Science.gov

Sample records for 3d activity cliffs

  1. Northern Terra Meridiani Rocks and Cliffs in 3-D

    NASA Technical Reports Server (NTRS)

    2001-01-01

    Extended Mission operations for the Mars Global Surveyor (MGS) Mars Orbiter Camera (MOC) include opportunities that come up about 10 times a week to turn and point the MGS spacecraft so that MOC can photograph a feature of high scientific interest. Many of these images are targeted to the site of a previous MOC image, so that a stereoscopic (3-D) view can be obtained.

    The stereo view, which requires red (left-eye) and blue (right-eye) 3-D glasses to be seen, covers an area approximately 2.3 km (1.4 mi) wide by 6.2 km (3.9 mi) long. The full-resolution view is seen at nearly 1.5 meters (5 ft) per pixel, a scale at which objects the size of airplanes and school buses might be seen.

    The landscape revealed by the 3-D view is a rugged terrain with steep cliffs and no fresh impact craters. This terrain seems most un-Mars-like compared to the typical cratered and dusty views MOC has provided since it began taking data in September 1997. In fact, one of the MOC science team members remarked, 'If I'd seen this landscape used in a movie about Mars five years ago, I'd have said the director had no clue what Mars is supposed to look like.' An irregular depression with a flat, mottled, light-toned floor dominates the scene. Small dark ridges on the depression floor near the top center of the image are dunes or drifts formed by wind transport of sandy sediment. The sharp buttes, mesas, and steep cliffs are all indicators that this terrain consists of a broad exposure of martian bedrock. North is up and sunlight illuminates each picture from the left/upper left.

  2. Reconstructing 3D coastal cliffs from airborne oblique photographs without ground control points

    NASA Astrophysics Data System (ADS)

    Dewez, T. J. B.

    2014-05-01

    Coastal cliff collapse hazard assessment requires measuring cliff face topography at regular intervals. Terrestrial laser scanner techniques have proven useful so far but are expensive to use either through purchasing the equipment or through survey subcontracting. In addition, terrestrial laser surveys take time which is sometimes incompatible with the time during with the beach is accessible at low-tide. By comparison, structure from motion techniques (SFM) are much less costly to implement, and if airborne, acquisition of several kilometers of coastline can be done in a matter of minutes. In this paper, the potential of GPS-tagged oblique airborne photographs and SFM techniques is examined to reconstruct chalk cliff dense 3D point clouds without Ground Control Points (GCP). The focus is put on comparing the relative 3D point of views reconstructed by Visual SFM with their synchronous Solmeta Geotagger Pro2 GPS locations using robust estimators. With a set of 568 oblique photos, shot from the open door of an airplane with a triplet of synchronized Nikon D7000, GPS and SFM-determined view point coordinates converge to X: ±31.5 m; Y: ±39.7 m; Z: ±13.0 m (LE66). Uncertainty in GPS position affects the model scale, angular attitude of the reference frame (the shoreline ends up tilted by 2°) and absolute positioning. Ground Control Points cannot be avoided to orient such models.

  3. Structural analysis of San Leo (RN, Italy) east and north cliffs using 3D point clouds

    NASA Astrophysics Data System (ADS)

    Spreafico, Margherita Cecilia; Bacenetti, Marco; Borgatti, Lisa; Cignetti, Martina; Giardino, Marco; Perotti, Luigi

    2013-04-01

    The town of San Leo, like many others in the historical region of Montefeltro (Northern Apennines, Italy), was built in medieval period on a calcarenite and sandstone slab, bordered by subvertical and overhanging cliffs up to 100 m high, for defense purposes. The slab and the underlying clayey substratum show widespread landslide phenomena: the first is tectonized and crossed by joints and faults, and it is affected by lateral spreading with associated rock falls, topples and tilting. Moreover, the underlying clayey substratum is involved in plastic movements, like earth flows and slides. The main cause of instability in the area, which brings about these movements, is the high deformability contrast between the plate and the underlying clays. The aim of our research is to set up a numerical model that can well describe the processes and take into account the different factors that influence the evolution of the movements. One of these factors is certainly the structural setting of the slab, characterized by several joints and faults; in order to better identify and detect the main joint sets affecting the study area a structural analysis was performed. Up to date, a series of scans of San Leo cliff taken in 2008 and 2011, with a Riegl Z420i were analyzed. Initially, we chose a test area, located in the east side of the cliff, in which analyses were performed using two different softwares: COLTOP 3D and Polyworks. We repeated the analysis using COLTOP for all the east wall and for a part of the north wall, including an area affected by a rock fall in 2006. In the test area we identified five sets with different dips and dip directions. The analysis of the east and north walls permitted to identify eight sets (seven plus the bedding) of discontinuities. We compared these results with previous ones from surveys taken by others authors in some areas and with some preliminary data from a traditional geological survey of the whole area. With traditional methods only a

  4. Composition and topology of activity cliff clusters formed by bioactive compounds.

    PubMed

    Stumpfe, Dagmar; Dimova, Dilyana; Bajorath, Jürgen

    2014-02-24

    The assessment of activity cliffs has thus far mostly focused on compound pairs, although the majority of activity cliffs are not formed in isolation but in a coordinated manner involving multiple active compounds and cliffs. However, the composition of coordinated activity cliff configurations and their topologies are unknown. Therefore, we have identified all activity cliff configurations formed by currently available bioactive compounds and analyzed them in network representations where activity cliff configurations occur as clusters. The composition, topology, frequency of occurrence, and target distribution of activity cliff clusters have been determined. A limited number of large cliff clusters with unique topologies were identified that were centers of activity cliff formation. These clusters originated from a small number of target sets. However, most clusters were of small to moderate size. Three basic topologies were sufficient to describe recurrent activity cliff cluster motifs/topologies. For example, frequently occurring clusters with star topology determined the scale-free character of the global activity cliff network and represented a characteristic activity cliff configuration. Large clusters with complex topology were often found to contain different combinations of basic topologies. Our study provides a first view of activity cliff configurations formed by currently available bioactive compounds and of the recurrent topologies of activity cliff clusters. Activity cliff clusters of defined topology can be selected, and from compounds forming the clusters, SAR information can be obtained. The SAR information of activity cliff clusters sharing a/one specific activity and topology can be compared. PMID:24437577

  5. Method for the evaluation of structure-activity relationship information associated with coordinated activity cliffs.

    PubMed

    Dimova, Dilyana; Stumpfe, Dagmar; Bajorath, Jürgen

    2014-08-14

    Activity cliffs are generally defined as pairs of active compounds having a large difference in potency. Although this definition of activity cliffs focuses on compound pairs, the vast majority of cliffs are formed in a coordinated manner. This means that multiple highly and weakly potent compounds form series of activity cliffs, which often overlap. In activity cliff networks, coordinated cliffs emerge as disjoint activity cliff clusters. Recently, we have identified all cliff clusters from current bioactive compounds and analyzed their topologies. For structure-activity relationship (SAR) analysis, activity cliff clusters are of high interest, since they contain more SAR information than cliffs that are individually considered. For medicinal chemistry applications, a key question becomes how to best extract SAR information from activity cliff clusters. This represents a challenging problem, given the complexity of many activity cliff configurations. Herein we introduce a generally applicable methodology to organize activity cliff clusters on the basis of structural relationships, prioritize clusters, and systematically extract SAR information from them. PMID:25014781

  6. 3-D Resistivity Tomography for Cliff Stability Study at the D-Day Pointe du Hoc Historic Site in Normandy, France

    NASA Astrophysics Data System (ADS)

    Udphuay, S.; Everett, M. E.; Guenther, T.; Warden, R. R.

    2007-12-01

    The D-Day invasion site at Pointe du Hoc in Normandy, France is one of the most important World War II battlefields. The site remains today a valuable historic cultural resource. However the site is vulnerable to cliff collapses that could endanger the observation post building and U.S. Ranger memorial located just landward of the sea stack, and an anti-aircraft gun emplacement, Col. Rudder's command post, located on the cliff edge about 200 m east of the observation post. A 3-D resistivity tomography incorporating extreme topography is used in this study to provide a detailed site stability assessment with special attention to these two buildings. Multi-electrode resistivity measurements were made across the cliff face and along the top of the cliff around the two at-risk buildings to map major subsurface fracture zones and void spaces that could indicate possible accumulations and pathways of groundwater. The ingress of acidic groundwater through the underlying carbonate formations enlarges pre-existing tectonic fractures via limestone dissolution and weakens the overall structural integrity of the cliff. The achieved 3-D resistivity tomograms provide diagnostic subsurface resistivity distributions. Resistive zones associated with subsurface void spaces have been located. These void spaces constitute a stability geohazard as they become significant drainage routes during and after periods of heavy rainfalls.

  7. Accurate 3D point cloud comparison and volumetric change analysis of Terrestrial Laser Scan data in a hard rock coastal cliff environment

    NASA Astrophysics Data System (ADS)

    Earlie, C. S.; Masselink, G.; Russell, P.; Shail, R.; Kingston, K.

    2013-12-01

    Our understanding of the evolution of hard rock coastlines is limited due to the episodic nature and ';slow' rate at which changes occur. High-resolution surveying techniques, such as Terrestrial Laser Scanning (TLS), have just begun to be adopted as a method of obtaining detailed point cloud data to monitor topographical changes over short periods of time (weeks to months). However, the difficulties involved in comparing consecutive point cloud data sets in a complex three-dimensional plane, such as occlusion due to surface roughness and positioning of data capture point as a result of a consistently changing environment (a beach profile), mean that comparing data sets can lead to errors in the region of 10 - 20 cm. Meshing techniques are often used for point cloud data analysis for simple surfaces, but in surfaces such as rocky cliff faces, this technique has been found to be ineffective. Recession rates of hard rock coastlines in the UK are typically determined using aerial photography or airborne LiDAR data, yet the detail of the important changes occurring to the cliff face and toe are missed using such techniques. In this study we apply an algorithm (M3C2 - Multiscale Model to Model Cloud Comparison), initially developed for analysing fluvial morphological change, that directly compares point to point cloud data using surface normals that are consistent with surface roughness and measure the change that occurs along the normal direction (Lague et al., 2013). The surfaces changes are analysed using a set of user defined scales based on surface roughness and registration error. Once the correct parameters are defined, the volumetric cliff face changes are calculated by integrating the mean distance between the point clouds. The analysis has been undertaken at two hard rock sites identified for their active erosion located on the UK's south west peninsular at Porthleven in south west Cornwall and Godrevy in north Cornwall. Alongside TLS point cloud data, in

  8. 3-D Surface Visualization of pH Titration "Topos": Equivalence Point Cliffs, Dilution Ramps, and Buffer Plateaus

    ERIC Educational Resources Information Center

    Smith, Garon C.; Hossain, Md Mainul; MacCarthy, Patrick

    2014-01-01

    3-D topographic surfaces ("topos") can be generated to visualize how pH behaves during titration and dilution procedures. The surfaces are constructed by plotting computed pH values above a composition grid with volume of base added in one direction and overall system dilution on the other. What emerge are surface features that…

  9. How aggressive are coastal cliff environments? Monitoring micro-environmental conditions on an actively eroding rock cliff.

    NASA Astrophysics Data System (ADS)

    Lim, Michael; Rosser, Nicholas; Petley, David; Norman, Emma; Brain, Matthew; Barlow, John

    2010-05-01

    Despite their widespread occurrence, the behaviour of coastal rock cliffs, and in particular the balance between the marine and subaerial conditions that promote erosion, is poorly understood. This is mainly due to a lack of direct, quantitative data on process and response in this type of environment. This paper investigates how near-cliff environmental processes can be associated with the occurrence of rockfalls, which we argue contribute the majority of material lost from coastal cliffs. A detailed recent history of rockfall volumes, dating back to 2003, has been collected using repeat terrestrial laser scans of a 70 m high cliff section on the North Yorkshire coast, UK. This dataset is complimented with a bespoke environmental monitoring system installed upon the cliff face, which allows the influence of weathering and erosion processes on the magnitude and frequency of rockfalls to be analysed. This system is comprised of three instrument clusters at nodes that correspond to three main lithological units of the cliff, hard wired to a communications unit at the top of the cliff face. Data is collected on air temperature, humidity, irradiance, wind and precipitation. Within the near surface of the rock mass we also measure temperature, rock moisture, surface wetness and strain, to allow the direct physical response of the rock to be quantified. The cliff (local) environment monitoring system demonstrates that the rock undergoes significantly greater variability than can be identified from more generic regional weather and tide datasets, predominantly as a function of the angular geometry of the cliff face, resulting in rapid gradients of change. For example, daily variations in temperature and moisture can be seen to have a significant and direct effect on the strain responses of the rock. We seek to establish this as a long-term dataset, to provide a new quantitative assessment of the links between regional and hinterland weather conditions and those found on

  10. 3-D reconstructions of active stars

    NASA Astrophysics Data System (ADS)

    Korhonen, Heidi

    2015-03-01

    Stars are usually faint point sources and investigating their surfaces and interiors observationally is very demanding. Here I give a review on the state-of-the-art observing techniques and recent results on studying interiors and surface features of active stars.

  11. Projection type transparent 3D display using active screen

    NASA Astrophysics Data System (ADS)

    Kamoshita, Hiroki; Yendo, Tomohiro

    2015-05-01

    Equipment to enjoy a 3D image, such as a movie theater, television and so on have been developed many. So 3D video are widely known as a familiar image of technology now. The display representing the 3D image are there such as eyewear, naked-eye, the HMD-type, etc. They has been used for different applications and location. But have not been widely studied for the transparent 3D display. If transparent large 3D display is realized, it is useful to display 3D image overlaid on real scene in some applications such as road sign, shop window, screen in the conference room etc. As a previous study, to produce a transparent 3D display by using a special transparent screen and number of projectors is proposed. However, for smooth motion parallax, many projectors are required. In this paper, we propose a display that has transparency and large display area by time multiplexing projection image in time-division from one or small number of projectors to active screen. The active screen is composed of a number of vertically-long small rotate mirrors. It is possible to realize the stereoscopic viewing by changing the image of the projector in synchronism with the scanning of the beam.3D vision can be realized by light is scanned. Also, the display has transparency, because it is possible to see through the display when the mirror becomes perpendicular to the viewer. We confirmed the validity of the proposed method by using simulation.

  12. 3D MHD Models of Active Region Loops

    NASA Technical Reports Server (NTRS)

    Ofman, Leon

    2004-01-01

    Present imaging and spectroscopic observations of active region loops allow to determine many physical parameters of the coronal loops, such as the density, temperature, velocity of flows in loops, and the magnetic field. However, due to projection effects many of these parameters remain ambiguous. Three dimensional imaging in EUV by the STEREO spacecraft will help to resolve the projection ambiguities, and the observations could be used to setup 3D MHD models of active region loops to study the dynamics and stability of active regions. Here the results of 3D MHD models of active region loops are presented, and the progress towards more realistic 3D MHD models of active regions. In particular the effects of impulsive events on the excitation of active region loop oscillations, and the generation, propagations and reflection of EIT waves are shown. It is shown how 3D MHD models together with 3D EUV observations can be used as a diagnostic tool for active region loop physical parameters, and to advance the science of the sources of solar coronal activity.

  13. Structural interpretation of activity cliffs revealed by systematic analysis of structure-activity relationships in analog series.

    PubMed

    Sisay, Mihiret T; Peltason, Lisa; Bajorath, Jürgen

    2009-10-01

    Discontinuity in structure-activity relationships (SARs) is caused by so-called activity cliffs and represents one of the major caveats in SAR modeling and lead optimization. At activity cliffs, small structural modifications of compounds lead to substantial differences in potency that are essentially unpredictable using quantitative structure-activity relationship (QSAR) methods. In order to better understand SAR discontinuity at the molecular level of detail, we have analyzed different compound series in combinatorial analog graphs and determined substitution patterns that introduce activity cliffs of varying magnitude. So identified SAR determinants were then analyzed on the basis of complex crystal structures to enable a structural interpretation of SAR discontinuity and underlying activity cliffs. In some instances, SAR discontinuity detected within analog series could be well rationalized on the basis of structural data, whereas in others a structural explanation was not possible. This reflects the intrinsic complexity of small molecule SARs and suggests that the analysis of short-range receptor-ligand interactions seen in X-ray structures is insufficient to comprehensively account for SAR discontinuity. However, in other cases, SAR information extracted from ligands was incomplete but could be deduced taking X-ray data into account. Thus, taken together, these findings illustrate the complementarity of ligand-based SAR analysis and structural information. PMID:19761254

  14. C3d adjuvant effects are mediated through the activation of C3d-specific autoreactive T cells

    PubMed Central

    De Groot, Anne S.; Ross, Ted M.; Levitz, Lauren; Messitt, Timothy J.; Tassone, Ryan; Boyle, Christine M.; Vincelli, Amber J.; Moise, Leonard; Martin, William; Knopf, Paul M.

    2014-01-01

    Complement fragment C3d covalently attached to antigens enhances immune responses, particularly for antigens lacking T cell epitopes. Enhancement has been attributed to receptor cross-linking between complement receptor CR2 (CD21) and polysaccharide antigen to surface IgM on naïve B cells. Paradoxically, C3d has still been shown to increase immune responses in CD21 KO mice, suggesting that an auxiliary activation pathway exists. In prior studies, we demonstrated the CD21-independent C3d adjuvant effect might be due to T cell recognition of C3d T helper epitopes processed and presented by MHC class II on the B cell surface. C3d peptide sequences containing concentrated clusters of putative human C3 T cell epitopes were identified using the epitope-mapping algorithm, EpiMatrix. These peptide sequences were synthesized and shown in vitro to bind multiple HLA-DR alleles with high affinity, and induce IFNγ responses in healthy donor PBMCs. In the present studies, we establish further correlations between HLA binding and HLA-specific lymphocyte reactions with select epitope clusters. Additionally, we show that the T cell phenotype of C3d-specific reactive T cells is CD4+CD45RO+ memory T cells. Finally, mutation of a single T cell epitope residing within the P28 peptide segment of C3d resulted in significantly diminished adjuvant activity in BALB/c mice. Collectively, these studies support the hypothesis that the paradoxical enhancement of immune responses by C3d in the absence of CD21 is due to internalization and processing of C3d into peptides that activate autoreactive CD4+ T helper cells in the context of HLA class II. PMID:25385064

  15. Systematic assessment of scaffold hopping versus activity cliff formation across bioactive compound classes following a molecular hierarchy.

    PubMed

    Stumpfe, Dagmar; Dimova, Dilyana; Bajorath, Jürgen

    2015-07-01

    Scaffold hopping and activity cliff formation define opposite ends of the activity landscape feature spectrum. To rationalize these events at the level of scaffolds, active compounds involved in scaffold hopping were required to contain topologically distinct scaffolds but have only limited differences in potency, whereas compounds involved in activity cliffs were required to share the same scaffold but have large differences in potency. A systematic search was carried out for compounds involved in scaffold hopping and/or activity cliff formation. Results obtained for compound data sets covering more than 300 human targets revealed clear trends. If scaffolds represented multiple but fewer than 10 active compounds, nearly 90% of all scaffolds were exclusively involved in hopping events. With increasing compound coverage, the fraction of scaffolds involved in both scaffold hopping and activity cliff formation significantly increased to more than 50%. However, ∼40% of the scaffolds representing large numbers of active compounds continued to be exclusively involved in scaffold hopping. More than 200 scaffolds with broad target coverage were identified that consistently represented potent compounds and yielded an abundance of scaffold hops in the low-nanomolar range. These and other subsets of scaffolds we characterized are of prime interest for structure-activity relationship (SAR) exploration and compound design. Therefore, the complete scaffold classification generated in the course of our analysis is made freely available. PMID:25982076

  16. Fusion of multisensor passive and active 3D imagery

    NASA Astrophysics Data System (ADS)

    Fay, David A.; Verly, Jacques G.; Braun, Michael I.; Frost, Carl E.; Racamato, Joseph P.; Waxman, Allen M.

    2001-08-01

    We have extended our previous capabilities for fusion of multiple passive imaging sensors to now include 3D imagery obtained from a prototype flash ladar. Real-time fusion of low-light visible + uncooled LWIR + 3D LADAR, and SWIR + LWIR + 3D LADAR is demonstrated. Fused visualization is achieved by opponent-color neural networks for passive image fusion, which is then textured upon segmented object surfaces derived from the 3D data. An interactive viewer, coded in Java3D, is used to examine the 3D fused scene in stereo. Interactive designation, learning, recognition and search for targets, based on fused passive + 3D signatures, is achieved using Fuzzy ARTMAP neural networks with a Java-coded GUI. A client-server web-based architecture enables remote users to interact with fused 3D imagery via a wireless palmtop computer.

  17. 3D Actin Network Centerline Extraction with Multiple Active Contours

    PubMed Central

    Xu, Ting; Vavylonis, Dimitrios; Huang, Xiaolei

    2013-01-01

    Fluorescence microscopy is frequently used to study two and three dimensional network structures formed by cytoskeletal polymer fibers such as actin filaments and actin cables. While these cytoskeletal structures are often dilute enough to allow imaging of individual filaments or bundles of them, quantitative analysis of these images is challenging. To facilitate quantitative, reproducible and objective analysis of the image data, we propose a semi-automated method to extract actin networks and retrieve their topology in 3D. Our method uses multiple Stretching Open Active Contours (SOACs) that are automatically initialized at image intensity ridges and then evolve along the centerlines of filaments in the network. SOACs can merge, stop at junctions, and reconfigure with others to allow smooth crossing at junctions of filaments. The proposed approach is generally applicable to images of curvilinear networks with low SNR. We demonstrate its potential by extracting the centerlines of synthetic meshwork images, actin networks in 2D Total Internal Reflection Fluorescence Microscopy images, and 3D actin cable meshworks of live fission yeast cells imaged by spinning disk confocal microscopy. Quantitative evaluation of the method using synthetic images shows that for images with SNR above 5.0, the average vertex error measured by the distance between our result and ground truth is 1 voxel, and the average Hausdorff distance is below 10 voxels. PMID:24316442

  18. 3D Filament Network Segmentation with Multiple Active Contours

    NASA Astrophysics Data System (ADS)

    Xu, Ting; Vavylonis, Dimitrios; Huang, Xiaolei

    2014-03-01

    Fluorescence microscopy is frequently used to study two and three dimensional network structures formed by cytoskeletal polymer fibers such as actin filaments and microtubules. While these cytoskeletal structures are often dilute enough to allow imaging of individual filaments or bundles of them, quantitative analysis of these images is challenging. To facilitate quantitative, reproducible and objective analysis of the image data, we developed a semi-automated method to extract actin networks and retrieve their topology in 3D. Our method uses multiple Stretching Open Active Contours (SOACs) that are automatically initialized at image intensity ridges and then evolve along the centerlines of filaments in the network. SOACs can merge, stop at junctions, and reconfigure with others to allow smooth crossing at junctions of filaments. The proposed approach is generally applicable to images of curvilinear networks with low SNR. We demonstrate its potential by extracting the centerlines of synthetic meshwork images, actin networks in 2D TIRF Microscopy images, and 3D actin cable meshworks of live fission yeast cells imaged by spinning disk confocal microscopy.

  19. Active Exploration of Large 3D Model Repositories.

    PubMed

    Gao, Lin; Cao, Yan-Pei; Lai, Yu-Kun; Huang, Hao-Zhi; Kobbelt, Leif; Hu, Shi-Min

    2015-12-01

    With broader availability of large-scale 3D model repositories, the need for efficient and effective exploration becomes more and more urgent. Existing model retrieval techniques do not scale well with the size of the database since often a large number of very similar objects are returned for a query, and the possibilities to refine the search are quite limited. We propose an interactive approach where the user feeds an active learning procedure by labeling either entire models or parts of them as "like" or "dislike" such that the system can automatically update an active set of recommended models. To provide an intuitive user interface, candidate models are presented based on their estimated relevance for the current query. From the methodological point of view, our main contribution is to exploit not only the similarity between a query and the database models but also the similarities among the database models themselves. We achieve this by an offline pre-processing stage, where global and local shape descriptors are computed for each model and a sparse distance metric is derived that can be evaluated efficiently even for very large databases. We demonstrate the effectiveness of our method by interactively exploring a repository containing over 100 K models. PMID:26529460

  20. Permanent 3D laser scanning system for an active landslide in Gresten (Austria)

    NASA Astrophysics Data System (ADS)

    Canli, Ekrem; Höfle, Bernhard; Hämmerle, Martin; Benni, Thiebes; Glade, Thomas

    2015-04-01

    Terrestrial laser scanners (TLS) have widely been used for high spatial resolution data acquisition of topographic features and geomorphic analyses. Existing applications encompass different landslides including rockfall, translational or rotational landslides, debris flow, but also coastal cliff erosion, braided river evolution or river bank erosion. The main advantages of TLS are (a) the high spatial sampling density of XYZ-measurements (e.g. 1 point every 2-3 mm at 10 m distance), particularly in comparison with the low data density monitoring techniques such as GNSS or total stations, (b) the millimeter accuracy and precision of the range measurement to centimeter accuracy of the final DEM, and (c) the highly dense area-wide scanning that enables to look through vegetation and to measure bare ground. One of its main constraints is the temporal resolution of acquired data due to labor costs and time requirements for field campaigns. Thus, repetition measurements are generally performed only episodically. However, for an increased scientific understanding of the processes as well as for early warning purposes, we present a novel permanent 3D monitoring setup to increase the temporal resolution of TLS measurements. This accounts for different potential monitoring deliverables such as volumetric calculations, spatio-temporal movement patterns, predictions and even alerting. This system was installed at the active Salcher landslide in Gresten (Austria) that is situated in the transition zone of the Gresten Klippenbelt (Helvetic) and the Flyschzone (Penninic). The characteristic lithofacies are the Gresten Beds of Early Jurassic age that are covered by a sequence of marly and silty beds with intercalated sandy limestones. Permanent data acquisition can be implemented into our workflow with any long-range TLS system offering fully automated capturing. We utilize an Optech ILRIS-3D scanner. The time interval between two scans is currently set to 24 hours, but can be

  1. 3D Printing in Instructional Settings: Identifying a Curricular Hierarchy of Activities

    ERIC Educational Resources Information Center

    Brown, Abbie

    2015-01-01

    A report of a year-long study in which the author engaged in 3D printing activity in order to determine how to facilitate and support skill building, concept attainment, and increased confidence with its use among teachers. Use of 3D printing tools and their applications in instructional settings are discussed. A hierarchy of 3D printing…

  2. Energy flow in passive and active 3D cochlear model

    SciTech Connect

    Wang, Yanli; Steele, Charles; Puria, Sunil

    2015-12-31

    Energy flow in the cochlea is an important characteristic of the cochlear traveling wave, and many investigators, such as von Békésy and Lighthill, have discussed this phenomenon. Particularly after the discovery of the motility of the outer hair cells (OHCs), the nature of the power gain of the cochlea has been a fundamental research question. In the present work, direct three-dimensional (3D) calculations of the power on cross sections of the cochlea and on the basilar membrane are performed based on a box model of the mouse cochlea. The distributions of the fluid pressure and fluid velocity in the scala vestibuli are presented. The power output from the OHCs and the power loss due to fluid viscous damping are calculated along the length of the cochlea. This work provides a basis for theoretical calculations of the power gain of the OHCs from mechanical considerations.

  3. Energy flow in passive and active 3D cochlear model

    NASA Astrophysics Data System (ADS)

    Wang, Yanli; Puria, Sunil; Steele, Charles

    2015-12-01

    Energy flow in the cochlea is an important characteristic of the cochlear traveling wave, and many investigators, such as von Békésy and Lighthill, have discussed this phenomenon. Particularly after the discovery of the motility of the outer hair cells (OHCs), the nature of the power gain of the cochlea has been a fundamental research question. In the present work, direct three-dimensional (3D) calculations of the power on cross sections of the cochlea and on the basilar membrane are performed based on a box model of the mouse cochlea. The distributions of the fluid pressure and fluid velocity in the scala vestibuli are presented. The power output from the OHCs and the power loss due to fluid viscous damping are calculated along the length of the cochlea. This work provides a basis for theoretical calculations of the power gain of the OHCs from mechanical considerations.

  4. Spectral ladar: towards active 3D multispectral imaging

    NASA Astrophysics Data System (ADS)

    Powers, Michael A.; Davis, Christopher C.

    2010-04-01

    In this paper we present our Spectral LADAR concept, an augmented implementation of traditional LADAR. This sensor uses a polychromatic source to obtain range-resolved 3D spectral images which are used to identify objects based on combined spatial and spectral features, resolving positions in three dimensions and up to hundreds of meters in distance. We report on a proof-of-concept Spectral LADAR demonstrator that generates spectral point clouds from static scenes. The demonstrator transmits nanosecond supercontinuum pulses generated in a photonic crystal fiber. Currently we use a rapidly tuned receiver with a high-speed InGaAs APD for 25 spectral bands with the future expectation of implementing a linear APD array spectrograph. Each spectral band is independently range resolved with multiple return pulse recognition. This is a critical feature, enabling simultaneous spectral and spatial unmixing of partially obscured objects when not achievable using image fusion of monochromatic LADAR and passive spectral imagers. This enables higher identification confidence in highly cluttered environments such as forested or urban areas (e.g. vehicles behind camouflage or foliage). These environments present challenges for situational awareness and robotic perception which can benefit from the unique attributes of Spectral LADAR. Results from this demonstrator unit are presented for scenes typical of military operations and characterize the operation of the device. The results are discussed here in the context of autonomous vehicle navigation and target recognition.

  5. 3D active stabilization system with sub-micrometer resolution.

    PubMed

    Kursu, Olli; Tuukkanen, Tuomas; Rahkonen, Timo; Vähäsöyrinki, Mikko

    2012-01-01

    Stable positioning between a measurement probe and its target from sub- to few micrometer scales has become a prerequisite in precision metrology and in cellular level measurements from biological tissues. Here we present a 3D stabilization system based on an optoelectronic displacement sensor and custom piezo-actuators driven by a feedback control loop that constantly aims to zero the relative movement between the sensor and the target. We used simulations and prototyping to characterize the developed system. Our results show that 95% attenuation of movement artifacts is achieved at 1 Hz with stabilization performance declining to ca. 70% attenuation at 10 Hz. Stabilization bandwidth is limited by mechanical resonances within the displacement sensor that occur at relatively low frequencies, and are attributable to the sensor's high force sensitivity. We successfully used brain derived micromotion trajectories as a demonstration of complex movement stabilization. The micromotion was reduced to a level of ∼1 µm with nearly 100 fold attenuation at the lower frequencies that are typically associated with physiological processes. These results, and possible improvements of the system, are discussed with a focus on possible ways to increase the sensor's force sensitivity without compromising overall system bandwidth. PMID:22900045

  6. 3D Active Stabilization System with Sub-Micrometer Resolution

    PubMed Central

    Rahkonen, Timo; Vähäsöyrinki, Mikko

    2012-01-01

    Stable positioning between a measurement probe and its target from sub- to few micrometer scales has become a prerequisite in precision metrology and in cellular level measurements from biological tissues. Here we present a 3D stabilization system based on an optoelectronic displacement sensor and custom piezo-actuators driven by a feedback control loop that constantly aims to zero the relative movement between the sensor and the target. We used simulations and prototyping to characterize the developed system. Our results show that 95 % attenuation of movement artifacts is achieved at 1 Hz with stabilization performance declining to ca. 70 % attenuation at 10 Hz. Stabilization bandwidth is limited by mechanical resonances within the displacement sensor that occur at relatively low frequencies, and are attributable to the sensor's high force sensitivity. We successfully used brain derived micromotion trajectories as a demonstration of complex movement stabilization. The micromotion was reduced to a level of ∼1 µm with nearly 100 fold attenuation at the lower frequencies that are typically associated with physiological processes. These results, and possible improvements of the system, are discussed with a focus on possible ways to increase the sensor's force sensitivity without compromising overall system bandwidth. PMID:22900045

  7. Stars of the Big Dipper: A 3-D Vector Activity

    NASA Astrophysics Data System (ADS)

    Kuo, Vince H.; Beichner, Robert J.

    2006-03-01

    Most teachers of introductory physics will agree that many students have difficulty with vectors, so much so that we frequently spend a week at the beginning of the semester presenting material that students should know from previous mathematics courses. This review is often quite abstract, with little or no connection to familiar contexts, and seldom includes any motivation for students to "see it again." In this paper we present a vector activity that attempts to address both these issues using the stars of the Big Dipper, in the constellation Ursa Major, as a memorable context.

  8. 3D visual discomfort predictor: analysis of horizontal disparity and neural activity statistics.

    PubMed

    Park, Jincheol; Oh, Heeseok; Lee, Sanghoon; Bovik, Alan Conrad

    2015-03-01

    Being able to predict the degree of visual discomfort that is felt when viewing stereoscopic 3D (S3D) images is an important goal toward ameliorating causative factors, such as excessive horizontal disparity, misalignments or mismatches between the left and right views of stereo pairs, or conflicts between different depth cues. Ideally, such a model should account for such factors as capture and viewing geometries, the distribution of disparities, and the responses of visual neurons. When viewing modern 3D displays, visual discomfort is caused primarily by changes in binocular vergence while accommodation in held fixed at the viewing distance to a flat 3D screen. This results in unnatural mismatches between ocular fixations and ocular focus that does not occur in normal direct 3D viewing. This accommodation vergence conflict can cause adverse effects, such as headaches, fatigue, eye strain, and reduced visual ability. Binocular vision is ultimately realized by means of neural mechanisms that subserve the sensorimotor control of eye movements. Realizing that the neuronal responses are directly implicated in both the control and experience of 3D perception, we have developed a model-based neuronal and statistical framework called the 3D visual discomfort predictor (3D-VDP)that automatically predicts the level of visual discomfort that is experienced when viewing S3D images. 3D-VDP extracts two types of features: 1) coarse features derived from the statistics of binocular disparities and 2) fine features derived by estimating the neural activity associated with the processing of horizontal disparities. In particular, we deploy a model of horizontal disparity processing in the extrastriate middle temporal region of occipital lobe. We compare the performance of 3D-VDP with other recent discomfort prediction algorithms with respect to correlation against recorded subjective visual discomfort scores,and show that 3D-VDP is statistically superior to the other methods. PMID

  9. ActiveSeismoPick3D - automatic first arrival determination for large active seismic arrays

    NASA Astrophysics Data System (ADS)

    Paffrath, Marcel; Küperkoch, Ludger; Wehling-Benatelli, Sebastian; Friederich, Wolfgang

    2016-04-01

    We developed a tool for automatic determination of first arrivals in active seismic data based on an approach, that utilises higher order statistics (HOS) and the Akaike information criterion (AIC), commonly used in seismology, but not in active seismics. Automatic picking is highly desirable in active seismics as the number of data provided by large seismic arrays rapidly exceeds of what an analyst can evaluate in a reasonable amount of time. To bring the functionality of automatic phase picking into the context of active data, the software package ActiveSeismoPick3D was developed in Python. It uses a modified algorithm for the determination of first arrivals which searches for the HOS maximum in unfiltered data. Additionally, it offers tools for manual quality control and postprocessing, e.g. various visualisation and repicking functionalities. For flexibility, the tool also includes methods for the preparation of geometry information of large seismic arrays and improved interfaces to the Fast Marching Tomography Package (FMTOMO), which can be used for the prediction of travel times and inversion for subsurface properties. Output files are generated in the VTK format, allowing the 3D visualization of e.g. the inversion results. As a test case, a data set consisting of 9216 traces from 64 shots was gathered, recorded at 144 receivers deployed in a regular 2D array of a size of 100 x 100 m. ActiveSeismoPick3D automatically checks the determined first arrivals by a dynamic signal to noise ratio threshold. From the data a 3D model of the subsurface was generated using the export functionality of the package and FMTOMO.

  10. Thermal Modeling Studies for Active Storage Modules in the Calvert Cliffs ISFSI

    SciTech Connect

    Adkins, Harold E.; Fort, James A.; Suffield, Sarah R.; Cuta, Judith M.; Collins, Brian A.

    2013-06-14

    Temperature measurements obtained for two storage modules in the Calvert Cliffs Nuclear Power Station’s Independent Spent Fuel Storage Installation (ISFSI) as part of the Used Fuel Disposition Campaign of the Department of Energy (DOE) were used to perform validation and sensitivity studies on detailed computational fluid dynamics (CFD) models of the concrete storage modules, including the dry storage canister within the modules. The storage modules in the Calvert Cliffs Nuclear Power Station’s ISFSI are a site-specific version of the standard NUHOMS® HSM. The two modules inspected each contained a 24P DSC loaded with 24 CE 14x14 spent fuel assemblies. The thermal analysis was performed using the STAR-CCM+ package, and the models developed for the specific ISFSI modules yielded temperature predictions in actual storage conditions for the concrete structure, the DSC and its contents, including preliminary estimates of fuel cladding temperatures for the used nuclear fuel. The results of this work demonstrate that existing CFD modeling tools can be used to obtain reasonable and accurate detailed representations of spent fuel storage systems with realistic decay heat loadings when the model omits specific conservatisms and bounding assumptions normally used in design-basis and safety-basis calculations. This paper presents sensitivity studies on modeling detail (for the storage module and the DSC), boundary conditions, and decay heat load, to evaluate the effect of the modeling approach on predicted temperatures and temperature distributions. Because nearly all degradation mechanisms for materials and structures comprising dry storage and transportation systems are dependent on temperature, accurate characterization of local temperatures and temperature gradients that the various components of these systems will experience over the entire storage period has been identified as a primary requirement for evaluation of very long term storage of used nuclear fuel.

  11. Frequency of effective wave activity and the recession of coastal bluffs: Calvert Cliffs, Maryland

    USGS Publications Warehouse

    Wilcock, P.R.; Miller, D.S.; Shea, R.H.; Kerkin, R.T.

    1998-01-01

    The Calvert Cliffs, Chesapeake Bay, Maryland, USA, erode by direct wave undercutting or by freeze/thaw erosion accompanied by wave removal of slope debris. Directly undercut slopes recede more rapidly, with long-term rates exceeding 1.0 m/yr; freeze/thaw slopes recede at rates approaching 0.5 m/yr. The frequency of wave height and water level at the shoreline is estimated for eleven sites based on a 37-year wind record, estimates of storm surge, offshore wave geometry, nearshore wave transformation, and breaking wave type. Locations experiencing the largest slope recession are not uniformly those with the largest cumulative wave energy; the resistance to erosion of the slope toe must also be accounted for. An index of relative wave strength is defined as the ratio of wave pressure T and the cohesive strength S of the slope material. For the Calvert Cliffs, a minimum relative wave strength for initiating erosion of intact material is 0.05 < T/S < 0.1. A cumulative duration of ???50 hours per year for T/S ??? 0.1 distinguishes undercut and nonundercut slopes and recession rates greater or lesser than 0.5 m/yr. The relative wave strength index may be used to identify sites at risk of increased erosion. At one site with a small historical erosion rate, the loss of a protective beach and associated decrease in toe elevation caused a positive shift in the frequency of large T/S. Direct wave undercutting and increased slope recession may be anticipated at this site, as indicated by the development of an undercut notch during the course of the study.

  12. Active and interactive floating image display using holographic 3D images

    NASA Astrophysics Data System (ADS)

    Morii, Tsutomu; Sakamoto, Kunio

    2006-08-01

    We developed a prototype tabletop holographic display system. This system consists of the object recognition system and the spatial imaging system. In this paper, we describe the recognition system using an RFID tag and the 3D display system using a holographic technology. A 3D display system is useful technology for virtual reality, mixed reality and augmented reality. We have researched spatial imaging and interaction system. We have ever proposed 3D displays using the slit as a parallax barrier, the lenticular screen and the holographic optical elements(HOEs) for displaying active image 1,2,3. The purpose of this paper is to propose the interactive system using these 3D imaging technologies. In this paper, the authors describe the interactive tabletop 3D display system. The observer can view virtual images when the user puts the special object on the display table. The key technologies of this system are the object recognition system and the spatial imaging display.

  13. Trans3D: a free tool for dynamical visualization of EEG activity transmission in the brain.

    PubMed

    Blinowski, Grzegorz; Kamiński, Maciej; Wawer, Dariusz

    2014-08-01

    The problem of functional connectivity in the brain is in the focus of attention nowadays, since it is crucial for understanding information processing in the brain. A large repertoire of measures of connectivity have been devised, some of them being capable of estimating time-varying directed connectivity. Hence, there is a need for a dedicated software tool for visualizing the propagation of electrical activity in the brain. To this aim, the Trans3D application was developed. It is an open access tool based on widely available libraries and supporting both Windows XP/Vista/7(™), Linux and Mac environments. Trans3D can create animations of activity propagation between electrodes/sensors, which can be placed by the user on the scalp/cortex of a 3D model of the head. Various interactive graphic functions for manipulating and visualizing components of the 3D model and input data are available. An application of the Trans3D tool has helped to elucidate the dynamics of the phenomena of information processing in motor and cognitive tasks, which otherwise would have been very difficult to observe. Trans3D is available at: http://www.eeg.pl/. PMID:24967953

  14. Remembering Cliff Swartz

    NASA Astrophysics Data System (ADS)

    Graf, Erlend H.

    2011-01-01

    In recalling my 40-year friendship with Cliff Swartz, there are naturally a great many interesting anecdotes I could relate. But as amusing or poignant as they might be, they would tend to be rather disjointed—you could never tell what Cliff might do or think of next—without some overarching theme. Accordingly, since this is a magazine about teaching physics, I will concentrate on Cliff's teaching style and some of the many devices he used to enhance it.

  15. Anaglyph: Basalt Cliffs, Patagonia, Argentina

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Basalt cliffs along the northwest edge of the Meseta de Somuncura plateau near Sierra Colorada, Argentina show an unusual and striking pattern of erosion. Stereoscopic observation helps to clarify the landform changing processes active here. Many of the cliffs appear to be rock staircases that have the same color as the plateau's basaltic cap rock. Are these the edges of lower layers in the basalt or are they a train of slivers that are breaking off from, then sliding downslope and away from, the cap rock. They appear to be the latter. Close inspection shows that each stair step is too laterally irregular to be a continuous sheet of bedrock like the cap rock. Also, the steps are not flat but instead are little ridges, as one might expect from broken, tilted, and sliding slices of the cap rock. Stream erosion has cut some gullies into the cliffs and vegetation (appears bright in this infrared image) shows that water springs from and flows down some channels, but land sliding is clearly a major agent of erosion here.

    This anaglyph was generated by first draping a Landsat Thematic Mapper image over a topographic map from the Shuttle Radar Topography Mission, then producing the two differing perspectives, one for each eye. When viewed through special glasses, the result is a vertically exaggerated view of the Earth's surface in its full three dimensions. Anaglyph glasses cover the left eye with a red filter and the right eye with a blue filter.

    Landsat satellites have provided visible light and infrared images of the Earth continuously since 1972. SRTM topographic data match the 30-meter (99-foot) spatial resolution of most Landsat images and provide a valuable complement for studying the historic and growing Landsat data archive. The Landsat 7 Thematic Mapper image used here was provided to the SRTM project by the United States Geological Survey, Earth Resources Observation Systems (EROS) Data Center,Sioux Falls, South Dakota.

    Elevation data used in this

  16. a Comparison Between Active and Passive Techniques for Underwater 3d Applications

    NASA Astrophysics Data System (ADS)

    Bianco, G.; Gallo, A.; Bruno, F.; Muzzupappa, M.

    2011-09-01

    In the field of 3D scanning, there is an increasing need for more accurate technologies to acquire 3D models of close range objects. Underwater exploration, for example, is very hard to perform due to the hostile conditions and the bad visibility of the environment. Some application fields, like underwater archaeology, require to recover tridimensional data of objects that cannot be moved from their site or touched in order to avoid possible damages. Photogrammetry is widely used for underwater 3D acquisition, because it requires just one or two digital still or video cameras to acquire a sequence of images taken from different viewpoints. Stereo systems composed by a pair of cameras are often employed on underwater robots (i.e. ROVs, Remotely Operated Vehicles) and used by scuba divers, in order to survey archaeological sites, reconstruct complex 3D structures in aquatic environment, estimate in situ the length of marine organisms, etc. The stereo 3D reconstruction is based on the triangulation of corresponding points on the two views. This requires to find in both images common points and to match them (correspondence problem), determining a plane that contains the 3D point on the object. Another 3D technique, frequently used in air acquisition, solves this point-matching problem by projecting structured lighting patterns to codify the acquired scene. The corresponding points are identified associating a binary code in both images. In this work we have tested and compared two whole-field 3D imaging techniques (active and passive) based on stereo vision, in underwater environment. A 3D system has been designed, composed by a digital projector and two still cameras mounted in waterproof housing, so that it can perform the various acquisitions without changing the configuration of optical devices. The tests were conducted in a water tank in different turbidity conditions, on objects with different surface properties. In order to simulate a typical seafloor, we used

  17. HOSVD-Based 3D Active Appearance Model: Segmentation of Lung Fields in CT Images.

    PubMed

    Wang, Qingzhu; Kang, Wanjun; Hu, Haihui; Wang, Bin

    2016-07-01

    An Active Appearance Model (AAM) is a computer vision model which can be used to effectively segment lung fields in CT images. However, the fitting result is often inadequate when the lungs are affected by high-density pathologies. To overcome this problem, we propose a Higher-order Singular Value Decomposition (HOSVD)-based Three-dimensional (3D) AAM. An evaluation was performed on 310 diseased lungs form the Lung Image Database Consortium Image Collection. Other contemporary AAMs operate directly on patterns represented by vectors, i.e., before applying the AAM to a 3D lung volume,it has to be vectorized first into a vector pattern by some technique like concatenation. However, some implicit structural or local contextual information may be lost in this transformation. According to the nature of the 3D lung volume, HOSVD is introduced to represent and process the lung in tensor space. Our method can not only directly operate on the original 3D tensor patterns, but also efficiently reduce the computer memory usage. The evaluation resulted in an average Dice coefficient of 97.0 % ± 0.59 %, a mean absolute surface distance error of 1.0403 ± 0.5716 mm, a mean border positioning errors of 0.9187 ± 0.5381 pixel, and a Hausdorff Distance of 20.4064 ± 4.3855, respectively. Experimental results showed that our methods delivered significant and better segmentation results, compared with the three other model-based lung segmentation approaches, namely 3D Snake, 3D ASM and 3D AAM. PMID:27277277

  18. Antiproliferative Activity and Cellular Uptake of Evodiamine and Rutaecarpine Based on 3D Tumor Models.

    PubMed

    Guo, Hui; Liu, Dongmei; Gao, Bin; Zhang, Xiaohui; You, Minli; Ren, Hui; Zhang, Hongbo; Santos, Hélder A; Xu, Feng

    2016-01-01

    Evodiamine (EVO) and rutaecarpine (RUT) are promising anti-tumor drug candidates. The evaluation of the anti-proliferative activity and cellular uptake of EVO and RUT in 3D multicellular spheroids of cancer cells would better recapitulate the native situation and thus better reflect an in vivo response to the treatment. Herein, we employed the 3D culture of MCF-7 and SMMC-7721 cells based on hanging drop method and evaluated the anti-proliferative activity and cellular uptake of EVO and RUT in 3D multicellular spheroids, and compared the results with those obtained from 2D monolayers. The drugs' IC50 values were significantly increased from the range of 6.4-44.1 μM in 2D monolayers to 21.8-138.0 μM in 3D multicellular spheroids, which may be due to enhanced mass barrier and reduced drug penetration in 3D models. The fluorescence of EVO and RUT was measured via fluorescence spectroscopy and the cellular uptake of both drugs was characterized in 2D tumor models. The results showed that the cellular uptake concentrations of RUT increased with increasing drug concentrations. However, the EVO concentrations uptaken by the cells showed only a small change with increasing drug concentrations, which may be due to the different solubility of EVO and Rut in solvents. Overall, this study provided a new vision of the anti-tumor activity of EVO and RUT via 3D multicellular spheroids and cellular uptake through the fluorescence of compounds. PMID:27455219

  19. The Role of Active Exploration of 3D Face Stimuli on Recognition Memory of Facial Information

    ERIC Educational Resources Information Center

    Liu, Chang Hong; Ward, James; Markall, Helena

    2007-01-01

    Research on face recognition has mainly relied on methods in which observers are relatively passive viewers of face stimuli. This study investigated whether active exploration of three-dimensional (3D) face stimuli could facilitate recognition memory. A standard recognition task and a sequential matching task were employed in a yoked design.…

  20. Porosity-controlled eggshell membrane as 3D SERS-active substrate.

    PubMed

    Lin, Pei-Ying; Hsieh, Chiung-Wen; Tsai, Pei-Chuan; Hsieh, Shuchen

    2014-06-01

    We report on the fabrication of a surface-enhanced Raman scattering (SERS) platform, comprised of a three-dimensional (3D) porous eggshell membrane (ESM) scaffold decorated with Ag nanoparticles (NPs). Both native and treated ESM were used, where the treated ESM pore size and fiber crossing density was controlled by timed exposure to hydrogen peroxide (H2O2). Ag NPs were synthesized in situ by reduction of silver nitrate with ascorbic acid. Our results demonstrate that H2O2-treated Ag-ESM provides a more densely packed 3D network of active material, which leads to consistently higher SERS enhancement than untreated Ag-ESM substrates. PMID:24590917

  1. Bottom-up topography assembly into 3D porous scaffold to mediate cell activities.

    PubMed

    Cheng, Delin; Hou, Jie; Hao, Lijing; Cao, Xiaodong; Gao, Huichang; Fu, Xiaoling; Wang, Yingjun

    2016-08-01

    Native cells live in a three-dimensional (3D) extracellular matrix (ECM) capable of regulating cell activities through various physical and chemical factors. Designed topographies have been well proven to trigger significant difference in cell behaviours. However, present topographies are almost all constructed on two-dimensional (2D) substrates like discs and films, which are far from features like 3D and porosity required in application like bone repair. Here we bottom-up assembled poly(lactic-co-glycolic acid)/calcium carbonate (PLGA/CC) microspheres with superficial porous topography intactly into a 3D porous scaffold. Because the scaffold was obtained through a mild technique, the bioactivity of released BMP-2 was well retained. Mouse bone marrow mesenchymal stem cells (mMSCs) were cultured on produced scaffolds having different 3D topographies. It turned out that osteogenic differentiation of mMSCs did respond to the 3D topographies, while proliferation didn't. Gene expression of αv and β1 integrins revealed that adhesion was supposed to be the underlying mechanism for osteogenic response. The study provides insight into enhancing function of practical scaffolds by elaborate topography design. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1056-1063, 2016. PMID:26013977

  2. Overview of passive and active vision techniques for hand-held 3D data acquistion

    NASA Astrophysics Data System (ADS)

    Mada, Sreenivasa K.; Smith, Melvyn L.; Smith, Lyndon N.; Midha, Prema S.

    2003-03-01

    The digitization of the 3D shape of real objects is a rapidly expanding discipline, with a wide variety of applications, including shape acquisition, inspection, reverse engineering, gauging and robot navigation. Developments in computer product design techniques, automated production, and the need for close manufacturing tolerances will be facts of life for the foreseeable future. A growing need exists for fast, accurate, portable, non-contact 3D sensors. However, in order for 3D scanning to become more commonplace, new methods are needed for easily, quickly and robustly acquiring accurate full geometric models of complex objects using low cost technology. In this paper, a brief survey is presented of current scanning technologies available for acquiring range data. An overview is provided of current 3D-shape acquisition using both active and passive vision techniques. Each technique is explained in terms of its configuration, principle of operation, and the inherent advantages and limitations. A separate section then focuses on the implications of scannerless scanning for hand held technology, after which the current status of 3D acquisition using handheld technology, together with related issues concerning implementation, is considered more fully. Finally, conclusions for further developments in handheld devices are discussed. This paper may be of particular benefit to new comers in this field.

  3. 3D pulsed laser-triggered high-speed microfluidic fluorescence-activated cell sorter.

    PubMed

    Chen, Yue; Wu, Ting-Hsiang; Kung, Yu-Chun; Teitell, Michael A; Chiou, Pei-Yu

    2013-11-12

    We report a 3D microfluidic pulsed laser-triggered fluorescence-activated cell sorter capable of sorting at a throughput of 23 000 cells per s with 90% purity in high-purity mode and at a throughput of 45 000 cells per s with 45% purity in enrichment mode in one stage and in a single channel. This performance is realized by exciting laser-induced cavitation bubbles in a 3D PDMS microfluidic channel to generate high-speed liquid jets that deflect detected fluorescent cells and particles focused by 3D sheath flows. The ultrafast switching mechanism (20 μs complete on-off cycle), small liquid jet perturbation volume, and three-dimensional sheath flow focusing for accurate timing control of fast (1.5 m s(-1)) passing cells and particles are three critical factors enabling high-purity sorting at high-throughput in this sorter. PMID:23844418

  4. Evidence for active retreat of a coastal cliff between 3.5 and 12 ka in Cassis (South East France)

    NASA Astrophysics Data System (ADS)

    Recorbet, F.; Rochette, P.; Braucher, R.; Bourlès, D.; Benedetti, L.; Hantz, D.; Finkel, R. C.

    2010-02-01

    This study on the Cap Canaille cliff (N 43.19°, E 5.55°, Cassis, SE France) combines cosmic ray exposure (CRE) dating using two cosmogenic nuclides (in situ-produced 10Be and 36Cl) and morphological analyses to gain a better understanding of a major coastal cliff collapse event. Morphological analysis reveals evidence (cliff morphology, presence of big collapsed blocks) of a possible major collapse of Cap Canaille in the past. Aerial pictures and GIS software allow estimation of a potential collapsed volume of at least 7 × 10 6 m 3, of which roughly 6 × 10 6 m 3 fell into the Mediterranean Sea. In situ-produced 10Be and 36Cl concentrations were measured in samples collected on collapsed block surfaces and in situ-produced 10Be was measured along the cliff face to date the last major collapse event. Statistical analysis of the CRE ages calculated from cliff samples shows that these ages cluster around 3.5 and 6.7 ka, suggesting the existence of a two-step past major collapse. The older ages obtained (at 9 and 12 ka) coincides with the approach of present day sea level, implying a control of sea level on the cliff retreat. The CRE ages calculated from collapsed block samples are more scattered (toward younger ages) due to several geomorphological factors. The estimated collapsed volume associated with the last major collapse around 3.5 ka seems sufficient to have triggered a local tsunami in the Cassis Bay, if it fell at once into the sea.

  5. Synthesis, antifeedant activity against Coleoptera and 3D QSAR study of alpha-asarone derivatives.

    PubMed

    Łozowicka, B; Kaczyński, P; Magdziarz, T; Dubis, A T

    2014-01-01

    For the first time, a set of 56 compounds representing structural derivatives of naturally occurring alpha-asarone as an antifeedants against stored product pests Sitophilus granarius L., Trogoderma granarium Ev., and Tribolium confusum Duv., were subjected to the 3D QSAR studies. Three-dimensional quantitative structure-activity relationships (3D-QSAR) for 56 compounds, including 15 newly synthesized, were performed using comparative molecular field analysis s-CoMFA and SOM-CoMSA techniques. QSAR was conducted based on a combination of biological activity (against Coleoptera larvae and beetles) and various geometrical, topological, quantum-mechanical, electronic, and chromatographic descriptors. The CoMSA formalism coupled with IVE (CoMSA-IVE) allowed us to obtain highly predictive models for Trogoderma granarium Ev. larvae. We have found that this novel method indicates a clear molecular basis for activity and lipophilicity. This investigation will facilitate optimization of the design of new potential antifeedants. PMID:24601760

  6. A 3-D active shape model driven by fuzzy inference: application to cardiac CT and MR.

    PubMed

    van Assen, Hans C; Danilouchkine, Mikhail G; Dirksen, Martijn S; Reiber, Johan H C; Lelieveldt, Boudewijn P F

    2008-09-01

    Manual quantitative analysis of cardiac left ventricular function using Multislice CT and MR is arduous because of the large data volume. In this paper, we present a 3-D active shape model (ASM) for semiautomatic segmentation of cardiac CT and MR volumes, without the requirement of retraining the underlying statistical shape model. A fuzzy c-means based fuzzy inference system was incorporated into the model. Thus, relative gray-level differences instead of absolute gray values were used for classification of 3-D regions of interest (ROIs), removing the necessity of training different models for different modalities/acquisition protocols. The 3-D ASM was evaluated using 25 CT and 15 MR datasets. Automatically generated contours were compared to expert contours in 100 locations. For CT, 82.4% of epicardial contours and 74.1% of endocardial contours had a maximum error of 5 mm along 95% of the contour arc length. For MR, those numbers were 93.2% (epicardium) and 91.4% (endocardium). Volume regression analysis revealed good linear correlations between manual and semiautomatic volumes, r(2) >/= 0.98. This study shows that the fuzzy inference 3-D ASM is a robust promising instrument for semiautomatic cardiac left ventricle segmentation. Without retraining its statistical shape component, it is applicable to routinely acquired CT and MR studies. PMID:18779074

  7. New light sources and sensors for active optical 3D inspection

    NASA Astrophysics Data System (ADS)

    Osten, Wolfgang; Jueptner, Werner P. O.

    1999-11-01

    The implementation of active processing strategies in optical 3D-inspection needs the availability of flexible hardware solutions. The system components illumination and sensor/detector are actively involved in the processing chain by a feedback loop that is controlled by the evaluation process. Therefore this article deals with new light sources and sensor which appeared recently on the market and can be applied successfully for the implementation of active processing principles. Some applications where such new components are used to implement an active measurement strategy are presented.

  8. Multi-shape active composites by 3D printing of digital shape memory polymers

    NASA Astrophysics Data System (ADS)

    Wu, Jiangtao; Yuan, Chao; Ding, Zhen; Isakov, Michael; Mao, Yiqi; Wang, Tiejun; Dunn, Martin L.; Qi, H. Jerry

    2016-04-01

    Recent research using 3D printing to create active structures has added an exciting new dimension to 3D printing technology. After being printed, these active, often composite, materials can change their shape over time; this has been termed as 4D printing. In this paper, we demonstrate the design and manufacture of active composites that can take multiple shapes, depending on the environmental temperature. This is achieved by 3D printing layered composite structures with multiple families of shape memory polymer (SMP) fibers – digital SMPs - with different glass transition temperatures (Tg) to control the transformation of the structure. After a simple single-step thermomechanical programming process, the fiber families can be sequentially activated to bend when the temperature is increased. By tuning the volume fraction of the fibers, bending deformation can be controlled. We develop a theoretical model to predict the deformation behavior for better understanding the phenomena and aiding the design. We also design and print several flat 2D structures that can be programmed to fold and open themselves when subjected to heat. With the advantages of an easy fabrication process and the controllable multi-shape memory effect, the printed SMP composites have a great potential in 4D printing applications.

  9. Multi-shape active composites by 3D printing of digital shape memory polymers.

    PubMed

    Wu, Jiangtao; Yuan, Chao; Ding, Zhen; Isakov, Michael; Mao, Yiqi; Wang, Tiejun; Dunn, Martin L; Qi, H Jerry

    2016-01-01

    Recent research using 3D printing to create active structures has added an exciting new dimension to 3D printing technology. After being printed, these active, often composite, materials can change their shape over time; this has been termed as 4D printing. In this paper, we demonstrate the design and manufacture of active composites that can take multiple shapes, depending on the environmental temperature. This is achieved by 3D printing layered composite structures with multiple families of shape memory polymer (SMP) fibers - digital SMPs - with different glass transition temperatures (Tg) to control the transformation of the structure. After a simple single-step thermomechanical programming process, the fiber families can be sequentially activated to bend when the temperature is increased. By tuning the volume fraction of the fibers, bending deformation can be controlled. We develop a theoretical model to predict the deformation behavior for better understanding the phenomena and aiding the design. We also design and print several flat 2D structures that can be programmed to fold and open themselves when subjected to heat. With the advantages of an easy fabrication process and the controllable multi-shape memory effect, the printed SMP composites have a great potential in 4D printing applications. PMID:27071543

  10. Multi-shape active composites by 3D printing of digital shape memory polymers

    PubMed Central

    Wu, Jiangtao; Yuan, Chao; Ding, Zhen; Isakov, Michael; Mao, Yiqi; Wang, Tiejun; Dunn, Martin L.; Qi, H. Jerry

    2016-01-01

    Recent research using 3D printing to create active structures has added an exciting new dimension to 3D printing technology. After being printed, these active, often composite, materials can change their shape over time; this has been termed as 4D printing. In this paper, we demonstrate the design and manufacture of active composites that can take multiple shapes, depending on the environmental temperature. This is achieved by 3D printing layered composite structures with multiple families of shape memory polymer (SMP) fibers – digital SMPs - with different glass transition temperatures (Tg) to control the transformation of the structure. After a simple single-step thermomechanical programming process, the fiber families can be sequentially activated to bend when the temperature is increased. By tuning the volume fraction of the fibers, bending deformation can be controlled. We develop a theoretical model to predict the deformation behavior for better understanding the phenomena and aiding the design. We also design and print several flat 2D structures that can be programmed to fold and open themselves when subjected to heat. With the advantages of an easy fabrication process and the controllable multi-shape memory effect, the printed SMP composites have a great potential in 4D printing applications. PMID:27071543

  11. Carboxy-Methyl-Cellulose (CMC) hydrogel-filled 3-D scaffold: Preliminary study through a 3-D antiproliferative activity of Centella asiatica extract

    NASA Astrophysics Data System (ADS)

    Aizad, Syazwan; Yahaya, Badrul Hisham; Zubairi, Saiful Irwan

    2015-09-01

    This study focuses on the effects of using the water extract from Centella asiatica on the mortality of human lung cancer cells (A549) with the use of novel 3-D scaffolds infused with CMC hydrogel. A biodegradable polymer, poly (hydroxybutyrate-co-hydroxyvalerate) (PHBV) was used in this study as 3-D scaffolds, with some modifications made by introducing the gel structure on its pore, which provides a great biomimetic microenvironment for cells to grow apart from increasing the interaction between the cells and cell-bioactive extracts. The CMC showed a good hydrophilic characteristic with mean contact angle of 24.30 ± 22.03°. To ensure the CMC gel had good attachments with the scaffolds, a surface treatment was made before the CMC gel was infused into the scaffolds. The results showed that these modified scaffolds contained 42.41 ± 0.14% w/w of CMC gel, which indicated that the gel had already filled up the entire pore of 3-D scaffolds. Besides, the infused hydrogel scaffolds took only 24 hours to be saturated when absorbing the water. The viability of cancer cells by MTS assay after being treated with Centella asiatica showed that the scaffolds infused with CMC hydrogel had the cell viability of 46.89 ± 1.20% followed by porous 3-D model with 57.30 ± 1.60% of cell viability, and the 2-D model with 67.10 ± 1.10% of cell viability. The inhibitory activity in cell viability between 2-D and 3-D models did not differ significantly (p>0.05) due to the limitation of time in incubating the extract with the cell in the 3-D model microenvironment. In conclusion, with the application of 3-D scaffolds infused with CMC hydrogel, the extracts of Centella asiatica has been proven to have the ability to kill cancer cells and have a great potential to become one of the alternative methods in treating cancer patients.

  12. Simulated 3D ultrasound LV cardiac images for active shape model training

    NASA Astrophysics Data System (ADS)

    Butakoff, Constantine; Balocco, Simone; Ordas, Sebastian; Frangi, Alejandro F.

    2007-03-01

    In this paper a study of 3D ultrasound cardiac segmentation using Active Shape Models (ASM) is presented. The proposed approach is based on a combination of a point distribution model constructed from a multitude of high resolution MRI scans and the appearance model obtained from simulated 3D ultrasound images. Usually the appearance model is learnt from a set of landmarked images. The significant level of noise, the low resolution of 3D ultrasound images (3D US) and the frequent failure to capture the complete wall of the left ventricle (LV) makes automatic or manual landmarking difficult. One possible solution is to use artificially simulated 3D US images since the generated images will match exactly the shape in question. In this way, by varying simulation parameters and generating corresponding images, it is possible to obtain a training set where the image matches the shape exactly. In this work the simulation of ultrasound images is performed by a convolutional approach. The evaluation of segmentation accuracy is performed on both simulated and in vivo images. The results obtained on 567 simulated images had an average error of 1.9 mm (1.73 +/- 0.05 mm for epicardium and 2 +/- 0.07 mm for endocardium, with 95% confidence) with voxel size being 1.1 × 1.1 × 0.7 mm. The error on 20 in vivo data was 3.5 mm (3.44 +/- 0.4 mm for epicardium and 3.73 +/- 0.4 mm for endocardium). In most images the model was able to approximate the borders of myocardium even when the latter was indistinguishable from the surrounding tissues.

  13. 3D active surfaces for liver segmentation in multisequence MRI images.

    PubMed

    Bereciartua, Arantza; Picon, Artzai; Galdran, Adrian; Iriondo, Pedro

    2016-08-01

    Biopsies for diagnosis can sometimes be replaced by non-invasive techniques such as CT and MRI. Surgeons require accurate and efficient methods that allow proper segmentation of the organs in order to ensure the most reliable intervention planning. Automated liver segmentation is a difficult and open problem where CT has been more widely explored than MRI. MRI liver segmentation represents a challenge due to the presence of characteristic artifacts, such as partial volumes, noise and low contrast. In this paper, we present a novel method for multichannel MRI automatic liver segmentation. The proposed method consists of the minimization of a 3D active surface by means of the dual approach to the variational formulation of the underlying problem. This active surface evolves over a probability map that is based on a new compact descriptor comprising spatial and multisequence information which is further modeled by means of a liver statistical model. This proposed 3D active surface approach naturally integrates volumetric regularization in the statistical model. The advantages of the compact visual descriptor together with the proposed approach result in a fast and accurate 3D segmentation method. The method was tested on 18 healthy liver studies and results were compared to a gold standard made by expert radiologists. Comparisons with other state-of-the-art approaches are provided by means of nine well established quality metrics. The obtained results improve these methodologies, achieving a Dice Similarity Coefficient of 98.59. PMID:27282235

  14. Evaluating the performance of close-range 3D active vision systems for industrial design applications

    NASA Astrophysics Data System (ADS)

    Beraldin, J.-Angelo; Gaiani, Marco

    2004-12-01

    In recent years, active three-dimensional (3D) active vision systems or range cameras for short have come out of research laboratories to find niche markets in application fields as diverse as industrial design, automotive manufacturing, geomatics, space exploration and cultural heritage to name a few. Many publications address different issues link to 3D sensing and processing but currently these technologies pose a number of challenges to many recent users, i.e., "what are they, how good are they and how do they compare?". The need to understand, test and integrate those range cameras with other technologies, e.g. photogrammetry, CAD, etc. is driven by the quest for optimal resolution, accuracy, speed and cost. Before investing, users want to be certain that a given range camera satisfy their operational requirements. The understanding of the basic theory and best practices associated with those cameras are in fact fundamental to fulfilling the requirements listed above in an optimal way. This paper addresses the evaluation of active 3D range cameras as part of a study to better understand and select one or a number of them to fulfill the needs of industrial design applications. In particular, object material and surface features effect, calibration and performance evaluation are discussed. Results are given for six different range cameras for close range applications.

  15. Evaluating the performance of close-range 3D active vision systems for industrial design applications

    NASA Astrophysics Data System (ADS)

    Beraldin, J.-Angelo; Gaiani, Marco

    2005-01-01

    In recent years, active three-dimensional (3D) active vision systems or range cameras for short have come out of research laboratories to find niche markets in application fields as diverse as industrial design, automotive manufacturing, geomatics, space exploration and cultural heritage to name a few. Many publications address different issues link to 3D sensing and processing but currently these technologies pose a number of challenges to many recent users, i.e., "what are they, how good are they and how do they compare?". The need to understand, test and integrate those range cameras with other technologies, e.g. photogrammetry, CAD, etc. is driven by the quest for optimal resolution, accuracy, speed and cost. Before investing, users want to be certain that a given range camera satisfy their operational requirements. The understanding of the basic theory and best practices associated with those cameras are in fact fundamental to fulfilling the requirements listed above in an optimal way. This paper addresses the evaluation of active 3D range cameras as part of a study to better understand and select one or a number of them to fulfill the needs of industrial design applications. In particular, object material and surface features effect, calibration and performance evaluation are discussed. Results are given for six different range cameras for close range applications.

  16. The Sensitivity of Coastal Cliffs to Changes in Sea Level

    NASA Astrophysics Data System (ADS)

    Rosser, N.; Lim, M.; Petley, D.

    2007-12-01

    The impact of waves upon coastal cliffs is a significant control on erosion and subsequent cliff retreat. It is widely anticipated that climatically-driven sea-level rise will result in an increase in the rate of erosion, and thus the retreat, of coastal cliffs. Quantifying the changes in the rate of coastal erosion remains problematic, primarily due to the difficulty of collecting high-precision and high-frequency monitoring data on both cliff change and the variations in environmental conditions at the coast. In the UK, local authorities now have to produce a "Shoreline Management Plan" (SMP), indicating how the coastline will be managed for the future. This requires the estimation of rates of coastal retreat over the next century, making the impact of sea-level change a critical consideration. This study presents the results from a three year monitoring survey of a section of near-vertical coastal cliffs in north-east England. Data have been collected using a high-resolution terrestrial laser scanner to obtain cliff surfaces. Analysis of successive 3D cliff models is used to derive sequential change, from which the precise nature, geometry and rate of retreat can be measured. In parallel, data has been collected on the micro-seismic impact of waves onto the cliff to gain a direct measure of the delivery of energy at any given sea-level, rather than using a function of wave and tide gauge records. The coastline studied has a significant tidal range, in excess of 6 m, in addition to a large seasonal variability in mean tide heights, allowing a range of sea-level conditions to be assessed. For comparison weather, tide and wave monitoring has been undertaken. The results suggest a close link between the magnitude and frequency of wave impact and the loss of material from the cliff face. Marked changes in wave impact are apparent as the tide level fluctuates on an inter-monthly and inter-annual basis. Thresholds are identified which appear to reflect discrete changes

  17. Fusing Passive and Active Sensed Images to Gain Infrared-Textured 3d Models

    NASA Astrophysics Data System (ADS)

    Weinmann, M.; Hoegner, L.; Leitloff, J.; Stilla, U.; Hinz, S.; Jutzi, B.

    2012-07-01

    Obtaining a 3D description of man-made and natural environments is a basic task in Computer Vision, Photogrammetry and Remote Sensing. New active sensors provide the possibility of capturing range information by images with a single measurement. With this new technique, image-based active ranging is possible which allows for capturing dynamic scenes, e.g. with moving pedestrians or moving vehicles. The currently available range imaging devices usually operate within the close-infrared domain to capture range and furthermore active and passive intensity images. Depending on the application, a 3D description with additional spectral information such as thermal-infrared data can be helpful and offers new opportunities for the detection and interpretation of human subjects and interactions. Therefore, thermal-infrared data combined with range information is promising. In this paper, an approach for mapping thermal-infrared data on range data is proposed. First, a camera calibration is carried out for the range imaging system (PMD[vision] CamCube 2.0) and the thermal-infrared system (InfraTec VarioCAM hr). Subsequently, a registration of close-infrared and thermal infrared intensity images derived from different sensor devices is performed. In this context, wavelength independent properties are selected in order to derive point correspondences between the different spectral domains. Finally, the thermal infrared images are enhanced with information derived from data acquired with the range imaging device and the enhanced IR texture is projected onto the respective 3D point cloud data for gaining appropriate infrared-textured 3D models. The feasibility of the proposed methodology is demonstrated for an experimental setup which is well-suited for investigating these proposed possibilities. Hence, the presented work is a first step towards the development of methods for combined thermal-infrared and range representation.

  18. Diaphragm dome surface segmentation in CT data sets: a 3D active appearance model approach

    NASA Astrophysics Data System (ADS)

    Beichel, Reinhard; Gotschuli, Georg; Sorantin, Erich; Leberl, Franz W.; Sonka, Milan

    2002-05-01

    Knowledge about the location of the diaphragm dome surface, which separates the lungs and the heart from the abdominal cavity, is of vital importance for applications like automated segmentation of adjacent organs (e.g., liver) or functional analysis of the respiratory cycle. We present a new 3D Active Appearance Model (AAM) approach to segmentation of the top layer of the diaphragm dome. The 3D AAM consists of three parts: a 2D closed curve (reference curve), an elevation image and texture layers. The first two parts combined represent 3D shape information and the third part image intensity of the diaphragm dome and the surrounding layers. Differences in height between dome voxels and a reference plane are stored in the elevation image. The reference curve is generated by a parallel projection of the diaphragm dome outline in the axial direction. Landmark point placement is only done on the (2D) reference curve, which can be seen as the bounding curve of the elevation image. Matching is based on a gradient-descent optimization process and uses image intensity appearance around the actual dome shape. Results achieved in 60 computer generated phantom data sets show a high degree of accuracy (positioning error -0.07+/-1.29 mm). Validation using real CT data sets yielded a positioning error of -0.16+/-2.95 mm. Additional training and testing on in-vivo CT image data is ongoing.

  19. Active Landslide Erosion of Mars' North Polar Cliffs: Current Rates, Causes, and Implications

    NASA Astrophysics Data System (ADS)

    Russell, P. S.; Byrne, S.; Pathare, A.

    2014-12-01

    While the North Polar Layered Deposits (NPLD) have long been considered Mars' best climate record, only recently have some of the processes governing the evolution of the NPLD to their current form been discovered and even directly observed. MOC revealed outcrops of an interbedded ice and dark-sand "basal unit" (BU) below steep NPLD scarps. Some bright markings on the BU were suggested to be talus deposits of mass-wasted NPLD ice, caused by undercutting via removal of easily erodible BU sand. In previous work with HiRISE images that confirmed the origin of these deposits, we: detailed their meter-scale morphology, constituent features, and variability; identified fractures of BU ice layers and NPLD scarps as additional mass-wasting facilitators; and, based on inter- and inter-annual monitoring, discovered that new deposits appear over time, thereby establishing mass wasting (of both BU and NPLD ice) as a currently active process. Here, we advance from qualitative descriptions of new, active processes to quantification of the rates at which they are occurring. From ~4 years of HiRISE observations, we cataloged the location, volume, source, and timing constraints of >160 landslide events along ~ 20 km of BU-NPLD scarp. Average rates are ~44 events/yr and ~2000 m3/yr, although 7 large events account for ~3/4 of the volume; this is a very geologically dynamic environment. BU events are more frequent and smaller than NPLD events, reflecting the relative thickness of ice source deposits but also perhaps differing proportions of two causal mechanisms: sand-removal undercutting and thermal-stress induced expansion, contraction, and fracturing. BU events prefer summer over spring; NPLD events strongly prefer fall-winter over spring-summer. We compare this observational data with 1) thermal stress models of the ice scarp to assess consistency in timing of activity, and 2) sublimation models of the ice scarp to assess the relative contributions to scarp retreat - our

  20. Segmentation of the common carotid artery with active shape models from 3D ultrasound images

    NASA Astrophysics Data System (ADS)

    Yang, Xin; Jin, Jiaoying; He, Wanji; Yuchi, Ming; Ding, Mingyue

    2012-03-01

    Carotid atherosclerosis is a major cause of stroke, a leading cause of death and disability. In this paper, we develop and evaluate a new segmentation method for outlining both lumen and adventitia (inner and outer walls) of common carotid artery (CCA) from three-dimensional ultrasound (3D US) images for carotid atherosclerosis diagnosis and evaluation. The data set consists of sixty-eight, 17× 2× 2, 3D US volume data acquired from the left and right carotid arteries of seventeen patients (eight treated with 80mg atorvastain and nine with placebo), who had carotid stenosis of 60% or more, at baseline and after three months of treatment. We investigate the use of Active Shape Models (ASMs) to segment CCA inner and outer walls after statin therapy. The proposed method was evaluated with respect to expert manually outlined boundaries as a surrogate for ground truth. For the lumen and adventitia segmentations, respectively, the algorithm yielded Dice Similarity Coefficient (DSC) of 93.6%+/- 2.6%, 91.8%+/- 3.5%, mean absolute distances (MAD) of 0.28+/- 0.17mm and 0.34 +/- 0.19mm, maximum absolute distances (MAXD) of 0.87 +/- 0.37mm and 0.74 +/- 0.49mm. The proposed algorithm took 4.4 +/- 0.6min to segment a single 3D US images, compared to 11.7+/-1.2min for manual segmentation. Therefore, the method would promote the translation of carotid 3D US to clinical care for the fast, safety and economical monitoring of the atherosclerotic disease progression and regression during therapy.

