Science.gov

Sample records for 200c 300 400

  1. 76 FR 12619 - Airworthiness Directives; The Boeing Company 737-200, -200C, -300, -400, and -500 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-08

    ...-18-06, Amendment 39-13784 (69 FR 54206, September 8, 2004), for certain Model 737-200, -200C, -300... 12866, (2) Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034...), 40113, 44701. Sec. 39.13 2. The FAA amends Sec. 39.13 by removing amendment 39-13784 (69 FR...

  2. 76 FR 54399 - Airworthiness Directives; The Boeing Company Model 737-200, -200C, -300, -400, and -500 Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-01

    ... 2010-01-09, Amendment 39-16167 (75 FR 1527, January 12, 2010), for certain Model 737-300, -400, and... a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26...-09, Amendment 39-16167 (75 FR 1527, January 12, 2010), and adding the following new AD: The...

  3. 76 FR 72858 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-28

    ... (69 FR 23646, April 30, 2004), for all Boeing Model 737-200C series airplanes. That AD requires... decompression of the airplane. On July 23, 2009, we issued AD 2009-16-14, Amendment 39-15987 (74 FR 38901... Since we issued AD 2004-09-09, Amendment 39-13598 (69 FR 23646, April 30, 2004), we have...

  4. 75 FR 81427 - Airworthiness Directives; The Boeing Company Model 747-200C, -200F, -400, -400D, and -400F Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ..., Amendment 39-14506 (71 FR 12122, March 9, 2006). The existing AD applies to certain Model 747-200C, -200F... (71 FR 12122, March 9, 2006), the Director of the Federal Register approved the incorporation by..., 2010 (75 FR 13046). That NPRM proposed to continue to require repetitive inspections for cracks in...

  5. The Chemical Interplay of Serpentinization, Methanogenesis, and Carbonate Formation at 200C and 300 bar

    NASA Astrophysics Data System (ADS)

    Jones, L.; Rosenbauer, R.; Oze, C.

    2008-12-01

    Serpentinization (olivine hydrolysis) is the primary process contributing to abiotic generation of elemental hydrogen (H2) in active mid-ocean ridge hydrothermal systems and forearc environments. Carbon dioxide in these types of systems reacts with serpentinization-derived H2 to produce methane (CH4) and with cations in seawater (Ca2+, Mg2+, Fe2+) to precipitate carbonate minerals. Despite a growing body of field and experimental evidence, the reaction pathways, kinetics, and geochemical interplay of serpentinization, methanogenesis and carbonate formation are not fully understood. Here, we evaluated the reaction kinetics of CH4 versus carbonate genesis during serpentinization of average Earth olivines (Fo~90) under pressure-temperature (PT) conditions similar to mid-ocean ridge and forearc environments. Laboratory experiments were preformed at identical T and P conditions (200°C and 300 bar) using flexible gold reaction-cells in hydrothermal autoclaves located in the Water- Rock Interaction Laboratory at the U.S. Geological Survey in Menlo Park, CA. The experiments demonstrate that CH4 production was minimal in CO2-rich fluids and that CH4 generation was enhanced in a CO2-limited experiment producing an end-member CH4 concentration of 44 μM after 860 hours. Equilibrium thermodynamics and reaction rates observed in these experiments demonstrate that carbonate precipitation is favored in CO2-saturated systems and methanogenesis is hindered. These results highlight the competition between methanogenesis and carbonate production in hydrothermal environments leading to the conclusion that the production of CH4 is greater when the concentration of CO2 is undersaturated with respect to carbonate equilibrium.

  6. 75 FR 1533 - Airworthiness Directives; The Boeing Company Model 747-200F, 747-200C, 747-400, 747-400D, and 747...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-12

    ..., 2004, as of April 6, 2006 (71 FR 10605, March 2, 2006). ADDRESSES: For service information identified..., Amendment 39-14499 (71 FR 10605, March 2, 2006). The existing AD applies to all Model 747-200F, 747-200C... July 23, 2009 (74 FR 36417). That NPRM proposed to continue to require repetitive inspections...

  7. 76 FR 6529 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-07

    ...We are adopting a new airworthiness directive (AD) for the products listed above. This AD requires installing two warning level indicator lights on the P2-2 center instrument panel in the flight compartment for certain airplanes. For a certain other airplane, this AD requires activating the cabin altitude warning and takeoff configuration warning lights. For all airplanes, this AD also......

  8. 75 FR 48620 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-11

    ... flightcrew due to hypoxia (lack of oxygen in body), and consequent loss of control of the airplane. DATES: We... warning horn could result in incapacitation of the flightcrew due to hypoxia (lack of oxygen in body), and... November 7, 2003, we issued related AD 2003-03-15 R1, Amendment 39-13366 (68 FR 64802, November 17,...

  9. 75 FR 9137 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-01

    ... energy aspects of this proposed AD. We will consider all comments received by the closing date and may... November 24, 2003, we issued AD 2003-24-08, Amendment 39-13377 (68 FR 67027, December 1, 2003), for all... Average U.S.- Action Work hours labor rate Parts Cost per airplane registered Fleet cost per...

  10. 75 FR 43803 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-27

    ..., 2010. On December 4, 2003 (68 FR 67027, December 1, 2003), the Director of the Federal Register... include an AD that supersedes AD 2003-24-08, Amendment 39-13377 (68 FR 67027, December 1, 2003). The... published in the Federal Register on March 1, 2010 (75 FR 9137). That NPRM proposed to continue to...

  11. 76 FR 74667 - Airworthiness Directives; The Boeing Company Model 737-200, -200C, -300, -400, and -500 Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-01

    ... publication listed in this AD as of February 16, 2010 (75 FR 1527, January 12, 2010). ADDRESSES: For service... (NPRM) to amend 14 CFR part 39 to supersede AD 2010-01-09, Amendment 39-16167 (75 FR 1527, January 12... September 1, 2011 (76 FR 54399). That NPRM proposed to continue to require repetitive external...

  12. 76 FR 3856 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-21

    ..., replacing the fuel pump power control relays for the main, center and auxiliary tanks, as applicable, with...'' (66 FR 23086, May 7, 2001). In addition to new airworthiness standards for transport airplanes and new... fuel boost pumps, the center tank fuel boost pumps, and for certain airplanes, the auxiliary tank...

  13. 76 FR 33988 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-10

    ... changes, replacing the fuel pump power control relays for the main, center, and auxiliary tanks, as... (76 FR 3856). That NPRM proposed to require, depending on airplane configuration, doing certain wiring changes, replacing the fuel pump power control relays for the main, center, and auxiliary tanks,...

  14. 76 FR 63229 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-12

    ... System Design Review, Flammability Reduction and Maintenance and Inspection Requirements'' (66 FR 23086.... Other Relevant Rulemaking On April 18, 2001, we issued AD 2001-08-24, Amendment 39-12201 (66 FR 20733... 16, 2009, we issued AD 2008-10-09 R1, Amendment 39- 16148 (74 FR 69264, December 31, 2009), for...

  15. 75 FR 19564 - Airworthiness Directives; The Boeing Company Model 737-200, -200C, -300, -400, and- 500 Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-15

    ..., are necessary to comply with AD 90-06-02, Amendment 39-6489 (55 FR 8372, March 7, 1990). FAA's... Order 12866, 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR... surrounding structure under the number 3 very high frequency (VHF) antenna, and corrective actions...

  16. 75 FR 10658 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-09

    ... 2006-07-12, Amendment 39-14539 (71 FR 16211, March 31, 2006). The existing AD applies to all Model 737... Register on May 20, 2009 (74 FR 23664). That NPRM proposed to expand the area to be inspected and, for..., Revision 2, dated October 25, 2006, mandated by AD 2003-14-06, Amendment 39-13225 (68 FR 42956, July...

  17. 76 FR 47522 - Airworthiness Directives; The Boeing Company Model 737-100, -200, -200C, -300, -400, and -500...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-05

    .... Discussion On June 5, 1995, we issued AD 95-12-17, amendment 39-9268 (60 FR 36981, July 19, 1995), for... produced with outboard chords at BS 727 made of 7075-T6 aluminum; subsequent airplanes were produced with outboard chords made of 7075-T73 aluminum. In addition, we have determined that the inspections of...

  18. 17 CFR 300.400 - Satisfaction of customer claims for standardized options.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Satisfaction of a “Claim for Cash” or a “Claim for Securities” Source: Sections 300.500 through 300.503 appear... 17 Commodity and Securities Exchanges 4 2014-04-01 2014-04-01 false Satisfaction of customer claims for standardized options. 300.400 Section 300.400 Commodity and Securities Exchanges...

  19. Westinghouse Hanford Company effluent report for 300, 400, and 1100 Area operations for calendar year 1989

    SciTech Connect

    McCarthy, M.J.

    1990-09-01

    The report tabulates both radioactive and nonradioactive liquid and airborne effluent data for 300, 400, and 1100 Area operations at the Hanford Site. The 300 Area is primarily a research and development area. The 400 Area houses the Fast Flux Test Facility. The 1100 Area contains central stores and vehicle maintenance facilities. Releases to the environment from Westinghouse Hanford Company operations within these areas during calendar year 1989 were both consistent with previous years and within regulatory limits. 2 refs., 10 tabs.

  20. 17 CFR 300.400 - Satisfaction of customer claims for standardized options.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Satisfaction of customer... CORPORATION Closeout Or Completion of Open Contractual Commitments § 300.400 Satisfaction of customer claims... determining what a customer will receive in either (1) a liquidation proceeding pursuant to the Act or (2)...

  1. 17 CFR 300.400 - Satisfaction of customer claims for standardized options.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Satisfaction of customer... CORPORATION Closeout Or Completion of Open Contractual Commitments § 300.400 Satisfaction of customer claims... determining what a customer will receive in either (1) a liquidation proceeding pursuant to the Act or (2)...

  2. 17 CFR 300.400 - Satisfaction of customer claims for standardized options.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 17 Commodity and Securities Exchanges 3 2012-04-01 2012-04-01 false Satisfaction of customer... CORPORATION Closeout Or Completion of Open Contractual Commitments § 300.400 Satisfaction of customer claims... determining what a customer will receive in either (1) a liquidation proceeding pursuant to the Act or (2)...

  3. 17 CFR 300.400 - Satisfaction of customer claims for standardized options.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 17 Commodity and Securities Exchanges 3 2013-04-01 2013-04-01 false Satisfaction of customer... CORPORATION Closeout Or Completion of Open Contractual Commitments § 300.400 Satisfaction of customer claims... determining what a customer will receive in either (1) a liquidation proceeding pursuant to the Act or (2)...

  4. Westinghouse Hanford Company effluent report for 300 and 400 area operations for calendar year 1987

    SciTech Connect

    McCarthy, M.J.

    1989-02-01

    Releases from the facilities in the 300 and 400 Areas remain well within the applicable administrative control values (ACV). No significant trends were noted in either the airborne or liquid effluent data. Airborne and liquid radioactive effluents constitute a major portion of the effluent sampling and monitoring program. Radioactive releases from facilities in the 300 and 400 Areas, which are operated by Westinghouse Hanford, totaled 36.5 Ci. Noble gases released from the Fast Flux Test Facility (FFTF) represented 36.3 Ci of this total. Nonradioactive airborne emissions from the 384 Powerhouse remained far below regulatory limits. The only other potential source of significant nonradioactive airborne emissions are the defense reactor fuel fabrication facilities located in the 300 Area. However, these facilities were shut down during calendar year (CY) 1987 and, therefore, were not a significant emission source. The fuel fabrication facilities are currently being placed in cold standby. There are a number of nonradioactive liquid effluent streams located in the 300 and 400 Areas. Of these, only the 300 Area process sewer system has a significant potential for the release of chemical contaminants to the environment. The other liquid effluent streams (e.g., the sanitary waste systems or the water treatment plant filter backwash) are single purpose, adequately characterized, and not significantly threatened by chemical contaminants. 3 refs., 7 tabs.

  5. Thermal quenching of thermoluminescence in TLD-200, TLD-300 and TLD-400 after β-irradiation

    NASA Astrophysics Data System (ADS)

    Kafadar, Vural E.

    2011-02-01

    The dosimetric characteristics of many TL materials are influenced by changes in location, size and shape of the glow curves due to changes in the heating rate. In this study, the effect of heating rate on the integrated peak areas of CaF 2:Dy (TLD-200), CaF 2:Tm (TLD-300) and CaF 2:Mn (TLD-400) crystals have been investigated after β-irradiation. It was observed that the peak temperatures of all peaks shifted to the high temperature sides and the integrated peak areas decrease as the heating rate increases due to thermal quenching, whose efficiency increases as temperature increases.

  6. 77 FR 26663 - Airworthiness Directives; The Boeing Company Model 767-200, -300, -300F, and -400ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-07

    ...We are superseding an existing airworthiness directive (AD) that applies to The Boeing Company Model 767-200, -300, and -300F series airplanes. That AD currently requires inspections to detect cracking or corrosion of the fail-safe straps between the side fitting of the rear spar bulkhead at body station 955 and the skin; and follow- on and corrective actions. This new AD expands the......

  7. Evaluations of University of Wisconsin Silicon Carbide Temperature Monitors 300 LO and 400 LO B

    SciTech Connect

    K. L. Davis; J. L. Rempe; D. L. Knudson; B. M. Chase; T. C. Unruh

    2011-12-01

    Silicon carbide (SiC) temperature monitors 05R4-02-A KG1403 (300 LO) and 05R4-01-A KG1415 (400 LO B) were evaluated at the High Temperature Test Lab (HTTL) to determine their peak irradiation temperatures. HTTL measurements indicate that the peak irradiation temperature for the 300 LO monitor was 295 {+-} 20 C and the peak irradiation temperature for the 400 LO B monitor was 294 {+-} 25 C. Two silicon carbide (SiC) temperature monitors irradiated in the Advanced Test Reactor (ATR) were evaluated at the High Temperature Test Lab (HTTL) to determine their peak temperature during irradiation. These monitors were irradiated as part of the University of Wisconsin Pilot Project with a target dose of 3 dpa. Temperature monitors were fabricated from high density (3.203 g/cm3) SiC manufactured by Rohm Haas with a nominal size of 12.5 mm x 1.0 mm x 0.75 mm (see Attachment A). Table 1 provides identification for each monitor with an expected peak irradiation temperature range based on preliminary thermal analysis (see Attachment B). Post irradiation calculations are planned to reduce uncertainties in these calculated temperatures. Since the early 1960s, SiC has been used as a post-irradiation temperature monitor. As noted in Reference 2, several researchers have observed that neutron irradiation induced lattice expansion of SiC annealed out when the post-irradiation annealing temperature exceeds the peak irradiation temperature. As noted in Reference 3, INL uses resistivity measurements to infer peak irradiation temperature from SiC monitors. Figure 1 depicts the equipment at the HTTL used to evaluate the SiC monitors. The SiC monitors are heated in the annealing furnace using isochronal temperature steps that, depending on customer needs, can range from 50 to 800 C. This furnace is located under a ventilation hood within the stainless steel enclosure. The ventilation system is activated during heating so that any released vapors are vented through this system. Annealing

  8. 75 FR 17882 - Airworthiness Directives; The Boeing Company Model 737-300, -400, and -500 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-08

    ... 2009-23-10, Amendment 39-16084 (74 FR 57564, November 9, 2009), for all Model 737-300, -400, and -500... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3... Amendment 39-16084 (74 FR 57564, November 9, 2009) and adding the following new AD: The Boeing...

  9. 76 FR 42607 - Airworthiness Directives; The Boeing Company Model 767-200, -300, and -400ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-19

    ...-15818 (74 FR 8717, February 26, 2009), for certain Model 767-200, -300, and - 400ER series airplanes...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979), (3) Will not affect... FR 8717, February 26, 2009), and adding the following new AD: The Boeing Company: Docket No....

  10. 76 FR 67343 - Airworthiness Directives; The Boeing Company Model 737-300, -400, and -500 Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-01

    ...-06 R1, Amendment 39-16015 (74 FR 45979, September 8, 2009), for certain Model 737-300, -400, and -500... AD 2009-02-06 R1, Amendment 39-16015 (74 FR 45979, September 8, 2009), we received a report of four... Information AD 2009-02-06 R1, Amendment 39-16015 (74 FR 45979, September 8, 2009), referred to Boeing...

  11. Effect of Dissolved Oxygen on the Filterability of Jet Fuels for Temperatures Between 300 Degrees and 400 Degrees F

    NASA Technical Reports Server (NTRS)

    Mckeown, Anderson B; Hibbard, Robert R

    1955-01-01

    The effect of dissolved oxygen in the filter-clogging characteristics of three JP-4 and two JP-5 fuels was studied at 300 degrees to 400 degrees F in a bench- scale rig, employing filter paper as the filter medium. The residence time of the fuel at the high temperature was approximately 6 seconds. For these conditions, the clogging characteristics of the fuels increased with both increasing temperature and increasing concentration of dissolved oxygen. The amount of insoluble material formed at high temperatures necessary to produce clogging of filters was very small, of the order of 1 milligram per gallon of fuel.

  12. DYNAMIC LOADING OF TEFLON AT 200?C

    SciTech Connect

    Urtiew, P A; Forbes, J W; Tarver, C M; Vandersall, K S; Garcia, F

    2007-06-13

    Dynamic loading experiments were performed on inert Teflon (Polytetrafluoroethylene) samples, initially heated to the temperature of 200 C, to test its behavior under these conditions for its use in other heated experiments. Tests were performed in the 100 mm diameter bore propellant driven gas gun with piezo-resistive manganin pressure gauges imbedded into the samples to measure loading pressures. Experimental data provided new information on the shock velocity - particle velocity relationship for the heated material and showed no adverse effect of temperature on the insulating properties of the material.

  13. Phenotypes and Virulence among Staphylococcus aureus USA100, USA200, USA300, USA400, and USA600 Clonal Lineages

    PubMed Central

    King, Jessica M.; Kulhankova, Katarina; Stach, Christopher S.; Vu, Bao G.

    2016-01-01

    ABSTRACT Staphylococcus aureus diseases affect ~500,000 individuals per year in the United States. Worldwide, the USA100, USA200, USA400, and USA600 lineages cause many of the life-threatening S. aureus infections, such as bacteremia, infective endocarditis, pneumonia, toxic shock syndrome, and surgical site infections. However, the virulence mechanisms associated with these clonal lineages, in particular the USA100 and USA600 isolates, have been severely understudied. We investigated the virulence of these strains, in addition to strains in the USA200, USA300, and USA400 types, in well-established in vitro assays and in vivo in the rabbit model of infective endocarditis and sepsis. We show in the infective endocarditis and sepsis model that strains in the USA100 and USA600 lineages cause high lethality and are proficient in causing native valve infective endocarditis. Strains with high cytolytic activity or producing toxic shock syndrome toxin 1 (TSST-1) or staphylococcal enterotoxin C (SEC) caused lethal sepsis, even with low cytolytic activity. Strains in the USA100, USA200, USA400, and USA600 lineages consistently contained genes that encode for the enterotoxin gene cluster proteins, SEC, or TSST-1 and were proficient at causing infective endocarditis, while the USA300 strains lacked these toxins and were deficient in promoting vegetation growth. The USA100, USA200, and USA400 strains in our collection formed strong biofilms in vitro, whereas the USA200 and USA600 strains exhibited increased blood survival. Hence, infective endocarditis and lethal sepsis are multifactorial and not intrinsic to any one individual clonal group, further highlighting the importance of expanding our knowledge of S. aureus pathogenesis to clonal lineages causative of invasive disease. IMPORTANCE S. aureus is the leading cause of infective endocarditis in the developed world, affecting ~40,000 individuals each year in the United States, and the second leading cause of bacteremia (D

  14. Phenotypes and Virulence among Staphylococcus aureus USA100, USA200, USA300, USA400, and USA600 Clonal Lineages.

    PubMed

    King, Jessica M; Kulhankova, Katarina; Stach, Christopher S; Vu, Bao G; Salgado-Pabón, Wilmara

    2016-01-01

    Staphylococcus aureus diseases affect ~500,000 individuals per year in the United States. Worldwide, the USA100, USA200, USA400, and USA600 lineages cause many of the life-threatening S. aureus infections, such as bacteremia, infective endocarditis, pneumonia, toxic shock syndrome, and surgical site infections. However, the virulence mechanisms associated with these clonal lineages, in particular the USA100 and USA600 isolates, have been severely understudied. We investigated the virulence of these strains, in addition to strains in the USA200, USA300, and USA400 types, in well-established in vitro assays and in vivo in the rabbit model of infective endocarditis and sepsis. We show in the infective endocarditis and sepsis model that strains in the USA100 and USA600 lineages cause high lethality and are proficient in causing native valve infective endocarditis. Strains with high cytolytic activity or producing toxic shock syndrome toxin 1 (TSST-1) or staphylococcal enterotoxin C (SEC) caused lethal sepsis, even with low cytolytic activity. Strains in the USA100, USA200, USA400, and USA600 lineages consistently contained genes that encode for the enterotoxin gene cluster proteins, SEC, or TSST-1 and were proficient at causing infective endocarditis, while the USA300 strains lacked these toxins and were deficient in promoting vegetation growth. The USA100, USA200, and USA400 strains in our collection formed strong biofilms in vitro, whereas the USA200 and USA600 strains exhibited increased blood survival. Hence, infective endocarditis and lethal sepsis are multifactorial and not intrinsic to any one individual clonal group, further highlighting the importance of expanding our knowledge of S. aureus pathogenesis to clonal lineages causative of invasive disease. IMPORTANCE S. aureus is the leading cause of infective endocarditis in the developed world, affecting ~40,000 individuals each year in the United States, and the second leading cause of bacteremia (D. R

  15. Background UV in the 300 to 400 nm region affecting the extended range detection of radioactive material

    NASA Astrophysics Data System (ADS)

    West, William Carey

    The desire to find alternative methods for the detection of radioactive material at extended ranges has resulted in an increased interest in the detection of the air fluorescence resulting from the alpha or beta radioactive particle's interaction with molecules of air. Air fluorescence photons travel further than the radioactive particles, allowing for detections at longer distances. However, any detection of the ultraviolet (UV) air fluorescence is dependant on overcoming natural and man-made background UV to achieve favorable signal to noise ratios. This research describes laboratory and field experiments conducted to determine the background UV in the 300 to 400 nm region of the electromagnetic spectrum for certain detection scenarios, and number of UV air fluorescence photons required to achieve detections with a certain confidence limit. The reflective, scintillation, and transmissive UV characteristics of some common materials are discussed and their contribution to a successful detection explored. Additionally, the contributions to the UV background from natural and man-made light sources are investigated. The successful outside optical detection of alpha and beta radioactive isotopes in the 300 to 400 nm region is possible in the lower part of the spectral region (i.e., near 316 nm), when there is no UV light from man-made sources in that band and only natural light exists. Alpha sources (i.e., 241Am) equal to or larger than 1.017 curies, theoretically can be detected with 95% confidence during nighttime scenarios with moonless overcast skies at a distances of 20 meters at 316 nm with the optical system assumed for these calculations. Additionally, where scintillators are available that can be employed near 90Sr radioactive sources, the detectable activities can be reduced by factors as high as 250. This allows for detections of sources in the millicuries. Tests results are presented for several common materials (e.g., polypropylene, high density

  16. 76 FR 36011 - Airworthiness Directives; Fokker Services B.V. Model F.27 Mark 050, 200, 300, 400, 500, 600, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-21

    ...'' (66 FR 23086, May 7, 2001). In addition to new airworthiness standards for transport airplanes and new... rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will.... Model F.27 Mark 050, 200, 300, 400, 500, 600, and 700 Airplanes; and Model F.28 Airplanes...

  17. TAE+ 5.1 - TRANSPORTABLE APPLICATIONS ENVIRONMENT PLUS, VERSION 5.1 (HP9000 SERIES 300/400 VERSION)

    NASA Technical Reports Server (NTRS)

    TAE SUPPORT OFFICE

    1994-01-01

    programs to display and control the user interfaces. Since the WPTs access the workbench-generated resource files during each execution, details such as color, font, location, and object type remain independent from the application code, allowing changes to the user interface without recompiling and relinking. In addition to WPTs, TAE Plus can control interaction of objects from the interpreted TAE Command Language. TCL provides a means for the more experienced developer to quickly prototype an application's use of TAE Plus interaction objects and add programming logic without the overhead of compiling or linking. TAE Plus requires MIT's X Window System, Version 11 Release 4, and the Open Software Foundation's Motif. The Workbench and WPTs are written in C++ and the remaining code is written in C. TAE Plus is available by license for an unlimited time period. The licensed program product includes the TAE Plus source code and one set of supporting documentation. Additional documentation may be purchased separately at the price indicated below. The amount of disk space required to load the TAE Plus tar format tape is between 35Mb and 67Mb depending on the machine version. The recommended minimum memory is 12Mb. Each TAE Plus platform delivery tape includes pre-built libraries and executable binary code for that particular machine, as well as source code, so users do not have to do an installation. Users wishing to recompile the source will need both a C compiler and either GNU's C++ Version 1.39 or later, or a C++ compiler based on AT&T 2.0 cfront. TAE Plus was developed in 1989 and version 5.2 was released in 1993. TAE Plus 5.2 is expected to be available on media suitable for seven different machine platforms: 1) DEC VAX computers running VMS (TK50 cartridge in VAX BACKUP format), 2) IBM RS/6000 series workstations running AIX (.25 inch tape cartridge in UNIX tar format), 3) DEC RISC workstations running ULTRIX (TK50 cartridge in UNIX tar format), 4) HP9000 Series 300/400

  18. TAE+ 5.1 - TRANSPORTABLE APPLICATIONS ENVIRONMENT PLUS, VERSION 5.1 (HP9000 SERIES 300/400 VERSION)

    NASA Technical Reports Server (NTRS)

    TAE SUPPORT OFFICE

    1994-01-01

    programs to display and control the user interfaces. Since the WPTs access the workbench-generated resource files during each execution, details such as color, font, location, and object type remain independent from the application code, allowing changes to the user interface without recompiling and relinking. In addition to WPTs, TAE Plus can control interaction of objects from the interpreted TAE Command Language. TCL provides a means for the more experienced developer to quickly prototype an application's use of TAE Plus interaction objects and add programming logic without the overhead of compiling or linking. TAE Plus requires MIT's X Window System, Version 11 Release 4, and the Open Software Foundation's Motif. The Workbench and WPTs are written in C++ and the remaining code is written in C. TAE Plus is available by license for an unlimited time period. The licensed program product includes the TAE Plus source code and one set of supporting documentation. Additional documentation may be purchased separately at the price indicated below. The amount of disk space required to load the TAE Plus tar format tape is between 35Mb and 67Mb depending on the machine version. The recommended minimum memory is 12Mb. Each TAE Plus platform delivery tape includes pre-built libraries and executable binary code for that particular machine, as well as source code, so users do not have to do an installation. Users wishing to recompile the source will need both a C compiler and either GNU's C++ Version 1.39 or later, or a C++ compiler based on AT&T 2.0 cfront. TAE Plus was developed in 1989 and version 5.2 was released in 1993. TAE Plus 5.2 is expected to be available on media suitable for seven different machine platforms: 1) DEC VAX computers running VMS (TK50 cartridge in VAX BACKUP format), 2) IBM RS/6000 series workstations running AIX (.25 inch tape cartridge in UNIX tar format), 3) DEC RISC workstations running ULTRIX (TK50 cartridge in UNIX tar format), 4) HP9000 Series 300/400

  19. Complex permittivity of lanthanum aluminate in the 20 to 300 K temperature range from 26.5 to 40.0 GHz

    NASA Technical Reports Server (NTRS)

    Miranda, F. A.; Gordon, W. L.; Bhasin, K. B.; Ebihara, B. T.; Heinen, V. O.; Chorey, C. M.

