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Sample records for 4 5 7

  1. 9. (5 X 7 enlargement from 4 X 5 negative) ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. (5 X 7 enlargement from 4 X 5 negative) FIRST FLOOR, WINDOW MOLDING ON SOUTH WALL LOOKING SOUTH - Sites Homestead, Monongahela National Forest (Tract 390) East of Route 28, Seneca Rocks, Pendleton County, WV

  2. Praxis release notes, Versions 7. 4 and 7. 5

    SciTech Connect

    Holloway, F.W.

    1985-09-02

    These release notes are intended as a guide to those responsible for Nova software. They assume extensive knowledge of the present Praxis language. Each improvement made in Praxis versions 7.4 and 7.5 is described. The descriptions are organized as shown in the table of contents. Many of the improvements have example programs which are contained within the chapter on example programs. For completeness, the final chapter lists the specific areas within the compiler which were modified. These will only be useful to those working on the compiler itself.

  3. 7 CFR 773.4-773.5 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 7 2013-01-01 2013-01-01 false 773.4-773.5 Section 773.4-773.5 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS SPECIAL APPLE LOAN PROGRAM §§ 773.4-773.5...

  4. 7 CFR 773.4-773.5 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 7 2011-01-01 2011-01-01 false 773.4-773.5 Section 773.4-773.5 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS SPECIAL APPLE LOAN PROGRAM §§ 773.4-773.5...

  5. 7 CFR 773.4-773.5 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 7 2014-01-01 2014-01-01 false 773.4-773.5 Section 773.4-773.5 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS SPECIAL APPLE LOAN PROGRAM §§ 773.4-773.5...

  6. 7 CFR 773.4-773.5 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false 773.4-773.5 Section 773.4-773.5 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS SPECIAL APPLE LOAN PROGRAM §§ 773.4-773.5...

  7. 7 CFR 773.4-773.5 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 7 2012-01-01 2012-01-01 false 773.4-773.5 Section 773.4-773.5 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS SPECIAL APPLE LOAN PROGRAM §§ 773.4-773.5...

  8. Differential Activation of Vascular Smooth Muscle Kv7.4, Kv7.5, and Kv7.4/7.5 Channels by ML213 and ICA-069673

    PubMed Central

    Brueggemann, Lyubov I.; Haick, Jennifer M.; Cribbs, Leanne L.

    2014-01-01

    Recent research suggests that smooth muscle cells express Kv7.4 and Kv7.5 voltage-activated potassium channels, which contribute to maintenance of their resting membrane voltage. New pharmacologic activators of Kv7 channels, ML213 (N-mesitybicyclo[2.2.1]heptane-2-carboxamide) and ICA-069673 N-(6-chloropyridin-3-yl)-3,4-difluorobenzamide), have been reported to discriminate among channels formed from different Kv7 subtypes. We compared the effects of ML213 and ICA-069673 on homomeric human Kv7.4, Kv7.5, and heteromeric Kv7.4/7.5 channels exogenously expressed in A7r5 vascular smooth muscle cells. We found that, despite its previous description as a selective activator of Kv7.2 and Kv7.4, ML213 significantly increased the maximum conductance of homomeric Kv7.4 and Kv7.5, as well as heteromeric Kv7.4/7.5 channels, and induced a negative shift of their activation curves. Current deactivation rates decreased in the presence of the ML213 (10 μM) for all three channel combinations. Mutants of Kv7.4 (W242L) and Kv7.5 (W235L), previously found to be insensitive to another Kv7 channel activator, retigabine, were also insensitive to ML213 (10 μM). In contrast to ML213, ICA-069673 robustly activated Kv7.4 channels but was significantly less effective on homomeric Kv7.5 channels. Heteromeric Kv7.4/7.5 channels displayed intermediate responses to ICA-069673. In each case, ICA-069673 induced a negative shift of the activation curves without significantly increasing maximal conductance. Current deactivation rates decreased in the presence of ICA-069673 in a subunit-specific manner. Kv7.4 W242L responded to ICA-069673-like wild-type Kv7.4, but a Kv7.4 F143A mutant was much less sensitive to ICA-069673. Based on these results, ML213 and ICA-069673 likely bind to different sites and are differentially selective among Kv7.4, Kv7.5, and Kv7.4/7.5 channel subtypes. PMID:24944189

  9. Crystal structure of 2-amino-7,7-dimethyl-5-oxo-4-(pyridin-4-yl)-5,6,7,8-tetrahydro-4 H-chromene-3-carbonitrile hemihydrate

    NASA Astrophysics Data System (ADS)

    Sharma, N.; Banerjee, B.; Brahmachari, G.; Kant, R.; Gupta, V. K.

    2015-12-01

    The title compound, 2-amino-7,7-dimethyl-5-oxo-4-(pyridin-4-yl)-5,6,7,8-tetrahydro-4 Hchromene-3-carbonitrile was synthesized by multicomponent reaction at room temperature using commercially available urea as inexpensive and environmentally benign organo-catalyst. Its structure was determined by X-ray structure analysis. The crystals are monoclinic, sp. gr. C2/ c, a = 22.010(6), b = 11.0364(10), c = 17.147(4) Å, β = 130.37(4)°, Z = 8, R = 0.0433 for 2377 observed reflections.

  10. 40 CFR 721.9504 - Silane, triethoxy (3,3,4,4,5,5, 6,6,7,7,8,8,8-tridecafluorooctyl)-.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Silane, triethoxy (3,3,4,4,5,5, 6,6,7... Significant New Uses for Specific Chemical Substances § 721.9504 Silane, triethoxy (3,3,4,4,5,5, 6,6,7,7,8,8,8... substance identified as silane, triethoxy (3,3,4,4,5, 5,6,6,7,7,8,8,8-tridecafluorooctyl)- (PMN...

  11. 40 CFR 721.9504 - Silane, triethoxy (3,3,4,4,5,5, 6,6,7,7,8,8,8-tridecafluorooctyl)-.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Silane, triethoxy (3,3,4,4,5,5, 6,6,7... Significant New Uses for Specific Chemical Substances § 721.9504 Silane, triethoxy (3,3,4,4,5,5, 6,6,7,7,8,8,8... substance identified as silane, triethoxy (3,3,4,4,5, 5,6,6,7,7,8,8,8-tridecafluorooctyl)- (PMN...

  12. 40 CFR 721.9504 - Silane, triethoxy (3,3,4,4,5,5, 6,6,7,7,8,8,8-tridecafluorooctyl)-.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Silane, triethoxy (3,3,4,4,5,5, 6,6,7... Significant New Uses for Specific Chemical Substances § 721.9504 Silane, triethoxy (3,3,4,4,5,5, 6,6,7,7,8,8,8... substance identified as silane, triethoxy (3,3,4,4,5, 5,6,6,7,7,8,8,8-tridecafluorooctyl)- (PMN...

  13. 40 CFR 721.9504 - Silane, triethoxy (3,3,4,4,5,5, 6,6,7,7,8,8,8-tridecafluorooctyl)-.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Silane, triethoxy (3,3,4,4,5,5, 6,6,7... Significant New Uses for Specific Chemical Substances § 721.9504 Silane, triethoxy (3,3,4,4,5,5, 6,6,7,7,8,8,8... substance identified as silane, triethoxy (3,3,4,4,5, 5,6,6,7,7,8,8,8-tridecafluorooctyl)- (PMN...

  14. 40 CFR 721.9504 - Silane, triethoxy (3,3,4,4,5,5, 6,6,7,7,8,8,8-tridecafluorooctyl)-.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Silane, triethoxy (3,3,4,4,5,5, 6,6,7... Significant New Uses for Specific Chemical Substances § 721.9504 Silane, triethoxy (3,3,4,4,5,5, 6,6,7,7,8,8,8... substance identified as silane, triethoxy (3,3,4,4,5, 5,6,6,7,7,8,8,8-tridecafluorooctyl)- (PMN...

  15. 7. Photographic copy of photograph (from original 4 x 5 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Photographic copy of photograph (from original 4 x 5 black and white print in the Army Port Contractors' 'Completion Report' at the Engineering Office, Oakland Army Base, California). Photograph taken January 28, 1942 by unknown photographer. AT CENTER RIGHT, SOUTH AND EAST SIDES OBLIQUE VIEW OF POST HEADQUARTERS BUILDING (ADMINISTRATION BUILDING, BLDG. 1) TAKEN FROM EAST SIDE OF MARITIME STREET. - Oakland Army Base, Maritime Street at West Grand Avenue, Oakland, Alameda County, CA

  16. 7. Photographic copy (reduced to 4 x 5 from 8 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Photographic copy (reduced to 4 x 5 from 8 X 10 black and white paper reproduction in 1941 appraisal by E.E. Malloy at the Engineering Office, Oakland Army Base, California). Photograph taken between June 1940 and January 1941 by unknown photographer. PARTIAL SOUTH ELEVATION OF VEHICLE MAINTENANCE SHOP (BLDG. 99). - Oakland Army Base, Vehicle Maintenance Shop, Attu Street & Corregidor Avenue, Oakland, Alameda County, CA

  17. 40 CFR 721.9503 - Silane, (3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluorodecyl)trimethoxy-.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Silane, (3,3,4,4,5,5,6,6,7,7,8,8,9,9... SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.9503 Silane, (3,3,4,4,5,5,6,6,7,7,8,8... subject to reporting. (1) The chemical substance identified as silane,...

  18. 40 CFR 721.9503 - Silane, (3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluorodecyl)trimethoxy-.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Silane, (3,3,4,4,5,5,6,6,7,7,8,8,9,9... SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.9503 Silane, (3,3,4,4,5,5,6,6,7,7,8,8... subject to reporting. (1) The chemical substance identified as silane,...

  19. 40 CFR 721.9503 - Silane, (3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluorodecyl)trimethoxy-.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Silane, (3,3,4,4,5,5,6,6,7,7,8,8,9,9... SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.9503 Silane, (3,3,4,4,5,5,6,6,7,7,8,8... subject to reporting. (1) The chemical substance identified as silane,...

  20. 40 CFR 721.9503 - Silane, (3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluorodecyl)trimethoxy-.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Silane, (3,3,4,4,5,5,6,6,7,7,8,8,9,9... SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.9503 Silane, (3,3,4,4,5,5,6,6,7,7,8,8... subject to reporting. (1) The chemical substance identified as silane,...

  1. 40 CFR 721.9503 - Silane, (3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluorodecyl)trimethoxy-.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Silane, (3,3,4,4,5,5,6,6,7,7,8,8,9,9... SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.9503 Silane, (3,3,4,4,5,5,6,6,7,7,8,8... subject to reporting. (1) The chemical substance identified as silane,...

  2. Two potent suicide substrates of mushroom tyrosinase: 7,8,4'-trihydroxyisoflavone and 5,7,8,4'-tetrahydroxyisoflavone.

    PubMed

    Chang, Te-Sheng

    2007-03-01

    The inhibitory characteristics of two isoflavone metabolites, 7,8,4'-trihydroxyisoflavone and 5,7,8,4'-tetrahydroxyisoflavone, on mushroom tyrosinase were investigated. The two isoflavones were isolated from soygerm koji and inhibited both monophenolase and diphenolase activities of tyrosinase. Their inhibition type was demonstrated to be irreversible inhibition by preincubation and recovery experiments. By using HPLC analysis, it was found that mushroom tyrosinase could catalyze the two isoflavones. These results revealed that the two isoflavones belonged to suicide substrates of mushroom tyrosinase. The partition ratios between molecules of suicide substrate in the formation of product and in the inactivation of enzyme were determined to be 81.7 +/- 5.9 and 35.5 +/- 3.8 for 7,8,4'-trihydroxyisoflavone and 5,7,8,4'-tetrahydroxyisoflavone, respectively. From kinetic studies, maximal inactivation rate constants and Michaelis constants were 0.79 +/- 0.08 and 1.01 +/- 0.04 min(-1) and 18.7 +/- 2.31 and 7.81 +/- 0.05 microM for 7,8,4'-trihydroxyisoflavone and 5,7,8,4'-tetrahydroxyisoflavone, respectively, when L-DOPA was used as the enzyme substrate. Structure analysis comparing the inactivating activity between the two isoflavones and their structure analogues showed that not only the 7,8-dihydroxyl groups but also the isoflavone skeleton of the two isoflavones played an important role in inactivating tyrosinase activity. The present study demonstrated that 7,8,4'-trihydroxyisoflavone and 5,7,8,4'-tetrahydroxyisoflavone are potent suicide substrates of mushroom tyrosinase. PMID:17295516

  3. 40 CFR 721.5280 - 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with diazotized 4-butylbenzenamine...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...-amino-5-hydroxy-, coupled with diazotized 4-butylbenzenamine, diazotized 4,4â²-cyclohexylidenebis and m... Specific Chemical Substances § 721.5280 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with... 2,7-naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with diazotized...

  4. 40 CFR 721.5280 - 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with diazotized 4-butylbenzenamine...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...-amino-5-hydroxy-, coupled with diazotized 4-butylbenzenamine, diazotized 4,4â²-cyclohexylidenebis and m... Specific Chemical Substances § 721.5280 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with... 2,7-naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with diazotized...

  5. 40 CFR 721.5280 - 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with diazotized 4-butylbenzenamine...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Specific Chemical Substances § 721.5280 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with... 2,7-naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with diazotized 4-butylbenzenamine... 40 Protection of Environment 31 2014-07-01 2014-07-01 false 2,7-Naphthalenedisulfonic acid,...

  6. 40 CFR 721.5280 - 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with diazotized 4-butylbenzenamine...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Specific Chemical Substances § 721.5280 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with... 2,7-naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with diazotized 4-butylbenzenamine... 40 Protection of Environment 32 2012-07-01 2012-07-01 false 2,7-Naphthalenedisulfonic acid,...

  7. 40 CFR 721.5280 - 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with diazotized 4-butylbenzenamine...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Specific Chemical Substances § 721.5280 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with... 2,7-naphthalenedisulfonic acid, 4-amino-5-hydroxy-, coupled with diazotized 4-butylbenzenamine... 40 Protection of Environment 32 2013-07-01 2013-07-01 false 2,7-Naphthalenedisulfonic acid,...

  8. 5,7-Dihydroxy-6,4'-dimeth-oxyflavone.

    PubMed

    Mou, Ming-Yue; Pi, Ke; Zhang, Qi-Long; Zhang, Yun-Qian; Zhang, Qian-Jun

    2007-01-01

    In the title compound, C(17)H(14)O(6), the benzopyran ring system is essentially planar and forms a dihedral angle of 6.84 (4)° with the other benzene ring. In the crystal structure, centrosymmetrically related mol-ecules are linked into dimers by O-H⋯O hydrogen bonds. The crystal packing is controlled by C-H⋯π and π-π stacking inter-actions involving the benzopyran and benzene rings, with centroid-centroid distances between 3.645 (2) and 3.986 (2) Å. PMID:21200948

  9. 40 CFR 721.5262 - 2,7-Naphthalenedisulfonic acid, 5-[[4-chloro-6-[substituted] amino]-1,3,5-triazin-2-yl]amino]-4...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...,7-Naphthalenedisulfonic acid, 5- amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3- -, trisodium salt... identified generically as 2,7-Naphthalenedisulfonic acid, 5- amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3... 40 Protection of Environment 32 2012-07-01 2012-07-01 false 2,7-Naphthalenedisulfonic acid,...

  10. 40 CFR 721.5262 - 2,7-Naphthalenedisulfonic acid, 5-[[4-chloro-6-[substituted] amino]-1,3,5-triazin-2-yl]amino]-4...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...,7-Naphthalenedisulfonic acid, 5- amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3- -, trisodium salt... identified generically as 2,7-Naphthalenedisulfonic acid, 5- amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3... 40 Protection of Environment 32 2013-07-01 2013-07-01 false 2,7-Naphthalenedisulfonic acid,...

  11. 7 CFR 5.4 - Commodities for which parity prices shall be calculated.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 1 2012-01-01 2012-01-01 false Commodities for which parity prices shall be calculated. 5.4 Section 5.4 Agriculture Office of the Secretary of Agriculture DETERMINATION OF PARITY PRICES § 5.4 Commodities for which parity prices shall be calculated. Parity prices shall be calculated for the following commodities: Basic...

  12. 7 CFR 5.4 - Commodities for which parity prices shall be calculated.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 1 2010-01-01 2010-01-01 false Commodities for which parity prices shall be calculated. 5.4 Section 5.4 Agriculture Office of the Secretary of Agriculture DETERMINATION OF PARITY PRICES § 5.4 Commodities for which parity prices shall be calculated. Parity prices shall be calculated for the following commodities: Basic...

  13. Synthesis and biological activity of substituted-4,5,6,7-tetrahydrothieno pyridines: a review.

    PubMed

    Sangshetti, Jaiprakash N; Zambare, Abhay S; Khan, Firoz A Kalam; Gonjari, Indrajeet; Zaheer, Zahid

    2014-01-01

    4,5,6,7-Tetrahydrothieno pyridine is an important class of heterocyclic nucleus. Various 4,5,6,7-tetrahydrothieno pyridine derivatives have been synthesized and evaluated for various biological activities in different models with desired findings. Some analogs have shown potent biological activities and may be considered as lead molecule for the development of future drugs. Number of drug molecules are available in the market and many molecules are in clinical development containing 4,5,6,7-tetrahydrothieno pyridine nucleus as an important core. This review is an attempt to organize the chemical and biological aspects of 4,5,6,7-tetrahydrothieno pyridine analogs reported in last 20 year to till date. Review mainly focuses on the important role of the core in synthesis of drug or drug intermediates giving emphasis on synthetic schemes and biological activities of the different 4,5,6,7-tetrahydrothieno pyridine analogs. PMID:25373848

  14. Differential Protein Kinase C-dependent Modulation of Kv7.4 and Kv7.5 Subunits of Vascular Kv7 Channels*

    PubMed Central

    Brueggemann, Lioubov I.; Mackie, Alexander R.; Cribbs, Leanne L.; Freda, Jessica; Tripathi, Abhishek; Majetschak, Matthias; Byron, Kenneth L.

    2014-01-01

    The Kv7 family (Kv7.1–7.5) of voltage-activated potassium channels contributes to the maintenance of resting membrane potential in excitable cells. Previously, we provided pharmacological and electrophysiological evidence that Kv7.4 and Kv7.5 form predominantly heteromeric channels and that Kv7 activity is regulated by protein kinase C (PKC) in response to vasoconstrictors in vascular smooth muscle cells. Direct evidence for Kv7.4/7.5 heteromer formation, however, is lacking. Furthermore, it remains to be determined whether both subunits are regulated by PKC. Utilizing proximity ligation assays to visualize single molecule interactions, we now show that Kv7.4/Kv.7.5 heteromers are endogenously expressed in vascular smooth muscle cells. Introduction of dominant-negative Kv7.4 and Kv7.5 subunits in mesenteric artery myocytes reduced endogenous Kv7 currents by 84 and 76%, respectively. Expression of an inducible protein kinase Cα (PKCα) translocation system revealed that PKCα activation is sufficient to suppress endogenous Kv7 currents in A7r5 rat aortic and mesenteric artery smooth muscle cells. Arginine vasopressin (100 and 500 pm) and the PKC activator phorbol 12-myristate 13-acetate (1 nm) each inhibited human (h) Kv7.5 and hKv7.4/7.5, but not hKv7.4 channels expressed in A7r5 cells. A decrease in hKv7.5 and hKv7.4/7.5 current densities was associated with an increase in PKC-dependent phosphorylation of the channel proteins. These findings provide further evidence for a differential regulation of Kv7.4 and Kv7.5 channel subunits by PKC-dependent phosphorylation and new mechanistic insights into the role of heteromeric subunit assembly for regulation of vascular Kv7 channels. PMID:24297175

  15. 4-(3,7-Dimethyl-4-oxo-4,5-dihydro­isoxazolo[4,5-d]pyridazin-5-yl)benzene­sulfonamide

    PubMed Central

    Asiri, Abdullah M.; Faidallah, Hassan M.; Al-Youbi, Abdulrahman O.; Obaid, Abdullah.Y.; Ng, Seik Weng

    2011-01-01

    The nine-membered fused-ring system of the title pyridazine derivative, C13H12N4O4S, is approximately planar (r.m.s. deviation 0.027 Å), and the benzene ring of the phenyl­sulfamide substituent is aligned at 43.5 (1)° to the fused-ring system. The amine group of the sulfonamide substituent forms an N—H⋯O hydrogen bond to the ketonic O atom of two neigboring mol­ecules to generate a chain running along the c axis. PMID:22059027

  16. Enhanced OECD TG 407 in detection of endocrine-mediated effects of 4,4'-(octahydro-4,7-methano-5H-inden-5-ylidene)bisphenol.

    PubMed

    Umano, Takaaki; Miyata, Katsumi; Minobe, Yasushi; Yamasaki, Kanji

    2010-03-01

    The purpose of this study was to investigate whether the estrogenic effects were detected in the enhanced TG 407 if the estrogenic property was not so strong in the uterotrophic assay. The estrogenic property of 4,4'-(octahydro-4,7-methano-5H-inden-5-ylidene)bisphenol in the uterotrophic assay was slightly stronger than that of genistein or nonylphenol, but weaker than that of ethinyl estradiol. We performed a 28-day repeated-dose toxicity study (enhanced OECD test guideline No. 407) on 4,4'-(octahydro-4,7-methano-5H-inden-5-ylidene)bisphenol based on the OECD draft protocol. The test chemical, administered orally at doses of 0, 10, 50, and 250 mg/kg per day for at least 28 days, caused such estrogenic effects as abnormal estrous cycle, increased ovarian follicles, increased uterine epithelial height, and vaginal mucification in the 50 and/or 250 mg/kg groups. Moreover, follicular epithelial cell hyperplasia of the thyroid was detected in all male rats given the test chemical and in female rats in the 250 mg/kg group. It was concluded that the estrogenic effects were detected in growing rats given 4,4'-(octahydro-4,7-methano-5H-inden-5-ylidene)bisphenol, and thyroid dysfunction was also observed as the endocrine-mediated effects. PMID:19882337

  17. 40 CFR 721.5262 - 2,7-Naphthalenedisulfonic acid, 5-[[4-chloro-6-[substituted] amino]-1,3,5-triazin-2-yl]amino]-4...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...- amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3- -, trisodium salt (generic). 721.5262 Section 721.5262...,7-Naphthalenedisulfonic acid, 5- amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3- -, trisodium salt... identified generically as 2,7-Naphthalenedisulfonic acid, 5-......

  18. 40 CFR 721.5262 - 2,7-Naphthalenedisulfonic acid, 5-[[4-chloro-6-[substituted] amino]-1,3,5-triazin-2-yl]amino]-4...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...- amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3- -, trisodium salt (generic). 721.5262 Section 721.5262...,7-Naphthalenedisulfonic acid, 5- amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3- -, trisodium salt... identified generically as 2,7-Naphthalenedisulfonic acid, 5-......

  19. 8 CFR 1236.4 - Removal of S-5, S-6, and S-7 nonimmigrants.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Removal of S-5, S-6, and S-7 nonimmigrants... OF ALIENS ORDERED REMOVED Detention of Aliens Prior to Order of Removal § 1236.4 Removal of S-5, S-6, and S-7 nonimmigrants. (a) Condition of classification. As a condition of classification and...

  20. 8 CFR 1236.4 - Removal of S-5, S-6, and S-7 nonimmigrants.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 8 Aliens and Nationality 1 2011-01-01 2011-01-01 false Removal of S-5, S-6, and S-7 nonimmigrants... OF ALIENS ORDERED REMOVED Detention of Aliens Prior to Order of Removal § 1236.4 Removal of S-5, S-6, and S-7 nonimmigrants. (a) Condition of classification. As a condition of classification and...

  1. Transformations of the molecular complex of hydroiodide of 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene and 5,5‧-dibromo-2,2‧-biphenol between crystal and solutions

    NASA Astrophysics Data System (ADS)

    Wojciechowski, Grzegorz; Ratajczak-Sitarz, Małgorzata; Katrusiak, Andrzej; Brzezinski, Bogumil

    2005-05-01

    A complex of 5,5'-dibromo-2,2'-biphenol with 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene hydroiodide has been studied by X-ray diffraction, FT-IR, and 1HNMR spectroscopy. In the crystal structure, the iodide anion is hydrogen-bonded to the MTBDH + cation and to two hydroxyl groups of two 5,5'-dibromo-2,2'-biphenol molecules. In chloroform and acetonitrile the structure of the complex assumes a new structure due to almost complete dissociation of the protonated MTBD molecule. In chloroform the MTBDH + cation is hydrogen bonded to 5,5'-dibromo-2,2'-biphenol whereas in acetonitrile it is free and solvated by the solvent. In both cases the 5,5'-dibromo-2,2'-biphenol molecule is hydrogen bonded to the iodide anion. In chloroform the iodide ion interacts strongly with the solvent.

  2. 40 CFR 721.5262 - 2,7-Naphthalenedisulfonic acid, 5-[[4-chloro-6-[substituted] amino]-1,3,5-triazin-2-yl]amino]-4...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...,7-Naphthalenedisulfonic acid, 5- amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3- -, trisodium salt...-Naphthalenedisulfonic acid, 5- amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3- -, trisodium salt (PMN P-00-0803) is...-naphthalenedisulfonic acid, 5- ethyl]amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3- -, sodium salt (1:3) (PMN......

  3. 8 CFR 236.4 - Removal of S-5, S-6, and S-7 nonimmigrants.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 8 Aliens and Nationality 1 2011-01-01 2011-01-01 false Removal of S-5, S-6, and S-7 nonimmigrants... of Aliens Prior to Order of Removal § 236.4 Removal of S-5, S-6, and S-7 nonimmigrants. (a) Condition... section 101(a)(15)(S) of the Act, nonimmigrants in S classification must have executed Form I-854, Part...

  4. 8 CFR 236.4 - Removal of S-5, S-6, and S-7 nonimmigrants.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 8 Aliens and Nationality 1 2010-01-01 2010-01-01 false Removal of S-5, S-6, and S-7 nonimmigrants... of Aliens Prior to Order of Removal § 236.4 Removal of S-5, S-6, and S-7 nonimmigrants. (a) Condition... section 101(a)(15)(S) of the Act, nonimmigrants in S classification must have executed Form I-854, Part...

  5. Synthesis of 5,6-dihydro-4H-benzo[d]isoxazol-7-one and 5,6-dihydro-4H-isoxazolo[5,4-c]pyridin-7-one derivatives as potential Hsp90 inhibitors.

    PubMed

    Musso, Loana; Cincinelli, Raffaella; Giannini, Giuseppe; Manetti, Fabrizio; Dallavalle, Sabrina

    2015-11-01

    A novel class of 5,6-dihydro-4H-benzo[d]isoxazol-7-ones and 5,6-dihydro-4H-isoxazolo[5,4-c]pyridin-7-ones was designed, synthesized, and assayed to investigate the affinity toward Hsp90 protein. The synthetic route was based on a 1,3-dipolar cycloaddition of nitriloxides, generated in situ from suitable benzaldoximes, with 2-bromocyclohex-2-enones or 3-bromo-5,6-dihydro-1H-pyridin-2-ones. Whereas all the compounds bearing a benzamide group on the bicyclic scaffold were devoid of activity, the derivatives carrying a resorcinol-like fragment showed a remarkable inhibitory effect on Hsp90. Docking calculations were performed to investigate the orientation of the new compounds within the binding site of the enzyme. PMID:25855505

  6. 2-Amino-4-methyl-4,5,6,7-tetrahydro-1-benzothiophene-3-carbonitrile.

    PubMed

    Khan, Ashraf Y; Fathima, Nikhath; Kalashetti, Mallikarjun B; Begum, Noor Shahina; Khazi, I M

    2012-10-01

    In the title compound, C(10)H(12)N(2)S, the thio-phene ring is essentially planar (r.m.s. deviation = 0.0290 Å). The two C atoms of the cyclo-hexene ring (at positions 6 and 7) are disordered over two sets of sites in a 0.810 (5):0.190 (5) ratio. The cyclo-hexene rings in both the major and minor occupancy conformers adopt a half-chair conformation. In the crystal, there are two types of N-H⋯N inter-action. One of these results in centrosymmetric head-to-head dimers corresponding to an R(2) (2)(12) graph-set motif and the other forms a 20-membered macrocyclic ring involving six mol-ecules. PMID:23125792

  7. Crystal and molecular structure of a complex formed between the hydrochloride of 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene and 5,5'-dibromo-2,2'-biphenol

    NASA Astrophysics Data System (ADS)

    Bartoszak-Adamska, E.; Wojciechowski, G.; Jaskólski, M.; Brzezinski, B.

    2001-09-01

    A complex of 5,5'-dibromo-2,2'-biphenol with 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene (MTBD) hydrochloride has been studied using X-ray diffraction, FT-IR, and 1H NMR spectroscopy. The compound crystallises in the space group P2 1/ c with a=9.738(2), b=12.100(2), c=18.347(4) Å, β=92.62(3)° (at 100 K), and Z=4. In the crystal structure, the MTBDH + cation, a chloride anion, and one molecule of 5,5'-dibromo-2,2'-biphenol are present. In the solid state the Cl - anion is an acceptor in N +-H⋯Cl -, O-H⋯Cl - and C-H⋯Cl - intermolecular hydrogen bonds. In chloroform the structure of the complex is comparable with that in the solid, whereas in acetonitryle the complex assumes a new structure owing to the almost complete dissociation of the protonated MTBD molecule. The 5,5'-dibromo-2,2'-biphenol molecule is hydrogen-bonded with the chloride anion. Within this complex the OH⋯OH hydrogen bond shows very large proton polarisability indicated by the continuous absorption in the FTIR spectrum.

  8. Molecular structure of a complex formed between hydrobromide of 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene and 5,5'-dibromo-2,2'-biphenol in crystal and solutions

    NASA Astrophysics Data System (ADS)

    Wojciechowski, Grzegorz; Ratajczak-Sitarz, Małgorzata; Katrusiak, Andrzej; Brzezinski, Bogumil

    2002-08-01

    A complex of 5,5'-dibromo-2,2'-biphenol with 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene hydrobromide has been studied using X-ray diffraction, FT-IR, and 1H NMR spectroscopy. In the crystal structure, a bromide anion is hydrogen-bonded with MTBDH + cation and two hydroxyl groups of two 5,5'-dibromo-2,2'-biphenol molecules. In the solid state, the Br - anion is an acceptor in N +-H⋯Br -, and two O-H⋯Br - intermolecular hydrogen bonds. In chloroform and acetonitrile, the structure of the complex assumes a new structure due to almost complete dissociation of protonated MTBD molecule. In chloroform, the MTBDH + cation is hydrogen-bonded with 5,5'-dibromo-2,2'-biphenol whereas in acetonitrile it is free and solvated by the solvent. In both cases, the 5,5'-dibromo-2,2'-biphenol molecule is hydrogen-bonded to the bromide anion.

  9. IMMUNOHISTOCHEMICAL ANALYSIS OF ZNT1, 4, 5, 6, AND 7 IN THE MOUSE GASTROINTESTINAL TRACT

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Expression of five zinc transporters (ZnT1, 4, 5, 6, and 7) of the Slc30 family in the mouse gastrointestinal tract was studied by immunohistochemical analysis. The results demonstrated unique expression patterns, levels, and cellular localization among ZnT proteins in the mouse gastrointestinal tra...

  10. Molecular Mapping of Wheat: Major Genes and Rearrangements in Homoeologous Groups 4, 5, and 7

    PubMed Central

    Nelson, J. C.; Sorrells, M. E.; Van-Deynze, A. E.; Lu, Y. H.; Atkinson, M.; Bernard, M.; Leroy, P.; Faris, J. D.; Anderson, J. A.

    1995-01-01

    A molecular-marker linkage map of hexaploid wheat (Triticum aestivum L. em. Thell) provides a framework for integration with the classical genetic map and a record of the chromosomal rearrangements involved in the evolution of this crop species. We have constructed restriction fragment length polymorphism (RFLP) maps of the A-, B-, and D-genome chromosomes of homoeologous groups 4, 5, and 7 of wheat using 114 F(7) lines from a synthetic X cultivated wheat cross and clones from 10 DNA libraries. Chromosomal breakpoints for known ancestral reciprocal translocations involving these chromosomes and for a known pericentric inversion on chromosome 4A were localized by linkage and aneuploid analysis. Known genes mapped include the major vernalization genes Vrn1 and Vrn3 on chromosome arms 5AL and 5DL, the red-coleoptile gene Rc1 on 7AS, and presumptively the leaf-rust (Puccinia recondita f.sp. tritici) resistance gene Lr34 on 7DS and the kernel-hardness gene Ha on 5DS. RFLP markers previously obtained for powdery-mildew (Blumeria graminis f.sp. tritici) resistance genes Pm2 and Pm1 were localized on chromosome arms 5DS and 7AL. PMID:8647405

  11. Molecular design of new nitramine explosives: 1,3,5,7-tetranitro-8-(nitromethyl)-4-imidazolino[4,5-b]4-imidazolino-[4,5-e] pyridine and its N-oxide.

    PubMed

    Liu, Hui; Du, Hongchen; Wang, Guixiang; Liu, Yan; Gong, Xuedong

    2012-04-01

    Two new nitramine compounds containing pyridine, 1,3,5,7-tetranitro-8-(nitromethyl) -4-imidazolino[4,5-b]4-imidazolino-[4,5-e]pyridine and its N-oxide 1,3,5,7-tetranitro-8- (nitromethyl)-4-imidazolino[4,5-b]4-imidazolino-[4,5-e]pyridine-4-ol were proposed. Density functional theory (DFT) has been employed to study the molecular geometries, electronic structures, infrared spectra, and thermodynamic properties at the B3LYP/6-31G* level. Their detonation performances evaluated using the Kamlet-Jacobs equations with the calculated densities and heats of formation are superior to those of HMX. The predicted densities of them were ca. 2 g cm(-3), detonation velocities were over 9 km s(-1), and detonation pressures were about 40 GPa, showing that they may be potential candidates of high energy density materials (HEDMs). The natural bond orbital analysis indicated that N-NO(2) bond is the trigger bond during thermolysis process. The stability of the title compounds is slightly lower than that of 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12- hexaazaisowurtzitane (CL-20). The results of this study may provide basic information for the molecular design of new HEDMs. PMID:21748326

  12. Cooperative hydrogen-bonded chain in molecular ionic complex of 5,5'-dibromo-2,2'-biphenol with 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene hydrofluoride

    NASA Astrophysics Data System (ADS)

    Wojciechowski, Grzegorz; Ratajczak-Sitarz, Małgorzata; Katrusiak, Andrzej; Brzezinski, Bogumil

    2002-08-01

    A complex of 5,5'-dibromo-2,2'-biphenol (DBBPh) with 7-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene (MTBD) hydrofluoride has been studied using X-ray diffraction, FT-IR, and 1H NMR spectroscopy. The formula unit of the crystal structure consists of a MTBDH + cation, a fluoride anion, and one neutral molecule of 5,5'-dibromo-2,2'-biphenol. In the solid state the F - anion is an acceptor in two very short O-H⋯F - intermolecular hydrogen bonds of 2.487 and 2.390 Å involving the hydroxyl groups. One oxygen atom of these groups forms also an N +-H⋯O intermolecular hydrogen bond of 2.775 Å with the MTBDH + cation. In chloroform and acetonitrile, the structures of the complexes are comparable with those studied previously for 5,5'-dibromo-2,2'-biphenol with MTBD, because the hydrogen fluoride goes into the gas phase during the solution process.

  13. Airborne spectrophotometry of Eta Carinae from 4.5 to 7.5 microns and a model for source morphology

    NASA Technical Reports Server (NTRS)

    Russell, Ray W.; Lynch, David K.; Hackwell, John A.; Rudy, Richard J.; Rossano, George S.; Castelaz, M. W.

    1987-01-01

    Spectrophotometric observations of Eta Car between 4.5 and 7.5 microns show a featureless thermal-like spectrum with no fine-structure lines or broad emission or absorption features. The color temperature of the spectrum is approximately 375 K. High spatial resolution maps at 3.5, 4.8, and 10 microns obtained from the ground are used to discuss the dust distribution and temperature structure, and to present a model for general source morphology. The upper limit to the brightness of the forbidden Ar II fine-structure emission line at 6.98 microns is less than 7 x 10 to the -16th W/sq cm, which still allows for a significant overabundance of argon and is consistent with the evolved nature of the source.

  14. Carboxyl-terminal fusion of E7 into Flagellin shifts TLR5 activation to NLRC4/NAIP5 activation and induces TLR5-independent anti-tumor immunity

    PubMed Central

    Lin, Kuo-Hsing; Chang, Li-Sheng; Tian, Chun-Yuan; Yeh, Yi-Chen; Chen, Yu-Jie; Chuang, Tsung-Hsien; Liu, Shih-Jen; Leng, Chih-Hsiang

    2016-01-01

    Flagellin has the capacity to activate both Toll-like receptor 5 (TLR5) and Nod-like receptor C4 (NLRC4)/neuronal apoptosis inhibitory protein 5 (NAIP5) inflammasome signaling. We fused E7m (the inactivated E7 of human papillomavirus) to either end of the flagellin protein, and the resulting recombinant flagellin-E7m proteins (rFliCE7m and rE7mFliC) were used as immunogens. Both fusion proteins activated receptor signaling to different degrees. rE7mFliC-induced TLR5 activity was 10-fold higher than that of rFliCE7m, whereas rFliCE7m activated the NLRC4/NAIP5 pathway more strongly. Therefore, these recombinant proteins provided a tool to investigate which signaling pathway is critical for the induction of antigen-specific T cell responses and anti-tumor immunity. We demonstrated that rFliCE7m induced higher levels of E7-specific IFN-gamma-secreting cells and cytotoxic T lymphocytes (CTLs) than rE7mFliC, and a single injection with rFliCE7m but not rE7mFliC inhibited E7-expressing tumor growth in vivo. Furthermore, we confirmed that CD8+ T cells played a major role in the anti-tumor immunity induced by rFliCE7m. These findings suggested that the NLRC4/NAIP5 intracellular signaling pathway was critical for the induction of anti-tumor immunity. These observations provide important information for the rational design of flagellin-based immunotherapy. PMID:27063435

  15. Synthesis and characterization of Ni(II) complex with 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-7,14-dienium bromide

    SciTech Connect

    Yusoff, Latifah M.; Yusoff, Siti Fairus M.; Ismail, Wafiuddin; Yamin, Bohari M.

    2014-09-03

    Nickel(II) complex have been synthesized by treating a 14-membered ring tetraaza macrocyclic compound, 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-7,14-dienium, bromide (Me{sub 6}N{sub 4}H{sub 4})Br{sub 2} with nickel acetate in metanol. The complex was characterized using elemental analysis, Fourier Transform Infrared (FTIR), Ultraviolet-Visible (UV-Vis), and single crystal diffraction (X-ray). The nickel atom coordinates through four nitrogen atoms in the ligand. Square planar geometry has been proposed for this complex.

  16. High antiallergic activity of 5,6,4'-trihydroxy-7,8,3'-trimethoxyflavone and 5,6-dihydroxy-7,8,3',4'-tetramethoxyflavone from eau de cologne mint (Mentha×piperita citrata).

    PubMed

    Sato, Akihiko; Tamura, Hirotoshi

    2015-04-01

    The following compounds with higher antiallergic activities were isolated from eau de cologne mint leaves: 5,6,4'-trihydroxy-7,8-dimethoxyflavone (6), 5,6,4'-trihydroxy-7,8,3'-trimethoxyflavone (7), 5,6-dihydroxy-7,3',4'-trimethoxyflavone (8), 5,6-dihydroxy-7,8,3',4'-tetramethoxyflavone (9), and 5,6-dihydroxy-7,8,4'-trimethoxyflavone (10). The IC50 values of compounds 6-10 against RBL-2H3 cells were 6.7, 2.4, 5.6, 3.0, and 6.1μM. Compounds 7 and 9 (IC50 2.4μM and 3.0μM) had the highest antiallergic activities among the flavonoids previously reported. The amounts of 7, 9, and 10 isolated were fairly high, at 177.7mg/kg, 278.0mg/kg, and 179.7mg/kg in the mint, respectively. LD5 value (index of toxicity) and LD5/IC50 ratio of 7 and 9 indicate that the safety is greater than that of luteolin, a typical antiallergic substance. The extract containing powerful antiallergic flavones, 6-10 with higher hydrophobicity could be selectively separated from the extract containing luteolin and other flavonoid glycosides by partition with dichloromethane and water. Therefore, compounds 7 and 9 in mint, and the dichloromethane extract would be the most potent and preventive resources against type I allergy. PMID:25704366

  17. Facile Synthesis and NO-Generating Property of 4H-[1,2,5]Oxadiazolo[3,4-d]pyrimidine-5,7-dione 1-Oxides.

    PubMed

    Sako, Magoichi; Oda, Souichi; Ohara, Seiji; Hirota, Kosaku; Maki, Yoshifumi

    1998-10-01

    4H-[1,2,5]Oxadiazolo[3,4-d]pyrimidine-5,7-dione 1-oxides (2) are conveniently prepared in high yields by the oxidative intramolecular cyclization of 6-amino-5-nitro-1H-pyrimidine-2,4-diones (1) employing iodosylbenzene diacetate as an oxidant in the presence of lithium hydride. The generation of nitric oxide (NO) and NO-related species from 2 occurs in the presence of thiols such as N-acetylcysteamine, cysteine, and glutathione under physiological conditions. The evidence for the NO generation derives from mechanistic interpretations for the reaction of 2 with thiols and other chemical observations. PMID:11672316

  18. Complexation of 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-7,14-dienium bromide with copper salts

    NASA Astrophysics Data System (ADS)

    Huddin, Ameera Aqeela Salleh; Yamin, Bohari M.; Yusoff, Siti Fairus M.

    2014-09-01

    Copper complexes have been synthesized with 14-membered macrocyclic 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-7,14-dienium bromide (Me6N4H4)Br2 as ligand having six methyl groups and two double bonds located in diagonal position. These complexes were characterized by microelemental analysis, Fourier Transform Infrared (FTIR) and Ultraviolet-Visible (UV-VIS) spectroscopies. The chemical analysis and spectroscopic data is supported by chemical crystallographic study showed that one of the synthesized copper complex with CuCl2 consists of [Cu(Me6N4H2)] dication, two bromide counter anions and 2 water molecules of crystallisation. Thermogravimetry Analysis (TGA) showed that the complex is stable up to about 200°C before gradually decomposed up to 400°C.

  19. Microstructure, thermal, and mechanical properties of nanostructured Cu-9.5Ni-4.0Sn-7.5P

    NASA Astrophysics Data System (ADS)

    Li, J.; Wang, T. M.

    1995-04-01

    Nanostructured Cu-9.5Ni-4.0Sn-7.5P samples represent a polycrystal microstructure of nanometer-sized α-Cu and Cu3P crystallites for crystallite sizes less than 20 nm and of nanometer-sized α-Cu, Cu3P, and Ni2P crystallites for crystallite sizes greater than 20 nm. The specific heat values between 300 K and 400 K for the nanostructured sample with crystallite size of 10 nm are about 20% higher than for the amorphous sample and about 40% higher than for the coarse-grained sample. The hardness of the nanostructured sample with crystallite size of 10 nm is 30% higher than that of the amorphous sample and 110% higher than that of the coarse-grained sample. The variation in hardness with the crystallite size for the nanostructured samples follows the Hall-Petch relationship.

  20. Complete genome sequence of the marine, cellulose and xylan degrading bacterium Glaciecola sp. 4H-3-7+YE-5

    SciTech Connect

    Klippel, Dr Barbara; Bruce, David; Davenport, Karen W.; Goodwin, Lynne A.; Han, James; Han, Shunsheng; Land, Miriam L; Mikhailova, Natalia; Nolan, Matt; Pennacchio, Len; Pitluck, Sam; Tapia, Roxanne; Woyke, Tanja; Wiebusch, Sigrid; Basner, Alexander; Abe, Fumiyoshi; Horikoshi, Koki; Antranikian, Garabed

    2011-01-01

    Glaciecola sp. 4H-3-7+YE-5 was isolated from deep sea sediments at Suruga Bay in Japan and is capable of efficiently hydrolyzing cellulose and xylan. The complete genome sequence of Glaciecola sp. 4H-3-7+YE-5 revealed several genes encoding putatively novel glycoside hydrolases associated with plant biomass degradation.

  1. Comparison of laser ablation and sputter desorption of clusters from Au7Cu5Al4

    NASA Astrophysics Data System (ADS)

    King, B. V.; Moore, J. F.; Cui, Y.; Veryovkin, I. V.; Tripa, C. E.

    2014-12-01

    Ionized and neutral clusters were desorbed from spangold, a polycrystalline ternary alloy with composition Au7Cu5Al4, using both a femtosecond laser beam and an energetic ion beam and the resulting time of flight mass spectra compared. Neutral clusters containing up to 7 atoms were ejected by the 15 keV Ar+ beam whereas only smaller positively and negatively charged clusters were observed from the laser ablated spangold surface. Laser ionization mass spectrometry (LIMS) positive ion spectra were dominated by Al containing cluster ions whereas Au containing ions dominated the negative LIMS spectrum. An odd-even variation in LIMS cluster yield was observed, consistent with previous results and due to fragmentation of photoionized clusters. The laser sputtered neutral mass spectrometry (laser SNMS) spectrum showed that larger desorbed clusters were gold rich. The cluster signals also followed a power law dependence with cluster size with the exponent value of 6-7.6 for sputtered mixed clusters being greater than that found from sputtering of pure elements, similar to the result found previously in the Cu-Au system.

  2. Synthesis, structures, electrochemical studies and antioxidant activity of 5-aryl-4-oxo-3,4,5,8-tetrahydropyrido[2,3-d]pyrimidine-7-carboxylic acids

    NASA Astrophysics Data System (ADS)

    Quiroga, Jairo; Romo, Pablo E.; Ortiz, Alejandro; Isaza, José Hipólito; Insuasty, Braulio; Abonia, Rodrigo; Nogueras, Manuel; Cobo, Justo

    2016-09-01

    The synthesis of 5-aryl-4-oxo-3,4,5,8-tetrahydropyrido[2,3-d]pyrimidine-7-carboxylic acids 3 from the reaction of 6-aminopyrimidines 1 with arylidene derivatives of pyruvic acid 2 under microwave and ultrasound irradiation is described. The orientation of cyclization process was determined by NMR measurements. The methodology provides advantages such as high yields and friendly to the environment without the use of solvents. The antioxidant properties, DPPH free radical scavenging, ORAC, and anodic potential oxidation of the new pyridopyrimidines were studied.

  3. New composite spectra of Mars, 0.4-5.7 μm

    USGS Publications Warehouse

    Erard, Stephane; Calvin, Wendy M.

    1997-01-01

    About 15 areas were observed in the equatorial regions of Mars by the infrared spectrometers IRS (Mariner 6 and 7) and ISM (Phobos-2). The comparison between the spectra shows a remarkable consistency between two data sets acquired 20 years apart and calibrated independently. This similarity demonstrates the accuracy of ISM calibration above 2 μm, except for a possible stray light contribution above 2.6 μm, on the order of ∼1–2% of the solar flux at 2.7 μm. Most differences in spectral shapes are related to differences in spectral/spatial resolution and viewing geometries. No important variation in surface properties is detected, except for a spot in southern Arabia Terra which has a much deeper hydration feature in IRS spectra; differences in viewing geometries and spatial resolutions do not seem to account for this difference that could result from shifting or dehydration of surface materials. Composite spectra of several types of bright and dark materials are computed by modeling the thermal emission and are completed with telescopic spectra in the visible range. Modeled reflectance in the 3.0–5.7 μm range is consistent with basalts and palagonites. The bright regions and analog palagonite spectra are different from hematite in this range, but resemble several phyllosilicates. We infer that (1) although hematite dominates the spectra in the 0.4- to 2.5-μm range, the silicate-clay host is spectrally active beyond 3 μm and can be identified from this domain; (2) phyllosilicates such as montmorillonite or smectite may be abundant components of the martian soils, although the domain below 3 μm lacks the characteristic features of the most usual terrestrial clay minerals.

