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Sample records for 30s 31cl deduced

  1. Measurement of the longitudinal momentum distribution of {sup 30}S after one-proton removal from {sup 31}Cl

    SciTech Connect

    Fu, Y.; Fang, D. Q.; Ma, Y. G.; Cai, X. Z.; Tian, W. D.; Wang, H. W.; Guo, W.; Liu, G. H.; Ma, C. W.; Fan, R. R.; Fu, F.; Gao, H.; Gao, Q.; Guo, W. T.; Han, J. L.; Hu, Z. G.; Huang, T. H.; Jia, F.; Li, B.; Lei, X. G.

    2011-09-15

    The breakup reactions of {sup 31}Cl+{sup 12}C at 44A MeV are investigated experimentally. The longitudinal momentum distribution of {sup 30}S after one-proton removal from {sup 31}Cl has been obtained. By assuming core plus a valence proton structure, the momentum distribution is studied using the few-body Glauber model. The calculation shows that the width of momentum distribution for a valence proton with l=2 is consistent with the experimental data, which indicates a dominant d-wave component for the valence proton in {sup 31}Cl.

  2. A dye-sensitized visible light photocatalyst-Bi24O31Cl10

    PubMed Central

    Wang, Liang; Shang, Jun; Hao, Weichang; Jiang, Shiqi; Huang, Shiheng; Wang, Tianmin; Sun, Ziqi; Du, Yi; Dou, Shixue; Xie, Tengfeng; Wang, Dejun; Wang, Jiaou

    2014-01-01

    The p-block semiconductors are regarded as a new family of visible-light photocatalysts because of their dispersive and anisotropic band structures as well as high chemical stability. The bismuth oxide halides belong to this family and have band structures and dispersion relations that can be engineered by modulating the stoichiometry of the halogen elements. Herein, we have developed a new visible-light photocatalyst Bi24O31Cl10 by band engineering, which shows high dye-sensitized photocatalytic activity. Density functional theory calculations reveal that the p-block elements determine the nature of the dispersive electronic structures and narrow band gap in Bi24O31Cl10. Bi24O31Cl10 exhibits excellent visible-light photocatalytic activity towards the degradation of Rhodamine B, which is promoted by dye sensitization due to compatible energy levels and high electronic mobility. In addition, Bi24O31Cl10 is also a suitable photoanode material for dye-sensitized solar cells and shows power conversion efficiency of 1.5%. PMID:25488704

  3. 31Cl beta decay and the 30P31S reaction rate in nova nucleosynthesis

    NASA Astrophysics Data System (ADS)

    Bennett, Michael; Wrede, C.; Brown, B. A.; Liddick, S. N.; Pérez-Loureiro, D.; NSCL e12028 Collaboration

    2016-03-01

    The 30P31S reaction rate is critical for modeling the final isotopic abundances of ONe nova nucleosynthesis, identifying the origin of presolar nova grains, and calibrating proposed nova thermometers. Unfortunately, this rate is essentially experimentally unconstrained because the strengths of key 31S proton capture resonances are not known, due to uncertainties in their spins and parities. Using a 31Cl beam produced at the National Superconducting Cyclotron Laboratory, we have populated several 31S states for study via beta decay and devised a new decay scheme which includes updated beta feedings and gamma branchings as well as multiple states previously unobserved in 31Cl beta decay. Results of this study, including the unambiguous identification due to isospin mixing of a new l = 0 , Jπ = 3 /2+ 31S resonance directly in the middle of the Gamow Window, will be presented, and significance to the evaluation of the 30P31S reaction rate will be discussed. Work supported by U.S. Natl. Sci. Foundation (Grants No. PHY-1102511, PHY-1404442, PHY-1419765, and PHY-1431052); U.S. Dept. of Energy, Natl. Nucl. Security Administration (Award No. DE-NA0000979); Nat. Sci. and Eng. Research Council of Canada.

  4. Assembly of the 30S ribosomal subunit.

    PubMed

    Williamson, James R

    2005-11-01

    The assembly of ribosomes requires a significant fraction of the energy expenditure for rapidly growing bacteria. The ribosome is composed of three large RNA molecules and over 50 small proteins that must be rapidly and efficiently assembled into the molecular machine responsible for protein synthesis. For over 30 years, the 30S ribosome has been a key model system for understanding the process of ribosome biogenesis through in vitro assembly experiments. We have recently developed an isotope pulse-chase experiment using quantitative mass spectrometry that permits assembly kinetics to be measured in real time. Kinetic studies have revealed an assembly energy landscape that ensures efficient assembly by a flexible and robust pathway.

  5. Constructing Bi24O31Cl10/BiOCl heterojunction via a simple thermal annealing route for achieving enhanced photocatalytic activity and selectivity

    PubMed Central

    Liu, Xiaoyan; Su, Yiguo; Zhao, Qihang; Du, Chunfang; Liu, Zhiliang

    2016-01-01

    This work reports on the construction of a Bi24O31Cl10/BiOCl heterojunction via a simple thermal annealing method. The X-ray diffraction (XRD) results indicated that the phase transformation from BiOCl to Bi24O31Cl10 could be realized during the thermal annealing process. The high-resolution transmission electron microscopy (HRTEM) images, X-ray photoelectron spectroscopy (XPS) binding energy shifts, Raman spectra and Fouier transform infrared spectroscopy (FT-IR) spectra confirmed the formation of the Bi24O31Cl10/BiOCl heterojunction. The obtained Bi24O31Cl10/BiOCl photocatalyst showed excellent conversion efficiency and selectivity toward photocatalytic conversion of benzyl alcohol to benzaldehyde under visible light irradiation. The radical scavengers and electron spin resonance (ESR) results suggested that the photogenerated holes were the dominant reactive species responsible for the photocatalytic oxidation of benzyl alcohol and superoxide radicals were not involved in the photocatalytic process. The in-situ generation of Bi24O31Cl10/BiOCl heterojunction may own superior interfacial contact than the two-step synthesized heterojunctions, which promotes the transfer of photogenerated charge carriers and is favorable for excellent photocatalytic activities. PMID:27340032

  6. Constructing Bi24O31Cl10/BiOCl heterojunction via a simple thermal annealing route for achieving enhanced photocatalytic activity and selectivity

    NASA Astrophysics Data System (ADS)

    Liu, Xiaoyan; Su, Yiguo; Zhao, Qihang; Du, Chunfang; Liu, Zhiliang

    2016-06-01

    This work reports on the construction of a Bi24O31Cl10/BiOCl heterojunction via a simple thermal annealing method. The X-ray diffraction (XRD) results indicated that the phase transformation from BiOCl to Bi24O31Cl10 could be realized during the thermal annealing process. The high-resolution transmission electron microscopy (HRTEM) images, X-ray photoelectron spectroscopy (XPS) binding energy shifts, Raman spectra and Fouier transform infrared spectroscopy (FT-IR) spectra confirmed the formation of the Bi24O31Cl10/BiOCl heterojunction. The obtained Bi24O31Cl10/BiOCl photocatalyst showed excellent conversion efficiency and selectivity toward photocatalytic conversion of benzyl alcohol to benzaldehyde under visible light irradiation. The radical scavengers and electron spin resonance (ESR) results suggested that the photogenerated holes were the dominant reactive species responsible for the photocatalytic oxidation of benzyl alcohol and superoxide radicals were not involved in the photocatalytic process. The in-situ generation of Bi24O31Cl10/BiOCl heterojunction may own superior interfacial contact than the two-step synthesized heterojunctions, which promotes the transfer of photogenerated charge carriers and is favorable for excellent photocatalytic activities.

  7. Study of excited states of {sup 31}S through beta-decay of {sup 31}Cl for nucleosynthesis in ONe novae

    SciTech Connect

    Saastamoinen, A.; Jokinen, A.; Aeystoe, J.; Trache, L.; Hardy, J. C.; Iacob, V. E.; McCleskey, M.; Roeder, B.; Simmons, E.; Tabacaru, G.; Tribble, R. E.; Banu, A.; Bentley, M. A.; Davinson, T.; Woods, P. J.

    2011-11-30

    We have produced an intense and pure beam of {sup 31}Cl with the MARS Separator at the Texas A and M University and studied {beta}-decay of {sup 31}Cl by implanting the beam into a novel detector setup, capable of measuring {beta}-delayed protons and {gamma}-rays simultaneously. From our data, we have established decay scheme of {sup 31}Cl, found resonance energies with 1 keV precision, have measured its half-life with under 1% accuracy, found its Isobar Analog State decay and by using the IMME obtained an improved mass excess for its ground state. In this contribution, a description of the used method along with selected preliminary experimental results are given and their relevance for novae nucleosynthesis discussed.

  8. Beta-decay of 31Cl: an indirect probe of the 30P(p,γ)31S reaction. Present status and future perspectives

    NASA Astrophysics Data System (ADS)

    Saastamoinen, Antti; Kankainen, Anu; Trache, Livius

    2016-08-01

    β-decay of 31Cl can be used as a selective tool for studying astrophysically relevant states in 31S. In this article we review the present status of the decay data. The implications for the 30P(p,γ)31S reaction rate at novae temperatures, and future experimental ideas are discussed.

  9. Nuclear structure of 30S and its implications for nucleosynthesis in classical novae

    NASA Astrophysics Data System (ADS)

    Setoodehnia, K.; Chen, A. A.; Kahl, D.; Komatsubara, T.; José, J.; Longland, R.; Abe, Y.; Binh, D. N.; Chen, J.; Cherubini, S.; Clark, J. A.; Deibel, C. M.; Fukuoka, S.; Hashimoto, T.; Hayakawa, T.; Hendriks, J.; Ishibashi, Y.; Ito, Y.; Kubono, S.; Lennard, W. N.; Moriguchi, T.; Nagae, D.; Nishikiori, R.; Niwa, T.; Ozawa, A.; Parker, P. D.; Seiler, D.; Shizuma, T.; Suzuki, H.; Wrede, C.; Yamaguchi, H.; Yuasa, T.

    2013-06-01

    Background: The uncertainty in the 29P(p,γ)30S reaction rate over 0.1 ≤ T ≤ 1.3 GK was previously determined to span approximately four orders of magnitude due to the uncertain location of two previously unobserved 3+ and 2+ resonances in the Ex=4.7-4.8 MeV region in 30S. Therefore, the abundances of silicon isotopes synthesized in novae, which are relevant for the identification of presolar grains of putative nova origin, were uncertain by a factor of 3.Purpose: (a) To investigate the level structure of 30S above the proton threshold [4394.9(7) keV] via charged-particle spectroscopy using the 32S(p,t)30S reaction and in-beam γ-ray spectroscopy using the 28Si(3He, nγ)30S reaction to calculate the 29P(p,γ)30S reaction rate. (b) To explore the impact of this rate on the abundances of silicon isotopes synthesized in novae.Methods: Differential cross sections of the 32S(p,t)30S reaction were measured at 34.5 MeV. Distorted-wave Born approximation calculations were performed to constrain the spin-parity assignments of the observed levels, including the two astrophysically important levels. An energy-level scheme was deduced from γ-γ coincidence measurements using the 28Si(3He, nγ)30S reaction. Spin-parity assignments based on measurements of γ-ray angular distributions and γ-γ directional correlation from oriented nuclei were made for most of the observed levels of 30S.Results: The resonance energies corresponding to the states with 4.5 MeV ≲ Ex ≲ 6 MeV, including the two astrophysically important states predicted previously, are measured with significantly better precision than before. The spin-parity assignments of both astrophysically important resonances are confirmed. The uncertainty in the rate of the 29P(p,γ)30S reaction is substantially reduced over the temperature range of interest. Finally, the influence of this rate on the abundance ratios of silicon isotopes synthesized in novae are obtained via 1D hydrodynamic nova simulations

  10. {sup 30}S Beam Development and X-ray Bursts

    SciTech Connect

    Kahl, D.; Kubono, S.; Binh, D. N.; Hashimoto, T.; Hayakawa, S.; Kurihara, Y.; Ohshiro, Y.; Yamaguchi, H.; Chen, A. A.; Chen, J.; Setoodeh nia, K.; Kaji, D.; Nishimura, S.; Kim, A.; Lee, N. H.; Wakabayashi, Y.

    2010-03-01

    Over the past three years, we have worked on developing a well-characterized {sup 30}S radioactive beam to be used in a future experiment aiming to directly measure to extrapolate the {sup 30}S(alpha,p) stellar reaction rate within the Gamow window of Type I X-ray bursts. The importance of the {sup 30}S(alpha,p) reaction to X-ray bursts is discussed. Given the astrophysical motivation, the successful results of and challenges involved in the production of a low-energy {sup 30}S beam are detailed. Finally, an overview of our future plans regarding this on-going project are presented.

  11. An assembly landscape for the 30S ribosomal subunit.

    PubMed

    Talkington, Megan W T; Siuzdak, Gary; Williamson, James R

    2005-12-01

    Self-assembling macromolecular machines drive fundamental cellular processes, including transcription, messenger RNA processing, translation, DNA replication and cellular transport. The ribosome, which carries out protein synthesis, is one such machine, and the 30S subunit of the bacterial ribosome is the preeminent model system for biophysical analysis of large RNA-protein complexes. Our understanding of 30S assembly is incomplete, owing to the challenges of monitoring the association of many components simultaneously. Here we have developed a method involving pulse-chase monitored by quantitative mass spectrometry (PC/QMS) to follow the assembly of the 20 ribosomal proteins with 16S ribosomal RNA during formation of the functional particle. These data represent a detailed and quantitative kinetic characterization of the assembly of a large multicomponent macromolecular complex. By measuring the protein binding rates at a range of temperatures, we find that local transformations throughout the assembling subunit have similar but distinct activation energies. Thus, the prevailing view of 30S assembly as a pathway proceeding through a global rate-limiting conformational change must give way to one in which the assembly of the complex traverses a landscape dotted with various local conformational transitions.

  12. An assembly landscape for the 30S ribosomal subunit

    PubMed Central

    Talkington, Megan W. T.; Siuzdak, Gary

    2005-01-01

    Self-assembling macromolecular machines drive fundamental cellular processes, including transcription, mRNA processing, translation, DNA replication, and cellular transport. The ribosome, which carries out protein synthesis, is one such machine, and the 30S subunit of the bacterial ribosome is the preeminent model system for biophysical analysis of large RNA-protein complexes. Our understanding of 30S assembly is incomplete, due to the challenges of monitoring the association of many components simultaneously. We have developed a new method involving pulse-chase monitored by quantitative mass spectrometry (PC/QMS) to follow the assembly of the 20 ribosomal proteins with 16S rRNA during formation of the functional particle. These data represent the first detailed and quantitative kinetic characterization of the assembly of a large multicomponent macromolecular complex. By measuring the protein binding rates at a range of temperatures, we have found that local transformations throughout the assembling subunit have similar but distinct activation energies. This observation shows that the prevailing view of 30S assembly as a pathway proceeding through a global rate-limiting conformational change must give way to a view in which the assembly of the complex traverses a landscape dotted with a variety of local conformational transitions. PMID:16319883

  13. Molecular mechanics of 30S subunit head rotation

    PubMed Central

    Mohan, Srividya; Donohue, John Paul; Noller, Harry F.

    2014-01-01

    During ribosomal translocation, a process central to the elongation phase of protein synthesis, movement of mRNA and tRNAs requires large-scale rotation of the head domain of the small (30S) subunit of the ribosome. It has generally been accepted that the head rotates by pivoting around the neck helix (h28) of 16S rRNA, its sole covalent connection to the body domain. Surprisingly, we observe that the calculated axis of rotation does not coincide with the neck. Instead, comparative structure analysis across 55 ribosome structures shows that 30S head movement results from flexing at two hinge points lying within conserved elements of 16S rRNA. Hinge 1, although located within the neck, moves by straightening of the kinked helix h28 at the point of contact with the mRNA. Hinge 2 lies within a three-way helix junction that extends to the body through a second, noncovalent connection; its movement results from flexing between helices h34 and h35 in a plane orthogonal to the movement of hinge 1. Concerted movement at these two hinges accounts for the observed magnitudes of head rotation. Our findings also explain the mode of action of spectinomycin, an antibiotic that blocks translocation by binding to hinge 2. PMID:25187561

  14. Real-time assembly landscape of bacterial 30S translation initiation complex.

    PubMed

    Milón, Pohl; Maracci, Cristina; Filonava, Liudmila; Gualerzi, Claudio O; Rodnina, Marina V

    2012-05-06

    Initiation factors guide the ribosome in the selection of mRNA and translational reading frame. We determined the kinetically favored assembly pathway of the 30S preinitiation complex (30S PIC), an early intermediate in 30S initiation complex formation in Escherichia coli. IF3 and IF2 are the first factors to arrive, forming an unstable 30S-IF2-IF3 complex. Subsequently, IF1 joins and locks the factors in a kinetically stable 30S PIC to which fMet-tRNA(fMet) is recruited. Binding of mRNA is independent of initiation factors and can take place at any time during 30S PIC assembly, depending on the cellular concentration of the mRNA and the structural determinants at the ribosome-binding site. The kinetic analysis shows both specific and cumulative effects of initiation factors as well as kinetic checkpoints of mRNA selection at the entry into translation.

  15. Insights into structure and function of 30S Ribosomal Protein S2 (30S2) in Chlamydophila pneumoniae: A potent target of pneumonia.

    PubMed

    Koteswara Reddy, G; Nagamalleswara Rao, K; Yarrakula, Kiran

    2017-02-01

    The gene 30S ribosomal protein S2 (30S2) is identified as a potential drug and vaccine target for Pneumonia. Its structural characterization is an important to understand the mechanism of action for identifying its receptor and/or other binding partners. The comparative genomics and proteomics studies are useful for structural characterization of 30S2 in C. Pneumoniae using different bioinformatics tools and web servers. In this study, the protein 30S2 structure was modelled and validated by Ramachandran plot. It is found that the modelled protein under most favoured "core" region was 88.7% and overall G-factor statistics with average score was -0.20. However, seven sequential motifs have been identified for 30S2 with reference codes (PR0095, PF0038, TIGR01012, PTHR11489, SSF52313 and PTHR11489). In addition, seven structural highly conserved residues have been identified in the large cleft are Lys160, Gly161and Arg162 with volume 1288.83Å(3) and average depth of the cleft was 10.75Å. Moreover, biological functions, biochemical process and structural constituents of ribosome are also explored. The study will be helped us to understand the sequential, structural, functional and evolutionary clues of unknown proteins available in C. Pneumoniae.

  16. Finite pure integer programming algorithms employing only hyperspherically deduced cuts

    NASA Technical Reports Server (NTRS)

    Young, R. D.

    1971-01-01

    Three algorithms are developed that may be based exclusively on hyperspherically deduced cuts. The algorithms only apply, therefore, to problems structured so that these cuts are valid. The algorithms are shown to be finite.

  17. {sup 30}S({alpha}, p) in X-Ray Bursts at CRIB

    SciTech Connect

    Kahl, D.; Kubono, S.; Binh, D. N.; Hashimoto, T.; Hayakawa, S.; Kurihara, Y.; Ohshiro, Y.; Yamaguchi, H.; Chen, A. A.; Chen, J.; Setoodeh nia, K.; Kaji, D.; Nishimura, S.; Kim, A.; Lee, N. H.; Wakabayashi, Y.

    2010-08-12

    Over the past three years, we have worked on developing a well-characterized {sup 30}S radioactive beam to be used in a future experiment aiming to directly measure the {sup 30}S({alpha}, p) stellar reaction rate within the Gamow window of Type I X-ray bursts.

  18. Deducing Reaction Mechanism: A Guide for Students, Researchers, and Instructors

    ERIC Educational Resources Information Center

    Meek, Simon J.; Pitman, Catherine L.; Miller, Alexander J. M.

    2016-01-01

    An introductory guide to deducing the mechanism of chemical reactions is presented. Following a typical workflow for probing reaction mechanism, the guide introduces a wide range of kinetic and mechanistic tools. In addition to serving as a broad introduction to mechanistic analysis for students and researchers, the guide has also been used by…

  19. Deducing Reaction Mechanism: A Guide for Students, Researchers, and Instructors

    ERIC Educational Resources Information Center

    Meek, Simon J.; Pitman, Catherine L.; Miller, Alexander J. M.

    2016-01-01

    An introductory guide to deducing the mechanism of chemical reactions is presented. Following a typical workflow for probing reaction mechanism, the guide introduces a wide range of kinetic and mechanistic tools. In addition to serving as a broad introduction to mechanistic analysis for students and researchers, the guide has also been used by…

  20. Goniometer-based femtosecond X-ray diffraction of mutant 30S ribosomal subunit crystals

    DOE PAGES

    Dao, E. Han; Sierra, Raymond G.; Laksmono, Hartawan; ...

    2015-04-30

    In this work, we collected radiation-damage-free data from a set of cryo-cooled crystals for a novel 30S ribosomal subunit mutant using goniometer-based femtosecond crystallography. Crystal quality assessment for these samples was conducted at the X-ray Pump Probe end-station of the Linac Coherent Light Source (LCLS) using recently introduced goniometer-based instrumentation. These 30S subunit crystals were genetically engineered to omit a 26-residue protein, Thx, which is present in the wild-type Thermus thermophilus 30S ribosomal subunit. We are primarily interested in elucidating the contribution of this ribosomal protein to the overall 30S subunit structure. To assess the viability of this study, femtosecondmore » X-ray diffraction patterns from these crystals were recorded at the LCLS during a protein crystal screening beam time. During our data collection, we successfully observed diffraction from these difficult-to-grow 30S ribosomal subunit crystals. Most of our crystals were found to diffract to low resolution, while one crystal diffracted to 3.2 Å resolution. These data suggest the feasibility of pursuing high-resolution data collection as well as the need to improve sample preparation and handling in order to collect a complete radiation-damage-free data set using an X-ray Free Electron Laser.« less

  1. Goniometer-based femtosecond X-ray diffraction of mutant 30S ribosomal subunit crystals

    SciTech Connect

    Dao, E. Han; Sierra, Raymond G.; Laksmono, Hartawan; Lemke, Henrik T.; Alonso-Mori, Roberto; Coey, Aaron; Larsen, Kevin; Baxter, Elizabeth L.; Cohen, Aina E.; Soltis, S. Michael; DeMirci, Hasan

    2015-04-30

    In this work, we collected radiation-damage-free data from a set of cryo-cooled crystals for a novel 30S ribosomal subunit mutant using goniometer-based femtosecond crystallography. Crystal quality assessment for these samples was conducted at the X-ray Pump Probe end-station of the Linac Coherent Light Source (LCLS) using recently introduced goniometer-based instrumentation. These 30S subunit crystals were genetically engineered to omit a 26-residue protein, Thx, which is present in the wild-type Thermus thermophilus 30S ribosomal subunit. We are primarily interested in elucidating the contribution of this ribosomal protein to the overall 30S subunit structure. To assess the viability of this study, femtosecond X-ray diffraction patterns from these crystals were recorded at the LCLS during a protein crystal screening beam time. During our data collection, we successfully observed diffraction from these difficult-to-grow 30S ribosomal subunit crystals. Most of our crystals were found to diffract to low resolution, while one crystal diffracted to 3.2 Å resolution. These data suggest the feasibility of pursuing high-resolution data collection as well as the need to improve sample preparation and handling in order to collect a complete radiation-damage-free data set using an X-ray Free Electron Laser.

  2. Goniometer-based femtosecond X-ray diffraction of mutant 30S ribosomal subunit crystals.

    PubMed

    Dao, E Han; Sierra, Raymond G; Laksmono, Hartawan; Lemke, Henrik T; Alonso-Mori, Roberto; Coey, Aaron; Larsen, Kevin; Baxter, Elizabeth L; Cohen, Aina E; Soltis, S Michael; DeMirci, Hasan

    2015-07-01

    In this work, we collected radiation-damage-free data from a set of cryo-cooled crystals for a novel 30S ribosomal subunit mutant using goniometer-based femtosecond crystallography. Crystal quality assessment for these samples was conducted at the X-ray Pump Probe end-station of the Linac Coherent Light Source (LCLS) using recently introduced goniometer-based instrumentation. These 30S subunit crystals were genetically engineered to omit a 26-residue protein, Thx, which is present in the wild-type Thermus thermophilus 30S ribosomal subunit. We are primarily interested in elucidating the contribution of this ribosomal protein to the overall 30S subunit structure. To assess the viability of this study, femtosecond X-ray diffraction patterns from these crystals were recorded at the LCLS during a protein crystal screening beam time. During our data collection, we successfully observed diffraction from these difficult-to-grow 30S ribosomal subunit crystals. Most of our crystals were found to diffract to low resolution, while one crystal diffracted to 3.2 Å resolution. These data suggest the feasibility of pursuing high-resolution data collection as well as the need to improve sample preparation and handling in order to collect a complete radiation-damage-free data set using an X-ray Free Electron Laser.

  3. Rescue of endogenous 30S retroviral sequences from mouse cells by baboon type C virus.

    PubMed Central

    Sherwin, S A; Rapp, U R; Benveniste, R E; Sen, A; Todaro, G J

    1978-01-01

    Mus musculus SC-1 cells were infected with M7 baboon type C virus. The progeny of this infection included viral pseudotypes that contained M7 helper virus and endogenous 30S retrovirus-associated sequences derived from SC-1 cells (RAS). The RAS sequences are unrelated by nucleic acid hybridization criteria to previously described types of murine retroviruses and do not code for known murine viral structural proteins. The RAS genome is present in multiple copies in the DNA of laboratory (M. musculus) and Asian (M. caroli and M. cervicolor) mice, is expressed in the RNA of uninfected mouse cells, and can be efficiently rescued by type C, but not type B, viruses. RAS is closely related to 30S virus-associated RNA in NIH/3T3 and BALB/c JLSV-9 cells and may be analogous to the defective 30S RNA sequences found in rats. PMID:207887

  4. The effect of ribosome assembly cofactors on in vitro 30S subunit reconstitution.

    PubMed

    Bunner, Anne E; Nord, Stefan; Wikström, P Mikael; Williamson, James R

    2010-04-23

    Ribosome biogenesis is facilitated by a growing list of assembly cofactors, including helicases, GTPases, chaperones, and other proteins, but the specific functions of many of these assembly cofactors are still unclear. The effect of three assembly cofactors on 30S ribosome assembly was determined in vitro using a previously developed mass-spectrometry-based method that monitors the rRNA binding kinetics of ribosomal proteins. The essential GTPase Era caused several late-binding proteins to bind rRNA faster when included in a 30S reconstitution. RimP enabled faster binding of S9 and S19 and inhibited the binding of S12 and S13, perhaps by blocking those proteins' binding sites. RimM caused proteins S5 and S12 to bind dramatically faster. These quantitative kinetic data provide important clues about the roles of these assembly cofactors in the mechanism of 30S biogenesis. (c) 2010 Elsevier Ltd. All rights reserved.

  5. General Constraints on Cross Sections Deduced from Surrogate Reactions

    SciTech Connect

    Younes, W

    2003-08-14

    Cross sections that cannot be measured in the laboratory, e.g. because the target lifetime is too short, can be inferred indirectly from a different reaction forming the same compound system, but with a more accessible beam/target combination (the ''surrogate-reaction'' technique). The reactions share the same compound system and a common decay mechanism, but they involve different formation processes. Therefore, an implicit constraint is imposed on the inferred cross section deduced from the measured surrogate-reaction data, through the common decay mechanism. In this paper, the mathematical consequences of this implicit constraint are investigated. General formulas are derived from upper and lower bounds on the inferred cross section, estimated from surrogate data in a procedure which does not require any modeling of the common decay process. As an example, the formulas developed here are applied to the case of the {sup 235}U(n,f) cross section, deduced from {sup 234}U(t,pf) surrogate data. The calculated bounds are not very tight in this particular case. However, by introducing a few qualitative assumptions about the physics of the fission process, meaningful bounds on the deduced cross section are obtained. Upper and lower limits for the cross-section ratio of the (n,f) reaction on the {sup 235}U isomer at E{sub x} = 77 eV relative to the (n,f) reaction on the ground state are also calculated. The generalization of this technique to other surrogate reactions is discussed.

  6. Relationship between anaerobic capacity estimated using a single effort and 30-s tethered running outcomes

    PubMed Central

    Miyagi, Willian Eiji; Sousa, Filipe Antônio de Barros; Gobatto, Claudio Alexandre

    2017-01-01

    The purpose of the current study was to investigate the relationship between alternative anaerobic capacity method (MAODALT) and a 30-s all-out tethered running test. Fourteen male recreational endurance runners underwent a graded exercise test, a supramaximal exhaustive effort and a 30-s all-out test on different days, interspaced by 48h. After verification of data normality (Shapiro-Wilk test), the Pearson’s correlation test was used to verify the association between the anaerobic estimates from the MAODALT and the 30-s all-out tethered running outputs. Absolute MAODALT was correlated with mean power (r = 0.58; P = 0.03), total work (r = 0.57; P = 0.03), and mean force (r = 0.79; P = 0.001). In addition, energy from the glycolytic pathway (E[La-]) was correlated with mean power (r = 0.58; P = 0.03). Significant correlations were also found at each 5s interval between absolute MAODALT and force values (r between 0.75 and 0.84), and between force values and E[La-] (r between 0.73 to 0.80). In conclusion, the associations between absolute MAODALT and the mechanical outputs from the 30-s all-out tethered running test evidenced the importance of the anaerobic capacity for maintaining force during the course of time in short efforts. PMID:28182775

  7. Visualizing ribosome biogenesis: parallel assembly pathways for the 30S subunit.

    PubMed

    Mulder, Anke M; Yoshioka, Craig; Beck, Andrea H; Bunner, Anne E; Milligan, Ronald A; Potter, Clinton S; Carragher, Bridget; Williamson, James R

    2010-10-29

    Ribosomes are self-assembling macromolecular machines that translate DNA into proteins, and an understanding of ribosome biogenesis is central to cellular physiology. Previous studies on the Escherichia coli 30S subunit suggest that ribosome assembly occurs via multiple parallel pathways rather than through a single rate-limiting step, but little mechanistic information is known about this process. Discovery single-particle profiling (DSP), an application of time-resolved electron microscopy, was used to obtain more than 1 million snapshots of assembling 30S subunits, identify and visualize the structures of 14 assembly intermediates, and monitor the population flux of these intermediates over time. DSP results were integrated with mass spectrometry data to construct the first ribosome-assembly mechanism that incorporates binding dependencies, rate constants, and structural characterization of populated intermediates.

  8. Recognition of Cognate Transfer RNA by the 30S Ribosomal Subunit

    SciTech Connect

    Ogle, James M.; Brodersen, Ditlev E.; Clemons, William M.; Tarry, Michael J.; Carter, Andrew P.; Ramakrishnan, V.

    2009-10-07

    Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces global domain movements of the 30S subunit and changes in the conformation of the universally conserved and essential bases A1492, A1493, and G530 of 16S RNA. These bases interact intimately with the minor groove of the first two base pairs between the codon and anticodon, thus sensing Watson-Crick base-pairing geometry and discriminating against near-cognate tRNA. The third, or 'wobble,' position of the codon is free to accommodate certain noncanonical base pairs. By partially inducing these structural changes, paromomycin facilitates binding of near-cognate tRNAs.

  9. Neutron scattering and the 30 S ribosomal subunit of E. coli

    SciTech Connect

    Moore, P.B.; Engelman, D.M.; Langer, J.A.; Ramakrishnan, V.R.; Schindler, D.G.; Schoenborn, B.P.; Sillers, I.Y.; Yabuki, S.

    1982-01-01

    This paper reviews the progress made in the study of the internal organization of the 30 S ribosomal subunit of E. coli by neutron scattering since 1975. A map of that particle showing the position of 14 of the subunit's 21 proteins is presented, and the methods currently used for collecting and analyzing such data are discussed. Also discussed is the possibility of extending the interpretation of neutron mapping data beyond the limits practical today. 30 references, 5 figures.

  10. Growth, Persistence, and Desistance of Alcohol Use for At-Risk Men in Their 30s.

    PubMed

    Capaldi, Deborah M; Tiberio, Stacey S; Washburn, Isaac J; Yoerger, Karen; Feingold, Alan

    2015-07-01

    Little is known about heterogeneity in men's drinking behaviors and their related consequences across mid-adulthood, and moreover, whether individual or social factors may predict such differences. This study examined 3 indicators of alcohol use, namely alcohol volume, heavy episodic drinking (HED), and drinking-related problems for men in their 30s. Participants were 197 at-risk men from the Oregon Youth Study assessed 5 times across ages 29 to 38 years. Growth mixture modeling with count outcomes was used to examine unobserved heterogeneity in alcohol trajectories. Associations of latent classes of alcohol users with (i) classes for the other alcohol indicators, (ii) alcohol use by peers and romantic partners, (iii) alcohol classes previously extracted from ages 18 to 29 years, and (iv) past year alcohol use disorder (AUD) diagnostic status at ages 35 to 36 years were examined. A 3-class solution afforded the best fit for each alcohol indicator. Alcohol problems were relatively established in the 30s, with an ascending use class found only for volume. Although relatively few men were in higher classes for all 3 indicators, 45% of the sample was in the highest class on at least 2 indicators of use. Peer drunkenness was a robust predictor of the alcohol classes. Concordance among classes of alcohol users was seen from the 20s to the 30s, with prior desistance likely to be maintained for alcohol volume and HED. AUD diagnoses at ages 35 to 36 years were more common in the higher classes obtained for alcohol volume and alcohol problems. Many men in their 30s engaged in a high volume of alcohol use without frequent engagement in HED, likely relating to continuing alcohol problems. The convergence of men's alcohol use with that of their peers found at younger ages was maintained into early mid-adulthood. Copyright © 2015 by the Research Society on Alcoholism.

  11. Neutron Scattering and the 30 S Ribosomal Subunit of E. Coli

    DOE R&D Accomplishments Database

    Moore, P. B.; Engelman, D. M.; Langer, J. A.; Ramakrishnan, V. R.; Schindler, D. G.; Schoenborn, B. P.; Sillers, I. Y.; Yabuki, S.

    1982-06-01

    This paper reviews the progress made in the study of the internal organization of the 30 S ribosomal subunit of E. coli by neutron scattering since 1975. A map of that particle showing the position of 14 of the subunit's 21 proteins is presented, and the methods currently used for collecting and analyzing such data are discussed. Also discussed is the possibility of extending the interpretation of neutron mapping data beyond the limits practical today.

  12. Growth, Persistence, and Desistance of Alcohol Use for At-Risk Men in Their 30s

    PubMed Central

    Capaldi, Deborah M.; Tiberio, Stacey S.; Washburn, Isaac J.; Yoerger, Karen; Feingold, Alan

    2015-01-01

    Background Little is known about heterogeneity in men's drinking behaviors and their related consequences across midadulthood, and moreover, whether individual or social factors may predict such differences. The present study examined 3 indicators of alcohol use; namely, alcohol volume, heavy episodic drinking (HED), and drinking-related problems for men in their 30s. Methods Participants were 197 at-risk men from the Oregon Youth Study assessed 5 times across ages 29–38 years. Growth mixture modeling with count outcomes was used to examine unobserved heterogeneity in alcohol trajectories. Associations of latent classes of alcohol users with (i) classes for the other alcohol indicators, (ii) alcohol use by peers and romantic partners, (iii) alcohol classes previously extracted from ages 18–29 years, and (iv) past year alcohol use disorder (AUD) diagnostic status at ages 35–36 years was examined. Results A 3-class solution afforded the best fit for each alcohol indicator. Alcohol problems were relatively established in the 30s, with an ascending use class found only for volume. Although relatively few men were in higher classes for all 3 indicators, 45% of the sample was in the highest class on at least 2 indicators of use. Peer drunkenness was a robust predictor of the alcohol classes. Concordance among classes of alcohol users was seen from the 20s to the 30s, with prior desistance likely to be maintained for alcohol volume and HED. AUD diagnoses at ages 35–36 years were more common in the higher classes obtained for alcohol volume and alcohol problems. Conclusions Many men in their 30s engaged in high volume of alcohol without frequent engagement in HED, likely relating to continuing alcohol problems. The convergence of men's alcohol use with that of their peers found at younger ages was maintained into early midadulthood. PMID:26010338

  13. Differential assembly of 16S rRNA domains during 30S subunit formation.

    PubMed

    Xu, Zhili; Culver, Gloria M

    2010-10-01

    Rapid and accurate assembly of the ribosomal subunits, which are responsible for protein synthesis, is required to sustain cell growth. Our best understanding of the interaction of 30S ribosomal subunit components (16S ribosomal RNA [rRNA] and 20 ribosomal proteins [r-proteins]) comes from in vitro work using Escherichia coli ribosomal components. However, detailed information regarding the essential elements involved in the assembly of 30S subunits still remains elusive. Here, we defined a set of rRNA nucleotides that are critical for the assembly of the small ribosomal subunit in E. coli. Using an RNA modification interference approach, we identified 54 nucleotides in 16S rRNA whose modification prevents the formation of a functional small ribosomal subunit. The majority of these nucleotides are located in the head and interdomain junction of the 30S subunit, suggesting that these regions are critical for small subunit assembly. In vivo analysis of specific identified sites, using engineered mutations in 16S rRNA, revealed defective protein synthesis capability, aberrant polysome profiles, and abnormal 16S rRNA processing, indicating the importance of these residues in vivo. These studies reveal that specific segments of 16S rRNA are more critical for small subunit assembly than others, and suggest a hierarchy of importance.

  14. Concurrent Nucleation of 16S Folding and Induced Fit in 30S Ribosome Assembly

    SciTech Connect

    Adilakshmi, T.; Bellur, D; Woodson, S

    2008-01-01

    Rapidly growing cells produce thousands of new ribosomes each minute, in a tightly regulated process that is essential to cell growth. How the Escherichia coli 16S ribosomal RNA and the 20 proteins that make up the 30S ribosomal subunit can assemble correctly in a few minutes remains a challenging problem, partly because of the lack of real-time data on the earliest stages of assembly. By providing snapshots of individual RNA and protein interactions as they emerge in real time, here we show that 30S assembly nucleates concurrently from different points along the rRNA. Time-resolved hydroxyl radical footprinting3 was used to map changes in the structure of the rRNA within 20 milliseconds after the addition of total 30S proteins. Helical junctions in each domain fold within 100 ms. In contrast, interactions surrounding the decoding site and between the 5', the central and the 3' domains require 2-200 seconds to form. Unexpectedly, nucleotides contacted by the same protein are protected at different rates, indicating that initial RNA-protein encounter complexes refold during assembly. Although early steps in assembly are linked to intrinsically stable rRNA structure, later steps correspond to regions of induced fit between the proteins and the rRNA.

  15. 30S(α , p) Thermonuclear Reaction Rate from Experimental Level Structure of 34Ar

    NASA Astrophysics Data System (ADS)

    Kahl, D.; Chen, A. A.; Kubono, S.; Yamaguchi, H.; Binh, D. N.; Chen, J.; Cherubini, S.; Duy, N. N.; Hashimoto, T.; Hayakawa, S.; Iwasa, N.; Jung, H. S.; Kato, S.; Kwon, Y. K.; Nishimura, S.; Ota, S.; Setoodehnia, K.; Teranishi, T.; Tokieda, H.; Yamada, T.; Yun, C. C.; Zhang, L. Y.

    Type I X-ray bursts are the most frequent thermonuclear explosions in the galaxy. Owing to their recurrence from known astronomical objects, burst morphology is extensively documented, and they are modeled very successfully as neutron-deficient, thermonuclear runaway on the surface of accreting neutron stars. While reaction networks include hundreds of isotopes and thousands of nuclear processes, only a small subset appear to play a pivotal role. One such reaction is the 30S(α , p) reaction, which is believed to be a crucial link in the explosive helium burning which is responsible for the large energy flux. However, very little experimental information is available concerning the cross section itself, nor the 34Ar compound nucleus at the relevant energies. We performed the first study of the entrance channel via 30S alpha resonant elastic scattering using a state-of-the-art, low-energy, 30S radioactive ion beam. The measurement was performed in inverse kinematics using a newly-developed active target. An R-matrix analysis of the excitation function reveals previously unknown resonances, including their quantum properties of spin, parity, width, and energy.

  16. Crystal Structure of the 30S Ribosomal Subunit from Thermus Thermophilus. Purification, Crystallization and Structure Determination

    SciTech Connect

    Clemons, William M.; Brodersen, Ditlev E.; McCutcheonn, John P.; May, Joanna L.C.; Carter, Andrew P.; Morgan-Warren, Robert J.; Wimberly, Brian T.; Ramakrishnan, Venki

    2009-10-07

    We describe the crystallization and structure determination of the 30 S ribosomal subunit from Thermus thermophilus. Previous reports of crystals that diffracted to 10 {angstrom} resolution were used as a starting point to improve the quality of the diffraction. Eventually, ideas such as the addition of substrates or factors to eliminate conformational heterogeneity proved less important than attention to detail in yielding crystals that diffracted beyond 3 {angstrom} resolution. Despite improvements in technology and methodology in the last decade, the structure determination of the 30 S subunit presented some very challenging technical problems because of the size of the asymmetric unit, crystal variability and sensitivity to radiation damage. Some steps that were useful for determination of the atomic structure were: the use of anomalous scattering from the LIII edges of osmium and lutetium to obtain the necessary phasing signal; the use of tunable, third-generation synchrotron sources to obtain data of reasonable quality at high resolution; collection of derivative data precisely about a mirror plane to preserve small anomalous differences between Bijvoet mates despite extensive radiation damage and multi-crystal scaling; the pre-screening of crystals to ensure quality, isomorphism and the efficient use of scarce third-generation synchrotron time; pre-incubation of crystals in cobalt hexaammine to ensure isomorphism with other derivatives; and finally, the placement of proteins whose structures had been previously solved in isolation, in conjunction with biochemical data on protein-RNA interactions, to map out the architecture of the 30 S subunit prior to the construction of a detailed atomic-resolution model.

  17. Optimal loads for a 30-s maximal power cycle ergometer test using a stationary start.

    PubMed

    Vargas, Nicole T; Robergs, Robert A; Klopp, Dawn M

    2015-05-01

    A stationary start modification to the Wingate Anaerobic Test (WAnT) has become increasingly common. The aim of the present study was to determine whether the traditional 85 g kg(-1) body weight (BW) load (TRAD), or an individualized optimal load (OPT), is more suitable for obtaining peak and mean power outputs (PPO and MPO, respectively) for a stationary start. Twelve recreationally active males and 10 females (mean age 30 ± 9.1 and 25 ± 5.5 years, respectively) completed three trials. The first determined the OPT load and included a familiarization of the 30-s stationary start test, followed by two randomized sessions testing the OPT and TRAD loads during the 30-s stationary start test on separate days. For each test, measures of power (watts), time, and cadence were collected to determine PPO, MPO, rate of power decline (rPD) and time to peak power (TtPP). All power data were corrected for flywheel moment of inertia. Results revealed significant differences between OPT and TRAD load settings for males (95.1 ± 10.7 and 85.06 ± 0.40 g kg(-1) BW; p = 0.008) but not for females (84.71 ± 8.72 and 85.2 ± 0.61 g kg(-1) BW; p = 0.813). Relative PPO was not different for OPT or TRAD loads for males (p = 0.485) or females (p = 0.488). It is not necessary to use an OPT load setting to acquire maximal PO for a 30-s cycle test using a stationary start. Instead, the traditional 85 g kg(-1) BW loading is suitable for both males and females.

  18. Recovery of power output and muscle metabolites following 30 s of maximal sprint cycling in man.

    PubMed Central

    Bogdanis, G C; Nevill, M E; Boobis, L H; Lakomy, H K; Nevill, A M

    1995-01-01

    1. The recovery of power output and muscle metabolites was examined following maximal sprint cycling exercise. Fourteen male subjects performed two 30 s cycle ergometer sprints separated by 1.5, 3 and 6 min of recovery, on three separate occasions. On a fourth occasion eight of the subjects performed only one 30 s sprint and muscle biopsies were obtained during recovery. 2. At the end of the 30 s sprint phosphocreatine (PCr) and ATP contents were 19.7 +/- 1.2 and 70.5 +/- 6.5% of the resting values (rest), respectively, while muscle lactate was 119.0 +/- 4.6 mmol (kg dry wt)-1 and muscle pH was 6.72 +/- 0.06. During recovery, PCr increased rapidly to 65.0 +/- 2.8% of rest after 1.5 min, but reached only 85.5 +/- 3.5% of rest after 6 min of recovery. At the same time ATP and muscle pH remained low (19.5 +/- 0.9 mmol (kg dry wt)-1 and 6.79 +/- 0.02, respectively). Modelling of the individual PCr resynthesis using a power function curve gave an average half-time for PCr resynthesis of 56.6 +/- 7.3 s. 3. Recovery of peak power output (PPO), peak pedal speed (maxSp) and mean power during the initial 6 s (MPO6) of sprint 2 did not reach the control values after 6 min of rest, and occurred in parallel with the resynthesis of PCr, despite the low muscle pH. High correlations (r = 0.71-0.86; P < 0.05) were found between the percentage resynthesis of PCr and the percentage restoration of PPO, maxSp and MPO6 after 1.5 and 3 min of recovery. No relationship was observed between muscle pH recovery and power output restoration during sprint 2 (P > 0.05). 4. These data suggest that PCr resynthesis after 30 s of maximal sprint exercise is slower than previously observed after dynamic exercise of longer duration, and PCr resynthesis is important for the recovery of power during repeated bouts of sprint exercise. Images Figure 1 PMID:7714837

  19. Kinematics of ICMEs Deduced From Remote Radio Observations

    NASA Astrophysics Data System (ADS)

    Reiner, M. J.; MacDowall, R. J.

    2009-12-01

    Low-frequency radio emissions, generated at the driven shock wave at the fundamental and harmonic of the plasma frequency, can directly reveal the kinematics of ICMEs as they propagate through the inner heliosphere. The reason is that the frequency of the radio emissions varies in a predictable way as a function of heliocentric distance. Hence, the observed frequency drift of these radio emissions is essentially a plot of the height above the Sun as a function of time. The derivative of the observed frequency-time curve at each point then gives the instantaneous speed of the propagating interplanetary shock. We have used these remote radio observations to determine the speed profiles for some 40 fast CMEs observed during solar cycle 23. The speed profiles for these fast ICMEs were found to imply an initial rapid deceleration at a constant rate, followed by a constant propagation speed to 1 AU (Reiner et al. ApJ 663, 1369, 2007), consistent with some earlier Doppler scintillation measurements (Woo et al., JGR 90, 154, 1985). Because of the large number of CME events for which this analysis was carried out, we were further able to study the correlations of the deceleration parameters of the ICME speed profiles. For most of those remote radio observations, there were no corresponding white-light observations beyond the 32 Rs (0.15 AU) limit of the LASCO coronagraph. After 2003, the all-sky camera SMEI permitted the first direct comparison between the remote radio and the white-light observations in interplanetary space (Reiner et al. JGR 110, A09S14, 2005). The STEREO spacecraft, launched in October of 2006, provide a new and unique opportunity to make direct comparisons between the radio and white-light observations of the ICME kinematics. The STEREO observations also allow the locations of the radio sources along the shock front to be directly deduced from two or three spacecraft triangulation measurement from STEREO and Wind (Reiner et al. Solar Physics 10.1007/s

  20. Antimicrobial efficacy of alcohol-based hand gels with a 30-s application.

    PubMed

    do Prado, M F; Coelho, A C C; de Brito, J P B; Ferreira, D O; Junior, A W; Menecucci, C da Silva; de Queiroz, A B; Garcia, L B; Cardoso, C L; Tognim, M C B

    2012-06-01

    The objective of this study was to assess the antimicrobial efficacy of alcohol-based hand gels according to European Norm 1500 (EN 1500). We assessed the antimicrobial efficacy of 12 alcohol-based hand gels produced in Brazil, containing 70% w/w or v/v ethyl alcohol as the active ingredient, according to EN 1500, with a 30-s application. In addition, 70% w/w ethyl alcohol and three alcohol-based hand rubs commonly used in Europe and effective according to EN 1500 were also tested. Eight of 12 (67%) alcohol-based hand gels produced in Brazil failed by EN 1500. In contrast, 70% w/w ethyl alcohol and European alcohol-based hand rubs were approved by EN 1500. In this study, the majority of Brazilian alcohol-based hand gels showed limited efficacy on hand hygiene within 30 s. The findings of this study may be used as an important argument to motivate Brazilian manufacturers to improve the antimicrobial efficacy of alcohol-based hand gels, because it is prudent to suppose that alcohol-based hand gels can be recommended for use in healthcare settings only if they show antimicrobial activity at least similar to that of alcohol-based liquid preparations, including the traditional 70% w/w ethyl alcohol. © 2012 The Authors. Letters in Applied Microbiology © 2012 The Society for Applied Microbiology.

  1. Changes in muscle metabolites in females with 30-s exhaustive exercise.

    PubMed

    Jacobs, I; Bar-Or, O; Karlsson, J; Dotan, R; Tesch, P; Kaiser, P; Inbar, O

    1982-01-01

    The purpose of this study was to quantify the changes in selected intramuscular metabolites associated with non-oxidative energy metabolism after performance of the Wingate Test (WT), a widely used, exhaustive, 30-s cycle test of short-time muscular power. Muscle biopsies were taken from the m. vastus lateralis of nine female physical education students at rest and immediately after performance of the WT. The concentrations of adenosine triphosphate (ATP), creatine phosphate (CP), lactate, and glycogen were determined. The ATP decreased from 20.9 to 13.8; CP decreased from 62.7 to 25.1; lactate increased from 9.0 to 60.5; and glycogen decreased from 360 to 278 (all concentrations are mmol X kg-1 dry muscle). The absolute changes in CP and lactate were not as large as those reported in other exercise studies. Based on the metabolite changes, it was concluded that the WT is a satisfactory test of the maximal muscular power that can be generated from non-oxidative metabolism, but that the 30-s duration of the test probably does not tax the maximal capacity of such energy metabolism.

  2. Does power indicate capacity? 30-s Wingate anaerobic test vs. maximal accumulated O2 deficit.

    PubMed

    Minahan, C; Chia, M; Inbar, O

    2007-10-01

    The purpose of this study was to evaluate the relationship between anaerobic power and capacity. Seven men and seven women performed a 30-s Wingate Anaerobic Test on a cycle ergometer to determine peak power, mean power, and the fatigue index. Subjects also cycled at a work rate predicted to elicit 120 % of peak oxygen uptake to exhaustion to determine the maximal accumulated O (2) deficit. Peak power and the maximal accumulated O (2) deficit were significantly correlated (r = 0.782, p = 0.001). However, when the absolute difference in exercise values between groups (men and women) was held constant using a partial correlation, the relationship diminished (r = 0.531, p = 0.062). In contrast, we observed a significant correlation between fatigue index and the maximal accumulated O (2) deficit when controlling for gender (r = - 0.597, p = 0.024) and the relationship remained significant when values were expressed relative to active muscle mass. A higher anaerobic power does not indicate a greater anaerobic capacity. Furthermore, we suggest that the ability to maintain power output during a 30-s cycle sprint is related to anaerobic capacity.

  3. Deducing spectroscopic factors from wave-function asymptotics

    SciTech Connect

    Capel, P.; Danielewicz, P.; Nunes, F. M.

    2010-11-15

    In a coupled-channel model, we explore the effects of coupling between configurations on the radial behavior of the wave function and, in particular, on the spectroscopic factor (SF) and the asymptotic normalization coefficient (ANC). We evaluate the extraction of a SF from the ratio of the ANC of the coupled-channel model to that of a single-particle approximation of the wave function. We perform this study within a core+n collective model, which includes two states of the core that connect by a rotational coupling. To get additional insights, we also use a simplified model that takes a {delta} function for the coupling potential. Calculations are performed for {sup 11}Be. Fair agreement is obtained between the SF inferred from the single-particle approximation and the one obtained within the coupled-channel models. Significant discrepancies are observed only for large coupling strength and/or large admixture, that is, a small SF. This suggests that reliable SFs can be deduced from the wave-function asymptotics when the structure is dominated by one configuration, that is, for a large SF.

  4. Characteristic views of E. coli and B. stearothermophilus 30S ribosomal subunits in the electron microscope.

    PubMed Central

    van Heel, M; Stöffler-Meilicke, M

    1985-01-01

    Large sets of electron microscopic images of the 30S ribosomal subunits of Bacillus stearothermophilus (914 molecules) and Escherichia coli (422 molecules) were analysed with image processing techniques. Using computer alignment and a new multivariate statistical classification scheme, three predominant views of the subunit were found for both species. These views, which together account for approximately 90% of the population of images, were determined to a reproducible resolution of up to 1.7 nm, thus elucidating many new structural details. The angular spread of the molecular orientations around the three main stable positions is remarkably small (less than 8 degrees). Some of the current models for the small ribosomal subunit are incompatible with our new results. Images Fig. 1. Fig. 2. Fig. 3. Fig. 4. Fig. 5. Fig. 6. PMID:3908096

  5. The aminoglycoside resistance methyltransferases from the ArmA/Rmt family operate late in the 30S ribosomal biogenesis pathway.

    PubMed

    Zarubica, Tamara; Baker, Matthew R; Wright, H Tonie; Rife, Jason P

    2011-02-01

    Bacterial resistance to 4,6-type aminoglycoside antibiotics, which target the ribosome, has been traced to the ArmA/RmtA family of rRNA methyltransferases. These plasmid-encoded enzymes transfer a methyl group from S-adenosyl-L-methionine to N7 of the buried G1405 in the aminoglycoside binding site of 16S rRNA of the 30S ribosomal subunit. ArmA methylates mature 30S subunits but not 16S rRNA, 50S, or 70S ribosomal subunits or isolated Helix 44 of the 30S subunit. To more fully characterize this family of enzymes, we have investigated the substrate requirements of ArmA and to a lesser extent its ortholog RmtA. We determined the Mg+² dependence of ArmA activity toward the 30S ribosomal subunits and found that the enzyme recognizes both low Mg+² (translationally inactive) and high Mg+² (translationally active) forms of this substrate. We tested the effects of LiCl pretreatment of the 30S subunits, initiation factor 3 (IF3), and gentamicin/kasugamycin resistance methyltransferase (KsgA) on ArmA activity and determined whether in vivo derived pre-30S ribosomal subunits are ArmA methylation substrates. ArmA failed to methylate the 30S subunits generated from LiCl washes above 0.75 M, despite the apparent retention of ribosomal proteins and a fully mature 16S rRNA. From our experiments, we conclude that ArmA is most active toward the 30S ribosomal subunits that are at or very near full maturity, but that it can also recognize more than one form of the 30S subunit.

  6. Empirical singular vectors of baroclinic flows deduced from experimental data

    NASA Astrophysics Data System (ADS)

    Harlander, Uwe; Hoff, Michael; Seelig, Torsten; Egbers, Christoph

    2013-04-01

    Instability is related to exponentially growing eigenmodes. Interestingly, when finite time intervals are considered, growth rates of certain initial perturbations can exceed the growth rates of the most unstable modes. Moreover, even when all modes are damped, such particular initial perturbations can grow dramatically during finite time intervals. The perturbations with the largest growth rates are called singular vectors or optimal perturbations. They not only play an important role in atmospheric ensemble predictions, but also for the theory of instability and turbulence. Starting point for the singular vector analysis is a linear dynamical system with a known system matrix. In atmospheric ensemble prediction, linearization is generally done around a nonlinear solution and then the system matrix is time-dependent. In contrast, in turbulence research linearization is done around a mean state. In that case the system matrix is time-independent. We consider the mathematically simpler latter case here. We decompose surface temperature data of a differentially heated rotating annulus experiment into principal oscillation patterns, that are the empirically estimated linear eigenmodes of the system. Using these modes we can estimate the modes of maximal growth, which are thus the empirically estimated singular vectors. In contrast to the traditional approach we deduce these modes from the data alone without using a linear model operator and thus without actually knowing the system matrix (Penland and Sardeshmukh, 1995). In our study we will address the following questions: What is the appropriate filter technique to remove noise from the data prior to the empirical singular vector analysis? Is there an objective mean to distinguish between noise and signal? How accurate are the empirical singular vectors? To answer the last question we compare the data based results with findings from a numerical model for which the system matrix is known. We want to test the idea

  7. Conformational Response of 30S-bound IF3 to A-Site Binders Streptomycin and Kanamycin.

    PubMed

    Chulluncuy, Roberto; Espiche, Carlos; Nakamoto, Jose Alberto; Fabbretti, Attilio; Milón, Pohl

    2016-12-13

    Aminoglycoside antibiotics are widely used to treat infectious diseases. Among them, streptomycin and kanamycin (and derivatives) are of importance to battle multidrug-resistant (MDR) Mycobacterium tuberculosis. Both drugs bind the small ribosomal subunit (30S) and inhibit protein synthesis. Genetic, structural, and biochemical studies indicate that local and long-range conformational rearrangements of the 30S subunit account for this inhibition. Here, we use intramolecular FRET between the C- and N-terminus domains of the flexible IF3 to monitor real-time perturbations of their binding sites on the 30S platform. Steady and pre-steady state binding experiments show that both aminoglycosides bring IF3 domains apart, promoting an elongated state of the factor. Binding of Initiation Factor IF1 triggers closure of IF3 bound to the 30S complex, while both aminoglycosides revert the IF1-dependent conformation. Our results uncover dynamic perturbations across the 30S subunit, from the A-site to the platform, and suggest that both aminoglycosides could interfere with prokaryotic translation initiation by modulating the interaction between IF3 domains with the 30S platform.

  8. Conformational Response of 30S-bound IF3 to A-Site Binders Streptomycin and Kanamycin

    PubMed Central

    Chulluncuy, Roberto; Espiche, Carlos; Nakamoto, Jose Alberto; Fabbretti, Attilio; Milón, Pohl

    2016-01-01

    Aminoglycoside antibiotics are widely used to treat infectious diseases. Among them, streptomycin and kanamycin (and derivatives) are of importance to battle multidrug-resistant (MDR) Mycobacterium tuberculosis. Both drugs bind the small ribosomal subunit (30S) and inhibit protein synthesis. Genetic, structural, and biochemical studies indicate that local and long-range conformational rearrangements of the 30S subunit account for this inhibition. Here, we use intramolecular FRET between the C- and N-terminus domains of the flexible IF3 to monitor real-time perturbations of their binding sites on the 30S platform. Steady and pre-steady state binding experiments show that both aminoglycosides bring IF3 domains apart, promoting an elongated state of the factor. Binding of Initiation Factor IF1 triggers closure of IF3 bound to the 30S complex, while both aminoglycosides revert the IF1-dependent conformation. Our results uncover dynamic perturbations across the 30S subunit, from the A-site to the platform, and suggest that both aminoglycosides could interfere with prokaryotic translation initiation by modulating the interaction between IF3 domains with the 30S platform. PMID:27983590

  9. Switching off the tackiness of a nanocomposite adhesive in 30 s via infrared sintering.

    PubMed

    Gurney, Robert S; Dupin, Damien; Nunes, Juliana S; Ouzineb, Keltoum; Siband, Elodie; Asua, José M; Armes, Steven P; Keddie, Joseph L

    2012-10-24

    Soft adhesives require an optimum balance of viscous and elastic properties. Adhesion is poor when the material is either too solidlike or too liquidlike. The ability to switch tack adhesion off at a desired time has many applications, such as in recycling, disassembly of electronics, and painless removal of wound dressings. Here, we describe a new strategy to switch off the tack adhesion in a model nanocomposite adhesive in which temperature is the trigger. The nanocomposite comprises hard methacrylic nanoparticles blended with a colloidal dispersion of soft copolymer particles. At relatively low volume fractions, the nanoparticles (50 nm diameter) accumulate near the film surface, where they pack around the larger soft particles (270 nm). The viscoelasticity of the nanocomposite is adjusted via the nanoparticle concentration. When the nanocomposite is heated above the glass transition temperature of the nanoparticles (T(g) = 130 °C), they sinter together to create a rigid network that raises the elastic modulus at room temperature. The tackiness is switched off. Intense infrared radiation is used to heat the nanocomposites, leading to a fast temperature rise. Tack adhesion is switched off within 30 s in optimized compositions. These one-way switchable adhesives have the potential to be patterned through localized heating.

  10. Mesoscale Wind Regimes In The Elqui-valley/chile At 30 S

    NASA Astrophysics Data System (ADS)

    Kalthoff, N.; Bischoff-Gauß, I.; Fiebig-Wittmaack, M.; Fiedler, F.; Thürauf, J.; Novoa, E.; Pizarro, C.; Castillo, R.; Kohler, M.

    In November 1999, four permanent surface stations were installed in the vicinity of the surface ozone monitoring station on the summit of the Cerro Tololo (2200 m amsl) at 30 S. These stations were used to study the atmospheric flow conditions which are important for the interpretation of the ozone measurements at Cerro Tololo. Addi- tionally, radiosonde ascents were performed in March 2000 near the coast and about 60 km inland. Different wind regimes were distinguished. Above 4 km, large-scale westerly winds prevailed, while northerly winds were observed in a band along the coastline between 2 km and 4 km height. The upper boundary of the northerly wind regime corresponded with the mean height of the Andes. This wind regime resulted from the westerly winds being blocked and forced to flow parallel to the Andes (when Froude number Fr < 1). The phenomenon was also confirmed by model simulations. Seasonally varying thermally induced valley winds and a sea breeze developed be- low the northerly wind regime. In summer, the valley winds reached the Cerro Tololo. Diurnal variation of the top of the valley winds also influenced the lower boundary of the northerly wind regime, which was less than 2 km amsl during the night and greater than 2 km amsl during the day. Thus, this observational and modeling study has shown that in summer the baseline ozone monitoring site at Cerro Tololo can be contaminated by polluted air that is transported from the plains by the thermally induced wind systems.

  11. Muscle fibre conduction velocity during a 30-s Wingate anaerobic test.

    PubMed

    Stewart, David; Farina, Dario; Shen, Chao; Macaluso, Andrea

    2011-06-01

    Ten male volunteers (age 29.2 ± 5.2 years, mean ± SD) were recruited to test the hypothesis that muscle fibre conduction velocity (MFCV) would decrease with power output during a 30-s Wingate test on a mechanically-braked cycle ergometer. Prior to the main test, the optimal pre-fixed load corresponding to the highest power output was selected following a random series of six 10-s sprints. Surface electromyographic (EMG) signals were detected from the right vastus lateralis with linear adhesive arrays of eight electrodes. Power output decreased significantly from 6-s until the end of the test (860.9 ± 207.8 vs. 360.9 ± 11.4 W, respectively) and was correlated with MFCV (R=0.543, P<0.01), which also declined significantly by 26.8 ± 11% (P<0.05). There was a tendency for the mean frequency of the EMG power spectrum (MNF) to decrease, but average rectified values (ARV) remained unchanged throughout the test. The parallel decline of MFCV with power output suggests changes in fibre membrane properties. The unaltered ARV, together with the declined MFCV, would indicate either a decrease in discharge rate, de-recruitment of fatigued motor units or elongation of still present motor unit action potentials.

  12. Relative Contribution of Arms and Legs in 30 s Fully Tethered Front Crawl Swimming.

    PubMed

    Morouço, Pedro G; Marinho, Daniel A; Izquierdo, Mikel; Neiva, Henrique; Marques, Mário C

    2015-01-01

    The relative contribution of arm stroke and leg kicking to maximal fully tethered front crawl swimming performance remains to be solved. Twenty-three national level young swimmers (12 male and 11 female) randomly performed 3 bouts of 30 s fully tethered swimming (using the whole body, only the arm stroke, and only the leg kicking). A load-cell system permitted the continuous measurement of the exerted forces, and swimming velocity was calculated from the time taken to complete a 50 m front crawl swim. As expected, with no restrictions swimmers were able to exert higher forces than that using only their arm stroke or leg kicking. Estimated relative contributions of arm stroke and leg kicking were 70.3% versus 29.7% for males and 66.6% versus 33.4% for females, with 15.6% and 13.1% force deficits, respectively. To obtain higher velocities, male swimmers are highly dependent on the maximum forces they can exert with the arm stroke (r = 0.77, P < 0.01), whereas female swimmers swimming velocity is more related to whole-body mean forces (r = 0.81, P < 0.01). The obtained results point that leg kicking plays an important role over short duration high intensity bouts and that the used methodology may be useful to identify strength and/or coordination flaws.

  13. Assembly of the 30S ribosomal subunit: positioning ribosomal protein S13 in the S7 assembly branch.

    PubMed

    Grondek, Joel F; Culver, Gloria M

    2004-12-01

    Studies of Escherichia coli 30S ribosomal subunit assembly have revealed a hierarchical and cooperative association of ribosomal proteins with 16S ribosomal RNA; these results have been used to compile an in vitro 30S subunit assembly map. In single protein addition and omission studies, ribosomal protein S13 was shown to be dependent on the prior association of ribosomal protein S20 for binding to the ribonucleoprotein particle. While the overwhelming majority of interactions revealed in the assembly map are consistent with additional data, the dependency of S13 on S20 is not. Structural studies position S13 in the head of the 30S subunit > 100 A away from S20, which resides near the bottom of the body of the 30S subunit. All of the proteins that reside in the head of the 30S subunit, except S13, have been shown to be part of the S7 assembly branch, that is, they all depend on S7 for association with the assembling 30S subunit. Given these observations, the assembly requirements for S13 were investigated using base-specific chemical footprinting and primer extension analysis. These studies reveal that S13 can bind to 16S rRNA in the presence of S7, but not S20. Additionally, interaction between S13 and other members of the S7 assembly branch have been observed. These results link S13 to the 3' major domain family of proteins, and the S7 assembly branch, placing S13 in a new location in the 30S subunit assembly map where its position is in accordance with much biochemical and structural data.

  14. Divergent marijuana trajectories among men: Socioeconomic, relationship, and life satisfaction outcomes in the mid-30s.

    PubMed

    White, Helene R; Bechtold, Jordan; Loeber, Rolf; Pardini, Dustin

    2015-11-01

    Given recent changes in marijuana policy in the United States, it is important to understand the long-term effects of marijuana use on adult functioning. We examined whether men who displayed different trajectories of marijuana use from adolescence through emerging adulthood (age ∼15-26) differed in terms of socioeconomic, social, and life satisfaction outcomes in their mid-30s. Data came from a longitudinal sample of men who were recruited in early adolescence (N=506) and followed into adulthood. Four trajectory groups based on patterns of marijuana use from adolescence into emerging adulthood were compared on adult outcomes (age ∼36) before and after controlling for co-occurring use of other substances and several pre-existing confounding factors in early adolescence. The potential moderating effect of race was also examined. Although there were initially group differences across all domains, once pre-existing confounds and co-occurring other substance use were included in the model, groups only differed in terms of partner and friend marijuana use. Chronic marijuana users reported the highest proportions of both. Frequent and persistent marijuana use was associated with lower socioeconomic status (SES) for Black men only. After statistically accounting for confounding variables, chronic marijuana users were not at a heightened risk for maladjustment in adulthood except for lower SES among Black men. Chronic users were more likely to have friends and partners who also used marijuana. Future studies should take into account pre-existing differences when examining outcomes of marijuana use. Copyright © 2015. Published by Elsevier Ireland Ltd.

  15. Divergent marijuana trajectories among men: Socioeconomic, relationship, and life satisfaction outcomes in the mid-30s

    PubMed Central

    White, Helene R.; Bechtold, Jordan; Loeber, Rolf; Pardini, Dustin

    2016-01-01

    Background Given recent changes in marijuana policy in the United States, it is important to understand the long-term effects of marijuana use on adult functioning. We examined whether men who displayed different trajectories of marijuana use from adolescence through emerging adulthood (age ~15–26) differed in terms of socioeconomic, social, and life satisfaction outcomes in their mid-30s. Methods Data came from a longitudinal sample of men who were recruited in early adolescence (N = 506) and followed into adulthood. Four trajectory groups based on patterns of marijuana use from adolescence into emerging adulthood were compared on adult outcomes (age ~36) before and after controlling for co-occurring use of other substances and several pre-existing confounding factors in early adolescence. The potential moderating effect of race was also examined. Results Although there were initially group differences across all domains, once pre-existing confounds and co-occurring other substance use were included in the model, groups only differed in terms of partner and friend marijuana use. Chronic marijuana users reported the highest proportions of both. Frequent and persistent marijuana use was associated with lower socioeconomic status (SES) for Black men only. Conclusions After statistically accounting for confounding variables, chronic marijuana users were not at a heightened risk for maladjustment in adulthood except for lower SES among Black men. Chronic users were more likely to have friends and partners who also used marijuana. Future studies should take into account pre-existing differences when examining outcomes of marijuana use. PMID:26365837

  16. Quantitation of ten 30S ribosomal assembly intermediates using fluorescence triple correlation spectroscopy.

    PubMed

    Ridgeway, William K; Millar, David P; Williamson, James R

    2012-08-21

    The self-assembly of bacterial 30S ribosomes involves a large number of RNA folding and RNA-protein binding steps. The sequence of steps determines the overall assembly mechanism and the structure of the mechanism has ramifications for the robustness of biogenesis and resilience against kinetic traps. Thermodynamic interdependencies of protein binding inferred from omission-reconstitution experiments are thought to preclude certain assembly pathways and thus enforce ordered assembly, but this concept is at odds with kinetic data suggesting a more parallel assembly landscape. A major challenge is deconvolution of the statistical distribution of intermediates that are populated during assembly at high concentrations approaching in vivo assembly conditions. To specifically resolve the intermediates formed by binding of three ribosomal proteins to the full length 16S rRNA, we introduce Fluorescence Triple-Correlation Spectroscopy (F3CS). F3CS identifies specific ternary complexes by detecting coincident fluctuations in three-color fluorescence data. Triple correlation integrals quantify concentrations and diffusion kinetics of triply labeled species, and F3CS data can be fit alongside auto-correlation and cross-correlation data to quantify the populations of 10 specific ribosome assembly intermediates. The distribution of intermediates generated by binding three ribosomal proteins to the entire native 16S rRNA included significant populations of species that were not previously thought to be thermodynamically accessible, questioning the current interpretation of the classic omission-reconstitution experiments. F3CS is a general approach for analyzing assembly and function of macromolecular complexes, especially those too large for traditional biophysical methods.

  17. Cross-validation of the 20- versus 30-s Wingate anaerobic test.

    PubMed

    Laurent, C Matthew; Meyers, Michael C; Robinson, Clay A; Green, J Matt

    2007-08-01

    The 30-s Wingate anaerobic test (30-WAT) is the most widely accepted protocol for measuring anaerobic response, despite documented physical side effects. Abbreviation of the 30-WAT without loss of data could enhance subject compliance while maintaining test applicability. The intent of this study was to quantify the validity of the 20-s Wingate anaerobic test (20-WAT) versus the traditional 30-WAT. Fifty males (mean +/- SEM; age = 20.5 +/- 0.3 years; Ht = 1.6 +/- 0.01 m; Wt = 75.5 +/- 2.6 kg) were randomly selected to either a validation (N = 35) or cross-validation group (N = 15) and completed a 20-WAT and 30-WAT in double blind, random order on separate days to determine peak power (PP; W kg(-1)), mean power (MP; W kg(-1)), and fatigue index (FI; %). Utilizing power outputs (relative to body mass) recorded during each second of both protocols, a non-linear regression equation (Y (20WAT+10 )= 31.4697 e(-0.5)[ln(X (second)/1174.3961)/2.6369(2)]; r (2) = 0.97; SEE = 0.56 W kg(-1)) successfully predicted (error approximately 10%) the final 10 s of power outputs in the cross-validation population. There were no significant differences between MP and FI between the 20-WAT that included the predicted 10 s of power outputs (20-WAT+10) and the 30-WAT. When derived data were subjected to Bland-Altman analyses, the majority of plots (93%) fell within the limits of agreement (+/-2SD). Therefore, when compared to the 30-WAT, the 20-WAT may be considered a valid alternative when used with the predictive non-linear regression equation to derive the final power output values.

  18. Analysis of conformational changes in 16 S rRNA during the course of 30 S subunit assembly.

    PubMed

    Holmes, Kristi L; Culver, Gloria M

    2005-11-25

    Ribosome biogenesis involves an integrated series of binding events coupled with conformational changes that ultimately result in the formation of a functional macromolecular complex. In vitro, Escherichia coli 30 S subunit assembly occurs in a cooperative manner with the ordered addition of 20 ribosomal proteins (r-proteins) with 16 S rRNA. The assembly pathway for 30 S subunits has been dissected in vitro into three steps, where specific r-proteins associate with 16 S rRNA early in 30 S subunit assembly, followed by a mid-assembly conformational rearrangement of the complex that then enables the remaining r-proteins to associate in the final step. Although the three steps of 30 S subunit assembly have been known for some time, few details have been elucidated about changes that occur as a result of these three specific stages. Here, we present a detailed analysis of the concerted early and late stages of small ribosomal subunit assembly. Conformational changes, roles for base-pairing and r-proteins at specific stages of assembly, and a polar nature to the assembly process have been revealed. This work has allowed a more comprehensive and global view of E.coli 30 S ribosomal subunit assembly to be obtained.

  19. PPARA intron polymorphism associated with power performance in 30-s anaerobic Wingate Test.

    PubMed

    Petr, Miroslav; Stastny, Petr; Št'astný, Petr; Pecha, Ondřej; Šteffl, Michal; Šeda, Ondřej; Kohlíková, Eva

    2014-01-01

    To date, polymorphisms in several genes have been associated with a strength/power performance including alpha 3 actinin, ciliary neurotrophic factor, vitamin D receptor, or angiotensin I converting enzyme, underlining the importance of genetic component of the multifactorial strength/power-related phenotypes. The single nucleotide variation in peroxisome proliferator-activated receptor alpha gene (PPARA) intron 7 G/C (rs4253778; g.46630634G>C) has been repeatedly found to play a significant role in response to different types of physical activity. We investigated the effect of PPARA intron 7 G/C polymorphism specifically on anaerobic power output in a group of 77 elite male Czech ice hockey players (18-36 y). We determined the relative peak power per body weight (Pmax.kg(-1)) and relative peak power per fat free mass (W.kg(-1)FFM) during the 30-second Wingate Test (WT30) on bicycle ergometer (Monark 894E Peak bike, MONARK, Sweden). All WT30s were performed during the hockey season. Overall genotype frequencies were 50.6% GG homozygotes, 40.3% CG heterozygotes, and 9.1% CC homozygotes. We found statistically significant differences in Pmax.kg(-1) and marginally significant differences in Pmax.kg(-1)FFM values in WT30 between carriers and non-carriers for C allele (14.6 ± 0.2 vs. 13.9 ± 0.3 W.kg(-1) and 15.8 ± 0.2 vs. 15.2 ± 0.3 W.kg(-1)FFM, P = 0.036 and 0.12, respectively). Furthermore, Pmax.kg(-1)FFM strongly positively correlated with the body weight only in individuals with GG genotypes (R = 0.55; p<0.001). Our results indicate that PPARA 7C carriers exhibited higher speed strength measures in WT30. We hypothesize that C allele carriers within the cohort of trained individuals may possess a metabolic advantage towards anaerobic metabolism.

  20. Advance of the perihelion of Mercury deduced from QFT

    NASA Astrophysics Data System (ADS)

    Chen, Shao-Guang

    I deduce the new gravitational formula from the variance in mass of QFT and GR (H05-0029-08, E15-0039 -08, E14-0032-08, D31-0054-10) in the partial differential: f (QFT) = f (GR) = delta∂ (m v)/delta∂ t = f _{P} + f _{C} , f _{P} = m delta∂ v / delta∂ t = - ( G m M /r (2) ) r / r, f _{C} = v delta∂ m / delta∂ t = - ( G m M / r (2) ) v / c (1), f (QFT) is the quasi-Casimir pressure of net virtual neutrinos nuν _{0} flux (after counteract contrary direction nuν _{0}). f (GR) is equivalent to Einstein’s equation, Eq. (1) is a new version of GR and can be solved exactly. Its core content is that the gravity produced by particles collide cannot linear addition, i.e., the nonlinearity of Einstein equation had been replaced by the nonlinearity caused by the variable mass in Eq.(1). Einstein equation can be inferred from Eq.(1) thereby from QFT, but QFT cannot be inferred from Eq.(1) or GR. f (QFT) is essential but f (GR) is phenomenological. Eq.(1) is obtained just by to absorb the essence of corpuscule collided gravitation origin ism proposed by Fatio in 1690 and 1920 Majorana’s experiment concept about gravitational shield effect again fuse with QFT. In my paper ‘QFT’S advance of the perihelion of Mercury, China Science &Technology Overview 125 88-90 (2011)’ QFT gravitational potential U = - G M /r is just the distribution density of net nuν _{0} flux, from SR we again get that: f (QFT) = f _{P} + f _{C}, f _{P} = - m ( delta∂ U / delta∂ r) r / r, f _{C} = - m ( delta∂U / delta∂ r) v / c (2), f _{ P} correspond the change rate of three-dimensional momentum p, f _{C} correspond the change rate of fourth dimensional momentum i m c which show directly as a dissipative force of mass change. According to Eq.(2) the circular motion is instability and elliptic motion is in the auto-stability state. In the fluctuation vacuum a particle with mass M neighbor another particle with mass m, the renormalization mass M and m will be less than that when

  1. In vivo X-ray footprinting of pre-30S ribosomes reveals chaperone-dependent remodeling of late assembly intermediates.

    PubMed

    Clatterbuck Soper, Sarah F; Dator, Romel P; Limbach, Patrick A; Woodson, Sarah A

    2013-11-21

    Assembly of 30S ribosomal subunits from their protein and RNA components requires extensive refolding of the 16S rRNA and is assisted by 10-20 assembly factors in bacteria. We probed the structures of 30S assembly intermediates in E. coli cells, using a synchrotron X-ray beam to generate hydroxyl radical in the cytoplasm. Widespread differences between mature and pre-30S complexes in the absence of assembly factors RbfA and RimM revealed global reorganization of RNA-protein interactions prior to maturation of the 16S rRNA and showed how RimM reduces misfolding of the 16S 3' domain during transcription in vivo. Quantitative (14)N/(15)N mass spectrometry of affinity-purified pre-30S complexes confirmed the absence of tertiary assembly proteins and showed that N-terminal acetylation of proteins S18 and S5 correlates with correct folding of the platform and central pseudoknot. Our results indicate that cellular factors delay specific RNA folding steps to ensure the quality of assembly. Copyright © 2013 Elsevier Inc. All rights reserved.

  2. Developmental Trajectories of Marijuana Use among Men: Examining Linkages with Criminal Behavior and Psychopathic Features into the Mid-30s

    PubMed Central

    Pardini, Dustin; Bechtold, Jordan; Loeber, Rolf; White, Helene

    2015-01-01

    Objectives Examine whether young men who chronically use marijuana are at risk for engaging in drug-related and non-drug-related criminal offending and exhibiting psychopathic personality features in their mid-30s. Methods Patterns of marijuana use were delineated in a sample of predominately Black and White young men from adolescence to the mid-20s using latent class growth curve analysis. Self-report and official records of criminal offending and psychopathic personality features were assessed in the mid-30s. Analyses controlled for multiple factors indicative of a preexisting antisocial lifestyle and co-occurring use of other substances and tested for moderation by race. Results Four latent marijuana trajectory groups were identified: chronic high, adolescence-limited, late increasing, and low/nonusers. Relative to low/nonusers, chronic high and late increasing marijuana users exhibited more adult psychopathic features and were more likely to engage in drug-related offending during their mid-30s. Adolescence-limited users were similar to low/nonusers in terms of psychopathic features but were more likely to be arrested for drug-related crimes. No trajectory group differences were found for violence or theft, and the group differences were not moderated by race. Conclusions Young men who engage in chronic marijuana use from adolescence into their 20s are at increased risk for exhibiting psychopathic features, dealing drugs, and enduring drug-related legal problems in their mid-30s relative to men who remain abstinent or use infrequently. PMID:26568641

  3. New evidence for the antiquity of man in North America deduced from aspartic acid racemization.

    PubMed

    Bada, J L; Schroeder, R A; Carter, G F

    1974-05-17

    Ages of several Californzia Paleo-Indlian skeletons have been deduced from the extent of aspartic acid racemization. These dates suggest that man was present in North America at least 50,000 years before the present.