  1. Alpine Cliff Backwearing Rates Derived From Cosmogenic 10-Be in Active Medial Moraines

    NASA Astrophysics Data System (ADS)

    Ward, D. J.; Anderson, R. S.

    2008-12-01

    We use cosmogenic 10Be concentrations in rock samples from an active, ice-cored medial moraine to constrain glacial valley sidewall backwearing rates in the Kichatna Mountains, Alaska Range, Alaska. Kilometer-tall granite walls that tower over active glaciers are some of the most dramatic landscape features of the Alaska Range. The sheer scale of the relief speaks to the relative rates of valley incision by glaciers and rockwall retreat, but these rates are difficult to determine independently of one another. We present a method that uses cosmogenic nuclides to measure rockwall backwearing rates in glaciated settings on timescales of 103 yr, with a straightforward sampling strategy that exploits active medial moraines. Ablation-dominated medial moraines form by exhumation of debris-rich ice in the ablation zone of a glacier. Exhumed debris insulates the underlying ice and reduces its ablation rate relative to bare ice, promoting formation of a ridge-like, ice cored moraine. The rock debris is primarily derived from supraglacial rockfalls, which become incorporated in the ice along the glacier margins in the accumulation area. These lateral bands of debris-rich ice merge to form a medial debris band when glacial tributaries converge. The debris is minimally mixed until it is exhumed on the moraine crest. In the simplest case, such a system serves as a conveyor belt, bringing material from a specific part of the ablation zone valley wall to a specific point on a medial moraine in the ablation zone. We collected 5 grab samples, each consisting of ~30 2-10 cm rock fragments of the same lithology, from a 4.5 km longitudinal transect on the crest of the medial moraine of the Shadows glacier. We sampled the crest to minimize the amount of post-exhumation transport and mixing that may have occurred; each sample probably contains rocks from only one to a few rockfall events. Measured 10Be concentrations range from 1.5x104 to 3x104 at/g-qtz and are higher downvalley. First

  2. Laser micromachining of through via interconnects in active die for 3-D multichip module

    SciTech Connect

    Chu, D.; Miller, W.D.

    1995-09-01

    One method to increase density in integrated circuits (IC) is to stack die to create a 3-D multichip module (MCM). In the past, special post wafer processing was done to bring interconnects out to the edge of the die. The die were sawed, glued, and stacked. Special processing was done to create interconnects on the edge to provide for interconnects to each of the die. These processes require an IC type fabrication facility (fab) and special processing equipment. In contrast, we have developed packaging assembly methods to created vertical through vias in bond pads of active silicon die, isolate these vias, and metal fill these vias without the use of a special IC fab. These die with through vias can then be joined and stacked to create a 3-D MCM. Vertical through vias in active die are created by laser micromachining using a Nd:YAG laser. Besides the fundamental 1064 nm (infra-red) laser wavelength of a Nd:YAG laser, modifications to our Nd:YAG laser allowed us to generate the second harmonic 532 nm (green) laser wavelength and fourth harmonic 266nm (ultra violet) laser wavelength in laser micromachining for these vias. Experiments were conducted to determine the best laser wavelengths to use for laser micromachining of vertical through vias in order to minimize damage to the active die. Via isolation experiments were done in order to determine the best method in isolating the bond pads of the die. Die thinning techniques were developed to allow for die thickness as thin as 50 {mu}m. This would allow for high 3-D density when the die are stacked. A method was developed to metal fill the vias with solder using a wire bonder with solder wire.

  3. Microtubule regulation of corneal fibroblast morphology and mechanical activity in 3-D culture

    PubMed Central

    Kim, Areum; Petroll, W. Matthew

    2007-01-01

    The purpose of this study was to investigate the role of microtubules in regulating corneal fibroblast structure and mechanical behavior using static (3-D) and dynamic (4-D) imaging of both cells and their surrounding matrix. Human corneal fibroblasts transfected to express GFP-zyxin (to label focal adhesions) or GFP-tubulin (to label microtubules) were plated at low density inside 100 μm thick type I collagen matrices. After 24 hours, the effects of nocodazole (to depolymerize microtubules), cytochalasin D (to disrupt f-actin), and/or Y-27632 (to block Rho-kinase) were evaluated using 3-D and 4-D imaging of both cells and ECM. After 24 hours of incubation, cells had well organized microtubules and prominent focal adhesions, and significant cell-induced matrix compaction was observed. Addition of nocodazole induced rapid microtubule disruption which resulted in Rho activation and additional cellular contraction. The matrix was pulled inward by retracting pseudopodial processes, and focal adhesions appeared to mediate this process, when present. Following 24 hour exposure to nocodazole, there was an even greater increase in both the number of stress fibers and the amount of matrix compaction and alignment at the ends of cells. When Rho-kinase was inhibited, disruption of microtubules resulted in retraction of dendritic cell processes, and rapid formation and extension of lamellipodial processes at random locations along the cell body, eventually leading to a convoluted, disorganized cell shape. These data suggest that microtubules modulate both cellular contractility and local collagen matrix reorganization via regulation of Rho/Rho kinase activity. In addition, microtubules appear to play a central role in dynamic regulation of cell spreading mechanics, morphology and polarity in 3-D culture. PMID:17716657

  4. Dynamic Assessment of Fibroblast Mechanical Activity during Rac-induced Cell Spreading in 3-D Culture

    PubMed Central

    Petroll, W. Matthew; Ma, Lisha; Kim, Areum; Ly, Linda; Vishwanath, Mridula

    2009-01-01

    The goal of this study was to determine the morphological and sub-cellular mechanical effects of Rac activation on fibroblasts within 3-D collagen matrices. Corneal fibroblasts were plated at low density inside 100 μm thick fibrillar collagen matrices and cultured for 1 to 2 days in serum-free media. Time-lapse imaging was then performed using Nomarski DIC. After an acclimation period, perfusion was switched to media containing PDGF. In some experiments, Y-27632 or blebbistatin were used to inhibit Rho-kinase (ROCK) or myosin II, respectively. PDGF activated Rac and induced cell spreading, which resulted in an increase in cell length, cell area, and the number of pseudopodial processes. Tractional forces were generated by extending pseudopodia, as indicated by centripetal displacement and realignment of collagen fibrils. Interestingly, the pattern of pseudopodial extension and local collagen fibril realignment was highly dependent upon the initial orientation of fibrils at the leading edge. Following ROCK or myosin II inhibition, significant ECM relaxation was observed, but small displacements of collagen fibrils continued to be detected at the tips of pseudopodia. Taken together, the data suggests that during Rac-induced cell spreading within 3-D matrices, there is a shift in the distribution of forces from the center to the periphery of corneal fibroblasts. ROCK mediates the generation of large myosin II-based tractional forces during cell spreading within 3-D collagen matrices, however residual forces can be generated at the tips of extending pseudopodia that are both ROCK and myosin II-independent. PMID:18452153

  5. Tubular Enhanced Geodesic Active Contours for Continuum Robot Detection using 3D Ultrasound

    PubMed Central

    Ren, Hongliang; Dupont, Pierre E.

    2013-01-01

    Three dimensional ultrasound is a promising imaging modality for minimally invasive robotic surgery. As the robots are typically metallic, they interact strongly with the sound waves in ways that are not modeled by the ultrasound system’s signal processing algorithms. Consequently, they produce substantial imaging artifacts that can make image guidance difficult, even for experienced surgeons. This paper introduces a new approach for detecting curved continuum robots in 3D ultrasound images. The proposed approach combines geodesic active contours with a speed function that is based on enhancing the “tubularity” of the continuum robot. In particular, it takes advantage of the known robot diameter along its length. It also takes advantage of the fact that the robot surface facing the ultrasound probe provides the most accurate image. This method, termed Tubular Enhanced Geodesic Active Contours (TEGAC), is demonstrated through ex vivo intracardiac experiments to offer superior performance compared to conventional active contours. PMID:24231880

  6. Micro supercapacitors based on a 3D structure with symmetric graphene or activated carbon electrodes

    NASA Astrophysics Data System (ADS)

    Li, Siwei; Wang, Xiaohong; Xing, Hexin; Shen, Caiwei

    2013-11-01

    This paper presents three-dimensional (3D) micro supercapacitors with thick interdigital electrodes supported and separated by SU-8. Nanoporous carbon materials including graphene and activated carbon (AC) are used as active materials in self-supporting composites to build the electrodes. The SU-8 separators provide mechanical support for thick electrodes and allow a considerable amount of material to be loaded in a limited footprint area. The prototypes have been accomplished by a simple microelectromechanical systems (MEMS) fabrication process and sealed by polydimethylsiloxane (PDMS) caps with ionic liquid electrolytes injected into the electrode area. Electrochemical tests demonstrate that the graphene-based prototype with 100 µm thick electrodes shows good power performance and provides a considerable specific capacitance of about 60 mF cm-2. Two AC-based prototypes show larger capacitance of 160 mF cm-2 and 311 mF cm-2 with 100 µm and 200 µm thick electrodes respectively, because of higher volume density of the material. The results demonstrate that both thick 3D electrode structure and volume capacitance of the electrode material are key factors for high-performance micro supercapacitors, which can be potentially used in specific applications such as power suppliers and storage components for harvesters.

  7. Scaling laws of coronal loops compared to a 3D MHD model of an active region

    NASA Astrophysics Data System (ADS)

    Bourdin, Ph.-A.; Bingert, S.; Peter, H.

    2016-04-01

    Context. The structure and heating of coronal loops have been investigated for decades. Established scaling laws relate fundamental quantities like the loop apex temperature, pressure, length, and coronal heating. Aims: We test these scaling laws against a large-scale 3D magneto-hydrodynamics (MHD) model of the solar corona, which became feasible with current high-performance computing. Methods: We drove an active region simulation with photospheric observations and find strong similarities to the observed coronal loops in X-rays and extreme-ultraviolet (EUV) wavelength. A 3D reconstruction of stereoscopic observations shows that our model loops have a realistic spatial structure. We compared scaling laws to our model data extracted along an ensemble of field lines. Finally, we fit a new scaling law that represents hot loops and also cooler structures, which was not possible before based only on observations. Results: Our model data gives some support for scaling laws that were established for hot and EUV-emissive coronal loops. For the Rosner-Tucker-Vaiana (RTV) scaling law we find an offset to our model data, which can be explained by 1D considerations of a static loop with a constant heat input and conduction. With a fit to our model data we set up a new scaling law for the coronal heat input along magnetic field lines. Conclusions: RTV-like scaling laws were fitted to hot loops and therefore do not predict well the coronal heat input for cooler structures that are barely observable. The basic differences between 1D and self-consistent 3D modeling account for deviations between earlier scaling laws and ours. We also conclude that a heating mechanism by MHD-turbulent dissipation within a braided flux tube would heat the corona stronger than is consistent with our model corona.

  8. 3D visualization of movements can amplify motor cortex activation during subsequent motor imagery.

    PubMed

    Sollfrank, Teresa; Hart, Daniel; Goodsell, Rachel; Foster, Jonathan; Tan, Tele

    2015-01-01

    A repetitive movement practice by motor imagery (MI) can influence motor cortical excitability in the electroencephalogram (EEG). This study investigated if a realistic visualization in 3D of upper and lower limb movements can amplify motor related potentials during subsequent MI. We hypothesized that a richer sensory visualization might be more effective during instrumental conditioning, resulting in a more pronounced event related desynchronization (ERD) of the upper alpha band (10-12 Hz) over the sensorimotor cortices thereby potentially improving MI based brain-computer interface (BCI) protocols for motor rehabilitation. The results show a strong increase of the characteristic patterns of ERD of the upper alpha band components for left and right limb MI present over the sensorimotor areas in both visualization conditions. Overall, significant differences were observed as a function of visualization modality (VM; 2D vs. 3D). The largest upper alpha band power decrease was obtained during MI after a 3-dimensional visualization. In total in 12 out of 20 tasks the end-user of the 3D visualization group showed an enhanced upper alpha ERD relative to 2D VM group, with statistical significance in nine tasks.With a realistic visualization of the limb movements, we tried to increase motor cortex activation during subsequent MI. The feedback and the feedback environment should be inherently motivating and relevant for the learner and should have an appeal of novelty, real-world relevance or aesthetic value (Ryan and Deci, 2000; Merrill, 2007). Realistic visual feedback, consistent with the participant's MI, might be helpful for accomplishing successful MI and the use of such feedback may assist in making BCI a more natural interface for MI based BCI rehabilitation. PMID:26347642

  9. 3D visualization of movements can amplify motor cortex activation during subsequent motor imagery

    PubMed Central

    Sollfrank, Teresa; Hart, Daniel; Goodsell, Rachel; Foster, Jonathan; Tan, Tele

    2015-01-01

    A repetitive movement practice by motor imagery (MI) can influence motor cortical excitability in the electroencephalogram (EEG). This study investigated if a realistic visualization in 3D of upper and lower limb movements can amplify motor related potentials during subsequent MI. We hypothesized that a richer sensory visualization might be more effective during instrumental conditioning, resulting in a more pronounced event related desynchronization (ERD) of the upper alpha band (10–12 Hz) over the sensorimotor cortices thereby potentially improving MI based brain-computer interface (BCI) protocols for motor rehabilitation. The results show a strong increase of the characteristic patterns of ERD of the upper alpha band components for left and right limb MI present over the sensorimotor areas in both visualization conditions. Overall, significant differences were observed as a function of visualization modality (VM; 2D vs. 3D). The largest upper alpha band power decrease was obtained during MI after a 3-dimensional visualization. In total in 12 out of 20 tasks the end-user of the 3D visualization group showed an enhanced upper alpha ERD relative to 2D VM group, with statistical significance in nine tasks.With a realistic visualization of the limb movements, we tried to increase motor cortex activation during subsequent MI. The feedback and the feedback environment should be inherently motivating and relevant for the learner and should have an appeal of novelty, real-world relevance or aesthetic value (Ryan and Deci, 2000; Merrill, 2007). Realistic visual feedback, consistent with the participant’s MI, might be helpful for accomplishing successful MI and the use of such feedback may assist in making BCI a more natural interface for MI based BCI rehabilitation. PMID:26347642

  10. 3D active edge silicon sensors with different electrode configurations: Radiation hardness and noise performance

    NASA Astrophysics Data System (ADS)

    Da Viá, C.; Bolle, E.; Einsweiler, K.; Garcia-Sciveres, M.; Hasi, J.; Kenney, C.; Linhart, V.; Parker, Sherwood; Pospisil, S.; Rohne, O.; Slavicek, T.; Watts, S.; Wermes, N.

    2009-06-01

    3D detectors, with electrodes penetrating the entire silicon wafer and active edges, were fabricated at the Stanford Nano Fabrication Facility (SNF), California, USA, with different electrode configurations. After irradiation with neutrons up to a fluence of 8.8×10 15 n eq cm -2, they were characterised using an infrared laser tuned to inject ˜2 minimum ionising particles showing signal efficiencies as high as 66% for the configuration with the shortest (56 μm) inter-electrode spacing. Sensors from the same wafer were also bump-bonded to the ATLAS FE-I3 pixel readout chip and their noise characterised. Most probable signal-to-noise ratios were calculated before and after irradiation to be as good as 38:1 after the highest irradiation level with a substrate thickness of 210 μm. These devices are promising candidates for application at the LHC such as the very forward detectors at ATLAS and CMS, the ATLAS B-Layer replacement and the general pixel upgrade. Moreover, 3D sensors could play a role in applications where high speed, high-resolution detectors are required, such as the vertex locators at the proposed Compact Linear Collider (CLIC) at CERN.

  11. Active learning in the lecture theatre using 3D printed objects.

    PubMed

    Smith, David P

    2016-01-01

    The ability to conceptualize 3D shapes is central to understanding biological processes. The concept that the structure of a biological molecule leads to function is a core principle of the biochemical field. Visualisation of biological molecules often involves vocal explanations or the use of two dimensional slides and video presentations. A deeper understanding of these molecules can however be obtained by the handling of objects. 3D printed biological molecules can be used as active learning tools to stimulate engagement in large group lectures. These models can be used to build upon initial core knowledge which can be delivered in either a flipped form or a more didactic manner. Within the teaching session the students are able to learn by handling, rotating and viewing the objects to gain an appreciation, for example, of an enzyme's active site or the difference between the major and minor groove of DNA. Models and other artefacts can be handled in small groups within a lecture theatre and act as a focal point to generate conversation. Through the approach presented here core knowledge is first established and then supplemented with high level problem solving through a "Think-Pair-Share" cooperative learning strategy. The teaching delivery was adjusted based around experiential learning activities by moving the object from mental cognition and into the physical environment. This approach led to students being able to better visualise biological molecules and a positive engagement in the lecture. The use of objects in teaching allows the lecturer to create interactive sessions that both challenge and enable the student. PMID:27366318

  12. Active learning in the lecture theatre using 3D printed objects

    PubMed Central

    Smith, David P.

    2016-01-01

    The ability to conceptualize 3D shapes is central to understanding biological processes. The concept that the structure of a biological molecule leads to function is a core principle of the biochemical field. Visualisation of biological molecules often involves vocal explanations or the use of two dimensional slides and video presentations. A deeper understanding of these molecules can however be obtained by the handling of objects. 3D printed biological molecules can be used as active learning tools to stimulate engagement in large group lectures. These models can be used to build upon initial core knowledge which can be delivered in either a flipped form or a more didactic manner. Within the teaching session the students are able to learn by handling, rotating and viewing the objects to gain an appreciation, for example, of an enzyme’s active site or the difference between the major and minor groove of DNA. Models and other artefacts can be handled in small groups within a lecture theatre and act as a focal point to generate conversation. Through the approach presented here core knowledge is first established and then supplemented with high level problem solving through a "Think-Pair-Share" cooperative learning strategy. The teaching delivery was adjusted based around experiential learning activities by moving the object from mental cognition and into the physical environment. This approach led to students being able to better visualise biological molecules and a positive engagement in the lecture. The use of objects in teaching allows the lecturer to create interactive sessions that both challenge and enable the student. PMID:27366318

  13. 3D prostate boundary segmentation from ultrasound images using 2D active shape models.

    PubMed

    Hodge, Adam C; Ladak, Hanif M

    2006-01-01

    Boundary outlining, or segmentation, of the prostate is an important task in diagnosis and treatment planning for prostate cancer. This paper describes an algorithm for semi-automatic, three-dimensional (3D) segmentation of the prostate boundary from ultrasound images based on two-dimensional (2D) active shape models (ASM) and rotation-based slicing. Evaluation of the algorithm used distance- and volume-based error metrics to compare algorithm generated boundary outlines to gold standard (manually generated) boundary outlines. The mean absolute distance between the algorithm and gold standard boundaries was 1.09+/-0.49 mm, the average percent absolute volume difference was 3.28+/-3.16%, and a 5x speed increase as compared manual planimetry was achieved. PMID:17946106

  14. A 3D interactive multi-object segmentation tool using local robust statistics driven active contours.

    PubMed

    Gao, Yi; Kikinis, Ron; Bouix, Sylvain; Shenton, Martha; Tannenbaum, Allen

    2012-08-01

    Extracting anatomical and functional significant structures renders one of the important tasks for both the theoretical study of the medical image analysis, and the clinical and practical community. In the past, much work has been dedicated only to the algorithmic development. Nevertheless, for clinical end users, a well designed algorithm with an interactive software is necessary for an algorithm to be utilized in their daily work. Furthermore, the software would better be open sourced in order to be used and validated by not only the authors but also the entire community. Therefore, the contribution of the present work is twofolds: first, we propose a new robust statistics based conformal metric and the conformal area driven multiple active contour framework, to simultaneously extract multiple targets from MR and CT medical imagery in 3D. Second, an open source graphically interactive 3D segmentation tool based on the aforementioned contour evolution is implemented and is publicly available for end users on multiple platforms. In using this software for the segmentation task, the process is initiated by the user drawn strokes (seeds) in the target region in the image. Then, the local robust statistics are used to describe the object features, and such features are learned adaptively from the seeds under a non-parametric estimation scheme. Subsequently, several active contours evolve simultaneously with their interactions being motivated by the principles of action and reaction-this not only guarantees mutual exclusiveness among the contours, but also no longer relies upon the assumption that the multiple objects fill the entire image domain, which was tacitly or explicitly assumed in many previous works. In doing so, the contours interact and converge to equilibrium at the desired positions of the desired multiple objects. Furthermore, with the aim of not only validating the algorithm and the software, but also demonstrating how the tool is to be used, we provide

  15. Synthetic 3D modeling of active regions and simulation of their multi-wavelength emission

    NASA Astrophysics Data System (ADS)

    Nita, Gelu M.; Fleishman, Gregory; Kuznetsov, Alexey A.; Loukitcheva, Maria A.; Viall, Nicholeen M.; Klimchuk, James A.; Gary, Dale E.

    2015-04-01

    To facilitate the study of solar active regions, we have created a synthetic modeling framework that combines 3D magnetic structures obtained from magnetic extrapolations with simplified 1D thermal models of the chromosphere, transition region, and corona. To handle, visualize, and use such synthetic data cubes to compute multi-wavelength emission maps and compare them with observations, we have undertaken a major enhancement of our simulation tools, GX_Simulator (ftp://sohoftp.nascom.nasa.gov/solarsoft/packages/gx_simulator/), developed earlier for modeling emission from flaring loops. The greatly enhanced, object-based architecture, which now runs on Windows, Mac, and UNIX platform, offers important new capabilities that include the ability to either import 3D density and temperature distribution models, or to assign to each individual voxel numerically defined coronal or chromospheric temperature and densities, or coronal Differential Emission Measure distributions. Due to these new capabilities, the GX_Simulator can now apply parametric heating models involving average properties of the magnetic field lines crossing a given voxel volume, as well as compute and investigate the spatial and spectral properties of radio (to be compared with VLA or EOVSA data), (sub-)millimeter (ALMA), EUV (AIA/SDO), and X-ray (RHESSI) emission calculated from the model. The application integrates shared-object libraries containing fast free-free, gyrosynchrotron, and gyroresonance emission codes developed in FORTRAN and C++, and soft and hard X-ray and EUV codes developed in IDL. We use this tool to model and analyze an active region and compare the synthetic emission maps obtained in different wavelengths with observations.This work was partially supported by NSF grants AGS-1250374, AGS-1262772, NASA grant NNX14AC87G, the Marie Curie International Research Staff Exchange Scheme "Radiosun" (PEOPLE-2011-IRSES-295272), RFBR grants 14-02-91157, 15-02-01089, 15-02-03717, 15

  16. TOMO3D: 3-D joint refraction and reflection traveltime tomography parallel code for active-source seismic data—synthetic test

    NASA Astrophysics Data System (ADS)

    Meléndez, A.; Korenaga, J.; Sallarès, V.; Miniussi, A.; Ranero, C. R.

    2015-10-01

    We present a new 3-D traveltime tomography code (TOMO3D) for the modelling of active-source seismic data that uses the arrival times of both refracted and reflected seismic phases to derive the velocity distribution and the geometry of reflecting boundaries in the subsurface. This code is based on its popular 2-D version TOMO2D from which it inherited the methods to solve the forward and inverse problems. The traveltime calculations are done using a hybrid ray-tracing technique combining the graph and bending methods. The LSQR algorithm is used to perform the iterative regularized inversion to improve the initial velocity and depth models. In order to cope with an increased computational demand due to the incorporation of the third dimension, the forward problem solver, which takes most of the run time (˜90 per cent in the test presented here), has been parallelized with a combination of multi-processing and message passing interface standards. This parallelization distributes the ray-tracing and traveltime calculations among available computational resources. The code's performance is illustrated with a realistic synthetic example, including a checkerboard anomaly and two reflectors, which simulates the geometry of a subduction zone. The code is designed to invert for a single reflector at a time. A data-driven layer-stripping strategy is proposed for cases involving multiple reflectors, and it is tested for the successive inversion of the two reflectors. Layers are bound by consecutive reflectors, and an initial velocity model for each inversion step incorporates the results from previous steps. This strategy poses simpler inversion problems at each step, allowing the recovery of strong velocity discontinuities that would otherwise be smoothened.

  17. Chemical Structure-Biological Activity Models for Pharmacophores’ 3D-Interactions

    PubMed Central

    Putz, Mihai V.; Duda-Seiman, Corina; Duda-Seiman, Daniel; Putz, Ana-Maria; Alexandrescu, Iulia; Mernea, Maria; Avram, Speranta

    2016-01-01

    Within medicinal chemistry nowadays, the so-called pharmaco-dynamics seeks for qualitative (for understanding) and quantitative (for predicting) mechanisms/models by which given chemical structure or series of congeners actively act on biological sites either by focused interaction/therapy or by diffuse/hazardous influence. To this aim, the present review exposes three of the fertile directions in approaching the biological activity by chemical structural causes: the special computing trace of the algebraic structure-activity relationship (SPECTRAL-SAR) offering the full analytical counterpart for multi-variate computational regression, the minimal topological difference (MTD) as the revived precursor for comparative molecular field analyses (CoMFA) and comparative molecular similarity indices analysis (CoMSIA); all of these methods and algorithms were presented, discussed and exemplified on relevant chemical medicinal systems as proton pump inhibitors belonging to the 4-indolyl,2-guanidinothiazole class of derivatives blocking the acid secretion from parietal cells in the stomach, the 1-[(2-hydroxyethoxy)-methyl]-6-(phenylthio)thymine congeners’ (HEPT ligands) antiviral activity against Human Immunodeficiency Virus of first type (HIV-1) and new pharmacophores in treating severe genetic disorders (like depression and psychosis), respectively, all involving 3D pharmacophore interactions. PMID:27399692

  18. Chemical Structure-Biological Activity Models for Pharmacophores' 3D-Interactions.

    PubMed

    Putz, Mihai V; Duda-Seiman, Corina; Duda-Seiman, Daniel; Putz, Ana-Maria; Alexandrescu, Iulia; Mernea, Maria; Avram, Speranta

    2016-01-01

    Within medicinal chemistry nowadays, the so-called pharmaco-dynamics seeks for qualitative (for understanding) and quantitative (for predicting) mechanisms/models by which given chemical structure or series of congeners actively act on biological sites either by focused interaction/therapy or by diffuse/hazardous influence. To this aim, the present review exposes three of the fertile directions in approaching the biological activity by chemical structural causes: the special computing trace of the algebraic structure-activity relationship (SPECTRAL-SAR) offering the full analytical counterpart for multi-variate computational regression, the minimal topological difference (MTD) as the revived precursor for comparative molecular field analyses (CoMFA) and comparative molecular similarity indices analysis (CoMSIA); all of these methods and algorithms were presented, discussed and exemplified on relevant chemical medicinal systems as proton pump inhibitors belonging to the 4-indolyl,2-guanidinothiazole class of derivatives blocking the acid secretion from parietal cells in the stomach, the 1-[(2-hydroxyethoxy)-methyl]-6-(phenylthio)thymine congeners' (HEPT ligands) antiviral activity against Human Immunodeficiency Virus of first type (HIV-1) and new pharmacophores in treating severe genetic disorders (like depression and psychosis), respectively, all involving 3D pharmacophore interactions. PMID:27399692

  19. First 3D thermal mapping of an active volcano using an advanced photogrammetric method

    NASA Astrophysics Data System (ADS)

    Antoine, Raphael; Baratoux, David; Lacogne, Julien; Lopez, Teodolina; Fauchard, Cyrille; Bretar, Frédéric; Arab-Sedze, Mélanie; Staudacher, Thomas; Jacquemoud, Stéphane; Pierrot-Deseilligny, Marc

    2014-05-01

    to extract 3D informations from thermal images taken from different positions. This paper presents the first 3D thermal map of an active volcano (Piton de la Fournaise, La Réunion Island) directly generated from 70 thermal images (so-called "stereothermogrammetric" DEM). The data were obtained above Dolomieu caldera by helicopter just before sunrise, during a clear weather in 2008. They were obtained before the eruptive events occurring within the Dolomieu caldera. We used a 28 mm focal FLIR Thermacam PM695 lent by the Piton de la Fournaise Observatory. The thermal images were acquired automatically every 30 seconds with the helicopter flying around the caldera at low altitude (less than 100 m height above the caldera). This survey led to the acquisition of images with a ground pixel size in the range of 1-3 m. A particular attention has been brought to the obtaining of a high overlap percentage (80 percents) for the localization of the maximum tie points on the image. Finally, the acquisition of 70 images allowed the generation of a 3D thermal model of the caldera containing more than 500000 points. i.e. 1 point each 2 m², considering a surface of 106 m² for the Dolomieu caldera. This model is then compared with a DEM recently obtained with the LIDAR method after the eruptive events occurring within Dolomieu. The comparison of these independent methods leads to the validation of the stereothermogrammetric method. It allows the quantification of the thickness of the lava flows within the Dolomieu collapse in 2008 and 2009, i.e. approximately 80 meters, as estimated by previous studies from field observations.

  20. Optical flow 3D segmentation and interpretation: a variational method with active curve evolution and level sets.

    PubMed

    Mitiche, Amar; Sekkati, Hicham

    2006-11-01

    This study investigates a variational, active curve evolution method for dense three-dimentional (3D) segmentation and interpretation of optical flow in an image sequence of a scene containing moving rigid objects viewed by a possibly moving camera. This method jointly performs 3D motion segmentation, 3D interpretation (recovery of 3D structure and motion), and optical flow estimation. The objective functional contains two data terms for each segmentation region, one based on the motion-only equation which relates the essential parameters of 3D rigid body motion to optical flow, and the other on the Horn and Schunck optical flow constraint. It also contains two regularization terms for each region, one for optical flow, the other for the region boundary. The necessary conditions for a minimum of the functional result in concurrent 3D-motion segmentation, by active curve evolution via level sets, and linear estimation of each region essential parameters and optical flow. Subsequently, the screw of 3D motion and regularized relative depth are recovered analytically for each region from the estimated essential parameters and optical flow. Examples are provided which verify the method and its implementation. PMID:17063686

  1. Modulation of cortical activity in 2D versus 3D virtual reality environments: an EEG study.

    PubMed

    Slobounov, Semyon M; Ray, William; Johnson, Brian; Slobounov, Elena; Newell, Karl M

    2015-03-01

    There is a growing empirical evidence that virtual reality (VR) is valuable for education, training, entertaining and medical rehabilitation due to its capacity to represent real-life events and situations. However, the neural mechanisms underlying behavioral confounds in VR environments are still poorly understood. In two experiments, we examined the effect of fully immersive 3D stereoscopic presentations and less immersive 2D VR environments on brain functions and behavioral outcomes. In Experiment 1 we examined behavioral and neural underpinnings of spatial navigation tasks using electroencephalography (EEG). In Experiment 2, we examined EEG correlates of postural stability and balance. Our major findings showed that fully immersive 3D VR induced a higher subjective sense of presence along with enhanced success rate of spatial navigation compared to 2D. In Experiment 1 power of frontal midline EEG (FM-theta) was significantly higher during the encoding phase of route presentation in the 3D VR. In Experiment 2, the 3D VR resulted in greater postural instability and modulation of EEG patterns as a function of 3D versus 2D environments. The findings support the inference that the fully immersive 3D enriched-environment requires allocation of more brain and sensory resources for cognitive/motor control during both tasks than 2D presentations. This is further evidence that 3D VR tasks using EEG may be a promising approach for performance enhancement and potential applications in clinical/rehabilitation settings. PMID:25448267

  2. 3D Fault modeling of the active Chittagong-Myanmar fold belt, Bangladesh

    NASA Astrophysics Data System (ADS)

    Peterson, D. E.; Hubbard, J.; Akhter, S. H.; Shamim, N.

    2013-12-01

    The Chittagong-Myanmar fold belt (CMFB), located in eastern Bangladesh, eastern India and western Myanmar, accommodates east-west shortening at the India-Burma plate boundary. Oblique subduction of the Indian Plate beneath the Burma Plate since the Eocene has led to the development of a large accretionary prism complex, creating a series of north-south trending folds. A continuous sediment record from ~55 Ma to the present has been deposited in the Bengal Basin by the Ganges-Brahmaputra-Meghna rivers, providing an opportunity to learn about the history of tectonic deformation and activity in this fold-and-thrust belt. Surface mapping indicates that the fold-and-thrust belt is characterized by extensive N-S-trending anticlines and synclines in a belt ~150-200 km wide. Seismic reflection profiles from the Chittagong and Chittagong Hill Tracts, Bangladesh, indicate that the anticlines mapped at the surface narrow with depth and extend to ~3.0 seconds TWTT (two-way travel time), or ~6.0 km. The folds of Chittagong and Chittagong Hill Tracts are characterized by doubly plunging box-shaped en-echelon anticlines separated by wide synclines. The seismic data suggest that some of these anticlines are cored by thrust fault ramps that extend to a large-scale décollement that dips gently to the east. Other anticlines may be the result of detachment folding from the same décollement. The décollement likely deepens to the east and intersects with the northerly-trending, oblique-slip Kaladan fault. The CMFB region is bounded to the north by the north-dipping Dauki fault and the Shillong Plateau. The tectonic transition from a wide band of E-W shortening in the south to a narrow zone of N-S shortening along the Dauki fault is poorly understood. We integrate surface and subsurface datasets, including topography, geological maps, seismicity, and industry seismic reflection profiles, into a 3D modeling environment and construct initial 3D surfaces of the major faults in this

  3. Zonation of North Alex Mud Volcano Highlighted by 3-D Active and Passive Seismic Data

    NASA Astrophysics Data System (ADS)

    Bialas, J.; Lefeldt, M. R.; Klaeschen, D.; Papenberg, C. A.; Brueckmann, W.

    2010-12-01

    The West Nile Delta forms part of the source of the large turbiditic Nile Deep Sea Fan. Since the late Miocene sediments have formed an up to 10 km thick pile, which includes about 1 - 3 km of Messinian evaporates. The sediment load of the overburden implies strong overpressures and salt-related tectonic deformation. Both are favourable for fluid migration towards the seafloor guided by the fractured margin. The western deltaic system, Rosetta branch, has formed an 80 km wide continental shelf. Here at 700 m water depth the mud volcano North Alex (NA) developed his circular bathymetric feature, which proved to be an active gas and mud-expelling structure. A 3-D high-resolution multichannel seismic survey (IFM-GEOMAR P-Cable system) was completed across the mud volcano. 3-D time migration provided a 3-D data cube with a 6.25 m grid. Vertical seismic sections did reveal a large set of faults located within the main mud volcano as well as surrounding the structure. Internal faults are mainly related to episodic mud expulsion processes and continuous gas and fluid production. Deep cutting external faults surround the structure in a half circle shape. Horizontal amplitude maps (time slices) of indicate recent activity of these faults even up to the seafloor. High gas saturation of the sediments is indicated by inverted reflection events. In the centre the gas front cuts into the seafloor reflection while it dips down with increasing radius. Only with the small grid resolution inward dipping reflections become visible, which form an upward opened concave reflector plane underlying the top gas front. The interpretation assumes an oval lens shaped body (conduit) saturated with gas at the top of the mud volcano. It provides the upper termination of the mud chimney. This separation is further supported by passive seismic observations. Distant earthquakes can stimulate long-period harmonic oscillations in mud volcanoes. Such oscillations are detectable with three

  4. Determining the 3D Structure of the Corona Using Vertical Height Constraints on Observed Active Region Loops

    NASA Astrophysics Data System (ADS)

    Gary, G. Allen; Hu, Qiang; Lee, Jong Kwan; Aschwanden, Markus J.

    2014-10-01

    The corona associated with an active region is structured by high-temperature, magnetically dominated closed and open loops. The projected 2D geometry of these loops is captured in EUV filtergrams. In this study using SDO/AIA 171 Å filtergrams, we expand our previous method to derive the 3D structure of these loops, independent of heliostereoscopy. We employ an automated loop recognition scheme (Occult-2) and fit the extracted loops with 2D cubic Bézier splines. Utilizing SDO/HMI magnetograms, we extrapolate the magnetic field to obtain simple field models within a rectangular cuboid. Using these models, we minimize the misalignment angle with respect to Bézier control points to extend the splines to 3D (Gary, Hu, and Lee 2014). The derived Bézier control points give the 3D structure of the fitted loops. We demonstrate the process by deriving the position of 3D coronal loops in three active regions (AR 11117, AR 11158, and AR 11283). The numerical minimization process converges and produces 3D curves which are consistent with the height of the loop structures when the active region is seen on the limb. From this we conclude that the method can be important in both determining estimates of the 3D magnetic field structure and determining the best magnetic model among competing advanced magnetohydrodynamics or force-free magnetic-field computer simulations.

  5. 3D Modeling Activity for Novel High Power Electron Guns at SLAC

    SciTech Connect

    Krasnykh, Anatoly

    2003-07-29

    The next generation of powerful electronic devices requires new approaches to overcome the known limitations of existing tube technology. Multi-beam and sheet beam approaches are novel concepts for the high power microwave devices. Direct and indirect modeling methods are being developed at SLAC to meet the new requirements in the 3D modeling. The direct method of solving of Poisson's equations for the multi-beam and sheet beam guns is employed in the TOPAZ 3D tool. The combination of TOPAZ 2D and EGUN (in the beginning) with MAFIA 3D and MAGIC 3D (at the end) is used in an indirect method to model the high power electron guns. Both methods complement each other to get reliable representation of the beam trajectories. Several gun ideas are under consideration at the present time. The collected results of these simulations are discussed.

  6. Micro 3D ERT tomography for data assimilation modelling of active root zone

    NASA Astrophysics Data System (ADS)

    Vanella, Daniela; Busato, Laura; Boaga, Jacopo; Cassiani, Giorgio; Binley, Andrew; Putti, Mario; Consoli, Simona

    2016-04-01

    Within the soil-plant-atmosphere system, root activity plays a fundamental role, as it connects different domains and allows a large part of the water and nutrient exchanges necessary for plant sustenance. The understanding of these processes is not only useful from an environmental point of view, making a fundamental contribution to the understanding of the critical zone dynamics, but also plays a pivotal role in precision agriculture, where the optimisation of water resources exploitation is mandatory and often carried out through deficit irrigation techniques. In this work, we present the results of non-invasive monitoring of the active root zone of two orange trees (Citrus sinensis, cv Tarocco Ippolito) located in an orange orchard in eastern Sicily (Italy) and drip irrigated with two different techniques: partial root drying and 100% crop evapotranspiration. The main goal of the monitoring activity is to assess possible differences between the developed root systems and the root water uptake between the two irrigation strategies. The monitoring is conducted using 3D micro-electrical resistivity tomography (ERT) based on an apparatus composed of a number of micro-boreholes (about 1.2 m deep) housing 12 electrodes each, plus a number of surface electrodes. Time-lapse measurements conducted both with long-term periodicity and short-term repetition before and after irrigation clearly highlight the presence and distribution of root water uptake zone both at shallow and larger depth, likely to correspond to zones utilized during the irrigation period (shallow) and during the time when the crop is not irrigated (deep). Subsidiary information is available in terms of precipitation, sap flow measurements and micrometeorological evapotranspiration estimates. This data ensemble lends itself to the assimilation into a variably saturated flow model, where both soil hydraulic parameters and root distribution shall be identified. Preliminary results in this directions show

  7. Micro 3D ERT tomography for data assimilation modelling of active root zone

    NASA Astrophysics Data System (ADS)

    Cassiani, G.; Boaga, J.; Busato, L.; Vanella, D.; Consoli, S.; Binley, A. M.

    2015-12-01

    Within the soil-plant-atmosphere system, root activity plays a fundamental role, as it connects different domains and allows a large part of the water and nutrient exchanges necessary for plant sustenance. The understanding of these processes is not only useful from an environmental point of view, making a fundamental contribution to the understanding of the critical zone dynamics, but also plays a pivotal role in precision agriculture, where the optimisation of water resources exploitation is mandatory and often carried out through deficit irrigation techniques. In this work, we present the results of non-invasive monitoring of the active root zone of two orange trees (Citrus sinensis, cv Tarocco Ippolito) located in an orange orchard in eastern Sicily (Italy) and drip irrigated with two different techniques: partial root drying and 100% crop evapotranspiration. The main goal of the monitoring activity is to assess possible differences between the developed root systems and the root water uptake between the two irrigation strategies. The monitoring is conducted using 3D micro-electrical resistivity tomography (ERT) based on an apparatus composed of a number of micro-boreholes (about 1.2 m deep) housing 12 electrodes each, plus a number of surface electrodes. Time-lapse measurements conducted both with long-term periodicity and short-term repetition before and after irrigation clearly highlight the presence and distribution of root water uptake zone both at shallow and larger depth, likely to correspond to zones utilized during the irrigation period (shallow) and during the time when the crop is not irrigated (deep). Subsidiary information is available in terms of precipitation, sap flow measurements and micrometeorological evapotranspiration estimates. This data ensemble lends itself to the assimilation into a variably saturated flow model, where both soil hydraulic parameters and root distribution shall be identified. Preliminary results in this directions show

  8. 3D Radiative MHD Modeling of Quiet-Sun Magnetic Activity

    NASA Astrophysics Data System (ADS)

    Kitiashvili, Irina

    2016-05-01

    Quiet-Sun regions that cover most of the solar surface represent a background state that plays an extremely important role in the dynamics and energetics of the solar atmosphere. A clear understanding of these regions is required for accurate interpretation of solar activity events such as emergence of magnetic flux, sunspot formation, and eruptive dynamics. Modern high-resolution observations from ground and space telescopes have revealed a complicated dynamics of turbulent magnetoconvection and its effects in the solar atmosphere and corona, showing intense interactions across different temporal and spatial scales. Interpretation of the observed complex phenomena and understanding of their origins is impossible without advanced numerical models. I will present new results of realistic-type 3D radiative MHD simulations of the upper turbulent convective layer and atmosphere of the Sun. The results reveal the mechanism of formation and properties of the Sun’s “magnetic carpet” controlled by subsurface small-scale dynamo processes, and demonstrate interaction between the subsurface layers and the atmosphere via spontaneous small-scale eruptions and wave phenomena. To link the simulations to solar data the spectro-polarimetric radiative transfer code SPINOR is used to convert the simulated data into the Stokes profiles of various spectral lines, including the SDO and Hinode observables. The results provide a detailed physical understanding of the quiet-Sun dynamics, and show potential for future observations with the DKIST and other large solar telescopes.

  9. Quantifying the 3D Odorant Concentration Field Used by Actively Tracking Blue Crabs

    NASA Astrophysics Data System (ADS)

    Webster, D. R.; Dickman, B. D.; Jackson, J. L.; Weissburg, M. J.

    2007-11-01

    Blue crabs and other aquatic organisms locate food and mates by tracking turbulent odorant plumes. The odorant concentration fluctuates unpredictably due to turbulent transport, and many characteristics of the fluctuation pattern have been hypothesized as useful cues for orienting to the odorant source. To make a direct linkage between tracking behavior and the odorant concentration signal, we developed a measurement system based the laser induced fluorescence technique to quantify the instantaneous 3D concentration field surrounding actively tracking blue crabs. The data suggest a correlation between upstream walking speed and the concentration of the odorant signal arriving at the antennule chemosensors, which are located near the mouth region. More specifically, we note an increase in upstream walking speed when high concentration bursts arrive at the antennules location. We also test hypotheses regarding the ability of blue crabs to steer relative to the plume centerline based on the signal contrast between the chemosensors located on their leg appendages. These chemosensors are located much closer to the substrate compared to the antennules and are separated by the width of the blue crab. In this case, it appears that blue crabs use the bilateral signal comparison to track along the edge of the plume.

  10. Evidence for the buried rim of Campi Flegrei caldera from 3-d active seismic imaging

    NASA Astrophysics Data System (ADS)

    Zollo, A.; Judenherc, S.; Auger, E.; D'Auria, L.; Virieux, J.; Capuano, P.; Chiarabba, C.; de Franco, R.; Makris, J.; Michelini, A.; Musacchio, G.

    2003-10-01

    An extended marine, active seismic survey has been performed on September, 2001 in the gulfs of Naples and Pozzuoli by recording about 5000 shots at a network of 62 sea bottom and 72 on shore seismographs. 3-D images of the shallow caldera structure are obtained from the tomographic inversion of about 77000 first P arrival times using the Benz et al. [1996] tomographic technique. The buried rim of the Campi Flegrei caldera is clearly detected at about 800-2000 m depth, as an anular high P-velocity and high density body. It has a diameter of about 8-12 km and a height of 1-2 km. According to stratigraphic and sonic log data from deep boreholes and tomographic P velocities, the rim is likely formed by solidified lavas and/or tuffs with interbedded lava. This study confirms the existence for a depressed limestone basement beneath the caldera at less than 4 km depth, while no evidence are found for shallower magmatic bodies.

  11. Drag and lift reduction of a 3D bluff-body using active vortex generators

    NASA Astrophysics Data System (ADS)

    Aider, Jean-Luc; Beaudoin, Jean-François; Wesfreid, José Eduardo

    2010-05-01

    In this study, a passive flow control experiment on a 3D bluff-body using vortex generators (VGs) is presented. The bluff-body is a modified Ahmed body (Ahmed in J Fluids Eng 105:429-434 1983) with a curved rear part, instead of a slanted one, so that the location of the flow separation is no longer forced by the geometry. The influence of a line of non-conventional trapezoïdal VGs on the aerodynamic forces (drag and lift) induced on the bluff-body is investigated. The high sensitivity to many geometric (angle between the trapezoïdal element and the wall, spanwise spacing between the VGs, longitudinal location on the curved surface) and physical (freestream velocity) parameters is clearly demonstrated. The maximum drag reduction is -12%, while the maximum global lift reduction can reach more than -60%, with a strong dependency on the freestream velocity. For some configurations, the lift on the rear axle of the model can be inverted (-104%). It is also shown that the VGs are still efficient even downstream of the natural separation line. Finally, a dynamic parameter is chosen and a new set-up with motorized vortex generators is proposed. Thanks to this active device. The optimal configurations depending on two parameters are found more easily, and a significant drag and lift reduction (up to -14% drag reduction) can be reached for different freestream velocities. These results are then analyzed through wall pressure and velocity measurements in the near-wake of the bluff-body with and without control. It appears that the largest drag and lift reduction is clearly associated to a strong increase of the size of the recirculation bubble over the rear slant. Investigation of the velocity field in a cross-section downstream the model reveals that, in the same time, the intensity of the longitudinal trailing vortices is strongly reduced, suggesting that the drag reduction is due to the breakdown of the balance between the separation bubble and the longitudinal vortices

  12. The impact of active versus passive use of 3D technology: a study of dental students at Wuhan University, China.

    PubMed

    Qi, Shengcai; Yan, Yanhong; Li, Rong; Hu, Jian

    2013-11-01

    A variety of computer-based 3D applications are becoming regular tools for dental students for self-learning. This study investigated the learning effectiveness of junior dental students in passively versus actively controlling the 3D virtual scenes of implant dentistry. Participants were randomized into three groups and were exposed to three designs of educational materials: traditional 2D webpages (2D); active-controlling 3D webpages (A3); and passive-controlling 3D webpages (P3). After reviewing the webpages, the participants were asked to complete a posttest to assess the relative quality of information acquisition. Their responses were compared and analyzed. The results indicated that the P3 group received the highest score of 26.4±3.1 on the post-test, significantly better than the A3 group, which had the worst performance with a score of 20.3±4.0. The 2D group received a score of 24.2±4.6. There was a significant correlation between the scores on a mental rotations test and the subjects' performance on the posttest (p<0.001). A serious disadvantage of active control was indicated for individuals with low spatial ability. In 3D virtual reality assisted self-learning, passive control produces higher learning effects compared to active control. Too much active control may generate significantly negative impacts on students, especially for individuals with low spatial ability. PMID:24192420

  13. 3D Stress Modelling of a Neotectonically Active Area in Northwestern Norway

    NASA Astrophysics Data System (ADS)

    Gradmann, Sofie; Keiding, Marie; Olesen, Odleiv; Maystrenko, Yuriy

    2016-04-01

    The Nordland area in NW Norway is one of the tectonically most active areas in Fennoscandia. It exhibits patterns of extension, which are in contradiction to the first-order regional stress pattern which reflects compression from ridge-push. The regional stress field stems from the interaction of ridge push and GIA (glacial isostatic adjustment); the local stress field mainly results from gravitational stresses as well as the flexural effects of sediment erosion and re-deposition. Whereas the first three effects are fairly well constrained, the latter is only poorly known and is the focus of this study. A number of data sets are collected within the project: Seismicity is monitored by a 2-year local seismic network and the stress regime at depth is derived from fault plane solutions. Surface deformation is recorded by a dense GPS network and DInSAR satellites. In-situ stresses are measured in a couple of relevant boreholes. We develop 3D finite element numerical models of crustal scale, using existing geometric constraints from previous geophysical studies. Internal body forces (e.g. variations in topography) already yield significant deviatoric stresses, which are often omitted in stress models. We apply the far-field stress fields (GIA, ridge-push, sediment redistribution) as effective force boundary conditions to the sides or base of the model. This way, we can account for all stress sources at once, but can also vary them separately in order to examine their relative contributions to the observed stress and strain rate fields. We develop a best-fit model using the different seismological and geodetic data sets collected and compiled within the project. Effects of lateral density changes and pre-existing weakness zones on stress localization are studied in connection to observed clusters of enhanced seismic activity.

  14. Learning Dictionaries of Sparse Codes of 3D Movements of Body Joints for Real-Time Human Activity Understanding

    PubMed Central

    Qi, Jin; Yang, Zhiyong

    2014-01-01

    Real-time human activity recognition is essential for human-robot interactions for assisted healthy independent living. Most previous work in this area is performed on traditional two-dimensional (2D) videos and both global and local methods have been used. Since 2D videos are sensitive to changes of lighting condition, view angle, and scale, researchers begun to explore applications of 3D information in human activity understanding in recently years. Unfortunately, features that work well on 2D videos usually don't perform well on 3D videos and there is no consensus on what 3D features should be used. Here we propose a model of human activity recognition based on 3D movements of body joints. Our method has three steps, learning dictionaries of sparse codes of 3D movements of joints, sparse coding, and classification. In the first step, space-time volumes of 3D movements of body joints are obtained via dense sampling and independent component analysis is then performed to construct a dictionary of sparse codes for each activity. In the second step, the space-time volumes are projected to the dictionaries and a set of sparse histograms of the projection coefficients are constructed as feature representations of the activities. Finally, the sparse histograms are used as inputs to a support vector machine to recognize human activities. We tested this model on three databases of human activities and found that it outperforms the state-of-the-art algorithms. Thus, this model can be used for real-time human activity recognition in many applications. PMID:25473850

  15. Learning dictionaries of sparse codes of 3D movements of body joints for real-time human activity understanding.

    PubMed

    Qi, Jin; Yang, Zhiyong

    2014-01-01

    Real-time human activity recognition is essential for human-robot interactions for assisted healthy independent living. Most previous work in this area is performed on traditional two-dimensional (2D) videos and both global and local methods have been used. Since 2D videos are sensitive to changes of lighting condition, view angle, and scale, researchers begun to explore applications of 3D information in human activity understanding in recently years. Unfortunately, features that work well on 2D videos usually don't perform well on 3D videos and there is no consensus on what 3D features should be used. Here we propose a model of human activity recognition based on 3D movements of body joints. Our method has three steps, learning dictionaries of sparse codes of 3D movements of joints, sparse coding, and classification. In the first step, space-time volumes of 3D movements of body joints are obtained via dense sampling and independent component analysis is then performed to construct a dictionary of sparse codes for each activity. In the second step, the space-time volumes are projected to the dictionaries and a set of sparse histograms of the projection coefficients are constructed as feature representations of the activities. Finally, the sparse histograms are used as inputs to a support vector machine to recognize human activities. We tested this model on three databases of human activities and found that it outperforms the state-of-the-art algorithms. Thus, this model can be used for real-time human activity recognition in many applications. PMID:25473850

  16. 3D Modelling of Seismically Active Parts of Underground Faults via Seismic Data Mining

    NASA Astrophysics Data System (ADS)

    Frantzeskakis, Theofanis; Konstantaras, Anthony

    2015-04-01

    During the last few years rapid steps have been taken towards drilling for oil in the western Mediterranean sea. Since most of the countries in the region benefit mainly from tourism and considering that the Mediterranean is a closed sea only replenishing its water once every ninety years careful measures are being taken to ensure safe drilling. In that concept this research work attempts to derive a three dimensional model of the seismically active parts of the underlying underground faults in areas of petroleum interest. For that purpose seismic spatio-temporal clustering has been applied to seismic data to identify potential distinct seismic regions in the area of interest. Results have been coalesced with two dimensional maps of underground faults from past surveys and seismic epicentres, having followed careful reallocation processing, have been used to provide information regarding the vertical extent of multiple underground faults in the region of interest. The end product is a three dimensional map of the possible underground location and extent of the seismically active parts of underground faults. Indexing terms: underground faults modelling, seismic data mining, 3D visualisation, active seismic source mapping, seismic hazard evaluation, dangerous phenomena modelling Acknowledgment This research work is supported by the ESPA Operational Programme, Education and Life Long Learning, Students Practical Placement Initiative. References [1] Alves, T.M., Kokinou, E. and Zodiatis, G.: 'A three-step model to assess shoreline and offshore susceptibility to oil spills: The South Aegean (Crete) as an analogue for confined marine basins', Marine Pollution Bulletin, In Press, 2014 [2] Ciappa, A., Costabile, S.: 'Oil spill hazard assessment using a reverse trajectory method for the Egadi marine protected area (Central Mediterranean Sea)', Marine Pollution Bulletin, vol. 84 (1-2), pp. 44-55, 2014 [3] Ganas, A., Karastathis, V., Moshou, A., Valkaniotis, S., Mouzakiotis

  17. Stereo Pair: Basalt Cliffs, Patagonia, Argentina

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Basalt cliffs along the northwest edge of the Meseta de Somuncura plateau near Sierra Colorada, Argentina show an unusual and striking pattern of erosion. Stereoscopic observation helps to clarify the landform changing processes active here. Many of the cliffs appear to be rock staircases that have the same color as the plateau's basaltic cap rock. Are these the edges of lower layers in the basalt or are they a train of slivers that are breaking off from, then sliding downslope and away from, the cap rock. They appear to be the latter. Close inspection shows that each stair step is too laterally irregular to be a continuous sheet of bedrock like the cap rock. Also, the steps are not flat but instead are little ridges, as one might expect from broken, tilted, and sliding slices of the cap rock. Stream erosion has cut some gullies into the cliffs and green vegetation shows that water springs from and flows down some channels, but landsliding is clearly a major agent of erosion here.

    This cross-eyed stereoscopic image pair was generated using topographic data from the Shuttle Radar Topography Mission, combined with an enhanced Landsat 7satellite color image. The topography data are used to create two differing perspectives of a single image, one perspective for each eye. In doing so, each point in the image is shifted slightly, depending on its elevation. When stereoscopically merged, the result is a vertically exaggerated view of the Earth's surface in its full three dimensions.

    Landsat satellites have provided visible light and infrared images of the Earth continuously since 1972. SRTM topographic data match the 30-meter (99-foot) spatial resolution of most Landsat images and provide a valuable complement for studying the historic and growing Landsat data archive. The Landsat 7 Thematic Mapper image used here was provided to the SRTM project by the United States Geological Survey, Earth Resources Observation Systems (EROS) Data Center,Sioux Falls, South

  18. 3D and Education

    NASA Astrophysics Data System (ADS)

    Meulien Ohlmann, Odile

    2013-02-01

    Today the industry offers a chain of 3D products. Learning to "read" and to "create in 3D" becomes an issue of education of primary importance. 25 years professional experience in France, the United States and Germany, Odile Meulien set up a personal method of initiation to 3D creation that entails the spatial/temporal experience of the holographic visual. She will present some different tools and techniques used for this learning, their advantages and disadvantages, programs and issues of educational policies, constraints and expectations related to the development of new techniques for 3D imaging. Although the creation of display holograms is very much reduced compared to the creation of the 90ies, the holographic concept is spreading in all scientific, social, and artistic activities of our present time. She will also raise many questions: What means 3D? Is it communication? Is it perception? How the seeing and none seeing is interferes? What else has to be taken in consideration to communicate in 3D? How to handle the non visible relations of moving objects with subjects? Does this transform our model of exchange with others? What kind of interaction this has with our everyday life? Then come more practical questions: How to learn creating 3D visualization, to learn 3D grammar, 3D language, 3D thinking? What for? At what level? In which matter? for whom?

  19. Computer-aided diagnosis of pulmonary nodules on CT scans: Segmentation and classification using 3D active contours

    PubMed Central

    Way, Ted W.; Hadjiiski, Lubomir M.; Sahiner, Berkman; Chan, Heang-Ping; Cascade, Philip N.; Kazerooni, Ella A.; Bogot, Naama; Zhou, Chuan

    2009-01-01

    We are developing a computer-aided diagnosis (CAD) system to classify malignant and benign lung nodules found on CT scans. A fully automated system was designed to segment the nodule from its surrounding structured background in a local volume of interest (VOI) and to extract image features for classification. Image segmentation was performed with a three-dimensional (3D) active contour (AC) method. A data set of 96 lung nodules (44 malignant, 52 benign) from 58 patients was used in this study. The 3D AC model is based on two-dimensional AC with the addition of three new energy components to take advantage of 3D information: (1) 3D gradient, which guides the active contour to seek the object surface, (2) 3D curvature, which imposes a smoothness constraint in the z direction, and (3) mask energy, which penalizes contours that grow beyond the pleura or thoracic wall. The search for the best energy weights in the 3D AC model was guided by a simplex optimization method. Morphological and gray-level features were extracted from the segmented nodule. The rubber band straightening transform (RBST) was applied to the shell of voxels surrounding the nodule. Texture features based on run-length statistics were extracted from the RBST image. A linear discriminant analysis classifier with stepwise feature selection was designed using a second simplex optimization to select the most effective features. Leave-one-case-out resampling was used to train and test the CAD system. The system achieved a test area under the receiver operating characteristic curve (Az) of 0.83±0.04. Our preliminary results indicate that use of the 3D AC model and the 3D texture features surrounding the nodule is a promising approach to the segmentation and classification of lung nodules with CAD. The segmentation performance of the 3D AC model trained with our data set was evaluated with 23 nodules available in the Lung Image Database Consortium (LIDC). The lung nodule volumes segmented by the 3D AC

  20. Foreign Language Vocabulary Development through Activities in an Online 3D Environment

    ERIC Educational Resources Information Center

    Milton, James; Jonsen, Sunniva; Hirst, Steven; Lindenburn, Sharn

    2012-01-01

    On-line virtual 3D worlds offer the opportunity for users to interact in real time with native speakers of the language they are learning. In principle, this ought to be of great benefit to learners, and mimicking the opportunity for immersion that real-life travel to a foreign country offers. We have very little research to show whether this is…

  1. Morphological analysis of waterfall cliff face by terrestrial laser scanning

    NASA Astrophysics Data System (ADS)

    Hayakawa, Y. S.

    2012-04-01

    A waterfall highlights the locus of active erosion in bedrock rivers around mountainous and plateau areas. Mechanism of bedrock erosion at a waterfall has been studied for some cases, but there remain uncertainties in erosional processes with regard to detailed form of rocks composing a waterfall. In this study the details of cliff forms around a waterfall is examined using a terrestrial laser scanner in relation to processes occurring in the cliff at and around the waterfall. The study site is Kegon Falls in Japan, having a 97-m high vertical drop of surface water with outflows of underground water at the lower portion of the cliff. Since the cliff is vertical to overhanging, a DEM is created against a vertical plane, and longitudinal and transverse profiles extracted from the DEM are investigated. Stability analysis of the waterfall cliff indicates that the igneous rock composing the cliff is strong enough to keep its current overhanging shape, and catastrophic collapse of whole of the waterfall face seems to hardly occur. Indeed, the upper cliff face is being gradually eroded by gravitational collapses of blocks bounded by joints, which may occur once in decades. The most recent large collapse of the upper cliff occurred in 1986, which caused recession of the waterfall for approximately 10 m, whereas smaller collapses of the cliff seem to have occurred more frequently. The erosion by surface water sets the position of horizontal depression of the cliff top, while underground outflow plays a prior role in undercutting the upper cliff base. Although the outflows of underground water coming from an upstream lake may not freezes much due to its moderate temperature, freeze-thaw process frequently occurs on cliff surfaces around the waterfall due to sprays mostly from the surface water (and partly from the underground water outflow) in winter to spring seasons. The jointed rocks thus seem to be gradually weakened to cause rockfalls, particularly at the middle portion

  2. A Facile Synthesis, In vitro Antiinflammatory and Antioxidant activity of Novel Benzimidazolylpyrano[2,3-d][1,3]thiazolocarbonitriles

    PubMed Central

    Malladi, S. R.; Anisetti, R.; Rao, P. R.

    2014-01-01

    The synthesis benzimidazolylpyrano [2,3-d] [1,3] thiazolocarbonitriles (5a-j) were achieved by cyclocondensation of arylidene amino-benzo[d]imidazole-2-thiols (3a-j) with mercaptoacetic acid followed by cyclization with 2-(phenylmethylene)malononitrile. Further more, the present study aimed at the evaluation of in vitro antiinflammatory activity and antioxidant activity of synthetic compounds. All tested compounds showed appreciable activity against the standard drugs. PMID:25593384

  3. Novel implementations of optical switch control module and 3D-CSP for 10 Gbps active optical access system

    NASA Astrophysics Data System (ADS)

    Wakayama, Koji; Okuno, Michitaka; Matsuoka, Yasunobu; Hosomi, Kazuhiko; Sagawa, Misuzu; Sugawara, Toshiki

    2009-11-01

    We propose an optical switch control procedure for high-performance and cost-effective 10 Gbps Active Optical Access System (AOAS) in which optical switches are used instead of optical splitters in PON (Passive Optical Network). We demonstrate the implemented optical switch control module on Optical Switching Unit (OSW) with logic circuits works effectively. We also propose a compact optical 3D-CSP (Chip Scale Package) to achieve the high performance of AOAS without losing cost advantage of PON. We demonstrate the implemented 3D-CSP works effectively.

  4. A Comparative Analysis between Active and Passive Techniques for Underwater 3D Reconstruction of Close-Range Objects

    PubMed Central

    Bianco, Gianfranco; Gallo, Alessandro; Bruno, Fabio; Muzzupappa, Maurizio

    2013-01-01

    In some application fields, such as underwater archaeology or marine biology, there is the need to collect three-dimensional, close-range data from objects that cannot be removed from their site. In particular, 3D imaging techniques are widely employed for close-range acquisitions in underwater environment. In this work we have compared in water two 3D imaging techniques based on active and passive approaches, respectively, and whole-field acquisition. The comparison is performed under poor visibility conditions, produced in the laboratory by suspending different quantities of clay in a water tank. For a fair comparison, a stereo configuration has been adopted for both the techniques, using the same setup, working distance, calibration, and objects. At the moment, the proposed setup is not suitable for real world applications, but it allowed us to conduct a preliminary analysis on the performances of the two techniques and to understand their capability to acquire 3D points in presence of turbidity. The performances have been evaluated in terms of accuracy and density of the acquired 3D points. Our results can be used as a reference for further comparisons in the analysis of other 3D techniques and algorithms. PMID:23966193

  5. A comparative analysis between active and passive techniques for underwater 3D reconstruction of close-range objects.

    PubMed

    Bianco, Gianfranco; Gallo, Alessandro; Bruno, Fabio; Muzzupappa, Maurizio

    2013-01-01

    In some application fields, such as underwater archaeology or marine biology, there is the need to collect three-dimensional, close-range data from objects that cannot be removed from their site. In particular, 3D imaging techniques are widely employed for close-range acquisitions in underwater environment. In this work we have compared in water two 3D imaging techniques based on active and passive approaches, respectively, and whole-field acquisition. The comparison is performed under poor visibility conditions, produced in the laboratory by suspending different quantities of clay in a water tank. For a fair comparison, a stereo configuration has been adopted for both the techniques, using the same setup, working distance, calibration, and objects. At the moment, the proposed setup is not suitable for real world applications, but it allowed us to conduct a preliminary analysis on the performances of the two techniques and to understand their capability to acquire 3D points in presence of turbidity. The performances have been evaluated in terms of accuracy and density of the acquired 3D points. Our results can be used as a reference for further comparisons in the analysis of other 3D techniques and algorithms. PMID:23966193

  6. A motion- and sound-activated, 3D-printed, chalcogenide-based triboelectric nanogenerator.

    PubMed

    Kanik, Mehmet; Say, Mehmet Girayhan; Daglar, Bihter; Yavuz, Ahmet Faruk; Dolas, Muhammet Halit; El-Ashry, Mostafa M; Bayindir, Mehmet

    2015-04-01

    A multilayered triboelectric nanogenerator (MULTENG) that can be actuated by acoustic waves, vibration of a moving car, and tapping motion is built using a 3D-printing technique. The MULTENG can generate an open-circuit voltage of up to 396 V and a short-circuit current of up to 1.62 mA, and can power 38 LEDs. The layers of the triboelectric generator are made of polyetherimide nanopillars and chalcogenide core-shell nanofibers. PMID:25722118

  7. 3D Traction Stresses Activate Protease-Dependent Invasion of Cancer Cells

    PubMed Central

    Aung, Aereas; Seo, Young N.; Lu, Shaoying; Wang, Yingxiao; Jamora, Colin; del Álamo, Juan C.; Varghese, Shyni

    2014-01-01

    Cell invasion and migration that occurs, for example, in cancer metastasis is rooted in the ability of cells to navigate through varying levels of physical constraint exerted by the extracellular matrix. Cancer cells can invade matrices in either a protease-independent or a protease-dependent manner. An emerging critical component that influences the mode of cell invasion is the traction stresses generated by the cells in response to the physicostructural properties of the extracellular matrix. In this study, we have developed a reference-free quantitative assay for measuring three-dimensional (3D) traction stresses generated by cells during the initial stages of invasion into matrices exerting varying levels of mechanical resistance. Our results show that as cells encounter higher mechanical resistance, a larger fraction of them shift to protease-mediated invasion, and this process begins at lower values of cell invasion depth. On the other hand, the compressive stress generated by the cells at the onset of protease-mediated invasion is found to be independent of matrix stiffness, suggesting that 3D traction stress is a key factor in triggering protease-mediated cancer cell invasion. At low 3D compressive traction stresses, cells utilize bleb formation to indent the matrix in a protease independent manner. However, at higher stress values, cells utilize invadopodia-like structures to mediate protease-dependent invasion into the 3D matrix. The critical value of compressive traction stress at the transition from a protease-independent to a protease-dependent mode of invasion is found to be ∼165 Pa. PMID:25468332

  8. Estimating 3D variation in active-layer thickness beneath arctic streams using ground-penetrating radar

    USGS Publications Warehouse

    Brosten, T.R.; Bradford, J.H.; McNamara, J.P.; Gooseff, M.N.; Zarnetske, J.P.; Bowden, W.B.; Johnston, M.E.

    2009-01-01

    We acquired three-dimensional (3D) ground-penetrating radar (GPR) data across three stream sites on the North Slope, AK, in August 2005, to investigate the dependence of thaw depth on channel morphology. Data were migrated with mean velocities derived from multi-offset GPR profiles collected across a stream section within each of the 3D survey areas. GPR data interpretations from the alluvial-lined stream site illustrate greater thaw depths beneath riffle and gravel bar features relative to neighboring pool features. The peat-lined stream sites indicate the opposite; greater thaw depths beneath pools and shallower thaw beneath the connecting runs. Results provide detailed 3D geometry of active-layer thaw depths that can support hydrological studies seeking to quantify transport and biogeochemical processes that occur within the hyporheic zone.

  9. Active illumination based 3D surface reconstruction and registration for image guided medialization laryngoplasty

    NASA Astrophysics Data System (ADS)

    Jin, Ge; Lee, Sang-Joon; Hahn, James K.; Bielamowicz, Steven; Mittal, Rajat; Walsh, Raymond

    2007-03-01

    The medialization laryngoplasty is a surgical procedure to improve the voice function of the patient with vocal fold paresis and paralysis. An image guided system for the medialization laryngoplasty will help the surgeons to accurately place the implant and thus reduce the failure rates of the surgery. One of the fundamental challenges in image guided system is to accurately register the preoperative radiological data to the intraoperative anatomical structure of the patient. In this paper, we present a combined surface and fiducial based registration method to register the preoperative 3D CT data to the intraoperative surface of larynx. To accurately model the exposed surface area, a structured light based stereo vision technique is used for the surface reconstruction. We combined the gray code pattern and multi-line shifting to generate the intraoperative surface of the larynx. To register the point clouds from the intraoperative stage to the preoperative 3D CT data, a shape priori based ICP method is proposed to quickly register the two surfaces. The proposed approach is capable of tracking the fiducial markers and reconstructing the surface of larynx with no damage to the anatomical structure. We used off-the-shelf digital cameras, LCD projector and rapid 3D prototyper to develop our experimental system. The final RMS error in the registration is less than 1mm.

  10. Activation of the marine ecosystem model 3D CEMBS for the Baltic Sea in operational mode

    NASA Astrophysics Data System (ADS)

    Dzierzbicka-Glowacka, Lidia; Jakacki, Jaromir; Janecki, Maciej; Nowicki, Artur

    2013-04-01

    The paper presents a new marine ecosystem model 3D CEMBS designed for the Baltic Sea. The ecosystem model is incorporated into the 3D POPCICE ocean-ice model. The Current Baltic Sea model is based on the Community Earth System Model (CESM from the National Center for Atmospheric Research) which was adapted for the Baltic Sea as a coupled sea-ice model. It consists of the Community Ice Code (CICE model, version 4.0) and the Parallel Ocean Program (version 2.1). The ecosystem model is a biological submodel of the 3D CEMBS. It consists of eleven mass conservation equations. There are eleven partial second-order differential equations of the diffusion type with the advective term for phytoplankton, zooplankton, nutrients, dissolved oxygen, and dissolved and particulate organic matter. This model is an effective tool for solving the problem of ecosystem bioproductivity. The model is forced by 48-hour atmospheric forecasts provided by the UM model from the Interdisciplinary Centre for Mathematical and Computational Modelling of Warsaw University (ICM). The study was financially supported by the Polish State Committee of Scientific Research (grants: No N N305 111636, N N306 353239). The partial support for this study was also provided by the project Satellite Monitoring of the Baltic Sea Environment - SatBaltyk founded by European Union through European Regional Development Fund contract no. POIG 01.01.02-22-011/09. Calculations were carried out at the Academy Computer Centre in Gdańsk.

  11. White Cliffs: Operating Experience

    NASA Technical Reports Server (NTRS)

    Kaneff, S.

    1984-01-01

    The fourteen dish white cliffs solar power station area is remote and subject to extreme environmental conditions, solution of the associated problems required careful and thoughtful attention and the application of resources. Notwithstanding the wide range and harshness of conditions, the difficulties caused by remoteness and the lack of a technological base and the need for relatively rapid demonstration of success, the project has had a very positive outcome. Qualitative and quantitative information and lessons are now available to enable considerable simplifications to be made for a new system, reducing both hardware and operation and maintenance costs. Experience and lessons are presented, particularly in relation to: system performance in various environmental conditions; design philosophies for collectors, the array, control systems, engine and plant; operation and maintenance strategies and cost reducing possibilities. Experience so far gives encouragement for the future of such paraboloidal dish systems in appropriate areas.

  12. North Polar Cliff

    NASA Technical Reports Server (NTRS)

    2006-01-01

    This full HiRISE image shows a cliff-face that has been eroded into the ice-rich polar layered deposits at the head of the large canyon, Chasma Boreale. In a similar way to layers in the Earth's ice caps, these Martian layers are thought to record variations in climate, which makes them very interesting to scientists. This particular cliff-face is several hundred meters high and the layers exposed here are the deepest (and so the oldest) in the polar layered deposits. The lower layers exposed in this scarp appear to be rich in dark sand, and erosion of these layers has produced the sand dunes that cover sections of this cliff-face. A close examination of the layers in the center of the image shows they have curved shapes and intersect each other. Scientists call this cross-bedding and it may indicate that these sandy layers were laid down as a large dunefield before being buried. At the bottom of the image, the floor of Chasma Boreale in this area appears to have been swept clean of sandy material. There is a complex history of erosion and deposition of material at this location. On the right of the image one can see a smooth material that covers the lower layers and which must have been deposited after the main cliff face was initially eroded. Closer to the center of the image, this smooth mantling material is in turn being eroded away to once again expose the layers beneath it.

    Image PSP_001334_2645 was taken by the High Resolution Imaging Science Experiment (HiRISE) camera onboard the Mars Reconnaissance Orbiter spacecraft on November 8, 2006. The complete image is centered at 84.4 degrees latitude, 343.5 degrees East longitude. The range to the target site was 317.4 km (198.4 miles). At this distance the image scale ranges from 31.8 cm/pixel (with 1 x 1 binning) to 63.5 cm/pixel (with 2 x 2 binning). The image shown here has been map-projected to 25 cm/pixel. The image was taken at a local Mars time of 1:38 PM and the scene is illuminated from the

  13. Active and Passive 3d Imaging Technologies Applied to Waterlogged Wooden Artifacts from Shipwrecks

    NASA Astrophysics Data System (ADS)

    Bandiera, A.; Alfonso, C.; Auriemma, R.

    2015-04-01

    The fragility of organic artefacts in presence of water and their volumetric variation caused by the marine life on or surrounding them dictate that their physical dimensions be measured soon after their extraction from the seabed. In an ideal context, it would be appropriate to preserve and restore all the archaeological elements, rapidly and with the latest methods. Unfortunately however, the large number of artefacts makes the cost of such an operation prohibitive for a public institution. For this reason, digital technologies for documentation, restoration, display and conservation are being considered by many institutions working with limited budgets. In this paper, we illustrate the experience of the University of Salento in 3D imaging technology for waterlogged wooden artefacts from shipwrecks. The interest originates from the need to develop a protocol for documentation and digital restoration of archaeological finds discovered along the coast of Torre S. Sabina (BR) Italy. This work has allowed us to explore recent technologies for 3D acquisitions, both underwater and in the laboratory, as well as methods for data processing. These technologies have permitted us to start defining a protocol to follow for all waterlogged wooden artefacts requiring documentation and restoration.

  14. tomo3d: a new 3-D joint refraction and reflection travel-time tomography code for active-source seismic data

    NASA Astrophysics Data System (ADS)

    Meléndez, A.; Korenaga, J.; Sallares, V.; Ranero, C. R.

