergmann, Fabiane Borba; dos Santos, Paulo Roberto Silveira; Krüger, Rodrigo Ferreira; Graciolli, Gustavo

    2013-02-01

    This study was aimed at describing the community of arthropod ectoparasites associated with sympatric populations of Turdus amaurochalinus and Turdus rufiventris and analyzing the aggregation patterns of the chewing lice species, during reproductive and nonreproductive periods, of both Turdus species in three areas of the Atlantic forest in southern Rio Grande do Sul state (RS), Brazil. Altogether, we captured 36 specimens of T. amaurochalinus and 53 specimens of T. rufiventris. We identified two families of chewing lice, Menoponidae and Philopteridae, with Myrsidea and Brueelia as the most prevalent and abundant on both host birds. The lowest aggregation levels of chewing lice Myrsidea and Brueelia occurred during the reproductive period of both host species, suggesting a reproductive synchronization and a dispersion period. The most prevalent feather mite on T. amaurochalinus was Proctophyllodes weigoldi, and on T. rufiventris, Trouessartia serrana. Analges sp. and Pteronyssoides sp. were not observed on T. rufiventris. We identified three species of ticks; Ixodes auritulus was the most prevalent and abundant on the birds. Ornithoica vicina was the only hippoboscid fly collected, and only on T. amaurochalinus. The richness of ectoparasites was greater on T. amaurochalinus than on T. rufiventris. For T. amaurochalinus, the mean richness was lesser in winter compared to spring and autumn; however, we observed no variation in the mean richness of ectoparasites for T. rufiventris during the same seasons.

  4. Serosurvey of Borrelia in dogs, horses, and humans exposed to ticks in a rural settlement of southern Brazil.

    PubMed

    Nascimento, Denise Amaral Gomes; Vieira, Rafael Felipe da Costa; Vieira, Thállitha Samih Wischral Jayme; Toledo, Roberta Dos Santos; Tamekuni, Katia; Santos, Nelson Jessé Rodrigues Dos; Gonçalves, Daniela Dibb; Vieira, Maria Luísa; Biondo, Alexander Welker; Vidotto, Odilon

    2016-01-01

    The aims of the present study were to serosurvey dogs, horses, and humans highly exposed to tick bites for anti-Borrelia burgdorferi s.l. antibodies, identify tick species present, and determine risk factors associated with seropositivity in a rural settlement of Paraná State, southern Brazil. Eighty-seven residents were sampled, along with their 83 dogs and 18 horses, and individual questionnaires were administered. Immunofluorescence antibody test (IFAT) was performed on serum samples and positive samples were subjected to western blot (WB) analysis. Anti-B. burgdorferi antibodies were found in 4/87 (4.6%) humans, 26/83 (31.3%) dogs, and 7/18 (38.9%) horses by IFAT, with 4/4 humans also positive by WB. Ticks identified were mostly from dogs and included 45/67 Rhipicephalus sanguineus, 21/67 Amblyomma ovale, and 1/67 A. cajennense sensu lato. All (34/34) horse ticks were identified as A. cajennense s.l.. No significant association was found when age, gender, or presence of ticks was correlated to seropositivity to Borrelia sp. In conclusion, although anti-Borrelia antibodies have been found in dogs, horses and their owners from the rural settlement, the lack of isolation, molecular characterization, absence of competent vectors and the low specificity of the commercial WB kit used herein may have impaired risk factor analysis.

  5. Presence of Zea luxurians (Durieu and Ascherson) Bird in Southern Brazil: Implications for the Conservation of Wild Relatives of Maize

    PubMed Central

    2015-01-01

    Records of the occurrence of wild relatives of maize in South American lowlands are unprecedented, especially in sympatric coexistence with landraces. This fact is relevant, because regions of occurrence of wild relatives of cultivated plants should be a priority for conservation, even if they do not correspond to the center of origin of the species. The aim of this study was to identify and characterize the wild relatives of maize in the Far West of Santa Catarina, southern Brazil. Therefore, phenotypic characterization was performed for five populations, based on 22 morphological traits deemed as fundamental for classifying the species of the genus Zea, and validated through the characterization of chromosomal knobs of two populations. The occurrence and distribution of teosinte populations were described through semi-structured interviews applied to a sample of 305 farmers. A total of 136 teosinte populations were identified; 75% of them occur spontaneously, 17% are cultivated populations, and 8% occur both ways, for the same farm. Populations that were characterized morphologically had trapezoidal fruits mostly, upright tassel branch (4–18), non-prominent main branch and glabrous glumes, with two protruding outer ribs and 8 inner ribs, on average. Cytogenetic analysis identified 10 pairs of homologous chromosomes (2n = 20) with 26 knobs, located in the terminal region of all chromosomes. The similarity of these results with the information reported in the literature indicates that the five populations of wild relatives of maize in this region of Santa Catarina belong to the botanical species Zea luxurians. PMID:26488577

  6. Poaceae Pollen from Southern Brazil: Distinguishing Grasslands (Campos) from Forests by Analyzing a Diverse Range of Poaceae Species

    PubMed Central

    Radaeski, Jefferson N.; Bauermann, Soraia G.; Pereira, Antonio B.

    2016-01-01

    This aim of this study was to distinguish grasslands from forests in southern Brazil by analyzing Poaceae pollen grains. Through light microscopy analysis, we measured the size of the pollen grain, pore, and annulus from 68 species of Rio Grande do Sul. Measurements were recorded of 10 forest species and 58 grassland species, representing all tribes of the Poaceae in Rio Grande do Sul. We measured the polar, equatorial, pore, and annulus diameter. Results of statistical tests showed that arboreous forest species have larger pollen grain sizes than grassland and herbaceous forest species, and in particular there are strongly significant differences between arboreous and grassland species. Discriminant analysis identified three distinct groups representing each vegetation type. Through the pollen measurements we established three pollen types: larger grains (>46 μm), from the Bambuseae pollen type, medium-sized grains (46–22 μm), from herbaceous pollen type, and small grains (<22 μm), from grassland pollen type. The results of our compiled Poaceae pollen dataset may be applied to the fossil pollen of Quaternary sediments. PMID:27999585

  7. Species richness of fern and lycophyte in an urban park in the Rio dos Sinos basin, Southern Brazil.

    PubMed

    Schmitt, J L; Goetz, M N B

    2010-12-01

    In the northeastern region of the State of Rio Grande do Sul in Southern Brazil, specifically in the Rio dos Sinos basin, urban parks are an important alternative for the conservation of the remaining natural habitats, as well as for the maintenance and perpetuation of biodiversity. A floristic survey of fern and lycophyte species in the Parque Municipal Henrique Luís Roessler (PMHLR) (29º 41' S and 51º 06' W; alt. 16.4 m) in Novo Hamburgo, (State of Rio Grande do Sul, RS) described their life-forms, as well as substrates and preferential environments. Forty-three species, 30 genera and 15 families were found, 39 of which were ferns. The hemicryptophytes had the highest species richness (26), 17 of which were repent, and nine, rosulate. Most species were found in terrestrial substrates (32) and inside the forest (29). Species richness in the PMHLR corresponded to about 13% of the total fern and lycophyte species listed for the State of Rio Grande do Sul, which demonstrates the importance of conservation areas in the Rio dos Sinos basin as an alternative to the preservation of local biodiversity.

  8. Amblyomma aureolatum and Ixodes auritulus (Acari: Ixodidae) on birds in southern Brazil, with notes on their ecology.

    PubMed

    Arzua, Márcia; Navarro Da Silva, Mário Antonio; Famadas, Kátia Maria; Beati, Lorenza; Barros-Battesti, Darci Moraes

    2003-01-01

    Between January 1999 and December 2000, 876 bird specimens were captured in three different ecological environments from the Reinhard Maack Park, Curitiba, State of Paraná, southern Brazil. A total of 142 birds (16.2%) were infested with Amblyomma aureolatum (Pallas 1772) (N=699) and/or Ixodes auritulus Neumann, 1904 (N=18) ticks. Questing A. aureolatum nymphs (N=2) and adults (N=5) were also collected from the soil and the vegetation. None of the I. auritulus were collected off-host. We collected only immatures of A. aureolatum on birds, but all life stages of I. auritulus. The latter species was collected on Turdus rufiventris and on Synallaxis ruficapilla, which is herein recognized as a host of I. auritulus for the first time. Moreover, this is also the first report of A. aureolatum infesting birds, and 16 different bird species were found infested. It was observed that larval infestation was positively correlated with the dry and cold season, while nymphal infestation was positively correlated with the warm and rainy season. Although only 2-years worth of data is provided, our results suggest the infestation of birds by ticks was significantly higher at the biotopes formed by forest at its first stage of regeneration 'capoeira' and the original Araucaria forest habitat 'mata' than the ecotone between forest and urban areas 'peripheral area'.

  9. Exploring multiple trajectories of causality: collaboration between Anthropology and Epidemiology in the 1982 birth cohort, Pelotas, Southern Brazil

    PubMed Central

    Béhague, Dominique P; Gonçalves, Helen

    2009-01-01

    OBJECTIVE: Although the relationship between epidemiology and anthropology has a long history, it has generally been comprised of the integration of quantitative and qualitative methods. Only recently have the two fields begun to converge along theoretical lines, leading to a growing mutual interest in explaining rather than simply describing phenomena. This paper aimed to illustrate how ethnographic analyses can be used to assist with the in-depth and theoretically-imbued interpretation of epidemiological results. METHODS: The anthropological analysis presented in this paper used ethnographic data collected as part of the ongoing 1982 birth cohort study, between 1997 and 2007 in Pelotas, Southern Brazil. Analyses were framed according to the results presented in two of the epidemiological articles published in this series on the determinants of mental morbidity and age of sexual initiation. RESULTS AND CONCLUSIONS: The ethnographic results show that statistical associations consist of multiple pathways of influence and causality that generally correspond to the unique experiences of specific subgroups. In exploring these pathways, the paper highlights the importance of an additional set of mediating factors that account for epidemiological results; these include the awareness and experience of inequities, the role of violence in everyday life, traumatic life events, increasing social isolation and emotional introversion as a response to life's difficulties, and differing approaches towards socio-psychological maturation. Theoretical and methodological collaboration between anthropology and epidemiology is important for public health, as it has positively modified both fields. PMID:19142353

  10. A rapid fish radiation associated with the last sea-level changes in southern Brazil: the silverside Odontesthes perugiae complex.

    PubMed Central

    Beheregaray, Luciano B; Sunnucks, Paul; Briscoe, David A

    2002-01-01

    Coastal freshwater fishes provide valuable models for studying the role of the last glaciations in promoting speciation. To date, the great majority of studies are of Northern Hemisphere taxa, and reflect the influence of vicariant events during, or prior to, the Pleistocene. Microsatellite markers and mitochondrial DNA sequences were used to investigate patterns of population divergence and evolutionary relationships in a freshwater group of silverside fishes (Odontesthes perugiae complex), endemic to the recently formed coastal plain of southern Brazil. Lacustrine morphotypes showed concordant patterns of genetic and morphological divergence consistent with the geographical history of the coastal plain. The results support the proposal of a silverside radiation chronologically shaped by the sea-level changes of the Pleistocene and Holocene. The radiating lineage comprises a minimum of three allopatric and two sympatric lacustrine species. Four species displayed extremely high levels of genetic variation and some of the most rapid speciation rates reported in fishes. These features were related to a marine-estuarine origin of the radiation. To the best of our knowledge, this study represents the first molecular phylogeographic survey of a coastal radiation in South America. PMID:11788038

  11. Dental pain, use of dental services and oral health-related quality of life in southern Brazil.

    PubMed

    Cavalheiro, Charles Henrique; Abegg, Claides; Fontanive, Victor Nascimento; Davoglio, Rosane Silvia

    2016-08-18

    This study aimed at assessing the relationship between dental pain and the reason for using dental services and oral health quality of life in people aged 50 to 74 years in southern Brazil. A cross-sectional population-based study was conducted with 720 individuals aged 50 to 74 years, living in three health districts in the city of Porto Alegre. Dental impacts on daily life and sociodemographic data were assessed using structured interviews. The Oral Impacts on Daily Performance - OIDP instrument was used to measure oral impacts. The information was analyzed by Poisson regression with robust variance adjustment, taking into account cluster sampling. Dental pain was present in 32.5% of those reporting an oral impact on their daily activities. Dental pain most frequently affected talking (37.6%), cleaning teeth and gums (37.0%) and enjoying the companionship of people (36.5%). After adjustments to the multivariate analysis, the reason for dental visits due to dental pain was found to have a high impact on daily activities [RP 1.68 (1.11 - 2.54].

