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Sample records for 3d optical surface

  1. Optical 3D surface digitizing in forensic medicine: 3D documentation of skin and bone injuries.

    PubMed

    Thali, Michael J; Braun, Marcel; Dirnhofer, Richard

    2003-11-26

    Photography process reduces a three-dimensional (3D) wound to a two-dimensional level. If there is a need for a high-resolution 3D dataset of an object, it needs to be three-dimensionally scanned. No-contact optical 3D digitizing surface scanners can be used as a powerful tool for wound and injury-causing instrument analysis in trauma cases. The 3D skin wound and a bone injury documentation using the optical scanner Advanced TOpometric Sensor (ATOS II, GOM International, Switzerland) will be demonstrated using two illustrative cases. Using this 3D optical digitizing method the wounds (the virtual 3D computer model of the skin and the bone injuries) and the virtual 3D model of the injury-causing tool are graphically documented in 3D in real-life size and shape and can be rotated in the CAD program on the computer screen. In addition, the virtual 3D models of the bone injuries and tool can now be compared in a 3D CAD program against one another in virtual space, to see if there are matching areas. Further steps in forensic medicine will be a full 3D surface documentation of the human body and all the forensic relevant injuries using optical 3D scanners.

  2. 3D Human cartilage surface characterization by optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Brill, Nicolai; Riedel, Jörn; Schmitt, Robert; Tingart, Markus; Truhn, Daniel; Pufe, Thomas; Jahr, Holger; Nebelung, Sven

    2015-10-01

    Early diagnosis and treatment of cartilage degeneration is of high clinical interest. Loss of surface integrity is considered one of the earliest and most reliable signs of degeneration, but cannot currently be evaluated objectively. Optical Coherence Tomography (OCT) is an arthroscopically available light-based non-destructive real-time imaging technology that allows imaging at micrometre resolutions to millimetre depths. As OCT-based surface evaluation standards remain to be defined, the present study investigated the diagnostic potential of 3D surface profile parameters in the comprehensive evaluation of cartilage degeneration. To this end, 45 cartilage samples of different degenerative grades were obtained from total knee replacements (2 males, 10 females; mean age 63.8 years), cut to standard size and imaged using a spectral-domain OCT device (Thorlabs, Germany). 3D OCT datasets of 8  ×  8, 4  ×  4 and 1  ×  1 mm (width  ×  length) were obtained and pre-processed (image adjustments, morphological filtering). Subsequent automated surface identification algorithms were used to obtain the 3D primary profiles, which were then filtered and processed using established algorithms employing ISO standards. The 3D surface profile thus obtained was used to calculate a set of 21 3D surface profile parameters, i.e. height (e.g. Sa), functional (e.g. Sk), hybrid (e.g. Sdq) and segmentation-related parameters (e.g. Spd). Samples underwent reference histological assessment according to the Degenerative Joint Disease classification. Statistical analyses included calculation of Spearman’s rho and assessment of inter-group differences using the Kruskal Wallis test. Overall, the majority of 3D surface profile parameters revealed significant degeneration-dependent differences and correlations with the exception of severe end-stage degeneration and were of distinct diagnostic value in the assessment of surface integrity. None of the 3D

  3. Optical 3D shape, surface, and material analysis

    NASA Astrophysics Data System (ADS)

    Tiziani, Hans J.

    2001-06-01

    Different techniques are available for macro- and micro- topometry. The methods are basically known but their industrial implementation requires robust measuring systems, where calibration is an important necessity. Different techniques will be presented. New elements such as liquid crystal displays and micromirror devices are available leading to new applications to be discussed. Combinative methods and integration in measuring systems becomes interesting. The state of the art and new developments will be presented. Together with calibration for 3D-shock or vibration analysis an object shape measuring systems will be directly combined with a vibration measuring system.

  4. A 3D optical study of Low Surface Brightness galaxies

    NASA Astrophysics Data System (ADS)

    Chemin, L.; Amram, P.; Carignan, C.; Balkowski, C.; van Driel, W.; Cayatte, V.; Hernandez, O.

    2004-12-01

    Integral field spectroscopy observations of the ionized gas in Low Surface Brightness Galaxies (LSBs) are presented. The goal of this study is to map their kinematics at high angular resolution and to study their dark matter (DM) distribution. For that purpose, we have used Fabry-Perot observations obtained at the CFH and ESO 3.6m telescopes. The new contribution of highly resolved velocity fields is crucial to study the role of non-circular motions on the dynamics of LSBs, and particularly on the shape of their DM halo profile (cusp- or core- dominated halo). Here are shown some examples of galaxies in which such motions exist in their central parts and prevent from determining the accurate shape of their DM halo.

  5. Simultaneous calculation of three optical surfaces in the 3D SMS freeform RXI optic

    NASA Astrophysics Data System (ADS)

    Sorgato, Simone; Chaves, Julio; Mohedano, Rubén.; Hernández, Maikel; Blen, José; Benitez, Pablo; Miñano, Juan C.; Grabovickic, Dejan; Thienpont, Hugo; Duerr, Fabian

    2016-09-01

    The Freeform RXI collimator is a remarkable example of advanced nonimaging device designed with the 3D Simultaneous Multiple Surface (SMS) Method. In the original design, two (the front refracting surface and the back mirror) of the three optical surfaces of the RXI are calculated simultaneously and one (the cavity surrounding the source) is fixed by the designer. As a result, the RXI perfectly couples two input wavefronts (coming from the edges of the extended LED source) with two output wavefronts (defining the output beam). This allows for LED lamps able to produce controlled intensity distributions, which can and have been successfully applied to demanding applications like high- and low-beams for Automotive Lighting. Nevertheless, current trends in this field are moving towards smaller headlamps with more shape constraints driven by car design. We present an improved version of the 3D RXI in which also the cavity surface is computed during the design, so that there are three freeform surfaces calculated simultaneously and an additional degree of freedom for controlling the light emission: now the RXI can perfectly couple three input wavefronts with three output wavefronts. The enhanced control over ray beams allows for improved light homogeneity and better pattern definition.

  6. 3D shape measurement of optical free-form surface based on fringe projection

    NASA Astrophysics Data System (ADS)

    Li, Shaohui; Liu, Shugui; Zhang, Hongwei

    2011-05-01

    Present a novel method of 3D shape measurement of optical free-from surface based on fringe projection. A virtual reference surface is proposed which can be used to improve the detection efficiency and realize the automation of measuring process. Sinusoidal fringe patterns are projected to the high reflected surface of the measured object. The deflection fringe patterns that modulated by the object surface are captured by the CCD camera. The slope information can be obtained by analyzing the relationship between the phase deflectometry and the slope of the object surface. The wave-front reconstruction method is used to reconstruct the surface. With the application of fringe projection technology the accuracy of optical free-form surfaces measurement could reach the level of tens of micrometer or even micrometer.

  7. Extended volume and surface scatterometer for optical characterization of 3D-printed elements

    NASA Astrophysics Data System (ADS)

    Dannenberg, Florian; Uebeler, Denise; Weiß, Jürgen; Pescoller, Lukas; Weyer, Cornelia; Hahlweg, Cornelius

    2015-09-01

    The use of 3d printing technology seems to be a promising way for low cost prototyping, not only of mechanical, but also of optical components or systems. It is especially useful in applications where customized equipment repeatedly is subject to immediate destruction, as in experimental detonics and the like. Due to the nature of the 3D-printing process, there is a certain inner texture and therefore inhomogeneous optical behaviour to be taken into account, which also indicates mechanical anisotropy. Recent investigations are dedicated to quantification of optical properties of such printed bodies and derivation of corresponding optimization strategies for the printing process. Beside mounting, alignment and illumination means, also refractive and reflective elements are subject to investigation. The proposed measurement methods are based on an imaging nearfield scatterometer for combined volume and surface scatter measurements as proposed in previous papers. In continuation of last year's paper on the use of near field imaging, which basically is a reflective shadowgraph method, for characterization of glossy surfaces like printed matter or laminated material, further developments are discussed. The device has been extended for observation of photoelasticity effects and therefore homogeneity of polarization behaviour. A refined experimental set-up is introduced. Variation of plane of focus and incident angle are used for separation of various the images of the layers of the surface under test, cross and parallel polarization techniques are applied. Practical examples from current research studies are included.

  8. Comparative validation of single-shot optical techniques for laparoscopic 3-D surface reconstruction.

    PubMed

    Maier-Hein, L; Groch, A; Bartoli, A; Bodenstedt, S; Boissonnat, G; Chang, P-L; Clancy, N T; Elson, D S; Haase, S; Heim, E; Hornegger, J; Jannin, P; Kenngott, H; Kilgus, T; Müller-Stich, B; Oladokun, D; Röhl, S; Dos Santos, T R; Schlemmer, H-P; Seitel, A; Speidel, S; Wagner, M; Stoyanov, D

    2014-10-01

    Intra-operative imaging techniques for obtaining the shape and morphology of soft-tissue surfaces in vivo are a key enabling technology for advanced surgical systems. Different optical techniques for 3-D surface reconstruction in laparoscopy have been proposed, however, so far no quantitative and comparative validation has been performed. Furthermore, robustness of the methods to clinically important factors like smoke or bleeding has not yet been assessed. To address these issues, we have formed a joint international initiative with the aim of validating different state-of-the-art passive and active reconstruction methods in a comparative manner. In this comprehensive in vitro study, we investigated reconstruction accuracy using different organs with various shape and texture and also tested reconstruction robustness with respect to a number of factors like the pose of the endoscope as well as the amount of blood or smoke present in the scene. The study suggests complementary advantages of the different techniques with respect to accuracy, robustness, point density, hardware complexity and computation time. While reconstruction accuracy under ideal conditions was generally high, robustness is a remaining issue to be addressed. Future work should include sensor fusion and in vivo validation studies in a specific clinical context. To trigger further research in surface reconstruction, stereoscopic data of the study will be made publically available at www.open-CAS.com upon publication of the paper.

  9. Assessment of Iterative Closest Point Registration Accuracy for Different Phantom Surfaces Captured by an Optical 3D Sensor in Radiotherapy

    PubMed Central

    Walke, Mathias; Gademann, Günther

    2017-01-01

    An optical 3D sensor provides an additional tool for verification of correct patient settlement on a Tomotherapy treatment machine. The patient's position in the actual treatment is compared with the intended position defined in treatment planning. A commercially available optical 3D sensor measures parts of the body surface and estimates the deviation from the desired position without markers. The registration precision of the in-built algorithm and of selected ICP (iterative closest point) algorithms is investigated on surface data of specially designed phantoms captured by the optical 3D sensor for predefined shifts of the treatment table. A rigid body transform is compared with the actual displacement to check registration reliability for predefined limits. The curvature type of investigated phantom bodies has a strong influence on registration result which is more critical for surfaces of low curvature. We investigated the registration accuracy of the optical 3D sensor for the chosen phantoms and compared the results with selected unconstrained ICP algorithms. Safe registration within the clinical limits is only possible for uniquely shaped surface regions, but error metrics based on surface normals improve translational registration. Large registration errors clearly hint at setup deviations, whereas small values do not guarantee correct positioning. PMID:28163773

  10. State of the art of compact optical 3D profile measurement apparatuses: from outer surface to inner surface measurement

    NASA Astrophysics Data System (ADS)

    Yoshizawa, Toru; Wakayama, Toshitaka

    2013-06-01

    This paper is not an original paper, but a review paper passed on our previous papers. We have been developing a few apparatuses for 2D and/or 3D profile measurement because these systems, especially 3D profiling systems, have become indispensable tools in manufacturing industry. However, in surface profile measurement, conventional systems have several short comings including being very large in size and heavy in weight. Therefore we propose to realize a compact portable apparatus on the basis of pattern projection method using a single MEMS mirror scanning. On the other hand, in the case of inner profile measurement for pipes or tubes, we propose to use optical section method by means of disk beam produced by a conical mirror. In these systems development of elements and devices such as a MEMS mirror and/or cone mirror play important role to apply our fundamental principles to practical apparatuses. We introduce the state of the art of these systems including commercialized products for practical purpose.

  11. A 3D Optical Surface Profilometer Using a Dual-Frequency Liquid Crystal-Based Dynamic Fringe Pattern Generator

    PubMed Central

    Joo, Kyung-Il; Kim, Mugeon; Park, Min-Kyu; Park, Heewon; Kim, Byeonggon; Hahn, JoonKu; Kim, Hak-Rin

    2016-01-01

    We propose a liquid crystal (LC)-based 3D optical surface profilometer that can utilize multiple fringe patterns to extract an enhanced 3D surface depth profile. To avoid the optical phase ambiguity and enhance the 3D depth extraction, 16 interference patterns were generated by the LC-based dynamic fringe pattern generator (DFPG) using four-step phase shifting and four-step spatial frequency varying schemes. The DFPG had one common slit with an electrically controllable birefringence (ECB) LC mode and four switching slits with a twisted nematic LC mode. The spatial frequency of the projected fringe pattern could be controlled by selecting one of the switching slits. In addition, moving fringe patterns were obtainable by applying voltages to the ECB LC layer, which varied the phase difference between the common and the selected switching slits. Notably, the DFPG switching time required to project 16 fringe patterns was minimized by utilizing the dual-frequency modulation of the driving waveform to switch the LC layers. We calculated the phase modulation of the DFPG and reconstructed the depth profile of 3D objects using a discrete Fourier transform method and geometric optical parameters. PMID:27801812

  12. Innovative simultaneous confocal full-field 3D surface profilometry for in situ automatic optical inspection (AOI)

    NASA Astrophysics Data System (ADS)

    Chen, Liang-Chia; Chang, Yi-Wei

    2010-06-01

    Rapid acquisition of surface 3D contour information using optical detection has attracted tremendous interest in the field of automatic optical inspection (AOI) and how to avoid or minimize environmental vibration or disturbance has become a critical issue in in situ inspection. Owing to its high longitudinal measurability and excellent vertical resolution, optical confocal microscopy has become extremely important for surface profilometry. This study presents a novel simultaneous confocal full-field 3D surface profilometer using structured fringe projection. The developed confocal optical system is capable of acquiring multiple images at various object depths to perform surface 3D reconstruction by a single image shot without the need for time-consuming vertical scanning. In this method, four conjugate image-sensing modules are configured at four different designated focusing positions, which are controlled by a specially designed beam-splitting optical module. A focal-depth response (FDR) curve can be established by fitting the four focus measurements obtained from these designated positions to achieve simultaneous confocal vertical scanning. In addition, using the principle of optical grating projection, a structured fringe pattern is generated for lateral scanning to enhance the spatial measurement resolution. To examine the performance of the developed system, an accurate step-height target and some industrial micro semiconductor components were measured. The results show that the depth measurement resolution can reach up to 0.1 µm and the maximum measurement error is within 1.5% of the overall range, indicating both accuracy and repeatability of the proposed confocal measurement approach.

  13. 3D nanopillar optical antenna photodetectors.

    PubMed

    Senanayake, Pradeep; Hung, Chung-Hong; Shapiro, Joshua; Scofield, Adam; Lin, Andrew; Williams, Benjamin S; Huffaker, Diana L

    2012-11-05

    We demonstrate 3D surface plasmon photoresponse in nanopillar arrays resulting in enhanced responsivity due to both Localized Surface Plasmon Resonances (LSPRs) and Surface Plasmon Polariton Bloch Waves (SPP-BWs). The LSPRs are excited due to a partial gold shell coating the nanopillar which acts as a 3D Nanopillar Optical Antenna (NOA) in focusing light into the nanopillar. Angular photoresponse measurements show that SPP-BWs can be spectrally coincident with LSPRs to result in a x2 enhancement in responsivity at 1180 nm. Full-wave Finite Difference Time Domain (FDTD) simulations substantiate both the spatial and spectral coupling of the SPP-BW / LSPR for enhanced absorption and the nature of the LSPR. Geometrical control of the 3D NOA and the self-aligned metal hole lattice allows the hybridization of both localized and propagating surface plasmon modes for enhanced absorption. Hybridized plasmonic modes opens up new avenues in optical antenna design in nanoscale photodetectors.

  14. High-resolution 3-D imaging of surface damage sites in fused silica with Optical Coherence Tomography

    SciTech Connect

    Guss, G; Bass, I; Hackel, R; Mailhiot, C; Demos, S G

    2007-10-30

    In this work, we present the first successful demonstration of a non-contact technique to precisely measure the 3D spatial characteristics of laser induced surface damage sites in fused silica for large aperture laser systems by employing Optical Coherence Tomography (OCT). What makes OCT particularly interesting in the characterization of optical materials for large aperture laser systems is that its axial resolution can be maintained with working distances greater than 5 cm, whether viewing through air or through the bulk of thick optics. Specifically, when mitigating surface damage sites against further growth by CO{sub 2} laser evaporation of the damage, it is important to know the depth of subsurface cracks below the damage site. These cracks are typically obscured by the damage rubble when imaged from above the surface. The results to date clearly demonstrate that OCT is a unique and valuable tool for characterizing damage sites before and after the mitigation process. We also demonstrated its utility as an in-situ diagnostic to guide and optimize our process when mitigating surface damage sites on large, high-value optics.

  15. Modular optical topometric sensor for 3D acquisition of human body surfaces and long-term monitoring of variations.

    PubMed

    Bischoff, Guido; Böröcz, Zoltan; Proll, Christian; Kleinheinz, Johannes; von Bally, Gert; Dirksen, Dieter

    2007-08-01

    Optical topometric 3D sensors such as laser scanners and fringe projection systems allow detailed digital acquisition of human body surfaces. For many medical applications, however, not only the current shape is important, but also its changes, e.g., in the course of surgical treatment. In such cases, time delays of several months between subsequent measurements frequently occur. A modular 3D coordinate measuring system based on the fringe projection technique is presented that allows 3D coordinate acquisition including calibrated color information, as well as the detection and visualization of deviations between subsequent measurements. In addition, parameters describing the symmetry of body structures are determined. The quantitative results of the analysis may be used as a basis for objective documentation of surgical therapy. The system is designed in a modular way, and thus, depending on the object of investigation, two or three cameras with different capabilities in terms of resolution and color reproduction can be utilized to optimize the set-up.

  16. Initial Validation and Clinical Experience with 3D Optical-Surface-Guided Whole Breast Irradiation of Breast Cancer

    PubMed Central

    Li, S.; DeWeese, T.; Movsas, B.; Frassica, Deborah; Liu, Dezhi; Kim, Jinkoo; Chen, Qing; Walker, Eleanor

    2015-01-01

    We had introduced 3D optical surface-guided radiotherapy (SGRT) of the breast cancer (BC). We then initiated the feasibility, accuracy, and precision studies of stereovision in detection of any breast displacement through the course of treatment for total thirty breasts undertaken whole breast irradiation (WBI). In the SGRT, CT-based plan data were parsed into an in-house computer program through which the reference surfaces were generated in 3D video format. When patients were positioned on treatment Tables, real-time stereovisions were rapidly acquired while the live surface tracking shown steady thorax motion. The real-time surface images were automatically aligned with the reference surface and detected shape and location changes of the breast were online corrected through the Table and beam adjustments. Accumulated dose to each patient was computed according to the frequency distribution of the measured breast locations during beam on time. Application of SGRT had diminished large skin-marking errors of >5-mm and daily breast-setup errors of >10-mm that occurred on half of cases. Accuracy (mean) and precision (two standard deviations) of the breast displacements across the tangential field edges in the (U, V) directions were improved from (−0.5 ± 8.8, 2.2 ± 10.8) mm in conventional setup to (0.4 ± 4.6, 0.7 ± 4.4) mm in the final position while intra-fractional motion contributed only (0.1 ± 2.8, 0.0 ± 2.2) mm in free breathing. Dose uniformity and coverage to targets had both been increased by up to 10% and the lung or heart intersections have been decreased by half of those volumes if they were irradiated at the initial positions. SGRT of BC appears to be feasible regardless of skin tones, as fast as a snapshot for 3D imaging, and very accurate and precise for daily setup of flexible breast targets. Importantly, the technique allows us to verify the breast shape and position during beam-on time. PMID:22181332

  17. Optically rewritable 3D liquid crystal displays.

    PubMed

    Sun, J; Srivastava, A K; Zhang, W; Wang, L; Chigrinov, V G; Kwok, H S

    2014-11-01

    Optically rewritable liquid crystal display (ORWLCD) is a concept based on the optically addressed bi-stable display that does not need any power to hold the image after being uploaded. Recently, the demand for the 3D image display has increased enormously. Several attempts have been made to achieve 3D image on the ORWLCD, but all of them involve high complexity for image processing on both hardware and software levels. In this Letter, we disclose a concept for the 3D-ORWLCD by dividing the given image in three parts with different optic axis. A quarter-wave plate is placed on the top of the ORWLCD to modify the emerging light from different domains of the image in different manner. Thereafter, Polaroid glasses can be used to visualize the 3D image. The 3D image can be refreshed, on the 3D-ORWLCD, in one-step with proper ORWLCD printer and image processing, and therefore, with easy image refreshing and good image quality, such displays can be applied for many applications viz. 3D bi-stable display, security elements, etc.

  18. Optical microcavity scanning 3D tomography.

    PubMed

    Di Donato, Andrea; Criante, Luigino; LoTurco, Sara; Farina, Marco

    2014-10-01

    A scanning optical microcavity is exploited to achieve lens-free 3D tomography of microfluidic channels. The microcavity, powered by a low-coherence source, is realized by approaching a cleaved fiber to few tens of micrometers over the sample. The interference of scattered waves inside the cavity shapes the transverse field distribution by focusing the beam and overcoming the diffraction limit due to the optical-fiber numerical aperture. The focusing effect is also preserved in the inner layers of the sample, allowing optical 3D tomography. Analysis of microfluidic channels was demonstrated through this noninvasive technique. Although the experimental setup recalls the well-known fiber-optic Fourier-domain common-path optical coherence tomography, the proposed method has intrinsic characteristics that distinguish it from the former one.

  19. 3D integral imaging with optical processing

    NASA Astrophysics Data System (ADS)

    Martínez-Corral, Manuel; Martínez-Cuenca, Raúl; Saavedra, Genaro; Javidi, Bahram

    2008-04-01

    Integral imaging (InI) systems are imaging devices that provide auto-stereoscopic images of 3D intensity objects. Since the birth of this new technology, InI systems have faced satisfactorily many of their initial drawbacks. Basically, two kind of procedures have been used: digital and optical procedures. The "3D Imaging and Display Group" at the University of Valencia, with the essential collaboration of Prof. Javidi, has centered its efforts in the 3D InI with optical processing. Among other achievements, our Group has proposed the annular amplitude modulation for enlargement of the depth of field, dynamic focusing for reduction of the facet-braiding effect, or the TRES and MATRES devices to enlarge the viewing angle.

  20. 3D optical measuring technologies and systems

    NASA Astrophysics Data System (ADS)

    Chugui, Yuri V.

    2005-02-01

    The results of the R & D activity of TDI SIE SB RAS in the field of the 3D optical measuring technologies and systems for noncontact 3D optical dimensional inspection applied to atomic and railway industry safety problems are presented. This activity includes investigations of diffraction phenomena on some 3D objects, using the original constructive calculation method. The efficient algorithms for precise determining the transverse and longitudinal sizes of 3D objects of constant thickness by diffraction method, peculiarities on formation of the shadow and images of the typical elements of the extended objects were suggested. Ensuring the safety of nuclear reactors and running trains as well as their high exploitation reliability requires a 100% noncontact precise inspection of geometrical parameters of their components. To solve this problem we have developed methods and produced the technical vision measuring systems LMM, CONTROL, PROFIL, and technologies for noncontact 3D dimensional inspection of grid spacers and fuel elements for the nuclear reactor VVER-1000 and VVER-440, as well as automatic laser diagnostic COMPLEX for noncontact inspection of geometric parameters of running freight car wheel pairs. The performances of these systems and the results of industrial testing are presented and discussed. The created devices are in pilot operation at Atomic and Railway Companies.

  1. Nonlaser-based 3D surface imaging

    SciTech Connect

    Lu, Shin-yee; Johnson, R.K.; Sherwood, R.J.

    1994-11-15

    3D surface imaging refers to methods that generate a 3D surface representation of objects of a scene under viewing. Laser-based 3D surface imaging systems are commonly used in manufacturing, robotics and biomedical research. Although laser-based systems provide satisfactory solutions for most applications, there are situations where non laser-based approaches are preferred. The issues that make alternative methods sometimes more attractive are: (1) real-time data capturing, (2) eye-safety, (3) portability, and (4) work distance. The focus of this presentation is on generating a 3D surface from multiple 2D projected images using CCD cameras, without a laser light source. Two methods are presented: stereo vision and depth-from-focus. Their applications are described.

  2. Optical 3D Deformation Measurement Utilizing Non-planar Surface for the Development of an “Intelligent Tire”

    NASA Astrophysics Data System (ADS)

    Matsuzaki, Ryosuke; Hiraoka, Naoki; Todoroki, Akira; Mizutani, Yoshihiro

    Intelligent tires, also known as smart tires, are equipped with sensors to monitor the strain of the interior surface and the rolling radius of tire, and are expected to improve the reliability of tires and tire control systems such as anti-lock braking systems (ABS). However, the high stiffness of an attached sensor like a strain gauge causes sensors to debond from the tire rubber. In the present study, a novel optical method is used for the concurrent monitoring of in-plane strain and out-of-plane displacement (rolling radius) utilizing the non-planar surface of the monitoring object. The optical method enables noncontact measurement of strain distribution. The in-plane strain and out-of-plane displacement are calculated by using image processing with an image of the interior surface of a tire that is taken with a single CCD camera fixed on the wheel rim. This new monitoring system is applied to an aluminum beam and a commercially available radial tire. As a result, the monitoring system provides concurrent measurement of in-plane strain, out-of-plane displacement and tire pressure, and is shown to be an effective monitoring system for intelligent tires.

  3. A novel method of creating a surface micromachined 3D optical assembly for MEMS-based miniaturized FTIR spectrometers

    NASA Astrophysics Data System (ADS)

    Reyes, D.; Schildkraut, E. R.; Kim, J.; Connors, R. F.; Kotidis, P.; Cavicchio, D. J.

    2008-02-01

    This paper describes design, fabrication, and characterization of a miniaturized, Fourier transform infrared (FTIR) spectrometer for the detection and identification of toxic or flammable gases. By measuring the absorption by the target material of IR radiation, unambiguous detection and identification can be achieved. The key component of the device is a micromachined Michelson interferometer capable of modulating light in the 2 - 14 μm spectral region. Two major technical achievements associated with developing a MEMS interferometer module are discussed: development of a micromirror assembly having an order of magnitude larger modulation stroke to approach laboratory instrument-grade spectral resolutions; and assembly of monolithic, millimeter-scale optical components using multi-layer surface micromachining techniques to produce an extremely low cost MEMS interferometer, which has an unprecedented optical throughput. We have manufactured and tested the device. Reported optical characterization results include a precisely aligned, static interferogram acquired from an assembled Michelson interferometer using visible light wavelengths, which promises a high sensitivity FTIR spectrometer for its size.

  4. Methods for comparing 3D surface attributes

    NASA Astrophysics Data System (ADS)

    Pang, Alex; Freeman, Adam

    1996-03-01

    A common task in data analysis is to compare two or more sets of data, statistics, presentations, etc. A predominant method in use is side-by-side visual comparison of images. While straightforward, it burdens the user with the task of discerning the differences between the two images. The user if further taxed when the images are of 3D scenes. This paper presents several methods for analyzing the extent, magnitude, and manner in which surfaces in 3D differ in their attributes. The surface geometry are assumed to be identical and only the surface attributes (color, texture, etc.) are variable. As a case in point, we examine the differences obtained when a 3D scene is rendered progressively using radiosity with different form factor calculation methods. The comparison methods include extensions of simple methods such as mapping difference information to color or transparency, and more recent methods including the use of surface texture, perturbation, and adaptive placements of error glyphs.

  5. Fiber optic coherent laser radar 3D vision system

    SciTech Connect

    Clark, R.B.; Gallman, P.G.; Slotwinski, A.R.; Wagner, K.; Weaver, S.; Xu, Jieping

    1996-12-31

    This CLVS will provide a substantial advance in high speed computer vision performance to support robotic Environmental Management (EM) operations. This 3D system employs a compact fiber optic based scanner and operator at a 128 x 128 pixel frame at one frame per second with a range resolution of 1 mm over its 1.5 meter working range. Using acousto-optic deflectors, the scanner is completely randomly addressable. This can provide live 3D monitoring for situations where it is necessary to update once per second. This can be used for decontamination and decommissioning operations in which robotic systems are altering the scene such as in waste removal, surface scarafacing, or equipment disassembly and removal. The fiber- optic coherent laser radar based system is immune to variations in lighting, color, or surface shading, which have plagued the reliability of existing 3D vision systems, while providing substantially superior range resolution.

  6. Multi-surface and multi-field co-segmentation of 3-D retinal optical coherence tomography.

    PubMed

    Bogunovic, Hrvoje; Sonka, Milan; Kwon, Young H; Kemp, Pavlina; Abramoff, Michael D; Wu, Xiaodong

    2014-12-01

    When segmenting intraretinal layers from multiple optical coherence tomography (OCT) images forming a mosaic or a set of repeated scans, it is attractive to exploit the additional information from the overlapping areas rather than discarding it as redundant, especially in low contrast and noisy images. However, it is currently not clear how to effectively combine the multiple information sources available in the areas of overlap. In this paper, we propose a novel graph-theoretic method for multi-surface multi-field co-segmentation of intraretinal layers, assuring consistent segmentation of the fields across the overlapped areas. After 2-D en-face alignment, all the fields are segmented simultaneously, imposing a priori soft interfield-intrasurface constraints for each pair of overlapping fields. The constraints penalize deviations from the expected surface height differences, taken to be the depth-axis shifts that produce the maximum cross-correlation of pairwise-overlapped areas. The method's accuracy and reproducibility are evaluated qualitatively and quantitatively on 212 OCT images (20 nine-field, 32 single-field acquisitions) from 26 patients with glaucoma. Qualitatively, the obtained thickness maps show no stitching artifacts, compared to pronounced stitches when the fields are segmented independently. Quantitatively, two ophthalmologists manually traced four intraretinal layers on 10 patients, and the average error ( 4.58 ±1.46 μm) was comparable to the average difference between the observers ( 5.86±1.72 μm). Furthermore, we show the benefit of the proposed approach in co-segmenting longitudinal scans. As opposed to segmenting layers in each of the fields independently, the proposed co-segmentation method obtains consistent segmentations across the overlapped areas, producing accurate, reproducible, and artifact-free results.

  7. Multi-Surface and Multi-Field Co-Segmentation of 3-D Retinal Optical Coherence Tomography

    PubMed Central

    Sonka, Milan; Kwon, Young H.; Kemp, Pavlina; Abràmoff, Michael D.; Wu, Xiaodong

    2015-01-01

    When segmenting intraretinal layers from multiple optical coherence tomography (OCT) images forming a mosaic or a set of repeated scans, it is attractive to exploit the additional information from the overlapping areas rather than discarding it as redundant, especially in low contrast and noisy images. However, it is currently not clear how to effectively combine the multiple information sources available in the areas of overlap. In this paper, we propose a novel graph-theoretic method for multi-surface multi-field co-segmentation of intraretinal layers, assuring consistent segmentation of the fields across the overlapped areas. After 2-D en-face alignment, all the fields are segmented simultaneously, imposing a priori soft interfield-intrasurface constraints for each pair of overlapping fields. The constraints penalize deviations from the expected surface height differences, taken to be the depth-axis shifts that produce the maximum cross-correlation of pairwise-overlapped areas. The method’s accuracy and reproducibility are evaluated qualitatively and quantitatively on 212 OCT images (20 nine-field, 32 single-field acquisitions) from 26 patients with glaucoma. Qualitatively, the obtained thickness maps show no stitching artifacts, compared to pronounced stitches when the fields are segmented independently. Quantitatively, two ophthalmologists manually traced four intraretinal layers on 10 patients, and the average error (4.58±1.46 μm) was comparable to the average difference between the observers (5.86±1.72 μm). Furthermore, we show the benefit of the proposed approach in co-segmenting longitudinal scans. As opposed to segmenting layers in each of the fields independently, the proposed co-segmentation method obtains consistent segmentations across the overlapped areas, producing accurate, reproducible, and artifact-free results. PMID:25020067

  8. High-speed optical 3D sensing and its applications

    NASA Astrophysics Data System (ADS)

    Watanabe, Yoshihiro

    2016-12-01

    This paper reviews high-speed optical 3D sensing technologies for obtaining the 3D shape of a target using a camera. The focusing speed is from 100 to 1000 fps, exceeding normal camera frame rates, which are typically 30 fps. In particular, contactless, active, and real-time systems are introduced. Also, three example applications of this type of sensing technology are introduced, including surface reconstruction from time-sequential depth images, high-speed 3D user interaction, and high-speed digital archiving.

  9. Polarized 3D Raman and nanoscale near-field optical microscopy of optically inscribed surface relief gratings: chromophore orientation in azo-doped polymer films.

    PubMed

    Di Florio, Giuseppe; Bründermann, Erik; Yadavalli, Nataraja Sekhar; Santer, Svetlana; Havenith, Martina

    2014-03-14

    We have used polarized confocal Raman microspectroscopy and scanning near-field optical microscopy with a resolution of 60 nm to characterize photoinscribed grating structures of azobenzene doped polymer films on a glass support. Polarized Raman microscopy allowed determining the reorientation of the chromophores as a function of the grating phase and penetration depth of the inscribing laser in three dimensions. We found periodic patterns, which are not restricted to the surface alone, but appear also well below the surface in the bulk of the material. Near-field optical microscopy with nanoscale resolution revealed lateral two-dimensional optical contrast, which is not observable by atomic force and Raman microscopy.

  10. Optical fabrication of lightweighted 3D printed mirrors

    NASA Astrophysics Data System (ADS)

    Herzog, Harrison; Segal, Jacob; Smith, Jeremy; Bates, Richard; Calis, Jacob; De La Torre, Alyssa; Kim, Dae Wook; Mici, Joni; Mireles, Jorge; Stubbs, David M.; Wicker, Ryan

    2015-09-01

    Direct Metal Laser Sintering (DMLS) and Electron Beam Melting (EBM) 3D printing technologies were utilized to create lightweight, optical grade mirrors out of AlSi10Mg aluminum and Ti6Al4V titanium alloys at the University of Arizona in Tucson. The mirror prototypes were polished to meet the λ/20 RMS and λ/4 P-V surface figure requirements. The intent of this project was to design topologically optimized mirrors that had a high specific stiffness and low surface displacement. Two models were designed using Altair Inspire software, and the mirrors had to endure the polishing process with the necessary stiffness to eliminate print-through. Mitigating porosity of the 3D printed mirror blanks was a challenge in the face of reconciling new printing technologies with traditional optical polishing methods. The prototypes underwent Hot Isostatic Press (HIP) and heat treatment to improve density, eliminate porosity, and relieve internal stresses. Metal 3D printing allows for nearly unlimited topological constraints on design and virtually eliminates the need for a machine shop when creating an optical quality mirror. This research can lead to an increase in mirror mounting support complexity in the manufacturing of lightweight mirrors and improve overall process efficiency. The project aspired to have many future applications of light weighted 3D printed mirrors, such as spaceflight. This paper covers the design/fab/polish/test of 3D printed mirrors, thermal/structural finite element analysis, and results.

  11. Multi-resolution optical 3D sensor

    NASA Astrophysics Data System (ADS)

    Kühmstedt, Peter; Heinze, Matthias; Schmidt, Ingo; Breitbarth, Martin; Notni, Gunther

    2007-06-01

    A new multi resolution self calibrating optical 3D measurement system using fringe projection technique named "kolibri FLEX multi" will be presented. It can be utilised to acquire the all around shape of small to medium objects, simultaneously. The basic measurement principle is the phasogrammetric approach /1,2,3/ in combination with the method of virtual landmarks for the merging of the 3D single views. The system consists in minimum of two fringe projection sensors. The sensors are mounted on a rotation stage illuminating the object from different directions. The measurement fields of the sensors can be chosen different, here as an example 40mm and 180mm in diameter. In the measurement the object can be scanned at the same time with these two resolutions. Using the method of virtual landmarks both point clouds are calculated within the same world coordinate system resulting in a common 3D-point cloud. The final point cloud includes the overview of the object with low point density (wide field) and a region with high point density (focussed view) at the same time. The advantage of the new method is the possibility to measure with different resolutions at the same object region without any mechanical changes in the system or data post processing. Typical parameters of the system are: the measurement time is 2min for 12 images and the measurement accuracy is below 3μm up to 10 μm. The flexibility makes the measurement system useful for a wide range of applications such as quality control, rapid prototyping, design and CAD/CAM which will be shown in the paper.

  12. Total body irradiation with a compensator fabricated using a 3D optical scanner and a 3D printer.

    PubMed

    Park, So-Yeon; Kim, Jung-In; Joo, Yoon Ha; Lee, Jung Chan; Park, Jong Min

    2017-05-07

    We propose bilateral total body irradiation (TBI) utilizing a 3D printer and a 3D optical scanner. We acquired surface information of an anthropomorphic phantom with the 3D scanner and fabricated the 3D compensator with the 3D printer, which could continuously compensate for the lateral missing tissue of an entire body from the beam's eye view. To test the system's performance, we measured doses with optically stimulated luminescent dosimeters (OSLDs) as well as EBT3 films with the anthropomorphic phantom during TBI without a compensator, conventional bilateral TBI, and TBI with the 3D compensator (3D TBI). The 3D TBI showed the most uniform dose delivery to the phantom. From the OSLD measurements of the 3D TBI, the deviations between the measured doses and the prescription dose ranged from  -6.7% to 2.4% inside the phantom and from  -2.3% to 0.6% on the phantom's surface. From the EBT3 film measurements, the prescription dose could be delivered to the entire body of the phantom within  ±10% accuracy, except for the chest region, where tissue heterogeneity is extreme. The 3D TBI doses were much more uniform than those of the other irradiation techniques, especially in the anterior-to-posterior direction. The 3D TBI was advantageous, owing to its uniform dose delivery as well as its efficient treatment procedure.

  13. Vision models for 3D surfaces

    NASA Astrophysics Data System (ADS)

    Mitra, Sunanda

    1992-11-01

    Different approaches to computational stereo to represent human stereo vision have been developed over the past two decades. The Marr-Poggio theory of human stereo vision is probably the most widely accepted model of the human stereo vision. However, recently developed motion stereo models which use a sequence of images taken by either a moving camera or a moving object provide an alternative method of achieving multi-resolution matching without the use of Laplacian of Gaussian operators. While using image sequences, the baseline between two camera positions for a image pair is changed for the subsequent image pair so as to achieve different resolution for each image pair. Having different baselines also avoids the inherent occlusion problem in stereo vision models. The advantage of using multi-resolution images acquired by camera positioned at different baselines over those acquired by LOG operators is that one does not have to encounter spurious edges often created by zero-crossings in the LOG operated images. Therefore in designing a computer vision system, a motion stereo model is more appropriate than a stereo vision model. However, in some applications where only a stereo pair of images are available, recovery of 3D surfaces of natural scenes are possible in a computationally efficient manner by using cepstrum matching and regularization techniques. Section 2 of this paper describes a motion stereo model using multi-scale cepstrum matching for the detection of disparity between image pairs in a sequence of images and subsequent recovery of 3D surfaces from depth-map obtained by a non convergent triangulation technique. Section 3 presents a 3D surface recovery technique from a stereo pair using cepstrum matching for disparity detection and cubic B-splines for surface smoothing. Section 4 contains the results of 3D surface recovery using both of the techniques mentioned above. Section 5 discusses the merit of 2D cepstrum matching and cubic B

  14. Automatic respiration tracking for radiotherapy using optical 3D camera

    NASA Astrophysics Data System (ADS)

    Li, Tuotuo; Geng, Jason; Li, Shidong

    2013-03-01

    Rapid optical three-dimensional (O3D) imaging systems provide accurate digitized 3D surface data in real-time, with no patient contact nor radiation. The accurate 3D surface images offer crucial information in image-guided radiation therapy (IGRT) treatments for accurate patient repositioning and respiration management. However, applications of O3D imaging techniques to image-guided radiotherapy have been clinically challenged by body deformation, pathological and anatomical variations among individual patients, extremely high dimensionality of the 3D surface data, and irregular respiration motion. In existing clinical radiation therapy (RT) procedures target displacements are caused by (1) inter-fractional anatomy changes due to weight, swell, food/water intake; (2) intra-fractional variations from anatomy changes within any treatment session due to voluntary/involuntary physiologic processes (e.g. respiration, muscle relaxation); (3) patient setup misalignment in daily reposition due to user errors; and (4) changes of marker or positioning device, etc. Presently, viable solution is lacking for in-vivo tracking of target motion and anatomy changes during the beam-on time without exposing patient with additional ionized radiation or high magnet field. Current O3D-guided radiotherapy systems relay on selected points or areas in the 3D surface to track surface motion. The configuration of the marks or areas may change with time that makes it inconsistent in quantifying and interpreting the respiration patterns. To meet the challenge of performing real-time respiration tracking using O3D imaging technology in IGRT, we propose a new approach to automatic respiration motion analysis based on linear dimensionality reduction technique based on PCA (principle component analysis). Optical 3D image sequence is decomposed with principle component analysis into a limited number of independent (orthogonal) motion patterns (a low dimension eigen-space span by eigen-vectors). New

  15. PLOT3D- DRAWING THREE DIMENSIONAL SURFACES

    NASA Technical Reports Server (NTRS)

    Canright, R. B.

    1994-01-01

    PLOT3D is a package of programs to draw three-dimensional surfaces of the form z = f(x,y). The function f and the boundary values for x and y are the input to PLOT3D. The surface thus defined may be drawn after arbitrary rotations. However, it is designed to draw only functions in rectangular coordinates expressed explicitly in the above form. It cannot, for example, draw a sphere. Output is by off-line incremental plotter or online microfilm recorder. This package, unlike other packages, will plot any function of the form z = f(x,y) and portrays continuous and bounded functions of two independent variables. With curve fitting; however, it can draw experimental data and pictures which cannot be expressed in the above form. The method used is division into a uniform rectangular grid of the given x and y ranges. The values of the supplied function at the grid points (x, y) are calculated and stored; this defines the surface. The surface is portrayed by connecting successive (y,z) points with straight-line segments for each x value on the grid and, in turn, connecting successive (x,z) points for each fixed y value on the grid. These lines are then projected by parallel projection onto the fixed yz-plane for plotting. This program has been implemented on the IBM 360/67 with on-line CDC microfilm recorder.

  16. Surface Plasmons in 3D Topological Insulators

    NASA Astrophysics Data System (ADS)

    Kogar, Anshul; Vig, Sean; Cho, Gil; Thaler, Alexander; Xiao, Yiran; Hughes, Taylor; Wong, Man-Hong; Chiang, Tai-Chang; MacDougall, Greg; Abbamonte, Peter

    2015-03-01

    Most studies of three-dimensional (3D) topological insulators have concentrated on their one-electron properties as exhibited by angle-resolved photoemission spectroscopy (ARPES) or by scanning tunneling microscopy (STM). Many-body interactions are often neglected in the treatment of models of topological insulators, such as in the Kane-Mele and Bernevig-Hughes-Zhang models. Using angle-resolved inelastic electron scattering from the surface, I will present data on the collective mode that owes its existence to the presence of many-body interactions, the surface plasmon (SP), in two known 3D topological insulators, Bi2Se3 and Bi0.5Sb1.5Se1 . 5 + xTe1 . 5 - x. Surprisingly, the SP was prominent even after depressing the Fermi energy into the bulk band gap. Having studied the SP as a function of doping, momentum transfer and its aging properties, I will present evidence to suggest that bulk-surface coupling is crucial in explaining many of its properties. A simple model with dynamic bulk screening will be presented showing qualitative agreement with the observations. Lastly, the relation of the observed surface plasmon to the predicted spin-plasmon mode and to the kinks seen in the electronic dispersion as measured by ARPES will be discussed. The work was supported as part of the Center for Emergent Superconductivity, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science.

  17. Fiber optic coherent laser radar 3d vision system

    SciTech Connect

    Sebastian, R.L.; Clark, R.B.; Simonson, D.L.

    1994-12-31

    Recent advances in fiber optic component technology and digital processing components have enabled the development of a new 3D vision system based upon a fiber optic FMCW coherent laser radar. The approach includes a compact scanner with no moving parts capable of randomly addressing all pixels. The system maintains the immunity to lighting and surface shading conditions which is characteristic of coherent laser radar. The random pixel addressability allows concentration of scanning and processing on the active areas of a scene, as is done by the human eye-brain system.

  18. Continuous Optical 3D Printing of Green Aliphatic Polyurethanes.

    PubMed

    Pyo, Sang-Hyun; Wang, Pengrui; Hwang, Henry H; Zhu, Wei; Warner, John; Chen, Shaochen

    2017-01-11

    Photosensitive diurethanes were prepared from a green chemistry synthesis pathway based on methacrylate-functionalized six-membered cyclic carbonate and biogenic amines. A continuous optical 3D printing method for the diurethanes was developed to create user-defined gradient stiffness and smooth complex surface microstructures in seconds. The green chemistry-derived polyurethane (gPU) showed high optical transparency, and we demonstrate the ability to tune the material stiffness of the printed structure along a gradient by controlling the exposure time and selecting various amine compounds. High-resolution 3D biomimetic structures with smooth curves and complex contours were printed using our gPU. High cell viability (over 95%) was demonstrated during cytocompatibility testing using C3H 10T1/2 cells seeded directly on the printed structures.

  19. Manufacturing: 3D printed micro-optics

    NASA Astrophysics Data System (ADS)

    Juodkazis, Saulius

    2016-08-01

    Uncompromised performance of micro-optical compound lenses has been achieved by high-fidelity shape definition during two-photon absorption microfabrication. The lenses have been made directly onto image sensors and even onto the tip of an optic fibre.

  20. Precision 3-D microscopy with intensity modulated fibre optic scanners

    NASA Astrophysics Data System (ADS)

    Olmos, P.

    2016-01-01

    Optical 3-D imagers constitute a family of precision and useful instruments, easily available on the market in a wide variety of configurations and performances. However, besides their cost they usually provide an image of the object (i.e. a more or less faithful representation of the reality) instead of a truly object's reconstruction. Depending on the detailed working principles of the equipment, this reconstruction may become a challenging task. Here a very simple yet reliable device is described; it is able to form images of opaque objects by illuminating them with an optical fibre and collecting the reflected light with another fibre. Its 3-D capability comes from the spatial filtering imposed by the fibres together with their movement (scanning) along the three directions: transversal (surface) and vertical. This unsophisticated approach allows one to model accurately the entire optical process and to perform the desired reconstruction, finding that information about the surface which is of interest: its profile and its reflectance, ultimately related to the type of material.

  1. 3D-additive manufactured optical mount

    NASA Astrophysics Data System (ADS)

    Mammini, Paul V.; Ciscel, David; Wooten, John

    2015-09-01

    The Area Defense Anti-Munitions (ADAM) is a low cost and effective high power laser weapon system. It's designed to address and negate important threats such as short-range rockets, UAVs, and small boats. Many critical optical components operate in the system. The optics and mounts must accommodate thermal and mechanical stresses, plus maintain an exceptional wave front during operation. Lockheed Martin Space Systems Company (LMSSC) developed, designed, and currently operates ADAM. This paper covers the design and development of a key monolithic, flexured, titanium mirror mount that was manufactured by CalRAM using additive processes.

  2. 3D reconstruction of SEM images by use of optical photogrammetry software.

    PubMed

    Eulitz, Mona; Reiss, Gebhard

    2015-08-01

    Reconstruction of the three-dimensional (3D) surface of an object to be examined is widely used for structure analysis in science and many biological questions require information about their true 3D structure. For Scanning Electron Microscopy (SEM) there has been no efficient non-destructive solution for reconstruction of the surface morphology to date. The well-known method of recording stereo pair images generates a 3D stereoscope reconstruction of a section, but not of the complete sample surface. We present a simple and non-destructive method of 3D surface reconstruction from SEM samples based on the principles of optical close range photogrammetry. In optical close range photogrammetry a series of overlapping photos is used to generate a 3D model of the surface of an object. We adapted this method to the special SEM requirements. Instead of moving a detector around the object, the object itself was rotated. A series of overlapping photos was stitched and converted into a 3D model using the software commonly used for optical photogrammetry. A rabbit kidney glomerulus was used to demonstrate the workflow of this adaption. The reconstruction produced a realistic and high-resolution 3D mesh model of the glomerular surface. The study showed that SEM micrographs are suitable for 3D reconstruction by optical photogrammetry. This new approach is a simple and useful method of 3D surface reconstruction and suitable for various applications in research and teaching.

  3. 3D printed long period gratings for optical fibers.

    PubMed

    Iezzi, Victor Lambin; Boisvert, Jean-Sébastien; Loranger, Sébastien; Kashyap, Raman

    2016-04-15

    We demonstrate a simple technique for implementing long period grating (LPG) structures by the use of a 3D printer. This Letter shows a way of manipulating the mode coupling within an optical fiber by applying stress through an external 3D printed periodic structure. Different LPG lengths and periods have been studied, as well as the effect of the applied stress on the coupling efficiency from the fundamental mode to cladding modes. The technique is very simple, highly flexible, affordable, and easy to implement without the need of altering the optical fiber. This Letter is part of a growing line of interest in the use of 3D printers for optical applications.

  4. OCT 3-D surface topography of isolated human crystalline lenses

    PubMed Central

    Sun, Mengchan; Birkenfeld, Judith; de Castro, Alberto; Ortiz, Sergio; Marcos, Susana

    2014-01-01

    Quantitative 3-D Optical Coherence Tomography was used to measure surface topography of 36 isolated human lenses, and to evaluate the relationship between anterior and posterior lens surface shape and their changes with age. All lens surfaces were fitted to 6th order Zernike polynomials. Astigmatism was the predominant surface aberration in anterior and posterior lens surfaces (accounting for ~55% and ~63% of the variance respectively), followed by spherical terms, coma, trefoil and tetrafoil. The amount of anterior and posterior surface astigmatism did not vary significantly with age. The relative angle between anterior and posterior surface astigmatism axes was on average 36.5 deg, tended to decrease with age, and was >45 deg in 36.1% lenses. The anterior surface RMS spherical term, RMS coma and 3rd order RMS decreased significantly with age. In general, there was a statistically significant correlation between the 3rd and 4th order terms of the anterior and posterior surfaces. Understanding the coordination of anterior and posterior lens surface geometries and their topographical changes with age sheds light into the role of the lens in the optical properties of the eye and the lens aging mechanism. PMID:25360371

  5. 3D Surface Reconstruction and Automatic Camera Calibration

    NASA Technical Reports Server (NTRS)

    Jalobeanu, Andre

    2004-01-01

    Illustrations in this view-graph presentation are presented on a Bayesian approach to 3D surface reconstruction and camera calibration.Existing methods, surface analysis and modeling,preliminary surface reconstruction results, and potential applications are addressed.

  6. VIRTOPSY--scientific documentation, reconstruction and animation in forensic: individual and real 3D data based geo-metric approach including optical body/object surface and radiological CT/MRI scanning.

    PubMed

    Thali, Michael J; Braun, Marcel; Buck, Ursula; Aghayev, Emin; Jackowski, Christian; Vock, Peter; Sonnenschein, Martin; Dirnhofer, Richard

    2005-03-01

    Until today, most of the documentation of forensic relevant medical findings is limited to traditional 2D photography, 2D conventional radiographs, sketches and verbal description. There are still some limitations of the classic documentation in forensic science especially if a 3D documentation is necessary. The goal of this paper is to demonstrate new 3D real data based geo-metric technology approaches. This paper present approaches to a 3D geo-metric documentation of injuries on the body surface and internal injuries in the living and deceased cases. Using modern imaging methods such as photogrammetry, optical surface and radiological CT/MRI scanning in combination it could be demonstrated that a real, full 3D data based individual documentation of the body surface and internal structures is possible in a non-invasive and non-destructive manner. Using the data merging/fusing and animation possibilities, it is possible to answer reconstructive questions of the dynamic development of patterned injuries (morphologic imprints) and to evaluate the possibility, that they are matchable or linkable to suspected injury-causing instruments. For the first time, to our knowledge, the method of optical and radiological 3D scanning was used to document the forensic relevant injuries of human body in combination with vehicle damages. By this complementary documentation approach, individual forensic real data based analysis and animation were possible linking body injuries to vehicle deformations or damages. These data allow conclusions to be drawn for automobile accident research, optimization of vehicle safety (pedestrian and passenger) and for further development of crash dummies. Real 3D data based documentation opens a new horizon for scientific reconstruction and animation by bringing added value and a real quality improvement in forensic science.

  7. Optical 3D imaging and visualization of concealed objects

    NASA Astrophysics Data System (ADS)

    Berginc, G.; Bellet, J.-B.; Berechet, I.; Berechet, S.

    2016-09-01

    This paper gives new insights on optical 3D imagery. In this paper we explore the advantages of laser imagery to form a three-dimensional image of the scene. 3D laser imaging can be used for three-dimensional medical imaging and surveillance because of ability to identify tumors or concealed objects. We consider the problem of 3D reconstruction based upon 2D angle-dependent laser images. The objective of this new 3D laser imaging is to provide users a complete 3D reconstruction of objects from available 2D data limited in number. The 2D laser data used in this paper come from simulations that are based on the calculation of the laser interactions with the different meshed objects of the scene of interest or from experimental 2D laser images. We show that combining the Radom transform on 2D laser images with the Maximum Intensity Projection can generate 3D views of the considered scene from which we can extract the 3D concealed object in real time. With different original numerical or experimental examples, we investigate the effects of the input contrasts. We show the robustness and the stability of the method. We have developed a new patented method of 3D laser imaging based on three-dimensional reflective tomographic reconstruction algorithms and an associated visualization method. In this paper we present the global 3D reconstruction and visualization procedures.

  8. Research of 3D display using anamorphic optics

    NASA Astrophysics Data System (ADS)

    Matsumoto, Kenji; Honda, Toshio

    1997-05-01

    This paper describes the auto-stereoscopic display which can reconstruct more reality and viewer friendly 3-D image by increasing the number of parallaxes and giving motion parallax horizontally. It is difficult to increase number of parallaxes to give motion parallax to the 3-D image without reducing the resolution, because the resolution of display device is insufficient. The magnification and the image formation position can be selected independently in horizontal direction and the vertical direction by projecting between the display device and the 3-D image with the anamorphic optics. The anamorphic optics is an optics system with different magnification in horizontal direction and the vertical direction. It consists of the combination of cylindrical lenses with different focal length. By using this optics, even if we use a dynamic display such as liquid crystal display (LCD), it is possible to display the realistic 3-D image having motion parallax. Motion parallax is obtained by assuming width of the single parallax at the viewing position to be about the same size as the pupil diameter of viewer. In addition, because the focus depth of the 3-D image is deep in this method, conflict of accommodation and convergence is small, and natural 3-D image can be displayed.

  9. Test target for characterizing 3D resolution of optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Hu, Zhixiong; Hao, Bingtao; Liu, Wenli; Hong, Baoyu; Li, Jiao

    2014-12-01

    Optical coherence tomography (OCT) is a non-invasive 3D imaging technology which has been applied or investigated in many diagnostic fields including ophthalmology, dermatology, dentistry, cardiovasology, endoscopy, brain imaging and so on. Optical resolution is an important characteristic that can describe the quality and utility of an image acquiring system. We employ 3D printing technology to design and fabricate a test target for characterizing 3D resolution of optical coherence tomography. The test target which mimics USAF 1951 test chart was produced with photopolymer. By measuring the 3D test target, axial resolution as well as lateral resolution of a spectral domain OCT system was evaluated. For comparison, conventional microscope and surface profiler were employed to characterize the 3D test targets. The results demonstrate that the 3D resolution test targets have the potential of qualitatively and quantitatively validating the performance of OCT systems.

  10. 3D simulation for solitons used in optical fibers

    NASA Astrophysics Data System (ADS)

    Vasile, F.; Tebeica, C. M.; Schiopu, P.; Vladescu, M.

    2016-12-01

    In this paper is described 3D simulation for solitions used in optical fibers. In the scientific works is started from nonlinear propagation equation and the solitons represents its solutions. This paper presents the simulation of the fundamental soliton in 3D together with simulation of the second order soliton in 3D. These simulations help in the study of the optical fibers for long distances and in the interactions between the solitons. This study helps the understanding of the nonlinear propagation equation and for nonlinear waves. These 3D simulations are obtained using MATLAB programming language, and we can observe fundamental difference between the soliton and the second order/higher order soliton and in their evolution.

  11. Programmable Bidirectional Folding of Metallic Thin Films for 3D Chiral Optical Antennas.

    PubMed

    Mao, Yifei; Zheng, Yun; Li, Can; Guo, Lin; Pan, Yini; Zhu, Rui; Xu, Jun; Zhang, Weihua; Wu, Wengang

    2017-03-10

    3D structures with characteristic lengths ranging from nanometer to micrometer scale often exhibit extraordinary optical properties, and have been becoming an extensively explored field for building new generation nanophotonic devices. Albeit a few methods have been developed for fabricating 3D optical structures, constructing 3D structures with nanometer accuracy, diversified materials, and perfect morphology is an extremely challenging task. This study presents a general 3D nanofabrication technique, the focused ion beam stress induced deformation process, which allows a programmable and accurate bidirectional folding (-70°-+90°) of various metal and dielectric thin films. Using this method, 3D helical optical antennas with different handedness, improved surface smoothness, and tunable geometries are fabricated, and the strong optical rotation effects of single helical antennas are demonstrated.

  12. Investigation of surface wave amplitudes in 3-D velocity and 3-D Q models

    NASA Astrophysics Data System (ADS)

    Ruan, Y.; Zhou, Y.

    2010-12-01

    It has been long recognized that seismic amplitudes depend on both wave speed structures and anelasticity (Q) structures. However, the effects of lateral heterogeneities in wave speed and Q structures on seismic amplitudes has not been well understood. We investigate the effects of 3-D wave speed and 3-D anelasticity (Q) structures on surface-wave amplitudes based upon wave propagation simulations of twelve globally-distributed earthquakes and 801 stations in Earth models with and without lateral heterogeneities in wave speed and anelasticity using a Spectral Element Method (SEM). Our tomographic-like 3-D Q models are converted from a velocity model S20RTS using a set of reasonable mineralogical parameters, assuming lateral perturbations in both velocity and Q are due to temperature perturbations. Surface-wave amplitude variations of SEM seismograms are measured in the period range of 50--200 s using boxcar taper, cosine taper and Slepian multi-tapers. We calculate ray-theoretical predictions of surface-wave amplitude perturbations due to elastic focusing, attenuation, and anelastic focusing which respectively depend upon the second spatial derivative (''roughness'') of perturbations in phase velocity, 1/Q, and the roughness of perturbations in 1/Q. Both numerical experiments and theoretical calculations show that (1) for short-period (~ 50 s) surface waves, the effects of amplitude attenuation due to 3-D Q structures are comparable with elastic focusing effects due to 3-D wave speed structures; and (2) for long-period (> 100 s) surface waves, the effects of attenuation become much weaker than elastic focusing; and (3) elastic focusing effects are correlated with anelastic focusing at all periods due to the correlation between velocity and Q models; and (4) amplitude perturbations are depend on measurement techniques and therefore cannot be directly compared with ray-theoretical predictions because ray theory does not account for the effects of measurement

  13. Progresses in 3D integral imaging with optical processing

    NASA Astrophysics Data System (ADS)

    Martínez-Corral, Manuel; Martínez-Cuenca, Raúl; Saavedra, Genaro; Navarro, Héctor; Pons, Amparo; Javidi, Bahram

    2008-11-01

    Integral imaging is a promising technique for the acquisition and auto-stereoscopic display of 3D scenes with full parallax and without the need of any additional devices like special glasses. First suggested by Lippmann in the beginning of the 20th century, integral imaging is based in the intersection of ray cones emitted by a collection of 2D elemental images which store the 3D information of the scene. This paper is devoted to the study, from the ray optics point of view, of the optical effects and interaction with the observer of integral imaging systems.

  14. 3D surface and body documentation in forensic medicine: 3-D/CAD Photogrammetry merged with 3D radiological scanning.

    PubMed

    Thali, Michael J; Braun, Marcel; Wirth, Joachim; Vock, Peter; Dirnhofer, Richard

    2003-11-01

    A main goal of forensic medicine is to document and to translate medical findings to a language and/or visualization that is readable and understandable for judicial persons and for medical laymen. Therefore, in addition to classical methods, scientific cutting-edge technologies can and should be used. Through the use of the Forensic, 3-D/CAD-supported Photogrammetric method the documentation of so-called "morphologic fingerprints" has been realized. Forensic, 3-D/CAD-supported Photogrammetry creates morphologic data models of the injury and of the suspected injury-causing instrument allowing the evaluation of a match between the injury and the instrument. In addition to the photogrammetric body surface registration, the radiological documentation provided by a volume scan (i.e., spiral, multi-detector CT, or MRI) registers the sub-surface injury, which is not visible to Photogrammetry. The new, combined method of merging Photogrammetry and Radiology data sets creates the potential to perform many kinds of reconstructions and postprocessing of (patterned) injuries in the realm of forensic medical case work. Using this merging method of colored photogrammetric surface and gray-scale radiological internal documentation, a great step towards a new kind of reality-based, high-tech wound documentation and visualization in forensic medicine is made. The combination of the methods of 3D/CAD Photogrammetry and Radiology has the advantage of being observer-independent, non-subjective, non-invasive, digitally storable over years or decades and even transferable over the web for second opinion.

  15. Optical Approach to Resin Formulation for 3D Printed Microfluidics†

    PubMed Central

    Gong, Hua; Beauchamp, Michael; Perry, Steven; Woolley, Adam T.

    2015-01-01

    Microfluidics imposes different requirements on 3D printing compared to many applications because the critical features for microfluidics consist of internal microvoids. Resins for general 3D printing applications, however, are not necessarily formulated to meet the requirements of microfluidics and minimize the size of fabricated voids. In this paper we use an optical approach to guide custom formulation of resins to minimize the cross sectional size of fabricated flow channels as exemplars of such voids. We focus on stereolithgraphy (SL) 3D printing with Digital Light Processing (DLP) based on a micromirror array and use a commercially available 3D printer. We develop a mathematical model for the optical dose delivered through the thickness of a 3D printed part, including the effect of voids. We find that there is a fundamental trade-off between the homogeneity of the optical dose within individual layers and how far the critical dose penetrates into a flow channel during fabrication. We also experimentally investigate the practical limits of flow channel miniaturization given the optical properties of a resin and find that the minimum flow channel height is ~3.5–5.5ha where ha is the optical penetration depth of the resin, and that the minimum width is 4 pixels in the build plane. We also show that the ratio of the build layer thickness to ha should be in the range 0.3–1.0 to obtain the minimum flow channel height for a given resin. The minimum flow channel size that we demonstrate for a custom resin is 60 μm × 108 μm for a 10 μm build layer thickness. This work lays the foundation for 3D printing of <100 μm microfluidic features. PMID:26744624

  16. 3D optical measuring technologies for dimensional inspection

    NASA Astrophysics Data System (ADS)

    Chugui, Yu V.

    2005-01-01

    The results of the R & D activity of TDI SIE SB RAS in the field of the 3D optical measuring technologies and systems for noncontact 3D optical dimensional inspection applied to atomic and railway industry safety problems are presented. This activity includes investigations of diffraction phenomena on some 3D objects, using the original constructive calculation method, development of hole inspection method on the base of diffractive optical elements. Ensuring the safety of nuclear reactors and running trains as well as their high exploitation reliability takes a noncontact inspection of geometrical parameters of their components. For this tasks we have developed methods and produced the technical vision measuring systems LMM, CONTROL, PROFILE, and technologies for non-contact 3D dimensional inspection of grid spacers and fuel elements for the nuclear reactor VVER-1000 and VVER-440, as well as automatic laser diagnostic system COMPLEX for noncontact inspection of geometrical parameters of running freight car wheel pairs. The performances of these systems and the results of the industrial testing at atomic and railway companies are presented.

  17. 3D optical measuring technologies and systems for industrial applications

    NASA Astrophysics Data System (ADS)

    Chugui, Yu. V.

    2005-06-01

    The results of the R & D activity of TDI SIE SB RAS in the field of the 3D optical measuring technologies and systems for noncontact 3D optical dimensional inspection applied to atomic and railway industry safety problems are presented. This activity includes investigations of diffraction phenomena on some 3D objects, using the original constructive calculation method, development of hole inspection method on the base of diffractive optical elements. Ensuring the safety of nuclear reactors and running trains as well as their high exploitation reliability requires a 100 % noncontact precise inspection of geometrical parameters of their components. To solve this problem we have developed methods and produced the technical vision measuring systems LMM, CONTROL, RADAR, and technologies for noncontact 3D dimensional inspection of grid spacers and fuel elements for the nuclear reactor VVER-1000 and VVER-440, as well as automatic laser diagnostic COMPLEX for noncontact inspection of geometric parameters of running freight car wheel pairs. The performances of these systems and the results of industrial testing are presented and discussed. The created devices are in pilot operation at Atomic and Railway Companies.

  18. Unity Occupation of Sites in a 3D Optical Lattice

    NASA Astrophysics Data System (ADS)

    Depue, Marshall T.; McCormick, Colin; Winoto, S. Lukman; Oliver, Steven; Weiss, David S.

    1999-03-01

    An average filling factor of one atom per lattice site has been obtained in a submicron scale far-off-resonance optical lattice (FORL). High site occupation is obtained through a compression sequence that includes laser cooling in a 3D FORL and adiabatic toggling between the 3D FORL and a 1D FORL trap. After the highest filling factor is achieved, laser cooling causes collisional loss from lattice sites with more than one atom. Ultimately 44% of the sites have a single atom cooled to near its vibrational ground state. A theoretical model of site occupation based on Poisson statistics agrees well with the experimental results.

  19. From Surface Data to 3D Geologic Maps

    NASA Astrophysics Data System (ADS)

    Dhont, D.; Luxey, P.; Longuesserre, V.; Monod, B.; Guillaume, B.

    2008-12-01

    New trends in earth sciences are mostly related to technologies allowing graphical representations of the geology in 3D. However, the concept of 3D geologic map is commonly misused. For instance, displays of geologic maps draped onto DEM in rotating perspective views have been misleadingly called 3D geologic maps, but this still cannot provide any volumetric underground information as a true 3D geologic map should. Here, we present a way to produce mathematically and geometrically correct 3D geologic maps constituted by the volume and shape of all geologic features of a given area. The originality of the method is that it is based on the integration of surface data only consisting of (1) geologic maps, (2) satellite images, (3) DEM and (4) bedding dips and strikes. To generate 3D geologic maps, we used a 3D geologic modeler that combines and extrapolates the surface information into a coherent 3D data set. The significance of geometrically correct 3D geologic maps is demonstrated for various geologic settings and applications. 3D models are of primarily importance for educational purposes because they reveal features that standard 2D geologic maps by themselves could not show. The 3D visualization helps in the understanding of the geometrical relationship between the different geologic features and, in turn, for the quantification of the geology at the regional scale. Furthermore, given the logistical challenges associated with modern oil and mineral exploration in remote and rugged terrain, these volume-based models can provide geological and commercial insight prior to seismic evaluation.

  20. 3D SEM for surface topography quantification - a case study on dental surfaces

    NASA Astrophysics Data System (ADS)

    Glon, F.; Flys, O.; Lööf, P.-J.; Rosén, B.-G.

