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Sample records for 3b laboratory seal

  1. An Overview of the NASA P-3B Airborne Laboratory

    NASA Technical Reports Server (NTRS)

    Guillory, Anthony R.; Postell, George W.

    2009-01-01

    The National Aeronautics and Space Administration (NASA) Wallops Flight Facility (WFF) P-3B Orion is a medium-lift, four engine turbo-prop aircraft that has been reconfigured from a military aircraft to an Earth Science research platform. The aircraft has a long history of supporting science missions, flying on average over 200 hours per year. Examples of research missions that have been flown aboard the aircraft are remote sensing flights to study geophysical parameters including ice-sheet topography and periodic change, soil moisture content, atmospheric aerosol constituents, and beach erosion. Missions are conducted for the purposes of calibration/validation of various NASA and international satellites that monitor climate change as well as process studies and the test of new prototype remote sensing instruments. In recent y ears the focus has been on ice surveys of the Arctic and Antarctic, soil moisture research, and measurements of atmospheric chemistry and radiation sciences. The aircraft has been conducting ice surveys of Greenland since 1993 for the purposes of topographic mapping of both the surface and basal topography. Another application of the aircraft has been for soil moisture research. Research has also been conducted using microwave radiometers and radars over various agricultural and forest lands. Recently, a mission was flown in the spring over the High-Arctic to collect air samples of haze and boreal forest fires in an effort to determine anthropogenic amounts and sources of pollution. This pa per will provide an overview of the P-3B platform and highlight recent science missions.

  2. Seal Wire Integrity Verification Instrument: Evaluation of Laboratory Prototypes

    SciTech Connect

    Good, Morris S.; Skorpik, James R.; Kravtchenko, Victor; Wishard, Bernard; Prince, James M.; Pardini, Allan F.; Heasler, Patrick G.; Santiago-Rojas, Emiliano; Mathews, Royce; Khayyat, Sakher; Tanner, Jennifer E.; Undem, Halvor A.

    2009-10-07

    Tamper indicating devices (TIDs) provide evidence that sensitive items, to which they have been applied, have been tampered with or not. Passive wire-loop seals, a class of TIDs, are generally comprised of a multi-strand seal wire that is threaded through or around key features and a unique seal body that captures and restrains the seal wire. Seal integrity resides with unique identification of the seal and the integrity of the seal body and the seal wire. Upon inspection, the seal wire may be cut and the full length inspected. A new seal may be applied in the field as a replacement, if desired. Seal wire inspection typically requires visual and tactile examinations, which are both subjective. A need therefore exists to develop seal wire inspection technology that is easy to use in the field, is objective, provides an auditable data trail, and has low error rates. Expected benefits, if successfully implemented, are improved on-site inspection reliability and security. The work scope for this effort was restricted to integrity of seal wire used by the International Atomic Energy Agency (IAEA) and resulted in development of a wire integrity verification instrument (WIVI) laboratory prototype. Work included a performance evaluation of a laboratory-bench-top system, and design and delivery of two WIVI laboratory prototypes. The paper describes the basic physics of the eddy current measurement, a description of the WIVI laboratory prototype, and an initial evaluation performed by IAEA personnel. --- Funding was provided by the U.S. Program for Technical Assistance to IAEA Safeguards (POTAS).

  3. Seal formation in arid soil under natural and laboratory conditions

    NASA Astrophysics Data System (ADS)

    Sarah, Pariente; Sachs, Eyal

    2013-04-01

    Runoff is of considerable importance in the functioning of a desert ecosystem. The hydrological characteristics of runoff developing on arid soil under natural field conditions and those of runoff occurring in laboratory-controlled rain simulation experiments using the same type of soil were investigated. Runoff and erosion measurements were carried out in small plots (0.2-0.8 m2) on a south-facing hillslope in the northern Negev, Israel (90 mm ave. annual rainfall). Soil from the area near to the runoff plots was collected for the rain simulation experiments conducted in the laboratory. The soil was collected from 0-1 cm and 1-5 cm depths, and then placed within boxes (1.16 m long and 0.55 m wide) in the laboratory in the same order as they had been in the field. Representative surface stones were collected in the field and scattered randomly on the soil surface in the laboratory boxes. In some of the laboratory experiments soil, 5 cm in depth, was placed on a geotechnical sheet on a metal screen, while in other experiments, soil of 5 cm depth was placed on a Terzaghi filter. Rain simulator used had a rotating disk with a tilted nozzle to simulate raindrop size dispersion and kinetic energy of natural rain. The sprinkling intensity was set at a rate of 18 mm/hour. Soil crusts in the field were more stable than those created in the lab for two standard tests: Emerson - immersion test, and the 'single water drop' test. Whereas weak activity of microphytes was found in the field there was no such activity in the lab. The rain depth until runoff in the field was less than under laboratory conditions, while the sediment yield was greater in the field than in the laboratory (8.64 g/m2 versus 0.58 g/m2). The rain simulator experiments that had included a Terzaghi filter showed significantly higher final infiltration rate (7.5 mm/h versus 4.2 mm/h), shorter accumulated watering depth until stabilization of soil seal formation (100-200 mm versus 50 mm), and smaller

  4. Laboratory Testing of Aerosol for Enclosure Air Sealing

    SciTech Connect

    Harrington, Curtis; Modera, Mark

    2012-05-01

    This report presents a process for improving the air tightness of a building envelope by sealing shell leaks with an aerosol sealing technology. Both retrofit and new construction applications are possible through applying this process either in attics and crawlspaces or during rough-in stage.

  5. Laboratory Testing of Aerosol for Enclosure Air Sealing

    SciTech Connect

    Harrington, C.; Modera, M.

    2012-05-01

    Space conditioning energy use can be significantly reduced by addressing uncontrolled infiltration and exfiltration through the envelope of a building. A process for improving the air tightness of a building envelope by sealing shell leaks with an aerosol sealing technology is presented. Both retrofit and new construction applications are possible through applying this process either in attics and crawlspaces or during rough-in stage.

  6. Laboratory development and field demonstration of self-sealing/self-healing landfill liner.

    PubMed

    Shi, Caijun; Booth, Rob

    2005-01-01

    The self-sealing/self-healing (SS/SH) barrier concept is based on the principle that two or more parent materials placed in vertical or horizontal layers will react at their interfaces to form insoluble reaction products. These products constitute a seamless impermeable seal, which is resistant to the transmission of leachate and contaminants. A SS/SH liner formulation was developed in the laboratory and demonstrated at the Sudokwon landfill site in South Korea. Laboratory testing results indicated that a seal with a hydraulic conductivity less than 10(-9) m/s formed after two to four weeks of curing at room temperature, and the seal healed itself after it was fractured. The use of the soil from the Sudokwon landfill site instead of sand as the matrix of the parent materials in the SS/SH liner retarded the sealing and healing of the seal, but did not show an obvious effect on the overall sealing and healing capacity of the seal at early stages. The construction and installation of the field demonstration SS/SH liner were carried out in the same way as for a soil cement liner. The quality of the liner was ensured by the enforcement of quality analysis/quality control procedures during installation. A single sealed ring infiltration test was performed on the field demonstration liner 36 days after the installation was completed. The measurement of water infiltration rate indicated that the liner healed after it was fractured. However, the long-term sealing and healing capacity needs to be further investigated.

  7. Field and laboratory testing of seal materials proposed for the Waste Isolation Pilot Plant

    SciTech Connect

    Knowles, M.K.; Howard, C.L.

    1996-02-05

    The Small Scale Seal Performance Tests (SSSPT) were a series of in situ tests designed to evaluate the feasibility of various materials for sealing purposes. Testing was initiated in 1985 and concluded in 1995. Materials selected for the SSSPT included salt-saturated concrete, a 50%/50% mixture of crushed salt and bentonite, bentonite, and crushed salt. This paper presents a summary of the SSSPT field program, results of the in situ testing, and a discussion of post-testing laboratory studies of salt-saturated concrete. Results of the SSSPT support the use of salt-saturated concrete, compacted bentonite clay, and compacted crushed salt as sealing materials for the WIPP.

  8. Python fiber optic seal

    SciTech Connect

    Ystesund, K.; Bartberger, J.; Brusseau, C.; Fleming, P.; Insch, K.; Tolk, K.

    1993-08-01

    Sandia National Laboratories has developed a high security fiber optic seal that incorporates tamper resistance features that are not available in commercial fiber optic seals. The Python Seal is a passive fiber optic loop seal designed to give indication of unauthorized entry. The seal includes a fingerprint feature that provides seal identity information in addition to the unique fiber optic pattern created when the seal is installed. The fiber optic cable used for the seal loop is produced with tamper resistant features that increase the difficulty of attacking that component of a seal. A Seal Reader has been developed that will record the seal signature and the fingerprint feature of the seal. A Correlator software program then compares seal images to establish a match or mismatch. SNL is also developing a Polaroid reader to permit hard copies of the seal patterns to be obtained directly from the seal.

  9. Boeing F3B-1

    NASA Technical Reports Server (NTRS)

    1930-01-01

    Boeing F3B-1: While most Boeing F3B-1s served aboard the U. S. Navy aircraft carriers Lexington and Saratoga, this example flew in NACA hands at the Langley Memorial Aeronautical Laboratory in the late 1920's. Also known as the Boeing Model 77, the aircraft was powered by a Pratt & Whitney Wasp radial engine.

  10. Impaired immune function in seals and laboratory rats exposed to dioxin-like compounds from Baltic herring

    SciTech Connect

    Ross, P.S. |; Swart, R.L. de |; Timmerman, H.H.; Loveren, H. van; Osterhaus, A.D.M.E. ||

    1995-12-31

    Complex mixtures of lipophilic contaminants have been shown to affect certain top predators in the aquatic food chain, including seals. A recent demonstration that harbor seals (Phoca vitulina) fed Baltic Sea herring displayed impaired natural killer cell activity and T-lymphocyte function represented the first demonstration of immunotoxicity induced by ambient levels of contaminants in the environment. While these animals had a lower ability to respond to immunizations with inactivated vaccines, specific antibody responses, and in vitro antigen-specific lymphoproliferative responses, obvious constraints limited the ability to extend these results with host resistance tests or an evaluation of thymus and other lymphoid organs. The authors therefore set up a parallel study by exposing pregnant laboratory rats to the same Baltic herring contaminant mixture as received the seals. They then examined immune function parameters and host resistance to virus infection. As in the seals, rat pups of the Baltic group had impaired T-lymphocyte function. In addition, thymus cells and/or their precursors appeared to be targeted, as their numbers and function were reduced in the rats. Following challenge with rat cytomegalovirus in a host resistance study, rat pups in the Baltic group had impaired natural killer cell responses to the virus infection, and lower specific CD8 + (cytotoxic T-lymphocyte) responses following in vitro stimulation. By extrapolation, these results suggest that the impaired immune responses observed in the Baltic group of seals may lead to a less effective defense against virus infections in marine mammals inhabiting polluted coastal waters. Toxicological profiles and results of both the captive seal and laboratory rat experiments tend to implicate the 2,3,7,8-TCDD-like PCB, dioxin and furan congeners in the immunosuppression, and point to a major role for the PCBs.

  11. Ceramic Seal.

    SciTech Connect

    Smartt, Heidi A.; Romero, Juan A.; Custer, Joyce Olsen; Hymel, Ross W.; Krementz, Dan; Gobin, Derek; Harpring, Larry; Martinez-Rodriguez, Michael; Varble, Don; DiMaio, Jeff; Hudson, Stephen

    2016-11-01

    Containment/Surveillance (C/S) measures are critical to any verification regime in order to maintain Continuity of Knowledge (CoK). The Ceramic Seal project is research into the next generation technologies to advance C/S, in particular improving security and efficiency. The Ceramic Seal is a small form factor loop seal with improved tamper-indication including a frangible seal body, tamper planes, external coatings, and electronic monitoring of the seal body integrity. It improves efficiency through a self-securing wire and in-situ verification with a handheld reader. Sandia National Laboratories (SNL) and Savannah River National Laboratory (SRNL), under sponsorship from the U.S. National Nuclear Security Administration (NNSA) Office of Defense Nuclear Nonproliferation Research and Development (DNN R&D), have previously designed and have now fabricated and tested Ceramic Seals. Tests have occurred at both SNL and SRNL, with different types of tests occurring at each facility. This interim report will describe the Ceramic Seal prototype, the design and development of a handheld standalone reader and an interface to a data acquisition system, fabrication of the seals, and results of initial testing.

  12. Seals and Sealing Techniques

    NASA Technical Reports Server (NTRS)

    1972-01-01

    Developments by the aerospace industry in seals and sealing techniques are announced for possible use in other areas. The announcements presented are grouped as: sealing techniques for cryogenic fluids, high pressure applications, and modification for improved performance.

  13. Development of a high temperature ceramic-to-metal seal for Air Force Weapons Laboratory Laser

    NASA Astrophysics Data System (ADS)

    Honnell, R. E.; Stoddard, S. D.

    1987-03-01

    Procedures were developed for fabricating vacuum tight metal-to-ceramic ring seals between Inconel 625 and MgO-3 wt % Y2O3 tubes metallized with a calcia-alumina-silica glass (CaO-29 wt % Al2O3-35 wt % SiO2) containing 50 vol % molybdenum filler. Palniro No. 1 (Au-25 wt % Pd-25 wt % Ni) was found to be the most reliable braze for joining Inconel to metallized MgO-3 wt % Y2O3 bodies. The reliabilities of the processing procedures and the material systems were demonstrated. A prototype electrical feedthrough was fabricated for 1173 K operation in air or vacuum.

  14. Titanium sealing glasses and seals formed therefrom

    DOEpatents

    Brow, R.K.; McCollister, H.L.; Phifer, C.C.; Day, D.E.

    1997-12-02

    Alkaline-earth lanthanoborate sealing-glass compositions containing CaO, La{sub 2}O{sub 3}, B{sub 2}O{sub 3}, TiO{sub 2} and Al{sub 2}O{sub 3} in various combinations of mole-% are provided. These sealing-glass compositions are useful for forming hermetic glass-to-metal seals with titanium and titanium alloys that have a high aqueous durability for component or device applications requiring exposure to moisture, water or body fluids. Particular applications of the titanium sealing-glass compositions include forming glass-to-metal seals for lithium batteries and implanted biomedical devices (e.g. batteries, pacemakers, defibrillators, pumps). 2 figs.

  15. Titanium sealing glasses and seals formed therefrom

    DOEpatents

    Brow, Richard K.; McCollister, Howard L.; Phifer, Carol C.; Day, Delbert E.

    1997-01-01

    Alkaline-earth lanthanoborate sealing-glass compositions containing CaO, La.sub.2 O.sub.3, B.sub.2 O.sub.3, TiO.sub.2 and Al.sub.2 O.sub.3 in various combinations of mole-% are provided. These sealing-glass compositions are useful for forming hermetic glass-to-metal seals with titanium and titanium alloys that have a high aqueous durability for component or device applications requiring exposure to moisture, water or body fluids. Particular applications of the titanium sealing-glass compositions include forming glass-to-metal seals for lithium batteries and implanted biomedical devices (e.g. batteries, pacemakers, defibrillators, pumps).

  16. Acoustic seal

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M. (Inventor)

    2006-01-01

    The invention relates to a sealing device having an acoustic resonator. The acoustic resonator is adapted to create acoustic waveforms to generate a sealing pressure barrier blocking fluid flow from a high pressure area to a lower pressure area. The sealing device permits noncontacting sealing operation. The sealing device may include a resonant-macrosonic-synthesis (RMS) resonator.

  17. Acoustic Seal

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M. (Inventor)

    2006-01-01

    The invention relates to a sealing device having an acoustic resonator. The acoustic resonator is adapted to create acoustic waveforms to generate a sealing pressure barrier blocking fluid flow from a high pressure area to a lower pressure area. The sealing device permits noncontacting sealing operation. The sealing device may include a resonant-macrosonic-synthesis (RMS) resonator.

  18. Fluid seals development for coal liquefaction slurry pumps. Quarterly technical progress report No. 11, 1 April 1985-30 June 1985. [Reciprocating pumps

    SciTech Connect

    Burcham, R.E.

    1985-07-22

    This quarterly progress report covers the work performed during the period 1 April 1985 to 30 June 1985 on the Fluid Seals Development for Coal Liquefaction Slurry Pumps Program. The work was sponsored by the Department of Energy, Pittsburgh Technology Center, to develop technology for hydrostatic fluid seals to be used in coal slurry centrifugal and reciprocating pumps. The scope of the program consists of the following tasks: (1) Task 1A: Survey of current practices for centrifugal and reciprocating coal slurry pump seals; (2) Task 1B: Preliminary evaluation of three alternative centrifugal and reciprocating fluid seal concepts; (3) Task 2: Detail evaluation of the best two centrifugal and reciprocating fluid seal concepts; (4) Task 3A: Design and fabrication of the selected centrifugal and reciprocating fluid seal concept, Design and fabrication of the centrifugal and reciprocating seal testers; (5) Task 3B: Laboratory seal testing of the centrifugal and reciprocating fluid seals; and (6) Task 4: Field testing of the centrifugal and reciprocating fluid seals. Tasks 1, 2, 3A, and 3B have been completed. Task 4 is in progress. 2 figs.

  19. Fluid seals development for coal liquefaction slurry pumps. Quarterly technical progress report No. 10, 1 January 1985-31 March 1985. [Reciprocating and centrifugal pumps

    SciTech Connect

    Burcham, R.E.

    1985-04-22

    This quarterly progress report covers the work performed during the period January 1, 1985 to March 31, 1985 on the Fluid Seals Development for Coal Liquefaction Slurry Pumps Program. The work was sponsored by the Department of Energy, Pittsburgh Technology Center, to develop technology for hydrostatic fluid seals to be used in coal slurry centrifugal and reciprocating pumps. The scope of the program consists of the following tasks: (1) Task 1A, survey of current practices for centrifugal and reciprocating coal slurry pump seals; (2) Task 1B, preliminary evaluation of three alternative centrifugal and reciprocating fluid seal concepts; (3) Task 2, detail evaluation of the best two centrifugal and reciprocating fluid seal concepts; (4) Task 3A, design and fabrication of the selected centrifugal and reciprocating fluid seal concept, design and fabrication of the centrifugal and reciprocating seal testers; (5) Task 3B, laboratory seal testing of the centrifugal and reciprocating fluid seals; (6) Task 4, field testing of the centrifugal and reciprocating fluid seals. Tasks 1, 2, 3A, and 3B have been completed. Task 4 is pending Department of Energy approval. 2 figs., 1 tab.

  20. Glass sealing

    SciTech Connect

    Brow, R.K.; Kovacic, L.; Chambers, R.S.

    1996-04-01

    Hernetic glass sealing technologies developed for weapons component applications can be utilized for the design and manufacture of fuel cells. Design and processing of of a seal are optimized through an integrated approach based on glass composition research, finite element analysis, and sealing process definition. Glass sealing procedures are selected to accommodate the limits imposed by glass composition and predicted calculations.

  1. Coining seal

    DOEpatents

    Mancebo, Lloyd

    1976-01-01

    A bakeable high pressure-vacuum seal is provided in which an inductile sealing element having a butterfly shaped crosssection with protruding sharp edges at each of the four corners, is sandwiched between two ductile sealing elements, the sandwiched assembly then being compressed between the surfaces of the flange elements of a high pressure or high vacuum vessel to coin the ductile sealing element into the surface of the inductile sealing element as well as the surfaces of the flange elements.

  2. Sealing device

    SciTech Connect

    Garcia-Crespo, Andres Jose

    2013-12-10

    A sealing device for sealing a gap between a dovetail of a bucket assembly and a rotor wheel is disclosed. The sealing device includes a cover plate configured to cover the gap and a retention member protruding from the cover plate and configured to engage the dovetail. The sealing device provides a seal against the gap when the bucket assemply is subjected to a centrifugal force.

  3. Security seal

    DOEpatents

    Gobeli, Garth W.

    1985-01-01

    Security for a package or verifying seal in plastic material is provided by a print seal with unique thermally produced imprints in the plastic. If tampering is attempted, the material is irreparably damaged and thus detectable. The pattern of the imprints, similar to "fingerprints" are recorded as a positive identification for the seal, and corresponding recordings made to allow comparison. The integrity of the seal is proved by the comparison of imprint identification records made by laser beam projection.

  4. 28 CFR 803.3 - Use of the seal.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 2 2014-07-01 2014-07-01 false Use of the seal. 803.3 Section 803.3... SEAL § 803.3 Use of the seal. (a) Each Agency's seal is used by that Agency's staff for official Agency....3. (b) Use of the Agency seal by any person or organization outside of the Agency may be made...

  5. 28 CFR 803.3 - Use of the seal.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 28 Judicial Administration 2 2013-07-01 2013-07-01 false Use of the seal. 803.3 Section 803.3... SEAL § 803.3 Use of the seal. (a) Each Agency's seal is used by that Agency's staff for official Agency....3. (b) Use of the Agency seal by any person or organization outside of the Agency may be made...

  6. Seal assembly

    DOEpatents

    Johnson, Roger Neal; Longfritz, William David

    2001-01-01

    A seal assembly that seals a gap formed by a groove comprises a seal body, a biasing element, and a connection that connects the seal body to the biasing element to form the seal assembly. The seal assembly further comprises a concave-shaped center section and convex-shaped contact portions at each end of the seal body. The biasing element is formed from an elastic material and comprises a convex-shaped center section and concave-shaped biasing zones that are opposed to the convex-shaped contact portions. The biasing element is adapted to be compressed to change a width of the seal assembly from a first width to a second width that is smaller than the first width. In the compressed state, the seal assembly can be disposed in the groove. After release of the compressing force, the seal assembly expands. The contact portions will move toward a surface of the groove and the biasing zones will move into contact with another surface of the groove. The biasing zones will bias the contact portions of the seal body against the surface of the groove.

  7. Sealing glasses for titanium and titanium alloys

    DOEpatents

    Brow, Richard K.; Watkins, Randall D.

    1992-01-01

    Glass compositions containing CaO, Al.sub.2 O.sub.3, B.sub.2 O.sub.3, SrO and BaO of various combinations of mole % are provided. These compositions are capable of forming stable glass-to-metal seals with titanium and titanium alloys, for use in components such as seals for battery headers.

  8. Sealing glasses for titanium and titanium alloys

    DOEpatents

    Brow, R.K.; Watkins, R.D.

    1988-01-21

    Glass compositions containing CaO, Al/sub 2/O/sub 3/, B/sub 2/O/sub 3/, SrO and BaO of various combinations of mole % are provided. These compositions are capable of forming stable glass-to-metal seals with titanium and titanium alloys, for use in components such as seals for battery headers.

  9. Nozzle seal

    DOEpatents

    Groff, Russell Dennis; Vatovec, Richard John

    1978-06-11

    In an illustrative embodiment of the invention, a nuclear reactor pressure vessel, having an internal hoop from which the heated coolant emerges from the reactor core and passes through to the reactor outlet nozzles, is provided with annular sealing members operatively disposed between the outlet nozzle and the hoop and partly within a retaining annulus formed in the hoop. The sealing members are biased against the pressure vessel and the hoop and one of the sealing members is provided with a piston type pressure ring sealing member which effectively closes the path between the inlet and outlet coolants in the region about the outlet nozzle establishing a leak-proof condition. Furthermore, the flexible responsiveness of the seal assures that the seal will not structurally couple the hoop to the pressure vessel.

  10. Ferrules seals

    DOEpatents

    Smith, James L.

    1984-01-01

    A device is provided for sealing an inner tube and an outer tube without excessively deforming the tubes. The device includes two ferrules which cooperate to form a vacuum-tight seal between the inner tube and outer tube and having mating surfaces such that overtightening is not possible.

  11. Ferrules seals

    DOEpatents

    Smith, J.L.

    1984-07-10

    A device is provided for sealing an inner tube and an outer tube without excessively deforming the tubes. The device includes two ferrules which cooperate to form a vacuum-tight seal between the inner tube and outer tube and having mating surfaces such that overtightening is not possible. 3 figs.

  12. Seal arrangement

    DOEpatents

    Lundholm, Gunnar

    1987-01-01

    A seal arrangement is provided for preventing gas leakage along a reciprocating piston rod or other reciprocating member passing through a wall which separates a high pressure gas chmber and a low pressure gas chamber. Liquid lubricant is applied to the lower pressure side of a sealing gland surrounding the piston rod to prevent the escape of gas between the rod and the gland. The sealing gland is radially forced against the piston rod by action of a plurality of axially stacked O-rings influenced by an axially acting spring as well as pressure from the gas.

  13. Oil Saving Seal

    NASA Technical Reports Server (NTRS)

    1978-01-01

    Driven under difficult field conditions, the Army Jeep shown went more than 22,000 miles without an oil change in a test conducted by the U.S. Army Mobility Equipment Research and Development Command. Key to this exceptionally long oil life was a set of piston ring seals made of a new synthetic rubber formula called RC-34; the seal pictured, photographed after its arduous Army trial, shows no signs of deterioration. The seal and the RC-34 material, which may soon be available for use in the family auto, were developed by Ramsey Corporation, St. Louis, Missouri, a division of TRW Automotive Worldwide. The oil in an automobile engine must be I replaced every few thousand miles not because it wears out but because it becomes contaminated. The contamination sources are gasoline and combustion gases which blow by the piston rings to mix with the oil, reducing the oil's ability to lubricate properly. Seeking to prolong oil life by eliminating "blowby," Ramsey Corporation looked for a better way to seal piston rings and used NASA technology as a departure point. The parent company TRW, under contract to NASA's Jet Propulsion Laboratory, had developed seals and bladders from a type of material called elastomers which where designed to withstand the environmental extremes of interplanetary flight. That effort formed a knowledge base for research which culminated in Ramsey's RC-34 elastomer.

  14. Shape memory alloy seals for geothermal applications

    SciTech Connect

    Friske, Warren H.; Schwartzbart, Harry

    1982-10-08

    Rockwell International's Energy Systems Group, under contract to Brookhaven National Laboratory, has completed a 2-year program to develop a novel temperature-actuated seal concept for geothermal applications. This seal concept uses the unique properties of a shape memory alloy (Nitinol) to perform the sealing function. The several advantages of the concept are discussed in the paper. Demonstration tests of both face and shaft seals have shown that leaktight seals are feasible. Supporting materials studies have included corrosion tests in geothermal fluids, elevated temperature tensile tests, experimental electroplating and metallographic evaluations of microstructures.

  15. Refractory Glass Seals for SOFC

    SciTech Connect

    Chou, Y. S.; Stevenson, Jeffry W.

    2011-07-01

    One of the critical challenges facing planar solid oxide fuel cell (SOFC) technology is the need for reliable sealing technology. Seals must exhibit long-term stability and mechanical integrity in the high temperature SOFC environment during normal and transient operation. Several different approaches for sealing SOFC stacks are under development, including glass or glass-ceramic seals, metallic brazes, and compressive seals. Among glass seals, rigid glass-ceramics, self-healing glass, and composite glass approaches have been investigated under the SECA Core Technology Program. The U.S. Department of Energy's Pacific Northwest National Laboratory (PNNL) has developed the refractory glass approach in light of the fact that higher sealing temperatures (e.g., 930-1000 degrees C) may enhance the ultimate in-service bulk strength and electrical conductivity of contact materials, as well as the bonding strength between contact materials and adjacent SOFC components, such as interconnect coatings and electrodes. This report summarizes the thermal, chemical, mechanical, and electrical properties of the refractory sealing glass.

  16. Forming a seal between planar sealing surfaces

    SciTech Connect

    Ezekoye, L.I.; Rusnica, E.J.; Sepp, H.A. Jr.

    1987-12-29

    A method of forming a seal between the confronting planar sealing surfaces on two annular structural members which are drawn together by axially extending bolts is described comprising the steps of: forming a seal assembly by locking a toroidal, crushable seal member within a substantially flat annular spacer member against an annular shoulder formed on the inner diameter thereof with an annular resilient member seated in a radially extending groove in the inner surface of the substantially flat annular member, the flat annular member being axially thinner than the toroidal, crushable seal member; placing the sealing assembly between the planar sealing surfaces and positively aligning the assembly relative to the axially extending bolts; and tightening the bolts to draw the planar sealing surfaces toward each other and into contact with the flat annular member while crushing the toroidal, crushable seal member to form a seal between the planar sealing surfaces.

  17. Metal and elastomer seal tests for accelerator applications

    SciTech Connect

    Welch, K.M.; McIntyre, G.T.; Tuozzolo, J.E.; Skelton, R.; Pate, D.J.; Gill, S.M.

    1989-01-01

    The vacuum system of the Alternating Gradient Synchrotron (AGS) at Brookhaven National Laboratory has more than a thousand metal vacuum seals. Also, numerous elastomer seals are used throughout the AGS to seal large beam component chambers. An accelerator upgrade program is being implemented to reduce the AGS operating pressure by x100 and improve the reliability of the vacuum system. This paper describes work in progress on metal and elastomer vacuum seals to help meet those two objectives. Tests are reported on the sealing properties of a variety of metal seals used on different sealing surfaces. Results are also given on reversible sorption properties of certain elastomers. 16 refs., 6 figs., 4 tabs.

  18. GAS SEAL

    DOEpatents

    Monson, H.; Hutter, E.

    1961-07-11

    A seal is described for a cover closing an opening in the top of a pressure vessel that may house a nuclear reactor. The seal comprises a U-shaped trough formed on the pressure vessel around the opening therein, a mass of metal in the trough, and an edge flange on the cover extending loosely into the trough and dipping into the metal mass. The lower portion of the metal mass is kept melted, and the upper portion, solid. The solid pontion of the metal mass prevents pressure surges in the vessel from expelling the liquid portion of the metal mass from the trough; the liquld portion, thus held in place by the solid portion, does not allow gas to go through, and so gas cannot escape through shrinkage holes in the solid portion.

  19. Ectopic DNMT3B expression delays leukemogenesis.

    PubMed

    Stanley, Robert F; Steidl, Ulrich

    2016-03-24

    In this issue of Blood, Schulze et al use a tetracycline-inducible Dnmt3b knock-in mouse model to investigate how DNMT3B-mediated DNA methylation affects leukemogenesis. Increased DNMT3B expression prolonged survival in retrovirally induced Myc-Bcl2– or MLL-AF9–driven leukemia, and acute myeloid leukemia (AML) patients with high expression of DNMT3B target genes showed inferior overall survival.

  20. Fluid pressure balanced seal

    NASA Technical Reports Server (NTRS)

    Marsh, H. W. (Inventor)

    1966-01-01

    A seal which increases in effectiveness with increasing pressure is presented. The seal's functional capability throughout both static and dynamic operation makes it particularly useful for sealing ball valve ports. Other features of the seal include the ability to seal two opposed surfaces simultaneously, tolerance of small misalignments, tolerance of wide temperature ranges, ability to maintain positive sealing contact under conditions of internal or external pressurization, and ability to conform to slight irregularities in seal or surface contours.

  1. Actively controlled shaft seals for aerospace applications

    NASA Technical Reports Server (NTRS)

    Salant, Richard F.

    1994-01-01

    This study experimentally investigates an actively controlled mechanical seal for aerospace applications. The seal of interest is a gas seal, which is considerably more compact than previous actively controlled mechanical seals that were developed for industrial use. In a mechanical seal, the radial convergence of the seal interface has a primary effect on the film thickness. Active control of the film thickness is established by controlling the radial convergence of the seal interface with piezoelectric actuator. An actively controlled mechanical seal was initially designed and evaluated using a mathematical model. Based on these results, a seal was fabricated and tested under laboratory conditions. The seal was tested with both helium and air, at rotational speeds up to 3770 rad/sec, and at sealed pressures as high as 1.48 x 10(exp 6) Pa. The seal was operated with both manual control and with a closed-loop control system that used either the leakage rate or face temperature as the feedback. The output of the controller was the voltage applied to the piezoelectric actuator. The seal operated successfully for both short term tests (less than one hour) and for longer term tests (four hours) with a closed-loop control system. The leakage rates were typically 5-15 slm (standard liters per minute), and the face temperatures were generally maintained below 100 C. When leakage rate was used as the feedback signal, the setpoint leakage rate was typically maintained within 1 slm. However, larger deviations occurred during sudden changes in sealed pressure. When face temperature was used as the feedback signal, the setpoint face temperature was generally maintained within 3 C, with larger deviations occurring when the sealed pressure changed suddenly.

  2. Construction Productivity Advancement Research (CPAR) Program. Improved Materials and Processes for Sealing and Resealing Joints in Portland Cement Concrete Pavements: Laboratory Study

    DTIC Science & Technology

    1993-05-01

    However, the Superseal 1614A Plant 1 sample did meet the FS SS-S-1514A test requirements when exposed to the longer heating periods of 3.0, 4.5, and 6.0...low of 5 min of Superseal 1614A, Plant 2. Results for all materials decreased with longer heating periods . Thus, areas sealed with these materials can

  3. DEVELOPMENT OF A CERAMIC TAMPER INDICATING SEAL: SRNL CONTRIBUTIONS

    SciTech Connect

    Krementz, D.; Brinkman, K.; Martinez-Rodriguez, M.; Mendez-Torres, A.; Weeks, G.

    2013-06-03

    Savannah River National Laboratory (SRNL) and Sandia National Laboratories (SNL) are collaborating on development of a Ceramic Seal, also sometimes designated the Intrinsically Tamper Indicating Ceramic Seal (ITICS), which is a tamper indicating seal for international safeguards applications. The Ceramic Seal is designed to be a replacement for metal loop seals that are currently used by the IAEA and other safeguards organizations. The Ceramic Seal has numerous features that enhance the security of the seal, including a frangible ceramic body, protective and tamper indicating coatings, an intrinsic unique identifier using Laser Surface Authentication, electronics incorporated into the seal that provide cryptographic seal authentication, and user-friendly seal wire capture. A second generation prototype of the seal is currently under development whose seal body is of Low Temperature Co-fired Ceramic (LTCC) construction. SRNL has developed the mechanical design of the seal in an iterative process incorporating comments from the SNL vulnerability review team. SRNL is developing fluorescent tamper indicating coatings, with recent development focusing on optimizing the durability of the coatings and working with a vendor to develop a method to apply coatings on a 3-D surface. SRNL performed a study on the effects of radiation on the electronics of the seal and possible radiation shielding techniques to minimize the effects. SRNL is also investigating implementation of Laser Surface Authentication (LSA) as a means of unique identification of each seal and the effects of the surface coatings on the LSA signature.

  4. Turbine with radial acting seal

    SciTech Connect

    Eng, Darryl S; Ebert, Todd A

    2016-11-22

    A floating brush seal in a rim cavity of a turbine in a gas turbine engine, where the floating brush seal includes a seal holder in which the floating brush seal floats, and a expandable seal that fits within two radial extending seal slots that maintains a seal with radial displacement of the floating brush seal and the seal holder.

  5. Dynamic Face Seal Arrangement

    NASA Technical Reports Server (NTRS)

    Dellacorte, Christopher (Inventor)

    1999-01-01

    A radial face seal arrangement is disclosed comprising a stationary seal ring that is spring loaded against a seal seat affixed to a rotating shaft. The radial face seal arrangement further comprises an arrangement that not only allows for preloading of the stationary seal ring relative to the seal seat, but also provides for dampening yielding a dynamic seating response for the radial face seal arrangement. The overall seal system, especially regarding the selection of the material for the stationary seal ring, is designed to operate over a wide temperature range from below ambient up to 900 C.

  6. Regenerator seal design

    DOEpatents

    Eckart, Francis H.

    1982-01-01

    A rotary regenerator disc matrix has a face seal with a cross arm and arcuate rim segments joined by prestress clamps to prestrain the arcuate rim seals so as to compensate seal rim twisting or coning and resultant disc face seal leakage as produced by operating thermal gradients across the seal.

  7. Sealing arrangement

    SciTech Connect

    Brandenstein, M.; Hans, R.; Kiener, H.

    1985-04-09

    An hydraulically operated clutch release has an annular piston hydraulically moveable in an annular chamber. A felt ring is mounted on a shoulder of the piston away from the chamber, to engage the bore surface of the chamber, and a holding ring is snapped onto the end of the shoulder to hold the felt ring on the shoulder. An extension of the holding ring forms a seal with the bore on the chamber, and defines, with the felt ring a dust catching recesS.

  8. Radiological Characterization Technical Report on Californium-252 Sealed Source Transuranic Debris Waste for the Off-Site Source Recovery Project at Los Alamos National Laboratory

    SciTech Connect

    Feldman, Alexander

    2014-04-24

    This document describes the development and approach for the radiological characterization of Cf-252 sealed sources for shipment to the Waste Isolation Pilot Plant. The report combines information on the nuclear material content of each individual source (mass or activity and date of manufacture) with information and data on the radionuclide distributions within the originating nuclear material. This approach allows for complete and accurate characterization of the waste container without the need to take additional measurements. The radionuclide uncertainties, developed from acceptable knowledge (AK) information regarding the source material, are applied to the summed activities in the drum. The AK information used in the characterization of Cf-252 sealed sources has been qualified by the peer review process, which has been reviewed and accepted by the Environmental Protection Agency.

  9. Regenerator seal

    DOEpatents

    Davis, Leonard C.; Pacala, Theodore; Sippel, George R.

    1981-01-01

    A method for manufacturing a hot side regenerator cross arm seal assembly having a thermally stablilized wear coating with a substantially flat wear surface thereon to seal between low pressure and high pressure passages to and from the hot inboard side of a rotary regenerator matrix includes the steps of forming a flat cross arm substrate member of high nickel alloy steel; fixedly securing the side edges of the substrate member to a holding fixture with a concave surface thereacross to maintain the substrate member to a slightly bent configuration on the fixture surface between the opposite ends of the substrate member to produce prestress therein; applying coating layers on the substrate member including a wear coating of plasma sprayed nickel oxide/calcium flouride material to define a wear surface of slightly concave form across the restrained substrate member between the free ends thereon; and thereafter subjecting the substrate member and the coating thereon to a heat treatment of 1600.degree. F. for sixteen hours to produce heat stabilizing growth in the coating layers on the substrate member and to produce a thermally induced growth stress in the wear surface that substantially equalizes the prestress in the substrate whereby when the cross arm is removed from the fixture surface following the heat treatment step a wear face is formed on the cross arm assembly that will be substantially flat between the ends.

  10. Repair, Evaluation, Maintenance, and Rehabilitation Research Program. Laboratory Techniques for Evaluating Effectiveness of Sealing Voids in Rubble-Mound Breakwaters and Jetties with Grouts and Concretes

    DTIC Science & Technology

    1990-03-01

    chemical sealants, and the Marshall stability test for the asphaltic concrete; and (g) placing specimens in the prototy, -_.vAronment, exposing the...test si- ens were placed at Treat Island , MA; Duck, NC; and Miami, FL. It was determined that sealing of rubble-mound coastal structures requires that...modulus of elasticity for the cementitious and chemical sealants, and the Marshall stability test for the asphaltic concrete. £. Placing specimens in the

  11. Damper Spring For Omega Seal

    NASA Technical Reports Server (NTRS)

    Maclaughlin, Scott T.; Montgomery, Stuart K.

    1993-01-01

    Damper spring reduces deflections of omega-cross-section seal, reducing probability of failure and extending life of seal. Spring is split ring with U-shaped cross section. Placed inside omega seal and inserted with seal into seal cavity. As omega seal compressed into cavity, spring and seal make contact near convolution of seal, and spring becomes compressed also. During operation, when seal dynamically loaded, spring limits deflection of seal, reducing stress on seal.

  12. Triple acting radial seal

    SciTech Connect

    Ebert, Todd A; Carella, John A

    2012-03-13

    A triple acting radial seal used as an interstage seal assembly in a gas turbine engine, where the seal assembly includes an interstage seal support extending from a stationary inner shroud of a vane ring, the interstage seal support includes a larger annular radial inward facing groove in which an outer annular floating seal assembly is secured for radial displacement, and the outer annular floating seal assembly includes a smaller annular radial inward facing groove in which an inner annular floating seal assembly is secured also for radial displacement. A compliant seal is secured to the inner annular floating seal assembly. The outer annular floating seal assembly encapsulates the inner annular floating seal assembly which is made from a very low alpha material in order to reduce thermal stress.

  13. Fundamentals of fluid sealing

    NASA Technical Reports Server (NTRS)

    Zuk, J.

    1976-01-01

    The fundamentals of fluid sealing, including seal operating regimes, are discussed and the general fluid-flow equations for fluid sealing are developed. Seal performance parameters such as leakage and power loss are presented. Included in the discussion are the effects of geometry, surface deformations, rotation, and both laminar and turbulent flows. The concept of pressure balancing is presented, as are differences between liquid and gas sealing. Mechanisms of seal surface separation, fundamental friction and wear concepts applicable to seals, seal materials, and pressure-velocity (PV) criteria are discussed.

  14. 18 CFR 3b.1 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Purpose. 3b.1 Section 3b.1 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES COLLECTION, MAINTENANCE, USE, AND DISSEMINATION OF RECORDS OF IDENTIFIABLE...

  15. 18 CFR 3b.3 - Notice requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Notice requirements. 3b.3 Section 3b.3 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT OF ENERGY GENERAL RULES COLLECTION, MAINTENANCE, USE, AND DISSEMINATION OF RECORDS OF...

  16. 18 CFR 3b.227 - Mailing lists.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Mailing lists. 3b.227 Section 3b.227 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT... specifically authorized by law. This provision shall not be construed to require the withholding of names...

  17. 18 CFR 3b.227 - Mailing lists.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Mailing lists. 3b.227 Section 3b.227 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY COMMISSION, DEPARTMENT... specifically authorized by law. This provision shall not be construed to require the withholding of names...

  18. Rotordynamic and Leakage Characteristics of a 4-Stage Brush Seal

    DTIC Science & Technology

    1992-12-01

    AD-A266 012 WL-TR-92-2125 .AP ROTORDYNAMIC AND LEAKAGE CHARACTERISTICS OF A 4-STAGE BRUSH SEAL K. J. CONNER D. W. CHILDS TURBOMACHINERY LABORATORIES...pre-rotation, and seal spacing. Direct damping is shown to increase with running speed; otherwise, the rotordynamic coefficients are relatively...test results for the 4-stage brush seal with an 8-cavity labyrinth showed superior rotordynamics performance for the brush seal; viz., larger values for

  19. Turbine disc sealing assembly

    DOEpatents

    Diakunchak, Ihor S.

    2013-03-05

    A disc seal assembly for use in a turbine engine. The disc seal assembly includes a plurality of outwardly extending sealing flange members that define a plurality of fluid pockets. The sealing flange members define a labyrinth flow path therebetween to limit leakage between a hot gas path and a disc cavity in the turbine engine.

  20. Mechanical seal assembly

    DOEpatents

    Kotlyar, Oleg M.

    2001-01-01

    An improved mechanical seal assembly is provided for sealing rotating shafts with respect to their shaft housings, wherein the rotating shafts are subject to substantial axial vibrations. The mechanical seal assembly generally includes a rotating sealing ring fixed to the shaft, a non-rotating sealing ring adjacent to and in close contact with the rotating sealing ring for forming an annular seal about the shaft, and a mechanical diode element that applies a biasing force to the non-rotating sealing ring by means of hemispherical joint. The alignment of the mechanical diode with respect to the sealing rings is maintained by a series of linear bearings positioned axially along a desired length of the mechanical diode. Alternative embodiments include mechanical or hydraulic amplification components for amplifying axial displacement of the non-rotating sealing ring and transferring it to the mechanical diode.

  1. Mechanical seal assembly

    DOEpatents

    Kotlyar, Oleg M.

    2002-01-01

    An improved mechanical seal assembly is provided for sealing rotating shafts with respect to their shaft housings, wherein the rotating shafts are subject to substantial axial vibrations. The mechanical seal assembly generally includes a rotating sealing ring fixed to the shaft, a non-rotating sealing ring adjacent to and in close contact with the rotating sealing ring for forming an annular seal about the shaft, and a mechanical diode element that applies a biasing force to the non-rotating sealing ring by means of hemispherical joint. The alignment of the mechanical diode with respect to the sealing rings is maintained by a series of linear bearings positioned axially along a desired length of the mechanical diode. Alternative embodiments include mechanical or hydraulic amplification components for amplifying axial displacement of the non-rotating sealing ring and transfering it to the mechanical diode.

  2. Turbine blade platform seal

    SciTech Connect

    Zagar, Thomas W.; Schiavo, Anthony L.

    2001-01-01

    A rotating blade group 90 for a turbo-machine having an improved device for sealing the gap 110 between the edges 112,114 of adjacent blade platforms 96,104. The gap 110 between adjacent blades 92,100 is sealed by a seal pin 20 its central portion 110 and by a seal plate 58,60 at each of the front 54 and rear 56 portions. The seal plates 58,60 are inserted into corresponding grooves 62,64 formed in the adjacent edges 112,114 of adjoining blades 92,100 and held in place by end plates 40,42. The end of the seal plates 58,60 may be chamfered 78,80 to improve the seal against the end plate 40,42. The seal pin 20 provides the required damping between the blades 92,100 and the seal plates 58,60 provide improved sealing effectiveness.

  3. Seal design alternatives study

    SciTech Connect

    Van Sambeek, L.L.

    1993-06-01

    This report presents the results from a study of various sealing alternatives for the WIPP sealing system. Overall, the sealing system has the purpose of reducing to the extent possible the potential for fluids (either gas or liquid) from entering or leaving the repository. The sealing system is divided into three subsystems: drift and panel seals within the repository horizon, shaft seals in each of the four shafts, and borehole seals. Alternatives to the baseline configuration for the WIPP seal system design included evaluating different geometries and schedules for seal component installations and the use of different materials for seal components. Order-of-magnitude costs for the various alternatives were prepared as part of the study. Firm recommendations are not presented, but the advantages and disadvantages of the alternatives are discussed. Technical information deficiencies are identified and studies are outlined which can provide required information.