  4. Synthesis and anticancer activities of 4-(4-substituted piperazin)-5,6,7-trialkoxy quinazoline derivatives.

    PubMed

    Zhang, Ying; Huang, Yin-Jiu; Xiang, Hong-Mei; Wang, Pei-Yi; Hu, De-Yu; Xue, Wei; Song, Bao-An; Yang, Song

    2014-05-01

    A series of 4-(4-substituted piperazin)-5,6,7-trialkoxy quinazoline was prepared by conventional heating methods. Among these compounds, the crystal structure of compound 10o (CCDC: 916922) was determined by X-ray crystallography. Bioassay results showed that most target compounds had certain inhibition activities against proliferation of tumor cells, and some compounds even had good broad-spectrum inhibition activities. The ethoxyl series of compounds possessed higher inhibition activities against tumor cells than the methoxyl series of compounds. Bioactivity tests showed that the IC50 values of compound 10s against PC3, MGC803, A375, and A549 cells were 1.8, 2.8, 1.3, and 2.9 μΜ, respectively, which were much higher than those of commercial gefitinib (7.2, 7.6, 7.2, and 9.8 μM, respectively). Conversely, the IC50 values of compound 10s were very low against NH3T3, indicating only weak effect on normal cells as also proven by lactate dehydrogenase and acridine orange/ethidium bromide staining. Analyses of cell configuration and cell cycle revealed that compound 10s possibly caused cells to remain at G0/G1 phase by inhibiting cell proliferation for 24 h. Compound 10s also inhibited the phosphorylation of ERK1/2 and P38 with obvious concentration dependence. Thus, these compounds can inhibit the proliferation of A549 cells through the interruption of ERK1/2 and P38signaling pathways. PMID:24675177

  5. (3-Methyl-3a,4,7,7a-tetra­hydro-5H-4,7-methano­isoxazolo[4,5-d][1,2]oxazin-5-yl)(phen­yl)methanone

    PubMed Central

    Lough, Alan J.; Nagireddy, Jaipal R.; Tam, William

    2014-01-01

    The title compound, C14H14N2O3, is the exo isomer with a syn arrangement of two O atoms in the isoxazole and oxazine rings. The dihedral angle between the isoxazole and phenyl rings is 60.38 (4)°. In the crystal, weak C—H⋯O hydrogen bonds link the mol­ecules, forming a three-dimensional network. The isoxazole O atom is an acceptor for three of these hydrogen bonds. PMID:24860351

  6. Crystal structure of (±)-(7RS,8SR)-7-methyl-1,4-dioxa-spiro-[4.5]decane-7,8-diol.

    PubMed

    Oishi, Takeshi; Yamamoto, Hiroaki; Sugai, Tomoya; Fukaya, Keisuke; Yamaguchi, Yu; Watanabe, Ami; Sato, Takaaki; Chida, Noritaka

    2015-10-01

    In the title compound, C9H16O4, the five-membered dioxolane ring adopts a twist conformation; two adjacent C atoms deviate alternately from the mean plane of other atoms by -0.297 (4) and 0.288 (4) Å. The spiro-fused cyclo-hexane ring shows a chair form. The hy-droxy group substituted in an axial position makes an intra-molecular O-H⋯O hydrogen bond with one of the O atoms in the cyclic ether, forming an S(6) ring motif. In the crystal, the O-H⋯O hydrogen bond involving the equatorial hy-droxy group connects the mol-ecules into a zigzag chain with a C(5) motif running along the c axis. PMID:26594401

  7. Complete Genome Sequences of Krokinobacter sp. 4H-3-7-5 and Lacinutrix sp. 5H-3-7-4, polysaccharide-degrading members of the family Flavobacteriaceae

    SciTech Connect

    Klippel, Dr Barbara; Bruce, David; Davenport, Karen W.; Detter, J C; Goodwin, Lynne A.; Han, James; Han, Shunsheng; Land, Miriam L; Nolan, Matt; Ovchinnikova, Galina; Pennacchio, Len; Pitluck, Sam; Tapia, Roxanne; Walston Davenport, Karen; Woyke, Tanja; Wiebusch, Sigrid; Basner, Alexander; Abe, Fumiyoshi; Horikoshi, Koki; Antranikian, Garabed

    2011-01-01

    Two members of the family Flavobacteriaceae were isolated from deep sea sediments in Japan using artificial seawater and cellulose, xylan and chitin as sole carbon and energy source. Here, we present the finished genome sequence of Krokinobacter sp. 4H-3-7-5 and Lacinutrix sp. 5H-3-7-4 which both encode for putatively novel enzymes involved in cellulose, hemicellulose and chitin degradation.

  8. Synthesis, characterization, and crystal structure of 2-amino-7-methyl-5-oxo-4-phenyl-4,5-dihydropyrano[3,2-c] pyran-3-carbonitrile

    SciTech Connect

    Sharma, S.; Banerjee, B.; Brahmachari, G.; Kant, Rajni; Gupta, V. K.

    2015-12-15

    2-Amino-7-methyl-5-oxo-4-phenyl-4,5-dihydropyrano[3,2-c] pyran-3-carbonitrile, C{sub 16}H{sub 12}N{sub 2}O{sub 3} is synthesized via one-pot multi-component reaction at room temperature using commercially available urea as inexpensive and environmentally benign organo-catalyst. Its structure is determined by single-crystal X-ray diffraction technique The crystals are monoclinic, a = 10.7357(12), b = 8.7774(8), c = 15.0759(16) Å, β = 103.575(11)°, Z = 4, sp. gr. P2{sub 1}/n, R = 0.0551 for 1696 observed reflections. The crystal structure is stabilized by N–H···N, C–H···O, and C–H···π interactions.

  9. Synthesis, characterization, and crystal structure of 2-amino-7-methyl-5-oxo-4-phenyl-4,5-dihydropyrano[3,2- c]pyran-3-carbonitrile

    NASA Astrophysics Data System (ADS)

    Sharma, S.; Banerjee, B.; Brahmachari, G.; Kant, Rajni; Gupta, V. K.

    2015-12-01

    2-Amino-7-methyl-5-oxo-4-phenyl-4,5-dihydropyrano[3,2- c]pyran-3-carbonitrile, C16H12N2O3 is synthesized via one-pot multi-component reaction at room temperature using commercially available urea as inexpensive and environmentally benign organo-catalyst. Its structure is determined by single-crystal X-ray diffraction technique The crystals are monoclinic, a = 10.7357(12), b = 8.7774(8), c = 15.0759(16) Å, β = 103.575(11)°, Z = 4, sp. gr. P21/ n, R = 0.0551 for 1696 observed reflections. The crystal structure is stabilized by N-H···N, C-H···O, and C-H···π interactions.

  10. Design, Synthesis and Biological Evaluation of 5-Oxo-1,4,5,6,7,8 Hexahydroquinoline Derivatives as Selective Cyclooxygenase-2 Inhibitors

    PubMed Central

    Zarghi, Afshin; Sabakhi, Iman; Topuzyan, Vigen; Hajimahdi, Zahra; Daraie, Bahram

    2014-01-01

    A group of regioisomeric 5-oxo-1,4,5,6,7,8 hexahydroquinoline derivatives possessing a COX-2 SO2Me pharmacophore at the para position of the C-2 or C-4 phenyl ring, in conjunction with a C-4 or C-2 phenyl (4-H) or substituted-phenyl ring (4-F,4-Cl,4-Br,4-OMe,4-Me, 4-NO2), were designed for evaluation as selective cyclooxygenase-2 (COX-2) inhibitors. These target 5-oxo-1,4,5,6,7,8 hexahydroquinolines were synthesized via a Hansch condensation reaction. In vitro COX-1/COX-2 isozyme inhibition structure-activity studies identified 7,8-dihydro- 7,7-dimethyl-2-(4-methoxyphenyl)-4-(4-(methylsulfonyl)phenyl)quinolin-5(1H,4H,6H)- one (9c) as a potent COX-2 inhibitor (IC50 = 0.17 M) with a high COX-2 selectivity index (S.I. = 97.6) comparable to the reference drug celecoxib (COX-2 IC50 = 0.05 mM; COX-2 S.I= 405). A molecular modeling study where 9c was docked in active site of COX-2 showed that the p-SO2Me substituent on the C-2 phenyl ring is inserted into the secondary COX-2 binding site. The structure activity data acquired indicate that the position of the COX-2 SO2Me pharmacophore and type of substituent are important for COX-2 inhibitory activity. PMID:24711830

  11. 4 CFR 7.5 - Adverse actions: Suspensions for 14 days or less.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Section 7.5 Accounts GOVERNMENT ACCOUNTABILITY OFFICE PERSONNEL SYSTEM PERSONNEL RELATIONS AND SERVICES... writing and to furnish affidavits and other documentary evidence in support of the answer; (3) Be... therefor, and any order effecting the suspension, together with any supporting material, shall...

  12. Anti-platelet activity of erythro-(7S,8R)-7-acetoxy-3,4,3',5'-tetramethoxy-8-O-4'-neolignan from Myristica fragrans.

    PubMed

    Kang, Jung Won; Min, Byung-Sun; Lee, Jeong-Hyung

    2013-11-01

    Platelets play a critical role in pathogenesis of cardiovascular disorders and strokes. The inhibition of platelet function is beneficial for the treatment and prevention of these diseases. In this study, we investigated the anti-platelet activity of erythro-(7S,8R)-7-acetoxy-3,4,3',5'-tetramethoxy-8-O-4'-neolignan (EATN), a neolignan isolated from Myristica fragrans, using human platelets. EATN preferentially inhibited thrombin- and platelet-activating factor (PAF)-induced platelet aggregation without affecting platelet damage in a concentration-dependent manner with IC50 values of 3.2 ± 0.4 and 3.4 ± 0.3 μM, respectively. However, much higher concentrations of EATN were required to inhibit platelet aggregation induced by arachidonic acid. EATN also inhibited thrombin-induced serotonin and ATP release, and thromboxane B2 formation in human platelets. Moreover, EATN caused an increase in cyclic AMP (cAMP) levels and attenuated intracellular Ca(2+) mobilization in thrombin-activated human platelets. Therefore, we conclude that the inhibitory mechanism of EATN on platelet aggregation may increase cAMP levels and subsequently inhibit intracellular Ca(2+) mobilization by interfering with a common signaling pathway rather than by directly inhibiting the binding of thrombin or PAF to their receptors. This is the first report of the anti-platelet activity of EATN isolated from M. fragrans. PMID:23296979

  13. Synthesis and Crystal Structures of Hg 6Sb 5Br 7, Hg 6As 4BiCl 7, and Hg 6Sb 4BiBr 7, Built of a Polycationic Mercury-Pnictide Framework with Trapped Anions

    NASA Astrophysics Data System (ADS)

    Beck, Johannes; Hedderich, Sylvia; Neisel, Udo

    2000-11-01

    Hg6Sb5Br7, Hg6As4BiCl7, and Hg6Sb4BiBr7 were prepared from stoichiometric mixtures of Hg2X2, HgX2 (X=Cl, Br), As, Sb, and Bi in sealed, evacuated glass ampoules in temperature gradients 260→240°C for Hg6Sb5Br7, 340→320°C for Hg6As4BiCl7, and 290→270°C for Hg6Sb4BiBr7. All compounds crystallize in the cubic space group Paoverline3 with Z=4 and the lattice constants a=13.003(1) Å for Hg6Sb5Br7, a=12.178(2) Å for Hg6As4BiCl7, and a=12.998(4) Å for Hg6Sb4BiBr7. The structures have been solved based on single-crystal X-ray diffraction data and refined to R(F)=0.0431, 666 Fo for Hg6Sb5Br7, R(F)=0.0478, 690 Fo for Hg6As4BiCl7, and R(F)=0.0444, 840 Fo for Hg6Sb4BiBr7 with 30 parameters for each refinement. The structures are characterized by a three-dimensional polycationic framework of pnictide dumb-bells (As-As distance 2.43 Å, Sb-Sb distance 2.78 Å), each connected by six mercury atoms to six neighbored As2/Sb2 groups. There are two different cages in the framework; one type is occupied by nearly regular MX6 octahedra (M=Sb,Bi; X=Cl, Br), the other by halide ions. The three compounds crystallize closely related to Cd7P4Cl6, which contains a similar polycationic framework of P2 dumb-bells connected by Cd, but with only one type of cage occupied by octahedral [CdCl6]4- ions. The interactions between the atoms of the polycationic framework and the anions are very weak. The observed diamagnetism of all three compounds is in agreement with the ionic formulas (Hg6Sb4)4+[SbBr6]3-Br-, (Hg6As4)4+[BiCl6]3-Cl-, and (Hg6Sb4)4+[BiBr6]3-Br-.

  14. Metabolism of 4-Hydroxy-7-oxo-5-heptenoic Acid (HOHA) Lactone by Retinal Pigmented Epithelial Cells.

    PubMed

    Wang, Hua; Linetsky, Mikhail; Guo, Junhong; Yu, Annabelle O; Salomon, Robert G

    2016-07-18

    4-Hydroxy-7-oxo-5-heptenic acid (HOHA)-lactone is a biologically active oxidative truncation product released (t1/2 = 30 min at 37 °C) by nonenzymatic transesterification/deacylation from docosahexaenoate lipids. We now report that HOHA-lactone readily diffuses into retinal pigmented epithelial (RPE) cells where it is metabolized. A reduced glutathione (GSH) Michael adduct of HOHA-lactone is the most prominent metabolite detected by LC-MS in both the extracellular medium and cell lysates. This molecule appeared inside of ARPE-19 cells within seconds after exposure to HOHA-lactone. The intracellular level reached a maximum concentration at 30 min and then decreased with concomitant increases in its level in the extracellular medium, thus revealing a unidirectional export of the reduced GSH-HOHA-lactone adduct from the cytosol to extracellular medium. This metabolism is likely to modulate the involvement of HOHA-lactone in the pathogenesis of human diseases. HOHA-lactone is biologically active, e.g., low concentrations (0.1-1 μM) induce secretion of vascular endothelial growth factor (VEGF) from ARPE-19 cells. HOHA-lactone is also a precursor of 2-(ω-carboxyethyl)pyrrole (CEP) derivatives of primary amino groups in proteins and ethanolamine phospholipids that have significant pathological and physiological relevance to age-related macular degeneration (AMD), cancer, and wound healing. Both HOHA-lactone and the derived CEP can contribute to the angiogenesis that defines the neovascular "wet" form of AMD and that promotes the growth of tumors. While GSH depletion can increase the lethality of radiotherapy, because it will impair the metabolism of HOHA-lactone, the present study suggests that GSH depletion will also increase levels of HOHA-lactone and CEP that may promote recurrence of tumor growth. PMID:27355557

  15. Relaxant mechanisms of 3, 5, 7, 3', 4'-pentamethoxyflavone on isolated human cavernosum.

    PubMed

    Jansakul, Chaweewan; Tachanaparuksa, Kuldej; Mulvany, Michael J; Sukpondma, Youwapa

    2012-09-15

    We have investigated effects and mechanisms responsible for the activity of 3, 5, 7, 3', 4'-pentamethoxyflavone (PMF) on isolated human cavernosum. PMF is the major flavone isolated from Kaempferia parviflora claimed to act as an aphrodisiac. PMF caused relaxation of phenylephrine precontracted human cavernosal strips, and this effect was slightly inhibited by N(G)-nitro-l-arginine, a nitric oxide synthase inhibitor, but not by ODQ (soluble guanylate cyclase inhibitor), TEA (tetraethylammonium, blocker of voltage-dependent K(+) channels) or glybenclamide (blocker of ATP-dependent K(+) channels). PMF did not significantly inhibit the relaxant activity of glyceryltrinitrate or acetylcholine on human cavernosal strips precontracted with phenylephrine. In contrast, sildenafil (phosphodiesterase inhibitor) potentiated the relaxant activity of glyceryl trinitrate but not of acetylcholine. In normal Krebs solution with nifedipine (blocker of l-type Ca(2+) channels), or in Ca(2+)-free Krebs solution, PMF caused a further inhibition of human cavernosum contracted with phenylephrine. In human cavernosum treated with thapsigargin (inhibitor of sarcoplasmic reticulum Ca(2+)-ATPase) in Ca(2+)-free medium, PMF suppressed the concentration-response curve of human cavernosum to phenylephrine and a further suppression was found when SKF-96365 (a blocker of store-operated Ca(2+) channels and Y-27632 (inhibitor of Rho-kinase)), but not nifedipine, were added sequentially. Thus, PMF had only a weak effect on the release of nitric oxide, and had no effect as a K(ATP)- or K(Ca) channel opener, a phosphodiesterase inhibitor, a store-operated Ca(2+) channel blocker or a Rho-kinase inhibitor. Therefore, these studies suggest that PMF causes relaxation of human cavernosum through voltage-dependent Ca(2+) channels and other mechanisms associated with calcium mobilization. PMID:22800934

  16. 3,5,7-Trimeth­oxy-2-(4-methoxy­phen­yl)-4H-1-benzopyran-4-one

    PubMed Central

    Aree, Thammarat; Sawasdee, Pattara

    2009-01-01

    In the title compound, C19H18O6, also known as 3,4′,5,7-tetra­methoxy­flavone, the dihedral angle between the benzopyran-4-one group and the attached benzene ring is 11.23 (8)°. An intra­molecular C—H⋯O hydrogen bond generates an S(6) ring motif. In the crystal, mol­ecules are linked into a two-dimensional network parallel to (01) by inter­molecular C—H⋯O hydrogen bonds, which generate R 4 4(20), R 4 4(12) and R 2 2(14) ring motifs. Adjacent networks interact by π–π inter­actions between the pyran ring and its methoxy­phenyl substituent [centroid–centroid distance = 3.5267 (8) Å]. PMID:21578306

  17. Synergistic effect of 2,2',4,4',5,5'-hexachlorobiphenyl and 2,3,7,8-tetrachlorodibenzo-p-dioxin on hepatic porphyrin levels in the rat.

    PubMed Central

    van Birgelen, A P; Fase, K M; van der Kolk, J; Poiger, H; Brouwer, A; Seinen, W; van den Berg, M

    1996-01-01

    We studied the effect of polychlorinated biphenyls (PCBs) on hepatic porphyrin accumulation in female Sprague-Dawley rats by feeding them diets containing 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,2',4,4',5,5'-hexachlorobiphenyl (PCB 153), 2,3,3',4,4',5-hexachlorobiphenyl (PCB 156), 3,3',4,4',5-pentachlorobiphenyl (PCB 126), or combinations of the single PCB congeners with TCDD for 13 weeks. A dose-dependent increase in hepatic porphyrin accumulation occurred after TCDD, PCB 126, or PCB 156 administration, reaching maximal levels of about twice control values. The lowest dose levels for which a significant increase in hepatic porphyrin accumulation was found were 0.7 microgram TCDD/kg diet, 50 micrograms PCB 126/kg diet, or 6 mg PCB 156/kg diet. These doses are equivalent to 47 ng TCDD/kg/day, 3.2 micrograms PCB 126/kg/day, and 365 micrograms PCB 156/kg/day. Relative potencies for hepatic porphyrin accumulation, using TCDD as a reference, ranged from 0.015 to 0.06 for PCB 126 and from 0.0001 to 0.0003 for PCB 156. CYP1A2 activities significantly correlated with hepatic porphyrin levels, with coefficients of 0.629, 0.483, or 0.808 for TCDD, PCB 126, or PCB 156, respectively. Administration of PCB 153 alone did not result in hepatic porphyrin accumulation. Co-administration of PCB 153 and TCDD revealed a strong synergistic effect on porphyrin accumulation (about 800 times control levels). This synergistic effect was significant in rats fed diets containing any combination of PCB 153 with TCDD. Uroporphyrin III and heptacarboxylic porphyrin were accumulated in porphyrinogenic livers. These results suggest that TCDD induction of CYP1A2 may be involved, leading to oxidation of uroporphyrinogen III to uroporphyrin III, in combination with an increase in delta-aminolevulinic acid synthetase induced by PCB 153. Under porphyrinogenic conditions, an inhibitor of CYP1A2 activity may also be formed. The interactive effects on porphyrin accumulation after co

  18. Process for manufacturing bis(2-methoxyethyl)-2,3,6,7-tetracyano-1,4,5,8,9,10-hexazaanthracene

    SciTech Connect

    Rasmussen, Paul George; Lawton, Richard Graham

    2014-06-03

    A process to manufacture substituted tetracyano-hexaazatricyclics with the substitutions occurring at the 9 and 10 hydrogens. The process begins with 2,3-dichloro-5,6-dicyanopyrazine, which is reacted to form the desired tetracyano-hexaazatricyclic. Different process embodiments enable different reaction paths to the desired tetracyano-hexaazatricyclic. Different tetracyano-hexaazatricyclic embodiments include bis(2-methoxyethyl)-2,3,6,7-tetracyano-1,4,5,8,9,10-hexazaanthracene and bis(2-methoxyethoxyethyl)-2,3,6,7-tetracyano-1,4,5,8,9,10-hexazaanthracen- e.

  19. 47 CFR 2.108 - Policy regarding the use of the fixed-satellite allocations in the 3.6-3.7, 4.5-4.8, and 5.85-5...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 1 2011-10-01 2011-10-01 false Policy regarding the use of the fixed-satellite... Allocation, Assignment, and Use of Radio Frequencies § 2.108 Policy regarding the use of the fixed-satellite allocations in the 3.6-3.7, 4.5-4.8, and 5.85-5.925 GHz bands. The use of the fixed-satellite allocations...

  20. 47 CFR 2.108 - Policy regarding the use of the fixed-satellite allocations in the 3.6-3.7, 4.5-4.8, and 5.85-5...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 1 2012-10-01 2012-10-01 false Policy regarding the use of the fixed-satellite... Allocation, Assignment, and Use of Radio Frequencies § 2.108 Policy regarding the use of the fixed-satellite allocations in the 3.6-3.7, 4.5-4.8, and 5.85-5.925 GHz bands. The use of the fixed-satellite allocations...

  1. 47 CFR 2.108 - Policy regarding the use of the fixed-satellite allocations in the 3.6-3.7, 4.5-4.8, and 5.85-5...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 1 2013-10-01 2013-10-01 false Policy regarding the use of the fixed-satellite... Allocation, Assignment, and Use of Radio Frequencies § 2.108 Policy regarding the use of the fixed-satellite allocations in the 3.6-3.7, 4.5-4.8, and 5.85-5.925 GHz bands. The use of the fixed-satellite allocations...

  2. 47 CFR 2.108 - Policy regarding the use of the fixed-satellite allocations in the 3.6-3.7, 4.5-4.8, and 5.85-5...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 1 2014-10-01 2014-10-01 false Policy regarding the use of the fixed-satellite... Allocation, Assignment, and Use of Radio Frequencies § 2.108 Policy regarding the use of the fixed-satellite allocations in the 3.6-3.7, 4.5-4.8, and 5.85-5.925 GHz bands. The use of the fixed-satellite allocations...

  3. 47 CFR 2.108 - Policy regarding the use of the fixed-satellite allocations in the 3.6-3.7, 4.5-4.8, and 5.85-5...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Policy regarding the use of the fixed-satellite... Allocation, Assignment, and Use of Radio Frequencies § 2.108 Policy regarding the use of the fixed-satellite allocations in the 3.6-3.7, 4.5-4.8, and 5.85-5.925 GHz bands. The use of the fixed-satellite allocations...

  4. Structure-based drug design and potent anti-cancer activity of tricyclic 5:7:5-fused diimidazo[4,5-d:4′,5′-f][1,3]diazepines

    PubMed Central

    Kondaskar, Atul; Kondaskar, Shilpi; Fishbein, James C.; Carter-Cooper, Brandon A.; Lapidus, Rena G.; Sadowska, Mariola; Edelman, Martin J.

    2012-01-01

    Judicial structural modifications of 5:7-fused ring-expanded nucleosides (RENs), based on molecular modeling studies with one of its known targets, human RNA helicase (hDDX3), led to the lead, novel, 5:7-5-fused tricyclic heterocycle (1). The latter exhibited promising broad-spectrum in vitro anticancer activity against a number of cancer cell lines screened. This paper describes our systematic, albeit limited, structure-activity relationship (SAR) studies on this lead compound, which produced a number of analogs with broad-spectrum in vitro anticancer activities against lung, breast, prostate, and ovarian cancer cell lines, in particular compounds 15i, 15j, 15m and 15n which showed IC50 values in submicromolar to micromolar range, and are worthy of further explorations. The SAR data also enabled us to propose a tentative SAR model for future SAR efforts for ultimate realization of optimally active and minimally toxic anticancer compounds based on the diimidazo[4,5-d:4′,5′-f][1,3]diazepine structural skeleton of the lead compound 1. PMID:23290252

  5. Synthesis of 5-chloroformycin A, 5-chloro-2'-deoxyformycin A and certain related 5,7-disubstituted 3-beta-D-ribofuranosylpyrazolo[4,3-d] pyrimidines from formycin A.

    PubMed Central

    Upadhya, K G; Sanghvi, Y S; Robins, R K; Revankar, G R; Ugarkar, B G

    1986-01-01

    A facile synthesis of 7-amino-5-chloro-3-beta-D-ribofuranosylpyrazolo [4,3-d]pyrimidine (5-chloroformycin A, 6), 7-amino-5-chloro-3-(2-deoxy-beta-D-erythro-pentofuranosyl) pyrazolo [4,3-d]-pyrimidine (5-chloro-2'-deoxyformycin A, 13) and certain related 5,7-disubstituted pyrazolo[4,3-d]pyrimidine ribonucleosides is described starting with formycin A. Thiation of tri-O-acetyloxoformycin B (4b) with phosphorus pentasulfide, followed 3-beta-D-ribofuranosyl-7-thioxopyrazolo[4,3-d] pyrimidin-5(1H,4H,6H)-one (3b) in excellent yield. Chlorination of 4b with either phosphorus oxychloride or phenyl phosphonicdichloride furnished the key intermediate 5,7-dichloro-3-(2,3, 5-tri-O-acetyl-beta-D-ribofuranosyl)pyrazolo[4,3-d]pyrimidine (5a), which on deacetylation afforded 5,7-dichloro-3-beta-D-ribofuranosylpyrazolo [4,3-d]pyrimidine (5b). Ammonolysis of 5a with liquid ammonia gave 6, whereas with MeOH/NH3, a mixture of 6 and 7-methoxy-5-chloro-3-beta-D-ribofuranosylpyrazolo[4,3-d]pyrimidine (7) was obtained. Reaction of 6 with lithium azide and subsequent hydrogenation afforded 5-aminoformycin A (10). Treatment of 5a with thiourea gave 5-chloro-3-(2,3,5-tri-O-acetyl-beta-D-ribofuranosyl) pyrazolo[4,3-d]pyrimidine-7(1H,6H)-thione (8a), which on further reaction with sodium hydrosulfide furnished 3-beta-D-ribofuranosylpyrazolo [4,3-d]pyrimidine-5,7(1H,4H,6H)-dithione (11). The four-step deoxygenation procedure using phenoxythiocarbonylation of the 2'-hydroxy group of the 3', 5'-protected 6 gave 5-chloro-2'-deoxyformycin A (13). PMID:3951995

  6. Synthesis and properties of new derivatives of ethyl 7-methyl-2,4-dioxo-1,2,3,4-tetrahydropyrido [2,3-d]pyrimidine-5-carboxylate.

    PubMed

    Sladowska, H; Bartoszko-Malik, A; Zawisza, T

    1990-01-01

    Condensation of diethyl 2-amino-6-methylpyridine-3,4-dicarboxylate with phenyl or cyclohexyl isocyanates gave the corresponding derivatives of ethyl 7-methyl-2,4-dioxo-1,2,3,4-tetrahydropyrido [2,3-d]pyrimidine- 5-carboxylate[(V), (VI)]. Alkylation of (V) and (VI) afforded the corresponding N-1 substituted derivatives (XI-XIX). PMID:2337441

  7. Structural, spectroscopic and theoretical studies of the 2:2 complex of 5,5'-dibromo-2,2'-biphenol with 1,5,7-triazabicyclo[4.4.0]dec-5-ene

    NASA Astrophysics Data System (ADS)

    Wojciechowski, G.; Brzezinski, B.; Naumov, P.; Chantrapromma, S.; Ibrahim, A. R.; Fun, H.-K.; Ng, S. W.

    2001-12-01

    In the crystal of the 2:2 complex of 1,5,7-triazabicyclo[4.4.0]dec-5-ene with 5,5'-dibromo-2,2'-biphenol a cooperative inter-intra-molecular hydrogen-bonded system is formed. The intermolecular hydrogen bonds are relatively long [2.708(6) and 2.895(7)] and do not show large proton polarizability. The intramolecular OHO - hydrogen bond is relatively short [2.489(6)]. The structure of the complex is very well reflected in its FT-IR spectrum in the solid. On the basis of the FT-IR and 1H NMR studies of the complex in chloroform and in acetonitrile a structure different than in the solid is proposed. DFT structures of both isolated ions in their ground electronic states at the B3LYP/6-31++G(d,p) level are employed to follow the effects of the hydrogen bonding on the intrinsic ionic structures.

  8. (Eco)toxicological effects of 2,4,7,9-tetramethyl-5-decyne-4,7-diol (TMDD) in zebrafish (Danio rerio) and permanent fish cell cultures.

    PubMed

    Vincze, Krisztina; Gehring, Martin; Braunbeck, Thomas

    2014-01-01

    2,4,7,9-tetramethyl-5-decyne-4,7-diol (TMDD) is a high-production volume chemical used in paper, ink, pesticide, and adhesive industries as a wetting and anti-foaming agent. The physicochemical properties and slow biodegradation rate of TMDD indicate a low bioaccumulation potential but a high prevalence in the environment. As a consequence, TMDD has been detected in several European rivers in the nanogram per liter and lower microgram per liter range; however, its environmental risk to aquatic organisms is considered low. Recent studies almost exclusively focused on acute effects by TMDD, little is known about cytotoxic and genotoxic effects, reproduction and developmental toxicity, endocrine disruption, and any kind of long-term toxicity and carcinogenicity so far. The present study aims to provide more specific baseline information on the ecotoxicological effects of TMDD in fish. For this end, cyto- and genotoxicity assays were carried out in vitro with the permanent fish cell line RTL-W1; in addition, in vivo studies were conducted with the early life stages of zebrafish (Danio rerio) in order to fill the data gaps in developmental toxicity and endocrine disruption. TMDD showed a cytotoxic and slight genotoxic potential in fish cell lines; moreover, various sublethal and lethal effects could be detected in developing zebrafish embryos. There was no evidence of endocrine-disrupting effects by TMDD; however, mortality following prolonged exposure to TMDD during fish sexual development test was clearly higher than mortality in the fish embryo test after 96-h exposure. Our results thus confirmed previous findings of laboratory screening tests, suggesting short-term toxic effects of TMDD in the intermediate, and long-term effects in the lower milligram per liter range. PMID:24687796

  9. Isolation and Characterization of Flavanone Glycoside 4I,5, 7-Trihydroxy Flavanone Rhamnoglucose from Garcinia kola Seed

    NASA Astrophysics Data System (ADS)

    Okwu, D. E.; Morah, F. N. I.

    The ethanolic extract of Garcinia kola, Heckel (Guttiferae), which had previously been shown to have biological activity were studied. Preliminary phytochemical screening of the plants showed the presence of flavonoids, phenolic compounds, tannins and saponins. The ethanolic extract of Garcinia kola seeds resulted in the isolation and characterization of flavanone glycoside 4I, 5, 7-trihydroxyflavonone rhamnoglucose (that is naringin-7-rharmnoglucoseside) from its spectral data. IHNMR spin system analysis and acid hydrolysis were performed to characterize the higher order rhamnoglucosyl moiety comprising glucose and rhamnose linked to carbon 7 of the flavanone ring system of the isolate. It is concluded that 4I, 5, 7-trihydroxyflavanone rhamnoglucose may be a contributor to the antioxidants, anti-inflammatory, anti-microbial, anti-tumor and anti-hepatotoxic properties exhibited by Garcinia kola seed.

  10. Multinuclear NMR spectroscopy and antitumor activity of novel platinum(II) complexes with 5,7-disubstituted-1,2,4-triazolo[1,5-a]pyrimidines.

    PubMed

    Łakomska, Iwona; Szłyk, Edward; Sitkowski, Jerzy; Kozerski, Lech; Wietrzyk, Joanna; Pełczyńska, Marzena; Nasulewicz, Anna; Opolski, Adam

    2004-01-01

    Novel platinum(II) complexes with 5,7-disubstituted-1,2,4-triazolo[1,5-a]pyrimidines have been synthesized and characterized by infrared and multinuclear magnetic resonance spectroscopic techniques (1H, 13C, 15N, 195Pt). The complexes are of two types: [PtCl2(L)2] and [PtCl2(NH3)(L)], where L=5,7-diphenyl-1,2,4-triazolo[1,5-a]pyrimidine (dptp) and 5,7-ditertbutyl-1,2,4-triazolo[1,5-a]pyrimidine (dbtp). Significant 15N NMR upfield shifts (92-95 ppm) were observed for N(3) atom indicating this nitrogen atom as a coordination site. The molecular structure suggest that Pt(II) ion has the square planar geometry with N(3) bonded 5,7-disubstituted-1,2,4-triazolo[1,5-a]pyrimidines, N-bonded second ligand (NH3 for cis-[PtCl2(NH3)(L)] or, respectively, 5,7-disubstituted-1,2,4-triazolo[1,5-a]pyrimidines for cis-[PtCl2L2]) and two cis chloride anions. The antiproliferative activity in vitro of complexes (1-4) have been tested against the cells of four human cell lines: SW707 rectal adenocarcinoma, A549 non-small cell lung carcinoma, T47D breast cancer and HCV29T bladder cancer. The results indicate a moderate antiproliferative activity of (4) against the cells of rectal, breast and bladder cancer and a marked and selective cytotoxic effect of (1-3) against the cells of all studied human cancer lines. PMID:14659646

  11. Synthesis, in vitro antimycobacterial evaluation and docking studies of some new 5,6,7,8-tetrahydropyrido[4',3':4,5]thieno[2,3-d]pyrimidin-4(3H)-one schiff bases.

    PubMed

    Malothu, Narender; Bhandaru, Jaswanth S; Kulandaivelu, Umasankar; Jojula, Malathi; Adidala, Raghuram Reddy; K R, Umadevi; A V N, Dusthackeer; Kaki, Venkat Rao; Akkinepally, Raghuram R

    2016-02-01

    Development of multidrug resistant (MDR) and extensively drug resistant (XDR) tuberculosis (TB) has been considered as major health burden, globally. In order to develop novel, potential molecules against drug resistant TB, twenty two (22) new 3-substituted-7-benzyl-5,6,7,8-tetrahydropyrido[4',3':4,5]thieno[2,3-d]pyrimidin-4(3H)-one (6a-k) and 3-substituted-7-benzyl-2-methyl-5,6,7,8-tetrahydropyrido[4',3':4,5]thieno[2,3-d]pyrimidin-4(3H)-one (7a-k) derivatives were designed and synthesized by using appropriate synthetic protocols. Pantothenate synthetase (PS) was considered as the target for the molecular docking studies and evaluated the binding pattern at active site, as PS plays a significant role in the biosynthesis of pantothenate in Mycobacterium tuberculosis (MTB). The preliminary in vitro antibacterial screening of test compounds was carried out against two strains of Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli and Klebsiella pneumoniae) bacteria. The antimycobacterial screening was performed against MTB H37Rv and an isoniazid-resistant clinical isolate of MTB. The compounds 6b, 6c, 6d, 6k, 7b, 7c, 7d and 7k exhibited promising antibacterial activity MIC in the range of 15-73 μM against all bacterial strains used and compounds 6d and 7b showed antimycobacterial activity (IC50 <340 μM in LRP assay) and (MIC <9 μM in broth microdilution method). PMID:26755393

  12. (Y0.5In0.5)Ba(Co,Zn)4O7 cathodes with superior high-temperature phase stability for solid oxide fuel cells

    SciTech Connect

    Young Nam, Kim; Kim, Jung-Hyun; Paranthaman, Mariappan Parans; Manthiram, Arumugam; Huq, Ashfia

    2012-01-01

    (Y0.5In0.5)BaCo4-xZnxO7 (1.0 x 2.0) oxides crystallizing in a trigonal P31c structure have been synthesized and explored as cathode materials for solid oxide fuel cells (SOFC). At a given Zn content, the (Y0.5In0.5)BaCo4-xZnxO7 sample with 50 % Y and 50 % In exhibits much improved phase stability at intermediate temperatures (600 - 800 oC) compared to the samples with 100 % Y or In. However, the substitution of Zn for Co in (Y0.5In0.5)Ba(Co4-xZnx)O7 (1.0 x 2.0) decreases the amount of oxygen loss on heating, total electrical conductivity, and cathode performance in SOFC while providing good long-term phase stability at high temperatures. Among the various chemical compositions investigated in the (Y0.5In0.5)Ba(Co4-xZnx)O7 system, the (Y0.5In0.5)BaCo3ZnO7 sample offers a combination of good electrochemical performance and low thermal expansion coefficient (TEC) while maintaining superior phase stability at 600 800 oC for 100 h. Fuel cell performances of the (Y0.5In0.5)Ba(Co3Zn)O7 + Ce0.8Gd0.2O1.9 (GDC) (50 : 50 wt. %) composite cathodes collected with anode-supported single cell reveal a maximum power density value of 521 mW cm-2 at 700 oC.

  13. An Efficient Solution-Phase Synthesis of 4,5,7-Trisubstituted Pyrrolo[3,2-d]pyrimidines

    PubMed Central

    Zhang, Weihe; Liu, Jing; Stashko, Michael A.; Wang, Xiaodong

    2013-01-01

    We have developed an efficient and robust route to synthesize 4,5,7-trisubstituted pyrrolo[3,2-d]pyrimidines as potent kinase inhibitors. This solution-phase synthesis features a SNAr substitution reaction, cross-coupling reaction, one-pot reduction/reductive amination and N-alkylation reaction. These reactions occur rapidly with high yields and have broad substrate scopes. A variety of groups can be selectively introduced into the N5 and C7 positions of 4,5,7-trisubstituted pyrrolopyrimidines at a late stage of the synthesis, thereby providing a highly efficient approach to explore the structure-activity relationships of pyrrolopyrimidine derivatives. Four synthetic analogs have been profiled against a panel of 48 kinases and a new and selective FLT3 inhibitor 9 is identified. PMID:23181516

  14. 5,7-Dimeth­oxy-2-(4-methoxy­phen­yl)-4H-1-benzopyran-4-one methanol solvate monohydrate

    PubMed Central

    Aree, Thammarat; Sabphon, Chalisa; Sawasdee, Pattara

    2009-01-01

    In the title compound (alternatively called 4′,5,7-trimethoxy­flavone methanol solvate hydrate), C18H16O5·CH3OH·H2O, the flavone mol­ecule is almost planar, the inter­planar angle between the planes of the benzopyran-4-one group and the attached benzene ring being 4.69 (9)°. In the crystal, the flavone mol­ecule makes inter­molecular C—H⋯O hydrogen bonds to adjacent inversion-related flavone mol­ecules, generating R 2 2(8) and R 2 2(14) rings and an infinite ribbon. The inversion-related ribbons are stabilized through the inter­stitial water and methanol mol­ecules via inter­molecular O—H⋯O hydrogen bonds, generating R 4 2(8) and R 2 1(6) rings and C 2 2(4) chains, and are further sustained by π–π inter­actions with an inter­planar spacing of 3.365 (2)Å. PMID:21578297

  15. 5,7-dihydroxy-2-(3-hydroxy-4, 5-dimethoxy-phenyl)-chromen-4-one-a flavone from Bruguiera gymnorrhiza displaying anti-inflammatory properties

    PubMed Central

    Barik, Rajib; Sarkar, Ratul; Biswas, Prova; Bera, Rammohan; Sharma, Soma; Nath, Suvadeep; Karmakar, Sanmoy; Sen, Tuhinadri

    2016-01-01

    Objective: Bruguiera gymnorrhiza (BRG) (L.) Lamk (Rhizophoraceae), a mangrove species, is widely distributed in the Pacific region, eastern Africa, Indian subcontinent, and subtropical Australia. The leaves of this plant are traditionally used for treating burns and inflammatory lesions. This study isolates the bioactive compound from the methanol extract of BRG leaves and evaluates the possible mechanisms of anti-inflammatory activity involved. Materials and Methods: Bioassay-guided fractionation of BRG was performed to identify the bioactive fraction (displaying inhibition of cyclooxygenase 2 [COX2] - 5-lipoxygenase (5-LOX) activities and tumor necrosis factor-alpha (TNF-α) production at the tested concentrations of 100 and 10 μg/ml). The fractionation was performed by solvent extraction and preparative high-performance liquid chromatography. The bioactive compound was characterized by ultraviolet–visible, liquid chromatography–mass spectrometry and nuclear magnetic resonance spectroscopy. The antioxidant potential was evaluated by electron spin resonance spectrum of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical at 250 μM. The effect of the compound was also studied on TNF-α converting enzyme and nuclear factor kappa B (NF-κB) activities at the concentrations 100, 10 and 1 μg/ml. Results: Bioassay-guided purification of BRG revealed the presence of a flavone (5,7-dihydroxy-2- [3-hydroxy-4,5-dimethoxy-phenyl]-chromen-4-one) of molecular weight 330Da. It demonstrated more than 80% inhibition against COX2, 5-LOX activities and TNF-α production at 100 μg/ml. It also displayed 40% inhibition against DPPH radical at the tested concentration along with 23.1% inhibition of NF-κB activity at 100 μg/ml. Conclusions: The isolated methoxy-flavone may play a predominant role in the anti-inflammatory properties displayed by BRG leaves. Such activity may involve multiple mechanisms, namely (a) modulation of oxidative stress (b) inhibition of arachidonic acid

  16. Synthesis, spectroscopic characterization, X-ray structure and DFT studies on 4-(2-hydroxyphenyl)-4,5,6,7-tetrahydro-1H-imidazo[4,5-c]pyridin-5-ium chloride hydrate.

    PubMed

    Türkyılmaz, Murat; Özdemir, Namık; Baran, Yakup

    2011-11-01

    The title molecular salt, 4-(2-hydroxyphenyl)-4,5,6,7-tetrahydro-1H-imidazo[4,5-c]pyridin-5-ium chloride hydrate (C(12)H(14)N(3)O(+)·Cl-·H(2)O), was synthesized and characterized by IR-NMR spectroscopy and single-crystal X-ray diffraction. In addition to the molecular geometry from X-ray experiment, the molecular geometry, vibrational frequencies and gauge-independent atomic orbital (GIAO) (1)H and (13)C NMR chemical shift values of the title compound in the ground state have been calculated using the density functional theory (DFT/B3LYP) method with the 6-31++G(d,p) and 6-311++G(d,p) basis sets, and compared with the experimental data. Besides, molecular electrostatic potential (MEP) distribution and non-linear optical properties of the title compound were investigated by theoretical calculations at the B3LYP/6-311++G(d,p) level. PMID:21820352

  17. Synthesis, spectroscopic characterization, X-ray structure and DFT studies on 4-(2-hydroxyphenyl)-4,5,6,7-tetrahydro-1 H-imidazo[4,5- c]pyridin-5-ium chloride hydrate

    NASA Astrophysics Data System (ADS)

    Türkyılmaz, Murat; Özdemir, Namık; Baran, Yakup

    2011-11-01

    The title molecular salt, 4-(2-hydroxyphenyl)-4,5,6,7-tetrahydro-1 H-imidazo[4,5- c]pyridin-5-ium chloride hydrate (C 12H 14N 3O +·Clˉ·H 2O), was synthesized and characterized by IR-NMR spectroscopy and single-crystal X-ray diffraction. In addition to the molecular geometry from X-ray experiment, the molecular geometry, vibrational frequencies and gauge-independent atomic orbital (GIAO) 1H and 13C NMR chemical shift values of the title compound in the ground state have been calculated using the density functional theory (DFT/B3LYP) method with the 6-31++G(d,p) and 6-311++G(d,p) basis sets, and compared with the experimental data. Besides, molecular electrostatic potential (MEP) distribution and non-linear optical properties of the title compound were investigated by theoretical calculations at the B3LYP/6-311++G(d,p) level.

  18. Crystal structure of 5,7-diphenyl-4,7-di­hydro­tetra­zolo[1,5-a]pyrimidine

    PubMed Central

    Price, Ivy K.; Rougeot, Celine; Hein, Jason E.

    2015-01-01

    In the title mol­ecule, C16H13N5, the plane of the tetra­zole ring forms dihedral angles of 16.37 (7) and 76.59 (7)° with the two phenyl rings. The dihedral angle between the phenyl rings is 68.05 (6)°. The pyrimidine ring is in a flattened boat conformation. In the crystal, mol­ecules are linked by pairs of N—H⋯N hydrogen bonds, forming inversion dimers. PMID:25844243

  19. Inhibition of thalamic excitability by 4,5,6,7-tetrahydroisoxazolo[4,5-c]pyridine-3-ol: a selective role for delta-GABA(A) receptors.

    PubMed

    Herd, Murray B; Foister, Nicola; Chandra, Dev; Peden, Dianne R; Homanics, Gregg E; Brown, Verity J; Balfour, David J K; Lambert, Jeremy J; Belelli, Delia

    2009-03-01

    The sedative and hypnotic agent 4,5,6,7-tetrahydroisoxazolo[4,5-c]pyridine-3-ol (THIP) is a GABA(A) receptor (GABA(A)R) agonist that preferentially activates delta-subunit-containing GABA(A)Rs (delta-GABA(A)Rs). To clarify the role of delta-GABA(A)Rs in mediating the sedative actions of THIP, we utilized mice lacking the alpha(1)- or delta-subunit in a combined electrophysiological and behavioural analysis. Whole-cell patch-clamp recordings were obtained from ventrobasal thalamic nucleus (VB) neurones at a holding potential of -60 mV. Application of bicuculline to wild-type (WT) VB neurones revealed a GABA(A)R-mediated tonic current of 92 +/- 19 pA, which was greatly reduced (13 +/- 5 pA) for VB neurones of delta(0/0) mice. Deletion of the delta- but not the alpha(1)-subunit dramatically reduced the THIP (1 mum)-induced inward current in these neurones (WT, -309 +/- 23 pA; delta(0/0), -18 +/- 3 pA; alpha(1) (0/0), -377 +/- 45 pA). Furthermore, THIP selectively decreased the excitability of WT and alpha(1) (0/0) but not delta(0/0) VB neurones. THIP did not affect the properties of miniature inhibitory post-synaptic currents in any of the genotypes. No differences in rotarod performance and locomotor activity were observed across the three genotypes. In WT mice, performance of these behaviours was impaired by THIP in a dose-dependent manner. The effect of THIP on rotarod performance was blunted for delta(0/0) but not alpha(1) (0/0) mice. We previously reported that deletion of the alpha(1)-subunit abolished synaptic GABA(A) responses of VB neurones. Therefore, collectively, these findings suggest that extrasynaptic delta-GABA(A)Rs vs. synaptic alpha(1)-subunit-containing GABA(A)Rs of thalamocortical neurones represent an important molecular target underpinning the sedative actions of THIP. PMID:19302153

  20. Synthesis and antiviral activity of certain 4- and 4,5-disubstituted 7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3-d]pyrimidines.

    PubMed

    Pudlo, J S; Saxena, N K; Nassiri, M R; Turk, S R; Drach, J C; Townsend, L B

    1988-11-01

    In vitro evaluation of a series of previously prepared tubercidin analogues revealed that certain 5-halogen-substituted analogues were active against human cytomegalovirus (HCMV) at concentrations lower than those that produced comparable cytotoxicity in uninfected cells. In contrast, tubercidin was cytotoxic at all antiviral concentrations. Even though the antiviral selectivity of the 5-substituted compounds was slight, this observation led us to prepare a series of acyclic analogues. Treatment of the sodium salt of 4-chloropyrrolo[2,3-d]pyrimidine (2) with (2-acetoxyethoxy)methyl bromide (2a) provided the acyclic nucleoside 4-chloro-7-[(2-acetoxyethoxy)methyl]pyrrolo[2,3-d]pyrimidine (3). A nucleophilic displacement of the 4-chloro group with methoxide, methylamine, and dimethylamine yielded the corresponding 4-substituted compounds 4, 5, and 6, respectively, in good yield. Electrophilic substitution (chlorination, bromination, and iodination) was effected at the C-5 position of compound 3 with N-chlorosuccinimide, N-bromosuccinimide, and iodine monochloride, respectively, in methylene chloride. Removal of the acetyl group from these intermediates (7a-9a) with methanolic ammonia at room temperature afforded the 5-chloro (7b), 5-bromo (8b), and 5-iodo (9b) derivatives of 4-chloro-7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3-d]pyrimidine. Treatment of compounds 7b-9b with methanolic ammonia at an elevated temperature produced the corresponding 5-halotubercidin analogues 10, 11, and 12, respectively. An alternate procedure for the preparation of these 4,5-disubstituted 7-[(2-hydroxyethoxy)methyl]pyrrolo[2,3-d]pyrimidines involved an electrophilic substitution prior to the condensation of the heterocycle with 2a. Treatment of 2 with N-chlorosuccinimide and N-bromosuccinimide gave compounds 13a and 13b, respectively. The condensation of 13a and 13b with 2a and subsequent treatment with methylamine and ethylamine furnished the corresponding 5-halo-4-substituted-pyrrolo[2,3-d

  1. Thermal Infrared Radiometric Calibration of the Entire Landsat 4, 5, and 7 Archive (1982-2010)

    NASA Technical Reports Server (NTRS)

    Schott, John R.; Hook, Simon J.; Barsi, Julia A.; Markham, Brian L.; Miller, Jonathan; Padula, Francis P.; Raqueno, Nina G.