  12. Assessing the spatial pattern of a river water quality in southern Brazil by multivariate analysis of biological and chemical indicators.

    PubMed

    Cassanego, M B B; Droste, A

    2017-03-01

    This study assessed the genotoxicity and chemical quality of the Rio dos Sinos, southern Brazil. During two years, bimonthly, cuttings of Tradescantia pallida var. purpurea with flower buds were exposed to river water samples from Caraá, Santo Antônio da Patrulha, Taquara and Campo Bom, which are municipalities located in the upper, middle and lower stretches of the Rio dos Sinos basin. Simultaneously, chemical parameters were analyzed, rainfall data were surveyed and negative (distilled water) and positive (0.1% formaldehyde) controls were made. Micronuclei (MCN) frequencies were determined in tetrads of pollen grain mother cells. From the upper stretch toward the lower, there was an increase in the frequency of MCN and in concentrations of chemical parameters. Cadmium, lead, copper, total chromium and zinc were present at the four sites and a concentration gradient was not demonstrated along the river. The multivariate analysis revealed that two principal components exist, which accounted for 62.3% of the observed variances. Although genotoxicity was observed in Santo Antônio da Patrulha, the water presented higher mean values for most of the assessed parameters, in the lower stretch, where urbanization and industrialization are greater. The spatial and temporal pattern of water quality observed reinforces the importance of considering the environmental factors and their effects on organisms in an integrated way in watercourse monitoring programs.

  13. Infection of humans and animals with Echinococcus granulosus (G1 and G3 strains) and E. ortleppi in Southern Brazil.

    PubMed

    de la Rue, Mario L; Takano, Keishi; Brochado, Joaquim F; Costa, Carmem V; Soares, Antonio G; Yamano, Kimiaki; Yagi, Kinpei; Katoh, Yoshinobu; Takahashi, Kenichi

    2011-04-19

    The Rio Grande do Sul state, in Southern Brazil, is one of the foci of human cystic echinococcosis (CE). The sheep strain (G1) of Echinococcus granulosus and Echinococcus ortleppi (also known as cattle strain G5) have been reported before to infect livestock. However, up to the present, no molecular data are available on isolates of the E. granulosus complex from humans and dogs. The present study analyzed hydatid cysts from 6 CE patients and adult worms from 12 dogs. Sequencing of the mitochondrial cox1 and 12S rRNA genes detected the E. granulosus G1 genotype from four human cases, the G3 genotype (or buffalo strain) from one human case and E. ortleppi from another human case, respectively. Ten of the twelve dogs were found infected with the G1 genotype, and one dog each harbored worms of the G3 genotype and E. ortleppi. Obvious morphological differences were recognized between the G1 and E. ortleppi adult worms from dogs in this region. The buffalo strain (G3) is for the first time reported from South America.

  14. Water quality parameters, biomarkers and metal bioaccumulation in native fish captured in the Ilha River, southern Brazil.

    PubMed

    Dalzochio, Thaís; Ressel Simões, Leonardo Airton; Santos de Souza, Mateus; Prado Rodrigues, Gabriela Zimmermann; Petry, Ismael Evandro; Andriguetti, Natália Bordin; Herbert Silva, Gláucia Joselaine; Gehlen, Günther; Basso da Silva, Luciano

    2017-09-19

    The Ilha River is one of the main tributaries of the Sinos River, southern Brazil, and it is located in an area characterized by low population density and presence of agricultural activities. Thus, this study aimed to assess the water quality of two sites of the Ilha River (source and mouth, S1 and S2 respectively) in five sampling periods using water physicochemical and microbiological analyses, biomarkers, such as condition factor, micronucleus test, gill histopathological analysis, and metal bioaccumulation in the native fish Bryconamericus iheringii. Mean values of BOD5, thermotolerant coliforms, aluminum, iron and lead exceeded the limits established by the Brazilian legislation for surface waters at both sampling sites. Significant higher micronucleus, nuclear abnormalities and mucous cells frequencies were found at S2 in, at least, one sampling period, whereas fish from S1 presented significant lower condition factor, higher frequencies of lamellar alterations and higher concentrations of chromium and nickel in muscle. Additionally, concentrations of cadmium, chromium and lead in fish muscle exceeded the limits considered safe for human consumption at both sites in at least one sampling period. Data from our study evidenced the mouth of the Ilha River suffers from point genotoxic effects, whereas the source is also contaminated by metals, despite being located in an area under minor anthropic activities. Copyright © 2017 Elsevier Ltd. All rights reserved.

  15. DETECTION OF Leishmania (Viannia) IN Nyssomyia neivai AND Nyssomyia whitmani BY MULTIPLEX POLYMERASE CHAIN REACTION, IN SOUTHERN BRAZIL

    PubMed Central

    Neitzke-Abreu, Herintha Coeto; Reinhold-Castro, Kárin Rosi; Venazzi, Mateus Sabaini; Scodro, Regiane Bertin de Lima; Dias, Alessandra de Cassia; Silveira, Thaís Gomes Verzignassi; Teodoro, Ueslei; Lonardoni, Maria Valdrinez Campana

    2014-01-01

    Sandflies transmit pathogens of leishmaniasis. The natural infection of sandflies by Leishmania (Viannia) was assessed in municipalities, in the state of Paraná, in Southern Brazil. Sandflies were collected with Falcão and Shannon traps. After dissection in search of flagellates in digestive tubes and identification of the species, female sandflies were submitted to the Multiplex Polymerase Chain Reaction (multiplex PCR) for detection of the fragment of the kDNA of Leishmania (Viannia) and the fragment from the IVS6 cacophony gene region of the phlebotomine insects. The analysis was performed in pools containing seven to 12 guts from females of the same species. A total of 510 female sandflies were analyzed, including nine Migonemyia migonei, 17 Pintomyia fischeri, 216 Nyssomyia neivai, and 268 Nyssomyia whitmani. Although none of the females was found naturally infected by flagellates through dissection, the fragment of DNA from Leishmania (Viannia) was shown by multiplex PCR in one sample of Ny. neivai (0.46%) and three samples of Ny. whitmani (1.12%). It was concluded that Ny. neivai and Ny. whitmani are susceptible to Leishmania infection, and that multiplex PCR can be used in epidemiological studies to detect the natural infection of the sandfly vector, because of its sensitivity, specificity and feasibility. PMID:25229218

  16. Wilson's disease in Southern Brazil: genotype-phenotype correlation and description of two novel mutations in ATP7B gene.

    PubMed

    Bem, Ricardo Schmitt de; Raskin, Salmo; Muzzillo, Dominique Araújo; Deguti, Marta Mitiko; Cançado, Eduardo Luiz Rachid; Araújo, Thiago Ferreira; Nakhle, Maria Cristina; Barbosa, Egberto Reis; Munhoz, Renato Puppi; Teive, Hélio Afonso Ghizoni

    2013-08-01

    Wilson's disease (WD) is an inborn error of metabolism caused by abnormalities of the copper-transporting protein encoding gene ATP7B. In this study, we examined ATP7B for mutations in a group of patients living in southern Brazil. 36 WD subjects were studied and classified according to their clinical and epidemiological data. In 23 subjects the ATP7B gene was analyzed. Fourteen distinct mutations were detected in at least one of the alleles. The c.3207C>A substitution at exon 14 was the most common mutation (allelic frequency=37.1%) followed by the c.3402delC at exon 15 (allelic frequency=11.4%). The mutations c.2018-2030del13 at exon 7 and c.4093InsT at exon 20 are being reported for the first time. The c.3207C>A substitution at exon 14, was the most common mutation, with an allelic frequency of 37.1%. This mutation is the most common mutation described in Europe.

  17. Angiotensin I-converting enzyme gene polymorphism in two ethnic groups living in Brazil's southern region: association with age.

    PubMed

    Da Cruz, Ivana Beatrice Mânica; Oliveira, Graziela; Taufer, Maristela; Leal, Ney Furhmann; Schwanke, Carla Helena; Glock, Luiz; Moriguchi, Yukio; Moriguchi, Emilio Hideyuki

    2003-09-01

    Several studies have been published on the association between ACE (angiotensin I-converting enzyme) polymorphism and longevity. However, the results are controversial. We analyzed the association between ACE polymorphism and age in two different ethnic populations: a population originating from different European countries (Gaucha Population, GP) and a population originating from Japan (JP). Both populations live in Brazil's southern region. We determined the ACE genotype in 834 persons aged 10-104 years. The ACE genotype and allele frequencies differed between the two populations, with the D allele being more frequent in GP than JP. We found an association between the DD genotype and D allele and age in the GP group only. The ACE polymorphism-age association occurred at age >60 years in the GP population with decreasing II frequency. We cannot dismiss the possibility of the association between ACE polymorphism and age involving linkage disequilibrium, since the nature of this phenomenon is still controversial. From our studies, it appears that there is a correlation between age, ethnicity, and ACE polymorphism. More of such studies are warranted, as further investigation in this area will have a high clinical relevance to cardiovascular disease and aging research.

  18. Metabolic Profiling and Classification of Propolis Samples from Southern Brazil: An NMR-Based Platform Coupled with Machine Learning.

    PubMed

    Maraschin, Marcelo; Somensi-Zeggio, Amélia; Oliveira, Simone K; Kuhnen, Shirley; Tomazzoli, Maíra M; Raguzzoni, Josiane C; Zeri, Ana C M; Carreira, Rafael; Correia, Sara; Costa, Christopher; Rocha, Miguel

    2016-01-22

    The chemical composition of propolis is affected by environmental factors and harvest season, making it difficult to standardize its extracts for medicinal usage. By detecting a typical chemical profile associated with propolis from a specific production region or season, certain types of propolis may be used to obtain a specific pharmacological activity. In this study, propolis from three agroecological regions (plain, plateau, and highlands) from southern Brazil, collected over the four seasons of 2010, were investigated through a novel NMR-based metabolomics data analysis workflow. Chemometrics and machine learning algorithms (PLS-DA and RF), including methods to estimate variable importance in classification, were used in this study. The machine learning and feature selection methods permitted construction of models for propolis sample classification with high accuracy (>75%, reaching ∼90% in the best case), better discriminating samples regarding their collection seasons comparatively to the harvest regions. PLS-DA and RF allowed the identification of biomarkers for sample discrimination, expanding the set of discriminating features and adding relevant information for the identification of the class-determining metabolites. The NMR-based metabolomics analytical platform, coupled to bioinformatic tools, allowed characterization and classification of Brazilian propolis samples regarding the metabolite signature of important compounds, i.e., chemical fingerprint, harvest seasons, and production regions.

  19. Effects of a fire on a population of treefrogs (Scinax cf. alter, Lutz) in a restinga habitat in southern Brazil.

    PubMed

    Rocha, C F D; Ariani, C V; Menezes, V A; Vrcibradic, D

    2008-08-01

    The area of the Dunas da Joaquina, in Santa Catarina island, contains one of the most important remnants of restinga habitat in Santa Catarina State, southern Brazil. In December 2003, a fire occurred in a portion of this area, affecting most of the vegetation, including the bromeliad community. In this study, the density of individuals and the diet composition of the bromelicolous treefrog Scinax cf. alter were compared between the area affected by the fire and an adjacent unburned area. One-hundred-and-fifty-eight ground bromeliads (Vriesea friburguensis) were dissected and searched for the presence of treefrogs among their leaves. We found 30 frogs in 29.5% (23/78) of the bromeliads from the unburned site, with a mean of 1.3 frogs per rosette, and 15 frogs in 12.5% (10/80) of the bromeliads from the burned site, with a mean of 1.6 frogs per rosette. Eight (27%) of the frogs from the unburned site and eleven (73%) of those from the burned site had empty stomachs. Frogs from the burned site also contained less prey per stomach than those from the unburned site. The data suggest that the fire has negatively affected the local population of Scinax cf. alter, though it is possible that the population can recover.

  20. [Adhering to or resisting tobacco cultivation? Stories of rural workers from a producing region in southern Brazil].