    2014-03-01

    3D analysis of surface topography is becoming a more used tool for industry and research. New ISO standards are being launched to assist in quantifying engineering surfaces. The traditional optical measuring instrumentation used for 3D surface characterization has been optical interferometers and confocal based instrumentation. However, the resolution here is limited in the lateral dimension to the wavelength of visible light to about 500 nm. The great advantage using the SEM for topography measurements is the high flexibility to zoom from low magnifications and locating interesting areas to high magnification of down to nanometer large surface features within seconds. This paper presents surface characterization of dental implant micro topography. 3D topography data was created from SEM images using commercial photogrammetric software. A coherence scanning interferometer was used for reference measurements to compare with the 3D SEM measurements on relocated areas. As a result of this study, measurements emphasizes that the correlation between the accepted CSI measurements and the new technology represented by photogrammetry based on SEM images for many areal characterization parameters are around or less than 20%. The importance of selecting sampling and parameter sensitivity to varying sampling is high-lighted. Future work includes a broader study of limitations of the photogrammetry technique on certified micro-geometries and more application surfaces at different scales.

  1. High speed 3D surface inspection with digital holography

    NASA Astrophysics Data System (ADS)

    Brunn, Andreas; Aspert, Nicolas; Cuche, Etienne; Emery, Yves; Ettemeyer, Andreas

    2013-01-01

    Digital holography has proven its ability to acquire high accuracy full field 3D data with one single image acquisition. This means that in principle this technique offers the chance to perform 3D serial inspection processes, as well. However, one limitation in digital holography is its limited ability to measure rough surfaces. In the presence of rough surfaces, the magnification of the image has to be increased to capture the required phase information on each camera pixel. However, this leads to significant reduction of inspection speed. If low magnification is selected, the rough surface produces speckles which cannot be treated properly by digital holography algorithms. In this paper, we describe the extension of digital holography to rough surface applications using speckle interferometry technique. This technique is capable of fast inspection of rough surfaces with sub-micrometer accuracy. The principle of this approach is shown and a practical application for 3D surface inspection of wafer cutting processes is given.

  2. Optical Sensors and Methods for Underwater 3D Reconstruction

    PubMed Central

    Massot-Campos, Miquel; Oliver-Codina, Gabriel

    2015-01-01

    This paper presents a survey on optical sensors and methods for 3D reconstruction in underwater environments. The techniques to obtain range data have been listed and explained, together with the different sensor hardware that makes them possible. The literature has been reviewed, and a classification has been proposed for the existing solutions. New developments, commercial solutions and previous reviews in this topic have also been gathered and considered. PMID:26694389

  3. Open-source 3D-printable optics equipment.

    PubMed

    Zhang, Chenlong; Anzalone, Nicholas C; Faria, Rodrigo P; Pearce, Joshua M

    2013-01-01

    Just as the power of the open-source design paradigm has driven down the cost of software to the point that it is accessible to most people, the rise of open-source hardware is poised to drive down the cost of doing experimental science to expand access to everyone. To assist in this aim, this paper introduces a library of open-source 3-D-printable optics components. This library operates as a flexible, low-cost public-domain tool set for developing both research and teaching optics hardware. First, the use of parametric open-source designs using an open-source computer aided design package is described to customize the optics hardware for any application. Second, details are provided on the use of open-source 3-D printers (additive layer manufacturing) to fabricate the primary mechanical components, which are then combined to construct complex optics-related devices. Third, the use of the open-source electronics prototyping platform are illustrated as control for optical experimental apparatuses. This study demonstrates an open-source optical library, which significantly reduces the costs associated with much optical equipment, while also enabling relatively easily adapted customizable designs. The cost reductions in general are over 97%, with some components representing only 1% of the current commercial investment for optical products of similar function. The results of this study make its clear that this method of scientific hardware development enables a much broader audience to participate in optical experimentation both as research and teaching platforms than previous proprietary methods.

  4. Open-Source 3D-Printable Optics Equipment

    PubMed Central

    Zhang, Chenlong; Anzalone, Nicholas C.; Faria, Rodrigo P.; Pearce, Joshua M.

    2013-01-01

    Just as the power of the open-source design paradigm has driven down the cost of software to the point that it is accessible to most people, the rise of open-source hardware is poised to drive down the cost of doing experimental science to expand access to everyone. To assist in this aim, this paper introduces a library of open-source 3-D-printable optics components. This library operates as a flexible, low-cost public-domain tool set for developing both research and teaching optics hardware. First, the use of parametric open-source designs using an open-source computer aided design package is described to customize the optics hardware for any application. Second, details are provided on the use of open-source 3-D printers (additive layer manufacturing) to fabricate the primary mechanical components, which are then combined to construct complex optics-related devices. Third, the use of the open-source electronics prototyping platform are illustrated as control for optical experimental apparatuses. This study demonstrates an open-source optical library, which significantly reduces the costs associated with much optical equipment, while also enabling relatively easily adapted customizable designs. The cost reductions in general are over 97%, with some components representing only 1% of the current commercial investment for optical products of similar function. The results of this study make its clear that this method of scientific hardware development enables a much broader audience to participate in optical experimentation both as research and teaching platforms than previous proprietary methods. PMID:23544104

  5. Large optical 3D MEMS switches in access networks

    NASA Astrophysics Data System (ADS)

    Madamopoulos, Nicholas; Kaman, Volkan; Yuan, Shifu; Jerphagnon, Olivier; Helkey, Roger; Bowers, John E.

    2007-09-01

    Interest is high among residential customers and businesses for advanced, broadband services such as fast Internet access, electronic commerce, video-on-demand, digital broadcasting, teleconferencing and telemedicine. In order to satisfy such growing demand of end-customers, access technologies such as fiber-to-the-home/building (FTTH/B) are increasingly being deployed. Carriers can reduce maintenance costs, minimize technology obsolescence and introduce new services easily by reducing active elements in the fiber access network. However, having a passive optical network (PON) also introduces operational and maintenance challenges. Increased diagnostic monitoring capability of the network becomes a necessity as more and more fibers are provisioned to deliver services to the end-customers. This paper demonstrates the clear advantages that large 3D optical MEMS switches offer in solving these access network problems. The advantages in preventative maintenance, remote monitoring, test and diagnostic capability are highlighted. The low optical insertion loss for all switch optical connections of the switch enables the monitoring, grooming and serving of a large number of PON lines and customers. Furthermore, the 3D MEMS switch is transparent to optical wavelengths and data formats, thus making it easy to incorporate future upgrades, such higher bit rates or DWDM overlay to a PON.

  6. Studies of the 3D surface roughness height

    SciTech Connect

    Avisane, Anita; Rudzitis, Janis; Kumermanis, Maris

    2013-12-16

    Nowadays nano-coatings occupy more and more significant place in technology. Innovative, functional coatings acquire new aspects from the point of view of modern technologies, considering the aggregate of physical properties that can be achieved manipulating in the production process with the properties of coatings’ surfaces on micro- and nano-level. Nano-coatings are applied on machine parts, friction surfaces, contacting parts, corrosion surfaces, transparent conducting films (TCF), etc. The equipment available at present for the production of transparent conducting oxide (TCO) coatings with highest quality is based on expensive indium tin oxide (ITO) material; therefore cheaper alternatives are being searched for. One such offered alternative is zink oxide (ZnO) nano-coatings. Evaluating the TCF physical and mechanical properties and in view of the new ISO standard (EN ISO 25178) on the introduction of surface texture (3D surface roughness) in the engineering calculations, it is necessary to examine the height of 3D surface roughness, which is one of the most significant roughness parameters. The given paper studies the average values of 3D surface roughness height and the most often applied distribution laws are as follows: the normal distribution and Rayleigh distribution. The 3D surface is simulated by a normal random field.

  7. Constructing 3D microtubule networks using holographic optical trapping

    PubMed Central

    Bergman, J.; Osunbayo, O.; Vershinin, M.

    2015-01-01

    Developing abilities to assemble nanoscale structures is a major scientific and engineering challenge. We report a technique which allows precise positioning and manipulation of individual rigid filaments, enabling construction of custom-designed 3D filament networks. This approach uses holographic optical trapping (HOT) for nano-positioning and microtubules (MTs) as network building blocks. MTs are desirable engineering components due to their high aspect ratio, rigidity, and their ability to serve as substrate for directed nano-transport, reflecting their roles in the eukaryotic cytoskeleton. The 3D architecture of MT cytoskeleton is a significant component of its function, however experimental tools to study the roles of this geometric complexity in a controlled environment have been lacking. We demonstrate the broad capabilities of our system by building a self-supporting 3D MT-based nanostructure and by conducting a MT-based transport experiment on a dynamically adjustable 3D MT intersection. Our methodology not only will advance studies of cytoskeletal networks (and associated processes such as MT-based transport) but will also likely find use in engineering nanostructures and devices. PMID:26657337

  8. Multimodal 3D cancer-mimicking optical phantom

    PubMed Central

    Smith, Gennifer T.; Lurie, Kristen L.; Zlatev, Dimitar V.; Liao, Joseph C.; Ellerbee Bowden, Audrey K.

    2016-01-01

    Three-dimensional (3D) organ-mimicking phantoms provide realistic imaging environments for testing various aspects of optical systems, including for evaluating new probe designs, characterizing the diagnostic potential of new technologies, and assessing novel image processing algorithms prior to validation in real tissue. We introduce and characterize the use of a new material, Dragon Skin (Smooth-On Inc.), and fabrication technique, air-brushing, for fabrication of a 3D phantom that mimics the appearance of a real organ under multiple imaging modalities. We demonstrate the utility of the material and technique by fabricating the first 3D, hollow bladder phantom with realistic normal and multi-stage pathology features suitable for endoscopic detection using the gold standard imaging technique, white light cystoscopy (WLC), as well as the complementary imaging modalities of optical coherence tomography and blue light cystoscopy, which are aimed at improving the sensitivity and specificity of WLC to bladder cancer detection. The flexibility of the material and technique used for phantom construction allowed for the representation of a wide range of diseased tissue states, ranging from inflammation (benign) to high-grade cancerous lesions. Such phantoms can serve as important tools for trainee education and evaluation of new endoscopic instrumentation. PMID:26977369

  9. Optic flow aided navigation and 3D scene reconstruction

    NASA Astrophysics Data System (ADS)

    Rollason, Malcolm

    2013-10-01

    An important enabler for low cost airborne systems is the ability to exploit low cost inertial instruments. An Inertial Navigation System (INS) can provide a navigation solution, when GPS is denied, by integrating measurements from inertial sensors. However, the gyrometer and accelerometer biases of low cost inertial sensors cause compound errors in the integrated navigation solution. This paper describes experiments to establish whether (and to what extent) the navigation solution can be aided by fusing measurements from an on-board video camera with measurements from the inertial sensors. The primary aim of the work was to establish whether optic flow aided navigation is beneficial even when the 3D structure within the observed scene is unknown. A further aim was to investigate whether an INS can help to infer 3D scene content from video. Experiments with both real and synthetic data have been conducted. Real data was collected using an AR Parrot quadrotor. Empirical results illustrate that optic flow provides a useful aid to navigation even when the 3D structure of the observed scene is not known. With optic flow aiding of the INS, the computed trajectory is consistent with the true camera motion, whereas the unaided INS yields a rapidly increasing position error (the data represents ~40 seconds, after which the unaided INS is ~50 metres in error and has passed through the ground). The results of the Monte Carlo simulation concur with the empirical result. Position errors, which grow as a quadratic function of time when unaided, are substantially checked by the availability of optic flow measurements.

  10. 3D high resolution pure optical photoacoustic microscopy

    NASA Astrophysics Data System (ADS)

    Xie, Zhixing; Chen, Sung-Liang; Ling, Tao; Guo, L. Jay; Carson, Paul L.; Wang, Xueding

    2012-02-01

    The concept of pure optical photoacoustic microscopy(POPAM) was proposed based on optical rastering of a focused excitation beam and optically sensing the photoacoustic signal using a microring resonator fabricated by a nanoimprinting technique. After some refinedment of in the resonator structure and mold fabrication, an ultrahigh Q factor of 3.0×105 was achieved which provided high sensitivity with a noise equivalent detectable pressure(NEDP) value of 29Pa. This NEDP is much lower than the hundreds of Pascals achieved with existing optical resonant structures such as etalons, fiber gratings and dielectric multilayer interference filters available for acoustic measurement. The featured high sensitivity allowed the microring resonator to detect the weak photoacoustic signals from micro- or submicroscale objects. The inherent superbroad bandwidth of the optical microring resonator combined with an optically focused scanning beam provided POPAM of high resolution in the axial as well as both lateral directions while the axial resolution of conventional photoacoustic microscopy (PAM) suffers from the limited bandwidth of PZT detectors. Furthermore, the broadband microring resonator showed similar sensitivity to that of our most sensitive PZT detector. The current POPAM system provides a lateral resolution of 5μm and an axial resolution of 8μm, comparable to that achieved by optical microscopy while presenting the unique contrast of optical absorption and functional information complementing other optical modalities. The 3D structure of microvasculature, including capillary networks, and even individual red blood cells have been discerned successfully in the proof-of-concept experiments on mouse bladders ex vivo and mouse ears in vivo. The potential of approximately GHz bandwidth of the microring resonator also might allow much higher resolution than shown here in microscopy of optical absorption and acoustic propagation properties at depths in unfrozen tissue

  11. Beam Optics Analysis - An Advanced 3D Trajectory Code

    SciTech Connect

    Ives, R. Lawrence; Bui, Thuc; Vogler, William; Neilson, Jeff; Read, Mike; Shephard, Mark; Bauer, Andrew; Datta, Dibyendu; Beal, Mark

    2006-01-03

    Calabazas Creek Research, Inc. has completed initial development of an advanced, 3D program for modeling electron trajectories in electromagnetic fields. The code is being used to design complex guns and collectors. Beam Optics Analysis (BOA) is a fully relativistic, charged particle code using adaptive, finite element meshing. Geometrical input is imported from CAD programs generating ACIS-formatted files. Parametric data is inputted using an intuitive, graphical user interface (GUI), which also provides control of convergence, accuracy, and post processing. The program includes a magnetic field solver, and magnetic information can be imported from Maxwell 2D/3D and other programs. The program supports thermionic emission and injected beams. Secondary electron emission is also supported, including multiple generations. Work on field emission is in progress as well as implementation of computer optimization of both the geometry and operating parameters. The principle features of the program and its capabilities are presented.

  12. Optical characterization of different types of 3D displays

    NASA Astrophysics Data System (ADS)

    Boher, Pierre; Leroux, Thierry; Bignon, Thibault; Collomb-Patton, Véronique

    2012-03-01

    All 3D displays have the same intrinsic method to induce depth perception. They provide different images in the left and right eye of the observer to obtain the stereoscopic effect. The three most common solutions already available on the market are active glass, passive glass and auto-stereoscopic 3D displays. The three types of displays are based on different physical principle (polarization, time selection or spatial emission) and consequently require different measurement instruments and techniques. In the proposed paper, we present some of these solutions and the technical characteristics that can be obtained to compare the displays. We show in particular that local and global measurements can be made in the three cases to access to different characteristics. We also discuss the new technologies currently under development and their needs in terms of optical characterization.

  13. Tracking tissue section surfaces for automated 3D confocal cytometry

    NASA Astrophysics Data System (ADS)

    Agustin, Ramses; Price, Jeffrey H.

    2002-05-01

    Three-dimensional cytometry, whereby large volumes of tissue would be measured automatically, requires a computerized method for detecting the upper and lower tissue boundaries. In conventional confocal microscopy, the user interactively sets limits for axial scanning for each field-of-view. Biological specimens vary in section thickness, thereby driving the requirement for setting vertical scan limits. Limits could be set arbitrarily large to ensure the entire tissue is scanned, but automatic surface identification would eliminate storing undue numbers of empty optical sections and forms the basis for incorporating lateral microscope stage motion to collect unlimited numbers of stacks. This walk-away automation of 3D confocal scanning for biological imaging is the first sep towards practical, computerized statistical sampling from arbitrarily large tissue volumes. Preliminary results for automatic tissue surface tracking were obtained for phase-contrast microscopy by measuring focus sharpness (previously used for high-speed autofocus by our group). Measurements were taken from 5X5 fields-of-view from hamster liver sections, varying from five to twenty microns in thickness, then smoothed to lessen variations of in-focus information at each axial position. Because image sharpness (as the power of high spatial frequency components) drops across the axial boundaries of a tissue section, mathematical quantities including the full-width at half-maximum, extrema in the first derivative, and second derivative were used to locate the proximal and distal surfaces of a tissue. Results from these tests were evaluated against manual (i.e., visual) determination of section boundaries.

  14. Structured light imaging system for structural and optical characterization of 3D tissue-simulating phantoms

    NASA Astrophysics Data System (ADS)

    Liu, Songde; Smith, Zach; Xu, Ronald X.

    2016-10-01

    There is a pressing need for a phantom standard to calibrate medical optical devices. However, 3D printing of tissue-simulating phantom standard is challenged by lacking of appropriate methods to characterize and reproduce surface topography and optical properties accurately. We have developed a structured light imaging system to characterize surface topography and optical properties (absorption coefficient and reduced scattering coefficient) of 3D tissue-simulating phantoms. The system consisted of a hyperspectral light source, a digital light projector (DLP), a CMOS camera, two polarizers, a rotational stage, a translation stage, a motion controller, and a personal computer. Tissue-simulating phantoms with different structural and optical properties were characterized by the proposed imaging system and validated by a standard integrating sphere system. The experimental results showed that the proposed system was able to achieve pixel-level optical properties with a percentage error of less than 11% for absorption coefficient and less than 7% for reduced scattering coefficient for phantoms without surface curvature. In the meanwhile, 3D topographic profile of the phantom can be effectively reconstructed with an accuracy of less than 1% deviation error. Our study demonstrated that the proposed structured light imaging system has the potential to characterize structural profile and optical properties of 3D tissue-simulating phantoms.

  15. A 3D surface imaging system for assessing human obesity

    NASA Astrophysics Data System (ADS)

    Xu, B.; Yu, W.; Yao, M.; Yao, X.; Li, Q.; Pepper, M. R.; Freeland-Graves, J. H.

    2009-08-01

    The increasing prevalence of obesity suggests a need to develop a convenient, reliable and economical tool for assessment of this condition. Three-dimensional (3D) body surface imaging has emerged as an exciting technology for estimation of body composition. This paper presents a new 3D body imaging system, which was designed for enhanced portability, affordability, and functionality. In this system, stereo vision technology was used to satisfy the requirements for a simple hardware setup and fast image acquisitions. The portability of the system was created via a two-stand configuration, and the accuracy of body volume measurements was improved by customizing stereo matching and surface reconstruction algorithms that target specific problems in 3D body imaging. Body measurement functions dedicated to body composition assessment also were developed. The overall performance of the system was evaluated in human subjects by comparison to other conventional anthropometric methods, as well as air displacement plethysmography, for body fat assessment.

  16. Innovations in 3D printing: a 3D overview from optics to organs.

    PubMed

    Schubert, Carl; van Langeveld, Mark C; Donoso, Larry A

    2014-02-01

    3D printing is a method of manufacturing in which materials, such as plastic or metal, are deposited onto one another in layers to produce a three dimensional object, such as a pair of eye glasses or other 3D objects. This process contrasts with traditional ink-based printers which produce a two dimensional object (ink on paper). To date, 3D printing has primarily been used in engineering to create engineering prototypes. However, recent advances in printing materials have now enabled 3D printers to make objects that are comparable with traditionally manufactured items. In contrast with conventional printers, 3D printing has the potential to enable mass customisation of goods on a large scale and has relevance in medicine including ophthalmology. 3D printing has already been proved viable in several medical applications including the manufacture of eyeglasses, custom prosthetic devices and dental implants. In this review, we discuss the potential for 3D printing to revolutionise manufacturing in the same way as the printing press revolutionised conventional printing. The applications and limitations of 3D printing are discussed; the production process is demonstrated by producing a set of eyeglass frames from 3D blueprints.

  17. Performance assessment of 3D surface imaging technique for medical imaging applications

    NASA Astrophysics Data System (ADS)

    Li, Tuotuo; Geng, Jason; Li, Shidong

    2013-03-01

    Recent development in optical 3D surface imaging technologies provide better ways to digitalize the 3D surface and its motion in real-time. The non-invasive 3D surface imaging approach has great potential for many medical imaging applications, such as motion monitoring of radiotherapy, pre/post evaluation of plastic surgery and dermatology, to name a few. Various commercial 3D surface imaging systems have appeared on the market with different dimension, speed and accuracy. For clinical applications, the accuracy, reproducibility and robustness across the widely heterogeneous skin color, tone, texture, shape properties, and ambient lighting is very crucial. Till now, a systematic approach for evaluating the performance of different 3D surface imaging systems still yet exist. In this paper, we present a systematic performance assessment approach to 3D surface imaging system assessment for medical applications. We use this assessment approach to exam a new real-time surface imaging system we developed, dubbed "Neo3D Camera", for image-guided radiotherapy (IGRT). The assessments include accuracy, field of view, coverage, repeatability, speed and sensitivity to environment, texture and color.

  18. Ultracold Heteronuclear Molecules in a 3D Optical Lattice

    SciTech Connect

    Ospelkaus, C.; Ospelkaus, S.; Humbert, L.; Ernst, P.; Sengstock, K.; Bongs, K.

    2006-09-22

    We report on the creation of ultracold heteronuclear molecules assembled from fermionic {sup 40}K and bosonic {sup 87}Rb atoms in a 3D optical lattice. Molecules are produced at a heteronuclear Feshbach resonance on both the attractive and the repulsive sides of the resonance. We precisely determine the binding energy of the heteronuclear molecules from rf spectroscopy across the Feshbach resonance. We characterize the lifetime of the molecular sample as a function of magnetic field and measure lifetimes between 20 and 120 ms. The efficiency of molecule creation via rf association is measured and is found to decrease as expected for more deeply bound molecules.

  19. 3D refractive index measurements of special optical fibers

    NASA Astrophysics Data System (ADS)

    Yan, Cheng; Huang, Su-Juan; Miao, Zhuang; Chang, Zheng; Zeng, Jun-Zhang; Wang, Ting-Yun

    2016-09-01

    A digital holographic microscopic chromatography-based approach with considerably improved accuracy, simplified configuration and performance stability is proposed to measure three dimensional refractive index of special optical fibers. Based on the approach, a measurement system is established incorporating a modified Mach-Zehnder interferometer and lab-developed supporting software for data processing. In the system, a phase projection distribution of an optical fiber is utilized to obtain an optimal digital hologram recorded by a CCD, and then an angular spectrum theory-based algorithm is adopted to extract the phase distribution information of an object wave. The rotation of the optic fiber enables the experimental measurements of multi-angle phase information. Based on the filtered back projection algorithm, a 3D refraction index of the optical fiber is thus obtained at high accuracy. To evaluate the proposed approach, both PANDA fibers and special elliptical optical fiber are considered in the system. The results measured in PANDA fibers agree well with those measured using S14 Refractive Index Profiler, which is, however, not suitable for measuring the property of a special elliptical fiber.

  20. Surface classification and detection of latent fingerprints based on 3D surface texture parameters

    NASA Astrophysics Data System (ADS)

    Gruhn, Stefan; Fischer, Robert; Vielhauer, Claus

    2012-06-01

    In the field of latent fingerprint detection in crime scene forensics the classification of surfaces has importance. A new method for the scientific analysis of image based information for forensic science was investigated in the last years. Our image acquisition based on a sensor using Chromatic White Light (CWL) with a lateral resolution up to 2 μm. The used FRT-MicroProf 200 CWL 600 measurement device is able to capture high-resolution intensity and topography images in an optical and contact-less way. In prior work, we have suggested to use 2D surface texture parameters to classify various materials, which was a novel approach in the field of criminalistic forensic using knowledge from surface appearance and a chromatic white light sensor. A meaningful and useful classification of different crime scene specific surfaces is not existent. In this work, we want to extend such considerations by the usage of fourteen 3D surface parameters, called 'Birmingham 14'. In our experiment we define these surface texture parameters and use them to classify ten different materials in this test set-up and create specific material classes. Further it is shown in first experiments, that some surface texture parameters are sensitive to separate fingerprints from carrier surfaces. So far, the use of surface roughness is mainly known within the framework of material quality control. The analysis and classification of the captured 3D-topography images from crime scenes is important for the adaptive preprocessing depending on the surface texture. The adaptive preprocessing in dependency of surface classification is necessary for precise detection because of the wide variety of surface textures. We perform a preliminary study in usage of these 3D surface texture parameters as feature for the fingerprint detection. In combination with a reference sample we show that surface texture parameters can be an indication for a fingerprint and can be a feature in latent fingerprint detection.

  1. Synthesizing 3D Surfaces from Parameterized Strip Charts

    NASA Technical Reports Server (NTRS)

    Robinson, Peter I.; Gomez, Julian; Morehouse, Michael; Gawdiak, Yuri

    2004-01-01

    We believe 3D information visualization has the power to unlock new levels of productivity in the monitoring and control of complex processes. Our goal is to provide visual methods to allow for rapid human insight into systems consisting of thousands to millions of parameters. We explore this hypothesis in two complex domains: NASA program management and NASA International Space Station (ISS) spacecraft computer operations. We seek to extend a common form of visualization called the strip chart from 2D to 3D. A strip chart can display the time series progression of a parameter and allows for trends and events to be identified. Strip charts can be overlayed when multiple parameters need to visualized in order to correlate their events. When many parameters are involved, the direct overlaying of strip charts can become confusing and may not fully utilize the graphing area to convey the relationships between the parameters. We provide a solution to this problem by generating 3D surfaces from parameterized strip charts. The 3D surface utilizes significantly more screen area to illustrate the differences in the parameters and the overlayed strip charts, and it can rapidly be scanned by humans to gain insight. The selection of the third dimension must be a parallel or parameterized homogenous resource in the target domain, defined using a finite, ordered, enumerated type, and not a heterogeneous type. We demonstrate our concepts with examples from the NASA program management domain (assessing the state of many plans) and the computers of the ISS (assessing the state of many computers). We identify 2D strip charts in each domain and show how to construct the corresponding 3D surfaces. The user can navigate the surface, zooming in on regions of interest, setting a mark and drilling down to source documents from which the data points have been derived. We close by discussing design issues, related work, and implementation challenges.

  2. 3D surface configuration modulates 2D symmetry detection.

    PubMed

    Chen, Chien-Chung; Sio, Lok-Teng

    2015-02-01

    We investigated whether three-dimensional (3D) information in a scene can affect symmetry detection. The stimuli were random dot patterns with 15% dot density. We measured the coherence threshold, or the proportion of dots that were the mirror reflection of the other dots in the other half of the image about a central vertical axis, at 75% accuracy with a 2AFC paradigm under various 3D configurations produced by the disparity between the left and right eye images. The results showed that symmetry detection was difficult when the corresponding dots across the symmetry axis were on different frontoparallel or inclined planes. However, this effect was not due to a difference in distance, as the observers could detect symmetry on a slanted surface, where the depth of the two sides of the symmetric axis was different. The threshold was reduced for a hinge configuration where the join of two slanted surfaces coincided with the axis of symmetry. Our result suggests that the detection of two-dimensional (2D) symmetry patterns is subject to the 3D configuration of the scene; and that coplanarity across the symmetry axis and consistency between the 2D pattern and 3D structure are important factors for symmetry detection.

  3. Objective breast symmetry evaluation using 3-D surface imaging.

    PubMed

    Eder, Maximilian; Waldenfels, Fee V; Swobodnik, Alexandra; Klöppel, Markus; Pape, Ann-Kathrin; Schuster, Tibor; Raith, Stefan; Kitzler, Elena; Papadopulos, Nikolaos A; Machens, Hans-Günther; Kovacs, Laszlo

    2012-04-01

    This study develops an objective breast symmetry evaluation using 3-D surface imaging (Konica-Minolta V910(®) scanner) by superimposing the mirrored left breast over the right and objectively determining the mean 3-D contour difference between the 2 breast surfaces. 3 observers analyzed the evaluation protocol precision using 2 dummy models (n = 60), 10 test subjects (n = 300), clinically tested it on 30 patients (n = 900) and compared it to established 2-D measurements on 23 breast reconstructive patients using the BCCT.core software (n = 690). Mean 3-D evaluation precision, expressed as the coefficient of variation (VC), was 3.54 ± 0.18 for all human subjects without significant intra- and inter-observer differences (p > 0.05). The 3-D breast symmetry evaluation is observer independent, significantly more precise (p < 0.001) than the BCCT.core software (VC = 6.92 ± 0.88) and may play a part in an objective surgical outcome analysis after incorporation into clinical practice.

  4. Quantitative 3D Optical Imaging: Applications in Dosimetry and Biophysics

    NASA Astrophysics Data System (ADS)

    Thomas, Andrew Stephen

    Optical-CT has been shown to be a potentially useful imaging tool for the two very different spheres of biologists and radiation therapy physicists, but it has yet to live up to that potential. In radiation therapy, researchers have used optical-CT for the readout of 3D dosimeters, but it is yet to be a clinically relevant tool as the technology is too slow to be considered practical. Biologists have used the technique for structural imaging, but have struggled with emission tomography as the reality of photon attenuation for both excitation and emission have made the images quantitatively irrelevant. Dosimetry. The DLOS (Duke Large field of view Optical-CT Scanner) was designed and constructed to make 3D dosimetry utilizing optical-CT a fast and practical tool while maintaining the accuracy of readout of the previous, slower readout technologies. Upon construction/optimization/implementation of several components including a diffuser, band pass filter, registration mount & fluid filtration system the dosimetry system provides high quality data comparable to or exceeding that of commercial products. In addition, a stray light correction algorithm was tested and implemented. The DLOS in combination with the 3D dosimeter it was designed for, PREAGETM, then underwent rigorous commissioning and benchmarking tests validating its performance against gold standard data including a set of 6 irradiations. DLOS commissioning tests resulted in sub-mm isotropic spatial resolution (MTF >0.5 for frequencies of 1.5lp/mm) and a dynamic range of ˜60dB. Flood field uniformity was 10% and stable after 45minutes. Stray light proved to be small, due to telecentricity, but even the residual can be removed through deconvolution. Benchmarking tests showed the mean 3D passing gamma rate (3%, 3mm, 5% dose threshold) over the 6 benchmark data sets was 97.3% +/- 0.6% (range 96%-98%) scans totaling ˜10 minutes, indicating excellent ability to perform 3D dosimetry while improving the speed of

  5. Satellite and Surface Data Synergy for Developing a 3D Cloud Structure and Properties Characterization Over the ARM SGP. Stage 1: Cloud Amounts, Optical Depths, and Cloud Heights Reconciliation

    NASA Technical Reports Server (NTRS)

    Genkova, I.; Long, C. N.; Heck, P. W.; Minnis, P.

    2003-01-01

    One of the primary Atmospheric Radiation Measurement (ARM) Program objectives is to obtain measurements applicable to the development of models for better understanding of radiative processes in the atmosphere. We address this goal by building a three-dimensional (3D) characterization of the cloud structure and properties over the ARM Southern Great Plains (SGP). We take the approach of juxtaposing the cloud properties as retrieved from independent satellite and ground-based retrievals, and looking at the statistics of the cloud field properties. Once these retrievals are well understood, they will be used to populate the 3D characterization database. As a first step we determine the relationship between surface fractional sky cover and satellite viewing angle dependent cloud fraction (CF). We elaborate on the agreement intercomparing optical depth (OD) datasets from satellite and ground using available retrieval algorithms with relation to the CF, cloud height, multi-layer cloud presence, and solar zenith angle (SZA). For the SGP Central Facility, where output from the active remote sensing cloud layer (ARSCL) valueadded product (VAP) is available, we study the uncertainty of satellite estimated cloud heights and evaluate the impact of this uncertainty for radiative studies.

  6. 3D mapping of elastic modulus using shear wave optical micro-elastography

    NASA Astrophysics Data System (ADS)

    Zhu, Jiang; Qi, Li; Miao, Yusi; Ma, Teng; Dai, Cuixia; Qu, Yueqiao; He, Youmin; Gao, Yiwei; Zhou, Qifa; Chen, Zhongping

    2016-10-01

    Elastography provides a powerful tool for histopathological identification and clinical diagnosis based on information from tissue stiffness. Benefiting from high resolution, three-dimensional (3D), and noninvasive optical coherence tomography (OCT), optical micro-elastography has the ability to determine elastic properties with a resolution of ~10 μm in a 3D specimen. The shear wave velocity measurement can be used to quantify the elastic modulus. However, in current methods, shear waves are measured near the surface with an interference of surface waves. In this study, we developed acoustic radiation force (ARF) orthogonal excitation optical coherence elastography (ARFOE-OCE) to visualize shear waves in 3D. This method uses acoustic force perpendicular to the OCT beam to excite shear waves in internal specimens and uses Doppler variance method to visualize shear wave propagation in 3D. The measured propagation of shear waves agrees well with the simulation results obtained from finite element analysis (FEA). Orthogonal acoustic excitation allows this method to measure the shear modulus in a deeper specimen which extends the elasticity measurement range beyond the OCT imaging depth. The results show that the ARFOE-OCE system has the ability to noninvasively determine the 3D elastic map.

  7. 3D mapping of elastic modulus using shear wave optical micro-elastography

    PubMed Central

    Zhu, Jiang; Qi, Li; Miao, Yusi; Ma, Teng; Dai, Cuixia; Qu, Yueqiao; He, Youmin; Gao, Yiwei; Zhou, Qifa; Chen, Zhongping

    2016-01-01

    Elastography provides a powerful tool for histopathological identification and clinical diagnosis based on information from tissue stiffness. Benefiting from high resolution, three-dimensional (3D), and noninvasive optical coherence tomography (OCT), optical micro-elastography has the ability to determine elastic properties with a resolution of ~10 μm in a 3D specimen. The shear wave velocity measurement can be used to quantify the elastic modulus. However, in current methods, shear waves are measured near the surface with an interference of surface waves. In this study, we developed acoustic radiation force (ARF) orthogonal excitation optical coherence elastography (ARFOE-OCE) to visualize shear waves in 3D. This method uses acoustic force perpendicular to the OCT beam to excite shear waves in internal specimens and uses Doppler variance method to visualize shear wave propagation in 3D. The measured propagation of shear waves agrees well with the simulation results obtained from finite element analysis (FEA). Orthogonal acoustic excitation allows this method to measure the shear modulus in a deeper specimen which extends the elasticity measurement range beyond the OCT imaging depth. The results show that the ARFOE-OCE system has the ability to noninvasively determine the 3D elastic map. PMID:27762276

  8. Characterisation of the surface structure of 3D printed scaffolds for cell infiltration and surgical suturing.

    PubMed

    Ruiz-Cantu, Laura; Gleadall, Andrew; Faris, Callum; Segal, Joel; Shakesheff, Kevin; Yang, Jing

    2016-03-01

    3D printing is of great interest for tissue engineering scaffolds due to the ability to form complex geometries and control internal structures, including porosity and pore size. The porous structure of scaffolds plays an important role in cell ingrowth and nutrition infusion. Although the internal porosity and pore size of 3D printed scaffolds have been frequently studied, the surface porosity and pore size, which are critical for cell infiltration and mass transport, have not been investigated. The surface geometry can differ considerably from the internal scaffold structure depending on the 3D printing process. It is vital to be able to control the surface geometry of scaffolds as well as the internal structure to fabricate optimal architectures. This work presents a method to control the surface porosity and pore size of 3D printed scaffolds. Six scaffold designs have been printed with surface porosities ranging from 3% to 21%. We have characterised the overall scaffold porosity and surface porosity using optical microscopy and microCT. It has been found that surface porosity has a significant impact on cell infiltration and proliferation. In addition, the porosity of the surface has been found to have an effect on mechanical properties and on the forces required to penetrate the scaffold with a surgical suturing needle. To the authors' knowledge, this study is the first to investigate the surface geometry of extrusion-based 3D printed scaffolds and demonstrates the importance of surface geometry in cell infiltration and clinical manipulation.

  9. Molecular cartography of the human skin surface in 3D.

    PubMed

    Bouslimani, Amina; Porto, Carla; Rath, Christopher M; Wang, Mingxun; Guo, Yurong; Gonzalez, Antonio; Berg-Lyon, Donna; Ackermann, Gail; Moeller Christensen, Gitte Julie; Nakatsuji, Teruaki; Zhang, Lingjuan; Borkowski, Andrew W; Meehan, Michael J; Dorrestein, Kathleen; Gallo, Richard L; Bandeira, Nuno; Knight, Rob; Alexandrov, Theodore; Dorrestein, Pieter C

    2015-04-28

    The human skin is an organ with a surface area of 1.5-2 m(2) that provides our interface with the environment. The molecular composition of this organ is derived from host cells, microbiota, and external molecules. The chemical makeup of the skin surface is largely undefined. Here we advance the technologies needed to explore the topographical distribution of skin molecules, using 3D mapping of mass spectrometry data and microbial 16S rRNA amplicon sequences. Our 3D maps reveal that the molecular composition of skin has diverse distributions and that the composition is defined not only by skin cells and microbes but also by our daily routines, including the application of hygiene products. The technological development of these maps lays a foundation for studying the spatial relationships of human skin with hygiene, the microbiota, and environment, with potential for developing predictive models of skin phenotypes tailored to individual health.

  10. Molecular cartography of the human skin surface in 3D

    PubMed Central

    Bouslimani, Amina; Porto, Carla; Rath, Christopher M.; Wang, Mingxun; Guo, Yurong; Gonzalez, Antonio; Berg-Lyon, Donna; Ackermann, Gail; Moeller Christensen, Gitte Julie; Nakatsuji, Teruaki; Zhang, Lingjuan; Borkowski, Andrew W.; Meehan, Michael J.; Dorrestein, Kathleen; Gallo, Richard L.; Bandeira, Nuno; Knight, Rob; Alexandrov, Theodore; Dorrestein, Pieter C.

    2015-01-01

    The human skin is an organ with a surface area of 1.5–2 m2 that provides our interface with the environment. The molecular composition of this organ is derived from host cells, microbiota, and external molecules. The chemical makeup of the skin surface is largely undefined. Here we advance the technologies needed to explore the topographical distribution of skin molecules, using 3D mapping of mass spectrometry data and microbial 16S rRNA amplicon sequences. Our 3D maps reveal that the molecular composition of skin has diverse distributions and that the composition is defined not only by skin cells and microbes but also by our daily routines, including the application of hygiene products. The technological development of these maps lays a foundation for studying the spatial relationships of human skin with hygiene, the microbiota, and environment, with potential for developing predictive models of skin phenotypes tailored to individual health. PMID:25825778

  11. Ultracold polar molecules in a 3D optical lattice

    NASA Astrophysics Data System (ADS)

    Yan, Bo

    2015-05-01

    Ultracold polar molecules, with their long-range electric dipolar interactions, offer new opportunities for studying quantum magnetism and many-body physics. KRb molecules loaded into a three-dimensional (3D) optical lattice allow one to study such a spin-lattice system in a stable environment without losses arising from chemical reactions. In the case with strong lattice confinement along two directions and a weak lattice potential along the third, we find the loss rate is suppressed by the quantum Zeno effect. In a deep 3D lattice with no tunneling, we observe evidences for spin exchange interactions. We use Ramsey spectroscopy to investigate the spin dynamics. By choosing the appropriate lattice polarizations and implementing a spin echo sequence, the single particle dephasing is largely suppressed, leaving the dipolar exchange interactions as the dominant contribution to the observed dynamics. This is supported by many-body theoretical calculations. While this initial demonstration was done with low lattice fillings, our current experimental efforts are focused on increasing the lattice filling fraction. This will greatly benefit the study of complex many-body dynamics with long-range interactions, such as transport of excitations in an out-of-equilibrium system and spin-orbit coupling in a lattice.

  12. Real-time 3D surface-image-guided beam setup in radiotherapy of breast cancer

    SciTech Connect

    Djajaputra, David; Li Shidong

    2005-01-01

    We describe an approach for external beam radiotherapy of breast cancer that utilizes the three-dimensional (3D) surface information of the breast. The surface data of the breast are obtained from a 3D optical camera that is rigidly mounted on the ceiling of the treatment vault. This 3D camera utilizes light in the visible range therefore it introduces no ionization radiation to the patient. In addition to the surface topographical information of the treated area, the camera also captures gray-scale information that is overlaid on the 3D surface image. This allows us to visualize the skin markers and automatically determine the isocenter position and the beam angles in the breast tangential fields. The field sizes and shapes of the tangential, supraclavicular, and internal mammary gland fields can all be determined according to the 3D surface image of the target. A least-squares method is first introduced for the tangential-field setup that is useful for compensation of the target shape changes. The entire process of capturing the 3D surface data and subsequent calculation of beam parameters typically requires less than 1 min. Our tests on phantom experiments and patient images have achieved the accuracy of 1 mm in shift and 0.5 deg. in rotation. Importantly, the target shape and position changes in each treatment session can both be corrected through this real-time image-guided system.

  13. Parsing optical scanned 3D data by Bayesian inference

    NASA Astrophysics Data System (ADS)

    Xiong, Hanwei; Xu, Jun; Xu, Chenxi; Pan, Ming

    2015-10-01

    Optical devices are always used to digitize complex objects to get their shapes in form of point clouds. The results have no semantic meaning about the objects, and tedious process is indispensable to segment the scanned data to get meanings. The reason for a person to perceive an object correctly is the usage of knowledge, so Bayesian inference is used to the goal. A probabilistic And-Or-Graph is used as a unified framework of representation, learning, and recognition for a large number of object categories, and a probabilistic model defined on this And-Or-Graph is learned from a relatively small training set per category. Given a set of 3D scanned data, the Bayesian inference constructs a most probable interpretation of the object, and a semantic segment is obtained from the part decomposition. Some examples are given to explain the method.

  14. Design Application Translates 2-D Graphics to 3-D Surfaces

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Fabric Images Inc., specializing in the printing and manufacturing of fabric tension architecture for the retail, museum, and exhibit/tradeshow communities, designed software to translate 2-D graphics for 3-D surfaces prior to print production. Fabric Images' fabric-flattening design process models a 3-D surface based on computer-aided design (CAD) specifications. The surface geometry of the model is used to form a 2-D template, similar to a flattening process developed by NASA's Glenn Research Center. This template or pattern is then applied in the development of a 2-D graphic layout. Benefits of this process include 11.5 percent time savings per project, less material wasted, and the ability to improve upon graphic techniques and offer new design services. Partners include Exhibitgroup/Giltspur (end-user client: TAC Air, a division of Truman Arnold Companies Inc.), Jack Morton Worldwide (end-user client: Nickelodeon), as well as 3D Exhibits Inc., and MG Design Associates Corp.

  15. Study of 3D printing method for GRIN micro-optics devices

    NASA Astrophysics Data System (ADS)

    Wang, P. J.; Yeh, J. A.; Hsu, W. Y.; Cheng, Y. C.; Lee, W.; Wu, N. H.; Wu, C. Y.

    2016-03-01

    Conventional optical elements are based on either refractive or reflective optics theory to fulfill the design specifications via optics performance data. In refractive optical lenses, the refractive index of materials and radius of curvature of element surfaces determine the optical power and wavefront aberrations so that optical performance can be further optimized iteratively. Although gradient index (GRIN) phenomenon in optical materials is well studied for more than a half century, the optics theory in lens design via GRIN materials is still yet to be comprehensively investigated before realistic GRIN lenses are manufactured. In this paper, 3D printing method for manufacture of micro-optics devices with special features has been studied based on methods reported in the literatures. Due to the additive nature of the method, GRIN lenses in micro-optics devices seem to be readily achievable if a design methodology is available. First, derivation of ray-tracing formulae is introduced for all possible structures in GRIN lenses. Optics simulation program is employed for characterization of GRIN lenses with performance data given by aberration coefficients in Zernike polynomial. Finally, a proposed structure of 3D printing machine is described with conceptual illustration.

  16. Recursive estimation of 3D motion and surface structure from local affine flow parameters.

    PubMed

    Calway, Andrew

    2005-04-01

    A recursive structure from motion algorithm based on optical flow measurements taken from an image sequence is described. It provides estimates of surface normals in addition to 3D motion and depth. The measurements are affine motion parameters which approximate the local flow fields associated with near-planar surface patches in the scene. These are integrated over time to give estimates of the 3D parameters using an extended Kalman filter. This also estimates the camera focal length and, so, the 3D estimates are metric. The use of parametric measurements means that the algorithm is computationally less demanding than previous optical flow approaches and the recursive filter builds in a degree of noise robustness. Results of experiments on synthetic and real image sequences demonstrate that the algorithm performs well.

  17. Investigation Into the Accuracy of 3D Surface Roughness Characteristics

    NASA Astrophysics Data System (ADS)

    Kumermanis, M.; Rudzitis, J.; Mozga, N.; Ancans, A.; Grislis, A.

    2014-04-01

    The existing standards for surface roughness cover only two dimensions, while in reality this is three-dimensional (3D). In particular, the 3D surface roughness parameters are important for solving the contact surface mechanics problems as related to the accuracy of 3D surface roughness characteristics. One of the most important factors for determination of 3D characteristics is the number of data points (NDP) on the x- and y-axes (i.e. in cut-off length). The NDP has a profound effect on the accuracy of measurement results, measuring time and volume of the output data (especially along the y-axis, where the NDP is identical to the number of parallel profiles). At a too small NDP the results will be incorrect and with too broad scatter, while a too large NDP - though not enlarging the range of basic information - considerably increases the measuring time. Therefore, the aim of the work was to find the optimal NDP for such surface processing methods as grinding, spark erosion and shot methods of surface treatment. Eksistējošie virsmas raupjuma standarti apskata virsmas raupjumu tikai divās dimensijās. Tomēr reālais virsmas raupjums pēc savas dabas ir trīsdimensiju (3D) objekts. Līdz ar to virsmas raupjums ir jāraksturo ar 3D parametriem. Un no šo parametru noteikšanas precizitātes ir atkarīgi tālākie virsmas aprēķini, piemēram, virsmu kontaktēšanās process. Viens no svarīgākajiem faktoriem, raksturojot virsmas raupjumu 3D, pielietojot kontakta tipa mēriekārtas, ir datu punktu skaits pa abām mērīšanas asīm x un y. Ar datu punktu skaitu mēs saprotam to skaitu mērīšanas bāzes garumā. Datu punktu skaits būtiski ietekmē sagaidāmo mērījumu rezultātu precizitāti, mērīšanai nepieciešamo laiku un izejas datu faila izmērus (sevišķi y-ass virzienā, kur katrs datu punkts ir paralēls profils). Datu punktu skaitam ir jābūt optimālam. Pārāk mazs punktu skaits noved pie neprecīziem rezultātiem un lielas to izkliedes, savuk

  18. Design of a 3-D surface scanner for lower limb prosthetics: a technical note.

    PubMed

    Commean, P K; Smith, K E; Vannier, M W

    1996-07-01

    A three-dimensional (3-D) noncontact optical surface range sensing imaging system that captures the entire circumferential and distal end surfaces of lower limb residua in less than 1 second has been developed. The optical surface scanner (OSS) consists of four charge injection device (CID) cameras and three white light projectors, mounted on a rigid frame surrounding the subject's residuum, allowing 360 degrees surface coverage of the lower residual limb. Anatomic 3-D computer graphics reconstruction of a residuum surface, recorded with the OSS imaging system, is used for visualization and measurement. One cubical and two spherical calibration test objects were used to obtain a system precision of less than 1 mm. In a study conducted with 13 persons with below knee (BK) amputation, the OSS system was compared to calipers, electromagnetic digitizer, and volumetric computed tomography with better than 1 mm precision on plaster positive casts and approximately 2 mm on the residual limbs.

  19. Visualizing 3D velocity fields near contour surfaces

    SciTech Connect

    Max, N.; Crawfis, R.; Grant, C.

    1994-03-01

    Vector field rendering is difficult in 3D because the vector icons overlap and hide each other. We propose four different techniques for visualizing vector fields only near surfaces. The first uses motion blurred particles in a thickened region around the surface. The second uses a voxel grid to contain integral curves of the vector field. The third uses many antialiased lines through the surface, and the fourth uses hairs sprouting from the surface and then bending in the direction of the vector field. All the methods use the graphite pipeline, allowing real time rotation and interaction, and the first two methods can animate the texture to move in the flow determined by the velocity field.

  20. 3D Surface Topology Guides Stem Cell Adhesion and Differentiation

    PubMed Central

    Viswanathan, Priyalakshmi; Ondeck, Matthew G.; Chirasatitsin, Somyot; Nghamkham, Kamolchanok; Reilly, Gwendolen C.; Engler, Adam J.; Battaglia, Giuseppe

    2015-01-01

    Polymerized high internal phase emulsion (polyHIPE) foams are extremely versatile materials for investigating cell-substrate interactions in vitro. Foam morphologies can be controlled by polymerization conditions to result in either open or closed pore structures with different levels of connectivity, consequently enabling the comparison between 2D and 3D matrices using the same substrate with identical surface chemistry conditions. Additionally, here we achieve the control of pore surface topology (i.e. how different ligands are clustered together) using amphiphilic block copolymers as emulsion stabilisers. We demonstrate that adhesion of human mesenchymal progenitor (hES-MP) cells cultured on polyHIPE foams is dependent on foam surface topology and chemistry but is independent of porosity and interconnectivity. We also demonstrate that the interconnectivity, architecture and surface topology of the foams has an effect on the osteogenic differentiation potential of hES-MP cells. Together these data demonstrate that the adhesive heterogeneity of a 3D scaffold could regulate not only mesenchymal stem cell attachment but also cell behavior in the absence of soluble growth factors. PMID:25818420

  1. Effective 3-D surface modeling for geographic information systems

    NASA Astrophysics Data System (ADS)

    Yüksek, K.; Alparslan, M.; Mendi, E.

    2016-01-01

    In this work, we propose a dynamic, flexible and interactive urban digital terrain platform with spatial data and query processing capabilities of geographic information systems, multimedia database functionality and graphical modeling infrastructure. A new data element, called Geo-Node, which stores image, spatial data and 3-D CAD objects is developed using an efficient data structure. The system effectively handles data transfer of Geo-Nodes between main memory and secondary storage with an optimized directional replacement policy (DRP) based buffer management scheme. Polyhedron structures are used in digital surface modeling and smoothing process is performed by interpolation. The experimental results show that our framework achieves high performance and works effectively with urban scenes independent from the amount of spatial data and image size. The proposed platform may contribute to the development of various applications such as Web GIS systems based on 3-D graphics standards (e.g., X3-D and VRML) and services which integrate multi-dimensional spatial information and satellite/aerial imagery.

  2. A 3D integral imaging optical see-through head-mounted display.

    PubMed

    Hua, Hong; Javidi, Bahram

    2014-06-02

    An optical see-through head-mounted display (OST-HMD), which enables optical superposition of digital information onto the direct view of the physical world and maintains see-through vision to the real world, is a vital component in an augmented reality (AR) system. A key limitation of the state-of-the-art OST-HMD technology is the well-known accommodation-convergence mismatch problem caused by the fact that the image source in most of the existing AR displays is a 2D flat surface located at a fixed distance from the eye. In this paper, we present an innovative approach to OST-HMD designs by combining the recent advancement of freeform optical technology and microscopic integral imaging (micro-InI) method. A micro-InI unit creates a 3D image source for HMD viewing optics, instead of a typical 2D display surface, by reconstructing a miniature 3D scene from a large number of perspective images of the scene. By taking advantage of the emerging freeform optical technology, our approach will result in compact, lightweight, goggle-style AR display that is potentially less vulnerable to the accommodation-convergence discrepancy problem and visual fatigue. A proof-of-concept prototype system is demonstrated, which offers a goggle-like compact form factor, non-obstructive see-through field of view, and true 3D virtual display.

  3. Optical 3D sensor for large objects in industrial application

    NASA Astrophysics Data System (ADS)

    Kuhmstedt, Peter; Heinze, Matthias; Himmelreich, Michael; Brauer-Burchardt, Christian; Brakhage, Peter; Notni, Gunther

    2005-06-01

    A new self calibrating optical 3D measurement system using fringe projection technique named "kolibri 1500" is presented. It can be utilised to acquire the all around shape of large objects. The basic measuring principle is the phasogrammetric approach introduced by the authors /1, 2/. The "kolibri 1500" consists of a stationary system with a translation unit for handling of objects. Automatic whole body measurement is achieved by using sensor head rotation and changeable object position, which can be done completely computer controlled. Multi-view measurement is realised by using the concept of virtual reference points. In this way no matching procedures or markers are necessary for the registration of the different images. This makes the system very flexible to realise different measurement tasks. Furthermore, due to self calibrating principle mechanical alterations are compensated. Typical parameters of the system are: the measurement volume extends from 400 mm up to 1500 mm max. length, the measurement time is between 2 min for 12 images up to 20 min for 36 images and the measurement accuracy is below 50μm.The flexibility makes the measurement system useful for a wide range of applications such as quality control, rapid prototyping, design and CAD/CAM which will be shown in the paper.

  4. Cordless hand-held optical 3D sensor

    NASA Astrophysics Data System (ADS)

    Munkelt, Christoph; Bräuer-Burchardt, Christian; Kühmstedt, Peter; Schmidt, Ingo; Notni, Gunther

    2007-07-01

    A new mobile optical 3D measurement system using phase correlation based fringe projection technique will be presented. The sensor consist of a digital projection unit and two cameras in a stereo arrangement, whereby both are battery powered. The data transfer to a base station will be done via WLAN. This gives the possibility to use the system in complicate, remote measurement situations, which are typical in archaeology and architecture. In the measurement procedure the sensor will be hand-held by the user, illuminating the object with a sequence of less than 10 fringe patterns, within a time below 200 ms. This short sequence duration was achieved by a new approach, which combines the epipolar constraint with robust phase correlation utilizing a pre-calibrated sensor head, containing two cameras and a digital fringe projector. Furthermore, the system can be utilized to acquire the all around shape of objects by using the phasogrammetric approach with virtual land marks introduced by the authors 1, 2. This way no matching procedures or markers are necessary for the registration of multiple views, which makes the system very flexible in accomplishing different measurement tasks. The realized measurement field is approx. 100 mm up to 400 mm in diameter. The mobile character makes the measurement system useful for a wide range of applications in arts, architecture, archaeology and criminology, which will be shown in the paper.

  5. Depth propagation and surface construction in 3-D vision.

    PubMed

    Georgeson, Mark A; Yates, Tim A; Schofield, Andrew J

    2009-01-01

    In stereo vision, regions with ambiguous or unspecified disparity can acquire perceived depth from unambiguous regions. This has been called stereo capture, depth interpolation or surface completion. We studied some striking induced depth effects suggesting that depth interpolation and surface completion are distinct stages of visual processing. An inducing texture (2-D Gaussian noise) had sinusoidal modulation of disparity, creating a smooth horizontal corrugation. The central region of this surface was replaced by various test patterns whose perceived corrugation was measured. When the test image was horizontal 1-D noise, shown to one eye or to both eyes without disparity, it appeared corrugated in much the same way as the disparity-modulated (DM) flanking regions. But when the test image was 2-D noise, or vertical 1-D noise, little or no depth was induced. This suggests that horizontal orientation was a key factor. For a horizontal sine-wave luminance grating, strong depth was induced, but for a square-wave grating, depth was induced only when its edges were aligned with the peaks and troughs of the DM flanking surface. These and related results suggest that disparity (or local depth) propagates along horizontal 1-D features, and then a 3-D surface is constructed from the depth samples acquired. The shape of the constructed surface can be different from the inducer, and so surface construction appears to operate on the results of a more local depth propagation process.

  6. Conveying the 3D Shape of Transparent Surfaces Via Texture

    NASA Technical Reports Server (NTRS)

    Interrante, Victoria; Fuchs, Henry; Pizer, Stephen

    1997-01-01

    Transparency can be a useful device for depicting multiple overlapping surfaces in a single image. The challenge is to render the transparent surfaces in such a way that their three-dimensional shape can be readily understood and their depth distance from underlying structures clearly perceived. This paper describes our investigations into the use of sparsely-distributed discrete, opaque texture as an 'artistic device' for more explicitly indicating the relative depth of a transparent surface and for communicating the essential features of its 3D shape in an intuitively meaningful and minimally occluding way. The driving application for this work is the visualization of layered surfaces in radiation therapy treatment planning data, and the technique is illustrated on transparent isointensity surfaces of radiation dose. We describe the perceptual motivation and artistic inspiration for defining a stroke texture that is locally oriented in the direction of greatest normal curvature (and in which individual strokes are of a length proportional to the magnitude of the curvature in the direction they indicate), and discuss several alternative methods for applying this texture to isointensity surfaces defined in a volume. We propose an experimental paradigm for objectively measuring observers' ability to judge the shape and depth of a layered transparent surface, in the course of a task relevant to the needs of radiotherapy treatment planning, and use this paradigm to evaluate the practical effectiveness of our approach through a controlled observer experiment based on images generated from actual clinical data.

  7. Poly(acrylic acid) brushes pattern as a 3D functional biosensor surface for microchips

    NASA Astrophysics Data System (ADS)

    Wang, Yan-Mei; Cui, Yi; Cheng, Zhi-Qiang; Song, Lu-Sheng; Wang, Zhi-You; Han, Bao-Hang; Zhu, Jin-Song

    2013-02-01

    Poly(acrylic acid) (PAA) brushes, a novel three dimensional (3D) precursor layer of biosensor or protein microarrays, possess high protein loading level and low non-specific protein adsorption. In this article, we describe a simple and convenient way to fabricate 3D PAA brushes pattern by microcontact printing (μCP) and characterize it with FT-IR and optical microscopy. The carboxyl groups of PAA brushes can be applied to covalently immobilize protein for immunoassay. Thriving 3D space made by patterning PAA brushes thin film is available to enhance protein immobilization, which is confirmed by measuring model protein interaction between human immunoglobulin G (H-IgG) and goat anti-H-IgG (G-H-IgG) with fluorescence microscopy and surface plasmon resonance imaging (SPRi). As expected, the SPRi signals of H-IgG coating on 3D PAA brushes pattern and further measuring specific binding with G-H-IgG are all larger than that of 3D PAA brushes without pattern and 2D bare gold surface. We further revealed that this surface can be used for high-throughput screening and clinical diagnosis by label-free assaying of Hepatitis-B-Virus surface antibody (HBsAb) with Hepatitis-B-Virus surface antigen (HBsAg) concentration array chip. The linearity range for HBsAb assay is wider than that of conventional ELISA method.

  8. Wear Analysis of Thermal Spray Coatings on 3D Surfaces

    NASA Astrophysics Data System (ADS)

    Tillmann, W.; Luo, W.; Selvadurai, U.

    2014-01-01

    Even though the application of thermal spray coatings on complex geometries gained a greater interest in the last decade, the effect of different geometrical features on the wear behavior is still ill-defined. In this study, the wear resistance of FTC-FeCSiMn coated 3D surfaces was investigated. The wear test was carried out by means of two innovative testing procedures. The first test is a Pin-on-Tubes test where the rotating motion is realized by a lathe chuck. The specimens in the second test were fixed on the table and a robot arm operated the pin. This wear test was applied on specimens with concave or convex surfaces. The residual stresses, which were determined by means of an incremental hole-drilling method, show a dependency on the substrate geometry. The obtained stresses were put in relation to the different radii. After the wear test, a 3D-profilometer determined the wear volume and the sections of the coatings were characterized by a scanning electron microscope. The results indicate that the wear resistance is strongly influenced by the geometry of the substrate.

  9. 3D Additive Construction with Regolith for Surface Systems

    NASA Technical Reports Server (NTRS)

    Mueller, Robert P.

    2014-01-01

    Planetary surface exploration on Asteroids, the Moon, Mars and Martian Moons will require the stabilization of loose, fine, dusty regolith to avoid the effects of vertical lander rocket plume impingement, to keep abrasive and harmful dust from getting lofted and for dust free operations. In addition, the same regolith stabilization process can be used for 3 Dimensional ( 3D) printing, additive construction techniques by repeating the 2D stabilization in many vertical layers. This will allow in-situ construction with regolith so that materials will not have to be transported from Earth. Recent work in the NASA Kennedy Space Center (KSC) Surface Systems Office (NE-S) Swamp Works and at the University of Southern California (USC) under two NASA Innovative Advanced Concept (NIAC) awards have shown promising results with regolith (crushed basalt rock) materials for in-situ heat shields, bricks, landing/launch pads, berms, roads, and other structures that could be fabricated using regolith that is sintered or mixed with a polymer binder. The technical goals and objectives of this project are to prove the feasibility of 3D printing additive construction using planetary regolith simulants and to show that they have structural integrity and practical applications in space exploration.

  10. Axial-Stereo 3-D Optical Metrology for Inner Profile of Pipes Using a Scanning Laser Endoscope.

    PubMed

    Gong, Yuanzheng; Johnston, Richard S; Melville, C David; Seibel, Eric J

    As the rapid progress in the development of optoelectronic components and computational power, 3D optical metrology becomes more and more popular in manufacturing and quality control due to its flexibility and high speed. However, most of the optical metrology methods are limited to external surfaces. This paper proposed a new approach to measure tiny internal 3D surfaces with a scanning fiber endoscope and axial-stereo vision algorithm. A dense, accurate point cloud of internally machined threads was generated to compare with its corresponding X-ray 3D data as ground truth, and the quantification was analyzed by Iterative Closest Points algorithm.

  11. Axial-Stereo 3-D Optical Metrology for Inner Profile of Pipes Using a Scanning Laser Endoscope

    PubMed Central

    Gong, Yuanzheng; Johnston, Richard S.; Melville, C. David; Seibel, Eric J.

    2015-01-01

    As the rapid progress in the development of optoelectronic components and computational power, 3D optical metrology becomes more and more popular in manufacturing and quality control due to its flexibility and high speed. However, most of the optical metrology methods are limited to external surfaces. This paper proposed a new approach to measure tiny internal 3D surfaces with a scanning fiber endoscope and axial-stereo vision algorithm. A dense, accurate point cloud of internally machined threads was generated to compare with its corresponding X-ray 3D data as ground truth, and the quantification was analyzed by Iterative Closest Points algorithm. PMID:26640425

  12. Automating laser scanning of 3D surfaces for reverse engineering

    NASA Astrophysics Data System (ADS)

    Chan, Vincent H.; Bradley, Colin H.; Vickers, Geoffrey W.

    1997-12-01

    Application of current 3-D laser scanning systems to reverse engineering is limited by two obstacles. The meticulous guidance of the laser scanner over the surface of the object being scanned and the segmentation of the cloud data which is collected by the laser scanner. Presently, both obstacles are being manually solved. The guidance of the laser scanning sensor at the correct surface to sensor distance is dependent on operator judgement and the segmentation of the collected data is reliant on the user to manually define surface boundaries on a computer screen. By applying a 2-D CCD camera, both of these problems can be resolved. Depth information on the location of the object surface can be derived from a pair of stereo images from the CCD camera. Using this depth information, the scanner path can be automatically calculated. Segmentation of the object surface can be accomplished by employing a Kohonen neural network into the CCD image. Successful segmentation of the image is conditional on the locations selected to start neural nodes as well as the prevention of the neuron connectors from bleeding onto neighboring patches. Thus the CCD camera allows for the automatic path planning of the laser scanner as well as the segmentation of the surface into patches defined along its natural boundaries.

  13. Introductory review on `Flying Triangulation': a motion-robust optical 3D measurement principle

    NASA Astrophysics Data System (ADS)

    Ettl, Svenja

    2015-04-01

    'Flying Triangulation' (FlyTri) is a recently developed principle which allows for a motion-robust optical 3D measurement of rough surfaces. It combines a simple sensor with sophisticated algorithms: a single-shot sensor acquires 2D camera images. From each camera image, a 3D profile is generated. The series of 3D profiles generated are aligned to one another by algorithms, without relying on any external tracking device. It delivers real-time feedback of the measurement process which enables an all-around measurement of objects. The principle has great potential for small-space acquisition environments, such as the measurement of the interior of a car, and motion-sensitive measurement tasks, such as the intraoral measurement of teeth. This article gives an overview of the basic ideas and applications of FlyTri. The main challenges and their solutions are discussed. Measurement examples are also given to demonstrate the potential of the measurement principle.

  14. Subtractive 3D Printing of Optically Active Diamond Structures

    NASA Astrophysics Data System (ADS)

    Martin, Aiden A.; Toth, Milos; Aharonovich, Igor

    2014-05-01

    Controlled fabrication of semiconductor nanostructures is an essential step in engineering of high performance photonic and optoelectronic devices. Diamond in particular has recently attracted considerable attention as a promising platform for quantum technologies, photonics and high resolution sensing applications. Here we demonstrate the fabrication of optically active, functional diamond structures using gas-mediated electron beam induced etching (EBIE). The technique achieves dry chemical etching at room temperature through the dissociation of surface-adsorbed H2O molecules by energetic electrons in a water vapor environment. Parallel processing is possible by electron flood exposure and the use of an etch mask, while high resolution, mask-free, iterative editing is demonstrated by direct write etching of inclined facets of diamond microparticles. The realized structures demonstrate the potential of EBIE for the fabrication of optically active structures in diamond.

  15. Surface topography study of prepared 3D printed moulds via 3D printer for silicone elastomer based nasal prosthesis

    NASA Astrophysics Data System (ADS)

    Abdullah, Abdul Manaf; Din, Tengku Noor Daimah Tengku; Mohamad, Dasmawati; Rahim, Tuan Noraihan Azila Tuan; Akil, Hazizan Md; Rajion, Zainul Ahmad

    2016-12-01

    Conventional prosthesis fabrication is highly depends on the hand creativity of laboratory technologist. The development in 3D printing technology offers a great help in fabricating affordable and fast yet esthetically acceptable prostheses. This study was conducted to discover the potential of 3D printed moulds for indirect silicone elastomer based nasal prosthesis fabrication. Moulds were designed using computer aided design (CAD) software (Solidworks, USA) and converted into the standard tessellation language (STL) file. Three moulds with layer thickness of 0.1, 0.2 and 0.3mm were printed utilizing polymer filament based 3D printer (Makerbot Replicator 2X, Makerbot, USA). Another one mould was printed utilizing liquid resin based 3D printer (Objet 30 Scholar, Stratasys, USA) as control. The printed moulds were then used to fabricate maxillofacial silicone specimens (n=10)/mould. Surface profilometer (Surfcom Flex, Accretech, Japan), digital microscope (KH77000, Hirox, USA) and scanning electron microscope (Quanta FEG 450, Fei, USA) were used to measure the surface roughness as well as the topological properties of fabricated silicone. Statistical analysis of One-Way ANOVA was employed to compare the surface roughness of the fabricated silicone elastomer. Result obtained demonstrated significant differences in surface roughness of the fabricated silicone (p<0.01). Further post hoc analysis also revealed significant differences in silicone fabricated using different 3D printed moulds (p<0.01). A 3D printed mould was successfully prepared and characterized. With surface topography that could be enhanced, inexpensive and rapid mould fabrication techniques, polymer filament based 3D printer is potential for indirect silicone elastomer based nasal prosthesis fabrication.

  16. Three-dimensional measurement of small inner surface profiles using feature-based 3-D panoramic registration

    NASA Astrophysics Data System (ADS)

    Gong, Yuanzheng; Seibel, Eric J.

    2017-01-01

    Rapid development in the performance of sophisticated optical components, digital image sensors, and computer abilities along with decreasing costs has enabled three-dimensional (3-D) optical measurement to replace more traditional methods in manufacturing and quality control. The advantages of 3-D optical measurement, such as noncontact, high accuracy, rapid operation, and the ability for automation, are extremely valuable for inline manufacturing. However, most of the current optical approaches are eligible for exterior instead of internal surfaces of machined parts. A 3-D optical measurement approach is proposed based on machine vision for the 3-D profile measurement of tiny complex internal surfaces, such as internally threaded holes. To capture the full topographic extent (peak to valley) of threads, a side-view commercial rigid scope is used to collect images at known camera positions and orientations. A 3-D point cloud is generated with multiview stereo vision using linear motion of the test piece, which is repeated by a rotation to form additional point clouds. Registration of these point clouds into a complete reconstruction uses a proposed automated feature-based 3-D registration algorithm. The resulting 3-D reconstruction is compared with x-ray computed tomography to validate the feasibility of our proposed method for future robotically driven industrial 3-D inspection.