    1990-01-01

    Dielectric constants of microwave substrates are required in the design of superconducting microwave circuits at various temperatures. In this paper, the results are reported of a study of the complex permittivity of the newly developed lanthanum aluminate (LaAlO3) substrate, in the 20 to 300 K temperature range at frequencies from 26.5 to 40.0 GHz. The value of the complex permittivity was obtained by measuring the sample scattering parameters using a microwave waveguide technique. It is observed that, while the dielectric constant did not change appreciably with frequency, its value decreased by approximately 14 percent from room temperature to 20 K.

  20. Operational characteristics of a 200 C LC parallel resonant circuit

    NASA Technical Reports Server (NTRS)

    Baumann, Eric D.; Hammoud, Ahmad N.

    1995-01-01

    Research efforts are currently underway at the NASA Lewis Research Center to design and demonstrate an inverter capable of operating with a baseplate temperature of 200 C. In support of this project, various electrical components including capacitors, inductors, transformers, cables, and semiconductor switches are being developed or evaluated for integration into the inverter. In this work, a parallel LC resonant circuit was constructed and evaluated under simultaneous electrical and thermal stressing. The tests were performed in the temperature range of 25 to 200 C with an applied voltage of up to 90 V, 20 kHz. The individual components were comprised of high temperature film capacitors and powder core inductors developed in-house. The circuit was characterized in terms of the component currents and case temperatures as well as frequency of resonance as a function of applied bias and temperature. The results obtained, which have indicated good functional stability up to 200 C, are presented and discussed.

  1. Switching Characteristics of a 4H-SiC Based Bipolar Junction Transistor to 200 C

    NASA Technical Reports Server (NTRS)

    Niedra, Janis M.

    2006-01-01

    Static curves and resistive load switching characteristics of a 600 V, 4 A rated, SiC-based NPN bipolar power transistor (BJT) were observed at selected temperatures from room to 200 C. All testing was done in a pulse mode at low duty cycle (approx.0.1 percent). Turn-on was driven by an adjustable base current pulse and turn-off was accelerated by a negative base voltage pulse of 7 V. These base drive signals were implemented by 850 V, gated power pulsers, having rise-times of roughly 10 ns, or less. Base charge sweep-out with a 7 V negative pulse did not produce the large reverse base current pulse seen in a comparably rated Si-based BJT. This may be due to a very low charge storage time. The decay of the collector current was more linear than its exponential-like rise. Switching observations were done at base drive currents (I(sub B)) up to 400 mA and collector currents (I(sub C)) up to 4 A, using a 100 Omega non-inductive load. At I(sub B) = 400 mA and I(sub C) = 4 A, turn-on times typically varied from 80 to 94 ns, over temperatures from 23 to 200 C. As expected, lowering the base drive greatly extended the turn-on time. Similarly, decreasing the load current to I(sub C) = 1 A with I(sub B) = 400 mA produced turn-on times as short as 34 ns. Over the 23 to 200 C range, with I(sub B) = 400 mA and I(sub C) = 4 A, turn-off times were in the range of 72 to 84 ns with the 7 V sweep-out.

  2. Static and Switching Characteristics of a 4H-SiC Based BJT to 200 C

    NASA Technical Reports Server (NTRS)

    Niedra, Janis M.; Schwarze, Gene E.

    2006-01-01

    Static curves and resistive load switching characteristics of a 600 V, 4 A rated, SiC-based NPN bipolar power transistor (BJT) were observed at selected temperatures from 23 to 200 C. All testing was done in a pulse mode at low duty cycle (approx. 0.1 percent). Turn-on was driven by an adjustable base current pulse and turn-off was accelerated by a negative base voltage pulse of either 7 or 0.6 V. Switching observations were done at base drive currents (I(sub B)) up to 400 mA and collector currents (I(sub C)) up to 4 A, using a 100 OMEGA non-inductive load. At I(sub B) = 400 mA and I(sub C) = 4 A, turn-on times typically varied from 81 to 97 ns, over temperatures from 23 to 200 C. As expected, lowering the base drive greatly extended the turn-on time. Similarly, decreasing the load current to I(sub C) = 1 A with I(sub B) = 400 mA produced turn-on times as short as 30 ns. Over the 23 to 200 C range, with I(sub B) = 400 mA and I(sub C) = 4 A, turn-off times were in the range of 61 to 77 ns with the 7 V sweep-out and 130 to 150 ns with the 0.6 V sweep-out. At a fixed temperature and I(sub C), the turn-off time decreased slightly with decreasing I(sub B), for I(sub B) sufficient to still provide full turn-on. The ratio of conduction to switching losses is estimated, based on the observed I(sub C) transition times and static curves. An estimate at 200 kHz and a 50 percent duty cycle shows that under practicable conditions the two losses can be comparable. Hence the evidence obtained does not support the occasionally voiced concern of necessarily unacceptably high conduction losses in SiC-based BJTs.

  3. The Behavior of IAPWS-95 from 250 to 300 K and Pressures up to 400 MPa: Evaluation Based on Recently Derived Property Data

    SciTech Connect

    Wagner, Wolfgang Thol, Monika

    2015-12-15

    Over the past several years, considerable scientific and technical interest has been focused on accurate thermodynamic properties of fluid water covering part of the subcooled (metastable) region and the stable liquid from the melting line up to about 300 K and pressures up to several hundred MPa. Between 2000 and 2010, experimental density data were published whose accuracy was not completely clear. The scientific standard equation of state for fluid water, the IAPWS-95 formulation, was developed on the basis of experimental data for thermodynamic properties that were available by 1995. In this work, it is examined how IAPWS-95 behaves with respect to the experimental data published after 1995. This investigation is carried out for temperatures from 250 to 300 K and pressures up to 400 MPa. The starting point is the assessment of the current data situation. This was mainly performed on the basis of data for the density, expansivity, compressibility, and isobaric heat capacity, which were derived in 2015 from very accurate speed-of-sound data. Apart from experimental data and these derived data, property values calculated from the recently published equation of state for this region of Holten et al. (2014) were also used. As a result, the unclear data situation could be clarified, and uncertainty values could be estimated for the investigated properties. In the region described above, detailed comparisons show that IAPWS-95 is able to represent the latest experimental data for the density, expansivity, compressibility, speed of sound, and isobaric heat capacity to within the uncertainties given in the release on IAPWS-95. Since the release does not contain uncertainty estimates for expansivities and compressibilities, the statement relates to the error propagation of the given uncertainty in density. Due to the lack of experimental data for the isobaric heat capacity for pressures above 100 MPa, no uncertainty estimates are given in the release for this pressure

  4. The Behavior of IAPWS-95 from 250 to 300 K and Pressures up to 400 MPa: Evaluation Based on Recently Derived Property Data

    NASA Astrophysics Data System (ADS)

    Wagner, Wolfgang; Thol, Monika

    2015-12-01

    Over the past several years, considerable scientific and technical interest has been focused on accurate thermodynamic properties of fluid water covering part of the subcooled (metastable) region and the stable liquid from the melting line up to about 300 K and pressures up to several hundred MPa. Between 2000 and 2010, experimental density data were published whose accuracy was not completely clear. The scientific standard equation of state for fluid water, the IAPWS-95 formulation, was developed on the basis of experimental data for thermodynamic properties that were available by 1995. In this work, it is examined how IAPWS-95 behaves with respect to the experimental data published after 1995. This investigation is carried out for temperatures from 250 to 300 K and pressures up to 400 MPa. The starting point is the assessment of the current data situation. This was mainly performed on the basis of data for the density, expansivity, compressibility, and isobaric heat capacity, which were derived in 2015 from very accurate speed-of-sound data. Apart from experimental data and these derived data, property values calculated from the recently published equation of state for this region of Holten et al. (2014) were also used. As a result, the unclear data situation could be clarified, and uncertainty values could be estimated for the investigated properties. In the region described above, detailed comparisons show that IAPWS-95 is able to represent the latest experimental data for the density, expansivity, compressibility, speed of sound, and isobaric heat capacity to within the uncertainties given in the release on IAPWS-95. Since the release does not contain uncertainty estimates for expansivities and compressibilities, the statement relates to the error propagation of the given uncertainty in density. Due to the lack of experimental data for the isobaric heat capacity for pressures above 100 MPa, no uncertainty estimates are given in the release for this pressure

  5. Measurements of complex permittivity of microwave substrates in the 20 to 300 K temperature range from 26.5 to 40.0 GHz

    NASA Technical Reports Server (NTRS)

    Miranda, Felix A.; Gordon, William L.; Heinen, Vernon O.; Ebihara, Ben T.; Bhasin, Kul B.

    1989-01-01

    A knowledge of the dielectric properties of microwave substrates at low temperatures is useful in the design of superconducting microwave circuits. Results are reported for a study of the complex permittivity of sapphire (Al2O3), magnesium oxide (MgO), silicon oxide (SiO2), lanthanum aluminate (LaAlO3), and zirconium oxide (ZrO2), in the 20 to 300 Kelvin temperature range, at frequencies from 26.5 to 40.0 GHz. The values of the real and imaginary parts of the complex permittivity were obtained from the scattering parameters, which were measured using a HP-8510 automatic network analyzer. For these measurements, the samples were mounted on the cold head of a helium gas closed cycle refrigerator, in a specially designed vacuum chamber. An arrangement of wave guides, with mica windows, was used to connect the cooling system to the network analyzer. A decrease in the value of the real part of the complex permittivity of these substrates, with decreasing temperature, was observed. For MgO and Al2O3, the decrease from room temperature to 20 K was of 7 and 15 percent, respectively. For LaAlO3, it decreased by 14 percent, for ZrO2 by 15 percent, and for SiO2 by 2 percent, in the above mentioned temperature range.

  6. Measurements of complex permittivity of microwave substrates in the 20 to 300 K temperature range from 26.5 to 40.0 GHz

    NASA Technical Reports Server (NTRS)

    Miranda, Felix A.; Gordon, William L.; Heinen, Vernon O.; Ebihara, Ben T.; Bhasin, Kul B.

    1990-01-01

    A knowledge of the dielectric properties of microwve substrates at low temperatures is useful in the design of superconducting microwave circuits. Results are reported for a study of the complex permittivity of sapphire (Al2O3), magnesium oxide (MgO), silicon oxide (SiO2), lanthanum aluminate (LaAlO3), and zirconium oxide (ZrO2), in the 20 to 300 Kelvin temperature range, at frequencies from 26.5 to 40.0 GHz. The values of the real and imaginary parts of the complex permittivity were obtained from the scattering parameters, which were measured using an HP-8510 automatic network analyzer. For these measurements, the samples were mounted on the cold head of a helium gas closed cycle refrigerator, in a specially designated vacuum chamber. An arrangement of wave guides, with mica windows, was used to connect the cooling system to the network analyzer. A decrease in the value of the real part of the complex permittivity of these substrates, with decreasing temperature, was observed. For MgO and Al2O3, the decrease from room temperature to 20 K was of 7 and 15 percent, respectively. For LaAlO3, it decreased by 14 percent, for ZrO2 by 15 percent, and for SiO2 by 2 percent, in the above mentioned temperature range.

  7. miR-200c Contributes to Injury From Transient Focal Cerebral Ischemia by Targeting Reelin

    PubMed Central

    Stary, Creed M.; Xu, Lijun; Sun, Xiaoyun; Ouyang, Yi-Bing; White, Robin E.; Leong, Jason; Li, John; Xiong, Xiaoxing; Giffard, Rona G.

    2015-01-01

    Background and Purpose miR-200c increases rapidly in the brain following transient cerebral ischemia but its role in post-stroke brain injury is unclear. Reelin, a regulator of neuronal migration and synaptogenesis, is a predicted target of miR-200c. We hypothesized that miR-200c contributes to injury from transient cerebral ischemia by targeting reelin. Methods Brain infarct volume, neurological score and levels of miR-200c, reelin mRNA and reelin protein were assessed in mice subjected to 1 hr of middle cerebral artery occlusion (MCAO) with/without intracerebroventricular infusion of miR-200c antagomir, mimic or mismatch control. Direct targeting of reelin by miR-200c was assessed in vitro by dual luciferase assay and immunoblot. Results Pre-treatment with miR-200c antagomir decreased post-MCAO brain levels of miR-200c, resulting in a significant reduction in infarct volume and neurologic deficit. Changes in brain levels of miR-200c inversely correlated with reelin protein expression. Direct targeting of the Reln 3’UTR by miR-200c was verified with dual luciferase assay. Inhibition of miR-200c resulted in an increase in cell survival subsequent to in vitro oxidative injury. This effect was blocked by knockdown of reelin mRNA, while application of reelin protein afforded protection. Conclusions These findings suggest that the post-stroke increase in miR-200c contributes to brain cell death by inhibiting reelin expression, and that reducing post-stroke miR-200c is a potential target to mitigate stroke induced brain injury. PMID:25604249

  8. Safety and efficacy of switching to nilotinib 400 mg twice daily for patients with chronic myeloid leukemia in chronic phase with suboptimal response or failure on front-line imatinib or nilotinib 300 mg twice daily

    PubMed Central

    Hughes, Timothy P.; Hochhaus, Andreas; Kantarjian, Hagop M.; Cervantes, Francisco; Guilhot, François; Niederwieser, Dietger; le Coutre, Philipp D.; Rosti, Gianantonio; Ossenkoppele, Gert; Lobo, Clarisse; Shibayama, Hirohiko; Fan, Xiaolin; Menssen, Hans D.; Kemp, Charisse; Larson, Richard A.; Saglio, Giuseppe

    2014-01-01

    In a randomized, phase III trial of nilotinib versus imatinib in patients with newly diagnosed Philadelphia chromosome positive chronic myeloid leukemia in chronic phase, more patients had suboptimal response or treatment failure on front-line imatinib than on nilotinib. Patients with suboptimal response/treatment failure on imatinib 400 mg once or twice daily or nilotinib 300 mg twice daily could enter an extension study to receive nilotinib 400 mg twice daily. After a 19-month median follow up, the safety profile of nilotinib 400 mg twice daily in patients switching from imatinib (n=35) was consistent with previous reports, and few new adverse events occurred in patients escalating from nilotinib 300 mg twice daily (n=19). Of patients previously treated with imatinib or nilotinib 300 mg twice daily, respectively, 15 of 26 (58%) and 2 of 6 (33%) without complete cytogenetic response at extension study entry, and 11 of 34 (32%) and 7 of 18 (39%) without major molecular response at extension study entry, achieved these responses at any time on nilotinib 400 mg twice daily. Estimated 18-month rates of freedom from progression and overall survival after entering the extension study were lower for patients switched from imatinib (85% and 87%, respectively) versus nilotinib 300 mg twice daily (95% and 94%, respectively). Nilotinib dose escalation was generally well tolerated and improved responses in about one-third of patients with suboptimal response/treatment failure. Switch to nilotinib improved responses in some patients with suboptimal response/treatment failure on imatinib, but many did not achieve complete cytogenetic response (clinicaltrials.gov identifiers:00718263, 00471497 - extension). PMID:24532039

  9. 75 FR 13046 - Airworthiness Directives; The Boeing Company Model 747-200C, -200F, -400, -400D, and -400F Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-18

    ... February 27, 2006, we issued AD 2006-05-09, amendment 39-14506 (71 FR 12122, March 9, 2006), for certain... resulted from fatigue tests and an analysis that identified areas of the fuselage lap joints where fatigue... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR...

  10. miR-200c dampens cancer cell migration via regulation of protein kinase A subunits.

    PubMed

    Sigloch, Florian Christoph; Burk, Ulrike Christina; Biniossek, Martin Lothar; Brabletz, Thomas; Schilling, Oliver

    2015-09-15

    Expression of miR-200c is a molecular switch to determine cellular fate towards a mesenchymal or epithelial phenotype. miR-200c suppresses the early steps of tumor progression by preventing epithelial-mesenchymal transition (EMT) and intravasation of tumor cells. Unraveling the underlying molecular mechanisms might pinpoint to novel therapeutic options. To better understand these mechanisms it is crucial to identify targets of miR-200c. Here, we employ a combination of quantitative proteomic and bioinformatic strategies to identify novel miR-200c targets. We identify and confirm two subunits of the central cellular kinase protein kinase A (PKA), namely PRKAR1A and PRKACB, to be directly regulated by miR-200c. Notably, siRNA-mediated downregulation of both proteins phenocopies the migratory behavior of breast cancer cells after miR-200c overexpression. Patient data from publicly accessible databases supports a miR-200c-PKA axis. Thus, our study identifies the PKA heteroprotein as an important mediator of miR-200c induced repression of migration in breast cancer cells. By bioinformatics, we define a miRNA target cluster consisting of PRKAR1A, PRKAR2B, PRKACB, and COF2, which is targeted by a group of 14 miRNAs. PMID:26203557

  11. miR-200c dampens cancer cell migration via regulation of protein kinase a subunits

    PubMed Central

    Biniossek, Martin Lothar; Brabletz, Thomas; Schilling, Oliver

    2015-01-01

    Expression of miR-200c is a molecular switch to determine cellular fate towards a mesenchymal or epithelial phenotype. miR-200c suppresses the early steps of tumor progression by preventing epithelial-mesenchymal transition (EMT) and intravasation of tumor cells. Unraveling the underlying molecular mechanisms might pinpoint to novel therapeutic options. To better understand these mechanisms it is crucial to identify targets of miR-200c. Here, we employ a combination of quantitative proteomic and bioinformatic strategies to identify novel miR-200c targets. We identify and confirm two subunits of the central cellular kinase protein kinase A (PKA), namely PRKAR1A and PRKACB, to be directly regulated by miR-200c. Notably, siRNA-mediated downregulation of both proteins phenocopies the migratory behavior of breast cancer cells after miR-200c overexpression. Patient data from publicly accessible databases supports a miR-200c-PKA axis. Thus, our study identifies the PKA heteroprotein as an important mediator of miR-200c induced repression of migration in breast cancer cells. By bioinformatics, we define a miRNA target cluster consisting of PRKAR1A, PRKAR2B, PRKACB, and COF2, which is targeted by a group of 14 miRNAs. PMID:26203557

  12. MiR-200c promotes bladder cancer cell migration and invasion by directly targeting RECK

    PubMed Central

    Cheng, Yidong; Zhang, Xiaolei; Li, Peng; Yang, Chengdi; Tang, Jinyuan; Deng, Xiaheng; Yang, Xiao; Tao, Jun; Lu, Qiang; Li, Pengchao

    2016-01-01

    Background Increasing evidence suggests that the dysregulation of certain microRNAs plays an important role in tumorigenesis and metastasis. MiR-200c exhibits a disordered expression in many tumors and presents dual roles in bladder cancer (BC). Therefore, the definite role of miR-200c in BC needs to be investigated further. Materials and methods Quantitative reverse transcription polymerase chain reaction was used to assess miR-200c expression. Cell invasion and migration were evaluated using wound healing and transwell assays. The luciferase reporter assay was used to identify the direct target of miR-200c. The expression of reversion-inducing cysteine-rich protein with kazal motifs (RECK) in BC tissues and adjacent nontumor tissues, as well as in BC cell lines, was detected through quantitative reverse transcription polymerase chain reaction, Western blot assay, and immunohistochemistry. Results The miR-200c expression was significantly upregulated in the BC tissues compared with the adjacent nontumor tissues. The downregulation of miR-200c significantly inhibited cell migration and invasion in the BC cell lines. The luciferase reporter assay showed that RECK was a direct target of miR-200c. The knockdown of RECK in the BC cell lines treated with anti-miR-200c elevated the previously attenuated cell migration and invasion. Conclusion Our findings indicated that miR-200c functions as oncogenes in BC and may provide a novel therapeutic strategy for the treatment of BC. PMID:27574450

  13. The electrolyte challenge for a direct methanol-air polymer electrolyte fuel cell operating at temperatures up to 200 C

    NASA Technical Reports Server (NTRS)

    Savinell, Robert; Yeager, Ernest; Tryk, Donald; Landau, Uziel; Wainright, Jesse; Gervasio, Dominic; Cahan, Boris; Litt, Morton; Rogers, Charles; Scherson, Daniel

    1993-01-01

    Novel polymer electrolytes are being evaluated for use in a direct methanol-air fuel cell operating at temperatures in excess of 100 C. The evaluation includes tests of thermal stability, ionic conductivity, and vapor transport characteristics. The preliminary results obtained to date indicate that a high temperature polymer electrolyte fuel cell is feasible. For example, Nafion 117 when equilibrated with phosphoric acid has a conductivity of at least 0.4 Omega(exp -1)cm(exp -1) at temperatures up to 200 C in the presence of 400 torr of water vapor and methanol vapor cross over equivalent to 1 mA/cm(exp 2) under a one atmosphere methanol pressure differential at 135 C. Novel polymers are also showing similar encouraging results. The flexibility to modify and optimize the properties by custom synthesis of these novel polymers presents an exciting opportunity to develop an efficient and compact methanol fuel cell.

  14. Development of a 1 kW, 200 C Mapham Inventor

    NASA Technical Reports Server (NTRS)

    Hammoud, Ahmad; Gerber, Scott; Bauman, Eric; Overton, Eric; Myers, Ira; Bercaw, Robert

    1995-01-01

    Electronic systems and components are often exposed to high temperature environment in space-based applications, nuclear power facilities, and geothermal energy extraction fields. A key requirement for these systems is, therefore, to withstand the high temperature exposure while maintaining efficient and reliable operation. Efforts were taken to design and develop a high temperature power inverter capable of 200 C operation. A 1 kW, 20 kHz Mapham inverter was designed and evaluated as a function of temperature at different load levels. The inverter system, excluding its input, control, and logic circuits, was characterized at temperatures from ambient to 200 C at 0%, 50%, and 100% resistive loading. With an applied input voltage of 75 VDC, the inverter produced an output of 250 VAC. The results obtained, which indicate good operational characteristics of the inverter up to 200 C, are presented and discussed.

  15. miR-200c: a versatile watchdog in cancer progression, EMT, and drug resistance.

    PubMed

    Mutlu, Merve; Raza, Umar; Saatci, Özge; Eyüpoğlu, Erol; Yurdusev, Emre; Şahin, Özgür

    2016-06-01

    MicroRNAs (miRNAs) are 20-22-nucleotide small endogenous non-coding RNAs which regulate gene expression at post-transcriptional level. In the last two decades, identification of almost 2600 miRNAs in human and their potential to be modulated opened a new avenue to target almost all hallmarks of cancer. miRNAs have been classified as tumor suppressors or oncogenes depending on the phenotype they induce, the targets they modulate, and the tissue where they function. miR-200c, an illustrious tumor suppressor, is one of the highly studied miRNAs in terms of development, stemness, proliferation, epithelial-mesenchymal transition (EMT), therapy resistance, and metastasis. In this review, we first focus on the regulation of miR-200c expression and its role in regulating EMT in a ZEB1/E-cadherin axis-dependent and ZEB1/E-cadherin axis-independent manner. We then describe the role of miR-200c in therapy resistance in terms of multidrug resistance, chemoresistance, targeted therapy resistance, and radiotherapy resistance in various cancer types. We highlight the importance of miR-200c at the intersection of EMT and chemoresistance. Furthermore, we show how miR-200c coordinates several important signaling cascades such as TGF-β signaling, PI3K/Akt signaling, Notch signaling, VEGF signaling, and NF-κB signaling. Finally, we discuss miR-200c as a potential prognostic/diagnostic biomarker in several diseases, but mainly focusing on cancer and its potential application in future therapeutics. PMID:27094812

  16. 40 CFR 300.400 - General.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... decision-making stages; (7) Encourage the involvement and sharing of technology by industry and other... response; (4) Be sensitive to local community concerns; (5) Consider using treatment technologies; (6... industry. (d) Entry and access. (1) For purposes of determining the need for response, or choosing...

  17. 40 CFR 300.400 - General.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... COMMUNITY RIGHT-TO-KNOW PROGRAMS NATIONAL OIL AND HAZARDOUS SUBSTANCES POLLUTION CONTINGENCY PLAN Hazardous... pollutant or contaminant that may present an imminent and substantial danger to the public health or welfare... constitutes a public health or environmental emergency and no other person with the authority and...

  18. 40 CFR 300.400 - General.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... COMMUNITY RIGHT-TO-KNOW PROGRAMS NATIONAL OIL AND HAZARDOUS SUBSTANCES POLLUTION CONTINGENCY PLAN Hazardous... pollutant or contaminant that may present an imminent and substantial danger to the public health or welfare... constitutes a public health or environmental emergency and no other person with the authority and...

  19. 40 CFR 300.400 - General.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... industry. (d) Entry and access. (1) For purposes of determining the need for response, or choosing or... place or property where entry is necessary to determine the need for response or the appropriate... appropriate federal agency, that it is reasonable to believe that there may be or has been a release or...

  20. 40 CFR 300.400 - General.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... water supplies due to deterioration of the system through ordinary use. (c) Fund-financed action. In... actions, as described in subpart F of this part; (3) Conserve Fund monies by encouraging private...

  1. Inhibition of miR-200c Restores Endothelial Function in Diabetic Mice Through Suppression of COX-2.

    PubMed

    Zhang, Huina; Liu, Jian; Qu, Dan; Wang, Li; Luo, Jiang-Yun; Lau, Chi Wai; Liu, Pingsheng; Gao, Zhen; Tipoe, George L; Lee, Hung Kay; Ng, Chi Fai; Ma, Ronald Ching Wan; Yao, Xiaoqiang; Huang, Yu

    2016-05-01

    Endothelial dysfunction plays a crucial role in the development of diabetic vasculopathy. Our initial quantitative PCR results showed an increased miR-200c expression in arteries from diabetic mice and patients with diabetes. However, whether miR-200c is involved in diabetic endothelial dysfunction is unknown. Overexpression of miR-200c impaired endothelium-dependent relaxations (EDRs) in nondiabetic mouse aortas, whereas suppression of miR-200c by anti-miR-200c enhanced EDRs in diabetic db/db mice. miR-200c suppressed ZEB1 expression, and ZEB1 overexpression ameliorated endothelial dysfunction induced by miR-200c or associated with diabetes. More importantly, overexpression of anti-miR-200c or ZEB1 in vivo attenuated miR-200c expression and improved EDRs in db/db mice. Mechanistic study with the use of COX-2(-/-) mice revealed that COX-2 mediated miR-200c-induced endothelial dysfunction and that miR-200c upregulated COX-2 expression in endothelial cells through suppression of ZEB1 and increased production of prostaglandin E2, which also reduced EDR. This study demonstrates for the first time to our knowledge that miR-200c is a new mediator of diabetic endothelial dysfunction and inhibition of miR-200c rescues EDRs in diabetic mice. These new findings suggest the potential usefulness of miR-200c as the target for drug intervention against diabetic vascular complications. PMID:26822089

  2. Early antihepatitis C virus response with second-generation C200/C22 ELISA.

    PubMed

    van der Poel, C L; Bresters, D; Reesink, H W; Plaisier, A A; Schaasberg, W; Leentvaar-Kuypers, A; Choo, Q L; Quan, S; Polito, A; Houghton, M

    1992-01-01

    Detection of early antibody to hepatitis C virus (HCV) by a new second-generation C200/C22 anti-HCV enzyme-linked immunosorbent assay (ELISA) and a four-antigen recombinant immunoblot assay (4-RIBA) was compared with the first-generation anti-HCV C100 ELISA using sequential serum samples of 9 recipients who were infected with HCV, as detected by polymerase chain reaction after transfusion of blood products. Within 26 weeks after transfusion, 9/9 (100%) recipients seroconverted with C200/22 ELISA, and 6/9 (67%) seroconverted with C100 ELISA. Compared with C100 ELISA, C200/C22 ELISA seroconversion occurred simultaneously in 3 cases, 5-6 weeks earlier in 3 other cases, and 20 weeks earlier in 1 case. Seven of 9 (78%) recipients became positive, and 2/9 (22%) became indeterminate with 4-RIBA. In 8 cases with clinical posttransfusion hepatitis non-A, non-B (PTH-NANB), anti-HCV C200/C22 ELISA seroconversion occurred 2-17 (mean 6) weeks after the onset of hepatitis. In 6 cases of PTH-NANB, anti-HCV C100 ELISA seroconversion occurred 2-26 (mean 9) weeks after the onset of hepatitis. It is concluded that the second-generation C200/C22 ELISA is more sensitive than the C100 ELISA for the detection of antibody during early HCV infection. Indeterminate 4-RIBA results are found in the early phase of HCV infection. PMID:1322611

  3. Regulation of miR-200c by nuclear receptors PPAR{alpha}, LRH-1 and SHP

    SciTech Connect

    Zhang, Yuxia; Yang, Zhihong; Whitby, Richard; Wang, Li

    2011-12-09

    Highlights: Black-Right-Pointing-Pointer Knockdown of PPAR{alpha} and LRH-1 abolishes miR-200c inhibition of HCC cell migration. Black-Right-Pointing-Pointer SHP represses miR-200c expression via inhibition of the activity of PPAR{alpha} and LRH-1. Black-Right-Pointing-Pointer RJW100 exhibits strong ability to downregulate ZEB1 and ZEB2 proteins. -- Abstract: We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPAR{alpha} and LRH-1 siRNAs. The expression of miR-200c was decreased by PPAR{alpha}/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins. Co-expression of PPAR{alpha} and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter. This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors.