  4. Differences in Prevalence of Metabolic Syndrome by Breastfeeding Experience of Women in Their 30s and 40s.

    PubMed

    Kim, Hye-Jin; Kim, Hee-Seung

    2016-06-01

    The purpose of this study was to assess the differences in the prevalence of metabolic syndrome in women in their 30s and 40s by breastfeeding experience, using the the fifth Korea National Health and Nutrition Examination Survey (2010) data. In this cross-sectional study, a total of 1,053 healthy women in their 30s and 40s, who had given birth was analyzed. To compare women with and without breastfeeding experience, chi-square test and t test were used. The relationship between metabolic syndrome and breastfeeding was assessed using logistic regression analysis adjusted demographic and lifestyle covariates. The breastfeeding experience of women in their 30s was associated with a decreased risk of elevated triglyceride after controlling for income, education, exercise and the last childbirth age [odds ratio (OR) = 0.44, 95% confidence interval (CI) (0.21, 0.95)]. In addition, women who breastfed more children had high odds of metabolic syndrome [OR = 4.03, 95%CI (2.03, 8.00)], and components of metabolic syndrome [abdominal obesity: OR = 2.02, 95%CI (1.17, 3.51), elevated triglyceride: OR = 1.98, 95%CI (1.14, 3.45), elevated blood pressure: OR = 2.65, 95%CI (1.28, 5.49)] than those who never breastfed children. This study found that postpartum breastfeeding may play a significant role in reducing the risk of metabolic syndrome and also that childbearing is associated with a higher incidence of metabolic syndrome among women in their 30s. For women in their 40s, the risk of metabolic syndrome did not significantly differ depending on the breastfeeding experience. This study indicated that breastfeeding can be a way to reduce metabolic health burdens in women in their 30s. Copyright © 2016. Published by Elsevier B.V.

  5. Characterization of the optical constants and dispersion parameters of chalcogenide Te40Se30S30 thin film: thickness effect

    NASA Astrophysics Data System (ADS)

    Abd-Elrahman, M. I.; Hafiz, M. M.; Qasem, Ammar; Abdel-Rahim, M. A.

    2016-02-01

    Chalcogenide Te40Se30S30 thin films of different thickness (100-450 nm) are prepared by thermal evaporation of the Te40Se30S30 bulk. X-ray examination of the film shows some prominent peaks relate to crystalline phases indicating the crystallization process. The calculated particles of crystals from the X-ray diffraction peaks are found to be from 11 to 26 nm. As the thickness increases, the transmittance decreases and the reflectance increases. This could be attributed to the increment of the absorption of photons as more states will be available for absorbance in the case of thicker films. The decrease in the direct band gap with thickness is accompanied with an increase in energy of localized states. The obtained data for the refractive index could be fit to the dispersion model based on the single oscillator equation. The single-oscillator energy decreases, while the dispersion energy increases as the thickness increases.

  6. Single crystal XRD structure and theoretical analysis of the chiral Au30S(S-t-Bu)18 cluster.

    PubMed

    Crasto, David; Malola, Sami; Brosofsky, Grace; Dass, Amala; Häkkinen, Hannu

    2014-04-02

    Au30S(S-t-Bu)18 cluster, related closely to the recently isolated "green gold" compound Au30(S-t-Bu)18, has been structurally solved via single-crystal XRD and analyzed by density functional theory calculations. The molecular protecting layer shows a combination of monomeric (RS-Au-SR) and trimeric (RS-Au-SR-Au-SR-Au-SR) gold-thiolate units, bridging thiolates, and a single sulfur (sulfide) in a novel μ3-coordinating position. The chiral gold core has a geometrical component that is identical to the core of the recently reported Au28(SPh-t-Bu)20. Both enantiomers of Au30S(S-t-Bu)18 are found in the crystal unit cell. The calculated CD spectrum bears a close resemblance to that of Au28(SPh-t-Bu)20. This is the first time when two structurally characterized thiol-stabilized gold clusters are found to have such closely related metal core structures and the results may increase understanding of the formation of gold clusters when stabilized by bulky thiolates.

  7. Wildland fire probabilities estimated from weather model-deduced monthly mean fire danger indices

    Treesearch

    Haiganoush K. Preisler; Shyh-Chin Chen; Francis Fujioka; John W. Benoit; Anthony L. Westerling

    2008-01-01

    The National Fire Danger Rating System indices deduced from a regional simulation weather model were used to estimate probabilities and numbers of large fire events on monthly and 1-degree grid scales. The weather model simulations and forecasts are ongoing experimental products from the Experimental Climate Prediction Center at the Scripps Institution of Oceanography...

  8. CONSISTENT USE OF THE KALMAN FILTER IN CHEMICAL TRANSPORT MODELS (CTMS) FOR DEDUCING EMISSIONS

    EPA Science Inventory

    Past research has shown that emissions can be deduced using observed concentrations of a chemical, a Chemical Transport Model (CTM), and the Kalman filter in an inverse modeling application. An expression was derived for the relationship between the "observable" (i.e., the con...

  9. CONSISTENT USE OF THE KALMAN FILTER IN CHEMICAL TRANSPORT MODELS (CTMS) FOR DEDUCING EMISSIONS

    EPA Science Inventory

    Past research has shown that emissions can be deduced using observed concentrations of a chemical, a Chemical Transport Model (CTM), and the Kalman filter in an inverse modeling application. An expression was derived for the relationship between the "observable" (i.e., the con...

  10. Understanding ribosome assembly: the structure of in vivo assembled immature 30S subunits revealed by cryo-electron microscopy.

    PubMed

    Jomaa, Ahmad; Stewart, Geordie; Martín-Benito, Jaime; Zielke, Ryszard; Campbell, Tracey L; Maddock, Janine R; Brown, Eric D; Ortega, Joaquin

    2011-04-01

    Four decades after early in vitro assembly studies demonstrated that ribosome assembly is a controlled process, our understanding of ribosome assembly is still incomplete. Just as structure determination has been so important to understanding ribosome function, so too will it be critical to sorting out the assembly process. Here, we used a viable deletion in the yjeQ gene, a recognized ribosome assembly factor, to isolate and structurally characterize immature 30S subunits assembled in vivo. These small ribosome subunits contained unprocessed 17S rRNA and lacked some late ribosomal proteins. Cryo-electron microscopy reconstructions revealed that the presence of precursor sequences in the rRNA induces a severe distortion in the 3' minor domain of the subunit involved in the decoding of mRNA and interaction with the large ribosome subunit. These findings suggest that rRNA processing events induce key local conformational changes directing the structure toward the mature assembly. We concluded that rRNA processing, folding, and the entry of tertiary r-proteins are interdependent events in the late stages of 30S subunit assembly. In addition, we demonstrate how studies of emerging assembly factors in ribosome biogenesis can help to elucidate the path of subunit assembly in vivo.

  11. Stable isotope pulse-chase monitored by quantitative mass spectrometry applied to E. coli 30S ribosome assembly kinetics.

    PubMed

    Bunner, Anne E; Williamson, James R

    2009-10-01

    Stable isotope mass spectrometry has become a widespread tool in quantitative biology. Pulse-chase monitored by quantitative mass spectrometry (PC/QMS) is a recently developed stable isotope approach that provides a powerful means of studying the in vitro self-assembly kinetics of macromolecular complexes. This method has been applied to the Escherichia coli 30S ribosomal subunit, but could be applied to any stable self-assembling complex that can be reconstituted from its component parts and purified from a mixture of components and complex. The binding rates of 18 out of the 20 ribosomal proteins have been measured at several temperatures using PC/QMS. Here, PC/QMS experiments on 30S ribosomal subunit assembly are described, and the potential application of the method to other complexes is discussed. A variation on the PC/QMS experiment is introduced that enables measurement of kinetic cooperativity between proteins. In addition, several related approaches to stable isotope labeling and quantitative mass spectrometry data analysis are compared and contrasted.

  12. Heterogeneity in Men’s Marijuana Use in the 20s: Adolescent Antecedents and Consequences in the 30s

    PubMed Central

    Washburn, Isaac J.; Capaldi, Deborah M.

    2014-01-01

    Adolescent psychopathology is commonly connected to marijuana use. How changes in these adolescent antecedents and in adolescent marijuana use are connected to patterns of marijuana use in the 20s is little understood. Also not clearly understood is psychopathology in the 30s as predicted by marijuana use in the 20s. This study sought to examine these two issues – and associations with marijuana disorder diagnoses – using a longitudinal dataset of 205 men with essentially annual reports. Individual psychopathology and family characteristics from the men’s adolescence were used to predict their patterns of marijuana use across their 20s, and aspects of the men’s psychopathology in their mid 30s were predicted from these patterns. Three patterns of marijuana use in the 20s were identified using growth mixture modeling and were associated with diagnoses of marijuana disorders at age 26 years. Parental marijuana use predicted chronic use for the men in adulthood. Patterns of marijuana use in the 20s predicted antisocial behavior and deviant peer association at age 36 years (controlling for adolescent levels of the outcomes by residualization). These findings indicate that differential patterns of marijuana use in early adulthood are associated with psychopathology toward midlife. PMID:25017389

  13. A combined quantitative mass spectrometry and electron microscopy analysis of ribosomal 30S subunit assembly in E. coli.

    PubMed

    Sashital, Dipali G; Greeman, Candacia A; Lyumkis, Dmitry; Potter, Clinton S; Carragher, Bridget; Williamson, James R

    2014-10-14

    Ribosome assembly is a complex process involving the folding and processing of ribosomal RNAs (rRNAs), concomitant binding of ribosomal proteins (r-proteins), and participation of numerous accessory cofactors. Here, we use a quantitative mass spectrometry/electron microscopy hybrid approach to determine the r-protein composition and conformation of 30S ribosome assembly intermediates in Escherichia coli. The relative timing of assembly of the 3' domain and the formation of the central pseudoknot (PK) structure depends on the presence of the assembly factor RimP. The central PK is unstable in the absence of RimP, resulting in the accumulation of intermediates in which the 3'-domain is unanchored and the 5'-domain is depleted for r-proteins S5 and S12 that contact the central PK. Our results reveal the importance of the cofactor RimP in central PK formation, and introduce a broadly applicable method for characterizing macromolecular assembly in cells.

  14. Automatic evaluation of the 30-s chair stand test using inertial/magnetic-based technology in an older prefrail population.

    PubMed

    Millor, Nora; Lecumberri, Pablo; Gomez, Marisol; Martinez-Ramirez, Alicia; Rodriguez-Manas, Leocadio; Garcia-Garcia, Francisco José; Izquierdo, Mikel

    2013-07-01

    The aim of this study was to evaluate the inertial measures of the 30-s chair stand test using modern body-fixed motion sensors. Polynomial data fitting was used to correct the drift effect in the position estimation. Thereafter, the three most important test cycles phases ("impulse," "stand up," and "sit down") were characterized and automatically analyzed. Automated test control is provided, making it possible for researchers without engineering knowledge to run the test. A collection of meaningful data based on kinematic variables is selected for further research. The proposed methodology for data analysis is a feasible tool for use in clinical settings. This method may not only improve rehabilitation therapies but also identify people at risk for falls more accurately than simply evaluating the number of cycles.

  15. [Desperately seeking decentralisation: Mexican health policies in two periods of reform: the 1920s-30s and the 1980s].

    PubMed

    Birn, Anne-Emannuelle

    2005-01-01

    This article compares public health policy reforms in Mexico during the 1920s and 1930s with subsequent reforms initiated in the 1980s. The attempts at decentralization in the 1920s-30s were supported by the Rockefeller Foundation, which was interested in the formation of local cooperative health units. In the 1980s, the aim of the Mexican government and international financial agencies, such as the Inter-American Development Bank, was to reduce public spending (as part of "structural adjustment" policies). One of the hypotheses of this article is that, in the end, the public health reforms were unable to overcome the limitations imposed by Mexico's political centralization and longstanding inequities in public spending. At the same time, one of the unforeseen achievements of these reforms was an increase in local capabilities to demand a better distribution of social services.

  16. Single protein omission reconstitution studies of tetracycline binding to the 30S subunit of Escherichia coli ribosomes

    SciTech Connect

    Buck, M.; Cooperman, B.S. )

    1990-06-05

    In previous work the authors showed that on photolysis of Escherichia coli ribosomes in the presence of ({sup 3}H)tetracycline (TC) the major protein labeled is S7, and they presented strong evidence that such labeling takes place from a high-affinity site related to the inhibitory action of TC. In this work they use single protein omission reconstitution (SPORE) experiments to identify those proteins that are important for high-affinity TC binding to the 30S subunit, as measured by both cosedimentation and filter binding assays. With respect to both sedimentation coefficients and relative Phe-tRNA{sup Phe} binding, the properties of the SPORE particles they obtain parallel very closely those measured earlier, with the exception of the SPORE particle lacking S13. A total of five proteins, S3, S7, S8, S14, and S19, are shown to be important for TC binding, with the largest effects seen on omission of proteins S7 and S14. Determination of the protein compositions of the corresponding SPORE particles demonstrates that the observed effects are, for the most part, directly attributable to the omission of the given protein rather than reflecting an indirect effect of omitting one protein on the uptake of another. A large body of evidence supports the notion that four of these proteins, S3, S7, S14, and S19, are included, along with 16S rRNA bases 920-1,396, in one of the major domains of the 30S subunit. The results support the conclusion that the structure of this domain is important for the binding of TC and that, within this domain, TC binds directly to S7.

  17. Physics-based Inverse Problem to Deduce Marine Atmospheric Boundary Layer Parameters

    DTIC Science & Technology

    2017-03-07

    solution to the model parameters. As such, part of this effort examines the forward problem to improve understanding of the physical mechanisms that...environmental factors including refractivity. Study of the forward problem in this context improves our understanding of the physical mechanisms impacting EM...please find the Final Technical Report with SF 298 for Dr. Erin E. Hackett’s ONR grant entitled Physics -based Inverse Problem to Deduce Marine

  18. The DEDUCE Guided Query Tool: Providing Simplified Access to Clinical Data for Research and Quality Improvement

    PubMed Central

    Horvath, Monica M.; Winfield, Stephanie; Evans, Steve; Slopek, Steve; Shang, Howard; Ferranti, Jeffrey

    2011-01-01

    In many healthcare organizations, comparative effectiveness research and quality improvement (QI) investigations are hampered by a lack of access to data created as a byproduct of patient care. Data collection often hinges upon either manual chart review or ad hoc requests to technical experts who support legacy clinical systems. In order to facilitate this needed capacity for data exploration at our institution (Duke University Health System), we have designed and deployed a robust Web application for cohort identification and data extraction—the Duke Enterprise Data Unified Content Explorer (DEDUCE). DEDUCE is envisioned as a simple, web-based environment that allows investigators access to administrative, financial, and clinical information generated during patient care. By using business intelligence tools to create a view into Duke Medicine's enterprise data warehouse, DEDUCE provides a guided query functionality using a wizard-like interface that lets users filter through millions of clinical records, explore aggregate reports, and, export extracts. Researchers and QI specialists can obtain detailed patient- and observation-level extracts without needing to understand structured query language or the underlying database model. Developers designing such tools must devote sufficient training and develop application safeguards to ensure that patient-centered clinical researchers understand when observation-level extracts should be used. This may mitigate the risk of data being misunderstood and consequently used in an improper fashion. PMID:21130181

  19. The DEDUCE Guided Query tool: providing simplified access to clinical data for research and quality improvement.

    PubMed

    Horvath, Monica M; Winfield, Stephanie; Evans, Steve; Slopek, Steve; Shang, Howard; Ferranti, Jeffrey

    2011-04-01

    In many healthcare organizations, comparative effectiveness research and quality improvement (QI) investigations are hampered by a lack of access to data created as a byproduct of patient care. Data collection often hinges upon either manual chart review or ad hoc requests to technical experts who support legacy clinical systems. In order to facilitate this needed capacity for data exploration at our institution (Duke University Health System), we have designed and deployed a robust Web application for cohort identification and data extraction--the Duke Enterprise Data Unified Content Explorer (DEDUCE). DEDUCE is envisioned as a simple, web-based environment that allows investigators access to administrative, financial, and clinical information generated during patient care. By using business intelligence tools to create a view into Duke Medicine's enterprise data warehouse, DEDUCE provides a Guided Query functionality using a wizard-like interface that lets users filter through millions of clinical records, explore aggregate reports, and, export extracts. Researchers and QI specialists can obtain detailed patient- and observation-level extracts without needing to understand structured query language or the underlying database model. Developers designing such tools must devote sufficient training and develop application safeguards to ensure that patient-centered clinical researchers understand when observation-level extracts should be used. This may mitigate the risk of data being misunderstood and consequently used in an improper fashion.

  20. A combined quantitative mass spectrometry and electron microscopy analysis of ribosomal 30S subunit assembly in E. coli

    PubMed Central

    Sashital, Dipali G; Greeman, Candacia A; Lyumkis, Dmitry; Potter, Clinton S; Carragher, Bridget; Williamson, James R

    2014-01-01

    Ribosome assembly is a complex process involving the folding and processing of ribosomal RNAs (rRNAs), concomitant binding of ribosomal proteins (r-proteins), and participation of numerous accessory cofactors. Here, we use a quantitative mass spectrometry/electron microscopy hybrid approach to determine the r-protein composition and conformation of 30S ribosome assembly intermediates in Escherichia coli. The relative timing of assembly of the 3′ domain and the formation of the central pseudoknot (PK) structure depends on the presence of the assembly factor RimP. The central PK is unstable in the absence of RimP, resulting in the accumulation of intermediates in which the 3′-domain is unanchored and the 5′-domain is depleted for r-proteins S5 and S12 that contact the central PK. Our results reveal the importance of the cofactor RimP in central PK formation, and introduce a broadly applicable method for characterizing macromolecular assembly in cells. DOI: http://dx.doi.org/10.7554/eLife.04491.001 PMID:25313868

  1. Deducing nanopore structure and growth mechanisms in porogen-templated silsesquioxane thin films

    NASA Astrophysics Data System (ADS)

    Peng, Hua-Gen; Vallery, Richard S.; Liu, Ming; Frieze, William E.; Gidley, David W.; Yim, Jin-Heong; Jeong, Hyun-Dam; Kim, Jongmin

    2005-10-01

    Adjusting the functional group of a porogen is found to have a tremendous effect on the pore structre of porous low dielectric constant films with silsesquioxane as the matrix precursor. The pore size and interconnection length measured by positronium annihilation lifetime spectroscopy can be used to deduce the pore shape and its evolution with porosity from templates of isolated porogen molecules through film percolation. Inert, self-linkable, and amphiphilic porogens are demonstrated to randomly aggregate three-dimensionally, linearly polymerize, and form micelles, respectively.

  2. Estimation of uncertainty in isotherms deduced from Hugoniots resulting from shockwave generated defects

    NASA Technical Reports Server (NTRS)

    Ruoff, A. L.

    1980-01-01

    The problem of how defect concentrations produced in a shock experiment affect the isothermal equation of state is considered. From the few experimental results available that bear directly on this question, it appears the effect could be substantial, i.e., the pressure in material free of defects may be 10-25% lower at a given volume than the isothermal pressure deduced from Hugoniot data on the assumption that defects are negligible in the shocked material. This correction caused by the presence of defects is called the defect pressure.

  3. Nonbridging phosphate oxygens in 16S rRNA important for 30S subunit assembly and association with the 50S ribosomal subunit.

    PubMed

    Ghosh, Srikanta; Joseph, Simpson

    2005-05-01

    Ribosomes are composed of RNA and protein molecules that associate together to form a supramolecular machine responsible for protein biosynthesis. Detailed information about the structure of the ribosome has come from the recent X-ray crystal structures of the ribosome and the ribosomal subunits. However, the molecular interactions between the rRNAs and the r-proteins that occur during the intermediate steps of ribosome assembly are poorly understood. Here we describe a modification-interference approach to identify nonbridging phosphate oxygens within 16S rRNA that are important for the in vitro assembly of the Escherichia coli 30S small ribosomal subunit and for its association with the 50S large ribosomal subunit. The 30S small subunit was reconstituted from phosphorothioate-substituted 16S rRNA and small subunit proteins. Active 30S subunits were selected by their ability to bind to the 50S large subunit and form 70S ribosomes. Analysis of the selected population shows that phosphate oxygens at specific positions in the 16S rRNA are important for either subunit assembly or for binding to the 50S subunit. The X-ray crystallographic structures of the 30S subunit suggest that some of these phosphate oxygens participate in r-protein binding, coordination of metal ions, or for the formation of intersubunit bridges in the mature 30S subunit. Interestingly, however, several of the phosphate oxygens identified in this study do not participate in any interaction in the mature 30S subunit, suggesting that they play a role in the early steps of the 30S subunit assembly.

  4. What are the causes for the spread of GLE parameters deduced from NM data?

    NASA Astrophysics Data System (ADS)

    Bütikofer, R.; Flückiger, E.

    2015-08-01

    Investigations have shown that the analysis results of ground level enhancements (GLEs) based on neutron monitor (NM) data for a selected event can differ considerably depending the procedure used. This may have significant consequences e.g. for the assessment of radiation doses at flight altitudes. The reasons for the spread of the GLE parameters deduced from NM data can be manifold and are at present unclear. They include differences in specific properties of the various analysis procedures (e.g. NM response functions, different ways in taking into account the dynamics of the Earth's magnetospheric field), different characterisations of the solar particle flux near Earth as well as the specific selection of NM stations used for the analysis. In the present paper we quantitatively investigate this problem for a time interval during the maximum phase of the GLE on 13 December 2006. We present and discuss the changes in the resulting GLE parameters when using different NM response functions, different model representations of the Earth's magnetospheric field as well as different assumptions for the solar particle spectrum and pitch angle distribution near Earth. The results of the study are expected to yield a basis for the reduction in the spread of the GLE parameters deduced from NM data.

  5. Synthetic oligonucleotide probes deduced from amino acid sequence data. Theoretical and practical considerations.

    PubMed

    Lathe, R

    1985-05-05

    Synthetic probes deduced from amino acid sequence data are widely used to detect cognate coding sequences in libraries of cloned DNA segments. The redundancy of the genetic code dictates that a choice must be made between (1) a mixture of probes reflecting all codon combinations, and (2) a single longer "optimal" probe. The second strategy is examined in detail. The frequency of sequences matching a given probe by chance alone can be determined and also the frequency of sequences closely resembling the probe and contributing to the hybridization background. Gene banks cannot be treated as random associations of the four nucleotides, and probe sequences deduced from amino acid sequence data occur more often than predicted by chance alone. Probe lengths must be increased to confer the necessary specificity. Examination of hybrids formed between unique homologous probes and their cognate targets reveals that short stretches of perfect homology occurring by chance make a significant contribution to the hybridization background. Statistical methods for improving homology are examined, taking human coding sequences as an example, and considerations of codon utilization and dinucleotide frequencies yield an overall homology of greater than 82%. Recommendations for probe design and hybridization are presented, and the choice between using multiple probes reflecting all codon possibilities and a unique optimal probe is discussed.

  6. Nucleotide and deduced amino acid sequences of Torpedo californica acetylcholine receptor gamma subunit.

    PubMed Central

    Claudio, T; Ballivet, M; Patrick, J; Heinemann, S

    1983-01-01

    The nucleotide sequence has been determined of a cDNA clone that codes for the 60,000-dalton gamma subunit of Torpedo californica acetylcholine receptor. The length of the cDNA clone is 2,010 base pairs. The 5' and 3' untranslated regions have respective lengths of 31 and 461 base pairs. Data suggest that the putative polyadenylylation consensus sequence A-A-T-A-A-A may not be required for polyadenylylation of the mRNA corresponding to the cDNA clone described in this study. From the DNA sequence data, the amino acid sequence of the gamma subunit was deduced. The subunit is composed of 489 amino acids giving a molecular mass of 56,600 daltons. The deduced amino acid sequence data also indicate the presence of a 17-amino acid extension or signal peptide on this subunit. From these data, structural predictions for the gamma subunit are made such as potential membrane-spanning regions, possible asparagine-linked glycosylation sites, and the assignment of regions of the protein to the extracellular, internal, and cytoplasmic domains of the lipid bilayer. Images PMID:6573658

  7. Near-Surface Flow Fields Deduced Using Correlation Tracking and Time-Distance Analysis

    NASA Technical Reports Server (NTRS)

    DeRosa, Marc; Duvall, T. L., Jr.; Toomre, Juri

    1999-01-01

    Near-photospheric flow fields on the Sun are deduced using two independent methods applied to the same time series of velocity images observed by SOI-MDI on SOHO. Differences in travel times between f modes entering and leaving each pixel measured using time-distance helioseismology are used to determine sites of supergranular outflows. Alternatively, correlation tracking analysis of mesogranular scales of motion applied to the same time series is used to deduce the near-surface flow field. These two approaches provide the means to assess the patterns and evolution of horizontal flows on supergranular scales even near disk center, which is not feasible with direct line-of-sight Doppler measurements. We find that the locations of the supergranular outflows seen in flow fields generated from correlation tracking coincide well with the locations of the outflows determined from the time-distance analysis, with a mean correlation coefficient after smoothing of bar-r(sub s) = 0.840. Near-surface velocity field measurements can used to study the evolution of the supergranular network, as merging and splitting events are observed to occur in these images. The data consist of one 2048-minute time series of high-resolution (0.6" pixels) line-of-sight velocity images taken by MDI on 1997 January 16-18 at a cadence of one minute.

  8. Near-Surface Flow Fields Deduced Using Correlation Tracking and Time-Distance Analysis

    NASA Technical Reports Server (NTRS)

    DeRosa, Marc; Duvall, T. L., Jr.; Toomre, Juri

    1999-01-01

    Near-photospheric flow fields on the Sun are deduced using two independent methods applied to the same time series of velocity images observed by SOI-MDI on SOHO. Differences in travel times between f modes entering and leaving each pixel measured using time-distance helioseismology are used to determine sites of supergranular outflows. Alternatively, correlation tracking analysis of mesogranular scales of motion applied to the same time series is used to deduce the near-surface flow field. These two approaches provide the means to assess the patterns and evolution of horizontal flows on supergranular scales even near disk center, which is not feasible with direct line-of-sight Doppler measurements. We find that the locations of the supergranular outflows seen in flow fields generated from correlation tracking coincide well with the locations of the outflows determined from the time-distance analysis, with a mean correlation coefficient after smoothing of bar-r(sub s) = 0.840. Near-surface velocity field measurements can used to study the evolution of the supergranular network, as merging and splitting events are observed to occur in these images. The data consist of one 2048-minute time series of high-resolution (0.6" pixels) line-of-sight velocity images taken by MDI on 1997 January 16-18 at a cadence of one minute.

  9. Thermoplastic elastomer with advanced hydrophilization and bonding performances for rapid (30 s) and easy molding of microfluidic devices.

    PubMed

    Lachaux, Julie; Alcaine, Clara; Gómez-Escoda, Blanca; Perrault, Cécile M; Duplan, David Olea; Wu, Pei-Yun Jenny; Ochoa, Iñaki; Fernandez, Luis; Mercier, Olaf; Coudreuse, Damien; Roy, Emmanuel

    2017-07-25

    One of the most important areas of research on microfluidic technologies focuses on the identification and characterisation of novel materials with enhanced properties and versatility. Here we present a fast, easy and inexpensive microstructuration method for the fabrication of novel, flexible, transparent and biocompatible microfluidic devices. Using a simple hot press, we demonstrate the rapid (30 s) production of various microfluidic prototypes embossed in a commercially available soft thermoplastic elastomer (sTPE). This styrenic block copolymer (BCP) material is as flexible as PDMS and as thermoformable as classical thermoplastics. It exhibits high fidelity of replication using SU-8 and epoxy master molds in a highly convenient low-isobar (0.4 bar) and iso-thermal process. Microfluidic devices can then be easily sealed using either a simple hot plate or even a room-temperature assembly, allowing them to sustain liquid pressures of 2 and 0.6 bar, respectively. The excellent sorption and biocompatibility properties of the microchips were validated via a standard rhodamine dye assay as well as a sensitive yeast cell-based assay. The morphology and composition of the surface area after plasma treatment for hydrophilization purposes are stable and show constant and homogenous distribution of block nanodomains (∼22° after 4 days). These domains, which are evenly distributed on the nanoscale, therefore account for the uniform and convenient surface of a "microfluidic scale device". To our knowledge, this is the first thermoplastic elastomer material that can be used for fast and reliable fabrication and assembly of microdevices while maintaining a high and stable hydrophilicity.

  10. The dynamics of distance, velocity and acceleration of power output in the 30-s Wingate Anaerobic Test.

    PubMed

    Bell, W; Cobner, D

    2011-02-01

    The purpose of the present study was to analyse the dynamics of distance, velocity and acceleration of the 30-s Wingate Anaerobic Test. Participants were 53 young adult Rugby Union football players of mean age 21.6±2.5 yr, 180.5±7.2 cm height and 89.3±12.7 kg body mass. Measurements of power were obtained using a friction-belt cycle ergometer (Monark 864, Varberg, Sweden). Individual data were aligned according to peak power output, which resulted in a mean value of 1 216±256 W, compared with one of 1 180±256 W when calculated cross-sectionally (p<0.0001). The derivatives of velocity and acceleration were obtained using the mathematical software Mathcad. Distance, velocity and acceleration curves were plotted simultaneously at 1 s intervals before and after peak power output (-4 s to +28 s). The initial rise of the distance curve was the result of a general trend in decreasing positive velocities as far as peak power output, followed thereafter by a gradual deterioration of power, the result of negative velocities from peak power output to +28 s peak power output. The initial values of the acceleration curve showed a fluctuating decelerating trend of negative values to peak power output; subsequently all values remained positive running along the zero acceleration time axis. Coefficients of correlation between peak power output and power values at -1 s to -3 s were 0.80, 0.65 and 0.63 respectively (p<0.001). The relationship between velocity and acceleration was - 0.968 (p<0.01).

  11. Family Planning and Preconception Health Among Men in Their Mid-30s: Developing Indicators and Describing Need.

    PubMed

    Casey, Frances E; Sonenstein, Freya L; Astone, Nan M; Pleck, Joseph H; Dariotis, Jacinda K; Marcell, Arik V

    2016-01-01

    The Centers for Disease Control and Prevention and Healthy People 2020 call for improvements in meeting men's reproductive health needs but little is known about the proportion of men in need. This study describes men aged 35 to 39 in need of family planning and preconception care, demographic correlates of these needs, and contraception use among men in need of family planning. Using data from Wave 4 (2008-2010) of the National Survey of Adolescent Males, men were classified in need of family planning and preconception care if they reported sex with a female in the last year and believed that they and their partner were fecund; the former included men who were neither intentionally pregnant nor intending future children and the latter included men intending future children. Men were classified as being in need of both if they reported multiple sex partners in the past year. About 40% of men aged 35 to 39 were in need of family planning and about 33% in need of preconception care with 12% in need of both. Current partner's age, current union type, and sexually transmitted infection health risk differentiated men in need of family planning and preconception care (all ps < .01) and participants' race/ethnicity further differentiated men in need of preconception care (p < .01). More than half of men in need of family planning reported none of the time current partner hormonal use (55%) or condom use (52%) during the past year. This study identified that many men in their mid-30s are in need of family planning or preconception care. © The Author(s) 2014.

  12. Three-dimensional placement of the conserved 530 loop of 16 S rRNA and of its neighboring components in the 30 S subunit.

    PubMed

    Wang, R; Alexander, R W; VanLoock, M; Vladimirov, S; Bukhtiyarov, Y; Harvey, S C; Cooperman, B S

    1999-02-19

    Nucleotides 518-533 form a loop in ribosomal 30 S subunits that is almost universally conserved. Both biochemical and genetic evidence clearly implicate the 530 loop in ribosomal function, with respect both to the accuracy control mechanism and to tRNA binding. Here, building on earlier work, we identify proteins and nucleotides (or limited sequences) site-specifically photolabeled by radioactive photolabile oligoDNA probes targeted toward the 530 loop of 30 S subunits. The probes we employ are complementary to 16 S rRNA nucleotides 517-527, and have aryl azides attached to nucleotides complementary to nucleotides 518, 522, and 525-527, positioning the photogenerated nitrene a maximum of 19-26 A from the complemented rRNA base. The crosslinks obtained are used as constraints to revise an earlier model of 30 S structure, using the YAMMP molecular modeling package, and to place the 530 loop region within that structure. Copyright 1999 Academic Press.

  13. Deducing the Kinetics of Protein Synthesis In Vivo from the Transition Rates Measured In Vitro

    PubMed Central

    Rudorf, Sophia; Thommen, Michael; Rodnina, Marina V.; Lipowsky, Reinhard

    2014-01-01

    The molecular machinery of life relies on complex multistep processes that involve numerous individual transitions, such as molecular association and dissociation steps, chemical reactions, and mechanical movements. The corresponding transition rates can be typically measured in vitro but not in vivo. Here, we develop a general method to deduce the in-vivo rates from their in-vitro values. The method has two basic components. First, we introduce the kinetic distance, a new concept by which we can quantitatively compare the kinetics of a multistep process in different environments. The kinetic distance depends logarithmically on the transition rates and can be interpreted in terms of the underlying free energy barriers. Second, we minimize the kinetic distance between the in-vitro and the in-vivo process, imposing the constraint that the deduced rates reproduce a known global property such as the overall in-vivo speed. In order to demonstrate the predictive power of our method, we apply it to protein synthesis by ribosomes, a key process of gene expression. We describe the latter process by a codon-specific Markov model with three reaction pathways, corresponding to the initial binding of cognate, near-cognate, and non-cognate tRNA, for which we determine all individual transition rates in vitro. We then predict the in-vivo rates by the constrained minimization procedure and validate these rates by three independent sets of in-vivo data, obtained for codon-dependent translation speeds, codon-specific translation dynamics, and missense error frequencies. In all cases, we find good agreement between theory and experiment without adjusting any fit parameter. The deduced in-vivo rates lead to smaller error frequencies than the known in-vitro rates, primarily by an improved initial selection of tRNA. The method introduced here is relatively simple from a computational point of view and can be applied to any biomolecular process, for which we have detailed information

  14. Behaviour of the Pleistocene marsupial lion deduced from claw marks in a southwestern Australian cave

    NASA Astrophysics Data System (ADS)

    Arman, Samuel D.; Prideaux, Gavin J.

    2016-02-01

    The marsupial lion, Thylacoleo carnifex, was the largest-ever marsupial carnivore, and is one of the most iconic extinct Australian vertebrates. With a highly-specialised dentition, powerful forelimbs and a robust build, its overall morphology is not approached by any other mammal. However, despite >150 years of attention, fundamental aspects of its biology remain unresolved. Here we analyse an assemblage of claw marks preserved on surfaces in a cave and deduce that they were generated by marsupial lions. The distribution and skewed size range of claw marks within the cave elucidate two key aspects of marsupial lion biology: they were excellent climbers and reared young in caves. Scrutiny of >10,000 co-located Pleistocene bones reveals few if any marsupial lion tooth marks, which dovetails with the morphology-based interpretation of the species as a flesh specialist.

  15. Behaviour of the Pleistocene marsupial lion deduced from claw marks in a southwestern Australian cave

    PubMed Central

    Arman, Samuel D.; Prideaux, Gavin J.

    2016-01-01

    The marsupial lion, Thylacoleo carnifex, was the largest-ever marsupial carnivore, and is one of the most iconic extinct Australian vertebrates. With a highly-specialised dentition, powerful forelimbs and a robust build, its overall morphology is not approached by any other mammal. However, despite >150 years of attention, fundamental aspects of its biology remain unresolved. Here we analyse an assemblage of claw marks preserved on surfaces in a cave and deduce that they were generated by marsupial lions. The distribution and skewed size range of claw marks within the cave elucidate two key aspects of marsupial lion biology: they were excellent climbers and reared young in caves. Scrutiny of >10,000 co-located Pleistocene bones reveals few if any marsupial lion tooth marks, which dovetails with the morphology-based interpretation of the species as a flesh specialist. PMID:26876952

  16. Oxygen plasma flow properties deduced from laser-induced fluorescence and probe measurements

    NASA Astrophysics Data System (ADS)

    Löhle, Stefan; Eichhorn, Christoph; Steinbeck, Andreas; Lein, Sebastian; Herdrich, Georg; Röser, Hans-Peter; Auweter-Kurtz, Monika

    2008-04-01

    Estimation of the local dissociation degree and the local mass-specific enthalpy of a pure oxygen plasma flow determined mainly from laser-induced fluorescence measurements are reported. Measurements have been conducted for several generator parameters in an inductively heated plasma wind tunnel. Additional probe measurements of total pressure together with the deduced translational temperature are used to estimate the local mass-specific enthalpy. For a reference condition, full dissociation has been measured. The measured translational temperature of atomic oxygen for this condition is T = 3500 K. Subsequently, the local mass-specific enthalpy has been derived using these local density and temperature measurements. For the reference condition the estimated value of h = 27 MJ/kg is in good agreement with the probe measurements and results from diode laser absorption spectroscopy.

  17. Temperature structure of active regions deduced from helium-like sulphur lines

    NASA Technical Reports Server (NTRS)

    Watanabe, Tetsuya; Hara, Hirohisa; Shimizu, Toshifumi; Hiei, Eijiro; Bentley, Robert D.; Lang, James; Phillips, Kenneth J. H.; Pike, C. David; Fludra, Andrzej; Bromage, Barbara J. I.

    1995-01-01

    Solar active-region temperatures have been determined from the full-Sun spectra of helium-like sulfur (S XV) observed by the Bragg Crystal Spectrometer on Board the Yohkoh satellite. The average temperature deduced from S XV is demonstrated to vary with the solar activity level: A temperature of 2.5 x 10(exp 6) K is derived from the spectra taken during low solar activity, similar to the general corona, while 4 x 10(exp 6) K is obtained during a higher activity phase. For the latter, the high- temperature tail of the differential emission measure of active regions is found most likely due to the superposition of numerous flare-like events (micro/nano-flares).

  18. Behaviour of the Pleistocene marsupial lion deduced from claw marks in a southwestern Australian cave.

    PubMed

    Arman, Samuel D; Prideaux, Gavin J

    2016-02-15

    The marsupial lion, Thylacoleo carnifex, was the largest-ever marsupial carnivore, and is one of the most iconic extinct Australian vertebrates. With a highly-specialised dentition, powerful forelimbs and a robust build, its overall morphology is not approached by any other mammal. However, despite >150 years of attention, fundamental aspects of its biology remain unresolved. Here we analyse an assemblage of claw marks preserved on surfaces in a cave and deduce that they were generated by marsupial lions. The distribution and skewed size range of claw marks within the cave elucidate two key aspects of marsupial lion biology: they were excellent climbers and reared young in caves. Scrutiny of >10,000 co-located Pleistocene bones reveals few if any marsupial lion tooth marks, which dovetails with the morphology-based interpretation of the species as a flesh specialist.

  19. Deducing fast electron density changes in randomly orientated uncrystallized biomolecules in a pump-probe experiment.

    PubMed

    Pande, K; Schwander, P; Schmidt, M; Saldin, D K

    2014-07-17

    We propose a method for deducing time-resolved structural changes in uncrystallized biomolecules in solution. The method relies on measuring the angular correlations of the intensities, when averaged over a large number of diffraction patterns from randomly oriented biomolecules in solution in a liquid solvent. The experiment is somewhat like a pump-probe version of an experiment on small angle X-ray scattering, except that the data expected by the algorithm are not just the radial variation of the averaged intensities. The differences of these correlation functions as measured from a photoexcited and dark structure enable the direct calculation of the difference electron density with a knowledge of only the dark structure. We exploit a linear relation we derive between the difference in these correlation functions and the difference electron density, applicable for small structural changes.

  20. Deducing fast electron density changes in randomly orientated uncrystallized biomolecules in a pump–probe experiment

    PubMed Central

    Pande, K.; Schwander, P.; Schmidt, M.; Saldin, D. K.

    2014-01-01

    We propose a method for deducing time-resolved structural changes in uncrystallized biomolecules in solution. The method relies on measuring the angular correlations of the intensities, when averaged over a large number of diffraction patterns from randomly oriented biomolecules in solution in a liquid solvent. The experiment is somewhat like a pump–probe version of an experiment on small angle X-ray scattering, except that the data expected by the algorithm are not just the radial variation of the averaged intensities. The differences of these correlation functions as measured from a photoexcited and dark structure enable the direct calculation of the difference electron density with a knowledge of only the dark structure. We exploit a linear relation we derive between the difference in these correlation functions and the difference electron density, applicable for small structural changes. PMID:24914159

  1. Martian low-altitude magnetic topology deduced from MAVEN/SWEA observations

    NASA Astrophysics Data System (ADS)

    Xu, Shaosui; Mitchell, David; Liemohn, Michael; Fang, Xiaohua; Ma, Yingjuan; Luhmann, Janet; Brain, David; Steckiewicz, Morgane; Mazelle, Christian; Connerney, Jack; Jacosky, Bruce

    2016-10-01

    The Mars Atmosphere and Volatile Evolution (MAVEN) mission for the first time make regular particle and field measurements down to ~150 km altitude. The Solar Wind Electron Analyzer (SWEA) instrument provides 3-D measurements of the electron energy and angular distributions. This study presents the pitch angle-resolved shape parameters that can separate photoelectrons from solar wind electrons, therefore used to deduce the Martian magnetic topology. The three-dimensional view of the magnetic topology is manifested for the first time. The northern hemisphere is found to be dominated by the crustal closed field lines, instead of draped interplanetary magnetic fields (IMF), on the dayside and more day-night connections through cross-terminator closed field lines than in the south. This study can also single out open field lines attached to the dayside ionosphere, which provide possible passage for ion outflow. Magnetic topology governs energetic electrons' movement, thus necessary to understand nightside ionosphere, and aurora.

  2. Characteristics of middle and upper tropospheric clouds as deduced from rawinsonde data

    NASA Technical Reports Server (NTRS)

    Starr, D. D. O.; Cox, S. K.

    1982-01-01

    The static environment of middle and upper tropospheric clouds is characterized. Computed relative humidity with respect to ice is used to diagnose the presence of cloud layer. The deduced seasonal mean cloud cover estimates based on this technique are shown to be reasonable. The cases are stratified by season and pressure thickness, and the dry static stability, vertical wind speed shear, and Richardson number are computed for three layers for each case. Mean values for each parameter are presented for each stratification and layer. The relative frequency of occurrence of various structures is presented for each stratification. The observed values of each parameter and the observed structure of each parameter are quite variable. Structures corresponding to any of a number of different conceptual models may be found. Moist adiabatic conditions are not commonly observed and the stratification based on thickness yields substantially different results for each group.

  3. Temperature structure of active regions deduced from helium-like sulphur lines

    NASA Technical Reports Server (NTRS)

    Watanabe, Tetsuya; Hara, Hirohisa; Shimizu, Toshifumi; Hiei, Eijiro; Bentley, Robert D.; Lang, James; Phillips, Kenneth J. H.; Pike, C. David; Fludra, Andrzej; Bromage, Barbara J. I.

    1995-01-01

    Solar active-region temperatures have been determined from the full-Sun spectra of helium-like sulfur (S XV) observed by the Bragg Crystal Spectrometer on Board the Yohkoh satellite. The average temperature deduced from S XV is demonstrated to vary with the solar activity level: A temperature of 2.5 x 10(exp 6) K is derived from the spectra taken during low solar activity, similar to the general corona, while 4 x 10(exp 6) K is obtained during a higher activity phase. For the latter, the high- temperature tail of the differential emission measure of active regions is found most likely due to the superposition of numerous flare-like events (micro/nano-flares).

  4. Wind speeds in two tornadic storms and a tornado, deduced from Doppler Spectra

    SciTech Connect

    Zrnic, D.; Istok, M.

    1980-12-01

    Doppler spectra of a tornado were collected with a radar having a large unambiguous velocity range, +- 91 m s/sup -1/. Thus for the first time a presentation of nonaliased spectra was possible, showing direct measurement of radial velocities. By fitting the tornado model spectrum to data, the radius of maximum winds and tornado center location are deduced. Tornado spectral signature is defined as a double peak, symmetric with respect to the mean wind spectrum. Histograms of maximum measured wind speeds (from spectrum skirts) for two tornadic storms are obtained, and the histograms of velocity difference (between the left and right spectrum skirt) suggest that smaller scale turbulence (<500 m) is principally responsible for spectrum broadness.

  5. An asymmetric color image encryption method by using deduced gyrator transform

    NASA Astrophysics Data System (ADS)

    Yao, Lili; Yuan, Caojin; Qiang, Junjie; Feng, Shaotong; Nie, Shouping

    2017-02-01

    An encryption algorithm is proposed by using the properties of deduced gyrator transform (GT). After being transformed by the GT algorithm and multiplied by a phase distribution p*, the spectrum modulus of the input image is considered to be the encrypted image by further performing Fourier transformation. To resist the attack from iterative phase retrieval, the red, green and blue components of the input image is modulated by a random phase mask and then combined using convolution. The encryption result is real-valued, which is convenient for display, transmission and storage. In the decryption process, the three original color components can be recovered with decryption keys which are different from the encryption keys. An optoelectronic hybrid system for the encryption process is also presented. Computer simulations are presented to demonstrate its performance, and the security of the proposed system is analyzed as well.

  6. Nucleotide and deduced amino acid sequences of a new subtilisin from an alkaliphilic Bacillus isolate.

    PubMed

    Saeki, Katsuhisa; Magallones, Marietta V; Takimura, Yasushi; Hatada, Yuji; Kobayashi, Tohru; Kawai, Shuji; Ito, Susumu

    2003-10-01

    The gene for a new subtilisin from the alkaliphilic Bacillus sp. KSM-LD1 was cloned and sequenced. The open reading frame of the gene encoded a 97 amino-acid prepro-peptide plus a 307 amino-acid mature enzyme that contained a possible catalytic triad of residues, Asp32, His66, and Ser224. The deduced amino acid sequence of the mature enzyme (LD1) showed approximately 65% identity to those of subtilisins SprC and SprD from alkaliphilic Bacillus sp. LG12. The amino acid sequence identities of LD1 to those of previously reported true subtilisins and high-alkaline proteases were below 60%. LD1 was characteristically stable during incubation with surfactants and chemical oxidants. Interestingly, an oxidizable Met residue is located next to the catalytic Ser224 of the enzyme as in the cases of the oxidation-susceptible subtilisins reported to date.

  7. Identification of thermophilic species by the amino acid compositions deduced from their genomes

    PubMed Central

    Kreil, David P.; Ouzounis, Christos A.

    2001-01-01

    The global amino acid compositions as deduced from the complete genomic sequences of six thermophilic archaea, two thermophilic bacteria, 17 mesophilic bacteria and two eukaryotic species were analysed by hierarchical clustering and principal components analysis. Both methods showed an influence of several factors on amino acid composition. Although GC content has a dominant effect, thermophilic species can be identified by their global amino acid compositions alone. This study presents a careful statistical analysis of factors that affect amino acid composition and also yielded specific features of the average amino acid composition of thermophilic species. Moreover, we introduce the first example of a ‘compositional tree’ of species that takes into account not only homologous proteins, but also proteins unique to particular species. We expect this simple yet novel approach to be a useful additional tool for the study of phylogeny at the genome level. PMID:11266564

  8. A major protein precursor of zebra mussel (Dreissena polymorpha) byssus: deduced sequence and significance.

    PubMed

    Anderson, K E; Waite, J H

    1998-04-01

    The zebra mussel is a nonindigenous invader of North American lakes and rivers and one of the few freshwater bivalve molluscs having a byssus--a sclerotized organ used by the mussel for opportunistic attachment to hard surfaces. We have sequenced a foot-specific cDNA whose composite protein sequence was deduced from a series of overlapping but occasionally nonidentical cDNA fragments. The overall deduced sequence matches tryptic peptides from a major byssal precursor protein--Dreissena polymorpha foot protein 1 (Dpfp1). The calculated mass of Dpfp1 is 49 kDa; but this is known to be extensively hydroxylated and O-glycosylated during maturation. Purified native Dpfp1 analyzed using matrix-assisted laser-desorption ionization mass spectrometry with time-of-flight indicates that the protein occurs as at least two size variants with masses of 48.6 and 54.5 kDa. In all probability, the sequence variants reported in this study are related to the larger mass variant. Dpfp1 has a block copolymer-like structure defined by two consensus motifs that are sharply segregated into domains. The N-terminal side of Dpfp1 has 22 tandem repeats of a heptapeptide consensus (P-[V/E]-Y-P-[T/S/delta]-[K/Q]-X); the C-terminal side has 16 repeats of a tridecapeptide motif (K-P-G-P-Y-D-Y-D-G-P-Y-D-K). Both consensus repeats are unique, with some limited homology to other proteins functioning in tension: marine mussel adhesives, plant extensins, titin, and trematode eggshell precursors.

  9. Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM and general features of the assembly process.

    PubMed

    Guo, Qiang; Goto, Simon; Chen, Yuling; Feng, Boya; Xu, Yanji; Muto, Akira; Himeno, Hyouta; Deng, Haiteng; Lei, Jianlin; Gao, Ning

    2013-02-01

    Ribosome biogenesis is a tightly regulated, multi-stepped process. The assembly of ribosomal subunits is a central step of the complex biogenesis process, involving nearly 30 protein factors in vivo in bacteria. Although the assembly process has been extensively studied in vitro for over 40 years, very limited information is known for the in vivo process and specific roles of assembly factors. Such an example is ribosome maturation factor M (RimM), a factor involved in the late-stage assembly of the 30S subunit. Here, we combined quantitative mass spectrometry and cryo-electron microscopy to characterize the in vivo 30S assembly intermediates isolated from mutant Escherichia coli strains with genes for assembly factors deleted. Our compositional and structural data show that the assembly of the 3'-domain of the 30S subunit is severely delayed in these intermediates, featured with highly underrepresented 3'-domain proteins and large conformational difference compared with the mature 30S subunit. Further analysis indicates that RimM functions not only to promote the assembly of a few 3'-domain proteins but also to stabilize the rRNA tertiary structure. More importantly, this study reveals intriguing similarities and dissimilarities between the in vitro and the in vivo assembly pathways, suggesting that they are in general similar but with subtle differences.

  10. Direct monitoring of initiation factor dynamics through formation of 30S and 70S translation-initiation complexes on a quartz crystal microbalance.

    PubMed

    Takahashi, Shuntaro; Isobe, Hidemi; Ueda, Takuya; Okahata, Yoshio

    2013-05-17

    Translation initiation is a dynamic and complicated process requiring the building a 70S initiation complex (70S-IC) composed of a ribosome, mRNA, and an initiator tRNA. During the formation of the 70S-IC, initiation factors (IFs: IF1, IF2, and IF3) interact with a ribosome to form a 30S initiation complex (30S-IC) and a 70S-IC. Although some spectroscopic analyses have been performed, the mechanism of binding and dissociation of IFs remains unclear. Here, we employed a 27 MHz quartz crystal microbalance (QCM) to evaluate the process of bacterial IC formation in translation initiation by following frequency changes (mass changes). IFs (IF1, IF2, and IF3), N-terminally fused to biotin carboxyl carrier protein (bio-BCCP), were immobilized on a Neutravidin-covered QCM plate. By using bio-BCCP-IF2 immobilized to the QCM, three steps of the formation of ribosomal initiation complex could be sequentially observed as simple mass changes in real time: binding of a 30S complex to the immobilized IF2, a recruitment of 50S to the 30S-IC, and formation of the 70S-IC. The kinetic parameters implied that the release of IF2 from the 70S-IC could be the rate-limiting step in translation initiation. The IF3-immobilized QCM revealed that the affinity of IF3 for the 30S complex decreased upon the addition of mRNA and fMet-tRNA(fMet) but did not lead to complete dissociation from the 30S-IC. These results suggest that IF3 binds and stays bound to ICs, and its interaction mode is altered during the formation of 30S-IC and 70S-IC and is finally induced to dissociate from ICs by 50S binding. This methodology demonstrated here is applicable to investigate the role of IFs in translation initiation driven by other pathways. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  11. The C-terminal helix in the YjeQ zinc-finger domain catalyzes the release of RbfA during 30S ribosome subunit assembly.

    PubMed

    Jeganathan, Ajitha; Razi, Aida; Thurlow, Brett; Ortega, Joaquin

    2015-06-01

    YjeQ (also called RsgA) and RbfA proteins in Escherichia coli bind to immature 30S ribosome subunits at late stages of assembly to assist folding of the decoding center. A key step for the subunit to enter the pool of actively translating ribosomes is the release of these factors. YjeQ promotes dissociation of RbfA during the final stages of maturation; however, the mechanism implementing this functional interplay has not been elucidated. YjeQ features an amino-terminal oligonucleotide/oligosaccharide binding domain, a central GTPase module and a carboxy-terminal zinc-finger domain. We found that the zinc-finger domain is comprised of two functional motifs: the region coordinating the zinc ion and a carboxy-terminal α-helix. The first motif is essential for the anchoring of YjeQ to the 30S subunit and the carboxy-terminal α-helix facilitates the removal of RbfA once the 30S subunit reaches the mature state. Furthermore, the ability of the mature 30S subunit to stimulate YjeQ GTPase activity also depends on the carboxy-terminal α-helix. Our data are consistent with a model in which YjeQ uses this carboxy-terminal α-helix as a sensor to gauge the conformation of helix 44, an essential motif of the decoding center. According to this model, the mature conformation of helix 44 is sensed by the carboxy-terminal α-helix, which in turn stimulates the YjeQ GTPase activity. Hydrolysis of GTP is believed to assist the release of YjeQ from the mature 30S subunit through a still uncharacterized mechanism. These results identify the structural determinants in YjeQ that implement the functional interplay with RbfA. © 2015 Jeganathan et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.

  12. Incidence of human astrovirus in central Australia (1995 to 1998) and comparison of deduced serotypes detected from 1981 to 1998.

    PubMed

    Schnagl, Roger D; Belfrage, Kate; Farrington, Rachel; Hutchinson, Kylie; Lewis, Victoria; Erlich, John; Morey, Fran

    2002-11-01

    The incidence of astrovirus infection was determined among infants and young children admitted to Alice Springs Hospital (central Australia) with gastroenteritis from 1995 to 1998. Astrovirus was detected by reverse transcription-PCR in 33 of 495 stool samples, and this represented 4.3% of a total of 774 stool samples tested for astrovirus, rotavirus, and Norwalk-like viruses. Astrovirus incidence was substantially lower than that of rotavirus but higher than that of Norwalk-like viruses both overall and in each of the 4 years individually. Over the period from 1981 to 1998, including the period from 1981 to 1994 during which astrovirus was identified only by electron microscopy, astrovirus serotypes (deduced from genotypes) 1, 2, 3, and 4 were identified. Deduced serotypes 1, 3, and 4 all appeared regularly over this 18-year period. Also over this period, nucleotide variation (in some cases substantial) in a section of the capsid protein precursor region of the virus genome was evident among strains of all four of the deduced central Australian serotypes. Consequent amino acid changes were, however, only evident among deduced serotype 3 strains. Geographic variation at both the genome and the resultant amino acid levels was evident among strains of all four of the deduced central Australian serotypes and their respective prototypes isolated in the United Kingdom.

  13. Deducing the 237U(n,f) cross-section using the Surrogate Ratio Method

    SciTech Connect

    Burke, J T; Bernstein, L A; Escher, J; Ahle, L; Church, J A; Dietrich, F; Moody, K J; Norman, E B; Phair, L W; Fallon, P; Clark, R; Delaplanque, M; Descovich, M; Cromaz, M; Lee, I Y; Macchiavelli, A O; McMahan, M A; Moretto, L G; Rodriguez-Vieitez, E; Stephens, F S

    2005-08-16

    The authors have deduced the {sup 237}U(n,f) cross-section over an equivalent neutron energy range of 0 to 20 MeV using the Surrogate Ratio method. A 55 MeV {sup 4}He{sup 2+} beam from the 88 Inch Cyclotron at Lawrence Berkeley National Laboratory was used to induce fission in the following reactions {sup 238}U({alpha},{alpha}'f) and {sup 236}U({alpha},{alpha}'f). The {sup 238}U reaction was a surrogate for {sup 237}U(n,f) and the {sup 236}U reaction was used as a surrogate for {sup 235}U(n,f). The energies of the scattered alpha particles were detected in a fully depleted segmented silicon telescope array (STARS) over an angle range of 35{sup o} to 60{sup o} with respect to the beam axis. The fission fragments were detected in a third independent silicon detector located at backward angles between 106{sup o} to 131{sup o}.

  14. Stratospheric aerosol acidity, density, and refractive index deduced from SAGE 2 and NMC temperature data

    NASA Technical Reports Server (NTRS)

    Yue, G. K.; Poole, L. R.; Wang, P.-H.; Chiou, E. W.

    1994-01-01

    Water vapor concentrations obtained by the Stratospheric Aerosol and Gas Experiment 2 (SAGE 2) and collocated temperatures provided by the National Meteorological Center (NMC) from 1986 to 1990 are used to deduce seasonally and zonally averaged acidity, density, and refractive index of stratospheric aerosols. It is found that the weight percentage of sulfuric acid in the aerosols increases from about 60 just above the tropopause to about 86 at 35 km. The density increases from about 1.55 to 1.85 g/cu cm between the same altitude limits. Some seasonal variations of composition and density are evident at high latitudes. The refractive indices at 1.02, 0.694, and 0.532 micrometers increase, respectively, from about 1.425, 1.430, and 1.435 just above the tropopause to about 1.445, 1.455, and 1.458 at altitudes above 27 km, depending on the season and latitude. The aerosol properties presented can be used in models to study the effectiveness of heterogeneous chemistry, the mass loading of stratospheric aerosols, and the extinction and backscatter of aerosols at different wavelengths. Computed aerosol surface areas, rate coefficients for the heterogeneous reaction ClONO2 + H2O yields HOCl + HNO3 and aerosol mass concentrations before and after the Pinatubo eruption in June 1991 are shown as sample applications.

  15. On the strength of the hydrogen-carbon interaction as deduced from physisorption.

    PubMed

    Nguyen, T X; Bae, J-S; Wang, Y; Bhatia, S K

    2009-04-21

    We deduce a new value for the potential well depth for the C-H2 interaction on the basis of experimental validations of isotherms of H2 and D2 predicted using independently characterized microstructural parameters. We use two carbons, one an activated carbon fiber whose structure has been recently characterized by us (Nguyen, T. X.; cohaut, N.; Bae, J.-S.; Bhatia, S. K. Langmuir 2008, 24, 7912) using hybrid reverse Monte Carlo simulation (HRMC) and the other the commercial Takeda 3A carbon molecular sieve whose pore size distribution is determined here from the 273 K CO2 adsorption isotherm. The conventional grand canonical Monte Carlo simulation technique incorporating a semiclassical Feynman and Hibbs (FH) potential approximation (FHGCMC) as well as path integral Monte Carlo calculations is employed to determine theoretical adsorption isotherms. It is found that curvature enhances the well depth for the LJ C-H2 interaction by a factor of 1.134 over that for a flat graphite surface, consistent with our recent study (Nguyen, T. X.; cohaut, N.; Bae, J.-S.; Bhatia, S. K. Langmuir 2008, 24, 7912). A value of the C-C well depth of 37.26 K, used for estimating the C-H2 well depth in conjunction with the Berthelot rules, with the Steele C-C well depth used for interaction with heavier gases (Ar, CO2 and CH4), leads to excellent agreement with experimental isotherms in all cases.

  16. Deducing the 237U(n,f) cross-section using the Surrogate Ratio Method

    SciTech Connect

    Burke, J T; Bernstein, L A; Escher, J; Ahle, L; Church, J A; Dietrich, F S; Moody, K J; Norman, E B; Phair, L; Fallon, P; Clark, R M; Deleplanque, M A; Descovich, M; Cromaz, M; Lee, I Y; Macchiavelli, A O; McMahan, M A; Moretto, L G; Rodriguez-Vieitez, E; Stephens, F S; Ai, H; Beausang, C; Cridder, B

    2005-12-29

    The authors have deduced the cross section for {sup 237}U(n,f) over an equivalent neutron energy range from 0 to 20 MeV using the Surrogate Ratio method. A 55 MeV {sup 4}He beam from the 88 Inch Cyclotron at Lawrence Berkeley National Laboratory was used to induce fission in the following reactions: {sup 238}U({alpha},{alpha}{prime}f) and {sup 236}U({alpha},{alpha}{prime}f). The {sup 238}U reaction was a surrogate for {sup 237}U(n,f) and the {sup 236}U reaction was used as a surrogate for {sup 235}U(n,f). Scattered alpha particles were detected in a fully depleted segmented silicon telescope array (STARS) over an angle range of 35{sup o} to 60{sup o} with respect to the beam axis. The fission fragments were detected in a third independent silicon detector located at backward angles between 106{sup o} and 131{sup o}.

  17. Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence

    SciTech Connect

    Millan, J.L.; Driscoll, C.E.; LeVan, K.M.; Goldberg, E.

    1987-08-01

    The sequence and structure of human testis-specific L-lactate dehydrogenase (LDHC/sub 4/, LDHX; (L)-lactate:NAD/sup +/ oxidoreductase, EC 1.1.1.27) has been derived from analysis of a complementary DNA (cDNA) clone comprising the complete protein coding region of the enzyme. From the deduced amino acid sequence, human LDHC/sub 4/ is as different from rodent LDHC/sub 4/ (73% homology) as it is from human LDHA/sub 4/ (76% homology) and porcine LDHB/sub 4/ (68% homology). Subunit homologies are consistent with the conclusion that the LDHC gene arose by at least two independent duplication events. Furthermore, the lower degree of homology between mouse and human LDHC/sub 4/ and the appearance of this isozyme late in evolution suggests a higher rate of mutation in the mammalian LDHC genes than in the LDHA and -B genes. Comparison of exposed amino acid residues of discrete anti-genic determinants of mouse and human LDHC/sub 4/ reveals significant differences. Knowledge of the human LDHC/sub 4/ sequence will help design human-specific peptides useful in the development of a contraceptive vaccine.

  18. Stratospheric aerosol acidity, density, and refractive index deduced from SAGE 2 and NMC temperature data

    NASA Technical Reports Server (NTRS)

    Yue, G. K.; Poole, L. R.; Wang, P.-H.; Chiou, E. W.

    1994-01-01

    Water vapor concentrations obtained by the Stratospheric Aerosol and Gas Experiment 2 (SAGE 2) and collocated temperatures provided by the National Meteorological Center (NMC) from 1986 to 1990 are used to deduce seasonally and zonally averaged acidity, density, and refractive index of stratospheric aerosols. It is found that the weight percentage of sulfuric acid in the aerosols increases from about 60 just above the tropopause to about 86 at 35 km. The density increases from about 1.55 to 1.85 g/cu cm between the same altitude limits. Some seasonal variations of composition and density are evident at high latitudes. The refractive indices at 1.02, 0.694, and 0.532 micrometers increase, respectively, from about 1.425, 1.430, and 1.435 just above the tropopause to about 1.445, 1.455, and 1.458 at altitudes above 27 km, depending on the season and latitude. The aerosol properties presented can be used in models to study the effectiveness of heterogeneous chemistry, the mass loading of stratospheric aerosols, and the extinction and backscatter of aerosols at different wavelengths. Computed aerosol surface areas, rate coefficients for the heterogeneous reaction ClONO2 + H2O yields HOCl + HNO3 and aerosol mass concentrations before and after the Pinatubo eruption in June 1991 are shown as sample applications.

  19. Relations between aliphatics and silicate components in 12 stratospheric particles deduced from vibrational spectroscopy

    SciTech Connect

    Merouane, S.; Djouadi, Z.; Le Sergeant d'Hendecourt, L.

    2014-01-10

    Interplanetary dust particles (IDPs) are among the most pristine extraterrestrial samples available in the laboratory for analyses with moderate to high spatial- and spectral-resolution spectroscopic techniques. Their composition can provide precious information on the early stages of the solar nebula as well as on the processes on the surfaces of different small bodies in the solar system from which IDPs originate. In this work, we have analyzed six anhydrous IDPs and six stratospheric particles possibly of cosmic origin through infrared (IR) and Raman micro-spectroscopy to study and investigate their silicate and organic components. We find that the length/ramification of the aliphatic organics given by the CH{sub 2}/CH{sub 3} ratios in the IDPs is closely linked to the silicate family (pyroxene or olivine) present in the samples. Both IR and Raman data suggest that this relation is not correlated with either aqueous (as evidenced by the absence of aqueous related minerals) or thermal processes (as deduced from Raman measurements). Therefore, this observation might be related to the initial path of formation of the organics on the silicate surfaces, thus tracing a possible catalytic role that silicates would play in the formation and/or ramification of organic matter in the primitive nebula.

  20. Drosophila melanogaster paramyosin: developmental pattern, mapping and properties deduced from its complete coding sequence.

    PubMed

    Vinós, J; Maroto, M; Garesse, R; Marco, R; Cervera, M

    1992-02-01

    Several cDNA clones encoding the complete Drosophila paramyosin sequence, including two potential polyadenylation sites, have been obtained. Southern analysis and in situ hybridization to polytene chromosomes indicate that in Drosophila the paramyosin gene is single copy, located on the left arm of the third chromosome at region 66D14. Northern analyses show predominantly two different RNAs which are the products of the choice between the two alternative polyadenylation sites. The two species begin to be synthesized around 10 h of development when embryonic muscles are formed, expression peaking at the end of embryogenesis. The protein is first expressed at germ band shortening in association with muscle precursor cells. A second maximum of paramyosin RNA expression occurs at late pupal stages when the higher molecular weight form becomes more abundant. In young adults this species becomes the main transcript detected. The 102 kDa polypeptide sequence is highly similar to that of Caenorhabditis elegans paramyosin. The protein has a central alpha-helical coiled-coil rod, organized in 29 groups of four typical seven-residue repeats and flanked by two short non-alpha-helical regions. Several leucine zippers are located on the hydrophobic face of the alpha-helix in paramyosin which, together with disulfide bonds between cysteines, are probably involved in the stabilization of the dimer. The structural and functional properties of Drosophila paramyosin deduced from the sequence are compared with those of known invertebrate myosins and paramyosins.

  1. Geoelectrical Structure of Ulleung Island, Korea Deduced from 3D MT Interpretations

    NASA Astrophysics Data System (ADS)

    Lee, T. J.; Kim, M. S.; Park, I. H.; Song, Y.

    2016-12-01

    A three-dimensional (3D) magnetotelluric (MT) survey has been carried out to delineate subsurface structures and possible fractures for geothermal development in Ulleung Island, Korea. Quite good quality of MT data could be obtained throughout the survey region by locating the remote reference in Sawauchi, Japan, which is approximately 900 km from the centre of the field site. 3D modelling and inversion are performed taking into account the sea effect in MT measurements near the seashore. The modelling results showed that seawater surrounding the Ulleung Island affected MT data at frequencies below 10 Hz, depending on the distance from the seashore. Two kinds of 3D inversion have been performed with the MT data observed from the area; ordinary 3D inversion, and inversion including the sea as a constraint. The sea-effect constraint inversion gave more reasonable and reliable results and showed clear E-W trend of conductive anomaly along the connection line between the test boreholes GH-1 and GH-2, which showed very high geothermal gradients of about 100 °/km. Comparing the gradients of the other two boreholes in north and south directions are of about 70 °/km, one can deduce that the deep circulation of convective water through the deeply connected fractures, say E-W trends conductive anomaly, carries the heat from the deep to near the surface. This can explain the big gradient differences from site to site in this small island.

  2. Deduced amino acid sequence of a putative sodium channel from the scyphozoan jellyfish Cyanea capillata.

    PubMed Central

    Anderson, P A; Holman, M A; Greenberg, R M

    1993-01-01

    Members of the phylum Cnidaria are the lowest extant organisms to possess a nervous system and are the first that are known to contain cells that produce action potentials carried exclusively by Na+ ions. They thus occupy an important position in the evolution of Na+ channels. A cDNA encoding a 198-kDa protein with high sequence identity to known Na+ channels was isolated from the scyphozoan jellyfish Cyanea capillata. The similarity between this and other Na+ channels is greatest in the transmembrane segments and the putative pore region and less so in the cytoplasmic loops that link the four domains of the protein. Phylogenetic analysis of the deduced protein reveals that it is closely related to known Na+ channels, particularly those of squid and Drosophila, and more distantly separated from Ca2+ channels. Scrutiny of the Cyanea channel in regions corresponding to those purported to form the tetrodotoxin receptor and selectivity filter of Na+ channels in higher animals reveals several anomalies that suggest that current models of the location of the tetrodotoxin binding site and Na+ channel selectivity filter are incomplete. Images Fig. 1 Fig. 2 Fig. 3 PMID:8394021

  3. Deduced amino acid sequence of a putative sodium channel from the scyphozoan jellyfish Cyanea capillata.

    PubMed

    Anderson, P A; Holman, M A; Greenberg, R M

    1993-08-01

    Members of the phylum Cnidaria are the lowest extant organisms to possess a nervous system and are the first that are known to contain cells that produce action potentials carried exclusively by Na+ ions. They thus occupy an important position in the evolution of Na+ channels. A cDNA encoding a 198-kDa protein with high sequence identity to known Na+ channels was isolated from the scyphozoan jellyfish Cyanea capillata. The similarity between this and other Na+ channels is greatest in the transmembrane segments and the putative pore region and less so in the cytoplasmic loops that link the four domains of the protein. Phylogenetic analysis of the deduced protein reveals that it is closely related to known Na+ channels, particularly those of squid and Drosophila, and more distantly separated from Ca2+ channels. Scrutiny of the Cyanea channel in regions corresponding to those purported to form the tetrodotoxin receptor and selectivity filter of Na+ channels in higher animals reveals several anomalies that suggest that current models of the location of the tetrodotoxin binding site and Na+ channel selectivity filter are incomplete.

  4. Bovine cone photoreceptor cGMP phosphodiesterase structure deduced from a cDNA clone.

    PubMed Central

    Li, T S; Volpp, K; Applebury, M L

    1990-01-01

    A full-length cDNA clone encoding the alpha' subunit of cGMP phosphodiesterase (PDE) from bovine cone photoreceptors was selected by probing a retinal library with a DNA fragment encoding the catalytic core of the rod cGMP PDE alpha subunit. Identity of the clone was confirmed by comparing its deduced sequence with cone PDE peptide sequences determined by Charbonneau et al. [Charbonneau, H., Prusti, R. K., LeTrong, H., Sonnenburg, W. K., Mullaney, P. J., Walsh, K. A. & Beavo, J. A. (1990) Proc. Natl. Acad. Sci. USA, pp. 288-292]. The cone PDE alpha' and the rod PDE alpha and beta subunits are encoded by distinct genes. cGMP PDE subunits share a common ancestry with cAMP PDEs and cyclic nucleotide-binding proteins. Sequence comparisons predict the presence of a catalytic core and possible secondary sites for noncatalytic cGMP binding. The presence of a C-terminal CAAX (Cys-aliphatic-aliphatic-Xaa) motif suggests the cone enzyme may be posttranslationally modified by proteolysis, methylation, and isoprenylation. Images PMID:2153291

  5. Interplanetary Magnetic Field Line Mixing Deduced from Impulsive Solar Flare Particles.

    PubMed

    Mazur; Mason; Dwyer; Giacalone; Jokipii; Stone

    2000-03-20

    We have studied fine-scale temporal variations in the arrival profiles of approximately 20 keV nucleon-1 to approximately 2 MeV nucleon-1 ions from impulsive solar flares using instrumentation on board the Advanced Composition Explorer spacecraft at 1 AU between 1997 November and 1999 July. The particle events often had short-timescale ( approximately 3 hr) variations in their intensity that occurred simultaneously across all energies and were generally not in coincidence with any local magnetic field or plasma signature. These features appear to be caused by the convection of magnetic flux tubes past the observer that are alternately filled and devoid of flare ions even though they had a common flare source at the Sun. Thus, we have used the particles to study the mixing of the interplanetary magnetic field that is due to random walk. We deduce an average timescale of 3.2 hr for these features, which corresponds to a length of approximately 0.03 AU.

  6. Function of longitudinal vs circular muscle fibers in esophageal peristalsis, deduced with mathematical modeling

    PubMed Central

    Brasseur, James G; Nicosia, Mark A; Pal, Anupam; Miller, Larry S

    2007-01-01

    We summarize from previous works the functions of circular vs. longitudinal muscle in esophageal peristaltic bolus transport using a mix of experimental data, the conservation laws of mechanics and mathematical modeling. Whereas circular muscle tone generates radial closure pressure to create a local peristaltic closure wave, longitudinal muscle tone has two functions, one physiological with mechanical implications, and one purely mechanical. Each of these functions independently reduces the tension of individual circular muscle fibers to maintain closure as a consequence of shortening of longitudinal muscle locally coordinated with increasing circular muscle tone. The physiological function is deduced by combining basic laws of mechanics with concurrent measurements of intraluminal pressure from manometry, and changes in cross sectional muscle area from endoluminal ultrasound from which local longitudinal shortening (LLS) can be accurately obtained. The purely mechanical function of LLS was discovered from mathematical modeling of peristaltic esophageal transport with the axial wall motion generated by LLS. Physiologically, LLS concentrates circular muscle fibers where closure pressure is highest. However, the mechanical function of LLS is to reduce the level of pressure required to maintain closure. The combined physiological and mechanical consequences of LLS are to reduce circular muscle fiber tension and power by as much as 1/10 what would be required for peristalsis without the longitudinal muscle layer, a tremendous benefit that may explain the existence of longitudinal muscle fiber in the gut. We also review what is understood of the role of longitudinal muscle in esophageal emptying, reflux and pathology. PMID:17457963

  7. Thin cirrus clouds - Seasonal distribution over oceans deduced from Nimbus-4 IRIS

    NASA Technical Reports Server (NTRS)

    Prabhakara, C.; Fraser, R. S.; Dalu, G.; Wu, Man-Li C.; Curran, R. J.

    1988-01-01

    Spectral differences in the extinction of the 10.8- and 12.6-micron bands of the IR window region, due to optically thin clouds, were found in the measurements made by both an airborne broadband IR radiometer and the IR interferometer spectrometer (IRIS) aboard the Nimbus-4 satellite; the extinction at 12.6 microns was significantly larger than that at 10.8 microns; both water and ice particles in the clouds can account for such spectral difference in extinction. Multiple scattering radiative transfer calculations of IRIS data revealed this spectral feature about 100 to 20 km away from the high-altitude cold clouds; it is assumed that this feature is related to the spreading of cirrus clouds. Based on this assumption, mean seasonal maps of the distribution of thin cirrus clouds over the oceans were deduced from the IRIS data. The maps show that such clouds are often present over the convectively active areas, such as ITCZ, SPCZ, and the Bay of Bengal during the summer monsoon.

  8. Atmospheric emissions and trends of nitrous oxide deduced from 10 years of ALE-GAGE data

    NASA Technical Reports Server (NTRS)

    Prinn, R.; Cunnold, D.; Alyea, F.; Rasmussen, R.; Simmonds, P.

    1990-01-01

    Long-term measurements of nitrous oxide (N2O) obtained during the Atmospheric Lifetime Experiment (ALE) and the Global Atmospheric Gases Experiment (GAGE) for a period from 1978 to 1988 are presented and interpreted. It is observed that the average concentration in the Northern Hemisphere is 0.75 +/- 0.16 ppbv higher than in the Southern Hemisphere and that the global average linear trend in N2O lies in the range from 0.25 to 0.31 percent/year. The measured trends and latitudinal distributions are shown to be consistent with the hypothesis that stratospheric photodissociation is the major atmospheric sink for N2O, while the cause of the N2O trend is suggested to be a combination of a growing tropical source and a growing Northern mid-latitude source. A 10-year average global N2O emission rate of (20.5 +/- 2.4) x 10 to the 12th g N2O/year is deduced from the ALE/GAGE data.

  9. Using Bayesian Evidence to Deduce the Dust-Attenuation Law at High Redshift

    NASA Astrophysics Data System (ADS)

    Salmon, Brett W.; Papovich, Casey J.; Finkelstein, Steven L.; Closson Ferguson, Henry; Long, James; CANDELS

    2016-01-01

    Although the nature of dust attenuation affects nearly all aspects of galaxy evolution, very little is known about the form of the dust-attenuation law in the distant Universe. Dust enshrouds and obscures UV star formation, convoluting our understanding of galaxy evolution at high redshift. Recent literature has recognized how the inferred physical properties of distant galaxies can be influenced by the non-universality of their attenuation curve shape. In this talk, I will present a Bayesian method to quantitatively constrain the dust-attenuation curve in high-redshift star-forming galaxies. This method is tested on galaxies at z~2 where we have CANDELS UV-to-optical photometry and Spitzer/Herschel IR luminosities. We find that the dust law implied from using only UV/optical data to calculate the full posterior probability densities supports the observed IR luminosities as predicted by that dust law. This method shows promise to deduce the shape of the attenuation curve at higher redshifts (z>4), as supported by our experiments using mock data from a semi-analytic model with qualities like those of the CANDELS GOODS fields.

  10. The Seismic risk perception in Italy deduced by a statistical sample

    NASA Astrophysics Data System (ADS)

    Crescimbene, Massimo; La Longa, Federica; Camassi, Romano; Pino, Nicola Alessandro; Pessina, Vera; Peruzza, Laura; Cerbara, Loredana; Crescimbene, Cristiana

    2015-04-01

    In 2014 EGU Assembly we presented the results of a web a survey on the perception of seismic risk in Italy. The data were derived from over 8,500 questionnaires coming from all Italian regions. Our questionnaire was built by using the semantic differential method (Osgood et al. 1957) with a seven points Likert scale. The questionnaire is inspired the main theoretical approaches of risk perception (psychometric paradigm, cultural theory, etc.) .The results were promising and seem to clearly indicate an underestimation of seismic risk by the italian population. Based on these promising results, the DPC has funded our research for the second year. In 2015 EGU Assembly we present the results of a new survey deduced by an italian statistical sample. The importance of statistical significance at national scale was also suggested by ISTAT (Italian Statistic Institute), considering the study as of national interest, accepted the "project on the perception of seismic risk" as a pilot study inside the National Statistical System (SISTAN), encouraging our RU to proceed in this direction. The survey was conducted by a company specialised in population surveys using the CATI method (computer assisted telephone interview). Preliminary results will be discussed. The statistical support was provided by the research partner CNR-IRPPS. This research is funded by Italian Civil Protection Department (DPC).

  11. Joint trajectories of cigarette smoking and depressive symptoms from the mid-20s to the mid-30s predicting generalized anxiety disorder.

    PubMed

    Lee, Jung Yeon; Brook, Judith S; Finch, Stephen J; De La Rosa, Mario; Brook, David W

    2017-03-10

    The current study examines longitudinal patterns of cigarette smoking and depressive symptoms as predictors of generalized anxiety disorder using data from the Harlem Longitudinal Development Study. There were 674 African American (53%) and Puerto Rican (47%) participants. Among the 674 participants, 60% were females. In the logistic regression analyses, the indicators of membership in each of the joint trajectories of cigarette smoking and depressive symptoms from the mid-20s to the mid-30s were used as the independent variables, and the diagnosis of generalized anxiety disorder in the mid-30s was used as the dependent variable. The high cigarette smoking with high depressive symptoms group and the low cigarette smoking with high depressive symptoms group were associated with an increased likelihood of having generalized anxiety disorder as compared to the no cigarette smoking with low depressive symptoms group. The findings shed light on the prevention and treatment of generalized anxiety disorder.