    PubMed

    Riquinho, Deise Lisboa; Hennington, Elida Azevedo

    2014-10-01

    This study sought to understand the standpoints of farming families who have resisted or abandoned tobacco cultivation and also the perceptions of State representatives, civil society organizations and the tobacco industry regarding the implementation and the continuity of this cultivation in a producing region in southern Brazil. In this ethnographic study, 27 semi-structured interviews were conducted with farming families and key informants. Besides that, participant observation was conducted. For those who abandoned tobacco cultivation, the main reason mentioned was indebtedness, and for those who have never planted, the main reasons alleged were fear of indebtedness, a limited workforce or religious issues. State representatives and civil society organizations highlighted illusory financial returns as the main reason for tobacco cultivation. Tobacco industry representatives insisted on the difficulty of commercializing the production and the low price of foodstuffs as a stimulus to adhere to the integrated system. It is therefore concluded that the formation of associations and the development of crop rotation plans are important strategies to be adopted to facilitate the change of cultivation in order to promote better working and living conditions for the rural population.

  1. Efficacy of entecavir and tenofovir in chronic hepatitis B under treatment in the public health system in southern Brazil

    PubMed Central

    Pereira, Camila V; Tovo, Cristiane Valle; Grossmann, Thiago K; Mirenda, Henrique; Dal-Pupo, Bruna B; de Almeida, Paulo RL; de Mattos, Angelo A

    2016-01-01

    There are about 350 million hepatitis B virus (HBV) carriers worldwide and chronic HBV is considered a major public health problem. The objective of the present study was to assess the effectiveness of the nucleos(t)ide analogues tenofovir (TDF) and entecavir (ETV) in the treatment of chronic HBV. A cross-sectional study was carried out from March-December 2013, including all patients with chronic HBV, over 18 years of age, undergoing therapy through the public health system in southern Brazil. Only the data relating to the first treatments performed with TDF or ETV were considered. Retreatment, co-infection, transplanted or immunosuppressed patients were excluded. Six hundred and forty patients were evaluated, of which 336 (52.5%) received TDF and 165 (25.8%) ETV. The other 139 (21.7%) used various combinations of nucleos(t)ide analogues and were excluded. The negativation of viral load was observed in 87.3% and 78.8% and the negativation of hepatitis B e antigen was achieved in 79% and 72% of those treated with ETV or TDF, respectively. Negativation of hepatitis B surface antigen was not observed. There was no occurrence of adverse effects. This is a real-life study demonstrating that long-term treatment with ETV and TDF is both safe and effective. PMID:27074254

  2. Inferring sex and caste seasonality patterns in three species of bumblebees from southern Brazil using biological collections.

    PubMed

    de Paula, G A R; Melo, G A R

    2015-02-01

    Biological collections may often be an important source of information about natural history, behavioral habits, and ecology as they contain samples of organisms collected in different places and moments in times. In here, we used museum specimens to examine how populations of three species of Bombus-Bombus bellicosus Smith, Bombus morio (Swederus), and Bombus pauloensis Friese-vary over time with respect to abundance and biomass of each sex and caste. The study included all specimens of these three bumblebee species deposited in the insect collection of the Federal University of Parana that were collected in eastern Paraná, in southern Brazil. Seasonality is most noticeable in the peak of queen activity (number of individuals in collections) in early spring, after dormancy during the winter. Queens then founded nests, and workers and males began to become more abundant during spring and summer. Worker abundance peaked at the end of summer and beginning of fall and may correspond to increasing activity for production of new males and gynes. Male abundance peaks are during the reproductive period. Thus, we show with these data and analyses that museum collections can be very useful tools to examine temporal and ecological processes. We recommend greater use of museum accessions to explore these kinds of patterns that may otherwise go unnoticed.

  3. Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil

    PubMed Central

    da Costa, Mateus Matiuzzi; Drescher, Guilherme; Maboni, Franciele; Weber, Shana; de Avila Botton, Sônia; Vainstein, Marilene Henning; Schrank, Irene Silveira; de Vargas, Agueda Castagna

    2008-01-01

    The present study determined the molecular and resistance patterns of E. coli isolates from urinary tract of swine in Southern of Brazil. Molecular characterization of urinary vesicle samples was performed by PCR detection of virulence factors from ETEC, STEC and UPEC. From a total of 82 E. coli isolates, 34 (38.63%) harbored one or more virulence factors. The frequency of virulence factors genes detected by PCR were: pap (10.97%), hlyA (10.97%), iha (9.75%), lt (8.53%), sta (7.31%) sfa (6.09%), f4 (4.87%), f5 (4.87%), stb (4.87%), f6 (1.21%) and f41 (1.21%). Isolates were resistant to penicillin (95.12%), lincomycin (93.9%), erythromycin (92.68%), tetracycline (90.24%), amoxicillin (82.92%), ampicillin (74.39%), josamycin (79.26%), norfloxacin (58.53%), enrofloxacin (57.31%), gentamicin (39.02%), neomycin (37.8%), apramycin (30.48%), colistine (30.48%) and cefalexin (6.09%). A number of 32 (39.02%) E. coli isolates harbored plasmids. PMID:24031300

  4. Two new species of the Brachycephalus pernix group (Anura: Brachycephalidae) from the state of Paraná, southern Brazil

    PubMed Central

    Blackburn, David C.; Stanley, Edward L.; Pie, Marcio R.; Bornschein, Marcos R.

    2017-01-01

    We describe two new species of miniaturized toadlet in the B. pernix group of Brachycephalus (Anura: Brachycephalidae) from the Atlantic Forest of the state of Paraná, southern Brazil. The first new species is distinguished from all congeners by the pale red coloration from the head to the pelvic region, with sides of the body and thighs dorsally yellowish green. It is known only from the type locality in a cloud forest at altitudes ranging between 1,144–1,228 m a.s.l. The second species, although more closely related to B. izecksohni, is morphologically similar to B. brunneus in its overall brown coloration, but distinct from that species in the color of the iris (black with conspicuous golden spots, instead of entirely black). It was found on three mountains, at altitudes between 1,095–1,320 m a.s.l., and in vegetation types including cloud forest, montane forest, and secondary forest. The two new species exhibit neither vertebral fusions nor osteoderms, but one has both a distinct neopalatine and well-developed odontoids on the maxillae. We discuss the conservation status of both species. PMID:28761788

  5. Positivity for HLA DR1 is associated with basal cell carcinoma in renal transplant patients in southern Brazil.

    PubMed

    de Carvalho, André V E; Bonamigo, Renan R; da Silva, Cristina M; Pinto, Angela C De Zorzi

    2012-12-01

    Renal transplant patients have a higher incidence of non-melanoma skin cancer (NMSC). Previous studies hypothesized that human leukocyte antigen (HLA), especially types DR1, DR4, and DR7, may influence the incidence of these tumors. This study investigates the association between NMSC and the presence of HLA DR1, DR4, and DR7 in renal transplant patients in southern Brazil. In a historical cohort study, 1032 patients who underwent renal transplantation during the period from January 1993 to December 2006 were examined to identify occurrences of NMSC and HLA status prior to transplant. Of the 1032 patients examined, 59 (5.71%) developed NMSC (squamous cell carcinoma [SCC]: 2.42%; basal cell carcinoma [BCC]: 1.74%; both: 1.55%). The presence of HLA DR1 was associated with a higher probability of developing any NMSC and particularly with developing BCC (P < 0.05). There was no statistically significant association between the presence of HLA DR4 or DR7 and the occurrence of NMSC in this sample. HLA DR1 appears to be associated with the development of BCC, as well as with the higher number of NMSC lesions in renal transplant patients. This study supports the trend to associate the DR1 allele with BCC and not with SCC. © 2012 The International Society of Dermatology.

  6. Aliphatic and polycyclic aromatic hydrocarbons in surface sediments collected from mangroves with different levels of urbanization in southern Brazil.

    PubMed

    Assunção, Maíra Algarve; Frena, Morgana; Santos, Ana Paula Stein; Dos Santos Madureira, Luiz Augusto

    2017-06-15

    Three mangroves located in southern Brazil, Carijós (CA), Rio Tavares (RT) and Itacorubi (ITA), with distinct anthropogenic influences, were assessed with regard to the presence of aliphatic and polycyclic aromatic hydrocarbons (PAH). In this study, the n-alkane concentrations ranged from 1.9μg g(-1) (CA) to 55.6μg g(-1) (ITA) (dry weight). The carbon preference index (CPI) ranged from 2.1 to 7.9 and values for the terrestrial/aquatic ratio (TAR) were >1. Thus, both indexes indicated the predominance of sediment of terrestrial origin, mainly comprised of higher plants. Concentrations of total PAH ranged from 6.8ng g(-1) (RT) to 437.3ng g(-1) (ITA). The PAH isomeric ratios indicated that these compounds originated mainly from pyrogenic sources. Nevertheless, levels of n-alkanes in the three mangroves were relatively low and they are considered typical of uncontaminated surface sediments, while the level of contamination with PAH was classified as low to moderate. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Sympatry between Alouatta caraya and Alouatta clamitans and the rediscovery of free-ranging potential hybrids in Southern Brazil.

    PubMed

    Aguiar, Lucas M; Mellek, Daniel M; Abreu, Kaue C; Boscarato, Tiago G; Bernardi, Itiberê P; Miranda, João M D; Passos, Fernando C

    2007-07-01

    Records of sympatry between Alouatta caraya and A. clamitans are rare despite their extensive range overlap. An example of their current sympatry and the rediscovery of free-ranging potential hybrids of A. caraya and A. clamitans in the forests of the Upper Paraná River, Southern Brazil, are reported in this paper. Eight groups were observed in the study area: five monospecific groups of A. caraya, two of A. clamitans, and a group containing two adult males and two adult females of A. caraya and a sub-adult male and two adult females identified as Alouatta sp. The color of the last three individuals was a mosaic between the two species; this is consistent with previously described variations in museum specimens collected in the Paraná River in the 1940s that had been identified as potential hybrids. The results from this study emphasize the need for scientific studies in the region of the Ilha Grande National Park, one of the few regions in the Paraná River that currently harbors both howler species.

  8. Vitamin D Status and VDR Genotype in NF1 Patients: A Case-Control Study from Southern Brazil

    PubMed Central

    Souza Mario Bueno, Larissa; Rosset, Clévia; Aguiar, Ernestina; Pereira, Fernando de Souza; Izetti Ribeiro, Patrícia; Scalco, Rosana; Matzenbacher Bittar, Camila; Brinckmann Oliveira Netto, Cristina; Gischkow Rucatti, Guilherme; Chies, José Artur; Camey, Suzi Alves; Ashton-Prolla, Patricia

    2015-01-01

    Neurofibromatosis type 1 (NF1) patients are more likely to have vitamin D deficiency when compared to the general population. This study aimed to determine the levels of 25-OH-vitamin D [25(OH)D] in individuals with NF1 and disease-unaffected controls and analyze FokI and BsmI VDR gene polymorphisms in a case and in a control group. Vitamin D levels were compared between a group of 45 NF1 patients from Southern Brazil and 45 healthy controls matched by sex, skin type, and age. Genotypic and allelic frequencies of VDR gene polymorphisms were obtained from the same NF1 patients and 150 healthy controls. 25(OH)D deficiency or insufficiency was not more frequent in NF1 patients than in controls (p = 0.074). We also did not observe an association between FokI and BsmI VDR gene polymorphisms and vitamin D levels in NF1 patients, suggesting that their deficient or insufficient biochemical phenotypes are not associated with these genetic variants. The differences between the groups in genotypic and allelic frequencies for FokI and BsmI VDR gene polymorphisms were small and did not reach statistical significance. These polymorphisms are in partial linkage disequilibrium and the haplotype frequencies also did not differ in a significant way between the two groups (p = 0.613). PMID:26161090

  9. Mercury in sediments from gold and copper exploitation areas in the Camaquã River Basin, Southern Brazil

    NASA Astrophysics Data System (ADS)

    Pestana, M. H. D.; Lechler, P.; Formoso, M. L. L.; Miller, J.

    2000-11-01

    Mercury concentrations were determined in stream sediments from the Camaquã River Basin, located in the shield region of the state of Rio Grande do Sul, southern Brazil. The resulting geochemical data show that overbank floodplain deposits exhibit higher concentrations than sediments collected from the active channel bed. In addition, higher Hg concentrations were measured in the fine(<63 μm) sediment fraction of the samples. Total Hg concentrations in the fine fraction of active stream sediments from Lavras do Sul County, which have been influenced by past gold mining activities, have decreased during the last five years to values ≤142 ng g -1. However, in a settling pond containing abandoned mine wastes, the Hg concentration of a bulk sample remained exceptionally high (5220 ng g -1). Preliminary speciation results show that Hg 0 is the predominant species in most of the samples. This was the form of Hg released by the gold amalgamation activities in the area, and appears to be relatively stable under the existing Eh and pH conditions.