  4. Prognostic Role of MicroRNA-200c-141 Cluster in Various Human Solid Malignant Neoplasms

    PubMed Central

    Li, Xiao-yang; Li, Hui; Bu, Jie; Xiong, Liang; Guo, Hong-bin; Liu, Li-hong; Xiao, Tao

    2015-01-01

    The miR-200 family has emerged recently as a noticeable marker for predicting cancer prognosis and tumor progression. We aimed to review the evidence of miR-200c-141 genomic cluster as prognostic biomarkers in cancers. The results suggested that high level of miR-200c had no significant impact on OS (HR = 1.14 [0.77–1.69], P = 0.501) and DFS/PFS (HR = 0.72 [0.45–1.14], P = 0.161). Stratified analyses revealed that high miR-200c expression was significantly related to poor OS in serum/plasma (HR = 2.12 [1.62–2.77], P = 0.000) but not in tissues (HR = 0.89 [0.58–1.37], P = 0.599). High miR-200c expression was significantly associated with favorable DFS/PFS in tissues (HR = 0.56 [0.43–0.73], P = 0.000) but worse DFS/PFS in serum/plasma (HR = 1.90 [1.08–3.36], P = 0.027). For miR-141, we found that high miR-141 expression predicted no significant impact on OS (HR = 1.18 [0.74–1.88], P = 0.482) but poor DFS/PFS (HR = 1.11 [1.04–1.20], P = 0.003). Similarly, subgroup analyses showed that high miR-141 expression predicted poor OS in serum/plasma (HR = 4.34 [2.30–8.21], P = 0.000) but not in tissues (HR = 1.00 [0.92–1.09], P = 0.093). High miR-141 expression was significantly associated with worse DFS/PFS in tissues (HR = 1.12 [1.04–1.20], P = 0.002) but not in serum/plasma (HR = 0.90 [0.44–1.83], P = 0.771). Our findings indicated that, compared to their tissue counterparts, the expression level of miR-200c and miR-141 in peripheral blood may be more effective for monitoring cancer prognosis. High miR-141 expression was better at predicting tumor progression than survival for malignant tumors. PMID:26556949

  5. The Pitx2:miR-200c/141:noggin pathway regulates Bmp signaling and ameloblast differentiation

    PubMed Central

    Cao, Huojun; Jheon, Andrew; Li, Xiao; Sun, Zhao; Wang, Jianbo; Florez, Sergio; Zhang, Zichao; McManus, Michael T.; Klein, Ophir D.; Amendt, Brad A.

    2013-01-01

    The mouse incisor is a remarkable tooth that grows throughout the animal’s lifetime. This continuous renewal is fueled by adult epithelial stem cells that give rise to ameloblasts, which generate enamel, and little is known about the function of microRNAs in this process. Here, we describe the role of a novel Pitx2:miR-200c/141:noggin regulatory pathway in dental epithelial cell differentiation. miR-200c repressed noggin, an antagonist of Bmp signaling. Pitx2 expression caused an upregulation of miR-200c and chromatin immunoprecipitation assays revealed endogenous Pitx2 binding to the miR-200c/141 promoter. A positive-feedback loop was discovered between miR-200c and Bmp signaling. miR-200c/141 induced expression of E-cadherin and the dental epithelial cell differentiation marker amelogenin. In addition, miR-203 expression was activated by endogenous Pitx2 and targeted the Bmp antagonist Bmper to further regulate Bmp signaling. miR-200c/141 knockout mice showed defects in enamel formation, with decreased E-cadherin and amelogenin expression and increased noggin expression. Our in vivo and in vitro studies reveal a multistep transcriptional program involving the Pitx2:miR-200c/141:noggin regulatory pathway that is important in epithelial cell differentiation and tooth development. PMID:23863486

  6. miR-200c regulates FGFR-dependent epithelial proliferation via Vldlr during submandibular gland branching morphogenesis

    PubMed Central

    Rebustini, Ivan T.; Hayashi, Toru; Reynolds, Andrew D.; Dillard, Melvin L.; Carpenter, Ellen M.; Hoffman, Matthew P.

    2012-01-01

    The regulation of epithelial proliferation during organ morphogenesis is crucial for normal development, as dysregulation is associated with tumor formation. Non-coding microRNAs (miRNAs), such as miR-200c, are post-transcriptional regulators of genes involved in cancer. However, the role of miR-200c during normal development is unknown. We screened miRNAs expressed in the mouse developing submandibular gland (SMG) and found that miR-200c accumulates in the epithelial end buds. Using both loss- and gain-of-function, we demonstrated that miR-200c reduces epithelial proliferation during SMG morphogenesis. To identify the mechanism, we predicted miR-200c target genes and confirmed their expression during SMG development. We discovered that miR-200c targets the very low density lipoprotein receptor (Vldlr) and its ligand reelin, which unexpectedly regulate FGFR-dependent epithelial proliferation. Thus, we demonstrate that miR-200c influences FGFR-mediated epithelial proliferation during branching morphogenesis via a Vldlr-dependent mechanism. miR-200c and Vldlr may be novel targets for controlling epithelial morphogenesis during glandular repair or regeneration. PMID:22115756

  7. A 200 C Universal Gate Driver Integrated Circuit for Extreme Environment Applications

    SciTech Connect

    Tolbert, Leon M; Huque, Mohammad A; Islam, Syed K; Blalock, Benjamin J

    2012-01-01

    High-temperature power converters (dc-dc, dc-ac, etc.) have enormous potential in extreme environment applications, including automotive, aerospace, geothermal, nuclear, and well logging. For successful realization of such high-temperature power conversion modules, the associated control electronics also need to perform at high temperature. This paper presents a silicon-on-insulator (SOI) based high-temperature gate driver integrated circuit (IC) incorporating an on-chip low-power temperature sensor and demonstrating an improved peak output current drive over our previously reported work. This driver IC has been primarily designed for automotive applications, where the underhood temperature can reach 200 C. This new gate driver prototype has been designed and implemented in a 0.8 {micro}m, 2-poly, and 3-metal bipolar CMOS-DMOS (Double-Diffused Metal-Oxide Semiconductor) on SOI process and has been successfully tested for up to 200 C ambient temperature driving a SiC MOSFET and a SiC normally-ON JFET. The salient feature of the proposed universal gate driver is its ability to drive power switches over a wide range of gate turn-ON voltages such as MOSFET (0 to 20 V), normally-OFF JFET (-7 to 3 V), and normally-ON JFET (-20 to 0 V). The measured peak output current capability of the driver is around 5 A and is thus capable of driving several power switches connected in parallel. An ultralow-power on-chip temperature supervisory circuit has also been integrated into the die to safeguard the driver circuit against excessive die temperature ({ge}220 C). This approach utilizes increased diode leakage current at higher temperature to monitor the die temperature. The power consumption of the proposed temperature sensor circuit is below 10 {micro}W for operating temperature up to 200 C.

  8. MicroRNA-200c Represses IL-6, IL-8, and CCL-5 Expression and Enhances Osteogenic Differentiation

    PubMed Central

    Sharp, Thad; Khorsand, Behnoush; Fischer, Carol; Eliason, Steven; Salem, Ali; Akkouch, Adil; Brogden, Kim; Amendt, Brad A.

    2016-01-01

    MicroRNAs (miRs) regulate inflammation and BMP antagonists, thus they have potential uses as therapeutic reagents. However, the molecular function of miR-200c in modulating proinflammatory and bone metabolic mediators and osteogenic differentiation is not known. After miR-200c was transduced into a human embryonic palatal mesenchyme (HEPM) (a cell line of preosteoblasts), using lentiviral vectors, the resulting miR-200c overexpression increased osteogenic differentiation biomarkers, including osteocalcin (OCN) transcripts and calcium content. miR-200c expression also down-regulated interleukin (IL)-6, IL-8, and chemokine (C-C motif) ligand (CCL)-5 under lipopolysaccharide (LPS) stimulation and increased osteoprotegerin (OPG) in these cells. miR-200c directly regulates the expression of IL-6, IL-8 and CCL-5 transcripts by binding to their 3’UTRs. A plasmid-based miR-200c inhibitor effectively reduces their binding activities. Additionally, miR-200c delivered using polyethylenimine (PEI) nanoparticles effectively inhibits IL-6, IL-8 and CCL-5 in primary human periodontal ligament fibroblasts and increases the biomarkers of osteogenic differentiation in human bone marrow mesenchymal stem cells (MSCs), including calcium content, ALP, and Runx2. These data demonstrate that miR-200c represses IL-6, IL-8 and CCL-5 and improves osteogenic differentiation. miR-200c may potentially be used as an effective means to prevent periodontitis-associated bone loss by arresting inflammation and osteoclastogenesis and enhancing bone regeneration. PMID:27529418

  9. MicroRNA-200c Represses IL-6, IL-8, and CCL-5 Expression and Enhances Osteogenic Differentiation.

    PubMed

    Hong, Liu; Sharp, Thad; Khorsand, Behnoush; Fischer, Carol; Eliason, Steven; Salem, Ali; Akkouch, Adil; Brogden, Kim; Amendt, Brad A

    2016-01-01

    MicroRNAs (miRs) regulate inflammation and BMP antagonists, thus they have potential uses as therapeutic reagents. However, the molecular function of miR-200c in modulating proinflammatory and bone metabolic mediators and osteogenic differentiation is not known. After miR-200c was transduced into a human embryonic palatal mesenchyme (HEPM) (a cell line of preosteoblasts), using lentiviral vectors, the resulting miR-200c overexpression increased osteogenic differentiation biomarkers, including osteocalcin (OCN) transcripts and calcium content. miR-200c expression also down-regulated interleukin (IL)-6, IL-8, and chemokine (C-C motif) ligand (CCL)-5 under lipopolysaccharide (LPS) stimulation and increased osteoprotegerin (OPG) in these cells. miR-200c directly regulates the expression of IL-6, IL-8 and CCL-5 transcripts by binding to their 3'UTRs. A plasmid-based miR-200c inhibitor effectively reduces their binding activities. Additionally, miR-200c delivered using polyethylenimine (PEI) nanoparticles effectively inhibits IL-6, IL-8 and CCL-5 in primary human periodontal ligament fibroblasts and increases the biomarkers of osteogenic differentiation in human bone marrow mesenchymal stem cells (MSCs), including calcium content, ALP, and Runx2. These data demonstrate that miR-200c represses IL-6, IL-8 and CCL-5 and improves osteogenic differentiation. miR-200c may potentially be used as an effective means to prevent periodontitis-associated bone loss by arresting inflammation and osteoclastogenesis and enhancing bone regeneration. PMID:27529418

  10. Nuclear Melt Glass Dissolution and Secondary Mineral Precipitation at 40 to 200C

    SciTech Connect

    Zavarin, M; Roberts, S; Viani, B; Pawloski, G; Rose, T

    2004-06-14

    Most long-lived radionuclides associated with an underground nuclear test are initially incorporated into melt glass and become part of the hydrologic source term (HST) only upon their release via glass dissolution (Pawloski et al., 2001). As the melt glass dissolves, secondary minerals precipitate. The types of secondary minerals that precipitate influence the water chemistry in and around the melt glass. The secondary minerals also provide a sorption sink to the released radionuclides. The changing water chemistry affects the rate of glass dissolution; it also affects the sorption behavior of the released radionuclides. This complex nature of glass dissolution and its important role in defining the HST requires a thorough understanding of glass dissolution and secondary mineral precipitation. The identity of secondary minerals formed at temperatures from 40 to 200 C are evaluated in this report to assist in that understanding.

  11. miR-200c Inhibits Melanoma Progression and Drug Resistance through Down-Regulation of Bmi-1

    PubMed Central

    Liu, Shujing; Tetzlaff, Michael T.; Cui, Rutao; Xu, Xiaowei

    2013-01-01

    MicroRNAs (miRNAs) are short noncoding RNAs that play crucial roles in tumorigenesis and tumor progression. Melanoma is the most aggressive skin cancer that is resistant or rapidly develops resistance to a variety of chemotherapeutic agents. The role of miRNAs in melanoma progression and drug resistance has not been well studied. Herein, we demonstrate that miR-200c is down-regulated in melanomas (primary and metastatic) compared with melanocytic nevi. Overexpression of miR-200c in melanoma cells resulted in significantly decreased cell proliferation and migratory capacity as well as drug resistance. miR-200c overexpression resulted in significant down-regulation of BMI-1, ABCG2, ABCG5, and MDR1 expression and in a concomitant increase in E-cadherin levels. Knockdown of BMI-1 showed similar effects as miR-200c overexpression in melanoma cells. In addition, miR-200c overexpression significantly inhibited melanoma xenograft growth and metastasis in vivo, and this correlated with diminished expression of BMI-1 and reduced levels of E-cadherin in these tumors. The effects of miR-200c on melanoma cell proliferation and migratory capacity and on self-renewal were rescued by overexpression of Bmi-1, and the reversal of these phenotypes correlated with a reduction in E-cadherin expression and increased levels of ABCG2, ABCG5, and MDR1. Taken together, these findings demonstrate a key role for miR-200c in melanoma progression and drug resistance. These results suggest that miR-200c may represent a critical target for increasing melanoma sensitivity to clinical therapies. PMID:22982443

  12. MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis

    PubMed Central

    Hur, Keun; Toiyama, Yuji; Takahashi, Masanobu; Balaguer, Francesc; Nagasaka, Takeshi; Koike, Junichi; Hemmi, Hiromichi; Koi, Minoru; Boland, C Richard; Goel, Ajay

    2013-01-01

    Objective Distant metastasis is the major cause of cancer-related death in patients with colorectal cancer (CRC). Although the microRNA-200 (miR-200) family is a crucial inhibitor of epithelial-to-mesenchymal transition (EMT) in human cancer, the role of miR-200 members in the pathogenesis of metastatic CRC has not been investigated. Design Fifty-four pairs of primary CRC and corresponding matched liver metastasis tissue specimens were analysed for expression and methylation status of the miR-200 family members. Functional analysis of miR-200c overexpression was investigated in CRC cell lines, and cells were analysed for proliferation, invasion and migration. Expression of several miR-200c target genes (ZEB1, ETS1 and FLT1) and EMT markers (E-cadherin and vimentin) in CRC cell lines and tissue specimens was validated. Results Liver metastasis tissues showed higher expression of miR-200c (primary CRC=1.31 vs. liver metastasis=1.59; p=0.0014) and miR-141 (primary CRC=0.14 vs. liver metastasis=0.17; p=0.0234) than did primary CRCs, which was significantly associated with hypomethylation of the promoter region of these miRNAs (primary CRC=61.2% vs. liver metastasis=46.7%; p<0.0001). The invasive front in primary CRC tissues revealed low miR-200c expression by in situ hybridization analysis. Transfection of miR-200c precursors resulted in enhanced cell proliferation but reduced invasion and migration behaviours in CRC cell lines. Overexpression of miR-200c in CRC cell lines caused reduced expression of putative gene targets, and resulted in increased E-cadherin and reduced vimentin expression. The associations between miR-200c, target genes and EMT markers were validated in primary CRCs and matching liver metastasis tissues. Conclusions miR-200c plays an important role in mediating EMT and metastatic behaviour in the colon. Its expression is epigenetically regulated, and miR-200c may serve as a potential diagnostic marker and therapeutic target for patients with CRC

  13. Regulation of miR-200c/141 expression by intergenic DNA-looping and transcriptional read-through.

    PubMed

    Batista, Luciana; Bourachot, Brigitte; Mateescu, Bogdan; Reyal, Fabien; Mechta-Grigoriou, Fatima

    2016-01-01

    The miR-200 family members have been implicated in stress responses and ovarian tumorigenesis. Here, we find that miR-200c/141 transcription is intimately linked to the transcription of the proximal upstream gene PTPN6 (SHP1) in all physiological conditions tested. PTPN6 and miR-200c/141 are transcriptionally co-regulated by two complementary mechanisms. First, a bypass of the regular PTPN6 polyadenylation signal allows the transcription of the downstream miR-200c/141. Second, the promoters of the PTPN6 and miR-200c/141 transcription units physically interact through a 3-dimensional DNA loop and exhibit similar epigenetic regulation. Our findings highlight that transcription of intergenic miRNAs is a novel outcome of transcriptional read-through and reveal a yet unexplored type of DNA loop associating two closely located promoters. These mechanisms have significant relevance in ovarian cancers and stress response, pathophysiological conditions in which miR-200c/141 exert key functions. PMID:26725650

  14. miR-200c and phospho-AKT as prognostic factors and mediators of osteosarcoma progression and lung metastasis.

    PubMed

    Berlanga, Pablo; Muñoz, Lisandra; Piqueras, Marta; Sirerol, J Antoni; Sánchez-Izquierdo, María Dolors; Hervás, David; Hernández, Miguel; Llavador, Margarita; Machado, Isidro; Llombart-Bosch, Antonio; Cañete, Adela; Castel, Victoria; Font de Mora, Jaime

    2016-08-01

    Lung metastasis is the major cause of death in osteosarcoma patients. However, molecular mechanisms underlying this metastasis remain poorly understood. To identify key molecules related with pulmonary metastasis of pediatric osteosarcomas, we analyzed high-throughput miRNA expression in a cohort of 11 primary tumors and 15 lung metastases. Results were further validated with an independent cohort of 10 primary tumors and 6 metastases. In parallel, we performed immunohistochemical analysis of activated signaling pathways in 36 primary osteosarcomas. Only phospho-AKT associated with lower overall survival in primary tumors, supporting its role in osteosarcoma progression. CTNNB1 expression also associated with lower overall survival but was not strong enough to be considered an independent variable. Interestingly, miR-200c was overexpressed in lung metastases, implicating an inhibitory feed-back loop to PI3K-AKT. Moreover, transfection of miR200c-mimic in U2-OS cells reduced phospho-AKT levels but increased cellular migration and proliferation. Notably, miR-200c expression strongly correlated with miR-141 and with the osteogenic inhibitor miR-375, all implicated in epithelial to mesenchymal transition. These findings contrast epithelial tumors where reduced miR-200c expression promotes metastasis. Indeed, we noted that osteosarcoma cells in the lung also expressed the epithelial marker CDH1, revealing a change in their mesenchymal phenotype. We propose that miR-200c upregulation occurs late in osteosarcoma progression to provide cells with an epithelial phenotype that facilitates their integration in the metastatic lung niche. Thus, our findings identify phospho-AKT in the primary tumor and miR-200c later during tumor progression as prognostic molecules and potential therapeutic targets to prevent progression and metastasis of pediatric osteosarcomas. PMID:27155790

  15. Combustion Power Unit--400: CPU-400.

    ERIC Educational Resources Information Center

    Combustion Power Co., Palo Alto, CA.

    Aerospace technology may have led to a unique basic unit for processing solid wastes and controlling pollution. The Combustion Power Unit--400 (CPU-400) is designed as a turboelectric generator plant that will use municipal solid wastes as fuel. The baseline configuration is a modular unit that is designed to utilize 400 tons of refuse per day…

  16. MicroRNA-200c-141 and ∆Np63 are required for breast epithelial differentiation and branching morphogenesis.

    PubMed

    Hilmarsdóttir, Bylgja; Briem, Eirikur; Sigurdsson, Valgardur; Franzdóttir, Sigrídur Rut; Ringnér, Markus; Arason, Ari Jon; Bergthorsson, Jon Thor; Magnusson, Magnus Karl; Gudjonsson, Thorarinn

    2015-07-15

    The epithelial compartment of the breast contains two lineages, the luminal- and the myoepithelial cells. D492 is a breast epithelial cell line with stem cell properties that forms branching epithelial structures in 3D culture with both luminal- and myoepithelial differentiation. We have recently shown that D492 undergo epithelial to mesenchymal transition (EMT) when co-cultured with endothelial cells. This 3D co-culture model allows critical analysis of breast epithelial lineage development and EMT. In this study, we compared the microRNA (miR) expression profiles for D492 and its mesenchymal-derivative D492M. Suppression of the miR-200 family in D492M was among the most profound changes observed. Exogenous expression of miR-200c-141 in D492M reversed the EMT phenotype resulting in gain of luminal but not myoepithelial differentiation. In contrast, forced expression of ∆Np63 in D492M restored the myoepithelial phenotype only. Co-expression of miR-200c-141 and ∆Np63 in D492M restored the branching morphogenesis in 3D culture underlining the requirement for both luminal and myoepithelial elements for obtaining full branching morphogenesis in breast epithelium. Introduction of a miR-200c-141 construct in both D492 and D492M resulted in resistance to endothelial induced EMT. In conclusion, our data suggests that expression of miR-200c-141 and ∆Np63 in D492M can reverse EMT resulting in luminal- and myoepithelial differentiation, respectively, demonstrating the importance of these molecules in epithelial integrity in the human breast. PMID:25967125

  17. Correlation between EGFR Amplification and the Expression of MicroRNA-200c in Primary Glioblastoma Multiforme

    PubMed Central

    Serna, Eva; Lopez-Gines, Concha; Monleon, Daniel; Muñoz-Hidalgo, Lisandra; Callaghan, Robert C.; Gil-Benso, Rosario; Martinetto, Horacio; Gregori-Romero, Aurelia; Gonzalez-Darder, Jose; Cerda-Nicolas, Miguel

    2014-01-01

    Extensive infiltration of the surrounding healthy brain tissue is a critical feature in glioblastoma. Several miRNAs have been related to gliomagenesis, some of them related with the EGFR pathway. We have evaluated whole-genome miRNA expression profiling associated with different EGFR amplification patterns, studied by fluorescence in situ hybridization in tissue microarrays, of 30 cases of primary glioblastoma multiforme, whose clinicopathological and immunohistochemical features have also been analyzed. MicroRNA-200c showed a very significant difference between tumors having or not EGFR amplification. This microRNA plays an important role in epithelial-mesenchymal transition, but its implication in the behavior of glioblastoma is largely unknown. With respect to EGFR status our cases were categorized into three groups: high level EGFR amplification, low level EGFR amplification, and no EGFR amplification. Our results showed that microRNA-200c and E-cadherin expression are down-regulated, while ZEB1 is up-regulated, when tumors showed a high level of EGFR amplification. Conversely, ZEB1 mRNA expression levels were significantly lower in the group of tumors without EGFR amplification. Tumors with a low level of EGFR amplification showed ZEB1 expression levels comparable to those detected in the group with a high level of amplification. In this study we provide what is to our knowledge the first report of association between microRNA-200c and EGFR amplification in glioblastomas. PMID:25058589

  18. Role for DNA methylation in the regulation of miR-200c and miR-141 expression in normal and cancer cells

    SciTech Connect

    Vrba, Lukas; Jensen, Taylor J.; Garbe, James C.; Heimark, Ronald L.; Cress, Anne E.; Dickinson, Sally; Stampfer, Martha R.; Futscher, Bernard W.

    2009-12-23

    BACKGROUND: The microRNA-200 family participates in the maintenance of an epithelial phenotype and loss of its expression can result in epithelial to mesenchymal transition (EMT). Furthermore, the loss of expression of miR-200 family members is linked to an aggressive cancer phenotype. Regulation of the miR-200 family expression in normal and cancer cells is not fully understood. METHODOLOGY/ PRINCIPAL FINDINGS: Epigenetic mechanisms participate in the control of miR-200c and miR-141 expression in both normal and cancer cells. A CpG island near the predicted mir-200c/mir-141 transcription start site shows a striking correlation between miR-200c and miR-141 expression and DNA methylation in both normal and cancer cells, as determined by MassARRAY technology. The CpG island is unmethylated in human miR-200/miR-141 expressing epithelial cells and in miR-200c/miR-141 positive tumor cells. The CpG island is heavily methylated in human miR-200c/miR-141 negative fibroblasts and miR-200c/miR-141 negative tumor cells. Mouse cells show a similar inverse correlation between DNA methylation and miR-200c expression. Enrichment of permissive histone modifications, H3 acetylation and H3K4 trimethylation, is seen in normal miR-200c/miR-141-positive epithelial cells, as determined by chromatin immunoprecipitation coupled to real-time PCR. In contrast, repressive H3K9 dimethylation marks are present in normal miR-200c/miR-141-negative fibroblasts and miR-200c/miR-141 negative cancer cells and the permissive histone modifications are absent. The epigenetic modifier drug, 5-aza-2'-deoxycytidine, reactivates miR-200c/miR-141 expression showing that epigenetic mechanisms play a functional role in their transcriptional control. CONCLUSIONS/ SIGNIFICANCE: We report that DNA methylation plays a role in the normal cell type-specific expression of miR-200c and miR-141 and this role appears evolutionarily conserved, since similar results were obtained in mouse. Aberrant DNA methylation of the

  19. The ZEB1/miR-200c feedback loop regulates invasion via actin interacting proteins MYLK and TKS5

    PubMed Central

    Stemmler, Marc P.; Kleemann, Julia A.; Brabletz, Thomas; Brabletz, Simone

    2015-01-01

    Epithelial to mesenchymal transition (EMT) is a developmental process which is aberrantly activated during cancer invasion and metastasis. Elevated expression of EMT-inducers like ZEB1 enables tumor cells to detach from the primary tumor and invade into the surrounding tissue. The main antagonist of ZEB1 in controlling EMT is the microRNA-200 family that is reciprocally linked to ZEB1 in a double negative feedback loop. Here, we further elucidate how the ZEB1/miR-200 feedback loop controls invasion of tumor cells. The process of EMT is attended by major changes in the actin cytoskeleton. Via in silico screening of genes encoding for actin interacting proteins, we identified two novel targets of miR-200c - TKS5 and MYLK (MLCK). Co-expression of both genes with ZEB1 was observed in several cancer cell lines as well as in breast cancer patients and correlated with low miR-200c levels. Depletion of TKS5 or MYLK in breast cancer cells reduced their invasive potential and their ability to form invadopodia. Whereas TKS5 is known to be a major component, we could identify MYLK as a novel player in invadopodia formation. In summary, TKS5 and MYLK represent two mediators of invasive behavior of cancer cells that are regulated by the ZEB1/miR-200 feedback loop. PMID:26334100

  20. miR-200c Regulation of Metastases in Ovarian Cancer: Potential Role in Epithelial and Mesenchymal Transition

    PubMed Central

    Sulaiman, Siti A.; Ab Mutalib, Nurul-Syakima; Jamal, Rahman

    2016-01-01

    Among the gynecological malignancies, ovarian cancer is the most fatal due to its high mortality rate. Most of the identified cases are epithelial ovarian cancer (EOC) with five distinct subtypes: high-grade serous carcinoma, low-grade serous carcinoma, mucinous carcinoma, endometrioid carcinoma, and clear-cell carcinoma. Lack of an early diagnostic approach, high incidence of tumor relapse and the heterogenous characteristics between each EOC subtypes contribute to the difficulties in developing precise intervention and therapy for the patients. MicroRNAs (miRNAs) are single-stranded RNAs that have been shown to function as tumor suppressors or oncomiRs. The miR-200 family, especially miR-200c, has been shown to be implicated in the metastasis and invasion of ovarian carcinoma due to its functional regulation of epithelial-to-mesenchymal transition (EMT). This mini review is aimed to summarize the recent findings of the miR-200c functional role as well as its validated targets in the metastasis cascade of ovarian cancer, with a focus on EMT regulation. The potential of this miRNA in early diagnosis and its dual expression status are also discussed. PMID:27601996

  1. miR-200c-SUMOylated KLF4 feedback loop acts as a switch in transcriptional programs that control VSMC proliferation.