  12. Characteristics of Turbulent Airflow Deduced from Rapid Surface Thermal Fluctuations: An Infrared Surface Anemometer

    NASA Astrophysics Data System (ADS)

    Aminzadeh, Milad; Breitenstein, Daniel; Or, Dani

    2017-07-01

    The intermittent nature of turbulent airflow interacting with the surface is readily observable in fluctuations of the surface temperature resulting from the thermal imprints of eddies sweeping the surface. Rapid infrared thermography has recently been used to quantify characteristics of the near-surface turbulent airflow interacting with the evaporating surfaces. We aim to extend this technique by using single-point rapid infrared measurements to quantify properties of a turbulent flow, including surface exchange processes, with a view towards the development of an infrared surface anemometer. The parameters for the surface-eddy renewal (α and β ) are inferred from infrared measurements of a single-point on the surface of a heat plate placed in a wind tunnel with prescribed wind speeds and constant mean temperatures of the surface. Thermally-deduced parameters are in agreement with values obtained from standard three-dimensional ultrasonic anemometer measurements close to the plate surface (e.g., α = 3 and β = 1/26 (ms)^{-1} for the infrared, and α = 3 and β = 1/19 (ms)^{-1} for the sonic-anemometer measurements). The infrared-based turbulence parameters provide new insights into the role of surface temperature and buoyancy on the inherent characteristics of interacting eddies. The link between the eddy-spectrum shape parameter α and the infrared window size representing the infrared field of view is investigated. The results resemble the effect of the sampling height above the ground in sonic anemometer measurements, which enables the detection of larger eddies with higher values of α . The physical basis and tests of the proposed method support the potential for remote quantification of the near-surface momentum field, as well as scalar-flux measurements in the immediate vicinity of the surface.

  13. Deducing Electronic Unit Internal Response During a Vibration Test Using a Lumped Parameter Modeling Approach

    NASA Technical Reports Server (NTRS)

    Van Dyke, Michael B.

    2014-01-01

    During random vibration testing of electronic boxes there is often a desire to know the dynamic response of certain internal printed wiring boards (PWBs) for the purpose of monitoring the response of sensitive hardware or for post-test forensic analysis in support of anomaly investigation. Due to restrictions on internally mounted accelerometers for most flight hardware there is usually no means to empirically observe the internal dynamics of the unit, so one must resort to crude and highly uncertain approximations. One common practice is to apply Miles Equation, which does not account for the coupled response of the board in the chassis, resulting in significant over- or under-prediction. This paper explores the application of simple multiple-degree-of-freedom lumped parameter modeling to predict the coupled random vibration response of the PWBs in their fundamental modes of vibration. A simple tool using this approach could be used during or following a random vibration test to interpret vibration test data from a single external chassis measurement to deduce internal board dynamics by means of a rapid correlation analysis. Such a tool might also be useful in early design stages as a supplemental analysis to a more detailed finite element analysis to quickly prototype and analyze the dynamics of various design iterations. After developing the theoretical basis, a lumped parameter modeling approach is applied to an electronic unit for which both external and internal test vibration response measurements are available for direct comparison. Reasonable correlation of the results demonstrates the potential viability of such an approach. Further development of the preliminary approach presented in this paper will involve correlation with detailed finite element models and additional relevant test data.

  14. Nucleotide and deduced amino acid sequences of rat myosin binding protein H (MyBP-H).

    PubMed

    Jung, J; Oh, J; Lee, K

    1998-12-01

    The complete nucleotide sequence of the cDNA clone encoding rat skeletal muscle myosin-binding protein H (MyBP-H) was determined and amino acid sequence was deduced from the nucleotide sequence (GenBank accession number AF077338). The full-length cDNA of 1782 base pairs(bp) contains a single open reading frame of 1454 bp encoding a rat MyBP-H protein of the predicted molecular mass 52.7 kDa and includes the common consensus 'CA__TG' protein binding motif. The cDNA sequence of rat MyBP-H show 92%, 84% and 41% homology with those of mouse, human and chicken, respectively. The protein contains tandem internal motifs array (-FN III-Ig C2-FN III-Ig C2-) in the C-terminal region which resembles to the immunoglobulin superfamily C2 and fibronectin type III motifs. The amino acid sequence of the C-terminal Ig C2 was highly conserved among MyBPs family and other thick filament binding proteins, suggesting that the C-terminal Ig C2 might play an important role in its function. All proteins belonging to MyBP-H member contains 'RKPS' sequence which is assumed to be cAMP- and cGMP-dependent protein kinase A phosphorylation site. Computer analysis of the primary sequence of rat MyBP-H predicted 11 protein kinase C (PKC) phosphorylation site, 7 casein kinase II (CK2) phosphorylation site and 4 N-myristoylation site.

  15. Characteristics of tropical thin cirrus clouds deduced from joint CloudSat and CALIPSO observations

    NASA Astrophysics Data System (ADS)

    Haladay, Taryn; Stephens, Graeme

    2009-04-01

    The joint detection characteristics of both the CloudSat radar and Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observations (CALIPSO) lidar are used to study tropical thin cirrus observed between 20°N and 20°S. The thin ice cloud category (TIC-1) of cirrus consists of those clouds detected by the lidar but not the radar whereas the TIC-2 cirrus category consists of clouds detected by both sensors. Tropical TIC-1 cirrus clouds between 20°N and 20°S are high, are optically thin, and have an approximate cloud cover in the defined region of 30%. Almost a third of this occurrence is in the form of single layers of cloudiness without any clouds below. These TIC-1 clouds also exhibit a marked seasonal variation, especially away from the equator, consistent with the shifts in annual cycle of convection with latitude. Lidar-based estimates of optical depth, uncorrected for multiple scattering, suggest that the TIC-1 optical depths range between 0.02 and 0.3. The ice water path of TIC-1 clouds is also estimated to be between 0.5 and 4 g m-2. The radiative properties of the TIC-1 clouds are also deduced from CloudSat flux data products at the top, at the bottom, and within the atmosphere. The influence of these clouds on the instantaneous reflected solar fluxes is determined to be less than 2 W m-2. The effects of TIC-1 clouds on the instantaneous outgoing longwave fluxes are estimated to be ˜20 W m-2, and the impact of these TIC-1 clouds on the tropics-wide average of the infrared heating is ˜4 W m-2.

  16. Deducing Electronic Unit Internal Response During a Vibration Test Using a Lumped Parameter Modeling Approach

    NASA Technical Reports Server (NTRS)

    Van Dyke, Michael B.

    2014-01-01

    During random vibration testing of electronic boxes there is often a desire to know the dynamic response of certain internal printed wiring boards (PWBs) for the purpose of monitoring the response of sensitive hardware or for post-test forensic analysis in support of anomaly investigation. Due to restrictions on internally mounted accelerometers for most flight hardware there is usually no means to empirically observe the internal dynamics of the unit, so one must resort to crude and highly uncertain approximations. One common practice is to apply Miles Equation, which does not account for the coupled response of the board in the chassis, resulting in significant over- or under-prediction. This paper explores the application of simple multiple-degree-of-freedom lumped parameter modeling to predict the coupled random vibration response of the PWBs in their fundamental modes of vibration. A simple tool using this approach could be used during or following a random vibration test to interpret vibration test data from a single external chassis measurement to deduce internal board dynamics by means of a rapid correlation analysis. Such a tool might also be useful in early design stages as a supplemental analysis to a more detailed finite element analysis to quickly prototype and analyze the dynamics of various design iterations. After developing the theoretical basis, a lumped parameter modeling approach is applied to an electronic unit for which both external and internal test vibration response measurements are available for direct comparison. Reasonable correlation of the results demonstrates the potential viability of such an approach. Further development of the preliminary approach presented in this paper will involve correlation with detailed finite element models and additional relevant test data.

  17. A novel computational framework for deducing muscle synergies from experimental joint moments

    PubMed Central

    Gopalakrishnan, Anantharaman; Modenese, Luca; Phillips, Andrew T. M.

    2014-01-01

    Prior experimental studies have hypothesized the existence of a “muscle synergy” based control scheme for producing limb movements and locomotion in vertebrates. Such synergies have been suggested to consist of fixed muscle grouping schemes with the co-activation of all muscles in a synergy resulting in limb movement. Quantitative representations of these groupings (termed muscle weightings) and their control signals (termed synergy controls) have traditionally been derived by the factorization of experimentally measured EMG. This study presents a novel approach for deducing these weightings and controls from inverse dynamic joint moments that are computed from an alternative set of experimental measurements—movement kinematics and kinetics. This technique was applied to joint moments for healthy human walking at 0.7 and 1.7 m/s, and two sets of “simulated” synergies were computed based on two different criteria (1) synergies were required to minimize errors between experimental and simulated joint moments in a musculoskeletal model (pure-synergy solution) (2) along with minimizing joint moment errors, synergies also minimized muscle activation levels (optimal-synergy solution). On comparing the two solutions, it was observed that the introduction of optimality requirements (optimal-synergy) to a control strategy solely aimed at reproducing the joint moments (pure-synergy) did not necessitate major changes in the muscle grouping within synergies or the temporal profiles of synergy control signals. Synergies from both the simulated solutions exhibited many similarities to EMG derived synergies from a previously published study, thus implying that the analysis of the two different types of experimental data reveals similar, underlying synergy structures. PMID:25520645

  18. Structure of La Primavera caldera, Jalisco, Mexico, deduced from gravity anomalies and drilling results

    NASA Astrophysics Data System (ADS)

    Yokoyama, I.; Mena, M.

    1991-07-01

    Previous studies of La Primavera caldera have mostly been based on surface geology and topography. Since 1980, many wells, exploring for geothermal energy, have reached depths of about 2 to 3 km at the center of the caldera. The results of the drillings, together with those of the gravity surveys, provide information about the subsurface structure of the caldera, and shed light on its formation. The drilling results and gravity anomalies at La Primavera caldera and San Marcos, located at about 40 km distance from the caldera, suggest that regional gravity anomalies can be interpreted in terms of depths of the granitic basements: the basement beneath La Primavera caldera is about 3 km deep and consists of roughly the same horizon as that beneath San Marcos. The drilling results within the caldera reveal that the depth of the caldera fills ranges from 0.3 to 1 km at the drilling sites. The andesite basement, about 1 km deep, remains approximately horizontal, and the granitic basement has a depth of about 3 km. The surface topographies, such as the postcaldera domes, scarcely disturb the subsurface strata. The local gravity anomalies show two lows within the caldera reflecting the configuration of caldera bottom, two funnel-shaped depressions, one of which corresponds to a vent of the Tala tuff deduced from geological observations. The mass deficiency within the caldera estimated from the gravity anomaly, satisfies the general relationship that the mass deficiency is proportional to the caldera diameter cubed. This means that caldera structure is three-dimensional: the larger the diameter, the deeper the funnel-shape. At present this argument may be limited to funnel-shaped calderas.

  19. The radiation budget of a Cirrus layer deduced from simultaneous aircraft observations and model calculations

    NASA Technical Reports Server (NTRS)

    Ackerman, Thomas P.; Kinne, Stefan A.; Heymsfield, Andrew J.; Valero, Francisco P. J.

    1990-01-01

    Several aircraft were employed during the FIRE Cirrus IFO in order to make nearly simultaneous observations of cloud properties and fluxes. A segment of the flight data collected on 28 October 1988 during which the NASA Ames ER-2 overflew the NCAR King Air was analyzed. The ER-2 flew at high altitude making observations of visible and infrared radiances and infrared flux and cloud height and thickness. During this segment, the King Air flew just above the cloud base making observations of ice crystal size and shape, local meteorological variables, and infrared fluxes. While the two aircraft did not collect data exactly coincident in space and time, they did make observations within a few minutes of each other. For this case study, the infrared radiation balance of the cirrus layer is of primary concern. Observations of the upwelling 10 micron radiance, made from the ER-2, can be used to deduce the 10 micron optical depth of the layer. The upwelling broadband infrared flux is also measured from the ER-2. At the same time, the upwelling and downwelling infrared flux at the cloud base is obtained from the King Air measurements. Information on cloud microphysics is also available from the King Air. Using this data in conjunction with atmospheric temperature and humidity profiles from local radiosondes, the necessary inputs for an infrared radiative transfer model can be developed. Infrared radiative transfer calculations are performed with a multispectral two-stream model. The model fluxes at the cloud base and at 19 km are then compared with the aircraft observations to determine whether the model is performing well. Cloud layer heating rates can then be computed from the radiation exchange.

  20. Martian low-altitude magnetic topology deduced from MAVEN/SWEA observations

    NASA Astrophysics Data System (ADS)

    Xu, Shaosui; Mitchell, David; Liemohn, Michael; Fang, Xiaohua; Ma, Yingjuan; Luhmann, Janet; Brain, David; Steckiewicz, Morgane; Mazelle, Christian; Connerney, Jack; Jakosky, Bruce

    2017-02-01

    The Mars Atmosphere and Volatile Evolution mission has obtained comprehensive particle and magnetic field measurements. The Solar Wind Electron Analyzer provides electron energy-pitch angle distributions along the spacecraft trajectory that can be used to infer magnetic topology. This study presents pitch angle-resolved electron energy shape parameters that can distinguish photoelectrons from solar wind electrons, which we use to deduce the Martian magnetic topology and connectivity to the dayside ionosphere. Magnetic topology in the Mars environment is mapped in three dimensions for the first time. At low altitudes (<400 km) in sunlight, the northern hemisphere is found to be dominated by closed field lines (both ends intersecting the collisional atmosphere), with more day-night connections through cross-terminator closed field lines than in the south. Although draped field lines with 100 km amplitude vertical fluctuations that intersect the electron exobase ( 160-220 km) in two locations could appear to be closed at the spacecraft, a more likely explanation is provided by crustal magnetic fields, which naturally have the required geometry. Around 30% of the time, we observe open field lines from 200 to 400 km, which implies three distinct topological layers over the northern hemisphere: closed field lines below 200 km, open field lines with foot points at lower latitudes that pass over the northern hemisphere from 200 to 400 km, and draped interplanetary magnetic field above 400 km. This study also identifies open field lines with one end attached to the dayside ionosphere and the other end connected with the solar wind, providing a path for ion outflow.

  1. Deducing logical relationships between spatially registered cortical parcellations under conditions of uncertainty.

    PubMed

    Bezgin, Gleb; Wanke, Egon; Krumnack, Antje; Kötter, Rolf

    2008-10-01

    We propose a new technique, called Spatial Objective Relational Transformation (SORT), as an automated approach for derivation of logical relationships between cortical areas in different brain maps registered in the same Euclidean space. Recently, there have been large amounts of voxel-based three-dimensional structural and functional imaging data that provide us with coordinate-based information about the location of differently defined areas in the brain, whereas coordinate-independent, parcellation-based mapping is still commonly used in the majority of animal tracing and mapping studies. Because of the impact of voxel-based imaging methods and the need to attribute their features to coordinate-independent brain entities, this mapping becomes increasingly important. Our motivation here is not to make vague statements where more precise spatial statements would be better, but to find criteria for the identity (or other logical relationships) between areas that were delineated by different methods, in different individuals, or mapped to three-dimensional space using different deformation algorithms. The relevance of this problem becomes immediately obvious as one superimposes and compares different datasets in multimodal databases (e.g. CARET, http://brainmap.wustl.edu/caret), where voxel-based data are registered to surface nodes exploited by the procedure presented here. We describe the SORT algorithm and its implementation in the Java 2 programming language (http://java.sun.com/, which we make available for download. We give an example of practical use of our approach, and validate the SORT approach against a database of the coordinate-independent statements and inferences that have been deduced using alternative techniques.

  2. Deducing growth mechanisms for minerals from the shapes of crystal size distributions

    USGS Publications Warehouse

    Eberl, D.D.; Drits, V.A.; Srodon, J.

    1998-01-01

    Crystal size distributions (CSDs) of natural and synthetic samples are observed to have several distinct and different shapes. We have simulated these CSDs using three simple equations: the Law of Proportionate Effect (LPE), a mass balance equation, and equations for Ostwald ripening. The following crystal growth mechanisms are simulated using these equations and their modifications: (1) continuous nucleation and growth in an open system, during which crystals nucleate at either a constant, decaying, or accelerating nucleation rate, and then grow according to the LPE; (2) surface-controlled growth in an open system, during which crystals grow with an essentially unlimited supply of nutrients according to the LPE; (3) supply-controlled growth in an open system, during which crystals grow with a specified, limited supply of nutrients according to the LPE; (4) supply- or surface-controlled Ostwald ripening in a closed system, during which the relative rate of crystal dissolution and growth is controlled by differences in specific surface area and by diffusion rate; and (5) supply-controlled random ripening in a closed system, during which the rate of crystal dissolution and growth is random with respect to specific surface area. Each of these mechanisms affects the shapes of CSDs. For example, mechanism (1) above with a constant nucleation rate yields asymptotically-shaped CSDs for which the variance of the natural logarithms of the crystal sizes (??2) increases exponentially with the mean of the natural logarithms of the sizes (??). Mechanism (2) yields lognormally-shaped CSDs, for which ??2 increases linearly with ??, whereas mechanisms (3) and (5) do not change the shapes of CSDs, with ??2 remaining constant with increasing ??. During supply-controlled Ostwald ripening (4), initial lognormally-shaped CSDs become more symmetric, with ??2 decreasing with increasing ??. Thus, crystal growth mechanisms often can be deduced by noting trends in ?? versus ??2 of CSDs for

  3. Early Success in Using the Relation between Stimuli and Rewards To Deduce an Abstract Rule: Perceived Physical Connection Is Key.

    ERIC Educational Resources Information Center

    Diamond, Adele; Lee, EunYoung; Hayden, Michael

    2003-01-01

    Two studies examined 9- to 15-month-olds' ability to deduce an abstract nonmatching rule from reward feedback. Results showed that physical connectedness between stimuli and reward was key to performance. In the absence of the perception that stimulus and reward were components of a single thing, even close spatial and temporal proximity were…

  4. Connections between SST and cyclonicity parameters as deduced from empirical data and GCM runs with prescribed SST

    SciTech Connect

    Bardin, M.Yu.; Rubinstein, K.G.

    1997-12-31

    Previously the authors investigated separately effects of the underlying surface on climate variables (among them, parameters of cyclonicity) by empirical model data. The present study has a twofold goal: (i) to investigate model capabilities in reproducing relationships between SST and cyclonicity and (ii) deduce possible causes of certain large-scale climate anomalies, reflected in cyclone parameters, from model experiment data.

  5. Comparison of transcripts in Phalaenopsis bellina and Phalaenopsis equestris (Orchidaceae) flowers to deduce monoterpene biosynthesis pathway

    PubMed Central

    Hsiao, Yu-Yun; Tsai, Wen-Chieh; Kuoh, Chang-Sheng; Huang, Tian-Hsiang; Wang, Hei-Chia; Wu, Tian-Shung; Leu, Yann-Lii; Chen, Wen-Huei; Chen, Hong-Hwa

    2006-01-01

    Background Floral scent is one of the important strategies for ensuring fertilization and for determining seed or fruit set. Research on plant scents has hampered mainly by the invisibility of this character, its dynamic nature, and complex mixtures of components that are present in very small quantities. Most progress in scent research, as in other areas of plant biology, has come from the use of molecular and biochemical techniques. Although volatile components have been identified in several orchid species, the biosynthetic pathways of orchid flower fragrance are far from understood. We investigated how flower fragrance was generated in certain Phalaenopsis orchids by determining the chemical components of the floral scent, identifying floral expressed-sequence-tags (ESTs), and deducing the pathways of floral scent biosynthesis in Phalaneopsis bellina by bioinformatics analysis. Results The main chemical components in the P. bellina flower were shown by gas chromatography-mass spectrometry to be monoterpenoids, benzenoids and phenylpropanoids. The set of floral scent producing enzymes in the biosynthetic pathway from glyceraldehyde-3-phosphate (G3P) to geraniol and linalool were recognized through data mining of the P. bellina floral EST database (dbEST). Transcripts preferentially expressed in P. bellina were distinguished by comparing the scent floral dbEST to that of a scentless species, P. equestris, and included those encoding lipoxygenase, epimerase, diacylglycerol kinase and geranyl diphosphate synthase. In addition, EST filtering results showed that transcripts encoding signal transduction and Myb transcription factors and methyltransferase, in addition to those for scent biosynthesis, were detected by in silico hybridization of the P. bellina unigene database against those of the scentless species, rice and Arabidopsis. Altogether, we pinpointed 66% of the biosynthetic steps from G3P to geraniol, linalool and their derivatives. Conclusion This systems

  6. Traveling Wave Packets of Total Electron Content Disturbances As Deduced From Global GPS Network Data

    NASA Astrophysics Data System (ADS)

    Afraimovich, E.; Lesyuta, O.; Lipko, Yu.; Perevalova, N.; Voyeikov, S.; Vodyannikov, V.; Yakovets, A.; Jacobi, Ch.

    This report discusses the experimental research results on the morphology and physi- cal origin of total electron content (TEC) pulsations as measured using the data from the global GPS network. Periodic electron density oscillations of the type of wave packets were investigated previously in terms of the hypothesis of their association with geomagnetic field (GP) pulsations. The greater part of evidence of the association between GP ad periodic electron density oscillations in the ionosphere was obtained by recording the frequency Doppler shift if the ionosphere-reflected radio signal and TEC variations measured using signals from geostationary satellites. However, many years of investigations have not yet provided thorough insight into the mechanisms ac- counting for the linkage between GP and ionospheric variations. One reason for that is the difficulty associated with obtaining statistically significant sets of experimental data. The use of the international ground-based network of two-frequency receivers of the navigation GPS system which comprised no less than 900 site as of August 2001 and is currently placing the data on the Internet, opens up a new era of a global, con- tinuous and fully computerized monitoring of ionospheric disturbances of a different class. This report presents a global morphology of TEC pulsations for 50 days with a different level of geomagnetic activity and the number of stations of the global GPS network from 100 to 300. A total number of the "receiver - GPS satellites" radio paths used in the analysis is about 500,000. Quasi-periodic TEC variations in the range of periods from 10 to 20 min are investigated, which is dictated by the fact that the data from the global GPS network are placed on the Internet with a standard temporal res- olution of 30 s. Most often, the observed TEC pulsations represent wave packets with a duration on the order of 1 hour. It was found that such TEC pulsations are a rela- tively rare event and are

  7. An evaluation of the 30-s chair stand test in older adults: frailty detection based on kinematic parameters from a single inertial unit

    PubMed Central

    2013-01-01

    Background A growing interest in frailty syndrome exists because it is regarded as a major predictor of co-morbidities and mortality in older populations. Nevertheless, frailty assessment has been controversial, particularly when identifying this syndrome in a community setting. Performance tests such as the 30-second chair stand test (30-s CST) are a cornerstone for detecting early declines in functional independence. Additionally, recent advances in body-fixed sensors have enhanced the sensors’ ability to automatically and accurately evaluate kinematic parameters related to a specific movement performance. The purpose of this study is to use this new technology to obtain kinematic parameters that can identify frailty in an aged population through the performance the 30-s CST. Methods Eighteen adults with a mean age of 54 years, as well as sixteen pre-frail and thirteen frail patients with mean ages of 78 and 85 years, respectively, performed the 30-s CST while threir trunk movements were measured by a sensor-unit at vertebra L3. Sit-stand-sit cycles were determined using both acceleration and orientation information to detect failed attempts. Movement-related phases (i.e. impulse, stand-up, and sit-down) were differentiated based on seat off and seat on events. Finally, the kinematic parameters of the impulse, stand-up and sit-down phases were obtained to identify potential differences across the three frailty groups. Results For the stand-up and sit-down phases, velocity peaks and “modified impulse” parameters clearly differentiated subjects with different frailty levels (p < 0.001). The trunk orientation range during the impulse phase was also able to classify a subject according to his frail syndrome (p < 0.001). Furthermore, these parameters derived from the inertial units (IUs) are sensitive enough to detect frailty differences not registered by the number of completed cycles which is the standard test outcome. Conclusions This study shows

  8. An evaluation of the 30-s chair stand test in older adults: frailty detection based on kinematic parameters from a single inertial unit.

    PubMed

    Millor, Nora; Lecumberri, Pablo; Gómez, Marisol; Martínez-Ramírez, Alicia; Izquierdo, Mikel

    2013-08-01

    A growing interest in frailty syndrome exists because it is regarded as a major predictor of co-morbidities and mortality in older populations. Nevertheless, frailty assessment has been controversial, particularly when identifying this syndrome in a community setting. Performance tests such as the 30-second chair stand test (30-s CST) are a cornerstone for detecting early declines in functional independence. Additionally, recent advances in body-fixed sensors have enhanced the sensors' ability to automatically and accurately evaluate kinematic parameters related to a specific movement performance. The purpose of this study is to use this new technology to obtain kinematic parameters that can identify frailty in an aged population through the performance the 30-s CST. Eighteen adults with a mean age of 54 years, as well as sixteen pre-frail and thirteen frail patients with mean ages of 78 and 85 years, respectively, performed the 30-s CST while their trunk movements were measured by a sensor-unit at vertebra L3. Sit-stand-sit cycles were determined using both acceleration and orientation information to detect failed attempts. Movement-related phases (i.e. impulse, stand-up, and sit-down) were differentiated based on seat off and seat on events. Finally, the kinematic parameters of the impulse, stand-up and sit-down phases were obtained to identify potential differences across the three frailty groups. For the stand-up and sit-down phases, velocity peaks and "modified impulse" parameters clearly differentiated subjects with different frailty levels (p < 0.001). The trunk orientation range during the impulse phase was also able to classify a subject according to his frail syndrome (p < 0.001). Furthermore, these parameters derived from the inertial units (IUs) are sensitive enough to detect frailty differences not registered by the number of completed cycles which is the standard test outcome. This study shows that IUs can enhance the information gained from

  9. DEDUCE Clinical Text: An Ontology-based Module to Support Self-Service Clinical Notes Exploration and Cohort Development.

    PubMed

    Roth, Christopher; Rusincovitch, Shelley A; Horvath, Monica M; Brinson, Stephanie; Evans, Steve; Shang, Howard C; Ferranti, Jeffrey M

    2013-01-01

    Large amounts of information, as well as opportunities for informing research, education, and operations, are contained within clinical text such as radiology reports and pathology reports. However, this content is less accessible and harder to leverage than structured, discrete data. We report on an extension to the Duke Enterprise Data Unified Content Explorer (DEDUCE), a self-service query tool developed to provide clinicians and researchers with access to data within the Duke Medicine Enterprise Data Warehouse (EDW). The DEDUCE Clinical Text module supports ontology-based text searching, enhanced filtering capabilities based on document attributes, and integration of clinical text with structured data and cohort development. The module is implemented with open-source tools extensible to other institutions, including a Java-based search engine (Apache Solr) with complementary full-text indexing library (Lucene) employed with a negation engine (NegEx) modified by clinical users to include to local domain-specific negation phrases.

  10. The antibiotic Furvina® targets the P-site of 30S ribosomal subunits and inhibits translation initiation displaying start codon bias

    PubMed Central

    Fabbretti, Attilio; Brandi, Letizia; Petrelli, Dezemona; Pon, Cynthia L.; Castañedo, Nilo R.; Medina, Ricardo; Gualerzi, Claudio O.

    2012-01-01

    Furvina®, also denominated G1 (MW 297), is a synthetic nitrovinylfuran [2-bromo-5-(2-bromo-2-nitrovinyl)-furan] antibiotic with a broad antimicrobial spectrum. An ointment (Dermofural®) containing G1 as the only active principle is currently marketed in Cuba and successfully used to treat dermatological infections. Here we describe the molecular target and mechanism of action of G1 in bacteria and demonstrate that in vivo G1 preferentially inhibits protein synthesis over RNA, DNA and cell wall synthesis. Furthermore, we demonstrate that G1 targets the small ribosomal subunit, binds at or near the P-decoding site and inhibits its function interfering with the ribosomal binding of fMet-tRNA during 30S initiation complex (IC) formation ultimately inhibiting translation. Notably, this G1 inhibition displays a bias for the nature (purine vs. pyrimidine) of the 3′-base of the codon, occurring efficiently only when the mRNA directing 30S IC formation and translation contains the canonical AUG initiation triplet or the rarely found AUA triplet, but hardly occurs when the mRNA start codon is either one of the non-canonical triplets AUU or AUC. This codon discrimination by G1 is reminiscent, though of opposite type of that displayed by IF3 in its fidelity function, and remarkably does not occur in the absence of this factor. PMID:22941660

  11. Development of a long-pulse (30-s), high-energy (120-keV) ion source for neutral-beam applications

    SciTech Connect

    Tsai, C.C.; Barber, G.C.; Blue, C.W.

    1983-01-01

    Multimegawatt neutral beams of hydrogen or deuterium atoms are needed for fusion machine applications such as MFTB-B, TFTR-U, DIII-U, and FED (INTOR or ETR). For these applications, a duoPIGatron ion source is being developed to produce high-brightness deuterium beams at a beam energy of approx. 120 keV for pulse lengths up to 30 s. A long-pulse plasma generator with active water cooling has been operated at an arc level of 1200 A with 30-s pulse durations. The plasma density and uniformity are sufficient for supplying a 60-A beam of hydrogen ions to a 13- by 43-cm accelerator. A 10- by 25-cm tetrode accelerator has been operated to form 120-keV hydrogen ion beams. Using the two-dimensional (2-D) ion extraction code developed at Oak Ridge National Laboratory (ORNL), a 13- by 43-cm tetrode accelerator has been designed and is being fabricated. The aperture shapes of accelerator grids are optimized for 120-keV beam energy.

  12. CMB and the elementary particles structure deduced from QFT of non-dot model

    NASA Astrophysics Data System (ADS)

    Chen, Shao-Guang

    In my paper ‘Planck Constant Deduced from Metrical Results of Doppler Effect of Moving Particle —Uncertainty Principle Caused by Collision of a Particle with CMB Photons and Virtual Photons (H05-0036-10)’ the absolute velocity is decided by CMB which as a mark of the vacuum. CMB come from the thermal radiation of stars via gravitational redshift about 10 (13) year (E14- 0032-08). In my paper ‘Quanta turn-advance ism, China Science && Technology Overview 131 192-210 (2011)’, QFT four-dimensional uncertainty principle and momentum-energy conservation law had been generalized as a five-dimensional equations: de Broglie wavelength as a position vector \\underline{q}= (i c t, r, s), momentum \\underline{P} = (i E / c, P, U c), \\underline{q} = i h / \\underline{P}, \\underline{q} \\underline{q} = 0, \\underline{P} \\underline{P} = 0, Sigma∑ \\underline{P} = \\underline{P} (0) . The five-dimensional time-space-spin had been quantized as a non-dot model basic cell, the lowest energy state vertical polarized left spin 1/2 neutrino and right spin 1/2 antineutrino are just the left, right advance unit quanta _{0}nuυ, nuυ _{0} and left, right back unit quanta (0) nuυ, nuυ (0) , it again compose into spin 1 unit advance photons _{0}nuυnuυ _{0} and back (0) nuυnuυ (0) , spin 0 unit rest mass nuυ _{0}nuυ (0) and anti-mass _{0}nuυ (0) nuυ, spin 0 unit positive charge _{0}nuυnuυ (0) and negative charge nuυ _{0} (0) nuυ. It accord to the high energy physics experimental results of the transformation among the photons, masses quanta and charges quanta. The physical vacuum is the even collocation of non-combinational nuυ _{0} or _{0}nuυ. QFT is no longer with divergence difficulty by the non-dot model. It is mathematically easy that from five-dimensional equations deduce out the Dirac, Klein-Gordan, Maxwell equations and Lorentz force formula, but appear some new results. The interactions between _{0}nuυ, nuυ _{0}, (0) nuυ, nuυ (0) , i.e., force f

  13. Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser.

    PubMed

    Demirci, Hasan; Sierra, Raymond G; Laksmono, Hartawan; Shoeman, Robert L; Botha, Sabine; Barends, Thomas R M; Nass, Karol; Schlichting, Ilme; Doak, R Bruce; Gati, Cornelius; Williams, Garth J; Boutet, Sébastien; Messerschmidt, Marc; Jogl, Gerwald; Dahlberg, Albert E; Gregory, Steven T; Bogan, Michael J

    2013-09-01

    High-resolution ribosome structures determined by X-ray crystallography have provided important insights into the mechanism of translation. Such studies have thus far relied on large ribosome crystals kept at cryogenic temperatures to reduce radiation damage. Here, the application of serial femtosecond X-ray crystallography (SFX) using an X-ray free-electron laser (XFEL) to obtain diffraction data from ribosome microcrystals in liquid suspension at ambient temperature is described. 30S ribosomal subunit microcrystals diffracted to beyond 6 Å resolution, demonstrating the feasibility of using SFX for ribosome structural studies. The ability to collect diffraction data at near-physiological temperatures promises to provide fundamental insights into the structural dynamics of the ribosome and its functional complexes.

  14. A Woman in Her 30s With a Past History of HIV Disease Presented With Recurrent Fever, Night Sweats, and Small Bilateral Pulmonary Nodules.

    PubMed

    Hashmi, Hafiz Rizwan Talib; Niazi, Masooma; Adrish, Muhammad

    2016-06-01

    A woman in her 30s presented with recurrent low-grade fever and cough (onset, 1 week). She reported occasional night sweats and weight loss of approximately 20 pounds over the past 4 months. She denied nausea, vomiting, diarrhea, or any urinary complaints. Her past medical history was significant for chronic hepatitis C and HIV infection, the latter diagnosed in 2001. She was noncompliant with highly active antiretroviral therapy for more than 4 years and had pneumocystis pneumonia 2 years prior to this presentation. She had a 10-pack per year smoking history and reported active use of cocaine and heroin. The patient denied any occupational exposures. Copyright © 2016 American College of Chest Physicians. Published by Elsevier Inc. All rights reserved.

  15. Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser

    PubMed Central

    Demirci, Hasan; Sierra, Raymond G.; Laksmono, Hartawan; Shoeman, Robert L.; Botha, Sabine; Barends, Thomas R. M.; Nass, Karol; Schlichting, Ilme; Doak, R. Bruce; Gati, Cornelius; Williams, Garth J.; Boutet, Sébastien; Messerschmidt, Marc; Jogl, Gerwald; Dahlberg, Albert E.; Gregory, Steven T.; Bogan, Michael J.

    2013-01-01

    High-resolution ribosome structures determined by X-ray crystallography have provided important insights into the mechanism of translation. Such studies have thus far relied on large ribosome crystals kept at cryogenic temperatures to reduce radiation damage. Here, the application of serial femtosecond X-ray crystallography (SFX) using an X-ray free-electron laser (XFEL) to obtain diffraction data from ribosome microcrystals in liquid suspension at ambient temperature is described. 30S ribosomal subunit microcrystals diffracted to beyond 6 Å resolution, demonstrating the feasibility of using SFX for ribosome structural studies. The ability to collect diffraction data at near-physiological temperatures promises to provide fundamental insights into the structural dynamics of the ribosome and its functional complexes. PMID:23989164

  16. Mutations of ribosomal protein S5 suppress a defect in late-30S ribosomal subunit biogenesis caused by lack of the RbfA biogenesis factor

    PubMed Central

    Nord, Stefan; Bhatt, Monika J.; Tükenmez, Hasan; Farabaugh, Philip J.; Wikström, P. Mikael

    2015-01-01

    The in vivo assembly of ribosomal subunits requires assistance by maturation proteins that are not part of mature ribosomes. One such protein, RbfA, associates with the 30S ribosomal subunits. Loss of RbfA causes cold sensitivity and defects of the 30S subunit biogenesis and its overexpression partially suppresses the dominant cold sensitivity caused by a C23U mutation in the central pseudoknot of 16S rRNA, a structure essential for ribosome function. We have isolated suppressor mutations that restore partially the growth of an RbfA-lacking strain. Most of the strongest suppressor mutations alter one out of three distinct positions in the carboxy-terminal domain of ribosomal protein S5 (S5) in direct contact with helix 1 and helix 2 of the central pseudoknot. Their effect is to increase the translational capacity of the RbfA-lacking strain as evidenced by an increase in polysomes in the suppressed strains. Overexpression of RimP, a protein factor that along with RbfA regulates formation of the ribosome's central pseudoknot, was lethal to the RbfA-lacking strain but not to a wild-type strain and this lethality was suppressed by the alterations in S5. The S5 mutants alter translational fidelity but these changes do not explain consistently their effect on the RbfA-lacking strain. Our genetic results support a role for the region of S5 modified in the suppressors in the formation of the central pseudoknot in 16S rRNA. PMID:26089326

  17. The effect of acute hypoxia at low altitude and acute normoxia at high altitude on performance during a 30-s Wingate test in children.

    PubMed

    Blonc, S; Falgairette, G; Bedu, M; Fellmann, N; Spielvogel, H; Coudert, J

    1994-10-01

    The effect of acute hypoxia (FIO2 = 0.137 +/- 0.001) at Low Altitude (LA: Clermont-Ferrand, 330 m) and acute normoxia (FIO2 = 0.306 +/- 0.006) at High Altitude (HA: La Paz, 3600 m) on performance during a 30-s Wingate test has been investigated in prepubertal children (Tanner stage 1). Twenty five boys (LA, n = 10; HA, n = 15) aged from 10.6 to 12.7 years performed two Wingate tests at random: at LA, one in normoxia (ambient air) and one in acute hypoxia and at HA, one in chronic hypoxia (ambient air) and one in acute normoxia. The subjects performed the two tests using the same calibrated cycle ergometer. Peak Power (PP), Mean Power (MP), O2 uptake during the 30 s (VO2) and blood lactate accumulation (delta [L]s) were measured. Compared to normoxia, acute hypoxia at LA did not alter PP (8.0 +/- 1.1 vs 7.9 +/- 1.3 W.kg-1 BW) and MP (6.1 +/- 0.7 vs 6.1 +/- 1.1 W.kg-1 BW). Similarly, compared to chronic hypoxia, acute normoxia at HA did not modify these parameters (PP: 7.4 +/- 1.5 vs 7.3 +/- 1.8; MP: 5.4 +/- 1.2 vs 5.5 +/- 1.1; W.kg-1 BW). VO2 and delta [L]s were neither significantly changed by acute hypoxia at LA (520 +/- 50 vs 550 +/- 60 ml O2; 5.3 +/- 1.7 vs 4.8 +/- 1.7 mmol.l-1) nor by acute normoxia at HA (530 +/- 110 vs 500 +/- 90 ml O2; 3.4 +/- 1.3 vs 3.3 +/- 1.0 mmol.l-1).(ABSTRACT TRUNCATED AT 250 WORDS)

  18. Deducing the electron-beam diameter in a laser-plasma accelerator using x-ray betatron radiation.

    PubMed

    Schnell, Michael; Sävert, Alexander; Landgraf, Björn; Reuter, Maria; Nicolai, Maria; Jäckel, Oliver; Peth, Christian; Thiele, Tobias; Jansen, Oliver; Pukhov, Alexander; Willi, Oswald; Kaluza, Malte C; Spielmann, Christian

    2012-02-17

    We investigate the properties of a laser-plasma electron accelerator as a bright source of keV x-ray radiation. During the interaction, the electrons undergo betatron oscillations and from the carefully measured x-ray spectrum the oscillation amplitude of the electrons can be deduced which decreases with increasing electron energies. From the oscillation amplitude and the independently measured x-ray source size of (1.8±0.3) μm we are able to estimate the electron bunch diameter to be (1.6±0.3) μm.

  19. Adult Opisthorchis felineus major protein fractions deduced from transcripts: comparison with liver flukes Opisthorchis viverrini and Clonorchis sinensis.

    PubMed

    Pomaznoy, Mikhail; Tatkov, Sergey; Katokhin, Alexey; Afonnikov, Dmitry; Babenko, Vladimir; Furman, Dagmara; Brusentsov, Ilya; Belavin, Pavel; Najakshin, Alexandr; Guselnikov, Sergey; Vasiliev, Gennady; Sivkov, Anton; Prokhortchouk, Egor; Skryabin, Konstantin; Mordvinov, Viatcheslav

    2013-10-01

    The epidemiologically important liver flukes Opisthorchis felineus, Opisthorchis viverrini, and Clonorchis sinensis are of interest to health professionals, epidemiologists, pharmacologists, and molecular biologists. Recently the transcriptomes of the latter two species were intensively investigated. However our knowledge on molecular biology of O. felineus is scarce. We report the first results of the O. felineus transcriptome analysis. We isolated and annotated a total of 2560 expressed sequence tag (EST) sequences from adult O. felineus (deposited within the database of expressed sequence tags (dbEST), under accession numbers GenBank: JK624271-JK626790, JK006511-JK006547, JK649790-JK649792). Clustering and analysis resulted in the detection of 267 contigs. Of the protein sequences deduced from these, 82% had homologs in the NCBI (nr) protein database and 63% contained conserved domains, allowing the functions to be interpreted using the Gene Ontology terms. Comprehensive analysis of Opisthorchiidae- and Trematoda-specific substitutions within amino acid sequences deduced for the proteins myoglobin, vitelline precursor protein, cathepsin F, and 28kDa glutathione transferase was carried out. The gene set of the 32 ribosomal proteins for the three Opisthorchiidae species with the addition of available Schistosoma and Fasciola orthologs was created and is provided in the supplementary. The orthologous gene set created was used for inferring phylogeny within the Trematoda with special attention to interrelations within the Opisthorchiidae. The phylogenetic analysis revealed a closer relationship between C. sinensis and O. viverrini and some divergence of O. felineus from either O. viverrini or C. sinensis.

  20. The translational fidelity function of IF3 during transition from the 30 S initiation complex to the 70 S initiation complex.

    PubMed

    Grigoriadou, Christina; Marzi, Stefano; Pan, Dongli; Gualerzi, Claudio O; Cooperman, Barry S

    2007-10-26

    IF3 has a fidelity function in the initiation of translation, inducing the dissociation of fMet-tRNA(fMet) from the 30 S initiation complexes (30SIC) containing a non-canonical initiation triplet (e.g. AUU) in place of a canonical initiation triplet (e.g., AUG). IF2 has a complementary role, selectively promoting initiator tRNA binding to the ribosome. Here, we used parallel rapid kinetics measurements of GTP hydrolysis, Pi release, light-scattering, and changes in intensities of fluorophore-labeled IF2 and fMet-tRNA(fMet) to determine the effects on both 30SIC formation and 30SIC conversion to 70 S initiation complexes (70SIC) of (a) substituting AUG with AUU, and/or (b) omitting IF3, and/or (c) replacing GTP with the non-hydrolyzable analog GDPCP. We demonstrate that the presence or absence of IF3 has, at most, minor effects on the rate of 30SIC formation using either AUG or AUU as the initiation codon, and conclude that the high affinity of IF2 for both 30 S subunit and initiator tRNA overrides any perturbation of the codon-anticodon interaction resulting from AUU for AUG substitution. In contrast, replacement of AUG by AUU leads to a dramatic reduction in the rate of 70SIC formation from 30SIC upon addition of 50 S subunits. Interpreting our results in the framework of a quantitative kinetic scheme leads to the conclusion that, within the overall process of 70SIC formation, the step most affected by substituting AUU for AUG involves the conversion of an initially labile 70 S ribosome into a more stable complex. In the absence of IF3, the difference between AUG and AUU largely disappears, with each initiation codon affording rapid 70SIC formation, leading to the hypothesis that it is the rate of IF3 dissociation from the 70 S ribosome during IC70S formation that is critical to its fidelity function.

  1. Lithium- and boron-bearing brines in the Central Andes: exploring hydrofacies on the eastern Puna plateau between 23° and 23°30'S

    NASA Astrophysics Data System (ADS)

    Steinmetz, R. L. López

    2017-01-01

    Internally drained basins of the Andean Plateau are lithium- and boron-bearing systems. The exploration of ionic facies and parental links in a playa lake located in the eastern Puna (23°-23°30'S) was assessed by hydrochemical determinations of residual brines, feed waters and solutions from weathered rocks. Residual brines have been characterized by the Cl- (SO4 =)/Na+ (K+) ratio. Residual brines from the playa lake contain up to 450 mg/l of boron and up to 125 mg/l of lithium, and the Las Burras River supplies the most concentrated boron (20 mg/l) and lithium (3.75 mg/l) inflows of the basin. The hydro-geochemical assessment allowed for the identification of three simultaneous sources of boron: (1) inflow originating from granitic areas of the Aguilar and Tusaquillas ranges; (2) weathering of the Ordovician basement; and (3) boron-rich water from the Las Burras River. Most of the lithium input of the basin is likely generated by present geothermal sources rather than by weathering and leaching of ignimbrites and plutonic rocks. However, XRD analyses of playa lake sediments revealed the presence of lithian micas of clastic origin, including taeniolite and eucriptite. This study is the first to document these rare Li-micas from the Puna basin. Thus, both residual brines and lithian micas contribute to the total Li content in the studied hydrologic system.

  2. The interstellar redding law in the ultraviolet deduced from filter photometry obtained by the OAO-2 satellite

    NASA Technical Reports Server (NTRS)

    Laget, M.

    1972-01-01

    Filter photometry has been obtained of 16 BO stars at ten effective wavelengths in the range 4250-1430 A. The wavelength dependence of the interstellar reddening law, deduced from a least squares fit of the observed values to the reddening line at each band, is found in satisfactory agreement with that derived by Bless and Savage (1972). Toward the shorter wavelengths the increase of the computed probable error of the slope of the mean reddening line suggests that large fluctuations in the law may occur from star to star. Similar computations, separating main-sequence stars and supergiants, indicate that the large fluctuations of the law appear to be well related to the luminosity of the stars; the supergiants show systematically less extinction, this deficiency becoming large toward the far UV. The small number in the sample however, does not allow a general conclusion to be drawn.

  3. Intra-plasmaspheric wave power density deduced from long-term DEMETER measurements of terrestrial VLF transmitter wave amplitudes

    NASA Astrophysics Data System (ADS)

    Lauben, D.; Cohen, M.; Inan, U.

    2012-12-01

    We deduce the 3d intra-plasmaspheric distribution of VLF wave power between conjugate regions of strong VLF wave amplitudes as measured by DEMETER for high-power terrestrial VLF transmitters during its ~6-yr lifetime. We employ a mixed WKB/full-wave technique to solve for the primary and secondary electromagnetic and electrostatic waves which are transmitted and reflected from strong cold-plasma density gradients and posited irregularities, in order to match the respective end-point measured amplitude distributions. Energy arriving in the conjugate region and also escaping to other regions of the magnetosphere is note. The resulting 3d distribution allows improved estimates for the long-term average particle scattering induced by terrestrial VLF transmitters.

  4. Parametric model of the Mueller matrix of a Spectralon white reflectance standard deduced by polar decomposition techniques.

    PubMed

    Kildemo, Morten; Maria, Jérôme; Ellingsen, Pål G; Aas, Lars M S

    2013-07-29

    Decomposition methods have been applied to in-plane Mueller matrix ellipsometric scattering data of the Spectralon reflectance standard. Data were measured at the wavelengths 532 nm and 1500 nm, using an achromatic optimal Mueller matrix scatterometer applying a photomultiplier tube and a high gain InGaAs detector for the two wavelengths. A parametric model with physical significance was deduced through analysis of the product decomposed matrices. It is found that when the data are analyzed as a function of the scattering angle, similar to particle scattering, the matrix elements are largely independent of incidence angle. To the first order, we propose that a Guassian lineshape is appropriate to describe the polarization index, while the decomposed diagonal elements of the retardance matrix have a form resembling Rayleigh single scattering. New models are proposed for the off diagonal elements of the measured Mueller matrix.

  5. Fracture-mechanics data deduced from thermal-shock and related experiments with LWR pressure-vessel material

    SciTech Connect

    Cheverton, R.D.; Canonico, D.A.; Iskander, S.K.; Bolt, S.E.; Holz, P.P.; Nanstad, R.K.; Stelzman, W.J.

    1982-01-01

    Pressurized water reactors (PWRs) are susceptible to certain types of hypothetical accidents that can subject the reactor pressure vessel to severe thermal shock, that is, a rapid cooling of the inner surface of the vessel wall. The thermal-shock loading, coupled with the radiation-induced reduction in the material fracture toughness, introduces the possibility of propagation of preexistent flaws and what at one time were regarded as somewhat unique fracture-oriented conditions. Several postulated reactor accidents have been analyzed to discover flaw behavior trends; seven intermediate-scale thermal-shock experiments with steel cylinders have been conducted; and corresponding materials characterization studies have been performed. Flaw behavior trends and related fracture-mechanics data deduced from these studies are discussed.

  6. Submarine paleoseismology of the northern Hikurangi subduction margin of New Zealand as deduced from Turbidite record since 16 ka

    NASA Astrophysics Data System (ADS)

    Pouderoux, Hugo; Proust, Jean-Noël; Lamarche, Geoffroy

    2014-01-01

    Paleoseismic studies seek to characterise the signature of pre-historical earthquakes by deriving quantitative information from the geological record such as the source, magnitude and recurrence of moderate to large earthquakes. In this study, we provide a ˜16,000 yr-long paleo-earthquake record of the 200 km-long northern Hikurangi Margin, New Zealand, using cm-thick deep-sea turbidites identified in sediment cores. Cores were collected in strategic locations across the margin within three distinct morphological re-entrants - the Poverty, Ruatoria and Matakaoa re-entrants. The turbidite facies vary from muddy to sandy with evidence for rare hyperpycnites interbedded with hemipelagites and tephra. We use the Oxal probabilistic software to model the age of each turbidite, using the sedimentation rate of hemipelagite deduced from well-dated tephra layers and radiocarbon ages measurements on planktonic foraminifera.

  7. A blind trial evaluation of a crime scene methodology for deducing impact velocity and droplet size from circular bloodstains.

    PubMed

    Hulse-Smith, Lee; Illes, Mike

    2007-01-01

    In a previous study, mechanical engineering models were utilized to deduce impact velocity and droplet volume of circular bloodstains by measuring stain diameter and counting spines radiating from their outer edge. A blind trial study was subsequently undertaken to evaluate the accuracy of this technique, using an applied, crime scene methodology. Calculations from bloodstains produced on paper, drywall, and wood were used to derive surface-specific equations to predict 39 unknown mock crime scene bloodstains created over a range of impact velocities (2.2-5.7 m/sec) and droplet volumes (12-45 microL). Strong correlations were found between expected and observed results, with correlation coefficients ranging between 0.83 and 0.99. The 95% confidence limit associated with predictions of impact velocity and droplet volume was calculated for paper (0.28 m/sec, 1.7 microL), drywall (0.37 m/sec, 1.7 microL), and wood (0.65 m/sec, 5.2 microL).

  8. Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome

    PubMed Central

    Udaondo, Zulema; Molina, Lazaro; Daniels, Craig; Gómez, Manuel J; Molina-Henares, María A; Matilla, Miguel A; Roca, Amalia; Fernández, Matilde; Duque, Estrella; Segura, Ana; Ramos, Juan Luis

    2013-01-01

    Summary Pseudomonas putida DOT-T1E is an organic solvent tolerant strain capable of degrading aromatic hydrocarbons. Here we report the DOT-T1E genomic sequence (6 394 153 bp) and its metabolic atlas based on the classification of enzyme activities. The genome encodes for at least 1751 enzymatic reactions that account for the known pattern of C, N, P and S utilization by this strain. Based on the potential of this strain to thrive in the presence of organic solvents and the subclasses of enzymes encoded in the genome, its metabolic map can be drawn and a number of potential biotransformation reactions can be deduced. This information may prove useful for adapting desired reactions to create value-added products. This bioengineering potential may be realized via direct transformation of substrates, or may require genetic engineering to block an existing pathway, or to re-organize operons and genes, as well as possibly requiring the recruitment of enzymes from other sources to achieve the desired transformation. Funding Information Work in our laboratory was supported by Fondo Social Europeo and Fondos FEDER from the European Union, through several projects (BIO2010-17227, Consolider-Ingenio CSD2007-00005, Excelencia 2007 CVI-3010, Excelencia 2011 CVI-7391 and EXPLORA BIO2011-12776-E). PMID:23815283

  9. Morphology of high-latitude plasma density perturbations as deduced from the total electron content measurements onboard the Swarm constellation

    NASA Astrophysics Data System (ADS)

    Park, Jaeheung; Lühr, Hermann; Kervalishvili, Guram; Rauberg, Jan; Stolle, Claudia; Kwak, Young-Sil; Lee, Woo Kyoung

    2017-01-01

    In this study, we investigate the climatology of high-latitude total electron content (TEC) variations as observed by the dual-frequency Global Navigation Satellite Systems (GNSS) receivers onboard the Swarm satellite constellation. The distribution of TEC perturbations as a function of geographic/magnetic coordinates and seasons reasonably agrees with that of the Challenging Minisatellite Payload observations published earlier. Categorizing the high-latitude TEC perturbations according to line-of-sight directions between Swarm and GNSS satellites, we can deduce their morphology with respect to the geomagnetic field lines. In the Northern Hemisphere, the perturbation shapes are mostly aligned with the L shell surface, and this anisotropy is strongest in the nightside auroral (substorm) and subauroral regions and weakest in the central polar cap. The results are consistent with the well-known two-cell plasma convection pattern of the high-latitude ionosphere, which is approximately aligned with L shells at auroral regions and crossing different L shells for a significant part of the polar cap. In the Southern Hemisphere, the perturbation structures exhibit noticeable misalignment to the local L shells. Here the direction toward the Sun has an additional influence on the plasma structure, which we attribute to photoionization effects. The larger offset between geographic and geomagnetic poles in the south than in the north is responsible for the hemispheric difference.

  10. Evolutionary relationships of a plant-pathogenic mycoplasmalike organism and Acholeplasma laidlawii deduced from two ribosomal protein gene sequences.

    PubMed Central

    Lim, P O; Sears, B B

    1992-01-01

    The families within the class Mollicutes are distinguished by their morphologies, nutritional requirements, and abilities to metabolize certain compounds. Biosystematic classification of the plant-pathogenic mycoplasmalike organisms (MLOs) has been difficult because these organisms have not been cultured in vitro, and hence their nutritional requirements have not been determined nor have physiological characterizations been possible. To investigate the evolutionary relationship of the MLOs to other members of the class Mollicutes, a segment of a ribosomal protein operon was cloned and sequenced from an aster yellows-type MLO which is pathogenic for members of the genus Oenothera and from Acholeplasma laidlawii. The deduced amino acid sequence data from the rpl22 and rps3 genes indicate that the MLOs are more closely related to A. laidlawii than to animal mycoplasmas, confirming previous results from 16S rRNA sequence comparisons. This conclusion is also supported by the finding that the UGA codon is not read as a tryptophan codon in the MLO and A. laidlawii, in contrast to its usage in Mycoplasma capricolum. PMID:1556079

  11. Confidence envelop of the global MSL time series deduced from TOPEX, Jason-1 and Jason-2 altimeter missions.

    NASA Astrophysics Data System (ADS)

    Legeais, JeanFrancois; Ablain, Michael; Zawadzki, Lionel

    2015-04-01

    With the satellite altimetry missions, the global mean sea level (GMSL) has been calculated on a continual basis since January 1993. 'Verification' phases, during which the satellites follow each other in close succession (TOPEX/Poseidon--Jason-1, then Jason-1--Jason-2), help to link up these different missions by precisely determining any bias between them. The global mean sea level (MSL) deduced from these 3 altimetric missions provides a global rate of 3.2 mm from 1993 to 2013 applying the post glacial rebound (MSL AVISO website http://www.jason.oceanobs.com/msl). Within the ESA Climate Change Initiative program, the users requirements have been collected and for the users of the Sea Level ECV, it is crucial to know as much as possible the errors impacting the MSL calculation in order to analyze the MSL variations and in fine to interpret correctly the geophysical mechanisms underlying these variations. The characterization of these errors was performed over the whole altimetric period separating several time scales as the long-term evolution (mean sea level trend), but also the inter-annual and periodic signals. However, it will also be very useful to provide the confidence envelop (or error envelop) of the global MSL time series in order to know the exact error level at each time step. In this paper, we propose to describe in details the approach developed to compute this confidence envelop. We will also present the results obtained and how to interpret them.

  12. Isolation, sequencing and expression of Bartonella henselae omp43 and predicted membrane topology of the deduced protein.

    PubMed

    Burgess, A W; Paquet, J Y; Letesson, J J; Anderson, B E

    2000-08-01

    The infection of and interaction of human endothelial cells with Bartonella henselae is one of the most interesting aspects of Bartonella -associated disease. The gene encoding the 43 kDa B. henselae outer membrane protein (Omp43) that binds endothelial cells was cloned and sequenced. Sequence analysis revealed an open reading frame of 1206 nucleotides coding for a protein of 402 amino acids. Analysis of the deduced amino acid sequence shows 38% identity over the entire sequence to the Brucella spp. In addition to this Omp2b porin also shows a signal sequence and peptidase cleavage site. Cleavage of the signal peptide results in a mature 380 amino acid polypeptide with a predicted molecular weight of 42 kDa. Omp43 was expressed in Escherichia coli as a fusion protein. Purified recombinant Omp43 at concentrations of 11 and 2.75 microg/ml bound to intact human umbilical vein endothelial cells. Membrane topology analysis predicts that Omp43 exists as a 16 stranded beta barrel protein, similar to that predicted for the Omp2b Brucella abortus porin. Characterization and expression of the gene encoding Omp43 should provide a tool for further investigation of the role of adherence to endothelial cells in the pathogenesis of B. henselae. Copyright 2000 Academic Press.

  13. Deducing solid liquid interfacial energy from superheating or supercooling: application to H2O at high pressures

    NASA Astrophysics Data System (ADS)

    Luo, Sheng-Nian; Strachan, Alejandro; Swift, Damian C.

    2005-04-01

    We present a general method to determine the solid-liquid interfacial energy (γsl) from the maximum supercooling (or superheating), and apply it to the water-ice system. For solid-liquid phase transitions, the nucleation-theory-based systematics of maximum superheating and supercooling relate a dimensionless nucleation barrier to the superheating (supercooling) and heating (cooling) rates. Given superheating (or supercooling) values from either experiments or simulations, γsl can then be deduced from the dimensionless nucleation barrier, equilibrium melting temperature and enthalpy of fusion. We demonstrate the accuracy of this approach using molecular dynamics (MD) simulations of the Lennard-Jones system: our predictions of γsl at various pressures are in excellent agreement with independent, direct MD simulations. With this approach, we predict γsl for the water-ice (Ih and III) system using experimental supercooling values in the pressure range of 0-0.3 GPa. The predicted value (28 ± 0.8 mJ m-2) agrees with measurements on H2O-Ih at ambient pressure.

  14. Deducing the stress-strain response of anisotropic Zr-2.5%Nb pressure tubing by spherical indentation testing

    NASA Astrophysics Data System (ADS)

    Oviasuyi, R. O.; Klassen, R. J.

    2013-01-01

    In this paper we developed a method of analysis by which the average stress-plastic strain flow curve of mechanically anisotropic materials can be deduced from spherical indentation test data. Our analysis is based upon spherical indentation tests performed on the extruded and cold-drawn Zr-2.5%Nb CANDU pressure tube material over the range of temperature from 25 °C to 300 °C. The indentation force and depth data were analyzed and σavg and ɛavg were calculated using previously reported equations developed for spherical indentation of isotropic material which were then modified, by incorporating the appropriate Hill's anisotropy coefficients, to characterize the anisotropic yield stress of the indented material. The resulting flow curves were dependent on indentation direction and correspond closely with flow curves obtained from previously reported conventional uniaxial stress tests performed on the Zr-2.5%Nb material. Indentation tests performed with large, 200 μm, and small, 40 μm, diameter spheres indicate that for small diameter indentations, when the indentation depth is less than several micrometers, the calculated σavg is heavily influenced by the depth dependence of the yield strength of the indented material.

  15. Deducing receptor signaling parameters from in vivo analysis: LuxN/AI-1 quorum sensing in Vibrio harveyi

    PubMed Central

    Swem, Lee R.; Swem, Danielle L.; Wingreen, Ned S.; Bassler, Bonnie L.

    2008-01-01

    Summary Quorum sensing, a process of bacterial cell-cell communication, relies on production, detection, and response to autoinducer signaling molecules. Here we focus on LuxN, a nine transmembrane domain protein from Vibrio harveyi, and the founding example of membrane-bound receptors for acyl-homoserine lactone (AHL) autoinducers. Previously, nothing was known about signal recognition by membrane-bound AHL receptors. We used mutagenesis and suppressor analyses to identify the AHL-binding domain of LuxN, and discovered LuxN mutants that confer decreased and increased AHL sensitivity. Our analysis of dose-response curves of multiple LuxN mutants pins these inverse phenotypes on quantifiable opposing shifts in the free-energy bias of LuxN for its kinase and phosphatase states. To extract signaling parameters, we exploited a strong LuxN antagonist, one of fifteen small-molecule antagonists we identified. We find that quorum-sensing-mediated communication can be manipulated positively and negatively to control bacterial behavior, and that signaling parameters can be deduced from in vivo data. PMID:18692469

  16. New prospects for deducing the evolutionary history of metabolic pathways in prokaryotes: Aromatic biosynthesis as a case-in-point

    NASA Astrophysics Data System (ADS)

    Ahmad, Suhail; Jensen, Roy A.

    1988-03-01

    Metabolic pathways of prokaryotes are more biochemically diverse than is generally recognized. Distinctive biochemical features are shared by phylogenetic clusters. The hierarchical levels of characterstate clustering depends upon evolutionary events which fortuitously became fixed in the genome of a common ancestor. Prokaryotes can now be ordered on a phylogenetic tree. This allows the evolutionary steps that underlie the construction and regulation of appropriately complex biochemical pathways to be traced in an evolutionary progression of prokaryote types that house these pathways. Essentially the approach is to deduce ancestral character states at ever deeper phylogenetic levels, utilizing logical principles of maximum parsimony. The current perspective on the evolution of the biochemical pathway for biosynthesis of aromatic amino acids is developed as a case-in-point model for analyses that should be feasible with many major metabolic systems. Phenylalanine biosynthesis probably arose prior to the addition of branches leading to tyrosine and tryptophan. An evolutionary scenario is developed that begins with non-enzymatic reactions which may have operated in primitive systems, followed by the evolution of an enzymatic system that pre-dated the divergence of major lineages of modern eubacteria (Gram-positive bacteria, Gram-negative purple bacteria, and cyanobacteria).

  17. BASE COMPOSITION OF DEOXYRIBONUCLEIC ACID OF SULFATE-REDUCING BACTERIA DEDUCED FROM BUOYANT DENSITY MEASUREMENTS IN CESIUM CHLORIDE

    PubMed Central

    Saunders, Grady F.; Campbell, L. Leon; Postgate, John R.

    1964-01-01

    Saunders, Grady F. (University of Illinois, Urbana), L. Leon Campbell, and John R. Postgate. Base composition of deoxyribonucleic acid of sulfate-reducing bacteria deduced from buoyant density measurements in cesium chloride. J. Bacteriol. 87:1073–1078. 1964.—The base composition of the deoxyribonucleic acid (DNA) of sulfate-reducing bacteria was calculated from buoyant density measurements in CsCl. The sporulating sulfate-reducing bacteria fell into two groups: Desulfovibrio orientis with a DNA base composition of 42% guanine plus cytosine (G + C), and Clostridium nigrificans with a DNA base composition of 45% G + C. The mesophilic relative of C. nigrificans had a DNA base composition of 46% G + C. Thirty strains of nonsporulating sulfate-reducing bacteria called D. desulfuricans were studied. They fell into three groups as judged by DNA base composition: group I (11 strains), 60 to 62% G + C; group II (13 strains), 54 to 56% G + C; and group III (6 strains), 46 to 47% G + C. These data underline the need for a taxonomic revision of this group of microorganisms. PMID:5874533

  18. Deducing the 236Pu(n,f) and 237Pu(n,f) cross sections via the surrogate ratio method

    NASA Astrophysics Data System (ADS)

    Hughes, R. O.; Beausang, C. W.; Ross, T. J.; Burke, J. T.; Casperson, R. J.; McCleskey, M.; Cooper, N.; Escher, J. E.; Gell, K. B.; Good, E.; Humby, P.; Saastimoinen, A.; Tarlow, T. D.

    2013-10-01

    The short half-lives associated with certain minor actinide nuclei that are relevant to stockpile stewardship pursuits and the development of next-generation nuclear reactors make direct neutron measurements very challenging. In certain cases, a stable beam and target ``surrogate reaction'' can be used in lieu of the neutron-induced reaction, and the (n,f) cross section can then be deduced indirectly. Agreement between surrogate and direct measurements for (n,f) cross sections in actinide nuclei is usually within 10%. The present work reports on the measurement of the 236Pu(n,f) and 237Pu(n,f) cross sections via 239Pu(p,tf) and 239Pu(p,df) surrogate reactions, respectively. The experiment was performed at the Texas A&M University Cyclotron Facility using a 28.5 MeV proton beam to bombard 239Pu and 235U targets. Outgoing light ions were detected in coincidence with fission fragments using the STAR-LiTe detector array. Results of the analysis will be presented. This work was supported by DoE Grant Numbers: DE-FG52-09NA29454 and DE-FG02-05ER41379 (Richmond), DE-AC52-07NA27344 (LLNL) and DE-FG52-09NA29467 (TAMU).

  19. Deducing Hybrid Performance from Parental Metabolic Profiles of Young Primary Roots of Maize by Using a Multivariate Diallel Approach

    PubMed Central

    Feher, Kristen; Lisec, Jan; Römisch-Margl, Lilla; Selbig, Joachim; Gierl, Alfons; Piepho, Hans-Peter; Nikoloski, Zoran; Willmitzer, Lothar

    2014-01-01

    Heterosis, the greater vigor of hybrids compared to their parents, has been exploited in maize breeding for more than 100 years to produce ever better performing elite hybrids of increased yield. Despite extensive research, the underlying mechanisms shaping the extent of heterosis are not well understood, rendering the process of selecting an optimal set of parental lines tedious. This study is based on a dataset consisting of 112 metabolite levels in young roots of four parental maize inbred lines and their corresponding twelve hybrids, along with the roots' biomass as a heterotic trait. Because the parental biomass is a poor predictor for hybrid biomass, we established a model framework to deduce the biomass of the hybrid from metabolite profiles of its parental lines. In the proposed framework, the hybrid metabolite levels are expressed relative to the parental levels by incorporating the standard concept of additivity/dominance, which we name the Combined Relative Level (CRL). Our modeling strategy includes a feature selection step on the parental levels which are demonstrated to be predictive of CRL across many hybrid metabolites. We demonstrate that these selected parental metabolites are further predictive of hybrid biomass. Our approach directly employs the diallel structure in a multivariate fashion, whereby we attempt to not only predict macroscopic phenotype (biomass), but also molecular phenotype (metabolite profiles). Therefore, our study provides the first steps for further investigations of the genetic determinants to metabolism and, ultimately, growth. Finally, our success on the small-scale experiments implies a valid strategy for large-scale experiments, where parental metabolite profiles may be used together with profiles of selected hybrids as a training set to predict biomass of all possible hybrids. PMID:24409329

  20. Light regulation of cGMP metabolism in toad rod outer segments (ROS) deduced from intact photoreceptor and cellfree kinetics

    SciTech Connect

    Dawis, S.M.; Graeff, R.M.; Heyman, R.A.; Walseth, T.F.; Butz, E.A.

    1987-05-01

    The rate of cGMP hydrolysis by phosphodiesterase (PDE) in intact ROS, monitored in dark-adapted isolated toad retina by the rate of /sup 18/O appearance in guanine nucleotide ..cap alpha..-phosphoryls, is 1/360th of that observed in disrupted ROS at a substrate concentration equivalent to the total (cGMP) in ROS. Low to moderate photic stimuli increase this cGMP hydrolytic rate up to 10-fold in intact ROS with little or no change in total (cGMP). G-protein activation determined in intact ROS by the fraction of GDP labeled with /sup 18/O corresponds with light-related increases in cGMP flux. In contrast, relatively high intensities and extended illumination cause attenuation of maximal cGMP hydrolysis with proportionate reductions in total (cGMP). From these observations combined with the effects of activated G-protein on kinetics and cGMP binding of ROS PDE the following model for light-regulation of cGMP metabolism was deduced: cGMP flux in intact ROS is severely restricted in the dark state because approximately 99% of the cGMP is bound to high affinity sites on the non-stimulated form of PDE. This constraint is relieved when activated G-protein converts the cGMP-binding form of PDE to a high K/sub m/ catalytic form. cGMP is then redistributed to a dynamic pool where it is available to PDE catalytic sites and lower affinity allosteric sites. The (cGMP) in the dynamic pool is maintained or further increased or decreased by modulating the activity of an apparently light-sensitive guanylyl cyclase.

  1. Implications of Late Pliocene-Pleistocene Humidity Fluctuations in the Qaidam Paleolake (NE Tibetan Plateau) Deduced from Magnetic Susceptibility Measurements

    NASA Astrophysics Data System (ADS)

    Herb, C.; Appel, E.; Koutsodendris, A.; Zhang, W.; Pross, J.; Fang, X.

    2014-12-01

    The Qaidam Basin (NE Tibetan Plateau) contains a near-continuous, up to 12 km thick sequence of Cenozoic strata that offers a unique opportunity for studying long-term climate change. We investigate the 940-m-long drill core SG-1 from the western Qaidam Basin, which is characterized by a long-term transition from a semi-deep freshwater lake to nearly complete exsiccation of the water body, detected by several studies including geochemical and lithological observations. Based on magnetostratigraphy and optically stimulated luminescence dating, and refined by orbital tuning, the SG-1 core spans the interval from 2.69 to 0.1 Ma. Moisture availability in the western Qaidam Basin deduced from the pollen ratio Artemisia/Chenopodiaceae (A/C), suggests desert to steppe vegetation along core SG-1 as a long-term feature. Magnetic susceptibility (χ) is well suited for the high-resolution investigation of paleohumidity. The meaning of χ as a paleohydrology proxy is shown by comparing χ to other magnetic proxies for checking its relation to magnetic grain sizes and magnetic mineralogy as well as to pollen results. χ variations are analyzed to obtain regional information on the factors leading to the drying process of the Qaidam paleolake as well as potential driving factors for humidity fluctuations (e.g., insolation). An important topic that needs further investigation is the influence of monsoon in the Qaidam Basin. While the southern part of the Tibetan Plateau is directly affected by monsoon precipitation through the topographic barrier, its influence in the past is questionable in the hyper-arid Qaidam Basin. We check a potential coupling to the monsoon system in the western Qaidam Basin by comparing our χ record to reconstructions of the Asian monsoon system from other archives as well as searching evidence from orbital cyclicities found in the χ time series.

  2. Evaluation des programmes de techniques d'education en services de garde (Evaluation of Programs of Educational Methods in Child Care Services. 2410-0521.

    ERIC Educational Resources Information Center

    Vigneau, Paul, Comp.

    In March 1994, Quebec's Commission on the Evaluation of Collegiate Teaching initiated an evaluation of "Techniques d'education en services de garde" (TESG) programs, or educational methods in child care services. This report presents results from the evaluation in three parts. The first section describes the following four stages of the…

  3. 600 yr High-Resolution Climate Reconstruction of the Atlantic Multidecadal Variability deduced from a Puerto Rican Speleothem

    NASA Astrophysics Data System (ADS)

    Winter, A.; Vieten, R.

    2015-12-01

    A multi-proxy speleothem study tracks the regional hydrological variability in Puerto Rico and highlights its close relation to the Atlantic Multidecadal Oscillation. Our proxy record extends instrumental observations 600 years into the past, and reveals the range of natural hydrologic variability for the region. A detailed interpretation and understanding of the speleothem climate record is achieved by the combination of multi-proxy measurements, thin section petrography, XRD analysis and cave monitoring results. The speleothem was collected in Cueva Larga, a one mile-long cave system that has been monitored since 2012. MC-ICPMS 230Th/U-dating reveals that the speleothem grew constantly over the last 600 years. Trace element ratios (Sr/Ca and Mg/Ca) as well as stable isotope ratios (δ18O and δ13C) elucidate significant changes in atmospheric precipitation at the site. Monthly cave monitoring results demonstrate that the epikarst system responds to multi-annual changes in seepage water recharge. The drip water isotope and trace element composition lack short term or seasonal variability. This hydrological system creates favorable conditions to deduce decadal climate variability from Cueva Larga's climate record. The speleothem time series mimics the most-recently published AMO reconstruction over the last 200 years with a time lag of 10-20 years. The time lag seems to results from slow atmospheric signal transmission through the epikarst but the effect of dating uncertainties cannot be ruled out. Warm SSTs in the North Atlantic are related to drier conditions in Puerto Rico. During times of decreased rainfall a relative increase in prior calcite precipitation seems to be the main process causing increased Mg/Ca trace element ratios. High trace element ratios correlate to higher δ13C values. The increase in both proxies indicates a shift towards time periods of decreased rainfall. Over the past 600 years there are two intervals of increased Mg/Ca and δ13C values

  4. Current Deformation in Central Afar and Triple Junction Kinematics Deduced from GPS and InSAR Measurements

    NASA Astrophysics Data System (ADS)

    Cécile, Doubre; Aline, Déprez; Frédéric, Masson; Anne, Socquet; Elias, Lewi; Raphael, Grandin; Alexandre, Nercessian; Patrice, Ulrich; Jean-Bernard, De Chabalier; Ibrahim, Saad; Ahmadine, Abayazid; Gilles, Peltzer; Arthur, Delorme; Eric, Calais; Tim, Wright

    2016-11-01

    Kinematics of divergent boundaries and Rift-Rift-Rift junctions are classically studied using long-term geodetic observations. Since significant magma-related displacements are expected, short-term deformation provides important constraints on the crustal mechanisms involved both in active rifting and in transfer of extensional deformation between spreading axes. Using InSAR and GPS data, we analyze the surface deformation in the whole Central Afar region in detail, focusing on both the extensional deformation across the Quaternary magmato-tectonic rift segments, and on the zones of deformation transfer between active segments and spreading axes. The largest deformation occurs across the two recently activated Asal-Ghoubbet (AG) and MH-D magmato-tectonic segments with very high strain rates, whereas the other Quaternary active segments do not concentrate any large strain, suggesting that these rifts are either sealed during inter-dyking periods or not mature enough to remain a plate boundary. Outside of these segments, the GPS horizontal velocity field shows a regular gradient following a clockwise rotation of the displacements from the Southeast to the East of Afar, with respect to Nubia. Very few shallow creeping structures can be identified as well in the InSAR data. However, using these data together with the strain rate tensor and the rotations rates deduced from GPS baselines, the present-day strain field over Central Afar is consistent with the main tectonic structures, and therefore with the long-term deformation. We investigate the current kinematics of the triple junction included in our GPS data set by building simple block models. The deformation in Central Afar can be described by adding a central micro-block evolving separately from the three surrounding plates. In this model, the northern block boundary corresponds to a deep EW-trending trans-tensional dislocation, locked from the surface to 10-13 km and joining at depth the active spreading axes of

  5. 600 yr High-Resolution Climate Reconstruction of the Atlantic Multidecadal Oscillation deduced from a Puerto Rican Speleothem

    NASA Astrophysics Data System (ADS)

    Vieten, Rolf; Winter, Amos; Scholz, Denis; Black, David; Spoetl, Christoph; Winterhalder, Sophie; Koltai, Gabriella; Schroeder-Ritzrau, Andrea; Terzer, Stefan; Zanchettin, Davide; Mangini, Augusto

    2016-04-01

    A multi-proxy speleothem study tracks the regional hydrological variability in Puerto Rico and highlights its close relation to the Atlantic Multidecadal Oscillation (AMO) describing low-frequency sea-surface temperature (SST) variability in the North Atlantic ocean. Our proxy record extends instrumental observations 600 years into the past, and reveals the range of natural hydrologic variability for the region. A detailed interpretation and understanding of the speleothem climate record is achieved by the combination of multi-proxy measurements, thin section petrography, XRD analysis and cave monitoring results. The speleothem was collected in Cueva Larga, a one mile-long cave system that has been monitored since 2012. MC-ICPMS 230Th/U-dating reveals that the speleothem grew constantly over the last 600 years. Trace element ratios (Sr/Ca and Mg/Ca) as well as stable isotope ratios (δ18O and δ13C) elucidate significant changes in atmospheric precipitation at the site. Monthly cave monitoring results demonstrate that the epikarst system responds to multi-annual changes in seepage water recharge. The drip water isotope and trace element composition lack short term or seasonal variability. This hydrological system creates favorable conditions to deduce decadal climate variability from Cueva Larga's climate record. The speleothem time series mimics the most recent AMO reconstruction over the last 200 years (Svendsen et al., 2014) with a time lag of 10-20 years. The lag seems to results from slow atmospheric signal transmission through the epikarst but the effect of dating uncertainties cannot be ruled out. Warm SSTs in the North Atlantic are related to drier conditions in Puerto Rico. During times of decreased rainfall a relative increase in prior calcite precipitation seems to be the main process causing increased Mg/Ca trace element ratios. High trace element ratios correlate to higher δ13C values. The increase in both proxies indicates a shift towards time

  6. Tables of Neutron-Induced Fission Cross Section for Various Pu, U, and Th Isotopes, Deduced from Measured Fission Probabilites

    SciTech Connect

    Younes, W; Britt, H C

    2003-03-31

    Cross sections for neutron-induced fission of {sup 231,233}Th, {sup 234,235,236,237,239}U, and {sup 240,241,243}Pu are presented in tabular form for incident neutron energies of 0.1 {le} E{sub n}(MeV) {le} 2.5. The cross sections were obtained by converting measured fission probabilities from (t,pf) reactions on mass-A targets to (n,f) cross sections on mass-A + 1 neutron targets, by using modeling to compensate for the differences in the reaction mechanisms. Data from Britt et al. were used for the {sup 234}U(t,pf) reaction, from Cramer et al. for the {sup 230,232}Th(t,pf), {sup 236,238}U(t,pf), and {sup 240,242}Pu(t,pf) reactions, and from Britt et al. for the {sup 233,235}U(t,pf) and {sup 239}Pu(t,pf) reactions. The fission probabilities P{sub (t,pf)}(E{sub x}), measured as a function of excitation energy E{sub x} of the compound system formed by the (t,p) reaction, are listed in the tables with the corresponding deduced cross sections as a function of incident neutron energy E{sub n}, {sigma}{sub (n,f)}(E{sub n}). The excitation energy and incident neutron energy are related by E{sub x} = E{sub n} + B{sub n}, where B{sub n}, where B{sub n} is the neutron binding energy. Comparison with ENDF/B-VI evaluations of the well-measured {sup 234,235,236}U(n,f) and {sup 240,241}Pu(n,f) cross sections confirms the accuracy of the present results within a 10% standard deviation above E{sub n} = 1 MeV. Below E{sub n} = 1 MeV, localized deviations of at most {+-} 20% are observed.

  7. Current deformation in Central Afar and triple junction kinematics deduced from GPS and InSAR measurements

    NASA Astrophysics Data System (ADS)

    Doubre, Cécile; Déprez, Aline; Masson, Frédéric; Socquet, Anne; Lewi, Elias; Grandin, Raphaël; Nercessian, Alexandre; Ulrich, Patrice; De Chabalier, Jean-Bernard; Saad, Ibrahim; Abayazid, Ahmadine; Peltzer, Gilles; Delorme, Arthur; Calais, Eric; Wright, Tim

    2017-02-01

    Kinematics of divergent boundaries and Rift-Rift-Rift junctions are classically studied using long-term geodetic observations. Since significant magma-related displacements are expected, short-term deformation provides important constraints on the crustal mechanisms involved both in active rifting and in transfer of extensional deformation between spreading axes. Using InSAR and GPS data, we analyse the surface deformation in the whole Central Afar region in detail, focusing on both the extensional deformation across the Quaternary magmato-tectonic rift segments, and on the zones of deformation transfer between active segments and spreading axes. The largest deformation occurs across the two recently activated Asal-Ghoubbet (AG) and Manda Hararo-Dabbahu (MH-D) magmato-tectonic segments with very high strain rates, whereas the other Quaternary active segments do not concentrate any large strain, suggesting that these rifts are either sealed during interdyking periods or not mature enough to remain a plate boundary. Outside of these segments, the GPS horizontal velocity field shows a regular gradient following a clockwise rotation of the displacements from the Southeast to the East of Afar, with respect to Nubia. Very few shallow creeping structures can be identified as well in the InSAR data. However, using these data together with the strain rate tensor and the rotations rates deduced from GPS baselines, the present-day strain field over Central Afar is consistent with the main tectonic structures, and therefore with the long-term deformation. We investigate the current kinematics of the triple junction included in our GPS data set by building simple block models. The deformation in Central Afar can be described by adding a central microblock evolving separately from the three surrounding plates. In this model, the northern block boundary corresponds to a deep EW-trending trans-tensional dislocation, locked from the surface to 10-13 km and joining at depth the

  8. SIMMAX: A modern analog technique to deduce Atlantic sea surface temperatures from planktonic foraminifera in deep-sea sediments

    NASA Astrophysics Data System (ADS)

    Pflaumann, Uwe; Duprat, Josette; Pujol, Claude; Labeyrie, Laurent D.