  10. Use of bioindicators to evaluate air quality and genotoxic compounds in an urban environment in Southern Brazil.

    PubMed

    Käffer, Márcia Isabel; Lemos, Andréa T; Apel, Miriam Anders; Rocha, Jocelita Vaz; Martins, Suzana Maria de Azevedo; Vargas, Vera Maria Ferrão

    2012-04-01

    Biological indicators are widely used to monitor genotic compounds and air quality in urban environments. Parmotrema tinctorum and Teloschistes exilis have been used to verify the presence of pollutants and analyze morphophysiological alterations in the thallus of species caused by their action. Species were exposed for seven months, in an urban area, in southern Brazil. Mutagenicity and cytotoxicity of PM10 organic extracts were assessed in the Salmonella/microsome assay at two stations. High concentrations of S, Pb, Cr, Zn and Hg were registered in the last period of exposure and more significant morphophysiological damages were verified in the lichens. Generally a higher mutagenic activity is observed in organic extracts of airborne particulate matter during the first months and in the third period of exposure of lichens. In addition, nitro compounds was detected through nitro-sensitive strains. Lichens and mutagenic biomarkers enabled the evaluation of air quality and the presence of environmentally-aggressive compounds. Copyright © 2011. Published by Elsevier Ltd.

  11. Molecular detection of bovine coronavirus in a diarrhea outbreak in pasture-feeding Nellore steers in southern Brazil.

    PubMed

    Ribeiro, Juliane; Lorenzetti, Elis; Alfieri, Alice Fernandes; Alfieri, Amauri Alcindo

    2016-03-01

    Worldwide diarrhea outbreaks in cattle herds are more frequently detected in calves being that diarrhea outbreaks in adult cattle are not common. Winter dysentery (WD) is a bovine coronavirus (BCoV) enteric infection that is more reported in Northern hemisphere. Seasonal outbreaks of WD in adult cattle occur mainly in dairy cows. WD has not been described in beef cattle herds of tropical countries. This study describes the molecular detection of BCoV in a diarrhea outbreak in beef cattle steers (Nellore) raised on pasture in Parana, southern Brazil. During the outbreak, the farm had about 600 fattening steers. Watery and bloody diarrhea unresponsive to systemic broad-spectrum antibiotic therapy reveals a morbidity rate of approximately 15 %. The BCoV N gene was identified in 42.9 % (6/14) of the diarrheic fecal samples evaluated by semi-nested polymerase chain reaction (SN-PCR) technique. Other enteric microorganisms occasionally identified in adult cattle and evaluated in this study such as bovine groups A, B, and C rotavirus, bovine viral diarrhea virus, bovine torovirus, aichivirus B, and Eimeria sp. were not identified in the fecal samples. To the best knowledge of the authors, this is the first description of the BCoV diagnosis in fecal samples collected in a diarrhea outbreak in adult beef cattle grazing in the grass in a tropical country.

  12. Ethnobotanical study of plants used for therapeutic purposes in the Atlantic Forest region, Southern Brazil.

    PubMed

    Tribess, Bianca; Pintarelli, Gabrielli Melatto; Bini, Larissa Alida; Camargo, Anderson; Funez, Luís Adriano; de Gasper, André Luís; Zeni, Ana Lúcia Bertarello

    2015-04-22

    Atlantic Forest is a biome in dangerous situation and it lacks wider information on species with medicinal purposes used by people in this area. In this study an ethnobotanical survey was conducted in Apiúna district, Brazil with the goal of assessing traditional knowledge of medicinal plants used by rural communities in a region covered by Atlantic Forest. The ethnobotanical data were collected through semi-structured interviews and a free list of plants used for medicinal purposes. The respondents were selected by snow ball method. Therefore, the therapeutic use of plants was investigated and the species cited was collected and identified. Local plant uses were evaluated using ethnobotanical indices of diversity and equitability, and then compared with those obtained in other regions of Atlantic Forest in Brazil. Besides, the informant consensus factor (ICF) was calculated. A total of 162 species belonging to 61 families were recorded, mainly Asteraceae and Lamiaceae. Furthermore, the species cited, 45.06% were native and 54.94% were considered exotic. The most frequently reported medicinal uses were the symptoms and signs (17.42%), digestive system (15.33%) and, infectious and parasitic diseases (12.73%). Although, the ICF calculation showed that mental and behavioral (0.85), respiratory system (0.79) and, digestive and genitourinary system diseases (0.78 for both) were the categories with higher values reached. Usually, the administration is oral from leaves preparations. Folk medicine in rural communities in this region of Atlantic Forest is an important source of primary health care. The results indicate an available knowledge of medicinal plants uses in this area, when compared to other regions previously studied. The fact that this research was conducted next to a conservation area makes it possible to dispose the knowledge organized here into a tool for environmental education as well as preservation. Moreover, the pharmacological information will further

  13. Chewing lice (Phthiraptera) from Calidris fuscicollis (Aves: Scolopacidae) in Southern Brazil.

    PubMed

    Gomes, Sâmara Nunes; Pesenti, Tatiana Cheuiche; Cirne, Maximiano Pinheiro; Müller, Gertrud

    2014-08-01

    During April and September from 2010 to 2012, 80 birds of the species Calidris fuscicollis (white-rumped sandpiper) were collected for parasitological studies in the southern coast of Rio Grande do Sul, under ICMBIO license No. 26234-1. For ectoparasite collection, the birds were first submerged in water with detergent. The parasites found were fixed in 70% alcohol, cleared in 10% potassium hydroxide and mounted in Canada balsam. Of 80 birds examined, 79% were parasitized. Actornithophilus umbrinus (47.5%), Actornithophilus lacustris (37.5%), Actornithophilus spp. (13.75%), Carduiceps zonarius (26.25%), Lunaceps incoenis (27.5%), and Lunaceps spp. (16.25%) were the species found with their respective prevalence. We record for the first time parasitism by chewing lice in Calidris fuscicollis. Copyright © 2014 Elsevier B.V. All rights reserved.

  14. Desmodus rotundus (Mammalia: Chiroptera) on the southern coast of Rio de Janeiro state, Brazil.

    PubMed

    Costa, L M; Esbérard, C E L

    2011-08-01

    Since the 1990s, attacks by hematophagous bats on humans and domestic animals have been reported both on the continent and on the islands on the southern coast of Rio de Janeiro state. The density of vampire bats was investigated based on percentage of captures during control of Desmodus rotundus samplings and during bat diversity research. In the present work, 203 individuals of D. rotundus were captured from 1993 to 2009, which corresponds to 11.88% of all bat captures carried out for species control in local villages and 1.58% of all captures in faunistic inventories. The density of D. rotundus is high even on the recently occupied islands where domestic animals have been introduced. It is probable that this species dispersed from the continent to the islands due to the introduction of domestic animals.

  15. Age distribution of Serra Geral (Paraná) flood basalts, southern Brazil

    USGS Publications Warehouse

    Fodor, R.V.; McKee, E.H.; Roisenberg, A.

    1989-01-01

    We evaluated 193 K-Ar ages (10 newly determined) of basaltic and differentiated rocks of the Serra Geral (Paraná) flood-basalt province for indications of magmatism occurring systematically with progressive rifting and complete separation ( ≈130-105 Ma) of South America from Africa. The K-Ar ages represent basalt emplacement between 35° and 19°S covering about 1,200,000 km2. We note that volcanism appears ubiquitous across the province between about 140 and 115 Ma, and that there are no significant age differences within that relate directly to progressive south-to-north tectonism. On the other hand, the oldest samples, about 140–160 Ma, are among those nearest the Brazil coastline (rift margin), perhaps suggesting migration of activity away from the rift with time. Studies of other flood-basalt provinces now indicate short (<3 m.y.) eruption periods, thereby pointing to the need for re-examination of Serra Geral ages by 40Ar-39Ar incremental heating techniques.

  16. Presence of OXA-23-producing isolates of Acinetobacter baumannii in wastewater from hospitals in southern Brazil.

    PubMed

    Ferreira, Alessandra E; Marchetti, Desirée P; De Oliveira, Lyvia M; Gusatti, Carolina S; Fuentefria, Daiane B; Corção, Gertrudes

    2011-06-01

    The aim of the study was to evaluate the dissemination of multiresistant isolates of Acinetobacter baumannii carrying resistance genes, by samples of wastewater from hospitals in Porto Alegre, Rio Grande do Sul, Brazil. We obtained 303 bacterial isolates from the wastewater of three hospitals in Porto Alegre, Rio Grande do Sul. For each isolate, we determined the profile of susceptibility to antimicrobials and the presence of the genes bla(OXA-23), bla(OXA-24), bla(OXA-51), bla(OXA-58), bla(SPM-1), bla(IMP), and bla(VIM.) The bla(OXA-51) gene was found in 56% of the isolates, indicating the presence of A. baumannii in this environment. Of these, three multiresistant isolates were positive for the bla(OXA-23) gene, in wastewater from two of the hospitals. The results obtained in this study indicate that isolates of A. baumannii which are multiresistant and carry resistance genes such as bla(OXA-51) and bla(OXA-23) are being released into the environment in the wastewater from the hospitals analyzed. Multiresistant Acinetobacter junii, the newly emerging pathogen, were also found among the multiresistant isolates. Hospital wastewater may be crucial to the development and dispersal of multiresistant bacteria, making waterbodies reservoirs of bacterial resistance.

  17. [Perception, knowledge, and use of generic drugs in southern Brazil: what changed from 2002 to 2012?].

    PubMed

    Guttier, Marília Cruz; Silveira, Marysabel Pinto Telis; Luiza, Vera Lucia; Bertoldi, Andréa Dâmaso

    2016-08-01

    This study compared the perception, knowledge, and use of generic drugs by adults in Pelotas, Rio Grande do Sul State, Brazil, using two cross-sectional population-based studies from 2002 and 2012. Study outcomes were: (a) prevalence of use of generics; (b) generics as a proportion of all medication; (c) users' perceptions of prices and quality; (d) users' knowledge of generics; and (e) strategies for acquisition of medicines. Prevalence of generics use increased from 3.6% (95%CI: 3.0-4.3) to 26.1% (95%CI: 24.5-27.7) in the 10-year period. Perceptions of prices and quality of generics remained stable, identification of characteristics that distinguish generics from other drugs improved (p < 0.001), and drug classification errors decreased (p < 0.001). There was a significant increase in acquiring medication by replacing prescribed drugs with generics. Between 2002 and 2012 there was an increase in knowledge and use of generics, while perception of lower prices and equivalent quality remained high.