    PubMed

    Zheng, Bin; Bernier, Michel; Zhang, Xin-hua; Suzuki, Toru; Nie, Chan-quan; Li, Yong Hui; Zhang, Yong; Song, Li-Li; Shi, Hui-jing; Liu, Yan; Zheng, Cui-ying; Wen, Jin-kun

    2015-05-01

    The regulation of vascular smooth muscle cell (VSMC) proliferation is an important issue because it has major implications for the prevention of pathological vascular conditions. Using microRNA array screen, we found the expression levels of 200 unique miRNAs in hyperplasic tissues. Among them, miR-200c expression substantially was down-regulated. The objective of this work was to assess the function of miR-200c and SUMOylated Krϋppel-like transcription factor 4 (KLF4) in the regulation of VSMC proliferation in both cultured cells and animal models of balloon injury. Under basal conditions, we found that miR-200c inhibited the expression of KLF4 and the SUMO-conjugating enzyme Ubc9. Upon PDGF-BB treatment, Ubc9 interacted with and promoted the SUMOylation of KLF4, which allowed the recruitment of transcriptional corepressors (e.g., nuclear receptor corepressor (NCoR) and HDAC2) to the miR-200c promoter. The reduction in miR-200c levels led to increased target gene expression (e.g., Ubc9 and KLF4), which further repressed miR-200c levels and accelerated VSMC proliferation. These results demonstrate that induction of a miR-200c-SUMOylated KLF4 feedback loop is a significant aspect of the PDGF-BB proliferative response in VSMCs and that targeting Ubc9 represents a novel approach for the prevention of restenosis. PMID:25791170

  2. miR200c attenuates P-gp-mediated MDR and metastasis by targeting JNK2/c-Jun signaling pathway in colorectal cancer.

    PubMed

    Sui, Hua; Cai, Guo-Xiang; Pan, Shu-Fang; Deng, Wan-Li; Wang, Yu-Wei; Chen, Zhe-Sheng; Cai, San-Jun; Zhu, Hui-Rong; Li, Qi

    2014-12-01

    MicroRNA-200c (miR200c) recently emerged as an important regulator of tumorigenicity and cancer metastasis; however, its role in regulating multidrug resistance (MDR) remains unknown. In the current study, we found that the expression levels of miR200c in recurrent and metastatic colorectal cancers were significantly lower, whereas the JNK2 expression was higher compared with primary tumors. We showed that in MDR colorectal cancer cells, miR200c targeted the 3' untranslated region of the JNK2 gene. Overexpression of miR200c attenuated the levels of p-JNK, p-c-Jun, P-gp, and MMP-2/-9, the downstream factors of the JNK signaling pathway, resulting in increased sensitivity to chemotherapeutic drugs, which was accompanied by heightened apoptosis and decreased cell invasion and migration. Moreover, in an orthotopic MDR colorectal cancer mouse model, we demonstrated that overexpression of miR200c effectively inhibited the tumor growth and metastasis. At last, in the tumor samples from patients with locally advanced colorectal cancer with routine postsurgical chemotherapy, we observed an inverse correlation between the levels of mRNA expression of miR200c and JNK2, ABCB1, and MMP-9, thus predicting patient therapeutic outcomes. In summary, we found that miR200c negatively regulated the expression of JNK2 gene and increased the sensitivity of MDR colorectal cancer cells to chemotherapeutic drugs, via inhibiting the JNK2/p-JNK/p-c-Jun/ABCB1 signaling. Restoration of miR200c expression in MDR colorectal cancer may serve as a promising therapeutic approach in MDR-induced metastasis. PMID:25205654

  3. Electroneutral composite polymersomes self-assembled by amphiphilic polyphosphazenes for effective miR-200c in vivo delivery to inhibit drug resistant lung cancer.

    PubMed

    Peng, Yan; Zhu, Xiumei; Qiu, Liyan

    2016-11-01

    MiR-200c has been confirmed to display remarkable effects on proliferation inhibition and apoptosis induction of certain cancer cells, but the main challenge for its successful translation into the clinic remains its effective delivery to the action site in vivo. In this study, a novel composite polyphosphazene vesicle system composed of amphiphilic [NP(PEG)0.3(EAB)1.7]n (PEEP) and weakly cationic [NP(PEG)0.5(DPA)1.5]n (PEDP) was prepared via a very simple dialysis method. The loading of miR-200c was accomplished with high efficiency by taking advantage of the combination effect of physical encapsulation and electrostatic interaction between vectors and miR-200c. The resultant miR-200c-loaded PEEP-PEDP polymersome (Nano-ED-200c) displayed suitable particle size, electric neutrality, excellent Ribonuclease stability and hemocompatibility. We also evaluated its subsequent miR-200c function in paclitaxel resistance human lung cancer (A549/T) cells in culture and tumor xenografts in nude mice. The results showed that Nano-ED-200c could achieve a higher miR-200c level and the enhanced antitumor efficacy with 68% tumor inhibition ratio at a very low dose of 1.0 mg/kg than PEEP nanoparticle, PEDP nanoparticle, even than Lipo2000. All these evidences indicated that this miR-200c delivery via polyphosphazene vesicles could act as a potential new therapeutic option for paclitaxel resistant human lung cancer. PMID:27541441

  4. miR-200c/Bmi1 axis and epithelial–mesenchymal transition contribute to acquired resistance to BRAF inhibitor treatment

    PubMed Central

    Liu, Shujing; Tetzlaff, Michael T.; Wang, Tao; Yang, Ruifeng; Xie, Lin; Zhang, Gao; Krepler, Clemens; Xiao, Min; Beqiri, Marilda; Xu, Wei; Karakousis, Giorgos; Schuchter, Lynn; Amaravadi, Ravi K.; Xu, Weiting; Wei, Zhi; Herlyn, Meenhard; Yao, Yuan; Zhang, Litao; Wang, Yingjie; Zhang, Lin; Xu, Xiaowei

    2015-01-01

    Summary Resistance to BRAF inhibitors (BRAFi) is one of the major challenges for targeted therapies for BRAF-mutant melanomas. However, little is known about the role of microRNAs in conferring BRAFi resistance. Herein, we demonstrate that miR-200c expression is significantly reduced whereas miR-200c target genes including Bmi1, Zeb2, Tubb3, ABCG5, and MDR1 are significantly increased in melanomas that acquired BRAFi resistance compared to pretreatment tumor biopsies. Similar changes were observed in BRAFi-resistant melanoma cell lines. Overexpression of miR-200c or knock-down of Bmi1 in resistant melanoma cells restores their sensitivities to BRAFi, leading to deactivation of the PI3K/AKT and MAPK signaling cascades, and acquisition of epithelial– mesenchymal transition-like phenotypes, including upregulation of E-cadherin, downregulation of N-cadherin, and ABCG5 and MDR1 expression. Conversely, knock-down of miR-200c or overexpression of Bmi1 in BRAFi-sensitive melanoma cells activates the PI3K/AKT and MAPK pathways, upregulates N-cadherin, ABCG5, and MDR1 expression, and downregulates E-cadherin expression, leading to BRAFi resistance. Together, our data identify miR-200c as a critical signaling node in BRAFi-resistant melanomas impacting the MAPK and PI3K/AKT pathways, suggesting miR-200c as a potential therapeutic target for overcoming acquired BRAFi resistance. PMID:25903073

  5. MiR-200c overexpression is associated with better efficacy of EGFR-TKIs in non-small cell lung cancer patients with EGFR wild-type

    PubMed Central

    Li, Jiayu; Li, Xuefei; Ren, Shengxiang; Chen, Xiaoxia; Zhang, Yishi; Zhou, Fei; Zhao, Mingchuan; Zhao, Chao; Chen, Xiu; Cheng, Ningning; Zhao, Yinmin; Zhou, Caicun; Hirsch, Fred R.

    2014-01-01

    Several randomized trials have demonstrated non-small cell lung cancer (NSCLC) patients with activating epidermal growth factor receptor (EGFR) mutations can achieve favorable clinical outcomes on treatment with EGFR tyrosine kinase inhibitors (TKIs). EGFR mutation is considered as a predictive marker for efficacy of EGFR-TKIs in NSCLC. Here we show miR-200c overexpression was correlated with the epithelial phenotype and sensitivity to gefitinib in EGFR wild-type NSCLC cell lines. Up-regulated miR-200c could regain the sensitivity to gefitinib in the EGFR wild-type cell lines and miR-200c could regulate epithelial to mesenchymal transition through PI3K/AKT and MEK/ERK pathways. NSCLC patients at advanced stage (N=150) who received EGFR-TKIs (gefitinib or erlotinib) as second- or third-line therapy from September 2008 to December 2012 were included in the study. In 66 NSCLC patients with wild-type EGFR, high levels of miR-200c expression was associated with higher disease control rate (DCR), longer progression-free survival (PFS) and longer overall survival (OS) compared with low miR-200c expression subgroup. In the subgroup with EGFR mutation, the trend remained the same but not statistically significant. Overall, these findings indicated that miR-200c might be a predictive biomarker for sensitivity to EGFR-TKIs in advanced NSCLC patients with wild-type EGFR. PMID:25277203

  6. 300 Area Disturbance Report

    SciTech Connect

    LL Hale; MK Wright; NA Cadoret

    1999-01-07

    and white photographs provide a partial record of some excavations, including trenches, building basements, and material lay-down yards. Estimates of excavation depth and width can be made, but these estimates are not accurate enough to pinpoint the exact location where the disturbedhmdisturbed interface is located (e.g., camera angles were such that depths and/or widths of excavations could not be accurately determined or estimated). In spite of these limitations, these photographs provide essential information. Aerial and historic low-level photographs have captured what appears to be backfill throughout much of the eastern portion of the 300 Area-near the Columbia River shoreline. This layer of fill has likely afforded some protection for the natural landscape buried beneath the fill. This assumption fits nicely with the intermittent and inadvertent discoveries of hearths and stone tools documented through the years in this part of the 300 Area. Conversely, leveling of sand dunes appears to be substantial in the northwestern portion of the 300 Area during the early stages of development. o Project files and engineer drawings do not contain information on any impromptu but necessary adjustments made on the ground during project implementation-after the design phase. Further, many projects are planned and mapped but never implemented-this information is also not often placed in project files. Specific recommendations for a 300 Area cultural resource monitoring strategy are contained in the final section of this document. In general, it is recommended that monitoring continue for all projects located within 400 m of the Columbia River. The 400-m zone is culturally sensitive and likely retains some of the most intact buried substrates in the 300 Area.

  7. Enhancement of radiotherapy efficacy by miR-200c-loaded gelatinase-stimuli PEG-Pep-PCL nanoparticles in gastric cancer cells.

    PubMed

    Cui, Fang-bo; Liu, Qin; Li, Ru-Tian; Shen, Jie; Wu, Pu-yuan; Yu, Li-Xia; Hu, Wen-jing; Wu, Feng-lei; Jiang, Chun-Ping; Yue, Guo-feng; Qian, Xiao-Ping; Jiang, Xi-Qun; Liu, Bao-Rui

    2014-01-01

    Radiotherapy is the main locoregional control modality for many types of unresectable tumors, including gastric cancer. However, many patients fail radiotherapy due to intrinsic radioresistance of cancer cells, which has been found to be strongly associated with cancer stem cell (CSC)-like properties. In this study, we developed a nanoparticle formulation to deliver miR-200c, which is reported to inhibit CSC-like properties, and then evaluated its potential activity as a radiosensitizer. miR-200c nanoparticles significantly augmented radiosensitivity in three gastric cancer cell lines (sensitization enhancement ratio 1.13-1.25), but only slightly in GES-1 cells (1.06). In addition to radioenhancement, miR-200c nanoparticles reduced the expression of CD44, a putative CSC marker, and the percentage of CD44(+) BGC823 cells. Meanwhile, other CSC-like properties, including invasiveness and resistance to apoptosis, could be suppressed by miR-200c nanoparticles. CSC-associated radioresistance mechanisms, involving reactive oxygen species levels and DNA repair capacity, were also attenuated. We have demonstrated that miR-200c nanoparticles are an effective radiosensitizer in gastric cancer cells and induce little radiosensitization in normal cells, which suggests that they are as a promising candidate for further preclinical and clinical evaluation. PMID:24872697

  8. 34 CFR 300.300 - Parental consent.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Parental consent. 300.300 Section 300.300 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH DISABILITIES Evaluations,...

  9. Knockdown of lncRNA-ATB suppresses autocrine secretion of TGF-β2 by targeting ZNF217 via miR-200c in keloid fibroblasts

    PubMed Central

    Zhu, Hua-Yu; Bai, Wen-Dong; Li, Chao; Zheng, Zhao; Guan, Hao; Liu, Jia-Qi; Yang, Xue-Kang; Han, Shi-Chao; Gao, Jian-Xin; Wang, Hong-Tao; Hu, Da-Hai

    2016-01-01

    Abnormally high activation of transforming growth factor-β (TGF-β) signaling has been demonstrated to be involved in the initiation and progression of keloids. However, the functional role of long non-coding RNA (lncRNA)-activated by TGF-β (lncRNA-ATB) in keloids has not been documented. Here we investigated the role of lncRNA-ATB in the autocrine secretion of TGF-β in keloid fibroblasts (KFs) and explored the underlying molecular mechanism. Using immunohistochemistry and quantitative RT-PCR analysis, we showed that lncRNA-ATB and ZNF217, a transcriptional activator of TGF-β, were overexpressed and miR-200c, which targets ZNF217, was under-expressed in keloid tissue and keloid fibroblasts. Through gain- and loss-of-function studies, we demonstrated that knockdown of lncRNA-ATB decreased autocrine secretion of TGF-β2 and ZNF217 expression but upregulated expression of miR-200c in KFs. Stable downregulation of ZNF217 expression decreased the autocrine secretion of TGF-β2. miR-200c was endogenously associated with lncRNA-ATB, and inhibition of miR-200c overcame the decrease in ZNF217 expression in KFs. Taken together, these findings indicate that lncRNA-ATB governs the autocrine secretion of TGF-β2 in KFs, at least in part, by downregulating the expression level of ZNF217 via miR-200c, suggesting a signaling axis consisting of lncRNA-ATB/miR-200c/ZNF217/TGF-β2. These findings may provide potential biomarkers and targets for novel diagnostic and therapeutic approaches for keloids. PMID:27090737

  10. Knockdown of lncRNA-ATB suppresses autocrine secretion of TGF-β2 by targeting ZNF217 via miR-200c in keloid fibroblasts.

    PubMed

    Zhu, Hua-Yu; Bai, Wen-Dong; Li, Chao; Zheng, Zhao; Guan, Hao; Liu, Jia-Qi; Yang, Xue-Kang; Han, Shi-Chao; Gao, Jian-Xin; Wang, Hong-Tao; Hu, Da-Hai

    2016-01-01

    Abnormally high activation of transforming growth factor-β (TGF-β) signaling has been demonstrated to be involved in the initiation and progression of keloids. However, the functional role of long non-coding RNA (lncRNA)-activated by TGF-β (lncRNA-ATB) in keloids has not been documented. Here we investigated the role of lncRNA-ATB in the autocrine secretion of TGF-β in keloid fibroblasts (KFs) and explored the underlying molecular mechanism. Using immunohistochemistry and quantitative RT-PCR analysis, we showed that lncRNA-ATB and ZNF217, a transcriptional activator of TGF-β, were overexpressed and miR-200c, which targets ZNF217, was under-expressed in keloid tissue and keloid fibroblasts. Through gain- and loss-of-function studies, we demonstrated that knockdown of lncRNA-ATB decreased autocrine secretion of TGF-β2 and ZNF217 expression but upregulated expression of miR-200c in KFs. Stable downregulation of ZNF217 expression decreased the autocrine secretion of TGF-β2. miR-200c was endogenously associated with lncRNA-ATB, and inhibition of miR-200c overcame the decrease in ZNF217 expression in KFs. Taken together, these findings indicate that lncRNA-ATB governs the autocrine secretion of TGF-β2 in KFs, at least in part, by downregulating the expression level of ZNF217 via miR-200c, suggesting a signaling axis consisting of lncRNA-ATB/miR-200c/ZNF217/TGF-β2. These findings may provide potential biomarkers and targets for novel diagnostic and therapeutic approaches for keloids. PMID:27090737

  11. MiR-200b/200c/429 subfamily negatively regulates Rho/ROCK signaling pathway to suppress hepatocellular carcinoma metastasis.

    PubMed

    Wong, Chun-Ming; Wei, Lai; Au, Sandy Leung-Kuen; Fan, Dorothy Ngo-Yin; Zhou, Yuan; Tsang, Felice Ho-Ching; Law, Cheuk-Ting; Lee, Joyce Man-Fong; He, Xianghuo; Shi, Jue; Wong, Carmen Chak-Lui; Ng, Irene Oi-Lin

    2015-05-30

    MiR-200 family is an important regulator of epithelial-mesenchymal transition and has been implicated in human carcinogenesis. However, their expression and functions in human cancers remain controversial. In the work presented here, we showed that miR-200 family members were frequently down-regulated in hepatocellular carcinoma (HCC). Although all five members of miR-200 family inhibited ZEB1/2 expression in HCC cell lines, we showed that overexpression only of the miR-200b/200c/429 subfamily, but not the miR-200a/141 subfamily, resulted in impeded HCC cell migration. Further investigations led to the identification of RhoA and ROCK2 as specific down-stream targets of the miR-200b/200c/429 subfamily. We demonstrated that the miR-200b/200c/429 subfamily inhibited HCC cell migration through modulating Rho/ROCK mediated cell cytoskeletal reorganization and cell-substratum adhesion. Re-expression of miR-200b significantly suppressed lung metastasis of HCC cells in an orthotopic liver implantation model in vivo. In conclusion, our findings identified the miR-200b/200c/429 subfamily as metastasis suppressor microRNAs in human HCC and highlighted the functional discrepancy among miR-200 family members. PMID:25909223

  12. 20 CFR 664.205 - How is the “deficient in basic literacy skills” criterion in § 664.200(c)(1) defined and documented?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 4 2013-04-01 2013-04-01 false How is the âdeficient in basic literacy... WORKFORCE INVESTMENT ACT Eligibility for Youth Services § 664.205 How is the “deficient in basic literacy... documentation requirements regarding the “deficient in basic literacy skills” criterion in § 664.200(c)(1)...

  13. 20 CFR 664.205 - How is the “deficient in basic literacy skills” criterion in § 664.200(c)(1) defined and documented?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 4 2012-04-01 2012-04-01 false How is the âdeficient in basic literacy... WORKFORCE INVESTMENT ACT Eligibility for Youth Services § 664.205 How is the “deficient in basic literacy... documentation requirements regarding the “deficient in basic literacy skills” criterion in § 664.200(c)(1)...

  14. 20 CFR 664.205 - How is the “deficient in basic literacy skills” criterion in § 664.200(c)(1) defined and documented?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false How is the âdeficient in basic literacy... INVESTMENT ACT Eligibility for Youth Services § 664.205 How is the “deficient in basic literacy skills... requirements regarding the “deficient in basic literacy skills” criterion in § 664.200(c)(1) may be...

  15. Transcriptomic Profiling Reveals Hepatic Stem-like Gene Signatures and Interplay of miR-200c and EMT in Intrahepatic Cholangiocarcinoma

    PubMed Central

    Oishi, Naoki; Kumar, Mia R.; Roessler, Stephanie; Ji, Junfang; Forgues, Marshonna; Budhu, Anuradha; Zhao, Xuelian; Andersen, Jesper B.; Ye, Qing-Hai; Jia, Hu-Liang; Qin, Lun-Xiu; Yamashita, Taro; Woo, Hyun Goo; Kim, Yoon Jun; Kaneko, Shuichi; Tang, Zhao-You; Thorgeirsson, Snorri S.; Wang, Xin Wei

    2012-01-01

    Intrahepatic cholangiocellular carcinoma (ICC) is the second most common type of primary liver cancer. However, its tumor heterogeneity and molecular characteristics are largely unknown. In this study, we conducted transcriptomic profiling of 23 ICC and combined hepatocellular cholangiocarcinoma tumor specimens from Asian patients using Affymetrix mRNA and Nanostring microRNA microarrays to search for unique gene signatures linked to tumor subtypes and patient prognosis. We validated the signatures in additional 68 ICC cases derived from Caucasian patients. We found that both mRNA and microRNA expression profiles could independently classify Asian ICC cases into two main subgroups, one of which shared gene expression signatures with previously identified hepatocellular carcinoma (HCC) with stem cell gene expression traits. ICC-specific gene signatures could predict survival in Asian HCC cases and independently in Caucasian ICC cases. Integrative analyses of the ICC-specific mRNA and microRNA expression profiles revealed that a common signaling pathway linking miR-200c signaling to epithelial-mesenchymal transition (EMT) was preferentially activated in ICC with stem cell gene expression traits. Inactivation of miR-200c resulted in an induction of EMT while activation of miR-200c led to a reduction of EMT including a reduced cell migration and invasion in ICC cells. We also found that miR-200c and NCAM1 expression were negatively correlated and their expression levels were predictive of survival in ICC samples. NCAM1, a known hepatic stem/progenitor cell marker, was experimentally demonstrated to be a direct target of miR-200c. Conclusion: Our results indicate that ICC and HCC share common stem-like molecular characteristics and poor prognosis. We suggest that the specific components of EMT may be exploited as critical biomarkers and clinically relevant therapeutic targets for an aggressive form of stem cell-like ICC. PMID:22707408

  16. "The 400 Blows."

    ERIC Educational Resources Information Center

    McLaughlin, Frank

    1967-01-01

    Francois Truffaut's film classic, "The 400 Blows," can provide artistic material for English teachers who wish to design a unit on the individual and society, on alienation, or on growing up. In the film, a powerful image of the terrifying isolation that the adolescent, Doinel, experiences is presented through repeated camera shots of symbolic…

  17. 7 CFR 400.49-400.50 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 6 2012-01-01 2012-01-01 false 400.49-400.50 Section 400.49-400.50 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Food Security Act of 1985, Implementation; Denial of...

  18. 7 CFR 400.49-400.50 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 6 2013-01-01 2013-01-01 false 400.49-400.50 Section 400.49-400.50 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Food Security Act of 1985, Implementation; Denial of...

  19. 7 CFR 400.49-400.50 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false 400.49-400.50 Section 400.49-400.50 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Food Security Act of 1985, Implementation; Denial of...

  20. 7 CFR 400.49-400.50 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false 400.49-400.50 Section 400.49-400.50 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Food Security Act of 1985, Implementation; Denial of...

  1. 7 CFR 400.49-400.50 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 6 2014-01-01 2014-01-01 false 400.49-400.50 Section 400.49-400.50 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Food Security Act of 1985, Implementation; Denial of...

  2. 45 CFR 400.20-400.21 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false 400.20-400.21 Section 400.20-400.21 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General...

  3. 45 CFR 400.20-400.21 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true 400.20-400.21 Section 400.20-400.21 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General...

  4. 45 CFR 400.20-400.21 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false 400.20-400.21 Section 400.20-400.21 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General...

  5. 45 CFR 400.20-400.21 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true 400.20-400.21 Section 400.20-400.21 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General...

  6. 45 CFR 400.20-400.21 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false 400.20-400.21 Section 400.20-400.21 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General...

  7. Detection of Drug-Induced Acute Kidney Injury in Humans Using Urinary KIM-1, miR-21, -200c, and -423.

    PubMed

    Pavkovic, Mira; Robinson-Cohen, Cassianne; Chua, Alicia S; Nicoara, Oana; Cárdenas-González, Mariana; Bijol, Vanesa; Ramachandran, Krithika; Hampson, Lucy; Pirmohamed, Munir; Antoine, Daniel J; Frendl, Gyorgy; Himmelfarb, Jonathan; Waikar, Sushrut S; Vaidya, Vishal S

    2016-07-01

    Drug-induced acute kidney injury (AKI) is often encountered in hospitalized patients. Although serum creatinine (SCr) is still routinely used for assessing AKI, it is known to be insensitive and nonspecific. Therefore, our objective was to evaluate kidney injury molecule 1 (KIM-1) in conjunction with microRNA (miR)-21, -200c, and -423 as urinary biomarkers for drug-induced AKI in humans. In a cross-sectional cohort of patients (n = 135) with acetaminophen (APAP) overdose, all 4 biomarkers were significantly (P < .004) higher not only in APAP-overdosed (OD) patients with AKI (based on SCr increase) but also in APAP-OD patients without clinical diagnosis of AKI compared with healthy volunteers. In a longitudinal cohort of patients with malignant mesothelioma receiving intraoperative cisplatin (Cp) therapy (n = 108) the 4 biomarkers increased significantly (P < .0014) over time after Cp administration, but could not be used to distinguish patients with or without AKI. Evidence for human proximal tubular epithelial cells (HPTECs) being the source of miRNAs in urine was obtained first, by in situ hybridization based confirmation of increase in miR-21 expression in the kidney sections of AKI patients and second, by increased levels of miR-21, -200c, and -423 in the medium of cultured HPTECs treated with Cp and 4-aminophenol (APAP degradation product). Target prediction analysis revealed 1102 mRNA targets of miR-21, -200c, and -423 that are associated with pathways perturbed in diverse pathological kidney conditions. In summary, we report noninvasive detection of AKI in humans by combining the sensitivity of KIM-1 along with mechanistic potentials of miR-21, -200c, and -423. PMID:27122240

  8. Enzyme Substrate Complex of the H200C Variant of Homoprotocatechuate 2,3-Dioxygenase: Mössbauer and Computational Studies.

    PubMed

    Meier, Katlyn K; Rogers, Melanie S; Kovaleva, Elena G; Lipscomb, John D; Bominaar, Emile L; Münck, Eckard

    2016-06-20

    The extradiol, aromatic ring-cleaving enzyme homoprotocatechuate 2,3-dioxygenase (HPCD) catalyzes a complex chain of reactions that involve second sphere residues of the active site. The importance of the second-sphere residue His200 was demonstrated in studies of HPCD variants, such as His200Cys (H200C), which revealed significant retardations of certain steps in the catalytic process as a result of the substitution, allowing novel reaction cycle intermediates to be trapped for spectroscopic characterization. As the H200C variant largely retains the wild-type active site structure and produces the correct ring-cleaved product, this variant presents a valuable target for mechanistic HPCD studies. Here, the high-spin Fe(II) states of resting H200C and the H200C-homoprotocatechuate enzyme-substrate (ES) complex have been characterized with Mössbauer spectroscopy to assess the electronic structures of the active site in these states. The analysis reveals a high-spin Fe(II) center in a low symmetry environment that is reflected in the values of the zero-field splitting (ZFS) (D ≈ - 8 cm(-1), E/D ≈ 1/3 in ES), as well as the relative orientations of the principal axes of the (57)Fe magnetic hyperfine (A) and electric field gradient (EFG) tensors relative to the ZFS tensor axes. A spin Hamiltonian analysis of the spectra for the ES complex indicates that the magnetization axis of the integer-spin S = 2 Fe(II) system is nearly parallel to the symmetry axis, z, of the doubly occupied dxy ground orbital deduced from the EFG and A-values, an observation, which cannot be rationalized by DFT assisted crystal-field theory. In contrast, ORCA/CASSCF calculations for the ZFS tensor in combination with DFT calculations for the EFG- and A-tensors describe the experimental data remarkably well. PMID:27275865

  9. Experimental turbine VT-400

    NASA Astrophysics Data System (ADS)

    Zitek, Pavel; Milčák, Petr; Noga, Tomáš

    2016-03-01

    The experimental air turbine VT400 is located in hall laboratories of the Department of Power System Engineering. It is a single-stage air turbine located in the suction of the compressor. It is able to solve various problems concerning the construction solution of turbine stages. The content of the article will deal mainly with the description of measurements on this turbine. The up-to-now research on this test rig will be briefly mentioned, too, as well as the description of the ongoing reconstruction.