  17. Three-dimensional measurement of small inner surface profiles using feature-based 3-D panoramic registration

    PubMed Central

    Gong, Yuanzheng; Seibel, Eric J.

    2017-01-01

    Rapid development in the performance of sophisticated optical components, digital image sensors, and computer abilities along with decreasing costs has enabled three-dimensional (3-D) optical measurement to replace more traditional methods in manufacturing and quality control. The advantages of 3-D optical measurement, such as noncontact, high accuracy, rapid operation, and the ability for automation, are extremely valuable for inline manufacturing. However, most of the current optical approaches are eligible for exterior instead of internal surfaces of machined parts. A 3-D optical measurement approach is proposed based on machine vision for the 3-D profile measurement of tiny complex internal surfaces, such as internally threaded holes. To capture the full topographic extent (peak to valley) of threads, a side-view commercial rigid scope is used to collect images at known camera positions and orientations. A 3-D point cloud is generated with multiview stereo vision using linear motion of the test piece, which is repeated by a rotation to form additional point clouds. Registration of these point clouds into a complete reconstruction uses a proposed automated feature-based 3-D registration algorithm. The resulting 3-D reconstruction is compared with x-ray computed tomography to validate the feasibility of our proposed method for future robotically driven industrial 3-D inspection. PMID:28286351

  18. In vivo bioluminescence tomography based on multi-view projection and 3D surface reconstruction

    NASA Astrophysics Data System (ADS)

    Zhang, Shuang; Wang, Kun; Leng, Chengcai; Deng, Kexin; Hu, Yifang; Tian, Jie

    2015-03-01

    Bioluminescence tomography (BLT) is a powerful optical molecular imaging modality, which enables non-invasive realtime in vivo imaging as well as 3D quantitative analysis in preclinical studies. In order to solve the inverse problem and reconstruct inner light sources accurately, the prior structural information is commonly necessary and obtained from computed tomography or magnetic resonance imaging. This strategy requires expensive hybrid imaging system, complicated operation protocol and possible involvement of ionizing radiation. The overall robustness highly depends on the fusion accuracy between the optical and structural information. In this study we present a pure optical bioluminescence tomographic system (POBTS) and a novel BLT method based on multi-view projection acquisition and 3D surface reconstruction. The POBTS acquired a sparse set of white light surface images and bioluminescent images of a mouse. Then the white light images were applied to an approximate surface model to generate a high quality textured 3D surface reconstruction of the mouse. After that we integrated multi-view luminescent images based on the previous reconstruction, and applied an algorithm to calibrate and quantify the surface luminescent flux in 3D.Finally, the internal bioluminescence source reconstruction was achieved with this prior information. A BALB/C mouse with breast tumor of 4T1-fLuc cells mouse model were used to evaluate the performance of the new system and technique. Compared with the conventional hybrid optical-CT approach using the same inverse reconstruction method, the reconstruction accuracy of this technique was improved. The distance error between the actual and reconstructed internal source was decreased by 0.184 mm.

  19. 3D modeling to characterize lamina cribrosa surface and pore geometries using in vivo images from normal and glaucomatous eyes.

    PubMed

    Sredar, Nripun; Ivers, Kevin M; Queener, Hope M; Zouridakis, George; Porter, Jason

    2013-07-01

    En face adaptive optics scanning laser ophthalmoscope (AOSLO) images of the anterior lamina cribrosa surface (ALCS) represent a 2D projected view of a 3D laminar surface. Using spectral domain optical coherence tomography images acquired in living monkey eyes, a thin plate spline was used to model the ALCS in 3D. The 2D AOSLO images were registered and projected onto the 3D surface that was then tessellated into a triangular mesh to characterize differences in pore geometry between 2D and 3D images. Following 3D transformation of the anterior laminar surface in 11 normal eyes, mean pore area increased by 5.1 ± 2.0% with a minimal change in pore elongation (mean change = 0.0 ± 0.2%). These small changes were due to the relatively flat laminar surfaces inherent in normal eyes (mean radius of curvature = 3.0 ± 0.5 mm). The mean increase in pore area was larger following 3D transformation in 4 glaucomatous eyes (16.2 ± 6.0%) due to their more steeply curved laminar surfaces (mean radius of curvature = 1.3 ± 0.1 mm), while the change in pore elongation was comparable to that in normal eyes (-0.2 ± 2.0%). This 3D transformation and tessellation method can be used to better characterize and track 3D changes in laminar pore and surface geometries in glaucoma.

  20. Colored 3D surface reconstruction using Kinect sensor

    NASA Astrophysics Data System (ADS)

    Guo, Lian-peng; Chen, Xiang-ning; Chen, Ying; Liu, Bin

    2015-03-01

    A colored 3D surface reconstruction method which effectively fuses the information of both depth and color image using Microsoft Kinect is proposed and demonstrated by experiment. Kinect depth images are processed with the improved joint-bilateral filter based on region segmentation which efficiently combines the depth and color data to improve its quality. The registered depth data are integrated to achieve a surface reconstruction through the colored truncated signed distance fields presented in this paper. Finally, the improved ray casting for rendering full colored surface is implemented to estimate color texture of the reconstruction object. Capturing the depth and color images of a toy car, the improved joint-bilateral filter based on region segmentation is used to improve the quality of depth images and the peak signal-to-noise ratio (PSNR) is approximately 4.57 dB, which is better than 1.16 dB of the joint-bilateral filter. The colored construction results of toy car demonstrate the suitability and ability of the proposed method.

  1. Air-structured optical fiber drawn from a 3D-printed preform.

    PubMed

    Cook, Kevin; Canning, John; Leon-Saval, Sergio; Reid, Zane; Hossain, Md Arafat; Comatti, Jade-Edouard; Luo, Yanhua; Peng, Gang-Ding

    2015-09-01

    A structured optical fiber is drawn from a 3D-printed structured preform. Preforms containing a single ring of holes around the core are fabricated using filament made from a modified butadiene polymer. More broadly, 3D printers capable of processing soft glasses, silica, and other materials are likely to come on line in the not-so-distant future. 3D printing of optical preforms signals a new milestone in optical fiber manufacture.

  2. Optical 3D watermark based digital image watermarking for telemedicine

    NASA Astrophysics Data System (ADS)

    Li, Xiao Wei; Kim, Seok Tae

    2013-12-01

    Region of interest (ROI) of a medical image is an area including important diagnostic information and must be stored without any distortion. This algorithm for application of watermarking technique for non-ROI of the medical image preserving ROI. The paper presents a 3D watermark based medical image watermarking scheme. In this paper, a 3D watermark object is first decomposed into 2D elemental image array (EIA) by a lenslet array, and then the 2D elemental image array data is embedded into the host image. The watermark extraction process is an inverse process of embedding. The extracted EIA through the computational integral imaging reconstruction (CIIR) technique, the 3D watermark can be reconstructed. Because the EIA is composed of a number of elemental images possesses their own perspectives of a 3D watermark object. Even though the embedded watermark data badly damaged, the 3D virtual watermark can be successfully reconstructed. Furthermore, using CAT with various rule number parameters, it is possible to get many channels for embedding. So our method can recover the weak point having only one transform plane in traditional watermarking methods. The effectiveness of the proposed watermarking scheme is demonstrated with the aid of experimental results.

  3. Minimizing camera-eye optical aberrations during the 3D reconstruction of retinal structures

    NASA Astrophysics Data System (ADS)

    Aldana-Iuit, Javier; Martinez-Perez, M. Elena; Espinosa-Romero, Arturo; Diaz-Uribe, Rufino

    2010-05-01

    3D reconstruction of blood vessels is a powerful visualization tool for physicians, since it allows them to refer to qualitative representation of their subject of study. In this paper we propose a 3D reconstruction method of retinal vessels from fundus images. The reconstruction method propose herein uses images of the same retinal structure in epipolar geometry. Images are preprocessed by RISA system for segmenting blood vessels and obtaining feature points for correspondences. The correspondence points process is solved using correlation. The LMedS analysis and Graph Transformation Matching algorithm are used for outliers suppression. Camera projection matrices are computed with the normalized eight point algorithm. Finally, we retrieve 3D position of the retinal tree points by linear triangulation. In order to increase the power of visualization, 3D tree skeletons are represented by surfaces via generalized cylinders whose radius correspond to morphological measurements obtained by RISA. In this paper the complete calibration process including the fundus camera and the optical properties of the eye, the so called camera-eye system is proposed. On one hand, the internal parameters of the fundus camera are obtained by classical algorithms using a reference pattern. On the other hand, we minimize the undesirable efects of the aberrations induced by the eyeball optical system assuming that contact enlarging lens corrects astigmatism, spherical and coma aberrations are reduced changing the aperture size and eye refractive errors are suppressed adjusting camera focus during image acquisition. Evaluation of two self-calibration proposals and results of 3D blood vessel surface reconstruction are presented.

  4. Design of 3D isotropic metamaterial device using smart transformation optics.

    PubMed

    Shin, Dongheok; Kim, Junhyun; Yoo, Do-Sik; Kim, Kyoungsik

    2015-08-24

    We report here a design method for a 3 dimensional (3D) isotropic transformation optical device using smart transformation optics. Inspired by solid mechanics, smart transformation optics regards a transformation optical medium as an elastic solid and deformations as coordinate transformations. Further developing from our previous work on 2D smart transformation optics, we introduce a method of 3D smart transformation optics to design 3D transformation optical devices by maintaining isotropic materials properties for all types of polarizations imposing free or nearly free boundary conditions. Due to the material isotropy, it is possible to fabricate such devices with structural metamaterials made purely of common dielectric materials. In conclusion, the practical importance of the method reported here lies in the fact that it enables us to fabricate, without difficulty, arbitrarily shaped 3D devices with existing 3D printing technology.

  5. 3D Printing of Molecular Potential Energy Surface Models

    ERIC Educational Resources Information Center

    Lolur, Phalgun; Dawes, Richard

    2014-01-01

    Additive manufacturing, commonly known as 3D printing, is gaining popularity in a variety of applications and has recently become routinely available. Today, 3D printing services are not only found in engineering design labs and through online companies, but also in university libraries offering student access. In addition, affordable options for…

  6. A new way to characterize autostereoscopic 3D displays using Fourier optics instrument

    NASA Astrophysics Data System (ADS)

    Boher, P.; Leroux, T.; Bignon, T.; Collomb-Patton, V.

    2009-02-01

    Auto-stereoscopic 3D displays offer presently the most attractive solution for entertainment and media consumption. Despite many studies devoted to this type of technology, efficient characterization methods are still missing. We present here an innovative optical method based on high angular resolution viewing angle measurements with Fourier optics instrument. This type of instrument allows measuring the full viewing angle aperture of the display very rapidly and accurately. The system used in the study presents a very high angular resolution below 0.04 degree which is mandatory for this type of characterization. We can predict from the luminance or color viewing angle measurements of the different views of the 3D display what will be seen by an observer at any position in front of the display. Quality criteria are derived both for 3D and standard properties at any observer position and Qualified Stereo Viewing Space (QSVS) is determined. The use of viewing angle measurements at different locations on the display surface during the observer computation gives more realistic estimation of QSVS and ensures its validity for the entire display surface. Optimum viewing position, viewing freedom, color shifts and standard parameters are also quantified. Simulation of the moire issues can be made leading to a better understanding of their origin.

  7. Full-field 3D shape measurement of specular surfaces by direct phase to depth relationship

    NASA Astrophysics Data System (ADS)

    Zhang, Zonghua; Liu, Yue; Huang, Shujun; Niu, Zhenqi; Guo, Jiao; Gao, Nan; Gao, Feng; Jiang, Xiangqian

    2016-11-01

    This paper presents a new Phase Measuring Deflectometry (PMD) method to measure specular object having discontinuous surfaces. A mathematical model is established to directly relate absolute phase and depth, instead of phase and gradient. Based on the model, a hardware measuring system has been set up, which consists of a beam splitter to change the optical path, and two LCD screens to display the same sinusoidal fringe patterns. By using model-based and machine vision method, system calibration is accomplished to provide the required parameters and conditions. The verification tests are given to evaluate the effectiveness of the developed system. The 3D shape of an artificial step having multiple specular surfaces and a concave mirror has been measured. Initial experimental results show that the proposed measurement method can obtain 3D shape of specular objects with discontinuous surface effectively.

  8. Surface modified alginate microcapsules for 3D cell culture

    NASA Astrophysics Data System (ADS)

    Chen, Yi-Wen; Kuo, Chiung Wen; Chueh, Di-Yen; Chen, Peilin

    2016-06-01

    Culture as three dimensional cell aggregates or spheroids can offer an ideal platform for tissue engineering applications and for pharmaceutical screening. Such 3D culture models, however, may suffer from the problems such as immune response and ineffective and cumbersome culture. This paper describes a simple method for producing microcapsules with alginate cores and a thin shell of poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) to encapsulate mouse induced pluripotent stem (miPS) cells, generating a non-fouling surface as an effective immunoisolation barrier. We demonstrated the trapping of the alginate microcapsules in a microwell array for the continuous observation and culture of a large number of encapsulated miPS cells in parallel. miPS cells cultured in the microcapsules survived well and proliferated to form a single cell aggregate. Droplet formation of monodisperse microcapsules with controlled size combined with flow cytometry provided an efficient way to quantitatively analyze the growth of encapsulated cells in a high-throughput manner. The simple and cost-effective coating technique employed to produce the core-shell microcapsules could be used in the emerging field of cell therapy. The microwell array would provide a convenient, user friendly and high-throughput platform for long-term cell culture and monitoring.

  9. Multiframe image point matching and 3-d surface reconstruction.

    PubMed

    Tsai, R Y

    1983-02-01

    This paper presents two new methods, the Joint Moment Method (JMM) and the Window Variance Method (WVM), for image matching and 3-D object surface reconstruction using multiple perspective views. The viewing positions and orientations for these perspective views are known a priori, as is usually the case for such applications as robotics and industrial vision as well as close range photogrammetry. Like the conventional two-frame correlation method, the JMM and WVM require finding the extrema of 1-D curves, which are proved to theoretically approach a delta function exponentially as the number of frames increases for the JMM and are much sharper than the two-frame correlation function for both the JMM and the WVM, even when the image point to be matched cannot be easily distinguished from some of the other points. The theoretical findings have been supported by simulations. It is also proved that JMM and WVM are not sensitive to certain radiometric effects. If the same window size is used, the computational complexity for the proposed methods is about n - 1 times that for the two-frame method where n is the number of frames. Simulation results show that the JMM and WVM require smaller windows than the two-frame correlation method with better accuracy, and therefore may even be more computationally feasible than the latter since the computational complexity increases quadratically as a function of the window size.

  10. Computational-optical microscopy for 3D biological imaging beyond the diffraction limit

    NASA Astrophysics Data System (ADS)

    Grover, Ginni

    In recent years, super-resolution imaging has become an important fluorescent microscopy tool. It has enabled imaging of structures smaller than the optical diffraction limit with resolution less than 50 nm. Extension to high-resolution volume imaging has been achieved by integration with various optical techniques. In this thesis, development of a fluorescent microscope to enable high resolution, extended depth, three dimensional (3D) imaging is discussed; which is achieved by integration of computational methods with optical systems. In the first part of the thesis, point spread function (PSF) engineering for volume imaging is discussed. A class of PSFs, referred to as double-helix (DH) PSFs, is generated. The PSFs exhibit two focused spots in the image plane which rotate about the optical axis, encoding depth in rotation of the image. These PSFs extend the depth-of-field up to a factor of ˜5. Precision performance of the DH-PSFs, based on an information theoretical analysis, is compared with other 3D methods with conclusion that the DH-PSFs provide the best precision and the longest depth-of-field. Out of various possible DH-PSFs, a suitable PSF is obtained for super-resolution microscopy. The DH-PSFs are implemented in imaging systems, such as a microscope, with a special phase modulation at the pupil plane. Surface-relief elements which are polarization-insensitive and ˜90% light efficient are developed for phase modulation. The photon-efficient DH-PSF microscopes thus developed are used, along with optimal position estimation algorithms, for tracking and super-resolution imaging in 3D. Imaging at depths-of-field of up to 2.5 microm is achieved without focus scanning. Microtubules were imaged with 3D resolution of (6, 9, 39) nm, which is in close agreement with the theoretical limit. A quantitative study of co-localization of two proteins in volume was conducted in live bacteria. In the last part of the thesis practical aspects of the DH-PSF microscope are

  11. 3D optical Yagi–Uda nanoantenna array

    PubMed Central

    Dregely, Daniel; Taubert, Richard; Dorfmüller, Jens; Vogelgesang, Ralf; Kern, Klaus; Giessen, Harald

    2011-01-01

    Future photonic circuits with the capability of high-speed data processing at optical frequencies will rely on the implementation of efficient emitters and detectors on the nanoscale. Towards this goal, bridging the size mismatch between optical radiation and subwavelength emitters or detectors by optical nanoantennas is a subject of current research in the field of plasmonics. Here we introduce an array of three-dimensional optical Yagi–Uda antennas, fabricated using top-down fabrication techniques combined with layer-by-layer processing. We show that the concepts of radiofrequency antenna arrays can be applied to the optical regime proving superior directional properties compared with a single planar optical antenna, particularly for emission and reception into the third dimension. Measuring the optical properties of the structure reveals that impinging light on the array is efficiently absorbed on the subwavelength scale because of the high directivity. Moreover, we show in simulations that combining the array with suitable feeding circuits gives rise to the prospect of beam steering at optical wavelengths. PMID:21468019

  12. 3D optical printing of piezoelectric nanoparticle-polymer composite materials.

    PubMed

    Kim, Kanguk; Zhu, Wei; Qu, Xin; Aaronson, Chase; McCall, William R; Chen, Shaochen; Sirbuly, Donald J

    2014-10-28

    Here we demonstrate that efficient piezoelectric nanoparticle-polymer composite materials can be optically printed into three-dimensional (3D) microstructures using digital projection printing. Piezoelectric polymers were fabricated by incorporating barium titanate (BaTiO3, BTO) nanoparticles into photoliable polymer solutions such as polyethylene glycol diacrylate and exposing to digital optical masks that could be dynamically altered to generate user-defined 3D microstructures. To enhance the mechanical-to-electrical conversion efficiency of the composites, the BTO nanoparticles were chemically modified with acrylate surface groups, which formed direct covalent linkages with the polymer matrix under light exposure. The composites with a 10% mass loading of the chemically modified BTO nanoparticles showed piezoelectric coefficients (d(33)) of ∼ 40 pC/N, which were over 10 times larger than composites synthesized with unmodified BTO nanoparticles and over 2 times larger than composites containing unmodified BTO nanoparticles and carbon nanotubes to boost mechanical stress transfer efficiencies. These results not only provide a tool for fabricating 3D piezoelectric polymers but lay the groundwork for creating highly efficient piezoelectric polymer materials via nanointerfacial tuning.

  13. Parallel Imaging of 3D Surface Profile with Space-Division Multiplexing

    PubMed Central

    Lee, Hyung Seok; Cho, Soon-Woo; Kim, Gyeong Hun; Jeong, Myung Yung; Won, Young Jae; Kim, Chang-Seok

    2016-01-01

    We have developed a modified optical frequency domain imaging (OFDI) system that performs parallel imaging of three-dimensional (3D) surface profiles by using the space division multiplexing (SDM) method with dual-area swept sourced beams. We have also demonstrated that 3D surface information for two different areas could be well obtained in a same time with only one camera by our method. In this study, double field of views (FOVs) of 11.16 mm × 5.92 mm were achieved within 0.5 s. Height range for each FOV was 460 µm and axial and transverse resolutions were 3.6 and 5.52 µm, respectively. PMID:26805840

  14. Polysilane-based 3D waveguides for optical interconnects

    NASA Astrophysics Data System (ADS)

    Ogura, Kouhei; Oka, Takeshi; Watanabe, Emi; Aoi, Kazunori; Tsushima, Hiroshi; Okano, Hiroaki; Suzuki, Shuji; Hiramatsu, Seiki

    2008-02-01

    We have been developing the optical waveguide for the multimode using the photo-bleaching property of polysilane. The refractive index of polysilane can be easily changed by exposing to UV light as photobleaching. Using this property, we can make waveguide with simple processes as spin coating, exposing and annealing. We found that this waveguide has thermal adhesive property with glass substrate. And we applied this feature to fabricate multilayered optical waveguides that have three-dimensional structure and can change the optical light at right angle.

  15. Focusing optics of a parallel beam CCD optical tomography apparatus for 3D radiation gel dosimetry.

    PubMed

    Krstajić, Nikola; Doran, Simon J

    2006-04-21

    Optical tomography of gel dosimeters is a promising and cost-effective avenue for quality control of radiotherapy treatments such as intensity-modulated radiotherapy (IMRT). Systems based on a laser coupled to a photodiode have so far shown the best results within the context of optical scanning of radiosensitive gels, but are very slow ( approximately 9 min per slice) and poorly suited to measurements that require many slices. Here, we describe a fast, three-dimensional (3D) optical computed tomography (optical-CT) apparatus, based on a broad, collimated beam, obtained from a high power LED and detected by a charged coupled detector (CCD). The main advantages of such a system are (i) an acquisition speed approximately two orders of magnitude higher than a laser-based system when 3D data are required, and (ii) a greater simplicity of design. This paper advances our previous work by introducing a new design of focusing optics, which take information from a suitably positioned focal plane and project an image onto the CCD. An analysis of the ray optics is presented, which explains the roles of telecentricity, focusing, acceptance angle and depth-of-field (DOF) in the formation of projections. A discussion of the approximation involved in measuring the line integrals required for filtered backprojection reconstruction is given. Experimental results demonstrate (i) the effect on projections of changing the position of the focal plane of the apparatus, (ii) how to measure the acceptance angle of the optics, and (iii) the ability of the new scanner to image both absorbing and scattering gel phantoms. The quality of reconstructed images is very promising and suggests that the new apparatus may be useful in a clinical setting for fast and accurate 3D dosimetry.

  16. Kinect the dots: 3D control of optical tweezers

    NASA Astrophysics Data System (ADS)

    Shaw, Lucy; Preece, Daryl; Rubinsztein-Dunlop, Halina

    2013-07-01

    Holographically generated optical traps confine micron- and sub-micron sized particles close to the center of focused light beams. They also provide a way of trapping multiple particles and moving them in three dimensions. However, in many systems the user interface is not always advantageous or intuitive especially for collaborative work and when depth information is required. We discuss and evaluate a set of multi-beam optical tweezers that utilize off the shelf gaming technology to facilitate user interaction. We use the Microsoft Kinect sensor bar as a way of getting the user input required to generate arbitrary optical force fields and control optically trapped particles. We demonstrate that the system can also be used for dynamic light control.

  17. An Optically-Assisted 3-D Cellular Array Machine

    DTIC Science & Technology

    1993-11-05

    Presented by: Physical Optics Corporation 0 Research & Development Division 20600 Gramercy Place, Suite 103 Torrance, California 90501 Principal...Computer Machine (Constructed Hardware) (Planned Hardware Design) Processing Techniques Digital Only Digital and Analog Analog Processor N/A Celular Neural

  18. Brightness-compensated 3-D optical flow algorithm for monitoring cochlear motion patterns

    NASA Astrophysics Data System (ADS)

    von Tiedemann, Miriam; Fridberger, Anders; Ulfendahl, Mats; de Monvel, Jacques Boutet

    2010-09-01

    A method for three-dimensional motion analysis designed for live cell imaging by fluorescence confocal microscopy is described. The approach is based on optical flow computation and takes into account brightness variations in the image scene that are not due to motion, such as photobleaching or fluorescence variations that may reflect changes in cellular physiology. The 3-D optical flow algorithm allowed almost perfect motion estimation on noise-free artificial sequences, and performed with a relative error of <10% on noisy images typical of real experiments. The method was applied to a series of 3-D confocal image stacks from an in vitro preparation of the guinea pig cochlea. The complex motions caused by slow pressure changes in the cochlear compartments were quantified. At the surface of the hearing organ, the largest motion component was the transverse one (normal to the surface), but significant radial and longitudinal displacements were also present. The outer hair cell displayed larger radial motion at their basolateral membrane than at their apical surface. These movements reflect mechanical interactions between different cellular structures, which may be important for communicating sound-evoked vibrations to the sensory cells. A better understanding of these interactions is important for testing realistic models of cochlear mechanics.

  19. Brightness-compensated 3-D optical flow algorithm for monitoring cochlear motion patterns.

    PubMed

    von Tiedemann, Miriam; Fridberger, Anders; Ulfendahl, Mats; de Monvel, Jacques Boutet

    2010-01-01

    A method for three-dimensional motion analysis designed for live cell imaging by fluorescence confocal microscopy is described. The approach is based on optical flow computation and takes into account brightness variations in the image scene that are not due to motion, such as photobleaching or fluorescence variations that may reflect changes in cellular physiology. The 3-D optical flow algorithm allowed almost perfect motion estimation on noise-free artificial sequences, and performed with a relative error of <10% on noisy images typical of real experiments. The method was applied to a series of 3-D confocal image stacks from an in vitro preparation of the guinea pig cochlea. The complex motions caused by slow pressure changes in the cochlear compartments were quantified. At the surface of the hearing organ, the largest motion component was the transverse one (normal to the surface), but significant radial and longitudinal displacements were also present. The outer hair cell displayed larger radial motion at their basolateral membrane than at their apical surface. These movements reflect mechanical interactions between different cellular structures, which may be important for communicating sound-evoked vibrations to the sensory cells. A better understanding of these interactions is important for testing realistic models of cochlear mechanics.

  20. X-Ray and Optical Videography for 3D Measurement of Capillary and Melt Pool Geometry in Laser Welding

    NASA Astrophysics Data System (ADS)

    Boley, M.; Abt, F.; Weber, R.; Graf, T.

    This paper describes a method to reconstruct the 3D shape of the melt pool and the capillary of a laser keyhole welding process. Three different diagnostic methods, including X-Ray and optical videography as well as metallographic cross sections are combined to gain the three dimensional data of the solidus-liquidus-surface. A detailed description of the experimental setup and a discussion of different methods to combine the 2D data sets of the three different diagnostic methods to a 3D-model will be given. The result will be a static 3D description of the welding process.

  1. 3D reconstruction of light flux distribution on arbitrary surfaces from 2D multi-photographic images.

    PubMed

    Chen, Xueli; Gao, Xinbo; Chen, Duofang; Ma, Xiaopeng; Zhao, Xiaohui; Shen, Man; Li, Xiangsi; Qu, Xiaochao; Liang, Jimin; Ripoll, Jorge; Tian, Jie

    2010-09-13

    Optical tomography can demonstrate accurate three-dimensional (3D) imaging that recovers the 3D spatial distribution and concentration of the luminescent probes in biological tissues, compared with planar imaging. However, the tomographic approach is extremely difficult to implement due to the complexity in the reconstruction of 3D surface flux distribution from multi-view two dimensional (2D) measurements on the subject surface. To handle this problem, a novel and effective method is proposed in this paper to determine the surface flux distribution from multi-view 2D photographic images acquired by a set of non-contact detectors. The method is validated with comparison experiments involving both regular and irregular surfaces. Reconstruction of the inside probes based on the reconstructed surface flux distribution further demonstrates the potential of the proposed method in its application in optical tomography.

  2. Parallel beam optical tomography apparatus for 3D radiation dosimetry

    NASA Astrophysics Data System (ADS)

    Krstajic, Nikola; Doran, Simon J.

    2005-06-01

    Since the discovery of X rays radiotherapy has had the same aim - to deliver a precisely measured dose of radiation to a defined tumour volume with minimal damage to surrounding healthy tissue. Recent developments in radiotherapy such as intensity modulated radiotherapy (IMRT) can generate complex shapes of dose distributions. Until recently it has not been possible to verify that the delivered dose matches the planned dose. However, one often wants to know the real three-dimensional dose distribution. Three-dimensional radiation dosimeters have been developed since the early 1980s. Most chemical formulations involve a radiosensitive species immobilised in space by gelling agent. Magnetic Resonance Imaging (MRI) and optical techniques have been the most successful gel scanning techniques so far. Optical techniques rely on gels changing colour once irradiated. Parallel beam optical tomography has been developed at the University of Surrey since the late 1990s. The apparatus involves light emitting diode light source collimated to a wide (12cm) parallel beam. The beam is attenuated or scattered (depending on the chemical formulation) as it passes through the gel. Focusing optics projects the beam onto a CCD chip. The dosimeter sits on a rotation stage. The tomography scan involves continuously rotating the dosimeter and taking CCD images. Once the dosimeter has been rotated over 180 degrees the images are processed by filtered back projection. The work presented discusses the optics of the apparatus in more detail.

  3. 3D transient model to predict temperature and ablated areas during laser processing of metallic surfaces

    NASA Astrophysics Data System (ADS)

    Naghshine, Babak. B.; Kiani, Amirkianoosh

    2017-02-01

    Laser processing is one of the most popular small-scale patterning methods and has many applications in semiconductor device fabrication and biomedical engineering. Numerical modelling of this process can be used for better understanding of the process, optimization, and predicting the quality of the final product. An accurate 3D model is presented here for short laser pulses that can predict the ablation depth and temperature distribution on any section of the material in a minimal amount of time. In this transient model, variations of thermal properties, plasma shielding, and phase change are considered. Ablation depth was measured using a 3D optical profiler. Calculated depths are in good agreement with measured values on laser treated titanium surfaces. The proposed model can be applied to a wide range of materials and laser systems.

  4. State of the art of 3D scanning systems and inspection of textile surfaces

    NASA Astrophysics Data System (ADS)

    Montilla, M.; Orjuela-Vargas, S. A.; Philips, W.

    2014-02-01

    The rapid development of hardware and software in the digital image processing field has boosted research in computer vision for applications in industry. The development of new electronic devices and the tendency to decrease their prices makes possible new developments that few decades ago were possible only in the imagination. This is the case of 3D imaging technology which permits to detect failures in industrial products by inspecting aspects on their 3D surface. In search of an optimal solution for scanning textiles we present in this paper a review of existing techniques for digitizing 3D surfaces. Topographic details of textiles can be obtained by digitizing surfaces using laser line triangulation, phase shifting optical triangulation, projected-light, stereo-vision systems and silhouette analysis. Although we are focused on methods that have been used in the textile industry, we also consider potential mechanisms used for other applications. We discuss the advantages and disadvantages of the evaluated methods and state a summary of potential implementations for the textile industry.

  5. Towards a 3-D Magneto-Optical Trap for Molecules

    NASA Astrophysics Data System (ADS)

    Collopy, Alejandra; Hummon, Matthew; Yeo, Mark; Stuhl, Benjamin; Hemmerling, Boerge; Drayna, Garrett; Chae, Eunmi; Ravi, Aakash; Lu, Hsin-I.; Doyle, John; Ye, Jun

    2013-05-01

    As the magneto-optical trap revolutionized atomic physics, we anticipate the molecular counterpart to open doors to unexplored molecular physics, including ultra-cold chemistry. While molecules possess more complex structure than atoms, quasi-cycling cooling transitions are still attainable in a variety of species, including the polar molecule YO. In order to remix dark states, we RF modulate the polarization of the light in our trap. In order to maintain a restoring force, we modulate the orientation of our magnetic fields in phase with the light using LC resonant in-vacuum magnetic coils. We demonstrate magneto-optical trapping in two dimensions for YO, and present progress towards a three dimensional implementation of a MOT loaded from a two-stage buffer gas cell source. We acknowledge support from the AFOSR (MURI), DOE, NIST and the NSF.

  6. Rotating and Precessing Dissipative-Optical-Topological-3D Solitons

    NASA Astrophysics Data System (ADS)

    Veretenov, N. A.; Rosanov, N. N.; Fedorov, S. V.

    2016-10-01

    We predict and study a new type of three-dimensional soliton: asymmetric rotating and precessing stable topological-dissipative-optical localized structures in homogeneous media with saturable amplification and absorption. The crucial factor determining their dynamics is the ratio of the diffusion coefficients characterizing the frequency dispersion and angular selectivity (dichroism) of the scheme. These vortex solitons exist and are stable for overcritical values of the selectivity coefficients and can be realized in lasers of large sizes with saturable absorption.

  7. Cytology 3D structure formation based on optical microscopy images

    NASA Astrophysics Data System (ADS)

    Pronichev, A. N.; Polyakov, E. V.; Shabalova, I. P.; Djangirova, T. V.; Zaitsev, S. M.

    2017-01-01

    The article the article is devoted to optimization of the parameters of imaging of biological preparations in optical microscopy using a multispectral camera in visible range of electromagnetic radiation. A model for the image forming of virtual preparations was proposed. The optimum number of layers was determined for the object scan in depth and holistic perception of its switching according to the results of the experiment.

  8. A 3D glass optrode array for optical neural stimulation

    PubMed Central

    Abaya, T.V.F.; Blair, S.; Tathireddy, P.; Rieth, L.; Solzbacher, F.

    2012-01-01

    This paper presents optical characterization of a first-generation SiO2 optrode array as a set of penetrating waveguides for both optogenetic and infrared (IR) neural stimulation. Fused silica and quartz discs of 3-mm thickness and 50-mm diameter were micromachined to yield 10 × 10 arrays of up to 2-mm long optrodes at a 400-μm pitch; array size, length and spacing may be varied along with the width and tip angle. Light delivery and loss mechanisms through these glass optrodes were characterized. Light in-coupling techniques include using optical fibers and collimated beams. Losses involve Fresnel reflection, coupling, scattering and total internal reflection in the tips. Transmission efficiency was constant in the visible and near-IR range, with the highest value measured as 71% using a 50-μm multi-mode in-coupling fiber butt-coupled to the backplane of the device. Transmittance and output beam profiles of optrodes with different geometries was investigated. Length and tip angle do not affect the amount of output power, but optrode width and tip angle influence the beam size and divergence independently. Finally, array insertion in tissue was performed to demonstrate its robustness for optical access in deep tissue. PMID:23243561

  9. 3-D Adaptive Sparsity Based Image Compression with Applications to Optical Coherence Tomography

    PubMed Central

    Fang, Leyuan; Li, Shutao; Kang, Xudong; Izatt, Joseph A.; Farsiu, Sina

    2015-01-01

    We present a novel general-purpose compression method for tomographic images, termed 3D adaptive sparse representation based compression (3D-ASRC). In this paper, we focus on applications of 3D-ASRC for the compression of ophthalmic 3D optical coherence tomography (OCT) images. The 3D-ASRC algorithm exploits correlations among adjacent OCT images to improve compression performance, yet is sensitive to preserving their differences. Due to the inherent denoising mechanism of the sparsity based 3D-ASRC, the quality of the compressed images are often better than the raw images they are based on. Experiments on clinical-grade retinal OCT images demonstrate the superiority of the proposed 3D-ASRC over other well-known compression methods. PMID:25561591

  10. Rapid Prototyping across the Spectrum: RF to Optical 3D Electromagnetic Structures

    DTIC Science & Technology

    2015-11-17

    fabricated using 3D printer . The fill factor decreases radially outwards and the voids are visible in the unit cells as you approach the periphery of the...with thin walls) [29]. Figure 6: Examples of lenses fabricated with AM (a) GRIN lens fabricated using 3D printer . The fill factor decreases...AFRL-RW-EG-TP-2015-002 Rapid Prototyping across the Spectrum: RF to Optical 3D Electromagnetic Structures Jeffery W. Allen Monica S. Allen Brett

  11. 3D-confocal microscopy for surface analysis of microstructured materials

    NASA Astrophysics Data System (ADS)

    Kagerer, Bernd; Brodmann, Rainer; Valentin, Juergen; Filzek, Jan; Popp, Uwe

    2002-06-01

    The surface of technical materials is playing an ever more important part in modern production processes. However, standard roughness values, which are obtained from a profile, frequently no longer provide sufficient descriptions. What are desired are three-dimensional measurements of surfaces over a macroscopic range with a high degree of vertical and lateral resolution. This has become necessary to be able to describe both deterministic and non-deterministic structures in the same fashion. Due to increased requirements for data and the measuring speed demanded by industry, only optical systems are a possibility. Using the example of tribology, the capability of this technology is shown in this article on the basis of the commercial confocal 3D white light microscope, the NanoFocusTMμSurfTM. On the one hand, the technology and data preparation used are discussed, and on the other, a comparison is drawn with other standard optical measuring methods.

  12. Underwater 3D Surface Measurement Using Fringe Projection Based Scanning Devices

    PubMed Central

    Bräuer-Burchardt, Christian; Heinze, Matthias; Schmidt, Ingo; Kühmstedt, Peter; Notni, Gunther

    2015-01-01

    In this work we show the principle of optical 3D surface measurements based on the fringe projection technique for underwater applications. The challenges of underwater use of this technique are shown and discussed in comparison with the classical application. We describe an extended camera model which takes refraction effects into account as well as a proposal of an effective, low-effort calibration procedure for underwater optical stereo scanners. This calibration technique combines a classical air calibration based on the pinhole model with ray-based modeling and requires only a few underwater recordings of an object of known length and a planar surface. We demonstrate a new underwater 3D scanning device based on the fringe projection technique. It has a weight of about 10 kg and the maximal water depth for application of the scanner is 40 m. It covers an underwater measurement volume of 250 mm × 200 mm × 120 mm. The surface of the measurement objects is captured with a lateral resolution of 150 μm in a third of a second. Calibration evaluation results are presented and examples of first underwater measurements are given. PMID:26703624

  13. 3D Printing Optical Engine for Controlling Material Microstructure

    NASA Astrophysics Data System (ADS)

    Huang, Wei-Chin; Chang, Kuang-Po; Wu, Ping-Han; Wu, Chih-Hsien; Lin, Ching-Chih; Chuang, Chuan-Sheng; Lin, De-Yau; Liu, Sung-Ho; Horng, Ji-Bin; Tsau, Fang-Hei

    Controlling the cooling rate of alloy during melting and resolidification is the most commonly used method for varying the material microstructure and consequently the resuling property. However, the cooling rate of a selective laser melting (SLM) production is restricted by a preset optimal parameter of a good dense product. The head room for locally manipulating material property in a process is marginal. In this study, we invent an Optical Engine for locally controlling material microstructure in a SLM process. It develops an invovative method to control and adjust thermal history of the solidification process to gain desired material microstucture and consequently drastically improving the quality. Process parameters selected locally for specific materials requirement according to designed characteristics by using thermal dynamic principles of solidification process. It utilize a technique of complex laser beam shape of adaptive irradiation profile to permit local control of material characteristics as desired. This technology could be useful for industrial application of medical implant, aerospace and automobile industries.

  14. Full optical characterization of autostereoscopic 3D displays using local viewing angle and imaging measurements

    NASA Astrophysics Data System (ADS)

    Boher, Pierre; Leroux, Thierry; Bignon, Thibault; Collomb-Patton, Véronique

    2012-03-01

    Two commercial auto-stereoscopic 3D displays are characterized a using Fourier optics viewing angle system and an imaging video-luminance-meter. One display has a fixed emissive configuration and the other adapts its emission to the observer position using head tracking. For a fixed emissive condition, three viewing angle measurements are performed at three positions (center, right and left). Qualified monocular and binocular viewing spaces in front of the display are deduced as well as the best working distance. The imaging system is then positioned at this working distance and crosstalk homogeneity on the entire surface of the display is measured. We show that the crosstalk is generally not optimized on all the surface of the display. Display aspect simulation using viewing angle measurements allows understanding better the origin of those crosstalk variations. Local imperfections like scratches and marks generally increase drastically the crosstalk, demonstrating that cleanliness requirements for this type of display are quite critical.

  15. Surface strain-field determination of tympanic membrane using 3D-digital holographic interferometry

    NASA Astrophysics Data System (ADS)

    Hernandez-Montes, María del S.; Mendoza Santoyo, Fernando; Muñoz, Silvino; Perez, Carlos; de la Torre, Manuel; Flores, Mauricio; Alvarez, Luis

    2015-08-01

    In order to increase the understanding of soft tissues mechanical properties, 3D Digital Holographic Interferometry (3D-DHI) was used to quantify the strain-field on a cat tympanic membrane (TM) surface. The experiments were carried out applying a constant sound-stimuli pressure of 90 dB SPL (0.632 Pa) on the TM at 1.2 kHz. The technique allows the accurate acquisition of the micro-displacement data along the x, y and z directions, which is a must for a full characterization of the tissue mechanical behavior under load, and for the calculation of the strain-field in situ. The displacements repeatability in z direction shows a standard deviation of 0.062 μm at 95% confidence level. In order to realize the full 3D characterization correctly the contour of the TM surface was measured employing the optically non-contact two-illumination positions contouring method. The x, y and z displacements combined with the TM contour data allow the evaluation its strain-field by spatially differentiating the u(m,n), v(m,n), and w(m,n) deformation components. The accurate and correct determination of the TM strain-field leads to describing its elasticity, which is an important parameter needed to improve ear biomechanics studies, audition processes and TM mobility in both experimental measurements and theoretical analysis of ear functionality and its modeling.

  16. Optical rotation compensation for a holographic 3D display with a 360 degree horizontal viewing zone.

    PubMed

    Sando, Yusuke; Barada, Daisuke; Yatagai, Toyohiko

    2016-10-20

    A method for a continuous optical rotation compensation in a time-division-based holographic three-dimensional (3D) display with a rotating mirror is presented. Since the coordinate system of wavefronts after the mirror reflection rotates about the optical axis along with the rotation angle, compensation or cancellation is absolutely necessary to fix the reconstructed 3D object. In this study, we address this problem by introducing an optical image rotator based on a right-angle prism that rotates synchronously with the rotating mirror. The optical and continuous compensation reduces the occurrence of duplicate images, which leads to the improvement of the quality of reconstructed images. The effect of the optical rotation compensation is experimentally verified and a demonstration of holographic 3D display with the optical rotation compensation is presented.

  17. An optical real-time 3D measurement for analysis of facial shape and movement

    NASA Astrophysics Data System (ADS)

    Zhang, Qican; Su, Xianyu; Chen, Wenjing; Cao, Yiping; Xiang, Liqun

    2003-12-01

    Optical non-contact 3-D shape measurement provides a novel and useful tool for analysis of facial shape and movement in presurgical and postsurgical regular check. In this article we present a system, which allows a precise 3-D visualization of the patient's facial before and after craniofacial surgery. We discussed, in this paper, the real time 3-D image capture, processing and the 3-D phase unwrapping method to recover complex shape deformation when the movement of the mouth. The result of real-time measurement for facial shape and movement will be helpful for the more ideal effect in plastic surgery.

  18. Searching surface orientation of microscopic objects for accurate 3D shape recovery.

    PubMed

    Shim, Seong-O; Mahmood, Muhammad Tariq; Choi, Tae-Sun

    2012-05-01

    In this article, we propose a new shape from focus (SFF) method to estimate 3D shape of microscopic objects using surface orientation cue of each object patch. Most of the SFF algorithms compute the focus value of a pixel from the information of neighboring pixels lying on the same image frame based on an assumption that the small object patch corresponding to the small neighborhood of a pixel is a plane parallel to the focal plane. However, this assumption fails in the optics with limited depth of field where the neighboring pixels of an image have different degree of focus. To overcome this problem, we try to search the surface orientation of the small object patch corresponding to each pixel in the image sequence. Searching of the surface orientation is done indirectly by principal component analysis. Then, the focus value of each pixel is computed from the neighboring pixels lying on the surface perpendicular to the corresponding surface orientation. Experimental results on synthetic and real microscopic objects show that the proposed method produces more accurate 3D shape in comparison to the existing techniques.

  19. 3D objects enlargement technique using an optical system and multiple SLMs for electronic holography.

    PubMed

    Yamamoto, Kenji; Ichihashi, Yasuyuki; Senoh, Takanori; Oi, Ryutaro; Kurita, Taiichiro

    2012-09-10

    One problem in electronic holography, which is caused by the display performance of spatial light modulators (SLM), is that the size of reconstructed 3D objects is small. Although methods for increasing the size using multiple SLMs have been considered, they typically had the problem that some parts of 3D objects were missing as a result of the gap between adjacent SLMs or 3D objects lost the vertical parallax. This paper proposes a method of resolving this problem by locating an optical system containing a lens array and other components in front of multiple SLMs. We used an optical system and 9 SLMs to construct a device equivalent to an SLM with approximately 74,600,000 pixels and used this to reconstruct 3D objects in both the horizontal and vertical parallax with an image size of 63 mm without losing any part of 3D objects.

  20. Laminar optical tomography: high-resolution 3D functional imaging of superficial tissues

    NASA Astrophysics Data System (ADS)

    Hillman, Elizabeth M. C.; Devor, Anna; Dunn, Andrew K.; Boas, David A.

    2006-03-01

    Laminar Optical Tomography (LOT) is a new medical imaging modality for high-resolution, depth-resolved, functional imaging of superficial tissue such as rodent cortex, skin and the retina. LOT uses visible laser light to image to depths of >2mm (far deeper than microscopy) and is highly sensitive to absorption and fluorescence contrast, enabling spectroscopic functional information such as hemoglobin oxygenation to be imaged with 100-200 micron resolution. LOT has been used to image the hemodynamic response to stimulus in the somatosensory cortex of rats. The resulting three-dimensional (3D) images through the depth of the cortex can be used to delineate the arterial, capillary and venous responses, revealing new information about the intricacies of the oxygenation and blood flow dynamics related to neuronal activation. Additional applications of LOT are being explored, including the integration of 3D Voltage Sensitive Dye fluorescence imaging. LOT imaging uses a system similar to a confocal microscope, quickly scanning a focused beam of light over the surface of the tissue (~8Hz frame rate). Light is detected from both the focus of the scanning beam, and also at increasing distances from the beam's focus. This scattered light has penetrated more deeply into the tissue, and allows features at different depths to be distinguished. An algorithm that includes photon migration modeling of light scattering converts the raw data into 3D images. The motivation for functional optical imaging will be outlined, the basic principles of LOT imaging will be described, and the latest in-vivo results will be presented.

  1. Evolution of 3D surface imaging systems in facial plastic surgery.

    PubMed

    Tzou, Chieh-Han John; Frey, Manfred

    2011-11-01

    Recent advancements in computer technologies have propelled the development of 3D imaging systems. 3D surface-imaging is taking surgeons to a new level of communication with patients; moreover, it provides quick and standardized image documentation. This article recounts the chronologic evolution of 3D surface imaging, and summarizes the current status of today's facial surface capturing technology. This article also discusses current 3D surface imaging hardware and software, and their different techniques, technologies, and scientific validation, which provides surgeons with the background information necessary for evaluating the systems and knowledge about the systems they might incorporate into their own practice.

  2. Disparity pattern-based autostereoscopic 3D metrology system for in situ measurement of microstructured surfaces.

    PubMed

    Li, Da; Cheung, Chi Fai; Ren, MingJun; Whitehouse, David; Zhao, Xing

    2015-11-15

    This paper presents a disparity pattern-based autostereoscopic (DPA) 3D metrology system that makes use of a microlens array to capture raw 3D information of the measured surface in a single snapshot through a CCD camera. Hence, a 3D digital model of the target surface with the measuring data is generated through a system-associated direct extraction of disparity information (DEDI) method. The DEDI method is highly efficient for performing the direct 3D mapping of the target surface based on tomography-like operation upon every depth plane with the defocused information excluded. Precise measurement results are provided through an error-elimination process based on statistical analysis. Experimental results show that the proposed DPA 3D metrology system is capable of measuring 3D microstructured surfaces with submicrometer measuring repeatability for high precision and in situ measurement of microstructured surfaces.

  3. 3-D micro surface profilometry employing novel Mirau-based lateral scanning interferometry

    NASA Astrophysics Data System (ADS)

    Chen, Liang-Chia; Le, Manh-Trung; Lin, Yi-Shiuan

    2014-09-01

    An innovative 3-D surface imaging methodology for reconstructing micro surface profiles with a long depth measuring range and a nano-scale resolution was developed using the newly developed Mirau-based lateral scanning interferometry (LSI). The current measuring field of view (FOV) of conventional white light interferometers is limited by microscopic views of the existing interferometric objectives, such as those in Michelson, Mirau or Linnik designs. Moreover, the vertical scanning operation required for acquiring volumetric interferometric data is extremely time-consuming and makes white light vertical scanning interferometry (VSI) infeasible for automatic optical inspection (AOI) of micro 3-D structures. To resolve this, a newly developed white light LSI method based on Mirau’s optical configuration was developed by controlling the tilting angle of the reference mirror in the Mirau interferometric objective. With the proposed optical configuration, the surface is inspected at a tilting angle with respect to the maximum coherence plane of the interferometric system along its lateral scanning direction when the objective lies perpendicular to the tested surface. In addition, a system calibration method was developed to establish an accurate mathematical mapping model between the object depth and the lateral axis. To evaluate the feasibility of the methodology, a calibrated step height was measured for evaluating the accuracy and repeatability. Some industrial samples, such as photon spacers and other microstructures fabricated by nano-imprinting processes, were measured to verify the actual performance on real components. It was found that the measurement repeatability was controlled less than 60 nm within one standard deviation for a maximum measurable depth of 27.21 µm.

  4. Pluto: Modeling of 3-D Atmosphere-Surface Interactions

    NASA Astrophysics Data System (ADS)

    Michaels, Timothy I.

    2015-11-01

    Atmosphere-surface interactions on Pluto are of great importance to creating and maintaining the atmospheric variations and heterogeneous surface that have been observed by New Horizons and two decades' prior work. Publicly released images/data from New Horizons contain numerous fascinating surface features and constrasts. Insights into their origin, maintenance, and/or evolution may be gleaned through multidisciplinary climate modeling. Some results from such modeling will be presented, with an emphasis on shorter-timescale interactions.

  5. Integration of 3D anatomical data obtained by CT imaging and 3D optical scanning for computer aided implant surgery

    PubMed Central

    2011-01-01

    Background A precise placement of dental implants is a crucial step to optimize both prosthetic aspects and functional constraints. In this context, the use of virtual guiding systems has been recognized as a fundamental tool to control the ideal implant position. In particular, complex periodontal surgeries can be performed using preoperative planning based on CT data. The critical point of the procedure relies on the lack of accuracy in transferring CT planning information to surgical field through custom-made stereo-lithographic surgical guides. Methods In this work, a novel methodology is proposed for monitoring loss of accuracy in transferring CT dental information into periodontal surgical field. The methodology is based on integrating 3D data of anatomical (impression and cast) and preoperative (radiographic template) models, obtained by both CT and optical scanning processes. Results A clinical case, relative to a fully edentulous jaw patient, has been used as test case to assess the accuracy of the various steps concurring in manufacturing surgical guides. In particular, a surgical guide has been designed to place implants in the bone structure of the patient. The analysis of the results has allowed the clinician to monitor all the errors, which have been occurring step by step manufacturing the physical templates. Conclusions The use of an optical scanner, which has a higher resolution and accuracy than CT scanning, has demonstrated to be a valid support to control the precision of the various physical models adopted and to point out possible error sources. A case study regarding a fully edentulous patient has confirmed the feasibility of the proposed methodology. PMID:21338504

  6. 3D optical simulation formalism OPTOS for textured silicon solar cells.

    PubMed

    Tucher, Nico; Eisenlohr, Johannes; Kiefel, Peter; Höhn, Oliver; Hauser, Hubert; Peters, Marius; Müller, Claas; Goldschmidt, Jan Christoph; Bläsi, Benedikt

    2015-11-30

    In this paper we introduce the three-dimensional formulation of the OPTOS formalism, a matrix-based method that allows for the efficient simulation of non-coherent light propagation and absorption in thick textured sheets. As application examples, we calculate the absorptance of solar cells featuring textures on front and rear side with different feature sizes operating in different optical regimes. A discretization of polar and azimuth angle enables a three-dimensional description of systems with arbitrary surface textures. We present redistribution matrices for 3D surface textures, including pyramidal textures, binary crossed gratings and a Lambertian scatterer. The results of the OPTOS simulations for silicon sheets with different combinations of these surfaces are in accordance with both optical measurements and results based on established simulation methods like ray tracing. Using OPTOS, we show that the integration of a diffractive grating at the rear side of a silicon solar cell featuring a pyramidal front side results in absorption close to the Yablonovitch Limit enhancing the photocurrent density by 0.6 mA/cm2 for a 200 µm thick cell.

  7. 3D imaging of dental hard tissues with Fourier domain optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Chen, Yueli L.; Yang, Yi; Ma, Jing; Yan, Jun; Shou, Yuanxin; Wang, Tianheng; Ramesh, Aruna; Zhao, Jing; Zhu, Quing

    2011-03-01

    A fiber optical coherence tomography (OCT) probe is used for three dimensional dental imaging. The probe has a lightweight miniaturized design with a size of a pen to facilitate clinic in vivo diagnostics. The probe is interfaced with a swept-source / Fourier domain optical coherence tomography at 20K axial scanning rate. The tooth samples were scanned from occlusal, buccal, lingual, mesial, and distal orientations. Three dimensional imaging covers tooth surface area up to 10 mm x 10 mm with a depth about 5 mm, where a majority of caries affection occurs. OCT image provides better resolution and contrast compared to gold standard dental radiography (X-ray). In particular, the technology is well suited for occlusal caries detection. This is complementary to X-ray as occlusal caries affection is difficult to be detected due to the X-ray projectile scan geometry. The 3D topology of occlusal surface as well as the dentin-enamel junction (DEJ) surface inside the tooth can be visualized. The lesion area appears with much stronger back scattering signal intensity.

  8. New algorithms to map asymmetries of 3D surfaces.

    PubMed

    Combès, Benoît; Prima, Sylvain

    2008-01-01

    In this paper, we propose a set of new generic automated processing tools to characterise the local asymmetries of anatomical structures (represented by surfaces) at an individual level, and within/between populations. The building bricks of this toolbox are: (1) a new algorithm for robust, accurate, and fast estimation of the symmetry plane of grossly symmetrical surfaces, and (2) a new algorithm for the fast, dense, nonlinear matching of surfaces. This last algorithm is used both to compute dense individual asymmetry maps on surfaces, and to register these maps to a common template for population studies. We show these two algorithms to be mathematically well-grounded, and provide some validation experiments. Then we propose a pipeline for the statistical evaluation of local asymmetries within and between populations. Finally we present some results on real data.

  9. Automated multilayer segmentation and characterization in 3D spectral-domain optical coherence tomography images

    NASA Astrophysics Data System (ADS)

    Hu, Zhihong; Wu, Xiaodong; Hariri, Amirhossein; Sadda, SriniVas R.

    2013-03-01

    Spectral-domain optical coherence tomography (SD-OCT) is a 3-D imaging technique, allowing direct visualization of retinal morphology and architecture. The various layers of the retina may be affected differentially by various diseases. In this study, an automated graph-based multilayer approach was developed to sequentially segment eleven retinal surfaces including the inner retinal bands to the outer retinal bands in normal SD-OCT volume scans at three different stages. For stage 1, the four most detectable and/or distinct surfaces were identified in the four-times-downsampled images and were used as a priori positional information to limit the graph search for other surfaces at stage 2. Eleven surfaces were then detected in the two-times-downsampled images at stage 2, and refined in the original image space at stage 3 using the graph search integrating the estimated morphological shape models. Twenty macular SD-OCT (Heidelberg Spectralis) volume scans from 20 normal subjects (one eye per subject) were used in this study. The overall mean and absolute mean differences in border positions between the automated and manual segmentation for all 11 segmented surfaces were -0.20 +/- 0.53 voxels (-0.76 +/- 2.06 μm) and 0.82 +/- 0.64 voxels (3.19 +/- 2.46 μm). Intensity and thickness properties in the resultant retinal layers were investigated. This investigation in normal subjects may provide a comparative reference for subsequent investigations in eyes with disease.

  10. Visual Short-Term Memory Benefit for Objects on Different 3-D Surfaces

    ERIC Educational Resources Information Center

    Xu, Yaoda; Nakayama, Ken

    2007-01-01

    Visual short-term memory (VSTM) plays an important role in visual cognition. Although objects are located on different 3-dimensional (3-D) surfaces in the real world, how VSTM capacity may be influenced by the presence of multiple 3-D surfaces has never been examined. By manipulating binocular disparities of visual displays, the authors found that…

  11. Automated Sensor for 3-D Reconstruction of Optical Emission from RF Plasmas

    NASA Astrophysics Data System (ADS)

    Collard, Corey; Shannon, S.; Brake, M. L.; Holloway, James Paul

    1999-10-01

    Three dimensional images are obtained by using an automated scanning sensor which collects optical emission from a RF (13.56 MHz) discharge in a capacitively coupled GEC cell. The sensor scans a plane parallel to the electrode surface and transmits the plasma spectral emission through a fiber optic cable to a monochromator. The fiber optic is attached to a motorized rotational stage attached to a manual vertical translational stage. Wedges of light (argon at 750.4 nm) are collected as the fiber scans across the plasma. The data is digitized and stored so that it can be input into an algorithm, which uses a Tikhonov regularization method to reconstruct the emissivity as a function of radial position. By varying the height of the sensor, a 3-D plot of the plasma emission can be obtained. Three dimensional plots of plasmas run at 75, 100, 150 and 200 peak to peak voltage at pressures of 100, 250, 500 and 1000 mTorr were obtained. The non-uniformity of the light emission as a function of pressure and power will be discussed.

  12. Inspection, 3D modelling, and rapid prototyping of cultural heritage by means of a 3D optical digitiser

    NASA Astrophysics Data System (ADS)

    Docchio, F.; Sansoni, G.; Trebeschi, M.

    2005-06-01

    This paper presents the activity carried out to perform the three-dimensional acquisition of the "Vittoria Alata", a 2m-high, bronze statue, symbol of our City, located at the Civici Musei di Arte e Storia (S. Giulia) of Brescia. The acquisition of the statue has been performed by using a three-dimensional vision system based on active triangulation and on the projection of non-coherent light. This system, called OPL-3D, represents one of the research products of our Laboratory, which has been active for years in the development of techniques and systems for the contactless acquisition of free-form, complex shapes. The study, originally motivated by the need to explore a new hypothesis on the origin of the "Vittoria Alata", led to its complete digitization and description in terms of both polygonal and NURBS-based models. A suite of copies of the whole statue has been obtained in the framework of the collaboration between the City Museum and the EOS Electro Optical Systems GmbH, located in Munich, Germany. As a first step, one 30 cm-high replica of the whole statue has been produced using a low-resolution triangle model of the statue (3.5 millions of triangles). As a second step, two 1:1 scale copies of the statue have been produced. For them, the Laboratory has provided the high resolution STL file (16 millions of triangles). The paper discusses in detail the hardware and the software facilities used to implement the whole process, and gives a comprehensive description of the results.

  13. Cryogenic optical localization provides 3D protein structure data with Angstrom resolution.

    PubMed

    Weisenburger, Siegfried; Boening, Daniel; Schomburg, Benjamin; Giller, Karin; Becker, Stefan; Griesinger, Christian; Sandoghdar, Vahid

    2017-02-01

    We introduce Cryogenic Optical Localization in 3D (COLD), a method to localize multiple fluorescent sites within a single small protein with Angstrom resolution. We demonstrate COLD by determining the conformational state of the cytosolic Per-ARNT-Sim domain from the histidine kinase CitA of Geobacillus thermodenitrificans and resolving the four biotin sites of streptavidin. COLD provides quantitative 3D information about small- to medium-sized biomolecules on the Angstrom scale and complements other techniques in structural biology.

  14. Design of extended viewing zone at autostereoscopic 3D display based on diffusing optical element

    NASA Astrophysics Data System (ADS)

    Kim, Min Chang; Hwang, Yong Seok; Hong, Suk-Pyo; Kim, Eun Soo

    2012-03-01

    In this paper, to realize a non-glasses type 3D display as next step from the current glasses-typed 3D display, it is suggested that a viewing zone is designed for the 3D display using DOE (Diffusing Optical Element). Viewing zone of proposed method is larger than that of the current parallax barrier method or lenticular method. Through proposed method, it is shown to enable the expansion and adjustment of the area of viewing zone according to viewing distance.

  15. Optimized data processing for an optical 3D sensor based on flying triangulation

    NASA Astrophysics Data System (ADS)

    Ettl, Svenja; Arold, Oliver; Häusler, Gerd; Gurov, Igor; Volkov, Mikhail

    2013-05-01

    We present data processing methods for an optical 3D sensor based on the measurement principle "Flying Triangulation". The principle enables a motion-robust acquisition of the 3D shape of even complex objects: A hand-held sensor is freely guided around the object while real-time feedback of the measurement progress is delivered during the captioning. Although of high precision, the resulting 3D data usually may exhibit some weaknesses: e.g. outliers might be present and the data size might be too large. We describe the measurement principle and the data processing and conclude with measurement results.

  16. Nanoimprint of a 3D structure on an optical fiber for light wavefront manipulation.

    PubMed

    Calafiore, Giuseppe; Koshelev, Alexander; Allen, Frances I; Dhuey, Scott; Sassolini, Simone; Wong, Edward; Lum, Paul; Munechika, Keiko; Cabrini, Stefano

    2016-09-16

    Integration of complex photonic structures onto optical fiber facets enables powerful platforms with unprecedented optical functionalities. Conventional nanofabrication technologies, however, do not permit viable integration of complex photonic devices onto optical fibers owing to their low throughput and high cost. In this paper we report the fabrication of a three-dimensional structure achieved by direct nanoimprint lithography on the facet of an optical fiber. Nanoimprint processes and tools were specifically developed to enable a high lithographic accuracy and coaxial alignment of the optical device with respect to the fiber core. To demonstrate the capability of this new approach, a 3D beam splitter has been designed, imprinted and optically characterized. Scanning electron microscopy and optical measurements confirmed the good lithographic capabilities of the proposed approach as well as the desired optical performance of the imprinted structure. The inexpensive solution presented here should enable advancements in areas such as integrated optics and sensing, achieving enhanced portability and versatility of fiber optic components.

  17. Nanoimprint of a 3D structure on an optical fiber for light wavefront manipulation

    NASA Astrophysics Data System (ADS)

    Calafiore, Giuseppe; Koshelev, Alexander; Allen, Frances I.; Dhuey, Scott; Sassolini, Simone; Wong, Edward; Lum, Paul; Munechika, Keiko; Cabrini, Stefano

    2016-09-01

    Integration of complex photonic structures onto optical fiber facets enables powerful platforms with unprecedented optical functionalities. Conventional nanofabrication technologies, however, do not permit viable integration of complex photonic devices onto optical fibers owing to their low throughput and high cost. In this paper we report the fabrication of a three-dimensional structure achieved by direct nanoimprint lithography on the facet of an optical fiber. Nanoimprint processes and tools were specifically developed to enable a high lithographic accuracy and coaxial alignment of the optical device with respect to the fiber core. To demonstrate the capability of this new approach, a 3D beam splitter has been designed, imprinted and optically characterized. Scanning electron microscopy and optical measurements confirmed the good lithographic capabilities of the proposed approach as well as the desired optical performance of the imprinted structure. The inexpensive solution presented here should enable advancements in areas such as integrated optics and sensing, achieving enhanced portability and versatility of fiber optic components.

  18. 3D reconstruction and characterization of laser induced craters by in situ optical microscopy

    NASA Astrophysics Data System (ADS)

    Casal, A.; Cerrato, R.; Mateo, M. P.; Nicolas, G.

    2016-06-01

    A low-cost optical microscope was developed and coupled to an irradiation system in order to study the induced effects on material during a multipulse regime by an in situ visual inspection of the surface, in particular of the spot generated at different pulses. In the case of laser ablation, a reconstruction of the crater in 3D was made from the images of the sample surface taken during the irradiation process, and the subsequent profiles of ablated material were extracted. The implementation of this homemade optical device gives an added value to the irradiation system, providing information about morphology evolution of irradiated area when successive pulses are applied. In particular, the determination of ablation rates in real time can be especially useful for a better understanding and controlling of the ablation process in applications where removal of material is involved, such as laser cleaning and in-depth characterization of multilayered samples and diffusion processes. The validation of the developed microscope was made by a comparison with a commercial confocal microscope configured for the characterization of materials where similar results of crater depth and diameter were obtained for both systems.

  19. A Microscopic Optically Tracking Navigation System That Uses High-resolution 3D Computer Graphics.

    PubMed

    Yoshino, Masanori; Saito, Toki; Kin, Taichi; Nakagawa, Daichi; Nakatomi, Hirofumi; Oyama, Hiroshi; Saito, Nobuhito

    2015-01-01

    Three-dimensional (3D) computer graphics (CG) are useful for preoperative planning of neurosurgical operations. However, application of 3D CG to intraoperative navigation is not widespread because existing commercial operative navigation systems do not show 3D CG in sufficient detail. We have developed a microscopic optically tracking navigation system that uses high-resolution 3D CG. This article presents the technical details of our microscopic optically tracking navigation system. Our navigation system consists of three components: the operative microscope, registration, and the image display system. An optical tracker was attached to the microscope to monitor the position and attitude of the microscope in real time; point-pair registration was used to register the operation room coordinate system, and the image coordinate system; and the image display system showed the 3D CG image in the field-of-view of the microscope. Ten neurosurgeons (seven males, two females; mean age 32.9 years) participated in an experiment to assess the accuracy of this system using a phantom model. Accuracy of our system was compared with the commercial system. The 3D CG provided by the navigation system coincided well with the operative scene under the microscope. Target registration error for our system was 2.9 ± 1.9 mm. Our navigation system provides a clear image of the operation position and the surrounding structures. Systems like this may reduce intraoperative complications.

  20. A monthly quality assurance procedure for 3D surface imaging.

    PubMed

    Wooten, H Omar; Klein, Eric E; Gokhroo, Garima; Santanam, Lakshmi

    2010-12-21

    A procedure for periodic quality assurance of a video surface imaging system is introduced. AlignRT is a video camera-based patient localization system that captures and compares images of a patient's topography to a DICOM-formatted external contour, then calculates shifts required to accurately reposition the patient. This technical note describes the tools and methods implemented in our department to verify correct and accurate operation of the AlignRT hardware and software components. The procedure described is performed monthly and complements a daily calibration of the system.

  1. 3D printing optical watermark algorithms based on the combination of DWT and Fresnel transformation

    NASA Astrophysics Data System (ADS)

    Hu, Qi; Duan, Jin; Zhai, Di; Wang, LiNing

    2016-10-01

    With the continuous development of industrialization, 3D printing technology steps into individuals' lives gradually, however, the consequential security issue has become the urgent problem which is imminent. This paper proposes the 3D printing optical watermark algorithms based on the combination of DWT and Fresnel transformation and utilizes authorized key to restrict 3D model printing's permissions. Firstly, algorithms put 3D model into affine transform, and take the distance from the center of gravity to the vertex of 3D object in order to generate a one-dimensional discrete signal; then make this signal into wavelet transform and put the transformed coefficient into Fresnel transformation. Use math model to embed watermark information into it and finally generate 3D digital model with watermarking. This paper adopts VC++.NET and DIRECTX 9.0 SDK for combined developing and testing, and the results show that in fixed affine space, achieve the robustness in translation, revolving and proportion transforms of 3D model and better watermark-invisibility. The security and authorization of 3D model have been protected effectively.

  2. To 3D or Not to 3D, That Is the Question: Do 3D Surface Analyses Improve the Ecomorphological Power of the Distal Femur in Placental Mammals?

    PubMed Central

    Gould, Francois D. H.