  4. Actively Controlled Shaft Seals for Aerospace Applications

    NASA Technical Reports Server (NTRS)

    Salant, Richard F.; Wolff, Paul

    1995-01-01

    This study experimentally investigates an actively controlled mechanical seal for aerospace applications. The seal of interest is a gas seal, which is considerably more compact than previous actively controlled mechanical seals that were developed for industrial use. In a mechanical seal, the radial convergence of the seal interface has a primary effect on the film thickness. Active control of the film thickness is established by controlling the radial convergence of the seal interface with a piezoelectric actuator. An actively controlled mechanical seal was initially designed and evaluated using a mathematical model. Based on these results, a seal was fabricated and tested under laboratory conditions. The seal was tested with both helium and air, at rotational speeds up to 3770 rad/sec, and at sealed pressures as high as 1.48 x 10(exp 6) Pa. The seal was operated with both manual control and with a closed-loop control system that used either the leakage rate or face temperature as the feedback. The output of the controller was the voltage applied to the piezoelectric actuator. The seal operated successfully for both short term tests (less than one hour) and for longer term tests (four hours) with a closed-loop control system. The leakage rates were typically 5-15 slm (standard liters per minute), and the face temperatures were generally maintained below 100C. When leakage rate was used as the feedback signal, the setpoint leakage rate was typically maintained within 1 slm. However, larger deviations occurred during sudden changes in sealed pressure. When face temperature was used as the feedback signal, the setpoint face temperature was generally maintained within 3 C, with larger deviations occurring when the sealed pressure changes suddenly. the experimental results were compared to the predictions from the mathematical model. The model was successful in predicting the trends in leakage rate that occurred as the balance ratio and sealed pressure changed

  5. Actively controlled shaft seals for aerospace applications

    NASA Astrophysics Data System (ADS)

    Salant, Richard F.

    1995-07-01

    This study experimentally investigates an actively controlled mechanical seal for aerospace applications. The seal of interest is a gas seal, which is considerably more compact than previous actively controlled mechanical seals that were developed for industrial use. In a mechanical seal, the radial convergence of the seal interface has a primary effect on the film thickness. Active control of the film thickness is established by controlling the radial convergence of the seal interface with a piezoelectric actuator. An actively controlled mechanical seal was initially designed and evaluated using a mathematical model. Based on these results, a seal was fabricated and tested under laboratory conditions. The seal was tested with both helium and air, at rotational speeds up to 3770 rad/sec, and at sealed pressures as high as 1.48 x 10(exp 6) Pa. The seal was operated with both manual control and with a closed-loop control system that used either the leakage rate or face temperature as the feedback. The output of the controller was the voltage applied to the piezoelectric actuator. The seal operated successfully for both short term tests (less than one hour) and for longer term tests (four hours) with a closed-loop control system. The leakage rates were typically 5-15 slm (standard liters per minute), and the face temperatures were generally maintained below 100C. When leakage rate was used as the feedback signal, the setpoint leakage rate was typically maintained within 1 slm. However, larger deviations occurred during sudden changes in sealed pressure. When face temperature was used as the feedback signal, the setpoint face temperature was generally maintained within 3 C, with larger deviations occurring when the sealed pressure changes suddenly. the experimental results were compared to the predictions from the mathematical model. The model was successful in predicting the trends in leakage rate that occurred as the balance ratio and sealed pressure changed

  6. Radioactive material package seal tests

    SciTech Connect

    Madsen, M.M.; Humphreys, D.L.; Edwards, K.R.

    1990-01-01

    General design or test performance requirements for radioactive materials (RAM) packages are specified in Title 10 of the US Code of Federal Regulations Part 71 (US Nuclear Regulatory Commission, 1983). The requirements for Type B packages provide a broad range of environments under which the system must contain the RAM without posing a threat to health or property. Seals that provide the containment system interface between the packaging body and the closure must function in both high- and low-temperature environments under dynamic and static conditions. A seal technology program, jointly funded by the US Department of Energy Office of Environmental Restoration and Waste Management (EM) and the Office of Civilian Radioactive Waste Management (OCRWM), was initiated at Sandia National Laboratories. Experiments were performed in this program to characterize the behavior of several static seal materials at low temperatures. Helium leak tests on face seals were used to compare the materials. Materials tested include butyl, neoprene, ethylene propylene, fluorosilicone, silicone, Eypel, Kalrez, Teflon, fluorocarbon, and Teflon/silicone composites. Because most elastomer O-ring applications are for hydraulic systems, manufacturer low-temperature ratings are based on methods that simulate this use. The seal materials tested in this program with a fixture similar to a RAM cask closure, with the exception of silicone S613-60, are not leak tight (1.0 {times} 10{sup {minus}7} std cm{sup 3}/s) at manufacturer low-temperature ratings. 8 refs., 3 figs., 1 tab.

  7. Rotary shaft sealing assembly

    DOEpatents

    Dietle, Lannie L; Schroeder, John E; Kalsi, Manmohan S; Alvarez, Patricio D

    2013-08-13

    A rotary shaft sealing assembly in which a first fluid is partitioned from a second fluid in a housing assembly having a rotary shaft located at least partially within. In one embodiment a lip seal is lubricated and flushed with a pressure-generating seal ring preferably having an angled diverting feature. The pressure-generating seal ring and a hydrodynamic seal may be used to define a lubricant-filled region with each of the seals having hydrodynamic inlets facing the lubricant-filled region. Another aspect of the sealing assembly is having a seal to contain pressurized lubricant while withstanding high rotary speeds. Another rotary shaft sealing assembly embodiment includes a lubricant supply providing a lubricant at an elevated pressure to a region between a lip seal and a hydrodynamic seal with a flow control regulating the flow of lubricant past the lip seal. The hydrodynamic seal may include an energizer element having a modulus of elasticity greater than the modulus of elasticity of a sealing lip of the hydrodynamic seal.

  8. Rotary shaft sealing assembly

    DOEpatents

    Dietle, Lannie L.; Schroeder, John E.; Kalsi, Manmohan S.; Alvarez, Patricio D.

    2010-09-21

    A rotary shaft sealing assembly in which a first fluid is partitioned from a second fluid in a housing assembly having a rotary shaft located at least partially within. In one embodiment a lip seal is lubricated and flushed with a pressure-generating seal ring preferably having an angled diverting feature. The pressure-generating seal ring and a hydrodynamic seal may be used to define a lubricant-filled region with each of the seals having hydrodynamic inlets facing the lubricant-filled region. Another aspect of the sealing assembly is having a seal to contain pressurized lubricant while withstanding high rotary speeds. Another rotary shaft sealing assembly embodiment includes a lubricant supply providing a lubricant at an elevated pressure to a region between a lip seal and a hydrodynamic seal with a flow control regulating the flow of lubricant past the lip seal. The hydrodynamic seal may include an energizer element having a modulus of elasticity greater than the modulus of elasticity of a sealing lip of the hydrodynamic seal.

  9. The modified Cobra Seal

    SciTech Connect

    Ystesund, K.J.; Drayer, D.D.

    1990-01-01

    The Cobra Seal was developed in response to the International Atomic Energy Agency's request for an in situ verifiable seal. The Type E metal cap seal, still widely used by the IAEA, must be removed and returned to Agency headquarters for verification. The Cobra Seal allows an inspector to verify seal identity and integrity on site, without removing the seal. The seal consists of a loop of multi-strand fiber optic cable, which can be routed around or through the object to be sealed, and a seal body that secures the ends of the fiber optic cable. A cutting blade in the seal body randomly cuts a portion of the optical fibers in the cable. After the seal assembly is completed, a reference image is recorded of the unique pattern of light spots produced when the seal face is illuminated. Subsequent photographs of the seal pattern are compared to the original to establish the seal identity and integrity. This paper reviews the improvements and the technology of the cobra seal system. 3 refs., 5 figs.

  10. High temperature NASP engine seal development

    NASA Astrophysics Data System (ADS)

    This video details research being conducted at the Lewis Research Center on high temperature engine seal design for the National Aerospace Plane. To maximize the speed, the jets on the NASP extract oxygen from the air rather than carry large liquid fuel tanks; this creates temperatures within the jet of over 5000 F. To prevent these potentially explosive gases from escaping, researchers are developing new technologies for use in the engine seals. Two examples explained are the ceramic wafer seal and the braided ceramic rope seal. Computer simulations and laboratory footage are used to illustrate the workings of these seals. Benefits for other aerospace and industrial applications, as well as for the space shuttle, are explored.

  11. Nuclear reactor sealing system

    DOEpatents

    McEdwards, James A.

    1983-01-01

    A liquid metal-cooled nuclear reactor sealing system. The nuclear reactor includes a vessel sealed at its upper end by a closure head. The closure head comprises at least two components, one of which is rotatable; and the two components define an annulus therebetween. The sealing system includes at least a first and second inflatable seal disposed in series in an upper portion of the annulus. The system further includes a dip seal extending into a body of insulation located adjacent a bottom portion of the closure head. The dip seal comprises a trough formed by a lower portion of one of the components, and a seal blade pendently supported from the other component and extending downwardly into the trough. A body of liquid metal is contained in the trough which submerges a portion of the seal blade. The seal blade is provided with at least one aperture located above the body of liquid metal for providing fluid communication between the annulus intermediate the dip seal and the inflatable seals, and a body of cover gas located inside the vessel. There also is provided means for introducing a purge gas into the annulus intermediate the inflatable seals and the seal blade. The purge gas is introduced in an amount sufficient to substantially reduce diffusion of radioactive cover gas or sodium vapor up to the inflatable seals. The purge gas mixes with the cover gas in the reactor vessel where it can be withdrawn from the vessel for treatment and recycle to the vessel.

  12. Compliant Foil Seal Investigations

    NASA Technical Reports Server (NTRS)

    Proctor, Margaret; Delgado, Irebert

    2004-01-01

    Room temperature testing of an 8.5 inch diameter foil seal was conducted in the High Speed, High Temperature Turbine Seal Test Rig at the NASA Glenn Research Center. The seal was operated at speeds up to 30,000 rpm and pressure differentials up to 75 psid. Seal leakage and power loss data will be presented and compared to brush seal performance. The failure of the seal and rotor coating at 30,000 rpm and 15 psid will be presented and future development needs discussed.

  13. Inboard seal mounting

    DOEpatents

    Hayes, John R.

    1983-01-01

    A regenerator assembly for a gas turbine engine has a hot side seal assembly formed in part by a cast metal engine block having a seal recess formed therein that is configured to supportingly receive ceramic support blocks including an inboard face thereon having a regenerator seal face bonded thereto. A pressurized leaf seal is interposed between the ceramic support block and the cast metal engine block to bias the seal wear face into sealing engagement with a hot side surface of a rotary regenerator matrix.

  14. Advanced Duct Sealing Testing

    SciTech Connect

    Sherman, Max H.; Walker, Iain S.

    2003-08-01

    Duct leakage has been identified as a major source of energy loss in residential buildings. Most duct leakage occurs at the connections to registers, plenums or branches in the duct system. At each of these connections a method of sealing the duct system is required. Typical sealing methods include tapes or mastics applied around the joints in the system. Field examinations of duct systems have typically shown that these seals tend to fail over extended periods of time. The Lawrence Berkeley National Laboratory has been testing sealant durability for several years. Typical duct tape (i.e. fabric backed tapes with natural rubber adhesives) was found to fail more rapidly than all other duct sealants. This report summarizes the results of duct sealant durability testing of five UL 181B-FX listed duct tapes (three cloth tapes, a foil tape and an Oriented Polypropylene (OPP) tape). One of the cloth tapes was specifically developed in collaboration with a tape manufacturer to perform better in our durability testing. The first test involved the aging of common ''core-to-collar joints'' of flexible duct to sheet metal collars, and sheet metal ''collar-to-plenum joints'' pressurized with 200 F (93 C) air. The second test consisted of baking duct tape specimens in a constant 212 F (100 C) oven following the UL 181B-FX ''Temperature Test'' requirements. Additional tests were also performed on only two tapes using sheet metal collar-to-plenum joints. Since an unsealed flexible duct joint can have a variable leakage depending on the positioning of the flexible duct core, the durability of the flexible duct joints could not be based on the 10% of unsealed leakage criteria. Nevertheless, the leakage of the sealed specimens prior to testing could be considered as a basis for a failure criteria. Visual inspection was also documented throughout the tests. The flexible duct core-to-collar joints were inspected monthly, while the sheet metal collar-to-plenum joints were inspected

  15. Sealing of the Morsleben Repository, Germany

    SciTech Connect

    Ellers, G.; Mauke, R.; Glass, R.; Preus, J; BfS; Fischle, W.; Linn, M.; Schmidt, S.; Muller-Hoeppe, N.; Schrimpf, C.

    2003-02-24

    In the context of the closure of the LILW-Repository Morsleben (ERAM) 26 drift seals must be erected. Being situated in the access drifts to the disposal areas these drift seals are of relevance to long-term repository safety. Their adequate hydraulic resistance has to be proved. The hydraulic resistance of drift seals depends on three main elements, the excavation disturbed zone (EDZ) of the drift, the sealing body and the contact zone between sealing body and the surrounding salt. To assess the hydraulic resistance of the EDZ and the sealing body a reliable data basis is already available. The data are given in this paper. An adequate data basis is not available yet to rate the contact zone, however. To overcome this problem in situ tests are planned investigating the contact zone of the ten-year-old Asse seal. Additionally, laboratory tests will be performed using core samples from the Asse seal. The test program is described in this paper.

  16. Sealed source peer review plan

    SciTech Connect

    Feldman, Alexander; Leonard, Lee; Burns, Ron

    2009-01-01

    Sealed sources are known quantities of radioactive materials that have been encapsulated in quantities that produce known radiation fields. Sealed sources have multiple uses ranging from instrument calibration sources to sources that produce radiation fields for experimental applications. The Off-Site Source Recovery (OSR) Project at Los Alamos National Laboratory (LANL), created in 1999, under the direction of the Waste Management Division of the U.S. Department of Energy (DOE) Albuquerque has been assigned the responsibility to recover and manage excess and unwanted radioactive sealed sources from the public and private sector. LANL intends to ship drums containing qualified sealed sources to the Waste Isolation Pilot Plant (WIPP) for disposal. Prior to shipping, these drums must be characterized with respect to radiological content and other parameters. The U. S. Environmental Protection Agency (EPA) requires that ten radionulcides be quantified and reported for every container of waste to be disposed in the WIPP. The methods traditionally approved by the EPA include non-destructive assay (NDA) in accordance with Appendix A of the Contact-Handled Transuranic Waste Acceptance Criteria for the Waste Isolation Pilot Plant (DOE, 2002) (CH WAC). However, because of the nature and pedigree of historical records for sealed sources and the technical infeasibility of performing NDA on these sources, LANL proposes to characterize the content of these waste drums using qualified existing radiological data in lieu of direct measurement. This plan describes the process and documentation requirements for the use of the peer review process to qualify existing data for sealed radiological sources in lieu of perfonning radioassay. The peer review process will be performed in accordance with criteria provided in 40 CFR {section} 194.22 which specifies the use of the NUREG 1297 guidelines. The plan defines the management approach, resources, schedule, and technical requirements

  17. Energy efficient face seal

    NASA Technical Reports Server (NTRS)

    Sehnal, J.; Sedy, J.; Etsion, I.; Zobens, A.

    1982-01-01

    Torque, face temperature, leakage, and wear of a flat face seal were compared with three coned face seals at pressures up to 2758 kPa and speeds up to 8000 rpm. Axial movement of the mating seal parts was recorded by a digital data acquisition system. The coning of the tungsten carbide primary ring ranged from .51 micro-m to 5.6 micro-m. The torque of the coned face seal balanced to 76.3% was an average 42% lower, the leakage eleven times higher, than that of the standard flat face seal. The reduction of the balance of the coned face seal to 51.3% resulted by decreasing the torque by an additional 44% and increasing leakage 12 to 230 times, depending on the seal shaft speed. No measurable wear was observed on the face of the coned seals.

  18. Sealed container sampling device

    NASA Technical Reports Server (NTRS)

    Hennigan, T. J.

    1969-01-01

    Sampling device, by means of a tapered needle, pierces a sealed container while maintaining the seal and either evacuates or pressurizes the container. This device has many applications in the chemical, preservative and battery-manufacturing industries.

  19. Magnetically Actuated Seal

    NASA Technical Reports Server (NTRS)

    Pinera, Alex

    2013-01-01

    This invention is a magnetically actuated seal in which either a single electromagnet, or multiple electromagnets, are used to control the seal's position. This system can either be an open/ close type of system or an actively controlled system.

  20. High expansion, lithium corrosion resistant sealing glasses

    DOEpatents

    Brow, Richard K.; Watkins, Randall D.

    1991-01-01

    Glass compositions containing CaO, Al.sub.2 O.sub.3, B.sub.2 O.sub.3, SrO and BaO in various combinations of mole % are provided. These compositions are capable of forming stable glass-to-metal seals with pin materials of 446 Stainless Steel and Alloy-52 rather than molybdenum, for use in harsh chemical environments, specifically in lithium batteries.

  1. High expansion, lithium corrosion resistant sealing glasses

    DOEpatents

    Brow, R.K.; Watkins, R.D.

    1991-06-04

    Glass compositions containing CaO, Al[sub 2]O[sub 3], B[sub 2]O[sub 3], SrO and BaO in various combinations of mole % are provided. These compositions are capable of forming stable glass-to-metal seals with pin materials of 446 Stainless Steel and Alloy-52 rather than molybdenum, for use in harsh chemical environments, specifically in lithium batteries.

  2. The Role of Semaphorin 3B (SEMA3B) in the Pathogenesis of Breast Cancer

    DTIC Science & Technology

    2006-04-01

    of a plasmid encoding SEMA3B into H1299 non-small cell lung cancer (NSCLC) cells lead to induction of apoptosis and a dramatic decrease in colony...treated with Cos7 media after transfection with SEMA3B, or control vector (Figure 1). It is important to point out that the lung cancer line H1299 is...SEMA3B effect. In conclusion we have found that most cells lines will respond to SEMA3B growth inhibition. 0 50 100 150 H1299 H2009 H44 HCC1806

  3. Tamper-indicating seal

    DOEpatents

    Fiarman, Sidney; Degen, Michael F.; Peters, Henry F.

    1985-01-01

    There is disclosed a tamper-indicating seal that permits in the field inspection and detection of tampering. Said seal comprises a shrinkable tube having a visible pattern of markings which is shrunk over the item to be sealed, and a second transparent tube, having a second visible marking pattern, which is shrunk over the item and the first tube. The relationship between the first and second set of markings produces a pattern so that the seal may not be removed without detection.

  4. Security seal. [Patent application

    DOEpatents

    Gobeli, G.W.

    1981-11-17

    Security for a package or verifying seal in plastic material is provided by a print seal with unique thermally produced imprints in the plastic. If tampering is attempted, the material is irreparably damaged and thus detectable. The pattern of the imprints, similar to fingerprints are recorded as a positive identification for the seal, and corresponding recordings made to allow comparison. The integrity of the seal is proved by the comparison of imprint identification records made by laser beam projection.

  5. Turbomachine Interface Sealing

    NASA Technical Reports Server (NTRS)

    Hendricks, Robert C.; Chupp, Raymond E.; Lattime, Scott B.; Steinetz, Bruce M.

    2005-01-01

    Sealing interfaces and coatings, like lubricants, are sacrificial, giving up their integrity for the benefit of the component. Clearance control is a major issue in power systems turbomachine design and operational life. Sealing becomes the most cost-effective way to enhance system performance. Coatings, films, and combined use of both metals and ceramics play a major role in maintaining interface clearances in turbomachine sealing and component life. This paper focuses on conventional and innovative materials and design practices for sealing interfaces.

  6. Resilient Braided Rope Seal

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M. (Inventor); Kren, Lawrence A. (Inventor)

    1996-01-01

    A resilient braided rope seal for use in high temperature applications. The resilient braided rope seal includes a center core of fibers, a resilient 5 member overbraided by at least one layer of braided sheath fibers tightly packed together. The resilient member adds significant stiffness to the seal while maintaining resiliency. Furthermore, the seal permanent set and hysteresis are greatly reduced. Finally, improved load capabilities are provided.

  7. Collapsable seal member

    DOEpatents

    Sherrell, Dennis L.

    1990-01-01

    A hollow, collapsable seal member normally disposed in a natural expanded state offering fail-safe pressure sealing against a seating surface and adapted to be evacuated by a vacuum force for collapsing the seal member to disengage the same from said seating surface.

  8. Skew resisting hydrodynamic seal

    DOEpatents

    Conroy, William T.; Dietle, Lannie L.; Gobeli, Jeffrey D.; Kalsi, Manmohan S.

    2001-01-01

    A novel hydrodynamically lubricated compression type rotary seal that is suitable for lubricant retention and environmental exclusion. Particularly, the seal geometry ensures constraint of a hydrodynamic seal in a manner preventing skew-induced wear and provides adequate room within the seal gland to accommodate thermal expansion. The seal accommodates large as-manufactured variations in the coefficient of thermal expansion of the sealing material, provides a relatively stiff integral spring effect to minimize pressure-induced shuttling of the seal within the gland, and also maintains interfacial contact pressure within the dynamic sealing interface in an optimum range for efficient hydrodynamic lubrication and environment exclusion. The seal geometry also provides for complete support about the circumference of the seal to receive environmental pressure, as compared the interrupted character of seal support set forth in U.S. Pat. Nos. 5,873,576 and 6,036,192 and provides a hydrodynamic seal which is suitable for use with non-Newtonian lubricants.

  9. Seals development and evaluation

    SciTech Connect

    Waddoups, I.G.; Horton, P.R.V.

    1994-08-01

    This paper discusses Sandia`s support of DOE`s domestic seals program. Testing was conducted on several pressure sensitive seals and a few wire loop seals currently in use as well as on a few new seals. The testing on new seals concentrated on loop seals and included two fiber optic seals and a recently available wire loop seal being considered for use. Environmental, handling and vulnerability testing were conducted. The standardized testing approach used and the results of the testing are summarized. The status of evaluations for using higher security active and passive seals for domestic applications is also presented. The conclusion of the testing -of seals currently in use is that, even though there is some variability in their ability to meet all the test criterion, they are all generally acceptable by the test standards used. The motivation for evaluating higher security seals is to ascertain if seals could be used in broader domestic environment and result in improved cost-effectiveness.

  10. Hydraulic System Seal Development.

    DTIC Science & Technology

    1981-06-01

    supplying the seal. Shamban, Fluorocarbon, Parker, Powty, Hercules, Disogrin, Bal Seal Engineering, American Variseal , Tetrafluor, Conover, and Greene...characteristics, as did the Variseal , but both caused minor rod scoring. The Greene Tweed llytrel seal was not considered for further testing becduse

  11. Sealing of boreholes using natural, compatible materials: Granular salt

    SciTech Connect

    Finley, R.E.; Zeuch, D.H.; Stormont, J.C.; Daemen, J.J.K.

    1994-05-01

    Granular salt can be used to construct high performance permanent seals in boreholes which penetrate rock salt formations. These seals are described as seal systems comprised of the host rock, the seal material, and the seal rock interface. The performance of these seal systems is defined by the complex interactions between these seal system components through time. The interactions are largely driven by the creep of the host formation applying boundary stress on the seal forcing host rock permeability with time. The immediate permeability of these seals is dependent on the emplaced density. Laboratory test results suggest that careful emplacement techniques could results in immediate seal system permeability on the order of 10{sup {minus}16} m{sup 2} to 10{sup {minus}18} m{sup 2} (10{sup {minus}4} darcy to 10{sup {minus}6}). The visco-plastic behavior of the host rock coupled with the granular salts ability to ``heal`` or consolidate make granular salt an ideal sealing material for boreholes whose permanent sealing is required.

  12. Fuel cell manifold sealing system

    DOEpatents

    Grevstad, Paul E.; Johnson, Carl K.; Mientek, Anthony P.

    1980-01-01

    A manifold-to-stack seal and sealing method for fuel cell stacks. This seal system solves the problem of maintaining a low leak rate manifold seal as the fuel cell stack undergoes compressive creep. The seal system eliminates the problem of the manifold-to-stack seal sliding against the rough stack surface as the stack becomes shorter because of cell creep, which relative motion destroys the seal. The seal system described herein utilizes a polymer seal frame firmly clamped between the manifold and the stack such that the seal frame moves with the stack. Thus, as the stack creeps, the seal frame creeps with it, and there is no sliding at the rough, tough to seal, stack-to-seal frame interface. Here the sliding is on a smooth easy to seal location between the seal frame and the manifold.

  13. Facility 3A/3B, oblique view of 3B with 3A behind from ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Facility 3A/3B, oblique view of 3B with 3A behind from Facility 1456. view facing east - U.S. Naval Base, Pearl Harbor, Instrument Shop & Electrical Shop Lean-to, Avenue E, between Sixth & Seventh Streets, Pearl City, Honolulu County, HI

  14. Properties of Laboratory-Tested Specimens of Concrete from Small-Scale Seal Performance Tests at the Waste Isolation Pilot Plant.

    DTIC Science & Technology

    1987-09-01

    REPORT 1lb TIME COVERED 14 DATE OF REPORT (Year. Month, Day) 15 PAGE COUNT Final report FROM J - j 19R5TOfebj 8 September 1987 80 IS SUPPLEMENTARY...Monitor for field and laboratory activities, which occurred between July 1985 and February 1987. Mr. Donald M. Walley and Dr. Lillian D. Wakeley, SL, CTD...I-I APPENDIX J : EQUATIONS THAT MODEL THE TEST DATA ................... J - 1 2 160 0 LIST OF TABLES No. Page 1 Components and

  15. SEAL FOR ROTATING SHAFT

    DOEpatents

    Coffman, R.T.

    1957-12-10

    A seal is described for a rotatable shaft that must highly effective when the shaft is not rotating but may be less effective while the shaft is rotating. Weights distributed about a sealing disk secured to the shaft press the sealing disk against a tubular section into which the shiilt extends, and whem the shaft rotates, the centrifugal forces on the weights relieve the pressurc of the sealing disk against the tubular section. This action has the very desirible result of minimizing the wear of the rotating disk due to contact with the tubular section, while affording maximum sealing action when it is needed.

  16. Tamper-indicating seal

    DOEpatents

    Fiarman, S.; Degen, M.F.; Peters, H.F.

    1982-08-13

    There is disclosed a tamper-indicating seal that permits in the field inspection and detection of tampering. Said seal comprises a shrinkable tube having a visible pattern of markings which is shrunk over th item to be sealed, and a second transparent tube, having a second visible marking pattern, which is shrunk over the item and the first tube. The relationship between the first and second set of markings produces a pattern so that the seal may not be removed without detection. The seal is particularly applicable to UF/sub 6/ cylinder valves.

  17. Low Cost, Durable Seal

    SciTech Connect

    Roberts, George; Parsons, Jason; Friedman, Jake

    2010-12-17

    Seal durability is critical to achieving the 2010 DOE operational life goals for both stationary and transportation PEM fuel cell stacks. The seal material must be chemically and mechanically stable in an environment consisting of aggressive operating temperatures, humidified gases, and acidic membranes. The seal must also be producible at low cost. Currentlyused seal materials do not meet all these requirements. This project developed and demonstrated a high consistency hydrocarbon rubber seal material that was able to meet the DOE technical and cost targets. Significant emphasis was placed on characterization of the material and full scale molding demonstrations.

  18. Rotary kiln seal

    SciTech Connect

    Drexler, Robert L.

    1992-01-01

    A rotary seal used to prevent the escape of contaminates from a rotating kiln incinerator. The rotating seal combines a rotating disc plate which is attached to the rotating kiln shell and four sets of non-rotating carbon seal bars housed in a primary and secondary housing and which rub on the sides of the disc. A seal air system is used to create a positive pressure in a chamber between the primary and secondary seals to create a positive air flow into the contaminated gas chamber. The seal air system also employs an air inlet located between the secondary and tertiary seals to further insure that no contaminates pass the seal and enter the external environment and to provide makeup air for the air which flows into the contaminated gas chamber. The pressure exerted by the seal bars on the rotating disc is controlled by means of a preload spring. The seal is capable of operating in a thermally changing environment where the both radial expansion and axial movement of the rotating kiln do not result in the failure of the seal.

  19. Human kidney anion exchanger 1 interacts with kinesin family member 3B (KIF3B)

    SciTech Connect

    Duangtum, Natapol; Junking, Mutita; Sawasdee, Nunghathai; Cheunsuchon, Boonyarit; Limjindaporn, Thawornchai; Yenchitsomanus, Pa-thai

    2011-09-16

    Highlights: {yields} Impaired trafficking of kAE1 causes distal renal tubular acidosis (dRTA). {yields} The interaction between kAE1 and kinesin family member 3B (KIF3B) is reported. {yields} The co-localization between kAE and KIF3B was detected in human kidney tissues. {yields} A marked reduction of kAE1 on the cell membrane was observed when KIF3B was knockdown. {yields} KFI3B plays an important role in trafficking of kAE1 to the plasma membrane. -- Abstract: Impaired trafficking of human kidney anion exchanger 1 (kAE1) to the basolateral membrane of {alpha}-intercalated cells of the kidney collecting duct leads to the defect of the Cl{sup -}/HCO{sub 3}{sup -} exchange and the failure of proton (H{sup +}) secretion at the apical membrane of these cells, causing distal renal tubular acidosis (dRTA). In the sorting process, kAE1 interacts with AP-1 mu1A, a subunit of AP-1A adaptor complex. However, it is not known whether kAE1 interacts with motor proteins in its trafficking process to the plasma membrane or not. We report here that kAE1 interacts with kinesin family member 3B (KIF3B) in kidney cells and a dileucine motif at the carboxyl terminus of kAE1 contributes to this interaction. We have also demonstrated that kAE1 co-localizes with KIF3B in human kidney tissues and the suppression of endogenous KIF3B in HEK293T cells by small interfering RNA (siRNA) decreases membrane localization of kAE1 but increases its intracellular accumulation. All results suggest that KIF3B is involved in the trafficking of kAE1 to the plasma membrane of human kidney {alpha}-intercalated cells.

  20. COMPRESSION SEAL AND SEALING MATERIAL THEREFOR

    DOEpatents

    Branin, T.G.

    1962-05-29

    This patent relates to compression seal and more particularly to a seaiing material therefor. The sealing surface is a coating consisting of alternate layers of gold and of a non-gold metal having similar plastic flow properties under pressure as gold. The coating is substantially free from oxidation effects when exposed to ambient atmosphere and does not become brittle when worked, as in a valve. (AEC)

  1. Shaft seal system

    DOEpatents

    Kapich, Davorin D.

    1985-01-01

    A shaft seal system is disclosed for isolating two regions of different fluid mediums through which a rotatable shaft extends. The seal system includes a seal housing through which the shaft extends and which defines an annular land and an annular labyrinth both of which face on the shaft so that each establishes a corresponding fluid sealing annulus. A collection cavity is formed in communication with the annular sealing spaces, and fluids compatible with the fluids in each of the two regions to be isolated are introduced, respectively, into the annular sealing spaces and collected in the collection cavity from which the fluid mixture is removed and passed to a separator which separates the fluids and returns them to their respective annular sealing spaces in a recycling manner. In the illustrated embodiment, the isolated fluid mediums comprise a liquid region and a gas region. Gas is removed from the gas region and passed through a purifier and a gas pump operative to introduce the purified gas through the labyrinth sealing annulus to the collection cavity. After passing to the separator, the separated gas is passed through a dryer from which the dried gas is caused to pass through the labyrinth sealing annulus into the collection cavity independently of the purified gas so as to insure isolation of the gas region in the event of sealing gas pump malfunction.

  2. Contracting/expanding self-sealing cryogenic tube seals

    NASA Technical Reports Server (NTRS)

    Jia, Lin X. (Inventor)

    1997-01-01

    Contracting/expanding self-sealing cryogenic tube seals are disclosed which use the different properties of thermal contraction and expansion of selected dissimilar materials in accord with certain design criteria to yield self-tightening seals via sloped-surface sealing. The seals of the subject invention are reusable, simple to assemble, and adaptable to a wide variety of cryogenic applications.

  3. Experimental Toxoplasma gondii infection in grey seals (Halichoerus grypus).

    PubMed

    Gajadhar, A A; Measures, L; Forbes, L B; Kapel, C; Dubey, J P

    2004-04-01

    Laboratory-reared animals were used to assess the susceptibility of seals (Halichoerus grypus) to Toxoplasma gondii infection. Four seals were each orally inoculated with 100 or 10,000 oocysts of T. gondii (VEG strain), and another 4 seals served as negative controls. Occasionally, mild behavioral changes were observed in all inoculated seals but not in control animals. A modified agglutination test revealed the presence of antibodies to T. gondii in sera collected from inoculated seals and mice inoculated as controls. No evidence of the parasite was found on an extensive histological examination of seal tissues, and immunohistochemical staining of tissue sections from inoculated seals revealed a single tissue cyst in only 1 seal. Control mice inoculated with 10 oocysts from the same inoculum given to seals became serologically and histologically positive for T. gondii. Cats that were fed brain or muscle tissue collected from inoculated seals passed T. gondii oocysts in feces. This study demonstrates that T. gondii oocysts can establish viable infection in seals and supports the hypothesis that toxoplasmosis in marine mammals can be acquired from oocysts in surface water runoff and sewer discharge.

  4. Layered seal for turbomachinery

    DOEpatents

    Sarawate, Neelesh Nandkumar; Morgan, Victor John; Weber, David Wayne

    2015-11-20

    The present application provides seal assemblies for reducing leakages between adjacent components of turbomachinery. The seal assemblies may include outer shims, and at least a portion of the outer shims may be substantially impervious. At least one of the outer shims may be configured for sealing engagement with seal slots of the adjacent components. The seal assemblies may also include at least one of an inner shim and a filler layer positioned between the outer shims. The at least one inner shim may be substantially solid and the at least one filler layer may be relatively porous. The seal assemblies may be sufficiently flexible to account for misalignment between the adjacent components, sufficiently stiff to meet assembly requirements, and sufficiently robust to operating meet requirements associated with turbomachinery.

  5. Seals Code Development Workshop

    NASA Technical Reports Server (NTRS)

    Hendricks, Robert C. (Compiler); Liang, Anita D. (Compiler)

    1996-01-01

    Seals Workshop of 1995 industrial code (INDSEAL) release include ICYL, GCYLT, IFACE, GFACE, SPIRALG, SPIRALI, DYSEAL, and KTK. The scientific code (SCISEAL) release includes conjugate heat transfer and multidomain with rotordynamic capability. Several seals and bearings codes (e.g., HYDROFLEX, HYDROTRAN, HYDROB3D, FLOWCON1, FLOWCON2) are presented and results compared. Current computational and experimental emphasis includes multiple connected cavity flows with goals of reducing parasitic losses and gas ingestion. Labyrinth seals continue to play a significant role in sealing with face, honeycomb, and new sealing concepts under investigation for advanced engine concepts in view of strict environmental constraints. The clean sheet approach to engine design is advocated with program directions and anticipated percentage SFC reductions cited. Future activities center on engine applications with coupled seal/power/secondary flow streams.

  6. Dynamic sealing principles

    NASA Technical Reports Server (NTRS)

    Zuk, J.

    1976-01-01

    The fundamental principles governing dynamic sealing operation are discussed. Different seals are described in terms of these principles. Despite the large variety of detailed construction, there appear to be some basic principles, or combinations of basic principles, by which all seals function, these are presented and discussed. Theoretical and practical considerations in the application of these principles are discussed. Advantages, disadvantages, limitations, and application examples of various conventional and special seals are presented. Fundamental equations governing liquid and gas flows in thin film seals, which enable leakage calculations to be made, are also presented. Concept of flow functions, application of Reynolds lubrication equation, and nonlubrication equation flow, friction and wear; and seal lubrication regimes are explained.

  7. Mechanically expandable annular seal

    DOEpatents

    Gilmore, R.F.

    1983-07-19

    A mechanically expandable annular reusable seal assembly to form an annular hermetic barrier between two stationary, parallel, and planar containment surfaces is described. A rotatable ring, attached to the first surface, has ring wedges resembling the saw-tooth array of a hole saw. Matching seal wedges are slidably attached to the ring wedges and have their motion restricted to be perpendicular to the second surface. Each seal wedge has a face parallel to the second surface. An annular elastomer seal has a central annular region attached to the seal wedges' parallel faces and has its inner and outer circumferences attached to the first surface. A rotation of the ring extends the elastomer seal's central region perpendicularly towards the second surface to create the fluid tight barrier. A counter rotation removes the barrier. 6 figs.

  8. Mechanically expandable annular seal

    DOEpatents

    Gilmore, Richard F.

    1983-01-01

    A mechanically expandable annular reusable seal assembly to form an annular hermetic barrier between two stationary, parallel, and planar containment surfaces. A rotatable ring, attached to the first surface, has ring wedges resembling the saw-tooth array of a hole saw. Matching seal wedges are slidably attached to the ring wedges and have their motion restricted to be perpendicular to the second surface. Each seal wedge has a face parallel to the second surface. An annular elastomer seal has a central annular region attached to the seal wedges' parallel faces and has its inner and outer circumferences attached to the first surface. A rotation of the ring extends the elastomer seal's central region perpendicularly towards the second surface to create the fluidtight barrier. A counterrotation removes the barrier.

  9. Sodium sulfur battery seal

    DOEpatents

    Mikkor, Mati

    1981-01-01

    This disclosure is directed to an improvement in a sodium sulfur battery construction in which a seal between various battery compartments is made by a structure in which a soft metal seal member is held in a sealing position by holding structure. A pressure applying structure is used to apply pressure on the soft metal seal member when it is being held in sealing relationship to a surface of a container member of the sodium sulfur battery by the holding structure. The improvement comprises including a thin, well-adhered, soft metal layer on the surface of the container member of the sodium sulfur battery to which the soft metal seal member is to be bonded.

  10. Compliant Turbomachine Sealing

    NASA Technical Reports Server (NTRS)

    Hendricks, R. C.; Braun, M. J.; Deng, D.; Hendricks, J. A.

    2011-01-01

    Sealing interface materials and coatings are sacrificial, giving up their integrity for the benefit of the component. Seals that are compliant while still controlling leakage, dynamics, and coolant flows are sought to enhance turbomachine performance. Herein we investigate the leaf-seal configuration. While the leaf seal is classified as contacting, a ready modification using the leaf-housing arrangement in conjunction with an interface film rider (a bore seal, for example) provides for a film-riding noncontact seal. The leaf housing and leaf elements can be made from a variety of materials from plastic to ceramic. Four simplistic models are used to identify the physics essential to controlling leakage. Corroborated by CFD, these results provide design parameters for applications to within reasonable engineering certainty. Some potential improvements are proposed.

  11. Damped flexible seal

    SciTech Connect

    DuBois, Neil J.; Amaral, Antonio M.

    1992-10-27

    A damped flexible seal assembly for a torpedo isolates the tailcone thereof rom vibrational energy present in the drive shaft assembly. A pair of outside flanges, each of which include an inwardly facing groove and an O-ring constrained therein, provide a watertight seal against the outer non-rotating surface of the drive shaft assembly. An inside flange includes an outwardly-facing groove and an O-ring constrained therein, and provides a watertight seal against the inner surface of the tail cone. Two cast-in-place elastomeric seals provide a watertight seal between the flanges and further provide a damping barrier between the outside flanges and the inside flanges for damping vibrational energy present in the drive shaft assembly before the energy can reach the tailcone through the seal assembly.

  12. Inflatable traversing probe seal

    NASA Technical Reports Server (NTRS)

    Trimarchi, Paul A.

    1991-01-01

    An inflatable seal acts as a pressure-tight zipper to provide traversing capability for instrumentation rakes and probes. A specially designed probe segment with a teardrop cross-section in the vicinity of the inflatable seal minimizes leakage at the interface. The probe is able to travel through a lengthwise slot in a pressure vessel or wind tunnel section, while still maintaining pressure integrity. The design uses two commercially available inflatable seals, opposing each other, to cover the probe slot in a wind tunnel wall. Proof-of-concept tests were conducted at vessel pressures up to 30 psig, with seals inflated to 50 psig, showing no measurable leakage along the seal's length or around the probe teardrop cross-section. This seal concept can replace the existing technology of sliding face plate/O-ring systems in applications where lengthwise space is limited.

  13. Liquid Annular Seal Research

    NASA Technical Reports Server (NTRS)

    Palazzolo, Alan B.; Venkataraman, Balaji; Padavala, Sathya S.; Ryan, Steve; Vallely, Pat; Funston, Kerry

    1996-01-01

    This paper highlights the accomplishments on a joint effort between NASA - Marshall Space Flight Center and Texas A and M University to develop accurate seal analysis software for use in rocket turbopump design, design audits and trouble shooting. Results for arbitrary clearance profile, transient simulation, thermal effects solution and flexible seal wall model are presented. A new solution for eccentric seals based on cubic spline interpolation and ordinary differential equation integration is also presented.

  14. Compliant seal development

    NASA Technical Reports Server (NTRS)

    Hendricks, Robert C.

    1993-01-01

    The compliant metallic seal combines the noncontact feature of the labyrinth seal, the low leakage of a mechanical seal, and the compliant nature of the brush seal. It consists of several thin metallic elements or leaves mounted within a ring which is press fit into the housing, and in form, sort of resembles a lip seal sections wiping the shaft. A second set of overlapping cover leaves are placed on top of the shaft riding leaves which reduces leakage and provides stiffness. The leaves can be straight or angle cut. The shaft riding fingers are designed with mismatched curvature to provide lift off similar to the Rayleigh lift pads in mechanical seals with leading edge clearances nearly twice those of the trailing edge as as shown by Fleming to be optimal for gas flows in convergent seal passages. Leading edge clearances range from 300 to 500 microinches. Balance pockets beneath the leaves provide fluid film feed to the 'Rayleigh lift' surface and the proper balance ratio (mechanical seal) when combined with the static pressure and film pressure. The leaves flex in the radial direction and accommodate thermomechanical behavior as well as axial motion and angular misalignment. In the static mode, there is a net closing force on the leaves. The seals were tested to 70 psi at speeds to 16,000 rpm or surface speeds to 330 fps and temperatures from ambient to 440 F. A slow cycle through the rig critical at 10,000 rpm induced a radial vibration response of 0.004 to 0.005 inch were accommodated by the seal. Preliminary performance data are encouraging demonstrating hydrodynamic liftoff and noncontacting operation at pressure and speeds typical of gas turbine engines. The leakage performance data are significantly better than commercial labyrinth and brush seals which should be expected as this design incorporates the features of the low leakage face or mechanical seal along with the flexibility of the brush configuration.

  15. Seals Flow Code Development

    NASA Technical Reports Server (NTRS)

    1991-01-01

    In recognition of a deficiency in the current modeling capability for seals, an effort was established by NASA to develop verified computational fluid dynamic concepts, codes, and analyses for seals. The objectives were to develop advanced concepts for the design and analysis of seals, to effectively disseminate the information to potential users by way of annual workshops, and to provide experimental verification for the models and codes under a wide range of operating conditions.

  16. Grayloc seal static tests

    SciTech Connect

    Leisher, W.B.; Biffle, J.H.

    1983-02-01

    A series of evaluation tests was performed on Grayloc seals. Helium service and standard seals, size 292, were used. Measurements were made of axial force and motion, diameter, hoop and axial strain, and helium leak rate. Leak rates were in the 10/sup -6/ atm cc/s range for the helium service seals. Pretest analytical calculations agreed reasonably well with measured makeup forces and deflections.

  17. Technology Solutions Case Study: Sealed Air-Return Plenum Retrofit

    SciTech Connect

    none,

    2012-08-01

    In this project, Pacific Northwest National Laboratory researchers greatly improved indoor air quality and HVAC performance by replacing an old, leaky air handler with a new air handler with an air-sealed return plenum with filter; they also sealed the ducts, and added a fresh air intake.

  18. Repository seals requirements study

    SciTech Connect

    1997-11-03

    The Yucca Mountain Site Characterization Project, managed by the Civilian Radioactive Waste Management System (CRWMS) Management and Operating Contractor (M and O) is conducting investigations to support the Viability Assessment and the License Application for a high-level nuclear waste repository at Yucca Mountain, Nevada. The sealing subsystem is part of the Yucca Mountain Waste Isolation System. The Yucca Mountain Site Characterization Project is currently evaluating the role of the sealing subsystem (shaft, ramp and exploratory borehole seals) in achieving the overall performance objectives for the Waste Isolation System. This report documents the results of those evaluations. This report presents the results of a repository sealing requirements study. Sealing is defined as the permanent closure of the shafts, ramps, and exploratory boreholes. Sealing includes those components that would reduce potential inflows above the repository, or that would divert flow near the repository horizon to allow vertical infiltration to below the repository. Sealing of such features as emplacement drifts was not done in this study because the current capability to calculate fracture flow into the drifts is not sufficiently mature. The objective of the study is to provide water or air flow performance based requirements for shafts, ramps, and exploratory boreholes located near the repository. Recommendations, as appropriate, are provided for developing plans, seals component testing, and other studies relating to sealing.

  19. Foil Face Seal Testing

    NASA Technical Reports Server (NTRS)

    Munson, John

    2009-01-01

    In the seal literature you can find many attempts by various researchers to adapt film riding seals to the gas turbine engine. None have been successful, potential distortion of the sealing faces is the primary reason. There is a film riding device that does accommodate distortion and is in service in aircraft applications, namely the foil bearing. More specifically a foil thrust bearing. These are not intended to be seals, and they do not accommodate large axial movement between shaft & static structure. By combining the 2 a unique type of face seal has been created. It functions like a normal face seal. The foil thrust bearing replaces the normal primary sealing surface. The compliance of the foil bearing allows the foils to track distortion of the mating seal ring. The foil seal has several perceived advantages over existing hydrodynamic designs, enumerated in the chart. Materials and design methodology needed for this application already exist. Also the load capacity requirements for the foil bearing are low since it only needs to support itself and overcome friction forces at the antirotation keys.

  20. Bidirectional Brush Seals

    NASA Technical Reports Server (NTRS)

    Hendricks, Robert C.; Wilson, Jack; Wu, Tom; Flower, Ralph

    1997-01-01

    Presented is a study of the use of a set of I.D./O.D. bidirectional press seals to reduce the leakage losses in a wave rotor. Relative to the baseline configuration, data indicate the use of brush seals enhanced wave rotor efficiency from 36 to 45 percent at low leakages (small rotor endwall gap spacings) and from 15 to 33 percent at high leakages (larger endwall gap spacings). These brush seals are capable of sealing positive or negative pressure drops with respect to the axial direction. Surface tribology for these tests suggested little evidence of grooving although the bristles appeared polished.