    1996-02-01

    leave the system.) (Paper 95PA01743,SIMMAX: A modern analog technique to deduce Atlantic sea surfacetemperatures from planktonic foraminifera in deep-sea sediments, UwePflaumann, Josette Duprat, Claude Pujol, and Laurent D. Labeyrie).Diskette may be ordered from American Geophysical Union, 2000Florida Avenue, N.W., Washington, DC 20009; Payment mustaccompany order.

  9. Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence.

    PubMed

    Yang, Zhixiu; Guo, Qiang; Goto, Simon; Chen, Yuling; Li, Ningning; Yan, Kaige; Zhang, Yixiao; Muto, Akira; Deng, Haiteng; Himeno, Hyouta; Lei, Jianlin; Gao, Ning

    2014-05-01

    The in vivo assembly of ribosomal subunits is a highly complex process, with a tight coordination between protein assembly and rRNA maturation events, such as folding and processing of rRNA precursors, as well as modifications of selected bases. In the cell, a large number of factors are required to ensure the efficiency and fidelity of subunit production. Here we characterize the immature 30S subunits accumulated in a factor-null Escherichia coli strain (∆rsgA∆rbfA). The immature 30S subunits isolated with varying salt concentrations in the buffer system show interesting differences on both protein composition and structure. Specifically, intermediates derived under the two contrasting salt conditions (high and low) likely reflect two distinctive assembly stages, the relatively early and late stages of the 3' domain assembly, respectively. Detailed structural analysis demonstrates a mechanistic coupling between the maturation of the 5' end of the 17S rRNA and the assembly of the 30S head domain, and attributes a unique role of S5 in coordinating these two events. Furthermore, our structural results likely reveal the location of the unprocessed terminal sequences of the 17S rRNA, and suggest that the maturation events of the 17S rRNA could be employed as quality control mechanisms on subunit production and protein translation.

  10. Origin of platinum-group mineral assemblages in a mantle tectonite at Unst deduced from mineral chemistry and osmium isotopes

    NASA Astrophysics Data System (ADS)

    Badanina, Inna Yu.; Lord, Richard A.; Malitch, Kreshimir N.; Meisel, Thomas C.

    2013-04-01

    assemblage is likely to reflect processes such as in-situ serpentinisation, alteration during emplacement or regional greenschist metamorphism. Whole-rock platinum-group element (PGE) concentrations give negatively sloped chondrite-normalized PGE patterns, typical of podiform chromitite, where refractory PGE (Os, Ir and Ru) prevail over less refractory PGE (Rh, Pt and Pd). The osmium isotope results identify similarly 'unradiogenic' 187Os/188Os values for 'primary' and 'secondary' PGM assemblages (with mean 187Os/188Os values of 0.12419 and 0.12464, respectively), being within uncertainty of the chromitite composition (0.1240±0.0006). This implies that the whole-rock Os isotope budget is largely controlled by laurite-dominant assemblages, supporting the conclusion that the 'secondary' PGM assemblage inherited the subchondritic osmium isotope signature of the 'primary' PGM. No evidence for other source contributions during later thermal events has been observed. The Os-isotope data provide further support for an Enstatite Chondrite Reservoir model for the convective upper mantle as defined by Walker et al. (2002) and are consistent with origin of the complex as a Caledonian ophiolite formed in a supra-subduction zone. This study was supported by Russian Foundation for Basic Research (grant 12-05-01166-a to IYuB) and the Uralian Division of Russian Academy of Sciences (project No 12-P-5-1020). References: Andrews, D.R.A., Brenan, J.M. (2002) Phase-equilibrium constraints on the magmatic origin of laurite and Os-Ir alloy. Can. Mineral. 40, 1705-1716. Walker, R.J., Prichard, H.M., Ishiwatari, A., Pimentel, M. (2002) The osmium isotopic composition of convecting upper mantle deduced from ophiolite chromites. Geochim. Cosmochim. Acta 66, 329-345.

  11. Deducing the magma chamber processes of middle Eocene volcanics, Sivas and Tokat regions; NE Turkey: Insights from clinopyroxene chemistry

    NASA Astrophysics Data System (ADS)

    Göçmengil, Gönenç; Karacık, Zekiye; Genç, Ş. Can; Prelevic, Dejan

    2016-04-01

    Middle Eocene Tokat and Sivas volcanic successions occur within the İzmir-Ankara-Erzincan suture zone. Different models are suggested for the development of the middle Eocene volcanism such as post-collisional, delamination and slab-breakoff models as well as the arc magmatism. In both areas, volcanic units cover all the basement units with a regional disconformity and comprise lavas spanning a compositional range from mainly basalt-basaltic andesite to a lesser amount trachyte. Here, we report mineral chemistry of different basaltic lavas through transect from northern continent (Tokat region, Pontides) to southern continent (Sivas region, Kırşehir block) to deduce the characteristics of the magma chamber processes which are active during the middle Eocene. Basaltic lavas include olivine bearing basalts (Ol-basalt: ± olivine + clinopyroxene + plagioclase); amphibole bearing basaltic andesite (Amp-basaltic andesite: amphibole + clinopyroxene + plagioclase ± biotite) and pyroxene bearing basaltic andesite (Px-basaltic andesite: clinopyroxene + plagioclase). Microlitic, glomeroporphyric and pilotaxitic texture are common. Clinopyroxene phenocrystals (macro ≥ 750 μm and micro ≤300 μm) are common in all three lava series which are investigated by transecting core to rim compositional profiles. They are generally augite and diopside; euhedral to subhedral in shape with oscillatory, normal and reverse zoning patterns. Also, all clinopyroxene phenocrystals are marked by moderately high Mg# (for Ol-basalt: 67-91; avg. 80; Amp-basaltic andesite: 76-83, avg: 80; Px -basaltic andesite 68-95, avg: 81). In Ol-basalt, clinopyroxene phenocrystals show normal zonation (high Mg# cores and low Mg# rims). In Amp-basaltic andesite, clinopyroxenes are generally homogenous in composition with minor variation of Mg# towards the rims. On the contrary, in Px-basaltic andesite, clinopyroxene macro phenocrystals show reverse zonation with the core with low Mg# and the rims with

  12. Global significance of a sub-Moho boundary layer (SMBL) deduced from high-resolution seismic observations

    USGS Publications Warehouse

    Fuchs, K.; Tittgemeyer, M.; Ryberg, T.; Wenzel, F.; Mooney, W.

    2002-01-01

    an on-going processes; nevertheless, the derived quantitative estimates of the SMBL properties provide important constraints for any hypothesis on scale-forming processes. Models to be tested by future numerical and field experiments are, for example, repeated subduction-convection stretching of oceanic lithosphere (marble-cake model) and schlieren formation at mid-ocean ridges. It is also proposed that the modeling of the observed blocking of Sn and Pn propagation at active plate margins offers a new tool to study the depth range of tectonics below the crust-mantle boundary. Finally, the deduced schlieren structure of the SMBL closes an important scale gap of three to four orders of magnitude between structural dimensions studied in petrological analysis of mantle samples (xenoliths or outcrop of oceanic lithosphere) and those imaged in classical seismological studies of the lithosphere.

  13. Modular design, application architecture, and usage of a self-service model for enterprise data delivery: The Duke Enterprise Data Unified Content Explorer (DEDUCE)

    PubMed Central

    Horvath, Monica M.; Rusincovitch, Shelley A.; Brinson, Stephanie; Shang, Howard C.; Evans, Steve; Ferranti, Jeffrey M.

    2015-01-01

    Purpose Data generated in the care of patients are widely used to support clinical research and quality improvement, which has hastened the development of self-service query tools. User interface design for such tools, execution of query activity, and underlying application architecture have not been widely reported, and existing tools reflect a wide heterogeneity of methods and technical frameworks. We describe the design, application architecture, and use of a self-service model for enterprise data delivery within Duke Medicine. Methods Our query platform, the Duke Enterprise Data Unified Content Explorer (DEDUCE), supports enhanced data exploration, cohort identification, and data extraction from our enterprise data warehouse (EDW) using a series of modular environments that interact with a central keystone module, Cohort Manager (CM). A data-driven application architecture is implemented through three components: an application data dictionary, the concept of “smart dimensions”, and dynamically-generated user interfaces. Results DEDUCE CM allows flexible hierarchies of EDW queries within a grid-like workspace. A cohort “join” functionality allows switching between filters based on criteria occurring within or across patient encounters. To date, 674 users have been trained and activated in DEDUCE, and logon activity shows a steady increase, with variability between months. A comparison of filter conditions and export criteria shows that these activities have different patterns of usage across subject areas. Conclusions Organizations with sophisticated EDWs may find that users benefit from development of advanced query functionality, complimentary to the user interfaces and infrastructure used in other well-published models. Driven by its EDW context, the DEDUCE application architecture was also designed to be responsive to source data and to allow modification through alterations in metadata rather than programming, allowing an agile response to source

  14. Modular design, application architecture, and usage of a self-service model for enterprise data delivery: the Duke Enterprise Data Unified Content Explorer (DEDUCE).

    PubMed

    Horvath, Monica M; Rusincovitch, Shelley A; Brinson, Stephanie; Shang, Howard C; Evans, Steve; Ferranti, Jeffrey M

    2014-12-01

    Data generated in the care of patients are widely used to support clinical research and quality improvement, which has hastened the development of self-service query tools. User interface design for such tools, execution of query activity, and underlying application architecture have not been widely reported, and existing tools reflect a wide heterogeneity of methods and technical frameworks. We describe the design, application architecture, and use of a self-service model for enterprise data delivery within Duke Medicine. Our query platform, the Duke Enterprise Data Unified Content Explorer (DEDUCE), supports enhanced data exploration, cohort identification, and data extraction from our enterprise data warehouse (EDW) using a series of modular environments that interact with a central keystone module, Cohort Manager (CM). A data-driven application architecture is implemented through three components: an application data dictionary, the concept of "smart dimensions", and dynamically-generated user interfaces. DEDUCE CM allows flexible hierarchies of EDW queries within a grid-like workspace. A cohort "join" functionality allows switching between filters based on criteria occurring within or across patient encounters. To date, 674 users have been trained and activated in DEDUCE, and logon activity shows a steady increase, with variability between months. A comparison of filter conditions and export criteria shows that these activities have different patterns of usage across subject areas. Organizations with sophisticated EDWs may find that users benefit from development of advanced query functionality, complimentary to the user interfaces and infrastructure used in other well-published models. Driven by its EDW context, the DEDUCE application architecture was also designed to be responsive to source data and to allow modification through alterations in metadata rather than programming, allowing an agile response to source system changes. Copyright © 2014 Elsevier

  15. High Precision Measurement of Resonance States in 18Ne , 30S , and 38Ca Nuclei using the (p,t) Reaction, and Reaction Rates in the αp- and rp-Processes

    NASA Astrophysics Data System (ADS)

    Adachi, T.; Berg, G. P. A.; Buthelezi, E. Z.; Carter, J.; Couder, M.; Fearick, R.; Förtsch, S. V.; Görres, J.; Kheswa, Y.; Mira, J.; Murray, S.; Neveling, R.; Papka, P.; Sideras-Haddad, E.; Smit, F. D.; Swartz, J. A.; Talwar, R.; Usman, I.; van Zyl, J. J.; Wiescher, M.; O'Brien, S.

    2011-10-01

    Thermonuclear runaway reactions in type 1 X-ray burst are trigged by the breakout from the hot CNO cycles and is subsequently driven by αp- and rp-processes. These time scales for the αp- and rp-process are determined by the associated reaction rates, which depend exponentially on the associated resonance energies. High precision (p,t) measurement were preformed at iThemba LABS to examine resonance states in 18Ne , 30S , and 38Ca nuclei using the K600 spectrometer with a dispersion matched beam. Preliminary analysis will be presented.

  16. Banned on Broadway but coming to a theater near you: the captive and rethinking the breadth of American anti-lesbian hostility in the 1920s and '30s.

    PubMed

    Hurewitz, Daniel

    2013-01-01

    While the 1927 Broadway closing of the lesbian-themed play The Captive has been treated as launching the homophobic culture that dominated mid-twentieth-century America, the show actually continued to play in cities around the country. It was regularly greeted by packed houses and critics who scoffed at the reaction in New York. The opposition that did emerge was often generated by publisher William Randolph Hearst, while leading civil libertarians fought against him. This demonstrates that the homophobic policies of the 1920s and '30s were not the product of a deeply homophobic society, but generated by smaller numbers of moralizing entreprenuers.

  17. Nucleotide sequence of Phaseolus vulgaris L. alcohol dehydrogenase encoding cDNA and three-dimensional structure prediction of the deduced protein

    PubMed Central

    Amelia, Kassim; Khor, Chin Yin; Shah, Farida Habib; Bhore, Subhash J.

    2015-01-01

    Background: Common beans (Phaseolus vulgaris L.) are widely consumed as a source of proteins and natural products. However, its yield needs to be increased. In line with the agenda of Phaseomics (an international consortium), work of expressed sequence tags (ESTs) generation from bean pods was initiated. Altogether, 5972 ESTs have been isolated. Alcohol dehydrogenase (AD) encoding gene cDNA was a noticeable transcript among the generated ESTs. This AD is an important enzyme; therefore, to understand more about it this study was undertaken. Objective: The objective of this study was to elucidate P. vulgaris L. AD (PvAD) gene cDNA sequence and to predict the three-dimensional (3D) structure of deduced protein. Materials and Methods: positive and negative strands of the PvAD cDNA clone were sequenced using M13 forward and M13 reverse primers to elucidate the nucleotide sequence. Deduced PvAD cDNA and protein sequence was analyzed for their basic features using online bioinformatics tools. Sequence comparison was carried out using bl2seq program, and tree-view program was used to construct a phylogenetic tree. The secondary structures and 3D structure of PvAD protein were predicted by using the PHYRE automatic fold recognition server. Results: The sequencing results analysis showed that PvAD cDNA is 1294 bp in length. It's open reading frame encodes for a protein that contains 371 amino acids. Deduced protein sequence analysis showed the presence of putative substrate binding, catalytic Zn binding, and NAD binding sites. Results indicate that the predicted 3D structure of PvAD protein is analogous to the experimentally determined crystal structure of s-nitrosoglutathione reductase from an Arabidopsis species. Conclusions: The 1294 bp long PvAD cDNA encodes for 371 amino acid long protein that contains conserved domains required for biological functions of AD. The predicted deduced PvAD protein's 3D structure reflects the analogy with the crystal structure of

  18. Concentrations of carbonyl sulfide and hydrogen cyanide in the free upper troposphere and lower stratosphere deduced from ATMOS/Spacelab 3 infrared solar occultation spectra

    NASA Technical Reports Server (NTRS)

    Zander, R.; Rinsland, C. P.; Russell, J. M., III; Farmer, C. B.; Norton, R. H.

    1988-01-01

    This paper presents the results on the volume mixing ratio profiles of carbonyl sulfide and hydrogen cyanide, deduced from the spectroscopic analysis of IR solar absorption spectra obtained in the occultation mode with the Atmospheric Trace Molecule Spectroscopy (ATMOS) instrument during its mission aboard Spacelab 3. A comparison of the ATMOS measurements for both northern and southern latitudes with previous field investigations at low midlatitudes shows a relatively good agreement. Southern Hemisphere volume mixing ratio profiles for both molecules were obtained for the first time, as were the profiles for the Northern Hemisphere covering the upper troposphere and the lower stratosphere simultaneously.

  19. New Life for a '30s Monument.

    ERIC Educational Resources Information Center

    Garrett, Michael

    1980-01-01

    As part of a districtwide construction and renovation program in Everett (Washington) an Art Deco auditorium was refurbished, theater support facilities were expanded into an old gymnasium, and cafeteria space was rebuilt to house studios. (MLF)

  20. Evolutionary Steps in the Emergence of Life Deduced from the Bottom-Up Approach and GADV Hypothesis (Top-Down Approach)

    PubMed Central

    Ikehara, Kenji

    2016-01-01

    It is no doubt quite difficult to solve the riddle of the origin of life. So, firstly, I would like to point out the kinds of obstacles there are in solving this riddle and how we should tackle these difficult problems, reviewing the studies that have been conducted so far. After that, I will propose that the consecutive evolutionary steps in a timeline can be rationally deduced by using a common event as a juncture, which is obtained by two counter-directional approaches: one is the bottom-up approach through which many researchers have studied the origin of life, and the other is the top-down approach, through which I established the [GADV]-protein world hypothesis or GADV hypothesis on the origin of life starting from a study on the formation of entirely new genes in extant microorganisms. Last, I will describe the probable evolutionary process from the formation of Earth to the emergence of life, which was deduced by using a common event—the establishment of the first genetic code encoding [GADV]-amino acids—as a juncture for the results obtained from the two approaches. PMID:26821048

  1. Evolutionary Steps in the Emergence of Life Deduced from the Bottom-Up Approach and GADV Hypothesis (Top-Down Approach).

    PubMed

    Ikehara, Kenji

    2016-01-26

    It is no doubt quite difficult to solve the riddle of the origin of life. So, firstly, I would like to point out the kinds of obstacles there are in solving this riddle and how we should tackle these difficult problems, reviewing the studies that have been conducted so far. After that, I will propose that the consecutive evolutionary steps in a timeline can be rationally deduced by using a common event as a juncture, which is obtained by two counter-directional approaches: one is the bottom-up approach through which many researchers have studied the origin of life, and the other is the top-down approach, through which I established the [GADV]-protein world hypothesis or GADV hypothesis on the origin of life starting from a study on the formation of entirely new genes in extant microorganisms. Last, I will describe the probable evolutionary process from the formation of Earth to the emergence of life, which was deduced by using a common event-the establishment of the first genetic code encoding [GADV]-amino acids-as a juncture for the results obtained from the two approaches.

  2. A Hypothesis: Life Initiated from Two Genes, as Deduced from the RNA World Hypothesis and the Characteristics of Life-Like Systems.

    PubMed

    Kawamura, Kunio

    2016-08-02

    RNA played a central role in the emergence of the first life-like system on primitive Earth since RNA molecules contain both genetic information and catalytic activity. However, there are several drawbacks regarding the RNA world hypothesis. Here, I briefly discuss the feasibility of the RNA world hypothesis to deduce the RNA functions that are essential for forming a life-like system. At the same time, I have conducted a conceptual analysis of the characteristics of biosystems as a useful approach to deduce a realistic life-like system in relation to the definition of life. For instance, an RNA-based life-like system should possess enough stability to resist environmental perturbations, by developing a cell-like compartment, for instance. Here, a conceptual viewpoint is summarized to provide a realistic life-like system that is compatible with the primitive Earth environment and the capabilities of RNA molecules. According to the empirical and conceptual analysis, I propose the hypothesis that the first life-like system could have initiated from only two genes.

  3. A Hypothesis: Life Initiated from Two Genes, as Deduced from the RNA World Hypothesis and the Characteristics of Life-Like Systems

    PubMed Central

    Kawamura, Kunio

    2016-01-01

    RNA played a central role in the emergence of the first life-like system on primitive Earth since RNA molecules contain both genetic information and catalytic activity. However, there are several drawbacks regarding the RNA world hypothesis. Here, I briefly discuss the feasibility of the RNA world hypothesis to deduce the RNA functions that are essential for forming a life-like system. At the same time, I have conducted a conceptual analysis of the characteristics of biosystems as a useful approach to deduce a realistic life-like system in relation to the definition of life. For instance, an RNA-based life-like system should possess enough stability to resist environmental perturbations, by developing a cell-like compartment, for instance. Here, a conceptual viewpoint is summarized to provide a realistic life-like system that is compatible with the primitive Earth environment and the capabilities of RNA molecules. According to the empirical and conceptual analysis, I propose the hypothesis that the first life-like system could have initiated from only two genes. PMID:27490571

  4. Human debrisoquine 4-hydroxylase (P450IID1): cDNA and deduced amino acid sequence and assignment of the CYP2D locus to chromosome 22.

    PubMed

    Gonzalez, F J; Vilbois, F; Hardwick, J P; McBride, O W; Nebert, D W; Gelboin, H V; Meyer, U A

    1988-02-01

    The enzyme P450db1 (db1) is responsible for the common human defect in drug oxidation known as the "debrisoquine/sparteine polymorphism." Polyclonal antibody against the rat db1 protein was used to screen a human liver lambda gt11 library for the db1 cDNA clone. A cDNA containing the full protein coding sequence was isolated; the deduced NH2-terminal sequence of this cDNA was identical to that derived from direct sequencing of the purified human db1 protein. Comparison of the human db1 with rat db1 revealed 71 and 73% similarities of nucleotides and amino acids, respectively. By use of human-rodent somatic cell hybrids the db1 gene was localized to human chromosome 22 (CYP2D locus).

  5. Genetic makeup of the Corynebacterium glutamicum LexA regulon deduced from comparative transcriptomics and in vitro DNA band shift assays.

    PubMed

    Jochmann, Nina; Kurze, Anna-Katharina; Czaja, Lisa F; Brinkrolf, Karina; Brune, Iris; Hüser, Andrea T; Hansmeier, Nicole; Pühler, Alfred; Borovok, Ilya; Tauch, Andreas

    2009-05-01

    The lexA gene of Corynebacterium glutamicum ATCC 13032 was deleted to create the mutant strain C. glutamicum NJ2114, which has an elongated cell morphology and an increased doubling time. To characterize the SOS regulon in C. glutamicum, the transcriptomes of NJ2114 and a DNA-damage-induced wild-type strain were compared with that of a wild-type control using DNA microarray hybridization. The expression data were combined with bioinformatic pattern searches for LexA binding sites, leading to the detection of 46 potential SOS boxes located upstream of differentially expressed transcription units. Binding of a hexahistidyl-tagged LexA protein to 40 double-stranded oligonucleotides containing the potential SOS boxes was demonstrated in vitro by DNA band shift assays. It turned out that LexA binds not only to SOS boxes in the promoter-operator region of upregulated genes, but also to SOS boxes detected upstream of downregulated genes. These results demonstrated that LexA controls directly the expression of at least 48 SOS genes organized in 36 transcription units. The deduced genes encode a variety of physiological functions, many of them involved in DNA repair and survival after DNA damage, but nearly half of them have hitherto unknown functions. Alignment of the LexA binding sites allowed the corynebacterial SOS box consensus sequence TcGAA(a/c)AnnTGTtCGA to be deduced. Furthermore, the common intergenic region of lexA and the differentially expressed divS-nrdR operon, encoding a cell division suppressor and a regulator of deoxyribonucleotide biosynthesis, was characterized in detail. Promoter mapping revealed differences in divS-nrdR expression during SOS response and normal growth conditions. One of the four LexA binding sites detected in the intergenic region is involved in regulating divS-nrdR transcription, whereas the other sites are apparently used for negative autoregulation of lexA expression.

  6. Deduced amino acid sequence, functional expression, and unique enzymatic properties of the form I and form II ribulose bisphosphate carboxylase/oxygenase from the chemoautotrophic bacterium Thiobacillus denitrificans.

    PubMed Central

    Hernandez, J M; Baker, S H; Lorbach, S C; Shively, J M; Tabita, F R

    1996-01-01

    The cbbL cbbS and cbbM genes of Thiobacillus denitrificans, encoding form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO), respectively, were found to complement a RubisCO-negative mutant of Rhodobacter sphaeroides to autotrophic growth. Endogenous T. denitrificans promoters were shown to function in R. sphaeroides, resulting in high levels of cbbL cbbS and cbbM expression in the R. sphaeroides host. This expression system provided high levels of both T. denitrificans enzymes, each of which was highly purified. The deduced amino acid sequence of the form I enzyme indicated that the large subunit was closely homologous to previously sequenced form I RubisCO enzymes from sulfur-oxidizing bacteria. The form I T. denitrificans enzyme possessed a very low substrate specificity factor and did not exhibit fallover, and yet this enzyme showed a poor ability to recover from incubation with ribulose 1,5-bisphosphate. The deduced amino acid sequence of the form II T. denitrificans enzyme resembled those of other form II RubisCO enzymes. The substrate specificity factor was characteristically low, and the lack of fallover and the inhibition by ribulose 1,5-bisphosphate were similar to those of form II RubisCO obtained from nonsulfur purple bacteria. Both form I and form II RubisCO from T. denitrificans possessed high KCO2 values, suggesting that this organism might suffer in environments containing low levels of dissolved CO2. These studies present the initial description of the kinetic properties of form I and form II RubisCO from a chemoautotrophic bacterium that synthesizes both types of enzyme. PMID:8550452

  7. Deduced amino acid sequence, functional expression, and unique enzymatic properties of the form I and form II ribulose bisphosphate carboxylase/oxygenase from the chemoautotrophic bacterium Thiobacillus denitrificans.

    PubMed

    Hernandez, J M; Baker, S H; Lorbach, S C; Shively, J M; Tabita, F R

    1996-01-01

    The cbbL cbbS and cbbM genes of Thiobacillus denitrificans, encoding form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO), respectively, were found to complement a RubisCO-negative mutant of Rhodobacter sphaeroides to autotrophic growth. Endogenous T. denitrificans promoters were shown to function in R. sphaeroides, resulting in high levels of cbbL cbbS and cbbM expression in the R. sphaeroides host. This expression system provided high levels of both T. denitrificans enzymes, each of which was highly purified. The deduced amino acid sequence of the form I enzyme indicated that the large subunit was closely homologous to previously sequenced form I RubisCO enzymes from sulfur-oxidizing bacteria. The form I T. denitrificans enzyme possessed a very low substrate specificity factor and did not exhibit fallover, and yet this enzyme showed a poor ability to recover from incubation with ribulose 1,5-bisphosphate. The deduced amino acid sequence of the form II T. denitrificans enzyme resembled those of other form II RubisCO enzymes. The substrate specificity factor was characteristically low, and the lack of fallover and the inhibition by ribulose 1,5-bisphosphate were similar to those of form II RubisCO obtained from nonsulfur purple bacteria. Both form I and form II RubisCO from T. denitrificans possessed high KCO2 values, suggesting that this organism might suffer in environments containing low levels of dissolved CO2. These studies present the initial description of the kinetic properties of form I and form II RubisCO from a chemoautotrophic bacterium that synthesizes both types of enzyme.

  8. Complete nucleotide sequence and deduced polypeptide sequence of a nonmuscle myosin heavy chain gene from Acanthamoeba: evidence of a hinge in the rodlike tail

    PubMed Central

    1987-01-01

    We have completely sequenced a gene encoding the heavy chain of myosin II, a nonmuscle myosin from the soil ameba Acanthamoeba castellanii. The gene spans 6 kb, is split by three small introns, and encodes a 1,509-residue heavy chain polypeptide. The positions of the three introns are largely conserved relative to characterized vertebrate and invertebrate muscle myosin genes. The deduced myosin II globular head amino acid sequence shows a high degree of similarity with the globular head sequences of the rat embryonic skeletal muscle and nematode unc 54 muscle myosins. By contrast, there is no unique way to align the deduced myosin II rod amino acid sequence with the rod sequence of these muscle myosins. Nevertheless, the periodicities of hydrophobic and charged residues in the myosin II rod sequence, which dictate the coiled-coil structure of the rod and its associations within the myosin filament, are very similar to those of the muscle myosins. We conclude that this ameba nonmuscle myosin shares with the muscle myosins of vertebrates and invertebrates an ancestral heavy chain gene. The low level of direct sequence similarity between the rod sequences of myosin II and muscle myosins probably reflects a general tolerance for residue changes in the rod domain (as long as the periodicities of hydrophobic and charged residues are largely maintained), the relative evolutionary "ages" of these myosins, and specific differences between the filament properties of myosin II and muscle myosins. Finally, sequence analysis and electron microscopy reveal the presence within the myosin II rodlike tail of a well-defined hinge region where sharp bending can occur. We speculate that this hinge may play a key role in mediating the effect of heavy chain phosphorylation on enzymatic activity. PMID:3040773

  9. New constraints on the rupture process of the 1999 August 17 Izmit earthquake deduced from estimates of stress glut rate moments

    NASA Astrophysics Data System (ADS)

    Clévédé, E.; Bouin, M.-P.; Bukchin, B.; Mostinskiy, A.; Patau, G.

    2004-12-01

    This paper illustrates the use of integral estimates given by the stress glut rate moments of total degree 2 for constraining the rupture scenario of a large earthquake in the particular case of the 1999 Izmit mainshock. We determine the integral estimates of the geometry, source duration and rupture propagation given by the stress glut rate moments of total degree 2 by inverting long-period surface wave (LPSW) amplitude spectra. Kinematic and static models of the Izmit earthquake published in the literature are quite different from one another. In order to extract the characteristic features of this event, we calculate the same integral estimates directly from those models and compare them with those deduced from our inversion. While the equivalent rupture zone and the eastward directivity are consistent among all models, the LPSW solution displays a strong unilateral character of the rupture associated with a short rupture duration that is not compatible with the solutions deduced from the published models. With the aim of understand this discrepancy, we use simple equivalent kinematic models to reproduce the integral estimates of the considered rupture processes (including ours) by adjusting a few free parameters controlling the western and eastern parts of the rupture. We show that the joint analysis of the LPSW solution and source tomographies allows us to elucidate the scattering of source processes published for this earthquake and to discriminate between the models. Our results strongly suggest that (1) there was significant moment released on the eastern segment of the activated fault system during the Izmit earthquake; (2) the apparent rupture velocity decreases on this segment.

  10. Evolution of the Storm Magnetic Field Disturbance on Earth's Surface and the Associated Ring Current as Deduced from Multiple Ground Observatories

    NASA Astrophysics Data System (ADS)

    Shen, Chao; Zeng, Gang; Li, Xinlin; Rong, Zhaojin

    2014-05-01

    Based on the continuous magnetic field measurements of the multiple ground observatories, the structure of the geomagnetic field disturbance and its temporal variations have been investigated, so as to deduce the evolution of the storm ring current. Assuming the geomagnetic field disturbance around Earth is linear in space, the gradient of the corrected H component is obtained from the multiple ground observations. It is found that, the maximum difference of the corrected H component around the Earth surface, as calculated by the gradient of the corrected H component multiplied by the Earth diameter, well represents the asymmetric index Asy-H, but is actually more sensitive to the substorm activities than the Asy-H index. The anti-direction of the gradient of the corrected H component may be regarded as pointing to the position of the maximum partial ring current. It is shown that, for the ordinary storms (Dst>-200nT) (which may be caused by CIRs, long lasting fast solar wind flows, or prolonged south IMF, etc), the maximum partial ring current is located around the duskside during the main phase and the earlier stage of the recovery phase. At the later stage of the recovery phase, the position of the maximum partial ring current keeps rotating eastward, indicating the energetic electrons may play a significant role and the main ring current carriers may be the electrons at this stage. For the severe storms (Dst<-200nT), the position of the maximum partial ring current is not so regular, and there is the evidence that the injected electrons may contribute significantly to the ring current during the main phase of supper storms. Based on physical considerations, this investigation also provides new definitions to the symmetric index Sym-H and asymmetric index Asy-H. It is made possible that, the symmetric index Sym-H and asymmetric index Asy-H may be deduced from the measurements of the geomagnetic observatories located at local but not global area on Earth.

  11. The four hexamerin genes in the honey bee: structure, molecular evolution and function deduced from expression patterns in queens, workers and drones.

    PubMed

    Martins, Juliana R; Nunes, Francis M F; Cristino, Alexandre S; Simões, Zilá L P; Bitondi, Márcia M G

    2010-03-26

    Hexamerins are hemocyanin-derived proteins that have lost the ability to bind copper ions and transport oxygen; instead, they became storage proteins. The current study aimed to broaden our knowledge on the hexamerin genes found in the honey bee genome by exploring their structural characteristics, expression profiles, evolution, and functions in the life cycle of workers, drones and queens. The hexamerin genes of the honey bee (hex 70a, hex 70b, hex 70c and hex 110) diverge considerably in structure, so that the overall amino acid identity shared among their deduced protein subunits varies from 30 to 42%. Bioinformatics search for motifs in the respective upstream control regions (UCRs) revealed six overrepresented motifs including a potential binding site for Ultraspiracle (Usp), a target of juvenile hormone (JH). The expression of these genes was induced by topical application of JH on worker larvae. The four genes are highly transcribed by the larval fat body, although with significant differences in transcript levels, but only hex 110 and hex 70a are re-induced in the adult fat body in a caste- and sex-specific fashion, workers showing the highest expression. Transcripts for hex 110, hex 70a and hex70b were detected in developing ovaries and testes, and hex 110 was highly transcribed in the ovaries of egg-laying queens. A phylogenetic analysis revealed that HEX 110 is located at the most basal position among the holometabola hexamerins, and like HEX 70a and HEX 70c, it shares potential orthology relationship with hexamerins from other hymenopteran species. Striking differences were found in the structure and developmental expression of the four hexamerin genes in the honey bee. The presence of a potential binding site for Usp in the respective 5' UCRs, and the results of experiments on JH level manipulation in vivo support the hypothesis of regulation by JH. Transcript levels and patterns in the fat body and gonads suggest that, in addition to their primary

  12. The four hexamerin genes in the honey bee: structure, molecular evolution and function deduced from expression patterns in queens, workers and drones

    PubMed Central

    2010-01-01

    Background Hexamerins are hemocyanin-derived proteins that have lost the ability to bind copper ions and transport oxygen; instead, they became storage proteins. The current study aimed to broaden our knowledge on the hexamerin genes found in the honey bee genome by exploring their structural characteristics, expression profiles, evolution, and functions in the life cycle of workers, drones and queens. Results The hexamerin genes of the honey bee (hex 70a, hex 70b, hex 70c and hex 110) diverge considerably in structure, so that the overall amino acid identity shared among their deduced protein subunits varies from 30 to 42%. Bioinformatics search for motifs in the respective upstream control regions (UCRs) revealed six overrepresented motifs including a potential binding site for Ultraspiracle (Usp), a target of juvenile hormone (JH). The expression of these genes was induced by topical application of JH on worker larvae. The four genes are highly transcribed by the larval fat body, although with significant differences in transcript levels, but only hex 110 and hex 70a are re-induced in the adult fat body in a caste- and sex-specific fashion, workers showing the highest expression. Transcripts for hex 110, hex 70a and hex70b were detected in developing ovaries and testes, and hex 110 was highly transcribed in the ovaries of egg-laying queens. A phylogenetic analysis revealed that HEX 110 is located at the most basal position among the holometabola hexamerins, and like HEX 70a and HEX 70c, it shares potential orthology relationship with hexamerins from other hymenopteran species. Conclusions Striking differences were found in the structure and developmental expression of the four hexamerin genes in the honey bee. The presence of a potential binding site for Usp in the respective 5' UCRs, and the results of experiments on JH level manipulation in vivo support the hypothesis of regulation by JH. Transcript levels and patterns in the fat body and gonads suggest that

  13. Deduced sequences of the membrane fusion and attachment proteins of canine distemper viruses isolated from dogs and wild animals in Korea.

    PubMed

    Bae, Chae-Wun; Lee, Joong-Bok; Park, Seung-Yong; Song, Chang-Seon; Lee, Nak-Hyung; Seo, Kun-Ho; Kang, Young-Sun; Park, Choi-Kyu; Choi, In-Soo

    2013-08-01

    Canine distemper virus (CDV) causes highly contagious respiratory, gastrointestinal, and neurological diseases in wild and domestic animal species. Despite a broad vaccination campaign, the disease is still a serious problem worldwide. In this study, six field CDV strains were isolated from three dogs, two raccoon dogs, and one badger in Korea. The full sequence of the genes encoding fusion (F) and hemagglutinin (H) proteins were compared with those of other CDVs including field and vaccine strains. The phylogenetic analysis for the F and H genes indicated that the two CDV strains isolated from dogs were most closely related to Chinese strains in the Asia-1 genotype. Another four strains were closely related to Japanese strains in the Asia-2 genotype. The six currently isolated strains shared 90.2-92.1% and 88.2-91.8% identities with eight commercial vaccine strains in their nucleotide and amino acid sequences of the F protein, respectively. They also showed 90.1-91.4% and 87.8-90.7% identities with the same vaccine strains in their nucleotide and deduced amino acid sequences of the H protein, respectively. Different N-linked glycosylation sites were identified in the F and H genes of the six isolates from the prototype vaccine strain Onderstepoort. Collectively, these results demonstrate that at least two different CDV genotypes currently exist in Korea. The considerable genetic differences between the vaccine strains and wild-type isolates would be a major factor of the incomplete protection of dogs from CDV infections.

  14. Assessment of volatile compound profiles and the deduced sensory significance of virgin olive oils from the progeny of Picual×Arbequina cultivars.

    PubMed

    Pérez, Ana G; de la Rosa, Raúl; Pascual, Mar; Sánchez-Ortiz, Araceli; Romero-Segura, Carmen; León, Lorenzo; Sanz, Carlos

    2016-01-08

    Volatile compounds are responsible for most of the sensory qualities of virgin olive oil and they are synthesized when enzymes and substrates come together as olive fruit is crushed during the industrial process to obtain the oil. Here we have studied the variability among the major volatile compounds in virgin olive oil prepared from the progeny of a cross of Picual and Arbequina olive cultivars (Olea europaea L.). The volatile compounds were isolated by SPME, and analyzed by HRGC-MS and HRGC-FID. Most of the volatile compounds found in the progeny's oil are produced by the enzymes in the so-called lipoxygenase pathway, and they may be clustered into different groups according to their chain length and polyunsaturated fatty acid origin (linoleic and linolenic acids). In addition, a group of compounds derived from amino acid metabolism and two terpenes also contributed significantly to the volatile fraction, some of which had significant odor values in most of the genotypes evaluated. The volatile compound content of the progeny was very varied, widely transgressing the progenitor levels, suggesting that in breeding programs it might be more effective to consider a larger number of individuals within the same cross than using different crosses with fewer individuals. Multivariate analysis allowed genotypes with particularly interesting volatile compositions to be identified and their flavor quality deduced. Copyright © 2015 Elsevier B.V. All rights reserved.

  15. Nucleotide and deduced amino acid sequences of the matrix (M) and fusion (F) protein genes of cetacean morbilliviruses isolated from a porpoise and a dolphin.

    PubMed

    Bolt, G; Blixenkrone-Møller, M; Gottschalck, E; Wishaupt, R G; Welsh, M J; Earle, J A; Rima, B K

    1994-12-01

    Morbilliviruses have been isolated from stranded dolphins and porpoises. The present paper describes the cloning and sequencing of the porpoise morbillivirus (PMV) F gene and of the dolphin morbillivirus (DMV) M and F genes and their flanking regions. The gene order of the DMV genome appeared to be identical to that of other morbilliviruses. A genomic untranslated region of 837 nucleotides was found between the translated DMV M and F gene regions. The predicted DMV M protein were highly conserved with those of other morbilliviruses. Both the deduced PMV and DMV F0 proteins exhibited three major hydrophobic regions as well as a cysteine rich region, a leucine zipper motif and a cleavage motif allowing cleavage of the F0 protein into F1 and F2 subunits. Apparently the DMV F0 cleavage motif was not modified by adaptation of DMV to Vero cells. The predicted PMV and DMV F proteins were 94% identical. Comparisons with the corresponding sequences of other morbilliviruses demonstrated that the cetacean morbillivirus does not derive from any known morbillivirus but represents an independent morbillivirus lineage.

  16. Metabolic Switches and Adaptations Deduced from the Proteomes of Streptomyces coelicolor Wild Type and phoP Mutant Grown in Batch Culture*

    PubMed Central

    Thomas, Louise; Hodgson, David A.; Wentzel, Alexander; Nieselt, Kay; Ellingsen, Trond E.; Moore, Jonathan; Morrissey, Edward R.; Legaie, Roxane; Wohlleben, Wolfgang; Rodríguez-García, Antonio; Martín, Juan F.; Burroughs, Nigel J.; Wellington, Elizabeth M. H.; Smith, Margaret C. M.

    2012-01-01

    Bacteria in the genus Streptomyces are soil-dwelling oligotrophs and important producers of secondary metabolites. Previously, we showed that global messenger RNA expression was subject to a series of metabolic and regulatory switches during the lifetime of a fermentor batch culture of Streptomyces coelicolor M145. Here we analyze the proteome from eight time points from the same fermentor culture and, because phosphate availability is an important regulator of secondary metabolite production, compare this to the proteome of a similar time course from an S. coelicolor mutant, INB201 (ΔphoP), defective in the control of phosphate utilization. The proteomes provide a detailed view of enzymes involved in central carbon and nitrogen metabolism. Trends in protein expression over the time courses were deduced from a protein abundance index, which also revealed the importance of stress pathway proteins in both cultures. As expected, the ΔphoP mutant was deficient in expression of PhoP-dependent genes, and several putatively compensatory metabolic and regulatory pathways for phosphate scavenging were detected. Notably there is a succession of switches that coordinately induce the production of enzymes for five different secondary metabolite biosynthesis pathways over the course of the batch cultures. PMID:22147733

  17. Predictable 'meta-mechanisms' emerge from feedbacks between transpiration and plant growth and cannot be simply deduced from short-term mechanisms.

    PubMed

    Tardieu, François; Parent, Boris

    2016-08-29

    Growth under water deficit is controlled by short-term mechanisms but, because of numerous feedbacks, the combination of these mechanisms over time often results in outputs that cannot be deduced from the simple inspection of individual mechanisms. It can be analysed with dynamic models in which causal relationships between variables are considered at each time-step, allowing calculation of outputs that are routed back to inputs for the next time-step and that can change the system itself. We first review physiological mechanisms involved in seven feedbacks of transpiration on plant growth, involving changes in tissue hydraulic conductance, stomatal conductance, plant architecture and underlying factors such as hormones or aquaporins. The combination of these mechanisms over time can result in non-straightforward conclusions as shown by examples of simulation outputs: 'over production of abscisic acid (ABA) can cause a lower concentration of ABA in the xylem sap ', 'decreasing root hydraulic conductance when evaporative demand is maximum can improve plant performance' and 'rapid root growth can decrease yield'. Systems of equations simulating feedbacks over numerous time-steps result in logical and reproducible emergent properties that can be viewed as 'meta-mechanisms' at plant level, which have similar roles as mechanisms at cell level.

  18. Case Study of a Hailstorm in Colorado. Part II: Particle Growth Processes at Mid-Levels Deduced from in-situ Measurements.

    NASA Astrophysics Data System (ADS)

    Heymsfield, Andrew J.; Musil, Dennis J.

    1982-12-01

    The microphysical, thermodynamic and vertical air motion characteristics of a hailstorm which occurred in northeast Colorado were investigated through penetrations by the T-28 research aircraft. On the basis of these measurements, the structure and composition of the regions of the strongest updrafts, the peripheral updraft and downdraft regions, and the quiescent regions of the storm were estimated.Several general aspects of the hydrometeor development processes operating within the storm were deduced from these measurements. The particle habits and sizes at any location within the storm depended primarily upon their position relative to the main updraft core. A region which extended around the forward portion of the storm was found to be an environment in which ice particles could grow rapidly through aggregation so that they were subsequently in a position to serve as graupel and hail embryos. Particles grew most rapidly along the boundaries of the WER, because the depletion of the liquid water by particle growth and by entrainment were lowest in these regions. Secondary ice particle production, evidently through an ice crystal collisional-breakup mechanism, is discussed.

  19. Experimental and data analysis techniques for deducing collision-induced forces from photographic histories of engine rotor fragment impact/interaction with a containment ring

    NASA Technical Reports Server (NTRS)

    Yeghiayan, R. P.; Leech, J. W.; Witmer, E. A.

    1973-01-01

    An analysis method termed TEJ-JET is described whereby measured transient elastic and inelastic deformations of an engine-rotor fragment-impacted structural ring are analyzed to deduce the transient external forces experienced by that ring as a result of fragment impact and interaction with the ring. Although the theoretical feasibility of the TEJ-JET concept was established, its practical feasibility when utilizing experimental measurements of limited precision and accuracy remains to be established. The experimental equipment and the techniques (high-speed motion photography) employed to measure the transient deformations of fragment-impacted rings are described. Sources of error and data uncertainties are identified. Techniques employed to reduce data reading uncertainties and to correct the data for optical-distortion effects are discussed. These procedures, including spatial smoothing of the deformed ring shape by Fourier series and timewise smoothing by Gram polynomials, are applied illustratively to recent measurements involving the impact of a single T58 turbine rotor blade against an aluminum containment ring. Plausible predictions of the fragment-ring impact/interaction forces are obtained by one branch of this TEJ-JET method; however, a second branch of this method, which provides an independent estimate of these forces, remains to be evaluated.

  20. Hemispheric Asymmetry in Transition from Equatorial Plasma Bubble to Blob as Deduced from 630.0 nm Airglow Observations at Low Latitudes

    NASA Technical Reports Server (NTRS)

    Park, Jaeheung; Martinis, Carlos R.; Luehr, Hermann; Pfaff, Robert F.; Kwak, Young-Sil

    2016-01-01

    Transitions from depletions to enhancements of 630.0 nm nighttime airglow have been observed at Arecibo. Numerical simulations by Krall et al. (2009) predicted that they should occur only in one hemisphere, which has not yet been confirmed observationally. In this study we investigate the hemispheric conjugacy of the depletion-to-enhancement transition using multiple instruments. We focus on one event observed in the American longitude sector on 22 December 2014: 630.0 nm airglow depletions evolved into enhancements in the Northern Hemisphere while the evolution did not occur in the conjugate location in the Southern Hemisphere. Concurrent plasma density measured by low Earth orbit (LEO) satellites and 777.4 nm airglow images support that the depletions and enhancements of 630.0 nm night time airglow reflect plasma density decreases and increases (blobs), respectively. Characteristics of the airglow depletions, in the context of the LEO satellite data, further suggest that the plasma density depletion deduced from the airglow data represents equatorial plasma bubbles (EPBs) rather than medium-scale traveling ionospheric disturbances from midlatitudes. Hence, the event in this study can be interpreted as EPB-to-blob transition.

  1. Imputation approach for deducing a complete mitogenome sequence from low-depth-coverage next-generation sequencing data: application to ancient remains from the Moon Pyramid, Mexico.

    PubMed

    Mizuno, Fuzuki; Kumagai, Masahiko; Kurosaki, Kunihiko; Hayashi, Michiko; Sugiyama, Saburo; Ueda, Shintaroh; Wang, Li

    2017-02-16

    It is considered that more than 15 depths of coverage are necessary for next-generation sequencing (NGS) data to obtain reliable complete nucleotide sequences of the mitogenome. However, it is difficult to satisfy this requirement for all nucleotide positions because of problems obtaining a uniform depth of coverage for poorly preserved materials. Thus, we propose an imputation approach that allows a complete mitogenome sequence to be deduced from low-depth-coverage NGS data. We used different types of mitogenome data files as panels for imputation: a worldwide panel comprising all the major haplogroups, a worldwide panel comprising sequences belonging to the estimated haplogroup alone, a panel comprising sequences from the population most closely related to an individual under investigation, and a panel comprising sequences belonging to the estimated haplogroup from the population most closely related to an individual under investigation. The number of missing nucleotides was drastically reduced in all the panels, but the contents obtained by imputation were quite different among the panels. The efficiency of the imputation method differed according to the panels used. The missing nucleotides were most credibly imputed using sequences of the estimated haplogroup from the population most closely related to the individual under investigation as a panel.Journal of Human Genetics advance online publication, 16 February 2017; doi:10.1038/jhg.2017.14.

  2. Experimental model proposed to deduce pharmacological evidence of the beta-cell neogenesis activity of Argyrolobium roseum vis-a-vis an evaluation of its hypoglycemic activity

    PubMed Central

    Gupta, OP; Malhotra, Pavan; Kudyar, Surbhi; Singh, Aneeta; Gupta, Geetika

    2011-01-01

    Background: Argyrolobium roseum is described in texts for its botanical aspects with no mention of its therapeutic uses. Recently, a solvent fraction of this plant has been reported to possess insulin secretagogue activity. Objective: Primary objective of the study was to evaluate the hypoglycemic activity of different fractions of Argyrolobium roseum plant. Secondary objective was to devise a model for detecting its beta-cell neogenesis activity. Materials and Methods: Alcoholic fraction (AR) was studied in fasting, glucose-loaded and streptozotocin (STZ)-treated hyperglycemic rats. For deducing beta-cell neogenesis activity, a sub-lethal dose of STZ, causing delayed death in the animals, was administered. Results: AR in a dose of 200 mg/kg showed hypoglycemic activity in fasting and STZ-treated rats. With sub-lethal dose of STZ, recovery from hyperglycemia was seen. Conclusion: AR of Argyrolobium roseum possesses hypoglycemic and a possible beta-cell neogenesis activity. Further studies are required to confirm its beta-cell neogenesis potential by cytological methods and to establish its role in the treatment of type 2 diabetes. PMID:23776786

  3. Structure of LEP100, a glycoprotein that shuttles between lysosomes and the plasma membrane, deduced from the nucleotide sequence of the encoding cDNA

    PubMed Central

    1988-01-01

    LEP100, a membrane glycoprotein that has the unique property of shuttling from lysosomes to endosomes to plasma membrane and back, was purified from chicken brain. Its NH2-terminal amino acid sequence was determined, and an oligonucleotide encoding part of this sequence was used to clone the encoding cDNA. The deduced amino acid sequence consists of 414 residues of which the NH2-terminal 18 constitute a signal peptide. The sequence includes 17 sites for N-glycosylation in the NH2-terminal 75% of the polypeptide chain followed by a region lacking N-linked oligosaccharides, a single possible membrane-spanning segment, and a cytoplasmic domain of 11 residues, including three potential phosphorylation sites. Eight cysteine residues are spaced in a regular pattern through the lumenal (extracellular) domain, while a 32-residue sequence rich in proline, serine, and threonine occurs at its midpoint. Expression of the cDNA in mouse L cells resulted in targeting of LEP100 primarily to the mouse lysosomes. PMID:3339090

  4. Hemispheric asymmetry in transition from equatorial plasma bubble to blob as deduced from 630.0 nm airglow observations at low latitudes

    NASA Astrophysics Data System (ADS)

    Park, Jaeheung; Martinis, Carlos R.; Lühr, Hermann; Pfaff, Robert F.; Kwak, Young-Sil

    2016-01-01

    Transitions from depletions to enhancements of 630.0 nm nighttime airglow have been observed at Arecibo. Numerical simulations by Krall et al. (2009) predicted that they should occur only in one hemisphere, which has not yet been confirmed observationally. In this study we investigate the hemispheric conjugacy of the depletion-to-enhancement transition using multiple instruments. We focus on one event observed in the American longitude sector on 22 December 2014: 630.0 nm airglow depletions evolved into enhancements in the Northern Hemisphere while the evolution did not occur in the conjugate location in the Southern Hemisphere. Concurrent plasma density measured by low Earth orbit (LEO) satellites and 777.4 nm airglow images support that the depletions and enhancements of 630.0 nm nighttime airglow reflect plasma density decreases and increases (blobs), respectively. Characteristics of the airglow depletions, in the context of the LEO satellite data, further suggest that the plasma density depletion deduced from the airglow data represents equatorial plasma bubbles (EPBs) rather than medium-scale traveling ionospheric disturbances from midlatitudes. Hence, the event in this study can be interpreted as EPB-to-blob transition.

  5. Structural and dynamic aspects of binding of prototype lexitropsin to the decadeoxyribonucleotide d(CGCAATTGCC)/sub 2/ deduced from high-resolution /sup 1/H NMR studies

    SciTech Connect

    Lee, M.; Chang, D.K.; Hartley, J.A.; Pon, R.T.; Krowicki, K.; Lown, J.W.

    1988-01-12

    Structural and dynamic properties of the self-complementary decadeoxyribonucleotide d-(CGCAATTGCC)/sub 2/ and the interaction between a prototype lexitropsin, or information-reading oligopeptide, and the decadeoxyribonucleotide and are deduced by using high-resolution /sup 1/H NMR techniques. The nonexchangeable and imino proton resonances of d(CGCAATTGCG)/sub 2/ have been completely assigned by two-dimensional NMR studies. The decadeoxyribonucleotide exists as a right-handed B-DNA. In the /sup 1/H NMR spectrum of the l:1 complex, the selective chemical shifts and removal of degeneracy of AH2(4), AH2(5), T-CH/sub 3/(6), and T-CH/sub 3/(7) due to the anisotropy effects of the heterocyclic moieties of the ligand, and with lesser effects at the flanking base sites C(3) and G(8), locate the drug centrally in the decadeoxyribonucleotide. This conclusion is supported by plots of individual chemical shift changes across the decadeoxyribonucleotide. Strong nuclear Overhauser effects (NOEs) between pyrrol H5 and AH2(5), and weaker NOEs to AH1'(5), TH3'(6), and AH2'(5), firmly locate the ligand in the minor groove. Intraligand NOEs between the adjacent heterocyclic moieties indicate that the lexitropsin is subject to propeller twisting about the N6-C9 bond in both the bound and free forms. Nuclear Overhauser effect spectroscopy (NOESY) and correlated spectroscopy (COSY) experiments also indicate that the removal of degeneracy of the C16 methylene protons upon complexation may arise from restricted rotation about the C15-N9, C15-C16, and C16-C17 bonds. NOE measurements on the decadeoxyribonucleotide in the 1:1 complex confirm it exists as a right-handed helix and belongs to the B family. Alternative mechanisms for this exchange process are considered.

  6. Deducing the molecular properties of zwitterionic, protonated, deprotonated, and double-deprotonated forms of L-cysteine from vibrational spectroscopy (IR, Raman, VCD) and quantum chemical calculations.

    PubMed

    Quesada-Moreno, María Mar; Avilés-Moreno, Juan Ramón; Márquez-García, A A; López-González, Juan Jesús

    2014-06-01

    The behavior of L-cysteine (C3H7NO2S, (2R)-2-amino-3-sulfanylpropanoic acid) in water at different pH values was analyzed both experimentally and theoretically. The behavior was studied at pH values of 5.21 (at this pH, L-cysteine is a zwitterionic species), 1.00 (protonated species), 8.84 (monodeprotonated species), and 13.00 (dideprotonated species). We carried out a vibrational study using nonchiroptical (IR-Raman) and chiroptical (VCD) techniques complemented by quantum chemical calculations. We adopted a dual strategy, as follows. (i) The hybrid density functionals B3LYP and M062X and the ab initio MP2 method were employed, with the same 6-311++G (d,p) basis set, in order to characterize the relative energies and structures of an extensive set of conformers of L-cysteine. The presence of water was included by utilizing the IEF-PCM implicit solvation model. (ii) The vibrational analysis was made using a chirality-sensitive using a chirality-sensitive technique (VCD) and chirality-insensitive techniques (IR, including MIR and FIR, and Raman), especially in aqueous solution. The results obtained theoretically and experimentally were compared in order to deduce the most stable structures at each pH. Moreover, for the first time, the monodeprotonated anion of L-cysteine was detected in aqueous solution by means of IR, Raman and vibrational circular dichroism (VCD). Finally, analysis of the low-frequency region using the IR and Raman techniques was shown to be a very important way to understanding the conformational preference of the zwitterionic species.

  7. Upper Mantle Seismic Anisotropy Patterns around the La Réunion Hotspot deduced from SKS-splitting measurements: Plate, Plume and Ridges signatures

    NASA Astrophysics Data System (ADS)

    Scholz, John-Robert; Barruol, Guilhem; Fontaine, Fabrice R.; Mazzullo, Alessandro; Montagner, Jean-Paul; Stutzmann, Eléonore; Sigloch, Karin

    2017-04-01

    We present results of upper mantle seismic anisotropy in the Southwest Indian Ocean around the hotspot of La Réunion, deduced from SKS splitting measurements using the 'SplitLab' toolbox. Data analysed in this study were recorded by 20 terrestrial and 57 ocean-bottom three-component seismometers installed in the framework of the RHUM-RUM project (www.rhum-rum.net). Broad-band and wide-band ocean-bottom instruments were deployed around the La Réunion Island and along the Central and Southwest Indian Ridges (deployment: R/V Marion Dufresne, 2012, MD192 - recovery: R/V Meteor, 2013, M101), and recorded for 8 to 13 months. We discuss the anisotropy signatures that are potentially induced by the absolute motion of the African Plate, by the spreading of the Central and Southwest Indian Mid-Ocean Ridges (CIR & SWIR), and by the interaction of the ascending plume with the overlying lithosphere and the neighbouring CIR and SWIR. The observed pattern displays a ridge-parallel anisotropy beneath the SWIR that suggests an along-axis upper mantle flow controlled by the thick and cold lithosphere on both sides of the ridge. We furthermore observe a coherent regional anisotropy pattern between La Réunion and the CIR. Both body and surface wave analysis suggest that this dominant flow is located at asthenospheric depths and could be consistent with a preserved feeding of the ridge by the mantle upwelling associated with the Réunion hotspot, as first proposed by Morgan (1978). Finally, we quantitatively compare the azimuthal anisotropy derived from SKS splitting with those from surface wave data.

  8. Nucleotide and deduced amino acid sequences of a subtilisin-like serine protease from a deep-sea bacterium, Alkalimonas collagenimarina AC40(T).

    PubMed

    Kurata, Atsushi; Uchimura, Kohsuke; Shimamura, Shigeru; Kobayashi, Tohru; Horikoshi, Koki

    2007-11-01

    The acpI gene encoding an alkaline protease (AcpI) from a deep-sea bacterium, Alkalimonas collagenimarina AC40(T), was shotgun-cloned and sequenced. It had a 1,617-bp open reading frame encoding a protein of 538 amino acids. Based on analysis of the deduced amino acid sequence, AcpI is a subtilisin-like serine protease belonging to subtilase family A. It consists of a prepropeptide, a catalytic domain, and a prepeptidase C-terminal domain like other serine proteases from the genera Pseudomonas, Shewanella, Alteromonas, and Xanthomonas. Heterologous expression of the acpI gene in Escherichia coli cells yielded a 28-kDa recombinant AcpI (rAcpI), suggesting that both the prepropeptide and prepeptidase C-terminal domains were cleaved off to give the mature form. Analysis of N-terminal and C-terminal amino acid sequences of purified rAcpI showed that the mature enzyme would be composed of 273 amino acids. The optimal pH and temperature for the caseinolytic activity of the purified rAcpI were 9.0-9.5 and 45 degrees C in 100 mM glycine-NaOH buffer. Calcium ions slightly enhanced the enzyme activity and stability. The enzyme favorably hydrolyzed gelatin, collagen, and casein. AcpI from A. collagenimarina AC40(T) was also purified from culture broth, and its molecular mass was around 28 kDa, indicating that the cleavage manner of the enzyme is similar to that in E. coli cells.

  9. How to deduce and teach the logical and unambiguous answer, namely L = ∑C, to “What is Life?” using the principles of communication?

    PubMed Central

    De Loof, Arnold

    2015-01-01

    Is it possible to understand the very nature of ‘Life’ and ‘Death’ based on contemporary biology? The usual spontaneous reaction is: “No way. Life is far too complicated. It involves both material- and an immaterial dimensions, and this combination exceeds the capacities of the human brain.” In this paper, a fully contrarian stand is taken. Indeed it will be shown that without invoking any unknown principle(s) unambiguous definitions can be logically deduced. The key? First ask the right questions. Next, thoroughly imbue contemporary biology with the principles of communication, including both its ‘hardware’ and its ‘software’ aspects. An integrative yet simple principle emerges saying that: 1. All living matter is invariably organized as sender-receiver compartments that incessantly handle and transfer information (= communicate); 2. The ‘communicating compartment’ is better suited to serve as universal unit of structure, function and evolution than ‘the (prokaryotic) cell’, the smallest such unit; 3. ‘Living matter’ versus ‘non-living’ are false opposites while ‘still alive’ and ‘just not alive anymore’ are true opposites; 4. ‘Death’ ensues when a given sender-receiver compartment irreversibly loses its ability to handle information at its highest level of compartmental organization; 5. The verb ‘Life’ (L) denotes nothing else than the total sum (∑) of all acts of communication (C) executed by a sender-receiver at all its levels of compartmental organization: L = ∑C; 6. Any act of communication is a problem-solving act; 6. Any Extended Evolutionary Synthesis (EES) should have the definition of Life at its core. PMID:27064373

  10. Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA

    PubMed Central

    1989-01-01

    While a number of different gap junction proteins have now been identified, hepatic gap junctions are unique in being the first demonstrated case where two homologous, but distinct, proteins (28,000 and 21,000 Mr) are found within a single gap junctional plaque (Nicholson, B. J., R. Dermietzel, D. Teplow, O. Traub, K. Willecke, and J.-P. Revel. 1987. Nature [Lond.]. 329:732-734). The cDNA for the major 28,000-Mr component has been cloned (Paul, D. L. 1986. J. Cell Biol. 103:123-134) (Kumar, N. M., and N. B. Gilula. 1986. J. Cell Biol. 103:767-776) and, based on its deduced formula weight of 32,007, has been designated connexin 32 (or Cx32 as used here). We now report the selection and characterization of clones for the second 21,000-Mr protein using an oligonucleotide derived from the amino-terminal protein sequence. Together the cDNAs represent 2.4 kb of the single 2.5- kb message detected in Northern blots. An open reading frame of 678 bp coding for a protein with a calculated molecular mass of 26,453 D was identified. Overall sequence homology with Cx32 and Cx43 (64 and 51% amino acid identities, respectively) and a similar predicted tertiary structure confirm that this protein forms part of the connexin family and is consequently referred to as Cx26. Consistent with observations on Cx43 (Beyer, E. C., D. L. Paul, and D. A. Goodenough. 1987. J. Cell Biol. 105:2621-2629) the most marked divergence between Cx26 and other members of the family lies in the sequence of the cytoplasmic domains. The Cx26 gene is present as a single copy per haploid genome in rat and, based on Southern blots, appears to contain at least one intron outside the open reading frame. Northern blots indicate that Cx32 and Cx26 are typically coexpressed, messages for both having been identified in liver, kidney, intestine, lung, spleen, stomach, testes, and brain, but not heart and adult skeletal muscle. This raises the interesting prospect of having differential modes of regulating

  11. Deducing corticotropin-releasing hormone receptor type 1 signaling networks from gene expression data by usage of genetic algorithms and graphical Gaussian models.

    PubMed

    Trümbach, Dietrich; Graf, Cornelia; Pütz, Benno; Kühne, Claudia; Panhuysen, Marcus; Weber, Peter; Holsboer, Florian; Wurst, Wolfgang; Welzl, Gerhard; Deussing, Jan M

    2010-11-19

    Dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis is a hallmark of complex and multifactorial psychiatric diseases such as anxiety and mood disorders. About 50-60% of patients with major depression show HPA axis dysfunction, i.e. hyperactivity and impaired negative feedback regulation. The neuropeptide corticotropin-releasing hormone (CRH) and its receptor type 1 (CRHR1) are key regulators of this neuroendocrine stress axis. Therefore, we analyzed CRH/CRHR1-dependent gene expression data obtained from the pituitary corticotrope cell line AtT-20, a well-established in vitro model for CRHR1-mediated signal transduction. To extract significantly regulated genes from a genome-wide microarray data set and to deduce underlying CRHR1-dependent signaling networks, we combined supervised and unsupervised algorithms. We present an efficient variable selection strategy by consecutively applying univariate as well as multivariate methods followed by graphical models. First, feature preselection was used to exclude genes not differentially regulated over time from the dataset. For multivariate variable selection a maximum likelihood (MLHD) discriminant function within GALGO, an R package based on a genetic algorithm (GA), was chosen. The topmost genes representing major nodes in the expression network were ranked to find highly separating candidate genes. By using groups of five genes (chromosome size) in the discriminant function and repeating the genetic algorithm separately four times we found eleven genes occurring at least in three of the top ranked result lists of the four repetitions. In addition, we compared the results of GA/MLHD with the alternative optimization algorithms greedy selection and simulated annealing as well as with the state-of-the-art method random forest. In every case we obtained a clear overlap of the selected genes independently confirming the results of MLHD in combination with a genetic algorithm. With two unsupervised algorithms

  12. Impact delivery of organic matter on the acapulcoite-lodranite parent-body deduced from C, N isotopes and nanostructures of carbon phases in Acapulco and Lodran

    NASA Astrophysics Data System (ADS)

    Charon, E.; Aléon, J.; Rouzaud, J.-N.

    2014-10-01

    Lodran. Carbon phases in Lodran would have been formed by the secondary carbonization of hydrocarbon fluids released during the primary carbonization of IOM. In the framework of this model, the C isotopic compositions can be reproduced using Rayleigh distillation at each carbonization step and the N isotopic compositions can be understood as resulting from the variable loss and preservation of 15N-rich nitriles (δ15N ∼ +800‰) and 15N-poor pyrroles (δ15N = -140‰) during carbonization. The combined interpretation of the temperatures deduced from this model, petrographic cooling rates, and thermochronological indicators suggest that the CI-CM IOM could have been introduced in the parent-body by an impact, about 10 Myr after solar system formation.

  13. Pu236(n,f), Pu237(n,f), and Pu238(n,f) cross sections deduced from (p,t), (p,d), and (p,p') surrogate reactions

    NASA Astrophysics Data System (ADS)

    Hughes, R. O.; Beausang, C. W.; Ross, T. J.; Burke, J. T.; Casperson, R. J.; Cooper, N.; Escher, J. E.; Gell, K.; Good, E.; Humby, P.; McCleskey, M.; Saastimoinen, A.; Tarlow, T. D.; Thompson, I. J.

    2014-07-01

    The Pu236(n,f), Pu237(n,f) and Pu238(n,f) cross sections have been inferred by utilizing the surrogate ratio method. Targets of Pu239 and U235 were bombarded with 28.5-MeV protons, and the light ion recoils, as well as fission fragments, were detected using the STARS detector array at the K150 Cyclotron at the Texas A&M cyclotron facility. The (p, tf) reaction on Pu239 and U235 targets was used to deduce the σ (Pu236(n ,f))/σ(U232(n,f)) ratio, and the Pu236(n,f) cross section was subsequently determined for En=0.5-7.5 MeV. Similarly, the (p,df) reaction on the same two targets was used to deduce the σ(Pu237(n ,f))/σ(U233(n,f)) ratio, and the Pu237(n,f) cross section was extracted in the energy range En=0.5-7 MeV. The Pu238(n,f) cross section was also deduced by utilizing the (p,p') reaction channel on the same targets. There is good agreement with the recent ENDF/B-VII.1 evaluated cross section data for Pu238(n,f) in the range En=0.5-10.5 MeV and for Pu237(n,f) in the range En=0.5-7 MeV; however, the Pu236(n,f) cross section deduced in the present work is higher than the evaluation between 2 and 7 MeV.

  14. On a Riesz basis of exponentials related to the eigenvalues of an analytic operator and application to a non-selfadjoint problem deduced from a perturbation method for sound radiation

    NASA Astrophysics Data System (ADS)

    Ellouz, Hanen; Feki, Ines; Jeribi, Aref

    2013-11-01

    In the present paper, we prove that the family of exponentials associated to the eigenvalues of the perturbed operator T(ɛ) ≔ T0 + ɛT1 + ɛ2T2 + … + ɛkTk + … forms a Riesz basis in L2(0, T), T > 0, where \\varepsilon in {C}, T0 is a closed densely defined linear operator on a separable Hilbert space H with domain D(T_0) having isolated eigenvalues with multiplicity one, while T1, T2, … are linear operators on H having the same domain Dsupset D(T_0) and satisfying a specific growing inequality. After that, we generalize this result using a H-Lipschitz function. As application, we consider a non-selfadjoint problem deduced from a perturbation method for sound radiation.

  15. On a Riesz basis of exponentials related to the eigenvalues of an analytic operator and application to a non-selfadjoint problem deduced from a perturbation method for sound radiation

    SciTech Connect

    Ellouz, Hanen; Feki, Ines; Jeribi, Aref

    2013-11-15

    In the present paper, we prove that the family of exponentials associated to the eigenvalues of the perturbed operator T(ε) ≔ T{sub 0} + εT{sub 1} + ε{sup 2}T{sub 2} + … + ε{sup k}T{sub k} + … forms a Riesz basis in L{sup 2}(0, T), T > 0, where ε∈C, T{sub 0} is a closed densely defined linear operator on a separable Hilbert space H with domain D(T{sub 0}) having isolated eigenvalues with multiplicity one, while T{sub 1}, T{sub 2}, … are linear operators on H having the same domain D⊃D(T{sub 0}) and satisfying a specific growing inequality. After that, we generalize this result using a H-Lipschitz function. As application, we consider a non-selfadjoint problem deduced from a perturbation method for sound radiation.

  16. On a Riesz basis of exponentials related to the eigenvalues of an analytic operator and application to a non-selfadjoint problem deduced from a perturbation method for sound radiation

    SciTech Connect

    Ellouz, Hanen; Feki, Ines; Jeribi, Aref

    2013-11-15

    In the present paper, we prove that the family of exponentials associated to the eigenvalues of the perturbed operator T(ε) ≔ T{sub 0} + εT{sub 1} + ε{sup 2}T{sub 2} + … + ε{sup k}T{sub k} + … forms a Riesz basis in L{sup 2}(0, T), T > 0, where ε∈C, T{sub 0} is a closed densely defined linear operator on a separable Hilbert space H with domain D(T{sub 0}) having isolated eigenvalues with multiplicity one, while T{sub 1}, T{sub 2}, … are linear operators on H having the same domain D⊃D(T{sub 0}) and satisfying a specific growing inequality. After that, we generalize this result using a H-Lipschitz function. As application, we consider a non-selfadjoint problem deduced from a perturbation method for sound radiation.

  17. Assessment of penetrating thermal tissue damage/spread associated with PhotonBlade™, Valleylab™ Pencil, Valleylab™ EDGE™ Coated Pencil, PlasmaBlade® 3.0S and PlasmaBlade® 4.0 for intraoperative tissue dissection using the fresh extirpated porcine muscle model

    NASA Astrophysics Data System (ADS)

    Bennett, Haydon E.; Taylor, Scott D.; Fugett, James H.; Shrout, Joshua L.; Davison, Paul O.; Ryan, S. Eric; Coad, James E.

    2017-02-01

    Penetrating thermal tissue damage/spread is an important aspect of many electrosurgical devices and correlates with effective tissue cutting, hemostasis, preservation of adjacent critical structures and tissue healing. This study compared the thermal damage/spread associated with the PhotonBlade, Valleylab Pencil, Valleylab EDGE Coated Pencil, PlasmaBlade 3.0S and PlasmaBlade 4.0, when performing a single pass dynamic tissue cut in fresh extirpated porcine longissimus muscle. These devices were used in a fashion that emulated their use in the clinical setting. Each device's thermal damage/spread, at Minimum, Median and Maximum power input settings, was assessed with nitroblue tetrazolium viability staining in the WVU Pathology Laboratory for Translational Medicine. The thermal damage/spread associated with the PhotonBlade was compared with the other devices tested based on the individual treatment results (n=179 cuts combined). In summary, the PhotonBlade overall demonstrated the least penetrating thermal tissue damage/spread, followed by the PlasmaBlade 4.0, then Valleylab Pencil and PlasmaBlade 3.0S and then Valleylab EDGE Coated Pencil in order of increasing thermal damage/spread depths.

  18. Deducing dust emission mechanisms from field measurements

    USDA-ARS?s Scientific Manuscript database

    Field observations are needed to both develop and test theories on dust emission for use in global modeling systems. The mechanism of dust emission (aerodynamic entrainment, saltation bombardment, aggregate disintegration) and the amount and particle-size distribution of emitted dust may vary under ...

  19. Deducing behaviors from primitive movement attributes

    NASA Astrophysics Data System (ADS)

    Liu, Danzhou; Hughes, Charles E.

    2005-03-01

    The research reported here anticipates the future of smart buildings by developing algorithms that categorize the movements of individuals based on such characteristics as motion vectors, velocity vectors, head orientation vectors and predetermined positions. The intended applications include detecting intrusions, helping lost visitors, and changing the artwork on virtual posters to reflect an individual's presumed interests. The vectors we capture represent trajectories in a multi-dimensional space. To make sense out of these, we first segment a trajectory into sub-trajectories, typically based on time. To describe each sub-trajectory, we use primitive patterns of body movement and additional information, e.g., average speed during this interval, head movement and place or object nearby. That is, for each sub-trajectory, we use a tuple of the following form: (interval_ID, body_movement, avg_speed, head_movement, places_passed). Since trajectories may have many outliers introduced by sensor failures or uneven human movement, we have developed a neural network-based pattern extraction subsystem that can handle intervals with noisy data. The choice of these attributes and our current classification of behaviors do not imply that these are the only or best ways to categorize behaviors. However, we do not see that as the focus of the research reported here. Rather, our goal is to show that the use of primitive attributes (low level), neural networks to identify categories of recognizable simple behaviors (middle level) and a regular expression-based means of describing intent (high level) is sufficient to provide a means to convert observable low-level attributes into the recognition of potential intents.

  20. Deducing a mechanism of all musculoskeletal injuries

    PubMed Central

    Verrall, Geoffrey; Dolman, Bronwyn

    2016-01-01

    Summary Background The mechanism of musculoskeletal (MSK) injuries is not well understood. This research applies principles of elastic motion to the anatomy and movement patterns of MSK structures. From this an insight into the application and timing of forces on MSK structures can be established and the mechanism/s of injury derived. Methods (Current Knowledge) All MSK structures demonstrate varying degrees of elasticity. Movement occurs primarily as a consequence of Muscle Tendon Unit (MTU) shortening. The application of an applied external force results in MSK structure lengthening. Results The MTU acts as a non-idealised Hookean Spring. The resting length of MSK structures is the minimum distance between attachment points. The anatomical constraints results in MSK structures having adequate compressive strength during shortening. Thus MSK injuries only occur during lengthening of the MSK structure. From this with knowledge of MSK movement cycles, we can derive the mechanism of injury. Conclusions MSK injuries result from an inability to counter applied forces whilst lengthening. Muscles, tendons and ligaments can only injure during their lengthening contraction phase. Insertional tendons and bone near attachment points injure during the MTU shortening phase. Injuries to other MSK structures can occur independent of the lengthening and shortening phases such as direct contact injuries. PMID:27900289

  1. Long-term changes in UT/LS ozone between the late 1970s and the 1990s deduced from the GASP and MOZAIC aircraft programs and from ozonesondes

    NASA Astrophysics Data System (ADS)

    Schnadt Poberaj, C.; Staehelin, J.; Brunner, D.; Thouret, V.; de Backer, H.; Stübi, R.

    2009-01-01

    We present ozone measurements of the Global Atmospheric Sampling Program (GASP) performed from four commercial and one research aircraft in the late 1970s to compare them with respective measurements of the ongoing MOZAIC project. Climatologies of UT/LS ozone were built using the aircraft data sets (1975-1979 and 1994-2001), and long-term changes between the 1970s and 1990s were derived by comparison. The data were binned relative to the dynamical tropopause to separate between UT and LS air masses. LS data were analysed using equivalent latitudes. In the UT, pronounced increases are found over the Middle East and South Asia in the spring and summer seasons. Increases are also found over Japan, Europe, and the eastern parts of the Unites States depending on season. LS ozone over northern mid- and high latitudes was found to be lower in the 1990s compared to the 1970s in all seasons of the year. In addition, a comparison with long-term changes deduced from ozonesondes is presented. An altitude offset was applied to the sonde data to account for the slow response time of the ozone sensors. The early 1970s European Brewer-Mast (BM) sonde data agree with GASP within the range of uncertainty (UT) or measured slightly less ozone (LS). In contrast, the 1990s BM sensors show consistently and significantly higher UT/LS ozone values than MOZAIC. This unequal behaviour of aircraft/sonde comparisons in the 1970s and 1990s leads to differences in the estimated long-term changes over Europe: while the comparison between GASP and MOZAIC indicates ozone changes of -5% to 10% over Europe, the sondes suggest a much larger increase of 10%-35% depending on station and season, although statistical significance is not conclusive due to data sample limitations. In contrast to the BM sondes, the Electrochemical Cell (ECC) sonde at Wallops Island, USA, measured higher UT ozone than both GASP and MOZAIC. Hence, long-term changes from GASP/MOZAIC agree within the range of uncertainty with

  2. Long-term changes in UT/LS ozone between the late 1970s and the 1990s deduced from the GASP and MOZAIC aircraft programs and from ozonesondes

    NASA Astrophysics Data System (ADS)

    Schnadt Poberaj, C.; Staehelin, J.; Brunner, D.; Thouret, V.; de Backer, H.; Stübi, R.

    2009-07-01

    We present ozone measurements of the Global Atmospheric Sampling Program (GASP) performed from four commercial and one research aircraft in the late 1970s to compare them with respective measurements of the ongoing MOZAIC project. Multi-annual averages of UT/LS ozone were built using the aircraft data sets (1975-1979 and 1994-2001), and long-term changes between the 1970s and 1990s were derived by comparison. The data were binned relative to the dynamical tropopause to separate between UT and LS air masses. LS data were analysed using equivalent latitudes. In the UT, pronounced increases of 20-40% are found over the Middle East and South Asia in the spring and summer seasons. Increases are also found over Japan, Europe, and the eastern parts of the United States depending on season. LS ozone over northern mid- and high latitudes was found to be lower in the 1990s compared to the 1970s in all seasons of the year. In addition, a comparison with long-term changes deduced from ozonesondes is presented. The early 1970s European Brewer-Mast (BM) sonde data agree with GASP within the range of uncertainty (UT) or measured slightly less ozone (LS). In contrast, the 1990s BM sensors show consistently and significantly higher UT/LS ozone values than MOZAIC. This unequal behaviour of aircraft/sonde comparisons in the 1970s and 1990s leads to differences in the estimated long-term changes over Europe: while the comparison between GASP and MOZAIC indicates ozone changes of -5% to 10% over Europe, the sondes suggest a much larger increase of 10%-35% depending on station and season, although statistical significance is not conclusive due to data sample limitations. In contrast to the BM sondes, the Electrochemical Cell (ECC) sonde at Wallops Island, USA, measured higher UT ozone than both GASP and MOZAIC. Hence, long-term changes from GASP/MOZAIC agree within the range of uncertainty with the changes deduced from Wallops Island.

  3. Mass Measurements for the rp Process

    NASA Astrophysics Data System (ADS)

    Kankainen, Anu; Canete, Laetitia; Eronen, Tommi; Gorelov, Dmitry; Hakala, Jani; Jokinen, Ari; Kolhinen, Veli S.; Koponen, Jukka; Moore, Iain D.; Nesterenko, Dimitrii; Reinikainen, Juuso; Rinta-Antila, Sami; Äystö, Juha

    One of the key parameters for the reaction network calculations for the rapid proton capture (rp) process, occurring e.g., in type I X-ray bursts, are the masses of the involved nuclei. Nowadays, masses of even rather exotic nuclei can be measured very precisely employing Penning-trap mass spectrometry. With the JYFLTRAP Penning trap at the IGISOL facility, masses of around 100 neutron-deficient nuclei have been determined with a typical precision of a few keV. Most recently, 25Al, 30P, 31Cl, and 52Co have been measured. Of these, the precision of the mass-excess value of 31Cl was improved from 50 to 3.4 keV, and the mass of 52Co was experimentally determined for the first time. The mass of 31Cl is relevant for estimating the waiting-point conditions for 30S as the 31Cl(γ, p)30S-30S(p, γ)31Cl equilibrium ratio depends exponentially on the Q value. For 52Co, located at the path towards 56Ni, a deviation from the extrapolated mass value has been revealed. In this contribution, recent JYFLTRAP experiments for the rp process will be discussed.

  4. L'evaluation des programmes d'etudes au Quebec. Etude de cas: Les programmes des techniques d'education en services de garde au Cegep de Saint-Jerome (The Evaluation of Programs of Study in Quebec. Case Study: Child Care Education Programs in CEGEP Saint Jerome).

    ERIC Educational Resources Information Center

    Quebec Commission on the Evaluation of Collegiate Teaching (Quebec).