  18. Trichomonas vaginalis PREVALENCE AND RISK FACTORS FOR WOMEN IN SOUTHERN BRAZIL

    PubMed Central

    AMBROZIO, Cíntia Lima; NAGEL, Andréia Saggin; JESKE, Sabrina; BRAGANÇA, Guilherme Cassão Marques; BORSUK, Sibele; VILLELA, Marcos Marreiro

    2016-01-01

    SUMMARY Trichomonas vaginalis infections have been associated with other diseases so that epidemiological studies of the parasite are important and help to prevent the spread of the disease. This study aimed to determine the prevalence of T. vaginalis in female patients of 19 counties in southwestern Rio Grande do Sul, Brazil. For diagnosis, was used direct examination, followed by applying a socio-epidemiological questionnaire. We analyzed 300 women and 9% were infected by Trichomonas vaginalis. The highest frequency occurred in women between 18 and 39 years old, single/divorced/widowed, whose family income was at one minimum wage or less, and they had not completed the primary school. Statistically significant risk factors were: women reporting two or more sexual partners in the last year were 3.3 times more likely to acquire the parasite, and those in use of oral contraceptives were 2.7 times more likely to have T. vaginalis. Importantly, 33% of the asymptomatic women were infected, and most of the negative results were from women presenting symptoms consistent with the infection. The findings emphasize that it is necessary to expand the knowledge of individuals about the disease, especially among women with the above mentioned risk factors and also to include the regular screening of Trichomonas vaginalis infections in health centers. PMID:27680166

  19. Dermatophytosis: a 16-year retrospective study in a metropolitan area in southern Brazil.

    PubMed

    Heidrich, Daiane; Garcia, Marcelo Rocha; Stopiglia, Cheila Denise Ottonelli; Magagnin, Cibele Massotti; Daboit, Tatiane Caroline; Vetoratto, Gerson; Schwartz, Joel; Amaro, Taís Guarienti; Scroferneker, Maria Lúcia

    2015-08-29

    Dermatophytoses are considered a public health problem. The objectives of this study were to determine the evolution of their prevalence in the metropolitan area of Porto Alegre, Brazil, and to analyze the dermatophyte species distribution according to body site and demographic characteristics of the patients. This work was a retrospective analysis of data from patients attending a tertiary care hospital during 1996-2011. There were 9,048 cases with cultures positive for dermatophytes. Trichophyton rubrum occurred in 59.6% of the cases, followed by Trichophyton interdigitale (34%), Microsporum canis (2.6%), Epidermophyton floccosum (1.5%), Microsporum gypseum (1.3%), and Trichophyton tonsurans (0.9%). The angular coefficients for T. interdigitale, E. floccosum, T. rubrum, and M. canis were +1.119, +0.211, -0.826 and -0.324% per year, respectively. Males presented higher prevalence of infection (79.3% versus 53.9%). Tinea unguium occurred in 48.5% of the cases, followed by tinea pedis (33.1%). T. rubrum was the predominant species in all regions of the body except the scalp, where M. canis was responsible for 75% of the cases. Monitoring of the evolution of dermatophytosis tracks changes in prevalence over the years and may assist practical measures for the public health control of this disease.

  20. [Study on the dental fluorosis in a Southern city of Brazil].

    PubMed

    Rigo, Lilian; Caldas Junior, Arnaldo de França; Souza, Eliane Alvim de; Abegg, Claides; Lodi, Leodinei

    2010-06-01

    The objective of this article is to determine the frequency and severity of dental fluorosis, as well as to verify possible associations with dental caries, gender, age, ethnicity and geographic location of schools in schoolchildren of 12 years old and from 15 to 19 years in the city of Passo Fundo, Rio Grande do Sul State, Brazil. A total of 633 schoolchildren of both genders at the cited ages, enrolled in 26 public schools of fundamental teaching were involved in the epidemiological study. The inspection was performed by three examiners previously trained to apply Dean's Index. The prevalence of dental fluorosis in the studied group was of 32.8%. Predominant degree was the very light (78.4%), followed by light (11.1%), questionable (8.7%) and moderate (1.9%). The prevalence of dental fluorosis was expressive in this population, however, with a low degree of severity. Factors such as age and dental caries index showed to be related to the variable response.

  1. Isolation and identification of Acanthamoeba spp. from thermal swimming pools and spas in Southern Brazil.

    PubMed

    Fabres, Laura Fuhrich; Rosa Dos Santos, Sayonara Peixoto; Benitez, Lisianne Brittes; Rott, Marilise Brittes

    2016-03-01

    Free-living amoebae (FLA) are widely distributed in soil and water. A few number of them are implicated in human disease: Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris and Sappinia diploidea. Species of Acanthamoeba can cause keratitis and brain infections. In this study, 72 water samples were taken from both hot tubs and thermal swimming pools in the city of Porto Alegre, RS, Brazil, to determine the presence of Acanthamoeba in the water as well as perform the phenotypic and genotypic characterization of the isolates. The identification of the isolates was based on the cysts morphology and PCR amplification using genus-specific oligonucleotides. When the isolates were submitted to PCR reaction only 8 were confirmed as belonging to the genus Acanthamoeba. The sequences analysis when compared to the sequences in the GenBank, showed genotype distribution in group T3 (12,5%), T5 (12,5%), T4 (25%) and T15 (50%). The results of this study confirmed the presence of potentially pathogenic isolates of free living amoebae in hot swimming pool and spas which can present risks to human health.

  2. Contextual analysis and epidemiology of spider bite in southern Santa Catarina State, Brazil.

    PubMed

    Cristiano, Maykon P; Cardoso, Danon C; Raymundo, Melissa S

    2009-09-01

    The aim of this work was to conduct an epidemiological and clinical review of 1126 cases of envenoming by spider recorded in the national notifiable diseases information system (SINAN) files of the Municipal Health Secretary of the Criciúma region, Santa Catarina State, Brazil from 1994 to 2006. This work presents a critical analysis carried out by the application of the chi(2) test, with different regional contextual parameters, including the incidence coefficient. Spider envenoming accounts for more than 50% of all cases recorded in the SINAN files for poisonous animals and is the main cause of human envenomation in the Criciúma region. The majority of the spider bites (57.4%) were reported in the hottest months, from December to May. The anatomic region that suffered the most bites was the hand. Nearly 50% of the accidents were recorded in the group aged 20-49 years. The spiders most frequently involved in accidents for all age groups were of the Loxosceles genus. Although the data collected lack further epidemiological and, especially, clinical details, this does not affect the conclusions of the study, which could be used in the planning of actions aimed at improving environmental health.

  3. Interflow, overland flow and leaching of natural nutrients on an Alfisol slope of southern Bahia, Brazil

    NASA Astrophysics Data System (ADS)

    De Oliveira Leite, J.

    1985-09-01

    Two experimental plots for hydrologic studies, 3595 and 7060 m 2, were delimited on a slope of Alfisol planted with cacao in Bahia, Brazil. Volumes of overland flow and interflow were measured daily and samples of collected water were taken monthly for analysis of Ca, Mg, Na, K, N, P and Fe. The highest overland-flow volumes represented 24% and the highest interflow 53% of the rainfall but on the average the volumes of overland flow and interflow were found to represent 1 and 14% of the annual rainfall. The percentage of interflow increases with increasing rainfall. In winter, it is higher than in summer, except during the highest rains observed. The mean annual loss for calcium was 85.8 kg ha -1 yr -1; for magnesium 18.2; potassium 17.0; sodium 23.5; nitrogen 22.1; iron 5.5 and phosphorus 0.9. In relative terms, considering the chemical components of the soils, the K losses are highest, indicating that this element is most leachable. The interflow volumes and the amounts of Na, K, N and P correlated at the 1% significance level in both plots. A general conclusion is that the leaching of nutrients varied with the intensity of the interflow, especially for Na, K, N and P, the leaching of nutrients through overland flow being of less importance.

  4. [Health conditions, lifestyles and occupational characteristics of teachers in a city in southern Brazil].

    PubMed

    dos Santos, Marcio Neres; Marques, Alexandre Carriconde

    2013-03-01

    Various health complications have been identified among teachers and there is little information available about their lifestyle habits. The scope of this study was to investigate health conditions, lifestyles and occupational characteristics of municipal teachers in Bagé in the south of Brazil. A cross-sectional study involving 414 teachers was conducted by asking the teachers to fill out a standardized questionnaire. Descriptive and bivariate analysis techniques were employed. Most teachers were females (96.1%) and the mean age was 40.1 years (SD 9.4). Average teaching experience was 12.4 years (SD 9.5); 59% of the teachers had a degree; they taught many working hours per week (31.7 hours, SD 10.5) and 62% were absent from work at least once in the last 12 months. Self-rated heath was good (38.5%); 62.5% of them were physically active; 32.3% were overweight and 14.4% obese. They consumed hardly any fruit and vegetables (79.6%), had average stress levels of 14.9 points (SD 6.6) and 20.3% reported hypertension. Self-reported health was associated with teaching time, absenteeism, physical activity, number of meals per day, stress levels and hypertension. The prevalence of teachers who rated their health negatively was low, however several lifestyle issues were identified, which can be prejudicial to the health of these individuals.

  5. Microbiological and physicochemical analysis of the coastal waters of southern Brazil.

    PubMed

    Moresco, V; Viancelli, A; Nascimento, M A; Souza, D S M; Ramos, A P D; Garcia, L A T; Simões, C M O; Barardi, C R M

    2012-01-01

    The aim of this study was to assess the impact of sewage discharge on coastal waters by evaluating the influence of physicochemical parameters on the presence of enteric microorganisms in seawater samples collected from 11 beaches in Florianopolis, Santa Catarina, Brazil, over a one-year period (August 2009 to July 2010). Samples were assessed for the presence of human adenoviruses (HAdV), polyomavirus (JCPyV), hepatitis A virus (HAV), and noroviruses (HuNoV GI and GII). Escherichia coli and physicochemical parameters (salinity, temperature, pH and dissolved oxygen) were also evaluated. From the 132 samples analyzed, 55% were positive for HAdV, 51.5% for HAV, 7.5% for HuNoV GI, 4.5% for HuNoV GII, and 3% for JCPyV. E. coli levels ranged from 8 to 1325 CFU/100mL at all sites. The overall results highlight the problem of sewage discharge into coastal waters and confirm that there is no correlation between viral presence and bacterial contamination.

  6. Oral granuloma gravidarum: a retrospective study of 41 cases in Southern Brazil

    PubMed Central

    CARDOSO, Juliana Andrade; SPANEMBERG, Juliana Cassol; CHERUBINI, Karen; de FIGUEIREDO, Maria Antonia Zancanaro; SALUM, Fernanda Gonçalves

    2013-01-01

    Granuloma gravidarum (GG) is an inflammatory lesion, which develops in the oral mucosa of pregnant women in response to chronic low-grade irritants, under the influence of hormonal factors. Objective: To characterize GG clinically by means of a retrospective study of the cases of the Oral Medicine Division, São Lucas Hospital, Brazil. Material and Methods: Cases of GG diagnosed between 1980 and 2012 were analyzed. Data were obtained referring to the age of the patients, lesion location, clinical features, as well as the presence of local irritants. The gestation period in which the lesion developed was also investigated. Results: Forty-one cases of GG were found. The lesions developed predominantly in the third trimester of pregnancy (51.22%) and the mean age of the patients was 28 years. Most GG was found in the gingiva (73.17%), was reddish color and had a mean diameter of 1.5 cm. Local irritants were involved in 75.6% of the cases. Conclusions: The hormonal conditions of pregnancy can have an impact on the oral cavity, predisposing the patient to inflammatory lesions such as GG. PMID:23857656

  7. Molecular phylogeny of Neotropical bioluminescent beetles (Coleoptera: Elateroidea) in southern and central Brazil.

    PubMed

    Amaral, D T; Arnoldi, F G C; Rosa, S P; Viviani, V R

    2014-08-01

    Bioluminescence in beetles is found mainly in the Elateroidea superfamily (Elateridae, Lampyridae and Phengodidae). The Neotropical region accounts for the richest diversity of bioluminescent species in the world with about 500 described species, most occurring in the Amazon, Atlantic rainforest and Cerrado (savanna) ecosystems in Brazil. The origin and evolution of bioluminescence, as well as the taxonomic status of several Neotropical taxa in these families remains unclear. In order to contribute to a better understanding of the phylogeny and evolution of bioluminescent Elateroidea we sequenced and analyzed sequences of mitochondrial NADH2 and the nuclear 28S genes and of the cloned luciferase sequences of Brazilian species belonging to the following genera: (Lampyridae) Macrolampis, Photuris, Amydetes, Bicellonycha, Aspisoma, Lucidota, Cratomorphus; (Elateridae) Conoderus, Pyrophorus, Hapsodrilus, Pyrearinus, Fulgeochlizus; and (Phengodidae) Pseudophengodes, Phrixothrix, Euryopa and Brasilocerus. Our study supports a closer phylogenetic relationship between Elateridae and Phengodidae as other molecular studies, in contrast with previous morphologic and molecular studies that clustered Lampyridae/Phengodidae. Molecular data also supported division of the Phengodinae subfamily into the tribes Phengodini and Mastinocerini. The position of the genus Amydetes supports the status of the Amydetinae as a subfamily. The genus Euryopa is included in the Mastinocerini tribe within the Phengodinae/Phengodidae.