    2014-01-01

    Improvements in three-dimensional imaging technologies have renewed interest in the study of functional and ecological morphology. Quantitative approaches to shape analysis are used increasingly to study form-function relationships. These methods are computationally intensive, technically demanding, and time-consuming, which may limit sampling potential. There have been few side-by-side comparisons of the effectiveness of such approaches relative to more traditional analyses using linear measurements and ratios. Morphological variation in the distal femur of mammals has been shown to reflect differences in locomotor modes across clades. Thus I tested whether a geometric morphometric analysis of surface shape was superior to a multivariate analysis of ratios for describing ecomorphological patterns in distal femoral variation. A sample of 164 mammalian specimens from 44 genera was assembled. Each genus was assigned to one of six locomotor categories. The same hypotheses were tested using two methods. Six linear measurements of the distal femur were taken with calipers, from which four ratios were calculated. A 3D model was generated with a laser scanner, and analyzed using three dimensional geometric morphometrics. Locomotor category significantly predicted variation in distal femoral morphology in both analyses. Effect size was larger in the geometric morphometric analysis than in the analysis of ratios. Ordination reveals a similar pattern with arboreal and cursorial taxa as extremes on a continuum of morphologies in both analyses. Discriminant functions calculated from the geometric morphometric analysis were more accurate than those calculated from ratios. Both analysis of ratios and geometric morphometric surface analysis reveal similar, biologically meaningful relationships between distal femoral shape and locomotor mode. The functional signal from the morphology is slightly higher in the geometric morphometric analysis. The practical costs of conducting these

  3. Brownian nanoimaging of interface dynamics and ligand-receptor binding at cell surfaces in 3-D.

    PubMed

    Kuznetsov, Igor R; Evans, Evan A

    2013-04-01

    We describe a method for nanoimaging interfacial dynamics and ligand-receptor binding at surfaces of live cells in 3-D. The imaging probe is a 1-μm diameter glass bead confined by a soft laser trap to create a "cloud" of fluctuating states. Using a facile on-line method of video image analysis, the probe displacements are reported at ~10 ms intervals with bare precisions (±SD) of 4-6 nm along the optical axis (elevation) and 2 nm in the transverse directions. We demonstrate how the Brownian distributions are analyzed to characterize the free energy potential of each small probe in 3-D taking into account the blur effect of its motions during CCD image capture. Then, using the approach to image interactions of a labeled probe with lamellae of leukocytic cells spreading on cover-glass substrates, we show that deformations of the soft distribution in probe elevations provide both a sensitive long-range sensor for defining the steric topography of a cell lamella and a fast telemetry for reporting rare events of probe binding with its surface receptors. Invoking established principles of Brownian physics and statistical thermodynamics, we describe an off-line method of super resolution that improves precision of probe separations from a non-reactive steric boundary to ~1 nm.

  4. Improving segmentation of 3D touching cell nuclei using flow tracking on surface meshes.

    PubMed

    Li, Gang; Guo, Lei

    2012-01-01

    Automatic segmentation of touching cell nuclei in 3D microscopy images is of great importance in bioimage informatics and computational biology. This paper presents a novel method for improving 3D touching cell nuclei segmentation. Given binary touching nuclei by the method in Li et al. (2007), our method herein consists of several steps: surface mesh reconstruction and curvature information estimation; direction field diffusion on surface meshes; flow tracking on surface meshes; and projection of surface mesh segmentation to volumetric images. The method is validated on both synthesised and real 3D touching cell nuclei images, demonstrating its validity and effectiveness.

  5. Nondestructive optical testing of 3D disperse systems with micro- and nano-particles

    NASA Astrophysics Data System (ADS)

    Bezrukova, Alexandra G.

    2005-04-01

    Nondestructive testing and analysis of three-dimensional (3D) disperse systems (DS) with micro- and nano-particles of different nature by complex of optical compatible methods can provide further progress in on-line control of water and air. The simultaneous analysis of 3D-DS by refractometry, absorbency, fluorescence and by different types of light scattering can help to elaborate the sensing elements for specific impurity control. In our research we have investigated by complex of optical methods different 3D-DS such as: proteins, nucleoproteids, lipoproteids, liposomes, viruses, virosomes, lipid emulsions, blood substitutes, latexes, liquid crystals, biological cells with various form and size (including bacterial cells), metallic powders, clays, kimberlites, zeolites, oils, crude oils, etc., and mixtures -- proteins with nucleic acids, liposomes and viruses, liquid crystals with surfactants, mixtures of clay with bacterial cells, samples of natural and water-supply waters, etc. This experience suggests that the set of optical parameters of so called second class is unique for each 3D-DS. In another words each DS can be characterized by n-dimensional vector in n-dimensional space of optical parameters. Mixtures can be considered as polycomponent and polymodal 3D-DS (such as natural water and air). Due to the fusion of various optical data it is possible to indicate by information statistical theory the inverse physical problem on the presence of impurities in mixtures (viruses, bacteria, oil, metallic particles, etc.), and in this case polymodality of particle size distribution is not an obstacle. Bank of optical data for 3D-DS is the base for analysis by information-statistical method.

  6. 3-D segmentation of retinal blood vessels in spectral-domain OCT volumes of the optic nerve head

    NASA Astrophysics Data System (ADS)

    Lee, Kyungmoo; Abràmoff, Michael D.; Niemeijer, Meindert; Garvin, Mona K.; Sonka, Milan

    2010-03-01

    Segmentation of retinal blood vessels can provide important information for detecting and tracking retinal vascular diseases including diabetic retinopathy, arterial hypertension, arteriosclerosis and retinopathy of prematurity (ROP). Many studies on 2-D segmentation of retinal blood vessels from a variety of medical images have been performed. However, 3-D segmentation of retinal blood vessels from spectral-domain optical coherence tomography (OCT) volumes, which is capable of providing geometrically accurate vessel models, to the best of our knowledge, has not been previously studied. The purpose of this study is to develop and evaluate a method that can automatically detect 3-D retinal blood vessels from spectral-domain OCT scans centered on the optic nerve head (ONH). The proposed method utilized a fast multiscale 3-D graph search to segment retinal surfaces as well as a triangular mesh-based 3-D graph search to detect retinal blood vessels. An experiment on 30 ONH-centered OCT scans (15 right eye scans and 15 left eye scans) from 15 subjects was performed, and the mean unsigned error in 3-D of the computer segmentations compared with the independent standard obtained from a retinal specialist was 3.4 +/- 2.5 voxels (0.10 +/- 0.07 mm).

  7. Design, Simulation and Optimisation of a Fibre-optic 3D Accelerometer

    NASA Astrophysics Data System (ADS)

    Yang, Zhen; Fang, Xiao-Yong; Zhou, Yan; Li, Ya-lin; Yuan, Jie; Cao, Mao-Sheng

    2013-07-01

    Using an inertia pendulum comprised of two prisms, flexible beams and an elastic flake, we present a novel fibre-optic 3D accelerometer design. The total reverse reflection of the cube-corner prism and the spectroscopic property of an orthogonal holographic grating enable the measurement of the two transverse components of the 3D acceleration simultaneously, while the longitudinal component can be determined from the elastic deformation of the flake. Due to optical interferometry, this sensor may provide a wider range, higher sensitivity and better resolving power than other accelerometers. Moreover, we use finite element analysis to study the performance and to optimise the structural design of the sensor.

  8. Multimodal photoacoustic and optical coherence tomography scanner using an all optical detection scheme for 3D morphological skin imaging.

    PubMed

    Zhang, Edward Z; Povazay, Boris; Laufer, Jan; Alex, Aneesh; Hofer, Bernd; Pedley, Barbara; Glittenberg, Carl; Treeby, Bradley; Cox, Ben; Beard, Paul; Drexler, Wolfgang

    2011-08-01

    A noninvasive, multimodal photoacoustic and optical coherence tomography (PAT/OCT) scanner for three-dimensional in vivo (3D) skin imaging is described. The system employs an integrated, all optical detection scheme for both modalities in backward mode utilizing a shared 2D optical scanner with a field-of-view of ~13 × 13 mm(2). The photoacoustic waves were detected using a Fabry Perot polymer film ultrasound sensor placed on the surface of the skin. The sensor is transparent in the spectral range 590-1200 nm. This permits the photoacoustic excitation beam (670-680 nm) and the OCT probe beam (1050 nm) to be transmitted through the sensor head and into the underlying tissue thus providing a backward mode imaging configuration. The respective OCT and PAT axial resolutions were 8 and 20 µm and the lateral resolutions were 18 and 50-100 µm. The system provides greater penetration depth than previous combined PA/OCT devices due to the longer wavelength of the OCT beam (1050 nm rather than 829-870 nm) and by operating in the tomographic rather than the optical resolution mode of photoacoustic imaging. Three-dimensional in vivo images of the vasculature and the surrounding tissue micro-morphology in murine and human skin were acquired. These studies demonstrated the complementary contrast and tissue information provided by each modality for high-resolution 3D imaging of vascular structures to depths of up to 5 mm. Potential applications include characterizing skin conditions such as tumors, vascular lesions, soft tissue damage such as burns and wounds, inflammatory conditions such as dermatitis and other superficial tissue abnormalities.

  9. Sub-millimeter resolution 3D optical imaging of living tissue using laminar optical tomography

    PubMed Central

    Hillman, Elizabeth M. C.; Burgess, Sean A.

    2009-01-01

    In-vivo imaging of optical contrast in living tissues can allow measurement of functional parameters such as blood oxygenation and detection of targeted and active fluorescent contrast agents. However, optical imaging must overcome the effects of light scattering, which limit the penetration depth and can affect quantitation and sensitivity. This article focuses on a technique for high-resolution, high-speed depth-resolved optical imaging of superficial living tissues called laminar optical tomography (LOT), which is capable of imaging absorbing and fluorescent contrast in living tissues to depths of 2–3 mm with 100–200 micron resolution. An overview of the advantages and challenges of in-vivo optical imaging is followed by a review of currently available techniques for high-resolution optical imaging of tissues. LOT is then described, including a description of the imaging system design and discussion of data analysis and image reconstruction approaches. Examples of recent applications of LOT are then provided and compared to other existing technologies. By measuring multiply-scattered light, Laminar Optical Tomography can probe beneath the surface of living tissues such as the skin and brain. PMID:19844595

  10. Metal-mesh based transparent electrode on a 3-D curved surface by electrohydrodynamic jet printing

    NASA Astrophysics Data System (ADS)

    Seong, Baekhoon; Yoo, Hyunwoong; Dat Nguyen, Vu; Jang, Yonghee; Ryu, Changkook; Byun, Doyoung

    2014-09-01

    Invisible Ag mesh transparent electrodes (TEs), with a width of 7 μm, were prepared on a curved glass surface by electrohydrodynamic (EHD) jet printing. With a 100 μm pitch, the EHD jet printed the Ag mesh on the convex glass which had a sheet resistance of 1.49 Ω/□. The printing speed was 30 cm s-1 using Ag ink, which had a 10 000 cPs viscosity and a 70 wt% Ag nanoparticle concentration. We further showed the performance of a 3-D transparent heater using the Ag mesh transparent electrode. The EHD jet printed an invisible Ag grid transparent electrode with good electrical and optical properties with promising applications on printed optoelectronic devices.

  11. Quantifying axis orientation in 3D using polarization-sensitive optical coherence tomography (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Liu, Chao; Black, Adam J.; Wang, Hui; Akkin, Taner

    2016-03-01

    The optic axis of birefringent tissues indicates the direction of structural anisotropy. Polarization-sensitive Optical Coherence Tomography (PS-OCT) can provide reflectivity contrast as well as retardance and optic axis orientation contrasts that originate from tissue birefringence. We introduce imaging 3D tissue anisotropy by using a single-camera and polarization-maintaining fiber (PMF) based PS-OCT, which utilizes normal and angled illuminations. Because environmental factors such as the movement of PMF and temperature fluctuations induce arbitrary phase changes, the optic axis orientation measurement has a time-varying offset. In order to measure the absolute axis orientation, we add a calibration path which dynamically provides the arbitrary offset to be subtracted from the relative axis orientation values. The axis orientation on the normal plane is the 2D projection of the fiber direction in 3D space. We propose to characterize the axis orientation in different planes (xy, xy' and x'y planes) by using normal and angled illuminations. This allows calculation of the polar angle that completes the orientation information in 3D. Polarization-based optical systems relying on one illumination angle measure the "apparent birefringence" that light encounters rather than the "true birefringence". Birefringence as a measure of anisotropy is quantified with the orientation information in 3D. The method and validation with a biological tissue are presented. The study can facilitate imaging and mapping the structural connections in anisotropic tissues including the brain.

  12. 3D-optical measurement system using a new vignetting aperture procedure

    NASA Astrophysics Data System (ADS)

    Hofbauer, Engelbert; Rascher, Rolf; Wühr, Konrad; Friedke, Felix; Stubenrauch, Thomas; Pastötter, Benjamin; Schleich, Sebastian; Zöcke, Christine

    2014-05-01

    A newly developed measuring procedure uses vignetting to evaluate angles and angle changes, independently from the measurement distance. Further on, the same procedure enables the transmission of a digital readout and therefore a better automation of the electronic signal evaluation, for use as an alignment telescope. The fully extended readout by a simple 3-D reflector will provide the user with a measurement result with six degrees of freedom. The vignetting field stop procedure will be described. Firstly, considering artificial vignetting, the theoretical basics from geometric-optical view are represented. Secondly, the natural vignetting with photometric effects will be considered. The distribution of intensity in the image plane light spot, the so-called V-SPOT, is analytically deduced as a function of differently measured variables. Intensity shifts within the V-Spot are examined independently from different effects by numeric simulation. On these basics, the theoretical research regarding accuracy, linearity as well as results in 2 dimensional surface reconstruction on precision optical mirrors and also three dimensional measurements in mechanical engineering are examined. Effects and deviations will be discussed. The project WiPoVi is sponsored by "Ingenieur Nachwuchs - Qualifizierung von Ingenieurnachwuchs an Fachhochschulen" by Bavarian State Ministry of Education, Science and the Arts.

  13. Monocular accommodation condition in 3D display types through geometrical optics

    NASA Astrophysics Data System (ADS)

    Kim, Sung-Kyu; Kim, Dong-Wook; Park, Min-Chul; Son, Jung-Young

    2007-09-01

    Eye fatigue or strain phenomenon in 3D display environment is a significant problem for 3D display commercialization. The 3D display systems like eyeglasses type stereoscopic or auto-stereoscopic multiview, Super Multi-View (SMV), and Multi-Focus (MF) displays are considered for detail calculation about satisfaction level of monocular accommodation by geometrical optics calculation means. A lens with fixed focal length is used for experimental verification about numerical calculation of monocular defocus effect caused by accommodation at three different depths. And the simulation and experiment results consistently show relatively high level satisfaction about monocular accommodation at MF display condition. Additionally, possibility of monocular depth perception, 3D effect, at monocular MF display is discussed.

  14. Flying triangulation--an optical 3D sensor for the motion-robust acquisition of complex objects.

    PubMed

    Ettl, Svenja; Arold, Oliver; Yang, Zheng; Häusler, Gerd

    2012-01-10

    Three-dimensional (3D) shape acquisition is difficult if an all-around measurement of an object is desired or if a relative motion between object and sensor is unavoidable. An optical sensor principle is presented-we call it "flying triangulation"-that enables a motion-robust acquisition of 3D surface topography. It combines a simple handheld sensor with sophisticated registration algorithms. An easy acquisition of complex objects is possible-just by freely hand-guiding the sensor around the object. Real-time feedback of the sequential measurement results enables a comfortable handling for the user. No tracking is necessary. In contrast to most other eligible sensors, the presented sensor generates 3D data from each single camera image.

  15. Review of three-dimensional (3D) surface imaging for oncoplastic, reconstructive and aesthetic breast surgery.

    PubMed

    O'Connell, Rachel L; Stevens, Roger J G; Harris, Paul A; Rusby, Jennifer E

    2015-08-01

    Three-dimensional surface imaging (3D-SI) is being marketed as a tool in aesthetic breast surgery. It has recently also been studied in the objective evaluation of cosmetic outcome of oncological procedures. The aim of this review is to summarise the use of 3D-SI in oncoplastic, reconstructive and aesthetic breast surgery. An extensive literature review was undertaken to identify published studies. Two reviewers independently screened all abstracts and selected relevant articles using specific inclusion criteria. Seventy two articles relating to 3D-SI for breast surgery were identified. These covered endpoints such as image acquisition, calculations and data obtainable, comparison of 3D and 2D imaging and clinical research applications of 3D-SI. The literature provides a favourable view of 3D-SI. However, evidence of its superiority over current methods of clinical decision making, surgical planning, communication and evaluation of outcome is required before it can be accepted into mainstream practice.

  16. Analysis of 3D-printed metal for rapid-prototyped reflective terahertz optics

    NASA Astrophysics Data System (ADS)

    Headland, Daniel; Withayachumnankul, Withawat; Webb, Michael; Ebendorff-Heidepriem, Heike; Luiten, Andre; Abbott, Derek

    2016-07-01

    We explore the potential of 3D metal printing to realize complex conductive terahertz devices. Factors impacting performance such as printing resolution, surface roughness, oxidation, and material loss are investigated via analytical, numerical, and experimental approaches. The high degree of control offered by a 3D-printed topology is exploited to realize a zone plate operating at 530 GHz. Reflection efficiency at this frequency is found to be over 90%. The high-performance of this preliminary device suggest that 3D metal printing can play a strong role in guided-wave and general beam control devices in the terahertz range.

  17. High resolution 3D dosimetry for microbeam radiation therapy using optical CT

    NASA Astrophysics Data System (ADS)

    McErlean, C.; Bräuer-Krisch, E.; Adamovics, J.; Leach, M. O.; Doran, S. J.

    2015-01-01

    Optical Computed Tomography (CT) is a promising technique for dosimetry of Microbeam Radiation Therapy (MRT), providing high resolution 3D dose maps. Here different MRT irradiation geometries are visualised showing the potential of Optical CT as a tool for future MRT trials. The Peak-to-Valley dose ratio (PVDR) is calculated to be 7 at a depth of 3mm in the radiochromic dosimeter PRESAGE®. This is significantly lower than predicted values and possible reasons for this are discussed.

  18. Simulation of light transport in scintillators based on 3D characterization of crystal surfaces.

    PubMed

    Roncali, Emilie; Cherry, Simon R

    2013-04-07

    In the development of positron emission tomography (PET) detectors, understanding and optimizing scintillator light collection is critical for achieving high performance, particularly when the design incorporates depth-of-interaction (DOI) encoding or time-of-flight information. Monte-Carlo simulations play an important role in guiding research in detector designs and popular software such as GATE now include models of light transport in scintillators. Although current simulation toolkits are able to provide accurate models of perfectly polished surfaces, they do not successfully predict light output for other surface finishes, for example those often used in DOI-encoding detectors. The lack of accuracy of those models mainly originates from a simplified description of rough surfaces as an ensemble of micro-facets determined by the distribution of their normal, typically a gaussian distribution. The user can specify the standard deviation of this distribution, but this parameter does not provide a full description of the surface reflectance properties. We propose a different approach based on 3D measurements of the surface using atomic force microscopy. Polished and rough (unpolished) crystals were scanned to compute the surface reflectance properties. The angular distributions of reflectance and reflected rays were computed and stored in look-up tables (LUTs). The LUTs account for the effect of incidence angle and were integrated in a light transport model. Crystals of different sizes were simulated with and without reflector. The simulated maximum light output and the light output as a function of DOI showed very good agreement with experimental characterization of the crystals, indicating that our approach provides an accurate model of polished and rough surfaces and could be used to predict light collection in scintillators. This model is based on a true 3D representation of the surface, makes no assumption about the surface and provides insight on the optical

  19. Simulation of light transport in scintillators based on 3D characterization of crystal surfaces

    NASA Astrophysics Data System (ADS)

    Roncali, Emilie; Cherry, Simon R.

    2013-04-01

    In the development of positron emission tomography (PET) detectors, understanding and optimizing scintillator light collection is critical for achieving high performance, particularly when the design incorporates depth-of-interaction (DOI) encoding or time-of-flight information. Monte-Carlo simulations play an important role in guiding research in detector designs and popular software such as GATE now include models of light transport in scintillators. Although current simulation toolkits are able to provide accurate models of perfectly polished surfaces, they do not successfully predict light output for other surface finishes, for example those often used in DOI-encoding detectors. The lack of accuracy of those models mainly originates from a simplified description of rough surfaces as an ensemble of micro-facets determined by the distribution of their normal, typically a Gaussian distribution. The user can specify the standard deviation of this distribution, but this parameter does not provide a full description of the surface reflectance properties. We propose a different approach based on 3D measurements of the surface using atomic force microscopy. Polished and rough (unpolished) crystals were scanned to compute the surface reflectance properties. The angular distributions of reflectance and reflected rays were computed and stored in look-up tables (LUTs). The LUTs account for the effect of incidence angle and were integrated in a light transport model. Crystals of different sizes were simulated with and without reflector. The simulated maximum light output and the light output as a function of DOI showed very good agreement with experimental characterization of the crystals, indicating that our approach provides an accurate model of polished and rough surfaces and could be used to predict light collection in scintillators. This model is based on a true 3D representation of the surface, makes no assumption about the surface and provides insight on the optical

  20. Variational approach to reconstruct surface from sparse and nonparallel contours in freehand 3D ultrasound imaging

    NASA Astrophysics Data System (ADS)

    Deng, Shuangcheng; Jiang, Lipei; Cao, Yingyu; Zhang, Junwen; Zheng, Haiyang

    2012-01-01

    The 3D reconstruction for freehand 3D ultrasound is a challenging issue because the recorded B-scans are not only sparse, but also non-parallel (actually they may intersect each other). Conventional volume reconstruction methods can't reconstruct sparse data efficiently while not introducing geometrical artifacts, and conventional surface reconstruction methods can't reconstruct surfaces from contours that are arbitrarily oriented in 3D space. We developed a new surface reconstruction method for freehand 3D ultrasound. It is based on variational implicit function which is presented by Greg Turk for shape transformation. In the new method, we first constructed on- & off-surface constraints from the segmented contours of all recorded B-scans, then used a variational interpolation technique to get a single implicit function in 3D. Finally, the implicit function was evaluated to extract the zero-valued surface as reconstruction result. Two experiment was conducted to assess our variational surface reconstruction method, and the experiment results have shown that the new method is capable of reconstructing surface smoothly from sparse contours which can be arbitrarily oriented in 3D space.

  1. Optically directed molecular transport and 3D isoelectric positioning of amphoteric biomolecules

    PubMed Central

    Hafeman, Dean G.; Harkins, James B.; Witkowski, Charles E.; Lewis, Nathan S.; Warmack, Robert J.; Brown, Gilbert M.; Thundat, Thomas

    2006-01-01

    We demonstrate the formation of charged molecular packets and their transport within optically created electrical force-field traps in a pH-buffered electrolyte. We call this process photoelectrophoretic localization and transport (PELT). The electrolyte is in contact with a photoconductive semiconductor electrode and a counterelectrode that are connected through an external circuit. A light beam directed to coordinates on the photoconductive electrode surface produces a photocurrent within the circuit and electrolyte. Within the electrolyte, the photocurrent creates localized force-field traps centered at the illuminated coordinates. Charged molecules, including polypeptides and proteins, electrophoretically accumulate into the traps and subsequently can be transported in the electrolyte by moving the traps over the photoconductive electrode in response to movement of the light beam. The molecules in a single trap can be divided into aliquots, and the aliquots can be directed along multiple routes simultaneously by using multiple light beams. This photoelectrophoretic transport of charged molecules by PELT resembles the electrostatic transport of electrons within force-field wells of solid-state charge-coupled devices. The molecules, however, travel in a liquid electrolyte rather than a solid. Furthermore, we have used PELT to position amphoteric biomolecules in three dimensions. A 3D pH gradient was created in an electrolyte medium by controlling the illumination position on a photoconductive anode where protons were generated electrolytically. Photoelectrophoretic transport of amphoteric molecules through the pH gradient resulted in accumulation of the molecules at their apparent 3D isoelectric coordinates in the medium. PMID:16618926

  2. Semiautomatic approaches to account for 3-D distortion of the electric field from local, near-surface structures in 3-D resistivity inversions of 3-D regional magnetotelluric data

    USGS Publications Warehouse

    Rodriguez, Brian D.

    2017-03-31

    This report summarizes the results of three-dimensional (3-D) resistivity inversion simulations that were performed to account for local 3-D distortion of the electric field in the presence of 3-D regional structure, without any a priori information on the actual 3-D distribution of the known subsurface geology. The methodology used a 3-D geologic model to create a 3-D resistivity forward (“known”) model that depicted the subsurface resistivity structure expected for the input geologic configuration. The calculated magnetotelluric response of the modeled resistivity structure was assumed to represent observed magnetotelluric data and was subsequently used as input into a 3-D resistivity inverse model that used an iterative 3-D algorithm to estimate 3-D distortions without any a priori geologic information. A publicly available inversion code, WSINV3DMT, was used for all of the simulated inversions, initially using the default parameters, and subsequently using adjusted inversion parameters. A semiautomatic approach of accounting for the static shift using various selections of the highest frequencies and initial models was also tested. The resulting 3-D resistivity inversion simulation was compared to the “known” model and the results evaluated. The inversion approach that produced the lowest misfit to the various local 3-D distortions was an inversion that employed an initial model volume resistivity that was nearest to the maximum resistivities in the near-surface layer.

  3. Image detection of inner wall surface of holes in metal sheets through polarization using a 3D TV monitor

    NASA Astrophysics Data System (ADS)

    Suzuki, Takamasa; Nakano, Katsunori; Muramatsu, Shogo; Oitate, Toshiro

    2012-11-01

    We propose an effective technique for optically detecting images of the inner hole-surface of a hole (hereafter, referred to as the hole-surface) using the polarization property of a 3D television (TV) monitor. The polarized light emitted by the TV monitor illuminates the hole-surfaces present in the test target placed on the screen of the monitor. When the polarizer placed in front of a camera lens is adjusted such that the camera captures a dark image for the transmitted light, only the highlighted hole-surfaces are visible in the captured image.

  4. Edge structure preserving 3D image denoising by local surface approximation.

    PubMed

    Qiu, Peihua; Mukherjee, Partha Sarathi

    2012-08-01

    In various applications, including magnetic resonance imaging (MRI) and functional MRI (fMRI), 3D images are becoming increasingly popular. To improve the reliability of subsequent image analyses, 3D image denoising is often a necessary preprocessing step, which is the focus of the current paper. In the literature, most existing image denoising procedures are for 2D images. Their direct extensions to 3D cases generally cannot handle 3D images efficiently because the structure of a typical 3D image is substantially more complicated than that of a typical 2D image. For instance, edge locations are surfaces in 3D cases which would be much more challenging to handle compared to edge curves in 2D cases. We propose a novel 3D image denoising procedure in this paper, based on local approximation of the edge surfaces using a set of surface templates. An important property of this method is that it can preserve edges and major edge structures (e.g., intersections of two edge surfaces and pointed corners). Numerical studies show that it works well in various applications.

  5. A physical model eye with 3D resolution test targets for optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Hu, Zhixiong; Liu, Wenli; Hong, Baoyu; Hao, Bingtao; Wang, Lele; Li, Jiao

    2014-09-01

    Optical coherence tomography (OCT) has been widely employed as non-invasive 3D imaging diagnostic instrument, particularly in the field of ophthalmology. Although OCT has been approved for use in clinic in USA, Europe and Asia, international standardization of this technology is still in progress. Validation of OCT imaging capabilities is considered extremely important to ensure its effective use in clinical diagnoses. Phantom with appropriate test targets can assist evaluate and calibrate imaging performance of OCT at both installation and throughout lifetime of the instrument. In this paper, we design and fabricate a physical model eye with 3D resolution test targets to characterize OCT imaging performance. The model eye was fabricated with transparent resin to simulate realistic ophthalmic testing environment, and most key optical elements including cornea, lens and vitreous body were realized. The test targets which mimic USAF 1951 test chart were fabricated on the fundus of the model eye by 3D printing technology. Differing from traditional two dimensional USAF 1951 test chart, a group of patterns which have different thickness in depth were fabricated. By measuring the 3D test targets, axial resolution as well as lateral resolution of an OCT system can be evaluated at the same time with this model eye. To investigate this specialized model eye, it was measured by a scientific spectral domain OCT instrument and a clinical OCT system respectively. The results demonstrate that the model eye with 3D resolution test targets have the potential of qualitatively and quantitatively validating the performance of OCT systems.

  6. 3D surface topography study of the biofunctionalized nanocrystalline Ti-6Zr-4Nb/Ca-P

    SciTech Connect

    Jakubowicz, J.; Adamek, G.; Jurczyk, M.U.; Jurczyk, M.

    2012-08-15

    In this work surface of the sintered Ti-6Zr-4Nb nanocrystalline alloy was electrochemically biofunctionalized. The porous surface was produced by anodic oxidation in 1 M H{sub 3}PO{sub 4} + 2%HF electrolyte at 10 V for 30 min. Next the calcium-phosphate (Ca-P) layer was deposited, onto the formed porous surface, using cathodic potential - 5 V kept for 60 min in 0.042 M Ca(NO{sub 3}){sub 2} + 0.025 M (NH{sub 4}){sub 2}HPO{sub 4} + 0.1 M HCl electrolyte. The deposited Ca-P layer anchored in the pores. The biofunctionalized surface was studied by XRD, SEM and EDS. In vitro tests culture of normal human osteoblast (NHOst) cells showed very good cells proliferation, colonization and multilayering. Using optical profiler, roughness and hybrid 3D surface topography parameters were estimated. Correlation between surface composition, morphology, roughness and biocompatibility results was done. It has been shown by us that surface with appropriate chemical composition and topography, after combined electrochemical anodic and cathodic surface treatment, supports osteoblast adhesion and proliferation. 3D topography measurements using optical profiler play a key role in the biomaterials surface analysis. - Highlights: Black-Right-Pointing-Pointer Nanocrystalline Ti-6Zr-4Nb/Ca-P material was produced for hard tissue implant applications. Black-Right-Pointing-Pointer Calcium-phosphate results in surface biofunctionalization. Black-Right-Pointing-Pointer The biofunctionalized surface shows good in-vitro behavior.

  7. Alignment of 3-D optical coherence tomography scans to correct eye movement using a particle filtering.

    PubMed

    Xu, Juan; Ishikawa, Hiroshi; Wollstein, Gadi; Kagemann, Larry; Schuman, Joel S

    2012-07-01

    Eye movement artifacts occurring during 3-D optical coherence tomography (OCT) scanning is a well-recognized problem that may adversely affect image analysis and interpretation. A particle filtering algorithm is presented in this paper to correct motion in a 3-D dataset by considering eye movement as a target tracking problem in a dynamic system. The proposed particle filtering algorithm is an independent 3-D alignment approach, which does not rely on any reference image. 3-D OCT data is considered as a dynamic system, while the location of each A-scan is represented by the state space. A particle set is used to approximate the probability density of the state in the dynamic system. The state of the system is updated frame by frame to detect A-scan movement. The proposed method was applied on both simulated data for objective evaluation and experimental data for subjective evaluation. The sensitivity and specificity of the x-movement detection were 98.85% and 99.43%, respectively, in the simulated data. For the experimental data (74 3-D OCT images), all the images were improved after z-alignment, while 81.1% images were improved after x-alignment. The proposed algorithm is an efficient way to align 3-D OCT volume data and correct the eye movement without using references.

  8. Towards non-invasive 3D hepatotoxicity assays with optical coherence phase microscopy

    NASA Astrophysics Data System (ADS)

    Nelson, Leonard J.; Koulovasilopoulos, Andreas; Treskes, Philipp; Hayes, Peter C.; Plevris, John N.; Bagnaninchi, Pierre O.

    2015-03-01

    Three-dimensional tissue-engineered models are increasingly recognised as more physiologically-relevant than standard 2D cell culture for pre-clinical drug toxicity testing. However, many types of conventional toxicity assays are incompatible with dense 3D tissues. This study investigated the use of optical coherence phase microscopy (OCPM) as a novel approach to assess cell death in 3D tissue culture. For 3D micro-spheroid formation Human hepatic C3A cells were encapsulated in hyaluronic acid gels and cultured in 100μl MEME/10%FBS in 96-well plates. After spheroid formation the 3D liver constructs were exposed to acetaminophen on culture day 8. Acetaminophen hepatotoxicity in 3D cultures was evaluated using standard biochemical assays. An inverted OCPM in common path configuration was developed with a Callisto OCT engine (Thorlabs), centred at 930nm and a custom scanning head. Intensity data were used to perform in-depth microstructural imaging. In addition, phase fluctuations were measured by collecting several successive B scans at the same location, and statistics on the first time derivative of the phase, i.e. time fluctuations, were analysed over the acquisition time interval to retrieve overall cell viability. OCPM intensity (cell cluster size) and phase fluctuation statistics were directly compared with biochemical assays. In this study, we investigated optical coherence phase tomography to assess cell death in a 3d liver model after exposure to a prototypical hepatotoxin, acetaminophen. We showed that OCPM has the potential to assess noninvasively and label-free drug toxicity in 3D tissue models.

  9. Investigation of inclined dual-fiber optical tweezers for 3D manipulation and force sensing.

    PubMed

    Liu, Yuxiang; Yu, Miao

    2009-08-03

    Optical tweezers provide a versatile tool in biological and physical researches. Optical tweezers based on optical fibers are more flexible and ready to be integrated when compared with those based on microscope objectives. In this paper, the three-dimensional (3D) trapping ability of an inclined dual-fiber optical tweezers is demonstrated. The trapping efficiency with respect to displacement is experimentally calibrated along two dimensions. The system is studied numerically using a modified ray-optics model. The spring constants obtained in the experiment are predicted by simulations. It is found both experimentally and numerically that there is a critical value for the fiber inclination angle to retain the 3D trapping ability. The inclined dual-fiber optical tweezers are demonstrated to be more robust to z-axis misalignment than the counter-propagating fiber optical tweezers, which is a special case of th former when the fiber inclination angle is 90 masculine. This inclined dual-fiber optical tweezers can serve as both a manipulator and a force sensor in integrated systems, such as microfluidic systems and lab-on-a-chip systems.

  10. Optically coupled 3D common memory with GaAs on Si structure

    NASA Astrophysics Data System (ADS)

    Hirose, M.; Takata, H.; Koyanagi, M.

    1991-02-01

    An ultra fast data transfer speed is demonstrated for a novel three-dimensional (3D) Static Random Access Memory (SRAM) consisting of multilayer silicon LSI chips on which GaAs LEDs and photodetectors are monolithically integrated for vertical optical interconnections. A unique feature of this system is the capability of parallel data transfer from one memory layer to the upper and lower memory layers by the optical interconnections. The results of static and dynamic simulations of the optically coupled 3D common memory have indicated that a block of 512 bits data can be transferred through four memory layers within 16 nsec. This is an equivalent data transfer speed of 128 Gbits/sec/layer.

  11. Performance of an improved first generation optical CT scanner for 3D dosimetry.

    PubMed

    Qian, Xin; Adamovics, John; Wuu, Cheng-Shie

    2013-12-21

    Performance analysis of a modified 3D dosimetry optical scanner based on the first generation optical CT scanner OCTOPUS is presented. The system consists of PRESAGE dosimeters, the modified 3D scanner, and a new developed in-house user control panel written in Labview program which provides more flexibility to optimize mechanical control and data acquisition technique. The total scanning time has been significantly reduced from initial 8 h to ∼2 h by using the modified scanner. The functional performance of the modified scanner has been evaluated in terms of the mechanical integrity uncertainty of the data acquisition process. Optical density distribution comparison between the modified scanner, OCTOPUS and the treatment plan system has been studied. It has been demonstrated that the agreement between the modified scanner and treatment plans is comparable with that between the OCTOPUS and treatment plans.

  12. Performance of an improved first generation optical CT scanner for 3D dosimetry

    NASA Astrophysics Data System (ADS)

    Qian, Xin; Adamovics, John; Wuu, Cheng-Shie

    2013-12-01

    Performance analysis of a modified 3D dosimetry optical scanner based on the first generation optical CT scanner OCTOPUS is presented. The system consists of PRESAGE™ dosimeters, the modified 3D scanner, and a new developed in-house user control panel written in Labview program which provides more flexibility to optimize mechanical control and data acquisition technique. The total scanning time has been significantly reduced from initial 8 h to ∼2 h by using the modified scanner. The functional performance of the modified scanner has been evaluated in terms of the mechanical integrity uncertainty of the data acquisition process. Optical density distribution comparison between the modified scanner, OCTOPUS and the treatment plan system has been studied. It has been demonstrated that the agreement between the modified scanner and treatment plans is comparable with that between the OCTOPUS and treatment plans.

  13. Virtual touch 3D interactive system for autostereoscopic display with embedded optical sensor

    NASA Astrophysics Data System (ADS)

    Huang, Yi-Pai; Wang, Guo-Zhen; Ma, Ming-Ching; Tung, Shang-Yu; Huang, Shu-Yi; Tseng, Hung-Wei; Kuo, Chung-Hong; Li, Chun-Huai

    2011-06-01

    The traidational 3D interactive sysetm which uses CCD camera to capture image is difficult to operate on near range for mobile applications.Therefore, 3D interactive display with embedded optical sensor was proposed. Based on optical sensor based system, we proposed four different methods to support differenct functions. T mark algorithm can obtain 5- axis information (x, y, z,θ, and φ)of LED no matter where LED was vertical or inclined to panel and whatever it rotated. Sequential mark algorithm and color filter based algorithm can support mulit-user. Finally, bare finger touch system with sequential illuminator can achieve to interact with auto-stereoscopic images by bare finger. Furthermore, the proposed methods were verified on a 4-inch panel with embedded optical sensors.

  14. Remote artificial eyes using micro-optical circuit for long-distance 3D imaging perception.

    PubMed

    Thammawongsa, Nopparat; Yupapin, Preecha P

    2016-01-01

    A small-scale optical device incorporated with an optical nano-antenna is designed to operate as the remote artificial eye using a tiny conjugate mirror. A basic device known as a conjugate mirror can be formed using the artificial eye device, the partially reflected light intensities from input source are interfered and the 3D whispering gallery modes formed within the ring centers, which can be modulated and propagated to the object. The image pixel is obtained at the center ring and linked with the optic nerve in the remote area via the nano-antenna, which is useful for blind people.

  15. Optical design of wavelength selective CPVT system with 3D/2D hybrid concentration

    NASA Astrophysics Data System (ADS)

    Ahmad, N.; Ijiro, T.; Yamada, N.; Kawaguchi, T.; Maemura, T.; Ohashi, H.

    2012-10-01

    Optical design of a concentrating photovoltaic/thermal (CPVT) system is carried out. Using wavelength-selective optics, the system demonstrates 3-D concentration onto a solar cell and 2-D concentration onto a thermal receiver. Characteristics of the two types of concentrator systems are examined with ray-tracing analysis. The first system is a glazed mirror-based concentrator system mounted on a 2-axis pedestal tracker. The size of the secondary optical element is minimized to decrease the cost of the system, and it has a wavelength-selective function for performing 3-D concentration onto a solar cell and 2-D concentration onto a thermal receiver. The second system is a non-glazed beamdown concentrator system containing parabolic mirrors in the lower part. The beam-down selective mirror performs 3-D concentration onto a solar cell placed above the beam-down selective mirror, and 2-D concentration down to a thermal receiver placed at the bottom level. The system is mounted on a two-axis carousel tracker. A parametric study is performed for those systems with different geometrical 2-D/3-D concentration ratios. Wavelength-selective optics such as hot/cold mirrors and spectrum-splitting technologies are taken into account in the analysis. Results show reduced heat load on the solar cell and increased total system efficiency compared to a non-selective CPV system. Requirements for the wavelength-selective properties are elucidated. It is also shown that the hybrid concept with 2-D concentration onto a thermal receiver and 3-D concentration onto a solar cell has an advantageous geometry because of the high total system efficiency and compatibility with the piping arrangement of the thermal receiver.

  16. Combined scanning probe nanotomography and optical microspectroscopy: a correlative technique for 3D characterization of nanomaterials.

    PubMed

    Mochalov, Konstantin E; Efimov, Anton E; Bobrovsky, Alexey; Agapov, Igor I; Chistyakov, Anton A; Oleinikov, Vladimir; Sukhanova, Alyona; Nabiev, Igor

    2013-10-22

    Combination of 3D structural analysis with optical characterization of the same sample area on the nanoscale is a highly demanded approach in nanophotonics, materials science, and quality control of nanomaterial. We have developed a correlative microscopy technique where the 3D structure of the sample is reconstructed on the nanoscale by means of a "slice-and-view" combination of ultramicrotomy and scanning probe microscopy (scanning probe nanotomography, SPNT), and its optical characteristics are analyzed using microspectroscopy. This approach has been used to determine the direct quantitative relationship of the 3D structural characteristics of nanovolumes of materials with their microscopic optical properties. This technique has been applied to 3D structural and optical characterization of a hybrid material consisting of cholesteric liquid crystals doped with fluorescent quantum dots (QDs) that can be used for photochemical patterning and image recording through the changes in the dissymmetry factor of the circular polarization of QD emission. The differences in the polarization images and fluorescent spectra of this hybrid material have proved to be correlated with the arrangement of the areas of homogeneous distribution and heterogeneous clustering of QDs. The reconstruction of the 3D nanostructure of the liquid crystal matrix in the areas of homogeneous QDs distribution has shown that QDs do not perturb the periodic planar texture of the cholesteric liquid crystal matrix, whereas QD clusters do perturb it. The combined microspectroscopy-nanotomography technique will be important for evaluating the effects of nanoparticles on the structural organization of organic and liquid crystal matrices and biomedical materials, as well as quality control of nanotechnology fabrication processes and products.

  17. In situ fabrication of 3D Ag@ZnO nanostructures for microfluidic surface-enhanced Raman scattering systems.

    PubMed

    Xie, Yuliang; Yang, Shikuan; Mao, Zhangming; Li, Peng; Zhao, Chenglong; Cohick, Zane; Huang, Po-Hsun; Huang, Tony Jun

    2014-12-23

    In this work, we develop an in situ method to grow highly controllable, sensitive, three-dimensional (3D) surface-enhanced Raman scattering (SERS) substrates via an optothermal effect within microfluidic devices. Implementing this approach, we fabricate SERS substrates composed of Ag@ZnO structures at prescribed locations inside microfluidic channels, sites within which current fabrication of SERS structures has been arduous. Conveniently, properties of the 3D Ag@ZnO nanostructures such as length, packing density, and coverage can also be adjusted by tuning laser irradiation parameters. After exploring the fabrication of the 3D nanostructures, we demonstrate a SERS enhancement factor of up to ∼2×10(6) and investigate the optical properties of the 3D Ag@ZnO structures through finite-difference time-domain simulations. To illustrate the potential value of our technique, low concentrations of biomolecules in the liquid state are detected. Moreover, an integrated cell-trapping function of the 3D Ag@ZnO structures records the surface chemical fingerprint of a living cell. Overall, our optothermal-effect-based fabrication technique offers an effective combination of microfluidics with SERS, resolving problems associated with the fabrication of SERS substrates in microfluidic channels. With its advantages in functionality, simplicity, and sensitivity, the microfluidic-SERS platform presented should be valuable in many biological, biochemical, and biomedical applications.

  18. In Situ Fabrication of 3D Ag@ZnO Nanostructures for Microfluidic Surface-Enhanced Raman Scattering Systems

    PubMed Central

    2015-01-01

    In this work, we develop an in situ method to grow highly controllable, sensitive, three-dimensional (3D) surface-enhanced Raman scattering (SERS) substrates via an optothermal effect within microfluidic devices. Implementing this approach, we fabricate SERS substrates composed of Ag@ZnO structures at prescribed locations inside microfluidic channels, sites within which current fabrication of SERS structures has been arduous. Conveniently, properties of the 3D Ag@ZnO nanostructures such as length, packing density, and coverage can also be adjusted by tuning laser irradiation parameters. After exploring the fabrication of the 3D nanostructures, we demonstrate a SERS enhancement factor of up to ∼2 × 106 and investigate the optical properties of the 3D Ag@ZnO structures through finite-difference time-domain simulations. To illustrate the potential value of our technique, low concentrations of biomolecules in the liquid state are detected. Moreover, an integrated cell-trapping function of the 3D Ag@ZnO structures records the surface chemical fingerprint of a living cell. Overall, our optothermal-effect-based fabrication technique offers an effective combination of microfluidics with SERS, resolving problems associated with the fabrication of SERS substrates in microfluidic channels. With its advantages in functionality, simplicity, and sensitivity, the microfluidic-SERS platform presented should be valuable in many biological, biochemical, and biomedical applications. PMID:25402207

  19. 3D printing of tissue-simulating phantoms for calibration of biomedical optical devices

    NASA Astrophysics Data System (ADS)

    Zhao, Zuhua; Zhou, Ximing; Shen, Shuwei; Liu, Guangli; Yuan, Li; Meng, Yuquan; Lv, Xiang; Shao, Pengfei; Dong, Erbao; Xu, Ronald X.

    2016-10-01

    Clinical utility of many biomedical optical devices is limited by the lack of effective and traceable calibration methods. Optical phantoms that simulate biological tissues used for optical device calibration have been explored. However, these phantoms can hardly simulate both structural and optical properties of multi-layered biological tissue. To address this limitation, we develop a 3D printing production line that integrates spin coating, light-cured 3D printing and Fused Deposition Modeling (FDM) for freeform fabrication of optical phantoms with mechanical and optical heterogeneities. With the gel wax Polydimethylsiloxane (PDMS), and colorless light-curable ink as matrix materials, titanium dioxide (TiO2) powder as the scattering ingredient, graphite powder and black carbon as the absorption ingredient, a multilayer phantom with high-precision is fabricated. The absorption and scattering coefficients of each layer are measured by a double integrating sphere system. The results demonstrate that the system has the potential to fabricate reliable tissue-simulating phantoms to calibrate optical imaging devices.

  20. Mapping 3D fiber orientation in tissue using dual-angle optical polarization tractography

    PubMed Central

    Wang, Y.; Ravanfar, M.; Zhang, K.; Duan, D.; Yao, G.

    2016-01-01

    Optical polarization tractography (OPT) has recently been applied to map fiber organization in the heart, skeletal muscle, and arterial vessel wall with high resolution. The fiber orientation measured in OPT represents the 2D projected fiber angle in a plane that is perpendicular to the incident light. We report here a dual-angle extension of the OPT technology to measure the actual 3D fiber orientation in tissue. This method was first verified by imaging the murine extensor digitorum muscle placed at various known orientations in space. The accuracy of the method was further studied by analyzing the 3D fiber orientation of the mouse tibialis anterior muscle. Finally we showed that dual-angle OPT successfully revealed the unique 3D “arcade” fiber structure in the bovine articular cartilage. PMID:27867698

  1. Mapping 3D fiber orientation in tissue using dual-angle optical polarization tractography.

    PubMed

    Wang, Y; Ravanfar, M; Zhang, K; Duan, D; Yao, G

    2016-10-01

    Optical polarization tractography (OPT) has recently been applied to map fiber organization in the heart, skeletal muscle, and arterial vessel wall with high resolution. The fiber orientation measured in OPT represents the 2D projected fiber angle in a plane that is perpendicular to the incident light. We report here a dual-angle extension of the OPT technology to measure the actual 3D fiber orientation in tissue. This method was first verified by imaging the murine extensor digitorum muscle placed at various known orientations in space. The accuracy of the method was further studied by analyzing the 3D fiber orientation of the mouse tibialis anterior muscle. Finally we showed that dual-angle OPT successfully revealed the unique 3D "arcade" fiber structure in the bovine articular cartilage.

  2. Mesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography.

    PubMed

    Chen, Lingling; Alexandrov, Yuriy; Kumar, Sunil; Andrews, Natalie; Dallman, Margaret J; French, Paul M W; McGinty, James

    2015-04-01

    We describe an angular multiplexed imaging technique for 3-D in vivo cell tracking of sparse cell distributions and optical projection tomography (OPT) with superior time-lapse resolution and a significantly reduced light dose compared to volumetric time-lapse techniques. We demonstrate that using dual axis OPT, where two images are acquired simultaneously at different projection angles, can enable localization and tracking of features in 3-D with a time resolution equal to the camera frame rate. This is achieved with a 200x reduction in light dose compared to an equivalent volumetric time-lapse single camera OPT acquisition with 200 projection angles. We demonstrate the application of this technique to mapping the 3-D neutrophil migration pattern observed over ~25.5 minutes in a live 2 day post-fertilisation transgenic LysC:GFP zebrafish embryo following a tail wound.

  3. Writing of 3D optical integrated circuits with ultrashort laser pulses in the presence of strong spherical aberration

    NASA Astrophysics Data System (ADS)

    Bukharin, M. A.; Skryabin, N. N.; Khudyakov, D. V.; Vartapetov, S. K.

    2016-09-01

    A novel technique was proposed for 3D femtosecond writing of waveguides and optical integrated circuits in the presence of strong spherical aberration, caused by inscription at significantly different depth under the surface of optical glasses and crystals. Strong negative effect of spherical aberration and related asymmetry of created structures was reduced due to transition to the cumulative thermal regime of femtosecond interaction with the material. The differences in the influence of spherical aberration effect in a broad depth range (larger than 200 µm) was compensated by dynamic adjustment of laser pulse energy during the process of waveguides recording. The presented approach has been experimentally implemented in fused silica. Obtained results can be used in production of a broad class of femtosecond written three-dimensional integrated optical systems, inscripted at non-optimal (for focusing lens) optical depth or in significantly extended range of depths.

  4. Wave optics theory and 3-D deconvolution for the light field microscope.

    PubMed

    Broxton, Michael; Grosenick, Logan; Yang, Samuel; Cohen, Noy; Andalman, Aaron; Deisseroth, Karl; Levoy, Marc

    2013-10-21

    Light field microscopy is a new technique for high-speed volumetric imaging of weakly scattering or fluorescent specimens. It employs an array of microlenses to trade off spatial resolution against angular resolution, thereby allowing a 4-D light field to be captured using a single photographic exposure without the need for scanning. The recorded light field can then be used to computationally reconstruct a full volume. In this paper, we present an optical model for light field microscopy based on wave optics, instead of previously reported ray optics models. We also present a 3-D deconvolution method for light field microscopy that is able to reconstruct volumes at higher spatial resolution, and with better optical sectioning, than previously reported. To accomplish this, we take advantage of the dense spatio-angular sampling provided by a microlens array at axial positions away from the native object plane. This dense sampling permits us to decode aliasing present in the light field to reconstruct high-frequency information. We formulate our method as an inverse problem for reconstructing the 3-D volume, which we solve using a GPU-accelerated iterative algorithm. Theoretical limits on the depth-dependent lateral resolution of the reconstructed volumes are derived. We show that these limits are in good agreement with experimental results on a standard USAF 1951 resolution target. Finally, we present 3-D reconstructions of pollen grains that demonstrate the improvements in fidelity made possible by our method.

  5. Wave optics theory and 3-D deconvolution for the light field microscope

    PubMed Central

    Broxton, Michael; Grosenick, Logan; Yang, Samuel; Cohen, Noy; Andalman, Aaron; Deisseroth, Karl; Levoy, Marc

    2013-01-01

    Light field microscopy is a new technique for high-speed volumetric imaging of weakly scattering or fluorescent specimens. It employs an array of microlenses to trade off spatial resolution against angular resolution, thereby allowing a 4-D light field to be captured using a single photographic exposure without the need for scanning. The recorded light field can then be used to computationally reconstruct a full volume. In this paper, we present an optical model for light field microscopy based on wave optics, instead of previously reported ray optics models. We also present a 3-D deconvolution method for light field microscopy that is able to reconstruct volumes at higher spatial resolution, and with better optical sectioning, than previously reported. To accomplish this, we take advantage of the dense spatio-angular sampling provided by a microlens array at axial positions away from the native object plane. This dense sampling permits us to decode aliasing present in the light field to reconstruct high-frequency information. We formulate our method as an inverse problem for reconstructing the 3-D volume, which we solve using a GPU-accelerated iterative algorithm. Theoretical limits on the depth-dependent lateral resolution of the reconstructed volumes are derived. We show that these limits are in good agreement with experimental results on a standard USAF 1951 resolution target. Finally, we present 3-D reconstructions of pollen grains that demonstrate the improvements in fidelity made possible by our method. PMID:24150383

  6. 3D printing method for freeform fabrication of optical phantoms simulating heterogeneous biological tissue

    NASA Astrophysics Data System (ADS)

    Wang, Minjie; Shen, Shuwei; Yang, Jie; Dong, Erbao; Xu, Ronald

    2014-03-01

    The performance of biomedical optical imaging devices heavily relies on appropriate calibration. However, many of existing calibration phantoms for biomedical optical devices are based on homogenous materials without considering the multi-layer heterogeneous structures observed in biological tissue. Using such a phantom for optical calibration may result in measurement bias. To overcome this problem, we propose a 3D printing method for freeform fabrication of tissue simulating phantoms with multilayer heterogeneous structure. The phantom simulates not only the morphologic characteristics of biological tissue but also absorption and scattering properties. The printing system is based on a 3D motion platform with coordinated control of the DC motors. A special jet nozzle is designed to mix base, scattering, and absorption materials at different ratios. 3D tissue structures are fabricated through layer-by-layer printing with selective deposition of phantom materials of different ingredients. Different mixed ratios of base, scattering and absorption materials have been tested in order to optimize the printing outcome. A spectrometer and a tissue spectrophotometer are used for characterizing phantom absorption and scattering properties. The goal of this project is to fabricate skin tissue simulating phantoms as a traceable standard for the calibration of biomedical optical spectral devices.

  7. Design of 3D scanner for surface contour mapping by ultrasonic sensor

    NASA Astrophysics Data System (ADS)

    Munir, Muhammad Miftahul; Billah, Mohammad Aziz; Surachman, Arif; Budiman, Maman; Khairurrijal

    2015-04-01

    Surface mapping systems have attracted great attention due to their potential applications in many areas. In this paper, a simple 3D scanner based on ultrasonic sensor was designed for mapping a contour of object surface. The scanner using an SRF02 ultrasonic sensor, a microcontroller and radio frequency (RF) module to collect coordinates of object surface (point cloud), and sent data to computer. The point cloud collection process was performed by moving two ultrasonic sensors in y and x directions. Both sensors measure a distance from an object surface to a reference point of each sensor. The measurement results represent the point cloud of object surface and the data will be sent to computer via RF module. The point cloud then converted to 3D model using MATLAB. It was found that the object contours can be reconstructed very well by the developed 3D scanner system.

  8. Fish body surface data measurement based on 3D digital image correlation

    NASA Astrophysics Data System (ADS)

    Jiang, Ming; Qian, Chen; Yang, Wenkai

    2016-01-01

    To film the moving fish in the glass tank, light will be bent at the interface of air and glass, glass and water. Based on binocular stereo vision and refraction principle, we establish a mathematical model of 3D image correlation to reconstruct the 3D coordinates of samples in the water. Marking speckle in fish surface, a series of real-time speckle images of swimming fish will be obtained by two high-speed cameras, and instantaneous 3D shape, strain, displacement etc. of fish will be reconstructed.

  9. Parallel robot for micro assembly with integrated innovative optical 3D-sensor

    NASA Astrophysics Data System (ADS)

    Hesselbach, Juergen; Ispas, Diana; Pokar, Gero; Soetebier, Sven; Tutsch, Rainer

    2002-10-01

    Recent advances in the fields of MEMS and MOEMS often require precise assembly of very small parts with an accuracy of a few microns. In order to meet this demand, a new approach using a robot based on parallel mechanisms in combination with a novel 3D-vision system has been chosen. The planar parallel robot structure with 2 DOF provides a high resolution in the XY-plane. It carries two additional serial axes for linear and rotational movement in/about z direction. In order to achieve high precision as well as good dynamic capabilities, the drive concept for the parallel (main) axes incorporates air bearings in combination with a linear electric servo motors. High accuracy position feedback is provided by optical encoders with a resolution of 0.1 μm. To allow for visualization and visual control of assembly processes, a camera module fits into the hollow tool head. It consists of a miniature CCD camera and a light source. In addition a modular gripper support is integrated into the tool head. To increase the accuracy a control loop based on an optoelectronic sensor will be implemented. As a result of an in-depth analysis of different approaches a photogrammetric system using one single camera and special beam-splitting optics was chosen. A pattern of elliptical marks is applied to the surfaces of workpiece and gripper. Using a model-based recognition algorithm the image processing software identifies the gripper and the workpiece and determines their relative position. A deviation vector is calculated and fed into the robot control to guide the gripper.

  10. Macro optical projection tomography for large scale 3D imaging of plant structures and gene activity.

    PubMed

    Lee, Karen J I; Calder, Grant M; Hindle, Christopher R; Newman, Jacob L; Robinson, Simon N; Avondo, Jerome J H Y; Coen, Enrico S

    2016-12-26

    Optical projection tomography (OPT) is a well-established method for visualising gene activity in plants and animals. However, a limitation of conventional OPT is that the specimen upper size limit precludes its application to larger structures. To address this problem we constructed a macro version called Macro OPT (M-OPT). We apply M-OPT to 3D live imaging of gene activity in growing whole plants and to visualise structural morphology in large optically cleared plant and insect specimens up to 60 mm tall and 45 mm deep. We also show how M-OPT can be used to image gene expression domains in 3D within fixed tissue and to visualise gene activity in 3D in clones of growing young whole Arabidopsis plants. A further application of M-OPT is to visualise plant-insect interactions. Thus M-OPT provides an effective 3D imaging platform that allows the study of gene activity, internal plant structures and plant-insect interactions at a macroscopic scale.

  11. Design and verification of diffractive optical elements for speckle generation of 3-D range sensors

    NASA Astrophysics Data System (ADS)

    Du, Pei-Qin; Shih, Hsi-Fu; Chen, Jenq-Shyong; Wang, Yi-Shiang

    2016-12-01

    The optical projection using speckles is one of the structured light methods that have been applied to three-dimensional (3-D) range sensors. This paper investigates the design and fabrication of diffractive optical elements (DOEs) for generating the light field with uniformly distributed speckles. Based on the principles of computer generated holograms, the iterative Fourier transform algorithm was adopted for the DOE design. It was used to calculate the phase map for diffracting the incident laser beam into a goal pattern with distributed speckles. Four patterns were designed in the study. Their phase maps were first examined by a spatial light modulator and then fabricated on glass substrates by microfabrication processes. Finally, the diffraction characteristics of the fabricated devices were verified. The experimental results show that the proposed methods are applicable to the DOE design of 3-D range sensors. Furthermore, any expected diffraction area and speckle density could be possibly achieved according to the relations presented in the paper.

  12. Parametric estimation of 3D tubular structures for diffuse optical tomography

    PubMed Central

    Larusson, Fridrik; Anderson, Pamela G.; Rosenberg, Elizabeth; Kilmer, Misha E.; Sassaroli, Angelo; Fantini, Sergio; Miller, Eric L.

    2013-01-01

    We explore the use of diffuse optical tomography (DOT) for the recovery of 3D tubular shapes representing vascular structures in breast tissue. Using a parametric level set method (PaLS) our method incorporates the connectedness of vascular structures in breast tissue to reconstruct shape and absorption values from severely limited data sets. The approach is based on a decomposition of the unknown structure into a series of two dimensional slices. Using a simplified physical model that ignores 3D effects of the complete structure, we develop a novel inter-slice regularization strategy to obtain global regularity. We report on simulated and experimental reconstructions using realistic optical contrasts where our method provides a more accurate estimate compared to an unregularized approach and a pixel based reconstruction. PMID:23411913

  13. Parametric estimation of 3D tubular structures for diffuse optical tomography.

    PubMed

    Larusson, Fridrik; Anderson, Pamela G; Rosenberg, Elizabeth; Kilmer, Misha E; Sassaroli, Angelo; Fantini, Sergio; Miller, Eric L

    2013-02-01

    We explore the use of diffuse optical tomography (DOT) for the recovery of 3D tubular shapes representing vascular structures in breast tissue. Using a parametric level set method (PaLS) our method incorporates the connectedness of vascular structures in breast tissue to reconstruct shape and absorption values from severely limited data sets. The approach is based on a decomposition of the unknown structure into a series of two dimensional slices. Using a simplified physical model that ignores 3D effects of the complete structure, we develop a novel inter-slice regularization strategy to obtain global regularity. We report on simulated and experimental reconstructions using realistic optical contrasts where our method provides a more accurate estimate compared to an unregularized approach and a pixel based reconstruction.

  14. Constructing topologically connected surfaces for the comprehensive analysis of 3-D medical structures

    NASA Astrophysics Data System (ADS)

    Kalvin, Alan D.; Cutting, Court B.; Haddad, Betsy; Noz, Marilyn E.

    1991-06-01

    Three-dimensional (3D) medical imaging deals with the visualization, manipulation, and measuring of objects in 3D medical images. So far, research efforts have concentrated primarily on visualization, using well-developed methods from computer graphics. Very little has been achieved in developing techniques for manipulating medical objects, or for extracting quantitative measurements from them beyond volume calculation (by counting voxels), and computing distances and angles between manually located surface points. A major reason for the slow pace in the development of manipulation and quantification methods lies with the limitations of current algorithms for constructing surfaces from 3D solid objects. We show that current surface construction algorithms either (a) do not construct valid surface descriptions of solid objects or (b) produce surface representations that are not particularly suitable for anything other than visualization. We present ALLIGATOR, a new surface construction algorithm that produces valid, topologically connected surface representations of solid objects. We have developed a modeling system based on the surface representations created by ALLIGATOR that is suitable for developing algorithms to visualize, manipulate, and quantify 3D medical objects. Using this modeling system we have developed a method for efficiently computing principle curvatures and directions on surfaces. These measurements form the basis for a new metric system being developed for morphometrics. The modeling system is also being used in the development of systems for quantitative pre-surgical planning and surgical augmentation.

  15. Representation of 3-D surface orientation by velocity and disparity gradient cues in area MT.

    PubMed

    Sanada, Takahisa M; Nguyenkim, Jerry D; Deangelis, Gregory C

    2012-04-01

    Neural coding of the three-dimensional (3-D) orientation of planar surface patches may be an important intermediate step in constructing representations of complex 3-D surface structure. Spatial gradients of binocular disparity, image velocity, and texture provide potent cues to the 3-D orientation (tilt and slant) of planar surfaces. Previous studies have described neurons in both dorsal and ventral stream areas that are selective for surface tilt based on one or more of these gradient cues. However, relatively little is known about whether single neurons provide consistent information about surface orientation from multiple gradient cues. Moreover, it is unclear how neural responses to combinations of surface orientation cues are related to responses to the individual cues. We measured responses of middle temporal (MT) neurons to random dot stimuli that simulated planar surfaces at a variety of tilts and slants. Four cue conditions were tested: disparity, velocity, and texture gradients alone, as well as all three gradient cues combined. Many neurons showed robust tuning for surface tilt based on disparity and velocity gradients, with relatively little selectivity for texture gradients. Some neurons showed consistent tilt preferences for disparity and velocity cues, whereas others showed large discrepancies. Responses to the combined stimulus were generally well described as a weighted linear sum of responses to the individual cues, even when disparity and velocity preferences were discrepant. These findings suggest that area MT contains a rudimentary representation of 3-D surface orientation based on multiple cues, with single neurons implementing a simple cue integration rule.

  16. 3D surface reconstruction based on image stitching from gastric endoscopic video sequence

    NASA Astrophysics Data System (ADS)

    Duan, Mengyao; Xu, Rong; Ohya, Jun

    2013-09-01

    This paper proposes a method for reconstructing 3D detailed structures of internal organs such as gastric wall from endoscopic video sequences. The proposed method consists of the four major steps: Feature-point-based 3D reconstruction, 3D point cloud stitching, dense point cloud creation and Poisson surface reconstruction. Before the first step, we partition one video sequence into groups, where each group consists of two successive frames (image pairs), and each pair in each group contains one overlapping part, which is used as a stitching region. Fist, the 3D point cloud of each group is reconstructed by utilizing structure from motion (SFM). Secondly, a scheme based on SIFT features registers and stitches the obtained 3D point clouds, by estimating the transformation matrix of the overlapping part between different groups with high accuracy and efficiency. Thirdly, we select the most robust SIFT feature points as the seed points, and then obtain the dense point cloud from sparse point cloud via a depth testing method presented by Furukawa. Finally, by utilizing Poisson surface reconstruction, polygonal patches for the internal organs are obtained. Experimental results demonstrate that the proposed method achieves a high accuracy and efficiency for 3D reconstruction of gastric surface from an endoscopic video sequence.

  17. A photogrammetry-based system for 3D surface reconstruction of prosthetics and orthotics.

    PubMed

    Li, Guang-kun; Gao, Fan; Wang, Zhi-gang

    2011-01-01

    The objective of this study is to develop an innovative close range digital photogrammetry (CRDP) system using the commercial digital SLR cameras to measure and reconstruct the 3D surface of prosthetics and orthotics. This paper describes the instrumentation, techniques and preliminary results of the proposed system. The technique works by taking pictures of the object from multiple view angles. The series of pictures were post-processed via feature point extraction, point match and 3D surface reconstruction. In comparison with the traditional method such as laser scanning, the major advantages of our instrument include the lower cost, compact and easy-to-use hardware, satisfactory measurement accuracy, and significantly less measurement time. Besides its potential applications in prosthetics and orthotics surface measurement, the simple setup and its ease of use will make it suitable for various 3D surface reconstructions.

  18. Research in Image Understanding as Applied to 3-D Microwave Tomographic Imaging with Near Optical Resolution.

    DTIC Science & Technology

    1986-03-10

    Severe Clutter .... ........ 1I-i III . Optical Implementation of the HopfieldModel .I -? .- . ." Model........................ . . BY...can be employed in future broad-band imaging radar networks capable of providing 3-D projective or . - tomographic images of remote aerospace targets...We expect the results of this effort to tell us how to achieve centimeter resolution on remote aerospace objects cost-effectively using microwave

  19. Performance of a commercial optical CT scanner and polymer gel dosimeters for 3-D dose verification.

    PubMed

    Xu, Y; Wuu, Cheng-Shie; Maryanski, Marek J

    2004-11-01

    Performance analysis of a commercial three-dimensional (3-D) dose mapping system based on optical CT scanning of polymer gels is presented. The system consists of BANG 3 polymer gels (MGS Research, Inc., Madison, CT), OCTOPUS laser CT scanner (MGS Research, Inc., Madison, CT), and an in-house developed software for optical CT image reconstruction and 3-D dose distribution comparison between the gel, film measurements and the radiation therapy treatment plans. Various sources of image noise (digitization, electronic, optical, and mechanical) generated by the scanner as well as optical uniformity of the polymer gel are analyzed. The performance of the scanner is further evaluated in terms of the reproducibility of the data acquisition process, the uncertainties at different levels of reconstructed optical density per unit length and the effects of scanning parameters. It is demonstrated that for BANG 3 gel phantoms held in cylindrical plastic containers, the relative dose distribution can be reproduced by the scanner with an overall uncertainty of about 3% within approximately 75% of the radius of the container. In regions located closer to the container wall, however, the scanner generates erroneous optical density values that arise from the reflection and refraction of the laser rays at the interface between the gel and the container. The analysis of the accuracy of the polymer gel dosimeter is exemplified by the comparison of the gel/OCT-derived dose distributions with those from film measurements and a commercial treatment planning system (Cadplan, Varian Corporation, Palo Alto, CA) for a 6 cm x 6 cm single field of 6 MV x rays and a 3-D conformal radiotherapy (3DCRT) plan. The gel measurements agree with the treatment plans and the film measurements within the "3%-or-2 mm" criterion throughout the usable, artifact-free central region of the gel volume. Discrepancies among the three data sets are analyzed.

  20. 3-D Surface Depression Profiling Using High Frequency Focused Air-Coupled Ultrasonic Pulses

    NASA Technical Reports Server (NTRS)

    Roth, Don J.; Kautz, Harold E.; Abel, Phillip B.; Whalen, Mike F.; Hendricks, J. Lynne; Bodis, James R.