    Providing a case study of instructional organization and evaluation processes in Quebec's "Colleges d'enseignement general et professionnel" (CEGEPs), this report describes the provincial Commission on the Evaluation of Collegiate Teaching and a 1994 evaluation of "Techniques d'education en services de garde" (TESG), or child…

  5. Density functional theory-based simulations of sum frequency generation spectra involving methyl stretching vibrations: effect of the molecular model on the deduced molecular orientation and comparison with an analytical approach

    NASA Astrophysics Data System (ADS)

    Cecchet, F.; Lis, D.; Caudano, Y.; Mani, A. A.; Peremans, A.; Champagne, B.; Guthmuller, J.

    2012-03-01

    The knowledge of the first hyperpolarizability tensor elements of molecular groups is crucial for a quantitative interpretation of the sum frequency generation (SFG) activity of thin organic films at interfaces. Here, the SFG response of the terminal methyl group of a dodecanethiol (DDT) monolayer has been interpreted on the basis of calculations performed at the density functional theory (DFT) level of approximation. In particular, DFT calculations have been carried out on three classes of models for the aliphatic chains. The first class of models consists of aliphatic chains, containing from 3 to 12 carbon atoms, in which only one methyl group can freely vibrate, while the rest of the chain is frozen by a strong overweight of its C and H atoms. This enables us to localize the probed vibrational modes on the methyl group. In the second class, only one methyl group is frozen, while the entire remaining chain is allowed to vibrate. This enables us to analyse the influence of the aliphatic chain on the methyl stretching vibrations. Finally, the dodecanethiol (DDT) molecule is considered, for which the effects of two dielectrics, i.e. n-hexane and n-dodecane, are investigated. Moreover, DDT calculations are also carried out by using different exchange-correlation (XC) functionals in order to assess the DFT approximations. Using the DFT IR vectors and Raman tensors, the SFG spectrum of DDT has been simulated and the orientation of the methyl group has then been deduced and compared with that obtained using an analytical approach based on a bond additivity model. This analysis shows that when using DFT molecular properties, the predicted orientation of the terminal methyl group tends to converge as a function of the alkyl chain length and that the effects of the chain as well as of the dielectric environment are small. Instead, a more significant difference is observed when comparing the DFT-based results with those obtained from the analytical approach, thus indicating

  6. Density functional theory-based simulations of sum frequency generation spectra involving methyl stretching vibrations: effect of the molecular model on the deduced molecular orientation and comparison with an analytical approach.

    PubMed

    Cecchet, F; Lis, D; Caudano, Y; Mani, A A; Peremans, A; Champagne, B; Guthmuller, J

    2012-03-28

    The knowledge of the first hyperpolarizability tensor elements of molecular groups is crucial for a quantitative interpretation of the sum frequency generation (SFG) activity of thin organic films at interfaces. Here, the SFG response of the terminal methyl group of a dodecanethiol (DDT) monolayer has been interpreted on the basis of calculations performed at the density functional theory (DFT) level of approximation. In particular, DFT calculations have been carried out on three classes of models for the aliphatic chains. The first class of models consists of aliphatic chains, containing from 3 to 12 carbon atoms, in which only one methyl group can freely vibrate, while the rest of the chain is frozen by a strong overweight of its C and H atoms. This enables us to localize the probed vibrational modes on the methyl group. In the second class, only one methyl group is frozen, while the entire remaining chain is allowed to vibrate. This enables us to analyse the influence of the aliphatic chain on the methyl stretching vibrations. Finally, the dodecanethiol (DDT) molecule is considered, for which the effects of two dielectrics, i.e. n-hexane and n-dodecane, are investigated. Moreover, DDT calculations are also carried out by using different exchange-correlation (XC) functionals in order to assess the DFT approximations. Using the DFT IR vectors and Raman tensors, the SFG spectrum of DDT has been simulated and the orientation of the methyl group has then been deduced and compared with that obtained using an analytical approach based on a bond additivity model. This analysis shows that when using DFT molecular properties, the predicted orientation of the terminal methyl group tends to converge as a function of the alkyl chain length and that the effects of the chain as well as of the dielectric environment are small. Instead, a more significant difference is observed when comparing the DFT-based results with those obtained from the analytical approach, thus indicating

  7. Evaluation of Little Ice Age cooling in Western Central Andes, suggested by paleoELAs, in contrast with global warming since late 19th century deduced from instrumental records

    NASA Astrophysics Data System (ADS)

    Ubeda, Jose; Palacios, David; Campos, Néstor; Giraldez, Claudia; García, Eduardo; Quiros, Tatiana

    2015-04-01

    This paper attempts to evaluate climate cooling (°C) during the glacial expansion phases using the product GTV•ΔELA, where GTV is the vertical air temperature gradient (°C/m) and ΔELA (m) the difference in level observed between the Equilibrium Line Altitude (ELA) reconstructions for current and past glaciers. With this aim the Area x Altitude Balance Ratio-(AABR) method was used to produce reconstructions of present ELAs (2002-2010) and paleoELAs corresponding to the last glacier advance phase. The reconstructions were produced in three study areas located along a N-S transect of the western cordillera in the Central Andes: the south-western sector of the Nevado Hualcán (9°S, 77°W; Giráldez 2011); the southern slope of the Cordillera Pariaqaqa (12°S, 76°W; Quirós, 2013) and the NW, NE, SE and SW quadrants of the Nevado Coropuna (16°S, 72°W; García 2013; Úbeda 2011; Campos, 2012). The three mountains exceed 6000 m altitude, their summit areas are covered by glaciers, and on their slopes there are existing well-conserved moraines deposited by the last advances near the present front of the ice masses. Although there are no absolute dates to confirm this hypothesis, it has been assumed that the last glacial advances occurred during the Little Ice Age (LIA), which the oxygen isotopes of the Nevado Huascarán (9°S, 77°W) date to the period 1500-1890. For the Hualcán and Pariaqaqa the mean global value of the Earth's GTV (6.5°C/km) was used, considered valid for the Tropics. On the Coropuna a GTV=8.4°C/km was used, based on high resolution sensors installed in situ since 2007 (Úbeda 2011). This gradient is approaching the upper limit of the dry adiabatic gradient (9.8°C/km), as the Coropuna region is more arid than the other case study areas. The climate cooling estimates deduced from the product GTV•ΔELA were compared with the global warming shown by the 1880-2012 series, ΔT=0.85°C, and 1850/1900-2003/2012, ΔT=0.78°C. The differences are

  8. Deducing chemical structure from crystallographically determined atomic coordinates

    PubMed Central

    Bruno, Ian J.; Shields, Gregory P.; Taylor, Robin

    2011-01-01

    An improved algorithm has been developed for assigning chemical structures to incoming entries to the Cambridge Structural Database, using only the information available in the deposited CIF. Steps in the algorithm include detection of bonds, selection of polymer unit, resolution of disorder, and assignment of bond types and formal charges. The chief difficulty is posed by the large number of metallo-organic crystal structures that must be processed, given our aspiration that assigned chemical structures should accurately reflect properties such as the oxidation states of metals and redox-active ligands, metal coordination numbers and hapticities, and the aromaticity or otherwise of metal ligands. Other complications arise from disorder, especially when it is symmetry imposed or modelled with the SQUEEZE algorithm. Each assigned structure is accompanied by an estimate of reliability and, where necessary, diagnostic information indicating probable points of error. Although the algorithm was written to aid building of the Cambridge Structural Database, it has the potential to develop into a general-purpose tool for adding chemical information to newly determined crystal structures. PMID:21775812

  9. Altitude of Mars aurorae deduced from SPICAM limb detections

    NASA Astrophysics Data System (ADS)

    Soret, L.; Gérard, J.-C.; Libert, L.; Shematovich, V. I.; Bisikalo, D. V.; Stiepen, A.; Bertaux, J.-L.

    2015-10-01

    Martian aurorae have been detected with the SPICAM instrument on board Mars Express both in the nadir and the limb viewing modes. In this study, we focus on three limb detections to determine the altitudes of the auroral emissions and their intensities.We then use a model of electron transport in the Martian thermosphere based on a Monte-Carlo method to simulate and to understand the excitation processes leading to these auroral emissions.

  10. Physical properties of conventional explosives deduced from radio frequency emissions

    SciTech Connect

    Harlin, Jeremiah D; Nemzek, Robert

    2008-01-01

    Los Alamos National Laboratory collected broadband radio frequency (RF) electric field change measurements from multiple detonations of high explosives (HE). Three types of HE were used: small cylinders of flake TNT, solid TNT, and PBX-9501. Low frequency signals (<80 MHz) were shot-to-shot repeatable and occurred within the first 100 {mu} s at measured amplitudes of about 2 V m{sup -1} at 35 m distance. High frequency signals (>290 MHz) occurred later, were an order of magnitude lower in signal strength, and were not repeatable. There is a positive correlation between the maximum electric field change and the shock velocity of the HE. The amount of free charge produced in the explosion estimated from the first RF pulse is between 10 and 150 {mu} C. This implies a weakly ionized plasma with temperatures between 2600 and 2900 K.

  11. Polyphyletism of Celastrales deduced from a chloroplast noncoding DNA region.

    PubMed

    Savolainen, V; Spichiger, R; Manen, J F

    1997-04-01

    In a previous study we examined the phylogeny of four families related to the angiosperm order Celastrales based on chloroplast rbcL 5' flanking sequences. We have added here several additional dicots, sampled from 6 of the 7 families of Celastrales sensu Cronquist and 19 putatively related genera. Based on a cladistic analysis of these DNA sequences, the order Celastrales appears polyphyletic: it is here restricted to Celastraceae (including Hippocrateaceae and Brexia) with Parnassia as sister; Aquifoliaceae plus Helwingia are included in Asteridae. Neither Salvadoraceae nor Geissolomataceae, Icacinaceae, Phellinaceae, Aextoxicaceae, Corynocarpaceae, Dichapetalaceae, Stackhousiaceae, or Goupiaceae are related to Celastrales. The usefulness of this noncoding region is discussed and the influence of the A + T content of neighboring bases on the increase of transversions is also observed as previously shown in chloroplast noncoding regions of monocots.

  12. Symmetry energy at subnuclear densities deduced from nuclear masses

    SciTech Connect

    Oyamatsu, Kazuhiro; Iida, Kei

    2010-05-15

    We examine how nuclear masses are related to the density dependence of the symmetry energy. Using a macroscopic nuclear model we calculate nuclear masses in a way dependent on the equation of state of asymmetric nuclear matter. We find by comparison with empirical two-proton separation energies that a smaller symmetry energy at subnuclear densities, corresponding to a larger density symmetry coefficient L, is favored. This tendency, which is clearly seen for nuclei that are neutron-rich, nondeformed, and light, can be understood from the property of the surface symmetry energy in a compressible liquid-drop picture.

  13. Deducing protein function by forensic integrative cell biology.

    PubMed

    Earnshaw, William C

    2013-12-01

    Our ability to sequence genomes has provided us with near-complete lists of the proteins that compose cells, tissues, and organisms, but this is only the beginning of the process to discover the functions of cellular components. In the future, it's going to be crucial to develop computational analyses that can predict the biological functions of uncharacterised proteins. At the same time, we must not forget those fundamental experimental skills needed to confirm the predictions or send the analysts back to the drawing board to devise new ones.

  14. Magnetospheric electric fields deduced from drifting whistler paths.

    NASA Technical Reports Server (NTRS)

    Carpenter, D. L.; Stone, K.; Siren, J. C.; Crystal, T. L.

    1972-01-01

    Results of a study in which the amplitude of the E-W component E sub w of the convection electric field in the nightside magnetosphere has been inferred from the observed cross-L motions of whistler ducts within the plasmasphere, and several ducts distributed over 1 to 2 earth radii in L space and over plus or minus deg 15 around the longitude of the Eights, Antarctica, whistler station have been tracked simultaneously. The method appears capable of resolving fluctuations in E sub w with period T equal to approximately 15 min and rms amplitude as low as 0.05 mV/m. For variations with T greater than 1 hour the method has a sensitivity of the order of 0.01 mV/m. Three case studies are presented, two of which illustrate convection activity associated with relatively isolated substorms. In these two cases E sub w reversed from westward to eastward for a period following the decay of substorm bay activity. In the third case the substorm bay activity was prolonged, and E sub w remained westward and at enhanced levels until local dawn. Evidence was found that, at least in a limited longitudinal sector, perturbing substorm E sub w fields can penetrate deep within the plasmasphere. In two of the case studies comparisons of E sub w and the interplanetary magnetic-field theta component show evidence of a possible relation based on brief (less than or equal to 1 hour) southward excursions but not on long preceding southward events.

  15. Paleoenvironmental conditions in a Travertine Complex deduced from rock magnetism

    NASA Astrophysics Data System (ADS)

    Reinders, Jan; Hambach, Ulrich

    We present a rock magnetic study on ca. 100 specimens from a 7.5 m travertine section (BMH) and a parallel 1.0 m profile (BMP) to evaluate vertical and lateral variations. Concentration dependent parameters and inter-parametric ratios point to varying redox conditions through time and space suggesting local paleoenvironmental rather than paleoclimatic control of the rock magnetic properties.

  16. Dust-gas interaction deduced from Halley multicolor camera observations

    NASA Technical Reports Server (NTRS)

    Huebner, W. F.; Keller, H. U.; Wilhelm, K.; Whipple, Fred L.; Delamere, W. A.; Reitsema, H. J.; Schmidt, H. U.

    1986-01-01

    The dust and gas productions of comet Halley were measured by the dust counter and the mass spectrometers on the Giotto spacecraft. These instruments give only little information about the spatial asymmetry of the activity. The asymmetry in the dust production is clearly evident from the dust jets seen in the Halley Multicolor Camera images. Since the dust is entrained by the gas, gas production must be similarly asymmetric. The intensity profiles along and across several dust jets are related to their source regions on the nucleus. Properties of the dust jets are investigated. A few compact, but highly active source regions on the nucleus produce most of the visible dust and can account for most of the gas produced by the comet.

  17. Debris and meteoroid proportions deduced from impact crater residue analysis

    NASA Technical Reports Server (NTRS)

    Berthoud, Lucinda; Mandeville, Jean-Claude; Durin, Christian; Borg, Janet

    1995-01-01

    This study is a further investigation of space-exposed samples recovered from the LDEF satellite and the Franco-Russian 'Aragatz' dust collection experiment on the Mir Space Station. Impact craters with diameters ranging from 1 to 900 micron were found on the retrieved samples. Elemental analysis of residues found in the impact craters was carried out using Energy Dispersive X-ray spectrometry (EDX). The analyses show evidence of micrometeoroid and orbital debris origins for the impacts. The proportions of these two components vary according to particle size and experimental position with respect to the leading edge of the spacecraft. On the LDEF leading edge 17 percent of the impacts were apparently caused by micrometeoroids and 11 percent by debris; on the LDEF trailing edge 23 percent of the impacts are apparently caused by micrometeoroids and 4 percent consist of debris particles - mostly larger than 3 micron in diameter - in elliptical orbits around the Earth. For Mir, the analyses indicate that micrometeoroids form 23 percent of impacts and debris 9 percent. However, we note that 60-70 percent of the craters are unidentifiable, so the definitive proportions of natural v. man-made particles are yet to be determined. Experiments carried out using a light gas gun to accelerate glass spheres and fragments demonstrate the influence of particle shape on crater morphology. The experiments also show that it is more difficult to analyze the residues produced by an irregular fragment than those produced by a spherical projectile. If the particle is travelling above a certain velocity, it vaporizes upon impact and no residues are left. Simulation experiments carried out with an electrostatic accelerator indicate that this limit is about 14 km/s for Fe particles impacting Al targets. This chemical analysis cut-off may bias interpretations of the relative populations of meteoroid and orbital debris. Oblique impacts and multiple foil detectors provide a higher likelihood of detection of residues as the velocities involved are lower.

  18. Debris and meteoroid proportions deduced from impact crater residue analysis

    SciTech Connect

    Berthoud, L.; Mandeville, J.C.; Durin, C.; Borg, J. |

    1995-02-01

    This study is a further investigation of space-exposed samples recovered from the LDEF satellite and the Franco-Russian `Aragatz` dust collection experiment on the Mir Space Station. Impact craters with diameters ranging from 1 to 900 micron were found on the retrieved samples. Elemental analysis of residues found in the impact craters was carried out using Energy Dispersive X-ray spectrometry (EDX). The analyses show evidence of micrometeoroid and orbital debris origins for the impacts. The proportions of these two components vary according to particle size and experimental position with respect to the leading edge of the spacecraft. On the LDEF leading edge 17 percent of the impacts were apparently caused by micrometeoroids and 11 percent by debris; on the LDEF trailing edge 23 percent of the impacts are apparently caused by micrometeoroids and 4 percent consist of debris particles - mostly larger than 3 micron in diameter - in elliptical orbits around the Earth. For Mir, the analyses indicate that micrometeoroids form 23 percent of impacts and debris 9 percent. However, the authors note that 60-70 percent of the craters are unidentifiable, so the definitive proportions of natural vs. man-made particles are yet to be determined. Experiments carried out using a light gas gun to accelerate glass spheres and fragments demonstrate the influence of particle shape on crater morphology. The experiments also show that it is more difficult to analyze the residues produced by an irregular fragment than those produced by a spherical projectile. If the particle is travelling above a certain velocity, it vaporizes upon impact and no residues are left. Simulation experiments carried out with an electrostatic accelerator indicate that this limit is about 14 km/s for Fe particles impacting Al targets. This chemical analysis cut-off may bias interpretations of the relative populations of meteoroid and orbital debris.

  19. Preliminary results on interstellar reddening as deduced from filter photometry

    NASA Technical Reports Server (NTRS)

    Laget, M.

    1972-01-01

    Filter photometry has been used to derive the interstellar reddening law from stars through the study of a single spectral type, B0. The deficiency in the far ultraviolet flux of a supergiant relative to a main sequence star is compared with the difference in the flux distribution due to a change of one spectral class. Individual interstellar reddening curves show the general feature reported by Stecher (1969) and by Bless and Savage (1970). There is a large amount of scatter in the far ultraviolet which may be partially due to a real difference in interstellar extinction and partially due to observational inaccuracy.

  20. Hazard estimation deduced from GPS and seismic data of Egypt

    NASA Astrophysics Data System (ADS)

    Sayed Mohamed, Abdel-Monem

    Egypt rapidly growing development is accompanied by increasing levels of standard living particular in its urban areas. However, there is a limited experience in quantifying the sources of risk management in Egypt and in designing efficient strategies to keep away serious impacts of earthquakes. From the historical point of view and recent instrumental records, there are some seismo-active regions in Egypt, where some significant earthquakes had occurred in different places. The special tectonic features in Egypt: Aswan, Greater Cairo, Red Sea and Sinai Peninsula regions are the territories of a high seismic risk, which have to be monitored by up-to date technologies. The investigations of the seismic events and interpretations led to evaluate the seismic hazard for disaster prevention and for the safety of the dense populated regions and the vital national projects as the High Dam. In addition to the monitoring of the recent crustal movements, the most powerful technique of satellite geodesy GPS are used where geodetic networks are covering such seismo-active regions. The results from the data sets are compared and combined in order to determine the main characteristics of the deformation and hazard estimation for specified regions. The final compiled output from the seismological and geodetic analysis threw lights upon the geodynamical regime of these seismo-active regions and put Aswan and Greater Cairo under the lowest class according to horizontal crustal strains classifications. This work will serve a basis for the development of so-called catastrophic models and can be further used for catastrophic risk management. Also, this work is trying to evaluate risk of large catastrophic losses within the important regions including the High Dam, strategic buildings and archeological sites. Studies on possible scenarios of earthquakes and losses are a critical issue for decision making in insurance as a part of mitigation measures.

  1. Vegetation density as deduced from ERTS-1 MSS response

    NASA Technical Reports Server (NTRS)

    Wiegand, C. L.; Gausman, H. W.; Cuellar, J. A.; Gerbermann, A. H.; Richardson, A. J.

    1974-01-01

    Reflectance from vegetation increases with increasing vegetation density in the 0.75- to 1.35 micron wavelength interval. Therefore, ERTS-1 bands 6 (0.7 to 0.8 micron) and 7 (0.8 to 1.1 micron) contain information that should relate to the probable yield of crops and the animal carrying capacity of rangeland. The results of an experiment designed specifically to test the relations among leaf area index (LAI), plant population, plant cover and plant height, and the ERTS-1 MSS responses for 3 corn, 10 sorghum, and 10 cotton fields are given. Plant population was as useful as LAI for characterizing the sorghum and corn fields, and plant height was as good as LAI for characterizing cotton fields. These findings generally support the utility of ERTS-1 data for explaining variability in green biomass, harvestable forage and other indicators of productivity.

  2. Asteroidal Differentiation Processes Deduced from Ultramafic Achondrite Ureilite Meteorites

    NASA Technical Reports Server (NTRS)

    Downes, Hilary; Mittlefehldt, David W.; Hudson, Pierre; Romanek, Christopher S.; Franchi, Ian

    2006-01-01

    Ureilites are the second largest achondrite group. They are ultramafic achondrites that have experienced igneous processing whilst retaining some degree of nebula-derived chemical heterogeneity. They differ from other achondrites in that they contain abundant carbon and their oxygen isotope compositions are very heterogeneous and similar to those of the carbonaceous chondrite anhydrous mineral line. Their carbonaceous nature and some compositional characteristics indicative of nebular origin suggest that they are primitive materials that form a link between nebular processes and early periods of planetesimal accretion. However, despite numerous studies, the exact origin of ureilites remains unclear. Current opinion is that they represent the residual mantle of an asteroid that underwent silicate and Fe-Ni-S partial melting and melt removal. Recent studies of short-lived chronometers indicate that the parent asteroid of the ureilites differentiated very early in the history of the Solar System. Therefore, they contain important information about processes that formed small rocky planetesimals in the early Solar System. In effect, they form a bridge between nebula processes and differentiation in small planetesimals prior to accretion into larger planets and so a correct interpretation of ureilite petrogenesis is essential for understanding this critical step.

  3. Deducing Electron Properties from Hard X-Ray Observations

    NASA Technical Reports Server (NTRS)

    Kontar, E. P.; Brown, J. C.; Emslie, A. G.; Hajdas, W.; Holman, G. D.; Hurford, G. J.; Kasparova, J.; Mallik, P. C. V.; Massone, A. M.; McConnell, M. L.; Piana, M.; Prato, M.; Schmahl, E. J.; Suarez-Garcia, E.

    2011-01-01

    X-radiation from energetic electrons is the prime diagnostic of flare-accelerated electrons. The observed X-ray flux (and polarization state) is fundamentally a convolution of the cross-section for the hard X-ray emission process(es) in question with the electron distribution function, which is in turn a function of energy, direction, spatial location and time. To address the problems of particle propagation and acceleration one needs to infer as much information as possible on this electron distribution function, through a deconvolution of this fundamental relationship. This review presents recent progress toward this goal using spectroscopic, imaging and polarization measurements, primarily from the Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI). Previous conclusions regarding the energy, angular (pitch angle) and spatial distributions of energetic electrons in solar flares are critically reviewed. We discuss the role and the observational evidence of several radiation processes: free-free electron-ion, free-free electron-electron, free-bound electron-ion, photoelectric absorption and Compton backscatter (albedo), using both spectroscopic and imaging techniques. This unprecedented quality of data allows for the first time inference of the angular distributions of the X-ray-emitting electrons and improved model-independent inference of electron energy spectra and emission measures of thermal plasma. Moreover, imaging spectroscopy has revealed hitherto unknown details of solar flare morphology and detailed spectroscopy of coronal, footpoint and extended sources in flaring regions. Additional attempts to measure hard X-ray polarization were not sufficient to put constraints on the degree of anisotropy of electrons, but point to the importance of obtaining good quality polarization data in the future.

  4. Total ozone trends deduced from Nimbus 7 TOMS data

    NASA Technical Reports Server (NTRS)

    Stolarski, Richard S.; Mcpeters, Richard D.; Herman, Jay R.; Bloomfield, Peter

    1991-01-01

    The Total Ozone Mapping Spectrometer (TOMS) on the Nimbus 7 satellite has been measuring the total column amount of ozone over the globe for more than 11 years. Recent improvements in the data analysis have led to a technique for determining and removing drift in the calibration such that the data at the end of the record are precise to + or - 1.3 percent (2-sigma) relative to the data at the beginning of the record. A statistical model, including terms for seasonal variation, linear trend, quasi-biennial oscillation, solar cycle and second-order autoregressive noise has been fit to the TOMS time series of total ozone data. The linear trend obtained when this statistical model is fit to the TOMS data averaged between 65 N and 65 S latitudes is -0.26 + or - 0.14 percent/year or -3 percent over the 11.6 year time period from November 1978 to May 1990. The trend is near zero (0.0002 + or - 0.2 percent/year) at the equator and increases toward both poles.

  5. Total ozone trends deduced from Nimbus 7 TOMS data

    SciTech Connect

    Stolarski, R.S.; McPeters, R.D.; Herman, J.R. ); Bloomfield, P. )

    1991-06-01

    The Total Ozone Mapping Spectrometer (TOMS) on the Nimbus 7 satellite has been measuring the total column amount of ozone over the globe for more than 11 years. Recent improvements in the data analysis have led to a technique for determining and removing drift in the calibration such that the data at the end of the record are precise to {plus minus} 1.3% (2{sigma}) relative to the data at the beginning of the record. A statistical model, including terms for seasonal variation, linear trend, quasi-biennial oscillation, solar cycle and second-order autoregressive noise has been fit to the TOMS time series of total ozone data. The linear trend obtained when this statistical model is fit to the TOMS data averaged between 65N and 65S latitudes is {minus}0.26 {plus minus} 0.14%/year or {minus}3% over the 11.6 year time period from November, 1978 through May, 1990. The trend is near zero (0.0002 {plus minus} 0.2%/year) at the equator and increases towards both poles. At 50N the annually averaged trend is {minus}0.5 {plus minus} .21%/year. The 50N trend over the 11.6 year time period shows a strong seasonal variation from more than {minus}0.8%/year in winter and early spring (February and March) to about {minus}0.2%/year in summer (July and August).

  6. Deducing Electron Properties from Hard X-Ray Observations

    NASA Technical Reports Server (NTRS)

    Kontar, E. P.; Brown, J. C.; Emslie, A. G.; Hajdas, W.; Holman, G. D.; Hurford, G. J.; Kasparova, J.; Mallik, P. C. V.; Massone, A. M.; McConnell, M. L.; hide

    2011-01-01

    X-radiation from energetic electrons is the prime diagnostic of flare-accelerated electrons. The observed X-ray flux (and polarization state) is fundamentally a convolution of the cross-section for the hard X-ray emission process(es) in question with the electron distribution function, which is in turn a function of energy, direction, spatial location and time. To address the problems of particle propagation and acceleration one needs to infer as much information as possible on this electron distribution function, through a deconvolution of this fundamental relationship. This review presents recent progress toward this goal using spectroscopic, imaging and polarization measurements, primarily from the Reuven Ramaty High Energy Solar Spectroscopic Imager (RHESSI). Previous conclusions regarding the energy, angular (pitch angle) and spatial distributions of energetic electrons in solar flares are critically reviewed. We discuss the role and the observational evidence of several radiation processes: free-free electron-ion, free-free electron-electron, free-bound electron-ion, photoelectric absorption and Compton backscatter (albedo), using both spectroscopic and imaging techniques. This unprecedented quality of data allows for the first time inference of the angular distributions of the X-ray-emitting electrons and improved model-independent inference of electron energy spectra and emission measures of thermal plasma. Moreover, imaging spectroscopy has revealed hitherto unknown details of solar flare morphology and detailed spectroscopy of coronal, footpoint and extended sources in flaring regions. Additional attempts to measure hard X-ray polarization were not sufficient to put constraints on the degree of anisotropy of electrons, but point to the importance of obtaining good quality polarization data in the future.

  7. Deducing trapdoor primitives in public key encryption schemes

    NASA Astrophysics Data System (ADS)

    Pandey, Chandra

    2005-03-01

    Semantic security of public key encryption schemes is often interchangeable with the art of building trapdoors. In the frame of reference of Random Oracle methodology, the "Key Privacy" and "Anonymity" has often been discussed. However to a certain degree the security of most public key encryption schemes is required to be analyzed with formal proofs using one-way functions. This paper evaluates the design of El Gamal and RSA based schemes and attempts to parallelize the trapdoor primitives used in the computation of the cipher text, thereby magnifying the decryption error δp in the above schemes.

  8. Climate change deduced from isotopes in tree rings

    SciTech Connect

    Pendall, E.G.; Leavitt, S.W.

    1997-11-01

    This paper describes the theory of carbon, hydrogen, and oxygen isotopic signatures in cellulose for the purpose of paleoclimatic reconstruction. Mechanisms governing tree ring cellulose isotopic variability are investigated, and applications to the southwestern United States are delineated. A monitoring program of pinyon trees and comparison to climatic parameters is briefly described. Variables measured included deviations in hydrogen and oxygen-18 isotopic composition in precipitation, soil water, stem and leaf water, and atmospheric vapor. Water from phloem tissue was found to be isotopically identical to the xylem sap, suggesting that cellulose precursors can exchange isotopically with source water before cellulose is made in the trunk, thus removing most of the isotopic signal from the leaves. Overall results suggest that, on arid sites in the southwestern US receiving adequate summer rain, a precipitation seasonality signal may be recorded. 21 refs., 2 figs.

  9. Magmatic Processes and Systems Deduced from Single Crystals

    NASA Astrophysics Data System (ADS)

    Davidson, J.; Bezard, R. C.; Morgan, D. J.; Ginibre, C.

    2014-12-01

    When crystals grow in liquids the composition of their outermost layer will reflect that of the host with which they are in equilibrium and will therefore record the liquid composition, pressure and temperature.. Following separation from their sources, magmas differentiate. This change in liquid composition is driven largely by crystallisation in response to cooling or decompression. Other open system processes such as mixing and contamination are common. These can lead to abrupt changes in trace element and isotopic composition, accompanied by petrographic features, such as dissolution surfaces or zones of melt inclusions. Where such careful mineral-scale studies have been performed, the prevalence of open system processes is clear. In many cases these are shown by core-rim isotopic variations. Crystal-scale compositional variations in the context of whole rock compositions and petrography have allowed us to show crustal assimilation even from regions of supposedly oceanic crust such as the Lesser Antilles. In tandem with tracking magma evolution, core-rim analyses of appropriate crystals have also provided diffusion profiles which reflect timescales of magmatic processes. A key point, long recognised by Bruce Marsh, is that in situ geochemical data should be considered in a petrographic context in order to gain the most (and most credible) insights on the workings of magma systems from hand specimen to whole volcano/pluton scales: The petrographic microscope is not dead yet Identification of magmatic processes from in situ scrutiny allows us to synthesise the architectures and inner workings of magma systems. The evidence for interaction among magmas in many systems is compelling and suggests that many exist as stacked dike-sill arrangements with wall-rock focussed crystal growth and mush zones. These are consistent with many of the systematics suggested some time ago by Bruce Marsh

  10. Habitat-specific foraging of prothonotary warblers: Deducing habitat quality

    USGS Publications Warehouse

    Lyons, J.E.

    2005-01-01

    Foraging behavior often reflects food availability in predictable ways. For example, in habitats where food availability is high, predators should attack prey more often and move more slowly than in habitats where food availability is low. To assess relative food availability and habitat quality, I studied the foraging behavior of breeding Prothonotary Warblers (Protonotaria citrea) in two forest habitat types, cypress-gum swamp forest and coastal-plain levee forest. I quantified foraging behavior with focal animal sampling and continuous recording during foraging bouts. I measured two aspects of foraging behavior: 1) prey attack rate (attacks per minute), using four attack maneuvers (glean, sally, hover, strike), and 2) foraging speed (movements per minute), using three types of movement (hop, short flight [???1 m], long flight [>1 m]). Warblers attacked prey more often in cypress-gum swamp forest than in coastal-plain levee forest. Foraging speed, however, was not different between habitats. I also measured foraging effort (% time spent foraging) and relative frequency of attack maneuvers employed in each habitat; neither of these variables was influenced by forest type. I conclude that Prothonotary Warblers encounter more prey when foraging in cypress-gum swamps than in coastal-plain levee forest, and that greater food availability results in higher density and greater reproductive success for birds breeding in cypress-gum swamp.

  11. Deducing acidification rates based on short-term time series

    PubMed Central

    Lui, Hon-Kit; Arthur Chen, Chen-Tung

    2015-01-01

    We show that, statistically, the simple linear regression (SLR)-determined rate of temporal change in seawater pH (βpH), the so-called acidification rate, can be expressed as a linear combination of a constant (the estimated rate of temporal change in pH) and SLR-determined rates of temporal changes in other variables (deviation largely due to various sampling distributions), despite complications due to different observation durations and temporal sampling distributions. Observations show that five time series data sets worldwide, with observation times from 9 to 23 years, have yielded βpH values that vary from 1.61 × 10−3 to −2.5 × 10−3 pH unit yr−1. After correcting for the deviation, these data now all yield an acidification rate similar to what is expected under the air-sea CO2 equilibrium (−1.6 × 10−3 ~ −1.8 × 10−3 pH unit yr−1). Although long-term time series stations may have evenly distributed datasets, shorter time series may suffer large errors which are correctable by this method. PMID:26143749

  12. Paleorecharge by the Niger River (Mali) deduced from groundwater geochemistry

    SciTech Connect

    Fontes, J.C. ); Andrews, J.N. ); Edmunds, W.M. ); Guerre, A. ); Travi, Y. )

    1991-02-01

    Stable isotopes, major elements, trace elements, and noble gases in groundwater from northern Mali permit a reconstruction of the paleohydrology of the Sahelian and sub-Saharan regions of west Africa. Major floods of the Niger River during the more humid episodes of the Holocene, probably associated with northward migration of the interior river delta (flood zone), were responsible for groundwater recharge. A marine aerosol input, somewhat modified by rock-water interaction, dominates the groundwater chemistry. stable isotope compositions distinguish the Saharan zone groundwater from waters recharged in the Sahelian zone south of the Azaouad ridge. Carbon 14 dating shows that whereas the latter are modern, the Saharan zone was recharged in the Early to Middle Holocene. Nobel gas contents suggest that recharge north of Azaouad occurred under cooler climatic conditions than presently prevail.

  13. Properties of the lithosphere and asthenosphere deduced from geoid observations

    NASA Technical Reports Server (NTRS)

    Turcotte, D. L.

    1985-01-01

    Data from the GEOS-3 and SEASAT Satellites provided a very accurate geoid map over the oceans. Broad bathymetric features in the oceans such as oceanic swells and plateaus are fully compensated. It is shown that the geoid anomalies due to the density structures of the lithosphere are proportional to the first moment of the density distribution. The deepening of the ocean basins is attributed to thermal isostasy. The thickness of the oceanic lithosphere increases with age due to the loss of heat to the sea floor. Bathymetry and the geoid provide constraints on the extent of this heat loss. Offsets in the geoid across major fracture zones can also be used to constrain this problem. Geoid bathymetry correlations show that the Hawaiian and Bermuda swells and the Cape Verde Rise are probably due to lithospheric thinning.

  14. Dust-gas interaction deduced from Halley multicolour camera observations

    SciTech Connect

    Huebner, W.F.; Delamere, W.A.; Keller, H.U.; Reitsema, H.J.; Schmidt, H.U.; Whipple, F.L.; Wilhelm, K.

    1986-01-01

    The dust and gas productions of Comet Halley were measured by the dust counter and the mass spectrometers on the Giotto spacecraft. These instruments give only little information about the spatial asymmetry of the activity. The asymmetry in the dust production is clearly evident from the dust jets seen in the Halley Multicolour Camera images. Since the dust is entrained by the gas, production must be similarly asymmetric. The intensity profiles along and across several dust jets are related to their source regions on the nucleus. Properties of the dust jets are investigated. A few compact, but highly active source regions on the nucleus produce most of the visible dust and can account for most of the gas produced by the comet. 2 refs.

  15. Deduced Inference in the Analysis of Experimental Data

    ERIC Educational Resources Information Center

    Bird, Kevin D.

    2011-01-01

    Any set of confidence interval inferences on J - 1 linearly independent contrasts on J means, such as the two comparisons [mu][subscript 1] - [mu][subscript 2] and [mu][subscript 2] - [mu][subscript 3] on 3 means, provides a basis for the deduction of interval inferences on all other contrasts, such as the redundant comparison [mu][subscript 1] -…

  16. 350 Year Cloud Reconstruction Deduced from Northeast Caribbean Coral Proxies

    NASA Astrophysics Data System (ADS)

    Winter, A.; Sammarco, P. W.; Mikolajewicz, U.; Jury, M.; Zanchettin, D.

    2014-12-01

    Clouds are a major factor influencing the global climate and its response to external forcing through their implications for the global hydrological cycle, and hence for the planetary radiative budget. Clouds also contribute to regional climates and their variability through, e.g., the changes they induce in regional precipitation patterns. There have been very few studies of decadal and longer-term changes in cloud cover in the tropics and sub-tropics, both over land and the ocean. In the tropics, there is great uncertainty regarding how global warming will affect cloud cover. Observational satellite data are too short to unambiguously discern any temporal trends in cloud cover. Corals generally live in well-mixed coastal regions and can often record environmental conditions of large areas of the upper ocean. This is particularly the case at low latitudes. Scleractinian corals are sessile, epibenthic fauna, and the type of environmental information recorded at the location where the coral has been living is dependent upon the species of coral considered and proxy index of interest. Skeletons of scleractinian corals are considered to provide among the best records of high-resolution (sub-annual) environmental variability in the tropical and sub-tropical oceans. Zooxanthellate hermatypic corals in tropical and sub-tropical seas precipitate CaCO3 skeletons as they grow. This growth is made possible through the manufacture of CaCO3crystals, facilitated by the zooxanthellae. During the process of crystallization, the holobiont binds carbon of different isotopes into the crystals. Stable carbon isotope concentrations vary with a variety of environmental conditions. In the Caribbean, d13C in corals of the species Montastraea faveolata can be used as a proxy for changes in cloud cover. In this contribution, we will demonstrate that the stable isotope 13C varies concomitantly with cloud cover for the northeastern Caribbean region. Using this proxy we have been able to reconstruct cloud cover conditions back to the year 1760 and thus determine historical cloud cover prior to the recent use of instrumental records. We will also discuss how our coral proxy record of cloud cover compares to paleo-climate model runs for the same time period.

  17. Tracer transport by the diabatic circulation deduced from satellite observations

    NASA Technical Reports Server (NTRS)

    Solomon, S.; Kiehl, J. T.; Garcia, R. R.; Grose, W.

    1986-01-01

    Nimbus-7 sensor data were used to track the diabatic circulation in the stratosphere to study the advective transport of CH4 and N2O as tracer species. Advective transport by the mean circulation was found to be a function of the temperature field and associated deviations from radiative equilibrium. A photochemical model was applied to account for the disappearance of the tracer species from the stratosphere. Comparisons between the SAMS data and modeling on the basis of the chemical loss rates of the tracers and the LIMS circulation data showed that the model predictions underestimated the resident abundances, although the global distributions and circulations exhibited a good match.

  18. Rain patterns motion over a region deduced from radar measurements

    NASA Astrophysics Data System (ADS)

    Pawlina, A.

    1986-04-01

    The knowledge of spatial and temporal evolution of precipitation patterns becomes important in the design of communication systems operating at the frequencies above 10 GHz (SHF) that have to counteract the attenuation by rain. One year of ground rain data obtained from weather radar measurements performed near Milan in Italy is analyzed to find out the preferential directions of motion of precipitation patterns over the observed region. The pattern motion is estimated by means of the cross-correlation procedure applied to 461 pairs of ground rain maps 18 minutes apart, originating from 88 separate rain events. The average velocity field of pattern displacement is found, showing an evident polarization towards east directions. The results may be seen as a contribution to the radio-climatological characterization of the region, aimed to improve rain-attenuation models required in the engineering of the SHF systems.

  19. Nucleic-acid Structural Deformability Deduced from Anisotropic Displacement Parameters†

    PubMed Central

    Peckham, Heather E.; Olson, Wilma K.

    2011-01-01

    The growing numbers of very well resolved nucleic-acid crystal structures with anisotropic displacement parameters provide an unprecedented opportunity to learn about the natural motions of DNA and RNA. Here we report a new Monte-Carlo approach that takes direct account of this information to extract the distortions of covalent structure, base pairing, and dinucleotide geometry intrinsic to regularly organized double-helical molecules. We present new methods to test the validity of the anisotropic parameters and examine the apparent deformability of a variety of structures, including several A, B, and Z DNA duplexes, an AB helical intermediate, an RNA, a ligand-DNA complex, and an enzyme-bound DNA. The rigid-body parameters characterizing the positions of the bases in the structures mirror the mean parameters found when atomic motion is taken into account. The base-pair fluctuations intrinsic to a single structure, however, differ from those extracted from collections of nucleic-acid structures, although selected base-pair steps undergo conformational excursions along routes suggested by the ensembles. The computations reveal surprising new molecular insights, such as the stiffening of DNA and concomitant separation of motions of contacted nucleotides on opposite strands by the binding of Escherichia coli endonuclease VIII, that suggest how the protein may direct enzymatic action. PMID:21280021

  20. Total ozone trends deduced from Nimbus 7 TOMS data

    NASA Technical Reports Server (NTRS)

    Stolarski, Richard S.; Mcpeters, Richard D.; Herman, Jay R.; Bloomfield, Peter

    1991-01-01

    The Total Ozone Mapping Spectrometer (TOMS) on the Nimbus 7 satellite has been measuring the total column amount of ozone over the globe for more than 11 years. Recent improvements in the data analysis have led to a technique for determining and removing drift in the calibration such that the data at the end of the record are precise to + or - 1.3 percent (2-sigma) relative to the data at the beginning of the record. A statistical model, including terms for seasonal variation, linear trend, quasi-biennial oscillation, solar cycle and second-order autoregressive noise has been fit to the TOMS time series of total ozone data. The linear trend obtained when this statistical model is fit to the TOMS data averaged between 65 N and 65 S latitudes is -0.26 + or - 0.14 percent/year or -3 percent over the 11.6 year time period from November 1978 to May 1990. The trend is near zero (0.0002 + or - 0.2 percent/year) at the equator and increases toward both poles.

  1. Site of iodination in hyperplastic thyroid glands deduced from autoradiographs

    SciTech Connect

    Wollman, S.H.; Ekholm, R.

    1981-06-01

    We have tried to ascertain the site of iodination in the chronically stimulated, hyperplastic thyroid gland of rats. Rats were fed propylthiouracil in a commercial rat diet for 10 days. Then the diet was changed to a low iodine diet for 5 days. To label the gland, 10 mCi of 125I-iodide was injected into the left heart ventricle. Ten seconds later the animal was perfused through the left ventricle with a fixative solution containing a goitrogen to block further iodination, and stable iodide to help extract uncombined radioiodide. Electron microscopic autoradiographs prepared from the fixed thyroids show strong labeling over the lumen of the follicle and no consistent labeling of any other site or organelle. We conclude that the site of iodination in the chronically stimulated, hyperplastic thyroid is the follicular lumen, i.e. the same as that in the normal gland.

  2. Nucleic-acid structural deformability deduced from anisotropic displacement parameters.

    PubMed

    Peckham, Heather E; Olson, Wilma K

    2011-04-01

    The growing numbers of very well resolved nucleic-acid crystal structures with anisotropic displacement parameters provide an unprecedented opportunity to learn about the natural motions of DNA and RNA. Here we report a new Monte-Carlo approach that takes direct account of this information to extract the distortions of covalent structure, base pairing, and dinucleotide geometry intrinsic to regularly organized double-helical molecules. We present new methods to test the validity of the anisotropic parameters and examine the apparent deformability of a variety of structures, including several A, B, and Z DNA duplexes, an AB helical intermediate, an RNA, a ligand-DNA complex, and an enzyme-bound DNA. The rigid-body parameters characterizing the positions of the bases in the structures mirror the mean parameters found when atomic motion is taken into account. The base-pair fluctuations intrinsic to a single structure, however, differ from those extracted from collections of nucleic-acid structures, although selected base-pair steps undergo conformational excursions along routes suggested by the ensembles. The computations reveal surprising new molecular insights, such as the stiffening of DNA and concomitant separation of motions of contacted nucleotides on opposite strands by the binding of Escherichia coli endonuclease VIII, which suggest how the protein may direct enzymatic action.

  3. A Single 30-s Stretch Is Sufficient to Inhibit Maximal Voluntary Strength

    ERIC Educational Resources Information Center

    Winchester, Jason B.; Nelson, Arnold G.; Kokkonen, Joke

    2009-01-01

    While it has been well established that an acute stretching program can inhibit maximal muscle performance, the amount of stretching needed to produce the deleterious response is unknown. Therefore this study examined the dose-response relationship between acute stretching and strength inhibition. Eighteen college students performed a one…

  4. SuhB Associates with Nus Factors To Facilitate 30S Ribosome Biogenesis in Escherichia coli

    PubMed Central

    Singh, Navjot; Bubunenko, Mikhail; Smith, Carol; Abbott, David M.; Stringer, Anne M.; Shi, Ronald; Court, Donald L.

    2016-01-01

    ABSTRACT A complex of highly conserved proteins consisting of NusB, NusE, NusA, and NusG is required for robust expression of rRNA in Escherichia coli. This complex is proposed to prevent Rho-dependent transcription termination by a process known as “antitermination.” The mechanism of this antitermination in rRNA is poorly understood but requires association of NusB and NusE with a specific RNA sequence in rRNA known as BoxA. Here, we identify a novel member of the rRNA antitermination machinery: the inositol monophosphatase SuhB. We show that SuhB associates with elongating RNA polymerase (RNAP) at rRNA in a NusB-dependent manner. Although we show that SuhB is required for BoxA-mediated antitermination in a reporter system, our data indicate that the major function of the NusB/E/A/G/SuhB complex is not to prevent Rho-dependent termination of rRNA but rather to promote correct rRNA maturation. This occurs through formation of a SuhB-mediated loop between NusB/E/BoxA and RNAP/NusA/G. Thus, we have reassigned the function of these proteins at rRNA and identified another key player in this complex. PMID:26980831

  5. Protein-RNA Dynamics in the Central Junction Control 30S Ribosome Assembly.

    PubMed

    Baker, Kris Ann; Lamichhane, Rajan; Lamichhane, Tek; Rueda, David; Cunningham, Philip R

    2016-09-11

    Interactions between ribosomal proteins (rproteins) and ribosomal RNA (rRNA) facilitate the formation of functional ribosomes. S15 is a central domain primary binding protein that has been shown to trigger a cascade of conformational changes in 16S rRNA, forming the functional structure of the central domain. Previous biochemical and structural studies in vitro have revealed that S15 binds a three-way junction of helices 20, 21, and 22, including nucleotides 652-654 and 752-754. All junction nucleotides except 653 are highly conserved among the Bacteria. To identify functionally important motifs within the junction, we subjected nucleotides 652-654 and 752-754 to saturation mutagenesis and selected and analyzed functional mutants. Only 64 mutants with greater than 10% ribosome function in vivo were isolated. S15 overexpression complemented mutations in the junction loop in each of the partially active mutants, although mutations that produced inactive ribosomes were not complemented by overexpression of S15. Single-molecule Förster or fluorescence resonance energy transfer (smFRET) was used to study the Mg(2+)- and S15-induced conformational dynamics of selected junction mutants. Comparison of the structural dynamics of these mutants with the wild type in the presence and absence of S15 revealed specific sequence and structural motifs in the central junction that are important in ribosome function. Copyright © 2016 Elsevier Ltd. All rights reserved.

  6. Magmatism and tectonics in continental Chiloé, Chile (42° 42°30'S)

    NASA Astrophysics Data System (ADS)

    Pankhurst, R. J.; Hervé, F.; Rojas, L.; Cembrano, J.

    1992-04-01

    The Chiloé-Chonos region seems to preserve the oldest depositional events in the fore-arc accretionary complex of the Southeast Pacific margin. There are isolated occurrences of low-grade metamorphic rocks, including slates with a Devonian trilobite fauna and schists that give Rb-Sr evidence of a ca. 290 Ma metamorphism. Pillow basalts and ultramafic rocks may represent parts of the Pan-Thalassic ocean floor on which the Palaeozoic sediments were laid down. Emergence of a magmatic arc is indicated by Jurassic to Early Cretaceous volcanogenic and marine deposits. During the mid-Cretaceous climax of plutonic activity, these were intruded by monzogranites, which here constitute the eastern portion of the North Patagonian batholith. They give Rb-Sr isochron ages of 120-100 Ma (Barremian-Albian). Initial 87Sr/ 86Sr ratios of 0.7040-0.7045, and ɛNdt values of +0.5 to +1.5, indicate a simple petrogenesis with a mantle source. The western part of the batholith is petrologically more primitive, being composed predominantly of tonalite, diorite and gabbro, and initial 87Sr/ 86Sr ratios are more variable. Late Cenozoic movement of the Liquiñe-Ofqui fault zone (LOFZ) generated deep pull-apart basins to the west of the uplifted batholith/basement complex. These were filled by thick marine sequences of volcanogenic debris, indicating the wide extent of a mainly rhyolitic volcanic field during Miocene times. Pliocene tonalite and granodiorite plutons (dated by a Rb-Sr whole-rock isochron at 4.7 ± 0.5 Ma) and Holocene andesite-basalt stratovolcanoes are located along the LOFZ. The latter feature has thus been a major influence on the tectonic evolution of the area. There is no evidence for major post-Palaeozoic compression or crustal shortening.

  7. Low Temperature Physics at Yale in the late 30's through the early 50's

    NASA Astrophysics Data System (ADS)

    Wheeler, Robert

    2006-03-01

    The low temperature program at Yale was initiated by C. T. Lane (1904-1991) in the fall of 1937 when he was appointed to the teaching staff as an instructor in the department of Physics. Following his doctorate from McGill in 1929 he investigated the magnetic susceptibilities of ``soft'' metals supported by the National Research Council of Canada, the Commissioners of the 1851 Exhibition and a Sterling Fellowship at Yale. Arranged by Louis McKeehan, with 5000 from the new George Sheffield research fund, he started the construction of a Kapitza type helium liquefier. The machine was largely completed in the fall of 1939, yet liquid helium was not made until early December 1940 due to the need for extensive on line purification of the gas. Returning in 1945 from war research, Lane and Henry A. Fairbank (Ph.D 1944) continued the metals work along with new thrusts into Second Sound , properties of helium^ three impurities in liquid helium and starting in the 50's on rotating He II. In 1933 both Lane and Onsager were awarded Sterling Fellowships, which initiated a stimulating experimental- theoretical exchange continuing until they both retired. The best-known example was the rediscovery at Yale of the deHaas-van Alphen effect, previously observed only in bismuth, in zinc; where upon Onsager and his students provided new insights into our understanding of the Fermi surface of metals. With the development of new instrumentation one observed vast changes in experimental style during this period. The evolution of the production of liquid helium from Lane's device though the Collins machine to the commodity business of today now makes experiments of huge size and importance possible.

  8. Effect of time of day on aerobic contribution to the 30-s Wingate test performance.

    PubMed

    Souissi, Nizar; Bessot, Nicolas; Chamari, Karim; Gauthier, Antoine; Sesboüé, Bruno; Davenne, Damien

    2007-01-01

    The purpose of this study was to evaluate the effects of time of day on aerobic contribution during high-intensity exercise. A group of 11 male physical education students performed a Wingate test against a resistance of 0.087 kg . kg(-1) body mass. Two different times of day were chosen, corresponding to the minimum (06:00 h) and the maximum (18:00 h) levels of power. Oxygen uptake (.VO(2)) was recorded breath by breath during the test (30 sec). Blood lactate concentrations were measured at rest, just after the Wingate test, and again 5 min later. Oral temperature was measured before each test and on six separate occasions at 02:00, 06:00, 10:00, 14:00, 18:00, and 22:00 h. A significant circadian rhythm was found in body temperature with a circadian acrophase at 18:16+/-00:25 h as determined by cosinor analysis. Peak power (P(peak)), mean power (P(mean)), total work done, and .VO(2) increased significantly from morning to afternoon during the Wingate Test. As a consequence, aerobic contribution recorded during the test increased from morning to afternoon. However, no difference in blood lactate concentrations was observed from morning to afternoon. Furthermore, power decrease was greater in the morning than afternoon. Altogether, these results indicate that the time-of-day effect on performances during the Wingate test is mainly due to better aerobic participation in energy production during the test in the afternoon than in the morning.

  9. A Single 30-s Stretch Is Sufficient to Inhibit Maximal Voluntary Strength

    ERIC Educational Resources Information Center

    Winchester, Jason B.; Nelson, Arnold G.; Kokkonen, Joke

    2009-01-01

    While it has been well established that an acute stretching program can inhibit maximal muscle performance, the amount of stretching needed to produce the deleterious response is unknown. Therefore this study examined the dose-response relationship between acute stretching and strength inhibition. Eighteen college students performed a one…

  10. Trajectories of marijuana use from adolescence to adulthood predicting unemployment in the mid 30s.

    PubMed

    Lee, Jung Yeon; Brook, Judith S; Finch, Stephen J; Brook, David W

    2015-08-01

    Unemployment (5.5% as of 2015) is a serious social and economic problem in our society. Since marijuana use is an important factor related to unemployment, identifying the trajectory of the use of marijuana may aid intervention programs and research on unemployment. Six hundred seventy-four participants (53% African-Americans, 47% Puerto Ricans) were surveyed (60% females) from ages 14 to 36. The first data collection was held when the participants were students attending schools in the East Harlem area of New York City. We found that the chronic marijuana use (OR = 4.07, p < .001; AOR = 2.58, p < .05) and the late marijuana quitter (OR = 2.91, p < .05) trajectory groups were associated with an increased likelihood of unemployment compared with the no marijuana use trajectory group. The results suggest that those who use marijuana chronically are at greater risk for being unemployed. Consequently, these individuals should have access to and participate in marijuana cessation treatment programs in order to reduce their risk of unemployment. Unemployment intervention programs should also consider focusing on the cessation of the use of marijuana to decrease the likelihood of later unemployment. © American Academy of Addiction Psychiatry.

  11. Materials Data on H30S2O21 (SG:8) by Materials Project

    SciTech Connect

    Kristin Persson

    2016-07-14

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  12. Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.

    PubMed

    Thomas, M P; Topham, C M; Kowlessur, D; Mellor, G W; Thomas, E W; Whitford, D; Brocklehurst, K

    1994-06-15

    Chymopapain M, the monothiol cysteine proteinase component of the chymopapain band eluted after chymopapains A and B in cation-exchange chromatography, was isolated from the dried latex of Carica papaya and characterized by kinetic and chromatographic analysis. This late-eluted chymopapain is probably a component of the cysteine proteinase fraction of papaya latex discovered by Schack [(1967) Compt. Rend. Trav. Lab. Carlsberg 36, 67-83], named papaya peptidase B by Lynn [(1979) Biochim. Biophys. Acta 569, 193-201] and partially characterized by Polgár [(1981) Biochim. Biophys. Acta 658, 262-269] and is the enzyme with unusual specificity characteristics (papaya proteinase IV) that Buttle, Kembhavi, Sharp, Shute, Rich and Barrett [Biochem. J. (1989) 261, 469-476] claimed to be a previously undetected cysteine proteinase eluted from a cation-exchange column near to the early-eluted chymopapains. A study of the time-dependent chromatographic consequences of thiol-dependent proteolysis of the components of papaya latex is reported. Chymopapain M was isolated by (i) affinity chromatography followed by separation from papain using cation-exchange f.p.l.c. on a Mono S HR5/5 column and (ii) cation-exchange chromatography followed by an unusual variant of covalent chromatography by thiol-disulphide interchange. The existence in chymopapain M of a nucleophilic interactive Cys/His catalytic-site system analogous to those in papain (EC 3.4.22.2) and other cysteine proteinases was deduced from the characteristics shape of the pH-second-order rate constant (k) profiles for its reactions with 2,2'-dipyridyl disulphide and ethyl 2-pyridyl disulphide. Analysis of the pH-k data for the reactions of chymopapain M with the 2-pyridyl disulphides and with 4,4'-dipyridyl disulphide permits the assignment of molecular pKa values of 3.4 and 8.7 to the formation and subsequent dehydronation of the Cys-S-/His-Im+H state of the catalytic site and reveals three other kinetically influential

  13. Lower plate deformation at the Chile Triple Junction from the paleomagnetic record (45°30'S-46°S)

    NASA Astrophysics Data System (ADS)

    Lagabrielle, Yves; Bourgois, Jacques; Dyment, Jerôme; Pelletier, Bernard

    2015-08-01

    During the Chile Triple Junction (CTJ) cruise, geophysical surveys were conducted between 45°S and 48°S, in the region of the Chile Triple Junction (CTJ), where the Nazca and Antarctica Plates are subducting beneath the South American Plate. Near the CTJ, the South Chile Rise (SCR), which separates the Nazca and Antarctica lower plates, consists of three spreading segments trending ~N160°, separated by a series of parallel fracture zones. The active spreading centers of the three segments consist of grabens with various widths and depths, bounded by steep fault scarps. We provide robust data showing that the SCR recorded remote and long-term effects of ridge subduction far from the subduction front. Magnetic profiles, multibeam bathymetric, and seismic data were acquired at intervals of 13 km along a N80°E direction across the SCR during the CTJ cruise of R/V L'Atalante. Deformation of the oceanic lithosphere includes (1) a segmentation of the spreading axes along strike, (2) some ridge jumps, and (3) local constriction and changes in trend of the fracture zone valleys. Off-axis volcanism is observed in places that may suggest a link with an abnormal stress field induced by ridge subduction. The tectonic and volcanic anomalies, which occurred in response to the subduction of the SCR1 axis, may be correlated with geochemical anomalies and slab fragmentation recognized by previous works.

  14. Extracellular glutamate increases in the lateral hypothalamus during meal initiation, and GABA peaks during satiation: microdialysis measurements every 30 s.

    PubMed

    Rada, Pedro; Mendialdua, Ainhoa; Hernandez, Luis; Hoebel, Bartley G

    2003-04-01

    Glutamate injected into the lateral hypothalamus can initiate eating, and gamma-aminobutyric acid (GABA) can stop it. This leads to the hypothesis that glutamate inputs are active at the beginning of a meal, and GABA is released at the end. To test this theory, the authors used microdialysis to sample glutamate and GABA simultaneously before, during, and after a meal. Food-deprived rats ate a meal of chow. Glutamate increased during the first third of the meal, then decreased to below baseline while the rats were still eating. GABA also increased at the start of the meal but continued rising and peaked during the last third of the meal. Glutamate may drive a hypothalamic system for eating, and GABA may oppose it.

  15. Studies of the structure of the Tz = -1 Nuclei 20Na and 30S for explosive astrophysics

    NASA Astrophysics Data System (ADS)

    Wallace, Jennifer Patrita

    Two and three-dimensional seismic data are used to investigate some stratigraphic relationships between sedimentation, deformation and fluid flow at the lateral margin of a gravity detachment system. Three themes were studied. In the Niger Delta, a right-lateral strike-slip fault with a displacement of around 7 km occurs across 75 km. It is interpreted as the lateral strike-slip domain, linked to the updip extensional domain and the downdip compression zone of a gravity detachment system. Structural and kinematic evidence, such as present-day propagating strike-slip faults, for possible future lateral expansions of the lateral strike-slip domain, is described. We expect to observe similar domains at the margins of other preserved gravitational collapse sliding over a detachment whose efficiency in causing downdip slip may vary laterally. Using 3D data from both Mauritania and Nigeria, the interactions between tear faults and deepwater channels are studied. Structural observations combined with channel analysis are performed in four case studies, illustrating that tear faults influence channel development throughout their evolution. Some examples across tear fault zones are presented, and it is expected to find such interactions elsewhere in the world. A simple model of development of vertical fluid flow pipes is established using two examples that are 400 - 600 m wide and ~ 2000 - 2500 m high. Both examples are located at the crest of rollover anticlines and rooted in buried channel-complexes tilted during fold growth. At the crest of the structures and within the connected permeable reservoir intervals of the channel complexes, lateral pressure transfer caused the pore pressure to reach critical levels, leading to hydraulic fracturing of the overburden. Although hydrocarbons may migrate upwards through the consequent chimney systems, the pipes are not necessarily indicators of hydrocarbon charge of the channel complexes.

  16. Early risk factors, job strain, and atherosclerosis among men in their 30s: the Cardiovascular Risk in Young Finns Study.

    PubMed

    Kivimäki, Mika; Hintsanen, Mirka; Keltikangas-Järvinen, Liisa; Elovainio, Marko; Pulkki-Råback, Laura; Vahtera, Jussi; Viikari, Jorma S A; Raitakari, Olli T

    2007-03-01

    We examined whether preemployment influences confounded the association between job strain and atherosclerosis. We assessed biological, familial, and socioeconomic risk factors of coronary heart disease at 12 to 18 years of age and job strain and carotid artery intima-media thickness at 33 to 39 years of age for a cohort of 358 men. Adolescent risk factors predicted adult intima-media thickness but had little effect on the dose-response relation between greater job strain and greater intima-media thickness. Pre-employment influences did not confound the association between job strain and atherosclerosis.

  17. Tectonic and unroofing history of Neogene Manantiales foreland basin deposits, Cordillera Frontal (32°30'S), San Juan Province, Argentina

    NASA Astrophysics Data System (ADS)

    Pérez, Daniel J.

    2001-12-01

    The Miocene Manantiales foreland basin is located in Cordillera Frontal of San Juan, between 32°30' and 33°S. The unroofing study of the synorogenic Miocene deposits provides information about the structural evolution of Cordón de La Ramada fold-and-thrust belt. These Tertiary deposits are represented by the Chinches Formation and comprise seven members (Tc0-Tc6). They are the result of the uplift of Mesozoic sequences that crop out in La Ramada fold-and-thrust belt of the Cordillera Principal. Quaternary deposits unconformably overlying the Chinches Formation are composed of granitic and rhyolitic blocks, and represent the final uplift of the Cordón del Espinacito and a series of out-of-sequence thrusts. The unroofing studies also provide sufficient information to establish the out-of-sequence timing of the deformation at this latitude. Initial deposition of the Tertiary deposits can be dated at about 20 Ma, or early Miocene. Andesitic lavas dated in 9.2±0.3, 10.7±0.7, and 12.7±0.7 Ma unconformably overlie the structure of La Ramada fold-and-thrust belt. These facts constrain the uplift of the High Andes between 20 and 10 Ma at this latitude. The unconformity between Tertiary and Quaternary deposits suggests final uplift during Pliocene-Pleistocene times.

  18. Pu236(n,f) , Pu237(n,f) , and Pu238(n,f) cross sections deduced from (p,t) , (p,d) , and (p,p') surrogate reactions

    SciTech Connect

    Hughes, R. O.; Beausang, C. W.; Ross, T. J.; Burke, J. T.; Casperson, R. J.; Cooper, N.; Escher, J. E.; Gell, K.; Good, E.; Humby, P.; McCleskey, M.; Saastimoinen, A.; Tarlow, T. D.; Thompson, I. J.

    2014-07-01

    The Pu236(n,f), Pu237(n,f) and Pu238(n,f) cross sections have been inferred by utilizing the surrogate ratio method. Targets of Pu239 and U235 were bombarded with 28.5-MeV protons, and the light ion recoils, as well as fission fragments, were detected using the STARS detector array at the K150 Cyclotron at the Texas A&M cyclotron facility. The (p, tf) reaction on Pu239 and U235 targets was used to deduce the σ (Pu236(n,f))/σ(U232(n,f)) ratio, and the Pu236(n,f) cross section was subsequently determined for En=0.5–7.5 MeV. Similarly, the (p,df) reaction on the same two targets was used to deduce the σ(Pu237(n,f))/σ(U233(n,f)) ratio, and the Pu237(n,f) cross section was extracted in the energy range En=0.5–7 MeV. The Pu238(n,f) cross section was also deduced by utilizing the (p,p') reaction channel on the same targets. There is good agreement with the recent ENDF/B-VII.1 evaluated cross section data for Pu238(n,f) in the range En=0.5–10.5 MeV and for Pu237(n,f) in the range En=0.5–7 MeV; however, the Pu236(n,f) cross section deduced in the present work is higher than the evaluation between 2 and 7 MeV.

  19. Eastern Asian Emissions of Anthropogenic Halocarbons Deduced from Aircraft Concentration Data

    NASA Technical Reports Server (NTRS)

    Palmer, Paul I.; Jacob, Daniel J.; Mickle, Loretta, J.; Blake, Donald R.; Sachse, Glen W.; Fuelberg, Henry E.; Kiley, Christopher M.

    2003-01-01

    The Montreal Protocol restricts production of ozone-depleting halocarbons worldwide. Enforcement of the protocol has relied mainly on annual government statistics of production and consumption of these compounds (bottom-up approach). We show here that aircraft observations of ha1ocarbon:CO enhancement ratios on regional to continental scales can be used to infer halocarbon emissions, providing independent verification of the bottom-up approach. We apply this topdown approach to aircraft observations of Asian outflow &om the TRACE-P mission over the western Pacific (March-April 2001) and derive emissions from eastern Asia (China, Japan, and Korea). We derive an eastern Asian carbon tetrachloride (CCl4) source of 21.5 Gg yr(sup -1), several-fold larger than previous estimates and amounting to -30% of the global budget for this gas. Our emission estimate for CFC-11 from eastern Asia is 50% higher than inventories derived from manufacturing records. Our emission estimates for methyl chloroform (CH3CC13) and CFC-12 are in agreement with existing inventories. For halon 1211 we find only a strong local source originating from the Shanghai area. Our emission estimates for the above gases result in a approximately equal to 40% increase in the ozone depletion potential (ODP) of Asian emissions relative to previous estimates, corresponding to a approximately equal to 10% global increase in ODP.

  20. Traveling wave packets of total electron content disturbances as deduced from global GPS network data

    NASA Astrophysics Data System (ADS)

    Afraimovich, E. L.; Perevalova, N. P.; Voyeikov, S. V.

    2003-07-01

    We identified a specific class of mid-latitude medium-scale traveling ionospheric disturbances (MSTIDs), namely traveling wave packets (TWPs) of total electron content (TEC) disturbances. For the first time, we present the TWP morphology for 105 days 1998-2001. A total number of the TEC series, with a duration of each series of about 2.3h (2h18m), exceeded 700,000. The data were obtained using the technology GLOBDET of global detection of ionospheric disturbances using a global network of GPS receivers, and the technique of GPS interferometry of TIDs, developed at the ISTP SD RAS. It was found that TWPs are observed no more than in 0.1-0.4% of all TEC series, most commonly during the daytime in winter and autumn. TWPs are quasi-periodic oscillations of TEC with a period of around 10-20min, and a time duration of the order of 1h. The TWP amplitudes exceed the amplitudes of ``background'' TEC fluctuations by one order of magnitude, as a minimum. The radius of spatial correlation of TWPs does not exceed 500-600km (3-5 wavelengths). We carried out a detailed analysis of the spatial-temporal properties of TWPs by considering an example of the most conspicuous manifestation of TWPs on October 18, 2001 over California, USA. The velocity and direction of TWP displacement correspond to those of mid-latitude MSTIDs.

  1. Formation des enseignants dans une perspective d'education permanente au benin

    NASA Astrophysics Data System (ADS)

    Agboton, Sébastien; Moussa, Yaya Mede

    1994-05-01

    The history of teacher training in Benin, which provides evidence of social and cultural change, suggests that a system of lifelong education should be introduced which unites formal, non-formal and informal education, all of which already exist in embryo. The education originally given to every member of society was a continuing progression from initiate to initiator, a lifelong education that involved, among other things, knowledge of agricultural production. This traditional system was disrupted by the reorganization of teacher training after 1945. Recurrent training of teachers remained inadequate because of limited external funding for in-service training, the lack of teachers, the suspension of recruitment and the closure of teacher training colleges. The 1975 reform, through the initiative known as Operation Education and Development, envisaged links between school and social environment, allying intellectual work with production. This meant a radical change in the role of the teacher, who was confronted with technical advance and was obliged to enter a difficult continuing process of self-training. Furthermore, population growth and the restrictions imposed by the World Bank have brought about developments in both lifelong learning and apprenticeship. These two elements are brought together under an initiative known as Production Scolaire Artisanale, designed for out-of-school learners and artisans. The author concludes that the creation of a coherent system of voluntary education would be possible in a society that was itself favourably disposed to education.

  2. Stoichiometry of lipid interactions with transmembrane proteins--Deduced from the 3D structures.

    PubMed

    Páli, Tibor; Bashtovyy, Denys; Marsh, Derek

    2006-05-01

    The stoichiometry of the first shell of lipids interacting with a transmembrane protein is defined operationally by the population of spin-labeled lipid chains whose motion is restricted directly by the protein. Interaction stoichiometries have been determined experimentally for a wide range of alpha-helical integral membrane proteins by using spin-label ESR spectroscopy. Here, we determine the spatially defined number of first-shell lipids at the hydrophobic perimeter of integral membrane proteins whose 3D structure has been determined by X-ray crystallography and lipid-protein interactions characterized by spin-labeling. Molecular modeling is used to build a single shell of lipids surrounding transmembrane structures derived from the PDB. Constrained energy optimization of the protein-lipid assemblies is performed by molecular mechanics. For relatively small proteins (up to 7-12 transmembrane helices), the geometrical first shell corresponds to that defined experimentally by perturbation of the lipid-chain dynamics. For larger, multi-subunit alpha-helical proteins, the lipids perturbed directly by the protein may either exceed or be less in number than those that can be accommodated at the intramembranous perimeter. In these latter cases, the motionally restricted spin-labeled lipids can be augmented by intercalation, or can correspond to a specific subpopulation at the protein interface, respectively. For monomeric beta-barrel proteins, the geometrical lipid stoichiometry corresponds to that determined from lipid mobility for a 22-stranded barrel, but fewer lipids are motionally restricted than can be accommodated around an eight-stranded barrel. Deviations from the geometrical first shell, in the beta-barrel case, are for the smaller protein with a highly curved barrel.

  3. A partially differentiated interior for (1) Ceres deduced from its gravity field and shape.

    PubMed

    Park, R S; Konopliv, A S; Bills, B G; Rambaux, N; Castillo-Rogez, J C; Raymond, C A; Vaughan, A T; Ermakov, A I; Zuber, M T; Fu, R R; Toplis, M J; Russell, C T; Nathues, A; Preusker, F

    2016-09-22

    Remote observations of the asteroid (1) Ceres from ground- and space-based telescopes have provided its approximate density and shape, leading to a range of models for the interior of Ceres, from homogeneous to fully differentiated. A previously missing parameter that can place a strong constraint on the interior of Ceres is its moment of inertia, which requires the measurement of its gravitational variation together with either precession rate or a validated assumption of hydrostatic equilibrium. However, Earth-based remote observations cannot measure gravity variations and the magnitude of the precession rate is too small to be detected. Here we report gravity and shape measurements of Ceres obtained from the Dawn spacecraft, showing that it is in hydrostatic equilibrium with its inferred normalized mean moment of inertia of 0.37. These data show that Ceres is a partially differentiated body, with a rocky core overlaid by a volatile-rich shell, as predicted in some studies. Furthermore, we show that the gravity signal is strongly suppressed compared to that predicted by the topographic variation. This indicates that Ceres is isostatically compensated, such that topographic highs are supported by displacement of a denser interior. In contrast to the asteroid (4) Vesta, this strong compensation points to the presence of a lower-viscosity layer at depth, probably reflecting a thermal rather than compositional gradient. To further investigate the interior structure, we assume a two-layer model for the interior of Ceres with a core density of 2,460-2,900 kilograms per cubic metre (that is, composed of CI and CM chondrites), which yields an outer-shell thickness of 70-190 kilometres. The density of this outer shell is 1,680-1,950 kilograms per cubic metre, indicating a mixture of volatiles and denser materials such as silicates and salts. Although the gravity and shape data confirm that the interior of Ceres evolved thermally, its partially differentiated interior indicates an evolution more complex than has been envisioned for mid-sized (less than 1,000 kilometres across) ice-rich rocky bodies.

  4. Features of CO2 fracturing deduced from acoustic emission and microscopy in laboratory experiments

    NASA Astrophysics Data System (ADS)

    Ishida, Tsuyoshi; Chen, Youqing; Bennour, Ziad; Yamashita, Hiroto; Inui, Shuhei; Nagaya, Yuya; Naoi, Makoto; Chen, Qu; Nakayama, Yoshiki; Nagano, Yu

    2016-11-01

    We conducted hydraulic fracturing (HF) experiments on 170 mm cubic granite specimens with a 20 mm diameter central hole to investigate how fluid viscosity affects HF process and crack properties. In experiments using supercritical carbon dioxide (SC-CO2), liquid carbon dioxide (L-CO2), water, and viscous oil with viscosity of 0.051-336.6 mPa · s, we compared the results for breakdown pressure, the distribution and fracturing mechanism of acoustic emission, and the microstructure of induced cracks revealed by using an acrylic resin containing a fluorescent compound. Fracturing with low-viscosity fluid induced three-dimensionally sinuous cracks with many secondary branches, which seem to be desirable pathways for enhanced geothermal system, shale gas recovery, and other processes.

  5. Proton Spectroscopic Factors Deduced from Helium-3 Global Phenomenological and Microscopic Optical Model Potentials

    NASA Astrophysics Data System (ADS)

    Jenny, Lee; Pang, Dan-Yang; Han, Yin-Lu; B. Tsang, M.

    2014-09-01

    Global phenomenological GDP08 and microscopic helium-3 optical model potentials have been recently derived. We evaluate these two potential sets by comparing the elastic scattering data of 25 MeV 3He on 16O, 18O, 19F, 23Na, 24Mg, 25Mg, 26Mg, 27Al, 28Si, 30Si, 31P, 32S, 34S, 35Cl, 37Cl, and 39K isotopes. Using the deuteron angular distributions calculated with the distorted wave Born approximation model, we extract the ground-state proton spectroscopic factors from (3He, d) reactions on the same set of nuclei. The extracted proton spectroscopic factors are compared with the large-basis shell-model calculations.

  6. The optical luminosity function of gamma-ray bursts deduced from ROTSE-III observations

    SciTech Connect

    Cui, X. H.; Wu, X. F.; Wei, J. J.; Yuan, F.; Zheng, W. K.; Liang, E. W.; Akerlof, C. W.; McKay, T. A.; Ashley, M. C. B.; Flewelling, H. A.; Göǧüş, E.; Güver, T.; Kızıloǧlu, Ü.; Pandey, S. B.; Rykoff, E. S.; Rujopakarn, W.; Schaefer, B. E.; Wheeler, J. C.; Yost, S. A. E-mail: xfwu@pmo.ac.cn E-mail: fang.yuan@anu.edu.au E-mail: lew@gxu.edu.cn

    2014-11-10

    We present the optical luminosity function (LF) of gamma-ray bursts (GRBs) estimated from a uniform sample of 58 GRBs from observations with the Robotic Optical Transient Search Experiment III (ROTSE-III). Our GRB sample is divided into two sub-samples: detected afterglows (18 GRBs) and those with upper limits (40 GRBs). We derive R-band fluxes for these two sub-samples 100 s after the onset of the burst. The optical LFs at 100 s are fitted by assuming that the co-moving GRB rate traces the star formation rate. While fitting the optical LFs using Monte Carlo simulations, we take into account the detection function of ROTSE-III. We find that the cumulative distribution of optical emission at 100 s is well described by an exponential rise and power-law decay, a broken power law,and Schechter LFs. A single power-law (SPL) LF, on the other hand, is ruled out with high confidence.

  7. Deducing Wild 2 Components with a Statistical Dataset of Olivine in Chondrite Matrix

    NASA Technical Reports Server (NTRS)

    Frank, D. R.; Zolensky, M. E.; Le, L.

    2012-01-01

    Introduction: A preliminary exam of the Wild 2 olivine yielded a major element distribution that is strikingly similar to those for aqueously altered carbonaceous chondrites (CI, CM, and CR) [1], in which FeO-rich olivine is preferentially altered. With evidence lacking for large-scale alteration in Wild 2, the mechanism for this apparent selectivity is poorly understood. We use a statistical approach to explain this distribution in terms of relative contributions from different chondrite forming regions. Samples and Analyses: We have made a particular effort to obtain the best possible analyses of both major and minor elements in Wild 2 olivine and the 5-30 micrometer population in chondrite matrix. Previous studies of chondrite matrix either include larger isolated grains (not found in the Wild 2 collection) or lack minor element abundances. To overcome this gap in the existing data, we have now compiled greater than 10(exp 3) EPMA analyses of matrix olivine in CI, CM, CR, CH, Kakangari, C2-ungrouped, and the least equilibrated CO, CV, LL, and EH chondrites. Also, we are acquiring TEM/EDXS analyses of the Wild 2 olivine with 500s count times, to reduce relative errors of minor elements with respect to those otherwise available. Results: Using our Wild 2 analyses and those from [2], the revised major element distribution is more similar to anhydrous IDPs than previous results, which were based on more limited statistics (see figure below). However, a large frequency peak at Fa(sub 0-1) still persists. All but one of these grains has no detectable Cr, which is dissimilar to the Fa(sub 0-1) found in the CI and CM matrices. In fact, Fa(sub 0-1) with strongly depleted Cr content is a composition that appears to be unique to Kakangari and enstatite (highly reduced) chondrites. We also note the paucity of Fa(sub greater than 58), which would typically indicate crystallization in a more oxidizing environment [3]. We conclude that, relative to the bulk of anhydrous IDPs, Wild 2 may have received a larger contribution from the Kakangari and/or enstatite chondrite forming regions. Alternatively, Wild 2 may have undergone accretion in an anomalously reducing region, marked by nebular condensation of this atypical forsterite. In [4], a similar conclusion was reached with an Fe-XANES study. We will also use similar lines of reasoning, and our previous conclusions in [5], to constrain the relative contributions of silicates that appear to have been radially transported from different ordinary and carbonaceous chondrite forming regions to the Kuiper Belt. In addition, the widespread depletion of Cr in these FeO-rich (Fa(sub greater than 20)) fragments is consistent with mild thermal metamorphism in Wild 2.

  8. Stratospheric warmings in the Southern Hemisphere deduced from satellite radiation data, 1969-73

    NASA Technical Reports Server (NTRS)

    Quiroz, R. S.

    1974-01-01

    The investigation is largely based on radiation data from the vertical temperature profile radiometers on the satellite NOAA 2. The physical-statistical interpretation of the radiance data is considered. Aspects regarding the nonuniqueness of the minor warming in August 1973 are discussed. It is pointed out that conditions leading to major warmings in the Northern Hemisphere were not satisfied in the Southern Hemisphere during the period from 1969 to 1973.

  9. Accretion timescale and impact history of Mars deduced from the isotopic systematics of martian meteorites

    DOE PAGES

    Borg, Lars E.; Brennecka, Gregory A.; Symes, Steven J. K.