  8. Antiplasmodial and Cytotoxic Activities of Toad Venoms from Southern Amazon, Brazil

    PubMed Central

    Banfi, Felipe Finger; Guedes, Karla de Sena; Andrighetti, Carla Regina; Aguiar, Ana Carolina; Debiasi, Bryan Wender; Noronha, Janaina da Costa; Rodrigues, Domingos de Jesus; Júnior, Gerardo Magela Vieira; Sanchez, Bruno Antonio Marinho

    2016-01-01

    The drug-resistance of malaria parasites is the main problem in the disease control. The huge Brazilian biodiversity promotes the search for new compounds, where the animal kingdom is proving to be a promising source of bioactive compounds. The main objective of this study was to evaluate the antiplasmodial and cytotoxic activity of the compounds obtained from the toad venoms of Brazilian Amazon. Toad venoms were collected from the secretion of Rhinella marina and Rhaebo guttatus in Mato Grosso State, Brazil. The powder was extracted at room temperature, yielding 2 extracts (RG and RM) and a substance (‘1’) identified as a bufadienolide, named telocinobufagin. Growth inhibition, intraerythrocytic development, and parasite morphology were evaluated in culture by microscopic observations of Giemsa-stained thin blood films. Cytotoxicity was determined against HepG2 and BGM cells by MTT and neutral red assays. The 2 extracts and the pure substance (‘1’) tested were active against chloroquine-resistant Plasmodium falciparum strain, demonstrating lower IC50 values. In cytotoxic tests, the 2 extracts and substance ‘1’ showed pronounced lethal effects on chloroquine-resistant P. faciparum strain and low cytotoxic effect, highlighting toad parotoid gland secretions as a promising source of novel lead antiplasmodial compounds. PMID:27658592

  9. Pathology and morphometry of Hystrichis acanthocephalicus (Nematoda) from Phimosus infuscatus (Pelecaniformes) in southern Brazil.

    PubMed

    Scheer, Simone; Macedo, Márcia Raquel Pegoraro; Soares, Mauro Pereira; Schramm, Camila Costa; Muller, Gertrud

    2017-01-26

    Species of Hystrichis are parasite nematodes of the digestive tract of aquatic birds in South America, Europe and Asia. In Brazil, Hystrichis acanthocephalicus has been reported in Phimosus infuscatus. There are few data on the morphometry of this species and there are no reports on pathological conditions that it causes. Therefore, the purpose of this study was to report morphometric data from H. acanthocephalicus and describe the pathological effects of this parasite on the Phimosus infuscatus proventriculus. Thirty gastrointestinal tracts of P. infuscatus were examined to search for nematodes and H. acanthocephalicus occurred in 83% of hosts. Were measured the total length and body width of males and females, and of their respective cuticular spines, esophagus, spicules and eggs, and the internal and external diameter of copulatory bursa. Histopathological examination revealed parasitic structures in the proventriculus from the lumen (anterior end) to the outer layers of the organ (intermediate and posterior parts), in which we observed inflammatory reaction with infiltration of heterophils, hemorrhage and hemosiderin. The results of this study of histopathology, morphometry and parasitological indices are the first ones reported to H. acanthocephalicus and should contribute to the identification and recognition in cases of outbreaks in the Neotropical region.

  10. The new Brazilian traffic code and some characteristics of victims in southern Brazil

    PubMed Central

    Liberatti, C; Andrade, S; Soares, D

    2001-01-01

    Objective—The aim was to study characteristics of traffic accident victims before and after the implementation of the new Brazilian traffic code, in January 1998. Subjects and methods—The study population was car and motorcycle occupants seen in a pre-hospital care service in Londrina, Paraná State (Brazil) before the introduction of the new Brazilian traffic code, from 22 January to 21 July 1997, and after its implementation during the same period in 1998. Victims were analyzed over the time periods according to helmet and seat belt use, gender, underage driving, and alcohol on the breath. Results—Use of seat belts increased from 45% to 62.6% and of helmets from 31.2% to 66.2% after the introduction of the new Brazilian code. The proportion driving under age 18 and with perceptible alcoholic breath declined significantly only among motorcycle riders. There was a 20% decline in car occupant injuries along with a 9% reduction in motorcycle related injuries after the change of the law. Conclusion—Results favor the hypothesis that rigorous legislation increases safer practices in traffic, at least during the first months of its implementation. PMID:11565982

  11. Foraging activity of the snail kite, Rostrhamus sociabilis (Aves: Accipitridae) in wetlands of southern Brazil.

    PubMed

    Bergmann, F B; Amaral, H L C; Pinto, D P; Chivittz, C C; Tozetti, A M

    2013-05-01

    The snail kite (Rostrhamus sociabilis) is widely distributed in the American continent. Its specialised diet consists mostly of the gastropod mollusk Pomacea sp and its foraging strategy probably varies depending on the season, prey availability, and climate factors, which can be reflected in its semi-nomad behaviour. This study was aimed at examining the hunting strategy of the snail kite, and its association with climate factors and habitat heterogeneity. Direct observations of birds between January 2010 and March 2011 in southernmost Brazil revealed that hunting was still the predominant foraging strategy (79% of records) to capture mollusks. Despite morphological specialisations to extract mollusks from the shells, the handling time (average = 92.4 s) was twice as much the time between prey search and capture (average = 55 s). The increase in the number of mollusks ingested apparently occurs when the resting time on perches or any other substrates near the hunting sites decreases between successive unsuccessful attempts. The correlation between the number of consumed preys and the climatic variables examined was low. Regarding habitat heterogeneity, our findings suggest that birds forage preferentially in marshes with low vegetation, which may increase the access to mollusks. The hunting efficiency of the snail kite was high (76 % successful attempts) compared to those of other birds of prey.

  12. Antiplasmodial and Cytotoxic Activities of Toad Venoms from Southern Amazon, Brazil.

    PubMed

    Banfi, Felipe Finger; Guedes, Karla de Sena; Andrighetti, Carla Regina; Aguiar, Ana Carolina; Debiasi, Bryan Wender; Noronha, Janaina da Costa; Rodrigues, Domingos de Jesus; Júnior, Gerardo Magela Vieira; Sanchez, Bruno Antonio Marinho

    2016-08-01

    The drug-resistance of malaria parasites is the main problem in the disease control. The huge Brazilian biodiversity promotes the search for new compounds, where the animal kingdom is proving to be a promising source of bioactive compounds. The main objective of this study was to evaluate the antiplasmodial and cytotoxic activity of the compounds obtained from the toad venoms of Brazilian Amazon. Toad venoms were collected from the secretion of Rhinella marina and Rhaebo guttatus in Mato Grosso State, Brazil. The powder was extracted at room temperature, yielding 2 extracts (RG and RM) and a substance ('1') identified as a bufadienolide, named telocinobufagin. Growth inhibition, intraerythrocytic development, and parasite morphology were evaluated in culture by microscopic observations of Giemsa-stained thin blood films. Cytotoxicity was determined against HepG2 and BGM cells by MTT and neutral red assays. The 2 extracts and the pure substance ('1') tested were active against chloroquine-resistant Plasmodium falciparum strain, demonstrating lower IC50 values. In cytotoxic tests, the 2 extracts and substance '1' showed pronounced lethal effects on chloroquine-resistant P. faciparum strain and low cytotoxic effect, highlighting toad parotoid gland secretions as a promising source of novel lead antiplasmodial compounds.

  13. Presence of Torque teno virus (TTV) in tap water in public schools from Southern Brazil.

    PubMed

    Vecchia, Andréia Dalla; Kluge, Mariana; dos Santos da Silva, Joseane V; Comerlato, Juliana; Rodrigues, Manoela T; Fleck, Juliane D; da Luz, Roger B; Teixeira, Thais F; Roehe, Paulo M; Capalonga, Roberta; Oliveira, Ana Beatriz; Spilki, Fernando R

    2013-03-01

    Torque teno virus (TTV) was surveyed in tap water collected in schools from three municipalities located in the south of Brazil. TTV genomes were found in 11.7 % (4/34) of the samples. TTV DNA was detected in 10.5 % (2/19) of the samples collected at the city of Caxias do Sul and in 25 % (2/8) of the samples from Pelotas. Those cities have a low rate of sewage treatment. All samples from Santa Cruz do Sul, which has nearly 92 % of its sewage treated, were negative. These results suggest that the amount of sewage treated may have an effect on the detection rates of TTV DNA in drinking water in a given urban area, showing a mild negative correlation (r = -0.76), when comparing the percentage of sewage treatment to the detection of TTV genomes. The detection rate of TTV was also compared with Escherichia coli, showing a strong correlation (r = 0.97), indicating that TTV may be a suitable marker of fecal contamination.

  14. Fluoroquinolone and macrolide resistance in Campylobacter jejuni isolated from broiler slaughterhouses in southern Brazil.

    PubMed

    Sierra-Arguello, Yuli M; Perdoncini, G; Morgan, R B; Salle, C T P; Moraes, H L S; Gomes, Marcos J P; do Nascimento, Vladimir Pinheiro

    2016-01-01

    Campylobacter jejuni is recognized as a leading cause of acute bacterial gastroenteritis in humans. The over-use of antimicrobials in the human population and in animal husbandry has led to an increase in antimicrobial-resistant infections, particularly with fluoroquinolones and macrolides. The aim of the present study was to provide information of the current status of antimicrobial resistance patterns in Campylobacter jejuni from poultry sources. Fifty strains were recovered from broiler slaughterhouses in Rio Grande do Sul state, Brazil, 2012. The strains were investigated for antimicrobial susceptibility against three agents (ciprofloxacin, nalidixic acid and erythromycin) by minimal inhibitory concentrations. The strains were analysed by polymerase chain reaction-restriction fragment length polymorphism for detection of the Thr-86 mutation that confers resistance to ciprofloxacin. In addition, all the strains were tested for the presence of efflux systems (cmeB gene) conferring antimicrobial resistance. The minimum inhibitory concentrations results showed that 98% of isolates were sensitive to erythromycin and most isolates were resistant to ciprofloxacin (94%) and nalidixic acid (90%). A complete correlation was observed between the minimum inhibitory concentrations and PCR-RFLP assay. Finally, the cmeB gene that is responsible for multidrug resistance was detected in 16 isolates out the 50 strains (32%).

  15. Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes.

    PubMed

    Raboni, Sonia Maria; Damasio, Guilherme Augusto Costa; Ferreira, Carla E O; Pereira, Luciane A; Nogueira, Meri B; Vidal, Luine R; Cruz, Cristina R; Almeida, Sergio M

    2014-07-01

    Viral acute gastroenteritis (AG) is a significant cause of hospitalisation in children younger than five years. Group A rotavirus (RVA) is responsible for 30% of these cases. Following the introduction of RVA immunisation in Brazil in 2006, a decreased circulation of this virus has been observed. However, AG remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. We conducted a prospective study of paediatric patients hospitalised for AG. Stool samples were collected to investigate human adenovirus (HAdV), RVA, norovirus (NoV) and astrovirus (AstV). NoV typing was performed by nucleotide sequencing and phylogenetic analysis. From the 225 samples tested, 60 (26%) were positive for at least one viral agent. HAdV, NoV, RVA and AstV were detected in 16%, 8%, 6% and 0% of the samples, respectively. Mixed infections were found in nine patients: HAdV/RVA (5), HAdV/NoV (3) and HAdV/NoV/RVA (1). The frequency of fever and lymphocytosis was significantly higher in virus-infected patients. Phylogenetic analysis of NoV indicated that all of these viruses belonged to genotype GII.4. The significant frequency of these pathogens in patients with AG highlights the need to routinely implement laboratory investigations.

  16. Nutrient recovery from swine waste and protein biomass production using duckweed ponds (Landoltia punctata): southern Brazil.

    PubMed

    Mohedano, R A; Velho, V F; Costa, R H R; Hofmann, S M; Belli Filho, P

    2012-01-01

    Brazil is one of the most important countries in pork production worldwide, ranking third. This activity has an important role in the national economic scenario. However, the fast growth of this activity has caused major environmental impacts, especially in developing countries. The large amount of nitrogen and phosphorus compounds found in pig manure has caused ecological imbalances, with eutrophication of major river basins in the producing regions. Moreover, much of the pig production in developing countries occurs on small farms, and therefore causes diffuse pollution. Therefore, duckweed pond have been successfully used in the swine waste polishing, generating further a biomass with high protein content. The present study evaluated the efficiency of two full scale duckweed ponds for the polishing of a small pig farm effluent, biomass yield and crude protein (CP) content. Duckweed pond series received the effluent from a biodigester-storage pond, with a flow rate of 1 m(3)/day (chemical oxygen demand rate = 186 kg/ha day) produced by 300 animals. After 1 year a great improvement of effluent quality was observed, with removal of 96% of total Kjeldahl nitrogen (TKN) and 89% of total phosphorus (TP), on average. Nitrogen removal rate is one of the highest ever found (4.4 g TKN/m(2) day). Also, the dissolved oxygen rose from 0.0 to 3.0 mg/L. The two ponds produced together over 13 tons of fresh biomass (90.5% moisture), with 35% of CP content, which represents a productivity of 24 tonsCP/ha year. Due to the high rate of nutrient removal, and also the high protein biomass production, duckweed ponds revealed, under the presented conditions, a great potential for the polishing and valorization of swine waste. Nevertheless, this technology should be better exploited to improve the sustainability of small pig farms in order to minimize the impacts of this activity on the environment.