    2012-12-01

    We present the development state of tomo3d, a code for three-dimensional refraction and reflection travel-time tomography of wide-angle seismic data based on the previous two-dimensional version of the code, tomo2d. The core of both forward and inverse problems is inherited from the 2-D version. The ray tracing is performed by a hybrid method combining the graph and bending methods. The graph method finds an ordered array of discrete model nodes, which satisfies Fermat's principle, that is, whose corresponding travel time is a global minimum within the space of discrete nodal connections. The bending method is then applied to produce a more accurate ray path by using the nodes as support points for an interpolation with beta-splines. Travel time tomography is formulated as an iterative linearized inversion, and each step is solved using an LSQR algorithm. In order to avoid the singularity of the sensitivity kernel and to reduce the instability of inversion, regularization parameters are introduced in the inversion in the form of smoothing and damping constraints. Velocity models are built as 3-D meshes, and velocity values at intermediate locations are obtained by trilinear interpolation within the corresponding pseudo-cubic cell. Meshes are sheared to account for topographic relief. A floating reflector is represented by a 2-D grid, and depths at intermediate locations are calculated by bilinear interpolation within the corresponding square cell. The trade-off between the resolution of the final model and the associated computational cost is controlled by the relation between the selected forward star for the graph method (i.e. the number of nodes that each node considers as its neighbors) and the refinement of the velocity mesh. Including reflected phases is advantageous because it provides a better coverage and allows us to define the geometry of those geological interfaces with velocity contrasts sharp enough to be observed on record sections. The code also

  15. Thermal impact of an active 3-D microelectrode array implanted in the brain.

    PubMed

    Kim, Sohee; Tathireddy, Prashant; Normann, Richard A; Solzbacher, Florian

    2007-12-01

    A chronically implantable, wireless neural interface device will require integrating electronic circuitry with the interfacing microelectrodes in order to eliminate wired connections. Since the integrated circuit (IC) dissipates a certain amount of power, it will raise the temperature in surrounding tissues where it is implanted. In this paper, the thermal influence of the integrated 3-D Utah electrode array (UEA) device implanted in the brain was investigated by numerical simulation using finite element analysis (FEA) and by experimental measurement in vitro as well as in vivo. The numerically calculated and experimentally measured temperature increases due to the UEA implantation were in good agreement. The experimentally validated numerical model predicted that the temperature increases linearly with power dissipation through the UEA, with a slope of 0.029 degree C/mW over the power dissipation levels expected to be used. The influences of blood perfusion, brain metabolism, and UEA geometry on tissue heating were also investigated using the numerical model. PMID:18198706

  16. Additive manufactured polymeric 3D scaffolds with tailored surface topography influence mesenchymal stromal cells activity.

    PubMed

    Neves, Sara C; Mota, Carlos; Longoni, Alessia; Barrias, Cristina C; Granja, Pedro L; Moroni, Lorenzo

    2016-06-01

    Additive manufactured three-dimensional (3D) scaffolds with tailored surface topography constitute a clear advantage in tissue regeneration strategies to steer cell behavior. 3D fibrous scaffolds of poly(ethylene oxide terephthalate)/poly(butylene terephthalate) block copolymer presenting different fiber surface features were successfully fabricated by additive manufacturing combined with wet-spinning, in a single step, without any post-processing. The optimization of the processing parameters, mainly driven by different solvent/non-solvent combinations, led to four distinct scaffold types, with average surface roughness values ranging from 0.071 ± 0.012 μm to 1.950 ± 0.553 μm, average pore sizes in the x- and y-axis between 351.1 ± 33.6 μm and 396.1 ± 32.3 μm, in the z-axis between 36.5 ± 5.3 μm and 70.7 ± 8.8 μm, average fiber diameters between 69.4 ± 6.1 μm and 99.0 ± 9.4 μm, and porosity values ranging from 60.2 ± 0.8% to 71.7 ± 2.6%. Human mesenchymal stromal cells (hMSCs) cultured on these scaffolds adhered, proliferated, and produced endogenous extracellular matrix. The effect of surface roughness and topography on hMSCs differentiation was more evident for cells seeded at lower density, where the percentage of cells in direct contact with the surface was higher compared to more densely seeded scaffolds. Under osteogenic conditions, lower surface roughness values (0.227 ± 0.035 μm) had a synergistic effect on hMSCs behavior, while chondrogenesis was favored on rougher surfaces (1.950 ± 0.553 μm). PMID:27219645

  17. Unique characteristics of human mesenchymal stem/progenitor cells (MSC) pre-activated in 3D cultures under different conditions

    PubMed Central

    Ylostalo, Joni H.; Bartosh, Thomas J.; Tiblow, April; Prockop, Darwin J.

    2014-01-01

    Background Human mesenchymal stem cells (MSCs) are being employed in clinical trials, but the best protocol to prepare the cells for administration to patients remains unclear. We previously demonstrated that MSCs could be pre-activated to express therapeutic factors by culturing the cells in 3D. Here we compared the activation of MSCs in 3D in fetal bovine serum (FBS) containing medium and in multiple xeno-free media formulations. Methods MSC aggregation and sphere formation was studied using hanging drop cultures with medium containing FBS or with various commercially available stem cell media with or without human serum albumin (HSA). Activation of MSCs was studied with gene expression and protein secretion measurements and with functional studies using macrophages and cancer cells. Results MSCs did not condense into tight spheroids and express a full complement of therapeutic genes in MEMα or several commercial stem-cell media. However, we identified a chemically-defined xeno-free media that when supplemented with HSA from blood or recombinant HSA, resulted in compact spheres with high cell viability, together with high expression of anti-inflammatory (PGE2, TSG-6) and anti-cancer molecules (TRAIL, IL-24). Furthermore, spheres cultured in this medium showed potent anti-inflammatory effects in an LPS-stimulated macrophage system, and suppressed the growth of prostate cancer cells by promoting cell-cycle arrest and cell death. Discussion We demonstrated that cell activation in 3D depends critically on the culture medium. The conditions developed here for 3D culture of MSCs should be useful in further research on MSCs and their potential therapeutic applications. PMID:25231893

  18. tomo3d: a new 3-D joint refraction and reflection travel-time tomography code for active-source seismic data

    NASA Astrophysics Data System (ADS)

    Meléndez, A.; Korenaga, J.; Sallarès, V.; Ranero, C. R.

    2012-04-01

    We present the development state of tomo3d, a code for three-dimensional refraction and reflection travel-time tomography of wide-angle seismic data based on the previous two-dimensional version of the code, tomo2d. The core of both forward and inverse problems is inherited from the 2-D version. The ray tracing is performed by a hybrid method combining the graph and bending methods. The graph method finds an ordered array of discrete model nodes, which satisfies Fermat's principle, that is, whose corresponding travel time is a global minimum within the space of discrete nodal connections. The bending method is then applied to produce a more accurate ray path by using the nodes as support points for an interpolation with beta-splines. Travel time tomography is formulated as an iterative linearized inversion, and each step is solved using an LSQR algorithm. In order to avoid the singularity of the sensitivity kernel and to reduce the instability of inversion, regularization parameters are introduced in the inversion in the form of smoothing and damping constraints. Velocity models are built as 3-D meshes, and velocity values at intermediate locations are obtained by trilinear interpolation within the corresponding pseudo-cubic cell. Meshes are sheared to account for topographic relief. A floating reflector is represented by a 2-D grid, and depths at intermediate locations are calculated by bilinear interpolation within the corresponding square cell. The trade-off between the resolution of the final model and the associated computational cost is controlled by the relation between the selected forward star for the graph method (i.e. the number of nodes that each node considers as its neighbors) and the refinement of the velocity mesh. Including reflected phases is advantageous because it provides a better coverage and allows us to define the geometry of those geological interfaces with velocity contrasts sharp enough to be observed on record sections. The code also

  19. Sea cliff instability susceptibility at regional scale: A statistically based assessment in southern Algarve, Portugal.

    NASA Astrophysics Data System (ADS)

    Marques, F.; Matildes, R.; Redweik, P.

    2012-04-01

    Mass movements are the dominant process of sea cliff evolution, being a considerable source of natural hazard and a significant constrain for human activities in coastal areas. Related hazards include cliff top retreat, with implications on planning and land management, and unstable soil or rock movements at the cliffs face and toe, with implications mainly on beach users and support structures. To assess the spatial component of sea cliff hazard assessment with implications on planning, i.e. the susceptibility of a given cliff section to be affected by instabilities causing retreat of the cliff top, a statistically based study was carried out along the top of the sea cliffs of Burgau-Lagos coastal section (Southwest Algarve, Portugal). The study was based on bivariate and multi-variate statistics applied to a set of predisposing factors, mainly related with geology and geomorphology, which were correlated with an inventory of past cliff failures. The multi-temporal inventory of past cliff failures was produced using aerial digital photogrammetric methods, which included special procedures to enable the extraction of accurate data from old aerial photos, and validated by systematic stereo photo interpretation, helped by oblique aerial photos and field surveys. This study identified 137 cliff failures occurred between 1947 and 2007 along the 13 km long cliffs, causing the loss of 10,234 m2 of horizontal area at the cliffs top. The cliff failures correspond to planar slides (58%) mainly in Cretaceous alternating limestone and marls, toppling failures (17%) mainly in Miocene calcarenites, slumps (15%) in Plio-pleistocene silty sands that infill the karst in the Miocene rocks, and the remaining 10% correspond to complex movements, rockfalls and not determined cases. The space distribution of cliff failures is quite irregular but enables the objective separation of sub sections with homogeneous retreat behavior, for which were computed mean retreat rates between 5x10-3m

  20. Self-assembled 3D hierarchical sheaf-like Nb3O7(OH) nanostructures with enhanced photocatalytic activity

    NASA Astrophysics Data System (ADS)

    Hu, Pei; Hou, Dongfang; Wen, Yanwei; Shan, Bin; Chen, Chaoji; Huang, Yunhui; Hu, Xianluo

    2015-01-01

    Novel three-dimensional (3D) hierarchical Nb3O7(OH) nanostructures with a sheaf-like nanoarchitecture were fabricated for the first time by a hydrothermal process. Interestingly, the nanosheafs are composed of nanorods with an average diameter of about 25 nm. The as-prepared 3D hierarchical nanostructures possess a high surface area of 77 m2 g-1 with pore diameters of ca. 4.2-12.5 nm. A possible growth mechanism based on the combined Ostwald ripening and self-assembly process was proposed. It is found that both the valence-band top and the conduction-band bottom consist of O 2p and Nb 4d orbitals. Importantly, the 3D hierarchical Nb3O7(OH) nanostructures exhibit enhanced photocatalytic activity for the degradation of Rhodamine B (RhB) under UV-visible light, which is attributed to the unusual hierarchical structure, high surface area, and hybridization of energy bands.Novel three-dimensional (3D) hierarchical Nb3O7(OH) nanostructures with a sheaf-like nanoarchitecture were fabricated for the first time by a hydrothermal process. Interestingly, the nanosheafs are composed of nanorods with an average diameter of about 25 nm. The as-prepared 3D hierarchical nanostructures possess a high surface area of 77 m2 g-1 with pore diameters of ca. 4.2-12.5 nm. A possible growth mechanism based on the combined Ostwald ripening and self-assembly process was proposed. It is found that both the valence-band top and the conduction-band bottom consist of O 2p and Nb 4d orbitals. Importantly, the 3D hierarchical Nb3O7(OH) nanostructures exhibit enhanced photocatalytic activity for the degradation of Rhodamine B (RhB) under UV-visible light, which is attributed to the unusual hierarchical structure, high surface area, and hybridization of energy bands. Electronic supplementary information (ESI) available: XRD patterns, XPS spectra, and SEM images. See DOI: 10.1039/c4nr06580h

  1. Production of Uniform 3D Microtumors in Hydrogel Microwell Arrays for Measurement of Viability, Morphology, and Signaling Pathway Activation.

    PubMed

    Singh, Manjulata; Close, David A; Mukundan, Shilpaa; Johnston, Paul A; Sant, Shilpa

    2015-11-01

    Despite significant investments in cancer research and drug discovery/development, the rate of new cancer drug approval is ≤5% and most cases of metastatic cancer remain incurable. Ninety-five percent of new cancer drugs fail in clinical development because of a lack of therapeutic efficacy and/or unacceptable toxicity. One of the major factors responsible for the low success rate of anticancer drug development is the failure of preclinical models to adequately recapitulate the complexity and heterogeneity of human cancer. For throughput and capacity reasons, high-throughput screening growth inhibition assays almost exclusively use two-dimensional (2D) monolayers of tumor cell lines cultured on tissue culture-treated plastic/glass surfaces in serum-containing medium. However, these 2D tumor cell line cultures fail to recapitulate the three-dimensional (3D) context of cells in solid tumors even though the tumor microenvironment has been shown to have a profound effect on anticancer drug responses. Tumor spheroids remain the best characterized and most widely used 3D models; however, spheroid sizes tend to be nonuniform, making them unsuitable for high-throughput drug testing. To circumvent this challenge, we have developed defined size microwell arrays using nonadhesive hydrogels that are applicable to a wide variety of cancer cell lines to fabricate size-controlled 3D microtumors. We demonstrate that the hydrogel microwell array platform can be applied successfully to generate hundreds of uniform microtumors within 3-6 days from many cervical and breast, as well as head and neck squamous cell carcinoma (HNSCC) cells. Moreover, controlling size of the microwells in the hydrogel array allows precise control over the size of the microtumors. Finally, we demonstrate the application of this platform technology to probe activation as well as inhibition of epidermal growth factor receptor (EGFR) signaling in 3D HNSCC microtumors in response to EGF and cetuximab

  2. Carbon deposition from aromatic solvents onto active intact 3d metal surface at ambient conditions.

    PubMed

    Safronov, A P; Kurlyandskaya, G V; Chlenova, A A; Kuznetsov, M V; Bazhin, D N; Beketov, I V; Sanchez-Ilarduya, M B; Martinez-Amesti, A

    2014-03-25

    The process of carbon deposition onto 3d metal surface immersed in aromatic solvents (benzene, toluene, xylene) at ambient conditions was studied for as-prepared magnetic nanoparticles (MNPs) and Fe-based films by thermal analysis, mass spectrometry, X-ray diffraction, X-ray photoelectron spectroscopy, Raman spectroscopy, electron microscopy, and energy-dispersive X-ray spectroscopy. The mechanism of the deposition at the interface is likely the heterogeneous Scholl oxidation of the aromatic hydrocarbons, which is the cationic polymerization of the aryl rings. It results in the formation of polycyclic aromatic hydrocarbons (PAH) chemically bonded to the surface of a MNP or thin metallic film. The benzene rings in the polycyclic deposit do not maintain planar aligned structures and do not provide delocalization of the π-electrons in the zone structure. Contrary to the dense graphite layers, the polycyclic layers, although chemically bonded, are not attached tightly to the surface. Such "hairlike" structure of the carboneous deposit might be especially favorable for the applications that imply the enhanced interaction at the surfaces incorporated in the functional matrices (polymeric composites or biosensors). The aromatic chemical nature of the deposit provides strong interaction with most polymers, while its loose structure favors conformational mobility of macromolecular chains at the interface. PMID:24593324

  3. 3D hierarchical walnut-like CuO nanostructures: Preparation, characterization and their efficient catalytic activity for CO oxidation

    NASA Astrophysics Data System (ADS)

    Yao, Weitang; Zhang, Yujuan; Duan, Tao; Zhu, Wenkun; Yi, Zao; Cui, Xudong

    2016-07-01

    In this work, 3D hierarchical walnut-shaped, 2D nanosheet and 3D microspheres single phase CuO nanostructures are functioning as catalysts and supporting materials, differing from the conventional ways. The novel nanostructures were synthesized via hydrothermal method under a stainless steel autoclave. The as-prepared materials were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), and H2 temperature-programmed reduction (H2-TPR). The walnut-shaped structures with high O/Cu atomic ratio (1.22) exhibit high oxygen adsorption capacity and greatly enhanced catalytic activity. These results will be enrich the techniques for tuning the morphologies of metal oxide micro/nanostructures and open a new field in catalytic applications.

  4. Comparative velocity structure of active Hawaiian volcanoes from 3-D onshore-offshore seismic tomography

    USGS Publications Warehouse

    Park, J.; Morgan, J.K.; Zelt, C.A.; Okubo, P.G.; Peters, L.; Benesh, N.

    2007-01-01

    We present a 3-D P-wave velocity model of the combined subaerial and submarine portions of the southeastern part of the Island of Hawaii, based on first-arrival seismic tomography of marine airgun shots recorded by the onland seismic network. Our model shows that high-velocity materials (6.5-7.0??km/s) lie beneath Kilauea's summit, Koae fault zone, and the upper Southwest Rift Zone (SWRZ) and upper and middle East Rift Zone (ERZ), indicative of magma cumulates within the volcanic edifice. A separate high-velocity body of 6.5-6.9??km/s within Kilauea's lower ERZ and upper Puna Ridge suggests a distinct body of magma cumulates, possibly connected to the summit magma cumulates at depth. The two cumulate bodies within Kilauea's ERZ may have undergone separate ductile flow seaward, influencing the submarine morphology of Kilauea's south flank. Low velocities (5.0-6.3??km/s) seaward of Kilauea's Hilina fault zone, and along Mauna Loa's seaward facing Kao'iki fault zone, are attributed to thick piles of volcaniclastic sediments deposited on the submarine flanks. Loihi seamount shows high-velocity anomalies beneath the summit and along the rift zones, similar to the interpreted magma cumulates below Mauna Loa and Kilauea volcanoes, and a low-velocity anomaly beneath the oceanic crust, probably indicative of melt within the upper mantle. Around Kilauea's submarine flank, a high-velocity anomaly beneath the outer bench suggests the presence of an ancient seamount that may obstruct outward spreading of the flank. Mauna Loa's southeast flank is also marked by a large, anomalously high-velocity feature (7.0-7.4??km/s), interpreted to define an inactive, buried volcanic rift zone, which might provide a new explanation for the westward migration of Mauna Loa's current SWRZ and the growth of Kilauea's SWRZ. ?? 2007 Elsevier B.V. All rights reserved.

  5. Quantifying structural controls of rockfall activity on alpine limestone cliffs: a LiDAR-based geological approach in the Wetterstein Mountains, Bavarian Alps.

    NASA Astrophysics Data System (ADS)

    Jacobs, Benjamin; Krautblatter, Michael

    2016-04-01

    smaller than TLS effective resolution (0.01 to 0.1 m³ at 400 to 800 m range) using an inverse power law function. However, the effect of secondary and primary rockfall on the results is still subject of discussion. Also, locally unique stress fields and the history of local rock slope evolution is an issue. Here we have chosen a well-established test site to (i) systematically analyse the structural geological imprint on rockfall activity in limestone cliffs and (ii) to validate the explanatory power of the incomplete rockfall size coverage of TLS at sites with frequent small fragmental rockfall. Krautblatter, M., Moser, M., Schrott L., Wolf, J. & Morche, D., 2012. Significance of rockfall magnitude and carbonate dissolution for rock slope erosion and geomorphic work on Alpine limestone cliffs (Reintal, German Alps). In: Geomorphology, 167-168: 1-14. DOI:10.1016/j.geomorph.2012.04.007

  6. Self-assembled 3D hierarchical sheaf-like Nb3O7(OH) nanostructures with enhanced photocatalytic activity.

    PubMed

    Hu, Pei; Hou, Dongfang; Wen, Yanwei; Shan, Bin; Chen, Chaoji; Huang, Yunhui; Hu, Xianluo

    2015-02-01

    Novel three-dimensional (3D) hierarchical Nb3O7(OH) nanostructures with a sheaf-like nanoarchitecture were fabricated for the first time by a hydrothermal process. Interestingly, the nanosheafs are composed of nanorods with an average diameter of about 25 nm. The as-prepared 3D hierarchical nanostructures possess a high surface area of 77 m(2) g(-1) with pore diameters of ca. 4.2-12.5 nm. A possible growth mechanism based on the combined Ostwald ripening and self-assembly process was proposed. It is found that both the valence-band top and the conduction-band bottom consist of O 2p and Nb 4d orbitals. Importantly, the 3D hierarchical Nb3O7(OH) nanostructures exhibit enhanced photocatalytic activity for the degradation of Rhodamine B (RhB) under UV-visible light, which is attributed to the unusual hierarchical structure, high surface area, and hybridization of energy bands. PMID:25536277

  7. 3-D numerical simulation of Yb:YAG active slabs with longitudinal doping gradient for thermal load effects assessment.

    PubMed

    Ferrara, P; Ciofini, M; Esposito, L; Hostaša, J; Labate, L; Lapucci, A; Pirri, A; Toci, G; Vannini, M; Gizzi, L A

    2014-03-10

    We present a study of Yb:YAG active media slabs, based on a ceramic layered structure with different doping levels. We developed a procedure allowing 3D numerical analysis of the slab optical properties as a consequence of the thermal load induced by the pump process. The simulations are compared with a set of experimental results in order to validate the procedure. These structured ceramics appear promising in appropriate geometrical configurations, and thus are intended to be applied in the construction of High Energy Diode Pumped Solid State Laser (DPSSL) systems working in high repetition-rate pulsed regimes. PMID:24663877

  8. Synthesis and Anti-HCV Activity of 4-Methoxy-7H-Pyrrolo[2,3-d] Pyrimidine Carbocyclic Nucleosides.

    PubMed

    Thiyagarajan, Anandarajan; Toyama, Masaaki; Baba, Masanori; Sharon, Ashoke; Bal, Chandralata

    2016-06-01

    The present study includes the exploration of new possible nucleoside mimetics based on 4-methoxy-7H-pyrrolo[2,3-d]pyrimidine carbocyclic nucleosides (4a-g), which were synthesized by 10-15 synthetic steps and characterized adequately. We report the anti-HCV activities and cytotoxicities of 4a-g. Compound 4a was analyzed by single crystal X-ray diffraction which showed some puckering in the cyclopentene ring with a 2'-endo conformation and anti-base disposition (χ = -125.7°). PMID:27152477

  9. 3D-QSAR Studies on a Series of Dihydroorotate Dehydrogenase Inhibitors: Analogues of the Active Metabolite of Leflunomide

    PubMed Central

    Li, Shun-Lai; He, Mao-Yu; Du, Hong-Guang

    2011-01-01

    The active metabolite of the novel immunosuppressive agent leflunomide has been shown to inhibit the enzyme dihydroorotate dehydrogenase (DHODH). This enzyme catalyzes the fourth step in de novo pyrimidine biosynthesis. Self-organizing molecular field analysis (SOMFA), a simple three-dimensional quantitative structure-activity relationship (3D-QSAR) method is used to study the correlation between the molecular properties and the biological activities of a series of analogues of the active metabolite. The statistical results, cross-validated rCV2 (0.664) and non cross-validated r2 (0.687), show a good predictive ability. The final SOMFA model provides a better understanding of DHODH inhibitor-enzyme interactions, and may be useful for further modification and improvement of inhibitors of this important enzyme. PMID:21686163

  10. 3-d Brownian dynamics simulations of the smallest units of an active biological material

    NASA Astrophysics Data System (ADS)

    Luettmer-Strathmann, Jutta; Paudyal, Nabina; Adeli Koudehi, Maral

    Motor proteins generate stress in a cytoskeletal network by walking on one strand of the network while being attached to another one. A protein walker in contact with two elements of the network may be considered the smallest unit of an active biological material. In vitro experiments, mathematical modeling and computer simulations have provided important insights into active matter on large and on very small length and time scales. However, it is still difficult to model the effects of local environment and interactions at intermediate scales. Recently, we developed a coarse-grained, three-dimensional model for a motor protein transporting cargo by walking on a substrate. In this work, we simulate a tethered motor protein pulling a substrate with elastic response. As the walker progresses, the retarding force due to the substrate tension increases until contact fails. We present simulation results for the effect of motor-protein activity on the tension in the substrate and the effect of the retarding force on the processivity of the molecular motor.

  11. Vanadium nanobelts coated nickel foam 3D bifunctional electrode with excellent catalytic activity and stability for water electrolysis.

    PubMed

    Yu, Yu; Li, Pei; Wang, Xiaofang; Gao, Wenyu; Shen, Zongxu; Zhu, Yanan; Yang, Shuliang; Song, Weiguo; Ding, Kejian

    2016-05-19

    Pursuit of highly active, stable and low-cost electrocatalysts for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is the key point for large-scale water splitting. A vanadium nanobelts coating on a nickel foam (V/NF) is proposed as an excellent 3D bifunctional electrode for water electrolysis here, which exhibits high activities with overpotentials of 292 and 176 mV at 10 mA cm(-2) for OER and HER, respectively. When employed as a bifunctional electrocatalyst in an alkaline water electrolyzer, a cell voltage of 1.80 V was required to achieve 20 mA cm(-2) with a slight increase during a 24 h durability test. The existence of the appropriate amount of nitrogen and oxygen elements in the surface region of vanadium nanobelts is regarded to be responsible for the electrocatalytic activity. PMID:27152646

  12. 3-D Visualisation: Using Internet-based Activities to Enhance Student Understanding of 3-dimensional Spatial Relationships

    NASA Astrophysics Data System (ADS)

    Boyle, A. P.; Williams, M.; Williams, P.

    2011-12-01

    whether or not use of internet-based activities could enhance understanding of 3-D spatial relationships. A key finding was that pre-tests of students' spatial-visualisation abilities indicated student in different subject areas (e.g. geology, geophysics, marine science) had different ranges of ability, although post-test results showed no improvement resulting from the '3-D training methods' used. Evaluation of outcomes indicates that "own time" internet-based activities were valued by students as working at their own pace in their own time improved their ownership of the activity as well as their confidence, awareness, and cognitive understanding when dealing with 3-D spatial relationships. After this project ended we included internet-based activities in first year skills modules. Although many of these web-based resources and activities were of direct relevance to Year 1 geoscience students, the resources and activities may have wider application and, by helping students develop understanding of 3-D spatial relationships, be beneficial in other STEM disciplines at this level.

  13. A comparative DFT study of the catalytic activity of the 3d transition metal sulphides surfaces

    SciTech Connect

    Gomez-Balderas, R.; Oviedo-Roa, R; Martinez-Magadan, J M.; Amador, C.; Dixon, David A. )

    2002-10-10

    The catalytic activity of the first transition metal series sulphides for hydrodesulfurization (HDS) reactions exhibits a particular behaviour when analysed as a function of the metal position in the Periodic Table. This work reports a comparative study of the electronic structure of the bulk and of the (0 0 1) metal surface (assumed to be the reactive surface) for the Sc-Zn monosulphides. The systems were modeled using the NiAs prototype crystal structure for the bulk and by applying the supercell model with seven atomic layers for (0 0 1) surfaces. The electronic structure of closed-packed solids code based on the density-functional theory and adopting the muffin-tin approximation to the potential was employed in the calculations of the electronic properties. For the Co and Ni sulphides, the density of states (DOS) variations between the metal atom present in the bulk and the ones exposed at the surface show that at the surface, there exists a higher DOS in the occupied states region just below the Fermi level. This feature might indicate a good performance of these two metal sulphides substrates in the HDS reactions favouring a donation, back-donation mechanism. In contrast, the DOS at the surface of Mn is increased in the unoccupied states region, just above the Fermi level. This suggests the possibility of a strong interaction with charge dontating sulphur adsorbate atoms poisoning the active substrate surface.

  14. Vanadium nanobelts coated nickel foam 3D bifunctional electrode with excellent catalytic activity and stability for water electrolysis

    NASA Astrophysics Data System (ADS)

    Yu, Yu; Li, Pei; Wang, Xiaofang; Gao, Wenyu; Shen, Zongxu; Zhu, Yanan; Yang, Shuliang; Song, Weiguo; Ding, Kejian

    2016-05-01

    Pursuit of highly active, stable and low-cost electrocatalysts for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is the key point for large-scale water splitting. A vanadium nanobelts coating on a nickel foam (V/NF) is proposed as an excellent 3D bifunctional electrode for water electrolysis here, which exhibits high activities with overpotentials of 292 and 176 mV at 10 mA cm-2 for OER and HER, respectively. When employed as a bifunctional electrocatalyst in an alkaline water electrolyzer, a cell voltage of 1.80 V was required to achieve 20 mA cm-2 with a slight increase during a 24 h durability test. The existence of the appropriate amount of nitrogen and oxygen elements in the surface region of vanadium nanobelts is regarded to be responsible for the electrocatalytic activity.Pursuit of highly active, stable and low-cost electrocatalysts for hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) is the key point for large-scale water splitting. A vanadium nanobelts coating on a nickel foam (V/NF) is proposed as an excellent 3D bifunctional electrode for water electrolysis here, which exhibits high activities with overpotentials of 292 and 176 mV at 10 mA cm-2 for OER and HER, respectively. When employed as a bifunctional electrocatalyst in an alkaline water electrolyzer, a cell voltage of 1.80 V was required to achieve 20 mA cm-2 with a slight increase during a 24 h durability test. The existence of the appropriate amount of nitrogen and oxygen elements in the surface region of vanadium nanobelts is regarded to be responsible for the electrocatalytic activity. Electronic supplementary information (ESI) available: More SEM, TEM images, XRD patterns, LSV curves, XPS spectra. See DOI: 10.1039/c6nr02395a

  15. Fabrication of a 3D active mixer based on deformable Fe-doped PDMS cones with magnetic actuation

    NASA Astrophysics Data System (ADS)

    Riahi, Mohammadreza; Alizadeh, Elaheh

    2012-11-01

    In this paper an active 3D mixer for lab-on-chip applications is presented. The micrometer size cone shape holes are ablated on a PMMA sheet utilizing a CO2 laser. The holes are filled with Fe micro-particles and the whole structure is molded with PDMS which cause the Fe micro-particles to be trapped in a PDMS cone structure. These Fe-doped PDMS cones are placed in a PMMA micro-channel structure fabricated by CO2 laser machining. By applying an external periodic magnetic field, the cones periodically bend in the micro-channel and stir the fluid. The fabrication method and the effect of the magnetic field on the bending of the cones with different aspect ratios is also discussed utilizing computer simulation. Doping the polymers with micro- and nano-metallic particles has been carried out by different research groups before, but according to our knowledge, application of such structures for the fabrication of a 3D active mixer has not been presented before.

  16. Synthesis, characterization and antifungal activities of 3d-transition metal complexes of 1-acetylpiperazinyldithioc arbamate, M(acpdtc) 2

    NASA Astrophysics Data System (ADS)

    Mohammad, Ali; Varshney, Charu; Nami, Shahab A. A.

    2009-07-01

    A series of mononuclear 3d-transition metal complexes of the type M(acpdtc) 2 have been synthesized (where acpdtc = 1-acetylpiperazinyldithiocarbamate, M = Mn(II), Fe(II), Co(II), Ni(II) and Cu(II)). The ligand and its complexes have been characterized by micro analysis (CHNS), TG/DSC, FT-IR, UV-vis, 1H NMR, magnetic susceptibility and conductance measurements. On the basis IR spectroscopy a symmetrical bidentate coordination has been observed for the 1-acetylpiperazinyldithiocarbamate moiety in all the complexes. On the basis of UV-vis spectra and magnetic susceptibility measurement a square-planar geometry has been proposed for the Ni(II) and Cu(II) complexes while the other complexes have been found to acquire a distorted-tetrahedral structure. The thermogravimetric and differential scanning calorimetric profile of the ligand indicates a two-step decomposition pattern while the complexes exhibit a three-stage thermogram forming metal sulfide as the eventual end product. The molar conductivity data of 1 mM solution in DMSO of the complexes is in close accord to their non-electrolytic behaviour. The ligand and its 3d-transition metal complexes have also been tested for their antifungicidal activity by agar well diffusion method using Fusarium sp. and Sclerotina sp. The maximum activity has been observed in case of Mn(II) and Fe(II) complexes.

  17. A 3D transport model study of chlorine activation during EASOE

    SciTech Connect

    Chipperfield, M.P.; Cariolle, D.; Simon, P. )

    1994-06-22

    The authors present the results of a chemical transport model which was applied to the problem of atmospheric chemistry of trace species in the stratosphere during EASOE. The model uses a comprehensive set of gas phase reactions, as well as modeling reactions occuring on nuclei in polar stratospheric clouds. This model has been used to study the development of chlorine compounds during the winter of 1991-92. Meteorological data is used as a boundary condition for the model. Significant increases in reactive chlorine are shown to occur in the lower stratosphere through mid January, when warming reduced the occurrence of polar stratospheric clouds. Even with the enhanced active chlorine presence, the lack of sunlight inhibited substantial chemical destruction of ozone.

  18. An active system for visually-guided reaching in 3D across binocular fixations.

    PubMed

    Martinez-Martin, Ester; del Pobil, Angel P; Chessa, Manuela; Solari, Fabio; Sabatini, Silvio P

    2014-01-01

    Based on the importance of relative disparity between objects for accurate hand-eye coordination, this paper presents a biological approach inspired by the cortical neural architecture. So, the motor information is coded in egocentric coordinates obtained from the allocentric representation of the space (in terms of disparity) generated from the egocentric representation of the visual information (image coordinates). In that way, the different aspects of the visuomotor coordination are integrated: an active vision system, composed of two vergent cameras; a module for the 2D binocular disparity estimation based on a local estimation of phase differences performed through a bank of Gabor filters; and a robotic actuator to perform the corresponding tasks (visually-guided reaching). The approach's performance is evaluated through experiments on both simulated and real data. PMID:24672295

  19. An Active System for Visually-Guided Reaching in 3D across Binocular Fixations

    PubMed Central

    2014-01-01

    Based on the importance of relative disparity between objects for accurate hand-eye coordination, this paper presents a biological approach inspired by the cortical neural architecture. So, the motor information is coded in egocentric coordinates obtained from the allocentric representation of the space (in terms of disparity) generated from the egocentric representation of the visual information (image coordinates). In that way, the different aspects of the visuomotor coordination are integrated: an active vision system, composed of two vergent cameras; a module for the 2D binocular disparity estimation based on a local estimation of phase differences performed through a bank of Gabor filters; and a robotic actuator to perform the corresponding tasks (visually-guided reaching). The approach's performance is evaluated through experiments on both simulated and real data. PMID:24672295

  20. Therapeutic Strategy for the Prevention of Pseudorabies Virus Infection in C57BL/6 Mice by 3D8 scFv with Intrinsic Nuclease Activity

    PubMed Central

    Lee, Gunsup; Cho, SeungChan; Hoang, Phuong Mai; Kim, Dongjun; Lee, Yongjun; Kil, Eui-Joon; Byun, Sung-June; Lee, Taek-Kyun; Kim, Dae-Hyun; Kim, Sunghan; Lee, Sukchan

    2015-01-01

    3D8 single chain variable fragment (scFv) is a recombinant monoclonal antibody with nuclease activity that was originally isolated from autoimmune-prone MRL mice. In a previous study, we analyzed the nuclease activity of 3D8 scFv and determined that a HeLa cell line expressing 3D8 scFv conferred resistance to herpes simplex virus type 1 (HSV-1) and pseudorabies virus (PRV). In this study, we demonstrate that 3D8 scFv could be delivered to target tissues and cells where it exerted a therapeutic effect against PRV. PRV was inoculated via intramuscular injection, and 3D8 scFv was injected intraperitoneally. The observed therapeutic effect of 3D8 scFv against PRV was also supported by results from quantitative reverse transcription polymerase chain reaction, southern hybridization, and immunohistochemical assays. Intraperitoneal injection of 5 and 10 μg 3D8 scFv resulted in no detectable toxicity. The survival rate in C57BL/6 mice was 9% after intramuscular injection of 10 LD50 PRV. In contrast, the 3D8 scFv-injected C57BL/6 mice showed survival rates of 57% (5 μg) and 47% (10 μg). The results indicate that 3D8 scFv could be utilized as an effective antiviral agent in several animal models. PMID:26255831

  1. Holocene - historical - decadal - annual retreat rates along the eastern Mediterranean coastal cliff of Israel

    NASA Astrophysics Data System (ADS)

    Katz, Oded; Mushkin, Amit; Kober, Florian; Porat, Naomi

    2013-04-01

    The Israeli coastal cliff extends about 50 km along the eastern Mediterranean and is comprised of late Quaternary eolianites and paleosols that reach heights of up to 50 meters above current sea-level. Variable cliff-top inland retreat rates of up to a few tens of centimeters per year have been previously measured along the cliff line usually by comparing aerial photos from the last decades. Commonly, these locally constrained retreat rates have been: 1) extrapolated as representative of the entire cliff length, and 2) adopted by hazard-mitigation and planning authorities. Here, we re-evaluate the current understanding of retreat patterns and rates along this cliff line using a suite of ground-based LiDAR observations, airborne LiDAR, aerial photography, archeology and numerical (OSL) age determination of older coastal cliff locations. Annual retreat rates and patterns were constrained using repeat high-resolution ground based LiDAR. In places, storm induced landward cliff retreat reached 7 m/yr, which is comparable to the total retreat previously documented along this coastal stretch during the past 60 years. Yet, spatially - cliff-retreat activity per given year was documented along less than 5% of the entire cliff length. Decadal-scale retreat rates along the entire coastal cliff were mapped by comparing the cliff-top location in aerial photos from 1945 and 2004. These revealed cliff-top retreat rates of less than 0.1 m/yr along 58 % of the cliff length and >0.4 m/yr retreat rates along only several % of the cliff length. Extended stretches of the cliff line (~50%) did not experience detectable retreat in the past 60 years and thus appear to have remained stable during this time period. Local retreat rates at centennial to millennial time scales were constrained at a cliff-top archeological site where a Crusader castle appears to have lost <20 m of its outer walls as well as at a natural site where the past locations of the cliff are marked by multiple cliff

  2. Comparison of Different 2D and 3D-QSAR Methods on Activity Prediction of Histamine H3 Receptor Antagonists.

    PubMed

    Dastmalchi, Siavoush; Hamzeh-Mivehroud, Maryam; Asadpour-Zeynali, Karim

    2012-01-01

    Histamine H3 receptor subtype has been the target of several recent drug development programs. Quantitative structure-activity relationship (QSAR) methods are used to predict the pharmaceutically relevant properties of drug candidates whenever it is applicable. The aim of this study was to compare the predictive powers of three different QSAR techniques, namely, multiple linear regression (MLR), artificial neural network (ANN), and HASL as a 3D QSAR method, in predicting the receptor binding affinities of arylbenzofuran histamine H3 receptor antagonists. Genetic algorithm coupled partial least square as well as stepwise multiple regression methods were used to select a number of calculated molecular descriptors to be used in MLR and ANN-based QSAR studies. Using the leave-group-out cross-validation technique, the performances of the MLR and ANN methods were evaluated. The calculated values for the mean absolute percentage error (MAPE), ranging from 2.9 to 3.6, and standard deviation of error of prediction (SDEP), ranging from 0.31 to 0.36, for both MLR and ANN methods were statistically comparable, indicating that both methods perform equally well in predicting the binding affinities of the studied compounds toward the H3 receptors. On the other hand, the results from 3D-QSAR studies using HASL method were not as good as those obtained by 2D methods. It can be concluded that simple traditional approaches such as MLR method can be as reliable as those of more advanced and sophisticated methods like ANN and 3D-QSAR analyses. PMID:25317190

  3. MODELING STATISTICAL PROPERTIES OF SOLAR ACTIVE REGIONS THROUGH DIRECT NUMERICAL SIMULATIONS OF 3D-MHD TURBULENCE

    SciTech Connect

    Malapaka, Shiva Kumar; Mueller, Wolf-Christian

    2013-09-01

    Statistical properties of the Sun's photospheric turbulent magnetic field, especially those of the active regions (ARs), have been studied using the line-of-sight data from magnetograms taken by the Solar and Heliospheric Observatory and several other instruments. This includes structure functions and their exponents, flatness curves, and correlation functions. In these works, the dependence of structure function exponents ({zeta}{sub p}) of the order of the structure functions (p) was modeled using a non-intermittent K41 model. It is now well known that the ARs are highly turbulent and are associated with strong intermittent events. In this paper, we compare some of the observations from Abramenko et al. with the log-Poisson model used for modeling intermittent MHD turbulent flows. Next, we analyze the structure function data obtained from the direct numerical simulations (DNS) of homogeneous, incompressible 3D-MHD turbulence in three cases: sustained by forcing, freely decaying, and a flow initially driven and later allowed to decay (case 3). The respective DNS replicate the properties seen in the plots of {zeta}{sub p} against p of ARs. We also reproduce the trends and changes observed in intermittency in flatness and correlation functions of ARs. It is suggested from this analysis that an AR in the onset phase of a flare can be treated as a forced 3D-MHD turbulent system in its simplest form and that the flaring stage is representative of decaying 3D-MHD turbulence. It is also inferred that significant changes in intermittency from the initial onset phase of a flare to its final peak flaring phase are related to the time taken by the system to reach the initial onset phase.

  4. Visible-Light-Induced Olefin Activation Using 3D Aromatic Boron-Rich Cluster Photooxidants.

    PubMed

    Messina, Marco S; Axtell, Jonathan C; Wang, Yiqun; Chong, Paul; Wixtrom, Alex I; Kirlikovali, Kent O; Upton, Brianna M; Hunter, Bryan M; Shafaat, Oliver S; Khan, Saeed I; Winkler, Jay R; Gray, Harry B; Alexandrova, Anastassia N; Maynard, Heather D; Spokoyny, Alexander M

    2016-06-01

    We report a discovery that perfunctionalized icosahedral dodecaborate clusters of the type B12(OCH2Ar)12 (Ar = Ph or C6F5) can undergo photo-excitation with visible light, leading to a new class of metal-free photooxidants. Excitation in these species occurs as a result of the charge transfer between low-lying orbitals located on the benzyl substituents and an unoccupied orbital delocalized throughout the boron cluster core. Here we show how these species, photo-excited with a benchtop blue LED source, can exhibit excited-state reduction potentials as high as 3 V and can participate in electron-transfer processes with a broad range of styrene monomers, initiating their polymerization. Initiation is observed in cases of both electron-rich and electron-deficient styrene monomers at cluster loadings as low as 0.005 mol%. Furthermore, photo-excitation of B12(OCH2C6F5)12 in the presence of a less activated olefin such as isobutylene results in the production of highly branched poly(isobutylene). This work introduces a new class of air-stable, metal-free photo-redox reagents capable of mediating chemical transformations. PMID:27186856

  5. Wool Keratin 3D Scaffolds with Light-Triggered Antimicrobial Activity.

    PubMed

    Ferroni, Claudia; Sotgiu, Giovanna; Sagnella, Anna; Varchi, Greta; Guerrini, Andrea; Giuri, Demetra; Polo, Eleonora; Orlandi, Viviana Teresa; Marras, Emanuela; Gariboldi, Marzia; Monti, Elena; Aluigi, Annalisa

    2016-09-12

    Photoactivatable keratin sponges were prepared from protein aqueous solutions by the freeze-drying method, followed by photofunctionalization with two different photosensitizers (PS): Azure A (AzA) and 5,10,15,20-tetrakis [4-(2-N,N,N-trimethylethylthio)-2,3,5,6-tetrafluorophenyl]porphyrin tetraiodide salt (TTFAP). The prepared sponges have a porosity between 49% and 80% and a mean pore size in the 37-80 μm range. As compared to AzA, TTFAP interacts more strongly with the sponges as demonstrated by a lower PS release (6% vs 20%), a decreased swelling ratio (1.6 vs 7.4), and a slower biodegradation rate. Nevertheless, AzA-loaded sponges showed the highest photoactivity, as also demonstrated by their higher antibactericidal activity toward both Gram-positive and Gram-negative bacteria. The obtained results suggest that the antimicrobial photodynamic effect can be finely triggered through a proper selection of the amount and type of photosensitizer, as well as through the irradiation time. Finally, all the prepared sponges support human fibroblast cells growth, while no significant cell viability impairment is observed upon light irradiation. PMID:27463471

  6. Synthesis and Biological Activity of New Thiopyrano[2,3-d]thiazoles Containing a Naphthoquinone Moiety

    PubMed Central

    Atamanyuk, Dmytro; Zimenkovsky, Borys; Atamanyuk, Vasyl; Nektegayev, Ihor; Lesyk, Roman

    2013-01-01

    Novel 11-substituted 3,11-dihydro-2H-benzo[6,7]thiochromeno[2,3-d][1,3]-thiazole-2,5,10-triones 4a–i were synthesized in 75–90% yields via the hetero-Diels-Alder reaction of 5-arylidene-4-thioxo-2-thiazolidinones with 1,4-naphthoquinone. The synthesized compounds were evaluated for their antineoplastic and antimycobacterial activities. A moderate selectivity against melanoma cancer cells (GI50 (UACC-257-melanoma) = 0.22 μM) was demonstrated for 4i, whereas derivatives 4a, 4c, 4g, and 4h showed promising antimycobacterial activity at a low toxicity level. PMID:23833711

  7. Prostate boundary segmentation from ultrasound images using 2D active shape models: optimisation and extension to 3D.

    PubMed

    Hodge, Adam C; Fenster, Aaron; Downey, Dónal B; Ladak, Hanif M

    2006-12-01

    Boundary outlining, or segmentation, of the prostate is an important task in diagnosis and treatment planning for prostate cancer. This paper describes an algorithm based on two-dimensional (2D) active shape models (ASM) for semi-automatic segmentation of the prostate boundary from ultrasound images. Optimisation of the 2D ASM for prostatic ultrasound was done first by examining ASM construction and image search parameters. Extension of the algorithm to three-dimensional (3D) segmentation was then done using rotational-based slicing. Evaluation of the 3D segmentation algorithm used distance- and volume-based error metrics to compare algorithm generated boundary outlines to gold standard (manually generated) boundary outlines. Minimum description length landmark placement for ASM construction, and specific values for constraints and image search were found to be optimal. Evaluation of the algorithm versus gold standard boundaries found an average mean absolute distance of 1.09+/-0.49 mm, an average percent absolute volume difference of 3.28+/-3.16%, and a 5x speed increase versus manual segmentation. PMID:16930764

  8. Multifunctional bioscaffolds for 3D culture of melanoma cells reveal increased MMP activity and migration with BRAF kinase inhibition.

    PubMed

    Leight, Jennifer L; Tokuda, Emi Y; Jones, Caitlin E; Lin, Austin J; Anseth, Kristi S

    2015-04-28

    Matrix metalloproteinases (MMPs) are important for many different types of cancer-related processes, including metastasis. Understanding the functional impact of changes in MMP activity during cancer treatment is an important facet not typically evaluated as part of preclinical research. With MMP activity being a critical component of the metastatic cascade, we designed a 3D hydrogel system to probe whether pharmacological inhibition affected human melanoma cell proteolytic activity; metastatic melanoma is a highly aggressive and drug-resistant form of skin cancer. The relationship between MMP activity and drug treatment is unknown, and therefore we used an in situ fluorogenic MMP sensor peptide to determine how drug treatment affects melanoma cell MMP activity in three dimensions. We encapsulated melanoma cells from varying stages of progression within PEG-based hydrogels to examine the relationship between drug treatment and MMP activity. From these results, a metastatic melanoma cell line (A375) and two inhibitors that inhibit RAF (PLX4032 and sorafenib) were studied further to determine whether changes in MMP activity led to a functional change in cell behavior. A375 cells exhibited increased MMP activity despite an overall decrease in metabolic activity with PLX4032 treatment. The changes in proteolytic activity correlated with increased cell elongation and increased single-cell migration. In contrast, sorafenib did not alter MMP activity or cell motility, showing that the changes induced by PLX4032 were not a universal response to small-molecule inhibition. Therefore, we argue the importance of studying MMP activity with drug treatment and its possible implications for unwanted side effects. PMID:25870264

  9. Stability of permafrost dominated coastal cliffs in the Arctic

    NASA Astrophysics Data System (ADS)

    Hoque, Md. Azharul; Pollard, Wayne H.

    2016-03-01

    Block failure is considered to be an important component of coastal retreat in permafrost regions. A comprehensive model is developed to study the effects of thermoerosional niche and ice wedge morphology on the stability of permafrost dominated coastal cliff against block failure. The model is formulated by coupling slope stability analysis with a time dependent progression of thermoerosional niches and the morphology of the nearby ice wedges. Model computations are initially performed for failure conditions for a given cliff height, frozen soil strength, ice content, water pressure in the active layer, thermoerosional niche depth and ice wedge morphology. Under these conditions block failures are found to be predominantly overturning failures and are governed by the tensile strength of frozen soil, thermoerosional niche depth and ice wedge location and depth. The effects of ice wedges are then examined by analyzing failure conditions for ice wedges of different locations and depths. For a given cliff height, strength and thermoerosional niche, block failure may occur at a range of different combinations of ice wedge locations and depths. Two stability nomograms are developed through repeated model calculations for range of cliff heights and frozen soil tensile strength. These nomograms can be used to determine the critical combinations of thermoerosional niche depth, ice wedge distance and ice wedge depth that lead to block collapse of a cliff of known height and soil strength. Some analytical expressions are also derived to determine potential block failure criteria along Arctic coasts.

  10. Pentafluorophenylammonium triflate (PFPAT) catalyzed facile construction of substituted chromeno[2,3-d]pyrimidinone derivatives and their antimicrobial activity

    PubMed Central

    Ghashang, Majid; Mansoor, Syed Sheik; Aswin, Krishnamoorthy

    2013-01-01

    A new, simple thermally efficient and solvent-free condensation of 2-amino-3-cyano-6-methyl-4-phenyl-4H-pyran-5-ethylcarboxylate derivatives with coumarin-3-carboxylic acid employing pentafluorophenylammonium triflate (PFPAT) as an inexpensive organocatalyst for the synthesis of a series of ethyl 4,5-dihydro 7-methyl-2-(2-oxo-2H-chromen-3-yl)-4-oxo-5-aryl-3H-chromeno[2,3-d]pyrimidine-6-carboxylate derivatives is described. This method has the advantages of high yields, a cleaner reaction, simple methodology, short reaction times, easy workup, and greener conditions. All the compounds were evaluated for their in vitro antimicrobial activity against different bacterial and fungal strains. PMID:25685489

  11. Molecular docking and 3D-QSAR studies on the glucocorticoid receptor antagonistic activity of hydroxylated polychlorinated biphenyls.

    PubMed

    Liu, S; Luo, Y; Fu, J; Zhou, J; Kyzas, G Z

    2016-02-01

    The glucocorticoid receptor (GR) antagonistic activities of hydroxylated polychlorinated biphenyls (HO-PCBs) were recently characterised. To further explore the interactions between HO-PCBs and the GR, and to elucidate structural characteristics that influence the GR antagonistic activity of HO-PCBs, molecular docking and three-dimensional quantitative structure-activity relationship (3D-QSAR) studies were performed. Comparative molecular similarity indices analysis (CoMSIA) was performed using both ligand- and receptor-based alignment schemes. Results generated from the receptor-based model were found to be more satisfactory, with q(2) of 0.632 and r(2) of 0.931 compared with those from the ligand-based model. Some internal validation strategies (e.g. cross-validation analysis, bootstrapping analysis and Y-randomisation) and an external validation method were used respectively to further assess the stability and predictive ability of the derived model. Graphical interpretation of the model provided some insights into the structural features that affected the GR antagonistic activity of HO-PCBs. Molecular docking studies revealed that some key residues were critical for ligand-receptor interactions by forming hydrogen bonds (Glu540) and hydrophobic interactions with ligands (Ile539, Val543 and Trp577). Although CoMSIA sometimes depends on the alignment of the molecules, the information provided is beneficial for predicting the GR antagonistic activities of HO-PCB homologues and is helpful for understanding the binding mechanisms of HO-PCBs to GR. PMID:26848875

  12. 3D modeling, ligand binding and activation studies of the cloned mouse delta, mu; and kappa opioid receptors.

    PubMed

    Filizola, M; Laakkonen, L; Loew, G H

    1999-11-01

    Refined 3D models of the transmembrane domains of the cloned delta, mu and kappa opioid receptors belonging to the superfamily of G-protein coupled receptors (GPCRs) were constructed from a multiple sequence alignment using the alpha carbon template of rhodopsin recently reported. Other key steps in the procedure were relaxation of the 3D helix bundle by unconstrained energy optimization and assessment of the stability of the structure by performing unconstrained molecular dynamics simulations of the energy optimized structure. The results were stable ligand-free models of the TM domains of the three opioid receptors. The ligand-free delta receptor was then used to develop a systematic and reliable procedure to identify and assess putative binding sites that would be suitable for similar investigation of the other two receptors and GPCRs in general. To this end, a non-selective, 'universal' antagonist, naltrexone, and agonist, etorphine, were used as probes. These ligands were first docked in all sites of the model delta opioid receptor which were sterically accessible and to which the protonated amine of the ligands could be anchored to a complementary proton-accepting residue. Using these criteria, nine ligand-receptor complexes with different binding pockets were identified and refined by energy minimization. The properties of all these possible ligand-substrate complexes were then examined for consistency with known experimental results of mutations in both opioid and other GPCRs. Using this procedure, the lowest energy agonist-receptor and antagonist-receptor complexes consistent with these experimental results were identified. These complexes were then used to probe the mechanism of receptor activation by identifying differences in receptor conformation between the agonist and the antagonist complex during unconstrained dynamics simulation. The results lent support to a possible activation mechanism of the mouse delta opioid receptor similar to that recently

  13. Active site characterization and structure based 3D-QSAR studies on non-redox type 5-lipoxygenase inhibitors.

    PubMed

    Ul-Haq, Zaheer; Khan, Naveed; Zafar, Syed Kashif; Moin, Syed Tarique

    2016-06-10

    Structure-based 3D-QSAR study was performed on a class of 5-benzylidene-2-phenylthiazolinones non-redox type 5-LOX inhibitors. In this study, binding pocket of 5-Lipoxygenase (pdb id 3o8y) was identified by manual docking using 15-LOX (pdb id 2p0m) as a reference structure. Additionally, most of the binding site residues were found conserved in both structures. These non-redox inhibitors were then docked into the binding site of 5-LOX. To generate reliable CoMFA and CoMSIA models, atom fit data base alignment method using docked conformation of the most active compound was employed. The q(2)cv and r(2)ncv values for CoMFA model were found to be 0.549 and 0.702, respectively. The q(2)cv and r(2)ncv values for the selected CoMSIA model comprised four descriptors steric, electrostatic, hydrophobic and hydrogen bond donor fields were found to be 0.535 and 0.951, respectively. Obtained results showed that our generated model was statistically reliable. Furthermore, an external test set validates the reliability of the predicted model by calculating r(2)pred i.e.0.787 and 0.571 for CoMFA and CoMSIA model, respectively. 3D contour maps generated from CoMFA and CoMSIA models were utilized to determine the key structural features of ligands responsible for biological activities. The applied protocol will be helpful to design more potent and selective inhibitors of 5-LOX. PMID:27044904

  14. The DOSIS and DOSIS 3D Experiments onboard the International Space Station - Results from the Active DOSTEL Instruments

    NASA Astrophysics Data System (ADS)

    Burmeister, Soenke; Berger, Thomas; Reitz, Guenther; Beaujean, Rudolf; Boehme, Matthias; Haumann, Lutz; Labrenz, Johannes; Kortmann, Onno

    2012-07-01

    Besides the effects of the microgravity environment, and the psychological and psychosocial problems experienced in confined spaces, radiation is the main health detriment for long duration human space missions. The radiation environment encountered in space differs in nature from that on earth, consisting mostly of high energetic ions from protons up to iron, resulting in radiation levels far exceeding the ones present on earth for occupational radiation workers. Accurate knowledge of the physical characteristics of the space radiation field in dependence on the solar activity, the orbital parameters and the different shielding configurations of the International Space Station ISS is therefore needed. For the investigation of the spatial and temporal distribution of the radiation field inside the European COLUMBUS module the experiment DOSIS (Dose Distribution Inside the ISS) under the lead of DLR was launched on July 15th 2009 with STS-127 to the ISS. The experimental package was transferred from the Space Shuttle into COLUMBUS on July 18th. It consists of a combination of passive detector packages (PDP) distributed at 11 locations inside the European Columbus Laboratory and two active radiation detectors (DOSTELs) with a DDPU (DOSTEL Data and Power Unit) in a nomex pouch (DOSIS MAIN BOX) mounted at a fixed location beneath the European Physiology Module rack (EPM) inside COLUMBUS. The DOSTELs measured during the lowest solar minimum conditions in the space age from July 18th 2009 to June 16th 2011. In July 2011 the active hardware was transferred to ground for refurbishment and preparation for the DOSIS-3D experiment. The hardware will be launched with the Soyuz 30S flight to the ISS on May 15th 2012 and activated approximately ten days later. Data will be transferred from the DOSTEL units to ground via the EPM rack which is activated approximately every four weeks for this action. First Results for the active DOSIS-3D measurements such as count rate profiles

  15. SU-E-T-230: Measurement of Proton-Activated Positron Emission with PRESAGE 3-D Dosimeters

    SciTech Connect

    Carroll, M; Mawlawi, O; Ibbott, G; Adamovics, J

    2014-06-01

    Purpose: Measurement of positron emission following proton beam irradiation of a target has been studied as a method of in-vivo dosimetry. Relative dosimetry studies between a phantom and treatment plan are susceptible to range uncertainties from material heterogeneities and setup error. By using the radiochromic polyurethane dosimeter PRESAGE, we can correlate the proton dose distribution to the PET activity measurement within a single detector. The PRESAGE formulation used was developed for high-LET proton radiotherapy, has similar density and RLSP to tissue, and consists of a greater carbon component, which gives it a higher positron signal than many other 3D detectors. Methods: Three cylindrical PRESAGE dosimeters were irradiated semi-uniformly to 500 cGy with 180- MeV protons. The beam was directed along the dosimeter axis and delivered a 2-cm SOBP at the center of the dosimeter. The dosimeters were rushed to a nearby PET/CT where imaging began within 15 minutes, less than a single half-life of 11C. A 3-hr measurement captured the full activation decay. Afterwards, the dose profiles were measured by optical-CT. A direct comparison between the measured dose and the positron emission was performed using CERR software. Results: The correlations between dose distributions and PET activity were consistent with previous studies in that the proximal region of the SOBP displayed the highest activity. The spatial distributions between the dose and activity were similar. Along the central axis of the beam, we found a shift in the distal 80% of 1 cm. The lateral profile showed good agreement between dose and activity. PET imaging times between 30-min and 3-hrs showed <5% discrepancy. Conclusion: PRESAGE dosimeters offer a strong and unique potential to accurately correlate dosimetric and PET activation information. Implementation in an anthropomorphic phantom could be used to study representative treatment plans. NIH grant 5R01CA100835.

  16. CliffsQuickReview Physics

    NASA Astrophysics Data System (ADS)

    Huetinck, Linda; Ph., D.; Adams, Scott

    2001-06-01

    CliffsQuickReview course guides cover the essentials of your toughest subjects. Get a firm grip on core concepts and key material, and test your newfound knowledge with review questions. Whether you need a course supplement, help preparing for a physics exam, or a concise reference for physics, CliffsQuickReview Physics can help. This guide provides a valuable introduction to the concepts of classical mechanics, thermodynamics, magnetism, and electricity. In no time, you'll be ready to tackle other concepts in this book such as Wave motion and sound Current and resistance Electromagnetic induction Geometrical optics Nuclear physics Quantum mechanics CliffsQuickReview Physics acts as a supplement to your other learning material. Use this reference in any way that fits your personal style for study and review -- you decide what works best with your needs. You can flip through the book until you find what you're looking for -- it's organized to gradually build on key concepts. Or, here are just a few other ways you can search for topics: Use the free Pocket Guide full of essential information Get a glimpse of what you'll gain from a chapter by reading through the Chapter Check-In at the beginning of each chapter Use the Chapter Checkout at the end of each chapter to gauge your grasp of the important information you need to know Test your knowledge more completely in the CQR Review and look for additional sources of information in the CQR Resource Center Use the glossary to find key terms fast. With titles available for all the most popular high school and college courses, CliffsQuickReview guides are a comprehensive resource that can help you get the best possible grades.

  17. California Sea Cliff Metrics: Mapping and Validation

    NASA Astrophysics Data System (ADS)

    Palaseanu, M.; Thatcher, C.; Danielson, J.; Logan, J. B.; Foxgrover, A. C.; Brock, J. C.; Barnard, P.

    2015-12-01

    Seacliff erosion is a serious hazard with implications for coastal management, and is often estimated using successive hand digitized cliff tops or bases (toe) to assess cliff retreat. We developed an automated procedure to extract the location of the cliff top from high resolution lidar-derived digital elevation models using transects generated at approximately 1-m intervals. The automated method to define cliff tops is repeatable, takes advantage of detailed topographic information within high-resolution elevation data, and is more efficient than hand-digitizing. To validate the results obtained from a 2010 aerial lidar survey, we conducted a terrestrial lidar (tlidar) survey at Bonny Doon beach near Santa Cruz California in 2014 and mapped the location of the cliff tops using real-time kinematic GPS. Bonny Doon beach has highly irregular cliffs, with several small sea caves and erosion features that were not distinguishable in the aerial lidar digital terrain model (DTM). We extracted the location of the cliff top from the tlidar point cloud along the same transects used previously to automatically derive cliff tops from the aerial lidar derived DTM. The minimum horizontal distance between the tlidar derived cliff top points and GPS points was calculated. The error measurements between GPS and terrestrial lidar are 0.19 m mean absolute error (MAE) and 0.51 m root mean square error (RMSE) respectively. The MAE and the RMSE between the GPS and aerial lidar cliff top points were 0.96 and 1.82 m respectively. The larger errors between aerial lidar cliff top points and GPS are likely due to the failure to remove all vegetation from the aerial lidar data, the positional accuracy of the aerial lidar, and a 4-year gap between surveys. The error assessment indicates that the automated procedure mapped the cliff top location successfully using both the aerial and terrestrial lidar, although the cliff top delineation based on the tlidar data was more accurate.

  18. Short and long time effects of low temperature Plasma Activated Media on 3D multicellular tumor spheroids

    PubMed Central

    Judée, Florian; Fongia, Céline; Ducommun, Bernard; Yousfi, Mohammed; Lobjois, Valérie; Merbahi, Nofel

    2016-01-01

    This work investigates the regionalized antiproliferative effects of plasma-activated medium (PAM) on colon adenocarcinoma multicellular tumor spheroid (MCTS), a model that mimics 3D organization and regionalization of a microtumor region. PAM was generated by dielectric barrier plasma jet setup crossed by helium carrier gas. MCTS were transferred in PAM at various times after plasma exposure up to 48 hours and effect on MCTS growth and DNA damage were evaluated. We report the impact of plasma exposure duration and delay before transfer on MCTS growth and DNA damage. Local accumulation of DNA damage revealed by histone H2AX phosphorylation is observed on outermost layers and is dependent on plasma exposure. DNA damage is completely reverted by catalase addition indicating that H2O2 plays major role in observed genotoxic effect while growth inhibitory effect is maintained suggesting that it is due to others reactive species. SOD and D-mannitol scavengers also reduced DNA damage by 30% indicating that and OH* are involved in H2O2 formation. Finally, PAM is able to retain its cytotoxic and genotoxic activity upon storage at +4 °C or −80 °C. These results suggest that plasma activated media may be a promising new antitumor strategy for colorectal cancer tumors. PMID:26898904

  19. Short and long time effects of low temperature Plasma Activated Media on 3D multicellular tumor spheroids

    NASA Astrophysics Data System (ADS)

    Judée, Florian; Fongia, Céline; Ducommun, Bernard; Yousfi, Mohammed; Lobjois, Valérie; Merbahi, Nofel

    2016-02-01

    This work investigates the regionalized antiproliferative effects of plasma-activated medium (PAM) on colon adenocarcinoma multicellular tumor spheroid (MCTS), a model that mimics 3D organization and regionalization of a microtumor region. PAM was generated by dielectric barrier plasma jet setup crossed by helium carrier gas. MCTS were transferred in PAM at various times after plasma exposure up to 48 hours and effect on MCTS growth and DNA damage were evaluated. We report the impact of plasma exposure duration and delay before transfer on MCTS growth and DNA damage. Local accumulation of DNA damage revealed by histone H2AX phosphorylation is observed on outermost layers and is dependent on plasma exposure. DNA damage is completely reverted by catalase addition indicating that H2O2 plays major role in observed genotoxic effect while growth inhibitory effect is maintained suggesting that it is due to others reactive species. SOD and D-mannitol scavengers also reduced DNA damage by 30% indicating that and OH* are involved in H2O2 formation. Finally, PAM is able to retain its cytotoxic and genotoxic activity upon storage at +4 °C or -80 °C. These results suggest that plasma activated media may be a promising new antitumor strategy for colorectal cancer tumors.

  20. Simultaneous spatiotemporal mapping of in situ pH and bacterial activity within an intact 3D microcolony structure.

    PubMed

    Hwang, Geelsu; Liu, Yuan; Kim, Dongyeop; Sun, Victor; Aviles-Reyes, Alejandro; Kajfasz, Jessica K; Lemos, Jose A; Koo, Hyun

    2016-01-01

    Biofilms are comprised of bacterial-clusters (microcolonies) enmeshed in an extracellular matrix. Streptococcus mutans can produce exopolysaccharides (EPS)-matrix and assemble microcolonies with acidic microenvironments that can cause tooth-decay despite the surrounding neutral-pH found in oral cavity. How the matrix influences the pH and bacterial activity locally remains unclear. Here, we simultaneously analyzed in situ pH and gene expression within intact biofilms and measured the impact of damage to the surrounding EPS-matrix. The spatiotemporal changes of these properties were characterized at a single-microcolony level following incubation in neutral-pH buffer. The middle and bottom-regions as well as inner-section within the microcolony 3D structure were resistant to neutralization (vs. upper and peripheral-region), forming an acidic core. Concomitantly, we used a green fluorescent protein (GFP) reporter to monitor expression of the pH-responsive atpB (PatpB::gfp) by S. mutans within microcolonies. The atpB expression was induced in the acidic core, but sharply decreased at peripheral/upper microcolony regions, congruent with local pH microenvironment. Enzymatic digestion of the surrounding matrix resulted in nearly complete neutralization of microcolony interior and down-regulation of atpB. Altogether, our data reveal that biofilm matrix facilitates formation of an acidic core within microcolonies which in turn activates S. mutans acid-stress response, mediating both the local environment and bacterial activity in situ. PMID:27604325

  1. Simultaneous spatiotemporal mapping of in situ pH and bacterial activity within an intact 3D microcolony structure

    PubMed Central

    Hwang, Geelsu; Liu, Yuan; Kim, Dongyeop; Sun, Victor; Aviles-Reyes, Alejandro; Kajfasz, Jessica K.; Lemos, Jose A.; Koo, Hyun

    2016-01-01

    Biofilms are comprised of bacterial-clusters (microcolonies) enmeshed in an extracellular matrix. Streptococcus mutans can produce exopolysaccharides (EPS)-matrix and assemble microcolonies with acidic microenvironments that can cause tooth-decay despite the surrounding neutral-pH found in oral cavity. How the matrix influences the pH and bacterial activity locally remains unclear. Here, we simultaneously analyzed in situ pH and gene expression within intact biofilms and measured the impact of damage to the surrounding EPS-matrix. The spatiotemporal changes of these properties were characterized at a single-microcolony level following incubation in neutral-pH buffer. The middle and bottom-regions as well as inner-section within the microcolony 3D structure were resistant to neutralization (vs. upper and peripheral-region), forming an acidic core. Concomitantly, we used a green fluorescent protein (GFP) reporter to monitor expression of the pH-responsive atpB (PatpB::gfp) by S. mutans within microcolonies. The atpB expression was induced in the acidic core, but sharply decreased at peripheral/upper microcolony regions, congruent with local pH microenvironment. Enzymatic digestion of the surrounding matrix resulted in nearly complete neutralization of microcolony interior and down-regulation of atpB. Altogether, our data reveal that biofilm matrix facilitates formation of an acidic core within microcolonies which in turn activates S. mutans acid-stress response, mediating both the local environment and bacterial activity in situ. PMID:27604325

  2. Active Regions on the Farside of the Sun as Seen from Mars: 3D Visualization with Marie Data

    NASA Technical Reports Server (NTRS)

    Saganti, P. B.; Cueinotra, F. A.; Cleghorn, T. F.; Zeitlin, C. J.

    2004-01-01

    From March 2002, the MARIE (Martian Radiation Environment Experiment) instrument of NASA-JSC onboard the 2001 Mars Odyssey spacecraft has been providing radiation data from the Martian orbit. During the past two years, the orbit alignment of Mars-Sun-Earth provided a wealth of opportunity between 180 degrees (August 2002) and 0 degrees (October 2003). During this time, the MARIE data included the background GCR (Galactic Cosmic Rays) and several SPE (Solar Particle Events) enhanced radiation dose-rate measurements at Mars. The MARIE instrument provided a unique data set of radiation dose-rate at Mars from the active regions on the solar disk facing the Mars side. The SPE observations of October 2002 at Mars by the MARIE instrument are unique and there were no indications of these events towards the Earth at that time. Nearly 40 times increase in the quiet-time GCR dose-rate was noted from about 25 mradday to nearly 1000 mradday at Mars. Radiation dose-rate enhancement was not observed toward the Earth or in the Low Earth Orbit (LEO) during this time. Understanding the active regions on the Sun that are likely to result into SPE on the far side will also be of concern for future deep space explorations beyond LEO. We present the observations of these SPE enhanced dose rates due to the active regions from the far side of the Sun with the 3D visualization of solar disks facing Mars and Earth.

  3. Short and long time effects of low temperature Plasma Activated Media on 3D multicellular tumor spheroids.

    PubMed

    Judée, Florian; Fongia, Céline; Ducommun, Bernard; Yousfi, Mohammed; Lobjois, Valérie; Merbahi, Nofel

    2016-01-01

    This work investigates the regionalized antiproliferative effects of plasma-activated medium (PAM) on colon adenocarcinoma multicellular tumor spheroid (MCTS), a model that mimics 3D organization and regionalization of a microtumor region. PAM was generated by dielectric barrier plasma jet setup crossed by helium carrier gas. MCTS were transferred in PAM at various times after plasma exposure up to 48 hours and effect on MCTS growth and DNA damage were evaluated. We report the impact of plasma exposure duration and delay before transfer on MCTS growth and DNA damage. Local accumulation of DNA damage revealed by histone H2AX phosphorylation is observed on outermost layers and is dependent on plasma exposure. DNA damage is completely reverted by catalase addition indicating that H2O2 plays major role in observed genotoxic effect while growth inhibitory effect is maintained suggesting that it is due to others reactive species. SOD and D-mannitol scavengers also reduced DNA damage by 30% indicating that O(2)(-)* and OH* are involved in H2O2 formation. Finally, PAM is able to retain its cytotoxic and genotoxic activity upon storage at +4 °C or -80 °C. These results suggest that plasma activated media may be a promising new antitumor strategy for colorectal cancer tumors. PMID:26898904

  4. Stumbling reactions during perturbed walking: Neuromuscular reflex activity and 3-D kinematics of the trunk - A pilot study.

    PubMed

    Müller, Juliane; Müller, Steffen; Engel, Tilman; Reschke, Antje; Baur, Heiner; Mayer, Frank

    2016-04-11

    Reflex activity of the lower leg muscles involved when compensating for falls has already been thoroughly investigated. However, the trunk׳s role in this compensation strategy remains unclear. The purpose of this study, therefore, was to analyze the kinematics and muscle activity of the trunk during perturbed walking. Ten subjects (29±3yr;79±11cm;74±14kg) walked (1m/s) on a split-belt treadmill, while 5 randomly timed, right-sided perturbations (treadmill belt deceleration: 40m/s(2)) were applied. Trunk muscle activity was assessed with a 12-lead-EMG. Trunk kinematics were measured with a 3D-motion analysis system (12 markers framing 3 segments: upper thoracic area (UTA), lower thoracic area (LTA), lumbar area (LA)). The EMG-RMS [%] (0-200ms after perturbation) was analyzed and then normalized to the RMS of normal walking. The total range of motion (ROM;[°]) for the extension/flexion, lateral flexion and rotation of each segment were calculated. Individual kinematic differences between walking and stumbling [%; ROM] were also computed. Data analysis was conducted descriptively, followed by one- and two-way ANOVAs (α=0.05). Stumbling led to an increase in ROM, compared to unperturbed gait, in all segments and planes. These increases ranged between 107±26% (UTA/rotation) and 262±132% (UTS/lateral flexion), significant only in lateral flexion. EMG activity of the trunk was increased during stumbling (abdominal: 665±283%; back: 501±215%), without significant differences between muscles. Provoked stumbling leads to a measurable effect on the trunk, quantifiable by an increase in ROM and EMG activity, compared to normal walking. Greater abdominal muscle activity and ROM of lateral flexion may indicate a specific compensation pattern occurring during stumbling. PMID:26518368

  5. Synthesis and antiviral activity of certain 4-substituted and 2,4-disubstituted 7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3-d]pyrimidines.

    PubMed

    Saxena, N K; Hagenow, B M; Genzlinger, G; Turk, S R; Drach, J C; Townsend, L B

    1988-08-01

    Treatment of the sodium salt of 4-chloro-2-(methylthio)pyrrolo[2,3-d]pyrimidine (2) with (2-acetoxyethoxy)methyl bromide (3) has provided 4-chloro-2-(methylthio)-7[(2-acetoxyethoxy)methyl]pyrrolo[2,3- d]pyrimidine (4). Ammonolysis of 4 at room temperature gave 4-chloro-2-(methylthio)-7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3- d]pyrimidine (5). However, ammonolysis of 5 at 130 degrees C furnished 4-amino-2-(methylthio)-7-[(2-hydroxyethoxy)methyl]-pyrrolo[2,3- d]pyrimidine (6), which on desulfurization with Raney Ni yielded 4-amino-7-[(2-hydroxyethoxy)-methyl]pyrrolo[2,3-d]pyrimidine (7) (acyclic analogue of tubercidin). The oxidation of 6 with m-chloroperbenzoic acid provided the sulfone derivative 8. A nucleophilic displacement of the 2-methylsulfonyl group from 8 with methoxide anion provided 4-amino-2-methoxy-7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3-d]pyrimidine (9). Demethylation of 9 with iodotrimethylsilane gave 4-amino-2-hydroxy-7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3-d]pyrimidine (10). Treatment of 2,4-dichloropyrrolo[2,3-d]pyrimidine (11) with 3 gave the protected acyclic compound 12, which on deacetylation and ammonolysis under controlled reaction conditions gave 2,4-dichloro-7-[(2-hydroxyethoxy)-methyl]pyrrolo[2,3-d]pyrimidine (13) and 4-amino-2-chloro-7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3- d]pyrimidine (14), respectively. The condensation of 2-acetamido-4-chloropyrrolo[2,3-d]pyrimidine (15) with 3 gave the protected acyclic compound 16, which on concomitant deacetylation and ammonolysis with methanolic ammonia at an elevated temperature yielded 2,4-diamino-7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3-d]pyrimidine (17) in moderate yield. In tests involving human cytomegalovirus (HCMV) and herpes simplex virus type 1 (HSV-1), only slight activity and cytotoxicity were observed. The most active compounds (12 and 13) were slightly more active against HCMV than acyclovir, but both compounds were inactive against HSV-1. The activity against HCMV, however, was not well

  6. Comparative proteomic analyses demonstrate enhanced interferon and STAT-1 activation in reovirus T3D-infected HeLa cells

    PubMed Central

    Ezzati, Peyman; Komher, Krysten; Severini, Giulia; Coombs, Kevin M.

    2015-01-01

    As obligate intracellular parasites, viruses are exclusively and intimately dependent upon their host cells for replication. During replication viruses induce profound changes within cells, including: induction of signaling pathways, morphological changes, and cell death. Many such cellular perturbations have been analyzed at the transcriptomic level by gene arrays and recent efforts have begun to analyze cellular proteomic responses. We recently described comparative stable isotopic (SILAC) analyses of reovirus, strain type 3 Dearing (T3D)-infected HeLa cells. For the present study we employed the complementary labeling strategy of iTRAQ (isobaric tags for relative and absolute quantitation) to examine HeLa cell changes induced by T3D, another reovirus strain, type 1 Lang, and UV-inactivated T3D (UV-T3D). Triplicate replicates of cytosolic and nuclear fractions identified a total of 2375 proteins, of which 50, 57, and 46 were significantly up-regulated, and 37, 26, and 44 were significantly down-regulated by T1L, T3D, and UV-T3D, respectively. Several pathways, most notably the Interferon signaling pathway and the EIF2 and ILK signaling pathways, were induced by virus infection. Western blots confirmed that cells were more strongly activated by live T3D as demonstrated by elevated levels of key proteins like STAT-1, ISG-15, IFIT-1, IFIT-3, and Mx1. This study expands our understanding of reovirus-induced host responses. PMID:25905045

  7. Temporal monitoring and quantification of hydrothermal activity from photomosaics and 3D video reconstruction: The Lucky Strike hydrothermal field

    NASA Astrophysics Data System (ADS)

    Barreyre, T.; Escartin, J.; Cannat, M.; Garcia, R. A.