    2015-12-12

    High precision Sm-Nd isotopic analyses have been completed on a suite of 11 martian basaltic meteorites in order to better constrain the age of silicate differentiation on Mars associated with the formation of their mantle sources. Our data is used to evaluate the merits and disadvantages of various mathematical approaches that have been employed in previous work on this topic. Ages determined from the Sm-Nd isotopic systematics of individual samples are strongly dependent on the assumed Nd isotopic composition of the bulk planet. This assumption is problematic given differences observed between the Nd isotopic composition of Earth and chondritic meteoritesmore » and the fact that these materials are both commonly used to represent bulk planetary Nd isotopic compositions. Ages determined from the slope of Sm-146-Nd-142 whole rock isochrons are not dependent on the assumed Nd-142/Nd-144 ratio of the planet, but require the sample suite to be derived from complementary, contemporaneously-formed reservoirs. In this work, we present a mathematical expression that defines the age of formation of the source regions of such a suite of samples that is based solely on the slope of a Nd-143-Nd-142 whole rock isochron and is also independent of any a priori assumptions regarding the bulk isotopic composition of the planet. This expression is also applicable to mineral isochrons and has been used to successfully calculate Nd-143-Nd-142 model crystallization ages of early refractory solids as well as lunar samples. This permits ages to be obtained using only Nd isotopic measurements without the need for Sm-147/Nd-144 isotope dilution determinations. When used in conjunction with high-precision Nd isotopic measurements completed on martian meteorites this expression yields an age of formation of the martian basaltic meteorite source regions of 4504 +/- 6 Ma. Because the Sm-Nd model ages for the formation of martian source regions are commonly interpreted to record the age at which large scale mantle reservoirs formed during planetary differentiation associated with magma ocean solidification, the age determined here implies that magma ocean solidification occurred several tens of millions of years after the beginning of the Solar System. Recent thermal models, however, suggest that Mars-sized bodies cool rapidly in less than similar to 5 Ma after accretion ceases, even in the presence of a thick atmosphere. In assuming these models are correct, an extended period of accretion is necessary to provide a mechanism to keep portions of the martian mantle partially molten until 4504 Ma. Late accretional heating of Mars could either be associated with protracted accretion occurring at a quasi-steady state or alternatively be associated with a late giant impact. If this scenario is correct, then accretion of Mars-sized bodies takes up to 60 Ma and is likely to be contemporaneous with the core formation and possibly the onset of silicate differentiation. This further challenges the concept that isotopic equilibrium is attained during primordial evolution of planets, and may help to account for geochemical evidence implying addition of material into planetary interiors after core formation was completed.« less

  10. Accretion timescale and impact history of Mars deduced from the isotopic systematics of martian meteorites

    SciTech Connect

    Borg, Lars E.; Brennecka, Gregory A.; Symes, Steven J. K.

    2015-12-12

    High precision Sm-Nd isotopic analyses have been completed on a suite of 11 martian basaltic meteorites in order to better constrain the age of silicate differentiation on Mars associated with the formation of their mantle sources. Our data is used to evaluate the merits and disadvantages of various mathematical approaches that have been employed in previous work on this topic. Ages determined from the Sm-Nd isotopic systematics of individual samples are strongly dependent on the assumed Nd isotopic composition of the bulk planet. This assumption is problematic given differences observed between the Nd isotopic composition of Earth and chondritic meteorites and the fact that these materials are both commonly used to represent bulk planetary Nd isotopic compositions. Ages determined from the slope of Sm-146-Nd-142 whole rock isochrons are not dependent on the assumed Nd-142/Nd-144 ratio of the planet, but require the sample suite to be derived from complementary, contemporaneously-formed reservoirs. In this work, we present a mathematical expression that defines the age of formation of the source regions of such a suite of samples that is based solely on the slope of a Nd-143-Nd-142 whole rock isochron and is also independent of any a priori assumptions regarding the bulk isotopic composition of the planet. This expression is also applicable to mineral isochrons and has been used to successfully calculate Nd-143-Nd-142 model crystallization ages of early refractory solids as well as lunar samples. This permits ages to be obtained using only Nd isotopic measurements without the need for Sm-147/Nd-144 isotope dilution determinations. When used in conjunction with high-precision Nd isotopic measurements completed on martian meteorites this expression yields an age of formation of the martian basaltic meteorite source regions of 4504 +/- 6 Ma. Because the Sm-Nd model ages for the formation of martian source regions are commonly interpreted to record the age at which large scale mantle reservoirs formed during planetary differentiation associated with magma ocean solidification, the age determined here implies that magma ocean solidification occurred several tens of millions of years after the beginning of the Solar System. Recent thermal models, however, suggest that Mars-sized bodies cool rapidly in less than similar to 5 Ma after accretion ceases, even in the presence of a thick atmosphere. In assuming these models are correct, an extended period of accretion is necessary to provide a mechanism to keep portions of the martian mantle partially molten until 4504 Ma. Late accretional heating of Mars could either be associated with protracted accretion occurring at a quasi-steady state or alternatively be associated with a late giant impact. If this scenario is correct, then accretion of Mars-sized bodies takes up to 60 Ma and is likely to be contemporaneous with the core formation and possibly the onset of silicate differentiation. This further challenges the concept that isotopic equilibrium is attained during primordial evolution of planets, and may help to account for geochemical evidence implying addition of material into planetary interiors after core formation was completed.

  11. Fishing for biodiversity: novel methanopterin-linked C transfer genes deduced from the Sargasso Sea metagenome.

    PubMed

    Kalyuzhnaya, Marina G; Nercessian, Olivier; Lapidus, Alla; Chistoserdova, Ludmila

    2005-12-01

    The recently generated database of microbial genes from an oligotrophic environment populated by a calculated 1800 major phylotypes (the Sargasso Sea metagenome-SSM) presents a great source for expanding local databases of genes indicative of a specific function. In this article we analyse the SSM for the presence of methanopterin-linked C1 transfer genes that are signature for methylotrophy. We conclude that more than 10 phylotypes possessing genes of interest are present in this environment. The sequences representative of these major phylotypes do not appear to belong to any known microbial group capable of methanopterin-linked C1 transfer. Instead, these sequences separate from all known sequences on phylogenetic trees, pointing toward their affiliation with novel microbial phyla. These data imply a broader distribution of methanopterin-linked functions in the microbial world than has been previously known.

  12. Incorporation of hydrogen in cubic and uniaxial oxidic crystals deduced from polarized Raman scattering

    NASA Astrophysics Data System (ADS)

    Klauer, S.; Wöhlecke, M.

    1994-01-01

    Polarized Raman scattering investigations of the ir OH stretch mode vibration have been performed in a variety of prototype oxidic crystals, KTaO3, SrTiO3, K1-xLixTaO3, TiO2, LiTaO3, with the aim of providing information on the sites of the incorporation of hydrogen. The traditionally used polarized ir absorption is limited by ensemble averages and its lacking sensitivity to crystallographic orientations of defects. Contrarily Raman scattering is sensitive to the symmetry of the dynamical mode. The problem of extracting information on a single defect from an ensemble is treated in the behavior-type theory, which has been developed recently for defects in cubic crystals and was extended to tetragonal and trigonal crystals in the course of these investigations. The principles of the method and the extensive tables are summarized. We describe an experimental method for determining the precise optical alignment of the scattering geometry. In the cubic phase of the perovskites SrTiO3 and KTaO3 with isotropic ir absorption, the Raman data allow us to reject one out of three models, where the protons vibrate between the two next-nearest oxygen ions along the cubic axes, by excluding the related C2[100] symmetry. In tetragonal SrTiO3 the absorption band splits into three components. In the second model the protons vibrate between the O2- and the Sr2+ ions on the face diagonal of the cube, in the last along the edges of the oxygen octahedron between the two nearest-neighbor oxygen ions. The second model can be rejected, because the claimed vertical mirror plane σ(010) is not observed in the Raman spectra. The third model claims no mirror symmetry because of the tilting of the octahedra. In K1-xLixTaO3, x=0.023, a polar tetragonal phase occurs due to the off-center freezing of the LiK ions along the polar axis. Again three subbands of the OH mode occur. But the bands, which reflected the breakdown of the σ(010) symmetry in SrTiO3, do not occur in K1-xLixTaO3. The observed vertical mirror plane σ(010) reflects the nontilted arrangement of the octahedra in K1-xLixTaO3. In TiO2 (rutile) the Raman data reveal a C1h(001) symmetry for the prominent OH stretch mode. This firmly establishes the model, where the protons vibrate in the center of the open channels between the next-nearest O2- ions in the basal plane. In trigonal LiTaO3 the crystal symmetry is so low that hardly any models can be discriminated on the basis of their symmetry, because most of them deal with C1 symmetry. This is detected in the experiment and thus any orientation not along the trigonal axis or the vertical mirror plane is compatible with this result. Still one model proposed for LiNbO3 can be excluded.

  13. A Model of Median Auroral Electron Flux Deduced from Hardy 2008 Model Probability Density Maps

    DTIC Science & Technology

    2013-11-01

    Statistical hemispheric particle precipitation maps were created for a range of different magnetospheric activity levels defined by the Kp index. The... Magnetospheric Electric Fields, J. Geophys. Res., 86, pp. 768-778. Gussenhoven, M. S., D. A. Hardy, and N. Heinemann, (1983), Systematics of the Equatorward

  14. Using social networks to deduce whether residents or dispersers spread parasites in a lizard population.

    PubMed

    Fenner, Aaron L; Godfrey, Stephanie S; Michael Bull, C

    2011-07-01

    1. Heterogeneity of host behaviour can play an important role in the spread of parasites and pathogens around wildlife populations. Social networks have previously been suggested to represent transmission pathways within a population, but where the dynamics of host-parasite interactions are difficult to observe, networks may also be used to provide insights into transmission processes. 2. Pygmy bluetongue lizards, Tiliqua adelaidensis, occupy individual territories, live exclusively in burrows constructed by spiders in Australian native grasslands and are hosts to a tick, Bothriocroton hydrosauri, and a nematode, Pharyngodon wandillahensis. 3. On five monthly occasions, the locations of all individual lizards in three study plots were used to construct weighted, undirected networks based on proximity of adjacent burrows. 4. The networks were used to explore alternative hypotheses about the spread of each parasite through the population: that stable population members that remained in the same burrow over the study period played a major role in influencing the pattern of infection or that dispersing individuals played a more significant role. 5. For ticks, host individuals that were infected were more connected in the network than uninfected hosts and this relationship remained significant for connections to residents in the population, but not for connections to dispersers. 6. For nematodes, infected and uninfected hosts did not differ in their overall strength of connection in the network, but infected hosts were more connected to dispersers than were uninfected hosts, suggesting that lizards moving across the population are the major agents for the transmission of nematodes. 7. This study shows how network analyses can provide new insights into alternative pathways of parasite spread in wildlife populations, where it is difficult to make direct observations of transmission-related behaviours. © 2011 The Authors. Journal of Animal Ecology © 2011 British Ecological Society.

  15. Seismic structure off the Kii Peninsula, Japan, deduced from passive- and active-source seismographic data

    NASA Astrophysics Data System (ADS)

    Yamamoto, Yojiro; Takahashi, Tsutomu; Kaiho, Yuka; Obana, Koichiro; Nakanishi, Ayako; Kodaira, Shuichi; Kaneda, Yoshiyuki

    2017-03-01

    We conduct seismic tomography to model subsurface seismicity between 2010 and 2012 and structural heterogeneity off the Kii Peninsula, southwestern Japan, and to investigate their relationships with segmentation of the Nankai and Tonankai seismogenic zones of the Nankai Trough. In order to constrain both the shallow and deep structure of the offshore seismogenic segments, we use both active- and passive-source data recorded by both ocean-bottom seismometers and land seismic stations. The relocated microearthquakes indicate a lack of seismic activity in the Tonankai seismogenic segment off Kumano, whereas there was active intraslab seismicity in the Kii Channel area of the Nankai seismogenic segment. Based on comparisons among the distribution of seismicity, age, and spreading rate of the subducting Philippine Sea plate, and the slip-deficit distribution, we conclude that seismicity in the subducting slab under the Kii Channel region nucleated from structures in the Philippine Sea slab that pre-date subduction and that fluids released by dehydration are related to decreased interplate coupling of these intraslab earthquakes. Our velocity model clearly shows the areal extent of two key structures reported in previous 2-D active-source surveys: a high-velocity zone beneath Cape Shionomisaki and a subducted seamount off Cape Muroto, both of which are roughly circular and of 15-20 km radius. The epicenters of the 1944 Tonankai and 1946 Nankai earthquakes are near the edge of the high-velocity body beneath Cape Shionomisaki, suggesting that this anomalous structure is related to the nucleation of these two earthquakes. We identify several other high- and low-velocity zones immediately above the plate boundary in the Tonankai and Nankai seismogenic segments. In comparison with the slip-deficit model, some of the low-velocity zones appear to correspond to an area of strong coupling. Our observations suggest that, unlike the Japan Trench subduction zone, in our study area there is not a simple correspondence between areas of large coseismic slip or strong interplate coupling and areas of high velocity in the overriding plate.

  16. Holocene oceanographic and climatic variability of the Vega Drift deduced through foraminiferal interpretation

    USGS Publications Warehouse

    Szymcek, Phillip; Ishman, Scott E.; Domack, Eugene W.; Leventer, Amy

    2007-01-01

    fusiformis assemblages. Most agglutinated forms tend to decrease downcore, and comparisons to modern analogues imply post-depositional disintegration, while calcareous taxa indicate non-corrosive bottom waters. The lower to middle Holocene Vega Drift sediments are characterized by the calcareous S. fusiformis assemblage and glacial plume sediments. This assemblage is characterized by calcareous forms including Globocassidulina biora, G. subglobosa, and Nonionella iridea. The planktic species Neogloboquadrina pachyderma is associated with the S. fusiformis assemblage. The S. fusiformis assemblage is faunally similar to assemblages described in fjords of the western Antarctic Peninsula and indicates non-corrosive bottom water. Sediments of the mid to upper Holocene interval are characterized by the T. wiesneri and M. arenacea assemblages and indicate the presence of Hyper Saline Shelf Water. These assemblages are similar to modern assemblages directly to the south in the Prince Gustav Channel. The upper Holocene is marked by several small intervals with taxonomic characteristics similar to the S. fusiformis assemblage, indicating periodic introduction of non-corrosive bottom water to the Vega Drift

  17. Diurnal Variation of Hydration from Lunar Soil Deduced from Albedo Proton Radiation

    NASA Astrophysics Data System (ADS)

    Schwadron, N. A.; Wilson, J. K.; Looper, M.; Jordan, A. P.; Spence, H. E.; Farrell, W. M.; Petro, N.; Stubbs, T. J.; Pieters, C.; Townsend, L. W.

    2016-11-01

    We show first results from CRaTER comparing horizon to nadir observations in morning and evening sectors. Significant differences in the ratio of horizon to nadir protons suggests migration of volatiles may cause observed variations.

  18. Growth patterns of fossil vertebrates as deduced from bone microstructure: case studies from India.

    PubMed

    Ray, S; Mukherjee, D; Bandyopadhyay, S

    2009-11-01

    Bone microstructure is affected by ontogeny, phylogeny, biomechanics and environments. These aspects of life history of an extinct animal, especially its growth patterns, may be assessed as fossil bone generally maintains its histological integrity. Recent studies on the bone histology of fossil vertebrates from India encompass different types of temnospondyls and dicynodonts from different Permian and Triassic horizons. The examined taxa show that they had distinct bone histology and varied growth patterns. The Early Triassic trematosaurids had an overall fast growth, which contrasts with that of the Middle and Late Triassic temnospondyl taxa examined. The dicynodonts on the other hand, were characterized by an overall fast growth with periodic interruptions, variable growth rates dependent on ontogeny and indeterminate growth strategy. A comparative study encompassing several neotherapsid genera including the dicynodonts shows signifi cant evolutionary trends towards determinate growth strategy and reduced developmental plasticity.

  19. Crustal deformations associated with the great Sumatra-Andaman earthquake deduced from continuous GPS observation

    NASA Astrophysics Data System (ADS)

    Hashimoto, Manabu; Choosakul, Nithiwatthn; Hashizume, Michio; Takemoto, Shuzo; Takiguchi, Hiroshi; Fukuda, Yoichi; Fujimori, Kunio

    2006-02-01

    We analyzed continuous GPS data from more than 20 sites in Asia, Australia and islands in Indian Ocean in order to detect crustal deformations associated with the Sumatra-Andaman earthquake of December 26, 2004. Coseismic steps can be recognized at sites about 3,000 km away from the epicenter such as Kunming in south China, Quezon in Philippines, and Diego Garcia Island in central Indian Ocean. The largest displacement of about 26 cm is found at Phuket in Thailand about 600 km away from the epicenter, about twice as large as that at Sampari, the nearest site in northern Sumatra. These observations suggest that as large slip as 14 m occurred beneath the Nicobar Islands. Large postseismic displacements are observed at Phuket and Sampari after the mainshock, but the former is three times larger than the latter. This suggests that the spatial distribution of afterslip is different from the coseismic slip distribution. The temporal variation of postseismic displacements can be explained by a logarithmic function derived from rate-state dependent friction law with short characteristic time. The area where coseismic displacements from the Nias earthquake of March 28, 2005 are detected is much smaller than that from the December mainshock, but displacement at Sampari is larger than that during the mainshock. These displacements suggest less than 4 m slip on a shallow dipping thrust fault and resultant moment release is smaller than that estimated from seismological data. Finally, total moment released by afterslip amounts to 3.83 × 1022 Nm which is equivalent to Mw 8.99 for about five months, including the afterslip for the Nias earthquake.

  20. Methanogenic capabilities of ANME-archaea deduced from (13) C-labelling approaches.

    PubMed

    Bertram, Sebastian; Blumenberg, Martin; Michaelis, Walter; Siegert, Michael; Krüger, Martin; Seifert, Richard

    2013-08-01

    Anaerobic methanotrophic archaea (ANME) are ubiquitous in marine sediments where sulfate dependent anaerobic oxidation of methane (AOM) occurs. Despite considerable progress in the understanding of AOM, physiological details are still widely unresolved. We investigated two distinct microbial mat samples from the Black Sea that were dominated by either ANME-1 or ANME-2. The (13) C lipid stable isotope probing (SIP) method using labelled substances, namely methane, bicarbonate, acetate, and methanol, was applied, and the substrate-dependent methanogenic capabilities were tested. Our data provide strong evidence for a versatile physiology of both, ANME-1 and ANME-2. Considerable methane production rates (MPRs) from CO2 -reduction were observed, particularly from ANME-2 dominated samples and in the presence of methane, which supports the hypothesis of a co-occurrence of methanotrophy and methanogenesis in the AOM systems (AOM/MPR up to 2:1). The experiments also revealed strong methylotrophic capabilities through (13) C-assimilation from labelled methanol, which was independent of the presence of methane. Additionally, high MPRs from methanol were detected in both of the mat samples. As demonstrated by the (13) C-uptake into lipids, ANME-1 was found to thrive also under methane free conditions. Finally, C35 -isoprenoid hydrocarbons were identified as new lipid biomarkers for ANME-1, most likely functioning as a hydrogen sink during methanogenesis.

  1. Sea level change and glacio-hydro isostasy deduced from some Italian marine notches

    NASA Astrophysics Data System (ADS)

    Antonioli, F.; Carulli, G. B.; Marocco, R.

    2003-04-01

    Sea level change along the Italian coast is the sum of eustatic, glacio-hydro-isostatic, and tectonic factors (Lambeck et al.,submitted 2003). Marine notches are frequently found in limestones lithologies. A marine notch is normally carved in 2-5 centuries and this range depends upon the lithology. The use of notches as sea level marker in a low-tide seas as Mediterranean, is crucial to study the sea level change. In many Italian tectonically active coastal zone uplifting (eastern Sicily) o subsiding (Trieste, north Adriatic sea) the Present day marine notches are lacking because the tectonic ratea are faster than the possibility of carving. A submerged marine notch was discovered at Trieste at an altitude (tidally corrected) of -1.9 m; a similar notch, at an altitude of -0.6, was measured by Fouache et al., (2000) along Croatian coast, but while at Trieste the Present day marine notch is lacking, along Croatian coast, instead, this notch is well carved. This should result from complex equilibrium between eustatic sea level, hydro-glacio isostasy and tectonic that must be different between Trieste and Croatian coast. The deeper submerged marine notch on limestones of Trieste is suitable with an Plio-quaternary tilting of the Carso plateau, gently dipping to NW, as illustrated by other observations by Carulli et al.,(1980). This fact may be related also to the increasing weight of the external fronts of the South-alpine chain and to the External Dinarides one. Both, characterized by strong seismic activity and consequent neotectonics effects, are rising and advancing not far from Trieste. In a tectonically stable zone as Gaeta (Central Tyrrhenian sea) the submerged cliff immediately belove the present day marine notch was monitored (Antonioli, 1989) and was discovered a smoothed morphology similar to a very large marine notch. Such composite morphology made off from two different notches (in 4-5 meters of submerged cliff) is present also as fossil and related to previous higstands (MIS 5.5 and 9) in numerous sites on Tyrrhenian sea: Sardinia, Caccia Cape and Orosei gulf (NW and E coast); Gaeta and Terracina, Latium, (central Tyrrenian sea); Campanella Promontory in Campania, and S.Vito lo Capo in Sicily (S Tyrrenian sea). All this sites show a tidal marine notch (younger) and an older smoothed notch with Lithophaga holes. It is reasonable to presume that these double marine notches (that was found only in stable coasts) should be due, the older and smooted to glacio-hydro isostatic crust movements and the younger (tidal) to the quasi-stillstand of eustatic and isostatic movements of the Present. The measurements and interpetation of these marine notches could be crucial to understand the present and future eustatic-isostatic-tectonic coastal movements.

  2. Postglacial species displacement in Triturus newts deduced from asymmetrically introgressed mitochondrial DNA and ecological niche models

    PubMed Central

    2012-01-01

    Background If the geographical displacement of one species by another is accompanied by hybridization, mitochondrial DNA can introgress asymmetrically, from the outcompeted species into the invading species, over a large area. We explore this phenomenon using the two parapatric crested newt species, Triturus macedonicus and T. karelinii, distributed on the Balkan Peninsula in south-eastern Europe, as a model. Results We first delimit a ca. 54,000 km2 area in which T. macedonicus contains T. karelinii mitochondrial DNA. This introgression zone bisects the range of T. karelinii, cutting off a T. karelinii enclave. The high similarity of introgressed mitochondrial DNA haplotypes with those found in T. karelinii suggests a recent transfer across the species boundary. We then use ecological niche modeling to explore habitat suitability of the location of the present day introgression zone under current, mid-Holocene and Last Glacial Maximum conditions. This area was inhospitable during the Last Glacial Maximum for both species, but would have been habitable at the mid-Holocene. Since the mid-Holocene, habitat suitability generally increased for T. macedonicus, whereas it decreased for T. karelinii. Conclusion The presence of a T. karelinii enclave suggests that T. karelinii was the first to colonize the area where the present day introgression zone is positioned after the Last Glacial Maximum. Subsequently, we propose T. karelinii was outcompeted by T. macedonicus, which captured T. karelinii mitochondrial DNA via introgressive hybridization in the process. Ecological niche modeling suggests that this replacement was likely facilitated by a shift in climate since the mid-Holocene. We suggest that the northwestern part of the current introgression zone was probably never inhabited by T. karelinii itself, and that T. karelinii mitochondrial DNA spread there through T. macedonicus exclusively. Considering the spatial distribution of the introgressed mitochondrial DNA and the signal derived from ecological niche modeling, we do not favor the hypothesis that foreign mitochondrial DNA was pulled into the T. macedonicus range by natural selection. PMID:22935041

  3. Aromatic hydrocarbon emissions in the United States deduced from tall tower measurements

    NASA Astrophysics Data System (ADS)

    Hu, L.; Millet, D. B.; Griffis, T. J.; Tessum, C.; Travis, K.

    2013-12-01

    Aromatic compounds including benzene (C6H6), toluene (C7H8), and xylenes (C8H10) are important anthropogenic precursors of secondary organic aerosol and ground-level ozone. In addition, benzene has been classified as a Group 1 carcinogen by the International Agency for Research on Cancer (IARC). However, their emissions remain poorly constrained. Here we present a full year (2011) of continuous aromatic hydrocarbon measurements by PTR-MS at the University of Minnesota tall tower Tracer Gas Observatory (KCMP tall tower, 244 m a.g.l, 44.689°N, 93.073°W). We interpret the tall tower data with a 0.5° ×0.667° GEOS-Chem nested grid simulation to constrain US sources of benzene, toluene, and xylenes. The tall tower observations reveal a clear high bias in the model, with model: measurement slopes of 1.8, 5.3, and 2.9 for benzene, toluene, and xylenes, respectively. The high bias reflects an overestimate of aromatic hydrocarbon emissions in the model, which are based on the RETRO emission Inventory. In this work, we employ a Bayesian inversion to interpret the KCMP tall tower measurements in terms of quantitative constraints on US aromatic sources and the importance of sectors such as on-road transportation, waste treatment and disposal, solvent use, etc. We discuss our findings relative to other emission estimates (e.g., the EPA NEI) and in terms of their implications for air quality modeling.

  4. Fungal diversity from various marine habitats deduced through culture-independent studies.

    PubMed

    Manohar, Cathrine Sumathi; Raghukumar, Chandralata

    2013-04-01

    Studies on the molecular diversity of the micro-eukaryotic community have shown that fungi occupy a central position in a large number of marine habitats. Environmental surveys using molecular tools have shown the presence of fungi from a large number of marine habitats such as deep-sea habitats, pelagic waters, coastal regions, hydrothermal vent ecosystem, anoxic habitats, and ice-cold regions. This is of interest to a variety of research disciplines like ecology, evolution, biogeochemistry, and biotechnology. In this review, we have summarized how molecular tools have helped to broaden our understanding of the fungal diversity in various marine habitats. Majority of the environmental phylotypes could be grouped as novel clades within Ascomycota, Basidiomycota, and Chytridiomycota or as basal fungal lineages. Deep-branching novel environmental clusters could be grouped within Ascomycota as the Pezizomycotina clone group, deep-sea fungal group-I, and soil clone group-I, within Basidiomycota as the hydrothermal and/or anaerobic fungal group, and within Chytridiomycota as Cryptomycota or the Rozella clade. However, a basal true marine environmental cluster is still to be identified as most of the clusters include representatives from terrestrial regions. The challenge for future research is to explore the true marine fungi using molecular techniques. © 2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

  5. Band gap narrowing and doping level of heavily doped Germanium nanocrystals deduced from photoconductivity studies

    NASA Astrophysics Data System (ADS)

    Lambert, Y.; Gao, Y.; Pi, X. D.; Grandidier, B.; Stiévenard, D.

    2017-09-01

    We investigate the photoconductivity of a n+-ZnO/n-Ge NCs/p+-GaAs junction where the active layer consists of heavily n-doped Ge NCs synthesized in the gas phase. Measurement of a significant current at energies smaller than the band gap of GaAs demonstrates the photogeneration of charge carriers by the Ge NCs. From the correlation of the NC size with the absorption threshold, a narrowing of the direct band gap in the Ge NC thin film is obtained and attributed to the heavy doping of the Ge NCs. A remarkably high electrical activation of 15% is found for the incorporated P impurities in the NCs.

  6. Using Measurements of Fill Factor at High Irradiance to Deduce Heterobarrier Band Offsets: Preprint

    SciTech Connect

    Olson, J. M.; Steiner, M. A.; Kanevce, A.

    2011-07-01

    Using a 2D device simulation tool, we examine the high irradiance behavior of a single junction, GaAs concentrator cell as a function of the doping in the back surface confinement layer. The confinement layer is designed to be a barrier for both holes and electrons in the base of the solar cell. For a p-type base we show that the FF of the cell at high concentrations is a strong function of both the magnitude of the valence band offset and the doping level in the barrier. In short, for a given valence band offset (VBO), there is a critical barrier doping, below which the FF drops rapidly with lower doping. This behavior is confirmed experimentally for a GaInP/GaAs double heterostructure solar cell where the critical doping concentration (at 500 suns) in the back surface confinement layer is ~1e18 cm-3 for a VBO of 300 meV.

  7. High energy runaway electron transport deduced from photonuclear activation of the PLT limiter

    SciTech Connect

    Barnes, C.W.; Stavely, J.M. Jr.; Strachan, J.D.

    1981-07-01

    In large tokamaks, runaway electrons may be accelerated up to energies over 20 MeV by the toroidal electric field. When these particles leave the plasma, they impact the material limiters and emit bremsstrahlung hard x-rays. This bremsstrahlung may cause photonuclear reactions in the limiter, leaving radioisotopes behind. Both the amount of activation and its spatial distribution on the limiter provide information on the confinement of high energy electrons. The stainless steel poloidal ring limiter from the Princeton Large Torus has been analyzed and six observable photonuclear reactions with energy thresholds have been found for the reactions varying from 8 to 23 MeV. The amount of activation from each reaction determines the average number of runaway electrons above each activation threshold energy. The inferred runaway electron population decreases exponentially with energy as exp (-E/3.2 MeV) implying an energetic runaway electron confinement time of 50 to 80 msec. The poloidal variation of the activation can be described by a 0.02 cm scrape-off layer step size which implies about a 90 msec confinement time.

  8. Millennial-scale hard rock erosion rates deduced from luminescence-depth profiles

    NASA Astrophysics Data System (ADS)

    Sohbati, R.; Liu, J.; Murray, A. S.; Jain, M.; Pederson, J. L.; Guralnik, B.; Egholm, D. L.; Gupta, S.

    2015-12-01

    Optically stimulated luminescence (OSL) is a well-established Quaternary dating method that is conventionally used to determine the time when sedimentary grains were last exposed to daylight. Recently, a very different approach to this concept has helped develop a new technique to estimate the length of time a rock surface was exposed to daylight. When a rock surface is first exposed to daylight the charge population (and so the latent luminescence signal) trapped in its constituent minerals (e.g. quartz and feldspar) starts to decrease. This charge had accumulated due to previous exposure to natural ionizing radiation. As the surface is exposed to light for longer periods, the latent luminescence signal is reduced farther into the rock. In a rock surface which has been exposed to light for a prolonged period (decades to millennia), the remaining luminescence will be zero (fully bleached) at the surface and then increase, initially exponentially, before approaching saturation at a depth where charge detrapping due to light penetration is negligible compared to the rate of charge trapping due to the environmental dose rate. By modelling the characteristic shape of luminescence resetting with depth into rock surfaces, Sohbati et al. (2012) proposed a new surface-exposure dating technique based on OSL. Here we further develop the current model to include the effect of erosion rate on luminescence-depth profiles. By fitting the model to local known-age calibration samples, we first determine the site-specific resetting rates of the luminescence signal at rock surfaces. We then use the calibration values in a numerical model to derive the steady-state erosion rate for rocks of different mineralogy and different geological settings. The preliminary erosion rates obtained from glacial and landslide granite boulders from the Chinese Pamir Plateau are ~1 mm.ka-1, whereas active streambeds of Permian sandstone in the Grabens district of Canyonlands National Park, Utah, are eroding at rates of >10 mm.ka-1, consistent with the existing denudation rates derived from cosmogenic nuclides.

  9. Deep ocean nutrients during the Last Glacial Maximum deduced from sponge silicon isotopic compositions

    NASA Astrophysics Data System (ADS)

    Hendry, Katharine R.; Georg, R. Bastian; Rickaby, Rosalind E. M.; Robinson, Laura F.; Halliday, Alex N.

    2010-04-01

    The relative importance of biological and physical processes within the Southern Ocean for the storage of carbon and atmospheric pCO 2 on glacial-interglacial timescales remains uncertain. Understanding the impact of surface biological production on carbon export in the past relies on the reconstruction of the nutrient supply from upwelling deep waters. In particular, the upwelling of silicic acid (Si(OH) 4) is tightly coupled to carbon export in the Southern Ocean via diatom productivity. Here, we address how changes in deep water Si(OH) 4 concentrations can be reconstructed using the silicon isotopic composition of deep-sea sponges. We report δ30Si of modern deep-sea sponge spicules and show that they reflect seawater Si(OH) 4 concentration. The fractionation factor of sponge δ30Si compared to seawater δ30Si shows a positive relationship with Si(OH) 4, which may be a growth rate effect. Application of this proxy in two down-core records from the Scotia Sea reveals that Si(OH) 4 concentrations in the deep Southern Ocean during the Last Glacial Maximum (LGM) were no different than today. Our result does not support a coupling of carbon and nutrient build up in an isolated deep ocean reservoir during the LGM. Our data, combined with records of stable isotopes from diatoms, are only consistent with enhanced LGM Southern Ocean nutrient utilization if there was also a concurrent reduction in diatom silicification or a shift from siliceous to organic-walled phytoplankton.

  10. Evolution of geothermal fluids deduced from chemistry plots: Yellowstone National Park (U.S.A.)

    USGS Publications Warehouse

    Mazor, E.; Thompson, J.M.

    1982-01-01

    Large amounts of chemical data, obtained in geothermal fields, may readily be sorted-out by the aid of a simple set of graphs that provide a clear over-all picture and facilitate the understanding of geochemical processes taking place. As a case study, data from several hundred samples of the thermal springs at the well-known Yellowstone National Park are discussed. The pattern obtained seems to indicate: (1) geochemical similarity between the spring groups of Heart Lake, Shoshone, Upper, Midway, Lower and Norris Geyser Basins, i.e., a geochemical uniformity of major spring groups located over 40 km apart; (2) these groups may be described as originating from a common fluid, most resembling the composition of Norris waters, accompanied by CO2, and other volatiles, that react with igneous rocks, forming local variations; (3) the secondary reactions occur at (medium) depth, before the ascent to the surface; (4) extensive concentration-dilution processes occur during the ascent to the surface. The water of the Mammoth group may be described as originating from the same Norris-like fluid that has been diluted (low Na and Cl contents) and intensively reacted with carbonaceous rocks, thus gaining in Ca, Mg, SO4, and HCO3. ?? 1982.

  11. Observed and modeled global-ocean turbulence regimes as deduced from surface trajectory data

    NASA Astrophysics Data System (ADS)

    Nilsson, Jenny A. U.; Döös, Kristofer; Ruti, Paolo Michele; Artale, Vincenzo; Coward, Andrew; Brodeau, Laurent

    2013-04-01

    This study aims to follow up and remember the work on Lagrangian diffusion undertaken by Volfango Rupolo, who died prematurely nearly three years ago. It was within this field that he achieved the highest of creativity, and his rather recent 2007 work Rupolo (2007) is undoubtedly the fullest and most important example. In that paper, he identified the utility of the relationship between the acceleration and velocity time scales of Lagrangian trajectories, and he separated these trajectories into four homogenous classes according to their correlation and dispersal properties. This classification is better known as 'trajectories taxonomy' and can be used to characterize and separate different turbulence regimes in the global ocean (Rupolo, 2007). He showed that the Lagrangian time scales could be obtained from the inverse use of Lagrangian Stochastic Models, and proposed a screening method for rationalizing the data analysis using the time scale relationships, and successfully applied it to both drifter and Argo-float observations. In the present study, his data analysis methods have been extended to study and evaluate trajectories from both surface drifters and an ocean general circulation model (OGCM) with different grid resolutions. The drifter data can most accurately be described as quasi-Lagrangian trajectories due to the fact that although the buoy is freely drifting in the horizontal plane, it is anchored at 15 m depth by its drogue, and thus the observed trajectories represent a two-dimensional (2D) approximation of the surrounding flow field. The model trajectories were obtained by analytical computations of the particle advection equation, using the Lagrangian open-source software package TRACMASS, in the near-surface velocity fields from three different configurations of the global NEMO Ocean/Sea-Ice general circulation model. In global-scale ocean modeling, compromises are frequently made in terms of grid resolution and time-averaging of the output fields since high-resolution data require considerable amounts of storage space. Here, the implications of such approximations on the modeled velocity fields and, consequently, on the particle dispersion, have been assessed through the validation against observed drifter tracks. This study aims, moreover, to shed some light on the relatively unknown turbulent properties of the near-surface ocean dynamics and its representation in numerical models globally and in a number of key regions. These results could be of interest for other studies within the field of turbulent eddy-diffusion parameterization in ocean models, or ocean circulation studies involving long-term coarse-grid model experiments.

  12. A partially differentiated interior for (1) Ceres deduced from its gravity field and shape

    NASA Astrophysics Data System (ADS)

    Park, R. S.; Konopliv, A. S.; Bills, B. G.; Rambaux, N.; Castillo-Rogez, J. C.; Raymond, C. A.; Vaughan, A. T.; Ermakov, A. I.; Zuber, M. T.; Fu, R. R.; Toplis, M. J.; Russell, C. T.; Nathues, A.; Preusker, F.

    2016-09-01

    Remote observations of the asteroid (1) Ceres from ground- and space-based telescopes have provided its approximate density and shape, leading to a range of models for the interior of Ceres, from homogeneous to fully differentiated. A previously missing parameter that can place a strong constraint on the interior of Ceres is its moment of inertia, which requires the measurement of its gravitational variation together with either precession rate or a validated assumption of hydrostatic equilibrium. However, Earth-based remote observations cannot measure gravity variations and the magnitude of the precession rate is too small to be detected. Here we report gravity and shape measurements of Ceres obtained from the Dawn spacecraft, showing that it is in hydrostatic equilibrium with its inferred normalized mean moment of inertia of 0.37. These data show that Ceres is a partially differentiated body, with a rocky core overlaid by a volatile-rich shell, as predicted in some studies. Furthermore, we show that the gravity signal is strongly suppressed compared to that predicted by the topographic variation. This indicates that Ceres is isostatically compensated, such that topographic highs are supported by displacement of a denser interior. In contrast to the asteroid (4) Vesta, this strong compensation points to the presence of a lower-viscosity layer at depth, probably reflecting a thermal rather than compositional gradient. To further investigate the interior structure, we assume a two-layer model for the interior of Ceres with a core density of 2,460-2,900 kilograms per cubic metre (that is, composed of CI and CM chondrites), which yields an outer-shell thickness of 70-190 kilometres. The density of this outer shell is 1,680-1,950 kilograms per cubic metre, indicating a mixture of volatiles and denser materials such as silicates and salts. Although the gravity and shape data confirm that the interior of Ceres evolved thermally, its partially differentiated interior indicates an evolution more complex than has been envisioned for mid-sized (less than 1,000 kilometres across) ice-rich rocky bodies.

  13. Accretion timescale and impact history of Mars deduced from the isotopic systematics of martian meteorites

    NASA Astrophysics Data System (ADS)

    Borg, Lars E.; Brennecka, Gregory A.; Symes, Steven J. K.

    2016-02-01

    High precision Sm-Nd isotopic analyses have been completed on a suite of 11 martian basaltic meteorites in order to better constrain the age of silicate differentiation on Mars associated with the formation of their mantle sources. These data are used to evaluate the merits and disadvantages of various mathematical approaches that have been employed in previous work on this topic. Ages determined from the Sm-Nd isotopic systematics of individual samples are strongly dependent on the assumed Nd isotopic composition of the bulk planet. This assumption is problematic given differences observed between the Nd isotopic composition of Earth and chondritic meteorites and the fact that these materials are both commonly used to represent bulk planetary Nd isotopic compositions. Ages determined from the slope of 146Sm-142Nd whole rock isochrons are not dependent on the assumed 142Nd/144Nd ratio of the planet, but require the sample suite to be derived from complementary, contemporaneously-formed reservoirs. In this work, we present a mathematical expression that defines the age of formation of the source regions of such a suite of samples that is based solely on the slope of a 143Nd-142Nd whole rock isochron and is also independent of any a priori assumptions regarding the bulk isotopic composition of the planet. This expression is also applicable to mineral isochrons and has been used to successfully calculate 143Nd-142Nd model crystallization ages of early refractory solids as well as lunar samples. This permits ages to be obtained using only Nd isotopic measurements without the need for 147Sm/144Nd isotope dilution determinations. When used in conjunction with high-precision Nd isotopic measurements completed on martian meteorites this expression yields an age of formation of the martian basaltic meteorite source regions of 4504 ± 6 Ma. Because the Sm-Nd model ages for the formation of martian source regions are commonly interpreted to record the age at which large scale mantle reservoirs formed during planetary differentiation associated with magma ocean solidification, the age determined here implies that magma ocean solidification occurred several tens of millions of years after the beginning of the Solar System. Recent thermal models, however, suggest that Mars-sized bodies cool rapidly in less than ∼5 Ma after accretion ceases, even in the presence of a thick atmosphere. Assuming these models are correct, an extended period of accretion is necessary to provide a mechanism to keep portions of the martian mantle partially molten until 4504 Ma. Late accretional heating of Mars could either be associated with protracted accretion occurring at a quasi-steady state or alternatively be associated with a late giant impact. If this scenario is correct, then accretion of Mars-sized bodies takes up to 60 Ma and is likely to be contemporaneous with the core formation and possibly the onset of silicate differentiation. This further challenges the concept that isotopic equilibrium is attained during primordial evolution of planets, and may help to account for geochemical evidence implying addition of material into planetary interiors after core formation was completed.

  14. Structure-property relationships in major ampullate spider silk as deduced from polarized FTIR spectroscopy

    NASA Astrophysics Data System (ADS)

    Papadopoulos, P.; Sölter, J.; Kremer, F.

    2007-10-01

    Polarized Fourier Transform Infrared (FTIR) spectroscopy is employed to study structure-property relationships in major ampullate spider silk being exposed to an external mechanical strain. From the measured infrared dichroism of aminoacid-residue - specific bands the molecular order parameter, the frequency width at half-maximum (FWHM) and the spectral position of the absorption maximum are determined in dependence on the external strain. For the highly ordered alanine-rich β sheets a change in the vibrational potential is found for macroscopic strains as low as a few percent. It can be quantitatively described by a quantum-mechanical approach in which the mechanical strain is treated as a weak external perturbation. The immediate microscopic response to the external field proves that β -sheeted crystals are tightly interconnected by pre-stretched chains as suggested recently (Y. Liu et al., Nat. Mater. 4, 901 (2005)).

  15. Deducing conformational variability of intrinsically disordered proteins from infrared spectroscopy with Bayesian statistics

    PubMed Central

    Sethi, Anurag; Anunciado, Divina; Tian, Jianhui; Vu, Dung M.; Gnanakaran, S.

    2013-01-01

    As it remains practically impossible to generate ergodic ensembles for large intrinsically disordered proteins (IDP) with molecular dynamics (MD) simulations, it becomes critical to compare spectroscopic characteristics of the theoretically generated ensembles to corresponding measurements. We develop a Bayesian framework to infer the ensemble properties of an IDP using a combination of conformations generated by MD simulations and its measured infrared spectrum. We performed 100 different MD simulations totaling more than 10 µs to characterize the conformational ensemble of αsynuclein, a prototypical IDP, in water. These conformations are clustered based on solvent accessibility and helical content. We compute the amide-I band for these clusters and predict the thermodynamic weights of each cluster given the measured amide-I band. Bayesian analysis produces a reproducible and non-redundant set of thermodynamic weights for each cluster, which can then be used to calculate the ensemble properties. In a rigorous validation, these weights reproduce measured chemical shifts. PMID:24187427

  16. Fishing for biodiversity: Novel methanopterin-linked C1 transfergenes deduced from the Sargasso Sea metagenome

    SciTech Connect

    Kalyuzhnaya, Marina G.; Nercessian, Olivier; Lapidus, Alla; Chistoserdova, Ludmila

    2004-07-01

    The recently generated database of microbial genes from anoligotrophic environment populated by a calculated 1,800 of major phylotypes (the Sargasso Sea metagenome) presents a great source for expanding local databases of genes indicative of a specific function. In this paper we analyze the Sargasso Sea metagenome in terms of the presence of methanopterin-linked C1 transfer genes that are signature for methylotrophy. We conclude that more than 10 phylotypes possessing genes of interest are present in this environment, and a few of these are relatively abundant species. The sequences representative of the major phylotypes do not appear to belong to any known microbial group capable of methanopterin-linked C1 transfer. Instead, they separate from all known sequences on phylogenetic trees, pointing towards their affiliation with a novel microbial phylum. These data imply a broader distribution of methanopterin-linked functions in the microbial world than previously known.

  17. Mean age of rifting and volcanism on Venus deduced from impact crater densities

    NASA Astrophysics Data System (ADS)

    Price, Maribeth; Suppe, John

    1994-12-01

    UNLIKE the extensively cratered highlands of the Moon and Mars, the surface of Venus does not preserve a record of heavy bombard-ment from the early history of the Solar System1-3. Those craters that are found on Venus appear to be statistically indistinguishable from a random spatial population and rarely show modification by folds, faults and lava flows1-3. Although the volcanic and tec-tonic history of Venus is still much debated2-5, there is mounting evidence for near-global resurfacing ˜300-500 Myr ago1,2,6. Moreover, it has recently been noted that the density of impact craters on large volcanic structures is less than the average crater density of the planet, suggestive of significant activity after the resurfacing event7. It is not clear, however, whether these features represent late remnants of the global event or continuing volcanism and tectonism of a still active planet. To address this question, we have used the regional variations in crater density to date volcan-oes, rifts and coronae which, based on stratigraphic evidence, clearly post-date the main resurfacing event8-11. The calculated mean ages of 70-125 Myr exclude the possibility that the majority of these features represent the final stages of the global event.

  18. Light elements burning reaction rates at stellar temperatures as deduced by the Trojan Horse measurements

    NASA Astrophysics Data System (ADS)

    Lamia, L.; Spitaleri, C.; La Cognata, M.; Palmerini, S.; Puglia, S. M. R.; Sergi, M. L.

    2015-02-01

    Experimental nuclear astrophysics aims at determining the reaction rates for astrophysically relevant reactions at their Gamow energies. For charged-particle induced reactions, the access to these energies is usually hindered, in direct measurements, by the presence of the Coulomb barrier between the interacting particles or by electron screening effects, which make hard the determination of the bare-nucleus S(E)-factor of interest for astrophysical codes. The use of the Trojan Horse Method (THM) appears as one of the most suitable tools for investigating nuclear processes of interest for astrophysics. Here, in view of the recent TH measurements, the main destruction channels for deuterium (2H ), for the two lithium 6,7Li isotopes, for the 9Be and the one for the two boron 10,11B isotopes will be discussed.

  19. Three-dimensional morphology of equatorial plasma bubbles deduced from measurements onboard CHAMP

    NASA Astrophysics Data System (ADS)

    Park, J.; Lühr, H.; Noja, M.

    2015-01-01

    Total electron content (TEC) between Low-Earth-Orbit (LEO) satellites and the Global Navigation Satellite System (GNSS) satellites can be used to constrain the three-dimensional morphology of equatorial plasma bubbles (EPBs). In this study we investigate TEC measured onboard the Challenging Minisatellite Payload (CHAMP) from 2001 to 2005. We only use TEC data obtained when CHAMP passed through EPBs: that is, when in situ plasma density measurements at CHAMP altitude also show EPB signatures. The observed TEC gradient along the CHAMP track is strongest when the corresponding GNSS satellite is located equatorward and westward of CHAMP with elevation angles of about 40-60°. These elevation and azimuth angles are in agreement with the angles expected from the morphology of the plasma depletion shell proposed by Kil et al.(2009).

  20. Climatological Aspects of Aerosol Physical Characteristics in Tunisia Deduced from Sun Photometric Measurements

    PubMed Central

    Chaâbane, Mabrouk; Azri, Chafai; Medhioub, Khaled

    2012-01-01

    Atmospheric and climatic data measured at Thala site (Tunisia) for a long-time period (1977–2001) are used to analyse the monthly, seasonal, and annual variations of the aerosol optical depth at 1 μm wavelength. We have shown that aerosol and microphysical properties and the dominating aerosol types depend on seasons. A comparison of the seasonal cycle of aerosol optical characteristics at Thala site showed that the contribution of long-range transported particles is expected to be larger in summer as a consequence of the weather stability typical of this season. Also, the winter decrease in atmospheric turbidity may result from increases in relative humidity and decreases in temperature, leading to increased particle size and mass and increased fall and deposition velocities. The spring and autumn weather patterns usually carry fine dust and sand particles for the desert area to Thala region. The annual behaviour of the aerosol optical depth recorded a period of stead increase started in 1986 until 2001. Trends in atmospheric turbidity after 1988 could be explained other ways by the contribution of the eruption of Mount Pinatubo in 1991 and by local or regional changes in climate or in aerosol emissions. PMID:22629150

  1. A model for the quaternary structure of human placental insulin receptor deduced from electron microscopy.

    PubMed Central

    Christiansen, K; Tranum-Jensen, J; Carlsen, J; Vinten, J

    1991-01-01

    Electrophoretically pure and functionally intact human placental insulin receptor was studied by electron microscopy with negative-staining techniques. The quaternary structure of the detergent-solubilized receptor was determined. The receptor had the shape of a letter T approximately 24 nm in height and 18 nm in width with a thickness of the stem and the crossbar of 3-4 nm. No consistent change in ultrastructure of the receptor could be detected after the addition of insulin alone or insulin and Mn2+/Mg2+/ATP. After partial reduction of the alpha 2 beta 2 heterotetrameric receptor into alpha beta heterodimers, the electron micrographs showed a clear reduction in average size of the molecule with disappearance of the T profiles characteristic of the alpha 2 beta 2 heterotetramers. By incubation of the heterodimers in a phosphorylation medium containing insulin, a reassociation to molecules with molecular weights of the alpha 2 beta 2 heterotetramer took place judged from SDS/PAGE. Electron microscopy showed that the molecule formed larger aggregates, and only a few solitary T-shaped copies were seen. Images PMID:1986371

  2. Can reliable values of Young's modulus be deduced from Fisher's (1971) spinning lens measurements?

    PubMed

    Burd, H J; Wilde, G S; Judge, S J

    2006-04-01

    The current textbook view of the causes of presbyopia rests very largely on a series of experiments reported by R.F. Fisher some three decades ago, and in particular on the values of lens Young's modulus inferred from the deformation caused by spinning excised lenses about their optical axis (Fisher 1971) We studied the extent to which inferred values of Young's modulus are influenced by assumptions inherent in the mathematical procedures used by Fisher to interpret the test and we investigated several alternative interpretation methods. The results suggest that modelling assumptions inherent in Fisher's original method may have led to systematic errors in the determination of the Young's modulus of the cortex and nucleus. Fisher's conclusion that the cortex is stiffer than the nucleus, particularly in middle age, may be an artefact associated with these systematic errors. Moreover, none of the models we explored are able to account for Fisher's claim that the removal of the capsule has only a modest effect on the deformations induced in the spinning lens.

  3. Structural Investigations of Afghanistan Deduced from Remote Sensing and Potential Field Data

    NASA Astrophysics Data System (ADS)

    Saibi, Hakim; Azizi, Masood; Mogren, Saad

    2016-08-01

    This study integrates potential gravity and magnetic field data with remotely sensed images and geological data in an effort to understand the subsurface major geological structures in Afghanistan. Integrated analysis of Landsat SRTM data was applied for extraction of geological lineaments. The potential field data were analyzed using gradient interpretation techniques, such as analytic signal (AS), tilt derivative (TDR), horizontal gradient of the tilt derivative (HG-TDR), Euler Deconvolution (ED) and power spectrum methods, and results were correlated with known geological structures. The analysis of remote sensing data and potential field data reveals the regional geological structural characteristics of Afghanistan. The power spectrum analysis of magnetic and gravity data suggests shallow basement rocks at around 1 to 1.5 km depth. The results of TDR of potential field data are in agreement with the location of the major regional fault structures and also the location of the basins and swells, except in the Helmand region (SW Afghanistan) where many high potential field anomalies are observed and attributed to batholiths and near-surface volcanic rocks intrusions. A high-resolution airborne geophysical survey in the data sparse region of eastern Afghanistan is recommended in order to have a complete image of the potential field anomalies.

  4. Low Angle Contact Between the Oaxaca and Juárez Terranes Deduced From Magnetotelluric Data

    NASA Astrophysics Data System (ADS)

    Arzate-Flores, Jorge A.; Molina-Garza, Roberto; Corbo-Camargo, Fernando; Márquez-Ramírez, Víctor

    2016-10-01

    We present the electrical resistivity model along a profile perpendicular to the Middle America trench in southern Mexico that reveals previously unrecognized tectonic features at upper to mid-crustal depths. Our results support the hypotheses that the upper crust of the Oaxaca terrane is a residual ~20 km thick crust composed by an ~10 km thick faulted crustal upper layer and an ~10 km thick hydrated and/or mineralized layer. Oaxaca basement overthrust the younger Juárez (or Cuicateco) terrane. The electrical resistivity model supports the interpretation of a slab subducting at a low angle below Oaxaca. Uplift in the Oaxaca region appears to be related to fault reactivation induced by low angle subduction. In the Juárez terrane, isostatic forces may contribute to uplift because it is largely uncompensated. In the Sierra Madre del Sur, closer to the coast, uplift is facilitated by slab-dehydration driven buoyancy. Both gravity and resistivity models are consistent with a thinned upper crust in the northeast end of the profile.

  5. Properties of nearby interstellar hydrogen deduced from Lyman-alpha sky background measurements

    NASA Technical Reports Server (NTRS)

    Thomas, G. E.

    1972-01-01

    For a sufficiently rapid relative motion of the solar system and the nearby interstellar gas, neutral atoms may be expected to penetrate the heliosphere before becoming ionized. Recent satellite measurements of the Lyman alpha emission above the geocorona indicate such an interstellar wind of neutral hydrogen emerging from the direction of Sagittarius and reaching to within a few astronomical units of the sun. A detailed model of the scattering of solar Lyman alpha from the spatial distribution of neutral hydrogen in interplanetary space is presented. This asymmetric distribution is established by solar wind and solar ultraviolet ionization processes along the trajectories of the incoming hydrogen atoms. The values of the interstellar density, the relative velocity, and the gas temperature are adjusted to agree with the Lyman alpha measurements. The results may be interpreted in terms of two models, the cold model and the hot model of the interstellar gas, depending on whether galactic Lyman alpha emission is present at its maximum allowable value or negligibly small.

  6. Some properties of solar magnetic fields as deduced from NSO/KP data and from calculations

    NASA Astrophysics Data System (ADS)

    Fainshtein, V. G.; Khotilovich, A. V.; Rudenko, G. V.

    2003-09-01

    In this paper we carry out a correlative analysis of the measurements of the photospheric magnetic field B d (the magnetic component along the line of sight) from NSO/KP, and of the calculations of the field components on the visible solar disk from these measurements using Rudenko's (2001) potential field approximation method. The analysis revealed the following features: (1) near the equator the character of variation of langle|B d| rangle(ρ) varies with the range of the values of |B d| used in the analysis (here the brackets langle rangle denote an averaging over time and over the area of the portion of the size 10°×10°, and ρ is the angle between this portion and the central meridian (CM)); (2) generally near the equator there is a relatively small field `radiality': the value of langle|B R| rangle/ langle|B F| rangle near the CM varies over the range 1.25 0.9 and increases from the CM to the limb (here B R and B F are the calculated radial and toroidal field components); (3) the magnetic field measurements near the equator are different for different longitudes; (4) in the region of coronal holes (He i λ10 830 Å) and at the calculated bases of open magnetic tubes (OMT) there is an increased `radiality' of the magnetic field in comparison with solar disk areas near the equator. The largest recorded values of the ratios of area-averaged coronal holes (CH) and OMT of the moduli of the radial and transverse field components were 9.2 in CH, and 4 in OMT.

  7. Cooling rates of mid-ocean ridge lava deduced from clinopyroxene spherulites

    NASA Astrophysics Data System (ADS)

    Gardner, James E.; Befus, Kenneth S.; Miller, Nathan R.; Monecke, Thomas

    2014-08-01

    We present a compositional analysis of spherulites and their surrounding groundmass in basaltic andesite lava samples collected from the Pacific-Antarctic Ridge. The spherulites consist of radiating dendritic branches of clinopyroxene, and contain abundant vesicles that are stretched parallel to the branches. Stretched vesicles require that the spherulites grew when the groundmass was liquid, and thus while it was hotter than its glass transition. Although chemical components incompatible in clinopyroxene should have been enriched in the melt by spherulite growth, no compositional variations occur at a scale larger than the 10 μm-wide slit aperture used for laser ablation ICP-MS. The absence of large-scale gradients implies that the spherulites grew faster than the elements could diffuse ~ 10 μm. Because diffusion is temperature controlled, cooling must have been rapid enough to prevent movement. If the spherulites nucleated when the melt was at between 1380 and 1223 K (i.e., undercooled by 0 to 157 K), then the constant concentrations of Li, Na, and K, the fastest diffusing elements analyzed, require temperature to have cooled faster than 106-7 K min- 1. If, instead, the spherulites nucleated at undercoolings of 357 to 465 K (or as cold as 915 K, equal to the glass transition temperature), then the cooling rate could have been as slow as those inferred for glassy rinds on submarine lavas by relaxation geospeedometry. At all possible cooling rates, however, the spherulites must have grown as fast as ~ 103-5 μm s- 1 to reach their final sizes of 1.4 to 3.2 mm. We speculate that the rapid growth resulted from low kinetic barriers to clinopyroxene growth in the basaltic andesite melt.

  8. Petroleum potentialities of central Tunisia as deduced from identification and characterization of oil source rocks

    SciTech Connect

    Saidi, M.; Acheche, M.H.; Inoubli, H. ); Belayouni, H. )

    1991-08-01

    Many potential oil source rocks occur within the Tunisian stratigraphic column, including Silurian-Devonian shales, Albian and upper Albian-Vraconian carbonates, Cenomanian-Turonian black shales and lower Eocene carbonates. This focuses on the inventory of potential oil source rocks in central Tunisia ranging from middle Jurassic to Turonian. The emphasis is on determining their genetic potential and whether they could have generated oil and gas. Geochemical data obtained from the analysis of at least 2,147 samples show this region to be of significant interest as a petroleum prospective area. The main source rocks identified are Toarcian shales, upper Albian-Vraconian carbonates and Cenomanian-Turonian black shales. They contain predominantly type 2 organic matter (oil and gas prone) and are at the low maturity limit of the oil window. The occurrence of those source rocks close to numerous potential reservoir facies supports the conclusion that central Tunisia is a very interesting area for petroleum exploration.

  9. The age of the Veritas asteroid family deduced by chaotic chronology

    NASA Astrophysics Data System (ADS)

    Milani, Andrea; Farinella, Paolo

    1994-07-01

    ASTEROID families are groups of objects produced in disruptive collisions of a parent body. Although family members are widely dispersed in real space, they cluster in the parameter space defined by their so-called proper elements, and can thus be distinguished from the background asteroid population1-3. For most asteroids, these parameters are very close to being invariants of motion and families are still apparent billions of years after their formation4'5. But these parameters undergo chaotic diffusion, and in some cases the rate of diffusion might be large enough that a family member exits from the region of proper-element space occupied by the family after a characteristic time which is shorter than the lifetime of the Solar System. In this case, the characteristic time should provide an approximate upper bound to the age of the family. Here we use this 'chaotic chronology' method to estimate the lifetime of the unusually compact Veritas family. Calculations of the evolu-tion of the proper elements of the family show that two members (including the largest, 490 Veritas) wander outside the borders of the family on a timescale of about 50 Myr, indicating that the family has an age of less than this.

  10. Simulation of multilane freeway traffic with detailed rules deduced from microscopic driving behavior

    PubMed

    Goldbach; Eidmann; Kittel

    2000-02-01

    A simulation to model traffic on a multilane freeway is introduced starting from microscopic driving rules. The model takes each individual car into account with its individual features and actual situations, so that a distribution of parameters as well as different behaviors can easily be analyzed. Therefore, a detailed study of certain situations, driving tactics, vehicle properties, and their influence on the global traffic flow can be performed. The model is discussed, as are first results such as the influence of driver behavior on the fundamental diagram and, in addition, the dynamics of microscopic, individual quantities like separation and difference in speed between successive cars. It turns out that a hysteresis in the reaction of the driver for speeding up and slowing down plays an important role, and effects macroscopic quantities like the shape of the fundamental diagram, e.g., the metastable behavior around the maximum flow and on the speed of observed jams running backward. Furthermore, microscopic time resolved characteristics are strongly influenced, e.g., oscillations in the distance and relative speed between successive cars.

  11. Enzymatic properties, crystallization, and deduced amino acid sequence of an alkaline endoglucanase from Bacillus circulans.

    PubMed

    Hakamada, Yoshihiro; Endo, Keiji; Takizawa, Shuichi; Kobayashi, Tohru; Shirai, Tsuyoshi; Yamane, Takashi; Ito, Susumu

    2002-04-15

    A high-isoelectric-point (pI), alkaline endo-1,4-beta-glucanase (Egl-257) of Bacillus circulans KSM-N257 was purified to homogeneity and crystallized. The purified enzyme hydrolyzed carboxymethyl cellulose (CMC) with optima of pH 8.5 and 55 degrees C. The molecular mass was 43 kDa, and the pI was pH 9.3. The structural gene contained a single open reading frame of 1221 bp, corresponding to 407 amino acids (aa), including a 30-aa signal peptide (377 aa and 41,680 Da for the mature enzyme). Egl-257 hydrolyzed lichenan and showed 76.3% aa identity to a lichenase from B. circulans WL-12 belonging to glycosyl hydrolase family 8 but did not hydrolyze laminarin, curdran, and xylan at all. This indicates that Egl-257 is a true endo-1,4-beta-glucanase. However, this enzyme was not active on p-nitrophenyl beta-D-cellotrioside and p-nitrophenyl beta-D-cellotetraoside. It was crystallized by the hanging-drop vapor-diffusion method with phosphate plus CdCl(2) as precipitant. Pyramid-like crystals were formed, and they diffracted X-rays beyond 2.2 A resolution. It belongs to the space group P2(1)2(1)2(1) with unit cell parameters of a=62.5 A, b=71.7 A, and c=88.6 A.

  12. Properties of CIRRUS Overlapping Clouds as Deduced from the GOES-12 Imagery Data

    NASA Technical Reports Server (NTRS)

    Chang, Fu-Lung; Minnis, Patrick; Lin, Bing; Sun-Mack, Sunny; Khaiyer, Mandana

    2006-01-01

    Understanding the impact of cirrus clouds on modifying both the solar reflected and terrestrial emitted radiations is crucial for climate studies. Unlike most boundary layer stratus and stratocumulus clouds that have a net cooling effect on the climate, high-level thin cirrus clouds can have a warming effect on our climate. Many research efforts have been devoted to retrieving cirrus cloud properties due to their ubiquitous presence. However, using satellite observations to detect and/or retrieve cirrus cloud properties faces two major challenges. First, they are often semitransparent at visible to infrared wavelengths; and secondly, they often occur over a lower cloud system. The overlapping of high-level cirrus and low-level stratus cloud poses a difficulty in determining the individual cloud top altitudes and optical properties, especially when the signals from cirrus clouds are overwhelmed by the signals of stratus clouds. Moreover, the operational satellite retrieval algorithms, which often assume only single layer cloud in the development of cloud retrieval techniques, cannot resolve the cloud overlapping situation properly. The new geostationary satellites, starting with the Twelfth Geostationary Operational Environmental Satellite (GOES-12), are providing a new suite of imager bands that have replaced the conventional 12-micron channel with a 13.3-micron CO2 absorption channel. The replacement of the 13.3-micron channel allows for the application of a CO2-slicing retrieval technique (Chahine et al. 1974; Smith and Platt 1978), which is one of the important passive satellite methods for remote sensing the altitudes of mid to high-level clouds. Using the CO2- slicing technique is more effective in detecting semitransparent cirrus clouds than using the conventional infrared-window method.

  13. Tracer transport in the stratosphere deduced from satellite data and a two-dimensional model

    SciTech Connect

    Choi, W.K.

    1990-01-01

    The effective transport velocity (e.g., the meridional circulation that can produce the observed seasonal variations in the tracer fields) is estimated from both observed tracer distributions and model simulations in the stratosphere. The equatorial double peak structure is emphasized among other features of tracer mixing ratio fields to validate the transport velocity. Three years of zonally averaged N{sub 2}O and CH{sub 4} data from the SAMS instrument on Nimbus 7 are utilized to investigate the annual and semiannual cycles in long-lived tracer mixing ratios. The annual and semiannual variations are shown to be approximately antisymmetric and symmetric about the equator, respectively. Using the first three components of the cycles to estimate the time tendency, the tracer continuity equation is solved diagnostically to obtain the effective transport velocity. A two dimensional isentropic coordinate model is constructed for further study. The meridional velocities and horizontal diffusion coefficients are calculated from the net radiative heating and the x-momentum equation respectively. The tracer continuity equation is integrated in time for N{sub 2}O by using the diabatic circulation and diffusion coefficients. To intensify the equinoctial subsidence, the climatological zonal mean wind and corresponding temperature fields are modified by using a stronger semiannual oscillation in the equatorial region. Thus, the new vertical velocity fields produced change in the resulting tracer patterns.

  14. Ozone trends deduced from combined Nimbus 7 SBUV and NOAA 11 SBUV/2 data

    NASA Technical Reports Server (NTRS)

    Hollandsworth, S. M.; Mcpeters, R. D.; Flynn, L. E.; Planet, W.; Miller, A. J.; Chandra, S.

    1995-01-01

    The long-term time series of global ozone from the Nimbus-7 Solar Backscatter Ultraviolet instrument (SBUV) (Nov. 1978-June 1990) are extended through June 1994 by using measurements from the NOAA-11 SBUV/2. The data sets are merged based upon comparisons during the 18-month overlap period in which both instruments were operational. During this period, the average offset between the two time series is less than 2% in total ozone, and less than 6% in Umkehr layers 1-10. A linear-regression trend model is applied to the extended time series to calculate updated trends as a function of latitude and altitude. Trends through June 1994 are 1.5-2% per decade less negative than through June 1990 in the tropical middle stratosphere (35-40 km) and in the upper stratosphere (45-50 km) at mid-latitudes. In the lower stratosphere, the trends are nearly 1.5% per decade more negative in the southern hemisphere tropical regions to 25 deg S, but remain relatively unchanged elsewhere. The seasonal structure of the total ozone trends is similar to past trend study results, but the magnitude of the seasonal trend can vary by 2% per decade depending on the length of the time series. Both Total Ozone Mapping Spectrometer (TOMS) (through April 1993) and SBUV total ozone time series show small negative trends in the equatorial region, though they are not statistically at the 2-sigma level.

  15. Seasonal variations of Saturn's auroral acceleration region deduced from spectra of auroral radio emissions

    NASA Astrophysics Data System (ADS)

    Kimura, T.; Lamy, L.; Tao, C.; Badman, S. V.; Cecconi, B.; Zarka, P.; Morioka, A.; Miyoshi, Y.; Kasaba, Y.; Maruno, D.; Fujimoto, M.

    2012-09-01

    Multi-instrumental surveys of Saturn's magnetosphere by Cassini have indicated that auroral radio emissions (Saturnian Kilometric Radiation, SKR), aurorae at UV and IR wavelengths and Energetic Neutral Atoms (ENA) from the inner magnetosphere exhibit periodic behavior at around Saturn's rotational period with the north-south asymmetry and seasonal variations [e.g., Gurnett et al., 2010; Mitchell et al., 2009; Nichols et al., 2010]. These rotationally periodic phenomena are suggestive of distinct magnetosphere-ionosphere coupling current systems, rotating at different periods in the northern and southern hemispheres [e.g., Andrews et al., 2010]. These phenomena suggest that the magnetosphere-ionosphere coupling process and associated energy dissipation process (aurora & SKR) are dynamically dependent on both magnetospheric rotations and long-term conditions of the magnetosphere/ionosphere.

  16. Cirrus properties deduced from CO2 lidar observations of zenith-enhanced backscatter from oriented crystals

    NASA Technical Reports Server (NTRS)

    Eberhard, Wynn L.

    1993-01-01

    Many lidar researchers have occasionally observed zenith-enhanced backscatter (ZEB) from middle and high clouds. The ZEB signature consists of strong backscatter when the lidar is pointed directly at zenith and a dramatic decline in backscatter as the zenith angle dips slightly off zenith. Mirror-like reflection from horizontal facets of oriented crystals (especially plates) is generally accepted as the cause. It was found during a 3-year observation program that approximately 50 percent of ice clouds had ZEB, regardless of cloud height. The orientation of crystals and the ZEB they cause are important to study and understand for several reasons. First, radiative transfer in clouds with oriented crystals is different than if the same particles were randomly oriented. Second, crystal growth depends partly on the orientation of the particles. Third, ZEB measurements may provide useful information about cirrus microphysical and radiative properties. Finally, the remarkable effect of ZEB on lidar signals should be understood in order to properly interpret lidar data.

  17. Anti-inflammatory action of type I interferons deduced from mice expressing interferon beta.

    PubMed

    Boscá, L; Bodelón, O G; Hortelano, S; Casellas, A; Bosch, F

    2000-05-01

    Type I interferons (IFN) are widely used for the therapeutic treatment of viral infections, tumor growth and various chronic diseases such as multiple sclerosis. Antagonism between type I IFNs and IFN-gamma has been described in cells of the immune system, in particular in the activation of macrophages. To study the systemic effects of type I IFNs we used transgenic mice carrying a human IFN-beta (hIFN-beta) gene under the control of the rat insulin I promoter. These animals expressed high levels of hIFN-beta in beta-pancreatic cells, and the ability of the macrophages to respond to pro-inflammatory stimuli was analyzed. Transgenic mice exhibited an increased extravasation of cells to the peritoneal cavity after eliciting with thioglycollate broth. The expression of the inducible form of nitric oxide synthase and cyclooxygenase-2, two enzymes involved in inflammation, was impaired in transgenic animals challenged with lipopolysaccharide and IFN-gamma. Analysis of the mechanisms leading to this attenuated inflammatory response showed a decrease in the serum levels of TNF-alpha and an inhibition of the activation of the transcription factor NF-KB in various tissues. These results indicate that systemic administration of IFN-beta might influence the response to pro-inflammatory stimuli, in particular through the antagonism of IFN-gamma signaling.

  18. Declining moisture availability in late Eocene Antarctica as deduced from Nothofagus sporopollenin δ13C

    NASA Astrophysics Data System (ADS)

    Griener, K. W.; Nelson, D. M.; Warny, S.

    2012-12-01

    Palynological data demonstrate that significant changes in vegetation and climate occurred at the Eocene-Oligocene (E-O) Boundary on the Antarctic Peninsula. These changes include decreases in terrestrial palynomorph abundance and diversity as well as dinoflagellate assemblages that reflect colder sea surface temperatures and increased glaciation (Warny and Askin, 2011). Understanding the factors controlling these changes in climate and vegetation is a topic of great interest. One area of remaining uncertainty is how the hydrologic regime varied during Antarctica's shift from greenhouse to icehouse conditions. For example, estimates of Antarctic precipitation from around the E-O boundary based on plant leaf margins (e.g. Francis et al., 2008), clay mineralogy (e.g. Christian and Kennett, 1997), and models (Thorn and DeConto 2006) are vastly different. We used a moving-wire device interfaced with an isotope-ratio mass spectrometer (Sessions et al., 2005; Nelson et al., 2008) to analyze δ13C of small quantities of Nothofagus sporopollenin extracted from Antarctic Eocene SHALDRIL cores from ~35.9 Mya, just prior to the E-O Boundary (Bohaty et al., 2011). We also analyzed δ13C of modern Nothofagus sporopollenin from herbaria specimens and related these results to historical climate data. Our modern data show that carbon isotope discrimination (Δ) of Nothofagus sporopollenin is positively correlated with mean annual and growing-season precipitation, consistent with prior studies that demonstrate a strong relationship between Δ and water availability in C3 plants. Eocene Nothofagus Δ values progressively decreased through time, implying a decline in moisture availability. There is a close correlation between Nothofagus palynomorph abundance (Warny and Askin, 2011) and Δ, indicating that Nothofagus abundance declined in response to decreasing moisture availability. We consider changes in sea surface temperatures as well as increased glaciation as possible causes behind these changes in aridity.

  19. Cold Chemistry In TMC-CP: Temperature Deduced From HC9N Line Intensities

    NASA Astrophysics Data System (ADS)

    Moats, S. J.; Langston, Glen; Soehl, D. C.

    2009-01-01

    We present a model for the temperature of the Taurus Molecular Cloud (TMC) Cyanopolyene Peak (CP). This model is based on a fit to molecular line observations with the Robert C. Byrd Green Bank Telescope. We find this region to be very cold, with temperature of approximately 3 Kelvin. The TMC-CP region is of great interest because of its known high abundance of large organic molecules. Large molecular structures have been observed within TMC-1 by various authors (Pratrap et al 1997, Dickens et al 2001, and Turner et al 2005). The detection of large molecules, such as HC5N, HC7N, and HC9N, suggests that smaller amino acids could also form in the interstellar medium. Amino acids are essential to life found on earth, and it is highly possible that early biological molecules had their origins within the molecular clouds of the interstellar medium. We observed a sequence of HC9N and HC7N line transitions, in the frequency range 11 to 15 GHz, to estimate the temperature of the molecular line emission region. We present radio images of the structure of TMC from our search for the molecules HC11N and HC13N. Finally, we present upper limits on the abundance of selected molecules, based on averaging observations of many spectral lines in the manner described by Langston and Turner (2007). The National Radio Astronomy Observatory is a facility of the National Science Foundation operated under cooperative agreement by Associated Universities, Inc.

  20. Dust charging and density conditions deduced from observations of PMWE modulated by artificial electron heating

    NASA Astrophysics Data System (ADS)

    Havnes, O.; La Hoz, C.; Rietveld, M. T.; Kassa, M.; Baroni, G.; Biebricher, A.

    2011-12-01

    We present an analysis of relatively strong Polar Mesospheric Winter Echoes (PMWE) under artificial electron heating that changes the PMWE intensity. A major purpose is to find reliable estimates of the relaxation time of the heater modified PMWE to their undisturbed state during the heater switch-off phase; the implications regarding charge/discharge mechanisms; and to exploit the diagnostic potential of artificial electron heating. The relaxation time is between 60 to 70 s for the regions with strong PMWE layers and substantial electron heating. This short relaxation time, related to the variation of charges on the nanometer dust which most likely is present in PMWE, rules out ion attachment as the mechanism to bring the dust charges to their equilibrium state. Neutral winds, sweeping the heated electrons out of the radar beam, are unlikely to be the cause of the observed relaxation, since this requires winds of around 100 m s-1. The most probable cause is photo detachment by which negatively charged dust can lose excess electrons by photon absorption with energies less than the dust material's work function. By comparing the observed heating with heating model profiles, the electron density at 65 km height must have been of the order of 3 × 109 m-3. This agrees with PMWE occurring mainly during disturbed conditions with high electron densities. Our results also indicate that in the strongest PMWE layers, electron bite-outs exist consistent with the role of charged dust particles in the mechanism of PMWE and implying larger dust densities.

  1. Decadal variability in core surface flows deduced from geomagnetic observatory monthly means

    NASA Astrophysics Data System (ADS)

    Whaler, K. A.; Olsen, N.; Finlay, C. C.

    2016-10-01

    Monthly means of the magnetic field measurements at ground observatories are a key data source for studying temporal changes of the core magnetic field. However, when they are calculated in the usual way, contributions of external (magnetospheric and ionospheric) origin may remain, which make them less favourable for studying the field generated by dynamo action in the core. We remove external field predictions, including a new way of characterizing the magnetospheric ring current, from the data and then calculate revised monthly means using robust methods. The geomagnetic secular variation (SV) is calculated as the first annual differences of these monthly means, which also removes the static crustal field. SV time-series based on revised monthly means are much less scattered than those calculated from ordinary monthly means, and their variances and correlations between components are smaller. On the annual to decadal timescale, the SV is generated primarily by advection in the fluid outer core. We demonstrate the utility of the revised monthly means by calculating models of the core surface advective flow between 1997 and 2013 directly from the SV data. One set of models assumes flow that is constant over three months; such models exhibit large and rapid temporal variations. For models of this type, less complex flows achieve the same fit to the SV derived from revised monthly means than those from ordinary monthly means. However, those obtained from ordinary monthly means are able to follow excursions in SV that are likely to be external field contamination rather than core signals. Having established that we can find models that fit the data adequately, we then assess how much temporal variability is required. Previous studies have suggested that the flow is consistent with torsional oscillations (TO), solid body-like oscillations of fluid on concentric cylinders with axes aligned along the Earth's rotation axis. TO have been proposed to explain decadal timescale changes in the length-of-day. We invert for flow models where the only temporal changes are consistent with TO, but such models have an unacceptably large data misfit. However, if we relax the TO constraint to allow a little more temporal variability, we can fit the data as well as with flows assumed constant over three months, demonstrating that rapid SV changes can be reproduced by rather small flow changes. Although the flow itself changes slowly, its time derivative can be locally (temporally and spatially) large, in particular when and where core surface secular acceleration peaks. Spherical harmonic expansion coefficients of the flows are not well resolved, and many of them are strongly correlated. Averaging functions, a measure of our ability to determine the flow at a given location from the data distribution available, are poor approximations to the ideal, even when centred on points of the core surface below areas of high observatory density. Both resolution and averaging functions are noticeably worse for the toroidal flow component, which dominates the flow, than the poloidal flow component, except around the magnetic equator where averaging functions for both components are poor.

  2. Surface currents in the Caribbean Sea as deduced from lagrangian observations

    SciTech Connect

    Molinari, R.L.; Spillane, M.; Brooks, I.; Atwood, D.; Duckett, C.

    1981-07-20

    The results of a satellite tracked drifting buoy experiment conducted in the Caribbean Sea from October 1975 through June 1976 are presented. The buoy trajectories suggest that the Aves Rise, Beata Ridge, and Nicaraguan Rise are regions where considerable mesoscale variability occurs in the surface flow. The variability is in the form of current deflections; small diameter eddies ranging in size from 20 to 100 km; large amplitude meanders, with amplitudes greater than 150 km; and large diameter eddies, with diameters greater than 200 km which form on and near these rises and ridges. In the central Caribbean, where the topographic relief is not as dramatic, the flow is predominantly zonal. Intensifications in the surface flow occur off the coast of Colombia, south of the Nicaraguan Rise, and south of the Yucatan Strait.

  3. Atomic oxygen on the Venus nightside: Global distribution deduced from airglow mapping

    NASA Astrophysics Data System (ADS)

    Soret, L.; Gérard, J.-C.; Montmessin, F.; Piccioni, G.; Drossart, P.; Bertaux, J.-L.

    2012-02-01

    The Visible and Infra-Red Thermal Imaging Spectrometer (VIRTIS) instrument on board the Venus Express spacecraft has measured the O 2(a 1Δ) nightglow distribution at 1.27 μm in the Venus mesosphere for more than two years. Nadir observations have been used to create a statistical map of the emission on Venus nightside. It appears that the statistical 1.6 MR maximum of the emission is located around the antisolar point. Limb observations provide information on the altitude and on the shape of the emission layer. We combine nadir observations essentially covering the southern hemisphere, corrected for the thermal emission of the lower atmosphere, with limb profiles of the northern hemisphere to generate a global map of the Venus nightside emission at 1.27 μm. Given all the O 2(a 1Δ) intensity profiles, O 2(a 1Δ) and O density profiles have been calculated and three-dimensional maps of metastable molecular and atomic oxygen densities have been generated. This global O density nightside distribution improves that available from the VTS3 model, which was based on measurements made above 145 km. The O 2(a 1Δ) hemispheric average density is 2.1 × 10 9 cm -3, with a maximum value of 6.5 × 10 9 cm -3 at 99.2 km. The O density profiles have been derived from the nightglow data using CO 2 profiles from the empirical VTS3 model or from SPICAV stellar occultations. The O hemispheric average density is 1.9 × 10 11 cm -3 in both cases, with a mean altitude of the peak located at 106.1 km and 103.4 km, respectively. These results tend to confirm the modeled values of 2.8 × 10 11 cm -3 at 104 km and 2.0 × 10 11 cm -3 at 110 km obtained by Brecht et al. [Brecht, A., Bougher, S.W., Gérard, J.-C., Parkinson, C.D., Rafkin, S., Foster, B., 2011a. J. Geophys. Res., in press] and Krasnopolsky [Krasnopolsky, V.A., 2010. Icarus 207, 17-27], respectively. Comparing the oxygen density map derived from the O 2(a 1Δ) nightglow observations, it appears that the morphology is very different and that the densities obtained in this study are about three times higher than those predicted by the VTS3 model.

  4. Topoclimatic features as deduced from remote sensing and their impacts on ecological systems

    NASA Astrophysics Data System (ADS)

    Lensky, Itamar; Dayan, Uri

    2010-05-01

    Several recent studies have shown that global models are not capable to predict accurately the evolution of climate changes and variability on the regional scale. Misinterpretation of the anticipated climate change impacts on plants and animals are caused by failure of models to capture topoclimatic conditions. In order to capture the climate spatial variability induced by topography it is necessary to conduct topoclimatological studies. The steady growth in remote sensing and its capacities to monitor spatial patterns in climate driven by topography seems to be an indispensable tool to fill the spatial gap existing in topoclimatic studies. We analyzed time series of Land Surface Temperature (LST) from MODerate resolution Imaging Spectroradiometer (MODIS) on NASA's TERRA satellite using temporal Fourier analysis for the Eastern Mediterranean (EM). RGB display of the mean, amplitude and phase of the first harmonic (annual) were used to decipher topoclimatic features, e.g. cold patches of few kilometers differing much from their vicinity, driven by local topography. These time series were also used to calculate recent decadal trends on a fine spatial resolution of 1 km. The decadal trend over the EM is of about 0.3oC with very large local variability (-1.5 to 1.5oC). The day LST trends values are larger than those obtained for the night time, implying tendency towards a more continental climate (desertification). The thermal heterogeneity resulting from topographic diversity has strong impacts on ecological systems (e.g. biodiversity) and on agriculture (e.g. pest management, crop production). Examples of such impacts will be shown for present and predicted climate for 20 years over the EM.