  1. Liquid zone seal

    DOEpatents

    Klebanoff, Leonard E.

    2001-01-01

    A seal assembly that provides a means for establishing multiple pressure zones within a system. The seal assembly combines a plate extending from the inner wall of a housing or inner enclosure that intersects with and is immersed in the fluid contained in a well formed in a tray contained within the enclosure. The fluid is a low vapor pressure oil, chemically inert and oxidation resistant. The use of a fluid as the sealing component provides a seal that is self-healing and mechanically robust not subject to normal mechanical wear, breakage, and formation of cracks or pinholes and decouples external mechanical vibrations from internal structural members.

  2. High Pressure Hydraulic Seals

    DTIC Science & Technology

    1988-09-01

    looked XV2690-18D Ring good, front seal XP2714-4 Backups, (ML1841) cracked. American Variseal /Shamban 100,493 110cc leakage on P/N AS-1272 rear seal...BACKUPS SEVERELY SEAL CEC 49S1C-214-DS DETERIORATED. 38. AMERICAN VARISEAL / TESTED THROUGH 100.493 CYCLES. EXCESSIVE LEAKAGE 110 CC SHAMBAN ON REAR SEAL...California 90230 Koppers Company, Inc. Baltimore, Maryland 21203 American Variseal Corp. Broomfield, Colorado 80020 The test actuator parts were serialized

  3. Management of Disused Radioactive Sealed Sources in Egypt - 13512

    SciTech Connect

    Mohamed, Y.T.; Hasan, M.A.; Lasheen, Y.F.

    2013-07-01

    The future safe development of nuclear energy and progressive increasing use of sealed sources in medicine, research, industry and other fields in Egypt depends on the safe and secure management of disused radioactive sealed sources. In the past years have determined the necessity to formulate and apply the integrated management program for radioactive sealed sources to assure harmless and ecological rational management of disused sealed sources in Egypt. The waste management system in Egypt comprises operational and regulatory capabilities. Both of these activities are performed under legislations. The Hot Laboratories and Waste Management Center HLWMC, is considered as a centralized radioactive waste management facility in Egypt by law 7/2010. (authors)

  4. Self Sealing Magmas

    NASA Astrophysics Data System (ADS)

    von Aulock, Felix W.; Wadsworth, Fabian B.; Kennedy, Ben M.; Lavallee, Yan

    2015-04-01

    During ascent of magma, pressure decreases and bubbles form. If the volume increases more rapidly than the relaxation timescale, the magma fragments catastrophically. If a permeable network forms, the magma degasses non-violently. This process is generally assumed to be unidirectional, however, recent studies have shown how shear and compaction can drive self sealing. Here, we additionally constrain skin formation during degassing and sintering. We heated natural samples of obsidian in a dry atmosphere and monitored foaming and impermeable skin formation. We suggest a model for skin formation that is controlled by diffusional loss of water and bubble collapse at free surfaces. We heated synthetic glass beads in a hydrous atmosphere to measure the timescale of viscous sintering. The beads sinter at drastically shorter timescales as water vapour rehydrates an otherwise degassed melt, reducing viscosity and glass transition temperatures. Both processes can produce dense inhomogeneities within the timescales of magma ascent and effectively disturb permeabilities and form barriers, particularly at the margins of the conduit, where strain localisation takes place. Localised ash in failure zones (i.e. Tuffisite) then becomes associated with water vapour fluxes and alow rapid rehydration and sintering. When measuring permeabilities in laboratory and field, and when discussing shallow degassing in volcanoes, local barriers for degassing should be taken into account. Highlighting the processes that lead to the formation of such dense skins and sintered infills of cavities can help understanding the bulk permeabilities of volcanic systems.

  5. Resilient Braided Rope Seal

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M. (Inventor); Kren, Lawrence A. (Inventor)

    2000-01-01

    A resilient braided rope seal for use in high temperature applications includes a center core of fibers. a resilient canted spring member supporting the core and at least one layer of braided sheath fibers tightly packed together overlying the spring member. The seal provides both improved load bearing and resiliency. Permanent set and hysteresis are greatly reduced.

  6. Seal ring installation tool

    NASA Technical Reports Server (NTRS)

    Haselmaier, L. Haynes (Inventor)

    2004-01-01

    A seal ring tool that allows an installer to position a primary seal ring between hub ends of pipe flanges that are being assembled together. The tool includes a pivoting handle member and extension arms attached to the pivoting handle member. The ends of the arms have side indentation type longitudinal grooves angled toward one another for holding the primary seal ring in place between the hubs of respective pipes that are to be attached together. The arms of the tool can also have flat sides that can be used to abut against an optional second larger seal that is supported within a groove in one of the hub ends so that the second hub end can then be moved against the other side of the primary seal ring. Once the seal ring is positioned between the pipe hubs, the pipe hubs can be moved about the seal ring due to the flat sides of the arms of the tool. The tool eliminates the chances of damaging and contaminating seal rings being installed within pipe hubs that are being attached to one another.

  7. Pneumatic stowing seals mines

    SciTech Connect

    Brezovec, D.

    1983-11-01

    A pneumatic stowing technique has been used in the US to seal entries to abandoned mines. Limestone mixed with dry cement or bentonite is blown into the opening. Sealing can be accomplished in much less time than with traditional concrete block/clay plug methods.

  8. Seals and Scrolls.

    ERIC Educational Resources Information Center

    Macaulay, Sara Grove

    2000-01-01

    Describes an art unit in which students sculpt a signature seal out of clay and use Chinese brush painting techniques to paint a scroll. Discusses the seal and its historical use in China. Lists materials needed and explains the procedure. (CMK)

  9. Sodium sulfur battery seal

    DOEpatents

    Topouzian, Armenag

    1980-01-01

    This invention is directed to a seal for a sodium sulfur battery in which a flexible diaphragm sealing elements respectively engage opposite sides of a ceramic component of the battery which separates an anode compartment from a cathode compartment of the battery.

  10. Repository seals requirement study

    SciTech Connect

    1997-11-03

    The Yucca Mountain Site Characterization Project, managed by the Civilian Radioactive Waste Management System (CRWMS) Management and Operating Contractor (M and O) is conducting investigations to support the Viability Assessment and the License Application for a high-level nuclear waste repository at Yucca Mountain, Nevada. The sealing subsystem is part of the Yucca Mountain Waste Isolation System. The Yucca Mountain Site Characterization Project is currently evaluating the role of the sealing subsystem (shaft, ramp and exploratory borehole seals) in achieving the overall performance objectives for the Waste Isolation System. This report documents the results of those evaluations. The objective of the study is to provide water or air flow performance based requirements for shafts, ramps, and exploratory boreholes located near the repository. Recommendations, as appropriate, are provided for developing plans, seals component testing, and other studies relating to sealing.

  11. Sealing in Turbomachinery

    NASA Technical Reports Server (NTRS)

    Chupp, Raymond E.; Hendricks, Robert C.; Lattime, Scott B.; Steinetz, Bruce M.

    2006-01-01

    Clearance control is of paramount importance to turbomachinery designers and is required to meet today's aggressive power output, efficiency, and operational life goals. Excessive clearances lead to losses in cycle efficiency, flow instabilities, and hot gas ingestion into disk cavities. Insufficient clearances limit coolant flows and cause interface rubbing, overheating downstream components and damaging interfaces, thus limiting component life. Designers have put renewed attention on clearance control, as it is often the most cost effective method to enhance system performance. Advanced concepts and proper material selection continue to play important roles in maintaining interface clearances to enable the system to meet design goals. This work presents an overview of turbomachinery sealing to control clearances. Areas covered include: characteristics of gas and steam turbine sealing applications and environments, benefits of sealing, types of standard static and dynamics seals, advanced seal designs, as well as life and limitations issues.

  12. Rotary shaft seal

    DOEpatents

    Langebrake, C.O.

    1984-01-01

    The invention is a novel rotary shaft seal assembly which provides positive-contact sealing when the shaft is not rotated and which operates with its sealing surfaces separated by a film of compressed ambient gas whose width is independent of the speed of shaft rotation. In a preferred embodiment, the assembly includes a disc affixed to the shaft for rotation therewith. Axially movable, non-rotatable plates respectively supported by sealing bellows are positioned on either side of the disc to be in sealing engagement therewith. Each plate carries piezoelectric transucer elements which are electrically energized at startup to produce films of compressed ambient gas between the confronting surfaces of the plates and the disc. Following shutdown of the shaft, the transducer elements are de-energized. A control circuit responds to incipient rubbing between the plate and either disc by altering the electrical input to the transducer elements to eliminate rubbing.

  13. Rotary shaft seal

    DOEpatents

    Langebrake, Clair O.

    1984-01-01

    The invention is a novel rotary shaft seal assembly which provides positive-contact sealing when the shaft is not rotated and which operates with its sealing surfaces separated by a film of compressed ambient gas whose width is independent of the speed of shaft rotation. In a preferred embodiment, the assembly includes a disc affixed to the shaft for rotation therewith. Axially movable, non-rotatable plates respectively supported by sealing bellows are positioned on either side of the disc to be in sealing engagement therewith. Each plate carries piezoelectric transducer elements which are electrically energized at startup to produce films of compressed ambient gas between the confronting surfaces of the plates and the disc. Following shutdown of the shaft, the transducer elements are de-energized. A control circuit responds to incipient rubbing between the plate and either disc by altering the electrical input to the transducer elements to eliminate rubbing.

  14. Transmission seal development

    NASA Technical Reports Server (NTRS)

    Brien, M.

    1977-01-01

    An experimental evaluation was performed on a high-speed (72.9 m/s, 14,349 ft/min) transmission seal of the synergistic type. During testing of the seal, oil leakage occurred at positive bearing cavity pressures. Modifications were made in an attempt to eliminate the leakage but none were completely successful. Leakage appears to be the result of questionable positioning of the sealing elements resulting in inadequate shaft contact by the oil side sealing element. This condition may be related to the nonsymmetrical shape of the elastomeric retainer and to dimensional changes caused by swelling of the elastomeric retainer from exposure to the sealed fluid. Indications of a speed dependent leakage characteristic were also observed.

  15. SSME interstage seal research

    NASA Technical Reports Server (NTRS)

    Childs, D. W.

    1984-01-01

    Test results comprising direct and transverse force coefficients and leakage coefficients are reported for six seal configurations. All seals tested use the same smooth rotor and have the same constant minimum clearance. The following stator configurations were tested: (1) Smooth, (2) knurled pattern, (3) axially-grooved pattern with end seals, (4) diamond-grid roughened, (5) diamond-grid roughened with end seals, and (6) round-hole pattern. Comparison of the seals shows the Knurled-pattern stator to be the stiffest and the round-hole pattern stator to yield the largest net damping and the least leakage. The theory of reference is shown to substantially underestimate the stiffness and effective-added-mass coefficients, but do a reasonable job in predicting the net-damping-force coefficient.

  16. Analog Video Authentication and Seal Verification Equipment Development

    SciTech Connect

    Gregory Lancaster

    2012-09-01

    Under contract to the US Department of Energy in support of arms control treaty verification activities, the Savannah River National Laboratory in conjunction with the Pacific Northwest National Laboratory, the Idaho National Laboratory and Milagro Consulting, LLC developed equipment for use within a chain of custody regime. This paper discussed two specific devices, the Authentication Through the Lens (ATL) analog video authentication system and a photographic multi-seal reader. Both of these devices have been demonstrated in a field trial, and the experience gained throughout will also be discussed. Typically, cryptographic methods are used to prove the authenticity of digital images and video used in arms control chain of custody applications. However, in some applications analog cameras are used. Since cryptographic authentication methods will not work on analog video streams, a simple method of authenticating analog video was developed and tested. A photographic multi-seal reader was developed to image different types of visual unique identifiers for use in chain of custody and authentication activities. This seal reader is unique in its ability to image various types of seals including the Cobra Seal, Reflective Particle Tags, and adhesive seals. Flicker comparison is used to compare before and after images collected with the seal reader in order to detect tampering and verify the integrity of the seal.

  17. Asphalt emulsion sealing of uranium mill tailings. 1980 annual report

    SciTech Connect

    Hartley, J.N.; Koehmstedt, P.L; Esterl, D.J.; Freeman, H.D.; Buelt, J.L.; Nelson, D.A.; Elmore, M.R.

    1981-05-01

    Studies of asphalt emulsion sealants conducted by the Pacific Northwest Laboratory have demonstrated that the sealants are effective in containing radon and other potentially hazardous material within uranium tailings. The laboratory and field studies have further demonstrated that radon exhalation from uranium tailings piles can be reduced by greater than 99% to near background levels. Field tests at the tailings pile in Grand Junction, Colorado, confirmed that an 8-cm admix seal containing 22 wt% asphalt could be effectively applied with a cold-mix paver. Other techniques were successfully tested, including a soil stabilizer and a hot, rubberized asphalt seal that was applied with a distributor truck. After the seals were applied and compacted, overburden was applied over the seal to protect the seal from ultraviolet degradation.

  18. HSCT Anticipated Seal Needs Turbomachinery Seals Combustor Seals

    NASA Technical Reports Server (NTRS)

    Henry, John

    2006-01-01

    The High Speed Civil Transport (HSCT) engine concept is a large mixed flow turbofan similar in construction to current military fighter engines. The mission, however, is quite different. The engine will operate for long periods of time at very high Mach numbers and high altitudes. The engine is required to have very low emissions and noise levels to be acceptable in commercial service. Current thrust levels are in the 55000 lb range. At the current supercruise speed requirement of Mach 2.4, the engine inlet temperature will be at least 380 F. This is the lowest cycle temperature expected anywhere in the propulsion system.Seals will be exposed to operate at this temperature and higher for thousands of hours without failure. Durability, cost, and weight will all be very important in determining the type of seals selected for a successful HSCT engine.

  19. Seals Flow Code Development 1993

    NASA Technical Reports Server (NTRS)

    Liang, Anita D. (Compiler); Hendricks, Robert C. (Compiler)

    1994-01-01

    Seals Workshop of 1993 code releases include SPIRALI for spiral grooved cylindrical and face seal configurations; IFACE for face seals with pockets, steps, tapers, turbulence, and cavitation; GFACE for gas face seals with 'lift pad' configurations; and SCISEAL, a CFD code for research and design of seals of cylindrical configuration. GUI (graphical user interface) and code usage was discussed with hands on usage of the codes, discussions, comparisons, and industry feedback. Other highlights for the Seals Workshop-93 include environmental and customer driven seal requirements; 'what's coming'; and brush seal developments including flow visualization, numerical analysis, bench testing, T-700 engine testing, tribological pairing and ceramic configurations, and cryogenic and hot gas facility brush seal results. Also discussed are seals for hypersonic engines and dynamic results for spiral groove and smooth annular seals.

  20. Reactor cavity seal ring

    SciTech Connect

    Hankinson, M.F.

    1986-04-22

    A hydrostatic seal is described for sealing an annular gap between two flat substantially horizontal coplanar surfaces comprising, in combination: a generally flat annular plate of a width sufficient to span a gap between two surfaces: compressible annular sealing means disposed on the bottom surface of the flat annular plate for sealingly engaging the two flat surfaces in response to a downward force exerted on the plate; and fastening means, distributed along the center line of the plate, for releasably fastening the plate in a position to span the gap to be sealed and exert a downward force on the plate, each fastening means including a pair of elongated members of a size to fit into the gap to be sealed, means for mounting the members on the bottom surface of the plate so that at least a portion of each member is radially moveable in a direction toward a respective one of the vertical side surfaces defining the gap to be sealed to engage same and so that the plate is moveable relative to the members in a downward direction in response to hydrostatic pressure applied to the upper surface of the plate when the members are engaging the vertical side surfaces of an annular gap, and an actuating means, mounted on the plate for movement therewith in response to hydrostatic pressure, for radially moving the members, the actuating means extending through a bore in the plate to the upper surface of the plate.

  1. A novel DNMT3B subfamily, DeltaDNMT3B, is the predominant form of DNMT3B in non-small cell lung cancer.

    PubMed

    Wang, Luo; Wang, Jie; Sun, Shiyong; Rodriguez, Marivonne; Yue, Ping; Jang, Se Jin; Mao, Li

    2006-07-01

    De novo promoter DNA methylation represses gene transcription and is a common mechanism to inactivate tumor suppressor genes in tumorigenesis. DNMT3B plays an important role in de novo DNA methylation. We report here the identification of a novel DNMT3B subfamily, termed DeltaDNMT3B, whose expression is initiated through a promoter located at intron 4 and exon 5 of the DNMT3B gene. At least 7 transcriptional variants of DeltaDNMT3B have been observed as the result of alternative pre-mRNA splicing. Predicted proteins derived from these variants suggest that 4 of the variants share a conservative enzymatic domain but contain a variable PWWP motif, a putative DNA binding structure, whereas 3 of the variants lack the enzymatic domain due to predicted premature translational termination. In non-small cell lung cancer (NSCLC) cell lines, DeltaDNMT3B variants are frequently expressed and are the predominant forms of DNMT3B. Similarly, DeltaDNMT3B variants are frequently expressed in primary NSCLC but are not detectable or are expressed at low levels in corresponding normal lung tissue. Our results indicate that DeltaDNMT3B is the major expression form of DNMT3B in NSCLC and may play an important role in the development of aberrant promoter methylation during lung tumorigenesis.

  2. Sealing device for providing a seal in a turbomachine

    DOEpatents

    Lacy, Benjamin Paul; Kottilingam, Srikanth Chandrudu; Porter, Christopher Donald; Schick, David Edward; Weber, David Wayne

    2016-08-16

    Sealing device for providing seals between adjacent components, and turbomachines utilizing such sealing devices, are provided. A sealing device includes a seal plate insertable between the adjacent components, the seal plate comprising a first face and an opposing second face. The sealing device further includes a plurality of pins extending from one of the first face or the second face, the plurality of pins configured to space the one of the first face or the second face from contact surfaces of the adjacent components.

  3. Multilayer compressive seal for sealing in high temperature devices

    DOEpatents

    Chou, Yeong-Shyung; Stevenson, Jeffry W.

    2007-08-21

    A mica based compressive seal has been developed exhibiting superior thermal cycle stability when compared to other compressive seals known in the art. The seal is composed of compliant glass or metal interlayers and a sealing (gasket) member layer composed of mica that is infiltrated with a glass forming material, which effectively reduces leaks within the seal. The compressive seal shows approximately a 100-fold reduction in leak rates compared with previously developed hybrid seals after from 10 to about 40 thermal cycles under a compressive stress of from 50 psi to 100 psi at temperatures in the range from 600.degree. C. to about 850.degree. C.

  4. Bellow seal and anchor

    DOEpatents

    Mansure, Arthur J.

    2001-01-01

    An annular seal is made of a collapsible bellows. The bellows can function as an anchor or a seal and is easily set into position using relative component movement. The bellows folds can be slanted and their outer sealing edges can have different profiles to meet expected conditions. The bellows is expanded for insertion to reduce its outer dimension and sets by compaction as a result of relative movement. The bellows can be straight or tapered and is settable with a minimal axial force.

  5. Gland With Cantilever Seal

    NASA Technical Reports Server (NTRS)

    Melton, Patrick B.

    1989-01-01

    Single-piece gland forms tight seal on probe or tube containing liquid or gas at high pressure. Gland and probe align as assembled by simple torquing procedure. Disconnected easily and reused at same site. Made from any of wide variety of materials so compatible with application. Cantilever ring at top of gland bites into wall of tube or probe, sealing it. Wall of tube or probe must be thick enough to accommodate deformation without rupturing. Maximum deformation designed in coordination with seating and deformation of boss or conical seal.

  6. SEALING SIMULATED LEAKS

    SciTech Connect

    Michael A. Romano

    2004-09-01

    This report details the testing equipment, procedures and results performed under Task 7.2 Sealing Simulated Leaks. In terms of our ability to seal leaks identified in the technical topical report, Analysis of Current Field Data, we were 100% successful. In regards to maintaining seal integrity after pigging operations we achieved varying degrees of success. Internal Corrosion defects proved to be the most resistant to the effects of pigging while External Corrosion proved to be the least resistant. Overall, with limitations, pressure activated sealant technology would be a viable option under the right circumstances.

  7. Engine sealing and lubrication systems

    NASA Technical Reports Server (NTRS)

    Zuk, J.

    1975-01-01

    Engine sealing programs are discussed which are directed toward the two major classes of engine seals: engine shaft seals and primary gas path seals. In addition, some concepts and results from fundamental lubrication research, as it pertains to the lubrication of bearings, are presented.

  8. Tamper indicating seal

    DOEpatents

    Romero, Juan A [Albuquerque, NM; Walker, Charles A [Albuquerque, NM; Blair, Dianna S [Albuquerque, NM; Bodmer, Connie C [Albuquerque, NM

    2012-05-29

    Seals have a flexible wire that can be looped through a hasp-like device. The seals include a body having a recess, a plug insertable into the recess and a snap ring for fastening the plug to the body. The plug and/or body can have access holes for inserting the wire into the recess. "Teeth" on the outer diameter and through-holes through the thickness of the snap ring allow for passing the ends of the flexible wire from the recess through the snap ring. The ends of the wire can be folded back over the snap ring and into engagement with the teeth. Assembly of the seal causes the ends of the wire to be securely fastened between the teeth of the snap ring and the sidewall of the recess. Seals can include a plug and/or body made of a frangible material such as glass, ceramic, glass-ceramic or brittle polymer.

  9. Gas turbine sealing apparatus

    SciTech Connect

    Marra, John Joseph; Wessell, Brian J.; Liang, George

    2013-03-05

    A sealing apparatus in a gas turbine. The sealing apparatus includes a seal housing apparatus coupled to a disc/rotor assembly so as to be rotatable therewith during operation of the gas turbine. The seal housing apparatus comprises a base member, a first leg portion, a second leg portion, and spanning structure. The base member extends generally axially between forward and aft rows of rotatable blades and is positioned adjacent to a row of stationary vanes. The first leg portion extends radially inwardly from the base member and is coupled to the disc/rotor assembly. The second leg portion is axially spaced from the first leg portion, extends radially inwardly from the base member, and is coupled to the disc/rotor assembly. The spanning structure extends between and is rigidly coupled to each of the base member, the first leg portion, and the second leg portion.

  10. Flexible cloth seal assembly

    DOEpatents

    Bagepalli, B.S.; Taura, J.C.; Aksit, M.F.; Demiroglu, M.; Predmore, D.R.

    1999-06-29

    A seal assembly is described having a flexible cloth seal which includes a shim assemblage surrounded by a cloth assemblage. A first tubular end portion, such as a gas turbine combustor, includes a longitudinal axis and has smooth and spaced-apart first and second surface portions defining a notch there between which is wider at its top than at its bottom and which extends outward from the axis. The second surface portion is outside curved, and a first edge of the cloth seal is positioned in the bottom of the notch. A second tubular end portion, such as a first stage nozzle, is located near, spaced apart from, and coaxially aligned with, the first tubular end portion. The second tubular end portion has a smooth third surface portion which surrounds at least a portion of the first tubular end portion and which is contacted by the cloth seal. 7 figs.

  11. Rotatable seal assembly

    DOEpatents

    Logan, Clinton M.; Garibaldi, Jack L.

    1982-01-01

    An assembly is provided for rotatably supporting a rotor on a stator so that vacuum chambers in the rotor and stator remain in communication while the chambers are sealed from ambient air, which enables the use of a ball bearing or the like to support most of the weight of the rotor. The apparatus includes a seal device mounted on the rotor to rotate therewith, but shiftable in position on the rotor while being sealed to the rotor as by an O-ring. The seal device has a flat face that is biased towards a flat face on the stator, and pressurized air is pumped between the faces to prevent contact between them while spacing them a small distance apart to avoid the inflow of large amounts of air between the faces and into the vacuum chambers.

  12. Flexible cloth seal assembly

    DOEpatents

    Bagepalli, Bharat Sampathkumar; Taura, Joseph Charles; Aksit, Mahmut Faruk; Demiroglu, Mehmet; Predmore, Daniel Ross

    1999-01-01

    A seal assembly having a flexible cloth seal which includes a shim assemblage surrounded by a cloth assemblage. A first tubular end portion, such as a gas turbine combustor, includes a longitudinal axis and has smooth and spaced-apart first and second surface portions defining a notch therebetween which is wider at its top than at its bottom and which extends outward from the axis. The second surface portion is outside curved, and a first edge of the cloth seal is positioned in the bottom of the notch. A second tubular end portion, such as a first stage nozzle, is located near, spaced apart from, and coaxially aligned with, the first tubular end portion. The second tubular end portion has a smooth third surface portion which surrounds at least a portion of the first tubular end portion and which is contacted by the cloth seal.

  13. Turbine seal assembly

    SciTech Connect

    Little, David A.

    2013-04-16

    A seal assembly that limits gas leakage from a hot gas path to one or more disc cavities in a turbine engine. The seal assembly includes a seal apparatus that limits gas leakage from the hot gas path to a respective one of the disc cavities. The seal apparatus comprises a plurality of blade members rotatable with a blade structure. The blade members are associated with the blade structure and extend toward adjacent stationary components. Each blade member includes a leading edge and a trailing edge, the leading edge of each blade member being located circumferentially in front of the blade member's corresponding trailing edge in a direction of rotation of the turbine rotor. The blade members are arranged such that a space having a component in a circumferential direction is defined between adjacent circumferentially spaced blade members.

  14. Ingestion resistant seal assembly

    SciTech Connect

    Little, David A

    2011-12-13

    A seal assembly limits gas leakage from a hot gas path to one or more disc cavities in a gas turbine engine. The seal assembly includes a seal apparatus associated with a blade structure including a row of airfoils. The seal apparatus includes an annular inner shroud associated with adjacent stationary components, a wing member, and a first wing flange. The wing member extends axially from the blade structure toward the annular inner shroud. The first wing flange extends radially outwardly from the wing member toward the annular inner shroud. A plurality of regions including one or more recirculation zones are defined between the blade structure and the annular inner shroud that recirculate working gas therein back toward the hot gas path.

  15. Conduit sealing system

    SciTech Connect

    Weinberg, R.A.

    1984-02-28

    The invention relates to an annular seal system designed for high pressure applications in subterranean wells. The annular seal system comprises a vertical stack of subassemblies. Each subassembly incorporates an annular sealing element formed from an elastomeric material, such as a perfluoroelastomer, which is provided with a truncated pear-shaped cross-sectional configuration having reversely curved axial side surfaces. The sealing element is abutted on each axial side by a uniform thickness annular bearing element formed from a thermoplastic such as a polyphenylene sulfide resin having good bearing properties. Each of the thermoplastic bearing elements is in turn abutted by an annular metallic restraining element having correspondingly shaped reversely curved axial side surfaces and defining an inverted truncated pear-shaped cross-sectional configuration.

  16. Current developments in brush seals

    NASA Technical Reports Server (NTRS)

    Loewenthal, Bob

    1994-01-01

    The objective of the brush seal development program is to develop a comprehensive design methodology for brush seals using application requirements from engine manufacturers and experimental characterization of seal design and tribological pairs. The goals are to substantially lower leakage compared to labyrinth seals, seal life consistent with man-rated mission requirements, to investigate single and multiple staged brush seals, temperature up to 1200 F and surface speed up to 900 fps, and pressure drop across the seal of 50 psid. Test results are presented in viewgraph format.

  17. Ultra high vacuum seal arrangement

    DOEpatents

    Flaherty, Robert

    1981-01-01

    Arrangement for demountably sealing two concentric metallic tubes in an ultra high vacuum system which facilitates remote actuation. A tubular seal includes integral spaced lips which circumferentially engage the metallic tubes. The lips plastically deform the metallic tubes by mechanical forces resulting from a martensite to austenite transformation of the tubular seal upon application of a predetermined temperature. The sealing force is released upon application of another temperature which causes a transformation from the stronger austenite to the weaker martensite. Use of a dual acting sealing ring and driving ring circumferentially contacting the sealing ring is particularly applicable to sealing larger diameter concentric metallic members.

  18. Seal system with integral detector

    DOEpatents

    Fiarman, S.

    1982-08-12

    A seal system is disclosed for materials where security is of the essence, such as nuclear materials. The seal is tamper-indicating, indicates changes in environmental conditions that evidence attempts to bypass the seal, is unique and cost effective. The seal system is comprised of a seal where an optical signal is transmitted through a loop, with a detector to read said signal, and one or more additional detectors designed to detect environmental changes, these detectors being operatively associated with the seal so that detection of a break in the optical signal or detection of environmental changes will cause an observable change in the seal.

  19. Self-acting shaft seals

    NASA Technical Reports Server (NTRS)

    Ludwig, L. P.

    1978-01-01

    Self-acting seals are described in detail. The mathematical models for obtaining a seal force balance and the equilibrium operating film thickness are outlined. Particular attention is given to primary ring response (seal vibration) to rotating seat face runout. This response analysis reveals three different vibration models with secondary seal friction being an important parameter. Leakage flow inlet pressure drop and affects of axisymmetric sealing face deformations are discussed. Experimental data on self-acting face seals operating under simulated gas turbine conditions are given. Also a spiral groove seal design operated to 244 m/sec (800 ft/sec) is described.

  20. Core disruptive accident margin seal

    DOEpatents

    Golden, Martin P.

    1979-01-01

    Apparatus for sealing the annulus defined within a substantially cylindrical rotatable riser assembly and plug combination of a nuclear reactor closure head. The apparatus comprises an inflatable sealing mechanism disposed in one portion of the riser assembly near the annulus such that upon inflation the sealing mechanism is radially actuated against the other portion of the riser assembly thereby sealing the annulus. The apparatus further comprises a connecting mechanism which places one end of the sealing mechanism in fluid communication with the reactor cover gas so that overpressurization of the reactor cover gas will increase the radial actuation of the sealing mechanism thus enhancing sealing of the annulus.

  1. Radial pressure flange seal

    DOEpatents

    Batzer, Thomas H.; Call, Wayne R.

    1989-01-01

    This invention provides an all metal seal for vacuum or pressure vessels or systems. This invention does not use gaskets. The invention uses a flange which fits into a matching groove. Fluid pressure is applied in a chamber in the flange causing at least one of the flange walls to radially press against a side of the groove creating the seal between the flange wall and the groove side.

  2. Radial pressure flange seal

    DOEpatents

    Batzer, T.H.; Call, W.R.

    1989-01-24

    This invention provides an all metal seal for vacuum or pressure vessels or systems. This invention does not use gaskets. The invention uses a flange which fits into a matching groove. Fluid pressure is applied in a chamber in the flange causing at least one of the flange walls to radially press against a side of the groove creating the seal between the flange wall and the groove side. 5 figs.

  3. Titanium hermetic seals

    DOEpatents

    Brow, Richard K.; Watkins, Randall D.

    1995-01-01

    Titanium is prenitrided by being heated in a nitrogen environment under conditions which give rise to the formation of a titanium-nitride surface layer on the titanium. Titanium thus prenitrided may be used in electrical components which are hermetically sealed using silicate glasses and standard glass sealing techniques. According to the method of the invention, alkali volatilization and formation of deleterious interfacial silicide are inhibited.

  4. Titanium hermetic seals

    DOEpatents

    Brow, Richard K.; Watkins, Randall D.

    1995-07-04

    Titanium is prenitrided by being heated in a nitrogen environment under conditions which give rise to the formation of a titanium-nitride surface layer on the titanium. Titanium thus prenitrided may be used in electrical components which are hermetically sealed using silicate glasses and standard glass sealing techniques. According to the method of the invention, alkali volatilization and formation of deleterious interfacial silicide are inhibited.

  5. ARID3B Directly Regulates Ovarian Cancer Promoting Genes

    PubMed Central

    Bobbs, Alexander; Gellerman, Katrina; Hallas, William Morgan; Joseph, Stancy; Yang, Chao; Kurkewich, Jeffrey; Cowden Dahl, Karen D.

    2015-01-01

    The DNA-binding protein AT-Rich Interactive Domain 3B (ARID3B) is elevated in ovarian cancer and increases tumor growth in a xenograft model of ovarian cancer. However, relatively little is known about ARID3B's function. In this study we perform the first genome wide screen for ARID3B direct target genes and ARID3B regulated pathways. We identified and confirmed numerous ARID3B target genes by chromatin immunoprecipitation (ChIP) followed by microarray and quantitative RT-PCR. Using motif-finding algorithms, we characterized a binding site for ARID3B, which is similar to the previously known site for the ARID3B paralogue ARID3A. Functionality of this predicted site was demonstrated by ChIP analysis. We next demonstrated that ARID3B induces expression of its targets in ovarian cancer cell lines. We validated that ARID3B binds to an epidermal growth factor receptor (EGFR) enhancer and increases mRNA expression. ARID3B also binds to the promoter of Wnt5A and its receptor FZD5. FZD5 is highly expressed in ovarian cancer cell lines, and is upregulated by exogenous ARID3B. Both ARID3B and FZD5 expression increase adhesion to extracellular matrix (ECM) components including collagen IV, fibronectin and vitronectin. ARID3B-increased adhesion to collagens II and IV require FZD5. This study directly demonstrates that ARID3B binds target genes in a sequence-specific manner, resulting in increased gene expression. Furthermore, our data indicate that ARID3B regulation of direct target genes in the Wnt pathway promotes adhesion of ovarian cancer cells. PMID:26121572

  6. Applauding the performing seal

    SciTech Connect

    Not Available

    1992-03-01

    Whether the culprit is a pump, valve, compressor, or flanged pipe connection, the end result is the same: process-fluid leakage damages the environment and is costly to operators, in terms of lost product, lost production due to downtime, and the replacement of damaged process equipment. Hard-to-handle fluids, such as abrasive slurries of coal and flyash, can damage the seals that contain them, allowing potentially harmful fluids to escape. Even more insidious is the often-invisible escape of volatile organic compounds (VOCs) from equipment that handles liquid petroleum, petroleum by-products and chemicals. This paper reports that seal manufacturers are using their ingenuity to minimize the escape of liquid and gaseous emissions. One approach uses the power of magnetic attraction to create a seal along a rotating pump shaft by positioning magnetic fluids there. Ferrofluidics Corp. starts by creating a magnetic fluid - essentially a colloidal suspension of ultrafine magnetic salts dispersed in a carrier fluid - which is then used as a liquid O-ring seal in conjunction with a mechanical seal. The seal apparatus consists of a doughnut-shaped sandwich (centered around the pump shaft), with a permanent magnet as the middle layer, and a washer of magnetically permeable material on either side.

  7. Nuclear instrumentation cable end seal

    DOEpatents

    Cannon, Collins P.; Brown, Donald P.

    1979-01-01

    An improved coaxial end seal for hermetically sealed nuclear instrumentation cable exhibiting an improved breakdown pulse noise characteristic under high voltage, high temperature conditions. A tubular insulator body has metallized interior and exterior surface portions which are braze sealed to a center conductor and an outer conductive sheath. The end surface of the insulator body which is directed toward the coaxial cable to which it is sealed has a recessed surface portion within which the braze seal material terminates.

  8. Splice variants DNMT3B4 and DNMT3B7 overexpression inhibit cell proliferation in 293A cell line.

    PubMed

    Shao, Guo; Zhang, Ran; Zhang, Shu; Jiang, Shuyuan; Liu, You; Zhang, Wei; Zhang, Yanbo; Li, Jinping; Gong, Kerui; Gong, Keri; Hu, Xin-Rong; Jiang, Shi-Wen

    2013-05-01

    DNA methyltransferase 3B (DNMT3B) is critical in abnormal DNA methylation patterns in cancer cells. Nearly 40 alternatively spliced variants of DNMT3B have been reported. DNMT3B4 and DNMT3B7 are two kinds of splice variants of DNMT3B lacking the conserved methyltransferase motif. In this study, the effect of inactivation of DNMT3B variants, DNMT3B4 and DNMT3B7, on cell proliferation was assessed. pCMV-DNMT3B4 and pCMV-DNMT3B7 recombinant plasmids were developed and stably transfected into 293A cells. 293A cells transfected with plasmid pCMV-DNMT3B4 or pCMV-2B were then treated with G418 to the stable cell lines. After that, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method was used for testing the proliferation level, and flow cytometry was used to test cell cycle distribution of the cell line. The expression of p21 was detected by real-time PCR and Western blot. The methylation status of p21 promoter was detected by methylation-specific PCR (MS-PCR). It was found that DNMT3B4 and DNMT3B7 overexpression could inhibit cell proliferation and increase the expression of p21. Cell cycle analysis demonstrated that inactivation of DNMT3B variants overexpression inhibited cell cycle progression. Inactivation of DNMT3B variants overexpression facilitated p21 expression to delay 293A cell proliferation. These findings indicate that inactivation of DNMT3B variants might play an important role in cell proliferation correlating with the change of p21.

  9. Magnetic shaft seals prevent hazardous leakage from wastewater agitators

    SciTech Connect

    Traino, F.A.

    1985-11-01

    The US Department of Energy's laboratory in Miamisburg, OH, operated by Monsanto Research Corporation, processes approximately 45,000 gallons per week of low-level radioactive wastewater to meet Federal Environmental Protection Agency quality standards. Preventing the spread of radioactive contamination throughout the operating area demands effective sealing of all process piping, valves, pumps, and agitators. Rotating shafts of pumps and agitators installed a the start of operations in 1947 were sealed by stuffing glands with graphite impregnated asbestos packing. These pumps proved to be unsatisfactory. In the mid-1970's, new process pumps with mechanical seals and some with magnetic drives were installed. Later, in January 1979, new agitator shaft drives with double tandem, spring-loaded mechanical seals were installed, maintenance of these pumps was costly. The agitator drive shafts were redesigned to accommodate magnetic seals of the type successfully used in blowers and vacuum/pressure pumps in other plant locations. One inherent advantage of the magnetic seal is that it operates with a face loading as much as 50% less than a conventional spring-loaded mechanical seal. The lower loading by a predetermined uniform magnetic force contributes to long face life. Other advantages include compactness, ease of assembly with only a few parts, and insensitivity to vibration. The magnetic shaft seals installed on the agitator shafts in February 1983 are still in service without any leakage or need for maintenance. Based on current operating data and a projected five-year meantime between failures, the estimated cost benefit of the magnetic seals over spring-loaded mechanical seals over spring-loaded mechanical seals will be $640 vs $2400 respectively per seal, with 60% less downtime for maintenance.

  10. Pressure Actuated Leaf Seals for Improved Turbine Shaft Sealing

    NASA Technical Reports Server (NTRS)

    Grondahl, Clayton

    2006-01-01

    This presentation introduces a shaft seal in which leaf seal elements are constructed from slotted shim material formed and layered into a frusto-conical assembly. Limited elastic deflection of seal leaves with increasing system pressure close large startup clearance to a small, non-contacting, steady state running clearance. At shutdown seal elements resiliently retract as differential seal pressure diminishes. Large seal clearance during startup and shutdown provides a mechanism for rub avoidance. Minimum operating clearance improves performance and non-contacting operation promises long seal life. Design features of this seal, sample calculations at differential pressures up to 2400 psid and benefit comparison with brush and labyrinth seals is documented in paper, AIAA 2005 3985, presented at the Advanced Seal Technology session of the Joint Propulsion Conference in Tucson this past July. In this presentation use of bimetallic leaf material will be discussed. Frictional heating of bimetallic leaf seals during a seal rub can relieve the rub condition to some extent with a change in seal shape. Improved leaf seal rub tolerance is expected with bimetallic material.

  11. Serum chemistry and antibodies against pathogens in antarctic fur seals, Weddell seals, crabeater seals, and Ross seals.

    PubMed

    Tryland, Morten; Nymo, Ingebjørg H; Nielsen, Ole; Nordøy, Erling S; Kovacs, Kit M; Krafft, Bjørn A; Thoresen, Stein I; Åsbakk, Kjetil; Osterrieder, Klaus; Roth, Swaantje J; Lydersen, Christian; Godfroid, Jacques; Blix, Arnoldus S

    2012-07-01

    Information on health parameters, such as antibody prevalences and serum chemistry that can reveal exposure to pathogens, disease, and abnormal physiologic conditions, is scarce for Antarctic seal species. Serum samples from Antarctic fur seals (Arctocephalus gazella, n=88) from Bouvetøya (2000-2001 and 2001-2002), and from Weddell seals (Leptonychotes weddellii, n=20), Ross seals (Ommatophoca rossii, n=20), and crabeater seals (Lobodon carcinophagus, n=9) from the pack-ice off Queen Maud Land, Antarctica (2001) were analyzed for enzyme activity, and concentrations of protein, metabolites, minerals, and cortisol. Adult Antarctic fur seal males had elevated levels of total protein (range 64-99 g/l) compared to adult females and pups (range 52-79 g/l). Antarctic fur seals had higher enzyme activities of creatine kinase, lactate dehydrogenase, and amylase, compared to Weddell, Ross, and crabeater seals. Antibodies against Brucella spp. were detected in Weddell seals (37%), Ross seals (5%), and crabeater seals (11%), but not in Antarctic fur seals. Antibodies against phocine herpesvirus 1 were detected in all species examined (Antarctic fur seals, 58%; Weddell seals, 100%; Ross seals, 15%; and crabeater seals, 44%). No antibodies against Trichinella spp., Toxoplasma, or phocine distemper virus (PDV) were detected (Antarctic fur seals were not tested for PDV antibodies). Antarctic seals are challenged by reduced sea ice and increasing temperatures due to climate change, and increased anthropogenic activity can introduce new pathogens to these vulnerable ecosystems and represent a threat for these animals. Our data provide a baseline for future monitoring of health parameters of these Antarctic seal species, for tracking the impact of environmental, climatic, and anthropogenic changes in Antarctica over time.

  12. Sin3b Interacts with Myc and Decreases Myc Levels*

    PubMed Central

    Garcia-Sanz, Pablo; Quintanilla, Andrea; Lafita, M. Carmen; Moreno-Bueno, Gema; García-Gutierrez, Lucia; Tabor, Vedrana; Varela, Ignacio; Shiio, Yuzuru; Larsson, Lars-Gunnar; Portillo, Francisco; Leon, Javier

    2014-01-01

    Myc expression is deregulated in many human cancers. A yeast two-hybrid screen has revealed that the transcriptional repressor Sin3b interacts with Myc protein. Endogenous Myc and Sin3b co-localize and interact in the nuclei of human and rat cells, as assessed by co-immunoprecipitation, immunofluorescence, and proximity ligation assay. The interaction is Max-independent. A conserved Myc region (amino acids 186–203) is required for the interaction with Sin3 proteins. Histone deacetylase 1 is recruited to Myc-Sin3b complexes, and its deacetylase activity is required for the effects of Sin3b on Myc. Myc and Sin3a/b co-occupied many sites on the chromatin of human leukemia cells, although the presence of Sin3 was not associated with gene down-regulation. In leukemia cells and fibroblasts, Sin3b silencing led to Myc up-regulation, whereas Sin3b overexpression induced Myc deacetylation and degradation. An analysis of Sin3b expression in breast tumors revealed an association between low Sin3b expression and disease progression. The data suggest that Sin3b decreases Myc protein levels upon Myc deacetylation. As Sin3b is also required for transcriptional repression by Mxd-Max complexes, our results suggest that, at least in some cell types, Sin3b limits Myc activity through two complementary activities: Mxd-dependent gene repression and reduction of Myc levels. PMID:24951594

  13. Permeameter studies of water flow through cement and clay borehole seals in granite, basalt and tuff

    SciTech Connect

    South, D.L.; Daemen, J.J.K.

    1986-10-01

    Boreholes near a repository must be sealed to prevent rapid migration of radionuclide-contaminated water to the accessible environment. The objective of this research is to assess the performance of borehole seals under laboratory conditions, particularly with regard to varying stress fields. Flow through a sealed borehole is compared with flow through intact rock. Cement or bentonite seals have been tested in granite, basalt, and welded tuff. The main conclusion is that under laboratory conditions, existing commercial materials can form high quality seals. Triaxial stress changes about a borehole do not significantly affect seal performance if the rock is stiffer than the seal. Temperature but especially moisture variations (drying) significantly degrade the quality of cement seals. Performance partially recovers upon resaturation. A skillfully sealed borehole may be as impermeable as the host rock. Analysis of the influence of relative seal-rock permeabilities shows that a plug with permeability one order of magnitude greater than that of the rock results in a flow increase through the hole and surrounding rock of only 1-1/2 times compared to the undisturbed rock. Since a borehole is only a small part of the total rock mass, the total effect is even less pronounced. The simplest and most effective way to decrease flow through a rock-seal system is to increase the seal length, assuming it can be guaranteed that no dominant by-pass flowpath through the rock exists.

  14. Development of seals for a geothermal downhole intensifier. Progress report

    SciTech Connect

    Captain, K.M.; Harvey, A.C.; Caskey, B.C.

    1985-08-01

    A system using high-velocity fluid jets in conjunction with a rotary diamond bit is currently considered as the best candidate for reducing the cost of drilling geothermal wells. Technical, safety and cost considerations indicate that the required jet supply pressure can best be established by a downhole pressure intensifier. Key intensifier components are the check valve and plunger seals, which must prevent leakage of the high-pressure, high-temperature abrasive fluid (drilling mud). To achieve the required performance, novel ceramic seals are currently being developed. The check valve seal includes a tapered polymeric plug and ceramic stop acting against a ceramic seat. The ceramic plunger seal is a variant of the ''stepped-joint'' piston ring and is designed to minimize contact pressure and abrasive wear. Initial testing of these seals in the laboratory shows encouraging results; design refinement and further testing is in progress. 2 refs., 6 figs., 3 tabs.

  15. Application of asphalt emulsion seals to uranium mill tailings

    SciTech Connect

    Hartley, J.N.; Koehmstedt, P.L.; Esterl, D.J.; Freeman, H.D.; Clark, R.L.

    1980-11-01

    Studies of asphalt emulsion sealants have demonstrated that the sealants are effective in containing radon and other potentially hazardous material within uranium tailings. The laboratory and field studies have further demonstrated that radon exhalation from uranium tailings piles can be reduced by greater than 99% to less than background levels. Field tests at the tailings pile in Grand Junction, Colorado confirmed that an 8-cm admix seal containing 22 wt % asphalt could be effectively applied with a cold-mix paver. Other techniques were successfully tested, including a soil stabilizer and a hot, rubberized asphalt seal that was applied with a distributor truck. After the seals were applied and conpacted, overburden was applied over the seal to protect the seal from ultraviolet degradation. 14 figures.