    1999-01-01

    Surface topography is an important variable in the performance of many industrial components and is normally measured with diamond-tip profilometry over a small area or using optical scattering methods for larger area measurement. This article shows quantitative surface topography profiles as obtained using only high-frequency focused air-coupled ultrasonic pulses. The profiles were obtained using a profiling system developed by NASA Glenn Research Center and Sonix, Inc (via a formal cooperative agreement). (The air transducers are available as off-the-shelf items from several companies.) The method is simple and reproducible because it relies mainly on knowledge and constancy of the sound velocity through the air. The air transducer is scanned across the surface and sends pulses to the sample surface where they are reflected back from the surface along the same path as the incident wave. Time-of-flight images of the sample surface are acquired and converted to depth/surface profile images using the simple relation (d = V*t/2) between distance (d), time-of-flight (t), and the velocity of sound in air (V). The system has the ability to resolve surface depression variations as small as 25 microns, is useable over a 1.4 mm vertical depth range, and can profile large areas only limited by the scan limits of the particular ultrasonic system. (Best-case depth resolution is 0.25 microns which may be achievable with improved isolation from vibration and air currents.) The method using an optimized configuration is reasonably rapid and has all quantitative analysis facilities on-line including 2-D and 3-D visualization capability, extreme value filtering (for faulty data), and leveling capability. Air-coupled surface profilometry is applicable to plate-like and curved samples. In this article, results are shown for several proof-of-concept samples, plastic samples burned in microgravity on the STS-54 space shuttle mission, and a partially-coated cylindrical ceramic

  1. Fluorescent stereo microscopy for 3D surface profilometry and deformation mapping.

    PubMed

    Hu, Zhenxing; Luo, Huiyang; Du, Yingjie; Lu, Hongbing

    2013-05-20

    Recently, mechanobiology has received increased attention. For investigation of biofilm and cellular tissue, measurements of the surface topography and deformation in real-time are a pre-requisite for understanding the growth mechanisms. In this paper, a novel three-dimensional (3D) fluorescent microscopic method for surface profilometry and deformation measurements is developed. In this technique a pair of cameras are connected to a binocular fluorescent microscope to acquire micrographs from two different viewing angles of a sample surface doped or sprayed with fluorescent microparticles. Digital image correlation technique is used to search for matching points in the pairing fluorescence micrographs. After calibration of the system, the 3D surface topography is reconstructed from the pair of planar images. When the deformed surface topography is compared with undeformed topography using fluorescent microparticles for movement tracking of individual material points, the full field deformation of the surface is determined. The technique is demonstrated on topography measurement of a biofilm, and also on surface deformation measurement of the biofilm during growth. The use of 3D imaging of the fluorescent microparticles eliminates the formation of bright parts in an image caused by specular reflections. The technique is appropriate for non-contact, full-field and real-time 3D surface profilometry and deformation measurements of materials and structures at the microscale.

  2. 3-D Acoustic Scattering from 2-D Rough Surfaces Using A Parabolic Equation Model

    DTIC Science & Technology

    2013-12-01

    acoustic propagation signals, especially at mid- frequencies and higher (e.g., acoustic communications systems). For many years, the effects of rough...of the effect of surface scattering on 3-D propagation , which is critical in evaluating the variability in underwater acoustic propagation . Results...14. SUBJECT TERMS Acoustic Propagation , Acoustic Scattering, Sea Surface Perturbations, Split- Step Fourier Algorithm, Finite Difference Algorithm

  3. Characterization of a parallel beam CCD optical-CT apparatus for 3D radiation dosimetry

    NASA Astrophysics Data System (ADS)

    Krstajić, Nikola; Doran, Simon J.

    2006-12-01

    This paper describes the initial steps we have taken in establishing CCD based optical-CT as a viable alternative for 3-D radiation dosimetry. First, we compare the optical density (OD) measurements from a high quality test target and variable neutral density filter (VNDF). A modulation transfer function (MTF) of individual projections is derived for three positions of the sinusoidal test target within the scanning tank. Our CCD is then characterized in terms of its signal-to-noise ratio (SNR). Finally, a sample reconstruction of a scan of a PRESAGETM (registered trademark of Heuris Pharma, NJ, Skillman, USA.) dosimeter is given, demonstrating the capabilities of the apparatus.

  4. A continuous surface reconstruction method on point cloud captured from a 3D surface photogrammetry system

    SciTech Connect

    Liu, Wenyang; Cheung, Yam; Sabouri, Pouya; Arai, Tatsuya J.; Sawant, Amit; Ruan, Dan

    2015-11-15

    achieved submillimeter reconstruction RMSE under different configurations, demonstrating quantitatively the faith of the proposed method in preserving local structural properties of the underlying surface in the presence of noise and missing measurements, and its robustness toward variations of such characteristics. On point clouds from the human subject, the proposed method successfully reconstructed all patient surfaces, filling regions where raw point coordinate readings were missing. Within two comparable regions of interest in the chest area, similar mean curvature distributions were acquired from both their reconstructed surface and CT surface, with mean and standard deviation of (μ{sub recon} = − 2.7 × 10{sup −3} mm{sup −1}, σ{sub recon} = 7.0 × 10{sup −3} mm{sup −1}) and (μ{sub CT} = − 2.5 × 10{sup −3} mm{sup −1}, σ{sub CT} = 5.3 × 10{sup −3} mm{sup −1}), respectively. The agreement of local geometry properties between the reconstructed surfaces and the CT surface demonstrated the ability of the proposed method in faithfully representing the underlying patient surface. Conclusions: The authors have integrated and developed an accurate level-set based continuous surface reconstruction method on point clouds acquired by a 3D surface photogrammetry system. The proposed method has generated a continuous representation of the underlying phantom and patient surfaces with good robustness against noise and missing measurements. It serves as an important first step for further development of motion tracking methods during radiotherapy.

  5. A continuous surface reconstruction method on point cloud captured from a 3D surface photogrammetry system

    PubMed Central

    Liu, Wenyang; Cheung, Yam; Sabouri, Pouya; Arai, Tatsuya J.; Sawant, Amit; Ruan, Dan

    2015-01-01

    achieved submillimeter reconstruction RMSE under different configurations, demonstrating quantitatively the faith of the proposed method in preserving local structural properties of the underlying surface in the presence of noise and missing measurements, and its robustness toward variations of such characteristics. On point clouds from the human subject, the proposed method successfully reconstructed all patient surfaces, filling regions where raw point coordinate readings were missing. Within two comparable regions of interest in the chest area, similar mean curvature distributions were acquired from both their reconstructed surface and CT surface, with mean and standard deviation of (μrecon = − 2.7 × 10−3 mm−1, σrecon = 7.0 × 10−3 mm−1) and (μCT = − 2.5 × 10−3 mm−1, σCT = 5.3 × 10−3 mm−1), respectively. The agreement of local geometry properties between the reconstructed surfaces and the CT surface demonstrated the ability of the proposed method in faithfully representing the underlying patient surface. Conclusions: The authors have integrated and developed an accurate level-set based continuous surface reconstruction method on point clouds acquired by a 3D surface photogrammetry system. The proposed method has generated a continuous representation of the underlying phantom and patient surfaces with good robustness against noise and missing measurements. It serves as an important first step for further development of motion tracking methods during radiotherapy. PMID:26520747

  6. First steps toward 3D high resolution imaging using adaptive optics and full-field optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Blanco, Leonardo; Blavier, Marie; Glanc, Marie; Pouplard, Florence; Tick, Sarah; Maksimovic, Ivan; Chenegros, Guillaume; Mugnier, Laurent; Lacombe, Francois; Rousset, Gérard; Paques, Michel; Le Gargasson, Jean-François; Sahel, Jose-Alain

    2008-09-01

    We describe here two parts of our future 3D fundus camera coupling Adaptive Optics and full-field Optical Coherence Tomography. The first part is an Adaptive Optics flood imager installed at the Quinze-Vingts Hospital, regularly used on healthy and pathological eyes. A posteriori image reconstruction is performed, increasing the final image quality and field of view. The instrument lateral resolution is better than 2 microns. The second part is a full-field Optical Coherence Tomograph, which has demonstrated capability of performing a simple kind of "4 phases" image reconstruction of non biological samples and ex situ retinas. Final aim is to couple both parts in order to achieve 3D high resolution mapping of in vivo retinas.

  7. 3D surface measurements with isogeometric stereocorrelation-Application to complex shapes

    NASA Astrophysics Data System (ADS)

    Dufour, John-Eric; Leclercq, Sylvain; Schneider, Julien; Roux, Stéphane; Hild, François

    2016-12-01

    The aim of the present study is to measure complex shapes of tested objects by using a priori information given by their CAD model via stereocorrelation. To follow a 3D object during its deformation and to determine 3D surface displacement fields, a first measurement of the object shape is necessary. It is achieved by updating the CAD reference via a global approach to stereocorrelation. Once the 3D shape has been determined, the next step is to measure 3D displacement fields during loading. The kinematics of the deformed shape is assumed to be written within the same isogeometric framework. Isogeometric stereocorrelation is applied to analyze a compression test on a ribbed cylinder in two different configurations of the stereo rig.

  8. 3D numerical simulation analysis of passive drag near free surface in swimming

    NASA Astrophysics Data System (ADS)

    Zhan, Jie-min; Li, Tian-zeng; Chen, Xue-bin; Li, Yok-sheung; Wai, Wing-hong Onyx

    2015-04-01

    The aim of this work is to build a 3D numerical model to study the characteristics of passive drag on competitive swimmers taking into account the impact of the free surface. This model solves the 3D incompressible Navier-Stokes equations using RNG k- ɛ turbulence closure. The volume of fluid (VOF) method is used to locate the free surface. The 3D virtual model is created by Computer Aided Industrial Design (CAID) software, Rhinoceros. Firstly, a specific posture of swimming is studied. The simulation results are in good agreement with the data from mannequin towing experiments. The effects of a swimmer's arms and legs positions on swimming performance are then studied. Finally, it is demonstrated that the present method is capable of simulating gliding near the free surface.

  9. Numerical Investigation of 3D multichannel analysis of surface wave method

    NASA Astrophysics Data System (ADS)

    Wang, Limin; Xu, Yixian; Luo, Yinhe

    2015-08-01

    Multichannel analysis of surface wave (MASW) method is an efficient tool to obtain near-surface S-wave velocity, and it has gained popularity in engineering practice. Up to now, most examples of using the MASW technique are focused on 2D models or data from a 1D linear receiver spread. We propose a 3D MASW scheme. A finite-difference (FD) method is used to investigate the method using linear and fan-shaped receiver spreads. Results show that the 3D topography strongly affects propagation of Rayleigh waves. The energy concentration of dispersion image is distorted and bifurcated because of the influence of free-surface topography. These effects are reduced with the 3D MASW method. Lastly we investigate the relation between the array size and the resolution of dispersion measurement.

  10. A joint estimation detection of Glaucoma progression in 3D spectral domain optical coherence tomography optic nerve head images

    PubMed Central

    Belghith, Akram; Bowd, Christopher; Weinreb, Robert N.; Zangwill, Linda M.

    2014-01-01

    Glaucoma is an ocular disease characterized by distinctive changes in the optic nerve head (ONH) and visual field. Glaucoma can strike without symptoms and causes blindness if it remains without treatment. Therefore, early disease detection is important so that treatment can be initiated and blindness prevented. In this context, important advances in technology for non-invasive imaging of the eye have been made providing quantitative tools to measure structural changes in ONH topography, an essential element for glaucoma detection and monitoring. 3D spectral domain optical coherence tomography (SD-OCT), an optical imaging technique, has been commonly used to discriminate glaucomatous from healthy subjects. In this paper, we present a new framework for detection of glaucoma progression using 3D SD-OCT images. In contrast to previous works that the retinal nerve fiber layer (RNFL) thickness measurement provided by commercially available spectral-domain optical coherence tomograph, we consider the whole 3D volume for change detection. To integrate a priori knowledge and in particular the spatial voxel dependency in the change detection map, we propose the use of the Markov Random Field to handle a such dependency. To accommodate the presence of false positive detection, the estimated change detection map is then used to classify a 3D SDOCT image into the “non-progressing” and “progressing” glaucoma classes, based on a fuzzy logic classifier. We compared the diagnostic performance of the proposed framework to existing methods of progression detection. PMID:25606299

  11. A joint estimation detection of Glaucoma progression in 3D spectral domain optical coherence tomography optic nerve head images

    NASA Astrophysics Data System (ADS)

    Belghith, Akram; Bowd, Christopher; Weinreb, Robert N.; Zangwill, Linda M.

    2014-03-01

    Glaucoma is an ocular disease characterized by distinctive changes in the optic nerve head (ONH) and visual field. Glaucoma can strike without symptoms and causes blindness if it remains without treatment. Therefore, early disease detection is important so that treatment can be initiated and blindness prevented. In this context, important advances in technology for non-invasive imaging of the eye have been made providing quantitative tools to measure structural changes in ONH topography, an essential element for glaucoma detection and monitoring. 3D spectral domain optical coherence tomography (SD-OCT), an optical imaging technique, has been commonly used to discriminate glaucomatous from healthy subjects. In this paper, we present a new framework for detection of glaucoma progression using 3D SD-OCT images. In contrast to previous works that the retinal nerve fiber layer (RNFL) thickness measurement provided by commercially available spectral-domain optical coherence tomograph, we consider the whole 3D volume for change detection. To integrate a priori knowledge and in particular the spatial voxel dependency in the change detection map, we propose the use of the Markov Random Field to handle a such dependency. To accommodate the presence of false positive detection, the estimated change detection map is then used to classify a 3D SDOCT image into the "non-progressing" and "progressing" glaucoma classes, based on a fuzzy logic classifier. We compared the diagnostic performance of the proposed framework to existing methods of progression detection.

  12. A comprehensive evaluation of the PRESAGE/optical-CT 3D dosimetry system

    SciTech Connect

    Sakhalkar, H. S.; Adamovics, J.; Ibbott, G.; Oldham, M.

    2009-01-15

    This work presents extensive investigations to evaluate the robustness (intradosimeter consistency and temporal stability of response), reproducibility, precision, and accuracy of a relatively new 3D dosimetry system comprising a leuco-dye doped plastic 3D dosimeter (PRESAGE) and a commercial optical-CT scanner (OCTOPUS 5x scanner from MGS Research, Inc). Four identical PRESAGE 3D dosimeters were created such that they were compatible with the Radiologic Physics Center (RPC) head-and-neck (H and N) IMRT credentialing phantom. Each dosimeter was irradiated with a rotationally symmetric arrangement of nine identical small fields (1x3 cm{sup 2}) impinging on the flat circular face of the dosimeter. A repetitious sequence of three dose levels (4, 2.88, and 1.28 Gy) was delivered. The rotationally symmetric treatment resulted in a dose distribution with high spatial variation in axial planes but only gradual variation with depth along the long axis of the dosimeter. The significance of this treatment was that it facilitated accurate film dosimetry in the axial plane, for independent verification. Also, it enabled rigorous evaluation of robustness, reproducibility and accuracy of response, at the three dose levels. The OCTOPUS 5x commercial scanner was used for dose readout from the dosimeters at daily time intervals. The use of improved optics and acquisition technique yielded substantially improved noise characteristics (reduced to {approx}2%) than has been achieved previously. Intradosimeter uniformity of radiochromic response was evaluated by calculating a 3D gamma comparison between each dosimeter and axially rotated copies of the same dosimeter. This convenient technique exploits the rotational symmetry of the distribution. All points in the gamma comparison passed a 2% difference, 1 mm distance-to-agreement criteria indicating excellent intradosimeter uniformity even at low dose levels. Postirradiation, the dosimeters were all found to exhibit a slight increase in

  13. A comprehensive evaluation of the PRESAGE/optical-CT 3D dosimetry system.

    PubMed

    Sakhalkar, H S; Adamovics, J; Ibbott, G; Oldham, M

    2009-01-01

    This work presents extensive investigations to evaluate the robustness (intradosimeter consistency and temporal stability of response), reproducibility, precision, and accuracy of a relatively new 3D dosimetry system comprising a leuco-dye doped plastic 3D dosimeter (PRESAGE) and a commercial optical-CT scanner (OCTOPUS 5x scanner from MGS Research, Inc). Four identical PRESAGE 3D dosimeters were created such that they were compatible with the Radiologic Physics Center (RPC) head-and-neck (H&N) IMRT credentialing phantom. Each dosimeter was irradiated with a rotationally symmetric arrangement of nine identical small fields (1 x 3 cm2) impinging on the flat circular face of the dosimeter. A repetitious sequence of three dose levels (4, 2.88, and 1.28 Gy) was delivered. The rotationally symmetric treatment resulted in a dose distribution with high spatial variation in axial planes but only gradual variation with depth along the long axis of the dosimeter. The significance of this treatment was that it facilitated accurate film dosimetry in the axial plane, for independent verification. Also, it enabled rigorous evaluation of robustness, reproducibility and accuracy of response, at the three dose levels. The OCTOPUS 5x commercial scanner was used for dose readout from the dosimeters at daily time intervals. The use of improved optics and acquisition technique yielded substantially improved noise characteristics (reduced to approximately 2%) than has been achieved previously. Intradosimeter uniformity of radiochromic response was evaluated by calculating a 3D gamma comparison between each dosimeter and axially rotated copies of the same dosimeter. This convenient technique exploits the rotational symmetry of the distribution. All points in the gamma comparison passed a 2% difference, 1 mm distance-to-agreement criteria indicating excellent intradosimeter uniformity even at low dose levels. Postirradiation, the dosimeters were all found to exhibit a slight increase in

  14. Structural and functional imaging of 3D microfluidic mixers using optical coherence tomography.

    PubMed

    Xi, Chuanwu; Marks, Daniel L; Parikh, Devang S; Raskin, Lutgarde; Boppart, Stephen A

    2004-05-18

    To achieve high mixing efficiency in microfluidic devices, complex designs are often required. Microfluidic devices have been evaluated with light and confocal microscopy, but fluid-flow characteristics at different depths are difficult to separate from the en face images produced. By using optical coherence tomography (OCT), an imaging modality capable of imaging 3D microstructures at micrometer-scale resolutions over millimeter-size scales, we obtained 3D dynamic functional and structural data for three representative microfluidic mixers: a Y channel mixer, a 3D serpentine mixer, and a vortex mixer. In the serpentine mixer, OCT image analysis revealed that the mixing efficiency was linearly dependent on the Reynolds number, whereas it appeared to have exponential dependence when imaged with light microscopy. The visual overlap of fluid flows in light-microscopy images leads to an overestimation of the mixing efficiency, an effect that was eliminated with OCT imaging. Doppler OCT measurements determined velocity profiles at various points in the serpentine mixer. Mixing patterns in the vortex mixer were compared with light-microscopy and OCT image analysis. These results demonstrate that OCT can significantly improve the characterization of 3D microfluidic device structure and function.

  15. Multiview 3-D Echocardiography Fusion with Breath-Hold Position Tracking Using an Optical Tracking System.

    PubMed

    Punithakumar, Kumaradevan; Hareendranathan, Abhilash R; McNulty, Alexander; Biamonte, Marina; He, Allen; Noga, Michelle; Boulanger, Pierre; Becher, Harald

    2016-08-01

    Recent advances in echocardiography allow real-time 3-D dynamic image acquisition of the heart. However, one of the major limitations of 3-D echocardiography is the limited field of view, which results in an acquisition insufficient to cover the whole geometry of the heart. This study proposes the novel approach of fusing multiple 3-D echocardiography images using an optical tracking system that incorporates breath-hold position tracking to infer that the heart remains at the same position during different acquisitions. In six healthy male volunteers, 18 pairs of apical/parasternal 3-D ultrasound data sets were acquired during a single breath-hold as well as in subsequent breath-holds. The proposed method yielded a field of view improvement of 35.4 ± 12.5%. To improve the quality of the fused image, a wavelet-based fusion algorithm was developed that computes pixelwise likelihood values for overlapping voxels from multiple image views. The proposed wavelet-based fusion approach yielded significant improvement in contrast (66.46 ± 21.68%), contrast-to-noise ratio (49.92 ± 28.71%), signal-to-noise ratio (57.59 ± 47.85%) and feature count (13.06 ± 7.44%) in comparison to individual views.

  16. surf3d: A 3-D finite-element program for the analysis of surface and corner cracks in solids subjected to mode-1 loadings

    NASA Technical Reports Server (NTRS)

    Raju, I. S.; Newman, J. C., Jr.

    1993-01-01

    A computer program, surf3d, that uses the 3D finite-element method to calculate the stress-intensity factors for surface, corner, and embedded cracks in finite-thickness plates with and without circular holes, was developed. The cracks are assumed to be either elliptic or part eliptic in shape. The computer program uses eight-noded hexahedral elements to model the solid. The program uses a skyline storage and solver. The stress-intensity factors are evaluated using the force method, the crack-opening displacement method, and the 3-D virtual crack closure methods. In the manual the input to and the output of the surf3d program are described. This manual also demonstrates the use of the program and describes the calculation of the stress-intensity factors. Several examples with sample data files are included with the manual. To facilitate modeling of the user's crack configuration and loading, a companion program (a preprocessor program) that generates the data for the surf3d called gensurf was also developed. The gensurf program is a three dimensional mesh generator program that requires minimal input and that builds a complete data file for surf3d. The program surf3d is operational on Unix machines such as CRAY Y-MP, CRAY-2, and Convex C-220.

  17. Cold atmospheric plasma (CAP) surface nanomodified 3D printed polylactic acid (PLA) scaffolds for bone regeneration.

    PubMed

    Wang, Mian; Favi, Pelagie; Cheng, Xiaoqian; Golshan, Negar H; Ziemer, Katherine S; Keidar, Michael; Webster, Thomas J

    2016-12-01

    Three-dimensional (3D) printing is a new fabrication method for tissue engineering which can precisely control scaffold architecture at the micron-scale. However, scaffolds not only need 3D biocompatible structures that mimic the micron structure of natural tissues, they also require mimicking of the nano-scale extracellular matrix properties of the tissue they intend to replace. In order to achieve this, the objective of the present in vitro study was to use cold atmospheric plasma (CAP) as a quick and inexpensive way to modify the nano-scale roughness and chemical composition of a 3D printed scaffold surface. Water contact angles of a normal 3D printed poly-lactic-acid (PLA) scaffold dramatically dropped after CAP treatment from 70±2° to 24±2°. In addition, the nano-scale surface roughness (Rq) of the untreated 3D PLA scaffolds drastically increased (up to 250%) after 1, 3, and 5min of CAP treatment from 1.20nm to 10.50nm, 22.90nm, and 27.60nm, respectively. X-ray photoelectron spectroscopy (XPS) analysis showed that the ratio of oxygen to carbon significantly increased after CAP treatment, which indicated that the CAP treatment of PLA not only changed nano-scale roughness but also chemistry. Both changes in hydrophilicity and nano-scale roughness demonstrated a very efficient plasma treatment, which in turn significantly promoted both osteoblast (bone forming cells) and mesenchymal stem cell attachment and proliferation. These promising results suggest that CAP surface modification may have potential applications for enhancing 3D printed PLA bone tissue engineering materials (and all 3D printed materials) in a quick and an inexpensive manner and, thus, should be further studied.

  18. Automated 3D Damaged Cavity Model Builder for Lower Surface Acreage Tile on Orbiter

    NASA Technical Reports Server (NTRS)

    Belknap, Shannon; Zhang, Michael

    2013-01-01

    The 3D Automated Thermal Tool for Damaged Acreage Tile Math Model builder was developed to perform quickly and accurately 3D thermal analyses on damaged lower surface acreage tiles and structures beneath the damaged locations on a Space Shuttle Orbiter. The 3D model builder created both TRASYS geometric math models (GMMs) and SINDA thermal math models (TMMs) to simulate an idealized damaged cavity in the damaged tile(s). The GMMs are processed in TRASYS to generate radiation conductors between the surfaces in the cavity. The radiation conductors are inserted into the TMMs, which are processed in SINDA to generate temperature histories for all of the nodes on each layer of the TMM. The invention allows a thermal analyst to create quickly and accurately a 3D model of a damaged lower surface tile on the orbiter. The 3D model builder can generate a GMM and the correspond ing TMM in one or two minutes, with the damaged cavity included in the tile material. A separate program creates a configuration file, which would take a couple of minutes to edit. This configuration file is read by the model builder program to determine the location of the damage, the correct tile type, tile thickness, structure thickness, and SIP thickness of the damage, so that the model builder program can build an accurate model at the specified location. Once the models are built, they are processed by the TRASYS and SINDA.

  19. 3D surface reconstruction of apples from 2D NIR images

    NASA Astrophysics Data System (ADS)

    Zhu, Bin; Jiang, Lu; Cheng, Xuemei; Tao, Yang

    2005-11-01

    Machine vision methods are widely used in apple defect detection and quality grading applications. Currently, 2D near-infrared (NIR) imaging of apples is often used to detect apple defects because the image intensity of defects is different from normal apple parts. However, a drawback of this method is that the apple calyx also exhibits similar image intensity to the apple defects. Since an apple calyx often appears in the NIR image, the false alarm rate is high with the 2D NIR imaging method. In this paper, a 2D NIR imaging method is extended to a 3D reconstruction so that the apple calyx can be differentiated from apple defects according to their different 3D depth information. The Lambertian model is used to evaluate the reflectance map of the apple surface, and then Pentland's Shape-From-Shading (SFS) method is applied to reconstruct the 3D surface information of the apple based on Fast Fourier Transform (FFT). Pentland's method is directly derived from human perception properties, making it close to the way human eyes recover 3D information from a 2D scene. In addition, the FFT reduces the computation time significantly. The reconstructed 3D apple surface maps are shown in the results, and different depths of apple calyx and defects are obtained correctly.

  20. 3D laser inspection of fuel assembly grid spacers for nuclear reactors based on diffractive optical elements

    NASA Astrophysics Data System (ADS)

    Finogenov, L. V.; Lemeshko, Yu A.; Zav'yalov, P. S.; Chugui, Yu V.

    2007-06-01

    Ensuring the safety and high operation reliability of nuclear reactors takes 100% inspection of geometrical parameters of fuel assemblies, which include the grid spacers performed as a cellular structure with fuel elements. The required grid spacer geometry of assembly in the transverse and longitudinal cross sections is extremely important for maintaining the necessary heat regime. A universal method for 3D grid spacer inspection using a diffractive optical element (DOE), which generates as the structural illumination a multiple-ring pattern on the inner surface of a grid spacer cell, is investigated. Using some DOEs one can inspect the nomenclature of all produced grids. A special objective has been developed for forming the inner surface cell image. The problems of diffractive elements synthesis, projecting optics calculation, adjusting methods as well as calibration of the experimental measuring system are considered. The algorithms for image processing for different constructive elements of grids (cell, channel hole, outer grid spacer rim) and the experimental results are presented.

  1. 3D surface reconstruction and visualization of the Drosophila wing imaginal disc at cellular resolution

    NASA Astrophysics Data System (ADS)

    Bai, Linge; Widmann, Thomas; Jülicher, Frank; Dahmann, Christian; Breen, David

    2013-01-01

    Quantifying and visualizing the shape of developing biological tissues provide information about the morphogenetic processes in multicellular organisms. The size and shape of biological tissues depend on the number, size, shape, and arrangement of the constituting cells. To better understand the mechanisms that guide tissues into their final shape, it is important to investigate the cellular arrangement within tissues. Here we present a data processing pipeline to generate 3D volumetric surface models of epithelial tissues, as well as geometric descriptions of the tissues' apical cell cross-sections. The data processing pipeline includes image acquisition, editing, processing and analysis, 2D cell mesh generation, 3D contourbased surface reconstruction, cell mesh projection, followed by geometric calculations and color-based visualization of morphological parameters. In their first utilization we have applied these procedures to construct a 3D volumetric surface model at cellular resolution of the wing imaginal disc of Drosophila melanogaster. The ultimate goal of the reported effort is to produce tools for the creation of detailed 3D geometric models of the individual cells in epithelial tissues. To date, 3D volumetric surface models of the whole wing imaginal disc have been created, and the apicolateral cell boundaries have been identified, allowing for the calculation and visualization of cell parameters, e.g. apical cross-sectional area of cells. The calculation and visualization of morphological parameters show position-dependent patterns of cell shape in the wing imaginal disc. Our procedures should offer a general data processing pipeline for the construction of 3D volumetric surface models of a wide variety of epithelial tissues.

  2. Changes in quantitative 3D shape features of the optic nerve head associated with age

    NASA Astrophysics Data System (ADS)

    Christopher, Mark; Tang, Li; Fingert, John H.; Scheetz, Todd E.; Abramoff, Michael D.

    2013-02-01

    Optic nerve head (ONH) structure is an important biological feature of the eye used by clinicians to diagnose and monitor progression of diseases such as glaucoma. ONH structure is commonly examined using stereo fundus imaging or optical coherence tomography. Stereo fundus imaging provides stereo views of the ONH that retain 3D information useful for characterizing structure. In order to quantify 3D ONH structure, we applied a stereo correspondence algorithm to a set of stereo fundus images. Using these quantitative 3D ONH structure measurements, eigen structures were derived using principal component analysis from stereo images of 565 subjects from the Ocular Hypertension Treatment Study (OHTS). To evaluate the usefulness of the eigen structures, we explored associations with the demographic variables age, gender, and race. Using regression analysis, the eigen structures were found to have significant (p < 0.05) associations with both age and race after Bonferroni correction. In addition, classifiers were constructed to predict the demographic variables based solely on the eigen structures. These classifiers achieved an area under receiver operating characteristic curve of 0.62 in predicting a binary age variable, 0.52 in predicting gender, and 0.67 in predicting race. The use of objective, quantitative features or eigen structures can reveal hidden relationships between ONH structure and demographics. The use of these features could similarly allow specific aspects of ONH structure to be isolated and associated with the diagnosis of glaucoma, disease progression and outcomes, and genetic factors.

  3. 3D scanning electron microscopy applied to surface characterization of fluorosed dental enamel.

    PubMed

    Limandri, Silvina; Galván Josa, Víctor; Valentinuzzi, María Cecilia; Chena, María Emilia; Castellano, Gustavo

    2016-05-01

    The enamel surfaces of fluorotic teeth were studied by scanning electron stereomicroscopy. Different whitening treatments were applied to 25 pieces to remove stains caused by fluorosis and their surfaces were characterized by stereomicroscopy in order to obtain functional and amplitude parameters. The topographic features resulting for each treatment were determined through these parameters. The results obtained show that the 3D reconstruction achieved from the SEM stereo pairs is a valuable potential alternative for the surface characterization of this kind of samples.

  4. Generation of 3-D surface maps in waste storage silos using a structured light source

    NASA Technical Reports Server (NTRS)

    Burks, B. L.; Rowe, J. C.; Dinkins, M. A.; Christensen, B.; Selleck, C.; Jacoboski, D.; Markus, R.

    1992-01-01

    Surface contours inside the large waste storage tanks typical of the Department of Energy (DOE) complex are, in general, highly irregular. In addition to pipes and other pieces of equipment in the tanks, the surfaces may have features such as mounds, fissures, crystalline structures, and mixed solid and liquid forms. Prior to remediation activities, it will be necessary to characterize the waste to determine the most effective remediation approaches. Surface contour data will be required both prior to and during remediation. The use is described of a structured light source to generate 3-D surface contour maps of the interior of waste storage silos at the Feed Materials Production Center at Fernald, OH. The landscape inside these large waste storage tanks bears a strong resemblance to some of the landscapes that might be encountered during lunar or planetary exploration. Hence, these terrestrial 3-D mapping techniques may be directly applicable to extraterrestrial exploration. In further development, it will be demonstrated that these 3-D data can be used for robotic task planning just as 3-D surface contour data of a satellite could be used to plan maintenance tasks for a space-based servicing robot.

  5. Modeling Images of Natural 3D Surfaces: Overview and Potential Applications

    NASA Technical Reports Server (NTRS)

    Jalobeanu, Andre; Kuehnel, Frank; Stutz, John

    2004-01-01

    Generative models of natural images have long been used in computer vision. However, since they only describe the of 2D scenes, they fail to capture all the properties of the underlying 3D world. Even though such models are sufficient for many vision tasks a 3D scene model is when it comes to inferring a 3D object or its characteristics. In this paper, we present such a generative model, incorporating both a multiscale surface prior model for surface geometry and reflectance, and an image formation process model based on realistic rendering, the computation of the posterior model parameter densities, and on the critical aspects of the rendering. We also how to efficiently invert the model within a Bayesian framework. We present a few potential applications, such as asteroid modeling and Planetary topography recovery, illustrated by promising results on real images.

  6. Optical absorption enhancement in 3D nanofibers coated on polymer substrate for photovoltaic devices.

    PubMed

    Kiani, Amirkianoosh; Venkatakrishnan, Krishnan; Tan, Bo

    2015-06-01

    Recent research in the field of photovoltaics has shown that polymer solar cells have great potential to provide low-cost, lightweight and flexible electronic devices to harvest solar energy. In this paper, we propose a new method for the generation of three-dimensional nanofibers coated on polymer substrate induced by femtosecond laser pulses. In this new method, a thin layer of polymer is irradiated by megahertz femtosecond laser pulses under ambient conditions, and a thin fibrous layer is generated on top of the polymer substrate. This method is single step; no additional materials are added, and the layers of the three-dimensional (3D) polymer nanofibrous structures are grown on top of the substrate after laser irradiation. Light spectroscopy results show significant enhancement of light absorption in the generated 3D nanofibrous layers of polymer. Finally, we suggest how to maximize the light trapping and optical absorption of the generated nanofiber cells by optimizing the laser parameters.

  7. Three-Axis Distributed Fiber Optic Strain Measurement in 3D Woven Composite Structures

    NASA Technical Reports Server (NTRS)

    Castellucci, Matt; Klute, Sandra; Lally, Evan M.; Froggatt, Mark E.; Lowry, David

    2013-01-01

    Recent advancements in composite materials technologies have broken further from traditional designs and require advanced instrumentation and analysis capabilities. Success or failure is highly dependent on design analysis and manufacturing processes. By monitoring smart structures throughout manufacturing and service life, residual and operational stresses can be assessed and structural integrity maintained. Composite smart structures can be manufactured by integrating fiber optic sensors into existing composite materials processes such as ply layup, filament winding and three-dimensional weaving. In this work optical fiber was integrated into 3D woven composite parts at a commercial woven products manufacturing facility. The fiber was then used to monitor the structures during a VARTM manufacturing process, and subsequent static and dynamic testing. Low cost telecommunications-grade optical fiber acts as the sensor using a high resolution commercial Optical Frequency Domain Reflectometer (OFDR) system providing distributed strain measurement at spatial resolutions as low as 2mm. Strain measurements using the optical fiber sensors are correlated to resistive strain gage measurements during static structural loading. Keywords: fiber optic, distributed strain sensing, Rayleigh scatter, optical frequency domain reflectometry

  8. 3D surface imaging for guidance in breast cancer radiotherapy: organs at risk

    NASA Astrophysics Data System (ADS)

    Alderliesten, Tanja; Betgen, Anja; van Vliet-Vroegindeweij, Corine; Remeijer, Peter

    2013-03-01

    Purpose: To evaluate the variability in heart position in deep-inspiration breath-hold (DIBH) radiotherapy for breast cancer when 3D surface imaging would be used for monitoring the depth of the breath hold during treatment. Materials and Methods: Ten patients who received DIBH radiotherapy after breast-conserving surgery (BCS) were included. Retrospectively, heart-based registrations were performed for cone-beam computed tomography (CBCT) to planning CT and breast surface registrations were performed for a 3D surface (two different regions of interest [ROIs]), captured concurrently with CBCT, to planning CT. The resulting setup errors were compared with linear regression analysis and receiver operating characteristic (ROC) analysis was performed to investigate the prediction quality of 3D surface imaging for 3D heart displacement. Further, the residual setup errors (systematic [Σ] and random [σ]) of the heart were estimated relative to the surface registrations. Results: When surface imaging [ROIleft-side;ROIboth-sides] would be used for monitoring, the residual errors of the heart position are in left-right: Σ=[0.360.12], σ=[0.160.14] cranio-caudal: Σ=[0.540.54], σ=[0.280.31] and in anteriorposterior: Σ=[0.180.14], σ=[0.200.19] cm. Correlations between setup errors were: R2 = [0.23;0.73], [0.67;0.65], [0.65;0.73] in left-right, cranio-caudal, and anterior-posterior direction, respectively. ROC analysis resulted in an area under the ROC curve of [0.82;0.78]. Conclusion: The use of ROIboth-sides provided promising results. However, considerable variability in the heart position, particularly in CC direction, is observed when 3D surface imaging would be used for guidance in DIBH radiotherapy after BCS. Planning organ at risk volume margins should be used to take into account the heart-position variability.

  9. Efficient physics-based predictive 3D image modeling and simulation of optical atmospheric refraction phenomena

    NASA Astrophysics Data System (ADS)

    Reinhardt, Colin N.; Hammel, Stephen M.; Tsintikidis, Dimitris

    2016-09-01

    We present some preliminary results and discussion of our ongoing effort to develop a prototype volumetric atmospheric optical refraction simulator which uses 3D nonlinear ray-tracing and state-of-art physics-based rendering techniques. The tool will allow simulation of optical curved-ray propagation through nonlinear refractivity gradient profiles in volumetric atmospheric participating media, and the generation of radiometrically accurate images of the resulting atmospheric refraction phenomena, including inferior and superior mirages, over-the-horizon viewing conditions, looming and sinking, towering and stooping of distant objects. The ability to accurately model and predict atmospheric optical refraction conditions and phenomena is important in both defense and commercial applications. Our nonlinear refractive ray-trace method is currently CPU-parallelized and is well-suited for GPU compute implementation.

  10. The three-dimensional elemental distribution based on the surface topography by confocal 3D-XRF analysis

    NASA Astrophysics Data System (ADS)

    Yi, Longtao; Qin, Min; Wang, Kai; Lin, Xue; Peng, Shiqi; Sun, Tianxi; Liu, Zhiguo

    2016-09-01

    Confocal three-dimensional micro-X-ray fluorescence (3D-XRF) is a good surface analysis technology widely used to analyse elements and elemental distributions. However, it has rarely been applied to analyse surface topography and 3D elemental mapping in surface morphology. In this study, a surface adaptive algorithm using the progressive approximation method was designed to obtain surface topography. A series of 3D elemental mapping analyses in surface morphology were performed in laboratories to analyse painted pottery fragments from the Majiayao Culture (3300-2900 BC). To the best of our knowledge, for the first time, sample surface topography and 3D elemental mapping were simultaneously obtained. Besides, component and depth analyses were also performed using synchrotron radiation confocal 3D-XRF and tabletop confocal 3D-XRF, respectively. The depth profiles showed that the sample has a layered structure. The 3D elemental mapping showed that the red pigment, black pigment, and pottery coat contain a large amount of Fe, Mn, and Ca, respectively. From the 3D elemental mapping analyses at different depths, a 3D rendering was obtained, clearly showing the 3D distributions of the red pigment, black pigment, and pottery coat. Compared with conventional 3D scanning, this method is time-efficient for analysing 3D elemental distributions and hence especially suitable for samples with non-flat surfaces.

  11. Two-dimensional evaluation of 3D needled Cf/SiC composite fiber bundle surface

    NASA Astrophysics Data System (ADS)

    Wei, Jinhua; Lin, Bin; Cao, Xiaoyan; Zhang, Xiaofeng; Fang, Sheng

    2015-11-01

    The variations of fiber bundle surface microstructure have direct influence on the material performance, especially the friction and wear properties. Therefore, fiber bundle is the smallest evaluation unit of Cf/SiC composite surface. However, due to the anisotropy and inhomogeneity of Cf/SiC composite, it is difficult to evaluate the surface characteristics. Researchers think that two-dimensional evaluation is not suitable for the composites surface assessment any more because of its complex composition and varied surface structure. In this paper, a novel method is introduced for the evaluation of 3D needled Cf/SiC composite fiber bundle surface. On the level of Cf/SiC composite fiber bundle surface, two-dimensional evaluation method is adopted, with which the fiber bundle surface quality can be quantitatively evaluated by the two-dimensional surface roughness Ra. As long as the extracted surface profiles averagely distributed on Cf/SiC composite fiber bundle surface, with appropriate sampling length and sampling number, the mean value of Ra can estimate the whole surface roughness, thus reflecting the roughness degree of surface accurately. This study not only benefits the detection of 3D needled Cf/SiC composite fiber bundle surface quality, and lays a foundation on the evaluation of material functional features in further. And it corresponds to the convenient application in engineering practice.

  12. Controlled surface topography regulates collective 3D migration by epithelial-mesenchymal composite embryonic tissues.

    PubMed

    Song, Jiho; Shawky, Joseph H; Kim, YongTae; Hazar, Melis; LeDuc, Philip R; Sitti, Metin; Davidson, Lance A

    2015-07-01

    Cells in tissues encounter a range of physical cues as they migrate. Probing single cell and collective migratory responses to physically defined three-dimensional (3D) microenvironments and the factors that modulate those responses are critical to understanding how tissue migration is regulated during development, regeneration, and cancer. One key physical factor that regulates cell migration is topography. Most studies on surface topography and cell mechanics have been carried out with single migratory cells, yet little is known about the spreading and motility response of 3D complex multi-cellular tissues to topographical cues. Here, we examine the response to complex topographical cues of microsurgically isolated tissue explants composed of epithelial and mesenchymal cell layers from naturally 3D organized embryos of the aquatic frog Xenopus laevis. We control topography using fabricated micropost arrays (MPAs) and investigate the collective 3D migration of these multi-cellular systems in these MPAs. We find that the topography regulates both collective and individual cell migration and that dense MPAs reduce but do not eliminate tissue spreading. By modulating cell size through the cell cycle inhibitor Mitomycin C or the spacing of the MPAs we uncover how 3D topographical cues disrupt collective cell migration. We find surface topography can direct both single cell motility and tissue spreading, altering tissue-scale processes that enable efficient conversion of single cell motility into collective movement.

  13. 3D-modeling of Callisto's sputtered surface-exosphere environment

    NASA Astrophysics Data System (ADS)

    Lammer, Helmut; Pfleger, Martin; Lindqvist, Jesper; Lichtenegger, Herbert; Holmström, Mats; Vorburger, Audrey; Wurz, Peter; Barabash, Stas

    2016-04-01

    We study the stoichiometrical release of various surface elements caused by plasma sputtering from an assumed icy and non-icy (i.e., chondritic) surface into the exosphere of the Jovian satellite Callisto. We apply a 3D plasma planetary interaction hybrid model that is used for the evaluation of precipitation maps of magnetospheric H+, O+ and S+ sputter agents onto Callisto's surface. The obtained precipitation maps are then applied to the assumed surface compositions where the related sputter yields are calculated by means of the 2013 SRIM code and are coupled with a 3D exosphere model. Sputtered surface particles are followed on their individual trajectories until they either escape Callisto's gravitational attraction or return to the surface. We study also the effect of collisions between sputter species and ambient O2 molecules which form a tiny atmosphere near the satellite's surface and compare the exosphere densities that are obtained from the 3D model with and without a background gaseous envelope with recent 1D model results. Finally we discuss if the Neutral gas and Ion Mass (NIM) spectrometer, that is part of the Particle Environment Package (PEP) on board of the JUICE mission will be able to detect sputtered particles from Callisto's icy and non-icy surface.

  14. The use of 3D surface scanning for the measurement and assessment of the human foot

    PubMed Central

    2010-01-01

    Background A number of surface scanning systems with the ability to quickly and easily obtain 3D digital representations of the foot are now commercially available. This review aims to present a summary of the reported use of these technologies in footwear development, the design of customised orthotics, and investigations for other ergonomic purposes related to the foot. Methods The PubMed and ScienceDirect databases were searched. Reference lists and experts in the field were also consulted to identify additional articles. Studies in English which had 3D surface scanning of the foot as an integral element of their protocol were included in the review. Results Thirty-eight articles meeting the search criteria were included. Advantages and disadvantages of using 3D surface scanning systems are highlighted. A meta-analysis of studies using scanners to investigate the changes in foot dimensions during varying levels of weight bearing was carried out. Conclusions Modern 3D surface scanning systems can obtain accurate and repeatable digital representations of the foot shape and have been successfully used in medical, ergonomic and footwear development applications. The increasing affordability of these systems presents opportunities for researchers investigating the foot and for manufacturers of foot related apparel and devices, particularly those interested in producing items that are customised to the individual. Suggestions are made for future areas of research and for the standardization of the protocols used to produce foot scans. PMID:20815914

  15. New light-trapping concept by means of several optical components applied to compact holographic 3D concentration solar module

    NASA Astrophysics Data System (ADS)

    Villamarín Villegas, Ayalid M.; Pérez López, Francisco J.; Calo López, Antonio; Rodríguez San Segundo, Hugo-José

    2014-05-01

    A new light-trapping concept is presented, which joins broad bandwidth volume phase reflection holograms (VPRH) working together with three other optical components: specifically designed three-dimensional (3D) cavities, Total Internal Reflection (TIR) within an optical medium, and specular reflection by means of a highly reflective surface. This concept is applied to the design and development of both low concentration photovoltaic (LCPV) and solar thermal modules reaching a concentration factor of up to 3X. Higher concentrations are feasible for use in concentrated solar power (CSP) devices. The whole system is entirely made of polymeric materials (except for the solar cells or fluid carrying pipes), thus reducing cost by up to 40%. The module concentrates solar light onto solar cells - or fluid carrying pipes - with no need for active tracking of the sun, covering the whole seasonal and daily incident angle spectrum while it also minimizes optical losses. In this work we analyze the first experimentally measured optical characteristics and performance of VPRH in dichromated gelatin film (DCG) in our concept. The VPRH can reach high diffraction efficiencies (˜98%, ignoring Fresnel reflection losses). Thanks to specifically designed raw material, coating and developing process specifications, also very broad selective spectral (higher than 300 nm) and angular bandwidths (˜+20º) per grating are achieved. The VPRH was optimized to use silicon solar cells, but designs for other semiconductor devices or for fluid heating are feasible. The 3D shape, the hologram's and reflective surface's optical quality, the TIR effect and the correct coupling of all the components are key to high performance of the concentration solar module.

  16. Operational Retrieval of aerosol optical depth over Indian subcontinent and Indian Ocean using INSAT-3D/Imager product validation

    NASA Astrophysics Data System (ADS)

    Mishra, M. K.; Rastogi, G.; Chauhan, P.

    2014-11-01

    Aerosol optical depth (AOD) over Indian subcontinent and Indian Ocean region is derived operationally for the first time from the geostationary earth orbit (GEO) satellite INSAT-3D Imager data at 0.65 μm wavelength. Single visible channel algorithm based on clear sky composites gives larger retrieval error in AOD than other multiple channel algorithms due to errors in estimating surface reflectance and atmospheric property. However, since MIR channel signal is insensitive to the presence of most aerosols, therefore in present study, AOD retrieval algorithm employs both visible (centred at 0.65 μm) and mid-infrared (MIR) band (centred at 3.9 μm) measurements, and allows us to monitor transport of aerosols at higher temporal resolution. Comparisons made between INSAT-3D derived AOD (τI) and MODIS derived AOD (τM) co-located in space (at 1° resolution) and time during January, February and March (JFM) 2014 encompasses 1165, 1052 and 900 pixels, respectively. Good agreement found between τI and τM during JFM 2014 with linear correlation coefficients (R) of 0.87, 0.81 and 0.76, respectively. The extensive validation made during JFM 2014 encompasses 215 co-located AOD in space and time derived by INSAT 3D (τI) and 10 sun-photometers (τA) that includes 9 AERONET (Aerosol Robotic Network) and 1 handheld sun-photometer site. INSAT-3D derived AOD i.e. τI, is found within the retrieval errors of τI = ±0.07 ±0.15τA with linear correlation coefficient (R) of 0.90 and root mean square error equal (RMSE) to 0.06. Present work shows that INSAT-3D aerosol products can be used quantitatively in many applications with caution for possible residual clouds, snow/ice, and water contamination.

  17. Incorporating 3D body motions into large-sized freeform surface conceptual design.

    PubMed

    Qin, Shengfeng; Wright, David K; Kang, Jingsheng; Prieto, P A

    2005-01-01

    Large-sized free-form surface design presents some challenges in practice. Especially at the conceptual design stage, sculpting physical models is still essential for surface development, because CAD models are less intuitive for designers to design and modify them. These sculpted physical models can be then scanned and converted into CAD models. However, if the physical models are too big, designers may have problems in finding a suitable position to conduct their operations or simply the models are difficult to be scanned in. We investigated a novel surface modelling approach by utilising a 3D motion capture system. For designing a large-sized surface, a network of splines is initially set up. Artists or designers wearing motion marks on their hands can then change shapes of the splines with their hands. Literarily they can move their body freely to any positions to perform their tasks. They can also move their hands in 3D free space to detail surface characteristics by their gestures. All their design motions are recorded in the motion capturing system and transferred into 3D curves and surfaces correspondingly. This paper reports this novel surface design method associated with some case studies.

  18. Active illumination based 3D surface reconstruction and registration for image guided medialization laryngoplasty

    NASA Astrophysics Data System (ADS)

    Jin, Ge; Lee, Sang-Joon; Hahn, James K.; Bielamowicz, Steven; Mittal, Rajat; Walsh, Raymond

    2007-03-01

    The medialization laryngoplasty is a surgical procedure to improve the voice function of the patient with vocal fold paresis and paralysis. An image guided system for the medialization laryngoplasty will help the surgeons to accurately place the implant and thus reduce the failure rates of the surgery. One of the fundamental challenges in image guided system is to accurately register the preoperative radiological data to the intraoperative anatomical structure of the patient. In this paper, we present a combined surface and fiducial based registration method to register the preoperative 3D CT data to the intraoperative surface of larynx. To accurately model the exposed surface area, a structured light based stereo vision technique is used for the surface reconstruction. We combined the gray code pattern and multi-line shifting to generate the intraoperative surface of the larynx. To register the point clouds from the intraoperative stage to the preoperative 3D CT data, a shape priori based ICP method is proposed to quickly register the two surfaces. The proposed approach is capable of tracking the fiducial markers and reconstructing the surface of larynx with no damage to the anatomical structure. We used off-the-shelf digital cameras, LCD projector and rapid 3D prototyper to develop our experimental system. The final RMS error in the registration is less than 1mm.

  19. 3D surface topography of cylinder liner forecasting during plateau honing process

    NASA Astrophysics Data System (ADS)

    Reizer, R.; Pawlus, P.

    2011-08-01

    Areal surface topographies after plateau honing process were measured. A correlation analysis of surface texture parameters was then carried out. As the results, the following parameters describing plateau honed cylinder 3D surface topography were selected: amplitude Sq, Sz, spatial: Str, Std, hybrid SΔq as well as functional: Spq, Svq and Smq. 3D surface topographies were modeled. The modeled surface topographies were correctly matched to measured ones in 77% of all analyzed cases. The plateau honing experiment was then carried out using an orthogonal selective research plan. Two machining parameters were input variables: coarse honing pressure pv and plateau honing time t. Chosen cylinder liners texture parameters were output values. As the result of the experiment, regression equations connecting plateau honing process parameters pv and t with recommended 3D surface topography parameters were obtained. Finally, cylinder liner surface topographies were predicted for various values of machining parameters. Proper matching accuracy of modeled to measured textures was assured in 67% of analyzed cases.

  20. 3D imaging of translucent media with a plenoptic sensor based on phase space optics

    NASA Astrophysics Data System (ADS)

    Zhang, Xuanzhe; Shu, Bohong; Du, Shaojun

    2015-05-01

    Traditional stereo imaging technology is not working for dynamical translucent media, because there are no obvious characteristic patterns on it and it's not allowed using multi-cameras in most cases, while phase space optics can solve the problem, extracting depth information directly from "space-spatial frequency" distribution of the target obtained by plenoptic sensor with single lens. This paper discussed the presentation of depth information in phase space data, and calculating algorithms with different transparency. A 3D imaging example of waterfall was given at last.

  1. Fiber optic vibration sensor for high-power electric machines realized using 3D printing technology

    NASA Astrophysics Data System (ADS)

    Igrec, Bojan; Bosiljevac, Marko; Sipus, Zvonimir; Babic, Dubravko; Rudan, Smiljko

    2016-03-01

    The objective of this work was to demonstrate a lightweight and inexpensive fiber-optic vibration sensor, built using 3D printing technology, for high-power electric machines and similar applications. The working principle is based on modulating the light intensity using a blade attached to a bendable membrane. The sensor prototype was manufactured using PolyJet Matrix technology with DM 8515 Grey 35 Polymer. The sensor shows linear response, expected bandwidth (< 150 Hz), and from our measurements we estimated the damping ratio for used polymer to be ζ ≍ 0.019. The developed prototype is simple to assemble, adjust, calibrate and repair.

  2. Optimal Image Stitching for Concrete Bridge Bottom Surfaces Aided by 3d Structure Lines

    NASA Astrophysics Data System (ADS)

    Liu, Yahui; Yao, Jian; Liu, Kang; Lu, Xiaohu; Xia, Menghan

    2016-06-01

    Crack detection for bridge bottom surfaces via remote sensing techniques is undergoing a revolution in the last few years. For such applications, a large amount of images, acquired with high-resolution industrial cameras close to the bottom surfaces with some mobile platform, are required to be stitched into a wide-view single composite image. The conventional idea of stitching a panorama with the affine model or the homographic model always suffers a series of serious problems due to poor texture and out-of-focus blurring introduced by depth of field. In this paper, we present a novel method to seamlessly stitch these images aided by 3D structure lines of bridge bottom surfaces, which are extracted from 3D camera data. First, we propose to initially align each image in geometry based on its rough position and orientation acquired with both a laser range finder (LRF) and a high-precision incremental encoder, and these images are divided into several groups with the rough position and orientation data. Secondly, the 3D structure lines of bridge bottom surfaces are extracted from the 3D cloud points acquired with 3D cameras, which impose additional strong constraints on geometrical alignment of structure lines in adjacent images to perform a position and orientation optimization in each group to increase the local consistency. Thirdly, a homographic refinement between groups is applied to increase the global consistency. Finally, we apply a multi-band blending algorithm to generate a large-view single composite image as seamlessly as possible, which greatly eliminates both the luminance differences and the color deviations between images and further conceals image parallax. Experimental results on a set of representative images acquired from real bridge bottom surfaces illustrate the superiority of our proposed approaches.

  3. Controlled implant/soft tissue interaction by nanoscale surface modifications of 3D porous titanium implants.

    PubMed

    Rieger, Elisabeth; Dupret-Bories, Agnès; Salou, Laetitia; Metz-Boutigue, Marie-Helene; Layrolle, Pierre; Debry, Christian; Lavalle, Philippe; Vrana, Nihal Engin

    2015-06-07

    Porous titanium implants are widely employed in the orthopaedics field to ensure good bone fixation. Recently, the use of porous titanium implants has also been investigated in artificial larynx development in a clinical setting. Such uses necessitate a better understanding of the interaction of soft tissues with porous titanium structures. Moreover, surface treatments of titanium have been generally evaluated in planar structures, while the porous titanium implants have complex 3 dimensional (3D) architectures. In this study, the determining factors for soft tissue integration of 3D porous titanium implants were investigated as a function of surface treatments via quantification of the interaction of serum proteins and cells with single titanium microbeads (300-500 μm in diameter). Samples were either acid etched or nanostructured by anodization. When the samples are used in 3D configuration (porous titanium discs of 2 mm thickness) in vivo (in subcutis of rats for 2 weeks), a better integration was observed for both anodized and acid etched samples compared to the non-treated implants. If the implants were also pre-treated with rat serum before implantation, the integration was further facilitated. In order to understand the underlying reasons for this effect, human fibroblast cell culture tests under several conditions (directly on beads, beads in suspension, beads encapsulated in gelatin hydrogels) were conducted to mimic the different interactions of cells with Ti implants in vivo. Physical characterization showed that surface treatments increased hydrophilicity, protein adsorption and roughness. Surface treatments also resulted in improved adsorption of serum albumin which in turn facilitated the adsorption of other proteins such as apolipoprotein as quantified by protein sequencing. The cellular response to the beads showed considerable difference with respect to the cell culture configuration. When the titanium microbeads were entrapped in cell

  4. Sensitivity study of 3-D modeling for multi-D inversion of surface NMR

    NASA Astrophysics Data System (ADS)

    Warsa, Grandis, Hendra

    2012-06-01

    Geophysical field method of surface nuclear magnetic resonance (SNMR) allows a direct determination of hydrogeological parameters of the subsurface. The amplitude of the SNMR signal is directly linked to the amount of mobile water. The relaxation behaviour of the signal correlates with pore sizes and hydraulic conductivities of an aquifer. For improving capability and reliability of SNMR method we have presented a forward modeling scheme of 3-D water content and decay time structures that can be used for multi-D interpretation. Currently SNMR is carried out mainly with a 1-D working scheme using coinciding loops. For each sounding point using a coincident circular loop antenna, the amplitudes and decay times of the SNMR signal are the product of a three dimensional distribution of the water content and decay time in the subsurface and their sensitivity to the receiver. The antenna is moved at the surface and the SNMR relaxation signal are plotted as a function of the pulse moment and sounding point. The errors might be very large by neglecting the 2-D or even 3-D geometry of the structures which have to be considered in the analysis and inversion in the future. The results show that the 3-D modeling is reliable and flexible to be integrated into the 2-D/3-D inversion scheme for inverting surface NMR data to recover a multi-D distribution of water content and decay time of an aquifer.

  5. Polymer optical fibers integrated directly into 3D orthogonal woven composites for sensing

    NASA Astrophysics Data System (ADS)

    Hamouda, Tamer; Seyam, Abdel-Fattah M.; Peters, Kara

    2015-02-01

    This study demonstrates that standard polymer optical fibers (POF) can be directly integrated into composites from 3D orthogonal woven preforms during the weaving process and then serve as in-situ sensors to detect damage due to bending or impact loads. Different composite samples with embedded POF were fabricated of 3D orthogonal woven composites with different parameters namely number of y-/x-layers and x-yarn density. The signal of POF was not affected significantly by the preform structure. During application of resin using VARTM technique, significant drop in backscattering level was observed due to pressure caused by vacuum on the embedded POF. Measurements of POF signal while in the final composites after resin cure indicated that the backscattering level almost returned to the original level of un-embedded POF. The POF responded to application of bending and impact loads to the composite with a reduction in the backscattering level. The backscattering level almost returned back to its original level after removing the bending load until damage was present in the composite. Similar behavior occurred due to impact events. As the POF itself is used as the sensor and can be integrated throughout the composite, large sections of future 3D woven composite structures could be monitored without the need for specialized sensors or complex instrumentation.

  6. Hydrothermal synthesis, characterization and optical properties of 3D flower like indium sulfide nanostructures

    NASA Astrophysics Data System (ADS)

    Ghaderi Sheikhi abadi, Parvaneh; Salavati-Niasari, Masoud; Davar, Fatemeh

    2013-01-01

    High-quality and high-yield 3D flower like indium sulfide (In2S3) nanostructures with cubic structure were synthesized by a wet chemical route, without using any surfactant and organic solvents at 160 °C for 12 h, by using InCl3 and 2-aminothiophenol (2-ATP) as starting reagents. The obtained In2S3 with different morphologies and size was characterized by X-ray diffraction pattern (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), and ultraviolet-visible (UV-vis) spectroscopy. The effects of reaction parameters, such as temperature, precursor concentration and reaction time on the morphology, and particle size of products were investigated. Our experimental results showed that temperature and time reaction played key roles in the final morphology of In2S3. The morphology of In2S3 structures could be changed from one-dimensional (1D) structures to three-dimensional (3D) structures by increasing reaction time to 24 h. In the present study the optical properties 3D In2S3 structures were investigated.

  7. 3D optical sectioning with a new hyperspectral confocal fluorescence imaging system.

    SciTech Connect

    Nieman, Linda T.; Sinclair, Michael B.; Davidson, George S.; Van Benthem, Mark Hilary; Haaland, David Michael; Timlin, Jerilyn Ann; Sasaki, Darryl Yoshio; Bachand, George David; Jones, Howland D. T.

    2007-02-01

    A novel hyperspectral fluorescence microscope for high-resolution 3D optical sectioning of cells and other structures has been designed, constructed, and used to investigate a number of different problems. We have significantly extended new multivariate curve resolution (MCR) data analysis methods to deconvolve the hyperspectral image data and to rapidly extract quantitative 3D concentration distribution maps of all emitting species. The imaging system has many advantages over current confocal imaging systems including simultaneous monitoring of numerous highly overlapped fluorophores, immunity to autofluorescence or impurity fluorescence, enhanced sensitivity, and dramatically improved accuracy, reliability, and dynamic range. Efficient data compression in the spectral dimension has allowed personal computers to perform quantitative analysis of hyperspectral images of large size without loss of image quality. We have also developed and tested software to perform analysis of time resolved hyperspectral images using trilinear multivariate analysis methods. The new imaging system is an enabling technology for numerous applications including (1) 3D composition mapping analysis of multicomponent processes occurring during host-pathogen interactions, (2) monitoring microfluidic processes, (3) imaging of molecular motors and (4) understanding photosynthetic processes in wild type and mutant Synechocystis cyanobacteria.

  8. Variation in the measurement of cranial volume and surface area using 3D laser scanning technology.

    PubMed

    Sholts, Sabrina B; Wärmländer, Sebastian K T S; Flores, Louise M; Miller, Kevin W P; Walker, Phillip L

    2010-07-01

    Three-dimensional (3D) laser scanner models of human crania can be used for forensic facial reconstruction, and for obtaining craniometric data useful for estimating age, sex, and population affinity of unidentified human remains. However, the use of computer-generated measurements in a casework setting requires the measurement precision to be known. Here, we assess the repeatability and precision of cranial volume and surface area measurements using 3D laser scanner models created by different operators using different protocols for collecting and processing data. We report intraobserver measurement errors of 0.2% and interobserver errors of 2% of the total area and volume values, suggesting that observer-related errors do not pose major obstacles for sharing, combining, or comparing such measurements. Nevertheless, as no standardized procedure exists for area or volume measurements from 3D models, it is imperative to report the scanning and postscanning protocols employed when such measurements are conducted in a forensic setting.

  9. 3D Surface Reconstruction of Plant Seeds by Volume Carving: Performance and Accuracies

    PubMed Central

    Roussel, Johanna; Geiger, Felix; Fischbach, Andreas; Jahnke, Siegfried; Scharr, Hanno

    2016-01-01

    We describe a method for 3D reconstruction of plant seed surfaces, focusing on small seeds with diameters as small as 200 μm. The method considers robotized systems allowing single seed handling in order to rotate a single seed in front of a camera. Even though such systems feature high position repeatability, at sub-millimeter object scales, camera pose variations have to be compensated. We do this by robustly estimating the tool center point from each acquired image. 3D reconstruction can then be performed by a simple shape-from-silhouette approach. In experiments we investigate runtimes, theoretically achievable accuracy, experimentally achieved accuracy, and show as a proof of principle that the proposed method is well sufficient for 3D seed phenotyping purposes. PMID:27375628

  10. DLP/DSP-based optical 3D sensors for the mass market in industrial metrology and life sciences

    NASA Astrophysics Data System (ADS)

    Frankowski, G.; Hainich, R.

    2011-03-01

    GFM has developed and constructed DLP-based optical 3D measuring devices based on structured light illumination. Over the years the devices have been used in industrial metrology and life sciences for different 3D measuring tasks. This lecture will discuss integration of DLP Pico technology and DSP technology from Texas Instruments for mass market optical 3D sensors. In comparison to existing mass market laser triangulation sensors, the new 3D sensors provide a full-field measurement of up to a million points in less than a second. The lecture will further discuss different fields of application and advantages of the new generation of 3D sensors for: OEM application in industrial measuring and inspection; 3D metrology in industry, life sciences and biometrics, and industrial image processing.

  11. SU-F-BRF-08: Conformal Mapping-Based 3D Surface Matching and Registration

    SciTech Connect

    Song, Y; Zeng, W; Gu, X; Liu, C

    2014-06-15

    Purpose: Recently, non-rigid 3D surface matching and registration has been used extensively in engineering and medicine. However, matching 3D surfaces undergoing non-rigid deformation accurately is still a challenging mathematical problem. In this study, we present a novel algorithm to address this issue by introducing intrinsic symmetry to the registration Methods: Our computational algorithm for symmetric conformal mapping is divided into three major steps: 1) Finding the symmetric plane; 2) Finding feature points; and 3) Performing cross registration. The key strategy is to preserve the symmetry during the conformal mapping, such that the image on the parameter domain is symmetric and the area distortion factor on the parameter image is also symmetric. Several novel algorithms were developed using different conformal geometric tools. One was based on solving Riemann-Cauchy equation and the other one employed curvature flow Results: Our algorithm was implemented using generic C++ on Windows XP and used conjugate gradient search optimization for acceleration. The human face 3D surface images were acquired using a high speed 3D scanner based on the phase-shifting method. The scanning speed was 30 frames/sec. The image resolution for each frame was 640 × 480. For 3D human face surfaces with different expressions, postures, and boundaries, our algorithms were able to produce consistent result on the texture pattern on the overlapping region Conclusion: We proposed a novel algorithm to improve the robustness of conformal geometric methods by incorporating the symmetric information into the mapping process. To objectively evaluate its performance, we compared it with most existing techniques. Experimental results indicated that our method outperformed all the others in terms of robustness. The technique has a great potential in real-time patient monitoring and tracking in image-guided radiation therapy.

  12. New optical four-quadrant phase detector integrated into a photogate array for small and precise 3D cameras

    NASA Astrophysics Data System (ADS)

    Schwarte, Rudolf; Xu, Zhanping; Heinol, Horst-Guenther; Olk, Joachim; Buxbaum, Bernd

    1997-03-01

    The photonic mixer device (PMD) is a new electro-optical mixing semiconductor device. Integrated into a line or an array it may contribute a significant improvement in developing an extremely fast, flexible, robust and low cost 3D-solid-state camera. Three dimensional (3D)-cameras are of dramatically increasing interest in industrial automation, especially for production integrated quality control, in- house navigation, etc. The type of 3D-camera here under consideration is based on the principle of time-of-flight respectively phase delay of surface reflected echoes of rf- modulated light. In contrast to 3D-laser radars there is no scanner required since the whole 3D-scene is illuminated simultaneously using intensity-modulated incoherent light, e.g. in the 10 to 1000 MHz range. The rf-modulated light reflected from the 3D-scene represents the total depth information within the local delay of the back scattered phase front. If this incoming wave front is again rf- modulated by a 2D-mixer within the whole receiving aperture we get a quasi-stationary rf-inference pattern respectively rf-interferogram which may be captured by means of a conventional CCD-camera. This procedure is called rf- modulation interferometry (RFMI). Corresponding to first simulative results the new PMD-array will be appropriate to the RFMI-procedure. Though looking like a modified CCD-array or CMOS-photodetector array it will be able to perform both, the pixelwise mixing process for phase delay respectively depth evaluation and the pixelwise light intensity acquisition for gray level or color evaluation. Further advantageous properties are achieved by means of a four- quadrant (4Q)-PMD array which operates as a balanced inphase/quadrature phase (I/Q)-mixer and will be able to capture the total 3D-scene information of several 100,000 voxels within the microsecond(s) - to ms-range.

  13. Three-axis distributed fiber optic strain measurement in 3D woven composite structures

    NASA Astrophysics Data System (ADS)

    Castellucci, Matt; Klute, Sandra; Lally, Evan M.; Froggatt, Mark E.; Lowry, David

    2013-03-01

    Recent advancements in composite materials technologies have broken further from traditional designs and require advanced instrumentation and analysis capabilities. Success or failure is highly dependent on design analysis and manufacturing processes. By monitoring smart structures throughout manufacturing and service life, residual and operational stresses can be assessed and structural integrity maintained. Composite smart structures can be manufactured by integrating fiber optic sensors into existing composite materials processes such as ply layup, filament winding and three-dimensional weaving. In this work optical fiber was integrated into 3D woven composite parts at a commercial woven products manufacturing facility. The fiber was then used to monitor the structures during a VARTM manufacturing process, and subsequent static and dynamic testing. Low cost telecommunications-grade optical fiber acts as the sensor using a high resolution commercial Optical Frequency Domain Reflectometer (OFDR) system providing distributed strain measurement at spatial resolutions as low as 2mm. Strain measurements using the optical fiber sensors are correlated to resistive strain gage measurements during static structural loading.

