Science.gov

Sample records for 3d multiplexer integrated

  1. 3D multiplexed immunoplasmonics microscopy

    NASA Astrophysics Data System (ADS)

    Bergeron, Éric; Patskovsky, Sergiy; Rioux, David; Meunier, Michel

    2016-07-01

    Selective labelling, identification and spatial distribution of cell surface biomarkers can provide important clinical information, such as distinction between healthy and diseased cells, evolution of a disease and selection of the optimal patient-specific treatment. Immunofluorescence is the gold standard for efficient detection of biomarkers expressed by cells. However, antibodies (Abs) conjugated to fluorescent dyes remain limited by their photobleaching, high sensitivity to the environment, low light intensity, and wide absorption and emission spectra. Immunoplasmonics is a novel microscopy method based on the visualization of Abs-functionalized plasmonic nanoparticles (fNPs) targeting cell surface biomarkers. Tunable fNPs should provide higher multiplexing capacity than immunofluorescence since NPs are photostable over time, strongly scatter light at their plasmon peak wavelengths and can be easily functionalized. In this article, we experimentally demonstrate accurate multiplexed detection based on the immunoplasmonics approach. First, we achieve the selective labelling of three targeted cell surface biomarkers (cluster of differentiation 44 (CD44), epidermal growth factor receptor (EGFR) and voltage-gated K+ channel subunit KV1.1) on human cancer CD44+ EGFR+ KV1.1+ MDA-MB-231 cells and reference CD44- EGFR- KV1.1+ 661W cells. The labelling efficiency with three stable specific immunoplasmonics labels (functionalized silver nanospheres (CD44-AgNSs), gold (Au) NSs (EGFR-AuNSs) and Au nanorods (KV1.1-AuNRs)) detected by reflected light microscopy (RLM) is similar to the one with immunofluorescence. Second, we introduce an improved method for 3D localization and spectral identification of fNPs based on fast z-scanning by RLM with three spectral filters corresponding to the plasmon peak wavelengths of the immunoplasmonics labels in the cellular environment (500 nm for 80 nm AgNSs, 580 nm for 100 nm AuNSs and 700 nm for 40 nm × 92 nm AuNRs). Third, the developed

  2. Free-space coherent optical communication with orbital angular, momentum multiplexing/demultiplexing using a hybrid 3D photonic integrated circuit.

    PubMed

    Guan, Binbin; Scott, Ryan P; Qin, Chuan; Fontaine, Nicolas K; Su, Tiehui; Ferrari, Carlo; Cappuzzo, Mark; Klemens, Fred; Keller, Bob; Earnshaw, Mark; Yoo, S J B

    2014-01-13

    We demonstrate free-space space-division-multiplexing (SDM) with 15 orbital angular momentum (OAM) states using a three-dimensional (3D) photonic integrated circuit (PIC). The hybrid device consists of a silica planar lightwave circuit (PLC) coupled to a 3D waveguide circuit to multiplex/demultiplex OAM states. The low excess loss hybrid device is used in individual and two simultaneous OAM states multiplexing and demultiplexing link experiments with a 20 Gb/s, 1.67 b/s/Hz quadrature phase shift keyed (QPSK) signal, which shows error-free performance for 379,960 tested bits for all OAM states.

  3. 3D origami-based multifunction-integrated immunodevice: low-cost and multiplexed sandwich chemiluminescence immunoassay on microfluidic paper-based analytical device.

    PubMed

    Ge, Lei; Wang, Shoumei; Song, Xianrang; Ge, Shenguang; Yu, Jinghua

    2012-09-07

    A novel 3D microfluidic paper-based immunodevice, integrated with blood plasma separation from whole blood samples, automation of rinse steps, and multiplexed CL detections, was developed for the first time based on the principle of origami (denoted as origami-based device). This 3D origami-based device, comprised of one test pad surrounded by four folding tabs, could be patterned and fabricated by wax-printing on paper in bulk. In this work, a sandwich-type chemiluminescence (CL) immunoassay was introduced into this 3D origami-based immunodevice, which could separate the operational procedures into several steps including (i) folding pads above/below and (ii) addition of reagent/buffer under a specific sequence. The CL behavior, blood plasma separation, washing protocol, and incubation time were investigated in this work. The developed 3D origami-based CL immunodevice, combined with a typical luminuol-H(2)O(2) CL system and catalyzed by Ag nanoparticles, showed excellent analytical performance for the simultaneous detection of four tumor markers. The whole blood samples were assayed and the results obtained were in agreement with the reference values from the parallel single-analyte test. This paper-based microfluidic origami CL detection system provides a new strategy for a low-cost, sensitive, simultaneous multiplex immunoassay and point-of-care diagnostics.

  4. High density 3D printed microfluidic valves, pumps, and multiplexers.

    PubMed

    Gong, Hua; Woolley, Adam T; Nordin, Gregory P

    2016-07-07

    In this paper we demonstrate that 3D printing with a digital light processor stereolithographic (DLP-SLA) 3D printer can be used to create high density microfluidic devices with active components such as valves and pumps. Leveraging our previous work on optical formulation of inexpensive resins (RSC Adv., 2015, 5, 106621), we demonstrate valves with only 10% of the volume of our original 3D printed valves (Biomicrofluidics, 2015, 9, 016501), which were already the smallest that have been reported. Moreover, we show that incorporation of a thermal initiator in the resin formulation along with a post-print bake can dramatically improve the durability of 3D printed valves up to 1 million actuations. Using two valves and a valve-like displacement chamber (DC), we also create compact 3D printed pumps. With 5-phase actuation and a 15 ms phase interval, we obtain pump flow rates as high as 40 μL min(-1). We also characterize maximum pump back pressure (i.e., maximum pressure the pump can work against), maximum flow rate (flow rate when there is zero back pressure), and flow rate as a function of the height of the pump outlet. We further demonstrate combining 5 valves and one DC to create a 3-to-2 multiplexer with integrated pump. In addition to serial multiplexing, we also show that the device can operate as a mixer. Importantly, we illustrate the rapid fabrication and test cycles that 3D printing makes possible by implementing a new multiplexer design to improve mixing, and fabricate and test it within one day.

  5. 3D Scan Systems Integration

    DTIC Science & Technology

    2007-11-02

    AGENCY USE ONLY (Leave Blank) 2. REPORT DATE 5 Feb 98 4. TITLE AND SUBTITLE 3D Scan Systems Integration REPORT TYPE AND DATES COVERED...2-89) Prescribed by ANSI Std. Z39-1 298-102 [ EDO QUALITY W3PECTEDI DLA-ARN Final Report for US Defense Logistics Agency on DDFG-T2/P3: 3D...SCAN SYSTEMS INTEGRATION Contract Number SPO100-95-D-1014 Contractor Ohio University Delivery Order # 0001 Delivery Order Title 3D Scan Systems

  6. Ultrafast laser inscription of 3D components for spatial multiplexing

    NASA Astrophysics Data System (ADS)

    Thomson, Robert R.

    2016-02-01

    The thirst for bandwidth in telecommunications networks is becoming ever larger due to bandwidth hungry applications such as video-on-demand. To further increase the bandwidth capacity, engineers are now seeking to imprint information on the last remaining degree of freedom of the lightwave carrier - space. This has given rise to the field of Space Division Multiplexing (SDM). In essence, the concept of SDM simple; we aim to use the different spatial modes of an optical fibre as multiplexed data transmission channels. These modes could either be in the form of separate singlemodes in a multicore optical fibre, individual spatial modes of a multimode fibre, or indeed the individual spatial modes of a multimode multicore optical fibre. Regardless of the particular "flavour" of SDM in question, it is clear that significant interfacing issues exist between the optical fibres used in SDM and the conventional single-mode planar lightwave circuits that are essential to process the light (e.g. arrayed waveguide gratings and splitters), and efficient interconnect technologies will be required. One fabrication technology that has emerged as a possible route to solve these interconnection issues is ultrafast laser inscription (ULI), which relies on the use of focused ultrashort laser pulses to directly inscribe three-dimensional waveguide structures inside a bulk dielectric. In this paper, I describe some of the work that has been conducted around the world to apply the unique waveguide fabrication capabilities of ULI to the development of 3D photonic components for applications in SDM.

  7. Field lens multiplexing in holographic 3D displays by using Bragg diffraction based volume gratings

    NASA Astrophysics Data System (ADS)

    Fütterer, G.

    2016-11-01

    Applications, which can profit from holographic 3D displays, are the visualization of 3D data, computer-integrated manufacturing, 3D teleconferencing and mobile infotainment. However, one problem of holographic 3D displays, which are e.g. based on space bandwidth limited reconstruction of wave segments, is to realize a small form factor. Another problem is to provide a reasonable large volume for the user placement, which means to provide an acceptable freedom of movement. Both problems should be solved without decreasing the image quality of virtual and real object points, which are generated within the 3D display volume. A diffractive optical design using thick hologram gratings, which can be referred to as Bragg diffraction based volume gratings, can provide a small form factor and high definition natural viewing experience of 3D objects. A large collimated wave can be provided by an anamorphic backlight unit. The complex valued spatial light modulator add local curvatures to the wave field he is illuminated with. The modulated wave field is focused onto to the user plane by using a volume grating based field lens. Active type liquid crystal gratings provide 1D fine tracking of approximately +/- 8° deg. Diffractive multiplex has to be implemented for each color and for a set of focus functions providing coarse tracking. Boundary conditions of the diffractive multiplexing are explained. This is done in regards to the display layout and by using the coupled wave theory (CWT). Aspects of diffractive cross talk and its suppression will be discussed including longitudinal apodized volume gratings.

  8. 3D packaging for integrated circuit systems

    SciTech Connect

    Chu, D.; Palmer, D.W.

    1996-11-01

    A goal was set for high density, high performance microelectronics pursued through a dense 3D packing of integrated circuits. A {open_quotes}tool set{close_quotes} of assembly processes have been developed that enable 3D system designs: 3D thermal analysis, silicon electrical through vias, IC thinning, mounting wells in silicon, adhesives for silicon stacking, pretesting of IC chips before commitment to stacks, and bond pad bumping. Validation of these process developments occurred through both Sandia prototypes and subsequent commercial examples.

  9. 3D integral imaging with optical processing

    NASA Astrophysics Data System (ADS)

    Martínez-Corral, Manuel; Martínez-Cuenca, Raúl; Saavedra, Genaro; Javidi, Bahram

    2008-04-01

    Integral imaging (InI) systems are imaging devices that provide auto-stereoscopic images of 3D intensity objects. Since the birth of this new technology, InI systems have faced satisfactorily many of their initial drawbacks. Basically, two kind of procedures have been used: digital and optical procedures. The "3D Imaging and Display Group" at the University of Valencia, with the essential collaboration of Prof. Javidi, has centered its efforts in the 3D InI with optical processing. Among other achievements, our Group has proposed the annular amplitude modulation for enlargement of the depth of field, dynamic focusing for reduction of the facet-braiding effect, or the TRES and MATRES devices to enlarge the viewing angle.

  10. Self assembled structures for 3D integration

    NASA Astrophysics Data System (ADS)

    Rao, Madhav

    Three dimensional (3D) micro-scale structures attached to a silicon substrate have various applications in microelectronics. However, formation of 3D structures using conventional micro-fabrication techniques are not efficient and require precise control of processing parameters. Self assembly is a method for creating 3D structures that takes advantage of surface area minimization phenomena. Solder based self assembly (SBSA), the subject of this dissertation, uses solder as a facilitator in the formation of 3D structures from 2D patterns. Etching a sacrificial layer underneath a portion of the 2D pattern allows the solder reflow step to pull those areas out of the substrate plane resulting in a folded 3D structure. Initial studies using the SBSA method demonstrated low yields in the formation of five different polyhedra. The failures in folding were primarily attributed to nonuniform solder deposition on the underlying metal pads. The dip soldering method was analyzed and subsequently refined. A modified dip soldering process provided improved yield among the polyhedra. Solder bridging referred as joining of solder deposited on different metal patterns in an entity influenced the folding mechanism. In general, design parameters such as small gap-spacings and thick metal pads were found to favor solder bridging for all patterns studied. Two types of soldering: face and edge soldering were analyzed. Face soldering refers to the application of solder on the entire metal face. Edge soldering indicates application of solder only on the edges of the metal face. Mechanical grinding showed that face soldered SBSA structures were void free and robust in nature. In addition, the face soldered 3D structures provide a consistent heat resistant solder standoff height that serve as attachments in the integration of dissimilar electronic technologies. Face soldered 3D structures were developed on the underlying conducting channel to determine the thermo-electric reliability of

  11. 3D Integration for Wireless Multimedia

    NASA Astrophysics Data System (ADS)

    Kimmich, Georg

    The convergence of mobile phone, internet, mapping, gaming and office automation tools with high quality video and still imaging capture capability is becoming a strong market trend for portable devices. High-density video encode and decode, 3D graphics for gaming, increased application-software complexity and ultra-high-bandwidth 4G modem technologies are driving the CPU performance and memory bandwidth requirements close to the PC segment. These portable multimedia devices are battery operated, which requires the deployment of new low-power-optimized silicon process technologies and ultra-low-power design techniques at system, architecture and device level. Mobile devices also need to comply with stringent silicon-area and package-volume constraints. As for all consumer devices, low production cost and fast time-to-volume production is key for success. This chapter shows how 3D architectures can bring a possible breakthrough to meet the conflicting power, performance and area constraints. Multiple 3D die-stacking partitioning strategies are described and analyzed on their potential to improve the overall system power, performance and cost for specific application scenarios. Requirements and maturity of the basic process-technology bricks including through-silicon via (TSV) and die-to-die attachment techniques are reviewed. Finally, we highlight new challenges which will arise with 3D stacking and an outlook on how they may be addressed: Higher power density will require thermal design considerations, new EDA tools will need to be developed to cope with the integration of heterogeneous technologies and to guarantee signal and power integrity across the die stack. The silicon/wafer test strategies have to be adapted to handle high-density IO arrays, ultra-thin wafers and provide built-in self-test of attached memories. New standards and business models have to be developed to allow cost-efficient assembly and testing of devices from different silicon and technology

  12. Integration of real-time 3D image acquisition and multiview 3D display

    NASA Astrophysics Data System (ADS)

    Zhang, Zhaoxing; Geng, Zheng; Li, Tuotuo; Li, Wei; Wang, Jingyi; Liu, Yongchun

    2014-03-01

    Seamless integration of 3D acquisition and 3D display systems offers enhanced experience in 3D visualization of the real world objects or scenes. The vivid representation of captured 3D objects displayed on a glasses-free 3D display screen could bring the realistic viewing experience to viewers as if they are viewing real-world scene. Although the technologies in 3D acquisition and 3D display have advanced rapidly in recent years, effort is lacking in studying the seamless integration of these two different aspects of 3D technologies. In this paper, we describe our recent progress on integrating a light-field 3D acquisition system and an autostereoscopic multiview 3D display for real-time light field capture and display. This paper focuses on both the architecture design and the implementation of the hardware and the software of this integrated 3D system. A prototype of the integrated 3D system is built to demonstrate the real-time 3D acquisition and 3D display capability of our proposed system.

  13. Integrated multiplexed capillary electrophoresis system

    DOEpatents

    Yeung, Edward S.; Tan, Hongdong

    2002-05-14

    The present invention provides an integrated multiplexed capillary electrophoresis system for the analysis of sample analytes. The system integrates and automates multiple components, such as chromatographic columns and separation capillaries, and further provides a detector for the detection of analytes eluting from the separation capillaries. The system employs multiplexed freeze/thaw valves to manage fluid flow and sample movement. The system is computer controlled and is capable of processing samples through reaction, purification, denaturation, pre-concentration, injection, separation and detection in parallel fashion. Methods employing the system of the invention are also provided.

  14. Multiplexing encoding method for full-color dynamic 3D holographic display.

    PubMed

    Xue, Gaolei; Liu, Juan; Li, Xin; Jia, Jia; Zhang, Zhao; Hu, Bin; Wang, Yongtian

    2014-07-28

    The multiplexing encoding method is proposed and demonstrated for reconstructing colorful images accurately by using single phase-only spatial light modulator (SLM). It will encode the light waves at different wavelengths into one pure-phase hologram at the same time based on the analytic formulas. The three-dimensional (3D) images can be reconstructed clearly when the light waves at different wavelengths are incident into the encoding hologram. Numerical simulations and optical experiments for 2D and 3D colorful images are performed. The results show that the colorful reconstructed images with high quality are achieved successfully. The proposed multiplexing method is a simple and fast encoding approach and the size of the system is small and compact. It is expected to be used for realizing full-color 3D holographic display in future.

  15. A novel time-multiplexed autostereoscopic multiview full resolution 3D display

    NASA Astrophysics Data System (ADS)

    Liou, Jian-Chiun; Chen, Fu-Hao

    2012-03-01

    Many people believe that in the future, autostereoscopic 3D displays will become a mainstream display type. Achievement of higher quality 3D images requires both higher panel resolution and more viewing zones. Consequently, the transmission bandwidth of the 3D display systems involves enormous amounts of data transfer. We propose and experimentally demonstrate a novel time-multiplexed autostereoscopic multi-view full resolution 3D display based on the lenticular lens array in association with the control of the active dynamic LED backlight. The lenticular lenses of the lens array optical system receive the light and deflect the light into each viewing zone in a time sequence. The crosstalk under different observation scanning angles is showed, including the cases of 4-views field scanning. The crosstalk of any view zones is about 5% respectively; the results are better than other 3D type.

  16. Hardware Trust Implications of 3-D Integration

    DTIC Science & Technology

    2010-12-01

    enhancing a commod- ity processor with a variety of security functions. This paper examines the 3-D design approach and provides an analysis concluding...of key components. The question addressed by this paper is, “Can a 3-D control plane provide useful secure services when it is conjoined with an...untrust- worthy computation plane?” Design-level investigation of this question yields a definite yes. This paper explores 3- D applications and their

  17. Development of 3D integrated circuits for HEP

    SciTech Connect

    Yarema, R.; /Fermilab

    2006-09-01

    Three dimensional integrated circuits are well suited to improving circuit bandwidth and increasing effective circuit density. Recent advances in industry have made 3D integrated circuits an option for HEP. The 3D technology is discussed in this paper and several examples are shown. Design of a 3D demonstrator chip for the ILC is presented.

  18. Mesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography.

    PubMed

    Chen, Lingling; Alexandrov, Yuriy; Kumar, Sunil; Andrews, Natalie; Dallman, Margaret J; French, Paul M W; McGinty, James

    2015-04-01

    We describe an angular multiplexed imaging technique for 3-D in vivo cell tracking of sparse cell distributions and optical projection tomography (OPT) with superior time-lapse resolution and a significantly reduced light dose compared to volumetric time-lapse techniques. We demonstrate that using dual axis OPT, where two images are acquired simultaneously at different projection angles, can enable localization and tracking of features in 3-D with a time resolution equal to the camera frame rate. This is achieved with a 200x reduction in light dose compared to an equivalent volumetric time-lapse single camera OPT acquisition with 200 projection angles. We demonstrate the application of this technique to mapping the 3-D neutrophil migration pattern observed over ~25.5 minutes in a live 2 day post-fertilisation transgenic LysC:GFP zebrafish embryo following a tail wound.

  19. Parallel Imaging of 3D Surface Profile with Space-Division Multiplexing

    PubMed Central

    Lee, Hyung Seok; Cho, Soon-Woo; Kim, Gyeong Hun; Jeong, Myung Yung; Won, Young Jae; Kim, Chang-Seok

    2016-01-01

    We have developed a modified optical frequency domain imaging (OFDI) system that performs parallel imaging of three-dimensional (3D) surface profiles by using the space division multiplexing (SDM) method with dual-area swept sourced beams. We have also demonstrated that 3D surface information for two different areas could be well obtained in a same time with only one camera by our method. In this study, double field of views (FOVs) of 11.16 mm × 5.92 mm were achieved within 0.5 s. Height range for each FOV was 460 µm and axial and transverse resolutions were 3.6 and 5.52 µm, respectively. PMID:26805840

  20. Three wafer stacking for 3D integration.

    SciTech Connect

    Greth, K. Douglas; Ford, Christine L.; Lantz, Jeffrey W.; Shinde, Subhash L.; Timon, Robert P.; Bauer, Todd M.; Hetherington, Dale Laird; Sanchez, Carlos Anthony

    2011-11-01

    Vertical wafer stacking will enable a wide variety of new system architectures by enabling the integration of dissimilar technologies in one small form factor package. With this LDRD, we explored the combination of processes and integration techniques required to achieve stacking of three or more layers. The specific topics that we investigated include design and layout of a reticle set for use as a process development vehicle, through silicon via formation, bonding media, wafer thinning, dielectric deposition for via isolation on the wafer backside, and pad formation.

  1. Wafer-Level 3D Integration for ULSI Interconnects

    NASA Astrophysics Data System (ADS)

    Gutmann, Ronald J.; Lu, Jian-Qiang

    Three-dimensional (3D) integration in a system-in-a-package (SiP) implementation (packaging-based 3D) is becoming increasingly used in consumer, computer, and communication applications where form factor is critical. In particular, the hand-held market for a growing myriad of voice, data, messaging, and imaging products is enabled by packaging-based 3D integration (i.e., stacking and connecting individual chips). The key drivers are for increased memory capacity and for heterogeneous integration of different IC technologies and functions.

  2. Multiplexed 3D FRET imaging in deep tissue of live embryos

    PubMed Central

    Zhao, Ming; Wan, Xiaoyang; Li, Yu; Zhou, Weibin; Peng, Leilei

    2015-01-01

    Current deep tissue microscopy techniques are mostly restricted to intensity mapping of fluorophores, which significantly limit their applications in investigating biochemical processes in vivo. We present a deep tissue multiplexed functional imaging method that probes multiple Förster resonant energy transfer (FRET) sensors in live embryos with high spatial resolution. The method simultaneously images fluorescence lifetimes in 3D with multiple excitation lasers. Through quantitative analysis of triple-channel intensity and lifetime images, we demonstrated that Ca2+ and cAMP levels of live embryos expressing dual FRET sensors can be monitored simultaneously at microscopic resolution. The method is compatible with a broad range of FRET sensors currently available for probing various cellular biochemical functions. It opens the door to imaging complex cellular circuitries in whole live organisms. PMID:26387920

  3. 3D augmented reality with integral imaging display

    NASA Astrophysics Data System (ADS)

    Shen, Xin; Hua, Hong; Javidi, Bahram

    2016-06-01

    In this paper, a three-dimensional (3D) integral imaging display for augmented reality is presented. By implementing the pseudoscopic-to-orthoscopic conversion method, elemental image arrays with different capturing parameters can be transferred into the identical format for 3D display. With the proposed merging algorithm, a new set of elemental images for augmented reality display is generated. The newly generated elemental images contain both the virtual objects and real world scene with desired depth information and transparency parameters. The experimental results indicate the feasibility of the proposed 3D augmented reality with integral imaging.

  4. Polarimetric 3D integral imaging in photon-starved conditions.

    PubMed

    Carnicer, Artur; Javidi, Bahram

    2015-03-09

    We develop a method for obtaining 3D polarimetric integral images from elemental images recorded in low light illumination conditions. Since photon-counting images are very sparse, calculation of the Stokes parameters and the degree of polarization should be handled carefully. In our approach, polarimetric 3D integral images are generated using the Maximum Likelihood Estimation and subsequently reconstructed by means of a Total Variation Denoising filter. In this way, polarimetric results are comparable to those obtained in conventional illumination conditions. We also show that polarimetric information retrieved from photon starved images can be used in 3D object recognition problems. To the best of our knowledge, this is the first report on 3D polarimetric photon counting integral imaging.

  5. Progresses in 3D integral imaging with optical processing

    NASA Astrophysics Data System (ADS)

    Martínez-Corral, Manuel; Martínez-Cuenca, Raúl; Saavedra, Genaro; Navarro, Héctor; Pons, Amparo; Javidi, Bahram

    2008-11-01

    Integral imaging is a promising technique for the acquisition and auto-stereoscopic display of 3D scenes with full parallax and without the need of any additional devices like special glasses. First suggested by Lippmann in the beginning of the 20th century, integral imaging is based in the intersection of ray cones emitted by a collection of 2D elemental images which store the 3D information of the scene. This paper is devoted to the study, from the ray optics point of view, of the optical effects and interaction with the observer of integral imaging systems.

  6. Surviving sepsis--a 3D integrative educational simulator.

    PubMed

    Ježek, Filip; Tribula, Martin; Kulhánek, Tomáš; Mateják, Marek; Privitzer, Pavol; Šilar, Jan; Kofránek, Jiří; Lhotská, Lenka

    2015-08-01

    Computer technology offers greater educational possibilities, notably simulation and virtual reality. This paper presents a technology which serves to integrate multiple modalities, namely 3D virtual reality, node-based simulator, Physiomodel explorer and explanatory physiological simulators employing Modelica language and Unity3D platform. This emerging tool chain should allow the authors to concentrate more on educational content instead of application development. The technology is demonstrated through Surviving sepsis educational scenario, targeted on Microsoft Windows Store platform.

  7. Accommodation response measurements for integral 3D image

    NASA Astrophysics Data System (ADS)

    Hiura, H.; Mishina, T.; Arai, J.; Iwadate, Y.

    2014-03-01

    We measured accommodation responses under integral photography (IP), binocular stereoscopic, and real object display conditions, and viewing conditions of binocular and monocular viewing conditions. The equipment we used was an optometric device and a 3D display. We developed the 3D display for IP and binocular stereoscopic images that comprises a high-resolution liquid crystal display (LCD) and a high-density lens array. The LCD has a resolution of 468 dpi and a diagonal size of 4.8 inches. The high-density lens array comprises 106 x 69 micro lenses that have a focal length of 3 mm and diameter of 1 mm. The lenses are arranged in a honeycomb pattern. The 3D display was positioned 60 cm from an observer under IP and binocular stereoscopic display conditions. The target was presented at eight depth positions relative to the 3D display: 15, 10, and 5 cm in front of the 3D display, on the 3D display panel, and 5, 10, 15 and 30 cm behind the 3D display under the IP and binocular stereoscopic display conditions. Under the real object display condition, the target was displayed on the 3D display panel, and the 3D display was placed at the eight positions. The results suggest that the IP image induced more natural accommodation responses compared to the binocular stereoscopic image. The accommodation responses of the IP image were weaker than those of a real object; however, they showed a similar tendency with those of the real object under the two viewing conditions. Therefore, IP can induce accommodation to the depth positions of 3D images.

  8. Progress in 3D imaging and display by integral imaging

    NASA Astrophysics Data System (ADS)

    Martinez-Cuenca, R.; Saavedra, G.; Martinez-Corral, M.; Pons, A.; Javidi, B.

    2009-05-01

    Three-dimensionality is currently considered an important added value in imaging devices, and therefore the search for an optimum 3D imaging and display technique is a hot topic that is attracting important research efforts. As main value, 3D monitors should provide the observers with different perspectives of a 3D scene by simply varying the head position. Three-dimensional imaging techniques have the potential to establish a future mass-market in the fields of entertainment and communications. Integral imaging (InI), which can capture true 3D color images, has been seen as the right technology to 3D viewing to audiences of more than one person. Due to the advanced degree of development, InI technology could be ready for commercialization in the coming years. This development is the result of a strong research effort performed along the past few years by many groups. Since Integral Imaging is still an emerging technology, the first aim of the "3D Imaging and Display Laboratory" at the University of Valencia, has been the realization of a thorough study of the principles that govern its operation. Is remarkable that some of these principles have been recognized and characterized by our group. Other contributions of our research have been addressed to overcome some of the classical limitations of InI systems, like the limited depth of field (in pickup and in display), the poor axial and lateral resolution, the pseudoscopic-to-orthoscopic conversion, the production of 3D images with continuous relief, or the limited range of viewing angles of InI monitors.

  9. Output-sensitive 3D line integral convolution.

    PubMed

    Falk, Martin; Weiskopf, Daniel

    2008-01-01

    We propose an output-sensitive visualization method for 3D line integral convolution (LIC) whose rendering speed is largely independent of the data set size and mostly governed by the complexity of the output on the image plane. Our approach of view-dependent visualization tightly links the LIC generation with the volume rendering of the LIC result in order to avoid the computation of unnecessary LIC points: early-ray termination and empty-space leaping techniques are used to skip the computation of the LIC integral in a lazy-evaluation approach; both ray casting and texture slicing can be used as volume-rendering techniques. The input noise is modeled in object space to allow for temporal coherence under object and camera motion. Different noise models are discussed, covering dense representations based on filtered white noise all the way to sparse representations similar to oriented LIC. Aliasing artifacts are avoided by frequency control over the 3D noise and by employing a 3D variant of MIPmapping. A range of illumination models is applied to the LIC streamlines: different codimension-2 lighting models and a novel gradient-based illumination model that relies on precomputed gradients and does not require any direct calculation of gradients after the LIC integral is evaluated. We discuss the issue of proper sampling of the LIC and volume-rendering integrals by employing a frequency-space analysis of the noise model and the precomputed gradients. Finally, we demonstrate that our visualization approach lends itself to a fast graphics processing unit (GPU) implementation that supports both steady and unsteady flow. Therefore, this 3D LIC method allows users to interactively explore 3D flow by means of high-quality, view-dependent, and adaptive LIC volume visualization. Applications to flow visualization in combination with feature extraction and focus-and-context visualization are described, a comparison to previous methods is provided, and a detailed performance

  10. Radiation Effects in 3D Integrated SOl SRAM Circuits

    DTIC Science & Technology

    2011-08-23

    Comparing Neutrons and Protons Data Monoenergetic neutrons and protons are used to characterize single event effects in electronics circuits, and are...for proton irradiation with energies between 4.8 and 500 MeV. Results are compared with 14-MeV neutron irradiation. Single event upset cross-section...fabricating circuits for space applications. singIe event effects, SOl, fully depleted, 3D integration, neutron , protons, upset cross-section U U U U SAR

  11. Integrating Quality Management Into a 3d Geospatial Server

    NASA Astrophysics Data System (ADS)

    Coors, V.; Krämer, M.

    2011-08-01

    In recent years the technology and workflow for producing and management of large 3D urban models has been established and widely been used. Standards such as CityGML enable the modelling and exchange of semantically enriched multi-purpose 3D urban models for applications like urban planning, public participation, environmental simulation and navigation. However, data quality management is essential to control and enhance the quality of these models in order to be able to meet the needs of the aforementioned applications. Quality management should be performed throughout the whole lifecycle of geospatial datasets - from data acquisition to processing, analysis and visualisation. In this paper, we therefore focus on the integration of a quality management software module into a 3D geospatial data server. First results of a prototype system developed at HFT Stuttgart together with Fraunhofer IGD will be presented in this paper as a starting point for further research into the field of quality management of 3D city models.

  12. 3D-GNOME: an integrated web service for structural modeling of the 3D genome.

    PubMed

    Szalaj, Przemyslaw; Michalski, Paul J; Wróblewski, Przemysław; Tang, Zhonghui; Kadlof, Michal; Mazzocco, Giovanni; Ruan, Yijun; Plewczynski, Dariusz

    2016-07-08

    Recent advances in high-throughput chromosome conformation capture (3C) technology, such as Hi-C and ChIA-PET, have demonstrated the importance of 3D genome organization in development, cell differentiation and transcriptional regulation. There is now a widespread need for computational tools to generate and analyze 3D structural models from 3C data. Here we introduce our 3D GeNOme Modeling Engine (3D-GNOME), a web service which generates 3D structures from 3C data and provides tools to visually inspect and annotate the resulting structures, in addition to a variety of statistical plots and heatmaps which characterize the selected genomic region. Users submit a bedpe (paired-end BED format) file containing the locations and strengths of long range contact points, and 3D-GNOME simulates the structure and provides a convenient user interface for further analysis. Alternatively, a user may generate structures using published ChIA-PET data for the GM12878 cell line by simply specifying a genomic region of interest. 3D-GNOME is freely available at http://3dgnome.cent.uw.edu.pl/.

  13. 3D-GNOME: an integrated web service for structural modeling of the 3D genome

    PubMed Central

    Szalaj, Przemyslaw; Michalski, Paul J.; Wróblewski, Przemysław; Tang, Zhonghui; Kadlof, Michal; Mazzocco, Giovanni; Ruan, Yijun; Plewczynski, Dariusz

    2016-01-01

    Recent advances in high-throughput chromosome conformation capture (3C) technology, such as Hi-C and ChIA-PET, have demonstrated the importance of 3D genome organization in development, cell differentiation and transcriptional regulation. There is now a widespread need for computational tools to generate and analyze 3D structural models from 3C data. Here we introduce our 3D GeNOme Modeling Engine (3D-GNOME), a web service which generates 3D structures from 3C data and provides tools to visually inspect and annotate the resulting structures, in addition to a variety of statistical plots and heatmaps which characterize the selected genomic region. Users submit a bedpe (paired-end BED format) file containing the locations and strengths of long range contact points, and 3D-GNOME simulates the structure and provides a convenient user interface for further analysis. Alternatively, a user may generate structures using published ChIA-PET data for the GM12878 cell line by simply specifying a genomic region of interest. 3D-GNOME is freely available at http://3dgnome.cent.uw.edu.pl/. PMID:27185892

  14. 3D J-Integral Capability in Grizzly

    SciTech Connect

    Spencer, Benjamin; Backman, Marie; Chakraborty, Pritam; Hoffman, William

    2014-09-01

    This report summarizes work done to develop a capability to evaluate fracture contour J-Integrals in 3D in the Grizzly code. In the current fiscal year, a previously-developed 2D implementation of a J-Integral evaluation capability has been extended to work in 3D, and to include terms due both to mechanically-induced strains and due to gradients in thermal strains. This capability has been verified against a benchmark solution on a model of a curved crack front in 3D. The thermal term in this integral has been verified against a benchmark problem with a thermal gradient. These developments are part of a larger effort to develop Grizzly as a tool that can be used to predict the evolution of aging processes in nuclear power plant systems, structures, and components, and assess their capacity after being subjected to those aging processes. The capabilities described here have been developed to enable evaluations of Mode- stress intensity factors on axis-aligned flaws in reactor pressure vessels. These can be compared with the fracture toughness of the material to determine whether a pre-existing flaw would begin to propagate during a pos- tulated pressurized thermal shock accident. This report includes a demonstration calculation to show how Grizzly is used to perform a deterministic assessment of such a flaw propagation in a degraded reactor pressure vessel under pressurized thermal shock conditions. The stress intensity is calculated from J, and the toughness is computed using the fracture master curve and the degraded ductile to brittle transition temperature.

  15. Customisable 3D printed microfluidics for integrated analysis and optimisation.

    PubMed

    Monaghan, T; Harding, M J; Harris, R A; Friel, R J; Christie, S D R

    2016-08-16

    The formation of smart Lab-on-a-Chip (LOC) devices featuring integrated sensing optics is currently hindered by convoluted and expensive manufacturing procedures. In this work, a series of 3D-printed LOC devices were designed and manufactured via stereolithography (SL) in a matter of hours. The spectroscopic performance of a variety of optical fibre combinations were tested, and the optimum path length for performing Ultraviolet-visible (UV-vis) spectroscopy determined. The information gained in these trials was then used in a reaction optimisation for the formation of carvone semicarbazone. The production of high resolution surface channels (100-500 μm) means that these devices were capable of handling a wide range of concentrations (9 μM-38 mM), and are ideally suited to both analyte detection and process optimisation. This ability to tailor the chip design and its integrated features as a direct result of the reaction being assessed, at such a low time and cost penalty greatly increases the user's ability to optimise both their device and reaction. As a result of the information gained in this investigation, we are able to report the first instance of a 3D-printed LOC device with fully integrated, in-line monitoring capabilities via the use of embedded optical fibres capable of performing UV-vis spectroscopy directly inside micro channels.

  16. Multi-user 3D film on a time-multiplexed side-emission backlight system.

    PubMed

    Ting, Chih-Hung; Chang, Yu-Cheng; Chen, Chun-Ho; Huang, Yi-Pai; Tsai, Han-Wen

    2016-10-01

    The desirable features for a portable 3D display include displaying 2D and 3D images without resolution degradation for multiple users, a 2D/3D switchable functionality, and, in particular, a compact volume. To produce a portable 3D display with these desirable features, we propose here a multi-user 3D film combined with a side-emission backlight system that has a directional-sequential light distribution. According to the simulation and experimental results, the multi-user 3D film successfully uses an inverted trapezoid structure to separate the rays of each light source and increases the number of observers from one to three. Additionally, the specification of the inverted trapezoid structure can be determined via equations for different designated viewing positions of the side observer and for the ratio of light intensities for the central and side observers.

  17. Integral imaging based 3D display of holographic data.

    PubMed

    Yöntem, Ali Özgür; Onural, Levent

    2012-10-22

    We propose a method and present applications of this method that converts a diffraction pattern into an elemental image set in order to display them on an integral imaging based display setup. We generate elemental images based on diffraction calculations as an alternative to commonly used ray tracing methods. Ray tracing methods do not accommodate the interference and diffraction phenomena. Our proposed method enables us to obtain elemental images from a holographic recording of a 3D object/scene. The diffraction pattern can be either numerically generated data or digitally acquired optical data. The method shows the connection between a hologram (diffraction pattern) and an elemental image set of the same 3D object. We showed three examples, one of which is the digitally captured optical diffraction tomography data of an epithelium cell. We obtained optical reconstructions with our integral imaging display setup where we used a digital lenslet array. We also obtained numerical reconstructions, again by using the diffraction calculations, for comparison. The digital and optical reconstruction results are in good agreement.

  18. An integrated microspectrometer for localised multiplexing measurements.

    PubMed

    Hu, Zhixiong; Glidle, Andrew; Ironside, Charles; Cooper, Jonathan M; Yin, Huabing

    2015-01-07

    We describe the development of an integrated lensed Arrayed Waveguide Grating (AWG) microspectrometer for localized multiplexing fluorescence measurements. The device, which has a footprint that is only 1 mm wide and 1 cm long, is capable of spectroscopic measurements on chip. Multiple fluorescence signals were measured simultaneously based upon simple intensity readouts from a CCD camera. We also demonstrate the integration of the AWG spectrometer with a microfluidic platform using a lensing function to confine the beam shape for focused illumination. This capability enhances signal collection, gives better spatial resolution, and provides a route for the analysis of small volume samples (e.g. cells) in flow. To show these capabilities we developed a novel "bead-AWG" platform with which we demonstrate localized multiplexed fluorescence detection either simultaneously or successively. Such an integrated system provides the basis for a portable system capable of optical detection of multi-wavelength fluorescence from a single defined location.

  19. Tunable quantum interference in a 3D integrated circuit.

    PubMed

    Chaboyer, Zachary; Meany, Thomas; Helt, L G; Withford, Michael J; Steel, M J

    2015-04-27

    Integrated photonics promises solutions to questions of stability, complexity, and size in quantum optics. Advances in tunable and non-planar integrated platforms, such as laser-inscribed photonics, continue to bring the realisation of quantum advantages in computation and metrology ever closer, perhaps most easily seen in multi-path interferometry. Here we demonstrate control of two-photon interference in a chip-scale 3D multi-path interferometer, showing a reduced periodicity and enhanced visibility compared to single photon measurements. Observed non-classical visibilities are widely tunable, and explained well by theoretical predictions based on classical measurements. With these predictions we extract Fisher information approaching a theoretical maximum. Our results open a path to quantum enhanced phase measurements.

  20. Simultaneous full-field 3-D vibrometry of the human eardrum using spatial-bandwidth multiplexed holography

    PubMed Central

    Khaleghi, Morteza; Guignard, Jérémie; Furlong, Cosme; Rosowski, John J.

    2015-01-01

    Abstract. Holographic interferometric methods typically require the use of three sensitivity vectors in order to obtain three-dimensional (3-D) information. Methods based on multiple directions of illumination have limited applications when studying biological tissues that have temporally varying responses such as the tympanic membrane (TM). Therefore, to measure 3-D displacements in such applications, the measurements along all the sensitivity vectors have to be done simultaneously. We propose a multiple-illumination directions approach to measure 3-D displacements from a single-shot hologram that contains displacement information from three sensitivity vectors. The hologram of an object of interest is simultaneously recorded with three incoherently superimposed pairs of reference and object beams. The incident off-axis angles of the reference beams are adjusted such that the frequency components of the multiplexed hologram are completely separate. Because of the differences in the directions and wavelengths of the reference beams, the positions of each reconstructed image corresponding to each sensitivity vector are different. We implemented a registration algorithm to accurately translate individual components of the hologram into a single global coordinate system to calculate 3-D displacements. The results include magnitudes and phases of 3-D sound-induced motions of a human cadaveric TM at several excitation frequencies showing modal and traveling wave motions on its surface. PMID:25984986

  1. The 3-D vision system integrated dexterous hand

    NASA Technical Reports Server (NTRS)

    Luo, Ren C.; Han, Youn-Sik

    1989-01-01

    Most multifingered hands use a tendon mechanism to minimize the size and weight of the hand. Such tendon mechanisms suffer from the problems of striction and friction of the tendons resulting in a reduction of control accuracy. A design for a 3-D vision system integrated dexterous hand with motor control is described which overcomes these problems. The proposed hand is composed of three three-jointed grasping fingers with tactile sensors on their tips, a two-jointed eye finger with a cross-shaped laser beam emitting diode in its distal part. The two non-grasping fingers allow 3-D vision capability and can rotate around the hand to see and measure the sides of grasped objects and the task environment. An algorithm that determines the range and local orientation of the contact surface using a cross-shaped laser beam is introduced along with some potential applications. An efficient method for finger force calculation is presented which uses the measured contact surface normals of an object.

  2. 3D plasmonic nanoantennas integrated with MEA biosensors.

    PubMed

    Dipalo, Michele; Messina, Gabriele C; Amin, Hayder; La Rocca, Rosanna; Shalabaeva, Victoria; Simi, Alessandro; Maccione, Alessandro; Zilio, Pierfrancesco; Berdondini, Luca; De Angelis, Francesco

    2015-02-28

    Neuronal signaling in brain circuits occurs at multiple scales ranging from molecules and cells to large neuronal assemblies. However, current sensing neurotechnologies are not designed for parallel access of signals at multiple scales. With the aim of combining nanoscale molecular sensing with electrical neural activity recordings within large neuronal assemblies, in this work three-dimensional (3D) plasmonic nanoantennas are integrated with multielectrode arrays (MEA). Nanoantennas are fabricated by fast ion beam milling on optical resist; gold is deposited on the nanoantennas in order to connect them electrically to the MEA microelectrodes and to obtain plasmonic behavior. The optical properties of these 3D nanostructures are studied through finite elements method (FEM) simulations that show a high electromagnetic field enhancement. This plasmonic enhancement is confirmed by surface enhancement Raman spectroscopy of a dye performed in liquid, which presents an enhancement of almost 100 times the incident field amplitude at resonant excitation. Finally, the reported MEA devices are tested on cultured rat hippocampal neurons. Neurons develop by extending branches on the nanostructured electrodes and extracellular action potentials are recorded over multiple days in vitro. Raman spectra of living neurons cultured on the nanoantennas are also acquired. These results highlight that these nanostructures could be potential candidates for combining electrophysiological measures of large networks with simultaneous spectroscopic investigations at the molecular level.

  3. 3D plasmonic nanoantennas integrated with MEA biosensors

    NASA Astrophysics Data System (ADS)

    Dipalo, Michele; Messina, Gabriele C.; Amin, Hayder; La Rocca, Rosanna; Shalabaeva, Victoria; Simi, Alessandro; Maccione, Alessandro; Zilio, Pierfrancesco; Berdondini, Luca; de Angelis, Francesco

    2015-02-01

    Neuronal signaling in brain circuits occurs at multiple scales ranging from molecules and cells to large neuronal assemblies. However, current sensing neurotechnologies are not designed for parallel access of signals at multiple scales. With the aim of combining nanoscale molecular sensing with electrical neural activity recordings within large neuronal assemblies, in this work three-dimensional (3D) plasmonic nanoantennas are integrated with multielectrode arrays (MEA). Nanoantennas are fabricated by fast ion beam milling on optical resist; gold is deposited on the nanoantennas in order to connect them electrically to the MEA microelectrodes and to obtain plasmonic behavior. The optical properties of these 3D nanostructures are studied through finite elements method (FEM) simulations that show a high electromagnetic field enhancement. This plasmonic enhancement is confirmed by surface enhancement Raman spectroscopy of a dye performed in liquid, which presents an enhancement of almost 100 times the incident field amplitude at resonant excitation. Finally, the reported MEA devices are tested on cultured rat hippocampal neurons. Neurons develop by extending branches on the nanostructured electrodes and extracellular action potentials are recorded over multiple days in vitro. Raman spectra of living neurons cultured on the nanoantennas are also acquired. These results highlight that these nanostructures could be potential candidates for combining electrophysiological measures of large networks with simultaneous spectroscopic investigations at the molecular level.Neuronal signaling in brain circuits occurs at multiple scales ranging from molecules and cells to large neuronal assemblies. However, current sensing neurotechnologies are not designed for parallel access of signals at multiple scales. With the aim of combining nanoscale molecular sensing with electrical neural activity recordings within large neuronal assemblies, in this work three-dimensional (3D) plasmonic

  4. Digital 3D Borobudur - Integration of 3D surveying and modeling techniques

    NASA Astrophysics Data System (ADS)

    Suwardhi, D.; Menna, F.; Remondino, F.; Hanke, K.; Akmalia, R.

    2015-08-01

    The Borobudur temple (Indonesia) is one of the greatest Buddhist monuments in the world, now listed as an UNESCO World Heritage Site. The present state of the temple is the result of restorations after being exposed to natural disasters several times. Today there is still a growing rate of deterioration of the building stones whose causes need further researches. Monitoring programs, supported at institutional level, have been effectively executed to observe the problem. The paper presents the latest efforts to digitally document the Borobudur Temple and its surrounding area in 3D with photogrammetric techniques. UAV and terrestrial images were acquired to completely digitize the temple, produce DEM, orthoimages and maps at 1:100 and 1:1000 scale. The results of the project are now employed by the local government organizations to manage the heritage area and plan new policies for the conservation and preservation of the UNESCO site. In order to help data management and policy makers, a web-based information system of the heritage area was also built to visualize and easily access all the data and achieved 3D results.

  5. Integration of virtual and real scenes within an integral 3D imaging environment

    NASA Astrophysics Data System (ADS)

    Ren, Jinsong; Aggoun, Amar; McCormick, Malcolm

    2002-11-01

    The Imaging Technologies group at De Montfort University has developed an integral 3D imaging system, which is seen as the most likely vehicle for 3D television avoiding psychological effects. To create real fascinating three-dimensional television programs, a virtual studio that performs the task of generating, editing and integrating the 3D contents involving virtual and real scenes is required. The paper presents, for the first time, the procedures, factors and methods of integrating computer-generated virtual scenes with real objects captured using the 3D integral imaging camera system. The method of computer generation of 3D integral images, where the lens array is modelled instead of the physical camera is described. In the model each micro-lens that captures different elemental images of the virtual scene is treated as an extended pinhole camera. An integration process named integrated rendering is illustrated. Detailed discussion and deep investigation are focused on depth extraction from captured integral 3D images. The depth calculation method from the disparity and the multiple baseline method that is used to improve the precision of depth estimation are also presented. The concept of colour SSD and its further improvement in the precision is proposed and verified.

  6. Integrating visible light 3D scanning into the everyday world

    NASA Astrophysics Data System (ADS)

    Straub, Jeremy

    2015-05-01

    Visible light 3D scanning offers the potential to non-invasively and nearly non-perceptibly incorporate 3D imaging into the everyday world. This paper considers the various possible uses of visible light 3D scanning technology. It discusses multiple possible usage scenarios including in hospitals, security perimeter settings and retail environments. The paper presents a framework for assessing the efficacy of visible light 3D scanning for a given application (and compares this to other scanning approaches such as those using blue light or lasers). It also discusses ethical and legal considerations relevant to real-world use and concludes by presenting a decision making framework.

  7. A monolithic 3D integrated nanomagnetic co-processing unit

    NASA Astrophysics Data System (ADS)

    Becherer, M.; Breitkreutz-v. Gamm, S.; Eichwald, I.; Žiemys, G.; Kiermaier, J.; Csaba, G.; Schmitt-Landsiedel, D.

    2016-01-01

    As CMOS scaling becomes more and more challenging there is strong impetus for beyond CMOS device research to add new functionality to ICs. In this article, a promising technology with non-volatile ferromagnetic computing states - the so-called Perpendicular Nanomagnetic Logic (pNML) - is reviewed. After introducing the 2D planar implementation of NML with magnetization perpendicular to the surface, the path to monolithically 3D integrated systems is discussed. Instead of CMOS substitution, additional functionality is added by a co-processor architecture as a prospective back-end-of-line (BEOL) process, where the computing elements are clocked by a soft-magnetic on-chip inductor. The unconventional computation in the ferromagnetic domain can lead to highly dense computing structures without leakage currents, attojoule dissipation per bit operation and data-throughputs comparable to state-of-the-art high-performance CMOS CPUs. In appropriate applications and with specialized computing architectures they might even circumvent the bottleneck of time-consuming memory access, as computation is inherently performed with non-volatile computing states.

  8. Integrating 3D Visualization and GIS in Planning Education

    ERIC Educational Resources Information Center

    Yin, Li

    2010-01-01

    Most GIS-related planning practices and education are currently limited to two-dimensional mapping and analysis although 3D GIS is a powerful tool to study the complex urban environment in its full spatial extent. This paper reviews current GIS and 3D visualization uses and development in planning practice and education. Current literature…

  9. 3D Vectorial Time Domain Computational Integrated Photonics

    SciTech Connect

    Kallman, J S; Bond, T C; Koning, J M; Stowell, M L

    2007-02-16

    The design of integrated photonic structures poses considerable challenges. 3D-Time-Domain design tools are fundamental in enabling technologies such as all-optical logic, photonic bandgap sensors, THz imaging, and fast radiation diagnostics. Such technologies are essential to LLNL and WFO sponsors for a broad range of applications: encryption for communications and surveillance sensors (NSA, NAI and IDIV/PAT); high density optical interconnects for high-performance computing (ASCI); high-bandwidth instrumentation for NIF diagnostics; micro-sensor development for weapon miniaturization within the Stockpile Stewardship and DNT programs; and applications within HSO for CBNP detection devices. While there exist a number of photonics simulation tools on the market, they primarily model devices of interest to the communications industry. We saw the need to extend our previous software to match the Laboratory's unique emerging needs. These include modeling novel material effects (such as those of radiation induced carrier concentrations on refractive index) and device configurations (RadTracker bulk optics with radiation induced details, Optical Logic edge emitting lasers with lateral optical inputs). In addition we foresaw significant advantages to expanding our own internal simulation codes: parallel supercomputing could be incorporated from the start, and the simulation source code would be accessible for modification and extension. This work addressed Engineering's Simulation Technology Focus Area, specifically photonics. Problems addressed from the Engineering roadmap of the time included modeling the Auston switch (an important THz source/receiver), modeling Vertical Cavity Surface Emitting Lasers (VCSELs, which had been envisioned as part of fast radiation sensors), and multi-scale modeling of optical systems (for a variety of applications). We proposed to develop novel techniques to numerically solve the 3D multi-scale propagation problem for both the microchip

  10. Single DMD time-multiplexed 64-views autostereoscopic 3D display

    NASA Astrophysics Data System (ADS)

    Loreti, Luigi

    2013-03-01

    Based on previous prototype of the Real time 3D holographic display developed last year, we developed a new concept of auto-stereoscopic multiview display (64 views), wide angle (90°) 3D full color display. The display is based on a RGB laser light source illuminating a DMD (Discovery 4100 0,7") at 24.000 fps, an image deflection system made with an AOD (Acoustic Optic Deflector) driven by a piezo-electric transducer generating a variable standing acoustic wave on the crystal that acts as a phase grating. The DMD projects in fast sequence 64 point of view of the image on the crystal cube. Depending on the frequency of the standing wave, the input picture sent by the DMD is deflected in different angle of view. An holographic screen at a proper distance diffuse the rays in vertical direction (60°) and horizontally select (1°) only the rays directed to the observer. A telescope optical system will enlarge the image to the right dimension. A VHDL firmware to render in real-time (16 ms) 64 views (16 bit 4:2:2) of a CAD model (obj, dxf or 3Ds) and depth-map encoded video images was developed into the resident Virtex5 FPGA of the Discovery 4100 SDK, thus eliminating the needs of image transfer and high speed links

  11. BioMEA: a versatile high-density 3D microelectrode array system using integrated electronics.

    PubMed

    Charvet, Guillaume; Rousseau, Lionel; Billoint, Olivier; Gharbi, Sadok; Rostaing, Jean-Pierre; Joucla, Sébastien; Trevisiol, Michel; Bourgerette, Alain; Chauvet, Philippe; Moulin, Céline; Goy, François; Mercier, Bruno; Colin, Mikael; Spirkovitch, Serge; Fanet, Hervé; Meyrand, Pierre; Guillemaud, Régis; Yvert, Blaise

    2010-04-15

    Microelectrode arrays (MEAs) offer a powerful tool to both record activity and deliver electrical microstimulations to neural networks either in vitro or in vivo. Microelectronics microfabrication technologies now allow building high-density MEAs containing several hundreds of microelectrodes. However, dense arrays of 3D micro-needle electrodes, providing closer contact with the neural tissue than planar electrodes, are not achievable using conventional isotropic etching processes. Moreover, increasing the number of electrodes using conventional electronics is difficult to achieve into compact devices addressing all channels independently for simultaneous recording and stimulation. Here, we present a full modular and versatile 256-channel MEA system based on integrated electronics. First, transparent high-density arrays of 3D-shaped microelectrodes were realized by deep reactive ion etching techniques of a silicon substrate reported on glass. This approach allowed achieving high electrode aspect ratios, and different shapes of tip electrodes. Next, we developed a dedicated analog 64-channel Application Specific Integrated Circuit (ASIC) including one amplification stage and one current generator per channel, and analog output multiplexing. A full modular system, called BIOMEA, has been designed, allowing connecting different types of MEAs (64, 128, or 256 electrodes) to different numbers of ASICs for simultaneous recording and/or stimulation on all channels. Finally, this system has been validated experimentally by recording and electrically eliciting low-amplitude spontaneous rhythmic activity (both LFPs and spikes) in the developing mouse CNS. The availability of high-density MEA systems with integrated electronics will offer new possibilities for both in vitro and in vivo studies of large neural networks.

  12. Integration of real-time 3D capture, reconstruction, and light-field display

    NASA Astrophysics Data System (ADS)

    Zhang, Zhaoxing; Geng, Zheng; Li, Tuotuo; Pei, Renjing; Liu, Yongchun; Zhang, Xiao

    2015-03-01

    Effective integration of 3D acquisition, reconstruction (modeling) and display technologies into a seamless systems provides augmented experience of visualizing and analyzing real objects and scenes with realistic 3D sensation. Applications can be found in medical imaging, gaming, virtual or augmented reality and hybrid simulations. Although 3D acquisition, reconstruction, and display technologies have gained significant momentum in recent years, there seems a lack of attention on synergistically combining these components into a "end-to-end" 3D visualization system. We designed, built and tested an integrated 3D visualization system that is able to capture in real-time 3D light-field images, perform 3D reconstruction to build 3D model of the objects, and display the 3D model on a large autostereoscopic screen. In this article, we will present our system architecture and component designs, hardware/software implementations, and experimental results. We will elaborate on our recent progress on sparse camera array light-field 3D acquisition, real-time dense 3D reconstruction, and autostereoscopic multi-view 3D display. A prototype is finally presented with test results to illustrate the effectiveness of our proposed integrated 3D visualization system.

  13. Galerkin Boundary Integral Analysis for the 3D Helmholtz Equation

    SciTech Connect

    Swager, Melissa; Gray, Leonard J; Nintcheu Fata, Sylvain

    2010-01-01

    A linear element Galerkin boundary integral analysis for the three-dimensional Helmholtz equation is presented. The emphasis is on solving acoustic scattering by an open (crack) surface, and to this end both a dual equation formulation and a symmetric hypersingular formulation have been developed. All singular integrals are defined and evaluated via a boundary limit process, facilitating the evaluation of the (finite) hypersingular Galerkin integral. This limit process is also the basis for the algorithm for post-processing of the surface gradient. The analytic integrations required by the limit process are carried out by employing a Taylor series expansion for the exponential factor in the Helmholtz fundamental solutions. For the open surface, the implementations are validated by comparing the numerical results obtained by using the two different methods.

  14. 3D integral imaging using diffractive Fresnel lens arrays.

    PubMed

    Hain, Mathias; von Spiegel, Wolff; Schmiedchen, Marc; Tschudi, Theo; Javidi, Bahram

    2005-01-10

    We present experimental results with binary amplitude Fresnel lens arrays and binary phase Fresnel lens arrays used to implement integral imaging systems. Their optical performance is compared with high quality refractive microlens arrays and pinhole arrays in terms of image quality, color distortion and contrast. Additionally, we show the first experimental results of lens arrays with different focal lengths in integral imaging, and discuss their ability to simultaneously increase both the depth of focus and the field of view.

  15. Dual-view integral imaging 3D display using polarizer parallax barriers.

    PubMed

    Wu, Fei; Wang, Qiong-Hua; Luo, Cheng-Gao; Li, Da-Hai; Deng, Huan

    2014-04-01

    We propose a dual-view integral imaging (DVII) 3D display using polarizer parallax barriers (PPBs). The DVII 3D display consists of a display panel, a microlens array, and two PPBs. The elemental images (EIs) displayed on the left and right half of the display panel are captured from two different 3D scenes, respectively. The lights emitted from two kinds of EIs are modulated by the left and right half of the microlens array to present two different 3D images, respectively. A prototype of the DVII 3D display is developed, and the experimental results agree well with the theory.

  16. Integrated Avionics System (IAS), Integrating 3-D Technology On A Spacecraft Panel

    NASA Technical Reports Server (NTRS)

    Hunter, Don J.; Halpert, Gerald

    1999-01-01

    As spacecraft designs converge toward miniaturization, and with the volumetric and mass challenges placed on avionics, programs will continue to advance the "state of the art" in spacecraft system development with new challenges to reduce power, mass and volume. Traditionally, the trend is to focus on high-density 3-D packaging technologies. Industry has made significant progress in 3-D technologies, and other related internal and external interconnection schemes. Although new technologies have improved packaging densities, a system packaging architecture is required that not only reduces spacecraft volume and mass budgets, but increase integration efficiencies, provide modularity and flexibility to accommodate multiple missions while maintaining a low recurring cost. With these challenges in mind, a novel system packaging approach incorporates solutions that provide broader environmental applications, more flexible system interconnectivity, scalability, and simplified assembly test and integration schemes. The Integrated Avionics System (IAS) provides for a low-mass, modular distributed or centralized packaging architecture which combines ridged-flex technologies, high-density COTS hardware and a new 3-D mechanical packaging approach, Horizontal Mounted Cube (HMC). This paper will describe the fundamental elements of the IAS, HMC hardware design, system integration and environmental test results.

  17. On the Implementation of 3D Galerkin Boundary Integral Equations

    SciTech Connect

    Nintcheu Fata, Sylvain; Gray, Leonard J

    2010-01-01

    In this article, a reverse contribution technique is proposed to accelerate the construction of the dense influence matrices associated with a Galerkin approximation of singular and hypersingular boundary integral equations of mixed-type in potential theory. In addition, a general-purpose sparse preconditioner for boundary element methods has also been developed to successfully deal with ill-conditioned linear systems arising from the discretization of mixed boundary-value problems on non-smooth surfaces. The proposed preconditioner, which originates from the precorrected-FFT method, is sparse, easy to generate and apply in a Krylov subspace iterative solution of discretized boundary integral equations. Moreover, an approximate inverse of the preconditioner is implicitly built by employing an incomplete LU factorization. Numerical experiments involving mixed boundary-value problems for the Laplace equation are included to illustrate the performance and validity of the proposed techniques.

  18. Three-Dimensional Integrated Circuit (3D IC) Key Technology: Through-Silicon Via (TSV).

    PubMed

    Shen, Wen-Wei; Chen, Kuan-Neng

    2017-12-01

    3D integration with through-silicon via (TSV) is a promising candidate to perform system-level integration with smaller package size, higher interconnection density, and better performance. TSV fabrication is the key technology to permit communications between various strata of the 3D integration system. TSV fabrication steps, such as etching, isolation, metallization processes, and related failure modes, as well as other characterizations are discussed in this invited review paper.

  19. Three-Dimensional Integrated Circuit (3D IC) Key Technology: Through-Silicon Via (TSV)

    NASA Astrophysics Data System (ADS)

    Shen, Wen-Wei; Chen, Kuan-Neng

    2017-01-01

    3D integration with through-silicon via (TSV) is a promising candidate to perform system-level integration with smaller package size, higher interconnection density, and better performance. TSV fabrication is the key technology to permit communications between various strata of the 3D integration system. TSV fabrication steps, such as etching, isolation, metallization processes, and related failure modes, as well as other characterizations are discussed in this invited review paper.

  20. Display of travelling 3D scenes from single integral-imaging capture

    NASA Astrophysics Data System (ADS)

    Martinez-Corral, Manuel; Dorado, Adrian; Hong, Seok-Min; Sola-Pikabea, Jorge; Saavedra, Genaro

    2016-06-01

    Integral imaging (InI) is a 3D auto-stereoscopic technique that captures and displays 3D images. We present a method for easily projecting the information recorded with this technique by transforming the integral image into a plenoptic image, as well as choosing, at will, the field of view (FOV) and the focused plane of the displayed plenoptic image. Furthermore, with this method we can generate a sequence of images that simulates a camera travelling through the scene from a single integral image. The application of this method permits to improve the quality of 3D display images and videos.

  1. An approach for 3D geoscientific data integration in underground planning

    NASA Astrophysics Data System (ADS)

    Zhong, Zheng; Tor, Yam Khoon; Tan, Guoxian

    2008-12-01

    Due to finite space, there is an increasing need to plan and develop strategic underground facilities and infrastructures for various military and non-military applications in Singapore in recent years. The awareness of the underground option among planners, developers, and financiers should be increased so that subsurface planning issues can be better addressed. The lack of adequate and accurate 3D spatial data often makes the design and construction of such underground works difficult. It is necessary to integrate all of the spatial objects for underground planning. Over the past two decades, a number of commercial software systems have been developed for 3D geographic and geological modeling. For example, VGEGIS software allows users to create 3D surface geological maps. 3D GeoModeller, a 3D geological modeling and geophysical inversion package, allows project geologists to build realistic 3D geology models. This paper presents an approach to integrate the geographic and geological models for underground planning. A prototype of 3D Geographic Information System (3DGIS) called "3DRock" has been developed by authors to implement the data integration with 3D GeoModeler. The results so far showed that 3DRock is able to integrate the above-surface, surface, and subsurface information available from maps, sections, terrain models, topographic data, drillholes, etc. for the Banyan Basin in Jurong Island, Singapore, in a case study.

  2. Experiment for Integrating Dutch 3d Spatial Planning and Bim for Checking Building Permits

    NASA Astrophysics Data System (ADS)

    van Berlo, L.; Dijkmans, T.; Stoter, J.

    2013-09-01

    This paper presents a research project in The Netherlands in which several SMEs collaborated to create a 3D model of the National spatial planning information. This 2D information system described in the IMRO data standard holds implicit 3D information that can be used to generate an explicit 3D model. The project realized a proof of concept to generate a 3D spatial planning model. The team used the model to integrate it with several 3D Building Information Models (BIMs) described in the open data standard Industry Foundation Classes (IFC). Goal of the project was (1) to generate a 3D BIM model from spatial planning information to be used by the architect during the early design phase, and (2) allow 3D checking of building permits. The team used several technologies like CityGML, BIM clash detection and GeoBIM to explore the potential of this innovation. Within the project a showcase was created with a part of the spatial plan from the city of The Hague. Several BIM models were integrated in the 3D spatial plan of this area. A workflow has been described that demonstrates the benefits of collaboration between the spatial domain and the AEC industry in 3D. The research results in a showcase with conclusions and considerations for both national and international practice.

  3. Monolithically integrated reconfigurable add-drop multiplexer for mode-division-multiplexing systems.

    PubMed

    Wang, Shipeng; Wu, Hao; Tsang, Hon Ki; Dai, Daoxin

    2016-11-15

    An integrated reconfigurable optical add-drop multiplexer (ROADM) for mode-division-multiplexing systems is proposed and demonstrated for the first time, to the best of our knowledge. The present ROADM with four mode-channels is composed of a four-channel mode demultiplexer, four identical 2×2 thermo-optic Mach-Zehnder switches (MZSs), and a four-channel mode multiplexer, which are integrated monolithically on silicon. All the devices are designed for operation with TM polarization. The ROADM can add/drop any one of the mode channels freely by thermally turning on/off the corresponding MZS. For the added/dropped mode-channels, the excess loss is 1-5 dB, and the extinction ratio is 15-20 dB in the wavelength range of 1535-1565 nm.

  4. Dielectric Spectroscopic Detection of Early Failures in 3-D Integrated Circuits

    PubMed Central

    Okoro, C. A.; Ahn, Jung-Joon; You, Lin; Kopanski, Joseph J.

    2015-01-01

    The commercial introduction of three dimensional integrated circuits (3D-ICs) has been hindered by reliability challenges, such as stress related failures, resistivity changes, and unexplained early failures. In this paper, we discuss a new RF-based metrology, based on dielectric spectroscopy, for detecting and characterizing electrically active defects in fully integrated 3D devices. These defects are traceable to the chemistry of the insolation dielectrics used in the through silicon via (TSV) construction. We show that these defects may be responsible for some of the unexplained early reliability failures observed in TSV enabled 3D devices. PMID:26664695

  5. Air-touch interaction system for integral imaging 3D display

    NASA Astrophysics Data System (ADS)

    Dong, Han Yuan; Xiang, Lee Ming; Lee, Byung Gook

    2016-07-01

    In this paper, we propose an air-touch interaction system for the tabletop type integral imaging 3D display. This system consists of the real 3D image generation system based on integral imaging technique and the interaction device using a real-time finger detection interface. In this system, we used multi-layer B-spline surface approximation to detect the fingertip and gesture easily in less than 10cm height from the screen via input the hand image. The proposed system can be used in effective human computer interaction method for the tabletop type 3D display.

  6. Estimation of the degree of polarization in low-light 3D integral imaging

    NASA Astrophysics Data System (ADS)

    Carnicer, Artur; Javidi, Bahram

    2016-06-01

    The calculation of the Stokes Parameters and the Degree of Polarization in 3D integral images requires a careful manipulation of the polarimetric elemental images. This fact is particularly important if the scenes are taken in low-light conditions. In this paper, we show that the Degree of Polarization can be effectively estimated even when elemental images are recorded with few photons. The original idea was communicated in [A. Carnicer and B. Javidi, "Polarimetric 3D integral imaging in photon-starved conditions," Opt. Express 23, 6408-6417 (2015)]. First, we use the Maximum Likelihood Estimation approach for generating the 3D integral image. Nevertheless, this method produces very noisy images and thus, the degree of polarization cannot be calculated. We suggest using a Total Variation Denoising filter as a way to improve the quality of the generated 3D images. As a result, noise is suppressed but high frequency information is preserved. Finally, the degree of polarization is obtained successfully.

  7. Integrated mode converter for mode division multiplexing

    NASA Astrophysics Data System (ADS)

    Perez-Galacho, Diego; Alonso-Ramos, Carlos Alberto; Marris-Morini, Delphine; Vakarin, Vladyslav; Le Roux, Xavier; Ortega-Moñux, Alejandro; Wangüemert-Perez, Juan Gonzalo; Vivien, Laurent

    2016-05-01

    The ever growing demands of bandwidth in optical communication systems are making traditional Wavelength Division Multiplexing (WDM) based systems to reach its limit. In order to cope with future bandwidth demand is necessary to use new levels of orthogonality, such as the waveguide mode or the polarization state. Mode Division Multiplexing (MDM) has recently attracted attention as a possible solution to increase aggregate bandwidth. In this work we discuss the proposition a of mode converter that can cover the whole C-Band of optical communications. The Mode Converter is based on two Multimode Interference (MMI) couplers and a phase shifter. Insertion loss (IL) below 0.2 dB and Extinction ratio (ER) higher than 20 dB in a broad bandwidth range of 1.5 μm to 1.6 μm have been estimated. The total length of the device is less than 30 μm.

  8. Integration of 3D anatomical data obtained by CT imaging and 3D optical scanning for computer aided implant surgery

    PubMed Central

    2011-01-01

    Background A precise placement of dental implants is a crucial step to optimize both prosthetic aspects and functional constraints. In this context, the use of virtual guiding systems has been recognized as a fundamental tool to control the ideal implant position. In particular, complex periodontal surgeries can be performed using preoperative planning based on CT data. The critical point of the procedure relies on the lack of accuracy in transferring CT planning information to surgical field through custom-made stereo-lithographic surgical guides. Methods In this work, a novel methodology is proposed for monitoring loss of accuracy in transferring CT dental information into periodontal surgical field. The methodology is based on integrating 3D data of anatomical (impression and cast) and preoperative (radiographic template) models, obtained by both CT and optical scanning processes. Results A clinical case, relative to a fully edentulous jaw patient, has been used as test case to assess the accuracy of the various steps concurring in manufacturing surgical guides. In particular, a surgical guide has been designed to place implants in the bone structure of the patient. The analysis of the results has allowed the clinician to monitor all the errors, which have been occurring step by step manufacturing the physical templates. Conclusions The use of an optical scanner, which has a higher resolution and accuracy than CT scanning, has demonstrated to be a valid support to control the precision of the various physical models adopted and to point out possible error sources. A case study regarding a fully edentulous patient has confirmed the feasibility of the proposed methodology. PMID:21338504

  9. Integration of Jeddah Historical BIM and 3D GIS for Documentation and Restoration of Historical Monument

    NASA Astrophysics Data System (ADS)

    Baik, A.; Yaagoubi, R.; Boehm, J.

    2015-08-01

    This work outlines a new approach for the integration of 3D Building Information Modelling and the 3D Geographic Information System (GIS) to provide semantically rich models, and to get the benefits from both systems to help document and analyse cultural heritage sites. Our proposed framework is based on the Jeddah Historical Building Information Modelling process (JHBIM). This JHBIM consists of a Hijazi Architectural Objects Library (HAOL) that supports higher level of details (LoD) while decreasing the time of modelling. The Hijazi Architectural Objects Library has been modelled based on the Islamic historical manuscripts and Hijazi architectural pattern books. Moreover, the HAOL is implemented using BIM software called Autodesk Revit. However, it is known that this BIM environment still has some limitations with the non-standard architectural objects. Hence, we propose to integrate the developed 3D JHBIM with 3D GIS for more advanced analysis. To do so, the JHBIM database is exported and semantically enriched with non-architectural information that is necessary for restoration and preservation of historical monuments. After that, this database is integrated with the 3D Model in the 3D GIS solution. At the end of this paper, we'll illustrate our proposed framework by applying it to a Historical Building called Nasif Historical House in Jeddah. First of all, this building is scanned by the use of a Terrestrial Laser Scanner (TLS) and Close Range Photogrammetry. Then, the 3D JHBIM based on the HOAL is designed on Revit Platform. Finally, this model is integrated to a 3D GIS solution through Autodesk InfraWorks. The shown analysis presented in this research highlights the importance of such integration especially for operational decisions and sharing the historical knowledge about Jeddah Historical City. Furthermore, one of the historical buildings in Old Jeddah, Nasif Historical House, was chosen as a test case for the project.