    2012-01-01

    Landsat's continuing record of the thermal state of the earth's surface represents the only long term (1982 to the present) global record with spatial scales appropriate for human scale studies (i.e., tens of meters). Temperature drives many of the physical and biological processes that impact the global and local environment. As our knowledge of, and interest in, the role of temperature on these processes have grown, the value of Landsat data to monitor trends and process has also grown. The value of the Landsat thermal data archive will continue to grow as we develop more effective ways to study the long term processes and trends affecting the planet. However, in order to take proper advantage of the thermal data, we need to be able to convert the data to surface temperatures. A critical step in this process is to have the entire archive completely and consistently calibrated into absolute radiance so that it can be atmospherically compensated to surface leaving radiance and then to surface radiometric temperature. This paper addresses the methods and procedures that have been used to perform the radiometric calibration of the earliest sizable thermal data set in the archive (Landsat 4 data). The completion of this effort along with the updated calibration of the earlier (1985 1999) Landsat 5 data, also reported here, concludes a comprehensive calibration of the Landsat thermal archive of data from 1982 to the present

  2. Synthesis, characterization and photophysical properties of novel 5,7-disubstituted-1,4-diazepine-2,3-dicarbonitriles

    NASA Astrophysics Data System (ADS)

    Wieczorek, Ewelina; Gierszewski, Mateusz; Popenda, Lukasz; Tykarska, Ewa; Gdaniec, Maria; Jurga, Stefan; Sikorski, Marek; Mielcarek, Jadwiga; Piskorz, Jaroslaw; Goslinski, Tomasz

    2016-04-01

    Three 5,7-disubstituted-1,4-diazepine-2,3-dicarbonitriles with bulky 2-(3,5-dibromophenyl)ethenyl, 2-(4-tert-butylphenyl)ethenyl and 2-(3,5-dibenzyloxyphenyl)ethenyl substituents were synthesized and characterized using UV-Vis, MS ES, elemental analysis and NMR spectroscopy. NMR data indicated that diazepine rings of all obtained compounds adopted 6H-tautomeric form. In addition, trans-isomerism within styryl substituents was observed. Experimental data for diazepine derivative containing 2-(4-tert-butylphenyl)ethenyl substituents were verified by X-ray crystallography. The obtained compounds were subjected to photophysical studies. In the UV-Vis absorption spectra two characteristic bands were found. In the solvatochromic study, the first band maxima were located in the range of 384-418 nm, whereas second band maxima in the range of 313-345 nm. Fluorescence intensity of novel diazepine derivatives was rather low in all solvents used with the values of fluorescence quantum yield VF = 10-4 for 2-(3,5-dibromophenyl)ethenyl, and 10-5 for 2-(4-tert-butylphenyl)ethenyl and 3,5-(dibenzyloxyphenyl)ethenyl 1,4-diazepine-2,3-dicarbonitriles.

  3. Solid-State Synthesis and Structure of the Enigmatic Ammonium Octaborate: (NH4)2[B7O9(OH)5]·3/4B(OH)3·5/4H2O.

    PubMed

    Neiner, Doinita; Sevryugina, Yulia V; Schubert, David M

    2016-09-01

    The compound known since the 19th century as ammonium octaborate was structurally characterized revealing the ammonium salt of the ribbon isomer of the heptaborate anion, [B7O9(OH)5](2-), with boric acid and water molecules. Of composition (NH4)2B7.75O12.63·4.88H2O, it approximates the classical ammonium octaborate composition (NH4)2B8O13·6H2O and has the structural formula {(NH4)2[B7O9(OH)5]}4·3B(OH)3·5H2O. It spontaneously forms at room temperature in solid-state mixtures of ammonium tetraborate and ammonium pentaborate. It crystallizes in the monoclinic space group P21/c with a = 11.4137(2) Å, b = 11.8877(2) Å, c = 23.4459(3) Å, β = 90.092(1)°, V = 3181.19(8) Å(3), and Z = 2 and contains well-ordered ammonium cations and [B7O9(OH)5](2-) anions and disordered B(OH)3 and H2O molecules linked by extensive H bonding. Expeditious solid-state formation of the heptaborate anion under ambient conditions has important implications for development of practical syntheses of industrially useful borates. PMID:27513178

  4. Novel methyl substituted 1-(5,6-dihydro-[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl)methanones are P2X7 antagonists.

    PubMed

    Rudolph, Dale A; Alcazar, Jesus; Ameriks, Michael K; Anton, Ana Belen; Ao, Hong; Bonaventure, Pascal; Carruthers, Nicholas I; Chrovian, Christa C; De Angelis, Meri; Lord, Brian; Rech, Jason C; Wang, Qi; Bhattacharya, Anindya; Andres, Jose Ignacio; Letavic, Michael A

    2015-08-15

    The optimization efforts that led to a novel series of methyl substituted 1-(5,6-dihydro-[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl)methanones that are potent rat and human P2X7 antagonists are described. These efforts resulted in the discovery of compounds with good drug-like properties that are capable of high P2X7 receptor occupancy in rat following oral administration, including compounds 7n (P2X7 IC50 = 7.7 nM) and 7u (P2X7 IC50 =7 .7 nM). These compounds are expected to be useful tools for characterizing the effects of P2X7 antagonism in models of depression and epilepsy, and several of the compounds prepared are candidates for effective P2X7 PET tracers. PMID:26099534

  5. Extensive theoretical studies on two new members of the FOX-7 family: 5-(dinitromethylene)-1,4-dinitramino-tetrazole and 1,1'-dinitro-4,4'-diamino-5,5'-bitetrazole as energetic compounds.

    PubMed

    He, Piao; Zhang, Jian-Guo; Wang, Kun; Yin, Xin; Jin, Xin; Zhang, Tong-Lai

    2015-02-28

    Two novel compounds 5-(dinitromethylene)-1,4-dinitramino-tetrazole (DNAT) and 1,1'-dinitro-4,4'-diamino-5,5'-bitetrazole (DNABT) were suggested to be potential candidates of high energy density materials (HEDMs). The optimized geometry, NBO charges and electronic density, HOMO-LUMO, electrostatic potential on the surface of molecules, the IR spectrum and thermochemical parameters were calculated for inspecting the electronic structure properties at B3LYP/6-311++G** level of theory. Meanwhile, the solid states of DNAT and DNABT were studied using the crystal packing models by the plane-wave periodic local-density approximation density functional theory. Four stable polymorphous cells have been found including P212121, P21/c, P1̄ and Pbca, assigned to the orthorhombic, monoclinic and triclinic lattice systems. In addition, properties such as density, enthalpy of formation and detonation performance have also been predicted. As a result, the detonation velocity and pressure of two compounds are found to be very remarkable (DNAT: D = 9.17 km s(-1), P = 39.23 GPa; DNABT: D = 9.53 km s(-1), P = 40.92 GPa). Considering the tetrazole rings with energetic groups and the insensitive fragment of FOX-7, high positive heat of formation (583.50 kJ mol(-1) and 1081.39 kJ mol(-1)) and eminent performance render DNAT and DNABT to be very promising powerful energetically insensitive compounds. This work provides theoretical support for further experimental synthesis. PMID:25631492

  6. The thermodynamic properties of 4,5,9,10-tetrahydropyrene and 1,2,3,6,7,8-hexahydropyrene

    SciTech Connect

    Chirico, R.D.; Knipmeyer, S.E.; Nguyen, A.; Smith, N.K.; Steele, W.V.

    1992-12-01

    Measurements leading to the calculation of the ideal-gas thermodynamic properties are reported for 4,5,9,10-tetrahydropyrene and 1,2,3,6,7,8-hexahydropyrene. Experimental methods included combustion calorimetry, adiabatic heat-capacity calorimetry, vibrating-tube densitometry, comparative ebulliometry, inclined-piston gauge manometry, and differential-scanning calorimetry (d.s.c.). Critical properties were estimated for both materials based on the measurement results. Entropies, enthalpies, and Gibbs energies of formation were derived for the ideal gases for selected temperatures between 380 K and 700 K. The property-measurement results reported here for 4,5,9,10-tetrahydropyrene and 1,2,3,6,7,8-hexahydropyrene are the first for these important intermediates in the pyrene/H{sub 2} hydrogenation reaction network.

  7. New 2-phenyl-4,5,6,7-tetrahydro-2H-indazole derivatives as paddy field herbicides.

    PubMed

    Hwang, In Taek; Kim, Hyoung Rae; Jeon, Dong Ju; Hong, Kyung Sik; Song, Jong Hwan; Chung, Chang Kook; Cho, Kwang Yun

    2005-05-01

    A series of 3-chloro-2-(4-chloro-2-fluorophenyl)-4,5,6,7-tetrahydro-2H-indazole derivatives containing various substituted isoxazolinylmethoxy groups at the 5-position of the benzene ring were synthesized and their herbicidal activities assessed under greenhouse and flooded paddy conditions. Among them, compounds having a phenyl or cyano substituent at the 3-position of the 5-methyl-isoxazolin-5-yl structure demonstrated good rice selectivity and potent herbicidal activity against annual weeds at 16-63 g AI ha(-1) under greenhouse conditions. Field trials indicated that these two compounds controlled a wide range of annual weeds rapidly with a good tolerance on transplanted rice seedlings by pre-emergence application. They showed a low mammalian and environmental toxicity in various toxicological tests. PMID:15627239

  8. Cytotoxic, Antiproliferative and Pro-Apoptotic Effects of 5-Hydroxyl-6,7,3′,4′,5′-Pentamethoxyflavone Isolated from Lantana ukambensis

    PubMed Central

    Sawadogo, Wamtinga Richard; Cerella, Claudia; Al-Mourabit, Ali; Moriou, Céline; Teiten, Marie-Hélène; Guissou, Innocent Pierre; Dicato, Mario; Diederich, Marc

    2015-01-01

    Lantana ukambensis (Vatke) Verdc. is an African food and medicinal plant. Its red fruits are eaten and highly appreciated by the rural population. This plant was extensively used in African folk medicinal traditions to treat chronic wounds but also as anti-leishmanial or cytotoxic remedies, especially in Burkina Faso, Tanzania, Kenya, or Ethiopia. This study investigates the in vitro bioactivity of polymethoxyflavones extracted from a L. ukambensis as anti-proliferative and pro-apoptotic agents. We isolated two known polymethoxyflavones, 5,6,7,3′,4′,5′-hexamethoxyflavone (1) and 5-hydroxy-6,7,3′,4′,5′-pentamethoxyflavone (2) from the whole plant of L. ukambensis. Their chemical structures were determined by spectroscopic analysis and comparison with published data. These molecules were tested for the anti-proliferative, cytotoxic and pro-apoptotic effects on human cancer cells. Among them, 5-hydroxy-6,7,3′,4′,5′-pentamethoxyflavone (2) was selectively cytotoxic against monocytic lymphoma (U937), acute T cell leukemia (Jurkat), and chronic myelogenous leukemia (K562) cell lines, but not against peripheral blood mononuclear cells (PBMCs) from healthy donors, at all tested concentrations. Moreover, this compound exhibited significant anti-proliferative and pro-apoptotic effects against U937 acute myelogenous leukemia cells. This study highlights the anti-proliferative and pro-apoptotic effects of 5-hydroxy-6,7,3′,4′,5′-pentamethoxyflavone (2) and provides a scientific basis of traditional use of L. ukambensis. PMID:26690473

  9. Synthesis, structure-activity relationships and biological evaluation of 4,5,6,7-tetrahydropyrazolopyrazines as metabotropic glutamate receptor 5 negative allosteric modulators.

    PubMed

    Hirose, Wataru; Kato, Yoshihiro; Yamamoto, Takayoshi; Kassai, Momoe; Takata, Makoto; Hayashi, Shun; Arai, Yukiyo; Imai, Satoki; Yoshida, Kohzo

    2016-08-15

    The design, synthesis and SAR studies of novel 4,5,6,7-tetrahydropyrazolopyrazines as metabotropic glutamate receptor 5 (mGluR5) negative allosteric modulators (NAMs) are presented in this letter. Starting from a HTS hit compound (1, IC50=477nM), optimization of various groups led to the synthesis of a potent mGluR5 NAM (32, IC50=75nM) with excellent rat PK profile and good brain penetration. This compound produced oral antidepressant-like effect in a mouse tale suspension model (MED: 30mg/kg). PMID:27432763

  10. 40 CFR 721.8485 - 2-Propenoic acid, 2-methyl-, (octahydro-4,7-methano-1H- indene-5-diyl)bis(methylene) ester.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...-, (octahydro-4,7-methano-1H- indene-5-diyl)bis(methylene) ester. 721.8485 Section 721.8485 Protection of...-methyl-, (octahydro-4,7-methano-1H- indene-5-diyl)bis(methylene) ester. (a) Chemical substance and...-, (octahydro-4,7-methano- 1H- indene-5-diyl)bis(methylene) ester (PMN P-99-1075; CAS No. 43048-08-4) is...

  11. 40 CFR 721.8485 - 2-Propenoic acid, 2-methyl-, (octahydro-4,7-methano-1H- indene-5-diyl)bis(methylene) ester.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...-, (octahydro-4,7-methano-1H- indene-5-diyl)bis(methylene) ester. 721.8485 Section 721.8485 Protection of...-methyl-, (octahydro-4,7-methano-1H- indene-5-diyl)bis(methylene) ester. (a) Chemical substance and...-, (octahydro-4,7-methano- 1H- indene-5-diyl)bis(methylene) ester (PMN P-99-1075; CAS No. 43048-08-4) is...

  12. Interconfigurational 5d → 4f luminescence of Ce3+ and Pr3+ in Ca9Lu(PO4)7.

    PubMed

    Trevisani, M; Ivanovskikh, K V; Piccinelli, F; Speghini, A; Bettinelli, M

    2012-09-26

    Ca(9)Lu(PO(4))(7):Ce (3+) and Ca (9)Lu (PO (4))(7):Pr (3+) polycrystalline materials were synthesized by solid state reaction at high temperature. The materials were characterized by powder x-ray diffraction (XRPD). The luminescence spectroscopy and the excited state dynamics of these compounds were investigated upon excitation with UV/VUV synchrotron radiation. Both materials showed efficient and fast 5d-4f emission upon direct VUV excitation into the 5d levels but only Ca(9)Lu(PO(4))(7):Ce (3+) revealed luminescence upon excitation across the bandgap. The decay kinetics of the 5d-4f emission upon VUV intra-center excitation is characterized by a decay time of 29 ns for Ce (3+) and 17 ns for Pr (3+) with no significant build-up after the excitation pulse. For the both compounds, no significant temperature dependence of the 5d-4f emission lifetime was observed within the range 8-300 K. PMID:22944734

  13. Synthesis and Src kinase inhibitory activity of a series of 4-[(2,4-dichloro-5-methoxyphenyl)amino]-7-furyl-3-quinolinecarbonitriles.

    PubMed

    Boschelli, Diane H; Wu, Biqi; Ye, Fei; Wang, Yan; Golas, Jennifer M; Lucas, Judy; Boschelli, Frank

    2006-12-28

    Compound 1 (SKI-606, bosutinib), a 7-alkoxy-4-[(2,4-dichloro-5-methoxyphenyl)amino]-3-quinolinecarbonitrile, is a potent inhibitor of Src kinase activity. We previously reported that analogs of 1 with thiophene groups at C-7 retained the Src activity of the parent compound. The corresponding C-7 furan analogs were prepared and it was found that the 3,5-substituted furan analog had increased activity compared to that of the 2,5-substituted furan isomer. Addition of a methoxy group at C-6 decreased the Src inhibitory activity of the C-7 2,5-substituted furan analog but increased the activity of the C-7 3,5-substituted furan isomer. This compound, 10, was a more potent Src inhibitor than 1 in both enzymatic and cell-based assays. The kinase selectivity profile of 10 was similar to that of 1, with 10 also inhibiting the activity of Abl and Lck. When tested in a solid tumor xenograft model, 10 had comparable oral activity to that of 1. PMID:17181170

  14. 2,2,4-Trimethyl-7-nitro-2,3-dihydro-1H-1,5-benzodiazepin-5-ium perchlorate

    PubMed Central

    Mehdi, Sayed Hasan; Sulaiman, Othman; Ghalib, Raza Murad; Yeap, Chin Sing; Fun, Hoong-Kun

    2010-01-01

    In the title mol­ecular salt, C12H16N3O2 +·ClO4 −, the nitro group is close to being coplanar with the benzene ring [dihedral angle = 8.1 (3)°]. The seven-membered ring has a maximum deviation of 0.502 (3) Å at the C atom between the dimethyl- and methyl-substituted C atoms. In the crystal, the components are linked into infinite sheets lying parallel to the bc plane by N—H⋯O and C—H⋯O hydrogen bonds. A short O⋯N contact of 2.896 (4) Å occurs within the sheets and a short O⋯O contact of 2.608 (4) Å occurs between the sheets. PMID:21588044

  15. Meetings of the American Indian Policy Review Commission (January 6, 7, February 4 and 5, 1977). Volume 5.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. Senate Select Committee on Indian Affairs.

    The meetings of the American Indian Policy Review Commission on January 6, 1977, were concerned with the markup of the first draft of the final report, the status of the Commission extension, training, distribution of task force reports, and transition coordination. The session on February 4 opened with the announcement that the Senate had passed…

  16. Discovery of 7-aryl-substituted (1,5-naphthyridin-4-yl)ureas as aurora kinase inhibitors.

    PubMed

    Defaux, Julien; Antoine, Maud; Le Borgne, Marc; Schuster, Tilmann; Seipelt, Irene; Aicher, Babette; Teifel, Michael; Günther, Eckhard; Gerlach, Matthias; Marchand, Pascal

    2014-01-01

    As part of our research projects to identify new chemical entities of biological interest, we developed a synthetic approach and the biological evaluation of (7-aryl-1,5-naphthyridin-4-yl)ureas as a novel class of Aurora kinase inhibitors for the treatment of malignant diseases based on pathological cell proliferation. 1,5-Naphthyridine derivatives showed excellent inhibitory activities toward Aurora kinases A and B, and the most active compound, 1-cyclopropyl-3-[7-(1-methyl-1H-pyrazol-4-yl)-1,5-naphthyridin-4-yl]urea (49), displayed IC₅₀ values of 13 and 107 nM against Aurora kinases A and B, respectively. In addition, the selectivity toward a panel of seven cancer-related protein kinases was highlighted. In vitro ADME properties were also determined in order to rationalize the difficulties in correlating antiproliferative activity with Aurora kinase inhibition. Finally, the good safety profile of these compounds imparts promising potential for their further development as anticancer agents. PMID:24273104

  17. Development of a new fluorescent probe: 1,3,5,7-tetramethyl-8-(4'-aminophenyl)-4,4-difluoro-4-bora-3a,4a-diaza- s-indacence for the determination of trace nitrite

    NASA Astrophysics Data System (ADS)

    Li, Mengling; Wang, Hong; Zhang, Xian; Zhang, Hua-shan

    2004-03-01

    A new fluorescent probe, 1,3,5,7-tetramethyl-8-(4'-aminophenyl)-4,4-difluoro-4-bora-3a,4a-diaza- s-indacence (TMABODIPY) has been developed for the determination of trace nitrite in terms of the reaction of nitrite with TMABODIPY first in acidic solution and then in alkaline solution to form diazotate, a stable and highly fluorescent reagent. The method offered the advantage of specificity, sensitivity and simplicity. The linear calibration range for nitrite was 8-300 nmol l -1 s with a 3 σ detection limit of 0.65 nmol l -1. The proposed method has been applied to monitor the trace nitrite in drinking water and vegetable without extraction.

  18. Characterisation of Toll-like receptors 4, 5 and 7 and their genetic variation in the grey partridge.

    PubMed

    Vinkler, Michal; Bainová, Hana; Bryjová, Anna; Tomášek, Oldřich; Albrecht, Tomáš; Bryja, Josef

    2015-02-01

    Toll-like receptors (TLRs) are a cornerstone of vertebrate innate immunity. In this study, we identified orthologues of TLR4, TLR5 and TLR7 (representing both bacterial- and viral-sensing TLRs) in the grey partridge (Perdix perdix), a European Galliform game bird species. The phylogeny of all three TLR genes follows the known phylogeny of Galloanserae birds, placing grey partridge TLRs (PePeTLRs) in close proximity to their turkey and pheasant orthologues. The predicted proteins encoded by the PePeTLR genes were 843, 862-863 and 1,047 amino acids long, respectively, and clearly showed all TLR structural features. To verify functionality in these genes we mapped their tissue-expression profiles, revealing generally high PePeTLR4 and PePeTLR5 expression in the thymus and absence of PePeTLR4 and PePeTLR7 expression in the brain. Using 454 next-generation sequencing, we then assessed genetic variation within these genes for a wild grey partridge population in the Czech Republic, EU. We identified 11 nucleotide substitutions in PePeTLR4, eight in PePeTLR5 and six in PePeTLR7, resulting in four, four and three amino acid replacements, respectively. Given their locations and chemical features, most of these non-synonymous substitutions probably have a minor functional impact. As the intraspecific genetic variation of the three TLR genes was low, we assume that either negative selection or a bottleneck may have reduced TLR population variability in this species. PMID:25626717

  19. 2-(Alkylamino)-3-aryl-6,7-dihydrobenzofuran-4(5H)-ones: Improved Synthesis and their Photophysical Properties

    PubMed Central

    Kumar, Manoj; Kumawat, Lokesh Kumar; Gupta, Vinod Kumar; Sharma, Anuj

    2015-01-01

    Furans are an important class of compounds and exhibit a diverse range of activities and properties. As such, improved synthetic access to furans is an important research goal. In the present report, a solvent- and catalyst-free reaction between 5,5-dimethyl-1,3-cyclohexanedione (dimedone), an aryl aldehyde and an isocyanide under microwave irradiation is presented. This method is significantly improved from previously described protocols in terms of applicability of wide ranging aryl aldehydes, better yields, shorter reaction times, facile work up and essentially no need of column chromatography. The photophysical properties of this series of compounds were studied for their possible applicability in the field of metal ion sensors. In solution, two compounds, 2-(cyclohexylamino)-3-(1H-indol-3-yl)-6,6-dimethyl-6,7-dihydrobenzofuran-4(5H)-one (1 i) and 2-(tert-butylamino)-3-(1H-indol-3-yl)-6,6-dimethyl-6,7-dihydrobenzofuran-4(5H)-one (1 j), underwent an observable color change from yellow to colorless in the presence of aluminum(III) ions. Further studies to investigate the UV absorption and luminescence behavior of these compounds revealed their utility as “naked-eye sensors” for aluminum detection. PMID:26491643

  20. 4-Imino-2,7-dimethyl-5,6,7,8-tetra-hydro-4H-1-benzothieno[2,3-d]pyrimidin-3-amine.

    PubMed

    Kalashetti, Mallikarjun B; Fathima, Nikhath; Khan, Ashraf Y; Begum, Noor Shahina; Khazi, I M

    2012-08-01

    In the title compound, C(12)H(16)N(4)S, the fused benzothio-phene and the pyrimidine rings are coplanar [dihedral angle = 1.61 (6)°]. Three C atoms of the cyclohexene ring (at positions 3, 6 and 7) are disordered over two sites with an occupancy ratio of 0.702 (8):0.298 (8). The cyclo-hexene ring in both the major and minor components adopts a half-chair conformation. The crystal structure is stabilized by N-H⋯N and C-H⋯N inter-actions, resulting in the formation of inversion dimers with R(2) (2)(10) and R(2) (2)(12) graph-set motifs. PMID:22904911

  1. The 2.1-, 5.4- and 5.7-kb transcripts of the IDS gene are generated by different polyadenylation signals.

    PubMed

    Cudry, S; Froissart, R; Bouton, O; Maire, I; Bozon, D

    1999-10-01

    Deficiency of the lysosomal enzyme iduronate-2-sulfatase (IDS) is responsible for mucopolysaccharidosis type II (OMIM 309900). The IDS gene (Xq28) has been completely sequenced (accession number L35485). Northern blot analysis of poly(A(+)) RNA from different tissues, hybridized with the total IDS cDNA, has revealed three major species of 2.1, 5.4 and 5.7 kb and one minor of 1.4 kb. The 1.4-kb mRNA has been previously described and we show that the three major IDS mRNA are the result of alternative polyadenylation site selection: a non-canonical ATTAAA signal at genomic position 23631 for the 2.1-kb mRNA, a AATAAA signal at position 27156 for the 5.4-kb mRNA and a AATAAA signal at position 27399 for the 5.7-kb mRNA. The different IDS mRNA encode for the same polypeptide and the most abundant transcripts have a long 3'-untranslated region (3'-UTR). The absence of obvious correlation between transcripts content and size, IDS protein amount and IDS activity in the four human fetal tissues tested suggests that it is IDS protein processing that may be regulated rather than IDS gene transcription. PMID:10500241

  2. Ethyl 2,7,7-trimethyl-4-(1-methyl-1H-indol-3-yl)-5-oxo-1,4,5,6,7,8-hexa-hydro-quinoline-3-carboxyl-ate.

    PubMed

    Oztürk Yildirim, Sema; Butcher, Ray J; Gündüz, Miyase Gözde; El-Khouly, Ahmed; Simşek, Rahime; Safak, Cihat

    2013-01-01

    In the title mol-ecule, C24H28N2O3, the cyclo-hexene ring is in a sofa conformation and the 1,4-dihydro-pyridine ring is in a slight boat conformation. In the indole ring system, the pyrrole and benzene rings form a dihedral angle of 2.63 (7)°. In the crystal, N-H⋯O hydrogen bonds connect the mol-ecules into C(6) chains parallel to the b axis and pairs of weak C-H⋯O hydrogen bonds link inversion-related chains into a ladder motif through R2(2)(18) rings. A weak intra-molecular C-H⋯O hydrogen bond is also observed. PMID:23476426

  3. Platinum(II) complexes with 5,7-disubstituted-1,2,4-triazolo [1,5-a]pyrimidines: Spectroscopical characterization and cytotoxic activity in vitro

    NASA Astrophysics Data System (ADS)

    Łakomska, Iwona; Fandzloch, Marzena; Popławska, Beata; Sitkowski, Jerzy

    2012-06-01

    Complexes of the types: cis-[PtI2(dptp)2] (1), cis-[PtI2(NH3)(dptp)] (2), trans-[PtI2(dptp)(dmso)] (3) and trans-[PtI2(dbtp)(dmso)] (4), where dptp = 5,7-diphenyl-1,2,4-triazolo[1,5-a]pyrimidine (dptp), dbtp = 5,7-ditertbutyl-1,2,4-triazolo[1,5-a]pyrimidine have been synthesized and characterized by infrared and multinuclear magnetic resonance spectroscopic techniques (1H, 13C, 15N, 195Pt). In 195Pt NMR, the cis-diiodo complexes were observed between -2601 ppm and -3261 ppm, while the trans coordination compounds were found at higher field (ca. -4389 ppm). In all cases significant 15N NMR shielding (92-95 ppm) were observed for N(3) atom indicating this nitrogen atom as a coordination site. The cis complexes have been assayed for antitumor activity in vitro against two human cell lines: A549 (non-small cell lung carcinoma) and T47D (breast cancer). The results indicate a moderate antiproliferative activity of (2) against human cancer lines.

  4. Crystal structures of 7-methylguanosine 5'-triphosphate (m(7)GTP)- and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)-bound human full-length eukaryotic initiation factor 4E: biological importance of the C-terminal flexible region.

    PubMed Central

    Tomoo, Koji; Shen, Xu; Okabe, Koumei; Nozoe, Yoshiaki; Fukuhara, Shoichi; Morino, Shigenobu; Ishida, Toshimasa; Taniguchi, Taizo; Hasegawa, Hiroshi; Terashima, Akira; Sasaki, Masahiro; Katsuya, Yoshio; Kitamura, Kunihiro; Miyoshi, Hiroshi; Ishikawa, Masahide; Miura, Kin-ichiro

    2002-01-01

    The crystal structures of the full-length human eukaryotic initiation factor (eIF) 4E complexed with two mRNA cap analogues [7-methylguanosine 5'-triphosphate (m(7)GTP) and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)] were determined at 2.0 A resolution (where 1 A=0.1 nm). The flexibility of the C-terminal loop region of eIF4E complexed with m(7)GTP was significantly reduced when complexed with m(7)GpppA, suggesting the importance of the second nucleotide in the mRNA cap structure for the biological function of eIF4E, especially the fixation and orientation of the C-terminal loop region, including the eIF4E phosphorylation residue. The present results provide the structural basis for the biological function of both N- and C-terminal mobile regions of eIF4E in translation initiation, especially the regulatory function through the switch-on/off of eIF4E-binding protein-eIF4E phosphorylation. PMID:11879179

  5. Stability of ferric complexes with 3-hydroxyflavone (flavonol), 5,7-dihydroxyflavone (chrysin), and 3',4'-dihydroxyflavone.

    PubMed

    Engelmann, Mark D; Hutcheson, Ryan; Cheng, I Francis

    2005-04-20

    The acid dissociation and ferric stability constants for complexation by the flavonoids 3-hydroxyflavone (flavonol), 5,7-dihydroxyflavone (chrysin), and 3',4'-dihydroxyflavone in 50:50 (v/v) ethanol/water are determined by pH potentiometric and spectrophotometric titrations and the linear least-squares curve-fitting program Hyperquad. Over the entire range of pH and reagent concentrations spanning the titration experiments, the stoichiometry for iron-flavonoid complex formation was 1:1 for all three flavonoids examined. The three flavonoids were chosen for their hydroxy substitution pattern, with each possessing one of the three most commonly suggested sites for metal binding by the flavonoids. On the basis of the calculated stability constants, the intraflavonoid-binding site competition is illustrated as a function of pH via speciation curves. The curves indicate that the binding site comprised of the 3',4'-hydroxy substitutions, the catecholic site, is most influential for ferric complexation at the physiological pH of 7.4. The possibility for antioxidant activity by flavonoid chelation of ferric iron in the presence of other competitive physiological complexing agents is demonstrated through additional speciation calculations. PMID:15826045

  6. Flavonoid glucosylation by non-Leloir glycosyltransferases: formation of multiple derivatives of 3,5,7,3',4'-pentahydroxyflavane stereoisomers.

    PubMed

    Overwin, Heike; Wray, Victor; Hofer, Bernd

    2015-11-01

    Flavonoids are known to possess a multitude of biological activities. Therefore, diversification of the core structures is of considerable interest. One of nature's important tailoring reactions in the generation of bioactive compounds is glycosylation, which is able to influence numerous molecular properties. Here, we examined two non-Leloir glycosyltransferases that use sucrose as an inexpensive carbohydrate donor, glycosyltransferase R from Streptococcus oralis (GtfR) and amylosucrase from Neisseria polysaccharea (Ams), for the glucosylation of flavonoids. Flavones generally were poor substrates. Several inhibited Ams. In contrast, flavanes were well accepted by both enzymes. All glucose attachments occurred via α1 linkages. Comparison of the three available stereoisomers of 3,5,7,3',4'-pentahydroxyflavane revealed significant differences in glycoside formation between them as well as between the two enzymes. The latter were shown to possess largely complementary product ranges. Altogether, three of the four hydroxy substituents of the terminal flavonoid rings were glycosylated. Typically, Ams glucosylated the B ring at position 3', whereas GtfR glucosylated this ring at position 4' and/or the A ring at position 7. In several instances, short carbohydrate chains were attached to the aglycones. These contained α 1-4 linkages when formed by Ams, but α 1-3 bonds when generated by GtfR. The results show that both enzymes are useful catalysts for the glucodiversification of flavanes. In total, more than 16 products were formed, of which seven have previously not been described. PMID:26124069

  7. Discovery of 8-Cyclopentyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-7-oxo-7,8-dihydro-pyrido[2,3-d]pyrimidine-6-carbonitrile (7x) as a Potent Inhibitor of Cyclin-Dependent Kinase 4 (CDK4) and AMPK-Related Kinase 5 (ARK5)

    PubMed Central

    2015-01-01

    The success of imatinib, a BCR-ABL inhibitor for the treatment of chronic myelogenous leukemia, has created a great impetus for the development of additional kinase inhibitors as therapeutic agents. However, the complexity of cancer has led to recent interest in polypharmacological approaches for developing multikinase inhibitors with low toxicity profiles. With this goal in mind, we analyzed more than 150 novel cyano pyridopyrimidine compounds and identified structure–activity relationship trends that can be exploited in the design of potent kinase inhibitors. One compound, 8-cyclopentyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-7-oxo-7,8-dihydro-pyrido[2,3-d]pyrimidine-6-carbonitrile (7x), was found to be the most active, inducing apoptosis of tumor cells at a concentration of approximately 30–100 nM. In vitro kinase profiling revealed that 7x is a multikinase inhibitor with potent inhibitory activity against the CDK4/CYCLIN D1 and ARK5 kinases. Here, we report the synthesis, structure–activity relationship, kinase inhibitory profile, in vitro cytotoxicity, and in vivo tumor regression studies by this lead compound. PMID:24417566

  8. Complete Genome Sequence of the Marine Cellulose- and Xylan-Degrading Bacterium Glaciecolasp. Strain 4H-3-7+YE-5

    PubMed Central

    Klippel, Barbara; Lochner, Adriane; Bruce, David C.; Walston Davenport, Karen; Detter, Chris; Goodwin, Lynne A.; Han, James; Han, Shunsheng; Land, Miriam L.; Mikhailova, Natalia; Nolan, Matt; Pennacchio, Len; Pitluck, Sam; Tapia, Roxanne; Woyke, Tanja; Wiebusch, Sigrid; Basner, Alexander; Abe, Fumiyoshi; Horikoshi, Koki; Keller, Martin; Antranikian, Garabed

    2011-01-01

    Glaciecolasp. strain 4H-3-7+YE-5 was isolated from subseafloor sediments at Suruga Bay in Japan and is capable of efficiently hydrolyzing cellulose and xylan. The complete genome sequence of Glaciecolasp. 4H-3-7+YE-5 revealed several genes encoding putatively novel glycoside hydrolases, offering a high potential for plant biomass degradation. PMID:21705587

  9. Yellow-light generation of 5.7 W by intracavity doubling self-Raman laser of YVO(4)/Nd:YVO(4) composite.

    PubMed

    Zhu, Haiyong; Duan, Yanmin; Zhang, Ge; Huang, Chenghui; Wei, Yong; Chen, Weidong; Huang, Yidong; Ye, Ning

    2009-09-15

    A high-power 588 nm light produced by an intracavity frequency-doubling acousto-optic Q-switched self-frequency Raman laser is reported. A 20-mm-long YVO(4)/Nd:YVO(4) composite crystal and a 15-mm-long LiB(3)O(5) (LBO) with noncritical phase-matching (theta=90 degrees , phi=0 degrees ) cut were adopted for efficient self-Raman laser operation and second-harmonic generation, respectively. By the optimizing the focus position of incident pump light and Q-switch repetition rate, yellow light with 5.7 W average power was generated under the pump power of 23.5 W, corresponding to the overall diode-yellow conversion efficiency of 24.2% and slope efficiency of 32%. The pulse width is about 16 ns, and the pulse energy is up to 95 microJ. PMID:19756097

  10. Synthesis and anticancer activities of 5,6,7-trimethoxy-N-phenyl(ethyl)-4-aminoquinazoline derivatives.

    PubMed

    Zhang, Ying; Jin, Linhong; Xiang, Hongmei; Wu, Jian; Wang, Peiyi; Hu, Deyu; Xue, Wei; Yang, Song

    2013-08-01

    A series of 5,6,7-trimethoxy-N-phenyl(ethyl)-4-aminoquinazoline compounds was prepared by microwave irradiation and conventional heating methods. Compounds 6p, 6q, and 6x strongly inhibited extracellular regulated kinase1/2 (ERK1/2) phosphorylation induced by epidermal growth factor (EGF) at 1.28 μM in PC3 cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that all compounds had certain anticancer activities, and the IC₅₀ values of 6x were 6.2 ± 0.9, 3.2 ± 0.1, and 3.1 ± 0.1 μM against PC3, BGC823, and Bcap37 cells, respectively. Acridine orange/ethidium bromide staining, Hoechst 33258 staining, DNA ladder, and flow cytometry analyses revealed that 6x induced cell apoptosis in PC3 cells, with apoptosis ratios of 11.6% at 1 μM and 31.8% at 10 μM after 72 h. PMID:23811258

  11. Crystal structure and absolute configuration of (3aS,4S,5R,7aR)-2,2,7-trimethyl-3a,4,5,7a-tetra­hydro-1,3-benzodioxole-4,5-diol

    PubMed Central

    Macías, Mario A.; Suescun, Leopoldo; Pandolfi, Enrique; Schapiro, Valeria; Tibhe, Gaurao D.; Mombrú, Álvaro W.

    2015-01-01

    The absolute configuration of the title compound, C10H16O4, determined as 3aS,4S,5R,7aR on the basis of the synthetic pathway, was confirmed by X-ray diffraction. The mol­ecule contains a five- and a six-membered ring that adopt twisted and envelope conformations, respectively. The dihedral angle between the mean planes of the rings is 76.80 (11)° as a result of their cis-fusion. In the crystal, mol­ecules are linked by two pairs of O—H⋯O hydrogen bonds, forming chains along [010]. These chains are further connected by weaker C—H⋯O inter­actions along [100], creating (001) sheets that inter­act only by weak van der Waals forces. PMID:26396837

  12. Pressure distribution data from tests of 2.29-meter (7.5-ft.) span EET high-lift research model in Langley 4- by 7-meter tunnel

    NASA Technical Reports Server (NTRS)

    Morgan, H. L., Jr.

    1982-01-01

    A 2.29 m (7.5 ft.) span high-lift research model equipped with full-span leading-edge slat and part-span double-slotted trailing-edge flap was tested in the Langley 4- by 7-Meter Tunnel to determine the low speed performance characteristics of a representative high aspect ratio suprcritical wing. These tests were performed in support of the Energy Efficient Transport (EET) program which is one element of the Aircraft Energy Efficiency (ACEE) project. Static longitudinal forces and moments and chordwise pressure distributions at three spanwise stations were measured for cruise, climb, two take-off flap, and two landing flap wing configurations. The tabulated and plotted pressure distribution data is presented without analysis or discussion.

  13. 40 CFR 721.9577 - Chromate(3-), bis[7-[(aminohydroxyphenyl)azo]-3-[[5-(aminosulfonyl)-2-hydroxyphenyl] azo]-4...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Chromate(3-), bis -3- azo]-4-hydroxy-2... Substances § 721.9577 Chromate(3-), bis -3- azo]-4-hydroxy-2-naphthalene sulfonato (3-)]-,- azo]-4-hydroxy-7... significant new uses subject to reporting. (1) The chemical substance identified as chromate(3-), bis -3-...

  14. 40 CFR 721.9577 - Chromate(3-), bis[7-[(aminohydroxyphenyl)azo]-3-[[5-(aminosulfonyl)-2-hydroxyphenyl] azo]-4...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Chromate(3-), bis -3- azo]-4-hydroxy-2... Substances § 721.9577 Chromate(3-), bis -3- azo]-4-hydroxy-2-naphthalene sulfonato (3-)]-,- azo]-4-hydroxy-7... significant new uses subject to reporting. (1) The chemical substance identified as chromate(3-), bis -3-...

  15. 40 CFR 721.9577 - Chromate(3-), bis[7-[(aminohydroxyphenyl)azo]-3-[[5-(aminosulfonyl)-2-hydroxyphenyl] azo]-4...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Chromate(3-), bis -3- azo]-4-hydroxy-2... Substances § 721.9577 Chromate(3-), bis -3- azo]-4-hydroxy-2-naphthalene sulfonato (3-)]-,- azo]-4-hydroxy-7... significant new uses subject to reporting. (1) The chemical substance identified as chromate(3-), bis -3-...

  16. 40 CFR 721.9577 - Chromate(3-), bis[7-[(aminohydroxyphenyl)azo]-3-[[5-(aminosulfonyl)-2-hydroxyphenyl] azo]-4...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Chromate(3-), bis -3- azo]-4-hydroxy-2... Substances § 721.9577 Chromate(3-), bis -3- azo]-4-hydroxy-2-naphthalene sulfonato (3-)]-,- azo]-4-hydroxy-7... significant new uses subject to reporting. (1) The chemical substance identified as chromate(3-), bis -3-...

  17. 40 CFR 721.9577 - Chromate(3-), bis[7-[(aminohydroxyphenyl)azo]-3-[[5-(aminosulfonyl)-2-hydroxyphenyl] azo]-4...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Chromate(3-), bis -3- azo]-4-hydroxy-2... Substances § 721.9577 Chromate(3-), bis -3- azo]-4-hydroxy-2-naphthalene sulfonato (3-)]-,- azo]-4-hydroxy-7... significant new uses subject to reporting. (1) The chemical substance identified as chromate(3-), bis -3-...

  18. Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b.

    PubMed

    Fornuskova, Daniela; Stiburek, Lukas; Wenchich, Laszlo; Vinsova, Kamila; Hansikova, Hana; Zeman, Jiri

    2010-06-15

    Mammalian CcO (cytochrome c oxidase) is a hetero-oligomeric protein complex composed of 13 structural subunits encoded by both the mitochondrial and nuclear genomes. To study the role of nuclear-encoded CcO subunits in the assembly and function of the human complex, we used stable RNA interference of COX4, COX5A and COX6A1, as well as expression of epitope-tagged Cox6a, Cox7a and Cox7b, in HEK (human embryonic kidney)-293 cells. Knockdown of Cox4, Cox5a and Cox6a resulted in reduced CcO activity, diminished affinity of the residual enzyme for oxygen, decreased holoCcO and CcO dimer levels, increased accumulation of CcO subcomplexes and gave rise to an altered pattern of respiratory supercomplexes. An analysis of the patterns of CcO subcomplexes found in both knockdown and overexpressing cells identified a novel CcO assembly intermediate, identified the entry points of three late-assembled subunits and demonstrated directly the essential character as well as the interdependence of the assembly of Cox4 and Cox5a. The ectopic expression of the heart/muscle-specific isoform of the Cox6 subunit (COX6A2) resulted in restoration of both CcO holoenzyme and activity in COX6A1-knockdown cells. This was in sharp contrast with the unaltered levels of COX6A2 mRNA in these cells, suggesting the existence of a fixed expression programme. The normal amount and function of respiratory complex I in all of our CcO-deficient knockdown cell lines suggest that, unlike non-human CcO-deficient models, even relatively small amounts of CcO can maintain the normal biogenesis of this respiratory complex in cultured human cells. PMID:20307258

  19. Molecular and crystal structures of some novel derivatives of 3-aryl-7-arylidene-3,3a,4,5,6,7-hexahydroindazoles

    SciTech Connect

    Abakumov, V. V.; Shishkina, S. V.; Zubatyuk, R. I.; Gella, I. M.; Pivnenko, N. S.; Kutulya, L. A. Shishkin, O. V.

    2007-03-15

    The stereochemical aspects of the interaction of 2,6-bis(arylidene)-cyclohexanone and 2,6-bis(arylidene)-3-methylcyclohexanone with arylhydrazine (aryl is phenyl or 4-nitrophenyl) and methylhydrazine are investigated using X-ray diffraction analysis. The molecular structure of the 3-aryl-7-arylidene-3,3a,4,5,6,7-hexahydroindazoles synthesized is determined by X-ray diffraction analysis for the first time. It is established that the stereochemistry of the products of the interaction between the cyclohexanone derivatives and arylhydrazines depends on the electronic nature of the substituent in the aryl group. Two regioisomeric products with different positions of the methyl group in the cyclohexane ring with respect to the arylidene fragment are synthesized by the reaction of 2,6-bis(4-methoxybenzylidene)-3-methylcyclohexanone with methylhydrazine. The influence of the substituents at the nitrogen atom of the pyrazoline ring on the intramolecular electronic interactions and the geometry of the heterocycle in the compounds under investigation is discussed.

  20. C,O-dialkylation of Meldrum's acid: synthesis and reactivity of 1,3,7,7-tetramethyl-4H,10H-6,8,9-trioxa-2-thiabenz[f]azulen-5-one.

    PubMed

    Snyder, Chad A; Selegue, John P; Dosunmu, Eniolami; Tice, Nathan C; Parkin, Sean

    2003-09-19

    Reaction of Meldrum's acid with 3,4-bis(chloromethyl)-2,5-dimethylthiophene (1) or 3,4-bis(bromomethyl)-2,5-dimethylthiophene (2) produces the kinetically favored C,O-dialkylation product, 1,3,7,7-tetramethyl-4H,10H-6,8,9-trioxa-2-thiabenz[f]azulen-5-one (4). Recrystallization of 4 from refluxing methanol results in the methanolysis product 5-(4-methoxymethyl-2,5-dimethylthiophen-3-ylmethyl)-2,2-dimethyl[1,3]dioxane-4,6-dione (5). Attempts to isomerize 4 to the thermodynamically favored C,C-dialkylation product, 1,3-dimethyl-5,6-dihydro-4H-cyclopenta[c]thiophene(2-spiro-5)2,2-dimethyl-4,6-dione (8), result in the formation of 1,3-dimethyl-7,8-dihydro-4H-thieno[3,4-c]oxepin-6-one (6). The transformation occurs via a retro-Diels-Alder elimination of acetone followed by hydrolysis and decarboxylation of the resulting ketene. The ketene is trapped by tert-butyl alcohol, furnishing 1,3-dimethyl-6-oxo-7,8-dihydro-4H,6H-thieno[3,4-c]oxepine-7-carboxylic acid tert-butyl ester (7). All compounds are characterized spectroscopically as well as by X-ray crystallography of products 4-7. PMID:12968900

  1. Synthesis and biological activity of novel series of 4-methoxy, and 4,9-dimethoxy-5-substituted furo[2,3-g]-1,2,3-benzoxathiazine-7,7-dioxide derivatives

    PubMed Central

    El-Sawy, Eslam R.; Ebaid, Manal S.; Abo-Salem, Heba M.; El-Hallouty, Salwa; Kassem, Emad M.; Mandour, Adel H.

    2013-01-01

    A novel series of 4-methoxy, and 4,9-dimethoxy-5-substituted furo[2,3-g]-1,2,3-benzoxathiazine-7,7-dioxide derivatives 3a,b, 10a–g and 11a–g were prepared in good yields via the reaction of 4-methoxy (1a) and 4,7-dimethoxy-5-acetyl-6-hydroxybenzofurans (1b) and their α,β-unsaturated keto derivatives 6a–g and 7a–g with chlorosulfonyl isocyanate (CSI). On the other hand, N-chlorosulfonyl carbamate derivatives 4a,b, 12a,b and 13a,b were prepared and allowed to react with piperidine to give the corresponding N-piperidinosulfonyl carbamate derivatives 5a,b, 14a,b and 15a,b, respectively. Sixteen new target compounds 3a,b, 10a–g, and 11a–g were tested for their DPPH radical-scavenging, and in vitro antiproliferative activity against A-549, MCF7 and HCT-116 cancer cell lines. Compounds 10a, 11c, 11e, and 11g showed moderate DPPH radical-scavenging activity compared to ascorbic acid at 100 μg/mL. 4,9-Dimethoxy-5-substituted styrylfuro[3,2-g]-1,2,3-benzoxathiazine-7,7-dioxides 11a, 11b, and 11c were found to be highly active against A-549 and HCT-116 cancer cell lines with IC50 values ranging from 0.02 to 0.08 μmol/mL compared to doxorubicin with IC50 = 0.04 and 0.06 μmol/mL, respectively. PMID:25685501

  2. 5,7-Bis(benz­yloxy)-2-[4-(benz­yloxy)phen­yl]-4H-chromen-4-one

    PubMed Central

    Ren, Xu-Hong; Xie, Wei-Jia; Ma, Chao; Wang, Jing-Jing; Cheng, Mao-Sheng

    2010-01-01

    In the title compound, C36H28O5, the terminal benzene rings are twisted at dihedral angles of 6.75 (12), 70.86 (14) and 82.02 (12)° with the respect to the central plana r[maximum deviation = 0.070 (3) Å] chromen-4-one ring system. In the crystal structure, π–π stacking is observed between parallel benzene rings of adjacent mol­ecules [centroid–centroid distance = 3.7459 (16) Å]. PMID:21580165

  3. A Study on the Base–Catalyzed Reverse Vinylogous Aldol Reaction of (4aβ,5β)-4,4a,5,6,7,8-Hexahydro-5-hydroxy-1,4a-dimethylnaphthalen-2(3H)-one under Robinson Annulation Conditions

    PubMed Central

    Payette, Joshua N.; Honda, Tadashi; Yoshizawa, Hidenori; Favaloro, Frank G.; Gribble, Gordon W.

    2008-01-01

    We have proposed a pathway of the base–catalyzed reverse vinylogous aldol reaction of (−)-(4aβ,5β)-4,4a,5,6,7,8-hexahydro-5-hydroxy-1,4a-dimethylnaphthalen-2(3H)-one ((−)-8) under Robinson annulation conditions. For confirmation, 4-(2,6-dimethyl-3-oxocyclohex-1-enyl)butanal (11) and 4-(2,6-dimethyl-5-oxocyclohex-1-enyl)butanal (12), both of which potentially produce enolate I, were synthesized regioselectively. Unexpectedly, 11 gave a complex mixture including only a trace amount of (±)-8 (less than 5% yield) under these basic conditions. To the contrary, 12 cleanly afforded (±)-8 in 66% yield. This result provides evidence for our proposed mechanism of the above reaction. PMID:16388674

  4. Arangasite, Al2(PO4)(SO4)F · 7.5H2O, a new mineral from the Alyaskitovy deposit, Eastern Yakutia, Russia

    NASA Astrophysics Data System (ADS)

    Gamyanin, G. N.; Zayakina, N. V.; Galenchikova, L. T.