  16. ALTERNATIVE METHODS OF SEALING PLANAR SOLID OXIDE FUEL CELLS

    SciTech Connect

    Weil, K. Scott; Coyle, Christopher A.; Hardy, John S.; Kim, Jin Yong Y.; Xia, Gordon

    2005-03-01

    One of the key limiting issues in designing and fabricating a high performance planar solid oxide fuel cell (pSOFC) stack is the development of the appropriate materials and techniques for hermetically sealing the metal and ceramic components. There are essentially two standard methods of sealing: (1) by forming a rigid joint or (2) by constructing a compressive “sliding” seal. While short-term success has been achieved with both techniques, it is apparent that to meet the long-term operational needs of stack designers, alternative sealing concepts will need to be conceived. Described below are two alternative pSOFC sealing methods that have been developed at Pacific Northwest National Laboratory.

  17. Shaft seal assembly and method

    NASA Technical Reports Server (NTRS)

    Keba, John E. (Inventor)

    2007-01-01

    A pressure-actuated shaft seal assembly and associated method for controlling the flow of fluid adjacent a rotatable shaft are provided. The seal assembly includes one or more seal members that can be adjusted between open and closed positions, for example, according to the rotational speed of the shaft. For example, the seal member can be configured to be adjusted according to a radial pressure differential in a fluid that varies with the rotational speed of the shaft. In addition, in the closed position, each seal member can contact a rotatable member connected to the shaft to form a seal with the rotatable member and prevent fluid from flowing through the assembly. Thus, the seal can be closed at low speeds of operation and opened at high speeds of operation, thereby reducing the heat and wear in the seal assembly while maintaining a sufficient seal during all speeds of operation.

  18. Frequency of FCGR3B Alleles in Thai Blood Donors

    PubMed Central

    Kaset, Chollanot; Leetrakool, Nipapan; Intharanut, Kamphon

    2013-01-01

    Background Human neutrophil antigens (HNAs) are involved in autoimmune and alloimmune neutropenia and transfusion-related acute lung injury. The HNA-1 system is important in immunogenetics, and allele frequencies have been described in different populations. This study investigated the frequency of FCGR3B alleles encoding HNA-1a, HNA-1b, and HNA-1c among Thai blood donors and compared these frequencies with those previously reported for other populations. Methods Eight hundred DNA samples obtained from unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok, and the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, were included. Samples were simultaneously typed for each FCGR3B allele using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique. Results The frequencies of FCGR3B*1, FCGR3B*2, and FCGR3B*3 alleles in central Thai blood donors were 0.548, 0.452, and 0.004, respectively; only FCGR3B*1 and FCGR3B*2 alleles were found in northern Thai blood donors (0.68 and 0.32, respectively). Compared with other Asian populations, central Thais had higher frequencies of the FCGR3B*2 allele (P<0.001), while the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in northern Thais were similar to those previously reported in Taiwanese and Japanese populations. In contrast, the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in the northern Thai population were statistically different from those observed in central Thai, Korean, German, and Turkish populations. Conclusions FCGR3B allele frequencies were significantly different between central and northern Thai blood donors. Our in-house PCR-SSP method is a simple, cost-effective, and convenient method for FCGR3B allele detection. PMID:24205492

  19. Broadcasting Satellite-3A and -3B (BS-3A and 3B)

    NASA Technical Reports Server (NTRS)

    Horii, M.; Funakawa, K.

    1991-01-01

    The BS-3A and -3B will provide direct color TV broadcasting to the Japanese mainland and remote islands. The satellites will be launched from Tanegashima Space Center by a type H-1 launch vehicle. The coverage will consist of the 26-m antenna and the 34-m antenna as a backup support for the transfer and drift orbits. Maximum support will consist of one 8-hour track per station for a seven day period, plus 23 days of contingency support from all complexes. Information is given in tabular form for Deep Space Network support, frequency assignments, telemetry, command, and tracking support responsibility.

  20. Design guide for helicopter transmission seals

    NASA Technical Reports Server (NTRS)

    Hayden, T. S.; Keller, C. H., Jr.

    1974-01-01

    A detailed approach for the selection and design of seals for helicopter transmissions is presented. There are two major types of seals presently being used and they are lip type seals and mechanical type seals. Lip type seals can be divided in conventional lip seals and hydrodynamic lip seals. Conventional lip seals can be used for slow-speed, low-pressure, low-temperature sealing. Hydrodynamic lip seals although they are as pressure and temperature limited as conventional lip seals, can operate at a higher speed. Mechanical types seals are comprised of face seals and circumferential seals. Face seals are capable of high speed, high pressure, and high temperature. Circumferential seals can be used in high-speed and high-temperature applications, but will leak excessively at moderate pressures. The performance goals of transmission seals are a life that is at least equal to the scheduled overhaul interval of the gearbox component and a leakage rate of near zero.

  1. Seal system with integral detector

    DOEpatents

    Fiarman, Sidney

    1985-01-01

    There is disclosed a seal system for materials where security is of the essence, such as nuclear materials, which is tamper-indicating, which indicates changes in environmental conditions that evidence attempts to by-pass the seal, which is unique and cost effective, said seal system comprised of a seal where an optical signal is transmitted through a loop, with a detector to read said signal, and one or more additional detectors designed to detect environmental changes, these detectors being operatively associated with the seal so that detection of a break in the optical signal or detection of environmental changes will cause an observable change in the seal.

  2. Face-centered-cubic K3B80 and Mg3B80 metals: Covalent and ionic bondings

    NASA Astrophysics Data System (ADS)

    Yan, Qing-Bo; Zheng, Qing-Rong; Su, Gang

    2009-09-01

    By means of first-principles calculations within the density-functional theory, we find that stable face-centered-cubic (fcc) K3B80 and Mg3B80 solids can be formed. For both solids, two possibly stable geometrical phases (identified as phase A and phase B ) with different lattice parameters can exist, where phase A has a lattice parameter smaller than phase B . In phase A , B80 clusters are significantly distorted and two or four intercluster covalent bonds are formed for K3B80 or Mg3B80 , respectively. In phase B , B80 units are slightly distorted and no intercluster covalent bonds exist. The phase A of Mg3B80 bears the largest cohesive energy among them and is more stable than the fcc B80 solid. The charge population analysis shows that K and Mg are ionized and donate electrons to the other boron atoms of K3B80 and Mg3B80 solids. The different ionic radii of K and Mg lead to major geometrical differences between K3B80 and Mg3B80 solids and the competition of the covalent and ionic bondings could explain the emergence of two different geometrical phases for both. The electronic structural calculations reveal that both fcc K3B80 and Mg3B80 solids are metals.

  3. Improving tag/seal technologies: the vulnerability assessment component

    SciTech Connect

    Jones, J.L.

    1996-02-01

    The Department of Energy (DOE), specifically the Office of Nonproliferation and National Security, has sponsored the development of numerous tag and seal technologies for high-security/high-valued applications. One important component in this technology development effort has been the continuous integration of vulnerability assessments. The Idaho National Engineering Laboratory (INEL) has been the lead laboratory for vulnerability assessments of fiber-optic-based tag/seal technologies. This paper presents a brief historical overview and the current status of the DOE high-security tag/seal development program and discusses INEL`s adversarial role and assessment philosophy. Verification testing criteria used to define ``successful`` tampering attempts/attacks are discussed. Finally, the advantages of integrating a vulnerability assessment into the development of commercial security tag/seals are presented.

  4. Dye filled security seal

    DOEpatents

    Wilson, Dennis C. W.

    1982-04-27

    A security seal for providing an indication of unauthorized access to a sealed object includes an elongate member to be entwined in the object such that access is denied unless the member is removed. The elongate member has a hollow, pressurizable chamber extending throughout its length that is filled with a permanent dye under greater than atmospheric pressure. Attempts to cut the member and weld it together are revealed when dye flows through a rupture in the chamber wall and stains the outside surface of the member.

  5. REACTOR COOLANT TUBE SEAL

    DOEpatents

    Morris, W.J.

    1958-12-01

    A plle-flattenlng control element and a fluid seal therefore to permit movement of the element into a liquld contnining region of a neutronlc reactor are described. The device consists of flattened, thin-walled aluminum tubing contalnlng a uniform mixture of thermal neutron absorbing material, and a number of soft rubber closures for the process tubes, having silts capable of passing the flattened elements therethrough, but effectively sealing the process tubes against fluld leaknge by compression of the rubber. The flattened tubing is sufficiently flexible to enable it to conform to the configuratlon of the annular spacing surrounding the fuel elements ln the process tubes.

  6. Method of sealing

    DOEpatents

    Groh, Edward F.; Cassidy, Dale A.

    1978-01-01

    A thermocouple lead or other small diameter wire, cable or tube is passed through a thin material such as sheet metal and sealed thereinto by drawing complementary longitudinally angled, laterally rounded grooves terminating at their base ends in a common plane in both sides of the thin material with shearing occuring at the deep end faces thereof to form a rounded opening in the thin material substantially perpendicular to the plane of the thin material, passing a thermocouple lead or similar object through the opening so formed and sealing the opening with a sealant which simultaneously bonds the lead to the thin material.

  7. Sealing coupling. [LMFBR

    DOEpatents

    Pardini, J.A.; Brubaker, R.C.; Rusnak, J.J.

    1982-09-20

    Disclosed is a remotely operable releasable sealing coupling which provides fluid-tight joinder of upper and a lower conduit sections. Each conduit section has a concave conical sealing surface adjacent its end portion. A tubular sleeve having convex spherical ends is inserted between the conduit ends to form line contact with the concave conical end portions. An inwardly projecting lip located at one end of the sleeve cooperates with a retaining collar formed on the upper pipe end to provide swivel capture for the sleeve. The upper conduit section also includes a tapered lower end portion which engages the inside surface of the sleeve to limit misalignment of the connected conduit sections.

  8. Structural Design and Sealing Performance Analysis of Biomimetic Sealing Ring

    PubMed Central

    Han, Chuanjun

    2015-01-01

    In order to reduce the failure probability of rubber sealing rings in reciprocating dynamic seal, a new structure of sealing ring based on bionics was designed. The biomimetic ring has three concave ridges and convex bulges on each side which are very similar to earthworms. Bulges were circularly designed and sealing performances of the biomimetic ring in both static seal and dynamic seal were simulated by FEM. In addition, effects of precompression, medium pressure, speed, friction coefficient, and material parameters on sealing performances were discussed. The results show that von Mises stress of the biomimetic sealing ring distributed symmetrically in no-pressure static sealing. The maximum von Mises stress appears on the second bulge of the inner side. High contact stress concentrates on left bulges. Von Mises stress distribution becomes uneven under medium pressure. Both von Mises stress and contact stress increase when precompression, medium pressure, and rubber hardness increase in static sealing. Biomimetic ring can avoid rolling and distortion in reciprocating dynamic seal, and its working life is much longer than O-ring and rectangular ring. The maximum von Mises stress and contact stress increase with the precompression, medium pressure, rubber hardness, and friction coefficient in reciprocating dynamic seal. PMID:27019582

  9. Tuberculosis in wild seals and characterisation of the seal bacillus.

    PubMed

    Cousins, D V; Williams, S N; Reuter, R; Forshaw, D; Chadwick, B; Coughran, D; Collins, P; Gales, N

    1993-03-01

    Tuberculosis was diagnosed in 3 otariid seals found dead on beaches at 3 locations on the south coast of Western Australian between May 1990 and March 1991. This confirms that tuberculosis is present in the 2 native seals (Neophoca cinerea and Arctocephalus forsteri) in Western Australian waters. Mycobacterium sp isolated from the lungs of 2 of the seals were studied to determine the similarity of the strains to each other, to the strains isolated during 1986 from Australian sea lions and New Zealand fur seals kept in captivity at a marine park near Perth, Western Australia, and to a strain isolated in 1988 from a seal trainer who worked with the infected captive seals for 3 years. After restriction endonuclease analysis (REA) with the endonucleases Bst EII, Bcl I and Pvu II, one of the wild seal strains appeared to have identical DNA fragment patterns to the strains from the captive seals and the seal trainer. The other wild seal isolate had identical REA profiles using Bst EII and Bcl I, but a minor difference was detected using Pvu II. Differences in these isolates were more clearly seen in restriction fragment length polymorphisms after hybridisation with two DNA probes. The secretory protein MPB70, present in M bovis, was not detected in wild seal isolates using sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blotting techniques. Analysis of protein and DNA fragment profiles indicated that seal tuberculosis isolates form a unique cluster within the M tuberculosis complex.

  10. INEL storage facility for sealed sources from the commercial sector

    SciTech Connect

    Kingsford, C.O.; Satterthwaite, B.C.

    1994-08-01

    Commercially owned sealed radiation sources determine by the US Nuclear Regulatory Commission to be a public health or safety hazard are accepted by the US Department of Energy, under the Atomic Energy Act of 1954, as material for reuse of recycle. To implement this policy, the sealed sources must be stored until proper disposition is determined. This report documents the investigation and selection process undertaken to locate a suitable storage facility at the Idaho National Engineering Laboratory.

  11. Static seal for turbine engine

    DOEpatents

    Salazar, Santiago; Gisch, Andrew

    2014-04-01

    A seal structure for a gas turbine engine, the seal structure including first and second components located adjacent to each other and forming a barrier between high and low pressure zones. A seal cavity is defined in the first and second components, the seal cavity extending to either side of an elongated gap extending generally in a first direction between the first and second components. A seal member is positioned within the seal cavity and spans across the elongated gap. The seal member includes first and second side edges extending into each of the components in a second direction transverse to the first direction, and opposing longitudinal edges extending between the side edges generally parallel to the first direction. The side edges include a groove formed therein for effecting a reduction of gas flow around the seal member at the side edges.

  12. Seal For Precooling A Turbopump

    NASA Technical Reports Server (NTRS)

    Owen, Samuel S.; Mulready, R.C.

    1988-01-01

    Diaphragm reduces misalignment. Rotary seal retains precooling fluid in pump section of cryogenic turbopump, preventing fluid from entering turbine section. Precooling fluid held in pump section of turbopump by knife-edge labyrinth seal on diaphragm.

  13. Sealing micropores in thin castings

    NASA Technical Reports Server (NTRS)

    Mersereau, G. A.; Nitzschke, G. O.; Ochs, H. L.; Sutch, F. S.

    1981-01-01

    Microscopic pores in thin-walled aluminum castings are sealed by impregnation pretreatment. Technique was developed for investment castings used in hermetically sealed chassic for electronic circuitry. Excessively high leakage rates were previously measured in some chassis.

  14. Coating for hot sliding seals

    NASA Technical Reports Server (NTRS)

    Stock, J.

    1979-01-01

    Heat resistant paint is effective surface coating for sliding seals that must operate at elevated temperatures. Economical paint is easy to apply, offers minimal friction, and improves reliability of seals.

  15. Feather seal slot for vanes

    SciTech Connect

    Del Mastro, B. P.; Eckart, F.

    1985-10-22

    The slots for accommodating feather seals in the turbine vanes of a gas turbine engine has the end thereof sealed off by use of weld wire inserted into the slot and simultaneously welded and cut to the required length.

  16. DNMT3B7, a truncated DNMT3B isoform expressed in human tumors, disrupts embryonic development and accelerates lymphomagenesis

    PubMed Central

    Shah, Mrinal Y.; Vasanthakumar, Aparna; Barnes, Natalie Y.; Figueroa, Maria E.; Kamp, Anna; Hendrick, Christopher; Ostler, Kelly R.; Davis, Elizabeth M.; Lin, Shang; Anastasi, John; Le Beau, Michelle M.; Moskowitz, Ivan; Melnick, Ari; Pytel, Peter; Godley, Lucy A.

    2010-01-01

    Epigenetic changes are among the most common alterations observed in cancer cells, yet the mechanism by which cancer cells acquire and maintain abnormal DNA methylation patterns is not understood. Cancer cells have an altered distribution of DNA methylation and express aberrant DNA methyltransferase 3B transcripts, which encode truncated proteins, some of which lack the C-terminal catalytic domain. To test if a truncated DNMT3B isoform disrupts DNA methylation in vivo, we constructed two lines of transgenic mice expressing DNMT3B7, a truncated DNMT3B isoform commonly found in cancer cells. DNMT3B7 transgenic mice exhibit altered embryonic development, including lymphopenia, craniofacial abnormalities, and cardiac defects, similar to Dnmt3b-deficient animals, but rarely develop cancer. However, when DNMT3B7 transgenic are bred with Eμ-Myc transgenic mice, which model aggressive B cell lymphoma, DNMT3B7 expression increases the frequency of mediastinal lymphomas in Eμ-Myc animals. Eμ-Myc/DNMT3B7 mediastinal lymphomas have more chromosomal rearrangements, increased global DNA methylation levels, and more locus-specific perturbations in DNA methylation patterns compared to Eμ-Myc lymphomas. These data represent the first in vivo modeling of cancer-associated DNA methylation changes and suggest that truncated DNMT3B isoforms contribute to the re-distribution of DNA methylation characterizing virtually every human tumor. PMID:20587527

  17. Separation and Sealing of a Sample Container Using Brazing

    NASA Technical Reports Server (NTRS)

    Bar-Cohen, Yoseph; Rivellini, Tommaso P.; Wincentsen, James E.; Gershman, Robert

    2007-01-01

    A special double-wall container and a process for utilizing the container are being developed to enable (1) acquisition of a sample of material in a dirty environment that may include a biological and/or chemical hazard; (2) sealing a lid onto the inner part of the container to hermetically enclose the sample; (3) separating the resulting hermetic container from the dirty environment; and (4) bringing that hermetic container, without any biological or chemical contamination of its outer surface, into a clean environment. The process is denoted S(exp 3)B (separation, seaming, and sealing using brazing) because sealing of the sample into the hermetic container, separating the container from the dirty environment, and bringing the container with a clean outer surface into the clean environment are all accomplished simultaneously with a brazing operation.

  18. Self-stabilizing radial face seal

    NASA Technical Reports Server (NTRS)

    Etsion, I. (Inventor)

    1981-01-01

    A self-stabilizing radial face seal comprises an axial member and a primary seal ring juxtapositioned to a seal seat. At least one primary seal ring and seal seat unit is affixed to the axial member so as to rotate with it. The primary seal ring has a front face which opposes a face of the seal seat. The seal has both high-pressure and low-pressure regions of fluid, and seal seat is provided with a porous ring-like circumferential structure in the face of the seal seat opposite the front face of the primary seal ring.

  19. Rendezvous radar requirements analysis for mission 3B

    NASA Technical Reports Server (NTRS)

    Hutchison, W. L.; Jones, A. K.

    1975-01-01

    Data are presented verifying the compatibility of currently proposed rendezvous radar measurement accuracies with Mission 3B rendezvous requirements. In addition, data presented indicate a potential for increasing the acceptable time lag between termination of thrusting and availability of accurate measurement data. Additional investigation is recommended to define any acceptable time lag above the current proposed value. Finally, Mission 3B rendezvous performance is shown to be sensitive to variations in the relative downrange position dispersions at insertion. It is therefore recommended that insertion relative state dispersions used in studies of 3B rendezvous be reviewed when results of 3B ascent dispersion studies are available.

  20. Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue

    PubMed Central

    Ahmad, Faiyaz; Chung, Youn Wook; Tang, Yan; Hockman, Steven C.; Liu, Shiwei; Khan, Yusuf; Huo, Kevin; Billings, Eric; Amar, Marcelo J.; Remaley, Alan T.; Manganiello, Vincent C.

    2016-01-01

    Activation of inflammation in white adipose tissue (WAT), includes infiltration/expansion of WAT macrophages, contributes pathogenesis of obesity, insulin resistance, and metabolic syndrome. The inflammasome comprises an intracellular sensor (NLR), caspase-1 and the adaptor ASC. Inflammasome activation leads to maturation of caspase-1 and processing of IL1β, contributing to many metabolic disorders and directing adipocytes to a more insulin-resistant phenotype. Ablation of PDE3B in WAT prevents inflammasome activation by reducing expression of NLRP3, caspase-1, ASC, AIM2, TNFα, IL1β and proinflammatory genes. Following IP injection of lipopolysaccharide (LPS), serum levels of IL1β and TNFα were reduced in PDE3B−/−mice compared to WT. Activation of signaling cascades, which mediate inflammasome responses, were modulated in PDE3B−/−mice WAT, including smad, NFAT, NFkB, and MAP kinases. Moreover, expression of chemokine CCL2, MCP-1 and its receptor CCR2, which play an important role in macrophage chemotaxis, were reduced in WAT of PDE3B−/−mice. In addition, atherosclerotic plaque formation was significantly reduced in the aorta of apoE−/−/PDE3B−/−and LDL-R−/−/PDE3B−/−mice compared to apoE−/−and LDL-R−/−mice, respectively. Obesity-induced changes in serum-cholesterol were blocked in PDE3B−/−mice. Collectively, these data establish a role for PDE3B in modulating inflammatory response, which may contribute to a reduced inflammatory state in adipose tissue. PMID:27321128

  1. GOLD PRESSURE VESSEL SEAL

    DOEpatents

    Smith, A.E.

    1963-11-26

    An improved seal between the piston and die member of a piston-cylinder type pressure vessel is presented. A layer of gold, of sufficient thickness to provide an interference fit between the piston and die member, is plated on the contacting surface of at least one of the members. (AEC)

  2. Piston rod seal

    DOEpatents

    Lindskoug, Stefan

    1984-01-01

    In a piston rod seal of the type comprising a gland through which the piston rod is passed the piston is provided with a sleeve surrounding the piston rod and extending axially so as to axially partly overlap the gland when the piston is in its bottom dead center position.

  3. "The Seventh Seal."

    ERIC Educational Resources Information Center

    Palmer, Peter M.

    1969-01-01

    The significance of Bergman's "Seventh Seal" lies not in the speeches nor in the actions of the central characters but rather in the film's form, its totality created by the emotive elements of imagery and sound together with the intellectual elements of actions and words. The scene-units are related to a central motif (the opening of…

  4. Gas path seal

    NASA Technical Reports Server (NTRS)

    Bill, R. C.; Johnson, R. D. (Inventor)

    1979-01-01

    A gas path seal suitable for use with a turbine engine or compressor is described. A shroud wearable or abradable by the abrasion of the rotor blades of the turbine or compressor shrouds the rotor bades. A compliant backing surrounds the shroud. The backing is a yieldingly deformable porous material covered with a thin ductile layer. A mounting fixture surrounds the backing.

  5. Composite seal for turbomachinery

    NASA Technical Reports Server (NTRS)

    Bill, R. C.; Ludwig, L. P. (Inventor)

    1980-01-01

    A gas path seal suitable for use with a turbine engine or compressor is provided. A shroud wearable or abradable by the abrasion of the rotor blades of the turbine or compressor shrouds the rotor blades. A compliant backing surrounds the shroud. The backing is a compliant material covered with a thin ductile layer. A mounting fixture surrounds the backing.

  6. Composite seal for turbomachinery

    NASA Technical Reports Server (NTRS)

    Bill, R. C.; Ludwig, L. P. (Inventor)

    1981-01-01

    A gas path seal suitable for use with a turbine engine or compressor is provided. A shroud wearable or abradable by the abrasion of the rotor blades of the turbine or compressor shrouds the rotor blades. A compliant backing surrounds the shroud. The backing is a yieldingly deformable porous material covered with a thin ductile layer. A mounting fixture surrounds the backing.

  7. Ceramic to metal seal

    DOEpatents

    Snow, Gary S.; Wilcox, Paul D.

    1976-01-01

    Providing a high strength, hermetic ceramic to metal seal by essentially heating a wire-like metal gasket and a ceramic member, which have been chemically cleaned, while simultaneously deforming from about 50 to 95 percent the metal gasket against the ceramic member at a temperature of about 30 to 75 percent of the melting temperature of the metal gasket.

  8. Seal for sodium sulfur battery

    DOEpatents

    Topouzian, Armenag; Minck, Robert W.; Williams, William J.

    1980-01-01

    This invention is directed to a seal for a sodium sulfur battery in which the sealing is accomplished by a radial compression seal made on a ceramic component of the battery which separates an anode compartment from a cathode compartment of the battery.

  9. Rim-Supported Turbine Seal

    NASA Technical Reports Server (NTRS)

    Longenecker, Kent O.

    1988-01-01

    Interstage seal accommodates large pressure drop across vane stage. Sealing surfaces close to inner diameter of gas-flow path. Two blade stages supported by single disk, broached over entire width of rim. Seal concept developed for small rocket turbines as liquid-oxygen pumps. Well suited to turbines with high pressure drops across vane stages.

  10. Ultrasonic dip seal maintenance system

    DOEpatents

    Poindexter, Allan M.; Ricks, Herbert E.

    1978-01-01

    A system for removing impurities from the surfaces of liquid dip seals and or wetting the metal surfaces of liquid dip seals in nuclear components. The system comprises an ultrasonic transducer that transmits ultrasonic vibrations along an ultrasonic probe to the metal and liquid surfaces of the dip seal thereby loosening and removing those impurities.

  11. Ceramic-Cord Gas Seal

    NASA Technical Reports Server (NTRS)

    Etzel, C. W.

    1983-01-01

    High-temperature gasket material seals at temperatures above 1,100 degrees C. Concentric exhaust pipes are typical of applications in which ceramic-cord seals might be used. Cord is crushed to form seal between inner and outer pipes when inner pipe is expanded into place. Typical applications include engine exhaust ducts or hot pipes passing through firewalls.

  12. Increased DNA methylation of Dnmt3b targets impairs leukemogenesis.

    PubMed

    Schulze, Isabell; Rohde, Christian; Scheller-Wendorff, Marina; Bäumer, Nicole; Krause, Annika; Herbst, Friederike; Riemke, Pia; Hebestreit, Katja; Tschanter, Petra; Lin, Qiong; Linhart, Heinz; Godley, Lucy A; Glimm, Hanno; Dugas, Martin; Wagner, Wolfgang; Berdel, Wolfgang E; Rosenbauer, Frank; Müller-Tidow, Carsten

    2016-03-24

    The de novo DNA methyltransferases Dnmt3a and Dnmt3b are of crucial importance in hematopoietic stem cells. Dnmt3b has recently been shown to play a role in genic methylation. To investigate how Dnmt3b-mediated DNA methylation affects leukemogenesis, we analyzed leukemia development under conditions of high and physiological methylation levels in a tetracycline-inducible knock-in mouse model. High expression of Dnmt3b slowed leukemia development in serial transplantations and impaired leukemia stem cell (LSC) function. Forced Dnmt3b expression induced widespread DNA hypermethylation inMyc-Bcl2-induced leukemias, preferentially at gene bodies.MLL-AF9-induced leukemogenesis showed much less pronounced DNA hypermethylation upon Dnmt3b expression. Nonetheless, leukemogenesis was delayed in both models with a shared core set of DNA hypermethylated regions and suppression of stem cell-related genes. Acute myeloid leukemia patients with high expression of Dnmt3b target genes showed inferior survival. Together, these findings indicate a critical role for Dnmt3b-mediated DNA methylation in leukemia development and maintenance of LSC function.

  13. Significance of DNMT3b in oral cancer.

    PubMed

    Chen, Wen-Cheng; Chen, Miao-Fen; Lin, Paul-Yang

    2014-01-01

    The aim of this study was to explore specific molecular markers that could lead to new insights into the identification of innovative treatments. The role of DNMT3b and its predictive power in the prognosis of oral cancer were identified. Human oral cancer cell lines including SCC4 and SCC25 were selected for cellular experiments. Changes in tumor growth, aggressiveness and the responsible signaling pathway were investigated in vitro and in vivo. Furthermore, 125 oral cancer tissue specimens were analyzed using immunohistochemical staining on tissue microarray slides, and correlations calculated between the level of DNMT3b and the clinical outcome of patients. Our data revealed that inhibition of DNMT3b resulted in slower tumor growth, attenuated tumor invasion ability and epithelial mesenchymal transition, as determined by in vitro and in vivo experiments. Activated IL-6 signaling might be responsible to the induction of DNMT3b overexpression on oral cancer. Regarding clinical data, the incidence of DNMT3b immunoreactivity in oral cancer specimens was significantly higher than in non-malignant epithelium, and positively linked to expression of IL-6. Furthermore, expression of DNMT3b was significantly linked with the risk of lymph node involvement, disease recurrence and shorter survival in patients with pathological stage III-IV oral cancer. In conclusion, IL-6 -DNMT3b axis could be used to predict the prognosis of oral cancer in clinics, and targeting DNMT3b could represent a promising treatment strategy.

  14. Thruster sealing system and apparatus

    NASA Technical Reports Server (NTRS)

    Svejkovsky, Paul A. (Inventor)

    1992-01-01

    A thruster nozzle sealing system and apparatus is provided for protection of spacecraft thruster motors. The system includes a sealing plug, a sealing plug insertion tool, an outer cover, an outer cover attachment, and a ferry flight attachment. The sealing plug prevents moisture from entering the thruster engine so as to prevent valve failure. The attachments are interchangeably connectable with the sealing plug. The ferry flight attachment is used during air transportation of the spacecraft, and the outer cover attachment is used during storage and service of the spacecraft. The outer cover provides protection to the thruster nozzle from mechanical damage.

  15. P3b, consciousness, and complex unconscious processing.

    PubMed

    Silverstein, Brian H; Snodgrass, Michael; Shevrin, Howard; Kushwaha, Ramesh

    2015-12-01

    How can perceptual consciousness be indexed in humans? Recent work with ERPs suggests that P3b, a relatively late component, may be a neural correlate of consciousness (NCC). This proposal dovetails with currently prevailing cognitive theory regarding the nature of conscious versus unconscious processes, which holds that the latter are simple and very brief, whereas consciousness is ostensibly required for more durable, complex cognitive processing. Using a P3b oddball paradigm, we instead show that P3b and even later, related slow wave activity occur under rigorously subliminal conditions. Additional principal component analysis (PCA) further differentiated the presence of both P3a and P3b components, demonstrating that the latter indeed occurred subliminally. Collectively, our results suggest that complex, sustained cognitive processing can occur unconsciously and that P3b is not an NCC after all.

  16. Seals Having Textured Portions for Protection in Space Environments

    NASA Technical Reports Server (NTRS)

    Daniels, Christopher (Inventor); Garafolo, Nicholas (Inventor)

    2016-01-01

    A sealing construct for a space environment includes a seal-bearing object, a seal on the seal-bearing object, and a seal-engaging object. The seal includes a seal body having a sealing surface, and a textured pattern at the sealing surface, the textured pattern defining at least one shaded channel surface. The seal-engaging object is selectively engaged with the seal-bearing object through the seal. The seal-engaging object has a sealing surface, wherein, when the seal-engaging object is selectively engaged with the seal-bearing object, the sealing surface of the seal-engaging object engages the sealing surface of the seal, and the seal is compressed between the seal-bearing object and the seal-engaging object such that at least one shaded channel surface engages the sealing surface of the seal-engaging object.

  17. Hermetic Seal Leak Detection Apparatus

    NASA Technical Reports Server (NTRS)

    Kelley, Anthony R. (Inventor)

    2013-01-01

    The present invention is a hermetic seal leak detection apparatus, which can be used to test for hermetic seal leaks in instruments and containers. A vacuum tight chamber is created around the unit being tested to minimize gas space outside of the hermetic seal. A vacuum inducing device is then used to increase the gas chamber volume inside the device, so that a slight vacuum is pulled on the unit being tested. The pressure in the unit being tested will stabilize. If the stabilized pressure reads close to a known good seal calibration, there is not a leak in the seal. If the stabilized pressure reads closer to a known bad seal calibration value, there is a leak in the seal. The speed of the plunger can be varied and by evaluating the resulting pressure change rates and final values, the leak rate/size can be accurately calculated.

  18. Regenerator cross arm seal assembly

    DOEpatents

    Jackman, Anthony V.

    1988-01-01

    A seal assembly for disposition between a cross arm on a gas turbine engine block and a regenerator disc, the seal assembly including a platform coextensive with the cross arm, a seal and wear layer sealingly and slidingly engaging the regenerator disc, a porous and compliant support layer between the platform and the seal and wear layer porous enough to permit flow of cooling air therethrough and compliant to accommodate relative thermal growth and distortion, a dike between the seal and wear layer and the platform for preventing cross flow through the support layer between engine exhaust and pressurized air passages, and air diversion passages for directing unregenerated pressurized air through the support layer to cool the seal and wear layer and then back into the flow of regenerated pressurized air.

  19. Bellows sealed plug valve

    DOEpatents

    Dukas, Jr., Stephen J.

    1990-01-01

    A bellows sealed plug valve includes a valve body having an inlet passage and an outlet passage, a valve chamber between the inlet and outlet passages. A valve plug has substantially the same shape as the valve chamber and is rotatably disposed therein. A shaft is movable linearly in response to a signal from a valve actuator. A bellows is sealingly disposed between the valve chamber and the valve actuator and means are located between the bellows and the valve plug for converting linear movement of the shaft connected to the valve actuator to rotational movement of the plug. Various means are disclosed including helical thread mechanism, clevis mechanism and rack and pinion mechanism, all for converting linear motion to rotational motion.

  20. Gas turbine sealing apparatus

    SciTech Connect

    Wiebe, David J; Wessell, Brian J; Ebert, Todd; Beeck, Alexander; Liang, George; Marussich, Walter H

    2013-02-19

    A gas turbine includes forward and aft rows of rotatable blades, a row of stationary vanes between the forward and aft rows of rotatable blades, an annular intermediate disc, and a seal housing apparatus. The forward and aft rows of rotatable blades are coupled to respective first and second portions of a disc/rotor assembly. The annular intermediate disc is coupled to the disc/rotor assembly so as to be rotatable with the disc/rotor assembly during operation of the gas turbine. The annular intermediate disc includes a forward side coupled to the first portion of the disc/rotor assembly and an aft side coupled to the second portion of the disc/rotor assembly. The seal housing apparatus is coupled to the annular intermediate disc so as to be rotatable with the annular intermediate disc and the disc/rotor assembly during operation of the gas turbine.

  1. Development of a Cryogenic Gate Valve with Robust Sealing

    NASA Astrophysics Data System (ADS)

    Hatfield, W. H.; Sass, J. P.

    2008-03-01

    A soft-seal cryogenic gate valve has been designed, fabricated, and demonstrated by the Cryogenics Test Laboratory (CTL) at the Kennedy Space Center (KSC), featuring a robust sealing technology and a high flow coefficient (Cv). The robust seal design incorporates novel retracting seal-retaining disks to prevent soft-seal abrasive wear and damage from the valve body hard seat. Advanced materials were incorporated into the design, including fluorinated ethylene propylene (FEP) standard seal material and porous sponge polytetrafluoroethylene (PTFE) optional seal material. Titanium nitride coating was applied to the 316 stainless-steel surfaces that are subject to wearing or galling. The valve is suitable for applications from ambient temperature down to liquid nitrogen temperature (77 K), with a seat leakage rate of less than 1 standard-cubic-centimeter-per minute (sccm) per inch of valve diameter. The very low seat leakage, throttling capability, and high flow coefficient (Cv) of the valve make it suitable for block valve applications on standard to very large cryogenic storage tanks or other applications requiring highly reliable full flow cryogenic valves.

  2. Tamper-indicating seals : practices, problems, and standards

    SciTech Connect

    Johnston, R. G.

    2003-01-01

    Tamper-indicating seals have been used by customs officials for over 7,000 years. Today, seals are widely used to help counter theft, smuggling, sabotage, vandalism, terrorism, and espionage. Despite their antiquity and modern widespread use, however, there remains considerable confusion about seals, as well as a lot of misconceptions, wishful thinking, sloppy terminology, and poor practice. The absence of meaningful norms and standards, together with the surprisingly limited amount of research and development (R&D) in the field of tamper detection, has also hindered the effective use of seals. The Vulnerability Assessment Team (VAT) at Los Alamos National Laboratory has intensively studied tamper-indicating seals for the last 12 years. We have engaged in vulnerability assessments, R&D, consulting, and training for over two dozen United States government agencies and private companies, as well as for the International Atomic Energy Agency (IAEA) and Euratom. The VAT has also analyzed over 200 different types of seals in detail. This paper summarizes some of our conclusions, recommendations, and warnings regarding seals and tamper detection.

  3. Sealing Nitrogen Tetroxide Leaks

    NASA Technical Reports Server (NTRS)

    Garrard, George G.; Houston, Donald W.; Scott, Frank D.

    1990-01-01

    Use of Furmanite FSC-N-6B sealant in clam-shell sealing device makes it possible to stop leaks of nitrogen tetroxide through defective or improperly-seated plumbing fittings. Devised to stop leaks in vent line of small rocket motor on Space Shuttle. Also used on plumbing containing hydrazine and other hazardous fluids, and repair withstands severe temperature, vibration, and shock. Leaks stopped in place, without draining or replacement of leaking parts.

  4. APOBEC3B expression in human leptomeninges and meningiomas

    PubMed Central

    Johnson, Mahlon D.; Reeder, Jay E.; O'Connell, Mary

    2016-01-01

    Nucleic acid-editing enzymes of the apolipoprotein B mRNA-editing enzyme (APOBEC) family have been associated with somatic mutation in cancer. However, the role of APOBEC catalytic subunit 3B (APOBEC3B) editing in the pathogenesis of base substitutions in meningiomas is unknown. In the present study, the expression of APOBEC3B was examined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analyses in five fetal and one adult human leptomeninges and 38 meningiomas. Genomic DNA was sequenced using the Illumina Tru-Seq Cancer Panel. Three meningioma primary cultures were also established and treated with cerebrospinal fluid form patients without neurological disease or platelet-derived growth factor-BB (PDGF-BB), prior to evaluation of APOBEC3B expression. By western blotting, APOBEC3B was revealed to be present in 100% of the fetal leptomeninges, and in 88% of World Health Organization grade I, 100% of grade II and 83% of grade III meningiomas tested, but was not different between grades. RT-qPCR revealed no difference in the mRNA expression of APOBEC3B between grades. Sequencing revealed no elevated levels of the C>T mutations that are characteristic of APOBEC3B editing of genomic DNA. Treatment with cerebrospinal fluid and PDGF-BB had no effect on APOBEC3B protein expression in the leptomeningeal or meningioma cells. These findings suggest that the mutations associated with increased APOBEC3B expression may not be central to the pathogenesis of meningiomas. PMID:28101245

  5. Structural applications of Avimid K3B LDF thermoplastic composites

    NASA Astrophysics Data System (ADS)

    Perrella, Andrew P.

    Composite applications on advanced aircraft require lightweight, high performance, tough material systems which are capable of operating at high service temperatures. These composite systems must also be producible and cost effective. Avimid K3B composite materials and related process and part manufacturing technologies offers a unique solutions to these requirements. The objective of this paper is to describe selected Avimid K3B processing approaches such as Long Discontinuous Fiber thermoforming and fusion bonding. A review of the Avimid K3B F-16 Strake Door Joint Development Program is presented. This program successfully developed, built and structurally validated a flight demonstration component using these materials and manufacturing methods.

  6. Preliminary seal design evaluation for the Waste Isolation Pilot Plant

    SciTech Connect

    Stormont, J C

    1988-03-01

    This report presents a preliminary evaluation of design concepts for the eventual sealing of the shafts, drifts, and boreholes at the Waste Isolation Pilot Plant Facility. The purpose of the seal systems is to limit the flow of water into, through, and out of the repository. The principal design strategy involves the consolidation of crushed or granular salt in response to the closure of the excavations in salt. Other candidate seal materials are bentonite, cementitious mixtures, and possibly asphalt. Results from in situ experiments and modeling studies, as well as laboratory materials testing and related industrial experience, are used to develop seal designs for shafts, waste storage panel entryways, non-waste containing drifts, and boreholes. Key elements of the ongoing experimental program are identified. 112 refs., 25 figs., 1 tab.

  7. Navy GTE seal development activity

    NASA Technical Reports Server (NTRS)

    Grala, Carl P.

    1993-01-01

    Under the auspices of the Integrated High Performance Turbine Engine Technology Initiative, the Naval Air Warfare Center conducts advanced development programs for demonstration in the next generation of air-breathing propulsion systems. Among the target technologies are gas path and lube oil seals. Two development efforts currently being managed by NAWCAD are the High Performance Compressor Discharge Film-Riding Face Seal and the Subsonic Core High Speed Air/Oil Seal. The High Performance Compressor Discharge Film-Riding Face Seal Program aims at reducing parasitic leakage through application of a film-riding face sea concept to the compressor discharge location of a Phase 2 IHPTET engine. An order-of-magnitude leakage reduction relative to current labyrinth seal configurations is expected. Performance goals for these seals are (1) 1200 F air temperature, (2) 800 feet-per-second surface velocity, and (3) 600 SPI differential pressure. The two designs chosen for fabrication and rig test are a spiral groove and a Rayleigh step seal. Rig testing is currently underway. The Subsonic Core High Speed Air/Oil Seal Program is developing shaft-to-ground seals for next-generation propulsion systems that will minimize leakage and provide full life. Significantly higher rotor speeds and temperatures will be experienced. Technologies being exploited include, hydrodynamic lift assist features, ultra light weight designs, and improved cooling schemes. Parametric testing has been completed; a final seal design is entering the endurance test phase.

  8. Navy GTE seal development activity

    NASA Astrophysics Data System (ADS)

    Grala, Carl P.

    1993-10-01

    Under the auspices of the Integrated High Performance Turbine Engine Technology Initiative, the Naval Air Warfare Center conducts advanced development programs for demonstration in the next generation of air-breathing propulsion systems. Among the target technologies are gas path and lube oil seals. Two development efforts currently being managed by NAWCAD are the High Performance Compressor Discharge Film-Riding Face Seal and the Subsonic Core High Speed Air/Oil Seal. The High Performance Compressor Discharge Film-Riding Face Seal Program aims at reducing parasitic leakage through application of a film-riding face sea concept to the compressor discharge location of a Phase 2 IHPTET engine. An order-of-magnitude leakage reduction relative to current labyrinth seal configurations is expected. Performance goals for these seals are (1) 1200 F air temperature, (2) 800 feet-per-second surface velocity, and (3) 600 SPI differential pressure. The two designs chosen for fabrication and rig test are a spiral groove and a Rayleigh step seal. Rig testing is currently underway. The Subsonic Core High Speed Air/Oil Seal Program is developing shaft-to-ground seals for next-generation propulsion systems that will minimize leakage and provide full life. Significantly higher rotor speeds and temperatures will be experienced. Technologies being exploited include, hydrodynamic lift assist features, ultra light weight designs, and improved cooling schemes. Parametric testing has been completed; a final seal design is entering the endurance test phase.

  9. Closure and Sealing Design Calculation

    SciTech Connect

    T. Lahnalampi; J. Case

    2005-08-26

    The purpose of the ''Closure and Sealing Design Calculation'' is to illustrate closure and sealing methods for sealing shafts, ramps, and identify boreholes that require sealing in order to limit the potential of water infiltration. In addition, this calculation will provide a description of the magma that can reduce the consequences of an igneous event intersecting the repository. This calculation will also include a listing of the project requirements related to closure and sealing. The scope of this calculation is to: summarize applicable project requirements and codes relating to backfilling nonemplacement openings, removal of uncommitted materials from the subsurface, installation of drip shields, and erecting monuments; compile an inventory of boreholes that are found in the area of the subsurface repository; describe the magma bulkhead feature and location; and include figures for the proposed shaft and ramp seals. The objective of this calculation is to: categorize the boreholes for sealing by depth and proximity to the subsurface repository; develop drawing figures which show the location and geometry for the magma bulkhead; include the shaft seal figures and a proposed construction sequence; and include the ramp seal figure and a proposed construction sequence. The intent of this closure and sealing calculation is to support the License Application by providing a description of the closure and sealing methods for the Safety Analysis Report. The closure and sealing calculation will also provide input for Post Closure Activities by describing the location of the magma bulkhead. This calculation is limited to describing the final configuration of the sealing and backfill systems for the underground area. The methods and procedures used to place the backfill and remove uncommitted materials (such as concrete) from the repository and detailed design of the magma bulkhead will be the subject of separate analyses or calculations. Post-closure monitoring will not

  10. TECHNOLOGY ROADMAPPING FOR IAEA SEALS.

    SciTech Connect

    HOFFHEINS,B.; ANNESE,C.; GOODMAN,M.; OCONNOR,W.; GUSHUE,S.; PEPPER,S.

    2003-07-13

    In the fall of 2002, the U.S. Support Program (USSP) initiated an effort to define a strategy or ''roadmap'' for future seals technologies and to develop a generalized process for planning safeguards equipment development, which includes seals and other safeguards equipment. The underlying objectives of the USSP include becoming more proactive than reactive in addressing safeguards equipment needs, helping the IAEA to maintain an inventory of cost-effective, reliable, and effective safeguards equipment, establishing a long-term planning horizon, and securing IAEA ownership in the process of effective requirements definition and timely transitioning of new or improved systems for IAEA use. At an initial workshop, seals, their functions, performance issues, and future embodiments were discussed in the following order: adhesive seals, metal seals, passive and active loop seals, ultrasonic seals, tamper indicating enclosures (including sample containers, equipment enclosures, and conduits). Suggested improvements to these technologies focused largely on a few themes: (1) The seals must be applied quickly, easily, and correctly; (2) Seals and their associated equipment should not unduly add bulk or weight to the inspectors load; (3) Rapid, in-situ verifiability of seals is desirable; and (4) Seal systems for high risk or high value applications should have two-way, remote communications. Based upon these observations and other insights, the participants constructed a skeletal approach for seals technology planning. The process begins with a top-level review of the fundamental safeguards requirements and extraction of required system features, which is followed by analysis of suitable technologies and identification of technology gaps, and finally by development of a planning schedule for system improvements and new technology integration. Development of a comprehensive procedure will require the partnership and participation of the IAEA. The presentation will include a

  11. Pneumatic stowing seals mines

    SciTech Connect

    Brezovec, D.