  10. Bi-sided integral imaging with 2D/3D convertibility using scattering polarizer.

    PubMed

    Yeom, Jiwoon; Hong, Keehoon; Park, Soon-gi; Hong, Jisoo; Min, Sung-Wook; Lee, Byoungho

    2013-12-16

    We propose a two-dimensional (2D) and three-dimensional (3D) convertible bi-sided integral imaging. The proposed system uses the polarization state of projected light for switching its operation mode between 2D and 3D modes. By using an optical module composed of two scattering polarizers and one linear polarizer, the proposed integral imaging system simultaneously provides 3D images with 2D background images for observers who are located in the front and the rear sides of the system. The occlusion effect between 2D images and 3D images is realized by using a compensation mask for 2D images and the elemental images. The principle of proposed system is experimentally verified.

  11. An architecture for integrating planar and 3D cQED devices

    NASA Astrophysics Data System (ADS)

    Axline, C.; Reagor, M.; Heeres, R.; Reinhold, P.; Wang, C.; Shain, K.; Pfaff, W.; Chu, Y.; Frunzio, L.; Schoelkopf, R. J.

    2016-07-01

    Numerous loss mechanisms can limit coherence and scalability of planar and 3D-based circuit quantum electrodynamics (cQED) devices, particularly due to their packaging. The low loss and natural isolation of 3D enclosures make them good candidates for coherent scaling. We introduce a coaxial transmission line device architecture with coherence similar to traditional 3D cQED systems. Measurements demonstrate well-controlled external and on-chip couplings, a spectrum absent of cross-talk or spurious modes, and excellent resonator and qubit lifetimes. We integrate a resonator-qubit system in this architecture with a seamless 3D cavity, and separately pattern a qubit, readout resonator, Purcell filter, and high-Q stripline resonator on a single chip. Device coherence and its ease of integration make this a promising tool for complex experiments.

  12. Free segmentation in rendered 3D images through synthetic impulse response in integral imaging

    NASA Astrophysics Data System (ADS)

    Martínez-Corral, M.; Llavador, A.; Sánchez-Ortiga, E.; Saavedra, G.; Javidi, B.

    2016-06-01

    Integral Imaging is a technique that has the capability of providing not only the spatial, but also the angular information of three-dimensional (3D) scenes. Some important applications are the 3D display and digital post-processing as for example, depth-reconstruction from integral images. In this contribution we propose a new reconstruction method that takes into account the integral image and a simplified version of the impulse response function (IRF) of the integral imaging (InI) system to perform a two-dimensional (2D) deconvolution. The IRF of an InI system has a periodic structure that depends directly on the axial position of the object. Considering different periods of the IRFs we recover by deconvolution the depth information of the 3D scene. An advantage of our method is that it is possible to obtain nonconventional reconstructions by considering alternative synthetic impulse responses. Our experiments show the feasibility of the proposed method.

  13. On the integrability of the motion of 3D-Swinging Atwood machine and related problems

    NASA Astrophysics Data System (ADS)

    Elmandouh, A. A.

    2016-03-01

    In the present article, we study the problem of the motion of 3D- Swinging Atwood machine. A new integrable case for this problem is announced. We point out a new integrable case describing the motion of a heavy particle on a titled cone.

  14. Learning from graphically integrated 2D and 3D representations improves retention of neuroanatomy

    NASA Astrophysics Data System (ADS)

    Naaz, Farah

    Visualizations in the form of computer-based learning environments are highly encouraged in science education, especially for teaching spatial material. Some spatial material, such as sectional neuroanatomy, is very challenging to learn. It involves learning the two dimensional (2D) representations that are sampled from the three dimensional (3D) object. In this study, a computer-based learning environment was used to explore the hypothesis that learning sectional neuroanatomy from a graphically integrated 2D and 3D representation will lead to better learning outcomes than learning from a sequential presentation. The integrated representation explicitly demonstrates the 2D-3D transformation and should lead to effective learning. This study was conducted using a computer graphical model of the human brain. There were two learning groups: Whole then Sections, and Integrated 2D3D. Both groups learned whole anatomy (3D neuroanatomy) before learning sectional anatomy (2D neuroanatomy). The Whole then Sections group then learned sectional anatomy using 2D representations only. The Integrated 2D3D group learned sectional anatomy from a graphically integrated 3D and 2D model. A set of tests for generalization of knowledge to interpreting biomedical images was conducted immediately after learning was completed. The order of presentation of the tests of generalization of knowledge was counterbalanced across participants to explore a secondary hypothesis of the study: preparation for future learning. If the computer-based instruction programs used in this study are effective tools for teaching anatomy, the participants should continue learning neuroanatomy with exposure to new representations. A test of long-term retention of sectional anatomy was conducted 4-8 weeks after learning was completed. The Integrated 2D3D group was better than the Whole then Sections

  15. Fabrication of nano-scale Cu bond pads with seal design in 3D integration applications.

    PubMed

    Chen, K N; Tsang, C K; Wu, W W; Lee, S H; Lu, J Q

    2011-04-01

    A method to fabricate nano-scale Cu bond pads for improving bonding quality in 3D integration applications is reported. The effect of Cu bonding quality on inter-level via structural reliability for 3D integration applications is investigated. We developed a Cu nano-scale-height bond pad structure and fabrication process for improved bonding quality by recessing oxides using a combination of SiO2 CMP process and dilute HF wet etching. In addition, in order to achieve improved wafer-level bonding, we introduced a seal design concept that prevents corrosion and provides extra mechanical support. Demonstrations of these concepts and processes provide the feasibility of reliable nano-scale 3D integration applications.

  16. Dual-view integral imaging 3D display by using orthogonal polarizer array and polarization switcher.

    PubMed

    Wang, Qiong-Hua; Ji, Chao-Chao; Li, Lei; Deng, Huan

    2016-01-11

    In this paper, a dual-view integral imaging three-dimensional (3D) display consisting of a display panel, two orthogonal polarizer arrays, a polarization switcher, and a micro-lens array is proposed. Two elemental image arrays for two different 3D images are presented by the display panel alternately, and the polarization switcher controls the polarization direction of the light rays synchronously. The two elemental image arrays are modulated by their corresponding and neighboring micro-lenses of the micro-lens array, and reconstruct two different 3D images in viewing zones 1 and 2, respectively. A prototype of the dual-view II 3D display is developed, and it has good performances.

  17. Strategies for Effectively Visualizing a 3D Flow Using Volume Line Integral Convolution

    NASA Technical Reports Server (NTRS)

    Interrante, Victoria; Grosch, Chester

    1997-01-01

    This paper discusses strategies for effectively portraying 3D flow using volume line integral convolution. Issues include defining an appropriate input texture, clarifying the distinct identities and relative depths of the advected texture elements, and selectively highlighting regions of interest in both the input and output volumes. Apart from offering insights into the greater potential of 3D LIC as a method for effectively representing flow in a volume, a principal contribution of this work is the suggestion of a technique for generating and rendering 3D visibility-impeding 'halos' that can help to intuitively indicate the presence of depth discontinuities between contiguous elements in a projection and thereby clarify the 3D spatial organization of elements in the flow. The proposed techniques are applied to the visualization of a hot, supersonic, laminar jet exiting into a colder, subsonic coflow.

  18. 3D Printed Microfluidic Device with Integrated Biosensors for Online Analysis of Subcutaneous Human Microdialysate

    PubMed Central

    2015-01-01

    This work presents the design, fabrication, and characterization of a robust 3D printed microfluidic analysis system that integrates with FDA-approved clinical microdialysis probes for continuous monitoring of human tissue metabolite levels. The microfluidic device incorporates removable needle type integrated biosensors for glucose and lactate, which are optimized for high tissue concentrations, housed in novel 3D printed electrode holders. A soft compressible 3D printed elastomer at the base of the holder ensures a good seal with the microfluidic chip. Optimization of the channel size significantly improves the response time of the sensor. As a proof-of-concept study, our microfluidic device was coupled to lab-built wireless potentiostats and used to monitor real-time subcutaneous glucose and lactate levels in cyclists undergoing a training regime. PMID:26070023

  19. Extended field-of-view and increased-signal 3D holographic illumination with time-division multiplexing.

    PubMed

    Yang, Samuel J; Allen, William E; Kauvar, Isaac; Andalman, Aaron S; Young, Noah P; Kim, Christina K; Marshel, James H; Wetzstein, Gordon; Deisseroth, Karl

    2015-12-14

    Phase spatial light modulators (SLMs) are widely used for generating multifocal three-dimensional (3D) illumination patterns, but these are limited to a field of view constrained by the pixel count or size of the SLM. Further, with two-photon SLM-based excitation, increasing the number of focal spots penalizes the total signal linearly--requiring more laser power than is available or can be tolerated by the sample. Here we analyze and demonstrate a method of using galvanometer mirrors to time-sequentially reposition multiple 3D holograms, both extending the field of view and increasing the total time-averaged two-photon signal. We apply our approach to 3D two-photon in vivo neuronal calcium imaging.

  20. Extended field-of-view and increased-signal 3D holographic illumination with time-division multiplexing

    PubMed Central

    Yang, Samuel J.; Allen, William E.; Kauvar, Isaac; Andalman, Aaron S.; Young, Noah P.; Kim, Christina K.; Marshel, James H.; Wetzstein, Gordon; Deisseroth, Karl

    2016-01-01

    Phase spatial light modulators (SLMs) are widely used for generating multifocal three-dimensional (3D) illumination patterns, but these are limited to a field of view constrained by the pixel count or size of the SLM. Further, with two-photon SLM-based excitation, increasing the number of focal spots penalizes the total signal linearly—requiring more laser power than is available or can be tolerated by the sample. Here we analyze and demonstrate a method of using galvanometer mirrors to time-sequentially reposition multiple 3D holograms, both extending the field of view and increasing the total time-averaged two-photon signal. We apply our approach to 3D two-photon in vivo neuronal calcium imaging. PMID:26699047

  1. Novel fully integrated computer system for custom footwear: from 3D digitization to manufacturing

    NASA Astrophysics Data System (ADS)

    Houle, Pascal-Simon; Beaulieu, Eric; Liu, Zhaoheng

    1998-03-01

    This paper presents a recently developed custom footwear system, which integrates 3D digitization technology, range image fusion techniques, a 3D graphical environment for corrective actions, parametric curved surface representation and computer numerical control (CNC) machining. In this system, a support designed with the help of biomechanics experts can stabilize the foot in a correct and neutral position. The foot surface is then captured by a 3D camera using active ranging techniques. A software using a library of documented foot pathologies suggests corrective actions on the orthosis. Three kinds of deformations can be achieved. The first method uses previously scanned pad surfaces by our 3D scanner, which can be easily mapped onto the foot surface to locally modify the surface shape. The second kind of deformation is construction of B-Spline surfaces by manipulating control points and modifying knot vectors in a 3D graphical environment to build desired deformation. The last one is a manual electronic 3D pen, which may be of different shapes and sizes, and has an adjustable 'pressure' information. All applied deformations should respect a G1 surface continuity, which ensure that the surface can accustom a foot. Once the surface modification process is completed, the resulting data is sent to manufacturing software for CNC machining.

  2. Large scale 3-D modeling by integration of resistivity models and borehole data through inversion

    NASA Astrophysics Data System (ADS)

    Foged, N.; Marker, P. A.; Christansen, A. V.; Bauer-Gottwein, P.; Jørgensen, F.; Høyer, A.-S.; Auken, E.

    2014-02-01

    We present an automatic method for parameterization of a 3-D model of the subsurface, integrating lithological information from boreholes with resistivity models through an inverse optimization, with the objective of further detailing for geological models or as direct input to groundwater models. The parameter of interest is the clay fraction, expressed as the relative length of clay-units in a depth interval. The clay fraction is obtained from lithological logs and the clay fraction from the resistivity is obtained by establishing a simple petrophysical relationship, a translator function, between resistivity and the clay fraction. Through inversion we use the lithological data and the resistivity data to determine the optimum spatially distributed translator function. Applying the translator function we get a 3-D clay fraction model, which holds information from the resistivity dataset and the borehole dataset in one variable. Finally, we use k means clustering to generate a 3-D model of the subsurface structures. We apply the concept to the Norsminde survey in Denmark integrating approximately 700 boreholes and more than 100 000 resistivity models from an airborne survey in the parameterization of the 3-D model covering 156 km2. The final five-cluster 3-D model differentiates between clay materials and different high resistive materials from information held in resistivity model and borehole observations respectively.

  3. Large-scale 3-D modeling by integration of resistivity models and borehole data through inversion

    NASA Astrophysics Data System (ADS)

    Foged, N.; Marker, P. A.; Christansen, A. V.; Bauer-Gottwein, P.; Jørgensen, F.; Høyer, A.-S.; Auken, E.

    2014-11-01

    We present an automatic method for parameterization of a 3-D model of the subsurface, integrating lithological information from boreholes with resistivity models through an inverse optimization, with the objective of further detailing of geological models, or as direct input into groundwater models. The parameter of interest is the clay fraction, expressed as the relative length of clay units in a depth interval. The clay fraction is obtained from lithological logs and the clay fraction from the resistivity is obtained by establishing a simple petrophysical relationship, a translator function, between resistivity and the clay fraction. Through inversion we use the lithological data and the resistivity data to determine the optimum spatially distributed translator function. Applying the translator function we get a 3-D clay fraction model, which holds information from the resistivity data set and the borehole data set in one variable. Finally, we use k-means clustering to generate a 3-D model of the subsurface structures. We apply the procedure to the Norsminde survey in Denmark, integrating approximately 700 boreholes and more than 100 000 resistivity models from an airborne survey in the parameterization of the 3-D model covering 156 km2. The final five-cluster 3-D model differentiates between clay materials and different high-resistivity materials from information held in the resistivity model and borehole observations, respectively.

  4. Novel through-silicon vias for enhanced signal integrity in 3D integrated systems

    NASA Astrophysics Data System (ADS)

    Runiu, Fang; Xin, Sun; Min, Miao; Yufeng, Jin

    2016-10-01

    In this paper, a new type of through-silicon via (TSV) for via-first process namely bare TSV, is proposed and analyzed with the aim of mitigating noise coupling problems in 3D integrated systems for advanced technology nodes. The bare TSVs have no insulation layers, and are divided into two types: bare signal TSVs and bare ground TSVs. First, by solving Poisson's equation for cylindrical P-N junctions, the bare signal TSVs are shown to be equivalent to conventional signal TSVs according to the simulation results. Then the bare ground TSV is proved to have improved noise-absorption capability when compared with a conventional ground TSV. Also, the proposed bare TSVs offer more advantages to circuits than other noise isolation methods, because the original circuit design, routing and placement can be retained after the application of the bare TSVs. Project supported by the National Basic Research Program of China (No. 2015CB0572), and the Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions (No. CIT&TCD20150320), and the National Natural Science Foundation of China (No. 61176102).

  5. Flexible fabrication of multi-scale integrated 3D periodic nanostructures with phase mask

    NASA Astrophysics Data System (ADS)

    Yuan, Liang Leon

    Top-down fabrication of artificial nanostructures, especially three-dimensional (3D) periodic nanostructures, that forms uniform and defect-free structures over large area with the advantages of high throughput and rapid processing and in a manner that can further monolithically integrate into multi-scale and multi-functional devices is long-desired but remains a considerable challenge. This thesis study advances diffractive optical element (DOE) based 3D laser holographic nanofabrication of 3D periodic nanostructures and develops new kinds of DOEs for advanced diffracted-beam control during the fabrication. Phase masks, as one particular kind of DOE, are a promising direction for simple and rapid fabrication of 3D periodic nanostructures by means of Fresnel diffraction interference lithography. When incident with a coherent beam of light, a suitable phase mask (e.g. with 2D nano-grating) can create multiple diffraction orders that are inherently phase-locked and overlap to form a 3D light interference pattern in the proximity of the DOE. This light pattern is typically recorded in photosensitive materials including photoresist to develop into 3D photonic crystal nanostructure templates. Two kinds of advanced phase masks were developed that enable delicate phase control of multiple diffraction beams. The first exploits femtosecond laser direct writing inside fused silica to assemble multiple (up to nine) orthogonally crossed (2D) grating layers, spaced on Talbot planes to overcome the inherent weak diffraction efficiency otherwise found in low-contrast volume gratings. A systematic offsetting of orthogonal grating layers to establish phase offsets over 0 to pi/2 range provided precise means for controlling the 3D photonic crystal structure symmetry between body centered tetragonal (BCT) and woodpile-like tetragonal (wTTR). The second phase mask consisted of two-layered nanogratings with small sub-wavelength grating periods and phase offset control. That was

  6. Three-Dimensional Integrated Characterization and Archiving System (3D-ICAS). Phase 1

    SciTech Connect

    1994-07-01

    3D-ICAS is being developed to support Decontamination and Decommissioning operations for DOE addressing Research Area 6 (characterization) of the Program Research and Development Announcement. 3D-ICAS provides in-situ 3-dimensional characterization of contaminated DOE facilities. Its multisensor probe contains a GC/MS (gas chromatography/mass spectrometry using noncontact infrared heating) sensor for organics, a molecular vibrational sensor for base material identification, and a radionuclide sensor for radioactive contaminants. It will provide real-time quantitative measurements of volatile organics and radionuclides on bare materials (concrete, asbestos, transite); it will provide 3-D display of the fusion of all measurements; and it will archive the measurements for regulatory documentation. It consists of two robotic mobile platforms that operate in hazardous environments linked to an integrated workstation in a safe environment.

  7. 3D Navigation and Integrated Hazard Display in Advanced Avionics: Workload, Performance, and Situation Awareness

    NASA Technical Reports Server (NTRS)

    Wickens, Christopher D.; Alexander, Amy L.

    2004-01-01

    We examined the ability for pilots to estimate traffic location in an Integrated Hazard Display, and how such estimations should be measured. Twelve pilots viewed static images of traffic scenarios and then estimated the outside world locations of queried traffic represented in one of three display types (2D coplanar, 3D exocentric, and split-screen) and in one of four conditions (display present/blank crossed with outside world present/blank). Overall, the 2D coplanar display best supported both vertical (compared to 3D) and lateral (compared to split-screen) traffic position estimation performance. Costs of the 3D display were associated with perceptual ambiguity. Costs of the split screen display were inferred to result from inappropriate attention allocation. Furthermore, although pilots were faster in estimating traffic locations when relying on memory, accuracy was greatest when the display was available.

  8. An integrated microsystem for multiplex processing of encoded silicon microbeads

    NASA Astrophysics Data System (ADS)

    Hoffmann, Daniel; Curtin, Maeve; Loughran, Michael

    2007-01-01

    A novel integrated microsystem for multiplex processing of encoded microbeads on a single microchip is presented. Conventional bio-analysis of proteins and DNA requires a combination of different techniques including: accurate delivery of reagents, mixing, then reaction at controlled temperature to yield a detectable product. Standard laboratory bio-assays require intervention at several stages to manipulate samples. Furthermore, ultra sensitive quantification with a colorimetric or fluorescent label is required to obtain the necessary results. This process is time consuming and labour intensive. However the new multiplex microsystem reported here enhances logical bio-assay development due to the integration of a compact optical detection system with customized analysis software in an enclosed microfluidic environment. The bioassay was realised by careful integration of a Peltier cell and associated control electronics which enabled specific identification of a hybridized DNA sequence from a 4 x 4 cDNA library at a fixed temperature of 42 °C. The multiple fluorescence measurements of complementary hybridised DNA at the surface of encoded microbeads was confirmed. Thus, the complete integration of the different bio-assay components on a single multiplex assay platform provides distinct advantages of reduced sample volume, rapid analysis and low cost.

  9. Femtosecond laser 3D nanofabrication in glass: enabling direct write of integrated micro/nanofluidic chips

    NASA Astrophysics Data System (ADS)

    Cheng, Ya; Liao, Yang; Sugioka, Koji

    2014-03-01

    The creation of complex three-dimensional (3D) fluidic systems composed of hollow micro- and nanostructures embedded in transparent substrates has attracted significant attention from both scientific and applied research communities. However, it is by now still a formidable challenge to build 3D micro- and nanofluidic structures with arbitrary configurations using conventional planar lithographic fabrication methods. As a direct and maskless fabrication technique, femtosecond laser micromachining provides a straightforward approach for high-precision spatial-selective modification inside transparent materials through nonlinear optical absorption. Here, we demonstrate rapid fabrication of high-aspect-ratio micro- and/or nanofluidic structures with various 3D configurations in glass substrates by femtosecond laser direct writing. Based on this approach, we demonstrate several functional micro- and nanofluidic devices including a 3D passive microfluidic mixer, a capillary electrophoresis (CE) analysis chip, and an integrated micro-nanofluidic system for single DNA analysis. This technology offers new opportunities to develop novel 3D micro-nanofluidic systems for a variety of lab-on-a-chip applications.

  10. The benefits of enhanced integration capabilities in 3-D reservoir modelling and simulation

    SciTech Connect

    O`Rourke, S.T.; Ikwumonu, A.

    1996-12-31

    The use of proprietary, closely linked 3-D geological and reservoir simulation software has greatly enhanced the reservoir modelling process by enabling complete integration of geological and engineering data in a 3-D manner. The software were used to model and simulate a deltaic sandstone reservoir in the Nigerian Forcados Yokri field in order to describe the reservoir sweep pattern. A simple simulation of the reservoir was first carried out to identify the main controls on the reservoir performance, which in this case were the intra-reservoir shales. As they are the only baffles or barriers to flow, proper modelling of them was critical to achieving a history match. Well logs, 3-D seismic, limited core data and sequence stratigraphic concepts were used to define a three dimensional depositional model which was then used to guide the 3-D reservoir architecture modelling. The reservoir model was evaluated in the 3-D simulator and, when the initial model did not yield a proper match with the historical production data, alternative models were easily generated and simulated until an acceptable match was achieved. The result was a 10% increase in predicted ultimate recovery, a better understanding of the reservoir and an optimized reservoir depletion plan.

  11. 3D shape shearography with integrated structured light projection for strain inspection of curved objects

    NASA Astrophysics Data System (ADS)

    Anisimov, Andrei G.; Groves, Roger M.

    2015-05-01

    Shearography (speckle pattern shearing interferometry) is a non-destructive testing technique that provides full-field surface strain characterization. Although real-life objects especially in aerospace, transport or cultural heritage are not flat (e.g. aircraft leading edges or sculptures), their inspection with shearography is of interest for both hidden defect detection and material characterization. Accurate strain measuring of a highly curved or free form surface needs to be performed by combining inline object shape measuring and processing of shearography data in 3D. Previous research has not provided a general solution. This research is devoted to the practical questions of 3D shape shearography system development for surface strain characterization of curved objects. The complete procedure of calibration and data processing of a 3D shape shearography system with integrated structured light projector is presented. This includes an estimation of the actual shear distance and a sensitivity matrix correction within the system field of view. For the experimental part a 3D shape shearography system prototype was developed. It employs three spatially-distributed shearing cameras, with Michelson interferometers acting as the shearing devices, one illumination laser source and a structured light projector. The developed system performance was evaluated with a previously reported cylinder specimen (length 400 mm, external diameter 190 mmm) loaded by internal pressure. Further steps for the 3D shape shearography prototype and the technique development are also proposed.

  12. A multiply-add engine with monolithically integrated 3D memristor crossbar/CMOS hybrid circuit.

    PubMed

    Chakrabarti, B; Lastras-Montaño, M A; Adam, G; Prezioso, M; Hoskins, B; Cheng, K-T; Strukov, D B

    2017-02-14

    Silicon (Si) based complementary metal-oxide semiconductor (CMOS) technology has been the driving force of the information-technology revolution. However, scaling of CMOS technology as per Moore's law has reached a serious bottleneck. Among the emerging technologies memristive devices can be promising for both memory as well as computing applications. Hybrid CMOS/memristor circuits with CMOL (CMOS + "Molecular") architecture have been proposed to combine the extremely high density of the memristive devices with the robustness of CMOS technology, leading to terabit-scale memory and extremely efficient computing paradigm. In this work, we demonstrate a hybrid 3D CMOL circuit with 2 layers of memristive crossbars monolithically integrated on a pre-fabricated CMOS substrate. The integrated crossbars can be fully operated through the underlying CMOS circuitry. The memristive devices in both layers exhibit analog switching behavior with controlled tunability and stable multi-level operation. We perform dot-product operations with the 2D and 3D memristive crossbars to demonstrate the applicability of such 3D CMOL hybrid circuits as a multiply-add engine. To the best of our knowledge this is the first demonstration of a functional 3D CMOL hybrid circuit.

  13. A multiply-add engine with monolithically integrated 3D memristor crossbar/CMOS hybrid circuit

    NASA Astrophysics Data System (ADS)

    Chakrabarti, B.; Lastras-Montaño, M. A.; Adam, G.; Prezioso, M.; Hoskins, B.; Cheng, K.-T.; Strukov, D. B.

    2017-02-01

    Silicon (Si) based complementary metal-oxide semiconductor (CMOS) technology has been the driving force of the information-technology revolution. However, scaling of CMOS technology as per Moore’s law has reached a serious bottleneck. Among the emerging technologies memristive devices can be promising for both memory as well as computing applications. Hybrid CMOS/memristor circuits with CMOL (CMOS + “Molecular”) architecture have been proposed to combine the extremely high density of the memristive devices with the robustness of CMOS technology, leading to terabit-scale memory and extremely efficient computing paradigm. In this work, we demonstrate a hybrid 3D CMOL circuit with 2 layers of memristive crossbars monolithically integrated on a pre-fabricated CMOS substrate. The integrated crossbars can be fully operated through the underlying CMOS circuitry. The memristive devices in both layers exhibit analog switching behavior with controlled tunability and stable multi-level operation. We perform dot-product operations with the 2D and 3D memristive crossbars to demonstrate the applicability of such 3D CMOL hybrid circuits as a multiply-add engine. To the best of our knowledge this is the first demonstration of a functional 3D CMOL hybrid circuit.

  14. Integrating Online and Offline 3D Deep Learning for Automated Polyp Detection in Colonoscopy Videos.

    PubMed

    Yu, Lequan; Chen, Hao; Dou, Qi; Qin, Jing; Heng, Pheng Ann

    2016-12-07

    Automated polyp detection in colonoscopy videos has been demonstrated to be a promising way for colorectal cancer (CRC) prevention and diagnosis. Traditional manual screening is time-consuming, operator-dependent and error-prone; hence, automated detection approach is highly demanded in clinical practice. However, automated polyp detection is very challenging due to high intra-class variations in polyp size, color, shape and texture and low inter-class variations between polyps and hard mimics. In this paper, we propose a novel offline and online 3D deep learning integration framework by leveraging the 3D fully convolutional network (3D-FCN) to tackle this challenging problem. Compared with previous methods employing hand-crafted features or 2D-CNNs, the 3D-FCN is capable of learning more representative spatio-temporal features from colonoscopy videos, and hence has more powerful discrimination capability. More importantly, we propose a novel online learning scheme to deal with the problem of limited training data by harnessing the specific information of an input video in the learning process. We integrate offline and online learning to effectively reduce the number of false positives generated by the offline network and further improve the detection performance. Extensive experiments on the dataset of MICCAI 2015 Challenge on Polyp Detection demonstrated the better performance of our method when compared with other competitors.

  15. A multiply-add engine with monolithically integrated 3D memristor crossbar/CMOS hybrid circuit

    PubMed Central

    Chakrabarti, B.; Lastras-Montaño, M. A.; Adam, G.; Prezioso, M.; Hoskins, B.; Cheng, K.-T.; Strukov, D. B.

    2017-01-01

    Silicon (Si) based complementary metal-oxide semiconductor (CMOS) technology has been the driving force of the information-technology revolution. However, scaling of CMOS technology as per Moore’s law has reached a serious bottleneck. Among the emerging technologies memristive devices can be promising for both memory as well as computing applications. Hybrid CMOS/memristor circuits with CMOL (CMOS + “Molecular”) architecture have been proposed to combine the extremely high density of the memristive devices with the robustness of CMOS technology, leading to terabit-scale memory and extremely efficient computing paradigm. In this work, we demonstrate a hybrid 3D CMOL circuit with 2 layers of memristive crossbars monolithically integrated on a pre-fabricated CMOS substrate. The integrated crossbars can be fully operated through the underlying CMOS circuitry. The memristive devices in both layers exhibit analog switching behavior with controlled tunability and stable multi-level operation. We perform dot-product operations with the 2D and 3D memristive crossbars to demonstrate the applicability of such 3D CMOL hybrid circuits as a multiply-add engine. To the best of our knowledge this is the first demonstration of a functional 3D CMOL hybrid circuit. PMID:28195239

  16. Integration of 3D photogrammetric outcrop models in the reservoir modelling workflow

    NASA Astrophysics Data System (ADS)

    Deschamps, Remy; Joseph, Philippe; Lerat, Olivier; Schmitz, Julien; Doligez, Brigitte; Jardin, Anne

    2014-05-01

    3D technologies are now widely used in geosciences to reconstruct outcrops in 3D. The technology used for the 3D reconstruction is usually based on Lidar, which provides very precise models. Such datasets offer the possibility to build well-constrained outcrop analogue models for reservoir study purposes. The photogrammetry is an alternate methodology which principles are based in determining the geometric properties of an object from photographic pictures taken from different angles. Outcrop data acquisition is easy, and this methodology allows constructing 3D outcrop models with many advantages such as: - light and fast acquisition, - moderate processing time (depending on the size of the area of interest), - integration of field data and 3D outcrops into the reservoir modelling tools. Whatever the method, the advantages of digital outcrop model are numerous as already highlighted by Hodgetts (2013), McCaffrey et al. (2005) and Pringle et al. (2006): collection of data from otherwise inaccessible areas, access to different angles of view, increase of the possible measurements, attributes analysis, fast rate of data collection, and of course training and communication. This paper proposes a workflow where 3D geocellular models are built by integrating all sources of information from outcrops (surface picking, sedimentological sections, structural and sedimentary dips…). The 3D geomodels that are reconstructed can be used at the reservoir scale, in order to compare the outcrop information with subsurface models: the detailed facies models of the outcrops are transferred into petrophysical and acoustic models, which are used to test different scenarios of seismic and fluid flow modelling. The detailed 3D models are also used to test new techniques of static reservoir modelling, based either on geostatistical approaches or on deterministic (process-based) simulation techniques. A modelling workflow has been designed to model reservoir geometries and properties from

  17. Transport analysis in toroidal helical plasmas using the integrated code: TASK3D

    NASA Astrophysics Data System (ADS)

    Wakasa, A.; Fukuyama, A.; Murakami, S.; Beidler, C. D.; Maassberg, H.; Yokoyama, M.; Sato, M.

    2009-11-01

    The integrated simulation code in helical plasmas, TASK3D, is being developed on the basis of an integrated modeling code for tokamak plasma, TASK. In helical systems, the neoclassical transport is one of the important issues in addition to the anomalous transport, because of strong temperature dependence of heat conductivity and an important role in determining the radial electric field. We have already constructed the neoclassical transport database in LHD, DGN/LHD. The mono-energetic diffusion coefficients are evaluated based on the Monte Carlo method by DCOM code and the mono-energetic diffusion coefficients database is constructed using a neural network technique. Also we apply GSRAKE code, which solves the ripple-averaged drift kinetic equation, to obtain transport coefficients in highly collisionless regime. We have newly incorporated the DGN/LHD module into TASK3D. We will present several results of transport simulation in typical LHD plasmas.

  18. Integrated Navigation, Guidance, and Control of Missile Systems: 3-D Dynamic Model

    DTIC Science & Technology

    2013-02-01

    UNCLASSIFIED DSTO-TR-2805 Figure B.1: Aerodynamic variables for a missile and is the lift coefficient . LC  , represent respectively the...UNCLASSIFIED Integrated Navigation, Guidance, and Control of Missile Systems: 3-D Dynamic Model Farhan A. Faruqi Weapons...engagement kinematics is derived suitable for developing, implementing and testing modern missile guidance systems. The model developed here is

  19. Proposal for the development of 3D Vertically Integrated Pattern Recognition Associative Memory (VIPRAM)

    SciTech Connect

    Deptuch, Gregory; Hoff, Jim; Kwan, Simon; Lipton, Ron; Liu, Ted; Ramberg, Erik; Todri, Aida; Yarema, Ray; Demarteua, Marcel,; Drake, Gary; Weerts, Harry; /Argonne /Chicago U. /Padua U. /INFN, Padua

    2010-10-01

    Future particle physics experiments looking for rare processes will have no choice but to address the demanding challenges of fast pattern recognition in triggering as detector hit density becomes significantly higher due to the high luminosity required to produce the rare process. The authors propose to develop a 3D Vertically Integrated Pattern Recognition Associative Memory (VIPRAM) chip for HEP applications, to advance the state-of-the-art for pattern recognition and track reconstruction for fast triggering.

  20. 3D-printed concentrators for tracking-integrated CPV modules

    NASA Astrophysics Data System (ADS)

    Apostoleris, Harry; Leland, Julian; Chiesa, Matteo; Stefancich, Marco

    2016-09-01

    We demonstrate 3D-printed nonimaging concentrators and propose a tracking integration scheme to reduce the external tracking requirements of CPV modules. In the proposed system, internal sun tracking is achieved by rotation of the mini-concentrators inside the module by small motors. We discuss the design principles employed in the development of the system, experimentally evaluate the performance of the concentrator prototypes, and propose practical modifications that may be made to improve on-site performance of the devices.

  1. Perceptual integration for qualitatively different 3-D cues in the human brain.

    PubMed

    Dövencioğlu, Dicle; Ban, Hiroshi; Schofield, Andrew J; Welchman, Andrew E

    2013-09-01

    The visual system's flexibility in estimating depth is remarkable: We readily perceive 3-D structure under diverse conditions from the seemingly random dots of a "magic eye" stereogram to the aesthetically beautiful, but obviously flat, canvasses of the Old Masters. Yet, 3-D perception is often enhanced when different cues specify the same depth. This perceptual process is understood as Bayesian inference that improves sensory estimates. Despite considerable behavioral support for this theory, insights into the cortical circuits involved are limited. Moreover, extant work tested quantitatively similar cues, reducing some of the challenges associated with integrating computationally and qualitatively different signals. Here we address this challenge by measuring fMRI responses to depth structures defined by shading, binocular disparity, and their combination. We quantified information about depth configurations (convex "bumps" vs. concave "dimples") in different visual cortical areas using pattern classification analysis. We found that fMRI responses in dorsal visual area V3B/KO were more discriminable when disparity and shading concurrently signaled depth, in line with the predictions of cue integration. Importantly, by relating fMRI and psychophysical tests of integration, we observed a close association between depth judgments and activity in this area. Finally, using a cross-cue transfer test, we found that fMRI responses evoked by one cue afford classification of responses evoked by the other. This reveals a generalized depth representation in dorsal visual cortex that combines qualitatively different information in line with 3-D perception.

  2. Multispectral photon counting integral imaging system for color visualization of photon limited 3D scenes

    NASA Astrophysics Data System (ADS)

    Moon, Inkyu

    2014-06-01

    This paper provides an overview of a colorful photon-counting integral imaging system using Bayer elemental images for 3D visualization of photon limited scenes. The color image sensor with a format of Bayer color filter array, i.e., a red, a green, or a blue filter in a repeating pattern, captures elemental image set of a photon limited three-dimensional (3D) scene. It is assumed that the observed photon count in each channel (red, green or blue) follows Poisson statistics. The reconstruction of 3D scene with a format of Bayer is obtained by applying computational geometrical ray back propagation algorithm and parametric maximum likelihood estimator to the photon-limited Bayer elemental images. Finally, several standard demosaicing algorithms are applied in order to convert the 3D reconstruction with a Bayer format into a RGB per pixel format. Experimental results demonstrate that the gradient corrected linear interpolation technique achieves better performance in regard with acceptable PSNR and less computational complexity.

  3. Silicon nanophotonic integrated devices enabling multiplexed on-chip optical interconnects

    NASA Astrophysics Data System (ADS)

    Dai, Daoxin; Wang, Jian; Chen, Sitao

    2015-05-01

    Advanced multiplexing technologies including wavelength-division-multiplexing (WDM), polarization-division multiplexing (PDM), and mode-division multiplexing (MDM) have been utilized as a cost-effective solution to enhance the capacity of an optical-interconnect link. The on-chip (de)multiplexers, including WDM filters, PDM devices, and MDM devices, are the most important key components in a multi-channel multiplexed optical interconnect system. Hybrid (de)multiplexer to enable various multiplexing technologies simultaneously are becoming more and more important to achieve many channels. In this paper we give a review for our recent work on silicon photonic integrated devices for realizing multi-channel multiplexed on-chip optical interconnects.

  4. 3D integral imaging display by smart pseudoscopic-to-orthoscopic conversion (SPOC).

    PubMed

    Navarro, H; Martínez-Cuenca, R; Saavedra, G; Martínez-Corral, M; Javidi, B

    2010-12-06

    Previously, we reported a digital technique for formation of real, non-distorted, orthoscopic integral images by direct pickup. However the technique was constrained to the case of symmetric image capture and display systems. Here, we report a more general algorithm which allows the pseudoscopic to orthoscopic transformation with full control over the display parameters so that one can generate a set of synthetic elemental images that suits the characteristics of the Integral-Imaging monitor and permits control over the depth and size of the reconstructed 3D scene.

  5. Proof of Concept of Integrated Load Measurement in 3D Printed Structures

    PubMed Central

    Hinderdael, Michaël; Jardon, Zoé; Lison, Margot; De Baere, Dieter; Devesse, Wim; Strantza, Maria; Guillaume, Patrick

    2017-01-01

    Currently, research on structural health monitoring systems is focused on direct integration of the system into a component or structure. The latter results in a so-called smart structure. One example of a smart structure is a component with integrated strain sensing for continuous load monitoring. Additive manufacturing, or 3D printing, now also enables such integration of functions inside components. As a proof-of-concept, the Fused Deposition Modeling (FDM) technique was used to integrate a strain sensing element inside polymer (ABS) tensile test samples. The strain sensing element consisted of a closed capillary filled with a fluid and connected to an externally mounted pressure sensor. The volumetric deformation of the integrated capillary resulted in pressure changes in the fluid. The obtained pressure measurements during tensile testing are reported in this paper and compared to state-of-the-art extensometer measurements. The sensitivity of the 3D printed pressure-based strain sensor is primarily a function of the compressibility of the capillary fluid. Air- and watertightness are of critical importance for the proper functioning of the 3D printed pressure-based strain sensor. Therefore, the best after-treatment procedure was selected on basis of a comparative analysis. The obtained pressure measurements are linear with respect to the extensometer readings, and the uncertainty on the strain measurement of a capillary filled with water (incompressible fluid) is ±3.1 µstrain, which is approximately three times less sensitive than conventional strain gauges (±1 µstrain), but 32 times more sensitive than the same sensor based on air (compressible fluid) (±101 µstrain). PMID:28208779

  6. Proof of Concept of Integrated Load Measurement in 3D Printed Structures.

    PubMed

    Hinderdael, Michaël; Jardon, Zoé; Lison, Margot; De Baere, Dieter; Devesse, Wim; Strantza, Maria; Guillaume, Patrick

    2017-02-09

    Currently, research on structural health monitoring systems is focused on direct integration of the system into a component or structure. The latter results in a so-called smart structure. One example of a smart structure is a component with integrated strain sensing for continuous load monitoring. Additive manufacturing, or 3D printing, now also enables such integration of functions inside components. As a proof-of-concept, the Fused Deposition Modeling (FDM) technique was used to integrate a strain sensing element inside polymer (ABS) tensile test samples. The strain sensing element consisted of a closed capillary filled with a fluid and connected to an externally mounted pressure sensor. The volumetric deformation of the integrated capillary resulted in pressure changes in the fluid. The obtained pressure measurements during tensile testing are reported in this paper and compared to state-of-the-art extensometer measurements. The sensitivity of the 3D printed pressure-based strain sensor is primarily a function of the compressibility of the capillary fluid. Air- and watertightness are of critical importance for the proper functioning of the 3D printed pressure-based strain sensor. Therefore, the best after-treatment procedure was selected on basis of a comparative analysis. The obtained pressure measurements are linear with respect to the extensometer readings, and the uncertainty on the strain measurement of a capillary filled with water (incompressible fluid) is ±3.1 µstrain, which is approximately three times less sensitive than conventional strain gauges (±1 µstrain), but 32 times more sensitive than the same sensor based on air (compressible fluid) (±101 µstrain).

  7. Polymer optical fibers integrated directly into 3D orthogonal woven composites for sensing

    NASA Astrophysics Data System (ADS)

    Hamouda, Tamer; Seyam, Abdel-Fattah M.; Peters, Kara

    2015-02-01

    This study demonstrates that standard polymer optical fibers (POF) can be directly integrated into composites from 3D orthogonal woven preforms during the weaving process and then serve as in-situ sensors to detect damage due to bending or impact loads. Different composite samples with embedded POF were fabricated of 3D orthogonal woven composites with different parameters namely number of y-/x-layers and x-yarn density. The signal of POF was not affected significantly by the preform structure. During application of resin using VARTM technique, significant drop in backscattering level was observed due to pressure caused by vacuum on the embedded POF. Measurements of POF signal while in the final composites after resin cure indicated that the backscattering level almost returned to the original level of un-embedded POF. The POF responded to application of bending and impact loads to the composite with a reduction in the backscattering level. The backscattering level almost returned back to its original level after removing the bending load until damage was present in the composite. Similar behavior occurred due to impact events. As the POF itself is used as the sensor and can be integrated throughout the composite, large sections of future 3D woven composite structures could be monitored without the need for specialized sensors or complex instrumentation.

  8. Integrating Data Clustering and Visualization for the Analysis of 3D Gene Expression Data

    SciTech Connect

    Data Analysis and Visualization and the Department of Computer Science, University of California, Davis, One Shields Avenue, Davis CA 95616, USA,; nternational Research Training Group ``Visualization of Large and Unstructured Data Sets,'' University of Kaiserslautern, Germany; Computational Research Division, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA; Genomics Division, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley CA 94720, USA; Life Sciences Division, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley CA 94720, USA,; Computer Science Division,University of California, Berkeley, CA, USA,; Computer Science Department, University of California, Irvine, CA, USA,; All authors are with the Berkeley Drosophila Transcription Network Project, Lawrence Berkeley National Laboratory,; Rubel, Oliver; Weber, Gunther H.; Huang, Min-Yu; Bethel, E. Wes; Biggin, Mark D.; Fowlkes, Charless C.; Hendriks, Cris L. Luengo; Keranen, Soile V. E.; Eisen, Michael B.; Knowles, David W.; Malik, Jitendra; Hagen, Hans; Hamann, Bernd

    2008-05-12

    The recent development of methods for extracting precise measurements of spatial gene expression patterns from three-dimensional (3D) image data opens the way for new analyses of the complex gene regulatory networks controlling animal development. We present an integrated visualization and analysis framework that supports user-guided data clustering to aid exploration of these new complex datasets. The interplay of data visualization and clustering-based data classification leads to improved visualization and enables a more detailed analysis than previously possible. We discuss (i) integration of data clustering and visualization into one framework; (ii) application of data clustering to 3D gene expression data; (iii) evaluation of the number of clusters k in the context of 3D gene expression clustering; and (iv) improvement of overall analysis quality via dedicated post-processing of clustering results based on visualization. We discuss the use of this framework to objectively define spatial pattern boundaries and temporal profiles of genes and to analyze how mRNA patterns are controlled by their regulatory transcription factors.

  9. Flow integration transform: detecting shapes in matrix-array 3D ultrasound data

    NASA Astrophysics Data System (ADS)

    Stetten, George D.; Caines, Michael; von Ramm, Olaf T.

    1995-03-01

    Matrix-array ultrasound produces real-time 3D images of the heart, by employing a square array of transducers to steer the ultrasound beam in three dimensions electronically with no moving parts. Other 3D modalities such as MR, MUGA, and CT require the use of gated studies, which combine many cardiac cycles to produce a single average cycle. Three- dimensional ultrasound eliminates this restriction, in theory permitting the continuous measurement of cardiac ventricular volume, which we call the volumetricardiogram. Towards implementing the volumetricardiogram, we have developed the flow integration transform (FIT), which operates on a 2D slice within the volumetric ultrasound data. The 3D ultrasound machine's scan converter produces a set of such slices in real time, at any desired location and orientation, to which the FIT may then be applied. Although lacking rotational or scale invariance, the FIT is designed to operate in dedicated hardware where an entire transform could be completed within a few microseconds with present integrated circuit technology. This speed would permit the application of a large battery of test shapes, or the evolution of the test shape to converge on that of the actual target.

  10. Autostereoscopic 3D Display with Long Visualization Depth Using Referential Viewing Area-Based Integral Photography.

    PubMed

    Hongen Liao; Dohi, Takeyoshi; Nomura, Keisuke

    2011-11-01

    We developed an autostereoscopic display for distant viewing of 3D computer graphics (CG) images without using special viewing glasses or tracking devices. The images are created by employing referential viewing area-based CG image generation and pixel distribution algorithm for integral photography (IP) and integral videography (IV) imaging. CG image rendering is used to generate IP/IV elemental images. The images can be viewed from each viewpoint within a referential viewing area and the elemental images are reconstructed from rendered CG images by pixel redistribution and compensation method. The elemental images are projected onto a screen that is placed at the same referential viewing distance from the lens array as in the image rendering. Photographic film is used to record the elemental images through each lens. The method enables 3D images with a long visualization depth to be viewed from relatively long distances without any apparent influence from deviated or distorted lenses in the array. We succeeded in creating an actual autostereoscopic images with an image depth of several meters in front of and behind the display that appear to have 3D even when viewed from a distance.

  11. Integration of a 3D perspective view in the navigation display: featuring pilot's mental model

    NASA Astrophysics Data System (ADS)

    Ebrecht, L.; Schmerwitz, S.

    2015-05-01

    Synthetic vision systems (SVS) appear as spreading technology in the avionic domain. Several studies prove enhanced situational awareness when using synthetic vision. Since the introduction of synthetic vision a steady change and evolution started concerning the primary flight display (PFD) and the navigation display (ND). The main improvements of the ND comprise the representation of colored ground proximity warning systems (EGPWS), weather radar, and TCAS information. Synthetic vision seems to offer high potential to further enhance cockpit display systems. Especially, concerning the current trend having a 3D perspective view in a SVS-PFD while leaving the navigational content as well as methods of interaction unchanged the question arouses if and how the gap between both displays might evolve to a serious problem. This issue becomes important in relation to the transition and combination of strategic and tactical flight guidance. Hence, pros and cons of 2D and 3D views generally as well as the gap between the egocentric perspective 3D view of the PFD and the exocentric 2D top and side view of the ND will be discussed. Further a concept for the integration of a 3D perspective view, i.e., bird's eye view, in synthetic vision ND will be presented. The combination of 2D and 3D views in the ND enables a better correlation of the ND and the PFD. Additionally, this supports the building of pilot's mental model. The authors believe it will improve the situational and spatial awareness. It might prove to further raise the safety margin when operating in mountainous areas.

  12. Uniform integration of gold nanoparticles in PDMS microfluidics with 3D micromixing

    NASA Astrophysics Data System (ADS)

    SadAbadi, H.; Packirisamy, M.; Wuthrich, R.

    2015-09-01

    The integration of gold nanoparticles (AuNPs) on the surface of polydimethylsiloxane (PDMS) microfluidics for biosensing applications is a challenging task. In this paper we address this issue by integration of pre-synthesized AuNPs (in a microreactor) into a microfluidic system. This method explored the affinity of AuNPs toward the PDMS surface so that the pre-synthesized particles will be adsorbed onto the channel walls. AuNPs were synthesized inside a microreactor before integration. In order to improve the size uniformity of the synthesized AuNPs and also to provide full mixing of reactants, a 3D-micromixer was designed, fabricated and then integrated with the microreactor in a single platform. SEM and UV/Vis spectroscopy were used to characterize the AuNPs on the PDMS surface.

  13. 3D Porosity Estimation of the Nankai Trough Sediments from Core-log-seismic Integration

    NASA Astrophysics Data System (ADS)

    Park, J. O.

    2015-12-01

    The Nankai Trough off southwest Japan is one of the best subduction-zone to study megathrust earthquake fault. Historic, great megathrust earthquakes with a recurrence interval of 100-200 yr have generated strong motion and large tsunamis along the Nankai Trough subduction zone. At the Nankai Trough margin, the Philippine Sea Plate (PSP) is being subducted beneath the Eurasian Plate to the northwest at a convergence rate ~4 cm/yr. The Shikoku Basin, the northern part of the PSP, is estimated to have opened between 25 and 15 Ma by backarc spreading of the Izu-Bonin arc. The >100-km-wide Nankai accretionary wedge, which has developed landward of the trench since the Miocene, mainly consists of offscraped and underplated materials from the trough-fill turbidites and the Shikoku Basin hemipelagic sediments. Particularly, physical properties of the incoming hemipelagic sediments may be critical for seismogenic behavior of the megathrust fault. We have carried out core-log-seismic integration (CLSI) to estimate 3D acoustic impedance and porosity for the incoming sediments in the Nankai Trough. For the CLSI, we used 3D seismic reflection data, P-wave velocity and density data obtained during IODP (Integrated Ocean Drilling Program) Expeditions 322 and 333. We computed acoustic impedance depth profiles for the IODP drilling sites from P-wave velocity and density data. We constructed seismic convolution models with the acoustic impedance profiles and a source wavelet which is extracted from the seismic data, adjusting the seismic models to observed seismic traces with inversion method. As a result, we obtained 3D acoustic impedance volume and then converted it to 3D porosity volume. In general, the 3D porosities show decrease with depth. We found a porosity anomaly zone with alteration of high and low porosities seaward of the trough axis. In this talk, we will show detailed 3D porosity of the incoming sediments, and present implications of the porosity anomaly zone for the

  14. Three-dimensional (3D) monolithically integrated photodetector and WDM receiver based on bulk silicon wafer.

    PubMed

    Song, Junfeng; Luo, Xianshu; Tu, Xiaoguang; Jia, Lianxi; Fang, Qing; Liow, Tsung-Yang; Yu, Mingbin; Lo, Guo-Qiang

    2014-08-11

    We propose a novel three-dimensional (3D) monolithic optoelectronic integration platform. Such platform integrates both electrical and photonic devices in a bulk silicon wafer, which eliminates the high-cost silicon-on-insulator (SOI) wafer and is more suitable for process requirements of electronic and photonic integrated circuits (ICs). For proof-of-concept, we demonstrate a three-dimensional photodetector and WDM receiver system. The Ge is grown on a 8-inch bulk silicon wafer while the optical waveguide is defined in a SiN layer which is deposited on top of it, with ~4 µm oxide sandwiched in between. The light is directed to the Ge photodetector from the SiN waveguide vertically by using grating coupler with a Aluminum mirror on top of it. The measured photodetector responsivity is ~0.2 A/W and the 3-dB bandwidth is ~2 GHz. Using such vertical-coupled photodetector, we demonstrated an 8-channel receiver by integrating a 1 × 8 arrayed waveguide grating (AWG). High-quality optical signal detection with up to 10 Gbit/s data rate is demonstrated, suggesting a 80 Gbit/s throughput. Such receiver can be applied to on-chip optical interconnect, DRAM interface, and telecommunication systems.

  15. 3D probe array integrated with a front-end 100-channel neural recording ASIC

    NASA Astrophysics Data System (ADS)

    Cheng, Ming-Yuan; Yao, Lei; Tan, Kwan Ling; Lim, Ruiqi; Li, Peng; Chen, Weiguo

    2014-12-01

    Brain-machine interface technology can improve the lives of spinal cord injury victims and amputees. A neural interface system, consisting of a 3D probe array and a custom low-power (1 mW) 100-channel (100-ch) neural recording application-specific integrated circuit (ASIC), was designed and implemented to monitor neural activity. In this study, a microassembly 3D probe array method using a novel lead transfer technique was proposed to overcome the bonding plane mismatch encountered during orthogonal assembly. The proposed lead transfer technique can be completed using standard micromachining and packaging processes. The ASIC can be stacking-integrated with the probe array, minimizing the form factor of the assembled module. To minimize trauma to brain cells, the profile of the integrated probe array was controlled within 730 μm. The average impedance of the assembled probe was approximately 0.55 MΩ at 1 kHz. To verify the functionality of the integrated neural probe array, bench-top signal acquisitions were performed and discussed.

  16. Catch and release: integrated system for multiplexed detection of bacteria.

    PubMed

    Verbarg, Jasenka; Plath, William D; Shriver-Lake, Lisa C; Howell, Peter B; Erickson, Jeffrey S; Golden, Joel P; Ligler, Frances S

    2013-05-21

    An integrated system with automated immunomagnetic separation and processing of fluidic samples was demonstrated for multiplexed optical detection of bacterial targets. Mixtures of target-specific magnetic bead sets were processed in the NRL MagTrap with the aid of rotating magnet arrays that entrapped and moved the beads within the channel during reagent processing. Processing was performed in buffer and human serum matrixes with 10-fold dilutions in the range of 10(2)-10(6) cells/mL of target bacteria. Reversal of magnets' rotation post-processing released the beads back into the flow and moved them into the microflow cytometer for optical interrogation. Identification of the beads and the detection of PE fluorescence were performed simultaneously for multiplexed detection. Multiplexing was performed with specifically targeted bead sets to detect E. coli 0157.H7, Salmonella Common Structural Antigen, Listeria sp., and Shigella sp., dose-response curves were obtained, and limits of detection were calculated for each target in the buffer and clinical matrix. Additional tests demonstrated the potential for using the MagTrap to concentrate target from larger volumes of sample prior to the addition of assay reagents.

  17. An integrated lubricant oil conditioning sensor using signal multiplexing

    NASA Astrophysics Data System (ADS)

    Zhu, Xiaoliang; Du, Li; Zhe, Jiang

    2015-01-01

    One effective approach to detect signs of potential failure of a rotating or reciprocating machine is to examine the conditions of its lubrication oil. Here we present an integrated oil condition sensor for detecting both wear debris and lubricant properties. The integrated sensor consists of miniature multiplexed sensing elements for detection of wear debris and measurements of viscosity and moisture. The oil debris sensing element consists of eight sensing channels to detect wear debris in parallel; the elements for measuring oil viscosity and moisture, based on interdigital electrode sensing, were fabricated using micromachining. The integrated sensor was installed and tested in a laboratory lubricating system. Signal multiplexing was applied to the outputs of the three sensing elements such that responses from all sensing elements were obtained within two measurements, and the signal-to-noise ratio was improved. Testing results show that the integrated sensor is capable of measuring wear debris (>50 µm), moisture (>50 ppm) and viscosity (>12.4 cSt) at a high throughput (200 ml min-1). The device can be potentially used for online health monitoring of rotating machines.

  18. 3D modeling of a dolerite intrusion from the photogrammetric and geophysical data integration.

    NASA Astrophysics Data System (ADS)

    Duarte, João; Machadinho, Ana; Figueiredo, Fernando; Mira, Maria

    2015-04-01

    The aims of this study is create a methodology based on the integration of data obtained from various available technologies, which allow a credible and complete evaluation of rock masses. In this particular case of a dolerite intrusion, which deployed an exploration of aggregates and belongs to the Jobasaltos - Extracção e Britagem. S.A.. Dolerite intrusion is situated in the volcanic complex of Serra de Todo-o-Mundo, Casais Gaiola, intruded in Jurassic sandstones. The integration of the surface and subsurface mapping, obtained by technology UAVs (Drone) and geophysical surveys (Electromagnetic Method - TEM 48 FAST), allows the construction of 2D and 3D models of the study local. The combination of the 3D point clouds produced from two distinct processes, modeling of photogrammetric and geophysical data, will be the basis for the construction of a single model of set. The rock masses in an integral perspective being visible their development above the surface and subsurface. The presentation of 2D and 3D models will give a perspective of structures, fracturation, lithology and their spatial correlations contributing to a better local knowledge, as well as its potential for the intended purpose. From these local models it will be possible to characterize and quantify the geological structures. These models will have its importance as a tool to assist in the analysis and drafting of regional models. The qualitative improvement in geological/structural modeling, seeks to reduce the value of characterization/cost ratio, in phase of prospecting, improving the investment/benefit ratio. This methodology helps to assess more accurately the economic viability of the projects.

  19. Intracranial Catheter for Integrated 3D Ultrasound Imaging & Hyperthermia: Feasibility Study

    NASA Astrophysics Data System (ADS)

    Herickhoff, Carl D.; Light, Edward D.; Bing, Kristin Frinkley; Mukundan, Srinivasan; Grant, Gerald A.; Wolf, Patrick D.; Dixon-Tulloch, Ellen; Shih, Timothy; Hsu, Stephen J.; Smith, Stephen W.

    2009-04-01

    In this study, we investigated the feasibility of an intracranial catheter transducer capable of real-time 3D (RT3D) imaging and ultrasound hyperthermia, for application in the visualization and treatment of tumors in the brain. We designed and constructed a 12 Fr, integrated matrix and linear array catheter transducer prototype for combined RT3D imaging and heating capability. This dual-mode catheter incorporated 153 matrix array elements and 11 linear array elements, on a 0.2 mm pitch, with a total aperture size of 8.4 mm×2.3 mm. This array achieved a 3.5° C in vitro temperature rise at a 2 cm focal distance in tissue-mimicking material. The dual-mode catheter prototype was compared with a Siemens 10 Fr AcuNav™ catheter as a gold standard in experiments assessing image quality and therapeutic potential, and both probes were used in a canine brain model to image anatomical structures and color Doppler blood flow and to attempt in vivo heating.

  20. Application of CART3D to Complex Propulsion-Airframe Integration with Vehicle Sketch Pad

    NASA Technical Reports Server (NTRS)

    Hahn, Andrew S.

    2012-01-01

    Vehicle Sketch Pad (VSP) is an easy-to-use modeler used to generate aircraft geometries for use in conceptual design and analysis. It has been used in the past to generate metageometries for aerodynamic analyses ranging from handbook methods to Navier-Stokes computational fluid dynamics (CFD). As desirable as it is to bring high order analyses, such as CFD, into the conceptual design process, this has been difficult and time consuming in practice due to the manual nature of both surface and volume grid generation. Over the last couple of years, VSP has had a major upgrade of its surface triangulation and export capability. This has enhanced its ability to work with Cart3D, an inviscid, three dimensional fluid flow toolset. The combination of VSP and Cart3D allows performing inviscid CFD on complex geometries with relatively high productivity. This paper will illustrate the use of VSP with Cart3D through an example case of a complex propulsion-airframe integration (PAI) of an over-wing nacelle (OWN) airliner configuration.

  1. Integration of 3D structure from disparity into biological motion perception independent of depth awareness.

    PubMed

    Wang, Ying; Jiang, Yi

    2014-01-01

    Images projected onto the retinas of our two eyes come from slightly different directions in the real world, constituting binocular disparity that serves as an important source for depth perception - the ability to see the world in three dimensions. It remains unclear whether the integration of disparity cues into visual perception depends on the conscious representation of stereoscopic depth. Here we report evidence that, even without inducing discernible perceptual representations, the disparity-defined depth information could still modulate the visual processing of 3D objects in depth-irrelevant aspects. Specifically, observers who could not discriminate disparity-defined in-depth facing orientations of biological motions (i.e., approaching vs. receding) due to an excessive perceptual bias nevertheless exhibited a robust perceptual asymmetry in response to the indistinguishable facing orientations, similar to those who could consciously discriminate such 3D information. These results clearly demonstrate that the visual processing of biological motion engages the disparity cues independent of observers' depth awareness. The extraction and utilization of binocular depth signals thus can be dissociable from the conscious representation of 3D structure in high-level visual perception.

  2. A 3D integral imaging optical see-through head-mounted display.

    PubMed

    Hua, Hong; Javidi, Bahram

    2014-06-02

    An optical see-through head-mounted display (OST-HMD), which enables optical superposition of digital information onto the direct view of the physical world and maintains see-through vision to the real world, is a vital component in an augmented reality (AR) system. A key limitation of the state-of-the-art OST-HMD technology is the well-known accommodation-convergence mismatch problem caused by the fact that the image source in most of the existing AR displays is a 2D flat surface located at a fixed distance from the eye. In this paper, we present an innovative approach to OST-HMD designs by combining the recent advancement of freeform optical technology and microscopic integral imaging (micro-InI) method. A micro-InI unit creates a 3D image source for HMD viewing optics, instead of a typical 2D display surface, by reconstructing a miniature 3D scene from a large number of perspective images of the scene. By taking advantage of the emerging freeform optical technology, our approach will result in compact, lightweight, goggle-style AR display that is potentially less vulnerable to the accommodation-convergence discrepancy problem and visual fatigue. A proof-of-concept prototype system is demonstrated, which offers a goggle-like compact form factor, non-obstructive see-through field of view, and true 3D virtual display.

  3. Integration of regional to outcrop digital data: 3D visualisation of multi-scale geological models

    NASA Astrophysics Data System (ADS)

    Jones, R. R.; McCaffrey, K. J. W.; Clegg, P.; Wilson, R. W.; Holliman, N. S.; Holdsworth, R. E.; Imber, J.; Waggott, S.

    2009-01-01

    Multi-scale geological models contain three-dimensional, spatially referenced data, typically spanning at least six orders of magnitude from outcrop to regional scale. A large number of different geological and geophysical data sources can be combined into a single model. Established 3D visualisation methods that are widely used in hydrocarbon exploration and production for sub-surface data have been adapted for onshore surface geology through a combination of methods for digital data acquisition, 3D visualisation, and geospatial analysis. The integration of georeferenced data across a wider than normal range in scale helps to address several of the existing limitations that are inherent in traditional methods of map production and publishing. The primary advantage of a multi-scale approach is that spatial precision and dimensionality (which are generally degraded when data are displayed in 2D at a single scale) can be preserved at all scales. Real-time, immersive, interactive software, based on a "3D geospatial" graphical user interface (GUI), allows complex geological architectures to be depicted, and is more inherently intuitive than software based on a standard "desktop" GUI metaphor. The continuing convergence of different kinds of geo-modelling, GIS, and visualisation software, as well as industry acceptance of standardised middleware, has helped to make multi-scale geological models a practical reality. This is illustrated with two case studies from NE England and NW Scotland.

  4. Fully analytical integration over the 3D volume bounded by the β sphere in topological atoms.

    PubMed

    Popelier, Paul L A

    2011-11-17

    Atomic properties of a topological atom are obtained by 3D integration over the volume of its atomic basin. Algorithms that compute atomic properties typically integrate over two subspaces: the volume bounded by the so-called β sphere, which is centered at the nucleus and completely contained within the atomic basin, and the volume of the remaining part of the basin. Here we show how the usual quadrature over the β sphere volume can be replaced by a fully analytical 3D integration leading to the atomic charge (monopole moment) for s, p, and d functions. Spherical tensor multipole moments have also been implemented and tested up to hexadecupole for s functions only, and up to quadrupole for s and p functions. The new algorithm is illustrated by operating on capped glycine (HF/6-31G, 35 molecular orbitals (MOs), 322 Gaussian primitives, 19 nuclei), the protein crambin (HF/3-21G, 1260 MOs, 5922 primitives and 642 nuclei), and tin (Z = 50) in Sn(2)(CH(3))(2) (B3LYP/cc-pVTZ and LANL2DZ, 59 MOs, 1352 primitives).

  5. Design, modeling and testing of integrated ring extractor for high resolution electrohydrodynamic (EHD) 3D printing

    NASA Astrophysics Data System (ADS)

    Han, Yiwei; Dong, Jingyan

    2017-03-01

    This paper presents an integrated ring extractor design in electrohydrodynamic (EHD) printing, which can overcome the standoff height limitation in the EHD printing process, and improve printing capability for 3D structures. Standoff height in the EHD printing will affect printing processes and limit the height of the printed structure when the ground electrode is placed under the substrate. In this work, we designed and integrated a ring electrode with the printing nozzle to achieve a self-working printer head, which can start and maintain the printing process without the involvement of the substrate. We applied a FEA method to model the electric field potential distribution and strength to direct the ring extractor design, which provides a similar printing capability with the system using substrate as the ground electrode. We verified the ring electrode design by experiments, and those results from the experiments demonstrated a good match with results from the FEA simulation. We have characterized the printing processes using the integrated ring extractor, and successfully applied this newly designed ring extractor to print polycaprolactone (PCL) 3D structures.

  6. Efficient data exchange: Integrating a vector GIS with an object-oriented, 3-D visualization system

    SciTech Connect

    Kuiper, J.; Ayers, A.; Johnson, R.; Tolbert-Smith, M.

    1996-03-01

    A common problem encountered in Geographic Information System (GIS) modeling is the exchange of data between different software packages to best utilize the unique features of each package. This paper describes a project to integrate two systems through efficient data exchange. The first is a widely used GIS based on a relational data model. This system has a broad set of data input, processing, and output capabilities, but lacks three-dimensional (3-D) visualization and certain modeling functions. The second system is a specialized object-oriented package designed for 3-D visualization and modeling. Although this second system is useful for subsurface modeling and hazardous waste site characterization, it does not provide many of the, capabilities of a complete GIS. The system-integration project resulted in an easy-to-use program to transfer information between the systems, making many of the more complex conversion issues transparent to the user. The strengths of both systems are accessible, allowing the scientist more time to focus on analysis. This paper details the capabilities of the two systems, explains the technical issues associated with data exchange and how they were solved, and outlines an example analysis project that used the integrated systems.

  7. New Dimensions in Microarchitecture Harnessing 3D Integration Technologies (BRIEFING CHARTS)

    DTIC Science & Technology

    2007-03-06

    New Dimensions in Microarchitecture Harnessing 3D Integration Technologies Kerry Bernstein IBM T.J. Watson Research Center Yorktown Heights, NY...e m o r y H i e r a r c h y L1 Size limited by Cycle time Components of Processor Performance From ISCA ’06 Keynote address by Phil Emma , IBM Delay...From ISCA ’06 Keynote address by Phil Emma , IBM 6 March, 2007 New Dimensions in Microarchitecture 8 What Is Bandwidth Used For? Miss Penalty

  8. 3D printed LED based on-capillary detector housing with integrated slit.

    PubMed

    Cecil, Farhan; Zhang, Min; Guijt, Rosanne M; Henderson, Alan; Nesterenko, Pavel N; Paull, Brett; Breadmore, Michael C; Macka, Mirek

    2017-05-01

    A 3D printed photometric detector body with integrated slit was fabricated to position a LED and photodiode either side of capillary tubing using a fused deposition modelling (FDM) printer. To make this approach suitable for capillaries down to 50 μm i.d. the dimension of the in-built slit is the critical element of the printed housing. The spatial orientation of the model for printing was found to significantly impact on the resolution of the structures and voids that can be printed. By designing a housing with a slit positioned in the XY plane in parallel with the print direction, the narrowest void (slit) that could be printed was 70 μm. The potential use of the 3D printed slit for photometric detection was characterised using tubing and capillary from 500 down to 50 μm i.d, demonstrating a linear response from 632 to 40 mAU. The effective pathlength and stray light varied from 383 to 22 μm and 3.8% - 50% for 500- 50 μm i.d tubing and capillary. The use of a V-shaped alignment feature allowed for easy and reliable positioning of the tubing inside the detector, as demonstrated by a RSD of 1.9% (n = 10) in peak height when repositioning the tubing between measurements using flow injection analysis (FIA). The performance of the 3D printed housing and 70 μm slit was benchmarked against a commercially available interface using the CE separation of Zn(2+) and Cu(2+) complexes with PAR. The limit of detection with the 3D printed slit was 6.8 and 4.5 μM and is 2.8 and 1.6 μM with the commercial interface.

  9. DYNA3D, INGRID, and TAURUS: an integrated, interactive software system for crashworthiness engineering

    SciTech Connect

    Benson, D.J.; Hallquist, J.O.; Stillman, D.W.

    1985-04-01

    Crashworthiness engineering has always been a high priority at Lawrence Livermore National Laboratory because of its role in the safe transport of radioactive material for the nuclear power industry and military. As a result, the authors have developed an integrated, interactive set of finite element programs for crashworthiness analysis. The heart of the system is DYNA3D, an explicit, fully vectorized, large deformation structural dynamics code. DYNA3D has the following four capabilities that are critical for the efficient and accurate analysis of crashes: (1) fully nonlinear solid, shell, and beam elements for representing a structure, (2) a broad range of constitutive models for representing the materials, (3) sophisticated contact algorithms for the impact interactions, and (4) a rigid body capability to represent the bodies away from the impact zones at a greatly reduced cost without sacrificing any accuracy in the momentum calculations. To generate the large and complex data files for DYNA3D, INGRID, a general purpose mesh generator, is used. It runs on everything from IBM PCs to CRAYS, and can generate 1000 nodes/minute on a PC. With its efficient hidden line algorithms and many options for specifying geometry, INGRID also doubles as a geometric modeller. TAURUS, an interactive post processor, is used to display DYNA3D output. In addition to the standard monochrome hidden line display, time history plotting, and contouring, TAURUS generates interactive color displays on 8 color video screens by plotting color bands superimposed on the mesh which indicate the value of the state variables. For higher quality color output, graphic output files may be sent to the DICOMED film recorders. We have found that color is every bit as important as hidden line removal in aiding the analyst in understanding his results. In this paper the basic methodologies of the programs are presented along with several crashworthiness calculations.

  10. The OpenEarth Framework (OEF) for the 3D Visualization of Integrated Earth Science Data

    NASA Astrophysics Data System (ADS)

    Nadeau, David; Moreland, John; Baru, Chaitan; Crosby, Chris

    2010-05-01

    Data integration is increasingly important as we strive to combine data from disparate sources and assemble better models of the complex processes operating at the Earth's surface and within its interior. These data are often large, multi-dimensional, and subject to differing conventions for data structures, file formats, coordinate spaces, and units of measure. When visualized, these data require differing, and sometimes conflicting, conventions for visual representations, dimensionality, symbology, and interaction. All of this makes the visualization of integrated Earth science data particularly difficult. The OpenEarth Framework (OEF) is an open-source data integration and visualization suite of applications and libraries being developed by the GEON project at the University of California, San Diego, USA. Funded by the NSF, the project is leveraging virtual globe technology from NASA's WorldWind to create interactive 3D visualization tools that combine and layer data from a wide variety of sources to create a holistic view of features at, above, and beneath the Earth's surface. The OEF architecture is open, cross-platform, modular, and based upon Java. The OEF's modular approach to software architecture yields an array of mix-and-match software components for assembling custom applications. Available modules support file format handling, web service communications, data management, user interaction, and 3D visualization. File parsers handle a variety of formal and de facto standard file formats used in the field. Each one imports data into a general-purpose common data model supporting multidimensional regular and irregular grids, topography, feature geometry, and more. Data within these data models may be manipulated, combined, reprojected, and visualized. The OEF's visualization features support a variety of conventional and new visualization techniques for looking at topography, tomography, point clouds, imagery, maps, and feature geometry. 3D data such as

  11. On Integral Invariants for Effective 3-D Motion Trajectory Matching and Recognition.

    PubMed

    Shao, Zhanpeng; Li, Youfu

    2016-02-01

    Motion trajectories tracked from the motions of human, robots, and moving objects can provide an important clue for motion analysis, classification, and recognition. This paper defines some new integral invariants for a 3-D motion trajectory. Based on two typical kernel functions, we design two integral invariants, the distance and area integral invariants. The area integral invariants are estimated based on the blurred segment of noisy discrete curve to avoid the computation of high-order derivatives. Such integral invariants for a motion trajectory enjoy some desirable properties, such as computational locality, uniqueness of representation, and noise insensitivity. Moreover, our formulation allows the analysis of motion trajectories at a range of scales by varying the scale of kernel function. The features of motion trajectories can thus be perceived at multiscale levels in a coarse-to-fine manner. Finally, we define a distance function to measure the trajectory similarity to find similar trajectories. Through the experiments, we examine the robustness and effectiveness of the proposed integral invariants and find that they can capture the motion cues in trajectory matching and sign recognition satisfactorily.