    2014-12-01

    A new hydrous aluminum sulfate-phosphate-fluoride arangasite, Al2(PO4)(SO4)F · 7.5H2O, has been found in cassiterite-silicate-sulfide ore at the Alyaskitovy deposit, Indigirka River basin, eastern Sakha (Yakutia) (64°39' N, 142°70' E). The new mineral was named after its type locality, Arangas Creek. It belongs to the secondary minerals of the oxidation zone and occurs in cavities within quartz-muscovite-tourmaline-sulfide veins and adjacent greisen. Arangasite is associated with other secondary minerals: phosphorscorodite, fluellite, gypsum, colquiriite, strengite, mansfieldite, and sinkankasite. Arangasite forms white compact segregations composed of fine-lamellar aggregates. This paper reports data on its chemical composition, optical, radiographic, thermal, and IR-spectroscopic characteristics.

  5. Discovery of novel 5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyridine derivatives as γ-secretase modulators (Part 2).

    PubMed

    Takai, Takafumi; Koike, Tatsuki; Nakamura, Minoru; Kajita, Yuichi; Yamashita, Toshiro; Taya, Naohiro; Tsukamoto, Tetsuya; Watanabe, Tomomichi; Murakami, Koji; Igari, Tomoko; Kamata, Makoto

    2016-07-15

    γ-Secretase modulators (GSMs), which lower pathogenic amyloid beta (Aβ) without affecting the production of total Aβ or Notch signal, have emerged as a potential therapeutic agent for Alzheimer's disease (AD). A novel series of 5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyridine derivatives was discovered and characterized as GSMs. Optimization of substituents at the 8-position of the core scaffold using ligand-lipophilicity efficiency (LLE) as a drug-likeness guideline led to identification of various types of high-LLE GSMs. Phenoxy compound (R)-17 exhibited especially high LLE as well as potent in vivo Aβ42-lowering effect by single administration. Furthermore, multiple oral administration of (R)-17 significantly reduced soluble and insoluble brain Aβ42, and ameliorated cognitive deficit in novel object recognition test (NORT) using Tg2576 mice as an AD model. PMID:27255179

  6. Cr(III)-HMC (HMC = 5,5,7,12,12,14-Hexamethyl-1,4,8,11-tetraazacyclotetradecane) Alkynyl Complexes: Preparation and Emission Properties.

    PubMed

    Tyler, Sarah F; Judkins, Eileen C; Song, You; Cao, Fan; McMillin, David R; Fanwick, Phillip E; Ren, Tong

    2016-09-01

    Presented here is the chemistry of Cr(III) alkynyl complexes based on the rac-HMC and meso-HMC ligands (HMC = 5,5,7,12,12,14-hexamethyl-1,4,8,11-tetraazacyclotetradecane). Thus far, two pairs of cis/trans-[Cr(rac/meso-HMC)(C2R)2]Cl (R = Ph, C2H/C2SiMe3) complexes have been synthesized from reactions between cis/trans-[Cr(rac/meso-HMC)Cl2]Cl and LiC2R. These complexes were characterized using single crystal X-ray diffraction, UV-vis spectroscopy, FT-IR spectroscopy, and fluorimetry. Single crystal X-ray diffraction studies revealed that these complexes adopt a pseudo-octahedral geometry. The electronic spectra of both the cis- and trans-[Cr(rac/meso-HMC)(C4R')2]Cl (R' = H or SiMe3) complexes exhibit d-d bands with pronounced vibronic progression associated with the asymmetric stretch of the Cr-bound C≡C bonds. All of these complexes are phosphorescent and show structured emissions originating from the ligand field excited states. PMID:27529498

  7. Synthesis, spectral characterization, crystal structure and molecular docking study of 2,7-diaryl-1,4-diazepan-5-ones

    NASA Astrophysics Data System (ADS)

    Sethuvasan, S.; Sugumar, P.; Maheshwaran, V.; Ponnuswamy, M. N.; Ponnuswamy, S.

    2016-07-01

    In this study, a series of variously substituted r-2,c-7-diaryl-1,4-diazepan-5-ones 9-16 have been synthesized using Schmidt rearrangement and are characterized by IR, mass and 1D & 2D NMR spectral data. The proton NMR coupling constant and estimated dihedral angles reveal that the compounds 9-16 prefer a chair conformation with equatorial orientation of alkyl and aryl groups. Single crystal X-ray structure has been solved for compounds 9 and 11 which also indicates the preference for distorted chair conformation with equatorial orientation of substituents. The compounds 9-16 have been docked with the structure of Methicillin-resistant Staphylococcus aureus (MRSA) and the results demonstrate that compound 10 is having better docking score and glide energy than others and it is comparable to co-crystal ligand. Furthermore, all the compounds have been evaluated for their antibacterial and antioxidant activities. All the compounds show moderate antibacterial activity and only 11 exhibits better activity against S. aures and Escherichia coli. The compounds 11, 13 and 14 exhibit half of the antioxidant power when compared to the BHT and the remaining compounds show moderate activity.

  8. Synthesis, spectral characterization, crystal structure and molecular docking study of 2,7-diaryl-1,4-diazepan-5-ones

    NASA Astrophysics Data System (ADS)

    Sethuvasan, S.; Sugumar, P.; Maheshwaran, V.; Ponnuswamy, M. N.; Ponnuswamy, S.

    2016-07-01

    In this study, a series of variously substituted r-2,c-7-diaryl-1,4-diazepan-5-ones 9-16 have been synthesized using Schmidt rearrangement and are characterized by IR, mass and 1D & 2D NMR spectral data. The proton NMR coupling constant and estimated dihedral angles reveal that the compounds 9-16 prefer a chair conformation with equatorial orientation of alkyl and aryl groups. Single crystal X-ray structure has been solved for compounds 9 and 11 which also indicates the preference for distorted chair conformation with equatorial orientation of substituents. The compounds 9-16 have been docked with the structure of Methicillin-resistant Staphylococcus aureus (MRSA) and the results demonstrate that compound 10 is having better docking score and glide energy than others and it is comparable to co-crystal ligand. Furthermore, all the compounds have been evaluated for their antibacterial and antioxidant activities. All the compounds show moderate antibacterial activity and only 11 exhibits better activity against S. aures and Escherichia coli. The compounds 11, 13 and 14 exhibit half of the antioxidant power when compared to the BHT and the remaining compounds show moderate activity.

  9. Synthesis and anticancer activity of novel 4-morpholino-7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidine derivatives bearing chromone moiety.

    PubMed

    Sun, Chengyu; Chen, Chen; Xu, Shan; Wang, Jianqiang; Zhu, Yan; Kong, Dejia; Tao, Hong; Jin, Mengjia; Zheng, Pengwu; Zhu, Wufu

    2016-08-15

    Herein, we designed and synthesized of a novel series of 7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidine derivatives bearing chromone moiety (10a-j, 13a-j). All the compounds were evaluated for the IC50 values against five cancer cell lines (A549, PC-3, MCF-7, Hela and HepG2). Seven of the target compounds exhibited moderate to excellent cytotoxicity. For these compounds, we tested their inhibitory activities against mTOR kinase, and four of them were tested their inhibitory activities against PI3Kα kinase in further. The results indicated that the optimized compound 10j showed excellent inhibitory activity and cytotoxicity against mTOR kinase, PI3Kα kinase and five cancer cell lines with IC50 values of 1.1μM, 0.92μM and 8.77-14.3μM. Structure-activity relationships (SARs) and docking studies indicated that the thiopyrano[4,3-d]pyrimidine scaffolds exerted little effect on antitumor activities of target compounds. Substitutions of chromone moiety at C-6 position with carboxyl were benefit to the antitumor activities. PMID:27353887

  10. Crystal chemistry of anhydrous Li uranyl phosphates and arsenates. II. Tubular fragments and cation-cation interactions in the 3D framework structures of Li 6[(UO 2) 12(PO 4) 8(P 4O 13)], Li 5[(UO 2) 13(AsO 4) 9(As 2O 7)], Li[(UO 2) 4(AsO 4) 3] and Li 3[(UO 2) 7(AsO 4) 5O)

    NASA Astrophysics Data System (ADS)

    Alekseev, Evgeny V.; Krivovichev, Sergey V.; Depmeier, Wulf

    2009-11-01

    Single crystals of the new compounds Li 6[(UO 2) 12(PO 4) 8(P 4O 13)] ( 1), Li 5[(UO 2) 13(AsO 4) 9(As 2O 7)] ( 2), Li[(UO 2) 4(AsO 4) 3] ( 3) and Li 3[(UO 2) 7(AsO 4) 5O)] ( 4) have been prepared using high-temperature solid state reactions. The crystal structures have been solved by direct methods: 1—monoclinic, C2/ m, a=26.963(3) Å, b=7.063(1) Å, c=19.639(1) Å, β=126.890(4)°, V=2991.2(6) Å 3, Z=2, R1=0.0357 for 3248 unique reflections with | F0|≥4 σ F; 2—triclinic, P1¯, a=7.1410(8) Å, b=13.959(1) Å, c=31.925(1) Å, α=82.850(2)°, β=88.691(2)°, γ=79.774(3)°, V=3107.4(4) Å 3, Z=2, R1=0.0722 for 9161 unique reflections with | F0|≥4 σ F; 3—tetragonal, I4 1/ amd, a=7.160(3) Å, c=33.775(9) Å, V=1732(1) Å 3, Z=4, R1=0.0356 for 318 unique reflections with | F0|≥4 σ F; 4—tetragonal, P4¯, a=7.2160(5) Å, c=14.6540(7) Å, V=763.04(8) Å 3, Z=1, R1=0.0423 for 1600 unique reflections with | F0|≥4 σ F. Structures of all the phases under consideration are based on complex 3D frameworks consisting of different types of uranium polyhedra (UO 6 and UO 7) and different types of tetrahedral TO 4 anions ( T=P or As): PO 4 and P 4O 13 in 1, AsO 4 and As 2O 7 in 2, and single AsO 4 tetrahedra in 3 and 4. In the structures of 1 and 2, UO 7 pentagonal bipyramids share edges to form (UO 5) ∞ chains extended along the b axis in 1 and along the a axis in 2. The chains are linked via single TO 4 tetrahedra into tubular units with external diameters of 11 Å in 1 and 11.5 Å in 2, and internal diameters of 4.1 Å in 1 and 4.5 Å in 2. The channels accommodate Li + cations. The tubular units are linked into 3D frameworks by intertubular complexes. Structures of 3 and 4 are based on 3D frameworks composed on layers united by (UO 5) ∞ infinite chains. Cation-cation interactions are observed in 2, 3, and 4. In 2, the structure contains a trimeric unit with composition [OU(1)O]-U(13)-[OU(2)O]. In the structures of 3 and 4, T-shaped dimers are

  11. Thermal expansion, thermal conductivity, and heat capacity measurements for boreholes UE25 NRG-4, UE25 NRG-5, USW NRG-6, and USW NRG-7/7A

    SciTech Connect

    Brodsky, N.S.; Riggins, M.; Connolly, J.; Ricci, P.

    1997-09-01

    Specimens were tested from four thermal-mechanical units, namely Tiva Canyon (TCw), Paintbrush Tuff (PTn), and two Topopah Spring units (TSw1 and TSw2), and from two lithologies, i.e., welded devitrified (TCw, TSw1, TSw2) and nonwelded vitric tuff (PTn). Thermal conductivities in W(mk){sup {minus}1} averaged over all boreholes, ranged (depending upon temperature and saturation state) from 1.2 to 1.9 for TCw, from 0.4 to 0.9 for PTn, from 1.0 to 1.7 for TSw1, and from 1.5 to 2.3 for TSw2. Mean coefficients of thermal expansion were highly temperature dependent and values, averaged over all boreholes, ranged (depending upon temperature and saturation state) from 6.6 {times} 10{sup {minus}6} to 49 {times} 10{sup {minus}6} C{sup {minus}1} for TCw, from the negative range to 16 {times} 10{sup {minus}6} {center_dot} {degree}C{sup {minus}1} for PTn, from 6.3 {times} 10{sup {minus}6} to 44 {times} 10{sup {minus}6} C{sup {minus}1} for TSw1, and from 6.7 {times} 10{sup {minus}6} to 37 {times} 10{sup {minus}6} {center_dot} {degree}C{sup {minus}1} for TSw2. Mean values of thermal capacitance in J/cm{sup 3}K (averaged overall specimens) ranged from 1.6 J to 2.1 for TSw1 and from 1.8 to 2.5 for TSw2. In general, the lithostratigraphic classifications of rock assigned by the USGS are consistent with the mineralogical data presented in this report.

  12. 5,7-Bis(2'-arylethenyl)-6H-1,4-diazepine-2,3-dicarbonitriles: synthesis, and experimental and theoretical evaluation of the effects of substituents at 5,6,7-positions on the molecular configuration and spectral properties.

    PubMed

    Tarakanov, Pavel A; Simakov, Anton O; Dzuban, Alexander V; Shestov, Vladimir I; Tarakanova, Ekaterina N; Pushkarev, Victor E; Tomilova, Larisa G

    2016-01-21

    A series of novel 5,7-bis(2'-arylethenyl)-6H-1,4-diazepine-2,3-dicarbonitriles was synthesized through sequential aldol condensation reactions of 1,3-diketones with diaminomaleonitrile, and the resulting 5,7-dimethyl-6H-1,4-diazepines were condensed with aromatic aldehydes. The substituents' effects on the spectral properties and conformational states of the molecules in solution were studied using 2D NMR techniques and DFT calculations. Specific intramolecular steric interactions in derivatives substituted at the C6 position were discovered and investigated in detail. Differential scanning calorimetry and thermogravimetric analyses revealed the strong dependence of the thermal stability of the newly prepared diazepinodicarbonitriles on the nature of the substituents. This offers new insight into the structure-property relationships of arylethenyl-substituted diazepine derivatives. PMID:26646741

  13. Km and kcat. values for [6,6,7,7-2H]7,8(6H)-dihydropterin and 2,6-diamino-5-iminopyrimidin-4-one with dihydropteridine reductase.

    PubMed Central

    Armarego, W L; Randles, D; Taguchi, H

    1983-01-01

    The Km and kcat. values for [6,6,7,7-2H]7,8(6H)-dihydropterin and 2,6-diamino-5-iminopyrimidin-4-one were determined for dihydropteridine reductase (EC 1.6.99.10) from two sources. The parameters of the pterin are of the same order as those of the most effective substrates of dihydropteridine reductase. The Km values of the pterin are one order of magnitude smaller than those of the pyrimidinone, although the kcat. values are of the same order. PMID:6870836

  14. Spin dynamics, short range order and spin freezing in Y0.5Ca0.5BaCo4O7

    SciTech Connect

    Stewart, John Ross; Ehlers, Georg; Fouquet, Peter; Mutka, Hannu; Payen, Christophe; Lortz, Rolf

    2011-01-01

    Y0.5Ca0.5BaCo4O7 was recently introduced as a possible candidate for capturing some of the predicted classical spin kagome ground-state features. Stimulated by this conjecture, we have taken up a more complete study of the spin correlations in this compound with neutron scattering methods on a powder sample characterized with high-resolution neutron diffraction and the temperature dependence of magnetic susceptibility and specific heat. We have found that the frustrated near-neighbor magnetic correlations involve not only the kagome planes but concern the full Co sublattice, as evidenced by the analysis of the wave-vector dependence of the short-range order. We conclude from our results that the magnetic moments are located on the Co sublattice as a whole and that correlations extend beyond the two-dimensional kagome planes. We identify intriguing dynamical properties, observing high-frequency fluctuations with a Lorentzian linewidth G?20 meV at ambient temperature. On cooling a low-frequency ({approx}1 meV) dynamical component develops alongside the high-frequency fluctuations, which eventually becomes static at temperatures below T {approx} 50 K. The high-frequency response with an overall linewidth of {approx}10 meV prevails at T?2 K, coincident with a fully elastic short-range-ordered contribution.

  15. Synthesis and pharmacological properties of 1-(4-substituted)butyl derivatives of amides of 7-methyl-3-phenyl-2,4-dioxo-1,2,3,4-tetrahydropyrido[2,3-d]pyrimi dine-5 -carboxylic acid.

    PubMed

    Sladowska, H; Sieklucka-Dziuba, M; Rejdak, R; Kleinrok, Z

    2000-01-01

    The synthesis of 1-(4-substituted)butyl derivatives of amides of 7-methyl-3-phenyl-2,4-dioxo-1,2,3,4-tetrahydropyrido[2,3-d]pyrimid ine-5- carboxylic acid and the results of the preliminary pharmacological screening are described in this paper. Some of them showed a weak analgesic action and caused suppression of the spontaneous locomotor activity of mice. PMID:10755225

  16. 40 CFR 721.5284 - Chromate (5-), bis[4-hydroxy-7-[(2-hydroxy-1-naphthalenyl)azo]- 3-[(2-hydroxy-3-nitro-5...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Chromate (5-), bis - 3- -2... New Uses for Specific Chemical Substances § 721.5284 Chromate (5-), bis - 3- -2- naphthalenesulfonato... chemical substance identified as a Chromate (5-), bis - 3- -2- naphthalenesulfonato(4-)]-, pentasodium...

  17. 40 CFR 721.5284 - Chromate (5-), bis[4-hydroxy-7-[(2-hydroxy-1-naphthalenyl)azo]- 3-[(2-hydroxy-3-nitro-5...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Chromate (5-), bis - 3- -2... New Uses for Specific Chemical Substances § 721.5284 Chromate (5-), bis - 3- -2- naphthalenesulfonato... chemical substance identified as a Chromate (5-), bis - 3- -2- naphthalenesulfonato(4-)]-, pentasodium...

  18. 40 CFR 721.5284 - Chromate (5-), bis[4-hydroxy-7-[(2-hydroxy-1-naphthalenyl)azo]- 3-[(2-hydroxy-3-nitro-5...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Chromate (5-), bis - 3- -2... New Uses for Specific Chemical Substances § 721.5284 Chromate (5-), bis - 3- -2- naphthalenesulfonato... chemical substance identified as a Chromate (5-), bis - 3- -2- naphthalenesulfonato(4-)]-, pentasodium...

  19. 40 CFR 721.5284 - Chromate (5-), bis[4-hydroxy-7-[(2-hydroxy-1-naphthalenyl)azo]- 3-[(2-hydroxy-3-nitro-5...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Chromate (5-), bis - 3- -2... New Uses for Specific Chemical Substances § 721.5284 Chromate (5-), bis - 3- -2- naphthalenesulfonato... chemical substance identified as a Chromate (5-), bis - 3- -2- naphthalenesulfonato(4-)]-, pentasodium...

  20. 40 CFR 721.5284 - Chromate (5-), bis[4-hydroxy-7-[(2-hydroxy-1-naphthalenyl)azo]- 3-[(2-hydroxy-3-nitro-5...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Chromate (5-), bis - 3- -2... New Uses for Specific Chemical Substances § 721.5284 Chromate (5-), bis - 3- -2- naphthalenesulfonato... chemical substance identified as a Chromate (5-), bis - 3- -2- naphthalenesulfonato(4-)]-, pentasodium...

  1. Eaf5/7/3 form a functionally independent NuA4 submodule linked to RNA polymerase II-coupled nucleosome recycling

    PubMed Central

    Rossetto, Dorine; Cramet, Myriam; Wang, Alice Y; Steunou, Anne-Lise; Lacoste, Nicolas; Schulze, Julia M; Côté, Valérie; Monnet-Saksouk, Julie; Piquet, Sandra; Nourani, Amine; Kobor, Michael S; Côté, Jacques

    2014-01-01

    The NuA4 histone acetyltransferase complex is required for gene regulation, cell cycle progression, and DNA repair. Dissection of the 13-subunit complex reveals that the Eaf7 subunit bridges Eaf5 with Eaf3, a H3K36me3-binding chromodomain protein, and this Eaf5/7/3 trimer is anchored to NuA4 through Eaf5. This trimeric subcomplex represents a functional module, and a large portion exists in a native form outside the NuA4 complex. Gene-specific and genome-wide location analyses indicate that Eaf5/7/3 correlates with transcription activity and is enriched over the coding region. In agreement with a role in transcription elongation, the Eaf5/7/3 trimer interacts with phosphorylated RNA polymerase II and helps its progression. Loss of Eaf5/7/3 partially suppresses intragenic cryptic transcription arising in set2 mutants, supporting a role in nucleosome destabilization. On the other hand, loss of the trimer leads to an increase of replication-independent histone exchange over the coding region of transcribed genes. Taken together, these results lead to a model where Eaf5/7/3 associates with elongating polymerase to promote the disruption of nucleosomes in its path, but also their refolding in its wake. PMID:24843044

  2. The discovery and preclinical characterization of 6-chloro-N-(2-(4,4-difluoropiperidin-1-yl)-2-(2-(trifluoromethyl)pyrimidin-5-yl)ethyl)quinoline-5-carboxamide based P2X7 antagonists.

    PubMed

    Rech, Jason C; Bhattacharya, Anindya; Branstetter, Bryan J; Love, Christopher J; Leenaerts, Joseph E; Cooymans, Ludwig P; Eckert, William A; Ao, Hong; Wang, Qi; Chaplan, Sandra R; Wickenden, Alan D; Lebsack, Alec D; Breitenbucher, J Guy

    2016-10-01

    The synthesis, SAR and preclinical characterization of a series of 6-chloro-N-(2-(4,4-difluoropiperidin-1-yl)-2-(2-(trifluoromethyl)pyrimidin-5-yl)ethyl)quinoline-5-carboxamide based P2X7 antagonists is described herein. The lead compounds are potent inhibitors in Ca(2+) flux and whole blood IL-1β P2X7 release assays at both human and mouse isoforms. Compound 1e showed a robust reduction of IL-1β release in a mouse ex vivo model with a 50mg/kg oral dose. Evaluation of compound 1e in the mouse SNI tactile allodynia, carrageenan-induced paw edema or CIA models resulted in no analgesic or anti-inflammatory effects. PMID:27595421

  3. Turtle sex determination assay: Mass balance and responses to 2,3,7,8-tetrachlorodibenzo-p-dioxin and 3,3',4,4',5-pentachlorobiphenyl

    USGS Publications Warehouse

    Gale, Robert W.; Bergeron, Judith M.; Willingham, Emily J.; Crews, David

    2002-01-01

    Polyhalogenated hydrocarbons have been implicated in the anomalous sexual differentiation of mammals and reptiles. Here, a temperature-sensitive turtle sex determination assay using the red-eared slider (Trachemys scripta elegans) was used to determine the estrogenic or antiestrogenic activity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3′,4,4′,5-pentachlorobiphenyl (PCB-126). Neither TCDD nor PCB-126 showed a statistically significant difference in the resulting sex ratios (Fisher's exact test, p < 0.45). As a consequence of the dosing technique (eggshell spotting), the shell retained 90 and 96% of the dose for PCB-126 and TCDD, respectively, similar to retention of estradiol-17β. However, the dosing allowed transfer of sufficient chemical to achieve tissue concentrations that were greater than most concentrations reported for environmentally incurred residues. Similar relative mass distributions of PCB-126 and TCDD were observed in albumin (14–20%), yolk (55–70%), and embryo (16–25%). Relative concentration distributions in the embryo approached those in the yolk, 37 to 40% and 40 to 52%, respectively, while relative concentrations in the albumin remained at 11 to 20%. Lipid-normalized TCDD and PCB-126 concentrations were 30- to 40-fold greater in the embryo than in the yolk. It is hypothesized that nonpassive partitioning processes may have occurred in the embryo.

  4. Design and performance of a 4.5GHz circularly polarized YBa 2Cu 3O 7 microstrip antenna

    NASA Astrophysics Data System (ADS)

    Zhu, M. H.; Cao, B. S.; Zhang, X. X.; Li, W. H.; Yuan, H. J.; Wang, Y. J.; Zhang, L. W.; Dong, D. J.; Liu, M. L.; Cui, D. F.; He, M.; Zhou, Y. L.; Liu, T. J.

    1997-08-01

    A 4.5GHz circularly polarized YBCO microstrip antenna was designed and fabricated. Measurements showed that at 77K the superconducting antenna had about 3dB gain improvement over the comparable silver antenna, in agreement with the calculated results using the modified Green function method.

  5. Relaxation of the V = 4,5,6,7, Sigma g/+/ vibrational levels of carbon monoxide studied by laser absorption.

    NASA Technical Reports Server (NTRS)

    Chackerian, C., Jr.; Weisbach, M. F.

    1973-01-01

    The vibrational relaxation of individual vibration rotation levels of carbon monoxide behind incident shock waves of carbon monoxide has been studied by the method of laser absorption. For the particular vibrational states (V = 4 to 7) and temperature range (2500 to 5500 K) studied, it is concluded that the characteristic relaxation times are in excellent agreement with those obtained via previous measurements of 'bulk' gas properties. Further, the data furnish strong corroboration of the idea that the individual levels are in Boltzmann vibrational equilibrium during the relaxation process.

  6. Reaction of (chloro carbonyl) phenyl ketene with 5-amino pyrazolones: Synthesis, characterization and theoretical studies of 7-hydroxy-6-phenyl-3-(phenyldiazenyl)pyrazolo[1,5-a]pyrimidine-2,5(1H,4H)-dione derivatives

    NASA Astrophysics Data System (ADS)

    Zahedifar, Mahboobeh; Razavi, Razieh; Sheibani, Hassan

    2016-12-01

    New 7-hydroxy-6-phenyl-3-(phenyldiazenyl)pyrazolo[1,5-a]pyrimidine-2,5(1H,4H)-dione derivatives were synthesized from the reaction of (chlorocarbonyl)phenyl ketene and 5-amino pyrazolones in high to excellent yields and short reaction times. Structures of the new compounds were fully characterized by their spectral data IR, 1H NMR, and 13C NMR and by the theoretical results. Density Functional Theory (DFT) was used to optimize the structures, compute the energies and vibrational frequencies IR and 1H NMR shielding tensors of the desired products. The theoretical results excellent are compared with the experimental data.

  7. cis-trans photoisomerization of 1,3,5,7-octatetraene in n-hexane at 4.2 K

    PubMed Central

    Granville, Mark F.; Holtom, Gary R.; Kohler, Bryan E.

    1980-01-01

    Photoisomerization of the linear polyene 1,3,5,7-octatetraene has been observed in an n-hexane matrix maintained at the boiling point of helium. To a good approximation, only the trans,trans and cis,trans isomers participate in the photochemistry. These compounds have been unambiguously identified by comparing the observed high-resolution fluorescence spectra to those of chromatographically purified reference compounds. Although the quantum yield of this process is probably low, its microscopic rate seems to compete favorably with vibrational deactivation. PMID:16592751

  8. Ins(1,4,5)P3 interacts with PIP2 to regulate activation of TRPC6/C7 channels by diacylglycerol in native vascular myocytes

    PubMed Central

    Ju, Min; Shi, Jian; Saleh, Sohag N; Albert, Anthony P; Large, William A

    2010-01-01

    We investigated synergism between inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and diacylglycerol (DAG) on TRPC6-like channel activity in rabbit portal vein myocytes using single channel recording and immunoprecipitation techniques. Ins(1,4,5)P3 at 10 μm increased 3-fold TRPC6-like activity induced by 10 μm 1-oleoyl-2-acetyl-sn-glycerol (OAG), a DAG analogue. Ins(1,4,5)P3 had no effect on OAG-induced TRPC6 activity in mesenteric artery myocytes. Anti-TRPC6 and anti-TRPC7 antibodies blocked channel activity in portal vein but only anti-TRPC6 inhibited activity in mesenteric artery. TRPC6 and TRPC7 proteins strongly associated in portal vein but only weakly associated in mesenteric artery tissue lysates. Therefore in portal vein the conductance consists of TRPC6/C7 subunits, while OAG activates a homomeric TRPC6 channel in mesenteric artery myocytes. Wortmannin at 20 μm reduced phosphatidylinositol 4,5-bisphosphate (PIP2) association with TRPC6 and TRPC7, and produced a 40-fold increase in OAG-induced TRPC6/C7 activity. Anti-PIP2 antibodies evoked TRPC6/C7 activity, which was blocked by U73122, a phospholipase C inhibitor. DiC8-PIP2, a water-soluble PIP2 analogue, inhibited OAG-induced TRPC6/C7 activity with an IC50 of 0.74 μm. Ins(1,4,5)P3 rescued OAG-induced TRPC6/C7 activity from inhibition by diC8-PIP2 in portal vein myocytes, and this was not prevented by the Ins(1,4,5)P3 receptor antagonist heparin. In contrast, Ins(1,4,5)P3 did not overcome diC8-PIP2-induced inhibition of TRPC6 activity in mesenteric artery myocytes. 2,3,6-Tri-O-butyryl-Ins(1,4,5)P3/AM (6-Ins(1,4,5)P3), a cell-permeant analogue of Ins(1,4,5)P3, at 10 μm increased TRPC6/C7 activity in portal vein and reduced association between TRPC7 and PIP2, but not TRPC6 and PIP2. In contrast, 10 μm OAG reduced association between TRPC6 and PIP2, but not between TRPC7 and PIP2. The present work provides the first evidence that Ins(1,4,5)P3 modulates native TRPC channel activity through removal of the

  9. Accelerators (4/5)

    ScienceCinema

    None

    2011-10-06

    1a) Introduction and motivation 1b) History and accelerator types 2) Transverse beam dynamics 3a) Longitudinal beam dynamics 3b) Figure of merit of a synchrotron/collider 3c) Beam control 4) Main limiting factors 5) Technical challenges Prerequisite knowledge: Previous knowledge of accelerators is not required.

  10. Atmospheric histories and growth trends of C4F10, C5F12, C6F14, C7F16 and C8F18

    NASA Astrophysics Data System (ADS)

    Ivy, D. J.; Arnold, T.; Harth, C. M.; Steele, L. P.; Mühle, J.; Rigby, M.; Salameh, P. K.; Leist, M.; Krummel, P. B.; Fraser, P. J.; Weiss, R. F.; Prinn, R. G.

    2012-05-01

    Atmospheric observations and trends are presented for the high molecular weight perfluorocarbons (PFCs): decafluorobutane (C4F10), dodecafluoropentane (C5F12), tetradecafluorohexane (C6F14), hexadecafluoroheptane (C7F16) and octadecafluorooctane (C8F18). Their atmospheric histories are based on measurements of 36 Northern Hemisphere and 46 Southern Hemisphere archived air samples collected between 1973 to 2011 using the Advanced Global Atmospheric Gases Experiment (AGAGE) "Medusa" preconcentration gas chromatography-mass spectrometry systems. A new calibration scale was prepared for each PFC, with estimated accuracies of 6.8% for C4F10, 7.8% for C5F12, 4.0% for C6F14, 6.6% for C7F16 and 7.9% for C8F18. Based on our observations the 2011 globally averaged dry air mole fractions of these heavy PFCs are: 0.17 parts-per-trillion (ppt, i.e., parts per 1012) for C4F10, 0.12 ppt for C5F12, 0.27 ppt for C6F14, 0.12 ppt for C7F16 and 0.09 ppt for C8F18. These atmospheric mole fractions combine to contribute to a global average radiative forcing of 0.35 mW m-2, which is 6% of the total anthropogenic PFC radiative forcing (Montzka and Reimann, 2011; Oram et al., 2012). The growth rates of the heavy perfluorocarbons were largest in the late 1990s peaking at 6.2 parts per quadrillion (ppq, i.e., parts per 1015) per year (yr) for C4F10, at 5.0 ppq yr-1 for C5F12 and 16.6 ppq yr-1 for C6F14 and in the early 1990s for C7F16 at 4.7 ppq yr-1 and in the mid 1990s for C8F18 at 4.8 ppq yr-1. The 2011 globally averaged mean atmospheric growth rates of these PFCs are subsequently lower at 2.2 ppq yr-1 for C4F10, 1.4 ppq yr-1 for C5F12, 5.0 ppq yr-1 for C6F14, 3.4 ppq yr-1 for C7F16 and 0.9 ppq yr-1 for C8F18. The more recent slowdown in the growth rates suggests that emissions are declining as compared to the 1980s and 1990s.

  11. A survey of z > 5.7 quasars in the sloan digital sky survey. 4. discovery of seven additional quasars

    SciTech Connect

    Fan, Xiao-Hui; Strauss, Michael A.; Richards, Gordon T.; Hennawi, Joseph F.; Becker, Robert H.; White, Richard L.; Diamond-Stanic, Aleksandar M.; onley, Jennifer L.D; Jiang, Lin-Hua; Kim, J.Serena; Vestergaard, Marianne; Young, Jason E.; Gunn, James E.; Lupton, Robert H.; Knapp, Gillian R.; Schneider, Donald P.; Brandt, W.N.; Bahcall, Neta A.; Barentine, J.C.; Brinkmann, J.; Brewington, Howard J.; /Arizona U., Astron. Dept. - Steward Observ. /Princeton U. Observ. /Johns Hopkins U. /UC, Berkeley, Astron. Dept. /UC, Davis /LLNL, Livermore /Baltimore, Space Telescope Sci. /Penn State U., Astron. Astrophys. /Apache Point Observ. /Tokyo U., ICRR /Mt. Suhora Observ., Cracow /Fermilab /Garching, Max Planck Inst., MPE

    2005-12-01

    We present the discovery of seven quasars at z > 5.7, selected from {approx}2000 deg{sup 2} of multicolor imaging data of the Sloan Digital Sky Survey (SDSS). The new quasars have redshifts z from 5.79 to 6.13. Five are selected as part of a complete flux-limited sample in the SDSS Northern Galactic Cap; two have larger photometric errors and are not part of the complete sample. One of the new quasars, SDSS J1335+3533 (z = 5.93), exhibits no emission lines; the 3-{sigma} limit on the rest-frame equivalent width of Ly{alpha} + NV line is 5 {angstrom}. It is the highest redshift lineless quasar known, and could be a gravitational lensed galaxy, a BL Lac object or a new type of quasar. Two new z > 6 quasars, SDSS 1250+3130 (z = 6.13) and SDSS J1137+3549 (z = 6.01), show deep Gunn-Peterson absorption gaps in Ly{alpha}. These gaps are narrower the complete Gunn-Peterson absorption troughs observed among quasars at z > 6.2 and do not have complete Ly{beta} absorption.

  12. 8-(3-chloro-4-methoxybenzyl)-8H-pyrido[2,3-d]pyrimidin-7-one derivatives as potent and selective phosphodiesterase 5 inhibitors.

    PubMed

    Sakamoto, Toshiaki; Koga, Yuichi; Hikota, Masataka; Matsuki, Kenji; Mochida, Hideki; Kikkawa, Kohei; Fujishige, Kotomi; Kotera, Jun; Omori, Kenji; Morimoto, Hiroshi; Yamada, Koichiro

    2015-04-01

    A novel series of highly selective phosphodiesterase 5 (PDE5) inhibitors was found. 8H-Pyrido[2,3-d]pyrimidin-7-one derivatives bearing an (S)-2-(hydroxymethyl)pyrrolidin-1-yl group at the 2-position and a 3-chloro-4-methoxybenzyl group at the 8-position exhibited potent PDE5 inhibitory activities and high PDE5 selectivity over PDE6. Among the synthesized compounds, the 5-methyl analogue (5b) showed the most potent relaxant effect on isolated rabbit corpus cavernosum with an EC30 value of 0.85 nM. PMID:25754491

  13. Installation Restoration Program (IRP) for IRP sites numbers 4, 5, 7 and 14. 152 Tactical Reconnaissance Group, Nevada Air National Guard, Reno Tahoe International Airport, Reno, Nevada

    SciTech Connect

    1996-01-01

    Remedial Investigation Report for IRP Site Nos. 4,5,7, and 14, Nevada Air National Guard, 152nd Tactical Reconnaissance Group, Reno Tahoe International Airport, Reno, Nevada. This is the remedial investigation report. The sites were investigated under the Installation Restoration Program. Soil and groundwater samples were collected and analyzed. An Engineering Evaluation/Cost Analysis was recommended to fully delineate the extent of contamination and conduct remediation activities, if required for sites 4,5,7, and 14. Groundwater monitoring was recommended for the all sites.

  14. Penta­cobalt(II) divanadium(III) tetrakis(diphosphate), Co5V2(P2O7)4

    PubMed Central

    Bronova, Anna; Glaum, Robert; Litterscheid, Christian

    2013-01-01

    Co5V2(P2O7)4 was crystallized by chemical vapour transport using HCl as transport agent. Its crystal structure is isotypic to that of FeII 5FeIII 2(P2O7)4 and can be regarded as a member of the thortveitite structure family with corrugated layers of metal–oxygen polyhedra extending parallel to (010). Significant occupational disorder between cobalt(II) and vanadium(III) is observed. Four of the five cation sites are occupied by both cobalt and vanadium. The fifth cation site (Co1) is occupied by cobalt only. Sites Co1, M3 and M4 are located on twofold axes. Sites Co1, M2, M3 and M4 show o­cta­hedral coordination by oxygen; M5 has a square-pyramidal environment. PMID:23723750

  15. Practical synthesis of [n]cycloparaphenylenes (n = 5, 7-12) by H2SnCl4-mediated aromatization of 1,4-dihydroxycyclo-2,5-diene precursors.

    PubMed

    Patel, Vijay Kumar; Kayahara, Eiichi; Yamago, Shigeru

    2015-04-01

    Cyclic precursors of cycloparaphenylenes (CPPs) containing 1,4-dihydroxy-2,5-cyclohexadien-1,4-diyl units are prepared by modifying a synthetic method developed by Jasti and co-workers for the synthesis of corresponding 1,4-dimethoxy derivatives. Reductive aromatization of the diyl moieties by SnCl2/2 HCl takes place under mild conditions and affords the CPPs in good yields, incorporating 5 or 7-12 phenylene units. Highly strained [5]CPP is synthesized in greater than 0.3 g scale. (119)Sn NMR spectroscopy clarifies the in situ formation of an ate complex, H2SnCl4, upon mixing a 2:1 ratio of HCl and SnCl2, which serves as a highly active reducing agent under nearly neutral conditions. When more than 2 equivalents of HCl, in relation to SnCl2, are used, acid-catalyzed decomposition of the CPP precursors takes place. The stoichiometry of HCl and SnCl2 is critical in achieving the desired aromatization reaction of highly strained CPP precursors. PMID:25753916

  16. The thermoluminescent dose response of glow peaks 4, 5, and 7 in (LiF: Mg, Ti) for measuring occupational exposure to neutrons

    NASA Astrophysics Data System (ADS)

    Macievic, Gregory Vincent

    The objective of this research thesis was to determine the feasibility of using a single thermoluminescent dosimeter (TLD) to measure occupational exposure from a mixed radiation field of fast neutrons and photons. Glow curve analysis was used to characterize the response of sp6LiF Thermoluminescent Dosimeters after controlled exposure to a mixed field radiation dose. The intensities of thermoluminescent (TL) glow peak 7 and glow peak 4 were investigated relative to the main TL glow peak (number 5) to distinguish between neutron and gamma ray exposure. The analysis was accomplished by exposing TLDs to a known source of monoenergetic and continuous spectra neutrons in a gamma ray field. The results of these analyses are: (1) There is a distinct difference in channel position of peak 4 by radiation type. (2) Gamma rays severely confound the analysis of peak 7 by reducing or eliminating the neutron exposure if the gamma exposure occurs after neutron exposure. In addition, peaks of dosimeters first exposed to pure neutrons are severely reduced or eliminated when the dosimeters are later exposed to gamma rays in neutron-to-gamma ratios of less than 3. (3) The peak height and integral value ratios (for PK4/PK5 and PK7/PK5) provide a useful means of neutron/gamma discrimination. (4) An algorithm was developed for the californium dose computation using the ratios of peaks 4, 5, and 7: D = 1.10lbrack 10.12 - Isb5(1/Isb7 + 1/Isb4)rbrack - 0.05 in rem.

  17. (2R)-4-Oxo-4[3-(Trifluoromethyl)-5,6-diihydro:1,2,4}triazolo[4,3-a}pyrazin-7(8H)-y1]-1-(2,4,5-trifluorophenyl)butan-2-amine: A Potent, Orally Active Dipeptidyl Peptidase IV Inhibitor for the Treatment of Type 2 Diabetes

    SciTech Connect

    Kim, D.; Wang, L.; Beconi, M.; Eiermann, G.; Fisher, M.; He, H.; Hickey, G.; Kowalchick, Jennifer; Leiting, Barbara; Lyons, K.; Marsilio, F.; McCann, F.; Patel, R.; Petrov, A.; Scapin, G.; Patel, S.; Roy, R.; Wu, J.; Wyvratt, M.; Zhang, B.; Zhu, L.; Thornberry, N.; Weber, A.

    2010-11-10

    A novel series of {beta}-amino amides incorporating fused heterocycles, i.e., triazolopiperazines, were synthesized and evaluated as inhibitors of dipeptidyl peptidase IV (DPP-IV) for the treatment of type 2 diabetes. (2R)-4-Oxo-4-[3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-1-(2,4,5-trifluorophenyl)butan-2-amine (1) is a potent, orally active DPP-IV inhibitor (IC{sub 50} = 18 nM) with excellent selectivity over other proline-selective peptidases, oral bioavailability in preclinical species, and in vivo efficacy in animal models. MK-0431, the phosphate salt of compound 1, was selected for development as a potential new treatment for type 2 diabetes.

  18. Substituted pyrazoles as hepatoselective HMG-CoA reductase inhibitors: discovery of (3R,5R)-7-[2-(4-fluoro-phenyl)-4-isopropyl-5-(4-methyl-benzylcarbamoyl)-2H-pyrazol-3-yl]-3,5-dihydroxyheptanoic acid (PF-3052334) as a candidate for the treatment of hypercholesterolemia.

    PubMed

    Pfefferkorn, Jeffrey A; Choi, Chulho; Larsen, Scott D; Auerbach, Bruce; Hutchings, Richard; Park, William; Askew, Valerie; Dillon, Lisa; Hanselman, Jeffrey C; Lin, Zhiwu; Lu, Gina H; Robertson, Andrew; Sekerke, Catherine; Harris, Melissa S; Pavlovsky, Alexander; Bainbridge, Graeme; Caspers, Nicole; Kowala, Mark; Tait, Bradley D

    2008-01-10

    In light of accumulating evidence that aggressive LDL-lowering therapy may offer increased protection against coronary heart disease, we undertook the design and synthesis of a novel series of HMG-CoA reductase inhibitors based upon a substituted pyrazole template. Optimizing this series using both structure-based design and molecular property considerations afforded a class of highly efficacious and hepatoselective inhibitors resulting in the identification of (3 R,5 R)-7-[2-(4-fluoro-phenyl)-4-isopropyl-5-(4-methyl-benzylcarbamoyl)-2 H-pyrazol-3-yl]-3,5-dihydroxy-heptanoic (PF-3052334) as a candidate for the treatment of hypercholesterolemia. PMID:18072721

  19. Cross-section measurement for the 10B(n, alpha)7Li reaction at 4.0 and 5.0 MeV.

    PubMed

    Zhang, Guohui; Guo, Li'an; Cao, Rongtai; Zhang, Jiaguo; Chen, Jinxiang

    2008-10-01

    Cross-sections of the (10)B(n, alpha)(7)Li reaction were measured at En=4.0 and 5.0 MeV. A gridded ionization chamber (GIC) was used as charged particle detector. Neutrons were produced through the D(d, n)(3)He reaction with a deuterium gas target. Experiments were performed at the 4.5 MV Van de Graaff accelerator of Peking University. Cross-section data of the (238)U(n, f) reaction were employed as standard. The measured cross-sections of the (10)B(n, alpha)(7)Li reaction at 4.0 and 5.0 MeV are 211+/-17 and 169+/-14 mb, respectively, and they are compared with existing results of measurements and evaluations. PMID:18387305

  20. On the reactivity of 6-acetyl-7-(2-dimethylaminovinyl)pyrazolo[1,5-a]pyrimidines with 1,3- and 1,4-bisnucleophiles.

    PubMed

    Chimichi, Stefano; Boccalini, Marco; Selleri, Silvia; Costagli, Camilla; Guerrini, Gabriella; Viola, Giampietro

    2008-02-21

    Reaction of 6-acetyl-7-(2-dimethylaminovinyl)pyrazolo[1,5-a]pyrimidine 1 with 1,3- and 1,4-bisnucleophiles has been investigated; obtainment of new polycyclic heterocyclic derivatives is reported. A convenient procedure leading to new pyrazolo[1,5-a]quinazolines is described; a modest bioactivity of these compounds against two human tumor cell lines was also ascertained. PMID:18264575

  1. Atmospheric histories and growth trends of C4F10, C5F12, C6F14, C7F16 and C8F18

    NASA Astrophysics Data System (ADS)

    Ivy, D. J.; Arnold, T.; Harth, C. M.; Steele, L. P.; Mühle, J.; Rigby, M.; Salameh, P. K.; Leist, M.; Krummel, P. B.; Fraser, P. J.; Weiss, R. F.; Prinn, R. G.

    2012-02-01

    The first atmospheric observations and trends are presented for the high molecular weight perfluorocarbons (PFCs): decafluorobutane (C4F10), dodecafluoropentane (C5F12), tetradecafluorohexane (C6F14), hexadecafluoroheptane (C7F16) and octadecafluorooctane (C8F18). Their atmospheric histories are based on measurements of 38 Northern Hemisphere and 46 Southern Hemisphere archived air samples collected between 1973 to 2011 using the Advanced Global Atmospheric Gases Experiment (AGAGE) "Medusa" preconcentration gas chromatography-mass spectrometry systems. A new calibration scale was prepared for each PFC, with estimated accuracies of 6.8% for C4F10, 7.8% for C5F12, 4.0% for C6F14, 6.6% for C7F16 and 7.9% for C8F18. Based on our observations the 2011 globally averaged dry air mole fractions of these heavy PFCs are: 0.18 parts-per-trillion (ppt, i.e., parts per 1012) for C4F10, 0.12 ppt for C5F12, 0.28 ppt for C6F14, 0.12 ppt for C7F16 and 0.09 ppt for C8F18. These atmospheric mole fractions combine to contribute to a global average radiative forcing of 0.35 mW m-2, which is 3.6% of the total PFC radiative forcing. The globally averaged mean atmospheric growth rates of these PFCs during 1973-2011 are 4.58 parts per quadrillion (ppq, i.e., parts per 1015) per year (yr) for C4F10, 3.29 ppq yr-1 for C5F12, 7.50 ppq yr-1 for C6F14, 3.19 ppq yr-1 for C7F16 and 2.51 ppq yr-1 for C8F18. The growth rates of the heavy perfluorocarbons were largest in the early 1990s for C4F10 and C5F12 and in the mid-to-late 1990s for C6F14, C7F16 and C8F18. The more recent slow down in the growth rates of the high molecular weight PFCs suggests that emissions are declining as compared to the 1980s and 1990s. Nevertheless continued monitoring of these potent, extremely long-lived greenhouse gases is necessary to verify that global PFC emissions continue to decline.

  2. Platinum(IV) coordination compounds containing 5-methyl-1,2,4-triazolo[1,5- a]pyrimidin-7(4 H)-one as nonleaving ligand. Molecular and cytotoxicity in vitro characterization

    NASA Astrophysics Data System (ADS)

    Łakomska, Iwona; Fandzloch, Marzena; Wojtczak, Andrzej; Szłyk, Edward

    2011-08-01

    Novel platinum(IV) coordination compounds with 5-methyl-1,2,4-triazolo[1,5- a]pyrimidin-7(4 H)-one (HmtpO): cis- trans-[PtCl 2(OH) 2(NH 3)(HmtpO)] ( 1), cis- trans-[PtCl 5(HmtpO)][(CH 3) 2NH 2] ( 2) have been prepared and structurally characterized by spectroscopic methods ( 1H, IR and X-ray crystallography ( 2)). The X-ray results indicate that the local geometry around the platinum(IV) centre approximates a typical octahedral arrangement with nitrogen atom N3 of the HmtpO and three chloride atoms in equatorial positions. The remaining two axial positions are occupied by two chlorides. The preliminary assessment of antitumor properties of ( 1) was performed as an in vitro antiproliferative activity against HL-60 human acute promyelocytic leukemia and HCV29T bladder cancer. The cis- trans-[PtCl 2(OH) 2(NH 3)(HmtpO)] ( 1) exhibits higher cytotoxic activity against HL-60 (IC 50 = 6.4 μM) than cisplatin.

  3. A facile access to new diazepines derivatives: Spectral characterization and crystal structures of 7-(thiophene-2-yl)-5-(trifluoromethyl)-2,3-dihydro-1H-1,4-diazepine and 2-thiophene-4-trifluoromethyl-1,5-benzodiazepine

    NASA Astrophysics Data System (ADS)

    Ahumada, Guillermo; Carrillo, David; Manzur, Carolina; Fuentealba, Mauricio; Roisnel, Thierry; Hamon, Jean-René

    2016-12-01

    The one-pot double condensation reaction of 2-thenoyltrifluoroacetone (2-TTA) with ethylendiamine or o-phenylenediamine, in a 2:1 stoichiometric molar ratio, leads to the formation of 7-(thiophene-2-yl)-5-(trifluoromethyl)-2,3-dihydro-1H-1,4-diazepine 2 and 2-thiophene-4-trifluoromethyl-1,5-benzodiazepine 3, that were isolated in 56 and 53% yields, respectively. The bis(trifluoroacetamide)ethylene derivative 1 was also isolated in 32% yield as a side-product in the reaction of 2-TTA and ethylenediamine. Compounds 1-3 were fully characterized by elemental analysis, FT-IR and multinuclear (1H, 13C and 19F) NMR spectroscopy. In addition, their molecular identities and geometries have been authenticated by single-crystal X-ray diffraction analysis. The spectroscopic and structural data confirm that the 1,4-diazepine 2 and the 1,5-benzodiazepine 3 exist in the imine-enamine and diimine tautomeric forms, respectively, both in solution and in the solid-state.