  14. Optically clearing tissue as an initial step for 3D imaging of core biopsies to diagnose pancreatic cancer

    NASA Astrophysics Data System (ADS)

    Das, Ronnie; Agrawal, Aishwarya; Upton, Melissa P.; Seibel, Eric J.

    2014-02-01

    The pancreas is a deeply seated organ requiring endoscopically, or radiologically guided biopsies for tissue diagnosis. Current approaches include either fine needle aspiration biopsy (FNA) for cytologic evaluation, or core needle biopsies (CBs), which comprise of tissue cores (L = 1-2 cm, D = 0.4-2.0 mm) for examination by brightfield microscopy. Between procurement and visualization, biospecimens must be processed, sectioned and mounted on glass slides for 2D visualization. Optical information about the native tissue state can be lost with each procedural step and a pathologist cannot appreciate 3D organization from 2D observations of tissue sections 1-8 μm in thickness. Therefore, how might histological disease assessment improve if entire, intact CBs could be imaged in both brightfield and 3D? CBs are mechanically delicate; therefore, a simple device was made to cut intact, simulated CBs (L = 1-2 cm, D = 0.2-0.8 mm) from porcine pancreas. After CBs were laid flat in a chamber, z-stack images at 20x and 40x were acquired through the sample with and without the application of an optical clearing agent (FocusClear®). Intensity of transmitted light increased by 5-15x and islet structures unique to pancreas were clearly visualized 250-300 μm beneath the tissue surface. CBs were then placed in index matching square capillary tubes filled with FocusClear® and a standard optical clearing agent. Brightfield z-stack images were then acquired to present 3D visualization of the CB to the pathologist.

  15. Planned development of a 3D computer based on free-space optical interconnects

    NASA Astrophysics Data System (ADS)

    Neff, John A.; Guarino, David R.

    1994-05-01

    Free-space optical interconnection has the potential to provide upwards of a million data channels between planes of electronic circuits. This may result in the planar board and backplane structures of today giving away to 3-D stacks of wafers or multi-chip modules interconnected via channels running perpendicular to the processor planes, thereby eliminating much of the packaging overhead. Three-dimensional packaging is very appealing for tightly coupled fine-grained parallel computing where the need for massive numbers of interconnections is severely taxing the capabilities of the planar structures. This paper describes a coordinated effort by four research organizations to demonstrate an operational fine-grained parallel computer that achieves global connectivity through the use of free space optical interconnects.

  16. Respiratory motion correction in 3-D PET data with advanced optical flow algorithms.

    PubMed

    Dawood, Mohammad; Buther, Florian; Jiang, Xiaoyi; Schafers, Klaus P

    2008-08-01

    The problem of motion is well known in positron emission tomography (PET) studies. The PET images are formed over an elongated period of time. As the patients cannot hold breath during the PET acquisition, spatial blurring and motion artifacts are the natural result. These may lead to wrong quantification of the radioactive uptake. We present a solution to this problem by respiratory-gating the PET data and correcting the PET images for motion with optical flow algorithms. The algorithm is based on the combined local and global optical flow algorithm with modifications to allow for discontinuity preservation across organ boundaries and for application to 3-D volume sets. The superiority of the algorithm over previous work is demonstrated on software phantom and real patient data.

  17. Generating 3D and 3D-like animations of strongly uneven surface microareas of bloodstains from small series of partially out-of-focus digital SEM micrographs.

    PubMed

    Hortolà, Policarp

    2010-01-01

    When dealing with microscopic still images of some kinds of samples, the out-of-focus problem represents a particularly serious limiting factor for the subsequent generation of fully sharp 3D animations. In order to produce fully-focused 3D animations of strongly uneven surface microareas, a vertical stack of six digital secondary-electron SEM micrographs of a human bloodstain microarea was acquired. Afterwards, single combined images were generated using a macrophotography and light microscope image post-processing software. Subsequently, 3D animations of texture and topography were obtained in different formats using a combination of software tools. Finally, a 3D-like animation of a texture-topography composite was obtained in different formats using another combination of software tools. By one hand, results indicate that the use of image post-processing software not concerned primarily with electron micrographs allows to obtain, in an easy way, fully-focused images of strongly uneven surface microareas of bloodstains from small series of partially out-of-focus digital SEM micrographs. On the other hand, results also indicate that such small series of electron micrographs can be utilized for generating 3D and 3D-like animations that can subsequently be converted into different formats, by using certain user-friendly software facilities not originally designed for use in SEM, that are easily available from Internet. Although the focus of this study was on bloodstains, the methods used in it well probably are also of relevance for studying the surface microstructures of other organic or inorganic materials whose sharp displaying is difficult of obtaining from a single SEM micrograph.

  18. 3D Micropatterned Surface Inspired by Salvinia molesta via Direct Laser Lithography

    PubMed Central

    2015-01-01

    Biomimetic functional surfaces are attracting increasing attention for their relevant technological applications. Despite these efforts, inherent limitations of microfabrication techniques prevent the replication of complex hierarchical microstructures. Using a 3D laser lithography technique, we fabricated a 3D patterned surface bioinspired to Salvinia molesta leaves. The artificial hairs, with crownlike heads, were reproduced by scaling down (ca. 100 times smaller) the dimensions of natural features, so that microscale hairs with submicrometric resolution were attained. The micropatterned surface, in analogy with the natural model, shows interesting properties in terms of hydrophobicity and air retention when submerged by water, even if realized with a hydrophilic material. Furthermore, we successfully demonstrated the capability to promote localized condensation of water droplets from moisture in the atmosphere. PMID:26558410

  19. 3D Micropatterned Surface Inspired by Salvinia molesta via Direct Laser Lithography.

    PubMed

    Tricinci, Omar; Terencio, Tercio; Mazzolai, Barbara; Pugno, Nicola M; Greco, Francesco; Mattoli, Virgilio

    2015-11-25

    Biomimetic functional surfaces are attracting increasing attention for their relevant technological applications. Despite these efforts, inherent limitations of microfabrication techniques prevent the replication of complex hierarchical microstructures. Using a 3D laser lithography technique, we fabricated a 3D patterned surface bioinspired to Salvinia molesta leaves. The artificial hairs, with crownlike heads, were reproduced by scaling down (ca. 100 times smaller) the dimensions of natural features, so that microscale hairs with submicrometric resolution were attained. The micropatterned surface, in analogy with the natural model, shows interesting properties in terms of hydrophobicity and air retention when submerged by water, even if realized with a hydrophilic material. Furthermore, we successfully demonstrated the capability to promote localized condensation of water droplets from moisture in the atmosphere.

  20. Anomalous Surface Deformation of Sapphire Clarified by 3D-FEM Simulation of the Nanoindentation

    NASA Astrophysics Data System (ADS)

    Nowak, Roman; Manninen, Timo; Li, Chunliang; Heiskanen, Kari; Hannula, Simo-Pekka; Lindroos, Veikko; Soga, Tetsuo; Yoshida, Fusahito

    This work clarifies the origin of anomalous surface deformation reflected by peculiar surface patterns around indentation impressions on various crystallographic planes of sapphire. The three-dimensional finite element simulation (3D-FEM) of nanoindentation in Al2O3 crystal allowed the authors to localize the regions in which various kinds of twinning and slip are most prone to be activated. The work provides a novel approach to the “hardness anisotropy”, which was modeled so far using a modified uniaxial-stress approximation of this essentially 3D, non-isotropic contact problem. The calculated results enabled the authors to unravel the asymmetric surface deformation detected on prismatic planes by the high-resolution microscopy, which cannot be explained using simple crystallographic considerations.

  1. Optical-CT 3D Dosimetry Using Fresnel Lenses with Minimal Refractive-Index Matching Fluid

    PubMed Central

    Bache, Steven; Malcolm, Javian; Adamovics, John; Oldham, Mark

    2016-01-01

    Telecentric optical computed tomography (optical-CT) is a state-of-the-art method for visualizing and quantifying 3-dimensional dose distributions in radiochromic dosimeters. In this work a prototype telecentric system (DFOS—Duke Fresnel Optical-CT Scanner) is evaluated which incorporates two substantial design changes: the use of Fresnel lenses (reducing lens costs from $10-30K t0 $1-3K) and the use of a ‘solid tank’ (which reduces noise, and the volume of refractively matched fluid from 1ltr to 10cc). The efficacy of DFOS was evaluated by direct comparison against commissioned scanners in our lab. Measured dose distributions from all systems were compared against the predicted dose distributions from a commissioned treatment planning system (TPS). Three treatment plans were investigated including a simple four-field box treatment, a multiple small field delivery, and a complex IMRT treatment. Dosimeters were imaged within 2h post irradiation, using consistent scanning techniques (360 projections acquired at 1 degree intervals, reconstruction at 2mm). DFOS efficacy was evaluated through inspection of dose line-profiles, and 2D and 3D dose and gamma maps. DFOS/TPS gamma pass rates with 3%/3mm dose difference/distance-to-agreement criteria ranged from 89.3% to 92.2%, compared to from 95.6% to 99.0% obtained with the commissioned system. The 3D gamma pass rate between the commissioned system and DFOS was 98.2%. The typical noise rates in DFOS reconstructions were up to 3%, compared to under 2% for the commissioned system. In conclusion, while the introduction of a solid tank proved advantageous with regards to cost and convenience, further work is required to improve the image quality and dose reconstruction accuracy of the new DFOS optical-CT system. PMID:27019460

  2. Optical-CT 3D Dosimetry Using Fresnel Lenses with Minimal Refractive-Index Matching Fluid.

    PubMed

    Bache, Steven; Malcolm, Javian; Adamovics, John; Oldham, Mark

    2016-01-01

    Telecentric optical computed tomography (optical-CT) is a state-of-the-art method for visualizing and quantifying 3-dimensional dose distributions in radiochromic dosimeters. In this work a prototype telecentric system (DFOS-Duke Fresnel Optical-CT Scanner) is evaluated which incorporates two substantial design changes: the use of Fresnel lenses (reducing lens costs from $10-30K t0 $1-3K) and the use of a 'solid tank' (which reduces noise, and the volume of refractively matched fluid from 1 ltr to 10 cc). The efficacy of DFOS was evaluated by direct comparison against commissioned scanners in our lab. Measured dose distributions from all systems were compared against the predicted dose distributions from a commissioned treatment planning system (TPS). Three treatment plans were investigated including a simple four-field box treatment, a multiple small field delivery, and a complex IMRT treatment. Dosimeters were imaged within 2 h post irradiation, using consistent scanning techniques (360 projections acquired at 1 degree intervals, reconstruction at 2mm). DFOS efficacy was evaluated through inspection of dose line-profiles, and 2D and 3D dose and gamma maps. DFOS/TPS gamma pass rates with 3%/3mm dose difference/distance-to-agreement criteria ranged from 89.3% to 92.2%, compared to from 95.6% to 99.0% obtained with the commissioned system. The 3D gamma pass rate between the commissioned system and DFOS was 98.2%. The typical noise rates in DFOS reconstructions were up to 3%, compared to under 2% for the commissioned system. In conclusion, while the introduction of a solid tank proved advantageous with regards to cost and convenience, further work is required to improve the image quality and dose reconstruction accuracy of the new DFOS optical-CT system.

  3. Reliability of a 3D surface laser scanner for orthodontic applications.

    PubMed

    Kusnoto, Budi; Evans, Carla A

    2002-10-01

    A device for recreating three-dimensional (3D) objects on a computer is the surface laser scanner. By triangulating distances between the reflecting laser beam and the scanned surface, the surface laser scanner can detect not only an object's length and width but also its depth. The scanner's ease of use has opened various possibilities in laboratory research and clinical investigation. We assessed the reliability of generating 3D object reconstructions using the Minolta Vivid700 3D surface laser scanner (Minolta USA, Ramsey, NJ). Accuracy and reproducibility were tested on a geometrical calibrated cylinder, a dental study model, and a plaster facial model. Tests were conducted at varying distances between the object and the scanner. It was found that (1) in the calibrated cylinder tests, spatial distance measurement was accurate to 0.5 mm (+/- 0.1 mm) in the vertical dimension and 0.3 mm (+/- 0.3 mm) in the horizontal dimension; (2) in the study model test, molar width was accurate to 0.2 mm (+/- 0.1 mm, P >.05), and palatal vault depth could be measured to 0.7 mm (+/- 0.2 mm, P > 0.05); and (3) for the facial model, an accuracy of 1.9 +/- 0.8 mm was obtained. The findings suggest that the surface laser scanner has great research potential because of its accuracy and ease of use. Treatment changes, growth, surgical simulations, and many other orthodontic applications can be approached 3-dimensionally with this device.

  4. Implementation of 3D prostrate ring-scanning mechanism for NIR diffuse optical imaging phantom validation

    NASA Astrophysics Data System (ADS)

    Yu, Jhao-Ming; Chen, Liang-Yu; Pan, Min-Cheng; Hsu, Ya-Fen; Pan, Min-Chun

    2015-03-01

    Diffuse optical imaging (DOI) providing functional information of tissues has drawn great attention for the last two decades. Near infrared (NIR) DOI systems composed of scanning bench, opt-electrical measurement module, system control, and data processing and image reconstruction schemes are developed for the screening and diagnosis of breast tumors. Mostly, the scanning bench belonging to fixed source-and-detector configuration limits computed image resolution to an extent. To cope with the issue, we propose, design and implement a 3D prostrate ring-scanning equipment for NIR DOI with flexible combinations of illumination and detection, and with the function of radial, circular and vertical movement without hard compression of breast tissue like the imaging system using or incorporating with X-ray mammographic bench. Especially, a rotation-sliding-and-moving mechanism was designed for the guidance of source- and detection-channel movement. Following the previous justification for synthesized image reconstruction, in the paper the validation using varied phantoms is further conducted and 3D image reconstruction for their absorption and scattering coefficients is illustrated through the computation of our in-house coded schemes. The source and detection NIR data are acquired to reconstruct the 3D images through the operation of scanning bench in the movement of vertical, radial and circular directions. Rather than the fixed configuration, the addressed screening/diagnosing equipment has the flexibility for optical-channel expansion with a compromise among construction cost, operation time, and spatial resolution of reconstructed μa and μs' images.

  5. Reconstruction and Visualization of Coordinated 3D Cell Migration Based on Optical Flow.

    PubMed

    Kappe, Christopher P; Schütz, Lucas; Gunther, Stefan; Hufnagel, Lars; Lemke, Steffen; Leitte, Heike

    2016-01-01

    Animal development is marked by the repeated reorganization of cells and cell populations, which ultimately determine form and shape of the growing organism. One of the central questions in developmental biology is to understand precisely how cells reorganize, as well as how and to what extent this reorganization is coordinated. While modern microscopes can record video data for every cell during animal development in 3D+t, analyzing these videos remains a major challenge: reconstruction of comprehensive cell tracks turned out to be very demanding especially with decreasing data quality and increasing cell densities. In this paper, we present an analysis pipeline for coordinated cellular motions in developing embryos based on the optical flow of a series of 3D images. We use numerical integration to reconstruct cellular long-term motions in the optical flow of the video, we take care of data validation, and we derive a LIC-based, dense flow visualization for the resulting pathlines. This approach allows us to handle low video quality such as noisy data or poorly separated cells, and it allows the biologists to get a comprehensive understanding of their data by capturing dynamic growth processes in stills. We validate our methods using three videos of growing fruit fly embryos.

  6. Optical lens-shift design for increasing spatial resolution of 3D ToF cameras

    NASA Astrophysics Data System (ADS)

    Lietz, Henrik; Hassan, M. Muneeb; Eberhardt, Jörg

    2017-02-01

    Sensor resolution of 3D time-of-flight (ToF) outdoor-capable cameras is strongly limited because of its large pixel dimensions. Computational imaging permits enhancement of the optical system's resolving power without changing physical sensor properties. Super-resolution (SR) algorithms superimpose several sub-pixel-shifted low-resolution (LR) images to overcome the system's limited spatial sampling rate. In this paper, we propose a novel opto-mechanical system to implement sub-pixel shifts by moving an optical lens. This method is more flexible in terms of implementing SR techniques than current sensor-shift approaches. In addition, we describe a SR observation model that has been optimized for the use of LR 3D ToF cameras. A state-of-the-art iteratively reweighted minimization algorithm executes the SR process. It is proven that our method achieves nearly the same resolution increase as if the pixel area would be halved physically. Resolution enhancement is measured objectively for amplitude images of a static object scene.

  7. Pico-projector-based optical sectioning microscopy for 3D chlorophyll fluorescence imaging of mesophyll cells

    NASA Astrophysics Data System (ADS)

    Chen, Szu-Yu; Hsu, Yu John; Yeh, Chia-Hua; Chen, S.-Wei; Chung, Chien-Han

    2015-03-01

    A pico-projector-based optical sectioning microscope (POSM) was constructed using a pico-projector to generate structured illumination patterns. A net rate of 5.8 × 106 pixel/s and sub-micron spatial resolution in three-dimensions (3D) were achieved. Based on the pico-projector’s flexibility in pattern generation, the characteristics of POSM with different modulation periods and at different imaging depths were measured and discussed. With the application of different modulation periods, 3D chlorophyll fluorescence imaging of mesophyll cells was carried out in freshly plucked leaves of four species without sectioning or staining. For each leaf, an average penetration depth of 120 μm was achieved. Increasing the modulation period along with the increment of imaging depth, optical sectioning images can be obtained with a compromise between the axial resolution and signal-to-noise ratio. After ∼30 min imaging on the same area, photodamage was hardly observed. Taking the advantages of high speed and low damages of POSM, the investigation of the dynamic fluorescence responses to temperature changes was performed under three different treatment temperatures. The three embedded blue, green and red light-emitting diode light sources were applied to observe the responses of the leaves with different wavelength excitation.

  8. SU-E-J-209: Verification of 3D Surface Registration Between Stereograms and CT Images

    SciTech Connect

    Han, T; Gifford, K; Smith, B; Salehpour, M

    2014-06-01

    Purpose: Stereography can provide a visualization of the skin surface for radiation therapy patients. The aim of this study was to verify the registration algorithm in a commercial image analysis software, 3dMDVultus, for the fusion of stereograms and CT images. Methods: CT and stereographic scans were acquired of a head phantom and a deformable phantom. CT images were imported in 3dMDVultus and the surface contours were generated by threshold segmentation. Stereograms were reconstructed in 3dMDVultus. The resulting surfaces were registered with Vultus algorithm and then exported to in-house registration software and compared with four algorithms: rigid, affine, non-rigid iterative closest point (ICP) and b-spline algorithm. RMS (root-mean-square residuals of the surface point distances) error between the registered CT and stereogram surfaces was calculated and analyzed. Results: For the head phantom, the maximum RMS error between registered CT surfaces to stereogram was 6.6 mm for Vultus algorithm, whereas the mean RMS error was 0.7 mm. For the deformable phantom, the maximum RMS error was 16.2 mm for Vultus algorithm, whereas the mean RMS error was 4.4 mm. Non-rigid ICP demonstrated the best registration accuracy, as the mean of RMS errors were both within 1 mm. Conclusion: The accuracy of registration algorithm in 3dMDVultus was verified and exceeded RMS of 2 mm for deformable cases. Non-rigid ICP and b-spline algorithms improve the registration accuracy for both phantoms, especially in deformable one. For those patients whose body habitus deforms during radiation therapy, more advanced nonrigid algorithms need to be used.

  9. Strain-Initialized Robust Bone Surface Detection in 3-D Ultrasound.

    PubMed

    Hussain, Mohammad Arafat; Hodgson, Antony J; Abugharbieh, Rafeef

    2017-03-01

    Three-dimensional ultrasound has been increasingly considered as a safe radiation-free alternative to radiation-based fluoroscopic imaging for surgical guidance during computer-assisted orthopedic interventions, but because ultrasound images contain significant artifacts, it is challenging to automatically extract bone surfaces from these images. We propose an effective way to extract 3-D bone surfaces using a surface growing approach that is seeded from 2-D bone contours. The initial 2-D bone contours are estimated from a combination of ultrasound strain images and envelope power images. Novel features of the proposed method include: (i) improvement of a previously reported 2-D strain imaging-based bone segmentation method by incorporation of a depth-dependent cumulative power of the envelope into the elastographic data; (ii) incorporation of an echo decorrelation measure-based weight to fuse the strain and envelope maps; (iii) use of local statistics of the bone surface candidate points to detect the presence of any bone discontinuity; and (iv) an extension of our 2-D bone contour into a 3-D bone surface by use of an effective surface growing approach. Our new method produced average improvements in the mean absolute error of 18% and 23%, respectively, on 2-D and 3-D experimental phantom data, compared with those of two state-of-the-art bone segmentation methods. Validation on 2-D and 3-D clinical in vivo data also reveals, respectively, an average improvement in the mean absolute fitting error of 55% and an 18-fold improvement in the computation time.

  10. The 3D scanner for measuring body surface area: a simplified calculation in the Chinese adult.

    PubMed

    Yu, Chi-Yuan; Lo, Yu-Hung; Chiou, Wen-Ko

    2003-05-01

    Three-dimensional (3D) surface anthropometry enables us to extend the study to 3D geometry and morphology of mainly external human body tissues. A model is presented for estimation of human body surface area (BSA), which is identical in form to the one proposed in 1916 by DuBois and DuBois is presented. The purpose of this study is to measure BSA, using 3D scanner, and to derive a simple BSA estimation formula for the Chinese adults. In as little as 12s, the Chang Gung Whole-Body Scanner (CGWBS) allows you to capture the shape of the entire human body. The total error in BSA measurement due to scanning measurement and software computational error is less than 1%. The 3D anthropometric measures in a healthy population (n=3951) were investigated, and the results were used to derive a BSA estimation formula. The results seem to be comparable to previous data that measured BSA using traditional methods. The BSA estimation model of this study also validated using 300 new measurements along with the formulae proposed in previous researches. The result suggests that our formula better fits our adults.

  11. Controlled implant/soft tissue interaction by nanoscale surface modifications of 3D porous titanium implants

    NASA Astrophysics Data System (ADS)

    Rieger, Elisabeth; Dupret-Bories, Agnès; Salou, Laetitia; Metz-Boutigue, Marie-Helene; Layrolle, Pierre; Debry, Christian; Lavalle, Philippe; Engin Vrana, Nihal

    2015-05-01

    Porous titanium implants are widely employed in the orthopaedics field to ensure good bone fixation. Recently, the use of porous titanium implants has also been investigated in artificial larynx development in a clinical setting. Such uses necessitate a better understanding of the interaction of soft tissues with porous titanium structures. Moreover, surface treatments of titanium have been generally evaluated in planar structures, while the porous titanium implants have complex 3 dimensional (3D) architectures. In this study, the determining factors for soft tissue integration of 3D porous titanium implants were investigated as a function of surface treatments via quantification of the interaction of serum proteins and cells with single titanium microbeads (300-500 μm in diameter). Samples were either acid etched or nanostructured by anodization. When the samples are used in 3D configuration (porous titanium discs of 2 mm thickness) in vivo (in subcutis of rats for 2 weeks), a better integration was observed for both anodized and acid etched samples compared to the non-treated implants. If the implants were also pre-treated with rat serum before implantation, the integration was further facilitated. In order to understand the underlying reasons for this effect, human fibroblast cell culture tests under several conditions (directly on beads, beads in suspension, beads encapsulated in gelatin hydrogels) were conducted to mimic the different interactions of cells with Ti implants in vivo. Physical characterization showed that surface treatments increased hydrophilicity, protein adsorption and roughness. Surface treatments also resulted in improved adsorption of serum albumin which in turn facilitated the adsorption of other proteins such as apolipoprotein as quantified by protein sequencing. The cellular response to the beads showed considerable difference with respect to the cell culture configuration. When the titanium microbeads were entrapped in cell

  12. Perception of 3D shape from homogeneous and nonhomogeneous surface textures

    NASA Astrophysics Data System (ADS)

    Li, Andrea; Zaidi, Qasim

    2004-06-01

    When a textured 3-dimensional surface is projected in perspective, the statistics of the texture in the image change with the shape of the surface. Most shape-from-texture models assume that these changes are due solely to the projection of non-fronto-parallel portions of the surface. This is true for developable surfaces, which are formed by bending or curving flat, textured sheets without tearing or stretching. However, for other surfaces such as those carved from solids or formed by stretched materials, the texture on the surface is generally not homogenous. If the perspective image is parsed into local Fourier spectra, we find that signature patterns of orientation flows occur at locations corresponding to specific 3-D shapes. These patterns occur generically for developable, carved and stretched surfaces and when they are visible, observers make veridical shape judgments. In contrast, frequency modulations vary systematically for different types of surfaces, and often lead to non-veridical percepts when they are caused by changes in slant (e.g. isotropically textured developable surfaces). Our results suggest that in the extraction of 3-D shape, the visual system can generically employ a limited number of neural mechanisms to extract the signature orientation flows from the image regardless of homogeneity.

  13. Segmentation of whole cells and cell nuclei from 3-D optical microscope images using dynamic programming.

    PubMed

    McCullough, D P; Gudla, P R; Harris, B S; Collins, J A; Meaburn, K J; Nakaya, M A; Yamaguchi, T P; Misteli, T; Lockett, S J

    2008-05-01

    Communications between cells in large part drive tissue development and function, as well as disease-related processes such as tumorigenesis. Understanding the mechanistic bases of these processes necessitates quantifying specific molecules in adjacent cells or cell nuclei of intact tissue. However, a major restriction on such analyses is the lack of an efficient method that correctly segments each object (cell or nucleus) from 3-D images of an intact tissue specimen. We report a highly reliable and accurate semi-automatic algorithmic method for segmenting fluorescence-labeled cells or nuclei from 3-D tissue images. Segmentation begins with semi-automatic, 2-D object delineation in a user-selected plane, using dynamic programming (DP) to locate the border with an accumulated intensity per unit length greater that any other possible border around the same object. Then the two surfaces of the object in planes above and below the selected plane are found using an algorithm that combines DP and combinatorial searching. Following segmentation, any perceived errors can be interactively corrected. Segmentation accuracy is not significantly affected by intermittent labeling of object surfaces, diffuse surfaces, or spurious signals away from surfaces. The unique strength of the segmentation method was demonstrated on a variety of biological tissue samples where all cells, including irregularly shaped cells, were accurately segmented based on visual inspection.

  14. Illustrating Surface Shape in Volume Data via Principal Direction-Driven 3D Line Integral Convolution

    NASA Technical Reports Server (NTRS)

    Interrante, Victoria

    1997-01-01

    The three-dimensional shape and relative depth of a smoothly curving layered transparent surface may be communicated particularly effectively when the surface is artistically enhanced with sparsely distributed opaque detail. This paper describes how the set of principal directions and principal curvatures specified by local geometric operators can be understood to define a natural 'flow' over the surface of an object, and can be used to guide the placement of the lines of a stroke texture that seeks to represent 3D shape information in a perceptually intuitive way. The driving application for this work is the visualization of layered isovalue surfaces in volume data, where the particular identity of an individual surface is not generally known a priori and observers will typically wish to view a variety of different level surfaces from the same distribution, superimposed over underlying opaque structures. By advecting an evenly distributed set of tiny opaque particles, and the empty space between them, via 3D line integral convolution through the vector field defined by the principal directions and principal curvatures of the level surfaces passing through each gridpoint of a 3D volume, it is possible to generate a single scan-converted solid stroke texture that may intuitively represent the essential shape information of any level surface in the volume. To generate longer strokes over more highly curved areas, where the directional information is both most stable and most relevant, and to simultaneously downplay the visual impact of directional information in the flatter regions, one may dynamically redefine the length of the filter kernel according to the magnitude of the maximum principal curvature of the level surface at the point around which it is applied.

  15. Evaluation Model for Pavement Surface Distress on 3d Point Clouds from Mobile Mapping System

    NASA Astrophysics Data System (ADS)

    Aoki, K.; Yamamoto, K.; Shimamura, H.

    2012-07-01

    This paper proposes a methodology to evaluate the pavement surface distress for maintenance planning of road pavement using 3D point clouds from Mobile Mapping System (MMS). The issue on maintenance planning of road pavement requires scheduled rehabilitation activities for damaged pavement sections to keep high level of services. The importance of this performance-based infrastructure asset management on actual inspection data is globally recognized. Inspection methodology of road pavement surface, a semi-automatic measurement system utilizing inspection vehicles for measuring surface deterioration indexes, such as cracking, rutting and IRI, have already been introduced and capable of continuously archiving the pavement performance data. However, any scheduled inspection using automatic measurement vehicle needs much cost according to the instruments' specification or inspection interval. Therefore, implementation of road maintenance work, especially for the local government, is difficult considering costeffectiveness. Based on this background, in this research, the methodologies for a simplified evaluation for pavement surface and assessment of damaged pavement section are proposed using 3D point clouds data to build urban 3D modelling. The simplified evaluation results of road surface were able to provide useful information for road administrator to find out the pavement section for a detailed examination and for an immediate repair work. In particular, the regularity of enumeration of 3D point clouds was evaluated using Chow-test and F-test model by extracting the section where the structural change of a coordinate value was remarkably achieved. Finally, the validity of the current methodology was investigated by conducting a case study dealing with the actual inspection data of the local roads.

  16. A Generalized Approach to the Modeling and Analysis of 3D Surface Morphology in Organisms

    PubMed Central

    Pappas, Janice L.; Miller, Daniel J.

    2013-01-01

    The surface geometry of an organism represents the boundary of its three-dimensional (3D) form and can be used as a proxy for the phenotype. A mathematical approach is presented that describes surface morphology using parametric 3D equations with variables expressed as x, y, z in terms of parameters u, v. Partial differentiation of variables with respect to parameters yields elements of the Jacobian representing tangent lines and planes of every point on the surface. Jacobian elements provide a compact size-free summary of the entire surface, and can be used as variables in principal components analysis to produce a morphospace. Mollusk and echinoid models are generated to demonstrate that whole organisms can be represented in a common morphospace, regardless of differences in size, geometry, and taxonomic affinity. Models can be used to simulate theoretical forms, novel morphologies, and patterns of phenotypic variation, and can also be empirically-based by designing them with reference to actual forms using reverse engineering principles. Although this study uses the Jacobian to summarize models, they can also be analyzed with 3D methods such as eigensurface, spherical harmonics, wavelet analysis, and geometric morphometrics. This general approach should prove useful for exploring broad questions regarding morphological evolution and variation. PMID:24204866

  17. Automatic 3D liver segmentation based on deep learning and globally optimized surface evolution.

    PubMed

    Hu, Peijun; Wu, Fa; Peng, Jialin; Liang, Ping; Kong, Dexing

    2016-12-21

    The detection and delineation of the liver from abdominal 3D computed tomography (CT) images are fundamental tasks in computer-assisted liver surgery planning. However, automatic and accurate segmentation, especially liver detection, remains challenging due to complex backgrounds, ambiguous boundaries, heterogeneous appearances and highly varied shapes of the liver. To address these difficulties, we propose an automatic segmentation framework based on 3D convolutional neural network (CNN) and globally optimized surface evolution. First, a deep 3D CNN is trained to learn a subject-specific probability map of the liver, which gives the initial surface and acts as a shape prior in the following segmentation step. Then, both global and local appearance information from the prior segmentation are adaptively incorporated into a segmentation model, which is globally optimized in a surface evolution way. The proposed method has been validated on 42 CT images from the public Sliver07 database and local hospitals. On the Sliver07 online testing set, the proposed method can achieve an overall score of [Formula: see text], yielding a mean Dice similarity coefficient of [Formula: see text], and an average symmetric surface distance of [Formula: see text] mm. The quantitative validations and comparisons show that the proposed method is accurate and effective for clinical application.

  18. Automatic 3D liver segmentation based on deep learning and globally optimized surface evolution

    NASA Astrophysics Data System (ADS)

    Hu, Peijun; Wu, Fa; Peng, Jialin; Liang, Ping; Kong, Dexing

    2016-12-01

    The detection and delineation of the liver from abdominal 3D computed tomography (CT) images are fundamental tasks in computer-assisted liver surgery planning. However, automatic and accurate segmentation, especially liver detection, remains challenging due to complex backgrounds, ambiguous boundaries, heterogeneous appearances and highly varied shapes of the liver. To address these difficulties, we propose an automatic segmentation framework based on 3D convolutional neural network (CNN) and globally optimized surface evolution. First, a deep 3D CNN is trained to learn a subject-specific probability map of the liver, which gives the initial surface and acts as a shape prior in the following segmentation step. Then, both global and local appearance information from the prior segmentation are adaptively incorporated into a segmentation model, which is globally optimized in a surface evolution way. The proposed method has been validated on 42 CT images from the public Sliver07 database and local hospitals. On the Sliver07 online testing set, the proposed method can achieve an overall score of 80.3+/- 4.5 , yielding a mean Dice similarity coefficient of 97.25+/- 0.65 % , and an average symmetric surface distance of 0.84+/- 0.25 mm. The quantitative validations and comparisons show that the proposed method is accurate and effective for clinical application.

  19. Development of portable 3D optical measuring system using structured light projection method

    NASA Astrophysics Data System (ADS)

    Aoki, Hiroshi

    2014-05-01

    Three-dimensional (3D) scanners are becoming increasingly common in many industries. However most of these scanning technologies have drawbacks for practical use due to size, weight, accessibility, and ease-of-use. Depending on the application, speed, flexibility and portability can often be deemed more important than accuracy. We have developed a solution to address this market requirement and overcome the aforementioned limitations. To counteract shortcomings such as heavy weight and large size, an optical sensor is used that consists of a laser projector, a camera system, and a multi-touch screen. Structured laser light is projected onto the measured object with a newly designed laser projector employing a single Micro Electro Mechanical Systems (MEMS) mirror. The optical system is optimized for the combination of a Laser Diode (LD), the MEMS mirror and the size of measurement area to secure the ideal contrast of structured light. Also, we developed a new calibration algorithm for this sensor with MEMS laser projector that uses an optical camera model for point cloud calculation. These technical advancements make the sensor compact, save power consumption, and reduce heat generation yet still allows for rapid calculation. Due to the principle of the measurement, structured light triangulation utilizing phase-shifting technology, resolution is improved. To meet requirements for practical applications, the optics, electronics, image processing, display and data management capabilities have been integrated into a single compact unit.

  20. Fiber based optical tweezers for simultaneous in situ force exertion and measurements in a 3D polyacrylamide gel compartment.

    PubMed

    Ti, Chaoyang; Thomas, Gawain M; Ren, Yundong; Zhang, Rui; Wen, Qi; Liu, Yuxiang

    2015-07-01

    Optical tweezers play an important role in biological applications. However, it is difficult for traditional optical tweezers based on objective lenses to work in a three-dimensional (3D) solid far away from the substrate. In this work, we develop a fiber based optical trapping system, namely inclined dual fiber optical tweezers, that can simultaneously apply and measure forces both in water and in a 3D polyacrylamide gel matrix. In addition, we demonstrate in situ, non-invasive characterization of local mechanical properties of polyacrylamide gel by measurements on an embedded bead. The fiber optical tweezers measurements agree well with those of atomic force microscopy (AFM). The inclined dual fiber optical tweezers provide a promising and versatile tool for cell mechanics study in 3D environments.

  1. Importance of a 3D forward modeling tool for surface wave analysis methods

    NASA Astrophysics Data System (ADS)

    Pageot, Damien; Le Feuvre, Mathieu; Donatienne, Leparoux; Philippe, Côte; Yann, Capdeville

    2016-04-01

    Since a few years, seismic surface waves analysis methods (SWM) have been widely developed and tested in the context of subsurface characterization and have demonstrated their effectiveness for sounding and monitoring purposes, e.g., high-resolution tomography of the principal geological units of California or real time monitoring of the Piton de la Fournaise volcano. Historically, these methods are mostly developed under the assumption of semi-infinite 1D layered medium without topography. The forward modeling is generally based on Thomson-Haskell matrix based modeling algorithm and the inversion is driven by Monte-Carlo sampling. Given their efficiency, SWM have been transfered to several scale of which civil engineering structures in order to, e.g., determine the so-called V s30 parameter or assess other critical constructional parameters in pavement engineering. However, at this scale, many structures may often exhibit 3D surface variations which drastically limit the efficiency of SWM application. Indeed, even in the case of an homogeneous structure, 3D geometry can bias the dispersion diagram of Rayleigh waves up to obtain discontinuous phase velocity curves which drastically impact the 1D mean velocity model obtained from dispersion inversion. Taking advantages of high-performance computing center accessibility and wave propagation modeling algorithm development, it is now possible to consider the use of a 3D elastic forward modeling algorithm instead of Thomson-Haskell method in the SWM inversion process. We use a parallelized 3D elastic modeling code based on the spectral element method which allows to obtain accurate synthetic data with very low numerical dispersion and a reasonable numerical cost. In this study, we choose dike embankments as an illustrative example. We first show that their longitudinal geometry may have a significant effect on dispersion diagrams of Rayleigh waves. Then, we demonstrate the necessity of 3D elastic modeling as a forward

  2. 3D precision measurements of meter sized surfaces using low cost illumination and camera techniques

    NASA Astrophysics Data System (ADS)

    Ekberg, Peter; Daemi, Bita; Mattsson, Lars

    2017-04-01

    Using dedicated stereo camera systems and structured light is a well-known method for measuring the 3D shape of large surfaces. However the problem is not trivial when high accuracy, in the range of few tens of microns, is needed. Many error sources need to be handled carefully in order to obtain high quality results. In this study, we present a measurement method based on low-cost camera and illumination solutions combined with high-precision image analysis and a new approach in camera calibration and 3D reconstruction. The setup consists of two ordinary digital cameras and a Gobo projector as a structured light source. A matrix of dots is projected onto the target area. The two cameras capture the images of the projected pattern on the object. The images are processed by advanced subpixel resolution algorithms prior to the application of the 3D reconstruction technique. The strength of the method lays in a different approach for calibration, 3D reconstruction, and high-precision image analysis algorithms. Using a 10 mm pitch pattern of the light dots, the method is capable of reconstructing the 3D shape of surfaces. The precision (1σ repeatability) in the measurements is  <10 µm over a volume of 60  ×  50  ×  10 cm3 at a hardware cost of ~2% of available advanced measurement techniques. The expanded uncertainty (95% confidence level) is estimated to be 83 µm, with the largest uncertainty contribution coming from the absolute length of the metal ruler used as reference.

  3. Towards a Noninvasive Intracranial Tumor Irradiation Using 3D Optical Imaging and Multimodal Data Registration

    PubMed Central

    Posada, R.; Daul, Ch.; Wolf, D.; Aletti, P.

    2007-01-01

    Conformal radiotherapy (CRT) results in high-precision tumor volume irradiation. In fractioned radiotherapy (FRT), lesions are irradiated in several sessions so that healthy neighbouring tissues are better preserved than when treatment is carried out in one fraction. In the case of intracranial tumors, classical methods of patient positioning in the irradiation machine coordinate system are invasive and only allow for CRT in one irradiation session. This contribution presents a noninvasive positioning method representing a first step towards the combination of CRT and FRT. The 3D data used for the positioning is point clouds spread over the patient's head (CT-data usually acquired during treatment) and points distributed over the patient's face which are acquired with a structured light sensor fixed in the therapy room. The geometrical transformation linking the coordinate systems of the diagnosis device (CT-modality) and the 3D sensor of the therapy room (visible light modality) is obtained by registering the surfaces represented by the two 3D point sets. The geometrical relationship between the coordinate systems of the 3D sensor and the irradiation machine is given by a calibration of the sensor position in the therapy room. The global transformation, computed with the two previous transformations, is sufficient to predict the tumor position in the irradiation machine coordinate system with only the corresponding position in the CT-coordinate system. Results obtained for a phantom show that the mean positioning error of tumors on the treatment machine isocentre is 0.4 mm. Tests performed with human data proved that the registration algorithm is accurate (0.1 mm mean distance between homologous points) and robust even for facial expression changes. PMID:18364992

  4. Scanning all-fiber-optic endomicroscopy system for 3D nonlinear optical imaging of biological tissues

    PubMed Central

    Wu, Yicong; Leng, Yuxin; Xi, Jiefeng; Li, Xingde

    2009-01-01

    An extremely compact all-fiber-optic scanning endomicroscopy system was developed for two-photon fluorescence (TPF) and second harmonic generation (SHG) imaging of biological samples. A conventional double-clad fiber (DCF) was employed in the endomicroscope for single-mode femtosecond pulse delivery, multimode nonlinear optical signals collection and fast two-dimensional scanning. A single photonic bandgap fiber (PBF) with negative group velocity dispersion at two-photon excitation wavelength (i.e. ~810 nm) was used for pulse prechirping in replacement of a bulky grating/lens-based pulse stretcher. The combined use of DCF and PBF in the endomicroscopy system made the endomicroscope basically a plug-and-play unit. The excellent imaging ability of the extremely compact all-fiber-optic nonlinear optical endomicroscopy system was demonstrated by SHG imaging of rat tail tendon and depth-resolved TPF imaging of epithelial tissues stained with acridine orange. The preliminary results suggested the promising potential of this extremely compact all-fiber-optic endomicroscopy system for real-time assessment of both epithelial and stromal structures in luminal organs. PMID:19434122

  5. Direct ambient noise tomography for 3-D near surface shear velocity structure: methodology and applications

    NASA Astrophysics Data System (ADS)

    Yao, H.; Fang, H.; Li, C.; Liu, Y.; Zhang, H.; van der Hilst, R. D.; Huang, Y. C.

    2014-12-01

    Ambient noise tomography has provided essential constraints on crustal and uppermost mantle shear velocity structure in global seismology. Recent studies demonstrate that high frequency (e.g., ~ 1 Hz) surface waves between receivers at short distances can be successfully retrieved from ambient noise cross-correlation and then be used for imaging near surface or shallow crustal shear velocity structures. This approach provides important information for strong ground motion prediction in seismically active area and overburden structure characterization in oil and gas fields. Here we propose a new tomographic method to invert all surface wave dispersion data for 3-D variations of shear wavespeed without the intermediate step of phase or group velocity maps.The method uses frequency-dependent propagation paths and a wavelet-based sparsity-constrained tomographic inversion. A fast marching method is used to compute, at each period, surface wave traveltimes and ray paths between sources and receivers. This avoids the assumption of great-circle propagation that is used in most surface wave tomographic studies, but which is not appropriate in complex media. The wavelet coefficients of the velocity model are estimated with an iteratively reweighted least squares (IRLS) algorithm, and upon iterations the surface wave ray paths and the data sensitivity matrix are updated from the newly obtained velocity model. We apply this new method to determine the 3-D near surface wavespeed variations in the Taipei basin of Taiwan, Hefei urban area and a shale and gas production field in China using the high-frequency interstation Rayleigh wave dispersion data extracted from ambient noisecross-correlation. The results reveal strong effects of off-great-circle propagation of high-frequency surface waves in these regions with above 30% shear wavespeed variations. The proposed approach is more efficient and robust than the traditional two-step surface wave tomography for imaging complex

  6. Robust Locally Weighted Regression For Ground Surface Extraction In Mobile Laser Scanning 3D Data

    NASA Astrophysics Data System (ADS)

    Nurunnabi, A.; West, G.; Belton, D.

    2013-10-01

    A new robust way for ground surface extraction from mobile laser scanning 3D point cloud data is proposed in this paper. Fitting polynomials along 2D/3D points is one of the well-known methods for filtering ground points, but it is evident that unorganized point clouds consist of multiple complex structures by nature so it is not suitable for fitting a parametric global model. The aim of this research is to develop and implement an algorithm to classify ground and non-ground points based on statistically robust locally weighted regression which fits a regression surface (line in 2D) by fitting without any predefined global functional relation among the variables of interest. Afterwards, the z (elevation)-values are robustly down weighted based on the residuals for the fitted points. The new set of down weighted z-values along with x (or y) values are used to get a new fit of the (lower) surface (line). The process of fitting and down-weighting continues until the difference between two consecutive fits is insignificant. Then the final fit represents the ground level of the given point cloud and the ground surface points can be extracted. The performance of the new method has been demonstrated through vehicle based mobile laser scanning 3D point cloud data from urban areas which include different problematic objects such as short walls, large buildings, electric poles, sign posts and cars. The method has potential in areas like building/construction footprint determination, 3D city modelling, corridor mapping and asset management.

  7. NDE of a 3-D surface crack using closely coupled probes for DCPD technique

    SciTech Connect

    Saka, M.; Abe, H.; Hirota, D.; Komura, I.

    1998-11-01

    A procedure of applying the d-c potential drop technique using the closely coupled probes to NDE of a 3-D surface crack is newly developed. The calibration equation for three sensors which differ in the distance between the probes is derived. Experiments validated the use of the calibration equation for the NDE of cracks. The method to use the three sensors properly based on the measuring sensitivity is shown.

  8. Using 3D Printers to Model Earth Surface Topography for Increased Student Understanding and Retention

    NASA Astrophysics Data System (ADS)

    Thesenga, David; Town, James

    2014-05-01

    In February 2000, the Space Shuttle Endeavour flew a specially modified radar system during an 11-day mission. The purpose of the multinational Shuttle Radar Topography Mission (SRTM) was to "obtain elevation data on a near-global scale to generate the most complete high-resolution digital topographic database of Earth" by using radar interferometry. The data and resulting products are now publicly available for download and give a view of the landscape removed of vegetation, buildings, and other structures. This new view of the Earth's topography allows us to see previously unmapped or poorly mapped regions of the Earth as well as providing a level of detail that was previously unknown using traditional topographic mapping techniques. Understanding and appreciating the geographic terrain is a complex but necessary requirement for middle school aged (11-14yo) students. Abstract in nature, topographic maps and other 2D renderings of the Earth's surface and features do not address the inherent spatial challenges of a concrete-learner and traditional methods of teaching can at times exacerbate the problem. Technological solutions such as 3D-imaging in programs like Google Earth are effective but lack the tactile realness that can make a large difference in learning comprehension and retention for these young students. First developed in the 1980's, 3D printers were not commercial reality until recently and the rapid rise in interest has driven down the cost. With the advent of sub US1500 3D printers, this technology has moved out of the high-end marketplace and into the local office supply store. Schools across the US and elsewhere in the world are adding 3D printers to their technological workspaces and students have begun rapid-prototyping and manufacturing a variety of projects. This project attempted to streamline the process of transforming SRTM data from a GeoTIFF format by way of Python code. The resulting data was then inputted into a CAD-based program for

  9. A Hierarchical Building Segmentation in Digital Surface Models for 3D Reconstruction

    PubMed Central

    Yan, Yiming; Gao, Fengjiao; Deng, Shupei; Su, Nan

    2017-01-01

    In this study, a hierarchical method for segmenting buildings in a digital surface model (DSM), which is used in a novel framework for 3D reconstruction, is proposed. Most 3D reconstructions of buildings are model-based. However, the limitations of these methods are overreliance on completeness of the offline-constructed models of buildings, and the completeness is not easily guaranteed since in modern cities buildings can be of a variety of types. Therefore, a model-free framework using high precision DSM and texture-images buildings was introduced. There are two key problems with this framework. The first one is how to accurately extract the buildings from the DSM. Most segmentation methods are limited by either the terrain factors or the difficult choice of parameter-settings. A level-set method are employed to roughly find the building regions in the DSM, and then a recently proposed ‘occlusions of random textures model’ are used to enhance the local segmentation of the buildings. The second problem is how to generate the facades of buildings. Synergizing with the corresponding texture-images, we propose a roof-contour guided interpolation of building facades. The 3D reconstruction results achieved by airborne-like images and satellites are compared. Experiments show that the segmentation method has good performance, and 3D reconstruction is easily performed by our framework, and better visualization results can be obtained by airborne-like images, which can be further replaced by UAV images. PMID:28125018

  10. 3D surface imaging of the human female torso in upright to supine positions.

    PubMed

    Reece, Gregory P; Merchant, Fatima; Andon, Johnny; Khatam, Hamed; Ravi-Chandar, K; Weston, June; Fingeret, Michelle C; Lane, Chris; Duncan, Kelly; Markey, Mia K

    2015-04-01

    Three-dimensional (3D) surface imaging of breasts is usually done with the patient in an upright position, which does not permit comparison of changes in breast morphology with changes in position of the torso. In theory, these limitations may be eliminated if the 3D camera system could remain fixed relative to the woman's torso as she is tilted from 0 to 90°. We mounted a 3dMDtorso imaging system onto a bariatric tilt table to image breasts at different tilt angles. The images were validated using a rigid plastic mannequin and the metrics compared to breast metrics obtained from five subjects with diverse morphology. The differences between distances between the same fiducial marks differed between the supine and upright positions by less than 1% for the mannequin, whereas the differences for distances between the same fiducial marks on the breasts of the five subjects differed significantly and could be correlated with body mass index and brassiere cup size for each position change. We show that a tilt table-3D imaging system can be used to determine quantitative changes in the morphology of ptotic breasts when the subject is tilted to various angles.

  11. 3D surface scan of biological samples with a Push-broom Imaging Spectrometer

    NASA Astrophysics Data System (ADS)

    Yao, Haibo; Kincaid, Russell; Hruska, Zuzana; Brown, Robert L.; Bhatnagar, Deepak; Cleveland, Thomas E.

    2013-08-01

    The food industry is always on the lookout for sensing technologies for rapid and nondestructive inspection of food products. Hyperspectral imaging technology integrates both imaging and spectroscopy into unique imaging sensors. Its application for food safety and quality inspection has made significant progress in recent years. Specifically, hyperspectral imaging has shown its potential for surface contamination detection in many food related applications. Most existing hyperspectral imaging systems use pushbroom scanning which is generally used for flat surface inspection. In some applications it is desirable to be able to acquire hyperspectral images on circular objects such as corn ears, apples, and cucumbers. Past research describes inspection systems that examine all surfaces of individual objects. Most of these systems did not employ hyperspectral imaging. These systems typically utilized a roller to rotate an object, such as an apple. During apple rotation, the camera took multiple images in order to cover the complete surface of the apple. The acquired image data lacked the spectral component present in a hyperspectral image. This paper discusses the development of a hyperspectral imaging system for a 3-D surface scan of biological samples. The new instrument is based on a pushbroom hyperspectral line scanner using a rotational stage to turn the sample. The system is suitable for whole surface hyperspectral imaging of circular objects. In addition to its value to the food industry, the system could be useful for other applications involving 3-D surface inspection.

  12. Assessment of Ulcer Wounds Size Using 3D Skin Surface Imaging

    NASA Astrophysics Data System (ADS)

    Hani, Ahmad Fadzil M.; Eltegani, Nejood M.; Hussein, Suraiya H.; Jamil, Adawiyah; Gill, Priya

    In this work 3D surface scans of wounds are used to obtain several measurement including wound top area, true surface area (rue area), depth, and volume for the purpose of assessing the progress of ulcer wounds throughout treatment. KONICA MINOLTA 910 laser scanner is used to obtain the surface scans. The algorithm for estimating top area and true surface area from surface scan can reduce the inaccuracy that might result when using manual method. Two methods for solid construction and volume computation were considered; namely mid-point projection and convex hull approximation (Delaunay tetrahedralization). The performance of convex hull approximation method for volume estimation is improved by performing surface subdivision prior to the approximation. The performance of these algorithms on different patterns of simulated wound models is presented. Furthermore the algorithms are tested in two molded wounds printed using rapid prototyping (RP) technique.

  13. Modeling of 3d Space-time Surface of Potential Fields and Hydrogeologic Modeling of Nuclear Waste Disposal Sites

    NASA Astrophysics Data System (ADS)

    Shestopalov, V.; Bondarenko, Y.; Zayonts, I.; Rudenko, Y.

    Introduction After the Chernobyl Nuclear Power Plant (CNPP) disaster (04.26.1986) a huge amount (over 2000 sq. km) of nuclear wastes appeared within so-called "Cher- nobyl Exclusion Zone" (CEZ). At present there are not enough storage facilities in the Ukraine for safe disposal of nuclear wastes and hazardous chemical wastes. The urgent problem now is safe isolation of these dangerous wastes. According to the developed state program of radioactive waste management, the construction of a na- tional storage facility of nuclear wastes is planned. It is also possible to create regional storage facilities for hazardous chemical wastes. The region of our exploration cov- ers the eastern part of the Korosten Plutone and its slope, reaching the CNPP. 3D Space-Time Surface Imaging of Geophysical Fields. There are only three direct meth- ods of stress field reconstruction in present practice, namely the field investigations based on the large-scale fracturing tests, petrotectonic and optical polarization meth- ods. Unfortunately, all these methods are extremely laborious and need the regular field tests, which is difficult to conduct in the areas of anisotropic rock outcrops. A compilation of magnetic and gravity data covering the CNPP area was carried out as a prelude to an interpretation study. More than thirty map products were generated from magnetic, gravity and geodesy data to prepare the 3D Space-Time Surface Images (3D STSI). Multi-layer topography and geophysic surfaces included: total magnetic intensity, isostatically-corrected Bouguer gravity, aspect and slope, first and second derivatives, vertical and horizontal curvature, histogram characteristics and space cor- relation coefficients between the gradient fields. Many maps shows the first and sec- ond derivatives of the potential fields, with the results of lineament (edge) structure detection superimposed. The lineament or edges of the potential fields are located from maximal gradient in many directions

  14. A novel lithography process for 3D (three-dimensional) interconnect using an optical direct-writing exposure system

    NASA Astrophysics Data System (ADS)

    Azuma, T.; Sekiguchi, M.; Matsuo, M.; Kawasaki, A.; Hagiwara, K.; Matsui, H.; Kawamura, N.; Kishimoto, K.; Nakamura, A.; Washio, Y.

    2010-03-01

    A novel lithography process for 3D (Three-dimensional) interconnect was developed using an optical direct-writing exposure tool. A reflective IR (Infra-red) alignment system allows a direct detection of alignment marks both on front-side and back-side of wafer, and consequently allows feasible micro-fabrication for 3D interconnect using the reversed wafer. A combination of the optical direct-writing exposure tool of Dainippon Screen MFG. Co., Ltd. with the reflective IR alignment system and a high aspect chemically amplified resist of Tokyo Ohka Kogyo Co., Ltd. provides the lithography process exclusively for 12-inch wafer level 3D interconnect.

  15. Numerical Simulation of Two-grid Ion Optics Using a 3D Code

    NASA Technical Reports Server (NTRS)

    Anderson, John R.; Katz, Ira; Goebel, Dan

    2004-01-01

    A three-dimensional ion optics code has been developed under NASA's Project Prometheus to model two grid ion optics systems. The code computes the flow of positive ions from the discharge chamber through the ion optics and into the beam downstream of the thruster. The rate at which beam ions interact with background neutral gas to form charge exchange ions is also computed. Charge exchange ion trajectories are computed to determine where they strike the ion optics grid surfaces and to determine the extent of sputter erosion they cause. The code has been used to compute predictions of the erosion pattern and wear rate on the NSTAR ion optics system; the code predicts the shape of the eroded pattern but overestimates the initial wear rate by about 50%. An example of use of the code to estimate the NEXIS thruster accelerator grid life is also presented.

  16. 3D optical coherence tomography image registration for guiding cochlear implant insertion

    NASA Astrophysics Data System (ADS)

    Cheon, Gyeong-Woo; Jeong, Hyun-Woo; Chalasani, Preetham; Chien, Wade W.; Iordachita, Iulian; Taylor, Russell; Niparko, John; Kang, Jin U.

    2014-03-01

    In cochlear implant surgery, an electrode array is inserted into the cochlear canal to restore hearing to a person who is profoundly deaf or significantly hearing impaired. One critical part of the procedure is the insertion of the electrode array, which looks like a thin wire, into the cochlear canal. Although X-ray or computed tomography (CT) could be used as a reference to evaluate the pathway of the whole electrode array, there is no way to depict the intra-cochlear canal and basal turn intra-operatively to help guide insertion of the electrode array. Optical coherent tomography (OCT) is a highly effective way of visualizing internal structures of cochlea. Swept source OCT (SSOCT) having center wavelength of 1.3 micron and 2D Galvonometer mirrors was used to achieve 7-mm depth 3-D imaging. Graphics processing unit (GPU), OpenGL, C++ and C# were integrated for real-time volumetric rendering simultaneously. The 3D volume images taken by the OCT system were assembled and registered which could be used to guide a cochlear implant. We performed a feasibility study using both dry and wet temporal bones and the result is presented.

  17. Holographic microscopy and microfluidics platform for measuring wall stress and 3D flow over surfaces textured by micro-pillars

    PubMed Central

    Bocanegra Evans, Humberto; Gorumlu, Serdar; Aksak, Burak; Castillo, Luciano; Sheng, Jian

    2016-01-01

    Understanding how fluid flow interacts with micro-textured surfaces is crucial for a broad range of key biological processes and engineering applications including particle dispersion, pathogenic infections, and drag manipulation by surface topology. We use high-speed digital holographic microscopy (DHM) in combination with a correlation based de-noising algorithm to overcome the optical interference generated by surface roughness and to capture a large number of 3D particle trajectories in a microfluidic channel with one surface patterned with micropillars. It allows us to obtain a 3D ensembled velocity field with an uncertainty of 0.06% and 2D wall shear stress distribution at the resolution of ~65 μPa. Contrary to laminar flow in most microfluidics, we find that the flow is three-dimensional and complex for the textured microchannel. While the micropillars affect the velocity flow field locally, their presence is felt globally in terms of wall shear stresses at the channel walls. These findings imply that micro-scale mixing and wall stress sensing/manipulation can be achieved through hydro-dynamically smooth but topologically rough micropillars. PMID:27353632

  18. [Investigation of the surface layer of 3D-matrices for tissue engineering].

    PubMed

    Chernonosova, V S; Kvon, R I; Kiseleva, E V; Stepanova, A O; Laktionov, P P

    2017-01-01

    Electrospinning is a convenient and promising manufacturing method a variety of materials for tissue engineering. 3D matrices fabricated by electrospinning from solutions of polycaprolactone with human serum albumin or gelatin in 1,1,1,3,3,3-hexafluoroisopropanol were studied. The microstructure of the 3D matrices and surface of the fibers were investigated using scanning electron microscopy. Protein distribution in the surface layer was studied by modification of protein amino groups with N-(2-hydroxyethyl)phenazine and X-ray photoelectron spectroscopy. It was shown, that concentration of the proteins in the surface layer of fibers exceeded their concentration in the initial electrospun solution up to 12 times and the surface layer was enriched in the protein inversely to the concentration of the protein in solution. The minor part of the proteins was released from fibers during first 30-60 min after swelling in water. Treatment of matrices with proteinase K hydrolyzed about 1/3 of the surface exposed human serum albumin. Thus, both methods can be used to study the surface content of the materials produced by electrospinning from blends of synthetic and natural polymers, however X-ray photoelectron spectroscopy appears to be more convenient and informative.

  19. Defining an optimal surface chemistry for pluripotent stem cell culture in 2D and 3D

    NASA Astrophysics Data System (ADS)

    Zonca, Michael R., Jr.

    Surface chemistry is critical for growing pluripotent stem cells in an undifferentiated state. There is great potential to engineer the surface chemistry at the nanoscale level to regulate stem cell adhesion. However, the challenge is to identify the optimal surface chemistry of the substrata for ES cell attachment and maintenance. Using a high-throughput polymerization and screening platform, a chemically defined, synthetic polymer grafted coating that supports strong attachment and high expansion capacity of pluripotent stem cells has been discovered using mouse embryonic stem (ES) cells as a model system. This optimal substrate, N-[3-(Dimethylamino)propyl] methacrylamide (DMAPMA) that is grafted on 2D synthetic poly(ether sulfone) (PES) membrane, sustains the self-renewal of ES cells (up to 7 passages). DMAPMA supports cell attachment of ES cells through integrin beta1 in a RGD-independent manner and is similar to another recently reported polymer surface. Next, DMAPMA has been able to be transferred to 3D by grafting to synthetic, polymeric, PES fibrous matrices through both photo-induced and plasma-induced polymerization. These 3D modified fibers exhibited higher cell proliferation and greater expression of pluripotency markers of mouse ES cells than 2D PES membranes. Our results indicated that desirable surfaces in 2D can be scaled to 3D and that both surface chemistry and structural dimension strongly influence the growth and differentiation of pluripotent stem cells. Lastly, the feasibility of incorporating DMAPMA into a widely used natural polymer, alginate, has been tested. Novel adhesive alginate hydrogels have been successfully synthesized by either direct polymerization of DMAPMA and methacrylic acid blended with alginate, or photo-induced DMAPMA polymerization on alginate nanofibrous hydrogels. In particular, DMAPMA-coated alginate hydrogels support strong ES cell attachment, exhibiting a concentration dependency of DMAPMA. This research provides a

  20. Evaluation of precision and accuracy assessment of different 3-D surface imaging systems for biomedical purposes.

    PubMed

    Eder, Maximilian; Brockmann, Gernot; Zimmermann, Alexander; Papadopoulos, Moschos A; Schwenzer-Zimmerer, Katja; Zeilhofer, Hans Florian; Sader, Robert; Papadopulos, Nikolaos A; Kovacs, Laszlo

    2013-04-01

    Three-dimensional (3-D) surface imaging has gained clinical acceptance, especially in the field of cranio-maxillo-facial and plastic, reconstructive, and aesthetic surgery. Six scanners based on different scanning principles (Minolta Vivid 910®, Polhemus FastSCAN™, GFM PRIMOS®, GFM TopoCAM®, Steinbichler Comet® Vario Zoom 250, 3dMD DSP 400®) were used to measure five sheep skulls of different sizes. In three areas with varying anatomical complexity (areas, 1 = high; 2 = moderate; 3 = low), 56 distances between 20 landmarks are defined on each skull. Manual measurement (MM), coordinate machine measurements (CMM) and computer tomography (CT) measurements were used to define a reference method for further precision and accuracy evaluation of different 3-D scanning systems. MM showed high correlation to CMM and CT measurements (both r = 0.987; p < 0.001) and served as the reference method. TopoCAM®, Comet® and Vivid 910® showed highest measurement precision over all areas of complexity; Vivid 910®, the Comet® and the DSP 400® demonstrated highest accuracy over all areas with Vivid 910® being most accurate in areas 1 and 3, and the DSP 400® most accurate in area 2. In accordance to the measured distance length, most 3-D devices present higher measurement precision and accuracy for large distances and lower degrees of precision and accuracy for short distances. In general, higher degrees of complexity are associated with lower 3-D assessment accuracy, suggesting that for optimal results, different types of scanners should be applied to specific clinical applications and medical problems according to their special construction designs and characteristics.

  1. Optical security and anti-counterfeiting using 3D screen printing

    NASA Astrophysics Data System (ADS)

    Wu, W. H.; Yang, W. K.; Cheng, S. H.; Kuo, M. K.; Lee, H. W.; Chang, C. C.; Jeng, G. R.; Liu, C. P.

    2007-04-01

    This work presents a novel method for optical decrypted key production by screen printing technology. The key is mainly used to decrypt encoded information hidden inside documents containing Moire patterns and integral photographic 3D auto-stereoscopic images as a second-line security file. The proposed method can also be applied as an anti-counterfeiting measure in artistic screening. Decryption is performed by matching the correct angle between the decoding key and the document with a text or a simple geometric pattern. This study presents the theoretical analysis and experimental results of the decoded key production by the best parameter combination of Moire pattern size and screen printing elements. Experimental results reveal that the proposed method can be applied in anti-counterfeit document design for the fast and low-cost production of decryption key.

  2. 3D optical phase reconstruction within PMMA samples using a spectral OCT system

    NASA Astrophysics Data System (ADS)

    Briones-R., Manuel d. J.; De La Torre-Ibarra, Manuel H.; Mendoza Santoyo, Fernando

    2015-08-01

    The optical coherence tomography (OCT) technique has proved to be a useful method in biomedical areas such as ophthalmology, dentistry, dermatology, among many others. In all these applications the main target is to reconstruct the internal structure of the samples from which the physician's expertise may recognize and diagnose the existence of a disease. Nowadays OCT has been applied one step further and is used to study the mechanics of some particular type of materials, where the resulting information involves more than just their internal structure and the measurement of parameters such as displacements, stress and strain. Here we report on a spectral OCT system used to image the internal 3D microstructure and displacement maps from a PMMA (Poly-methyl-methacrylate) sample, subjected to a deformation by a controlled three point bending and tilting. The internal mechanical response of the polymer is shown as consecutive 2D images.

  3. Surface states in a 3D topological insulator: The role of hexagonal warping and curvature

    SciTech Connect

    Repin, E. V.; Burmistrov, I. S.

    2015-09-15

    We explore a combined effect of hexagonal warping and a finite effective mass on both the tunneling density of electronic surface states and the structure of Landau levels of 3D topological insulators. We find the increasing warping to transform the square-root van Hove singularity into a logarithmic one. For moderate warping, an additional logarithmic singularity and a jump in the tunneling density of surface states appear. By combining the perturbation theory and the WKB approximation, we calculate the Landau levels in the presence of hexagonal warping. We predict that due to the degeneracy removal, the evolution of Landau levels in the magnetic field is drastically modified.

  4. Intra-retinal layer segmentation of 3D optical coherence tomography using coarse grained diffusion map.

    PubMed

    Kafieh, Raheleh; Rabbani, Hossein; Abramoff, Michael D; Sonka, Milan

    2013-12-01

    Optical coherence tomography (OCT) is a powerful and noninvasive method for retinal imaging. In this paper, we introduce a fast segmentation method based on a new variant of spectral graph theory named diffusion maps. The research is performed on spectral domain (SD) OCT images depicting macular and optic nerve head appearance. The presented approach does not require edge-based image information in localizing most of boundaries and relies on regional image texture. Consequently, the proposed method demonstrates robustness in situations of low image contrast or poor layer-to-layer image gradients. Diffusion mapping applied to 2D and 3D OCT datasets is composed of two steps, one for partitioning the data into important and less important sections, and another one for localization of internal layers. In the first step, the pixels/voxels are grouped in rectangular/cubic sets to form a graph node. The weights of the graph are calculated based on geometric distances between pixels/voxels and differences of their mean intensity. The first diffusion map clusters the data into three parts, the second of which is the area of interest. The other two sections are eliminated from the remaining calculations. In the second step, the remaining area is subjected to another diffusion map assessment and the internal layers are localized based on their textural similarities. The proposed method was tested on 23 datasets from two patient groups (glaucoma and normals). The mean unsigned border positioning errors (mean ± SD) was 8.52 ± 3.13 and 7.56 ± 2.95 μm for the 2D and 3D methods, respectively.

  5. Automatic feature detection for 3D surface reconstruction from HDTV endoscopic videos

    NASA Astrophysics Data System (ADS)

    Groch, Anja; Baumhauer, Matthias; Meinzer, Hans-Peter; Maier-Hein, Lena

    2010-02-01

    A growing number of applications in the field of computer-assisted laparoscopic interventions depend on accurate and fast 3D surface acquisition. The most commonly applied methods for 3D reconstruction of organ surfaces from 2D endoscopic images involve establishment of correspondences in image pairs to allow for computation of 3D point coordinates via triangulation. The popular feature-based approach for correspondence search applies a feature descriptor to compute high-dimensional feature vectors describing the characteristics of selected image points. Correspondences are established between image points with similar feature vectors. In a previous study, the performance of a large set of state-of-the art descriptors for the use in minimally invasive surgery was assessed. However, standard Phase Alternating Line (PAL) endoscopic images were utilized for this purpose. In this paper, we apply some of the best performing feature descriptors to in-vivo PAL endoscopic images as well as to High Definition Television (HDTV) endoscopic images of the same scene and show that the quality of the correspondences can be increased significantly when using high resolution images.

  6. Volatile transport on inhomogeneous surfaces: II. Numerical calculations (VT3D)

    NASA Astrophysics Data System (ADS)

    Young, Leslie A.