  17. Ecology of a tick-borne spotted fever in southern Brazil.

    PubMed

    Krawczak, Felipe S; Binder, Lina C; Oliveira, Caroline S; Costa, Francisco B; Moraes-Filho, Jonas; Martins, Thiago F; Sponchiado, Jonas; Melo, Geruza L; Gregori, Fábio; Polo, Gina; Oliveira, Stefan V; Labruna, Marcelo B

    2016-10-01

    Rio Grande do Sul is the southernmost state of Brazil, bordering Uruguay. Clinical cases of spotted fever group (SFG) rickettsiosis were recently reported in Rio Grande do Sul. None of these cases was lethal, and all were confirmed by seroconversion to R. rickettsii antigens. Because serological cross-reactions are well known to occur between different SFG agents, the SFG agent responsible for the clinical cases remains unknown in Rio Grande do Sul, where no rickettsial agent is known to infect ticks. During 2013-2014, ticks and blood sera samples were collected from domestic dogs and wild small mammals, and from the vegetation in a SFG-endemic area of Rio Grande do Sul. Dogs were infested by Amblyomma ovale adult ticks, whereas small mammals were infested by immature stages of A. ovale, Ixodes loricatus, and adults of I. loricatus. Ticks collected on vegetation were adults of A. ovale, and immature stages of A. ovale, Amblyomma dubitatum, and Amblyomma longirostre. Three Rickettsia species were detected: Rickettsia bellii in I. loricatus, Rickettsia amblyommii in A. longirostre, and a Rickettsia parkeri-like agent (Rickettsia sp. strain Atlantic rainforest) in A. ovale. Seroreactivity to SFG antigens were detected in 19.7 % (27/137) canine and 37.5 % (15/40) small mammal sera, with highest titers to R. parkeri. Results indicate that the R. parkeri-like agent, strain Atlantic rainforest, is circulating between A. ovale ticks, dogs and small mammals in the study area, suggesting that this SFG pathogen could be one of the etiological agents of SFG clinical cases in Rio Grande do Sul.

  18. Factors Associated With Muscle Strength Among Rural Community-Dwelling Older Women in Southern Brazil.

    PubMed

    Confortin, Susana Cararo; Barbosa, Aline Rodrigues

    2015-01-01

    Muscle strength is an important predictor of disability, mortality, and health complications among older adults. The objective of this study was to verify the sociodemographic factors, lifestyle, and health conditions associated with inadequate muscle strength in older women in a rural community in the South of Brazil. This was a cross sectional study employing a probabilistic sample (2010-2011) of 270 older adult women (≥ 60 years). Handgrip strength (HGS) was measured with a dynamometer (Takei, Japan). Lower limb muscle strength (LLMS) was assessed through the "chair stand test." The variables analyzed were age, literacy, current work status, living arrangement, occupation throughout life, smoking, sitting time, number of morbidities, history of falls, and cognitive function. The prevalence ratio was used as a measure of association, by Poisson regression analysis (crude and adjusted). The mean age of the older women was 73.2 (standard deviation, 8.8) years. The proportion of women who presented with inadequate HGS and LLMS was 18.8% (95% confidence interval, 13.8-23.7) and 29.8% (95% confidence interval, 23.9-35.6), respectively. Inadequate strength in the 2 tests was associated with age, the condition of not working, and altered cognitive function. Inadequate strength in the LLMS test was associated with having fallen during the last year. Factors associated with muscle strength are specific to the test. The following sociodemographic and health variables were positively associated with inadequate strength on the HGS and LLMS tests: advancing age, not working, and altered cognitive function. A history of falls is associated with inadequate performance on the LLMS test.

  19. Occurrence of aggressive periodontitis in patients at a dental school in southern Brazil.

    PubMed

    Hermes, Chirley Roberta; Baumhardt, Simone Glesse; Rösing, Cassiano Kuchenbecker

    2013-01-01

    Aggressive periodontitis is a rare, severe and rapidly progressing periodontal disease. Early diagnosis is of utmost importance for establishing treatment in order to stop periodontal destruction and prevent tooth loss. The aim of this study is to describe the occurrence of aggressive periodontitis in patients at a Dental School in Brazil by means of a cross-sectional study. First, records from patients aged 15-36 years were consecutively scrutinized. Patients should not have systemic diseases. The search went up to 383 valid records. By means of periapical radiographs, the distance between the cement-enamel junction and the bone crest was measured. Records in which there was severe bone loss or periodontal destruction incompatible with the age of the patient were selected. Patients with bone loss > or = 3mm were called to answer a questionnaire and undergo periodontal examination, in order to confirm or dismiss the diagnosis of aggressive periodontitis. From a total 383 records, 55.1% (211) were female and 44.9% (172) were male. In 3.9% (15) of the records, presumed diagnosis was aggressive periodontitis, and 12 out of those 15 eligible patients (80%) came in for clinical examination and confirmation or dismissal of the diagnosis. Aggressive periodontitis was diagnosed in 7 patients, corresponding to 1.8% of the total. Of these, 4 (1% of the total) presented generalized aggressive periodontitis and 3 (0.8% of the total) presented localized aggressive periodontitis. In 5 patients (1.3%) chronic periodontitis was diagnosed. It may be concluded, within the limits of the study, that aggressive periodontitis at this Dental School is compatible with world prevalence values, suggesting the need for periodontal diagnosis as from adolescence, considering the possible damage caused by this disease.

  20. Dental pain prevalence and association with dental caries and socioeconomic status in schoolchildren, Southern Brazil, 2002.

    PubMed

    Nomura, Lincon Hideo; Bastos, João Luiz Dornelles; Peres, Marco Aurélio

    2004-01-01

    The objective of this study was to assess the relation between dental pain, dental caries and socioeconomic status among 12- and 13-year-old schoolchildren enrolled in a public school in Florianópolis, SC, Brazil in 2002. This study was a cross-sectional study involving 181 schoolchildren. Dental pain experience was the dependend variable analyzed. Socioeconomic data of the children's families were obtained through a questionnaire. Dental caries experience was registered according to the DMFT index (WHO, 1997). The field workteam consisted of an examiner and a recorder. The statistical analysis was performed using the chi-square test and the non-conditional multiple logistic regression. The response rate was 93.4%. The intraexaminer agreement measured on a tooth by tooth basis was high (kappa > 0.73). Dental pain prevalence was 33.7% (CI95% 26.0-42.0). The multiple regression analysis, adjusted by sex and other variables, showed that children with DMFT > 1 presented 2.9 (OR CI95% 1.4-6.1, p < 0.01) more chances of having dental pain when compared with those with DMFT < or = 1. Children whose mother's schooling level was equal or less than 4 years presented 2.5 (OR CI95% 1.2-5.6, p = 0.02) more chances of having dental pain when compared with others whose mothers had more than 5 years of schooling and, finally, children whose family income was up to U$ 67.00 showed 3.2 (OR CI95% 1.2-8.4, p = 0.02) more chances of having dental pain when compared with the ones whose families had higher income. High levels of caries attack, low mother schooling level and low family income were associated to dental pain.

  1. Isolation and identification of feline calicivirus and feline herpesvirus in Southern Brazil

    PubMed Central

    Henzel, Andréia; Brum, Mário Celso Sperotto; Lautert, Cláudia; Martins, Mathias; Lovato, Luciane Teresinha; Weiblen, Rudi

    2012-01-01

    Feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1) are the two primary causes of upper respiratory tract disease in cats. The aim of this study was to demonstrate the distribution of FCV and FHV-1 among the feline population of several counties in Rio Grande do Sul State, Brazil. To this end, conjunctival and nasal swabs were collected from 302 cats from different locations, including households, breeding catteries, veterinary clinics, animal hospitals and experimental research facilities. The samples were collected between July 2006 to June 2009. The virus isolation was performed in CRFK cells and, subsequently, the identification was confirmed by PCR. FCV, FHV-1, or both were isolated from 55 cats from 28 different locations. FCV alone was isolated from 52.7% (29/55) of the animals that tested positively, FHV-1 alone was isolated from 38.2% (21/55) of the animals that tested positively, and co-infection were detected in 9.1% (5/55) of the animals that tested positively. Virus detection was more prevalent in cats that were less than 1 year old, among animals that shared a living space with other cats, and females. FCV and FHV-1 were isolated from vaccinated cats. In addition, both viruses were isolated from cats that showed no signs of disease. The results suggest that a carrier state is common for both viruses in the evaluated population. A search for other causes of respiratory disease in that population is necessary; and further studies relating to the molecular characterization of viruses and vaccine efficacy are also necessary. PMID:24031864

  2. Post-collisional Ediacaran volcanism in oriental Ramada Plateau, southern Brazil

    NASA Astrophysics Data System (ADS)

    Matté, Vinícius; Sommer, Carlos Augusto; Lima, Evandro Fernandes de; Philipp, Ruy Paulo; Basei, Miguel Angelo Stipp

    2016-11-01

    Ediacaran volcanic sequences in southernmost Brazil are related to intense post-collisional magmatism of the Brasiliano Orogeny. A portion of this volcanism occurs in the oriental Ramada Plateau located in the center part of the Rio Grande Sul State and is correlated with Hilário and Acampamento Velho formations. The first one is represented dominantly by lava flows and dikes of shoshonitic andesitic composition, besides of volcanogenic sedimentary deposits. The acid rocks of the Acampamento Velho Formation are expressive in the area, comprising high-silica ignimbrites, usually densely welded. Dikes and domes are common too and rhyolitic lava flows occur at the top and intercalated to ignimbrites in the middle of the sequence. The acid rock association has a sodic alkaline affinity. In this unit we mapped a subvolcanic sill of trachyte showing evidence for magma mixing with the rhyolitic magma. It has sodic alkaline affinity, and FeOt/FeO + MgO ratios and agpaitic index lower than those recorded in the rhyolites/ignimbrites. The Acampamento Velho Formation includes in this area, subordinately, basalts as àà flows and dikes intercalated with acid rocks. They have sodic alkaline nature and characteristics of intraplate basic rocks. New zircon U-Pb dating indicates crystallization age of 560 ± 2 Ma in a densely welded ignimbrite, 560 ± 14 Ma for a mafic trachyte and 562 ± 2 Ma for a subvolcanic rhyolite. The sodic alkaline rocks in this region evolved by fractional crystallization processes and magma mixing with major crustal contribution at approximately 560 Ma. The chemical characteristics are similar to those of A-type granites associated with Neoproterozoic post-collision magmatism in the Sul-rio-grandense Shield.

  3. Landslide initiation in saprolite and colluvium in southern Brazil: Field and laboratory observations

    NASA Astrophysics Data System (ADS)

    Lacerda, Willy A.

    2007-06-01

    The weathering of granitic and gneissic rocks in tropical regions can reach depths of more than 100 m. In southeast Brazil there are situations where landslide initiation depends on the fluctuation of the groundwater level, on the impact of falling rocks and on intense rainfall, causing superficial slides. The fluctuation of groundwater induces cyclical variations of the pore water pressure, and consequently of the effective stresses. This variation causes cyclic expansion and contraction of the structure of the saprolitic soil, weakening the imbrication of grains and loss of the cementation that may exist. This could be called a "fatigue" phenomenon. The practical effect is the lowering of the Mohr shear strength envelope, and a sudden rupture of the soil at a lower groundwater level than that which would be compatible with the intact soil strength properties, initiating a landslide. Another situation arises during intense rains, when a rock slab or a rock block detaches from the uppermost parts of a slope. This occurs where steep rock outcrops exhibit relief joints or where residual blocks of granite roll down the slope, impacting the compressible, saturated colluvial soil overlying the saprolitic soil. The sudden increase of pore pressure can liquefy the soil. Finally, another mechanism is that of the advance of a saturation front in a steep slope of unsaturated saprolitic soil, reaching a depth below the root zone. The loss of the cohesion due to suction, without the beneficial contribution of the roots to the shearing strength, causes a sudden slide. During extreme rain episodes literally hundreds of such superficial slides, reaching 1 to 3 m in depth, occur in a given basin. The concentrated runoff that flows along the surface of the thalweg of the basin carries this soil in a muddy state, and a debris flow ensues.