  12. Integrated Modeling of Time Evolving 3D Kinetic MHD Equilibria and NTV Torque

    NASA Astrophysics Data System (ADS)

    Logan, N. C.; Park, J.-K.; Grierson, B. A.; Haskey, S. R.; Nazikian, R.; Cui, L.; Smith, S. P.; Meneghini, O.

    2016-10-01

    New analysis tools and integrated modeling of plasma dynamics developed in the OMFIT framework are used to study kinetic MHD equilibria evolution on the transport time scale. The experimentally observed profile dynamics following the application of 3D error fields are described using a new OMFITprofiles workflow that directly addresses the need for rapid and comprehensive analysis of dynamic equilibria for next-step theory validation. The workflow treats all diagnostic data as fundamentally time dependent, provides physics-based manipulations such as ELM phase data selection, and is consistent across multiple machines - including DIII-D and NSTX-U. The seamless integration of tokamak data and simulation is demonstrated by using the self-consistent kinetic EFIT equilibria and profiles as input into 2D particle, momentum and energy transport calculations using TRANSP as well as 3D kinetic MHD equilibrium stability and neoclassical transport modeling using General Perturbed Equilibrium Code (GPEC). The result is a smooth kinetic stability and NTV torque evolution over transport time scales. Work supported by DE-AC02-09CH11466.

  13. Shear Behavior of 3D Woven Hollow Integrated Sandwich Composites: Experimental, Theoretical and Numerical Study

    NASA Astrophysics Data System (ADS)

    Zhou, Guangming; Liu, Chang; Cai, Deng'an; Li, Wenlong; Wang, Xiaopei

    2016-11-01

    An experimental, theoretical and numerical investigation on the shear behavior of 3D woven hollow integrated sandwich composites was presented in this paper. The microstructure of the composites was studied, then the shear modulus and load-deflection curves were obtained by double lap shear tests on the specimens in two principal directions of the sandwich panels, called warp and weft. The experimental results showed that the shear modulus of the warp was higher than that of the weft and the failure occurred in the roots of piles. A finite element model was established to predict the shear behavior of the composites. The simulated results agreed well with the experimental data. Simultaneously, a theoretical method was developed to predict the shear modulus. By comparing with the experimental data, the accuracy of the theoretical method was verified. The influence of structural parameters on shear modulus was also discussed. The higher yarn number, yarn density and dip angle of the piles could all improve the shear modulus of 3D woven hollow integrated sandwich composites at different levels, while the increasing height would decrease the shear modulus.

  14. Integrating 3D seismic curvature and curvature gradient attributes for fracture characterization: Methodologies and interpretational implications

    SciTech Connect

    Gao, Dengliang

    2013-03-01

    In 3D seismic interpretation, curvature is a popular attribute that depicts the geometry of seismic reflectors and has been widely used to detect faults in the subsurface; however, it provides only part of the solutions to subsurface structure analysis. This study extends the curvature algorithm to a new curvature gradient algorithm, and integrates both algorithms for fracture detection using a 3D seismic test data set over Teapot Dome (Wyoming). In fractured reservoirs at Teapot Dome known to be formed by tectonic folding and faulting, curvature helps define the crestal portion of the reservoirs that is associated with strong seismic amplitude and high oil productivity. In contrast, curvature gradient helps better define the regional northwest-trending and the cross-regional northeast-trending lineaments that are associated with weak seismic amplitude and low oil productivity. In concert with previous reports from image logs, cores, and outcrops, the current study based on an integrated seismic curvature and curvature gradient analysis suggests that curvature might help define areas of enhanced potential to form tensile fractures, whereas curvature gradient might help define zones of enhanced potential to develop shear fractures. In certain fractured reservoirs such as at Teapot Dome where faulting and fault-related folding contribute dominantly to the formation and evolution of fractures, curvature and curvature gradient attributes can be potentially applied to differentiate fracture mode, to predict fracture intensity and orientation, to detect fracture volume and connectivity, and to model fracture networks.

  15. CISUS: an integrated 3D ultrasound system for IGT using a modular tracking API

    NASA Astrophysics Data System (ADS)

    Boctor, Emad M.; Viswanathan, Anand; Pieper, Steve; Choti, Michael A.; Taylor, Russell H.; Kikinis, Ron; Fichtinger, Gabor

    2004-05-01

    Ultrasound has become popular in clinical/surgical applications, both as the primary image guidance modality and also in conjunction with other modalities like CT or MRI. Three dimensional ultrasound (3DUS) systems have also demonstrated usefulness in image-guided therapy (IGT). At the same time, however, current lack of open-source and open-architecture multi-modal medical visualization systems prevents 3DUS from fulfilling its potential. Several stand-alone 3DUS systems, like Stradx or In-Vivo exist today. Although these systems have been found to be useful in real clinical setting, it is difficult to augment their functionality and integrate them in versatile IGT systems. To address these limitations, a robotic/freehand 3DUS open environment (CISUS) is being integrated into the 3D Slicer, an open-source research tool developed for medical image analysis and surgical planning. In addition, the system capitalizes on generic application programming interfaces (APIs) for tracking devices and robotic control. The resulting platform-independent open-source system may serve as a valuable tool to the image guided surgery community. Other researchers could straightforwardly integrate the generic CISUS system along with other functionalities (i.e. dual view visualization, registration, real-time tracking, segmentation, etc) to rapidly create their medical/surgical applications. Our current driving clinical application is robotically assisted and freehand 3DUS-guided liver ablation, which is fully being integrated under the CISUS-3D Slicer. Initial functionality and pre-clinical feasibility are demonstrated on phantom and ex-vivo animal models.

  16. Illustrating Surface Shape in Volume Data via Principal Direction-Driven 3D Line Integral Convolution

    NASA Technical Reports Server (NTRS)

    Interrante, Victoria

    1997-01-01

    The three-dimensional shape and relative depth of a smoothly curving layered transparent surface may be communicated particularly effectively when the surface is artistically enhanced with sparsely distributed opaque detail. This paper describes how the set of principal directions and principal curvatures specified by local geometric operators can be understood to define a natural 'flow' over the surface of an object, and can be used to guide the placement of the lines of a stroke texture that seeks to represent 3D shape information in a perceptually intuitive way. The driving application for this work is the visualization of layered isovalue surfaces in volume data, where the particular identity of an individual surface is not generally known a priori and observers will typically wish to view a variety of different level surfaces from the same distribution, superimposed over underlying opaque structures. By advecting an evenly distributed set of tiny opaque particles, and the empty space between them, via 3D line integral convolution through the vector field defined by the principal directions and principal curvatures of the level surfaces passing through each gridpoint of a 3D volume, it is possible to generate a single scan-converted solid stroke texture that may intuitively represent the essential shape information of any level surface in the volume. To generate longer strokes over more highly curved areas, where the directional information is both most stable and most relevant, and to simultaneously downplay the visual impact of directional information in the flatter regions, one may dynamically redefine the length of the filter kernel according to the magnitude of the maximum principal curvature of the level surface at the point around which it is applied.

  17. Scientific Subsurface data for EPOS - integration of 3D and 4D data services

    NASA Astrophysics Data System (ADS)

    Kerschke, Dorit; Hammitzsch, Martin; Wächter, Joachim

    2016-04-01

    The provision of efficient and easy access to scientific subsurface data sets obtained from field studies and scientific observatories or by geological 3D/4D-modeling is an important contribution to modern research infrastructures as they can facilitate the integrated analysis and evaluation as well as the exchange of scientific data. Within the project EPOS - European Plate Observing System, access to 3D and 4D data sets will be provided by 'WP15 - Geological information and modeling' and include structural geology models as well as numerical models, e.g., temperature, aquifers, and velocity. This also includes validated raw data, e.g., seismic profiles, from which the models where derived. All these datasets are of high quality and of unique scientific value as the process of modeling is time and cost intensive. However, these models are currently not easily accessible for the wider scientific community, much less to the public. For the provision of these data sets a data management platform based on common and standardized data models, protocols, and encodings as well as on a predominant use of Free and Open Source Software (FOSS) has been devised. The interoperability for disciplinary and domain applications thus highly depends on the adoption of generally agreed technologies and standards (OGC, ISO…) originating from Spatial Data Infrastructure related efforts (e.g., INSPIRE). However, since not many standards for 3D and 4D geological data exists, this work also includes new approaches for project data management, interfaces for tools used by the researchers, and interfaces for the sharing and reusing of data.

  18. 3D tract-specific local and global analysis of white matter integrity in Alzheimer's disease.

    PubMed

    Jin, Yan; Huang, Chao; Daianu, Madelaine; Zhan, Liang; Dennis, Emily L; Reid, Robert I; Jack, Clifford R; Zhu, Hongtu; Thompson, Paul M

    2017-03-01

    Alzheimer's disease (AD) is a chronic neurodegenerative disease characterized by progressive decline in memory and other aspects of cognitive function. Diffusion-weighted imaging (DWI) offers a non-invasive approach to delineate the effects of AD on white matter (WM) integrity. Previous studies calculated either some summary statistics over regions of interest (ROI analysis) or some statistics along mean skeleton lines (Tract Based Spatial Statistic [TBSS]), so they cannot quantify subtle local WM alterations along major tracts. Here, a comprehensive WM analysis framework to map disease effects on 3D tracts both locally and globally, based on a study of 200 subjects: 49 healthy elderly normal controls, 110 with mild cognitive impairment, and 41 AD patients has been presented. 18 major WM tracts were extracted with our automated clustering algorithm-autoMATE (automated Multi-Atlas Tract Extraction); we then extracted multiple DWI-derived parameters of WM integrity along the WM tracts across all subjects. A novel statistical functional analysis method-FADTTS (Functional Analysis for Diffusion Tensor Tract Statistics) was applied to quantify degenerative patterns along WM tracts across different stages of AD. Gradually increasing WM alterations were found in all tracts in successive stages of AD. Among all 18 WM tracts, the fornix was most adversely affected. Among all the parameters, mean diffusivity (MD) was the most sensitive to WM alterations in AD. This study provides a systematic workflow to examine WM integrity across automatically computed 3D tracts in AD and may be useful in studying other neurological and psychiatric disorders. Hum Brain Mapp 38:1191-1207, 2017. © 2016 Wiley Periodicals, Inc.

  19. 3D tract‐specific local and global analysis of white matter integrity in Alzheimer's disease

    PubMed Central

    Jin, Yan; Huang, Chao; Daianu, Madelaine; Zhan, Liang; Dennis, Emily L.; Reid, Robert I.; Jack, Clifford R.; Zhu, Hongtu

    2016-01-01

    Abstract Alzheimer's disease (AD) is a chronic neurodegenerative disease characterized by progressive decline in memory and other aspects of cognitive function. Diffusion‐weighted imaging (DWI) offers a non‐invasive approach to delineate the effects of AD on white matter (WM) integrity. Previous studies calculated either some summary statistics over regions of interest (ROI analysis) or some statistics along mean skeleton lines (Tract Based Spatial Statistic [TBSS]), so they cannot quantify subtle local WM alterations along major tracts. Here, a comprehensive WM analysis framework to map disease effects on 3D tracts both locally and globally, based on a study of 200 subjects: 49 healthy elderly normal controls, 110 with mild cognitive impairment, and 41 AD patients has been presented. 18 major WM tracts were extracted with our automated clustering algorithm—autoMATE (automated Multi‐Atlas Tract Extraction); we then extracted multiple DWI‐derived parameters of WM integrity along the WM tracts across all subjects. A novel statistical functional analysis method—FADTTS (Functional Analysis for Diffusion Tensor Tract Statistics) was applied to quantify degenerative patterns along WM tracts across different stages of AD. Gradually increasing WM alterations were found in all tracts in successive stages of AD. Among all 18 WM tracts, the fornix was most adversely affected. Among all the parameters, mean diffusivity (MD) was the most sensitive to WM alterations in AD. This study provides a systematic workflow to examine WM integrity across automatically computed 3D tracts in AD and may be useful in studying other neurological and psychiatric disorders. Hum Brain Mapp 38:1191–1207, 2017. © 2016 Wiley Periodicals, Inc. PMID:27883250

  20. The Avignon Bridge: a 3d Reconstruction Project Integrating Archaeological, Historical and Gemorphological Issues

    NASA Astrophysics Data System (ADS)

    Berthelot, M.; Nony, N.; Gugi, L.; Bishop, A.; De Luca, L.

    2015-02-01

    The history and identity of the Avignon's bridge is inseparable from that of the Rhône river. Therefore, in order to share the history and memory of the Rhône, it is essential to get to know this bridge and especially to identify and make visible the traces of its past, its construction, its interaction with the river dynamics, which greatly influenced his life. These are the objectives of the PAVAGE project that focuses on digitally surveying, modelling and re-visiting a heritage site of primary importance with the aim of virtually restoring the link between the two sides which, after the disappearance of the Roman bridge of Arles, constituted for a long time the only connection between Lyon or Vienna and the sea. Therefore, this project has an important geo-historical dimension for which geo-morphological and paleoenvironmental studies were implemented in connection with the latest digital simulation methods exploiting geographic information systems. By integrating knowledge and reflections of archaeologists, historians, geomorphologists, environmentalists, architects, engineers and computer scientists, the result of this project (which involved 5 laboratories during 4 years) is a 3D digital model covering an extension of 50 km2 achieved by integrating satellite imagery, UAV-based acquisitions, terrestrial laser scanning and photogrammetry, etc. Beyond the actions of scientific valorisation concerning the historical and geomorphological dimensions of the project, the results of this work of this interdisciplinary investigation and interpretation of this site are today integrated within a location-based augmented reality application allowing tourists to exploring the virtual reconstruction of the bridge and its environment through tablets inside the portion of territory covered by this project (between Avignon and Villeneuve-lez-Avignon). This paper presents the main aspects of the 3D virtual reconstruction approach.

  1. First Steps Towards AN Integrated Citygml-Based 3d Model of Vienna

    NASA Astrophysics Data System (ADS)

    Agugiaro, G.

    2016-06-01

    This paper presents and discusses the results regarding the initial steps (selection, analysis, preparation and eventual integration of a number of datasets) for the creation of an integrated, semantic, three-dimensional, and CityGML-based virtual model of the city of Vienna. CityGML is an international standard conceived specifically as information and data model for semantic city models at urban and territorial scale. It is being adopted by more and more cities all over the world. The work described in this paper is embedded within the European Marie-Curie ITN project "Ci-nergy, Smart cities with sustainable energy systems", which aims, among the rest, at developing urban decision making and operational optimisation software tools to minimise non-renewable energy use in cities. Given the scope and scale of the project, it is therefore vital to set up a common, unique and spatio-semantically coherent urban model to be used as information hub for all applications being developed. This paper reports about the experiences done so far, it describes the test area and the available data sources, it shows and exemplifies the data integration issues, the strategies developed to solve them in order to obtain the integrated 3D city model. The first results as well as some comments about their quality and limitations are presented, together with the discussion regarding the next steps and some planned improvements.

  2. THREE DIMENSIONAL INTEGRATED CHARACTERIZATION AND ARCHIVING SYSTEM (3D-ICAS)

    SciTech Connect

    George Jarvis

    2001-06-18

    The overall objective of this project is to develop an integrated system that remotely characterizes, maps, and archives measurement data of hazardous decontamination and decommissioning (D&D) areas. The system will generate a detailed 3-dimensional topography of the area as well as real-time quantitative measurements of volatile organics and radionuclides. The system will analyze substrate materials consisting of concrete, asbestos, and transite. The system will permanently archive the data measurements for regulatory and data integrity documentation. Exposure limits, rest breaks, and donning and removal of protective garments generate waste in the form of contaminated protective garments and equipment. Survey times are increased and handling and transporting potentially hazardous materials incur additional costs. Off-site laboratory analysis is expensive and time-consuming, often necessitating delay of further activities until results are received. The Three Dimensional Integrated Characterization and Archiving System (3D-ICAS) has been developed to alleviate some of these problems. 3D-ICAS provides a flexible system for physical, chemical and nuclear measurements reduces costs and improves data quality. Operationally, 3D-ICAS performs real-time determinations of hazardous and toxic contamination. A prototype demonstration unit is available for use in early 2000. The tasks in this Phase included: (1) Mobility Platforms: Integrate hardware onto mobility platforms, upgrade surface sensors, develop unit operations and protocol. (2) System Developments: Evaluate metals detection capability using x-ray fluorescence technology. (3) IWOS Upgrades: Upgrade the IWOS software and hardware for compatibility with mobility platform. The system was modified, tested and debugged during 1999 and 2000. The 3D-ICAS was shipped on 11 May 2001 to FIU-HCET for demonstration and validation of the design modifications. These modifications included simplifying the design from a two

  3. Human Microtumors Generated in 3D: Novel Tools for Integrated In Situ Studies of Cancer Immunotherapies.

    PubMed

    Hambach, Lothar; Buser, Andreas; Vermeij, Marcel; Pouw, Nadine; van der Kwast, Theo; Goulmy, Els

    2016-01-01

    Cellular immunotherapy targeting human tumor antigens is a promising strategy to treat solid tumors. Yet clinical results of cellular immunotherapy are disappointing. Moreover, the currently available in vitro human tumor models are not designed to study the optimization of T-cell therapies of solid tumors. Here, we describe a novel assay for multiparametric in situ analysis of therapeutic effects on individual human three-dimensional (3D) tumors. In this assay, tumors of several millimeter diameter are generated from human cancer cell lines of different tumor entities in a collagen type I microenvironment. A newly developed approach for efficient morphological analysis reveals that these in vitro tumors resemble many characteristics of the corresponding clinical cancers such as histological features, immunohistochemical staining patterns, distinct tumor growth compartments and heterogeneous protein expression. To assess the response to therapy with tumor antigen specific T-cells, standardized protocols are described to determine T-cell infiltration and tumor destruction by monitoring soluble factors and tumor growth. Human tumors engineered in 3D collagen scaffolds are excellent in vitro surrogates for avascular tumor stages allowing integrated analyses of the antitumor efficacy of cancer specific immunotherapy in situ.

  4. Integrated Interventional Devices For Real Time 3D Ultrasound Imaging and Therapy

    NASA Astrophysics Data System (ADS)

    Smith, Stephen W.; Lee, Warren; Gentry, Kenneth L.; Pua, Eric C.; Light, Edward D.

    2006-05-01

    Two recent advances have expanded the potential of medical ultrasound: the introduction of real-time 3-D ultrasound imaging with catheter, transesophageal and laparoscopic probes and the development of interventional ultrasound therapeutic systems for focused ultrasound surgery, ablation and ultrasound enhanced drug delivery. This work describes devices combining both technologies. A series of transducer probes have been designed, fabricated and tested including: 1) a 12 French side scanning catheter incorporating a 64 element matrix array for imaging at 5MHz and a piston ablation transducer operating at 10 MHz. 2) a 14 Fr forward-scanning catheter integrating a 112 element 2-D array for imaging at 5 MHz encircled by an ablation annulus operating at 10 MHz. Finite element modeling was then used to simulate catheter annular and linear phased array transducers for ablation. 3) Linear phased array transducers were built to confirm the finite element analysis at 4 and 8 MHz including a mechanically focused 86 element 9 MHz array which transmits an ISPTA of 29.3 W/cm2 and creates a lesion in 2 minutes. 4) 2-D arrays of 504 channels operating at 5 MHz have been developed for transesophageal and laparascopic 3D imaging as well as therapeutic heating. All the devices image the heart anatomy including atria, valves, septa and en face views of the pulmonary veins.

  5. The cross-correlation between 3D cosmic shear and the integrated Sachs-Wolfe effect

    NASA Astrophysics Data System (ADS)

    Zieser, Britta; Merkel, Philipp M.

    2016-06-01

    We present the first calculation of the cross-correlation between 3D cosmic shear and the integrated Sachs-Wolfe (iSW) effect. Both signals are combined in a single formalism, which permits the computation of the full covariance matrix. In order to avoid the uncertainties presented by the non-linear evolution of the matter power spectrum and intrinsic alignments of galaxies, our analysis is restricted to large scales, i.e. multipoles below ℓ = 1000. We demonstrate in a Fisher analysis that this reduction compared to other studies of 3D weak lensing extending to smaller scales is compensated by the information that is gained if the additional iSW signal and in particular its cross-correlation with lensing data are considered. Given the observational standards of upcoming weak-lensing surveys like Euclid, marginal errors on cosmological parameters decrease by 10 per cent compared to a cosmic shear experiment if both types of information are combined without a cosmic wave background (CMB) prior. Once the constraining power of CMB data is added, the improvement becomes marginal.

  6. CATIA-V 3D Modeling for Design Integration of the Ignitor Machine Load Assembly^*

    NASA Astrophysics Data System (ADS)

    Bianchi, A.; Parodi, B.; Gardella, F.; Coppi, B.

    2007-11-01

    In the framework of the ANSALDO industrial contribution to the Ignitor engineering design, the detailed design of all components of the machine core (Load Assembly) has been completed. The machine Central Post, Central Solenoid, and Poloidal Field Coil systems, the Plasma Chamber and First Wall system, the surrounding mechanical structures, the Vacuum Cryostat and the polyethylene boron sheets attached to it for neutron shielding, have all been analyzed to confirm that they can withstand both normal and off-normal operating loads, as well as the Plasma Chamber and First Wall baking operations, with proper safety margins, for the maximum plasma parameters scenario at 13 T/11 MA, for the reduced scenarios at 9 T/7 MA (limiter) and at 9 T/6 MA (double nul). Both 3D and 2D drawings of each individual component have been produced using the Dassault Systems CATIA-V software. After they have been all integrated into a single 3D CATIA model of the Load Assembly, the electro-fluidic and fluidic lines which supply electrical currents and helium cooling gas to the coils have been added and mechanically incorporated with the components listed above. A global seismic analysis of the Load Assembly with SSE/OBE response spectra has also been performed to verify that it is able to withstand such external events. ^*Work supported in part by ENEA of italy and by the US D.O.E.

  7. Thin-dielectric-layer engineering for 3D nanostructure integration using an innovative planarization approach

    NASA Astrophysics Data System (ADS)

    Guerfi, Y.; Doucet, J. B.; Larrieu, G.

    2015-10-01

    Three-dimensional (3D) nanostructures are emerging as promising building blocks for a large spectrum of applications. One critical issue in integration regards mastering the thin, flat, and chemically stable insulating layer that must be implemented on the nanostructure network in order to build striking nano-architectures. In this letter, we report an innovative method for nanoscale planarization on 3D nanostructures by using hydrogen silesquioxane as a spin-on-glass (SOG) dielectric material. To decouple the thickness of the final layer from the height of the nanostructure, we propose to embed the nanowire network in the insulator layer by exploiting the planarizing properties of the SOG approach. To achieve the desired dielectric thickness, the structure is chemically etched back with a highly diluted solution to control the etch rate precisely. The roughness of the top surface was less than 2 nm. There were no surface defects and the planarity was excellent, even in the vicinity of the nanowires. This newly developed process was used to realize a multilevel stack architecture with sub-deca-nanometer-range layer thickness.

  8. Multiplexed polymer surface plasmon sensor with integrated optical coupler

    NASA Astrophysics Data System (ADS)

    Pyo, Hyeon-Bong; Park, Se Ho; Chung, Kwang Hyo; Choi, Chang Auck

    2005-11-01

    In this paper, we describe a novel multiplexed surface plasmon resonance (SPR) sensor which is made of cyclic olefin copolymers (COCs, TOPAS TM). This material has excellent chemical resistance, low water uptake (< 0.01%), and high refractive index (n He- Ne=1.53) suitable to use as an optical coupler (prism) as well as a sensor substrate. We fabricated a standard slide glass sized, prism integrated, and injection molded COC-SPR sensor which are being applied toward the multiplexed detection of DNA single nucleotide polymorphism (SNP). To evaluate the sensitivity of COC-SPR sensor, we first patterned MgF II on gold-coated COC-SPR sensor and observed the shift of minimum reflectivity (SPR dip) in pixel address. As incident light source we used an expanded, collimated, rectangular shaped He-Ne laser, with a diffuser for beam homogenization. With expanded laser beam we varied incident angle so that the angular shift is expressed as the darkest pixel shift on CCD. For optimized SPR characteristics and sensor configuration, analytical calculations (Fresnel equation) were performed, and the best SPR conditions were found to be d Au~48 nm at wavelength λ=633 nm with respected resonance angle at θ SPR =44.2° for COC-SPR sensor.

  9. 3Disease Browser: A Web server for integrating 3D genome and disease-associated chromosome rearrangement data

    PubMed Central

    Li, Ruifeng; Liu, Yifang; Li, Tingting; Li, Cheng

    2016-01-01

    Chromosomal rearrangement (CR) events have been implicated in many tumor and non-tumor human diseases. CR events lead to their associated diseases by disrupting gene and protein structures. Also, they can lead to diseases through changes in chromosomal 3D structure and gene expression. In this study, we search for CR-associated diseases potentially caused by chromosomal 3D structure alteration by integrating Hi-C and ChIP-seq data. Our algorithm rediscovers experimentally verified disease-associated CRs (polydactyly diseases) that alter gene expression by disrupting chromosome 3D structure. Interestingly, we find that intellectual disability may be a candidate disease caused by 3D chromosome structure alteration. We also develop a Web server (3Disease Browser, http://3dgb.cbi.pku.edu.cn/disease/) for integrating and visualizing disease-associated CR events and chromosomal 3D structure. PMID:27734896

  10. 3D integration of microcomponents in a single glass chip by femtosecond laser direct writing for biochemical analysis

    NASA Astrophysics Data System (ADS)

    Sugioka, Koji; Hanada, Yasutaka; Midorikawa, Katsumi

    2007-05-01

    3D integration of microcomponents in a single glass chip by femtosecond laser direct writing followed by post annealing and successive wet etching is described for application to biochemical analysis. Integration of microfluidics and microoptics realized some functional microdevices like a μ-fluidic dye laser and a biosensor. As one of practical applications, we demonstrate inspection of living microorganisms using the microchip with 3D microfluidic structures fabricated by the present technique.

  11. Real-time computer-generated integral imaging and 3D image calibration for augmented reality surgical navigation.

    PubMed

    Wang, Junchen; Suenaga, Hideyuki; Liao, Hongen; Hoshi, Kazuto; Yang, Liangjing; Kobayashi, Etsuko; Sakuma, Ichiro

    2015-03-01

    Autostereoscopic 3D image overlay for augmented reality (AR) based surgical navigation has been studied and reported many times. For the purpose of surgical overlay, the 3D image is expected to have the same geometric shape as the original organ, and can be transformed to a specified location for image overlay. However, how to generate a 3D image with high geometric fidelity and quantitative evaluation of 3D image's geometric accuracy have not been addressed. This paper proposes a graphics processing unit (GPU) based computer-generated integral imaging pipeline for real-time autostereoscopic 3D display, and an automatic closed-loop 3D image calibration paradigm for displaying undistorted 3D images. Based on the proposed methods, a novel AR device for 3D image surgical overlay is presented, which mainly consists of a 3D display, an AR window, a stereo camera for 3D measurement, and a workstation for information processing. The evaluation on the 3D image rendering performance with 2560×1600 elemental image resolution shows the rendering speeds of 50-60 frames per second (fps) for surface models, and 5-8 fps for large medical volumes. The evaluation of the undistorted 3D image after the calibration yields sub-millimeter geometric accuracy. A phantom experiment simulating oral and maxillofacial surgery was also performed to evaluate the proposed AR overlay device in terms of the image registration accuracy, 3D image overlay accuracy, and the visual effects of the overlay. The experimental results show satisfactory image registration and image overlay accuracy, and confirm the system usability.

  12. 3D Integration of MEMS and IC: Design, Technology and Simulations

    NASA Astrophysics Data System (ADS)

    Taklo, Maaike M. V.; Schjølberg-Henriksen, Kari; Lietaer, Nicolas; Prainsack, Josef; Elfving, Anders; Weber, Josef; Klein, Matthias; Schneider, Peter; Reitz, Sven

    A 3D integrated silicon stack consisting of two MEMS devices and two IC devices is presented. The MEMS devices are a pressure sensor and a bulk acoustic resonator (BAR). The stack was constructed for a tire pressure monitoring system (TPMS) which was one out of three demonstrators for an EU funded project called e-CUBES. Thermal simulations were performed to check the level of thermo-mechanical stresses induced on the pressure sensor membrane during extreme environmental conditions. Additional simulations were made to calculate the exact temperature on the BAR device during operation as this was important for the operational frequency. This paper presents and discusses the technology choices made for the stacking of the pressure sensor and the BAR. Results are given from simulations, initial short-loop experiments and for the final stacking.

  13. The RCSB protein data bank: integrative view of protein, gene and 3D structural information.

    PubMed

    Rose, Peter W; Prlić, Andreas; Altunkaya, Ali; Bi, Chunxiao; Bradley, Anthony R; Christie, Cole H; Costanzo, Luigi Di; Duarte, Jose M; Dutta, Shuchismita; Feng, Zukang; Green, Rachel Kramer; Goodsell, David S; Hudson, Brian; Kalro, Tara; Lowe, Robert; Peisach, Ezra; Randle, Christopher; Rose, Alexander S; Shao, Chenghua; Tao, Yi-Ping; Valasatava, Yana; Voigt, Maria; Westbrook, John D; Woo, Jesse; Yang, Huangwang; Young, Jasmine Y; Zardecki, Christine; Berman, Helen M; Burley, Stephen K

    2017-01-04

    The Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB PDB, http://rcsb.org), the US data center for the global PDB archive, makes PDB data freely available to all users, from structural biologists to computational biologists and beyond. New tools and resources have been added to the RCSB PDB web portal in support of a 'Structural View of Biology.' Recent developments have improved the User experience, including the high-speed NGL Viewer that provides 3D molecular visualization in any web browser, improved support for data file download and enhanced organization of website pages for query, reporting and individual structure exploration. Structure validation information is now visible for all archival entries. PDB data have been integrated with external biological resources, including chromosomal position within the human genome; protein modifications; and metabolic pathways. PDB-101 educational materials have been reorganized into a searchable website and expanded to include new features such as the Geis Digital Archive.

  14. 3D-NTT: a versatile integral field spectro-imager for the NTT

    NASA Astrophysics Data System (ADS)

    Marcelin, M.; Amram, P.; Balard, P.; Balkowski, C.; Boissin, O.; Boulesteix, J.; Carignan, C.; Daigle, O.; de Denus Baillargeon, M.-M.; Epinat, B.; Gach, J.-L.; Hernandez, O.; Rigaud, F.; Vallée, P.

    2008-07-01

    The 3D-NTT is a visible integral field spectro-imager offering two modes. A low resolution mode (R ~ 300 to 6 000) with a large field of view Tunable Filter (17'x17') and a high resolution mode (R ~ 10 000 to 40 000) with a scanning Fabry-Perot (7'x7'). It will be operated as a visitor instrument on the NTT from 2009. Two large programmes will be led: "Characterizing the interstellar medium of nearby galaxies with 2D maps of extinction and abundances" (PI M. Marcelin) and "Gas accretion and radiative feedback in the early universe" (PI J. Bland Hawthorn). Both will be mainly based on the Tunable Filter mode. This instrument is being built as a collaborative effort between LAM (Marseille), GEPI (Paris) and LAE (Montreal). The website adress of the instrument is : http://www.astro.umontreal.ca/3DNTT

  15. Tensile Properties and Failure Mechanism of 3D Woven Hollow Integrated Sandwich Composites

    NASA Astrophysics Data System (ADS)

    Liu, Chang; Cai, Deng'an; Zhou, Guangming; Lu, Fangzhou

    2017-01-01

    Tensile properties and failure mechanism of 3D woven hollow integrated sandwich composites are investigated experimentally, theoretically and numerically in this paper. Firstly, the tensile properties are obtained by quasi-static tensile tests on the specimens in two principal directions of the sandwich panels, called warp and weft. The experimental results shows that the tensile performances of the warp are better than that of the weft. By observing the broken specimens, it is found that the touch parts between yarns are the main failure regions under tension. Then, a theoretical method is developed to predict the tensile properties. By comparing with the experimental data, the accuracy of the theoretical method is verified. Simultaneously, a finite element model is established to predict the tensile behavior of the composites. The numerical results agree well with the experimental data. Moreover, the simulated progressive damages show that the contact regions in the warp and weft tension are both the initial failure areas.

  16. Anisotropic heat transport in integrable and chaotic 3-D magnetic fields

    SciTech Connect

    Del-Castillo-Negrete, Diego B; Blazevski, D.; Chacon, Luis

    2012-01-01

    A study of anisotropic heat transport in 3-D chaotic magnetic fields is presented. The approach is based on the recently proposed Lagrangian-Green s function (LG) method in Ref. [1] that allows an efficient and accurate integration of the parallel transport equation applicable to general magnetic fields with local or non-local parallel flux closures. We focus on reversed shear magnetic field configurations known to exhibit separatrix reconnection and shearless transport barriers. The role of reconnection and magnetic field line chaos on temperature transport is studied. Numerical results are presented on the anomalous relaxation of radial temperature gradients in the presence of shearless Cantori partial barri- ers. Also, numerical evidence of non-local effective radial temperature transport in chaotic fields is presented. Going beyond purely parallel transport, the LG method is generalized to include finite perpendicular diffusivity, and the problem of temperature flattening inside a magnetic island is studied.

  17. Integration of the virtual 3D model of a control system with the virtual controller

    NASA Astrophysics Data System (ADS)

    Herbuś, K.; Ociepka, P.

    2015-11-01

    Nowadays the design process includes simulation analysis of different components of a constructed object. It involves the need for integration of different virtual object to simulate the whole investigated technical system. The paper presents the issues related to the integration of a virtual 3D model of a chosen control system of with a virtual controller. The goal of integration is to verify the operation of an adopted object of in accordance with the established control program. The object of the simulation work is the drive system of a tunneling machine for trenchless work. In the first stage of work was created an interactive visualization of functioning of the 3D virtual model of a tunneling machine. For this purpose, the software of the VR (Virtual Reality) class was applied. In the elaborated interactive application were created adequate procedures allowing controlling the drive system of a translatory motion, a rotary motion and the drive system of a manipulator. Additionally was created the procedure of turning on and off the output crushing head, mounted on the last element of the manipulator. In the elaborated interactive application have been established procedures for receiving input data from external software, on the basis of the dynamic data exchange (DDE), which allow controlling actuators of particular control systems of the considered machine. In the next stage of work, the program on a virtual driver, in the ladder diagram (LD) language, was created. The control program was developed on the basis of the adopted work cycle of the tunneling machine. The element integrating the virtual model of the tunneling machine for trenchless work with the virtual controller is the application written in a high level language (Visual Basic). In the developed application was created procedures responsible for collecting data from the running, in a simulation mode, virtual controller and transferring them to the interactive application, in which is verified the

  18. Integrated gravity and gravity gradient 3D inversion using the non-linear conjugate gradient

    NASA Astrophysics Data System (ADS)

    Qin, Pengbo; Huang, Danian; Yuan, Yuan; Geng, Meixia; Liu, Jie

    2016-03-01

    Gravity data, which are critical in mineral, oil, and gas exploration, are obtained from the vertical component of the gravity field, while gravity gradient data are measured from changes in the gravity field in three directions. However, few studies have sought to improve exploration techniques by integrating gravity and gravity gradient data using inversion methods. In this study, we developed a new method to integrate gravity and gravity gradient data in a 3D density inversion using the non-linear conjugate gradient (NLCG) method and the minimum gradient support (MGS) functional to regularize the 3D inverse problem and to obtain a clear and accurate image of the anomalous body. The NLCG algorithm, which is suitable for solving large-scale nonlinear optimization problems and requires no memory storage, was compared to the Broyden-Fletcher-Goldfarb-Shanno (BFGS) quasi-Newton algorithm and the results indicated that the convergence rate of NLCG is slower, but that the storage requirement and computation time is lower. To counteract the decay in kernel function, we introduced a depth weighting function for anomalous bodies at the same depth, with information about anomalous body depth obtained from well log and seismic exploration data. For anomalous bodies at different depths, we introduced a spatial gradient weighting function to incorporate additional information obtained in the inversion. We concluded that the spatial gradient weighting function enhanced the spatial resolution of the recovered model. Furthermore, our results showed that including multiple components for inversion increased the resolution of the recovered model. We validated our model by applying our inversion method to survey data from Vinton salt dome, Louisiana, USA. The results showed good agreement with known geologic information; thus confirming the accuracy of this approach.

  19. Time And Temperature Dependent Micromechanical Properties Of Solder Joints For 3D-Package Integration

    NASA Astrophysics Data System (ADS)

    Roellig, Mike; Meier, Karsten; Metasch, Rene

    2010-11-01

    The recent development of 3D-integrated electronic packages is characterized by the need to increase the diversity of functions and to miniaturize. Currently many 3D-integration concepts are being developed and all of them demand new materials, new designs and new processing technologies. The combination of simulation and experimental investigation becomes increasingly accepted since simulations help to shorten the R&D cycle time and reduce costs. Numerical calculations like the Finite-Element-Method are strong tools to calculate stress conditions in electronic packages resulting from thermal strains due to the manufacturing process and environmental loads. It is essential for the application of numerical calculations that the material data is accurate and describes sufficiently the physical behaviour. The developed machine allows the measurement of time and temperature dependent micromechanical properties of solder joints. Solder joints, which are used to mechanically and electrically connect different packages, are physically measured as they leave the process. This allows accounting for process influences, which may change material properties. Additionally, joint sizes and metallurgical interactions between solder and under bump metallization can be respected by this particular measurement. The measurement allows the determination of material properties within a temperature range of 20° C-200° C. Further, the time dependent creep deformation can be measured within a strain-rate range of 10-31/s-10-81/s. Solder alloys based on Sn-Ag/Sn-Ag-Cu with additionally impurities and joint sizes down to O/ 200 μm were investigated. To finish the material characterization process the material model coefficient were extracted by FEM-Simulation to increase the accuracy of data.

  20. Integrating genomic information with protein sequence and 3D atomic level structure at the RCSB protein data bank.

    PubMed

    Prlić, Andreas; Kalro, Tara; Bhattacharya, Roshni; Christie, Cole; Burley, Stephen K; Rose, Peter W

    2016-12-15

    The Protein Data Bank (PDB) now contains more than 120,000 three-dimensional (3D) structures of biological macromolecules. To allow an interpretation of how PDB data relates to other publicly available annotations, we developed a novel data integration platform that maps 3D structural information across various datasets. This integration bridges from the human genome across protein sequence to 3D structure space. We developed novel software solutions for data management and visualization, while incorporating new libraries for web-based visualization using SVG graphics.

  1. A 3D bioprinting system to produce human-scale tissue constructs with structural integrity.

    PubMed

    Kang, Hyun-Wook; Lee, Sang Jin; Ko, In Kap; Kengla, Carlos; Yoo, James J; Atala, Anthony

    2016-03-01

    A challenge for tissue engineering is producing three-dimensional (3D), vascularized cellular constructs of clinically relevant size, shape and structural integrity. We present an integrated tissue-organ printer (ITOP) that can fabricate stable, human-scale tissue constructs of any shape. Mechanical stability is achieved by printing cell-laden hydrogels together with biodegradable polymers in integrated patterns and anchored on sacrificial hydrogels. The correct shape of the tissue construct is achieved by representing clinical imaging data as a computer model of the anatomical defect and translating the model into a program that controls the motions of the printer nozzles, which dispense cells to discrete locations. The incorporation of microchannels into the tissue constructs facilitates diffusion of nutrients to printed cells, thereby overcoming the diffusion limit of 100-200 μm for cell survival in engineered tissues. We demonstrate capabilities of the ITOP by fabricating mandible and calvarial bone, cartilage and skeletal muscle. Future development of the ITOP is being directed to the production of tissues for human applications and to the building of more complex tissues and solid organs.

  2. Fully 3D-Integrated Pixel Detectors for X-Rays

    SciTech Connect

    Deptuch, Grzegorz W.; Gabriella, Carini; Enquist, Paul; Grybos, Pawel; Holm, Scott; Lipton, Ronald; Maj, Piotr; Patti, Robert; Siddons, David Peter; Szczygiel, Robert; Yarema, Raymond

    2016-01-01

    The vertically integrated photon imaging chip (VIPIC1) pixel detector is a stack consisting of a 500-μm-thick silicon sensor, a two-tier 34-μm-thick integrated circuit, and a host printed circuit board (PCB). The integrated circuit tiers were bonded using the direct bonding technology with copper, and each tier features 1-μm-diameter through-silicon vias that were used for connections to the sensor on one side, and to the host PCB on the other side. The 80-μm-pixel-pitch sensor was the direct bonding technology with nickel bonded to the integrated circuit. The stack was mounted on the board using Sn–Pb balls placed on a 320-μm pitch, yielding an entirely wire-bond-less structure. The analog front-end features a pulse response peaking at below 250 ns, and the power consumption per pixel is 25 μW. We successful completed the 3-D integration and have reported here. Additionally, all pixels in the matrix of 64 × 64 pixels were responding on well-bonded devices. Correct operation of the sparsified readout, allowing a single 153-ns bunch timing resolution, was confirmed in the tests on a synchrotron beam of 10-keV X-rays. An equivalent noise charge of 36.2 e- rms and a conversion gain of 69.5 μV/e- with 2.6 e- rms and 2.7 μV/e- rms pixel-to-pixel variations, respectively, were measured.

  3. Fully 3D-Integrated Pixel Detectors for X-Rays

    DOE PAGES

    Deptuch, Grzegorz W.; Gabriella, Carini; Enquist, Paul; ...

    2016-01-01

    The vertically integrated photon imaging chip (VIPIC1) pixel detector is a stack consisting of a 500-μm-thick silicon sensor, a two-tier 34-μm-thick integrated circuit, and a host printed circuit board (PCB). The integrated circuit tiers were bonded using the direct bonding technology with copper, and each tier features 1-μm-diameter through-silicon vias that were used for connections to the sensor on one side, and to the host PCB on the other side. The 80-μm-pixel-pitch sensor was the direct bonding technology with nickel bonded to the integrated circuit. The stack was mounted on the board using Sn–Pb balls placed on a 320-μm pitch,more » yielding an entirely wire-bond-less structure. The analog front-end features a pulse response peaking at below 250 ns, and the power consumption per pixel is 25 μW. We successful completed the 3-D integration and have reported here. Additionally, all pixels in the matrix of 64 × 64 pixels were responding on well-bonded devices. Correct operation of the sparsified readout, allowing a single 153-ns bunch timing resolution, was confirmed in the tests on a synchrotron beam of 10-keV X-rays. An equivalent noise charge of 36.2 e- rms and a conversion gain of 69.5 μV/e- with 2.6 e- rms and 2.7 μV/e- rms pixel-to-pixel variations, respectively, were measured.« less

  4. Integrative multicellular biological modeling: a case study of 3D epidermal development using GPU algorithms

    PubMed Central

    2010-01-01

    Background Simulation of sophisticated biological models requires considerable computational power. These models typically integrate together numerous biological phenomena such as spatially-explicit heterogeneous cells, cell-cell interactions, cell-environment interactions and intracellular gene networks. The recent advent of programming for graphical processing units (GPU) opens up the possibility of developing more integrative, detailed and predictive biological models while at the same time decreasing the computational cost to simulate those models. Results We construct a 3D model of epidermal development and provide a set of GPU algorithms that executes significantly faster than sequential central processing unit (CPU) code. We provide a parallel implementation of the subcellular element method for individual cells residing in a lattice-free spatial environment. Each cell in our epidermal model includes an internal gene network, which integrates cellular interaction of Notch signaling together with environmental interaction of basement membrane adhesion, to specify cellular state and behaviors such as growth and division. We take a pedagogical approach to describing how modeling methods are efficiently implemented on the GPU including memory layout of data structures and functional decomposition. We discuss various programmatic issues and provide a set of design guidelines for GPU programming that are instructive to avoid common pitfalls as well as to extract performance from the GPU architecture. Conclusions We demonstrate that GPU algorithms represent a significant technological advance for the simulation of complex biological models. We further demonstrate with our epidermal model that the integration of multiple complex modeling methods for heterogeneous multicellular biological processes is both feasible and computationally tractable using this new technology. We hope that the provided algorithms and source code will be a starting point for modelers to

  5. Surgical Navigation Technology Based on Augmented Reality and Integrated 3D Intraoperative Imaging

    PubMed Central

    Elmi-Terander, Adrian; Skulason, Halldor; Söderman, Michael; Racadio, John; Homan, Robert; Babic, Drazenko; van der Vaart, Nijs; Nachabe, Rami

    2016-01-01

    Study Design. A cadaveric laboratory study. Objective. The aim of this study was to assess the feasibility and accuracy of thoracic pedicle screw placement using augmented reality surgical navigation (ARSN). Summary of Background Data. Recent advances in spinal navigation have shown improved accuracy in lumbosacral pedicle screw placement but limited benefits in the thoracic spine. 3D intraoperative imaging and instrument navigation may allow improved accuracy in pedicle screw placement, without the use of x-ray fluoroscopy, and thus opens the route to image-guided minimally invasive therapy in the thoracic spine. Methods. ARSN encompasses a surgical table, a motorized flat detector C-arm with intraoperative 2D/3D capabilities, integrated optical cameras for augmented reality navigation, and noninvasive patient motion tracking. Two neurosurgeons placed 94 pedicle screws in the thoracic spine of four cadavers using ARSN on one side of the spine (47 screws) and free-hand technique on the contralateral side. X-ray fluoroscopy was not used for either technique. Four independent reviewers assessed the postoperative scans, using the Gertzbein grading. Morphometric measurements of the pedicles axial and sagittal widths and angles, as well as the vertebrae axial and sagittal rotations were performed to identify risk factors for breaches. Results. ARSN was feasible and superior to free-hand technique with respect to overall accuracy (85% vs. 64%, P < 0.05), specifically significant increases of perfectly placed screws (51% vs. 30%, P < 0.05) and reductions in breaches beyond 4 mm (2% vs. 25%, P < 0.05). All morphometric dimensions, except for vertebral body axial rotation, were risk factors for larger breaches when performed with the free-hand method. Conclusion. ARSN without fluoroscopy was feasible and demonstrated higher accuracy than free-hand technique for thoracic pedicle screw placement. Level of Evidence: N/A PMID:27513166

  6. Superresolution of 3-D computational integral imaging based on moving least square method.

    PubMed

    Kim, Hyein; Lee, Sukho; Ryu, Taekyung; Yoon, Jungho

    2014-11-17

    In this paper, we propose an edge directive moving least square (ED-MLS) based superresolution method for computational integral imaging reconstruction(CIIR). Due to the low resolution of the elemental images and the alignment error of the microlenses, it is not easy to obtain an accurate registration result in integral imaging, which makes it difficult to apply superresolution to the CIIR application. To overcome this problem, we propose the edge directive moving least square (ED-MLS) based superresolution method which utilizes the properties of the moving least square. The proposed ED-MLS based superresolution takes the direction of the edge into account in the moving least square reconstruction to deal with the abrupt brightness changes in the edge regions, and is less sensitive to the registration error. Furthermore, we propose a framework which shows how the data have to be collected for the superresolution problem in the CIIR application. Experimental results verify that the resolution of the elemental images is enhanced, and that a high resolution reconstructed 3-D image can be obtained with the proposed method.

  7. NFkB disrupts tissue polarity in 3D by preventing integration of microenvironmental signals

    PubMed Central

    Xiong, Gaofeng; Furuta, Saori; Han, Ju; Kuhn, Irene; Akavia, Uri-David; Pe'er, Dana; Bissell, Mina J

    2013-01-01

    The microenvironment of cells controls their phenotype, and thereby the architecture of the emerging multicellular structure or tissue. We have reported more than a dozen microenvironmental factors whose signaling must be integrated in order to effect an organized, functional tissue morphology. However, the factors that prevent integration of signaling pathways that merge form and function are still largely unknown. We have identified nuclear factor kappa B (NFkB) as a transcriptional regulator that disrupts important microenvironmental cues necessary for tissue organization. We compared the gene expression of organized and disorganized epithelial cells of the HMT-3522 breast cancer progression series: the non-malignant S1 cells that form polarized spheres (‘acini’), the malignant T4-2 cells that form large tumor-like clusters, and the ‘phenotypically reverted’ T4-2 cells that polarize as a result of correction of the microenvironmental signaling. We identified 180 genes that display an increased expression in disorganized compared to polarized structures. Network, GSEA and transcription factor binding site analyses suggested that NFkB is a common activator for the 180 genes. NFkB was found to be activated in disorganized breast cancer cells, and inhibition of microenvironmental signaling via EGFR, beta1 integrin, MMPs, or their downstream signals suppressed its activation. The postulated role of NFkB was experimentally verified: Blocking the NFkB pathway with a specific chemical inhibitor or shRNA induced polarization and inhibited invasion of breast cancer cells in 3D cultures. These results may explain why NFkB holds promise as a target for therapeutic intervention: Its inhibition can reverse the oncogenic signaling involved in breast cancer progression and integrate the essential microenvironmental control of tissue architecture. PMID:24243820

  8. Integrating 3D geological information with a national physically-based hydrological modelling system

    NASA Astrophysics Data System (ADS)

    Lewis, Elizabeth; Parkin, Geoff; Kessler, Holger; Whiteman, Mark

    2016-04-01

    Robust numerical models are an essential tool for informing flood and water management and policy around the world. Physically-based hydrological models have traditionally not been used for such applications due to prohibitively large data, time and computational resource requirements. Given recent advances in computing power and data availability, a robust, physically-based hydrological modelling system for Great Britain using the SHETRAN model and national datasets has been created. Such a model has several advantages over less complex systems. Firstly, compared with conceptual models, a national physically-based model is more readily applicable to ungauged catchments, in which hydrological predictions are also required. Secondly, the results of a physically-based system may be more robust under changing conditions such as climate and land cover, as physical processes and relationships are explicitly accounted for. Finally, a fully integrated surface and subsurface model such as SHETRAN offers a wider range of applications compared with simpler schemes, such as assessments of groundwater resources, sediment and nutrient transport and flooding from multiple sources. As such, SHETRAN provides a robust means of simulating numerous terrestrial system processes which will add physical realism when coupled to the JULES land surface model. 306 catchments spanning Great Britain have been modelled using this system. The standard configuration of this system performs satisfactorily (NSE > 0.5) for 72% of catchments and well (NSE > 0.7) for 48%. Many of the remaining 28% of catchments that performed relatively poorly (NSE < 0.5) are located in the chalk in the south east of England. As such, the British Geological Survey 3D geology model for Great Britain (GB3D) has been incorporated, for the first time in any hydrological model, to pave the way for improvements to be made to simulations of catchments with important groundwater regimes. This coupling has involved

  9. The integration of 3-D cell printing and mesoscopic fluorescence molecular tomography of vascular constructs within thick hydrogel scaffolds.

    PubMed

    Zhao, Lingling; Lee, Vivian K; Yoo, Seung-Schik; Dai, Guohao; Intes, Xavier

    2012-07-01

    Developing methods that provide adequate vascular perfusion is an important step toward engineering large functional tissues. Meanwhile, an imaging modality to assess the three-dimensional (3-D) structures and functions of the vascular channels is lacking for thick matrices (>2 ≈ 3 mm). Herein, we report on an original approach to construct and image 3-D dynamically perfused vascular structures in thick hydrogel scaffolds. In this work, we integrated a robotic 3-D cell printing technology with a mesoscopic fluorescence molecular tomography imaging system, and demonstrated the capability of the platform to construct perfused collagen scaffolds with endothelial lining and to image both the fluid flow and fluorescent-labeled living endothelial cells at high-frame rates, with high sensitivity and accuracy. These results establish the potential of integrating both 3-D cell printing and fluorescence mesoscopic imaging for functional and molecular studies in complex tissue-engineered tissues.

  10. Laser Scanning Holographic Lithography for Flexible 3D Fabrication of Multi-Scale Integrated Nano-structures and Optical Biosensors.

    PubMed

    Yuan, Liang Leon; Herman, Peter R

    2016-02-29

    Three-dimensional (3D) periodic nanostructures underpin a promising research direction on the frontiers of nanoscience and technology to generate advanced materials for exploiting novel photonic crystal (PC) and nanofluidic functionalities. However, formation of uniform and defect-free 3D periodic structures over large areas that can further integrate into multifunctional devices has remained a major challenge. Here, we introduce a laser scanning holographic method for 3D exposure in thick photoresist that combines the unique advantages of large area 3D holographic interference lithography (HIL) with the flexible patterning of laser direct writing to form both micro- and nano-structures in a single exposure step. Phase mask interference patterns accumulated over multiple overlapping scans are shown to stitch seamlessly and form uniform 3D nanostructure with beam size scaled to small 200 μm diameter. In this way, laser scanning is presented as a facile means to embed 3D PC structure within microfluidic channels for integration into an optofluidic lab-on-chip, demonstrating a new laser HIL writing approach for creating multi-scale integrated microsystems.

  11. Laser Scanning Holographic Lithography for Flexible 3D Fabrication of Multi-Scale Integrated Nano-structures and Optical Biosensors

    NASA Astrophysics Data System (ADS)

    Yuan, Liang (Leon); Herman, Peter R.

    2016-02-01

    Three-dimensional (3D) periodic nanostructures underpin a promising research direction on the frontiers of nanoscience and technology to generate advanced materials for exploiting novel photonic crystal (PC) and nanofluidic functionalities. However, formation of uniform and defect-free 3D periodic structures over large areas that can further integrate into multifunctional devices has remained a major challenge. Here, we introduce a laser scanning holographic method for 3D exposure in thick photoresist that combines the unique advantages of large area 3D holographic interference lithography (HIL) with the flexible patterning of laser direct writing to form both micro- and nano-structures in a single exposure step. Phase mask interference patterns accumulated over multiple overlapping scans are shown to stitch seamlessly and form uniform 3D nanostructure with beam size scaled to small 200 μm diameter. In this way, laser scanning is presented as a facile means to embed 3D PC structure within microfluidic channels for integration into an optofluidic lab-on-chip, demonstrating a new laser HIL writing approach for creating multi-scale integrated microsystems.

  12. Laser Scanning Holographic Lithography for Flexible 3D Fabrication of Multi-Scale Integrated Nano-structures and Optical Biosensors

    PubMed Central

    Yuan, Liang (Leon); Herman, Peter R.

    2016-01-01

    Three-dimensional (3D) periodic nanostructures underpin a promising research direction on the frontiers of nanoscience and technology to generate advanced materials for exploiting novel photonic crystal (PC) and nanofluidic functionalities. However, formation of uniform and defect-free 3D periodic structures over large areas that can further integrate into multifunctional devices has remained a major challenge. Here, we introduce a laser scanning holographic method for 3D exposure in thick photoresist that combines the unique advantages of large area 3D holographic interference lithography (HIL) with the flexible patterning of laser direct writing to form both micro- and nano-structures in a single exposure step. Phase mask interference patterns accumulated over multiple overlapping scans are shown to stitch seamlessly and form uniform 3D nanostructure with beam size scaled to small 200 μm diameter. In this way, laser scanning is presented as a facile means to embed 3D PC structure within microfluidic channels for integration into an optofluidic lab-on-chip, demonstrating a new laser HIL writing approach for creating multi-scale integrated microsystems. PMID:26922872

  13. Swarm Intelligence Integrated Graph-Cut for Liver Segmentation from 3D-CT Volumes

    PubMed Central

    Eapen, Maya; Korah, Reeba; Geetha, G.

    2015-01-01

    The segmentation of organs in CT volumes is a prerequisite for diagnosis and treatment planning. In this paper, we focus on liver segmentation from contrast-enhanced abdominal CT volumes, a challenging task due to intensity overlapping, blurred edges, large variability in liver shape, and complex background with cluttered features. The algorithm integrates multidiscriminative cues (i.e., prior domain information, intensity model, and regional characteristics of liver in a graph-cut image segmentation framework). The paper proposes a swarm intelligence inspired edge-adaptive weight function for regulating the energy minimization of the traditional graph-cut model. The model is validated both qualitatively (by clinicians and radiologists) and quantitatively on publically available computed tomography (CT) datasets (MICCAI 2007 liver segmentation challenge, 3D-IRCAD). Quantitative evaluation of segmentation results is performed using liver volume calculations and a mean score of 80.8% and 82.5% on MICCAI and IRCAD dataset, respectively, is obtained. The experimental result illustrates the efficiency and effectiveness of the proposed method. PMID:26689833

  14. Mercury Lightcraft Project Update: 3-D Modeling, Systems Analysis and Integration

    NASA Astrophysics Data System (ADS)

    Buckton, Thomas W.; Myrabo, Leik N.

    2005-04-01

    This paper is a progress report on the laser-propelled Mercury Lightcraft Project at Rensselaer Polytechnic Institute. The laser-propelled, 1-person craft has a diameter of 252-cm, height of 217-cm, internal volume of 3 m3, `dry' mass of 700 kg, and gross liftoff mass of 1 metric ton. Expendable liquids including 70 kg of liquid hydrogen, and an equivalent mass (at least) of de-ionized water serves as open-cycle coolants for the 520 MWe laser/electric power conversion system. Its hyper-energetic airbreathing engine can easily accelerate the vehicle at 10 Gs or more. The tractor-beam lightcraft is intended as a prototype for use in a future global aerospace transportation system based on a constellation of satellite solar power stations in geostationary orbit, with laser relay stations in low Earth orbit. Using SolidWorks® 3-D modeling software, several important features were successfully integrated into the Mercury lightcraft model - principally: a rotating shroud (for spin stabilization) simple actuation system for a new variable-geometry air inlet; refined optical train for the laser-heated H2 plasma generators; pneumatically deployed, robotic quadra-pod landing gear; ejection seat/pod/hatch system; and a more detailed airframe structural concept. The CAD effort has brought the Mercury Lightcraft concept one significant step closer to reality.

  15. The RCSB protein data bank: integrative view of protein, gene and 3D structural information

    PubMed Central

    Rose, Peter W.; Prlić, Andreas; Altunkaya, Ali; Bi, Chunxiao; Bradley, Anthony R.; Christie, Cole H.; Costanzo, Luigi Di; Duarte, Jose M.; Dutta, Shuchismita; Feng, Zukang; Green, Rachel Kramer; Goodsell, David S.; Hudson, Brian; Kalro, Tara; Lowe, Robert; Peisach, Ezra; Randle, Christopher; Rose, Alexander S.; Shao, Chenghua; Tao, Yi-Ping; Valasatava, Yana; Voigt, Maria; Westbrook, John D.; Woo, Jesse; Yang, Huangwang; Young, Jasmine Y.; Zardecki, Christine; Berman, Helen M.; Burley, Stephen K.

    2017-01-01

    The Research Collaboratory for Structural Bioinformatics Protein Data Bank (RCSB PDB, http://rcsb.org), the US data center for the global PDB archive, makes PDB data freely available to all users, from structural biologists to computational biologists and beyond. New tools and resources have been added to the RCSB PDB web portal in support of a ‘Structural View of Biology.’ Recent developments have improved the User experience, including the high-speed NGL Viewer that provides 3D molecular visualization in any web browser, improved support for data file download and enhanced organization of website pages for query, reporting and individual structure exploration. Structure validation information is now visible for all archival entries. PDB data have been integrated with external biological resources, including chromosomal position within the human genome; protein modifications; and metabolic pathways. PDB-101 educational materials have been reorganized into a searchable website and expanded to include new features such as the Geis Digital Archive. PMID:27794042

  16. Liquid immersion thermal crosslinking of 3D polymer nanopatterns for direct carbonisation with high structural integrity

    PubMed Central

    Kang, Da-Young; Kim, Cheolho; Park, Gyurim; Moon, Jun Hyuk

    2015-01-01

    The direct pyrolytic carbonisation of polymer patterns has attracted interest for its use in obtaining carbon materials. In the case of carbonisation of nanopatterned polymers, the polymer flow and subsequent pattern change may occur in order to relieve their high surface energies. Here, we demonstrated that liquid immersion thermal crosslinking of polymer nanopatterns effectively enhanced the thermal resistance and maintained the structure integrity during the heat treatment. We employed the liquid immersion thermal crosslinking for 3D porous SU8 photoresist nanopatterns and successfully converted them to carbon nanopatterns while maintaining their porous features. The thermal crosslinking reaction and carbonisation of SU8 nanopatterns were characterised. The micro-crystallinity of the SU8-derived carbon nanopatterns was also characterised. The liquid immersion heat treatment can be extended to the carbonisation of various polymer or photoresist nanopatterns and also provide a facile way to control the surface energy of polymer nanopatterns for various purposes, for example, to block copolymer or surfactant self-assemblies. PMID:26677949

  17. Acoustic backing in 3-D integration of CMUT with front-end electronics.

    PubMed

    Berg, Sigrid; Rønnekleiv, Arne

    2012-07-01

    Capacitive micromachined ultrasonic transducers (CMUTs) have shown promising qualities for medical imaging. However, there are still some problems to be investigated, and some challenges to overcome. Acoustic backing is necessary to prevent SAWs excited in the surface of the silicon substrate from affecting the transmit pattern from the array. In addition, echoes resulting from bulk waves in the substrate must be removed. There is growing interest in integrating electronic circuits to do some of the beamforming directly below the transducer array. This may be easier to achieve for CMUTs than for traditional piezoelectric transducers. We will present simulations showing that the thickness of the silicon substrate and thicknesses and acoustic properties of the bonding material must be considered, especially when designing highfrequency transducers. Through simulations, we compare the acoustic properties of 3-D stacks bonded with three different bonding techniques; solid-liquid interdiffusion (SLID) bonding, direct fusion bonding, and anisotropic conductive adhesives (ACA). We look at a CMUT array with a center frequency of 30 MHz and three silicon wafers underneath, having a total silicon thickness of 100 μm. We find that fusion bonding is most beneficial if we want to prevent surface waves from damaging the array response, but SLID and ACA are also promising if bonding layer thicknesses can be reduced.

  18. From 3D Bioprinters to a fully integrated Organ Biofabrication Line

    NASA Astrophysics Data System (ADS)

    Passamai, V. E.; Dernowsek, J. A.; Nogueira, J.; Lara, V.; Vilalba, F.; Mironov, V. A.; Rezende, R. A.; da Silva, J. V.

    2016-04-01

    About 30 years ago, the 3D printing technique appeared. From that time on, engineers in medical science field started to look at 3D printing as a partner. Firstly, biocompatible and biodegradable 3D structures for cell seeding called “scaffolds” were fabricated for in vitro and in vivo animal trials. The advances proved to be of great importance, but, the use of scaffolds faces some limitations, such as low homogeneity and low density of cell aggregates. In the last decade, 3D bioprinting technology emerged as a promising approach to overcome these limitations and as one potential solution to the challenge of organ fabrication, to obtain very similar 3D human tissues, not only for transplantation, but also for drug discovery, disease research and to decrease the usage of animals in laboratory experimentation. 3D bioprinting allowed the fabrication of 3D alive structures with higher and controllable cell density and homogeneity. Other advantage of biofabrication is that the tissue constructs are solid scaffold-free. This paper presents the 3D bioprinting technology; equipment development, stages and components of a complex Organ Bioprinting Line (OBL) and the importance of developing a Virtual OBL.

  19. A polymer-based Fabry-Perot filter integrated with 3-D MEMS structures

    NASA Astrophysics Data System (ADS)

    Zhang, Ping (Cerina); Le, Kevin; Malalur-Nagaraja-Rao, Smitha; Hsu, Lun-Chen; Chiao, J.-C.

    2006-01-01

    Polymers have been considered as one of the most versatile materials in making optical devices for communication and sensor applications. They provide good optical transparency to form filters, lenses and many optical components with ease of fabrication. They are scalable and compatible in dimensions with requirements in optics and can be fabricated on inorganic substrates, such as silicon and quartz. Recent polymer synthesis also made great progresses on conductive and nonlinear polymers, opening opportunities for new applications. In this paper, we discussed hybrid-material integration of polymers on silicon-based microelectromechanical system (MEMS) devices. The motivation is to combine the advantages of demonstrated silicon-based MEMS actuators and excellent optical performance of polymers. We demonstrated the idea with a polymer-based out-of-plane Fabry-Perot filter that can be self-assembled by scratch drive actuators. We utilized a fabrication foundry service, MUMPS (Multi-User MEMS Process), to demonstrate the feasibility and flexibility of integration. The polysilicon, used as the structural material for construction of 3-D framework and actuators, has high absorption in the visible and near infrared ranges. Therefore, previous efforts using a polysilicon layer as optical interfaces suffer from high losses. We applied the organic compound materials on the silicon-based framework within the optical signal propagation path to form the optical interfaces. In this paper, we have shown low losses in the optical signal processing and feasibility of building a thin-film Fabry-Perot filter. We discussed the optical filter designs, mechanical design, actuation mechanism, fabrication issues, optical measurements, and results.

  20. Integrated Idl Tool For 3d Modeling And Imaging Data Analysis

    NASA Astrophysics Data System (ADS)

    Nita, Gelu M.; Fleishman, G. D.; Gary, D. E.; Kuznetsov, A. A.; Kontar, E. P.

    2012-05-01

    Addressing many key problems in solar physics requires detailed analysis of non-simultaneous imaging data obtained in various wavelength domains with different spatial resolution and their comparison with each other supplied by advanced 3D physical models. To facilitate achieving this goal, we have undertaken a major enhancement and improvements of IDL-based simulation tools developed earlier for modeling microwave and X-ray emission. The greatly enhanced object-based architecture provides interactive graphic user interface that allows the user i) to import photospheric magnetic field maps and perform magnetic field extrapolations to almost instantly generate 3D magnetic field models, ii) to investigate the magnetic topology of these models by interactively creating magnetic field lines and associated magnetic field tubes, iii) to populate them with user-defined nonuniform thermal plasma and anisotropic nonuniform nonthermal electron distributions; and iv) to calculate the spatial and spectral properties of radio and X-ray emission. The application integrates DLL and Shared Libraries containing fast gyrosynchrotron emission codes developed in FORTRAN and C++, soft and hard X-ray codes developed in IDL, and a potential field extrapolation DLL produced based on original FORTRAN code developed by V. Abramenko and V. Yurchishin. The interactive interface allows users to add any user-defined IDL or external callable radiation code, as well as user-defined magnetic field extrapolation routines. To illustrate the tool capabilities, we present a step-by-step live computation of microwave and X-ray images from realistic magnetic structures obtained from a magnetic field extrapolation preceding a real event, and compare them with the actual imaging data produced by NORH and RHESSI instruments. This work was supported in part by NSF grants AGS-0961867, AST-0908344, AGS-0969761, and NASA grants NNX10AF27G and NNX11AB49G to New Jersey Institute of Technology, by a UK STFC

  1. Interferometry based multispectral photon-limited 2D and 3D integral image encryption employing the Hartley transform.

    PubMed

    Muniraj, Inbarasan; Guo, Changliang; Lee, Byung-Geun; Sheridan, John T

    2015-06-15

    We present a method of securing multispectral 3D photon-counted integral imaging (PCII) using classical Hartley Transform (HT) based encryption by employing optical interferometry. This method has the simultaneous advantages of minimizing complexity by eliminating the need for holography recording and addresses the phase sensitivity problem encountered when using digital cameras. These together with single-channel multispectral 3D data compactness, the inherent properties of the classical photon counting detection model, i.e. sparse sensing and the capability for nonlinear transformation, permits better authentication of the retrieved 3D scene at various depth cues. Furthermore, the proposed technique works for both spatially and temporally incoherent illumination. To validate the proposed technique simulations were carried out for both the 2D and 3D cases. Experimental data is processed and the results support the feasibility of the encryption method.

  2. Full parallax viewing-angle enhanced computer-generated holographic 3D display system using integral lens array.

    PubMed

    Choi, Kyongsik; Kim, Joohwan; Lim, Yongjun; Lee, Byoungho

    2005-12-26

    A novel full parallax and viewing-angle enhanced computer-generated holographic (CGH) three-dimensional (3D) display system is proposed and implemented by combining an integral lens array and colorized synthetic phase holograms displayed on a phase-type spatial light modulator. For analyzing the viewing-angle limitations of our CGH 3D display system, we provide some theoretical background and introduce a simple ray-tracing method for 3D image reconstruction. From our method we can get continuously varying full parallax 3D images with the viewing angle about +/-6 degrees . To design the colorized phase holograms, we used a modified iterative Fourier transform algorithm and we could obtain a high diffraction efficiency (~92.5%) and a large signal-to-noise ratio (~11dB) from our simulation results. Finally we show some experimental results that verify our concept and demonstrate the full parallax viewing-angle enhanced color CGH display system.

  3. A Flat Interface Nerve Electrode With Integrated Multiplexer

    PubMed Central

    Lertmanorat, Zeng; Montague, F. W; Durand, Dominique M.