  4. Vasorelaxant and antiplatelet activity of 4,7-dimethyl-1,2, 5-oxadiazolo[3,4-d]pyridazine 1,5,6-trioxide: role of soluble guanylate cyclase, nitric oxide and thiols.

    PubMed

    Kots, A Y; Grafov, M A; Khropov, Y V; Betin, V L; Belushkina, N N; Busygina, O G; Yazykova, M Y; Ovchinnikov, I V; Kulikov, A S; Makhova, N N; Medvedeva, N A; Bulargina, T V; Severina, I S

    2000-03-01

    1. Certain heterocyclic N-oxides are vasodilators and inhibitors of platelet aggregation. The pharmacological activity of the furoxan derivative condensed with pyridazine di-N-oxide 4,7-dimethyl-1,2, 5-oxadiazolo[3,4-d]pyridazine 1,5,6-trioxide (FPTO) and the corresponding furazan (FPDO) was studied. 2. FPTO reacted with thiols generating nitrite (NO), S-nitrosoglutathione and hydroxylamine (nitroxyl) and converted oxyHb to metHb. FPDO did not generate detectable amounts of NO-like species but reacted with thiols and oxyHb. 3. FPTO and FPDO haem-dependently stimulated the activity of soluble guanylate cyclase (sGC) and this stimulation was inhibited by 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) and by 0.1 mM dithiothreitol. 4. FPTO relaxed noradrenaline-precontracted aortic rings and its concentration-response curve was biphasic (pIC(50)=9. 03+/-0.13 and 5.85+/-0.06). FPDO was significantly less potent vasodilator (pIC(50)=5.19+/-0.14). The vasorelaxant activity of FPTO and FPDO was inhibited by ODQ. oxyHb significantly inhibited only FPTO-dependent relaxation. 5. FPTO and FPDO were equipotent inhibitors of ADP-induced platelet aggregation (IC(50)=0.63+/-0.15 and 0.49+/-0. 05 microM, respectively). The antiplatelet activity of FPTO (but not FPDO) was partially suppressed by oxyHb. The antiaggregatory effects of FPTO and FPDO were only partially blocked by sGC inhibitors. 6. FPTO and FPDO (10 - 20 microM) significantly increased cyclic GMP levels in aortic rings and platelets and this increase was blocked by ODQ. 7. Thus, FPTO can generate NO and, like FPDO, reacts with thiols and haem. The vasorelaxant activity of FPTO and FPDO is sGC-dependent and a predominant role is played by NO at FPTO concentrations below 1 microM. On the contrary, inhibition of platelet aggregation is only partially related to sGC activation. PMID:10725265

  5. How Slow Can We Go? 4 Frames Per Second (fps) Versus 7.5 fps Fluoroscopy for Atrial Septal Defects (ASDs) Device Closure.

    PubMed

    Hiremath, Gurumurthy; Meadows, Jeffery; Moore, Phillip

    2015-06-01

    Radiation exposure remains a significant concern for ASD device closure. In an effort to reduce radiation exposure, the default fluoroscopy frame rate in our Siemens biplane pediatric catheterization laboratory was reduced to 4 fps in November 2013 from an earlier 7.5 fps fluoro rate. This study aims to evaluate the components contributing to total radiation exposure and compare the procedural success and radiation exposure during ASD device closure using 4 versus 7.5 fps fluoroscopy rates. Twenty ASD device closures performed using 4 fps fluoro rate were weight-matched to 20 ASD closure procedures using 7.5 fps fluoro rate. Baseline characteristics, procedure times and case times were similar in the two groups. Device closure was successful in all but one case in the 4 fps group. The dose area product (DAP), normalized DAP to body weight, total radiation time and fluoro time were lower in the 4 fps group but not statistically different than the 7.5 fps. The number of cine images and cine times were identical in both groups. Fluoroscopy and cineangiography contributed equally to radiation exposure. Fluoroscopy at 4 fps can be safe and effective for ASD device closure in children and adults. There was no increase in procedure time, cine time, fluoro time or complications at this slow fluoro rate. There was a trend toward decreased radiation exposure as measured by indexed DAP although not statistically significant in this small study. Further study with multiple operators using 4 fps fluoroscopy for simple interventional procedures is recommended. PMID:25618164

  6. 4 CFR 5.4 - Pay administration.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 4 Accounts 1 2010-01-01 2010-01-01 false Pay administration. 5.4 Section 5.4 Accounts GOVERNMENT ACCOUNTABILITY OFFICE PERSONNEL SYSTEM COMPENSATION § 5.4 Pay administration. The provisions of chapter 55 of title 5, U.S. Code and the Office of Personnel Management implementing regulations apply to...

  7. 4 CFR 5.4 - Pay administration.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 4 Accounts 1 2013-01-01 2013-01-01 false Pay administration. 5.4 Section 5.4 Accounts GOVERNMENT ACCOUNTABILITY OFFICE PERSONNEL SYSTEM COMPENSATION § 5.4 Pay administration. The provisions of chapter 55 of title 5, U.S. Code and the Office of Personnel Management implementing regulations apply to...

  8. 4 CFR 5.4 - Pay administration.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 4 Accounts 1 2014-01-01 2013-01-01 true Pay administration. 5.4 Section 5.4 Accounts GOVERNMENT ACCOUNTABILITY OFFICE PERSONNEL SYSTEM COMPENSATION § 5.4 Pay administration. The provisions of chapter 55 of title 5, U.S. Code and the Office of Personnel Management implementing regulations apply to...

  9. Adenosine deaminase inhibitors. Synthesis and biological evaluation of (+/-)-3,6,7,8-tetrahydro-3-[(2-hydroxyethoxy)methyl]imidazo[4,5-d] [1,3]diazepin-8-ol and some selected C-5 homologues of pentostatin.

    PubMed

    Showalter, H D; Putt, S R; Borondy, P E; Shillis, J L

    1983-10-01

    The synthesis of several analogues of (8R)-3-(2-deoxy-beta-D-erythro- pentofuranosyl)-3,6,7,8-tetrahydroimidazo[4,5-d][1,3]diazepin-8-ol (pentostatin, 1a) is described. Ring closure of 2-amino-1-(5-amino-1H-imidazol-4-yl)ethanone dihydrochloride (3) with triethyl orthoacetate or triethyl orthopropionate gave the C-5 methyl and ethyl ketoaglycons, 6,7-dihydro-5-methylimidazo[4,5-d][1,3]diazepin-8(3H)-one (4b) and 5-ethyl-6,7-dihydroimidazo[4,5-d][1,3]diazepin-8(3H)-one (4c), respectively. Stannic chloride catalyzed condensation of the pertrimethylsilyl derivatives of 4b and 4c with a protected glycosyl halide afforded anomeric mixtures of ketonucleosides 3-(2-deoxy-3,5-di-O-p-toluoyl-beta- and -alpha-D-erythro-pentofuranosyl)-6,7-dihydro-5-methylimidazo[4,5-d] [1,3]diazepin-8(3H)-one (5b and 6b) and 3-(2-deoxy-3,5-di-O-p-toluoyl)-beta- and -alpha-D-erythro-pentofuranosyl)-5-ethyl-6,7-dihydroimidazo[4,5-d]- [1,3]diazepin-8(3H)-one (5c and 6c), respectively. Subsequent separation of the anomers, followed by deprotection and reduction of 5b, 6b, and 5c, afforded the respective 8R and 8S isomers. Stannic chloride catalyzed condensation of pertrimethylsilyl ketoaglycon 4a with 2-(chloromethoxy)-1-(p-toluoyloxy) ethane to give ketonucleoside 6,7-dihydro-3-[[2-(p-toluoyloxy)ethoxy] methyl]imidazo[4,5-d][1,3]diazepin-8(3H)-one (9a) was followed by deprotection to 6,7-dihydro-3[(2-hydroxyethoxy)methyl]imidazo[4,5-d][1,3] diazepin-8(3H)-one (9b) and then reduction to the racemic acyclic pentostatin analogue (+/-)-3,6,7,8-tetrahydro-3-[ (2-hydroxyethoxy)methyl]imidazo[4,5-d][1,3]diazepin-8-ol (2). Ki values for the in vitro adenosine deaminase (EC 3.5.4.4; type I; calf intestinal mucosa) inhibitory activities of 1b, 1c, and 2 were determined to be 1.6 X 10(-8), 1.5 X 10(-6), and 9.8 X 10(-8) M, respectively. When compounds 2 and 9b were tested in combination with vidarabine against herpes simplex virus, type 1, in an HEp-2 plaque reduction assay, only compound 2 was able to

  10. Structure and dielectric properties of solid solutions Bi7Ti4 + x W x Ta1-2 x O21 ( x = 0-0.5)

    NASA Astrophysics Data System (ADS)

    Zubkov, S. V.; Vlasenko, V. G.; Shuvaeva, V. A.; Shevtsova, S. I.

    2016-01-01

    A number of solid solutions Bi7Ti4 + x W x Ta1-2 x O21 ( x = 0-0.5) have been synthesized from oxides by solid-phase reaction. The crystal structure, the electrophysical characteristics, and the microstructure of the prepared ceramic samples have been studied. According to X-ray powder diffraction, all the compounds are single-phase with the structure of mixed-layer Aurivillius phases ( m = 2.5) with the orthorhombic crystal lattice (space group I2 cm, Z = 2). Temperature dependences of the relative permittivity ɛ( T) of the compound have been measured, from which it has been found that the Curie temperature T C of perovskite-like oxides Bi7Ti4 + x W x Ta1-2 x O21 ( x = 0-0.5) decreases linearly as substitution parameter x decreases. The activation energies of charge carriers have been found in different temperature ranges.

  11. Suppression of Adipogenesis by 5-Hydroxy-3,6,7,8,3',4'-Hexamethoxyflavone from Orange Peel in 3T3-L1 Cells.

    PubMed

    Wang, Yu; Lee, Pei-Sheng; Chen, Yi-Fen; Ho, Chi-Tang; Pan, Min-Hsiung

    2016-09-01

    We reported previously that hydroxylated polymethoxyflavones (HPMFs) effectively suppressed obesity in high-fat-induced mouse. In this study, we further investigated the molecular mechanism of action of 5-hydroxy-3,6,7,8,3',4'-hexamethoxyflavone (5-OH-HxMF), one of major HPMFs in orange peel. Treatment of 5-OH-HxMF effectively inhibited lipid accumulation by 55-60% in a dose-dependent manner. The 5-OH-HxMF attenuated adipogenesis through downregulating adipogenesis-related transcription factors such as peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding proteins (C/EBPs), as well as downstream target fatty acid synthase and acetyl-CoA carboxylase (ACC). 5-OH-HxMF activated adenosine monophosphate-activated protein kinase signaling and silent mating type information regulation 1 (SIRTUIN 1 or SIRT1) in 3T3-L1 adipocytes to decrease lipid accumulation. In addition, the inhibition rate of lipid accumulation was compared between 5-OH-HxMF and 3,5,6,7,8,3',4'-heptamethoxyflavone (HpMF). 5-OH-HxMF inhibited lipid accumulation 15-20% more than HpMF did, indicating that hydroxyl group at position 5 can be a key factor in the suppression of adipogenesis. PMID:27542074

  12. Photophysical properties of ESIPT inspired fluorescent 2-(2-hydroxyphenyl)-6-methylimidazo[4,5-f]isoindole-5,7(1H,6H)-dione and its derivative: Experimental and DFT based approach

    NASA Astrophysics Data System (ADS)

    Deshmukh, Mininath S.; Sekar, Nagaiyan

    2015-01-01

    The excited-state intramolecular proton transfer chromophores 2-(2-hydroxyphenyl)-6-methylimidazo[4,5-f]isoindole-5,7(1H,6H)-dione and 2-(4-(diethylamino)-2-hydroxyphenyl)-6-methylimidazo[4,5-f]isoindole-5,7(1H,6H)-dione are synthesized from 4,5-diamino-N-methylphthalimide. The photophysical behavior of the synthesized chromophores was studied using UV-visible and fluorescence spectroscopy in the polar and non-polar solvents. The synthesized o-hydroxyphenyl benzimidazole derivatives are fluorescent and very sensitive to the solvent polarity. These dyes are thermally stable up to 317 °C. Density Functional Theory computations have been used to understand the structural, molecular, electronic and photophysical properties of the chromophores. The experimental absorption and emission wavelengths are in good agreement with the computed vertical excitation and theoretical emission obtained by Density Functional Theory and Time Dependant Density Functional Theory.

  13. rac-Diethyl 9-hy-droxy-9-methyl-7-phenyl-1,4-diaza-spiro-[4.5]decane-6,8-dicarboxyl-ate.

    PubMed

    Maharramov, Abel M; Ismiyev, Arif I; Rashidov, Bahruz A; Rahimova, Gunel M; Allahverdiyev, Mirza A

    2011-01-01

    The title mol-ecule, C(21)H(30)N(2)O(5), is chiral with four stereogenic centres. The crystal is a racemate and consists of enanti-omeric pairs with the relative configuration rac-(6S*,7R*,8R*,9S*). The ethyl fragment of the eth-oxy-carbonyl group at position 6 is disordered in a 0.46 (3):0.54 (3) ratio. The crystal structure features inter-molecular N-H⋯O. Intra-molecular O-H⋯N and N-H⋯O hydrogen bonds also occur. PMID:21522983

  14. Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts

    PubMed Central

    2010-01-01

    Background We have previously shown that MUC5B and MUC7 mucins from saliva of HIV negative individuals inhibit HIV-1 activity by 100% in an in vitro assay. The purpose of this subsequent study was to investigate whether MUC5B and MUC7 from saliva of HIV patients or with full blown AIDS had a similar inhibitory activity against the virus. Methods Salivary MUC5B and MUC7 from HIV patients with different CD4 counts (< 200, 200-400 and > 400) were incubated with HIV-1 prior to infection of the human T lymphoblastoid cell line (CEM SS cells). Cells were then cultured and viral replication was measured by a qualitative p24 antigen assay. The size, charge and immunoreactivity of mucins from HIV negative and positive individuals was also analysed by SDS-PAGE, Western blot and ELISA respectively. Results It was shown that irrespective of their CD4 counts both MUC5B and MUC7 from HIV patients, unlike the MUC5B and MUC7 from HIV negative individuals, did not inhibit HIV-1 activity. Size, charge and immunoreactivity differences between the mucins from HIV negative and positive individuals and among the mucins from HIV patients of different CD4 count was observed by SDS-PAGE, Western blot and ELISA. Conclusions Purified salivary mucins from HIV positive patients do not inhibit the AIDS virus in an in vitro assay. Although the reason for the inability of mucins from infected individuals to inhibit the virus is not known, it is likely that there is an alteration of the glycosylation pattern, and therefore of charge of mucin, in HIV positive patients. The ability to inhibit the virus by aggregation by sugar chains is thus diminished. PMID:20946627

  15. Photoabsorption of the ground state of Ne and of Ne-like Na+, Mg2+, Al3+, Si4+, P5+, S6+, and Cl7+ ions

    NASA Astrophysics Data System (ADS)

    Sakho, I.

    2016-03-01

    Photoabsorption of the 1s2 2s2 2p6 (1S0) ground state of Ne-like ions is presented in this paper. Resonance energies and width of the 2 s 2p6 n p1P1 series of Ne and Ne-like Na+, Mg2+, Al3+, Si4+, P5+, S6+, and Cl7+ ions are reported. Wavelengths of the 2s2 2p6 (1S0) → 2s2 2p5(2P 3 / 2 , 1 / 2) n d transitions in neon-like Na+ ion and of the 2s2 2p6(1S0) → 2 s 2p6 n p1P1 transitions in Ne and in Ne-like Na+, Mg2+, Al3+, Si4+, P5+, S6+, and Cl7+ ions are tabulated. Analysis of the resonances investigated is done in the framework of the LS, jj and JK coupling schemes. All the calculations are made using the Screening constant by unit nuclear charge (SCUNC) formalism. Very good agreement is found between the SCUNC results and various experimental and theoretical literature values and new data for the Ne-like Si4+, P5+, S6+, and Cl7+ ions are listed.

  16. A new family of 1D, 2D and 3D frameworks aggregated from Ni5, Ni4 and Ni7 building units: synthesis, structure, and magnetism.

    PubMed

    Liu, Ya-Hui; Lu, Li-Ping; Zhu, Miao-Li; Feng, Si-Si; Su, Feng

    2016-05-31

    Three new Ni(ii)-clusters based on a Y-shaped ligand (biphenyl-3,4',5-tricarboxylate, H3BPT), [Ni5(HBPT)4(OH)2(H2O)12]n (), [Ni4(BPT)2(OH)2(H2O)6]n·4nH2O (), and [Ni7(BPT)2(1,4-bib)2(OH)6(HCO2)2]n·3nH2O () (1,4-bib = 1,4-bi(1H-imidazol-1-yl)benzene), have been synthesized under solvothermal conditions. They were studied by infrared spectroscopy (IR), single crystal X-ray diffraction, thermogravimetric analysis (TGA), and magnetochemistry. The complexes contain low nuclear Ni-clusters as building units (BUs). Structurally, in , the cluster BUs of [Ni5(μ3-OH)2](8+) can be viewed as two reverse triangles sharing a common vertex, which are connected by the partially deprotonated μ2-η(1):η(1)-HBPT(2-) forming 1D chains. The BUs of [Ni4(μ3-OH)2](6+) clusters in can be considered as two reverse triangles sharing a common edge and extended by deprotonated μ6-η(1):η(1):η(1):η(1):η(2)-BPT(3-) constructing a 2D framework. The 3D framework of complex consists of a [Ni7(μ3-OH)4(R-COO)7(HCO2)3] cluster BUs with fully deprotonated μ5-η(1):η(1):η(1):η(1):η(1):η(1)-BPT(3-) and 1,4-bib ligands. In addition, TGA reveals that the complexes are stable in the range of 293-548 K. Magnetostructural analyses indicate ferromagnetic coupling of J1 = 1.85(3) and J2 = 2.25(4) cm(-1) in and J = 5.76(6) cm(-1) in , whereas magnetic parameters J1 = -2.64(3), J2 = -23.22(19) and J3 = 12.02(5) cm(-1) indicate an alternating magnetic chain (AF/F) in . PMID:27180871

  17. (4R*,4aR*,7aS*)-5-Oxo-6-phenyl-4a,5,6,7,7a,8-hexa-hydro-4H-furo[2,3-f]isoindole-4-carb-oxy-lic acid.

    PubMed

    Horak, Yuriy I; Lytvyn, Roman Z; Zubkov, Fedor I; Nikitina, Eugeniya V; Homza, Yuriy V; Lis, Tadeusz; Kinzhybalo, Vasyl; Obushak, Mykola D

    2013-02-01

    The asymmetric unit of the title compound, C(17)H(15)NO(4), contains two independent mol-ecules with similar geometric parameters. In both mol-ecules, the conformation of the cyclo-hexene ring is half-chair, while the pyrrolidinone ring adopts an envelope conformation with the γ-carbon atom of the α-pyrrolidinone ring as the flap. In the crystal, O-H⋯O hydrogen bonds between the carb-oxylic and carbonyl groups link alternate independent mol-ecules into chains propagating in the b-axis direction. The crystal packing also features weak C-H⋯π inter-actions. PMID:23424547

  18. Global emission estimates and radiative impact of C4F10, C5F12, C6F14, C7F16 and C8F18

    NASA Astrophysics Data System (ADS)

    Ivy, D. J.; Rigby, M.; Baasandorj, M.; Burkholder, J. B.; Prinn, R. G.

    2012-08-01

    Global emission estimates based on new atmospheric observations are presented for the acylic high molecular weight perfluorocarbons (PFCs): decafluorobutane (C4F10), dodecafluoropentane (C5F12), tetradecafluorohexane (C6F14), hexadecafluoroheptane (C7F16) and octadecafluorooctane (C8F18). Emissions are estimated using a 3-dimensional chemical transport model and an inverse method that includes a growth constraint on emissions. The observations used in the inversion are based on newly measured archived air samples that cover a 39-yr period, from 1973 to 2011, and include 36 Northern Hemispheric and 46 Southern Hemispheric samples. The derived emission estimates show that global emission rates were largest in the 1980s and 1990s for C4F10 and C5F12, and in the 1990s for C6F14, C7F16 and C8F18. After a subsequent decline, emissions have remained relatively stable, within 20%, for the last 5 yr. Bottom-up emission estimates are available from the Emission Database for Global Atmospheric Research version 4.2 (EDGARv4.2) for C4F10, C5F12, C6F14 and C7F16, and inventories of C4F10, C5F12 and C6F14 are reported to the United Nations' Framework Convention on Climate Change (UNFCCC) by Annex 1 countries that have ratified the Kyoto Protocol. The atmospheric measurement-based emission estimates are 20 times larger than EDGARv4.2 for C4F10 and over three orders of magnitude larger for C5F12 (with 2008 EDGARv4.2 estimates for C5F12 at 9.6 kg yr-1, as compared to 67±53 t yr-1 as derived in this study). The derived emission estimates for C6F14 largely agree with the bottom-up estimates from EDGARv4.2. Moreover, the C7F16 emission estimates are comparable to those of EDGARv4.2 at their peak in the 1990s, albeit significant underestimation for the other time periods. There are no bottom-up emission estimates for C8F18, thus the emission rates reported here are the first for C8F18. The reported inventories for C4F10, C5F12 and C6F14 to UNFCCC are five to ten times lower than those

  19. Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3

    PubMed Central

    Vogel, Georg F.; Klee, Katharina M.C.; Janecke, Andreas R.; Müller, Thomas

    2015-01-01

    Mutations in the motor protein Myosin Vb (Myo5B) or the soluble NSF attachment protein receptor Syntaxin 3 (Stx3) disturb epithelial polarity and cause microvillus inclusion disease (MVID), a lethal hereditary enteropathy affecting neonates. To understand the molecular mechanism of Myo5B and Stx3 interplay, we used genome editing to introduce a defined Myo5B patient mutation in a human epithelial cell line. Our results demonstrate a selective role of Myo5B and Stx3 for apical cargo exocytosis in polarized epithelial cells. Apical exocytosis of NHE3, CFTR (cystic fibrosis transmembrane conductance regulator), and GLUT5 required an interaction cascade of Rab11, Myo5B, Slp4a, Munc18-2, and Vamp7 with Stx3, which cooperate in the final steps of this selective apical traffic pathway. The brush border enzymes DPPIV and sucrase-isomaltase still correctly localize at the apical plasma membrane independent of this pathway. Hence, our work demonstrates how Myo5B, Stx3, Slp4a, Vamp7, Munc18-2, and Rab8/11 cooperate during selective apical cargo trafficking and exocytosis in epithelial cells and thereby provides further insight into MVID pathophysiology. PMID:26553929

  20. Kinetics of the transient optical absorption in Li2B4O7 and LiB3O5 lithium borate crystals

    NASA Astrophysics Data System (ADS)

    Ogorodnikov, I. N.; Kiseleva, M. S.

    2012-04-01

    This paper reports on a study of the kinetics of electron tunneling transport between electron and hole centers in Li2B4O7 and LiB3O5 lithium borate crystals under the conditions where the mobility of one of the partners in the recombination process is thermally stimulated. A mathematical model has been proposed to describe all specific features in the relaxation kinetics of the induced optical density observed in Li2B4O7 (LTB) and LiB3O5 (LBO) nonlinear optical crystals within a broad time interval of 10-8-1 s after a radiation pulse. The results of calculations have been compared with experimental data on transient optical absorption (TOA) of LTB and LBO crystals in the visible and ultraviolet spectral regions. The nature of the radiation defects responsible for TOA and the dependence of the TOA decay kinetics on temperature, excitation power, and other experimental conditions have been discussed.

  1. Deposition of calcium phosphate coatings using condensed phosphates (P2O7(4-) and P3O10(5-)) as phosphate source through induction heating.

    PubMed

    Zhou, Huan; Hou, Saisai; Zhang, Mingjie; Yang, Mengmeng; Deng, Linhong; Xiong, Xinbo; Ni, Xinye

    2016-12-01

    In present work condensed phosphates (P2O7(4-) and P3O10(5-)) were used as phosphate source in induction heating to deposit calcium phosphate coatings. The phase, morphology, and composition of different phosphate-related coatings were characterized and compared using XRD, FTIR, and SEM analyses. Results showed that P2O7(4-)formed calcium pyrophosphate hydrate coatings with interconnected cuboid-like particles. The as-deposited calcium tripolyphosphate hydrate coating with P3O10(5-) was mainly composed of flower-like particles assembled by plate-like crystals. The bioactivity and cytocompatibility of the coatings were also studied. Moreover, the feasibility of using hybrid phosphate sources for preparing and depositing coatings onto magnesium alloy was investigated. PMID:27612721

  2. The thermodynamic properties of 4,5,9,10-tetrahydropyrene and 1,2,3,6,7,8-hexahydropyrene. [Tetrahydropyrene and hexahydropyrene

    SciTech Connect

    Chirico, R.D.; Knipmeyer, S.E.; Nguyen, A.; Smith, N.K.; Steele, W.V.

    1992-12-01

    Measurements leading to the calculation of the ideal-gas thermodynamic properties are reported for 4,5,9,10-tetrahydropyrene and 1,2,3,6,7,8-hexahydropyrene. Experimental methods included combustion calorimetry, adiabatic heat-capacity calorimetry, vibrating-tube densitometry, comparative ebulliometry, inclined-piston gauge manometry, and differential-scanning calorimetry (d.s.c.). Critical properties were estimated for both materials based on the measurement results. Entropies, enthalpies, and Gibbs energies of formation were derived for the ideal gases for selected temperatures between 380 K and 700 K. The property-measurement results reported here for 4,5,9,10-tetrahydropyrene and 1,2,3,6,7,8-hexahydropyrene are the first for these important intermediates in the pyrene/H[sub 2] hydrogenation reaction network.

  3. Solid state structure of new 5-[2-(N,N-diethylamino)ethoxy]-4,7-dimethylcoumarins by X-ray and 13C CP/MAS NMR

    NASA Astrophysics Data System (ADS)

    Ostrowska, Kinga; Hejchman, Elżbieta; Dobrzycki, Łukasz; Maciejewska, Dorota

    2015-05-01

    5-[2-(N,N-diethylamino)ethoxy]-4,7-dimethylcoumarin (1) and 6-acetyl-5-[2-(N,N-diethylamino)ethoxy]-4,7-dimethylcoumarin (2) were synthesized in a traditional way and microwave-assisted synthesis. Crystals of 2 in form of hydrochloride salt (3) were investigated using single crystal X-ray diffraction technique. In the crystal lattice of 3 there is only one type of strong hydrogen bond present involving protonated amino group and chloride anion. The whole structure is dominated by weak C-H…Cl-, C-H…O contacts. There is also visible aggregation of cations in stacks due to π-π interactions. The solid state structures of 1 and 2 derived from 13C CP/MAS NMR spectra were also proposed.

  4. Measurements of exclusive photoproduction processes at large values of t and u from 4 to 7.5 GeV

    USGS Publications Warehouse

    Anderson, R.L.; Gustavson, D.B.; Ritson, D.M.; Weitsch, G.A.; Halpern, H.J.; Prepost, R.; Tompkins, D.H.; Wiser, D.E.

    1976-01-01

    Exclusive photoproduction cross sections have been measured for the processes p+n, p0p, p-++, p0p, pK+, and pK+0 at large t and u values at several energies for each process between 4 and 7.5 GeV. These measurements taken together with past data taken at small values of t and u provide complete angular distributions. The data show the usual small t and u peaks and a central region in which the cross section decreases approximately as s-7. The results are discussed within the context of parton or constituent models. ?? 1976 The American Physical Society.

  5. Synthesis, anticancer activity, and SAR analyses of compounds containing the 5:7-fused 4,6,8-triaminoimidazo[4,5-e][1,3]diazepine ring system.

    PubMed

    Xie, Min; Lapidus, Rena G; Sadowska, Mariola; Edelman, Martin J; Hosmane, Ramachandra S

    2016-06-15

    Described herein are our limited structure-activity relationship (SAR) studies on a 5:7-fused heterocycle (1), containing the 4,6,8-triaminoimidazo[4,5-e][1,3]diazepine ring system, whose synthesis and potent broad-spectrum anticancer activity we reported a few years ago. Our SAR efforts in this study are mainly focused on judicial attachment of substituents at N-1 and N(6)-positions of the heterocyclic ring. Our results suggest that there is some subtle correlation between the substituents attached at the N-1 position and those attached at the N(6)-position of the heterocycle. It is likely that there is a common hydrophobic binding pocket on the target protein that is occupied by the substituents attached at the N-1 and N(6)-positions of the heterocyclic ligand. This pocket appears to be large enough to hold either a C-18 alkyl chain of N(6) and no attachment at N-1, or a combined C-10 at N(6) and a CH2Ph at N-1. Any alkyl chain shorter or longer than C-10 at N(6) with a CH2Ph attached at N-1, would result in decrease of biological activity. PMID:27134120

  6. Cytochrome P450-Mediated Metabolism and DNA Binding of 2-Amino-1,7-dimethylimidazo[4,5-g]quinoxaline and its Carcinogenic Isomer 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline in Mice

    PubMed Central

    Turesky, Robert J.; Bessette, Erin E.; Dunbar, Deborah; Liberman, Rosa G.; Skipper, Paul L.

    2012-01-01

    2-Amino-1,7-dimethylimidazo[4,5-g]quinoxaline (MeIgQx) is a recently discovered heterocyclic aromatic amine (HAA) that is formed during the cooking of meats. MeIgQx is an isomer of 2-amino-3,8-dimethylmidazo[4,5-f]quinoxaline (MeIQx), a rodent carcinogen and possible human carcinogen that also occurs in cooked meats. MeIgQx is a bacterial mutagen but knowledge about its metabolism and carcinogenic potential is lacking. Metabolism studies on MeIgQx and MeIQx were conducted with human and mouse liver microsomes, and recombinant human P450s. DNA binding studies were also investigated in mice to ascertain the genotoxic potential of MeIgQx in comparison to MeIQx. Both HAAs underwent comparable rates of N-oxidation to form genotoxic N-hydroxylated metabolites with mouse liver microsomes (0.2 - 0.3 nmol/min/mg protein). The rate of N-oxidation of MeIQx was 4-fold greater than the rate of N-oxidation of MeIgQx with human liver microsomes (1.7 vs 0.4 nmol/min/mg protein). The rate of N-oxidation, by recombinant human P450 1A2, was comparable for both substrates (6 pmol/min/pmol P450 1A2). MeIgQx also underwent N-oxidation by human P450s 1A1 and 1B1 at appreciable rates, whereas MeIQx was poorly metabolized by these P450s. The potential of MeIgQx and MeIQx to form DNA adducts was assessed in female C57BL/6 mice given [14C]-MeIgQx (10 μCi, 9.68 mg/kg body wt) or [14C]-MeIQx (10 μCi, 2.13 mg/kg body wt). DNA adduct formation in liver, pancreas and colorectum was measured by accelerator mass spectrometry at 4, 24 or 48 h post-treatment. Variable levels of adducts were detected in all organs. The adduct levels were similar for both HAAs, when adjusted for dose, and ranged from 1 to 600 adducts per 107 nucleotides per mg/kg dose. Thus, MeIgQx undergoes metabolic activation and binds to DNA at levels that are comparable to MeIQx. Given the high amounts of MeIgQx formed in cooked meats, further investigations are warranted to assess the carcinogenic potential of this HAA. PMID

  7. Global emission estimates and radiative impact of C4F10, C5F12, C6F14, C7F16 and C8F18

    NASA Astrophysics Data System (ADS)

    Ivy, D. J.; Rigby, M.; Baasandorj, M.; Burkholder, J. B.; Prinn, R. G.

    2012-05-01

    Global emission estimates based on new atmospheric observations are presented for the acylic high molecular weight perfluorocarbons (PFCs): decafluorobutane (C4F10), dodecafluoropentane (C5F12), tetradecafluorohexane (C6F14), hexadecafluoroheptane (C7F16) and octadecafluorooctane (C8F18). Emissions are estimated using a 3-dimensional chemical transport model and an inverse method that includes a growth constraint on emissions. The observations used in the inversion are based on newly measured archived air samples that cover a 39-yr period, from 1973 to 2011, and include 36 Northern Hemispheric and 46 Southern Hemispheric samples (Ivy et al., 2012). The derived emission estimates show that global emission rates were largest in the 1980s and 1990s for C4F10 and C5F12, and in the 1990s for C6F14,C7F16 and C8F18. After a subsequent decline, emissions have remained relatively stable, within 20%, for the last 5 yr. Bottom-up emission estimates are available from the Emission Database for Global Atmospheric Research version 4.2 (EDGARv4.2) for C4F10, C5F12, C6F14 and C7F16, and inventories of C4F10, C5F12 andC6F14 are reported to the United Nations' Framework Convention on Climate Change (UNFCCC) by Annex 1 countries that have ratified the Kyoto Protocol. The atmospheric measurement based emission estimates are 20 times larger than EDGARv4.2 for C4F10 and over three orders of magnitude for C5F12. The derived emission estimates for C6F14 largely agree with the bottom-up estimates from EDGARv4.2. Moreover, the C7F16 emission estimates are comparable to those of EDGARv4.2 at their peak in the 1990s, albeit significant underestimation for the other time periods. There are no bottom-up emission estimates for C8F18, thus the emission rates reported here are the first for C8F18. The reported inventories for C4F10, C5F12 and C6F14 to UNFCCC are five to ten times lower than those estimated in this study. In addition, we present measured infrared absorption spectra for C7F16 and C8

  8. 3-Methyl-4-(2-phenyl-1,2,4-triazolo[1,5-a]pyrimidin-7-yl)furazan.

    PubMed

    Suponitsky, Kyrill Yu; Chernyshev, Victor M; Palysaeva, Nadezhda V; Sheremetev, Aleksei B

    2013-10-16

    In the title mol-ecule, C14H10N6O, the planes of the methyl-furazan fragment and the phenyl ring attached to the triazolo-pyrimidine bicycle are twisted from the mean plane of the bicycle at angles of 45.92 (5) and 5.45 (4)°, respectively. In the crystal, π-π inter-actions, indicated by short distances [in the range 3.456 (3)-3.591 (3) Å] between the centroids of the five- and six-membered rings of neighbouring mol-ecules, link the mol-ecules into stacks propagating along the c-axis direction. PMID:24454090

  9. 3-Methyl-4-(2-phenyl-1,2,4-triazolo[1,5-a]pyrimidin-7-yl)furazan

    PubMed Central

    Suponitsky, Kyrill Yu.; Chernyshev, Victor M.; Palysaeva, Nadezhda V.; Sheremetev, Aleksei B.

    2013-01-01

    In the title mol­ecule, C14H10N6O, the planes of the methyl­furazan fragment and the phenyl ring attached to the triazolo­pyrimidine bicycle are twisted from the mean plane of the bicycle at angles of 45.92 (5) and 5.45 (4)°, respectively. In the crystal, π–π inter­actions, indicated by short distances [in the range 3.456 (3)–3.591 (3) Å] between the centroids of the five- and six-membered rings of neighbouring mol­ecules, link the mol­ecules into stacks propagating along the c-axis direction. PMID:24454090

  10. 3,5-Dibenzoyl-4-(3-phenoxyphenyl)-1,4-dihydro-2,6-dimethylpyridine (DP7) as a new multidrug resistance reverting agent devoid of effects on vascular smooth muscle contractility.

    PubMed

    Saponara, Simona; Kawase, Masami; Shah, Anamik; Motohashi, Noboru; Molnar, Joseph; Ugocsai, Katalin; Sgaragli, Giampietro; Fusi, Fabio

    2004-02-01

    The aim of this study was to investigate the effects of 3,5-diacetyl- (DP1-DP5) and 3,5-dibenzoyl-1,4-dihydropyridines (DP6-DP11) on vascular functions in vitro, by comparing their mechanical and electrophysiological actions in rat aorta rings and single rat tail artery myocytes, respectively, and to quantify their multidrug resistance (MDR)-reversing activity in L5178 Y mouse T-lymphoma cells transfected with MDR1 gene. In rat aorta, the 11 compounds tested, but 3,5-dibenzoyl-4-(3-phenoxyphenyl)-1,4-dihydro-2,6-dimethylpyridine (DP7), 3,5-dibenzoyl-4-(3-chlorophenyl)-1,4-dihydro-2,6-dimethylpyridine (DP9), 3,5-dibenzoyl-4-(4-chlorophenyl)-1,4-dihydro-2,6-dimethylpyridine (DP10) and 3,5-dibenzoyl-4-phenyl-1,4-dihydro-2,6-dimethylpyridine (DP11), antagonized 60 mm K+ (K60)-induced contraction in a concentration-dependent manner, with IC50 (m) values ranging between 5.65 x 10(-7) and 2.23 x 10(-5). The 11 dihydropyridines tested, but DP7, inhibited L-type Ca2+ current recorded in artery myocytes in a concentration-dependent manner, with IC50 (M) values ranging between 1.12 x 10(-6) and 6.90 x 10(-5). The K+ -channel opener cromakalim inhibited the Ca2+ -induced contraction in K30 but not that evoked in K60. On the contrary, DP7 was ineffective in both experimental conditions. When the rings were preincubated with 1 mm Ni2+ plus 1 microm nifedipine, the response to phenylephrine was significantly reduced by 2,5-di-t-butyl-1,4-benzohydroquinone (BHQ), a well-known endoplasmic reticulum Ca2+ -ATPase inhibitor. DP7 had no effects on this model system. In L5178 MDR cell line, the 11 dihydropyridines tested, but 3,5-diacetyl-4-(2-nitrophenyl)-1,4-dihydro-2,6-dimethylpyridine (DP1), 3,5-diacetyl-4-(3-phenoxyphenyl)-1,4-dihydro-2,6-dimethylpyridine (DP2) and 3,5-diacetyl-4-(3-chlorophenyl)-1,4-dihydro-2,6-dimethylpyridine (DP4), exhibited an MDR-reversing activity, with IC50 values ranging between 3.02 x 10(-7) and 4.27 x 10(-5), DP7 being the most potent. In conclusion, DP7

  11. 7 CFR 1781.5 - Eligibility.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 12 2014-01-01 2013-01-01 true Eligibility. 1781.5 Section 1781.5 Agriculture... construction facilities. (6) Health and sanitation standards, water pollution control, and environmental... loans. (3) Land use zoning. (4) Acquiring necessary property, lands, and rights. (5) Obtaining...

  12. 7 CFR 274.5 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false 274.5 Section 274.5 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM ISSUANCE AND USE OF COUPONS § 274.5...

  13. Vasorelaxant and antiplatelet activity of 4,7-dimethyl-1,2,5-oxadiazolo[3,4-d]pyridazine 1,5,6-trioxide: role of soluble guanylate cyclase, nitric oxide and thiols

    PubMed Central

    Kots, Alexander Ya; Grafov, Mikhail A; Khropov, Yuri V; Betin, Vasily L; Belushkina, Natalya N; Busygina, Olga G; Yazykova, Marina Yu; Ovchinnikov, Igor V; Kulikov, Alexander S; Makhova, Nina N; Medvedeva, Natalya A; Bulargina, Tamara V; Severina, Irina S

    2000-01-01

    Certain heterocyclic N-oxides are vasodilators and inhibitors of platelet aggregation. The pharmacological activity of the furoxan derivative condensed with pyridazine di-N-oxide 4,7-dimethyl-1,2,5-oxadiazolo[3,4-d]pyridazine 1,5,6-trioxide (FPTO) and the corresponding furazan (FPDO) was studied. FPTO reacted with thiols generating nitrite (NO), S-nitrosoglutathione and hydroxylamine (nitroxyl) and converted oxyHb to metHb. FPDO did not generate detectable amounts of NO-like species but reacted with thiols and oxyHb. FPTO and FPDO haem-dependently stimulated the activity of soluble guanylate cyclase (sGC) and this stimulation was inhibited by 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) and by 0.1 mM dithiothreitol. FPTO relaxed noradrenaline-precontracted aortic rings and its concentration-response curve was biphasic (pIC50=9.03±0.13 and 5.85±0.06). FPDO was significantly less potent vasodilator (pIC50=5.19±0.14). The vasorelaxant activity of FPTO and FPDO was inhibited by ODQ. oxyHb significantly inhibited only FPTO-dependent relaxation. FPTO and FPDO were equipotent inhibitors of ADP-induced platelet aggregation (IC50=0.63±0.15 and 0.49±0.05 μM, respectively). The antiplatelet activity of FPTO (but not FPDO) was partially suppressed by oxyHb. The antiaggregatory effects of FPTO and FPDO were only partially blocked by sGC inhibitors. FPTO and FPDO (10–20 μM) significantly increased cyclic GMP levels in aortic rings and platelets and this increase was blocked by ODQ. Thus, FPTO can generate NO and, like FPDO, reacts with thiols and haem. The vasorelaxant activity of FPTO and FPDO is sGC-dependent and a predominant role is played by NO at FPTO concentrations below 1 μM. On the contrary, inhibition of platelet aggregation is only partially related to sGC activation. PMID:10725265

  14. Yb{sub 5}Ni{sub 4}Sn{sub 10} and Yb{sub 7}Ni{sub 4}Sn{sub 13}: New polar intermetallics with 3D framework structures

    SciTech Connect

    Lei Xiaowu; Sun Zhongming; Li Longhua; Zhong Guohua; Hu Chunli; Mao Jianggao

    2010-04-15

    The title compounds have been obtained by solid state reactions of the corresponding pure elements at high temperature, and structurally characterized by single-crystal X-ray diffraction studies. Yb{sub 5}Ni{sub 4}Sn{sub 10} adopts the Sc{sub 5}Co{sub 4}Si{sub 10} structure type and crystallizes in the tetragonal space group P4/mbm (No. 127) with cell parameters of a=13.785(4) A, c=4.492 (2) A, V=853.7(5) A{sup 3}, and Z=2. Yb{sub 7}Ni{sub 4}Sn{sub 13} is isostructural with Yb{sub 7}Co{sub 4}InGe{sub 12} and crystallizes in the tetragonal space group P4/m (No. 83) with cell parameters of a=11.1429(6) A, c=4.5318(4) A, V=562.69(7) A{sup 3}, and Z=1. Both structures feature three-dimensional (3D) frameworks based on three different types of one-dimensional (1D) channels, which are occupied by the Yb atoms. Electronic structure calculations based on density functional theory (DFT) indicate that both compounds are metallic. These results are in agreement with those from temperature-dependent resistivity and magnetic susceptibility measurements. - Graphical abstract: Two new ytterbium nickel stannides, namely, Yb{sub 5}Ni{sub 4}Sn{sub 10} and Yb{sub 7}Ni{sub 4}Sn{sub 13}, have been synthesized and structurally characterized by single-crystal X-ray diffraction studies. Both their structures feature three-dimensional (3D) frameworks based on three different types of one-dimensional (1D) channels, which are situated by all the Yb atoms. Electronic structure calculations based on density functional theory (DFT) indicate that both compounds are metallic, which are in accordance with the results from temperature-dependent resistivity and magnetic susceptibility measurements.

  15. 4-[(3-Benzamido­methyl-6-phenyl-6,7-dihydro-5H-1,2,4-triazolo[3,4-b][1,3,4]thia­diazin-7-yl)carbon­yl]-3-phenyl-1,2,3-oxadiazol-3-ium-5-olate 0.06-hydrate

    PubMed Central

    Fun, Hoong-Kun; Hemamalini, Madhukar; Nithinchandra; Kalluraya, Balakrishna

    2011-01-01

    The asymmetric unit of the title compound, C27H21N7O4S·0.06H2O, contains four syndone mol­ecules and a water mol­ecule with a site occupancy of 0.25. In two of the syndone mol­ecules, three atoms in a terminal phenyl ring are disordered over two sets of sites, with occupancy ratios of 0.500 (18):0.500 (18) and 0.512 (17):0.488 (17). The dihedral angles between terminal phenyl rings for the syndone mol­ecules are 23.3 (4), 45.57 (16), 68.46 (16) and 56.5 (3)°. In the crystal, mol­ecules are connected via N—H⋯N, N—H⋯O, O—H⋯O, O—H⋯N and C—H⋯O hydrogen bonds, forming a three-dimensional network. PMID:21522639

  16. Theoretical vibrations of carbon chains C3, C4, C5, C6, C7, C8, and C9

    NASA Technical Reports Server (NTRS)

    Kurtz, Joe; Adamowicz, Ludwik

    1991-01-01

    The MBPT (2) procedure with the 6-31g (asterisk) basis set was used to study nearly linear carbon chains. The theoretical vibrational frequencies of the molecules C3 through C9 are presented and, for C3 through C6, compared to experimental stretching frequencies and their (C-13)/(C-12) isotopomers. Predictions for C7, C8, and C9 stretching frequencies are calculated by directly scaling the theoretical frequencies with factors derived from experimental-to-theoretical ratios known for the smaller molecules.

  17. 4,5-Dibromo-2,7-di-tert-butyl-9,9-dimethyl-9H-thioxanthene

    PubMed Central

    Rubio, Omayra H.; Fuentes de Arriba, Angel L.; Sanz, Francisca; Muniz, Francisco M.; Morán, Joaquín R.

    2012-01-01

    In the title compound, C23H28Br2S, the thioxanthene unit is twisted, showing a dihedral angle of 29.3 (5)° between the benzene rings. When projected along [001], the packing shows two types of channels. The crystal studied was a racemic twin. PMID:22719586

  18. Substituted 5,6-(Dihydropyrido[3,4-d]pyrimidin-7(8H)-yl)-methanones as P2X7 Antagonists.

    PubMed

    Ziff, Jeannie; Rudolph, Dale A; Stenne, Brice; Koudriakova, Tatiana; Lord, Brian; Bonaventure, Pascal; Lovenberg, Timothy W; Carruthers, Nicholas I; Bhattacharya, Anindya; Letavic, Michael A; Shireman, Brock T

    2016-04-20

    We describe the synthesis of a novel class of brain penetrating P2X7 antagonists with high potency at both the rat and human P2X7 receptors. Disclosed herein are druglike molecules with demonstrated target engagement of the rat P2X7 receptors after an oral dose. Specifically, compound 20 occupied the P2X7 receptors >80% over the 6 h time course as measured by an ex vivo radioligand binding experiment. In a dose-response assay, this molecule has a plasma EC50 of 8 ng/mL. Overall, 20 has suitable druglike properties and pharmacokinetics in rat and dog. This molecule and others disclosed herein will serve as additional tools to elucidate the role of the P2X7 receptor in neuropsychiatric disorders. PMID:26754558

  19. Paroxysmal extreme pain disorder mutations within the D3/S4–S5 linker of Nav1.7 cause moderate destabilization of fast inactivation

    PubMed Central

    Jarecki, Brian W; Sheets, Patrick L; Jackson II, James O; Cummins, Theodore R

    2008-01-01

    Single-point missense mutations in the peripheral neuronal voltage-gated sodium channel Nav1.7 are implicated in the painful inherited neuropathy paroxysmal extreme pain disorder (PEPD). The Nav1.7 PEPD mutations are located in regions of the channel suggested to play important roles in fast inactivation. PEPD mutations in the putative inactivation gate have been reported to significantly impair fast inactivation, resulting in pronounced persistent currents. However, PEPD mutations in the S4–S5 linker of domain 3 (D3/S4–S5) had not been characterized and the roles of specific residues in this linker in channel gating are unclear. We functionally characterized two of the D3/S4–S5 PEPD mutations (V1298F and V1299F) and compared their effects on gating to an adjacent non-PEPD mutation (V1300F) and the I1461T PEPD mutation, located in the putative inactivation gate. The primary effect of the V1298F and V1299F mutations is to shift the voltage dependence of fast inactivation by ∼20 mV in the depolarizing direction. We observed a similar effect with the PEPD mutation I1461T. Interestingly, while all three PEPD mutations increased persistent currents, the relative amplitudes (∼6% of peak) were much smaller than previously reported for the I1461T mutation. In contrast, the main effect of the V1300F mutation was a depolarizing shift in the voltage dependence of activation. These data demonstrate that (1) mutations within D3/S4–S5 affect inactivation of Nav1.7 in a residue-specific manner and (2) disruption of the fast-inactivated state by PEPD mutations can be more moderate than previously indicated, which has important implications for the pathophysiology of PEPD. PMID:18599537

  20. Engineering substrate specificity of succinic semialdehyde reductase (AKR7A5) for efficient conversion of levulinic acid to 4-hydroxyvaleric acid.