    1983-11-01

    A mechanized technique to seal abandoned mines has been used successfully to close 13 openings at Duquesne Light Co.'s mined-out Warwick No. 2 mine, near Greensboro, Pa. The mechanized system, which uses a pneumatic stower and crushed limestone, closed the entries more economically and in less time than it would have taken to install traditional concrete block stopping and clay plug seals, according to John C. Draper. Draper, a mining engineer with Duquesne Light's coal department, was in charge of installing the Warwick seals in a Bureau of Mines-sponsored field test on the pneumatic sealing technique. The lowest estimated cost for installing conventional stopping and plug closures for the 13 Warwick openings was $225,000, says Draper, while the openings were closed using the mechanized system for $245,000. Draper says the newer stopping cost more in the instance because work was stopped often to gather information for the experiment. The experimental closures were installed in 38 days. The job would have taken at least 149 days if traditional closures were being installed, Draper say. To install a traditional concrete block/clay plug closure, the mine opening must be cleaned thoroughly and the roof must be supported for some 3 ft from the outside. Then a solid wall or stopping must be built 25 ft from the surface and the entry must be packed with clay to the surface. Much of this job requires workers to remain underground. In pneumatic stowing, 1 1/2-in. crushed limestone with fines is conveyed through a pipeline and into the mine opening under low air pressure. Watertight seals can be installed by blowing about 10 ft of rock into the opening against the top to act as roof support. Safety posts are installed and about 10 or 15 ft of mine entry is cleaned. About 2 in. of raw cement or bentonite is placed on the floor and limestone mixed with dry cement or bentonite is blown into the opening.

  12. Canister, Sealing Method And Composition For Sealing A Borehole

    SciTech Connect

    Brown, Donald W.; Wagh, Arun S.

    2005-06-28

    Method and composition for sealing a borehole. A chemically bonded phosphate ceramic sealant for sealing, stabilizing, or plugging boreholes is prepared by combining an oxide or hydroxide and a phosphate with water to form slurry. The slurry is introduced into the borehole where the seal, stabilization or plug is desired, and then allowed to set up to form the high strength, minimally porous sealant, which binds strongly to itself and to underground formations, steel and ceramics.

  13. QwikSeal (registered trademark) Pre-Sealed Aerospace Fasteners

    DTIC Science & Technology

    2012-08-29

    UV - curable and solvent-based systems being evaluated – New application methodology being integrated onto QwikSeal production machine Automated...materials with a wide range of viscosities – Solvent based and 100% solids UV - curable materials can both be sprayed with fine control • Bench top...Std Z39-18 Overview 2 Background Sealant Barrier Coat PCI ESTCP Program Integration • QwikSeal Background – Problem – QwikSeal Solution

  14. Some comparison of ECMWF 3b and GFDL 3b analyses in the tropics

    NASA Technical Reports Server (NTRS)

    Julian, P. R.

    1985-01-01

    Owing to the problems that data assimilation procedures have in analyzing data in the tropics and to the apparent differences in the descriptions of the European Center for Medium Range Weather Forecasts (ECMWF) and Geophysical Fluid Dynamics Laboratory (GFDL) assimilation suites, a brief comparison was done of the resulting analyses in the tropics. Three cases each were selected for Standard Operating Procedures 1 and 2 at the 200 and 850 mb levels, the wind fields for 25 deg N 25 deg S were partitioned into a rotational and divergent component, and various statistics of each were calculated.

  15. High temperature braided rope seals for static sealing applications

    NASA Technical Reports Server (NTRS)

    Adams, Michael L.; Olsen, Andrew; Darolia, Ram; Steinetz, Bruce M.; Bartolotta, Paul A.

    1996-01-01

    Achieving efficiency and performance goals of advanced aircraft and industrial systems are leading designers to implement high temperature materials such as ceramics and intermetallics. Generally these advanced materials are applied selectively in the highest temperature sections of the engine system including the combustor and high pressure turbine, amongst others. Thermal strains that result in attaching the low expansion-rate components to high expansion rate superalloy structures can cause significant life reduction in the components. Seals are being designed to both seal and to serve as compliant mounts allowing for relative thermal growths between high temperature but brittle primary structures and the surrounding support structures. Designers require high temperature, low-leakage, compliant seals to mitigate thermal stresses and control parasitic and cooling airflow between structures. NASA is developing high temperature braided rope seals in a variety of configurations to help solve these problems. This paper will describe the types of seals being developed, describe unique test techniques used to assess seal performance, and present leakage flow data under representative pressure, temperature and scrubbing conditions. Feasibility of the braided rope seals for both an industrial tube seal and a turbine vane seal application is also demonstrated.

  16. Setting properties and sealing ability of hydraulic temporary sealing materials.

    PubMed

    Ogura, Yoko; Katsuumi, Ichiroh

    2008-09-01

    This study sought to investigate the setting progress and sealing ability of hydraulic temporary sealing materials used in endodontic treatment: Lumicon, Caviton, and HY-Seal. To evaluate setting progress, the materials were filled into glass tubes with one end sealed and immersed in water. After immersion, a measurement apparatus was inserted from the non-immersed end and the set area was determined by subtracting the unset area from the sample thickness. To evaluate sealing ability, materials were filled into glass tubes and divided into four groups based on different immersion times. Thermal cycling and dye penetration were performed. At 7 days, the setting depths of HY-Seal and Caviton were almost equivalent to full sample thickness, while that of Lumicon was only half of full sample thickness (p < 0.01). On sealing ability, Lumicon ranked the highest followed by Caviton, whereas HY-Seal was unstable (p < 0.01). These results suggested that there was no correlation between setting progress and sealing ability.

  17. Seals cap rotary kiln emissions

    SciTech Connect

    Gunkle, D.W. )

    1993-09-01

    The possibility of producing fugitive emissions is one of the most critical aspects of an incineration system. Whether such a system processes hazardous, medical, mixed or municipal waste, fugitive emissions are of special concern to system operators and the public alike. Effectively designed rotary-kiln seals can reduce fugitive emissions to acceptable, minimal levels. Modern air monitoring systems track incineration site emissions. Possible emissions sources include excavation and transfer sites, storage areas, material-feed systems, rotary kiln seals, and exhaust stacks. Several options are available for rotary-kiln seals. Six are discussed here: labyrinth; overlapping spring plate; graphite block; pneumatic; shrouded; and overpressure. Kiln seals are used to prevent process gases from escaping or ambient air from entering a rotary kiln uncontrolled. They are not designed to function as material seals, or prevent spills of solids or liquids. Seal design involves considering differential pressure produced by a kiln's internal-to-external temperature, pressure excursions (explosions) and material spills.

  18. Metal to ceramic sealed joint

    DOEpatents

    Lasecki, J.V.; Novak, R.F.; McBride, J.R.

    1991-08-27

    A metal to ceramic sealed joint which can withstand wide variations in temperature and maintain a good seal is provided for use in a device adapted to withstand thermal cycling from about 20 to about 1000 degrees C. The sealed joint includes a metal member, a ceramic member having an end portion, and an active metal braze forming a joint to seal the metal member to the ceramic member. The joint is positioned remote from the end portion of the ceramic member to avoid stresses at the ends or edges of the ceramic member. The sealed joint is particularly suited for use to form sealed metal to ceramic joints in a thermoelectric generator such as a sodium heat engine where a solid ceramic electrolyte is joined to metal parts in the system. 11 figures.

  19. Metal to ceramic sealed joint

    DOEpatents

    Lasecki, John V.; Novak, Robert F.; McBride, James R.

    1991-01-01

    A metal to ceramic sealed joint which can withstand wide variations in temperature and maintain a good seal is provided for use in a device adapted to withstand thermal cycling from about 20 to about 1000 degrees C. The sealed joint includes a metal member, a ceramic member having an end portion, and an active metal braze forming a joint to seal the metal member to the ceramic member. The joint is positioned remote from the end portion of the ceramic member to avoid stresses at the ends or edges of the ceramic member. The sealed joint is particularly suited for use to form sealed metal to ceramic joints in a thermoelectric generator such as a sodium heat engine where a solid ceramic electrolyte is joined to metal parts in the system.

  20. Aft outer rim seal arrangement

    SciTech Connect

    Lee, Ching-Pang; Tham, Kok-Mun; Schroeder, Eric; Meeroff, Jamie; Miller, Jr., Samuel R; Marra, John J; Campbell, Christian X

    2015-04-28

    An outer rim seal arrangement (10), including: an annular rim (70) centered about a longitudinal axis (30) of a rotor disc (31), extending fore and having a fore-end (72), an outward-facing surface (74), and an inward-facing surface (76); a lower angel wing (62) extending aft from a base of a turbine blade (22) and having an aft end (64) disposed radially inward of the rim inward-facing surface to define a lower angel wing seal gap (80); an upper angel wing (66) extending aft from the turbine blade base and having an aft end (68) disposed radially outward of the rim outward-facing surface to define a upper angel wing seal gap (80, 82); and guide vanes (100) disposed on the rim inward-facing surface in the lower angel wing seal gap. Pumping fins (102) may be disposed on the upper angel wing seal aft end in the upper angel wing seal gap.

  1. Maintenance of DNA methylation: Dnmt3b joins the dance.

    PubMed

    Walton, Emma L; Francastel, Claire; Velasco, Guillaume

    2011-11-01

    DNA methylation mostly occurs within the context of CpG dinucleotides and is essential for embryonic development and gene repression. It is generally accepted that DNA methyltransferases carry out specific and non-overlapping functions, Dnmt3a and Dnmt3b being responsible for the establishment of methylation around the time of implantation and Dnmt1 ensuring that methylation is faithfully copied to daughter cells via what has come to be known as "maintenance methylation." This longstanding view has been challenged over the years with the observation that Dnmt1 alone is incapable of perfect maintenance methylation. A new model is emerging that takes into account a contribution of the de novo enzymes Dnmt3a and Dnmt3b in the maintenance of the DNA methylation. We recently showed that certain germ line genes are specific targets of Dnmt3b, and that Dnmt3b remains bound to their promoter regions in somatic cells via interaction with the transcriptional repressor E2F6. It is tempting to consider an ongoing role for Dnmt3b in the methylation of germ line genes in somatic cells. We propose here observations in support of the hypothesis that the maintenance of methylation and subsequent silencing of a handful of germ line genes requires Dnmt3b but not Dnmt1. In addition to suggesting a new role for Dnmt3b in the protection of somatic cells against the promiscuous expression of the germ line program, these observations are of particular interest in the field of carcinogenesis, given that the expression of catalytically inactive Dnmt3b isoforms and aberrant expression of germ line genes are commonly observed in cancer cells.

  2. Rim seal for turbine wheel

    DOEpatents

    Glezer, Boris; Boyd, Gary L.; Norton, Paul F.

    1996-01-01

    A turbine wheel assembly includes a disk having a plurality of blades therearound. A ceramic ring is mounted to the housing of the turbine wheel assembly. A labyrinth rim seal mounted on the disk cooperates with the ceramic ring to seal the hot gases acting on the blades from the disk. The ceramic ring permits a tighter clearance between the labyrinth rim seal and the ceramic ring.

  3. Immunogenetics of the Elephant Seal

    NASA Technical Reports Server (NTRS)

    Garza, John Carlos

    1999-01-01

    The goals of this cooperative agreement fall into three categories: 1) A basic description of Immunogenetic variation in the northern elephant seal genome; 2) A basic genetic map of the northern elephant seal genome; 3). Microevolutionary forces in the northern elephant seal genome. The results described in this report were acquired using funds from this cooperative agreement together with funds from a National Science Foundation Dissertation Improvement Grant.

  4. Low-Torque Seal Development

    NASA Technical Reports Server (NTRS)

    Lattime, Scott B.; Borowski, Richard

    2009-01-01

    The EcoTurn Class K production prototypes have passed all AAR qualification tests and received conditional approval. The accelerated life test on the second set of seals is in progress. Due to the performance of the first set, no problems are expected.The seal has demonstrated superior performance over the HDL seal in the test lab with virtually zero torque and excellent contamination exclusion and grease retention.

  5. 30 CFR 57.8535 - Seals.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ....8535 Seals. Seals shall be provided with a means for checking the quality of air behind the seal and a means to prevent a water head from developing unless the seal is designed to impound water. ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Seals. 57.8535 Section 57.8535...

  6. 19 CFR 113.25 - Seals.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Seals. 113.25 Section 113.25 Customs Duties U.S... Bond Requirements § 113.25 Seals. When a seal is required, the seal shall be affixed adjoining the signatures of principal and surety, if individuals, and the corporate seal shall be affixed close to...

  7. 19 CFR 113.25 - Seals.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 1 2013-04-01 2013-04-01 false Seals. 113.25 Section 113.25 Customs Duties U.S... Bond Requirements § 113.25 Seals. When a seal is required, the seal shall be affixed adjoining the signatures of principal and surety, if individuals, and the corporate seal shall be affixed close to...

  8. 19 CFR 113.25 - Seals.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 1 2012-04-01 2012-04-01 false Seals. 113.25 Section 113.25 Customs Duties U.S... Bond Requirements § 113.25 Seals. When a seal is required, the seal shall be affixed adjoining the signatures of principal and surety, if individuals, and the corporate seal shall be affixed close to...

  9. 19 CFR 113.25 - Seals.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 1 2014-04-01 2014-04-01 false Seals. 113.25 Section 113.25 Customs Duties U.S... Bond Requirements § 113.25 Seals. When a seal is required, the seal shall be affixed adjoining the signatures of principal and surety, if individuals, and the corporate seal shall be affixed close to...

  10. 19 CFR 113.25 - Seals.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 1 2011-04-01 2011-04-01 false Seals. 113.25 Section 113.25 Customs Duties U.S... Bond Requirements § 113.25 Seals. When a seal is required, the seal shall be affixed adjoining the signatures of principal and surety, if individuals, and the corporate seal shall be affixed close to...

  11. Borehole sealing method and apparatus

    DOEpatents

    Hartley, James N.; Jansen, Jr., George

    1977-01-01

    A method and apparatus is described for sealing boreholes in the earth. The borehole is blocked at the sealing level, and a sealing apparatus capable of melting rock and earth is positioned in the borehole just above seal level. The apparatus is heated to rock-melting temperature and powdered rock or other sealing material is transported down the borehole to the apparatus where it is melted, pooling on the mechanical block and allowed to cool and solidify, sealing the hole. Any length of the borehole can be sealed by slowly raising the apparatus in the borehole while continuously supplying powdered rock to the apparatus to be melted and added to the top of the column of molten and cooling rock, forming a continuous borehole seal. The sealing apparatus consists of a heater capable of melting rock, including means for supplying power to the heater, means for transporting powdered rock down the borehole to the heater, means for cooling the apparatus and means for positioning the apparatus in the borehole.

  12. Electrodes for sealed secondary batteries

    NASA Technical Reports Server (NTRS)

    Boies, D. B.; Child, F. T.

    1972-01-01

    Self-supporting membrane electrode structures, in which active ingredients and graphite are incorporated in a polymeric matrix, improve performance of electrodes in miniature, sealed, alkaline storage batteries.

  13. Internal coaxial cable seal system

    DOEpatents

    Hall, David R.; Sneddon, Cameron; Dahlgren, Scott Steven; Briscoe, Michael A.

    2006-07-25

    The invention is a seal system for a coaxial cable and is placed within the coaxial cable and its constituent components. A series of seal stacks including load ring components and elastomeric rings are placed on load bearing members within the coaxial cable sealing the annular space between the coaxial cable and an electrical contact passing there through. The coaxial cable is disposed within drilling components to transmit electrical signals between drilling components within a drill string. The seal system can be used in a variety of downhole components, such as sections of pipe in a drill string, drill collars, heavy weight drill pipe, and jars.

  14. Pressure sensor for sealed containers

    DOEpatents

    Hodges, Franklin R.

    2001-01-01

    A magnetic pressure sensor for sensing a pressure change inside a sealed container. The sensor includes a sealed deformable vessel having a first end attachable to an interior surface of the sealed container, and a second end. A magnet mounted to the vessel second end defining a distance away from the container surface provides an externally detectable magnetic field. A pressure change inside the sealed container causes deformation of the vessel changing the distance of the magnet away from the container surface, and thus the detectable intensity of the magnetic field.

  15. Advanced High Temperature Structural Seals

    NASA Technical Reports Server (NTRS)

    Newquist, Charles W.; Verzemnieks, Juris; Keller, Peter C.; Rorabaugh, Michael; Shorey, Mark

    2002-01-01

    This program addresses the development of high temperature structural seals for control surfaces for a new generation of small reusable launch vehicles. Successful development will contribute significantly to the mission goal of reducing launch cost for small, 200 to 300 pound payloads. Development of high temperature seals is mission enabling. For instance, ineffective control surface seals can result in high temperature (3100 F) flows in the elevon area exceeding structural material limits. Longer sealing life will allow use for many missions before replacement, contributing to the reduction of hardware, operation and launch costs.

  16. SEAL FOR HIGH SPEED CENTRIFUGE

    DOEpatents

    Skarstrom, C.W.

    1957-12-17

    A seal is described for a high speed centrifuge wherein the centrifugal force of rotation acts on the gasket to form a tight seal. The cylindrical rotating bowl of the centrifuge contains a closure member resting on a shoulder in the bowl wall having a lower surface containing bands of gasket material, parallel and adjacent to the cylinder wall. As the centrifuge speed increases, centrifugal force acts on the bands of gasket material forcing them in to a sealing contact against the cylinder wall. This arrangememt forms a simple and effective seal for high speed centrifuges, replacing more costly methods such as welding a closure in place.

  17. High Temperature Hydraulic Seals

    DTIC Science & Technology

    1993-05-01

    test rods were made from PH13 - 8 Mo corrosion resistant steel, hardened to Rc=45. Two unsuccessful attempts were made to test the rod seals. In both...BARRELS MATERIAL AND HEAT TREAT NOTES: 1. Material shall be 2-3/ 8 inch diameter PH13 -8Mo stainless steel bars per AMS 5629, purchased in condition "A...s PE-62203 PR-3145 6. AUTHOR(S) TA- 30 K. R. Williams WU-45 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8 . PERFORMING ORGANIZATION REPORT

  18. Mechanical Face Seal Dynamics.

    DTIC Science & Technology

    1985-12-01

    Fig. 4(a)]. The axial runout at the tuter radius 6- TABLE 1: PARAMWtIER VALUlFS FOiR THE. SINtGt.t PER UtLRimAlTIN Scin.i’ IIN SCIIEI;Mt Mass, m,n kg...critical rotor runout are presented. It is shown that the more simple to use small perturbation analysis gives very good results for most practical...stiffness and damping coefficients i : are not constants. In the case of seal-rotor runout , the stiffness and damping coefficients of the elastomer are

  19. DNA cytosine and methylcytosine deamination by APOBEC3B: enhancing methylcytosine deamination by engineering APOBEC3B.

    PubMed

    Fu, Yang; Ito, Fumiaki; Zhang, Gewen; Fernandez, Braulio; Yang, Hanjing; Chen, Xiaojiang S

    2015-10-01

    APOBEC (apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like) is a family of enzymes that deaminates cytosine (C) to uracil (U) on nucleic acid. APOBEC3B (A3B) functions in innate immunity against intrinsic and invading retroelements and viruses. A3B can also induce genomic DNA mutations to cause cancer. A3B contains two cytosine deaminase domains (CD1, CD2), and there are conflicting reports about whether both domains are active. Here we demonstrate that only CD2 of A3B (A3BCD2) has C deamination activity. We also reveal that both A3B and A3BCD2 can deaminate methylcytosine (mC). Guided by structural and functional analysis, we successfully engineered A3BCD2 to gain over two orders of magnitude higher activity for mC deamination. Important determinants that contribute to the activity and selectivity for mC deamination have been identified, which reveals that multiple elements, rather than single ones, contribute to the mC deamination activity and selectivity in A3BCD2 and possibly other APOBECs.

  20. Reconsolidation of salt as applied to permanent seals for the Waste Isolation Pilot Plant

    SciTech Connect

    Hansen, F.D.; Callahan, G.D.; Van Sembeek, L.L.

    1993-07-01

    Reconsolidated salt is a fundamental component of the permanent seals for the Waste Isolation Pilot Plant. As regulations are currently understood and seal concepts envisioned, emplaced salt is the sole long-term seal component designed to prevent the shafts from becoming preferred pathways for rating gases or liquids. Studies under way in support of the sealing function of emplaced salt include laboratory testing of crushed salt small-scale in situ tests, constitutive modeling of crushed salt, calculations of the opening responses during operation and closure, and design practicalities including emplacement techniques. This paper briefly summarizes aspects of these efforts and key areas of future work.

  1. Disperser seal and method

    SciTech Connect

    Johnston, R. T.

    1981-06-02

    A seal is described for a shaft of a disperser crusher, that pulverizes hot coal particles, maintains a higher than atmospheric pressure within a casing for the crusher, and is able to withstand elevated temperatures that are produced within the casing. The pressure and temperature result from hot gases that convey coal particles to the crusher. The seal includes self lubricating graphite packings that are urged in abutting relation with a smooth, ceramic sleeve on the shaft and are able to withstand the temperature on the shaft surface. A first, interior packing is on the inside of a wall of the casing while a second, exterior packing is outside of the wall. Superheated steam, a gas inert with the coal particles, is supplied to the interior packing with sufficient pressure to substantially prevent the migration of coal particles through the interior packing. The tendency of the coal particles to migrate from the container through the interior packing is further inhibited by providing a tortuous path from the casing to the interior packing.

  2. Management of disused plutonium sealed sources

    SciTech Connect

    Whitworth, Julia Rose; Pearson, Michael W; Abeyta, Cristy

    2010-01-01

    The Global Threat Reduction Initiative's (GTRI) Offsite Source Recovery Project (OSRP) has been recovering excess and unwanted radioactive sealed sources since 1999, including more than 2,400 Plutonium (Pu)-238 sealed sources and 653 Pu-239-bearing sources that represent more than 10% of the total sources recovered by GTRI/OSRP to date. These sources have been recovered from hundreds of sites within the United States (US) and around the world. OSRP grew out of early efforts at the Los Alamos National Laboratory (LANL) to recover and disposition excess Plutonium-239 (Pu-239) sealed sources that were distributed in the 1960s and 1970s under the Atoms for Peace Program, a loan-lease program that serviced 31 countries, as well as domestic users. In the conduct of these recovery operations, GTRI/OSRP has been required to solve problems related to knowledge-of-inventory, packaging and transportation of fissile and heat-source materials, transfer of ownership, storage of special nuclear material (SNM) both at US Department of Energy (DOE) facilities and commercially, and disposal. Unique issues associated with repatriation from foreign countries, including end user agreements required by some European countries and denials of shipment, will also be discussed.

  3. Qualification tests for 192Ir sealed sources

    NASA Astrophysics Data System (ADS)

    Iancso, Georgeta; Iliescu, Elena; Iancu, Rodica

    2013-12-01

    This paper describes the results of qualification tests for 192Ir sealed sources, available in Testing and Nuclear Expertise Laboratory of National Institute for Physics and Nuclear Engineering "Horia Hulubei" (I.F.I.N.-HH), Romania. These sources had to be produced in I.F.I.N.-HH and were tested in order to obtain the authorization from The National Commission for Nuclear Activities Control (CNCAN). The sources are used for gammagraphy procedures or in gammadefectoscopy equipments. Tests, measurement methods and equipments used, comply with CNCAN, AIEA and International Quality Standards and regulations. The qualification tests are: 1. Radiological tests and measurements: dose equivalent rate at 1 m; tightness; dose equivalent rate at the surface of the transport and storage container; external unfixed contamination of the container surface. 2. Mechanical and climatic tests: thermal shock; external pressure; mechanic shock; vibrations; boring; thermal conditions for storage and transportation. Passing all tests, it was obtained the Radiological Security Authorization for producing the 192Ir sealed sources. Now IFIN-HH can meet many demands for this sealed sources, as the only manufacturer in Romania.

  4. Seal between metal and ceramic conduits

    DOEpatents

    Underwood, Richard Paul; Tentarelli, Stephen Clyde

    2015-02-03

    A seal between a ceramic conduit and a metal conduit of an ion transport membrane device consisting of a sealing surface of ceramic conduit, a sealing surface of ceramic conduit, a single gasket body, and a single compliant interlayer.

  5. Toward an Improved Hypersonic Engine Seal

    NASA Technical Reports Server (NTRS)

    Dunlap, Patrick H., Jr.; Steinetz, Bruce M.; DeMange,Jeffrey J.; Taylor, Shawn C.

    2003-01-01

    High temperature, dynamic seals are required in advanced engines to seal the perimeters of movable engine ramps for efficient, safe operation in high heat flux environments at temperatures from 2000 to 2500 F. Current seal designs do not meet the demanding requirements for future engines, so NASA s Glenn Research Center (GRC) is developing advanced seals to overcome these shortfalls. Two seal designs and two types of seal preloading devices were evaluated in a series of compression tests at room temperature and 2000 F and flow tests at room temperature. Both seals lost resiliency with repeated load cycling at room temperature and 2000 F, but seals with braided cores were significantly more flexible than those with cores composed of uniaxial ceramic fibers. Flow rates for the seals with cores of uniaxial fibers were lower than those for the seals with braided cores. Canted coil springs and silicon nitride compression springs showed promise conceptually as potential seal preloading devices to help maintain seal resiliency.

  6. Ceramic-to-metal vacuum seal

    NASA Technical Reports Server (NTRS)

    Sackerlotzky, O. H.

    1979-01-01

    Knife-edge sealing technique forms reliable, vacuum-tight bonds between materials having very different thermal-expansion characteristics. Seal is thin and flexible and absorb shear, hoop, and bonding stresses at joint so that seal remains vacuum tight.

  7. Compliant high temperature seals for dissimilar materials

    DOEpatents

    Rynders, Steven Walton; Minford, Eric; Tressler, Richard Ernest; Taylor, Dale M.

    2001-01-01

    A high temperature, gas-tight seal is formed by utilizing one or more compliant metallic toroidal ring sealing elements, where the applied pressure serves to activate the seal, thus improving the quality of the seal. The compliant nature of the sealing element compensates for differences in thermal expansion between the materials to be sealed, and is particularly useful in sealing a metallic member and a ceramic tube art elevated temperatures. The performance of the seal may be improved by coating the sealing element with a soft or flowable coating such as silver or gold and/or by backing the sealing element with a bed of fine powder. The material of the sealing element is chosen such that the element responds to stress elastically, even at elevated temperatures, permitting the seal to operate through multiple thermal cycles.

  8. Sealed Plant-Growth Chamber For Clinostat

    NASA Technical Reports Server (NTRS)

    Brown, Christopher S.; Dreschel, Thomas W.

    1993-01-01

    Laboratory chamber for growing plants used to measure photosynthesis and respiration in simulated microgravity. Holds plant specimens while rotated on clinostat, see article, "Clinostat Delivers Power To Plant-Growth Cabinets" (KSC-11537). Provides way of comparing gas-exchange rates of plants rotated horizontally on clinostat with those of stationary or vertically rotated plants. Gas extracted for analysis without stopping clinostat. Chamber includes potlike base and cylindrical cover, both made of transparent acrylic pipe. Gasket forms seal between cover and bottom plate of base. Cover bolted to pot baseplate, which in turn bolted to clinostat.

  9. Face-Sealing Butterfly Valve

    NASA Technical Reports Server (NTRS)

    Tervo, John N.

    1992-01-01

    Valve plate made to translate as well as rotate. Valve opened and closed by turning shaft and lever. Interactions among lever, spring, valve plate, and face seal cause plate to undergo combination of translation and rotation so valve plate clears seal during parts of opening and closing motions.

  10. Liquid trap seals thermocouple leads

    NASA Technical Reports Server (NTRS)

    Ruppe, E. P.

    1966-01-01

    Liquid trap seals thermocouple leads coming out of a brazing retort that operates with a controlled atmosphere so that air cannot enter the retort and hydrogen cannot escape. The trap is fastened to a duct welded to the retort. Thermocouple leads are led out through the duct and trap, with the fluid forming a gastight seal between the atmosphere and the retort.

  11. Rotating plug bearing and seal

    DOEpatents

    Wade, Elman E.

    1977-01-01

    A bearing and seal structure for nuclear reactors utilizing rotating plugs above the nuclear reactor vessel. The structure permits lubrication of bearings and seals of the rotating plugs without risk of the lubricant draining into the reactor vessel below. The structure permits lubrication by utilizing a rotating outer race bearing.

  12. Conical O-ring seal

    DOEpatents

    Chalfant, Jr., Gordon G.

    1984-01-01

    A shipping container for radioactive or other hazardous materials which has a conical-shaped closure containing grooves in the conical surface thereof and an O-ring seal incorporated in each of such grooves. The closure and seal provide a much stronger, tighter and compact containment than with a conventional flanged joint.

  13. Boltless Seal for Electronic Housings

    NASA Technical Reports Server (NTRS)

    Dawe, R. H.; Evans, J. T.

    1982-01-01

    Spring clips seal housings for electronic circuitry, preventing electromagnetic interference from entering or leaving housings. Clips also keep dust out of housing. Since no bolts are used, housing can be opened quickly; unlike bolts, clips can be used on thin-walled housing. Seal was developed for an X-band array amplifier.

  14. Conical O-ring seal

    DOEpatents

    Chalfant, G.G. Jr.

    A shipping container for radioactive or other hazardous materials has a conical-shaped closure containing grooves in the conical surface thereof and an O-ring seal incorporated in each of such grooves. The closure and seal provide a much stronger, tighter and compact containment than with a conventional flanged joint.

  15. Seals. Grades 3-6.

    ERIC Educational Resources Information Center

    New England Aquarium, Boston, MA.

    Explanations of a marine mammal rescue program and information on seals and sea lions are presented in this curriculum package for intermediate grade teachers. Activities are highlighted which focus on the natural history of harbor seals. This unit contains: (1) pre-trip activities (including fact sheets and worksheets on the different types of…

  16. Repository Closure and Sealing Approach

    SciTech Connect

    A.T. Watkins

    2000-06-28

    The scope of this analysis will be to develop the conceptual design of the closure seals and their locations in the Subsurface Facilities. The design will be based on the recently established program requirements for transitioning to the Site Recommendation (SR) design as outlined by ''Approach to Implementing the Site Recommendation Baseline'' (Stroupe 2000) and the ''Monitored Geologic Repository Project Description Document'' (CRWMS M&O 1999b). The objective of this analysis will be to assist in providing a description for the Subsurface Facilities System Description Document, Section 2 and finally to document any conclusions reached in order to contribute and provide support to the SR. This analysis is at a conceptual level and is considered adequate to support the SR design. The final closure barriers and seals for the ventilation shafts, and the north and south ramps will require these openings to be permanently sealed to limit excessive air and water inflows and prevent human intrusion. The major tasks identified with closure in this analysis are: (1) Developing the overall subsurface seal layout and identifying design and operational interfaces for the Subsurface Facilities. (2) Summarizing the general site conditions and general rock characteristic with respect to seal location and describing the seal selected. (3) Identify seal construction materials, methodology of construction and strategic locations including design of the seal and plugs. (4) Discussing methods to prevent human intrusion.

  17. Airfoil seal system for gas turbine engine

    SciTech Connect

    None, None

    2013-06-25

    A turbine airfoil seal system of a turbine engine having a seal base with a plurality of seal strips extending therefrom for sealing gaps between rotational airfoils and adjacent stationary components. The seal strips may overlap each other and may be generally aligned with each other. The seal strips may flex during operation to further reduce the gap between the rotational airfoils and adjacent stationary components.

  18. Evaluation of T3B fingerprinting for identification of clinical and environmental Sporothrix species.

    PubMed

    Oliveira, Manoel Marques Evangelista; Franco-Duarte, Ricardo; Romeo, Orazio; Pais, Célia; Criseo, Giuseppe; Sampaio, Paula; Zancope-Oliveira, Rosely Maria

    2015-03-01

    In this study, PCR fingerprinting using the universal primer T3B was applied to distinguish among clinical and environmental species of the Sporothrix complex, Sporothrix brasiliensis, S. globosa, S. mexicana, S. pallida, S. luriei and S. schenckii sensu stricto. The T3B fingerprinting generated clearly distinct banding patterns, allowing the correct identification of all 43 clinical and environmental isolates at the species level, what was confirmed by partial calmodulin gene sequence analyses. This technique is reproducible and provides the identification of all species of the Sporothrix complex with sufficient accuracy to be applied in clinical mycology laboratories as well as in epidemiological studies in order to obtain a better understanding of the epidemiology of sporotrichosis.

  19. Experimental Trichinella infection in seals.

    PubMed

    Kapel, C M O; Measures, L; Møller, L N; Forbes, L; Gajadhar, A

    2003-11-01

    The susceptibility of seals to infection with Trichinella nativa and the cold tolerant characteristics of muscle larvae in seal meat were evaluated. Two grey seals, Halichoerus grypus, were inoculated with 5000 (100 larvae/kg) T. nativa larvae and two grey seals with 50000 (1000 larvae/kg). One seal from each dose group and two control seals were killed at 5 and 10 weeks post-inoculation (p.i.). At 5 weeks p.i., infection was established in both low and high dose seals with mean larval densities of 68 and 472 larvae per gram (lpg), respectively, using eight different muscles for analyses. At 10 weeks p.i., mean larval densities were 531 and 2649 lpg, respectively, suggesting an extended persistence of intestinal worms. In seals with high larval density infections, the distribution of larvae in various muscles was uniform, but in one seal with a low larval density infection, predilection sites of larvae included muscle groups with a relative high blood flow, i.e. diaphragm, intercostal and rear flipper muscles. Trichinella-specific antibody levels, as measured by ELISA, increased during the 10 week experimental period. Infected seal muscle was stored at 5, -5 and -18 degrees C for 1, 4 and 8 weeks. Muscle larvae released from stored seal muscle by artificial digestion were inoculated into mice to assess viability and infectivity. Larvae from seal muscle 10 weeks p.i. tolerated -18 degrees C for 8 weeks but larvae from seal muscle 5 weeks p.i. tolerated only 1 week at -18 degrees C, supporting the hypothesis that freeze tolerance increases with the age of the host-parasite tissue complex. The expressed susceptibility to infection, extended production of larvae, antibody response and freeze tolerance of T. nativa in seals are new findings from the first experimental Trichinella infection in any marine mammal and suggest that pinnipeds (phocids, otariiids or walrus) may acquire Trichinella infection by scavenging even small amounts of infected tissue left by hunters or

  20. Molecular characterization of a KIF3B-like kinesin gene in the testis of Octopus tankahkeei (Cephalopoda, Octopus).

    PubMed

    Dang, Ran; Zhu, Jun-Quan; Tan, Fu-Qing; Wang, Wei; Zhou, Hong; Yang, Wan-Xi

    2012-05-01

    KIF3B is known for maintaining and assembling cilia and flagellum. To date, the function of KIF3B and its relationship with KIF3A during spermiogenesis in the cephalopod Octopus tankahkeei remains unknown. In the present study, we characterized a gene encoding a homologue of rat KIF3B in the O. tankahkeei testis and examined its temporal and spatial expression pattern during spermiogenesis. The cDNA of KIF3B was obtained with degenerate and RACE PCR and the distribution pattern of ot-kif3b were observed with RT-PCR. The morphological development during spermiogenesis was illustrated by histological and transmission electron microscopy and mRNA expression of ot-kif3b was observed by in situ hybridization. The 2,365 nucleotides cDNA consisted of a 102 bp 5' untranslated region (UTR), a 2,208 bp open reading frame (ORF) encoding a protein of 736 amino acids, and a 55 bp 3' UTR. Multiple alignments revealed that the putative Ot-KIF3B shared 68, 68, 69, 68, and 67% identity with that of Homo sapiens, Mus musculus, Gallus gallus, Danio rerio, and Xenopus laevis, respectively, along with high identities with Ot-KIF3A in fundamental structures. Ot-kif3b transcripts appeared gradually in early spermatids, increased in intermediate spermatids and maximized in drastically remodeled and final spermatids. The kif3b gene is identified and its expression pattern is demonstrated for the first time in O. tankahkeei. Compared to ot-kif3a reported by our laboratory before, our data suggested that the putative heterodimeric motor proteins Ot-KIF3A/B may be involved in intraspermatic transport and might contribute to structural changes during spermiogenesis.

  1. Crystal structure of inactive form of Rab3B

    SciTech Connect

    Zhang, Wei; Shen, Yang; Jiao, Ronghong; Liu, Yanli; Deng, Lingfu; Qi, Chao

    2012-02-24

    Highlights: Black-Right-Pointing-Pointer This is the first structural information of human Rab3B. Black-Right-Pointing-Pointer To provides a structural basis for the GDP/GTP switch in controlling the activity of Rab3. Black-Right-Pointing-Pointer The charge distribution of Rab3B indicates its unique roles in vesicular trafficking. -- Abstract: Rab proteins are the largest family of ras-related GTPases in eukaryotic cells. They act as directional molecular switches at membrane trafficking, including vesicle budding, cargo sorting, transport, tethering, and fusion. Here, we generated and crystallized the Rab3B:GDP complex. The structure of the complex was solved to 1.9 A resolution and the structural base comparison with other Rab3 members provides a structural basis for the GDP/GTP switch in controlling the activity of small GTPase. The comparison of charge distribution among the members of Rab3 also indicates their different roles in vesicular trafficking.

  2. Metal seal for wellhead apparatus

    SciTech Connect

    Boecker, R.A.

    1987-03-03

    A method is described of effecting a fluid-tight seal between a surface of an oil and/or gas well tubing or casing and a wellhead member, wherein the surface has been unprepared to form the seal, comprising the steps of: positioning the wellhead member circumferentially about a stub of the tubing or casing; positioning a metal-to-metal fluid-tight sealing means of the wellhead member adjacent the unprepared surface of the tubing or casing, the sealing means having at least one metallic projection positioned to extend toward the unprepared surface of the tubing or casing; and forcing the metallic projection into and penetrating the unprepared surface of the tubing or casing to form a seal therebetween.

  3. Sealed head access area enclosure

    DOEpatents

    Golden, Martin P.; Govi, Aldo R.

    1978-01-01

    A liquid-metal-cooled fast breeder power reactor is provided with a sealed head access area enclosure disposed above the reactor vessel head consisting of a plurality of prefabricated structural panels including a center panel removably sealed into position with inflatable seals, and outer panels sealed into position with semipermanent sealant joints. The sealant joints are located in the joint between the edge of the panels and the reactor containment structure and include from bottom to top an inverted U-shaped strip, a lower layer of a room temperature vulcanizing material, a separator strip defining a test space therewithin, and an upper layer of a room temperature vulcanizing material. The test space is tapped by a normally plugged passage extending to the top of the enclosure for testing the seal or introducing a buffer gas thereinto.

  4. Gas-path seal technology

    NASA Technical Reports Server (NTRS)

    Zuk, J.

    1976-01-01

    Improved gas-path seals are needed for better fuel economy, longer performance retention, and lower maintenance, particularly in advanced, high-performance gas turbine engines. Problems encountered in gas-path sealing are described, as well as new blade-tip sealing approaches for high-pressure compressors and turbines. These include a lubricant coating for conventional, porous-metal, rub-strip materials used in compressors. An improved hot-press metal alloy shows promise to increase the operating surface temperatures of high-pressure-turbine, blade-tip seals to 1450 K (2150 F). Three ceramic seal materials are also described that have the potential to allow much higher gas-path surface operating temperatures than are possible with metal systems.

  5. Aerodynamic seals for rotary machine

    DOEpatents

    Bidkar, Rahul Anil; Cirri, Massimiliano; Thatte, Azam Mihir; Williams, John Robert

    2016-02-09

    An aerodynamic seal assembly for a rotary machine includes multiple sealing device segments disposed circumferentially intermediate to a stationary housing and a rotor. Each of the segments includes a shoe plate with a forward-shoe section and an aft-shoe section having multiple labyrinth teeth therebetween facing the rotor. The sealing device segment also includes multiple flexures connected to the shoe plate and to a top interface element, wherein the multiple flexures are configured to allow the high pressure fluid to occupy a forward cavity and the low pressure fluid to occupy an aft cavity. Further, the sealing device segments include a secondary seal attached to the top interface element at one first end and positioned about the flexures and the shoe plate at one second end.

  6. SEALING MEANS FOR RELATIVELY ROTATABLE MEMBERS

    DOEpatents

    Skarstrom, C.S.

    1960-10-25

    A sealing means is offered for maintaining a seal between a pair of relatively rotatable members, panticularly between a rotating shaft and a stationary member surrounding the shaft. The sealing is accomplished by means of a flange extending outward radially on each of a plurality of sealing rings mounted on the rotating member which fit into annular grooves in the stationary member and are held in sealing relation therewith by means of spring rings. In addition, means are provided for passing a sealing gas through the seal sunfaces to prevent accumulation of lubricant and for scavenging any gas which may have leaked from the internal member into the seal area.

  7. Detector production for the R3B Si-tracker

    NASA Astrophysics Data System (ADS)

    Borri, M.; Lemmon, R.; Thornhill, J.; Bate, R.; Chartier, M.; Clague, N.; Herzberg, R.-D.; Labiche, M.; Lindsay, S.; Nolan, P.; Pearce, F.; Powell, W.; Wells, D.

    2016-11-01

    R3B is a fixed target experiment which will study reactions with relativistic radioactive beams at FAIR. Its Si-tracker will surround the target volume and it will detect light charged-particles like protons. The detector technology in use consists of double-sided silicon strip sensors wire bonded to the custom made R3B-ASIC. The tracker allows for a maximum of two outer layers and one inner layer. This paper reports on the production of detectors necessary to build the minimum tracking configuration: one inner layer and one outer layer.

  8. High pressure mechanical seal

    NASA Technical Reports Server (NTRS)

    Babel, Henry W. (Inventor); Anderson, Raymond H. (Inventor)

    1996-01-01

    A relatively impervious mechanical seal is formed between the outer surface of a tube and the inside surface of a mechanical fitting of a high pressure fluid or hydraulic system by applying a very thin soft metal layer onto the outer surface of the hard metal tube and/or inner surface of the hard metal fitting. The thickness of such thin metal layer is independent of the size of the tube and/or fittings. Many metals and alloys of those metals exhibit the requisite softness, including silver, gold, tin, platinum, indium, rhodium and cadmium. Suitably, the coating is about 0.0025 millimeters (0.10 mils) in thickness. After compression, the tube and fitting combination exhibits very low leak rates on the order or 10.sup.-8 cubic centimeters per second or less as measured using the Helium leak test.

  9. High pressure mechanical seal

    NASA Technical Reports Server (NTRS)

    Babel, Henry W. (Inventor); Fuson, Phillip L. (Inventor); Chickles, Colin D. (Inventor); Jones, Cherie A. (Inventor); Anderson, Raymond H. (Inventor)

    1995-01-01

    A relatively impervious mechanical seal is formed between the outer surface of a tube and the inside surface of a mechanical fitting of a high pressure fluid or hydraulic system by applying a very thin soft metal layer onto the outer surface of the hard metal tube and/or inner surface of the hard metal fitting, prior to swaging the fitting onto the tube. The thickness of such thin metal layer is independent of the size of the tube and/or fittings. Many metals and alloys of those metals exhibit the requisite softness, including silver, gold, nickel, tin, platinum, indium, rhodium and cadmium. Suitably, the coating is about 0.0025 millimeters (0.10 mils) in thickness. After swaging, the tube and fitting combination exhibits very low leak rates on the order or 10.sup.-8 cubic centimeters per second or less as meaured using the Helium leak test.

  10. Tamper tape seals

    SciTech Connect

    Wright, B.W.; Undem, H.A.

    1994-07-01

    Tamper tapes are appealing for many applications due to their ease of use and relative robustness. Applications include seals for temporary area denial, protection of sensitive equipment, chain-of-custody audit trails, and inventory control practices. A next generation of adhesive tamper tapes is being developed that combines the best features of commercially available devices with additional state-of-the-art features in tamper indication, tamper-resistance, and counterfeit-resistance. The additional features are based on U.S. Department of Energy (DOE) research and development (R&D) activities that were originally associated with preparations for the Strategic Arms Reduction Treaty (START). New features include rapid-set, chemical-cure adhesive systems that allow user-friendly application and layered levels of counterfeit-resistance based on unique {open_quotes}fingerprint{close_quotes} characteristics that can be accessed as desired.

  11. Identification of the heparin binding site on adeno-associated virus serotype 3B (AAV-3B)

    SciTech Connect

    Lerch, Thomas F.; Chapman, Michael S.

    2012-02-05

    Adeno-associated virus is a promising vector for gene therapy. In the current study, the binding site on AAV serotype 3B for the heparan sulfate proteoglycan (HSPG) receptor has been characterized. X-ray diffraction identified a disaccharide binding site at the most positively charged region on the virus surface. The contributions of basic amino acids at this and other sites were characterized using site-directed mutagenesis. Both heparin and cell binding are correlated to positive charge at the disaccharide binding site, and transduction is significantly decreased in AAV-3B vectors mutated at this site to reduce heparin binding. While the receptor attachment sites of AAV-3B and AAV-2 are both in the general vicinity of the viral spikes, the exact amino acids that participate in electrostatic interactions are distinct. Diversity in the mechanisms of cell attachment by AAV serotypes will be an important consideration for the rational design of improved gene therapy vectors.

  12. Identification of the heparin binding site on adeno-associated virus serotype 3B (AAV-3B)

    SciTech Connect

    Lerch, Thomas F.; Chapman, Michael S.

    2012-05-24

    Adeno-associated virus is a promising vector for gene therapy. In the current study, the binding site on AAV serotype 3B for the heparan sulfate proteoglycan (HSPG) receptor has been characterized. X-ray diffraction identified a disaccharide binding site at the most positively charged region on the virus surface. The contributions of basic amino acids at this and other sites were characterized using site-directed mutagenesis. Both heparin and cell binding are correlated to positive charge at the disaccharide binding site, and transduction is significantly decreased in AAV-3B vectors mutated at this site to reduce heparin binding. While the receptor attachment sites of AAV-3B and AAV-2 are both in the general vicinity of the viral spikes, the exact amino acids that participate in electrostatic interactions are distinct. Diversity in the mechanisms of cell attachment by AAV serotypes will be an important consideration for the rational design of improved gene therapy vectors.

  13. 18 CFR 3b.226 - Accounting of disclosures.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Accounting of... IDENTIFIABLE PERSONAL INFORMATION Rules for Disclosure of Records § 3b.226 Accounting of disclosures. (a) The....225(b) (5) and (7). (b) Each system manager will retain the accounting made under paragraph (a)...