    2009-01-01

    One of the goals of peripheral nerve cuff electrode development is the design of an electrode capable of selectively activating a specific population of axons in a common nerve trunk. Several designs such as the round spiral electrode or the flat interface nerve electrode (FINE) have shown such ability. However, multiple contact electrodes require many leads, making the implantation difficult and potentially damaging to the nerve. Taking advantage of the flat geometry of the FINE, multiplexers were embedded within the cuff electrode to reduce the number of leads needed to control thirty two channels. The circuit was implemented on a polyimide film using off-the-shelf electronic components. The electronic module was surface-mounted directly onto the electrode’s flat substrate. Two circuit designs were designed, built and tested; 1) a single supply design with only two wires but limited to cathodic-first pulse and 2) a dual-supply design requiring three lead wires but an arbitrary stimulation waveform. The electrode design includes thirty-two contacts in a 1mm×8mm opening. The contact size is 300μm ×400μm with access resistance less than 1kΩ. This electrode is not intended for long-term use, but developed as a feasibility study for future development using low-water-absorption materials such as liquid crystal polymer and an application specific integrated circuit. PMID:19362897

  4. Developement of 3D Vertically Integrated Pattern Recognition Associative Memory (VIPRAM)

    SciTech Connect

    Deputch, G.; Hoff, J.; Lipton, R.; Liu, T.; Olsen, J.; Ramberg, E.; Wu, Jin-Yuan; Yarema, R.; Shochet, M.; Tang, F.; Demarteau, M.; /Argonne /INFN, Padova

    2011-04-13

    Many next-generation physics experiments will be characterized by the collection of large quantities of data, taken in rapid succession, from which scientists will have to unravel the underlying physical processes. In most cases, large backgrounds will overwhelm the physics signal. Since the quantity of data that can be stored for later analysis is limited, real-time event selection is imperative to retain the interesting events while rejecting the background. Scaling of current technologies is unlikely to satisfy the scientific needs of future projects, so investments in transformational new technologies need to be made. For example, future particle physics experiments looking for rare processes will have to address the demanding challenges of fast pattern recognition in triggering as detector hit density becomes significantly higher due to the high luminosity required to produce the rare processes. In this proposal, we intend to develop hardware-based technology that significantly advances the state-of-the-art for fast pattern recognition within and outside HEP using the 3D vertical integration technology that has emerged recently in industry. The ultimate physics reach of the LHC experiments will crucially depend on the tracking trigger's ability to help discriminate between interesting rare events and the background. Hardware-based pattern recognition for fast triggering on particle tracks has been successfully used in high-energy physics experiments for some time. The CDF Silicon Vertex Trigger (SVT) at the Fermilab Tevatron is an excellent example. The method used there, developed in the 1990's, is based on algorithms that use a massively parallel associative memory architecture to identify patterns efficiently at high speed. However, due to much higher occupancy and event rates at the LHC, and the fact that the LHC detectors have a much larger number of channels in their tracking detectors, there is an enormous challenge in implementing pattern recognition

  5. Parallel robot for micro assembly with integrated innovative optical 3D-sensor

    NASA Astrophysics Data System (ADS)

    Hesselbach, Juergen; Ispas, Diana; Pokar, Gero; Soetebier, Sven; Tutsch, Rainer

    2002-10-01

    Recent advances in the fields of MEMS and MOEMS often require precise assembly of very small parts with an accuracy of a few microns. In order to meet this demand, a new approach using a robot based on parallel mechanisms in combination with a novel 3D-vision system has been chosen. The planar parallel robot structure with 2 DOF provides a high resolution in the XY-plane. It carries two additional serial axes for linear and rotational movement in/about z direction. In order to achieve high precision as well as good dynamic capabilities, the drive concept for the parallel (main) axes incorporates air bearings in combination with a linear electric servo motors. High accuracy position feedback is provided by optical encoders with a resolution of 0.1 μm. To allow for visualization and visual control of assembly processes, a camera module fits into the hollow tool head. It consists of a miniature CCD camera and a light source. In addition a modular gripper support is integrated into the tool head. To increase the accuracy a control loop based on an optoelectronic sensor will be implemented. As a result of an in-depth analysis of different approaches a photogrammetric system using one single camera and special beam-splitting optics was chosen. A pattern of elliptical marks is applied to the surfaces of workpiece and gripper. Using a model-based recognition algorithm the image processing software identifies the gripper and the workpiece and determines their relative position. A deviation vector is calculated and fed into the robot control to guide the gripper.

  6. Evaluation of AN Integrated Gis-Based Crime Analysis & 3d Modelling for Izmir-Konak Municipality

    NASA Astrophysics Data System (ADS)

    Tarhan, C.; Deniz, D.

    2011-08-01

    GIS integrated 3D modelling is crucial for the city planning and design processes because urban modelling is a tool used in virtual environments, and provides convenience to work. Although, the creation and display of 3D city models for large regions is difficult it is vital for planning and designing safer cities, as well as public places. Today, crime is a significant problem in Turkey. When it was compared by years of crime rates, population growth and urbanization rate, an increasing more than in parallel has been observed. This paper aims to discuss GIS integrated 3D modelling affects in urban planning and design, explaining Turkish planning processes with GIS and 3D modelling. To do that, it presents a case study for Izmir Konak Municipality about GIS integrated crime analysis and 3D models of the crime scenes. Izmir crime records has been obtained from Izmir Police Department belonging to 2003-2004 and 2005 (D. Deniz, 2007) are used for districts' crime map. In the light of these data, the highest rate crime district, Konak, is analyzed between 2001 and 2005 data.

  7. An integrated system for 3D hip joint reconstruction from 2D X-rays: a preliminary validation study.

    PubMed

    Schumann, Steffen; Liu, Li; Tannast, Moritz; Bergmann, Mathias; Nolte, Lutz-P; Zheng, Guoyan

    2013-10-01

    The acquisition of conventional X-ray radiographs remains the standard imaging procedure for the diagnosis of hip-related problems. However, recent studies demonstrated the benefit of using three-dimensional (3D) surface models in the clinical routine. 3D surface models of the hip joint are useful for assessing the dynamic range of motion in order to identify possible pathologies such as femoroacetabular impingement. In this paper, we present an integrated system which consists of X-ray radiograph calibration and subsequent 2D/3D hip joint reconstruction for diagnosis and planning of hip-related problems. A mobile phantom with two different sizes of fiducials was developed for X-ray radiograph calibration, which can be robustly detected within the images. On the basis of the calibrated X-ray images, a 3D reconstruction method of the acetabulum was developed and applied together with existing techniques to reconstruct a 3D surface model of the hip joint. X-ray radiographs of dry cadaveric hip bones and one cadaveric specimen with soft tissue were used to prove the robustness of the developed fiducial detection algorithm. Computed tomography scans of the cadaveric bones were used to validate the accuracy of the integrated system. The fiducial detection sensitivity was in the same range for both sizes of fiducials. While the detection sensitivity was 97.96% for the large fiducials, it was 97.62% for the small fiducials. The acetabulum and the proximal femur were reconstructed with a mean surface distance error of 1.06 and 1.01 mm, respectively. The results for fiducial detection sensitivity and 3D surface reconstruction demonstrated the capability of the integrated system for 3D hip joint reconstruction from 2D calibrated X-ray radiographs.

  8. An integral equation approach to smooth 3D Navier-Stokes solution

    NASA Astrophysics Data System (ADS)

    Costin, O.; Luo, G.; Tanveer, S.

    2008-12-01

    We summarize a recently developed integral equation (IE) approach to tackling the long-time existence problem for smooth solution v(x, t) to the 3D Navier-Stokes (NS) equation in the context of a periodic box problem with smooth time independent forcing and initial condition v0. Using an inverse-Laplace transform of {\\skew5\\hat v} (k, t) - {\\skew5\\hat v}_0 in 1/t, we arrive at an IE for {\\skew5\\hat U} (k, p) , where p is inverse-Laplace dual to 1/t and k is the Fourier variable dual to x. The advantage of this formulation is that the solution {\\skew5\\hat U} to the IE is known to exist a priori for p \\in \\mathbb{R}^+ and the solution is integrable and exponentially bounded at ∞. Global existence of NS solution in this formulation is reduced to an asymptotics question. If \\parallel\\!{\\skew5\\hat U} (\\cdot, p)\\!\\parallel_{{l^{1} (\\mathbb{Z}^3)}} has subexponential bounds as p→∞, then global existence to NS follows. Moreover, if f=0, then the converse is also true in the following sense: if NS has global solution, then there exists n>=1 for which the inverse-Laplace transform of {\\skew5\\hat v} (k, t) - {\\skew5\\hat v}_0 in 1/tn necessarily decays as q→∞, where q is the inverse-Laplace dual to 1/tn. We also present refined estimates of the exponential growth when the solution {\\skew5\\hat U} is known on a finite interval [0, p0]. We also show that for analytic v[0] and f, with finitely many nonzero Fourier-coefficients, the series for {\\skew5\\hat U} (k, p) in powers of p has a radius of convergence independent of initial condition and forcing; indeed the radius gets bigger for smaller viscosity. We also show that the IE can be solved numerically with controlled errors. Preliminary numerical calculations for Kida (1985 J. Phys. Soc. Japan 54 2132) initial conditions, though far from being optimized, and performed on a modest interval in the accelerated variable q show decay in q.

  9. Integrating genomic information with protein sequence and 3D atomic level structure at the RCSB protein data bank

    PubMed Central

    Prlić, Andreas; Kalro, Tara; Bhattacharya, Roshni; Christie, Cole; Burley, Stephen K.; Rose, Peter W.

    2016-01-01

    Summary: The Protein Data Bank (PDB) now contains more than 120,000 three-dimensional (3D) structures of biological macromolecules. To allow an interpretation of how PDB data relates to other publicly available annotations, we developed a novel data integration platform that maps 3D structural information across various datasets. This integration bridges from the human genome across protein sequence to 3D structure space. We developed novel software solutions for data management and visualization, while incorporating new libraries for web-based visualization using SVG graphics. Availability and Implementation: The new views are available from http://www.rcsb.org and software is available from https://github.com/rcsb/. Contact: andreas.prlic@rcsb.org Supplementary information: Supplementary data are available at Bioinformatics online. PMID:27551105

  10. Estimation of uncertainties in geological 3D raster layer models as integral part of modelling procedures

    NASA Astrophysics Data System (ADS)

    Maljers, Denise; den Dulk, Maryke; ten Veen, Johan; Hummelman, Jan; Gunnink, Jan; van Gessel, Serge

    2016-04-01

    applied for DGM Deep proves to be an effective way to (graphically) represent the reliability of the DGM Deep model, although the relative contribution of the various error sources needs further attention. For the DGM Shallow model a cross-validation procedure in a moving window environment has been used to calculate mean deviations and standard errors on a sub-regional scale. Subsequently, these cross validation standard errors have been rescaled to account for local data configuration and clustering. This resulted in standard deviations expressing both regional and local uncertainties. Both workflows are state-of-the-art, form an integral part of the geological modelling and result in reproducible uncertainty values. They can be considered a good starting point for incorporating other errors that contribute to uncertainties of geological 3D raster layer models. For example, the mis-positioning of data used or the error underlying mis-ties at well locations. An additional, perhaps more easy-to-read, parameter that can be calculated to visualize these uncertainties would be the information entropy, as proposed by Wellmann & Regenauer-Lieb (2012). Where a value of 0 means there is no uncertainty, and a value of 1 means there is a high uncertainty. At the moment depth uncertainty information is disseminated through our webportals (www.dinoloket.nl and www.nlog.nl) in an on-line map viewer and as downloadable GIS products.

  11. Multi-Scale Modeling of an Integrated 3D Braided Composite with Applications to Helicopter Arm

    NASA Astrophysics Data System (ADS)

    Zhang, Diantang; Chen, Li; Sun, Ying; Zhang, Yifan; Qian, Kun

    2017-01-01

    A study is conducted with the aim of developing multi-scale analytical method for designing the composite helicopter arm with three-dimensional (3D) five-directional braided structure. Based on the analysis of 3D braided microstructure, the multi-scale finite element modeling is developed. Finite element analysis on the load capacity of 3D five-directional braided composites helicopter arm is carried out using the software ABAQUS/Standard. The influences of the braiding angle and loading condition on the stress and strain distribution of the helicopter arm are simulated. The results show that the proposed multi-scale method is capable of accurately predicting the mechanical properties of 3D braided composites, validated by the comparison the stress-strain curves of meso-scale RVCs. Furthermore, it is found that the braiding angle is an important factor affecting the mechanical properties of 3D five-directional braided composite helicopter arm. Based on the optimized structure parameters, the nearly net-shaped composite helicopter arm is fabricated using a novel resin transfer mould (RTM) process.

  12. Comparing and visualizing titanium implant integration in rat bone using 2D and 3D techniques.

    PubMed

    Arvidsson, Anna; Sarve, Hamid; Johansson, Carina B

    2015-01-01

    The aim was to compare the osseointegration of grit-blasted implants with and without a hydrogen fluoride treatment in rat tibia and femur, and to visualize bone formation using state-of-the-art 3D visualization techniques. Grit-blasted implants were inserted in femur and tibia of 10 Sprague-Dawley rats (4 implants/rat). Four weeks after insertion, bone implant samples were retrieved. Selected samples were imaged in 3D using Synchrotron Radiation-based μCT (SRμCT). The 3D data was quantified and visualized using two novel visualization techniques, thread fly-through and 2D unfolding. All samples were processed to cut and ground sections and 2D histomorphometrical comparisons of bone implant contact (BIC), bone area (BA), and mirror image area (MI) were performed. BA values were statistically significantly higher for test implants than controls (p < 0.05), but BIC and MI data did not differ significantly. Thus, the results partly indicate improved bone formation at blasted and hydrogen fluoride treated implants, compared to blasted implants. The 3D analysis was a valuable complement to 2D analysis, facilitating improved visualization. However, further studies are required to evaluate aspects of 3D quantitative techniques, with relation to light microscopy that traditionally is used for osseointegration studies.

  13. An Alignment Method for the Integration of Underwater 3D Data Captured by a Stereovision System and an Acoustic Camera

    PubMed Central

    Lagudi, Antonio; Bianco, Gianfranco; Muzzupappa, Maurizio; Bruno, Fabio

    2016-01-01

    The integration of underwater 3D data captured by acoustic and optical systems is a promising technique in various applications such as mapping or vehicle navigation. It allows for compensating the drawbacks of the low resolution of acoustic sensors and the limitations of optical sensors in bad visibility conditions. Aligning these data is a challenging problem, as it is hard to make a point-to-point correspondence. This paper presents a multi-sensor registration for the automatic integration of 3D data acquired from a stereovision system and a 3D acoustic camera in close-range acquisition. An appropriate rig has been used in the laboratory tests to determine the relative position between the two sensor frames. The experimental results show that our alignment approach, based on the acquisition of a rig in several poses, can be adopted to estimate the rigid transformation between the two heterogeneous sensors. A first estimation of the unknown geometric transformation is obtained by a registration of the two 3D point clouds, but it ends up to be strongly affected by noise and data dispersion. A robust and optimal estimation is obtained by a statistical processing of the transformations computed for each pose. The effectiveness of the method has been demonstrated in this first experimentation of the proposed 3D opto-acoustic camera. PMID:27089344

  14. Integration of both dense wavelength-division multiplexing and coarse wavelength-division multiplexing demultiplexer on one photonic crystal chip

    NASA Astrophysics Data System (ADS)

    Tian, Huiping; Shen, Guansheng; Liu, Weijia; Ji, Yuefeng

    2013-07-01

    An integrated model of photonic crystal (PC) demultiplexer that can be used to combine dense wavelength-division multiplexing (DWDM) and coarse wavelength-division multiplexing (CWDM) systems is first proposed. By applying the PC demultiplexer, dense channel spacing 0.8 nm and coarse channel spacing 20 nm are obtained at the same time. The transmission can be improved to nearly 90%, and the crosstalk can be decreased to less than -18 dB by enlarging the width of the bus waveguide. The total size of the device is 21×42 μm2. Four channels on one side of the demultiplexer can achieve DWDM in the wavelength range between 1575 and 1578 nm, and the other four channels on the other side can achieve CWDM in the wavelength range between 1490 and 1565 nm, respectively. The demonstrated demultiplexer can be applied in the future CWDM and DWDM system, and the architecture costs can be significantly reduced.

  15. A novel 3D integrated platform for the high-resolution study of cell migration plasticity.

    PubMed

    Schneider, Julian; Bachmann, Tobias; Franco, Davide; Richner, Patrizia; Galliker, Patrick; Tiwari, Manish K; Ferrari, Aldo; Poulikakos, Dimos

    2013-08-01

    Understanding the mechanisms of interstitial cancer migration is of great scientific and medical interest. Creating 3D platforms, conducive to optical microscopy and mimicking the physical parameters (in plane and out of plane) involved in interstitial migration, is a major step forward in this direction. Here, a novel approach is used to directly print free-form, 3D micropores on basal scaffolds containing microgratings optimized for contact guidance. The platforms so formed are validated by monitoring cancer cell migration and micropore penetration with high-resolution optical microscopy. The shapes, sizes and deformability of the micropores are controllable, paving the way to decipher their role in interstitial migration.

  16. A 3D profile function suitable for integration of neutron time-of-flight single crystal diffraction peaks

    NASA Astrophysics Data System (ADS)

    Gutmann, Matthias J.

    2017-03-01

    A 3D profile function is presented suitable to integrate reflections arising in time-of-flight (TOF) single crystal neutron diffraction experiments. In order to account for the large asymmetry of the peak shape in the TOF direction, a 3D Gaussian ellipsoid in the pixel (x, z) and time-of-flight coordinates is convoluted with a rising and falling exponential along the time-of-flight direction. An analytic expression is derived, making it suitable for least-squares fitting. The application of this function in detector space or reciprocal space is straightforward.

  17. An Integrated RELAP5-3D and Multiphase CFD Code System Utilizing a Semi Implicit Coupling Technique

    SciTech Connect

    D.L. Aumiller; E.T. Tomlinson; W.L. Weaver

    2001-06-21

    An integrated code system consisting of RELAP5-3D and a multiphase CFD program has been created through the use of a generic semi-implicit coupling algorithm. Unlike previous CFD coupling work, this coupling scheme is numerically stable provided the material Courant limit is not violated in RELAP5-3D or at the coupling locations. The basis for the coupling scheme and details regarding the unique features associated with the application of this technique to a four-field CFD program are presented. Finally, the results of a verification problem are presented. The coupled code system is shown to yield accurate and numerically stable results.

  18. Integrated Data Capturing Requirements for 3d Semantic Modelling of Cultural Heritage: the Inception Protocol

    NASA Astrophysics Data System (ADS)

    Di Giulio, R.; Maietti, F.; Piaia, E.; Medici, M.; Ferrari, F.; Turillazzi, B.

    2017-02-01

    The generation of high quality 3D models can be still very time-consuming and expensive, and the outcome of digital reconstructions is frequently provided in formats that are not interoperable, and therefore cannot be easily accessed. This challenge is even more crucial for complex architectures and large heritage sites, which involve a large amount of data to be acquired, managed and enriched by metadata. In this framework, the ongoing EU funded project INCEPTION - Inclusive Cultural Heritage in Europe through 3D semantic modelling proposes a workflow aimed at the achievements of efficient 3D digitization methods, post-processing tools for an enriched semantic modelling, web-based solutions and applications to ensure a wide access to experts and non-experts. In order to face these challenges and to start solving the issue of the large amount of captured data and time-consuming processes in the production of 3D digital models, an Optimized Data Acquisition Protocol (DAP) has been set up. The purpose is to guide the processes of digitization of cultural heritage, respecting needs, requirements and specificities of cultural assets.

  19. Integrated 3D-printed reactionware for chemical synthesis and analysis

    NASA Astrophysics Data System (ADS)

    Symes, Mark D.; Kitson, Philip J.; Yan, Jun; Richmond, Craig J.; Cooper, Geoffrey J. T.; Bowman, Richard W.; Vilbrandt, Turlif; Cronin, Leroy

    2012-05-01

    Three-dimensional (3D) printing has the potential to transform science and technology by creating bespoke, low-cost appliances that previously required dedicated facilities to make. An attractive, but unexplored, application is to use a 3D printer to initiate chemical reactions by printing the reagents directly into a 3D reactionware matrix, and so put reactionware design, construction and operation under digital control. Here, using a low-cost 3D printer and open-source design software we produced reactionware for organic and inorganic synthesis, which included printed-in catalysts and other architectures with printed-in components for electrochemical and spectroscopic analysis. This enabled reactions to be monitored in situ so that different reactionware architectures could be screened for their efficacy for a given process, with a digital feedback mechanism for device optimization. Furthermore, solely by modifying reactionware architecture, reaction outcomes can be altered. Taken together, this approach constitutes a relatively cheap, automated and reconfigurable chemical discovery platform that makes techniques from chemical engineering accessible to typical synthetic laboratories.

  20. Ship-in-a-bottle integration by hybrid femtosecond laser technology for fabrication of true 3D biochips

    NASA Astrophysics Data System (ADS)

    Sima, Felix; Wu, Dong; Xu, Jian; Midorikawa, Katsumi; Sugioka, Koji

    2015-03-01

    We propose herein the "ship-in-a-bottle" integration of three-dimensional (3D) polymeric sinusoidal ridges inside photosensitive glass microfluidic channel by a hybrid subtractive - additive femtosecond laser processing method. It consists of Femtosecond Laser Assisted Wet Etching (FLAE) of a photosensitive Foturan glass followed by Two-Photon Polymerization (TPP) of a SU-8 negative epoxy-resin. Both subtractive and additive processes are carried out using the same set-up with the change of laser focusing objective only. A 522 nm wavelength of the second harmonic generation from an amplified femtosecond Yb-fiber laser (FCPA µJewel D-400, IMRA America, 1045 nm; pulse width 360 fs, repetition rate 200 kHz) was employed for irradiation. The new method allows lowering the size limit of 3D objects created inside channels to smaller details down to the dimensions of a cell, and improve the structure stability. Sinusoidal periodic patterns and ridges are of great use as base scaffolds for building up new structures on their top or for modulating cell migration, guidance and orientation while created interspaces can be exploited for microfluidic applications. The glass microchannel offers robustness and appropriate dynamic flow conditions for cellular studies while the integrated patterns are reducing the size of structure to the level of cells responsiveness. Taking advantage of the ability to directly fabricate 3D complex shapes, both glass channels and polymeric integrated patterns enable us to 3D spatially design biochips for specific applications.

  1. Automated and integrated mask generation from a CAD constructed 3D model.

    SciTech Connect

    Schiek, Richard Louis; Schmidt, Rodney Cannon

    2005-03-01

    We have developed and implemented a method which given a three-dimensional object can infer from topology the two-dimensional masks needed to produce that object with surface micromachining. This design tool calculates the two-dimensional mask set required to produce a given three-dimensional model by investigating the vertical topology to the model. The 3D model is first separated into bodies that are non-intersecting, made from different materials or only linked through a ground plane. Next, for each body unique horizontal cross sections are located and arranged into a tree based on their topological relationship. A branch-wise search of the tree uncovers locations where deposition boundaries must lie and identifies candidate masks creating a generic mask set for the 3D model. Finally, in the last step specific process requirements are considered that may constrain the generic mask set.

  2. Integrating biologically inspired nanomaterials and table-top stereolithography for 3D printed biomimetic osteochondral scaffolds

    NASA Astrophysics Data System (ADS)

    Castro, Nathan J.; O'Brien, Joseph; Zhang, Lijie Grace

    2015-08-01

    The osteochondral interface of an arthritic joint is notoriously difficult to regenerate due to its extremely poor regenerative capacity and complex stratified architecture. Native osteochondral tissue extracellular matrix is composed of numerous nanoscale organic and inorganic constituents. Although various tissue engineering strategies exist in addressing osteochondral defects, limitations persist with regards to tissue scaffolding which exhibit biomimetic cues at the nano to micro scale. In an effort to address this, the current work focused on 3D printing biomimetic nanocomposite scaffolds for improved osteochondral tissue regeneration. For this purpose, two biologically-inspired nanomaterials have been synthesized consisting of (1) osteoconductive nanocrystalline hydroxyapatite (nHA) (primary inorganic component of bone) and (2) core-shell poly(lactic-co-glycolic) acid (PLGA) nanospheres encapsulated with chondrogenic transforming growth-factor β1 (TGF-β1) for sustained delivery. Then, a novel table-top stereolithography 3D printer and the nano-ink (i.e., nHA + nanosphere + hydrogel) were employed to fabricate a porous and highly interconnected osteochondral scaffold with hierarchical nano-to-micro structure and spatiotemporal bioactive factor gradients. Our results showed that human bone marrow-derived mesenchymal stem cell adhesion, proliferation, and osteochondral differentiation were greatly improved in the biomimetic graded 3D printed osteochondral construct in vitro. The current work served to illustrate the efficacy of the nano-ink and current 3D printing technology for efficient fabrication of a novel nanocomposite hydrogel scaffold. In addition, tissue-specific growth factors illustrated a synergistic effect leading to increased cell adhesion and directed stem cell differentiation.

  3. Integrated WiFi/PDR/Smartphone Using an Unscented Kalman Filter Algorithm for 3D Indoor Localization.

    PubMed

    Chen, Guoliang; Meng, Xiaolin; Wang, Yunjia; Zhang, Yanzhe; Tian, Peng; Yang, Huachao

    2015-09-23

    Because of the high calculation cost and poor performance of a traditional planar map when dealing with complicated indoor geographic information, a WiFi fingerprint indoor positioning system cannot be widely employed on a smartphone platform. By making full use of the hardware sensors embedded in the smartphone, this study proposes an integrated approach to a three-dimensional (3D) indoor positioning system. First, an improved K-means clustering method is adopted to reduce the fingerprint database retrieval time and enhance positioning efficiency. Next, with the mobile phone's acceleration sensor, a new step counting method based on auto-correlation analysis is proposed to achieve cell phone inertial navigation positioning. Furthermore, the integration of WiFi positioning with Pedestrian Dead Reckoning (PDR) obtains higher positional accuracy with the help of the Unscented Kalman Filter algorithm. Finally, a hybrid 3D positioning system based on Unity 3D, which can carry out real-time positioning for targets in 3D scenes, is designed for the fluent operation of mobile terminals.

  4. Integrated WiFi/PDR/Smartphone Using an Unscented Kalman Filter Algorithm for 3D Indoor Localization

    PubMed Central

    Chen, Guoliang; Meng, Xiaolin; Wang, Yunjia; Zhang, Yanzhe; Tian, Peng; Yang, Huachao

    2015-01-01

    Because of the high calculation cost and poor performance of a traditional planar map when dealing with complicated indoor geographic information, a WiFi fingerprint indoor positioning system cannot be widely employed on a smartphone platform. By making full use of the hardware sensors embedded in the smartphone, this study proposes an integrated approach to a three-dimensional (3D) indoor positioning system. First, an improved K-means clustering method is adopted to reduce the fingerprint database retrieval time and enhance positioning efficiency. Next, with the mobile phone’s acceleration sensor, a new step counting method based on auto-correlation analysis is proposed to achieve cell phone inertial navigation positioning. Furthermore, the integration of WiFi positioning with Pedestrian Dead Reckoning (PDR) obtains higher positional accuracy with the help of the Unscented Kalman Filter algorithm. Finally, a hybrid 3D positioning system based on Unity 3D, which can carry out real-time positioning for targets in 3D scenes, is designed for the fluent operation of mobile terminals. PMID:26404314

  5. 3D integration of microfluidics and microoptics inside photosensitive glass by femtosecond laser direct writing for photonic biosensing

    NASA Astrophysics Data System (ADS)

    Sugioka, Koji; Wang, Zhongke; Midorikawa, Katsumi

    2008-02-01

    Optical waveguides with a propagation loss of around 0.5 dB/cm are written inside photosensitive Foturan glass by internal modification of refractive index using femtosecond (fs) laser. Integration of the optical wafveguides with a micromirror enables us to bend the guided laser beam at an angle of 90° with a bending loss of less than 0.3 dB. In the meanwhile, a plano-convex microlens is completely embedded inside the Foturan glass chip via formation of a three-dimensional (3D) hollow microstructure using fs laser direct writing followed by heat treatment and successive wet etching. This technique can also be used to fabricate microfluidic devices and therefore realizes 3D integration of microoptical and microfluidic components by one continuous procedure. Subsequently, microoptical waveguides are further integrated into the single glass chip. Demonstration of optical measurements using the integrated microchip reveals that photonic biosensing can be performed with an efficiency increased by a factor of 8 for fluorescence detection and by a factor of 3 for absorption detection.

  6. New 3D parallel GILD electromagnetic modeling and nonlinear inversion using global magnetic integral and local differential equation

    SciTech Connect

    Xie, G.; Li, J.; Majer, E.; Zuo, D.

    1998-07-01

    This paper describes a new 3D parallel GILD electromagnetic (EM) modeling and nonlinear inversion algorithm. The algorithm consists of: (a) a new magnetic integral equation instead of the electric integral equation to solve the electromagnetic forward modeling and inverse problem; (b) a collocation finite element method for solving the magnetic integral and a Galerkin finite element method for the magnetic differential equations; (c) a nonlinear regularizing optimization method to make the inversion stable and of high resolution; and (d) a new parallel 3D modeling and inversion using a global integral and local differential domain decomposition technique (GILD). The new 3D nonlinear electromagnetic inversion has been tested with synthetic data and field data. The authors obtained very good imaging for the synthetic data and reasonable subsurface EM imaging for the field data. The parallel algorithm has high parallel efficiency over 90% and can be a parallel solver for elliptic, parabolic, and hyperbolic modeling and inversion. The parallel GILD algorithm can be extended to develop a high resolution and large scale seismic and hydrology modeling and inversion in the massively parallel computer.

  7. Integrated Photonic Orbital Angular Momentum Multiplexing and Demultiplexing on Chip

    DTIC Science & Technology

    2014-10-31

    with 379,960 bits tested. OCIS codes: (060.1810) Buffers , couplers , routers, switches and multiplexers; (050.4865) Optical vortices; (060.4230...Fabrication .................. 74 II.B.13. Multilayer Silicon Nitride Waveguide Coupler Design .................................................. 77...and demultiplexed by a circular arrangement of apertures, length-matched waveguides and a star coupler

  8. Europeana and 3D

    NASA Astrophysics Data System (ADS)

    Pletinckx, D.

    2011-09-01

    The current 3D hype creates a lot of interest in 3D. People go to 3D movies, but are we ready to use 3D in our homes, in our offices, in our communication? Are we ready to deliver real 3D to a general public and use interactive 3D in a meaningful way to enjoy, learn, communicate? The CARARE project is realising this for the moment in the domain of monuments and archaeology, so that real 3D of archaeological sites and European monuments will be available to the general public by 2012. There are several aspects to this endeavour. First of all is the technical aspect of flawlessly delivering 3D content over all platforms and operating systems, without installing software. We have currently a working solution in PDF, but HTML5 will probably be the future. Secondly, there is still little knowledge on how to create 3D learning objects, 3D tourist information or 3D scholarly communication. We are still in a prototype phase when it comes to integrate 3D objects in physical or virtual museums. Nevertheless, Europeana has a tremendous potential as a multi-facetted virtual museum. Finally, 3D has a large potential to act as a hub of information, linking to related 2D imagery, texts, video, sound. We describe how to create such rich, explorable 3D objects that can be used intuitively by the generic Europeana user and what metadata is needed to support the semantic linking.

  9. Integration Of 3D Geographic Information System (GIS) For Effective Waste Management Practice

    SciTech Connect

    Rood, G.J.; Hecox, G.R.

    2006-07-01

    Soil remediation in response to the presence of residual radioactivity resulting from past MED/AEC activities is currently in progress under the Formerly Utilized Sites Remedial Action Program near the St. Louis, MO airport. During GY05, approximately 92,000 cubic meters (120,000 cubic yards) of radioactive soil was excavated, packaged and transported via rail for disposal at U.S. Ecology or Envirocare of Utah, LLC. To facilitate the management of excavation/transportation/disposal activities, a 3D GIS was developed for the site that was used to estimate the in-situ radionuclide activities, activities in excavation block areas, and shipping activities using a sum-of ratio (SOR) method for combining various radionuclide compounds into applicable transportation and disposal SOR values. The 3D GIS was developed starting with the SOR values for the approximately 900 samples from 90 borings. These values were processed into a three-dimensional (3D) point grid using kriging with nominal grid spacing of 1.5 by 1.5 meter horizontal by 0.3 meter vertical. The final grid, clipped to the area and soil interval above the planned base of excavation, consisted of 210,000 individual points. Standard GIS volumetric and spatial join procedures were used to calculate the volume of soil represented by each grid point, the base of excavation, depth below ground surface, elevation, surface elevation and SOR values for each point in the final grid. To create the maps needed for management, the point grid results were spatially joined to each excavation area in 0.9 meter (3 foot) depth intervals and the average SOR and total volumes were calculations. The final maps were color-coded for easy identification of areas above the specific transportation or disposal criteria. (authors)

  10. Fabrication and characterization of gels with integrated channels using 3D printing with microfluidic nozzle for tissue engineering applications.

    PubMed

    Attalla, R; Ling, C; Selvaganapathy, P

    2016-02-01

    The lack of a simple and effective method to integrate vascular network with engineered scaffolds and tissue constructs remains one of the biggest challenges in true 3D tissue engineering. Here, we detail the use of a commercially available, low-cost, open-source 3D printer modified with a microfluidic print-head in order to develop a method for the generation of instantly perfusable vascular network integrated with gel scaffolds seeded with cells. The print-head features an integrated coaxial nozzle that allows the fabrication of hollow, calcium-polymerized alginate tubes that can be easily patterned using 3D printing techniques. The diameter of the hollow channel can be precisely controlled and varied between 500 μm - 2 mm by changing applied flow rates or print-head speed. These channels are integrated into gel layers with a thickness of 800 μm - 2.5 mm. The structural rigidity of these constructs allows the fabrication of multi-layered structures without causing the collapse of hollow channels in lower layers. The 3D printing method was fully characterized at a range of operating speeds (0-40 m/min) and corresponding flow rates (1-30 mL/min) were identified to produce precise definition. This microfluidic design also allows the incorporation of a wide range of scaffold materials as well as biological constituents such as cells, growth factors, and ECM material. Media perfusion of the channels causes a significant viability increase in the bulk of cell-laden structures over the long-term. With this setup, gel constructs with embedded arrays of hollow channels can be created and used as a potential substitute for blood vessel networks.

  11. The SVX3D integrated circuit for dead-timeless silicon strip readout

    NASA Astrophysics Data System (ADS)

    Garcia-Sciveres, M.; Milgrome, O.; Zimmerman, T.; Volobouev, I.; Ely, R. P.; Connolly, A.; Fish, D.; Affolder, T.; Sill, A.

    1999-10-01

    The revision D of the SVX3 readout IC has been fabricated in the Honeywell radiation-hard 0.8 μm bulk CMOS process, for instrumenting 712,704 silicon strips in the upgrade to the Collider Detector at Fermilab. This final revision incorporates new features and changes to the original architecture that were added to meet the goal of dead-timeless operation. This paper describes the features central to dead-timeless operation, and presents test data for un-irradiated and irradiated SVX3D chips.

  12. Integration of 3D and 2D imaging data for assured navigation in unknown environments: initial steps

    NASA Astrophysics Data System (ADS)

    Dill, Evan; Uijt de Haag, Maarten

    2009-05-01

    This paper discusses the initial steps of the development of a novel navigation method that integrates three-dimensional (3D) point cloud data, two-dimensional (2D) gray-level (intensity), and data from an Inertial Measurement Unit (IMU). A time-of-flight camera such as MESA's Swissranger will output both the 3D and 2D data. The target application is position and attitude determination of unmanned aerial vehicles (UAV) and autonomous ground vehicles (AGV) in urban or indoor environments. In urban and indoor environments a GPS position capability may not only be unavailable due to shadowing, significant signal attenuation or multipath, but also due to intentional denial or deception. The proposed algorithm extracts key features such as planar surfaces, lines and corner-points from both the 3D (point-cloud) and 2D (intensity) imagery. Consecutive observations of corresponding features in the 3D and 2D image frames are then used to compute estimates of position and orientation changes. Since the use of 3D image features for positioning suffers from limited feature observability resulting in deteriorated position accuracies, and the 2D imagery suffers from an unknown depth when estimating the pose from consecutive image frames, it is expected that the integration of both data sets will alleviate the problems with the individual methods resulting in an position and attitude determination method with a high level of assurance. An Inertial Measurement Unit (IMU) is used to set up the tracking gates necessary to perform data association of the features in consecutive frames. Finally, the position and orientation change estimates can be used to correct for the IMU drift errors.

  13. A line integration method for the treatment of 3D domain integrals and accelerated by the fast multipole method in the BEM

    NASA Astrophysics Data System (ADS)

    Wang, Qiao; Zhou, Wei; Cheng, Yonggang; Ma, Gang; Chang, Xiaolin

    2017-04-01

    A line integration method (LIM) is proposed to calculate the domain integrals for 3D problems. In the proposed method, the domain integrals are transformed into boundary integrals and only line integrals on straight lines are needed to be computed. A background cell structure is applied to further simplify the line integrals and improve the accuracy. The method creates elements only on the boundary, and the integral lines are created from the boundary elements. The procedure is quite suitable for the boundary element method, and we have applied it to 3D situations. Directly applying the method is time-consuming since the complexity of the computational time is O( NM), where N and M are the numbers of nodes and lines, respectively. To overcome this problem, the fast multipole method is used with the LIM for large-scale computation. The numerical results show that the proposed method is efficient and accurate.

  14. Digital Reconstruction of AN Archaeological Site Based on the Integration of 3d Data and Historical Sources

    NASA Astrophysics Data System (ADS)

    Guidi, G.; Russo, M.; Angheleddu, D.

    2013-02-01

    The methodology proposed in this paper in based on an integrated approach for creating a 3D digital reconstruction of an archaeological site, using extensively the 3D documentation of the site in its current state, followed by an iterative interaction between archaeologists and digital modelers, leading to a progressive refinement of the reconstructive hypotheses. The starting point of the method is the reality-based model, which, together with ancient drawings and documents, is used for generating the first reconstructive step. Such rough approximation of a possible architectural structure can be annotated through archaeological considerations that has to be confronted with geometrical constraints, producing a reduction of the reconstructive hypotheses to a limited set, each one to be archaeologically evaluated. This refinement loop on the reconstructive choices is iterated until the result become convincing by both points of view, integrating in the best way all the available sources. The proposed method has been verified on the ruins of five temples in the My Son site, a wide archaeological area located in central Vietnam. The integration of 3D surveyed data and historical documentation has allowed to support a digital reconstruction of not existing architectures, developing their three-dimensional digital models step by step, from rough shapes to highly sophisticate virtual prototypes.

  15. Formulation and implementation of a high-order 3-D domain integral method for the extraction of energy release rates

    NASA Astrophysics Data System (ADS)

    Ozer, H.; Duarte, C. A.; Al-Qadi, I. L.

    2012-04-01

    This article presents a three dimensional (3-D) formulation and implementation of a high-order domain integral method for the computation of energy release rate. The method is derived using surface and domain formulations of the J-integral and the weighted residual method. The J-integral along 3-D crack fronts is approximated by high-order Legendre polynomials. The proposed implementation is tailored for the Generalized/eXtended Finite Element Method and can handle discontinuities arbitrarily located within a finite element mesh. The domain integral calculations are based on the same integration elements used for the computation of the stiffness matrix. Discontinuities of the integrands across crack surfaces and across computational element boundaries are fully accounted for. The proposed method is able to deliver smooth approximations and to capture the boundary layer behavior of the J-integral using tetrahedral meshes. Numerical simulations of mode-I and mixed mode benchmark fracture mechanics examples verify expected convergence rates for the computed energy release rates. The results are also in good agreement with other numerical solutions available in the literature.

  16. Research on Joint Parameter Inversion for an Integrated Underground Displacement 3D Measuring Sensor

    PubMed Central

    Shentu, Nanying; Qiu, Guohua; Li, Qing; Tong, Renyuan; Shentu, Nankai; Wang, Yanjie

    2015-01-01

    Underground displacement monitoring is a key means to monitor and evaluate geological disasters and geotechnical projects. There exist few practical instruments able to monitor subsurface horizontal and vertical displacements simultaneously due to monitoring invisibility and complexity. A novel underground displacement 3D measuring sensor had been proposed in our previous studies, and great efforts have been taken in the basic theoretical research of underground displacement sensing and measuring characteristics by virtue of modeling, simulation and experiments. This paper presents an innovative underground displacement joint inversion method by mixing a specific forward modeling approach with an approximate optimization inversion procedure. It can realize a joint inversion of underground horizontal displacement and vertical displacement for the proposed 3D sensor. Comparative studies have been conducted between the measured and inversed parameters of underground horizontal and vertical displacements under a variety of experimental and inverse conditions. The results showed that when experimentally measured horizontal displacements and vertical displacements are both varied within 0 ~ 30 mm, horizontal displacement and vertical displacement inversion discrepancies are generally less than 3 mm and 1 mm, respectively, under three kinds of simulated underground displacement monitoring circumstances. This implies that our proposed underground displacement joint inversion method is robust and efficient to predict the measuring values of underground horizontal and vertical displacements for the proposed sensor. PMID:25871714

  17. New optical four-quadrant phase detector integrated into a photogate array for small and precise 3D cameras

    NASA Astrophysics Data System (ADS)

    Schwarte, Rudolf; Xu, Zhanping; Heinol, Horst-Guenther; Olk, Joachim; Buxbaum, Bernd

    1997-03-01

    The photonic mixer device (PMD) is a new electro-optical mixing semiconductor device. Integrated into a line or an array it may contribute a significant improvement in developing an extremely fast, flexible, robust and low cost 3D-solid-state camera. Three dimensional (3D)-cameras are of dramatically increasing interest in industrial automation, especially for production integrated quality control, in- house navigation, etc. The type of 3D-camera here under consideration is based on the principle of time-of-flight respectively phase delay of surface reflected echoes of rf- modulated light. In contrast to 3D-laser radars there is no scanner required since the whole 3D-scene is illuminated simultaneously using intensity-modulated incoherent light, e.g. in the 10 to 1000 MHz range. The rf-modulated light reflected from the 3D-scene represents the total depth information within the local delay of the back scattered phase front. If this incoming wave front is again rf- modulated by a 2D-mixer within the whole receiving aperture we get a quasi-stationary rf-inference pattern respectively rf-interferogram which may be captured by means of a conventional CCD-camera. This procedure is called rf- modulation interferometry (RFMI). Corresponding to first simulative results the new PMD-array will be appropriate to the RFMI-procedure. Though looking like a modified CCD-array or CMOS-photodetector array it will be able to perform both, the pixelwise mixing process for phase delay respectively depth evaluation and the pixelwise light intensity acquisition for gray level or color evaluation. Further advantageous properties are achieved by means of a four- quadrant (4Q)-PMD array which operates as a balanced inphase/quadrature phase (I/Q)-mixer and will be able to capture the total 3D-scene information of several 100,000 voxels within the microsecond(s) - to ms-range.

  18. Tightly coupled low cost 3D RISS/GPS integration using a mixture particle filter for vehicular navigation.

    PubMed

    Georgy, Jacques; Noureldin, Aboelmagd

    2011-01-01

    Satellite navigation systems such as the global positioning system (GPS) are currently the most common technique used for land vehicle positioning. However, in GPS-denied environments, there is an interruption in the positioning information. Low-cost micro-electro mechanical system (MEMS)-based inertial sensors can be integrated with GPS and enhance the performance in denied GPS environments. The traditional technique for this integration problem is Kalman filtering (KF). Due to the inherent errors of low-cost MEMS inertial sensors and their large stochastic drifts, KF, with its linearized models, has limited capabilities in providing accurate positioning. Particle filtering (PF) was recently suggested as a nonlinear filtering technique to accommodate for arbitrary inertial sensor characteristics, motion dynamics and noise distributions. An enhanced version of PF called the Mixture PF is utilized in this study to perform tightly coupled integration of a three dimensional (3D) reduced inertial sensors system (RISS) with GPS. In this work, the RISS consists of one single-axis gyroscope and a two-axis accelerometer used together with the vehicle's odometer to obtain 3D navigation states. These sensors are then integrated with GPS in a tightly coupled scheme. In loosely-coupled integration, at least four satellites are needed to provide acceptable GPS position and velocity updates for the integration filter. The advantage of the tightly-coupled integration is that it can provide GPS measurement update(s) even when the number of visible satellites is three or lower, thereby improving the operation of the navigation system in environments with partial blockages by providing continuous aiding to the inertial sensors even during limited GPS satellite availability. To effectively exploit the capabilities of PF, advanced modeling for the stochastic drift of the vertically aligned gyroscope is used. In order to benefit from measurement updates for such drift, which are

  19. Tightly Coupled Low Cost 3D RISS/GPS Integration Using a Mixture Particle Filter for Vehicular Navigation

    PubMed Central

    Georgy, Jacques; Noureldin, Aboelmagd

    2011-01-01

    Satellite navigation systems such as the global positioning system (GPS) are currently the most common technique used for land vehicle positioning. However, in GPS-denied environments, there is an interruption in the positioning information. Low-cost micro-electro mechanical system (MEMS)-based inertial sensors can be integrated with GPS and enhance the performance in denied GPS environments. The traditional technique for this integration problem is Kalman filtering (KF). Due to the inherent errors of low-cost MEMS inertial sensors and their large stochastic drifts, KF, with its linearized models, has limited capabilities in providing accurate positioning. Particle filtering (PF) was recently suggested as a nonlinear filtering technique to accommodate for arbitrary inertial sensor characteristics, motion dynamics and noise distributions. An enhanced version of PF called the Mixture PF is utilized in this study to perform tightly coupled integration of a three dimensional (3D) reduced inertial sensors system (RISS) with GPS. In this work, the RISS consists of one single-axis gyroscope and a two-axis accelerometer used together with the vehicle’s odometer to obtain 3D navigation states. These sensors are then integrated with GPS in a tightly coupled scheme. In loosely-coupled integration, at least four satellites are needed to provide acceptable GPS position and velocity updates for the integration filter. The advantage of the tightly-coupled integration is that it can provide GPS measurement update(s) even when the number of visible satellites is three or lower, thereby improving the operation of the navigation system in environments with partial blockages by providing continuous aiding to the inertial sensors even during limited GPS satellite availability. To effectively exploit the capabilities of PF, advanced modeling for the stochastic drift of the vertically aligned gyroscope is used. In order to benefit from measurement updates for such drift, which are

  20. Integration of Petrophysical Methods and 3D Printing Technology to Replicate Reservoir Pore Systems

    NASA Astrophysics Data System (ADS)

    Ishutov, S.; Hasiuk, F.; Gray, J.; Harding, C.

    2014-12-01

    Pore-scale imaging and modeling are becoming routine geoscience techniques of reservoir analysis and simulation in oil and gas industry. Three-dimensional printing may facilitate the transformation of pore-space imagery into rock models, which can be compared to traditional laboratory methods and literature data. Although current methodologies for rapid rock modeling and printing obscure many details of grain geometry, computed tomography data is one route to refine pore networks and experimentally test hypotheses related to rock properties, such as porosity and permeability. This study uses three-dimensional printing as a novel way of interacting with x-ray computed tomography data from reservoir core plugs based on digital modeling of pore systems in coarse-grained sandstones and limestones. The advantages of using artificial rocks as a proxy are to better understand the contributions of pore system characteristics at various scales to petrophysical properties in oil and gas reservoirs. Pore radii of reservoir sandstones used in this study range from 1 to 100s of microns, whereas the pore radii for limestones vary from 0.01 to 10s of microns. The resolution of computed tomography imaging is ~10 microns; the resolution of 3D digital printing used in the study varies from 2.5 to 300 microns. For this technology to be useful, loss of pore network information must be minimized in the course of data acquisition, modeling, and production as well as verified against core-scale measurements. The ultimate goal of this study is to develop a reservoir rock "photocopier" that couples 3D scanning and modeling with 3D printing to reproduce a) petrophyscially accurate copies of reservoir pore systems and b) digitally modified pore systems for testing hypotheses about reservoir flow. By allowing us to build porous media with known properties (porosity, permeability, surface area), technology will also advance our understanding of the tools used to measure these quantities (e

  1. 3D Integrated geophysical-petrological modelling of the Iranian lithosphere

    NASA Astrophysics Data System (ADS)

    Mousavi, Naeim; Ardestani, Vahid E.; Ebbing, Jörg; Fullea, Javier

    2016-04-01

    The present-day Iranian Plateau is the result of complex tectonic processes associated with the Arabia-Eurasia Plate convergence at a lithospheric scale. In spite of previous mostly 2D geophysical studies, fundamental questions regarding the deep lithospheric and sub-lithospheric structure beneath Iran remain open. A robust 3D model of the thermochemical lithospheric structure in Iran is an important step toward a better understanding of the geological history and tectonic events in the area. Here, we apply a combined geophysical-petrological methodology (LitMod3D) to investigate the present-day thermal and compositional structure in the crust and upper mantle beneath the Arabia-Eurasia collision zone using a comprehensive variety of constraining data: elevation, surface heat flow, gravity potential fields, satellite gravity gradients, xenoliths and seismic tomography. Different mantle compositions were tested in our model based on local xenolith samples and global data base averages for different tectonothermal ages. A uniform mantle composition fails to explain the observed gravity field, gravity gradients and surface topography. A tectonically regionalized lithospheric mantle compositional model is able to explain all data sets including seismic tomography models. Our preliminary thermochemical lithospheric study constrains the depth to Moho discontinuity and intra crustal geometries including depth to sediments. We also determine the depth to Curie isotherm which is known as the base of magnetized crustal/uppermost mantle bodies. Discrepancies with respect to previous studies include mantle composition and the geometry of Moho and Lithosphere-Asthenosphere Boundary (LAB). Synthetic seismic Vs and Vp velocities match existing seismic tomography models in the area. In this study, depleted mantle compositions are modelled beneath cold and thick lithosphere in Arabian and Turan platforms. A more fertile mantle composition is found in collision zones. Based on our 3

  2. One-Step Fabrication of a Microfluidic Device with an Integrated Membrane and Embedded Reagents by Multimaterial 3D Printing.

    PubMed

    Li, Feng; Smejkal, Petr; Macdonald, Niall P; Guijt, Rosanne M; Breadmore, Michael C

    2017-04-05

    One of the largest impediments in the development of microfluidic-based smart sensing systems is the manufacturability of integrated, complex devices. Here we propose multimaterial 3D printing for the fabrication of such devices in a single step. A microfluidic device containing an integrated porous membrane and embedded liquid reagents was made by 3D printing and applied for the analysis of nitrate in soil. The manufacture of the integrated, sealed device was realized as a single print within 30 min. The body of the device was printed in transparent acrylonitrile butadiene styrene (ABS) and contained a 400 μm wide structure printed from a commercially available composite filament. The composite filament can be turned into a porous material through dissolution of a water-soluble material. Liquid reagents were integrated by briefly pausing the printing before resuming for sealing the device. The devices were evaluated by the determination of nitrate in a soil slurry containing zinc particles for the reduction of nitrate to nitrite using the Griess reagent. Using a consumer digital camera, the linear range of the detector response ranged from 0 to 60 ppm, covering the normal range of nitrate in soil. To ensure that the sealing of the reagent chamber is maintained, aqueous reagents should be avoided. When using the nonaqueous reagent, the multimaterial device containing the Griess reagent could be stored for over 4 days but increased the detection range to 100-500 ppm. Multimaterial 3D printing is a potentially new approach for the manufacture of microfluidic devices with multiple integrated functional components.

  3. Localization and visualization of excess chemical potential in statistical mechanical integral equation theory 3D-HNC-RISM.

    PubMed

    Du, Qi-Shi; Liu, Peng-Jun; Huang, Ri-Bo

    2008-02-01

    In this study the excess chemical potential of the integral equation theory, 3D-RISM-HNC [Q. Du, Q. Wei, J. Phys. Chem. B 107 (2003) 13463-13470], is visualized in three-dimensional form and localized at interaction sites of solute molecule. Taking the advantage of reference interaction site model (RISM), the calculation equations of chemical excess potential are reformulized according to the solute interaction sites s in molecular space. Consequently the solvation free energy is localized at every interaction site of solute molecule. For visualization of the 3D-RISM-HNC calculation results, the excess chemical potentials are described using radial and three-dimensional diagrams. It is found that the radial diagrams of the excess chemical potentials are more sensitive to the bridge functions than the radial diagrams of solvent site density distributions. The diagrams of average excess chemical potential provide useful information of solute-solvent electrostatic and van der Waals interactions. The local description of solvation free energy at active sites of solute in 3D-RISM-HNC may broaden the application scope of statistical mechanical integral equation theory in solution chemistry and life science.

  4. A 3-D skeleton model & SEMG approach for integrated neck and low back pain analysis test batteries.

    PubMed

    D'amico, M; D'amico, G; Frascarello, M; Paniccia, M; Roncoletta, P; Vallasciani, M

    2008-01-01

    Since several years our group is working on a project to merge into a full 3D reliable and detailed human skeleton representation various segmental biomechanical models presented in literature. The obtained 3D skeleton model is fully parametric and can be fitted to each subject anthropometric characteristics. A non-ionising approach based on 3D opto-electronic measurements of body landmarks labelled by passive markers has been chosen to build the 3D parametric biomechanical skeleton model. A special focus has been devoted to identify and model the spine with a correct degree of accuracy and reliability. In spine pain related pathologies is of major importance the evaluation of functional limitations associated. This requires to integrate morphological characteristics with information deriving from other measurements devices as force platform data, surface EMG, foot pressure maps. The aim of this study is to present a multi-factorial approach which integrates rachis morphological characteristics with full skeleton kinematic, dynamic and SEMG measurements to quantify spine function and mobility in particular for neck and low back pain. A set of clinical-biomechanical tests have been implemented. Static posture characteristics are first evaluated. After that, patient is asked to perform specific motion test batteries in order to fully measure the whole ROMs (spine angles ranges and spine shape modifications) for Axial rotations, forward-backward flexion-extension, lateral bendings per each spine functional units (Skull and neck, thoracic and lumbar districts). During forward bending also a digital Schober test is performed. Such data are correlated to simultaneous SEMG muscle activities recording to investigate motor co-ordination/dysfunction as well as the presence absence of flexion-relaxation phenomena associated to pain.

  5. Integration of camera and range sensors for 3D pose estimation in robot visual servoing

    NASA Astrophysics Data System (ADS)

    Hulls, Carol C. W.; Wilson, William J.

    1998-10-01

    Range-vision sensor systems can incorporate range images or single point measurements. Research incorporating point range measurements has focused on the area of map generation for mobile robots. These systems can utilize the fact that the objects sensed tend to be large and planar. The approach presented in this paper fuses information obtained from a point range measurement with visual information to produce estimates of the relative 3D position and orientation of a small, non-planar object with respect to a robot end- effector. The paper describes a real-time sensor fusion system for performing dynamic visual servoing using a camera and a point laser range sensor. The system is based upon the object model reference approach. This approach, which can be used to develop multi-sensor fusion systems that fuse dynamic sensor data from diverse sensors in real-time, uses a description of the object to be sensed in order to develop a combined observation-dependency sensor model. The range- vision sensor system is evaluated in terms of accuracy and robustness. The results show that the use of a range sensor significantly improves the system performance when there is poor or insufficient camera information. The system developed is suitable for visual servoing applications, particularly robot assembly operations.

  6. Integration of Libration Point Orbit Dynamics into a Universal 3-D Autonomous Formation Flying Algorithm

    NASA Technical Reports Server (NTRS)

    Folta, David; Bauer, Frank H. (Technical Monitor)

    2001-01-01

    The autonomous formation flying control algorithm developed by the Goddard Space Flight Center (GSFC) for the New Millennium Program (NMP) Earth Observing-1 (EO-1) mission is investigated for applicability to libration point orbit formations. In the EO-1 formation-flying algorithm, control is accomplished via linearization about a reference transfer orbit with a state transition matrix (STM) computed from state inputs. The effect of libration point orbit dynamics on this algorithm architecture is explored via computation of STMs using the flight proven code, a monodromy matrix developed from a N-body model of a libration orbit, and a standard STM developed from the gravitational and coriolis effects as measured at the libration point. A comparison of formation flying Delta-Vs calculated from these methods is made to a standard linear quadratic regulator (LQR) method. The universal 3-D approach is optimal in the sense that it can be accommodated as an open-loop or closed-loop control using only state information.

  7. Integrating eye tracking and motion sensor on mobile phone for interactive 3D display

    NASA Astrophysics Data System (ADS)

    Sun, Yu-Wei; Chiang, Chen-Kuo; Lai, Shang-Hong

    2013-09-01

    In this paper, we propose an eye tracking and gaze estimation system for mobile phone. We integrate an eye detector, cornereye center and iso-center to improve pupil detection. The optical flow information is used for eye tracking. We develop a robust eye tracking system that integrates eye detection and optical-flow based image tracking. In addition, we further incorporate the orientation sensor information from the mobile phone to improve the eye tracking for accurate gaze estimation. We demonstrate the accuracy of the proposed eye tracking and gaze estimation system through experiments on some public video sequences as well as videos acquired directly from mobile phone.

  8. The Integration of GPR, GIS, and GPS for 3D Soil Morphologic Models

    NASA Astrophysics Data System (ADS)

    Tischler, M.; Collins, M. E.

    2005-05-01

    Ground-Penetrating Radar (GPR) has become a useful and efficient instrument for gathering information about subsurface diagnostic horizons in Florida soils. Geographic Information Systems (GIS) are a popular and valuable tool for spatial data analysis of real world features in a digital environment. Ground-Penetrating Radar can be linked to GIS by using Global Positioning Systems (GPS). By combining GPR, GPS, and GIS technologies, a more detailed geophysical survey can be completed for an area of interest by integratinghydrologic, pedologic, and geologic data. Thus, the objectives of this research were to identify subsurface soil layers using GPR and their geographic position with a highly accurate GPS; to develop a procedure to import GPR data into a popular software package, such as ArcGIS, and; to create 3D subsurface models based on the imported GPR data. The site for this study was the Plant Science Research and Education Center in Marion County, Florida. The soils are characterized by Recent-Pleistocene-age sand over the clayey, marine deposited Plio-Miocene-age Hawthorn Formation which drapes the Eocene-age Ocala Limestone. Consequently, soils in the research area vary from deep quartz sands (Typic Quartzipsamments) to shallow outcrops of the Hawthorn Formation (Arenic Hapludalfs). A GPR survey was performed on a 160 m x 320 m grid to gather data for processing. Four subsurface models estimating the depth to argillic horizon were created using a variety of specialized GPR data filters and geostatistical data analyses. The models were compared with ground-truth points that measured the depth to argillic horizon to validate each model and calculate error metrics. These models may assist research station personnel to determine best management practices (including experimental plot placement, irrigation management, fertilizer treatment, and pesticide applications). In addition, the developed methodology exploits the potential of combining GPR and GIS.

  9. A Integrated Service Platform for Remote Sensing Image 3D Interpretation and Draughting based on HTML5

    NASA Astrophysics Data System (ADS)

    LIU, Yiping; XU, Qing; ZhANG, Heng; LV, Liang; LU, Wanjie; WANG, Dandi

    2016-11-01

    The purpose of this paper is to solve the problems of the traditional single system for interpretation and draughting such as inconsistent standards, single function, dependence on plug-ins, closed system and low integration level. On the basis of the comprehensive analysis of the target elements composition, map representation and similar system features, a 3D interpretation and draughting integrated service platform for multi-source, multi-scale and multi-resolution geospatial objects is established based on HTML5 and WebGL, which not only integrates object recognition, access, retrieval, three-dimensional display and test evaluation but also achieves collection, transfer, storage, refreshing and maintenance of data about Geospatial Objects and shows value in certain prospects and potential for growth.

  10. Bright 3D display, native and integrated on-chip or system-level

    NASA Astrophysics Data System (ADS)

    Ellwood, Sutherland C., Jr.

    2011-06-01

    Photonica, Inc. has pioneered the use of magneto-optics and hybrid technologies in visual display systems to create arrays addressing hi-speed, solid-state modulators up to 1K times faster that DMD/DLP, yielding high frame-rate and extremely high net native resolution allowing for full-duplication of right eye and left eye modulators at 1080p, DCI 2K, 4K and other specified resolution requirements. The technology enables high-transmission (brightness) per frame. In one version, each integrated image-engine assembly processes binocular frames simultaneously, employing simultaneous right eye/left eye channels, either polarization-based or "Infitec" color-band based channels, as well as pixel-vector based systems. In another version, a multi-chip, massively parallel signal-processing architecture integrates pixel-signal channels to yield simultaneous binocular frames. This may be combined with on-chip integration. Channels are integrated either through optics elements on-chip or through fiber network or both.

  11. Encrypting 2D/3D image using improved lensless integral imaging in Fresnel domain

    NASA Astrophysics Data System (ADS)

    Li, Xiao-Wei; Wang, Qiong-Hua; Kim, Seok-Tae; Lee, In-Kwon

    2016-12-01

    We propose a new image encryption technique, for the first time to our knowledge, combined Fresnel transform with the improved lensless integral imaging technique. In this work, before image encryption, the input image is first recorded into an elemental image array (EIA) by using the improved lensless integral imaging technique. The recorded EIA is encrypted into random noise by use of two phase masks located in the Fresnel domain. The positions of phase masks and operation wavelength, as well as the integral imaging system parameters are used as encryption keys that can ensure security. Compared with previous works, the main novelty of this proposed method resides in the fact that the elemental images possess distributed memory characteristic, which greatly improved the robustness of the image encryption algorithm. Meanwhile, the proposed pixel averaging algorithm can effectively address the overlapping problem existing in the computational integral imaging reconstruction process. Numerical simulations are presented to demonstrate the feasibility and effectiveness of the proposed method. Results also indicate the high robustness against data loss attacks.

  12. A medical application integrating remote 3D visualization tools to access picture archiving and communication system on mobile devices.

    PubMed

    He, Longjun; Ming, Xing; Liu, Qian

    2014-04-01

    With computing capability and display size growing, the mobile device has been used as a tool to help clinicians view patient information and medical images anywhere and anytime. However, for direct interactive 3D visualization, which plays an important role in radiological diagnosis, the mobile device cannot provide a satisfactory quality of experience for radiologists. This paper developed a medical system that can get medical images from the picture archiving and communication system on the mobile device over the wireless network. In the proposed application, the mobile device got patient information and medical images through a proxy server connecting to the PACS server. Meanwhile, the proxy server integrated a range of 3D visualization techniques, including maximum intensity projection, multi-planar reconstruction and direct volume rendering, to providing shape, brightness, depth and location information generated from the original sectional images for radiologists. Furthermore, an algorithm that changes remote render parameters automatically to adapt to the network status was employed to improve the quality of experience. Finally, performance issues regarding the remote 3D visualization of the medical images over the wireless network of the proposed application were also discussed. The results demonstrated that this proposed medical application could provide a smooth interactive experience in the WLAN and 3G networks.

  13. Dual-mode intracranial catheter integrating 3D ultrasound imaging and hyperthermia for neuro-oncology: feasibility study.

    PubMed

    Herickhoff, Carl D; Light, Edward D; Bing, Kristin F; Mukundan, Srinivasan; Grant, Gerald A; Wolf, Patrick D; Smith, Stephen W

    2009-04-01

    In this study, we investigated the feasibility of an intracranial catheter transducer with dual-mode capability of real-time 3D (RT3D) imaging and ultrasound hyperthermia, for application in the visualization and treatment of tumors in the brain. Feasibility is demonstrated in two ways: first by using a 50-element linear array transducer (17 mm x 3.1 mm aperture) operating at 4.4 MHz with our Volumetrics diagnostic scanner and custom, electrical impedance-matching circuits to achieve a temperature rise over 4 degrees C in excised pork muscle, and second, by designing and constructing a 12 Fr, integrated matrix and linear-array catheter transducer prototype for combined RT3D imaging and heating capability. This dual-mode catheter incorporated 153 matrix array elements and 11 linear array elements diced on a 0.2 mm pitch, with a total aperture size of 8.4 mm x 2.3 mm. This 3.64 MHz array achieved a 3.5 degrees C in vitro temperature rise at a 2 cm focal distance in tissue-mimicking material. The dual-mode catheter prototype was compared with a Siemens 10 Fr AcuNav catheter as a gold standard in experiments assessing image quality and therapeutic potential and both probes were used in an in vivo canine brain model to image anatomical structures and color Doppler blood flow and to attempt in vivo heating.

  14. The integration of 3D carbon-electrode dielectrophoresis on a CD-like centrifugal microfluidic platform.

    PubMed

    Martinez-Duarte, Rodrigo; Gorkin, Robert A; Abi-Samra, Kameel; Madou, Marc J

    2010-04-21

    We introduce the integration of a novel dielectrophoresis (DEP)-assisted filter with a compact disk (CD)-based centrifugal platform. Carbon-electrode dielectrophoresis (carbon-DEP) refers to the use of carbon electrodes to induce DEP. In this work, 3D carbon electrodes are fabricated using the C-MEMS technique and are used to implement a DEP-enabled active filter to trap particles of interest. Compared to traditional planar metal electrodes, 3D carbon electrodes allow for superior filtering efficiency. The system includes mounting modular 3D carbon-DEP chips on an electrically interfaced rotating disk. This allows simple centrifugal pumping to replace the large footprint syringe pump approaches commonly used in DEP systems. The advantages of the CD setup include not only a reduced footprint, but also complexity and cost reduction by eliminating expensive precision pumps and fluidic interconnects. To demonstrate the viability of this system we quantified the filter efficiency in the DEP trapping of yeast cells from a mix of latex and yeast cells. Results demonstrate selective filtering at flow rates up to 35 microl min(-1). The impact of electrode height, DEP chip misalignment and particle sedimentation on filter efficiency and the advantages this system represents are analyzed. The ultimate goal is to obtain an automated platform for bioparticle sorting with application in different fields such as point-of-care diagnostics and cell-based therapies.

  15. Architectural integration of the components necessary for electrical energy storage on the nanoscale and in 3D.

    PubMed

    Rhodes, Christopher P; Long, Jeffrey W; Pettigrew, Katherine A; Stroud, Rhonda M; Rolison, Debra R

    2011-04-01

    We describe fabrication of three-dimensional (3D) multifunctional nanoarchitectures in which the three critical components of a battery--cathode, separator/electrolyte, and anode--are internally assembled as tricontinuous nanoscopic phases. The architecture is initiated using sol-gel chemistry and processing to erect a 3D self-wired nanoparticulate scaffold of manganese oxide (>200 m(2) g(-1)) with a continuous, open, and mesoporous void volume. The integrated 3D system is generated by exhaustive coverage of the oxide network by an ultrathin, conformal layer of insulating polymer that forms via self-limiting electrodeposition of poly(phenylene oxide). The remaining interconnected void volume is then wired with RuO(2) nanowebs using subambient thermal decomposition of RuO(4). Transmission electron microscopy demonstrates that the three nanoscopic charge-transfer functional components--manganese oxide, polymer separator/cation conductor, and RuO(2)--exhibit the stratified, tricontinuous design of the phase-by-phase construction. This architecture contains all three components required for a solid-state energy storage device within a void volume sized at tens of nanometres such that nanometre-thick distances are established between the opposing electrodes. We have now demonstrated the ability to assemble multifunctional energy-storage nanoarchitectures on the nanoscale and in three dimensions.

  16. Integrated 3D geophysical and geological modelling of the Hercynian Suture Zone in the Champtoceaux area (south Brittany, France)

    NASA Astrophysics Data System (ADS)

    Martelet, G.; Calcagno, P.; Gumiaux, C.; Truffert, C.; Bitri, A.; Gapais, D.; Brun, J. P.

    2004-04-01

    This paper combines geological knowledge and geophysical imagery at the crustal scale to model the 3D geometry of a segment of the Hercynian suture zone of western Europe in the Champtoceaux area (Brittany, France). The Champtoceaux complex consists of a stack of metamorphic nappes of gneisses and micaschists, with eclogite-bearing units. The exhumation of the complex, during early Carboniferous times, was accompanied by deformation during regional dextral strike-slip associated with a major Hercynian shear zone (the South Armorican Shear Zone, SASZ). Dextral shearing produced a km-scale antiformal structure with a steeply dipping axial plane and a steeply eastward plunging axis. Armor 2 deep seismic profile shows that the regional structure was cut by a set of faults with northward thrusting components. Based on the seismic constraint, direct 2D crustal-scale modelling was performed throughout the Champtoceaux fold on seven radial gravity profiles, also using geological data, and density measurements from field and drill-hole samples. The 3D integration of the cross-sections, the digitised geological map, and the structural information (foliation dips) insure the geometrical and topological consistency of all sources of data. The 2D information is interpolated to the whole 3D space using a geostatistical analysis. Finally, the 3D gravity contribution of the resulting model is computed taking into account densities for each modelled geological body and compared to the Bouguer anomaly. The final 3D model is thus compatible with the seismic and gravity data, as well as with geological data. Main geological results derived from the modelling are (i) the overall 3D geometry of the south dipping thrust system interpreted on the seismic profile emphasises northward thrusting and folding of the Champtoceaux complex which was coeval with strike-slip along the South Armorican Shear Zone; (ii) the gravity modelling suggests the presence of a relatively dense body below the

  17. 3D-integrated optics component for astronomical spectro-interferometry.

    PubMed

    Saviauk, Allar; Minardi, Stefano; Dreisow, Felix; Nolte, Stefan; Pertsch, Thomas

    2013-07-01

    We present the experimental characterization of a spectro-interferometry setup based on a laser-written three-dimensional integrated optics component. By exploiting the interferometric capability of a two-dimensional array of evanescently coupled waveguides, we measure the mutual coherence properties of three different polychromatic optical fields. Direct application of our discrete beam combiner (DBC) component is astronomical interferometry. The DBC can be scaled up to combine arbitrary large number of telescopes for the determination of coherence properties of astronomical targets. Besides applications to astronomy, the DBC can be also applied to optical integrated metrology system requiring nanometric position monitoring. The working principle, the experimental setup used, and the broadband performance of the DBC are presented.

  18. 5D Data Modelling: Full Integration of 2D/3D Space, Time and Scale Dimensions

    NASA Astrophysics Data System (ADS)

    van Oosterom, Peter; Stoter, Jantien

    This paper proposes an approach for data modelling in five dimensions. Apart from three dimensions for geometrical representation and a fourth dimension for time, we identify scale as fifth dimensional characteristic. Considering scale as an extra dimension of geographic information, fully integrated with the other dimensions, is new. Through a formal definition of geographic data in a conceptual 5D continuum, the data can be handled by one integrated approach assuring consistency across scale and time dimensions. Because the approach is new and challenging, we choose to step-wise studying several combinations of the five dimensions, ultimately resulting in the optimal 5D model. We also propose to apply mathematical theories on multidimensional modelling to well established principles of multidimensional modelling in the geo-information domain. The result is a conceptual full partition of the 3Dspace+time+scale space (i.e. no overlaps, no gaps) realised in a 5D data model implemented in a Database Management System.

  19. Rapid Low-Temperature 3D Integration of Silicon Nanowires on Flexible Substrates.

    PubMed

    Kim, Yoonkap; Kim, Han-Jung; Kim, Jae-Hyun; Choi, Dae-Geun; Choi, Jun-Hyuk; Jung, Joo-Yun; Jeon, Sohee; Lee, Eung-Sug; Jeong, Jun-Ho; Lee, Jihye

    2015-08-26

    The vertical integration of 1D nanostructures onto the 2D substrates has the potential to offer significant performance gains to flexible electronic devices due to high integration density, large surface area, and improved light absorption and trapping. A simple, rapid, and low temperature transfer bonding method has been developed for this purpose. Ultrasonic vibration is used to achieve a low temperature bonding within a few seconds, resulting in a polymer-matrix-free, electrically conducting vertical assembly of silicon nanowires (SiNWs) with a graphene/PET substrate. The microscopic structure, and mechanical and electrical characteristics of the interface between the transferred SiNW array and graphene layer are subsequently investigated, revealing that this creates a mechanically robust and electrically Ohmic contact. This newly developed ultrasonic transfer bonding technique is also found to be readily adaptable for diverse substrates of both metal and polymer. It is therefore considered as a valuable technique for integrating 1D vertical nanostructures onto the 2D flexible substrates for flexible photovoltaics, energy storage, and water splitting systems.

  20. A near-infrared 64-pixel superconducting nanowire single photon detector array with integrated multiplexed readout

    SciTech Connect

    Allman, M. S. Verma, V. B.; Stevens, M.; Gerrits, T.; Horansky, R. D.; Lita, A. E.; Mirin, R.; Nam, S. W.; Marsili, F.; Beyer, A.; Shaw, M. D.; Kumor, D.

    2015-05-11

    We demonstrate a 64-pixel free-space-coupled array of superconducting nanowire single photon detectors optimized for high detection efficiency in the near-infrared range. An integrated, readily scalable, multiplexed readout scheme is employed to reduce the number of readout lines to 16. The cryogenic, optical, and electronic packaging to read out the array as well as characterization measurements are discussed.