    2017-03-01

    Several distant icy worlds have atmospheres that are in vapor-pressure equilibrium with their surface volatiles, including Pluto, Triton, and, probably, several large KBOs near perihelion. Studies of the volatile and thermal evolution of these have been limited by computational speed, especially for models that treat surfaces that vary with both latitude and longitude. In order to expedite such work, I present a new numerical model for the seasonal behavior of Pluto and Triton which (i) uses initial conditions that improve convergence, (ii) uses an expedient method for handling the transition between global and non-global atmospheres, (iii) includes local conservation of energy and global conservation of mass to partition energy between heating, conduction, and sublimation or condensation, (iv) uses time-stepping algorithms that ensure stability while allowing larger timesteps, and (v) can include longitudinal variability. This model, called VT3D, has been used in Young (2012a, 2012b), Young (2013), Olkin et al. (2015), Young and McKinnon (2013), and French et al. (2015). Many elements of VT3D can be used independently. For example, VT3D can also be used to speed up thermophysical models (Spencer et al., 1989) for bodies without volatiles. Code implementation is included in the supplemental materials and is available from the author.

  7. Magellan 3D perspective of Venus surface in western Eistla Regio

    NASA Technical Reports Server (NTRS)

    1991-01-01

    Magellan synthetic aperture radar data was used to create this three- dimensional (3D) perspective view of Venus' western Eistla Regio. This viewpoint is located at 1,310 kilometers (812 miles) southwest of Gula Mons at an elevation of 0.178 kilometers (0.48 miles). The view is of the northeast with Gula Mons appearing on the horizon. Gula Mons, a 3 kilometer (1.86 mile) high volcano, is located at approximately 22 degrees north latitude, 359 degrees east longitude. The impact crater Cunitz, named for the astronomer and mathematician Maria Cunitz, is visible in the center of the image. The crater is 48.5 kilometers (30 miles) in diameter and is 215 kilometers (133 miles) from the viewer's position. Magellan synthetic aperture radar data is combined with radar altimetry to develop a 3D map of the surface. Rays cast in a computer intersect the surface to create a 3D view. Simulated color and a digital elevation map developed by the United States (U.S.) Geological Survey is used to enhanc

  8. SU-E-J-128: 3D Surface Reconstruction of a Patient Using Epipolar Geometry

    SciTech Connect

    Kotoku, J; Nakabayashi, S; Kumagai, S; Ishibashi, T; Kobayashi, T; Haga, A; Saotome, N; Arai, N

    2014-06-01

    Purpose: To obtain a 3D surface data of a patient in a non-invasive way can substantially reduce the effort for the registration of patient in radiation therapy. To achieve this goal, we introduced the multiple view stereo technique, which is known to be used in a 'photo tourism' on the internet. Methods: 70 Images were taken with a digital single-lens reflex camera from different angles and positions. The camera positions and angles were inferred later in the reconstruction step. A sparse 3D reconstruction model was locating by SIFT features, which is robust for rotation and shift variance, in each image. We then found a set of correspondences between pairs of images by computing the fundamental matrix using the eight-point algorithm with RANSAC. After the pair matching, we optimized the parameter including camera positions to minimize the reprojection error by use of bundle adjustment technique (non-linear optimization). As a final step, we performed dense reconstruction and associate a color with each point using the library of PMVS. Results: Surface data were reconstructed well by visual inspection. The human skin is reconstructed well, althogh the reconstruction was time-consuming for direct use in daily clinical practice. Conclusion: 3D reconstruction using multi view stereo geometry is a promising tool for reducing the effort of patient setup. This work was supported by JSPS KAKENHI(25861128)

  9. Elastic shape analysis of cylindrical surfaces for 3D/2D registration in endometrial tissue characterization.

    PubMed

    Samir, Chafik; Kurtek, Sebastian; Srivastava, Anuj; Canis, Michel

    2014-05-01

    We study the problem of joint registration and deformation analysis of endometrial tissue using 3D magnetic resonance imaging (MRI) and 2D trans-vaginal ultrasound (TVUS) measurements. In addition to the different imaging techniques involved in the two modalities, this problem is complicated due to: 1) different patient pose during MRI and TVUS observations, 2) the 3D nature of MRI and 2D nature of TVUS measurements, 3) the unknown intersecting plane for TVUS in MRI volume, and 4) the potential deformation of endometrial tissue during TVUS measurement process. Focusing on the shape of the tissue, we use expert manual segmentation of its boundaries in the two modalities and apply, with modification, recent developments in shape analysis of parametric surfaces to this problem. First, we extend the 2D TVUS curves to generalized cylindrical surfaces through replication, and then we compare them with MRI surfaces using elastic shape analysis. This shape analysis provides a simultaneous registration (optimal reparameterization) and deformation (geodesic) between any two parametrized surfaces. Specifically, it provides optimal curves on MRI surfaces that match with the original TVUS curves. This framework results in an accurate quantification and localization of the deformable endometrial cells for radiologists, and growth characterization for gynecologists and obstetricians. We present experimental results using semi-synthetic data and real data from patients to illustrate these ideas.

  10. Maximum likelihood estimation of parameterized 3-D surfaces using a moving camera

    NASA Technical Reports Server (NTRS)

    Hung, Y.; Cernuschi-Frias, B.; Cooper, D. B.

    1987-01-01

    A new approach is introduced to estimating object surfaces in three-dimensional space from a sequence of images. A surface of interest here is modeled as a 3-D function known up to the values of a few parameters. The approach will work with any parameterization. However, in work to date researchers have modeled objects as patches of spheres, cylinders, and planes - primitive objects. These primitive surfaces are special cases of 3-D quadric surfaces. Primitive surface estimation is treated as the general problem of maximum likelihood parameter estimation based on two or more functionally related data sets. In the present case, these data sets constitute a sequence of images taken at different locations and orientations. A simple geometric explanation is given for the estimation algorithm. Though various techniques can be used to implement this nonlinear estimation, researches discuss the use of gradient descent. Experiments are run and discussed for the case of a sphere of unknown location. These experiments graphically illustrate the various advantages of using as many images as possible in the estimation and of distributing camera positions from first to last over as large a baseline as possible. Researchers introduce the use of asymptotic Bayesian approximations in order to summarize the useful information in a sequence of images, thereby drastically reducing both the storage and amount of processing required.

  11. Building 3D aerial image in photoresist with reconstructed mask image acquired with optical microscope

    NASA Astrophysics Data System (ADS)

    Chou, C. S.; Tang, Y. P.; Chu, F. S.; Huang, W. C.; Liu, R. G.; Gau, T. S.

    2012-03-01

    Calibration of mask images on wafer becomes more important as features shrink. Two major types of metrology have been commonly adopted. One is to measure the mask image with scanning electron microscope (SEM) to obtain the contours on mask and then simulate the wafer image with optical simulator. The other is to use an optical imaging tool Aerial Image Measurement System (AIMSTM) to emulate the image on wafer. However, the SEM method is indirect. It just gathers planar contours on a mask with no consideration of optical characteristics such as 3D topography structures. Hence, the image on wafer is not predicted precisely. Though the AIMSTM method can be used to directly measure the intensity at the near field of a mask but the image measured this way is not quite the same as that on the wafer due to reflections and refractions in the films on wafer. Here, a new approach is proposed to emulate the image on wafer more precisely. The behavior of plane waves with different oblique angles is well known inside and between planar film stacks. In an optical microscope imaging system, plane waves can be extracted from the pupil plane with a coherent point source of illumination. Once plane waves with a specific coherent illumination are analyzed, the partially coherent component of waves could be reconstructed with a proper transfer function, which includes lens aberration, polarization, reflection and refraction in films. It is a new method that we can transfer near light field of a mask into an image on wafer without the disadvantages of indirect SEM measurement such as neglecting effects of mask topography, reflections and refractions in the wafer film stacks. Furthermore, with this precise latent image, a separated resist model also becomes more achievable.

  12. Oscillating optical tweezer-based 3-D confocal microrheometer for investigating the intracellular micromechanics and structures

    NASA Astrophysics Data System (ADS)

    Ou-Yang, H. D.; Rickter, E. A.; Pu, C.; Latinovic, O.; Kumar, A.; Mengistu, M.; Lowe-Krentz, L.; Chien, S.

    2005-08-01

    Mechanical properties of living biological cells are important for cells to maintain their shapes, support mechanical stresses and move through tissue matrix. The use of optical tweezers to measure micromechanical properties of cells has recently made significant progresses. This paper presents a new approach, the oscillating optical tweezer cytorheometer (OOTC), which takes advantage of the coherent detection of harmonically modulated particle motions by a lock-in amplifier to increase sensitivity, temporal resolution and simplicity. We demonstrate that OOTC can measure the dynamic mechanical modulus in the frequency range of 0.1-6,000 Hz at a rate as fast as 1 data point per second with submicron spatial resolution. More importantly, OOTC is capable of distinguishing the intrinsic non-random temporal variations from random fluctuations due to Brownian motion; this capability, not achievable by conventional approaches, is particular useful because living systems are highly dynamic and often exhibit non-thermal, rhythmic behavior in a broad time scale from a fraction of a second to hours or days. Although OOTC is effective in measuring the intracellular micromechanical properties, unless we can visualize the cytoskeleton in situ, the mechanical property data would only be as informative as that of "Blind men and the Elephant". To solve this problem, we take two steps, the first, to use of fluorescent imaging to identify the granular structures trapped by optical tweezers, and second, to integrate OOTC with 3-D confocal microscopy so we can take simultaneous, in situ measurements of the micromechanics and intracellular structure in living cells. In this paper, we discuss examples of applying the oscillating tweezer-based cytorheometer for investigating cultured bovine endothelial cells, the identification of caveolae as some of the granular structures in the cell as well as our approach to integrate optical tweezers with a spinning disk confocal microscope.

  13. On-machine measurement of the grinding wheels' 3D surface topography using a laser displacement sensor

    NASA Astrophysics Data System (ADS)

    Pan, Yongcheng; Zhao, Qingliang; Guo, Bing

    2014-08-01

    A method of non-contact, on-machine measurement of three dimensional surface topography of grinding wheels' whole surface was developed in this paper, focusing on an electroplated coarse-grained diamond grinding wheel. The measuring system consists of a Keyence laser displacement sensor, a Keyence controller and a NI PCI-6132 data acquisition card. A resolution of 0.1μm in vertical direction and 8μm in horizontal direction could be achieved. After processing the data by LabVIEW and MATLAB, the 3D topography of the grinding wheel's whole surface could be reconstructed. When comparing the reconstructed 3D topography of the grinding wheel's marked area to its real topography captured by a high-depth-field optical digital microscope (HDF-ODM) and scanning electron microscope (SEM), they were very similar to each other, proving that this method is accurate and effective. By a subsequent data processing, the topography of every grain could be extracted and then the active grain number, the active grain volume and the active grain's bearing ration could be calculated. These three parameters could serve as the criterion to evaluate the grinding performance of coarse-grained diamond grinding wheels. Then the performance of the grinding wheel could be evaluated on-machine accurately and quantitatively.

  14. Exploring the surface reactivity of 3d metal endofullerenes: a density-functional theory study.

    PubMed

    Estrada-Salas, Rubén E; Valladares, Ariel A

    2009-09-24

    Changes in the preferential sites of electrophilic, nucleophilic, and radical attacks on the pristine C60 surface with endohedral doping using 3d transition metal atoms were studied via two useful reactivity indices, namely the Fukui functions and the molecular electrostatic potential. Both of these were calculated at the density functional BPW91 level of theory with the DNP basis set. Our results clearly show changes in the preferential reactivity sites on the fullerene surface when it is doped with Mn, Fe, Co, or Ni atoms, whereas there are no significant changes in the preferential reactivity sites on the C60 surface upon endohedral doping with Cu and Zn atoms. Electron affinities (EA), ionization potentials (IP), and HOMO-LUMO gaps (Eg) were also calculated to complete the study of the endofullerene's surface reactivity. These findings provide insight into endofullerene functionalization, an important issue in their application.

  15. Assessment of engineered surfaces roughness by high-resolution 3D SEM photogrammetry.

    PubMed

    Gontard, L C; López-Castro, J D; González-Rovira, L; Vázquez-Martínez, J M; Varela-Feria, F M; Marcos, M; Calvino, J J

    2017-03-07

    We describe a methodology to obtain three-dimensional models of engineered surfaces using scanning electron microscopy and multi-view photogrammetry (3DSEM). For the reconstruction of the 3D models of the surfaces we used freeware available in the cloud. The method was applied to study the surface roughness of metallic samples patterned with parallel grooves by means of laser. The results are compared with measurements obtained using stylus profilometry (PR) and SEM stereo-photogrammetry (SP). The application of 3DSEM is more time demanding than PR or SP, but it provides a more accurate representation of the surfaces. The results obtained with the three techniques are compared by investigating the influence of sampling step on roughness parameters.

  16. Visualization of the 3-D topography of the optic nerve head through a passive stereo vision model

    NASA Astrophysics Data System (ADS)

    Ramirez, Juan M.; Mitra, Sunanda; Morales, Jose

    1999-01-01

    This paper describes a system for surface recovery and visualization of the 3D topography of the optic nerve head, as support of early diagnosis and follow up to glaucoma. In stereo vision, depth information is obtained from triangulation of corresponding points in a pair of stereo images. In this paper, the use of the cepstrum transformation as a disparity measurement technique between corresponding windows of different block sizes is described. This measurement process is embedded within a coarse-to-fine depth-from-stereo algorithm, providing an initial range map with the depth information encoded as gray levels. These sparse depth data are processed through a cubic B-spline interpolation technique in order to obtain a smoother representation. This methodology is being especially refined to be used with medical images for clinical evaluation of some eye diseases such as open angle glaucoma, and is currently under testing for clinical evaluation and analysis of reproducibility and accuracy.

  17. In-situ biofouling assessment in spacer filled channels using optical coherence tomography (OCT): 3D biofilm thickness mapping.

    PubMed

    Fortunato, Luca; Leiknes, TorOve

    2017-04-01

    Membrane systems for water purification can be seriously hampered by biofouling. The use of optical coherence tomography (OCT) to investigate biofilms in membrane systems has recently increased due to the ability to do the characterization in-situ and non-destructively. The OCT biofilm thickness map is presented for the first time as a tool to assess biofilm spatial distribution on a surface. The map allows the visualization and evaluation of the biofilm formation and growth in membrane filtration systems through the use of a false color scale. The biofilm development was monitored with OCT to evaluate the suitability of the proposed approach. A 3D time series analysis of biofilm development in a spacer filled channel representative of a spiral-wound membrane element was performed. The biofilm thickness map enables the time-resolved and spatial-resolved evaluation and visualization of the biofilm deposition pattern in-situ non-destructively.

  18. How to select the most relevant 3D roughness parameters of a surface.

    PubMed

    Deltombe, R; Kubiak, K J; Bigerelle, M

    2014-01-01

    In order to conduct a comprehensive roughness analysis, around sixty 3D roughness parameters are created to describe most of the surface morphology with regard to specific functions, properties or applications. In this paper, a multiscale surface topography decomposition method is proposed with application to stainless steel (AISI 304), which is processed by rolling at different fabrication stages and by electrical discharge tool machining. Fifty-six 3D-roughness parameters defined in ISO, EUR, and ASME standards are calculated for the measured surfaces. Then, expert software "MesRug" is employed to perform statistical analysis on acquired data in order to find the most relevant parameters characterizing the effect of both processes (rolling and machining), and to determine the most appropriate scale of analysis. For the rolling process: The parameter Vmc (the Core Material Volume--defined as volume of material comprising the texture between heights corresponding to the material ratio values of p = 10% and q = 80%) computed at the scale of 3 µm is the most relevant parameter to characterize the cold rolling process. For the EDM Process, the best roughness parameter is SPD that represents the number of peaks per unit area after segmentation of a surface into motifs computed at the scale of 8 µm.

  19. Beam position controlling method for 3D optical system and its application in non-planar ring resonators.

    PubMed

    Yuan, Jie; Chen, Meixiong; Long, Xingwu; Tan, Yanyang; Kang, Zhenglong; Li, Yingying

    2012-08-13

    A novel theoretical beam position controlling method for 3D optical system has been proposed in this paper. Non-planar ring resonator, which is a typical 3D optical system, has been chosen as an example to show its application. To the best of our knowledge, the generalized ray matrices, augmented 5 × 5 ray matrices for paraxial dielectric interface transmission and paraxial optical-wedge transmission, and their detailed deducing process have been proposed in this paper for the first time. By utilizing the novel coordinate system for Gaussian beam reflection and the generalized ray matrix of paraxial optical-wedge transmission, the rules and some novel results of the optical-axis perturbations of non-planar ring resonators have been obtained. Wedge angle-induced mismatching errors of non-planar ring resonators have been found out and two experimental beam position controlling methods to effectively eliminate the wedge angle-induced mismatching errors have been proposed. All those results have been confirmed by related alignment experiments and the experimental results have been described with diagrammatic representation. These findings are important to the beam control, cavity design, and cavity alignment of high precision non-planar ring laser gyroscopes. Those generalized ray matrices and their deducing methods are valuable for ray analysis of various kinds of paraxial optical-elements and resonators. This novel theoretical beam position controlling method for 3D optical system is valuable for the controlling of various kinds of 3D optical systems.

  20. 3-D imaging mass spectrometry of protein distributions in mouse Neurofibromatosis 1 (NF1)-associated optic glioma.

    PubMed

    Anderson, David M G; Van de Plas, Raf; Rose, Kristie L; Hill, Salisha; Schey, Kevin L; Solga, Anne C; Gutmann, David H; Caprioli, Richard M

    2016-10-21

    Neurofibromatosis type 1 (NF1) is a common neurogenetic disorder, in which affected individuals develop tumors of the nervous system. Children with NF1 are particularly prone to brain tumors (gliomas) involving the optic pathway that can result in impaired vision. Since tumor formation and expansion requires a cooperative tumor microenvironment, it is important to identify the cellular and acellular components associated with glioma development and growth. In this study, we used 3-D matrix assisted laser desorption ionization imaging mass spectrometry (MALDI IMS) to measure the distributions of multiple molecular species throughout optic nerve tissue in mice with and without glioma, and to explore their spatial relationships within the 3-D volume of the optic nerve and chiasm. 3-D IMS studies often involve extensive workflows due to the high volume of sections required to generate high quality 3-D images. Herein, we present a workflow for 3-D data acquisition and volume reconstruction using mouse optic nerve tissue. The resulting 3-D IMS data yield both molecular similarities and differences between glioma-bearing and wild-type (WT) tissues, including protein distributions localizing to different anatomical subregions.

  1. Beyond optical molasses: 3D raman sideband cooling of atomic cesium to high phase-space density

    PubMed

    Kerman; Vuletic; Chin; Chu

    2000-01-17

    We demonstrate a simple, general purpose method to cool neutral atoms. A sample containing 3x10(8) cesium atoms prepared in a magneto-optical trap is cooled and simultaneously spin polarized in 10 ms at a density of 1.1x10(11) cm (-3) to a phase space density nlambda(3)(dB) = 1/500, which is almost 3 orders of magnitude higher than attainable in free space with optical molasses. The technique is based on 3D degenerate Raman sideband cooling in optical lattices and remains efficient even at densities where the mean lattice site occupation is close to unity.

  2. Label-free optical detection of cells grown in 3D silicon microstructures.

    PubMed

    Merlo, Sabina; Carpignano, Francesca; Silva, Gloria; Aredia, Francesca; Scovassi, A Ivana; Mazzini, Giuliano; Surdo, Salvatore; Barillaro, Giuseppe

    2013-08-21

    We demonstrate high aspect-ratio photonic crystals that could serve as three-dimensional (3D) microincubators for cell culture and also provide label-free optical detection of the cells. The investigated microstructures, fabricated by electrochemical micromachining of standard silicon wafers, consist of periodic arrays of silicon walls separated by narrow deeply etched air-gaps (50 μm high and 5 μm wide) and feature the typical spectral properties of photonic crystals in the wavelength range 1.0-1.7 μm: their spectral reflectivity is characterized by wavelength regions where reflectivity is high (photonic bandgaps), separated by narrow wavelength regions where reflectivity is very low. In this work, we show that the presence of cells, grown inside the gaps, strongly affects light propagation across the photonic crystal and, therefore, its spectral reflectivity. Exploiting a label-free optical detection method, based on a fiberoptic setup, we are able to probe the extension of cells adherent to the vertical silicon walls with a non-invasive direct testing. In particular, the intensity ratio at two wavelengths is the experimental parameter that can be well correlated to the cell spreading on the silicon wall inside the gaps.

  3. 3D imaging of tomato seeds using frequency domain optical coherence tomography

    NASA Astrophysics Data System (ADS)

    Fan, Chuanmao; Yao, Gang

    2012-05-01

    A fast imaging system that can reveal internal sample structures is important for research and quality controls of seeds. Optical coherence tomography (OCT) is a non-invasive optical imaging technique that can acquire high speed, high resolution depth-resolved images in scattering samples. It has found numerous applications in studying various biological tissues and other materials in vivo. A few studies have reported the use of OCT in studying seed morphology. However, 3D imaging of internal seed structure has not been reported before. In this study, we used a frequency domain OCT system to image tomato seeds. The system has a central wavelength of 844nm with a 46.8 nm FWHM bandwidth. The requirement for depth scan was eliminated by using a Fourier domain implementation. The B-scan imaging speed was limited by the spectroscopic imaging CCD at 52 kHz. The calibrated system has a 6.7μm depth resolution and a 15.4μm lateral resolution. Our results show that major seed structures can be clearly visualized in OCT images.

  4. Anomalous surface segregation behaviour of some 3d elements in ferromagnetic iron.

    PubMed

    Gupta, Michèle; Gupta, Raju P

    2013-10-16

    The segregation of Cr in Fe is known to be anomalous since the barrier for surface segregation of Cr is not determined by the topmost surface layer, as one would expect, but rather by the subsurface layer where the energy of segregation is much larger and endothermic. This has been attributed to a complex interaction involving the antiferromagnetism of Cr and the ferromagnetism of Fe. We report in this paper the results of our ab initio electronic structure calculations on the segregation behaviour of all the 3d elements on the (1 0 0) surface of ferromagnetic iron in the hope of better understanding this phenomenon. We find a similar behaviour for the segregation of the next antiferromagnetic 3d element Mn in Fe, where the subsurface layer is also found to block the segregation of Mn to the surface. On the other hand, ferromagnetic Co exhibits a normal segregation behaviour. The elements Sc, Cu and Ni do not form solid solutions with ferromagnetic iron. The early elements Ti and V are non-magnetic in their metallic states, but are strongly polarized by Fe, and develop magnetic moments which are aligned antiferromagnetically to those of Fe atoms. While the subsurface layer blocks the segregation of Ti to the surface, no blocking behaviour is found for the segregation of V. The segregation behaviour of all these elements is strongly correlated with the displacement of the solute atoms on the surface of Fe. The elements showing anomalous segregation behaviour are all displaced upwards on the surface, while those showing normal segregation are pulled inwards. These results indicate that the antiferromagnetism of the segregating element plays the key role in the anomalous segregation behaviour in Fe.

  5. Additive manufactured polymeric 3D scaffolds with tailored surface topography influence mesenchymal stromal cells activity.

    PubMed

    Neves, Sara C; Mota, Carlos; Longoni, Alessia; Barrias, Cristina C; Granja, Pedro L; Moroni, Lorenzo

    2016-05-24

    Additive manufactured three-dimensional (3D) scaffolds with tailored surface topography constitute a clear advantage in tissue regeneration strategies to steer cell behavior. 3D fibrous scaffolds of poly(ethylene oxide terephthalate)/poly(butylene terephthalate) block copolymer presenting different fiber surface features were successfully fabricated by additive manufacturing combined with wet-spinning, in a single step, without any post-processing. The optimization of the processing parameters, mainly driven by different solvent/non-solvent combinations, led to four distinct scaffold types, with average surface roughness values ranging from 0.071 ± 0.012 μm to 1.950 ± 0.553 μm, average pore sizes in the x- and y-axis between 351.1 ± 33.6 μm and 396.1 ± 32.3 μm, in the z-axis between 36.5 ± 5.3 μm and 70.7 ± 8.8 μm, average fiber diameters between 69.4 ± 6.1 μm and 99.0 ± 9.4 μm, and porosity values ranging from 60.2 ± 0.8% to 71.7 ± 2.6%. Human mesenchymal stromal cells (hMSCs) cultured on these scaffolds adhered, proliferated, and produced endogenous extracellular matrix. The effect of surface roughness and topography on hMSCs differentiation was more evident for cells seeded at lower density, where the percentage of cells in direct contact with the surface was higher compared to more densely seeded scaffolds. Under osteogenic conditions, lower surface roughness values (0.227 ± 0.035 μm) had a synergistic effect on hMSCs behavior, while chondrogenesis was favored on rougher surfaces (1.950 ± 0.553 μm).

  6. Real-time 3D Fourier-domain optical coherence tomography guided microvascular anastomosis

    NASA Astrophysics Data System (ADS)

    Huang, Yong; Ibrahim, Zuhaib; Lee, W. P. A.; Brandacher, Gerald; Kang, Jin U.

    2013-03-01

    Vascular and microvascular anastomosis is considered to be the foundation of plastic and reconstructive surgery, hand surgery, transplant surgery, vascular surgery and cardiac surgery. In the last two decades innovative techniques, such as vascular coupling devices, thermo-reversible poloxamers and suture-less cuff have been introduced. Intra-operative surgical guidance using a surgical imaging modality that provides in-depth view and 3D imaging can improve outcome following both conventional and innovative anastomosis techniques. Optical coherence tomography (OCT) is a noninvasive high-resolution (micron level), high-speed, 3D imaging modality that has been adopted widely in biomedical and clinical applications. In this work we performed a proof-of-concept evaluation study of OCT as an assisted intraoperative and post-operative imaging modality for microvascular anastomosis of rodent femoral vessels. The OCT imaging modality provided lateral resolution of 12 μm and 3.0 μm axial resolution in air and 0.27 volume/s imaging speed, which could provide the surgeon with clearly visualized vessel lumen wall and suture needle position relative to the vessel during intraoperative imaging. Graphics processing unit (GPU) accelerated phase-resolved Doppler OCT (PRDOCT) imaging of the surgical site was performed as a post-operative evaluation of the anastomosed vessels and to visualize the blood flow and thrombus formation. This information could help surgeons improve surgical precision in this highly challenging anastomosis of rodent vessels with diameter less than 0.5 mm. Our imaging modality could not only detect accidental suture through the back wall of lumen but also promptly diagnose and predict thrombosis immediately after reperfusion. Hence, real-time OCT can assist in decision-making process intra-operatively and avoid post-operative complications.

  7. Nonlinear Optical Macroscopic Assessment of 3-D Corneal Collagen Organization and Axial Biomechanics

    PubMed Central

    Winkler, Moritz; Chai, Dongyul; Kriling, Shelsea; Nien, Chyong Jy; Brown, Donald J.; Jester, Bryan; Juhasz, Tibor

    2011-01-01

    Purpose. To characterize and quantify the collagen fiber (lamellar) organization of human corneas in three dimensions by using nonlinear optical high-resolution macroscopy (NLO-HRMac) and to correlate these findings with mechanical data obtained by indentation testing of corneal flaps. Methods. Twelve corneas from 10 donors were studied. Vibratome sections, 200 μm thick, from five donor eyes were cut along the vertical meridian from limbus to limbus (arc length, 12 mm). Backscattered second harmonic–generated (SHG) NLO signals from these sections were collected as a series of overlapping 3-D images, which were concatenated to form a single 3-D mosaic (pixel resolution: 0.44 μm lateral, 2 μm axial). Collagen fiber intertwining was quantified by determining branching point density as a function of stromal depth. Mechanical testing was performed on corneal flaps from seven additional eyes. Corneas were cut into three layers (anterior, middle, and posterior) using a femtosecond surgical laser system and underwent indentation testing to determine the elastic modulus for each layer. Results. The 3-D reconstructions revealed complex collagen fiber branching patterns in the anterior cornea, with fibers extending from the anterior limiting lamina (ALL, Bowman's layer), intertwining with deeper fibers and reinserting back to the ALL, forming bow spring–like structures. Measured branching-point density was four times higher in the anterior third of the cornea than in the posterior third and decreased logarithmically with increasing distance from the ALL. Indentation testing showed an eightfold increase in elastic modulus in the anterior stroma. Conclusions. The axial gradient in lamellar intertwining appears to be associated with an axial gradient in the effective elastic modulus of the cornea, suggesting that collagen fiber intertwining and formation of bow spring–like structures provide structural support similar to cross-beams in bridges and large-scale structures

  8. Optical Measurement of Micromechanics and Structure in a 3D Fibrin Extracellular Matrix

    NASA Astrophysics Data System (ADS)

    Kotlarchyk, Maxwell Aaron

    2011-07-01

    In recent years, a significant number of studies have focused on linking substrate mechanics to cell function using standard methodologies to characterize the bulk properties of the hydrogel substrates. However, current understanding of the correlations between the microstructural mechanical properties of hydrogels and cell function in 3D is poor, in part because of a lack of appropriate techniques. Methods for tuning extracellular matrix (ECM) mechanics in 3D cell culture that rely on increasing the concentration of either protein or cross-linking molecules fail to control important parameters such as pore size, ligand density, and molecular diffusivity. Alternatively, ECM stiffness can be modulated independently from protein concentration by mechanically loading the ECM. We have developed an optical tweezers-based microrheology system to investigate the fundamental role of ECM mechanical properties in determining cellular behavior. Further, this thesis outlines the development of a novel device for generating stiffness gradients in naturally derived ECMs, where stiffness is tuned by inducing strain, while local structure and mechanical properties are directly determined by laser tweezers-based passive and active microrheology respectively. Hydrogel substrates polymerized within 35 mm diameter Petri dishes are strained non-uniformly by the precise rotation of an embedded cylindrical post, and exhibit a position-dependent stiffness with little to no modulation of local mesh geometry. Here we present microrheological studies in the context of fibrin hydrogels. Microrheology and confocal imaging were used to directly measure local changes in micromechanics and structure respectively in unstrained hydrogels of increasing fibrinogen concentration, as well as in our strain gradient device, in which the concentration of fibrinogen is held constant. Orbital particle tracking, and raster image correlation analysis are used to quantify changes in fibrin mechanics on the

  9. 3D modelling of near-surface, environmental effects on AEM data

    NASA Astrophysics Data System (ADS)

    Beamish, David

    2004-11-01

    This study considers the three-dimensional (3D) modelling of compact, at-surface conductive bodies on frequency domain airborne electromagnetic (AEM) survey data. The context is the use of AEM data for environmental and land quality applications. The 3D structures encountered are typically conductive, of limited thickness (<20 m) and form 'point' source locations carrying potential environmental risk. The scale of such bodies may generate single-profile, 'bulls-eye' anomalies. In attempts to recover geological information, such anomalies may be considered to represent noise. In environmental AEM, the correct interpretation of such features is important. The study uses a combination of theoretical models and trial-fixed-wing survey data obtained in populated areas of the UK. Scale issues are discussed in terms of the volumetric footprints of the induced electric field generated by systems flown at both low and high elevation. One of the primary uses of AEM survey data lies in the assessment of conductivity maps. These are typically obtained using one-dimensional (1D) conductivity models at individual measurement points. In order to investigate the limitations of this approach, 3D modelling of conductive structures with dimensions less than 350×350 m and thicknesses extending to 20 m has been carried out. A 1D half space inversion of the data obtained at each frequency is then used to assess the behaviour of the spatial information. The results demonstrate that half space conductivity values obtained over compact 3D targets generally provide only apparent conductivity results. For thin, at-surface bodies, conductivity values are biased to lower values than the true conductivity except at high frequency. The spatial perturbation to both coupling ratios and 1D conductivity models can be laterally extensive. The results from 3D modelling indicate that the use of horizontal derivatives applied to the conductivity models offers enhanced edge detection. The practical

  10. Robust statistical approaches for local planar surface fitting in 3D laser scanning data

    NASA Astrophysics Data System (ADS)

    Nurunnabi, Abdul; Belton, David; West, Geoff

    2014-10-01

    This paper proposes robust methods for local planar surface fitting in 3D laser scanning data. Searching through the literature revealed that many authors frequently used Least Squares (LS) and Principal Component Analysis (PCA) for point cloud processing without any treatment of outliers. It is known that LS and PCA are sensitive to outliers and can give inconsistent and misleading estimates. RANdom SAmple Consensus (RANSAC) is one of the most well-known robust methods used for model fitting when noise and/or outliers are present. We concentrate on the recently introduced Deterministic Minimum Covariance Determinant estimator and robust PCA, and propose two variants of statistically robust algorithms for fitting planar surfaces to 3D laser scanning point cloud data. The performance of the proposed robust methods is demonstrated by qualitative and quantitative analysis through several synthetic and mobile laser scanning 3D data sets for different applications. Using simulated data, and comparisons with LS, PCA, RANSAC, variants of RANSAC and other robust statistical methods, we demonstrate that the new algorithms are significantly more efficient, faster, and produce more accurate fits and robust local statistics (e.g. surface normals), necessary for many point cloud processing tasks. Consider one example data set used consisting of 100 points with 20% outliers representing a plane. The proposed methods called DetRD-PCA and DetRPCA, produce bias angles (angle between the fitted planes with and without outliers) of 0.20° and 0.24° respectively, whereas LS, PCA and RANSAC produce worse bias angles of 52.49°, 39.55° and 0.79° respectively. In terms of speed, DetRD-PCA takes 0.033 s on average for fitting a plane, which is approximately 6.5, 25.4 and 25.8 times faster than RANSAC, and two other robust statistical methods, respectively. The estimated robust surface normals and curvatures from the new methods have been used for plane fitting, sharp feature

  11. Mapping gray-scale image to 3D surface scanning data by ray tracing

    NASA Astrophysics Data System (ADS)

    Li, Peng; Jones, Peter R. M.

    1997-03-01

    The extraction and location of feature points from range imaging is an important but difficult task in machine vision based measurement systems. There exist some feature points which are not able to be detected from pure geometric characteristics, particularly in those measurement tasks related to the human body. The Loughborough Anthropometric Shadow Scanner (LASS) is a whole body surface scanner based on structured light technique. Certain applications of LASS require accurate location of anthropometric landmarks from the scanned data. This is sometimes impossible from existing raw data because some landmarks do not appear in the scanned data. Identification of these landmarks has to resort to surface texture of the scanned object. Modifications to LASS were made to allow gray-scale images to be captured before or after the object was scanned. Two-dimensional gray-scale image must be mapped to the scanned data to acquire the 3D coordinates of a landmark. The method to map 2D images to the scanned data is based on the colinearity conditions and ray-tracing method. If the camera center and image coordinates are known, the corresponding object point must lie on a ray starting from the camera center and connecting to the image coordinate. By intersecting the ray with the scanned surface of the object, the 3D coordinates of a point can be solved. Experimentation has demonstrated the feasibility of the method.

  12. Performance analysis of different surface reconstruction algorithms for 3D reconstruction of outdoor objects from their digital images.

    PubMed

    Maiti, Abhik; Chakravarty, Debashish

    2016-01-01

    3D reconstruction of geo-objects from their digital images is a time-efficient and convenient way of studying the structural features of the object being modelled. This paper presents a 3D reconstruction methodology which can be used to generate photo-realistic 3D watertight surface of different irregular shaped objects, from digital image sequences of the objects. The 3D reconstruction approach described here is robust, simplistic and can be readily used in reconstructing watertight 3D surface of any object from its digital image sequence. Here, digital images of different objects are used to build sparse, followed by dense 3D point clouds of the objects. These image-obtained point clouds are then used for generation of photo-realistic 3D surfaces, using different surface reconstruction algorithms such as Poisson reconstruction and Ball-pivoting algorithm. Different control parameters of these algorithms are identified, which affect the quality and computation time of the reconstructed 3D surface. The effects of these control parameters in generation of 3D surface from point clouds of different density are studied. It is shown that the reconstructed surface quality of Poisson reconstruction depends on Samples per node (SN) significantly, greater SN values resulting in better quality surfaces. Also, the quality of the 3D surface generated using Ball-Pivoting algorithm is found to be highly depend upon Clustering radius and Angle threshold values. The results obtained from this study give the readers of the article a valuable insight into the effects of different control parameters on determining the reconstructed surface quality.

  13. Measuring surface topography with scanning electron microscopy. I. EZEImage: a program to obtain 3D surface data.

    PubMed

    Ponz, Ezequiel; Ladaga, Juan Luis; Bonetto, Rita Dominga

    2006-04-01

    Scanning electron microscopy (SEM) is widely used in the science of materials and different parameters were developed to characterize the surface roughness. In a previous work, we studied the surface topography with fractal dimension at low scale and two parameters at high scale by using the variogram, that is, variance vs. step log-log graph, of a SEM image. Those studies were carried out with the FERImage program, previously developed by us. To verify the previously accepted hypothesis by working with only an image, it is indispensable to have reliable three-dimensional (3D) surface data. In this work, a new program (EZEImage) to characterize 3D surface topography in SEM has been developed. It uses fast cross correlation and dynamic programming to obtain reliable dense height maps in a few seconds which can be displayed as an image where each gray level represents a height value. This image can be used for the FERImage program or any other software to obtain surface topography characteristics. EZEImage also generates anaglyph images as well as characterizes 3D surface topography by means of a parameter set to describe amplitude properties and three functional indices for characterizing bearing and fluid properties.

  14. An adaptive learning approach for 3-D surface reconstruction from point clouds.

    PubMed

    Junior, Agostinho de Medeiros Brito; Neto, Adrião Duarte Dória; de Melo, Jorge Dantas; Goncalves, Luiz Marcos Garcia

    2008-06-01

    In this paper, we propose a multiresolution approach for surface reconstruction from clouds of unorganized points representing an object surface in 3-D space. The proposed method uses a set of mesh operators and simple rules for selective mesh refinement, with a strategy based on Kohonen's self-organizing map (SOM). Basically, a self-adaptive scheme is used for iteratively moving vertices of an initial simple mesh in the direction of the set of points, ideally the object boundary. Successive refinement and motion of vertices are applied leading to a more detailed surface, in a multiresolution, iterative scheme. Reconstruction was experimented on with several point sets, including different shapes and sizes. Results show generated meshes very close to object final shapes. We include measures of performance and discuss robustness.

  15. Highly effective surface-enhanced fluorescence substrates with roughened 3D flowerlike silver nanostructures fabricated in liquid crystalline phase

    NASA Astrophysics Data System (ADS)

    Zhang, Ying; Yang, Chengliang; Xiang, Xiangjun; Zhang, Peiguang; Peng, Zenghui; Cao, Zhaoliang; Mu, Quanquan; Xuan, Li

    2017-04-01

    Highly effective surface-enhanced fluorescence substrates with roughened 3D flowerlike silver nanostructures were fabricated by electrodeposition in liquid crystalline template which is simple and controllable. Due to the localized surface plasmon resonance of silver nanostructures, the substrates were used as surface enhanced fluorescence substrates. The morphology and optical properties of the substrates were studied. The fluorescence experiments of the Rhodamine 6G on the substrates for different growth times were carried out and the best enhancement factor of 181 was achieved. Eight substrates with the same growth conditions were used to study the reproducibility of the substrate which shows that the fluctuations are within 9%. This substrate was used in organic distributed feedback lasers and the amplified spontaneous emission of poly(2-methoxy-5-(2‧-ethyl-hexyloxy)-p-phenylenevinylene) was enhanced dramatically which means the reduced threshold and improved slope efficiency. Such easily fabricated flower-like silver nanostructure substrates with strong surface enhanced fluorescence effect and good reproducibility are good candidate for potential applications in optical imaging, biotechnology and material detections.

  16. Active surface model improvement by energy function optimization for 3D segmentation.

    PubMed

    Azimifar, Zohreh; Mohaddesi, Mahsa

    2015-04-01

    This paper proposes an optimized and efficient active surface model by improving the energy functions, searching method, neighborhood definition and resampling criterion. Extracting an accurate surface of the desired object from a number of 3D images using active surface and deformable models plays an important role in computer vision especially medical image processing. Different powerful segmentation algorithms have been suggested to address the limitations associated with the model initialization, poor convergence to surface concavities and slow convergence rate. This paper proposes a method to improve one of the strongest and recent segmentation algorithms, namely the Decoupled Active Surface (DAS) method. We consider a gradient of wavelet edge extracted image and local phase coherence as external energy to extract more information from images and we use curvature integral as internal energy to focus on high curvature region extraction. Similarly, we use resampling of points and a line search for point selection to improve the accuracy of the algorithm. We further employ an estimation of the desired object as an initialization for the active surface model. A number of tests and experiments have been done and the results show the improvements with regards to the extracted surface accuracy and computational time of the presented algorithm compared with the best and recent active surface models.

  17. Feature-constrained surface reconstruction approach for point cloud data acquired with 3D laser scanner

    NASA Astrophysics Data System (ADS)

    Wang, Yongbo; Sheng, Yehua; Lu, Guonian; Tian, Peng; Zhang, Kai

    2008-04-01

    Surface reconstruction is an important task in the field of 3d-GIS, computer aided design and computer graphics (CAD & CG), virtual simulation and so on. Based on available incremental surface reconstruction methods, a feature-constrained surface reconstruction approach for point cloud is presented. Firstly features are extracted from point cloud under the rules of curvature extremes and minimum spanning tree. By projecting local sample points to the fitted tangent planes and using extracted features to guide and constrain the process of local triangulation and surface propagation, topological relationship among sample points can be achieved. For the constructed models, a process named consistent normal adjustment and regularization is adopted to adjust normal of each face so that the correct surface model is achieved. Experiments show that the presented approach inherits the convenient implementation and high efficiency of traditional incremental surface reconstruction method, meanwhile, it avoids improper propagation of normal across sharp edges, which means the applicability of incremental surface reconstruction is greatly improved. Above all, appropriate k-neighborhood can help to recognize un-sufficient sampled areas and boundary parts, the presented approach can be used to reconstruct both open and close surfaces without additional interference.

  18. 3D Cloud Radiative Effects on Aerosol Optical Thickness Retrievals in Cumulus Cloud Fields in the Biomass Burning Region in Brazil

    NASA Technical Reports Server (NTRS)

    Wen, Guo-Yong; Marshak, Alexander; Cahalan, Robert F.

    2004-01-01

    Aerosol amount in clear regions of a cloudy atmosphere is a critical parameter in studying the interaction between aerosols and clouds. Since the global cloud cover is about 50%, cloudy scenes are often encountered in any satellite images. Aerosols are more or less transparent, while clouds are extremely reflective in the visible spectrum of solar radiation. The radiative transfer in clear-cloudy condition is highly three- dimensional (3D). This paper focuses on estimating the 3D effects on aerosol optical thickness retrievals using Monte Carlo simulations. An ASTER image of cumulus cloud fields in the biomass burning region in Brazil is simulated in this study. The MODIS products (i-e., cloud optical thickness, particle effective radius, cloud top pressure, surface reflectance, etc.) are used to construct the cloud property and surface reflectance fields. To estimate the cloud 3-D effects, we assume a plane-parallel stratification of aerosol properties in the 60 km x 60 km ASTER image. The simulated solar radiation at the top of the atmosphere is compared with plane-parallel calculations. Furthermore, the 3D cloud radiative effects on aerosol optical thickness retrieval are estimated.

  19. A prototype fan-beam optical CT scanner for 3D dosimetry

    SciTech Connect

    Campbell, Warren G.; Rudko, D. A.; Braam, Nicolas A.; Jirasek, Andrew; Wells, Derek M.

    2013-06-15

    Purpose: The objective of this work is to introduce a prototype fan-beam optical computed tomography scanner for three-dimensional (3D) radiation dosimetry. Methods: Two techniques of fan-beam creation were evaluated: a helium-neon laser (HeNe, {lambda} = 543 nm) with line-generating lens, and a laser diode module (LDM, {lambda} = 635 nm) with line-creating head module. Two physical collimator designs were assessed: a single-slot collimator and a multihole collimator. Optimal collimator depth was determined by observing the signal of a single photodiode with varying collimator depths. A method of extending the dynamic range of the system is presented. Two sample types were used for evaluations: nondosimetric absorbent solutions and irradiated polymer gel dosimeters, each housed in 1 liter cylindrical plastic flasks. Imaging protocol investigations were performed to address ring artefacts and image noise. Two image artefact removal techniques were performed in sinogram space. Collimator efficacy was evaluated by imaging highly opaque samples of scatter-based and absorption-based solutions. A noise-based flask registration technique was developed. Two protocols for gel manufacture were examined. Results: The LDM proved advantageous over the HeNe laser due to its reduced noise. Also, the LDM uses a wavelength more suitable for the PRESAGE{sup TM} dosimeter. Collimator depth of 1.5 cm was found to be an optimal balance between scatter rejection, signal strength, and manufacture ease. The multihole collimator is capable of maintaining accurate scatter-rejection to high levels of opacity with scatter-based solutions (T < 0.015%). Imaging protocol investigations support the need for preirradiation and postirradiation scanning to reduce reflection-based ring artefacts and to accommodate flask imperfections and gel inhomogeneities. Artefact removal techniques in sinogram space eliminate streaking artefacts and reduce ring artefacts of up to {approx}40% in magnitude. The

  20. Robust affine-invariant feature points matching for 3D surface reconstruction of complex landslide scenes

    NASA Astrophysics Data System (ADS)

    Stumpf, André; Malet, Jean-Philippe; Allemand, Pascal; Skupinski, Grzegorz; Deseilligny, Marc-Pierrot

    2013-04-01

    Multi-view stereo surface reconstruction from dense terrestrial photographs is being increasingly applied for geoscience applications such as quantitative geomorphology, and a number of different software solution and processing streamlines have been suggested. For image matching, camera self-calibration and bundle block adjustment, most approaches make use of scale-invariant feature transform (SIFT) to identify homologous points in multiple images. SIFT-like point matching is robust to apparent translation, rotation, and scaling of objects in multiple viewing geometries but the number of correctly identified matching points typically declines drastically with increasing angles between the viewpoints. For the application of multi-view stereo of complex landslide scenes, the viewing geometry is often constrained by the local topography and barriers such as rocks and vegetation occluding the target. Under such conditions it is not uncommon to encounter view angle differences of > 30% that hinder the image matching and eventually prohibit the joint estimation of the camera parameters from all views. Recently an affine invariant extension of the SIFT detector (ASIFT) has been demonstrated to provide more robust matches when large view-angle differences become an issue. In this study the ASIFT detector was adopted to detect homologous points in terrestrial photographs preceding 3D reconstruction of different parts (main scarp, toe) of the Super-Sauze landslide (Southern French Alps). 3D surface models for different time periods and different parts of the landslide were derived using the multi-view stereo framework implemented in MicMac (©IGN). The obtained 3D models were compared with reconstructions using the traditional SIFT detectors as well as alternative structure-from-motion implementations. An estimate of the absolute accuracy of the photogrammetric models was obtained through co-registration and comparison with high-resolution terrestrial LiDAR scans.

  1. Persistent and automatic intraoperative 3D digitization of surfaces under dynamic magnifications of an operating microscope.

    PubMed

    Kumar, Ankur N; Miga, Michael I; Pheiffer, Thomas S; Chambless, Lola B; Thompson, Reid C; Dawant, Benoit M

    2015-01-01

    One of the major challenges impeding advancement in image-guided surgical (IGS) systems is the soft-tissue deformation during surgical procedures. These deformations reduce the utility of the patient's preoperative images and may produce inaccuracies in the application of preoperative surgical plans. Solutions to compensate for the tissue deformations include the acquisition of intraoperative tomographic images of the whole organ for direct displacement measurement and techniques that combines intraoperative organ surface measurements with computational biomechanical models to predict subsurface displacements. The later solution has the advantage of being less expensive and amenable to surgical workflow. Several modalities such as textured laser scanners, conoscopic holography, and stereo-pair cameras have been proposed for the intraoperative 3D estimation of organ surfaces to drive patient-specific biomechanical models for the intraoperative update of preoperative images. Though each modality has its respective advantages and disadvantages, stereo-pair camera approaches used within a standard operating microscope is the focus of this article. A new method that permits the automatic and near real-time estimation of 3D surfaces (at 1 Hz) under varying magnifications of the operating microscope is proposed. This method has been evaluated on a CAD phantom object and on full-length neurosurgery video sequences (∼1 h) acquired intraoperatively by the proposed stereovision system. To the best of our knowledge, this type of validation study on full-length brain tumor surgery videos has not been done before. The method for estimating the unknown magnification factor of the operating microscope achieves accuracy within 0.02 of the theoretical value on a CAD phantom and within 0.06 on 4 clinical videos of the entire brain tumor surgery. When compared to a laser range scanner, the proposed method for reconstructing 3D surfaces intraoperatively achieves root mean square

  2. Construction of 3D micropatterned surfaces with wormlike and superhydrophilic PEG brushes to detect dysfunctional cells.

    PubMed

    Hou, Jianwen; Shi, Qiang; Ye, Wei; Fan, Qunfu; Shi, Hengchong; Wong, Shing-Chung; Xu, Xiaodong; Yin, Jinghua

    2014-12-10

    Detection of dysfunctional and apoptotic cells plays an important role in clinical diagnosis and therapy. To develop a portable and user-friendly platform for dysfunctional and aging cell detection, we present a facile method to construct 3D patterns on the surface of styrene-b-(ethylene-co-butylene)-b-styrene elastomer (SEBS) with poly(ethylene glycol) brushes. Normal red blood cells (RBCs) and lysed RBCs (dysfunctional cells) are used as model cells. The strategy is based on the fact that poly(ethylene glycol) brushes tend to interact with phosphatidylserine, which is in the inner leaflet of normal cell membranes but becomes exposed in abnormal or apoptotic cell membranes. We demonstrate that varied patterned surfaces can be obtained by selectively patterning atom transfer radical polymerization (ATRP) initiators on the SEBS surface via an aqueous-based method and growing PEG brushes through surface-initiated atom transfer radical polymerization. The relatively high initiator density and polymerization temperature facilitate formation of PEG brushes in high density, which gives brushes worm-like morphology and superhydrophilic property; the tendency of dysfunctional cells adhered on the patterned surfaces is completely different from well-defined arrays of normal cells on the patterned surfaces, providing a facile method to detect dysfunctional cells effectively. The PEG-patterned surfaces are also applicable to detect apoptotic HeLa cells. The simplicity and easy handling of the described technique shows the potential application in microdiagnostic devices.

  3. Reliability of trunk shape measurements based on 3-D surface reconstructions

    PubMed Central

    Cheriet, Farida; Danserau, Jean; Ronsky, Janet; Zernicke, Ronald F.; Labelle, Hubert

    2007-01-01

    This study aimed to estimate the reliability of 3-D trunk surface measurements for the characterization of external asymmetry associated with scoliosis. Repeated trunk surface acquisitions using the Inspeck system (Inspeck Inc., Montreal, Canada), with two different postures A (anatomical position) and B (‘‘clavicle’’ position), were obtained from patients attending a scoliosis clinic. For each acquisition, a 3-D model of the patient’s trunk was built and a series of measurements was computed. For each measure and posture, intraclass correlation coefficients (ICC) were obtained using a bivariate analysis of variance, and the smallest detectable difference was calculated. For posture A, reliability was fair to excellent with ICC from 0.91 to 0.99 (0.85 to 0.99 for the lower bound of the 95% confidence interval). For posture B, the ICC was 0.85 to 0.98 (0.74 to 0.99 for the lower bound of the 95% confidence interval). The smallest statistically significant differences for the maximal back surface rotation was 2.5 and 1.5° for the maximal trunk rotation. Apparent global asymmetry and axial trunk rotation indices were relatively robust to changes in arm posture, both in terms of mean values and within-subject variations, and also showed a good reliability. Computing measurements from cross-sectional analysis enabled a reduction in errors compared to the measurements based on markers’ position. Although not yet sensitive enough to detect small changes for monitoring of curve natural progression, trunk surface analysis can help to document the external asymmetry associated with different types of spinal curves as well as the cosmetic improvement obtained after surgical interventions. The anatomical posture is slightly more reliable as it allows a better coverage of the trunk surface by the digitizing system. PMID:17701228

  4. Effects of scanning orientation on outlier formation in 3D laser scanning of reflective surfaces

    NASA Astrophysics Data System (ADS)

    Wang, Yutao; Feng, Hsi-Yung

    2016-06-01

    Inspecting objects with reflective surfaces using 3D laser scanning is a demanded but challenging part inspection task due to undesirable specular reflections, which produce extensive outliers in the scanned point cloud. These outliers need to be removed in order to alleviate subsequent data processing issues. Many existing automatic outlier removal methods do not detect outliers according to the outlier formation properties. As a result, these methods only offer limited capabilities in removing extensive and complex outliers from scanning objects with reflective surfaces. This paper reports an empirical study which experimentally investigates the outlier formation characteristics in relation to the scanning orientation of the laser probe. The objective is to characterize the scanning orientation effects on outlier formation in order to facilitate the development of an effective outlier detection and removal method. Such an experimental investigation was hardly done before. It has been found in this work that scanning orientation can directly affect outlier extensity and occurrence in 3D laser scanning. A general guidance on proper scan path planning can then be provided with an aim to reduce the occurrence of outliers. Further, the observed dependency of outlier formation on scanning orientation can be exploited to facilitate effective and automatic outlier detection and removal.

  5. Numerical and experimental investigation of the 3D free surface flow in a model Pelton turbine

    NASA Astrophysics Data System (ADS)

    Fiereder, R.; Riemann, S.; Schilling, R.

    2010-08-01

    This investigation focuses on the numerical and experimental analysis of the 3D free surface flow in a Pelton turbine. In particular, two typical flow conditions occurring in a full scale Pelton turbine - a configuration with a straight inlet as well as a configuration with a 90 degree elbow upstream of the nozzle - are considered. Thereby, the effect of secondary flow due to the 90 degree bending of the upstream pipe on the characteristics of the jet is explored. The hybrid flow field consists of pure liquid flow within the conduit and free surface two component flow of the liquid jet emerging out of the nozzle into air. The numerical results are validated against experimental investigations performed in the laboratory of the Institute of Fluid Mechanics (FLM). For the numerical simulation of the flow the in-house unstructured fully parallelized finite volume solver solver3D is utilized. An advanced interface capturing model based on the classic Volume of Fluid method is applied. In order to ensure sharp interface resolution an additional convection term is added to the transport equation of the volume fraction. A collocated variable arrangement is used and the set of non-linear equations, containing fluid conservation equations and model equations for turbulence and volume fraction, are solved in a segregated manner. For pressure-velocity coupling the SIMPLE and PISO algorithms are implemented. Detailed analysis of the observed flow patterns in the jet and of the jet geometry are presented.

  6. Surface rippling during solidification of binary polycrystalline alloy: Insights from 3-D phase-field simulations

    NASA Astrophysics Data System (ADS)

    Ankit, Kumar; Xing, Hui; Selzer, Michael; Nestler, Britta; Glicksman, Martin E.

    2017-01-01

    The mechanisms by which crystalline imperfections initiate breakdown of a planar front during directional solidification remain a topic of longstanding interest. Previous experimental findings show that the solid-liquid interface adjacent to a grain boundary provides a potential site where morphological instabilities initiate. However, interpretation of experimental data is difficult for complex 3-D diffusion fields that develop around grain multi-junctions and boundary ridges. We apply a phase-field approach to investigate factors that induce interfacial instabilities during directional solidification of a binary polycrystalline alloy. Using 2-D simulations, we establish the influence of solid-liquid interfacial energies on the spatial localization of initial interfacial perturbations. Based on parametric studies, we predict that grain misorientation and supersaturation in the melt provide major crystal growth factors determining solute segregation responsible for surface rippling. Subsequent breakdown of boundary ridges into periodic rows of hills, as simulated in 3-D, conform well with experiments. Finally, the significance of crystal misorientation relationships is elucidated in inducing spatial alignment of surface ripples.

  7. Spatially monitoring oxygen level in 3D microfabricated cell culture systems using optical oxygen sensing beads.

    PubMed

    Wang, Lin; Acosta, Miguel A; Leach, Jennie B; Carrier, Rebecca L

    2013-04-21

    Capability of measuring and monitoring local oxygen concentration at the single cell level (tens of microns scale) is often desirable but difficult to achieve in cell culture. In this study, biocompatible oxygen sensing beads were prepared and tested for their potential for real-time monitoring and mapping of local oxygen concentration in 3D micro-patterned cell culture systems. Each oxygen sensing bead is composed of a silica core loaded with both an oxygen sensitive Ru(Ph2phen3)Cl2 dye and oxygen insensitive Nile blue reference dye, and a poly-dimethylsiloxane (PDMS) shell rendering biocompatibility. Human intestinal epithelial Caco-2 cells were cultivated on a series of PDMS and type I collagen based substrates patterned with micro-well arrays for 3 or 7 days, and then brought into contact with oxygen sensing beads. Using an image analysis algorithm to convert florescence intensity of beads to partial oxygen pressure in the culture system, tens of microns-size oxygen sensing beads enabled the spatial measurement of local oxygen concentration in the microfabricated system. Results generally indicated lower oxygen level inside wells than on top of wells, and local oxygen level dependence on structural features of cell culture surfaces. Interestingly, chemical composition of cell culture substrates also appeared to affect oxygen level, with type-I collagen based cell culture systems having lower oxygen concentration compared to PDMS based cell culture systems. In general, results suggest that oxygen sensing beads can be utilized to achieve real-time and local monitoring of micro-environment oxygen level in 3D microfabricated cell culture systems.

  8. Threshold response using modulated continuous wave illumination for multilayer 3D optical data storage

    NASA Astrophysics Data System (ADS)

    Saini, A.; Christenson, C. W.; Khattab, T. A.; Wang, R.; Twieg, R. J.; Singer, K. D.

    2017-01-01

    In order to achieve a high capacity 3D optical data storage medium, a nonlinear or threshold writing process is necessary to localize data in the axial dimension. To this end, commercial multilayer discs use thermal ablation of metal films or phase change materials to realize such a threshold process. This paper addresses a threshold writing mechanism relevant to recently reported fluorescence-based data storage in dye-doped co-extruded multilayer films. To gain understanding of the essential physics, single layer spun coat films were used so that the data is easily accessible by analytical techniques. Data were written by attenuating the fluorescence using nanosecond-range exposure times from a 488 nm continuous wave laser overlapping with the single photon absorption spectrum. The threshold writing process was studied over a range of exposure times and intensities, and with different fluorescent dyes. It was found that all of the dyes have a common temperature threshold where fluorescence begins to attenuate, and the physical nature of the thermal process was investigated.

  9. Globular and Optically Transparent Photonic Crystals Based on 3D-opal Matrix and REE

    NASA Astrophysics Data System (ADS)

    Ivicheva, S. N.; Kargin, Yu. F.; Gorelik, V. S.

    By repeatedly filling the octahedral and tetrahedral pores of 3D-silica opal matrices with silica sol doped with rare-earth elements with subsequent heat treatment globular photonic crystals filled with mesoporous glass and optically transparent photonic crystals (quantytes) containing 10-30 ppm REE were produced, depending on the annealing temperature. Voids of fcc lattice formed by amorphous spherical globules of SiO2 in globular photonic crystals are filled (up to 70%) by mesoporous glass doped with rare earth elements. Pores in the transparent photonic crystals disappear during sintering of globules of silica and mesoporous glass, but the periodic arrangement of REE-enriched silica areas (quantum dots) is retained. The reflection and luminescence spectra of photonic crystals filled with sols doped with europium Eu3+ and terbium Tb3+ were experimentally studied. A significant increase in the photoluminescence intensity of Eu3+ ions at the approach of the spectral position of the transition 5D0 → 7F2 to the edge of the bandgaps of the photonic crystal was determined. The authors come to the conclusion that a lowering of the threshold for lasing transitions in ions of rare elements is possible.

  10. Large area 3-D optical coherence tomography imaging of lumpectomy specimens for radiation treatment planning

    NASA Astrophysics Data System (ADS)

    Wang, Cuihuan; Kim, Leonard; Barnard, Nicola; Khan, Atif; Pierce, Mark C.

    2016-02-01

    Our long term goal is to develop a high-resolution imaging method for comprehensive assessment of tissue removed during lumpectomy procedures. By identifying regions of high-grade disease within the excised specimen, we aim to develop patient-specific post-operative radiation treatment regimens. We have assembled a benchtop spectral-domain optical coherence tomography (SD-OCT) system with 1320 nm center wavelength. Automated beam scanning enables "sub-volumes" spanning 5 mm x 5 mm x 2 mm (500 A-lines x 500 B-scans x 2 mm in depth) to be collected in under 15 seconds. A motorized sample positioning stage enables multiple sub-volumes to be acquired across an entire tissue specimen. Sub-volumes are rendered from individual B-scans in 3D Slicer software and en face (XY) images are extracted at specific depths. These images are then tiled together using MosaicJ software to produce a large area en face view (up to 40 mm x 25 mm). After OCT imaging, specimens were sectioned and stained with HE, allowing comparison between OCT image features and disease markers on histopathology. This manuscript describes the technical aspects of image acquisition and reconstruction, and reports initial qualitative comparison between large area en face OCT images and HE stained tissue sections. Future goals include developing image reconstruction algorithms for mapping an entire sample, and registering OCT image volumes with clinical CT and MRI images for post-operative treatment planning.

  11. Real Time Gabor-Domain Optical Coherence Microscopy for 3D Imaging.

    PubMed

    Rolland, Jannick P; Canavesi, Cristina; Tankam, Patrice; Cogliati, Andrea; Lanis, Mara; Santhanam, Anand P

    2016-01-01

    Fast, robust, nondestructive 3D imaging is needed for the characterization of microscopic tissue structures across various clinical applications. A custom microelectromechanical system (MEMS)-based 2D scanner was developed to achieve, together with a multi-level GPU architecture, 55 kHz fast-axis A-scan acquisition in a Gabor-domain optical coherence microscopy (GD-OCM) custom instrument. GD-OCM yields high-definition micrometer-class volumetric images. A dynamic depth of focusing capability through a bio-inspired liquid lens-based microscope design, as in whales' eyes, was developed to enable the high definition instrument throughout a large field of view of 1 mm3 volume of imaging. Developing this technology is prime to enable integration within the workflow of clinical environments. Imaging at an invariant resolution of 2 μm has been achieved throughout a volume of 1 × 1 × 0.6 mm3, acquired in less than 2 minutes. Volumetric scans of human skin in vivo and an excised human cornea are presented.

  12. Optical analysis of nanoparticles via enhanced backscattering facilitated by 3-D photonic nanojets.

    PubMed

    Li, Xu; Chen, Zhigang; Taflove, Allen; Backman, Vadim

    2005-01-24

    We report the phenomenon of ultra-enhanced backscattering of visible light by nanoparticles facilitated by the 3-D photonic nanojet - a sub-diffraction light beam appearing at the shadow side of a plane-waveilluminated dielectric microsphere. Our rigorous numerical simulations show that backscattering intensity of nanoparticles can be enhanced up to eight orders of magnitude when locating in the nanojet. As a result, the enhanced backscattering from a nanoparticle with diameter on the order of 10 nm is well above the background signal generated by the dielectric microsphere itself. We also report that nanojet-enhanced backscattering is extremely sensitive to the size of the nanoparticle, permitting in principle resolving sub-nanometer size differences using visible light. Finally, we show how the position of a nanoparticle could be determined with subdiffractional accuracy by recording the angular distribution of the backscattered light. These properties of photonic nanojets promise to make this phenomenon a useful tool for optically detecting, differentiating, and sorting nanoparticles.

  13. Optical analysis of nanoparticles via enhanced backscattering facilitated by 3-D photonic nanojets

    NASA Astrophysics Data System (ADS)

    Li, Xu; Chen, Zhigang; Taflove, Allen; Backman, Vadim

    2005-01-01

    We report the phenomenon of ultra-enhanced backscattering of visible light by nanoparticles facilitated by the 3-D photonic nanojet a sub-diffraction light beam appearing at the shadow side of a plane-waveilluminated dielectric microsphere. Our rigorous numerical simulations show that backscattering intensity of nanoparticles can be enhanced up to eight orders of magnitude when locating in the nanojet. As a result, the enhanced backscattering from a nanoparticle with diameter on the order of 10 nm is well above the background signal generated by the dielectric microsphere itself. We also report that nanojet-enhanced backscattering is extremely sensitive to the size of the nanoparticle, permitting in principle resolving sub-nanometer size differences using visible light. Finally, we show how the position of a nanoparticle could be determined with subdiffractional accuracy by recording the angular distribution of the backscattered light. These properties of photonic nanojets promise to make this phenomenon a useful tool for optically detecting, differentiating, and sorting nanoparticles.

  14. Combining 3D optical imaging and dual energy absorptiometry to measure three compositional components.

    PubMed

    Malkov, Serghei; Shepherd, John

    2014-02-17

    We report on the design of the technique combining 3D optical imaging and dual-energy absorptiometry body scanning to estimate local body area compositions of three compartments. Dual-energy attenuation and body shape measures are used together to solve for the three compositional tissue thicknesses: water, lipid, and protein. We designed phantoms with tissue-like properties as our reference standards for calibration purposes. The calibration was created by fitting phantom values using non-linear regression of quadratic and truncated polynomials. Dual-energy measurements were performed on tissue-mimicking phantoms using a bone densitometer unit. The phantoms were made of materials shown to have similar x-ray attenuation properties of the biological compositional compartments. The components for the solid phantom were tested and their high energy/low energy attenuation ratios are in good correspondent to water, lipid, and protein for the densitometer x-ray region. The three-dimensional body shape was reconstructed from the depth maps generated by Microsoft Kinect for Windows. We used open-source Point Cloud Library and freeware software to produce dense point clouds. Accuracy and precision of compositional and thickness measures were calculated. The error contributions due to two modalities were estimated. The preliminary phantom composition and shape measurements are found to demonstrate the feasibility of the method proposed.

  15. Combining 3D optical imaging and dual energy absorptiometry to measure three compositional components

    PubMed Central

    Malkov, Serghei; Shepherd, John

    2014-01-01

    We report on the design of the technique combining 3D optical imaging and dual-energy absorptiometry body scanning to estimate local body area compositions of three compartments. Dual-energy attenuation and body shape measures are used together to solve for the three compositional tissue thicknesses: water, lipid, and protein. We designed phantoms with tissue-like properties as our reference standards for calibration purposes. The calibration was created by fitting phantom values using non-linear regression of quadratic and truncated polynomials. Dual-energy measurements were performed on tissue-mimicking phantoms using a bone densitometer unit. The phantoms were made of materials shown to have similar x-ray attenuation properties of the biological compositional compartments. The components for the solid phantom were tested and their high energy/low energy attenuation ratios are in good correspondent to water, lipid, and protein for the densitometer x-ray region. The three-dimensional body shape was reconstructed from the depth maps generated by Microsoft Kinect for Windows. We used open-source Point Cloud Library and freeware software to produce dense point clouds. Accuracy and precision of compositional and thickness measures were calculated. The error contributions due to two modalities were estimated. The preliminary phantom composition and shape measurements are found to demonstrate the feasibility of the method proposed. PMID:25083118

  16. Combining 3D optical imaging and dual energy absorptiometry to measure three compositional components

    NASA Astrophysics Data System (ADS)

    Malkov, Serghei; Shepherd, John

    2014-02-01

    We report on the design of the technique combining 3D optical imaging and dual-energy absorptiometry body scanning to estimate local body area compositions of three compartments. Dual-energy attenuation and body shape measures are used together to solve for the three compositional tissue thicknesses: water, lipid, and protein. We designed phantoms with tissue-like properties as our reference standards for calibration purposes. The calibration was created by fitting phantom values using non-linear regression of quadratic and truncated polynomials. Dual-energy measurements were performed on tissue-mimicking phantoms using a bone densitometer unit. The phantoms were made of materials shown to have similar x-ray attenuation properties of the biological compositional compartments. The components for the solid phantom were tested and their high energy/low energy attenuation ratios are in good correspondent to water, lipid, and protein for the densitometer x-ray region. The three-dimensional body shape was reconstructed from the depth maps generated by Microsoft Kinect for Windows. We used open-source Point Cloud Library and freeware software to produce dense point clouds. Accuracy and precision of compositional and thickness measures were calculated. The error contributions due to two modalities were estimated. The preliminary phantom composition and shape measurements are found to demonstrate the feasibility of the method proposed.