  14. 18 CFR 3b.226 - Accounting of disclosures.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Accounting of... IDENTIFIABLE PERSONAL INFORMATION Rules for Disclosure of Records § 3b.226 Accounting of disclosures. (a) The....225(b) (5) and (7). (b) Each system manager will retain the accounting made under paragraph (a)...

  15. 18 CFR 3b.226 - Accounting of disclosures.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Accounting of... IDENTIFIABLE PERSONAL INFORMATION Rules for Disclosure of Records § 3b.226 Accounting of disclosures. (a) The....225(b) (5) and (7). (b) Each system manager will retain the accounting made under paragraph (a)...

  16. 18 CFR 3b.226 - Accounting of disclosures.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Accounting of... IDENTIFIABLE PERSONAL INFORMATION Rules for Disclosure of Records § 3b.226 Accounting of disclosures. (a) The....225(b) (5) and (7). (b) Each system manager will retain the accounting made under paragraph (a)...

  17. 18 CFR 3b.225 - Written consent for disclosure.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Written consent for... IDENTIFIABLE PERSONAL INFORMATION Rules for Disclosure of Records § 3b.225 Written consent for disclosure. (a... communication to any person, or to any other agency, unless it has the written request by, or the prior...

  18. 18 CFR 3b.225 - Written consent for disclosure.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Written consent for... IDENTIFIABLE PERSONAL INFORMATION Rules for Disclosure of Records § 3b.225 Written consent for disclosure. (a... communication to any person, or to any other agency, unless it has the written request by, or the prior...

  19. 18 CFR 3b.226 - Accounting of disclosures.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Accounting of... IDENTIFIABLE PERSONAL INFORMATION Rules for Disclosure of Records § 3b.226 Accounting of disclosures. (a) The....225(b) (5) and (7). (b) Each system manager will retain the accounting made under paragraph (a)...

  20. The crystal structure of URu3B2

    NASA Astrophysics Data System (ADS)

    Rogl, Peter

    1980-09-01

    The crystal structure of URu3B2 has been determined by single crystal X-ray analysis. URu3B2 crystallizes in the trigonal space group P3bar (C131) with hexagonal lattice a = 1.09531(14), c = 0.59353 (8) nm, Z = 8. Intensity measurements were obtained from a fourcircle diffractometer. The structure was solved by Patterson methods and refined by full matrix least squares calculation. The final R-value, R = ∑ |ΔF|/∑ F0, is 0.052 for an asymetric set of 962 independent reflections (l-F0l > 2 σ (F0)). The crystal structure is a twofold superstructure (distortion-derivative) of the CeCo3B2-type cell (a = 2a', c = 2c' and thus closely related to the CaCu5 type structure. The coordination numbers of U are 2 U + 12 Ru + (6 B) and those of Ru atoms 4 U + 6 Ru + 4 B. The isolated boron atoms have tetrakaidekahedral metal coordination 6 Ru + 3 U; no boron-boron contacts occur. The structural chemistry of (Th, U, RE)Ru3B2 phases is discussed.

  1. 27 CFR 21.36 - Formula No. 3-B.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... (2) Miscellaneous uses: 812.Product development and pilot plant uses (own use only). ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Formula No. 3-B. 21.36 Section 21.36 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU,...

  2. DISCOVER-AQ Aircraft Navigational Data P3B (ICT)

    Atmospheric Science Data Center

    2017-03-31

    ... Project Title:  N/A Platform:  NASA P-3B Spatial Coverage:  (37.84, 39.81), (-77.03, -75.18) ... Data for Atmospheric Composition DISCOVER-AQ - NASA Earth Science Mission DISCOVER-AQ - Program Home Page ...

  3. Do crabeater seals forage cooperatively?

    NASA Astrophysics Data System (ADS)

    Gales, Nicholas J.; Fraser, William R.; Costa, Daniel P.; Southwell, Colin

    2004-08-01

    Crabeater seals are abundant pack-ice predators that feed almost exclusively on krill. They have a circumpolar distribution and are generally sighted hauled out on ice floes alone or in pairs. Here we report our observations of a sighting of 150-200 crabeater seals, which were synchronised in their diving and surfacing behaviour, along with a summary of similar observations from western Antarctica of large groups of crabeater seals in synchronous dive cycles. We report on the low frequency of sightings of such groups during Antarctic pack-ice seal surveys in eastern (Greater) Antarctica. We examine plausible hypotheses to explain these observations, and suggest this behaviour is likely to represent some form of cooperative foraging behaviour, whereby a net advantage in individual energy intake rates is conferred to each seal. Current research on crabeater seal foraging using satellite-linked dive recorders is unlikely to provide sufficiently fine-scale data to examine this hypothesis. Nor will this approach indicate if a seal is foraging with conspecifics. The use of remote or animal-borne camera systems is more likely to provide an insight into fine-scale foraging tactics, as well as the possible, occasional use of cooperative foraging strategies.

  4. Static pressure seal of earmolds.

    PubMed

    Macrae, J

    1990-01-01

    Four experiments were carried out to investigate the effect on the static pressure seal of earmolds made from currently used impression and earmold materials; the occasional practice of making more than one earmold from an impression; the earmoldmaker buildup of impressions; and the multistage buildup of impressions by the impression taker. Experiment 1 showed that the chance of sealing the ear with earmolds made by the ordinary two-stage technique with only general buildup of the impression was approximately 10 percent and that none of the impression or earmold materials significantly improved the chance of obtaining a seal. Experiment 2 showed that the chance of obtaining a seal with multiple earmolds made from the one impression was zero, or very close to zero. Experiment 3 revealed that the chance of obtaining a seal increased to 55 percent when the earmold was made from an impression which was specially built up by the earmoldmaker. Experiment 4 showed that the chance of sealing the ear with earmolds made from multistage impressions was significantly greater (87.5 percent) than sealing the ear with those made from impressions specially built up by the earmoldmaker.

  5. Vacuum seals design and testing for a linear accelerator of the National Spallation Neutron Source

    SciTech Connect

    Z. Chen; C. Gautier; F. Hemez; N. K. Bultman

    2000-02-01

    Vacuum seals are very important to ensure that the Spallation Neutron Source (SNS) Linac has an optimum vacuum system. The vacuum joints between flanges must have reliable seals to minimize the leak rate and meet vacuum and electrical requirements. In addition, it is desirable to simplify the installation and thereby also simplify the maintenance required. This report summarizes an investigation of the metal vacuum seals that include the metal C-seal, Energized Spring seal, Helcoflex Copper Delta seal, Aluminum Delta seal, delta seal with limiting ring, and the prototype of the copper diamond seals. The report also contains the material certifications, design, finite element analysis, and testing for all of these seals. It is a valuable reference for any vacuum system design. To evaluate the suitability of several types of metal seals for use in the SNS Linac and to determine the torque applied on the bolts, a series of vacuum leak rate tests on the metal seals have been completed at Los Alamos Laboratory. A copper plated flange, using the same type of delta seal that was used for testing with the stainless steel flange, has also been studied and tested. A vacuum seal is desired that requires significantly less loading than a standard ConFlat flange with a copper gasket for the coupling cavity assembly. To save the intersegment space the authors use thinner flanges in the design. The leak rate of the thin ConFlat flange with a copper gasket is a baseline for the vacuum test on all seals and thin flanges. A finite element analysis of a long coupling cavity flange with a copper delta seal has been performed in order to confirm the design of the long coupling cavity flange and the welded area of a cavity body with the flange. This analysis is also necessary to predict a potential deformation of the cavity under the combined force of atmospheric pressure and the seating load of the seal. Modeling of this assembly has been achieved using both HKS/Abaqus and COSMOS

  6. Bearing-Cartridge Damping Seal

    NASA Technical Reports Server (NTRS)

    Goggins, David G.; Scharrer, Joseph K.; Chen, Wei C.

    1991-01-01

    In proposed design for improved ball-bearing cartridge, damping seal in form of thin-layer fluid journal bearing incorporated into cartridge. Damping seal acts as auxiliary bearing, relieving bearing balls of significant portions of both static and dynamic bearing loads. Damping from seal reduces dynamic loads even further by reducing amplitude of vibrations in second vibrational mode of rotor, which mode occurs when rotor turning at nearly full operating speed. Intended for use in high-pressure-oxygen turbopump of Space Shuttle main engine, also applicable to other turbomachinery bearings.

  7. Studying the Heat Shield's Seal

    NASA Technical Reports Server (NTRS)

    2005-01-01

    This image from NASA's Mars Exploration Rover Opportunity highlights the seal on the rover's protective heat shield. Engineers evaluated the performance of the protective shell's seal during a 36-sol investigation.

    After viewing these images, engineers were pleased with how the seal performed.

    This is an approximately true-color rendering of the scene acquired around 1:07 p.m. local solar time on Opportunity's sol 339 (Jan. 6, 2005) in an image mosaic using panoramic camera filters at wavelengths of 750, 530, and 430 nanometers.

  8. 7 CFR 29.35 - Lot seal.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Lot seal. 29.35 Section 29.35 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... INSPECTION Regulations Definitions § 29.35 Lot seal. A seal approved by the Director for sealing lots...

  9. 36 CFR 901.6 - Seal.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 3 2014-07-01 2014-07-01 false Seal. 901.6 Section 901.6... CORPORATION § 901.6 Seal. The Corporation may adopt a corporate seal which shall have the name of the Corporation and year of incorporation printed upon it. The seal may be used by causing it or a...

  10. 14 CFR 65.133 - Seal.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Seal. 65.133 Section 65.133 Aeronautics and...: AIRMEN OTHER THAN FLIGHT CREWMEMBERS Parachute Riggers § 65.133 Seal. Each certificated parachute rigger must have a seal with an identifying mark prescribed by the Administrator, and a seal press....

  11. 9 CFR 381.98 - Official seal.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 2 2013-01-01 2013-01-01 false Official seal. 381.98 Section 381.98... Certificates; Certification Procedures § 381.98 Official seal. The official mark for use in sealing means of... and a serial number as shown below, and any seals approved by the Administrator for applying such...

  12. 30 CFR 75.339 - Seals records.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Seals records. 75.339 Section 75.339 Mineral... SAFETY STANDARDS-UNDERGROUND COAL MINES Ventilation § 75.339 Seals records. (a) The table entitled “Seal.... Table—§ 75.339(a) Seal Recordkeeping Requirements Record Section reference Retention time (1)...

  13. 14 CFR 65.133 - Seal.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 2 2013-01-01 2013-01-01 false Seal. 65.133 Section 65.133 Aeronautics and...: AIRMEN OTHER THAN FLIGHT CREWMEMBERS Parachute Riggers § 65.133 Seal. Each certificated parachute rigger must have a seal with an identifying mark prescribed by the Administrator, and a seal press....

  14. 7 CFR 29.34 - Sample seal.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Sample seal. 29.34 Section 29.34 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... INSPECTION Regulations Definitions § 29.34 Sample seal. A seal approved by the Director for sealing...

  15. 36 CFR 901.6 - Seal.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 3 2012-07-01 2012-07-01 false Seal. 901.6 Section 901.6... CORPORATION § 901.6 Seal. The Corporation may adopt a corporate seal which shall have the name of the Corporation and year of incorporation printed upon it. The seal may be used by causing it or a...

  16. 14 CFR 65.133 - Seal.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Seal. 65.133 Section 65.133 Aeronautics and...: AIRMEN OTHER THAN FLIGHT CREWMEMBERS Parachute Riggers § 65.133 Seal. Each certificated parachute rigger must have a seal with an identifying mark prescribed by the Administrator, and a seal press....

  17. 46 CFR 501.11 - Official seal.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 9 2011-10-01 2011-10-01 false Official seal. 501.11 Section 501.11 Shipping FEDERAL... Seal § 501.11 Official seal. (a) Description. Pursuant to section 201(c) of the Merchant Marine Act, 1936, as amended (46 U.S.C. 301(d)), the Commission prescribes its official seal, as adopted by...

  18. 7 CFR 29.35 - Lot seal.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Lot seal. 29.35 Section 29.35 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... INSPECTION Regulations Definitions § 29.35 Lot seal. A seal approved by the Director for sealing lots...

  19. 9 CFR 381.98 - Official seal.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Official seal. 381.98 Section 381.98... Certificates; Certification Procedures § 381.98 Official seal. The official mark for use in sealing means of... and a serial number as shown below, and any seals approved by the Administrator for applying such...

  20. 19 CFR 101.7 - Customs seal.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 1 2014-04-01 2014-04-01 false Customs seal. 101.7 Section 101.7 Customs Duties U... GENERAL PROVISIONS § 101.7 Customs seal. (a) Design. According to the design furnished by the Department of the Treasury, the Customs seal of the United States shall consist of the seal of the Department...

  1. 7 CFR 29.34 - Sample seal.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Sample seal. 29.34 Section 29.34 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... INSPECTION Regulations Definitions § 29.34 Sample seal. A seal approved by the Director for sealing...

  2. 46 CFR 501.11 - Official seal.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 9 2014-10-01 2014-10-01 false Official seal. 501.11 Section 501.11 Shipping FEDERAL... Seal § 501.11 Official seal. (a) Description. Pursuant to section 201(c) of the Merchant Marine Act, 1936, as amended (46 U.S.C. 301(d)), the Commission prescribes its official seal, as adopted by...

  3. 30 CFR 75.339 - Seals records.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 1 2012-07-01 2012-07-01 false Seals records. 75.339 Section 75.339 Mineral... SAFETY STANDARDS-UNDERGROUND COAL MINES Ventilation § 75.339 Seals records. (a) The table entitled “Seal.... Table—§ 75.339(a) Seal Recordkeeping Requirements Record Section reference Retention time (1)...

  4. 30 CFR 75.339 - Seals records.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 1 2011-07-01 2011-07-01 false Seals records. 75.339 Section 75.339 Mineral... SAFETY STANDARDS-UNDERGROUND COAL MINES Ventilation § 75.339 Seals records. (a) The table entitled “Seal.... Table—§ 75.339(a) Seal Recordkeeping Requirements Record Section reference Retention time (1)...

  5. 46 CFR 501.11 - Official seal.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 9 2013-10-01 2013-10-01 false Official seal. 501.11 Section 501.11 Shipping FEDERAL... Seal § 501.11 Official seal. (a) Description. Pursuant to section 201(c) of the Merchant Marine Act, 1936, as amended (46 U.S.C. 301(d)), the Commission prescribes its official seal, as adopted by...

  6. 9 CFR 381.98 - Official seal.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 2 2012-01-01 2012-01-01 false Official seal. 381.98 Section 381.98... Certificates; Certification Procedures § 381.98 Official seal. The official mark for use in sealing means of... and a serial number as shown below, and any seals approved by the Administrator for applying such...

  7. 46 CFR 501.11 - Official seal.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 9 2012-10-01 2012-10-01 false Official seal. 501.11 Section 501.11 Shipping FEDERAL... Seal § 501.11 Official seal. (a) Description. Pursuant to section 201(c) of the Merchant Marine Act, 1936, as amended (46 U.S.C. 301(d)), the Commission prescribes its official seal, as adopted by...

  8. 14 CFR 65.133 - Seal.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Seal. 65.133 Section 65.133 Aeronautics and...: AIRMEN OTHER THAN FLIGHT CREWMEMBERS Parachute Riggers § 65.133 Seal. Each certificated parachute rigger must have a seal with an identifying mark prescribed by the Administrator, and a seal press....

  9. 19 CFR 101.7 - Customs seal.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 1 2011-04-01 2011-04-01 false Customs seal. 101.7 Section 101.7 Customs Duties U... GENERAL PROVISIONS § 101.7 Customs seal. (a) Design. According to the design furnished by the Department of the Treasury, the Customs seal of the United States shall consist of the seal of the Department...

  10. 7 CFR 29.35 - Lot seal.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Lot seal. 29.35 Section 29.35 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... INSPECTION Regulations Definitions § 29.35 Lot seal. A seal approved by the Director for sealing lots...

  11. 30 CFR 75.339 - Seals records.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 1 2013-07-01 2013-07-01 false Seals records. 75.339 Section 75.339 Mineral... SAFETY STANDARDS-UNDERGROUND COAL MINES Ventilation § 75.339 Seals records. (a) The table entitled “Seal.... Table—§ 75.339(a) Seal Recordkeeping Requirements Record Section reference Retention time (1)...

  12. 36 CFR 901.6 - Seal.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 3 2013-07-01 2012-07-01 true Seal. 901.6 Section 901.6... CORPORATION § 901.6 Seal. The Corporation may adopt a corporate seal which shall have the name of the Corporation and year of incorporation printed upon it. The seal may be used by causing it or a...

  13. 30 CFR 75.339 - Seals records.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 1 2014-07-01 2014-07-01 false Seals records. 75.339 Section 75.339 Mineral... SAFETY STANDARDS-UNDERGROUND COAL MINES Ventilation § 75.339 Seals records. (a) The table entitled “Seal.... Table—§ 75.339(a) Seal Recordkeeping Requirements Record Section reference Retention time (1)...

  14. 19 CFR 101.7 - Customs seal.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 1 2012-04-01 2012-04-01 false Customs seal. 101.7 Section 101.7 Customs Duties U... GENERAL PROVISIONS § 101.7 Customs seal. (a) Design. According to the design furnished by the Department of the Treasury, the Customs seal of the United States shall consist of the seal of the Department...

  15. 36 CFR 901.6 - Seal.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 3 2011-07-01 2011-07-01 false Seal. 901.6 Section 901.6... CORPORATION § 901.6 Seal. The Corporation may adopt a corporate seal which shall have the name of the Corporation and year of incorporation printed upon it. The seal may be used by causing it or a...

  16. 7 CFR 29.34 - Sample seal.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Sample seal. 29.34 Section 29.34 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... INSPECTION Regulations Definitions § 29.34 Sample seal. A seal approved by the Director for sealing...

  17. 9 CFR 381.98 - Official seal.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Official seal. 381.98 Section 381.98... Certificates; Certification Procedures § 381.98 Official seal. The official mark for use in sealing means of... and a serial number as shown below, and any seals approved by the Administrator for applying such...

  18. 7 CFR 29.34 - Sample seal.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Sample seal. 29.34 Section 29.34 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... INSPECTION Regulations Definitions § 29.34 Sample seal. A seal approved by the Director for sealing...

  19. 19 CFR 101.7 - Customs seal.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 1 2013-04-01 2013-04-01 false Customs seal. 101.7 Section 101.7 Customs Duties U... GENERAL PROVISIONS § 101.7 Customs seal. (a) Design. According to the design furnished by the Department of the Treasury, the Customs seal of the United States shall consist of the seal of the Department...

  20. 46 CFR 501.11 - Official seal.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 9 2010-10-01 2010-10-01 false Official seal. 501.11 Section 501.11 Shipping FEDERAL... Seal § 501.11 Official seal. (a) Description. Pursuant to section 201(c) of the Merchant Marine Act, 1936, as amended (46 U.S.C. 301(d)), the Commission prescribes its official seal, as adopted by...

  1. 9 CFR 381.98 - Official seal.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 2 2014-01-01 2014-01-01 false Official seal. 381.98 Section 381.98... Certificates; Certification Procedures § 381.98 Official seal. The official mark for use in sealing means of... and a serial number as shown below, and any seals approved by the Administrator for applying such...

  2. 14 CFR 65.133 - Seal.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 2 2014-01-01 2014-01-01 false Seal. 65.133 Section 65.133 Aeronautics and...: AIRMEN OTHER THAN FLIGHT CREWMEMBERS Parachute Riggers § 65.133 Seal. Each certificated parachute rigger must have a seal with an identifying mark prescribed by the Administrator, and a seal press....

  3. 7 CFR 29.35 - Lot seal.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Lot seal. 29.35 Section 29.35 Agriculture Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing... INSPECTION Regulations Definitions § 29.35 Lot seal. A seal approved by the Director for sealing lots...

  4. 36 CFR 901.6 - Seal.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Seal. 901.6 Section 901.6... CORPORATION § 901.6 Seal. The Corporation may adopt a corporate seal which shall have the name of the Corporation and year of incorporation printed upon it. The seal may be used by causing it or a...

  5. Overview of Turbine Seal Testing at GRC

    NASA Technical Reports Server (NTRS)

    Proctor, Margaret P.; Delgado, Irebert R.

    2006-01-01

    Objectives: 1) Evaluate feasibility of advanced seal concepts and materials of meeting next generation engine speed and temperature requirements; 2) Provide a state-of-the-art turbomachinery seal test rig capable of testing seals under known and anticipated design conditions; and 3) Work with industry to assess and demonstrate performance of their seals prior to test in engine.

  6. Seal device for ferromagnetic containers

    DOEpatents

    Meyer, Ross E.; Jason, Andrew J.

    1994-01-01

    A temporary seal or patch assembly prevents the escape of contents, e.g., fluids and the like, from within a container having a breach therethrough until the contents can be removed and/or a repair effected. A frame that supports a sealing bladder can be positioned over the breach and the frame is then attached to the container surface, which must be of a ferromagnet material, by using switchable permanent magnets. The permanent magnets are designed to have a first condition that is not attracted to the ferromagnetic surface and a second conditions whereby the magnets are attracted to the surface with sufficient force to support the seal assembly on the surface. Latching devices may be attached to the frame and engage the container surface with hardened pins to prevent the lateral movement of the seal assembly along the container surface from external forces such as fluid drag or gravity.

  7. Seal device for ferromagnetic containers

    DOEpatents

    Meyer, R.E.; Jason, A.J.

    1994-10-18

    A temporary seal or patch assembly prevents the escape of contents, e.g., fluids and the like, from within a container having a breach there through until the contents can be removed and/or a repair effected. A frame that supports a sealing bladder can be positioned over the breach and the frame is then attached to the container surface, which must be of a ferromagnet material, by using switchable permanent magnets. The permanent magnets are designed to have a first condition that is not attracted to the ferromagnetic surface and a second conditions whereby the magnets are attracted to the surface with sufficient force to support the seal assembly on the surface. Latching devices may be attached to the frame and engage the container surface with hardened pins to prevent the lateral movement of the seal assembly along the container surface from external forces such as fluid drag or gravity. 10 figs.

  8. Cryogenic Brush Seal Test Results

    NASA Technical Reports Server (NTRS)

    Proctor, Margaret P.; Walker, James F.

    1996-01-01

    Brush seals are compliant, contact seals that have long-life, low-leakage characteristics desirable for use in rocket engine turbopumps. 50.8-mm (2.0 inch) diameter brush seals with a nominal initial radial interference of 0.127-mm (0.005 inch) were tested in liquid nitrogen at shaft speeds up to 35,000 rpm and differential pressure loads up to 1.21 MPa (175 psi) per brush. The measured leakage rate of a single brush was 2-3 times less than that measured for a 12-tooth, 0.127-mm (0.005 inch) radial clearance labyrinth seal used as a baseline. Stage effects were studied and it was found that two brush seals with a large separation distance leaked less than two brushes tightly packed together. The maximum measured groove depth on the Inconel 718 rotor was 25.4 (mu)m (0.001 inch) after 4.31 hours of shaft rotation. The Haynes-25 bristles wore approximately 25.4-76.2 (mu)m (0.001-0.003 inch) under the same conditions. Three seal runner coatings, chromium carbide, Teflon impregnated chromium, and zirconium oxide, were tested in liquid hydrogen at 35,000 and 65,000 rpm with separate 50.8 mm diameter brush seals made of Haynes-25 bristles and having a nominal initial radial interference of 129 rpm. Two bare Inconel-718 rotors were also tested as a baseline. The test results revealed significant differences between the wear characteristics of the uncoated and coated seal runners. At both speeds the brush seal with the bare Inconel-718 seal runner exhibited significant bristle wear with excessive material transferring to the runner surface. In contrast, the coated seal runners inhibited the transfer and deposit of bristle material. The chromium carbide coating showed only small quantities of bristle material transferring to its surface. The Teflon impregnated chromium coating also inhibited material transfer and provided some lubrication. This coating, however, is self-sacrificing. The Teflon remained present on the low speed runner, but it was completely removed from the

  9. Remotely Monitored Sealing Array Software

    SciTech Connect

    2012-09-12

    The Remotely Monitored Sealing Array (RMSA) utilizes the Secure Sensor Platform (SSP) framework to establish the fundamental operating capabilities for communication, security, power management, and cryptography. In addition to the SSP framework the RMSA software has unique capabilities to support monitoring a fiber optic seal. Fiber monitoring includes open and closed as well as parametric monitoring to detect tampering attacks. The fiber monitoring techniques, using the SSP power management processes, allow the seals to last for years while maintaining the security requirements of the monitoring application. The seal is enclosed in a tamper resistant housing with software to support active tamper monitoring. New features include LED notification of fiber closure, the ability to retrieve the entire fiber optic history via translator command, separate memory storage for fiber optic events, and a more robust method for tracking and resending failed messages.

  10. Reactor vessel seal service fixture

    DOEpatents

    Ritz, W.C.

    1975-12-01

    An apparatus for the preparation of exposed sealing surfaces along the open rim of a nuclear reactor vessel comprised of a motorized mechanism for traveling along the rim and simultaneously brushing the exposed surfaces is described.

  11. Seals Research at AlliedSignal

    NASA Technical Reports Server (NTRS)

    Ullah, M. Rifat

    1996-01-01

    A consortium has been formed to address seal problems in the Aerospace sector of Allied Signal, Inc. The consortium is represented by makers of Propulsion Engines, Auxiliary Power Units, Gas Turbine Starters, etc. The goal is to improve Face Seal reliability, since Face Seals have become reliability drivers in many of our product lines. Several research programs are being implemented simultaneously this year. They include: Face Seal Modeling and Analysis Methodology; Oil Cooling of Seals; Seal Tracking Dynamics; Coking Formation & Prevention; and Seal Reliability Methods.

  12. New-Generation Sealing Slurries For Borehole Injection Purposes

    NASA Astrophysics Data System (ADS)

    Stryczek, Stanisław; Gonet, Andrzej; Wiśniowski, Rafał; Złotkowski, Albert

    2015-12-01

    The development of techniques and technologies thanks to which parameters of the ground medium can be modified makes specialists look for new recipes of geopolymers - binders for the reinforcing and sealing of unstable and permeable grounds. The sealing slurries are expected to meet a number of strict requirements, therefore it is important to find new admixtures and additives which could modify the fresh and hardened slurry. Special attention has been recently paid to the fluid ash - a by-product of the combustion of hard coals. However, the use of this additive is associated with the application of appropriate superplastifier. Laboratory analyses of rheological parameters of fresh sealing slurries and the ways of improving their liquidity by a properly selected third-generation superplastifier are presented in the paper. The slurries were based on Portland cement CEM I, milled granulated large-furnace slag and fly ash from fluidized-bed combustion of hard coal.

  13. Seal Monitoring System for an Explosive Containment Vessel

    SciTech Connect

    Pastrnak, J W; Henning, C D; Switzer, V A; Grundler, W; Holloway, J R; Morrison, J J; Hafner, R S

    2004-06-28

    Researchers at Lawrence Livermore National Laboratory are developing high-performance explosive firing vessels to contain (one time) explosive detonations that contain toxic metals and hazardous gases. The filament-wound polymer composite vessels are designed to contain up to 80 lb (TNT equivalent) explosive in a 2-meter sphere without leakage. So far, two half-scale (1-meter diameter) vessels have been tested; one up to 150% of the design explosive limit. Peak dynamic pressures in excess of 280 MPa (40 Ksi) in the vessel were calculated and measured. Results indicated that there was a small amount of gas and particle leakage past the first two of the seven o-ring seals. However, the remaining five seals prevented any transient leakage of the toxic gases and particulates out of the vessel. These results were later confirmed by visual inspection and particulate analysis of swipes taken from the sealing surfaces.

  14. Brush Seals for Improved Steam Turbine Performance

    NASA Technical Reports Server (NTRS)

    Turnquist, Norman; Chupp, Ray; Baily, Fred; Burnett, Mark; Rivas, Flor; Bowsher, Aaron; Crudgington, Peter

    2006-01-01

    GE Energy has retrofitted brush seals into more than 19 operating steam turbines. Brush seals offer superior leakage control compared to labyrinth seals, owing to their compliant nature and ability to maintain very tight clearances to the rotating shaft. Seal designs have been established for steam turbines ranging in size from 12 MW to over 1200 MW, including fossil, nuclear, combined-cycle and industrial applications. Steam turbines present unique design challenges that must be addressed to ensure that the potential performance benefits of brush seals are realized. Brush seals can have important effects on the overall turbine system that must be taken into account to assure reliable operation. Subscale rig tests are instrumental to understanding seal behavior under simulated steam-turbine operating conditions, prior to installing brush seals in the field. This presentation discusses the technical challenges of designing brush seals for steam turbines; subscale testing; performance benefits of brush seals; overall system effects; and field applications.

  15. The 2000 canine distemper epidemic in Caspian seals (Phoca caspica): pathology and analysis of contributory factors.

    PubMed

    Kuiken, T; Kennedy, S; Barrett, T; Van de Bildt, M W G; Borgsteede, F H; Brew, S D; Codd, G A; Duck, C; Deaville, R; Eybatov, T; Forsyth, M A; Foster, G; Jepson, P D; Kydyrmanov, A; Mitrofanov, I; Ward, C J; Wilson, S; Osterhaus, A D M E

    2006-05-01

    More than 10,000 Caspian seals (Phoca caspica) were reported dead in the Caspian Sea during spring and summer 2000. We performed necropsies and extensive laboratory analyses on 18 seals, as well as examination of the pattern of strandings and variation in weather in recent years, to identify the cause of mortality and potential contributory factors. The monthly stranding rate in 2000 was up to 2.8 times the historic mean. It was preceded by an unusually mild winter, as observed before in mass mortality events of pinnipeds. The primary diagnosis in 11 of 13 seals was canine distemper, characterized by broncho-interstitial pneumonia, lymphocytic necrosis and depletion in lymphoid organs, and the presence of typical intracytoplasmic inclusion bodies in multiple epithelia. Canine distemper virus infection was confirmed by phylogenetic analysis of reverse transcriptase-polymerase chain reaction products. Organochlorine and zinc concentrations in tissues of seals with canine distemper were comparable to those of Caspian seals in previous years. Concurrent bacterial infections that may have contributed to the mortality of the seals included Bordetella bronchiseptica (4/8 seals), Streptococcus phocae (3/8), Salmonella dublin (1/8), and S. choleraesuis (1/8). A newly identified bacterium, Corynebacterium caspium, was associated with balanoposthitis in one seal. Several infectious and parasitic organisms, including poxvirus, Atopobacter phocae, Eimeria- and Sarcocystis-like organisms, and Halarachne sp. were identified in Caspian seals for the first time.

  16. TOF-SIMS study of red sealing-inks on paper and its forensic applications

    NASA Astrophysics Data System (ADS)

    Lee, Jihye; Lee, Chiwoo; Lee, Kangbong; Lee, Yeonhee

    2008-12-01

    Red sealing-ink samples are frequently submitted to forensic laboratories for identification and comparison in a wide variety of criminal and civil cases. The non-destructive identification of sealing-inks on paper is of important to preserve as large an amount of a fraudulent check, forged document, or other such evidence as possible for use in legal proceedings. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) for the analysis of red sealing-inks on paper surfaces was investigated. TOF-SIMS spectra were employed to identify elements and organic compounds in red sealing-inks. An analysis was performed on five sealing-inks of red color manufactured in Korea, Japan, and China. Several fibers from specific sealing-ink sample on paper can be removed leaving little evidence of tampering and can be distinguished from other sealing-inks. Overlapped area of sealing-ink and ballpoint pen in the document was also investigated in order to identify the sequence of recording. Therefore, this study shows that TOF-SIMS is a useful technique for the non-destructive analysis of red sealing-inks on paper.

  17. Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b.

    PubMed Central

    Tsien, H C; Brusseau, G A; Hanson, R S; Waclett, L P

    1989-01-01

    The methanotroph Methylosinus trichosporium OB3b, a type II methanotroph, degraded trichloroethylene at rates exceeding 1.2 mmol/h per g (dry weight) following the appearance of soluble methane monooxygenase in continuous and batch cultures. Cells capable oxidizing trichloroethylene contained components of soluble methane monooxygenase as demonstrated by Western blot (immunoblot) analysis with antibodies prepared against the purified enzyme. Growth of cultures in a medium containing 0.25 microM or less copper sulfate caused derepression of the synthesis of soluble methane monooxygenase. In these cultures, the specific rates of methane and methanol oxidation did not change during growth, while trichloroethylene oxidation increased with the appearance of soluble methane monooxygenase. M. trichosporium OB3b cells that contained soluble methane monooxygenase also degraded vinyl chloride, 1,1-dichloroethylene, cis-1,2-dichloroethylene, and trans-1,2-dichloroethylene. Images PMID:2515801

  18. Reusable, tamper-indicating seal

    DOEpatents

    Ryan, Michael J.

    1978-01-01

    A reusable, tamper-indicating seal comprises a drum confined within a fixed body and rotatable in one direction therewithin, the top of the drum constituting a tray carrying a large number of small balls of several different colors. The fixed body contains parallel holes for looping a seal wire therethrough. The base of the drums carries cams adapted to coact with cam followers to lock the wire within the seal at one angular position of the drum. A channel in the fixed body -- visible from outside the seal -- adjacent the tray constitutes a segregated location for a small plurality of the colored balls. A spring in the tray forces colored balls into the segregated location at one angular position of the drum, further rotation securing the balls in position and the wires in the seal. A wedge-shaped plough removes the balls from the segregated location, at a different angular position of the drum, the wire being unlocked at the same position. A new pattern of colored balls will appear in the segregated location when the seal is relocked.

  19. Probe Insertion Apparatus with Inflatable Seal

    NASA Technical Reports Server (NTRS)

    Trimarchi, Paul A.

    1991-01-01

    A sealing apparatus for inserting a probe into a pressure vessel having an elongated opening includes Ii pair of resiliently defQrmable seals opposingly disposed in sealing engagement with each other. A retainer is connected to the pressure vessel around the elongated opening and holds the pair of seals rigidly to the pressure vessel. A wedge is engageable with the pair of seals and carries the probe, for longitudinally translating the probe in the pressure vessel.

  20. APOSTLE: 11 TRANSIT OBSERVATIONS OF TrES-3b

    SciTech Connect

    Kundurthy, P.; Becker, A. C.; Agol, E.; Barnes, R.; Williams, B.

    2013-02-10

    The Apache Point Survey of Transit Lightcurves of Exoplanets (APOSTLE) observed 11 transits of TrES-3b over two years in order to constrain system parameters and look for transit timing and depth variations. We describe an updated analysis protocol for APOSTLE data, including the reduction pipeline, transit model, and Markov Chain Monte Carlo analyzer. Our estimates of the system parameters for TrES-3b are consistent with previous estimates to within the 2{sigma} confidence level. We improved the errors (by 10%-30%) on system parameters such as the orbital inclination (i {sub orb}), impact parameter (b), and stellar density ({rho}{sub *}) compared to previous measurements. The near-grazing nature of the system, and incomplete sampling of some transits, limited our ability to place reliable uncertainties on individual transit depths and hence we do not report strong evidence for variability. Our analysis of the transit timing data shows no evidence for transit timing variations and our timing measurements are able to rule out super-Earth and gas giant companions in low-order mean motion resonance with TrES-3b.

  1. Hydrodynamic perception in true seals (Phocidae) and eared seals (Otariidae).

    PubMed

    Hanke, Wolf; Wieskotten, Sven; Marshall, Christopher; Dehnhardt, Guido

    2013-06-01

    Pinnipeds, that is true seals (Phocidae), eared seals (Otariidae), and walruses (Odobenidae), possess highly developed vibrissal systems for mechanoreception. They can use their vibrissae to detect and discriminate objects by direct touch. At least in Phocidae and Otariidae, the vibrissae can also be used to detect and analyse water movements. Here, we review what is known about this ability, known as hydrodynamic perception, in pinnipeds. Hydrodynamic perception in pinnipeds developed convergently to the hydrodynamic perception with the lateral line system in fish and the sensory hairs in crustaceans. So far two species of pinnipeds, the harbour seal (Phoca vitulina) representing the Phocidae and the California sea lion (Zalophus californianus) representing the Otariidae, have been studied for their ability to detect local water movements (dipole stimuli) and to follow hydrodynamic trails, that is the water movements left behind by objects that have passed by at an earlier point in time. Both species are highly sensitive to dipole stimuli and can follow hydrodynamic trails accurately. In the individuals tested, California sea lions were clearly more sensitive to dipole stimuli than harbour seals, and harbour seals showed a superior trail following ability as compared to California sea lions. Harbour seals have also been shown to derive additional information from hydrodynamic trails, such as motion direction, size and shape of the object that caused the trail (California sea lions have not yet been tested). The peculiar undulated shape of the harbour seals' vibrissae appears to play a crucial role in trail following, as it suppresses self-generated noise while the animal is swimming.

  2. Overview of NASA Glenn Seal Program

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M.; Proctor, Margaret P.; Dunlap, Patrick H., Jr.; Delgado, Irebert; DeMange, Jeffrey J.; Daniels, Christopher C.; Lattime, Scott B.

    2003-01-01

    The Seal Team is divided into four primary areas. These areas include turbine engine seal development, structural seal development, acoustic seal development, and adaptive seal development. The turbine seal area focuses on high temperature, high speed shaft seals for secondary air system flow management. The structural seal area focuses on high temperature, resilient structural seals required to accommodate large structural distortions for both space- and aero-applications. Our goal in the acoustic seal project is to develop non-contacting, low leakage seals exploiting the principles of advanced acoustics. We are currently investigating a new acoustic field known as Resonant Macrosonic Synthesis (RMS) to see if we can harness the large acoustic standing pressure waves to form an effective air-barrier/seal. Our goal in the adaptive seal project is to develop advanced sealing approaches for minimizing blade-tip (shroud) or interstage seal leakage. We are planning on applying either rub-avoidance or regeneration clearance control concepts (including smart structures and materials) to promote higher turbine engine efficiency and longer service lives.

  3. RADIOLOGICAL SEALED SOURCE LIBRARY: A NUCLEAR FORENSICS TOOL

    SciTech Connect

    Canaday, Jodi; Chamberlain, David; Finck, Martha; Carney, Kevin

    2015-01-01

    If a terrorist were to obtain and possibly detonate a device that contained radiological material, radiological forensic analysis of the material and source capsule could provide law enforcement with valuable clues about the origin of the radiological material; this information could then provide further leads on where the material and sealed source was obtained, and the loss of control point. This information could potentially be utilized for attribution and prosecution. Analyses of nuclear forensic signatures for radiological materials are generally understood to include isotopic ratios, trace element concentrations, the time since irradiation or purification, and morphology. Radiological forensic signatures for sealed sources provide additional information that leverages information on the physical design and chemical composition of the source capsule and containers, physical markings indicative of an owner or manufacturer. Argonne National Laboratory (Argonne), in collaboration with Idaho National Laboratory (INL), has been working since 2003 to understand signatures that could be used to identify specific source manufacturers. These signatures include the materials from which the capsule is constructed, dimensions, weld details, elemental composition, and isotopic abundances of the radioactive material. These signatures have been compiled in a library known as the Argonne/INL Radiological Sealed Source Library. Data collected for the library has included open-source information from vendor catalogs and web pages; discussions with source manufacturers and touring of production facilities (both protected through non-disclosure agreements); technical publications; and government registries such as the U.S. Nuclear Regulatory Commission’s Sealed Source and Device Registry.

  4. Using Sealed Wells to Measure Water Levels Beneath Streams and Floodplains.

    PubMed

    Noorduijn, Saskia L; Cook, Peter G; Wood, Cameron; White, Nick

    2015-01-01

    The design of wells beneath streams and floodplains has often employed with tall standpipes to prevent incursion of surface water into the well during flood events. Here, an approach has been presented to minimise the infrastructure demands in these environments by sealing the well top (e.g., prevent water entering the well) and monitor the total pressure in the water column using an absolute (non-vented) pressure transducer. The sealed well design was tested using a laboratory experiment where the total pressure responses were monitored in both an unsealed and sealed well, while the water level was varied. It is observed that, whether the well is sealed or not, the total pressure at a given depth in the aquifer will be equal to that within the well. This indicates that the sealed well design is a viable alternative to tall standpipes and also facilitates installation of wells beneath streams and floodplains.

  5. An experimental technique for performing 3-D LDA measurements inside whirling annular seals

    NASA Technical Reports Server (NTRS)

    Morrison, Gerald L.; Johnson, Mark C.; Deotte, Robert E., Jr.; Thames, H. Davis, III.; Wiedner, Brian G.

    1992-01-01

    During the last several years, the Fluid Mechanics Division of the Turbomachinery Laboratory at Texas A&M University has developed a rather unique facility with the experimental capability for measuring the flow field inside journal bearings, labyrinth seals, and annular seals. The facility consists of a specially designed 3-D LDA system which is capable of measuring the instantaneous velocity vector within 0.2 mm of a wall while the laser beams are aligned almost perpendicular to the wall. This capability was required to measure the flow field inside journal bearings, labyrinth seals, and annular seals. A detailed description of this facility along with some representative results obtained for a whirling annular seal are presented.

  6. Canister, sealing method and composition for sealing a borehole

    SciTech Connect

    Brown, Donald W.; Wagh, Arun S.

    2003-05-13

    Canister, sealing method and composition for sealing a borehole. The canister includes a container with slurry inside the container, one or more slurry exits at one end of the container, a pump at the other end of the container, and a piston inside that pushes the slurry though the slurry exit(s), out of the container, and into a borehole. An inflatable packer outside the container provides stabilization in the borehole. A borehole sealing material is made by combining an oxide or hydroxide and a phosphate with water to form a slurry which then sets to form a high strength, minimally porous material which binds well to itself, underground formations, steel and ceramics.

  7. Standards Laboratory environments

    SciTech Connect

    Braudaway, D.W.

    1990-09-01

    Standards Laboratory environments need to be carefully selected to meet the specific mission of each laboratory. The mission of the laboratory depends on the specific work supported, the measurement disciplines required and the level of uncertainty required in the measurements. This document reproduces the contents of the Sandia National Laboratories Primary Standards Laboratory Memorandum Number 3B (PSLM-3B) which was issued on May 16, 1988, under the auspices of the Department of Energy, Albuquerque Operations Office, to guide the laboratories of the Nuclear Weapons Complex in selecting suitable environments. Because of both general interest and specific interest in Standards Laboratory environments this document is being issued in a more available form. The purpose of this document is to provide guidance in selection of laboratory environments suitable for standards maintenance and calibration operations. It is not intended to mandate a specific environment for a specific calibration but to direct selection of the environment and to offer suggestions on how to extend precision in an existing and/or achievable (practical) environment. Although this documents pertains specifically to standards laboratories, it can be applied to any laboratory requiring environmental control.

  8. Tamper-Indicating Quantum Seal*

    NASA Astrophysics Data System (ADS)

    Williams, Brian P.; Britt, Keith A.; Humble, Travis S.

    2016-01-01

    Technical means for identifying when tampering occurs is a critical part of many containment and surveillance technologies. Conventional fiber-optic seals provide methods for monitoring enclosed inventories, but they are vulnerable to spoofing attacks based on classical physics. We address these vulnerabilities with the development of a quantum seal that offers the ability to detect the intercept-resend attack using quantum integrity verification. Our approach represents an application of entanglement to provide guarantees in the authenticity of the seal state by verifying it is transmitted coherently. We implement these ideas using polarization-entangled photon pairs that are verified after passing through a fiber-optic-channel test bed. Using binary-detection theory, we find the probability of detecting inauthentic signals is greater than 0.9999 with a false-alarm chance of 1 0-9 for a 10-s sampling interval. In addition, we show how the Hong-Ou-Mandel effect concurrently provides a tight bound on redirection attack, in which tampering modifies the shape of the seal. Our measurements limit the tolerable path-length change to submillimeter disturbances. These tamper-indicating features of the quantum seal offer unprecedented security for unattended monitoring systems.

  9. Crystal structure of inactive form of Rab3B

    SciTech Connect

    Zhang, Wei; Shen, Yang; Jiao, Ronghong; Liu, Yanli; Deng, Lingfu; Qi, Chao

    2012-06-28

    Rab proteins are the largest family of ras-related GTPases in eukaryotic cells. They act as directional molecular switches at membrane trafficking, including vesicle budding, cargo sorting, transport, tethering, and fusion. Here, we generated and crystallized the Rab3B:GDP complex. The structure of the complex was solved to 1.9 {angstrom} resolution and the structural base comparison with other Rab3 members provides a structural basis for the GDP/GTP switch in controlling the activity of small GTPase. The comparison of charge distribution among the members of Rab3 also indicates their different roles in vesicular trafficking.

  10. Nonelastomeric Rod Seals for Advanced Hydraulic Systems

    NASA Technical Reports Server (NTRS)

    Hady, W. F.; Waterman, A. W.

    1976-01-01

    Advanced high temperature hydraulic system rod sealing requirements can be met by using seals made of nonelastomeric (plastic) materials in applications where elastomers do not have adequate life. Exploratory seal designs were optimized for advanced applications using machinable polyimide materials. These seals demonstrated equivalent flight hour lives of 12,500 at 350 F and 9,875 at 400 F in advanced hydraulic system simulation. Successful operation was also attained under simulated space shuttle applications; 96 reentry thermal cycles and 1,438 hours of vacuum storage. Tests of less expensive molded plastic seals indicated a need for improved materials to provide equivalent performance to the machined seals.

  11. Gas Path Sealing in Turbine Engines

    NASA Technical Reports Server (NTRS)

    Ludwig, L. P.

    1978-01-01

    A survey of gas path seals is presented with particular attention given to sealing clearance effects on engine component efficiency. The effects on compressor pressure ratio and stall margin are pointed out. Various case-rotor relative displacements, which affect gas path seal clearances, are identified. Forces produced by nonuniform sealing clearances and their effect on rotor stability are discussed qualitatively, and recent work on turbine-blade-tip sealing for high temperature is described. The need for active clearance control and for engine structural analysis is discussed. The functions of the internal-flow system and its seals are reviewed.