  1. Conception d'un circuit d'etouffement pour photodiodes a avalanche en mode geiger pour integration heterogene 3d

    NASA Astrophysics Data System (ADS)

    Boisvert, Alexandre

    Le Groupe de Recherche en Appareillage Medical de Sherbrooke (GRAMS) travaille actuellement sur un programme de recherche portant sur des photodiodes a avalanche mono-photoniques (PAMP) operees en mode Geiger en vue d'une application a la tomographie d'emission par positrons (TEP). Pour operer dans ce mode; la PAMP, ou SPAD selon l'acronyme anglais (Single Photon Avalanche Diode), requiert un circuit d'etouffement (CE) pour, d'une part, arreter l'avalanche pouvant causer sa destruction et, d'autre part. la reinitialiser en mode d'attente d'un nouveau photon. Le role de ce CE comprend egalement une electronique de communication vers les etages de traitement avance de signaux. La performance temporelle optimale du CE est realisee lorsqu'il est juxtapose a la PAMP. Cependant, cela entraine une reduction de la surface photosensible ; un element crucial en imagerie. L'integration 3D, a base d'interconnexions verticales, offre une solution elegante et performante a cette problematique par l'empilement de circuits integres possedant differentes fonctions (PAMP, CE et traitement avance de signaux). Dans l'approche proposee, des circuits d'etouffement de 50 pm x 50 pm realises sur une technologie CMOS 130 mn 3D Tezzaron, contenant chacun 112 transistors, sont matrices afin de correspondre a une matrice de PAMP localisee sur une couche electronique superieure. Chaque circuit d'etouffement possede une gigue temporelle de 7,47 ps RMS selon des simulations faites avec le logiciel Cadence. Le CE a la flexibilite d'ajuster les temps d'etouffement et de recharge pour la PAMP tout en presentant une faible consommation de puissance (~ 0,33 mW a 33 Mcps). La conception du PAMP necessite de supporter des tensions superieures aux 3,3 V de la technologie. Pour repondre a ce probleme, des transistors a drain etendu (DEMOS) ont ete realises. En raison de retards de production par Ies fabricants, les circuits n'ont pu etre testes physiquement par des mesures. Les resultats de ce memoire

  2. Integrating Sensors into a Marine Drone for Bathymetric 3D Surveys in Shallow Waters.

    PubMed

    Giordano, Francesco; Mattei, Gaia; Parente, Claudio; Peluso, Francesco; Santamaria, Raffaele

    2015-12-29

    This paper demonstrates that accurate data concerning bathymetry as well as environmental conditions in shallow waters can be acquired using sensors that are integrated into the same marine vehicle. An open prototype of an unmanned surface vessel (USV) named MicroVeGA is described. The focus is on the main instruments installed on-board: a differential Global Position System (GPS) system and single beam echo sounder; inertial platform for attitude control; ultrasound obstacle-detection system with temperature control system; emerged and submerged video acquisition system. The results of two cases study are presented, both concerning areas (Sorrento Marina Grande and Marechiaro Harbour, both in the Gulf of Naples) characterized by a coastal physiography that impedes the execution of a bathymetric survey with traditional boats. In addition, those areas are critical because of the presence of submerged archaeological remains that produce rapid changes in depth values. The experiments confirm that the integration of the sensors improves the instruments' performance and survey accuracy.

  3. Integration of GIS, Geostatistics, and 3-D Technology to Assess the Spatial Distribution of Soil Moisture

    NASA Technical Reports Server (NTRS)

    Betts, M.; Tsegaye, T.; Tadesse, W.; Coleman, T. L.; Fahsi, A.

    1998-01-01

    The spatial and temporal distribution of near surface soil moisture is of fundamental importance to many physical, biological, biogeochemical, and hydrological processes. However, knowledge of these space-time dynamics and the processes which control them remains unclear. The integration of geographic information systems (GIS) and geostatistics together promise a simple mechanism to evaluate and display the spatial and temporal distribution of this vital hydrologic and physical variable. Therefore, this research demonstrates the use of geostatistics and GIS to predict and display soil moisture distribution under vegetated and non-vegetated plots. The research was conducted at the Winfred Thomas Agricultural Experiment Station (WTAES), Hazel Green, Alabama. Soil moisture measurement were done on a 10 by 10 m grid from tall fescue grass (GR), alfalfa (AA), bare rough (BR), and bare smooth (BS) plots. Results indicated that variance associated with soil moisture was higher for vegetated plots than non-vegetated plots. The presence of vegetation in general contributed to the spatial variability of soil moisture. Integration of geostatistics and GIS can improve the productivity of farm lands and the precision of farming.

  4. Integrating Sensors into a Marine Drone for Bathymetric 3D Surveys in Shallow Waters

    PubMed Central

    Giordano, Francesco; Mattei, Gaia; Parente, Claudio; Peluso, Francesco; Santamaria, Raffaele

    2015-01-01

    This paper demonstrates that accurate data concerning bathymetry as well as environmental conditions in shallow waters can be acquired using sensors that are integrated into the same marine vehicle. An open prototype of an unmanned surface vessel (USV) named MicroVeGA is described. The focus is on the main instruments installed on-board: a differential Global Position System (GPS) system and single beam echo sounder; inertial platform for attitude control; ultrasound obstacle-detection system with temperature control system; emerged and submerged video acquisition system. The results of two cases study are presented, both concerning areas (Sorrento Marina Grande and Marechiaro Harbour, both in the Gulf of Naples) characterized by a coastal physiography that impedes the execution of a bathymetric survey with traditional boats. In addition, those areas are critical because of the presence of submerged archaeological remains that produce rapid changes in depth values. The experiments confirm that the integration of the sensors improves the instruments’ performance and survey accuracy. PMID:26729117

  5. Uncertainty preserving patch-based online modeling for 3D model acquisition and integration from passive motion imagery

    NASA Astrophysics Data System (ADS)

    Tang, Hao; Chang, Peng; Molina, Edgardo; Zhu, Zhigang

    2012-06-01

    In both military and civilian applications, abundant data from diverse sources captured on airborne platforms are often available for a region attracting interest. Since the data often includes motion imagery streams collected from multiple platforms flying at different altitudes, with sensors of different field of views (FOVs), resolutions, frame rates and spectral bands, it is imperative that a cohesive site model encompassing all the information can be quickly built and presented to the analysts. In this paper, we propose to develop an Uncertainty Preserving Patch-based Online Modeling System (UPPOMS) leading towards the automatic creation and updating of a cohesive, geo-registered, uncertaintypreserving, efficient 3D site terrain model from passive imagery with varying field-of-views and phenomenologies. The proposed UPPOMS has the following technical thrusts that differentiate our approach from others: (1) An uncertaintypreserved, patch-based 3D model is generated, which enables the integration of images captured with a mixture of NFOV and WFOV and/or visible and infrared motion imagery sensors. (2) Patch-based stereo matching and multi-view 3D integration are utilized, which are suitable for scenes with many low texture regions, particularly in mid-wave infrared images. (3) In contrast to the conventional volumetric algorithms, whose computational and storage costs grow exponentially with the amount of input data and the scale of the scene, the proposed UPPOMS system employs an online algorithmic pipeline, and scales well to large amount of input data. Experimental results and discussions of future work will be provided.

  6. High-temperature compatible 3D-integration processes for a vacuum-sealed CNT-based NEMS

    NASA Astrophysics Data System (ADS)

    Gueye, R.; Lee, S. W.; Akiyama, T.; Briand, D.; Roman, C.; Hierold, C.; de Rooij, N. F.

    2013-03-01

    A System-in-Package (SiP) concept for the 3D-integration of a Single Wall Carbon Nanotube (SWCNT) resonator with its CMOS driving electronics is presented. The key element of this advanced SiP is the monolithic 3D-integration of the MEMS with the CMOS electronics using Through Silicon Vias (TSVs) on an SOI wafer. This SiP includes: A glass cap vacuum-sealed to the main wafer using an eutectic bonding process: a low leak rate of 2.7 10-9 mbar•l/s was obtained; Platinum-TSVs, compatible with the SWCNT growth and release process; The TSVs were developed in a "via first" process and characterized at high-temperature — up to 850 °C. An ohmic contact between the Pt-metallization and the SOI silicon device layer was obtained; The driving CMOS electronic device is assembled to the MEMS using an Au stud bump technology. Keywords: System-in-Package (SiP), vacuum packaging, eutectic bonding, "via-first" TSVs, high-temperature platinum interconnects, ohmic contacts, Au-stud bumps assembly, CMOS electronics.

  7. Low cost 3D-printing used in an undergraduate project: an integrating sphere for measurement of photoluminescence quantum yield

    NASA Astrophysics Data System (ADS)

    Tomes, John J.; Finlayson, Chris E.

    2016-09-01

    We report upon the exploitation of the latest 3D printing technologies to provide low-cost instrumentation solutions, for use in an undergraduate level final-year project. The project addresses prescient research issues in optoelectronics, which would otherwise be inaccessible to such undergraduate student projects. The experimental use of an integrating sphere in conjunction with a desktop spectrometer presents opportunities to use easily handled, low cost materials as a means to illustrate many areas of physics such as spectroscopy, lasers, optics, simple circuits, black body radiation and data gathering. Presented here is a 3rd year undergraduate physics project which developed a low cost (£25) method to manufacture an experimentally accurate integrating sphere by 3D printing. Details are given of both a homemade internal reflectance coating formulated from readily available materials, and a robust instrument calibration method using a tungsten bulb. The instrument is demonstrated to give accurate and reproducible experimental measurements of luminescence quantum yield of various semiconducting fluorophores, in excellent agreement with literature values.

  8. Classification of Informal Settlements Through the Integration of 2d and 3d Features Extracted from Uav Data

    NASA Astrophysics Data System (ADS)

    Gevaert, C. M.; Persello, C.; Sliuzas, R.; Vosselman, G.

    2016-06-01

    Unmanned Aerial Vehicles (UAVs) are capable of providing very high resolution and up-to-date information to support informal settlement upgrading projects. In order to provide accurate basemaps, urban scene understanding through the identification and classification of buildings and terrain is imperative. However, common characteristics of informal settlements such as small, irregular buildings with heterogeneous roof material and large presence of clutter challenge state-of-the-art algorithms. Especially the dense buildings and steeply sloped terrain cause difficulties in identifying elevated objects. This work investigates how 2D radiometric and textural features, 2.5D topographic features, and 3D geometric features obtained from UAV imagery can be integrated to obtain a high classification accuracy in challenging classification problems for the analysis of informal settlements. It compares the utility of pixel-based and segment-based features obtained from an orthomosaic and DSM with point-based and segment-based features extracted from the point cloud to classify an unplanned settlement in Kigali, Rwanda. Findings show that the integration of 2D and 3D features leads to higher classification accuracies.

  9. Fabrication of Large-Scale Microlens Arrays Based on Screen Printing for Integral Imaging 3D Display.

    PubMed

    Zhou, Xiongtu; Peng, Yuyan; Peng, Rong; Zeng, Xiangyao; Zhang, Yong-Ai; Guo, Tailiang

    2016-09-14

    The low-cost large-scale fabrication of microlens arrays (MLAs) with precise alignment, great uniformity of focusing, and good converging performance are of great importance for integral imaging 3D display. In this work, a simple and effective method for large-scale polymer microlens arrays using screen printing has been successfully presented. The results show that the MLAs possess high-quality surface morphology and excellent optical performances. Furthermore, the microlens' shape and size, i.e., the diameter, the height, and the distance between two adjacent microlenses of the MLAs can be easily controlled by modifying the reflowing time and the size of open apertures of the screen. MLAs with the neighboring microlenses almost tangent can be achieved under suitable size of open apertures of the screen and reflowing time, which can remarkably reduce the color moiré patterns caused by the stray light between the blank areas of the MLAs in the integral imaging 3D display system, exhibiting much better reconstruction performance.

  10. Guided wave-based J-integral estimation for dynamic stress intensity factors using 3D scanning laser Doppler vibrometry

    NASA Astrophysics Data System (ADS)

    Ayers, J.; Owens, C. T.; Liu, K. C.; Swenson, E.; Ghoshal, A.; Weiss, V.

    2013-01-01

    The application of guided waves to interrogate remote areas of structural components has been researched extensively in characterizing damage. However, there exists a sparsity of work in using piezoelectric transducer-generated guided waves as a method of assessing stress intensity factors (SIF). This quantitative information enables accurate estimation of the remaining life of metallic structures exhibiting cracks, such as military and commercial transport vehicles. The proposed full wavefield approach, based on 3D laser vibrometry and piezoelectric transducer-generated guided waves, provides a practical means for estimation of dynamic stress intensity factors (DSIF) through local strain energy mapping via the J-integral. Strain energies and traction vectors can be conveniently estimated from wavefield data recorded using 3D laser vibrometry, through interpolation and subsequent spatial differentiation of the response field. Upon estimation of the Jintegral, it is possible to obtain the corresponding DSIF terms. For this study, the experimental test matrix consists of aluminum plates with manufactured defects representing canonical elliptical crack geometries under uniaxial tension that are excited by surface mounted piezoelectric actuators. The defects' major to minor axes ratios vary from unity to approximately 133. Finite element simulations are compared to experimental results and the relative magnitudes of the J-integrals are examined.

  11. An integrated 3D constant offset GPR and resistivity survey on a sealed landfill — Ilhavo, NW Portugal

    NASA Astrophysics Data System (ADS)

    Hermozilha, H.; Grangeia, C.; Matias, M. Senos

    2010-01-01

    Owing to their nature landfills are challenging targets for high resolution Near Surface Geophysics. Herein it is described an integrated high resolution geophysical survey over the Ilhavo landfill sealed about a decade ago. The first aim of the survey is to investigate the time evolution of the contamination plume of the landfill since operations stopped and sealing took place. The second, and main objective, is the study of the landfill itself, that is, to carry out a high resolution 3D geophysical survey over it in order to investigate the thickness and effectiveness of the top cover, the thickness and sealing conditions of the landfill bottom and, finally, to investigate its internal structure. To fulfill these objectives an integrated 3D constant offset GPR and resistivity survey was designed. The interpretation of the geophysical data was carried out together with local borehole and hydrogeological information, so that, the ambiguity and uncertainty of the interpretation was reduced considerably and the usefulness of the methods were assessed.

  12. Monolithically mode division multiplexing photonic integrated circuit for large-capacity optical interconnection.

    PubMed

    Chen, Guanyu; Yu, Yu; Zhang, Xinliang

    2016-08-01

    We propose and fabricate an on-chip mode division multiplexed (MDM) photonic interconnection system. Such a monolithically photonic integrated circuit (PIC) is composed of a grating coupler, two micro-ring modulators, mode multiplexer/demultiplexer, and two germanium photodetectors. The signals' generation, multiplexing, transmission, demultiplexing, and detection are successfully demonstrated on the same chip. Twenty Gb/s MDM signals are successfully processed with clear and open eye diagrams, validating the feasibility of the proposed circuit. The measured power penalties show a good performance of the MDM link. The proposed on-chip MDM system can be potentially used for large-capacity optical interconnection in future high-performance computers and big data centers.

  13. Switchable in-line monitor for multi-dimensional multiplexed photonic integrated circuit.

    PubMed

    Chen, Guanyu; Yu, Yu; Ye, Mengyuan; Zhang, Xinliang

    2016-06-27

    A flexible monitor suitable for the discrimination of on-chip transmitted mode division multiplexed (MDM) and wavelength division multiplexed (WDM) signals is proposed and fabricated. By selectively extracting part of the incoming signals through the tunable wavelength and mode dependent drop filter, the in-line and switchable monitor can discriminate the wavelength, mode and power information of the transmitted signals. Being different from a conventional mode and wavelength demultiplexer, the monitor is specifically designed to ensure a flexible in-line monitoring. For demonstration, three mode and three wavelength multiplexed signals are successfully processed. Assisted by the integrated photodetectors (PDs), both the measured photo currents and eye diagrams validate the performance of the proposed device. The bit error ratio (BER) measurement results show less than 0.4 dB power penalty between different modes and ~2 dB power penalty for single wavelength and WDM cases under 10-9 BER level.

  14. Integrated 3D macro-trapping and light-sheet imaging system

    NASA Astrophysics Data System (ADS)

    Yang, Zhengyi; Piksarv, Peeter; Ferrier, David E. K.; Gunn-Moore, Frank J.; Dholakia, Kishan

    2015-08-01

    Biological research requires high-speed and low-damage imaging techniques for live specimens in areas such as development study in embryos. Light sheet microscopy provides fast imaging speed whilst keeps the photo-damage and photo-blenching to minimum. Conventional sample embedding methods in light sheet imaging involves using agent such as agarose which potentially affects the behavior and the develop pattern of the specimens. Here we demonstrate integrating dual-beam trapping method into light sheet imaging system to confine and translate the specimen whilst light sheet images are taken. Tobacco plant cells as well as Spirobranchus lamarcki larva were trapped solely with optical force and sectional images were acquired. This now approach has the potential to extend the applications of light sheet imaging significantly.

  15. Novel flexible Parylene neural probe with 3D sheath structure for enhancing tissue integration.

    PubMed

    Kuo, Jonathan T W; Kim, Brian J; Hara, Seth A; Lee, Curtis D; Gutierrez, Christian A; Hoang, Tuan Q; Meng, Ellis

    2013-02-21

    A Parylene C neural probe with a three dimensional sheath structure was designed, fabricated, and characterized. Multiple platinum (Pt) electrodes for recording neural signals were fabricated on both inner and outer surfaces of the sheath structure. Thermoforming of Parylene was used to create the three dimensional sheath structures from flat surface micromachined microchannels using solid microwires as molds. Benchtop electrochemical characterization was performed on the thin film Pt electrodes using cyclic voltammetry and electrochemical impedance spectroscopy and showed that electrodes possessed low impedances suitable for neuronal recordings. A procedure for implantation of the neural probe was developed and successfully demonstrated in vitro into an agarose brain tissue model. The electrode-lined sheath will be decorated with eluting neurotrophic factors to promote in vivo neural tissue ingrowth post-implantation. These features will enhance tissue integration and improve recording quality towards realizing reliable chronic neural interfaces.

  16. Writing of 3D optical integrated circuits with ultrashort laser pulses in the presence of strong spherical aberration

    NASA Astrophysics Data System (ADS)

    Bukharin, M. A.; Skryabin, N. N.; Khudyakov, D. V.; Vartapetov, S. K.

    2016-09-01

    A novel technique was proposed for 3D femtosecond writing of waveguides and optical integrated circuits in the presence of strong spherical aberration, caused by inscription at significantly different depth under the surface of optical glasses and crystals. Strong negative effect of spherical aberration and related asymmetry of created structures was reduced due to transition to the cumulative thermal regime of femtosecond interaction with the material. The differences in the influence of spherical aberration effect in a broad depth range (larger than 200 µm) was compensated by dynamic adjustment of laser pulse energy during the process of waveguides recording. The presented approach has been experimentally implemented in fused silica. Obtained results can be used in production of a broad class of femtosecond written three-dimensional integrated optical systems, inscripted at non-optimal (for focusing lens) optical depth or in significantly extended range of depths.

  17. 3D Rheological Modeling of NW Intraplate Europe, Deciphering Spatial Integrated strength patterns, Mechanical Strong Layering and EET

    NASA Astrophysics Data System (ADS)

    Beekman, F.; Hardebol, N.; Cloetingh, S.; Tesauro, M.

    2006-12-01

    Better understanding of 3D rheological heterogeneity of the European Lithosphere provide the key to tie the recorded intraplate deformation pattern to stress fields transmitted into plate interior from plate boundary forces. The first order strain patterns result from stresses transmitted through the European lithosphere that is marked by a patchwork of high strength variability from inherited structural and compositional heterogeneities and upper mantle thermal perturbations. As the lithospheric rheology depends primarily on its spatial structure, composition and thermal estate, the 3D strength model for the European lithosphere relies on a 3D compositional model that yields the compositional heterogeneities and an iteratively calculated thermal cube using Fouriers law for heat conduction. The accurate appraisal of spatial strength variability results from proper mapping and integration of the geophysical compositional and thermal input parameters. Therefore, much attention has been paid to a proper description of first order structural and tectonic features that facilitate compilation of the compositional and thermal input models. As such, the 3D strength model reflects the thermo-mechanical structure inherited from the Europeans polyphase deformation history. Major 3D spatial mechanical strength variability has been revealed. The East-European and Fennoscandian Craton to the NE exhibit high strength (30-50 1012 N/m) from low mantle temperatures and surface heatflow of 35-60 mW/m2 while central and western Europe reflect a polyphase Phanerozoic thermo- tectonic history. Here, regions with high rigidity are formed primarily by patches of thermally stabilized Variscan Massifs (e.g. Rhenish, Armorican, Bohemian, and Iberian Massif) with low heatflow and lithospheric thickness values (50-65 mW/m2; 110-150 km) yielding strengths of ~15-25 1012 N/m. In contrast, major axis of weakened lithosphere coincides with Cenozoic Rift System (e.g. Upper and Lower Rhine Grabens

  18. Integrated Semiconductor-based Diagnostics System for Multiplexed Genomic Amplification and Electrochemical Detection of Biothreat Agents

    DTIC Science & Technology

    2009-01-01

    contract effort focused on the development of an automated, cartridge-based genotyping system that integrates a multiplex polymerase chain reaction...REPORT b.ABSTRACT c. THIS PAGE ABSTRACT OF PAGES Kristian Roth $X u U U SAR 19b. TELEPHONE NUMBER 425-493-2368 Standard Form 298 (Rev 8/98...Biothreat Agents Report Title ABSTRACT This contract effort focused on the development of an automated, cartridge-based genotyping system that integrates a

  19. Functional morphology and integration of corvid skulls – a 3D geometric morphometric approach

    PubMed Central

    Kulemeyer, Christoph; Asbahr, Kolja; Gunz, Philipp; Frahnert, Sylke; Bairlein, Franz

    2009-01-01

    Background Sympatric corvid species have evolved differences in nesting, habitat choice, diet and foraging. Differences in the frequency with which corvid species use their repertoire of feeding techniques is expected to covary with bill-shape and with the frontal binocular field. Species that frequently probe are expected to have a relatively longer bill and more sidewise oriented orbits in contrast to species that frequently peck. We tested this prediction by analyzing computed tomography scans of skulls of six corvid species by means of three-dimensional geometric morphometrics. We (1) explored patterns of major variation using principal component analysis, (2) compared within and between species relationships of size and shape and (3) quantitatively compared patterns of morphological integration between bill and cranium by means of partial least squares (singular warp) analysis. Results Major shape variation occurs at the bill, in the orientation of orbits, in the position of the foramen magnum and in the angle between bill and cranium. The first principal component correlated positively with centroid-size, but within-species allometric relationships differed markedly. Major covariation between the bill and cranium lies in the difference in orbit orientation relative to bill-length and in the angle between bill and cranium. Conclusion Corvid species show pronounced differences in skull shape, which covary with foraging mode. Increasing bill-length, bill-curvature and sidewise orientation of the eyes is associated with an increase in the observed frequency in probing (vice versa in pecking). Hence, the frequency of probing, bill-length, bill-curvature and sidewise orientation of the eyes is progressively increased from jackdaw, to Eurasian jay, to black-billed magpie, to hooded crow, to rook and to common raven (when feeding on carcasses is considered as probing). Our results on the morphological integration suggest that most of the covariation between bill and

  20. RELAP5-3D Transient Modelling for NGNP Integrated Plant

    SciTech Connect

    Sabharwall, P.; Anderson, N. A.

    2014-06-01

    The High-Temperature Gas-cooled Reactor (HTGR) is designed with outlet temperatures ranging between 750°C and 800°C. These high outlet temperatures enhance the power production efficiency and facilitate a variety of industrial applications. The objective of this study is to understand the response of the primary system to potential transients in the secondary system. For this analysis, the transient condition originates in the Intermediate Heat Exchanger (IHX) or Steam Generator (SG) of the HTGR-integrated plant. The transients analysed are: a loss of pressure; loss of feedwater flow; inadvertent closure of main steam valve; decrease in returning gas temperature and heat load step change. The results show a large dependence on the negative reactivity added to the fuel as a function of increased temperature. The returning gas temperature decrease transient resulted in the highest fuel temperature (1361°C). Fuel temperature was shown to be less than the 1600°C fuel limit for each case analysed.

  1. IRIS Earthquake Browser with Integration to the GEON IDV for 3-D Visualization of Hypocenters.

    NASA Astrophysics Data System (ADS)

    Weertman, B. R.

    2007-12-01

    We present a new generation of web based earthquake query tool - the IRIS Earthquake Browser (IEB). The IEB combines the DMC's large set of earthquake catalogs (provided by USGS/NEIC, ISC and the ANF) with the popular Google Maps web interface. With the IEB you can quickly and easily find earthquakes in any region of the globe. Using Google's detailed satellite images, earthquakes can be easily co-located with natural geographic features such as volcanoes as well as man made features such as commercial mines. A set of controls allow earthquakes to be filtered by time, magnitude, and depth range as well as catalog name, contributor name and magnitude type. Displayed events can be easily exported in NetCDF format into the GEON Integrated Data Viewer (IDV) where hypocenters may be visualized in three dimensions. Looking "under the hood", the IEB is based on AJAX technology and utilizes REST style web services hosted at the IRIS DMC. The IEB is part of a broader effort at the DMC aimed at making our data holdings available via web services. The IEB is useful both educationally and as a research tool.

  2. Numerical Modeling of 3-D Dynamics of Ultrasound Contrast Agent Microbubbles Using the Boundary Integral Method

    NASA Astrophysics Data System (ADS)

    Calvisi, Michael; Manmi, Kawa; Wang, Qianxi

    2014-11-01

    Ultrasound contrast agents (UCAs) are microbubbles stabilized with a shell typically of lipid, polymer, or protein and are emerging as a unique tool for noninvasive therapies ranging from gene delivery to tumor ablation. The nonspherical dynamics of contrast agents are thought to play an important role in both diagnostic and therapeutic applications, for example, causing the emission of subharmonic frequency components and enhancing the uptake of therapeutic agents across cell membranes and tissue interfaces. A three-dimensional model for nonspherical contrast agent dynamics based on the boundary integral method is presented. The effects of the encapsulating shell are approximated by adapting Hoff's model for thin-shell, spherical contrast agents to the nonspherical case. A high-quality mesh of the bubble surface is maintained by implementing a hybrid approach of the Lagrangian method and elastic mesh technique. Numerical analyses for the dynamics of UCAs in an infinite liquid and near a rigid wall are performed in parameter regimes of clinical relevance. The results show that the presence of a coating significantly reduces the oscillation amplitude and period, increases the ultrasound pressure amplitude required to incite jetting, and reduces the jet width and velocity.

  3. Effect of Weaving Direction of Conductive Yarns on Electromagnetic Performance of 3D Integrated Microstrip Antenna

    NASA Astrophysics Data System (ADS)

    Xu, Fujun; Yao, Lan; Zhao, Da; Jiang, Muwen; Qiu, Yipping

    2013-10-01

    A three-dimensionally integrated microstrip antenna (3DIMA) is a microstrip antenna woven into the three-dimensional woven composite for load bearing while functioning as an antenna. In this study, the effect of weaving direction of conductive yarns on electromagnetic performance of 3DIMAs are investigated by designing, simulating and experimental testing of two microstrip antennas with different weaving directions of conductive yarns: one has the conductive yarns along the antenna feeding direction (3DIMA-Exp1) and the other has the conductive yarns perpendicular the antenna feeding direction (3DIMA-Exp2). The measured voltage standing wave ratio (VSWR) of 3DIMA-Exp1 was 1.4 at the resonant frequencies of 1.39 GHz; while that of 3DIMA-Exp2 was 1.2 at the resonant frequencies of 1.35 GHz. In addition, the measured radiation pattern of the 3DIMA-Exp1 has smaller back lobe and higher gain value than those of the 3DIMA-Exp2. This result indicates that the waving direction of conductive yarns may have a significant impact on electromagnetic performance of textile structural antennas.

  4. How integrating 3D LiDAR data in the dike surveillance protocol: The French case

    NASA Astrophysics Data System (ADS)

    Bretar, F.; Mériaux, P.; Fauchard, C.

    2012-04-01

    carried out. A LiDAR system is able to acquire data on a dike structure of up to 80 km per day, which makes the use of this technique also valuable in case of emergency situations. It provides additional valuable products like precious information on dike slopes and crest or their near environment (river banks, etc.). Moreover, in case of vegetation, LiDAR data makes possible to study hidden structures or defaults from images like the erosion of riverbanks under forestry vegetation. The possibility of studying the vegetation is also of high importance: the development of woody vegetation near or onto the dike is a major risk factor. Surface singularities are often signs of disorder or suspected disorder in the dike itself: for example a subsidence or a sinkhole on a ridge may result from internal erosion collapse. Finally, high resolution topographic data contribute to build specific geomechanical model of the dike that, after incorporating data provided by geophysical and geotechnical surveys, are integrated in the calculations of the structure stability. Integrating the regular use of LiDAR data in the dike surveillance protocol is not yet operational in France. However, the high number of French stakeholders at the national level (on average, there is one stakeholder for only 8-9km of dike !) and the real added value of LiDAR data makes a spatial data infrastructure valuable (webservices for processing the data, consulting and filling the database on the field when performing the local diagnosis)

  5. Monolithically integrated 20-channel optical add/drop multiplexer subsystem with hybrid-integrated 40-channel photodetector array

    NASA Astrophysics Data System (ADS)

    Schumacher, Andreas B.; Krabe, Detlef; Dieckroeger, Jens; Spott, Thorsten; Kraeker, Tobias; Martins, Evely; Zavrsnik, Miha; Schneider, Hartmut W.; Baumann, Ingo

    2003-03-01

    We built a 20 channel, 200 GHz, fully reconfigurable optical add-/drop multiplexer with integrated variable optical attenuators and power monitor diodes. A single planar lightwave circuit chip contains demultiplexer, switch array, attenuators and multiplexers. It also serves as an "optical motherboard" for a hybrid, flip-chip assembly containing four 10-channel photo detector arrays. A thermal management concept which considers both microscopic and macroscopic aspects of the device was developed. The final device exhibits an insertion loss of 9 dB from "in"- to "through"-port, a 1 dB bandwidth of >50 GHz and switch extinction ratios in excess of 40 dB.

  6. Progress on a New Integrated 3-D UCG Simulator and its Initial Application

    SciTech Connect

    Nitao, J J; Camp, D W; Buscheck, T A; White, J A; Burton, G C; Wagoner, J L; Chen, M

    2011-09-22

    A comprehensive simulator is being developed for underground coal gasification (UCG), with the capability to support site selection, design, hazard analyses, operations, and monitoring (Nitao et al., 2010). UCG is computationally challenging because it involves tightly-coupled multi-physical/chemical processes, with vastly different timescales. This new capability will predict cavity growth, product gas composition and rate, and the interaction with the host environment, accounting for site characteristics, injection gas composition and rate, and associated water-well extraction rates. Progress on the new simulator includes completion and system integration of a wall model, a rock spalling model, a cavity boundary tracking model, a one-dimensional cavity gas reactive transport model, a rudimentary rubble heat, mass, and reaction model, and coupling with a pre-existing hydrology simulator. An existing geomechanical simulator was enhanced to model cavity collapse and overburden subsidence. A commercial computational fluid dynamics (CFD) code is being evaluated to model cavity gas flow and combustion in two and three dimensions. Although the simulator is midway in its development, it was applied to modeling the Hoe Creek III field test (Stephens, 1981) conducted in the 1970s, in order to evaluate and demonstrate the simulator's basic capabilities, gain experience, and guide future development. Furthermore, it is consistent with our philosophy of incremental, spiral software development, which helps in identifying and resolving potential problems early in the process. The simulation accounts for two coal seams, two injection points, and air and oxygen phases. Approximate extent and shape of cavity growth showed reasonable agreement with interpreted field data. Product gas composition and carbon consumed could not be simultaneously matched for a given set of parameter values due to the rudimentary rubble model currently used, although they can be matched using separate

  7. Amplitude-modulated laser range-finder for 3D imaging with multi-sensor data integration capabilities

    NASA Astrophysics Data System (ADS)

    Bartolini, L.; Ferri de Collibus, M.; Fornetti, G.; Guarneri, M.; Paglia, E.; Poggi, C.; Ricci, R.

    2005-06-01

    A high performance Amplitude Modulated Laser Rangefinder (AM-LR) is presented, aimed at accurately reconstructing 3D digital models of real targets, either single objects or complex scenes. The scanning system enables to sweep the sounding beam either linearly across the object or circularly around it, by placing the object on a controlled rotating platform. Both phase shift and amplitude of the modulating wave of back-scattered light are collected and processed, resulting respectively in an accurate range image and a shade-free, high resolution, photographic-like intensity image. The best performances obtained in terms of range resolution are ~100 μm. Resolution itself can be made to depend mainly on the laser modulation frequency, provided that the power of the backscattered light reaching the detector is at least a few nW. 3D models are reconstructed from sampled points by using specifically developed software tools, optimized so as to take advantage of the system peculiarities. Special procedures have also been implemented to perform precise matching of data acquired independently with different sensors (LIF laser sensors, thermographic cameras, etc.) onto the 3D models generated using the AM-LR. The system has been used to scan different types of real surfaces (stone, wood, alloys, bones) and ca be applied in various fields, ranging from industrial machining to medical diagnostics, vision in hostile environments cultural heritage conservation and restoration. The relevance of this technology in cultural heritage applications is discussed in special detail, by providing results obtained in different campaigns with an emphasis on the system's multi-sensor data integration capabilities.

  8. Evaluation of a combined pre-processing and H.264-compression scheme for 3D integral images

    NASA Astrophysics Data System (ADS)

    Olsson, Roger; Sjöström, Mårten; Xu, Youzhi

    2007-01-01

    To provide sufficient 3D-depth fidelity, integral imaging (II) requires an increase in spatial resolution of several orders of magnitude from today's 2D images. We have recently proposed a pre-processing and compression scheme for still II-frames based on forming a pseudo video sequence (PVS) from sub images (SI), which is later coded using the H.264/MPEG-4 AVC video coding standard. The scheme has shown good performance on a set of reference images. In this paper we first investigate and present how five different ways to select the SIs when forming the PVS affect the schemes compression efficiency. We also study how the II-frame structure relates to the performance of a PVS coding scheme. Finally we examine the nature of the coding artifacts which are specific to the evaluated PVS-schemes. We can conclude that for all except the most complex reference image, all evaluated SI selection orders significantly outperforms JPEG 2000 where compression ratios of up to 342:1, while still keeping PSNR > 30 dB, is achieved. We can also confirm that when selecting PVS-scheme, the scheme which results in a higher PVS-picture resolution should be preferred to maximize compression efficiency. Our study of the coded II-frames also indicates that the SI-based PVS, contrary to other PVS schemes, tends to distribute its coding artifacts more homogenously over all 3D-scene depths.

  9. Integrating Dynamic Data and Sensors with Semantic 3D City Models in the Context of Smart Cities

    NASA Astrophysics Data System (ADS)

    Chaturvedi, K.; Kolbe, T. H.

    2016-10-01

    Smart cities provide effective integration of human, physical and digital systems operating in the built environment. The advancements in city and landscape models, sensor web technologies, and simulation methods play a significant role in city analyses and improving quality of life of citizens and governance of cities. Semantic 3D city models can provide substantial benefits and can become a central information backbone for smart city infrastructures. However, current generation semantic 3D city models are static in nature and do not support dynamic properties and sensor observations. In this paper, we propose a new concept called Dynamizer allowing to represent highly dynamic data and providing a method for injecting dynamic variations of city object properties into the static representation. The approach also provides direct capability to model complex patterns based on statistics and general rules and also, real-time sensor observations. The concept is implemented as an Application Domain Extension for the CityGML standard. However, it could also be applied to other GML-based application schemas including the European INSPIRE data themes and national standards for topography and cadasters like the British Ordnance Survey Mastermap or the German cadaster standard ALKIS.

  10. Towards a high performance vertex detector based on 3D integration of deep N-well MAPS

    NASA Astrophysics Data System (ADS)

    Re, V.

    2010-06-01

    The development of deep N-Well (DNW) CMOS active pixel sensors was driven by the ambitious goal of designing a monolithic device with similar functionalities as in hybrid pixel readout chips, such as pixel-level sparsification and time stamping. The implementation of the DNW MAPS concept in a 3D vertical integration process naturally leads the designer towards putting more intelligence in the chip and in the pixels themselves, achieving novel device structures based on the interconnection of two or more layers fabricated in the same technology. These devices are read out with a data-push scheme that makes it possible to use pixel data for the generation of a flexible level 1 track trigger, based on associative memories, with short latency and high efficiency. This paper gives an update of the present status of DNW MAPS design in both 2D and 3D versions, and presents a discussion of the architectures that are being devised for the Layer 0 of the SuperB Silicon Vertex Tracker.

  11. Investigation Of Integrating Three-Dimensional (3-D) Geometry Into The Visual Anatomical Injury Descriptor (Visual AID) Using WebGL

    DTIC Science & Technology

    2011-08-01

    into the Visual Anatomical Injury Descriptor (Visual AID) Using WebGL AUTUMN KULAGA MENTOR: PATRICK GILLICH WARFIGHTER SURVIVABILITY BRANCH...Investigation Of Integrating Three-Dimensional (3-D) Geometry Into The Visual Anatomical Injury Descriptor (Visual AID) Using WebGL 5a. CONTRACT...discusses the Web-based 3-D environment prototype being developed to understand the feasibility of integrating WebGL into Visual AID. Using WebGL will

  12. Compact spatial multiplexers for mode division multiplexing.

    PubMed

    Chen, Haoshuo; van Uden, Roy; Okonkwo, Chigo; Koonen, Ton

    2014-12-29

    Spatial multiplexer (SMUX) for mode division multiplexing (MDM) has evolved from mode-selective excitation, multiple-spot and photonic-lantern based solutions in order to minimize both mode-dependent loss (MDL) and coupler insertion loss (CIL). This paper discusses the implementation of all the three solutions by compact components in a small footprint. Moreover, the compact SMUX can be manufactured in mass production and packaged to assure high reliability. First, push-pull scheme and center launch based SMUXes are demonstrated on two mostly-popular photonic integration platforms: Silicon-on-insulator (SOI) and Indium Phosphide (InP) for selectively exciting LP01 and LP11 modes. 2-dimensional (2D) top-coupling by using vertical emitters is explored to provide a coupling interface between a few-mode fiber (FMF) and the photonic integrated SMUX. SOI-based grating couplers and InP-based 45° vertical mirrors are proposed and researched as vertical emitters in each platform. Second, a 3-spot SMUX is realized on an InP-based circuit through employing 45° vertical mirrors. Third, as a newly-emerging photonic integration platform, laser-inscribed 3D waveguide (3DW) technology is applied for a fully-packaged dual-channel 6-mode SMUX including two 6-core photonic lantern structures as mode multiplexer and demultiplexer, respectively.

  13. An integrated passive micromixer-magnetic separation-capillary electrophoresis microdevice for rapid and multiplex pathogen detection at the single-cell level.

    PubMed

    Jung, Jae Hwan; Kim, Gha-Young; Seo, Tae Seok

    2011-10-21

    Here we report an integrated microdevice consisting of an efficient passive mixer, a magnetic separation chamber, and a capillary electrophoretic microchannel in which DNA barcode assay, target pathogen separation, and barcode DNA capillary electrophoretic analysis were performed sequentially within 30 min for multiplex pathogen detection at the single-cell level. The intestine-shaped serpentine 3D micromixer provides a high mixing rate to generate magnetic particle-pathogenic bacteria-DNA barcode labelled AuNP complexes quantitatively. After magnetic separation and purification of those complexes, the barcode DNA strands were released and analyzed by the microfluidic capillary electrophoresis within 5 min. The size of the barcode DNA strand was controlled depending on the target bacteria (Staphylococcus aureus, Escherichia coli O157:H7, and Salmonella typhimurium), and the different elution time of the barcode DNA peak in the electropherogram allows us to recognize the target pathogen with ease in the monoplex as well as in the multiplex analysis. In addition, the quantity of the DNA barcode strand (∼10(4)) per AuNP is enough to be observed in the laser-induced confocal fluorescence detector, thereby making single-cell analysis possible. This novel integrated microdevice enables us to perform rapid, sensitive, and multiplex pathogen detection with sample-in-answer-out capability to be applied for biosafety testing, environmental screening, and clinical trials.

  14. Acquiring multi-viewpoint image of 3D object for integral imaging using synthetic aperture phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Jeong, Min-Ok; Kim, Nam; Park, Jae-Hyeung; Jeon, Seok-Hee; Gil, Sang-Keun

    2009-02-01

    We propose a method generating elemental images for the auto-stereoscopic three-dimensional display technique, integral imaging, using phase-shifting digital holography. Phase shifting digital holography is a way recording the digital hologram by changing phase of the reference beam and extracting the complex field of the object beam. Since all 3D information is captured by the phase-shifting digital holography, the elemental images for any specifications of the lens array can be generated from single phase-shifting digital holography. We expanded the viewing angle of the generated elemental image by using the synthetic aperture phase-shifting digital hologram. The principle of the proposed method is verified experimentally.

  15. Evaluating integration of inland bathymetry in the U.S. Geological Survey 3D Elevation Program, 2014

    USGS Publications Warehouse

    Miller-Corbett, Cynthia

    2016-09-01

    Inland bathymetry survey collections, survey data types, features, sources, availability, and the effort required to integrate inland bathymetric data into the U.S. Geological Survey 3D Elevation Program are assessed to help determine the feasibility of integrating three-dimensional water feature elevation data into The National Map. Available data from wading, acoustic, light detection and ranging, and combined technique surveys are provided by the U.S. Geological Survey, National Oceanic and Atmospheric Administration, U.S. Army Corps of Engineers, and other sources. Inland bathymetric data accessed through Web-hosted resources or contacts provide useful baseline parameters for evaluating survey types and techniques used for collection and processing, and serve as a basis for comparing survey methods and the quality of results. Historically, boat-mounted acoustic surveys have provided most inland bathymetry data. Light detection and ranging techniques that are beneficial in areas hard to reach by boat, that can collect dense data in shallow water to provide comprehensive coverage, and that can be cost effective for surveying large areas with good water clarity are becoming more common; however, optimal conditions and techniques for collecting and processing light detection and ranging inland bathymetry surveys are not yet well defined.Assessment of site condition parameters important for understanding inland bathymetry survey issues and results, and an evaluation of existing inland bathymetry survey coverage are proposed as steps to develop criteria for implementing a useful and successful inland bathymetry survey plan in the 3D Elevation Program. These survey parameters would also serve as input for an inland bathymetry survey data baseline. Integration and interpolation techniques are important factors to consider in developing a robust plan; however, available survey data are usually in a triangulated irregular network format or other format compatible with

  16. Integrated monolithic 3D MEMS scanner for switchable real time vertical/horizontal cross-sectional imaging.

    PubMed

    Li, Haijun; Duan, Xiyu; Qiu, Zhen; Zhou, Quan; Kurabayashi, Katsuo; Oldham, Kenn R; Wang, Thomas D

    2016-02-08

    We present an integrated monolithic, electrostatic 3D MEMS scanner with a compact chip size of 3.2 × 2.9 mm(2). Use of parametric excitation near resonance frequencies produced large optical deflection angles up to ± 27° and ± 28.5° in the X- and Y-axes and displacements up to 510 μm in the Z-axis with low drive voltages at atmospheric pressure. When packaged in a dual axes confocal endomicroscope, horizontal and vertical cross-sectional images can be collected seamlessly in tissue with a large field-of-view of >1 × 1 mm(2) and 1 × 0.41 mm(2), respectively, at 5 frames/sec.

  17. Extended depth-of-focus 3D micro integral imaging display using a bifocal liquid crystal lens.

    PubMed

    Shen, Xin; Wang, Yu-Jen; Chen, Hung-Shan; Xiao, Xiao; Lin, Yi-Hsin; Javidi, Bahram

    2015-02-15

    We present a three dimensional (3D) micro integral imaging display system with extended depth of focus by using a polarized bifocal liquid crystal lens. This lens and other optical components are combined as the relay optical element. The focal length of the relay optical element can be controlled to project an elemental image array in multiple positions with various lenslet image planes, by applying different voltages to the liquid crystal lens. The depth of focus of the proposed system can therefore be extended. The feasibility of our proposed system is experimentally demonstrated. In our experiments, the depth of focus of the display system is extended from 3.82 to 109.43 mm.

  18. GPU-based, parallel-line, omni-directional integration of measured acceleration field to obtain the 3D pressure distribution

    NASA Astrophysics Data System (ADS)

    Wang, Jin; Zhang, Cao; Katz, Joseph

    2016-11-01

    A PIV based method to reconstruct the volumetric pressure field by direct integration of the 3D material acceleration directions has been developed. Extending the 2D virtual-boundary omni-directional method (Omni2D, Liu & Katz, 2013), the new 3D parallel-line omni-directional method (Omni3D) integrates the material acceleration along parallel lines aligned in multiple directions. Their angles are set by a spherical virtual grid. The integration is parallelized on a Tesla K40c GPU, which reduced the computing time from three hours to one minute for a single realization. To validate its performance, this method is utilized to calculate the 3D pressure fields in isotropic turbulence and channel flow using the JHU DNS Databases (http://turbulence.pha.jhu.edu). Both integration of the DNS acceleration as well as acceleration from synthetic 3D particles are tested. Results are compared to other method, e.g. solution to the Pressure Poisson Equation (e.g. PPE, Ghaemi et al., 2012) with Bernoulli based Dirichlet boundary conditions, and the Omni2D method. The error in Omni3D prediction is uniformly low, and its sensitivity to acceleration errors is local. It agrees with the PPE/Bernoulli prediction away from the Dirichlet boundary. The Omni3D method is also applied to experimental data obtained using tomographic PIV, and results are correlated with deformation of a compliant wall. ONR.

  19. A probabilistic approach to jointly integrate 3D/4D seismic, production data and geological information for building reservoir models

    NASA Astrophysics Data System (ADS)

    Castro, Scarlet A.

    Reservoir modeling aims at understanding static and dynamic components of the reservoir in order to make decisions about future surface operations. The practice of reservoir modeling calls for the integration of expertise from different disciplines, as well as the in tegration of a wide variety of data: geological data, (core data, well-logs, etc.), production data (fluid rates or volumes, pressure data, etc.), and geophysical data (3D seismic data). Although a single 3D seismic survey is the most common geophysical data available for most reservoirs, a suite of several 3D seismic surveys (4D seismic data) acquired for monitoring production can be available for mature reservoirs. The main contribution of this dissertation is to incorporate 4D seismic data within the reservoir modeling workflow while honoring all other available data. This dissertation proposes two general approaches to include 4D seismic data into the reservoir modeling workflow. The Probabilistic Data Integration approach (PDI), which consists of modeling the information content of 4D seismic through a spatial probability of facies occurrence; and the Forward Modeling (FM) approach, which consists of matching 4D seismic along with production data. The FM approach requires forward modeling the 4D seismic response, which requires to downscale the flow simulation response. This dissertation introduces a novel dynamic downscaling method that takes into account both static information (high-resolution per meability field) and dynamic information in the form of coarsened fluxes and saturations (flow simulation on the coarsened grid). The two proposed approaches (PDI and FM approaches) are applied to a prominent field in the North Sea, to model the channel facies of a fluvial reservoir. The PDI approach constrained the reservoir model to the spatial probability of facies occurrence (obtained from a calibration between well-log and 4D seismic data) as well as other static data while satisfactorily history

  20. Integration (Phase 3D),

    DTIC Science & Technology

    2007-11-02

    tension rods will be drilled prior to assembly. fixing AFA Point 10 gap 30 grillage 35 Detail at floor floor User cabinet ANS&A to consider providing...duplicate mount for AFA connector panels. ANS&A would remove AFA panel and mount in user cabinet. Termination panels AFA propose mounting points on...clearance holes in the boom divider for AFA . boom divider At the centre of the arm, the cable trays terminate against the drive box. Three cable support rails

  1. 3-D imaging using row-column-addressed arrays with integrated apodization - part i: apodization design and line element beamforming.

    PubMed

    Rasmussen, Morten Fischer; Christiansen, Thomas Lehrmann; Thomsen, Erik Vilain; Jensen, Jørgen Arendt

    2015-05-01

    This paper investigates the effect of transducerintegrated apodization in row-column-addressed arrays and presents a beamforming approach specific for such arrays. Row-column addressing 2-D arrays greatly reduces the number of active channels needed to acquire a 3-D volume. A disadvantage of row-column-addressed arrays is an apparent ghost effect in the point spread function caused by edge waves. This paper investigates the origin of the edge waves and the effect of introducing an integrated apodization to reduce the ghost echoes. The performance of a λ/2-pitch 5-MHz 128 + 128 row-column-addressed array with different apodizations is simulated. A Hann apodization is shown to decrease imaging performance away from the center axis of the array because of a decrease in main lobe amplitude. Instead, a static roll-off apodization region located at the ends of the line elements is proposed. In simulations, the peak ghost echo intensity of a scatterer at (x,y, z) = (8, 3, 30) mm was decreased by 43 dB by integrating roll-off apodization into the array. The main lobe was unaffected by the apodization. Simulations of a 3-mm-diameter anechoic blood vessel at 30 mm depth showed that applying the transducer-integrated apodization increased the apparent diameter of the vessel from 2.0 mm to 2.4 mm, corresponding to an increase from 67% to 80% of the true vessel diameter. The line element beamforming approach is shown to be essential for achieving correct time-of-flight calculations, and hence avoid geometrical distortions. In Part II of this work, experimental results from a capacitive micromachined ultrasonic transducer with integrated roll-off apodization are given to validate the effect of integrating apodization into the line elements.

  2. Compilation of an integrated 3D soil and agrogeological database for the hydrophysical characterization of the unsaturated zone

    NASA Astrophysics Data System (ADS)

    Bakacsi, Zsófia; Kuti, László; Pásztor, László; Vatai, József; Szabó, József; Müller, Tamás.

    2010-05-01

    Describing the water movement in the unsaturated zone, numerous soil hydraulic data as input parameter are required concerning the water retention curve and the hydraulic conductivity function as the main hydraulic properties. The direct measurements of the hydraulic parameters are quite difficult and time-consuming; the estimation of them can be an alternative especially for large areas. The most commonly used basis of the estimation is the particle-size distribution (PSD) data or texture class. The aim of our work was to compile an integrated and harmonized 3D pedo- and agrogeological database with the physical properties and stratification of the formations to the depth of the permanent groundwater level, which describes the unsaturated zone in a 690 km2 pilot area. Since the existing pedo- and agrogeological databases are not able to serve separately these 3D model requirements, their integration was necessary. Due to its appropriate spatial and thematic resolution and data processing status, the Digital Kreybig Soil Information System (DKSIS) was chosen as pedological data source of the 3D model. The DKSIS has been compiled in the Research Institute for Soil Science and Agricultural Chemistry of the Hungarian Academy of Sciences, based on the 1:25,000 scale, national soil mapping program in Hungary. The survey sheets indicate the location of the observation sites. Due to the lack of measured PSD data, the field estimation of the textural classes, and the so-called "capillary rise of water" were used for the definition of the texture classes. The measured water uptake is supposed to have good relation with the textural class of the sample. During the data processing the inconsistent fields vs. capillary data pairs were excluded. In the DKSIS 649 polygons cover the pilot area, 484 soil profiles are occurring and characteristically each profile has two or three horizons. The agrogeological dataset is maintained by the Hungarian Geological Institute and derives

  3. Rapid Reconstitution Packages (RRPs) implemented by integration of computational fluid dynamics (CFD) and 3D printed microfluidics.

    PubMed

    Chi, Albert; Curi, Sebastian; Clayton, Kevin; Luciano, David; Klauber, Kameron; Alexander-Katz, Alfredo; D'hers, Sebastian; Elman, Noel M

    2014-08-01

    Rapid Reconstitution Packages (RRPs) are portable platforms that integrate microfluidics for rapid reconstitution of lyophilized drugs. Rapid reconstitution of lyophilized drugs using standard vials and syringes is an error-prone process. RRPs were designed using computational fluid dynamics (CFD) techniques to optimize fluidic structures for rapid mixing and integrating physical properties of targeted drugs and diluents. Devices were manufactured using stereo lithography 3D printing for micrometer structural precision and rapid prototyping. Tissue plasminogen activator (tPA) was selected as the initial model drug to test the RRPs as it is unstable in solution. tPA is a thrombolytic drug, stored in lyophilized form, required in emergency settings for which rapid reconstitution is of critical importance. RRP performance and drug stability were evaluated by high-performance liquid chromatography (HPLC) to characterize release kinetics. In addition, enzyme-linked immunosorbent assays (ELISAs) were performed to test for drug activity after the RRPs were exposed to various controlled temperature conditions. Experimental results showed that RRPs provided effective reconstitution of tPA that strongly correlated with CFD results. Simulation and experimental results show that release kinetics can be adjusted by tuning the device structural dimensions and diluent drug physical parameters. The design of RRPs can be tailored for a number of applications by taking into account physical parameters of the active pharmaceutical ingredients (APIs), excipients, and diluents. RRPs are portable platforms that can be utilized for reconstitution of emergency drugs in time-critical therapies.

  4. Integration of 3D Printed and Micropatterned Polycaprolactone Scaffolds for Guidance of Oriented Collagenous Tissue Formation In vivo

    PubMed Central

    Pilipchuk, Sophia P; Monje, Alberto; Jiao, Yizu; Hao, Jie; Kruger, Laura; Flanagan, Colleen L; Hollister, Scott J

    2016-01-01

    Scaffold design incorporating multi-scale cues for clinically-relevant, aligned tissue regeneration has potential to improve structural and functional integrity of multi-tissue interfaces. The objective of this pre-clinical study was to develop poly(ε-caprolactone) (PCL) scaffolds with mesoscale and microscale architectural cues specific to human ligament progenitor cells and assess their ability to form aligned bone-ligament-cementum complexes in vivo. PCL scaffolds were designed to integrate a 3D printed bone region with a micropatterned PCL thin film consisting of grooved pillars. The patterned film region was seeded with human ligament cells, fibroblasts transduced with BMP-7 genes seeded within the bone region, and a tooth dentin segment positioned on the ligament region prior to subcutaneous implantation into a murine model. Results indicated increased tissue alignment in vivo using micropatterned PCL films, compared to random-porous PCL. At 6 weeks, 30um groove depth significantly enhanced oriented collagen fiber thickness, overall cell alignment, and nuclear elongation relative to 10um groove depth. This study demonstrates for the first time that scaffolds with combined hierarchical mesoscale and microscale features can align cells in vivo for oral tissue repair with potential for improving the regenerative response of other bone-ligament complexes. PMID:26820240

  5. An intelligent recovery progress evaluation system for ACL reconstructed subjects using integrated 3-D kinematics and EMG features.

    PubMed

    Malik, Owais A; Senanayake, S M N Arosha; Zaheer, Dansih

    2015-03-01

    An intelligent recovery evaluation system is presented for objective assessment and performance monitoring of anterior cruciate ligament reconstructed (ACL-R) subjects. The system acquires 3-D kinematics of tibiofemoral joint and electromyography (EMG) data from surrounding muscles during various ambulatory and balance testing activities through wireless body-mounted inertial and EMG sensors, respectively. An integrated feature set is generated based on different features extracted from data collected for each activity. The fuzzy clustering and adaptive neuro-fuzzy inference techniques are applied to these integrated feature sets in order to provide different recovery progress assessment indicators (e.g., current stage of recovery, percentage of recovery progress as compared to healthy group, etc.) for ACL-R subjects. The system was trained and tested on data collected from a group of healthy and ACL-R subjects. For recovery stage identification, the average testing accuracy of the system was found above 95% (95-99%) for ambulatory activities and above 80% (80-84%) for balance testing activities. The overall recovery evaluation performed by the proposed system was found consistent with the assessment made by the physiotherapists using standard subjective/objective scores. The validated system can potentially be used as a decision supporting tool by physiatrists, physiotherapists, and clinicians for quantitative rehabilitation analysis of ACL-R subjects in conjunction with the existing recovery monitoring systems.

  6. Terrestrial and Aerial Laser Scanning Data Integration Using Wavelet Analysis for the Purpose of 3D Building Modeling

    PubMed Central

    Kedzierski, Michal; Fryskowska, Anna

    2014-01-01

    Visualization techniques have been greatly developed in the past few years. Three-dimensional models based on satellite and aerial imagery are now being enhanced by models generated using Aerial Laser Scanning (ALS) data. The most modern of such scanning systems have the ability to acquire over 50 points per square meter and to register a multiple echo, which allows the reconstruction of the terrain together with the terrain cover. However, ALS data accuracy is less than 10 cm and the data is often incomplete: there is no information about ground level (in most scanning systems), and often around the facade or structures which have been covered by other structures. However, Terrestrial Laser Scanning (TLS) not only acquires higher accuracy data (1–5 cm) but is also capable of registering those elements which are incomplete or not visible using ALS methods (facades, complicated structures, interiors, etc.). Therefore, to generate a complete 3D model of a building in high Level of Details, integration of TLS and ALS data is necessary. This paper presents the wavelet-based method of processing and integrating data from ALS and TLS. Methods of choosing tie points to combine point clouds in different datum will be analyzed. PMID:25004157

  7. Terrestrial and aerial laser scanning data integration using wavelet analysis for the purpose of 3D building modeling.

    PubMed

    Kedzierski, Michal; Fryskowska, Anna

    2014-07-07

    Visualization techniques have been greatly developed in the past few years. Three-dimensional models based on satellite and aerial imagery are now being enhanced by models generated using Aerial Laser Scanning (ALS) data. The most modern of such scanning systems have the ability to acquire over 50 points per square meter and to register a multiple echo, which allows the reconstruction of the terrain together with the terrain cover. However, ALS data accuracy is less than 10 cm and the data is often incomplete: there is no information about ground level (in most scanning systems), and often around the facade or structures which have been covered by other structures. However, Terrestrial Laser Scanning (TLS) not only acquires higher accuracy data (1-5 cm) but is also capable of registering those elements which are incomplete or not visible using ALS methods (facades, complicated structures, interiors, etc.). Therefore, to generate a complete 3D model of a building in high Level of Details, integration of TLS and ALS data is necessary. This paper presents the wavelet-based method of processing and integrating data from ALS and TLS. Methods of choosing tie points to combine point clouds in different datum will be analyzed.

  8. Integration of 3D Printed and Micropatterned Polycaprolactone Scaffolds for Guidance of Oriented Collagenous Tissue Formation In Vivo.

    PubMed

    Pilipchuk, Sophia P; Monje, Alberto; Jiao, Yizu; Hao, Jie; Kruger, Laura; Flanagan, Colleen L; Hollister, Scott J; Giannobile, William V

    2016-03-01

    Scaffold design incorporating multiscale cues for clinically relevant, aligned tissue regeneration has potential to improve structural and functional integrity of multitissue interfaces. The objective of this preclinical study is to develop poly(ε-caprolactone) (PCL) scaffolds with mesoscale and microscale architectural cues specific to human ligament progenitor cells and assess their ability to form aligned bone-ligament-cementum complexes in vivo. PCL scaffolds are designed to integrate a 3D printed bone region with a micropatterned PCL thin film consisting of grooved pillars. The patterned film region is seeded with human ligament cells, fibroblasts transduced with bone morphogenetic protein-7 genes seeded within the bone region, and a tooth dentin segment positioned on the ligament region prior to subcutaneous implantation into a murine model. Results indicate increased tissue alignment in vivo using micropatterned PCL films, compared to random-porous PCL. At week 6, 30 μm groove depth significantly enhances oriented collagen fiber thickness, overall cell alignment, and nuclear elongation relative to 10 μm groove depth. This study demonstrates for the first time that scaffolds with combined hierarchical mesoscale and microscale features can align cells in vivo for oral tissue repair with potential for improving the regenerative response of other bone-ligament complexes.

  9. Characterization of the Effect of thermal Cycling on the Signal Integrity of Interconnect Structures used in 3D Integrated Circuits

    NASA Astrophysics Data System (ADS)

    Kandel, Binayak

    2012-03-01

    The performance and reliability of the microelectronic devices are significantly influenced by the condition of interconnects in Integrated Circuits (IC). These interconnects serve primarily as signal transmission pathways in IC. Good interconnects enable free flow of electrical signals with low impedance. However, microelectronic devices are continuously subjected to fluctuating temperature conditions during their lifetime, which affect the signal integrity of interconnects. Therefore, this project takes a look at the effect of repeated temperature cycling on the reliability and performance of interconnects. Two types of interconnects: Back-End-of-Line (BEOL) and Through-Si-Via (TSV) were studied. We simulate the real world conditions by applying repeated temperature cycling, and use an RF network analyzer to extract the reflection and transmission signal characteristics of the interconnects. The mean-time-to-failure is determined upon their breakdown which is followed by the failure analysis to determine the root cause of failure.

  10. A General Method for Evaluation of 2D and 3D Domain Integrals Without Domain Discretization and its Application in BEM

    NASA Astrophysics Data System (ADS)

    Hematiyan, M. R.

    2007-03-01

    A robust method is presented to evaluate 2D and 3D domain integrals without domain discretization. Each domain integral is transformed into a double integral, a boundary integral and a 1D integral. Both integrals are evaluated by adaptive Simpson quadrature method. The method can be used to evaluate domain integrals over simply or multiply connected regions with any arbitrary form of integrands. As an application of the method, domain integrals produced in boundary element formulation of potential and elastostatic problems are analyzed. Several examples are provided to show the validity and accuracy of the method.

  11. Extended depth-of-field 3D endoscopy with synthetic aperture integral imaging using an electrically tunable focal-length liquid-crystal lens.

    PubMed

    Wang, Yu-Jen; Shen, Xin; Lin, Yi-Hsin; Javidi, Bahram

    2015-08-01

    Conventional synthetic-aperture integral imaging uses a lens array to sense the three-dimensional (3D) object or scene that can then be reconstructed digitally or optically. However, integral imaging generally suffers from a fixed and limited range of depth of field (DOF). In this Letter, we experimentally demonstrate a 3D integral-imaging endoscopy with tunable DOF by using a single large-aperture focal-length-tunable liquid crystal (LC) lens. The proposed system can provide high spatial resolution and an extended DOF in synthetic-aperture integral imaging 3D endoscope. In our experiments, the image plane in the integral imaging pickup process can be tuned from 18 to 38 mm continuously using a large-aperture LC lens, and the total DOF is extended from 12 to 51 mm. To the best of our knowledge, this is the first report on synthetic aperture integral imaging 3D endoscopy with a large-aperture LC lens that can provide high spatial resolution 3D imaging with an extend DOF.

  12. Direct profiling of cancer biomarkers in tumor tissue using a multiplexed nanostructured microelectrode integrated circuit.

    PubMed

    Fang, Zhichao; Soleymani, Leyla; Pampalakis, Georgios; Yoshimoto, Maisa; Squire, Jeremy A; Sargent, Edward H; Kelley, Shana O

    2009-10-27

    The analysis of panels of nucleic acid biomarkers offers valuable diagnostic and prognostic information for cancer management. A cost-effective, highly sensitive electronic chip would offer an ideal platform for clinical biomarker readout and would have maximal utility if it was (i) multiplexed, enabling on-chip assays of multiple biomarkers, and (ii) able to perform direct (PCR-free) readout of disease-related genes. Here we report a chip onto which we integrate novel nanostructured microelectrodes and with which we directly detect cancer biomarkers in heterogeneous biological samples-both cell extracts and tumor tissues. Coarse photolithographic microfabrication defines a multiplexed sensing array; bottom-up fabrication of nanostructured microelectrodes then provides sensing elements. We analyzed a panel of mRNA samples for prostate cancer related gene fusions using the chip. We accurately identified gene fusions that correlate with aggressive prostate cancer and distinguished these from fusions associated with slower-progressing forms of the disease. The multiplexed nanostructured microelectrode integrated circuit reported herein provides direct, amplification-free, sample-to-answer in under 1 h using the 10 ng of mRNA readily available in biopsy samples.

  13. Critical Analysis and Digital Reconstructions of Alberti's Architectures by the Use of 3d Morphometric Integrated Survey Database

    NASA Astrophysics Data System (ADS)

    Ferrari, F.; Medici, M.

    2017-02-01

    Since 2005, DIAPReM Centre of the Department of Architecture of the University of Ferrara, in collaboration with the "Centro Studi Leon Battista Alberti" Foundation and the Consorzio Futuro in Ricerca, is carrying out a research project for the creation of 3D databases that could allow the development of a critical interpretation of Alberti's architectural work. The project is primarily based on a common three-dimensional integrated survey methodology for the creation of a navigable multilayered database. The research allows the possibility of reiterative metrical analysis, thanks to the use of a coherent data in order to check and validate hypothesis by researchers, art historians and scholars on Alberti's architectural work. Coherently with this methodological framework, indeed, two case studies are explained in this paper: the church of San Sebastiano in Matua and The Church of the Santissima Annunziata in Florence. Furthermore, thanks to a brief introduction of further developments of the project, a short graphical analysis of preliminary results on Tempio Malatestiano in Rimini opens new perspectives of research.

  14. ePlant and the 3D Data Display Initiative: Integrative Systems Biology on the World Wide Web

    PubMed Central

    Fucile, Geoffrey; Di Biase, David; Nahal, Hardeep; La, Garon; Khodabandeh, Shokoufeh; Chen, Yani; Easley, Kante; Christendat, Dinesh; Kelley, Lawrence; Provart, Nicholas J.

    2011-01-01

    Visualization tools for biological data are often limited in their ability to interactively integrate data at multiple scales. These computational tools are also typically limited by two-dimensional displays and programmatic implementations that require separate configurations for each of the user's computing devices and recompilation for functional expansion. Towards overcoming these limitations we have developed “ePlant” (http://bar.utoronto.ca/eplant) – a suite of open-source world wide web-based tools for the visualization of large-scale data sets from the model organism Arabidopsis thaliana. These tools display data spanning multiple biological scales on interactive three-dimensional models. Currently, ePlant consists of the following modules: a sequence conservation explorer that includes homology relationships and single nucleotide polymorphism data, a protein structure model explorer, a molecular interaction network explorer, a gene product subcellular localization explorer, and a gene expression pattern explorer. The ePlant's protein structure explorer module represents experimentally determined and theoretical structures covering >70% of the Arabidopsis proteome. The ePlant framework is accessed entirely through a web browser, and is therefore platform-independent. It can be applied to any model organism. To facilitate the development of three-dimensional displays of biological data on the world wide web we have established the “3D Data Display Initiative” (http://3ddi.org). PMID:21249219

  15. ePlant and the 3D data display initiative: integrative systems biology on the world wide web.

    PubMed

    Fucile, Geoffrey; Di Biase, David; Nahal, Hardeep; La, Garon; Khodabandeh, Shokoufeh; Chen, Yani; Easley, Kante; Christendat, Dinesh; Kelley, Lawrence; Provart, Nicholas J

    2011-01-10

    Visualization tools for biological data are often limited in their ability to interactively integrate data at multiple scales. These computational tools are also typically limited by two-dimensional displays and programmatic implementations that require separate configurations for each of the user's computing devices and recompilation for functional expansion. Towards overcoming these limitations we have developed "ePlant" (http://bar.utoronto.ca/eplant) - a suite of open-source world wide web-based tools for the visualization of large-scale data sets from the model organism Arabidopsis thaliana. These tools display data spanning multiple biological scales on interactive three-dimensional models. Currently, ePlant consists of the following modules: a sequence conservation explorer that includes homology relationships and single nucleotide polymorphism data, a protein structure model explorer, a molecular interaction network explorer, a gene product subcellular localization explorer, and a gene expression pattern explorer. The ePlant's protein structure explorer module represents experimentally determined and theoretical structures covering >70% of the Arabidopsis proteome. The ePlant framework is accessed entirely through a web browser, and is therefore platform-independent. It can be applied to any model organism. To facilitate the development of three-dimensional displays of biological data on the world wide web we have established the "3D Data Display Initiative" (http://3ddi.org).

  16. Direct laser-writing of ferroelectric single-crystal waveguide architectures in glass for 3D integrated optics

    PubMed Central

    Stone, Adam; Jain, Himanshu; Dierolf, Volkmar; Sakakura, Masaaki; Shimotsuma, Yasuhiko; Miura, Kiyotaka; Hirao, Kazuyuki; Lapointe, Jerome; Kashyap, Raman

    2015-01-01

    Direct three-dimensional laser writing of amorphous waveguides inside glass has been studied intensely as an attractive route for fabricating photonic integrated circuits. However, achieving essential nonlinear-optic functionality in such devices will also require the ability to create high-quality single-crystal waveguides. Femtosecond laser irradiation is capable of crystallizing glass in 3D, but producing optical-quality single-crystal structures suitable for waveguiding poses unique challenges that are unprecedented in the field of crystal growth. In this work, we use a high angular-resolution electron diffraction method to obtain the first conclusive confirmation that uniform single crystals can be grown inside glass by femtosecond laser writing under optimized conditions. We confirm waveguiding capability and present the first quantitative measurement of power transmission through a laser-written crystal-in-glass waveguide, yielding loss of 2.64 dB/cm at 1530 nm. We demonstrate uniformity of the crystal cross-section down the length of the waveguide and quantify its birefringence. Finally, as a proof-of-concept for patterning more complex device geometries, we demonstrate the use of dynamic phase modulation to grow symmetric crystal junctions with single-pass writing. PMID:25988599

  17. High-κ Al2O3 material in low temperature wafer-level bonding for 3D integration application

    NASA Astrophysics Data System (ADS)

    Fan, J.; Tu, L. C.; Tan, C. S.

    2014-03-01

    This work systematically investigated a high-κ Al2O3 material for low temperature wafer-level bonding for potential applications in 3D microsystems. A clean Si wafer with an Al2O3 layer thickness of 50 nm was applied as our experimental approach. Bonding was initiated in a clean room ambient after surface activation, followed by annealing under inert ambient conditions at 300 °C for 3 h. The investigation consisted of three parts: a mechanical support study using the four-point bending method, hermeticity measurements using the helium bomb test, and thermal conductivity analysis for potential heterogeneous bonding. Compared with samples bonded using a conventional oxide bonding material (SiO2), a higher interfacial adhesion energy (˜11.93 J/m2) and a lower helium leak rate (˜6.84 × 10-10 atm.cm3/sec) were detected for samples bonded using Al2O3. More importantly, due to the excellent thermal conductivity performance of Al2O3, this technology can be used in heterogeneous direct bonding, which has potential applications for enhancing the performance of Si photonic integrated devices.

  18. 3D Numerical Optimization Modelling of Ivancich landslides (Assisi, Italy) via integration of remote sensing and in situ observations.

    NASA Astrophysics Data System (ADS)

    Castaldo, Raffaele; De Novellis, Vincenzo; Lollino, Piernicola; Manunta, Michele; Tizzani, Pietro

    2015-04-01

    The new challenge that the research in slopes instabilities phenomena is going to tackle is the effective integration and joint exploitation of remote sensing measurements with in situ data and observations to study and understand the sub-surface interactions, the triggering causes, and, in general, the long term behaviour of the investigated landslide phenomenon. In this context, a very promising approach is represented by Finite Element (FE) techniques, which allow us to consider the intrinsic complexity of the mass movement phenomena and to effectively benefit from multi source observations and data. In this context, we perform a three dimensional (3D) numerical model of the Ivancich (Assisi, Central Italy) instability phenomenon. In particular, we apply an inverse FE method based on a Genetic Algorithm optimization procedure, benefitting from advanced DInSAR measurements, retrieved through the full resolution Small Baseline Subset (SBAS) technique, and an inclinometric array distribution. To this purpose we consider the SAR images acquired from descending orbit by the COSMO-SkyMed (CSK) X-band radar constellation, from December 2009 to February 2012. Moreover the optimization input dataset is completed by an array of eleven inclinometer measurements, from 1999 to 2006, distributed along the unstable mass. The landslide body is formed of debris material sliding on a arenaceous marl substratum, with a thin shear band detected using borehole and inclinometric data, at depth ranging from 20 to 60 m. Specifically, we consider the active role of this shear band in the control of the landslide evolution process. A large field monitoring dataset of the landslide process, including at-depth piezometric and geological borehole observations, were available. The integration of these datasets allows us to develop a 3D structural geological model of the considered slope. To investigate the dynamic evolution of a landslide, various physical approaches can be considered

  19. 3D documentation and visualization of external injury findings by integration of simple photography in CT/MRI data sets (IprojeCT).