    PubMed

    Yeon, Young Joo; Park, Hyung-Yeon; Yoo, Young Je

    2015-09-20

    Engineering enzyme substrate specificity is a promising approach that can expand the applicability of enzymes for the biocatalytic production of industrial chemicals and fuels. In this study, succinic semialdehyde reductase (AKR7A5) was engineered for the conversion of levulinic acid to 4-hydroxyvaleric acid. Levulinic acid is a derivative of cellulosic biomass, and 4-hydroxyvaleric acid is a potential precursor to bio-polymers and fuels. Therefore, the enzymatic conversion of levulinic acid to 4-hydroxyvaleric acid is of special significance in that this conversion could provide a meaningful basis for the bio-production of useful chemicals from cellulosic biomass. In engineering the substrate specificity of the AKR7A5, a rational design approach with the aid of enzyme-substrate interatomic contact analysis was applied. The Met13 residue was selected as a key mutation site, and substitutions of the residue with six hydrophobic amino acids were applied. As a result, four mutants with enhanced catalytic activity toward levulinic acid were obtained, and the most improved mutant, Met13Trp, exhibited a 7.0-fold increase in catalytic efficiency. Additionally, the structural effects of the positive mutations were investigated to analyze the structural basis for the enzyme substrate specificity with the target substrate. PMID:26113216

  1. Oxidation of U(IV) by atmospheric oxygen in pH 1.5-7.4 aqueous solutions

    SciTech Connect

    Shilov, V P; Yusov, A B; Peretrukhin, V F; Delegard, Calvin H; Gogolev, A V; Fedosseev, A M; Kazansky, L P

    2007-10-11

    U(IV) oxidation by oxygen in weakly acidic and neutral solutions at pH 1.5-5 has been found to proceed by a pseudo-first order reaction with an induction period dependent on the accumulation of U(VI). The presence or the addition of U(VI) accelerates U(IV) oxidation. The rate constants of U(IV) oxidation by air have been determined. The oxidation mechanism involves the interaction of hydrolyzed U(OH)22+ species with O2, HO2, H2O2, and OH•, the formation of U(V), and the reaction of U(V) with the listed oxidants. After the accumulation of sufficient U(VI), the reaction U(VI) + U(IV) ⇌2 U(V) becomes rate-controlling. The formation of colloidal polymer solution has been observed at pH 1.5 to 5 during the oxidation of U(IV) by air, during photochemical reduction of U(VI), or by mixing of U(VI) and U(IV) solutions. The colloidal polymer solution has been found to be stable at 20-100°C under anaerobic conditions. The polymer was isolated from the solution by centrifugation at 8000 G. Chemical and X-ray photoelectron spectroscopy studies have shown the polymer to be a sparingly soluble mixed U(VI)-U(IV) hydroxide.

  2. Environmentally relevant concentrations of arsenite and monomethylarsonous acid inhibit IL-7/STAT5 cytokine signaling pathways in mouse CD3+CD4-CD8- double negative thymus cells.

    PubMed

    Xu, Huan; Lauer, Fredine T; Liu, Ke Jian; Hudson, Laurie G; Burchiel, Scott W

    2016-04-15

    Environmental arsenic exposure is a public health issue. Immunotoxicity induced by arsenic has been reported in humans and animal models. The purpose of this study was to evaluate mechanisms of As(+3) and MMA(+3) toxicity in mouse thymus cells. Because we know that MMA(+3) inhibits IL-7 signaling in mouse bone marrow pre-B cells, we studied the influence of As(+3) and MMA(+3) on T cell development in the thymus at the earliest stage of T cell development (CD4-CD8-, double negative, DN) which requires IL-7 dependent signaling. We found in a DN thymus cell line (D1) that a low concentration of MMA(+3) (50 nM) suppressed IL-7 dependent JAK1, 3 and STAT5 signaling. As(+3) suppressed STAT5 and JAK3 at higher concentrations (500 nM). Cell surface expression of the IL-7 receptor (CD127) was also suppressed by 50 nM MMA(+)3, but was increased by 500 NM As(+3), indicating possible differences in the mechanisms of action of these agents. A decrease in cyclin D1 protein expression was observed in D1 cells exposed to As(+3) at 500 nM and MMA(+3) starting at 50 nM, suggesting that arsenic at these environmentally-relevant doses suppresses early T cell development through the inhibition of IL-7 signaling pathway. PMID:26921788

  3. Synthesis and structure of a novel mesomeric betaine 6,7-dimethyl-2 H-pyrazolo[4,3- e]tetrazolo[4,5- b][1,2,4]triazine

    NASA Astrophysics Data System (ADS)

    Karczmarzyk, Zbigniew; Mojzych, Mariusz; Rykowski, Andrzej

    2007-03-01

    The synthesis and structural characterization by 1H NMR, HRMS, elemental analysis and X-ray crystallography of a novel mesomeric betaine, 6,7-dimethyl-2 H-pyrazolo[4,3- e]tetrazolo[4,5- b][1,2,4]triazine 8a are described. The compound 8a crystallizes in the monoclinic system in the space group P2 1/n with a = 11.766(2), b = 5.8097(12), c = 12.389(3) Å, β = 107.14(3)°, V = 809.2(3) Å 3, Z = 4, Dcalc = 1.561 gcm -3, λ(Cu Kα) = 1.54178 Å, μ = 0.953 mm -1 and final R = 0.0378 for 1526 reflections. The molecule as a whole has an almost planar conformation and the bond lengths and angles have the values characteristic for π-electron system. The molecular packing in the crystal is influenced by the van der Waals forces and presence of weak π-π interactions. The electronic properties (net charge and MEP distributions and dipole moment) of the molecule 8a are calculated using AM1 approximation. Twelve dipolar canonical forms for molecule 8a are presented and their contribution in mesomeric betaine structure of 8a is discussed (molecular mechanics calculations).

  4. Sr2OsO5 and Sr7Os4O19, Two Structurally Related, Mott Insulating Osmates(VI) Exhibiting Substantially Reduced Spin Paramagnetic Response.

    PubMed

    Mohitkar, Shrikant A; Schnelle, Walter; Felser, Claudia; Jansen, Martin

    2016-08-15

    The new osmates(VI), Sr2OsO5 and Sr7Os4O19, feature quasi-1-D polyoxo anions, consisting of corner sharing [OsO6] octahedra. In both compounds, the magnetic moment at T = 300 K is significantly lower (1.2-1.3 μB/Os-atom) than the value expected for S = 1. For neither of the new osmates(VI) is any evidence for long-range magnetic order found. For Sr7Os4O19, magnetic susceptibility suggests an antiferromagnetic ordering at TN = 43(3) K; however, no corresponding anomaly is visible in specific heat. Both compounds are semiconductors. PMID:27479609

  5. CTBT (7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine) producing ROS affects growth and viability of filamentous fungi.

    PubMed

    Culakova, Hana; Dzugasova, Vladimira; Gbelska, Yvetta; Subik, Julius

    2012-03-01

    CTBT (7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine) causes intracellular superoxide production and oxidative stress and enhances the susceptibility of Saccharomyces cerevisiae, Candida albicans, and C. glabrata cells to cycloheximide, 5-fluorocytosine, and azole antimycotic drugs. Here, we demonstrate the antifungal activity of CTBT against 14 tested filamentous fungi. CTBT prevented spore germination and mycelial proliferation of Aspergillus niger and the pathogenic Aspergillus fumigatus. The action of CTBT is fungicidal. CTBT increased the formation of reactive oxygen species in fungal mycelium as detected by 2',7'-dichlorodihydrofluorescein diacetate and reduced the radial growth of colonies in a dose-dependent manner. Co-application of CTBT and itraconazole led to complete inhibition of fungal growth at dosages lower than the chemicals alone. Antifungal and chemosensitizing activities of CTBT in filamentous fungi may be useful in combination treatments of infections caused by drug-resistant fungal pathogens. PMID:22212016

  6. Evaluation of the Replication, Pathogenicity, and Immunogenicity of Avian Paramyxovirus (APMV) Serotypes 2, 3, 4, 5, 7, and 9 in Rhesus Macaques

    PubMed Central

    Khattar, Sunil K.; Nayak, Baibaswata; Kim, Shin-Hee; Xiao, Sa; Samal, Sweety; Paldurai, Anandan; Buchholz, Ursula J.; Collins, Peter L.; Samal, Siba K.

    2013-01-01

    Avian paramyxoviruses (APMV) serotypes 1–9 are frequently isolated from domestic and wild birds worldwide. APMV-1 (also called Newcastle disease virus, NDV) is attenuated in non-human primates and is being developed as a candidate human vaccine vector. The vector potential of the other serotypes was unknown. In the present study, we evaluated nine different biologically- or recombinantly-derived APMV strains for the ability to replicate and cause disease in rhesus macaque model. Five of the viruses were: biologically-derived wild type (wt) APMV-2, -3, -5, -7 and -9. Another virus was a recombinant (r) version of wt APMV-4. The remaining three viruses were versions of wt rAPMV-2, -4 and -7 in which the F cleavage site had been modified to be multi-basic. Rhesus macaques were inoculated intranasally and intratracheally and monitored for clinical disease, virus shedding from the upper and lower respiratory tract, and seroconversion. Virus shedding was not detected for wt APMV-5. Very limited shedding was detected for wt rAPMV-4 and modified rAPMV-4, and only in a subset of animals. Shedding by the other viruses was detected in every infected animal, and usually from both the upper and lower respiratory tract. In particular, shedding over a number of days in every animal was observed for modified rAPMV-2, wt APMV-7, and modified rAPMV-7. Modification of the F protein cleavage site appeared to increase shedding by wt rAPMV-2 and marginally by wt rAPMV-4. All APMVs except wt APMV-5 induced a virus-specific serum antibody response in all infected animals. None of the animals exhibited any clinical disease signs. These results indicate that APMVs 2, 3, 4, 7, and 9 are competent to infect non-human primates, but are moderately-to-highly restricted, depending on the serotype. This suggests that they are not likely to significantly infect primates in nature, and represent promising attenuated candidates for vector development. PMID:24130713

  7. Antileishmanial Effect of 5,3′-Hydroxy-7,4′-dimethoxyflavanone of Picramnia gracilis Tul. (Picramniaceae) Fruit: In Vitro and In Vivo Studies

    PubMed Central

    Robledo, Sara M.; Cardona, Wilson; Ligardo, Karen; Henao, Jéssica; Arbeláez, Natalia; Montoya, Andrés; Alzate, Fernando; Pérez, Juan M.; Arango, Victor; Vélez, Iván D.; Sáez, Jairo

    2015-01-01

    Species of Picramnia genus are used in folk medicine to treat or prevent skin disorders, but only few species have been studied for biological activity and chemical composition. P. gracilis Tul. is a native species from Central and South America and although its fruits are edible, phytochemical analysis or medicinal uses of this species are not known. In the search of candidates to antileishmanial drugs, this work aimed to evaluate the antileishmanial activity of P. gracilis Tul. in in vitro and in vivo studies. Only ethanolic extract of fruits showed leishmanicidal activity. The majoritarian metabolite was 5,3′-hydroxy-7,4′-dimethoxyflavanone ether that exhibited high activity against L. (V.) panamensis (EC50 17.0 + 2.8 mg/mL, 53.7 μM) and low toxicity on mammalian U-937 cells, with an index of selectivity >11.8. In vivo studies showed that the flavanone administered in solution (2 mg/kg/day) or cream (2%) induces clinical improvement and no toxicity in hamsters with CL. In conclusion, this is the first report about isolation of 5,3′-hydroxy-7,4′-dimethoxyflavanone of P. gracilis Tul. The leishmanicidal activity attributed to this flavanone is also reported for the first time. Finally, the in vitro and in vivo leishmanicidal activity reported here for 5,3′-hydroxy-7,4′-dimethoxyflavanone offers a greater prospect towards antileishmanial drug discovery and development. PMID:26064104

  8. Spironolactone and its main metabolite canrenoic acid block hKv1.5, Kv4.3 and Kv7.1+minK channels

    PubMed Central

    Gómez, Ricardo; Núñez, Lucía; Caballero, Ricardo; Vaquero, Miguel; Tamargo, Juan; Delpón, Eva

    2005-01-01

    Both spironolactone (SP) and its main metabolite, canrenoic acid (CA), prolong cardiac action potential duration and decrease the Kv11.1 (HERG) current. We examined the effects of SP and CA on cardiac hKv1.5, Kv4.3 and Kv7.1+minK channels that generate the human IKur, Ito1 and IKs, which contribute to the control of human cardiac action potential duration. hKv1.5 currents were recorded in stably transfected mouse fibroblasts and Kv4.3 and Kv7.1+minK in transiently transfected Chinese hamster ovary cells using the whole-cell patch clamp. SP (1 μM) and CA (1 nM) inhibited hKv1.5 currents by 23.2±3.2 and 18.9±2.7%, respectively, shifted the midpoint of the activation curve to more negative potentials and delayed the time course of tail deactivation. SP (1 μM) and CA (1 nM) inhibited the total charge crossing the membrane through Kv4.3 channels at +50 mV by 27.1±6.4 and 27.4±5.7%, respectively, and accelerated the time course of current decay. CA, but not SP, shifted the inactivation curve to more hyperpolarised potentials (Vh−37.0±1.8 vs −40.8±1.6 mV, n=10, P<0.05). SP (10 μM) and CA (1 nM) also inhibited Kv7.1+minK currents by 38.6±2.3 and 22.1±1.4%, respectively, without modifying the voltage dependence of channel activation. SP, but not CA, slowed the time course of tail current decay. CA (1 nM) inhibited the IKur (29.2±5.5%) and the Ito1 (16.1±3.9%) recorded in mouse ventricular myocytes and the IK (21.8±6.9%) recorded in guinea-pig ventricular myocytes. A mathematical model of human atrial action potentials demonstrated that K+ blocking effects of CA resulted in a lengthening of action potential duration, both in normal and atrial fibrillation simulated conditions. The results demonstrated that both SP and CA directly block hKv1.5, Kv4.3 and Kv7.1+minK channels, CA being more potent for these effects. Since peak free plasma concentrations of CA ranged between 3 and 16 nM, these results indicated that blockade of these human

  9. Agonist-like activity of antibodies directed against the second extracellular loop of the human cardiac serotonin 5-HT4(e) receptor in transfected COS-7 cells.

    PubMed

    Bozon, V; Di Scala, E; Eftekhari, P; Hoebeke, J; Lezoualc'h, F; Fischmeister, R; Argibay, J

    2002-01-01

    We have previously reported that antipeptide antibodies directed against the second extracellular loop of the cardiac h5-HT4 receptor could block the activation of the L-type Ca channel in human atrial cardiomyocytes. In this paper we investigate the immunological and physiological activity of these antibodies, in a cell system expressing a larger amount of receptors than the atrial cells. The recombinant receptor was expressed at the surface of COS-7 cells under an active form (serotonin, EC50 = 1.81 x 10(-7) M), at a high level (375 +/- 25 fmol receptor/mg total protein) and was able to bind a specific ligand (GR113808) with a high affinity (Kd = 0.28 +/- 0.05 nM). In this system, the same anti-peptide antibodies used for the cardiac cells induced an "agonist-like" effect on the recombinant h5-HT4 receptor. These results are in line with those shown for others G-protein coupled receptors, as adrenoreceptors. In addition, this work showed that the effect of the antibodies is not only dependent on the epitopic region recognised but also on the molecular density and/or the cellular environment of the target receptors. Finally, our results support the hypothesis that the h5-HT4 receptor could be a new target for autoantibodies in patients with atrial arrhythmia. PMID:12448792

  10. The Inhibitory Mechanisms Study of 5,6,4'-Trihydroxy-7,3'-Dimethoxyflavone against the LPS-Induced Macrophage Inflammatory Responses through the Antioxidant Ability.

    PubMed

    Wang, Shih-Hao; Liang, Chia-Hua; Liang, Fong-Pin; Ding, Hsiou-Yu; Lin, Shiuan-Pey; Huang, Guan-Jhong; Lin, Wen-Chuan; Juang, Shin-Hun

    2016-01-01

    The whole plant of Anisomeles ovata has been widely used in Taiwan for treating inflammation-related skin and liver diseases, however, the detailed pharmacology mechanisms have yet to be elucidated. In the present study, one of the major components, 5,6,4'-trihydroxy-7,3'-dimethoxyflavone (5-TDMF), was purified from a methanol extract of Anisomeles ovata. A pharmacological study of this compound suggests that 5-TDMF possesses potent free radical scavenging activity both in vitro and ex vivo. Furthermore, 5-TDMF reduces nitric oxide and pro-inflammatory cytokine production in LPC-treated RAW 264.7 cells through the attenuation of nitric oxide synthase and cyclooxygenase-2. Additional experiments suggest that of 5-TDMF interferes with nuclear factor-κB translocation and mitogen-activated protein kinase pathways. These results identify 5-TDMF as an anti-oxidant and anti-inflammatory compound, explain the pharmacologic function of Anisomeles ovata and suggest its great potential as a new anti-inflammatory remedy. PMID:26805809

  11. Phonon spectra and heat capacity of Li2B4O7 and LiB3O5 crystals

    NASA Astrophysics Data System (ADS)

    Maslyuk, V. V.; Bredow, T.; Pfnür, H.

    2004-12-01

    The results of calculations of the phonon dispersion, the vibrational density of states and the heat capacity of lithium tetraborate and lithium triborate crystals are presented. They are obtained in the framework of a potential model that takes into account the non-equivalence of boron atoms in different structural positions (BO3 and BO4 units). A symmetry analysis of the phonon modes at Γ point was performed, and calculated frequencies are compared to experimental spectra. Analysis of Li contributions to the vibrational density of states reveals that the Li-O bonds in both crystals are relatively weak. This is in line with the experimentally observed high mobility of lithium ions at high temperatures. A good agreement between calculated and measured heat capacities from the literature was obtained.

  12. One-step synthesis, characterization and X-ray analysis of benzo[de]benzo[4,5]imidazo[2,1-a]isoquinolin-7-ones

    NASA Astrophysics Data System (ADS)

    Lu, Lihua; He, Liang

    2012-10-01

    Two isomeric compounds of methoxy substituted benzo[de]benzo[4,5]imidazo[2,1-a] -isoquinolin-7-one were obtained from the one-step ring closure reaction of 2-(2-acetamino-5-methoxyphenyl)benzo[de]isoquinoline-1,3-dione under acidic condition. Their structures were characterized by using HRMS, NMR spectra analyses together with single crystal X-ray diffraction. Compound 2 crystallizes in the monoclinic P2 (1)/n space group with the crystal cell parameters a = 8.3311(11) Å, b = 7.6646(10) Å, c = 21.201(2) Å, α = 90.00°, β = 95.7380(10)°, γ = 90.00°, V = 1347.0(3) Å3 and Z = 4. Compound 3 crystallizes in the monoclinic space group P2 (1)/c with the crystal cell parameters a = 3.8270(3) Å, b = 15.8361(14) Å, c = 28.559(3) Å, α = 90.00°, β = 94.1090(10)°, γ = 90.00°, V = 1726.4(3) Å3 and Z = 4. X-ray results indicated that it exist non-classically intermolecular interactions in the molecules of the two compounds. The substitution position of methoxy group has no obvious influence on the whole molecular planarity, but results in great different formation modes of their intermolecular interactions and structural stabilization.

  13. (+)-2-(1,2,3,4,4a,5,6,7-Octahydro-4a,8-dimethyl-7-oxo-2-naphthyl)propionic acid: catemeric hydrogen bonding in a bicyclic sesquiterpenoid keto acid.

    PubMed

    Brunskill, Andrew P J; Thompson, Hugh W; Lalancette, Roger A

    2002-05-01

    The title compound, C(15)H(22)O(3), derived from a naturally occurring sesquiterpenoid, has two molecules in the asymmetric unit, differing principally in the rotational conformation of the carboxyl group. Each species aggregates separately as a carboxyl-to-ketone hydrogen-bonding catemer [O.O = 2.752 (4) and 2.682 (4) A, and O-H.O = 161 (4) and 168 (4) degrees ], producing two crystallographically independent single-strand hydrogen-bonding helices, with opposite end-to-end orientations, passing through the cell in the b direction. Three intermolecular C-H.O=C close contacts exist for the ketone. PMID:11983981

  14. PIP2-dependent coupling is prominent in Kv7.1 due to weakened interactions between S4-S5 and S6

    NASA Astrophysics Data System (ADS)

    Kasimova, Marina A.; Zaydman, Mark A.; Cui, Jianmin; Tarek, Mounir

    2015-01-01

    Among critical aspects of voltage-gated potassium (Kv) channels' functioning is the effective communication between their two composing domains, the voltage sensor (VSD) and the pore. This communication, called coupling, might be transmitted directly through interactions between these domains and, as recently proposed, indirectly through interactions with phosphatidylinositol-4,5-bisphosphate (PIP2), a minor lipid of the inner plasma membrane leaflet. Here, we show how the two components of coupling, mediated by protein-protein or protein-lipid interactions, both contribute in the Kv7.1 functioning. On the one hand, using molecular dynamics simulations, we identified a Kv7.1 PIP2 binding site that involves residues playing a key role in PIP2-dependent coupling. On the other hand, combined theoretical and experimental approaches have shown that the direct interaction between the segments of the VSD (S4-S5) and the pore (S6) is weakened by electrostatic repulsion. Finally, we conclude that due to weakened protein-protein interactions, the PIP2-dependent coupling is especially prominent in Kv7.1.

  15. Interaction of helix D of elongation factor Tu with helices 4 and 5 of protein L7/12 on the ribosome.

    PubMed

    Kothe, Ute; Wieden, Hans-Joachim; Mohr, Dagmar; Rodnina, Marina V

    2004-03-01

    Elongation factor Tu (EF-Tu) promotes binding of aminoacyl-tRNA to the A site of the ribosome. Here, we report the effects of mutations in helix D of EF-Tu and in the C-terminal domain of L7/12 on the kinetics of A-site binding. Reaction rates were measured by stopped-flow and quench-flow techniques. The rates of A-site binding were decreased by mutations at positions 144, 145, 148, and 152 in helix D of EF-Tu as well as at positions 65, 66, 69, 70, 73, and 84 in helices 4 and 5 of L7/12. The effect was due primarily to the lower association rate constant of ternary complex binding to the ribosome. These results suggest that helix D of EF-Tu is involved in an initial transient contact with helices 4 and 5 of L7/12 that promotes ternary complex binding to the ribosome. By analogy to the interaction of helix D of EF-Tu with the N-terminal domain of EF-Ts, the contact area is likely to consist of a hydrophobic patch flanked by two salt-bridges. PMID:15037065

  16. PIP₂-dependent coupling is prominent in Kv7.1 due to weakened interactions between S4-S5 and S6.

    PubMed

    Kasimova, Marina A; Zaydman, Mark A; Cui, Jianmin; Tarek, Mounir

    2015-01-01

    Among critical aspects of voltage-gated potassium (Kv) channels' functioning is the effective communication between their two composing domains, the voltage sensor (VSD) and the pore. This communication, called coupling, might be transmitted directly through interactions between these domains and, as recently proposed, indirectly through interactions with phosphatidylinositol-4,5-bisphosphate (PIP₂), a minor lipid of the inner plasma membrane leaflet. Here, we show how the two components of coupling, mediated by protein-protein or protein-lipid interactions, both contribute in the Kv7.1 functioning. On the one hand, using molecular dynamics simulations, we identified a Kv7.1 PIP₂ binding site that involves residues playing a key role in PIP₂-dependent coupling. On the other hand, combined theoretical and experimental approaches have shown that the direct interaction between the segments of the VSD (S4-S5) and the pore (S6) is weakened by electrostatic repulsion. Finally, we conclude that due to weakened protein-protein interactions, the PIP2-dependent coupling is especially prominent in Kv7.1. PMID:25559286

  17. PIP2-dependent coupling is prominent in Kv7.1 due to weakened interactions between S4-S5 and S6

    PubMed Central

    Kasimova, Marina A.; Zaydman, Mark A.; Cui, Jianmin; Tarek, Mounir

    2015-01-01

    Among critical aspects of voltage-gated potassium (Kv) channels' functioning is the effective communication between their two composing domains, the voltage sensor (VSD) and the pore. This communication, called coupling, might be transmitted directly through interactions between these domains and, as recently proposed, indirectly through interactions with phosphatidylinositol-4,5-bisphosphate (PIP2), a minor lipid of the inner plasma membrane leaflet. Here, we show how the two components of coupling, mediated by protein-protein or protein-lipid interactions, both contribute in the Kv7.1 functioning. On the one hand, using molecular dynamics simulations, we identified a Kv7.1 PIP2 binding site that involves residues playing a key role in PIP2-dependent coupling. On the other hand, combined theoretical and experimental approaches have shown that the direct interaction between the segments of the VSD (S4–S5) and the pore (S6) is weakened by electrostatic repulsion. Finally, we conclude that due to weakened protein-protein interactions, the PIP2-dependent coupling is especially prominent in Kv7.1. PMID:25559286

  18. 3.6 and 4.5 μm Spitzer Phase Curves of the Highly Irradiated Hot Jupiters WASP-19b and HAT-P-7b

    NASA Astrophysics Data System (ADS)

    Wong, Ian; Knutson, Heather A.; Kataria, Tiffany; Lewis, Nikole K.; Burrows, Adam; Fortney, Jonathan J.; Schwartz, Joel; Shporer, Avi; Agol, Eric; Cowan, Nicolas B.; Deming, Drake; Désert, Jean-Michel; Fulton, Benjamin J.; Howard, Andrew W.; Langton, Jonathan; Laughlin, Gregory; Showman, Adam P.; Todorov, Kamen

    2016-06-01

    We analyze full-orbit phase curve observations of the transiting hot Jupiters WASP-19b and HAT-P-7b at 3.6 and 4.5 μm, obtained using the Spitzer Space Telescope. For WASP-19b, we measure secondary eclipse depths of 0.485%+/- 0.024% and 0.584%+/- 0.029% at 3.6 and 4.5 μm, which are consistent with a single blackbody with effective temperature 2372 ± 60 K. The measured 3.6 and 4.5 μm secondary eclipse depths for HAT-P-7b are 0.156%+/- 0.009% and 0.190%+/- 0.006%, which are well described by a single blackbody with effective temperature 2667 ± 57 K. Comparing the phase curves to the predictions of one-dimensional and three-dimensional atmospheric models, we find that WASP-19b’s dayside emission is consistent with a model atmosphere with no dayside thermal inversion and moderately efficient day–night circulation. We also detect an eastward-shifted hotspot, which suggests the presence of a superrotating equatorial jet. In contrast, HAT-P-7b’s dayside emission suggests a dayside thermal inversion and relatively inefficient day–night circulation; no hotspot shift is detected. For both planets, these same models do not agree with the measured nightside emission. The discrepancies in the model-data comparisons for WASP-19b might be explained by high-altitude silicate clouds on the nightside and/or high atmospheric metallicity, while the very low 3.6 μm nightside planetary brightness for HAT-P-7b may be indicative of an enhanced global C/O ratio. We compute Bond albedos of 0.38 ± 0.06 and 0 (\\lt 0.08 at 1σ ) for WASP-19b and HAT-P-7b, respectively. In the context of other planets with thermal phase curve measurements, we show that WASP-19b and HAT-P-7b fit the general trend of decreasing day–night heat recirculation with increasing irradiation.

  19. 7 CFR 276.5 - Injunctive relief.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 4 2011-01-01 2011-01-01 false Injunctive relief. 276.5 Section 276.5 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM STATE AGENCY LIABILITIES AND FEDERAL SANCTIONS §...

  20. 7 CFR 276.5 - Injunctive relief.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 4 2014-01-01 2014-01-01 false Injunctive relief. 276.5 Section 276.5 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM STATE AGENCY LIABILITIES AND FEDERAL SANCTIONS §...

  1. 7 CFR 276.5 - Injunctive relief.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 4 2013-01-01 2013-01-01 false Injunctive relief. 276.5 Section 276.5 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM STATE AGENCY LIABILITIES AND FEDERAL SANCTIONS §...

  2. 7 CFR 276.5 - Injunctive relief.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 4 2012-01-01 2012-01-01 false Injunctive relief. 276.5 Section 276.5 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM STATE AGENCY LIABILITIES AND FEDERAL SANCTIONS §...

  3. 7 CFR 276.5 - Injunctive relief.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 4 2010-01-01 2010-01-01 false Injunctive relief. 276.5 Section 276.5 Agriculture Regulations of the Department of Agriculture (Continued) FOOD AND NUTRITION SERVICE, DEPARTMENT OF AGRICULTURE FOOD STAMP AND FOOD DISTRIBUTION PROGRAM STATE AGENCY LIABILITIES AND FEDERAL SANCTIONS §...

  4. Non-additive hepatic gene expression elicited by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153) co-treatment in C57BL/6 mice

    SciTech Connect

    Kopec, Anna K.; D'Souza, Michelle L.; Mets, Bryan D.; Burgoon, Lyle D.; Reese, Sarah E.; Archer, Kellie J.; Potter, Dave; Tashiro, Colleen; Sharratt, Bonnie; Harkema, Jack R.; Zacharewski, Timothy R.

    2011-10-15

    Interactions between environmental contaminants can lead to non-additive effects that may affect the toxicity and risk assessment of a mixture. Comprehensive time course and dose-response studies with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), non-dioxin-like 2,2',4,4',5,5'-hexachlorobiphenyl (PCB153) and their mixture were performed in immature, ovariectomized C57BL/6 mice. Mice were gavaged once with 30 {mu}g/kg TCDD, 300 mg/kg PCB153, a mixture of 30 {mu}g/kg TCDD with 300 mg/kg PCB153 (MIX) or sesame oil vehicle for 4,12, 24,72 or 168 h. In the 24 h dose-response study, animals were gavaged with TCDD (0.3,1, 3, 6, 10, 15, 30, 45 {mu}g/kg), PCB153 (3,10, 30, 60, 100, 150, 300, 450 mg/kg), MIX (0.3 + 3, 1 + 10, 3 + 30, 6 + 60, 10 + 100, 15 + 150, 30 + 300, 45 {mu}g/kg TCDD + 450 mg/kg PCB153, respectively) or vehicle. All three treatments significantly increased relative liver weights (RLW), with MIX eliciting significantly greater increases compared to TCDD and PCB153 alone. Histologically, MIX induced hepatocellular hypertrophy, vacuolization, inflammation, hyperplasia and necrosis, a combination of TCDD and PCB153 responses. Complementary lipid analyses identified significant increases in hepatic triglycerides in MIX and TCDD samples, while PCB153 had no effect on lipids. Hepatic PCB153 levels were also significantly increased with TCDD co-treatment. Microarray analysis identified 167 TCDD, 185 PCB153 and 388 MIX unique differentially expressed genes. Statistical modeling of quantitative real-time PCR analysis of Pla2g12a, Serpinb6a, Nqo1, Srxn1, and Dysf verified non-additive expression following MIX treatment compared to TCDD and PCB153 alone. In summary, TCDD and PCB153 co-treatment elicited specific non-additive gene expression effects that are consistent with RLW increases, histopathology, and hepatic lipid accumulation. - Graphical abstract: Display Omitted Highlights: > MIX (TCDD:PCB153 at 1:10,000 ratio) exposure leads to non-additive gene expression

  5. Structure and dielectric properties of solid solutions Bi7Ti4 + x W x Nb1 - 2 x O21 ( x = 0-0.5)

    NASA Astrophysics Data System (ADS)

    Vlasenko, V. G.; Zubkov, S. V.; Shuvaeva, V. A.

    2015-05-01

    The structural and electrophysical characteristics of a series of solid solutions of layered perovskite-type oxides Bi7Ti4 + x W x Nb1 - 2 x O21 ( x = 0-0.5) have been investigated. According to X-ray powder dif- fraction data, all the studied compounds are single-phase and have the structure of Aurivillius phases ( m = 2.5) with an orthorhombic crystal lattice (space group I2 cm, Z = 2). The changes in the tetragonal and orthorhombic distortions of perovskite-like layers in the compounds have been considered depending on their chemical composition. The temperature dependences of the relative permittivity ɛ( T) have been measured. It has been shown that the Curie temperature T C of the perovskite-type oxides Bi7Ti4 + x W x Nb1 - 2 x O21 ( x = 0-0.5) decreases linearly with an increase in the parameter x. The activation energies of charge carriers have been obtained in different temperature ranges. It has been found that there are three temperature regions with very different activation energies due to different natures of charge carriers in the studied compounds.

  6. Novel 3-Amino-6-chloro-7-(azol-2 or 5-yl)-1,1-dioxo-1,4,2-benzodithiazine Derivatives with Anticancer Activity: Synthesis and QSAR Study.

    PubMed

    Pogorzelska, Aneta; Sławiński, Jarosław; Brożewicz, Kamil; Ulenberg, Szymon; Bączek, Tomasz

    2015-01-01

    A series of new 3-amino-6-chloro-7-(azol-2 or 5-yl)-1,1-dioxo-1,4,2-benzodithiazine derivatives 5a-j have been synthesized and evaluated in vitro for their antiproliferative activity at the U.S. National Cancer Institute. The most active compound 5h showed significant cytotoxic effects against ovarian (OVCAR-3) and breast (MDA-MB-468) cancer (10% and 47% cancer cell death, respectively) as well as a good selectivity toward prostate (DU-145), colon (SW-620) and renal (TK-10) cancer cell lines. To obtain a deeper insight into the structure-activity relationships of the new compounds 5a-j QSAR studies have been applied. Theoretical calculations allowed the identification of molecular descriptors belonging to the RDF (RDF055p and RDF145m in the MOLT-4 and UO-31 QSAR models, respectively) and 3D-MorSE (Mor32m and Mor16e for MOLT-4 and UO-31 QSAR models) descriptor classes. Based on these data, QSAR models with good robustness and predictive ability have been obtained. PMID:26690109

  7. 5,6,7,8-Tetrahydropyrido[4,3-d]pyrimidines as novel class of potent and highly selective CaMKII inhibitors.

    PubMed

    Asano, Shigehiro; Komiya, Masafumi; Koike, Nobuyuki; Koga, Erina; Nakatani, Shogo; Isobe, Yoshiaki

    2010-11-15

    A novel series of 5,6,7,8-tetrahydropyrido[4,3-d]pyrimidines containing substituted phenyl sulfonamide are synthesized and evaluated for their inhibitory activity against CaMKII. Substituents on the phenyl group had significant impact on CaMKII inhibition, in particular, the inhibitory activity of 8p was 25-fold higher than that of KN-93, a known CaMKII inhibitor. Michaelis-Menten analysis of a representative compound suggested that the synthesized pyrimidines are calmodulin non-competitive inhibitors. Finally, 8p exhibited more than 100-fold higher selectivity for CaMKII over five types of off-target kinases. PMID:20875738

  8. 3,5,6,7,8,3',4'-Heptamethoxyflavone, a citrus flavonoid, on protection against memory impairment and neuronal cell death in a global cerebral ischemia mouse model.

    PubMed

    Okuyama, Satoshi; Morita, Mayu; Miyoshi, Kazuhiro; Nishigawa, Yuki; Kaji, Miki; Sawamoto, Atsushi; Terugo, Tsukasa; Toyoda, Nobuki; Makihata, Nahomi; Amakura, Yoshiaki; Yoshimura, Morio; Nakajima, Mitsunari; Furukawa, Yoshiko

    2014-05-01

    The present study evaluated the effects of treatment with the citrus flavonoid, 3,5,6,7,8,3',4'-heptamethoxyflavone (HMF) on protection against memory impairment and neuronal death in a global cerebral ischemia mouse model. The results showed that HMF, administrated for three days immediately after ischemic surgery, protected against ischemia-induced memory dysfunction, rescued neuronal cell death in the CA1 cell layer, increased the production of BDNF, stimulated the autophosphorylation of CaMK II and suppressed microglial activation in the hippocampus. These results suggest that HMF has a neuroprotective effect after brain ischemia by inducing BDNF production and anti-inflammatory effects. PMID:24657445

  9. Facile synthesis of 4,5,6a,7-tetrahydrodibenzo[de,g]chromene heterocycles and their transformation to phenanthrene alkaloids

    PubMed Central

    Kapadia, Nirav; Harding, Wayne

    2013-01-01

    Oxa-Pictet-Spengler cyclization and microwave-assisted C-H arylation have been implemented as key steps in the synthesis of new isochroman heterocycles containing a 4,5,6a,7-tetrahydrodibenzo[de,g]chromene motif. These isochromans may be easily transformed to phenanthrene alkaloids via acidic cleavage of the isochroman ring and standard synthetic manipulations thereafter. The route described is attractive in that it provides access to two biologically interesting scaffolds in simple and high yielding synthetic steps. PMID:24187388

  10. Metal Complexes of Dithiolate Ligands: 5,6-Dihydro-1,4-dithiin-2,3-dithiolato (dddt(2-)), 5,7-Dihydro-1,4,6-trithiin-2,3-dithiolato (dtdt(2-)), and 2-Thioxo-1,3-dithiole-4,5-dithiolato (dmit(2-)). Synthesis, Electrochemical Studies, Crystal and Electronic Structures, and Conducting Properties.

    PubMed

    Faulmann, Christophe; Errami, Ahmed; Donnadieu, Bruno; Malfant, Isabelle; Legros, Jean-Pierre; Cassoux, Patrick; Rovira, Carme; Canadell, Enric

    1996-06-19

    New precursors to potentially conductive noninteger oxidation state (NIOS) compounds based on metal complexes [ML(2)](n)()(-) [M = Ni, Pd, Pt; L = 5,6-dihydro-1,4-dithiin-2,3-dithiolato (dddt(2)(-)), 5,7-dihydro-1,4,6-trithiin-2,3-dithiolato (dtdt(2)(-)), and 2-thioxo-1,3-dithiole-4,5-dithiolato (dmit(2)(-)); n = 2, 1, 0] have been investigated. Complexes of the series (NR(4))[ML(2)] (R = Me, Et, Bu; L = dddt(2)(-), dtdt(2)(-)) have been isolated and characterized, and the crystal structure of (NBu(4))[Pt(dtdt)(2)] (1) has been determined {1 = C(24)H(44)NPtS(10), a = 12.064(2) Å, b = 17.201(3) Å, c = 16.878(2) Å, beta = 102.22(2) degrees, V = 3423(1) Å(3), monoclinic, P2(1)/n, Z = 4}. Oxidation of these complexes affords the corresponding neutral species [ML(2)](0). Another series of general formula (cation)(n)()[M(dmit)(2)] [cation = PPN(+), BTP(+), and (SMe(y)()Et(3)(-)(y)())(+) with y = 0, 1, 2, and 3, n = 2, 1, M = Ni, Pd] has also been studied. All of these (cation)(n)()[M(dmit)(2)] complexes have been isolated and characterized [with the exception of (cation)[Pd(dmit)(2)] for cation = (SMe(y)()Et(3)(-)(y)())(+)]. The crystal structures of (PPN)[Ni(dmit)(2)].(CH(3))(2)CO (2) and (SMeEt(2))[Ni(dmit)(2)] (3) have been determined {2 = C(45)H(36)NNiS(10)P(2)O, a = 12.310(2) Å, b = 13.328(3) Å, c = 15.850(3) Å, alpha = 108.19(3) degrees, beta = 96.64(2) degrees, gamma = 99.67(2) degrees, V = 2373(1) Å(3), triclinic, P&onemacr;, Z = 2; 3 = C(11)H(13)NiS(11), a = 7.171(9) Å, b = 17.802(3) Å, c = 16.251(3) Å, beta = 94.39(4) degrees, V = 2068(2) Å(3), monoclinic, P2(1)/n, Z = 4} NIOS salts derived from the preceding precursors were obtained by electrochemical oxidation. Electrochemical studies of the [M(dddt)(2)] complexes show that they may be used for the preparation of NIOS radical cation salts and [M(dddt)(2)][M'(dmit)(2)](x)() compounds, but not for the preparation of (cation)[M(dddt)(2)](z)() NIOS radical anion salts. The electrochemical oxidation of

  11. Solid-phase synthesis of 2-substituted 4-amino-7-oxo-5,6,7,8-tetrahydropyrido[2,3-d]pyrimidines.

    PubMed

    Falcó, José L; Borrell, José I; Teixidó, Jordi

    2003-01-01

    An efficient solid-phase synthesis of 2-substituted 4-aminopyrido[2,3-d]pyrimidines 15 is reported. The procedure started by solid supporting a p-hydroxybenzaldehyde 8 to the Wang resin by using the Mitsunobu protocol. The resulting aldehyde 17 was treated with a substituted acid methyl malonate 10 to afford the corresponding alpha, beta-unsaturated ester 18, which was converted to the Michael adduct 21 by reaction with malononitrile. Cyclization of 21 with an amidine system 13 yielded the solid supported pyridopyrimidine 22, which afforded the corresponding 2-substituted 4-aminopyrido[2,3-d]pyrimidine 15 upon treatment with TFA:DCM. Compounds 15 present three diversity centers R1, R2 and R3. Having validated the chemistry on solid support, a 32-membered combinatorial library was obtained using this protocol. PMID:14761159

  12. Crystal structures of three anhydrous salts of the Lewis base 1,8-di-aza-bicyclo-[5.4.0]undec-7-ene (DBU) with the ring-substituted benzoic acid analogues 4-amino-benzoic acid, 3,5-di-nitro-benzoic acid and 3,5-di-nitro-salicylic acid.

    PubMed

    Smith, Graham; Lynch, Daniel E

    2016-03-01

    The anhydrous salts of the Lewis base 1,8-di-aza-bicyclo-[5.4.0]undec-7-ene (DBU) with 4-amino-benzoic acid [1-aza-8-azoniabi-cyclo-[5.4.0]undec-7-ene 4-amino-benzoate, C9H17N2 (+)·C7H6NO2 (-) (I)], 3,5-di-nitro-benzoic acid [1-aza-8-azoniabi-cyclo-[5.4.0]undec-7-ene 3,5-di-nitro-benzoate, C9H17N2 (+)·C7H3N2O6 (-), (II)] and 3,5-di-nitro-salicylic acid (DNSA) [1-aza-8-azoniabi-cyclo-[5.4.0]undec-7-ene 2-hy-droxy-3,5-di-nitro-benzoate, C9H17N2 (+)·C7H3N2O7 (-), (III)] have been determined and their hydrogen-bonded structures are described. In both (II) and (III), the DBU cations have a common disorder in three of the C atoms of the six-membered ring moieties [site-occupancy factors (SOF) = 0.735 (3)/0.265 (3) and 0.686 (4)/0.314 (4), respectively], while in (III), there is additional rotational disorder in the DNSA anion, giving two sites (SOF = 0.72/0.28, values fixed) for the phenol group. In the crystals of (I) and (III), the cation-anion pairs are linked through a primary N-H⋯Ocarbox-yl hydrogen bond [2.665 (2) and 2.869 (3) Å, respectively]. In (II), the ion pairs are linked through an asymmetric three-centre R 1 (2)(4), N-H⋯O,O' chelate association. In (I), structure extension is through amine N-H⋯Ocarbox-yl hydrogen bonds between the PABA anions, giving a three-dimensional structure. The crystal structures of (II) and (III) are very similar, the cation-anion pairs being associated only through weak C-H⋯O hydrogen bonds, giving in both overall two-dimensional layered structures lying parallel to (001). No π-π ring associations are present in any of the structures. PMID:27006813

  13. Crystal structures of three anhydrous salts of the Lewis base 1,8-di­aza­bicyclo­[5.4.0]undec-7-ene (DBU) with the ring-substituted benzoic acid analogues 4-amino­benzoic acid, 3,5-di­nitro­benzoic acid and 3,5-di­nitro­salicylic acid

    PubMed Central

    Smith, Graham; Lynch, Daniel E.

    2016-01-01

    The anhydrous salts of the Lewis base 1,8-di­aza­bicyclo­[5.4.0]undec-7-ene (DBU) with 4-amino­benzoic acid [1-aza-8-azoniabi­cyclo­[5.4.0]undec-7-ene 4-amino­benzoate, C9H17N2 +·C7H6NO2 − (I)], 3,5-di­nitro­benzoic acid [1-aza-8-azoniabi­cyclo­[5.4.0]undec-7-ene 3,5-di­nitro­benzoate, C9H17N2 +·C7H3N2O6 −, (II)] and 3,5-di­nitro­salicylic acid (DNSA) [1-aza-8-azoniabi­cyclo­[5.4.0]undec-7-ene 2-hy­droxy-3,5-di­nitro­benzoate, C9H17N2 +·C7H3N2O7 −, (III)] have been determined and their hydrogen-bonded structures are described. In both (II) and (III), the DBU cations have a common disorder in three of the C atoms of the six-membered ring moieties [site-occupancy factors (SOF) = 0.735 (3)/0.265 (3) and 0.686 (4)/0.314 (4), respectively], while in (III), there is additional rotational disorder in the DNSA anion, giving two sites (SOF = 0.72/0.28, values fixed) for the phenol group. In the crystals of (I) and (III), the cation–anion pairs are linked through a primary N—H⋯Ocarbox­yl hydrogen bond [2.665 (2) and 2.869 (3) Å, respectively]. In (II), the ion pairs are linked through an asymmetric three-centre R 1 2(4), N—H⋯O,O′ chelate association. In (I), structure extension is through amine N—H⋯Ocarbox­yl hydrogen bonds between the PABA anions, giving a three-dimensional structure. The crystal structures of (II) and (III) are very similar, the cation–anion pairs being associated only through weak C—H⋯O hydrogen bonds, giving in both overall two-dimensional layered structures lying parallel to (001). No π–π ring associations are present in any of the structures. PMID:27006813

  14. Synthesis and identification of a new class of (S)-2,6-diamino-4,5,6,7-tetrahydrobenzo[d]thiazole derivatives as potent antileukemic agents.

    PubMed

    Prasanna, D S; Kavitha, C V; Raghava, B; Vinaya, K; Ranganatha, S R; Raghavan, Sathees C; Rangappa, K S

    2010-08-01

    Benzothiazoles are multitarget agents with broad spectrum of biological activity. Among the antitumor agents discovered in recent years, the identification of various 2-(4-aminophenyl) benzothiazoles as potent and selective antitumor drugs against different cancer cell lines has stimulated remarkable interest. Some of the benzothiazoles are known to induce cell cycle arrest, activation of caspases and interaction with DNA molecule. Based on these interesting properties of benzothiazoles and to obtain new biologically active agents, a series of novel 4,5,6,7-tetrahydrobenzo[d]thiazole derivatives 5(a-i) were synthesized and evaluated for their efficacy as antileukemic agents in human leukemia cells (K562 and Reh). The chemical structures of the synthesized compounds were confirmed by (1)H NMR, LCMS and IR analysis. The cytotoxicity of these compounds were determined using trypan blue exclusion, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. Results showed that, these compounds mediate a significant cytotoxic response to cancer cell lines tested. We found that the compounds having electron withdrawing groups at different positions of the phenyl ring of the thiourea moiety displayed significant cytotoxic effect with IC(50) value less than 60 microM. To rationalize the role of electron withdrawing group in the induction of cytotoxicity, we have chosen molecule 5g (IC(50) approximately 15 microM) which is having chloro substitution at ortho and para positions. Flow cytometric analysis of annexin V-FITC/ propidium iodide (PI) double staining and DNA fragmentation suggest that 5g can induce apoptosis. PMID:19506804

  15. Formation and characterization of microstructure of as-cast Mg–6Gd–4Y–xZn–0.5Zr (x = 0.3, 0.5 and 0.7 wt.%) alloys

    SciTech Connect

    Wu, Y.J.; Xu, C.; Zheng, F.Y.; Peng, L.M.; Zhang, Y.; Ding, W.J.

    2013-05-15

    Mg–6Gd–4Y–xZn–0.5Zr (x = 0.3, 0.5 and 0.7 wt.%) alloys were prepared via conventional ingot metallurgy (I/M) in this study. The as-cast microstructures of these alloys were established by X-ray diffraction (XRD) analyses, optical microscope (OM), scanning electron microscope (SEM) and transmission electron microscope (TEM) observations. Lamellar stacking order (SF) and 14H-type long period stacking order (LPSO) structure within α-Mg matrix are formed in the three as-cast alloys. The eutectic secondary phase is (Mg,Zn){sub 24}(Gd,Y){sub 5} for the alloy containing 0.3 wt.% Zn, while, it is (Mg,Zn){sub 3}(Gd,Y) for the alloys containing 0.5 wt.% Zn and 0.7 wt.% Zn. Moreover, X phase-(Mg,Zn){sub 12}(Gd,Y) is formed in the latter two as-cast alloys. - Highlights: • LPSO structure has first been found in as-cast Mg–6Gd–4Y–xZn–0.5Zr. • X-phase exists in as-cast Mg–6Gd–4Y–0.3(0.5)Zn–0.5Zr. • Zn content results in different β-phase in the studied alloys.