    2011-12-01

    Seafloor imagery provides detailed and accurate constrain on the distribution, geometry, and nature of hydrothermal outflow, and its links to the ecosystems that they sustain. Repeated surveys allow us to evaluate the temporal variability of these systems. Geo-referenced and co-registered photomosaics of the Lucky Strike hydrothermal field (Mid Atlantic Ridge, 37°N), derived from >60,000 seafloor images acquired in 1996, 2006, 2008 and 2009, using deep-towed and ROV vehicles. Newly-developed image processing techniques, specifically tailored to generate giga-mosaics in the underwater environment, include correction of illumination artifacts and removal of the edges between individual images so as to obtain a continuous and single mosaic image over a surface of up ~800x800 m and with a pixel resolution of 5-10 mm. Photomosaicing is complemented by 3D-reconstruction of hydrothermal edifices from video imagery, with the mapping of image texture over the 3D model surface. These image and video data can also be directly linked with high-resolution microbathymetry acquired near-bottom acoustic systems. Preliminary analysis of these mosaics reveals the distribution of low-temperature hydrothermal outflow, recognizable owing to its association with bacterial mats and hydrothermal deposits easily identifiable in the imagery. These low-temperature venting areas, often associated with high-temperature hydrothermal vents, are irregularly distributed throughout the site, defining clusters. In detail, the outflow geometry is largely controlled by the nature of the substrate (e.g., cracks and fissures, diffuse flow patches, existing hydrothermal constructs). The spatial relationships between the high- and diffuse venting as revealed by the imagery provide constraints on the shallow plumbing structure throughout the site.. Imagery provides constraints on temporal variability at two time-scales. First, we can identify changes in the distribution and presence of actively venting

  8. Potent activation of dopamine D3/D2 heterodimers by the antiparkinsonian agents, S32504, pramipexole and ropinirole.

    PubMed

    Maggio, Roberto; Scarselli, Marco; Novi, Francesca; Millan, Mark J; Corsini, Giovanni U

    2003-11-01

    Recombinant, human dopamine D3 and D2 receptors form functional heterodimers upon co-expression in COS-7 cells. Herein, actions of the antiparkinsonian agents, S32504, ropinirole and pramipexole, at D3/D2L heterodimers were compared to their effects at the respective monomers and at split, chimeric D3trunk/D2tail and D2trunk/D3tail receptors: the trunk incorporated transmembrane domains (TDs) I-V and the tail TDs VI and VII. In binding assays with the antagonist [3H]nemonapride, all agonists were potent ligands of D3 receptors showing, respectively, 100-, 18- and 56-fold lower affinity at D2L receptors, mimicking the selective D3 receptor antagonist, S33084 (100-fold). At D3trunk/D2tail receptors, except for ropinirole, all drugs showed lower affinities than at D3 sites, whereas for D2trunk/D3tail receptors, affinities of all drugs were higher than at D2L sites. The proportion of high affinity binding sites recognized by S32504, pramipexole and ropinirole in membranes derived from cells co-expressing D3 and D2L sites was higher than in an equivalent mixture of membranes from cells expressing D3 or D2L sites, consistent with the promotion of heterodimer formation. In contrast, the percentage of high and low affinity sites (biphasic isotherms) recognized by S33084 was identical. Functional actions were determined by co-transfection of a chimeric adenylyl cyclase (AC)-V/VI insensitive to D3 receptors. Accordingly, D3 receptor-transfected cells were irresponsive whereas, in D2L receptor-transfected cells, agonists suppressed forskolin-stimulated cAMP production with modest potencies. In cells co-transfected with D3 and D2L receptors, S32504, ropinirole and pramipexole potently suppressed AC-V/VI with EC50s 33-, 19- and 11-fold lower than at D2L receptors, respectively. S32504 also suppressed AC-V/VI activity at split D3trunk/D2tail and D2trunk/D3tail chimeras transfected into COS-7 cells. In conclusion, antiparkinson agents behave as potent agonists at D3/D2

  9. Land use influence on 3-D distribution of soil microbiological activity in forest-steppe zone of Central Russia

    NASA Astrophysics Data System (ADS)

    Vasenev, Viacheslav; Tembo, Alan; Sarzhanov, Dmirty; Sotnikova, Julia; Ryzhkov, Oleg; Lakeev, Pavel; Valentini, Riccardo

    2014-05-01

    Land use is the principal factor influencing soil environmental functions and quality. Quite a few studies on soil quality mainly focus on natural and agroecosystems. Much less is known about urban ecosystems, although the urbanization effect on soil quality can be considerable. Parameters of soil microbiological activity are very sensitive to land-use change. Microbial biomass carbon (Cmic), basal respiration (BR) and microbial metabolic coefficient (qCO2) are among most widely used parameters of soil microbiological activity. They are directly associated with such soil functions as fertility, microorganisms' habitat and participation in carbon cycle. So far, most of the studies focus on the effect of land-use change on the topsoil (0-10 cm) microbiological activity, averaged for different land-use types. Much less is known about changes in spatial variability and profile distribution of Cmic, BR and qCO2 in response to different land-use. Land-use influence on spatial and profile distribution of soil microbiological activity may differ between bioclimatic zones. Very fertile and rich in carbon Chernozemic soils (depth of the A horizon up to 1 m, carbon concentration up to 7-9%), dominating in forest-steppe zone are among the most sensitive to land-use change. This study aims to improve understanding of land-use influence on 3-D distribution of Cmic, BR and qCO2 in Central Chernozemic region of Russia. We observed three land-use types (fallow land, natural pasture and meadow) located in Kursk region and three contrast urban functional zone (industrial, residential and recreational) in Kursk city. Soil samples were collected by auguring in five replicas per land-use type, four layers each sampling point (0-10, 10-50, 50-100 and 100-150 cm). Cmic, BR and qCO2 as well as Corg, N and pHKCl were analyzed in all the samples. Cmic (µg C g-1 soil) was analyzed based on the substrate induced respiration (SIR) approach. qCO2 (μg CO2-C mg-1 Cmic h-1) was calculated as the

  10. Local and systemic activation of the whole complement cascade in human leukocytoclastic cutaneous vasculitis; C3d,g and terminal complement complex as sensitive markers.

    PubMed Central

    Dauchel, H; Joly, P; Delpech, A; Thomine, E; Sauger, F; Le Loet, X; Lauret, P; Tron, F; Fontaine, M; Ripoche, J

    1993-01-01

    We have studied complement activation both in plasma samples and in lesional skin from patients with leukocytoclastic cutaneous vasculitis (LCV). Enzyme immunoassay (EIA) quantification of the complement activation markers, C3d,g and the terminal complement complex (TCC) in plasma, showed that their levels were significantly increased in 66% and 55% of the patients, respectively (n = 29) compared with healthy controls, whereas the standard measurements of C3, factor B, C1q, C4 and C2 were generally within normal range. Elevations of C3d,g and TCC levels in plasma were significantly correlated. Importantly, a significant correlation was found between the severity of the vasculitis and both C3d,g and TCC plasma levels. Immunofluorescence studies of skin biopsy specimens demonstrated simultaneous presence of perivascular dermal deposits of C3d,g and TCC in lesional skin from 96% and 80% respectively of the patients (n = 25). There was a significant correlation between the intensity of the deposits of both markers. Clusterin, a TCC inhibitory protein, was always found at the same sites of perivascular TCC deposits. Immunofluorescence studies at the epidermal basement membrane zone (BMZ) revealed in each case deposits of C3d,g which were accompanied by TCC deposits in 52% of the biopsy specimens. These data demonstrate that there is a local and systemic activation of the whole complement cascade in human LCV. The presence of both C3d,g and clusterin-associated TCC perivascular deposits suggests an intervention of a regulatory mechanism of local complement activation in LCV. Finally, measurement of plasma C3d,g and TCC appears to be a sensitive indicator of systemic complement activation and disease severity in LCV. Images Fig. 1 Fig. 2 PMID:8485913

  11. Cliffs' GRE StudyWare Package: A Critical Evaluation.

    ERIC Educational Resources Information Center

    Brooke, Stephanie L.

    1995-01-01

    Provides evaluation of Cliffs' GRE StudyWare package (Bobrow, 1992). Discusses the educational implications of using Cliffs' approach, in addition to focusing on software considerations. Makes recommendations concerning Cliffs' method for Graduate Record Examination (GRE) preparation. (Author/LKS)

  12. Active remote sensing of snow using NMM3D/DMRT and comparison with CLPX II airborne data

    USGS Publications Warehouse

    Xu, X.; Liang, D.; Tsang, L.; Andreadis, K.M.; Josberger, E.G.; Lettenmaier, D.P.; Cline, D.W.; Yueh, S.H.

    2010-01-01

    We applied the Numerical Maxwell Model of three-dimensional simulations (NMM3D) in the Dense Media Radiative Theory (DMRT) to calculate backscattering coefficients. The particles' positions are computer-generated and the subsequent Foldy-Lax equations solved numerically. The phase matrix in NMM3D has significant cross-polarization, particularly when the particles are densely packed. The NMM3D model is combined with DMRT in calculating the microwave scattering by dry snow. The NMM3D/DMRT equations are solved by an iterative solution up to the second order in the case of small to moderate optical thickness. The numerical results of NMM3D/DMRT are illustrated and compared with QCA/DMRT. The QCA/DMRT and NMM3D/DMRT results are also applied to compare with data from two specific datasets from the second Cold Land Processes Experiment (CLPX II) in Alaska and Colorado. The data are obtained at the Ku-band (13.95 GHz) observations using airborne imaging polarimetric scatterometer (POLSCAT). It is shown that the model predictions agree with the field measurements for both co-polarization and cross-polarization. For the Alaska region, the average snow depth and snow density are used as the inputs for DMRT. The grain size, selected from within the range of the ground measurements, is used as a best-fit parameter within the range. For the Colorado region, we use the Variable Infiltration Capacity Model (VIC) to obtain the input snow profiles for NMM3D/DMRT. ?? 2010 IEEE.

  13. 3D Modelling of Inaccessible Areas using UAV-based Aerial Photography and Structure from Motion

    NASA Astrophysics Data System (ADS)

    Obanawa, Hiroyuki; Hayakawa, Yuichi; Gomez, Christopher

    2014-05-01

    In hardly accessible areas, the collection of 3D point-clouds using TLS (Terrestrial Laser Scanner) can be very challenging, while airborne equivalent would not give a correct account of subvertical features and concave geometries like caves. To solve such problem, the authors have experimented an aerial photography based SfM (Structure from Motion) technique on a 'peninsular-rock' surrounded on three sides by the sea at a Pacific coast in eastern Japan. The research was carried out using UAS (Unmanned Aerial System) combined with a commercial small UAV (Unmanned Aerial Vehicle) carrying a compact camera. The UAV is a DJI PHANTOM: the UAV has four rotors (quadcopter), it has a weight of 1000 g, a payload of 400 g and a maximum flight time of 15 minutes. The camera is a GoPro 'HERO3 Black Edition': resolution 12 million pixels; weight 74 g; and 0.5 sec. interval-shot. The 3D model has been constructed by digital photogrammetry using a commercial SfM software, Agisoft PhotoScan Professional®, which can generate sparse and dense point-clouds, from which polygonal models and orthophotographs can be calculated. Using the 'flight-log' and/or GCPs (Ground Control Points), the software can generate digital surface model. As a result, high-resolution aerial orthophotographs and a 3D model were obtained. The results have shown that it was possible to survey the sea cliff and the wave cut-bench, which are unobservable from land side. In details, we could observe the complexity of the sea cliff that is nearly vertical as a whole while slightly overhanging over the thinner base. The wave cut bench is nearly flat and develops extensively at the base of the cliff. Although there are some evidences of small rockfalls at the upper part of the cliff, there is no evidence of very recent activity, because no fallen rock exists on the wave cut bench. This system has several merits: firstly lower cost than the existing measuring methods such as manned-flight survey and aerial laser

  14. Europeana and 3D

    NASA Astrophysics Data System (ADS)

    Pletinckx, D.

    2011-09-01

    The current 3D hype creates a lot of interest in 3D. People go to 3D movies, but are we ready to use 3D in our homes, in our offices, in our communication? Are we ready to deliver real 3D to a general public and use interactive 3D in a meaningful way to enjoy, learn, communicate? The CARARE project is realising this for the moment in the domain of monuments and archaeology, so that real 3D of archaeological sites and European monuments will be available to the general public by 2012. There are several aspects to this endeavour. First of all is the technical aspect of flawlessly delivering 3D content over all platforms and operating systems, without installing software. We have currently a working solution in PDF, but HTML5 will probably be the future. Secondly, there is still little knowledge on how to create 3D learning objects, 3D tourist information or 3D scholarly communication. We are still in a prototype phase when it comes to integrate 3D objects in physical or virtual museums. Nevertheless, Europeana has a tremendous potential as a multi-facetted virtual museum. Finally, 3D has a large potential to act as a hub of information, linking to related 2D imagery, texts, video, sound. We describe how to create such rich, explorable 3D objects that can be used intuitively by the generic Europeana user and what metadata is needed to support the semantic linking.

  15. 3D crustal-scale heat-flow regimes at a developing active margin (Taranaki Basin, New Zealand)

    NASA Astrophysics Data System (ADS)

    Kroeger, K. F.; Funnell, R. H.; Nicol, A.; Fohrmann, M.; Bland, K. J.; King, P. R.

    2013-04-01

    The Taranaki Basin in the west of New Zealand's North Island has evolved from a rifted Mesozoic Gondwana margin to a basin straddling the Neogene convergent Australian-Pacific plate margin. However, given its proximity to the modern subduction front, Taranaki Basin is surprisingly cold when compared to other convergent margins. To investigate the effects of active margin evolution on the thermal regime of the Taranaki Basin we developed a 3D crustal-scale forward model using the petroleum industry-standard basin-modelling software Petromod™. The crustal structure inherited from Mesozoic Gondwana margin breakup and processes related to modern Hikurangi convergent margin initiation are identified to be the main controls on the thermal regime of the Taranaki Basin. Present-day surface heat flow across Taranaki on average is 59 mW/m2, but varies by as much as 30 mW/m2 due to the difference in crustal heat generation between mafic and felsic basement terranes alone. In addition, changes in mantle heat advection, tectonic subsidence, crustal thickening and basin inversion, together with related sedimentary processes result in variability of up to 10 mW/m2. Modelling suggests that increased heating of the upper crust due to additional mantle heat advection following the onset of subduction is an ongoing process and heating has only recently begun to reach the surface, explaining the relatively low surface heat flow. We propose that the depth of the subducted slab and related mantle convection processes control the thermal and structural regimes in the Taranaki Basin. The thermal effects of the subduction initiation process are modified and overprinted by the thickness, structure and composition of the lithosphere.

  16. 3D Geometry of Active Shortening, Uplift and Subsidence West of the Alpine Fault (South Island, New Zealand)

    NASA Astrophysics Data System (ADS)

    Ghisetti, F.; Sibson, R. H.; Hamling, I. J.

    2014-12-01

    The Alpine Fault is the principal component of the transform boundary between the Australian and Pacific plates across the South Island of New Zealand, linking the opposite dipping Hikurangi and Puysegur subduction zones. In the northern South Island, the transition from the subducted W-dipping Pacific slab of the Hikurangi margin to the intra-continental transform margin is defined by earthquake foci from 350 to 100 km deep. West of the Alpine Fault the Australian crust above the slab has been incorporated into the collisional plate boundary and uplifted in a compressional belt up to 100 km wide. Retro-deformation and back-stripping of 10 regional transects utilising surface geology, seismic reflection lines and exploration wells define the progressive deformation of the Australian crust since 35 Ma along the collisional margin. The reconstructed geometry of faulted basement blocks is tied to localisation and evolution of overlying sedimentary basins, coeval with displacement on the Alpine Fault. Amounts of shortening, uplift and subsidence and fault activity are heterogeneous in space and time across the margin, and are controlled by compressional reactivation of inherited high-angle, N-S Paleogene normal faults oblique to the margin. However, significant differences also occur along the strike of the collisional margin, with major contrasts in uplift and subsidence north and south of lat. 41°.7, i.e. the region overlying the southern termination of the Hikurangi slab. These differences are highlighted by present day hydrographic anomalies in the Buller region, and by the pattern of filtered topography at > 75 km wavelength. Our data show that the 3D geometry of the Australian plate cannot be entirely attributed to inherited crustal heterogeneity of a flexured "retro-foreland" domain in the footwall of the Alpine Fault, and suggest the need of deeper dynamic interaction between the Pacific and Australian lithosphere along the subduction-collision margin.

  17. 3D MR-Spectroscopic Imaging Assessment of Metabolic Activity in the Prostate During the PSA 'Bounce' Following {sup 125}Iodine Brachytherapy

    SciTech Connect

    Kirilova, Anna; Damyanovich, Andrei; Crook, Juanita; Jezioranski, John; Wallace, Kris; Pintilie, Melania

    2011-02-01

    Purpose: A temporary increase in prostate-specific antigen (PSA) values is observed in 30%-40% of men following {sup 125} I brachytherapy (BT) for prostate cancer. We present the results of a study to characterize prostate metabolic activity during the PSA 'bounce' and to correlate metabolic changes with PSA levels using three-dimensional magnetic resonance spectroscopic imaging (3D-MRSI). Methods and Materials: 3D-MRSI was performed in 24 patients during the PSA bounce. Eight of these had also had a baseline 3D-MRSI scan before BT for the purpose of tumor mapping. The 3D-MRSI was repeated at 6- and 12-month intervals, and PSA levels were monitored every 3 months. Twenty-one of the patients had favorable-risk prostate cancer, and 3 had intermediate risk. Results: The choline+creatine signal intensity, although markedly reduced, was observable following BT. Diffuse activity not corresponding to original biopsy-positive sites was observed in 22 cases, and 2 cases were documented to have local recurrence. No statistically significant correlation between metabolic activity and PSA levels at each interval was found. Conclusion: Post-BT prostate 3D-MRSI shows evidence of diffuse metabolic activity unrelated to residual malignancy. This supports the benign nature of the PSA bounce and suggests an inflammatory etiology. In the situation of a rising PSA, observation of focal activity on MRI/3D-MRSI could be a useful adjunct to suggest local recurrence at an earlier interval after brachytherapy when prostate biopsies would still be unhelpful. Longer follow-up is necessary to confirm the complex relationship between metabolic activity and PSA levels.

  18. 3D active edge silicon sensors: Device processing, yield and QA for the ATLAS-IBL production

    NASA Astrophysics Data System (ADS)

    Da Vià, Cinzia; Boscardil, Maurizio; Dalla Betta, GianFranco; Darbo, Giovanni; Fleta, Celeste; Gemme, Claudia; Giacomini, Gabriele; Grenier, Philippe; Grinstein, Sebastian; Hansen, Thor-Erik; Hasi, Jasmine; Kenney, Christopher; Kok, Angela; La Rosa, Alessandro; Micelli, Andrea; Parker, Sherwood; Pellegrini, Giulio; Pohl, David-Leon; Povoli, Marco; Vianello, Elisa; Zorzi, Nicola; Watts, S. J.

    2013-01-01

    3D silicon sensors, where plasma micromachining is used to etch deep narrow apertures in the silicon substrate to form electrodes of PIN junctions, were successfully manufactured in facilities in Europe and USA. In 2011 the technology underwent a qualification process to establish its maturity for a medium scale production for the construction of a pixel layer for vertex detection, the Insertable B-Layer (IBL) at the CERN-LHC ATLAS experiment. The IBL collaboration, following that recommendation from the review panel, decided to complete the production of planar and 3D sensors and endorsed the proposal to build enough modules for a mixed IBL sensor scenario where 25% of 3D modules populate the forward and backward part of each stave. The production of planar sensors will also allow coverage of 100% of the IBL, in case that option was required. This paper will describe the processing strategy which allowed successful 3D sensor production, some of the Quality Assurance (QA) tests performed during the pre-production phase and the production yield to date.

  19. 3d-3d correspondence revisited

    NASA Astrophysics Data System (ADS)

    Chung, Hee-Joong; Dimofte, Tudor; Gukov, Sergei; Sułkowski, Piotr

    2016-04-01

    In fivebrane compactifications on 3-manifolds, we point out the importance of all flat connections in the proper definition of the effective 3d {N}=2 theory. The Lagrangians of some theories with the desired properties can be constructed with the help of homological knot invariants that categorify colored Jones polynomials. Higgsing the full 3d theories constructed this way recovers theories found previously by Dimofte-Gaiotto-Gukov. We also consider the cutting and gluing of 3-manifolds along smooth boundaries and the role played by all flat connections in this operation.

  20. A new 3-D open-framework cadmium borovanadate with plane-shaped channels and high catalytic activity for the oxidation of cyclohexanol.

    PubMed

    Feng, Yuquan; Qiu, Dongfang; Fan, Huitao; Li, Min; Huang, Qunzeng; Shi, Hengzhen

    2015-05-21

    A new 3-D open-framework cadmium borovanadate with 6-connected topology was hydrothermally obtained and structurally characterized. It not only features new cadmium(II) borovanadate which possesses an open-framework structure with unique plane-shaped channels, but also exhibits interesting absorption properties and high catalytic activities for the oxidation of cyclohexanol. PMID:25882921

  1. 3D SMoSIFT: three-dimensional sparse motion scale invariant feature transform for activity recognition from RGB-D videos

    NASA Astrophysics Data System (ADS)

    Wan, Jun; Ruan, Qiuqi; Li, Wei; An, Gaoyun; Zhao, Ruizhen

    2014-03-01

    Human activity recognition based on RGB-D data has received more attention in recent years. We propose a spatiotemporal feature named three-dimensional (3D) sparse motion scale-invariant feature transform (SIFT) from RGB-D data for activity recognition. First, we build pyramids as scale space for each RGB and depth frame, and then use Shi-Tomasi corner detector and sparse optical flow to quickly detect and track robust keypoints around the motion pattern in the scale space. Subsequently, local patches around keypoints, which are extracted from RGB-D data, are used to build 3D gradient and motion spaces. Then SIFT-like descriptors are calculated on both 3D spaces, respectively. The proposed feature is invariant to scale, transition, and partial occlusions. More importantly, the running time of the proposed feature is fast so that it is well-suited for real-time applications. We have evaluated the proposed feature under a bag of words model on three public RGB-D datasets: one-shot learning Chalearn Gesture Dataset, Cornell Activity Dataset-60, and MSR Daily Activity 3D dataset. Experimental results show that the proposed feature outperforms other spatiotemporal features and are comparative to other state-of-the-art approaches, even though there is only one training sample for each class.

  2. Correlation of C3d fixing circulating immune complexes with disease activity and clinical parameters in patients with systemic lupus erythematosus.

    PubMed Central

    Sekita, K; Doi, T; Muso, E; Yoshida, H; Kanatsu, K; Hamashima, Y

    1984-01-01

    Using anti-C3d as a solid phase reagent, C3d fixing circulating immune complexes (CIC) were detected in sera from patients with systemic lupus erythematosus (SLE), rheumatoid arthritis, membranous nephropathy and IgA nephropathy. Particularly, sera from SLE showed the highest CIC levels and highest incidence of positivity among these diseases. In the 51 serum samples from 48 patients with SLE we studied, the CIC detected by the anti-C3d assay correlated well (P less than 0.01) with the CIC detected by the solid phase C1q assay, but not with those detected by the conglutinin assay. In addition, the CIC detected by the anti-C3d assay correlated more significantly (P less than 0.001) with disease activity, as well as some clinical parameters (serum anti-dsDNA antibodies, CH50 and C3 levels) than CIC detected by the other two assays of SLE sera. The anti-C3d binding materials were found to be of intermediate (8-19S) and small (7S) sizes in a small number of SLE sera which we analysed. PMID:6608422

  3. Direct growth of single-crystal Pt nanowires on Sn@CNT Nanocable: 3D electrodes for highly active electrocatalysts.

    PubMed

    Sun, Shuhui; Zhang, Gaixia; Geng, Dongsheng; Chen, Yougui; Banis, Mohammad Norouzi; Li, Ruying; Cai, Mei; Sun, Xueliang

    2010-01-18

    A newly designed and fabricated novel three dimensional (3D) nanocomposite composed of single-crystal Pt nanowires (PtNW) and a coaxial nanocable support consisting of a tin nanowire and a carbon nanotube (Sn@CNT) is reported. This nanocomposite is fabricated by the synthesis of Sn@CNT nanocables by means of a thermal evaporation method, followed by the direct growth with PtNWs through a facile aqueous solution approach at room temperature. Electrochemical measurements demonstrate that the PtNW--Sn@CNT 3D electrode exhibits enhanced electrocatalytic performance in oxygen reduction reaction (ORR) for polymer electrolyte membrane fuel cells (PEMFCs), methanol oxidation (MOR) for direct methanol fuel cells (DMFCs), and CO tolerance compared with commercial ETEK Pt/C catalyst made of Pt nanoparticles. PMID:20024993

  4. A hybrid framework of multiple active appearance models and global registration for 3D prostate segmentation in MRI

    NASA Astrophysics Data System (ADS)

    Ghose, Soumya; Oliver, Arnau; Martí, Robert; Lladó, Xavier; Freixenet, Jordi; Mitra, Jhimli; Vilanova, Joan C.; Meriaudeau, Fabrice

    2012-02-01

    Real-time fusion of Magnetic Resonance (MR) and Trans Rectal Ultra Sound (TRUS) images aid in the localization of malignant tissues in TRUS guided prostate biopsy. Registration performed on segmented contours of the prostate reduces computational complexity and improves the multimodal registration accuracy. However, accurate and computationally efficient 3D segmentation of the prostate in MR images could be a challenging task due to inter-patient shape and intensity variability of the prostate gland. In this work, we propose to use multiple statistical shape and appearance models to segment the prostate in 2D and a global registration framework to impose shape restriction in 3D. Multiple mean parametric models of the shape and appearance corresponding to the apex, central and base regions of the prostate gland are derived from principal component analysis (PCA) of prior shape and intensity information of the prostate from the training data. The estimated parameters are then modified with the prior knowledge of the optimization space to achieve segmentation in 2D. The 2D segmented slices are then rigidly registered with the average 3D model produced by affine registration of the ground truth of the training datasets to minimize pose variations and impose 3D shape restriction. The proposed method achieves a mean Dice similarity coefficient (DSC) value of 0.88+/-0.11, and mean Hausdorff distance (HD) of 3.38+/-2.81 mm when validated with 15 prostate volumes of a public dataset in leave-one-out validation framework. The results achieved are better compared to some of the works in the literature.

  5. Intelligent robots and computer vision XII: Active vision and 3D methods; Proceedings of the Meeting, Boston, MA, Sept. 8, 9, 1993

    SciTech Connect

    Casasent, D.P.

    1993-01-01

    Topics addressed include active vision for intelligent robots, 3D vision methods, tracking in robotic and vision, visual servoing and egomotion in robotics, egomotion and time-sequential processing, and control and planning in robotics and vision. Particular attention is given to invariant in visual motion, generic target tracking using color, recognizing 3D articulated-line-drawing objects, range data acquisition from an encoded structured light pattern, and 3D edge orientation detection. Also discussed are acquisition of randomly moving objects by visual guidance, fundamental principles of robot vision, high-performance visual servoing for robot end-point control, a long sequence analysis of human motion using eigenvector decomposition, and sequential computer algorithms for printed circuit board inspection.

  6. HER2 signaling pathway activation and response of breast cancer cells to HER2-targeting agents is dependent strongly on the 3D microenvironment

    SciTech Connect

    Weigelt, Britta; Lo, Alvin T; Park, Catherine C; Gray, Joe W; Bissell, Mina J

    2009-07-27

    Development of effective and durable breast cancer treatment strategies requires a mechanistic understanding of the influence of the microenvironment on response. Previous work has shown that cellular signaling pathways and cell morphology are dramatically influenced by three-dimensional (3D) cultures as opposed to traditional two-dimensional (2D) monolayers. Here, we compared 2D and 3D culture models to determine the impact of 3D architecture and extracellular matrix (ECM) on HER2 signaling and on the response of HER2-amplified breast cancer cell lines to the HER2-targeting agents Trastuzumab, Pertuzumab and Lapatinib. We show that the response of the HER2-amplified AU565, SKBR3 and HCC1569 cells to these anti-HER2 agents was highly dependent on whether the cells were cultured in 2D monolayer or 3D laminin-rich ECM gels. Inhibition of {beta}1 integrin, a major cell-ECM receptor subunit, significantly increased the sensitivity of the HER2-amplified breast cancer cell lines to the humanized monoclonal antibodies Trastuzumab and Pertuzumab when grown in a 3D environment. Finally, in the absence of inhibitors, 3D cultures had substantial impact on HER2 downstream signaling and induced a switch between PI3K-AKT- and RAS-MAPKpathway activation in all cell lines studied, including cells lacking HER2 amplification and overexpression. Our data provide direct evidence that breast cancer cells are able to rapidly adapt to different environments and signaling cues by activating alternative pathways that regulate proliferation and cell survival, events that may play a significant role in the acquisition of resistance to targeted therapies.

  7. 3D Computations and Experiments

    SciTech Connect

    Couch, R; Faux, D; Goto, D; Nikkel, D

    2003-05-12

    This project is in its first full year after the combining of two previously funded projects: ''3D Code Development'' and ''Dynamic Material Properties''. The motivation behind this move was to emphasize and strengthen the ties between the experimental work and the computational model development in the materials area. The next year's activities will indicate the merging of the two efforts. The current activity is structured in two tasks. Task A, ''Simulations and Measurements'', combines all the material model development and associated numerical work with the materials-oriented experimental activities. Task B, ''ALE3D Development'', is a continuation of the non-materials related activities from the previous project.

  8. Monitoring synaptic and neuronal activity in 3D with synthetic and genetic indicators using a compact acousto-optic lens two-photon microscope☆

    PubMed Central

    Fernández-Alfonso, Tomás; Nadella, K.M. Naga Srinivas; Iacaruso, M. Florencia; Pichler, Bruno; Roš, Hana; Kirkby, Paul A.; Silver, R. Angus

    2014-01-01

    Background Two-photon microscopy is widely used to study brain function, but conventional microscopes are too slow to capture the timing of neuronal signalling and imaging is restricted to one plane. Recent development of acousto-optic-deflector-based random access functional imaging has improved the temporal resolution, but the utility of these technologies for mapping 3D synaptic activity patterns and their performance at the excitation wavelengths required to image genetically encoded indicators have not been investigated. New method Here, we have used a compact acousto-optic lens (AOL) two-photon microscope to make high speed [Ca2+] measurements from spines and dendrites distributed in 3D with different excitation wavelengths (800–920 nm). Results We show simultaneous monitoring of activity from many synaptic inputs distributed over the 3D arborisation of a neuronal dendrite using both synthetic as well as genetically encoded indicators. We confirm the utility of AOL-based imaging for fast in vivo recordings by measuring, simultaneously, visually evoked responses in 100 neurons distributed over a 150 μm focal depth range. Moreover, we explore ways to improve the measurement of timing of neuronal activation by choosing specific regions within the cell soma. Comparison with existing methods These results establish that AOL-based 3D random access two-photon microscopy has a wider range of neuroscience applications than previously shown. Conclusions Our findings show that the compact AOL microscope design has the speed, spatial resolution, sensitivity and wavelength flexibility to measure 3D patterns of synaptic and neuronal activity on individual trials. PMID:24200507

  9. 3D modeling of effects of increased oxygenation and activity concentration in tumors treated with radionuclides and antiangiogenic drugs

    SciTech Connect

    Lagerloef, Jakob H.; Kindblom, Jon; Bernhardt, Peter

    2011-08-15

    Purpose: Formation of new blood vessels (angiogenesis) in response to hypoxia is a fundamental event in the process of tumor growth and metastatic dissemination. However, abnormalities in tumor neovasculature often induce increased interstitial pressure (IP) and further reduce oxygenation (pO{sub 2}) of tumor cells. In radiotherapy, well-oxygenated tumors favor treatment. Antiangiogenic drugs may lower IP in the tumor, improving perfusion, pO{sub 2} and drug uptake, by reducing the number of malfunctioning vessels in the tissue. This study aims to create a model for quantifying the effects of altered pO{sub 2}-distribution due to antiangiogenic treatment in combination with radionuclide therapy. Methods: Based on experimental data, describing the effects of antiangiogenic agents on oxygenation of GlioblastomaMultiforme (GBM), a single cell based 3D model, including 10{sup 10} tumor cells, was developed, showing how radionuclide therapy response improves as tumor oxygenation approaches normal tissue levels. The nuclides studied were {sup 90}Y, {sup 131}I, {sup 177}Lu, and {sup 211}At. The absorbed dose levels required for a tumor control probability (TCP) of 0.990 are compared for three different log-normal pO{sub 2}-distributions: {mu}{sub 1} = 2.483, {sigma}{sub 1} = 0.711; {mu}{sub 2} = 2.946, {sigma}{sub 2} = 0.689; {mu}{sub 3} = 3.689, and {sigma}{sub 3} = 0.330. The normal tissue absorbed doses will, in turn, depend on this. These distributions were chosen to represent the expected oxygen levels in an untreated hypoxic tumor, a hypoxic tumor treated with an anti-VEGF agent, and in normal, fully-oxygenated tissue, respectively. The former two are fitted to experimental data. The geometric oxygen distributions are simulated using two different patterns: one Monte Carlo based and one radially increasing, while keeping the log-normal volumetric distributions intact. Oxygen and activity are distributed, according to the same pattern. Results: As tumor pO{sub 2

  10. Discovery of Benzotriazolo[4,3-d][1,4]diazepines as Orally Active Inhibitors of BET Bromodomains.

    PubMed

    Taylor, Alexander M; Vaswani, Rishi G; Gehling, Victor S; Hewitt, Michael C; Leblanc, Yves; Audia, James E; Bellon, Steve; Cummings, Richard T; Côté, Alexandre; Harmange, Jean-Christophe; Jayaram, Hari; Joshi, Shivangi; Lora, Jose M; Mertz, Jennifer A; Neiss, Adrianne; Pardo, Eneida; Nasveschuk, Christopher G; Poy, Florence; Sandy, Peter; Setser, Jeremy W; Sims, Robert J; Tang, Yong; Albrecht, Brian K

    2016-02-11

    Inhibition of the bromodomains of the BET family, of which BRD4 is a member, has been shown to decrease myc and interleukin (IL) 6 in vivo, markers that are of therapeutic relevance to cancer and inflammatory disease, respectively. Herein we report substituted benzo[b]isoxazolo[4,5-d]azepines and benzotriazolo[4,3-d][1,4]diazepines as fragment-derived novel inhibitors of the bromodomain of BRD4. Compounds from these series were potent and selective in cells, and subsequent optimization of microsomal stability yielded representatives that demonstrated dose- and time-dependent reduction of plasma IL-6 in mice. PMID:26985289

  11. 75 FR 66802 - Calvert Cliffs Nuclear Power Plant, LLC; Calvert Cliffs Nuclear Power Plant, Unit Nos. 1 and 2...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-29

    ... COMMISSION Calvert Cliffs Nuclear Power Plant, LLC; Calvert Cliffs Nuclear Power Plant, Unit Nos. 1 and 2... Regulatory Commission (the Commission) has granted the request of Calvert Cliffs Nuclear Power Plant, LLC... Operating License Nos. DPR-53 and DPR-69 for the Calvert Cliffs Nuclear Power Plant, Unit Nos. 1 and...

  12. Natural hazards: Cracking cliffs feel the heat

    NASA Astrophysics Data System (ADS)

    Gischig, Valentin S.

    2016-05-01

    Rockfall often seems to occur spontaneously without an obvious cause. Monitoring of a granitic cliff reveals that cyclical temperature variations can subtly act to slowly and incrementally damage hard rock until failure is inevitable.

  13. Dynamics of a cliff top dune

    NASA Astrophysics Data System (ADS)

    Rasmussen, K. R.

    2012-12-01

    Morphological changes during more than 100 years have been investigated for a cliff-top dune complex at Rubjerg at the Danish North Sea coast. Here the lower 50 m of the cliff front is composed of Pleistocene steeply inclined floes of silt and clay with coarse sand in between which gives it a saw-tooth appearance. On top of this the dunes are found for several kilometres along the coastline. Due to erosion by the North Sea the cliff has retreated about 120 m between approximately 1880 and 1970 as indicated from two national surveys, and recent GPS-surveys indicate that erosion is continuing at a similar rate. Nevertheless the cliff top dune complex has survived, but its morphology has undergone some changed. The old maps indicate that around 1880 the dune complex was composed of several up to about 20 m high dunes streamlined in the East-West direction which is parallel to the prevailing wind direction. When protective planting started during the first half of the 20th Century the cliff top dunes gradually merged together forming a narrow, tall ridge parallel to the shore line with the highest part reaching about 90 m near 1970. In 1993 the highest points along the ridge was almost 95 m high, but then the protective planting was considerably reduced and recent annual GPS-surveys indicate that the dunes respond quickly to this by changing their morphology towards the original appearance. It is remarkable that despite the mass wasting caused by the constant erosion of the cliff front the dunes have remained more or less intact. Theoretical studies of hill flow indicate given the proper geometry of the cliff then suspension of even coarse grains can be a very effective agent for carrying sand from the exposed parts of the cliff front to and beyond the cliff-top. Mostly the sand grains are deposited within some hundred meters downwind of the cliff dune while silt is often carried more than 10 km inland. Field observations indicate that where the dislodged floes and

  14. 3D Computations and Experiments

    SciTech Connect

    Couch, R; Faux, D; Goto, D; Nikkel, D

    2004-04-05

    This project consists of two activities. Task A, Simulations and Measurements, combines all the material model development and associated numerical work with the materials-oriented experimental activities. The goal of this effort is to provide an improved understanding of dynamic material properties and to provide accurate numerical representations of those properties for use in analysis codes. Task B, ALE3D Development, involves general development activities in the ALE3D code with the focus of improving simulation capabilities for problems of mutual interest to DoD and DOE. Emphasis is on problems involving multi-phase flow, blast loading of structures and system safety/vulnerability studies.

  15. Development of the dynamic motion simulator of 3D micro-gravity with a combined passive/active suspension system

    NASA Technical Reports Server (NTRS)

    Yoshida, Kazuya; Hirose, Shigeo; Ogawa, Tadashi

    1994-01-01

    The establishment of those in-orbit operations like 'Rendez-Vous/Docking' and 'Manipulator Berthing' with the assistance of robotics or autonomous control technology, is essential for the near future space programs. In order to study the control methods, develop the flight models, and verify how the system works, we need a tool or a testbed which enables us to simulate mechanically the micro-gravity environment. There have been many attempts to develop the micro-gravity testbeds, but once the simulation goes into the docking and berthing operation that involves mechanical contacts among multi bodies, the requirement becomes critical. A group at the Tokyo Institute of Technology has proposed a method that can simulate the 3D micro-gravity producing a smooth response to the impact phenomena with relatively simple apparatus. Recently the group carried out basic experiments successfully using a prototype hardware model of the testbed. This paper will present our idea of the 3D micro-gravity simulator and report the results of our initial experiments.

  16. Coronal and transition-region Doppler shifts of an active region 3D-MHD model as indicator for the magnetic activity cycle of solar-like stars

    NASA Astrophysics Data System (ADS)

    Bourdin, Philippe A.

    2015-08-01

    For the Sun and solar-like stars, Doppler blueshifts are observed in the hot corona, while in average redshifts are seen in the cooler transition region layer below the corona. This clearly contradicts the idea of a continuous flow-equilibrium starting from a star's atmosphere and forming the stellar wind. To explain this, we implement a 3D-MHD model of the solar corona above an observed active region and use an atomic database to obtain the emission from the million Kelvin hot plasma. The generated EUV-bright loops system from the model compares well to the observed coronal loops. Therefore, we have access to realistic plasma parameters, including the flow dynamics within the active region core, and can derive total spectra as if we look the Sun as a star. We compare the model spectra to actual statistical observations of the Sun taken at different magnetic activity levels. We find characteristic Doppler-shift statistics that can be used to identify the magnetic activity state of the Sun and solar-like stars. This should help to model the variability of such stars by inferring their activity level from total spectra of coronal and transition-region emission lines.

  17. 3D Imaging.

    ERIC Educational Resources Information Center

    Hastings, S. K.

    2002-01-01

    Discusses 3 D imaging as it relates to digital representations in virtual library collections. Highlights include X-ray computed tomography (X-ray CT); the National Science Foundation (NSF) Digital Library Initiatives; output peripherals; image retrieval systems, including metadata; and applications of 3 D imaging for libraries and museums. (LRW)

  18. Mitigation of Alfvén activity in a tokamak by externally applied static 3D fields.

    PubMed

    Bortolon, A; Heidbrink, W W; Kramer, G J; Park, J-K; Fredrickson, E D; Lore, J D; Podestà, M

    2013-06-28

    The application of static magnetic field perturbations to a tokamak plasma is observed to alter the dynamics of high-frequency bursting Alfvén modes that are driven unstable by energetic ions. In response to perturbations with an amplitude of δB/B∼0.01 at the plasma boundary, the mode amplitude is reduced, the bursting frequency is increased, and the frequency chirp is smaller. For modes of weaker bursting character, the magnetic perturbation induces a temporary transition to a saturated continuous mode. Calculations of the perturbed distribution function indicate that the 3D perturbation affects the orbits of fast ions that resonate with the bursting modes. The experimental evidence represents an important demonstration of the possibility of controlling fast-ion instabilities through "phase-space engineering" of the fast-ion distribution function, by means of externally applied perturbation fields. PMID:23848889

  19. Development of 3D multimedia with advanced computer animation tools for outreach activities related to Meteor Science and Meteoritics

    NASA Astrophysics Data System (ADS)

    Madiedo, J. M.

    2012-09-01

    Documentaries related to Astronomy and Planetary Sciences are a common and very attractive way to promote the interest of the public in these areas. These educational tools can get benefit from new advanced computer animation software and 3D technologies, as these allow making these documentaries even more attractive. However, special care must be taken in order to guarantee that the information contained in them is serious and objective. In this sense, an additional value is given when the footage is produced by the own researchers. With this aim, a new documentary produced and directed by Prof. Madiedo has been developed. The documentary, which has been entirely developed by means of advanced computer animation tools, is dedicated to several aspects of Meteor Science and Meteoritics. The main features of this outreach and education initiative are exposed here.

  20. Laser gated viewing at ISL for vision through smoke, active polarimetry, and 3D imaging in NIR and SWIR wavelength bands

    NASA Astrophysics Data System (ADS)

    Laurenzis, Martin; Christnacher, Frank

    2013-12-01

    In this article, we want to give a review on the application of laser gated viewing for the improvement of vision cross-diffusing obstacles (smoke, turbid medium, …), the capturing of 3D scene information, or the study of material properties by polarimetric analysis at near-infrared (NIR) and shortwave-infrared (SWIR) wavelengths. Laser gated viewing has been studied since the 1960s as an active night vision method. Owing to enormous improvements in the development of compact and highly efficient laser sources and in the development of modern sensor technologies, the maturity of demonstrator systems rose during the past decades. Further, it was demonstrated that laser gated viewing has versatile sensing capabilities with application for long-range observation under certain degraded weather conditions, vision through obstacles and fog, active polarimetry, and 3D imaging.

  1. Combined 3D-QSAR, molecular docking and molecular dynamics study on thyroid hormone activity of hydroxylated polybrominated diphenyl ethers to thyroid receptors β

    SciTech Connect

    Li, Xiaolin; Ye, Li; Wang, Xiaoxiang; Wang, Xinzhou; Liu, Hongling; Zhu, Yongliang; Yu, Hongxia

    2012-12-15

    Several recent reports suggested that hydroxylated polybrominated diphenyl ethers (HO-PBDEs) may disturb thyroid hormone homeostasis. To illuminate the structural features for thyroid hormone activity of HO-PBDEs and the binding mode between HO-PBDEs and thyroid hormone receptor (TR), the hormone activity of a series of HO-PBDEs to thyroid receptors β was studied based on the combination of 3D-QSAR, molecular docking, and molecular dynamics (MD) methods. The ligand- and receptor-based 3D-QSAR models were obtained using Comparative Molecular Similarity Index Analysis (CoMSIA) method. The optimum CoMSIA model with region focusing yielded satisfactory statistical results: leave-one-out cross-validation correlation coefficient (q{sup 2}) was 0.571 and non-cross-validation correlation coefficient (r{sup 2}) was 0.951. Furthermore, the results of internal validation such as bootstrapping, leave-many-out cross-validation, and progressive scrambling as well as external validation indicated the rationality and good predictive ability of the best model. In addition, molecular docking elucidated the conformations of compounds and key amino acid residues at the docking pocket, MD simulation further determined the binding process and validated the rationality of docking results. -- Highlights: ► The thyroid hormone activities of HO-PBDEs were studied by 3D-QSAR. ► The binding modes between HO-PBDEs and TRβ were explored. ► 3D-QSAR, molecular docking, and molecular dynamics (MD) methods were performed.

  2. Analyses of Magnetic Structures of Active Region 11117 Evolution using a 3D Data-Driven Magnetohydrodynamic Model

    NASA Astrophysics Data System (ADS)

    Wu, Shi; Jiang, Chaowei; Feng, Xueshang

    We use the photospheric vector magnetograms obtained by Helioseismic and Magnetic Image (HMI) on-board the Solar Dynamic Observatory (SDO) as the boundary conditions for a Data-Driven CESE-MHD model (Jiang et al. 2012) to investigate the physical characteristics and evolution of magnetic field configurations in the corona before and after a solar eruptive event. Specifically, the evolution of AR11117 characteristics such as length of magnetic shear along the neutral line, current helicity, magnetic free energy and the energy flux across the photosphere due to flux emergence and surface flow are presented. The computed 3D magnetic field configuration are compared with AIA (Atmosphere Image Assembly) which shows remarkable resemblance. A topological analyses reveals that the small flare is correlated with a bald patch (BP, where the magnetic field is tangent to the photosphere), suggesting that the energy release of the flare is caused by magnetic reconnection associated with the BP separatrices. The total magnetic flux and energy keep increasing slightly in spite of flare, while the computed magnetic free energy drops during the flare by 10 (30) ergs which is adequate in providing the energy budget of a minor C-class confined flare as observed. Jiang, Chaowei, Xueshang, Feng, S. T Wu and Qiang Hu, Ap. J., 759:85, 2012 Nov 10

  3. TRACE 3-D documentation

    SciTech Connect

    Crandall, K.R.

    1987-08-01

    TRACE 3-D is an interactive beam-dynamics program that calculates the envelopes of a bunched beam, including linear space-charge forces, through a user-defined transport system. TRACE 3-D provides an immediate graphics display of the envelopes and the phase-space ellipses and allows nine types of beam-matching options. This report describes the beam-dynamics calculations and gives detailed instruction for using the code. Several examples are described in detail.

  4. Both cis and trans Activities of Foot-and-Mouth Disease Virus 3D Polymerase Are Essential for Viral RNA Replication

    PubMed Central

    Herod, Morgan R.; Ferrer-Orta, Cristina; Loundras, Eleni-Anna; Ward, Joseph C.; Verdaguer, Nuria; Rowlands, David J.

    2016-01-01

    ABSTRACT The Picornaviridae is a large family of positive-sense RNA viruses that contains numerous human and animal pathogens, including foot-and-mouth disease virus (FMDV). The picornavirus replication complex comprises a coordinated network of protein-protein and protein-RNA interactions involving multiple viral and host-cellular factors. Many of the proteins within the complex possess multiple roles in viral RNA replication, some of which can be provided in trans (i.e., via expression from a separate RNA molecule), while others are required in cis (i.e., expressed from the template RNA molecule). In vitro studies have suggested that multiple copies of the RNA-dependent RNA polymerase (RdRp) 3D are involved in the viral replication complex. However, it is not clear whether all these molecules are catalytically active or what other function(s) they provide. In this study, we aimed to distinguish between catalytically active 3D molecules and those that build a replication complex. We report a novel nonenzymatic cis-acting function of 3D that is essential for viral-genome replication. Using an FMDV replicon in complementation experiments, our data demonstrate that this cis-acting role of 3D is distinct from the catalytic activity, which is predominantly trans acting. Immunofluorescence studies suggest that both cis- and trans-acting 3D molecules localize to the same cellular compartment. However, our genetic and structural data suggest that 3D interacts in cis with RNA stem-loops that are essential for viral RNA replication. This study identifies a previously undescribed aspect of picornavirus replication complex structure-function and an important methodology for probing such interactions further. IMPORTANCE Foot-and-mouth disease virus (FMDV) is an important animal pathogen responsible for foot-and-mouth disease. The disease is endemic in many parts of the world with outbreaks within livestock resulting in major economic losses. Propagation of the viral genome

  5. Enhanced Metabolizing Activity of Human ES Cell-Derived Hepatocytes Using a 3D Culture System with Repeated Exposures to Xenobiotics.

    PubMed

    Kim, Jong Hyun; Jang, Yu Jin; An, Su Yeon; Son, Jeongsang; Lee, Jaehun; Lee, Gyunggyu; Park, Ji Young; Park, Han-Jin; Hwang, Dong-Youn; Kim, Jong-Hoon; Han, Jiyou

    2015-09-01

    Highly homogeneous and functional stem cell-derived hepatocyte-like cells (HLCs) are considered a promising option in the cell-based therapy of liver disease and the development of effective in vitro toxicity screening tools. However, the purity of cells and expression and/or activity of drug metabolizing enzymes in stem cell-derived HLCs are usually too low to be useful for clinical or in vitro applications. Here, we describe a highly optimized hepatic differentiation protocol, which produces >90% (BGO1 and CHA15) albumin-positive HLCs with no purification process from human embryonic stem cell lines. In addition, we show that hepatic enzyme gene expressions and activities were significantly improved by generating 3D spheroidal aggregate of HLCs, compared with 2D HLCs. The 3D differentiation method increased expression of nuclear receptors (NRs) that regulate the proper expression of key hepatic enzymes. Furthermore, significantly increased hepatic functions such as albumin and urea secretion were observed in 3D hepatic spheroids, compared with 2D HLCs. HLCs in the spheroid exhibited morphological and ultrastructural features of normal hepatocytes. Importantly, we show that repeated exposures to xenobiotics facilitated further functional maturation of HLC, as confirmed by increased expression of genes for drug metabolizing enzymes and transcription factors. In conclusion, the 3D culture system with repeated exposures to xenobiotics may be a new strategy for enhancing hepatic metabolizing ability of stem cell-derived HLCs as a cell source for in vitro high-throughput hepatotoxicity models. PMID:26089346

  6. Synthesis and antiviral activity of certain 4- and 4,5-disubstituted 7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3-d]pyrimidines.

    PubMed

    Pudlo, J S; Saxena, N K; Nassiri, M R; Turk, S R; Drach, J C; Townsend, L B

    1988-11-01

    In vitro evaluation of a series of previously prepared tubercidin analogues revealed that certain 5-halogen-substituted analogues were active against human cytomegalovirus (HCMV) at concentrations lower than those that produced comparable cytotoxicity in uninfected cells. In contrast, tubercidin was cytotoxic at all antiviral concentrations. Even though the antiviral selectivity of the 5-substituted compounds was slight, this observation led us to prepare a series of acyclic analogues. Treatment of the sodium salt of 4-chloropyrrolo[2,3-d]pyrimidine (2) with (2-acetoxyethoxy)methyl bromide (2a) provided the acyclic nucleoside 4-chloro-7-[(2-acetoxyethoxy)methyl]pyrrolo[2,3-d]pyrimidine (3). A nucleophilic displacement of the 4-chloro group with methoxide, methylamine, and dimethylamine yielded the corresponding 4-substituted compounds 4, 5, and 6, respectively, in good yield. Electrophilic substitution (chlorination, bromination, and iodination) was effected at the C-5 position of compound 3 with N-chlorosuccinimide, N-bromosuccinimide, and iodine monochloride, respectively, in methylene chloride. Removal of the acetyl group from these intermediates (7a-9a) with methanolic ammonia at room temperature afforded the 5-chloro (7b), 5-bromo (8b), and 5-iodo (9b) derivatives of 4-chloro-7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3-d]pyrimidine. Treatment of compounds 7b-9b with methanolic ammonia at an elevated temperature produced the corresponding 5-halotubercidin analogues 10, 11, and 12, respectively. An alternate procedure for the preparation of these 4,5-disubstituted 7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3-d]pyrimidines involved an electrophilic substitution prior to the condensation of the heterocycle with 2a. Treatment of 2 with N-chlorosuccinimide and N-bromosuccinimide gave compounds 13a and 13b, respectively. The condensation of 13a and 13b with 2a and subsequent treatment with methylamine and ethylamine furnished the corresponding 5-halo-4-substituted-pyrrolo[2,3-d

  7. Europa Ice Cliffs-High Resolution

    NASA Technical Reports Server (NTRS)

    1998-01-01

    This view of the Conamara Chaos region on Jupiter's moon Europa shows cliffs along the edges of high-standing ice plates. The washboard texture of the older terrain has been broken into plates which are separated by material with a jumbled texture. The cliffs themselves are rough and broadly scalloped, and smooth debris shed from the cliff faces is piled along the base. For scale, the height of the cliffs and size of the scalloped indentations are comparable to the famous cliff face of Mount Rushmore in South Dakota.

    This image was taken on December 16, 1997 at a range of 900 kilometers (540 miles) by the solid state imaging system (camera) on NASA's Galileo spacecraft. North is to the top right of the picture, and the sun illuminates the surface from the east. This image, centered at approximately 8 degrees north latitude and 273 degrees west longitude, covers an area approximately 1.5 kilometers by 4 kilometers (0.9 miles by 2.4 miles). The resolution is 9 meters (30 feet) per picture element.

    The Jet Propulsion Laboratory, Pasadena, CA manages the Galileo mission for NASA's Office of Space Science, Washington, DC. JPL is an operating division of California Institute of Technology (Caltech).

    This image and other images and data received from Galileo are posted on the World Wide Web, on the Galileo mission home page at URL http://www.jpl.nasa.gov/ galileo.

  8. Synthesis and anticancer activity of novel 4-morpholino-7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidine derivatives bearing chromone moiety.

    PubMed

    Sun, Chengyu; Chen, Chen; Xu, Shan; Wang, Jianqiang; Zhu, Yan; Kong, Dejia; Tao, Hong; Jin, Mengjia; Zheng, Pengwu; Zhu, Wufu

    2016-08-15

    Herein, we designed and synthesized of a novel series of 7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidine derivatives bearing chromone moiety (10a-j, 13a-j). All the compounds were evaluated for the IC50 values against five cancer cell lines (A549, PC-3, MCF-7, Hela and HepG2). Seven of the target compounds exhibited moderate to excellent cytotoxicity. For these compounds, we tested their inhibitory activities against mTOR kinase, and four of them were tested their inhibitory activities against PI3Kα kinase in further. The results indicated that the optimized compound 10j showed excellent inhibitory activity and cytotoxicity against mTOR kinase, PI3Kα kinase and five cancer cell lines with IC50 values of 1.1μM, 0.92μM and 8.77-14.3μM. Structure-activity relationships (SARs) and docking studies indicated that the thiopyrano[4,3-d]pyrimidine scaffolds exerted little effect on antitumor activities of target compounds. Substitutions of chromone moiety at C-6 position with carboxyl were benefit to the antitumor activities. PMID:27353887

  9. Changes in dissolved iron deposition to the oceans driven by human activity: a 3-D global modelling study

    NASA Astrophysics Data System (ADS)

    Myriokefalitakis, S.; Daskalakis, N.; Mihalopoulos, N.; Baker, A. R.; Nenes, A.; Kanakidou, M.

    2015-03-01

    The global atmospheric iron (Fe) cycle is parameterized in the global 3-D chemical transport model TM4-ECPL to simulate the proton- and the organic ligand-promoted mineral Fe dissolution as well as the aqueous-phase photochemical reactions between the oxidative states of Fe(III/II). Primary emissions of total (TFe) and dissolved (DFe) Fe associated with dust and combustion processes are also taken into account. TFe emissions are calculated to amount to ~35 Tg Fe yr-1. The model reasonably simulates the available Fe observations, supporting the reliability of the results of this study. Accounting for proton- and organic ligand-promoted Fe-dissolution in present-day TM4-ECPL simulations, the total Fe-dissolution is calculated to be ~0.163 Tg Fe yr-1 that accounts for up to ~50% of the calculated total DFe emissions. The atmospheric burden of DFe is calculated to be ~0.012 Tg Fe. DFe deposition presents strong spatial and temporal variability with an annual deposition flux ~0.489 Tg Fe yr-1 from which about 25% (~0.124 Tg Fe yr-1) are deposited over the ocean. The impact of air-quality on Fe deposition is studied by performing sensitivity simulations using preindustrial (year 1850), present (year 2008) and future (year 2100) emission scenarios. These simulations indicate that an increase (~2 times) in Fe-dissolution may have occurred in the past 150 years due to increasing anthropogenic emissions and thus atmospheric acidity. On the opposite, a decrease (~2 times) of Fe-dissolution is projected for near future, since atmospheric acidity is expected to be lower than present-day due to air-quality regulations of anthropogenic emissions. The organic ligand contribution to Fe dissolution shows inverse relationship to the atmospheric acidity thus its importance has decreased since the preindustrial period but is projected to increase in the future. The calculated changes also show that the atmospheric DFe supply to High-Nutrient-Low-Chlorophyll oceanic areas (HNLC

  10. 3D-Stereoscopic Analysis of Solar Active Region Loops: I: SoHo/EIT Observations at Temperatures of 1.0-1.5 MK

    NASA Technical Reports Server (NTRS)

    Aschwanden, Markus J.; Newmark, Jeff; Delaboudiniere, Jean-Pierre; Neupert, Werner M.; Portier-Fozzani, Fabrice; Gary, G. Allen; Zucker, Arik

    1998-01-01

    The three-dimensional (3D) structure of solar active region NOAA 7986 observed on 1996 August 30 with the Extrem-ultraviolet Imaging Telescope (EIT) onboard the Solar and Heliospheric Observatory (SoHO) is analyzed. We develop a new method of Dynamic Stereoscopy to reconstruct the 3D geometry of dynamically changing loops, which allows us to determine the orientation of the loop plane with respect to the line-of-sight, a prerequisite to correct properly for projection effects in 3D loop models. With this method and the filter-ratio technique applied to EIT 171 A and 195 A images we determine the 3D coordinates (x(s), y(s), z(s)), the loop width) w(s), the electron density n(sub e)(s), and the electron temperature T(sub e)(s) as function of the loop length s for 30 loop segments. Fitting the loop densities with an exponential density model n(sub e)(h) we find that the so inferred scale height temperatures, T(sub e)(sup lambda) = 1.22 +/- 0.23 MK, match closely the EIT filter-ratio temperatures, T(sub e)(sup FIT) = 1.21 +/- 0.06 MK. We conclude that these rather large-scale loops (with heights of h approx. equals 50 - 200 Mm) that dominate EIT 171 A images are close to thermal equilibrium. Most of the loops show no significant thickness variation w(s), but many exhibit a trend of increasing temperature (dT/ds greater than 0) above the footpoint.

  11. The Cliffs of Time. Cliffs of the Neuse State Park. An Environmental Education Learning Experience, Designed for Grades 6-8.

    ERIC Educational Resources Information Center

    O'Neal, Adrian

    This activity packet provides educators with a series of hands-on interdisciplinary classroom and outdoor education activities for grades 6-8 that focus on geology and prehistoric life at Cliffs of the Neuse State Park, North Carolina. The packet was designed to meet established curriculum objectives of the North Carolina Department of Public…

  12. Radiochromic 3D Detectors

    NASA Astrophysics Data System (ADS)

    Oldham, Mark

    2015-01-01

    Radiochromic materials exhibit a colour change when exposed to ionising radiation. Radiochromic film has been used for clinical dosimetry for many years and increasingly so recently, as films of higher sensitivities have become available. The two principle advantages of radiochromic dosimetry include greater tissue equivalence (radiologically) and the lack of requirement for development of the colour change. In a radiochromic material, the colour change arises direct from ionising interactions affecting dye molecules, without requiring any latent chemical, optical or thermal development, with important implications for increased accuracy and convenience. It is only relatively recently however, that 3D radiochromic dosimetry has become possible. In this article we review recent developments and the current state-of-the-art of 3D radiochromic dosimetry, and the potential for a more comprehensive solution for the verification of complex radiation therapy treatments, and 3D dose measurement in general.

  13. Bootstrapping 3D fermions

    NASA Astrophysics Data System (ADS)

    Iliesiu, Luca; Kos, Filip; Poland, David; Pufu, Silviu S.; Simmons-Duffin, David; Yacoby, Ran

    2016-03-01

    We study the conformal bootstrap for a 4-point function of fermions < ψψψψ> in 3D. We first introduce an embedding formalism for 3D spinors and compute the conformal blocks appearing in fermion 4-point functions. Using these results, we find general bounds on the dimensions of operators appearing in the ψ × ψ OPE, and also on the central charge C T . We observe features in our bounds that coincide with scaling dimensions in the GrossNeveu models at large N . We also speculate that other features could coincide with a fermionic CFT containing no relevant scalar operators.

  14. Multi-temporal Terrestrial Laser Scanner monitoring of coastal instability processes at Coroglio cliff

    NASA Astrophysics Data System (ADS)

    Caputo, Teresa; Somma, Renato; Marino, Ermanno; Matano, Fabio; Troise, Claudia; De Natale, Giuseppe

    2016-04-01

    The Coroglio cliff is a morphological evolution of the caldera rim of Neapolitan Yellow Tuff (NYT) in Campi Flegrei caldera (CFc) with an elevation of 150 m a.s.l. and a length of about 200 m. The lithology consists of NYT, extremely lithified, overlaid by less lithified recent products of the Phlegrean volcanism., These materials are highly erodible and, due to proximity to the sea, the sea wave and wind actions cause very strong erosion process. In the recent years Terrestrial Laser Scanner (TLS) technique is used for environmental monitoring purposes through the creation of high resolution Digital Surface Model (DSM) and Digital Terrain Model (DTM). This method allows the reconstruction, by means of a dense cloud of points, of a 3D model for the entire investigated area. The scans need to be performed from different points of view in order to ensure a good coverage of the area, because a widespread problem is the occurrence of shaded areas. In our study we used a long-range laser scanner model RIEGL VZ1000®. Numerous surveys (April 2013, June 2014, February 2015) have been performed for monitoring coastal cliff morphological evolution. An additional survey was executed in March 2015, shortly after a landslide occurrence. To validate the multi-temporal monitoring of the laser scanner, a "quick" comparison of the acquired point clouds has been carried out using an algorithm cloud-to-cloud, in order to identify 3D changes. Then 2.5D raster images of the different scans has been performed in GIS environment, also in order to allow a map overlay of the produced thematic layer, both raster and vector data (geology, contour map, orthophoto, and so on). The comparison of multi-temporal data have evidenced interesting geomorphological processes on the cliff. It was observed a very intense (about 6 m) local moving back at the base of the cliff, mainly due to the sea wave action during storms, while in cliff sectors characterized by less compact lithologies widespread

  15. Changes in dissolved iron deposition to the oceans driven by human activity: a 3-D global modelling study

    NASA Astrophysics Data System (ADS)

    Myriokefalitakis, S.; Daskalakis, N.; Mihalopoulos, N.; Baker, A. R.; Nenes, A.; Kanakidou, M.

    2015-07-01

    The global atmospheric iron (Fe) cycle is parameterized in the global 3-D chemical transport model TM4-ECPL to simulate the proton- and the organic ligand-promoted mineral-Fe dissolution as well as the aqueous-phase photochemical reactions between the oxidative states of Fe (III/II). Primary emissions of total (TFe) and dissolved (DFe) Fe associated with dust and combustion processes are also taken into account, with TFe mineral emissions calculated to amount to ~ 35 Tg-Fe yr-1 and TFe emissions from combustion sources of ~ 2 Tg-Fe yr-1. The model reasonably simulates the available Fe observations, supporting the reliability of the results of this study. Proton- and organic ligand-promoted Fe dissolution in present-day TM4-ECPL simulations is calculated to be ~ 0.175 Tg-Fe yr-1, approximately half of the calculated total primary DFe emissions from mineral and combustion sources in the model (~ 0.322 Tg-Fe yr-1). The atmospheric burden of DFe is calculated to be ~ 0.024 Tg-Fe. DFe deposition presents strong spatial and temporal variability with an annual flux of ~ 0.496 Tg-Fe yr-1, from which about 40 % (~ 0.191 Tg-Fe yr-1) is deposited over the ocean. The impact of air quality on Fe deposition is studied by performing sensitivity simulations using preindustrial (year 1850), present (year 2008) and future (year 2100) emission scenarios. These simulations indicate that about a 3 times increase in Fe dissolution may have occurred in the past 150 years due to increasing anthropogenic emissions and thus atmospheric acidity. Air-quality regulations of anthropogenic emissions are projected to decrease atmospheric acidity in the near future, reducing to about half the dust-Fe dissolution relative to the present day. The organic ligand contribution to Fe dissolution shows an inverse relationship to the atmospheric acidity, thus its importance has decreased since the preindustrial period but is projected to increase in the future. The calculated changes also show that the

  16. Do Higher Sea-cliff Retreat Rates Imply Faster Sea-cliff Retreat?

    NASA Astrophysics Data System (ADS)

    Mushkin, A.; Katz, O.; Porat, N.

    2015-12-01

    Inland retreat of sea cliffs in response to post LGM (last glacial maximum) sea-level rise is an ongoing process that affects coastal environments and communities worldwide. Here, we examine a globally recurring pattern where reported sea-cliff retreat rates since the 20th century often appear to exceed longer-term millennial-scale ('background') rates that rarely exceed ~0.1 m/yr. Focusing on Israel's 30-km-long Mediterranean 'Sharon' sea-cliff as a case study we demonstrate that such apparent increase in rates may also reflect a widely acknowledged sampling bias in geologic rate estimates inferred from observation time windows ('Tobs') shorter than process episodicity. We show that this possible bias leads to an ambiguity in conventional rate estimates obtained by averaging observed retreat distances over Tobs, and that as a result despite ubiquitous and robust observations of cliff retreat since the 20th century (e.g., aerial photographs) recent/current retreat rates for many of the world's episodically retreating sea cliffs remain essentially unknown. To address this present limitation in our ability to detect and quantify recent changes in sea-cliff retreat rates we use airborne LiDAR to measure the continuous wave-driven volumetric erosion of collapsed material from the cliff base as an effective upper-bound constraint for the m/yr rate of episodic retreat of the cliff itself. We find that while conventional retreat rate estimates since the 20th century along the Sharon sea cliff artefactually increase up to several m/yr as an inverse function of Tobs, the LiDAR-constrained retreat rates are not susceptible to this sampling bias, are comparable to the cliff's background retreat rate of 0.03-0.07 m/yr since the mid Holocene and thus indicate no recent acceleration in retreat. This ability to unambiguously constrain sea-cliff retreat rates with annual to decadal-scale observations directly impacts the global-scale push to quantify, better understand and

  17. 3D nanospherical CdxZn1-xS/reduced graphene oxide composites with superior photocatalytic activity and photocorrosion resistance

    NASA Astrophysics Data System (ADS)

    Huang, Meina; Yu, Jianhua; Deng, Changshun; Huang, Yingheng; Fan, Minguang; Li, Bin; Tong, Zhangfa; Zhang, Feiyue; Dong, Lihui

    2016-03-01

    Herein, a series of CdxZn1-xS and sulfide/graphene photocatalysts with 3D nanospherical framework have been successfully fabricated by one-pot solvothermal method for the first time. The morphology and structure of samples were confirmed by X-ray diffraction (XRD), field-emission scanning electron microscopy (FESEM), high-resolution transmission electron microscopy (HRTEM), energy-dispersive X-ray (EDX) spectrometry, N2 adsorption, Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS) and ultraviolet-visible diffuse reflectance spectroscopy (UV-vis DRS). The as-prepared samples exhibit excellent photocatalytic activities and photocorrosion resistance in the degradation of dyes under visible light. The Cd0.5Zn0.5S/rGO sample shows the most efficient in the photodegradation of methyl orange (MO). It takes about 30 min for degradation completely. The enhanced photocatalytic activity is mainly attributed to the slow photon enhancement of the 3D structure, and the heterojunction between the 3D nanospherical Cd0.5Zn0.5S solid solutions and a high quality 2D rGO support, which can greatly promote the separation of light-induced electrons and holes. Moreover, the large SBET and extended light absorption range also play an important role for improving the photocatalytic activity. The high photocatalytic stability is due to the successful inhibition of the photocorrosion of Cd0.5Zn0.5S/rGO by forming heterojunction between CdS and ZnS, and transferring the photogenerated electrons of Cd0.5Zn0.5S to rGO. The present work can provide rational design of graphene-based photocatalysts with large contact interface and strong interaction between the composites for other application.

  18. A facile synthesis and fungicidal activities of 2-(alkylamino)-5,6-dimethylthieno[2,3-d]pyrimidin-4(3H)-ones

    PubMed Central

    Zheng, Ai-Hua; Ruan, Xu-Zhi

    2008-01-01

    Summary The aza-Wittig reactions of iminophosphorane 3 with aromatic isocyanates generated carbodiimides 4, which were reacted with alkylamines under mild conditions to give a series of 2-(alkylamino)-5,6-dimethylthieno[2,3-d]pyrimidin-4(3H)-ones 6 and 8 in satisfactory yield. Their structures were confirmed by 1H NMR, EI-MS, IR and elementary analysis, and compound 8c was further analyzed by single-crystal X-ray diffraction. The preliminary bioassays indicated that these compounds showed excellent fungicidal activities against six kinds of fungi. PMID:19190737

  19. Osseointegration of dental implants in 3D-printed synthetic onlay grafts customized according to bone metabolic activity in recipient site.

    PubMed

    Tamimi, Faleh; Torres, Jesus; Al-Abedalla, Khadijeh; Lopez-Cabarcos, Enrique; Alkhraisat, Mohammad H; Bassett, David C; Gbureck, Uwe; Barralet, Jake E

    2014-07-01

    Onlay grafts made of monolithic microporous monetite bioresorbable bioceramics have the capacity to conduct bone augmentation. However, there is heterogeneity in the graft behaviour in vivo that seems to correlate with the host anatomy. In this study, we sought to investigate the metabolic activity of the regenerated bone in monolithic monetite onlays by using positron emission tomography-computed tomography (PET-CT) in rats. This information was used to optimize the design of monetite onlays with different macroporous architecture that were then fabricated using a 3D-printing technique. In vivo, bone augmentation was attempted with these customized onlays in rabbits. PET-CT findings demonstrated that bone metabolism in the calvarial bone showed higher activity in the inferior and lateral areas of the onlays. Histological observations revealed higher bone volume (up to 47%), less heterogeneity and more implant osseointegration (up to 38%) in the augmented bone with the customized monetite onlays. Our results demonstrated for the first time that it is possible to achieve osseointegration of dental implants in bone augmented with 3D-printed synthetic onlays. It was also observed that designing the macropore geometry according to the bone metabolic activity was a key parameter in increasing the volume of bone augmented within monetite onlays. PMID:24726538

  20. Inhibition of Phosphodiesterase-4 (PDE4) activity triggers luminal apoptosis and AKT dephosphorylation in a 3-D colonic-crypt model

    PubMed Central

    2012-01-01

    Background We previously established a three-dimensional (3-D) colonic crypt model using HKe3 cells which are human colorectal cancer (CRC) HCT116 cells with a disruption in oncogenic KRAS, and revealed the crucial roles of oncogenic KRAS both in inhibition of apoptosis and in disruption of cell polarity; however, the molecular mechanism of KRAS-induced these 3-D specific biological changes remains to be elucidated. Results Among the genes that were upregulated by oncogenic KRAS in this model, we focused on the phosphodiesterase 4B (PDE4B) of which expression levels were found to be higher in clinical tumor samples from CRC patients in comparison to those from healthy control in the public datasets of gene expression analysis. PDE4B2 was specifically overexpressed among other PDE4 isoforms, and re-expression of oncogenic KRAS in HKe3 cells resulted in PDE4B overexpression. Furthermore, the inhibition of PDE4 catalytic activity using rolipram reverted the disorganization of HCT116 cells into the normal physiologic state of the epithelial cell polarity by inducing the apical assembly of ZO-1 (a tight junction marker) and E-cadherin (an adherens junction marker) and by increasing the activity of caspase-3 (an apoptosis marker) in luminal cavities. Notably, rolipram reduced the AKT phosphorylation, which is known to be associated with the disruption of luminal cavity formation and CRC development. Similar results were also obtained using PDE4B2-shRNAs. In addition, increased expression of PDE4B mRNA was found to be correlated with relapsed CRC in a public datasets of gene expression analysis. Conclusions These results collectively suggested that PDE4B is upregulated by oncogenic KRAS, and also that the inhibition of PDE4 catalytic activity can induce both epithelial cell polarity and luminal apoptosis in CRC, thus highlighting the utility of our 3-D culture (3 DC) model for the KRAS-induced development of CRC in 3-D microenvironment. Indeed, using this model, we

  1. A coupled 3D-1D numerical monodomain solver for cardiac electrical activation in the myocardium with detailed Purkinje network

    NASA Astrophysics Data System (ADS)

    Vergara, Christian; Lange, Matthias; Palamara, Simone; Lassila, Toni; Frangi, Alejandro F.; Quarteroni, Alfio

    2016-03-01

    We present a model for the electrophysiology in the heart to handle the electrical propagation through the Purkinje system and in the myocardium, with two-way coupling at the Purkinje-muscle junctions. In both the subproblems the monodomain model is considered, whereas at the junctions a resistor element is included that induces an orthodromic propagation delay from the Purkinje network towards the heart muscle. We prove a sufficient condition for convergence of a fixed-point iterative algorithm to the numerical solution of the coupled problem. Numerical comparison of activation patterns is made with two different combinations of models for the coupled Purkinje network/myocardium system, the eikonal/eikonal and the monodomain/monodomain models. Test cases are investigated for both physiological and pathological activation of a model left ventricle. Finally, we prove the reliability of the monodomain/monodomain coupling on a realistic scenario. Our results underlie the importance of using physiologically realistic Purkinje-trees with propagation solved using the monodomain model for simulating cardiac activation.

  2. Adaptive step ODE algorithms for the 3D simulation of electric heart activity with graphics processing units.

    PubMed

    Garcia-Molla, V M; Liberos, A; Vidal, A; Guillem, M S; Millet, J; Gonzalez, A; Martinez-Zaldivar, F J; Climent, A M

    2014-01-01

    In this paper we studied the implementation and performance of adaptive step methods for large systems of ordinary differential equations systems in graphics processing units, focusing on the simulation of three-dimensional electric cardiac activity. The Rush-Larsen method was applied in all the implemented solvers to improve efficiency. We compared the adaptive methods with the fixed step methods, and we found that the fixed step methods can be faster while the adaptive step methods are better in terms of accuracy and robustness. PMID:24377685

  3. 2012 Cliff Weiss Memorial Essay Contest Winners

    ERIC Educational Resources Information Center

    Techniques: Connecting Education and Careers (J3), 2012

    2012-01-01

    This article presents the winners of the 2012 Cliff Weiss Memorial Essay Contest. They are Naim Owens from Washington, DC, and Colissa Menke from Warrensburg, Missouri. The 2012 essay topic is "How do you feel CTE prepares individuals, including yourself, for a future career?"