  5. Molecular evolution of arthropod color vision deduced from multiple opsin genes of jumping spiders.

    PubMed

    Koyanagi, Mitsumasa; Nagata, Takashi; Katoh, Kazutaka; Yamashita, Shigeki; Tokunaga, Fumio

    2008-02-01

    Among terrestrial animals, only vertebrates and arthropods possess wavelength-discrimination ability, so-called "color vision". For color vision to exist, multiple opsins which encode visual pigments sensitive to different wavelengths of light are required. While the molecular evolution of opsins in vertebrates has been well investigated, that in arthropods remains to be elucidated. This is mainly due to poor information about the opsin genes of non-insect arthropods. To obtain an overview of the evolution of color vision in Arthropoda, we isolated three kinds of opsins, Rh1, Rh2, and Rh3, from two jumping spider species, Hasarius adansoni and Plexippus paykulli. These spiders belong to Chelicerata, one of the most distant groups from Hexapoda (insects), and have color vision as do insects. Phylogenetic analyses of jumping spider opsins revealed a birth and death process of color vision evolution in the arthropod lineage. Phylogenetic positions of jumping spider opsins revealed that at least three opsins had already existed before the Chelicerata-Pancrustacea split. In addition, sequence comparison between jumping spider Rh3 and the shorter wavelength-sensitive opsins of insects predicted that an opsin of the ancestral arthropod had the lysine residue responsible for UV sensitivity. These results strongly suggest that the ancestral arthropod had at least trichromatic vision with a UV pigment and two visible pigments. Thereafter, in each pancrustacean and chelicerate lineage, the opsin repertoire was reconstructed by gene losses, gene duplications, and function-altering amino acid substitutions, leading to evolution of color vision.

  6. High-frequency Earth rotation variations deduced from altimetry-based ocean tides

    NASA Astrophysics Data System (ADS)

    Madzak, Matthias; Schindelegger, Michael; Böhm, Johannes; Bosch, Wolfgang; Hagedoorn, Jan

    2016-11-01

    A model of diurnal and semi-diurnal variations in Earth rotation parameters (ERP) is constructed based on altimetry-measured tidal heights from a multi-mission empirical ocean tide solution. Barotropic currents contributing to relative angular momentum changes are estimated for nine major tides in a global inversion algorithm that solves the two-dimensional momentum equations on a regular 0.5° grid with a heavily weighted continuity constraint. The influence of 19 minor tides is accounted for by linear admittance interpolation of ocean tidal angular momentum, although the assumption of smooth admittance variations with frequency appears to be a doubtful concept for semi-diurnal mass terms in particular. A validation of the newly derived model based on post-fit corrections to polar motion and universal time (Δ UT1) from the analysis of Very Long Baseline Interferometry (VLBI) observations shows a variance reduction for semi-diurnal Δ UT1 residuals that is significant at the 0.05 level with respect to the conventional ERP model. Improvements are also evident for the explicitly modeled K_1, Q_1, and K_2 tides in individual ERP components, but large residuals of more than 15 μ as remain at the principal lunar frequencies of O_1 and M_2. We attribute these shortcomings to uncertainties in the inverted relative angular momentum changes and, to a minor extent, to violation of mass conservation in the empirical ocean tide solution. Further dedicated hydrodynamic modeling efforts of these anomalous constituents are required to meet the accuracy standards of modern space geodesy.

  7. Ionosphere/thermosphere dynamics as deduced from meridional ionosonde chain at low latitudes

    NASA Astrophysics Data System (ADS)

    Maruyama, Takashi; Uemoto, Junpei; Tsugawa, Takuya; Hidekatsu, Jin; Kubota, Minoru; Saito, Susumu

    Multipoint ionosonde observation was conducted to study ionosphere/thermosphere dynamics in Southeast Asia. For this study, data from three ionosonde stations aligned at 100° E were analyzed. Two of them are near the magnetic conjugate points in Northern Thailand and West Sumatra, Indonesia, and the other is near the magnetic equator in the Malay Peninsula; these are three of five SEALION (Southeast Asia low latitude ionospheric network) ionosonde stations. The F-layer critical frequency (foF2), the propagation factor (M3000F2), and the F-layer virtual height (h'F) were scaled from quarter hourly ionograms at the three stations. The ionospheric height is closely related to the thermospheric dynamics (neutral drags) as well as electromagnetic forces (EXB drifts) and chemical processes (productions and losses). Because h'F strongly depends on the chemical process during daytime, it is better to examine the peak height (hmF2) for the discussion of dynamics. hmF2 was determined from M3000F2 using the modified Shimazaki's formula. The peak height obtained by this method is less accurate near the magnetic equator because the electron density vertical profile around the peak is greatly distorted from the parabolic distribution and the formation of an additional layer (sometimes called the F3 layer). The peak heights at the low-latitude conjugate points were compared for the whole day. The height difference between the two stations is a good indicator of the transequatorial neutral wind as the equatorward wind lifts the ionosphere up, while the poleward wind lowers it. The diurnal variation of the transequatorial wind inferred from the height differences is examined for various seasons. We found that there exists a strong terdiurnal component, which was most significant around the March equinox. Also the results were compared with the HWM model wind. The terdiurnal amplitude inferred in this study was larger than the model wind and the phases of the diurnal variation showed a quite large difference between the model and the measurements depending on the season and time. For nighttime, on the other hand, h'F is a good indicator of the ionospheric height near the magnetic equator and at low latitudes because the chemical process becomes less important. Vertical EXB drifts were evaluated from the height variation at the magnetic equator. By the assist of numerical modeling incorporating with the EXB drift, neutral wind effects during nighttime were examined more quantitatively and accurately. The results of the transequatorial wind effects were generally consistent with that obtained by the hmF2 analysis.

  8. Electric Potential Patterns Deduced for the SUNDIAL Period of 23-26 September 1986

    DTIC Science & Technology

    1990-01-01

    present study, the initial Ottawa, Canada 58.5 356.2 Saint Johns, Canada 57.6 29.1 electric potential patterns in AMIE use the four Point Tunguska , USSR...regional changes shown in Figure 4. Figure 5 shows an event where the two-cell convection appears to be distorted by a general clockwise rotation and a

  9. Estimation of Ionospheric Electrodynamic Parameters Using Ionospheric Conductance Deduced from Bremsstrahlung X-Ray Image Data

    DTIC Science & Technology

    1989-02-16

    for average geomagnetic conditions, they may not be adequate in studying individual events , where enhanced conductivities are highly variable and...inversion technique that are caused by the use of statistically-determined conduct- ance models during individual substorm events . In particular, an...numerical modeling by choosing four events , 0825-0840 UT, 1005-1020 UT, 1145-1200 UT and 2340-2355 UT, on July 23, 1983, as examples of the pre-expansion

  10. Shallow structure and recent evolution of the Aegean Sea deduced from the seismic reflection analysis

    SciTech Connect

    Laure, M.; Mascle, J.

    1988-08-01

    Together with the Tyrrhenian Sea, the Aegean Sea represents one of two marine basins still developing as a consequence of the subduction of the African lithosphere beneath Europe. Despite many geophysical similarities with the Tyrrhenian Sea, the Aegean displays a specific structural segmentation characterized by two distinct domains separated by the central Aegean. To the north of the basin, the so-called North Aegean trough likely represents the western marine extension of the transtensive Anatolian transform fault zone. The northern margin of this area contains a series of disconnected, often thickly sedimented small basins that probably initiated during the late Miocene as a consequence of a dominantly north-south extension; typical uppermost Miocene (Messinian) formations can be observed on seismic grounds. To the south, the Cretan Sea shows clear evidence of important distensive events occurring during two main episodes and following two main trends; a dominantly north-south-directed extension is responsibile for most of the structural features detected along both the Cretan and southern Cyclades margins.

  11. Use of Nanostructure-Initiator Mass Spectrometry to Deduce Selectivity of Reaction in Glycoside Hydrolases

    PubMed Central

    Deng, Kai; Takasuka, Taichi E.; Bianchetti, Christopher M.; Bergeman, Lai F.; Adams, Paul D.; Northen, Trent R.; Fox, Brian G.

    2015-01-01

    Chemically synthesized nanostructure-initiator mass spectrometry (NIMS) probes derivatized with tetrasaccharides were used to study the reactivity of representative Clostridium thermocellum β-glucosidase, endoglucanases, and cellobiohydrolase. Diagnostic patterns for reactions of these different classes of enzymes were observed. Results show sequential removal of glucose by the β-glucosidase and a progressive increase in specificity of reaction from endoglucanases to cellobiohydrolase. Time-dependent reactions of these polysaccharide-selective enzymes were modeled by numerical integration, which provides a quantitative basis to make functional distinctions among a continuum of naturally evolved catalytic properties. Consequently, our method, which combines automated protein translation with high-sensitivity and time-dependent detection of multiple products, provides a new approach to annotate glycoside hydrolase phylogenetic trees with functional measurements. PMID:26579511

  12. Storm Induced Changes of the Topside Ionosphere as Deduced from Incoherent Scatter Radars

    DTIC Science & Technology

    1990-01-01

    11484 Sep 18 05 16 to 1q84 Sep1, 9 05.16 UT Millstone Hill Ion teOMPO’dture (Til (K 2 4 6 8 10 12 14 16 18 20 22 24 LT 600 550 (b) j 500 450 350 b B 10 2...l 350 320 L/ 2 4 6 8 10 12 Id 16. )s 20 22 24 -1 1q84 Oct 17 00 08 o 19)84 Oct 18 00 08 UT Arecibo 22 24 2 4 6 8 0 12 14 16 8 20L 600 I (b) 450 400Y...22 24 UT 19B4 Sep iq 00.08 to 1q84 Sop 20 eZ.OB UT Arecibo Ion, tomp.o-atw-. IT1 K 1 22 24 2 4 6 8 10 12 14 16 18 20 LT 550 500J.50 (b) 45 400 350

  13. Une Experience Novatrice en Guinee-Bissau: Les Centres D'education Populaire Integree (CEPI)

    NASA Astrophysics Data System (ADS)

    Colin, Roland

    1980-09-01

    Since Guinea-Bissau attained independence, it has endeavoured to define a new system of education inspired chiefly by the experience gained in the liberated zones during the period of armed struggle. Thus, when primary education was extended by two years (from 4 to 6 years), an experiment with Centres of Integrated Popular Education (CEPI) was launched in 1977 in the region of Tombali in the Balante area. The present article gives an account of this altogether novel experiment. It underlines the basic principles of the CEPI: to involve adults in the elaboration of educational programmes; to introduce an alternation of school (where the children spend four days) with the community (where they spend the three following days); to link education with transformation. First evaluations have shown the interest taken by farmers in this new education system, which has now been extended to other regions of the country. However, major problems continue to arise, especially that of preparing new educators capable of reproducing the original pedagogical approach followed in the experimental zone without distorting it, and the problem of articulation of the CEPI with the rest of the educational system.

  14. Dissecting mesenchymal stem cell movement: migration assays for tracing and deducing cell migration.

    PubMed

    Spaeth, Erika L; Marini, Frank C

    2011-01-01

    Targeted migration is a necessary attribute for any gene delivery vehicle. Mesenchymal stem cells (MSC) have been used as effective delivery vehicles for treatments against cancer, graft versus host disease, -arthritis, multiple sclerosis, and many other diseases. MSC migrate toward sites of inflammation, however, the true migratory mechanism has yet to be elucidated. There are several receptors and respective chemokines known to be involved in the migration of the MSC. Further insight to MSC migration will be revealed both in vivo and in vitro through the application of migration assays from the most simple, to the more technologically demanding.

  15. Inner ear anatomy is a proxy for deducing auditory capability and behaviour in reptiles and birds

    PubMed Central

    Walsh, Stig A.; Barrett, Paul M.; Milner, Angela C.; Manley, Geoffrey; Witmer, Lawrence M.

    2009-01-01

    Inferences of hearing capabilities and audition-related behaviours in extinct reptiles and birds have previously been based on comparing cochlear duct dimensions with those of living species. However, the relationship between inner-ear bony anatomy and hearing ability or vocalization has never been tested rigorously in extant or fossil taxa. Here, micro-computed tomographic analysis is used to investigate whether simple endosseous cochlear duct (ECD) measurements can be fitted to models of hearing sensitivity, vocalization, sociality and environmental preference in 59 extant reptile and bird species, selected based on their vocalization ability. Length, rostrocaudal/mediolateral width and volume measurements were taken from ECD virtual endocasts and scaled to basicranial length. Multiple regression of these data with measures of hearing sensitivity, vocal complexity, sociality and environmental preference recovered positive correlations between ECD length and hearing range/mean frequency, vocal complexity, the behavioural traits of pair bonding and living in large aggregations, and a negative correlation between ECD length/rostrocaudal width and aquatic environments. No other dimensions correlated with these variables. Our results suggest that ECD length can be used to predict mean hearing frequency and range in fossil taxa, and that this measure may also predict vocal complexity and large group sociality given comprehensive datasets. PMID:19141427

  16. Identification of widespread pollution in the Southern Hemisphere deduced from satellite analyses

    NASA Technical Reports Server (NTRS)

    Fishman, J.; Fakhruzzaman, K.; Cros, B.; Nganga, D.

    1991-01-01

    Vertical profiles of ozone obtained from ozonesondes in Brazzaville (Congo) and Ascension Island show that large quantities of tropospheric ozone are present over southern Africa and the adjacent eastern tropical South Atlantic Ocean. The origin of this pollution is widespread biomass burning in Africa. These measurements support satellite-derived tropospheric ozone data that demonstrate that ozone originating from this region is transported throughout most of the Southern Hemisphere. Seasonally high levels of CO2 and methane observed at middle- and high-latitude stations in Africa, Australia, and Antarctica likely reflect the effects of this distant biomass burning. These data suggest that even the most remote regions on this planet may be significantly more polluted than previously believed.

  17. Outbreaks of Yuzu Dieback in Goheung Area: Possible Causes Deduced from Weather Extremes.

    PubMed

    Kim, Kwang-Hyung; Kim, Gyoung Hee; Son, Kyeong In; Koh, Young Jin

    2015-09-01

    Starting in 2012, severe diebacks usually accompanied by abundant gum exudation have occurred on yuzu trees in Goheung-gun, Jeonnam Province, where severely affected trees were occasionally killed. On-farm surveys were conducted at 30 randomly-selected orchards located at Pungyang-myeon, Goheung-gun, and the resulting disease incidences were 18.5% and 39.6% for dieback and gumming symptoms, respectively. Black spots on branches and leaves also appeared on infected trees showing a typical dieback symptom. Morphological and molecular identifications of the isolated fungal organisms from lesions on the symptomatic leaves and branches revealed that they are identical to Phomopsis citri, known to cause gummosis. In order to find the reason for this sudden epidemic, we investigated the weather conditions that are exclusively distinct from previous years, hypothesizing that certain weather extremes might have caused the severe induction of pre-existing disease for yuzu. There were two extreme temperature drops beyond the yuzu's cold hardiness limit right after an abnormally-warm-temperature-rise during the winter of 2011-12, which could cause severe frost damage resulting in mechanical injuries and physiological weakness to the affected trees. Furthermore, there was an increased frequency of strong wind events, seven times in 2012 compared to only a few times in the previous years, that could also lead to extensive injuries on branches. In conclusion, we estimated that the possible damages by severe frost and frequent strong wind events during 2012 could cause the yuzu trees to be vulnerable to subsequent fungal infection by providing physical entries and increasing plant susceptibility to infections.

  18. Outbreaks of Yuzu Dieback in Goheung Area: Possible Causes Deduced from Weather Extremes

    PubMed Central

    Kim, Kwang-Hyung; Kim, Gyoung Hee; Son, Kyeong In; Koh, Young Jin

    2015-01-01

    Starting in 2012, severe diebacks usually accompanied by abundant gum exudation have occurred on yuzu trees in Goheung-gun, Jeonnam Province, where severely affected trees were occasionally killed. On-farm surveys were conducted at 30 randomly-selected orchards located at Pungyang-myeon, Goheung-gun, and the resulting disease incidences were 18.5% and 39.6% for dieback and gumming symptoms, respectively. Black spots on branches and leaves also appeared on infected trees showing a typical dieback symptom. Morphological and molecular identifications of the isolated fungal organisms from lesions on the symptomatic leaves and branches revealed that they are identical to Phomopsis citri, known to cause gummosis. In order to find the reason for this sudden epidemic, we investigated the weather conditions that are exclusively distinct from previous years, hypothesizing that certain weather extremes might have caused the severe induction of pre-existing disease for yuzu. There were two extreme temperature drops beyond the yuzu’s cold hardiness limit right after an abnormally-warm-temperature-rise during the winter of 2011–12, which could cause severe frost damage resulting in mechanical injuries and physiological weakness to the affected trees. Furthermore, there was an increased frequency of strong wind events, seven times in 2012 compared to only a few times in the previous years, that could also lead to extensive injuries on branches. In conclusion, we estimated that the possible damages by severe frost and frequent strong wind events during 2012 could cause the yuzu trees to be vulnerable to subsequent fungal infection by providing physical entries and increasing plant susceptibility to infections. PMID:26361477

  19. Angular size of Coronal Mass Ejections deduced from Energetic Particle Observations .

    NASA Astrophysics Data System (ADS)

    Zimbardo, G.; Pommois, P.; Ippolito, A.; Veltri, P.

    The angular size of coronal mass ejections (CME) moving around the Sun - Earth direction, which are likely to cause space weather disturbances, are not easily determined by direct observations. Here we develop a method to correlate the CME semi size with the observation of solar energetic particles (SEPs), which are often associated with CME and flares. These energetic particles propagate through the interplanetary medium, where relevant levels of magnetic turbulence are found. We study the magnetic connection from the Earth to the solar corona by means of a numerical simulation in which turbulence levels and solar wind velocities obtained from web based data sets are used. The simulation results are compared to solar flare observations contained in the GOES catalogue for the years 1996, 1997, 1998, following solar minimum. For this data set, we find that SEPs can reach the Earth when the difference in the heliographic longitudes of the flare and of the magnetic foot point of the Earth is 25-30 degrees at most. On the other hand, the angular semi width of magnetic field line random walking in the solar wind from the Earth to the solar corona is found to be typically 6-10 degrees. The difference between the two estimates corresponds to a flare-associated CME shock with longitudinal semi size of 20-25 degrees. This estimate is in good agreement with previous mean size estimates from the Solar Maximum Mission coronograph/polarimeter.

  20. Postseismic deformation associated with the 2011 Tohoku Earthquake deduced by means of GPS/Acoustic surveys

    NASA Astrophysics Data System (ADS)

    Tomita, Fumiaki; Kido, Motoyuki; Ohta, Yusaku; Iinuma, Takeshi; Hino, Ryota

    2017-04-01

    Postseismic deformation of the 2011 Tohoku Earthquake has been measured by on- and off-shore geodetic studies (e.g., Ozawa et al., 2012, JGR; Watanabe et al., 2014, GRL), which generally showed trenchward movement in the on-shore area and landward movement in the off-shore, above the coseismic primary rupture area (PRA). These features were roughly modeled as viscoelastic relaxation and local afterslip (e.g., Sun et al., 2014, Nature). However, the previous observations are insufficient to describe whole spatial pattern of the postseismic deformation, especially in the off-shore areas along the Japan Trench. In this study, we show observation results using a wide GPS/acoustic (GPS/A) observation network (Kido et al., 2015, IAG) deployed for spatially constraining the postseismic deformation pattern along the trench. We had conducted repeated campaign surveys from Sep. 2012 to Sep. 2016 at 20 GPS/A sites. GPS/A positioning was performed by means of Kido et al. (2006, EPS). Then, a horizontal postseismic displacement rate at each site is calculated by a weighted robust linear fitting technique. The obtained displacement rates demonstrate clear spatial variation of the postseismic deformation; slight trenchward movement (< 5 cm/yr) in the north region of PRA, significant trenchward movement (5-15 cm/yr) in the south region of PRA, and significant landward movement (10-15 cm/yr) above PRA were shown. To investigate contributed postseismic deformation processes, we compared our results with the displacement rates predicted from an existing viscoelastic relaxation model (VR model, Sun et al., 2014). The observed landward movement can be roughly explained by the VR model, but it cannot be fully accounted, which suggests a contribution of interplate locking causing further landward movement. We expect that the pattern of the observed landward movement has potential for demonstrating the spatial variation of the interpolate locking and for revising the VR model. In the north region of PRA, both of the observed and the VR modeled movement are small; contribution of other processes (e.g., afterslip and interplate locking) might be suggested to be trivial. In the south region of PRA, the observed trenchward movements are clearly larger than ones predicted from the VR model, which would be accounted by afterslip. Meanwhile, the results in the south region of PRA may show temporal decay of afterslip; such a temporal decay has not been detected in the region where viscoelastic relaxation is a dominant deformation process.

  1. Radial profile of the inner heliospheric magnetic field as deduced from Faraday rotation observations

    NASA Astrophysics Data System (ADS)

    Mancuso, S.; Garzelli, M. V.

    2013-05-01

    Faraday rotation measures (RMs) of the polarized emission from extragalactic radio sources occulted by the coronal plasma were used to infer the radial profile of the inner heliospheric magnetic field near the solar minimum. By inverting LASCO/SOHO polarized brightness (pB) data taken during the observations in May 1997, we retrieved the electron density distribution along the lines of sight to the sources, which allowed us to separate the two plasma properties that contribute to the observed RMs. By comparing the observed RM values with those theoretically predicted by a power law model of the radial component of the coronal magnetic field using a best-fitting procedure, we found that the radial component of the inner heliospheric magnetic field can be nicely approximated by a power law of the form Br = 3.76 r-2.29 G in a range of heights from about 5 to 14 R⊙. Finally, our analysis suggests that the radial computation of the potential field source surface model from the Wilcox Solar Observatory is the preferred choice near solar minimum assuming a radial field in the photosphere and a source surface located at Rss = 2.5 R⊙.

  2. Mean shape of interplanetary shocks deduced from in situ observations and its relation with interplanetary CMEs

    NASA Astrophysics Data System (ADS)

    Janvier, M.; Démoulin, P.; Dasso, S.

    2014-05-01

    Context. Shocks are frequently detected by spacecraft in the interplanetary space. However, the in situ data of a shock do not provide direct information on its overall properties even when a following interplanetary coronal mass ejection (ICME) is detected. Aims: The main aim of this study is to constrain the general shape of ICME shocks with a statistical study of shock orientations. Methods: We first associated a set of shocks detected near Earth over 10 years with a sample of ICMEs over the same period. We then analyzed the correlations between shock and ICME parameters and studied the statistical distributions of the local shock normal orientation. Supposing that shocks are uniformly detected all over their surface projected on the 1 AU sphere, we compared the shock normal distribution with synthetic distributions derived from an analytical shock shape model. Inversely, we derived a direct method to compute the typical general shape of ICME shocks by integrating observed distributions of the shock normal. Results: We found very similar properties between shocks with and without an in situ detected ICME, so that most of the shocks detected at 1 AU are ICME-driven even when no ICME is detected. The statistical orientation of shock normals is compatible with a mean shape having a rotation symmetry around the Sun-apex line. The analytically modeled shape captures the main characteristics of the observed shock normal distribution. Next, by directly integrating the observed distribution, we derived the mean shock shape, which is found to be comparable for shocks with and without a detected ICME and weakly affected by the limited statistics of the observed distribution. We finally found a close correspondence between this statistical result and the leading edge of the ICME sheath that is observed with STEREO imagers. Conclusions: We have derived a mean shock shape that only depends on one free parameter. This mean shape can be used in various contexts, such as studies for high-energy particles or space weather forecasts.

  3. Phase lag deduced information in photo-thermal actuation for nano-mechanical systems characterization

    SciTech Connect

    Bijster, R. J. F. Vreugd, J. de; Sadeghian, H.

    2014-08-18

    In photo-thermal actuation, heat is added locally to a micro-cantilever by means of a laser. A fraction of the irradiation is absorbed, yielding thermal stresses and deformations in the structure. Harmonic modulation of the laser power causes the cantilever to oscillate. Moreover, a phase lag is introduced which is very sensitive to the spot location and the cantilever properties. This phase lag is theoretically predicted and experimentally verified. Combined with thermo-mechanical properties of the cantilever and its geometry, the location of the laser spot, the thermal diffusivity, and the layer thicknesses of the cantilever can be extracted.

  4. Characterization of defensin gene from abalone Haliotis discus hannai and its deduced protein

    NASA Astrophysics Data System (ADS)

    Hong, Xuguang; Sun, Xiuqin; Zheng, Minggang; Qu, Lingyun; Zan, Jindong; Zhang, Jinxing

    2008-11-01

    Defensin is one of preserved ancient host defensive materials formed in biological evolution. As a regulator and effector molecule, it is very important in animals’ acquired immune system. This paper reports the defensin gene from the mixed liver and kidney cDNA library of abalone Haliotis discus hannai Ino. Sequence analysis shows that the gene sequence of full-length cDNA encodes 42 mature peptides (including six Cys), molecular weight of 4 323 Da, and pI of 8.02. Amino acid sequence homology analysis shows that the peptides are highly similar (70% in common) to other insects defensin. Because of a typical insect-defensin structural character of mature peptide in the secondary structure, the polypeptide named Haliotis discus defensin (hd-def), a novel of antimicrobial peptides, belongs to insects defensin subfamily. The RT-PCR result of Haliotis discus defensin shows that the gene can be expressed only in the hepatopancreas by Gram-negative and positive bacteria stimulation, which is ascribed to inducible expression. Therefore, it is revealed that the Haliotis discus defensin gene expression was related to the antibacterial infection of Haliotis discus hannai Ino.

  5. Deducing Weathering Processes Using Silicon Isotopes in the Ganges Alluvial Plain, India

    NASA Astrophysics Data System (ADS)

    Frings, P.; De La Rocha, C. L.; Fontorbe, G.; Chakrapani, G.; Clymans, W.; Conley, D. J.

    2014-12-01

    The Ganges Alluvial Plain ('GAP') is the sedimentary infill of the foreland basin created during Himalayan orogeny. Freshly eroded material from the Himalaya and southern cratonic tributaries is deposited into a system with long water-sediment interaction times, creating potential for further generation of river weathering fluxes. To quantify weathering processes in the GAP, 51 sites including all major tributaries were sampled in a September 2013 campaign and analysed for major and minor ions, Ge/Si ratios and δ30Si, δ13C and δ18O. Net dissolved Si (DSi) and major cation yields are 2 to 5 times lower in the GAP than the Himalaya, and at a whole basin scale approximate the global average, indicating that the plain apparently moderates the efficiency of Himalayan weathering rates. Mainstem δ30Si spans 0.81 to 1.93‰ (see figure) and gives the impression of a system buffered to moderate DSi and δ30Si. Ge/Si ratios (µmol/mol) are higher than expected in the Himalaya (>3), reflecting input of Ge-enriched water from hot springs, and decline to ~1.4 in the GAP. For the Himalayan sourced rivers, δ30Si increases with distance from the Himalayan front, and can not be explained entirely by conservative mixing with higher δ30Si peninsular and GAP streams. To a first degree, the δ30Si data suggest incorporation of Si into secondary minerals as the key fractionating process, and that this occurs both in situ during initial weathering and progressively in the GAP. Partitioning of solutes between sources is complicated in the GAP. Consistent with previous work, carbonate weathering dominates the ion fluxes, but with substantial contributions from saline/alkaline soil salts, the chlorination of wastewater and highly variable rainfall chemistry. Due to these contributions, precisely inferring the input from silicate weathering is difficult. We introduce a novel method to infer silicate-weathering rates that exploits the fractionation of Si during clay formation to account for the loss of DSi from solution.

  6. Irradiation history of Itokawa regolith material deduced from noble gases in the Hayabusa samples.

    PubMed

    Nagao, Keisuke; Okazaki, Ryuji; Nakamura, Tomoki; Miura, Yayoi N; Osawa, Takahito; Bajo, Ken-ichi; Matsuda, Shintaro; Ebihara, Mitsuru; Ireland, Trevor R; Kitajima, Fumio; Naraoka, Hiroshi; Noguchi, Takaaki; Tsuchiyama, Akira; Yurimoto, Hisayoshi; Zolensky, Michael E; Uesugi, Masayuki; Shirai, Kei; Abe, Masanao; Yada, Toru; Ishibashi, Yukihiro; Fujimura, Akio; Mukai, Toshifumi; Ueno, Munetaka; Okada, Tatsuaki; Yoshikawa, Makoto; Kawaguchi, Junichiro

    2011-08-26

    Noble gas isotopes were measured in three rocky grains from asteroid Itokawa to elucidate a history of irradiation from cosmic rays and solar wind on its surface. Large amounts of solar helium (He), neon (Ne), and argon (Ar) trapped in various depths in the grains were observed, which can be explained by multiple implantations of solar wind particles into the grains, combined with preferential He loss caused by frictional wear of space-weathered rims on the grains. Short residence time of less than 8 million years was implied for the grains by an estimate on cosmic-ray-produced (21)Ne. Our results suggest that Itokawa is continuously losing its surface materials into space at a rate of tens of centimeters per million years. The lifetime of Itokawa should be much shorter than the age of our solar system.

  7. Distribution of buried hydrothermal alteration deduced from high-resolution magnetic surveys in Yellowstone National Park

    NASA Astrophysics Data System (ADS)

    Bouligand, Claire; Glen, Jonathan M. G.; Blakely, Richard J.

    2014-04-01

    Yellowstone National Park (YNP) displays numerous and extensive hydrothermal features. Although hydrothermal alteration in YNP has been extensively studied, the volume, geometry, and type of rock alteration at depth remain poorly constrained. In this study, we use high-resolution airborne and ground magnetic surveys and measurements of remanent and induced magnetization of field and drill core samples to provide constraints on the geometry of hydrothermal alteration within the subsurface of three thermal areas in YNP (Firehole River, Smoke Jumper Hot Springs, and Norris Geyser Basin). We observe that hydrothermal zones from both liquid- and vapor-dominated systems coincide with magnetic lows observed in aeromagnetic surveys and with a decrease of the amplitude of short-wavelength anomalies seen in ground magnetic surveys. This suggests a strong demagnetization of both the shallow and deep substratum within these areas associated with the removal of magnetic minerals by hydrothermal alteration processes. Such demagnetization is confirmed by measurements of rock samples from hydrothermal areas which display significantly decreased total magnetization. A pronounced negative anomaly is observed over the Lone Star Geyser and suggests a significant demagnetization of the substratum associated with areas displaying large-scale fluid flow. The ground and airborne magnetic surveys are used to evaluate the distribution of magnetization in the subsurface. This study shows that significant demagnetization occurs over a thickness of at least a few hundred meters in hydrothermal areas at YNP and that the maximum degree or maximum thickness of demagnetization correlates closely with the location of hydrothermal activity and mapped alteration.

  8. Use of Ra isotopes to deduce rapid transfer of sediment-derived inputs off Kerguelen

    NASA Astrophysics Data System (ADS)

    Sanial, V.; van Beek, P.; Lansard, B.; Souhaut, M.; Kestenare, E.; d'Ovidio, F.; Zhou, M.; Blain, S.

    2015-03-01

    The Southern Ocean is known to be the largest high-nutrient, low-chlorophyll (HNLC) region of the global ocean due to iron limitation. However, a large phytoplankton bloom develops annually downstream of the Kerguelen Islands, a bloom which is sustained partly by iron released from the sediments deposited onto the shelves. In the framework of the KEOPS-2 project, we used radium isotopes (224Ra, T1/2 = 3.66 d; 223Ra, T1/2 = 11.4 d; 228Ra, T1/2 = 5.75 yr) to provide information on the origin of iron fertilization and on the timescales of the transfer of sediment-derived inputs (including iron and other micronutrients) towards offshore waters. Significant 224Ra and 223Ra activities were found in the near vicinity of the Kerguelen Islands, in agreement with the short half-lives of these isotopes. Significant 224Ra and 223Ra activities were also detected up to 200 km downstream of the islands and more unexpectedly in offshore waters south of the polar front. These observations thus clearly indicate (i) that the sediment-derived inputs are rapidly transferred towards offshore waters (on timescales on the order of several days up to several weeks) and (ii) that the polar front is not a physical barrier for the chemical elements released from the sediments of the Kerguelen Plateau. The Ra data set suggests that iron and other micronutrients released by the shelves of the Kerguelen Islands may contribute to fueling the phytoplankton bloom downstream of the islands, despite the presence of the polar front. However, the heterogeneous distribution of the 224Ra and 223Ra activities in surface waters suggests that this supply across the front is not a continuous process but rather a process that is highly variable in space and time.

  9. Use of Ra isotopes to deduce rapid transfer of sediment-derived inputs off Kerguelen

    NASA Astrophysics Data System (ADS)

    Sanial, V.; van Beek, P.; Lansard, B.; Souhaut, M.; Kestenare, E.; d'Ovidio, F.; Zhou, M.; Blain, S.

    2014-09-01

    The Southern Ocean is known as the largest High-Nutrient, Low-Chlorophyll (HNLC) region of the global ocean due to iron limitation. However, a large phytoplankton bloom develops annually downstream of the Kerguelen Islands, which is sustained partly by iron released from the sediments deposited onto the margins. In the framework of the KEOPS-2 project, we used radium isotopes (224Ra, T1/2 = 3.66 d; 223Ra, T1/2 = 11.4 d; 228Ra, T1/2 = 5.75 yr) to provide information on the origin of iron fertilization and on the timescales of the transfer of sediment-derived inputs (including iron and other micronutrients) towards offshore waters. Significant 223Ra and 223Ra activities were found in the near vicinity of the Kerguelen Islands, in agreement with the short half-lives of these isotopes. Significant 224Ra and 223Ra activities were also detected up to 200 km downstream of the islands and more unexpectedly in offshore waters south of the Polar Front. These observations thus clearly indicate (i) that the sediment-derived inputs are rapidly transferred towards offshore waters (on timescales in the order of several days up to several weeks) and (ii) that the Polar Front is not a physical barrier for the chemical elements released from the sediments of Kerguelen Plateau. The Ra dataset suggests that iron and other micronutrients released by the shallow sediments of the Kerguelen margins may contribute to fuel the phytoplankton bloom downstream of the islands, despite the presence of the Polar Front. However, the heterogeneous distribution of the 224Ra and 223Ra activities in surface waters suggests that this supply across the front is not a continuous process, but rather a process that is highly variable in space and time.

  10. The production rate of cosmogenic 21-Ne in chondrites deduced from 81-Kr measurements

    NASA Technical Reports Server (NTRS)

    Schultz, L.; Freundel, M.

    1986-01-01

    Cosmogenic Ne-21 is used widely to calculate exposure ages of stone meteorites. In order to do so, the production rate P(21) must be known. This rate, however, is dependent on the chemical composition of the meteorite as well as the mass of, and position within, the meteoroid during its exposure to the cosmic radiation. Even for a mean shielding the production rates determined from measurments of different radionuclides vary by a factor of two. A method that can be used to determine exposure ages of meteorites that avoids shielding and chemical composition corrections is the -81-Kr-Kr-method. However, for chondrites, in many cases, the direct determination of production rates for the Kr isotopes is prevented by the trapped gases and the neutron effects on bromine. Therefore, this method was applied to four eucrite falls and then their 81-Kr-83-Kr-ages were compared to their cosmogenic Ne-21 and Ar-38 concentrations. The eucrites Bouvante-le-Haut, Juvinas, Sioux County, and Stannern were chosen for these measurements because of their similar chemical composition regarding the major elements.

  11. Postglacial species displacement in Triturus newts deduced from asymmetrically introgressed mitochondrial DNA and ecological niche models.

    PubMed

    Wielstra, Ben; Arntzen, Jan W

    2012-08-30

    If the geographical displacement of one species by another is accompanied by hybridization, mitochondrial DNA can introgress asymmetrically, from the outcompeted species into the invading species, over a large area. We explore this phenomenon using the two parapatric crested newt species, Triturus macedonicus and T. karelinii, distributed on the Balkan Peninsula in south-eastern Europe, as a model. We first delimit a ca. 54,000 km(2) area in which T. macedonicus contains T. karelinii mitochondrial DNA. This introgression zone bisects the range of T. karelinii, cutting off a T. karelinii enclave. The high similarity of introgressed mitochondrial DNA haplotypes with those found in T. karelinii suggests a recent transfer across the species boundary. We then use ecological niche modeling to explore habitat suitability of the location of the present day introgression zone under current, mid-Holocene and Last Glacial Maximum conditions. This area was inhospitable during the Last Glacial Maximum for both species, but would have been habitable at the mid-Holocene. Since the mid-Holocene, habitat suitability generally increased for T. macedonicus, whereas it decreased for T. karelinii. The presence of a T. karelinii enclave suggests that T. karelinii was the first to colonize the area where the present day introgression zone is positioned after the Last Glacial Maximum. Subsequently, we propose T. karelinii was outcompeted by T. macedonicus, which captured T. karelinii mitochondrial DNA via introgressive hybridization in the process. Ecological niche modeling suggests that this replacement was likely facilitated by a shift in climate since the mid-Holocene. We suggest that the northwestern part of the current introgression zone was probably never inhabited by T. karelinii itself, and that T. karelinii mitochondrial DNA spread there through T. macedonicus exclusively. Considering the spatial distribution of the introgressed mitochondrial DNA and the signal derived from ecological niche modeling, we do not favor the hypothesis that foreign mitochondrial DNA was pulled into the T. macedonicus range by natural selection.

  12. The structure of the shallow crust beneath Olkaria geothermal field, Kenya, deduced from gravity studies

    NASA Astrophysics Data System (ADS)

    Ndombi, J. M.

    1981-02-01

    The Olkaria region of the Kenya rift valley is a potentially important geothermal area due to the occurrence of young volcanic activity and surface geothermal manifestations which include hot springs, fumaroles and thermally altered ground. In order to determine subsurface structure nearly 255 new gravity measurements, together with existing regional gravity data, were used to propose a subsurface model. A few surface density measurements have been made within Olkaria and in adjoining areas, and these combined with density values inferred from the measured P-wave seismic velocities within Olkaria are used to define the density distribution within the shallow crust. A two-dimensional model to explain the local gravity anomalies indicates that an approximately horizontal three-layer volcanic sequence overlying the basement system is downfaulted on the western part of Olkaria, and is intruded by denser dyke-like bodies of rhyolitic composition along the north-south-trending fractures that occur mainly in the central part of the Olkaria. An increase in the thickness of volcanics in the western part of Olkaria is responsible for the observed negative Bouguer anomaly in that area, while vertical density contrasts between the dyke-like intrusions and the host rock generate model anomalies in good agreement with the observed positive Bouguer anomalies in the central part of Olkaria. The largest and most recent intrusion, which is probably still in a magmatic state, occurs along the Ololbutot fracture zone and is apparently the main heat source for the geothermal phenomena in Olkaria.

  13. Students' Dilemmas in Reaction Stoichiometry Problem Solving: Deducing the Limiting Reagent in Chemical Reactions

    ERIC Educational Resources Information Center

    Chandrasegaran, A. L.; Treagust, David F.; Waldrip, Bruce G.; Chandrasegaran, Antonia

    2009-01-01

    A qualitative case study was conducted to investigate the understanding of the limiting reagent concept and the strategies used by five Year 11 students when solving four reaction stoichiometry problems. Students' written problem-solving strategies were studied using the think-aloud protocol during problem-solving, and retrospective verbalisations…

  14. Structural and temporal requirements for geomagnetic field reversal deduced from lava flows.

    PubMed

    Singer, Brad S; Hoffman, Kenneth A; Coe, Robert S; Brown, Laurie L; Jicha, Brian R; Pringle, Malcolm S; Chauvin, Annick

    2005-03-31

    Reversals of the Earth's magnetic field reflect changes in the geodynamo--flow within the outer core--that generates the field. Constraining core processes or mantle properties that induce or modulate reversals requires knowing the timing and morphology of field changes that precede and accompany these reversals. But the short duration of transitional field states and fragmentary nature of even the best palaeomagnetic records make it difficult to provide a timeline for the reversal process. 40Ar/39Ar dating of lavas on Tahiti, long thought to record the primary part of the most recent 'Matuyama-Brunhes' reversal, gives an age of 795 +/- 7 kyr, indistinguishable from that of lavas in Chile and La Palma that record a transition in the Earth's magnetic field, but older than the accepted age for the reversal. Only the 'transitional' lavas on Maui and one from La Palma (dated at 776 +/- 2 kyr), agree with the astronomical age for the reversal. Here we propose that the older lavas record the onset of a geodynamo process, which only on occasion would result in polarity change. This initial instability, associated with the first of two decreases in field intensity, began approximately 18 kyr before the actual polarity switch. These data support the claim that complete reversals require a significant period for magnetic flux to escape from the solid inner core and sufficiently weaken its stabilizing effect.

  15. Comparative Analysis of Chloroplast Genomes: Functional Annotation, Genome-Based Phylogeny, and Deduced Evolutionary Patterns

    PubMed Central

    Rivas, Javier De Las; Lozano, Juan Jose; Ortiz, Angel R.

    2002-01-01

    All protein sequences from 19 complete chloroplast genomes (cpDNA) have been studied using a new computational method able to analyze functional correlations among series of protein sequences contained in complete proteomes. First, all open reading frames (ORFs) from the cpDNAs, comprising a total of 2266 protein sequences, were compared against the 3168 proteins from Synechocystis PCC6803 complete genome to find functionally related orthologous proteins. Additionally, all cpDNA genomes were pairwise compared to find orthologous groups not present in cyanobacteria. Annotations in the cluster of othologous proteins database and CyanoBase were used as reference for the functional assignments. Following this protocol, new functional assignments were made for ORFs of unknown function and for ycfs (hypothetical chloroplast frames), which still lack a functional assignment. Using this information, a matrix of functional relationships was derived from profiles of the presence and/or absence of orthologous proteins; the matrix included 1837 proteins in 277 orthologous clusters. A factor analysis study of this matrix, followed by cluster analysis, allowed us to obtain accurate phylogenetic reconstructions and the detection of genes probably involved in speciation as phylogenetic correlates. Finally, by grouping common evolutionary patterns, we show that it is possible to determine functionally linked protein networks. This has allowed us to suggest putative associations for some unknown ORFs. PMID:11932241

  16. Irradiation History of Itokawa Regolith Material Deduced from Noble Gases in the Hayabusa Samples

    NASA Astrophysics Data System (ADS)

    Nagao, Keisuke; Okazaki, Ryuji; Nakamura, Tomoki; Miura, Yayoi N.; Osawa, Takahito; Bajo, Ken-ichi; Matsuda, Shintaro; Ebihara, Mitsuru; Ireland, Trevor R.; Kitajima, Fumio; Naraoka, Hiroshi; Noguchi, Takaaki; Tsuchiyama, Akira; Yurimoto, Hisayoshi; Zolensky, Michael E.; Uesugi, Masayuki; Shirai, Kei; Abe, Masanao; Yada, Toru; Ishibashi, Yukihiro; Fujimura, Akio; Mukai, Toshifumi; Ueno, Munetaka; Okada, Tatsuaki; Yoshikawa, Makoto; Kawaguchi, Junichiro

    2011-08-01

    Noble gas isotopes were measured in three rocky grains from asteroid Itokawa to elucidate a history of irradiation from cosmic rays and solar wind on its surface. Large amounts of solar helium (He), neon (Ne), and argon (Ar) trapped in various depths in the grains were observed, which can be explained by multiple implantations of solar wind particles into the grains, combined with preferential He loss caused by frictional wear of space-weathered rims on the grains. Short residence time of less than 8 million years was implied for the grains by an estimate on cosmic-ray-produced 21Ne. Our results suggest that Itokawa is continuously losing its surface materials into space at a rate of tens of centimeters per million years. The lifetime of Itokawa should be much shorter than the age of our solar system.

  17. Identification of widespread pollution in the southern hemisphere deduced from satellite analyses. [AFRICA

    SciTech Connect

    Fishman, J. ); Fakhruzzaman, K. ); Cros, B.; Nganga, D. )

    1991-06-21

    Vertical profiles of ozone obtained from ozonesondes in Brazzaville, Congo (4{degree}S, 15{degree}E), and Ascension Island (98{degree}S, 15{degree}W) show that large quantities of tropospheric ozone are present over southern Africa and the adjacent eastern tropical South Atlantic Ocean. The origin of this pollution is widespread biomass burning in Africa. These measurements support satellite-derived tropospheric ozone data that demonstrate that ozone originating from the region is transported throughout most of the Southern Hemisphere. Seasonally high levels of carbon monoxide and methane observed at middle- and high-latitude stations in Africa, Australia, and Antarctica likely reflect the effects of this distant biomass burning. These data suggest that even the most remote regions on this planet may be significantly more polluted than previously believed.

  18. DNA Sequence-Dependent Deformability Deduced from Protein-DNA Crystal Complexes

    NASA Astrophysics Data System (ADS)

    Olson, Wilma K.; Gorin, Andrey A.; Lu, Xiang-Jun; Hock, Lynette M.; Zhurkin, Victor B.

    1998-09-01

    The deformability of double helical DNA is critical for its packaging in the cell, recognition by other molecules, and transient opening during biochemically important processes. Here, a complete set of sequence-dependent empirical energy functions suitable for describing such behavior is extracted from the fluctuations and correlations of structural parameters in DNA-protein crystal complexes. These elastic functions provide useful stereochemical measures of the local base step movements operative in sequence-specific recognition and protein-induced deformations. In particular, the pyrimidine-purine dimers stand out as the most variable steps in the DNA-protein complexes, apparently acting as flexible ``hinges'' fitting the duplex to the protein surface. In addition to the angular parameters widely used to describe DNA deformations (i.e., the bend and twist angles), the translational parameters describing the displacements of base pairs along and across the helical axis are analyzed. The observed correlations of base pair bending and shearing motions are important for nonplanar folding of DNA in nucleosomes and other nucleoprotein complexes. The knowledge-based energies also offer realistic three-dimensional models for the study of long DNA polymers at the global level, incorporating structural features beyond the scope of conventional elastic rod treatments and adding a new dimension to literal analyses of genomic sequences.

  19. Early chemo-dynamical evolution of dwarf galaxies deduced from enrichment of r-process elements

    NASA Astrophysics Data System (ADS)

    Hirai, Yutaka; Ishimaru, Yuhri; Saitoh, Takayuki R.; Fujii, Michiko S.; Hidaka, Jun; Kajino, Toshitaka

    2017-04-01

    The abundance of elements synthesized by the rapid neutron-capture process (r-process elements) of extremely metal-poor (EMP) stars in the Local Group galaxies gives us clues to clarify the early evolutionary history of the Milky Way halo. The Local Group dwarf galaxies would have similarly evolved with building blocks of the Milky Way halo. However, how the chemo-dynamical evolution of the building blocks affects the abundance of r-process elements is not yet clear. In this paper, we perform a series of simulations using dwarf galaxy models with various dynamical times and total mass, which determine star formation histories. We find that galaxies with dynamical times longer than 100 Myr have star formation rates less than 10-3 M⊙ yr-1 and slowly enrich metals in their early phase. These galaxies can explain the observed large scatters of r-process abundance in EMP stars in the Milky Way halo regardless of their total mass. On the other hand, the first neutron star merger appears at a higher metallicity in galaxies with a dynamical time shorter than typical neutron star merger times. The scatters of r-process elements mainly come from the inhomogeneity of the metals in the interstellar medium whereas the scatters of α-elements are mostly due to the difference in the yield of each supernova. Our results demonstrate that the future observations of r-process elements in EMP stars will be able to constrain the early chemo-dynamical evolution of the Local Group galaxies.

  20. Wheat - Its growth and disease severity as deduced from ERTS-1

    NASA Technical Reports Server (NTRS)

    Kanemasu, E. T.; Niblett, C. L.; Manges, H.; Lenhert, D.; Newman, M. A.

    1974-01-01

    The spectral reflectance of a cropped surface changes as the plant develops. An indicator of crop growth is leaf area index (ratio of green leaf area to soil area). The leaf area index, disease severity, and yield were determined for several winter wheat fields in Kansas during the 1973 growing season. Multispectral scanner (MSS) data from Earth Resources Technology Satellite-1 (ERTS-1) showed a high correlation (r greater than or equal to 0.90) between crop growth and MSS4/MSS5, and crop growth and MSS5/MSS6. Wheat disease severity and yields were significantly correlated at the 5% level with MSS4/MSS6 and with MSS4/MSS7. Further investigation is required before ERTS imagery can be routinely used detecting and estimating disease severity and yield reduction.

  1. An inverse technique to deduce the elasticity of a large artery

    NASA Astrophysics Data System (ADS)

    Lagrée, P.-Y.

    2000-02-01

    Our purpose is to build an inverse method which best fits a model of artery flow and experimental measurements (we assume that we are able to measure the displacement of the artery as a function of time at three stations). Having no clinical data, we simulate these measurements with the numerical computations from a "boundary layer" code. First, we revisit the system of Ling and Atabek of boundary layer type for the transmission of a pressure pulse in the arterial system for the case of an elastic wall (but we solve it without any simplification in the upartial u/partial x term). Then, using a method analogous to the well known Von Kármán-Pohlhausen method from aeronautics but transposed here for a pulsatile flow, we build a system of three coupled non-linear partial differential equations depending only on time and axial co-ordinate. This system governs the dynamics of internal artery radius, centre velocity and a quantity related to the presence of viscous effects. These two methods give nearly the same numerical results. Second, we construct an inverse method: the aim is to find for the simple integral model, the physical parameters to put in the "boundary layer" code (simulating clinical data). This is done by varying in the integral model the viscosity and elasticity in order to fit best with the data. To achieve this in a rational way, we have to minimise a cost function, which involves the computation of the adjoint system of the integral method. The good set of parameters ({i.e.} viscosity, and two coefficients of a wall law) is effectively found again. It opens the perspective for application in real clinical cases of this new non-invasive method for evaluating the viscosity of the flow and elasticity of the wall.

  2. Total Vertical Offset for the Beichuan Fault (Longmen Shan, Sichuan, China) Deduced from Metamorphic Minerals

    NASA Astrophysics Data System (ADS)

    Airaghi, L.; de Sigoyer, J.; Vidal, O.; Lanari, P.; Tan, X. B.; Xu, X.; Guillot, S.

    2015-12-01

    The paradox of high topography but low convergence rates in the Longmen Shan mountain belt, at the eastern margin of Tibetan plateau (Sichuan, China) leaded to an underestimation of the seismic hazard prior to the Wenchuan earthquake Mw 7.9 (2008). The rupture affected the crustal Beichuan fault, with both thrusting and dextral slide slip components. This fault is responsible for the exhumation of the basement over Triassic sediments. Several paleoseismological studies have well constrained the Quaternary activity of this fault, and thermochronological data show a rapid exhumation starting from Oligocene. The total offset of the Beichuan fault remains unknown. Samples collected in the hanging wall of the Beichuan fault, yield stable white mica, chlorite, epidote and quartz. Chemical and thermobarometric analyses of metamorphic minerals yield metamorphic peak conditions at 300±50°C and 7-8 kbar. Such P,T conditions suggest burial to 18-20 km depth, and represent the maximum vertical offset of the Beichuan fault. Comparison of results from distant sampled sites along the Beichuan fault reveals the spatial continuity of this offset along the Beichuan fault. The attainment of peak pressure conditions will be dated using in-situ laser ablation 40Ar/39Ar dating of the metamorphic micas. This will help constrain the onset of Beichuan fault activation, which corresponds to the onset of thick skin deformation in the Longmen Shan. [CW1]I deleted all this stuff below because I thought it was too much information for a conference abstract - but you do have space to put it back.

  3. Eastern Asian Emissions of Anthropogenic Halocarbons Deduced from Aircraft Concentration Data

    NASA Technical Reports Server (NTRS)

    Palmer, Paul I.; Jacob, Daniel J.; Mickle, Loretta, J.; Blake, Donald R.; Sachse, Glen W.; Fuelberg, Henry E.; Kiley, Christopher M.

    2003-01-01

    The Montreal Protocol restricts production of ozone-depleting halocarbons worldwide. Enforcement of the protocol has relied mainly on annual government statistics of production and consumption of these compounds (bottom-up approach). We show here that aircraft observations of ha1ocarbon:CO enhancement ratios on regional to continental scales can be used to infer halocarbon emissions, providing independent verification of the bottom-up approach. We apply this topdown approach to aircraft observations of Asian outflow &om the TRACE-P mission over the western Pacific (March-April 2001) and derive emissions from eastern Asia (China, Japan, and Korea). We derive an eastern Asian carbon tetrachloride (CCl4) source of 21.5 Gg yr(sup -1), several-fold larger than previous estimates and amounting to -30% of the global budget for this gas. Our emission estimate for CFC-11 from eastern Asia is 50% higher than inventories derived from manufacturing records. Our emission estimates for methyl chloroform (CH3CC13) and CFC-12 are in agreement with existing inventories. For halon 1211 we find only a strong local source originating from the Shanghai area. Our emission estimates for the above gases result in a approximately equal to 40% increase in the ozone depletion potential (ODP) of Asian emissions relative to previous estimates, corresponding to a approximately equal to 10% global increase in ODP.

  4. The structure of the magnetosphere as deduced from magnetospherically reflected whistlers

    NASA Technical Reports Server (NTRS)

    Edgar, B. C.

    1972-01-01

    Very low frequency (VLF) electromagnetic wave phenomenon called the magnetospherically reflected (MR) whistler was investigated. VLF (0.3 to 12.5 kHz) data obtained from the Orbiting Geophysical Observatories 1 and 3 from October 1964 to December 1966 were used. MR whistlers are produced by the dispersive propagation of energy from atmospheric lightning through the magnetosphere to the satellite along ray paths which undergo one or more reflections due to the presence of ions. The gross features of MR whistler frequency-time spectrograms are explained in terms of propagation through a magnetosphere composed of thermal ions and electrons and having small density gradients across L-shells. Irregularities observed in MR spectra were interpreted in terms of propagation through field-aligned density structures. Trough and enhancement density structures were found to produce unique and easily recognizable signatures in MR spectra. Sharp cross-field density dropoff produces extra traces in MR spectrograms.

  5. Shallow cumulus processes deduced from subcloud flux measurements and parameterization schemes

    SciTech Connect

    Oetles, Z.

    1993-01-01

    The purposes of this study are to improve understanding of shallow cumulus in the planetary boundary layer (PBL), to quantitatively measure their subcloud root processes, and to link those measurements to estimates of cloud layer processes. Subcloud aircraft turbulence data for three days from the 1986 HAPEX project in southwest France is extensively analyzed. Various parameterizations of cloud layer processes are used to estimate cumulus layer effects by employing the subcloud fluxes as boundary conditions. Case 1 (5/21/86) had active cumuli; over the forest they were deep and large in diameter, but clouds over farmland were shallow and small in diameter. Case 2 (5/9/86) has also weak active cumulus with a smaller vertical depth and case 3 (6/13/86) had only forced cumuli. Four methods of decomposition of turbulence data have been employed: by land surface-cumulus regime, by proximity to clouds and clear areas, by scale, and by turbulence process types. In the presence strong active cumuli from case 1, the turbulent fluxes and energy are intensified, due to the cloud [open quotes]root[close quotes] effects. The strong active cumuli are initiated by warm-moist thermals. Since cumulus layer flux measurements were not made during the HAPEX experiment, different parameterization schemes have been used to estimate cloud layer fluxes. The schemes are: the simple cumulus flux scheme, the Betts-Miller adjustment scheme, an eddy diffusion scheme, and a new eddy diffusion scheme with counter-gradient term which is developed as a part of this study. For the strong active cumulus regime from case 1, the liquid water potential temperature and total water fluxes obtained from different parameterization schemes yield very similar results. Unlike the other parameterization schemes, the new eddy diffusion scheme with a counter-gradient term yields upward buoyancy fluxes which are in a good agreement with earlier studies.

  6. Neutrino properties deduced from the study of lepton number violating processes at low and high energies

    SciTech Connect

    Stoica, Sabin

    2012-11-20

    There is nowadays a significant progress in understanding the neutrino properties. The results of the neutrino oscillation experiments have convincingly showed that neutrinos have mass and oscillate, in contradiction with the Standard Model (SM) assumptions, and these are the first evidences of beyond SM physics. However, fundamental properties of the neutrinos like their absolute mass, their character (are they Dirac or Majorana particles?), their mass hierarchy, the number of neutrino flavors, etc., still remain unknown. In this context there is an increased interest in the study of the lepton number violating (LNV) processes, since they could complete our understanding on the neutrino properties. Since recently, the neutrinoless double beta decay was considered the only process able to distinguish between Dirac or Majorana neutrinos and to give a hint on the absolute mass of the electron neutrino. At present, the increased luminosity of the LHC experiments makes feasible the search of LNV processes at high energy as well. In this lecture I will make a brief review on our present knowledge of the neutrino properties, on the present status of the double-beta decay studies and on the first attempts to search LNV processes at LHC.

  7. Education et Developpement. Une Approche aux Interventions d'Education en Milieux Defavorises. MEQ, Mars 1975.

    ERIC Educational Resources Information Center

    Centrale de L'Enseignement du Quebec (Canada).

    This document is a commentary on a report by the Quebec Ministry of Education on the problem of educating the disadvantaged. One basic criticism of the report is that it is not enough to decry the failure of the schools and propose a strategy of compensatory education to solve the problem of disadvantagement. It is necessary to realize that the…

  8. The mechanism of the acclimation of Nannochloropsis oceanica to freshwater deduced from its transcriptome profiles

    NASA Astrophysics Data System (ADS)

    Guo, Li; Yang, Guanpin

    2015-10-01

    In this study, we compared the transcriptomes of Nannochloropsis oceanica cultured in f/2 medium prepared with sea-water and freshwater, respectively, aiming to understand the acclimation mechanism of this alga to freshwater. Differentially expressed genes were mainly assigned to the degradation of cell components, ion transportation, and ribosomal biogenesis. These findings indicate that the algal cells degrade its components (mainly amino acids and fatty acids) to yield excessive energy (ATP) to maintain cellular ion (mainly K+ and Ca2+) homeostasis, while the depletion of amino acids and ATP, and the reduction of ribosomes attenuate the protein translation and finally slow down the cell growth.

  9. Comprehensive conformational studies of five tripeptides and a deduced method for efficient determinations of peptide structures.

    PubMed

    Yu, Wenbo; Wu, Zhiqing; Chen, Huibin; Liu, Xu; MacKerell, Alexander D; Lin, Zijing

    2012-02-23

    Thorough searches on the potential energy surfaces of five tripeptides, GGG, GYG, GWG, TGG, and MGG, were performed by considering all possible combinations of the bond rotational degrees of freedom with a semiempirical and ab initio combined computational approach. Structural characteristics of the obtained stable tripeptide conformers were carefully analyzed. Conformers of the five tripeptides were found to be closely connected with conformers of their constituting dipeptides and amino acids. A method for finding all important tripeptide conformers by optimizing a small number of trial structures generated by suitable superposition of the parent amino acid and dipeptide conformers is thus proposed. Applying the method to another five tripeptides, YGG, FGG, WGG, GFA, and GGF, studied before shows that the new approach is both efficient and reliable by providing the most complete ensembles of tripeptide conformers. The method is further generalized for application to larger peptides by introducing the breeding and mutation concepts in a genetic algorithm way. The generalized method is verified to be capable of finding tetrapeptide conformers with secondary structures of strands, helices, and turns, which are highly populated in larger peptides. This show some promise for the proposed method to be applied for the structural determination of larger peptides. © 2012 American Chemical Society

  10. Comprehensive Conformational Studies of Five Tripeptides and a Deduced Method for Efficient Determinations of Peptide Structures

    PubMed Central

    Yu, Wenbo; Wu, Zhiqing; Chen, Huibin; Liu, Xu; MacKerell, Alexander D.

    2012-01-01

    Thorough searches on the potential energy surfaces of five tripeptides, GGG, GYG, GWG, TGG and MGG, were performed by considering all possible combinations of the bond rotational degrees of freedom with a semi-empirical and ab initio combined computational approach. Structural characteristics of the obtained stable tripeptide conformers were carefully analyzed. Conformers of the five tripeptides were found to be closely connected with conformers of their constituting dipeptides and amino acids. A method for finding all important tripeptide conformers by optimizing a small number of trial structures generated by suitable superposition of the parent amino acid and dipeptide conformers is thus proposed. Applying the method to another five tripeptides, YGG, FGG, WGG, GFA and GGF, studied before shows that the new approach is both efficient and reliable by providing the most complete ensembles of tripeptide conformers. The method is further generalized for application to larger peptides by introducing the breeding and mutation concepts in a genetic algorithm way. The generalized method is verified to be capable of finding tetrapeptide conformers with secondary structures of strands, helices and turns which are highly populated in larger peptides. This show some promise for the proposed method to be applied for the structural determination of larger peptides. PMID:22260814

  11. Analysis of satellite data to deduce stratospheric constituents and UV spectroscopic properties of the atmosphere

    NASA Technical Reports Server (NTRS)

    Mcpeters, Richard D.

    1990-01-01

    The objective is to better understand the stratosphere, its constituents, and its ultraviolet optical properties, through detailed analysis of data from the SBUV instrument on Nimbus 7 and comparison with data from other instruments, including the NOAA 9 SBUV 2, SAGE, SME, and SMM. One conclusion to be drawn from the Ozone Trends Panel report is that there are unresolved differences in the ozone profiles measured by different instruments. While the purpose of the work is more to understand the details of the UV radiation field in the stratosphere than it is to assess the accuracy of the SBUV ozone measurement itself, improved understanding of specific problems in the UV will lead to more accurate ozone retrievals. Areas of study include the effect of aerosols on the backscattered albedo, the shape of the ozone profile near the stratopause, the effect of possible polar mesospheric clouds, and the measureability of nitric oxide and sulfur dioxide.

  12. Properties of solar coronal active regions deduced from X-ray line spectra

    NASA Astrophysics Data System (ADS)

    McKenzie, D. L.

    1987-11-01

    Spectra from the SOLEX B RAP spectrometer have been used to analyze the temperature and density structure of over 100 nonflaring solar active regions. Density measurements that used the R ratio of O VII indicated that few regions have electron densities higher than ≡3×109cm-3. In a few cases, flare-productive regions had measured densities approximately twice this high. Temperature-sensitive line ratios in the helium-like ions O VII, Ne IX, and Mg XI were used to decude the general properties of the differential emission-measure function B(T) for nonflaring regions. B(T) falls off with increasing temperature above a peak temperature that is almost always lower than Tm(O VII) = 1.8×106K.

  13. Variations of anthropogenic CO2 in urban area deduced by radiocarbon concentration in modern tree rings.

    PubMed

    Rakowski, Andrzej Z; Nakamura, Toshio; Pazdur, Anna

    2008-10-01

    Radiocarbon concentration in the atmosphere is significantly lower in areas where man-made emissions of carbon dioxide occur. This phenomenon is known as Suess effect, and is caused by the contamination of clean air with non-radioactive carbon from fossil fuel combustion. The effect is more strongly observed in industrial and densely populated urban areas. Measurements of carbon isotope concentrations in a study area can be compared to those from areas of clear air in order to estimate the amount of carbon dioxide emission from fossil fuel combustion by using a simple mathematical model. This can be calculated using the simple mathematical model. The result of the mathematical model followed in this study suggests that the use of annual rings of trees to obtain the secular variations of 14C concentration of atmospheric CO2 can be useful and efficient for environmental monitoring and modeling of the carbon distribution in local scale.

  14. Light elements burning reaction rates at stellar temperatures as deduced by the Trojan Horse measurements

    SciTech Connect

    Lamia, L.; Spitaleri, C.; La Cognata, M.; Palmerini, S.; Sergi, M. L.; Puglia, S. M. R.

    2015-02-24

    Experimental nuclear astrophysics aims at determining the reaction rates for astrophysically relevant reactions at their Gamow energies. For charged-particle induced reactions, the access to these energies is usually hindered, in direct measurements, by the presence of the Coulomb barrier between the interacting particles or by electron screening effects, which make hard the determination of the bare-nucleus S(E)-factor of interest for astrophysical codes. The use of the Trojan Horse Method (THM) appears as one of the most suitable tools for investigating nuclear processes of interest for astrophysics. Here, in view of the recent TH measurements, the main destruction channels for deuterium ({sup 2}H), for the two lithium {sup 6,7}Li isotopes, for the {sup 9}Be and the one for the two boron {sup 10,11}B isotopes will be discussed.

  15. Temperature and density dependence of properties of nuclear matter deduced from heavy ion collisions

    SciTech Connect

    Shlomo, Shalom

    2010-11-24

    Heavy-ion collision experiments are often employed to determine properties of nuclear matter under extreme conditions of temperature and density. This has been the subject of many investigations in recent decades, since understanding the equation of state of hot nuclear matter is very important in the study supernovae, neutron stars and nuclei. We present a short and limited review of the theoretical and experimental status of determining the temperature and density of the disassembling hot nucleus from ratios of the yields of emitted fragments.

  16. Crustal structure deduced from receiver functions via single-scattering migration

    NASA Astrophysics Data System (ADS)

    Bertrand, E.; Deschamps, A.; Virieux, J.

    2002-08-01

    An investigation of the teleseismic P-wave coda is performed using the single-scattering approximation. The method allows one to image short-wavelength scale (<=2 km) velocity and density heterogeneities and structures that are barely detected by traveltime tomography. Source effects are removed by using receiver functions for data interpretation, but the amplitude (especially the sign of the signal) is synthesized. Both broad-band seismological stations in the southwestern Alps (France) and Campanian plain (Italy) are used for the illustration of the proposed method. Because of the large aperture of our arrays, laterally small-scale heterogeneities are difficult to image and we must assume lateral continuity of the detectable structure. If so, we show that this depth migration based on a single-scattering approach can recover both the depth and the geometry of the main discontinuities below the studied areas. In the southwestern Alps, we underline a complex crustal structure and Moho dipping topography. The Moho depth increases from 20 to 30 km between the coastline and the Mercantour range. In the Campanian plain (surrounding Mount Vesuvius) we also find a southeastwards-dipping Moho. Furthermore, the three main discontinuities are imaged, showing a relatively shallow mantle-crust discontinuity and a deeper one near the coastline.

  17. Vertical profiles of dust and ozone in the Martian atmosphere deduced from solar occultation measurements

    NASA Astrophysics Data System (ADS)

    Blamont, J. E.; Chassefiere, E.; Goutail, J. P.; Mege, B.; Nunes-Pinharanda, M.; Souchon, G.; Krasnopolsky, V. A.; Krysko, A. A.; Moroz, V. I.

    1991-02-01

    The vertical distribution of the ozone content and of the aerosols in the Martian atmosphere at the equinox and near the equator was studied with the aid of a biaxial pointing device, a microprocessor-controlled flat mirror of elliptical shape. An upper limit of 5 x 10 to the 7th mol/cu cm for ozone was obtained above an altitude of 30 km. For the aerosols, a semiquantitative distribution has been obtained between 10 and 50 km of altitude. The scale height is nearly equal to the atmospheric scale height in the 10-20 km region where mixing seems to predominate, and falls rapidly to a thickness of about 2 km at 30 km. In 10 percent of the occultations, a stratified haze has been detected between 40 and 50 km. The particle radius of cloud constituents is estimated and optical thickness per kilometer of these hazes at peak extinction are approximated. An eddy diffusion coefficient and a mixing ratio are estimated for clouds assumed to be at equilibrium.

  18. Requirements for a marine geoid compatible with geoid deducible from satellite altimetry

    NASA Technical Reports Server (NTRS)

    Fubara, D. M. J.; Mourad, A. G.

    1972-01-01

    Theory deficiencies, data, and potential computational procedures that make the physical determination of the ocean geoid with true scale, shape, and absolute orientation of an elusive target are outlined. Satellite altimetry potential, in combination with adequate ground support and sea truth to resolve accurate global marine geoid and other peripheral benefits associated with ocean physics, are stated. Results are given in tabular form.

  19. The Hengill geothermal area, Iceland: variation of temperature gradients deduced from the maximum depth of seismogenesis

    USGS Publications Warehouse

    Foulger, G.R.

    1995-01-01

    Given a uniform lithology and strain rate and a full seismic data set, the maximum depth of earthquakes may be viewed to a first order as an isotherm. These conditions are approached at the Hengill geothermal area, S. Iceland, a dominantly basaltic area. The temperature at which seismic failure ceases for the strain rates likely at the Hengill geothermal area is determined by analogy with oceanic crust, and is about 650 ?? 50??C. The topographies of the top and bottom of the seismogenic layer were mapped using 617 earthquakes. The thickness of the seismogenic layer is roughly constant and about 3 km. A shallow, aseismic, low-velocity volume within the spreading plate boundary that crosses the area occurs above the top of the seismogenic layer and is interpreted as an isolated body of partial melt. The base of the seismogenic layer has a maximum depth of about 6.5 km beneath the spreading axis and deepens to about 7 km beneath a transform zone in the south of the area. -from Author

  20. The effect of subionospheric propagation on whistlers as deduced from direction finding measurements

    SciTech Connect

    Ohta, Kenji; Shimizu, Akio ); Hayakawa, Massashi )

    1994-01-15

    The authors report measurements made in South China of the propagation of intense whistler modes through the subionosphere. This provides a way to study the coupling between such ionospheric waves and the Earth-ionosphere waveguide modes. Strong circular polarization features were observed at frequencies near low order cutoff frequency modes. Measurement techniques include digital spectral analysis and field-analysis direction finding measurements.

  1. How historical seismicity in oceans can be deduced from sailors' testimonies and related to modern tectonics

    NASA Astrophysics Data System (ADS)

    Legrand, Denis; Rouland, Daniel; Cisternas, Armando; Streng, Daniel; Gir, Roopa; Souriau, Annie

    2016-04-01

    Before seismological catalogs were routinely produced, seafarers experienced major seismic events at sea that were documented in their logs. This article analyzes some of these old records - mostly from eighteenth and nineteenth centuries - in the context of plate tectonics. Large shocks that were felt on ships are related either to earthquakes, sub-marine volcanic eruptions, or to sub-marine sliding of rocks and/or sediments. We analyze various related parameters such as the location and size of the shaking, the duration of the shock, and the associated noise. A total of 396 observations have been retained for this study, mostly located in the Atlantic Ocean, reflecting its intense maritime traffic during the period of interest. Some of the detailed accounts allow us to clearly identify the nature of the shocks, including a possible interpretation in terms of focal mechanism. Our results, when compared to historical catalogs, reveal many previously undetected large events. Macroseismic results for a few large historical events occurring near the coasts confirm the validity of our approach, but also reveal its limitations. The good locations of most of the events allow us to relate them to plate boundaries. The Romanche transform zone has deserved particular interest due to the large number of related testimonies. This study particularly illustrates that historical seismicity may be applied to oceans. The collected testimonies also show how impressive and dangerous these large earthquakes at sea are, despite the absence of S-waves.

  2. Firing variability is higher than deduced from the empirical coefficient of variation.

    PubMed

    Ditlevsen, Susanne; Lansky, Petr

    2011-08-01

    A convenient and often used summary measure to quantify the firing variability in neurons is the coefficient of variation (CV), defined as the standard deviation divided by the mean. It is therefore important to find an estimator that gives reliable results from experimental data, that is, the estimator should be unbiased and have low estimation variance. When the CV is evaluated in the standard way (empirical standard deviation of interspike intervals divided by their average), then the estimator is biased, underestimating the true CV, especially if the distribution of the interspike intervals is positively skewed. Moreover, the estimator has a large variance for commonly used distributions. The aim of this letter is to quantify the bias and propose alternative estimation methods. If the distribution is assumed known or can be determined from data, parametric estimators are proposed, which not only remove the bias but also decrease the estimation errors. If no distribution is assumed and the data are very positively skewed, we propose to correct the standard estimator. When defining the corrected estimator, we simply use that it is more stable to work on the log scale for positively skewed distributions. The estimators are evaluated through simulations and applied to experimental data from olfactory receptor neurons in rats.

  3. Chemically-Deduced Star Formation Histories Of Dwarf Galaxies Using Barium

    NASA Astrophysics Data System (ADS)

    Duggan, Gina; Kirby, Evan

    2017-06-01

    Dwarf galaxies offer a unique opportunity to study the competing forces of galaxy evolution. Their simpler history (i.e., small size, fewer major mergers, and lack of active galactic nuclei) enables us to isolate different physical mechanisms more easily. The effects of these mechanisms are imprinted on the galaxy's star formation history. Traditionally, star formation histories are determined from color-magnitude diagrams. However, chemical abundances can increase the precision of this measurement. Here we present a simplistic galactic chemical evolution model to infer the star formation history. Chemical abundances are measured from spectra obtained with Keck/DEIMOS medium-resolution spectroscopy for over a hundred red giant stars from several satellite dwarf spheroidal galaxies and globular clusters. We focus our work on iron and barium abundances because they predominantly trace Type Ia supernovae and asymptotic giant branch stars, respectively. The different timescales of these two nucleosynthetic sources can be used to measure a finely resolved star formation history, especially when combined with existing [α/Fe] measurements. These models will inform the details of early star formation in dwarf galaxies and how it is affected by various physical processes, such as reionization and tidal stripping.

  4. Surface currents in the Caribbean Sea as deduced from Lagrangian observations

    NASA Astrophysics Data System (ADS)

    Molinari, Robert L.; Spillane, Michael; Brooks, Irving; Atwood, Donald; Duckett, Carol

    1981-07-01

    The results of a satellite tracked drifting buoy experiment conducted in the Caribbean Sea from October 1975 through June 1976 are presented. The buoy trajectories suggest that the Aves Rise, Beata Ridge, and Nicaraguan Rise are regions where considerable mesoscale variability occurs in the surface flow. The variability is in the form of current deflections; small diameter eddies ranging in size from 20 to 100 km; large amplitude meanders, with amplitudes greater than 150 km; and large diameter eddies, with diameters greater than 200 km which form on and near these rises and ridges. In the central Caribbean, where the topographic relief is not as dramatic, the flow is predominantly zonal. Intensifications in the surface flow occur off the coast of Colombia, south of the Nicaraguan Rise, and south of the Yucatan Strait.

  5. Longterm slip rate of the Pengguan Fault, deduced from river terraces along the Jinghe River

    NASA Astrophysics Data System (ADS)

    Xu, X.; Chen, Y.; Chung, L.; Chen, G.; Yu, G.; Tan, X.; Sun, X.; Jaiswal, M.; Shyu, J. H.; Lai, K.

    2009-12-01

    Two lines of surface ruptures, following the previously mapped Pengguan and Beichuan Fault, were produced during 2008 Wenchuan earthquake in Sichuan Province of China. The faults mentioned above actually are two frontal thrusts of the Longmenshan fold-and-thrust belt, which constitutes the eastern Tibetan plateau but lacks of the studies on slip rate except for the GPS observation. However, the time period of GPS observation is too short to entirely understand the fault kinematics. This study conducts the aerial photo mapping on river terraces and dating methods on terrace deposits to estimate the longterm slip rate and kinematically simulate the fault behaviors. For such a study river terraces fully distributed along the river which flows across the hanging wall of the thrust fault is usually required. Fortunately, along the valley of the Jinghe River, located in due west of Deyang, well-developed terraces are found in the hanging wall of the Pengguan fault. We used RTK GPS to reconstruct the elevation profile of the terraces. For the age determination on fluvial sediments, OSL, TL, and Radiocarbon methods are applied. Based on the longitudinal valley profile with the plot of the terrace heights, the terraces can be divided into 5 levels. By field observation it has been confirmed that the lower three levels are non-lateritic while the higher two are lateritic. Two lower terraces are also found already disrupted by a 3.2 m high fault scarp across the surface rupture of 2008 Wenchuan earthquake which produced only about 1.5 m vertical offset on the top of modern flood plain. Other higher terraces are distributed upstream, which are supposed to record the longterm deformation history of the Pengguan Fault. The geomorphic evidence presented in this study indicates the Pengguan Fault is a thrust dominant fault and at least 2 events have been recorded in the river terraces in ages of a few of thousand years. Accordingly, we use the terrace ages and morphology to derive the slip rate of the Pengguan Fault by a fault model that is suggested by the available geological information.

  6. Mass flux in the ecliptic plane and near the Sun deduced from Doppler scintillation

    NASA Technical Reports Server (NTRS)

    Woo, Richard; Gazis, Paul R.

    1994-01-01

    During the late declining phase of the solar cycle, the tilt of the solar magnetic dipole with respect to the Sun's rotation axis leads to large-scale organization of the solar wind, such that alternating regions of high- and low-speed solar wind are observed in the ecliptic plane. In this paper, we use Doppler scintillation measurements to investigate mass flux of these two types of solar wind in the ecliptic plane and inside 0.3 AU, where in situ measurements have not been possible. To the extent that Doppler scintillation reflects mass flux, we find that mass flux in high-speed streams: (1) is lower (by a factor of approximately 2.2) than the mass flux of the average solar wind in the heliocentric distance range of 0.3-0.5 AU; (2) is lower still (by as much as a factor of about 4) than the mass flux of the slow solar wind associated with the streamer belt; and (3) appears to grow with heliocentric distance. These Doppler scintillation results are consistent with the equator to pole decrease in mass flux observed in earlier spectral broadening measurements, and with trends and differences between high- and low-speed solar wind observed by in situ measurements in the range of 0.3-0.1 AU. The mass flux results suggest that the solar wind flow in high-speed streams is convergent towards the ecliptic near the Sun, becoming less convergent and approaching radial with increasing heliocentric distance beyond 0.3 AU. The variability of mass flux observed within equatorial and polar high-speed streams close to the Sun is strikingly low. This low variability implies that, as Ulysses currently ascends to higher latitudes and spends more time in the south polar high-speed stream after crossing the heliocentric current sheet, it can expect to observe a marked decrease in variations of both mass flux and solar wind speed, a trend that appears to have started already.

  7. Substrate binding mode and reaction mechanism of undecaprenyl pyrophosphate synthase deduced from crystallographic studies

    PubMed Central

    Chang, Sing-Yang; Ko, Tzu-Ping; Chen, Annie P.-C.; Wang, Andrew H.-J.; Liang, Po-Huang

    2004-01-01

    Undecaprenyl pyrophosphate synthase (UPPs) catalyzes eight consecutive condensation reactions of farnesyl pyrophosphate (FPP) with isopentenyl pyrophosphate (IPP) to form a 55-carbon long-chain product. We previously reported the crystal structure of the apo-enzyme from Escherichia coli and the structure of UPPs in complex with sulfate ions (resembling pyrophosphate of substrate), Mg2+, and two Triton molecules (product-like). In the present study, FPP substrate was soaked into the UPPs crystals, and the complex structure was solved. Based on the crystal structure, the pyrophosphate head group of FPP is bound to the backbone NHs of Gly29 and Arg30 as well as the side chains of Asn28, Arg30, and Arg39 through hydrogen bonds. His43 is close to the C2 carbon of FPP and may stabilize the farnesyl cation intermediate during catalysis. The hydrocarbon moiety of FPP is bound with hydrophobic amino acids including Leu85, Leu88, and Phe89, located on the α3 helix. The binding mode of FPP in cis-type UPPs is apparently different from that of trans-type and many other prenyltransferases which utilize Asprich motifs for substrate binding via Mg2+. The new structure provides a plausible mechanism for the catalysis of UPPs. PMID:15044730

  8. An interplanetary magnetic field enhancement observed by five spacecraft: Deducing the magnetic structure, size and mass

    NASA Astrophysics Data System (ADS)

    Lai, H.; Russell, C. T.; Delzanno, G.; Angelopoulos, V.