  17. 3D Ag/ZnO hybrids for sensitive surface-enhanced Raman scattering detection

    NASA Astrophysics Data System (ADS)

    Huang, Chenyue; Xu, Chunxiang; Lu, Junfeng; Li, Zhaohui; Tian, Zhengshan

    2016-03-01

    To combine the surface plasma resonance of metal and local field enhancement in metal/semiconductor interface, Ag nanoparticles (NPs) were assembled on a ZnO nanorod array which was grown by hydrothermally on carbon fibers. The construction of dimensional (3D) Surface-Enhanced Raman Scattering (SERS) substrate is used for the sensitive detection of organic pollutants with the advantages such as facile synthesis, short detection time and low cost. The hybrid substrate was manifested a high sensitivity to phenol red at a lower concentration of 1 × 10-9 M and a higher enhancement factor of 3.18 × 109. Moreover, the ZnO nanostructures decorated with Ag NPs were demonstrated self-cleaning function under UV irradiation via photocatalytic degradation of the analytic molecules. The fabrication process of the materials and sensors, optimization of the SERS behaviors for different sized Ag NPs, the mechanism of SERS and recovery were presented with a detailed discussion.

  18. Effects of 3D Toroidally Asymmetric Magnetic Field on Tokamak Magnetic Surfaces

    NASA Astrophysics Data System (ADS)

    Lao, L. L.

    2005-10-01

    The effects of 3D error magnetic field on magnetic surfaces are investigated using the DIII-D internal coils (I-Coils). Slowly rotating n=1 traveling waves at 5 Hz and various amplitudes were applied to systematically perturb the edge surfaces by programming the I-Coil currents. The vertical separatrix location difference between EFIT magnetic reconstructions that assumes toroidal symmetry and Thomson scattering Te measurements responds in phase to the applied perturbed field. The oscillation amplitudes increase with the strength of the applied field but are much smaller than those expected from the applied field alone. The results indicate that plasma response is important. Various plasma response models based on results from the MHD codes MARS and GATO are being developed and compared to the experimental observations. To more accurately evaluate the effects of magnetic measurement errors, a new form of the magnetic uncertainty matrix is also being implemented into EFIT. Details will be presented.

  19. GlaRe, a GIS tool to reconstruct the 3D surface of palaeoglaciers

    NASA Astrophysics Data System (ADS)

    Pellitero, Ramón; Rea, Brice R.; Spagnolo, Matteo; Bakke, Jostein; Ivy-Ochs, Susan; Frew, Craig R.; Hughes, Philip; Ribolini, Adriano; Lukas, Sven; Renssen, Hans

    2016-09-01

    Glacier reconstructions are widely used in palaeoclimatic studies and this paper presents a new semi-automated method for generating glacier reconstructions: GlaRe, is a toolbox coded in Python and operating in ArcGIS. This toolbox provides tools to generate the ice thickness from the bed topography along a palaeoglacier flowline applying the standard flow law for ice, and generates the 3D surface of the palaeoglacier using multiple interpolation methods. The toolbox performance has been evaluated using two extant glaciers, an icefield and a cirque/valley glacier from which the subglacial topography is known, using the basic reconstruction routine in GlaRe. Results in terms of ice surface, ice extent and equilibrium line altitude show excellent agreement that confirms the robustness of this procedure in the reconstruction of palaeoglaciers from glacial landforms such as frontal moraines.

  20. Uncertainty studies of topographical measurements on steel surface corrosion by 3D scanning electron microscopy.

    PubMed

    Kang, K W; Pereda, M D; Canafoglia, M E; Bilmes, P; Llorente, C; Bonetto, R

    2012-02-01

    Pitting corrosion is a damage mechanism quite serious and dangerous in both carbon steel boiler tubes for power plants which are vital to most industries and stainless steels for orthopedic human implants whose demand, due to the increase of life expectation and rate of traffic accidents, has sharply increased. Reliable methods to characterize this kind of damage are becoming increasingly necessary, when trying to evaluate the advance of damage and to establish the best procedures for component inspection in order to determine remaining lives and failure mitigation. A study about the uncertainties on the topographies of corrosion pits from 3D SEM images, obtained at low magnifications (where errors are greater) and different stage tilt angles were carried out using an in-house software previously developed. Additionally, measurements of pit depths on biomaterial surfaces, subjected to two different surface treatments on stainless steels, were carried out. The different depth distributions observed were in agreement with electrochemical measurements.

  1. Contact Interface Verification for DYNA3D Scenario 2: Multi-Surface Contact

    SciTech Connect

    McMichael, L D

    2006-05-10

    A suite of test problems has been developed to examine contact behavior within the nonlinear, three-dimensional, explicit finite element analysis (FEA) code DYNA3D (Lin, 2005). The test problems use multiple interfaces and a combination of enforcement methods to assess the basic functionality of the contact algorithms. The results from the DYNA3D analyses are compared to closed form solutions to verify the contact behavior. This work was performed as part of the Verification and Validation efforts of LLNL W Program within the NNSA's Advanced Simulation and Computing (ASC) Program. DYNA3D models the transient dynamic response of solids and structures including the interactions between disjoint bodies (parts). A wide variety of contact surfaces are available to represent the diverse interactions possible during an analysis, including relative motion (sliding), separation and gap closure (voids), and fixed relative position (tied). The problem geometry may be defined using a combination of element formulations, including one-dimensional beam and truss elements, two-dimensional shell elements, and three-dimensional solid elements. Consequently, it is necessary to consider various element interactions during contact. This report and associated test problems examine the scenario where multiple bodies interact with each other via multiple interfaces. The test problems focus on whether any ordering issues exist in the contact logic by using a combination of interface types, contact enforcement options (i.e., penalty, Lagrange, and kinematic), and element interactions within each problem. The influence of rigid materials on interface behavior is also examined. The companion report (McMichael, 2006) and associated test problems address the basic contact scenario where one contact surface exists between two disjoint bodies. The test problems are analyzed using version 5.2 (compiled on 12/22/2005) of DYNA3D. The analytical results are used to form baseline solutions for

  2. Non-Newtonian Fluids Spreading with Surface Tension Effect: 3D Numerical Analysis Using FEM and Experimental Study

    NASA Astrophysics Data System (ADS)

    Hu, Bin; Kieweg, Sarah

    2010-11-01

    Gravity-driven thin film flow down an incline is studied for optimal design of polymeric drug delivery vehicles, such as anti-HIV topical microbicides. We develop a 3D FEM model using non-Newtonian mechanics to model the flow of gels in response to gravity, surface tension and shear-thinning. Constant volume setup is applied within the lubrication approximation scope. The lengthwise profiles of the 3D model agree with our previous 2D finite difference model, while the transverse contact line patterns of the 3D model are compared to the experiments. With incorporation of surface tension, capillary ridges are observed at the leading front in both 2D and 3D models. Previously published studies show that capillary ridge can amplify the fingering instabilities in transverse direction. Sensitivity studies (2D & 3D) and experiments are carried out to describe the influence of surface tension and shear-thinning on capillary ridge and fingering instabilities.

  3. Construction of Extended 3D Field of Views of the Internal Bladder Wall Surface: A Proof of Concept

    NASA Astrophysics Data System (ADS)

    Ben-Hamadou, Achraf; Daul, Christian; Soussen, Charles

    2016-09-01

    3D extended field of views (FOVs) of the internal bladder wall facilitate lesion diagnosis, patient follow-up and treatment traceability. In this paper, we propose a 3D image mosaicing algorithm guided by 2D cystoscopic video-image registration for obtaining textured FOV mosaics. In this feasibility study, the registration makes use of data from a 3D cystoscope prototype providing, in addition to each small FOV image, some 3D points located on the surface. This proof of concept shows that textured surfaces can be constructed with minimally modified cystoscopes. The potential of the method is demonstrated on numerical and real phantoms reproducing various surface shapes. Pig and human bladder textures are superimposed on phantoms with known shape and dimensions. These data allow for quantitative assessment of the 3D mosaicing algorithm based on the registration of images simulating bladder textures.

  4. Region-Based 3d Surface Reconstruction Using Images Acquired by Low-Cost Unmanned Aerial Systems

    NASA Astrophysics Data System (ADS)

    Lari, Z.; Al-Rawabdeh, A.; He, F.; Habib, A.; El-Sheimy, N.

    2015-08-01

    Accurate 3D surface reconstruction of our environment has become essential for an unlimited number of emerging applications. In the past few years, Unmanned Aerial Systems (UAS) are evolving as low-cost and flexible platforms for geospatial data collection that could meet the needs of aforementioned application and overcome limitations of traditional airborne and terrestrial mobile mapping systems. Due to their payload restrictions, these systems usually include consumer-grade imaging and positioning sensor which will negatively impact the quality of the collected geospatial data and reconstructed surfaces. Therefore, new surface reconstruction surfaces are needed to mitigate the impact of using low-cost sensors on the final products. To date, different approaches have been proposed to for 3D surface construction using overlapping images collected by imaging sensor mounted on moving platforms. In these approaches, 3D surfaces are mainly reconstructed based on dense matching techniques. However, generated 3D point clouds might not accurately represent the scanned surfaces due to point density variations and edge preservation problems. In order to resolve these problems, a new region-based 3D surface renostruction trchnique is introduced in this paper. This approach aims to generate a 3D photo-realistic model of individually scanned surfaces within the captured images. This approach is initiated by a Semi-Global dense Matching procedure is carried out to generate a 3D point cloud from the scanned area within the collected images. The generated point cloud is then segmented to extract individual planar surfaces. Finally, a novel region-based texturing technique is implemented for photorealistic reconstruction of the extracted planar surfaces. Experimental results using images collected by a camera mounted on a low-cost UAS demonstrate the feasibility of the proposed approach for photorealistic 3D surface reconstruction.

  5. Surface functionalization of 3D glass-ceramic porous scaffolds for enhanced mineralization in vitro

    NASA Astrophysics Data System (ADS)

    Ferraris, Sara; Vitale-Brovarone, Chiara; Bretcanu, Oana; Cassinelli, Clara; Vernè, Enrica

    2013-04-01

    Bone reconstruction after tissue loosening due to traumatic, pathological or surgical causes is in increasing demand. 3D scaffolds are a widely studied solution for supporting new bone growth. Bioactive glass-ceramic porous materials can offer a three-dimensional structure that is able to chemically bond to bone. The ability to surface modify these devices by grafting biologically active molecules represents a challenge, with the aim of stimulating physiological bone regeneration with both inorganic and organic signals. In this research work glass ceramic scaffolds with very high mechanical properties and moderate bioactivity have been functionalized with the enzyme alkaline phosphatase (ALP). The material surface was activated in order to expose hydroxyl groups. The activated surface was further grafted with ALP both via silanization and also via direct grafting to the surface active hydroxyl groups. Enzymatic activity of grafted samples were measured by means of UV-vis spectroscopy before and after ultrasonic washing in TRIS-HCl buffer solution. In vitro inorganic bioactivity was investigated by soaking the scaffolds after the different steps of functionalization in a simulated body fluid (SBF). SEM observations allowed the monitoring of the scaffold morphology and surface chemical composition after soaking in SBF. The presence of ALP enhanced the in vitro inorganic bioactivity of the tested material.

  6. 3D Imaging of Water-Drop Condensation on Hydrophobic and Hydrophilic Lubricant-Impregnated Surfaces

    PubMed Central

    Kajiya, Tadashi; Schellenberger, Frank; Papadopoulos, Periklis; Vollmer, Doris; Butt, Hans-Jürgen

    2016-01-01

    Condensation of water from the atmosphere on a solid surface is an ubiquitous phenomenon in nature and has diverse technological applications, e.g. in heat and mass transfer. We investigated the condensation kinetics of water drops on a lubricant-impregnated surface, i.e., a micropillar array impregnated with a non-volatile ionic liquid. Growing and coalescing drops were imaged in 3D using a laser scanning confocal microscope equipped with a temperature and humidity control. Different stages of condensation can be discriminated. On a lubricant-impregnated hydrophobic micropillar array these are: (1) Nucleation on the lubricant surface. (2) Regular alignment of water drops between micropillars and formation of a three-phase contact line on a bottom of the substrate. (3) Deformation and bridging by coalescence which eventually leads to a detachment of the drops from the bottom substrate. The drop-substrate contact does not result in breakdown of the slippery behaviour. Contrary, on a lubricant-impregnated hydrophilic micropillar array, the condensed water drops replace the lubricant. Consequently, the surface loses its slippery property. Our results demonstrate that a Wenzel-like to Cassie transition, required to maintain the facile removal of condensed water drops, can be induced by well-chosen surface hydrophobicity. PMID:27040483

  7. 3D Imaging of Water-Drop Condensation on Hydrophobic and Hydrophilic Lubricant-Impregnated Surfaces

    NASA Astrophysics Data System (ADS)

    Kajiya, Tadashi; Schellenberger, Frank; Papadopoulos, Periklis; Vollmer, Doris; Butt, Hans-Jürgen

    2016-04-01

    Condensation of water from the atmosphere on a solid surface is an ubiquitous phenomenon in nature and has diverse technological applications, e.g. in heat and mass transfer. We investigated the condensation kinetics of water drops on a lubricant-impregnated surface, i.e., a micropillar array impregnated with a non-volatile ionic liquid. Growing and coalescing drops were imaged in 3D using a laser scanning confocal microscope equipped with a temperature and humidity control. Different stages of condensation can be discriminated. On a lubricant-impregnated hydrophobic micropillar array these are: (1) Nucleation on the lubricant surface. (2) Regular alignment of water drops between micropillars and formation of a three-phase contact line on a bottom of the substrate. (3) Deformation and bridging by coalescence which eventually leads to a detachment of the drops from the bottom substrate. The drop-substrate contact does not result in breakdown of the slippery behaviour. Contrary, on a lubricant-impregnated hydrophilic micropillar array, the condensed water drops replace the lubricant. Consequently, the surface loses its slippery property. Our results demonstrate that a Wenzel-like to Cassie transition, required to maintain the facile removal of condensed water drops, can be induced by well-chosen surface hydrophobicity.

  8. Effects of Surface Morphology on the 3D Topological Insulator Samarium Hexaboride

    NASA Astrophysics Data System (ADS)

    Wolgast, Steven; Eo, Yun Suk; Kurdak, Cagliyan; Kim, Dae-Jeong; Fisk, Zachary

    2015-03-01

    The recent verification of a topologically-protected surface state in SmB6 at low temperatures has led to several transport studies of the surface states. This task is complicated because current can flow on all surfaces of a topological insulator, each of which can have different transport characteristics. Our own measurements using a Corbino disc geometry overcome this difficulty, limiting the conduction to individual surfaces. However, the sheet conductivities of our samples counter-intuitively decrease with finer surface polishing. We therefore investigate surface and sub-surface morphology as a factor affecting the surface conductivity. Specifically, surface cracks may themselves harbor surface states and contribute to the total electrical conduction, yielding a higher measured sheet conductivity than that of a flat surface. This situation may contribute to the (sometimes unphysically) large surface conductivities already observed in SmB6. Performed in part at the Lurie Nanofabrication Facility and the Electron Microbeam Analysis Laboratory. Funded by NSF Grant #DMR-1006500 and DMR-1441965. Thanks to Gang Li and Fan Yu for optical imaging.

  9. 3D Surface Profile and Color Stability of Tooth Colored Filling Materials after Bleaching

    PubMed Central

    Irawan, Bryant Anthony; Irawan, Stacey Natalie; Masudi, Sam'an Malik; Sukminingrum, Ninin; Alam, Mohammad Khursheed

    2015-01-01

    This study aims to evaluate the effects of vital tooth bleaching with carbamide peroxide home bleaching and in-office bleaching on the color stability and 3D surface profile of dental restorative filling materials. Thirty discs (n = 30) measure 6 mm in diameter and 2 mm thick for each of three restorative materials. These are nanofilled composite Filtek Z350 XT, the submicron composite Estelite Σ Quick, and nanofilled glass ionomer Ketac N100 nanoionomer and were fabricated in shade A2. Each group was further divided into three subgroups (n = 10): subgroup A (Opalescence PF), subgroup B (Opalescence Boost in-office bleaching), and subgroup C (distilled water) serving as control. Samples were bleached according to the manufacturer's instructions for a period of two weeks. The Commission Internationale de L'Eclairage (CIE L*, a*, b*) system was chosen for image processing, while 3D surface profile was tested with atomic force microscopy (AFM). Statistical analyses were performed with the Mann-Whitney tests and Krusal-Wallis with a P value of ≤0.05. The three restorative materials showed significant color changes (ΔE); P ≤ 0.05. In diminishing order, the mean color changes recorded were Estelite Σ (3.82 ± 1.6) > Ketac Nano (2.97 ± 1.2) > Filtek Z350 XT (2.25 ± 1.0). However, none of the tested materials showed statistically significant changes in surface roughness; P > 0.05. PMID:26558267

  10. 3D Surface Reconstruction of Rills in a Spanish Olive Grove

    NASA Astrophysics Data System (ADS)

    Brings, Christine; Gronz, Oliver; Seeger, Manuel; Wirtz, Stefan; Taguas, Encarnación; Ries, Johannes B.

    2016-04-01

    The low-cost, user-friendly photogrammetric Structure from Motion (SfM) technique is used for 3D surface reconstruction and difference calculation of an 18 meter long rill in South Spain (Andalusia, Puente Genil). The images were taken with a Canon HD video camera before and after a rill experiment in an olive grove. Recording with a video camera has compared to a photo camera a huge time advantage and the method also guarantees more than adequately overlapping sharp images. For each model, approximately 20 minutes of video were taken. As SfM needs single images, the sharpest image was automatically selected from 8 frame intervals. The sharpness was estimated using a derivative-based metric. Then, VisualSfM detects feature points in each image, searches matching feature points in all image pairs and recovers the camera and feature positions. Finally, by triangulation of camera positions and feature points the software reconstructs a point cloud of the rill surface. From the point cloud, 3D surface models (meshes) are created and via difference calculations of the pre and post model a visualization of the changes (erosion and accumulation areas) and quantification of erosion volumes are possible. The calculated volumes are presented in spatial units of the models and so real values must be converted via references. The results show that rills in olive groves have a high dynamic due to the lack of vegetation cover under the trees, so that the rill can incise until the bedrock. Another reason for the high activity is the intensive employment of machinery.

  11. 3D nano surface profilometry by combining the photonic nanojet with interferometry

    NASA Astrophysics Data System (ADS)

    Montgomery, P. C.; Lecler, S.; Leong-Hoï, A.; Pfeiffer, P.

    2017-01-01

    Interference microscopy has become a standard technique for measuring microscopic surface roughness, being rapid, non-contact and having a high nanometric axial resolution. Nonetheless, a limitation is the lateral resolution of λ/2 due to diffraction. Amongst the many recently developed techniques for improving the lateral resolution in unlabelled far field imaging, the use of microspheres placed on the sample has recently been extended from 2D imaging to 3D measurement by combining it with interferometry. In the present work, results are shown of combining the high lateral resolution of the photonic nanojet produced by the microsphere with interference microscopy to achieve sub-diffraction limited lateral resolution with nanometric axial resolution. This is achieved by imaging through a microsphere placed on top of the sample in front of the interference objective in a Linnik setup. Results of the 3D measurements of narrow gratings through the microsphere are presented, that are not observable with the same objective in air since they are below the resolution limit. Simulations of the interaction between the photonic jet and the grating leads to a basic understanding of the image formation. This new technique opens new possibilities for high resolution characterization in nanomaterials and the biological sciences.

  12. 3D Simulation of Missing Pellet Surface Defects in Light Water Reactor Fuel Rods

    SciTech Connect

    B.W. Spencer; J.D. Hales; S.R. Novascone; R.L. Williamson

    2012-09-01

    The cladding on light water reactor (LWR) fuel rods provides a stable enclosure for fuel pellets and serves as a first barrier against fission product release. Consequently, it is important to design fuel to prevent cladding failure due to mechanical interactions with fuel pellets. Cladding stresses can be effectively limited by controlling power increase rates. However, it has been shown that local geometric irregularities caused by manufacturing defects known as missing pellet surfaces (MPS) in fuel pellets can lead to elevated cladding stresses that are sufficiently high to cause cladding failure. Accurate modeling of these defects can help prevent these types of failures. Nuclear fuel performance codes commonly use a 1.5D (axisymmetric, axially-stacked, one-dimensional radial) or 2D axisymmetric representation of the fuel rod. To study the effects of MPS defects, results from 1.5D or 2D fuel performance analyses are typically mapped to thermo-mechanical models that consist of a 2D plane-strain slice or a full 3D representation of the geometry of the pellet and clad in the region of the defect. The BISON fuel performance code developed at Idaho National Laboratory employs either a 2D axisymmetric or 3D representation of the full fuel rod. This allows for a computational model of the full fuel rod to include local defects. A 3D thermo-mechanical model is used to simulate the global fuel rod behavior, and includes effects on the thermal and mechanical behavior of the fuel due to accumulation of fission products, fission gas production and release, and the effects of fission gas accumulation on thermal conductivity across the fuel-clad gap. Local defects can be modeled simply by including them in the 3D fuel rod model, without the need for mapping between two separate models. This allows for the complete set of physics used in a fuel performance analysis to be included naturally in the computational representation of the local defect, and for the effects of the

  13. Surface feature based classification of plant organs from 3D laserscanned point clouds for plant phenotyping

    PubMed Central

    2013-01-01

    Background Laserscanning recently has become a powerful and common method for plant parameterization and plant growth observation on nearly every scale range. However, 3D measurements with high accuracy, spatial resolution and speed result in a multitude of points that require processing and analysis. The primary objective of this research has been to establish a reliable and fast technique for high throughput phenotyping using differentiation, segmentation and classification of single plants by a fully automated system. In this report, we introduce a technique for automated classification of point clouds of plants and present the applicability for plant parameterization. Results A surface feature histogram based approach from the field of robotics was adapted to close-up laserscans of plants. Local geometric point features describe class characteristics, which were used to distinguish among different plant organs. This approach has been proven and tested on several plant species. Grapevine stems and leaves were classified with an accuracy of up to 98%. The proposed method was successfully transferred to 3D-laserscans of wheat plants for yield estimation. Wheat ears were separated with an accuracy of 96% from other plant organs. Subsequently, the ear volume was calculated and correlated to the ear weight, the kernel weights and the number of kernels. Furthermore the impact of the data resolution was evaluated considering point to point distances between 0.3 and 4.0 mm with respect to the classification accuracy. Conclusion We introduced an approach using surface feature histograms for automated plant organ parameterization. Highly reliable classification results of about 96% for the separation of grapevine and wheat organs have been obtained. This approach was found to be independent of the point to point distance and applicable to multiple plant species. Its reliability, flexibility and its high order of automation make this method well suited for the demands of

  14. Comparison of two different surfaces for 3d model abstraction in support of remote sensing simulations

    SciTech Connect

    Pope, Paul A; Ranken, Doug M

    2010-01-01

    A method for abstracting a 3D model by shrinking a triangular mesh, defined upon a best fitting ellipsoid surrounding the model, onto the model's surface has been previously described. This ''shrinkwrap'' process enables a semi-regular mesh to be defined upon an object's surface. This creates a useful data structure for conducting remote sensing simulations and image processing. However, using a best fitting ellipsoid having a graticule-based tessellation to seed the shrinkwrap process suffers from a mesh which is too dense at the poles. To achieve a more regular mesh, the use of a best fitting, subdivided icosahedron was tested. By subdividing each of the twenty facets of the icosahedron into regular triangles of a predetermined size, arbitrarily dense, highly-regular starting meshes can be created. Comparisons of the meshes resulting from these two seed surfaces are described. Use of a best fitting icosahedron-based mesh as the seed surface in the shrinkwrap process is preferable to using a best fitting ellipsoid. The impacts to remote sensing simulations, specifically generation of synthetic imagery, is illustrated.

  15. Key parameters in blood-surface interactions of 3D bioinspired ceramic materials.

    PubMed

    Díaz-Rodríguez, P; González, P; Serra, J; Landin, M

    2014-08-01

    Direct contact of materials with blood components may trigger numerous processes which ultimately lead to hemolysis, clot formation and recruitment of inflammatory cells. In this study, the blood-surface interactions for two inert bioinspired ceramic scaffolds obtained from natural resources; biomorphic carbon and silicon carbides (bioSiC) from different origins have been studied. The response of the blood in contact with carbon is well known, however little has been identified on the influence of their 3D porous structure. Moreover, to our knowledge, there is no reference in the literature about the hemocompatibility of biomorphic silicon carbide as a porous scaffold. The experimental results showed the surface energy to be crucial to evaluate the hemocompatibility of a material however the surface topography and material porosity are also parameters to be considered. Surface roughness modifies clot formation whereas for protein adsorption total sample porosity seems to be the key parameter to be considered for hydrophilic materials (biomorphic silicon carbides), while the size of the pores determines the hemolytic response.

  16. Creation of quantum-degenerate gases of ytterbium in a compact 2D-/3D-magneto-optical trap setup

    SciTech Connect

    Doerscher, Soeren; Thobe, Alexander; Hundt, Bastian; Kochanke, Andre; Le Targat, Rodolphe; Windpassinger, Patrick; Becker, Christoph; Sengstock, Klaus

    2013-04-15

    We report on the first experimental setup based on a 2D-/3D-magneto-optical trap (MOT) scheme to create both Bose-Einstein condensates and degenerate Fermi gases of several ytterbium isotopes. Our setup does not require a Zeeman slower and offers the flexibility to simultaneously produce ultracold samples of other atomic species. Furthermore, the extraordinary optical access favors future experiments in optical lattices. A 2D-MOT on the strong {sup 1}S{sub 0}{yields}{sup 1}P{sub 1} transition captures ytterbium directly from a dispenser of atoms and loads a 3D-MOT on the narrow {sup 1}S{sub 0}{yields}{sup 3}P{sub 1} intercombination transition. Subsequently, atoms are transferred to a crossed optical dipole trap and cooled evaporatively to quantum degeneracy.

  17. Investigation of 3D surface acoustic waves in granular media with 3-color digital holography

    NASA Astrophysics Data System (ADS)

    Leclercq, Mathieu; Picart, Pascal; Penelet, Guillaume; Tournat, Vincent

    2017-01-01

    This paper reports the implementation of digital color holography to investigate elastic waves propagating along a layer of a granular medium. The holographic set-up provides simultaneous recording and measurement of the 3D dynamic displacement at the surface. Full-field measurements of the acoustic amplitude and phase at different excitation frequencies are obtained. It is shown that the experimental data can be used to obtain the dispersion curve of the modes propagating in this granular medium layer. The experimental dispersion curve and that obtained from a finite element modeling of the problem are found to be in good agreement. In addition, full-field images of the interaction of an acoustic wave guided in the granular layer with a buried object are also shown.

  18. 3-D surface profilometry based on modulation measurement by applying wavelet transform method

    NASA Astrophysics Data System (ADS)

    Zhong, Min; Chen, Feng; Xiao, Chao; Wei, Yongchao

    2017-01-01

    A new analysis of 3-D surface profilometry based on modulation measurement technique by the application of Wavelet Transform method is proposed. As a tool excelling for its multi-resolution and localization in the time and frequency domains, Wavelet Transform method with good localized time-frequency analysis ability and effective de-noizing capacity can extract the modulation distribution more accurately than Fourier Transform method. Especially for the analysis of complex object, more details of the measured object can be well remained. In this paper, the theoretical derivation of Wavelet Transform method that obtains the modulation values from a captured fringe pattern is given. Both computer simulation and elementary experiment are used to show the validity of the proposed method by making a comparison with the results of Fourier Transform method. The results show that the Wavelet Transform method has a better performance than the Fourier Transform method in modulation values retrieval.

  19. Design of a 3D surface scanner prototype suitable for MR.

    PubMed

    Cavalleri, M; Romei, M; Reni, G

    2010-01-01

    The detection of body movements during MR examination could help in reducing motion artifacts or to get patient responses during functional magnetic resonance. It can be supported by a slit scanner, that combines a camera with a light stripe projector to obtain 3D coordinates of points forming the external surface of the body. In this work we propose a slit scanner prototype based on a miniaturized projector without moving parts. Just small sized hardware is required to analyze the video signal, operating in time domain instead of spatial domain. To accomplish this, the camera is placed with its pixel columns as more parallel as possible to the projected light stripes and the camera video signal is analyzed by a resistor transistor logic after analog processing.

  20. Automatic extraction of discontinuity orientation from rock mass surface 3D point cloud

    NASA Astrophysics Data System (ADS)

    Chen, Jianqin; Zhu, Hehua; Li, Xiaojun

    2016-10-01

    This paper presents a new method for extracting discontinuity orientation automatically from rock mass surface 3D point cloud. The proposed method consists of four steps: (1) automatic grouping of discontinuity sets using an improved K-means clustering method, (2) discontinuity segmentation and optimization, (3) discontinuity plane fitting using Random Sample Consensus (RANSAC) method, and (4) coordinate transformation of discontinuity plane. The method is first validated by the point cloud of a small piece of a rock slope acquired by photogrammetry. The extracted discontinuity orientations are compared with measured ones in the field. Then it is applied to a publicly available LiDAR data of a road cut rock slope at Rockbench repository. The extracted discontinuity orientations are compared with the method proposed by Riquelme et al. (2014). The results show that the presented method is reliable and of high accuracy, and can meet the engineering needs.

  1. UAV based 3D digital surface model to estimate paleolandscape in high mountainous environment

    NASA Astrophysics Data System (ADS)

    Mészáros, János; Árvai, Mátyás; Kohán, Balázs; Deák, Márton; Nagy, Balázs

    2016-04-01

    Our method to present current state of a peat bog was focused on the possible use of a UAV-system and later Structure-from-motion algorithms as processing technique. The peat bog site is located on the Vinderel Plateau, Farcǎu Massif, Maramures Mountains (Romania). The peat bog (1530 m a.s.l., N47°54'11", E24°26'37") lies below Rugasu ridge (c. 1820 m a.s.l.) and the locality serves as a conservation area for fallen down coniferous trees. Peat deposits were formed in a landslide concavity on the western slope of Farcǎu Massif. Nowadays the site is surrounded by a completely deforested landscape, and Farcǎu Massif lies above the depressed treeline. The peat bog has an extraordinary geomorphological situation, because a gully reached the bog and drained the water. In the recent past sedimentological and dendrochronological researches have been initiated. However, an accurate 3D digital surface model also needed for a complex paleoenvironmental research. Last autumn the bog and its surroundings were finally surveyed by a multirotor UAV developed in-house based on an open-source flight management unit and its firmware. During this survey a lightweight action camera (mainly to decrease payload weight) was used to take aerial photographs. While our quadcopter is capable to fly automatically on a predefined flight route, several over- and sidelapping flight lines were generated prior to the actual survey on the ground using a control software running on a notebook. Despite those precautions, limited number of batteries and severe weather affected our final flights, resulting a reduced surveyed area around peat bog. Later, during the processing we looked for a reliable tool which powerful enough to process more than 500 photos taken during flights. After testing several software Agisoft PhotoScan was used to create 3D point cloud and mesh about bog and its environment. Due to large number of photographs PhotoScan had to be configured for network processing to get

  2. Optical oscillator strengths for valence-shell and Br-3d inner-shell excitations of HCl and HBr.

    PubMed

    Li, Wen-Bin; Zhu, Lin-Fan; Yuan, Zhen-Sheng; Liu, Xiao-Jing; Xu, Ke-Zun

    2006-10-21

    Absolute optical oscillator strength density spectra for valence-shell excitations of HCl and HBr, as well as for Br-3d inner-shell excitations of HBr, have been determined by high-resolution electron-energy-loss-spectroscopy method in the dipole limit. Absolute optical oscillator strengths for the discrete transitions of HCl and HBr are reported and compared with the previous results determined by the photoabsorption method.

  3. Understanding surface processes 3D imaging from micro-scale to regional scale

    NASA Astrophysics Data System (ADS)

    Jaboyedoff, Michel; Abellan, Antonio; Carrea, Dario; Derron, Marc-Henri; Franz, Martin; Guerin, Antoine; Humair, Florian; Matasci, Battista; Michoud, Clément; Nicolet, Pierrick; Penna, Ivanna; Rudaz, Benjamin; Voumard, Jeremie; Wyser, Emmanuel

    2015-04-01

    The production of topography using remote sensing techniques has considerably been improved during the last fifteen years due to the advances in electronics and to the increase of computing power. The earth surface is monitored at all the scales using Space Shuttle Missions (SRTM) digital elevation model (DEM), or using laser scanner (LS), both terrestrial (TLS) and airborne (ALS), with accuracies that can reach up to less than 50 microns for observations of objects at meter scale. Recently, photogrammetry has been pushed by the progress of LiDAR and thanks to the advance in image recognition. It led to the development of new techniques such as structure-from-motion (SFM), which allows obtaining 3D point cloud based on several pictures of the same object taken from several point of views. Both LiDAR and Photogrammetry produce 3D point clouds. One of the current 3D applications is the surface changes, which is often based simply on the subtraction of DEM at different time intervals, leading to a simple superficial description of the natural processes without information on the mass transport. However, a point cloud has much more information than a simple surface. For instance, shape recognition can be used to track objects or deformations such as a rock mass toppling, either using the shape of the point cloud or a specific moving element. Such method permits, for instance, to study in detail pre-failure accelerations, and are now routinely used in mining industry. Other methods are coupling images and DEMs and are used, for example, to capture the surface vectors of displacements in order to deduce the surface deformations of landslides. These types of surveys have now broad applications to all kinds of erosional processes. The coastal retreat can be monitored, and it displays in some places several centimetres per year of retreat on average. The sediment transports in torrent are now better constraint showing clearly pulses. The seasonal cycles can as well be

  4. 3D Cloud Tomography, Followed by Mean Optical and Microphysical Properties, with Multi-Angle/Multi-Pixel Data

    NASA Astrophysics Data System (ADS)

    Davis, A. B.; von Allmen, P. A.; Marshak, A.; Bal, G.

    2010-12-01

    -type model is used where the cloud surface "emits" either reflected (sunny-side) or transmitted (shady-side) light at different levels. As it turns out, the reflected/transmitted light ratio yields an approximate cloud optical thickness. Another approach is to invoke tomography techniques to define the volume occupied by the cloud using, as it were, cloud masks for each direction of observation. In the shape and opacity refinement phase, initial guesses along with solar and viewing geometry information are used to predict radiance in each pixel using a fast diffusion model for the 3D RT in MISR's non-absorbing red channel (275 m resolution). Refinement is constrained and stopped when optimal resolution is reached. Finally, multi-pixel/mono-angle MODIS data for the same cloud (at comparable 250 m resolution) reveals the desired droplet size information, hence the volume-averaged LWC. This is an ambitious remote sensing science project drawing on cross-disciplinary expertise gained in medical imaging using both X-ray and near-IR sources and detectors. It is high risk but with potentially high returns not only for the cloud modeling community but also aerosol and surface characterization in the presence of broken 3D clouds.

  5. Analyzing the Evolution of Membrane Fouling via a Novel Method Based on 3D Optical Coherence Tomography Imaging.

    PubMed

    Li, Weiyi; Liu, Xin; Wang, Yi-Ning; Chong, Tzyy Haur; Tang, Chuyang Y; Fane, Anthony G

    2016-07-05

    The development of novel tools for studying the fouling behavior during membrane processes is critical. This work explored optical coherence tomography (OCT) to quantitatively interpret the formation of a cake layer during a membrane process; the quantitative analysis was based on a novel image processing method that was able to precisely resolve the 3D structure of the cake layer on a micrometer scale. Fouling experiments were carried out with foulants having different physicochemical characteristics (silica nanoparticles and bentonite particles). The cake layers formed at a series of times were digitalized using the OCT-based characterization. The specific deposit (cake volume/membrane surface area) and surface coverage were evaluated as a function of time, which for the first time provided direct experimental evidence for the transition of various fouling mechanisms. Axial stripes were observed in the grayscale plots showing the deposit distribution in the scanned area; this interesting observation was in agreement with the instability analysis that correlated the polarized particle groups with the small disturbances in the boundary layer. This work confirms that the OCT-based characterization is able to provide deep insights into membrane fouling processes and offers a powerful tool for exploring membrane processes with enhanced performance.

  6. 3D shape reconstruction of specular surfaces by using phase measuring deflectometry

    NASA Astrophysics Data System (ADS)

    Zhou, Tian; Chen, Kun; Wei, Haoyun; Li, Yan

    2016-10-01

    The existing estimation methods for recovering height information from surface gradient are mainly divided into Modal and Zonal techniques. Since specular surfaces used in the industry always have complex and large areas, considerations must be given to both the improvement of measurement accuracy and the acceleration of on-line processing speed, which beyond the capacity of existing estimations. Incorporating the Modal and Zonal approaches into a unifying scheme, we introduce an improved 3D shape reconstruction version of specular surfaces based on Phase Measuring Deflectometry in this paper. The Modal estimation is firstly implemented to derive the coarse height information of the measured surface as initial iteration values. Then the real shape can be recovered utilizing a modified Zonal wave-front reconstruction algorithm. By combining the advantages of Modal and Zonal estimations, the proposed method simultaneously achieves consistently high accuracy and dramatically rapid convergence. Moreover, the iterative process based on an advanced successive overrelaxation technique shows a consistent rejection of measurement errors, guaranteeing the stability and robustness in practical applications. Both simulation and experimentally measurement demonstrate the validity and efficiency of the proposed improved method. According to the experimental result, the computation time decreases approximately 74.92% in contrast to the Zonal estimation and the surface error is about 6.68 μm with reconstruction points of 391×529 pixels of an experimentally measured sphere mirror. In general, this method can be conducted with fast convergence speed and high accuracy, providing an efficient, stable and real-time approach for the shape reconstruction of specular surfaces in practical situations.

  7. Characterization of 3D printing output using an optical sensing system

    NASA Astrophysics Data System (ADS)

    Straub, Jeremy

    2015-05-01

    This paper presents the experimental design and initial testing of a system to characterize the progress and performance of a 3D printer. The system is based on five Raspberry Pi single-board computers. It collects images of the 3D printed object, which are compared to an ideal model. The system, while suitable for printers of all sizes, can potentially be produced at a sufficiently low cost to allow its incorporation into consumer-grade printers. The efficacy and accuracy of this system is presented and discussed. The paper concludes with a discussion of the benefits of being able to characterize 3D printer performance.

  8. Developed Design for Humeral Head Replacement Using 3D Surface Mapping

    NASA Astrophysics Data System (ADS)

    Salah, H. R.

    2014-12-01

    Assessment of dimensional and geometrical data on the humeral head replacement (HHR) objects is essential for solving the relevant designing problems in the physics of reverse engineering (RE). In this work, 2D-assessment for human humerus was performed using the computed tomography (CT) technique within the RE plan, after which the 2D images of humeral objects were converted into 3D images. The conversion was successful and indicated a clear difference in the 2D and 3D estimates of sizes and geometry of the humerus. The authors have analyzed and confirmed experimentally the statistical information on the relevant anatomical objects. The results of finite-element simulation of the compressive stresses affecting the geometry of 3D surface mapping were analyzed using SolidWorks software. For developing the biomechanical design of an HHR object suitable biomaterials were selected, and different metal-based biomaterials are discussed as applied at various loads. New methodology is presented for the size estimation of humeral head - both anatomical and artificial - in 3D-shape. A detailed interpretation is given for the results of CT D-measurements. Izmēru un ģeometrisko datu novērtējums, kas attiecas uz pleca kaula galviņas nomaiņas (PKGN) objektiem, nepieciešams, lai risinātu virkni reversīvās inženierijas (RI) problēmu. Šajā darbā cilvēka pleca kaula galviņas divdimensiju novērtējums tika veikts ar datortomogrāfijas palīdzību (RI) ietvaros, un pēc tam objekta divdimensiju attēlojums tika pārveidots trīsdimensiju. Pārveidojums bija sekmīgs, parādot pleca kaula galviņas izmēru un ģeometrijas atšķirības starp 2D un 3D novērtējumiem. Autori izanalizēja un eksperimentāli apstiprināja statistisko informāciju pēc dotā veida anatomiskiem objektiem. Saspiešanas sasprindzinājumi, kuri ietekmē trīsdimensiju virsmas attēlojuma ģeometriju, tika analizēti ar gala-elementu simulācijas metodi, lietojot programmu Solid

  9. Evaluating Dense 3d Reconstruction Software Packages for Oblique Monitoring of Crop Canopy Surface

    NASA Astrophysics Data System (ADS)

    Brocks, S.; Bareth, G.

    2016-06-01

    Crop Surface Models (CSMs) are 2.5D raster surfaces representing absolute plant canopy height. Using multiple CMSs generated from data acquired at multiple time steps, a crop surface monitoring is enabled. This makes it possible to monitor crop growth over time and can be used for monitoring in-field crop growth variability which is useful in the context of high-throughput phenotyping. This study aims to evaluate several software packages for dense 3D reconstruction from multiple overlapping RGB images on field and plot-scale. A summer barley field experiment located at the Campus Klein-Altendorf of University of Bonn was observed by acquiring stereo images from an oblique angle using consumer-grade smart cameras. Two such cameras were mounted at an elevation of 10 m and acquired images for a period of two months during the growing period of 2014. The field experiment consisted of nine barley cultivars that were cultivated in multiple repetitions and nitrogen treatments. Manual plant height measurements were carried out at four dates during the observation period. The software packages Agisoft PhotoScan, VisualSfM with CMVS/PMVS2 and SURE are investigated. The point clouds are georeferenced through a set of ground control points. Where adequate results are reached, a statistical analysis is performed.

  10. Tuning 3D topography on biomimetic surface for efficient self-cleaning and microfluidic manipulation

    NASA Astrophysics Data System (ADS)

    Guan, Wei-Sheng; Huang, Han-Xiong; Chen, An-Fu

    2015-03-01

    Currently, micro-/nanotopography on polymeric replica is generally limited to 2D when a mechanical demolding approach is applied. In this work, one-step replication of bio-inspired 3D topography is achieved using microinjection compression molding with novel dual-layer molds. Using a proposed flexible template, the replica topography and wettability are highly tunable during molding. Moreover, dual-scale topography on the mold is developed by coating the micropatterned insert with submicron silica particles. Contact angle and roll-off angle measurements indicate the lotus leaf, rose petal and rice leaf effects on biomimetic surfaces. Among the three kinds of surfaces, the petal-inspired surface possesses the superior performance in self-cleaning submicron contaminants and mechanical robustness, which is highly correlated to the low roughness-induced adhesive superhydrophobicity and the absence of fragile submicron-/nanostructure, respectively. Furthermore, a multi-layer mold structure is proposed for fabricating the open microfluidic devices. The embedment of the hydrophilic and hydrophobic silica particles in the microstructured open channel and the hydrophobic silica particles in the background area during replication renders the wettability contrast sharp, realizing the self-driven flow of microfluid confined within the open microchannel.

  11. 3D imaging of cone photoreceptors over extended time periods using optical coherence tomography with adaptive optics

    NASA Astrophysics Data System (ADS)

    Kocaoglu, Omer P.; Lee, Sangyeol; Jonnal, Ravi S.; Wang, Qiang; Herde, Ashley E.; Besecker, Jason; Gao, Weihua; Miller, Donald T.

    2011-03-01

    Optical coherence tomography with adaptive optics (AO-OCT) is a highly sensitive, noninvasive method for 3D imaging of the microscopic retina. The purpose of this study is to advance AO-OCT technology by enabling repeated imaging of cone photoreceptors over extended periods of time (days). This sort of longitudinal imaging permits monitoring of 3D cone dynamics in both normal and diseased eyes, in particular the physiological processes of disc renewal and phagocytosis, which are disrupted by retinal diseases such as age related macular degeneration and retinitis pigmentosa. For this study, the existing AO-OCT system at Indiana underwent several major hardware and software improvements to optimize system performance for 4D cone imaging. First, ultrahigh speed imaging was realized using a Basler Sprint camera. Second, a light source with adjustable spectrum was realized by integration of an Integral laser (Femto Lasers, λc=800nm, ▵λ=160nm) and spectral filters in the source arm. For cone imaging, we used a bandpass filter with λc=809nm and ▵λ=81nm (2.6 μm nominal axial resolution in tissue, and 167 KHz A-line rate using 1,408 px), which reduced the impact of eye motion compared to previous AO-OCT implementations. Third, eye motion artifacts were further reduced by custom ImageJ plugins that registered (axially and laterally) the volume videos. In two subjects, cone photoreceptors were imaged and tracked over a ten day period and their reflectance and outer segment (OS) lengths measured. High-speed imaging and image registration/dewarping were found to reduce eye motion to a fraction of a cone width (1 μm root mean square). The pattern of reflections in the cones was found to change dramatically and occurred on a spatial scale well below the resolution of clinical instruments. Normalized reflectance of connecting cilia (CC) and OS posterior tip (PT) of an exemplary cone was 54+/-4, 47+/-4, 48+/-6, 50+/-5, 56+/-1% and 46+/-4, 53+/-4, 52+/-6, 50+/-5, 44

  12. Optical low-cost and portable arrangement for full field 3D displacement measurement using a single camera

    NASA Astrophysics Data System (ADS)

    López-Alba, E.; Felipe-Sesé, L.; Schmeer, S.; Díaz, F. A.

    2016-11-01

    In the current paper, an optical low-cost system for 3D displacement measurement based on a single camera and 3D digital image correlation is presented. The conventional 3D-DIC set-up based on a two-synchronized-cameras system is compared with a proposed pseudo-stereo portable system that employs a mirror system integrated in a device for a straightforward application achieving a novel handle and flexible device for its use in many scenarios. The proposed optical system splits the image by the camera into two stereo images of the object. In order to validate this new approach and quantify its uncertainty compared to traditional 3D-DIC systems, solid rigid in and out-of-plane displacements experiments have been performed and analyzed. The differences between both systems have been studied employing an image decomposition technique which performs a full image comparison. Therefore, results of all field of view are compared with those using a stereoscopy system and 3D-DIC, discussing the accurate results obtained with the proposed device not having influence any distortion or aberration produced by the mirrors. Finally, the adaptability of the proposed system and its accuracy has been tested performing quasi-static and dynamic experiments using a silicon specimen under high deformation. Results have been compared and validated with those obtained from a conventional stereoscopy system showing an excellent level of agreement.

  13. Reproducibility of 3D kinematics and surface electromyography measurements of mastication.

    PubMed

    Remijn, Lianne; Groen, Brenda E; Speyer, Renée; van Limbeek, Jacques; Nijhuis-van der Sanden, Maria W G

    2016-03-01

    The aim of this study was to determine the measurement reproducibility for a procedure evaluating the mastication process and to estimate the smallest detectable differences of 3D kinematic and surface electromyography (sEMG) variables. Kinematics of mandible movements and sEMG activity of the masticatory muscles were obtained over two sessions with four conditions: two food textures (biscuit and bread) of two sizes (small and large). Twelve healthy adults (mean age 29.1 years) completed the study. The second to the fifth chewing cycle of 5 bites were used for analyses. The reproducibility per outcome variable was calculated with an intraclass correlation coefficient (ICC) and a Bland-Altman analysis was applied to determine the standard error of measurement relative error of measurement and smallest detectable differences of all variables. ICCs ranged from 0.71 to 0.98 for all outcome variables. The outcome variables consisted of four bite and fourteen chewing cycle variables. The relative standard error of measurement of the bite variables was up to 17.3% for 'time-to-swallow', 'time-to-transport' and 'number of chewing cycles', but ranged from 31.5% to 57.0% for 'change of chewing side'. The relative standard error of measurement ranged from 4.1% to 24.7% for chewing cycle variables and was smaller for kinematic variables than sEMG variables. In general, measurements obtained with 3D kinematics and sEMG are reproducible techniques to assess the mastication process. The duration of the chewing cycle and frequency of chewing were the best reproducible measurements. Change of chewing side could not be reproduced. The published measurement error and smallest detectable differences will aid the interpretation of the results of future clinical studies using the same study variables.

  14. Analytic 3D Imaging of Mammalian Nucleus at Nanoscale Using Coherent X-Rays and Optical Fluorescence Microscopy

    PubMed Central

    Song, Changyong; Takagi, Masatoshi; Park, Jaehyun; Xu, Rui; Gallagher-Jones, Marcus; Imamoto, Naoko; Ishikawa, Tetsuya

    2014-01-01

    Despite the notable progress that has been made with nano-bio imaging probes, quantitative nanoscale imaging of multistructured specimens such as mammalian cells remains challenging due to their inherent structural complexity. Here, we successfully performed three-dimensional (3D) imaging of mammalian nuclei by combining coherent x-ray diffraction microscopy, explicitly visualizing nuclear substructures at several tens of nanometer resolution, and optical fluorescence microscopy, cross confirming the substructures with immunostaining. This demonstrates the successful application of coherent x-rays to obtain the 3D ultrastructure of mammalian nuclei and establishes a solid route to nanoscale imaging of complex specimens. PMID:25185543

  15. A new optimization approach for the calibration of an ultrasound probe using a 3D optical localizer.

    PubMed

    Dardenne, G; Cano, J D Gil; Hamitouche, C; Stindel, E; Roux, C

    2007-01-01

    This paper describes a fast procedure for the calibration of an ultrasound (US) probe using a 3D optical localizer. This calibration step allows us to obtain the 3D position of any point located on the 2D ultrasonic (US) image. To carry out correctly this procedure, a phantom of known geometric properties is probed and these geometries are found in the US images. A segmentation step is applied in order to obtain automatically the needed information in the US images and then, an optimization approach is performed to find the optimal calibration parameters. A new optimization method to estimate the calibration parameters for an ultrasound (US) probe is developed.

  16. 3-D Surface Visualization of pH Titration "Topos": Equivalence Point Cliffs, Dilution Ramps, and Buffer Plateaus

    ERIC Educational Resources Information Center

    Smith, Garon C.; Hossain, Md Mainul; MacCarthy, Patrick

    2014-01-01

    3-D topographic surfaces ("topos") can be generated to visualize how pH behaves during titration and dilution procedures. The surfaces are constructed by plotting computed pH values above a composition grid with volume of base added in one direction and overall system dilution on the other. What emerge are surface features that…

  17. Skyrmion-induced bound states on the surface of 3D Topological Insulators

    NASA Astrophysics Data System (ADS)

    Andrikopoulos, Dimitrios; Soree, Bart

    In this work, we study the interaction between the surface state of a 3D Topological Insulator and a skyrmion magnetic texture. The skyrmion texture couples to the spin of the surface state electron with strength ΔS. Vortex and hedgehog skyrmion and anti-skyrmion structures are considered and their interaction is compared. Due to the vortex structure, the interaction of the in-plane components can be neglected and a step function is used to describe the skyrmion magnetization profile. In the hedgehog case, it is shown that the in-plane components cannot be disregarded and thus a realistic description for the skyrmion is required. Working in the micromagnetic framework, we derive a macrospin description for the skyrmion using the variational principle and then numerically solve for the bound states. It is shown that the existense and properties of these states as a function of skyrmion size, strongly depend on the skyrmion type. Both vortex and hedgehog skyrmions or anti-skyrmions can induce bound states with energies | E | < ΔS . For the hedgehog skyrmion case however, bound state appearance depends on the chirality. Finally, the probability densities in these states are computed and it is demonstrated that the electrons are localized throughout the skyrmion region. Also affiliated with imec, Belgium.

  18. Surface modeling and segmentation of the 3D airway wall in MSCT

    NASA Astrophysics Data System (ADS)

    Ortner, Margarete; Fetita, Catalin; Brillet, Pierre-Yves; Pr"teux, Françoise; Grenier, Philippe

    2011-03-01

    Airway wall remodeling in asthma and chronic obstructive pulmonary disease (COPD) is a well-known indicator of the pathology. In this context, current clinical studies aim for establishing the relationship between the airway morphological structure and its function. Multislice computed tomography (MSCT) allows morphometric assessment of airways, but requires dedicated segmentation tools for clinical exploitation. While most of the existing tools are limited to cross-section measurements, this paper develops a fully 3D approach for airway wall segmentation. Such approach relies on a deformable model which is built up as a patient-specific surface model at the level of the airway lumen and deformed to reach the outer surface of the airway wall. The deformation dynamics obey a force equilibrium in a Lagrangian framework constrained by a vector field which avoids model self-intersections. The segmentation result allows a dense quantitative investigation of the airway wall thickness with a deeper insight at bronchus subdivisions than classic cross-section methods. The developed approach has been assessed both by visual inspection of 2D cross-sections, performed by two experienced radiologists on clinical data obtained with various protocols, and by using a simulated ground truth (pulmonary CT image model). The results confirmed a robust segmentation in intra-pulmonary regions with an error in the range of the MSCT image resolution and underlined the interest of the volumetric approach versus purely 2D methods.

  19. Correlations of Surface Deformation and 3D Flow Field in a Compliant Wall Turbulent Channel Flow.

    NASA Astrophysics Data System (ADS)

    Wang, Jin; Zhang, Cao; Katz, Joseph

    2015-11-01

    This study focuses on the correlations between surface deformation and flow features, including velocity, vorticity and pressure, in a turbulent channel flow over a flat, compliant Polydimethylsiloxane (PDMS) wall. The channel centerline velocity is 2.5 m/s, and the friction Reynolds number is 2.3x103. Analysis is based on simultaneous measurements of the time resolved 3D velocity and surface deformation using tomographic PIV and Mach-Zehnder Interferometry. The volumetric pressure distribution is calculated plane by plane by spatially integrating the material acceleration using virtual boundary, omni-directional method. Conditional sampling based on local high/low pressure and deformation events reveals the primary flow structures causing the deformation. High pressure peaks appear at the interface between sweep and ejection, whereas the negative deformations peaks (dent) appear upstream, under the sweeps. The persistent phase lag between flow and deformations are presumably caused by internal damping within the PDMS. Some of the low pressure peaks and strong ejections are located under the head of hairpin vortices, and accordingly, are associated with positive deformation (bump). Others bumps and dents are correlated with some spanwise offset large inclined quasi-streamwise vortices that are not necessarily associated with hairpins. Sponsored by ONR.

  20. Euro-Maps 3D- A Transnational, High-Resolution Digital Surface Model For Europe

    NASA Astrophysics Data System (ADS)

    Uttenthaler, A.; Barner, F.; Hass, T.; Makiola, J.; d'Angelo, P.; Reinartz, P.; Carl, S.; Steiner, K.

    2013-12-01

    Euro-Maps 3D is a homogeneous 5 m spaced digital surface model (DSM) semi-automatically derived by Euromap from 2.5 m in-flight stereo data provided by the Indian IRS-P5 Cartosat-1 satellite. This new and innovative product has been developed in close co- operation with the Remote Sensing Technology Institute (IMF) of the German Aerospace Center (DLR) and is being jointly exploited. The very detailed and accurate representation of the surface is achieved by using a sophisticated and well adapted algorithm implemented on the basis of the Semi-Global Matching approach. In addition, the final product includes detailed flanking information consisting of several pixel-based quality and traceability layers also including an ortho layer. The product is believed to provide maximum accuracy and transparency. The DSM product meets and exceeds HRE80 qualification standards. The DSM product will be made available transnational in a homogeneous quality for most parts of Europe, North Africa and Turkey by Euromap step-by-step. Other areas around the world are processed on demand.

  1. Robust ego-motion estimation and 3-D model refinement using surface parallax.

    PubMed

    Agrawal, Amit; Chellappa, Rama

    2006-05-01

    We present an iterative algorithm for robustly estimating the ego-motion and refining and updating a coarse depth map using parametric surface parallax models and brightness derivatives extracted from an image pair. Given a coarse depth map acquired by a range-finder or extracted from a digital elevation map (DEM), ego-motion is estimated by combining a global ego-motion constraint and a local brightness constancy constraint. Using the estimated camera motion and the available depth estimate, motion of the three-dimensional (3-D) points is compensated. We utilize the fact that the resulting surface parallax field is an epipolar field, and knowing its direction from the previous motion estimates, estimate its magnitude and use it to refine the depth map estimate. The parallax magnitude is estimated using a constant parallax model (CPM) which assumes a smooth parallax field and a depth based parallax model (DBPM), which models the parallax magnitude using the given depth map. We obtain confidence measures for determining the accuracy of the estimated depth values which are used to remove regions with potentially incorrect depth estimates for robustly estimating ego-motion in subsequent iterations. Experimental results using both synthetic and real data (both indoor and outdoor sequences) illustrate the effectiveness of the proposed algorithm.

  2. 3D Dynamics of Freshwater Lenses in the Near-Surface Layer of the Tropical Ocean

    NASA Astrophysics Data System (ADS)

    Soloviev, Alexander; Dean, Cayla

    2015-04-01

    Convective rains in the Intertropical Convergence Zone (ITCZ) produce lenses of freshened water on the ocean surface. These lenses are localized in space and typically involve both salinity and temperature anomalies. Due to significant density anomalies, strong pressure gradients develop, which result in lateral spreading of freshwater lenses in a form resembling gravity currents. Gravity currents inherently involve three-dimensional dynamics. As a type of organized structure, gravity currents in the upper layer of the ocean may also interact with, and be shaped by, the ambient oceanic environment and atmospheric conditions. Among the important factors are the background stratification, wind stress, wind/wave mixing and spatially coherent organized motions in the near-surface layer of the ocean. Under certain conditions, a resonant interaction between a propagating freshwater lens and internal waves in the underlying pycnocline (e.g., barrier layer) may develop, whereas interaction with wind stress may produce an asymmetry in the freshwater lens and associated mixing. These two types of interactions working in concert may explain the series of sharp frontal interfaces, which have been observed in association with freshwater lenses during TOGA COARE. In this work, we have conducted a series of numerical experiments using computational fluid dynamics tools. These numerical simulations were designed to elucidate the relationship between vertical mixing and horizontal advection of salinity under various environmental conditions and potential impact on the Aquarius and SMOS satellite image formation. Available near-surface data from field experiments served as a guidance for numerical simulations. The results of this study indicate that 3D dynamics of freshwater lenses are essential within a certain range of wind/wave conditions and the freshwater influx in the surface layer of the ocean.

  3. FreeCAD visualization of realistic 3D physical optics beams within a CAD system-model

    NASA Astrophysics Data System (ADS)

    Gayer, D.; O'Sullivan, C.; Scully, S.; Burke, D.; Brossard, J.; Chapron, C.

    2016-07-01

    The facility to realise the shape and extent of optical beams within a telescope or beamcombiner can aid greatly in the design and layout of optical elements within the system. It can also greatly facilitate communication between the optical design team and other teams working on the mechanical design of an instrument. Beyond the realm where raytracing is applicable however, it becomes much more difficult to realise accurate 3D beams which incorporate diffraction effects. It then is another issue to incorporate this into a CAD model of the system. A novel method is proposed which has been used to aid with the design of an optical beam combiner for the QUBIC (Q and U Bolometric Interferometer for Cosmology) 1 experiment operating at 150 GHz and 220 GHz. The method combines calculation work in GRASP 2, a commercial physical optics modelling tool from TICRA, geometrical work in Mathematica, and post processing in MATLAB. Finally, the Python console of the open source package FreeCAD3 is exploited to realise the 3D beams in a complete CAD system-model of the QUBIC optical beam combiner. This paper details and explains the work carried out to reach the goal and presents some graphics of the outcome. 3D representations of beams from some back-to-back input horns of the QUBIC instrument are shown within the CAD model. Beams of the -3dB and -13dB contour envelope are shown as well as envelopes enclosing 80% and 95% of the power of the beam. The ability to see these beams in situ with all the other elements of the combiner such as mirrors, cold stop, beam splitter and cryostat widows etc. greatly simplified the design for these elements and facilitated communication of element dimension and location between different subgroups within the QUBIC group.

  4. Automated in vivo 3D high-definition optical coherence tomography skin analysis system.

    PubMed

    Ai Ping Yow; Jun Cheng; Annan Li; Srivastava, Ruchir; Jiang Liu; Wong, Damon Wing Kee; Hong Liang Tey

    2016-08-01

    The in vivo assessment and visualization of skin structures can be performed through the use of high resolution optical coherence tomography imaging, also known as HD-OCT. However, the manual assessment of such images can be exhaustive and time consuming. In this paper, we present an analysis system to automatically identify and quantify the skin characteristics such as the topography of the surface of the skin and thickness of the epidermis in HD-OCT images. Comparison of this system with manual clinical measurements demonstrated its potential for automatic objective skin analysis and diseases diagnosis. To our knowledge, this is the first report of an automated system to process and analyse HD-OCT skin images.

  5. Q AS A LITHOLOGICAL/HYDROCARBON INDICATOR: FROM FULL WAVEFORM SONIC TO 3D SURFACE SEISMIC

    SciTech Connect

    Jorge O. Parra; C.L. Hackert; L. Wilson; H.A. Collier; J. Todd Thomas

    2006-03-31

    The goal of this project was to develop a method to exploit viscoelastic rock and fluid properties to greatly enhance the sensitivity of surface seismic measurements to the presence of hydrocarbon saturation. To reach the objective, Southwest Research Institute scientists used well log, lithology, production, and 3D seismic data from an oil reservoir located on the Waggoner Ranch in north central Texas. The project was organized in three phases. In the first phase, we applied modeling techniques to investigate seismic- and acoustic-frequency wave attenuation and its effect on observable wave attributes. We also gathered existing data and acquired new data from the Waggoner Ranch field, so that all needed information was in place for the second phase. During the second phase, we developed methods to extract attenuation from borehole acoustic and surface seismic data. These methods were tested on synthetic data constructed from realistic models and real data. In the third and final phase of the project, we applied this technology to a full data set from the Waggoner site. The results presented in this Final Report show that geological conditions at the site did not allow us to obtain interpretable results from the Q processing algorithm for 3D seismic data. However, the Q-log processing algorithm was successfully applied to full waveform sonic data from the Waggoner site. A significant part of this project was technology transfer. We have published several papers and conducted presentations at professional conferences. In particular, we presented the Q-log algorithm and applications at the Society of Exploration Geophysicists (SEG) Development and Production Forum in Austin, Texas, in May 2005. The presentation attracted significant interest from the attendees and, at the request of the SEG delegates, it was placed on the Southwest Research Institute Internet site. The presentation can be obtained from the following link: http://www.swri.org/4org/d15/elecsys

  6. Intraoperative handheld probe for 3D imaging of pediatric benign vocal fold lesions using optical coherence tomography (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Benboujja, Fouzi; Garcia, Jordan; Beaudette, Kathy; Strupler, Mathias; Hartnick, Christopher J.; Boudoux, Caroline

    2016-02-01

    Excessive and repetitive force applied on vocal fold tissue can induce benign vocal fold lesions. Children affected suffer from chronic hoarseness. In this instance, the vibratory ability of the folds, a complex layered microanatomy, becomes impaired. Histological findings have shown that lesions produce a remodeling of sup-epithelial vocal fold layers. However, our understanding of lesion features and development is still limited. Indeed, conventional imaging techniques do not allow a non-invasive assessment of sub-epithelial integrity of the vocal fold. Furthermore, it remains challenging to differentiate these sub-epithelial lesions (such as bilateral nodules, polyps and cysts) from a clinical perspective, as their outer surfaces are relatively similar. As treatment strategy differs for each lesion type, it is critical to efficiently differentiate sub-epithelial alterations involved in benign lesions. In this study, we developed an optical coherence tomography (OCT) based handheld probe suitable for pediatric laryngological imaging. The probe allows for rapid three-dimensional imaging of vocal fold lesions. The system is adapted to allow for high-resolution intra-operative imaging. We imaged 20 patients undergoing direct laryngoscopy during which we looked at different benign pediatric pathologies such as bilateral nodules, cysts and laryngeal papillomatosis and compared them to healthy tissue. We qualitatively and quantitatively characterized laryngeal pathologies and demonstrated the added advantage of using 3D OCT imaging for lesion discrimination and margin assessment. OCT evaluation of the integrity of the vocal cord could yield to a better pediatric management of laryngeal diseases.

  7. A compact acousto-optic lens for 2D and 3D femtosecond based 2-photon microscopy

    PubMed Central

    Kirkby, Paul A.; Naga Srinivas, N.K.M.; Silver, R. Angus

    2010-01-01

    We describe a high speed 3D Acousto-Optic Lens Microscope (AOLM) for femtosecond 2-photon imaging. By optimizing the design of the 4 AO Deflectors (AODs) and by deriving new control algorithms, we have developed a compact spherical AOL with a low temporal dispersion that enables 2-photon imaging at 10-fold lower power than previously reported. We show that the AOLM can perform high speed 2D raster-scan imaging (>150 Hz) without scan rate dependent astigmatism. It can deflect and focus a laser beam in a 3D random access sequence at 30 kHz and has an extended focusing range (>137 μm; 40X 0.8NA objective). These features are likely to make the AOLM a useful tool for studying fast physiological processes distributed in 3D space PMID:20588506

  8. [3-D endocardial surface modelling based on the convex hull algorithm].

    PubMed

    Lu, Ying; Xi, Ri-hui; Shen, Hai-dong; Ye, You-li; Zhang, Yong

    2006-11-01

    In this paper, a method based on the convex hull algorithm is presented for extracting modelling data from the locations of catheter electrodes within a cardiac chamber, so as to create a 3-D model of the heart chamber during diastole and to obtain a good result in the 3-D reconstruction of the chamber based on VTK.

  9. 3D measurement for rapid prototyping

    NASA Astrophysics Data System (ADS)

    Albrecht, Peter; Lilienblum, Tilo; Sommerkorn, Gerd; Michaelis, Bernd

    1996-08-01

    Optical 3-D measurement is an interesting approach for rapid prototyping. On one hand it's necessary to get the 3-D data of an object and on the other hand it's necessary to check the manufactured object (quality checking). Optical 3-D measurement can realize both. Classical 3-D measurement procedures based on photogrammetry cause systematic errors at strongly curved surfaces or steps in surfaces. One possibility to reduce these errors is to calculate the 3-D coordinates from several successively taken images. Thus it's possible to get higher spatial resolution and to reduce the systematic errors at 'problem surfaces.' Another possibility is to process the measurement values by neural networks. A modified associative memory smoothes and corrects the calculated 3-D coordinates using a-priori knowledge about the measurement object.

  10. Combination of 3D skin surface texture features and 2D ABCD features for improved melanoma diagnosis.

    PubMed

    Ding, Yi; John, Nigel W; Smith, Lyndon; Sun, Jiuai; Smith, Melvyn

    2015-10-01

    Two-dimensional asymmetry, border irregularity, colour variegation and diameter (ABCD) features are important indicators currently used for computer-assisted diagnosis of malignant melanoma (MM); however, they often prove to be insufficient to make a convincing diagnosis. Previous work has demonstrated that 3D skin surface normal features in the form of tilt and slant pattern disruptions are promising new features independent from the existing 2D ABCD features. This work investigates that whether improved lesion classification can be achieved by combining the 3D features with the 2D ABCD features. Experiments using a nonlinear support vector machine classifier show that many combinations of the 2D ABCD features and the 3D features can give substantially better classification accuracy than using (1) single features and (2) many combinations of the 2D ABCD features. The best 2D and 3D feature combination includes the overall 3D skin surface disruption, the asymmetry and all the three colour channel features. It gives an overall 87.8 % successful classification, which is better than the best single feature with 78.0 % and the best 2D feature combination with 83.1 %. These demonstrate that (1) the 3D features have additive values to improve the existing lesion classification and (2) combining the 3D feature with all the 2D features does not lead to the best lesion classification. The two ABCD features not selected by the best 2D and 3D combination, namely (1) the border feature and (2) the diameter feature, were also studied in separate experiments. It found that inclusion of either feature in the 2D and 3D combination can successfully classify 3 out of 4 lesion groups. The only one group not accurately classified by either feature can be classified satisfactorily by the other. In both cases, they have shown better classi