  4. 3D-QSAR modelling dataset of bioflavonoids for predicting the potential modulatory effect on P-glycoprotein activity.

    PubMed

    Wongrattanakamon, Pathomwat; Lee, Vannajan Sanghiran; Nimmanpipug, Piyarat; Jiranusornkul, Supat

    2016-12-01

    The data is obtained from exploring the modulatory activities of bioflavonoids on P-glycoprotein function by ligand-based approaches. Multivariate Linear-QSAR models for predicting the induced/inhibitory activities of the flavonoids were created. Molecular descriptors were initially used as independent variables and a dependent variable was expressed as pFAR. The variables were then used in MLR analysis by stepwise regression calculation to build the linear QSAR data. The entire dataset consisted of 23 bioflavonoids was used as a training set. Regarding the obtained MLR QSAR model, R of 0.963, R (2)=0.927, [Formula: see text], SEE=0.197, F=33.849 and q (2)=0.927 were achieved. The true predictabilities of QSAR model were justified by evaluation with the external dataset (Table 4). The pFARs of representative flavonoids were predicted by MLR QSAR modelling. The data showed that internal and external validations may generate the same conclusion. PMID:27626051

  5. Effects of Long-Term Trampling on the Above-Ground Forest Vegetation and Soil Seed Bank at the Base of Limestone Cliffs

    NASA Astrophysics Data System (ADS)

    Rusterholz, Hans-Peter; Verhoustraeten, Christine; Baur, Bruno

    2011-11-01

    Exposed limestone cliffs in central Europe harbor a highly divers flora with many rare and endangered species. During the past few decades, there has been increasing recreational use of these cliffs, which has caused local environmental disturbances. Successful restoration strategies hinge on identifying critical limitations. We examined the composition of aboveground forest vegetation and density and species composition of seeds in the soil seed bank at the base of four limestone cliffs in mixed deciduous forests that are intensively disturbed by human trampling and at four undisturbed cliffs in the Jura Mountains in northwestern Switzerland. We found that long-term human trampling reduced total aboveground vegetation cover at the base of cliffs and caused a significant shift in the plant-species composition. Compared with undisturbed cliffs, total seed density was lower in disturbed cliffs. Human trampling also altered the species composition of seeds in the soil seed bank. Seeds of unintentionally introduced, stress-tolerant, and ruderal species dominated the soil seed bank at the base of disturbed cliffs. Our findings indicate that a restoration of degraded cliff bases from the existing soil seed bank would result in a substantial change of the original unique plant composition. Active seed transfer, or seed flux from adjacent undisturbed forest areas, is essential for restoration success.

  6. 76 FR 4391 - Calvert Cliffs Nuclear Power Plant, LLC, Calvert Cliffs Nuclear Power Plant, Unit Nos. 1 and 2...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-25

    ... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Calvert Cliffs Nuclear Power Plant, LLC, Calvert Cliffs Nuclear Power Plant, Unit Nos. 1 and 2; Exemption 1.0 Background Calvert Cliffs Nuclear Power Plant, LLC, the licensee, is the holder of Facility Operating License Nos. DPR-53 and DPR-69...

  7. 76 FR 39908 - Calvert Cliffs Nuclear Power Plant, LLC; Calvert Cliffs Nuclear Power Plant, Unit Nos. 1 and 2...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-07

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  8. 3D microscope

    NASA Astrophysics Data System (ADS)

    Iizuka, Keigo

    2008-02-01

    In order to circumvent the fact that only one observer can view the image from a stereoscopic microscope, an attachment was devised for displaying the 3D microscopic image on a large LCD monitor for viewing by multiple observers in real time. The principle of operation, design, fabrication, and performance are presented, along with tolerance measurements relating to the properties of the cellophane half-wave plate used in the design.

  9. Rheological variations across an active rift system -- results from lithosphere-scale 3D gravity and thermal models of the Kenya Rift

    NASA Astrophysics Data System (ADS)

    Meeßen, Christian; Sippel, Judith; Cacace, Mauro; Scheck-Wenderoth, Magdalena; Fishwick, Stewart; Heine, Christian; Strecker, Manfred R.

    2015-04-01

    Due to its tectono-volcanic activity and economic (geothermal and petroleum) potential, the eastern branch of the East African Rift System (EARS) is one of the best studied extensional systems worldwide and an important natural laboratory for the development of geodynamic concepts on rifting and nascent continental break-up. The Kenya Rift, an integral part of the eastern branch of the EARS, has formed in the area of weak Proterozoic crust of the Mozambique mobile belt adjacent to the rheologically stronger Archean Tanzania craton. To assess the variations in lithospheric strength between different tectonic domains and their influence on the tectonic evolution of the region, we developed a set of structural, density, thermal and rheological 3D models. For these models we integrated multi-disciplinary information, such as published geological field data, sediment thicknesses, well information, existing structural models, seismic refraction and reflection data, seismic tomography, gravity and heat-flow data. Our main approach focused on combined 3D isostatic and gravity modelling. The resulting lithosphere-scale 3D density model provides new insights into the depth distribution of the crust-mantle boundary and thickness variations of different crustal density domains. The latter further facilitate interpretations of variations of lithologies and related physical rock properties. By considering lithology-dependent heat production and thermal conductivity, we calculate the conductive thermal field across the region of the greater Kenya Rift. Finally, the assessed variations in lithology and temperature allow deriving differences in the integrated strength of the lithosphere across the different tectonic domains.

  10. Active shape models for a fully automated 3D segmentation of the liver--an evaluation on clinical data.

    PubMed

    Heimann, Tobias; Wolf, Ivo; Meinzer, Hans-Peter

    2006-01-01

    This paper presents an evaluation of the performance of a three-dimensional Active Shape Model (ASM) to segment the liver in 48 clinical CT scans. The employed shape model is built from 32 samples using an optimization approach based on the minimum description length (MDL). Three different gray-value appearance models (plain intensity, gradient and normalized gradient profiles) are created to guide the search. The employed segmentation techniques are ASM search with 10 and 30 modes of variation and a deformable model coupled to a shape model with 10 modes of variation. To assess the segmentation performance, the obtained results are compared to manual segmentations with four different measures (overlap, average distance, RMS distance and ratio of deviations larger 5mm). The only appearance model delivering usable results is the normalized gradient profile. The deformable model search achieves the best results, followed by the ASM search with 30 modes. Overall, statistical shape modeling delivers very promising results for a fully automated segmentation of the liver. PMID:17354754

  11. Graph-based active learning of agglomeration (GALA): a Python library to segment 2D and 3D neuroimages

    PubMed Central

    Nunez-Iglesias, Juan; Kennedy, Ryan; Plaza, Stephen M.; Chakraborty, Anirban; Katz, William T.

    2014-01-01

    The aim in high-resolution connectomics is to reconstruct complete neuronal connectivity in a tissue. Currently, the only technology capable of resolving the smallest neuronal processes is electron microscopy (EM). Thus, a common approach to network reconstruction is to perform (error-prone) automatic segmentation of EM images, followed by manual proofreading by experts to fix errors. We have developed an algorithm and software library to not only improve the accuracy of the initial automatic segmentation, but also point out the image coordinates where it is likely to have made errors. Our software, called gala (graph-based active learning of agglomeration), improves the state of the art in agglomerative image segmentation. It is implemented in Python and makes extensive use of the scientific Python stack (numpy, scipy, networkx, scikit-learn, scikit-image, and others). We present here the software architecture of the gala library, and discuss several designs that we consider would be generally useful for other segmentation packages. We also discuss the current limitations of the gala library and how we intend to address them. PMID:24772079

  12. Automated 3-D Segmentation of Lungs With Lung Cancer in CT Data Using a Novel Robust Active Shape Model Approach

    PubMed Central

    Sun, Shanhui; Bauer, Christian; Beichel, Reinhard

    2013-01-01

    Segmentation of lungs with (large) lung cancer regions is a nontrivial problem. We present a new fully automated approach for segmentation of lungs with such high-density pathologies. Our method consists of two main processing steps. First, a novel robust active shape model (RASM) matching method is utilized to roughly segment the outline of the lungs. The initial position of the RASM is found by means of a rib cage detection method. Second, an optimal surface finding approach is utilized to further adapt the initial segmentation result to the lung. Left and right lungs are segmented individually. An evaluation on 30 data sets with 40 abnormal (lung cancer) and 20 normal left/right lungs resulted in an average Dice coefficient of 0.975 ± 0.006 and a mean absolute surface distance error of 0.84 ± 0.23 mm, respectively. Experiments on the same 30 data sets showed that our methods delivered statistically significant better segmentation results, compared to two commercially available lung segmentation approaches. In addition, our RASM approach is generally applicable and suitable for large shape models. PMID:21997248

  13. 3D-Reconstruction of recent volcanic activity from ROV-video, Charles Darwin Seamounts, Cape Verdes

    NASA Astrophysics Data System (ADS)

    Kwasnitschka, T.; Hansteen, T. H.; Kutterolf, S.; Freundt, A.; Devey, C. W.

    2011-12-01

    As well as providing well-localized samples, Remotely Operated Vehicles (ROVs) produce huge quantities of visual data whose potential for geological data mining has seldom if ever been fully realized. We present a new workflow to derive essential results of field geology such as quantitative stratigraphy and tectonic surveying from ROV-based photo and video material. We demonstrate the procedure on the Charles Darwin Seamounts, a field of small hot spot volcanoes recently identified at a depth of ca. 3500m southwest of the island of Santo Antao in the Cape Verdes. The Charles Darwin Seamounts feature a wide spectrum of volcanic edifices with forms suggestive of scoria cones, lava domes, tuff rings and maar-type depressions, all of comparable dimensions. These forms, coupled with the highly fragmented volcaniclastic samples recovered by dredging, motivated surveying parts of some edifices down to centimeter scale. ROV-based surveys yielded volcaniclastic samples of key structures linked by extensive coverage of stereoscopic photographs and high-resolution video. Based upon the latter, we present our workflow to derive three-dimensional models of outcrops from a single-camera video sequence, allowing quantitative measurements of fault orientation, bedding structure, grain size distribution and photo mosaicking within a geo-referenced framework. With this information we can identify episodes of repetitive eruptive activity at individual volcanic centers and see changes in eruptive style over time, which, despite their proximity to each other, is highly variable.

  14. Geological 3D modeling for excavation activity in an underground marble quarry in the Apuan Alps (Italy)

    NASA Astrophysics Data System (ADS)

    Vanneschi, Claudio; Salvini, Riccardo; Massa, Giovanni; Riccucci, Silvia; Borsani, Angelo

    2014-08-01

    The three-dimensional laser scanning technique has recently become common in diverse working environments. Even in geology, where further development is needed, this technique is increasingly useful in tackling various problems such as stability investigations or geological and geotechnical monitoring. Three-dimensional laser scanning supplies detailed and complete geometrical information in short working times, as a result of the acquisition of a large number of data-points that accurately model the detected surfaces. Moreover, it is possible to combine these data with high quality photographic images so as to provide important information for geological applications, as follows. A working approach, that combines terrestrial laser scanning and traditional geological surveys, is presented. A three-dimensional model, that includes information about the geological structure in an underground quarry in the Apuan Alps, is realized. This procedure is adaptable to other geological contexts, and because of its operating speed and accuracy it is invaluable for optimal excavation, in which a proper planning of quarrying activity is vital for safety and commercial reasons.

  15. Investigation of hydrothermal activity at Campi Flegrei caldera using 3D simulations: extension to high temperature processes

    NASA Astrophysics Data System (ADS)

    Afanasyev, Andrey; Costa, Antonio; Chiodini, Giovanni

    2015-04-01

    Hydrothermal activity at Campi Flegrei caldera is simulated by using the multiphase code MUFITS (www.mufits.imec.msu.ru). We provide a brief description of the simulator covering the mathematical formulation and its applicability at elevated supercritical temperatures. Then we apply, for the first time, the code to hydrothermal systems investigating the Campi Flegrei caldera case. We consider both shallow subcritical regions and deep supercritical regions of the hydrothermal system. We impose sophisticated boundary conditions at the surface to provide a better description of the reservoir interactions with the atmosphere and the sea. Finally we carry out a parametric study and compare the simulation results with gas temperature and composition, gas and heat fluxes, and temperature measurements in the wells of that area. Results of the parametric study show that flow rate, composition, and temperature of the hot gas mixture injected at depth, and the initial geothermal gradient strongly control parameters monitored at Solfatara. Comparisons with observations show a very good match and suggest that the best guesses for the injected hot (~700 C) fluid mass flow rate is about 50-100 kg/s and the initial geothermal gradient is 120 C/km. Of particular interest resulted the comparison between the simulated thermal profiles and those measured in geothermal wells. Keeping in mind the uncertainties due to the heterogeneities of the system, the good match obtained for the wells in the eastern and north sectors of the caldera (located some km far from Solfatara) suggest that the model can reproduce the gross features of the Campi Flegrei hydrothermal system and implicitly support the hypothesis of a single (or major) deep source of magmatic fluid located close to the centre of the caldera. Surprising results were also obtained by comparing simulated and observed (Agnano well) temperature profiles in a zone close to the gas plume: in this case the simulations clearly suggested

  16. Artemisinin activity-based probes identify multiple molecular targets within the asexual stage of the malaria parasites Plasmodium falciparum 3D7

    PubMed Central

    Ismail, Hanafy M.; Barton, Victoria; Phanchana, Matthew; Charoensutthivarakul, Sitthivut; Wong, Michael H. L.; Hemingway, Janet; Biagini, Giancarlo A.; O’Neill, Paul M.; Ward, Stephen A.

    2016-01-01

    The artemisinin (ART)-based antimalarials have contributed significantly to reducing global malaria deaths over the past decade, but we still do not know how they kill parasites. To gain greater insight into the potential mechanisms of ART drug action, we developed a suite of ART activity-based protein profiling probes to identify parasite protein drug targets in situ. Probes were designed to retain biological activity and alkylate the molecular target(s) of Plasmodium falciparum 3D7 parasites in situ. Proteins tagged with the ART probe can then be isolated using click chemistry before identification by liquid chromatography–MS/MS. Using these probes, we define an ART proteome that shows alkylated targets in the glycolytic, hemoglobin degradation, antioxidant defense, and protein synthesis pathways, processes essential for parasite survival. This work reveals the pleiotropic nature of the biological functions targeted by this important class of antimalarial drugs. PMID:26858419

  17. Artemisinin activity-based probes identify multiple molecular targets within the asexual stage of the malaria parasites Plasmodium falciparum 3D7.

    PubMed

    Ismail, Hanafy M; Barton, Victoria; Phanchana, Matthew; Charoensutthivarakul, Sitthivut; Wong, Michael H L; Hemingway, Janet; Biagini, Giancarlo A; O'Neill, Paul M; Ward, Stephen A

    2016-02-23

    The artemisinin (ART)-based antimalarials have contributed significantly to reducing global malaria deaths over the past decade, but we still do not know how they kill parasites. To gain greater insight into the potential mechanisms of ART drug action, we developed a suite of ART activity-based protein profiling probes to identify parasite protein drug targets in situ. Probes were designed to retain biological activity and alkylate the molecular target(s) of Plasmodium falciparum 3D7 parasites in situ. Proteins tagged with the ART probe can then be isolated using click chemistry before identification by liquid chromatography-MS/MS. Using these probes, we define an ART proteome that shows alkylated targets in the glycolytic, hemoglobin degradation, antioxidant defense, and protein synthesis pathways, processes essential for parasite survival. This work reveals the pleiotropic nature of the biological functions targeted by this important class of antimalarial drugs. PMID:26858419

  18. Synthesis, Anticancer Activity, Effect on Cell Cycle Profile, and Apoptosis-Inducing Ability of Novel Hexahydrocyclooctathieno[2,3-d]pyrimidine Derivatives.

    PubMed

    Kassab, Asmaa Elsayed; Gedawy, Ehab Mohamed; El-Malah, Afaf Ali; Abdelghany, Tamer Mohamed; Abdel-Bakky, Mohamed Sadek

    2016-01-01

    A novel series of hexahydrocyclooctathieno[2,3-d]pyrimidines was synthesized. Investigation of the anticancer activity of these derivatives revealed that compounds 2a and b showed broad-spectrum anticancer activity in nanomolar to micromolar concentrations. In particular, compound 2b showed a concentration required for 50% inhibition of cell growth (GI50) value of less than 1 µM against 20 cancer cell lines. Compounds 2a and b induced G2/M- and S-phase cell cycle arrest in human colon adenocarcinoma (HCT116) and human breast adenocarcinoma (MCF7) cell lines with a concomitant increase in the pre-G cell population in a time-dependent manner. Furthermore, compound 2b increased the nuclear expression of the proapoptotic protein cleaved caspase-3, indicating that apoptosis has an important role, at least in part, in the cancer cell death induced by the new compounds. PMID:27150481

  19. Accurate and Fully Automatic Hippocampus Segmentation Using Subject-Specific 3D Optimal Local Maps Into a Hybrid Active Contour Model

    PubMed Central

    Gkontra, Polyxeni; Daras, Petros; Maglaveras, Nicos

    2014-01-01

    Assessing the structural integrity of the hippocampus (HC) is an essential step toward prevention, diagnosis, and follow-up of various brain disorders due to the implication of the structural changes of the HC in those disorders. In this respect, the development of automatic segmentation methods that can accurately, reliably, and reproducibly segment the HC has attracted considerable attention over the past decades. This paper presents an innovative 3-D fully automatic method to be used on top of the multiatlas concept for the HC segmentation. The method is based on a subject-specific set of 3-D optimal local maps (OLMs) that locally control the influence of each energy term of a hybrid active contour model (ACM). The complete set of the OLMs for a set of training images is defined simultaneously via an optimization scheme. At the same time, the optimal ACM parameters are also calculated. Therefore, heuristic parameter fine-tuning is not required. Training OLMs are subsequently combined, by applying an extended multiatlas concept, to produce the OLMs that are anatomically more suitable to the test image. The proposed algorithm was tested on three different and publicly available data sets. Its accuracy was compared with that of state-of-the-art methods demonstrating the efficacy and robustness of the proposed method. PMID:27170866

  20. Accurate and Fully Automatic Hippocampus Segmentation Using Subject-Specific 3D Optimal Local Maps Into a Hybrid Active Contour Model.

    PubMed

    Zarpalas, Dimitrios; Gkontra, Polyxeni; Daras, Petros; Maglaveras, Nicos

    2014-01-01

    Assessing the structural integrity of the hippocampus (HC) is an essential step toward prevention, diagnosis, and follow-up of various brain disorders due to the implication of the structural changes of the HC in those disorders. In this respect, the development of automatic segmentation methods that can accurately, reliably, and reproducibly segment the HC has attracted considerable attention over the past decades. This paper presents an innovative 3-D fully automatic method to be used on top of the multiatlas concept for the HC segmentation. The method is based on a subject-specific set of 3-D optimal local maps (OLMs) that locally control the influence of each energy term of a hybrid active contour model (ACM). The complete set of the OLMs for a set of training images is defined simultaneously via an optimization scheme. At the same time, the optimal ACM parameters are also calculated. Therefore, heuristic parameter fine-tuning is not required. Training OLMs are subsequently combined, by applying an extended multiatlas concept, to produce the OLMs that are anatomically more suitable to the test image. The proposed algorithm was tested on three different and publicly available data sets. Its accuracy was compared with that of state-of-the-art methods demonstrating the efficacy and robustness of the proposed method. PMID:27170866

  1. High resolution 3D P wave velocity structure beneath Tenerife Island (Canary Islands, Spain) based on tomographic inversion of active-source data

    NASA Astrophysics Data System (ADS)

    GarcíA-Yeguas, Araceli; Koulakov, Ivan; IbáñEz, Jesús M.; Rietbrock, A.

    2012-09-01

    We present a high resolution 3 dimensional (3D) P wave velocity model for Tenerife Island, Canaries, covering the top of Teide volcano (3,718 m a.s.l.) down to around 8 km below sea level (b.s.l). The tomographic inversion is based on a large data set of travel times obtained from a 3D active seismic experiment using offshore shots (air guns) recorded at more than 100 onshore seismic stations. The obtained seismic velocity structure is strongly heterogeneous with significant (up to 40%) lateral variations. The main volcanic structure of the Las Cañadas-Teide-Pico Viejo Complex (CTPVC) is characterized by a high P wave velocity body, similar to many other stratovolcanoes. The presence of different high P wave velocity regions inside the CTPVC may be related to the geological and volcanological evolution of the system. The presence of high P wave velocities at the center of the island is interpreted as evidence for a single central volcanic source for the formation of Tenerife. Furthermore, reduced P wave velocities are found in a small confined region in CTPVC and are more likely related to hydrothermal alteration, as indicated by the existence of fumaroles, than to the presence of a magma chamber beneath the system. In the external regions, surrounding CTPVC a few lower P wave velocity regions can be interpreted as fractured zones, hydrothermal alterations, porous materials and thick volcaniclastic deposits.

  2. Left-ventricle segmentation in real-time 3D echocardiography using a hybrid active shape model and optimal graph search approach

    NASA Astrophysics Data System (ADS)

    Zhang, Honghai; Abiose, Ademola K.; Campbell, Dwayne N.; Sonka, Milan; Martins, James B.; Wahle, Andreas

    2010-03-01

    Quantitative analysis of the left ventricular shape and motion patterns associated with left ventricular mechanical dyssynchrony (LVMD) is essential for diagnosis and treatment planning in congestive heart failure. Real-time 3D echocardiography (RT3DE) used for LVMD analysis is frequently limited by heavy speckle noise or partially incomplete data, thus a segmentation method utilizing learned global shape knowledge is beneficial. In this study, the endocardial surface of the left ventricle (LV) is segmented using a hybrid approach combining active shape model (ASM) with optimal graph search. The latter is used to achieve landmark refinement in the ASM framework. Optimal graph search translates the 3D segmentation into the detection of a minimum-cost closed set in a graph and can produce a globally optimal result. Various information-gradient, intensity distributions, and regional-property terms-are used to define the costs for the graph search. The developed method was tested on 44 RT3DE datasets acquired from 26 LVMD patients. The segmentation accuracy was assessed by surface positioning error and volume overlap measured for the whole LV as well as 16 standard LV regions. The segmentation produced very good results that were not achievable using ASM or graph search alone.

  3. Coastal cliff monitoring and analysis of mass wasting processes with the application of terrestrial laser scanning: A case study of Rügen, Germany

    NASA Astrophysics Data System (ADS)

    Kuhn, Dirk; Prüfer, Steffen

    2014-05-01

    An active landslide, affecting a composite sea cliff section of the island of Rügen, Germany was investigated and monitored using terrestrial laser scanning. Biannual surveys were performed from 2006 to 2011. High resolution scans were used to delineate landslide extensions, monitor the spatial and temporal changes, and evaluate the processes of mass wasting along the cliff section. Between May 2006 and May 2011 the multi-temporal analysis documents a strong landward progression of the landslide that resulted in an increase in the planimetric area by 32%. In the same period, 9365 m3 of soil volume has been removed primarily due to rainfall triggered surface runoff erosion and the resulting slumping. This volume loss correlates with the preferential erosion of weak and unconsolidated sediments building up the major part of the cliff face. This produced a maximum cliff top retreat of 17.32 m with an average retreat rate of 3.46 m yr- 1. The quantification of the mass wasting processes and cliff top retreat demonstrates the substantial effect of local cliff lithology on slope stability, and supports a prognosis of the near future development of the landslide. Furthermore, the absence of local wave attack indicators or human activity highlights the major role of subaerial processes in the erosion of the sea cliff. These results can be transferred to cliff locations in the urbanised cliff top area nearby that currently suffer similar cliff erosion processes and episodic events of large cliff top retreats. The study therefore provides reliable data for the local authorities in charge of hazard assessment and planning and implementation of adaptive countermeasures.

  4. Resveratrol, Acetyl-Resveratrol, and Polydatin Exhibit Antigrowth Activity against 3D Cell Aggregates of the SKOV-3 and OVCAR-8 Ovarian Cancer Cell Lines

    PubMed Central

    Hogg, Simon J.; Chitcholtan, Kenny; Hassan, Wafaa; Sykes, Peter H.; Garrill, Ashley

    2015-01-01

    Resveratrol has aroused significant scientific interest as it has been claimed that it exhibits a spectrum of health benefits. These include effects as an anti-inflammatory and an antitumour compound. The purpose of this study was to investigate and compare any potential antigrowth effects of resveratrol and two of its derivatives, acetyl-resveratrol and polydatin, on 3D cell aggregates of the EGFR/Her-2 positive and negative ovarian cancer cell lines SKOV-3 and OVCAR-8, respectively. Results showed that resveratrol and acetyl-resveratrol reduced cell growth in the SKOV-3 and OVCAR-8 in a dose-dependant manner. The growth reduction was mediated by the induction of apoptosis via the cleavage of poly(ADP-ribose) polymerase (PARP-1). At lower concentrations, 5 and 10 µM, resveratrol, acetyl-resveratrol, and polydatin were less effective than higher concentrations, 50 and 100 µM. In SKOV-3 line, at higher concentrations, resveratrol and polydatin significantly reduced the phosphorylation of Her-2 and EGFR and the expression of Erk. Acetyl-resveratrol, on the other hand, did not change the activation of Her-2 and EGFR. Resveratrol, acetyl-resveratrol, and polydatin suppressed the secretion of VEGF in a dose-dependant fashion. In the OVCAR-8 cell line, resveratrol and acetyl-resveratrol at 5 and 10 µM increased the activation of Erk. Above these concentrations they decreased activation. Polydatin did not produce this effect. This study demonstrates that resveratrol and its derivatives may inhibit growth of 3D cell aggregates of ovarian cancer cell lines via different signalling molecules. Resveratrol and its derivatives, therefore, warrant further in vivo evaluation to assess their potential clinical utility. PMID:26617640

  5. Multiviewer 3D monitor

    NASA Astrophysics Data System (ADS)

    Kostrzewski, Andrew A.; Aye, Tin M.; Kim, Dai Hyun; Esterkin, Vladimir; Savant, Gajendra D.

    1998-09-01

    Physical Optics Corporation has developed an advanced 3-D virtual reality system for use with simulation tools for training technical and military personnel. This system avoids such drawbacks of other virtual reality (VR) systems as eye fatigue, headaches, and alignment for each viewer, all of which are due to the need to wear special VR goggles. The new system is based on direct viewing of an interactive environment. This innovative holographic multiplexed screen technology makes it unnecessary for the viewer to wear special goggles.

  6. 3D Audio System

    NASA Technical Reports Server (NTRS)

    1992-01-01

    Ames Research Center research into virtual reality led to the development of the Convolvotron, a high speed digital audio processing system that delivers three-dimensional sound over headphones. It consists of a two-card set designed for use with a personal computer. The Convolvotron's primary application is presentation of 3D audio signals over headphones. Four independent sound sources are filtered with large time-varying filters that compensate for motion. The perceived location of the sound remains constant. Possible applications are in air traffic control towers or airplane cockpits, hearing and perception research and virtual reality development.

  7. Sulfonamide derivatives containing dihydropyrazole moieties selectively and potently inhibit MMP-2/MMP-9: Design, synthesis, inhibitory activity and 3D-QSAR analysis.

    PubMed

    Yan, Xiao-Qiang; Wang, Zhong-Chang; Li, Zhen; Wang, Peng-Fei; Qiu, Han-Yue; Chen, Long-Wang; Lu, Xiao-Yuan; Lv, Peng-Cheng; Zhu, Hai-Liang

    2015-10-15

    New series of sulfonamide derivatives containing a dihydropyrazole moieties inhibitors of MMP-2/MMP-9 were discovered using structure-based drug design. Synthesis, antitumor activity, structure-activity relationship and optimization of physicochemical properties were described. In vitro the bioassay results revealed that most target compounds showed potent inhibitory activity in the enzymatic and cellular assays. Among the compounds, compound 3i exhibited the most potent inhibitory activity with IC50 values of 0.21 μM inhibiting MMP-2 and 1.87 μM inhibiting MMP-9, comparable to the control positive compound CMT-1 (1.26 μM, 2.52 μM). Docking simulation was performed to position compound 3i into the MMP-2 active site to determine the probable binding pose. Docking simulation was further performed to position compound 3i into the MMP-2 active site to determine the probable binding model the 3D-QSAR models were built for reasonable design of MMP-2/MMP-9 inhibitors at present and in future. PMID:26346367

  8. 3D Surgical Simulation

    PubMed Central

    Cevidanes, Lucia; Tucker, Scott; Styner, Martin; Kim, Hyungmin; Chapuis, Jonas; Reyes, Mauricio; Proffit, William; Turvey, Timothy; Jaskolka, Michael

    2009-01-01

    This paper discusses the development of methods for computer-aided jaw surgery. Computer-aided jaw surgery allows us to incorporate the high level of precision necessary for transferring virtual plans into the operating room. We also present a complete computer-aided surgery (CAS) system developed in close collaboration with surgeons. Surgery planning and simulation include construction of 3D surface models from Cone-beam CT (CBCT), dynamic cephalometry, semi-automatic mirroring, interactive cutting of bone and bony segment repositioning. A virtual setup can be used to manufacture positioning splints for intra-operative guidance. The system provides further intra-operative assistance with the help of a computer display showing jaw positions and 3D positioning guides updated in real-time during the surgical procedure. The CAS system aids in dealing with complex cases with benefits for the patient, with surgical practice, and for orthodontic finishing. Advanced software tools for diagnosis and treatment planning allow preparation of detailed operative plans, osteotomy repositioning, bone reconstructions, surgical resident training and assessing the difficulties of the surgical procedures prior to the surgery. CAS has the potential to make the elaboration of the surgical plan a more flexible process, increase the level of detail and accuracy of the plan, yield higher operative precision and control, and enhance documentation of cases. Supported by NIDCR DE017727, and DE018962 PMID:20816308

  9. SU-E-J-141: Activity-Equivalent Path Length Approach for the 3D PET-Based Dose Reconstruction in Proton Therapy

    SciTech Connect

    Attili, A; Vignati, A; Giordanengo, S; Kraan, A; Dalmasso, F; Battistoni, G

    2015-06-15

    Purpose: Ion beam therapy is sensitive to uncertainties from treatment planning and dose delivery. PET imaging of induced positron emitter distributions is a practical approach for in vivo, in situ verification of ion beam treatments. Treatment verification is usually done by comparing measured activity distributions with reference distributions, evaluated in nominal conditions. Although such comparisons give valuable information on treatment quality, a proper clinical evaluation of the treatment ultimately relies on the knowledge of the actual delivered dose. Analytical deconvolution methods relating activity and dose have been studied in this context, but were not clinically applied. In this work we present a feasibility study of an alternative approach for dose reconstruction from activity data, which is based on relating variations in accumulated activity to tissue density variations. Methods: First, reference distributions of dose and activity were calculated from the treatment plan and CT data. Then, the actual measured activity data were cumulatively matched with the reference activity distributions to obtain a set of activity-equivalent path lengths (AEPLs) along the rays of the pencil beams. Finally, these AEPLs were used to deform the original dose distribution, yielding the actual delivered dose. The method was tested by simulating a proton therapy treatment plan delivering 2 Gy on a homogeneous water phantom (the reference), which was compared with the same plan delivered on a phantom containing inhomogeneities. Activity and dose distributions were were calculated by means of the FLUKA Monte Carlo toolkit. Results: The main features of the observed dose distribution in the inhomogeneous situation were reproduced using the AEPL approach. Variations in particle range were reproduced and the positions, where these deviations originated, were properly identified. Conclusions: For a simple inhomogeneous phantom the 3D dose reconstruction from PET-activity

  10. Evaluation of the ECAT EXACT HR{sup +} 3D PET scanner in {sup 15}O-water brain activation studies

    SciTech Connect

    Moreno-Cantu, J.J.; Thompson, C.J.; Zatorre, R.J.

    1996-12-31

    We evaluated the performance of the ECAT EXACT HR{sup +} 3D whole body PET scanner when employed to measure brain function using {sup 15}O-water-bolus activation protocols in single data acquisition sessions. Using vibrotactile and auditory stimuli as independent activation tasks, we studied the scanner`s performance under different imaging conditions in four healthy volunteers. Cerebral blood flow images were acquired from each volunteer using {sup 15}O-water-bolus injections of activity varying from 5 to 20mCi. Performance characteristics. The scanner`s dead time grew linearly with injected dose from 10% to 25%. Random events varied from 30% to 50% of the detected events. Scattered events were efficiently corrected at all doses. Noise-effective-count curves plateau at about 15mCi. One-session 12-injection bolus PET activation protocol. Using an acquisition protocol that accounts for the scanner`s performance and the practical aspects of imaging volunteers and patients in one session, we assessed the correlation between the statistical significance of activation foci and the dose per injection used The one-session protocol employs 12 bolus injections per subject. We present evidence suggesting that 15-20mCi is the optimal dose per injection to be used routinely in one-time scanning sessions.

  11. Docking and 3D-QSAR (quantitative structure activity relationship) studies of flavones, the potent inhibitors of p-glycoprotein targeting the nucleotide binding domain.

    PubMed

    Kothandan, Gugan; Gadhe, Changdev G; Madhavan, Thirumurthy; Choi, Cheol Hee; Cho, Seung Joo

    2011-09-01

    In order to explore the interactions between flavones and P-gp, in silico methodologies such as docking and 3D-QSAR were performed. CoMFA and CoMSIA analyses were done using ligand based and receptor guided alignment schemes. Validation statistics include leave-one-out cross-validated R(2) (q(2)), internal prediction parameter by progressive scrambling (Q(*2)), external prediction with test set. They show that models derived from this study are quite robust. Ligand based CoMFA (q(2) = 0.747, Q(*2) = 0.639, r(pred)(2)=0.802) and CoMSIA model (q(2) = 0.810, Q(*2) = 0.676, r(pred)(2)=0.785) were developed using atom by atom matching. Receptor guided CoMFA (q(2) = 0.712, Q(*2) = 0.497, r(pred)(2) = 0.841) and for CoMSIA (q(2) = 0.805, Q(*2) = 0.589, r(pred)(2) = 0.937) models were developed by docking of highly active flavone into the proposed NBD (nucleotide binding domain) of P-gp. The 3D-QSAR models generated here predicted that hydrophobic and steric parameters are important for activity toward P-gp. Our studies indicate the important amino acid in NBD crucial for binding in accordance with the previous results. This site forms a hydrophobic site. Since flavonoids have potential without toxicity, we propose to inspect this hydrophobic site including Asn1043 and Asp1049 should be considered for future inhibitor design. PMID:21723648

  12. Flora of the Orange Cliffs of Utah

    SciTech Connect

    Shultz, L.M.; Neely, E.E.; Tuhy, J.S.

    1987-04-30

    The Orange Cliffs area, an area rich in oil sands deposits and defined here as part of the Colorado Plateau floristic province, harbors approximately 209 species in 123 genera and 49 families. Because of the potential of exploitation of the oil sands deposits in the area, a species checklist was made and a discussion of physical and floristic aspects of the region is given here. The flora is compared statistically to the San Rafael Swell flora, which is also a subset of the Colorado Plateau. They define six vegetation types and three edaphic communities; these are described and mapped. Of eleven endemic plant species in the Orange Cliffs, three are local and rare. Sites for Astragalus nidularius, A. moencoppensis, and Xylorhiza glabriuscula var. linearifolia are discussed and mapped. 24 references, 3 figures, 3 tables.

  13. Inshore analyse of the morphostructural evolution of the coastal cliffs of Bessin, Basse-Normandie, France

    NASA Astrophysics Data System (ADS)

    Vioget, Alizée; Costa, Stéphane; Derron, Marc-Henri; Jaboyedoff, Michel; Maquaire, Olivier; Michoud, Clément

    2014-05-01

    'retreat evolves in successive leaps and bounds. Thus, results won't be significant if there aren't long period observations. Therefore, the documents used consist of orthophotos and oblique aerial pictures that cover a period from 1947 to 2009. This step shows higher retreat velocity in marls (0.15-0.2 m/yr) substratum than in limestones (0.05-0.1 m/yr). Finally, we also focused on a complex landslide that happened in May 2013 near Cape Manvieux. We aim to reconstruct the kinematic and to determine the mass volume that has moved. For that purpose, a terrestrial LiDAR (Optech Ilris-3D ER) acquisition of the instability was performed in July 2013. The 3D point cloud allows a quite precise estimation of involved volume. Moreover, LiDAR data and field observations let us think that the current state of the instability was created by multiple events and is a complex mix of creeping marls and toppling of limestone destabilised by a back subvertical discontinuity which is parallel to the coastline. In conclusion, the studies on morphostructural evolution of the cliffs with geomorphological field studies and LiDAR acquisition lead to a better comprehension of coastline retreat in Normandy.

  14. 3D polarimetric purity

    NASA Astrophysics Data System (ADS)

    Gil, José J.; San José, Ignacio

    2010-11-01

    From our previous definition of the indices of polarimetric purity for 3D light beams [J.J. Gil, J.M. Correas, P.A. Melero and C. Ferreira, Monogr. Semin. Mat. G. de Galdeano 31, 161 (2004)], an analysis of their geometric and physical interpretation is presented. It is found that, in agreement with previous results, the first parameter is a measure of the degree of polarization, whereas the second parameter (called the degree of directionality) is a measure of the mean angular aperture of the direction of propagation of the corresponding light beam. This pair of invariant, non-dimensional, indices of polarimetric purity contains complete information about the polarimetric purity of a light beam. The overall degree of polarimetric purity is obtained as a weighted quadratic average of the degree of polarization and the degree of directionality.

  15. 3D field harmonics

    SciTech Connect

    Caspi, S.; Helm, M.; Laslett, L.J.

    1991-03-30

    We have developed an harmonic representation for the three dimensional field components within the windings of accelerator magnets. The form by which the field is presented is suitable for interfacing with other codes that make use of the 3D field components (particle tracking and stability). The field components can be calculated with high precision and reduced cup time at any location (r,{theta},z) inside the magnet bore. The same conductor geometry which is used to simulate line currents is also used in CAD with modifications more readily available. It is our hope that the format used here for magnetic fields can be used not only as a means of delivering fields but also as a way by which beam dynamics can suggest correction to the conductor geometry. 5 refs., 70 figs.

  16. 'Bonneville' in 3-D!

    NASA Technical Reports Server (NTRS)

    2004-01-01

    The Mars Exploration Rover Spirit took this 3-D navigation camera mosaic of the crater called 'Bonneville' after driving approximately 13 meters (42.7 feet) to get a better vantage point. Spirit's current position is close enough to the edge to see the interior of the crater, but high enough and far enough back to get a view of all of the walls. Because scientists and rover controllers are so pleased with this location, they will stay here for at least two more martian days, or sols, to take high resolution panoramic camera images of 'Bonneville' in its entirety. Just above the far crater rim, on the left side, is the rover's heatshield, which is visible as a tiny reflective speck.

  17. Biocompatible 3D Matrix with Antimicrobial Properties.

    PubMed

    Ion, Alberto; Andronescu, Ecaterina; Rădulescu, Dragoș; Rădulescu, Marius; Iordache, Florin; Vasile, Bogdan Ștefan; Surdu, Adrian Vasile; Albu, Madalina Georgiana; Maniu, Horia; Chifiriuc, Mariana Carmen; Grumezescu, Alexandru Mihai; Holban, Alina Maria

    2016-01-01

    The aim of this study was to develop, characterize and assess the biological activity of a new regenerative 3D matrix with antimicrobial properties, based on collagen (COLL), hydroxyapatite (HAp), β-cyclodextrin (β-CD) and usnic acid (UA). The prepared 3D matrix was characterized by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Microscopy (FT-IRM), Transmission Electron Microscopy (TEM), and X-ray Diffraction (XRD). In vitro qualitative and quantitative analyses performed on cultured diploid cells demonstrated that the 3D matrix is biocompatible, allowing the normal development and growth of MG-63 osteoblast-like cells and exhibited an antimicrobial effect, especially on the Staphylococcus aureus strain, explained by the particular higher inhibitory activity of usnic acid (UA) against Gram positive bacterial strains. Our data strongly recommend the obtained 3D matrix to be used as a successful alternative for the fabrication of three dimensional (3D) anti-infective regeneration matrix for bone tissue engineering. PMID:26805790

  18. Fabrication of 3D Silicon Sensors

    SciTech Connect

    Kok, A.; Hansen, T.E.; Hansen, T.A.; Lietaer, N.; Summanwar, A.; Kenney, C.; Hasi, J.; Da Via, C.; Parker, S.I.; /Hawaii U.

    2012-06-06

    Silicon sensors with a three-dimensional (3-D) architecture, in which the n and p electrodes penetrate through the entire substrate, have many advantages over planar silicon sensors including radiation hardness, fast time response, active edge and dual readout capabilities. The fabrication of 3D sensors is however rather complex. In recent years, there have been worldwide activities on 3D fabrication. SINTEF in collaboration with Stanford Nanofabrication Facility have successfully fabricated the original (single sided double column type) 3D detectors in two prototype runs and the third run is now on-going. This paper reports the status of this fabrication work and the resulted yield. The work of other groups such as the development of double sided 3D detectors is also briefly reported.

  19. 3D modelling of the active normal fault network in the Apulian Ridge (Eastern Mediterranean Sea): Integration of seismic and bathymetric data with implicit surface methods

    NASA Astrophysics Data System (ADS)

    Bistacchi, Andrea; Pellegrini, Caludio; Savini, Alessandra; Marchese, Fabio

    2016-04-01

    The Apulian ridge (North-eastern Ionian Sea, Mediterranean), interposed between the facing Apennines and Hellenides subduction zones (to the west and east respectively), is characterized by thick cretaceous carbonatic sequences and discontinuous tertiary deposits crosscut by a penetrative network of NNW-SSE normal faults. These are exposed onshore in Puglia, and are well represented offshore in a dataset composed of 2D seismics and wells collected by oil companies from the '60s to the '80s, more recent seismics collected during research projects in the '90s, recent very high resolution seismics (VHRS - Sparker and Chirp-sonar data), multibeam echosounder bathymetry, and sedimentological and geo-chronological analyses of sediment samples collected on the seabed. Faults are evident in 2D seismics at all scales, and their along-strike geometry and continuity can be characterized with multibeam bathymetric data, which show continuous fault scarps on the seabed (only partly reworked by currents and covered by landslides). Fault scarps also reveal the finite displacement accumulated in the Holocene-Pleistocene. We reconstructed a 3D model of the fault network and suitable geological boundaries (mainly unconformities due to the discontinuous distribution of quaternary and tertiary sediments) with implicit surface methods implemented in SKUA/GOCAD. This approach can be considered very effective and allowed reconstructing in details complex structures, like the frequent relay zones that are particularly well imaged by seafloor geomorphology. Mutual cross-cutting relationships have been recognized between fault scarps and submarine mass-wasting deposits (Holocene-Pleistocene), indicating that, at least in places, these features are coeval, hence the fault network should be considered active. At the regional scale, the 3D model allowed measuring the horizontal WSW-ENE stretching, which can be associated to the bending moment applied to the Apulian Plate by the combined effect

  20. Study of the 3D Coronal Magnetic Field of Active Region 11117 Around the Time of a Confined Flare Using a Data-Driven CESE-MHD Model

    NASA Astrophysics Data System (ADS)

    Jiang, C.; Feng, X.; Wu, S.; Hu, Q.

    2012-12-01

    Non-potentiality of the solar coronal magnetic field accounts for the solar explosion like flares and CMEs. We apply a data-driven CESE-MHD model to investigate the three-dimensional (3D) coronal magnetic field of NOAA active region (AR) 11117 around the time of a C-class confined flare occurred on 2010 October 25. The CESE-MHD model, based on the spacetime conservation-element and solution-element scheme, is designed to focus on the magnetic-field evolution and to consider a simplified solar atomsphere with finite plasma β. Magnetic vector-field data derived from the observations at the photoshpere is inputted directly to constrain the model. Assuming that the dynamic evolution of the coronal magnetic field can be approximated by successive equilibria, we solve a time sequence of MHD equilibria basing on a set of vector magnetograms for AR 11117 taken by the Helioseismic and Magnetic Imager (HMI) on board the Solar Dynamic Observatory (SDO) around the time of flare. The model qualitatively reproduces the basic structures of the 3D magnetic field, as supported by the visual similarity between the field lines and the coronal loops observed by the Atmospheric Imaging Assembly (AIA), which shows that the coronal field can indeed be well characterized by the MHD equilibrium in most time. The magnetic configuration changes very limited during the studied time interval of two hours. A topological analysis reveals that the small flare is correlated with a bald patch (BP, where the magnetic field is tangent to the photoshpere), suggesting that the energy release of the flare can be understood by magnetic reconnection associated with the BP separatrices. The total magnetic flux and energy keep increasing slightly in spite of the flare, while the magnetic free energy drops during the flare with an amount of 1.7 × 1030 erg, which can be interpreted as the energy budget released by the minor C-class flare.

  1. Kinematic Analysis of Healthy Hips during Weight-Bearing Activities by 3D-to-2D Model-to-Image Registration Technique

    PubMed Central

    Hara, Daisuke; Nakashima, Yasuharu; Hamai, Satoshi; Higaki, Hidehiko; Ikebe, Satoru; Shimoto, Takeshi; Hirata, Masanobu; Kanazawa, Masayuki; Kohno, Yusuke; Iwamoto, Yukihide

    2014-01-01

    Dynamic hip kinematics during weight-bearing activities were analyzed for six healthy subjects. Continuous X-ray images of gait, chair-rising, squatting, and twisting were taken using a flat panel X-ray detector. Digitally reconstructed radiographic images were used for 3D-to-2D model-to-image registration technique. The root-mean-square errors associated with tracking the pelvis and femur were less than 0.3 mm and 0.3° for translations and rotations. For gait, chair-rising, and squatting, the maximum hip flexion angles averaged 29.6°, 81.3°, and 102.4°, respectively. The pelvis was tilted anteriorly around 4.4° on average during full gait cycle. For chair-rising and squatting, the maximum absolute value of anterior/posterior pelvic tilt averaged 12.4°/11.7° and 10.7°/10.8°, respectively. Hip flexion peaked on the way of movement due to further anterior pelvic tilt during both chair-rising and squatting. For twisting, the maximum absolute value of hip internal/external rotation averaged 29.2°/30.7°. This study revealed activity dependent kinematics of healthy hip joints with coordinated pelvic and femoral dynamic movements. Kinematics' data during activities of daily living may provide important insight as to the evaluating kinematics of pathological and reconstructed hips. PMID:25506056

  2. Design, synthesis, anti-tumor activity, and molecular modeling of quinazoline and pyrido[2,3-d]pyrimidine derivatives targeting epidermal growth factor receptor.

    PubMed

    Hou, Ju; Wan, Shanhe; Wang, Guangfa; Zhang, Tingting; Li, Zhonghuang; Tian, Yuanxin; Yu, Yonghuan; Wu, Xiaoyun; Zhang, Jiajie

    2016-08-01

    Three series of novel quinazoline and pyrido[2,3-d]pyrimidine derivatives were designed, synthesized and evaluated for their ability to inhibit EGFR tyrosine kinase and a panel of five human cancer cell lines (MCF-7, A549, BT-474, SK-BR-3, and MDA-MB-231). Bioassay results indicated that five of these prepared compounds (12c-12e and 13c-13d) exhibited remarkably higher inhibitory activities against EGFR and SK-BR-3 cell line. Compounds 12c and 12e displayed the most potent EGFR inhibitory activity (IC50 = 2.97 nM and 3.58 nM, respectively) and good anti-proliferative effect against SK-BR-3 cell with the IC50 values of 3.10 μM and 5.87 μM, respectively. Furthermore, molecular docking and molecular dynamics simulation studies verified that compound 12c and 12e shared similar binding pattern with gefitinib in the binding pocket of EGFR. MM-GBSA binding free energy revealed that the compound 12c and 12e have almost the same inhibitory activity against EGFR as gefitinib, and that the dominating effect of van der Waals interactions drives the binding process. PMID:27132165

  3. Ground Penetrating Radar (GPR) Imaging to Distinguish Active from Inactive Sinkholes in Covered Karst Terrain: Results from Field Data and 3D FDTD Modeling

    NASA Astrophysics Data System (ADS)

    Gooch, B. T.; Kruse, S. E.

    2009-12-01

    Ground penetrating radar (GPR) is widely used to identify locations of sinkholes in covered karst terrain in Florida. Some sinkholes serve as hydraulic conduits between the surficial and underlying aquifers. Their role is critical in determining the surficial aquifer response to pumping in deeper aquifers. Improved methods for discriminating between hydraulically active sinkholes and plugged sinkholes could help regional water management. In the covered karst of west-central Florida a clay-rich weathering horizon forms over the limestone. The clay-rich layer is in turn overlain by surficial sands. Ground penetrating radar profiles typically show a strong reflector from the top of clay-rich horizon as well as internal layering within sands. Active sinkholes are expected to have sandy conduits that broach the clay layer, and perhaps layering in the overlying sand indicative of ongoing subsidence. Three dimensional simulations of GPR profiles over sinkhole with and without conduits were run with the finite-difference time-domain (FDTD) program GprMax. Results from the synthetic surveys were then processed with standard techniques, including migration. The modeling confirms that conduits appear in GPR records primarily as gaps in the return from the clay layer. The modeling also shows that non-traditional survey geometries (varying antenna spacing and orientation) are unlikely to recover more information than traditional proximal transmitter-receiver separation. We also examine GPR profiles and 3D grids over a set of active and inactive sinkholes in Tampa, Florida. Preliminary analysis suggests that active sinks may present more identifiable gaps in the overlying clay layer, but consistent differences in structure of active and inactive sinkholes are not easily discerned. Other geophysical methods may prove to be more helpful in discerning the presence or absence of active conduits in these situations.

  4. Coastal cliff recession: the use of probabilistic prediction methods

    NASA Astrophysics Data System (ADS)

    Lee, E. M.; Hall, J. W.; Meadowcroft, I. C.

    2001-10-01

    A range of probabilistic methods is introduced for predicting coastal cliff recession, which provide a means of demonstrating the potential variability in such predictions. They form the basis for risk-based land-use planning, cliff management and engineering decision-making. Examples of probabilistic models are presented for a number of different cliff settings: the simulation of recession on eroding cliffs; the use of historical records and statistical experiments to model the behaviour of cliffs affected by rare, episodic landslide events; the adaptation of an event tree approach to assess the probability of failure of protected cliffs, taking into account the residual life of the existing defences; and the evaluation of the probability of landslide reactivation in areas of pre-existing landslide systems. These methods are based on a geomorphological assessment of the episodic nature of the recession process, together with historical records.

  5. Deriving mechanisms and thresholds for cliff retreat in soft-rock cliffs under changing climates: Rapidly retreating cliffs of the Suffolk coast, UK

    NASA Astrophysics Data System (ADS)

    Brooks, S. M.; Spencer, T.; Boreham, S.

    2012-06-01

    Understanding changing thresholds and mechanisms for retreat in soft rock cliffs is important under changing climates. This can be achieved through combining detailed field observation, long-term process and morphological monitoring and numerical modelling. The cliffs of the Suffolk coast, southern North Sea have exhibited long-term (1883-2010) recession rates of 3.5 m a- 1, rising to 4.7 m a- 1 in the period 1993-2010. Annual to biannual ground survey data, and the application of GIS techniques to digitised records of changing shoreline position from historic maps and aerial photography, reveal considerable decadal-scale variations in cliff recession, within which are nested inter-annual fluctuations in rates of retreat. Archival datasets on significant periods of onshore winds and their interaction with high water levels (including the incidence of storm surges) and rainstorm events are used to determine thresholds for cliff base erosion and its propagation upwards through the cliff profile. In addition, the ‘GEO-Slope' dynamic coupled hydrology-stability model is used to establish thresholds for cliff face failures driven by variations in rainfall inputs. Retreat mechanisms are complex, governed by cliff geology, both as a primary control on suction loss and through its interaction with basal marine conditions. The study allows a general model of cliff retreat for soft rock cliffs to be put forward, whereby a resistant basal platform is overlain by more erodible, weakly and moderately cemented sands and gravels. In this model, the varying balance between marine and terrestrial forcing factors are reflected in low (< 4 m a- 1), intermediate (4-7 m a- 1) and high (> 7 m a- 1) modes of cliff retreat.

  6. Interactive 3D Mars Visualization

    NASA Technical Reports Server (NTRS)

    Powell, Mark W.

    2012-01-01

    The Interactive 3D Mars Visualization system provides high-performance, immersive visualization of satellite and surface vehicle imagery of Mars. The software can be used in mission operations to provide the most accurate position information for the Mars rovers to date. When integrated into the mission data pipeline, this system allows mission planners to view the location of the rover on Mars to 0.01-meter accuracy with respect to satellite imagery, with dynamic updates to incorporate the latest position information. Given this information so early in the planning process, rover drivers are able to plan more accurate drive activities for the rover than ever before, increasing the execution of science activities significantly. Scientifically, this 3D mapping information puts all of the science analyses to date into geologic context on a daily basis instead of weeks or months, as was the norm prior to this contribution. This allows the science planners to judge the efficacy of their previously executed science observations much more efficiently, and achieve greater science return as a result. The Interactive 3D Mars surface view is a Mars terrain browsing software interface that encompasses the entire region of exploration for a Mars surface exploration mission. The view is interactive, allowing the user to pan in any direction by clicking and dragging, or to zoom in or out by scrolling the mouse or touchpad. This set currently includes tools for selecting a point of interest, and a ruler tool for displaying the distance between and positions of two points of interest. The mapping information can be harvested and shared through ubiquitous online mapping tools like Google Mars, NASA WorldWind, and Worldwide Telescope.

  7. Prominent rocks - 3D

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Many prominent rocks near the Sagan Memorial Station are featured in this image, taken in stereo by the Imager for Mars Pathfinder (IMP) on Sol 3. 3D glasses are necessary to identify surface detail. Wedge is at lower left; Shark, Half-Dome, and Pumpkin are at center. Flat Top, about four inches high, is at lower right. The horizon in the distance is one to two kilometers away.

    Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

    Click below to see the left and right views individually. [figure removed for brevity, see original site] Left [figure removed for brevity, see original site] Right

  8. 'Diamond' in 3-D

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This 3-D, microscopic imager mosaic of a target area on a rock called 'Diamond Jenness' was taken after NASA's Mars Exploration Rover Opportunity ground into the surface with its rock abrasion tool for a second time.

    Opportunity has bored nearly a dozen holes into the inner walls of 'Endurance Crater.' On sols 177 and 178 (July 23 and July 24, 2004), the rover worked double-duty on Diamond Jenness. Surface debris and the bumpy shape of the rock resulted in a shallow and irregular hole, only about 2 millimeters (0.08 inch) deep. The final depth was not enough to remove all the bumps and leave a neat hole with a smooth floor. This extremely shallow depression was then examined by the rover's alpha particle X-ray spectrometer.

    On Sol 178, Opportunity's 'robotic rodent' dined on Diamond Jenness once again, grinding almost an additional 5 millimeters (about 0.2 inch). The rover then applied its Moessbauer spectrometer to the deepened hole. This double dose of Diamond Jenness enabled the science team to examine the rock at varying layers. Results from those grindings are currently being analyzed.

    The image mosaic is about 6 centimeters (2.4 inches) across.

  9. Trends in activity for the water electrolyser reactions on 3d M(Ni,Co,Fe,Mn) hydr(oxy)oxide catalysts

    NASA Astrophysics Data System (ADS)

    Subbaraman, Ram; Tripkovic, Dusan; Chang, Kee-Chul; Strmcnik, Dusan; Paulikas, Arvydas P.; Hirunsit, Pussana; Chan, Maria; Greeley, Jeff; Stamenkovic, Vojislav; Markovic, Nenad M.

    2012-06-01

    Design and synthesis of materials for efficient electrochemical transformation of water to molecular hydrogen and of hydroxyl ions to oxygen in alkaline environments is of paramount importance in reducing energy losses in water-alkali electrolysers. Here, using 3d-M hydr(oxy)oxides, with distinct stoichiometries and morphologies in the hydrogen evolution reaction (HER) and the oxygen evolution reaction (OER) regions, we establish the overall catalytic activities for these reaction as a function of a more fundamental property, a descriptor, OH-M2+δ bond strength (0 ≤ δ ≤ 1.5). This relationship exhibits trends in reactivity (Mn < Fe < Co < Ni), which is governed by the strength of the OH-M2+δ energetic (Ni < Co < Fe < Mn). These trends are found to be independent of the source of the OH, either the supporting electrolyte (for the OER) or the water dissociation product (for the HER). The successful identification of these electrocatalytic trends provides the foundation for rational design of ‘active sites’ for practical alkaline HER and OER electrocatalysts.

  10. Pleiotropic Anti-Angiogenic and Anti-Oncogenic Activities of the Novel Mithralog Demycarosyl-3D-ß-D-Digitoxosyl-Mithramycin SK (EC-8042).

    PubMed

    Fernández-Guizán, Azahara; López-Soto, Alejandro; Acebes-Huerta, Andrea; Huergo-Zapico, Leticia; Villa-Álvarez, Mónica; Núñez, Luz-Elena; Morís, Francisco; Gonzalez, Segundo

    2015-01-01

    Demycarosyl-3D-ß-D-digitoxosyl-mithramycin SK (DIG-MSK) is a recently isolated analogue of mithramycin A (MTA) that showed differences with MTA in the DNA binding strength and selectivity. These differences correlated with a better therapeutic index and less toxicity in animal studies. Herein, we show that DIG-MSK displays a potent anti-tumor activity against different types of cancer cell lines, ovarian tumor cells being particularly sensitive to this drug. Of relevance, DIG-MSK exerts low toxicity on fibroblasts and peripheral blood mononuclear cells, this toxicity being significantly lower than that of MTA. In correlation with its antitumor activity, DIG-MSK strongly inhibited Sp1-mediated transcription and endogenous Sp1 mRNA expression, which correlated with the inhibition of the expression of key Sp1-regulated genes involved in tumorigenesis, including VEGFA, BCL2L1 (Bcl-XL), hTERT, BRCA2, MYC and SRC in several ovarian cells. Significantly, DIG-MSK was a stronger inhibitor of VEGFA expression than MTA. Accordingly, DIG-MSK also exhibited potent anti-angiogenic activity on microvascular endothelial cells. Likewise, it significantly inhibited the gene expression of VEGFR1, VEGFR2, FGFR, PDGFB and PDGFRA and, additionally, it induced the expression of the anti-angiogenic factors angiostatin and tunstatin. These effects correlated with a pro-apoptotic effect on proliferating microvascular endothelial cells and the inhibition of the formation of endothelial capillary structures. Overall, the pleiotropic activity of DIG-MSK in inhibiting key oncogenic and angiogenic pathways, together with its low toxicity profile, highlight the therapeutic potential of this new drug. PMID:26536461

  11. Pleiotropic Anti-Angiogenic and Anti-Oncogenic Activities of the Novel Mithralog Demycarosyl-3D-ß-D-Digitoxosyl-Mithramycin SK (EC-8042)

    PubMed Central

    Fernández-Guizán, Azahara; López-Soto, Alejandro; Acebes-Huerta, Andrea; Huergo-Zapico, Leticia; Villa-Álvarez, Mónica; Núñez, Luz-Elena; Morís, Francisco; Gonzalez, Segundo

    2015-01-01

    Demycarosyl-3D-ß-D-digitoxosyl-mithramycin SK (DIG-MSK) is a recently isolated analogue of mithramycin A (MTA) that showed differences with MTA in the DNA binding strength and selectivity. These differences correlated with a better therapeutic index and less toxicity in animal studies. Herein, we show that DIG-MSK displays a potent anti-tumor activity against different types of cancer cell lines, ovarian tumor cells being particularly sensitive to this drug. Of relevance, DIG-MSK exerts low toxicity on fibroblasts and peripheral blood mononuclear cells, this toxicity being significantly lower than that of MTA. In correlation with its antitumor activity, DIG-MSK strongly inhibited Sp1-mediated transcription and endogenous Sp1 mRNA expression, which correlated with the inhibition of the expression of key Sp1-regulated genes involved in tumorigenesis, including VEGFA, BCL2L1 (Bcl-XL), hTERT, BRCA2, MYC and SRC in several ovarian cells. Significantly, DIG-MSK was a stronger inhibitor of VEGFA expression than MTA. Accordingly, DIG-MSK also exhibited potent anti-angiogenic activity on microvascular endothelial cells. Likewise, it significantly inhibited the gene expression of VEGFR1, VEGFR2, FGFR, PDGFB and PDGFRA and, additionally, it induced the expression of the anti-angiogenic factors angiostatin and tunstatin. These effects correlated with a pro-apoptotic effect on proliferating microvascular endothelial cells and the inhibition of the formation of endothelial capillary structures. Overall, the pleiotropic activity of DIG-MSK in inhibiting key oncogenic and angiogenic pathways, together with its low toxicity profile, highlight the therapeutic potential of this new drug. PMID:26536461

  12. 3D image of Brittle/Ductile transition in active volcanic area and its implication on seismicity: The Campi Flegrei caldera case study

    NASA Astrophysics Data System (ADS)

    Castaldo, Raffaele; Luca, D'auria; Susi, Pepe; Giuseppe, Solaro; Pietro, Tizzani

    2015-04-01

    The thermo-rheology of the rocks is a crucial aspect to understand the mechanical behavior of the crust in young and tectonically active area. As a consequence, several studies have been performed since last decades in order to understand the role of thermic state in the evolution of volcanic environments. In this context, we analyze the upper crust rheology of the Campi Flegrei active caldera (Southern Italy). Our target is the evaluation of the 3D geometry of the Brittle-Ductile transition beneath the resurgent caldera, by integrating the available geological, geochemical, and geophysical data. We first performed a numerical thermal model by using the a priori geological and geophysical information; than we employ the retrieved isothermal distribution to image the rheological stratification of the shallow crust beneath caldera. In particular, considering both the thermal proprieties and the mechanical heterogeneities of the upper crust, we performed, in a Finite Element environment, a 3D conductive time dependent thermal model through an numerical of solution of the Fourier equation. The dataset consist in temperature measurements recorded in several deep wells. More specifically, the geothermal gradients were measured in seven deep geothermal boreholes, located in three main distinct areas: Mofete, Licola, and San Vito. In addition, we take into account also the heat flow density map at the caldera surface calculated by considering the thermal measurements carried out in 30 shallow water wells. We estimate the isothermal distribution of the crust calibrating two model parameters: the heat production [W], associated to the magma injection episodes in the last 60 kyears within the magma chamber and the heat flow coefficient [W/m2*K] at the external surface. In particular, the optimization procedure has been performed using an exhaustive grid search, to minimize the differences between model and experimental measurements. The achieved results allowed us to

  13. The effect of activity outside the field of view on image quality for a 3D LSO-based whole body PET/CT scanner.

    PubMed

    Matheoud, R; Secco, C; Della Monica, P; Leva, L; Sacchetti, G; Inglese, E; Brambilla, M

    2009-10-01

    The purpose of this study was to quantify the influence of outside field of view (FOV) activity concentration (A(c)(,out)) on the noise equivalent count rate (NECR), scatter fraction (SF) and image quality of a 3D LSO whole-body PET/CT scanner. The contrast-to-noise ratio (CNR) was the figure of merit used to characterize the image quality of PET scans. A modified International Electrotechnical Commission (IEC) phantom was used to obtain SF and counting rates similar to those found in average patients. A scatter phantom was positioned at the end of the modified IEC phantom to simulate an activity that extends beyond the scanner. The modified IEC phantom was filled with (18)F (11 kBq mL(-1)) and the spherical targets, with internal diameter (ID) ranging from 10 to 37 mm, had a target-to-background ratio of 10. PET images were acquired with background activity concentrations into the FOV (A(c)(,bkg)) about 11, 9.2, 6.6, 5.2 and 3.5 kBq mL(-1). The emission scan duration (ESD) was set to 1, 2, 3 and 4 min. The tube inside the scatter phantom was filled with activities to provide A(c)(,out) in the whole scatter phantom of zero, half, unity, twofold and fourfold the one of the modified IEC phantom. Plots of CNR versus the various parameters are provided. Multiple linear regression was employed to study the effects of A(c)(,out) on CNR, adjusted for the presence of variables (sphere ID, A(c)(,bkg) and ESD) related to CNR. The presence of outside FOV activity at the same concentration as the one inside the FOV reduces peak NECR of 30%. The increase in SF is marginal (1.2%). CNR diminishes significantly with increasing outside FOV activity, in the range explored. ESD and A(c)(,out) have a similar weight in accounting for CNR variance. Thus, an experimental law that adjusts the scan duration to the outside FOV activity can be devised. Recovery of CNR loss due to an elevated A(c)(,out) activity seems feasible by modulating the ESD in individual bed positions according to A

  14. Decoding 3D reach and grasp from hybrid signals in motor and premotor cortices: spikes, multiunit activity, and local field potentials

    PubMed Central

    Truccolo, Wilson; Vargas-Irwin, Carlos E.; Donoghue, John P.

    2012-01-01

    Neural activity in motor cortex during reach and grasp movements shows modulations in a broad range of signals from single-neuron spiking activity (SA) to various frequency bands in broadband local field potentials (LFPs). In particular, spatiotemporal patterns in multiband LFPs are thought to reflect dendritic integration of local and interareal synaptic inputs, attentional and preparatory processes, and multiunit activity (MUA) related to movement representation in the local motor area. Nevertheless, the relationship between multiband LFPs and SA, and their relationship to movement parameters and their relative value as brain-computer interface (BCI) control signals, remain poorly understood. Also, although this broad range of signals may provide complementary information channels in primary (MI) and ventral premotor (PMv) areas, areal differences in information have not been systematically examined. Here, for the first time, the amount of information in SA and multiband LFPs was compared for MI and PMv by recording from dual 96-multielectrode arrays while monkeys made naturalistic reach and grasp actions. Information was assessed as decoding accuracy for 3D arm end point and grip aperture kinematics based on SA or LFPs in MI and PMv, or combinations of signal types across areas. In contrast with previous studies with ≤16 simultaneous electrodes, here ensembles of >16 units (on average) carried more information than multiband, multichannel LFPs. Furthermore, reach and grasp information added by various LFP frequency bands was not independent from that in SA ensembles but rather typically less than and primarily contained within the latter. Notably, MI and PMv did not show a particular bias toward reach or grasp for this task or for a broad range of signal types. For BCIs, our results indicate that neuronal ensemble spiking is the preferred signal for decoding, while LFPs and combined signals from PMv and MI can add robustness to BCI control. PMID:22157115

  15. Study of differences in the VEGFR2 inhibitory activities between semaxanib and SU5205 using 3D-QSAR, docking, and molecular dynamics simulations.

    PubMed

    Muñoz, Camila; Adasme, Francisco; Alzate-Morales, Jans H; Vergara-Jaque, Ariela; Kniess, Torsten; Caballero, Julio

    2012-02-01

    Semaxanib (SU5416) and 3-[4'-fluorobenzylidene]indolin-2-one (SU5205) are structurally similar drugs that are able to inhibit vascular endothelial growth factor receptor-2 (VEGFR2), but the former is 87 times more effective than the latter. Previously, SU5205 was used as a radiolabelled inhibitor (as surrogate for SU5416) and a radiotracer for positron emission tomography (PET) imaging, but the compound exhibited poor stability and only a moderate IC(50) toward VEGFR2. In the current work, the relationship between the structure and activity of these drugs as VEGFR2 inhibitors was studied using 3D-QSAR, docking and molecular dynamics (MD) simulations. First, comparative molecular field analysis (CoMFA) was performed using 48 2-indolinone derivatives and their VEGFR2 inhibitory activities. The best CoMFA model was carried out over a training set including 40 compounds, and it included steric and electrostatic fields. In addition, this model gave satisfactory cross-validation results and adequately predicted 8 compounds contained in the test set. The plots of the CoMFA fields could explain the structural differences between semaxanib and SU5205. Docking and molecular dynamics simulations showed that both molecules have the same orientation and dynamics inside the VEGFR2 active site. However, the hydrophobic pocket of VEGFR2 was more exposed to the solvent media when it was complexed with SU5205. An energetic analysis, including Embrace and MM-GBSA calculations, revealed that the potency of ligand binding is governed by van der Waals contacts. PMID:22070999

  16. Using genetic algorithms to optimize an active sensor network on a stiffened aerospace panel with 3D scanning laser vibrometry data

    NASA Astrophysics Data System (ADS)

    Marks, R.; Clarke, A.; Featherston, C.; Kawashita, L.; Paget, C.; Pullin, R.

    2015-07-01

    With the increasing complexity of aircraft structures and materials there is an essential need to continually monitor the structure for damage. This also drives the requirement for optimizing the location of sensors for damage detection to ensure full damage detection coverage of the structure whilst minimizing the number of sensors required, hence reducing costs, weight and data processing. An experiment was carried out to investigate the optimal sensor locations of an active sensor network for detecting adhesive disbonds of a stiffened panel. A piezoelectric transducer was coupled to two different stiffened aluminium panels; one healthy and one with a 25.4mm long disbond. The transducer was positioned at five individual locations to assess the effectiveness of damage detection at different transmission locations. One excitation frequency of 100kHz was used for this study. The panels were scanned with a 3D scanning laser vibrometer which represented a network of ‘ideal’ receiving transducers. The responses measured on the disbonded panel were cross- correlated with those measured on the healthy panel at a large number of potential sensor locations. This generated a cost surface which a genetic algorithm could interrogate in order to find the optimal sensor locations for a given size of sensor network. Probabilistic techniques were used to consider multiple disbond location scenarios, in order to optimise the sensor network for maximum probability of detection across a range of disbond locations.

  17. From 2-D CuO nanosheets to 3-D hollow nanospheres: interface-assisted synthesis, surface photovoltage properties and photocatalytic activity

    SciTech Connect

    Zhu Jun; Qian Xuefeng

    2010-07-15

    CuO hierarchical hollow nanostructures, assembled by nanosheets, were successfully prepared in n-octanol/aqueous liquid system through a microwave approach. Controlled experiments revealed that both bubble and interface play key roles in determining the self-assembly process of CuO hierarchical hollow nanostructures, and the morphology/size of building blocks and final products could be readily tuned by adjusting reaction parameters. Furthermore, a self-assembly mechanism of aggregation-then-growth process through bubble template was proposed for the formation of the hollow hierarchical architectures. Photocatalytic performance evidenced that the obtained CuO hierarchical hollow nanostructures possessed superior photocatalytic efficiency on RhB than that of non-hollow nanostructures, which could be easily demonstrated by SPS response about the separation and recombination situation of photogenerated charges. - Graphical abstract: From 2-D CuO nanosheets to 3-D hollow nanospheres: interface-assisted synthesis, surface photovoltage properties and photocatalytic activity. Various CuO architectures with different morphologies and sizes, including hierarchical hollow nanostructures were prepared through a synergic bubble-template and interface-assisted approach.

  18. No more active galactic nuclei in clumpy disks than in smooth galaxies at z ∼ 2 in CANDELS/3D-HST

    SciTech Connect

    Trump, Jonathan R.; Luo, Bin; Brandt, W. N.; Barro, Guillermo; Guo, Yicheng; Koo, David C.; Faber, S. M.; Brammer, Gabriel B.; Ferguson, Henry C.; Grogin, Norman A.; Kartaltepe, Jeyhan; Koekemoer, Anton M.; Bell, Eric F.; Dekel, Avishai; Hopkins, Philip F.; Kocevski, Dale D.; McIntosh, Daniel H.; Momcheva, Ivelina; and others

    2014-10-01

    We use CANDELS imaging, 3D-HST spectroscopy, and Chandra X-ray data to investigate if active galactic nuclei (AGNs) are preferentially fueled by violent disk instabilities funneling gas into galaxy centers at 1.3 < z < 2.4. We select galaxies undergoing gravitational instabilities using the number of clumps and degree of patchiness as proxies. The CANDELS visual classification system is used to identify 44 clumpy disk galaxies, along with mass-matched comparison samples of smooth and intermediate morphology galaxies. We note that despite being mass-matched and having similar star formation rates, the smoother galaxies tend to be smaller disks with more prominent bulges compared to the clumpy galaxies. The lack of smooth extended disks is probably a general feature of the z ∼ 2 galaxy population, and means we cannot directly compare with the clumpy and smooth extended disks observed at lower redshift. We find that z ∼ 2 clumpy galaxies have slightly enhanced AGN fractions selected by integrated line ratios (in the mass-excitation method), but the spatially resolved line ratios indicate this is likely due to extended phenomena rather than nuclear AGNs. Meanwhile, the X-ray data show that clumpy, smooth, and intermediate galaxies have nearly indistinguishable AGN fractions derived from both individual detections and stacked non-detections. The data demonstrate that AGN fueling modes at z ∼ 1.85—whether violent disk instabilities or secular processes—are as efficient in smooth galaxies as they are in clumpy galaxies.

  19. The 3D plant canopy radiative transfer analysis in an Alaskan black spruce forest: the characteristics of fraction of absorbed photosynthetically active radiation in the heterogeneous landscape

    NASA Astrophysics Data System (ADS)

    Kobayashi, H.; Suzuki, R.; Nagai, S.; Nakai, T.; Kim, Y.

    2012-12-01

    Over the last couple of decades, the three dimensional plant canopy radiative transfer models have been developed, improved and used for the retrievals of biophysical variables of vegetative surface. Fraction of absorbed photosynthetically active radiation (FAPAR) by plant canopy, a similar variable to heating rate in the atmosphere, is one of the important biophysical variables to infer the terrestrial plant canopy photosynthesis. FAPAR can be estimated by the radiative transfer model inversion or the empirical relationships between FAPAR and vegetation indices such as normalized difference vegetation index (NDVI). To date, some global FAPAR products are publicly available. These products are estimated from the moderate resolution satellites such as MODIS and SPOT-VEGETATION. One may apply the similar FAPAR algorithms to higher spatial resolution satellites if the ecosystem structures are horizontally homogeneous, which means that the adjacent satellite pixels have a similar spectral properties. If the vegetation surface is highly heterogeneous, "domain average FAPAR", which assumes no net horizontal radiation fluxes, can be unrealistically high (more than 1). In this presentation, we analyzed the characteristics of FAPAR in a heterogeneous landscape. As a case study, we selected our study site in a sparse black spruce forest in Alaska. We conducted the field campaigns to measure forest structural and optical properties that are used in the radiative transfer simulation. We used a 3D radiative transfer, FLiES (Kobayashi, H. and H. Iwabuchi (2008), A coupled 1-D atmosphere and 3-D canopy radiative transfer model for canopy reflectance, light environment, and photosynthesis simulation in a heterogeneous landscape, Remote Sensing of Environment, 112, 173-185) to create a high resolution simulated spectral reflectance and FAPAR images over the course of the growing season. From the analysis, we show (1) FAPAR with no net horizontal fluxes assumption can be higher than

  20. Pharmacologically active microcarriers associated with thermosensitive hydrogel as a growth factor releasing biomimetic 3D scaffold for cardiac tissue-engineering.