  10. 34 CFR 300.300 - Parental consent.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... DISABILITIES Evaluations, Eligibility Determinations, Individualized Education Programs, and Educational Placements Parental Consent § 300.300 Parental consent. (a) Parental consent for initial evaluation. (1)(i) The public agency proposing to conduct an initial evaluation to determine if a child qualifies as...

  11. miR-141 and miR-200c as Markers of Overall Survival in Early Stage Non-Small Cell Lung Cancer Adenocarcinoma

    PubMed Central

    Campayo, Marc; Viñolas, Nuria; Marrades, Ramon M.; Cordeiro, Anna; Ruíz-Martínez, Marc; Santasusagna, Sandra; Molins, Laureano; Ramirez, Josep; Monzó, Mariano

    2014-01-01

    Background Several treatments in non-small cell lung cancer (NSCLC) are histology-dependent, and the need for histology-related markers is increasing. MicroRNAs (miRNAs) are promising molecular markers in multiple cancers and show differences in expression depending on histological subtype. The miRNA family miR-200 has been associated with the regulation of epithelial-mesenchymal (EMT)/mesenchymal-epithelial transition (MET). EMT involves profound phenotypic changes that include the loss of cell-cell adhesion, the loss of cell polarity, and the acquisition of migratory and invasive properties that facilitates metastasis. A dual role for the miR-200 family in the prognosis of several tumors has been related to tumor cell origin. However, the prognostic role and function of miR-200 family in early-stage NSCLC adenocarcinoma and squamous cell carcinoma (SCC) have not been well established. Methods miRNA expression was determined using TaqMan assays in 155 tumors from resected NSCLC patients. Functional studies were conducted in three NSCLC cell lines: H23, A-549 and HCC-44. Results High miR-200c expression was associated with shorter overall survival (OS) in the entire cohort (p = 0.024). High miR-200c (p = 0.0004) and miR-141 (p = 0.009) expression correlated with shorter OS in adenocarcinoma – but not in SCC. In the multivariate analysis, a risk score based on miR-141 and miR-200c expression emerged as an independent prognostic factor for OS in the entire cohort (OR, 2.787; p = 0.033) and in adenocarcinoma patients (OR, 10.649; p = 0.002). Functional analyses showed that miR-200c, was related to mesenchymal-epithelial transition (MET) and affected cell migration and E-cadherin levels, while overexpression of miR-141 reduced KLF6 protein levels and produced an increase of secretion of VEGFA in vitro (H23, p = 0.04; A-549, p = 0.03; HCC-44, p = 0.02) and was associated with higher blood microvessel density in patient tumor samples (p

  12. NF-κB-mediated inflammation leading to EMT via miR-200c is involved in cell transformation induced by cigarette smoke extract.

    PubMed

    Zhao, Yue; Xu, Yuan; Li, Yuan; Xu, Wenchao; Luo, Fei; Wang, Bairu; Pang, Ying; Xiang, Quanyong; Zhou, Jianwei; Wang, Xinru; Liu, Qizhan

    2013-10-01

    Cigarette smoking constitutes a major human health hazard because it is the most important risk factor for lung cancer. Although evidence for smoking-induced lung cancer in humans is strong, the molecular mechanisms by which smoking causes cancer remain to be established. In this investigation, we evaluated the roles of inflammation and the epithelial-mesenchymal transition (EMT) in cigarette smoke extract (CSE)-induced transformation of human bronchial epithelial (HBE) cells. The results showed that chronic exposure to CSE induced EMT and transformation of these cells. Activation of nuclear factor-κB (NF-κB) by CSE increased levels of the proinflammatory interleukin-6 (IL-6), and acute and chronic exposures to CSE caused decreases in miR-200c levels. By blocking NF-κB with Bay11-7082 and IL-6 with anti-IL-6 antibody and enhancement of IL-6 with human recombinant IL-6, we found that the NF-κB signal pathway was involved in CSE-induced increases of IL-6, which suppressed miR-200c expression and promoted EMT. Moreover, IL-6 was necessary for maintenance of CSE-induced transformation and for malignant progression of HBE cells. Finally, blocking of NF-κB with Bay11-7082 prevented CSE-induced EMT and malignant transformation due to decreases of E-cadherin and miR-200c and elevations of IL-6, N-cadherin, and vimentin. Thus, we have defined a link between inflammation and EMT, processes involved in the malignant transformation of cells caused by CSE. This link, mediated through miRNAs, establishes a mechanism for CSE-induced lung carcinogenesis. PMID:23824089

  13. Combining TGF-β1 knockdown and miR200c administration to optimize antitumor efficacy of B16F10/GPI-IL-21 vaccine

    PubMed Central

    Wang, Jing; Zhang, Ying; Zhang, Hongyi; Ni, Yaoyao; Sun, Jianan; Wang, Xiaobing; Dou, Jun

    2015-01-01

    TGF-β1 secreted abundantly by tumors cells as well as present in the local microenvironment promotes neoplasm invasion and metastasis by triggering the epithelial to mesenchymal transition (EMT). MiR200c has been shown to suppress EMT and to regulate the cellular epithelial and interstitial state conversion, whereas the tumor vaccines are intended to specifically initiate or amplify a host response against evolving tumor cells. Our study aimed at optimizing the antitumor effects of the B16F10/glycosylphosphatidylinositol-interleukin 21 (B16F10/GPI-IL-21) tumor vaccine on melanoma bearing mice by combining the TGF-β1 knockdown and the administration of miR200c agomir. The mice were subcutaneously vaccinated with inactivated B16F10/GPI-IL-21 vaccine and challenged by B16F10 cells transfected with shTGF-β1 (B16F10/shTGF-β1 cells) or B16F10/shTGF-β1 cells with the administration of miR200c agomir. The later combination showed that, when compared with the mice in the control group that received no vaccination, vaccinated mice significantly increased NK and CTL activities, enhanced levels of IFN-γ, and reduced expression of TGF-β1, N-cadherin, Vimentin, Gli1/2, P-Smad2/3 and others involved in promoting expression of EMT-related molecules in tumor areas, and inhibited the melanoma metastasis in lungs and lymph nodes. Altogether, our findings demonstrate that this synergistic anti-cancer regimen effectively induces strong immune response and diminishes the melanoma progression. PMID:25895132

  14. Integrated Analysis Reveals together miR-182, miR-200c and miR-221 Can Help in the Diagnosis of Prostate Cancer

    PubMed Central

    Qin, Xia; Chen, Panyu; Zou, Yi ming; Hu, Yanling

    2015-01-01

    Research has shown that microRNAs are promising biomarkers that can be used to promote a more accurate diagnosis of cancer. In this study, we developed an integrated multi-step selection process to analyze available high-throughput datasets to obtain information on microRNAs as cancer biomarkers. Applying this approach to the microRNA expression profiles of prostate cancer and the datasets in The Cancer Genome Atlas Data Portal, we identified miRNA-182, miRNA-200c and miRNA-221 as possible biomarkers for prostate cancer. The associations between the expressions of these three microRNAs with clinical parameters as well as their diagnostic capability were studied. Several online databases were used to predict the target genes of these three microRNAs, and the results were confirmed by significant statistical correlations. Comparing with the other 18 types of cancers listed in The Cancer Genome Atlas Data Portal, we found that the combination of both miRNA-182 and miRNA-200c being up-regulated and miRNA-221 being down-regulated only happens in prostate cancer. This provides a unique biological characteristic for prostate cancer that can potentially be used for diagnosis based on tissue testing. In addition, our study also revealed that these three microRNAs are associated with the pathological status of prostate cancer. PMID:26484677

  15. Demethylation drug 5-Aza-2′-deoxycytidine-induced upregulation of miR-200c inhibits the migration, invasion and epithelial-mesenchymal transition of clear cell renal cell carcinoma in vitro

    PubMed Central

    JIANG, JUAN; YI, BO; DING, SIQING; SUN, JIAN; CAO, WEI; LIU, MENGZI

    2016-01-01

    The microRNA (miR)-200 family has been found to be involved in the process of mesenchymal-epithelial transition during renal development. Deregulation of miR-200c has been suggested to be involved in clear cell renal cell carcinoma (ccRCC). However, the precise role of miR-200c in the regulation of ccRCC metastasis has not been previously reported. In the present study, it was observed that miR-200c was frequently downregulated in ccRCC tissue compared with matched adjacent normal tissue. The expression of miR-200c was additionally reduced in ccRCC cell lines when compared with levels in normal renal cells. The DNA demethylation drug 5-Aza-2′-deoxycytidine (Aza) was used to treat several ccRCC cell lines, and it was observed that the expression of miR-200c was significantly increased following Aza treatment. Furthermore, treatment with Aza markedly inhibited ccRCC cell invasion and migration, while treatment with miR-200c inhibitor significantly enhanced invasion and migration of ccRCC cells. In addition, Aza treatment significantly promoted expression of E-cadherin and inhibited the expression of N-cadherin, while the inhibition of miR-200c downregulated E-cadherin and upregulated the expression of N-cadherin, suggesting that miR-200c has a suppressive role in epithelial-mesenchymal transition (EMT) of ccRCC cells. In conclusion, it was suggested that demethylation drug Aza-induced upregulation of miR-200c may inhibit migration, invasion and EMT in ccRCC cells. PMID:27123083

  16. 34 CFR 300.300 - Parental consent.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ....504, obtain informed consent, consistent with § 300.9, from the parent of the child before conducting... efforts to obtain the informed consent from the parent for an initial evaluation to determine whether the... with a disability must obtain informed consent from the parent of the child before the...

  17. 34 CFR 300.300 - Parental consent.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ....504, obtain informed consent, consistent with § 300.9, from the parent of the child before conducting... efforts to obtain the informed consent from the parent for an initial evaluation to determine whether the... with a disability must obtain informed consent from the parent of the child before the...

  18. 34 CFR 300.300 - Parental consent.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ....504, obtain informed consent, consistent with § 300.9, from the parent of the child before conducting... efforts to obtain the informed consent from the parent for an initial evaluation to determine whether the... with a disability must obtain informed consent from the parent of the child before the...

  19. 300 AREA URANIUM CONTAMINATION

    SciTech Connect

    BORGHESE JV

    2009-07-02

    {sm_bullet} Uranium fuel production {sm_bullet} Test reactor and separations experiments {sm_bullet} Animal and radiobiology experiments conducted at the. 331 Laboratory Complex {sm_bullet} .Deactivation, decontamination, decommissioning,. and demolition of 300 Area facilities

  20. p300 family members associate with the carboxyl terminus of simian virus 40 large tumor antigen.

    PubMed Central

    Lill, N L; Tevethia, M J; Eckner, R; Livingston, D M; Modjtahedi, N

    1997-01-01

    Several cellular polypeptides critical for growth regulation interact with DNA tumor virus oncoproteins. p400 is a cellular protein which binds to the adenovirus E1A oncoprotein(s). The biological function of p400 is not yet known, but it is structurally and immunologically closely related to p300 and CREB-binding protein, two known E1A-binding transcription adapters. Like p300, p400 is a phosphoprotein that binds to the simian virus 40 large tumor antigen (T). In anti-T coimmunoprecipitation experiments, staggered deletions spanning the amino-terminal 250 amino acids of T did not abrogate T binding to either p400 or p300. A T species composed of residues 251 to 708 bound both p400 and p300, while a T species defective in p53 binding was unable to bind either detectably. Anti-p53 immunoprecipitates prepared from cells containing wild-type T also contained p400 and p300. Hence, both p400 and p300 can bind (directly or indirectly) to a carboxyl-terminal fragment of T which contains its p53 binding domain. Since the p53 binding domain of T contributes to its immortalizing and transforming activities, T-p400 and/or T-p300 interactions may participate in these functions. PMID:8985331

  1. A spatial, kinematical, and dynamical analysis of Abell 400

    NASA Technical Reports Server (NTRS)

    Beers, Timothy C.; Gebhardt, Karl; Huchra, John P.; Forman, William; Jones, Christine; Bothun, Gregory D.

    1992-01-01

    The paper presents a detailed spatial, kinematical, and dynamical analysis for the cluster A400, based on a nearly complete redshift survey of bright galaxies within 1 Mpc of the cluster center. A dispersed component with a high fraction of spiral galaxies at a velocity of 8200 km/s, and a background group with a mean velocity of 13,400 km/s are identified. It is proposed that the main body of A400 is composed of at least two individual subclusters. If subclustering is ignored, the derived dispersion of the 88 galaxies with measured velocities within 4000 km/s of the bright dumbbell galaxy near the cluster center is 702 km/s. When kinematic information is used to split A400 into likely subclusters, the velocity dispersions of the individual units which make up this cluster are on the order of 200-300 km/s. If A400 is considered a single entity, the inferred blue mass-to-light ratio is 1210 solar masses/solar luminosities. It is argued that A400 is an example of a presently occurring merger, and that the individual components of the dumbbell galaxy were once individual D galaxies within the premerger subclusters.

  2. 76 FR 13534 - Airworthiness Directives; The Boeing Company Model 767-200, -300, -300F, and -400ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-14

    ... proposed AD. Discussion On October 19, 2009, we issued AD 2009-22-13, amendment 39-16066 (74 FR 55755... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3... amendment 39-16066 (74 FR 55755, October 29, 2009) and adding the following new AD: The Boeing...

  3. 76 FR 10288 - Airworthiness Directives; The Boeing Company Model 767-200, -300, -300F, and -400ER Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-02-24

    ... proposed AD. Discussion On September 26, 2005, we issued AD 2004-19-06 R1, amendment 39- 14313 (70 FR 58000... a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26...-06 R1, Amendment 39-14313 (70 FR 58000, October 5, 2005), and adding the following new AD: The...

  4. Physics 300 Provincial Examination.

    ERIC Educational Resources Information Center

    Manitoba Dept. of Education and Training, Winnipeg.

    This document consists of the physics 300 provincial examination (English version), a separate "provincial summary report" on the results of giving the test, and a separate French language version of the examination. This physics examination contains a 53-item multiple choice section and an 12 item free response section. Subsections of the test…

  5. Deutsch 100, 200, 300.

    ERIC Educational Resources Information Center

    Manitoba Dept. of Education, Winnipeg.

    Manitoba's curriculum guide for German 100, 200, and 300 is designed to (1) make teachers of these courses aware of the official policy concerning the nature, goals, and objectives of the courses and approved teaching materials, and (2) provide instructional guidelines. The guide provides information about three sets of teaching materials from…

  6. 76 FR 24349 - Airworthiness Directives; The Boeing Company Model 747-200B, -300, -400, -400D, and -400F Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-02

    ..., 2010 (75 FR 69612). That NPRM proposed to require an inspection to determine the part number of the mid... 12866, (2) Is not a ``significant rule'' under DOT Regulatory Policies and Procedures (44 FR 11034... right access doors of the spring beam mid-pivot bolt assembly for the No. 1 strut were...

  7. 75 FR 69612 - Airworthiness Directives; The Boeing Company Model 747-200B, -300, -400, -400D, and -400F Series...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-15

    ... 12866, 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... bolt assembly. This proposed AD results from a report that the left and right spring beam mid-pivot... bolt assemblies on the spring beam on the outboard struts. Incorrectly installed bolt assemblies...

  8. 34 CFR 300.801-300.802 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false 300.801-300.802 Section 300.801-300.802 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN...

  9. Diagnostic and prognostic relevance of circulating exosomal miR-373, miR-200a, miR-200b and miR-200c in patients with epithelial ovarian cancer

    PubMed Central

    Meng, Xiaodan; Müller, Volkmar; Milde-Langosch, Karin; Trillsch, Fabian; Pantel, Klaus; Schwarzenbach, Heidi

    2016-01-01

    Exosomes are membrane vesicles that mediate intercellular communication by transporting their molecular cargo from cell to cell. We investigated whether serum levels of exosomal miR-373, miR-200a, miR-200b and miR-200c and circulating exosomes have diagnostic and prognostic relevance in a cohort of 163 epithelial ovarian cancer (EOC) patients using TaqMan MicroRNA assays and ELISA. The serum concentrations of exosomal miR-373 (p = 0.0001), miR-200a (p = 0.0001), miR-200b (p = 0.0001) and miR-200c (p = 0.028) were significantly higher in EOC patients than healthy women. The levels of miR-200a (p = 0.0001), miR-200b (p = 0.0001) and miR-200c (p = 0.019) could distinguish between malignant and benign ovarian tumors. While the levels of miR-373 and miR-200a were increased in all FIGO/lymph node stages (p = 0.0001), the levels of miR-200b and miR-200c were higher in patients with FIGO stage III–IV (p = 0.0001, p = 0.008, respectively) including lymph node metastasis (p = 0.0001, p = 0.004, respectively) than FIGO stages I–II. The increased levels of miR-200b and miR-200c were also associated with CA125 values (p = 0.0001, p = 0.0001, respectively) and a shorter overall survival (p = 0.007, p = 0.017, respectively). The levels of exosomes were excessively elevated in EOC patients (p = 0.0001). In all three cohorts, they were positively associated with the serum levels of exosomal miR-373 (p = 0.004), miR-200a (p = 0.0001), miR-200b (p = 0.0001) and miR-200c (p = 0.008). In conclusion, the increased levels of exosomal miR-200b and miR-200c mainly observed in advanced EOC suggest that these microRNAs may be involved in tumor progression. The high concentrations of exosomes in EOC patients imply an excessive, active exosomal secretion in EOC. PMID:26943577

  10. 45 CFR 400.118 - Case planning.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false Case planning. 400.118 Section 400.118 Public... Services § 400.118 Case planning. (a) A State, or its designee under § 400.117, must develop and implement... services. (b) Case planning for unaccompanied minors must, at a minimum, address the following elements:...

  11. 45 CFR 400.118 - Case planning.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true Case planning. 400.118 Section 400.118 Public... Services § 400.118 Case planning. (a) A State, or its designee under § 400.117, must develop and implement... services. (b) Case planning for unaccompanied minors must, at a minimum, address the following elements:...

  12. 45 CFR 400.118 - Case planning.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false Case planning. 400.118 Section 400.118 Public... Services § 400.118 Case planning. (a) A State, or its designee under § 400.117, must develop and implement... services. (b) Case planning for unaccompanied minors must, at a minimum, address the following elements:...

  13. 45 CFR 400.118 - Case planning.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true Case planning. 400.118 Section 400.118 Public... Services § 400.118 Case planning. (a) A State, or its designee under § 400.117, must develop and implement... services. (b) Case planning for unaccompanied minors must, at a minimum, address the following elements:...

  14. Tracker 300 Software

    SciTech Connect

    Wysor, R. Wes

    2006-01-12

    The Tracker300 software is downloaded to an off-the-shelf product called RCM3400/RCM3410 made by Rabbit Semiconductor. The software is a closed loop control which computes the sun's position and provides stability compensation. Using the RCM3400/RCM3410 module, the software stores and retrieves parameters from the onboard flash. The software also allows for communication with a host. It will allow the parameters to be downloaded or uploaded, it will show the status of the controller, it will provide real-time feedback, and it will send command acknowledgements. The software will capture the GPS response and ensure the internal clock is set correctly.

  15. Influence of heat treatment on mechanical properties of 300M steel

    NASA Technical Reports Server (NTRS)

    Youngblood, J. L.; Raghavan, M.

    1975-01-01

    Tests show that 300M steel should be austenitized at temperatures above 1,800 deg. F to yield best combintion of strength and thickness. Tempering should be performed at temperatures between 400 and 600 deg. F

  16. Tracker 300 Software

    2006-01-12

    The Tracker300 software is downloaded to an off-the-shelf product called RCM3400/RCM3410 made by Rabbit Semiconductor. The software is a closed loop control which computes the sun's position and provides stability compensation. Using the RCM3400/RCM3410 module, the software stores and retrieves parameters from the onboard flash. The software also allows for communication with a host. It will allow the parameters to be downloaded or uploaded, it will show the status of the controller, it will providemore » real-time feedback, and it will send command acknowledgements. The software will capture the GPS response and ensure the internal clock is set correctly.« less

  17. 75 FR 906 - Airworthiness Directives; The Boeing Company Model 747-400, -400D, and -400F Series Airplanes

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-07

    ... as of January 22, 2010. On June 12, 2008 (73 FR 25990, May 8, 2008), the Director of the Federal..., Amendment 39-15512 (73 FR 25990, May 8, 2008). That AD applied to certain Model 747-400, - 400D, and -400F... rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3....

  18. Diagnostic arrangement on S-300 facility

    NASA Astrophysics Data System (ADS)

    Bakshaev, Yu. L.; Blinov, P. I.; Chernenko, A. S.; Dan'ko, S. A.; Kalinin, Yu. G.; Korolev, V. D.; Tumanov, V. I.; Shashkov, A. Yu.; Chesnokov, A. V.; Ivanov, M. I.

    2001-01-01

    A set of experiments on neutron and x-ray generation was performed on the S-300 facility for the last few months. The S-300 facility is a pulse generator with electric current achieving 3.5 MA, voltage 400-500 kV, and rise time ˜100 ns. (Chernenko et al., Proc. of the 11th International Conference of Power Particle Beams, Prague, 1966, p. 154). It is designed as van eight-module machine transmitting electromagnetic power to the low-inductive load through the vacuum line with self-magnetic isolation. Diagnostic techniques and methods on S-300 used are described in this article. Ten-channel polychromator is used for the soft x-ray and vacuum ultraviolet bands (50-500 eV) measurements with spectral resolution of 5%-20% and time resolution less than 2.5 ns. Radiated power measurements in the region of 0.1-10 keV are performed by vacuum x-ray diodes and semiconductor detectors equipped with different filters. Curved crystal x-ray spectrography is intended for plasma density, electron, and ion temperature evaluation. Visible and x-ray radiation image converter tubes and a streak camera reproduce plasma dynamics. Laser shadow and schlieren probing are used to investigate rare periphery plasma motion. Time-of-flight and activation technique is designed for total neutron yield determination.

  19. 75 FR 81617 - Determination That TRANDATE (Labetalol Hydrochloride) Tablets, 300 Milligrams and 400 Milligrams...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ..., have never been marketed. In previous instances (see, e.g., 72 FR 9763, March 5, 2007; 61 FR 25497, May...., and initially approved on August 1, 1984. TRANDATE is indicated for the management of...

  20. 40 CFR 81.400 - Scope.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 17 2011-07-01 2011-07-01 false Scope. 81.400 Section 81.400 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF... Service (FS)....

  1. 40 CFR 81.400 - Scope.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 18 2013-07-01 2013-07-01 false Scope. 81.400 Section 81.400 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF... Service (FS)....

  2. 40 CFR 81.400 - Scope.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 18 2012-07-01 2012-07-01 false Scope. 81.400 Section 81.400 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF... Service (FS)....

  3. 40 CFR 81.400 - Scope.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Scope. 81.400 Section 81.400 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) DESIGNATION OF... Service (FS)....

  4. Font Effects of Chinese Characters and Pseudo-Characters on the N400: Evidence for an Orthographic Processing View

    ERIC Educational Resources Information Center

    Lv, Caixia; Wang, Quanhong

    2012-01-01

    Event-related brain potentials (ERPs) were recorded during a Chinese character decision task to examine whether N400 amplitude is modulated by stimulus font. Results revealed large negative-going ERPs in an N400 time window of 300-500 ms to stimuli presented in degraded Xing Kai Ti (XKT) font compared with more intact Song Ti (ST) font regardless…

  5. Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir.

    PubMed

    Wang, Xiaoying; Zhao, Fengshu; Shi, Fangfang; He, Xiangfeng; Pan, Meng; Wu, Di; Li, Miao; Zhang, Yunxia; Dou, Jun

    2016-05-01

    In this study, we hypothesized that the inhibition of epithelial to mesenchymal transition (EMT) program by knockdown of Zinc-finger E-box binding homeobox 1 (ZEB1) or administration of miR200c agomir would strengthen the B16F10 cells transfected with GPI-anchored IL-21 (B16F10/GPI-IL-21) vaccine efficacy in inhibiting the melanoma metastasis. Our findings from the current study indicated that, when compared with the mice immunized with the B16F10/GPI-IL-21 vaccine alone, the mice immunized with B16F10/GPI-IL-21 vaccine combined with injection of shZEB1 plasmid or miR200c agomir not only meaningfully inhibited EMT of melanoma, reduced the EMT characteristic molecular expression in tumor tissues, but also significantly decreased the Treg cells and TGF-β1, enhanced the cytotoxicities of NK cells and cytotoxic T lymphocytes and the IFN-γ level. Furthermore, the immunotherapeutic combination resulted in inhibiting the melanoma growth and lung metastasis. Our study demonstrated that using the B16F10/GPI-IL-21 vaccine in combination with the down-regulated ZEB1 or miR200c administration effectively elicited anti-tumor immunity and reduced melanoma metastasis by inhibiting the EMT program in the B16F10 melanoma-bearing mice. PMID:27133050

  6. 45 CFR 400.317 - Service requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Service requirements. 400.317 Section 400.317... CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Targeted Assistance Funding and Service Priorities § 400.317 Service requirements. In providing targeted...

  7. 15 CFR 400.53 - Information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... information. Disclosure of public information will be governed by 15 CFR part 4. Public information in the... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Information. 400.53 Section 400.53..., Record and Information § 400.53 Information. (a) Request for information. The Board may...

  8. 46 CFR 197.400 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Applicability. 197.400 Section 197.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE OCCUPATIONAL SAFETY AND HEALTH STANDARDS GENERAL PROVISIONS Commercial Diving Operations Operations § 197.400 Applicability. Diving operations...

  9. 46 CFR 197.400 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Applicability. 197.400 Section 197.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE OCCUPATIONAL SAFETY AND HEALTH STANDARDS GENERAL PROVISIONS Commercial Diving Operations Operations § 197.400 Applicability. Diving operations...

  10. 46 CFR 116.400 - Application.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Application. 116.400 Section 116.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING MORE THAN 150... Protection § 116.400 Application. (a) This subpart applies to: (1) Vessels carrying more than 150...

  11. 46 CFR 181.400 - Where required.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Where required. 181.400 Section 181.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Fixed Fire Extinguishing and Detecting Systems § 181.400 Where required. (a) The following spaces must be equipped with a...

  12. 30 CFR 75.400-1 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Definitions. 75.400-1 Section 75.400-1 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Combustible Materials and Rock Dusting § 75.400-1 Definitions....

  13. 30 CFR 75.400-1 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Definitions. 75.400-1 Section 75.400-1 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Combustible Materials and Rock Dusting § 75.400-1 Definitions....

  14. 14 CFR § 1259.400 - Description.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 5 2014-01-01 2014-01-01 false Description. § 1259.400 Section § 1259.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION NATIONAL SPACE GRANT COLLEGE AND FELLOWSHIP PROGRAM Space Grant College and Consortium Designation § 1259.400 Description. (a)...

  15. 14 CFR 1209.400 - Scope.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Scope. 1209.400 Section 1209.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION BOARDS AND COMMITTEES Inventions and Contributions Board § 1209.400 Scope. This subpart describes the functions, authority, and membership of...

  16. 14 CFR 1209.400 - Scope.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Scope. 1209.400 Section 1209.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION BOARDS AND COMMITTEES Inventions and Contributions Board § 1209.400 Scope. This subpart describes the functions, authority, and membership of...

  17. 14 CFR § 1209.400 - Scope.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 5 2014-01-01 2014-01-01 false Scope. § 1209.400 Section § 1209.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION BOARDS AND COMMITTEES Inventions and Contributions Board § 1209.400 Scope. This subpart describes the functions, authority, and membership of...