  12. Monolithic LTCC seal frame and lid

    SciTech Connect

    Krueger, Daniel S.; Peterson, Kenneth A.; Stockdale, Dave; Duncan, James Brent; Riggs, Bristen

    2016-06-21

    A method for forming a monolithic seal frame and lid for use with a substrate and electronic circuitry comprises the steps of forming a mandrel from a ceramic and glass based material, forming a seal frame and lid block from a ceramic and glass based material, creating a seal frame and lid by forming a compartment and a plurality of sidewalls in the seal frame and lid block, placing the seal frame and lid on the mandrel such that the mandrel fits within the compartment, and cofiring the seal frame and lid block.

  13. Actively controlled shaft seals for aerospace applications

    NASA Technical Reports Server (NTRS)

    Salant, Richard F.

    1991-01-01

    Actively controlled mechanical seals have recently been developed for industrial use. This study investigates the feasibility of using such seals for aerospace applications. In a noncontacting mechanical seal, the film thickness depends on the geometry of the seal interface. The amount of coning, which is a measure of the radial convergence or divergence of the seal interface, has a primary effect on the film thickness. Active control of the film thickness is established by controlling the coning with a piezoelectric material. A mathematical model has been formulated to predict the performance of an actively controlled mechanical seal.

  14. Labyrinth seal testing for lift fan engines

    NASA Technical Reports Server (NTRS)

    Dobek, L. J.

    1973-01-01

    An abradable buffered labyrinth seal for the control of turbine gas path leakage in a tip-turbine driven lift fan was designed, tested, and analyzed. The seal configuration was not designed to operate in any specific location but was sized to be evaluated in an existing test rig. The final sealing diameter selected was 28 inches. Results of testing indicate that the flow equations predicted seal air flows consistent with measured values. Excellent sealing characteristics of the abradable coating on the stator land were demonstrated when a substantial seal penetration of .030 inch into the land surface was encountered without appreciable wear on the labyrinth knife edges.

  15. Cryogenic Flange and Seal Evaluation

    NASA Technical Reports Server (NTRS)

    Ramirez, Adrian

    2014-01-01

    The assembly of flanges, seals, and pipes are used to carry cryogenic fluid from a storage tank to the vehicle at launch sites. However, after a certain amount of cycles these raised face flanges with glass-filled Teflon gaskets have been found to have torque relaxation and are as a result susceptible to cryogenic fluid leakage if not re-torqued. The intent of this project is to identify alternate combinations of flanges and seals which may improve thermal cycle performance and decrease re-torque requirements. The general approach is to design a test fixture to evaluate leak characteristics between spiral and concentric serrations and to test alternate flange and seal combinations. Due to insufficient time, it was not possible to evaluate these different types of combinations for the combination that improved thermal cycle performance the most. However, the necessary drawings for the test fixture were designed and assembled along with the collection of the necessary parts.

  16. Laser sealed vacuum insulation window

    DOEpatents

    Benson, David K.; Tracy, C. Edwin

    1987-01-01

    A laser sealed evacuated window panel is comprised of two glass panes held spaced apart in relation to each other by a plurality of spherical glass beads and glass welded around the edges to provide an evacuated space between the glass panes that is completely glass sealed from the exterior. The glass welded edge seal is obtained by welding the edges of the glass panes together with a laser beam while the glass panes and bead spacers are positioned in a vacuum furnace and heated to the annealing point of the glass to avoid stress fracture in the area of the glass weld. The laser welding in the furnace can be directed around the perimeter of the glass panel by a combination of rotating the glass panel and linearly translating or aiming the laser with a relay mirror.

  17. Mechanical seal with textured sidewall

    DOEpatents

    Khonsari, Michael M.; Xiao, Nian

    2017-02-14

    The present invention discloses a mating ring, a primary ring, and associated mechanical seal having superior heat transfer and wear characteristics. According to an exemplary embodiment of the present invention, one or more dimples are formed onto the cylindrical outer surface of a mating ring sidewall and/or a primary ring sidewall. A stationary mating ring for a mechanical seal assembly is disclosed. Such a mating ring comprises an annular body having a central axis and a sealing face, wherein a plurality of dimples are formed into the outer circumferential surface of the annular body such that the exposed circumferential surface area of the annular body is increased. The texture added to the sidewall of the mating ring yields superior heat transfer and wear characteristics.

  18. Energy conservation through sealing technology

    NASA Technical Reports Server (NTRS)

    Stair, W. K.; Ludwig, L. P.

    1978-01-01

    Improvements in fluid film sealing resulting from a proposed research program could lead to an annual energy saving, on a national basis, equivalent to about 37 million bbl of oil or 0.3% of the total U.S. energy consumption. Further, the application of known sealing technology can result in an annual saving of an additional 10 million bbl of oil. The energy saving would be accomplished by reduction in process heat energy loss, reduction of frictional energy generated, and minimization of energy required to operate ancillary equipment associated with the seal system. In addition to energy saving, cost effectiveness is further enhanced by reduction in maintenance and in minimization of equipment for collecting leakage and for meeting environmental pollution standards.

  19. Laser sealed vacuum insulating window

    DOEpatents

    Benson, D.K.; Tracy, C.E.

    1985-08-19

    A laser sealed evacuated window panel is comprised of two glass panes held spaced apart in relation to each other by a plurality of spherical glass beads and glass welded around the edges to provide an evacuated space between the glass panes that is completely glass sealed from the exterior. The glass welded edge seal is obtained by welding the edges of the glass panes together with a laser beam while the glass panes and bead spacers are positioned in a vacuum furnace and heated to the annealing point of the glass to avoid stress fracture in the area of the glass weld. The laser welding in the furnace can be directed around the perimeter of the galss panel by a combination of rotating the glass panel and linearly translating or aiming the laser with a relay mirror.

  20. Analysis of methane biodegradation by Methylosinus trichosporium OB3b

    PubMed Central

    Rodrigues, Andréa dos Santos; Salgado, Belkis Valdman e Andréa Medeiros

    2009-01-01

    The microbial oxidation of methane in the atmosphere is performed by methanotrophic bacteria that use methane as a unique source of carbon and energy. The objective of this work consisted of the investigation of the best conditions of methane biodegradation by methanotrophic bacteria Methylosinus trichosporium OB3b that oxidize it to carbon dioxide, and the use of these microorganisms in monitoring methods for methane. The results showed that M. trichosporium OB3b was capable to degrade methane in a more effective way with an initial microorganism concentration of 0.0700 g.L-1, temperature of 30ºC, pH 6.5 and using 1.79 mmol of methane. In these same conditions, there was no bacterial growth when 2.69 mmol of methane was used. The specific rate of microorganism growth, the conversion factor, the efficiency and the volumetric productivity, for the optimized conditions of biodegradation were, respectively, 0.0324 h-1, 0.6830 gcells/gCH4, 73.73% and 2.7732.10-3 gcells/L.h. The final product of methane microbiological degradation, carbon dioxide, was quantified through the use of a commercial electrode, and, through this, the grade of methane conversion in carbon dioxide was calculated. PMID:24031362

  1. Stabilizing geometry for hydrodynamic rotary seals

    DOEpatents

    Dietle, Lannie L.; Schroeder, John E.

    2010-08-10

    A hydrodynamic sealing assembly including a first component having first and second walls and a peripheral wall defining a seal groove, a second component having a rotatable surface relative to said first component, and a hydrodynamic seal comprising a seal body of generally ring-shaped configuration having a circumference. The seal body includes hydrodynamic and static sealing lips each having a cross-sectional area that substantially vary in time with each other about the circumference. In an uninstalled condition, the seal body has a length defined between first and second seal body ends which varies in time with the hydrodynamic sealing lip cross-sectional area. The first and second ends generally face the first and second walls, respectively. In the uninstalled condition, the first end is angulated relative to the first wall and the second end is angulated relative to the second wall. The seal body has a twist-limiting surface adjacent the static sealing lip. In the uninstalled condition, the twist-limiting surface is angulated relative to the peripheral wall and varies along the circumference. A seal body discontinuity and a first component discontinuity mate to prevent rotation of the seal body relative to the first component.

  2. High Pressure Rotary Shaft Sealing Mechanism

    DOEpatents

    Dietle, Lannie; Gobeli, Jeffrey D.

    2001-05-08

    A laterally translatable pressure staged rotary shaft sealing mechanism having a seal housing with a shaft passage therein being exposed to a fluid pressure P1 and with a rotary shaft being located within the shaft passage. At least one annular laterally translatable seal carrier is provided. First and second annular resilient sealing elements are supported in axially spaced relation by the annular seal carriers and have sealing relation with the rotary shaft. The seal housing and at least one seal carrier define a first pressure staging chamber exposed to the first annular resilient sealing element and a second pressure staging chamber located between and exposed to the first and second annular resilient sealing elements. A first fluid is circulated to the first pressure chamber at a pressure P1, and a second staging pressure fluid is circulated to the second pressure chamber at a fraction of pressure P1 to achieve pressure staging, cooling of the seals. Seal placement provides hydraulic force balancing of the annular seal carriers.

  3. Electrically insulating and sealing frame

    DOEpatents

    Guthrie, Robin J.

    1983-11-08

    A combination gas seal and electrical insulator having a closed frame shape interconnects a fuel cell stack and a reactant gas plenum of a fuel cell generator. The frame can be of rectangular shape including at least one slidable spline connection in each side to permit expansion or contraction consistent with that of the walls of the gas plenum and fuel cell stack. The slidable spline connections in the frame sides minimizes lateral movement between the frame side members and sealing material interposed between the frame and the fuel cell stack or between the frame and the reactant gas plenum.

  4. Air bearing vacuum seal assembly

    DOEpatents

    Booth, Rex

    1978-01-01

    An air bearing vacuum seal assembly capable of rotating at the speed of several thousand revolutions per minute using an air cushion to prevent the rotating and stationary parts from touching, and a two stage differential pumping arrangement to maintain the pressure gradient between the air cushion and the vacuum so that the leak rate into the vacuum is, for example, less than 1 .times. 10.sup.-4 Pa m.sup.3 /s. The air bearing vacuum seal has particular application for mounting rotating targets to an evacuated accelerator beam tube for bombardment of the targets with high-power charged particle beams in vacuum.

  5. Dual rotating shaft seal apparatus

    DOEpatents

    Griggs, J.E.; Newman, H.J.

    1983-06-16

    The report is directed to apparatus suitable for transferring torque and rotary motion through a wall in a manner which is essentially gas impermeable. The apparatus can be used for pressurizing, agitating, and mixing fluids and features two ferrofluidic, i.e., ferrometic seals. Each seal is disposed on one of two supported shafts and each shaft is operably connected at one end to a gear mechanism and at its other end to an adjustable coupling means which is to be connected to a rotatable shaft extending through a wall through which torque and rotary motion are to be transferred.

  6. High temperature sealed electrochemical cell

    SciTech Connect

    Valentin Chung, Brice Hoani; Burke, Paul J.; Sadoway, Donald R.

    2015-10-06

    A cell for high temperature electrochemical reactions is provided. The cell includes a container, at least a portion of the container acting as a first electrode. An extension tube has a first end and a second end, the extension tube coupled to the container at the second end forming a conduit from the container to said first end. A second electrode is positioned in the container and extends out of the container via the conduit. A seal is positioned proximate the first end of the extension tube, for sealing the cell.

  7. Water conservation in fasting northern elephant seals (Mirounga angustirostris).

    PubMed

    Lester, Christopher W; Costa, Daniel P

    2006-11-01

    Prolonged terrestrial fasting is a key element in the life history of elephant seals. While on land seals typically fast without access to fresh water, and thus must maintain positive water balance by reductions in water loss such that they can subsist primarily on metabolic water production (MWP). The terrestrial apnea demonstrated by seals may reduce respiratory evaporative water loss (REWL) to levels that allow seals to make a net gain of water from MWP. We empirically measured REWL in 13 fasting northern elephant seal pups and determined the effects on water conservation of a breathing mode that incorporates a regular pattern of apneas, of > or =1 min in duration, followed by eupneic recovery, compared with a breathing mode with no apneas longer than 20 s and resembling typical breathing patterns in other mammals (normative breathing). Overall REWL fell 41% from 0.075+/-0.013 g min(-1) (mean +/- s.d.) during normative breathing to 0.044+/-0.006 g min(-1) during apneic breathing. The decline in REWL is attributed to a decrease in overall ventilation rate, made possible by a decline in metabolic rate along with an increase in oxygen extraction that would occur during apneic breathing. Data on the range of ambient humidity conditions at the local breeding site were collected and used to bound the range of environmental conditions used in laboratory measurements. Our data showed that the observed variations in ambient humidity had no significant effect on REWL. A combination of apneic breathing and the complex nasal turbinates allows fasting elephant seals to reduce REWL well below the rate of MWP so that they can maintain water balance during the fast.

  8. Experimental research on sealing of boreholes, shafts and ramps in welded tuff

    SciTech Connect

    Fuenkajorn, K.

    1996-04-01

    Laboratory and in-situ experiments have been conducted to determine the mechanical and hydraulic performance of cement borehole seals in densely welded Apache Leap tuff. Test results indicate that under saturated conditions, commercial expansive cement can provide good bond strength and adequate hydraulic performance for borehole seal under changing stress conditions. The cement seal should be installed at the intact portion of the opening, and should have a length-to-diameter ratio greater than four. Drying increases borehole plug permeability and decreases mechanical and hydraulic bonds at the plug-rock interface. In-situ testing indicates that installation procedure may significantly affect the cement plug performance.

  9. Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation.

    PubMed

    Weisenberger, Daniel J; Velicescu, Mihaela; Cheng, Jonathan C; Gonzales, Felicidad A; Liang, Gangning; Jones, Peter A

    2004-01-01

    Several alternatively spliced variants of DNA methyltransferase (DNMT) 3b have been described. Here, we identified new murine Dnmt3b mRNA isoforms and found that mouse embryonic stem (ES) cells expressed only Dnmt3b transcripts that contained exons 10 and 11, whereas the Dnmt3b transcripts in somatic cells lacked these exons, suggesting that this region is important for embryonic development. DNMT3b2 and 3b3 were the major isoforms expressed in human cell lines and the mRNA levels of these isoforms closely correlated with their protein levels. Although DNMT3b3 may be catalytically inactive, it still may be biologically important because D4Z4 and satellites 2 and 3 repeat sequences, all known DNMT3b target sequences, were methylated in cells that predominantly expressed DNMT3b3. Treatment of cells with the mechanism-based inhibitor 5-aza-2'-deoxycytidine (5-Aza-CdR) caused a complete depletion of DNMT1, 3a, 3b1, and 3b2 proteins. Human DNMT3b3 and the murine Dnmt3b3-like isoform, Dnmt3b6, were also depleted although less efficiently, suggesting that DNMT3b3 also may be capable of DNA binding. Moreover, de novo methylation of D4Z4 in T24 cancer cells after 5-Aza-CdR treatment only occurred when DNMT3b3 was expressed, reinforcing its role as a contributing factor of DNA methylation. The expression of either DNMT3b2 or 3b3, however, was not sufficient to explain the abnormal methylation of DNMT3b target sequences in human cancers, which may therefore be dependent on factors that affect DNMT3b targeting. Methylation analyses of immunodeficiency, chromosomal instabilities, and facial abnormalities cells revealed that an Alu repeat sequence was highly methylated, suggesting that Alu sequences are not DNMT3b targets.

  10. Sealing Assembly for Sealing a Port and the Like

    NASA Technical Reports Server (NTRS)

    Haas, Jon W. (Inventor); Haupt, Charles W. (Inventor)

    2000-01-01

    The sealing assembly for a port of a valve or the like is disclosed. In detail, the sealing assembly includes the port having a circular shaped end with a circular shaped knife-edge thereon. The sealing assembly further includes a hollow cap having a closed first end with an aperture therethrough and an open second end. The cap further includes internal threads adapted to mate with the external threads of the port. A gasket is mounted within the cap having flat first and second principle sides and made of a deformable metal, the first principle side of the gasket for mounting against the circular shaped knife edge of the port. A plunger having a circular shaped disc portion is adapted to fit within the hollow cap and is engagable with the first principle surface of the gasket and includes a shaft portion extending out of the aperture. The cap and shaft of the plunger include external wrenching flats. Thus when the cap is screwed onto the port and the plunger is prevented from rotating by a wrench mounted on the wrenching flats of the shaft portion of the plunger, the gasket is forced into engagement with the knife edge in pure compression and no rotation of the gasket occurs causing the knife edge to locally deform the gasket sealing of the port.

  11. Believing Your Eyes: Strengthening the Reliability of Tags and Seals

    SciTech Connect

    Brim, Cornelia P.; Denlinger, Laura S.

    2013-07-01

    NNSA’s Office of Nonproliferation and International Security (NIS) is working together with scientific experts at the DOE national laboratories to develop the tools needed to safeguard and secure nuclear material from diversion, theft, and sabotage--tasks critical to support future arms control treaties that may involve the new challenge of monitoring nuclear weapons dismantlement. Use of optically stimulated luminescent material is one method to enhance the security and robustness of existing tamper indicating devices such as tags and seals.

  12. 50 CFR Table 3b to Part 680 - Crab Disposition or Product Codes

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 13 2012-10-01 2012-10-01 false Crab Disposition or Product Codes 3b Table 3b to Part 680 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND... ZONE OFF ALASKA Pt. 680, Table 3b Table 3b to Part 680—Crab Disposition or Product Codes...

  13. 50 CFR Table 3b to Part 680 - Crab Disposition or Product Codes

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 50 Wildlife and Fisheries 9 2010-10-01 2010-10-01 false Crab Disposition or Product Codes 3b Table 3b to Part 680 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND... ZONE OFF ALASKA Pt. 680, Table 3b Table 3b to Part 680—Crab Disposition or Product Codes...

  14. 50 CFR Table 3b to Part 680 - Crab Disposition or Product Codes

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 11 2011-10-01 2011-10-01 false Crab Disposition or Product Codes 3b Table 3b to Part 680 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND... ZONE OFF ALASKA Pt. 680, Table 3b Table 3b to Part 680—Crab Disposition or Product Codes...

  15. 50 CFR Table 3b to Part 680 - Crab Disposition or Product Codes

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 13 2014-10-01 2014-10-01 false Crab Disposition or Product Codes 3b Table 3b to Part 680 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND... ZONE OFF ALASKA Pt. 680, Table 3b Table 3b to Part 680—Crab Disposition or Product Codes...

  16. 50 CFR Table 3b to Part 680 - Crab Disposition or Product Codes

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 50 Wildlife and Fisheries 13 2013-10-01 2013-10-01 false Crab Disposition or Product Codes 3b Table 3b to Part 680 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND... ZONE OFF ALASKA Pt. 680, Table 3b Table 3b to Part 680—Crab Disposition or Product Codes...

  17. High temperature seal for large structural movements

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M. (Inventor); Dunlap, Jr., Patrick H. (Inventor)

    2004-01-01

    A high temperature sealing system is operative to seal an interface between adjacent hot structures and to minimize parasitic flow between such structures that move relative to one another in-plane or out-of-plane. The sealing system may be used to seal thrust-directing ramp structures of a reusable launch vehicle and includes a channel and a plurality of movable segmented sealing elements. Adjacent ramp structures include edge walls which extend within the channel. The sealing elements are positioned along the sides of the channel and are biased to engage with the inner surfaces of the ramp structures. The segmented sealing elements are movable to correspond to the contour of the thrust-directing ramp structures. The sealing system is operative to prevent high temperature thrust gases that flow along the ramp structures from infiltrating into the interior of the vehicle.

  18. Knife-edge seal for vacuum bagging

    NASA Technical Reports Server (NTRS)

    Rauschl, J. A.

    1980-01-01

    Cam actuated clamps pinch bagging material between long knife edge (mounted to clamps) and high temperature rubber cushion bonded to baseplate. No adhesive, tape, or sealing groove is needed to seal edge of bagging sheet against base plate.

  19. Seal Apparatus and Methods to Manufacture Thereof

    NASA Technical Reports Server (NTRS)

    Richard, James A. (Inventor)

    2013-01-01

    In some implementations, apparatus and methods are provided through which a dynamic cryogenic seal is manufactured. In some implementations, the seal includes a retainer and a spring-seal assembly, the assembly being comprised of a main spring housing and fluorine-containing polymer seals. In some implementations, either a radial seal, or an axial (or "piston seal") is provided. In some implementations, methods of manufacturing the dynamic cryogenic seal are also provided. In some implementations, the methods include assembling the components while either heated or cooled, taking advantage of thermal expansion and contraction, such that there is a strong interference fit between the components at room temperature. In some implementations, this process ensures that the weaker fluorine-containing polymer seal is forced to expand and contract with the stronger retainer and spring and is under constant preload. In some implementations, the fluorine-containing polymer is therefore fluidized and retained, and can not lift off.

  20. Overview of LIDS Docking Seals Development

    NASA Technical Reports Server (NTRS)

    Dunlap, Patrick; Steinetz, Bruce; Daniels, Christopher

    2009-01-01

    GRC is supporting JSC by developing LIDS main interface seals Seal development and testing is occurring at both sub-scale and full-scale levels: a) Small-scale tests performed to define seal materials and evaluate exposure to space environments. b) Medium-scale testing: 1) Permits evaluation of candidate seal designs at faster pace than for full-scale seals. 2) Leak rates and loads can be scaled up to full-scale for indication of seal performance. c) Full-scale test rigs used for seal development and flight qualification tests and to assess on-orbit anomalies if needed. GRC responsible for delivering flight hardware seals to JSC approx.2013 for integration into LIDS flight units.

  1. Field testing of the Cobra Seal System

    SciTech Connect

    Yellin, E.; Vodrazka, P. ); Ystesund, K.; Drayer, D. )

    1990-01-01

    The Cobra Seal System consists of a passive fiber optic seal and verification equipment which have been modified to take advantage of current technology. The seal permits on-site verification without requiring replacement of the seal. The modifications to the original Cobra Seal System extended the maximum fiber optic cable length from 1 meter to 10 meters. This improvement allowed the Cobra Seal to be considered for application on dry irradiated fuel storage canisters at two Canadian facilities. These canisters are located in an exterior environment exposed to extreme weather conditions. This paper describe the application of the Cobra Seal to these canisters, a housing for the protection of the Cobra Seal body from the environment, and some preliminary results of the IAEA field tests. 4 refs.

  2. Film riding seals for rotary machines

    DOEpatents

    Bidkar, Rahul Anil; Sarawate, Neelesh Nandkumar; Wolfe, Christopher Edward; Ruggiero, Eric John; Raj Mohan, Vivek Raja

    2017-03-07

    A seal assembly for a rotary machine is provided. The seal assembly includes multiple sealing device segments disposed circumferentially intermediate to a stationary housing and a rotor. Each of the segments includes a shoe plate with a forward-shoe section and an aft-shoe section having one or more labyrinth teeth therebetween facing the rotor. The sealing device includes a stator interface element having a groove or slot for allowing disposal of a spline seal for preventing segment leakages. The sealing device segment also includes multiple bellow springs or flexures connected to the shoe plate and to the stator interface element. Further, the sealing device segments include a secondary seal integrated with the stator interface element at one end and positioned about the multiple bellow springs or flexures and the shoe plate at the other end.

  3. Compensating For Shrinkage In A Cryogenic Seal

    NASA Technical Reports Server (NTRS)

    Hill, Arnold E.

    1993-01-01

    Proposed design for seals in liquid-hydrogen plumbing eliminates leaks caused by contraction of seals at low operating temperature. Each seal consists of rubber, polytetrafluorethylene, or lead O-ring including hollow core filled with water. At temperature of liquid hydrogen, anomalous expansion of water keeps seal gland filled and leaktight despite shrinkage of surrounding O-ring material. Design also used in systems using cryogenic fluids other than liquid hydrogen.

  4. Dynamic Tester For Rotor Seals And Bearings

    NASA Technical Reports Server (NTRS)

    Von Pragenau, George L.

    1991-01-01

    Simplified apparatus measures performance under vibration. Measures some of dynamic parameters of rotor seals and bearings. Tests damping seals, damping bearings, conventional seals, and conventional bearings. Used with variety of pumped liquids, from water to liquid oxygen or hydrogen. Designed to test bearings and seals of turbopumps, tester rotates shaft at high speed while liquid flows much as it would in real turbopump. Also measures deflections of components.

  5. Non Destructive Seal Testing Polymeric Tray

    DTIC Science & Technology

    2006-10-01

    for 2 hours, the outside of the container seal is observed with a UV light to detect if the dye had penetrated through the seal. The dye test has been...electricity was 1micron (µm) in diameter and verified using transmitted light and a 1000-power microscope (Arndt et al., 1995). 3.1.8 Scanning...transmittance of heat energy (infrared light ) in a package or seal. Infrared light may be absorbed, transmitted, and emitted by a package or a seal

  6. Multi-layer seal for electrochemical devices

    DOEpatents

    Chou, Yeong-Shyung [Richland, WA; Meinhardt, Kerry D [Kennewick, WA; Stevenson, Jeffry W [Richland, WA

    2010-11-16

    Multi-layer seals are provided that find advantageous use for reducing leakage of gases between adjacent components of electrochemical devices. Multi-layer seals of the invention include a gasket body defining first and second opposing surfaces and a compliant interlayer positioned adjacent each of the first and second surfaces. Also provided are methods for making and using the multi-layer seals, and electrochemical devices including said seals.

  7. Multi-layer seal for electrochemical devices

    DOEpatents

    Chou, Yeong-Shyung [Richland, WA; Meinhardt, Kerry D [Kennewick, WA; Stevenson, Jeffry W [Richland, WA

    2010-09-14

    Multi-layer seals are provided that find advantageous use for reducing leakage of gases between adjacent components of electrochemical devices. Multi-layer seals of the invention include a gasket body defining first and second opposing surfaces and a compliant interlayer positioned adjacent each of the first and second surfaces. Also provided are methods for making and using the multi-layer seals, and electrochemical devices including said seals.

  8. Sealing apparatus utilizing a conformable member

    DOEpatents

    Neef, William S.; Lambert, Donald R.

    1988-01-01

    Sealing apparatus and method, comprising first and second surfaces or membranes, at least one of which surfaces is deformable, placed in proximity to one another. Urging means cause these surfaces to contact one another in a manner such that the deformable surface "deforms" to conform to the geometry of the other surface, thereby creating a seal. The seal is capable of undergoing multiple cycles of sealing and unsealing.

  9. Structural and functional partitioning of bread wheat chromosome 3B.

    PubMed

    Choulet, Frédéric; Alberti, Adriana; Theil, Sébastien; Glover, Natasha; Barbe, Valérie; Daron, Josquin; Pingault, Lise; Sourdille, Pierre; Couloux, Arnaud; Paux, Etienne; Leroy, Philippe; Mangenot, Sophie; Guilhot, Nicolas; Le Gouis, Jacques; Balfourier, Francois; Alaux, Michael; Jamilloux, Véronique; Poulain, Julie; Durand, Céline; Bellec, Arnaud; Gaspin, Christine; Safar, Jan; Dolezel, Jaroslav; Rogers, Jane; Vandepoele, Klaas; Aury, Jean-Marc; Mayer, Klaus; Berges, Hélène; Quesneville, Hadi; Wincker, Patrick; Feuillet, Catherine

    2014-07-18

    We produced a reference sequence of the 1-gigabase chromosome 3B of hexaploid bread wheat. By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85% of transposable elements. The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination. Comparative analyses indicated high wheat-specific inter- and intrachromosomal gene duplication activities that are potential sources of variability for adaption. In addition to providing a better understanding of the organization, function, and evolution of a large and polyploid genome, the availability of a high-quality sequence anchored to genetic maps will accelerate the identification of genes underlying important agronomic traits.

  10. Compressor seal rub energetics study

    NASA Technical Reports Server (NTRS)

    Laverty, W. F.

    1978-01-01

    The rub mechanics of compressor abradable blade tip seals at simulated engine conditions were investigated. Twelve statistically planned, instrumented rub tests were conducted with titanium blades and Feltmetal fibermetal rubstrips. The tests were conducted with single stationary blades rubbing against seal material bonded to rotating test disks. The instantaneous rub torque, speed, incursion rate and blade temperatures were continuously measured and recorded. Basic rub parameters (incursion rate, rub depth, abradable density, blade thickness and rub velocity) were varied to determine the effects on rub energy and heat split between the blade, rubstrip surface and rub debris. The test data was reduced, energies were determined and statistical analyses were completed to determine the primary and interactive effects. Wear surface morphology, profile measurements and metallographic analysis were used to determine wear, glazing, melting and material transfer. The rub energies for these tests were most significantly affected by the incursion rate while rub velocity and blade thickness were of secondary importance. The ratios of blade wear to seal wear were representative of those experienced in engine operation of these seal system materials.

  11. Pre-sealing risk analysis

    SciTech Connect

    Ensminger, D.A.; Hough, M.E.; Oston, S.G.

    1980-01-07

    This report describes studies of accidents involving high-level radioactive waste before sealing the waste into a repository. The report summarizes work done in this area during Fiscal Year 1978 and supplements previous work. Models of accident probability, severity, and consequences are refined and extended.

  12. SEAL /Subnetwork Enumeration And Listing/

    NASA Technical Reports Server (NTRS)

    Happ, W. W.; Mc Intosh, F. J.

    1968-01-01

    SEAL /Subnetwork Enumeration And Listing/ computer program uses combinatorial techniques to generate all of the nonredundant subnetwork configurations derivable from an asymmetrical network or device. This is accomplished by a systematic shorting and opening of accessible terminals to obtain the desired allowable configurations.

  13. FLUID PURIFIER AND SEALING VALVE

    DOEpatents

    Swanton, W.F.

    1962-04-24

    An improved cold trap designed to condense vapors and collect foreign particles in a flowing fluid is described. In the arrangement, a valve is provided to prevent flow reversal in case of pump failure and to act as a sealing valve. Provision is made for reducing the temperature of the fluid being processed, including a pre-cooling stage. (AEC)

  14. Hermetic Edge Seals for Photovoltaic Modules

    NASA Technical Reports Server (NTRS)

    Nowlan, M. J.

    1986-01-01

    Corrosive atmospheric agents excluded to prolong cell life. Combination of two sealing techniques makes possible to protect solar cells from water vapor, oxygen, and other corrosive atmospheric constituents. Using three-step process, glass-to-metal hermetic seal formed around edge of solar-cell module. Elastomer seals used previously not as effective because they are permeable to water vapor and atmospheric gases.

  15. Improved Seals for High Temperature Airframe Applications

    NASA Technical Reports Server (NTRS)

    DeMange, Jeffrey J.; Dunlap, Patrick H.; Steinetz, Bruce M.

    2006-01-01

    Current thermal barrier seals, such as those used on the Space Shuttle, are insufficient to fully meet the demands of future hypersonic vehicles and reentry spacecraft. Previous investigations have demonstrated limited usage temperatures, as evidenced by a decreased ability to maintain sealing effectiveness at high temperatures (i.e., inadequate resiliency). In order to improve resiliency at elevated temperatures, Rene 41 (Allvac) was substituted for Inconel X-750 (Special Metals Corp.) as the spring tube material in the existing seal design. A seal construction incorporating the Rene 41 spring tube was fabricated and tested against the baseline Inconel X-750 spring tube seal. Although resiliency improvements were not as dramatic as in previous tests with the spring tubes alone, seals incorporating the Rene 41 spring tube exhibited an average 20 percent resiliency enhancement up to 1750 F when compared to seals containing the Inconel spring tube. In addition, the seals with the Rene 41 spring tubes showed less reduction in resiliency as temperatures increased above 1200 F. Results also indicated the Saffil (Saffil Ltd.) insulation in the core of the seal contributed more to resiliency than previously thought. Leakage data did not demonstrate an improvement with the seal containing the Rene 41 spring tube. However, based upon resiliency results, one could reasonably expect the Rene 41 version of the seal to track gap openings over a wider range. Therefore it would exhibit lower leakage than the Inconel X-750 version as the seal gap opens during a typical mission.

  16. Piston rod seal for a Stirling engine

    SciTech Connect

    Shapiro, Wilbur

    1984-01-01

    In a piston rod seal for a Stirling engine, a hydrostatic bearing and differential pressure regulating valve are utilized to provide for a low pressure differential across a rubbing seal between the hydrogen and oil so as to reduce wear on the seal.

  17. A Sample Return Container with Hermetic Seal

    NASA Technical Reports Server (NTRS)

    Kong, Kin Yuen; Rafeek, Shaheed; Sadick, Shazad; Porter, Christopher C.

    2000-01-01

    A sample return container is being developed by Honeybee Robotics to receive samples from a derivative of the Champollion/ST4 Sample Acquisition and Transfer Mechanism or other samplers and then hermetically seal samples for a sample return mission. The container is enclosed in a phase change material (PCM) chamber to prevent phase change during return and re-entry to earth. This container is designed to operate passively with no motors and actuators. Using the sampler's featured drill tip for interfacing, transfer-ring and sealing samples, the container consumes no electrical power and therefore minimizes sample temperature change. The circular container houses a few isolated canisters, which will be sealed individually for samples acquired from different sites or depths. The drill based sampler indexes each canister to the sample transfer position, below the index interface for sample transfer. After sample transfer is completed, the sampler indexes a seal carrier, which lines up seals with the openings of the canisters. The sampler moves to the sealing interface and seals the sample canisters one by one. The sealing interface can be designed to work with C-seals, knife edge seals and cup seals. Again, the sampler provides all sealing actuation. This sample return container and co-engineered sample acquisition system are being developed by Honeybee Robotics in collaboration with the JPL Exploration Technology program.

  18. Annular Pressure Seals and Hydrostatic Bearings

    DTIC Science & Technology

    2006-11-01

    affecting the rotordynamics of liquid turbopumps, in particular those handling large density fluids. Highlights on the bulk-flow analysis of annular seals... rotordynamic stability. Hydrostatic bearings rely on external fluid pressurization to generate load support and large centering stiffnesses, even in...SEALS IN PUMP APPLICATIONS Seal rotordynamic characteristic have a primary influence on the stability response of high-performance turbomachinery [1

  19. 1 CFR 18.8 - Seal.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 1 General Provisions 1 2010-01-01 2010-01-01 false Seal. 18.8 Section 18.8 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER PREPARATION, TRANSMITTAL, AND PROCESSING OF DOCUMENTS PREPARATION AND TRANSMITTAL OF DOCUMENTS GENERALLY § 18.8 Seal. Use of a seal on an original document...

  20. 19 CFR 201.0 - Seal.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 3 2013-04-01 2013-04-01 false Seal. 201.0 Section 201.0 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES OF GENERAL APPLICATION § 201.0 Seal. (a) Pursuant to... Trade Commission has adopted an official seal, the depiction of which follows: EC05OC91.026 (b)...

  1. 28 CFR 0.146 - Seals.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 28 Judicial Administration 1 2011-07-01 2011-07-01 false Seals. 0.146 Section 0.146 Judicial... Personnel and Certain Administrative Matters § 0.146 Seals. The Director of the Federal Bureau of... the U.S. Marshals Service shall each have custody of the seal pertaining to his...

  2. 19 CFR 201.0 - Seal.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 3 2014-04-01 2014-04-01 false Seal. 201.0 Section 201.0 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES OF GENERAL APPLICATION § 201.0 Seal. (a) Pursuant to... Trade Commission has adopted an official seal, the depiction of which follows: EC05OC91.026 (b)...

  3. 19 CFR 201.0 - Seal.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 3 2012-04-01 2012-04-01 false Seal. 201.0 Section 201.0 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES OF GENERAL APPLICATION § 201.0 Seal. (a) Pursuant to... Trade Commission has adopted an official seal, the depiction of which follows: EC05OC91.026 (b)...

  4. 40 CFR 147.3103 - Fluid seals.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 24 2012-07-01 2012-07-01 false Fluid seals. 147.3103 Section 147.3103... Tribes § 147.3103 Fluid seals. Notwithstanding §§ 144.28(f)(2) and 146.12(c) of this chapter, owners and operators shall not use a fluid seal as an alternative to a packer....

  5. 14 CFR 187.7 - Copies; seal.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Copies; seal. 187.7 Section 187.7... REGULATIONS FEES § 187.7 Copies; seal. The fees for furnishing photostatic or similar copies of documents and for affixation of the seal for a certification or validation are the same as those provided in...

  6. 28 CFR 0.146 - Seals.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 1 2012-07-01 2012-07-01 false Seals. 0.146 Section 0.146 Judicial... Personnel and Certain Administrative Matters § 0.146 Seals. The Director of the Federal Bureau of... the U.S. Marshals Service shall each have custody of the seal pertaining to his...

  7. 29 CFR 4002.11 - Seal.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 9 2014-07-01 2014-07-01 false Seal. 4002.11 Section 4002.11 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION GENERAL BYLAWS OF THE PENSION BENEFIT GUARANTY CORPORATION § 4002.11 Seal. The seal of the Corporation shall be in such form as may be approved from time...

  8. 40 CFR 147.3103 - Fluid seals.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 23 2014-07-01 2014-07-01 false Fluid seals. 147.3103 Section 147.3103... Tribes § 147.3103 Fluid seals. Notwithstanding §§ 144.28(f)(2) and 146.12(c) of this chapter, owners and operators shall not use a fluid seal as an alternative to a packer....

  9. 19 CFR 201.0 - Seal.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 3 2010-04-01 2010-04-01 false Seal. 201.0 Section 201.0 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES OF GENERAL APPLICATION § 201.0 Seal. (a) Pursuant to... Trade Commission has adopted an official seal, the depiction of which follows: EC05OC91.026 (b)...

  10. 28 CFR 0.146 - Seals.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 28 Judicial Administration 1 2014-07-01 2014-07-01 false Seals. 0.146 Section 0.146 Judicial... Personnel and Certain Administrative Matters § 0.146 Seals. The Director of the Federal Bureau of... the U.S. Marshals Service shall each have custody of the seal pertaining to his...

  11. 19 CFR 201.0 - Seal.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 3 2011-04-01 2011-04-01 false Seal. 201.0 Section 201.0 Customs Duties UNITED STATES INTERNATIONAL TRADE COMMISSION GENERAL RULES OF GENERAL APPLICATION § 201.0 Seal. (a) Pursuant to... Trade Commission has adopted an official seal, the depiction of which follows: EC05OC91.026 (b)...

  12. 29 CFR 4002.11 - Seal.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 9 2012-07-01 2012-07-01 false Seal. 4002.11 Section 4002.11 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION GENERAL BYLAWS OF THE PENSION BENEFIT GUARANTY CORPORATION § 4002.11 Seal. The seal of the Corporation shall be in such form as may be approved from time...

  13. 14 CFR 187.7 - Copies; seal.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Copies; seal. 187.7 Section 187.7... REGULATIONS FEES § 187.7 Copies; seal. The fees for furnishing photostatic or similar copies of documents and for affixation of the seal for a certification or validation are the same as those provided in...

  14. 1 CFR 18.8 - Seal.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 1 General Provisions 1 2013-01-01 2012-01-01 true Seal. 18.8 Section 18.8 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER PREPARATION, TRANSMITTAL, AND PROCESSING OF DOCUMENTS PREPARATION AND TRANSMITTAL OF DOCUMENTS GENERALLY § 18.8 Seal. Use of a seal on an original document...

  15. 14 CFR 187.7 - Copies; seal.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Copies; seal. 187.7 Section 187.7... REGULATIONS FEES § 187.7 Copies; seal. The fees for furnishing photostatic or similar copies of documents and for affixation of the seal for a certification or validation are the same as those provided in...

  16. 29 CFR 4002.11 - Seal.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 9 2011-07-01 2011-07-01 false Seal. 4002.11 Section 4002.11 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION GENERAL BYLAWS OF THE PENSION BENEFIT GUARANTY CORPORATION § 4002.11 Seal. The seal of the Corporation shall be in such form as may be approved from time...

  17. 29 CFR 4002.11 - Seal.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 9 2013-07-01 2013-07-01 false Seal. 4002.11 Section 4002.11 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION GENERAL BYLAWS OF THE PENSION BENEFIT GUARANTY CORPORATION § 4002.11 Seal. The seal of the Corporation shall be in such form as may be approved from time...

  18. 1 CFR 18.8 - Seal.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 1 General Provisions 1 2012-01-01 2012-01-01 false Seal. 18.8 Section 18.8 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER PREPARATION, TRANSMITTAL, AND PROCESSING OF DOCUMENTS PREPARATION AND TRANSMITTAL OF DOCUMENTS GENERALLY § 18.8 Seal. Use of a seal on an original document...

  19. 14 CFR 187.7 - Copies; seal.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Copies; seal. 187.7 Section 187.7... REGULATIONS FEES § 187.7 Copies; seal. The fees for furnishing photostatic or similar copies of documents and for affixation of the seal for a certification or validation are the same as those provided in...

  20. 1 CFR 18.8 - Seal.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 1 General Provisions 1 2011-01-01 2011-01-01 false Seal. 18.8 Section 18.8 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER PREPARATION, TRANSMITTAL, AND PROCESSING OF DOCUMENTS PREPARATION AND TRANSMITTAL OF DOCUMENTS GENERALLY § 18.8 Seal. Use of a seal on an original document...

  1. 40 CFR 147.3103 - Fluid seals.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 24 2013-07-01 2013-07-01 false Fluid seals. 147.3103 Section 147.3103... Tribes § 147.3103 Fluid seals. Notwithstanding §§ 144.28(f)(2) and 146.12(c) of this chapter, owners and operators shall not use a fluid seal as an alternative to a packer....

  2. 29 CFR 4002.11 - Seal.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Seal. 4002.11 Section 4002.11 Labor Regulations Relating to Labor (Continued) PENSION BENEFIT GUARANTY CORPORATION GENERAL BYLAWS OF THE PENSION BENEFIT GUARANTY CORPORATION § 4002.11 Seal. The seal of the Corporation shall be in such form as may be approved from time...

  3. 14 CFR 187.7 - Copies; seal.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Copies; seal. 187.7 Section 187.7... REGULATIONS FEES § 187.7 Copies; seal. The fees for furnishing photostatic or similar copies of documents and for affixation of the seal for a certification or validation are the same as those provided in...

  4. 1 CFR 18.8 - Seal.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 1 General Provisions 1 2014-01-01 2012-01-01 true Seal. 18.8 Section 18.8 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL REGISTER PREPARATION, TRANSMITTAL, AND PROCESSING OF DOCUMENTS PREPARATION AND TRANSMITTAL OF DOCUMENTS GENERALLY § 18.8 Seal. Use of a seal on an original document...

  5. Rubber valve seal with tough skin

    NASA Technical Reports Server (NTRS)

    Martin, J. W.

    1979-01-01

    Curing technique for producing variable viscosity seal has hard sealing surface supported by softer rubber. Valve seal is clamped between two jaws for curing with hotter jaw at temperature of approximately 350 F and lower at room temperature. Result is durable tight valve-seat.

  6. Advanced bristle seals for gas turbine engines

    NASA Astrophysics Data System (ADS)

    Cabe, Jerry L.

    1993-01-01

    A seven month proof-of-concept program was conducted for an advanced bristle seal, called a bush seal, for use in gas turbine engines. This program was performed as a Small Business Innovation Research (SBIR) Phase 1 project. Bush seal specimen and a full ring bush seal were designed, evaluated, and manufactured for testing. An analytical study of the potential of the bush seal relative to a labyrinth seal was conducted. Static and dynamic testing of the bush seal was performed to determine the behavior of the bristles under pressurization and during contact with a rotating labyrinth tooth. Stable behavior of the bristle elements was observed during static pressurization of a full ring bush seal. The dynamic testing of various configurations of bush seal against a rotating labyrinth tooth showed minimal wear of the bristles relative to a conventional labyrinth seal. The development and application of the bush seal concept to gas turbine engines has the potential of improving the engine's performance while decreasing the degradation of the seal performance over time.

  7. High-Temperature, Bellows Hybrid Seal

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M. (Inventor); Sirocky, Paul J. (Inventor)

    1994-01-01

    A high-temperature hybrid seal is constructed of multiple elements to meet the many demands placed on the seal. The primary elements are: a central high-temperature bellows, a braided ceramic sheath covering the bellows, an outer abrasion resistant sheath covering the ceramic sheath, and a structurally-sound seal-end termination.

  8. Aerodynamic seal assemblies for turbo-machinery

    DOEpatents

    Bidkar, Rahul Anil; Wolfe, Christopher; Fang, Biao

    2015-09-29

    The present application provides an aerodynamic seal assembly for use with a turbo-machine. The aerodynamic seal assembly may include a number of springs, a shoe connected to the springs, and a secondary seal positioned about the springs and the shoe.

  9. Dampers for Stationary Labyrinth Seals

    NASA Technical Reports Server (NTRS)

    El-Aini, Yehia; Mitchell, William; Roberts, Lawrence; Montgomery, Stuart; Davis, Gary

    2011-01-01

    Vibration dampers have been invented that are incorporated as components within the stationary labyrinth seal assembly. These dampers are intended to supplement other vibration-suppressing features of labyrinth seals in order to reduce the incidence of high-cycle-fatigue failures, which have been known to occur in the severe vibratory environments of jet engines and turbopumps in which labyrinth seals are typically used. A vibration damper of this type includes several leaf springs and/or a number of metallic particles (shot) all held in an annular seal cavity by a retaining ring. The leaf springs are made of a spring steel alloy chosen, in conjunction with design parameters, to maintain sufficient preload to ensure effectiveness of damping at desired operating temperatures. The cavity is vented via a small radial gap between the retaining ring and seal housing. The damping mechanism is complex. In the case of leaf springs, the mechanism is mainly friction in the slippage between the seal housing and individual dampers. In the case of a damper that contains shot, the damping mechanism includes contributions from friction between individual particles, friction between particles and cavity walls, and dissipation of kinetic energy of impact. The basic concept of particle/shot vibration dampers has been published previously; what is new here is the use of such dampers to suppress traveling-wave vibrations in labyrinth seals. Damping effectiveness depends on many parameters, including, but not limited to, coefficient of friction, mode shape, and frequency and amplitude of vibrational modes. In tests, preloads of the order of 6 to 15 lb (2.72 to 6.8 kilograms) per spring damper were demonstrated to provide adequate damping levels. Effectiveness of shot damping of vibrations having amplitudes from 20 to 200 times normal terrestrial gravitational acceleration (196 to 1,960 meters per square second) and frequencies up to 12 kHz was demonstrated for shot sizes from 0.032 to

  10. Sealing performance of a magnetic fluid seal for rotary blood pumps.

    PubMed

    Mitamura, Yoshinori; Takahashi, Sayaka; Kano, Kentaro; Okamoto, Eiji; Murabayashi, Shun; Nishimura, Ikuya; Higuchi, Taka-Aki

    2009-09-01

    A magnetic fluid (MF) for a rotary blood pump seal enables mechanical contact-free rotation of the shaft and, hence, has excellent durability. The performance of a MF seal, however, has been reported to decrease in liquids. We have developed a MF seal that has a "shield" mechanism and a new MF with a higher magnetization of 47.9 kA/m. The sealing performance of the MF seal installed in a rotary blood pump was studied. Under the condition of continuous flow, the MF seal remained in perfect condition against a pressure of 298 mm Hg (pump flow rate: 3.96 L/min). The seal was also perfect against a pressure of 170 mm Hg in a continuous flow of 3.9 L/min for 275 days. We have developed a MF seal that works in liquid against clinically used pressures. The MF seal is promising as a shaft seal for rotary blood pumps.