    PubMed

    Karam, Jean-Pierre; Muscari, Claudio; Sindji, Laurence; Bastiat, Guillaume; Bonafè, Francesca; Venier-Julienne, Marie-Claire; Montero-Menei, N Claudia

    2014-10-28

    The challenge of tissue engineering of the infarcted heart is how to improve stem cell engraftment, survival, homing, and differentiation for myocardial repair. We here propose to integrate human adipose-derived stem cells (ADSCs) and pharmacologically active microcarriers (PAMs), a three-dimensional (3D) carrier of cells and growth factors, into an injectable hydrogel (HG), to obtain a system that stimulates the survival and/or differentiation of the grafted cells toward a cardiac phenotype. PAMs are biodegradable and non-cytotoxic poly(lactic-co-glycolic acid) (PLGA) microspheres conveying cells on their 3D surface that deliver continuously and in a controlled manner a growth factor (GF) acting on the transported cells and on the microenvironment to improve engraftment. The choice of the appropriate GF and its protection during the formulation process and delivery are essential. In this study two GFs, hepatocyte growth factor (HGF) and insulin-like growth factor (IGF-1), have been encapsulated under a solid state in order to limit their interaction with the polymer and conserve their integrity. GF precipitation conditions and release profile from PAMs have been first investigated before combining them to ADSCs. The released IGF-1 and HGF induced the protein synthesis of cardiac differentiation markers GATA4, Nkx2.5, cTnI and CX43 after 1week in vitro. Moreover, the GFs accelerated cell cycle progression, as suggested by the increased expression of Cyclin D1 mRNA and the widespread distribution of Ki67 protein. Integrating PAMs within the thermosensitive P407 hydrogel increased their elastic properties but decreased the transcription of most cardiac markers. In contrast, CX43 expression increased in ADSC-PAM-GF complexes embedded within the hydrogel compared to the ADSCs cultured alone in the absence of P407. These results suggest that particulate scaffolds releasing HGF and IGF-1 may be beneficial for applications in tissue-engineering strategies for myocardial

  1. Comparison of osteoclastogenesis and resorption activity of human osteoclasts on tissue culture polystyrene and on natural extracellular bone matrix in 2D and 3D.

    PubMed

    Kleinhans, C; Schmid, F F; Schmid, F V; Kluger, P J

    2015-07-10

    Bone homeostasis is maintained by osteoblasts (bone formation) and osteoclasts (bone resorption). While there have been numerous studies investigating mesenchymal stem cells and their potential to differentiate into osteoblasts as well as their interaction with different bone substitute materials, there is only limited knowledge concerning in vitro generated osteoclasts. Due to the increasing development of degradable bone-grafting materials and the need of sophisticated in vitro test methods, it is essential to gain deeper insight into the process of osteoclastogenesis and the resorption functionality of human osteoclasts. Therefore, we focused on the comparison of osteoclastogenesis and resorption activity on tissue culture polystyrene (TCPS) and bovine extracellular bone matrices (BMs). Cortical bone slices were used as two-dimensional (2D) substrates, whereas a thermally treated cancellous bone matrix was used for three-dimensional (3D) experiments. We isolated primary human monocytes and induced osteoclastogenesis by medium supplementation. Subsequently, the expression of the vitronectin receptor (αVβ3) and cathepsin K as well as the characteristic actin formation on TCPS and the two BMs were examined. The cell area of human osteoclasts was analyzed on TCPS and on BMs, whereas significantly larger osteoclasts could be detected on BMs. Additionally, we compared the diameter of the sealing zones with the measured diameter of the resorption pits on the BMs and revealed similar diameters of the sealing zones and the resorption pits. We conclude that using TCPS as culture substrate does not affect the expression of osteoclast-specific markers. The analysis of resorption activity can successfully be conducted on cortical as well as on cancellous bone matrices. For new in vitro test systems concerning bone resorption, we suggest the establishment of a 2D assay for high throughput screening of new degradable bone substitute materials with osteoclasts. PMID:25562421

  2. Real-time monitoring of 3D cell culture using a 3D capacitance biosensor.

    PubMed

    Lee, Sun-Mi; Han, Nalae; Lee, Rimi; Choi, In-Hong; Park, Yong-Beom; Shin, Jeon-Soo; Yoo, Kyung-Hwa

    2016-03-15

    Three-dimensional (3D) cell cultures have recently received attention because they represent a more physiologically relevant environment compared to conventional two-dimensional (2D) cell cultures. However, 2D-based imaging techniques or cell sensors are insufficient for real-time monitoring of cellular behavior in 3D cell culture. Here, we report investigations conducted with a 3D capacitance cell sensor consisting of vertically aligned pairs of electrodes. When GFP-expressing human breast cancer cells (GFP-MCF-7) encapsulated in alginate hydrogel were cultured in a 3D cell culture system, cellular activities, such as cell proliferation and apoptosis at different heights, could be monitored non-invasively and in real-time by measuring the change in capacitance with the 3D capacitance sensor. Moreover, we were able to monitor cell migration of human mesenchymal stem cells (hMSCs) with our 3D capacitance sensor. PMID:26386332

  3. 3-D Art Tasks.

    ERIC Educational Resources Information Center

    Niswander, Virginia

    1983-01-01

    Perceptual motor dysfunctions may not allow children with learning and behavior problems to perform as most children do. A successful art activity for these children is construction using wood scraps. (SR)

  4. Earthquakes in Action: Incorporating Multimedia, Internet Resources, Large-scale Seismic Data, and 3-D Visualizations into Innovative Activities and Research Projects for Today's High School Students

    NASA Astrophysics Data System (ADS)

    Smith-Konter, B.; Jacobs, A.; Lawrence, K.; Kilb, D.

    2006-12-01

    The most effective means of communicating science to today's "high-tech" students is through the use of visually attractive and animated lessons, hands-on activities, and interactive Internet-based exercises. To address these needs, we have developed Earthquakes in Action, a summer high school enrichment course offered through the California State Summer School for Mathematics and Science (COSMOS) Program at the University of California, San Diego. The summer course consists of classroom lectures, lab experiments, and a final research project designed to foster geophysical innovations, technological inquiries, and effective scientific communication (http://topex.ucsd.edu/cosmos/earthquakes). Course content includes lessons on plate tectonics, seismic wave behavior, seismometer construction, fault characteristics, California seismicity, global seismic hazards, earthquake stress triggering, tsunami generation, and geodetic measurements of the Earth's crust. Students are introduced to these topics through lectures-made-fun using a range of multimedia, including computer animations, videos, and interactive 3-D visualizations. These lessons are further enforced through both hands-on lab experiments and computer-based exercises. Lab experiments included building hand-held seismometers, simulating the frictional behavior of faults using bricks and sandpaper, simulating tsunami generation in a mini-wave pool, and using the Internet to collect global earthquake data on a daily basis and map earthquake locations using a large classroom map. Students also use Internet resources like Google Earth and UNAVCO/EarthScope's Jules Verne Voyager Jr. interactive mapping tool to study Earth Science on a global scale. All computer-based exercises and experiments developed for Earthquakes in Action have been distributed to teachers participating in the 2006 Earthquake Education Workshop, hosted by the Visualization Center at Scripps Institution of Oceanography (http

  5. The Impact of 3D Volume-of-Interest Definition on Accuracy and Precision of Activity Estimation in Quantitative SPECT and Planar Processing Methods

    PubMed Central

    He, Bin; Frey, Eric C.

    2010-01-01

    Accurate and precise estimation of organ activities is essential for treatment planning in targeted radionuclide therapy. We have previously evaluated the impact of processing methodology, statistical noise, and variability in activity distribution and anatomy on the accuracy and precision of organ activity estimates obtained with quantitative SPECT (QSPECT), and planar (QPlanar) processing. Another important effect impacting the accuracy and precision of organ activity estimates is accuracy of and variability in the definition of organ regions of interest (ROI) or volumes of interest (VOI). The goal of this work was thus to systematically study the effects of VOI definition on the reliability of activity estimates. To this end, we performed Monte Carlo simulation studies using randomly perturbed and shifted VOIs to assess the impact on organ activity estimations. The 3D NCAT phantom was used with activities that modeled clinically observed 111In ibritumomab tiuxetan distributions. In order to study the errors resulting from misdefinitions due to manual segmentation errors, VOIs of the liver and left kidney were first manually defined. Each control point was then randomly perturbed to one of the nearest or next-nearest voxels in the same transaxial plane in three ways: with no, inward or outward directional bias, resulting in random perturbation, erosion or dilation, respectively of the VOIs. In order to study the errors resulting from the misregistration of VOIs, as would happen, e.g., in the case where the VOIs were defined using a misregistered anatomical image, the reconstructed SPECT images or projections were shifted by amounts ranging from −1 to 1 voxels in increments of 0.1 voxels in both the transaxial and axial directions. The activity estimates from the shifted reconstructions or projections were compared to those from the originals, and average errors were computed for the QSPECT and QPlanar methods, respectively. For misregistration, errors in organ

  6. Activation of Transcription Factor GAX and Concomitant Downregulation of IL-1β and ERK1/2 Modulate Vascular Smooth Muscle Cell Phenotype in 3D Fibrous Scaffolds.

    PubMed

    Lin, Shigang; Mequanint, Kibret

    2015-09-01

    Since vascular smooth muscle cells (VSMCs) display phenotypic plasticity in response to changing environmental cues, understanding the molecular mechanisms underlying the phenotypic modulation mediated by a three-dimensional (3D) scaffold is important to engineer functional vasculature. Following cell seeding into 3D scaffolds, the synthetic phenotype is desired to enable cells to expand rapidly and produce and assemble extracellular matrix components, but must revert to a quiescent contractile phenotype after tissue fabrication to impart the contractile properties found in native blood vessels. This study shows that 3D electrospun fibrous scaffolds regulate human coronary artery smooth muscle cells (HCASMCs) toward a more synthetic phenotype characterized by reduced contractile markers, such as smooth muscle alpha-actin and calponin. The reduction in contractile markers expression was mediated by endogenously expressed proinflammatory cytokine interleukin-1β (IL-1β). 3D topography transiently induces concomitant upregulation of IL-1β and MAPK ERK1/2 through nuclear factor-κB-dependent signaling pathway. An early burst of expression of IL-1β is essential for suppression of the homeobox transcription factor Gax and related cyclin-dependent kinase inhibitor p21(Cip1), which are key regulators for cells exiting from cell cycle. Our findings provide new insights for understanding signaling mechanisms of HCASMCs in electrospun 3D fibrous scaffolds, which have considerable value for application in vascular tissue engineering. PMID:26041434

  7. Gravity-induced stresses near a vertical cliff

    USGS Publications Warehouse

    Savage, W.Z.

    1993-01-01

    The exact solution for gravity-induced stresses beneath a vertical cliff presented here has application to the design of cut slopes in rock, compares favorably with published photoelastic and finite-element results for this problem, and satisfies the condition that shear and normal stresses vanish on the ground surface, except at the bottom corner where stress concentrations exist. The solution predicts that horizontal stresses are tensile away from the bottom of the cliff-effects caused by movement below the cliff in response to the gravity loading of the cliff. Also, it is shown that along the top of the cliff normal stresses reduce to those predicted for laterally constrained flat-lying topography. ?? 1993.

  8. Analysis of trabecular bone architectural changes induced by osteoarthritis in rabbit femur using 3D active shape model and digital topology

    NASA Astrophysics Data System (ADS)

    Saha, P. K.; Rajapakse, C. S.; Williams, D. S.; Duong, L.; Coimbra, A.

    2007-03-01

    Osteoarthritis (OA) is the most common chronic joint disease, which causes the cartilage between the bone joints to wear away, leading to pain and stiffness. Currently, progression of OA is monitored by measuring joint space width using x-ray or cartilage volume using MRI. However, OA affects all periarticular tissues, including cartilage and bone. It has been shown previously that in animal models of OA, trabecular bone (TB) architecture is particularly affected. Furthermore, relative changes in architecture are dependent on the depth of the TB region with respect to the bone surface and main direction of load on the bone. The purpose of this study was to develop a new method for accurately evaluating 3D architectural changes induced by OA in TB. Determining the TB test domain that represents the same anatomic region across different animals is crucial for studying disease etiology, progression and response to therapy. It also represents a major technical challenge in analyzing architectural changes. Here, we solve this problem using a new active shape model (ASM)-based approach. A new and effective semi-automatic landmark selection approach has been developed for rabbit distal femur surface that can easily be adopted for many other anatomical regions. It has been observed that, on average, a trained operator can complete the user interaction part of landmark specification process in less than 15 minutes for each bone data set. Digital topological analysis and fuzzy distance transform derived parameters are used for quantifying TB architecture. The method has been applied on micro-CT data of excised rabbit femur joints from anterior cruciate ligament transected (ACLT) (n = 6) and sham (n = 9) operated groups collected at two and two-to-eight week post-surgery, respectively. An ASM of the rabbit right distal femur has been generated from the sham group micro-CT data. The results suggest that, in conjunction with ASM, digital topological parameters are suitable for

  9. No More Active Galactic Nuclei in Clumpy Disks Than in Smooth Galaxies at z ~ 2 in CANDELS/3D-HST

    NASA Astrophysics Data System (ADS)

    Trump, Jonathan R.; Barro, Guillermo; Juneau, Stéphanie; Weiner, Benjamin J.; Luo, Bin; Brammer, Gabriel B.; Bell, Eric F.; Brandt, W. N.; Dekel, Avishai; Guo, Yicheng; Hopkins, Philip F.; Koo, David C.; Kocevski, Dale D.; McIntosh, Daniel H.; Momcheva, Ivelina; Faber, S. M.; Ferguson, Henry C.; Grogin, Norman A.; Kartaltepe, Jeyhan; Koekemoer, Anton M.; Lotz, Jennifer; Maseda, Michael; Mozena, Mark; Nandra, Kirpal; Rosario, David J.; Zeimann, Gregory R.

    2014-10-01

    We use CANDELS imaging, 3D-HST spectroscopy, and Chandra X-ray data to investigate if active galactic nuclei (AGNs) are preferentially fueled by violent disk instabilities funneling gas into galaxy centers at 1.3 < z < 2.4. We select galaxies undergoing gravitational instabilities using the number of clumps and degree of patchiness as proxies. The CANDELS visual classification system is used to identify 44 clumpy disk galaxies, along with mass-matched comparison samples of smooth and intermediate morphology galaxies. We note that despite being mass-matched and having similar star formation rates, the smoother galaxies tend to be smaller disks with more prominent bulges compared to the clumpy galaxies. The lack of smooth extended disks is probably a general feature of the z ~ 2 galaxy population, and means we cannot directly compare with the clumpy and smooth extended disks observed at lower redshift. We find that z ~ 2 clumpy galaxies have slightly enhanced AGN fractions selected by integrated line ratios (in the mass-excitation method), but the spatially resolved line ratios indicate this is likely due to extended phenomena rather than nuclear AGNs. Meanwhile, the X-ray data show that clumpy, smooth, and intermediate galaxies have nearly indistinguishable AGN fractions derived from both individual detections and stacked non-detections. The data demonstrate that AGN fueling modes at z ~ 1.85—whether violent disk instabilities or secular processes—are as efficient in smooth galaxies as they are in clumpy galaxies. Based on observations with the NASA/ESA Hubble Space Telescope, obtained at the Space Telescope Science Institute, which is operated by AURA Inc, under NASA contract NAS 5-26555.

  10. Biomolecule-assisted solvothermal synthesis of 3D hierarchical Cu2FeSnS4 microspheres with enhanced photocatalytic activity

    NASA Astrophysics Data System (ADS)

    Zhong, Jiasong; Wang, Qingyao; Chen, Daqin; Chen, Leifeng; Yu, Hua; Lu, Hongwei; Ji, Zhenguo

    2015-07-01

    In this work, we developed a novel environmentally friendly strategy toward solvothermal synthesis of 3D hierarchical Cu2FeSnS4 microspheres with the utilization of L-cystine, an amino acid, as a coordination agent and sulfur donor. The crystal structure, surface morphology and microstructure of the as-prepared products were investigated. The results showed that the 3D hierarchical Cu2FeSnS4 microspheres were composed of stannite nanosheets, and the average thickness of nanosheets was 100 nm. Moreover, the 3D hierarchical Cu2FeSnS4 exhibited high efficiency in the photodegradation of RhB under visible light illumination, suggesting a promising candidate for treatment of organic pollutants in waste water.

  11. Predicting coastal cliff erosion using a Bayesian probabilistic model

    USGS Publications Warehouse

    Hapke, C.; Plant, N.

    2010-01-01

    Regional coastal cliff retreat is difficult to model due to the episodic nature of failures and the along-shore variability of retreat events. There is a growing demand, however, for predictive models that can be used to forecast areas vulnerable to coastal erosion hazards. Increasingly, probabilistic models are being employed that require data sets of high temporal density to define the joint probability density function that relates forcing variables (e.g. wave conditions) and initial conditions (e.g. cliff geometry) to erosion events. In this study we use a multi-parameter Bayesian network to investigate correlations between key variables that control and influence variations in cliff retreat processes. The network uses Bayesian statistical methods to estimate event probabilities using existing observations. Within this framework, we forecast the spatial distribution of cliff retreat along two stretches of cliffed coast in Southern California. The input parameters are the height and slope of the cliff, a descriptor of material strength based on the dominant cliff-forming lithology, and the long-term cliff erosion rate that represents prior behavior. The model is forced using predicted wave impact hours. Results demonstrate that the Bayesian approach is well-suited to the forward modeling of coastal cliff retreat, with the correct outcomes forecast in 70-90% of the modeled transects. The model also performs well in identifying specific locations of high cliff erosion, thus providing a foundation for hazard mapping. This approach can be employed to predict cliff erosion at time-scales ranging from storm events to the impacts of sea-level rise at the century-scale. ?? 2010.

  12. Antiproliferative, DNA intercalation and redox cycling activities of dioxonaphtho[2,3-d]imidazolium analogs of YM155: A structure-activity relationship study.

    PubMed

    Ho, Si-Han Sherman; Sim, Mei-Yi; Yee, Wei-Loong Sherman; Yang, Tianming; Yuen, Shyi-Peng John; Go, Mei-Lin

    2015-11-01

    The anticancer agent YM155 is widely investigated as a specific survivin suppressant. More recently, YM155 was found to induce DNA damage and this has raised doubts as to whether survivin is its primary target. In an effort to assess the contribution of DNA damage to the anticancer activity of YM155, several analogs were prepared and evaluated for antiproliferative activity on malignant cells, participation in DNA intercalation and free radical generation by redox cycling. The intact positively charged scaffold was found to be essential for antiproliferative activity and intercalation but was less critical for redox cycling where the minimal requirement was a pared down bicyclic quinone. Side chain requirements at the N(1) and N(3) positions of the scaffold were more alike for redox cycling and intercalation than antiproliferative activity, underscoring yet again, the limited structural overlaps for these activities. Furthermore, antiproliferative activities were poorly correlated to DNA intercalation and redox cycling. Potent antiproliferative activity (IC50 9-23 nM), exceeding that of YM155, was found for a minimally substituted methyl analog AB7. Like YM155 and other dioxonaphthoimidazoliums, AB7 was a modest DNA intercalator but with weak redox cycling activity. Thus, the capacity of this scaffold to inflict direct DNA damage leading to cell death may not be significant and YM155 should not be routinely classified as a DNA damaging agent. PMID:26433618

  13. Acquisition of C3d-Binding Activity by De Novo Donor-Specific HLA Antibodies Correlates With Graft Loss in Nonsensitized Pediatric Kidney Recipients.

    PubMed

    Comoli, P; Cioni, M; Tagliamacco, A; Quartuccio, G; Innocente, A; Fontana, I; Trivelli, A; Magnasco, A; Nocco, A; Klersy, C; Rubert, L; Ramondetta, M; Zecca, M; Garibotto, G; Ghiggeri, G M; Cardillo, M; Nocera, A; Ginevri, F

    2016-07-01

    Alloantibody-mediated graft injury is a major cause of kidney dysfunction and loss. The complement-binding ability of de novo donor-specific antibodies (dnDSAs) has been suggested as a prognostic tool to stratify patients for clinical risk. In this study, we analyzed posttransplant kinetics of complement-fixing dnDSAs and their role in antibody-mediated rejection development and graft loss. A total of 114 pediatric nonsensitized recipients of first kidney allograft were periodically monitored for dnDSAs using flow bead assays, followed by C3d and C1q assay in case of positivity. Overall, 39 patients developed dnDSAs, which were C1q(+) and C3d(+) in 25 and nine patients, respectively. At follow-up, progressive acquisition over time of dnDSA C1q and C3d binding ability, within the same antigenic specificity, was observed, paralleled by an increase in mean fluorescence intensity that correlated with clinical outcome. C3d-fixing dnDSAs were better fit to stratify graft loss risk when the different dnDSA categories were evaluated in combined models because the 10-year graft survival probability was lower in patients with C3d-binding dnDSA than in those without dnDSAs or with C1q(+) /C3d(-) or non-complement-binding dnDSAs (40% vs. 94%, 100%, and 100%, respectively). Based on the kinetics profile, we favor dnDSA removal or modulation at first confirmed positivity, with treatment intensification guided by dnDSA biological characteristics. PMID:26725780

  14. 77 FR 47121 - Calvert Cliffs Nuclear Power Plant, LLC; Calvert Cliffs Nuclear Power Plant, Units 1 and 2...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-07

    ... Consideration (73 FR 17148; March 31, 2008), states that ``Plant emergencies are extraordinary circumstances... From the Federal Register Online via the Government Publishing Office NUCLEAR REGULATORY COMMISSION Calvert Cliffs Nuclear Power Plant, LLC; Calvert Cliffs Nuclear Power Plant, Units 1 and...

  15. 3D Spectroscopy in Astronomy

    NASA Astrophysics Data System (ADS)

    Mediavilla, Evencio; Arribas, Santiago; Roth, Martin; Cepa-Nogué, Jordi; Sánchez, Francisco

    2011-09-01

    Preface; Acknowledgements; 1. Introductory review and technical approaches Martin M. Roth; 2. Observational procedures and data reduction James E. H. Turner; 3. 3D Spectroscopy instrumentation M. A. Bershady; 4. Analysis of 3D data Pierre Ferruit; 5. Science motivation for IFS and galactic studies F. Eisenhauer; 6. Extragalactic studies and future IFS science Luis Colina; 7. Tutorials: how to handle 3D spectroscopy data Sebastian F. Sánchez, Begona García-Lorenzo and Arlette Pécontal-Rousset.

  16. 3D Elevation Program—Virtual USA in 3D

    USGS Publications Warehouse

    Lukas, Vicki; Stoker, J.M.

    2016-01-01

    The U.S. Geological Survey (USGS) 3D Elevation Program (3DEP) uses a laser system called ‘lidar’ (light detection and ranging) to create a virtual reality map of the Nation that is very accurate. 3D maps have many uses with new uses being discovered all the time.  

  17. 3D Printing for Tissue Engineering

    PubMed Central

    Jia, Jia; Yao, Hai; Mei, Ying

    2016-01-01

    Tissue engineering aims to fabricate functional tissue for applications in regenerative medicine and drug testing. More recently, 3D printing has shown great promise in tissue fabrication with a structural control from micro- to macro-scale by using a layer-by-layer approach. Whether through scaffold-based or scaffold-free approaches, the standard for 3D printed tissue engineering constructs is to provide a biomimetic structural environment that facilitates tissue formation and promotes host tissue integration (e.g., cellular infiltration, vascularization, and active remodeling). This review will cover several approaches that have advanced the field of 3D printing through novel fabrication methods of tissue engineering constructs. It will also discuss the applications of synthetic and natural materials for 3D printing facilitated tissue fabrication. PMID:26869728

  18. Recent developments in DFD (depth-fused 3D) display and arc 3D display

    NASA Astrophysics Data System (ADS)

    Suyama, Shiro; Yamamoto, Hirotsugu

    2015-05-01

    We will report our recent developments in DFD (Depth-fused 3D) display and arc 3D display, both of which have smooth movement parallax. Firstly, fatigueless DFD display, composed of only two layered displays with a gap, has continuous perceived depth by changing luminance ratio between two images. Two new methods, called "Edge-based DFD display" and "Deep DFD display", have been proposed in order to solve two severe problems of viewing angle and perceived depth limitations. Edge-based DFD display, layered by original 2D image and its edge part with a gap, can expand the DFD viewing angle limitation both in 2D and 3D perception. Deep DFD display can enlarge the DFD image depth by modulating spatial frequencies of front and rear images. Secondly, Arc 3D display can provide floating 3D images behind or in front of the display by illuminating many arc-shaped directional scattering sources, for example, arcshaped scratches on a flat board. Curved Arc 3D display, composed of many directional scattering sources on a curved surface, can provide a peculiar 3D image, for example, a floating image in the cylindrical bottle. The new active device has been proposed for switching arc 3D images by using the tips of dual-frequency liquid-crystal prisms as directional scattering sources. Directional scattering can be switched on/off by changing liquid-crystal refractive index, resulting in switching of arc 3D image.

  19. Hierarchical fabrication of heterojunctioned SrTiO3/TiO2 nanotubes on 3D microporous Ti substrate with enhanced photocatalytic activity and adhesive strength

    NASA Astrophysics Data System (ADS)

    Zhou, Jie; Yin, Lu; Zha, Kang; Li, Huirong; Liu, Zhiyuan; Wang, Jianxin; Duan, Ke; Feng, Bo

    2016-03-01

    Recently, construction of three-dimensional (3D) architecture and design of heterostructure have been proved to be two important approaches for improving photocatalytic (PC) properties of TiO2-based catalysts. In this work, a 3D microporous surface on Ti substrate (MPT) was prepared by simple acid etching. Then, heterojunctioned SrTiO3/TiO2 nanotubes with dominant {001} facets of anatase TiO2were successfully fabricated on MPT by combining anodization with hydrothermal treatment. The 3D microporous-patterned SrTiO3/TiO2 nanotubes heterojunction shows significantly enhanced photo-current density and ∼200% improved PC effect in degradation of Rhodamine B owing to its higher specific surface area, stronger light-harvesting ability and positive heterojunction effect in comparison with TiO2 nanotubes formed on flat Ti substrate. Moreover, the 3D microporous structure on Ti substrate improved the adhesive strength between the nanotubes layer and Ti substrate, which can be ascribed to the effective release of internal stress. Therefore, this present strategy is expected to expand the application of TiO2-based catalysts in many fields which require excellent PC properties and mechanical stability.

  20. Modular 3-D Transport model

    EPA Science Inventory

    MT3D was first developed by Chunmiao Zheng in 1990 at S.S. Papadopulos & Associates, Inc. with partial support from the U.S. Environmental Protection Agency (USEPA). Starting in 1990, MT3D was released as a pubic domain code from the USEPA. Commercial versions with enhanced capab...

  1. Market study: 3-D eyetracker

    NASA Technical Reports Server (NTRS)

    1977-01-01

    A market study of a proposed version of a 3-D eyetracker for initial use at NASA's Ames Research Center was made. The commercialization potential of a simplified, less expensive 3-D eyetracker was ascertained. Primary focus on present and potential users of eyetrackers, as well as present and potential manufacturers has provided an effective means of analyzing the prospects for commercialization.

  2. LLNL-Earth3D

    2013-10-01

    Earth3D is a computer code designed to allow fast calculation of seismic rays and travel times through a 3D model of the Earth. LLNL is using this for earthquake location and global tomography efforts and such codes are of great interest to the Earth Science community.

  3. [3-D ultrasound in gastroenterology].

    PubMed

    Zoller, W G; Liess, H

    1994-06-01

    Three-dimensional (3D) sonography represents a development of noninvasive diagnostic imaging by real-time two-dimensional (2D) sonography. The use of transparent rotating scans, comparable to a block of glass, generates a 3D effect. The objective of the present study was to optimate 3D presentation of abdominal findings. Additional investigations were made with a new volumetric program to determine the volume of selected findings of the liver. The results were compared with the estimated volumes of 2D sonography and 2D computer tomography (CT). For the processing of 3D images, typical parameter constellations were found for the different findings, which facilitated processing of 3D images. In more than 75% of the cases examined we found an optimal 3D presentation of sonographic findings with respect to the evaluation criteria developed by us for the 3D imaging of processed data. Great differences were found for the estimated volumes of the findings of the liver concerning the three different techniques applied. 3D ultrasound represents a valuable method to judge morphological appearance in abdominal findings. The possibility of volumetric measurements enlarges its potential diagnostic significance. Further clinical investigations are necessary to find out if definite differentiation between benign and malign findings is possible. PMID:7919882

  4. 3D World Building System

    SciTech Connect

    2013-10-30

    This video provides an overview of the Sandia National Laboratories developed 3-D World Model Building capability that provides users with an immersive, texture rich 3-D model of their environment in minutes using a laptop and color and depth camera.

  5. 3D World Building System

    ScienceCinema

    None

    2014-02-26

    This video provides an overview of the Sandia National Laboratories developed 3-D World Model Building capability that provides users with an immersive, texture rich 3-D model of their environment in minutes using a laptop and color and depth camera.

  6. Euro3D Science Conference

    NASA Astrophysics Data System (ADS)

    Walsh, J. R.

    2004-02-01

    The Euro3D RTN is an EU funded Research Training Network to foster the exploitation of 3D spectroscopy in Europe. 3D spectroscopy is a general term for spectroscopy of an area of the sky and derives its name from its two spatial + one spectral dimensions. There are an increasing number of instruments which use integral field devices to achieve spectroscopy of an area of the sky, either using lens arrays, optical fibres or image slicers, to pack spectra of multiple pixels on the sky (``spaxels'') onto a 2D detector. On account of the large volume of data and the special methods required to reduce and analyse 3D data, there are only a few centres of expertise and these are mostly involved with instrument developments. There is a perceived lack of expertise in 3D spectroscopy spread though the astronomical community and its use in the armoury of the observational astronomer is viewed as being highly specialised. For precisely this reason the Euro3D RTN was proposed to train young researchers in this area and develop user tools to widen the experience with this particular type of data in Europe. The Euro3D RTN is coordinated by Martin M. Roth (Astrophysikalisches Institut Potsdam) and has been running since July 2002. The first Euro3D science conference was held in Cambridge, UK from 22 to 23 May 2003. The main emphasis of the conference was, in keeping with the RTN, to expose the work of the young post-docs who are funded by the RTN. In addition the team members from the eleven European institutes involved in Euro3D also presented instrumental and observational developments. The conference was organized by Andy Bunker and held at the Institute of Astronomy. There were over thirty participants and 26 talks covered the whole range of application of 3D techniques. The science ranged from Galactic planetary nebulae and globular clusters to kinematics of nearby galaxies out to objects at high redshift. Several talks were devoted to reporting recent observations with newly

  7. PLOT3D user's manual

    NASA Technical Reports Server (NTRS)

    Walatka, Pamela P.; Buning, Pieter G.; Pierce, Larry; Elson, Patricia A.

    1990-01-01

    PLOT3D is a computer graphics program designed to visualize the grids and solutions of computational fluid dynamics. Seventy-four functions are available. Versions are available for many systems. PLOT3D can handle multiple grids with a million or more grid points, and can produce varieties of model renderings, such as wireframe or flat shaded. Output from PLOT3D can be used in animation programs. The first part of this manual is a tutorial that takes the reader, keystroke by keystroke, through a PLOT3D session. The second part of the manual contains reference chapters, including the helpfile, data file formats, advice on changing PLOT3D, and sample command files.

  8. 3D printing in dentistry.

    PubMed

    Dawood, A; Marti Marti, B; Sauret-Jackson, V; Darwood, A

    2015-12-01

    3D printing has been hailed as a disruptive technology which will change manufacturing. Used in aerospace, defence, art and design, 3D printing is becoming a subject of great interest in surgery. The technology has a particular resonance with dentistry, and with advances in 3D imaging and modelling technologies such as cone beam computed tomography and intraoral scanning, and with the relatively long history of the use of CAD CAM technologies in dentistry, it will become of increasing importance. Uses of 3D printing include the production of drill guides for dental implants, the production of physical models for prosthodontics, orthodontics and surgery, the manufacture of dental, craniomaxillofacial and orthopaedic implants, and the fabrication of copings and frameworks for implant and dental restorations. This paper reviews the types of 3D printing technologies available and their various applications in dentistry and in maxillofacial surgery. PMID:26657435

  9. Erosion processes in molassic cliffs: the role of the rock surface temperature and atmospheric conditions

    NASA Astrophysics Data System (ADS)

    Carrea, Dario; Abellán, Antonio; Guerin, Antoine; Jaboyedoff, Michel; Voumard, Jérémie

    2014-05-01

    3D GB-LiDAR datasets in order to quantify the erosion rates and to correlate it with atmospheric conditions, aiming to analyze which parameters influence Molasse erosion process.

  10. A quantitative analysis of rock cliff erosion environments

    NASA Astrophysics Data System (ADS)

    Lim, M.; Rosser, N.; Petley, D. N.; Norman, E. C.; Barlow, J.

    2009-12-01

    The spatial patterns and temporal sequencing of failures from coastal rock cliffs are complex and typically generate weak correlations with environmental variables such as tidal inundation, wave energy, wind and rain. Consequently, understanding of rock cliff behaviour, its response to predicted changes in environmental forcing and, more specifically, the interaction between marine and climatic factors in influencing failure processes has remained limited. This work presents the results from the first attempt to characterise and quantify the conditions on coastal cliffs that lead to accelerated rates of material detachment. The rate of change in an 80 m high section of coastal rock cliffs has been surveyed annually with high-resolution terrestrial laser scanning (TLS). The rockfall data have been analysed according to a simplified source geology that exhibit distinct magnitude-frequency distributions relating to the dominance of particular failure types. An integrated network of sensors and instrumentation designed to reflect the lithological control on failure has been installed to examine both the distinction between prevailing conditions and those affecting the local cliff environment and the physical response of different rock types to micro-climatic processes. The monitoring system records near-surface rock strain, temperature, moisture and micro-seismic displacement in addition to air temperature, humidity, radiation, precipitation, water-level and three-dimensional wind characteristics. A characteristic environmental signal, unique to the cliff face material, has been identified that differs substantially from that experienced by the surrounding area; suggesting that established methods of meteorological and tidal data collection are insufficient and inappropriate to represent erosive processes. The interaction between thermo- and hydro-dynamics of the cliff environment and the physical response of the rock highlights the composite environmental effects

  11. PLOT3D/AMES, APOLLO UNIX VERSION USING GMR3D (WITH TURB3D)

    NASA Technical Reports Server (NTRS)

    Buning, P.

    1994-01-01

    PLOT3D is an interactive graphics program designed to help scientists visualize computational fluid dynamics (CFD) grids and solutions. Today, supercomputers and CFD algorithms can provide scientists with simulations of such highly complex phenomena that obtaining an understanding of the simulations has become a major problem. Tools which help the scientist visualize the simulations can be of tremendous aid. PLOT3D/AMES offers more functions and features, and has been adapted for more types of computers than any other CFD graphics program. Version 3.6b+ is supported for five computers and graphic libraries. Using PLOT3D, CFD physicists can view their computational models from any angle, observing the physics of problems and the quality of solutions. As an aid in designing aircraft, for example, PLOT3D's interactive computer graphics can show vortices, temperature, reverse flow, pressure, and dozens of other characteristics of air flow during flight. As critical areas become obvious, they can easily be studied more closely using a finer grid. PLOT3D is part of a computational fluid dynamics software cycle. First, a program such as 3DGRAPE (ARC-12620) helps the scientist generate computational grids to model an object and its surrounding space. Once the grids have been designed and parameters such as the angle of attack, Mach number, and Reynolds number have been specified, a "flow-solver" program such as INS3D (ARC-11794 or COS-10019) solves the system of equations governing fluid flow, usually on a supercomputer. Grids sometimes have as many as two million points, and the "flow-solver" produces a solution file which contains density, x- y- and z-momentum, and stagnation energy for each grid point. With such a solution file and a grid file containing up to 50 grids as input, PLOT3D can calculate and graphically display any one of 74 functions, including shock waves, surface pressure, velocity vectors, and particle traces. PLOT3D's 74 functions are organized into

  12. PLOT3D/AMES, APOLLO UNIX VERSION USING GMR3D (WITHOUT TURB3D)

    NASA Technical Reports Server (NTRS)

    Buning, P.

    1994-01-01

    PLOT3D is an interactive graphics program designed to help scientists visualize computational fluid dynamics (CFD) grids and solutions. Today, supercomputers and CFD algorithms can provide scientists with simulations of such highly complex phenomena that obtaining an understanding of the simulations has become a major problem. Tools which help the scientist visualize the simulations can be of tremendous aid. PLOT3D/AMES offers more functions and features, and has been adapted for more types of computers than any other CFD graphics program. Version 3.6b+ is supported for five computers and graphic libraries. Using PLOT3D, CFD physicists can view their computational models from any angle, observing the physics of problems and the quality of solutions. As an aid in designing aircraft, for example, PLOT3D's interactive computer graphics can show vortices, temperature, reverse flow, pressure, and dozens of other characteristics of air flow during flight. As critical areas become obvious, they can easily be studied more closely using a finer grid. PLOT3D is part of a computational fluid dynamics software cycle. First, a program such as 3DGRAPE (ARC-12620) helps the scientist generate computational grids to model an object and its surrounding space. Once the grids have been designed and parameters such as the angle of attack, Mach number, and Reynolds number have been specified, a "flow-solver" program such as INS3D (ARC-11794 or COS-10019) solves the system of equations governing fluid flow, usually on a supercomputer. Grids sometimes have as many as two million points, and the "flow-solver" produces a solution file which contains density, x- y- and z-momentum, and stagnation energy for each grid point. With such a solution file and a grid file containing up to 50 grids as input, PLOT3D can calculate and graphically display any one of 74 functions, including shock waves, surface pressure, velocity vectors, and particle traces. PLOT3D's 74 functions are organized into

  13. Wireless Rover Meets 3D Design and Product Development

    ERIC Educational Resources Information Center

    Deal, Walter F., III; Hsiung, Steve C.

    2016-01-01

    Today there are a number of 3D printing technologies that are low cost and within the budgets of middle and high school programs. Educational technology companies offer a variety of 3D printing technologies and parallel curriculum materials to enable technology and engineering teachers to easily add 3D learning activities to their programs.…

  14. Bioprinting of 3D hydrogels.

    PubMed

    Stanton, M M; Samitier, J; Sánchez, S

    2015-08-01

    Three-dimensional (3D) bioprinting has recently emerged as an extension of 3D material printing, by using biocompatible or cellular components to build structures in an additive, layer-by-layer methodology for encapsulation and culture of cells. These 3D systems allow for cell culture in a suspension for formation of highly organized tissue or controlled spatial orientation of cell environments. The in vitro 3D cellular environments simulate the complexity of an in vivo environment and natural extracellular matrices (ECM). This paper will focus on bioprinting utilizing hydrogels as 3D scaffolds. Hydrogels are advantageous for cell culture as they are highly permeable to cell culture media, nutrients, and waste products generated during metabolic cell processes. They have the ability to be fabricated in customized shapes with various material properties with dimensions at the micron scale. 3D hydrogels are a reliable method for biocompatible 3D printing and have applications in tissue engineering, drug screening, and organ on a chip models. PMID:26066320

  15. Unassisted 3D camera calibration

    NASA Astrophysics Data System (ADS)

    Atanassov, Kalin; Ramachandra, Vikas; Nash, James; Goma, Sergio R.

    2012-03-01

    With the rapid growth of 3D technology, 3D image capture has become a critical part of the 3D feature set on mobile phones. 3D image quality is affected by the scene geometry as well as on-the-device processing. An automatic 3D system usually assumes known camera poses accomplished by factory calibration using a special chart. In real life settings, pose parameters estimated by factory calibration can be negatively impacted by movements of the lens barrel due to shaking, focusing, or camera drop. If any of these factors displaces the optical axes of either or both cameras, vertical disparity might exceed the maximum tolerable margin and the 3D user may experience eye strain or headaches. To make 3D capture more practical, one needs to consider unassisted (on arbitrary scenes) calibration. In this paper, we propose an algorithm that relies on detection and matching of keypoints between left and right images. Frames containing erroneous matches, along with frames with insufficiently rich keypoint constellations, are detected and discarded. Roll, pitch yaw , and scale differences between left and right frames are then estimated. The algorithm performance is evaluated in terms of the remaining vertical disparity as compared to the maximum tolerable vertical disparity.

  16. Arena3D: visualization of biological networks in 3D

    PubMed Central

    Pavlopoulos, Georgios A; O'Donoghue, Seán I; Satagopam, Venkata P; Soldatos, Theodoros G; Pafilis, Evangelos; Schneider, Reinhard

    2008-01-01

    Background Complexity is a key problem when visualizing biological networks; as the number of entities increases, most graphical views become incomprehensible. Our goal is to enable many thousands of entities to be visualized meaningfully and with high performance. Results We present a new visualization tool, Arena3D, which introduces a new concept of staggered layers in 3D space. Related data – such as proteins, chemicals, or pathways – can be grouped onto separate layers and arranged via layout algorithms, such as Fruchterman-Reingold, distance geometry, and a novel hierarchical layout. Data on a layer can be clustered via k-means, affinity propagation, Markov clustering, neighbor joining, tree clustering, or UPGMA ('unweighted pair-group method with arithmetic mean'). A simple input format defines the name and URL for each node, and defines connections or similarity scores between pairs of nodes. The use of Arena3D is illustrated with datasets related to Huntington's disease. Conclusion Arena3D is a user friendly visualization tool that is able to visualize biological or any other network in 3D space. It is free for academic use and runs on any platform. It can be downloaded or lunched directly from . Java3D library and Java 1.5 need to be pre-installed for the software to run. PMID:19040715

  17. PLOT3D Export Tool for Tecplot

    NASA Technical Reports Server (NTRS)

    Alter, Stephen

    2010-01-01

    The PLOT3D export tool for Tecplot solves the problem of modified data being impossible to output for use by another computational science solver. The PLOT3D Exporter add-on enables the use of the most commonly available visualization tools to engineers for output of a standard format. The exportation of PLOT3D data from Tecplot has far reaching effects because it allows for grid and solution manipulation within a graphical user interface (GUI) that is easily customized with macro language-based and user-developed GUIs. The add-on also enables the use of Tecplot as an interpolation tool for solution conversion between different grids of different types. This one add-on enhances the functionality of Tecplot so significantly, it offers the ability to incorporate Tecplot into a general suite of tools for computational science applications as a 3D graphics engine for visualization of all data. Within the PLOT3D Export Add-on are several functions that enhance the operations and effectiveness of the add-on. Unlike Tecplot output functions, the PLOT3D Export Add-on enables the use of the zone selection dialog in Tecplot to choose which zones are to be written by offering three distinct options - output of active, inactive, or all zones (grid blocks). As the user modifies the zones to output with the zone selection dialog, the zones to be written are similarly updated. This enables the use of Tecplot to create multiple configurations of a geometry being analyzed. For example, if an aircraft is loaded with multiple deflections of flaps, by activating and deactivating different zones for a specific flap setting, new specific configurations of that aircraft can be easily generated by only writing out specific zones. Thus, if ten flap settings are loaded into Tecplot, the PLOT3D Export software can output ten different configurations, one for each flap setting.

  18. Analysis of a creeping marls event in the coastal cliffs of Bessin, Basse-Normandie, France

    NASA Astrophysics Data System (ADS)

    Vioget, Alizée; Michoud, Clément; Jaboyedoff, Michel; Maquaire, Olivier; Costa, Stéphane; Davidson, Robert; Derron, Marc-Henri

    2015-04-01

    The cliffs' retreat is a major issue for the management of coastal territories. Two coastal areas in "Calvados" and "Pays de Caux", French Normandy, are studied. The Bessin cliff is about 4.3 km long and lies between the World War II artillery batteries of Longues-sur-Mer and Arromanches-les-Bains. On the coastline, the cliff's height varies between 10 and 75 meters above sea level. The site's lithology is mainly composed by two formations: the Bessin limestones lie on top of the Port marls, which act as an aquitard. More or less important water outflows are therefore observed at the contact between the marls and the limestone. For this communication, we aim to focus on a complex landslide that happened in May 2013 near Cape Manvieux, estimating volumes and modelling the landslide kinematics. For that purpose, some field observations and measurement have been made in order to make a realistic profile and to understand the steps which lead to this 27 m high and 110 m wide event. In addition, a terrestrial LiDAR (Optech Ilris3D) acquisition of the instability was performed in July 2013 and is compared with the Litto3D (the continued DEM over land and see) acquired in 2011 by the IGN. This comparison shows a maximum cliffs' retreat of about 27 m and 30'000 m3 and a deposit accumulation of about 8 m height. In addition, a limestone rock column of 2'000 m3 and 18 m height within the toppled deposits could still collapse in a short time. Up to now, these site-specific investigations, set in the context of instabilities within the entire study area, let us suppose that the current state of the instability was created by multiple successive events. The landslide could hence be caused by a complex mix of creeping marls conditioned by its water content and pressure induced by overlying formations and toppling of limestone destabilised by the formation of back subvertical crack due to limestone exhumation debuttressing.

  19. Fdf in US3D

    NASA Astrophysics Data System (ADS)

    Otis, Collin; Ferrero, Pietro; Candler, Graham; Givi, Peyman

    2013-11-01

    The scalar filtered mass density function (SFMDF) methodology is implemented into the computer code US3D. This is an unstructured Eulerian finite volume hydrodynamic solver and has proven very effective for simulation of compressible turbulent flows. The resulting SFMDF-US3D code is employed for large eddy simulation (LES) on unstructured meshes. Simulations are conducted of subsonic and supersonic flows under non-reacting and reacting conditions. The consistency and the accuracy of the simulated results are assessed along with appraisal of the overall performance of the methodology. The SFMDF-US3D is now capable of simulating high speed flows in complex configurations.

  20. 3D optical measuring technologies and systems

    NASA Astrophysics Data System (ADS)

    Chugui, Yuri V.

    2005-02-01

    The results of the R & D activity of TDI SIE SB RAS in the field of the 3D optical measuring technologies and systems for noncontact 3D optical dimensional inspection applied to atomic and railway industry safety problems are presented. This activity includes investigations of diffraction phenomena on some 3D objects, using the original constructive calculation method. The efficient algorithms for precise determining the transverse and longitudinal sizes of 3D objects of constant thickness by diffraction method, peculiarities on formation of the shadow and images of the typical elements of the extended objects were suggested. Ensuring the safety of nuclear reactors and running trains as well as their high exploitation reliability requires a 100% noncontact precise inspection of geometrical parameters of their components. To solve this problem we have developed methods and produced the technical vision measuring systems LMM, CONTROL, PROFIL, and technologies for noncontact 3D dimensional inspection of grid spacers and fuel elements for the nuclear reactor VVER-1000 and VVER-440, as well as automatic laser diagnostic COMPLEX for noncontact inspection of geometric parameters of running freight car wheel pairs. The performances of these systems and the results of industrial testing are presented and discussed. The created devices are in pilot operation at Atomic and Railway Companies.

  1. The Illinois Natural Heritage Conservation Education Kit V. [Ecology and Management of Special Habitats: Dune, Cave, Cliff, Bluff, and Urban.

    ERIC Educational Resources Information Center

    Stone, Sally F.

    The ecology and management of special habitats is the theme of this instructional guide. It contains 24 activities designed to help teachers familiarize their students with dune, cave, cliff, bluff, and urban habitats in Illinois. Each activity (which is ready to be copied and given to students) includes an objective (called a mission) and…

  2. Wavefront construction in 3-D

    SciTech Connect

    Chilcoat, S.R. Hildebrand, S.T.

    1995-12-31

    Travel time computation in inhomogeneous media is essential for pre-stack Kirchhoff imaging in areas such as the sub-salt province in the Gulf of Mexico. The 2D algorithm published by Vinje, et al, has been extended to 3D to compute wavefronts in complicated inhomogeneous media. The 3D wavefront construction algorithm provides many advantages over conventional ray tracing and other methods of computing travel times in 3D. The algorithm dynamically maintains a reasonably consistent ray density without making a priori guesses at the number of rays to shoot. The determination of caustics in 3D is a straight forward geometric procedure. The wavefront algorithm also enables the computation of multi-valued travel time surfaces.

  3. Heterodyne 3D ghost imaging

    NASA Astrophysics Data System (ADS)

    Yang, Xu; Zhang, Yong; Yang, Chenghua; Xu, Lu; Wang, Qiang; Zhao, Yuan

    2016-06-01

    Conventional three dimensional (3D) ghost imaging measures range of target based on pulse fight time measurement method. Due to the limit of data acquisition system sampling rate, range resolution of the conventional 3D ghost imaging is usually low. In order to take off the effect of sampling rate to range resolution of 3D ghost imaging, a heterodyne 3D ghost imaging (HGI) system is presented in this study. The source of HGI is a continuous wave laser instead of pulse laser. Temporal correlation and spatial correlation of light are both utilized to obtain the range image of target. Through theory analysis and numerical simulations, it is demonstrated that HGI can obtain high range resolution image with low sampling rate.

  4. Combinatorial 3D Mechanical Metamaterials

    NASA Astrophysics Data System (ADS)

    Coulais, Corentin; Teomy, Eial; de Reus, Koen; Shokef, Yair; van Hecke, Martin

    2015-03-01

    We present a class of elastic structures which exhibit 3D-folding motion. Our structures consist of cubic lattices of anisotropic unit cells that can be tiled in a complex combinatorial fashion. We design and 3d-print this complex ordered mechanism, in which we combine elastic hinges and defects to tailor the mechanics of the material. Finally, we use this large design space to encode smart functionalities such as surface patterning and multistability.

  5. Synthesis, structures, electrochemical studies and antioxidant activity of 5-aryl-4-oxo-3,4,5,8-tetrahydropyrido[2,3-d]pyrimidine-7-carboxylic acids

    NASA Astrophysics Data System (ADS)

    Quiroga, Jairo; Romo, Pablo E.; Ortiz, Alejandro; Isaza, José Hipólito; Insuasty, Braulio; Abonia, Rodrigo; Nogueras, Manuel; Cobo, Justo

    2016-09-01

    The synthesis of 5-aryl-4-oxo-3,4,5,8-tetrahydropyrido[2,3-d]pyrimidine-7-carboxylic acids 3 from the reaction of 6-aminopyrimidines 1 with arylidene derivatives of pyruvic acid 2 under microwave and ultrasound irradiation is described. The orientation of cyclization process was determined by NMR measurements. The methodology provides advantages such as high yields and friendly to the environment without the use of solvents. The antioxidant properties, DPPH free radical scavenging, ORAC, and anodic potential oxidation of the new pyridopyrimidines were studied.

  6. Resistivity tomography of Pointe du Hoc cliffs for stability assessment

    NASA Astrophysics Data System (ADS)

    Udphuay, S.; Everett, M. E.; Warden, R.

    2008-12-01

    Pointe du Hoc WWII battlefield overlooking the English Channel in western Normandy, France, is an important cultural resource, being an integral component of the June 6 1944 D-Day invasion. Two major buildings, the forward observation post (OP) and Col. Rudder's command post (RCP), are now perched perilously close to the cliff's edge owing to six decades of cliff retreat. Geophysical surveys were carried out in March 2008 to investigate the risk of cliff failure and to inform possible geotechnical remediation strategies with a final goal toward re-opening the observation post that is now closed to visitors. The geophysical surveying is accomplished by high-resolution resistivity tomography, conducted in extreme topography and in the midst of dense cultural clutter. The results of the OP tomography indicate that the highest mass movement hazard is associated with the marine caverns at the base of the cliff at the point of strongest wave attack. These caverns occupy the future site of a sea arch which will threaten the OP building. There is a high probability of a soil wedge failure on the east facing cliff edge close to the OP building. Such a failure could damage or destroy the building. The possibility of a sudden catastrophic failure along any one of these fractures cannot be ruled out. The greatest risk at the RCP site, which is under less immediate threat, is associated with soil wedge failures at the top of the cliffs.

  7. From 3D view to 3D print

    NASA Astrophysics Data System (ADS)

    Dima, M.; Farisato, G.; Bergomi, M.; Viotto, V.; Magrin, D.; Greggio, D.; Farinato, J.; Marafatto, L.; Ragazzoni, R.; Piazza, D.

    2014-08-01

    In the last few years 3D printing is getting more and more popular and used in many fields going from manufacturing to industrial design, architecture, medical support and aerospace. 3D printing is an evolution of bi-dimensional printing, which allows to obtain a solid object from a 3D model, realized with a 3D modelling software. The final product is obtained using an additive process, in which successive layers of material are laid down one over the other. A 3D printer allows to realize, in a simple way, very complex shapes, which would be quite difficult to be produced with dedicated conventional facilities. Thanks to the fact that the 3D printing is obtained superposing one layer to the others, it doesn't need any particular work flow and it is sufficient to simply draw the model and send it to print. Many different kinds of 3D printers exist based on the technology and material used for layer deposition. A common material used by the toner is ABS plastics, which is a light and rigid thermoplastic polymer, whose peculiar mechanical properties make it diffusely used in several fields, like pipes production and cars interiors manufacturing. I used this technology to create a 1:1 scale model of the telescope which is the hardware core of the space small mission CHEOPS (CHaracterising ExOPlanets Satellite) by ESA, which aims to characterize EXOplanets via transits observations. The telescope has a Ritchey-Chrétien configuration with a 30cm aperture and the launch is foreseen in 2017. In this paper, I present the different phases for the realization of such a model, focusing onto pros and cons of this kind of technology. For example, because of the finite printable volume (10×10×12 inches in the x, y and z directions respectively), it has been necessary to split the largest parts of the instrument in smaller components to be then reassembled and post-processed. A further issue is the resolution of the printed material, which is expressed in terms of layers

  8. Synthesis and biological activities of tricyclic conformationally restricted tetrahydropyrido annulated furo[2,3-d]pyrimidines as inhibitors of dihydrofolate reductases.

    PubMed

    Gangjee, A; Elzein, E; Queener, S F; McGuire, J J

    1998-04-23

    The synthesis of seven 2,4-diamino-5,6,7,8-tetrahydro-7-substituted pyrido[4',3':4,5]furo[2,3-d]pyrimidines 1-6 are reported as nonclassical antifolate inhibitors of dihydrofolate reductase (DHFR) and compound 7 as a classical antifolate inhibitor of tumor cells in culture. The compounds were designed as conformationally restricted analogues of trimetrexate. The synthesis was accomplished from the cyclocondensation of 3-bromo-4-piperidone with 2, 4-diamino-6-hydroxypyrimidine to afford regiospecifically 2, 4-diamino-5,6,7,8-tetrahydropyrido[4',3':4,5]furo[2, 3-d]pyrimidine-7-hydrobromide (16). This in turn was alkylated with the appropriate benzyl halide to afford the target compounds 1-6. The classical antifolate 7 utilized 4-(chloromethyl)benzoyl-l-glutamic acid diethyl ester (17) instead of the benzyl halide for alkylation, followed by saponification to afford 7. Compounds 1-6 showed moderate inhibitory potency against DHFR from Pneumocystis carinii, Toxoplasma gondii, Mycobacterium avium, and rat liver. The classical analogue 7 was 88-fold more potent against M. avium DHFR than against rat liver DHFR. The classical analogue was also inhibitory against the growth of tumor cells, CCRF-CEM, and FaDu, in culture. PMID:9554874

  9. YouDash3D: exploring stereoscopic 3D gaming for 3D movie theaters

    NASA Astrophysics Data System (ADS)

    Schild, Jonas; Seele, Sven; Masuch, Maic

    2012-03-01

    Along with the success of the digitally revived stereoscopic cinema, events beyond 3D movies become attractive for movie theater operators, i.e. interactive 3D games. In this paper, we present a case that explores possible challenges and solutions for interactive 3D games to be played by a movie theater audience. We analyze the setting and showcase current issues related to lighting and interaction. Our second focus is to provide gameplay mechanics that make special use of stereoscopy, especially depth-based game design. Based on these results, we present YouDash3D, a game prototype that explores public stereoscopic gameplay in a reduced kiosk setup. It features live 3D HD video stream of a professional stereo camera rig rendered in a real-time game scene. We use the effect to place the stereoscopic effigies of players into the digital game. The game showcases how stereoscopic vision can provide for a novel depth-based game mechanic. Projected trigger zones and distributed clusters of the audience video allow for easy adaptation to larger audiences and 3D movie theater gaming.

  10. Xenobiotic metabolism capacities of human skin in comparison with a 3D epidermis model and keratinocyte-based cell culture as in vitro alternatives for chemical testing: activating enzymes (Phase I).

    PubMed

    Götz, Christine; Pfeiffer, Roland; Tigges, Julia; Blatz, Veronika; Jäckh, Christine; Freytag, Eva-Maria; Fabian, Eric; Landsiedel, Robert; Merk, Hans F; Krutmann, Jean; Edwards, Robert J; Pease, Camilla; Goebel, Carsten; Hewitt, Nicola; Fritsche, Ellen

    2012-05-01

    Skin is important for the absorption and metabolism of exposed chemicals such as cosmetics or pharmaceuticals. The Seventh Amendment to the EU Cosmetics Directive prohibits the use of animals for cosmetic testing for certain endpoints, such as genotoxicity; therefore, there is an urgent need to understand the xenobiotic metabolizing capacities of human skin and to compare these activities with reconstructed 3D skin models developed to replace animal testing. We have measured Phase I enzyme activities of cytochrome P450 (CYP) and cyclooxygenase (COX) in ex vivo human skin, the 3D skin model EpiDerm™ (EPI-200), immortalized keratinocyte-based cell lines and primary normal human epidermal keratinocytes. Our data demonstrate that basal CYP enzyme activities are very low in whole human skin and EPI-200 as well as keratinocytes. In addition, activities in monolayer cells differed from organotypic tissues after induction. COX activity was similar in skin, EPI-200 and NHEK cells, but was significantly lower in immortalized keratinocytes. Hence, the 3D model EPI-200 might represent a more suitable model for dermatotoxicological studies. Altogether, these data help to better understand skin metabolism and expand the knowledge of in vitro alternatives used for dermatotoxicity testing. PMID:22509833

  11. Remote 3D Medical Consultation

    NASA Astrophysics Data System (ADS)

    Welch, Greg; Sonnenwald, Diane H.; Fuchs, Henry; Cairns, Bruce; Mayer-Patel, Ketan; Yang, Ruigang; State, Andrei; Towles, Herman; Ilie, Adrian; Krishnan, Srinivas; Söderholm, Hanna M.

    Two-dimensional (2D) video-based telemedical consultation has been explored widely in the past 15-20 years. Two issues that seem to arise in most relevant case studies are the difficulty associated with obtaining the desired 2D camera views, and poor depth perception. To address these problems we are exploring the use of a small array of cameras to synthesize a spatially continuous range of dynamic three-dimensional (3D) views of a remote environment and events. The 3D views can be sent across wired or wireless networks to remote viewers with fixed displays or mobile devices such as a personal digital assistant (PDA). The viewpoints could be specified manually or automatically via user head or PDA tracking, giving the remote viewer virtual head- or hand-slaved (PDA-based) remote cameras for mono or stereo viewing. We call this idea remote 3D medical consultation (3DMC). In this article we motivate and explain the vision for 3D medical consultation; we describe the relevant computer vision/graphics, display, and networking research; we present a proof-of-concept prototype system; and we present some early experimental results supporting the general hypothesis that 3D remote medical consultation could offer benefits over conventional 2D televideo.

  12. Speaking Volumes About 3-D

    NASA Technical Reports Server (NTRS)

    2002-01-01

    In 1999, Genex submitted a proposal to Stennis Space Center for a volumetric 3-D display technique that would provide multiple users with a 360-degree perspective to simultaneously view and analyze 3-D data. The futuristic capabilities of the VolumeViewer(R) have offered tremendous benefits to commercial users in the fields of medicine and surgery, air traffic control, pilot training and education, computer-aided design/computer-aided manufacturing, and military/battlefield management. The technology has also helped NASA to better analyze and assess the various data collected by its satellite and spacecraft sensors. Genex capitalized on its success with Stennis by introducing two separate products to the commercial market that incorporate key elements of the 3-D display technology designed under an SBIR contract. The company Rainbow 3D(R) imaging camera is a novel, three-dimensional surface profile measurement system that can obtain a full-frame 3-D image in less than 1 second. The third product is the 360-degree OmniEye(R) video system. Ideal for intrusion detection, surveillance, and situation management, this unique camera system offers a continuous, panoramic view of a scene in real time.

  13. 2D and 3D Shear-Wave Velocity Structure to >1 Km Depth from Ambient and Active Surface Waves: Three "Deep Remi" Case Studies

    NASA Astrophysics Data System (ADS)

    Louie, J. N.; Pancha, A.; Pullammanappallil, S. K.

    2014-12-01

    Refraction microtermor routinely assesses 1D and 2D velocity-depth profiles to shallow depths of approximately 100 m primarily for engineering applications. Estimation of both shallow and deep (>100 m) shear-velocity structure are key elements in the assessment of urban areas for potential earthquake ground shaking, damage, and the calibration of recorded ground motions. Three independent studies investigated the ability of the refraction microtremor technology to image deep velocity structure, to depths exceeding 1 km (Deep ReMi). In the first study, we were able to delineate basin thicknesses of up to 900 m and the deep-basin velocity structure beneath the Reno-area basin. Constraints on lateral velocity changes in 3D as well as on velocity profiles extended down to 1500 m, and show a possible fault offset. This deployment used 30 stand-alone wireless instruments mated to 4.5 Hz geophones, along each of five arrays 2.9 to 5.8 km long. Rayleigh-wave dispersion was clear at frequencies as low as 0.5 Hz using 120 sec ambient urban noise records. The results allowed construction of a 3D velocity model, vetted by agreement with gravity studies. In a second test, a 5.8 km array delimited the southern edge of the Tahoe Basin, with constraints from gravity. There, bedrock depth increased by 250 m in thickness over a distance of 1600 m, with definition of the velocity of the deeper basin sediments. The third study delineated the collapse region of an underground nuclear explosion within a thick sequence of volcanic extrusives, using a shear-wave minivibe in a radial direction, and horizontal geophones. Analysis showed the cavity extends to 620 m depth, with a width of 180 m and a height of 220 m. Our results demonstrate that deep velocity structure can be recovered using ambient noise. This technique offers the ability to define 2D and 3D structural representations essential for seismic hazard evaluation.

  14. Observations of historical sea cliff retreat rates exceed long-term estimates derived from cosmogenic 10Be

    NASA Astrophysics Data System (ADS)

    Hurst, Martin D.; Rood, Dylan H.; Ellis, Michael A.; Anderson, Robert S.

    2015-04-01

    Historical observation of coastal retreat are limited to relatively short timescales (< 150 years), during which time humans may have modified the coastal environment. There is growing concern that rates of coastal change may be accelerated in the face of anticipated stormier climates and rising sea level, yet there is little knowledge of rates of coastal change prior to the relatively brief historical records. In order to make predictions about potential future coastal change it is important to establish baseline conditions averaged over longer time periods. Here we present analysis of sea cliff retreat throughout the Holocene averaged for chalk cliffs in south-east England using cosmogenic isotopes. We determine long-term rates of sea cliff erosion from 10Be measured from in-situ flint samples collected from three transects across coastal platforms in East Sussex. A numerical model of 10Be accumulation on an evolving coastal profile allows estimation of cliff retreat rate during the Holocene. The model accounts for variation in 10Be accumulation with tides and sea-level rise, and takes into account platform downwear and topographic shielding by adjacent cliffs. We find that cliff retreat rates during the Holocene were significantly slower (2-6 cm yr-1) than those derived from recent historical observations (15-25 cm yr-1). Modelled accumulation of 10Be requires retreat rates that increase rapidly in recent times, potentially reflecting human modification of the coastal sediment budget through construction of sea defences, flood defenses and aggregate extraction. Therefore knowledge of past human activity at the coastline may be important in anticipating future rates of coastal retreat.

  15. 3D-Printed Microfluidics.

    PubMed

    Au, Anthony K; Huynh, Wilson; Horowitz, Lisa F; Folch, Albert

    2016-03-14

    The advent of soft lithography allowed for an unprecedented expansion in the field of microfluidics. However, the vast majority of PDMS microfluidic devices are still made with extensive manual labor, are tethered to bulky control systems, and have cumbersome user interfaces, which all render commercialization difficult. On the other hand, 3D printing has begun to embrace the range of sizes and materials that appeal to the developers of microfluidic devices. Prior to fabrication, a design is digitally built as a detailed 3D CAD file. The design can be assembled in modules by remotely collaborating teams, and its mechanical and fluidic behavior can be simulated using finite-element modeling. As structures are created by adding materials without the need for etching or dissolution, processing is environmentally friendly and economically efficient. We predict that in the next few years, 3D printing will replace most PDMS and plastic molding techniques in academia. PMID:26854878

  16. Survey on Cosmogenic 26Al in Lewis Cliff Meteorites

    NASA Astrophysics Data System (ADS)

    Welten, K. C.; Alderliesten, C.; Lindner, L.

    1992-07-01

    INTRODUCTION: We have embarked upon a ^26Al gamma-ray survey of meteorites selected from about 2000 samples recently recovered from the Lewis Cliff Ice Fields (84 degrees 18'S/161 degrees 20'E). Due to its 705-ka half-life ^26Al can be used for estimating terrestrial ages and thus contribute to further characterization of Antarctic meteorites in addition to their classification and thermoluminescence (TL) properties. The ^26Al survey is also useful for identifying meteorites with unusual exposure histories, which merit additional measurements of cosmogenic radionuclides (by AMS) and noble gases. In addition, it provides clues on possible pairings. METHOD: Low-level gamma-ray spectroscopy is well suited for ^26Al survey work, since bulk meteorite samples can be measured routinely and nondestructively without any previous sample preparation. The required size of the samples (30-500 g) makes the method relatively independent of depth effects and compositional inhomogeneities. The use of a high-resolution GeLi detector also allows the determination of the natural ^40K activity and thus the K content of the samples, which can be used as an additional pairing criterion for ordinary chondrites. Also ^137Cs, a fall-out surface contamination [1], is simultaneously measured; low values may be characteristic for meteorites recently fallen or released from the ablating ice. For the detector an efficiency calibration curve has been made that adequately accounts for differences in size and shape of the meteorite samples. RESULTS and DISCUSSION: TERRESTRIAL AGES: So far, we have measured over 30 Lewis Cliff equilibrated H and L chondrites, collected from widely differing locations. Normalized to L-chondrite composition, the ^26Al contents range from 27 to 110 dpm/kg with peaks around 43 and 53 dpm/kg. This bimodal ^26Al distribution is reminiscent of that observed for Allan Hills ordinary chondrites [2]. Tentative terrestrial ages, calculated on the basis of ^26Al saturation

  17. Survey on Cosmogenic 26Al in Lewis Cliff Meteorites

    NASA Astrophysics Data System (ADS)

    Welten, K. C.; Alderliesten, C.; Lindner, L.

    1992-07-01

    INTRODUCTION: We have embarked upon a ^26Al gamma-ray survey of meteorites selected from about 2000 samples recently recovered from the Lewis Cliff Ice Fields (84 degrees 18'S/161 degrees 20'E). Due to its 705-ka half-life ^26Al can be used for estimating terrestrial ages and thus contribute to further characterization of Antarctic meteorites in addition to their classification and thermoluminescence (TL) properties. The ^26Al survey is also useful for identifying meteorites with unusual exposure histories, which merit additional measurements of cosmogenic radionuclides (by AMS) and noble gases. In addition, it provides clues on possible pairings. METHOD: Low-level gamma-ray spectroscopy is well suited for ^26Al survey work, since bulk meteorite samples can be measured routinely and nondestructively without any previous sample preparation. The required size of the samples (30-500 g) makes the method relatively independent of depth effects and compositional inhomogeneities. The use of a high-resolution GeLi detector also allows the determination of the natural ^40K activity and thus the K content of the samples, which can be used as an additional pairing criterion for ordinary chondrites. Also ^137Cs, a fall-out surface contamination [1], is simultaneously measured; low values may be characteristic for meteorites recently fallen or released from the ablating ice. For the detector an efficiency calibration curve has been made that adequately accounts for differences in size and shape of the meteorite samples. RESULTS and DISCUSSION: TERRESTRIAL AGES: So far, we have measured over 30 Lewis Cliff equilibrated H and L chondrites, collected from widely differing locations. Normalized to L-chondrite composition, the ^26Al contents range from 27 to 110 dpm/kg with peaks around 43 and 53 dpm/kg. This bimodal ^26Al distribution is reminiscent of that observed for Allan Hills ordinary chondrites [2]. Tentative terrestrial ages, calculated on the basis of ^26Al saturation

  18. Innovative radar products for the 3D, high-resolution and real-time monitoring of the convective activity in the airspace around airports

    NASA Astrophysics Data System (ADS)

    Tabary, P.; Bousquet, O.; Sénési, S.; Josse, P.

    2009-09-01

    Airports are recognized to become critical areas in the future given the expected doubling in air traffic by 2020. The increased density of aircrafts in the airport airspaces calls for improved systems and products to monitor in real-time potential hazards and thus meet the airport objectives in terms of safety and throughput. Among all meteorological hazards, convection is certainly the most impacting one. We describe here some innovative radar products that have recently been developed and tested at Météo France around the Paris airports. Those products rely on the French Doppler radar network consisting today of 24 elements with some of them being polarimetric. Reflectivity and Doppler volumetric data are concentrated from all 24 radar sites in real-time at the central level (Toulouse) where 3D Cartesian mosaics covering the entire French territory (i.e. a typical 1,000 by 1,000 km² area) are elaborated. The innovation with respect to what has been done previously is that the three components of the wind are retrieved by operational combination of the radial velocities. The final product, available in real-time every 15 minutes with a spatial resolution of 2.5 km horizontally and 500 m vertically, is a 3D grid giving the interpolated reflectivity and wind field (u, v and w) values. The 2.5 km resolution, arising from the fact that the retrieval is carried out every 15 minutes from radars typically spaced apart by 150 km, is not sufficient for airport airspace monitoring but is valuable for en-route monitoring. Its extension to the entire European space is foreseen. To address the specific needs in the airport areas, a downscaling technique has been proposed to merge the above-mentioned low-resolution 3D wind and reflectivity fields with the high resolution (5 minutes and 1 km²) 2D imagery of the Trappes radar that is the one that covers the Paris airports. The merging approach is based on the assumption that the Vertical Profile of Reflectivity (i.e. the

  19. Results From a Borehole Seismometer Array II: 3-D Mapping of an Active Geothermal Field at the Kilauea Lower Rift Zone

    NASA Astrophysics Data System (ADS)

    Shalev, E.; Kenedi, C. L.; Malin, P.

    2008-12-01

    The geothermal power plant in Puna, in southeastern Hawaii, is located in a section of the Kilauea Lower East Rift Zone that was resurfaced by lava flows as recently as 1955, 1960, and 1972. In 2006 a seismic array consisting of eight 3-component stations was installed around the geothermal field in Puna. The instrument depths range from 24 to 210 m. The shallower instruments have 2 Hz geophones and the deeper have 4.5 Hz geophones. 3-D tomographic analyses of P-wave velocity, S-wave velocity, and the Vp/Vs ratio show an area of very fast P-wave velocity at the relatively shallow depth of 2.5 km in the southern section of the field. The same area shows moderate S-wave velocity. This high P-wave velocity anomaly at the southern part of the geothermal field may indicate the presence of dense rock material usually found at greater depths.

  20. Selection, synthesis, and structure-activity relationship of tetrahydropyrido[4,3-d]pyrimidine-2,4-diones as human GnRH receptor antagonists.

    PubMed

    Lanier, Marion C; Feher, Miklos; Ashweek, Neil J; Loweth, Colin J; Rueter, Jaimie K; Slee, Deborah H; Williams, John P; Zhu, Yun-Fei; Sullivan, Susan K; Brown, Michael S

    2007-08-15

    The present article describes a selection of a new class of small molecule antagonists for the h-GnRH receptor, their preparation, and evaluation in vitro. Three computational methods were combined into a consensus score, to rank order virtual templates. The top 5% of templates were further evaluated in silico and assessed for novelty and synthetic accessibility. The tetrahydropyrido[4,3-d]pyrimidine-2,4-dione core was selected for synthesis and evaluated in vitro. Using an array approach for analog design and synthesis, we were able to drive the binding below 10nM for the h-GnRH receptor after two rounds of optimization. PMID:17561404

  1. Kilimanjaro ice cliff recession patterns derived from terrestrial photogrammetry

    NASA Astrophysics Data System (ADS)

    Winkler, Michael; Tad Pfeffer, W.; Hanke, Klaus; Cullen, Nicolas J.; Kaser, Georg

    2010-05-01

    Ice cliffs are intriguing features of glaciers around the world, but little is known about mechanisms of their formation and maintenance. Ice cliffs also characterize the plateau glaciers on Kilimanjaro, Tanzania (3° S, 37° E). Their heights range from 3 to more than 40 meters and they have at least persisted since the late 19th century when early explorers documented them. Snow accumulation is only possible on the flat parts of the plateau glaciers but not on the vertical or near-vertical cliffs. Dry calving due to ice dynamics is unlikely and ice ablation through sublimation and melt by far out weighs the negligible mass gain by deposition of water vapour. Consequently, as soon as the cliffs are established, they are forced to retreat and thus, the areal shrinkage of the glaciers on Africa's highest peak is closely linked to the existence of these cliffs. In order to extract climate change details from the glaciers on Kilimanjaro, the sensitivity of the ice cliffs to climate fluctuations must be understood. Strikingly, the cliffs are mainly either north- or south-facing which entails at this near-equatorial site that direct sunlight either always hits the cliff faces from dawn till dusk, or not at all. The examination of the annual insolation patterns at a 25m high, south-facing sample cliff shows that it is not hit by direct shortwave radiation from March to October. During this time not enough energy is available for melting and only sublimation occurs. From November to February the cliff is sunlit 12 hours a day and its surface temperature can reach 0° C. Melting sometimes occurs during some hours, although air temperature is almost always below freezing. Point measurements at the sample cliff reveal a 20-30 times faster retreat during the sunlit period because melting is a much more energy-efficient ablation process than sublimation. Repeat terrestrial photogrammetric surveys have been carried out at the transition dates from the shaded to the sunlit

  2. Metrological characterization of 3D imaging devices

    NASA Astrophysics Data System (ADS)

    Guidi, G.

    2013-04-01

    Manufacturers often express the performance of a 3D imaging device in various non-uniform ways for the lack of internationally recognized standard requirements for metrological parameters able to identify the capability of capturing a real scene. For this reason several national and international organizations in the last ten years have been developing protocols for verifying such performance. Ranging from VDI/VDE 2634, published by the Association of German Engineers and oriented to the world of mechanical 3D measurements (triangulation-based devices), to the ASTM technical committee E57, working also on laser systems based on direct range detection (TOF, Phase Shift, FM-CW, flash LADAR), this paper shows the state of the art about the characterization of active range devices, with special emphasis on measurement uncertainty, accuracy and resolution. Most of these protocols are based on special objects whose shape and size are certified with a known level of accuracy. By capturing the 3D shape of such objects with a range device, a comparison between the measured points and the theoretical shape they should represent is possible. The actual deviations can be directly analyzed or some derived parameters can be obtained (e.g. angles between planes, distances between barycenters of spheres rigidly connected, frequency domain parameters, etc.). This paper shows theoretical aspects and experimental results of some novel characterization methods applied to different categories of active 3D imaging devices based on both principles of triangulation and direct range detection.

  3. Low retreat rate sea cliff monitoring by photogrammetric methods

    NASA Astrophysics Data System (ADS)

    Marques, F.; Redweik, P.; Matildes, R.; Santos, L.