    PubMed

    Campana, Lorenzo; Breitbeck, Robert; Bauer-Kreuz, Regula; Buck, Ursula

    2016-05-01

    This study evaluated the feasibility of documenting patterned injury using three dimensions and true colour photography without complex 3D surface documentation methods. This method is based on a generated 3D surface model using radiologic slice images (CT) while the colour information is derived from photographs taken with commercially available cameras. The external patterned injuries were documented in 16 cases using digital photography as well as highly precise photogrammetry-supported 3D structured light scanning. The internal findings of these deceased were recorded using CT and MRI. For registration of the internal with the external data, two different types of radiographic markers were used and compared. The 3D surface model generated from CT slice images was linked with the photographs, and thereby digital true-colour 3D models of the patterned injuries could be created (Image projection onto CT/IprojeCT). In addition, these external models were merged with the models of the somatic interior. We demonstrated that 3D documentation and visualization of external injury findings by integration of digital photography in CT/MRI data sets is suitable for the 3D documentation of individual patterned injuries to a body. Nevertheless, this documentation method is not a substitution for photogrammetry and surface scanning, especially when the entire bodily surface is to be recorded in three dimensions including all external findings, and when precise data is required for comparing highly detailed injury features with the injury-inflicting tool.

  20. Integral imaging microscopy with enhanced depth-of-field using a spatial multiplexing.

    PubMed

    Kwon, Ki-Chul; Erdenebat, Munkh-Uchral; Alam, Md Ashraful; Lim, Young-Tae; Kim, Kwang Gi; Kim, Nam

    2016-02-08

    A depth-of-field enhancement method for integral imaging microscopy system using a spatial multiplexing structure consisting of a beamsplitter with dual video channels and micro lens arrays is proposed. A computational integral imaging reconstruction algorithm generates two sets of depth-sliced images for the acquired depth information of the captured elemental image arrays and the well-focused depth-slices of both image sets are combined where each is focused on a different depth plane of the specimen. A prototype is implemented, and the experimental results demonstrate that the depth-of-field of the reconstructed images in the proposed integral imaging microscopy is significantly increased compared with conventional integral imaging microscopy systems.

  1. Controlling Shear Stress in 3D Bioprinting is a Key Factor to Balance Printing Resolution and Stem Cell Integrity.

    PubMed

    Blaeser, Andreas; Duarte Campos, Daniela Filipa; Puster, Uta; Richtering, Walter; Stevens, Molly M; Fischer, Horst

    2016-02-04

    A microvalve-based bioprinting system for the manufacturing of high-resolution, multimaterial 3D-structures is reported. Applying a straightforward fluid-dynamics model, the shear stress at the nozzle site can precisely be controlled. Using this system, a broad study on how cell viability and proliferation potential are affected by different levels of shear stress is conducted. Complex, multimaterial 3D structures are printed with high resolution. This work pioneers the investigation of shear stress-induced cell damage in 3D bioprinting and might help to comprehend and improve the outcome of cell-printing studies in the future.

  2. Gabor-domain optical coherence microscopy with integrated dual-axis MEMS scanner for fast 3D imaging and metrology

    NASA Astrophysics Data System (ADS)

    Canavesi, Cristina; Cogliati, Andrea; Hayes, Adam; Santhanam, Anand P.; Tankam, Patrice; Rolland, Jannick P.

    2015-10-01

    Fast, robust, nondestructive 3D imaging is needed for characterization of microscopic structures in industrial and clinical applications. A custom micro-electromechanical system (MEMS)-based 2D scanner system was developed to achieve 55 kHz A-scan acquisition in a Gabor-domain optical coherence microscopy (GD-OCM) instrument with a novel multilevel GPU architecture for high-speed imaging. GD-OCM yields high-definition volumetric imaging with dynamic depth of focusing through a bio-inspired liquid lens-based microscope design, which has no moving parts and is suitable for use in a manufacturing setting or in a medical environment. A dual-axis MEMS mirror was chosen to replace two single-axis galvanometer mirrors; as a result, the astigmatism caused by the mismatch between the optical pupil and the scanning location was eliminated and a 12x reduction in volume of the scanning system was achieved. Imaging at an invariant resolution of 2 μm was demonstrated throughout a volume of 1 × 1 × 0.6 mm3, acquired in less than 2 minutes. The MEMS-based scanner resulted in improved image quality, increased robustness and lighter weight of the system - all factors that are critical for on-field deployment. A custom integrated feedback system consisting of a laser diode and a position-sensing detector was developed to investigate the impact of the resonant frequency of the MEMS and the driving signal of the scanner on the movement of the mirror. Results on the metrology of manufactured materials and characterization of tissue samples with GD-OCM are presented.

  3. 3D printed electromagnetic transmission and electronic structures fabricated on a single platform using advanced process integration techniques

    NASA Astrophysics Data System (ADS)

    Deffenbaugh, Paul Issac

    3D printing has garnered immense attention from many fields including in-office rapid prototyping of mechanical parts, outer-space satellite replication, garage functional firearm manufacture, and NASA rocket engine component fabrication. 3D printing allows increased design flexibility in the fabrication of electronics, microwave circuits and wireless antennas and has reached a level of maturity which allows functional parts to be printed. Much more work is necessary in order to perfect the processes of 3D printed electronics especially in the area of automation. Chapter 1 shows several finished prototypes of 3D printed electronics as well as newly developed techniques in fabrication. Little is known about the RF and microwave properties and applications of the standard materials which have been developed for 3D printing. Measurement of a wide variety of materials over a broad spectrum of frequencies up to 10 GHz using a variety of well-established measurement methods is performed throughout chapter 2. Several types of high frequency RF transmission lines are fabricated and valuable model-matched data is gathered and provided in chapter 3 for future designers' use. Of particular note is a fully 3D printed stripline which was automatically fabricated in one process on one machine. Some core advantages of 3D printing RF/microwave components include rapid manufacturing of complex, dimensionally sensitive circuits (such as antennas and filters which are often iteratively tuned) and the ability to create new devices that cannot be made using standard fabrication techniques. Chapter 4 describes an exemplary fully 3D printed curved inverted-F antenna.

  4. Integral imaging-based large-scale full-color 3-D display of holographic data by using a commercial LCD panel.

    PubMed

    Dong, Xiao-Bin; Ai, Ling-Yu; Kim, Eun-Soo

    2016-02-22

    We propose a new type of integral imaging-based large-scale full-color three-dimensional (3-D) display of holographic data based on direct ray-optical conversion of holographic data into elemental images (EIs). In the proposed system, a 3-D scene is modeled as a collection of depth-sliced object images (DOIs), and three-color hologram patterns for that scene are generated by interfering each color DOI with a reference beam, and summing them all based on Fresnel convolution integrals. From these hologram patterns, full-color DOIs are reconstructed, and converted into EIs using a ray mapping-based direct pickup process. These EIs are then optically reconstructed to be a full-color 3-D scene with perspectives on the depth-priority integral imaging (DPII)-based 3-D display system employing a large-scale LCD panel. Experiments with a test video confirm the feasibility of the proposed system in the practical application fields of large-scale holographic 3-D displays.

  5. Fully integrated wearable sensor arrays for multiplexed in situ perspiration analysis

    SciTech Connect

    Gao, Wei; Emaminejad, Sam; Nyein, Hnin Yin Yin; Challa, Samyuktha; Chen, Kevin; Peck, Austin; Fahad, Hossain M.; Ota, Hiroki; Shiraki, Hiroshi; Kiriya, Daisuke; Lien, Der-Hsien; Brooks, George A.; Davis, Ronald W.; Javey, Ali

    2016-01-27

    We report that wearable sensor technologies are essential to the realization of personalized medicine through continuously monitoring an individual’s state of health. Sampling human sweat, which is rich in physiological information13, could enable non-invasive monitoring. Previously reported sweat-based and other noninvasive biosensors either can only monitor a single analyte at a time or lack on-site signal processing circuitry and sensor calibration mechanisms for accurate analysis of the physiological state14–18. Given the complexity of sweat secretion, simultaneous and multiplexed screening of target biomarkers is critical and requires full system integration to ensure the accuracy of measurements. Here we present a mechanically flexible and fully integrated (that is, no external analysis is needed) sensor array for multiplexed in situ perspiration analysis, which simultaneously and selectively measures sweat metabolites (such as glucose and lactate) and electrolytes (such as sodium and potassium ions), as well as the skin temperature (to calibrate the response of the sensors). Lastly, our work bridges the technological gap between signal transduction, conditioning (amplification and filtering), processing and wireless transmission in wearable biosensors by merging plasticbased sensors that interface with the skin with silicon integrated circuits consolidated on a flexible circuit board for complex signal processing.

  6. Fully integrated wearable sensor arrays for multiplexed in situ perspiration analysis

    DOE PAGES

    Gao, Wei; Emaminejad, Sam; Nyein, Hnin Yin Yin; ...

    2016-01-27

    We report that wearable sensor technologies are essential to the realization of personalized medicine through continuously monitoring an individual’s state of health. Sampling human sweat, which is rich in physiological information13, could enable non-invasive monitoring. Previously reported sweat-based and other noninvasive biosensors either can only monitor a single analyte at a time or lack on-site signal processing circuitry and sensor calibration mechanisms for accurate analysis of the physiological state14–18. Given the complexity of sweat secretion, simultaneous and multiplexed screening of target biomarkers is critical and requires full system integration to ensure the accuracy of measurements. Here we present a mechanicallymore » flexible and fully integrated (that is, no external analysis is needed) sensor array for multiplexed in situ perspiration analysis, which simultaneously and selectively measures sweat metabolites (such as glucose and lactate) and electrolytes (such as sodium and potassium ions), as well as the skin temperature (to calibrate the response of the sensors). Lastly, our work bridges the technological gap between signal transduction, conditioning (amplification and filtering), processing and wireless transmission in wearable biosensors by merging plasticbased sensors that interface with the skin with silicon integrated circuits consolidated on a flexible circuit board for complex signal processing.« less

  7. Integrating structure-from-motion photogrammetry with geospatial software as a novel technique for quantifying 3D ecological characteristics of coral reefs

    PubMed Central

    Delparte, D; Gates, RD; Takabayashi, M

    2015-01-01

    The structural complexity of coral reefs plays a major role in the biodiversity, productivity, and overall functionality of reef ecosystems. Conventional metrics with 2-dimensional properties are inadequate for characterization of reef structural complexity. A 3-dimensional (3D) approach can better quantify topography, rugosity and other structural characteristics that play an important role in the ecology of coral reef communities. Structure-from-Motion (SfM) is an emerging low-cost photogrammetric method for high-resolution 3D topographic reconstruction. This study utilized SfM 3D reconstruction software tools to create textured mesh models of a reef at French Frigate Shoals, an atoll in the Northwestern Hawaiian Islands. The reconstructed orthophoto and digital elevation model were then integrated with geospatial software in order to quantify metrics pertaining to 3D complexity. The resulting data provided high-resolution physical properties of coral colonies that were then combined with live cover to accurately characterize the reef as a living structure. The 3D reconstruction of reef structure and complexity can be integrated with other physiological and ecological parameters in future research to develop reliable ecosystem models and improve capacity to monitor changes in the health and function of coral reef ecosystems. PMID:26207190

  8. A 16-Channel Distributed-Feedback Laser Array with a Monolithic Integrated Arrayed Waveguide Grating Multiplexer for a Wavelength Division Multiplex-Passive Optical Network System Network

    NASA Astrophysics Data System (ADS)

    Zhao, Jian-Yi; Chen, Xin; Zhou, Ning; Huang, Xiao-Dong; Cao, Ming-De; Liu, Wen

    2014-07-01

    A 16-channel distributed-feedback (DFB) laser array with a monolithic integrated arrayed waveguide grating multiplexer for a wavelength division multiplex-passive optical network system is fabricated by using the butt-joint metal organic chemical vapor deposition technology and nanoimpirnt technology. The results show that the threshold current is about 20-30 mA at 25°C. The DFB laser side output power is about 16 mW with a 150 mA injection current. The lasing wavelength is from 1550 nm to 1575 nm covering a more than 25 nm range with 200 GHz channel space. A more than 55 dB sidemode suppression ratio is obtained.

  9. Experience with 3D integration technologies in the framework of the ATLAS pixel detector upgrade for the HL-LHC

    NASA Astrophysics Data System (ADS)

    Aruntinov, D.; Barbero, M.; Gonella, L.; Hemperek, T.; Hügging, F.; Krüger, H.; Wermes, N.; Breugnon, P.; Chantepie, B.; Clemens, J. C.; Fei, R.; Fougeron, D.; Godiot, S.; Pangaud, P.; Rozanov, A.; Garcia-Sciveres, M.; Mekkaoui, A.

    2013-12-01

    3D technologies are investigated for the upgrade of the ATLAS pixel detector at the HL-LHC. R&D focuses on both, IC design in 3D, as well as on post-processing 3D technologies such as Through Silicon Via (TSV). The first one uses a so-called via first technology, featuring the insertion of small aspect ratio TSV at the pixel level. As discussed in the paper, this technology can still present technical challenges for the industrial partners. The second one consists of etching the TSV via last. This technology is investigated to enable 4-side abuttable module concepts, using today's pixel detector technology. Both approaches are presented in this paper and results from first available prototypes are discussed.

  10. Integrated capillary electrophoresis microsystem for multiplex analysis of human respiratory viruses.

    PubMed

    Thaitrong, Numrin; Liu, Peng; Briese, Thomas; Lipkin, W Ian; Chiesl, Thomas N; Higa, Yukiko; Mathies, Richard A

    2010-12-15

    We developed a two-layer, four-channel polymerase chain reaction (PCR)-capillary electrophoresis microdevice that integrates nucleic acid amplification, sample cleanup and concentration, capillary electrophoretic separation, and detection for multiplex analysis of four human respiratory viral pathogens, influenza A, influenza B, coronavirus OC43, and human metapneumovirus. Biotinylated and fluorescently labeled double-stranded (ds) deoxyribonucleic acid (DNA) amplification products are generated in a 100 nL PCR reactor incorporating an integrated heater and a temperature sensor. After amplification, the products are captured and concentrated in a cross-linked acrylamide gel capture matrix copolymerized with acrydite-functionalized streptavidin-capture agents. Thermal dehybridization releases the fluorescently labeled DNA strand for capillary electrophoresis injection, separation, and detection. Using plasmid standards containing the viral genes of interest, each target can be detected starting from as few as 10 copies/reactor. When a two-step reverse transcription PCR amplification is employed, the device can detect ribonucleic acid (RNA) analogues of all four viral targets with detection limits in the range of 25-100 copies/reactor. The utility of the microdevice for analyzing samples from nasopharyngeal swabs is demonstrated. When size-based separation is combined with four-color detection, this platform provides excellent product discrimination, making it readily extendable to higher-order multiplex assays. This portable microsystem is also suitable for performing automated assays in point-of-care diagnostic applications.

  11. RADStation3G: a platform for cardiovascular image analysis integrating PACS, 3D+t visualization and grid computing.

    PubMed

    Perez, F; Huguet, J; Aguilar, R; Lara, L; Larrabide, I; Villa-Uriol, M C; López, J; Macho, J M; Rigo, A; Rosselló, J; Vera, S; Vivas, E; Fernàndez, J; Arbona, A; Frangi, A F; Herrero Jover, J; González Ballester, M A

    2013-06-01

    RADStation3G is a software platform for cardiovascular image analysis and surgery planning. It provides image visualization and management in 2D, 3D and 3D+t; data storage (images or operational results) in a PACS (using DICOM); and exploitation of patients' data such as images and pathologies. Further, it provides support for computationally expensive processes with grid technology. In this article we first introduce the platform and present a comparison with existing systems, according to the platform's modules (for cardiology, angiology, PACS archived enriched searching and grid computing), and then RADStation3G is described in detail.

  12. Data Multiplexing Network (DMN), phase 3: Equipment Operational Test and Evaluation (OT&E)/Integration test plan

    NASA Astrophysics Data System (ADS)

    Bell, Wayne

    1991-10-01

    The presented plan addresses the Operational Test and Evaluation (OT&E)/Integration testing of the Data Multiplexing Network (DMN) equipment in the current NAS environment. The approach and concept is to conduct integration tests with appropriate National Airspace System (NAS) systems to verify that NAS requirements and DMN specifications and Statistical Multiplexer requirements were satisfied. This will be accomplished by conducting unit level tests at the Federal Aviation Administration (FAA) Technical Center and extensive integration tests at the key test sites of Albuquerque, New Mexico; Denver, Colorado; and Minneapolis, Minnesota Air Route Traffic Control Centers (ARTCC). The DMN equipment being procured includes Deterministic Time Division Multiplexing (DTDM) Commercial Off-The-Shelf (COTS) equipment and the NON-COTS equipment (a/b switch and clock box). This plan describes the test program for the DTDM COTS equipment.

  13. DNA Assembly in 3D Printed Fluidics

    PubMed Central

    Patrick, William G.; Nielsen, Alec A. K.; Keating, Steven J.; Levy, Taylor J.; Wang, Che-Wei; Rivera, Jaime J.; Mondragón-Palomino, Octavio; Carr, Peter A.; Voigt, Christopher A.; Oxman, Neri; Kong, David S.

    2015-01-01

    The process of connecting genetic parts—DNA assembly—is a foundational technology for synthetic biology. Microfluidics present an attractive solution for minimizing use of costly reagents, enabling multiplexed reactions, and automating protocols by integrating multiple protocol steps. However, microfluidics fabrication and operation can be expensive and requires expertise, limiting access to the technology. With advances in commodity digital fabrication tools, it is now possible to directly print fluidic devices and supporting hardware. 3D printed micro- and millifluidic devices are inexpensive, easy to make and quick to produce. We demonstrate Golden Gate DNA assembly in 3D-printed fluidics with reaction volumes as small as 490 nL, channel widths as fine as 220 microns, and per unit part costs ranging from $0.61 to $5.71. A 3D-printed syringe pump with an accompanying programmable software interface was designed and fabricated to operate the devices. Quick turnaround and inexpensive materials allowed for rapid exploration of device parameters, demonstrating a manufacturing paradigm for designing and fabricating hardware for synthetic biology. PMID:26716448

  14. A rapid, straightforward, and print house compatible mass fabrication method for integrating 3D paper-based microfluidics.

    PubMed

    Xiao, Liangpin; Liu, Xianming; Zhong, Runtao; Zhang, Kaiqing; Zhang, Xiaodi; Zhou, Xiaomian; Lin, Bingcheng; Du, Yuguang

    2013-11-01

    Three-dimensional (3D) paper-based microfluidics, which is featured with high performance and speedy determination, promise to carry out multistep sample pretreatment and orderly chemical reaction, which have been used for medical diagnosis, cell culture, environment determination, and so on with broad market prospect. However, there are some drawbacks in the existing fabrication methods for 3D paper-based microfluidics, such as, cumbersome and time-consuming device assembly; expensive and difficult process for manufacture; contamination caused by organic reagents from their fabrication process. Here, we present a simple printing-bookbinding method for mass fabricating 3D paper-based microfluidics. This approach involves two main steps: (i) wax-printing, (ii) bookbinding. We tested the delivery capability, diffusion rate, homogeneity and demonstrated the applicability of the device to chemical analysis by nitrite colorimetric assays. The described method is rapid (<30 s), cheap, easy to manipulate, and compatible with the flat stitching method that is common in a print house, making itself an ideal scheme for large-scale production of 3D paper-based microfluidics.

  15. An integrated control panel utilizing a programmable varistor-multiplexed dichroic liquid crystal display

    NASA Technical Reports Server (NTRS)

    Whitton, I. J.

    1981-01-01

    Due to the conflicting demands of modern aircraft for increased systems/sensors and the decrease in cockpit panel size, weight, volume, and power, conventional discrete/dedicated methods of display and control are fast becoming obsolete. A means is sought to integrate the control and display into multifunctional programmable devices, thus giving the ability to increase system functions and yet conserve panel space. A potential solution to the control portion of the problem has come to be known as the Integrated Control Panel (ICP) approach. Flat panel display technology and controls using programmable flat panel displays with transparent capacitive touch control overlays offer the largest potential advantages. The flat panel display made of varistor-multiplexed dichroic liquid crystal (LCD) developed by GE in recent years appears to offer the ideal monochrome solution.

  16. iRegNet3D: three-dimensional integrated regulatory network for the genomic analysis of coding and non-coding disease mutations.

    PubMed

    Liang, Siqi; Tippens, Nathaniel D; Zhou, Yaoda; Mort, Matthew; Stenson, Peter D; Cooper, David N; Yu, Haiyuan

    2017-01-18

    The mechanistic details of most disease-causing mutations remain poorly explored within the context of regulatory networks. We present a high-resolution three-dimensional integrated regulatory network (iRegNet3D) in the form of a web tool, where we resolve the interfaces of all known transcription factor (TF)-TF, TF-DNA and chromatin-chromatin interactions for the analysis of both coding and non-coding disease-associated mutations to obtain mechanistic insights into their functional impact. Using iRegNet3D, we find that disease-associated mutations may perturb the regulatory network through diverse mechanisms including chromatin looping. iRegNet3D promises to be an indispensable tool in large-scale sequencing and disease association studies.

  17. Integrated VR platform for 3D and image-based models: a step toward interactive image-based virtual environments

    NASA Astrophysics Data System (ADS)

    Yoon, Jayoung; Kim, Gerard J.

    2003-04-01

    Traditionally, three dimension models have been used for building virtual worlds, and a data structure called the "scene graph" is often employed to organize these 3D objects in the virtual space. On the other hand, image-based rendering has recently been suggested as a probable alternative VR platform for its photo-realism, however, due to limited interactivity, it has only been used for simple navigation systems. To combine the merits of these two approaches to object/scene representations, this paper proposes for a scene graph structure in which both 3D models and various image-based scenes/objects can be defined, traversed, and rendered together. In fact, as suggested by Shade et al., these different representations can be used as different LOD's for a given object. For instance, an object might be rendered using a 3D model at close range, a billboard at an intermediate range, and as part of an environment map at far range. The ultimate objective of this mixed platform is to breath more interactivity into the image based rendered VE's by employing 3D models as well. There are several technical challenges in devising such a platform: designing scene graph nodes for various types of image based techniques, establishing criteria for LOD/representation selection, handling their transitions, implementing appropriate interaction schemes, and correctly rendering the overall scene. Currently, we have extended the scene graph structure of the Sense8's WorldToolKit, to accommodate new node types for environment maps billboards, moving textures and sprites, "Tour-into-the-Picture" structure, and view interpolated objects. As for choosing the right LOD level, the usual viewing distance and image space criteria are used, however, the switching between the image and 3D model occurs at a distance from the user where the user starts to perceive the object's internal depth. Also, during interaction, regardless of the viewing distance, a 3D representation would be used, it if

  18. Integrated quantum dot barcode smartphone optical device for wireless multiplexed diagnosis of infected patients.

    PubMed

    Ming, Kevin; Kim, Jisung; Biondi, Mia J; Syed, Abdullah; Chen, Kun; Lam, Albert; Ostrowski, Mario; Rebbapragada, Anu; Feld, Jordan J; Chan, Warren C W

    2015-03-24

    Inorganic nanoparticles are ideal precursors for engineering barcodes for rapidly detecting diseases. Despite advances in the chemical design of these barcodes, they have not advanced to clinical use because they lack sensitivity and are not cost-effective due to requirement of a large read-out system. Here we combined recent advances in quantum dot barcode technology with smartphones and isothermal amplification to engineer a simple and low-cost chip-based wireless multiplex diagnostic device. We characterized the analytical performance of this device and demonstrated that the device is capable of detecting down to 1000 viral genetic copies per milliliter, and this enabled the diagnosis of patients infected with HIV or hepatitis B. More importantly, the barcoding enabled us to detect multiple infectious pathogens simultaneously, in a single test, in less than 1 h. This multiplexing capability of the device enables the diagnosis of infections that are difficult to differentiate clinically due to common symptoms such as a fever or rash. The integration of quantum dot barcoding technology with a smartphone reader provides a capacity for global surveillance of infectious diseases and the potential to accelerate knowledge exchange transfer of emerging or exigent disease threats with healthcare and military organizations in real time.

  19. An Integrated Quantum Dot Barcode Smartphone Optical Device for Wireless Multiplexed Diagnosis of Infected Patients

    NASA Astrophysics Data System (ADS)

    Ming, Kevin

    Integrating mobile-cellular devices with multiplex molecular diagnostics can potentially provide the most powerful platform for tracking, managing and preventing the transmission of infectious diseases. With over 6.9 billion subscriptions globally, handheld mobile-cellular devices can be programmed to spatially map, temporally track, and transmit information on infections over wide geographical space and boundaries. Current cell phone diagnostic technologies have poor limit of detection, dynamic range, and cannot detect multiple pathogen targets simultaneously, limiting their utility to single infections with high load. Here we combined recent advances in quantum dot barcode technology for molecular detection with smartphones to engineer a simple and low-cost chip-based wireless multiplex diagnostic device. We validated our device using a variety of synthetic genomic targets for the respiratory virus and blood-borne pathogens, and demonstrated that it could detect clinical samples after simple amplification. More importantly, we confirmed that the device is capable of detecting patients infected with a single or multiple infectious pathogens (e.g., HIV and hepatitis B) in a single test. This device advances the capacity for global surveillance of infectious diseases and has the potential to accelerate knowledge exchange-transfer of emerging or exigent disease threats with healthcare and military organizations in real-time.

  20. Fully integrated wearable sensor arrays for multiplexed in situ perspiration analysis

    NASA Astrophysics Data System (ADS)

    Gao, Wei; Emaminejad, Sam; Nyein, Hnin Yin Yin; Challa, Samyuktha; Chen, Kevin; Peck, Austin; Fahad, Hossain M.; Ota, Hiroki; Shiraki, Hiroshi; Kiriya, Daisuke; Lien, Der-Hsien; Brooks, George A.; Davis, Ronald W.; Javey, Ali

    2016-01-01

    Wearable sensor technologies are essential to the realization of personalized medicine through continuously monitoring an individual’s state of health. Sampling human sweat, which is rich in physiological information, could enable non-invasive monitoring. Previously reported sweat-based and other non-invasive biosensors either can only monitor a single analyte at a time or lack on-site signal processing circuitry and sensor calibration mechanisms for accurate analysis of the physiological state. Given the complexity of sweat secretion, simultaneous and multiplexed screening of target biomarkers is critical and requires full system integration to ensure the accuracy of measurements. Here we present a mechanically flexible and fully integrated (that is, no external analysis is needed) sensor array for multiplexed in situ perspiration analysis, which simultaneously and selectively measures sweat metabolites (such as glucose and lactate) and electrolytes (such as sodium and potassium ions), as well as the skin temperature (to calibrate the response of the sensors). Our work bridges the technological gap between signal transduction, conditioning (amplification and filtering), processing and wireless transmission in wearable biosensors by merging plastic-based sensors that interface with the skin with silicon integrated circuits consolidated on a flexible circuit board for complex signal processing. This application could not have been realized using either of these technologies alone owing to their respective inherent limitations. The wearable system is used to measure the detailed sweat profile of human subjects engaged in prolonged indoor and outdoor physical activities, and to make a real-time assessment of the physiological state of the subjects. This platform enables a wide range of personalized diagnostic and physiological monitoring applications.

  1. Fully integrated wearable sensor arrays for multiplexed in situ perspiration analysis.

    PubMed

    Gao, Wei; Emaminejad, Sam; Nyein, Hnin Yin Yin; Challa, Samyuktha; Chen, Kevin; Peck, Austin; Fahad, Hossain M; Ota, Hiroki; Shiraki, Hiroshi; Kiriya, Daisuke; Lien, Der-Hsien; Brooks, George A; Davis, Ronald W; Javey, Ali

    2016-01-28

    Wearable sensor technologies are essential to the realization of personalized medicine through continuously monitoring an individual's state of health. Sampling human sweat, which is rich in physiological information, could enable non-invasive monitoring. Previously reported sweat-based and other non-invasive biosensors either can only monitor a single analyte at a time or lack on-site signal processing circuitry and sensor calibration mechanisms for accurate analysis of the physiological state. Given the complexity of sweat secretion, simultaneous and multiplexed screening of target biomarkers is critical and requires full system integration to ensure the accuracy of measurements. Here we present a mechanically flexible and fully integrated (that is, no external analysis is needed) sensor array for multiplexed in situ perspiration analysis, which simultaneously and selectively measures sweat metabolites (such as glucose and lactate) and electrolytes (such as sodium and potassium ions), as well as the skin temperature (to calibrate the response of the sensors). Our work bridges the technological gap between signal transduction, conditioning (amplification and filtering), processing and wireless transmission in wearable biosensors by merging plastic-based sensors that interface with the skin with silicon integrated circuits consolidated on a flexible circuit board for complex signal processing. This application could not have been realized using either of these technologies alone owing to their respective inherent limitations. The wearable system is used to measure the detailed sweat profile of human subjects engaged in prolonged indoor and outdoor physical activities, and to make a real-time assessment of the physiological state of the subjects. This platform enables a wide range of personalized diagnostic and physiological monitoring applications.

  2. Fully integrated wearable sensor arrays for multiplexed in situ perspiration analysis

    PubMed Central

    Nyein, Hnin Yin Yin; Challa, Samyuktha; Chen, Kevin; Peck, Austin; Fahad, Hossain M.; Ota, Hiroki; Shiraki, Hiroshi; Kiriya, Daisuke; Lien, Der-Hsien; Brooks, George A.; Davis, Ronald W.; Javey, Ali

    2016-01-01

    Wearable sensor technologies are essential to the realization of personalized medicine through continuously monitoring an individual's state of health1–12. Sampling human sweat, which is rich in physiological information13, could enable non-invasive monitoring. Previously reported sweat-based and other non-invasive biosensors either can only monitor a single analyte at a time or lack on-site signal processing circuitry and sensor calibration mechanisms for accurate analysis of the physiological state14–18. Given the complexity of sweat secretion, simultaneous and multiplexed screening of target biomarkers is critical and requires full system integration to ensure the accuracy of measurements. Here we present a mechanically flexible and fully integrated (that is, no external analysis is needed) sensor array for multiplexed in situ perspiration analysis, which simultaneously and selectively measures sweat metabolites (such as glucose and lactate) and electrolytes (such as sodium and potassium ions), as well as the skin temperature (to calibrate the response of the sensors). Our work bridges the technological gap between signal transduction, conditioning (amplification and filtering), processing and wireless transmission in wearable biosensors by merging plastic-based sensors that interface with the skin with silicon integrated circuits consolidated on a flexible circuit board for complex signal processing. This application could not have been realized using either of these technologies alone owing to their respective inherent limitations. The wearable system is used to measure the detailed sweat profile of human subjects engaged in prolonged indoor and outdoor physical activities, and to make a real-time assessment of the physiological state of the subjects. This platform enables a wide range of personalized diagnostic and physiological monitoring applications. PMID:26819044

  3. A strategy for integrating essential 3D microphysiological systems of human organs for realistic anti-cancer drug screening

    PubMed Central

    Heylman, Christopher; Sobrino, Agua; Shirure, Venktesh S.; Hughes, Christopher C.W.; George, Steven C.

    2014-01-01

    Cancer is one of the leading causes of morbidity and mortality around the world. Despite some success, traditional anti-cancer drugs developed to reduce tumor growth face important limitations primarily due to undesirable bone marrow and cardiovascular toxicity. Many drugs showing promise in preclinical trials fail during clinical development, suggesting that the available in vitro and animal models are poor predictors of drug efficacy and toxicity in humans. Hence, there exists a great need for developing novel platforms that more accurately mimic the biology of human organs, and thus provide a reliable model for high-throughput drug screening. 3D microphysiological systems can utilize induced pluripotent stem (iPS) cell technology, tissue engineering, and microfabrication techniques to develop tissue models of human tumors, cardiac muscle, and bone marrow on the order of 1 mm3 in size. A functional network of human capillaries and microvessels to overcome diffusion limitations in nutrient delivery and waste removal can also nourish the 3D microphysiological tissues. Importantly, the 3D microphysiological tissues are grown on optically clear platforms that offer non-invasive and non-destructive image acquisition with sub-cellular resolution in real time. Such systems offer a new paradigm for high-throughput drug screening, and will significantly improve the efficiency of identifying new drugs for cancer treatment, that minimize cardiac and bone marrow toxicity. PMID:24740872

  4. Integrating sediment biogeochemistry into 3D oceanic models: A study of benthic-pelagic coupling in the Black Sea

    NASA Astrophysics Data System (ADS)

    Capet, Arthur; Meysman, Filip J. R.; Akoumianaki, Ioanna; Soetaert, Karline; Grégoire, Marilaure

    2016-05-01

    Three-dimensional (3D) ecosystem models of shelf environments should properly account for the biogeochemical cycling within the sea floor. However, a full and explicit representation of sediment biogeochemistry into 3D ocean models is computationally demanding. Here, we describe a simplified approach to include benthic processes in 3D ocean models, which includes a parameterization of the different pathways for organic matter mineralization and allows for organic matter remobilization by bottom currents and waves. This efficient approach enables decadal simulations that resolve the inertial contribution of the sea floor to the biogeochemical cycling in shelf environments. The model was implemented to analyze the benthic-pelagic coupling in the northwestern shelf of the Black Sea. Three distinct biogeochemical provinces were identified on the basis of fluxes and rates associated with benthic-pelagic coupling. Our model simulations suitably capture the seasonal variability of in situ flux data as well as their regional variation, which stresses the importance of incorporating temporally varying sediment biogeochemistry and resuspension/redeposition cycles in shelf ecosystem models.

  5. Engineering Multi-scale Electrospun Structure for Integration into Architected 3-D Nanofibers for Cimex Annihilation: Fabrication and Mechanism Study

    NASA Astrophysics Data System (ADS)

    He, Shan; Zhang, Linxi; Liu, Ying; Rafailovich, Miriam; Garcia CenterPolymers at Engineered Interfaces Team

    In this study, engineered electrospun scaffolds with fibers oriented with designed curvature in three dimensions (3D) including the looped structure were developed based on the principle of electrostatic repulsion. Here we illustrate that 3D electrospun recycled polystyrene fibers could closely mimic the unique architectures of multi-direction and multi-layer nano-spiderweb. In contrast to virgin PS, the recycled PS (Dart Styrofoam) are known to contain zinc stearate which acts as a surfactant resulting in higher electrical charge and larger fiber curvature, hence, lower modulus. The surfactant, which is known to decrease the surface tension, may have also been effective at decreasing the confinement of the PS, where chain stretching was shown to occur, in response to the high surface tension at the air interface. Three dimensional flexible architecture with complex structures are shown to be necessary in order to block the motion of Cimex lectularius. Here we show how an engineered electrospun network of surfactant modified polymer fibers with calculated dimensions can be used to immobilize the insects. The mechanical response of the fibers has to be specifically tailored so that it is elastically deformed, without fracturing or flowing. Carefully controlling and tailoring the electrospinning parameters we can now utilize architected 3D nanofiber to create an environmental-friendly Cimex immobilization device which can lead to annihilation solution for all the other harmful insects.

  6. Genome3D: A viewer-model framework for integrating and visualizing multi-scale epigenomic information within a three-dimensional genome

    PubMed Central

    2010-01-01

    Background New technologies are enabling the measurement of many types of genomic and epigenomic information at scales ranging from the atomic to nuclear. Much of this new data is increasingly structural in nature, and is often difficult to coordinate with other data sets. There is a legitimate need for integrating and visualizing these disparate data sets to reveal structural relationships not apparent when looking at these data in isolation. Results We have applied object-oriented technology to develop a downloadable visualization tool, Genome3D, for integrating and displaying epigenomic data within a prescribed three-dimensional physical model of the human genome. In order to integrate and visualize large volume of data, novel statistical and mathematical approaches have been developed to reduce the size of the data. To our knowledge, this is the first such tool developed that can visualize human genome in three-dimension. We describe here the major features of Genome3D and discuss our multi-scale data framework using a representative basic physical model. We then demonstrate many of the issues and benefits of multi-resolution data integration. Conclusions Genome3D is a software visualization tool that explores a wide range of structural genomic and epigenetic data. Data from various sources of differing scales can be integrated within a hierarchical framework that is easily adapted to new developments concerning the structure of the physical genome. In addition, our tool has a simple annotation mechanism to incorporate non-structural information. Genome3D is unique is its ability to manipulate large amounts of multi-resolution data from diverse sources to uncover complex and new structural relationships within the genome. PMID:20813045

  7. Wavelength-division multiplexed optical integrated circuit with vertical diffraction grating

    NASA Technical Reports Server (NTRS)

    Lang, Robert J. (Inventor); Forouhar, Siamak (Inventor)

    1994-01-01

    A semiconductor optical integrated circuit for wave division multiplexing has a semiconductor waveguide layer, a succession of diffraction grating points in the waveguide layer along a predetermined diffraction grating contour, a semiconductor diode array in the waveguide layer having plural optical ports facing the succession of diffraction grating points along a first direction, respective semiconductor diodes in the array corresponding to respective ones of a predetermined succession of wavelengths, an optical fiber having one end thereof terminated at the waveguide layer, the one end of the optical fiber facing the succession of diffraction grating points along a second direction, wherein the diffraction grating points are spatially distributed along the predetermined contour in such a manner that the succession of diffraction grating points diffracts light of respective ones of the succession of wavelengths between the one end of the optical fiber and corresponding ones of the optical ports.

  8. TransCAIP: A Live 3D TV system using a camera array and an integral photography display with interactive control of viewing parameters.

    PubMed

    Taguchi, Yuichi; Koike, Takafumi; Takahashi, Keita; Naemura, Takeshi

    2009-01-01

    The system described in this paper provides a real-time 3D visual experience by using an array of 64 video cameras and an integral photography display with 60 viewing directions. The live 3D scene in front of the camera array is reproduced by the full-color, full-parallax autostereoscopic display with interactive control of viewing parameters. The main technical challenge is fast and flexible conversion of the data from the 64 multicamera images to the integral photography format. Based on image-based rendering techniques, our conversion method first renders 60 novel images corresponding to the viewing directions of the display, and then arranges the rendered pixels to produce an integral photography image. For real-time processing on a single PC, all the conversion processes are implemented on a GPU with GPGPU techniques. The conversion method also allows a user to interactively control viewing parameters of the displayed image for reproducing the dynamic 3D scene with desirable parameters. This control is performed as a software process, without reconfiguring the hardware system, by changing the rendering parameters such as the convergence point of the rendering cameras and the interval between the viewpoints of the rendering cameras.

  9. Integration of 3D 1H-magnetic resonance spectroscopy data into neuronavigation systems for tumor biopsies

    NASA Astrophysics Data System (ADS)

    Kanberoglu, Berkay; Moore, Nina Z.; Frakes, David; Karam, Lina J.; Debbins, Josef P.; Preul, Mark C.

    2013-03-01

    Many important applications in clinical medicine can benefit from the fusion of spectroscopy data with anatomical images. For example, the correlation of metabolite profiles with specific regions of interest in anatomical tumor images can be useful in characterizing and treating heterogeneous tumors that appear structurally homogeneous. Such applications can build on the correlation of data from in-vivo Proton Magnetic Resonance Spectroscopy Imaging (1HMRSI) with data from genetic and ex-vivo Nuclear Magnetic Resonance spectroscopy. To establish that correlation, tissue samples must be neurosurgically extracted from specifically identified locations with high accuracy. Toward that end, this paper presents new neuronavigation technology that enhances current clinical capabilities in the context of neurosurgical planning and execution. The proposed methods improve upon the current state-of-the-art in neuronavigation through the use of detailed three dimensional (3D) 1H-MRSI data. MRSI spectra are processed and analyzed, and specific voxels are selected based on their chemical contents. 3D neuronavigation overlays are then generated and applied to anatomical image data in the operating room. Without such technology, neurosurgeons must rely on memory and other qualitative resources alone for guidance in accessing specific MRSI-identified voxels. In contrast, MRSI-based overlays provide quantitative visual cues and location information during neurosurgery. The proposed methods enable a progressive new form of online MRSI-guided neuronavigation that we demonstrate in this study through phantom validation and clinical application.

  10. Silicon on-chip 1D photonic crystal nanobeam bandstop filters for the parallel multiplexing of ultra-compact integrated sensor array.

    PubMed

    Yang, Daquan; Wang, Chuan; Ji, Yuefeng

    2016-07-25

    We propose a novel multiplexed ultra-compact high-sensitivity one-dimensional (1D) photonic crystal (PC) nanobeam cavity sensor array on a monolithic silicon chip, referred to as Parallel Integrated 1D PC Nanobeam Cavity Sensor Array (PI-1DPC-NCSA). The performance of the device is investigated numerically with three-dimensional finite-difference time-domain (3D-FDTD) technique. The PI-1DPC-NCSA consists of multiple parallel-connected channels of integrated 1D PC nanobeam cavities/waveguides with gap separations. On each channel, by connecting two additional 1D PC nanobeam bandstop filters (1DPC-NBFs) to a 1D PC nanobeam cavity sensor (1DPC-NCS) in series, a transmission spectrum with a single targeted resonance is achieved for the purpose of multiplexed sensing applications. While the other spurious resonances are filtered out by the stop-band of 1DPC-NBF, multiple 1DPC-NCSs at different resonances can be connected in parallel without spectrum overlap. Furthermore, in order for all 1DPC-NCSs to be integrated into microarrays and to be interrogated simultaneously with a single input/output port, all channels are then connected in parallel by using a 1 × n taper-type equal power splitter and a n × 1 S-type power combiner in the input port and output port, respectively (n is the channel number). The concept model of PI-1DPC-NCSA is displayed with a 3-parallel-channel 1DPC-NCSs array containing series-connected 1DPC-NBFs. The bulk refractive index sensitivities as high as 112.6nm/RIU, 121.7nm/RIU, and 148.5nm/RIU are obtained (RIU = Refractive Index Unit). In particular, the footprint of the 3-parallel-channel PI-1DPC-NCSA is 4.5μm × 50μm (width × length), decreased by more than three orders of magnitude compared to 2D PC integrated sensor arrays. Thus, this is a promising platform for realizing ultra-compact lab-on-a-chip applications with high integration density and high parallel-multiplexing capabilities.

  11. Development and application of a ray-tracing code integrating with 3D equilibrium mapping in LHD ECH experiments

    NASA Astrophysics Data System (ADS)

    Tsujimura, T., Ii; Kubo, S.; Takahashi, H.; Makino, R.; Seki, R.; Yoshimura, Y.; Igami, H.; Shimozuma, T.; Ida, K.; Suzuki, C.; Emoto, M.; Yokoyama, M.; Kobayashi, T.; Moon, C.; Nagaoka, K.; Osakabe, M.; Kobayashi, S.; Ito, S.; Mizuno, Y.; Okada, K.; Ejiri, A.; Mutoh, T.

    2015-11-01

    The central electron temperature has successfully reached up to 7.5 keV in large helical device (LHD) plasmas with a central high-ion temperature of 5 keV and a central electron density of 1.3× {{10}19} m-3. This result was obtained by heating with a newly-installed 154 GHz gyrotron and also the optimisation of injection geometry in electron cyclotron heating (ECH). The optimisation was carried out by using the ray-tracing code ‘LHDGauss’, which was upgraded to include the rapid post-processing three-dimensional (3D) equilibrium mapping obtained from experiments. For ray-tracing calculations, LHDGauss can automatically read the relevant data registered in the LHD database after a discharge, such as ECH injection settings (e.g. Gaussian beam parameters, target positions, polarisation and ECH power) and Thomson scattering diagnostic data along with the 3D equilibrium mapping data. The equilibrium map of the electron density and temperature profiles are then extrapolated into the region outside the last closed flux surface. Mode purity, or the ratio between the ordinary mode and the extraordinary mode, is obtained by calculating the 1D full-wave equation along the direction of the rays from the antenna to the absorption target point. Using the virtual magnetic flux surfaces, the effects of the modelled density profiles and the magnetic shear at the peripheral region with a given polarisation are taken into account. Power deposition profiles calculated for each Thomson scattering measurement timing are registered in the LHD database. The adjustment of the injection settings for the desired deposition profile from the feedback provided on a shot-by-shot basis resulted in an effective experimental procedure.

  12. Online process monitoring at quasi-simultaneous laser transmission welding using a 3D-scanner with integrated pyrometer

    NASA Astrophysics Data System (ADS)

    Schmailzl, A.; Steger, S.; Dostalek, M.; Hierl, S.

    2016-03-01

    Quasi-simultaneous laser transmission welding is a well-known joining technique for thermoplastics and mainly used in the automotive as well as in the medical industry. For process control usually the so called set-path monitoring is used, where the weld is specified as "good" if the irradiation time is inside a defined confidence interval. However, the detection of small-sized gaps or thermal damaged zones is not possible with this technique. The analyzation of the weld seam temperature during welding offers the possibility to overcome this problem. In this approach a 3D-scanner is used instead of a scanner with flat-field optic. By using a pyrometer in combination with a 3D-scanner no color-corrected optic is needed in order to provide that laser- and detection-spot are concentric. Experimental studies on polyethylene T-joints have shown that the quality of the signal is adequate, despite the use of an optical setup with a long working distance and a small optical aperture. The effects on temperature are studied for defects like a gap in the joining zone. Therefore a notch was milled into the absorbent polymer. In case of producing housings for electronic parts the effect of an electrical wire between the joining partners is also investigated. Both defects can be identified by a local temperature deviation even at a feed rate of four meters per second. Furthermore a strategy for signal-processing is demonstrated. By this, remaining defects can be identified. Consequently an online detection of local defects is possible, which makes a dynamic process control feasible.

  13. Real-time 3D display system based on computer-generated integral imaging technique using enhanced ISPP for hexagonal lens array.

    PubMed

    Kim, Do-Hyeong; Erdenebat, Munkh-Uchral; Kwon, Ki-Chul; Jeong, Ji-Seong; Lee, Jae-Won; Kim, Kyung-Ah; Kim, Nam; Yoo, Kwan-Hee

    2013-12-01

    This paper proposes an open computer language (OpenCL) parallel processing method to generate the elemental image arrays (EIAs) for hexagonal lens array from a three-dimensional (3D) object such as a volume data. Hexagonal lens array has a higher fill factor compared to the rectangular lens array case; however, each pixel of an elemental image should be determined to belong to the single hexagonal lens. Therefore, generation for the entire EIA requires very large computations. The proposed method reduces processing time for the EIAs for a given hexagonal lens array. By using the proposed image space parallel processing (ISPP) method, it can enhance the processing speed that generates the 3D display of real-time interactive integral imaging for hexagonal lens array. In our experiment, we implemented the EIAs for hexagonal lens array in real-time and obtained a good processing time for a large of volume data for multiple cases of lens arrays.

  14. Multi-sourced, 3D geometric characterization of volcanogenic karst features: Integrating lidar, sonar, and geophysical datasets (Invited)

    NASA Astrophysics Data System (ADS)

    Sharp, J. M.; Gary, M. O.; Reyes, R.; Halihan, T.; Fairfield, N.; Stone, W. C.

    2009-12-01

    Karstic aquifers can form very complex hydrogeological systems and 3-D mapping has been difficult, but Lidar, phased array sonar, and improved earth resistivity techniques show promise in this and in linking metadata to models. Zacatón, perhaps the Earth’s deepest cenote, has a sub-aquatic void space exceeding 7.5 x 106 cubic m3. It is the focus of this study which has created detailed 3D maps of the system. These maps include data from above and beneath the the water table and within the rock matrix to document the extent of the immense karst features and to interpret the geologic processes that formed them. Phase 1 used high resolution (20 mm) Lidar scanning of surficial features of four large cenotes. Scan locations, selected to achieve full feature coverage once registered, were established atop surface benchmarks with UTM coordinates established using GPS and Total Stations. The combined datasets form a geo-registered mesh of surface features down to water level in the cenotes. Phase 2 conducted subsurface imaging using Earth Resistivity Imaging (ERI) geophysics. ERI identified void spaces isolated from open flow conduits. A unique travertine morphology exists in which some cenotes are dry or contain shallow lakes with flat travertine floors; some water-filled cenotes have flat floors without the cone of collapse material; and some have collapse cones. We hypothesize that the floors may have large water-filled voids beneath them. Three separate flat travertine caps were imaged: 1) La Pilita, which is partially open, exposing cap structure over a deep water-filled shaft; 2) Poza Seca, which is dry and vegetated; and 3) Tule, which contains a shallow (<1 m) lake. A fourth line was run adjacent to cenote Verde. La Pilita ERI, verified by SCUBA, documented the existence of large water-filled void zones ERI at Poza Seca showed a thin cap overlying a conductive zone extending to at least 25 m depth beneath the cap with no lower boundary of this zone evident

  15. FFT integration of instantaneous 3D pressure gradient fields measured by Lagrangian particle tracking in turbulent flows

    NASA Astrophysics Data System (ADS)

    Huhn, F.; Schanz, D.; Gesemann, S.; Schröder, A.

    2016-09-01

    Pressure gradient fields in unsteady flows can be estimated through flow measurements of the material acceleration in the fluid and the assumption of the governing momentum equation. In order to derive pressure from its gradient, almost exclusively two numerical methods have been used to spatially integrate the pressure gradient until now: first, direct path integration in the spatial domain, and second, the solution of the Poisson equation for pressure. Instead, we propose an alternative third method that integrates the pressure gradient field in Fourier space. Using a FFT function, the method is fast and easy to implement in programming languages for scientific computing. We demonstrate the accuracy of the integration scheme on a synthetic pressure field and apply it to an experimental example based on time-resolved material acceleration data from high-resolution Lagrangian particle tracking with the Shake-The-Box method.

  16. Multiplex genomic walking: Integration of the wet lab and computer lab into a single prototyping environment

    SciTech Connect

    Gillevet, P.M.

    1993-12-31

    The authors are presently sequencing the entire genome of Mycoplasma capricolum, one of the smallest of free living organisms by a Multiplex Genomic Walking strategy. This technique involves the repetitive hybridization of sequencing membranes with oligonucleotide probes to acquire sequence data in discrete steps along the genome. The technique allows one to walk a genome in a directed manner eliminating the problems associated with random shotgun assembly. Furthermore, the repetitive stripping and hybridization process is relatively simple to reproduce and has the potential to be easily automated. The Genetic Data Environment (GDE), an X Windows based Graphic User Interface has allowed the seamless integration of a core multiple sequence editor with pre-existing external sequence analysis programs and internally developed programs into a single prototypic environment. This system has facilitated linkage of the 9 Harvard Genome Lab`s internal database and automated data control systems into one Graphic User Interface which can handle the archiving and analysis of both random fluorescent sequencing data and genomic walking data from the Mycoplasma project. Finally, it has facilitated the integration of the Genomic sequence data into a PROLOG database environment for the comparative analysis of Mycoplasma capricolum and other organisms.

  17. Integrated Design and Simulation of Tunable, Multi-State Structures Fabricated Monolithically with Multi-Material 3D Printing

    PubMed Central

    Chen, Tian; Mueller, Jochen; Shea, Kristina

    2017-01-01

    Multi-material 3D printing has created new opportunities for fabricating deployable structures. We design reversible, deployable structures that are fabricated flat, have defined load bearing capacity, and multiple, predictable activated geometries. These structures are designed with a hierarchical framework where the proposed bistable actuator serves as the base building block. The actuator is designed to maximise its stroke length, with the expansion ratio approaching one when serially connected. The activation force of the actuator is parameterised through its joint material and joint length. Simulation and experimental results show that the bistability triggering force can be tuned between 0.5 and 5.0 N. Incorporating this bistable actuator, the first group of hierarchical designs demonstrate the deployment of space frame structures with a tetrahedron module consisting of three active edges, each containing four serially connected actuators. The second group shows the design of flat structures that assume either positive or negative Gaussian curvature once activated. By flipping the initial configuration of the unit actuators, structures such as a dome and an enclosure are demonstrated. A modified Dynamic Relaxation method is used to simulate all possible geometries of the hierarchical structures. Measured geometries differ by less than 5% compared to simulation results. PMID:28361891

  18. Deep nuclear invaginations are linked to cytoskeletal filaments - integrated bioimaging of epithelial cells in 3D culture.

    PubMed

    Jorgens, Danielle M; Inman, Jamie L; Wojcik, Michal; Robertson, Claire; Palsdottir, Hildur; Tsai, Wen-Ting; Huang, Haina; Bruni-Cardoso, Alexandre; López, Claudia S; Bissell, Mina J; Xu, Ke; Auer, Manfred

    2017-01-01

    The importance of context in regulation of gene expression is now an accepted principle; yet the mechanism by which the microenvironment communicates with the nucleus and chromatin in healthy tissues is poorly understood. A functional role for nuclear and cytoskeletal architecture is suggested by the phenotypic differences observed between epithelial and mesenchymal cells. Capitalizing on recent advances in cryogenic techniques, volume electron microscopy and super-resolution light microscopy, we studied human mammary epithelial cells in three-dimensional (3D) cultures forming growth-arrested acini. Intriguingly, we found deep nuclear invaginations and tunnels traversing the nucleus, encasing cytoskeletal actin and/or intermediate filaments, which connect to the outer nuclear envelope. The cytoskeleton is also connected both to other cells through desmosome adhesion complexes and to the extracellular matrix through hemidesmosomes. This finding supports a physical and/or mechanical link from the desmosomes and hemidesmosomes to the nucleus, which had previously been hypothesized but now is visualized for the first time. These unique structures, including the nuclear invaginations and the cytoskeletal connectivity to the cell nucleus, are consistent with a dynamic reciprocity between the nucleus and the outside of epithelial cells and tissues.

  19. Field Trial Results of a 14-channel GPR Integrated with a U.S. Program for 3-D Utility Mapping

    NASA Astrophysics Data System (ADS)

    Anspach, James H.

    2013-04-01

    utilities were mostly undetectable. Through a ground-truthing program of test holes to expose utilities, the depth values derived from the enhanced GPR were fairly consistent and within 15 cm of actual depth. The incomplete underground picture determined by the enhanced GPR reinforces previous studies that show that the mapping of existing underground utilities is a multi-tool effort that takes highly trained and skilled field technicians and data interpreters. The addition of a new GPR tool is valuable in determining continuous depth profiles of imaged utilities. A second and significant benefit is the interpretation of other geotechnical data that benefit project designers. This might include showing geometry, location, intensity, and depths of either areas of anomalies, or of known structures, such as paving thickness, substrate thickness, voids, water table, soil lenses, boulders, bedrock, and so forth. The Florida Department of Transportation has decided to take advantage of this new technology and has entered into an experimental contract with Cardno TBE to incorporate several enhanced GPR arrays with traditional utility detection tools. The goal of this contract will be to provide a 3-D model of existing underground utilities for use in automated construction. The GPR 3-D data model will be melded with conventional subsurface utility engineering and mapping practices and will be required to follow the ASCE 38 standard for utility data reliability.

  20. Integration of comprehensive 3D microCT and signaling analysis reveals differential regulatory mechanisms of craniofacial bone development

    PubMed Central

    Ho, Thach-Vu; Iwata, Junichi; Ho, Hoang Anh; Grimes, Weston C.; Park, Shery; Sanchez-Lara, Pedro A.; Chai, Yang

    2015-01-01

    Growth factor signaling regulates tissue-tissue interactions to control organogenesis and tissue homeostasis. Specifically, transforming growth factor beta (TGFβ) signaling plays a crucial role in the development of cranial neural crest (CNC) cell–derived bone, and loss of Tgfbr2 in CNC cells results in craniofacial skeletal malformations. Our recent studies indicate that non-canonical TGFβ signaling is activated whereas canonical TGFβ signaling is compromised in the absence of Tgfbr2 (in Tgfbr2fl/fl;Wnt1-Cre mice). A haploinsufficiency of Tgfbr1 (aka Alk5) (Tgfbr2fl/fl;Wnt1-Cre;Alk5fl/+) largely rescues craniofacial deformities in Tgfbr2 mutant mice by reducing ectopic non-canonical TGFβ signaling. However, the relative involvement of canonical and non-canonical TGFβ signaling in regulating specific craniofacial bone formation remains unclear. We compared the size and volume of CNC–derived craniofacial bones (frontal bone, premaxilla, maxilla, palatine bone, and mandible) from E18.5 control, Tgfbr2fl/fl;Wnt1-Cre, and Tgfbr2fl/fl;Wnt1-Cre;Alk5fl/+ mice. By analyzing three dimensional (3D) micro-computed tomography (microCT) images, we found that different craniofacial bones were restored to different degrees in Tgfbr2fl/fl;Wnt1-Cre;Alk5fl/+ mice. Our study provides comprehensive information on anatomical landmarks and the size and volume of each craniofacial bone, as well as insights into the extent that canonical and non-canonical TGFβ signaling cascades contribute to the formation of each CNC–derived bone. Our data will serve as an important resource for developmental biologists who are interested in craniofacial morphogenesis. PMID:25722190

  1. Trans-Hudsonian far-field deformation effects in the Rae foreland: An integrated geological-3D magnetic model

    NASA Astrophysics Data System (ADS)

    Percival, J. A.; Tschirhart, V.

    2017-03-01

    The intracratonic Rae cover sequence, deposited ca. 2.2-1.9 Ga, forms a useful marker for unravelling tectonic events that affected the Archean Rae Province at ca.2.0, 1.9 and 1.85 Ga. Polyphase deformation is recognized within the Rae cover rocks, including the 70 × 10 km Montresor belt, and attributed to distal effects of the ca. 1.85 Ga Trans-Hudson orogeny. In this contribution we explore the 3D geometry and structural history of the Montresor belt, previously considered to be a simple syncline lying unconformably on Archean basement. New geological, geophysical and geochronological results define a more complex history in which lower Montresor units were thrust-imbricated with basement gneisses and metamorphosed to the amphibolite facies. Mid-to upper greenschist facies upper Montresor units, exposed in an open synform, are superficially less deformed. However, using high-resolution aeromagnetic data and distinct magnetic marker units considered proxies for bedding, we constructed a set of forward models to explore the three-dimensional geometry of the belt. The re-analysis outlines a set of pre-synform structures defined by low-angle truncations of the magnetic markers. Geometric relationships indicate the presence of at least three faults at low angles to bedding, interpreted as D1 piggy-back thrusts, and bracketed by available geochronology between 1.924 and 1.87 Ga. D1 strain in the upper Montresor strata is significantly less intense than that further south in Rae cover rocks, consistent with a more distal foreland setting during the Trans-Hudson orogeny. The Montresor belt preserves a record of the Trans-Hudson tectonic style at relatively shallow crustal levels as a result of its foreland setting and structural history including a syn-orogenic extensional detachment event.

  2. High-efficiency and wideband interlayer grating couplers in multilayer Si/SiO2/SiN platform for 3D integration of optical functionalities.

    PubMed

    Sodagar, Majid; Pourabolghasem, Reza; Eftekhar, Ali A; Adibi, Ali

    2014-07-14

    We have designed interlayer grating couplers with single/double metallic reflectors for Si/SiO(2)/SiN multilayer material platform. Out-of-plane diffractive grating couplers separated by 1.6 μm thick buffer SiO(2) layer are vertically stacked against each other in Si and SiN layers. Geometrical optimization using genetic algorithm coupled with electromagnetic simulations using two-dimensional (2D) finite element method (FEM) results in coupler designs with high peak coupling efficiency of up to 89% for double- mirror and 64% for single-mirror structures at telecom wavelength. Also, 3-dB bandwidths of 40 nm and 50 nm are theoretically predicted for the two designs, respectively. We have fabricated the grating coupler structure with single mirror. Measured values for insertion loss and 3-dB bandwidth in the fabricated single-mirror coupler confirms the theoretical results. This opens up the possibility of low-loss 3D dense integration of optical functionalities in hybrid material platforms.

  3. 3D vision system assessment

    NASA Astrophysics Data System (ADS)

    Pezzaniti, J. Larry; Edmondson, Richard; Vaden, Justin; Hyatt, Bryan; Chenault, David B.; Kingston, David; Geulen, Vanilynmae; Newell, Scott; Pettijohn, Brad

    2009-02-01

    In this paper, we report on the development of a 3D vision system consisting of a flat panel stereoscopic display and auto-converging stereo camera and an assessment of the system's use for robotic driving, manipulation, and surveillance operations. The 3D vision system was integrated onto a Talon Robot and Operator Control Unit (OCU) such that direct comparisons of the performance of a number of test subjects using 2D and 3D vision systems were possible. A number of representative scenarios were developed to determine which tasks benefited most from the added depth perception and to understand when the 3D vision system hindered understanding of the scene. Two tests were conducted at Fort Leonard Wood, MO with noncommissioned officers ranked Staff Sergeant and Sergeant First Class. The scenarios; the test planning, approach and protocols; the data analysis; and the resulting performance assessment of the 3D vision system are reported.

  4. Successful integration of 3-D seismic and multidisciplinary approaches in exploring the Zechstein 2 carbonates in northeast Netherlands

    SciTech Connect

    Casson, N. ); Van Wees, B.; Reijers, T. ); Henk Rebel )

    1993-09-01

    Nederlandse Aardolie Maatschappij has been actively involved in the exploration of the Zechstein 2 carbonate play for over 40 yr. This exploration effort, concentrated mainly in the Drenthe and Schoonebeek area of northeast Netherlands, has resulted in the discovery of 20 gas fields with cumulative reserves of more than 40 x 10[sup 9] m[sup 3] of gas. The wealth of subsurface data collected in the exploration and development of these fields has lead to the establishment of detailed depositional and diagenetic models of the Zechstein 2 carbonates. More recently, extensive three-dimensional seismic coverage, the integration of lateral prediction techniques, and detailed fracture studies in cores have provided powerful predicting tools in the comprehension and exploitation of the play. Successful integration of the various disciplines now allows exploration of subtle low-relief structures in the mature Zechstein hydrocarbon province and helps target directional exploration and development wells.

  5. Preconditioning techniques for constrained vector potential integral equations, with application to 3-D magnetoquasistatic analysis of electronic packages

    SciTech Connect

    Kamon, M.; Phillips, J.R.

    1994-12-31

    In this paper techniques are presented for preconditioning equations generated by discretizing constrained vector integral equations associated with magnetoquasistatic analysis. Standard preconditioning approaches often fail on these problems. The authors present a specialized preconditioning technique and prove convergence bounds independent of the constraint equations and electromagnetic excitation frequency. Computational results from analyzing several electronic packaging examples are given to demonstrate that the new preconditioning approach can sometimes reduce the number of GMRES iterations by more than an order of magnitude.

  6. A 3D Human-Machine Integrated Design and Analysis Framework for Squat Exercises with a Smith Machine

    PubMed Central

    Lee, Haerin; Jung, Moonki; Lee, Ki-Kwang; Lee, Sang Hun

    2017-01-01

    In this paper, we propose a three-dimensional design and evaluation framework and process based on a probabilistic-based motion synthesis algorithm and biomechanical analysis system for the design of the Smith machine and squat training programs. Moreover, we implemented a prototype system to validate the proposed framework. The framework consists of an integrated human–machine–environment model as well as a squat motion synthesis system and biomechanical analysis system. In the design and evaluation process, we created an integrated model in which interactions between a human body and machine or the ground are modeled as joints with constraints at contact points. Next, we generated Smith squat motion using the motion synthesis program based on a Gaussian process regression algorithm with a set of given values for independent variables. Then, using the biomechanical analysis system, we simulated joint moments and muscle activities from the input of the integrated model and squat motion. We validated the model and algorithm through physical experiments measuring the electromyography (EMG) signals, ground forces, and squat motions as well as through a biomechanical simulation of muscle forces. The proposed approach enables the incorporation of biomechanics in the design process and reduces the need for physical experiments and prototypes in the development of training programs and new Smith machines. PMID:28178184

  7. A 3D Human-Machine Integrated Design and Analysis Framework for Squat Exercises with a Smith Machine.

    PubMed

    Lee, Haerin; Jung, Moonki; Lee, Ki-Kwang; Lee, Sang Hun

    2017-02-06

    In this paper, we propose a three-dimensional design and evaluation framework and process based on a probabilistic-based motion synthesis algorithm and biomechanical analysis system for the design of the Smith machine and squat training programs. Moreover, we implemented a prototype system to validate the proposed framework. The framework consists of an integrated human-machine-environment model as well as a squat motion synthesis system and biomechanical analysis system. In the design and evaluation process, we created an integrated model in which interactions between a human body and machine or the ground are modeled as joints with constraints at contact points. Next, we generated Smith squat motion using the motion synthesis program based on a Gaussian process regression algorithm with a set of given values for independent variables. Then, using the biomechanical analysis system, we simulated joint moments and muscle activities from the input of the integrated model and squat motion. We validated the model and algorithm through physical experiments measuring the electromyography (EMG) signals, ground forces, and squat motions as well as through a biomechanical simulation of muscle forces. The proposed approach enables the incorporation of biomechanics in the design process and reduces the need for physical experiments and prototypes in the development of training programs and new Smith machines.

  8. Increment of Access Points in Integrated System of Wavelength Division Multiplexed Passive Optical Network Radio over Fiber.

    PubMed

    Amiri, I S; Alavi, S E; Soltanian, M R K; Fisal, N; Supa'at, A S M; Ahmad, H

    2015-07-08

    This paper describes a novel technique to increase the numbers of access points (APs) in a wavelength division multiplexed-passive optical network (WDM-PON) integrated in a 100 GHz radio-over-fiber (RoF). Eight multi-carriers separated by 25 GHz intervals were generated in the range of 193.025 to 193.200 THz using a microring resonator (MRR) system incorporating an add-drop filter system. All optically generated multi-carriers were utilized in an integrated system of WDM-PON-RoF for transmission of four 43.6 Gb/sec orthogonal frequency division multiplexing (OFDM) signals. Results showed that an acceptable BER variation for different path lengths up to 25 km was achievable for all four access points and thus the transmission of four OFDM channels is feasible for a 25 km standard single mode fiber (SSMF) path length.

  9. Fully integrated multiplexed lab-on-a-card assay for enteric pathogens

    NASA Astrophysics Data System (ADS)

    Weigl, B. H.; Gerdes, J.; Tarr, P.; Yager, P.; Dillman, L.; Peck, R.; Ramachandran, S.; Lemba, M.; Kokoris, M.; Nabavi, M.; Battrell, F.; Hoekstra, D.; Klein, E. J.; Denno, D. M.

    2006-01-01

    Under this NIH-funded project, we are developing a lab-on-a-card platform to identify enteric bacterial pathogens in patients presenting with acute diarrhea, with special reference to infections that might be encountered in developing countries. Component functions that are integrated on this platform include on-chip immunocapture of live or whole pathogens, multiplexed nucleic acid amplification and on-chip detection, sample processing to support direct use of clinical specimens, and dry reagent storage and handling. All microfluidic functions are contained on the lab card. This new diagnostic test will be able to rapidly identify and differentiate Shigella dysenteriae serotype 1, Shigella toxin-producing Escherichia coli, E. coli 0157, Campylobacter jejuni, and Salmonella and Shigella species. This presentation will report on progress to date on sample and bacteria processing methodologies, identification and validation of capture antibodies and strategy for organism immunocapture, identification and validation of specific polymerase chain reaction (PCR) primer sequences for over 200 clinical isolates of enteric pathogens, and implementation of on-chip nucleic acid extraction for a subset of those pathogens.

  10. Design of H-PDLC grating array chopper applied in frequency division multiplexed integrated confocal microscopy

    NASA Astrophysics Data System (ADS)

    Wen, Ken; Zheng, Jihong; Sun, Guoqiang; Liu, Defeng; Chen, Luoyang; Zhuang, Songlin

    2009-11-01

    A new type of electrically controlled optical chopper based on Holographic Polymer Dispersed Liquid Crystal (HPDLC) grating array is demonstrated in this paper. H-PDLC grating can be erased and become transparent to the incident beam when applied the external voltage. H-PDLC grating recovers and diffracts incident light in the original way after removal the driving voltage. Thus the intensity and the frequency modulation to the incident beam can be achieved through adjusting the voltage applied on the H-PDLC cell. Compared with a conventional chopper, the H-PDLC gratings exhibit more advantage like much faster response time, the grating array is much more integrated and easier to be fabricated etc. Moreover, electro-optical properties including response time and diffraction efficiency of H-PDLC gratings are investigated. By optimizing some parameters, H-PDLC chopper cell with a microsecond response time and high diffraction efficiency is obtained. The driving voltage of H-PDLC can be reduced through controlling the droplet size in H-PDLC and the thickness of the grating. As the application example, the chopper is applied in the new born frequency division multiplexed multichannel fluorescence confocal microscopy so that it can improve the temporal and spatial resolution ability of the microscopy system.

  11. Integration of microfluidic chip with biomimetic hydrogel for 3D controlling and monitoring of cell alignment and migration.

    PubMed

    Lee, Kwang Ho; Lee, Ki Hwa; Lee, Jeonghoon; Choi, Hyuk; Lee, Donghee; Park, Yongdoo; Lee, Sang-Hoon

    2014-04-01

    A biomimetic hydrogel was integrated into microfluidic chips to monitor glioma cell alignment and migration. The extracellular matrix-based biomimetic hydrogel was remodeled by matrix metalloprotease (MMP) secreted by glioma cells and the hydrogel could thus be used to assess cellular behavior. Both static and dynamic cell growth conditions (flow rate of 0.1 mL/h) were used. Cell culture medium with and without vascular endothelial growth factor (VEGF), insensitive VEGF and tissue inhibitor of metalloproteinases (TIMP) were employed to monitor cell behavior. A concentration gradient formed in the hydrogel resulted in differences in cell behavior. Glioma cell viability in the microchannel was 75-85%. Cells in the VEGF-loaded microchannels spread extensively, degrading the MMP-sensitive hydrogel, and achieved cell sizes almost fivefold larger than seen in the control medium. Our integrated system can be used as a model for the study of cellular behavior in a controlled microenvironment generated by fluidic conditions in a biomimetic matrix.

  12. Study on characteristics of 3-D translating-pulsating source green function of deep-water havelock form and its fast integration method

    NASA Astrophysics Data System (ADS)

    Xu, Yong; Dong, Wen-Cai

    2011-09-01

    The singularities, oscillatory performances and the contributing factors to the 3-D translating-pulsating source Green function of deep-water Havelock form which consists of a local disturbance part and a far-field wave-like part, are analyzed systematically. Relative numerical integral methods about the two parts are presented in this paper. An improved method based on LOBATTO rule is used to eliminate singularities caused respectively by infinite discontinuity and jump discontinuous node from the local disturbance part function, which makes the improvement of calculation efficiency and accuracy possible. And variable substitution is applied to remove the singularity existing at the end of the integral interval of the far-field wave-like part function. Two auxiliary techniques such as valid interval calculation and local refinement of integral steps technique in narrow zones near false singularities are applied so as to avoid unnecessary integration of invalid interval and improve integral accordance. Numerical test results have proved the efficiency and accuracy in these integral methods that thus can be applied to calculate hydrodynamic performance of floating structures moving in waves.