  16. Synthesis of 4-((1E, 6E)-7-(4-hydroxy-3-methoxyphenyl)-3, 5-dioxohepta-1, 6-dienyl)-2-methoxyphenyl 4-fluorobenzoate, a novel monoester derivative of curcumin, its experimental and theoretical (DFT) studies

    NASA Astrophysics Data System (ADS)

    Srivastava, Sangeeta; Gupta, Preeti; Amandeep; Singh, Ranvijay Pratap

    2016-04-01

    Curcumin (1), isolated as a major component from the chloroform extract of Curcuma longa was converted to its ester derivative 4-((1E, 6E)-7-(4-hydroxy-3-methoxyphenyl)-3,5-dioxohepta-1,6-dienyl)-2-methoxyphenyl 4-fluorobenzoate (2). The compound has been characterized with the help of 1H, 13C NMR, UV, IR and mass spectrometry. The molecular geometry of synthesized compound was calculated in ground state by Density functional theory (DFT/B3LYP) using 6-31G (d,p) basis set. 1H and 13C NMR chemical shifts were calculated in ground state by using Gauge-Including Atomic Orbital (GIAO) approach and these values were correlated with experimental observations. The electronic properties such as HOMO and LUMO energies were calculated using time dependent Density Functional Theory (TD-DFT). Stability of the molecule as a result of hyper conjugative interactions and electron delocalization were analysed using Natural bond orbital (NBO) analysis. Intramolecular interactions were analysed by AIM (Atom in molecule) approach. Global reactivity descriptors were calculated to study the reactive site within molecule. The vibrational wavenumbers were calculated using DFT method and assigned with the help of potential energy distribution (PED). First hyperpolarizability values have been calculated to describe the nonlinear optical (NLO) property of the synthesized compounds. Molecular electrostatic potential (MEP) analysis has also been carried out.

  17. Full protection against African horsesickness (AHS) in horses induced by baculovirus-derived AHS virus serotype 4 VP2, VP5 and VP7.

    PubMed

    Martínez-Torrecuadrada, J L; Díaz-Laviada, M; Roy, P; Sánchez, C; Vela, C; Sánchez-Vizcaíno, J M; Casal, J I

    1996-06-01

    African horsesickness virus serotype 4 (AHSV-4) outer capsid protein VP2, or VP2 and VP5 plus inner capsid protein VP7, derived from single or dual recombinant baculovirus expression vectors were used in different combinations to immunize horses. When the proteins were purified by affinity chromatography, the combination of all three proteins induced low levels of neutralizing antibodies and conferred protection against virulent virus challenge. However, purified VP2 or VP2 and VP5 in the absence of VP7 failed to induce neutralizing antibodies and protection. Immunization with non-purified proteins enhanced the titres of neutralizing antibodies. Again, the combination of the three proteins was able to confer total protection to immunized horses, which showed absence of viraemia. The antigenicity of recombinant VP2 was analysed with a collection of 30 MAbs. Both purified and unpurified recombinant VP2 proteins showed different antigenic patterns in comparison to that of VP2 on virions. An immunization experiment with four more horses confirmed these results. The vaccine described here would not only prevent the disease, but would drastically reduce the propagation of the virus by vectors. PMID:8683209

  18. Solid state structure by X-ray and 13C CP/MAS NMR of new 5-[2-(N,N-dimethylamino)ethoxy]-4,7-dimethylcoumarins

    NASA Astrophysics Data System (ADS)

    Ostrowska, Kinga; Maciejewska, Dorota; Dobrzycki, Łukasz; Socha, Pawel

    2016-05-01

    5-[2-(N,N-dimethylamino)ethoxy]-4,7-dimethylcoumarin (1) and 6-acetyl-5-[2-(N,N-dimethylamino)ethoxy]-4,7-dimethylcoumarin (2), structurally related, were synthesized using both conventional and microwave-assisted approach. An impact of acetyl groups on the molecular structure of coumarin derivatives has been examined. Crystals of 2 were investigated using single crystal and powder X-ray diffraction techniques. Compound 2 crystallizes forming two polymorphs (denoted as 2_1 and 2_2), both belonging to P21/c space group. Both polymorphs are comparably stable and can be formed simultaneously during crystallization process. The solid state structure was also analysed using the fully resolved 13C CP/MAS NMR. The double signals with the intensity ratio of about 1:1 which were observed in the 13C CP/MAS NMR spectrum of compound 1 must arise due to the presence of two conformers of 1. In contrast, NMR spectrum recorded for powder mixture of two polymorphs of compound 2 displays no signal splitting. This is related to structural similarities of molecules in both polymorphs.

  19. 7-(Pyrazol-4-yl)-3H-imidazo[4,5-b]pyridine-based derivatives for kinase inhibition: Co-crystallisation studies with Aurora-A reveal distinct differences in the orientation of the pyrazole N1-substituent.

    PubMed

    Bavetsias, Vassilios; Pérez-Fuertes, Yolanda; McIntyre, Patrick J; Atrash, Butrus; Kosmopoulou, Magda; O'Fee, Lisa; Burke, Rosemary; Sun, Chongbo; Faisal, Amir; Bush, Katherine; Avery, Sian; Henley, Alan; Raynaud, Florence I; Linardopoulos, Spiros; Bayliss, Richard; Blagg, Julian

    2015-10-01

    Introduction of a 1-benzyl-1H-pyrazol-4-yl moiety at C7 of the imidazo[4,5-b]pyridine scaffold provided 7a which inhibited a range of kinases including Aurora-A. Modification of the benzyl group in 7a, and subsequent co-crystallisation of the resulting analogues with Aurora-A indicated distinct differences in binding mode dependent upon the pyrazole N-substituent. Compounds 7a and 14d interact with the P-loop whereas 14a and 14b engage with Thr217 in the post-hinge region. These crystallographic insights provide options for the design of compounds interacting with the DFG motif or with Thr217. PMID:26296477

  20. Novel Phenyl-Substituted 5,6-Dihydro-[1,2,4]triazolo[4,3-a]pyrazine P2X7 Antagonists with Robust Target Engagement in Rat Brain.

    PubMed

    Chrovian, Christa C; Soyode-Johnson, Akinola; Ao, Hong; Bacani, Genesis M; Carruthers, Nicholas I; Lord, Brian; Nguyen, Leslie; Rech, Jason C; Wang, Qi; Bhattacharya, Anindya; Letavic, Michael A

    2016-04-20

    Novel 5,6-dihydro-[1,2,4]triazolo[4,3-a]pyrazine P2X7 antagonists were optimized to allow for good blood-brain barrier permeability and high P2X7 target engagement in the brain of rats. Compound 25 (huP2X7 IC50 = 9 nM; rat P2X7 IC50 = 42 nM) achieved 80% receptor occupancy for 6 h when dosed orally at 10 mg/kg in rats as measured by ex vivo radioligand binding autoradiography. Structure-activity relationships within this series are described, as well as in vitro ADME results. In vivo pharmacokinetic data for key compounds is also included. PMID:26752113

  1. Discovery of 1-(4-methoxyphenyl)-7-oxo-6-(4-(2-oxopiperidin-1-yl)phenyl)-4,5,6,7-tetrahydro-1H-pyrazolo[3,4-c]pyridine-3-carboxamide (apixaban, BMS-562247), a highly potent, selective, efficacious, and orally bioavailable inhibitor of blood coagulation factor Xa.

    PubMed

    Pinto, Donald J P; Orwat, Michael J; Koch, Stephanie; Rossi, Karen A; Alexander, Richard S; Smallwood, Angela; Wong, Pancras C; Rendina, Alan R; Luettgen, Joseph M; Knabb, Robert M; He, Kan; Xin, Baomin; Wexler, Ruth R; Lam, Patrick Y S

    2007-11-01

    Efforts to identify a suitable follow-on compound to razaxaban (compound 4) focused on modification of the carboxamido linker to eliminate potential in vivo hydrolysis to a primary aniline. Cyclization of the carboxamido linker to the novel bicyclic tetrahydropyrazolopyridinone scaffold retained the potent fXa binding activity. Exceptional potency of the series prompted an investigation of the neutral P1 moieties that resulted in the identification of the p-methoxyphenyl P1, which retained factor Xa binding affinity and good oral bioavailability. Further optimization of the C-3 pyrazole position and replacement of the terminal P4 ring with a neutral heterocycle culminated in the discovery of 1-(4-methoxyphenyl)-7-oxo-6-(4-(2-oxopiperidin-1-yl)phenyl)-4,5,6,7-tetrahydro-1H-pyrazolo[3,4-c]pyridine-3-carboxamide (apixaban, compound 40). Compound 40 exhibits a high degree of fXa potency, selectivity, and efficacy and has an improved pharmacokinetic profile relative to 4. PMID:17914785

  2. Discovery of 1-(4-Methoxyphenyl)-7-oxo-6-(4-(2-oxopiperidin-1-yl)phenyl)-4,5,6,7-tetrahydro- 1H-pyrazolo[3,4-c]pyridine-3-carboxamide (Apixaban, BMS-562247), a Highly Potent, Selective, Efficacious, and Orally Bioavailable Inhibitor of Blood Coagulation Factor Xa

    SciTech Connect

    Pinto, Donald J.P.; Orwat, Michael J.; Koch, Stephanie; Rossi, Karen A.; Alexander, Richard S.; Smallwood, Angela; Wong, Pancras C.; Rendina, Alan R.; Luettgen, Joseph M.; Knabb, Robert M.; He, Kan; Xin, Baomin; Wexler, Ruth R.; Lam, Patrick Y.S.

    2010-03-08

    Efforts to identify a suitable follow-on compound to razaxaban (compound 4) focused on modification of the carboxamido linker to eliminate potential in vivo hydrolysis to a primary aniline. Cyclization of the carboxamido linker to the novel bicyclic tetrahydropyrazolopyridinone scaffold retained the potent fXa binding activity. Exceptional potency of the series prompted an investigation of the neutral P{sub 1} moieties that resulted in the identification of the p-methoxyphenyl P{sub 1}, which retained factor Xa binding affinity and good oral bioavailability. Further optimization of the C-3 pyrazole position and replacement of the terminal P{sub 4} ring with a neutral heterocycle culminated in the discovery of 1-(4-methoxyphenyl)-7-oxo-6-(4-(2-oxopiperidin-1-yl)phenyl)-4,5,6,7-tetrahydro-1H-pyrazolo[3,4-c]pyridine-3-carboxamide (apixaban, compound 40). Compound 40 exhibits a high degree of fXa potency, selectivity, and efficacy and has an improved pharmacokinetic profile relative to 4.

  3. Photophysical properties of ESIPT inspired fluorescent 2-(2-hydroxyphenyl)-6-methylimidazo[4,5-f]isoindole-5,7(1H,6H)-dione and its derivative: experimental and DFT based approach.

    PubMed

    Deshmukh, Mininath S; Sekar, Nagaiyan

    2015-01-25

    The excited-state intramolecular proton transfer chromophores 2-(2-hydroxyphenyl)-6-methylimidazo[4,5-f]isoindole-5,7(1H,6H)-dione and 2-(4-(diethylamino)-2-hydroxyphenyl)-6-methylimidazo[4,5-f]isoindole-5,7(1H,6H)-dione are synthesized from 4,5-diamino-N-methylphthalimide. The photophysical behavior of the synthesized chromophores was studied using UV-visible and fluorescence spectroscopy in the polar and non-polar solvents. The synthesized o-hydroxyphenyl benzimidazole derivatives are fluorescent and very sensitive to the solvent polarity. These dyes are thermally stable up to 317 °C. Density Functional Theory computations have been used to understand the structural, molecular, electronic and photophysical properties of the chromophores. The experimental absorption and emission wavelengths are in good agreement with the computed vertical excitation and theoretical emission obtained by Density Functional Theory and Time Dependant Density Functional Theory. PMID:25108369

  4. 7 CFR 371.4 - Veterinary Services.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 5 2013-01-01 2013-01-01 false Veterinary Services. 371.4 Section 371.4 Agriculture..., DEPARTMENT OF AGRICULTURE ORGANIZATION, FUNCTIONS, AND DELEGATIONS OF AUTHORITY § 371.4 Veterinary Services. (a) General statement. Veterinary Services (VS) protects and safeguards the Nation's livestock...

  5. 7 CFR 353.4 - Products covered.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 5 2011-01-01 2011-01-01 false Products covered. 353.4 Section 353.4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORT CERTIFICATION § 353.4 Products covered. Plants and plant products...

  6. 7 CFR 319.73-4 - Costs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Costs. 319.73-4 Section 319.73-4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Coffee § 319.73-4 Costs. All costs of...

  7. 7 CFR 353.4 - Products covered.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 5 2012-01-01 2012-01-01 false Products covered. 353.4 Section 353.4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORT CERTIFICATION § 353.4 Products covered. Plants and plant products...

  8. 7 CFR 319.73-4 - Costs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 5 2013-01-01 2013-01-01 false Costs. 319.73-4 Section 319.73-4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Coffee § 319.73-4 Costs. All costs of...

  9. 7 CFR 319.73-4 - Costs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

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  10. 7 CFR 319.73-4 - Costs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 5 2014-01-01 2014-01-01 false Costs. 319.73-4 Section 319.73-4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Coffee § 319.73-4 Costs. All costs of...

  11. 7 CFR 319.73-4 - Costs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 5 2012-01-01 2012-01-01 false Costs. 319.73-4 Section 319.73-4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Coffee § 319.73-4 Costs. All costs of...

  12. 7 CFR 352.4 - Documentation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Documentation. 352.4 Section 352.4 Agriculture..., DEPARTMENT OF AGRICULTURE PLANT QUARANTINE SAFEGUARD REGULATIONS § 352.4 Documentation. (a) Manifest... for examination a complete manifest or other documentation from which the inspector may...

  13. 7 CFR 319.75-4 - Treatments.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 5 2012-01-01 2012-01-01 false Treatments. 319.75-4 Section 319.75-4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Khapra Beetle § 319.75-4 Treatments. A...

  14. 7 CFR 319.75-4 - Treatments.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Treatments. 319.75-4 Section 319.75-4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Khapra Beetle § 319.75-4 Treatments. A...

  15. 7 CFR 319.75-4 - Treatments.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 5 2014-01-01 2014-01-01 false Treatments. 319.75-4 Section 319.75-4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Khapra Beetle § 319.75-4 Treatments. A...

  16. 7 CFR 319.75-4 - Treatments.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 5 2011-01-01 2011-01-01 false Treatments. 319.75-4 Section 319.75-4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Khapra Beetle § 319.75-4 Treatments. A...

  17. 7 CFR 319.75-4 - Treatments.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 5 2013-01-01 2013-01-01 false Treatments. 319.75-4 Section 319.75-4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FOREIGN QUARANTINE NOTICES Khapra Beetle § 319.75-4 Treatments. A...

  18. 7 CFR 353.4 - Products covered.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 5 2010-01-01 2010-01-01 false Products covered. 353.4 Section 353.4 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORT CERTIFICATION § 353.4 Products covered. Plants and plant products...

  19. Structure-activity relationship study of selective excitatory amino acid transporter subtype 1 (EAAT1) inhibitor 2-amino-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-5,6,7,8-tetrahydro-4H-chromene-3-carbonitrile (UCPH-101) and absolute configurational assignment using infrared and vibrational circular dichroism spectroscopy in combination with ab initio Hartree-Fock calculations.

    PubMed

    Huynh, Tri H V; Shim, Irene; Bohr, Henrik; Abrahamsen, Bjarke; Nielsen, Birgitte; Jensen, Anders A; Bunch, Lennart

    2012-06-14

    The excitatory amino acid transporters (EAATs) play essential roles in regulating the synaptic concentration of the neurotransmitter glutamate in the mammalian central nervous system. To date, five subtypes have been identified, named EAAT1-5 in humans, and GLAST, GLT-1, EAAC1, EAAT4, and EAAT5 in rodents, respectively. In this paper, we present the design, synthesis, and pharmacological evaluation of seven 7-N-substituted analogues of UCPH-101/102. Analogue 9 inhibited EAAT1 in the micromolar range (IC(50) value 20 μM), whereas analogues 8 and 10 were inactive (IC(50) values >100 μM). The diastereomeric pairs 11a/11b and 12a/12b were separated by HPLC and the absolute configuration assigned by VCD technique in combination with ab initio Hartree-Fock calculations. Analogues 11a (RS-isomer) and 12b (RR-isomer) inhibited EAAT1 (IC(50) values 5.5 and 3.8 μM, respectively), whereas analogues 11b (SS-isomer) and 12a (SR-isomer) failed to inhibit EAAT1 uptake (IC(50) values >300 μM). PMID:22594609

  20. Line Positions and Intensities in the 2nu2/nu4 Vibrational System of 14NH3 near 5-7 micron

    NASA Astrophysics Data System (ADS)

    Cottaz, C.; Kleiner, I.; Tarrago, G.; Brown, L. R.; Margolis, J. S.; Poynter, R. L.; Pickett, H. M.; Fouchet, T.; Drossart, P.; Lellouch, E.

    2000-10-01

    Line positions and intensities belonging to the vibrational system 2nu2/nu4 of ammonia 14NH3 are measured and analyzed between 1200 and 2200 cm-1 in order to improve the molecular database. For this, laboratory spectra are obtained at 0.006 and 0.011 cm-1 unapodized resolution and with 4% precisions for the intensities using Fourier transform spectrometers located at the Kitt Peak National Observatory and the Jet Propulsion Laboratory. The observed data contain transitions of the nu4 fundamental band near 1626.276(1) and 1627.375(2) cm-1 (for s and a inversion upper states, respectively) and the 2nu2 overtone band near 1597.470(3) and 1882.179(5) cm-1 (for s and a inversion states, respectively). A total of 2345 lines with J'<=15 is assigned from which 2114 line positions with J'<=15 are fitted using an effective rotation-inversion-rotation Hamiltonian to achieve an rms of 0.003 cm-1 with 57 molecular parameters. Over 1200 intensity measurements are modeled to +/-4.7% using 16 terms of the dipole moment expansion. A dyad model is used in order to model all the interactions expected within the 2nu2/nu4 system. The bandstrength of 2nu2 (s <- a), 2nu2 (a <- s) and nu4 (s <- s and a <- a) are estimated to be 6.68(24), 0.201(5) and 116(3) cm-1 atm-1, respectively, at 296 K. The prediction generated by this study is available for planetary studies.

  1. Synthesis and Application of 1,3,4,5,7,8-Hexafluorotetracyanonaphthoquinodimethane (F6-TNAP): A Conductivity Dopant for Organic Light-Emitting Devices

    SciTech Connect

    Koech, Phillip K.; Padmaperuma, Asanga B.; Wang, Liang; Swensen, James S.; Polikarpov, Evgueni; Darsell, Jens T.; Rainbolt, James E.; Gaspar, Daniel J.

    2010-07-13

    We report the synthesis, photophysical and organic light-emitting device (OLED) properties of an organic molecular p-dopant 1,3,4,5,7,8-hexafluorotetracyanonaphthoquinodimethane (F6-TNAP). F6-TNAP was obtained in a three step 2 pot synthesis from commercially available octafluoronaphthalene. Doping effect of F6-TNAP was evaluated using films of 1-5% F6-TNAP with N,N'-di-1-naphthyl-N,N'-diphenyl-1,1'-biphenyl-4,4'diamineα-NPD) co-evaporated on quartz. UV-vis analysis of these films showed an absorption peak at 950 nm corresponding to the charge transfer complex resulting from electron transfer from α-NPD to F6-TNAP. Hole only devices using α-NPD as the hole transport layer (HTL) doped with F6-TNAP show greater than 2V decrease in operating voltage compared to the undoped device. A decrease in operating voltage was also demonstrated in blue OLED devices using F6-TNAP doped HTL, with a slight decrease in external quantum efficiency (EQE), thus resulting in a net improvement in power efficiency.

  2. Effects of 3,3',4,4',5-pentachlorobiphenyl and 2,3,7,8-tetrachlorodibenzo-p-dioxin injected into the yolks of double-crested cormorant (Phalacrocorax auritus) eggs prior to incubation

    USGS Publications Warehouse

    Powell, D.C.; Aulerich, R.J.; Meadows, J.C.; Tillitt, D.E.; Kelly, M.E.; Stromborg, K.L.; Melancon, M.J.; Fitzgerald, S.D.; Bursian, S.J.

    1998-01-01

    Double-crested cormorant (Phalacrocorax auritus) eggs were injected with either 3,3',4,4',5-pentachlorobiphenyl (polychlorinated biphenyl [PCB] 126; 70-698 ?g/kg egg) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 1.3-11.7 ?g/kg egg) prior to incubation. These compounds were injected into the yolks of cormorant eggs collected from incomplete clutches at isolated colonies on Lake Winnipegosis, Manitoba, Canada. Eggs were incubated for approximately 26 to 28 d. After hatching the brain, bursa, heart, liver, and spleen were dissected and weighed. Torsos were preserved in formalin for examination of the gonads. Median lethal doses (LD50s) calculated from mortality data at hatching were 177 and 4.0 ?g/kg egg for PCB 126 and TCDD, respectively. No significant differences were found in the incidence of developmental abnormalities in any of the treatment groups. Bursa weights were significantly less in the greatest (11.7 ?g/kg egg) TCDD group compared to the vehicle control group. Spleen weights were significantly less in the 349 ?g PCB 126/kg egg and the 5.4 and 11.7 ?g TCDD/kg egg groups when compared to the vehicle control group. No histological alterations of the gonads were found. Hepatic ethoxyresorufin-O-deethylase activity in all PCB 126 and TCDD dose groups was significantly greater compared to the control activity. The toxic equivalency factor for PCB 126 was 0.02

  3. Molecular cloning, characterization and expression analysis of Wnt4, Wnt5, Wnt6, Wnt7, Wnt10 and Wnt16 from Litopenaeus vannamei.

    PubMed

    Zhang, Shuang; Li, Chao-Zheng; Yang, Qi-Hui; Dong, Xiao-Hui; Chi, Shu-Yan; Liu, Hong-Yu; Shi, Li-Li; Tan, Bei-Ping

    2016-07-01

    The Wnt (Wg-type MMTV integration site) signaling represents as the negative regulator of virus-induced innate immune responses. Wnt genes act as ligands to activate the Wnt signaling. To know more about the information of Wnt genes in invertebrates, Litopenaeus vannamei Wnt genes (LvWnts) were identified and characterized. In this study, Six Wnt genes (LvWnt4, LvWnt5, LvWnt6, LvWnt7, LvWnt10 and LvWnt16) were obtained in L. vannamei. The complete cDNAs open reading frames (ORF) of LvWnt4, LvWnt5, LvWnt6, LvWnt7, LvWnt10 and LvWnt16 were 1077 bp, 1107 bp, 1350 bp, 1047 bp, 1509 bp and 1158 bp (GenBank accession no. KU169896, KU169897, KU169898, KU169899, KU169900 and KU169901), encoding 358, 368, 449, 348, 502 and 385 amino acid (aa) residues respectively. All the six members of LvWnts contain a Wnt1 domain, which is considered as an important feature of Wnt gene family. ClustalW analysis with amino acid sequences revealed that the proportion of identity with other species was more than 48% for all the LvWnts except LvWnt10 (36-41%). The phylogenetic relationship analysis illustrated that different subtype of Wnts formed their own separate branches and were placed in branch of invertebrates respectively with strong bootstrap support. The constitutive expressions of LvWnts were confirmed by RT-PCR in all the examined five developmental stages and eleven tissues of L. vannamei with different express patterns. LvWnt4, LvWnt5 and LvWnt10 were expressed highest in nerve while LvWnt6, LvWnt7 and LvWnt16 were expressed highest in intestine, stomach and gill, respectively. In addition, all the LvWnts were regulated by white spot syndrome virus (WSSV) challenges at different levels in hepatopancreas, gill and hemocytes, suggesting that Wnt genes may play a role in the defense against pathogenic virus infection in innate immune of L. vannamei. PMID:27153750

  4. Aftershock relocation and frequency-size distribution, stress inversion and seismotectonic setting of the 7 August 2013 M = 5.4 earthquake in Kallidromon Mountain, central Greece

    NASA Astrophysics Data System (ADS)

    Ganas, Athanassios; Karastathis, Vassilios; Moshou, Alexandra; Valkaniotis, Sotirios; Mouzakiotis, Evangelos; Papathanassiou, George

    2014-03-01

    On August 7, 2013 a moderate earthquake (NOA ML = 5.1, NOA Mw = 5.4) occurred in central Kallidromon Mountain, in the Pthiotis region of central Greece. 2270 aftershocks were relocated using a modified 1-D velocity model for this area. The b-value of the aftershock sequence was b = 0.85 for a completeness magnitude of Mc = 1.7. The rate of aftershock decay was determined at p = 0.63. The spatial distribution of the aftershock sequence points towards the reactivation of a N70° ± 10°E striking normal fault at crustal depths between 8 and 13 km. A NNW-SSE cross-section imaged the activation of a steep, south dipping normal fault. A stress inversion analysis of 12 focal mechanisms showed that the minimum horizontal stress is extensional at N173°E. No primary surface ruptures were observed in the field; however, the earthquake caused severe damage in the villages of the Kallidromon area. The imaged fault strike and the orientation of the long-axis of the aftershock sequence distribution are both at a high-angle to the strike of known active faults in this area of central Greece. We interpret the Kallidromon seismic sequence as release of extensional seismic strain on secondary, steep faults inside the Fokida-Viotia crustal block.

  5. Synthesis, characterization, crystal structures and in vitro antistaphylococcal activity of organotin(IV) derivatives with 5,7-disubstituted-1,2,4-triazolo[1,5-a]pyrimidine.

    PubMed

    Girasolo, Maria Assunta; Canfora, Loredana; Sabatino, Piera; Schillaci, Domenico; Foresti, Elisabetta; Rubino, Simona; Ruisi, Giuseppe; Stocco, Giancarlo

    2012-01-01

    New organotin(IV) complexes of 5,7-ditertbutyl-1,2,4-triazolo[1,5-a]pyrimidine (dbtp) and 5,7-diphenyl-1,2,4-triazolo[1,5-a]pyrimidine (dptp) with 1:1 and/or 1:2 stoichiometry were synthesized and investigated by X-ray diffraction, FT-IR and (119)Sn Mössbauer in the solid state and by (1)H and (13)C NMR spectroscopy, in solution. Moreover, the crystal and molecular structures of Et(2)SnCl(2)(dbtp)(2) and Ph(2)SnCl(2)(EtOH)(2)(dptp)(2) are reported. The complexes contain hexacoordinated tin atoms: in Et(2)SnCl(2)(dbtp)(2) two 5,7-ditertbutyl-1,2,4-triazolo[1,5-a]pyrimidine molecules coordinate classically the tin atom through N(3) atom and the coordination around the tin atom shows a skew trapezoidal structure with axial ethyl groups. In Ph(2)SnCl(2)(EtOH)(2)(dptp)(2) two ethanol molecules coordinate tin through the oxygen atom and the 5,7-diphenyl-1,2,4-triazolo[1,5-a]pyrimidine molecules are not directly bound to the metal center but strictly H-bonded, through N(3), to the OH group of the ethanol moieties; Ph(2)SnCl(2)(EtOH)(2)(dptp)(2) has an all-trans structure and the C-Sn-C fragment is linear. On the basis of Mössbauer data, the 1:2 diorganotin(IV) complexes are advanced to have the same structure of Et(2)SnCl(2)(dbtp)(2), while Me(2)SnCl(2)(dptp)(2) to have a regular all-trans octahedral structure. A distorted cis-R(2) trigonal bipyramidal structure is assigned to 1:1 diorganotin(IV) complexes. The in vitro antibacterial activities of the synthesized complexes have been tested against a group of reference pathogen micro-organisms and some of them resulted active with MIC values of 5μg/mL, most of all against staphylococcal strains, which shows their inhibitory effect. PMID:22119808

  6. 5-Demethylnobiletin and 5-Acetoxy-6,7,8,3',4'-pentamethoxyflavone Suppress Lipid Accumulation by Activating the LKB1-AMPK Pathway in 3T3-L1 Preadipocytes and High Fat Diet-Fed C57BL/6 Mice.

    PubMed

    Tung, Yen-Chen; Li, Shiming; Huang, Qingrong; Hung, Wei-Lun; Ho, Chi-Tang; Wei, Guor-Jien; Pan, Min-Hsiung

    2016-04-27

    Polymethoxyflavones (PMFs) and hydroxylated polymethoxyflavones (HPMFs), such as nobiletin (Nob) and 5-demethylnobiletin (5-OH-Nob), are unique flavonoids that are found exclusively in citrus peels. Nobiletin has been shown to suppress adipogenesis in vitro, but the antiadipogenic activity of 5-OH-Nob has not been investigated. Both nobiletin and 5-OH-Nob have poor aqueous solubility and low oral bioavailability. We employed chemical modification to produce the acetyl derivative of 5-OH-Nob, that is, 5-acetyloxy-6,7,8,3',4'-pentamethoxyflavone (5-Ac-Nob), to improve its bioavailability and bioactive efficiency. We found that 5-Ac-Nob reduced triacylglycerol (TG) content to a greater extent than 5-OH-Nob in 3T3-L1 preadipocytes. Orally administered 5-Ac-Nob resulted in a significant reduction in body weight, intra-abdominal fat, plasma and liver TG levels, and plasma cholesterol level in high fat diet-induced obese male C57BL/6J mice. The 5-Ac-Nob treatment decreased lipid accumulation by triggering the adenosine 5'-monophosphate-activated protein kinase (AMPK) pathway to alter transcriptional factors or lipogenesis-related enzymes in vivo and in vitro. PMID:27041493

  7. 4-Hydroxybenzyl modification of the highly teratogenic retinoid, 4-[(1E)-2-(5,5,8,8-tetramethyl-5,6,7,8-tetrahydro-2-naphthalenyl)-1-propen-1-yl]benzoic acid (TTNPB), yields a compound that induces apoptosis in breast cancer cells and shows reduced teratogenicity.

    PubMed

    Anding, Allyson L; Nieves, Nirca J; Abzianidze, Victoria V; Collins, Michael D; Curley, Robert W; Clagett-Dame, Margaret

    2011-11-21

    Retinoids are a class of compounds with structural similarity to vitamin A. These compounds inhibit the proliferation of many cancer cell lines but have had limited medical application as they are often toxic at therapeutic levels. Efforts to synthesize retinoids with a greater therapeutic index have met with limited success. 4-[(1E)-2-(5,5,8,8-tetramethyl-5,6,7,8-tetrahydro-2-naphthalenyl)-1-propen-1-yl]benzoic acid (TTNPB) is one of the most biologically active all-trans-retinoic acid (atRA) analogues and is highly teratogenic. In this study, we show that modification of the TTNPB carboxyl group with an N-(4-hydroxyphenyl)amido (4HPTTNPB) or a 4-hydroxybenzyl (4HBTTNPB) group changes the activity of the compound in cell culture and in vivo. Unlike TTNPB, both compounds induce apoptosis in cancer cells and bind poorly to the retinoic acid receptors (RARs). Like the similarly modified all-trans-retinoic acid (atRA) analogues N-(4-hydroxyphenyl)retinamide (4-HPR/fenretinide) and 4-hydroxybenzylretinone (4-HBR), 4HBTTNPB is a potent activator of components of the ER stress pathway. The amide-linked analogue, 4HPTTNPB, is less toxic to developing embryos than the parent TTNPB, and most significantly, the 4-hydroxybenzyl-modified compound (4HBTTNPB) that cannot be hydrolyzed in vivo to the parent TTNPB compound is nearly devoid of teratogenic liability. PMID:21939267

  8. A vibrational spectroscopic study of the silicate mineral lomonosovite Na5Ti2(Si2O7)(PO4)O2

    NASA Astrophysics Data System (ADS)

    Frost, Ray L.; López, Andrés; Theiss, Frederick L.; Graça, Leonardo M.; Scholz, Ricardo

    2015-01-01

    The mineral lomonosovite has been studied using a combination of scanning electron microscopy with energy dispersive X-ray analysis and vibrational spectroscopy. Qualitative chemical analysis gave Si, P, Na and Ti as the as major elements with small amounts of Mn, Ca, Fe and Al. The mineral lomonosovite has a formula Na5Ti2(Si2O7)(PO4)O2. Raman bands observed at 909, 925 and 939 cm-1 are associated with phosphate units. Raman bands found at 975, 999, 1070, 1080 and 1084 cm-1 are attributed to siloxane stretching vibrations. The observation of multiple bands in both the phosphate stretching and bending regions supports the concept that the symmetry of the phosphate anion in the structure of lomonosovite is significantly reduced. Infrared spectroscopy identifies bands in the water stretching and bending regions, thus suggesting that water is involved with the structure of lomonosovite either through adsorption on the surface or by bonding to the phosphate units.

  9. Polarization effects in rho/sup 0/-meson production in antiproton-proton interactions at 22. 4, 12, and 5. 7 GeV/c

    SciTech Connect

    Batyunya, B.V.; Boguslavsky, I.V.; Boos, E.G.; Dashian, N.B.; Dementiev, R.K.; Ermilova, D.I.; Gramenitsky, I.M.; Herynek, I.; Korzhavina, I.A.; Kuratashvili, G.O.

    1985-08-05

    The rho/sup 0/-meson spin alignment is studied in p-barp interactions at 22.4 and 12 GeV/c and in the reaction p-barp..-->..2..pi../sup +/2..pi../sup -/+neutrals at 5.7 GeV/c. An essential rho/sup 0/-meson spin alignment is observed. The values of the rho/sub 00//sup T/ element of the rho/sup 0/-meson spin-density matrix in the transversity frame are 0.56 +- 0.07, 0.53 +- 0.05, and 0.54 +- 0.04 for the above-mentioned interactions, respectively. An increase of rho/sub 00//sup T/ with rho/sup 0/ transverse momentum is obtained.

  10. Measurement of the neutron-capture cross section on 63,65Cu between 0.4 and 7.5 MeV

    NASA Astrophysics Data System (ADS)

    Bray, Isabel; Bhike, Megha; Krishichayan, (None); Tornow, W.

    2015-10-01

    Copper is currently being used as a cooling and shielding material in most experimental searches for 0 ν β β decay. In order to accurately interpret background events in these experiments, the cross section of neutron-induced reactions on copper must be known. The purpose of this work was to measure the cross section of the 63,65Cu(n, γ)64,66Cu reactions. Data were collected through the activation method at a range of energies from approximately 0.4 MeV to 7.5 MeV, employing the neutron production reactions 3H(p,n)3Heand2H(d,n)3He. Previous data were limited to energies below approximately 3 MeV. The results are compared to predictions from the nuclear data libraries ENDF/B-VII.1 and TENDL-2014.

  11. Molecular variants of human papilloma virus 16 E2, E4, E5, E6 and E7 genes associated with cervical neoplasia in Romanian patients.

    PubMed

    Plesa, Adriana; Anton, Gabriela; Iancu, Iulia V; Diaconu, Carmen C; Huica, Irina; Stanescu, Anca D; Socolov, Demetra; Nistor, Elena; Popa, Elena; Stoian, Mihai; Botezatu, Anca

    2014-12-01

    The aim of this study was to identify and associate the sequence variations of human Papillomavirus 16 (HPV16) genes from women who live in two different areas of Romania and associate them with malignant progression. One hundred twenty-four HPV16-positive cervical isolates were collected, and the E2, E4, E5, E6 and E7 viral genes were sequenced. Two new missense mutations in the E6 gene (C279G and A305C) were found (together or alone, in association with other mutations) in 44 of 124 cases. The most frequently simultaneously mutated genes were E4/E2 hinge, E5 and E6 (p = 0.0004) in squamous cell carcinoma (SCC) samples. Also, for SCC patients, the best-correlated mutation patterns were obtained for E4/E2 hinge-E5 (r = 0.7984; p < 0.0001). No sample was found to have all of the investigated viral genes concurrently mutated. Phylogenetic analysis was performed to characterize the viral variants. Similar results were found for SCC and cervical intraepithelial neoplasia III (CINIII) cases. After all of the target gene sequences were assembled, all patients were found to be infected with viruses of the HPV16- European-German (EG) lineage, and two clusters were identified, the first (55/96 variants) from Moldavia and the second (41/96 variants) from Bucharest. The distinct cluster derived from EG in Moldavia could partially explain the increased frequency of SCC in this area. This study has generated a comprehensive set of sequence variation data on HPV16 circulating in Romania to join the existing data and highlight the important role of HPV16 variants during cervical carcinogenesis. PMID:25143263

  12. The molecular structure of the borate mineral inderite Mg(H4B3O7)(OH)ṡ5H2O - A vibrational spectroscopic study

    NASA Astrophysics Data System (ADS)

    Frost, Ray L.; López, Andrés; Xi, Yunfei; Lima, Rosa Malena Fernandes; Scholz, Ricardo; Granja, Amanda

    2013-12-01

    We have undertaken a study of the mineral inderite Mg(H4B3O7)(OH)ṡ5H2O a hydrated hydroxy borate mineral of magnesium using scanning electron microscopy, thermogravimetry and vibrational spectroscopic techniques. The structure consists of [ soroborate groups and Mg(OH)2(H2O)4 octahedra interconnected into discrete molecules by the sharing of two OH groups. Thermogravimetry shows a mass loss of 47.2% at 137.5 °C, proving the mineral is thermally unstable. Raman bands at 954, 1047 and 1116 cm-1 are assigned to the trigonal symmetric stretching mode. The two bands at 880 and 916 cm-1 are attributed to the symmetric stretching mode of the tetrahedral boron. Both the Raman and infrared spectra of inderite show complexity. Raman bands are observed at 3052, 3233, 3330, 3392 attributed to water stretching vibrations and 3459 cm-1 with sharper bands at 3459, 3530 and 3562 cm-1 assigned to OH stretching vibrations. Vibrational spectroscopy is used to assess the molecular structure of inderite.

  13. Synthesis and Photoelectrochemistry Characterization of Polymer based on 4,7-Di(thiophen-2-yl)-benzo[c][1,2,5]thiadiazole, (DTBT)

    NASA Astrophysics Data System (ADS)

    Lazo Jimenez, Luz Maria; Frontana-Uribe, Bernardo Antonio

    Poly[4,7-di-(thiophen-2-yl)-benzo[c]-[1,2,5] thiadiazole], P(DTBT), is used in polymer:PCMB blends as active layer on organic photovoltaic devices, (OPV); DTBT-based copolymers show well-reversible oxidation and reduction electrochemical processes. These processes indicate their hig electrochemical stability suitable for n- and p-doping. This is a typical feature benzothiadiazole containing molecules. In the present study the synthesis conditions of the monomer, 4,7-di-(thiophen-2-yl)-benzo[c]-[1,2,5]-thiadiazole based on Stille coupling reactions has been investigated and its respectively polymer P(DTBT) was prepared by repetitive potential-sweep anodic oxidation of the corresponding monomer DTBT onto Pt disk or indium tin oxide (ITO) electrodes. Electrochemical cyclic voltammetry (CV) was performed to determine the HOMO and the LUMO energy levels of the conjugated DTBT and P(DTBT), both exhibit amphoteric redox properties, n- and p- doping process. The optical gap estimated from electrochemical measurements of the polymer P(DTBT) was found to be 1.77 eV, which is close to the reported band gap (1.1-1.2eV) determined by optical absorption technique . Photoelectrochemical characterization of P(DTBT) was realized from UV-Vis-NIR spectra recorded at different applied potentials. These result are correlated with the charge-transfer phenomena in the polymers applied as active layer on OPV`s. Av. Universidad 3000. Coyoacán.C.P. 04510. México. D.F. MEXICO.

  14. Oxidative Dearomatization of 4,5,6,7-Tetrahydro-1H-indoles Obtained by Metal- and Solvent-Free Thermal 5-endo-dig Cyclization: The Route to Erythrina and Lycorine Alkaloids.

    PubMed

    Andreev, Ivan A; Ratmanova, Nina K; Novoselov, Anton M; Belov, Dmitry S; Seregina, Irina F; Kurkin, Alexander V

    2016-05-17

    A facile one-pot approach based on a thermally induced metal- and solvent-free 5-endo-dig cyclization reaction of the amino propargylic alcohols in combination with Dess-Martin periodinane-promoted oxidative dearomatization of 4,5,6,7-tetrahydroindole intermediates provides an efficient and robust access to 5,6-dihydro-1H-indol-2(4H)ones. Green, relatively mild and operationally simple characteristics of the synthetic sequence are the major advantages, which greatly amplify the developed methodology. The utility of obtained indolones as unified key precursors is demonstrated by the application of these products to the formal total syntheses of a whole pleiad of Erythrina- and Lycorine-type alkaloids, namely (±)-erysotramidine, (±)-erysotrine, (±)-erythravine, (±)-γ-lycorane, and abnormal erythrinanes (±)-coccoline and (±)-coccuvinine. PMID:27076115

  15. Lead optimization of a pyrazolo[1,5-a]pyrimidin-7(4H)-one scaffold to identify potent, selective and orally bioavailable KDM5 inhibitors suitable for in vivo biological studies.

    PubMed

    Liang, Jun; Zhang, Birong; Labadie, Sharada; Ortwine, Daniel F; Vinogradova, Maia; Kiefer, James R; Gehling, Victor S; Harmange, Jean-Christophe; Cummings, Richard; Lai, Tommy; Liao, Jiangpeng; Zheng, Xiaoping; Liu, Yichin; Gustafson, Amy; Van der Porten, Erica; Mao, Weifeng; Liederer, Bianca M; Deshmukh, Gauri; Classon, Marie; Trojer, Patrick; Dragovich, Peter S; Murray, Lesley

    2016-08-15

    Starting with a lead [1,5-a]pyrimidin-7(4H)-one-containing molecule (1), we generated potent, selective and orally bioavailable KDM5 inhibitors. Using structure- and property-based approaches, we designed 48 with improved cell potency (PC9 H3K4Me3 EC50=0.34μM). Furthermore, 48 maintained suitable physiochemical properties and displayed an excellent pharmacokinetic (PK) profile in mice. When dosed orally in mice at 50mg/kg twice a day (BID), 48 showed an unbound maximal plasma concentration (Cmax) >15-fold over its cell EC50, thereby providing a robust chemical probe for studying KDM5 biological functions in vivo. PMID:27406798

  16. Second order phase transition temperature of single crystals of Gd5Si1.3Ge2.7 and Gd5Si1.4Ge2.6

    DOE PAGESBeta

    Hadimani, R. L.; Melikhov, Y.; Schlagel, D. L.; Lograsso, T. A.; Dennis, K. W.; McCallum, R. W.; Jiles, D. C.

    2015-01-30

    Gd5(SixGe1–x)4 has mixed phases in the composition range 0.32 < x < 0.41, which have not been widely studied. In this paper, we have synthesized and indexed single crystal samples of Gd5Si1.3Ge2.7 and Gd5Si1.4Ge2.6. In this study, we have investigated the first order and second order phase transition temperatures of these samples using magnetic moment vs. temperature and magnetic moment vs. magnetic field at different temperatures. We have used a modified Arrott plot technique that was developed and reported by us previously to determine the “hidden” second order phase transition temperature of the orthorhombic II phase.

  17. 9,9-Dioctyl-2,7-bis­(4,4,5,5-tetra­methyl-1,3,2-dioxaborolan-2-yl)-9H-fluorene

    PubMed Central

    Gagnon, Eric; Laliberté, Dominic

    2008-01-01

    In the title mol­ecule, C41H64B2O4, the fluorene unit is essentially planar and the two octyl chains attached to the central C atom inhibit the mol­ecule from engaging in inter­molecular aromatic inter­actions. One of the octyl chains adopts a fully extended conformation, whereas the second incorporates a single gauche conformation. Of the two pinacolatoboronate groups attached at the 2,7-positions, one is partly disordered; one ring C atom and all four methyl groups are disordered equally over two positions. PMID:21203296

  18. Protective effects of 5,7,4'-trihydroxy-6,3'dimethoxy-flavone 5-O-α-l-rhamnopyranoside, isolated from Annona squamosa leaves in thyrotoxicosis and in hepatic lipid peroxidation in rats.

    PubMed

    Panda, Sunanda; Kar, Anand

    2015-12-15

    Hitherto unknown protective effects of 5,7,4'-trihydroxy-6,3'dimethoxy-flavone 5-O-α-l-rhamnopyranoside (THDMF-Rha); isolated from Annona squamosa leaves were evaluated in l-thyroxine (l-T4)-induced thyrotoxicosis in rats. Administration of l-T4 at 500μg/kg body weight for 12days increased the levels of serum thyroid hormones, the activity of 5'-monodeiodinase-I (5'DI) and hepatic glucose-6-phosphatase (G-6Pase) as well as lipid peroxidation (LPO); with a parallel decrease in the levels of cellular antioxidants and serum lipids. However, administration of the isolated THDMF-Rha at a pre-standardized dose for 15days ameliorated the l-T4-induced alterations in the levels of thyroid hormones, hepatic LPO, G-6-Pase, 5'DI activity, and cellular levels of antioxidants and improved the status of different serum lipids, suggesting its antithyroidal and antioxidative potential. As compared to standard antithyroid drug, propylthiouracil, THDMF-Rha appeared to be more promising. PMID:26547692

  19. The arylpiperazine derivatives N-(4-cyanophenylmethyl)-4-(2-diphenyl)-1-piperazinehexanamide and N-benzyl-4-(2-diphenyl)-1-piperazinehexanamide exert a long-lasting inhibition of human serotonin 5-HT7 receptor binding and cAMP signaling.

    PubMed

    Atanes, Patricio; Lacivita, Enza; Rodríguez, Javier; Brea, José; Burgueño, Javier; Vela, José Miguel; Cadavid, María Isabel; Loza, María Isabel; Leopoldo, Marcello; Castro, Marián

    2013-12-01

    We performed a detailed in vitro pharmacological characterization of two arylpiperazine derivatives, compound N-(4-cyanophenylmethyl)-4-(2-diphenyl)-1-piperazinehexanamide (LP-211) previously identified as a high-affinity brain penetrant ligand for 5-hydroxytryptamine (serotonin) type 7 (5-HT7) receptors, and its analog N-benzyl-4-(2-diphenyl)-1-piperazinehexanamide (MEL-9). Both ligands exhibited competitive displacement of [(3)H]-(2R)-1-[(3-hydroxyphenyl)sulfonyl]-2-[2-(4-methyl-1-piperidinyl)ethyl]pyrrolidine ([(3)H]-SB-269970) radioligand binding and insurmountable antagonism of 5-carboxamidotryptamine (5-CT)-stimulated cyclic adenosine monophosphate (cAMP) signaling in human embryonic kidney (HEK293) cells stably expressing human 5-HT7 receptors. They also inhibited forskolin-stimulated adenylate cyclase activity in 5-HT7-expressing HEK293 cells but not in the parental cell line. The compounds elicited long-lasting (at least 24 h) concentration-dependent inhibition of radioligand binding at 5-HT7-binding sites in whole-cell radioligand binding assays, after pretreatment of the cells with the compounds and subsequent compound removal. In cAMP assays, pretreatment of cells with the compounds rendered 5-HT7 receptors unresponsive to 5-CT and also rendered 5-HT7-expressing HEK293 cells unresponsive to forskolin. Compound 1-(2-biphenyl)piperazine (RA-7), a known active metabolite of LP-211 present in vivo, was able to partially inhibit 5-HT7 radioligand binding in a long-lasting irreversible manner. Hence, LP-211 and MEL-9 were identified as high-affinity long-acting inhibitors of human 5-HT7 receptor binding and function in cell lines. The detailed in vitro characterization of these two pharmacological tools targeting 5-HT7 receptors may benefit the study of 5-HT7 receptor function and it may lead to the development of novel selective pharmacological tools with defined functional properties at 5-HT7 receptors. PMID:25505568

  20. Characterization and cross calibration of Agfa D4, D7, and D8 and Kodak SR45 x-ray films against direct exposure film at 4.0-5.5 keV

    SciTech Connect

    Lanier, N.E.; Cowan, J.S.; Workman, J.

    2006-04-15

    Kodak direct exposure film (DEF) [B. L. Henke et al., J. Opt. Soc. Am. B 3, 1540 (1986)] has been the standard for moderate energy (1-10 keV) x-ray diagnostic applications among the high-energy-density and inertial confinement fusion research communities. However, market forces have prompted Kodak to discontinue production of DEF, leaving these specialized communities searching for a replacement. We have conducted cross-calibration experiments and film characterizations on five possible substitutes for Kodak DEF. The film types studied were Kodak's Biomax MR (BMR) and SR45 along with Agfa's D8, D7, and D4sc. None of the films tested matched the speed of DEF. BMR and D8 were closest but D8 exhibited lower noise, with superior resolution and dynamic range. Agfa D7, Agfa D4sc, and Kodak SR45 were significantly less sensitive than BMR and D8, however, the improvements they yielded in resolution and dynamic range warrant their use if experimental constraints allow.

  1. Characterization and cross calibration of Agfa D4, D7, and D8 and Kodak SR45 x-ray films against direct exposure film at 4.0-5.5 keV

    NASA Astrophysics Data System (ADS)

    Lanier, N. E.; Cowan, J. S.; Workman, J.

    2006-04-01

    Kodak direct exposure film (DEF) [B. L. Henke et al., J. Opt. Soc. Am. B 3, 1540 (1986)] has been the standard for moderate energy (1-10keV) x-ray diagnostic applications among the high-energy-density and inertial confinement fusion research communities. However, market forces have prompted Kodak to discontinue production of DEF, leaving these specialized communities searching for a replacement. We have conducted cross-calibration experiments and film characterizations on five possible substitutes for Kodak DEF. The film types studied were Kodak's Biomax MR (BMR) and SR45 along with Agfa's D8, D7, and D4sc. None of the films tested matched the speed of DEF. BMR and D8 were closest but D8 exhibited lower noise, with superior resolution and dynamic range. Agfa D7, Agfa D4sc, and Kodak SR45 were significantly less sensitive than BMR and D8, however, the improvements they yielded in resolution and dynamic range warrant their use if experimental constraints allow.