  18. 14 CFR 1209.400 - Scope.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Scope. 1209.400 Section 1209.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION BOARDS AND COMMITTEES Inventions and Contributions Board § 1209.400 Scope. This subpart describes the functions, authority, and membership of...

  19. 14 CFR 1209.400 - Scope.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Scope. 1209.400 Section 1209.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION BOARDS AND COMMITTEES Inventions and Contributions Board § 1209.400 Scope. This subpart describes the functions, authority, and membership of...

  20. 29 CFR 99.400 - Responsibilities.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 1 2013-07-01 2013-07-01 false Responsibilities. 99.400 Section 99.400 Labor Office of the... Pass-through Entities § 99.400 Responsibilities. (a) Cognizant agency for audit responsibilities... programs of more than one agency. (8) Coordinate the audit work and reporting responsibilities...

  1. 13 CFR 400.204 - Loan terms.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Loan terms. 400.204 Section 400.204 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.204 Loan terms. (a) All loans guaranteed under the Program shall...

  2. 13 CFR 400.204 - Loan terms.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Loan terms. 400.204 Section 400.204 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.204 Loan terms. (a) All loans guaranteed under the Program shall...

  3. 13 CFR 400.204 - Loan terms.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Loan terms. 400.204 Section 400.204 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.204 Loan terms. (a) All loans guaranteed under the Program shall...

  4. 13 CFR 400.207 - Application evaluation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Application evaluation. 400.207 Section 400.207 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.207 Application evaluation. (a) Eligibility...

  5. 13 CFR 400.207 - Application evaluation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Application evaluation. 400.207 Section 400.207 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.207 Application evaluation. (a) Eligibility...

  6. 13 CFR 400.204 - Loan terms.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Loan terms. 400.204 Section 400.204 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.204 Loan terms. (a) All loans guaranteed under the Program shall...

  7. 13 CFR 400.207 - Application evaluation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Application evaluation. 400.207 Section 400.207 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.207 Application evaluation. (a) Eligibility...

  8. 13 CFR 400.207 - Application evaluation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Application evaluation. 400.207 Section 400.207 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.207 Application evaluation. (a) Eligibility...

  9. 13 CFR 400.204 - Loan terms.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Loan terms. 400.204 Section 400.204 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.204 Loan terms. (a) All loans guaranteed under the Program shall...

  10. 33 CFR 143.400 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Applicability. 143.400 Section 143.400 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES DESIGN AND EQUIPMENT Standby Vessels § 143.400 Applicability. This...

  11. 7 CFR 400.132 - Hearings.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false Hearings. 400.132 Section 400.132 Agriculture... Years § 400.132 Hearings. (a) If an employee timely files a petition for a hearing, the FCIC Official will select the date, time, and location for the hearing. (b) The hearing shall be conducted by...

  12. 14 CFR 1259.400 - Description.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Description. 1259.400 Section 1259.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION NATIONAL SPACE GRANT COLLEGE AND FELLOWSHIP PROGRAM Space Grant College and Consortium Designation § 1259.400 Description. (a)...

  13. 14 CFR 1259.400 - Description.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Description. 1259.400 Section 1259.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION NATIONAL SPACE GRANT COLLEGE AND FELLOWSHIP PROGRAM Space Grant College and Consortium Designation § 1259.400 Description. (a)...

  14. 47 CFR 1.400 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Definitions. 1.400 Section 1.400 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL PRACTICE AND PROCEDURE Rulemaking Proceedings General § 1.400 Definitions. As used in this subpart, the term party refers to any person who participates in a proceeding by the timely filing of a...

  15. 7 CFR 400.407 - Restricted access.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false Restricted access. 400.407 Section 400.407 Agriculture... Social Security Account Numbers and Employer Identification Numbers § 400.407 Restricted access. The Manager, other officer, or employee of FCIC or an authorized person may have access to the SSNs and...

  16. 7 CFR 400.407 - Restricted access.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Restricted access. 400.407 Section 400.407 Agriculture... Social Security Account Numbers and Employer Identification Numbers § 400.407 Restricted access. The Manager, other officer, or employee of FCIC or an authorized person may have access to the SSNs and...

  17. 45 CFR 93.400 - Penalties.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Penalties. 93.400 Section 93.400 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL ADMINISTRATION NEW RESTRICTIONS ON LOBBYING Penalties and Enforcement § 93.400 Penalties. (a) Any person who makes an expenditure prohibited herein shall be subject to a civil penalty of not less than...

  18. 45 CFR 400.3 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false 400.3 Section 400.3 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Introduction § 400.3...

  19. 45 CFR 400.3 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true 400.3 Section 400.3 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Introduction § 400.3...

  20. 45 CFR 400.24 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true 400.24 Section 400.24 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration § 400.24...

  1. 45 CFR 400.24 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false 400.24 Section 400.24 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration § 400.24...

  2. 45 CFR 400.26 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false 400.26 Section 400.26 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration § 400.26...

  3. 45 CFR 400.26 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true 400.26 Section 400.26 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration § 400.26...

  4. 15 CFR 400.53 - Information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... information. Disclosure of public information will be governed by 15 CFR part 4. Public information in the... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Information. 400.53 Section 400.53..., Record and Information § 400.53 Information. (a) Request for information. The Board may...

  5. 45 CFR 400.60 - Payment levels.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Payment levels. 400.60 Section 400.60 Public... Assistance § 400.60 Payment levels. (a) Under the public/private RCA program, States and the local... the State's TANF payment for the same sized family unit. In States that have TANF payment levels...

  6. 15 CFR 400.53 - Information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... information. Disclosure of public information will be governed by 15 CFR part 4. Public information in the... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Information. 400.53 Section 400.53..., Record and Information § 400.53 Information. (a) Request for information. The Board may...

  7. 24 CFR 583.400 - Grant agreement.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 3 2011-04-01 2010-04-01 true Grant agreement. 583.400 Section 583.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... DEVELOPMENT COMMUNITY FACILITIES SUPPORTIVE HOUSING PROGRAM Administration § 583.400 Grant agreement....

  8. 24 CFR 583.400 - Grant agreement.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 3 2013-04-01 2013-04-01 false Grant agreement. 583.400 Section 583.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... DEVELOPMENT COMMUNITY FACILITIES SUPPORTIVE HOUSING PROGRAM Administration § 583.400 Grant agreement....

  9. 24 CFR 583.400 - Grant agreement.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Grant agreement. 583.400 Section 583.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... DEVELOPMENT COMMUNITY FACILITIES SUPPORTIVE HOUSING PROGRAM Administration § 583.400 Grant agreement....

  10. 24 CFR 583.400 - Grant agreement.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 3 2014-04-01 2013-04-01 true Grant agreement. 583.400 Section 583.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... DEVELOPMENT COMMUNITY FACILITIES SUPPORTIVE HOUSING PROGRAM Administration § 583.400 Grant agreement....

  11. 47 CFR 68.400 - Content.

    Code of Federal Regulations, 2010 CFR

    2000-10-01

    ... 47 Telecommunication 3 2000-10-01 2000-10-01 false Content. 68.400 Section 68.400 Title 47- FEDERAL COMMUNICATIONS COMMISSION-(CONTINUED) COMMON CARRIER SERVICES (CONTINUED) CONNECTION OF TERMINAL EQUIPMENT TO THE TELEPHONE NETWORK Complaint Procedures § 68.400 Content. A complaint shall be in...

  12. 7 CFR 400.98 - Reconsideration process.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... CFR part 11 before bringing suit against FCIC in a United States district court. However, regardless... 7 Agriculture 6 2010-01-01 2010-01-01 false Reconsideration process. 400.98 Section 400.98..., DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Appeal Procedure § 400.98...

  13. 45 CFR 400.56 - Structure.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false Structure. 400.56 Section 400.56 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND... § 400.56 Structure. (a) States may choose to enter into a partnership agreement with local...

  14. 7 CFR 400.202 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 6 2012-01-01 2012-01-01 false Definitions. 400.202 Section 400.202 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Agency Sales and Service Contract-Standards for Approval § 400.202 Definitions. For the purpose...

  15. 46 CFR 197.400 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Applicability. 197.400 Section 197.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE OCCUPATIONAL SAFETY AND HEALTH STANDARDS GENERAL PROVISIONS Commercial Diving Operations Operations § 197.400 Applicability. Diving operations...

  16. 46 CFR 197.400 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Applicability. 197.400 Section 197.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE OCCUPATIONAL SAFETY AND HEALTH STANDARDS GENERAL PROVISIONS Commercial Diving Operations Operations § 197.400 Applicability. Diving operations...

  17. 46 CFR 197.400 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Applicability. 197.400 Section 197.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE OCCUPATIONAL SAFETY AND HEALTH STANDARDS GENERAL PROVISIONS Commercial Diving Operations Operations § 197.400 Applicability. Diving operations...

  18. 49 CFR 179.400-5 - Materials.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 3 2013-10-01 2013-10-01 false Materials. 179.400-5 Section 179.400-5 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY... Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.400-5 Materials....

  19. 49 CFR 179.400-5 - Materials.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 3 2014-10-01 2014-10-01 false Materials. 179.400-5 Section 179.400-5 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY... Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.400-5 Materials....

  20. 20 CFR 438.400 - Penalties.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Penalties. 438.400 Section 438.400 Employees' Benefits SOCIAL SECURITY ADMINISTRATION RESTRICTIONS ON LOBBYING Penalties and Enforcement § 438.400... effect on the ability of the person to continue in business, any prior violations by the person,...

  1. 49 CFR 179.400-11 - Welding.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 3 2014-10-01 2014-10-01 false Welding. 179.400-11 Section 179.400-11... Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.400-11 Welding. (a... welding procedure, welder, and fabricator must be approved....

  2. 49 CFR 179.400-11 - Welding.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 3 2012-10-01 2012-10-01 false Welding. 179.400-11 Section 179.400-11... Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.400-11 Welding. (a... welding procedure, welder, and fabricator must be approved....

  3. 49 CFR 179.400-11 - Welding.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 3 2011-10-01 2011-10-01 false Welding. 179.400-11 Section 179.400-11... Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.400-11 Welding. (a... welding procedure, welder, and fabricator must be approved....

  4. 49 CFR 179.400-11 - Welding.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 3 2013-10-01 2013-10-01 false Welding. 179.400-11 Section 179.400-11... Liquid Tank Car Tanks and Seamless Steel Tanks (Classes DOT-113 and 107A) § 179.400-11 Welding. (a... welding procedure, welder, and fabricator must be approved....

  5. 47 CFR 68.400 - Content.

    Code of Federal Regulations, 2012 CFR

    1999-10-01

    ... 47 Telecommunication 3 1999-10-01 1999-10-01 false Content. 68.400 Section 68.400 Title 47- FEDERAL COMMUNICATIONS COMMISSION-(CONTINUED) COMMON CARRIER SERVICES (CONTINUED) CONNECTION OF TERMINAL EQUIPMENT TO THE TELEPHONE NETWORK Complaint Procedures § 68.400 Content. A complaint shall be in...

  6. 47 CFR 68.400 - Content.

    Code of Federal Regulations, 2013 CFR

    1997-10-01

    ... 47 Telecommunication 3 1997-10-01 1997-10-01 false Content. 68.400 Section 68.400 COMMON CARRIER SERVICES (CONTINUED) CONNECTION OF TERMINAL EQUIPMENT TO THE TELEPHONE NETWORK Complaint Procedures § 68.400 Content. A complaint shall be in writing and shall contain: (a) The name and address of...

  7. 47 CFR 68.400 - Content.

    Code of Federal Regulations, 2014 CFR

    1998-10-01

    ... 47 Telecommunication 3 1998-10-01 1998-10-01 false Content. 68.400 Section 68.400 COMMON CARRIER SERVICES (CONTINUED) CONNECTION OF TERMINAL EQUIPMENT TO THE TELEPHONE NETWORK Complaint Procedures § 68.400 Content. A complaint shall be in writing and shall contain: (a) The name and address of...

  8. 47 CFR 68.400 - Content.

    Code of Federal Regulations, 2012 CFR

    1996-10-01

    ... 47 Telecommunication 3 1996-10-01 1996-10-01 false Content. 68.400 Section 68.400 COMMON CARRIER SERVICES (CONTINUED) CONNECTION OF TERMINAL EQUIPMENT TO THE TELEPHONE NETWORK Complaint Procedures § 68.400 Content. A complaint shall be in writing and shall contain: (a) The name and address of...

  9. 45 CFR 400.13 - Cost allocation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Cost allocation. 400.13 Section 400.13 Public... for Refugee Resettlement Award of Grants to States § 400.13 Cost allocation. (a) A State must allocate... management of the State's refugee program (e.g., development of the State plan, overall program...

  10. 45 CFR 400.13 - Cost allocation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false Cost allocation. 400.13 Section 400.13 Public... for Refugee Resettlement Award of Grants to States § 400.13 Cost allocation. (a) A State must allocate... management of the State's refugee program (e.g., development of the State plan, overall program...

  11. 46 CFR 116.400 - Application.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Application. 116.400 Section 116.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING MORE THAN 150... Protection § 116.400 Application. (a) This subpart applies to: (1) Vessels carrying more than 150...

  12. 29 CFR 99.400 - Responsibilities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 1 2012-07-01 2012-07-01 false Responsibilities. 99.400 Section 99.400 Labor Office of the... Pass-through Entities § 99.400 Responsibilities. (a) Cognizant agency for audit responsibilities... programs of more than one agency. (8) Coordinate the audit work and reporting responsibilities...

  13. 29 CFR 99.400 - Responsibilities.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 1 2011-07-01 2011-07-01 false Responsibilities. 99.400 Section 99.400 Labor Office of the... Pass-through Entities § 99.400 Responsibilities. (a) Cognizant agency for audit responsibilities... programs of more than one agency. (8) Coordinate the audit work and reporting responsibilities...

  14. 47 CFR 400.1 - Purpose.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 5 2014-10-01 2014-10-01 false Purpose. 400.1 Section 400.1 Telecommunication NATIONAL TELECOMMUNICATIONS AND INFORMATION ADMINISTRATION, DEPARTMENT OF COMMERCE, AND NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION E-911 GRANT PROGRAM § 400.1 Purpose. This...

  15. 47 CFR 400.1 - Purpose.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 5 2012-10-01 2012-10-01 false Purpose. 400.1 Section 400.1 Telecommunication NATIONAL TELECOMMUNICATIONS AND INFORMATION ADMINISTRATION, DEPARTMENT OF COMMERCE, AND NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION E-911 GRANT PROGRAM § 400.1 Purpose. This...

  16. 47 CFR 400.1 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 5 2011-10-01 2011-10-01 false Purpose. 400.1 Section 400.1 Telecommunication NATIONAL TELECOMMUNICATIONS AND INFORMATION ADMINISTRATION, DEPARTMENT OF COMMERCE, AND NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION E-911 GRANT PROGRAM § 400.1 Purpose. This...

  17. 47 CFR 400.1 - Purpose.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 5 2013-10-01 2013-10-01 false Purpose. 400.1 Section 400.1 Telecommunication NATIONAL TELECOMMUNICATIONS AND INFORMATION ADMINISTRATION, DEPARTMENT OF COMMERCE, AND NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION E-911 GRANT PROGRAM § 400.1 Purpose. This...

  18. 47 CFR 400.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Purpose. 400.1 Section 400.1 Telecommunication NATIONAL TELECOMMUNICATIONS AND INFORMATION ADMINISTRATION, DEPARTMENT OF COMMERCE, AND NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION E-911 GRANT PROGRAM § 400.1 Purpose. This...

  19. 7 CFR 400.127 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false 400.127 Section 400.127 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Debt Management-Regulations for the 1986 and Succeeding Crop Years § 400.127...

  20. 45 CFR 400.24 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false 400.24 Section 400.24 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration § 400.24...

  1. 45 CFR 400.26 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false 400.26 Section 400.26 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration § 400.26...

  2. 45 CFR 400.3 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false 400.3 Section 400.3 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Introduction § 400.3...

  3. 45 CFR 400.155 - Other services.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true Other services. 400.155 Section 400.155 Public... AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee Social Services Scope of Refugee Social Services § 400.155 Other services. A State may provide the following...

  4. 45 CFR 400.106 - Additional services.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true Additional services. 400.106 Section 400.106 Public... AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee Medical Assistance Scope of Medical Services § 400.106 Additional services. If a State or local jurisdiction...

  5. 45 CFR 400.317 - Service requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true Service requirements. 400.317 Section 400.317... CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Targeted Assistance Funding and Service Priorities § 400.317 Service requirements. In providing targeted...

  6. 7 CFR 400.170 - General qualifications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false General qualifications. 400.170 Section 400.170..., DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Reinsurance Agreement-Standards for Approval; Regulations for the 1997 and Subsequent Reinsurance Years § 400.170 General qualifications. To...

  7. 7 CFR 400.170 - General qualifications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false General qualifications. 400.170 Section 400.170..., DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Reinsurance Agreement-Standards for Approval; Regulations for the 1997 and Subsequent Reinsurance Years § 400.170 General qualifications. To...

  8. 13 CFR 400.105 - Staff.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Staff. 400.105 Section 400.105... Board Procedures § 400.105 Staff. (a) Executive Director. The Executive Director of the Board advises... with respect to the administration of the Board's actions, directs the activities of the staff,...

  9. 7 CFR 400.57 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 6 2014-01-01 2014-01-01 false 400.57 Section 400.57 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Actual Production History § 400.57...

  10. 7 CFR 400.57 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 6 2012-01-01 2012-01-01 false 400.57 Section 400.57 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Actual Production History § 400.57...

  11. 7 CFR 400.57 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 6 2013-01-01 2013-01-01 false 400.57 Section 400.57 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Actual Production History § 400.57...

  12. 45 CFR 400.111 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Definitions. 400.111 Section 400.111 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN... Services § 400.111 Definitions. For purposes of this subpart— Child welfare agency means an agency...

  13. 45 CFR 400.26 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true 400.26 Section 400.26 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration § 400.26...

  14. 45 CFR 400.3 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false 400.3 Section 400.3 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Introduction § 400.3...

  15. 45 CFR 400.24 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false 400.24 Section 400.24 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration § 400.24...

  16. 45 CFR 400.114 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false 400.114 Section 400.114 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Child Welfare Services § 400.114...

  17. 45 CFR 400.24 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true 400.24 Section 400.24 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration § 400.24...

  18. 45 CFR 400.3 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true 400.3 Section 400.3 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Introduction § 400.3...

  19. 45 CFR 400.26 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false 400.26 Section 400.26 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration § 400.26...

  20. 13 CFR 400.205 - Application process.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Application process. 400.205 Section 400.205 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.205 Application process. (a) Application process....

  1. 13 CFR 400.205 - Application process.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Application process. 400.205 Section 400.205 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.205 Application process. (a) Application process....

  2. 45 CFR 400.107 - Medical screening.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false Medical screening. 400.107 Section 400.107 Public... AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee Medical Assistance Scope of Medical Services § 400.107 Medical screening. (a) As part of its refugee...

  3. 45 CFR 400.107 - Medical screening.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true Medical screening. 400.107 Section 400.107 Public... AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee Medical Assistance Scope of Medical Services § 400.107 Medical screening. (a) As part of its refugee...

  4. 45 CFR 400.107 - Medical screening.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false Medical screening. 400.107 Section 400.107 Public... AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee Medical Assistance Scope of Medical Services § 400.107 Medical screening. (a) As part of its refugee...

  5. 45 CFR 400.107 - Medical screening.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Medical screening. 400.107 Section 400.107 Public... AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee Medical Assistance Scope of Medical Services § 400.107 Medical screening. (a) As part of its refugee...

  6. 45 CFR 400.107 - Medical screening.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true Medical screening. 400.107 Section 400.107 Public... AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee Medical Assistance Scope of Medical Services § 400.107 Medical screening. (a) As part of its refugee...

  7. 40 CFR 272.400 - State authorization.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 27 2014-07-01 2014-07-01 false State authorization. 272.400 Section 272.400 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) APPROVED STATE HAZARDOUS WASTE MANAGEMENT PROGRAMS Delaware § 272.400 State authorization. (a) The State of Delaware is authorized to administer...

  8. 45 CFR 400.56 - Structure.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Structure. 400.56 Section 400.56 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND... § 400.56 Structure. (a) States may choose to enter into a partnership agreement with local...

  9. 45 CFR 400.56 - Structure.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false Structure. 400.56 Section 400.56 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND... § 400.56 Structure. (a) States may choose to enter into a partnership agreement with local...

  10. 45 CFR 400.317 - Service requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false Service requirements. 400.317 Section 400.317... CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Targeted Assistance Funding and Service Priorities § 400.317 Service requirements. In providing targeted...

  11. 45 CFR 400.60 - Payment levels.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false Payment levels. 400.60 Section 400.60 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee Cash Assistance § 400.60 Payment levels. (a) Under...

  12. 46 CFR 181.400 - Where required.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Where required. 181.400 Section 181.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Fixed Fire Extinguishing and Detecting Systems § 181.400 Where required. (a) The following spaces must be equipped with a...

  13. 7 CFR 400.57 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false 400.57 Section 400.57 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Actual Production History § 400.57...

  14. 7 CFR 400.57 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false 400.57 Section 400.57 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Actual Production History § 400.57...

  15. 17 CFR 200.400 - Open meetings.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Open meetings. 200.400 Section 200.400 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION ORGANIZATION; CONDUCT... Meetings § 200.400 Open meetings. Except as otherwise provided in this subpart, meetings of the...

  16. 30 CFR 75.400-1 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Definitions. 75.400-1 Section 75.400-1 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Combustible Materials and Rock Dusting § 75.400-1 Definitions....

  17. 30 CFR 75.400-1 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Definitions. 75.400-1 Section 75.400-1 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Combustible Materials and Rock Dusting § 75.400-1 Definitions....

  18. 30 CFR 75.400-1 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Definitions. 75.400-1 Section 75.400-1 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Combustible Materials and Rock Dusting § 75.400-1 Definitions....

  19. 7 CFR 3018.400 - Penalties.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false Penalties. 3018.400 Section 3018.400 Agriculture Regulations of the Department of Agriculture (Continued) OFFICE OF THE CHIEF FINANCIAL OFFICER, DEPARTMENT OF AGRICULTURE NEW RESTRICTIONS ON LOBBYING Penalties and Enforcement § 3018.400 Penalties. (a) Any person...

  20. 47 CFR 400.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false Definitions. 400.2 Section 400.2 Telecommunication NATIONAL TELECOMMUNICATIONS AND INFORMATION ADMINISTRATION, DEPARTMENT OF COMMERCE, AND NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION E-911 GRANT PROGRAM § 400.2 Definitions. As used in this part—...

  1. 46 CFR 130.400 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Applicability. 130.400 Section 130.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS VESSEL CONTROL, AND MISCELLANEOUS EQUIPMENT AND SYSTEMS Automation of Unattended Machinery Spaces § 130.400 Applicability. Except...

  2. 46 CFR 130.400 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Applicability. 130.400 Section 130.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS VESSEL CONTROL, AND MISCELLANEOUS EQUIPMENT AND SYSTEMS Automation of Unattended Machinery Spaces § 130.400 Applicability....

  3. 46 CFR 130.400 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Applicability. 130.400 Section 130.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS VESSEL CONTROL, AND MISCELLANEOUS EQUIPMENT AND SYSTEMS Automation of Unattended Machinery Spaces § 130.400 Applicability....

  4. 46 CFR 130.400 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Applicability. 130.400 Section 130.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS VESSEL CONTROL, AND MISCELLANEOUS EQUIPMENT AND SYSTEMS Automation of Unattended Machinery Spaces § 130.400 Applicability....

  5. 46 CFR 130.400 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Applicability. 130.400 Section 130.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OFFSHORE SUPPLY VESSELS VESSEL CONTROL, AND MISCELLANEOUS EQUIPMENT AND SYSTEMS Automation of Unattended Machinery Spaces § 130.400 Applicability....

  6. 45 CFR 400.13 - Cost allocation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true Cost allocation. 400.13 Section 400.13 Public... for Refugee Resettlement Award of Grants to States § 400.13 Cost allocation. (a) A State must allocate... management of the State's refugee program (e.g., development of the State plan, overall program...

  7. 45 CFR 400.13 - Cost allocation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false Cost allocation. 400.13 Section 400.13 Public... for Refugee Resettlement Award of Grants to States § 400.13 Cost allocation. (a) A State must allocate... management of the State's refugee program (e.g., development of the State plan, overall program...

  8. 15 CFR 400.43 - Uniform treatment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Uniform treatment. 400.43 Section 400... Zones and Administrative Requirements § 400.43 Uniform treatment. Pursuant to Section 14 of the FTZ Act... uniform treatment under like conditions. Treatment of zone participants within a zone...

  9. 15 CFR 400.43 - Uniform treatment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Uniform treatment. 400.43 Section 400... Zones and Administrative Requirements § 400.43 Uniform treatment. Pursuant to Section 14 of the FTZ Act... uniform treatment under like conditions. Treatment of zone participants within a zone...

  10. 29 CFR 99.400 - Responsibilities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 1 2014-07-01 2013-07-01 true Responsibilities. 99.400 Section 99.400 Labor Office of the... Pass-through Entities § 99.400 Responsibilities. (a) Cognizant agency for audit responsibilities... programs of more than one agency. (8) Coordinate the audit work and reporting responsibilities...

  11. 29 CFR 1926.400 - Introduction.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 8 2010-07-01 2010-07-01 false Introduction. 1926.400 Section 1926.400 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR CONSTRUCTION Electrical General § 1926.400 Introduction. This...

  12. 14 CFR 400.2 - Scope.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... not apply to amateur rockets activities, as defined in 14 CFR 1.1, or to space activities carried out... 14 Aeronautics and Space 4 2011-01-01 2011-01-01 false Scope. 400.2 Section 400.2 Aeronautics and... GENERAL BASIS AND SCOPE § 400.2 Scope. These regulations set forth the procedures and...

  13. 14 CFR 400.2 - Scope.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... not apply to amateur rockets activities, as defined in 14 CFR 1.1, or to space activities carried out... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Scope. 400.2 Section 400.2 Aeronautics and... GENERAL BASIS AND SCOPE § 400.2 Scope. These regulations set forth the procedures and...

  14. Ultradeep (greater than 300 kilometers), ultramafic upper mantle xenoliths.

    PubMed

    Haggerty, S E; Sautter, V

    1990-05-25

    Geophysical discontinuities in Earth's upper mantle and experimental data predict the structural transformation of pyroxene to garnet and the solid-state dissolution of pyroxene into garnet with increasing depth. These predictions are indirectly verified by omphacitic pyroxene exsolution in pyropic garnet-bearing xenoliths from a diamondiferous kimberlite. Conditions for silicon in octahedral sites in the original garnets are met at pressures greater than 130 kilobars, placing the origin of these xenoliths at depths of 300 to 400 kilometers. These ultradeep xenoliths support the theory that the 400-km seismic discontinuity is marked by a transition from peridotite to eclogite. PMID:17745405

  15. 300 Junior Novel Anticipation Guides

    ERIC Educational Resources Information Center

    Polette, Nancy

    2006-01-01

    In this book, the author has created reproducible anticipation guides to 300 popular junior novels (grades 4-8) ranging from classics like Ben and Me and Cricket in Times Square to the current award winner, Kira, Kira. The book consists of 300 reproducible sets of open ended values questions to pique the interest of readers. Librarians and…

  16. 34 CFR 82.400 - Penalties.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false Penalties. 82.400 Section 82.400 Education Office of the Secretary, Department of Education NEW RESTRICTIONS ON LOBBYING Penalties and Enforcement § 82.400 Penalties. (a) Any person who makes an expenditure prohibited herein shall be subject to a civil penalty of not less than $10,000 and not more...