  13. Data Integration Acquired from Micro-Uav and Terrestrial Laser Scanner for the 3d Mapping of Jesuit Ruins of São Miguel das Missões

    NASA Astrophysics Data System (ADS)

    Reiss, M. L. L.; da Rocha, R. S.; Ferraz, R. S.; Cruz, V. C.; Morador, L. Q.; Yamawaki, M. K.; Rodrigues, E. L. S.; Cole, J. O.; Mezzomo, W.

    2016-06-01

    The Jesuit Missions the Guaranis were one of the great examples of cultural, social, and scientific of the eighteenth century, which had its decline from successive wars that followed the exchange of territories domain occupied by Portugal and Spain with the Madrid Treaty of January 13, 1750. One of the great examples of this development is materialized in the ruins of 30 churches and villages that remain in a territory that now comprises part of Brazil, Argentina and Paraguay. These Churches, São Miguel das Missões is among the Brazilian ruins, the best preserved. The ruins of São Miguel das Missões were declared a UNESCO World Cultural Heritage in 1983 and the Institute of National Historical Heritage (IPHAN) is the Brazilian Federal agency that manages and maintains this heritage. In order to produce a geographic database to assist the IPHAN in the management of the Ruins of São Miguel das Missões it was proposed a three-dimensional mapping of these ruins never performed in this location before. The proposal is integrated data acquired from multiple sensors: two micro-UAV, an Asctec Falcon 8 (rotary wing) and a Sensefly e-Bee (fixed wing); photos from terrestrial cameras; two terrestrial LIDAR sensors, one Faro Focus 3D S-120 and Optec 3D-HD ILRIS. With this abundance of sensors has been possible to perform comparisons and integration of the acquired data, and produce a 3D reconstruction of the church with high completeness and accuracy (better than 25 mm), as can be seen in the presentation of this work.

  14. Reality and Surreality of 3-D Displays: Holodeck and Beyond

    DTIC Science & Technology

    2000-01-01

    Holodeck is the reality that significantly better 3D display systems are possible. Keywords: true 3D displays, multiplexed 2D display ( autostereoscopic ...displays still do not use them in their own offices. Thus, 3D approaches that are autostereoscopic (that is, no-head gear is required) are preferred. A...challenges noted throughout the aforegoing sections of this paper will be steadily overcome. True 3D , autostereoscopic (no head gear) monitors with usable

  15. Automatic 3D Building Model Generation by Integrating LiDAR and Aerial Images Using a Hybrid Approach

    NASA Astrophysics Data System (ADS)

    Kwak, Eunju

    The development of sensor technologies and the increase in user requirements have resulted in many different approaches for efficient building model generation. Three-dimensional building models are important in various applications, such as disaster management and urban planning. Despite this importance, generation of these models lacks economical and reliable techniques which take advantage of the available multi-sensory data from single and multiple platforms. Therefore, this research develops a framework for fully-automated building model generation by integrating data-driven and model-driven methods as well as exploiting the advantages of images and LiDAR datasets. The building model generation starts by employing LiDAR data for building detection and approximate boundary determination. The generated building boundaries are then integrated into a model-based image processing strategy, because LiDAR derived planes show irregular boundaries due to the nature of LiDAR point acquisition. The focus of the research is generating models for the buildings with right-angled-corners, which can be described with a collection of rectangles (e.g., L-shape, T-shape, U-shape, gable roofs, and more complex building shapes which are combinations of the aforementioned shapes), under the assumption that the majority of the buildings in urban areas belong to this category. Therefore, by applying the Minimum Bounding Rectangle (MBR) algorithm recursively, the LiDAR boundaries are decomposed into sets of rectangles for further processing. At the same time the quality of the MBRs are examined to verify that the buildings, from which the boundaries are generated, are buildings with right-angled-corners. These rectangles are preliminary model primitives. The parameters that define the model primitives are adjusted using detected edges in the imagery through the least-squares adjustment procedure, i.e., model-based image fitting. The level of detail in the final Digital Building Model

  16. STING Millennium Suite: integrated software for extensive analyses of 3d structures of proteins and their complexes

    PubMed Central

    Higa, Roberto H; Togawa, Roberto C; Montagner, Arnaldo J; Palandrani, Juliana CF; Okimoto, Igor KS; Kuser, Paula R; Yamagishi, Michel EB; Mancini, Adauto L; Neshich, Goran

    2004-01-01

    Background The integration of many aspects of protein/DNA structure analysis is an important requirement for software products in general area of structural bioinformatics. In fact, there are too few software packages on the internet which can be described as successful in this respect. We might say that what is still missing is publicly available, web based software for interactive analysis of the sequence/structure/function of proteins and their complexes with DNA and ligands. Some of existing software packages do have certain level of integration and do offer analysis of several structure related parameters, however not to the extent generally demanded by a user. Results We are reporting here about new Sting Millennium Suite (SMS) version which is fully accessible (including for local files at client end), web based software for molecular structure and sequence/structure/function analysis. The new SMS client version is now operational also on Linux boxes and it works with non-public pdb formatted files (structures not deposited at the RCSB/PDB), eliminating earlier requirement for the registration if SMS components were to be used with user's local files. At the same time the new SMS offers some important additions and improvements such as link to ProTherm as well as significant re-engineering of SMS component ConSSeq. Also, we have added 3 new SMS mirror sites to existing network of global SMS servers: Argentina, Japan and Spain. Conclusion SMS is already established software package and many key data base and software servers worldwide, do offer either a link to, or host the SMS. SMS (Sting Millennium Suite) is web-based publicly available software developed to aid researches in their quest for translating information about the structures of macromolecules into knowledge. SMS allows to a user to interactively analyze molecular structures, cross-referencing visualized information with a correlated one, available across the internet. SMS is already used as a

  17. Ultralow-loss polycrystalline silicon waveguides and high uniformity 1x12 MMI fanout for 3D photonic integration.

    PubMed

    Kwong, David; Covey, John; Hosseini, Amir; Zhang, Yang; Xu, Xiaochuan; Chen, Ray T

    2012-09-10

    We have investigated the feasibility of multimode polysilicon waveguides to demonstrate the suitability of polysilicon as a candidate for multilayer photonic applications. Solid Phase Crystallization (SPC) with a maximum temperature of 1000°C is used to create polysilicon on thermally grown SiO2. We then measure the propagation losses for various waveguide widths on both polysilicon and crystalline silicon platforms. We find that as the width increases for polysilicon waveguides, the propagation loss decreases similar to crystalline silicon waveguides. At a waveguide width of 10 µm, polysilicon and crystalline silicon waveguides have propagation losses of 0.56 dB/cm and 0.31 dB/cm, respectively, indicating there is little bulk absorption from the polysilicon and is the lowest propagation loss for polysilicon demonstrated to date. In addition, the first 1x12 polysilicon MMI is demonstrated with a low insertion loss of -1.29dB and a high uniformity of 1.07 dB. These results vindicate the use of polysilicon waveguides of varying widths in photonic integrated circuits.

  18. Three-dimensional (3D) coseismic deformation map produced by the 2014 South Napa Earthquake estimated and modeled by SAR and GPS data integration

    NASA Astrophysics Data System (ADS)

    Polcari, Marco; Albano, Matteo; Fernández, José; Palano, Mimmo; Samsonov, Sergey; Stramondo, Salvatore; Zerbini, Susanna

    2016-04-01

    In this work we present a 3D map of coseismic displacements due to the 2014 Mw 6.0 South Napa earthquake, California, obtained by integrating displacement information data from SAR Interferometry (InSAR), Multiple Aperture Interferometry (MAI), Pixel Offset Tracking (POT) and GPS data acquired by both permanent stations and campaigns sites. This seismic event produced significant surface deformation along the 3D components causing several damages to vineyards, roads and houses. The remote sensing results, i.e. InSAR, MAI and POT, were obtained from the pair of SAR images provided by the Sentinel-1 satellite, launched on April 3rd, 2014. They were acquired on August 7th and 31st along descending orbits with an incidence angle of about 23°. The GPS dataset includes measurements from 32 stations belonging to the Bay Area Regional Deformation Network (BARDN), 301 continuous stations available from the UNAVCO and the CDDIS archives, and 13 additional campaign sites from Barnhart et al, 2014 [1]. These data constrain the horizontal and vertical displacement components proving to be helpful for the adopted integration method. We exploit the Bayes theory to search for the 3D coseismic displacement components. In particular, for each point, we construct an energy function and solve the problem to find a global minimum. Experimental results are consistent with a strike-slip fault mechanism with an approximately NW-SE fault plane. Indeed, the 3D displacement map shows a strong North-South (NS) component, peaking at about 15 cm, a few kilometers far from the epicenter. The East-West (EW) displacement component reaches its maximum (~10 cm) south of the city of Napa, whereas the vertical one (UP) is smaller, although a subsidence in the order of 8 cm on the east side of the fault can be observed. A source modelling was performed by inverting the estimated displacement components. The best fitting model is given by a ~N330° E-oriented and ~70° dipping fault with a prevailing

  19. Euro3D Science Conference

    NASA Astrophysics Data System (ADS)

    Walsh, J. R.

    2004-02-01

    The Euro3D RTN is an EU funded Research Training Network to foster the exploitation of 3D spectroscopy in Europe. 3D spectroscopy is a general term for spectroscopy of an area of the sky and derives its name from its two spatial + one spectral dimensions. There are an increasing number of instruments which use integral field devices to achieve spectroscopy of an area of the sky, either using lens arrays, optical fibres or image slicers, to pack spectra of multiple pixels on the sky (``spaxels'') onto a 2D detector. On account of the large volume of data and the special methods required to reduce and analyse 3D data, there are only a few centres of expertise and these are mostly involved with instrument developments. There is a perceived lack of expertise in 3D spectroscopy spread though the astronomical community and its use in the armoury of the observational astronomer is viewed as being highly specialised. For precisely this reason the Euro3D RTN was proposed to train young researchers in this area and develop user tools to widen the experience with this particular type of data in Europe. The Euro3D RTN is coordinated by Martin M. Roth (Astrophysikalisches Institut Potsdam) and has been running since July 2002. The first Euro3D science conference was held in Cambridge, UK from 22 to 23 May 2003. The main emphasis of the conference was, in keeping with the RTN, to expose the work of the young post-docs who are funded by the RTN. In addition the team members from the eleven European institutes involved in Euro3D also presented instrumental and observational developments. The conference was organized by Andy Bunker and held at the Institute of Astronomy. There were over thirty participants and 26 talks covered the whole range of application of 3D techniques. The science ranged from Galactic planetary nebulae and globular clusters to kinematics of nearby galaxies out to objects at high redshift. Several talks were devoted to reporting recent observations with newly

  20. An efficient formulation of the coupled finite element-integral equation technique for solving large 3D scattering problems

    NASA Technical Reports Server (NTRS)

    Cwik, T.; Jamnejad, V.; Zuffada, C.

    1993-01-01

    It is often desirable to calculate the electromagnetic fields inside and about a complicated system of scattering bodies, as well as in their far-field region. The finite element method (FE) is well suited to solving the interior problem, but the domain has to be limited to a manageable size. At the truncation of the FE mesh one can either impose approximate (absorbing) boundary conditions or set up an integral equation (IE) for the fields scattered from the bodies. The latter approach is preferable since it results in higher accuracy. Hence, the two techniques can be successfully combined by introducing a surface that encloses the scatterers, applying a FE model to the inner volume and setting up an IE for the tangential fields components on the surface. Here the continuity of the tangential fields is used bo obtain a consistent solution. A few coupled FE-IE methods have recently appeared in the literature. The approach presented here has the advantage of using edge-based finite elements, a type of finite elements with degrees of freedom associated with edges of the mesh. Because of their properties, they are better suited than the conventional node based elements to represent electromagnetic fields, particularly when inhomogeneous regions are modeled, since the node based elements impose an unnatural continuity of all field components across boundaries of mesh elements. Additionally, our approach is well suited to handle large size problems and lends itself to code parallelization. We will discuss the salient features that make our approach very efficient from the standpoint of numerical computation, and the fields and RCS of a few objects are illustrated as examples.

  1. Integration of a Decentralized Linear-Quadratic-Gaussian Control into GSFC's Universal 3-D Autonomous Formation Flying Algorithm

    NASA Technical Reports Server (NTRS)

    Folta, David C.; Carpenter, J. Russell

    1999-01-01

    A decentralized control is investigated for applicability to the autonomous formation flying control algorithm developed by GSFC for the New Millenium Program Earth Observer-1 (EO-1) mission. This decentralized framework has the following characteristics: The approach is non-hierarchical, and coordination by a central supervisor is not required; Detected failures degrade the system performance gracefully; Each node in the decentralized network processes only its own measurement data, in parallel with the other nodes; Although the total computational burden over the entire network is greater than it would be for a single, centralized controller, fewer computations are required locally at each node; Requirements for data transmission between nodes are limited to only the dimension of the control vector, at the cost of maintaining a local additional data vector. The data vector compresses all past measurement history from all the nodes into a single vector of the dimension of the state; and The approach is optimal with respect to standard cost functions. The current approach is valid for linear time-invariant systems only. Similar to the GSFC formation flying algorithm, the extension to linear LQG time-varying systems requires that each node propagate its filter covariance forward (navigation) and controller Riccati matrix backward (guidance) at each time step. Extension of the GSFC algorithm to non-linear systems can also be accomplished via linearization about a reference trajectory in the standard fashion, or linearization about the current state estimate as with the extended Kalman filter. To investigate the feasibility of the decentralized integration with the GSFC algorithm, an existing centralized LQG design for a single spacecraft orbit control problem is adapted to the decentralized framework while using the GSFC algorithm's state transition matrices and framework. The existing GSFC design uses both reference trajectories of each spacecraft in formation and

  2. A heterogeneous sensor network simulation system with integrated terrain data for real-time target detection in 3D space

    NASA Astrophysics Data System (ADS)

    Lin, Hong; Tanner, Steve; Rushing, John; Graves, Sara; Criswell, Evans

    2008-03-01

    Large scale sensor networks composed of many low-cost small sensors networked together with a small number of high fidelity position sensors can provide a robust, fast and accurate air defense and warning system. The team has been developing simulations of such large networks, and is now adding terrain data in an effort to provide more realistic analysis of the approach. This work, a heterogeneous sensor network simulation system with integrated terrain data for real-time target detection in a three-dimensional environment is presented. The sensor network can be composed of large numbers of low fidelity binary and bearing-only sensors, and small numbers of high fidelity position sensors, such as radars. The binary and bearing-only sensors are randomly distributed over a large geographic region; while the position sensors are distributed evenly. The elevations of the sensors are determined through the use of DTED Level 0 dataset. The targets are located through fusing measurement information from all types of sensors modeled by the simulation. The network simulation utilizes the same search-based optimization algorithm as in our previous two-dimensional sensor network simulation with some significant modifications. The fusion algorithm is parallelized using spatial decomposition approach: the entire surveillance area is divided into small regions and each region is assigned to one compute node. Each node processes sensor measurements and terrain data only for the assigned sub region. A master process combines the information from all the compute nodes to get the overall network state. The simulation results have indicated that the distributed fusion algorithm is efficient enough so that an optimal solution can be reached before the arrival of the next sensor data with a reasonable time interval, and real-time target detection can be achieved. The simulation was performed on a Linux cluster with communication between nodes facilitated by the Message Passing Interface

  3. Numerical modeling of the 3D dynamics of ultrasound contrast agent microbubbles using the boundary integral method

    NASA Astrophysics Data System (ADS)

    Wang, Qianxi; Manmi, Kawa; Calvisi, Michael L.

    2015-02-01

    Ultrasound contrast agents (UCAs) are microbubbles stabilized with a shell typically of lipid, polymer, or protein and are emerging as a unique tool for noninvasive therapies ranging from gene delivery to tumor ablation. While various models have been developed to describe the spherical oscillations of contrast agents, the treatment of nonspherical behavior has received less attention. However, the nonspherical dynamics of contrast agents are thought to play an important role in therapeutic applications, for example, enhancing the uptake of therapeutic agents across cell membranes and tissue interfaces, and causing tissue ablation. In this paper, a model for nonspherical contrast agent dynamics based on the boundary integral method is described. The effects of the encapsulating shell are approximated by adapting Hoff's model for thin-shell, spherical contrast agents. A high-quality mesh of the bubble surface is maintained by implementing a hybrid approach of the Lagrangian method and elastic mesh technique. The numerical model agrees well with a modified Rayleigh-Plesset equation for encapsulated spherical bubbles. Numerical analyses of the dynamics of UCAs in an infinite liquid and near a rigid wall are performed in parameter regimes of clinical relevance. The oscillation amplitude and period decrease significantly due to the coating. A bubble jet forms when the amplitude of ultrasound is sufficiently large, as occurs for bubbles without a coating; however, the threshold amplitude required to incite jetting increases due to the coating. When a UCA is near a rigid boundary subject to acoustic forcing, the jet is directed towards the wall if the acoustic wave propagates perpendicular to the boundary. When the acoustic wave propagates parallel to the rigid boundary, the jet direction has components both along the wave direction and towards the boundary that depend mainly on the dimensionless standoff distance of the bubble from the boundary. In all cases, the jet

  4. The GLOBE 3D Genome Platform - towards a novel system-biological paper tool to integrate the huge complexity of genome organization and function.

    PubMed

    Knoch, Tobias A; Lesnussa, Michael; Kepper, Nick; Eussen, Hubert B; Grosveld, Frank G

    2009-01-01

    Genomes are tremendous co-evolutionary holistic systems for molecular storage, processing and fabrication of information. Their system-biological complexity remains, however, still largely mysterious, despite immense sequencing achievements and huge advances in the understanding of the general sequential, three-dimensional and regulatory organization. Here, we present the GLOBE 3D Genome Platform a completely novel grid based virtual "paper" tool and in fact the first system-biological genome browser integrating the holistic complexity of genomes in a single easy comprehensible platform: Based on a detailed study of biophysical and IT requirements, every architectural level from sequence to morphology of one or several genomes can be approached in a real and in a symbolic representation simultaneously and navigated by continuous scale-free zooming within a unique three-dimensional OpenGL and grid driven environment. In principle an unlimited number of multi-dimensional data sets can be visualized, customized in terms of arrangement, shape, colour, and texture etc. as well as accessed and annotated individually or in groups using internal or external data bases/facilities. Any information can be searched and correlated by importing or calculating simple relations in real-time using grid resources. A general correlation and application platform for more complex correlative analysis and a front-end for system-biological simulations both using again the huge capabilities of grid infrastructures is currently under development. Hence, the GLOBE 3D Genome Platform is an example of a grid based approach towards a virtual desktop for genomic work combining the three fundamental distributed resources: i) visual data representation, ii) data access and management, and iii) data analysis and creation. Thus, the GLOBE 3D Genome Platform is the novel system-biology oriented information system urgently needed to access, present, annotate, and to simulate the holistic genome

  5. Design, in vitro and in vivo assessment of a multi-channel sieve electrode with integrated multiplexer.

    PubMed

    Ramachandran, Anup; Schuettler, Martin; Lago, Natalia; Doerge, Thomas; Koch, Klaus Peter; Navarro, Xavier; Hoffmann, Klaus-Peter; Stieglitz, Thomas

    2006-06-01

    This paper reports on the design, in vitro and in vivo investigation of a flexible, lightweight, polyimide based implantable sieve electrode with a hybrid assembly of multiplexers and polymer encapsulation. The integration of multiplexers enables us to connect a large number of electrodes on the sieve using few input connections. The implant assembly of the sieve electrode with the electronic circuitry was verified by impedance measurement. The 27 platinum electrodes of the sieve were coated with platinum black to reduce the electrode impedance. The impedance magnitude of the electrode sites on the sieve (geometric surface area 2,200 microm(2)) was |Z(f=1kHz)| = 5.7 kOmega. The sieve electrodes, encased in silicone, have been implanted in the transected sciatic nerve of rats. Initial experiments showed that axons regenerated through the holes of the sieve and reinnervated distal target organs. Nerve signals were recorded in preliminary tests after 3-7 months post-implantation.

  6. The integration of 3D electrical resistivity tomography and ET flux measurements to characterize water mass balance in the soil-plant-atmosphere continuum

    NASA Astrophysics Data System (ADS)

    Vanella, Daniela; Boaga, Jacopo; Perri, Maria Teresa; Consoli, Simona; Cassiani, Giorgio

    2014-05-01

    The system of soil, vegetation, and the adjacent atmosphere is characterized by complex patterns, structures, and processes that act on a wide range of time and space scales. While the exchange of energy and water is continuous between compartments, the pertinent fluxes are strongly heterogeneous and variable in space and time. Therefore, quantitatively predicting the systems' behaviour constitutes a major challenge. Traditionally, soil moisture beneath irrigated crops has been determined using point measurement methods such as neutron probes or capacitance systems. These approaches cannot measure soil moisture at depths beyond the root-zone of plants and have limited lateral coverage. Literature results show that electrical resistivity tomography (ERT) can be used to reliable map the spatial heterogeneity in soil moisture. Here we present the application of the time-lapse non-invasive 3D micro - electrical tomography (ERT) to monitor soil-plant interactions in the root zone of an orange tree located in the Mediterranean semi-arid Sicilian (South Italy) context. The subsoil dynamics, particularly influenced by irrigation and root uptake, has been characterized a 3D ERT apparatus consisting of 48 buried electrodes on 4 instrumented micro boreholes plus 24 mini-electrodes on the surface spaced 0.1 m on a square grid. During the monitoring, repeated ERT soil moisture measurements were collected, as well as laboratory characterization of the soil electrical properties as a function of moisture content and pore water electrical conductivity. Plant transpiration was continuously monitored during the ERT experiment by the sap flow heat pulse (HP) method for a quantitative analysis of the mass balance in the soil-plant-atmosphere system under observation. In addition, evapo-transpiration has been continuously monitored at the same site using an eddy-correlation tower. The integration of measurements regarding soil,plant and atmosphere allows a better understanding of

  7. Integrated methodologies for the 3d survey and the structural monitoring of industrial archaeology: the case of the Casalecchio di Reno Dyke, Italy

    NASA Astrophysics Data System (ADS)

    Bitelli, Gabriele; Girelli, Valentina A.; Vittuari, Luca; Zanutta, Antonio

    2010-05-01

    The concept of "Industrial Archaeology" was introduced in the 1950s in England in order to consider the branch of Archaeology that, in integration with engineering, architecture and economics, studies the industrial past, with the purpose of document, protect and exploite the industrial-cultural heritage. The systematic study of this kind of structures and artefacts need a wide range approach, that starts from the investigation about the object historic fonts until its complete qualitative and quantitative description, also considering the characteristics of the surrounding territory. This task often needs the integration of different survey techniques for data capturing and processing, in order to perform the 3D modeling and to analize the structural deformations of manufactured articles. The paper presents the surveying and monitoring activities on the important structure of the Casalecchio di Reno dyke, near Bologna. The first historic notes on the dyke are very old; they are dated back to the I millenium A. D. and report the news of a wooden barrage of the Reno canal. The construction of the actual structure is in the period 1360-1367 and suffered during the centuries numerous interventions and restores. The surveying activities on the dyke were realized in 2005-2006 and 2009 and involved different techniques (classical topography, high precision geometric levelling, range-based method by terrestrial laser scanning, digital photogrammetry, thermal imagery) integrated together in a unique local reference system, in order to study the stability and the movements of the structure in a established period of time and to realize a 3D model. Together with detail surveys on the single parts of the structure, an analysis at a larger territorial scale was performed by aerial photogrammetry, either using current imagery and historical data. The topographic measurements with traditional differential techniques have achieved a very high level precision and the realized

  8. 3d-3d correspondence revisited

    DOE PAGES

    Chung, Hee -Joong; Dimofte, Tudor; Gukov, Sergei; ...

    2016-04-21

    In fivebrane compactifications on 3-manifolds, we point out the importance of all flat connections in the proper definition of the effective 3d N = 2 theory. The Lagrangians of some theories with the desired properties can be constructed with the help of homological knot invariants that categorify colored Jones polynomials. Higgsing the full 3d theories constructed this way recovers theories found previously by Dimofte-Gaiotto-Gukov. As a result, we also consider the cutting and gluing of 3-manifolds along smooth boundaries and the role played by all flat connections in this operation.

  9. Identifying Structure-Property Relationships Through DREAM.3D Representative Volume Elements and DAMASK Crystal Plasticity Simulations: An Integrated Computational Materials Engineering Approach

    NASA Astrophysics Data System (ADS)

    Diehl, Martin; Groeber, Michael; Haase, Christian; Molodov, Dmitri A.; Roters, Franz; Raabe, Dierk

    2017-03-01

    Predicting, understanding, and controlling the mechanical behavior is the most important task when designing structural materials. Modern alloy systems—in which multiple deformation mechanisms, phases, and defects are introduced to overcome the inverse strength-ductility relationship—give raise to multiple possibilities for modifying the deformation behavior, rendering traditional, exclusively experimentally-based alloy development workflows inappropriate. For fast and efficient alloy design, it is therefore desirable to predict the mechanical performance of candidate alloys by simulation studies to replace time- and resource-consuming mechanical tests. Simulation tools suitable for this task need to correctly predict the mechanical behavior in dependence of alloy composition, microstructure, texture, phase fractions, and processing history. Here, an integrated computational materials engineering approach based on the open source software packages DREAM.3D and DAMASK (Düsseldorf Advanced Materials Simulation Kit) that enables such virtual material development is presented. More specific, our approach consists of the following three steps: (1) acquire statistical quantities that describe a microstructure, (2) build a representative volume element based on these quantities employing DREAM.3D, and (3) evaluate the representative volume using a predictive crystal plasticity material model provided by DAMASK. Exemplarily, these steps are here conducted for a high-manganese steel.

  10. Integrating 3D Flower-Like Hierarchical Cu2NiSnS4 with Reduced Graphene Oxide as Advanced Anode Materials for Na-Ion Batteries.

    PubMed

    Yuan, Shuang; Wang, Sai; Li, Lin; Zhu, Yun-hai; Zhang, Xin-bo; Yan, Jun-min

    2016-04-13

    Development of an anode material with high performance and low cost is crucial for implementation of next-generation Na-ion batteries (NIBs) electrode, which is proposed to meet the challenges of large scale renewable energy storage. Metal chalcogenides are considered as promising anode materials for NIBs due to their high theoretical capacity, low cost, and abundant sources. Unfortunately, their practical application in NIBs is still hindered because of low conductivity and morphological collapse caused by their volume expansion and shrinkage during Na(+) intercalation/deintercalation. To solve the daunting challenges, herein, we fabricated novel three-dimensional (3D) Cu2NiSnS4 nanoflowers (CNTSNs) as a proof-of-concept experiment using a facile and low-cost method. Furthermore, homogeneous integration with reduced graphene oxide nanosheets (RGNs) endows intrinsically insulated CNTSNs with superior electrochemical performances, including high specific capacity (up to 837 mAh g(-1)), good rate capability, and long cycling stability, which could be attributed to the unique 3D hierarchical structure providing fast ion diffusion pathway and high contact area at the electrode/electrolyte interface.

  11. SnO2@PANI Core-Shell Nanorod Arrays on 3D Graphite Foam: A High-Performance Integrated Electrode for Lithium-Ion Batteries.

    PubMed

    Zhang, Feng; Yang, Chengkai; Gao, Xin; Chen, Shuai; Hu, Yiran; Guan, Huanqin; Ma, Yurong; Zhang, Jin; Zhou, Henghui; Qi, Limin

    2017-03-22

    The rational design and controllable fabrication of electrode materials with tailored structures and superior performance is highly desirable for the next-generation lithium ion batteries (LIBs). In this work, a novel three-dimensional (3D) graphite foam (GF)@SnO2 nanorod arrays (NRAs)@polyaniline (PANI) hybrid architecture was constructed via solvothermal growth followed by electrochemical deposition. Aligned SnO2 NRAs were uniformly grown on the surface of GF, and a PANI shell with a thickness of ∼40 nm was coated on individual SnO2 nanorods, forming a SnO2@PANI core-shell structure. Benefiting from the synergetic effect of 3D GF with large surface area and high conductivity, SnO2 NRAs offering direct pathways for electrons and lithium ions, and the conductive PANI shell that accommodates the large volume variation of SnO2, the binder-free, integrated GF@SnO2 NRAs@PANI electrode for LIBs exhibited high capacity, excellent rate capability, and good electrochemical stability. A high discharge capacity of 540 mAh g(-1) (calculated by the total mass of the electrode) was achieved after 50 cycles at a current density of 500 mA g(-1). Moreover, the electrode demonstrated superior rate performance with a discharge capacity of 414 mAh g(-1) at a high rate of 3 A g(-1).

  12. 3D modeling of the Buhi debris avalanche deposit of Iriga Volcano, Philippines by integrating shallow-seismic reflection and geological data

    NASA Astrophysics Data System (ADS)

    Minimo, Likha G.; Lagmay, Alfredo Mahar Francisco A.

    2016-06-01

    Numerical models for simulating volcanic debris avalanches commonly lack a critical initiation parameter, the source volume, which is difficult to estimate without data on the deposit thickness. This, in turn, limits how rheology can be characterized for simulating flow. Leapfrog Geo, a 3D geological modeling software, was used to integrate shallow-seismic reflection profiles with field and borehole data to determine the volume of the Buhi debris avalanche and the pre-collapse structure of Iriga Volcano. Volumes of the deposit calculated in this way are 34-71% larger than previous estimates. This technique may improve models of debris avalanches elsewhere in the world, and more precisely depict landslide runout and lateral extent, thus improving disaster prevention and mitigation for the many cities located near volcanoes.

  13. Integrating focal adhesion dynamics, cytoskeleton remodeling, and actin motor activity for predicting cell migration on 3D curved surfaces of the extracellular matrix.

    PubMed

    Kim, Min-Cheol; Kim, Choong; Wood, Levi; Neal, Devin; Kamm, Roger D; Asada, H Harry

    2012-11-01

    An integrative cell migration model incorporating focal adhesion (FA) dynamics, cytoskeleton and nucleus remodeling and actin motor activity is developed for predicting cell migration behaviors on 3-dimensional curved surfaces, such as cylindrical lumens in the 3-D extracellular matrix (ECM). The work is motivated by 3-D microfluidic migration experiments suggesting that the migration speed and direction may vary depending on the cross sectional shape of the lumen along which the cell migrates. In this paper, the mechanical structure of the cell is modeled as double elastic membranes of cell and nucleus. The two elastic membranes are connected by stress fibers, which are extended from focal adhesions on the cell surface to the nuclear membrane. The cell deforms and gains traction as transmembrane integrins distributed over the outer cell membrane bind to ligands on the ECM, form focal adhesions, and activate stress fibers. Probabilities at which integrin ligand-receptor bonds are formed as well as ruptures are affected by the surface geometry, resulting in diverse migration behaviors that depend on the curvature of the surface. Monte Carlo simulations of the integrative model reveal that (a) the cell migration speed is dependent on the cross sectional area of the lumen with a maximum speed at a particular diameter or width, (b) as the lumen diameter increases, the cell tends to spread and migrate around the circumference of the lumen, while it moves in the longitudinal direction as the lumen diameter narrows, (c) once the cell moves in one direction, it tends to stay migrating in the same direction despite the stochastic nature of migration. The relationship between the cell migration speed and the lumen width agrees with microfluidic experimental data for cancer cell migration.

  14. 3D and Education

    NASA Astrophysics Data System (ADS)

    Meulien Ohlmann, Odile

    2013-02-01

    Today the industry offers a chain of 3D products. Learning to "read" and to "create in 3D" becomes an issue of education of primary importance. 25 years professional experience in France, the United States and Germany, Odile Meulien set up a personal method of initiation to 3D creation that entails the spatial/temporal experience of the holographic visual. She will present some different tools and techniques used for this learning, their advantages and disadvantages, programs and issues of educational policies, constraints and expectations related to the development of new techniques for 3D imaging. Although the creation of display holograms is very much reduced compared to the creation of the 90ies, the holographic concept is spreading in all scientific, social, and artistic activities of our present time. She will also raise many questions: What means 3D? Is it communication? Is it perception? How the seeing and none seeing is interferes? What else has to be taken in consideration to communicate in 3D? How to handle the non visible relations of moving objects with subjects? Does this transform our model of exchange with others? What kind of interaction this has with our everyday life? Then come more practical questions: How to learn creating 3D visualization, to learn 3D grammar, 3D language, 3D thinking? What for? At what level? In which matter? for whom?

  15. Design of a single projector multiview 3D display system

    NASA Astrophysics Data System (ADS)

    Geng, Jason

    2014-03-01

    Multiview three-dimensional (3D) display is able to provide horizontal parallax to viewers with high-resolution and fullcolor images being presented to each view. Most multiview 3D display systems are designed and implemented using multiple projectors, each generating images for one view. Although this multi-projector design strategy is conceptually straightforward, implementation of such multi-projector design often leads to a very expensive system and complicated calibration procedures. Even for a multiview system with a moderate number of projectors (e.g., 32 or 64 projectors), the cost of a multi-projector 3D display system may become prohibitive due to the cost and complexity of integrating multiple projectors. In this article, we describe an optical design technique for a class of multiview 3D display systems that use only a single projector. In this single projector multiview (SPM) system design, multiple views for the 3D display are generated in a time-multiplex fashion by the single high speed projector with specially designed optical components, a scanning mirror, and a reflective mirror array. Images of all views are generated sequentially and projected via the specially design optical system from different viewing directions towards a 3D display screen. Therefore, the single projector is able to generate equivalent number of multiview images from multiple viewing directions, thus fulfilling the tasks of multiple projectors. An obvious advantage of the proposed SPM technique is the significant reduction of cost, size, and complexity, especially when the number of views is high. The SPM strategy also alleviates the time-consuming procedures for multi-projector calibration. The design method is flexible and scalable and can accommodate systems with different number of views.

  16. Compact monolithically-integrated hybrid (de)multiplexer based on silicon-on-insulator nanowires for PDM-WDM systems.

    PubMed

    Chen, Sitao; Shi, Yaocheng; He, Sailing; Dai, Daoxin

    2015-05-18

    A compact silicon hybrid (de)multiplexer is designed and demonstrated by integrating a single bi-directional AWG with a polarization diversity circuit, which consists of an ultra-short polarization-beam splitter (PBS) based on a bent coupler and a polarization rotator (PR) based on a silicon-on-insulator nanowire with a cut corner. The present hybrid (de)multiplexer can operate for both TE- and TM- polarizations and thus is available for PDM-WDM systems. An 18-channel hybrid (de)multiplexer is realized with 9 wavelengths as an example. The wavelength-channel spacing is 400GHz (i.e., Δλ(ch) = 3.2nm) and the footprint of the device is about 530μm × 210μm. The channel crosstalk is about -13dB and the total excess loss is about 7dB. The excess loss increases by about 1~2dB due to the cascaded polarization diversity circuit in comparison with a single bi-directional AWG.

  17. A platform for multiplexed sensing of biomolecules using high-Q microring resonator arrays with differential readout and integrated microfluidics

    NASA Astrophysics Data System (ADS)

    Wright, J. B.; Brener, I.; Westlake, K. R.; Branch, D. W.; Shaw, M. J.; Vawter, G. A.

    2010-02-01

    We demonstrate chemical/biological sensor arrays based on high quality factor evanescent microring waveguide resonators in a process that is compatible with CMOS fabrication, glass microfluidic integration, and robust surface chemistry ligand attachment. We cancel out any fluctuations due to liquid temperature variations through a differential dual sensor design. Using laser locking servo techniques we attain detection sensitivities in the ng/ml range. This combination of silicon photonic sensors, robust packaging, high sensitivity and arrayed design is capable of providing a platform for multiplexed chem-bio sensing of molecules suspended in solution.

  18. In-band OSNR monitor with high-speed integrated Stokes polarimeter for polarization division multiplexed signal.

    PubMed

    Saida, Takashi; Ogawa, Ikuo; Mizuno, Takayuki; Sano, Kimikazu; Fukuyama, Hiroyuki; Muramoto, Yoshifumi; Hashizume, Yasuaki; Nosaka, Hideyuki; Yamamoto, Shuto; Murata, Koichi

    2012-12-10

    An in-band optical signal-to-noise ratio (OSNR) monitor is proposed, based on an instantaneous polarization state distribution analysis. The proposed monitor is simple, and is applicable to polarization division multiplexed signals. We fabricate a high-speed Stokes polarimeter that integrates a planar lightwave circuit (PLC) based polarization filter, high-speed InP/InGaAs photodiodes and InP hetero-junction bipolar transistor (HBT) trans-impedance amplifiers (TIA). We carry out proof-of-concept experiments with the fabricated polarimeter, and successfully measure the OSNR dependent polarization distribution with 100-Gb/s dual polarization quadrature phase shift keying (DP-QPSK) signals.

  19. 4D ICE: A 2D Array Transducer with Integrated ASIC in a 10 Fr Catheter for Real-Time 3D Intracardiac Echocardiography.

    PubMed

    Wildes, Douglas; Lee, Warren; Haider, Bruno; Cogan, Scott; Sundaresan, Krishnakumar; Mills, David; Yetter, Christopher; Hart, Patrick; Haun, Christopher; Concepcion, Mikael; Kirkhorn, Johan; Bitoun, Marc

    2016-10-12

    We developed a 2.5 x 6.6 mm 2D array transducer with integrated transmit/receive ASIC for 4D ICE (real-time 3D IntraCardiac Echocardiography) applications. The ASIC and transducer design were optimized so that the high voltage transmit, low-voltage TGC (time-gain control) and preamp, subaperture beamformer, and digital control circuits for each transducer element all fit within the 0.019 mm2 area of the element. The transducer assembly was deployed in a 10 Fr (3.3 mm diameter) catheter, integrated with a GE Vivid1 E9 ultrasound imaging system, and evaluated in three pre-clinical studies. 2D image quality and imaging modes were comparable to commercial 2D ICE catheters. The 4D field of view was at least 90° x 60° x 8 cm and could be imaged at 30 volumes/sec, sufficient to visualize cardiac anatomy and other diagnostic and therapy catheters. 4D ICE should significantly reduce X-ray fluoroscopy use and dose during electrophysiology (EP) ablation procedures. 4D ICE may be able to replace trans-esophageal echocardiography (TEE), and the associated risks and costs of general anesthesia, for guidance of some structural heart procedures.

  20. 3-D Extensions for Trustworthy Systems

    DTIC Science & Technology

    2011-01-01

    3- D Extensions for Trustworthy Systems (Invited Paper) Ted Huffmire∗, Timothy Levin∗, Cynthia Irvine∗, Ryan Kastner† and Timothy Sherwood...address these problems, we propose an approach to trustworthy system development based on 3- D integration, an emerging chip fabrication technique in...which two or more integrated circuit dies are fabricated individually and then combined into a single stack using vertical conductive posts. With 3- D

  1. 3D Imaging.

    ERIC Educational Resources Information Center

    Hastings, S. K.

    2002-01-01

    Discusses 3 D imaging as it relates to digital representations in virtual library collections. Highlights include X-ray computed tomography (X-ray CT); the National Science Foundation (NSF) Digital Library Initiatives; output peripherals; image retrieval systems, including metadata; and applications of 3 D imaging for libraries and museums. (LRW)

  2. Building the 3D Geological Model of Wall Rock of Salt Caverns Based on Integration Method of Multi-source data

    NASA Astrophysics Data System (ADS)

    Yongzhi, WANG; hui, WANG; Lixia, LIAO; Dongsen, LI

    2017-02-01

    In order to analyse the geological characteristics of salt rock and stability of salt caverns, rough three-dimensional (3D) models of salt rock stratum and the 3D models of salt caverns on study areas are built by 3D GIS spatial modeling technique. During implementing, multi-source data, such as basic geographic data, DEM, geological plane map, geological section map, engineering geological data, and sonar data are used. In this study, the 3D spatial analyzing and calculation methods, such as 3D GIS intersection detection method in three-dimensional space, Boolean operations between three-dimensional space entities, three-dimensional space grid discretization, are used to build 3D models on wall rock of salt caverns. Our methods can provide effective calculation models for numerical simulation and analysis of the creep characteristics of wall rock in salt caverns.

  3. 3D Printed Bionic Nanodevices.

    PubMed

    Kong, Yong Lin; Gupta, Maneesh K; Johnson, Blake N; McAlpine, Michael C

    2016-06-01

    The ability to three-dimensionally interweave biological and functional materials could enable the creation of bionic devices possessing unique and compelling geometries, properties, and functionalities. Indeed, interfacing high performance active devices with biology could impact a variety of fields, including regenerative bioelectronic medicines, smart prosthetics, medical robotics, and human-machine interfaces. Biology, from the molecular scale of DNA and proteins, to the macroscopic scale of tissues and organs, is three-dimensional, often soft and stretchable, and temperature sensitive. This renders most biological platforms incompatible with the fabrication and materials processing methods that have been developed and optimized for functional electronics, which are typically planar, rigid and brittle. A number of strategies have been developed to overcome these dichotomies. One particularly novel approach is the use of extrusion-based multi-material 3D printing, which is an additive manufacturing technology that offers a freeform fabrication strategy. This approach addresses the dichotomies presented above by (1) using 3D printing and imaging for customized, hierarchical, and interwoven device architectures; (2) employing nanotechnology as an enabling route for introducing high performance materials, with the potential for exhibiting properties not found in the bulk; and (3) 3D printing a range of soft and nanoscale materials to enable the integration of a diverse palette of high quality functional nanomaterials with biology. Further, 3D printing is a multi-scale platform, allowing for the incorporation of functional nanoscale inks, the printing of microscale features, and ultimately the creation of macroscale devices. This blending of 3D printing, novel nanomaterial properties, and 'living' platforms may enable next-generation bionic systems. In this review, we highlight this synergistic integration of the unique properties of nanomaterials with the

  4. Multiplexed Simultaneous High Sensitivity Sensors with High-Order Mode Based on the Integration of Photonic Crystal 1 × 3 Beam Splitter and Three Different Single-Slot PCNCs

    PubMed Central

    Zhou, Jian; Huang, Lijun; Fu, Zhongyuan; Sun, Fujun; Tian, Huiping

    2016-01-01

    We simulated an efficient method for the sensor array of high-sensitivity single-slot photonic crystal nanobeam cavities (PCNCs) on a silicon platform. With the combination of a well-designed photonic crystal waveguide (PhCW) filter and an elaborate single-slot PCNC, a specific high-order resonant mode was filtered for sensing. A 1 × 3 beam splitter carefully established was implemented to split channels and integrate three sensors to realize microarrays. By applying the three-dimensional finite-difference-time-domain (3D-FDTD) method, the sensitivities calculated were S1 = 492 nm/RIU, S2 = 244 nm/RIU, and S3 = 552 nm/RIU, respectively. To the best of our knowledge, this is the first multiplexing design in which each sensor cite features such a high sensitivity simultaneously. PMID:27399712

  5. Multiplexed Simultaneous High Sensitivity Sensors with High-Order Mode Based on the Integration of Photonic Crystal 1 × 3 Beam Splitter and Three Different Single-Slot PCNCs.

    PubMed

    Zhou, Jian; Huang, Lijun; Fu, Zhongyuan; Sun, Fujun; Tian, Huiping

    2016-07-07

    We simulated an efficient method for the sensor array of high-sensitivity single-slot photonic crystal nanobeam cavities (PCNCs) on a silicon platform. With the combination of a well-designed photonic crystal waveguide (PhCW) filter and an elaborate single-slot PCNC, a specific high-order resonant mode was filtered for sensing. A 1 × 3 beam splitter carefully established was implemented to split channels and integrate three sensors to realize microarrays. By applying the three-dimensional finite-difference-time-domain (3D-FDTD) method, the sensitivities calculated were S₁ = 492 nm/RIU, S₂ = 244 nm/RIU, and S₃ = 552 nm/RIU, respectively. To the best of our knowledge, this is the first multiplexing design in which each sensor cite features such a high sensitivity simultaneously.

  6. Fast and Broadband Signal Integrity Analysis of Multiple Vias in Heterogeneous 3D IC and Die-Level Packaging by Using Generalized Foldy-Lax Scattering Method

    NASA Astrophysics Data System (ADS)

    Chang, Xin

    This dissertation proposal is concerned with the use of fast and broadband full-wave electromagnetic methods for modeling high speed interconnects (e.g, vertical vias and horizontal traces) and passive components (e.g, decoupling capacitors) for structures of PCB and packages, in 3D IC, Die-level packaging and SIW based devices, to effectively modeling the designs signal integrity (SI) and power integrity (PI) aspects. The main contributions finished in this thesis is to create a novel methodology, which hybridizes the Foldy-Lax multiple scattering equations based fast full wave method, method of moment (MoM) based 1D technology, modes decoupling based geometry decomposition and cavity modes expansions, to model and simulate the electromagnetic scattering effects for the irregular power/ground planes, multiple vias and traces, for fast and accurate analysis of link level simulation on multilayer electronic structures. For the modeling details, the interior massively-coupled multiple vias problem is modeled most-analytically by using the Foldy-Lax multiple scattering equations. The dyadic Green's functions of the magnetic field are expressed in terms of waveguide modes in the vertical direction and vector cylindrical wave expansions or cavity modes expansions in the horizontal direction, combined with 2D MoM realized by 1D technology. For the incident field of the case of vias in the arbitrarily shaped antipad in finite large cavity/waveguide, the exciting and scattering field coefficients are calculated based on the transformation which converts surface integration of magnetic surface currents in antipad into 1D line integration of surface charges on the vias and on the ground plane. Geometry decomposition method is applied to model and integrate both the vertical and horizontal interconnects/traces in arbitrarily shaped power/ground planes. Moreover, a new form of multiple scattering equations is derived for solving coupling effects among mixed metallic

  7. Increment of Access Points in Integrated System of Wavelength Division Multiplexed Passive Optical Network Radio over Fiber

    PubMed Central

    Amiri, I. S.; Alavi, S. E.; Soltanian, M. R. K.; Fisal, N.; Supa’at, A. S. M.; Ahmad, H.

    2015-01-01

    This paper describes a novel technique to increase the numbers of access points (APs) in a wavelength division multiplexed-passive optical network (WDM-PON) integrated in a 100 GHz radio-over-fiber (RoF). Eight multi-carriers separated by 25 GHz intervals were generated in the range of 193.025 to 193.200 THz using a microring resonator (MRR) system incorporating an add-drop filter system. All optically generated multi-carriers were utilized in an integrated system of WDM-PON-RoF for transmission of four 43.6 Gb/sec orthogonal frequency division multiplexing (OFDM) signals. Results showed that an acceptable BER variation for different path lengths up to 25 km was achievable for all four access points and thus the transmission of four OFDM channels is feasible for a 25 km standard single mode fiber (SSMF) path length. PMID:26153536

  8. Automation and integration of multiplexed on-line sample preparation with capillary electrophoresis for DNA sequencing

    SciTech Connect

    Tan, H.

    1999-03-31

    The purpose of this research is to develop a multiplexed sample processing system in conjunction with multiplexed capillary electrophoresis for high-throughput DNA sequencing. The concept from DNA template to called bases was first demonstrated with a manually operated single capillary system. Later, an automated microfluidic system with 8 channels based on the same principle was successfully constructed. The instrument automatically processes 8 templates through reaction, purification, denaturation, pre-concentration, injection, separation and detection in a parallel fashion. A multiplexed freeze/thaw switching principle and a distribution network were implemented to manage flow direction and sample transportation. Dye-labeled terminator cycle-sequencing reactions are performed in an 8-capillary array in a hot air thermal cycler. Subsequently, the sequencing ladders are directly loaded into a corresponding size-exclusion chromatographic column operated at {approximately} 60 C for purification. On-line denaturation and stacking injection for capillary electrophoresis is simultaneously accomplished at a cross assembly set at {approximately} 70 C. Not only the separation capillary array but also the reaction capillary array and purification columns can be regenerated after every run. DNA sequencing data from this system allow base calling up to 460 bases with accuracy of 98%.

  9. High-κ Al{sub 2}O{sub 3} material in low temperature wafer-level bonding for 3D integration application

    SciTech Connect

    Fan, J. Tu, L. C.; Tan, C. S.

    2014-03-15

    This work systematically investigated a high-κ Al{sub 2}O{sub 3} material for low temperature wafer-level bonding for potential applications in 3D microsystems. A clean Si wafer with an Al{sub 2}O{sub 3} layer thickness of 50 nm was applied as our experimental approach. Bonding was initiated in a clean room ambient after surface activation, followed by annealing under inert ambient conditions at 300 °C for 3 h. The investigation consisted of three parts: a mechanical support study using the four-point bending method, hermeticity measurements using the helium bomb test, and thermal conductivity analysis for potential heterogeneous bonding. Compared with samples bonded using a conventional oxide bonding material (SiO{sub 2}), a higher interfacial adhesion energy (∼11.93 J/m{sup 2}) and a lower helium leak rate (∼6.84 × 10{sup −10} atm.cm{sup 3}/sec) were detected for samples bonded using Al{sub 2}O{sub 3}. More importantly, due to the excellent thermal conductivity performance of Al{sub 2}O{sub 3}, this technology can be used in heterogeneous direct bonding, which has potential applications for enhancing the performance of Si photonic integrated devices.

  10. Progress Toward an Integration of Process-Structure-Property-Performance Models for "Three-Dimensional (3-D) Printing" of Titanium Alloys

    NASA Astrophysics Data System (ADS)

    Collins, P. C.; Haden, C. V.; Ghamarian, I.; Hayes, B. J.; Ales, T.; Penso, G.; Dixit, V.; Harlow, G.

    2014-07-01

    Electron beam direct manufacturing, synonymously known as electron beam additive manufacturing, along with other additive "3-D printing" manufacturing processes, are receiving widespread attention as a means of producing net-shape (or near-net-shape) components, owing to potential manufacturing benefits. Yet, materials scientists know that differences in manufacturing processes often significantly influence the microstructure of even widely accepted materials and, thus, impact the properties and performance of a material in service. It is important to accelerate the understanding of the processing-structure-property relationship of materials being produced via these novel approaches in a framework that considers the performance in a statistically rigorous way. This article describes the development of a process model, the assessment of key microstructural features to be incorporated into a microstructure simulation model, a novel approach to extract a constitutive equation to predict tensile properties in Ti-6Al-4V (Ti-64), and a probabilistic approach to measure the fidelity of the property model against real data. This integrated approach will provide designers a tool to vary process parameters and understand the influence on performance, enabling design and optimization for these highly visible manufacturing approaches.

  11. Oblique incidence of semi-guided waves on step-like folds in planar dielectric slabs: Lossless vertical interconnects in 3D integrated photonic circuits

    NASA Astrophysics Data System (ADS)

    Hildebrandt, Andre; Alhaddad, Samer; Hammer, Manfred; Förstner, Jens

    2016-02-01

    Semi-guided light propagation across linear folds of slab waveguides is being considered. Radiation losses vanish beyond certain critical angles of incidence, as can be understood by arguments resembling Snell's law. One thus realizes lossless propagation through 90-degree corner configurations, where the remaining guided waves are still subject to pronounced reflection and polarization conversion. A step-like system of two of these sharp corners can then be viewed as a system akin to a Fabry-Perot interferometer, with two partial reflectors at a distance given by the vertical separation of the slab cores. The respective resonance effect enables full transmission of semiguided, laterally plane waves through the step structures. One obtains a configuration that optically connects guiding layers at different elevation levels in a 3-D integrated optical chip, without radiation losses, over large distances, and reasonably broadband. We show rigorous quasi-analytical results for typical high-contrast Si/SiO2 structures. Although the full-transmission effect requires a symmetric system, here realized by slab waveguides with a silicon core sandwiched between thick silica substrate and cover layers, simulations for configurations with air cover show that a certain asymmetry can well be afforded.

  12. Derivation of 3-D surface deformation from an integration of InSAR and GNSS measurements based on Akaike's Bayesian Information Criterion

    NASA Astrophysics Data System (ADS)

    Luo, Haipeng; Liu, Yang; Chen, Ting; Xu, Caijun; Wen, Yangmao

    2016-01-01

    We present a new method to derive 3-D surface deformation from an integration of interferometric synthetic aperture radar (InSAR) images and Global Navigation Satellite System (GNSS) observations based on Akaike's Bayesian Information Criterion (ABIC), considering relationship between deformations of neighbouring locations. This method avoids interpolated errors by excluding the interpolation of GNSS into the same spatial resolution as InSAR images and harnesses the data sets and the prior smooth constraints of surface deformation objectively and simultaneously by using ABIC, which were inherently unresolved in previous studies. In particular, we define surface roughness measuring smoothing degree to evaluate the performance of the prior constraints and deduce the formula of the covariance for the estimation errors to estimate the uncertainty of modelled solution. We validate this method using synthetic tests and the 2008 Mw 7.9 Wenchuan earthquake. We find that the optimal weights associated with ABIC minimum are generally at trade-off locations that balance contributions from InSAR, GNSS data sets and the prior constraints. We use this method to evaluate the influence of the interpolated errors from the Ordinary Kriging algorithm on the derivation of surface deformation. Tests show that the interpolated errors may contribute to biasing very large weights imposed on Kriged GNSS data, suggesting that fixing the relative weights is required in this case. We also make a comparison with SISTEM method, indicating that our method allows obtaining better estimations even with sparse GNSS observations. In addition, this method can be generalized to provide a solution for situations where some types of data sets are lacking and can be exploited further to account for data sets such as the integration of displacements along radar lines and offsets along satellite tracks.

  13. Complex angular momentum theory of state-to-state integral cross sections: resonance effects in the F + HD → HF(v' = 3) + D reaction.

    PubMed

    Sokolovski, D; Akhmatskaya, E; Echeverría-Arrondo, C; De Fazio, D

    2015-07-28

    State-to-state reactive integral cross sections (ICSs) are often affected by quantum mechanical resonances, especially near a reactive threshold. An ICS is usually obtained by summing partial waves at a given value of energy. For this reason, the knowledge of pole positions and residues in the complex energy plane is not sufficient for a quantitative description of the patterns produced by resonance. Such description is available in terms of the poles of an S-matrix element in the complex plane of the total angular momentum. The approach was recently implemented in a computer code ICS_Regge, available in the public domain [Comput. Phys. Commun., 2014, 185, 2127]. In this paper, we employ the ICS_Regge package to analyse in detail, for the first time, the resonance patterns predicted for integral cross sections (ICSs) of the benchmark F + HD → HF(v' = 3) + D reaction. The v = 0, j = 0, Ω = 0 → v' = 3, j' = 0, 1, 2, and Ω' = 0, 1, 2 transitions are studied for collision energies from 58.54 to 197.54 meV. For these energies, we find several resonances, whose contributions to the ICS vary from symmetric and asymmetric Fano shapes to smooth sinusoidal Regge oscillations. Complex energies of metastable states and Regge pole positions and residues are found by Padé reconstruction of the scattering matrix elements. The accuracy of the ICS_Regge code, relation between complex energies and Regge poles, various types of Regge trajectories, and the origin of the J-shifting approximation are also discussed.

  14. Microfluidic biosensor array with integrated poly(2,7-carbazole)/fullerene-based photodiodes for rapid multiplexed detection of pathogens.

    PubMed

    Matos Pires, Nuno Miguel; Dong, Tao

    2013-11-25

    A multiplexed microfluidic biosensor made of poly(methylmethacrylate) (PMMA) was integrated into an array of organic blend heterojunction photodiodes (OPDs) for chemiluminescent detection of pathogens. Waterborne Escherichia coli O157:H7, Campylobacter jejuni and adenovirus were targeted in the PMMA chip, and detection of captured pathogens was conducted by poly(2,7-carbazole)/fullerene OPDs which showed a responsivity over 0.20 A/W at 425 nm. The limits of chemiluminescent detection were 5 × 10(5) cells/mL for E. coli, 1 × 10(5) cells/mL for C. jejuni, and 1 × 10(-8) mg/mL for adenovirus. Parallel analysis for all three analytes in less than 35 min was demonstrated. Further recovery tests illustrated the potential of the integrated biosensor for detecting bacteria in real water samples.

  15. The Effects of Realistic Synaptic Distribution and 3D Geometry on Signal Integration and Extracellular Field Generation of Hippocampal Pyramidal Cells and Inhibitory Neurons

    PubMed Central

    Gulyás, Attila I.; Freund, Tamás F.; Káli, Szabolcs

    2016-01-01

    In vivo and in vitro multichannel field and somatic intracellular recordings are frequently used to study mechanisms of network pattern generation. When interpreting these data, neurons are often implicitly considered as electrotonically compact cylinders with a homogeneous distribution of excitatory and inhibitory inputs. However, the actual distributions of dendritic length, diameter, and the densities of excitatory and inhibitory input are non-uniform and cell type-specific. We first review quantitative data on the dendritic structure and synaptic input and output distribution of pyramidal cells (PCs) and interneurons in the hippocampal CA1 area. Second, using multicompartmental passive models of four different types of neurons, we quantitatively explore the effect of differences in dendritic structure and synaptic distribution on the errors and biases of voltage clamp measurements of inhibitory and excitatory postsynaptic currents. Finally, using the 3-dimensional distribution of dendrites and synaptic inputs we calculate how different inhibitory and excitatory inputs contribute to the generation of local field potential in the hippocampus. We analyze these effects at different realistic background activity levels as synaptic bombardment influences neuronal conductance and thus the propagation of signals in the dendritic tree. We conclude that, since dendrites are electrotonically long and entangled in 3D, somatic intracellular and field potential recordings miss the majority of dendritic events in some cell types, and thus overemphasize the importance of perisomatic inhibitory inputs and belittle the importance of complex dendritic processing. Modeling results also suggest that PCs and inhibitory neurons probably use different input integration strategies. In PCs, second- and higher-order thin dendrites are relatively well-isolated from each other, which may support branch-specific local processing as suggested by studies of active dendritic integration. In

  16. The Effects of Realistic Synaptic Distribution and 3D Geometry on Signal Integration and Extracellular Field Generation of Hippocampal Pyramidal Cells and Inhibitory Neurons.

    PubMed

    Gulyás, Attila I; Freund, Tamás F; Káli, Szabolcs

    2016-01-01

    In vivo and in vitro multichannel field and somatic intracellular recordings are frequently used to study mechanisms of network pattern generation. When interpreting these data, neurons are often implicitly considered as electrotonically compact cylinders with a homogeneous distribution of excitatory and inhibitory inputs. However, the actual distributions of dendritic length, diameter, and the densities of excitatory and inhibitory input are non-uniform and cell type-specific. We first review quantitative data on the dendritic structure and synaptic input and output distribution of pyramidal cells (PCs) and interneurons in the hippocampal CA1 area. Second, using multicompartmental passive models of four different types of neurons, we quantitatively explore the effect of differences in dendritic structure and synaptic distribution on the errors and biases of voltage clamp measurements of inhibitory and excitatory postsynaptic currents. Finally, using the 3-dimensional distribution of dendrites and synaptic inputs we calculate how different inhibitory and excitatory inputs contribute to the generation of local field potential in the hippocampus. We analyze these effects at different realistic background activity levels as synaptic bombardment influences neuronal conductance and thus the propagation of signals in the dendritic tree. We conclude that, since dendrites are electrotonically long and entangled in 3D, somatic intracellular and field potential recordings miss the majority of dendritic events in some cell types, and thus overemphasize the importance of perisomatic inhibitory inputs and belittle the importance of complex dendritic processing. Modeling results also suggest that PCs and inhibitory neurons probably use different input integration strategies. In PCs, second- and higher-order thin dendrites are relatively well-isolated from each other, which may support branch-specific local processing as suggested by studies of active dendritic integration. In

  17. AE3D

    SciTech Connect

    Spong, Donald A

    2016-06-20

    AE3D solves for the shear Alfven eigenmodes and eigenfrequencies in a torodal magnetic fusion confinement device. The configuration can be either 2D (e.g. tokamak, reversed field pinch) or 3D (e.g. stellarator, helical reversed field pinch, tokamak with ripple). The equations solved are based on a reduced MHD model and sound wave coupling effects are not currently included.

  18. Wired/wireless access integrated RoF-PON with scalable generation of multi-frequency MMWs enabled by polarization multiplexed FWM in SOA.

    PubMed

    Xiang, Yu; Chen, Chen; Zhang, Chongfu; Qiu, Kun

    2013-01-14

    In this paper, we propose and demonstrate a novel integrated radio-over-fiber passive optical network (RoF-PON) system for both wired and wireless access. By utilizing the polarization multiplexed four-wave mixing (FWM) effect in a semiconductor optical amplifier (SOA), scalable generation of multi-frequency millimeter-waves (MMWs) can be provided so as to assist the configuration of multi-frequency wireless access for the wire/wireless access integrated ROF-PON system. In order to obtain a better performance, the polarization multiplexed FWM effect is investigated in detail. Simulation results successfully verify the feasibility of our proposed scheme.

  19. Upper Crustal Structure above Off-axis Magma Lenses at RIDGE-2000 East Pacific Rise Integrated Study Site from 3D Multichannel Seismic Reflection Data

    NASA Astrophysics Data System (ADS)

    Han, S.; Carbotte, S. M.; Carton, H. D.; Newman, K. R.; Canales, J.; Nedimovic, M. R.

    2010-12-01

    The 2008 multi-streamer 3D seismic reflection experiment conducted aboard the R/V Marcus Langseth at the RIDGE-2000 East Pacific Rise Integrated Study Site reveals prominent near-axis crustal reflectors on both the east and west flanks of the ridge crest which are interpreted as off-axis melt lenses (OAML) injected at mid-crustal levels. These OAML are probable sites of off-axis volcanism and provide potential heat sources for localized hydrothermal circulation on the ridge flanks, which we speculate may affect off-axis upper crustal structure. To investigate the effect of OAML on the upper crustal structure, we choose two across-axis lines above a prominent OAML on the east flank of the ridge that is present in the southernmost part of our study area: Line 1428P across the middle part of the OAML near 9° 38’N and Line 1476P across the northern end of this OAML near 9° 39’N. Initial analysis includes 2D processing to produce seismic reflection images for each line and 1D travel time modeling on CMP super gathers to characterize Layer 2A and upper Layer 2B velocity structure. Comparison of seismic reflection images and upper crustal velocity structure for the two lines shows a decrease in Layer 2A thickness by 150m and a decrease in the uppermost 2B velocity by 10-20% above the central portion of OAML. We attribute these local anomalies to alteration associated with off-axis hydrothermal circulation driven by the OAML where enhanced precipitation of alteration minerals may seal porosity within lowermost Layer 2A, converting it to lower velocity uppermost Layer 2B. To further constrain the velocity structure of Layer 2A and Layer 2B, we conduct 2D P-wave tomography with downward continued shot gathers along the studied lines (Harding et al, 2007). The downward continued shot gathers simulate seismic sources and receivers located near the seafloor, and therefore provide travel time information from near-offset refractions that are normally obscured by the

  20. Development of an integrated endoscopic device for multiplexed low coherence interferometry measurements of microbicide gel coating thickness

    NASA Astrophysics Data System (ADS)

    Drake, Tyler K.; Robles, Francisco E.; DeSoto, Michael; Henderson, Marcus H.; Katz, David F.; Wax, Adam P.

    2009-02-01

    Microbicide gels are topical products that have recently been developed to combat sexually transmitted diseases including HIV/AIDS. The extent of gel coverage, thickness, and structure are crucial factors in gel effectiveness. It is necessary to be able to monitor gel distribution and behavior under various circumstances, such as coatis, and over an extended time scale in vivo. We have developed a multiplexed, Fourier-domain low coherence interferometry (LCI) system as a practical method of measuring microbicide gel distribution, with precision and accuracy comparable to currently used fluorometric techniques techniques. The multiplexed system achieved a broad scanning area without the need for a mechanical scanning device, typical of OCT systems, by utilizing six parallel channels with simultaneous data collection. We now propose an imaging module which will allow the integration of the multiplexed LCI system into the current fluorescence system in conjunction with an endoscope. The LCI imaging module will meet several key criteria in order to be compatible with the current system. The fluorescent system features a 4-mm diameter rigid endsoscope enclosed in a 27-mm diameter polycarbonate tube, with a water immersion tip. Therefore, the LCI module must be low-profile as well as water-resistant to fit inside the current design. It also must fulfill its primary function of delivering light from each of the six channels to the gel and collecting backscattered light. The performance of the imaging module will be characterized by scanning a calibration socket which contains grooves of known depths, and comparing these measurements to the fluorometric results.

  1. FastScript3D - A Companion to Java 3D

    NASA Technical Reports Server (NTRS)

    Koenig, Patti

    2005-01-01

    FastScript3D is a computer program, written in the Java 3D(TM) programming language, that establishes an alternative language that helps users who lack expertise in Java 3D to use Java 3D for constructing three-dimensional (3D)-appearing graphics. The FastScript3D language provides a set of simple, intuitive, one-line text-string commands for creating, controlling, and animating 3D models. The first word in a string is the name of a command; the rest of the string contains the data arguments for the command. The commands can also be used as an aid to learning Java 3D. Developers can extend the language by adding custom text-string commands. The commands can define new 3D objects or load representations of 3D objects from files in formats compatible with such other software systems as X3D. The text strings can be easily integrated into other languages. FastScript3D facilitates communication between scripting languages [which enable programming of hyper-text markup language (HTML) documents to interact with users] and Java 3D. The FastScript3D language can be extended and customized on both the scripting side and the Java 3D side.

  2. Integrated cladding-pumped multicore few-mode erbium-doped fibre amplifier for space-division-multiplexed communications

    NASA Astrophysics Data System (ADS)

    Chen, H.; Jin, C.; Huang, B.; Fontaine, N. K.; Ryf, R.; Shang, K.; Grégoire, N.; Morency, S.; Essiambre, R.-J.; Li, G.; Messaddeq, Y.; Larochelle, S.

    2016-08-01

    Space-division multiplexing (SDM), whereby multiple spatial channels in multimode and multicore optical fibres are used to increase the total transmission capacity per fibre, is being investigated to avert a data capacity crunch and reduce the cost per transmitted bit. With the number of channels employed in SDM transmission experiments continuing to rise, there is a requirement for integrated SDM components that are scalable. Here, we demonstrate a cladding-pumped SDM erbium-doped fibre amplifier (EDFA) that consists of six uncoupled multimode erbium-doped cores. Each core supports three spatial modes, which enables the EDFA to amplify a total of 18 spatial channels (six cores × three modes) simultaneously with a single pump diode and a complexity similar to a single-mode EDFA. The amplifier delivers >20 dBm total output power per core and <7 dB noise figure over the C-band. This cladding-pumped EDFA enables combined space-division and wavelength-division multiplexed transmission over multiple multimode fibre spans.

  3. Photonic integration enabling new multiplexing concepts in optical board-to-board and rack-to-rack interconnects

    NASA Astrophysics Data System (ADS)

    Apostolopoulos, Dimitrios; Bakopoulos, Paraskevas; Kalavrouziotis, Dimitrios; Giannoulis, Giannis; Kanakis, Giannis; Iliadis, Nikos; Spatharakis, Christos; Bauwelinck, Johan; Avramopoulos, Hercules

    2014-03-01

    New broadband applications are causing the datacenters to proliferate, raising the bar for higher interconnection speeds. So far, optical board-to-board and rack-to-rack interconnects relied primarily on low-cost commodity optical components assembled in a single package. Although this concept proved successful in the first generations of opticalinterconnect modules, scalability is a daunting issue as signaling rates extend beyond 25 Gb/s. In this paper we present our work towards the development of two technology platforms for migration beyond Infiniband enhanced data rate (EDR), introducing new concepts in board-to-board and rack-to-rack interconnects. The first platform is developed in the framework of MIRAGE European project and relies on proven VCSEL technology, exploiting the inherent cost, yield, reliability and power consumption advantages of VCSELs. Wavelength multiplexing, PAM-4 modulation and multi-core fiber (MCF) multiplexing are introduced by combining VCSELs with integrated Si and glass photonics as well as BiCMOS electronics. An in-plane MCF-to-SOI interface is demonstrated, allowing coupling from the MCF cores to 340x400 nm Si waveguides. Development of a low-power VCSEL driver with integrated feed-forward equalizer is reported, allowing PAM-4 modulation of a bandwidth-limited VCSEL beyond 25 Gbaud. The second platform, developed within the frames of the European project PHOXTROT, considers the use of modulation formats of increased complexity in the context of optical interconnects. Powered by the evolution of DSP technology and towards an integration path between inter and intra datacenter traffic, this platform investigates optical interconnection system concepts capable to support 16QAM 40GBd data traffic, exploiting the advancements of silicon and polymer technologies.

  4. Novel System for Real-Time Integration of 3-D Echocardiography and Fluoroscopy for Image-Guided Cardiac Interventions: Preclinical Validation and Clinical Feasibility Evaluation

    PubMed Central

    Housden, R. James; Ma, Yingliang; Rajani, Ronak; Gao, Gang; Nijhof, Niels; Cathier, Pascal; Bullens, Roland; Gijsbers, Geert; Parish, Victoria; Kapetanakis, Stamatis; Hancock, Jane; Rinaldi, C. Aldo; Cooklin, Michael; Gill, Jaswinder; Thomas, Martyn; O'neill, Mark D.; Razavi, Reza; Rhode, Kawal S.

    2014-01-01

    Real-time imaging is required to guide minimally invasive catheter-based cardiac interventions. While transesophageal echocardiography allows for high-quality visualization of cardiac anatomy, X-ray fluoroscopy provides excellent visualization of devices. We have developed a novel image fusion system that allows real-time integration of 3-D echocardiography and the X-ray fluoroscopy. The system was validated in the following two stages: 1) preclinical to determine function and validate accuracy; and 2) in the clinical setting to assess clinical workflow feasibility and determine overall system accuracy. In the preclinical phase, the system was assessed using both phantom and porcine experimental studies. Median 2-D projection errors of 4.5 and 3.3 mm were found for the phantom and porcine studies, respectively. The clinical phase focused on extending the use of the system to interventions in patients undergoing either atrial fibrillation catheter ablation (CA) or transcatheter aortic valve implantation (TAVI). Eleven patients were studied with nine in the CA group and two in the TAVI group. Successful real-time view synchronization was achieved in all cases with a calculated median distance error of 2.2 mm in the CA group and 3.4 mm in the TAVI group. A standard clinical workflow was established using the image fusion system. These pilot data confirm the technical feasibility of accurate real-time echo-fluoroscopic image overlay in clinical practice, which may be a useful adjunct for real-time guidance during interventional cardiac procedures. PMID:27170872

  5. 3-D Seismic Interpretation

    NASA Astrophysics Data System (ADS)

    Moore, Gregory F.

    2009-05-01

    This volume is a brief introduction aimed at those who wish to gain a basic and relatively quick understanding of the interpretation of three-dimensional (3-D) seismic reflection data. The book is well written, clearly illustrated, and easy to follow. Enough elementary mathematics are presented for a basic understanding of seismic methods, but more complex mathematical derivations are avoided. References are listed for readers interested in more advanced explanations. After a brief introduction, the book logically begins with a succinct chapter on modern 3-D seismic data acquisition and processing. Standard 3-D acquisition methods are presented, and an appendix expands on more recent acquisition techniques, such as multiple-azimuth and wide-azimuth acquisition. Although this chapter covers the basics of standard time processing quite well, there is only a single sentence about prestack depth imaging, and anisotropic processing is not mentioned at all, even though both techniques are now becoming standard.