  11. WIPP shaft seal system parameters recommended to support compliance calculations

    SciTech Connect

    Hurtado, L.D.; Knowles, M.K.; Kelley, V.A.; Jones, T.L.; Ogintz, J.B.; Pfeifle, T.W.

    1997-12-01

    The US Department of Energy plans to dispose of transuranic waste at the Waste Isolation Pilot Plant (WIPP), which is sited in southeastern New Mexico. The WIPP disposal facility is located approximately 2,150 feet (650 m) below surface in the bedded halite of the Salado Formation. Prior to initiation of disposal activities, the Department of Energy must demonstrate that the WIPP will comply with all regulatory requirements. Applicable regulations require that contaminant releases from the WIPP remain below specified levels for a period of 10,000 years. To demonstrate that the WIPP will comply with these regulations, the Department of Energy has requested that Sandia National Laboratories develop and implement a comprehensive performance assessment of the WIPP repository for the regulatory period. This document presents the conceptual model of the shaft sealing system to be implemented in performance assessment calculations conducted in support of the Compliance Certification Application for the WIPP. The model was developed for use in repository-scale calculations and includes the seal system geometry and materials to be used in grid development as well as all parameters needed to describe the seal materials. These calculations predict the hydrologic behavior of the system. Hence conceptual model development is limited to those processes that could impact the fluid flow through the seal system.

  12. Modulation of Dnmt3b function in vitro by interactions with Dnmt3L, Dnmt3a and Dnmt3b splice variants.

    PubMed

    Van Emburgh, Beth O; Robertson, Keith D

    2011-07-01

    DNA methylation, an essential regulator of transcription and chromatin structure, is established and maintained by the coordinated action of three DNA methyltransferases: DNMT1, DNMT3A and DNMT3B, and the inactive accessory factor DNMT3L. Disruptions in DNMT3B function are linked to carcinogenesis and genetic disease. DNMT3B is also highly alternatively spliced in a tissue- and disease-specific manner. The impact of intra-DNMT3 interactions and alternative splicing on the function of DNMT3 family members remains unclear. In the present work, we focused on DNMT3B. Using a panel of in vitro assays, we examined the consequences of DNMT3B splicing and mutations on its ability to bind DNA, interact with itself and other DNMT3's, and methylate DNA. Our results show that, while the C-terminal catalytic domain is critical for most DNMT3B functions, parts of the N-terminal region, including the PWWP domain, are also important. Alternative splicing and domain deletions also influence DNMT3B's cellular localization. Furthermore, our data reveal the existence of extensive DNMT3B self-interactions that differentially impact on its activity. Finally, we show that catalytically inactive isoforms of DNMT3B are capable of modulating the activity of DNMT3A-DNMT3L complexes. Our studies therefore suggest that seemingly 'inactive' DNMT3B isoforms may influence genomic methylation patterns in vivo.

  13. RAMONA-3B calculations for Browns Ferry ATWS (Anticipated Transient Without Scram) study

    SciTech Connect

    Saha, P; Slovik, G C; Neymotin, L Y

    1987-02-01

    Several aspects of the Anticipated Transient Without Scram (ATWS) initiated by an inadvertent closure of all Main Steam Isolation Valves (MSIV) in a typical BWR/4 are analyzed in the report. The analysis is performed using the Brookhaven National Laboratory code, RAMONA-3B, which employs a three-dimensional neutron kinetics model coupled with a parallel-channel thermal hydraulics in representing a Boiling Water Reactor (BWR) Core. Four different transient scenarios have been investigated: (a) downcomer water level and reactor pressure control, (b) manual control rod insertion transient, (c) high pressure boil-off, and (d) recirculation pump trip failure. Results of these calculations should provide better understanding of mitigative effects of operator actions during ATWS, thus helping in the development of adequate Emergency Procedure Guidelines (EPG) required for the BWR plant safety. A few unresolved questions subject to future investigations are also discussed.

  14. Method for producing a fuel cell manifold seal

    DOEpatents

    Grevstad, Paul E.; Johnson, Carl K.; Mientek, Anthony P.

    1982-01-01

    A manifold-to-stack seal and sealing method for fuel cell stacks. This seal system solves the problem of maintaining a low leak rate manifold seal as the fuel cell stack undergoes compressive creep. The seal system eliminates the problem of the manifold-to-stack seal sliding against the rough stack surface as the stack becomes shorter because of cell creep, which relative motion destroys the seal. The seal system described herein utilizes a polymer seal frame firmly clamped between the manifold and the stack such that the seal frame moves with the stack. Thus, as the stack creeps, the seal frame creeps with it, and there is no sliding at the rough, tough to seal, stack-to-seal frame interface. Here the sliding is on a smooth easy to seal location between the seal frame and the manifold.

  15. FNDC3B promotes cell migration and tumor metastasis in hepatocellular carcinoma

    PubMed Central

    Lin, Chin-Hui; Lin, Yao-Wen; Chen, Ying-Chun; Liao, Chen-Chung; Jou, Yuh-Shan; Hsu, Ming-Ta; Chen, Chian-Feng

    2016-01-01

    Recurrence and metastasis are common in hepatocellular carcinoma (HCC) and correlate with poor prognosis. We investigated the role of fibronectin type III domain containing 3B (FNDC3B) in HCC metastasis. Overexpression of FNDC3B in HCC cell lines enhanced cell migration and invasion. On the other hand, knockdown of FNDC3B using short-hairpin RNA reduced tumor nodule formation in both intra- and extra-hepatic metastasis. High levels of FNDC3B were observed in metastatic HCCs and correlated with poor patient survival and shorter recurrence time. Mutagenesis and LC-MS/MS analyses showed that FNDC3B promotes cell migration by cooperating with annexin A2 (ANXA2). Furthermore, FNDC3B and ANXA2 expression correlated negatively with patient survival. Our results indicate that FNDC3B behaves like an oncogene by promoting cell migration. This suggests FNDC3B could serve as a biomarker and therapeutic target for HCC metastasis. PMID:27385217

  16. Dynamic analysis of noncontacting face seals

    NASA Technical Reports Server (NTRS)

    Etsion, I.

    1980-01-01

    The dynamic behavior of a noncontacting coned face seal is analyzed taking into account various design parameters and operating conditions. The primary seal ring motion is expressed by a set of nonlinear equations for three degrees of freedom. These equations, which are solved numerically, allow identification of two dimensionless groups of parameters that affect the seal dynamic behavior. Stability maps for various seals are presented. These maps contain a stable-to-unstable transition region in which the ring wobbles at half the shaft frequency. The effect of various parameters on seal stability is discussed and an empirical expression for critical stability is offered.

  17. Turbine Seal Research at NASA GRC

    NASA Technical Reports Server (NTRS)

    Proctor, Margaret P.; Steinetz, Bruce M.; Delgado, Irebert R.; Hendricks, Robert C.

    2011-01-01

    Low-leakage, long-life turbomachinery seals are important to both Space and Aeronautics Missions. (1) Increased payload capability (2) Decreased specific fuel consumption and emissions (3) Decreased direct operating costs. NASA GRC has a history of significant accomplishments and collaboration with industry and academia in seals research. NASA's unique, state-of-the-art High Temperature, High Speed Turbine Seal Test Facility is an asset to the U.S. Engine / Seal Community. Current focus is on developing experimentally validated compliant, non-contacting, high temperature seal designs, analysis, and design methodologies to enable commercialization.

  18. Energy conversion device with improved seal

    DOEpatents

    Miller, Gerald R.; Virkar, Anil V.

    1980-01-01

    An energy conversion device comprising an improved sealing member adapted to seal a cation-permeable casing to the remainder of the device. The sealing member comprises a metal substrate which (i) bears a nonconductive and corrosion resistant coating on the major surface to which said casing is sealed, and (ii) is corrugated so as to render it flexible, thereby allowing said member to move relative to said casing without cracking the seal therebetween. Corrugations may be circumferential, radial, or both radial and circumferential so as to form dimples. The corrugated member may be in form of a bellows or in a substantially flat form, such as a disc.

  19. Actively controlled shaft seals for aerospace applications

    NASA Technical Reports Server (NTRS)

    Salant, Richard F.

    1991-01-01

    The main objective is to determine the feasibility of utilizing controllable mechanical seals for aerospace applications. A potential application was selected as a demonstration case: the buffer gas seal in a LOX (liquid oxygen) turbopump. Currently, floating ring seals are used in this application. Their replacement with controllable mechanical seals would result in substantially reduced leakage rates. This would reduce the required amount of stored buffer gas, and therefore increase the vehicle payload. For such an application, a suitable controllable mechanical seal was designed and analyzed.

  20. Pump seal standard API 682 has deficiencies

    SciTech Connect

    Iyer, K.R.

    1997-08-01

    Struggling to meet ever-tightening, stringent emission regulations, hydrocarbon processing plants are evaluating all possible alternatives that can cut or eliminate fugitive releases. Nearly 70% of centrifugal pump maintenance is due to mechanical seal failures, a leading contributor to fugitive emissions. The new standard--API 682 was developed exclusively to set guidelines that dictate mechanical seal performance and specifications. API 682`s goal: define centrifugal pump seal-sealing systems performance and design criteria that will improve reliability, increase pump-seal service life and help control VOC emissions. The article reviews the pros and cons for API 682 from the pump-user`s viewpoint.

  1. Experimental rotordynamic coefficient results for honeycomb seals

    NASA Technical Reports Server (NTRS)

    Elrod, David A.; Childs, Dara W.

    1988-01-01

    Test results (leakage and rotordynamic coefficients) are presented for seven honeycomb-stator smooth-rotor seals. Tests were carried out with air at rotor speeds up to 16,000 cpm and supply pressures up to 8.2 bars. Test results for the seven seals are compared, and the most stable configuration is identified based on the whirl frequency ratio. Results from tests of a smooth-rotor/smooth-stator seal, a teeth-on-stator labyrinth seal, and the most stable honeycomb seal are compared.

  2. Resilient Flexible Pressure-Activated Seal

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M. (Inventor); Dunlap, Patrick H., Jr. (Inventor)

    2009-01-01

    A resilient, flexible, pressure-activated, high-temperature seal is adapted to be interposed between high and low pressure regions to provide sealing between opposing surfaces of adjacent relatively movable structures. The seal comprises at least one C-shaped sheet element. The C-shaped element design enables the seal to be pressure-activated to provide a radially outward biasing force, responsive to a seal-activating pressure differential acting across the seal thereby increasing resiliency. A centrally-located, resilient core structure provides load bearing and insulating properties. In an exemplary embodiment where at least two seal elements are used, each layer has a cutout slot pattern and the remaining strip material pattern. The slots provide flexibility to the seal, enabling the seal to be manually contoured to seal around corners and curves. The strip material of each layer covers the slots in each adjacent layer to minimize leakage through the slots. Attached barrier strips can block interface leakage between the seal and the opposing surfaces.

  3. Double angle seal forming lubricant film

    DOEpatents

    Ernst, William D.

    1984-01-01

    A lubricated piston rod seal which inhibits gas leaking from a high pressure chamber on one side of the seal to a low pressure chamber on the other side of the seal. A liquid is supplied to the surface of the piston rod on the low pressure side of the seal. This liquid acts as lubricant for the seal and provides cooling for the rod. The seal, which can be a plastic, elastomer or other material with low elastic modulus, is designed to positively pump lubricant through the piston rod/seal interface in both directions when the piston rod is reciprocating. The capacity of the seal to pump lubricant from the low pressure side to the high pressure side is less than its capacity to pump lubricant from the high pressure side to the low pressure side which ensures that there is zero net flow of lubricant to the high pressure side of the seal. The film of lubricant between the seal and the rod minimizes any sliding contact and prevents the leakage of gas. Under static conditions gas leakage is prevented by direct contact between the seal and the rod.

  4. Sealing glass ampoules with CO2 lasers.

    PubMed

    Jiao, Junke; Wang, Xinbing; Tang, Wenlong

    2008-12-10

    Glass ampoules were always sealed by melting in the presence of a flame to create closures. Some poisonous gases were generated in this sealing process that pollute the injection drug and are physically harmful. In this study, CO(2) lasers were proposed for sealing glass ampoules. Because of the clean noncontact sealing process with lasers, there was nearly no pollution of the injection drug. To study in detail the principle of this sealing process, a mathematical model was put forward, and the temperature and the thermal stress field around the ampoule's neck were calculated by ANSYS software. Through experimental study, 1 ml and 5 ml ampoules were sealed successfully by a dual-laser-beam method. The results show that a laser source is an ideal heat source for sealing glass ampoules.

  5. Rotary seal with improved film distribution

    DOEpatents

    Dietle, Lannie Laroy; Schroeder, John Erick

    2013-10-08

    The present invention is a generally circular rotary seal that establishes sealing between relatively rotatable machine components for lubricant retention and environmental exclusion, and incorporates seal geometry that interacts with the lubricant during relative rotation to distribute a lubricant film within the dynamic sealing interface. The features of a variable inlet size, a variable dynamic lip flank slope, and a reduction in the magnitude and circumferentially oriented portion of the lubricant side interfacial contact pressure zone at the narrowest part of the lip, individually or in combination thereof, serve to maximize interfacial lubrication in severe operating conditions, and also serve to minimize lubricant shear area, seal torque, seal volume, and wear, while ensuring retrofitability into the seal grooves of existing equipment.

  6. Rotary seal with improved film distribution

    DOEpatents

    Dietle, Lannie Laroy; Schroeder, John Erick

    2015-09-01

    The present invention is a generally circular rotary seal that establishes sealing between relatively rotatable machine components for lubricant retention and environmental exclusion, and incorporates seal geometry that interacts with the lubricant during relative rotation to distribute a lubricant film within the dynamic sealing interface. The features of a variable inlet size, a variable dynamic lip flank slope, and a reduction in the magnitude and circumferentially oriented portion of the lubricant side interfacial contact pressure zone at the narrowest part of the lip, individually or in combination thereof, serve to maximize interfacial lubrication in severe operating conditions, and also serve to minimize lubricant shear area, seal torque, seal volume, and wear, while ensuring retrofitability into the seal grooves of existing equipment.

  7. Shape memory alloy seals for geothermal applications

    SciTech Connect

    Not Available

    1985-09-15

    A shape memory radial seal was fabricated with a ''U'' cross section. Upon heating the seal recovered its original ''V'' shape and produced a high pressure seal. The sealing pressure which can be developed is approximately 41 MPa (60,000 psi), well in excess of the pressure which can be produced in conventional elastomeric seals. The low modulus martensite can conform readily to the sealing surface, and upon recovery produce a seal capable of high pressure fluid or gas confinement. The corrosion resistance of nickel-titanium in a broad range of aggressive fluids has been well established and, as such, there is little doubt that, had time permitted, a geothermal pump of flange fluid tried would have been successful.

  8. Development of helicopter transmission seals, task 2

    NASA Technical Reports Server (NTRS)

    Hayden, T. S.; Keller, C. H., Jr.

    1973-01-01

    High speed helicopter transmission seal concepts were designed, fabricated and tested. The concepts were a dual element split ring seal and a circumferential seal. The tests were performed in a rig using an actual input quill assembly. The test conditions were selected to simulate transmission operation and were 230 F oil temperature, and a sliding speed of 9400 ft/min. The split ring seal exhibited gross leakage and was considered unsatisfactory, while the circumferential seal leakage was less than 1 c.c./hour; this leakage is within acceptable limits. The circumferential seal wear was only to .0005 inches during a 100 hour run (40 starts and stops). During a 40 hour contamination test (mesh silica flour) the seal total wear was a maximum of .004 inches. This wear is considered acceptable.

  9. Seal dynamics on the Swedish west coast: Scenarios of competition as Baltic grey seal intrude on harbour seal territory

    NASA Astrophysics Data System (ADS)

    Svensson, Carl Johan

    2012-07-01

    The Kattegat-Skagerrak region on the Swedish west coast is home to an abundant harbour seal population (Phoca vitulina) and a small scattered grey seal population (Halichoerus grypus). In addition, grey seal from the growing population in the Baltic Sea frequently migrate into the Kattegat-Skagerrak. Harbour seals on the west coast of Sweden show relatively high population growth (approximately 9%) compared to the Baltic grey seal in ice-free habitats (approximately 6%), which, in theory, makes harbour seal the stronger competitor of the two in this region. However, incidents of disease in harbour seals that lower population growth are becoming more frequent. These epidemics are primarily caused by the Phocine Distemper Virus (PDV), and may reduce population size with up to 70%. This study models the average development under potential scenarios of competing harbour- and Baltic grey seal populations using Leslie matrices and the Lotka-Volterra model of inter-specific competition. The model is parameterised with previously published data, and resource overlap is incorporated through density dependent pup survival. Using numerical methods short- and long-term abundances are simulated under weak, moderate and strong competition and for different frequencies of PDV epidemics. Results show that the harbour seals are resilient to competition while exerting a negative effect on grey seal abundance under moderate to strong competition. Hence Baltic grey seal benefit from weaker levels of competition. Under moderate and strong competition grey seal abundance is a direct function of the PDV frequency as this reduces the competitive strength of harbour seals. Theoretically this means that higher frequencies of PDV or other pathogens epidemics could facilitate an expansion of Baltic grey seal into Kattegat-Skagerrak. Independent of interaction strength and frequency of epidemics the projected changes to abundances are slow (50-100 years), and even in exceedingly stable

  10. Zinc-fingers and homeoboxes 1 (ZHX1) binds DNA methyltransferase (DNMT) 3B to enhance DNMT3B-mediated transcriptional repression

    SciTech Connect

    Kim, Sung-Hak; Park, Jinah; Choi, Moon-Chang; Kim, Hwang-Phill; Park, Jung-Hyun; Jung, Yeonjoo; Lee, Ju-Hee; Oh, Do-Youn; Im, Seock-Ah; Bang, Yung-Jue; Kim, Tae-You; E-mail: kimty@snu.ac.kr

    2007-04-06

    DNA methyltransferases (DNMT) 3B is a de novo DNMT that represses transcription independent of DNMT activity. In order to gain a better insight into DNMT3B-mediated transcriptional repression, we performed a yeast two-hybrid analysis using DNMT3B as a bait. Of the various binding candidates, ZHX1, a member of zinc-finger and homeobox protein, was found to interact with DNMT3B in vivo and in vitro. N-terminal PWWP domain of DNMT3B was required for its interaction with homeobox motifs of ZHX1. ZHX1 contains nuclear localization signal at C-terminal homeobox motif, and both ZHX1 and DNMT3B were co-localized in nucleus. Furthermore, we found that ZHX1 enhanced the transcriptional repression mediated by DNMT3B when DNMT3B is directly targeted to DNA. These results showed for First the direct linkage between DNMT and zinc-fingers homeoboxes protein, leading to enhanced gene silencing by DNMT3B.

  11. The SSME seal test program: Leakage tests for helically-grooved seals

    NASA Technical Reports Server (NTRS)

    Childs, D. W.

    1983-01-01

    Helically grooved annular seal configurations were tested in highly turbulent flow to determine if reduced leakage and enhanced stability would result from the pumping action of the seal. It was found that: (1) leakage of a helically grooved seals decreases with running speed; (2) leakage reduction due to increased running speed is greater at lower values of R sub a; (3) an asymptote for leakage reduction is indicated with increasing running speed; (4) leakage is reduced by reducing the ridge (minimum) and average clearances; (5) leakage increases with increasing pitch angles and with increasing groove depth. Plain seals with smooth rotors and stators will leak more than a helically grooved seal. It was also found that plain seals with a rough rotor and a rough stator leak less than a properly designed helically grooved seal. A properly designed helically grooved seal consumes at least twice as much power as a conventional annular seal.

  12. Ampoule sealing apparatus and process

    NASA Technical Reports Server (NTRS)

    Debnam, Jr., William J. (Inventor); Clark, Ivan O. (Inventor)

    1988-01-01

    An apparatus 10 for effecting sealing of a fused quartz ampoule 24 while maintaining a vacuum on the ampoule via system 12 is disclosed. A plug 28 of fused quartz is lowered into the vertically disposed ampoule 24 (while maintaining the vacuum thereon) and heat sealed therein to prevent any vapor escape from, or contamination of, the contained semiconductor growth charge 29 during subsequent semiconductor crystal growth processes. A rotary vacuum feed-through mechanism 16 selectively rotates axle 34 and spool 32 to unwind wire 30 for lowering of plug 28 into the reduced diameter portion 24b of ampoule 24. Ampoule 24 is hermatically connected to vacuum housing 18 by quick release flange 20 wherein O-ring 22 retains ampoule 24.

  13. Core disruptive accident margin seal

    DOEpatents

    Garin, John

    1978-01-01

    An apparatus for sealing the annulus defined between a substantially cylindrical rotatable first riser assembly and plug combination disposed in a substantially cylindrical second riser assembly and plug combination of a nuclear reactor system. The apparatus comprises a flexible metal member having a first side attached to one of the riser components and a second side extending toward the other riser component and an actuating mechanism attached to the flexible metal member while extending to an accessible location. When the actuating mechanism is not activated, the flexible metal member does not contact the other riser component thus allowing the free rotation of the riser assembly and plug combination. When desired, the actuating mechanism causes the second side of the flexible metal member to contact the other riser component thereby sealing the annulus between the components.

  14. Core disruptive accident margin seal

    DOEpatents

    Garin, John; Belsick, James C.

    1978-01-01

    An apparatus for sealing the annulus defined between a substantially cylindrical rotatable first riser assembly and plug combination disposed in a substantially cylindrical second riser assembly and plug combination of a nuclear reactor system. The apparatus comprises a flexible member disposed between the first and second riser components and attached to a metal member which is attached to an actuating mechanism. When the actuating mechanism is not actuated, the flexible member does not contact the riser components thus allowing the free rotation of the riser components. When desired, the actuating mechanism causes the flexible member to contact the first and second riser components in a manner to block the annulus defined between the riser components, thereby sealing the annulus between the riser components.

  15. Hermetic seal for a shaft

    NASA Technical Reports Server (NTRS)

    Lombardi, F. (Inventor)

    1982-01-01

    An hermetic seal for a linear rod having a portion thereof projected axially through a port defined in a wall for a pressure chamber and supported thereby for omni-directional motion is described. The seal is characterized by a resilient, impervious, cylindrical body having a first section concentrically related to the shaft and integrally affixed thereto comprising a linear ordered array of annular flutes. A second section integrally is affixed to the wall of the chamber and concentrically related to the port comprising a second linear ordered array of annular flutes. A third section is interposed between the first and second sections and integrally affixed in coaxial alignment therewith comprising an annular ordered array of linear flutes concentrically related to the shaft, whereby axial, angular, and pivotal motion of the rod is accommodated.

  16. Method for forming hermetic seals

    NASA Technical Reports Server (NTRS)

    Gallagher, Brian D.

    1987-01-01

    The firmly adherent film of bondable metal, such as silver, is applied to the surface of glass or other substrate by decomposing a layer of solution of a thermally decomposable metallo-organic deposition (MOD) compound such as silver neodecanoate in xylene. The MOD compound thermally decomposes into metal and gaseous by-products. Sealing is accomplished by depositing a layer of bonding metal, such as solder or a brazing alloy, on the metal film and then forming an assembly with another high melting point metal surface such as a layer of Kovar. When the assembly is heated above the temperature of the solder, the solder flows, wets the adjacent surfaces and forms a hermetic seal between the metal film and metal surface when the assembly cools.

  17. Evaluation of new plastic compression (Ziegler) type of seals for long life planetary batteries

    NASA Technical Reports Server (NTRS)

    Frank, H. A.

    1973-01-01

    A program was initiated to develop improved types of terminal seals for aerospace Ni-Cd batteries. The approach used has not involved attempts, such as employed elsewhere, to improve the ceramic-to-metal seal that is now extensively employed for this application. Rather the approach has been directed toward the development and evaluation of new types of seals. Of prime interest in this initial investigation has been the Ziegler type of compression seal and in particular the injection molded version developed by the Bell Telephone Laboratories (BTL). A number of these units were designed, fabricated, and evaluated on an accelerated life test under a simulated battery environment. Results have shown that there are no major problems involved in scaling up the BTL small-size (5-amp) seal to a larger size (up to 50-amp) seal suitable for most JPL flight batteries. Five out of five such seals successfully completed over 10 months of continuous thermal cycling without developing any leaks greater than 1.8 x to 10 to the minus 9th power atm-cc-He/s.

  18. Hermetically sealed superconducting magnet motor

    DOEpatents

    DeVault, R.C.; McConnell, B.W.; Phillips, B.A.

    1996-07-02

    A hermetically sealed superconducting magnet motor includes a rotor separated from a stator by either a radial gap, an axial gap, or a combined axial and radial gap. Dual conically shaped stators are used in one embodiment to levitate a disc-shaped rotor made of superconducting material within a conduit for moving cryogenic fluid. As the rotor is caused to rotate when the field stator is energized, the fluid is pumped through the conduit. 6 figs.

  19. Hermetically sealed superconducting magnet motor

    DOEpatents

    DeVault, Robert C.; McConnell, Benjamin W.; Phillips, Benjamin A.

    1996-01-01

    A hermetically sealed superconducting magnet motor includes a rotor separated from a stator by either a radial gap, an axial gap, or a combined axial and radial gap. Dual conically shaped stators are used in one embodiment to levitate a disc-shaped rotor made of superconducting material within a conduit for moving cryogenic fluid. As the rotor is caused to rotate when the field stator is energized, the fluid is pumped through the conduit.

  20. Seal for fluid forming tools

    DOEpatents

    Golovashchenko, Sergey Fedorovich [Beverly Hills, MI; Bonnen, John Joseph Francis [Milford, MI

    2012-03-20

    An electro-hydraulic forming tool for forming a sheet metal blank in a one-sided die has first and second rigid rings that engage opposite sides of a sheet metal blank. The rigid rings are contained within slots on a die portion and a hydraulic force applicator portion of the forming tool. The seals are either resiliently biased by an elastomeric member or inherently resiliently biased into contact with the blank.

  1. Improved Mechanical Seals For Tubes

    NASA Technical Reports Server (NTRS)

    Babel, Henry W.; Anderson, Raymond H.; Fuson, Phillip L.; Chickles, Colin D.; Jones, Cherie A.

    1996-01-01

    Improved tube-to-fitting seals made by application of soft metal pieces to either ends of tubes or to interior of fittings. Metal silver, gold, platinum, tin, or other easily malleable metal selected to be inert with fluid of use. Metal plated, evaporated, or sputter-deposited. Coat of soft metal thin, so no changes required in design or tolerances of standard fittings. Technique suitable for hydraulic or other fluid systems in which leakage from mechanically joined sections of tubes occur.

  2. Overview of LIDS Docking and Berthing System Seals

    NASA Technical Reports Server (NTRS)

    Daniels, Christopher C.; Dunlap, Patrick H., Jr.; deGroh, Henry C., III; Steinetz, Bruce M.; Oswald, Jay J.; Smith, Ian

    2007-01-01

    This viewgraph presentation describes the Low Impact Docking System (LIDS) docking and berthing system seals. The contents include: 1) Description of the Application: Low Impact Docking System (LIDS); 2) LIDS Seal Locations: Vehicle Undocked (Hatch Closed); 3) LIDS Seal Locations: Mechanical Pass Thru; 4) LIDS Seal Locations: Electrical and Pyro Connectors; 5) LIDS Seal Locations: Vehicle Docked (Hatches Open); 6) LIDS Seal Locations: Main Interface Seal; 7) Main Interface Seal Challenges and Specifications; 8) Approach; 9) Seal Concepts Under Development/Evaluation; 10) Elastomer Material Evaluations; 11) Evaluation of Relevant Seal Properties; 12) Medium-Scale (12") Gask-O-Seal Compression Tests; 13) Medium-Scale Compression Results; 14) Adhesion Forces of Elliptical Top Gask-o-seals; 15) Medium-Scale Seals; 16) Medium-Scale Leakage Results: Effect of Configuration; 17) Full Scale LIDS Seal Test Rig Development; 18) Materials International Space Station Experiment (MISSE 6A and 6B); and 19) Schedule.

  3. Sealed reed relay limit switches

    NASA Astrophysics Data System (ADS)

    Ivanov, I. P.; Svintsov, G. P.; Yefimova, M. A.; Lebedev, A. V.; Samsonov, Y. P.

    1986-01-01

    Sealed reed relay limit switches are described which overcome the inherent deficiencies traditionally present in these devices: high material, labor consumption, and difficult operation. Series VSG limit switches developed at the All-Union Scientific Research and Technological Planning Institute for Relay Engineering (Cheboksary) for use in buildings are described. The VSG1 has a single switching contact, the VSG2 a single normally open contact, and the VSG3 a single normally closed contact. The VSG1 employs type MKS-27103 sealed reed relays, while the VSG2 and VSG3 employ KEM-1 reed relays. The magnet system, which consists of magnets and a plate, and the sealed reed relay are contained in a nonmagnetic casing with a slit in it. The mathematical expression defining the geometric dimensions of the magnet system is given. The basic parameteres of all three types of switches are tabulated. The VSG series switches can be used to replace VBK and BVK switches, thus saving electricity, labor, and materials, including silver.

  4. Overexpression of a splice variant of DNA methyltransferase 3b, DNMT3b4, associated with DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesis.

    PubMed

    Saito, Yoshimasa; Kanai, Yae; Sakamoto, Michiie; Saito, Hidetsugu; Ishii, Hiromasa; Hirohashi, Setsuo

    2002-07-23

    DNA hypomethylation on pericentromeric satellite regions is an early and frequent event associated with heterochromatin instability during human hepatocarcinogenesis. A DNA methyltransferase, DNMT3b, is required for methylation on pericentromeric satellite regions during mouse development. To clarify the molecular mechanism underlying DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesis, we examined mutations of the DNMT3b gene and mRNA expression levels of splice variants of DNMT3b in noncancerous liver tissues showing chronic hepatitis and cirrhosis, which are considered to be precancerous conditions, and in hepatocellular carcinomas (HCCs). Mutation of the DNMT3b gene was not found in HCCs. Overexpression of DNMT3b4, a splice variant of DNMT3b lacking conserved methyltransferase motifs IX and X, significantly correlated with DNA hypomethylation on pericentromeric satellite regions in precancerous conditions and HCCs (P = 0.0001). In particular, the ratio of expression of DNMT3b4 to that of DNMT3b3, which is the major splice variant in normal liver tissues and retains conserved methyltransferase motifs I, IV, VI, IX, and X, showed significant correlation with DNA hypomethylation (P = 0.009). Transfection of human epithelial 293 cells with DNMT3b4 cDNA induced DNA demethylation on satellite 2 in pericentromeric heterochromatin DNA. These results suggest that overexpression of DNMT3b4, which may lack DNA methyltransferase activity and compete with DNMT3b3 for targeting to pericentromeric satellite regions, results in DNA hypomethylation on these regions, even in precancerous stages, and plays a critical role in human hepatocarcinogenesis by inducing chromosomal instability.

  5. 21 CFR 866.5260 - Complement C3b inactivator immunological test system.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... immunochemical techniques the complement C3b inactivator (a plasma protein) in serum. Complement is a group of serum proteins that destroy infectious agents. Measurement of complement C3b inactivator aids in...

  6. 21 CFR 866.5260 - Complement C3b inactivator immunological test system.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... immunochemical techniques the complement C3b inactivator (a plasma protein) in serum. Complement is a group of serum proteins that destroy infectious agents. Measurement of complement C3b inactivator aids in...

  7. 21 CFR 866.5260 - Complement C3b inactivator immunological test system.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... immunochemical techniques the complement C3b inactivator (a plasma protein) in serum. Complement is a group of serum proteins that destroy infectious agents. Measurement of complement C3b inactivator aids in...

  8. 21 CFR 866.5260 - Complement C3b inactivator immunological test system.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... immunochemical techniques the complement C3b inactivator (a plasma protein) in serum. Complement is a group of serum proteins that destroy infectious agents. Measurement of complement C3b inactivator aids in...

  9. 21 CFR 866.5260 - Complement C3b inactivator immunological test system.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... immunochemical techniques the complement C3b inactivator (a plasma protein) in serum. Complement is a group of serum proteins that destroy infectious agents. Measurement of complement C3b inactivator aids in...

  10. Detection of KS -band Thermal Emission from WASP-3b

    NASA Astrophysics Data System (ADS)

    Zhao, Ming; Milburn, Jennifer; Barman, Travis; Hinkley, Sasha; Swain, Mark R.; Wright, Jason; Monnier, John D.

    2012-03-01

    We report the detection of thermal emission from the hot Jupiter WASP-3b in the KS band, using a newly developed guiding scheme for the WIRC instrument at the Palomar Hale 200 inch telescope. Our new guiding scheme has improved the telescope guiding precision by a factor of ~5-7, significantly reducing the correlated systematics in the measured light curves. This results in the detection of a secondary eclipse with depth of 0.181% ± 0.020% (9σ)—a significant improvement in WIRC's photometric precision and a demonstration of the capability of Palomar/WIRC to produce high-quality measurements of exoplanetary atmospheres. Our measured eclipse depth cannot be explained by model atmospheres with heat redistribution but favors a pure radiative equilibrium case with no redistribution across the surface of the planet. Our measurement also gives an eclipse phase center of 0.5045 ± 0.0020, corresponding to an ecos ω of 0.0070 ± 0.0032. This result is consistent with a circular orbit, although it also suggests that the planet's orbit might be slightly eccentric. The possible non-zero eccentricity provides insight into the tidal circularization process of the star-planet system, but might also have been caused by a second low-mass planet in the system, as suggested by a previous transit timing variation study. More secondary eclipse observations, especially at multiple wavelengths, are necessary to determine the temperature-pressure profile of the planet's atmosphere and shed light on its orbital eccentricity.

  11. The effects of hydrogen in sealed electrical contacts

    NASA Astrophysics Data System (ADS)

    Schubert, Rudolf; Gray, Eoin W.

    1985-12-01

    Hydrogen has been an integral atmospheric component of sealed electrical contacts for decades because of its effect on reliability. It is well known that hydrogen is a needed component to prevent high contact resistance due to carbon-spot formation. This hydrogen benefit has been attributed to hydrogenation of hydrocarbons (HC), enhanced volatilization of previously deposited carbon, enhanced catalytic activity, and thermal cooling. By a variety of physical and chemical laboratory techniques, this paper shows that the hydrogen is not itself directly active. However, some of the hydrogen is converted to water during the glass sealing process. It is this water which plays the active role in the process of quenching carbon formation from hydrocarbon impurities and also acts as a diluent of HC's adsorbed on the contact surface and in the arcing volume. This limits the carbon accumulation on the contacts and increases lifetime reliability.

  12. Integrated Management Program Radioactive Sealed Sources in Egypt

    SciTech Connect

    Hasan, A.; Cochran, J. R.; El-Adham, K.; El-Sorougy, R.

    2003-02-26

    The radioactive materials in ''public'' locations are typically contained in small, stainless steel capsules known as sealed radiation sources (RS). These capsules seal in the radioactive materials, but not the radiation, because it is the radiation that is needed for a wide variety of applications at hospitals, medical clinics, manufacturing plants, universities, construction sites, and other facilities in the public sector. Radiation sources are readily available, and worldwide there are hundreds of thousands of RS. The IMPRSS Project is a cooperative development between the Egyptian Atomic Energy Authority (EAEA), Egyptian Ministry of Health (MOH), Sandia National Laboratories (SNL), New Mexico Tech University (NMT), and Agriculture Cooperative Development International (ACDI/VOCA). SNL will coordinate the work scope between the participant organizations.

  13. Performance Evaluations of Ceramic Wafer Seals

    NASA Technical Reports Server (NTRS)

    Dunlap, Patrick H., Jr.; DeMange, Jeffrey J.; Steinetz, Bruce M.

    2006-01-01

    Future hypersonic vehicles will require high temperature, dynamic seals in advanced ramjet/scramjet engines and on the vehicle airframe to seal the perimeters of movable panels, flaps, and doors. Seal temperatures in these locations can exceed 2000 F, especially when the seals are in contact with hot ceramic matrix composite sealing surfaces. NASA Glenn Research Center is developing advanced ceramic wafer seals to meet the needs of these applications. High temperature scrub tests performed between silicon nitride wafers and carbon-silicon carbide rub surfaces revealed high friction forces and evidence of material transfer from the rub surfaces to the wafer seals. Stickage between adjacent wafers was also observed after testing. Several design changes to the wafer seals were evaluated as possible solutions to these concerns. Wafers with recessed sides were evaluated as a potential means of reducing friction between adjacent wafers. Alternative wafer materials are also being considered as a means of reducing friction between the seals and their sealing surfaces and because the baseline silicon nitride wafer material (AS800) is no longer commercially available.

  14. Seal Technology for Liquid Oxygen (LOX) Turbopumps

    NASA Technical Reports Server (NTRS)

    Shapiro, Wilbur; Hamm, Robert

    1985-01-01

    Two types of advanced seals for liquid oxygen (LOX) turbopumps were investigated. One was a spiral-groove face seal whose function is to seal high-pressure LOX at the impeller end of the turbopump. The other was a floating-ring, Rayleigh-step, helium buffered seal used to prevent LOX ingress to the turbine side of the unit. For each seal type, two sizes were investigated (50 and 20 mm). A turbine-driven test rig was designed and manufactured, and a test program was completed on the 50 mm floating-ring, Rayleigh-step, helium buffered seal. Significant results were: vaporization in the flow path could cause failure by overheating; therefore, the spiral-groove pumping portion of the seal that provides the fluid film must circulate fluid without disruption if vaporization occurs in the sealing dam. This is successfully accomplished by a pressure-balanced spiral-groove concept that is described. The spiral-groove configuration is affected by turbulence in the fluid film and pressure drops due to fluid inertia at sudden contractions. The net results of these effects are deep grooves, large operating films, and high power loss when compared against seals operating with laminar films. Turbulence and inertia are induced by the high-density and low-viscosity characteristics of LOX. The program clearly pointed out the need to consider system environmental factors such as thermal and centrifugal distortions and rotor vibrations in the seal design.

  15. High temperature dynamic engine seal technology development

    NASA Technical Reports Server (NTRS)

    Steinetz, Bruce M.; Dellacorte, Christopher; Machinchick, Michael; Mutharasan, Rajakkannu; Du, Guang-Wu; Ko, Frank; Sirocky, Paul J.; Miller, Jeffrey H.

    1992-01-01

    Combined cycle ramjet/scramjet engines being designed for advanced hypersonic vehicles, including the National Aerospace Plane (NASP), require innovative high temperature dynamic seals to seal the sliding interfaces of the articulated engine panels. New seals are required that will operate hot (1200 to 2000 F), seal pressures ranging from 0 to 100 psi, remain flexible to accommodate significant sidewall distortions, and resist abrasion over the engine's operational life. This report reviews the recent high temperature durability screening assessments of a new braided rope seal concept, braided of emerging high temperature materials, that shows promise of meeting many of the seal demands of hypersonic engines. The paper presents durability data for: (1) the fundamental seal building blocks, a range of candidate ceramic fiber tows; and for (2) braided rope seal subelements scrubbed under engine simulated sliding, temperature, and preload conditions. Seal material/architecture attributes and limitations are identified through the investigations performed. The paper summarizes the current seal technology development status and presents areas in which future work will be performed.

  16. SERS-active nanoparticle aggregate technology for tags and seals

    SciTech Connect

    Brown, Leif O; Montoya, Velma M; Havrilla, George J; Doorn, Stephen K

    2010-06-03

    In this paper, we describe our efforts to create a modern tagging and sealing technology for international safeguards application. Our passive tagging methods are based on SANAs (SERS-Active Nanoparticle Aggregates; SERS: Surface Enhanced Raman Scattering). These SANAs offer robust spectral barcoding capability in an inexpensive tag/seal, with the possibility of rapid in-field verification that requires no human input. At INMM 2009, we introduced SANAs, and showed approaches to integrating our technology with tags under development at Sandia National Laboratories (SNL). Here, we will focus on recent LANL development work, as well as adding additional dimensionality to the barcoding technique. The field of international safeguards employs a broad array of tags, seals, and tamper-indicating devices to assist with identification, tracking, and verification of components and materials. These devices each have unique strengths suited to specific applications, and span a range of technologies from passive metal cup seals and adhesive seals to active, remotely monitored fiber optic seals. Regardless of the technology employed, essential characteristics center around security, environmental and temporal stability, ease of use, and the ability to provide confidence to all parties. Here, we present a new inexpensive tagging technology that will deliver these attributes, while forming the basis of either a new seal, or as a secure layer added to many existing devices. Our approach uses the Surface Enhanced Raman Scattering (SERS) response from SANAs (SERS-Active Nanoparticle Aggregates, Figure 1) to provide a unique identifier or signature for tagging applications. SANAs are formed from gold or silver nanoparticles in the 40-80 nm size range. A chemical dye is installed on the nanoparticle surface, and the nanoparticles are then aggregated into ensembles of {approx}100 to 500 nm diameter, prior to being coated with silica. The silica shell protects the finished SANA from

  17. Expression pattern and clinical significance of DNA methyltransferase 3B variants in gastric carcinoma.

    PubMed

    Su, Xianwei; Lv, Chengyu; Qiao, Fengchang; Qiu, Xuemei; Huang, Wenbin; Wu, Qingxiang; Zhao, Zhujiang; Fan, Hong

    2010-03-01

    The aim of this study was to detect the expression pattern of DNA methyltransferase 3B (DNMT3B) variants in primary gastric cancer (GC) and to explore the clinical significance of DNMT3B variants in gastric carcinogenesis. Specific polymerase chain reaction (PCR) primer sets were designed to distinguish individual DNMT3B variants according to their splicing patterns. Expression levels of DNMT3B variants were assessed by quantitative real-time RT-PCR in gastric cancer tissue, normal gastric mucosae and GC cell lines. The relationship between the expression patterns of the DNMT3B variants and corresponding clinical information was analyzed by observing the expression levels of different variants in the tumors. These results demonstrate that DNMT3B overexpression is related to late phase invasion (P=0.029) and intestinal type (P=0.012) in GC. DNMT3B3 expression was higher in normal tissue, compared to tumor tissue (P=0.033). In contrast, only 18, 32 and 35% of the patient tumors overexpressed DNMT3B1, DNMT3B4 and DNMT3B5, respectively. While taking into account environmental factors (H. pylori, Epstein-Barr virus infection), H. pylori infection elevated DNMT3B1 and DNMT3B3 variants in tumors, while increasing DNMT3B4 in both tumor and non-cancerous tissues. Our findings indicated that the expression of DNMT3B3 is the major splice variant in normal gastric mucosae and may be affected by H. pylori infection. Elevated DNMT3B variants may influence the progression of gastric cancer and may possibly be a powerful indicator for the disease.

  18. Jerantinine A induces tumor-specific cell death through modulation of splicing factor 3b subunit 1 (SF3B1)

    PubMed Central

    Chung, Felicia Fei-Lei; Tan, Perry Faith Tze Ming; Raja, Vijay Joseph; Tan, Boon-Shing; Lim, Kuan-Hon; Kam, Toh-Seok; Hii, Ling-Wei; Tan, Si Hoey; See, Sze-Jia; Tan, Yuen-Fen; Wong, Li-Zhe; Yam, Wai Keat; Mai, Chun Wai; Bradshaw, Tracey D.; Leong, Chee-Onn

    2017-01-01

    Precursor mRNA (pre-mRNA) splicing is catalyzed by a large ribonucleoprotein complex known as the spliceosome. Numerous studies have indicated that aberrant splicing patterns or mutations in spliceosome components, including the splicing factor 3b subunit 1 (SF3B1), are associated with hallmark cancer phenotypes. This has led to the identification and development of small molecules with spliceosome-modulating activity as potential anticancer agents. Jerantinine A (JA) is a novel indole alkaloid which displays potent anti-proliferative activities against human cancer cell lines by inhibiting tubulin polymerization and inducing G2/M cell cycle arrest. Using a combined pooled-genome wide shRNA library screen and global proteomic profiling, we showed that JA targets the spliceosome by up-regulating SF3B1 and SF3B3 protein in breast cancer cells. Notably, JA induced significant tumor-specific cell death and a significant increase in unspliced pre-mRNAs. In contrast, depletion of endogenous SF3B1 abrogated the apoptotic effects, but not the G2/M cell cycle arrest induced by JA. Further analyses showed that JA stabilizes endogenous SF3B1 protein in breast cancer cells and induced dissociation of the protein from the nucleosome complex. Together, these results demonstrate that JA exerts its antitumor activity by targeting SF3B1 and SF3B3 in addition to its reported targeting of tubulin polymerization. PMID:28198434

  19. 18 CFR 3b.204 - Safeguarding information in manual and computer-based record systems.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... information in manual and computer-based record systems. 3b.204 Section 3b.204 Conservation of Power and Water... Collection of Records § 3b.204 Safeguarding information in manual and computer-based record systems. (a) The administrative and physical controls to protect the information in the manual and computer-based record...

  20. 18 CFR 3b.204 - Safeguarding information in manual and computer-based record systems.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... information in manual and computer-based record systems. 3b.204 Section 3b.204 Conservation of Power and Water... Collection of Records § 3b.204 Safeguarding information in manual and computer-based record systems. (a) The administrative and physical controls to protect the information in the manual and computer-based record...