  17. A Lexical Basis for N400 Context Effects: Evidence from MEG

    ERIC Educational Resources Information Center

    Lau, Ellen; Almeida, Diogo; Hines, Paul C.; Poeppel, David

    2009-01-01

    The electrophysiological response to words during the "N400" time window (approximately 300-500 ms post-onset) is affected by the context in which the word is presented, but whether this effect reflects the impact of context on "access" of the stored lexical information itself or, alternatively, post-access "integration" processes is still an open…

  18. Operational status of TAMA300

    NASA Astrophysics Data System (ADS)

    Takahashi, Ryutaro; TAMA Collaboration

    2003-09-01

    The project of a laser interferometer gravitational wave detector TAMA300 is in progress. The 300 m Fabry Perot Michelson interferometer was successfully locked with power recycling in December 2001. The sensitivity was improved owing to further frequency stabilization as well as additional power gain. The seventh data taking run was held as the first international coincidence run with Laser Interferometer Gravitational Wave Observatory (LIGO) and GEO600. Duty cycle of TAMA300 was 76.7%. Five detectors were under the coincidence observation for 9.8 h during the 34 h run.

  19. 24 CFR 570.400 - General.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... contained in 24 CFR part 58 also apply to this subpart, unless otherwise specifically provided herein. (b... 24 Housing and Urban Development 3 2011-04-01 2010-04-01 true General. 570.400 Section 570.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE...

  20. 24 CFR 200.400 - Purpose.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Purpose. 200.400 Section 200.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN...

  1. 24 CFR 572.400 - Consolidated plan.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Requirements § 572.400 Consolidated plan. Applicants must provide a certification of consistency with the approved consolidated plan, in accordance with 24 CFR 91.510. ... 24 Housing and Urban Development 3 2013-04-01 2013-04-01 false Consolidated plan. 572.400...

  2. 24 CFR 572.400 - Consolidated plan.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Requirements § 572.400 Consolidated plan. Applicants must provide a certification of consistency with the approved consolidated plan, in accordance with 24 CFR 91.510. ... 24 Housing and Urban Development 3 2014-04-01 2013-04-01 true Consolidated plan. 572.400...

  3. 13 CFR 400.203 - Guarantee percentage.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Section 400.203 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.203 Guarantee percentage. A guarantee issued by the Board may not exceed 85 percent of the amount of the principal of a loan to a Qualified Steel...

  4. 13 CFR 400.203 - Guarantee percentage.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Section 400.203 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.203 Guarantee percentage. A guarantee issued by the Board may not exceed 85 percent of the amount of the principal of a loan to a Qualified Steel...

  5. 13 CFR 400.200 - Eligible Borrower.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 400.200 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.200 Eligible Borrower. (a) An eligible Borrower must be a Qualified Steel Company that can demonstrate: (1) Credit is not otherwise available to it under...

  6. 13 CFR 400.203 - Guarantee percentage.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Section 400.203 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.203 Guarantee percentage. A guarantee issued by the Board may not exceed 85 percent of the amount of the principal of a loan to a Qualified Steel...

  7. 13 CFR 400.203 - Guarantee percentage.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Section 400.203 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.203 Guarantee percentage. A guarantee issued by the Board may not exceed 85 percent of the amount of the principal of a loan to a Qualified Steel...

  8. 13 CFR 400.205 - Application process.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Section 400.205 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.205 Application process. (a) Application process. An... “Application for Steel Guarantee Loan”; (2) The information required for the completion of Form...

  9. 13 CFR 400.205 - Application process.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Section 400.205 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.205 Application process. (a) Application process. An... “Application for Steel Guarantee Loan”; (2) The information required for the completion of Form...

  10. 13 CFR 400.201 - Eligible Lender.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 400.201 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.201 Eligible Lender. (a) A lender eligible to apply to the...), including its experience with loans to steel companies; (3) The scope, volume and duration of the...

  11. 13 CFR 400.201 - Eligible Lender.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 400.201 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.201 Eligible Lender. (a) A lender eligible to apply to the...), including its experience with loans to steel companies; (3) The scope, volume and duration of the...

  12. 13 CFR 400.201 - Eligible Lender.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 400.201 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.201 Eligible Lender. (a) A lender eligible to apply to the...), including its experience with loans to steel companies; (3) The scope, volume and duration of the...

  13. 13 CFR 400.200 - Eligible Borrower.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 400.200 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.200 Eligible Borrower. (a) An eligible Borrower must be a Qualified Steel Company that can demonstrate: (1) Credit is not otherwise available to it under...

  14. 13 CFR 400.203 - Guarantee percentage.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Section 400.203 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.203 Guarantee percentage. A guarantee issued by the Board may not exceed 85 percent of the amount of the principal of a loan to a Qualified Steel...

  15. 13 CFR 400.201 - Eligible Lender.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 400.201 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.201 Eligible Lender. (a) A lender eligible to apply to the...), including its experience with loans to steel companies; (3) The scope, volume and duration of the...

  16. 13 CFR 400.205 - Application process.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Section 400.205 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.205 Application process. (a) Application process. An... “Application for Steel Guarantee Loan”; (2) The information required for the completion of Form...

  17. 13 CFR 400.201 - Eligible Lender.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 400.201 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.201 Eligible Lender. (a) A lender eligible to apply to the...), including its experience with loans to steel companies; (3) The scope, volume and duration of the...

  18. 13 CFR 400.200 - Eligible Borrower.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 400.200 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.200 Eligible Borrower. (a) An eligible Borrower must be a Qualified Steel Company that can demonstrate: (1) Credit is not otherwise available to it under...

  19. 13 CFR 400.200 - Eligible Borrower.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 400.200 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.200 Eligible Borrower. (a) An eligible Borrower must be a Qualified Steel Company that can demonstrate: (1) Credit is not otherwise available to it under...

  20. 13 CFR 400.200 - Eligible Borrower.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 400.200 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.200 Eligible Borrower. (a) An eligible Borrower must be a Qualified Steel Company that can demonstrate: (1) Credit is not otherwise available to it under...

  1. 7 CFR 400.161 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false Definitions. 400.161 Section 400.161 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Reinsurance Agreement-Standards for Approval; Regulations for the 1997 and Subsequent...

  2. 45 CFR 400.23 - Hearings.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Hearings. 400.23 Section 400.23 Public Welfare....23 Hearings. (a) A State must provide applicants for, and recipients of, assistance and services under the Act with an opportunity for a hearing to contest adverse determinations using...

  3. 45 CFR 400.23 - Hearings.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false Hearings. 400.23 Section 400.23 Public Welfare....23 Hearings. (a) A State must provide applicants for, and recipients of, assistance and services under the Act with an opportunity for a hearing to contest adverse determinations using...

  4. 14 CFR 1206.400 - Information Centers.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Information Centers. 1206.400 Section 1206.400 Aeronautics and Space NATIONAL AERONAUTICS AND SPACE ADMINISTRATION AVAILABILITY OF AGENCY RECORDS... Centers. NASA will maintain Information Centers as set forth in this subpart....

  5. 45 CFR 604.400 - Penalties.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Penalties. 604.400 Section 604.400 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION NEW RESTRICTIONS ON LOBBYING... of any such penalty, by reason of a violation by any person, the agency shall consider the...

  6. 45 CFR 604.400 - Penalties.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 3 2012-10-01 2012-10-01 false Penalties. 604.400 Section 604.400 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION NEW RESTRICTIONS ON LOBBYING... of any such penalty, by reason of a violation by any person, the agency shall consider the...

  7. 45 CFR 604.400 - Penalties.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Penalties. 604.400 Section 604.400 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION NEW RESTRICTIONS ON LOBBYING... of any such penalty, by reason of a violation by any person, the agency shall consider the...

  8. 45 CFR 604.400 - Penalties.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 3 2013-10-01 2013-10-01 false Penalties. 604.400 Section 604.400 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION NEW RESTRICTIONS ON LOBBYING... of any such penalty, by reason of a violation by any person, the agency shall consider the...

  9. 45 CFR 604.400 - Penalties.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Penalties. 604.400 Section 604.400 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL SCIENCE FOUNDATION NEW RESTRICTIONS ON LOBBYING... of any such penalty, by reason of a violation by any person, the agency shall consider the...

  10. 14 CFR 1252.400 - Compliance reviews.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Compliance reviews. 1252.400 Section 1252..., and Enforcement Procedures § 1252.400 Compliance reviews. (a) NASA may conduct compliance reviews and pre-award reviews of recipients or use other similar procedures that will permit it to investigate...

  11. 17 CFR 201.400 - Interlocutory review.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 2 2010-04-01 2010-04-01 false Interlocutory review. 201.400... Rules of Practice Appeal to the Commission and Commission Review § 201.400 Interlocutory review. (a... review. Petitions by parties for interlocutory review are disfavored, and the Commission ordinarily...

  12. 15 CFR 400.29 - Application fees.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...) Special-purpose subzones (§ 400.25): (i) Non-manufacturing/processing or less than three products 4,000 (ii) Manufacturing/processing—three or more products 6,500 (3) Expansions (§ 400.26(b)) 1,600...

  13. 45 CFR 400.6 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true 400.6 Section 400.6 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Grants to States for...

  14. 45 CFR 400.6 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false 400.6 Section 400.6 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Grants to States for...

  15. 45 CFR 400.25 - Residency requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true Residency requirements. 400.25 Section 400.25 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM...

  16. 45 CFR 400.23 - Hearings.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true Hearings. 400.23 Section 400.23 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration §...

  17. 45 CFR 400.10 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 2 2014-10-01 2012-10-01 true 400.10 Section 400.10 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Grants to States for...

  18. 45 CFR 400.25 - Residency requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false Residency requirements. 400.25 Section 400.25 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM...

  19. 45 CFR 400.10 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 2 2011-10-01 2011-10-01 false 400.10 Section 400.10 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Grants to States for...

  20. 45 CFR 400.155 - Other services.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Other services. 400.155 Section 400.155 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee Social Services Scope of Refugee Social Services...

  1. 7 CFR 400.404 - Policyholder responsibilities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Policyholder responsibilities. 400.404 Section 400.404 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS General Administrative Regulations; Collection and Storage of Social...

  2. 24 CFR 583.400 - Grant agreement.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 3 2012-04-01 2012-04-01 false Grant agreement. 583.400 Section 583.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT COMMUNITY FACILITIES...

  3. 24 CFR 582.400 - Grant agreement.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 3 2010-04-01 2010-04-01 false Grant agreement. 582.400 Section 582.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND...

  4. 24 CFR 582.400 - Grant agreement.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 3 2013-04-01 2013-04-01 false Grant agreement. 582.400 Section 582.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND...

  5. 24 CFR 582.400 - Grant agreement.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 3 2011-04-01 2010-04-01 true Grant agreement. 582.400 Section 582.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND...

  6. 24 CFR 582.400 - Grant agreement.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 3 2012-04-01 2012-04-01 false Grant agreement. 582.400 Section 582.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT COMMUNITY FACILITIES SHELTER...

  7. 24 CFR 582.400 - Grant agreement.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 3 2014-04-01 2013-04-01 true Grant agreement. 582.400 Section 582.400 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR COMMUNITY PLANNING AND DEVELOPMENT, DEPARTMENT OF HOUSING AND...

  8. 45 CFR 400.118 - Case planning.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Child Welfare Services § 400.118 Case planning. (a) A State, or its designee under § 400.117, must develop and...

  9. 13 CFR 400.207 - Application evaluation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... section shall be subject to a substantive review by the Board based upon the following evaluation factors... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Application evaluation. 400.207... GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.207 Application evaluation. (a) Eligibility...

  10. 21 CFR 556.400 - Methylprednisolone.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 6 2010-04-01 2010-04-01 false Methylprednisolone. 556.400 Section 556.400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) ANIMAL DRUGS, FEEDS, AND RELATED PRODUCTS TOLERANCES FOR RESIDUES OF NEW ANIMAL DRUGS IN FOOD Specific Tolerances for Residues of New Animal Drugs §...

  11. 15 CFR 400.44 - Zone schedule.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Zone schedule. 400.44 Section 400.44 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Operation of Zones...

  12. 15 CFR 400.29 - Application fees.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Application fees. 400.29 Section 400.29 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Applications...

  13. 15 CFR 400.29 - Application fees.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Application fees. 400.29 Section 400.29 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Establishment...

  14. 15 CFR 400.22 - Eligible applicants.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Eligible applicants. 400.22 Section 400.22 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES...

  15. 15 CFR 400.22 - Eligible applicants.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Eligible applicants. 400.22 Section 400.22 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES...

  16. 15 CFR 400.6 - Board headquarters.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Board headquarters. 400.6 Section 400.6 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD...

  17. 15 CFR 400.1 - Scope.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Scope. 400.1 Section 400.1 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Scope, Definitions...

  18. 15 CFR 400.12 - Eligible applicants.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Eligible applicants. 400.12 Section 400.12 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Ability...

  19. 15 CFR 400.13 - Board headquarters.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Board headquarters. 400.13 Section 400.13 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD...

  20. 15 CFR 400.1 - Scope.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Scope. 400.1 Section 400.1 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Scope and Definitions §...

  1. 15 CFR 400.2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Definitions. 400.2 Section 400.2 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Scope and...

  2. 15 CFR 400.13 - Board headquarters.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Board headquarters. 400.13 Section 400.13 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD...

  3. 15 CFR 400.15 - Production equipment.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Production equipment. 400.15 Section 400.15 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Ability...

  4. 15 CFR 400.46 - Grantee liability.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Grantee liability. 400.46 Section 400.46 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Operation...

  5. 15 CFR 400.29 - Application fees.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Application fees. 400.29 Section 400.29 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Establishment...

  6. 15 CFR 400.13 - Board headquarters.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Board headquarters. 400.13 Section 400.13 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD...

  7. 15 CFR 400.1 - Scope.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Scope. 400.1 Section 400.1 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Scope, Definitions...

  8. 15 CFR 400.29 - Application fees.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Application fees. 400.29 Section 400.29 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Applications...

  9. 15 CFR 400.22 - Eligible applicants.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Eligible applicants. 400.22 Section 400.22 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES...

  10. 15 CFR 400.6 - Board headquarters.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Board headquarters. 400.6 Section 400.6 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD...

  11. 15 CFR 400.44 - Zone schedule.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Zone schedule. 400.44 Section 400.44 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Operation of Zones...

  12. 15 CFR 400.46 - Grantee liability.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Grantee liability. 400.46 Section 400.46 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Operation...

  13. 15 CFR 400.2 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Definitions. 400.2 Section 400.2 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Scope and...

  14. 15 CFR 400.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Definitions. 400.2 Section 400.2 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Scope and...

  15. 15 CFR 400.12 - Eligible applicants.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Eligible applicants. 400.12 Section 400.12 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Ability...

  16. 7 CFR 400.402 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false Definitions. 400.402 Section 400.402 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS General Administrative Regulations; Collection and Storage of Social Security Account Numbers...

  17. 7 CFR 400.402 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 6 2012-01-01 2012-01-01 false Definitions. 400.402 Section 400.402 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS General Administrative Regulations; Collection and Storage of Social Security Account Numbers...

  18. 7 CFR 400.302 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false Definitions. 400.302 Section 400.302 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Non-Standard Underwriting Classification System Regulations for the 1991 and Succeeding Crop Years...

  19. 49 CFR 179.400-24 - Stamping.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Stamping. 179.400-24 Section 179.400-24 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS SPECIFICATIONS FOR TANK CARS Specification for Cryogenic Liquid Tank Car Tanks...

  20. 29 CFR 780.400 - Statutory provisions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 3 2014-07-01 2014-07-01 false Statutory provisions. 780.400 Section 780.400 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR STATEMENTS OF GENERAL POLICY OR INTERPRETATION NOT DIRECTLY RELATED TO REGULATIONS EXEMPTIONS APPLICABLE TO AGRICULTURE, PROCESSING OF AGRICULTURAL COMMODITIES,...

  1. 15 CFR 400.1 - Scope.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... regulations of the United States Customs Service (19 CFR part 146) governs zone operations, including the... merchandise from zones. (c) To the extent “activated” under Customs procedures in 19 CFR part 146, and only... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Scope. 400.1 Section 400.1...

  2. 49 CFR 179.400-5 - Materials.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Materials. 179.400-5 Section 179.400-5 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS SPECIFICATIONS FOR TANK...

  3. 49 CFR 179.400-11 - Welding.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Welding. 179.400-11 Section 179.400-11...-11 Welding. (a) Except for closure of openings and a maximum of two circumferential closing joints in... subchapter). (d) Each welding procedure, welder, and fabricator must be approved....

  4. 7 CFR 1493.400 - General statement.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 10 2011-01-01 2011-01-01 false General statement. 1493.400 Section 1493.400 Agriculture Regulations of the Department of Agriculture (Continued) COMMODITY CREDIT CORPORATION, DEPARTMENT OF AGRICULTURE LOANS, PURCHASES, AND OTHER OPERATIONS CCC EXPORT CREDIT GUARANTEE PROGRAMS CCC Supplier Credit Guarantee Program Operations...

  5. 49 CFR 179.400-3 - Type.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 2 2010-10-01 2010-10-01 false Type. 179.400-3 Section 179.400-3 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION HAZARDOUS MATERIALS REGULATIONS SPECIFICATIONS FOR TANK CARS Specification for Cryogenic Liquid Tank Car Tanks...

  6. 7 CFR 400.409 - Unauthorized disclosure.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Unauthorized disclosure. 400.409 Section 400.409... Unauthorized disclosure. Anyone having access to the records identifying a participant's SSN or EIN will abide... section 6109(f), Internal Revenue Code of 1986 (26 U.S.C. 6109(f) and the Privacy Act of 1974 (5...

  7. 7 CFR 1710.400 - Initial contact.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 11 2014-01-01 2014-01-01 false Initial contact. 1710.400 Section 1710.400 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE GENERAL AND PRE-LOAN POLICIES AND PROCEDURES COMMON TO ELECTRIC LOANS AND GUARANTEES Application Requirements and Procedures for Loans...

  8. 15 CFR 400.15 - Production equipment.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Production equipment. 400.15 Section 400.15 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Ability...

  9. 49 CFR 179.400-5 - Materials.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 3 2012-10-01 2012-10-01 false Materials. 179.400-5 Section 179.400-5 Transportation Other Regulations Relating to Transportation (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) SPECIFICATIONS FOR TANK CARS Specification for Cryogenic Liquid Tank Car Tanks and...

  10. 46 CFR 119.400 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Applicability. 119.400 Section 119.400 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS CARRYING MORE THAN 150 PASSENGERS OR WITH OVERNIGHT ACCOMMODATIONS FOR MORE THAN 49 PASSENGERS MACHINERY INSTALLATION...

  11. 21 CFR 173.400 - Dimethyldialkylammonium chloride.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Dimethyldialkylammonium chloride. 173.400 Section 173.400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) SECONDARY DIRECT FOOD ADDITIVES PERMITTED IN FOOD FOR HUMAN CONSUMPTION Specific Usage Additives §...

  12. 21 CFR 173.400 - Dimethyldialkylammonium chloride.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Dimethyldialkylammonium chloride. 173.400 Section 173.400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) SECONDARY DIRECT FOOD ADDITIVES PERMITTED IN FOOD FOR HUMAN CONSUMPTION Specific Usage Additives §...

  13. 21 CFR 173.400 - Dimethyldialkylammonium chloride.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Dimethyldialkylammonium chloride. 173.400 Section 173.400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) SECONDARY DIRECT FOOD ADDITIVES PERMITTED IN FOOD FOR HUMAN CONSUMPTION Specific Usage Additives §...

  14. 7 CFR 400.115 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Purpose. 400.115 Section 400.115 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Debt Management-Regulations for the 1986 and Succeeding...

  15. 45 CFR 400.10 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false 400.10 Section 400.10 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Grants to States for...

  16. 45 CFR 400.6 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false 400.6 Section 400.6 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Grants to States for...

  17. 45 CFR 400.25 - Residency requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 2 2010-10-01 2010-10-01 false Residency requirements. 400.25 Section 400.25 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM...

  18. 21 CFR 1271.400 - Inspections.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Inspections. 1271.400 Section 1271.400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS, TISSUES,...

  19. 21 CFR 1271.400 - Inspections.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Inspections. 1271.400 Section 1271.400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS, TISSUES,...

  20. 21 CFR 1271.400 - Inspections.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Inspections. 1271.400 Section 1271.400 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) REGULATIONS UNDER CERTAIN OTHER ACTS ADMINISTERED BY THE FOOD AND DRUG ADMINISTRATION HUMAN CELLS, TISSUES,...

  1. 45 CFR 400.105 - Mandatory services.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... under the State's Medicaid program, as delineated in 42 CFR Part 440. ... 45 Public Welfare 2 2013-10-01 2012-10-01 true Mandatory services. 400.105 Section 400.105 Public... AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee...

  2. 45 CFR 400.23 - Hearings.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false Hearings. 400.23 Section 400.23 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration §...

  3. 45 CFR 400.25 - Residency requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false Residency requirements. 400.25 Section 400.25 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM...

  4. 45 CFR 400.23 - Hearings.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true Hearings. 400.23 Section 400.23 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM General Administration §...

  5. 45 CFR 400.6 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true 400.6 Section 400.6 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Grants to States for...

  6. 45 CFR 400.10 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false 400.10 Section 400.10 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Grants to States for...

  7. 45 CFR 400.25 - Residency requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true Residency requirements. 400.25 Section 400.25 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM...

  8. 45 CFR 400.6 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 2 2012-10-01 2012-10-01 false 400.6 Section 400.6 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Grants to States for...

  9. 45 CFR 400.10 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 2 2013-10-01 2012-10-01 true 400.10 Section 400.10 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Grants to States for...

  10. 7 CFR 735.400 - Administration.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 7 2011-01-01 2011-01-01 false Administration. 735.400 Section 735.400 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE... Administration. This subpart sets forth the regulations under which DACO may authorize one or more...

  11. 7 CFR 735.400 - Administration.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 7 2012-01-01 2012-01-01 false Administration. 735.400 Section 735.400 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE... Administration. This subpart sets forth the regulations under which DACO may authorize one or more...

  12. 7 CFR 735.400 - Administration.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Administration. 735.400 Section 735.400 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE... Administration. This subpart sets forth the regulations under which DACO may authorize one or more...

  13. 15 CFR 400.1 - Scope.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... regulations of the United States Customs Service (19 CFR part 146) governs zone operations, including the... merchandise from zones. (c) To the extent “activated” under Customs procedures in 19 CFR part 146, and only... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Scope. 400.1 Section 400.1...

  14. 7 CFR 400.652 - Insurance availability.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false Insurance availability. 400.652 Section 400.652 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Federal Crop Insurance Reform,...

  15. 7 CFR 400.98 - Reconsideration process.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... CFR part 11 before bringing suit against FCIC in a United States district court. However, regardless... 7 Agriculture 6 2012-01-01 2012-01-01 false Reconsideration process. 400.98 Section 400.98... process. (a) This reconsideration process only applies to determinations of good farming practices...

  16. 7 CFR 400.98 - Reconsideration process.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... CFR part 11 before bringing suit against FCIC in a United States district court. However, regardless... 7 Agriculture 6 2011-01-01 2011-01-01 false Reconsideration process. 400.98 Section 400.98... process. (a) This reconsideration process only applies to determinations of good farming practices...

  17. 45 CFR 1168.400 - Penalties.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 3 2012-10-01 2012-10-01 false Penalties. 1168.400 Section 1168.400 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE HUMANITIES NEW RESTRICTIONS ON LOBBYING Penalties and...

  18. 45 CFR 1168.400 - Penalties.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 3 2013-10-01 2013-10-01 false Penalties. 1168.400 Section 1168.400 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE HUMANITIES NEW RESTRICTIONS ON LOBBYING Penalties and...

  19. 45 CFR 1168.400 - Penalties.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 3 2014-10-01 2014-10-01 false Penalties. 1168.400 Section 1168.400 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE HUMANITIES NEW RESTRICTIONS ON LOBBYING Penalties and...

  20. 45 CFR 1168.400 - Penalties.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Penalties. 1168.400 Section 1168.400 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE HUMANITIES NEW RESTRICTIONS ON LOBBYING Penalties and...

  1. 45 CFR 1168.400 - Penalties.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 3 2011-10-01 2011-10-01 false Penalties. 1168.400 Section 1168.400 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE ARTS AND THE HUMANITIES NATIONAL ENDOWMENT FOR THE HUMANITIES NEW RESTRICTIONS ON LOBBYING Penalties and...

  2. 7 CFR 400.652 - Insurance availability.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Insurance availability. 400.652 Section 400.652 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS Federal Crop Insurance Reform,...

  3. 15 CFR 400.54 - Information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Information. 400.54 Section 400.54 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Records, Reports, Notice, Hearings and Information §...

  4. 15 CFR 400.54 - Information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Information. 400.54 Section 400.54 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Records, Reports, Notice, Hearings and Information §...

  5. 40 CFR 63.400 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 9 2010-07-01 2010-07-01 false Applicability. 63.400 Section 63.400 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) NATIONAL EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS FOR SOURCE CATEGORIES National Emission Standards for Hazardous Air Pollutants for...

  6. 41 CFR 101-39.400 - General.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 101-39.400 Public Contracts and Property Management Federal Property Management Regulations System... MANAGEMENT SYSTEMS 39.4-Accidents and Claims § 101-39.400 General. Officials, employees, and contractors responsible for the operation of General Services Administration (GSA) Interagency Fleet Management...

  7. 14 CFR 1252.400 - Compliance reviews.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 5 2011-01-01 2010-01-01 true Compliance reviews. 1252.400 Section 1252... OF AGE IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Investigation, Conciliation, and Enforcement Procedures § 1252.400 Compliance reviews. (a) NASA may conduct compliance reviews...

  8. 14 CFR 1252.400 - Compliance reviews.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 5 2013-01-01 2013-01-01 false Compliance reviews. 1252.400 Section 1252... OF AGE IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Investigation, Conciliation, and Enforcement Procedures § 1252.400 Compliance reviews. (a) NASA may conduct compliance reviews...

  9. 14 CFR 1252.400 - Compliance reviews.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 5 2012-01-01 2012-01-01 false Compliance reviews. 1252.400 Section 1252... OF AGE IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Investigation, Conciliation, and Enforcement Procedures § 1252.400 Compliance reviews. (a) NASA may conduct compliance reviews...

  10. 44 CFR 206.400 - General.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ....400 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY DISASTER ASSISTANCE FEDERAL DISASTER ASSISTANCE Minimum Standards § 206.400 General. (a) As a... regards to Executive Order 11988, Floodplain Management, Executive Order 12699, Seismic Safety of...

  11. 44 CFR 206.400 - General.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ....400 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY DISASTER ASSISTANCE FEDERAL DISASTER ASSISTANCE Minimum Standards § 206.400 General. (a) As a... regards to Executive Order 11988, Floodplain Management, Executive Order 12699, Seismic Safety of...

  12. 44 CFR 206.400 - General.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....400 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY DISASTER ASSISTANCE FEDERAL DISASTER ASSISTANCE Minimum Standards § 206.400 General. (a) As a... regards to Executive Order 11988, Floodplain Management, Executive Order 12699, Seismic Safety of...

  13. 31 CFR 1029.400 - General.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 31 Money and Finance:Treasury 3 2012-07-01 2012-07-01 false General. 1029.400 Section 1029.400 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued) FINANCIAL CRIMES ENFORCEMENT NETWORK, DEPARTMENT OF THE TREASURY RULES FOR LOAN OR FINANCE COMPANIES Records Required To...

  14. 7 CFR 65.400 - Labeling.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Labeling. 65.400 Section 65.400 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) REGULATIONS AND STANDARDS UNDER THE AGRICULTURAL MARKETING ACT OF 1946 AND THE...