    2009-04-01

    Sea cliff evolution is dominated by the occurrence of mass movements of different types and sizes, being a considerable source of natural hazard in coastal areas. Hazard assessment requires the use of substantially complete inventories of cliff failures occurred in the past, which are usually produced by multi-temporal aerial photo based studies, where photogrammetric methods still combine the advantages of high accuracy and the possibility of monitoring periods of time in excess of half a century. However, the study of low retreat rate cliffs still involves problems, because parts of the cliff top contour remain unchanged during the monitoring period and retreat is restricted to the places where cliff failures have occurred. This situation creates problems related with the inherent difficulties of a multi-temporal processing of aerial photos (different flight directions and scales, insufficient ground control for older photos and poor radiometric quality) and requires the use of refined photogrammetric approaches to prevent the generation of false mass movements, which cannot be included in the inventories. Because cliff retreat is discontinuous in space and time, better sampling of the process requires the widening of the monitoring time window, by use of older aerial photos, which involve further problems as unavailable camera calibration and photo distortions. To address these problems, the sea cliffs of Burgau-Lagos coast (Southwest Algarve, Portugal) were studied using digital photogrammetry methods, which involved several aerotriangulation steps, generation of pseudo-camera data for the older aerial photos, stereo plotting of the cliff top, ridges and toe and automatic generation of digital terrain models. Inconsistencies in the results were solved by systematic stereo photo interpretation, supported by oblique aerial photos. This study enabled the detection and characterization of 137 cliff failures that occurred between 1947 and 2007 along the 13 km long

  4. Visualization of 3D Geological Data using COLLADA and KML

    NASA Astrophysics Data System (ADS)

    Choi, Yosoon; Um, Jeong-Gi; Park, Myong-Ho

    2013-04-01

    This study presents a method to visualize 3D geological data using COLLAborative Design Activity(COLLADA, an open standard XML schema for establishing interactive 3D applications) and Keyhole Markup Language(KML, the XML-based scripting language of Google Earth).We used COLLADA files to represent different 3D geological data such as borehole, fence section, surface-based 3D volume and 3D grid by triangle meshes(a set of triangles connected by their common edges or corners). The COLLADA files were imported into the 3D render window of Google Earth using KML codes. An application to the Grosmont formation in Alberta, Canada showed that the combination of COLLADA and KML enables Google Earth to visualize 3D geological structures and properties.

  5. SNL3dFace

    2007-07-20

    This software distribution contains MATLAB and C++ code to enable identity verification using 3D images that may or may not contain a texture component. The code is organized to support system performance testing and system capability demonstration through the proper configuration of the available user interface. Using specific algorithm parameters the face recognition system has been demonstrated to achieve a 96.6% verification rate (Pd) at 0.001 false alarm rate. The system computes robust facial featuresmore » of a 3D normalized face using Principal Component Analysis (PCA) and Fisher Linear Discriminant Analysis (FLDA). A 3D normalized face is obtained by alighning each face, represented by a set of XYZ coordinated, to a scaled reference face using the Iterative Closest Point (ICP) algorithm. The scaled reference face is then deformed to the input face using an iterative framework with parameters that control the deformed surface regulation an rate of deformation. A variety of options are available to control the information that is encoded by the PCA. Such options include the XYZ coordinates, the difference of each XYZ coordinates from the reference, the Z coordinate, the intensity/texture values, etc. In addition to PCA/FLDA feature projection this software supports feature matching to obtain similarity matrices for performance analysis. In addition, this software supports visualization of the STL, MRD, 2D normalized, and PCA synthetic representations in a 3D environment.« less

  6. Making Inexpensive 3-D Models

    ERIC Educational Resources Information Center

    Manos, Harry

    2016-01-01

    Visual aids are important to student learning, and they help make the teacher's job easier. Keeping with the "TPT" theme of "The Art, Craft, and Science of Physics Teaching," the purpose of this article is to show how teachers, lacking equipment and funds, can construct a durable 3-D model reference frame and a model gravity…

  7. SNL3dFace

    SciTech Connect

    Russ, Trina; Koch, Mark; Koudelka, Melissa; Peters, Ralph; Little, Charles; Boehnen, Chris; Peters, Tanya

    2007-07-20

    This software distribution contains MATLAB and C++ code to enable identity verification using 3D images that may or may not contain a texture component. The code is organized to support system performance testing and system capability demonstration through the proper configuration of the available user interface. Using specific algorithm parameters the face recognition system has been demonstrated to achieve a 96.6% verification rate (Pd) at 0.001 false alarm rate. The system computes robust facial features of a 3D normalized face using Principal Component Analysis (PCA) and Fisher Linear Discriminant Analysis (FLDA). A 3D normalized face is obtained by alighning each face, represented by a set of XYZ coordinated, to a scaled reference face using the Iterative Closest Point (ICP) algorithm. The scaled reference face is then deformed to the input face using an iterative framework with parameters that control the deformed surface regulation an rate of deformation. A variety of options are available to control the information that is encoded by the PCA. Such options include the XYZ coordinates, the difference of each XYZ coordinates from the reference, the Z coordinate, the intensity/texture values, etc. In addition to PCA/FLDA feature projection this software supports feature matching to obtain similarity matrices for performance analysis. In addition, this software supports visualization of the STL, MRD, 2D normalized, and PCA synthetic representations in a 3D environment.

  8. 3D Printing: Exploring Capabilities

    ERIC Educational Resources Information Center

    Samuels, Kyle; Flowers, Jim

    2015-01-01

    As 3D printers become more affordable, schools are using them in increasing numbers. They fit well with the emphasis on product design in technology and engineering education, allowing students to create high-fidelity physical models to see and test different iterations in their product designs. They may also help students to "think in three…

  9. Thermal monitoring of a granitic exfoliation sheet and cliff in Yosemite Valley, California (USA)

    NASA Astrophysics Data System (ADS)

    Guerin, Antoine; Matasci, Battista; Collins, Brian D.; Stock, Greg M.; Derron, Marc-Henri; Jaboyedoff, Michel

    2015-04-01

    100 stitched pictures and also draped on a TLS mesh to generate a 3D color model. This model shows the apparent temperatures measured according to position and surface orientation of the cliff. This rock wall has many recent rockfall scars with lighter colored rock surface; these scars appear as spots of lower temperature surrounded by warmer areas and may undergo increased stress related to the thermal variations. However, these first results must be verified by further testing using calibrated models to distinguish the effects of emissivity and thermal radiation. Subsequently, we plan to fix the thermal camera on a GigaPan EPIC Pro device to take sequences of panoramas during rock cooling and heating and to perform additional investigation on air and water propagation in fractured zones.

  10. Synthesis and Exploratory Catalysis of 3d Metals: Group-Transfer Reactions, and the Activation and Functionalization of Small Molecules Including Greenhouse Gases

    SciTech Connect

    Mindiola, Daniel J.

    2014-05-07

    Our work over the past three years has resulted in the development of electron rich and low-coordinate vanadium fragments, molecular nitrides of vanadium and parent imide systems of titanium, and the synthesis of phosphorus containing molecules of the 3d transition metal series. Likewise, with financial support from BES Division in DOE (DE-FG02-07ER15893), we now completed the full characterization of the first single molecular magnet (SMM) of Fe(III). We demonstrated that this monomeric form of Fe(III) has an unusual slow relaxation of the magnetization under zero applied field. To make matters more interesting, this system also undergoes a rare example of an intermediate to high-spin transition (an S = 3/2 to S = 5/2 transition). In 2010 we reported the synthesis of the first neutral and low-coordinate vanadium complexes having the terminal nitride functionality. We have now completed a full study to understand formation of the nitride ligand from the metastable azide precursor, and have also explored the reactivity of the nitride ligand in the context of incomplete and complete N-atom transfer. During the 2010-2013 period we also discovered a facile approach to assemble low-coordinate and low-valent vanadium(II) complexes and exploit their multielectron chemistry ranging from 1-3 electrons. Consequently, we can now access 3d ligand frameworks such as cyclo-P3 (and its corresponding radical anion), nitride radical anions and cations, low-coordinate vanadium oxo’s, and the first example of a vanadium thionitrosyl complex. A cis-divacant iron(IV) imido having some ligand centered radical has been also discovered, and we are in the process of elucidating its electronic structure (in particular the sign of zero field splitting and the origin of its magnitude), bonding and reactivity. We have also revisited some paramagnetic and classic metallocene compounds with S >1/2 ground states in order to understand their reactivity patterns and electronic structure. Lastly

  11. TACO3D. 3-D Finite Element Heat Transfer Code

    SciTech Connect

    Mason, W.E.

    1992-03-04

    TACO3D is a three-dimensional, finite-element program for heat transfer analysis. An extension of the two-dimensional TACO program, it can perform linear and nonlinear analyses and can be used to solve either transient or steady-state problems. The program accepts time-dependent or temperature-dependent material properties, and materials may be isotropic or orthotropic. A variety of time-dependent and temperature-dependent boundary conditions and loadings are available including temperature, flux, convection, and radiation boundary conditions and internal heat generation. Additional specialized features treat enclosure radiation, bulk nodes, and master/slave internal surface conditions (e.g., contact resistance). Data input via a free-field format is provided. A user subprogram feature allows for any type of functional representation of any independent variable. A profile (bandwidth) minimization option is available. The code is limited to implicit time integration for transient solutions. TACO3D has no general mesh generation capability. Rows of evenly-spaced nodes and rows of sequential elements may be generated, but the program relies on separate mesh generators for complex zoning. TACO3D does not have the ability to calculate view factors internally. Graphical representation of data in the form of time history and spatial plots is provided through links to the POSTACO and GRAPE postprocessor codes.

  12. 3D Printed Programmable Release Capsules.

    PubMed

    Gupta, Maneesh K; Meng, Fanben; Johnson, Blake N; Kong, Yong Lin; Tian, Limei; Yeh, Yao-Wen; Masters, Nina; Singamaneni, Srikanth; McAlpine, Michael C

    2015-08-12

    The development of methods for achieving precise spatiotemporal control over chemical and biomolecular gradients could enable significant advances in areas such as synthetic tissue engineering, biotic-abiotic interfaces, and bionanotechnology. Living organisms guide tissue development through highly orchestrated gradients of biomolecules that direct cell growth, migration, and differentiation. While numerous methods have been developed to manipulate and implement biomolecular gradients, integrating gradients into multiplexed, three-dimensional (3D) matrices remains a critical challenge. Here we present a method to 3D print stimuli-responsive core/shell capsules for programmable release of multiplexed gradients within hydrogel matrices. These capsules are composed of an aqueous core, which can be formulated to maintain the activity of payload biomolecules, and a poly(lactic-co-glycolic) acid (PLGA, an FDA approved polymer) shell. Importantly, the shell can be loaded with plasmonic gold nanorods (AuNRs), which permits selective rupturing of the capsule when irradiated with a laser wavelength specifically determined by the lengths of the nanorods. This precise control over space, time, and selectivity allows for the ability to pattern 2D and 3D multiplexed arrays of enzyme-loaded capsules along with tunable laser-triggered rupture and release of active enzymes into a hydrogel ambient. The advantages of this 3D printing-based method include (1) highly monodisperse capsules, (2) efficient encapsulation of biomolecular payloads, (3) precise spatial patterning of capsule arrays, (4) "on the fly" programmable reconfiguration of gradients, and (5) versatility for incorporation in hierarchical architectures. Indeed, 3D printing of programmable release capsules may represent a powerful new tool to enable spatiotemporal control over biomolecular gradients. PMID:26042472

  13. 3D Printed Programmable Release Capsules

    PubMed Central

    Gupta, Maneesh K.; Meng, Fanben; Johnson, Blake N.; Kong, Yong Lin; Tian, Limei; Yeh, Yao-Wen; Masters, Nina; Singamaneni, Srikanth; McAlpine, Michael C.

    2015-01-01

    The development of methods for achieving precise spatiotemporal control over chemical and biomolecular gradients could enable significant advances in areas such as synthetic tissue engineering, biotic–abiotic interfaces, and bionanotechnology. Living organisms guide tissue development through highly orchestrated gradients of biomolecules that direct cell growth, migration, and differentiation. While numerous methods have been developed to manipulate and implement biomolecular gradients, integrating gradients into multiplexed, three-dimensional (3D) matrices remains a critical challenge. Here we present a method to 3D print stimuli-responsive core/shell capsules for programmable release of multiplexed gradients within hydrogel matrices. These capsules are composed of an aqueous core, which can be formulated to maintain the activity of payload biomolecules, and a poly(lactic-co-glycolic) acid (PLGA, an FDA approved polymer) shell. Importantly, the shell can be loaded with plasmonic gold nanorods (AuNRs), which permits selective rupturing of the capsule when irradiated with a laser wavelength specifically determined by the lengths of the nanorods. This precise control over space, time, and selectivity allows for the ability to pattern 2D and 3D multiplexed arrays of enzyme-loaded capsules along with tunable laser-triggered rupture and release of active enzymes into a hydrogel ambient. The advantages of this 3D printing-based method include (1) highly monodisperse capsules, (2) efficient encapsulation of biomolecular payloads, (3) precise spatial patterning of capsule arrays, (4) “on the fly” programmable reconfiguration of gradients, and (5) versatility for incorporation in hierarchical architectures. Indeed, 3D printing of programmable release capsules may represent a powerful new tool to enable spatiotemporal control over biomolecular gradients. PMID:26042472

  14. Effect of cold plasma pre-treatment on photocatalytic activity of 3D fabric loaded with nano-photocatalysts: Response surface methodology

    NASA Astrophysics Data System (ADS)

    Ghoreishian, Seyed Majid; Badii, Khashayar; Norouzi, Mohammad; Malek, Kaveh

    2016-03-01

    In this study, the physico-chemical effects occasioned by the cold plasma discharge (CPD) on the photo-decolorization of Reactive Orange 16 (RO16) by 3D fabrics (spacer fabrics) loaded with ZnO:TiO2 nano-photocatalysts (nphs) were optimized via response surface methodology (RSM). CPD was employed to improve the surface characteristics of the spacer fabrics for nphs loading. Surface morphology and color variation were studied utilizing scanning electron microscopy (SEM) and CIE-Lab system, respectively. The effect of CPD on the wetting ability of the spacer fabrics was examined using dynamic adsorption measurement (DAM). Also, X-ray fluorescence (XRF) was utilized to investigate the durability of the nphs on the spacer fabrics. All the experiments were implemented in a Box-Behnken design (BBD) with three independent variables (CPD treatment time, dye concentration and irradiation time) in order to optimize the decolorization of RO16. The anticipated values of the decolorization efficiency were found to be in excellent agreement with the experimental values (R2 = 0.9996, Adjusted R2 = 0.9992). The kinetic analysis demonstrated that the photocatalytic decolorization followed the Langmuir-Hinshelwood kinetic model. In conclusion, this heterogeneous photocatalytic process is capable of decolorizing and mineralizing azoic reactive dye in textile wastewater. Moreover, the results confirmed that RSM based on the BBD was a suitable method to optimize the operating conditions of RO16 degradation.

  15. An efficient and robust 3D mesh compression based on 3D watermarking and wavelet transform

    NASA Astrophysics Data System (ADS)

    Zagrouba, Ezzeddine; Ben Jabra, Saoussen; Didi, Yosra

    2011-06-01

    The compression and watermarking of 3D meshes are very important in many areas of activity including digital cinematography, virtual reality as well as CAD design. However, most studies on 3D watermarking and 3D compression are done independently. To verify a good trade-off between protection and a fast transfer of 3D meshes, this paper proposes a new approach which combines 3D mesh compression with mesh watermarking. This combination is based on a wavelet transformation. In fact, the used compression method is decomposed to two stages: geometric encoding and topologic encoding. The proposed approach consists to insert a signature between these two stages. First, the wavelet transformation is applied to the original mesh to obtain two components: wavelets coefficients and a coarse mesh. Then, the geometric encoding is done on these two components. The obtained coarse mesh will be marked using a robust mesh watermarking scheme. This insertion into coarse mesh allows obtaining high robustness to several attacks. Finally, the topologic encoding is applied to the marked coarse mesh to obtain the compressed mesh. The combination of compression and watermarking permits to detect the presence of signature after a compression of the marked mesh. In plus, it allows transferring protected 3D meshes with the minimum size. The experiments and evaluations show that the proposed approach presents efficient results in terms of compression gain, invisibility and robustness of the signature against of many attacks.

  16. 3D Virtual Reality for Teaching Astronomy

    NASA Astrophysics Data System (ADS)

    Speck, Angela; Ruzhitskaya, L.; Laffey, J.; Ding, N.

    2012-01-01

    We are developing 3D virtual learning environments (VLEs) as learning materials for an undergraduate astronomy course, in which will utilize advances both in technologies available and in our understanding of the social nature of learning. These learning materials will be used to test whether such VLEs can indeed augment science learning so that it is more engaging, active, visual and effective. Our project focuses on the challenges and requirements of introductory college astronomy classes. Here we present our virtual world of the Jupiter system and how we plan to implement it to allow students to learn course material - physical laws and concepts in astronomy - while engaging them into exploration of the Jupiter's system, encouraging their imagination, curiosity, and motivation. The VLE can allow students to work individually or collaboratively. The 3D world also provides an opportunity for research in astronomy education to investigate impact of social interaction, gaming features, and use of manipulatives offered by a learning tool on students’ motivation and learning outcomes. Use of this VLE is also a valuable source for exploration of how the learners’ spatial awareness can be enhanced by working in 3D environment. We will present the Jupiter-system environment along with a preliminary study of the efficacy and usability of our Jupiter 3D VLE.

  17. The mechanics and behavior of cliff swallows during tandem flights.

    PubMed

    Shelton, Ryan M; Jackson, Brandon E; Hedrick, Tyson L

    2014-08-01

    Cliff swallows (Petrochelidon pyrrhonota) are highly maneuverable social birds that often forage and fly in large open spaces. Here we used multi-camera videography to measure the three-dimensional kinematics of their natural flight maneuvers in the field. Specifically, we collected data on tandem flights, defined as two birds maneuvering together. These data permit us to evaluate several hypotheses on the high-speed maneuvering flight performance of birds. We found that high-speed turns are roll-based, but that the magnitude of the centripetal force created in typical maneuvers varied only slightly with flight speed, typically reaching a peak of ~2 body weights. Turning maneuvers typically involved active flapping rather than gliding. In tandem flights the following bird copied the flight path and wingbeat frequency (~12.3 Hz) of the lead bird while maintaining position slightly above the leader. The lead bird turned in a direction away from the lateral position of the following bird 65% of the time on average. Tandem flights vary widely in instantaneous speed (1.0 to 15.6 m s(-1)) and duration (0.72 to 4.71 s), and no single tracking strategy appeared to explain the course taken by the following bird. PMID:24855672

  18. Three-component synthesis of pyrano[2,3-d]-pyrimidine dione derivatives facilitated by sulfonic acid nanoporous silica (SBA-Pr-SO3H) and their docking and urease inhibitory activity

    PubMed Central

    2013-01-01

    Background A straightforward and efficient method for the synthesis of pyrano[2,3-d]pyrimidine diones derivatives from the reaction of barbituric acid, malononitrile and various aromatic aldehydes using SBA-Pr-SO3H as a nanocatalyst is reported. Results Reactions proceed with high efficiency under solvent free conditions. Urease inhibitory activity of pyrano[2,3-d]pyrimidine diones derivatives were tested against Jack bean urease using phenol red method. Three compounds of 4a, 4d and 4l were not active in urease inhibition test, but compound 4a displayed slight urease activation properties. Compounds 4b, 4k, 4f, 4e, 4j, 4g and 4c with hydrophobic substitutes on phenyl ring, showed good inhibitory activity (19.45-279.14 μM). Discussion The compounds with electron donating group and higher hydrophobic interaction with active site of enzyme prevents hydrolysis of substrate. Electron withdrawing groups such as nitro at different position and meta-methoxy reduced urease inhibitory activity. Substitution of both hydrogen of barbituric acid with methyl group will convert inhibitor to activator. PMID:23351402

  19. Synthesis and anti-DNA viral activities in vitro of certain 2,4-disubstituted-7-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)pyrrolo[2,3-d d pyrimidine nucleosides.

    PubMed

    Bhattacharya, B K; Ojwang, J O; Rando, R F; Huffman, J H; Revankar, G R

    1995-09-29

    Several novel 2,4-disubstituted-7-(2-deoxy-2-fluoro-beta-D- arabinofuranosyl)pyrrolo[2,3-d]pyrimidines have been synthesized and evaluated for their anti-human cytomegalovirus (HCMV), anti-hepatitis B virus (HBV), and anti-herpes simplex virus (HSV) activities in vitro. These nucleosides were prepared starting from 2-amino-4-chloro-7-(2-deoxy-2-fluoro- 3,5-di-O-benzoyl-beta-D-arabinofuranosyl)pyrrolo[2,3-d]pyrimidine (3), which in turn was synthesized by direct glycosylation of the sodium salt of 2-amino-4-chloropyrrolo[2,3-d]pyrimidine (1) with 2-deoxy-2-fluoro-3,5-di-O-benzoyl-alpha-D-arabinofuranosyl bromide (2). Displacement of the 4-chloro group of 3 with OH, NH2, NHOH, SH, and SeH nucleophiles furnished the corresponding nucleosides 6-8, 12, and 14, respectively. The 3'-deoxygenation of 2-amino-4-chloro-7- (2-deoxy-2-fluoro-beta-D-arabinofuranosyl)pyrrolo[2,3-d]pyrimidine (4) and subsequent amination gave 2,4-diamino-2',3'-dideoxy derivative 19. Catalytic hydrogenation of 3 followed by debenzoylation afforded 2-aminopyrrolo[2,3-d]pyrimidine nucleoside 23. Among the compounds evaluated for their ability to inhibit the growth of HCMV (strain AD169) in MRC-5 cells using a plaque reduction assay, only 7 was significantly active in vitro with a 50% inhibitory concentration (IC50) of 3.7 micrograms/mL (TI > 125), whereas the IC50 value of ganciclovir (DHPG) was 3.2 micrograms/mL. Strain D16 of HCMV was more resistant to 7 (IC50 11 micrograms/mL) than the AD169 strain. When 7 was tested in combination with DHPG, the resultant anti-HCMV activity was found to be moderately synergistic with no evidence of antagonism. Nucleoside 7 also reduced episomal HBV replication in human hepatoblastoma 2.2.15 cells with an IC50 of 0.7 micrograms/mL (TI > 143). Development of cells harboring HBV which had become resistant to the drug was not observed with 7. Compound 7 also exhibited significant activity against herpes simplex virus types 1 and 2 (IC50 of 4.1 and 6.3 micrograms

  20. 3D-QSAR and molecular mechanics study for the differences in the azole activity against yeastlike and filamentous fungi and their relation to P450DM inhibition. 1. 3-substituted-4(3H)-quinazolinones.

    PubMed

    Fratev, Filip; Benfenati, Emilio

    2005-01-01

    A combination between 3D-QSAR and molecular mechanics (MM)-docking study was used as a tool to detail and model the mechanism of action of 46 antifungal azoles. Two methods of alignment of the ligands were performed: (i) alignment of the main skeleton without substituents and (ii) alignment of a defined substructure. The best model is characterized by q(2) with the values of 0.70 for yeastlike (yeast), 0.66 for filamentous fungi, and 0.70 for the selectivity against filamentous fungi. 3D-QSAR regression maps derived from six models were used to identify the regions responsible for the differences in the compounds activity against yeast and filamentous fungi. The binding energy of the important substructures (Local Binding Energy-LBE) and its standard deviation were calculated in order to demonstrate quantitatively the contribution of substituents reflecting the diversity of the antifungal activity. The comparisons of these results with the same regions of the contour maps indicated a good correspondence between the 3D-QSAR and MM (LBE) approaches allowing association between the maps and the participating residues in the active sites of P450DM of C. albicans and A. fumigatus. The pi-pi interactions of two or more aromatic groups of the ligands with Phe228 and Tyr132 prove to be most important for the differences in activity against C. albicans. In A. fumigatus there was a better occupation of the inner central I-spiral in the areas around the heme. For the activity against A. fumigatus the pi-pi interactions of aromatic groups of the compounds with Phe509, Phe228, and Tyr132 are significant for the activity. PMID:15921453

  1. Optoplasmonics: hybridization in 3D

    NASA Astrophysics Data System (ADS)

    Rosa, L.; Gervinskas, G.; Žukauskas, A.; Malinauskas, M.; Brasselet, E.; Juodkazis, S.

    2013-12-01

    Femtosecond laser fabrication has been used to make hybrid refractive and di ractive micro-optical elements in photo-polymer SZ2080. For applications in micro- uidics, axicon lenses were fabricated (both single and arrays), for generation of light intensity patterns extending through the entire depth of a typically tens-of-micrometers deep channel. Further hybridisation of an axicon with a plasmonic slot is fabricated and demonstrated nu- merically. Spiralling chiral grooves were inscribed into a 100-nm-thick gold coating sputtered over polymerized micro-axicon lenses, using a focused ion beam. This demonstrates possibility of hybridisation between optical and plasmonic 3D micro-optical elements. Numerical modelling of optical performance by 3D-FDTD method is presented.

  2. 3-D Relativistic MHD Simulations

    NASA Astrophysics Data System (ADS)

    Nishikawa, K.-I.; Frank, J.; Koide, S.; Sakai, J.-I.; Christodoulou, D. M.; Sol, H.; Mutel, R. L.

    1998-12-01

    We present 3-D numerical simulations of moderately hot, supersonic jets propagating initially along or obliquely to the field lines of a denser magnetized background medium with Lorentz factors of W = 4.56 and evolving in a four-dimensional spacetime. The new results are understood as follows: Relativistic simulations have consistently shown that these jets are effectively heavy and so they do not suffer substantial momentum losses and are not decelerated as efficiently as their nonrelativistic counterparts. In addition, the ambient magnetic field, however strong, can be pushed aside with relative ease by the beam, provided that the degrees of freedom associated with all three spatial dimensions are followed self-consistently in the simulations. This effect is analogous to pushing Japanese ``noren'' or vertical Venetian blinds out of the way while the slats are allowed to bend in 3-D space rather than as a 2-D slab structure.

  3. Forensic 3D Scene Reconstruction

    SciTech Connect

    LITTLE,CHARLES Q.; PETERS,RALPH R.; RIGDON,J. BRIAN; SMALL,DANIEL E.

    1999-10-12

    Traditionally law enforcement agencies have relied on basic measurement and imaging tools, such as tape measures and cameras, in recording a crime scene. A disadvantage of these methods is that they are slow and cumbersome. The development of a portable system that can rapidly record a crime scene with current camera imaging, 3D geometric surface maps, and contribute quantitative measurements such as accurate relative positioning of crime scene objects, would be an asset to law enforcement agents in collecting and recording significant forensic data. The purpose of this project is to develop a feasible prototype of a fast, accurate, 3D measurement and imaging system that would support law enforcement agents to quickly document and accurately record a crime scene.

  4. Forensic 3D scene reconstruction

    NASA Astrophysics Data System (ADS)

    Little, Charles Q.; Small, Daniel E.; Peters, Ralph R.; Rigdon, J. B.

    2000-05-01

    Traditionally law enforcement agencies have relied on basic measurement and imaging tools, such as tape measures and cameras, in recording a crime scene. A disadvantage of these methods is that they are slow and cumbersome. The development of a portable system that can rapidly record a crime scene with current camera imaging, 3D geometric surface maps, and contribute quantitative measurements such as accurate relative positioning of crime scene objects, would be an asset to law enforcement agents in collecting and recording significant forensic data. The purpose of this project is to develop a fieldable prototype of a fast, accurate, 3D measurement and imaging system that would support law enforcement agents to quickly document and accurately record a crime scene.

  5. 360-degree 3D profilometry

    NASA Astrophysics Data System (ADS)

    Song, Yuanhe; Zhao, Hong; Chen, Wenyi; Tan, Yushan

    1997-12-01

    A new method of 360 degree turning 3D shape measurement in which light sectioning and phase shifting techniques are both used is presented in this paper. A sine light field is applied in the projected light stripe, meanwhile phase shifting technique is used to calculate phases of the light slit. Thereafter wrapped phase distribution of the slit is formed and the unwrapping process is made by means of the height information based on the light sectioning method. Therefore phase measuring results with better precision can be obtained. At last the target 3D shape data can be produced according to geometric relationships between phases and the object heights. The principles of this method are discussed in detail and experimental results are shown in this paper.

  6. 3D Printable Graphene Composite.

    PubMed

    Wei, Xiaojun; Li, Dong; Jiang, Wei; Gu, Zheming; Wang, Xiaojuan; Zhang, Zengxing; Sun, Zhengzong

    2015-01-01

    In human being's history, both the Iron Age and Silicon Age thrived after a matured massive processing technology was developed. Graphene is the most recent superior material which could potentially initialize another new material Age. However, while being exploited to its full extent, conventional processing methods fail to provide a link to today's personalization tide. New technology should be ushered in. Three-dimensional (3D) printing fills the missing linkage between graphene materials and the digital mainstream. Their alliance could generate additional stream to push the graphene revolution into a new phase. Here we demonstrate for the first time, a graphene composite, with a graphene loading up to 5.6 wt%, can be 3D printable into computer-designed models. The composite's linear thermal coefficient is below 75 ppm·°C(-1) from room temperature to its glass transition temperature (Tg), which is crucial to build minute thermal stress during the printing process. PMID:26153673

  7. 3D Printed Robotic Hand

    NASA Technical Reports Server (NTRS)

    Pizarro, Yaritzmar Rosario; Schuler, Jason M.; Lippitt, Thomas C.

    2013-01-01

    Dexterous robotic hands are changing the way robots and humans interact and use common tools. Unfortunately, the complexity of the joints and actuations drive up the manufacturing cost. Some cutting edge and commercially available rapid prototyping machines now have the ability to print multiple materials and even combine these materials in the same job. A 3D model of a robotic hand was designed using Creo Parametric 2.0. Combining "hard" and "soft" materials, the model was printed on the Object Connex350 3D printer with the purpose of resembling as much as possible the human appearance and mobility of a real hand while needing no assembly. After printing the prototype, strings where installed as actuators to test mobility. Based on printing materials, the manufacturing cost of the hand was $167, significantly lower than other robotic hands without the actuators since they have more complex assembly processes.

  8. 3D light scanning macrography.

    PubMed

    Huber, D; Keller, M; Robert, D

    2001-08-01

    The technique of 3D light scanning macrography permits the non-invasive surface scanning of small specimens at magnifications up to 200x. Obviating both the problem of limited depth of field inherent to conventional close-up macrophotography and the metallic coating required by scanning electron microscopy, 3D light scanning macrography provides three-dimensional digital images of intact specimens without the loss of colour, texture and transparency information. This newly developed technique offers a versatile, portable and cost-efficient method for the non-invasive digital and photographic documentation of small objects. Computer controlled device operation and digital image acquisition facilitate fast and accurate quantitative morphometric investigations, and the technique offers a broad field of research and educational applications in biological, medical and materials sciences. PMID:11489078

  9. 3D-graphite structure

    SciTech Connect

    Belenkov, E. A. Ali-Pasha, V. A.

    2011-01-15

    The structure of clusters of some new carbon 3D-graphite phases have been calculated using the molecular-mechanics methods. It is established that 3D-graphite polytypes {alpha}{sub 1,1}, {alpha}{sub 1,3}, {alpha}{sub 1,5}, {alpha}{sub 2,1}, {alpha}{sub 2,3}, {alpha}{sub 3,1}, {beta}{sub 1,2}, {beta}{sub 1,4}, {beta}{sub 1,6}, {beta}{sub 2,1}, and {beta}{sub 3,2} consist of sp{sup 2}-hybridized atoms, have hexagonal unit cells, and differ in regards to the structure of layers and order of their alternation. A possible way to experimentally synthesize new carbon phases is proposed: the polymerization and carbonization of hydrocarbon molecules.

  10. [Real time 3D echocardiography].

    PubMed

    Bauer, F; Shiota, T; Thomas, J D

    2001-07-01

    Three-dimensional representation of the heart is an old concern. Usually, 3D reconstruction of the cardiac mass is made by successive acquisition of 2D sections, the spatial localisation and orientation of which require complex guiding systems. More recently, the concept of volumetric acquisition has been introduced. A matricial emitter-receiver probe complex with parallel data processing provides instantaneous of a pyramidal 64 degrees x 64 degrees volume. The image is restituted in real time and is composed of 3 planes (planes B and C) which can be displaced in all spatial directions at any time during acquisition. The flexibility of this system of acquisition allows volume and mass measurement with greater accuracy and reproducibility, limiting inter-observer variability. Free navigation of the planes of investigation allows reconstruction for qualitative and quantitative analysis of valvular heart disease and other pathologies. Although real time 3D echocardiography is ready for clinical usage, some improvements are still necessary to improve its conviviality. Then real time 3D echocardiography could be the essential tool for understanding, diagnosis and management of patients. PMID:11494630

  11. [Real time 3D echocardiography

    NASA Technical Reports Server (NTRS)

    Bauer, F.; Shiota, T.; Thomas, J. D.

    2001-01-01

    Three-dimensional representation of the heart is an old concern. Usually, 3D reconstruction of the cardiac mass is made by successive acquisition of 2D sections, the spatial localisation and orientation of which require complex guiding systems. More recently, the concept of volumetric acquisition has been introduced. A matricial emitter-receiver probe complex with parallel data processing provides instantaneous of a pyramidal 64 degrees x 64 degrees volume. The image is restituted in real time and is composed of 3 planes (planes B and C) which can be displaced in all spatial directions at any time during acquisition. The flexibility of this system of acquisition allows volume and mass measurement with greater accuracy and reproducibility, limiting inter-observer variability. Free navigation of the planes of investigation allows reconstruction for qualitative and quantitative analysis of valvular heart disease and other pathologies. Although real time 3D echocardiography is ready for clinical usage, some improvements are still necessary to improve its conviviality. Then real time 3D echocardiography could be the essential tool for understanding, diagnosis and management of patients.

  12. Volume rendering for interactive 3D segmentation

    NASA Astrophysics Data System (ADS)

    Toennies, Klaus D.; Derz, Claus

    1997-05-01

    Combined emission/absorption and reflection/transmission volume rendering is able to display poorly segmented structures from 3D medical image sequences. Visual cues such as shading and color let the user distinguish structures in the 3D display that are incompletely extracted by threshold segmentation. In order to be truly helpful, analyzed information needs to be quantified and transferred back into the data. We extend our previously presented scheme for such display be establishing a communication between visual analysis and the display process. The main tool is a selective 3D picking device. For being useful on a rather rough segmentation, the device itself and the display offer facilities for object selection. Selective intersection planes let the user discard information prior to choosing a tissue of interest. Subsequently, a picking is carried out on the 2D display by casting a ray into the volume. The picking device is made pre-selective using already existing segmentation information. Thus, objects can be picked that are visible behind semi-transparent surfaces of other structures. Information generated by a later connected- component analysis can then be integrated into the data. Data examination is continued on an improved display letting the user actively participate in the analysis process. Results of this display-and-interaction scheme proved to be very effective. The viewer's ability to extract relevant information form a complex scene is combined with the computer's ability to quantify this information. The approach introduces 3D computer graphics methods into user- guided image analysis creating an analysis-synthesis cycle for interactive 3D segmentation.

  13. Long term dose monitoring onboard the European Columbus module of the international space station (ISS) in the frame of DOSIS and DOSIS 3D project - results from the active instruments

    NASA Astrophysics Data System (ADS)

    Burmeister, Soenke; Berger, Thomas; Reitz, Guenther; Boehme, Matthias; Haumann, Lutz; Labrenz, Johannes

    Besides the effects of the microgravity environment, and the psychological and psychosocial problems encountered in confined spaces, radiation is the main health detriment for long duration human space missions. The radiation environment encountered in space differs in nature from that on earth, consisting mostly of high energetic ions from protons up to iron, resulting in radiation levels far exceeding the ones encountered on earth for occupational radiation workers. Accurate knowledge of the physical characteristics of the space radiation field in dependence on the solar activity, the orbital parameters and the different shielding configurations of the International Space Station ISS is therefore needed. For the investigation of the spatial and temporal distribution of the radiation field inside the European COLUMBUS module the experiment DOSIS (Dose Distribution Inside the ISS) under the lead of DLR has been launched on July 15 (th) 2009 with STS-127 to the ISS. The experimental package was transferred from the Space Shuttle into COLUMBUS on July 18 (th) . It consists of a combination of passive detector packages (PDP) distributed at 11 locations inside the European Columbus Laboratory and two active radiation detectors (Dosimetry Telescopes = DOSTELs) with a DDPU (DOSTEL Data and Power Unit) in a Nomex pouch (DOSIS MAIN BOX) mounted at a fixed location beneath the European Physiology Module rack (EPM) inside COLUMBUS. The active components of the DOSIS experiment were operational from July 18 (th) 2009 to June 16 (th) 2011. After refurbishment the hardware has been reactivated on May 15 (th) 2012 as active part of the DOSIS 3D experiment and provides continuous data since this activation. The presentation will focus on the latest results from the two DOSTEL instruments as absorbed dose, dose equivalent and the related LET spectra gathered within the DOSIS (2009 - 2011) and DOSIS 3D (2012 - 2014) experiment. The CAU contributions to DOSIS and DOSIS 3D are

  14. GPU-Accelerated Denoising in 3D (GD3D)

    2013-10-01

    The raw computational power GPU Accelerators enables fast denoising of 3D MR images using bilateral filtering, anisotropic diffusion, and non-local means. This software addresses two facets of this promising application: what tuning is necessary to achieve optimal performance on a modern GPU? And what parameters yield the best denoising results in practice? To answer the first question, the software performs an autotuning step to empirically determine optimal memory blocking on the GPU. To answer themore » second, it performs a sweep of algorithm parameters to determine the combination that best reduces the mean squared error relative to a noiseless reference image.« less

  15. Magmatic Systems in 3-D

    NASA Astrophysics Data System (ADS)

    Kent, G. M.; Harding, A. J.; Babcock, J. M.; Orcutt, J. A.; Bazin, S.; Singh, S.; Detrick, R. S.; Canales, J. P.; Carbotte, S. M.; Diebold, J.

    2002-12-01

    Multichannel seismic (MCS) images of crustal magma chambers are ideal targets for advanced visualization techniques. In the mid-ocean ridge environment, reflections originating at the melt-lens are well separated from other reflection boundaries, such as the seafloor, layer 2A and Moho, which enables the effective use of transparency filters. 3-D visualization of seismic reflectivity falls into two broad categories: volume and surface rendering. Volumetric-based visualization is an extremely powerful approach for the rapid exploration of very dense 3-D datasets. These 3-D datasets are divided into volume elements or voxels, which are individually color coded depending on the assigned datum value; the user can define an opacity filter to reject plotting certain voxels. This transparency allows the user to peer into the data volume, enabling an easy identification of patterns or relationships that might have geologic merit. Multiple image volumes can be co-registered to look at correlations between two different data types (e.g., amplitude variation with offsets studies), in a manner analogous to draping attributes onto a surface. In contrast, surface visualization of seismic reflectivity usually involves producing "fence" diagrams of 2-D seismic profiles that are complemented with seafloor topography, along with point class data, draped lines and vectors (e.g. fault scarps, earthquake locations and plate-motions). The overlying seafloor can be made partially transparent or see-through, enabling 3-D correlations between seafloor structure and seismic reflectivity. Exploration of 3-D datasets requires additional thought when constructing and manipulating these complex objects. As numbers of visual objects grow in a particular scene, there is a tendency to mask overlapping objects; this clutter can be managed through the effective use of total or partial transparency (i.e., alpha-channel). In this way, the co-variation between different datasets can be investigated

  16. OBSIDIAN CLIFF OVERLOOKS THE EAST SIDE OF THE GRAND LOOP ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    OBSIDIAN CLIFF OVERLOOKS THE EAST SIDE OF THE GRAND LOOP ROAD. THE OBSIDIAN, A BLACK VOLCANIC GLASS, FORMED WHEN A LAVA FLOW CONTACTED GLACIAL ICE. IN THE CONSTRUCTION OF THE ROAD BY THE U.S. ARMY CORPS OF ENGINEERS, WORKERS CREATED THE LEDGE FOR THE ROAD BY BUILDING LARGE BONFIRES AGAINST THE CLIFF, THEN DASHING THE HEATED ROCK WITH COLD WATER, CAUSING IT TO SHATTER. - Grand Loop Road, Forming circuit between Mammoth Hot Springs, Norris Junction, Madison Junction, Old Faithful, Mammoth, Park County, WY

  17. Rates and Patterns of Postglacial Cliff Erosion in Yosemite Valley

    NASA Astrophysics Data System (ADS)

    Stock, G. M.

    2014-12-01

    The formerly glaciated granite cliffs of Yosemite Valley are now primarily modified by rockfalls, and vast accumulations of talus beneath the cliffs indicate the long-term importance of this process. Formation and weathering of exfoliation fractures provides a mechanism for relatively steady cliff erosion over thousand-year timescales, but how important are larger events? Yosemite Valley presents an ideal setting for investigating postglacial cliff erosion because (1) Last Glacial Maximum deglaciation left a wide, flat valley floor that has experienced only a few meters of subsequent aggradation, (2) deglaciation is known to have occurred ~15 ka, (3) lidar-based digital elevation models reveal postglacial talus accumulations in three dimensions, (4) talus deposits have been precisely dated by Be-10, and (5) historical rockfalls are well documented. Since deglaciation, ~180 million m3 of rock has been shed from 53 million m2 of cliffs, representing a postglacial cliff retreat rate of ~0.2 mm/yr - among the faster landscape erosion rates measured in the Sierra Nevada. The average annual talus accumulation rate over the past 15 kyr is about 12,000 m3/yr, but evidence suggests that accumulation rates were punctuated by infrequent, extremely large events. Ten extremely large rockfalls, dated by Be-10, occurred between 13.2 and 1.0 ka and were likely seismically triggered. Volumetrically, these events represent about one-fifth of the total postglacial talus volume. Historical talus accumulations are similarly punctuated. A database of historical rockfalls documents ~1.55 million m3 of rock that has fallen from the valley walls since 1857, yielding a historical cliff retreat rate of ~0.2 mm/yr and an average annual accumulation rate of about 10,000 m3/yr, similar to rates averaged over 15 kyr. However, here again talus accumulation is dominated by a few extremely large (≥200,000 m3) events; a single 600,000 m3 rockfall in 1987 accounts for nearly one-third of the total

  18. The Echo Cliffs Precariously Balanced Rock; Discovery and Terrestrial Laser Scanning

    NASA Astrophysics Data System (ADS)

    Hudnut, K. W.; Amidon, W. H.; Bawden, G. W.; Brune, J. N.; Bond, S.; Graves, R. W.; Haddad, D. E.; Limaye, A.; Lynch, D. K.; Phillips, D. A.; Pounders, E.; Rood, D. H.

    2009-12-01

    We investigate a previously undocumented Precariously Balanced Rock (PBR) located above Echo Cliffs in the western Santa Monica Mountains, using Terrestrial Laser Scanning (TLS). We present the merged and aligned point cloud of TLS data (over 42 million points) and photos to document the Echo Cliffs PBR site. We also present our interpretations of the site, of its geomorphic development, and its possible significance for seismic hazards in the Los Angeles region. The rock lies above the ramp in the fault propagation fold structure that has been interpreted by Davis and Namson as an active structure that may pose a major seismic hazard to the Los Angeles area. The Echo Cliffs PBR stands at just over 14 meters in height, so assuming it acts as an inverse pendulum, it has a 3 to 4 second period of oscillation. This period corresponds to the oscillatory period of a 30 to 40 story building. The rock withstood ground motions during the 1994 Northridge earthquake that we estimate to have been 0.2 g (PGA) and 12 cm/sec (PGV) at this site. It is expected that the Echo Cliffs PBR may provide important constraints on scenario ground motions and thereby improve future simulations. We believe that this is the first application of TLS methods on PBR's, and we find that the high resolution provided by TLS allows us to characterize the detailed shape of the PBR itself, as well as key details of the interface between the rock and pedestal at the cm-level. In addition, the cliff band near the PBR and the geomorphic context of the surrounding hill slope area was also scanned at a coarser resolution, providing data that help to understand the processes by which the PBR formed. An advantage of TLS is that it provides an image of the outline of the rock-pedestal contact surface and adjacent non-contacting surfaces. The TLS also helps quantify the effects of shielding on cosmogenic nuclide production, enhancing the accuracy of our surface exposure age dating.

  19. Design, synthesis, biological activities, and 3D-QSAR of new N,N'-diacylhydrazines containing 2-(2,4-dichlorophenoxy)propane moiety.

    PubMed

    Liu, Xing-Hai; Pan, Li; Ma, Yi; Weng, Jian-Quan; Tan, Cheng-Xia; Li, Yong-Hong; Shi, Yan-Xia; Li, Bao-Ju; Li, Zheng-Ming; Zhang, Yong-Gang

    2011-10-01

    A series of new N,N'-diacylhydrazine derivatives were synthesized efficiently under microwave irradiation. Their structures were characterized by (1) H NMR, MS, and elemental analysis. Various biological activities of these compounds were tested. Most of them exhibited higher herbicidal activities against dicotyledonous weeds than monocotyledonous weeds. In addition, favorable in vivo fungicidal activities were also found of these compounds against Cladosporium cucumerinum, Corynespora cassiicola, Sclerotinia sclerotiorum(Lib.)de Bary, Erysiphe cichoracearum, and Colletotrichum orbiculare (Berk aLMont) Arx. All compounds displayed excellent plant growth regulatory activities: 100% inhibition was achieved against the radicle growth of cucumber. To further investigate the structure-activity relationship, comparative molecular field analysis was performed on the basis of herbicidal activity data, resulting in a statistically reliable model with good predictive power (r(2) = 0.913, q(2) =0.556). Based on the calculation, five additional novel compounds were designed and synthesized. Satisfyingly, compound 4u displayed excellent herbicidal activity (94.7%) at 1500 g/ha, although it is less active than 2,4-D. Meanwhile, this compound also exhibited good fungicidal activity against C. orbiculare (Berk aLMont) Arx (82.16%). PMID:21816005

  20. Visualization of 3D Geological Models on Google Earth

    NASA Astrophysics Data System (ADS)

    Choi, Y.; Um, J.; Park, M.

    2013-05-01

    Google Earth combines satellite imagery, aerial photography, thematic maps and various data sets to make a three-dimensional (3D) interactive image of the world. Currently, Google Earth is a popular visualization tool in a variety of fields and plays an increasingly important role not only for private users in daily life, but also for scientists, practitioners, policymakers and stakeholders in research and application. In this study, a method to visualize 3D geological models on Google Earth is presented. COLLAborative Design Activity (COLLADA, an open standard XML schema for establishing interactive 3D applications) was used to represent different 3D geological models such as borehole, fence section, surface-based 3D volume and 3D grid by triangle meshes (a set of triangles connected by their common edges or corners). In addition, we designed Keyhole Markup Language (KML, the XML-based scripting language of Google Earth) codes to import the COLLADA files into the 3D render window of Google Earth. The method was applied to the Grosmont formation in Alberta, Canada. The application showed that the combination of COLLADA and KML enables Google Earth to effectively visualize 3D geological structures and properties.; Visualization of the (a) boreholes, (b) fence sections, (c) 3D volume model and (d) 3D grid model of Grossmont formation on Google Earth

  1. Characterization of callase (β-1,3-D-glucanase) activity during microsporogenesis in the sterile anthers of Allium sativum L. and the fertile anthers of A. atropurpureum.

    PubMed

    Winiarczyk, Krystyna; Jaroszuk-Ściseł, Jolanta; Kupisz, Kamila

    2012-06-01

    We examined callase activity in anthers of sterile Allium sativum (garlic) and fertile Allium atropurpureum. In A. sativum, a species that produces sterile pollen and propagates only vegetatively, callase was extracted from the thick walls of A. sativum microspore tetrads exhibited maximum activity at pH 4.8, and the corresponding in vivo values ranged from 4.5 to 5.0. Once microspores were released, in vitro callase activity peaked at three distinct pH values, reflecting the presence of three callase isoforms. One isoform, which was previously identified in the tetrad stage, displayed maximum activity at pH 4.8, and the remaining two isoforms, which were novel, were most active at pH 6.0 and 7.3. The corresponding in vivo values ranged from pH 4.75 to 6.0. In contrast, in A. atropurpureum, a sexually propagating species, three callase isoforms, active at pH 4.8-5.2, 6.1, and 7.3, were identified in samples of microsporangia that had released their microspores. The corresponding in vivo value for this plant was 5.9. The callose wall persists around A. sativum meiotic cells, whereas only one callase isoform, with an optimum activity of pH 4.8, is active in the acidic environment of the microsporangium. However, this isoform is degraded when the pH rises to 6.0 and two other callase isoforms, maximally active at pH 6.0 and 7.3, appear. Thus, factors that alter the pH of the microsporangium may indirectly affect the male gametophyte development by modulating the activity of callase and thereby regulating the degradation of the callose wall. PMID:22438078

  2. The potential of low-cost RPAS for multi-view reconstruction of rock cliffs

    NASA Astrophysics Data System (ADS)

    Ettore Guccione, Davide; Thoeni, Klaus; Santise, Marina; Giacomini, Anna; Roncella, Riccardo; Forlani, Gianfranco

    2016-04-01

    RPAS, also known as drones or UAVs, have been used in military applications for many years. Nevertheless, the technology has become accessible to everyone only in recent years (Westoby et al., 2012; Nex and Remondino, 2014). Electric multirotor helicopters or multicopters have become one of the most exciting developments and several off-the-shelf platforms (including camera) are now available. In particular, RPAS can provide 3D models of sub-vertical rock faces, which for instance are needed for rockfall hazard assessments along road cuts and very steep mountains. The current work investigates the potential of two low-cost off-the-shelf quadcopters equipped with digital cameras for multi-view reconstruction of sub-vertical rock cliffs. The two platforms used are a DJI Phantom 1 (P1) equipped with a Gopro Hero 3+ (12MP) and a DJI Phantom 3 Professional (P3). The latter comes with an integrated 12MP camera mounted on a 3-axis gimbal. Both platforms cost less than 1.500€ including camera. The study area is a small rock cliff near the Callaghan Campus of the University of Newcastle (Thoeni et al., 2014). The wall is partly smooth with some evident geological features such as non-persistent joints and sharp edges. Several flights were performed with both cameras set in time-lapse mode. Hence, images were taken automatically but the flights were performed manually since the investigated rock face is very irregular which required adjusting the yaw and roll for optimal coverage since the flights were performed very close to the cliff face. The digital images were processed with a commercial SfM software package. Thereby, several processing options and camera networks were investigated in order to define the most accurate configuration. Firstly, the difference between the use of coded ground control targets versus natural features was studied. Coded targets generally provide the best accuracy but they need to be placed on the surface which is not always possible as rock

  3. Techniques for interactive 3-D scientific visualization

    SciTech Connect

    Glinert, E.P. . Dept. of Computer Science); Blattner, M.M. Hospital and Tumor Inst., Houston, TX . Dept. of Biomathematics California Univ., Davis, CA . Dept. of Applied Science Lawrence Livermore National Lab., CA ); Becker, B.G. . Dept. of Applied Science Lawrence Livermore National La

    1990-09-24

    Interest in interactive 3-D graphics has exploded of late, fueled by (a) the allure of using scientific visualization to go where no-one has gone before'' and (b) by the development of new input devices which overcome some of the limitations imposed in the past by technology, yet which may be ill-suited to the kinds of interaction required by researchers active in scientific visualization. To resolve this tension, we propose a flat 5-D'' environment in which 2-D graphics are augmented by exploiting multiple human sensory modalities using cheap, conventional hardware readily available with personal computers and workstations. We discuss how interactions basic to 3-D scientific visualization, like searching a solution space and comparing two such spaces, are effectively carried out in our environment. Finally, we describe 3DMOVE, an experimental microworld we have implemented to test out some of our ideas. 40 refs., 4 figs.

  4. Design, Synthesis, Antifungal Activities and 3D-QSAR of New N,N′-Diacylhydrazines Containing 2,4-Dichlorophenoxy Moiety

    PubMed Central

    Sun, Na-Bo; Shi, Yan-Xia; Liu, Xing-Hai; Ma, Yi; Tan, Cheng-Xia; Weng, Jian-Quan; Jin, Jian-Zhong; Li, Bao-Ju

    2013-01-01

    A series of new N,N′-diacylhydrazine derivatives were designed and synthesized. Their structures were verified by 1H-NMR, mass spectra (MS) and elemental analysis. The antifungal activities of these N,N′-diacylhydrazines were evaluated. The bioassay results showed that most of these N,N′-diacylhydrazines showed excellent antifungal activities against Cladosporium cucumerinum, Corynespora cassiicola, Sclerotinia sclerotiorum, Erysiphe cichoracearum, and Colletotrichum orbiculare in vivo. The half maximal effective concentration (EC50) of one of the compounds was also determined, and found to be comparable with a commercial drug. To further investigate the structure–activity relationship, comparative molecular field analysis (CoMFA) was performed on the basis of antifungal activity data. Both the steric and electronic field distributions of CoMFA are in good agreement in this study. PMID:24189221

  5. Design, synthesis, antifungal activities and 3D-QSAR of new N,N'-diacylhydrazines containing 2,4-dichlorophenoxy moiety.

    PubMed

    Sun, Na-Bo; Shi, Yan-Xia; Liu, Xing-Hai; Ma, Yi; Tan, Cheng-Xia; Weng, Jian-Quan; Jin, Jian-Zhong; Li, Bao-Ju

    2013-01-01

    A series of new N,N'-diacylhydrazine derivatives were designed and synthesized. Their structures were verified by 1H-NMR, mass spectra (MS) and elemental analysis. The antifungal activities of these N,N'-diacylhydrazines were evaluated. The bioassay results showed that most of these N,N'-diacylhydrazines showed excellent antifungal activities against Cladosporium cucumerinum, Corynespora cassiicola, Sclerotinia sclerotiorum, Erysiphe cichoracearum, and Colletotrichum orbiculare in vivo. The half maximal effective concentration (EC50) of one of the compounds was also determined, and found to be comparable with a commercial drug. To further investigate the structure-activity relationship, comparative molecular field analysis (CoMFA) was performed on the basis of antifungal activity data. Both the steric and electronic field distributions of CoMFA are in good agreement in this study. PMID:24189221

  6. Coastal cliff geometry derived from structure-from-motion photogrammetry at Stara Ba\\vska, Krk Island, Croatia

    NASA Astrophysics Data System (ADS)

    Ružić, Igor; Marović, Ivan; Benac, Čedomir; Ilić, Suzana

    2014-12-01

    The aim of this study was to examine the capability of structure-from-motion photogrammetry in defining the geometry of cliffs and undercuts in rocks of complex geomorphology. A case site was chosen along pocket beaches near the village of Stara Ba\\vska on the Adriatic Sea island of Krk, Gulf of Kvarner, Croatia, where cliff erosion of 5 m in breccias was identified by comparison of aerial photographs from 1960 and 2004. The 3D point cloud was derived from approx. 800 photos taken on 9 January 2014 by a single camera from various elevations and angles, and processed using the online software ReCap (Autodesk). Data acquisition was found to be quick and the method easy to implement. The difference between the georeferenced 3D cloud points and an RTK-GPS survey was 7 cm, i.e. within the limits of RTK-GPS precision. Quantifying the spatial variation in undercut geometries revealed that the deepest and largest (17 m3) undercut was in the south-eastern sector of the beach. Reconstructing the detailed geomorphology of this 3.8-m-deep undercut convincingly demonstrates the high efficiency of the method. Such assessments of spatiotemporal changes in undercut and overhang volumes can prove useful for evaluations of cliff erosion risk. Coupled with the low cost and relatively simple application, this is evidently an attractive technique for meaningful geotechnical and coastal engineering monitoring in the future on the island of Krk and, for that matter, also on other Adriatic islands and in similar settings worldwide.

  7. 77 FR 34093 - License Renewal for Calvert Cliffs Nuclear Power Plant, LLC's

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-08

    ... COMMISSION License Renewal for Calvert Cliffs Nuclear Power Plant, LLC's AGENCY: Nuclear Regulatory... Cliffs Nuclear Power Plant site near Lusby, Maryland. The NRC has prepared an Environmental Assessment... dated September 17, 2010, Calvert Cliffs Nuclear Power Plant, LLC (CCNPP) submitted an application...

  8. A Clean Adirondack (3-D)

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This is a 3-D anaglyph showing a microscopic image taken of an area measuring 3 centimeters (1.2 inches) across on the rock called Adirondack. The image was taken at Gusev Crater on the 33rd day of the Mars Exploration Rover Spirit's journey (Feb. 5, 2004), after the rover used its rock abrasion tool brush to clean the surface of the rock. Dust, which was pushed off to the side during cleaning, can still be seen to the left and in low areas of the rock.

  9. Making Inexpensive 3-D Models

    NASA Astrophysics Data System (ADS)

    Manos, Harry

    2016-03-01

    Visual aids are important to student learning, and they help make the teacher's job easier. Keeping with the TPT theme of "The Art, Craft, and Science of Physics Teaching," the purpose of this article is to show how teachers, lacking equipment and funds, can construct a durable 3-D model reference frame and a model gravity well tailored to specific class lessons. Most of the supplies are readily available in the home or at school: rubbing alcohol, a rag, two colors of spray paint, art brushes, and masking tape. The cost of these supplies, if you don't have them, is less than 20.

  10. What Lies Ahead (3-D)

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This 3-D cylindrical-perspective mosaic taken by the navigation camera on the Mars Exploration Rover Spirit on sol 82 shows the view south of the large crater dubbed 'Bonneville.' The rover will travel toward the Columbia Hills, seen here at the upper left. The rock dubbed 'Mazatzal' and the hole the rover drilled in to it can be seen at the lower left. The rover's position is referred to as 'Site 22, Position 32.' This image was geometrically corrected to make the horizon appear flat.

  11. Vacant Lander in 3-D

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This 3-D image captured by the Mars Exploration Rover Opportunity's rear hazard-identification camera shows the now-empty lander that carried the rover 283 million miles to Meridiani Planum, Mars. Engineers received confirmation that Opportunity's six wheels successfully rolled off the lander and onto martian soil at 3:01 a.m. PST, January 31, 2004, on the seventh martian day, or sol, of the mission. The rover is approximately 1 meter (3 feet) in front of the lander, facing north.

  12. Growth Control of MoS2 Nanosheets on Carbon Cloth for Maximum Active Edges Exposed: An Excellent Hydrogen Evolution 3D Cathode.

    PubMed

    Zhang, Nan; Gan, Shiyu; Wu, Tongshun; Ma, Weiguang; Han, Dongxue; Niu, Li

    2015-06-10

    To greatly improve the hydrogen evolution reaction (HER) performance, it is the key approach to expose as many active edges of MoS2 as possible. This target is the research hotspot and difficulty of MoS2 which is a promising HER catalyst. In this work, we realized the active-edges control of MoS2 nanosheets on carbon cloth (CC) by growth control during the synthesis procedure. Moreover, MoS2 nanosheets vertically grown on carbon cloth (MoS2⊥CC) was confirmed to be the best morphology with maximum active edges exposed. Multifactors structure control resulted in abundant active-edges exposure and effective electron delivery, thus excellent HER activity. This three-dimensional cathode, MoS2⊥CC, can reach a great current density of 200 mA/cm(2) at a small overpotential of 205 mV. The preeminent HER performance can rival the best MoS2-based catalyst ever reported. PMID:25980786

  13. Positional Awareness Map 3D (PAM3D)

    NASA Technical Reports Server (NTRS)

    Hoffman, Monica; Allen, Earl L.; Yount, John W.; Norcross, April Louise

    2012-01-01

    The Western Aeronautical Test Range of the National Aeronautics and Space Administration s Dryden Flight Research Center needed to address the aging software and hardware of its current situational awareness display application, the Global Real-Time Interactive Map (GRIM). GRIM was initially developed in the late 1980s and executes on older PC architectures using a Linux operating system that is no longer supported. Additionally, the software is difficult to maintain due to its complexity and loss of developer knowledge. It was decided that a replacement application must be developed or acquired in the near future. The replacement must provide the functionality of the original system, the ability to monitor test flight vehicles in real-time, and add improvements such as high resolution imagery and true 3-dimensional capability. This paper will discuss the process of determining the best approach to replace GRIM, and the functionality and capabilities of the first release of the Positional Awareness Map 3D.

  14. 3D IC for Future HEP Detectors

    SciTech Connect

    Thom, J.; Lipton, R.; Heintz, U.; Johnson, M.; Narain, M.; Badman, R.; Spiegel, L.; Triphati, M.; Deptuch, G.; Kenney, C.; Parker, S.; Ye, Z.; Siddons, D.

    2014-11-07

    Three dimensional integrated circuit technologies offer the possibility of fabricating large area arrays of sensors integrated with complex electronics with minimal dead area, which makes them ideally suited for applications at the LHC upgraded detectors and other future detectors. Here we describe ongoing R&D efforts to demonstrate functionality of components of such detectors. This also includes the study of integrated 3D electronics with active edge sensors to produce "active tiles" which can be tested and assembled into arrays of arbitrary size with high yield.

  15. 3D IC for future HEP detectors

    NASA Astrophysics Data System (ADS)

    Thom, J.; Lipton, R.; Heintz, U.; Johnson, M.; Narain, M.; Badman, R.; Spiegel, L.; Triphati, M.; Deptuch, G.; Kenney, C.; Parker, S.; Ye, Z.; Siddons, D. P.

    2014-11-01

    Three dimensional integrated circuit technologies offer the possibility of fabricating large area arrays of sensors integrated with complex electronics with minimal dead area, which makes them ideally suited for applications at the LHC upgraded detectors and other future detectors. We describe ongoing R&D efforts to demonstrate functionality of components of such detectors. This includes the study of integrated 3D electronics with active edge sensors to produce "active tiles" which can be tested and assembled into arrays of arbitrary size with high yield.

  16. Educational Visualizations in 3D Collaborative Virtual Environments: A Methodology

    ERIC Educational Resources Information Center

    Fominykh, Mikhail; Prasolova-Forland, Ekaterina

    2012-01-01

    Purpose: Collaborative virtual environments (CVEs) have become increasingly popular in educational settings and the role of 3D content is becoming more and more important. Still, there are many challenges in this area, such as lack of empirical studies that provide design for educational activities in 3D CVEs and lack of norms of how to support…

  17. 3D Printable Graphene Composite

    NASA Astrophysics Data System (ADS)

    Wei, Xiaojun; Li, Dong; Jiang, Wei; Gu, Zheming; Wang, Xiaojuan; Zhang, Zengxing; Sun, Zhengzong

    2015-07-01

    In human being’s history, both the Iron Age and Silicon Age thrived after a matured massive processing technology was developed. Graphene is the most recent superior material which could potentially initialize another new material Age. However, while being exploited to its full extent, conventional processing methods fail to provide a link to today’s personalization tide. New technology should be ushered in. Three-dimensional (3D) printing fills the missing linkage between graphene materials and the digital mainstream. Their alliance could generate additional stream to push the graphene revolution into a new phase. Here we demonstrate for the first time, a graphene composite, with a graphene loading up to 5.6 wt%, can be 3D printable into computer-designed models. The composite’s linear thermal coefficient is below 75 ppm·°C-1 from room temperature to its glass transition temperature (Tg), which is crucial to build minute thermal stress during the printing process.

  18. 3D acoustic atmospheric tomography

    NASA Astrophysics Data System (ADS)

    Rogers, Kevin; Finn, Anthony

    2014-10-01

    This paper presents a method for tomographically reconstructing spatially varying 3D atmospheric temperature profiles and wind velocity fields based. Measurements of the acoustic signature measured onboard a small Unmanned Aerial Vehicle (UAV) are compared to ground-based observations of the same signals. The frequency-shifted signal variations are then used to estimate the acoustic propagation delay between the UAV and the ground microphones, which are also affected by atmospheric temperature and wind speed vectors along each sound ray path. The wind and temperature profiles are modelled as the weighted sum of Radial Basis Functions (RBFs), which also allow local meteorological measurements made at the UAV and ground receivers to supplement any acoustic observations. Tomography is used to provide a full 3D reconstruction/visualisation of the observed atmosphere. The technique offers observational mobility under direct user control and the capacity to monitor hazardous atmospheric environments, otherwise not justifiable on the basis of cost or risk. This paper summarises the tomographic technique and reports on the results of simulations and initial field trials. The technique has practical applications for atmospheric research, sound propagation studies, boundary layer meteorology, air pollution measurements, analysis of wind shear, and wind farm surveys.

  19. 3D Printed Bionic Ears

    PubMed Central

    Mannoor, Manu S.; Jiang, Ziwen; James, Teena; Kong, Yong Lin; Malatesta, Karen A.; Soboyejo, Winston O.; Verma, Naveen; Gracias, David H.; McAlpine, Michael C.

    2013-01-01

    The ability to three-dimensionally interweave biological tissue with functional electronics could enable the creation of bionic organs possessing enhanced functionalities over their human counterparts. Conventional electronic devices are inherently two-dimensional, preventing seamless multidimensional integration with synthetic biology, as the processes and materials are very different. Here, we present a novel strategy for overcoming these difficulties via additive manufacturing of biological cells with structural and nanoparticle derived electronic elements. As a proof of concept, we generated a bionic ear via 3D printing of a cell-seeded hydrogel matrix in the precise anatomic geometry of a human ear, along with an intertwined conducting polymer consisting of infused silver nanoparticles. This allowed for in vitro culturing of cartilage tissue around an inductive coil antenna in the ear, which subsequently enables readout of inductively-coupled signals from cochlea-shaped electrodes. The printed ear exhibits enhanced auditory sensing for radio frequency reception, and complementary left and right ears can listen to stereo audio music. Overall, our approach suggests a means to intricately merge biologic and nanoelectronic functionalities via 3D printing. PMID:23635097

  20. 3-D Relativistic MHD Simulations

    NASA Astrophysics Data System (ADS)

    Nishikaw, K.-I.; Frank, J.; Christodoulou, D. M.; Koide, S.; Sakai, J.-I.; Sol, H.; Mutel, R. L.

    1998-12-01

    We present 3-D numerical simulations of moderately hot, supersonic jets propagating initially along or obliquely to the field lines of a denser magnetized background medium with Lorentz factors of W=4.56 and evolving in a four-dimensional spacetime. The new results are understood as follows: Relativistic simulations have consistently shown that these jets are effectively heavy and so they do not suffer substantial momentum losses and are not decelerated as efficiently as their nonrelativistic counterparts. In addition, the ambient magnetic field, however strong, can be pushed aside with relative ease by the beam, provided that the degrees of freedom associated with all three spatial dimensions are followed self-consistently in the simulations. This effect is analogous to pushing Japanese ``noren'' or vertical Venetian blinds out of the way while the slats are allowed to bend in 3-D space rather than as a 2-D slab structure. We also simulate jets with the more realistic initial conditions for injecting jets for helical mangetic field, perturbed density, velocity, and internal energy, which are supposed to be caused in the process of jet generation. Three possible explanations for the observed variability are (i) tidal disruption of a star falling into the black hole, (ii) instabilities in the relativistic accretion disk, and (iii) jet-related PRocesses. New results will be reported at the meeting.

  1. 3D printed bionic ears.

    PubMed

    Mannoor, Manu S; Jiang, Ziwen; James, Teena; Kong, Yong Lin; Malatesta, Karen A; Soboyejo, Winston O; Verma, Naveen; Gracias, David H; McAlpine, Michael C

    2013-06-12

    The ability to three-dimensionally interweave biological tissue with functional electronics could enable the creation of bionic organs possessing enhanced functionalities over their human counterparts. Conventional electronic devices are inherently two-dimensional, preventing seamless multidimensional integration with synthetic biology, as the processes and materials are very different. Here, we present a novel strategy for overcoming these difficulties via additive manufacturing of biological cells with structural and nanoparticle derived electronic elements. As a proof of concept, we generated a bionic ear via 3D printing of a cell-seeded hydrogel matrix in the anatomic geometry of a human ear, along with an intertwined conducting polymer consisting of infused silver nanoparticles. This allowed for in vitro culturing of cartilage tissue around an inductive coil antenna in the ear, which subsequently enables readout of inductively-coupled signals from cochlea-shaped electrodes. The printed ear exhibits enhanced auditory sensing for radio frequency reception, and complementary left and right ears can listen to stereo audio music. Overall, our approach suggests a means to intricately merge biologic and nanoelectronic functionalities via 3D printing. PMID:23635097

  2. 3D Printable Graphene Composite

    PubMed Central

    Wei, Xiaojun; Li, Dong; Jiang, Wei; Gu, Zheming; Wang, Xiaojuan; Zhang, Zengxing; Sun, Zhengzong

    2015-01-01

    In human being’s history, both the Iron Age and Silicon Age thrived after a matured massive processing technology was developed. Graphene is the most recent superior material which could potentially initialize another new material Age. However, while being exploited to its full extent, conventional processing methods fail to provide a link to today’s personalization tide. New technology should be ushered in. Three-dimensional (3D) printing fills the missing linkage between graphene materials and the digital mainstream. Their alliance could generate additional stream to push the graphene revolution into a new phase. Here we demonstrate for the first time, a graphene composite, with a graphene loading up to 5.6 wt%, can be 3D printable into computer-designed models. The composite’s linear thermal coefficient is below 75 ppm·°C−1 from room temperature to its glass transition temperature (Tg), which is crucial to build minute thermal stress during the printing process. PMID:26153673

  3. 3D medical thermography device

    NASA Astrophysics Data System (ADS)

    Moghadam, Peyman

    2015-05-01

    In this paper, a novel handheld 3D medical thermography system is introduced. The proposed system consists of a thermal-infrared camera, a color camera and a depth camera rigidly attached in close proximity and mounted on an ergonomic handle. As a practitioner holding the device smoothly moves it around the human body parts, the proposed system generates and builds up a precise 3D thermogram model by incorporating information from each new measurement in real-time. The data is acquired in motion, thus it provides multiple points of view. When processed, these multiple points of view are adaptively combined by taking into account the reliability of each individual measurement which can vary due to a variety of factors such as angle of incidence, distance between the device and the subject and environmental sensor data or other factors influencing a confidence of the thermal-infrared data when captured. Finally, several case studies are presented to support the usability and performance of the proposed system.

  4. 3D Ion Temperature Reconstruction

    NASA Astrophysics Data System (ADS)

    Tanabe, Hiroshi; You, Setthivoine; Balandin, Alexander; Inomoto, Michiaki; Ono, Yasushi

    2009-11-01

    The TS-4 experiment at the University of Tokyo collides two spheromaks to form a single high-beta compact toroid. Magnetic reconnection during the merging process heats and accelerates the plasma in toroidal and poloidal directions. The reconnection region has a complex 3D topology determined by the pitch of the spheromak magnetic fields at the merging plane. A pair of multichord passive spectroscopic diagnostics have been established to measure the ion temperature and velocity in the reconnection volume. One setup measures spectral lines across a poloidal plane, retrieving velocity and temperature from Abel inversion. The other, novel setup records spectral lines across another section of the plasma and reconstructs velocity and temperature from 3D vector and 2D scalar tomography techniques. The magnetic field linking both measurement planes is determined from in situ magnetic probe arrays. The ion temperature is then estimated within the volume between the two measurement planes and at the reconnection region. The measurement is followed over several repeatable discharges to follow the heating and acceleration process during the merging reconnection.

  5. LOTT RANCH 3D PROJECT

    SciTech Connect

    Larry Lawrence; Bruce Miller

    2004-09-01

    The Lott Ranch 3D seismic prospect located in Garza County, Texas is a project initiated in September of 1991 by the J.M. Huber Corp., a petroleum exploration and production company. By today's standards the 126 square mile project does not seem monumental, however at the time it was conceived it was the most intensive land 3D project ever attempted. Acquisition began in September of 1991 utilizing GEO-SEISMIC, INC., a seismic data contractor. The field parameters were selected by J.M. Huber, and were of a radical design. The recording instruments used were GeoCor IV amplifiers designed by Geosystems Inc., which record the data in signed bit format. It would not have been practical, if not impossible, to have processed the entire raw volume with the tools available at that time. The end result was a dataset that was thought to have little utility due to difficulties in processing the field data. In 1997, Yates Energy Corp. located in Roswell, New Mexico, formed a partnership to further develop the project. Through discussions and meetings with Pinnacle Seismic, it was determined that the original Lott Ranch 3D volume could be vastly improved upon reprocessing. Pinnacle Seismic had shown the viability of improving field-summed signed bit data on smaller 2D and 3D projects. Yates contracted Pinnacle Seismic Ltd. to perform the reprocessing. This project was initiated with high resolution being a priority. Much of the potential resolution was lost through the initial summing of the field data. Modern computers that are now being utilized have tremendous speed and storage capacities that were cost prohibitive when this data was initially processed. Software updates and capabilities offer a variety of quality control and statics resolution, which are pertinent to the Lott Ranch project. The reprocessing effort was very successful. The resulting processed data-set was then interpreted using modern PC-based interpretation and mapping software. Production data, log data

  6. Meet the 2008 Cliff Weiss Memorial Essay Contest Winners

    ERIC Educational Resources Information Center

    Techniques: Connecting Education and Careers (J3), 2008

    2008-01-01

    This article presents the winners of the 2008 Cliff Weiss Memorial Essay Contest and their winning essays. The winners are Spencer Terry of Tulsa, Oklahoma (secondary), and Carrie Snyder-Renfro of Oklahoma City, Oklahoma (postsecondary). The topic for secondary students is "How would you communicate the impact and importance of CTE related to your…

  7. Interview--Cliff Joseph: Art Therapist, Pioneer, Artist.

    ERIC Educational Resources Information Center

    Riley-Hiscox, Anna

    1997-01-01

    Interview of Cliff Joseph, one of the first African Americans to participate in the American Art Therapy Association and to become a registered art therapist and teacher. Details Joseph's impact on art therapy and cultural and social issues, as well as his relationships with other prominent art therapists. (MKA)

  8. Crawling-Onset Age Predicts Visual Cliff Avoidance in Infants.

    ERIC Educational Resources Information Center

    Richards, John E.; Rader, Nancy

    1981-01-01

    Two experiments tested the effects of crawling-onset age, amount of crawling experience, and testing age on avoidance of the deep side of a visual cliff apparatus by human infants. Crawling-onset age disciminated between infants because crawling during the tactile phase interferes with later visual control of locomotion. (Author/RD)

  9. Gravitational failure of sea cliffs in weakly lithified sediment

    USGS Publications Warehouse

    Hampton, M.A.

    2002-01-01

    Gravitational failure of sea cliffs eroded into weakly lithified sediment at several sites in California involves episodic stress-release fracturing and cantilevered block falls. The principal variables that influence the gravitational stability are tensional stresses generated during the release of horizontal confining stress and weakening of the sediment with increased saturation levels. Individual failures typically comprise less than a cubic meter of sediment, but large areas of a cliff face can be affected by sustained instability over a period of several days. Typically, only the outer meter or so of sediment is removed during a failure episode. In-place sediment saturation levels vary over time and space, generally being higher during the rainy season but moderate to high year-round. Laboratory direct-shear tests show that sediment cohesion decreases abruptly with increasing saturation level; the decrease is similar for all tested sediment if the cohesion is normalized by the maximum, dry-sediment cohesion. Large failures that extend over most or all of the height of the sea cliff are uncommon, but a few large wedge-shaped failures sometimes occur, as does separation of large blocks at sea cliff-gully intersections.

  10. 3D graphene oxide-polymer hydrogel: near-infrared light-triggered active scaffold for reversible cell capture and on-demand release.