  4. Abundance and species richness of Coreoidea (Hemiptera: Heteroptera) from Parque Estadual do Turvo, Southern Brazil.

    PubMed

    Barcellos, Aline; Schmidt, Letícia S; Brailovsky, Harry

    2008-01-01

    The coreoid fauna from Neotropics is poorly known, especially in terms of community studies. Aiming at contributing to this knowledge, a two-year study was carried out at Parque Estadual do Turvo, Municipality of Derrubadas, state of Rio Grande do Sul, Brazil, to evaluate the composition, abundance and species richness of Coreoidea. Samplings were conducted in the springs of 2003 and 2004 (October), and autumns of 2004 and 2005 (May), using beating tray method, along two trails of the park. Sampling effort (hours X collectors) totaled 153h. A total of 282 individuals of Coreoidea were collected, distributed in 28 species of Alydidae, Coreidae and Rhopalidae. The most abundant species was the coreid Cebrenis supina Brailovsky, representing 16% of the collected individuals, followed by the rhopalids Jadera aeola (Dallas), and Harmostes sp., with 12.1% and 11.7%, respectively. The estimated richnesses by Chao 1, Chao 2, Jackknife 1 and Jackknife 2 indicated that the observed richness corresponds to 70% to 80% of the expected for the area. The estimated richness through rarefaction was significantly higher in spring 2003 and autumn 2004 than in the other periods. There was no significant difference, however, between spring of 2003 and autumn of 2004, and between spring of 2004 and autumn of 2005, for the same parameter. Yucumã and Garcia trails did not differ significantly for the estimated richness. Singletons and doubletons represented 32.1% of the recorded species. Additionally, eight other species were obtained qualitatively by using, besides beating tray without protocol, manual collection.

  5. A new species of flea-toad (Anura: Brachycephalidae) from southern Atlantic Forest, Brazil.

    PubMed

    Condez, Thais Helena; Monteiro, Juliane Petry De Carli; Comitti, Estevão Jasper; Garcia, Paulo Christiano De Anchietta; Amaral, Ivan Borel; Haddad, Célio Fernando Baptista

    2016-02-18

    We describe a new species of Brachycephalus that is morphologically similar to the flea-toads B. didactylus, B. hermogenesi, and B. pulex. The new species occurs from the sea level up to 1000 m and it is widely distributed throughout southern Atlantic Forest. Brachycephalus sulfuratus sp. nov. is distinguished from all of its congeners by the combination of the following characters: (1) small body size (SVL of adults: 7.4-8.5 mm for males and 9.0-10.8 mm for females); (2) "leptodactyliform" body; (3) pectoral girdle arciferal and less robust compared to the Brachycephalus species with "bufoniform" body; (4) procoracoid and epicoracoid fused with coracoid but separated from the clavicle by a large fenestrae; (5) toe I externally absent; toes II, III, IV, and V distinct; phalanges of toes II and V reduced; (6) skin smooth with no dermal ossifications; (7) in life, general background color brown with small dark-brown spots; skin of throat, chest, arms, and forearms with irregular yellow blotches; in ventral view, cloacal region of alive and preserved specimens surrounded by a dark-brown inverted v-shaped mark outlined with white; (8) advertisement call long, composed of a set of 4-7 high-frequency notes (6.2-7.2 kHz) repeated regularly.

  6. Ectoparasites of the critically endangered insular cavy, Cavia intermedia (Rodentia: Caviidae), southern Brazil.

    PubMed

    Regolin, André Luis; Furnari, Nina; de Castro Jacinavicius, Fernando; Linardi, Pedro Marcos; de Carvalho-Pinto, Carlos José

    2015-04-01

    Cavia intermedia is a rodent species critically endangered and is found only on a 10 hectare island off the southern Brazilian coast. To identify the ectoparasites of C. intermedia, 27 specimens (14 males and 13 females), representing approximately 65% of the estimated total population, were captured and examined. A total of 1336 chewing lice of two species were collected: Gliricola lindolphoi (Amblycera: Gyropidae) and Trimenopon hispidum (Amblycera: Trimenoponidae). In addition, chiggers Arisocerus hertigi (Acari: Trombiculidae) and Eutrombicula sp. (Acari: Trombiculidae) were collected from the ears of all captured animals. This low species richness compared to those for other Cavia species is expected for island mammals. Although the results presented here are not conclusive about the relationship between C. intermedia and ectoparasites, this low species richness found might be reflected in a low level of investment by the hosts in the basal immune defense, since investments in white blood cell production by mammals are influenced by the diversity of parasites in the environment. Additionally, considering that it might result in host vulnerability to other parasites that might be introduced through exotic or migratory host species, the monitoring of C. intermedia, including parasitological and immunological assessments, is recommended as a key component of conservation efforts.

  7. Modeling habitat suitability of the invasive clam Corbicula fluminea in a Neotropical shallow lagoon, southern Brazil.

    PubMed

    Silveira, T C L; Gama, A M S; Alves, T P; Fontoura, N F

    2016-04-19

    This study aimed to model the habitat suitability for an invasive clam Corbicula fluminea in a coastal shallow lagoon in the southern Neotropical region (-30.22, -50.55). The lagoon (19km2, maximum deep 2.5m) was sampled with an Ekman dredge in an orthogonal matrix comprising 84 points. At each sampling point, were obtained environmental descriptors as depth, organic matter content (OMC), average granulometry (Avgran), and the percentage of sand (Pcsand). Prediction performance of Generalized Linear Models (GLM), Generalized Additive Models (GAM) and Boosted Regression Tree (BRT) were compared. Also, niche overlapping with other native clam species (Castalia martensi, Neocorbicula limosa and Anodontites trapesialis) was examined. A BRT model with 1400 trees was selected as the best model, with cross-validated correlation of 0.82. The relative contributions of predictors were Pcsand-42.6%, OMC-35.8%, Avgran-10.9% and Depth-10.8%. Were identified that C. fluminea occur mainly in sandy sediments with few organic matter, in shallow areas nor by the shore. The PCA showed a wide niche overlap with the native clam species C. martensi, N. limosa and A. trapesialis.

  8. Ectoparasites of the critically endangered insular cavy, Cavia intermedia (Rodentia: Caviidae), southern Brazil

    PubMed Central

    Regolin, André Luis; Furnari, Nina; de Castro Jacinavicius, Fernando; Linardi, Pedro Marcos; de Carvalho-Pinto, Carlos José

    2015-01-01

    Cavia intermedia is a rodent species critically endangered and is found only on a 10 hectare island off the southern Brazilian coast. To identify the ectoparasites of C. intermedia, 27 specimens (14 males and 13 females), representing approximately 65% of the estimated total population, were captured and examined. A total of 1336 chewing lice of two species were collected: Gliricola lindolphoi (Amblycera: Gyropidae) and Trimenopon hispidum (Amblycera: Trimenoponidae). In addition, chiggers Arisocerus hertigi (Acari: Trombiculidae) and Eutrombicula sp. (Acari: Trombiculidae) were collected from the ears of all captured animals. This low species richness compared to those for other Cavia species is expected for island mammals. Although the results presented here are not conclusive about the relationship between C. intermedia and ectoparasites, this low species richness found might be reflected in a low level of investment by the hosts in the basal immune defense, since investments in white blood cell production by mammals are influenced by the diversity of parasites in the environment. Additionally, considering that it might result in host vulnerability to other parasites that might be introduced through exotic or migratory host species, the monitoring of C. intermedia, including parasitological and immunological assessments, is recommended as a key component of conservation efforts. PMID:25830106

  9. Edge effects on epigeic ant assemblages in a grassland-forest mosaic in southern Brazil

    NASA Astrophysics Data System (ADS)

    Pinheiro, Esther R. S.; Duarte, Leandro da S.; Diehl, Elena; Hartz, Sandra M.

    2010-07-01

    This study analyzed the influence of vegetation structure variation along a natural vegetation mosaic formed by Araucaria forest and Campos grassland in the southern Brazilian highlands, on the species richness and composition of epigeic ants. The study site consisted of two different grassland-forest ecotones, where 76 pitfall traps were installed. We estimated the area covered by canopy vegetation by hemispherical photographs, and the structure of the understory vegetation by counting the number of vegetation touches, using a graduated stick. We collected 31 species or morphospecies of epigeic ants belonging to 17 genera and 6 subfamilies. Cluster analysis defined three habitat groups (grassland, edge, and forest) with different ant species composition as revealed by ordination analysis. The highest richness was observed at the forest edge, and decreased towards the grassland and the forest interior. Variation in the richness and composition of epigeic ant species was significantly explained by the factor of distance from the forest. The relationship between species richness and understory density was negative. On the other hand, species composition of epigeic ant assemblages was significantly explained by canopy cover. This finding indicates that the ecological responses of ant assemblages resulted predominantly from edge effects mediated by changes in vegetation structure.

  10. Pharmacy practice simulations: performance of senior pharmacy students at a University in southern Brazil

    PubMed Central

    Galato, Dayani; Alano, Graziela M.; Trauthman, Silvana C.; França, Tainã F.

    Objective A simulation process known as objective structured clinical examination (OSCE) was applied to assess pharmacy practice performed by senior pharmacy students. Methods A cross-sectional study was conducted based on documentary analysis of performance evaluation records of pharmacy practice simulations that occurred between 2005 and 2009. These simulations were related to the process of self-medication and dispensing, and were performed with the use of patients simulated. The simulations were filmed to facilitate the evaluation process. It presents the OSCE educational experience performed by pharmacy trainees of the University of Southern Santa Catarina and experienced by two evaluators. The student general performance was analyzed, and the criteria for pharmacy practice assessment often identified trainees in difficulty. Results The results of 291 simulations showed that students have an average yield performance of 70.0%. Several difficulties were encountered, such as the lack of information about the selected/prescribed treatment regimen (65.1%); inadequate communication style (21.9%); lack of identification of patients’ needs (7.7%) and inappropriate drug selection for self-medication (5.3%). Conclusions These data show that there is a need for reorientation of clinical pharmacy students because they need to improve their communication skills, and have a deeper knowledge of medicines and health problems in order to properly orient their patients. PMID:24367467

  11. Paleoproterozoic andesitic volcanism in the southern Amazonian craton (northern Brazil); lithofacies analysis and geodynamic setting

    NASA Astrophysics Data System (ADS)

    Roverato, Matteo; Juliani, Caetano; Capra, Lucia; Dias Fernandes, Carlos Marcelo

    2016-04-01

    Precambrian volcanism played an important role in geological evolution and formation of new crust. Most of the literature on Precambrian volcanic rocks describes settings belonging to subaqueous volcanic systems. This is likely because subaerial volcanic rocks in Proterozoic and Archean volcano-sedimentary succession are poorly preserved due to erosive/weathering processes. The late Paleoproterozoic Sobreiro Formation (SF) here described, seems to be one of the rare exceptions to the rule and deserves particular attention. SF represents the subaerial expression of an andesitic magmatism that, linked with the upper felsic Santa Rosa F., composes the Uatumã Group. Uatumã Group is an extensive magmatic event located in the Xingú region, southwestern of Pará state, Amazonian Craton (northern Brazil). The Sobreiro volcanism is thought to be related to an ocean-continent convergent margin. It is characterized by ~1880 Ma well-preserved calc-alkaline basaltic/andesitic to andesitic lava flows, pyroclastic rocks and associated reworked successions. The superb preservation of its rock-textures allowed us to describe in detail a large variety of volcaniclastic deposits. We divided them into primary and secondary, depending if they result from a direct volcanic activity (pyroclastic) or reworked processes. Our study reinforces the importance of ancient volcanic arcs and rocks contribution to the terrestrial volcaniclastic sedimentation and evolution of plate tectonics. The volcanic activity that produced pyroclastic rocks influenced the amount of detritus shed into sedimentary basins and played a major role in the control of sedimentary dispersal patterns. This study aims to provide, for the first time, an analysis of the physical volcanic processes for the subaerial SF, based in field observation, lithofacies analysis, thin section petrography an