    2012-12-01

    Interplanetary Field Enhancements (IFEs) were discovered almost 30 years ago in the PVO magnetic-field records and attributed to the interaction between solar wind and dust particles from comets or asteroids, but the physics of this interaction remained obscure. Our current understanding is that IFEs result from collisions of small interplanetary bodies that produce electrically charged nanometer-scale dust particles possibly enhanced by tribo-electric charging in the collision. These charged dust particles in turn interact with the magnetized solar wind. Momentum is transferred from the solar wind to the dust cloud via the collective effect of the formation of a magnetic barrier. This momentum transfer accelerates the particles to near the solar wind speed and moves the dust outward through the solar gravitational potential well. Multi-spacecraft observations can help us to determine the speed of the IFE and the orientation of the current sheet. They enable us to reconstruct the pressure profile of an IFE in three dimensions and estimate the mass contained in the IFE. We have done these reconstructions with an IFE observed on March 3, 2011 with Wind, ACE, ARTEMIS P1 and P2 and Geotail. We find that the magnetic field near the center of the IFE is highly twisted indicating a complicated magnetic topology as expected in a plasma-charged dust interaction. The magnetic field and plasma properties during this event distinguish it from a typical flux rope. Based on the statistical results obtained at 1 AU and the assumption that all the IFEs are self-similar, we find that this IFE has a radial scale length several times longer than the cross flow radius and contains a mass of about 108 kg. The rates of collisions expected for objects of this size are consistent with the observed rates of these disturbances.

  9. Proxy late Holocene climatic record deduced from northwest Alaska beach ridges

    SciTech Connect

    Mason, O.K.; Jordan, J.W.

    1992-03-01

    A climatically sensitive, oscillatory pattern of progradation and erosion is revealed in late Holocene accretionary sand ridge and barrier island complexes of Seward Peninsula, northwest Alaska. Archaeological and geological radiocarbon dates constrain the chronology for the Cape Espenberg beach ridge plain and the Shishmaref barrier islands, 50 km to the southwest. Cape Espenberg, the depositional sink for the northeastward longshore transport system, contains the oldest sedimentary deposits: 3700 +/- 90 B.P. (B-23170) old grass from a paleosol in a low dune. The oldest date on the Shishmaref barrier islands is 1550 +/- 70 B.P. (B-23183) and implies that the modern barrier is a comparatively recent phenomenon. Late Holocene sedimentation along the Seward Peninsula varied between intervals of rapid progradation and erosion. Rapid progradation predominated from 4000-3300 B.P. and from 2000-1200 B.P., with the generation of low beach ridges without dunes, separated by wide swales. During erosional periods higher dunes built atop beach ridges: as between 3300-2000 B.P. and intermittently from 1000 B.P. to the present. Dune formation correlates with the Neoglacial and Little Ice Age glacial advances and increased alluviation in northern and central Alaska, while rapid progradation is contemporaneous with warmer intervals of soil and/or, peat formation atop alluvial terraces, dated to 4000-3500 and 2000-1000 B.P.

  10. Evolution of geothermal fluids deduced from chemistry plots: Yellowstone National Park (U.S.A.)

    NASA Astrophysics Data System (ADS)

    Mazor, Emanuel; Thompson, J. M.

    1982-05-01

    Large amounts of chemical data, obtained in geothermal fields, may readily be sorted-out by the aid of a simple set of graphs that provide a clear over-all picture and facilitate the understanding of geochemical processes taking place. As a case study, data from several hundred samples of the thermal springs at the well-known Yellowstone National Park are discussed. The pattern obtained seems to indicate: (1) geochemical similarity between the spring groups of Heart Lake, Shoshone, Upper, Midway, Lower and Norris Geyser Basins, i.e., a geochemical uniformity of major spring groups located over 40 km apart; (2) these groups may be described as originating from a common fluid, most resembling the composition of Norris waters, accompanied by CO 2, and other volatiles, that react with igneous rocks, forming local variations; (3) the secondary reactions occur at (medium) depth, before the ascent to the surface; (4) extensive concentration-dilution processes occur during the ascent to the surface. The water of the Mammoth group may be described as originating from the same Norris-like fluid that has been diluted (low Na and Cl contents) and intensively reacted with carbonaceous rocks, thus gaining in Ca, Mg, SO 4, and HCO 3.

  11. Low-latitude ionosphere dynamics as deduced from meridional ionosonde chain: Ionospheric ceiling

    NASA Astrophysics Data System (ADS)

    Maruyama, Takashi; Uemoto, Junpei; Tsugawa, Takuya; Supnithi, Pornchai; Ishii, Mamoru; Komolmis, Tharadol

    Interest in the equatorial anomaly in the ionosphere has been focused mostly on f_oF_2, and not much attention was paid to h_mF_2 except for the time rate of change of it in connection with the vertical plasma drift velocity. There have been few climatological studies on h_mF_2 variations associated with development of the equatorial anomaly. In this paper, we revisit the equatorial anomaly in terms of height variations. For this purpose, we analyzed scaled ionogram parameters from three stations located along the magnetic meridian that is a primary component of Southeast Asia low-latitude ionospheric network (SEALION); one at the magnetic equator and the others at conjugate off-equatorial latitudes near 10 degrees magnetic latitude. The daytime h_mF_2 was investigated for each season during the solar minimum period, 2006-2007 and 2009. The peak height increased for approximately 3 hr after sunrise at all locations, as expected from the daytime upward E×B drift. The apparent upward drift ceased before noon at the magnetic equator, while the layer continued to increase at the off-equatorial latitudes, reaching altitudes higher than the equatorial height around noon. The noon time restricted layer height at the magnetic equator did not depend much on the season, while the maximum peak height at the off-equatorial latitudes largely varied with season. The daytime specific limiting height of the equatorial ionosphere was termed ionospheric ceiling. Numerical modeling using the SAMI2 code reproduced the features of the ionospheric ceiling quite well. Dynamic parameters provided by the SAMI2 modeling were investigated and it was shown that the ionospheric ceiling is another aspect of the fountain effect, in which increased diffusion of plasma at higher altitudes has a leading role.

  12. Glycan family analysis for deducing N-glycan topology from single MS

    PubMed Central

    Goldberg, David; Bern, Marshall; North, Simon J.; Haslam, Stuart M.; Dell, Anne

    2009-01-01

    Motivation: In the past few years, mass spectrometry (MS) has emerged as the premier tool for identification and quantification of biological molecules such as peptides and glycans. There are two basic strategies: single-MS, which uses a single round of mass analysis, and MS/MS (or higher order MSn), which adds one or more additional rounds of mass analysis, interspersed with fragmentation steps. Single-MS offers higher throughput, broader mass coverage and more direct quantitation, but generally much weaker identification. Single-MS, however, does work fairly well for the case of N-glycan identification, which are more constrained than other biological polymers. We previously demonstrated single-MS identification of N-glycans to the level of ‘cartoons’ (monosaccharide composition and topology) by a system that incorporates an expert's detailed knowledge of the biological sample. In this article, we explore the possibility of ab initio single-MS N-glycan identification, with the goal of extending single-MS, or primarily-single-MS, identification to non-expert users, novel conditions and unstudied tissues. Results: We propose and test three cartoon-assignment algorithms that make inferences informed by biological knowledge about glycan synthesis. To test the algorithms, we used 71 single-MS spectra from a variety of tissues and organisms, containing more than 2800 manually annotated peaks. The most successful of the algorithms computes the most richly connected subgraph within a ‘cartoon graph’. This algorithm uniquely assigns the correct cartoon to more than half of the peaks in 41 out of the 71 spectra. Contact: goldberg@parc.com Supplementary information: Supplementary data are available at Bioinformatics online. PMID:19073587

  13. Seasonal and global cloud variations deduced from polar orbiting satellite radiance measurements

    NASA Technical Reports Server (NTRS)

    Rossow, W. B.; Kinsella, E.; Garder, L.

    1983-01-01

    The utility of currently available satellite radiance data for determining cloud radiative effects is evaluated. Each location observed by satellite is classified as clear or cloudy by comparison of measured radiances with specified values. Clear scene radiances are functions of time and location which represent the actual variation of the surface and atmospheric properties that affect the measured radiances. Cloud properties are determined by comparison of model and observed radiance. The seasonal change in cloud properties is shown, displaying the difference between July and January monthly mean values of cloud cover fraction, cloud top temperature, cloud top altitude, and cloud optical thickness.

  14. A 350 Year Cloud Cover Reconstruction Deduced from Caribbean Coral Proxies

    NASA Astrophysics Data System (ADS)

    Winter, Amos; Sammarco, Paul; Mikolajewicz, Uwe; Jury, Mark; Zanchettin, Davide

    2015-04-01

    Clouds are a major factor contributing to climate change with respect to a variety of effects on the earth's climates, primarily radiative effects, amelioration of heating, and regional changes in precipitation patterns. There have been very few studies of decadal and longer term changes in cloud cover in the tropics and sub-tropics, both over land and the ocean. In the tropics, there is great uncertainty regarding how global warming will affect cloud cover. Observational satellite data is so short that it is difficult to discern any temporal trends. The skeletons of scleractinian corals are considered to contain among the best records of high-resolution (sub-annual) environmental variability in the tropical and sub-tropical oceans. Corals generally live in well-mixed coastal regions and can often record environmental conditions of large areas of the upper ocean. This is particularly the case at low latitudes. Scleractinian corals are sessile, epibenthic fauna, and the type of environmental information recorded at the location where the coral has been living is dependent upon the species of coral considered and proxy index of interest. Zooxanthellate hermatypic corals in tropical and sub-tropical seas precipitate CaCO3 skeletons as they grow. This growth is made possible through the manufacture of CaCO3 crystals, facilitated by the zooxanthellae. During the process of crystallization, the holobiont binds carbon of different isotopes into the crystals. Stable carbon isotope concentrations vary with a variety of environmental conditions. In the Caribbean, δ13C in corals of the species Montastraea faveolata can be used as a proxy for changes in cloud cover. In this contribution, we will demonstrate that the stable isotope 13C varies concomitantly with cloud cover and present a new reconstruction of cloud cover over the Caribbean Sea that extends back to the year 1760. We will show that there is good agreement between the main features of our coral proxy record of cloud cover and of reanalysis and climate simulations for the same time period.

  15. Possible origin of a B chromosome deduced from its DNA composition using double FISH technique.

    PubMed

    López-León, M D; Neves, N; Schwarzacher, T; Heslop-Harrison, J S; Hewitt, G M; Camacho, J P

    1994-03-01

    Double fluorescent in situ hybridization (FISH) with two DNA probes (a 180 bp tandemly repeated DNA and ribosomal DNA) was performed in embryo cells of the grasshopper Eyprepocnemis plorans. Repetitive DNA was present in most standard chromosomes (excepting 7, 8 and 10) and in the proximal two-thirds of the B chromosome, which was its major location in the complement. Ribosomal DNA was present distally on the B, and in the active nucleolar organizer regions (NORs) of the X, 9, 10 and 11 chromosomes. A small number of rRNA gene clusters was also observed in the pericentromeric regions of chromosomes 1-8. The double FISH technique showed that the B chromosome (B2 type) is mainly composed of a 180 bp tandem repeat and ribosomal DNA, the minute short arm being the only region that does not hybridize with them. The location and order of the centromere and both the DNA sequences on the B chromosome coincide only with those in the X chromosome, indicating that the B most probably derives from the X.

  16. Paleoclimate in north-east Anatolia during the Quaternary deduced from glacial archives

    NASA Astrophysics Data System (ADS)

    Reber, R.; Akçar, N.; Yavuz, V.; Tikhomirov, D.; Vockenhuber, C.; Kubik, P. W.; Schlüchter, C.

    2012-04-01

    Today's climate of the northern part of the eastern Black Sea Mountains is humid with a mean annual precipitation over 2000 mm. The moisture is directly transported from the Black Sea. Field evidence indicate that this area was glaciated during past. Ice build-up can be therefore explained by a moisture transport system similar to today. Analogous to the north, the southern part of these mountains is relatively dry, although the field evidence in Çoruh Valley in Yusufeli, for instance, point to even more extensive glaciations. To accumulate more ice volume in the southern part, a different moisture transport than today is needed, for instance the moisture transport path may have changed in the past. But the time when this ice-build up (recorded by glacial deposits and erosional features) took place is still unclear. To construct the chronology of these glaciations with cosmogenic 10Be and 36Cl, we collected 14 samples from erratic boulders and glacially abraded bedrock in the Çoruh Valley in Yusufeli in the southern part and 41 samples in the Başyayla valley in the northern part. According to the existing data, glaciers advanced around 21 ka in the Kavron and Verçenik valleys in the northern part during the Last Glacial Maximum (LGM) (Akçar et al. 2007; 2008). While a Lateglacial advance archived around 15 ka in the Verçenik valley, Younger Dryas is recorded around 12 ka in the Kavron valley. Our first results from Çoruh valley indicate presence of two pre-LGM glaciations (>35 ka and >70 ka), a LGM advance (not dated yet) and a Lateglacial extent around 14 ka. Both field and dating evidence from the southern part of the eastern Black Sea Mountains enriches our knowledge about the glacier build-up and moisture transport during the Late Pleistocene, which can be interesting for our understanding of changes atmospheric circulation patterns during colder periods.

  17. Jupiter's atmospheric composition and cloud structure deduced from absorption bands in reflected sunlight

    NASA Technical Reports Server (NTRS)

    Sato, M.; Hansen, J. E.

    1979-01-01

    The spectrum of sunlight reflected by Jupiter is analyzed by comparing observations of Woodman (1979) with multiple-scattering computations. The analysis yields information on the vertical cloud structure at several latitudes and on the abundance of CH4 and NH3 in the atmosphere of Jupiter. The abundances of CH4 and NH3 suggest that all ices and rocks are overabundant on Jupiter by a factor of 2 or more, providing an important constraint on models for the formation of Jupiter from the primitive solar nebula. The pressure level of the clouds, the gaseous NH3 abundance, the mean temperature profile, and the Clausius-Clapeyron relation suggest that these clouds are predominantly ammonia crystals with the cloud bottom at 600-700 mb. A diffuse distribution of aerosols exists between 150 and 500 mb, and the spectral variation of albedo reflects a changing bulk absorption coefficient of the material composing the aerosols and is diagnostic of the aerosol composition.

  18. The Energy of Olivine Grain Boundaries Deduced from Grain Boundary Frequency Analyses

    NASA Astrophysics Data System (ADS)

    Marquardt, K.; Rohrer, G. S.

    2015-12-01

    The properties of grain boundaries strongly differ from those of the crystal lattice, and there is growing evidence that the presence of grain boundaries influence detected geophysical signals such as electrical conductivity and seismic velocities especially in aggregates with a LPO that favours the alignment of specific grain boundaries. However, neither the anisotropic frequency or energy distribution of grain boundary networks are understood in olivine dominated aggregates, neither with nor without LPO. We used electron backscatter diffraction, EBSD to detect the orientations of over 1.4x104 grains corresponding to roughly 5000mm length of grain boundary separating them. Subsequently we used a stereological approach to determine the grain boundary character distribution, GBCD, defined as the relative areas of grain boundaries of different types, distinguished by their five degrees of freedom (Rohrer, 2007). The grain boundary planes showed a preference for low index planes, which is in agreement with recent findings on other materials (e.g. MgO, TiO2, SrTiO3, MgAl2O4). However, our inferred surface energies are controversial with respect to previously simulated surface energies (Watson et al., 1997; de Leeuw et al., 2000; Gurmani et al., 2011). We find that the principal crystallographic planes have the lowest energies and that at 60° misorientation specific grain boundaries with common [100] axis of misorientation are favored compared to 60° misorientations about random axis of rotation. This seems to support the results of (Faul and Fitz Gerald, 1999), even though our data imply that 90°/[001] (100)(010) should be even less favorable for the propagation of melt films. These differences and similarities will be discussed with respect to the different methods and their limitations. References: Faul U. H. and Fitz Gerald J. D. (1999) Phys. Chem. Miner. 26, 187-197. Gurmani S. F. et al. (2011). J. Geophys. Res. 116, B12209. De Leeuw N. H. et al. (2000) Phys. Chem. Miner. 27, 332-341. Rohrer G. S. et al. (2004) Z. Metallkd. 95, 1-18. Watson G. W., Oliver P. M. and Parker S. C. (1997) Phys. Chem. Miner. 25, 70-78.

  19. Neptune’s global circulation deduced from multi-wavelength observations

    NASA Astrophysics Data System (ADS)

    de Pater, Imke; Fletcher, Leigh N.; Luszcz-Cook, Statia; DeBoer, David; Butler, Bryan; Hammel, Heidi B.; Sitko, Michael L.; Orton, Glenn; Marcus, Philip S.

    2014-07-01

    We observed Neptune between June and October 2003 at near- and mid-infrared wavelengths with the 10-m W.M. Keck II and I telescopes, respectively; and at radio wavelengths with the Very Large Array. Images were obtained at near-infrared wavelengths with NIRC2 coupled to the adaptive optics system in both broad- and narrow-band filters between 1.2 and 2.2 μm. In the mid-infrared we imaged Neptune at wavelengths between 8 and 22 μm, and obtained slit-resolved spectra at 8-13 μm and 18-22 μm. At radio wavelengths we mapped the planet in discrete filters between 0.7 and 6 cm. We analyzed each dataset separately with a radiative-transfer program that is optimized for that particular wavelength regime. At southern midlatitudes the atmosphere appears to be cooler at mid-infrared wavelengths than anywhere else on the planet. We interpret this to be caused by adiabatic cooling due to air rising at midlatitudes at all longitudes from the upper troposphere up to ≲0.1 mbar levels. At near-infrared wavelengths we find two distinct cloud layers at these latitudes: a relatively deep layer of clouds (presumably methane) in the troposphere at pressure levels P∼300-≳600 mbar, which we suggest to be caused by the large-scale upwelling and its accompanying adiabatic cooling and condensation of methane; and a higher, spatially intermittent, layer of clouds in the stratosphere at 20-30 mbar. The latitudes of these high clouds encompass an anticyclonic band of zonal flow, which suggests that they may be due to strong, but localized, vertical upwellings associated with local anticyclones, rather than plumes in convective (i.e., cyclonic) storms. Clouds at northern midlatitudes are located at the highest altitudes in the atmosphere, near 10 mbar. Neptune’s south pole is considerably enhanced in brightness at both mid-infrared and radio wavelengths, i.e., from ∼0.1 mbar levels in the stratosphere down to tens of bars in the troposphere. We interpret this to be due to subsiding motions from the stratosphere all the way down to the deep troposphere. The enhanced brightness observed at mid-infrared wavelengths is interpreted to be due to adiabatic heating by compression in the stratosphere, and the enhanced brightness temperature at radio wavelengths reveals that the subsiding air over the pole is very dry; the relative humidity of H2S over the pole is only 5% at altitudes above the NH4SH cloud at ∼40 bar. The low humidity region extends from the south pole down to latitudes of 66°S. This is near the same latitudes as the south polar prograde jet signifying the boundary of the polar vortex. We suggest that the South Polar Features (SPFs) at latitudes of 60-70° are convective storms, produced by baroclinic instabilities expected to be produced at latitudes near the south polar prograde jet. Taken together, our data suggest a global circulation pattern where air is rising above southern and northern midlatitudes, from the troposphere up well into the stratosphere, and subsidence of dry air over the pole and equator from the stratosphere down into the troposphere. We suggest that this pattern extends all the way from ≲0.1 mbar down to pressures of ≳40 bar.

  20. Lower mantle thermal structure deduced from seismic tomography, mineral physics and numerical modelling

    NASA Technical Reports Server (NTRS)

    Cadek, O.; Yuen, D. A.; Steinbach, V.; Chopelas, A.; Matyska, C.

    1994-01-01

    The long-wavelength thermal anomalies in the lower mantle have been mapped out using several seismic tomographic models in conjunction with thermodynamic parameters derived from high-pressure mineral physics experiments. These parameters are the depth variations of thermal expansivity and of the proportionality factor between changes in density and seismic velocity. The giant plume-like structures in the lower mantle under the Pacific Ocean and Africa have outer fringes with thermal anomalies around 300-400 K, but very high temperatures are found in the center of the plumes near the base of the core-mantle boundary. These extreme values can exceed +1500 K and may reflect large hot thermal anomalies in the lower mantle, which are supported by recent measurements of high melting temperatures of perovskite and iron. Extremely cold anomalies, around -1500 K, are found for anomalies in the deep mantle around the Pacific rim and under South America. Numerical simulations show that large negative thermal anomalies in the mid-lower mantle have modest magnitudes of around -500 K. correlation pattern exists between the present-day locations of cold masses in the lower mantle and the sites of past subduction since the Cretaceous. Results from correlation analysis show that the slab mass-flux in the lower mantle did not conform to a steady-state nature but exhibited time-dependent behavior.

  1. Students' Dilemmas in Reaction Stoichiometry Problem Solving: Deducing the Limiting Reagent in Chemical Reactions

    ERIC Educational Resources Information Center

    Chandrasegaran, A. L.; Treagust, David F.; Waldrip, Bruce G.; Chandrasegaran, Antonia

    2009-01-01

    A qualitative case study was conducted to investigate the understanding of the limiting reagent concept and the strategies used by five Year 11 students when solving four reaction stoichiometry problems. Students' written problem-solving strategies were studied using the think-aloud protocol during problem-solving, and retrospective verbalisations…

  2. Constructing a folding model for protein S6 guided by native fluctuations deduced from NMR structures

    SciTech Connect

    Lammert, Heiko; Noel, Jeffrey K.; Haglund, Ellinor; Onuchic, José N.; Schug, Alexander

    2015-12-28

    The diversity in a set of protein nuclear magnetic resonance (NMR) structures provides an estimate of native state fluctuations that can be used to refine and enrich structure-based protein models (SBMs). Dynamics are an essential part of a protein’s functional native state. The dynamics in the native state are controlled by the same funneled energy landscape that guides the entire folding process. SBMs apply the principle of minimal frustration, drawn from energy landscape theory, to construct a funneled folding landscape for a given protein using only information from the native structure. On an energy landscape smoothed by evolution towards minimal frustration, geometrical constraints, imposed by the native structure, control the folding mechanism and shape the native dynamics revealed by the model. Native-state fluctuations can alternatively be estimated directly from the diversity in the set of NMR structures for a protein. Based on this information, we identify a highly flexible loop in the ribosomal protein S6 and modify the contact map in a SBM to accommodate the inferred dynamics. By taking into account the probable native state dynamics, the experimental transition state is recovered in the model, and the correct order of folding events is restored. Our study highlights how the shared energy landscape connects folding and function by showing that a better description of the native basin improves the prediction of the folding mechanism.

  3. Stratospheric warmings in the Southern Hemisphere deduced from satellite radiation data, 1969-73

    NASA Technical Reports Server (NTRS)

    Quiroz, R. S.

    1974-01-01

    The investigation is largely based on radiation data from the vertical temperature profile radiometers on the satellite NOAA 2. The physical-statistical interpretation of the radiance data is considered. Aspects regarding the nonuniqueness of the minor warming in August 1973 are discussed. It is pointed out that conditions leading to major warmings in the Northern Hemisphere were not satisfied in the Southern Hemisphere during the period from 1969 to 1973.

  4. Lower mantle thermal structure deduced from seismic tomography, mineral physics and numerical modelling

    NASA Technical Reports Server (NTRS)

    Cadek, O.; Yuen, D. A.; Steinbach, V.; Chopelas, A.; Matyska, C.

    1994-01-01

    The long-wavelength thermal anomalies in the lower mantle have been mapped out using several seismic tomographic models in conjunction with thermodynamic parameters derived from high-pressure mineral physics experiments. These parameters are the depth variations of thermal expansivity and of the proportionality factor between changes in density and seismic velocity. The giant plume-like structures in the lower mantle under the Pacific Ocean and Africa have outer fringes with thermal anomalies around 300-400 K, but very high temperatures are found in the center of the plumes near the base of the core-mantle boundary. These extreme values can exceed +1500 K and may reflect large hot thermal anomalies in the lower mantle, which are supported by recent measurements of high melting temperatures of perovskite and iron. Extremely cold anomalies, around -1500 K, are found for anomalies in the deep mantle around the Pacific rim and under South America. Numerical simulations show that large negative thermal anomalies in the mid-lower mantle have modest magnitudes of around -500 K. correlation pattern exists between the present-day locations of cold masses in the lower mantle and the sites of past subduction since the Cretaceous. Results from correlation analysis show that the slab mass-flux in the lower mantle did not conform to a steady-state nature but exhibited time-dependent behavior.

  5. Deduced amino acid sequence of human pulmonary surfactant proteolipid: SPL(pVal)

    SciTech Connect

    Whitsett, J.A.; Glasser, S.W.; Korfhagen, T.R.; Weaver, T.E.; Clark, J.; Pilot-Matias, T.; Meuth, J.; Fox, J.L.

    1987-05-01

    Hydrophobic, proteolipid-like protein of Mr 6500 was isolated from ether/ethanol extracts of human, canine and bovine pulmonary surfactant. Amino acid composition of the protein demonstrated a remarkable abundance of hydrophobic residues, particularly valine and leucine. The N-terminal amino acid sequence of the human protein was determined: N-Leu-Ile-Pro-Cys-Cys-Pro-Val-Asn-Leu-Lys-Arg-Leu-Leu-Ile-Val4... An oligonucleotide probe was used to screen an adult human lung cDNA library and resulted in detection of cDNA clones with predicted amino acid sequence with close identity to the N-terminal amino acid sequence of the human peptide. SPL(pVal) was found within the reading frame of a larger peptide. SPL(pVal) results from proteolytic processing of a larger preprotein. Northern blot analysis detected in a single 1.0 kilobase SPL(pVal) RNA which was less abundant in fetal than in adult lung. Mixtures of purified canine and bovine SPL(pVal) and synthetic phospholipids display properties of rapid adsorption and surface tension lowering activity characteristic of surfactant. Human SPL(pVal) is a pulmonary surfactant proteolipid which may therefore be useful in combination with phospholipids and/or other surfactant proteins for the treatment of surfactant deficiency such as hyaline membrane disease in newborn infants.

  6. Evolution of green plants as deduced from 5S rRNA sequences.

    PubMed

    Hori, H; Lim, B L; Osawa, S

    1985-02-01

    We have constructed a phylogenic tree for green plants by comparing 5S rRNA sequences. The tree suggests that the emergence of most of the uni- and multicellular green algae such as Chlamydomonas, Spirogyra, Ulva, and Chlorella occurred in the early stage of green plant evolution. The branching point of Nitella is a little earlier than that of land plants and much later than that of the above green algae, supporting the view that Nitella-like green algae may be the direct precursor to land plants. The Bryophyta and the Pteridophyta separated from each other after emergence of the Spermatophyta. The result is consistent with the view that the Bryophyta evolved from ferns by degeneration. In the Pteridophyta, Psilotum (whisk fern) separated first, and a little later Lycopodium (club moss) separated from the ancestor common to Equisetum (horsetail) and Dryopteris (fern). This order is in accordance with the classical view. During the Spermatophyta evolution, the gymnosperms (Cycas, Ginkgo, and Metasequoia have been studied here) and the angiosperms (flowering plants) separated, and this was followed by the separation of Metasequoia and Cycas (cycad)/Ginkgo (maidenhair tree) on one branch and various flowering plants on the other.

  7. Evolution of green plants as deduced from 5S rRNA sequences

    PubMed Central

    Hori, Hiroshi; Lim, Byung-Lak; Osawa, Syozo

    1985-01-01

    We have constructed a phylogenic tree for green plants by comparing 5S rRNA sequences. The tree suggests that the emergence of most of the uni- and multicellular green algae such as Chlamydomonas, Spirogyra, Ulva, and Chlorella occurred in the early stage of green plant evolution. The branching point of Nitella is a little earlier than that of land plants and much later than that of the above green algae, supporting the view that Nitella-like green algae may be the direct precursor to land plants. The Bryophyta and the Pteridophyta separated from each other after emergence of the Spermatophyta. The result is consistent with the view that the Bryophyta evolved from ferns by degeneration. In the Pteridophyta, Psilotum (whisk fern) separated first, and a little later Lycopodium (club moss) separated from the ancestor common to Equisetum (horsetail) and Dryopteris (fern). This order is in accordance with the classical view. During the Spermatophyta evolution, the gymnosperms (Cycas, Ginkgo, and Metasequoia have been studied here) and the angiosperms (flowering plants) separated, and this was followed by the separation of Metasequoia and Cycas (cycad)/Ginkgo (maidenhair tree) on one branch and various flowering plants on the other. PMID:16593540

  8. Atmospheric Visual and Infrared Transmission Deduced from Surface Weather Observations: Weather and Warplanes. V1

    DTIC Science & Technology

    1976-10-01

    who provided support are H. H. Bailey, E. S. Batten, D. Deirmendjian, L. R. Koenig, L. G. Mundie and C. Schutz. The author also wishes to express...28 Appendix A. TARGET DETECTION MODELING EQUATIONS (AFTER BAILEY AND MUNDIE ) ............................................ 29...Bailey- Mundie [1,2] visual and infrared target acquisition nodels that have been adapted for u!:e with the WETTA algorithms and applied to several

  9. Petrographically deduced triassic climate for the Deep River Basin, eastern piedmont of North Carolina

    SciTech Connect

    McCarn, S.T.; Mansfield, C.F.

    1985-01-01

    A petrographic comparison of Triassic, fluvial sandstones from the Deep River Basin in the eastern piedmont of North Carolina with nearby Holocene stream sands (1) indicates that he Triassic climate was more arid than today's and (2) distinguishes an eastern, more plutonic terrane from a western, more metamorphic source terrane. The paleoclimatic interpretation is based on differences in framework composition between modern and ancient sands of the same grain size, derived from the same rock type, transported similar distances and deposited in similar settings. The Triassic sandstones contain more lithic-fragments but less quartz than otherwise equivalent, modern sand in the Deep River Basin. Feldspar content is more complex, controlled by both source-rock composition and climate. Sand from the more plutonic terrane contains more feldspar and plutonic lithic-fragments than sand from the more metamorphic terrane, which contains more quartz and metamorphic lithic-fragments. This petrographic interpretation of the Triassic sandstones along with the presence of coal, limestone, chert and caliche in the middle of the section suggests that the Triassic climate was cyclic, changing from arid to humid and back to arid. Plate-tectonic reconstructions place the Deep River Basin between the Triassic equator and Tropic of cancer, where the easterly trade winds would predominate. Therefore, the arid portions of the cycle could have been due to a periodic, orographic, rain shadow formed as the result of intermittent movement along the Jonesboro Fault, creating a highland area east of the Deep River Basin.

  10. Crustal structure of the Hecataeus Rise (eastern Mediterranean) deduced by marine gravity and marine magnetic modelling

    NASA Astrophysics Data System (ADS)

    Dehghani, Ali

    2016-04-01

    In the year 2010 extensive geophysical researches were carried out in the area of Hecataeus Rise using the German research vessel Maria S. Merian. Beside the bathymetry, refraction and reflection seismic data, marine gravity and marine magnetic data were acquired during this cruise. The result of the research along one Wide-Angle reflection/refraction seismic line of this cruise is published 2015 by K. Welford et al.. Based on interpretation of reflection seismic and bathymetry data across the Hecataeus Rise, S. Reiche published 2015 the crustal structure and bathymetric features along some seismic profiles of this cruise. The focus of this work is to use the available sediments and crustal structures inferred by seismic information together with real marine gravity and marine magnetic data in order to produce gravity and magnetic 2-D models along all seismic profiles. While Welford et al. used the altimetry gravity data and magnetic data from EMAG3 database for their modelling, the real gravity and magnetic data measured exactly along the seismic profiles will be used in this work. The advantage of the real marine gravity and real marine magnetic data used for the modelling is that they have higher accuracy in the values as well as in the positions. Furthermore, Welford et al. calculated the gravity and Magnetic models along some seismic profiles, while in this work the result of gravity and magnetic modelling along all seismic profiles of this cruise will be presented. The marine gravity and marine magnetic data along all seismic profiles were recorded continuously. The accuracy of marine gravity data is about ± 1 mGal, while the accuracy of Marine magnetic data is in the range of ± 3 nT. The results of 2-D gravity and magnetic modelling will be presented and discussed in this work.

  11. Rapid dewatering of the crust deduced from ages of mesothermal gold deposits

    USGS Publications Warehouse

    Goldfarb, R.J.; Snee, L.W.; Miller, L.D.; Newberry, R.J.

    1991-01-01

    The large-scale migration of fluids through the continental crust has been well documented, but there is no consensus regarding the timing of fluid migration relative to erogenic episodes, or rates of crustal dewatering1. Here we present 40Ar/39Ar dates for muscovites from quartz veins along a major shear zone in southeast Alaska, which show that the veins were emplaced in the early Eocene, during the late stages of orogenic deformation. Hydrothermal activity took place for only about 1 Myr and along a distance of at least 200 km. The fluids were generated by metamorphic reactions in subducted crust along the North American plate margin, and were apparently trapped in the crust by the low permeabilities accompanying a convergent tectonic regime until 56 Myr ago. The rapid dewatering event coincided with a change in plate motion at 56-55 Myr, which caused a shift from convergent to partly transcurrent tectonics. We suggest that this change in tectonic regime led to increased crustal permeabilities and hence the possibility of large-scale fluid migration.

  12. Inner ear anatomy is a proxy for deducing auditory capability and behaviour in reptiles and birds.

    PubMed

    Walsh, Stig A; Barrett, Paul M; Milner, Angela C; Manley, Geoffrey; Witmer, Lawrence M

    2009-04-07

    Inferences of hearing capabilities and audition-related behaviours in extinct reptiles and birds have previously been based on comparing cochlear duct dimensions with those of living species. However, the relationship between inner-ear bony anatomy and hearing ability or vocalization has never been tested rigorously in extant or fossil taxa. Here, micro-computed tomographic analysis is used to investigate whether simple endosseous cochlear duct (ECD) measurements can be fitted to models of hearing sensitivity, vocalization, sociality and environmental preference in 59 extant reptile and bird species, selected based on their vocalization ability. Length, rostrocaudal/mediolateral width and volume measurements were taken from ECD virtual endocasts and scaled to basicranial length. Multiple regression of these data with measures of hearing sensitivity, vocal complexity, sociality and environmental preference recovered positive correlations between ECD length and hearing range/mean frequency, vocal complexity, the behavioural traits of pair bonding and living in large aggregations, and a negative correlation between ECD length/rostrocaudal width and aquatic environments. No other dimensions correlated with these variables. Our results suggest that ECD length can be used to predict mean hearing frequency and range in fossil taxa, and that this measure may also predict vocal complexity and large group sociality given comprehensive datasets.

  13. Physical properties of aerosols in Titan's atmosphere as deduced from visible observations

    NASA Technical Reports Server (NTRS)

    Rages, K.; Pollack, J. B.

    1978-01-01

    Analysis of the absolute value of Titan's albedo and its variation with increasing phase angle has yielded constraints on the optical properties and average particle size of the aerosols responsible for the scattering of visible light. The real index of refraction of the scattering material lies within the range 1.5 approximately less than nr approximately less than 2.0 and the average particle size is somewhere between 0.2 micrometer and 0.4 micrometer. The amount of limb darkening produced by these models leads to an occultation radius of approximately 2700 km.

  14. Heterogeneity in maize starch granule internal architecture deduced from diffusion of fluorescent dextran probes.

    PubMed

    Dhital, Sushil; Shelat, Kinnari J; Shrestha, Ashok K; Gidley, Michael J

    2013-04-02

    Heterogeneity in maize starch granules was investigated by studying the diffusion of fluorescent dextran probes (20, 70 and 150kDa) inside granules using fluorescence recovery after photobleaching combined with confocal microscopy. Access of probes to the interior of granules was greatly enhanced by limited (2.4%) amylolysis. The diffusion of probes within granules was found to be either 'fast' with diffusion coefficients in the order of 10(-6)cm(2)s(-1) or 'slow' with diffusion coefficients in the order of 10(-7)cm(2)s(-1), independent of the size of dextran probes or prior treatment of the granules by α-amylase. Results were compared with observations of pores and channels in granules by electron microscopy and by confocal microscopy after labelling with 8-amino-1,3,6-pyrenetrisulfonic acid. It is proposed that there is an inherent heterogeneity of internal architecture in maize starch granules due to the presence or absence in individual granules of (a) pores leading to a central cavity, resulting in 'fast' diffusion of dextran probes and (b) accessibility of the starch polymer matrix to dextran probes, leading to 'slow' diffusion behaviour. The observed heterogeneity of maize starch granule porosity has implications for chemical modification reactions and the kinetics of digestion with amylases. Copyright © 2012 Elsevier Ltd. All rights reserved.

  15. A Model for Carbohydrate Metabolism in the Diatom Phaeodactylum tricornutum Deduced from Comparative Whole Genome Analysis

    PubMed Central

    Kaplan, Aaron; Caron, Lise; Weber, Till; Maheswari, Uma; Armbrust, E. Virginia; Bowler, Chris

    2008-01-01

    Background Diatoms are unicellular algae responsible for approximately 20% of global carbon fixation. Their evolution by secondary endocytobiosis resulted in a complex cellular structure and metabolism compared to algae with primary plastids. Methodology/Principal Findings The whole genome sequence of the diatom Phaeodactylum tricornutum has recently been completed. We identified and annotated genes for enzymes involved in carbohydrate pathways based on extensive EST support and comparison to the whole genome sequence of a second diatom, Thalassiosira pseudonana. Protein localization to mitochondria was predicted based on identified similarities to mitochondrial localization motifs in other eukaryotes, whereas protein localization to plastids was based on the presence of signal peptide motifs in combination with plastid localization motifs previously shown to be required in diatoms. We identified genes potentially involved in a C4-like photosynthesis in P. tricornutum and, on the basis of sequence-based putative localization of relevant proteins, discuss possible differences in carbon concentrating mechanisms and CO2 fixation between the two diatoms. We also identified genes encoding enzymes involved in photorespiration with one interesting exception: glycerate kinase was not found in either P. tricornutum or T. pseudonana. Various Calvin cycle enzymes were found in up to five different isoforms, distributed between plastids, mitochondria and the cytosol. Diatoms store energy either as lipids or as chrysolaminaran (a β-1,3-glucan) outside of the plastids. We identified various β-glucanases and large membrane-bound glucan synthases. Interestingly most of the glucanases appear to contain C-terminal anchor domains that may attach the enzymes to membranes. Conclusions/Significance Here we present a detailed synthesis of carbohydrate metabolism in diatoms based on the genome sequences of Thalassiosira pseudonana and Phaeodactylum tricornutum. This model provides novel insights into acquisition of dissolved inorganic carbon and primary metabolic pathways of carbon in two different diatoms, which is of significance for an improved understanding of global carbon cycles. PMID:18183306

  16. Grain Properties of Comet C/1995 O1 (Hale-Bopp) Deduced Through Computational Techniques

    NASA Technical Reports Server (NTRS)

    Harker, David E.; Wooden, Diane H.; Woodward, Charles E.; Lisse, Carey M.

    2001-01-01

    We present the computational analysis of the 7.6 - 13.2 micrometer infrared (IR) spectrophotometry (R approximately equal to 120) of comet C/1995 O1 (Hale-Bopp) in conjunction with concurrent observations which extend the spectral energy distribution from the near-infrared to far-infrared wavelengths. The observations include temporal epochs pre-perihelion, (1996 October UT and 1997 February UT), near perihelion (1997 April UT), and postperihelion (1997 June UT). Through the computational modeling of small amorphous carbon, and crystalline and amorphous silicate grains in Hale-Bopp's coma, we find that as the comet approached perihelion, the grain size distribution (the Hanner modified power law) steepened (N = 3.4 pre-perihelion, to N = 3.7 near and post-perihelion) along with an increase in the fractal porosity of larger (greater than 1 micrometer) grains. The peak of the grain size distribution remained constant (ap = 0.2 micrometer) at each epoch. We attribute the emergence of the 9.3 micrometer peak near perihelion to crystalline orthopyroxeno grains released from inside the nucleus. Crystalline silicates (olivine and orthopyroxene) make up about 30% (by mass) of the submicron sized (less than 1 micrometer) dust grains in Hale-Bopp's coma during each epoch.

  17. Horizontal and vertical distribution of freshwater in the Arctic Ocean deduced from historical hydrochemistry

    NASA Astrophysics Data System (ADS)

    Kawai, M. Y.; Tanaka, N.; Pivovarov, S.; Timokhov, L.

    2004-12-01

    Historical data (1935-2002) of two chemical tracers, oxygen isotope ratio and alkalinity, are combined and freshwater sources of sea ice meltwater (SIM), and other freshwater (OF) are distinguished from each pair of two conservative elements, salinity-oxygen isotope and salinity-alkalinity. The OF consists of mainly river runoff, with addition of precipitation and salinity deficit of inflowing Pacific water. Results give at first startling pictures of both horizontal and vertical distributions of freshwaters from the two designated sources almost throughout the Arctic Ocean. River water sources in Canada Basin: Distribution of OF at the 10 depth shows that river water from the Russian shelf seas flows out of the shelf between the Mendeleyev and the Lomonosov Ridges and exits thorough western part of the Fram Strait. Part of this freshwater enters into the Canadian Basin, where water contains large amount of OF but small fraction of American river water. This may suggest that river water from American continent should exit from the basin relatively fast though the Canadian Archipelago. Vertical mixing of fresh water: The atmospheric cooling and brine rejection during winter converts fresh surface water into denser water with high OF and negative SIM signals. High inventories of both OF and negative SIM, which implies mixing with brine, are found in the Canadian Basin, Baffin Bay and east of Novaya Zemlya in the Kara Sea. These signals in the Kara Sea and the Baffin Bay show occurrence of deep convection in winter, whereas those in the Canadian Basin come from adjacent shallow shelves. In the Canadian Basin, negative SIM distribution is well corresponding to the low temperature layer between surface and Atlantic origin waters. This layer characterized by nutrient maximum with a salinity of about 33.1 and is found to form from the water having salinity of 31-32. Our analysis advocates the importance of shelf water from the East Siberian Sea on formation of water mass in this layer. The shelf water vertically transports both OF and brine from the surface to the deeper layer and accumulates in the Canadian Basin. We appreciate Drs. Murata and Shimada of JAMSTEC for providing unpublished data from the past Arctic expeditions by R/V Mirai.

  18. Deducing the distribution of terminal electron-accepting processes in hydrologically diverse groundwater systems

    USGS Publications Warehouse

    Chapelle, Francis H.; McMahon, Peter B.; Dubrovsky, Neil M.; Fujii, Roger F.; Oaksford, Edward T.; Vroblesky, Don A.

    1995-01-01

    The distribution of microbially mediated terminal electron-accepting processes (TEAPs( was investigated in four hydrologically diverse groundwater systems by considering patterns of electron acceptor (nitrate, sulfate) consumption, intermediate product (hydrogen (H2)) concentrations, and final product (ferrous iron, sulfide, and methane) production. In each hydrologic system a determination of predominant TEAPs could be arrived at, but the level of confidence appropriate for each determination differed. In a portion of the lacustrine aquifer of the San Joaquin Valley, for example, all three indicators (sulfate concentrations decreasing, H2concentrations in the 1–2 nmol range, and sulfide concentrations increasing along flow paths identified sulfate reduction as the predominant TEAP, leading to a high degree of confidence in the determination. In portions of the Floridan aquifer and a petroleum hydrocarbon-contaminated aquifer, sulfate reduction and methanogenesis are indicated by production of sulfide and methane, and hydrogen oncentrations in the 1–4 nmol and 5–14 nmol range, respectively. However, because electron acceptor consumption could not be documented in these systems, less confidence is warranted in the TEAP determination. In the Black Creek aquifer, no pattern of sulfate consumption and sulfide production were observed, but H2 concentrations indicated sulfate reduction as the predominant TEAP. In this case, where just a single line of evidence is available, the least confidence in the TEAP diagnosis is justified. Because this methodology is based on measurable water chemistry parameters and upon the physiology of microbial electron transfer processes, it provides a better description of predominant redox processes in groundwater systems than more traditional Eh-based methods.

  19. Climate stability as deduced from an idealized coupled atmosphere-ocean model

    SciTech Connect

    Tang, B.; Weaver, A.J.

    1995-04-01

    The stability of an idealized climate system is investigated using a simple coupled atmosphere-ocean box model. Motivated by the results from general circulation models. the main physical constraint imposed on the system is that the net radiation at the top of the atmosphere is fixed. The specification of an invariant equatorial atmospheric temperature. Consistent with paleoclimatic data, allows the hydrological cycle to be internally determined from the poleward heat transport budget, resulting in a model that has a plausible representation of the hydrological cycle-thermohaline circulation interaction. The model suggests that the stability and variability of the climate system depends fundamentally on the mean climatic state (total heat content of the system). When the total heat content of the climate system is low, a stable present-day equilibrium exists with high-latitude sinking. Conversely, when the total heat content is high, a stable equatorial sinking equilibrium exists. For a range of intermediate values of the total heat content, internal climatic oscillations can occur through a hydrological cycle-thermohaline circulation feedback process. Experiments conducted with the model reveal that under a 100-year 2xCO{sub 2} warming, the thermohaline circulation first collapses but then recovers. Under a 100-year 4 x CO{sub 2} warming, the thermohaline circulation collapses and remains collapsed. Recent paleoclimatic data suggest that the climate system may behave very differently for warmer climate. Our results suggest that this may be attributed to the enhanced poleward freshwater transport, which causes increased instability of the present-day thermohaline circulation. 50 refs., 4 figs., 1 tab.

  20. Cenozoic marine geochemistry of thallium deduced from isotopic studies of ferromanganese crusts and pelagic sediments

    USGS Publications Warehouse

    Rehkamper, M.; Frank, M.; Hein, J.R.; Halliday, A.

    2004-01-01

    Cenozoic records of Tl isotope compositions recorded by ferromanganese (Fe-Mn) crusts have been obtained. Such records are of interest because recent growth surfaces of Fe-Mn crusts display a nearly constant Tl isotope fractionation relative to seawater. The time-series data are complemented by results for bulk samples and leachates of various marine sediments. Oxic pelagic sediments and anoxic marine deposits can be distinguished by their Tl isotope compositions. Both pelagic clays and biogenic oozes are typically characterized by ??205Tl greater than +2.5, whereas anoxic sediments have ??205Tl of less than -1.5 (??205Tl is the deviation of the 205Tl/203Tl isotope ratio of a sample from NIST SRM 997 Tl in parts per 104). Leaching experiments indicate that the high ??205Tl values of oxic sediments probably reflect authigenic Fe-Mn oxyhydroxides. Time-resolved Tl isotope compositions were obtained from six Fe-Mn crusts from the Atlantic, Indian, and Pacific oceans and a number of observations indicate that these records were not biased by diagenetic alteration. Over the last 25 Myr, the data do not show isotopic variations that significantly exceed the range of Tl isotope compositions observed for surface layers of Fe-Mn crusts distributed globally (??205 Tl=+12.8??1.2). This indicates that variations in deep-ocean temperature were not recorded by Tl isotopes. The results most likely reflect a constant Tl isotope composition for seawater. The growth layers of three Fe-Mn crusts that are older than 25 Ma show a systematic increase of ??205Tl with decreasing age, from about +6 at 60-50 Ma to about +12 at 25 Ma. These trends are thought to be due to variations in the Tl isotope composition of seawater, which requires that the oceans of the early Cenozoic either had smaller output fluxes or received larger input fluxes of Tl with low ??205Tl. Larger inputs of isotopically light Tl may have been supplied by benthic fluxes from reducing sediments, rivers, and/or volcanic emanations. Alternatively, the Tl isotope trends may reflect the increasing importance of Tl fluxes to altered ocean crust through time. ?? 2004 Elsevier B.V. All rights reserved.

  1. The verification of lightning location accuracy in Finland deduced from lightning strikes to trees

    NASA Astrophysics Data System (ADS)

    Mäkelä, Antti; Mäkelä, Jakke; Haapalainen, Jussi; Porjo, Niko

    2016-05-01

    We present a new method to determine the ground truth and accuracy of lightning location systems (LLS), using natural lightning strikes to trees. Observations of strikes to trees are being collected with a Web-based survey tool at the Finnish Meteorological Institute. Since the Finnish thunderstorms tend to have on average a low flash rate, it is often possible to identify from the LLS data unambiguously the stroke that caused damage to a given tree. The coordinates of the tree are then the ground truth for that stroke. The technique has clear advantages over other methods used to determine the ground truth. Instrumented towers and rocket launches measure upward-propagating lightning. Video and audio records, even with triangulation, are rarely capable of high accuracy. We present data for 36 quality-controlled tree strikes in the years 2007-2008. We show that the average inaccuracy of the lightning location network for that period was 600 m. In addition, we show that the 50% confidence ellipse calculated by the lightning location network and used operationally for describing the location accuracy is physically meaningful: half of all the strikes were located within the uncertainty ellipse of the nearest recorded stroke. Using tree strike data thus allows not only the accuracy of the LLS to be estimated but also the reliability of the uncertainty ellipse. To our knowledge, this method has not been attempted before for natural lightning.

  2. A source of CO2 to the atmosphere throughout the year in the Maranhense continental shelf (2°30'S, Brazil)

    NASA Astrophysics Data System (ADS)

    Lefèvre, Nathalie; da Silva Dias, Francisco Jose; de Torres, Audálio Rebelo; Noriega, Carlos; Araujo, Moacyr; de Castro, Antonio Carlos Leal; Rocha, Carlos; Jiang, Shan; Ibánhez, J. Severino P.

    2017-06-01

    To reduce uncertainty regarding the contribution of continental shelf areas in low latitude regions to the air-sea CO2 exchange, more data are required to understand the carbon turnover in these regions and cover gaps in coverage. For the first time, inorganic carbon and alkalinity were measured along a cross-shelf transect off the coast of Maranhão (North Brazil) in 9 cruises spawning from April 2013 to September 2014. On the last 4 transects, dissolved organic matter and nutrients were also measured. The highest inorganic and organic carbon concentrations are observed close to land. As a result of low productivity and significant remineralization, heterotrophy dominates along the transect throughout the year. Although the temporal variability is significantly reduced at the offshore station with carbon concentrations decreasing seaward, the fugacity of CO2 (fCO2) at this station remains significantly higher, especially during the wet season, than the open ocean values measured routinely by a merchant ship further west. Overall, the continental shelf is a weak source of CO2 to the atmosphere throughout the year with an annual mean flux of 1.81±0.84 mmol m-2 d-1. The highest magnitudes of fCO2 are observed during the wet season when the winds are the weakest. As a result, the CO2 flux does not show a clear seasonal pattern. Further offshore, fCO2 is significantly lower than on the continental shelf. However, the oceanic CO2 flux, with an annual mean of 2.32±1.09 mmol m-2 d-1, is not statistically different from the CO2 flux at the continental shelf because the wind is stronger in the open ocean.

  3. No strong evidence for increased risk of breast cancer 8-26 years after multiple mammograms in their 30s in females at moderate and high familial risk.

    PubMed

    Evans, D Gareth; Kotre, C John; Harkness, Elaine; Wilson, Mary; Maxwell, Anthony J; Howell, Anthony

    2016-01-01

    To assess the risks of induction of breast tumours from frequent screening mammography in younger females. A study group of 853 females was identified who had at least 5 mammograms starting before 37 years of age, with 4 or more before the age of 40 years. These were followed up from their 40th birthday or 8 years from their first mammogram, and their cancer incidence was compared with that of a control group of 1103 females who had an average of 5 mammograms between the ages of 40 and 46 years. All females in the study were previously assessed to be at moderate familial risk or higher. There were 43 incident breast cancers in the study group after the 8-year start point, whereas 38.3 were expected from life-table calculations (RR 1.12; 95% CI: 0.83 to 1.51). In the control group, 50 incident breast cancers developed some time after their first mammogram in follow up to age 60 years. The observed, expected ratio from life tables in this group was 0.94 (95% CI: 0.71-1.24), similar to that in the study group. There was no trend to greater cancer incidence in those receiving mammograms earlier. This study shows that there is no substantial effect on the induction of additional primary breast tumours from frequent mammography starting at <37 years of age. Further work on larger numbers of females is necessary to assess longer term risks and determine whether a small excess cancer effect may be present.

  4. Quinine, Malaria, and the Cinchona Bureau: Marketing Practices and Knowledge Circulation in a Dutch Transoceanic Cinchona-Quinine Enterprise (1920s-30s).

    PubMed

    Van Der Hoogte, Arjo Roersch; Pieters, Toine

    2016-04-01

    In this study, we will show how a Dutch pharmaceutical consortium of cinchona producers and quinine manufacturers was able to capitalize on one of the first international public health campaigns to fight malaria, thereby promoting the sale of quinine, an antimalarial medicine. During the 1920s and 1930s, the international markets for quinine were controlled by this Dutch consortium, which was a transoceanic cinchona-quinine enterprise centered in the Cinchona Bureau in the Netherlands. We will argue that during the interwar period, the Cinchona Bureau became the decision-making center of this Dutch cinchona-quinine pharmaceutical enterprise and monopolized the production and trade of an essential medicine. In addition, we will argue that capitalizing on the international public health campaign in the fight against malaria by the Dutch cinchona-quinine enterprise via the Cinchona Bureau can be regarded as an early example of corporate colonization of public health by a private pharmaceutical consortium. Furthermore, we will show how commercial interests prevailed over scientific interests within the Dutch cinchona-quinine consortium, thus interfering with and ultimately curtailing the transoceanic circulation of knowledge in the Dutch empire.

  5. Quinine, Malaria, and the Cinchona Bureau: Marketing Practices and Knowledge Circulation in a Dutch Transoceanic Cinchona–Quinine Enterprise (1920s–30s)

    PubMed Central

    Roersch Van Der Hoogte, Arjo; Pieters, Toine

    2016-01-01

    In this study, we will show how a Dutch pharmaceutical consortium of cinchona producers and quinine manufacturers was able to capitalize on one of the first international public health campaigns to fight malaria, thereby promoting the sale of quinine, an antimalarial medicine. During the 1920s and 1930s, the international markets for quinine were controlled by this Dutch consortium, which was a transoceanic cinchona–quinine enterprise centered in the Cinchona Bureau in the Netherlands. We will argue that during the interwar period, the Cinchona Bureau became the decision-making center of this Dutch cinchona–quinine pharmaceutical enterprise and monopolized the production and trade of an essential medicine. In addition, we will argue that capitalizing on the international public health campaign in the fight against malaria by the Dutch cinchona–quinine enterprise via the Cinchona Bureau can be regarded as an early example of corporate colonization of public health by a private pharmaceutical consortium. Furthermore, we will show how commercial interests prevailed over scientific interests within the Dutch cinchona–quinine consortium, thus interfering with and ultimately curtailing the transoceanic circulation of knowledge in the Dutch empire. PMID:26054829

  6. In Your 20s it’s Quantity, in Your 30s it’s Quality: The Prognostic Value of Social Activity Across 30 Years of Adulthood

    PubMed Central

    Carmichael, Cheryl L.; Reis, Harry T.; Duberstein, Paul R.

    2015-01-01

    Social connection, a leading factor in the promotion of health, well-being, and longevity, requires social knowledge and the capacity to cultivate intimacy. Lifespan development theorists have speculated that social information-seeking goals, emphasized at the beginning of early adulthood, give way to emotional closeness goals in later stages of early adulthood. Drawing on developmental theory (Baltes, 1997; Baltes & Carstensen, 2003), this 30-year prospective study assessed social activity at age-20 and age-30 with experience sampling methods, and psychosocial outcomes (social integration, friendship quality, loneliness, depression, and psychological well-being) at age-50. Results supported the hypothesis that the quantity (but not the quality) of social interactions at age-20, and the quality (but not the quantity) of social interactions at age-30 predict midlife psychosocial outcomes. Longitudinal structural models revealed that age-20 interaction quantity had a direct, unmediated effect on age-50 social and psychological outcomes. The effects of age-20 interaction quality on midlife outcomes, on the other hand, were mediated by age-30 interaction quality. Our findings are consistent with the idea that selection and optimization serve important functions in early adulthood, and that engaging in developmentally appropriate social activity contributes to psychosocial adjustment in the decades that follow. PMID:25774426

  7. In your 20s it's quantity, in your 30s it's quality: the prognostic value of social activity across 30 years of adulthood.

    PubMed

    Carmichael, Cheryl L; Reis, Harry T; Duberstein, Paul R

    2015-03-01

    Social connection, a leading factor in the promotion of health, well-being, and longevity, requires social knowledge and the capacity to cultivate intimacy. Life span development theorists have speculated that social information-seeking goals, emphasized at the beginning of early adulthood, give way to emotional closeness goals in later stages of early adulthood. Drawing on developmental theory (Baltes & Carstensen, 2003; Baltes, 1997), this 30-year prospective study assessed social activity at age 20 and age 30 with experience sampling methods, and psychosocial outcomes (social integration, friendship quality, loneliness, depression, and psychological well-being) at age 50. Results supported the hypothesis that the quantity (but not the quality) of social interactions at age 20, and the quality (but not the quantity) of social interactions at age 30 predict midlife psychosocial outcomes. Longitudinal structural models revealed that age-20 interaction quantity had a direct, unmediated effect on age-50 social and psychological outcomes. The effects of age-20 interaction quality on midlife outcomes, on the other hand, were mediated by age-30 interaction quality. Our findings are consistent with the idea that selection and optimization serve important functions in early adulthood, and that engaging in developmentally appropriate social activity contributes to psychosocial adjustment in the decades that follow.

  8. Effect of individual time to peak power output on the expression of peak power output in the 30-s Wingate Anaerobic Test.

    PubMed

    Bell, W; Cobner, D M

    2007-02-01

    The objective of the present investigation was to examine a proposal which stated that individual power values should be aligned according to peak power output (PPO) before calculating the mean value of PPO. This procedure removes the variation in time it takes for individuals to reach PPO. Participants were forty-one University Rugby Union Football players of mean age 21.7 +/- 2.6 years, height 181.4 +/- 6.9 cm and body mass 88.9 +/- 12.7 kg. Data were collected using a friction-belt cycle ergometer (Monark 864, Varberg, Sweden). A significantly larger mean value for PPO was found when results were calculated from time-aligned rather than cross-sectional data (1154 +/- 246 vs. 1121 +/- 254 W, p < 0.0001); the mean difference was approximately 3 %. Additionally, the average profile of the power output curve was more reflective of individual power curves. The negative correlation between PPO and the time taken to reach PPO was - 0.32 (p < 0.05), confirming the view that the earlier the time taken to reach PPO the larger the PPO. It was concluded that the mean value of PPO and the corresponding profile for power output curves are best represented by the analysis of time-aligned rather than cross-sectional data.

  9. Ultramafic and mafic rocks from the Garret Transform Fault near 13°30'S on the East Pacific Rise: Igneous petrology

    NASA Astrophysics Data System (ADS)

    Hébert, Réjean; Bideau, Daniel; Hekinian, Roger

    1983-10-01

    Serpentinized peridotites, metagabbros and fresh basaltic rocks were dredged from the Garret Transform Fault near 13°S on the East Pacific Rise. Two dredge hauls taken on the northern wall (from about 3100-3600 m) consisted of aphyric and picritic basalts; while peridotites, gabbros and moderately phyric basalts were recovered in a single dredge located near the deepest part (4616-4820 m) of the transform valley. Well preserved igneous textures and mineralogical assemblages enable us to differentiate between tectonites (including harzburgites and clinopyroxene-bearing harzburgites) and cumulates (consisting of plagioclase-dunite, troctolites, olivine-gabbros and ilmenite-gabbros). The harzburgites are likely to represent deep-seated peridotites left after extraction of basaltic melt during upper mantle partial melting of a lherzolite. The ultramafic cumulates underwent some deformation and show textural and mineralogical evidences which suggest formation at a minimum depth corresponding to the very lower crust. It is also inferred that the composition of the coexisting liquid along with early cumulus crystals has a ratio Mg/(Mg + Fe 2+) of 0.7, high CaO/Na 2O ratios and a low Ni content (about 150 ppm) when compared to similar rock types from Atlantic fracture zones. Subsequent uplift during rifting of the oceanic lithosphere enhanced plastic deformation, subsolidus recrystallization and retrogressive metamorphism of the gabbros and the ultramafics.

  10. Pushing up the size limit of chalcogenide supertetrahedral clusters: two- and three-dimensional photoluminescent open frameworks from (Cu(5)In(30)S(54))(13-) clusters.

    PubMed

    Bu, Xianhui; Zheng, Nanfeng; Li, Yuqi; Feng, Pingyun

    2002-10-30

    Direct band gap copper indium chalcogenides are of great technological importance in part because of their high photovoltaic conversion efficiency. Covalent superlattices constructed from copper indium chalcogenide clusters are of particular interest because they may combine open framework architecture with semiconducting properties. Here two photoluminescent covalent superlattices built from core-shell type copper indium sulfide supertetrahedral clusters are reported. Each cluster consists of 35 metal cations and is so far the largest known supertetrahedral cluster with a metal-to-metal distance of 1.6 nm. In addition, this is the first example of supertetrahedral clusters in heterometallic copper indium chalcogenides. The preparation of these large clusters has narrowed down the size gap between colloidal nanoclusters and small supertetrahedral clusters and revealed new possibilities in the construction of nanoporous semiconducting superlattices with tunable pore size. Through the combination of metal ions with different oxidation states to provide both overall and local charge neutrality, an effective approach has been demonstrated in the rational synthesis of chalcogenide open framework materials with large and unprecedented supertetrahedral clusters.

  11. Climate change in the Turkana basin as deduced from a 4000 year long δO 18 record

    NASA Astrophysics Data System (ADS)

    Ricketts, R. D.; Johnson, T. C.

    1996-07-01

    A model of the oxygen isotopic composition of Lake Turkana waters sheds insight into the paleoclimatic interpretation of a δ 18O profile of inorganic calcite from a core taken from the lake. The model indicates that high frequency fluctuations seen in the isotopic record are probably due to shifts in lake level, while the long term increase of 1.4‰ seen from 4 to 2 kyr BP cannot be due solely to lake level change. It must be due to changes in other climatic parameters, such as the isotopic composition of the inflow. These findings suggests that the abrupt change observed in Lake Turkana (and many other African lakes) at 4 ka was the precursor to a 2000 year long adjustment in the climate over the Ethiopian Plateau. The isotopic record from Lake Turkana agrees with other paleoclimate records from the basin, including the diatom stratigraphy and radiocarbon dates on exposed shoreline deposits.

  12. Sources of Educational Information: European Survey, 1984=Sources d'information en matiere d'education: Enquete europeenne, 1984.

    ERIC Educational Resources Information Center

    Council of Europe, Strasbourg (France). Documentation Center for Education in Europe.

    A survey was conducted to update a series of documents published by the Council of Europe in 1975. This survey covers the following countries: Austria, Belgium (Dutch-speaking area), Cyprus, Denmark, Finland, Iceland, Ireland, Liechtenstein, Luxembourg, Switzerland, and the United Kingdom. For each country, details are given concerning sources of…

  13. Single-Path Sigma and Spatial Correlation of Gmpe Residuals Deduced from a Huge Dataset in Taiwan

    NASA Astrophysics Data System (ADS)

    Sung, C. H.; Lee, C. T.; Gao, J. C.

    2014-12-01

    A strong ground-motion attenuation equation is an essential part in the probability seismic hazard analysis. Therefore, we need to study the strong ground-motion attenuation relationship for earthquakes in Taiwan. In this study, well processed strong ground-motion data from aiwan Strong Motion Instrumentation Program (TSMIP) in Taiwan are used to establish attenuation equations. A total of 158 earthquakes with 18,859 records are selected and nonlinear mixed-effect model with maximum likelihood method is used to accomplish the regression analysis of ground-motion attenuation relationships for peak ground acceleration (PGA), with considering earthquake source, distance, site condition (Vs30), fault mechanism, and tectonic regine. The very much concerned ground-motion variability in PSHA is reviewed and studied with huge amount of data from Taiwan. We analyzed aleatory variability firstly by direct observation on inter-event variability and site-specific variability, and then by two additional simple approaches, the variogram and the standard deviation distribution in single site stady and in single earthquake study and got spatial distribution of single-path sigma as well as their earthquake component and site component. The single-path sigma is ranging from 0.219 to 0.254. These are 58% to 64% smaller than the total sigma (0.601). If we only use aleatory variability in PSHA, then the resultant hazard level would be 20% lower than the traditional one in 2,475 year return-perid.

  14. Gamow-Teller Strengths in Proton-Rich Exotic Nuclei Deduced in the Combined Analysis of Mirror Transitions

    SciTech Connect

    Fujita, Y.; Adachi, T.; Fujita, H.; Shimbara, Y.; Brentano, P. von; Fransen, C.; Pietralla, N.; Zell, K.O.; Berg, G.P.A.; Frenne, D. de; Jacobs, E.; Negret, A.; Popescu, L.; Fujita, K.; Hatanaka, K.; Nakanishi, K.; Sakemi, Y.; Shimizu, Y.; Tameshige, Y.; Tamii, A.

    2005-11-18

    Isospin symmetry is expected for the T{sub z}={+-}1{yields}0 isobaric analogous transitions in isobars with mass number A, where T{sub z} is the z component of isospin T. Assuming this symmetry, strengths of analogous Gamow-Teller (GT) transitions within A=50 isobars were determined from a high energy-resolution T{sub z}=+1{yields}0, {sup 50}Cr({sup 3}He,t){sup 50}Mn study at 0 deg. in combination with the decay Q value and lifetime from the T{sub z}=-1{yields}0, {sup 50}Fe{yields}{sup 50}Mn {beta} decay. This method can be applied to other pf-shell nuclei and can be used to study GT strengths of astrophysical interest.

  15. Gamow-teller strengths in proton-rich exotic nuclei deduced in the combined analysis of mirror transitions.

    PubMed

    Fujita, Y; Adachi, T; von Brentano, P; Berg, G P A; Fransen, C; De Frenne, D; Fujita, H; Fujita, K; Hatanaka, K; Jacobs, E; Nakanishi, K; Negret, A; Pietralla, N; Popescu, L; Rubio, B; Sakemi, Y; Shimbara, Y; Shimizu, Y; Tameshige, Y; Tamii, A; Yosoi, M; Zell, K O

    2005-11-18

    Isospin symmetry is expected for the T(z)=+/-1-->0 isobaric analogous transitions in isobars with mass number A, where T(z) is the z component of isospin T. Assuming this symmetry, strengths of analogous Gamow-Teller (GT) transitions within A = 50 isobars were determined from a high energy-resolution study at 0 degrees in combination with the decay Q value and lifetime from the beta decay. This method can be applied to other pf-shell nuclei and can be used to study GT strengths of astrophysical interest.

  16. Empirical study of the metal-nitride-oxide-semiconductor device characteristics deduced from a microscopic model of memory traps

    SciTech Connect

    Ngai, K.L.; Hsia, Y.

    1982-07-15

    A graded-nitride gate dielectric metal-nitride-oxide-semiconductor (MNOS) memory transistor exhibiting superior device characteristics is presented and analyzed based on a qualitative microscopic model of the memory traps. The model is further reviewed to interpret some generic properties of the MNOS memory transistors including memory window, erase-write speed, and the retention-endurance characteristic features.

  17. Empirical study of the metal-nitride-oxide-semiconductor device characteristics deduced from a microscopic model of memory traps

    NASA Astrophysics Data System (ADS)

    Ngai, Kia L.; Hsia, Yukun

    1982-07-01

    A graded-nitride gate dielectric metal-nitride-oxide-semiconductor (MNOS) memory transistor exhibiting superior device characteristics is presented and analyzed based on a qualitative microscopic model of the memory traps. The model is further reviewed to interpret some generic properties of the MNOS memory transistors including memory window, erase-write speed, and the retention-endurance characteristic features.

  18. The pitfalls of deducing ethics from behavioral economics: why the Association of American Medical Colleges is wrong about pharmaceutical detailing.

    PubMed

    Huddle, Thomas S

    2010-01-01

    The Association of American Medical Colleges (AAMC) is urging academic medical centers to ban pharmaceutical detailing. This policy followed from a consideration of behavioral and neuroeconomics research. I argue that this research did not warrant the conclusions drawn from it. Pharmaceutical detailing carries risks of cognitive error for physicians, as do other forms of information exchange. Physicians may overcome such risks; those determined to do so may ethically engage in pharmaceutical detailing. Whether or not they should do so is a prudential judgment about which reasonable people may disagree. The AAMC's ethical condemnation of detailing is unwarranted and will subvert efforts to maintain a realm of physician discretion in clinical work that is increasingly threatened in our present practice environment.

  19. Two millennia of forest history deduced from closed depressions in the Lorrain plain (North-eastern, France)

    NASA Astrophysics Data System (ADS)

    Etienne, David; Ruffaldi, Pascale; Ritz, Frederic; Dupouey, Jean Luc; Dambrine, Etienne

    2010-05-01

    Recent archaeological surveys and ecological investigations in large "ancient" forests have shown that these areas had been often cultivated during the Roman or Medieval periods, and that this former land use is still deeply influencing present soil properties and plant biodiversity. This new perspective has boosted the research for sediment archives describing the state of forests across the archaeological and historical periods, especially in low altitude forest. Closed depressions (CD) or small hollows (over 30 000 CDs) are found in many silty plains of North-Western Europe (north-eastern France, Luxemburg and Belgium). They are defined as small (100 to 400 m²) closed wetlands, mostly supplied by rainwater. Their origin is debated. Recent coring campaigns in CDs of Lorraine (north-eastern France), 3 to 5 meters thick sediment cores were retrieved. It opened the way for palynological and pedological reconstruction of former landscapes. Here we present a sediment analysis of four peaty CDs (Assenoncourt, Römersberg, Sarrebourg and St Jean), located in different low altitude beech (Fagus) and oak (Quercus) forests, on silty clay soils, 50km from Nancy. As the oldest available map (Naudins, dated from 1728 to 1739) indicated forest boundaries similar to the present ones, these forests were considered as ancient forests. The sedimentation begins during the second Iron Age or Roman period. By this time, pollen analyses show an open landscape (70% of Non Arboreal Pollen), composed mostly by grassland (Plantago major/media, Poaceae and Asteraceae) and cropland (Cerealia-type, Centaurea cyanus). Around the 5th century AD, coinciding with the collapse of the Roman Empire, the pollen sequences describe rapid afforestation by Betula and Corylus, and later Carpinus forest. From the 8th century AD, Carpinus decreases in favour of Quercus which may reflect an anthropogenic clearing. From the 10th to the 14th century AD, croplands expand again with cultivation of hemp (Cannabis-type) and rye (Secale-type). From the 15th to the 19th century AD, pollen diagrams are similar at three sites and differ from the fourth. At Assenoncourt, St Jean and Römersberg, the contribution of Quercus, Carpinus and Fagus remains almost constant: 40%, 10% and 10%. This pattern may be related to short rotation forestry management applied in order to provide fuel wood to the local salt industry. At the fourth site (Sarrebourg), pollen assemblage varies with successive Quercus and Carpinus phases, following a natural sylvicultural evolution. Finally, the present-day forest extension took place during the 19th century with the replacement of wood by coal in the salt industry and the recent collapse of this salt industry during the 20th century. This study confirms, in the context of low altitude forests with heavy soils, what had been observed on shallow calcareous soils of the Lorrain plateau. Most of our state forests, that were thought to be "very ancient" or "immemorial" forest, have been managed for agriculture in the deep past. Because agriculture lands were often limed, fertilized, and eroded, this former agriculture use may to a large extent explain present soil properties and, as a consequence, present biodiversity.

  20. Interplate coupling, strain partitioning, and block motions deduced from GNSS and GPS/A data in southwest Japan

    NASA Astrophysics Data System (ADS)

    Nishimura, T.

    2016-12-01

    Contemporary deformation of the Japanese Islands is densely monitored by continuous GNSS networks including the GEONET. Recently geodetic network has expanded to offshore regions and clarify horizontal velocity near trench and trough axes. We present a result on estimating simultaneously interplate coupling on subduction megathrusts and rigid block rotations using a new geometry of tectonic blocks (e.g., microplates) and additional onshore and offshore data which are never used in previous studies (i.e., Loveless and Meade, 2008) in southwest Japan. We used horizontal and vertical velocity at 849 GNSS stations operated by the Geospatial Information Authority of Japan, Japan Coast Guard, Kyoto Univ., and IGS. We estimated interseismic velocities before the 2011 Tohoku-oki earthquake by fitting a linear function to daily GNSS coordinates estimated using GIPSY software. We also used 15 horizontal vectors at offshore GPS/Acoustic stations (Yokota et al., 2016). Surface velocity is assumed to be sum of rigid block rotations and elastic deformation due to locking on faults bounded rigid blocks in the block-fault model. We assumed to divide continental and oceanic plates into 15 and 3 blocks, respectively. We estimated them using a code of DEFNODE (McCaffrey, 2004). Geodetic coupling ratio on a subduction interface along the Nankai Trough shows high in the off-Shikoku and Off-Tokai areas(Fig. 1). In the area offshore the Kii Peninsula, coupling ratio is intermediate and its distribution is heterogeneous. A slip-deficit rate along the trough generally decreases toward east. We found coupling ratio is small along a most part of a trough axis, although uncertainties are still large despite using the offshore data. The San'in shear zone, the Median Tectonic Line, and the Niigata-Kobe Tectonic Zone accommodate 30% of relative motion between stable Amurian and Philippine Sea plates.

  1. The spatial and temporal distribution of lunar mare basalts as deduced from analysis of data for lunar meteorites

    NASA Astrophysics Data System (ADS)

    Basilevsky, A. T.; Neukum, G.; Nyquist, L.

    2010-12-01

    In this work we analyze data for lunar meteorites with emphasis on the spatial and temporal distribution of lunar mare basalts. The data are mostly from the Lunar Meteorite Compendium ( http://www-curator.jsc.nasa.gov/antmet/lmc/contents.cfm cited hereafter as Compendium) compiled by Kevin Righter, NASA Johnson Space Center, and from the associated literature. Analysis of the data showed that (i) a significant part of the lunar meteorite source craters are not larger than hundreds of meters in diameter; (ii) cryptomaria seem to be rather abundant in lunar highlands; (iii) the ratios of lunar meteorites belonging to three broad petrologic groups (mare basalt/gabbro, feldspatic highland breccias, and mingled breccias which are a mixture of mare and highland components) seem to be roughly proportional to the areal distribution of these rocks on the lunar surface; and (iv) the meteorite mare basalt ages show a range from ˜2.5 to 4.3 Ga and fill the gaps in the Apollo/Luna basalt age distribution. The ages of mare basalt clasts from mingled breccias seem to be systematically higher than those of "normal" mare basalts, which supports the suggestion that mingled breccias originated mostly from cryptomaria.

  2. Calcium translocation mechanism in sarcoplasmic reticulum vesicles, deduced from location studies of protein-bound spin labels.

    PubMed Central

    Champeil, P; Rigaud, J L; Gary-Bobo, C M

    1980-01-01

    Sarcoplasmic reticulum vesicles were exposed to various thiol-directed spin labels, and the position of the label on the inner or outer vesicle surface was investigated as a function of the ATPase (adenosinetriphosphatase; ATP phosphohydrolase, EC 3.6.1.3) chemical state. Previous measurements of label accessibility to externally added ascorbate had been considered to suggest an external-internal transition of protein-bound labels, coupled with ion translocation [Tonomura, Y. & Morales, M.F. (1974) Proc. Natl. Acad. Sci. USA 71, 3687-3691]. We show that these ascorbate studies do not lead to convincing conclusions. We demonstrate, on the contrary, that transition ions (nickel and ferricyanide) can be used as selective line-broadening agents for the signals arising from external labels. No significant difference in nickel- or ferricyanide-label interaction can be attributed to a different orientation of the label in any of the enzyme chemical states tested. Our results therefore contradict the current interpretation of ascorbate quenching experiments in terms of calcium ATPase rotatory motion; rather they are consistent with ion transport models involving only limited conformational rearrangements of the pump. PMID:6446710

  3. Large-eddy simulation and deduced scaling analysis of Rayleigh Bénard convection up to Ra = 109**

    NASA Astrophysics Data System (ADS)

    Peng, S.-H.; Hanjalic, K.; Davidson, L.

    Large-eddy simulation of turbulent Rayleigh Bénard (RB) convection has been performed for a 6:1:6 open-ended domain for Rayleigh numbers ranging from 6.3 × 105 to 109 at Prandtl number of Pr = 0.71. The scaling analysis based on the LES data shows that the heat transfer follows a single relation of Nu = 0.162 Ra0.286, which is consistent with the scaling law for the hard turbulence regime reported in several earlier experimental and DNS studies. The present LES also supports some earlier experimental and DNS findings that most of characteristic parameters can be scaled reasonably well with Ra number in the considered Ra number range using a single relation. Nonetheless, it is found that the scaling of several quantities shows a sensible offset from a single relation, and could be fitted better with the separate scaling relations for the soft and hard convective turbulence transitioned at about Ra = 4 × 107. It has been argued that the transition, reflected in the scaling relation, may be attributed to the increasing ‘containing effect’ of the plume leaving the horizontal wall on the plume approaching the wall at large Ra numbers in the near-wall region. **This paper is a modified version from the paper presented at the Forth International Symposium of Turbulence and Shear Flow Phenomena (Williamsburg, Virginia, 27 29 June 2005).

  4. Crustal thickening or isostatic rebound of orogenic wedge deduced from tectonostratigraphic units in Indosinian foreland basin, Longmen Shan, China

    NASA Astrophysics Data System (ADS)

    Li, Yong; Li, Haibing; Zhou, Rongjun; Su, Dechen; Yan, Liang; Yan, Zhaokun

    2014-04-01

    Longmen Shan is located at the boundary between the Sichuan Basin and Tibetan Plateau, representing the steepest gradient of any edges of the plateau. Three endmember models of uplift process and mechanism have been proposed, including crustal thickening, crustal flow, and crustal isostatic rebound. Here, we use tectonostratigraphic units in the Late Triassic foreland basin to restraint uplift process and mechanism in the Longmen Shan during Indosinian orogeny. The Late Triassic foreland basin developed as a flexural foredeep on the Yangtze passive continental margin during the Indosinian orogeny. The basin fill includes the Maantang Formation, the Xiaotangzi Formation and the Xujiahe Formation, and it is divided into four tectonostratigraphic units with wedge-shaped or tabular cross-sectional geometry by unconformities and flooding surfaces in this paper. The first and third ones are wedge-shaped tectonostratigraphic units and correspond to underfilled condition with basal unconformities or major flooding surfaces, the high rate of subsidence and sediment accumulation, coarsening-upward succession and a dual sediment supply, and them may link to strong active thrust loading events or the rapidly advance rate of the orogenic wedge; the second and fourth ones are tabular tectonostratigraphic units and correspond to overfilled condition with the unconformity, the low rate of subsidence and sediment accumulation, fining-upward succession, a single sediment supply, the mass emergence of conglomerate layers, and they may be related to isostatic rebound and erosion unloading. Two endmember models of uplift process and mechanism in the Longmen Shan during Indosinian cycle have been proposed by coeval Late Triassic sedimentary sequences in the foreland basin here: (1) crustal thickening during the wedge-shaped tectonostratigraphic units, (2) crustal isostatic rebound during the tabular tectonostratigraphic units. This two endmember models proposed in this paper may be helpful to understand the mechanism of the Wenchuan Earthquake.

  5. Detailed spatial distribution of microearthquakes beneath the Marmara Sea, Turkey, deduced from long-term ocean bottom observation

    NASA Astrophysics Data System (ADS)

    Yamamoto, Yojiro; Takahashi, Narumi; Pinar, Ali; Kalafat, Doǧan; Citak, Seckin; Comoglu, Mustafa; Polat, Remzi; Çok, Özkan; Ogutcu, Zafer; Suvariklı, Murat; Tunc, Suleyman; Gürbüz, Cemil; Turhan, Fatih; Ozel, Nurcan; Kaneda, Yoshiyuki

    2016-04-01

    The North Anatolian Fault (NAF) crosses the Marmara Sea in E-W direction, accommodating about 25 mm/yr of right-lateral motion between Anatolia and the Eurasian plate. There are many large earthquakes along the 1500 km long NAF repeatedly occurred and interacted each other. The recent large northern Aegean earthquake with Mw=6.9 filled one of the last two seismic gaps on NAF that experienced extraordinary seismic moment release c