  15. 7 CFR 65.400 - Labeling.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Labeling. 65.400 Section 65.400 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) REGULATIONS AND STANDARDS UNDER THE AGRICULTURAL MARKETING ACT OF 1946 AND THE...

  16. 7 CFR 65.400 - Labeling.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Labeling. 65.400 Section 65.400 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) REGULATIONS AND STANDARDS UNDER THE AGRICULTURAL MARKETING ACT OF 1946 AND THE...

  17. 7 CFR 65.400 - Labeling.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Labeling. 65.400 Section 65.400 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) REGULATIONS AND STANDARDS UNDER THE AGRICULTURAL MARKETING ACT OF 1946 AND THE...

  18. 15 CFR 400.45 - Retail trade.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 2 2011-01-01 2011-01-01 false Retail trade. 400.45 Section 400.45 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Zone Operations...

  19. 15 CFR 400.47 - Retail trade.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 2 2014-01-01 2014-01-01 false Retail trade. 400.47 Section 400.47 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Operation of Zones...

  20. 15 CFR 400.47 - Retail trade.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 15 Commerce and Foreign Trade 2 2013-01-01 2013-01-01 false Retail trade. 400.47 Section 400.47 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Operation of Zones...

  1. 15 CFR 400.45 - Retail trade.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 15 Commerce and Foreign Trade 2 2012-01-01 2012-01-01 false Retail trade. 400.45 Section 400.45 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Zone Operations...

  2. 15 CFR 400.45 - Retail trade.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 2 2010-01-01 2010-01-01 false Retail trade. 400.45 Section 400.45 Commerce and Foreign Trade Regulations Relating to Commerce and Foreign Trade (Continued) FOREIGN-TRADE ZONES BOARD, DEPARTMENT OF COMMERCE REGULATIONS OF THE FOREIGN-TRADE ZONES BOARD Zone Operations...

  3. 38 CFR 41.400 - Responsibilities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2010-07-01 2010-07-01 false Responsibilities. 41.400... § 41.400 Responsibilities. (a) Cognizant agency for audit responsibilities. Recipients expending more... audit work and reporting responsibilities among auditors to achieve the most cost-effective audit....

  4. 38 CFR 41.400 - Responsibilities.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 2 2012-07-01 2012-07-01 false Responsibilities. 41.400... § 41.400 Responsibilities. (a) Cognizant agency for audit responsibilities. Recipients expending more... audit work and reporting responsibilities among auditors to achieve the most cost-effective audit....

  5. 7 CFR 400.404 - Policyholder responsibilities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false Policyholder responsibilities. 400.404 Section 400.404 Agriculture Regulations of the Department of Agriculture (Continued) FEDERAL CROP INSURANCE CORPORATION, DEPARTMENT OF AGRICULTURE GENERAL ADMINISTRATIVE REGULATIONS General Administrative Regulations; Collection and Storage of Social...

  6. 45 CFR 400.301 - Withdrawal from the refugee program.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... exception of the following provisions: 45 CFR 400.5(d), 400.7, 400.51(b)(2)(i), 400.58(c), 400.94(a), 400.94... 45 Public Welfare 2 2014-10-01 2012-10-01 true Withdrawal from the refugee program. 400.301 Section 400.301 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE...

  7. 45 CFR 400.301 - Withdrawal from the refugee program.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... exception of the following provisions: 45 CFR 400.5(d), 400.7, 400.51(b)(2)(i), 400.58(c), 400.94(a), 400.94... 45 Public Welfare 2 2011-10-01 2011-10-01 false Withdrawal from the refugee program. 400.301 Section 400.301 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE...

  8. 45 CFR 400.301 - Withdrawal from the refugee program.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... exception of the following provisions: 45 CFR 400.5(d), 400.7, 400.51(b)(2)(i), 400.58(c), 400.94(a), 400.94... 45 Public Welfare 2 2010-10-01 2010-10-01 false Withdrawal from the refugee program. 400.301 Section 400.301 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE...

  9. 45 CFR 400.301 - Withdrawal from the refugee program.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... exception of the following provisions: 45 CFR 400.5(d), 400.7, 400.51(b)(2)(i), 400.58(c), 400.94(a), 400.94... 45 Public Welfare 2 2012-10-01 2012-10-01 false Withdrawal from the refugee program. 400.301 Section 400.301 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE...

  10. 45 CFR 400.301 - Withdrawal from the refugee program.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... exception of the following provisions: 45 CFR 400.5(d), 400.7, 400.51(b)(2)(i), 400.58(c), 400.94(a), 400.94... 45 Public Welfare 2 2013-10-01 2012-10-01 true Withdrawal from the refugee program. 400.301 Section 400.301 Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE...

  11. 38 CFR 3.400 - General.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... Editorial Note: For Federal Register citations affecting § 3.400, see the List of CFR Sections Affected... Forces Service Pilots (WASP), or on service in a similarly situated group (Pub. L. 95-202). (1)...

  12. 38 CFR 3.400 - General.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... Editorial Note: For Federal Register citations affecting § 3.400, see the List of CFR Sections Affected... Forces Service Pilots (WASP), or on service in a similarly situated group (Pub. L. 95-202). (1)...

  13. 38 CFR 3.400 - General.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... Editorial Note: For Federal Register citations affecting § 3.400, see the List of CFR Sections Affected... Forces Service Pilots (WASP), or on service in a similarly situated group (Pub. L. 95-202). (1)...

  14. 9 CFR 93.400 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... education. Editorial Note: For Federal Register citations affecting § 93.400, see the List of CFR Sections... deer, elk, moose, caribou, reindeer, and related species. Communicable disease. Any...

  15. 9 CFR 93.400 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... education. Editorial Note: For Federal Register citations affecting § 93.400, see the List of CFR Sections... deer, elk, moose, caribou, reindeer, and related species. Communicable disease. Any...

  16. 9 CFR 93.400 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... education. Editorial Note: For Federal Register citations affecting § 93.400, see the List of CFR Sections... deer, elk, moose, caribou, reindeer, and related species. Communicable disease. Any...

  17. 9 CFR 93.400 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... education. Editorial Note: For Federal Register citations affecting § 93.400, see the List of CFR Sections... deer, elk, moose, caribou, reindeer, and related species. Communicable disease. Any...

  18. 9 CFR 93.400 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... education. Editorial Note: For Federal Register citations affecting § 93.400, see the List of CFR Sections... deer, elk, moose, caribou, reindeer, and related species. Communicable disease. Any...

  19. 7 CFR 400.677 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... charges (7 CFR 400.116). A delinquent debt does not include debts discharged in bankruptcy and other debts... (cannabis sativa), opium poppies (papaver somniferum), and other drug-producing plants, the planting...

  20. 7 CFR 400.677 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... charges (7 CFR 400.116). A delinquent debt does not include debts discharged in bankruptcy and other debts... (cannabis sativa), opium poppies (papaver somniferum), and other drug-producing plants, the planting...

  1. 7 CFR 400.677 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... charges (7 CFR 400.116). A delinquent debt does not include debts discharged in bankruptcy and other debts... (cannabis sativa), opium poppies (papaver somniferum), and other drug-producing plants, the planting...

  2. 7 CFR 400.677 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... charges (7 CFR 400.116). A delinquent debt does not include debts discharged in bankruptcy and other debts... (cannabis sativa), opium poppies (papaver somniferum), and other drug-producing plants, the planting...

  3. 7 CFR 400.677 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... charges (7 CFR 400.116). A delinquent debt does not include debts discharged in bankruptcy and other debts... (cannabis sativa), opium poppies (papaver somniferum), and other drug-producing plants, the planting...

  4. 7 CFR 360.400 - Treatments.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., DEPARTMENT OF AGRICULTURE NOXIOUS WEED REGULATIONS § 360.400 Treatments. (a) Seeds of Guizotia abyssinica (niger seed) are commonly contaminated with noxious weed seeds listed in § 360.200, including (but...

  5. 7 CFR 360.400 - Treatments.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., DEPARTMENT OF AGRICULTURE NOXIOUS WEED REGULATIONS § 360.400 Treatments. (a) Seeds of Guizotia abyssinica (niger seed) are commonly contaminated with noxious weed seeds listed in § 360.200, including (but...

  6. 7 CFR 360.400 - Treatments.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., DEPARTMENT OF AGRICULTURE NOXIOUS WEED REGULATIONS § 360.400 Treatments. (a) Seeds of Guizotia abyssinica (niger seed) are commonly contaminated with noxious weed seeds listed in § 360.200, including (but...

  7. 7 CFR 360.400 - Treatments.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., DEPARTMENT OF AGRICULTURE NOXIOUS WEED REGULATIONS § 360.400 Treatments. (a) Seeds of Guizotia abyssinica (niger seed) are commonly contaminated with noxious weed seeds listed in § 360.200, including (but...

  8. 40 CFR 63.400 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Hazardous Air Pollutants for Industrial Process Cooling Towers § 63.400 Applicability. (a) The provisions of this subpart apply to all new and existing industrial process cooling towers that are operated...

  9. 48 CFR 9904.400 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Federal Acquisition Regulations System COST ACCOUNTING STANDARDS BOARD, OFFICE OF FEDERAL PROCUREMENT POLICY, OFFICE OF MANAGEMENT AND BUDGET PROCUREMENT PRACTICES AND COST ACCOUNTING STANDARDS COST ACCOUNTING STANDARDS 9904.400...

  10. 48 CFR 9904.400 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Federal Acquisition Regulations System COST ACCOUNTING STANDARDS BOARD, OFFICE OF FEDERAL PROCUREMENT POLICY, OFFICE OF MANAGEMENT AND BUDGET PROCUREMENT PRACTICES AND COST ACCOUNTING STANDARDS COST ACCOUNTING STANDARDS 9904.400...

  11. 48 CFR 9904.400 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Federal Acquisition Regulations System COST ACCOUNTING STANDARDS BOARD, OFFICE OF FEDERAL PROCUREMENT POLICY, OFFICE OF MANAGEMENT AND BUDGET PROCUREMENT PRACTICES AND COST ACCOUNTING STANDARDS COST ACCOUNTING STANDARDS 9904.400...

  12. 48 CFR 9904.400 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Federal Acquisition Regulations System COST ACCOUNTING STANDARDS BOARD, OFFICE OF FEDERAL PROCUREMENT POLICY, OFFICE OF MANAGEMENT AND BUDGET PROCUREMENT PRACTICES AND COST ACCOUNTING STANDARDS COST ACCOUNTING STANDARDS 9904.400...

  13. 40 CFR 63.400 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Hazardous Air Pollutants for Industrial Process Cooling Towers § 63.400 Applicability. (a) The provisions of this subpart apply to all new and existing industrial process cooling towers that are operated...

  14. 40 CFR 63.400 - Applicability.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Hazardous Air Pollutants for Industrial Process Cooling Towers § 63.400 Applicability. (a) The provisions of this subpart apply to all new and existing industrial process cooling towers that are operated...

  15. 40 CFR 63.400 - Applicability.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Hazardous Air Pollutants for Industrial Process Cooling Towers § 63.400 Applicability. (a) The provisions of this subpart apply to all new and existing industrial process cooling towers that are operated...

  16. 46 CFR 182.400 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) MACHINERY INSTALLATION Specific Machinery Requirements § 182.400 Applicability. (a) This subpart applies to all propulsion and auxiliary machinery installations of the internal combustion piston type. (b... fuels and machinery....

  17. 34 CFR 300.709 - Public participation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Public participation. 300.709 Section 300.709 Education... Interior § 300.709 Public participation. In fulfilling the requirements of § 300.708 the Secretary of the Interior must provide for public participation consistent with § 300.165. (Authority: 20 U.S.C. 1411(h))...

  18. Heterogeneous expression of zinc-finger E-box-binding homeobox 1 plays a pivotal role in metastasis via regulation of miR-200c in epithelial-mesenchymal transition.

    PubMed

    Muto, Yuta; Suzuki, Koichi; Kato, Takaharu; Tsujinaka, Shingo; Ichida, Kosuke; Takayama, Yuji; Fukui, Taro; Kakizawa, Nao; Watanabe, Fumiaki; Saito, Masaaki; Futsuhara, Kazushige; Noda, Hiroshi; Miyakura, Yasuyuki; Konishi, Fumio; Rikiyama, Toshiki

    2016-09-01

    Although epithelial-mesenchymal transition (EMT) has been implicated as the pivotal event in metastasis, there is insufficient evidence related to EMT in clinical settings. Intratumor heterogeneity may lead to underestimation of gene expression representing EMT. In the present study, we investigated the expression of EMT-associated genes and microRNAs in primary colorectal cancer while considering intratumor heterogeneity. One-hundred and thirty-three multiple spatially separated samples were obtained from 8 patients with metastatic colorectal cancers and 8 with non-metastatic colorectal cancers, from the tumor center (TC), invasive front (IF) and metastasis. Differences in gene and microRNA expression were investigated by microarray and quantitative reverse-transcription PCR. Gene expression microarray analysis detected 7920 sites showing differing levels of gene expression among the TC, IF and metastasis. Expression of the EMT-associated gene zinc-finger E-box-binding homeobox 1 (ZEB1) significantly increased in the IF (p<0.01). To exclude individual differences, the expression ratio between TC and IF in each tumor was applied to analysis. This approach enabled recognition of the activation of the VEGF and Wnt signaling pathways, which were involved in metastasis via promotion of EMT. While no activation of these pathways was seen at the TC, regardless of whether tumors were metastatic or non-metastatic, they were preferentially activated at the IF in metastatic tumors, where high ZEB1 expression was seen in connection with decreased miR-200c expression. Multiple sampling in a tumor revealed that heterogeneous ZEB1 expression induced by EMT-associated signaling pathways played a pivotal role in metastasis via regulation of miR-200c. PMID:27315529

  19. 50 CFR 300.39 - Exceptions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Pacific Tuna Fisheries § 300.39 Exceptions. (a) The prohibitions of § 300.38 and the licensing requirements of § 300.32 do not apply to fishing for albacore tuna by vessels using the trolling method or...

  20. 50 CFR 300.39 - Exceptions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Pacific Tuna Fisheries § 300.39 Exceptions. (a) The prohibitions of § 300.38 and the licensing requirements of § 300.32 do not apply to fishing for albacore tuna by vessels using the trolling method or...

  1. The HWP-300 wind turbine

    NASA Astrophysics Data System (ADS)

    Jamieson, P.; McLeish, D.

    1983-12-01

    A 300 kW, 22 m diameter wind turbine has been designed and erected (July 1983) on the site of the North of Scotland Hydro-Electric Board at Burgar Hill in Orkney. The machine and its design concepts are described.

  2. Chemistry 200, 300 Interim Guide.

    ERIC Educational Resources Information Center

    Manitoba Dept. of Education, Winnipeg.

    This guide, developed for the chemistry 200, 300 program in Manitoba, is designed to articulate with previous science courses, provide concepts, processes, and skills which will enable students to continue in chemistry-related areas, and relate chemistry to practical applications in everyday life. It includes a program overview (with program goals…

  3. Physics 200, 300 Interim Guide.

    ERIC Educational Resources Information Center

    Manitoba Dept. of Education, Winnipeg.

    This guide, developed for the physics 200, 300 program in Manitoba, is designed to articulate with previous science courses; provide concepts, processes, and skills which will enable students to continue in physics-related areas; and relate physics to practical applications in everyday life. It includes a program overview (with program goals and…

  4. The Effect of Composition on the Surface Finish of PS400: A New High Temperature Solid Lubricant Coating

    NASA Technical Reports Server (NTRS)

    DellaCorte, Christopher; Stanford, malcolm K.; Thomas, Fransua; Edmonds, Brian J.

    2010-01-01

    A new composite, multi-constituent, solid lubricant coating, NASA PS400, developed for high temperature tribological applications, exhibits a smoother surface finish after grinding and polishing than its predecessors PS200 and PS300. In this paper, the baseline composition of PS400 is modified to investigate each individual constituent s role on the achievable surface finish through a series of coating deposition, grinding, and polishing experiments. Furthermore, to explore the limits of compositional tailoring for improved tribological performance, several PS400 coatings were doped with additional solid lubricants (graphite, MoS2 and BN) and tribologically tested. The test results clearly showed that, compared to PS300 coatings, PS400 achieves a smoother surface finish via a reduced lubricant content. Coatings prepared with higher than the baseline level (10 wt%) of lubricants exhibited higher final surface roughness than the earlier generation PS300 coatings. Reducing or eliminating the one or both lubricants (fluorides or silver) did not further improve the surface finish suggesting that the current composition of PS400 is near optimal with respect to surface finish. Lastly, attempts to improve the poor initial room temperature tribological behavior of PS400 via the addition of traditional solid lubricants were unsuccessful. Based upon this work and earlier results it is expected that future research will concentrate on developing methods to produce a lubricious glaze on the rubbing surface during break in to ensure that low friction and wear are rapidly achieved.

  5. 75 FR 3147 - Airworthiness Directives; The Boeing Company Model 747-100B SUD, -200B, -300, -400, and -400D...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-20

    ... 26, 2003, as of August 30, 2005 (70 FR 43020, July 26, 2005). ADDRESSES: For service information..., amendment 39-14197 (70 FR 43020, July 26, 2005). The existing AD applies to certain Model 747-100B SUD... 14, 2009 (74 FR 33928). That NPRM proposed to require repetitive inspections for cracking in...

  6. Nuclear criticality safety: 300 Area

    SciTech Connect

    Not Available

    1991-07-31

    This Standard applies to the receipt, processing, storage, and shipment of fissionable material in the 300 Area and in any other facility under the control of the Reactor Materials Project Management Team (PMT). The objective is to establish practices and process conditions for the storage and handling of fissionable material that prevent the accidental assembly of a critical mass and that comply with DOE Orders as well as accepted industry practice.

  7. 50 CFR 300.117 - Prohibitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Prohibitions. 300.117 Section 300.117 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Antarctic Marine Living Resources § 300.117 Prohibitions. In addition to the prohibitions in § 300.4, it is unlawful for any person to: (a) Reduce...

  8. 34 CFR 300.117 - Nonacademic settings.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Nonacademic settings. 300.117 Section 300.117 Education... DISABILITIES State Eligibility Least Restrictive Environment (lre) § 300.117 Nonacademic settings. In providing... meals, recess periods, and the services and activities set forth in § 300.107, each public agency...

  9. 11 CFR 300.2 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... connection with a Federal election as that term is used in 11 CFR 300.11, 300.37, 300.50, and 300.51 includes... a public communication (see 11 CFR 100.26). (c) Directly or indirectly establish, finance, maintain... its allocated share of proceeds jointly raised pursuant to 11 CFR 102.17, and otherwise...

  10. 11 CFR 300.2 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... connection with a Federal election as that term is used in 11 CFR 300.11, 300.37, 300.50, and 300.51 includes... a public communication (see 11 CFR 100.26). (c) Directly or indirectly establish, finance, maintain... its allocated share of proceeds jointly raised pursuant to 11 CFR 102.17, and otherwise...

  11. 34 CFR 300.327 - Educational placements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Educational placements. 300.327 Section 300.327... Educational Placements Development of Iep § 300.327 Educational placements. Consistent with § 300.501(c), each... makes decisions on the educational placement of their child. (Authority: 20 U.S.C. 1414(e))...

  12. 34 CFR 300.613 - Access rights.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Access rights. 300.613 Section 300.613 Education... § 300.613 Access rights. (a) Each participating agency must permit parents to inspect and review any... pursuant to § 300.510, and in no case more than 45 days after the request has been made. (b) The right...

  13. 34 CFR 300.512 - Hearing rights.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Hearing rights. 300.512 Section 300.512 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.512 Hearing rights. (a....534, or an appeal conducted pursuant to § 300.514, has the right to— (1) Be accompanied and advised...

  14. 50 CFR 300.104 - Scientific research.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 11 2013-10-01 2013-10-01 false Scientific research. 300.104 Section 300.104 Wildlife and Fisheries INTERNATIONAL FISHING AND RELATED ACTIVITIES INTERNATIONAL FISHERIES REGULATIONS Antarctic Marine Living Resources § 300.104 Scientific research. (a) The management measures issued pursuant to the procedures at § 300.111...

  15. 34 CFR 300.623 - Safeguards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... instruction regarding the State's policies and procedures under § 300.123 and 34 CFR part 99. (d) Each... 34 Education 2 2010-07-01 2010-07-01 false Safeguards. 300.623 Section 300.623 Education... § 300.623 Safeguards. (a) Each participating agency must protect the confidentiality of...

  16. 34 CFR 300.623 - Safeguards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... instruction regarding the State's policies and procedures under § 300.123 and 34 CFR part 99. (d) Each... 34 Education 2 2011-07-01 2010-07-01 true Safeguards. 300.623 Section 300.623 Education... § 300.623 Safeguards. (a) Each participating agency must protect the confidentiality of...

  17. 34 CFR 300.121 - Procedural safeguards.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Procedural safeguards. 300.121 Section 300.121... safeguards. (a) General. The State must have procedural safeguards in effect to ensure that each public agency in the State meets the requirements of §§ 300.500 through 300.536. (b) Procedural...

  18. 34 CFR 300.516 - Civil action.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 2 2012-07-01 2012-07-01 false Civil action. 300.516 Section 300.516 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.516 Civil action. (a... aggrieved by the findings and decision under § 300.514(b), has the right to bring a civil action...

  19. 34 CFR 300.516 - Civil action.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 2 2014-07-01 2013-07-01 true Civil action. 300.516 Section 300.516 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.516 Civil action. (a... aggrieved by the findings and decision under § 300.514(b), has the right to bring a civil action...

  20. 34 CFR 300.516 - Civil action.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 2 2013-07-01 2013-07-01 false Civil action. 300.516 Section 300.516 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.516 Civil action. (a... aggrieved by the findings and decision under § 300.514(b), has the right to bring a civil action...

  1. 34 CFR 300.516 - Civil action.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Civil action. 300.516 Section 300.516 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.516 Civil action. (a... aggrieved by the findings and decision under § 300.514(b), has the right to bring a civil action...

  2. 50 CFR 300.125 - Vessel identification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 9 2011-10-01 2011-10-01 false Vessel identification. 300.125 Section 300... REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.125 Vessel identification. (a) Official number. A vessel with a permit issued pursuant to § 300.123, when in treaty waters,...

  3. 50 CFR 300.125 - Vessel identification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 7 2010-10-01 2010-10-01 false Vessel identification. 300.125 Section 300... REGULATIONS Vessels of the United States Fishing in Colombian Treaty Waters § 300.125 Vessel identification. (a) Official number. A vessel with a permit issued pursuant to § 300.123, when in treaty waters,...

  4. 34 CFR 300.516 - Civil action.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Civil action. 300.516 Section 300.516 Education... DISABILITIES Procedural Safeguards Due Process Procedures for Parents and Children § 300.516 Civil action. (a... aggrieved by the findings and decision under § 300.514(b), has the right to bring a civil action...

  5. 33 CFR 103.400 - General.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... SECURITY: AREA MARITIME SECURITY Area Maritime Security (AMS) Assessment § 103.400 General. (a) The Area Maritime Security (AMS) Committee will ensure that a risk based AMS Assessment, is completed and meets the... Coast Guard Port Security Assessment team, or by another third party approved by the AMS Committee....

  6. 24 CFR 597.400 - Reporting.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... COMMUNITY FACILITIES URBAN EMPOWERMENT ZONES AND ENTERPRISE COMMUNITIES: ROUND ONE DESIGNATIONS Post-Designation Requirements § 597.400 Reporting. HUD will require periodic reports for the Empowerment Zones and... these reports, such other information relating to designated Empowerment Zones and...

  7. 31 CFR 1028.400 - General.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... ENFORCEMENT NETWORK, DEPARTMENT OF THE TREASURY RULES FOR OPERATORS OF CREDIT CARD SYSTEMS Records Required To Be Maintained By Operators of Credit Card Systems § 1028.400 General. Operators of credit card.... Operators of credit card systems should also refer to Subpart D of Part 1010 of this Chapter...

  8. 31 CFR 1028.400 - General.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... ENFORCEMENT NETWORK, DEPARTMENT OF THE TREASURY RULES FOR OPERATORS OF CREDIT CARD SYSTEMS Records Required To Be Maintained By Operators of Credit Card Systems § 1028.400 General. Operators of credit card.... Operators of credit card systems should also refer to Subpart D of Part 1010 of this Chapter...

  9. 31 CFR 1028.400 - General.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... ENFORCEMENT NETWORK, DEPARTMENT OF THE TREASURY RULES FOR OPERATORS OF CREDIT CARD SYSTEMS Records Required To Be Maintained By Operators of Credit Card Systems § 1028.400 General. Operators of credit card.... Operators of credit card systems should also refer to Subpart D of Part 1010 of this Chapter...

  10. 31 CFR 1028.400 - General.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... ENFORCEMENT NETWORK, DEPARTMENT OF THE TREASURY RULES FOR OPERATORS OF CREDIT CARD SYSTEMS Records Required To Be Maintained By Operators of Credit Card Systems § 1028.400 General. Operators of credit card.... Operators of credit card systems should also refer to Subpart D of Part 1010 of this Chapter...

  11. 45 CFR 400.154 - Employability services.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Public Welfare Regulations Relating to Public Welfare OFFICE OF REFUGEE RESETTLEMENT, ADMINISTRATION FOR CHILDREN AND FAMILIES, DEPARTMENT OF HEALTH AND HUMAN SERVICES REFUGEE RESETTLEMENT PROGRAM Refugee Social Services Scope of Refugee Social Services § 400.154 Employability services. A State may provide...

  12. 13 CFR 400.202 - Loan amount.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ....202 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.202 Loan amount. (a) The aggregate amount of loan principal guaranteed under this Program to a single Qualified Steel Company may not exceed $ 250 million. (b) Of...

  13. 13 CFR 400.202 - Loan amount.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ....202 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.202 Loan amount. (a) The aggregate amount of loan principal guaranteed under this Program to a single Qualified Steel Company may not exceed $ 250 million. (b) Of...

  14. 13 CFR 400.202 - Loan amount.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ....202 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.202 Loan amount. (a) The aggregate amount of loan principal guaranteed under this Program to a single Qualified Steel Company may not exceed $ 250 million. (b) Of...

  15. 13 CFR 400.202 - Loan amount.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ....202 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.202 Loan amount. (a) The aggregate amount of loan principal guaranteed under this Program to a single Qualified Steel Company may not exceed $ 250 million. (b) Of...

  16. 13 CFR 400.202 - Loan amount.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ....202 Business Credit and Assistance EMERGENCY STEEL GUARANTEE LOAN BOARD EMERGENCY STEEL GUARANTEE LOAN PROGRAM Steel Guarantee Loans § 400.202 Loan amount. (a) The aggregate amount of loan principal guaranteed under this Program to a single Qualified Steel Company may not exceed $ 250 million. (b) Of...

  17. 7 CFR 25.400 - Reporting.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Requirements § 25.400 Reporting. (a) Periodic reports. Empowerment Zones, Enterprise Communities and Champion Communities shall submit to USDA periodic reports which identify the community, local government and State... information and of on-site reviews, USDA will prepare and issue periodic reports on the effectiveness of...

  18. 38 CFR 3.400 - General.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... Editorial Note: For Federal Register citations affecting § 3.400, see the List of CFR Sections Affected... under the Federal Employees' Compensation Act over the same period of time. (g) Correction of military..., change or modification of a military record, or of a discharge or dismissal, by a Board established...

  19. 38 CFR 3.400 - General.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... Editorial Note: For Federal Register citations affecting § 3.400, see the List of CFR Sections Affected... under the Federal Employees' Compensation Act over the same period of time. (g) Correction of military..., change or modification of a military record, or of a discharge or dismissal, by a Board established...

  20. 33 CFR 158.400 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... FACILITIES FOR OIL, NOXIOUS LIQUID SUBSTANCES, AND GARBAGE Criteria for Adequacy of Reception Facilities: Garbage § 158.400 Purpose. The purpose of this subpart is to supply the criteria for determining the adequacy of reception facilities for garbage at ports and terminals that receive ships and to comply...