    PubMed

    Li, Wen; Wang, Jiasi; Ren, Jinsong; Qu, Xiaogang

    2013-12-10

    An active cell scaffold based on a graphene-polymer hydrogel has been successfully fabricated. The macroporous hydrogel can efficiently capture cells not only through the bioadhesive ligand RGD but also through on-demand release of cells with an NIR light stimulus. The latter process shows better dynamic control over cells than traditional passive-hydrogel-based cell depots. PMID:24123218

  11. Biological activity profiles of 1alpha,25-dihydroxyvitamin D2, D3, D4, D7, and 24-epi-1alpha,25-dihydroxyvitamin D2.

    PubMed

    Tsugawa, N; Nakagawa, K; Kawamoto, Y; Tachibana, Y; Hayashi, T; Ozono, K; Okano, T

    1999-04-01

    We have synthesized several 1alpha,25-dihydroxyvitamin D [1alpha,25(OH)2D] derivatives and evaluated their biological activity in terms of their binding affinity for the vitamin D receptor (VDR) and vitamin D-binding protein (DBP), antiproliferative or differentiation-inducing effects on human promyelocytic leukemic HL-60 cells, and transcriptional activity on a rat 25-hydroxyvitamin D3-24-hydroxylase gene promoter, including two vitamin D-responsive elements (VDREs), and human osteocalcin gene promoter, including a VDRE in transfected human osteosarcoma MG-63 cells. Furthermore, human VDR- or retinoic acid X receptor alpha (RXR alpha)-mediated luciferase activities of the derivatives were also measured by a one-hybrid system in human epitheloid carcinoma, cervix HeLa cells and African green monkey kidney CV-1 cells. Binding affinity for VDR, bone-resorbing activity, antiproliferative and cell-differentiating effects, transactivation potencies on target genes and VDR- or RXR alpha-mediated gene regulations of 1alpha,25(OH)2D2 and 1alpha,25(OH)2D4 were almost comparable to the effects of 1alpha,25(OH)2D3 while 24-epi-1alpha,25(OH)2D2 and 1alpha,25(OH)2D7 were much less active than 1alpha,25(OH)2D3 in these respects. This is the first report concerning biological assessment of 1alpha,25(OH)2D2, 1alpha,25(OH)2D3, 1alpha,25(OH)2D4, 24-epi-1alpha,25(OH)2D2 and 1alpha,25(OH)2D7 at the molecular level, especially with regards to the structural differences at the 24R- or 24S-methyl group and a double bond between carbons 22 and 23 in the side chain of 1alpha,25(OH)2D derivatives. PMID:10328556

  12. Assessment of metal-assisted nucleophile activation in the hepatitis delta virus ribozyme from molecular simulation and 3D-RISM

    PubMed Central

    Radak, Brian K.; Lee, Tai-Sung; Harris, Michael E.

    2015-01-01

    The hepatitis delta virus ribozyme is an efficient catalyst of RNA 2′-O-transphosphorylation and has emerged as a key experimental system for identifying and characterizing fundamental features of RNA catalysis. Recent structural and biochemical data have led to a proposed mechanistic model whereby an active site Mg2+ ion facilitates deprotonation of the O2′ nucleophile, and a protonated cytosine residue (C75) acts as an acid to donate a proton to the O5′ leaving group as noted in a previous study. This model assumes that the active site Mg2+ ion forms an inner-sphere coordination with the O2′ nucleophile and a nonbridging oxygen of the scissile phosphate. These contacts, however, are not fully resolved in the crystal structure, and biochemical data are not able to unambiguously exclude other mechanistic models. In order to explore the feasibility of this model, we exhaustively mapped the free energy surfaces with different active site ion occupancies via quantum mechanical/molecular mechanical (QM/MM) simulations. We further incorporate a three-dimensional reference interaction site model for the solvated ion atmosphere that allows these calculations to consider not only the rate associated with the chemical steps, but also the probability of observing the system in the presumed active state with the Mg2+ ion bound. The QM/MM results predict that a pathway involving metal-assisted nucleophile activation is feasible based on the rate-controlling transition state barrier departing from the presumed metal-bound active state. However, QM/MM results for a similar pathway in the absence of Mg2+ are not consistent with experimental data, suggesting that a structural model in which the crystallographically determined Mg2+ is simply replaced with Na+ is likely incorrect. It should be emphasized, however, that these results hinge upon the assumption of the validity of the presumed Mg2+-bound starting state, which has not yet been definitively verified experimentally

  13. Volcano-tectonic implications of 3-D velocity structures derived from joint active and passive source tomography of the island of Hawaii

    USGS Publications Warehouse

    Park, J.; Morgan, J.K.; Zelt, C.A.; Okubo, P.G.

    2009-01-01

    We present a velocity model of the onshore and offshore regions around the southern part of the island of Hawaii, including southern Mauna Kea, southeastern Hualalai, and the active volcanoes of Mauna Loa, and Kilauea, and Loihi seamount. The velocity model was inverted from about 200,000 first-arrival traveltime picks of earthquakes and air gun shots recorded at the Hawaiian Volcano Observatory (HVO). Reconstructed volcanic structures of the island provide us with an improved understanding of the volcano-tectonic evolution of Hawaiian volcanoes and their interactions. The summits and upper rift zones of the active volcanoes are characterized by high-velocity materials, correlated with intrusive magma cumulates. These high-velocity materials often do not extend the full lengths of the rift zones, suggesting that rift zone intrusions may be spatially limited. Seismicity tends to be localized seaward of the most active intrusive bodies. Low-velocity materials beneath parts of the active rift zones of Kilauea and Mauna Loa suggest discontinuous rift zone intrusives, possibly due to the presence of a preexisting volcanic edifice, e.g., along Mauna Loa beneath Kilauea's southwest rift zone, or alternatively, removal of high-velocity materials by large-scale landsliding, e.g., along Mauna Loa's western flank. Both locations also show increased seismicity that may result from edifice interactions or reactivation of buried faults. New high-velocity regions are recognized and suggest the presence of buried, and in some cases, previously unknown rift zones, within the northwest flank of Mauna Loa, and the south flanks of Mauna Loa, Hualalai, and Mauna Kea. Copyright 2009 by the American Geophysical Union.

  14. 3D Imaging with Holographic Tomography

    NASA Astrophysics Data System (ADS)

    Sheppard, Colin J. R.; Kou, Shan Shan

    2010-04-01

    There are two main types of tomography that enable the 3D internal structures of objects to be reconstructed from scattered data. The commonly known computerized tomography (CT) give good results in the x-ray wavelength range where the filtered back-projection theorem and Radon transform can be used. These techniques rely on the Fourier projection-slice theorem where rays are considered to propagate straight through the object. Another type of tomography called `diffraction tomography' applies in applications in optics and acoustics where diffraction and scattering effects must be taken into account. The latter proves to be a more difficult problem, as light no longer travels straight through the sample. Holographic tomography is a popular way of performing diffraction tomography and there has been active experimental research on reconstructing complex refractive index data using this approach recently. However, there are two distinct ways of doing tomography: either by rotation of the object or by rotation of the illumination while fixing the detector. The difference between these two setups is intuitive but needs to be quantified. From Fourier optics and information transformation point of view, we use 3D transfer function analysis to quantitatively describe how spatial frequencies of the object are mapped to the Fourier domain. We first employ a paraxial treatment by calculating the Fourier transform of the defocused OTF. The shape of the calculated 3D CTF for tomography, by scanning the illumination in one direction only, takes on a form that we might call a 'peanut,' compared to the case of object rotation, where a diablo is formed, the peanut exhibiting significant differences and non-isotropy. In particular, there is a line singularity along one transverse direction. Under high numerical aperture conditions, the paraxial treatment is not accurate, and so we make use of 3D analytical geometry to calculate the behaviour in the non-paraxial case. This time, we

  15. Unlocking the scientific potential of complex 3D point cloud dataset : new classification and 3D comparison methods

    NASA Astrophysics Data System (ADS)

    Lague, D.; Brodu, N.; Leroux, J.

    2012-12-01

    Ground based lidar and photogrammetric techniques are increasingly used to track the evolution of natural surfaces in 3D at an unprecedented resolution and precision. The range of applications encompass many type of natural surfaces with different geometries and roughness characteristics (landslides, cliff erosion, river beds, bank erosion,....). Unravelling surface change in these contexts requires to compare large point clouds in 2D or 3D. The most commonly used method in geomorphology is based on a 2D difference of the gridded point clouds. Yet this is hardly adapted to many 3D natural environments such as rivers (with horizontal beds and vertical banks), while gridding complex rough surfaces is a complex task. On the other hand, tools allowing to perform 3D comparison are scarce and may require to mesh the point clouds which is difficult on rough natural surfaces. Moreover, existing 3D comparison tools do not provide an explicit calculation of confidence intervals that would factor in registration errors, roughness effects and instrument related position uncertainties. To unlock this problem, we developed the first algorithm combining a 3D measurement of surface change directly on point clouds with an estimate of spatially variable confidence intervals (called M3C2). The method has two steps : (1) surface normal estimation and orientation in 3D at a scale consistent with the local roughness ; (2) measurement of mean surface change along the normal direction with explicit calculation of a local confidence interval. Comparison with existing 3D methods based on a closest-point calculation demonstrates the higher precision of the M3C2 method when mm changes needs to be detected. The M3C2 method is also simple to use as it does not require surface meshing or gridding, and is not sensitive to missing data or change in point density. We also present a 3D classification tool (CANUPO) for vegetation removal based on a new geometrical measure: the multi

  16. 3D Printing of Graphene Aerogels.

    PubMed

    Zhang, Qiangqiang; Zhang, Feng; Medarametla, Sai Pradeep; Li, Hui; Zhou, Chi; Lin, Dong

    2016-04-01

    3D printing of a graphene aerogel with true 3D overhang structures is highlighted. The aerogel is fabricated by combining drop-on-demand 3D printing and freeze casting. The water-based GO ink is ejected and freeze-cast into designed 3D structures. The lightweight (<10 mg cm(-3) ) 3D printed graphene aerogel presents superelastic and high electrical conduction. PMID:26861680

  17. News and Views: Betelgeuse bubbles up dust; Hydrothermal activity on early asteroids; Is this a record? Galaxy evolution in 3D; LOFAR looks farther; IOPD makes plans

    NASA Astrophysics Data System (ADS)

    2011-08-01

    Red supergiant star Betelgeuse is surrounded by a vast halo of silicate and aluminium dust, visible in false colour in this infrared image from the European Southern Observatory's Very Large Telescope. This material may eventually form planets around a new star. Biochemical analysis of the Tagish Lake meteorites, some of the most pristine samples of carbonaceous chondrites known, suggests that much of the variation in organic matter between different meteorite samples can be ascribed to hydrothermal activity on meteorite parent bodies. European Southern Observatory astronomers have discovered the most distant quasar yet - and reckon it is one of the brightest objects in the early universe.

  18. ShowMe3D

    2012-01-05

    ShowMe3D is a data visualization graphical user interface specifically designed for use with hyperspectral image obtained from the Hyperspectral Confocal Microscope. The program allows the user to select and display any single image from a three dimensional hyperspectral image stack. By moving a slider control, the user can easily move between images of the stack. The user can zoom into any region of the image. The user can select any pixel or region from themore » displayed image and display the fluorescence spectrum associated with that pixel or region. The user can define up to 3 spectral filters to apply to the hyperspectral image and view the image as it would appear from a filter-based confocal microscope. The user can also obtain statistics such as intensity average and variance from selected regions.« less

  19. ShowMe3D

    SciTech Connect

    Sinclair, Michael B

    2012-01-05

    ShowMe3D is a data visualization graphical user interface specifically designed for use with hyperspectral image obtained from the Hyperspectral Confocal Microscope. The program allows the user to select and display any single image from a three dimensional hyperspectral image stack. By moving a slider control, the user can easily move between images of the stack. The user can zoom into any region of the image. The user can select any pixel or region from the displayed image and display the fluorescence spectrum associated with that pixel or region. The user can define up to 3 spectral filters to apply to the hyperspectral image and view the image as it would appear from a filter-based confocal microscope. The user can also obtain statistics such as intensity average and variance from selected regions.

  20. 3D Elastic Wavefield Tomography

    NASA Astrophysics Data System (ADS)

    Guasch, L.; Warner, M.; Stekl, I.; Umpleby, A.; Shah, N.

    2010-12-01

    Wavefield tomography, or waveform inversion, aims to extract the maximum information from seismic data by matching trace by trace the response of the solid earth to seismic waves using numerical modelling tools. Its first formulation dates from the early 80's, when Albert Tarantola developed a solid theoretical basis that is still used today with little change. Due to computational limitations, the application of the method to 3D problems has been unaffordable until a few years ago, and then only under the acoustic approximation. Although acoustic wavefield tomography is widely used, a complete solution of the seismic inversion problem requires that we account properly for the physics of wave propagation, and so must include elastic effects. We have developed a 3D tomographic wavefield inversion code that incorporates the full elastic wave equation. The bottle neck of the different implementations is the forward modelling algorithm that generates the synthetic data to be compared with the field seismograms as well as the backpropagation of the residuals needed to form the direction update of the model parameters. Furthermore, one or two extra modelling runs are needed in order to calculate the step-length. Our approach uses a FD scheme explicit time-stepping by finite differences that are 4th order in space and 2nd order in time, which is a 3D version of the one developed by Jean Virieux in 1986. We chose the time domain because an explicit time scheme is much less demanding in terms of memory than its frequency domain analogue, although the discussion of wich domain is more efficient still remains open. We calculate the parameter gradients for Vp and Vs by correlating the normal and shear stress wavefields respectively. A straightforward application would lead to the storage of the wavefield at all grid points at each time-step. We tackled this problem using two different approaches. The first one makes better use of resources for small models of dimension equal

  1. Assessment of accuracy of PET utilizing a 3-D phantom to simulate the activity distribution of ( sup 18 F)fluorodeoxyglucose uptake in the human brain

    SciTech Connect

    Hoffman, E.J.; Cutler, P.D.; Guerrero, T.M.; Digby, W.M.; Mazziotta, J.C. )

    1991-03-01

    A three-dimensional brain phantom has been developed to simulate the activity distributions found in human brain studies currently employed in positron emission tomography (PET). The phantom has a single contiguous chamber and utilizes thin layers of lucite to provide apparent relative concentrations of 5, 1, and 0 for gray matter, white matter, and CSF structures, respectively. The phantom and an ideal image set were created from the same set of data. Thus, the user has a basis for comparing measured images with an ideal set that allows a quantitative evaluation of errors in PET studies with an activity distribution similar to that found in patients. The phantom was employed in a study of the effect of deadtime and scatter on accuracy in quantitation on a current PET system. Deadtime correction factors were found to be significant (1.1-2.5) at count rates found in clinical studies. Deadtime correction techniques were found to be accurate to within 5%. Scatter in emission and attenuation correction data consistently caused 5-15% errors in quantitation, whereas correction for scatter in both types of data reduced errors in accuracy to less than 5%.

  2. 3D-catFISH: a system for automated quantitative three-dimensional compartmental analysis of temporal gene transcription activity imaged by fluorescence in situ hybridization.

    PubMed

    Chawla, Monica K; Lin, Gang; Olson, Kathy; Vazdarjanova, Almira; Burke, Sara N; McNaughton, Bruce L; Worley, Paul F; Guzowski, John F; Roysam, Badrinath; Barnes, Carol A

    2004-10-15

    Fluorescence in situ hybridization (FISH) of neural activity-regulated, immediate-early gene (IEG) expression provides a method of functional brain imaging with cellular resolution. This enables the identification, in one brain, of which specific principal neurons were active during each of two distinct behavioral epochs. The unprecedented potential of this differential method for large-scale analysis of functional neural circuits is limited, however, by the time-intensive nature of manual image analysis. A comprehensive software tool for processing three-dimensional, multi-spectral confocal image stacks is described which supports the automation of this analysis. Nuclei counterstained with conventional DNA dyes and FISH signals indicating the sub-cellular distribution of specific, IEG RNA species are imaged using different spectral channels. The DNA channel data are segmented into individual nuclei by a three-dimensional multi-step algorithm that corrects for depth-dependent attenuation, non-isotropic voxels, and imaging noise. Intra-nuclear and cytoplasmic FISH signals are associated spatially with the nuclear segmentation results to generate a detailed tabular/database and graphic representation. Here we present a comprehensive validation of data generated by the automated software against manual quantification by human experts on hippocampal and parietal cortical regions (96.5% concordance with multi-expert consensus). The high degree of reliability and accuracy suggests that the software will generalize well to multiple brain areas and eventually to large-scale brain analysis. PMID:15351517

  3. Supernova Remnant in 3-D

    NASA Technical Reports Server (NTRS)

    2009-01-01

    wavelengths. Since the amount of the wavelength shift is related to the speed of motion, one can determine how fast the debris are moving in either direction. Because Cas A is the result of an explosion, the stellar debris is expanding radially outwards from the explosion center. Using simple geometry, the scientists were able to construct a 3-D model using all of this information. A program called 3-D Slicer modified for astronomical use by the Astronomical Medicine Project at Harvard University in Cambridge, Mass. was used to display and manipulate the 3-D model. Commercial software was then used to create the 3-D fly-through.

    The blue filaments defining the blast wave were not mapped using the Doppler effect because they emit a different kind of light synchrotron radiation that does not emit light at discrete wavelengths, but rather in a broad continuum. The blue filaments are only a representation of the actual filaments observed at the blast wave.

    This visualization shows that there are two main components to this supernova remnant: a spherical component in the outer parts of the remnant and a flattened (disk-like) component in the inner region. The spherical component consists of the outer layer of the star that exploded, probably made of helium and carbon. These layers drove a spherical blast wave into the diffuse gas surrounding the star. The flattened component that astronomers were unable to map into 3-D prior to these Spitzer observations consists of the inner layers of the star. It is made from various heavier elements, not all shown in the visualization, such as oxygen, neon, silicon, sulphur, argon and iron.

    High-velocity plumes, or jets, of this material are shooting out from the explosion in the plane of the disk-like component mentioned above. Plumes of silicon appear in the northeast and southwest, while those of iron are seen in the southeast and north. These jets were already known and Doppler velocity measurements have been made for these

  4. Enhanced photocatalytic bacteriostatic activity towards Escherichia coli using 3D hierarchical microsphere BiOI/BiOBr under visible light irradiation.

    PubMed

    Wang, Ya; Lin, Li; Li, Fang; Chen, Liang; Chen, Donghui; Yang, Chongyang; Huang, Manhong

    2016-05-11

    A BiOI/BiOBr composite was successfully fabricated by a simple hydrothermal method. The composite was characterized by X-ray diffraction (XRD), UV-vis diffuse reflectance spectroscopy (UV-vis DRS), scanning electron microscopy (SEM), and high-resolution transmission electron microscopy (HRTEM). The BiOI/BiOBr composite exhibited enhanced photocatalytic bacteriostatic activity towards E. coli compared to the pure BiOI or BiOBr under visible light irradiation. The enhanced photocatalytic performance can be attributed to the improved separation efficiency of the photogenerated holes because of its heterojunction structure. In addition, the possible bacteriostatic mechanism of the BiOI/BiOBr composite under visible light irradiation is discussed. The hierarchical microsphere BiOI/BiOBr showed enhanced photocatalytic bacteriostasis towards Escherichia coli under visible light. PMID:27105324

  5. Genome-wide identification, 3D modeling, expression and enzymatic activity analysis of cell wall invertase gene family from cassava (Manihot esculenta Crantz).

    PubMed

    Yao, Yuan; Geng, Meng-Ting; Wu, Xiao-Hui; Liu, Jiao; Li, Rui-Mei; Hu, Xin-Wen; Guo, Jian-Chun

    2014-01-01

    The cell wall invertases play a crucial role on the sucrose metabolism in plant source and sink organs. In this research, six cell wall invertase genes (MeCWINV1-6) were cloned from cassava. All the MeCWINVs contain a putative signal peptide with a predicted extracellular location. The overall predicted structures of the MeCWINV1-6 are similar to AtcwINV1. Their N-terminus domain forms a β-propeller module and three conserved sequence domains (NDPNG, RDP and WECP(V)D), in which the catalytic residues are situated in these domains; while the C-terminus domain consists of a β-sandwich module. The predicted structure of Pro residue from the WECPD (MeCWINV1, 2, 5, and 6), and Val residue from the WECVD (MeCWINV3 and 4) are different. The activity of MeCWINV1 and 3 were higher than other MeCWINVs in leaves and tubers, which suggested that sucrose was mainly catalyzed by the MeCWINV1 and 3 in the apoplastic space of cassava source and sink organs. The transcriptional levels of all the MeCWINVs and their enzymatic activity were lower in tubers than in leaves at all the stages during the cassava tuber development. It suggested that the major role of the MeCWINVs was on the regulation of carbon exportation from source leaves, and the ratio of sucrose to hexose in the apoplasts; the role of these enzymes on the sucrose unloading to tuber was weaker. PMID:24786092

  6. The Europa Imaging System (EIS): High-Resolution, 3-D Insight into Europa's Geology, Ice Shell, and Potential for Current Activity

    NASA Astrophysics Data System (ADS)

    Turtle, E. P.; McEwen, A. S.; Collins, G. C.; Fletcher, L. N.; Hansen, C. J.; Hayes, A.; Hurford, T., Jr.; Kirk, R. L.; Barr, A.; Nimmo, F.; Patterson, G.; Quick, L. C.; Soderblom, J. M.; Thomas, N.

    2015-12-01

    The Europa Imaging System will transform our understanding of Europa through global decameter-scale coverage, three-dimensional maps, and unprecedented meter-scale imaging. EIS combines narrow-angle and wide-angle cameras (NAC and WAC) designed to address high-priority Europa science and reconnaissance goals. It will: (A) Characterize the ice shell by constraining its thickness and correlating surface features with subsurface structures detected by ice penetrating radar; (B) Constrain formation processes of surface features and the potential for current activity by characterizing endogenic structures, surface units, global cross-cutting relationships, and relationships to Europa's subsurface structure, and by searching for evidence of recent activity, including potential plumes; and (C) Characterize scientifically compelling landing sites and hazards by determining the nature of the surface at scales relevant to a potential lander. The NAC provides very high-resolution, stereo reconnaissance, generating 2-km-wide swaths at 0.5-m pixel scale from 50-km altitude, and uses a gimbal to enable independent targeting. NAC observations also include: near-global (>95%) mapping of Europa at ≤50-m pixel scale (to date, only ~14% of Europa has been imaged at ≤500 m/pixel, with best pixel scale 6 m); regional and high-resolution stereo imaging at <1-m/pixel; and high-phase-angle observations for plume searches. The WAC is designed to acquire pushbroom stereo swaths along flyby ground-tracks, generating digital topographic models with 32-m spatial scale and 4-m vertical precision from 50-km altitude. These data support characterization of cross-track clutter for radar sounding. The WAC also performs pushbroom color imaging with 6 broadband filters (350-1050 nm) to map surface units and correlations with geologic features and topography. EIS will provide comprehensive data sets essential to fulfilling the goal of exploring Europa to investigate its habitability and perform

  7. The Europa Imaging System (EIS): High-Resolution, 3-D Insight into Europa's Geology, Ice Shell, and Potential for Current Activity

    NASA Astrophysics Data System (ADS)

    Turtle, E. P.; McEwen, A. S.; Collins, G. C.; Fletcher, L. N.; Hansen, C. J.; Hayes, A.; Hurford, T., Jr.; Kirk, R. L.; Barr, A.; Nimmo, F.; Patterson, G.; Quick, L. C.; Soderblom, J. M.; Thomas, N.

    2014-12-01

    The Europa Imaging System will transform our understanding of Europa through global decameter-scale coverage, three-dimensional maps, and unprecedented meter-scale imaging. EIS combines narrow-angle and wide-angle cameras (NAC and WAC) designed to address high-priority Europa science and reconnaissance goals. It will: (A) Characterize the ice shell by constraining its thickness and correlating surface features with subsurface structures detected by ice penetrating radar; (B) Constrain formation processes of surface features and the potential for current activity by characterizing endogenic structures, surface units, global cross-cutting relationships, and relationships to Europa's subsurface structure, and by searching for evidence of recent activity, including potential plumes; and (C) Characterize scientifically compelling landing sites and hazards by determining the nature of the surface at scales relevant to a potential lander. The NAC provides very high-resolution, stereo reconnaissance, generating 2-km-wide swaths at 0.5-m pixel scale from 50-km altitude, and uses a gimbal to enable independent targeting. NAC observations also include: near-global (>95%) mapping of Europa at ≤50-m pixel scale (to date, only ~14% of Europa has been imaged at ≤500 m/pixel, with best pixel scale 6 m); regional and high-resolution stereo imaging at <1-m/pixel; and high-phase-angle observations for plume searches. The WAC is designed to acquire pushbroom stereo swaths along flyby ground-tracks, generating digital topographic models with 32-m spatial scale and 4-m vertical precision from 50-km altitude. These data support characterization of cross-track clutter for radar sounding. The WAC also performs pushbroom color imaging with 6 broadband filters (350-1050 nm) to map surface units and correlations with geologic features and topography. EIS will provide comprehensive data sets essential to fulfilling the goal of exploring Europa to investigate its habitability and perform

  8. Rough surface effects on active and passive microwave remote sensing of soil moisture at L-band using 3D fast solution of Maxwell's equations

    NASA Astrophysics Data System (ADS)

    Wang, Haogang; Liao, Tien-Hao; Shi, Jiancheng; Yu, Zherui

    2014-11-01

    The forthcoming Water Cycle Observation Mission (WCOM) is to understand the water cycle system among land, atmosphere, and ocean. In both active and passive microwave remote sensing of soil moisture, the surface roughness plays an important role. Electromagnetic models of roughness provide tables of emissivities and backscattering coefficients that can be used to retrieve soil moisture. In this paper, a fast and accurate three dimensional solution of Maxwell's equations is developed and employed to solve rough soil surface scattering problem at L-band. The algorithm combines QR Pre-Ranked Multilevel UV(MLUV) factorization and Hierarchical Fast Far Field Approximation. It is implemented using OpenMP interface for fast parallel calculation. In this algorithm, 1) QR based rank predetermined algorithm is derived to further compress the UV matrix pairs obtained using coarse-coarse sampling; 2) at the finer levels, MLUV is used straightforwardly to factorize the interactions between groups, while at the coarsest level, interactions between groups in the interaction list are calculated using an elegantly derived Hierarchical Fast Far Field Approximation (HFAFFA) to accelerate the calculation of interactions between large groups while keeping the accuracy of this approximation; 3) OpenMP interface is used to parallelize this new algorithm. Numerical results including the incoherent bistatic scattering coefficients and the emissivity demonstrate the efficiency of this method.

  9. Met.3D - a new open-source tool for interactive 3D visualization of ensemble weather forecasts

    NASA Astrophysics Data System (ADS)

    Rautenhaus, Marc; Kern, Michael; Schäfler, Andreas; Westermann, Rüdiger

    2015-04-01

    We introduce Met.3D, a new open-source tool for the interactive 3D visualization of numerical ensemble weather predictions. The tool has been developed to support weather forecasting during aircraft-based atmospheric field campaigns, however, is applicable to further forecasting, research and teaching activities. Our work approaches challenging topics related to the visual analysis of numerical atmospheric model output -- 3D visualisation, ensemble visualization, and how both can be used in a meaningful way suited to weather forecasting. Met.3D builds a bridge from proven 2D visualization methods commonly used in meteorology to 3D visualization by combining both visualization types in a 3D context. It implements methods that address the issue of spatial perception in the 3D view as well as approaches to using the ensemble in order to assess forecast uncertainty. Interactivity is key to the Met.3D approach. The tool uses modern graphics hardware technology to achieve interactive visualization of present-day numerical weather prediction datasets on standard consumer hardware. Met.3D supports forecast data from the European Centre for Medium Range Weather Forecasts and operates directly on ECMWF hybrid sigma-pressure level grids. In this presentation, we provide an overview of the software --illustrated with short video examples--, and give information on its availability.

  10. 3-D visualization of ensemble weather forecasts - Part 1: The visualization tool Met.3D (version 1.0)

    NASA Astrophysics Data System (ADS)

    Rautenhaus, M.; Kern, M.; Schäfler, A.; Westermann, R.

    2015-02-01

    We present Met.3D, a new open-source tool for the interactive 3-D visualization of numerical ensemble weather predictions. The tool has been developed to support weather forecasting during aircraft-based atmospheric field campaigns, however, is applicable to further forecasting, research and teaching activities. Our work approaches challenging topics related to the visual analysis of numerical atmospheric model output - 3-D visualization, ensemble visualization, and how both can be used in a meaningful way suited to weather forecasting. Met.3D builds a bridge from proven 2-D visualization methods commonly used in meteorology to 3-D visualization by combining both visualization types in a 3-D context. We address the issue of spatial perception in the 3-D view and present approaches to using the ensemble to allow the user to assess forecast uncertainty. Interactivity is key to our approach. Met.3D uses modern graphics technology to achieve interactive visualization on standard consumer hardware. The tool supports forecast data from the European Centre for Medium Range Weather Forecasts and can operate directly on ECMWF hybrid sigma-pressure level grids. We describe the employed visualization algorithms, and analyse the impact of the ECMWF grid topology on computing 3-D ensemble statistical quantitites. Our techniques are demonstrated with examples from the T-NAWDEX-Falcon 2012 campaign.

  11. Amazing Space: Explanations, Investigations, & 3D Visualizations

    NASA Astrophysics Data System (ADS)

    Summers, Frank

    2011-05-01

    The Amazing Space website is STScI's online resource for communicating Hubble discoveries and other astronomical wonders to students and teachers everywhere. Our team has developed a broad suite of materials, readings, activities, and visuals that are not only engaging and exciting, but also standards-based and fully supported so that they can be easily used within state and national curricula. These products include stunning imagery, grade-level readings, trading card games, online interactives, and scientific visualizations. We are currently exploring the potential use of stereo 3D in astronomy education.

  12. Supernova Remnant in 3-D

    NASA Technical Reports Server (NTRS)

    2009-01-01

    wavelengths. Since the amount of the wavelength shift is related to the speed of motion, one can determine how fast the debris are moving in either direction. Because Cas A is the result of an explosion, the stellar debris is expanding radially outwards from the explosion center. Using simple geometry, the scientists were able to construct a 3-D model using all of this information. A program called 3-D Slicer modified for astronomical use by the Astronomical Medicine Project at Harvard University in Cambridge, Mass. was used to display and manipulate the 3-D model. Commercial software was then used to create the 3-D fly-through.

    The blue filaments defining the blast wave were not mapped using the Doppler effect because they emit a different kind of light synchrotron radiation that does not emit light at discrete wavelengths, but rather in a broad continuum. The blue filaments are only a representation of the actual filaments observed at the blast wave.

    This visualization shows that there are two main components to this supernova remnant: a spherical component in the outer parts of the remnant and a flattened (disk-like) component in the inner region. The spherical component consists of the outer layer of the star that exploded, probably made of helium and carbon. These layers drove a spherical blast wave into the diffuse gas surrounding the star. The flattened component that astronomers were unable to map into 3-D prior to these Spitzer observations consists of the inner layers of the star. It is made from various heavier elements, not all shown in the visualization, such as oxygen, neon, silicon, sulphur, argon and iron.

    High-velocity plumes, or jets, of this material are shooting out from the explosion in the plane of the disk-like component mentioned above. Plumes of silicon appear in the northeast and southwest, while those of iron are seen in the southeast and north. These jets were already known and Doppler velocity measurements have been made for these

  13. 3D multiplexed immunoplasmonics microscopy

    NASA Astrophysics Data System (ADS)

    Bergeron, Éric; Patskovsky, Sergiy; Rioux, David; Meunier, Michel

    2016-07-01

    Selective labelling, identification and spatial distribution of cell surface biomarkers can provide important clinical information, such as distinction between healthy and diseased cells, evolution of a disease and selection of the optimal patient-specific treatment. Immunofluorescence is the gold standard for efficient detection of biomarkers expressed by cells. However, antibodies (Abs) conjugated to fluorescent dyes remain limited by their photobleaching, high sensitivity to the environment, low light intensity, and wide absorption and emission spectra. Immunoplasmonics is a novel microscopy method based on the visualization of Abs-functionalized plasmonic nanoparticles (fNPs) targeting cell surface biomarkers. Tunable fNPs should provide higher multiplexing capacity than immunofluorescence since NPs are photostable over time, strongly scatter light at their plasmon peak wavelengths and can be easily functionalized. In this article, we experimentally demonstrate accurate multiplexed detection based on the immunoplasmonics approach. First, we achieve the selective labelling of three targeted cell surface biomarkers (cluster of differentiation 44 (CD44), epidermal growth factor receptor (EGFR) and voltage-gated K+ channel subunit KV1.1) on human cancer CD44+ EGFR+ KV1.1+ MDA-MB-231 cells and reference CD44- EGFR- KV1.1+ 661W cells. The labelling efficiency with three stable specific immunoplasmonics labels (functionalized silver nanospheres (CD44-AgNSs), gold (Au) NSs (EGFR-AuNSs) and Au nanorods (KV1.1-AuNRs)) detected by reflected light microscopy (RLM) is similar to the one with immunofluorescence. Second, we introduce an improved method for 3D localization and spectral identification of fNPs based on fast z-scanning by RLM with three spectral filters corresponding to the plasmon peak wavelengths of the immunoplasmonics labels in the cellular environment (500 nm for 80 nm AgNSs, 580 nm for 100 nm AuNSs and 700 nm for 40 nm × 92 nm AuNRs). Third, the developed

  14. Investigation of the deep crustal structure and magmatic activity at the NW Hellenic Volcanic Arc with 3-D aeromagnetic inversion and seimotectonic analysis.

    NASA Astrophysics Data System (ADS)

    Efstathiou, Angeliki; Tzanis, Andreas; Chailas, Stylianos; Stamatakis, Michael

    2013-04-01

    We report the results of a joint analysis of geophysical (aeromagnetic) and seismotectonic data, applied to the investigation of the deep structure, magmatic activity and geothermal potential of the north-western stretches of the Hellenic Volcanic Arc (HVA). The HVA is usually considered to be a single arcuate entity stretching from Sousaki (near Corinth) at the NW, to Nisyros Island at the SE. However, different types of and their ages indicate the presence of two different volcanic groups. Our study focuses on the northern part of the west (older) volcanic group and includes the Crommyonian (Sousaki) volcanic field at the west end of Megaris peninsula (east margin on the contemporary Corinth Rift), the Aegina and Methana volcanic complex at the Saronic Gulf, where typical Quaternary calc-alkaline volcanics predominate, and the Argolid peninsula to the south and south-west. In addition to the rocks associated with Quaternary volcanism, the study area includes a series of Mesozoic ultramafic (ophiolitic) outcrops at the Megaris peninsula, to the north and north-east of the Crommyonian volcanic field, as well as throughout the Argolid. A major deep structural and tectonic feature of the study area, and one with profound influence on crustal deformation and the evolution of rapidly deforming extensional structures like the Corinth Rift and the Saronic Gulf, is the local geometry and dynamics of the African oceanic crust subducting beneath the Aegean plate. Locally, the subducting slab has a NNW strike and ENE plunge, with the dip angle changing rapidly (steepening) approx. beneath the Argolid. The aeromagnetic data was extracted from the recently (re)compiled aeromagnetic map of Greece (Chailas et al, 2010) and was inverted with the UBC-GIF magnetic inversion suite (Li and Oldenburg, 1996). The inversion included rigorous geological constraints introduced by means of numerous in-situ magnetic susceptibility measurements. The inversion has imaged several isolated

  15. Investigation of the deep crustal structure and magmatic activity at the NW Hellenic Volcanic Arc with 3-D aeromagnetic inversion and seimotectonic analysis.

    NASA Astrophysics Data System (ADS)

    Efstathiou, Angeliki; Tzanis, Andreas; Chailas, Stylianos; Stamatakis, Michael

    2013-04-01

    We report the results of a joint analysis of geophysical (aeromagnetic) and seismotectonic data, applied to the investigation of the deep structure, magmatic activity and geothermal potential of the north-western stretches of the Hellenic Volcanic Arc (HVA). The HVA is usually considered to be a single arcuate entity stretching from Sousaki (near Corinth) at the NW, to Nisyros Island at the SE. However, different types of and their ages indicate the presence of two different volcanic groups. Our study focuses on the northern part of the west (older) volcanic group and includes the Crommyonian (Sousaki) volcanic field at the west end of Megaris peninsula (east margin on the contemporary Corinth Rift), the Aegina and Methana volcanic complex at the Saronic Gulf, where typical Quaternary calc-alkaline volcanics predominate, and the Argolid peninsula to the south and south-west. In addition to the rocks associated with Quaternary volcanism, the study area includes a series of Mesozoic ultramafic (ophiolitic) outcrops at the Megaris peninsula, to the north and north-east of the Crommyonian volcanic field, as well as throughout the Argolid. A major deep structural and tectonic feature of the study area, and one with profound influence on crustal deformation and the evolution of rapidly deforming extensional structures like the Corinth Rift and the Saronic Gulf, is the local geometry and dynamics of the African oceanic crust subducting beneath the Aegean plate. Locally, the subducting slab has a NNW strike and ENE plunge, with the dip angle changing rapidly (steepening) approx. beneath the Argolid. The aeromagnetic data was extracted from the recently (re)compiled aeromagnetic map of Greece (Chailas et al, 2010) and was inverted with the UBC-GIF magnetic inversion suite (Li and Oldenburg, 1996). The inversion included rigorous geological constraints introduced by means of numerous in-situ magnetic susceptibility measurements. The inversion has imaged several isolated

  16. NIF Ignition Target 3D Point Design

    SciTech Connect

    Jones, O; Marinak, M; Milovich, J; Callahan, D

    2008-11-05

    We have developed an input file for running 3D NIF hohlraums that is optimized such that it can be run in 1-2 days on parallel computers. We have incorporated increasing levels of automation into the 3D input file: (1) Configuration controlled input files; (2) Common file for 2D and 3D, different types of capsules (symcap, etc.); and (3) Can obtain target dimensions, laser pulse, and diagnostics settings automatically from NIF Campaign Management Tool. Using 3D Hydra calculations to investigate different problems: (1) Intrinsic 3D asymmetry; (2) Tolerance to nonideal 3D effects (e.g. laser power balance, pointing errors); and (3) Synthetic diagnostics.

  17. 3D multiplexed immunoplasmonics microscopy.

    PubMed

    Bergeron, Éric; Patskovsky, Sergiy; Rioux, David; Meunier, Michel

    2016-07-21

    Selective labelling, identification and spatial distribution of cell surface biomarkers can provide important clinical information, such as distinction between healthy and diseased cells, evolution of a disease and selection of the optimal patient-specific treatment. Immunofluorescence is the gold standard for efficient detection of biomarkers expressed by cells. However, antibodies (Abs) conjugated to fluorescent dyes remain limited by their photobleaching, high sensitivity to the environment, low light intensity, and wide absorption and emission spectra. Immunoplasmonics is a novel microscopy method based on the visualization of Abs-functionalized plasmonic nanoparticles (fNPs) targeting cell surface biomarkers. Tunable fNPs should provide higher multiplexing capacity than immunofluorescence since NPs are photostable over time, strongly scatter light at their plasmon peak wavelengths and can be easily functionalized. In this article, we experimentally demonstrate accurate multiplexed detection based on the immunoplasmonics approach. First, we achieve the selective labelling of three targeted cell surface biomarkers (cluster of differentiation 44 (CD44), epidermal growth factor receptor (EGFR) and voltage-gated K(+) channel subunit KV1.1) on human cancer CD44(+) EGFR(+) KV1.1(+) MDA-MB-231 cells and reference CD44(-) EGFR(-) KV1.1(+) 661W cells. The labelling efficiency with three stable specific immunoplasmonics labels (functionalized silver nanospheres (CD44-AgNSs), gold (Au) NSs (EGFR-AuNSs) and Au nanorods (KV1.1-AuNRs)) detected by reflected light microscopy (RLM) is similar to the one with immunofluorescence. Second, we introduce an improved method for 3D localization and spectral identification of fNPs based on fast z-scanning by RLM with three spectral filters corresponding to the plasmon peak wavelengths of the immunoplasmonics labels in the cellular environment (500 nm for 80 nm AgNSs, 580 nm for 100 nm AuNSs and 700 nm for 40 nm × 92 nm AuNRs). Third

  18. Advanced Data Visualization in Astrophysics: The X3D Pathway

    NASA Astrophysics Data System (ADS)

    Vogt, Frédéric P. A.; Owen, Chris I.; Verdes-Montenegro, Lourdes; Borthakur, Sanchayeeta

    2016-02-01

    Most modern astrophysical data sets are multi-dimensional; a characteristic that can nowadays generally be conserved and exploited scientifically during the data reduction/simulation and analysis cascades. However, the same multi-dimensional data sets are systematically cropped, sliced, and/or projected to printable two-dimensional diagrams at the publication stage. In this article, we introduce the concept of the “X3D pathway” as a mean of simplifying and easing the access to data visualization and publication via three-dimensional (3D) diagrams. The X3D pathway exploits the facts that (1) the X3D 3D file format lies at the center of a product tree that includes interactive HTML documents, 3D printing, and high-end animations, and (2) all high-impact-factor and peer-reviewed journals in astrophysics are now published (some exclusively) online. We argue that the X3D standard is an ideal vector for sharing multi-dimensional data sets because it provides direct access to a range of different data visualization techniques, is fully open source, and is a well-defined standard from the International Organization for Standardization. Unlike other earlier propositions to publish multi-dimensional data sets via 3D diagrams, the X3D pathway is not tied to specific software (prone to rapid and unexpected evolution), but instead is compatible with a range of open-source software already in use by our community. The interactive HTML branch of the X3D pathway is also actively supported by leading peer-reviewed journals in the field of astrophysics. Finally, this article provides interested readers with a detailed set of practical astrophysical examples designed to act as a stepping stone toward the implementation of the X3D pathway for any other data set.

  19. 3D Kitaev spin liquids

    NASA Astrophysics Data System (ADS)

    Hermanns, Maria

    The Kitaev honeycomb model has become one of the archetypal spin models exhibiting topological phases of matter, where the magnetic moments fractionalize into Majorana fermions interacting with a Z2 gauge field. In this talk, we discuss generalizations of this model to three-dimensional lattice structures. Our main focus is the metallic state that the emergent Majorana fermions form. In particular, we discuss the relation of the nature of this Majorana metal to the details of the underlying lattice structure. Besides (almost) conventional metals with a Majorana Fermi surface, one also finds various realizations of Dirac semi-metals, where the gapless modes form Fermi lines or even Weyl nodes. We introduce a general classification of these gapless quantum spin liquids using projective symmetry analysis. Furthermore, we briefly outline why these Majorana metals in 3D Kitaev systems provide an even richer variety of Dirac and Weyl phases than possible for electronic matter and comment on possible experimental signatures. Work done in collaboration with Kevin O'Brien and Simon Trebst.

  20. Yogi the rock - 3D

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Yogi, a rock taller than rover Sojourner, is the subject of this image, taken in stereo by the deployed Imager for Mars Pathfinder (IMP) on Sol 3. 3D glasses are necessary to identify surface detail. The soil in the foreground has been the location of multiple soil mechanics experiments performed by Sojourner's cleated wheels. Pathfinder scientists were able to control the force inflicted on the soil beneath the rover's wheels, giving them insight into the soil's mechanical properties. The soil mechanics experiments were conducted after this image was taken.

    Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

    Click below to see the left and right views individually. [figure removed for brevity, see original site] Left [figure removed for brevity, see original site] Right

  1. 3D ultrafast laser scanner

    NASA Astrophysics Data System (ADS)

    Mahjoubfar, A.; Goda, K.; Wang, C.; Fard, A.; Adam, J.; Gossett, D. R.; Ayazi, A.; Sollier, E.; Malik, O.; Chen, E.; Liu, Y.; Brown, R.; Sarkhosh, N.; Di Carlo, D.; Jalali, B.

    2013-03-01

    Laser scanners are essential for scientific research, manufacturing, defense, and medical practice. Unfortunately, often times the speed of conventional laser scanners (e.g., galvanometric mirrors and acousto-optic deflectors) falls short for many applications, resulting in motion blur and failure to capture fast transient information. Here, we present a novel type of laser scanner that offers roughly three orders of magnitude higher scan rates than conventional methods. Our laser scanner, which we refer to as the hybrid dispersion laser scanner, performs inertia-free laser scanning by dispersing a train of broadband pulses both temporally and spatially. More specifically, each broadband pulse is temporally processed by time stretch dispersive Fourier transform and further dispersed into space by one or more diffractive elements such as prisms and gratings. As a proof-of-principle demonstration, we perform 1D line scans at a record high scan rate of 91 MHz and 2D raster scans and 3D volumetric scans at an unprecedented scan rate of 105 kHz. The method holds promise for a broad range of scientific, industrial, and biomedical applications. To show the utility of our method, we demonstrate imaging, nanometer-resolved surface vibrometry, and high-precision flow cytometry with real-time throughput that conventional laser scanners cannot offer due to their low scan rates.

  2. Crowdsourcing Based 3d Modeling

    NASA Astrophysics Data System (ADS)

    Somogyi, A.; Barsi, A.; Molnar, B.; Lovas, T.

    2016-06-01

    Web-based photo albums that support organizing and viewing the users' images are widely used. These services provide a convenient solution for storing, editing and sharing images. In many cases, the users attach geotags to the images in order to enable using them e.g. in location based applications on social networks. Our paper discusses a procedure that collects open access images from a site frequently visited by tourists. Geotagged pictures showing the image of a sight or tourist attraction are selected and processed in photogrammetric processing software that produces the 3D model of the captured object. For the particular investigation we selected three attractions in Budapest. To assess the geometrical accuracy, we used laser scanner and DSLR as well as smart phone photography to derive reference values to enable verifying the spatial model obtained from the web-album images. The investigation shows how detailed and accurate models could be derived applying photogrammetric processing software, simply by using images of the community, without visiting the site.

  3. 3D multiplexed immunoplasmonics microscopy

    NASA Astrophysics Data System (ADS)

    Bergeron, Éric; Patskovsky, Sergiy; Rioux, David; Meunier, Michel

    2016-07-01

    Selective labelling, identification and spatial distribution of cell surface biomarkers can provide important clinical information, such as distinction between healthy and diseased cells, evolution of a disease and selection of the optimal patient-specific treatment. Immunofluorescence is the gold standard for efficient detection of biomarkers expressed by cells. However, antibodies (Abs) conjugated to fluorescent dyes remain limited by their photobleaching, high sensitivity to the environment, low light intensity, and wide absorption and emission spectra. Immunoplasmonics is a novel microscopy method based on the visualization of Abs-functionalized plasmonic nanoparticles (fNPs) targeting cell surface biomarkers. Tunable fNPs should provide higher multiplexing capacity than immunofluorescence since NPs are photostable over time, strongly scatter light at their plasmon peak wavelengths and can be easily functionalized. In this article, we experimentally demonstrate accurate multiplexed detection based on the immunoplasmonics approach. First, we achieve the selective labelling of three targeted cell surface biomarkers (cluster of differentiation 44 (CD44), epidermal growth factor receptor (EGFR) and voltage-gated K+ channel subunit KV1.1) on human cancer CD44+ EGFR+ KV1.1+ MDA-MB-231 cells and reference CD44- EGFR- KV1.1+ 661W cells. The labelling efficiency with three stable specific immunoplasmonics labels (functionalized silver nanospheres (CD44-AgNSs), gold (Au) NSs (EGFR-AuNSs) and Au nanorods (KV1.1-AuNRs)) detected by reflected light microscopy (RLM) is similar to the one with immunofluorescence. Second, we introduce an improved method for 3D localization and spectral identification of fNPs based on fast z-scanning by RLM with three spectral filters corresponding to the plasmon peak wavelengths of the immunoplasmonics labels in the cellular environment (500 nm for 80 nm AgNSs, 580 nm for 100 nm AuNSs and 700 nm for 40 nm × 92 nm AuNRs). Third, the developed

  4. Hot spring deposits on a cliff face: A case study from Jifei, Yunnan Province, China

    NASA Astrophysics Data System (ADS)

    Jones, Brian; Peng, Xiaotong

    2014-04-01

    A cliff face in the Jifei karst area, southwest China, is covered by a spectacular succession of precipitates that formed from the hot spring water that once flowed down its surface. This layered succession is formed of aragonite layers that are formed largely of “fountain dendrites”, calcite layers that are formed mostly of “cone dendrites”, and microlaminated layers that contain numerous microbes and extracellular polymeric substances (EPS). Many of the aragonite crystals are hollow due to preferential dissolution of their cores. The calcite cone dendrites are commonly covered with biofilms, reticulate Si-Mg coatings, and other precipitates. The microbial layers include dodecahedral calcite crystals and accessory minerals that include opal-A, amorphous Si-Mg coatings, trona, barite, potassium sulfate crystals, mirabillite, and gaylussite. Interpretation of the δ18O(calcite) and δ18O(aragonite) indicates precipitation from water with a temperature of 54 to 66 °C. The active hot spring at the top of the cliff presently ejects water at a temperature of 65 °C. Layers, 1 mm to 6 cm thick, record temporal changes in the fluids from which the precipitates formed. This succession is not, however, formed of recurring cycles that can be linked to diurnal or seasonal changes in the local climate. Indeed, it appears that the climatic contrast between the wet season and the dry season had little impact on precipitation from the spring waters that flowed down the cliff face. Integration of currently available evidence suggests that the primary driving force was aperiodic changes in the CO2 content of the spring waters because that seems to be the prime control on the saturation levels that underpinned precipitation of the calcite and aragonite as well as the dissolution of the aragonite. Such variations in the CO2 content of the spring water were probably due to changes that took place in the subterranean plumbing system of the spring.

  5. Distributed 3D Information Visualization - Towards Integration of the Dynamic 3D Graphics and Web Services

    NASA Astrophysics Data System (ADS)

    Vucinic, Dean; Deen, Danny; Oanta, Emil; Batarilo, Zvonimir; Lacor, Chris

    This paper focuses on visualization and manipulation of graphical content in distributed network environments. The developed graphical middleware and 3D desktop prototypes were specialized for situational awareness. This research was done in the LArge Scale COllaborative decision support Technology (LASCOT) project, which explored and combined software technologies to support human-centred decision support system for crisis management (earthquake, tsunami, flooding, airplane or oil-tanker incidents, chemical, radio-active or other pollutants spreading, etc.). The performed state-of-the-art review did not identify any publicly available large scale distributed application of this kind. Existing proprietary solutions rely on the conventional technologies and 2D representations. Our challenge was to apply the "latest" available technologies, such Java3D, X3D and SOAP, compatible with average computer graphics hardware. The selected technologies are integrated and we demonstrate: the flow of data, which originates from heterogeneous data sources; interoperability across different operating systems and 3D visual representations to enhance the end-users interactions.

  6. Creating 3D visualizations of MRI data: A brief guide

    PubMed Central

    Madan, Christopher R.

    2015-01-01

    While magnetic resonance imaging (MRI) data is itself 3D, it is often difficult to adequately present the results papers and slides in 3D. As a result, findings of MRI studies are often presented in 2D instead. A solution is to create figures that include perspective and can convey 3D information; such figures can sometimes be produced by standard functional magnetic resonance imaging (fMRI) analysis packages and related specialty programs. However, many options cannot provide functionality such as visualizing activation clusters that are both cortical and subcortical (i.e., a 3D glass brain), the production of several statistical maps with an identical perspective in the 3D rendering, or animated renderings. Here I detail an approach for creating 3D visualizations of MRI data that satisfies all of these criteria. Though a 3D ‘glass brain’ rendering can sometimes be difficult to interpret, they are useful in showing a more overall representation of the results, whereas the traditional slices show a more local view. Combined, presenting both 2D and 3D representations of MR images can provide a more comprehensive view of the study’s findings. PMID:26594340

  7. 3D printed quantum dot light-emitting diodes.

    PubMed

    Kong, Yong Lin; Tamargo, Ian A; Kim, Hyoungsoo; Johnson, Blake N; Gupta, Maneesh K; Koh, Tae-Wook; Chin, Huai-An; Steingart, Daniel A; Rand, Barry P; McAlpine, Michael C

    2014-12-10

    Developing the ability to 3D print various classes of materials possessing distinct properties could enable the freeform generation of active electronics in unique functional, interwoven architectures. Achieving seamless integration of diverse materials with 3D printing is a significant challenge that requires overcoming discrepancies in material properties in addition to ensuring that all the materials are compatible with the 3D printing process. To date, 3D printing has been limited to specific plastics, passive conductors, and a few biological materials. Here, we show that diverse classes of materials can be 3D printed and fully integrated into device components with active properties. Specifically, we demonstrate the seamless interweaving of five different materials, including (1) emissive semiconducting inorganic nanoparticles, (2) an elastomeric matrix, (3) organic polymers as charge transport layers, (4) solid and liquid metal leads, and (5) a UV-adhesive transparent substrate layer. As a proof of concept for demonstrating the integrated functionality of these materials, we 3D printed quantum dot-based light-emitting diodes (QD-LEDs) that exhibit pure and tunable color emission properties. By further incorporating the 3D scanning of surface topologies, we demonstrate the ability to conformally print devices onto curvilinear surfaces, such as contact lenses. Finally, we show that novel architectures that are not easily accessed using standard microfabrication techniques can be constructed, by 3D printing a 2 × 2 × 2 cube of encapsulated LEDs, in which every component of the cube and electronics are 3D printed. Overall, these results suggest that 3D printing is more versatile than has been demonstrated to date and is capable of integrating many distinct classes of materials. PMID:25360485

  8. 3D-printed mechanochromic materials.

    PubMed

    Peterson, Gregory I; Larsen, Michael B; Ganter, Mark A; Storti, Duane W; Boydston, Andrew J

    2015-01-14

    We describe the preparation and characterization of photo- and mechanochromic 3D-printed structures using a commercial fused filament fabrication printer. Three spiropyran-containing poly(ε-caprolactone) (PCL) polymers were each filamentized and used to print single- and multicomponent tensile testing specimens that would be difficult, if not impossible, to prepare using traditional manufacturing techniques. It was determined that the filament production and printing process did not degrade the spiropyran units or polymer chains and that the mechanical properties of the specimens prepared with the custom filament were in good agreement with those from commercial PCL filament. In addition to printing photochromic and dual photo- and mechanochromic PCL materials, we also prepare PCL containing a spiropyran unit that is selectively activated by mechanical impetus. Multicomponent specimens containing two different responsive spiropyrans enabled selective activation of different regions within the specimen depending on the stimulus applied to the material. By taking advantage of the unique capabilities of 3D printing, we also demonstrate rapid modification of a prototype force sensor that enables the assessment of peak load by simple visual assessment of mechanochromism. PMID:25478746

  9. 3-D Cavern Enlargement Analyses

    SciTech Connect

    EHGARTNER, BRIAN L.; SOBOLIK, STEVEN R.

    2002-03-01

    Three-dimensional finite element analyses simulate the mechanical response of enlarging existing caverns at the Strategic Petroleum Reserve (SPR). The caverns are located in Gulf Coast salt domes and are enlarged by leaching during oil drawdowns as fresh water is injected to displace the crude oil from the caverns. The current criteria adopted by the SPR limits cavern usage to 5 drawdowns (leaches). As a base case, 5 leaches were modeled over a 25 year period to roughly double the volume of a 19 cavern field. Thirteen additional leaches where then simulated until caverns approached coalescence. The cavern field approximated the geometries and geologic properties found at the West Hackberry site. This enabled comparisons are data collected over nearly 20 years to analysis predictions. The analyses closely predicted the measured surface subsidence and cavern closure rates as inferred from historic well head pressures. This provided the necessary assurance that the model displacements, strains, and stresses are accurate. However, the cavern field has not yet experienced the large scale drawdowns being simulated. Should they occur in the future, code predictions should be validated with actual field behavior at that time. The simulations were performed using JAS3D, a three dimensional finite element analysis code for nonlinear quasi-static solids. The results examine the impacts of leaching and cavern workovers, where internal cavern pressures are reduced, on surface subsidence, well integrity, and cavern stability. The results suggest that the current limit of 5 oil drawdowns may be extended with some mitigative action required on the wells and later on to surface structure due to subsidence strains. The predicted stress state in the salt shows damage to start occurring after 15 drawdowns with significant failure occurring at the 16th drawdown, well beyond the current limit of 5 drawdowns.

  10. Imaging a Sustainable Future in 3D

    NASA Astrophysics Data System (ADS)

    Schuhr, W.; Lee, J. D.; Kanngieser, E.

    2012-07-01

    It is the intention of this paper, to contribute to a sustainable future by providing objective object information based on 3D photography as well as promoting 3D photography not only for scientists, but also for amateurs. Due to the presentation of this article by CIPA Task Group 3 on "3D Photographs in Cultural Heritage", the presented samples are masterpieces of historic as well as of current 3D photography concentrating on cultural heritage. In addition to a report on exemplarily access to international archives of 3D photographs, samples for new 3D photographs taken with modern 3D cameras, as well as by means of a ground based high resolution XLITE staff camera and also 3D photographs taken from a captive balloon and the use of civil drone platforms are dealt with. To advise on optimum suited 3D methodology, as well as to catch new trends in 3D, an updated synoptic overview of the 3D visualization technology, even claiming completeness, has been carried out as a result of a systematic survey. In this respect, e.g., today's lasered crystals might be "early bird" products in 3D, which, due to lack in resolution, contrast and color, remember to the stage of the invention of photography.

  11. Teaching Geography with 3-D Visualization Technology

    ERIC Educational Resources Information Center

    Anthamatten, Peter; Ziegler, Susy S.

    2006-01-01

    Technology that helps students view images in three dimensions (3-D) can support a broad range of learning styles. "Geo-Wall systems" are visualization tools that allow scientists, teachers, and students to project stereographic images and view them in 3-D. We developed and presented 3-D visualization exercises in several undergraduate courses.…

  12. 3D Printing and Its Urologic Applications

    PubMed Central

    Soliman, Youssef; Feibus, Allison H; Baum, Neil

    2015-01-01

    3D printing is the development of 3D objects via an additive process in which successive layers of material are applied under computer control. This article discusses 3D printing, with an emphasis on its historical context and its potential use in the field of urology. PMID:26028997

  13. 3D Flow Visualization Using Texture Advection

    NASA Technical Reports Server (NTRS)

    Kao, David; Zhang, Bing; Kim, Kwansik; Pang, Alex; Moran, Pat (Technical Monitor)

    2001-01-01

    Texture advection is an effective tool for animating and investigating 2D flows. In this paper, we discuss how this technique can be extended to 3D flows. In particular, we examine the use of 3D and 4D textures on 3D synthetic and computational fluid dynamics flow fields.

  14. 3D Elastic Seismic Wave Propagation Code

    1998-09-23

    E3D is capable of simulating seismic wave propagation in a 3D heterogeneous earth. Seismic waves are initiated by earthquake, explosive, and/or other sources. These waves propagate through a 3D geologic model, and are simulated as synthetic seismograms or other graphical output.

  15. 3D Printing and Its Urologic Applications.

    PubMed

    Soliman, Youssef; Feibus, Allison H; Baum, Neil

    2015-01-01

    3D printing is the development of 3D objects via an additive process in which successive layers of material are applied under computer control. This article discusses 3D printing, with an emphasis on its historical context and its potential use in the field of urology. PMID:26028997

  16. The modes of instability of the Antalya (SW-Turkey) coastal cliffs

    NASA Astrophysics Data System (ADS)

    Dipova, Nihat

    2010-05-01

    Cliff erosion and the consequent instabilities present a significant risk to Antalya city (SW-Turkey). Erosional processes include the chemical action of mixing zone water, the mechanical action of waves, salt erosion and biological degradation. The rock properties (lithology, stratification, strength etc) are the controlling factors to this erosion. The coastal cliffs of Antalya are composed of tufa type carbonate rocks which occur in a wide range, from collapsible soil to hard rock. The instability problems of the cliffs of Antalya tufa commonly involve: rock fall, cave collapse, raveling, washout of weakly lithified tufa, shear failure and secondary toppling. Secondary toppling type instabilities, Culmann type failures and complex failures, a combination of these two, are widespread on the cliffs. The occurrence of large failures are usually associated with heavy rainfall as heavy rainfall causes the saturation of pores, increases pore water pressures and reduces the strength of the rock. Comparison between aerial photographs and topographic maps of different dates, do not provide evidence of considerable retreat. According to the historical data there has been little or no cliff retreat for 2000 years. Therefore the erosion rate of the Antalya tufa cliffs is said to be so very slow that retreat is valid only in a geological timescale. However in an engineering timescale these cliffs are subjected to instabilities and to local failures causing local retreats. Keywords: Antalya, Coastal cliff, Cliff erosion, Cliff instability, Tufa

  17. Aerial Photogrammetric Analysis of a Scree Slope and Cliff

    NASA Astrophysics Data System (ADS)

    Saunders, Greg; Galland, Olivier; Mair, Karen

    2014-05-01

    Mapping the physical features of landslide tracks provides information about factors controlling landslide movement. The increasing availability of unmanned aerial vehicles (UAV) provides the opportunity to efficiently and cost effectively map terrain. The main goal of this field study is to create a streamlined work-flow from acquisition to interpretation for the photogrammetric analysis of landslide tracks. Here an open source software package MicMac is used for ortho-image and point-cloud creation. A series of two flights were conducted over a scree (rockfall) slope in Kolsas, Norway. The slope runs roughly 500 m north-south with a maximum width of 60 m. A cliff to the west is the source area for the scree. The cliff consists of conglomerate, basalt, and porphyry from bottom to top respectively. The grain size of boulders in the scree slope apparently varies due to lateral differences in the cliff composition. The flights were completed under cloud cover and consisted of multiple lengthwise passes over the scree field. There was a minimum of 75% overlap between images. During the first flight the altitude was roughly 100 m, the camera was positioned normal to the scree (60 degrees from horizontal), and the resolution was 2.7 cm per pixel. The second flight had an altitude of 200 m, the camera orientation was 30 degrees from horizontal, and the resolution was 4.0 cm per pixel. Using the Micmac engine, Ortho-photos and Digital Elevation Models (DEM) were created for both the scree and the cliff. This data will allow for analysis of grain-size, surface roughness, grain-shape, fracture plane orientation, as well as geological mapping. Further work will focus the quantitative assessment of the significance different camera altitudes and angles have on the results. The work-flow used in this study provides a repeatable method for aerial photogrammetric surveys of scree slopes.

  18. 30. Photocopy of photograph (Frank O. Braynard Collection, Sea Cliff, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. Photocopy of photograph (Frank O. Braynard Collection, Sea Cliff, NY), date and photographer unknown, probably c1935-40 VIEW NORTHWEST OF WEST 55TH ST. PIER INSHORE FACADE; QUEEN OF BERMUDA AND MONARCH OF BERMUDA MOORED ON EITHER SIDE. - West 55th Street & West 56th Street Piers, Hudson River at West Fifty-fifth & West Fifty-sixth Streets, Manhattan, New York County, NY

  19. Rock billboards on the basaltic cliff along the Route 66 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Rock billboards on the basaltic cliff along the Route 66 alignment, ca. 1926. The sign on the left is for "La Bajada Service Shop" (faint "da" visible), "Santa Fe Camp" in the center, and a petroglyph at far right. View facing northwest. - La Bajada Historic Trails and Roads, Approximately 1 mile East/Northeast of intersection of State Highway 16 and Indian Service Road 841, La Bajada, Santa Fe County, NM

  20. Discovery of 4-(dihydropyridinon-3-yl)amino-5-methylthieno[2,3-d]pyrimidine derivatives as potent Mnk inhibitors: synthesis, structure-activity relationship analysis and biological evaluation.

    PubMed

    Yu, Mingfeng; Li, Peng; Basnet, Sunita K C; Kumarasiri, Malika; Diab, Sarah; Teo, Theodosia; Albrecht, Hugo; Wang, Shudong

    2015-05-01

    Phosphorylation of the eukaryotic initiation factor 4E (eIF4E) by mitogen-activated protein kinase (MAPK)-interacting kinases (Mnks) is essential for oncogenesis but unnecessary for normal development. Thus, pharmacological inhibition of Mnks may offer an effective and non-toxic anti-cancer therapeutic strategy. Herein, we report the discovery of 4-(dihydropyridinon-3-yl)amino-5-methylthieno[2,3-d]pyrimidine derivatives as potent Mnk inhibitors. Docking study of 7a in Mnk2 suggests that the compound is stabilised in the ATP binding site through multiple hydrogen bonds and hydrophobic interaction. Cellular mechanistic studies on MV-4-11 cells with leads 7a, 8e and 8f reveal that they are able to down-regulate the phosphorylated eIF4E, Mcl-1 and cyclin D1, and induce apoptosis. PMID:25800647

  1. One-pot solvothermal synthesis of three-dimensional (3D) BiOI/BiOCl composites with enhanced visible-light photocatalytic activities for the degradation of bisphenol-A.

    PubMed

    Xiao, Xin; Hao, Rong; Liang, Min; Zuo, Xiaoxi; Nan, Junmin; Li, Laisheng; Zhang, Weide

    2012-09-30

    Three-dimensional (3D) BiOI/BiOCl composite microspheres with enhanced visible-light photodegradation activity of bisphenol-A (BPA) are synthesized by a simple, one-pot, template-free, solvothermal method using BiI(3) and BiCl(3) as precursors. These 3D hierarchical microspheres with heterojunction structures are composed of 2D nanosheets and have composition-dependent absorption properties in the ultraviolet and visible light regions. The photocatalytic oxidation of BPA over BiOI/BiOCl composites followed pseudo first-order kinetics according to the Langmuir-Hinshelwood model. The highest photodegradation efficiency of BPA, i.e., nearly 100%, was observed with the BiOI/BiOCl composite (containing 90% BiOI) using a catalyst dosage of 1 g L(-1) in the BPA solution (C(0)=20 mg L(-1), pH=7.0) under visible light irradiation for 60 min. Under these conditions, the reaction rate constant was more than 4 and 20 times greater than that of pure BiOI and the commercially available Degussa P25, respectively. The superior photocatalytic activity of this composite catalyst is attributed to the suitable band gap energies and the low recombination rate of the photogenerated electron-hole pairs due to the presence of BiOI/BiOCl heterostructures. Only one intermediate at m/z 151 was observed in the photodegradation process of BPA by liquid chromatography combined with mass spectrometry (LC-MS) analysis, and a simple and hole-predominated photodegradation pathway of BPA was subsequently proposed. Furthermore, this photocatalyst exhibited a high mineralization ratio, high stability and easy separation for recycling use, suggesting that it is a promising photocatalyst for the removal of BPA pollutants. PMID:22818177

  2. 3-D Perspective Pasadena, California

    NASA Technical Reports Server (NTRS)

    2000-01-01

    This perspective view shows the western part of the city of Pasadena, California, looking north towards the San Gabriel Mountains. Portions of the cities of Altadena and La Canada, Flintridge are also shown. The image was created from three datasets: the Shuttle Radar Topography Mission (SRTM) supplied the elevation data; Landsat data from November 11, 1986 provided the land surface color (not the sky) and U.S. Geological Survey digital aerial photography provides the image detail. The Rose Bowl, surrounded by a golf course, is the circular feature at the bottom center of the image. The Jet Propulsion Laboratory is the cluster of large buildings north of the Rose Bowl at the base of the mountains. A large landfill, Scholl Canyon, is the smooth area in the lower left corner of the scene. This image shows the power of combining data from different sources to create planning tools to study problems that affect large urban areas. In addition to the well-known earthquake hazards, Southern California is affected by a natural cycle of fire and mudflows. Wildfires strip the mountains of vegetation, increasing the hazards from flooding and mudflows for several years afterwards. Data such as shown on this image can be used to predict both how wildfires will spread over the terrain and also how mudflows will be channeled down the canyons. The Shuttle Radar Topography Mission (SRTM), launched on February 11, 2000, uses the same radar instrument that comprised the Spaceborne Imaging Radar-C/X-Band Synthetic Aperture Radar (SIR-C/X-SAR) that flew twice on the Space Shuttle Endeavour in 1994. The mission was designed to collect three dimensional measurements of the Earth's surface. To collect the 3-D data, engineers added a 60-meter-long (200-foot) mast, an additional C-band imaging antenna and improved tracking and navigation devices. The mission is a cooperative project between the National Aeronautics and Space Administration (NASA), the National Imagery and Mapping Agency

  3. The Esri 3D city information model

    NASA Astrophysics Data System (ADS)

    Reitz, T.; Schubiger-Banz, S.

    2014-02-01

    With residential and commercial space becoming increasingly scarce, cities are going vertical. Managing the urban environments in 3D is an increasingly important and complex undertaking. To help solving this problem, Esri has released the ArcGIS for 3D Cities solution. The ArcGIS for 3D Cities solution provides the information model, tools and apps for creating, analyzing and maintaining a 3D city using the ArcGIS platform. This paper presents an overview of the 3D City Information Model and some sample use cases.

  4. Case study: Beauty and the Beast 3D: benefits of 3D viewing for 2D to 3D conversion

    NASA Astrophysics Data System (ADS)

    Handy Turner, Tara

    2010-02-01

    From the earliest stages of the Beauty and the Beast 3D conversion project, the advantages of accurate desk-side 3D viewing was evident. While designing and testing the 2D to 3D conversion process, the engineering team at Walt Disney Animation Studios proposed a 3D viewing configuration that not only allowed artists to "compose" stereoscopic 3D but also improved efficiency by allowing artists to instantly detect which image features were essential to the stereoscopic appeal of a shot and which features had minimal or even negative impact. At a time when few commercial 3D monitors were available and few software packages provided 3D desk-side output, the team designed their own prototype devices and collaborated with vendors to create a "3D composing" workstation. This paper outlines the display technologies explored, final choices made for Beauty and the Beast 3D, wish-lists for future development and a few rules of thumb for composing compelling 2D to 3D conversions.

  5. Printing 3D dielectric elastomer actuators for soft robotics

    NASA Astrophysics Data System (ADS)

    Rossiter, Jonathan; Walters, Peter; Stoimenov, Boyko

    2009-03-01

    We present a new approach to the fabrication of soft dielectric elastomer actuators using a 3D printing process. Complete actuators including active membranes and support structures can be 3D printed in one go, resulting in a great improvement in fabrication speed and increases in accuracy and consistency. We describe the fabrication process and present force and displacement results for a double-membrane antagonistic actuator. In this structure controlled prestrain is applied by the simple process of pressing together two printed actuator halves. The development of 3D printable soft actuators will have a large impact on many application areas including engineering, medicine and the emerging field of soft robotics.

  6. 3D printing of microscopic bacterial communities

    PubMed Central

    Connell, Jodi L.; Ritschdorff, Eric T.; Whiteley, Marvin; Shear, Jason B.

    2013-01-01

    Bacteria communicate via short-range physical and chemical signals, interactions known to mediate quorum sensing, sporulation, and other adaptive phenotypes. Although most in vitro studies examine bacterial properties averaged over large populations, the levels of key molecular determinants of bacterial fitness and pathogenicity (e.g., oxygen, quorum-sensing signals) may vary over micrometer scales within small, dense cellular aggregates believed to play key roles in disease transmission. A detailed understanding of how cell–cell interactions contribute to pathogenicity in natural, complex environments will require a new level of control in constructing more relevant cellular models for assessing bacterial phenotypes. Here, we describe a microscopic three-dimensional (3D) printing strategy that enables multiple populations of bacteria to be organized within essentially any 3D geometry, including adjacent, nested, and free-floating colonies. In this laser-based lithographic technique, microscopic containers are formed around selected bacteria suspended in gelatin via focal cross-linking of polypeptide molecules. After excess reagent is removed, trapped bacteria are localized within sealed cavities formed by the cross-linked gelatin, a highly porous material that supports rapid growth of fully enclosed cellular populations and readily transmits numerous biologically active species, including polypeptides, antibiotics, and quorum-sensing signals. Using this approach, we show that a picoliter-volume aggregate of Staphylococcus aureus can display substantial resistance to β-lactam antibiotics by enclosure within a shell composed of Pseudomonas aeruginosa. PMID:24101503

  7. Investigating a rock instability. Exemple of the "Bornes du Diable" cliff, Switzerland

    NASA Astrophysics Data System (ADS)

    Franz, Martin; Michoud, Clément; Güell Pons, Maria; Pedrazzini, Andrea; Jaboyedoff, Michel

    2010-05-01

    The « Bornes du Diable » cliff is located in the Val de Bagnes in south western Valais. In September 2008, a block of about 2000 m3 has fallen down causing damages to a small alpine road. This event shows the potential instability of the whole cliff which represents a larger volume. Indeed it measures 160 m high and about 100 m width. Several back-cracks are visible hundred meters back to the actual crest. The road, the reservoir of Mauvoisin and the related dam could be reached by the rockfalls. The goal of this study is to characterize the structural settings and to quantify the previous displacements that occurred in the area in order to assess the present day stability. Structural, geomorphological and geomechanical characterization have been carried by field investigations and completed by orthophotos and digital elevation model analysis. The unstable area was scanned in fall 2009 using terrestrial laser scanner (TLS) in order to have a very detailed topography and to perform a structural analysis using the home made software Coltop3D. Differential GPS campaign was initiated in July 2009 and will be continued for the next years to assess the potential movement affecting the entire area. Moreover, a photogrammetric analysis of two aerial photographs of 1979 and 2000 is performed to identify if previous movements occurred in the area. The potential unstable volume is estimated performing the Sloping Local Base Level method and geometrical analysis. Finally a model is performed with the UDEC™ and FLAC® softwares. The movement, the discontinuity sets, the rockfall mechanisms and the triggering factors are analyzed to define the deep structures and the mechanisms of a potential complete collapse. The preliminary results indicate an important rock fracturing in the area of the instability. The persistence schistosity and the highly weathered rock surface indicating a fair-poor rock mass quality. TLS point cloud analysis using Coltop3D highlights six

  8. 3D laptop for defense applications

    NASA Astrophysics Data System (ADS)

    Edmondson, Richard; Chenault, David

    2012-06-01

    Polaris Sensor Technologies has developed numerous 3D display systems using a US Army patented approach. These displays have been developed as prototypes for handheld controllers for robotic systems and closed hatch driving, and as part of a TALON robot upgrade for 3D vision, providing depth perception for the operator for improved manipulation and hazard avoidance. In this paper we discuss the prototype rugged 3D laptop computer and its applications to defense missions. The prototype 3D laptop combines full temporal and spatial resolution display with the rugged Amrel laptop computer. The display is viewed through protective passive polarized eyewear, and allows combined 2D and 3D content. Uses include robot tele-operation with live 3D video or synthetically rendered scenery, mission planning and rehearsal, enhanced 3D data interpretation, and simulation.

  9. A High-Throughput Screen Identifies 2,9-Diazaspiro[5.5]Undecanes as Inducers of the Endoplasmic Reticulum Stress Response with Cytotoxic Activity in 3D Glioma Cell Models

    PubMed Central

    Yasgar, Adam; Lea, Wendy A.; Sun, Hongmao; Wang, Yuhong; Luci, Diane K.; Yang, Shyh-Ming; Nishihara, Kana; Takeda, Shunichi; Sagor, Mohiuddin; Earnshaw, Irina; Okada, Tetsuya; Mori, Kazutoshi; Wilson, Kelli; Riggins, Gregory J.; Xia, Menghang; Grimaldi, Maurizio; Jadhav, Ajit; Maloney, David J.; Simeonov, Anton

    2016-01-01

    The endoplasmic reticulum (ER) is involved in Ca2+ signaling and protein folding. ER Ca2+ depletion and accumulation of unfolded proteins activate the molecular chaperone GRP78 (glucose-regulated protein 78) which in turn triggers the ER stress response (ERSR) pathway aimed to restore ER homeostasis. Failure to adapt to stress, however, results in apoptosis. We and others have shown that malignant cells are more susceptible to ERSR-induced apoptosis than their normal counterparts, implicating the ERSR as a potential target for cancer therapeutics. Predicated on these findings, we developed an assay that uses a GRP78 biosensor to identify small molecule activators of ERSR in glioma cells. We performed a quantitative high-throughput screen (qHTS) against a collection of ~425,000 compounds and a comprehensive panel of orthogonal secondary assays was formulated for stringent compound validation. We identified novel activators of ERSR, including a compound with a 2,9-diazaspiro[5.5]undecane core, which depletes intracellular Ca2+ stores and induces apoptosis-mediated cell death in several cancer cell lines, including patient-derived and 3D cultures of glioma cells. This study demonstrates that our screening platform enables the identification and profiling of ERSR inducers with cytotoxic activity and advocates for characterization of these compound in in vivo models. PMID:27570969

  10. Filling gaps in cultural heritage documentation by 3D photography