  6. Radiochromic 3D Detectors

    NASA Astrophysics Data System (ADS)

    Oldham, Mark

    2015-01-01

    Radiochromic materials exhibit a colour change when exposed to ionising radiation. Radiochromic film has been used for clinical dosimetry for many years and increasingly so recently, as films of higher sensitivities have become available. The two principle advantages of radiochromic dosimetry include greater tissue equivalence (radiologically) and the lack of requirement for development of the colour change. In a radiochromic material, the colour change arises direct from ionising interactions affecting dye molecules, without requiring any latent chemical, optical or thermal development, with important implications for increased accuracy and convenience. It is only relatively recently however, that 3D radiochromic dosimetry has become possible. In this article we review recent developments and the current state-of-the-art of 3D radiochromic dosimetry, and the potential for a more comprehensive solution for the verification of complex radiation therapy treatments, and 3D dose measurement in general.

  7. Bootstrapping 3D fermions

    DOE PAGES

    Iliesiu, Luca; Kos, Filip; Poland, David; ...

    2016-03-17

    We study the conformal bootstrap for a 4-point function of fermions <ψψψψ> in 3D. We first introduce an embedding formalism for 3D spinors and compute the conformal blocks appearing in fermion 4-point functions. Using these results, we find general bounds on the dimensions of operators appearing in the ψ × ψ OPE, and also on the central charge CT. We observe features in our bounds that coincide with scaling dimensions in the GrossNeveu models at large N. Finally, we also speculate that other features could coincide with a fermionic CFT containing no relevant scalar operators.

  8. Bootstrapping 3D fermions

    SciTech Connect

    Iliesiu, Luca; Kos, Filip; Poland, David; Pufu, Silviu S.; Simmons-Duffin, David; Yacoby, Ran

    2016-03-17

    We study the conformal bootstrap for a 4-point function of fermions <ψψψψ> in 3D. We first introduce an embedding formalism for 3D spinors and compute the conformal blocks appearing in fermion 4-point functions. Using these results, we find general bounds on the dimensions of operators appearing in the ψ × ψ OPE, and also on the central charge CT. We observe features in our bounds that coincide with scaling dimensions in the GrossNeveu models at large N. Finally, we also speculate that other features could coincide with a fermionic CFT containing no relevant scalar operators.

  9. 5-Gb/s 0.18-μm CMOS 2:1 multiplexer with integrated clock extraction

    NASA Astrophysics Data System (ADS)

    Changchun, Zhang; Zhigong, Wang; Si, Shi; Peng, Miao; Ling, Tian

    2009-09-01

    A 5-Gb/s 2:1 MUX (multiplexer) with an on-chip integrated clock extraction circuit which possesses the function of automatic phase alignment (APA), has been designed and fabricated in SMIC's 0.18 μm CMOS technology. The chip area is 670 × 780 μm2. At a single supply voltage of 1.8 V, the total power consumption is 112 mW with an input sensitivity of less than 50 mV and an output single-ended swing of above 300 mV. The measurement results show that the IC can work reliably at any input data rate between 1.8 and 2.6 Gb/s with no need for external components, reference clock, or phase alignment between data and clock. It can be used in a parallel optic-fiber data interconnecting system.

  10. Multiplexed Integrating Plasmids for Engineering of the Erythromycin Gene Cluster for Expression in Streptomyces spp. and Combinatorial Biosynthesis

    PubMed Central

    Fayed, Bahgat; Ashford, David A.; Hashem, Amal M.; Amin, Magdy A.; El Gazayerly, Omaima N.; Gregory, Matthew A.

    2015-01-01

    Bacteria in the genus Streptomyces and its close relatives are prolific producers of secondary metabolites with antibiotic activity. Genome sequencing of these bacteria has revealed a rich source of potentially new antibiotic pathways, whose products have never been observed. Moreover, these new pathways can provide novel genes that could be used in combinatorial biosynthesis approaches to generate unnatural analogues of existing antibiotics. We explore here the use of multiple orthologous integrating plasmid systems, based on the int/attP loci from phages TG1, SV1, and ϕBT1, to express the polyketide synthase (PKS) for erythromycin in a heterologous Streptomyces host. Streptomyces strains containing the three polyketide synthase genes eryAI, eryAII, and eryAIII expressed from three different integrated plasmids produced the aglycone intermediate, 6-deoxyerythronolide B (6-dEB). A further pair of integrating plasmids, both derived from the ϕC31 int/attP locus, were constructed carrying a gene cassette for glycosylation of the aglycone intermediates, with or without the tailoring gene, eryF, required for the synthesis of erythronolide B (EB). Liquid chromatography-mass spectrometry of the metabolites indicated the production of angolosaminyl-6-dEB and angolosaminyl-EB. The advantages of using multiplexed integrating plasmids for engineering expression and for combinatorial biosynthesis were demonstrated. PMID:26431970

  11. Venus in 3D

    NASA Technical Reports Server (NTRS)

    Plaut, Jeffrey J.

    1993-01-01

    Stereographic images of the surface of Venus which enable geologists to reconstruct the details of the planet's evolution are discussed. The 120-meter resolution of these 3D images make it possible to construct digital topographic maps from which precise measurements can be made of the heights, depths, slopes, and volumes of geologic structures.

  12. 3D photoacoustic imaging

    NASA Astrophysics Data System (ADS)

    Carson, Jeffrey J. L.; Roumeliotis, Michael; Chaudhary, Govind; Stodilka, Robert Z.; Anastasio, Mark A.

    2010-06-01

    Our group has concentrated on development of a 3D photoacoustic imaging system for biomedical imaging research. The technology employs a sparse parallel detection scheme and specialized reconstruction software to obtain 3D optical images using a single laser pulse. With the technology we have been able to capture 3D movies of translating point targets and rotating line targets. The current limitation of our 3D photoacoustic imaging approach is its inability ability to reconstruct complex objects in the field of view. This is primarily due to the relatively small number of projections used to reconstruct objects. However, in many photoacoustic imaging situations, only a few objects may be present in the field of view and these objects may have very high contrast compared to background. That is, the objects have sparse properties. Therefore, our work had two objectives: (i) to utilize mathematical tools to evaluate 3D photoacoustic imaging performance, and (ii) to test image reconstruction algorithms that prefer sparseness in the reconstructed images. Our approach was to utilize singular value decomposition techniques to study the imaging operator of the system and evaluate the complexity of objects that could potentially be reconstructed. We also compared the performance of two image reconstruction algorithms (algebraic reconstruction and l1-norm techniques) at reconstructing objects of increasing sparseness. We observed that for a 15-element detection scheme, the number of measureable singular vectors representative of the imaging operator was consistent with the demonstrated ability to reconstruct point and line targets in the field of view. We also observed that the l1-norm reconstruction technique, which is known to prefer sparseness in reconstructed images, was superior to the algebraic reconstruction technique. Based on these findings, we concluded (i) that singular value decomposition of the imaging operator provides valuable insight into the capabilities of

  13. Integrated performance of a frequency domain multiplexing readout in the SPT-3G receiver

    NASA Astrophysics Data System (ADS)

    Bender, A. N.; Ade, P. A. R.; Anderson, A. J.; Avva, J.; Ahmed, Z.; Arnold, K.; Austermann, J. E.; Basu Thakur, R.; Benson, B. A.; Bleem, L. E.; Byrum, K.; Carlstrom, J. E.; Carter, F. W.; Chang, C. L.; Cho, H. M.; Cliche, J. F.; Crawford, T. M.; Cukierman, A.; Czaplewski, D. A.; Ding, J.; Divan, R.; de Haan, T.; Dobbs, M. A.; Dutcher, D.; Everett, W.; Gilbert, A.; Groh, J. C.; Guyser, R.; Halverson, N. W.; Harke-Hosemann, A.; Harrington, N. L.; Hattori, K.; Henning, J. W.; Hilton, G. C.; Holzapfel, W. L.; Huang, N.; Irwin, K. D.; Jeong, O.; Khaire, T.; Korman, M.; Kubik, D.; Kuo, C. L.; Lee, A. T.; Leitch, E. M.; Lendinez, S.; Meyer, S. S.; Miller, C. S.; Montgomery, J.; Nadolski, A.; Natoli, T.; Nguyen, H.; Novosad, V.; Padin, S.; Pan, Z.; Pearson, J.; Posada, C. M.; Rahlin, A.; Reichardt, C. L.; Ruhl, J. E.; Saliwanchik, B. R.; Sayre, J. T.; Shariff, J. A.; Shirley, Ian; Shirokoff, E.; Smecher, G.; Sobrin, J.; Stan, L.; Stark, A. A.; Story, K.; Suzuki, A.; Tang, Q. Y.; Thompson, K. L.; Tucker, C.; Vanderlinde, K.; Vieira, J. D.; Wang, G.; Whitehorn, N.; Yefremenko, V.; Yoon, K. W.

    2016-07-01

    The third generation receiver for the South Pole Telescope, SPT-3G, will make extremely deep, arcminuteresolution maps of the temperature and polarization of the cosmic microwave background. The SPT-3G maps will enable studies of the B-mode polarization signature, constraining primordial gravitational waves as well as the effect of massive neutrinos on structure formation in the late universe. The SPT-3G receiver will achieve exceptional sensitivity through a focal plane of 16,000 transition-edge sensor bolometers, an order of magnitude more than the current SPTpol receiver. SPT-3G uses a frequency domain multiplexing (fMux) scheme to read out the focal plane, combining the signals from 64 bolometers onto a single pair of wires. The fMux readout facilitates the large number of detectors in the SPT-3G focal plane by limiting the thermal load due to readout wiring on the 250 millikelvin cryogenic stage. A second advantage of the fMux system is that the operation of each bolometer can be optimized. In addition to these benefits, the fMux readout introduces new challenges into the design and operation of the receiver. The bolometers are operated at a range of frequencies up to 5 MHz, requiring control of stray reactances over a large bandwidth. Additionally, crosstalk between multiplexed detectors will inject large false signals into the data if not adequately mitigated. SPT-3G is scheduled to deploy to the South Pole Telescope in late 2016. Here, we present the pre-deployment performance of the fMux readout system with the SPT-3G focal plane.

  14. 3D-Printed Fluidic Devices for Nanoparticle Preparation and Flow-Injection Amperometry Using Integrated Prussian Blue Nanoparticle-Modified Electrodes.

    PubMed

    Bishop, Gregory W; Satterwhite, Jennifer E; Bhakta, Snehasis; Kadimisetty, Karteek; Gillette, Kelsey M; Chen, Eric; Rusling, James F

    2015-01-01

    A consumer-grade fused filament fabrication (FFF) 3D printer was used to construct fluidic devices for nanoparticle preparation and electrochemical sensing. Devices were printed using poly(ethylene terephthalate) and featured threaded ports to connect polyetheretherketone (PEEK) tubing via printed fittings prepared from acrylonitrile butadiene styrene (ABS). These devices included channels designed to have 800 μm × 800 μm square cross sections and were semitransparent to allow visualization of the solution-filled channels. A 3D-printed device with a Y-shaped mixing channel was used to prepare Prussian blue nanoparticles (PBNPs) under flow rates of 100 to 2000 μL min(-1). PBNPs were then attached to gold electrodes for hydrogen peroxide sensing. 3D-printed devices used for electrochemical measurements featured threaded access ports into which a fitting equipped with reference, counter, and PBNP-modified working electrodes could be inserted. PBNP-modified electrodes enabled amperometric detection of H2O2 in the 3D-printed channel by flow-injection analysis, exhibiting a detection limit of 100 nM and linear response up to 20 μM. These experiments show that a consumer-grade FFF printer can be used to fabricate low-cost fluidic devices for applications similar to those that have been reported with more expensive 3D-printing methods.

  15. Integration of multi-source and multi-scale datasets for 3D structural modeling for subsurface exploration targeting, Luanchuan Mo-polymetallic district, China

    NASA Astrophysics Data System (ADS)

    Wang, Gongwen; Ma, Zhenbo; Li, Ruixi; Song, Yaowu; Qu, Jianan; Zhang, Shouting; Yan, Changhai; Han, Jiangwei

    2017-04-01

    In this paper, multi-source (geophysical, geochemical, geological and remote sensing) datasets were used to construct multi-scale (district-, deposit-, and orebody-scale) 3D geological models and extract 3D exploration criteria for subsurface Mo-polymetallic exploration targeting in the Luanchuan district in China. The results indicate that (i) a series of region-/district-scale NW-trending thrusts controlled main Mo-polymetallic forming, and they were formed by regional Indosinian Qinling orogenic events, the secondary NW-trending district-scale folds and NE-trending faults and the intrusive stock structure are produced based on thrust structure in Caledonian-Indosinian orogenic events; they are ore-bearing zones and ore-forming structures; (ii) the NW-trending district-scale and NE-trending deposit-scale normal faults were crossed and controlled by the Jurassic granite stocks in 3D space, they are associated with the magma-skarn Mo polymetallic mineralization (the 3D buffer distance of ore-forming granite stocks is 600 m) and the NW-trending hydrothermal Pb-Zn deposits which are surrounded by the Jurassic granite stocks and constrained by NW-trending or NE-trending faults (the 3D buffer distance of ore-forming fault is 700 m); and (iii) nine Mo polymetallic and four Pb-Zn targets were identified in the subsurface of the Luanchuan district.

  16. 3D-Printed Fluidic Devices for Nanoparticle Preparation and Flow-Injection Amperometry Using Integrated Prussian Blue Nanoparticle-Modified Electrodes

    PubMed Central

    Bishop, Gregory W.; Satterwhite, Jennifer E.; Bhakta, Snehasis; Kadimisetty, Karteek; Gillette, Kelsey M.; Chen, Eric; Rusling, James F.

    2015-01-01

    A consumer-grade fused filament fabrication (FFF) 3D printer was used to construct fluidic devices for nanoparticle preparation and electrochemical sensing. Devices were printed using poly(ethylene terephthalate) and featured threaded ports to connect polyetheretherketone (PEEK) tubing via printed fittings prepared from acrylonitrile butadiene styrene (ABS). These devices included channels designed to have 800 × 800 µm2 square cross sections and were semitransparent to allow visualization of the solution-filled channels. A 3D-printed device with a Y-shaped mixing channel was used to prepare Prussian blue nanoparticles (PBNPs) under flow rates of 100 to 2000 µL min−1. PBNPs were then attached to gold electrodes for hydrogen peroxide sensing. 3D-printed devices used for electrochemical measurements featured threaded access ports into which a fitting equipped with reference, counter, and PBNP-modified working electrodes could be inserted. PBNP-modified electrodes enabled amperometric detection of H2O2 in the 3D-printed channel by flow-injection analysis, exhibiting a detection limit of 100 nM and linear response up to 20 µM. These experiments show that a consumer-grade FFF printer can be used to fabricate low-cost fluidic devices for applications similar to those that have been reported with more expensive 3D-printing methods. PMID:25901660

  17. Microfluidic multiplexing in bioanalyses.

    PubMed

    Araz, M Kursad; Tentori, Augusto M; Herr, Amy E

    2013-10-01

    The importance of biological assays spans from clinical diagnostics to environmental monitoring. Simultaneous detection of multiple analytes enhances the efficacy of bioassays by providing more data per assay under standardized conditions. Nevertheless, simultaneous handling and assaying of multiple samples, targets, and experimental conditions can be laborious, reagent consuming, and time intensive. Given these demands, microfluidic platforms have emerged over the past two decades as well-suited approaches for multiplexed assays. Microfluidic design supports integration of assay steps and reproducible sample manipulation across large sets of conditions--all relevant to multiplexed assays. Taken together, reduced reagent consumption, faster assay times, and potential for automation stemming from microfluidic assay design are attractive and needed multiplexed assay performance attributes. This review highlights recent advances in multiplexed bioanalyses benefitting from microfluidic integration.

  18. TACO3D. 3-D Finite Element Heat Transfer Code

    SciTech Connect

    Mason, W.E.

    1992-03-04

    TACO3D is a three-dimensional, finite-element program for heat transfer analysis. An extension of the two-dimensional TACO program, it can perform linear and nonlinear analyses and can be used to solve either transient or steady-state problems. The program accepts time-dependent or temperature-dependent material properties, and materials may be isotropic or orthotropic. A variety of time-dependent and temperature-dependent boundary conditions and loadings are available including temperature, flux, convection, and radiation boundary conditions and internal heat generation. Additional specialized features treat enclosure radiation, bulk nodes, and master/slave internal surface conditions (e.g., contact resistance). Data input via a free-field format is provided. A user subprogram feature allows for any type of functional representation of any independent variable. A profile (bandwidth) minimization option is available. The code is limited to implicit time integration for transient solutions. TACO3D has no general mesh generation capability. Rows of evenly-spaced nodes and rows of sequential elements may be generated, but the program relies on separate mesh generators for complex zoning. TACO3D does not have the ability to calculate view factors internally. Graphical representation of data in the form of time history and spatial plots is provided through links to the POSTACO and GRAPE postprocessor codes.

  19. Kinematic Analysis of Fold-Thrust-Belt Using Integrated Analogue Sandbox Modeling and 3D Palinspatic Reconstructions in Babar-Selaru Area, Banda Sea Region, Indonesia

    NASA Astrophysics Data System (ADS)

    Sapiie, Benyamin; Hadiana, Meli; Kurniawan, Ade; Daniel, Dicky; Danio, Harya; Fujimoto, Masamichi; Ohara, Michio; Alam Perdana, Lisnanda; Saputra, Afif

    2016-04-01

    Kinematic analysis of Babar-Selaru fold-thrust-belt is challenging and often difficult particularly in conducting seismic interpretation due to complex structural geometries. Resolving such as issue, in this study we proposed to use integrated seismic interpretation, analogue sandbox modeling and 3D palinspatic reconstructions. This paper is presented results of detail kinematic analysis for understanding tectonic evolution as well as mechanism of fold-thrust-belt in relation to their hydrocarbon prospect. Babar-Selaru Area is located within the collisional boundary between Australian continental margin and Banda Arc region of Indonesia. The area is characterized by complex deformation zone of fold-thrust-belt, involving Mesozoic and Tertiary sedimentary sequences of Australian continental margin. The age of deformation is ranging from 8-5 Ma. Seismic interpretations show two styles of faults developed in the area, which are thrust and normal faults system. The last deformation observed in the Babar Selaru area is controlled by south verging imbricated thin-skinned thrust fault system, with the staircase style of fault detachment. Although, both structural styles occurred in separated locations, they are formed not only in the same time but also related in time and space. Total extension is ranging from 1-3 % where average shortening is in the order of 35-38%. Sandbox modeling is an effective way to study and understand the style, pattern and geometry of the deformed sedimentary sequences in the study area. Based on comparison of five settings experiments (mainly different geological boundary condition) with more than 50 different modeling; deformation is particularly controlled by types and thickness of lithology package and detachment geometry. These two parameters were quite sensitive in generating different deformation style and pattern in Babar-Selaru fold-thrust-belt. Therefore, choosing the right combination of stratigraphy model and material setting are

  20. Test of mode-division multiplexing and demultiplexing in free-space with diffractive transformation optics.

    PubMed

    Ruffato, Gianluca; Massari, Michele; Parisi, Giuseppe; Romanato, Filippo

    2017-04-03

    In recent years, mode-division multiplexing (MDM) has been proposed as a promising solution in order to increase the information capacity of optical networks both in free-space and in optical fiber transmission. Here we present the design, fabrication and test of diffractive optical elements for mode-division multiplexing based on optical transformations in the visible range. Diffractive optics have been fabricated by means of 3D high-resolution electron beam lithography on polymethylmethacrylate resist layer spun over a glass substrate. The same optical sequence was exploited both for input-mode multiplexing and for output-mode sorting after free-space propagation. Their high miniaturization level and efficiency make these optical devices ideal for integration into next-generation platforms for mode-division (de)multiplexing in telecom applications.

  1. BM platform, B Field, Offshore Northwest Java: A case history of multi-disciplinary integration including 3D seismic, reservoir simulation and horizontal drilling

    SciTech Connect

    Cooke, D.; Aziz, A.; Baldauff, J.; Diswarin, N.

    1996-12-31

    This case history describes how a multidisciplinary team used a 3D survey and reservoir simulation to review and revise the development plans for the BM platform, located in the B Field, Offshore Northwest Java, Indonesia. The case history starts with the collection of necessary parophysical, geologic and production data. These data are input to a reservoir simulation which shows there should be no problems with the new platform. However, this initial simulation has known problems with contradicting input structure maps and fluid contacts. Hopefully, these problems can be addressed with a new 3D seismic survey - if the seismic data can be acquired, processed, interpreted and input to the simulation before drilling starts at the BM platform. The seismic acquisition could not be done with the traditional towed seismic streamer cables - instead stationary ocean bottom cables with dual geophone-hydrophone sensors were used. Processing of the seismic data was done in a way that allowed interpretation of the critical area even before acquisition of the entire survey was finished. The new 3D structure maps changed the MDT`s opinion of what reservoir and what areas contained the bulk of the oil reserves. Unfortunately, the new maps were not available until after the jacket was set but before the wells drilled. The NMT updated the simulation with the new 3D data, which led to changes in development well bottom hole locations and an ambitious horizontal well.

  2. Implementation of VITUS 3D Whole Body Scanning for Item Size Selection & Integration to ARN Systems for Issuing at Ft. Jackson

    DTIC Science & Technology

    2004-12-27

    Human Solutions and the management staff at the CIPP to the fitters in the importance of using the recommended sizes. 3.5 Pilot Process – Initial Data...SD The WBX 3D Whole Body Scanner was installed at Marine Corps Recruit Depot during April 2001, replacing the older WB4 model put in service in 1998

  3. RHOCUBE: 3D density distributions modeling code

    NASA Astrophysics Data System (ADS)

    Nikutta, Robert; Agliozzo, Claudia

    2016-11-01

    RHOCUBE models 3D density distributions on a discrete Cartesian grid and their integrated 2D maps. It can be used for a range of applications, including modeling the electron number density in LBV shells and computing the emission measure. The RHOCUBE Python package provides several 3D density distributions, including a powerlaw shell, truncated Gaussian shell, constant-density torus, dual cones, and spiralling helical tubes, and can accept additional distributions. RHOCUBE provides convenient methods for shifts and rotations in 3D, and if necessary, an arbitrary number of density distributions can be combined into the same model cube and the integration ∫ dz performed through the joint density field.

  4. Integration of PCR-Sequencing Analysis with Multiplex Ligation-Dependent Probe Amplification for Diagnosis of Hereditary Fructose Intolerance.

    PubMed

    Ferri, Lorenzo; Caciotti, Anna; Cavicchi, Catia; Rigoldi, Miriam; Parini, Rossella; Caserta, Marina; Chibbaro, Guido; Gasperini, Serena; Procopio, Elena; Donati, Maria Alice; Guerrini, Renzo; Morrone, Amelia

    2012-01-01

    Mutations in the ALDOB gene impair the activity of the hepatic aldolase B enzyme, causing hereditary fructose intolerance (HFI), an inherited autosomic recessive disease of carbohydrate metabolism, that can result in hypoglycemia, liver and kidney failure, coma, and death. Noninvasive diagnosis is possible by identifying mutant ALDOB alleles in suspected patients. We report the genetic characterization of a cohort of 18 HFI Caucasian patients, based on PCR-sequencing and Multiplex Ligation-dependent Probe Amplification (MLPA), with the identification of two novel genetic lesions: a small duplication c.940_941dupT (p.Trp314fsX22) and a large deletion encompassing the promoter region and exon 1. MLPA and long range-PCR (LR-PCR) also identified the recently reported g.7840_14288del6448 allele with a surprisingly high frequency (11%) within our patients' cohort. The most common p.Ala150Pro (44%), p.Ala175Asp (19%), p.Asn335Lys (8%), and/or the known c.360-363del4 (5%), p.Tyr204X (2.8%), IVS6 -2A>G (2.8%) mutant alleles were identified in 14 patients at a homozygous or compound-heterozygous level. The integration of PCR-sequencing analysis with exon-dosage tools [MLPA and quantitative fluorescent multiplex-PCR (QFM-PCR)] led to the full genotyping of patients within our cohort and to the identification of the new deletion encompassing the promoter region and exon 1.

  5. 3D Printed Shelby Cobra

    SciTech Connect

    Love, Lonnie

    2015-01-09

    ORNL's newly printed 3D Shelby Cobra was showcased at the 2015 NAIAS in Detroit. This "laboratory on wheels" uses the Shelby Cobra design, celebrating the 50th anniversary of this model and honoring the first vehicle to be voted a national monument. The Shelby was printed at the Department of Energy’s Manufacturing Demonstration Facility at ORNL using the BAAM (Big Area Additive Manufacturing) machine and is intended as a “plug-n-play” laboratory on wheels. The Shelby will allow research and development of integrated components to be tested and enhanced in real time, improving the use of sustainable, digital manufacturing solutions in the automotive industry.

  6. A geostatistical approach to integrating data from multiple and diverse sources: An application to the integration of well data, geological information, 3d/4d geophysical and reservoir-dynamics data in a north-sea reservoir

    NASA Astrophysics Data System (ADS)

    Caers, Jef; Castro, Scarlet

    Modeling the subsurface is an inherently difficult task due to limited access and lack of direct observation of the complex medium under investigation. Nevertheless, practical engineering questions often call for a full 3D modeling of subsurface heterogeneity, whether the task is to maximize production of an oil reservoir or to optimize storage of water during dry seasons in an aquifer storage and recovery process. While the goal of modeling and the nature of fluid flow may be different between the field of petroleum and hydrogeology, each deals with a similar heterogeneous medium and faces similar questions in model building. Modeling aquifers or reservoirs requires integrating diverse sources of information into a single model (e.g., Deutsch, 2003, Caers, 2005). One faces many challenges in doing so, most related to the issue of scale, since the unit grid cell size of the model is different from the scale of information provided by each source of information. Each such source informs the aquifer or reservoir at a different scale of observation. Secondly, models contain several geological building blocks, such as a structural model (fault/horizons), 3D distribution of facies types, petrophysical properties (porosity and permeability) per facies, fluid distributions and fluid properties, etc.; each building block needs to be constrained to the available data.

  7. Fully Integrated, Multiport, Planar-Waveguide, Spectral Comparators and Multiplexers Based on Lithographic Holography

    DTIC Science & Technology

    2005-08-31

    2005, San Jose, California, USA. 2. C. M. Greiner, T. W. Mossberg, D. Iazikov, "Planar lithographic holography as enabler of integrated photonics (Invited...line set displacement," in Integrated Photonics Research and Applications/Nanophotonicsfor Information Systems Topical Meetings on CD-ROM (The...Based on Holographic Bragg Reflectors," in Integrated Photonics 16 Research and Applications/Nanophotonics for Information Systems Topical Meetings on

  8. Twin Peaks - 3D

    NASA Technical Reports Server (NTRS)

    1997-01-01

    The two hills in the distance, approximately one to two kilometers away, have been dubbed the 'Twin Peaks' and are of great interest to Pathfinder scientists as objects of future study. 3D glasses are necessary to identify surface detail. The white areas on the left hill, called the 'Ski Run' by scientists, may have been formed by hydrologic processes.

    The IMP is a stereo imaging system with color capability provided by 24 selectable filters -- twelve filters per 'eye.

    Click below to see the left and right views individually. [figure removed for brevity, see original site] Left [figure removed for brevity, see original site] Right

  9. 3D and beyond

    NASA Astrophysics Data System (ADS)

    Fung, Y. C.

    1995-05-01

    This conference on physiology and function covers a wide range of subjects, including the vasculature and blood flow, the flow of gas, water, and blood in the lung, the neurological structure and function, the modeling, and the motion and mechanics of organs. Many technologies are discussed. I believe that the list would include a robotic photographer, to hold the optical equipment in a precisely controlled way to obtain the images for the user. Why are 3D images needed? They are to achieve certain objectives through measurements of some objects. For example, in order to improve performance in sports or beauty of a person, we measure the form, dimensions, appearance, and movements.

  10. 3D Audio System

    NASA Technical Reports Server (NTRS)

    1992-01-01

    Ames Research Center research into virtual reality led to the development of the Convolvotron, a high speed digital audio processing system that delivers three-dimensional sound over headphones. It consists of a two-card set designed for use with a personal computer. The Convolvotron's primary application is presentation of 3D audio signals over headphones. Four independent sound sources are filtered with large time-varying filters that compensate for motion. The perceived location of the sound remains constant. Possible applications are in air traffic control towers or airplane cockpits, hearing and perception research and virtual reality development.

  11. Integrated Tsunami Database: simulation and identification of seismic tsunami sources, 3D visualization and post-disaster assessment on the shore

    NASA Astrophysics Data System (ADS)

    Krivorot'ko, Olga; Kabanikhin, Sergey; Marinin, Igor; Karas, Adel; Khidasheli, David

    2013-04-01

    One of the most important problems of tsunami investigation is the problem of seismic tsunami source reconstruction. Non-profit organization WAPMERR (http://wapmerr.org) has provided a historical database of alleged tsunami sources around the world that obtained with the help of information about seaquakes. WAPMERR also has a database of observations of the tsunami waves in coastal areas. The main idea of presentation consists of determining of the tsunami source parameters using seismic data and observations of the tsunami waves on the shore, and the expansion and refinement of the database of presupposed tsunami sources for operative and accurate prediction of hazards and assessment of risks and consequences. Also we present 3D visualization of real-time tsunami wave propagation and loss assessment, characterizing the nature of the building stock in cities at risk, and monitoring by satellite images using modern GIS technology ITRIS (Integrated Tsunami Research and Information System) developed by WAPMERR and Informap Ltd. The special scientific plug-in components are embedded in a specially developed GIS-type graphic shell for easy data retrieval, visualization and processing. The most suitable physical models related to simulation of tsunamis are based on shallow water equations. We consider the initial-boundary value problem in Ω := {(x,y) ?R2 : x ?(0,Lx ), y ?(0,Ly ), Lx,Ly > 0} for the well-known linear shallow water equations in the Cartesian coordinate system in terms of the liquid flow components in dimensional form Here ?(x,y,t) defines the free water surface vertical displacement, i.e. amplitude of a tsunami wave, q(x,y) is the initial amplitude of a tsunami wave. The lateral boundary is assumed to be a non-reflecting boundary of the domain, that is, it allows the free passage of the propagating waves. Assume that the free surface oscillation data at points (xm, ym) are given as a measured output data from tsunami records: fm(t) := ? (xm, ym,t), (xm

  12. Chopped molecular beam multiplexing system

    NASA Technical Reports Server (NTRS)

    Adams, Billy R. (Inventor)

    1986-01-01

    The integration of a chopped molecular beam mass spectrometer with a time multiplexing system is described. The chopping of the molecular beam is synchronized with the time intervals by a phase detector and a synchronous motor. Arithmetic means are generated for phase shifting the chopper with respect to the multiplexer. A four channel amplifier provides the capacity to independently vary the baseline and amplitude in each channel of the multiplexing system.

  13. An integrated system of ABO typing and multiplex STR testing for forensic DNA analysis.

    PubMed

    Jiang, Xianhua; He, Juan; Jia, Fei; Shen, Hongying; Zhao, Jinling; Chen, Chuguang; Bai, Liping; Liu, Feng; Hou, Guangwei; Guo, Faye

    2012-12-01

    A new amplification system for ABO and STR genotyping in a single reaction has been successfully developed. Two types of information can be obtained from a biological sample at one time. One is the classical information of ABO blood group typing for screening suspects and the other is STR information for individual identification. The system allows for the simultaneous detection of 15 autosomal STR loci (containing all CODIS STR loci as well as Penta D and Penta E), six ABO genotypes (O/O, B/B, A/A, A/O, A/B, and B/O) and the gender-determining locus Amelogenin. Primers are designed so that the amplicons are distributed ranging from 75bp to 500bp within a four-dye fluorescent design, leaving a fourth dye for the internal size standard. With 30 cycles, the results showed that the optimal amount of DNA template for this multiplex ranges from 250pg to 2ng and the lowest detection threshold is 125pg (as low as 63pg for ABO loci). For the DNA template outside the optimal detection range, we could adjust the number of cycles to obtain the robust profiles. Mixture studies showed that over 83% of minor alleles were detected at 1:9 ratios. The full profiles were still observed when 4ng of degraded DNA was digested by DNase I and 1ng undegraded DNA was added to 40μM haematin. Polymerase chain reaction (PCR)-based conditions including the concentrations of primers, magnesium and the Taq polymerase as well as volume, cycle numbers and annealing temperature were examined and optimised. In addition, the system was validated by 364 bloodstain samples and 32 common casework samples. According to the Chinese National Standards and Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines, our system demonstrates good detection performance and is an ideal tool for forensic DNA typing with potential application.

  14. Workflow for the integration of a realistic 3D geomodel in process simulations using different cell types and advanced scientific visualization: Variations on a synthetic salt diapir

    NASA Astrophysics Data System (ADS)

    Görz, Ines; Herbst, Martin; Börner, Jana H.; Zehner, Björn

    2017-03-01

    The purpose of this study is to use one complex geological 3D model for numerical simulations of various physical processes in process-specific simulation software. To do this, the 3D model has to be discretized according to different cell types, depending on the requirements of the simulation method. We used a salt structure with a diapir and its deformed host rock to produce two 3D models describing the boundary surfaces of the structure: one very simplified model consisting of cuboid surfaces and a realistic model consisting of irregular boundary surfaces. We provide a workflow for how to generate hexahedral, tetrahedral and spherical volume representations of these two geometries. We utilized the volume representations to simulate temperature, displacement and transient electromagnetic fields. We can show that the simulation results closely reflect the input geometry and that it is worth the effort to produce geometric models that are as realistic as possible. Additionally, we provide a workflow for simultaneous visualization and analysis of the simulation results. Scientific visualization is an important tool for deriving knowledge from complex investigations.

  15. Optical full-depth refocusing of 3-D objects based on subdivided-elemental images and local periodic δ-functions in integral imaging.

    PubMed

    Ai, Ling-Yu; Dong, Xiao-Bin; Jang, Jae-Young; Kim, Eun-Soo

    2016-05-16

    We propose a new approach for optical refocusing of three-dimensional (3-D) objects on their real depth without a pickup-range limitation based on subdivided-elemental image arrays (sub-EIAs) and local periodic δ-function arrays (L-PDFAs). The captured EIA from the 3-D objects locating out of the pickup-range, is divided into a number of sub-EIAs depending on the object distance from the lens array. Then, by convolving these sub-EIAs with each L-PDFA whose spatial period corresponds to the specific object's depth, as well as whose size is matched to that of the sub-EIA, arrays of spatially-filtered sub-EIAs (SF-sub-EIAs) for each object depth can be uniquely extracted. From these arrays of SF-sub-EIAs, 3-D objects can be optically reconstructed to be refocused on their real depth. Operational principle of the proposed method is analyzed based on ray-optics. In addition, to confirm the feasibility of the proposed method in the practical application, experiments with test objects are carried out and the results are comparatively discussed with those of the conventional method.

  16. 3D Surgical Simulation

    PubMed Central

    Cevidanes, Lucia; Tucker, Scott; Styner, Martin; Kim, Hyungmin; Chapuis, Jonas; Reyes, Mauricio; Proffit, William; Turvey, Timothy; Jaskolka, Michael

    2009-01-01

    This paper discusses the development of methods for computer-aided jaw surgery. Computer-aided jaw surgery allows us to incorporate the high level of precision necessary for transferring virtual plans into the operating room. We also present a complete computer-aided surgery (CAS) system developed in close collaboration with surgeons. Surgery planning and simulation include construction of 3D surface models from Cone-beam CT (CBCT), dynamic cephalometry, semi-automatic mirroring, interactive cutting of bone and bony segment repositioning. A virtual setup can be used to manufacture positioning splints for intra-operative guidance. The system provides further intra-operative assistance with the help of a computer display showing jaw positions and 3D positioning guides updated in real-time during the surgical procedure. The CAS system aids in dealing with complex cases with benefits for the patient, with surgical practice, and for orthodontic finishing. Advanced software tools for diagnosis and treatment planning allow preparation of detailed operative plans, osteotomy repositioning, bone reconstructions, surgical resident training and assessing the difficulties of the surgical procedures prior to the surgery. CAS has the potential to make the elaboration of the surgical plan a more flexible process, increase the level of detail and accuracy of the plan, yield higher operative precision and control, and enhance documentation of cases. Supported by NIDCR DE017727, and DE018962 PMID:20816308

  17. Martian terrain - 3D

    NASA Technical Reports Server (NTRS)

    1997-01-01

    An area of rocky terrain near the landing site of the Sagan Memorial Station can be seen in this image, taken in stereo by the Imager for Mars Pathfinder (IMP) on Sol 3. 3D glasses are necessary to identify surface detail. This image is part of a 3D 'monster' panorama of the area surrounding the landing site.

    Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

    Click below to see the left and right views individually. [figure removed for brevity, see original site] Left [figure removed for brevity, see original site] Right

  18. Pseudo single crystal, direct-band-gap Ge{sub 0.89}Sn{sub 0.11} on amorphous dielectric layers towards monolithic 3D photonic integration

    SciTech Connect

    Li, Haofeng; Brouillet, Jeremy; Wang, Xiaoxin; Liu, Jifeng

    2014-11-17

    We demonstrate pseudo single crystal, direct-band-gap Ge{sub 0.89}Sn{sub 0.11} crystallized on amorphous layers at <450 °C towards 3D Si photonic integration. We developed two approaches to seed the lateral single crystal growth: (1) utilize the Gibbs-Thomson eutectic temperature depression at the tip of an amorphous GeSn nanotaper for selective nucleation; (2) laser-induced nucleation at one end of a GeSn strip. Either way, the crystallized Ge{sub 0.89}Sn{sub 0.11} is dominated by a single grain >18 μm long that forms optoelectronically benign twin boundaries with others grains. These pseudo single crystal, direct-band-gap Ge{sub 0.89}Sn{sub 0.11} patterns are suitable for monolithic 3D integration of active photonic devices on Si.

  19. 3D field harmonics

    SciTech Connect

    Caspi, S.; Helm, M.; Laslett, L.J.

    1991-03-30

    We have developed an harmonic representation for the three dimensional field components within the windings of accelerator magnets. The form by which the field is presented is suitable for interfacing with other codes that make use of the 3D field components (particle tracking and stability). The field components can be calculated with high precision and reduced cup time at any location (r,{theta},z) inside the magnet bore. The same conductor geometry which is used to simulate line currents is also used in CAD with modifications more readily available. It is our hope that the format used here for magnetic fields can be used not only as a means of delivering fields but also as a way by which beam dynamics can suggest correction to the conductor geometry. 5 refs., 70 figs.

  20. Integration of 2D and 3D reflection seismic data with deep boreholes in the Kevitsa Ni-Cu-PGE deposit, northern Finland

    NASA Astrophysics Data System (ADS)

    Koivisto, Emilia; Malehmir, Alireza; Voipio, Teemu; Wijns, Chris

    2013-04-01

    Kevitsa is a large disseminated sulphide Ni-Cu-PGE deposit hosted by the Kevitsa mafic-ultramafic intrusion in northern Finland and dated as about 2.06 Ga old. The Geological Survey of Finland first discovered the Kevitsa deposit in 1987. Open pit mining by Kevitsa Mining Oy/First Quantum Minerals Ltd. commenced in June 2012. The final pit depth is planned to be 550-600 m. The estimated ore reserves of the Kevitsa intrusion are about 240 million tones (using a nickel cut-off grade of 0.1%). The expected life-of-mine is 20-30 years. More than 400 hundred holes have been drilled in the Kevitsa area, but most are concentrated close to the known deposit and do not provide a comprehensive understanding of the extent of the intrusion. The basal contact of the intrusion is penetrated by only about 30 drill holes, most of which are shallow. A better knowledge of the geometry of the intrusion would provide a framework for near-mine and deep exploration in the area. An exact knowledge on the basal contact of the intrusion would also provide an exploration target for the contact-type mineralization that is often more massive and richer in Ni-Cu. In December 2007, a series of 2D reflection seismic profiles was acquired in the Kevitsa area. It consisted of four connected survey lines between 6 and 11 km long. In 2010, the initial positive results of the 2D seismic survey led Kevitsa Mining Oy/First Quantum Minerals Ltd. to initiate a 3D reflection seismic survey. The 3D seismic survey is limited to the closer vicinity of the known deposit, while the 2D seismic survey was designed to provide a more regional view of the Kevitsa intrusive complex. The main aims of the 2D and 3D seismic surveys were to delineate the shape and extent of the ore-bearing Kevitsa intrusion and the geometry of some of the host rock and surrounding units, and extract information about the larger-scale structures and structures important for mine-planning purposes. The 2D and 3D seismic data were used to

  1. Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells.

    PubMed

    Nissim, Lior; Perli, Samuel D; Fridkin, Alexandra; Perez-Pinera, Pablo; Lu, Timothy K

    2014-05-22

    RNA-based regulation and CRISPR/Cas transcription factors (CRISPR-TFs) have the potential to be integrated for the tunable modulation of gene networks. A major limitation of this methodology is that guide RNAs (gRNAs) for CRISPR-TFs can only be expressed from RNA polymerase III promoters in human cells, limiting their use for conditional gene regulation. We present new strategies that enable expression of functional gRNAs from RNA polymerase II promoters and multiplexed production of proteins and gRNAs from a single transcript in human cells. We use multiple RNA regulatory strategies, including RNA-triple-helix structures, introns, microRNAs, and ribozymes, with Cas9-based CRISPR-TFs and Cas6/Csy4-based RNA processing. Using these tools, we efficiently modulate endogenous promoters and implement tunable synthetic circuits, including multistage cascades and RNA-dependent networks that can be rewired with Csy4 to achieve complex behaviors. This toolkit can be used for programming scalable gene circuits and perturbing endogenous networks for biology, therapeutic, and synthetic biology applications.

  2. An integrated chip for rapid, sensitive, and multiplexed detection of cardiac biomarkers from fingerprick blood.

    PubMed

    Zhang, Guo-Jun; Luo, Zhan Hong Henry; Huang, Min Joon; Ang, Jun'an Jason; Kang, Tae Goo; Ji, Hongmiao

    2011-10-15

    Cardiovascular diseases are the major cause of death among adults worldwide. Electrocardiogram (ECG) is a first test when a patient suffering from chest pain sees a doctor, however, it is lack of the required sensitivity. Standard assays to detect cardiac biomarkers, like enzyme-linked immunosorbent assay (ELISA) are sensitive, but suffer from important sample and reagent consumption in large-scale studies. Moreover they are performed in central laboratories of clinics and hospitals and take a long time, which is highly incompatible with the quick decisions needed to save a heart attack patient. Herein, we describe an integrated chip allowing rapid, sensitive, and simultaneous analysis of three cardiac biomarkers in fingerprick blood. The integrated chip is composed of a filtration chip for plasma separation from blood and a silicon nanowire (SiNW) array sensor chip for protein detection. These two chips are fabricated separately and bonded to form a single unit after alignment. The integrated chip is capable of reducing the dead volume of the sample by eliminating the tubing between the two chips. After the plasma is filtrated by the filtration chip, the SiNW sensor, spotted with three different antibodies, enabled us to detect three cardiac biomarkers, troponin T (cTnT), creatine kinase MM (CK-MM) and creatine kinase MB (CK-MB), simultaneously. The integrated chip is able to attain a low detection limit of 1 pg/ml for the three cardiac biomarkers from 2 μl blood in 45 min.

  3. Interactive 3D Mars Visualization

    NASA Technical Reports Server (NTRS)

    Powell, Mark W.

    2012-01-01

    The Interactive 3D Mars Visualization system provides high-performance, immersive visualization of satellite and surface vehicle imagery of Mars. The software can be used in mission operations to provide the most accurate position information for the Mars rovers to date. When integrated into the mission data pipeline, this system allows mission planners to view the location of the rover on Mars to 0.01-meter accuracy with respect to satellite imagery, with dynamic updates to incorporate the latest position information. Given this information so early in the planning process, rover drivers are able to plan more accurate drive activities for the rover than ever before, increasing the execution of science activities significantly. Scientifically, this 3D mapping information puts all of the science analyses to date into geologic context on a daily basis instead of weeks or months, as was the norm prior to this contribution. This allows the science planners to judge the efficacy of their previously executed science observations much more efficiently, and achieve greater science return as a result. The Interactive 3D Mars surface view is a Mars terrain browsing software interface that encompasses the entire region of exploration for a Mars surface exploration mission. The view is interactive, allowing the user to pan in any direction by clicking and dragging, or to zoom in or out by scrolling the mouse or touchpad. This set currently includes tools for selecting a point of interest, and a ruler tool for displaying the distance between and positions of two points of interest. The mapping information can be harvested and shared through ubiquitous online mapping tools like Google Mars, NASA WorldWind, and Worldwide Telescope.

  4. From Dynamic Live Cell Imaging to 3D Ultrastructure: Novel Integrated Methods for High Pressure Freezing and Correlative Light-Electron Microscopy

    PubMed Central

    Spiegelhalter, Coralie; Tosch, Valérie; Hentsch, Didier; Koch, Marc; Kessler, Pascal; Schwab, Yannick; Laporte, Jocelyn

    2010-01-01

    Background In cell biology, the study of proteins and organelles requires the combination of different imaging approaches, from live recordings with light microscopy (LM) to electron microscopy (EM). Methodology To correlate dynamic events in adherent cells with both ultrastructural and 3D information, we developed a method for cultured cells that combines confocal time-lapse images of GFP-tagged proteins with electron microscopy. With laser micro-patterned culture substrate, we created coordinates that were conserved at every step of the sample preparation and visualization processes. Specifically designed for cryo-fixation, this method allowed a fast freezing of dynamic events within seconds and their ultrastructural characterization. We provide examples of the dynamic oligomerization of GFP-tagged myotubularin (MTM1) phosphoinositides phosphatase induced by osmotic stress, and of the ultrastructure of membrane tubules dependent on amphiphysin 2 (BIN1) expression. Conclusion Accessible and versatile, we show that this approach is efficient to routinely correlate functional and dynamic LM with high resolution morphology by EM, with immuno-EM labeling, with 3D reconstruction using serial immuno-EM or tomography, and with scanning-EM. PMID:20140253

  5. On the integration of protein-protein interaction networks with gene expression and 3D structural data: What can be gained?

    NASA Astrophysics Data System (ADS)

    Bertolazzi, Paola; Bock, Mary Ellen; Guerra, Concettina; Paci, Paola; Santoni, Daniele

    2014-06-01

    The biological role of proteins has been analyzed from different perspectives, initially by considering proteins as isolated biological entities, then as cooperating entities that perform their function by interacting with other molecules. There are other dimensions that are important for the complete understanding of the biological processes: time and location. However a protein is rarely annotated with temporal and spatial information. Experimental Protein-Proteins Interaction (PPI) data are static; furthermore they generally do not include transient interactions which are a considerable fraction of the interactome of many organisms. One way to incorporate temporal and condition information is to use other sources of information, such as gene expression data and 3D structural data. Here we review work done to understand the insight that can be gained by enriching PPI data with gene expression and 3D structural data. In particular, we address the following questions: Can the dynamics of a single protein or of an interaction be accurately derived from these data? Can the assembly-disassembly of protein complexes be traced over time? What type of topological changes occur in a PPI network architecture over time?

  6. 3D printed bionic ears.

    PubMed

    Mannoor, Manu S; Jiang, Ziwen; James, Teena; Kong, Yong Lin; Malatesta, Karen A; Soboyejo, Winston O; Verma, Naveen; Gracias, David H; McAlpine, Michael C

    2013-06-12

    The ability to three-dimensionally interweave biological tissue with functional electronics could enable the creation of bionic organs possessing enhanced functionalities over their human counterparts. Conventional electronic devices are inherently two-dimensional, preventing seamless multidimensional integration with synthetic biology, as the processes and materials are very different. Here, we present a novel strategy for overcoming these difficulties via additive manufacturing of biological cells with structural and nanoparticle derived electronic elements. As a proof of concept, we generated a bionic ear via 3D printing of a cell-seeded hydrogel matrix in the anatomic geometry of a human ear, along with an intertwined conducting polymer consisting of infused silver nanoparticles. This allowed for in vitro culturing of cartilage tissue around an inductive coil antenna in the ear, which subsequently enables readout of inductively-coupled signals from cochlea-shaped electrodes. The printed ear exhibits enhanced auditory sensing for radio frequency reception, and complementary left and right ears can listen to stereo audio music. Overall, our approach suggests a means to intricately merge biologic and nanoelectronic functionalities via 3D printing.

  7. 3D Printed Bionic Ears

    PubMed Central

    Mannoor, Manu S.; Jiang, Ziwen; James, Teena; Kong, Yong Lin; Malatesta, Karen A.; Soboyejo, Winston O.; Verma, Naveen; Gracias, David H.; McAlpine, Michael C.

    2013-01-01

    The ability to three-dimensionally interweave biological tissue with functional electronics could enable the creation of bionic organs possessing enhanced functionalities over their human counterparts. Conventional electronic devices are inherently two-dimensional, preventing seamless multidimensional integration with synthetic biology, as the processes and materials are very different. Here, we present a novel strategy for overcoming these difficulties via additive manufacturing of biological cells with structural and nanoparticle derived electronic elements. As a proof of concept, we generated a bionic ear via 3D printing of a cell-seeded hydrogel matrix in the precise anatomic geometry of a human ear, along with an intertwined conducting polymer consisting of infused silver nanoparticles. This allowed for in vitro culturing of cartilage tissue around an inductive coil antenna in the ear, which subsequently enables readout of inductively-coupled signals from cochlea-shaped electrodes. The printed ear exhibits enhanced auditory sensing for radio frequency reception, and complementary left and right ears can listen to stereo audio music. Overall, our approach suggests a means to intricately merge biologic and nanoelectronic functionalities via 3D printing. PMID:23635097

  8. Multiplexing electro-optic architectures for advanced aircraft integrated flight control systems

    NASA Technical Reports Server (NTRS)

    Seal, D. W.

    1989-01-01

    This report describes the results of a 10 month program sponsored by NASA. The objective of this program was to evaluate various optical sensor modulation technologies and to design an optimal Electro-Optic Architecture (EOA) for servicing remote clusters of sensors and actuators in advanced aircraft flight control systems. The EOA's supply optical power to remote sensors and actuators, process the modulated optical signals returned from the sensors, and produce conditioned electrical signals acceptable for use by a digital flight control computer or Vehicle Management System (VMS) computer. This study was part of a multi-year initiative under the Fiber Optic Control System Integration (FOCSI) program to design, develop, and test a totally integrated fiber optic flight/propulsion control system for application to advanced aircraft. Unlike earlier FOCSI studies, this program concentrated on the design of the EOA interface rather than the optical transducer technology itself.

  9. Ultra-thin silicon (UTSi) on insulator CMOS transceiver and time-division multiplexed switch chips for smart pixel integration

    NASA Astrophysics Data System (ADS)

    Zhang, Liping; Sawchuk, Alexander A.

    2001-12-01

    We describe the design, fabrication and functionality of two different 0.5 micron CMOS optoelectronic integrated circuit (OEIC) chips based on the Peregrine Semiconductor Ultra-Thin Silicon on insulator technology. The Peregrine UTSi silicon- on-sapphire (SOS) technology is a member of the silicon-on- insulator (SOI) family. The low-loss synthetic sapphire substrate is optically transparent and has good thermal conductivity and coefficient of thermal expansion properties, which meet the requirements for flip-chip bonding of VCSELs and other optoelectronic input-output components. One chip contains transceiver and network components, including four channel high-speed CMOS transceiver modules, pseudo-random bit stream (PRBS) generators, a voltage controlled oscillator (VCO) and other test circuits. The transceiver chips can operate in both self-testing mode and networking mode. An on- chip clock and true-single-phase-clock (TSPC) D-flip-flop have been designed to generate a PRBS at over 2.5 Gb/s for the high-speed transceiver arrays to operate in self-testing mode. In the networking mode, an even number of transceiver chips forms a ring network through free-space or fiber ribbon interconnections. The second chip contains four channel optical time-division multiplex (TDM) switches, optical transceiver arrays, an active pixel detector and additional test devices. The eventual applications of these chips will require monolithic OEICs with integrated optical input and output. After fabrication and testing, the CMOS transceiver array dies will be packaged with 850 nm vertical cavity surface emitting lasers (VCSELs), and metal-semiconductor- metal (MSM) or GaAs p-i-n detector die arrays to achieve high- speed optical interconnections. The hybrid technique could be either wire bonding or flip-chip bonding of the CMOS SOS smart-pixel arrays with arrays of VCSELs and photodetectors onto an optoelectronic chip carrier as a multi-chip module (MCM).

  10. Development of frequency domain multiplexing for the X-ray Integral Field unit (X-IFU) on the Athena

    NASA Astrophysics Data System (ADS)

    Akamatsu, Hiroki; Gottardi, Luciano; van der Kuur, Jan; de Vries, Cor P.; Ravensberg, Kevin; Adams, Joseph S.; Bandler, Simon R.; Bruijn, Marcel P.; Chervenak, James A.; Kilbourne, Caroline A.; Kiviranta, Mikko; van der Linden, A. J.; Jackson, Brian D.; Smith, Stephen J.

    2016-07-01

    We are developing the frequency domain multiplexing (FDM) read-out of transition-edge sensor (TES) microcalorimeters for the X-ray Integral Field Unit (X-IFU) instrument on board of the future European X-Ray observatory Athena. The X-IFU instrument consists of an array of 3840 TESs with a high quantum efficiency (>90 %) and spectral resolution ΔE=2.5 eV @ 7 keV (E/ ΔE 2800). FDM is currently the baseline readout system for the X-IFU instrument. Using high quality factor LC filters and room temperature electronics developed at SRON and low-noise two stage SQUID amplifiers provided by VTT, we have recently demonstrated good performance with the FDM readout of Mo/Au TES calorimeters with Au/Bi absorbers. An integrated noise equivalent power resolution of about 2.0 eV at 1.7 MHz has been demonstrated with a pixel from a new TES array from NASA/Goddard (GSFC-A2). We have achieved X-ray energy resolutions 2.5 eV at AC bias frequency at 1.7 MHz in the single pixel read-out. We have also demonstrated for the first time an X-ray energy resolution around 3.0 eV in a 6 pixel FDM read-out with TES array (GSFC-A1). In this paper we report on the single pixel performance of these microcalorimeters under MHz AC bias, and further results of the performance of these pixels under FDM.

  11. Intraoral 3D scanner

    NASA Astrophysics Data System (ADS)

    Kühmstedt, Peter; Bräuer-Burchardt, Christian; Munkelt, Christoph; Heinze, Matthias; Palme, Martin; Schmidt, Ingo; Hintersehr, Josef; Notni, Gunther

    2007-09-01

    Here a new set-up of a 3D-scanning system for CAD/CAM in dental industry is proposed. The system is designed for direct scanning of the dental preparations within the mouth. The measuring process is based on phase correlation technique in combination with fast fringe projection in a stereo arrangement. The novelty in the approach is characterized by the following features: A phase correlation between the phase values of the images of two cameras is used for the co-ordinate calculation. This works contrary to the usage of only phase values (phasogrammetry) or classical triangulation (phase values and camera image co-ordinate values) for the determination of the co-ordinates. The main advantage of the method is that the absolute value of the phase at each point does not directly determine the coordinate. Thus errors in the determination of the co-ordinates are prevented. Furthermore, using the epipolar geometry of the stereo-like arrangement the phase unwrapping problem of fringe analysis can be solved. The endoscope like measurement system contains one projection and two camera channels for illumination and observation of the object, respectively. The new system has a measurement field of nearly 25mm × 15mm. The user can measure two or three teeth at one time. So the system can by used for scanning of single tooth up to bridges preparations. In the paper the first realization of the intraoral scanner is described.

  12. 'Diamond' in 3-D

    NASA Technical Reports Server (NTRS)

    2004-01-01

    This 3-D, microscopic imager mosaic of a target area on a rock called 'Diamond Jenness' was taken after NASA's Mars Exploration Rover Opportunity ground into the surface with its rock abrasion tool for a second time.

    Opportunity has bored nearly a dozen holes into the inner walls of 'Endurance Crater.' On sols 177 and 178 (July 23 and July 24, 2004), the rover worked double-duty on Diamond Jenness. Surface debris and the bumpy shape of the rock resulted in a shallow and irregular hole, only about 2 millimeters (0.08 inch) deep. The final depth was not enough to remove all the bumps and leave a neat hole with a smooth floor. This extremely shallow depression was then examined by the rover's alpha particle X-ray spectrometer.

    On Sol 178, Opportunity's 'robotic rodent' dined on Diamond Jenness once again, grinding almost an additional 5 millimeters (about 0.2 inch). The rover then applied its Moessbauer spectrometer to the deepened hole. This double dose of Diamond Jenness enabled the science team to examine the rock at varying layers. Results from those grindings are currently being analyzed.

    The image mosaic is about 6 centimeters (2.4 inches) across.

  13. Prominent rocks - 3D

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Many prominent rocks near the Sagan Memorial Station are featured in this image, taken in stereo by the Imager for Mars Pathfinder (IMP) on Sol 3. 3D glasses are necessary to identify surface detail. Wedge is at lower left; Shark, Half-Dome, and Pumpkin are at center. Flat Top, about four inches high, is at lower right. The horizon in the distance is one to two kilometers away.

    Mars Pathfinder is the second in NASA's Discovery program of low-cost spacecraft with highly focused science goals. The Jet Propulsion Laboratory, Pasadena, CA, developed and manages the Mars Pathfinder mission for NASA's Office of Space Science, Washington, D.C. JPL is an operating division of the California Institute of Technology (Caltech). The Imager for Mars Pathfinder (IMP) was developed by the University of Arizona Lunar and Planetary Laboratory under contract to JPL. Peter Smith is the Principal Investigator.

    Click below to see the left and right views individually. [figure removed for brevity, see original site] Left [figure removed for brevity, see original site] Right

  14. 3D Visualization Development of SIUE Campus

    NASA Astrophysics Data System (ADS)

    Nellutla, Shravya

    Geographic Information Systems (GIS) has progressed from the traditional map-making to the modern technology where the information can be created, edited, managed and analyzed. Like any other models, maps are simplified representations of real world. Hence visualization plays an essential role in the applications of GIS. The use of sophisticated visualization tools and methods, especially three dimensional (3D) modeling, has been rising considerably due to the advancement of technology. There are currently many off-the-shelf technologies available in the market to build 3D GIS models. One of the objectives of this research was to examine the available ArcGIS and its extensions for 3D modeling and visualization and use them to depict a real world scenario. Furthermore, with the advent of the web, a platform for accessing and sharing spatial information on the Internet, it is possible to generate interactive online maps. Integrating Internet capacity with GIS functionality redefines the process of sharing and processing the spatial information. Enabling a 3D map online requires off-the-shelf GIS software, 3D model builders, web server, web applications and client server technologies. Such environments are either complicated or expensive because of the amount of hardware and software involved. Therefore, the second objective of this research was to investigate and develop simpler yet cost-effective 3D modeling approach that uses available ArcGIS suite products and the free 3D computer graphics software for designing 3D world scenes. Both ArcGIS Explorer and ArcGIS Online will be used to demonstrate the way of sharing and distributing 3D geographic information on the Internet. A case study of the development of 3D campus for the Southern Illinois University Edwardsville is demonstrated.

  15. 3-D treatment planning and dose delivery verification integrating a variety of state-of-the-art techniques: a case report.

    PubMed

    Kuchnir, F T; Watson-Bullock, S; Reft, C S; Hallahan, D

    1991-12-01

    A patient previously treated with radiation for base-of-tongue cancer presented with recurrent disease seven years later. The spinal cord had received tolerance dose. Using state-of-the-art treatment planning techniques, including beam's-eye-view and volumetrics, dose-volume histograms, split field technique, mixed energies, and beam intensity modulation (with a compensator), we achieved uniform dose coverage of the target in 3-D. This was verified in vivo with thermoluminescence dosimeters positioned in the esophagus by means of a nasogastric tube that ran centrally through the target volume. The various techniques applied will be presented with a discussion of the rationale used in each step of plan optimization and verification.

  16. 3D modelling of the active normal fault network in the Apulian Ridge (Eastern Mediterranean Sea): Integration of seismic and bathymetric data with implicit surface methods

    NASA Astrophysics Data System (ADS)

    Bistacchi, Andrea; Pellegrini, Caludio; Savini, Alessandra; Marchese, Fabio

    2016-04-01

    The Apulian ridge (North-eastern Ionian Sea, Mediterranean), interposed between the facing Apennines and Hellenides subduction zones (to the west and east respectively), is characterized by thick cretaceous carbonatic sequences and discontinuous tertiary deposits crosscut by a penetrative network of NNW-SSE normal faults. These are exposed onshore in Puglia, and are well represented offshore in a dataset composed of 2D seismics and wells collected by oil companies from the '60s to the '80s, more recent seismics collected during research projects in the '90s, recent very high resolution seismics (VHRS - Sparker and Chirp-sonar data), multibeam echosounder bathymetry, and sedimentological and geo-chronological analyses of sediment samples collected on the seabed. Faults are evident in 2D seismics at all scales, and their along-strike geometry and continuity can be characterized with multibeam bathymetric data, which show continuous fault scarps on the seabed (only partly reworked by currents and covered by landslides). Fault scarps also reveal the finite displacement accumulated in the Holocene-Pleistocene. We reconstructed a 3D model of the fault network and suitable geological boundaries (mainly unconformities due to the discontinuous distribution of quaternary and tertiary sediments) with implicit surface methods implemented in SKUA/GOCAD. This approach can be considered very effective and allowed reconstructing in details complex structures, like the frequent relay zones that are particularly well imaged by seafloor geomorphology. Mutual cross-cutting relationships have been recognized between fault scarps and submarine mass-wasting deposits (Holocene-Pleistocene), indicating that, at least in places, these features are coeval, hence the fault network should be considered active. At the regional scale, the 3D model allowed measuring the horizontal WSW-ENE stretching, which can be associated to the bending moment applied to the Apulian Plate by the combined effect

  17. 3D Printed Shelby Cobra

    ScienceCinema

    Love, Lonnie

    2016-11-02

    ORNL's newly printed 3D Shelby Cobra was showcased at the 2015 NAIAS in Detroit. This "laboratory on wheels" uses the Shelby Cobra design, celebrating the 50th anniversary of this model and honoring the first vehicle to be voted a national monument. The Shelby was printed at the Department of Energy’s Manufacturing Demonstration Facility at ORNL using the BAAM (Big Area Additive Manufacturing) machine and is intended as a “plug-n-play” laboratory on wheels. The Shelby will allow research and development of integrated components to be tested and enhanced in real time, improving the use of sustainable, digital manufacturing solutions in the automotive industry.

  18. DC and RF characterization of InGaAs replacement metal gate (RMG) nFETs on SiGe-OI FinFETs fabricated by 3D monolithic integration

    NASA Astrophysics Data System (ADS)

    Deshpande, V.; Djara, V.; O'Connor, E.; Hashemi, P.; Balakrishnan, K.; Caimi, D.; Sousa, M.; Czornomaz, L.; Fompeyrine, J.

    2017-02-01

    We report the first RF characterization of short-channel replacement metal gate (RMG) InGaAs-OI nFETs built in a 3D monolithic (3DM) CMOS process. This process features RMG InGaAs-OI nFET top layer and SiGe-OI fin pFET bottom layer. We demonstrate state-of-the-art device integration on both levels. The bottom layer SiGe-OI pFETs are fabricated with a Gate-First (GF) process with fins and featuring epitaxial raised source drain (RSD) as well as silicide contact layer. The top layer InGaAs nFETs are fabricated with a RMG process featuring a self-aligned epitaxial raised source drain (RSD). We show that the 3D monolithic integration scheme does not degrade the performance of the bottom SiGe-OI pFETs owing to an optimized thermal budget for the top InGaAs nFETs. From the RF characterizations performed (post-3D monolithic process) on multifinger-gate InGaAs-OI nFETs, we extract a cut-off frequency (Ft) of 16.4 GHz at a gate-length (Lg) of 120 nm. Measurements on various gate lengths shows increasing cut-off frequency with decreasing gate-length.

  19. Integrated Particle Handling Methods for Multiplexed Microbial Identification and Characterization in Sediments and Groundwater

    SciTech Connect

    Darrell P. Chandler; Ann E. Jarrell; Craig S. Criddle

    2004-03-17

    Molecular methods are still relatively ineffectual for monitoring community dynamics during bioremediation, due primarily to the cost, technical difficulty and retrospective nature of the analyses. For nucleic acid analyses to meaningfully contribute to bioremediation efforts they must not only contribute to the fundamental understanding of microbial ecology, but also be formatted in such a manner that infield analysis can be achieved. The objective of this project is therefore to develop an integrated microbial and nucleic acid detection method and prototype system for the characterization and analysis of subsurface sediments, focusing on the molecular detection of metal and sulfate-reducing bacteria and activity in sediments obtained from the Oak Ridge Field Research Center. We are meeting this objective by combining environmental molecular microbiology with renewable surface techniques, microfluidic systems and microparticle analytical chemistry. The fluidic systems are used to evaluate hypotheses on the integrated biochemistry that is necessary to directly detect 16S rRNA from metal-reducing microbial communities on a suspension microarray, without using the polymerase chain reaction (PCR). These investigations include the use of peptide nucleic acid capture and detection probes and 'tunable surface' concepts to increase nucleic acid capture and detection efficiency and/or mitigate interferences due to co-extracted humic acids. The unified microparticle sample preparation method and suspension array is then used to characterize the 16S rRNA metal- and sulfate-reducing microbial community in FRC sediments before and after biostimulation.

  20. A simple integrated microfluidic device for the multiplexed fluorescence-free detection of Salmonella enterica.

    PubMed

    Strachan, Briony C; Sloane, Hillary S; Houpt, Eric; Lee, Jacob C; Miranian, Daniel C; Li, Jingyi; Nelson, Daniel A; Landers, James P

    2016-02-07

    Rapid, inexpensive and simplistic nucleic acid testing (NAT) is pivotal in delivering biotechnology solutions at the point-of-care (POC). We present a poly(methylmethacrylate) (PMMA) microdevice where on-board infrared-mediated PCR amplification is seamlessly integrated with a particle-based, visual DNA detection for specific detection of bacterial targets in less than 35 minutes. Fluidic control is achieved using a capillary burst valve laser-ablated in a novel manner to confine the PCR reagents to a chamber during thermal cycling, and a manual torque-actuated pressure system to mobilize the fluid from the PCR chamber to the detection reservoir containing oligonucleotide-adducted magnetic particles. Interaction of amplified products specific to the target organism with the beads in a rotating magnetic field allows for near instantaneous (<30 s) detection based on hybridization-induced aggregation (HIA) of the particles and simple optical analysis. The integration of PCR with this rapid, sequence-specific DNA detection method on a single microdevice presents the possibility of creating POC NAT systems that are low cost, easy-to-use, and involve minimal external hardware.

  1. 3D bioprinting of tissues and organs.

    PubMed

    Murphy, Sean V; Atala, Anthony

    2014-08-01

    Additive manufacturing, otherwise known as three-dimensional (3D) printing, is driving major innovations in many areas, such as engineering, manufacturing, art, education and medicine. Recent advances have enabled 3D printing of biocompatible materials, cells and supporting components into complex 3D functional living tissues. 3D bioprinting is being applied to regenerative medicine to address the need for tissues and organs suitable for transplantation. Compared with non-biological printing, 3D bioprinting involves additional complexities, such as the choice of materials, cell types, growth and differentiation factors, and technical challenges related to the sensitivities of living cells and the construction of tissues. Addressing these complexities requires the integration of technologies from the fields of engineering, biomaterials science, cell biology, physics and medicine. 3D bioprinting has already been used for the generation and transplantation of several tissues, including multilayered skin, bone, vascular grafts, tracheal splints, heart tissue and cartilaginous structures. Other applications include developing high-throughput 3D-bioprinted tissue models for research, drug discovery and toxicology.

  2. An integrated approach based on multiplexed protein array and iTRAQ labeling for in-depth identification of pathways associated to IVF outcome.

    PubMed

    Severino, Valeria; Malorni, Livia; Cicatiello, Anna Emilia; D'Esposito, Vittoria; Longobardi, Salvatore; Colacurci, Nicola; Miraglia, Nadia; Sannolo, Nicola; Farina, Annarita; Chambery, Angela

    2013-01-01

    The emergence of high-throughput protein quantification methodologies has enabled the comprehensive characterization by longitudinal and cross-sectional studies of biological fluids under physiological and pathological conditions. In particular, the simultaneous investigation of cytokines and growth factors signaling pathways and their associated downstream effectors by integrated multiplexed approaches offers a powerful strategy to gain insights into biological networks and processes in living systems. A growing body of research indicates that bioactive molecules of human reproductive fluids, including human follicular fluid (hFF), may affect oocyte quality, fertilization and embryo development, thus potentially influencing the physiopathology of pregnancy-related conditions. In this work, an iTRAQ labeling strategy has been complemented with a multiplexed protein array approach to analyze hFFs with the aim to investigate biological processes and pathways related to in vitro fertilization (IVF) outcome. The iTRAQ labeling strategy lead to the quantification of 89 proteins, 30 of which were differentially expressed in hFFs with successful compared to unsuccessful IVF outcome. The targeted study, based on multiplexed antibody protein arrays, allowed the simultaneous quantification of 27 low abundance proteins, including growth factors, chemokines and cytokines endowed with pro- and anti-inflammatory activity. A significant number of differentially regulated proteins were involved in biological functions related to blood coagulation, acute phase response signaling and complement system. Overall, the present results provide an integrated overview of protein changes in hFFs associated to IVF outcome, thus improving current knowledge in reproductive medicine and fertility research.

  3. Charging YOYO-1 on capillary wall for online DNA intercalation and integrating this approach with multiplex PCR and bare narrow capillary-hydrodynamic chromatography for online DNA analysis.

    PubMed

    Chen, Huang; Zhu, Zaifang; Lu, Joann Juan; Liu, Shaorong

    2015-02-03

    Multiplex polymerase chain reaction (PCR) has been widely utilized for high-throughput pathogen identification. Often, a dye is used to intercalate the amplified DNA fragments, and identifications of the pathogens are carried out by DNA melting curve analysis or gel electrophoresis. Integrating DNA amplification and identification is a logic path toward maximizing the benefit of multiplex PCR. Although PCR and gel electrophoresis have been integrated, replenishing the gels after each run is tedious and time-consuming. In this technical note, we develop an approach to address this issue. We perform multiplex PCR inside a capillary, transfer the amplified fragments to a bare narrow capillary, and measure their lengths online using bare narrow capillary-hydrodynamic chromatography (BaNC-HDC), a new technique recently developed in our laboratory for free-solution DNA separation. To intercalate the DNA with YOYO-1 (a fluorescent dye) for BaNC-HDC, we flush the capillary column with a YOYO-1 solution; positively charged YOYO-1 is adsorbed (or charged) onto the negatively charged capillary wall. As DNA molecules are driven down the column for separation, they react with the YOYO-1 stored on the capillary wall and are online-intercalated with the dye. With a single YOYO-1 charging, the column can be used for more than 40 runs, although the fluorescence signal intensities of the DNA peaks decrease gradually. Although the dye-DNA intercalation occurs during the separation, it does not affect the retention times, separation efficiencies, or resolutions.