  2. Transmitted/Founder and Chronic Subtype C HIV-1 Use CD4 and CCR5 Receptors with Equal Efficiency and Are Not Inhibited by Blocking the Integrin α4β7

    PubMed Central

    Banks, Lauren B.; Iyer, Shilpa S.; Pfaff, Jennifer M.; Salazar-Gonzalez, Jesus F.; Salazar, Maria G.; Decker, Julie M.; Parrish, Erica H.; Berg, Anna; Hopper, Jennifer; Hora, Bhavna; Kumar, Amit; Mahlokozera, Tatenda; Yuan, Sally; Coleman, Charl; Vermeulen, Marion; Ding, Haitao; Ochsenbauer, Christina; Tilton, John C.; Permar, Sallie R.; Kappes, John C.; Betts, Michael R.; Busch, Michael P.; Gao, Feng; Montefiori, David; Haynes, Barton F.; Shaw, George M.; Hahn, Beatrice H.; Doms, Robert W.

    2012-01-01

    Sexual transmission of human immunodeficiency virus type 1 (HIV-1) most often results from productive infection by a single transmitted/founder (T/F) virus, indicating a stringent mucosal bottleneck. Understanding the viral traits that overcome this bottleneck could have important implications for HIV-1 vaccine design and other prevention strategies. Most T/F viruses use CCR5 to infect target cells and some encode envelope glycoproteins (Envs) that contain fewer potential N-linked glycosylation sites and shorter V1/V2 variable loops than Envs from chronic viruses. Moreover, it has been reported that the gp120 subunits of certain transmitted Envs bind to the gut-homing integrin α4β7, possibly enhancing virus entry and cell-to-cell spread. Here we sought to determine whether subtype C T/F viruses, which are responsible for the majority of new HIV-1 infections worldwide, share biological properties that increase their transmission fitness, including preferential α4β7 engagement. Using single genome amplification, we generated panels of both T/F (n = 20) and chronic (n = 20) Env constructs as well as full-length T/F (n = 6) and chronic (n = 4) infectious molecular clones (IMCs). We found that T/F and chronic control Envs were indistinguishable in the efficiency with which they used CD4 and CCR5. Both groups of Envs also exhibited the same CD4+ T cell subset tropism and showed similar sensitivity to neutralization by CD4 binding site (CD4bs) antibodies. Finally, saturating concentrations of anti-α4β7 antibodies failed to inhibit infection and replication of T/F as well as chronic control viruses, although the growth of the tissue culture-adapted strain SF162 was modestly impaired. These results indicate that the population bottleneck associated with mucosal HIV-1 acquisition is not due to the selection of T/F viruses that use α4β7, CD4 or CCR5 more efficiently. PMID:22693444

  3. LC-UV-Guided Isolation and Structure Determination of Lancolide E: A Nortriterpenoid with a Tetracyclo[5.4.0.0(2,4).0(3,7)]undecane-Bridged System from a "Talented" Schisandra Plant.

    PubMed

    Shi, Yi-Ming; Cai, Song-Liang; Li, Xiao-Nian; Liu, Miao; Shang, Shan-Zhai; Du, Xue; Xiao, Wei-Lie; Pu, Jian-Xin; Sun, Han-Dong

    2016-01-01

    Lancolide E (1) featuring a complex tetracyclo[5.4.0.0(2,4).0(3,7)]undecane-bridged system that is constructed by an eight-, a three-, and two five-membered carbon rings in a sterically congested region was obtained in trace amounts from a "talented" schinortriterpenoid producer Schisandra lancifolia. Its structure was fully characterized by combining 2D NMR spectroscopy, theoretical calculations, and X-ray diffraction analysis. The biogenetic pathway of 1 was proposed to involve a Prins cyclization. PMID:26673855

  4. Potency of 3,3{prime},4,4{prime},5-pentachlorobiphenyl (PCB 126), alone and in combination with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), to produce lake trout early life-stage mortality

    SciTech Connect

    Zabel, E.W.; Peterson, R.E.; Cook, P.M.

    1995-12-01

    Newly fertilized lake trout (Salvelinus namaycush) eggs were exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 3,3{prime},4,4{prime},5-pentachlorobiphenyl (PCB 126), or their combination, and sac fry mortality was used to determine toxic potencies. The toxic equivalency factor (TEF) for PCB 126 was 0.0030. The dose-response curve for the PCB 126/TCDD mixture based on TCDD toxic equivalents was not significantly different from that for TCDD alone, suggesting additivity between the two congeners in causing sac fry mortality.

  5. Synthesis and antitubercular activity of 7-(R)- and 7-(S)-methyl-2-nitro-6-(S)-(4-(trifluoromethoxy)benzyloxy)-6,7-dihydro-5H-imidazo[2,1-b][1,3]oxazines, analogues of PA-824

    PubMed Central

    Li, Xiaojin; Manjunatha, Ujjini H.; Goodwin, Michael B.; Knox, John E.; Lipinski, Christopher A.; Keller, Thomas H.; Barry, Clifton E.; Dowd, Cynthia S.

    2008-01-01

    Nitroimidazoles such as PA-824 and OPC-67683 are currently in clinical development as members of a promising new class of therapeutics for tuberculosis. While the antitubercular activity of these compounds is high, they both suffer from poor water solubility thus complicating development. We determined the single-crystal X-ray structure of PA-824 and found a close packing of the nitroimidazoles facilitated by a pseudoaxial conformation of the p-trifluoromethoxybenzyl ether. To attempt to disrupt this tight packing by destabilizing the axial preference of this side chain, we prepared the two diastereomers of the 7-methyl-nitroimidazo-oxazine. Determination of the crystal structure of the 7-(S)-methyl derivative (5, cis) revealed that the benzylic side chain remained pseudoaxial while the 7-(R)-methyl derivative (6, trans) adopted the desired pseudoequatorial conformation. Both derivatives displayed similar activities against Mycobacterium tuberculosis, but neither showed improved aqueous solubility, suggesting that inherent lattice stability is not likely to be a major factor in limiting solubility. Conformational analysis revealed that all three compounds have similar energetically accessible conformations in solution. Additionally, these results suggest that the nitroreductase that initially recognizes PA-824 is somewhat insensitive to substitutions at the 7-position. PMID:18358721

  6. Association of Brain DNA Methylation in SORL1, ABCA7, HLA-DRB5, SLC24A4, and BIN1 With Pathological Diagnosis of Alzheimer Disease

    PubMed Central

    Yu, Lei; Chibnik, Lori B.; Srivastava, Gyan P.; Pochet, Nathalie; Yang, Jingyun; Xu, Jishu; Kozubek, James; Obholzer, Nikolaus; Leurgans, Sue E.; Schneider, Julie A.; Meissner, Alexander; De Jager, Philip L.; Bennett, David A.

    2015-01-01

    IMPORTANCE Recent large-scale genome-wide association studies have discovered several genetic variants associated with Alzheimer disease (AD); however, the extent to which DNA methylation in these AD loci contributes to the disease susceptibility remains unknown. OBJECTIVE To examine the association of brain DNA methylation in 28 reported AD loci with AD pathologies. DESIGN, SETTING, AND PARTICIPANTS Ongoing community-based clinical pathological cohort studies of aging and dementia (the Religious Orders Study and the Rush Memory and Aging Project) among 740 autopsied participants 66.0 to 108.3 years old. EXPOSURES DNA methylation levels at individual CpG sites generated from dorsolateral prefrontal cortex tissue using a bead assay. MAIN OUTCOMES AND MEASURES Pathological diagnosis of AD by National Institute on Aging–Reagan criteria following a standard postmortem examination. RESULTS Overall, 447 participants (60.4%) met the criteria for pathological diagnosis of AD. Brain DNA methylation in SORL1, ABCA7, HLA-DRB5, SLC24A4, and BIN1 was associated with pathological AD. The association was robustly retained after replacing the binary trait of pathological AD with 2 quantitative and molecular specific hallmarks of AD, namely, Aβ load and paired helical filament tau tangle density. Furthermore, RNA expression of transcripts of SORL1 and ABCA7 was associated with paired helical filament tau tangle density, and the expression of BIN1 was associated with Aβ load. CONCLUSIONS AND RELEVANCE Brain DNA methylation in multiple AD loci is associated with AD pathologies. The results provide further evidence that disruption of DNA methylation is involved in the pathological process of AD. PMID:25365775

  7. Consistency of land surface reflectance data: presentation of a new tool and case study with Formosat-2, SPOT-4 and Landsat-5/7/8 data

    NASA Astrophysics Data System (ADS)

    Claverie, M.; Vermote, E.; Franch, B.; Huc, M.; Hagolle, O.; Masek, J.

    2013-12-01

    Maintaining consistent dataset of Surface Reflectance (SR) data derived from the large panel of in-orbit sensors is an important challenge to ensure long term analysis of earth observation data. Continuous validation of such SR products through comparison with a reference dataset is thus an important challenge. Validating with in situ or airborne SR data is not easy since the sensors rarely match completely the same spectral, spatial and directional characteristics of the satellite measurement. Inter-comparison between satellites sensors data appears as a valuable tool to maintain a long term consistency of the data. However, satellite data are acquired at various times of the day (i.e., variation of the atmosphere content) and within a relative large range of geometry (view and sun angles). Also, even if band-to-band spectral characteristics of optical sensors are closed, they rarely have identical spectral responses. As the results, direct comparisons without consideration of these differences are poorly suitable. In this study, we suggest a new systematic method to assess land optical SR data from high to medium resolution sensors. We used MODIS SR products (MO/YD09CMG) which benefit from a long term calibration/validation process, to assess SR from 3 sensors data: Formosat-2 (280 scenes 24x24km - 5 sites), SPOT-4 (62 scenes 120x60km - 1 site) and Landsat-5/7 (104 180x180km scenes - 50 sites). The main issue concerns the difference in term of geometry acquisition between MODIS and compared sensors data. We used the VJB model (Vermote et al. 2009, TGRS) to correct MODIS SR from BRDF effects and to simulate SR at the corresponding geometry (view and sun angles) of each pixel of the compared sensor data. The comparison is done at the CMG spatial resolution (0.05°) which ensures a constant field-of-view and negligible geometrical errors. Figure 1 displays the summary of the NIR results through APU graphs where metrics A, P and U stands for Accuracy, Precision and

  8. Molecularly imprinted poly(4-amino-5-hydroxy-2,7-naphthalenedisulfonic acid) modified glassy carbon electrode as an electrochemical theophylline sensor.

    PubMed

    Aswini, K K; Vinu Mohan, A M; Biju, V M

    2016-08-01

    Theophylline is an inexpensive drug employed in asthma and chronic obstructive pulmonary disorder medications and is toxic at higher concentration. The development of a molecularly imprinted polymer based theophylline electrochemical sensor on glassy carbon electrode by the electropolymerization of 4-amino-5-hydroxy-2,7-naphthalenedisulfonic acid is being discussed in this work. The MIP modification enhances the theophylline recognition ability and the electron transfer kinetics of the bare electrode. The parameters, controlling the performance of the imprinted polymer based sensor, like number of electropolymerization cycles, composition of the pre-polymerization mixture, pH and immersion time were investigated and optimized. The interaction energy and the most stable conformation of the template-monomer complex in the pre-polymerization mixture were determined computationally using ab initio calculations based on density functional theory. The amperometric measurements showed that the developed sensor has a method detection limit of 0.32μM for the dynamic range of 0.4 to 17μM, at optimized conditions. The transducer possesses appreciable selectivity in the presence of structurally similar interferents such as theobromine, caffeine and doxofylline. The developed sensor showed remarkable stability and reproducibility and was also successfully employed in theophylline detection from commercially available tablets. PMID:27157734

  9. Electroexcitation of the Roper resonance for 1.7 < Q**2 < 4.5 -GeV2 in vec-ep ---> en pi+

    SciTech Connect

    Aznauryan, Inna; Burkert, Volker; Kim, Wooyoung; Park, Kil; Adams, Gary; Amaryan, Moscov; Amaryan, Moskov; Ambrozewicz, Pawel; Anghinolfi, Marco; Asryan, Gegham; Avagyan, Harutyun; Bagdasaryan, H.; Baillie, Nathan; Ball, J.P.; Ball, Jacques; Baltzell, Nathan; Barrow, Steve; Batourine, V.; Battaglieri, Marco; Bedlinskiy, Ivan; Bektasoglu, Mehmet; Bellis, Matthew; Benmouna, Nawal; Berman, Barry; Biselli, Angela; Blaszczyk, Lukasz; Bonner, Billy; Bookwalter, Craig; Bouchigny, Sylvain; Boyarinov, Sergey; Bradford, Robert; Branford, Derek; Briscoe, Wilbert; Brooks, William; Bultmann, S.; Bueltmann, Stephen; Butuceanu, Cornel; Calarco, John; Careccia, Sharon; Carman, Daniel; Casey, Liam; Cazes, Antoine; Chen, Shifeng; Cheng, Lu; Cole, Philip; Collins, Patrick; Coltharp, Philip; Cords, Dieter; Corvisiero, Pietro; Crabb, Donald; Crede, Volker; Cummings, John; Dale, Daniel; Dashyan, Natalya; De Masi, Rita; De Vita, Raffaella; De Sanctis, Enzo; Degtiarenko, Pavel; Denizli, Haluk; Dennis, Lawrence; Deur, Alexandre; Dhamija, Seema; Dharmawardane, Kahanawita; Dhuga, Kalvir; Dickson, Richard; Djalali, Chaden; Dodge, Gail; Donnelly, J.; Doughty, David; Dugger, Michael; Dytman, Steven; Dzyubak, Oleksandr; Egiyan, Hovanes; Egiyan, Kim; Elfassi, Lamiaa; Elouadrhiri, Latifa; Eugenio, Paul; Fatemi, Renee; Fedotov, Gleb; Feldman, Gerald; Feuerbach, Robert; Forest, Tony; Fradi, Ahmed; Funsten, Herbert; Gabrielyan, Marianna; Garcon, Michel; Gavalian, Gagik; Gevorgyan, Nerses; Gilfoyle, Gerard; Giovanetti, Kevin; Girod, Francois-Xavier; Goetz, John; Gohn, Wesley; Golovach, Evgeny; Gonenc, Atilla; Gordon, Christopher; Gothe, Ralf; Graham, L.; Griffioen, Keith; Guidal, Michel; Guillo, Matthieu; Guler, Nevzat; Guo, Lei; Gyurjyan, Vardan; Hadjidakis, Cynthia; Hafidi, Kawtar; Hafnaoui, Khadija; Hakobyan, Hayk; Hakobyan, Rafael; Hanretty, Charles; Hardie, John; Hassall, Neil; Heddle, David; Hersman, F.; Hicks, Kenneth; Hleiqawi, Ishaq; Holtrop, Maurik; Hyde, Charles; Ilieva, Yordanka; Ireland, David; Ishkhanov, Boris; Isupov, Evgeny; Ito, Mark; Jenkins, David; Jo, Hyon-Suk; Johnstone, John; Joo, Kyungseon; Juengst, Henry; Kalantarians, Narbe; Keller, Dustin; Kellie, James; Khandaker, Mahbubul; Kim, Kui; Klein, Andreas; Klein, Andreas; Klimenko, Alexei; Kossov, Mikhail; Krahn, Zebulun; Kramer, Laird; Kubarovsky, Valery; Kuhn, Joachim; Kuhn, Sebastian; Kuleshov, Sergey; Kuznetsov, Viacheslav; Lachniet, Jeff; Laget, Jean; Langheinrich, Jorn; Lawrence, Dave; Lee, T.; Lima, Ana; Livingston, Kenneth; Lu, Haiyun; Lukashin, Konstantin; MacCormick, Marion; Markov, Nikolai; Mattione, Paul; McAleer, Simeon; McKinnon, Bryan; McNabb, John; Mecking, Bernhard; Mehrabyan, Surik; Melone, Joseph; Mestayer, Mac; Meyer, Curtis; Mibe, Tsutomu; Mikhaylov, Konstantin; Minehart, Ralph; Mirazita, Marco; Miskimen, Rory; Mokeev, Viktor; Morand, Ludyvine; Moreno, Brahim; Moriya, Kei; Morrow, Steven; Moteabbed, Maryam; Mueller, James; Munevar Espitia, Edwin; Mutchler, Gordon; Nadel-Turonski, Pawel; Nasseripour, Rakhsha; Niccolai, Silvia; Niculescu, Gabriel; Niculescu, Maria-Ioana; Niczyporuk, Bogdan; Niroula, Megh; Niyazov, Rustam; Nozar, Mina; O'Rielly, Grant; Osipenko, Mikhail; Ostrovidov, Alexander; Park, S.; Pasyuk, Evgueni; Paterson, Craig; Anefalos Pereira, S.; Philips, Sasha; Pierce, Jerome; Pivnyuk, Nikolay; Pocanic, Dinko; Pogorelko, Oleg; Polli, Ermanno; Popa, Iulian; Pozdnyakov, Sergey; Preedom, Barry; Price, John; Prok, Yelena; Protopopescu, Dan; Qin, Liming; Raue, Brian; Riccardi, Gregory; Ricco, Giovanni; Ripani, Marco; Ritchie, Barry; Rosner, Guenther; Rossi, Patrizia; Rowntree, David; Rubin, Philip; Sabatie, Franck; Saini, Mukesh; Salamanca, Julian; Salgado, Carlos; Santoro, Joseph; Sapunenko, Vladimir; Schott, Diane; Schumacher, Reinhard; Serov, Vladimir; Sharabian, Youri; Sharov, Dmitri; Shaw, J.; Shvedunov, Nikolay; Skabelin, Alexander; Smith, Elton; Smith, Lee; Sober, Daniel; Sokhan, Daria; Stavinskiy, Aleksey; Stepanyan, Samuel; Stepanyan, Stepan; Stokes, Burnham

    2008-10-01

    DOI: http://dx.doi.org/10.1103/PhysRevC.78.045209
    The helicity amplitudes of the electroexcitation of the Roper resonance are extracted for 1.7 < Q2 < 4.5 GeV2 from recent high precision JLab-CLAS cross section and longitudinally polarized beam asymmetry data for pi+ electroproduction on protons at W=1.15-1.69 GeV. The analysis is made using two approaches, dispersion relations and a unitary isobar model, which give consistent results. It is found that the transverse helicity amplitude A_{1/2} for the gamma* p -> N(1440)P11 transition, which is large and negative at Q2=0, becomes large and positive at Q2 ~ 2 GeV2, and then drops slowly with Q2. The longitudinal helicity amplitude S_{1/2}, which was previously found from CLAS ep -> eppi0,enpi+ data to be large and positive at Q2=0.4,0.65 GeV2, drops with Q2. Available model predictions for gamma* p -> N(1440)P11 allow us to conclude that these results provide strong evidence in favor of N(1440)P11 as a first radial excitation of

  10. Anti-inflammatory effect of the 5,7,4'-trihydroxy-6-geranylflavanone isolated from the fruit of Artocarpus communis in S100B-induced human monocytes.

    PubMed

    Lin, Jer-An; Fang, Song-Chwan; Wu, Chi-Hao; Huang, Shang-Ming; Yen, Gow-Chin

    2011-01-12

    The fruit of Artocarpus communis Moraceae, a traditional starch crop, is a rich source of phytochemicals, such as flavonoids and their derivatives. The aim of this study was to investigate whether 5,7,4'-trihydroxy-6-geranylflavanone (AC-GF), a geranyl flavonoid derivative isolated from the fruits of A. communis, could decrease the activation of inflammatory mediators induced by S100B (ligand of receptor for advanced glycation end products, RAGE) in THP-1 monocytes. According to the results, low levels of AC-GF (≤2.5 μM) showed a great inhibitory effect on gene expression of RAGE and down-regulated both TNF-α and IL-1β secretion and gene expression (p < 0.05). AC-GF also decreased reactive oxygen species (ROS) production in response to S100B (p < 0.05). Additionally, Western blotting revealed that AC-GF could effectively attenuate RAGE-dependent signaling, including expression of protein kinase C (PKC) and p47phox, phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK), and particularly NF-κB activation (p < 0.05). In conclusion, this is the first report that AC-GF possesses great antioxidant and anti-inflammatory properties in vitro. This finding may contribute to increased implication and utilization of the fruit of A. communis Moraceae in functional foods. PMID:21126004

  11. The Synthesis of Methyl-Substituted Spirocyclic Piperidine-Azetidine (2,7-Diazaspiro[3.5]nonane) and Spirocyclic Piperidine-Pyrrolidine (2,8-Diazaspiro[4.5]decane) Ring Systems.

    PubMed

    Smith, Aaron C; Cabral, Shawn; Kung, Daniel W; Rose, Colin R; Southers, James A; García-Irizarry, Carmen N; Damon, David B; Bagley, Scott W; Griffith, David A

    2016-05-01

    The synthesis of a series of pharmaceutically important N-protected methyl-substituted spirocyclic piperidine-azetidine (2,7-diazaspiro[3.5]nonane) and spirocyclic piperidine-pyrrolidine (2,8-diazaspiro[4.5]decane) ring systems was developed. These motifs contain two differentiated sites (protected secondary amines) to allow for further functionalization via reductive amination, amidation, or other chemistry. The methyl-substituted spiroazetidine ring systems were accessed using nitrile lithiation/alkylation chemistry while the methyl-substituted spiropyrrolidines were synthesized by 1,4-addition reactions with nitroalkanes, followed by reduction and cyclization. These conditions were then scaled for the synthesis of 1-methyl spirocyclic piperidine-pyrrolidine with a classical resolution of the product using a tartaric acid derivative to isolate a single enantiomer. PMID:27056793

  12. Synthesis and biological evaluation of novel 9-heteroaryl substituted 7-chloro-4,5-dihydro-4-oxo-1,2,4-triazolo[1,5-a]quinoxaline-2-carboxylates (TQX) as (R,S)-2-amino-3-(3-hydroxy-5-methylisoxazol-4-yl)propionic acid (AMPA) receptor antagonists.

    PubMed

    Catarzi, Daniela; Colotta, Vittoria; Varano, Flavia; Filacchioni, Guido; Gratteri, Paola; Sgrignani, Jacopo; Galli, Alessandro; Costagli, Chiara

    2008-08-01

    In this paper, we report a study on some new 4,5-dihydro-4-oxo-1,2,4-triazolo[1,5-a]quinoxaline-2-carboxylate derivatives (TQXs), bearing a nitrogen-containing heterocycle at position-9, and designed as (R,S)-2-amino-3-(3-hydroxy-5-methylisoxazol-4-yl)propionic acid (AMPA) receptor antagonists. These compounds ensue from the structural modification of previously reported 8-heteroaryl-TQXs which were endowed with high affinity and selectivity for the AMPA receptor. All the newly synthesized compounds were biologically evaluated for their binding at the AMPA receptor. Gly/N-methyl-D-aspartic acid (NMDA) and kainic acid (KA) high-affinity binding assays were performed to assess the selectivity of the reported derivatives toward the AMPA receptor. This study produced some new TQXs which are less potent than the reference compounds, and endowed with a mixed AMPA and Gly/NMDA receptor binding affinity. To rationalize the experimental findings, a molecular modeling study was performed by docking some TQX derivatives to the AMPA receptor model. PMID:18670107

  13. Localized ageing in the heat affected zone of welded X5CrNiCuNbl6-4 and X4CrNiSiTi14-7 sheets

    NASA Astrophysics Data System (ADS)

    Sakhawat, S.; Falahati, A.; Degischer, H. P.; Spiradek, K.; Dománková, M.

    2014-06-01

    Localized ageing and corresponding microstructural developments as a result of welding heat in the heat-affected zone (HAZ) of two different precipitation-hardened Cr-Steels (X5CrNiCuNb16-4 and X4CrNiSiTi14-7) have been studied. The X5CrNiCuNb16-4 sheet was in solution annealed condition and X4CrNiSiTi14-7 sheet was in peak aged condition. The results showed that despite of initial heat treated condition, the fusion zone formed in both welded sheets has typical cast structure. The HAZ has different microstructure compared to fusion zone and base metal. The HAZ is found to be sensitive to the welding heat and was aged locally due to thermal effects of welding. This localized ageing forms regions in HAZ varying from over-aged to under aged, depending upon the initial ageing condition of the base sheet.

  14. Benzene-1,2,4,5-tetra-carb-oxy-lic acid bis-(1,3,7-trimethyl-2,3,6,7-tetra-hydro-1H-purine-2,6-dione).

    PubMed

    Arman, Hadi D; Tiekink, Edward R T

    2013-01-01

    The asymmetric unit of the title co-crystal, C10H6O8·2C8H10N4O2, comprises a centrosymmetric benzene-1,2,4,5-tetra-carb-oxy-lic acid (LH4) mol-ecule and a mol-ecule of caffeine in a general position. LH4 is nonplanar, with the dihedral angles between the ring and pendent carb-oxy-lic acid groups being 44.22 (7) and 49.74 (7)°. By contrast, the caffeine mol-ecule is planar (r.m.s. deviation = 0.040 Å). Supra-molecular layers parallel to (-1-10) are sustained by carb-oxy-lic acid O-H⋯O(carbon-yl) and O-H⋯N(imidazole) hydrogen bonds, as well as by meth-yl-carbonyl C-H⋯O inter-actions. These stack via π-π inter-actions between the benzene and imidazole rings [inter-centroid distance = 3.4503 (10) Å]. PMID:24427071

  15. Spectroscopic and thermal studies on charge-transfer complexes of perhydroisoquinoline with p-chloranil, 2,3-dichloro-5,6-dicyano-1,4-benzoquinone, and 7,7',8,8'-tetracyanoquinodimethane

    NASA Astrophysics Data System (ADS)

    Refat, Moamen S.; Abou-Melha, Sraa

    2012-03-01

    Charge-transfer interactions of perhydroisoquinoline (PHIQ) with 2,3-dichloro-5,6-dicyano-1,4- benzoquinone (DDQ), p-chloranil (CHL), and 7,7',8,8'-tetracyanoquinodimethane (TCNQ) in chloroform as a solvent have resulted in stable complexes with a general formula [(PHIQ)(acceptor)] with a molar ratio of 1:1 (donor:acceptor). Elemental (C, H, N) and thermogravimetric (TGA/DTG) analyses, photometric titration, and electronic, infrared, and 1H NMR spectra were used to give an idea of the charge-transfer interaction between donating and accepting sites. The Benesi-Hildebrand method and its modification were used to determine an association constant (K) and a molar extinction coefficient (ɛ).

  16. 2,4,5-Trichlorophenol

    Integrated Risk Information System (IRIS)

    2,4,5 - Trichlorophenol ; CASRN 95 - 95 - 4 Human health assessment information on a chemical substance is included in the IRIS database only after a comprehensive review of toxicity data , as outlined in the IRIS assessment development process . Sections I ( Health Hazard Assessments for Noncarcino

  17. A computational study on 4,7-di(furan-2-yl)benzo[c][1,2,5]thiadiazole monomer and its oligomers.

    PubMed

    Kayi, Hakan

    2014-06-01

    The energy gap, Eg, between the highest occupied molecular orbital (HOMO) and the lowest unoccupied molecular orbital (LUMO) energy levels that determines the electronic and optical properties of 4,7-di(furan-2-yl)benzo[c][1,2,5]thiadiazole (FSF) polymer is calculated by performing quantum chemical calculations. First, we theoretically investigated the most stable conformers of FSF monomer and its corresponding oligomers at the B3LYP/6-31G(d) and B3LYP/LANL2DZ levels of theory. We reveal the theoretical molecular structure of this very recently synthesized novel monomer and its oligomers for the first time in the literature. Our results from the B3LYP/6-31G(d) calculations indicated that FSF polymer has a low HOMO-LUMO gap of 1.55 eV to be in good agreement with the experiments. Experimental design and synthesis of novel conjugated polymers require time-consuming and expensive procedures. The findings from this study are promising for the use of computational methods in the design of the novel conjugated polymers, and help to narrow the materials to be used in design and synthesis of conjugated polymers with desired properties. PMID:24881001

  18. 1,8-Di­aza­bicyclo­[5.4.0]undec-7-en-8-ium bromido­(phthalocyaninato)zincate

    PubMed Central

    Przybył, Bartosz; Janczak, Jan

    2014-01-01

    The title compound, (C9H17N2)[ZnBr(C32H16N8)], contains a bromido­(phthalocyaninato)zincate anion and a protonated 1,8-di­aza­bicyclo­[5.4.0]undece-7-ene cation, [DBUH]+. The central ZnII atom has a distorted square-pyramidal geometry, with four iso­indole N atoms of the macrocycle in equatorial positions and a bromide ion in the axial position. The latter has a relatively high displacement parameter, but no evidence for disorder was obtained. The central ZnII atom is displaced by 0.488 (3) Å from the mean plane defined by the four iso­indole N atoms. The [DBUH]+ cation is involved in an almost linear N—H⋯Br hydrogen bond. In the crystal, π–π inter­actions lead to a relatively short distance of 3.366 (3) Å between the phthalocyaninate rings. PMID:24940212

  19. Formation of a Vitamin C Conjugate of Acrolein and its Paraoxonase-mediated Conversion into 5,6,7,8-Tetrahydroxy-4-oxooctanal

    PubMed Central

    Kesinger, Nicholas G.; Langsdorf, Brandi L.; Yokochi, Alexandre F.; Miranda, Cristobal L.; Stevens, Jan F.

    2010-01-01

    Vitamin C (ascorbic acid) has been reported to participate in Michael addition reactions in vitro to form vitamin C conjugates with α,β-unsaturated aldehydes, such as acrolein. This study shows evidence for the formation and metabolism of the vitamin C conjugate of acrolein (AscACR) in cultured human monocytic THP-1 cells exposed to acrolein diacetate. By using 18O and 13C labeling in combination with liquid chromatography–tandem mass spectrometry, AscACR was shown to undergo hydrolytic conversion of the ascorbyl lactone into an intermediate carboxylic acid. Subsequent decarboxylation of the carboxylic acid yielded 5,6,7,8-tetrahydroxy-4-oxooctanal (THO). When THP-1 cells were pretreated with ascorbic acid (1 mM, 18 hours) and then exposed to acrolein diacetate, THO was detected as its pentafluorobenzyl oxime derivative in the cell lysates and medium. Treatment of THP-1 cells with both ascorbic acid and acrolein diacetate was required for THO formation. The formation of THO from AscACR was facilitated by the lactonase enzymes, human recombinant paraoxonases 1 and 2. THP-1 cells exhibited PON activity which explains the catalytic conversion of AscACR into THO in these cells. THO was formed in addition to metabolites of the glutathione conjugate of acrolein, indicating that THO formation contributes to the elimination of acrolein in a cellular environment. PMID:20353174

  20. Synthesis, characterization, and antimicrobial activity of silver carbene complexes derived from 4,5,6,7-tetrachlorobenzimidazole against antibiotic resistant bacteria†

    PubMed Central

    Wright, Brian D.; Shah, Parth N.; McDonald, Lucas J.; Shaeffer, Michael L.; Wagers, Patrick O.; Panzner, Matthew J.; Smolen, Justin; Tagaev, Jasur; Tessier, Claire A.

    2013-01-01

    Silver N-heterocyclic carbene complexes have been shown to have great potential as antimicrobial agents, affecting a wide spectrum of both Gram-positive and Gram-negative bacteria. A new series of three silver carbene complexes (SCCs) based on 4,5,6,7-tetrachlorobenzimidazole has been synthesized, characterized, and tested against a panel of clinical strains of bacteria. The imidazolium salts and their precursors were characterized by elemental analysis, mass spectrometry, 1H and 13C NMR spectroscopy, and single crystal X-ray diffraction. The silver carbene complexes, SCC32, SCC33, and SCC34 were characterized by elemental analysis, 1H and 13C NMR spectroscopy, and single crystal X-ray diffraction. These complexes proved highly efficacious with minimum inhibitory concentrations (MICs) ranging from 0.25 to 6 μg mL−1. Overall, the complexes were effective against highly resistant bacteria strains, such as methicillin-resistant Staphylococcus aureus (MRSA), weaponizable bacteria, such as Yersinia pestis, and pathogens found within the lungs of cystic fibrosis patients, such as Pseudomonas aeruginosa, Alcaligenes xylosoxidans, and Burkholderia gladioli. SCC33 and SCC34 also showed clinically relevant activity against a silver-resistant strain of Escherichia coli based on MIC testing. PMID:22402409

  1. Triplet energies and the singlet oxygen quenching mechanism for 7H-pyrazolo[5,1-c]-1,2,4-triazole azomethine dyes.

    PubMed

    Douglas, Peter; Thomas, Jonathan D; Strohm, Holger; Winscom, Chris; Clarke, Dave; Garley, Michael S

    2003-05-01

    Analysis of triplet energy transfer rate constants gives the triplet energies of six 7H-pyrazolo[5,1-c]-1,2,4-triazole azomethine dyes, with lambda max values in the range 546-633 nm in ethanol, to lie in the range 115-88 kJ mol-1. Energy transfer rates from porphyrin and phthalocyanine sensitisers can be well approximated using the Balzani equation with a zero or small reorganization energy, and a transmission coefficient ca. 1/1000 that of the fully adiabatic value. A comparison of data on triplet energies of azomethine dyes suggests a relationship between the dye absorption energies and triplet energies of the form: ET = 0.69(+/- 0.04)(E lambda max)-33(+/- 9) kJ mol-1. A detailed study of the quenching of 1O2* by one of the dyes shows that this reaction is accompanied by isomerisation of the dye. This is interpreted as strong indirect evidence for an energy transfer mechanism for the process, a conclusion which is supported in a general way by the value of the 1O2* quenching rate constant. PMID:12803079

  2. Crystal structure of 1,3-bis­(4-hexyl-5-iodo­thio­phen-2-yl)-4,5,6,7-tetra­hydro-2-benzo­thio­phene

    PubMed Central

    Linshoeft, Julian; Näther, Christian; Staubitz, Anne

    2014-01-01

    In the crystal structure of the title compound, C28H36I2S3, a terthio­phene monomer, the central thio­phene unit is arranged anti-coplanar to the two outer thio­phene rings. There are two crystallographically independent mol­ecules in the asymmetric unit, which show different conformations. In one mol­ecule, the dihedral angles between the inner and the two outer thiophene rings are 15.7 (3) and 3.47 (3)°, whereas these values are 4.2 (3) and 11.3 (3)° for the second mol­ecule. Differences are also found in the arrangement of the hexyl chains: in one of the two molecules, both chains are nearly in plane to the central moiety, whereas in the second molecule, only one chain is in plane and the other one is nearly perpendicular to the central moiety. Some of the C atoms are disordered and were refined using a split model with occupancy ratios of 0.65:0.35 and 0.70:0.30 in the two mol­ecules. PMID:25484713

  3. Quantum-chemical insight into structure-reactivity relationship in 4,5,6,7-tetrahalogeno-1H-benzimidazoles: a combined X-ray, DSC, DFT/QTAIM, Hirshfeld surface-based, and molecular docking approach.

    PubMed

    Latosińska, Jolanta Natalia; Latosińska, Magdalena; Maurin, Jan Krzysztof; Orzeszko, Andrzej; Kazimierczuk, Zygmunt

    2014-03-20

    The weak interaction patterns in 4,5,6,7-tetrahalogeno-1H-benzimidazoles, protein kinase CK2 inhibitors, in solid state are studied by the X-ray method and quantum chemistry calculations. The crystal structures of 4,5,6,7-tetrachloro- and 4,5,6,7-tetrabromo-1H-benzimidazole are determined by X-ray diffraction and refined to a final R-factor of 3.07 and 3.03%, respectively, at room temperature. The compound 4,5,6,7-tetrabromo-1H-benzimidazole, which crystallizes in the I41/a space group, is found to be isostructural with previously studied 4,5,6,7-tetraiodo-1H-benzimidazole in contrast to 4,5,6,7-tetrachloro-1H-benzimidazole, which crystallizes as triclinic P1̅ with 4 molecules in elementary unit. For 4,5,6,7-tetrachloro-1H-benzimidazole, differential scanning calorimetry (DSC) revealed a second order glassy phase transition at Tg = 95°/106° (heating/cooling), an indication of frozen disorder. The lack of 3D isostructurality found in all 4,5,6,7-tetrahalogeno-1H-benzimidazoles is elucidated on the basis of the intra- and intermolecular interactions (hydrogen bonding, van der Waals contacts, and C-H···π interactions). The topological Bader's Quantum Theory of Atoms in Molecules (QTAIM) and Spackman's Hirshfeld surface-based approaches reveal equilibration of electrostatic matching and dispersion van der Waals interactions between molecules consistent with the crystal site-symmetry. The weakening of van der Waals forces accompanied by increasing strength of the hydrogen bond (N-H···N) result in a decrease in the crystal site-symmetry and a change in molecular packing in the crystalline state. Crystal packing motifs were investigated with the aid of Hirshfeld surface fingerprint plots. The ordering 4,5,6,7-tetraiodo > 4,5,6,7-tetrabromo > 4,5,6,7-tetrachloro > 4,5,6,7-tetrafluoro reflects not only a decrease in crystal symmetry but also increase in chemical reactivity (electronic activation), which could explain some changes in biological activity of

  4. 10 CFR 960.5-2-7 - Transportation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Transportation. 960.5-2-7 Section 960.5-2-7 Energy... REPOSITORY Preclosure Guidelines Environment, Socioeconomics, and Transportation § 960.5-2-7 Transportation... using reasonably available technology; (iii) will not require transportation system components to...

  5. 10 CFR 960.5-2-7 - Transportation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Transportation. 960.5-2-7 Section 960.5-2-7 Energy... REPOSITORY Preclosure Guidelines Environment, Socioeconomics, and Transportation § 960.5-2-7 Transportation... using reasonably available technology; (iii) will not require transportation system components to...

  6. 10 CFR 960.5-2-7 - Transportation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Transportation. 960.5-2-7 Section 960.5-2-7 Energy... REPOSITORY Preclosure Guidelines Environment, Socioeconomics, and Transportation § 960.5-2-7 Transportation... using reasonably available technology; (iii) will not require transportation system components to...

  7. 10 CFR 960.5-2-7 - Transportation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Transportation. 960.5-2-7 Section 960.5-2-7 Energy... REPOSITORY Preclosure Guidelines Environment, Socioeconomics, and Transportation § 960.5-2-7 Transportation... using reasonably available technology; (iii) will not require transportation system components to...

  8. 10 CFR 960.5-2-7 - Transportation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Transportation. 960.5-2-7 Section 960.5-2-7 Energy... REPOSITORY Preclosure Guidelines Environment, Socioeconomics, and Transportation § 960.5-2-7 Transportation... using reasonably available technology; (iii) will not require transportation system components to...

  9. 4-(4-Bromo­phen­yl)-8-methyl-2-oxo-1,2,3,4,4a,5,6,7-octa­hydro­quinoline-3-carbonitrile

    PubMed Central

    Asiri, Abdullah M.; Faidallah, Hassan M.; Ng, Seik Weng; Tiekink, Edward R. T.

    2012-01-01

    In the title compound, C17H17BrN2O, the N-containing ring adopts an envelope conformation with the C atom carrying the phenyl ring displaced by −0.531 (9) Å from the plane defined by the remaining five atoms (r.m.s. deviation = 0.0099 Å). The benzene ring is almost orthogonal to the ring to which it is attached, the CCN—C—CPh—CPh torsion angle being −101.3 (7)°. The cyclo­hexene ring is disordered over two conformations in a statistical ratio. The most prominent inter­actions in the crystal are pairs of N—H⋯O hydrogen bonds between inversion-related mol­ecules. The resulting dimers are linked into a three-dimensional architecture by C—H⋯N, C—H⋯Br and C—H⋯π inter­actions. PMID:22904838

  10. 7 CFR 2502.5 - Program benefits and services.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... of 7 CFR part 3015, 7 CFR part 3016, 7 CFR part 3018, 7 CFR part 3019 and 7 CFR 3052. (b) The Office... of agricultural labor market information: (3) Transportation: (4) Short-term housing while in transit to an agricultural worksite; (5) Workplace literacy and assistance with English as a second...

  11. 7 CFR 2502.5 - Program benefits and services.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

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  12. 7 CFR 2502.5 - Program benefits and services.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

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  13. 40 CFR 721.9575 - Chromate(3-), bis[3-[[5-(aminosulfonyl)-2-hydroxyphenyl]azo]-4-hydroxy-7-[[2-oxo-1-[(phenylamino...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 32 2013-07-01 2013-07-01 false Chromate(3-), bis azo]-4-hydroxy-7... CHEMICAL SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.9575 Chromate(3-), bis azo... significant new uses subject to reporting. (1) The chemical substance identified as chromate(3-), bis...

  14. 40 CFR 721.9575 - Chromate(3-), bis[3-[[5-(aminosulfonyl)-2-hydroxyphenyl]azo]-4-hydroxy-7-[[2-oxo-1-[(phenylamino...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 30 2010-07-01 2010-07-01 false Chromate(3-), bis azo]-4-hydroxy-7... CHEMICAL SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.9575 Chromate(3-), bis azo... significant new uses subject to reporting. (1) The chemical substance identified as chromate(3-), bis...

  15. 40 CFR 721.9575 - Chromate(3-), bis[3-[[5-(aminosulfonyl)-2-hydroxyphenyl]azo]-4-hydroxy-7-[[2-oxo-1-[(phenylamino...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 31 2014-07-01 2014-07-01 false Chromate(3-), bis azo]-4-hydroxy-7... CHEMICAL SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.9575 Chromate(3-), bis azo... significant new uses subject to reporting. (1) The chemical substance identified as chromate(3-), bis...

  16. 40 CFR 721.9575 - Chromate(3-), bis[3-[[5-(aminosulfonyl)-2-hydroxyphenyl]azo]-4-hydroxy-7-[[2-oxo-1-[(phenylamino...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 31 2011-07-01 2011-07-01 false Chromate(3-), bis azo]-4-hydroxy-7... CHEMICAL SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.9575 Chromate(3-), bis azo... significant new uses subject to reporting. (1) The chemical substance identified as chromate(3-), bis...

  17. 40 CFR 721.9575 - Chromate(3-), bis[3-[[5-(aminosulfonyl)-2-hydroxyphenyl]azo]-4-hydroxy-7-[[2-oxo-1-[(phenylamino...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 32 2012-07-01 2012-07-01 false Chromate(3-), bis azo]-4-hydroxy-7... CHEMICAL SUBSTANCES Significant New Uses for Specific Chemical Substances § 721.9575 Chromate(3-), bis azo... significant new uses subject to reporting. (1) The chemical substance identified as chromate(3-), bis...

  18. Design, synthesis, biological evaluation and docking studies of novel 2-substituted-4-morpholino-7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidine derivatives as dual PI3Kα/mTOR inhibitors.

    PubMed

    Lei, Fei; Sun, Chengyu; Xu, Shan; Wang, Qinqin; OuYang, Yiqiang; Chen, Chen; Xia, Hui; Wang, Linxiao; Zheng, Pengwu; Zhu, Wufu

    2016-06-30

    Four series of 2-substituted-4-morpholino- 7,8-dihydro-5H-thiopyrano[4,3-d]pyrimidine derivatives (9-28) were designed, synthesized and their structures were confirmed by (1)H NMR, (13)C NMR and MS spectrum. All compounds were evaluated for the IC50 values against three cancer cell lines (A549, PC-3 and MCF-7). And four selected compounds (10, 11, 24, 27) were further evaluated for the IC50 values against PI3Kα and mTOR kinases. Seven of the target compounds exhibited moderate to excellent antitumor activities against these three cancer cell lines. The most promising compound 11 showed good antitumor potency for A549, PC-3 and MCF-7 cell lines with IC50 values of 0.52 ± 0.10 μM, 1.41 ± 0.10 μM, 4.82 ± 0.24 μM and moderate antitumor activities against PI3Kα/mTOR with IC50 values of 6.72 ± 0.30 μM and 0.94 ± 0.10 μM. Structure-activity relationships (SARs) and docking studies indicated that aryl urea scaffolds had a significant impact on the antitumor activities, and aryl pyridine urea scaffolds produced the best potency. Variations in substitutions of the aryl group had a significant impact on the activity and 3-Cl-4-F or 3-CF3-4-Cl substitution was more preferred. PMID:27043268

  19. Bis(2-amino-5-benzyl-3-eth­oxy­carbonyl-4,5,6,7-tetra­hydro­thieno[3,2-c]pyridin-5-ium) bis­(4-meth­oxy­phen­yl)di­phos­phon­ate

    PubMed Central

    Akkurt, Mehmet; Mague, Joel T.; Mohamed, Shaaban K.; Younes, Sabry H. H.; Albayati, Mustafa R.

    2014-01-01

    The asymmetric unit of the title salt, 2C17H21N2O2S+·C14H14O7P2 2−, contains half of a centrosymmetric bis­(4-meth­oxy­phen­yl)di­phospho­nate anion and one 2-amino-5-benzyl-3-eth­oxy­carbonyl-4,5,6,7-tetra­hydro­thieno[3,2-c]pyri­din-5-ium cation. In the anion, the O atoms of the di­phospho­nate group are disordered over two positions with equal occupancies. In the cation, the ethyl group is disordered over two orientations with a refined occupancy ratio of 0.753 (5):0.247 (5), and the tetra­hydro­pyridinium ring adopts a distorted half-chair conformation. In the crystal, the ions are linked by C—H⋯O, N—H⋯O and C—H⋯S hydrogen bonds into a three-dimensional network. PMID:24765038

  20. (2R*,4R*,7S*,10R*,12R*)-3,11,13,15-Tetra­oxa­penta­cyclo­[5.5.3.01,7.02,4.010,12]penta­deca-5,8-dien-14-one

    PubMed Central

    Mehta, Goverdhan; Sen, Saikat; Kumar, C. S. Ananda

    2013-01-01

    The title compound, C11H8O5, features a ‘skipped’ diene, an anti-bis­(epoxide) and a cyclic carbonate, all embedded in a densely functionalized [4.4.3]propellane scaffold. The crystal packing of this diepoxide is effected primarily by C—H⋯O hydrogen bonds, which link the mol­ecules into tapes along the b axis. Inter-tape connectivity is brought about by centrosymmetrically disposed pairs of C⋯O contacts [3.183 (4) Å] between the Cδ+=Oδ- dipoles of neighbouring carbonate moieties. PMID:24098200

  1. 6 CFR 5.7 - Classified information.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Freedom of Information Act § 5.7 Classified information. In processing a request for information that is classified under Executive Order 12958 (3 CFR, 1996 Comp., p. 333) or any other executive order, the... 6 Domestic Security 1 2011-01-01 2011-01-01 false Classified information. 5.7 Section 5.7...

  2. 6 CFR 5.7 - Classified information.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Freedom of Information Act § 5.7 Classified information. In processing a request for information that is classified under Executive Order 12958 (3 CFR, 1996 Comp., p. 333) or any other executive order, the... 6 Domestic Security 1 2014-01-01 2014-01-01 false Classified information. 5.7 Section 5.7...

  3. 6 CFR 5.7 - Classified information.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Freedom of Information Act § 5.7 Classified information. In processing a request for information that is classified under Executive Order 12958 (3 CFR, 1996 Comp., p. 333) or any other executive order, the... 6 Domestic Security 1 2013-01-01 2013-01-01 false Classified information. 5.7 Section 5.7...

  4. 6 CFR 5.7 - Classified information.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Freedom of Information Act § 5.7 Classified information. In processing a request for information that is classified under Executive Order 12958 (3 CFR, 1996 Comp., p. 333) or any other executive order, the... 6 Domestic Security 1 2012-01-01 2012-01-01 false Classified information. 5.7 Section 5.7...

  5. 6 CFR 5.7 - Classified information.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Freedom of Information Act § 5.7 Classified information. In processing a request for information that is classified under Executive Order 12958 (3 CFR, 1996 Comp., p. 333) or any other executive order, the... 6 Domestic Security 1 2010-01-01 2010-01-01 false Classified information. 5.7 Section 5.7...

  6. 22 CFR 19.7-5 - Limitations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Limitations. 19.7-5 Section 19.7-5 Foreign Relations DEPARTMENT OF STATE PERSONNEL BENEFITS FOR SPOUSES AND FORMER SPOUSES OF PARTICIPANTS IN THE FOREIGN SERVICE RETIREMENT AND DISABILITY SYSTEM § 19.7-5 Limitations. (a) A spousal agreement may...

  7. 22 CFR 19.7-5 - Limitations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Limitations. 19.7-5 Section 19.7-5 Foreign Relations DEPARTMENT OF STATE PERSONNEL BENEFITS FOR SPOUSES AND FORMER SPOUSES OF PARTICIPANTS IN THE FOREIGN SERVICE RETIREMENT AND DISABILITY SYSTEM § 19.7-5 Limitations. (a) A spousal agreement may...

  8. 15 CFR 5.7 - Reports.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Reports. 5.7 Section 5.7 Commerce and Foreign Trade Office of the Secretary of Commerce OPERATION OF VENDING STANDS § 5.7 Reports. No later than... forward to the Director, Office of Administrative Services a report on activities under this order....

  9. 15 CFR 5.7 - Reports.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Reports. 5.7 Section 5.7 Commerce and Foreign Trade Office of the Secretary of Commerce OPERATION OF VENDING STANDS § 5.7 Reports. No later than... forward to the Director, Office of Administrative Services a report on activities under this order....

  10. 42 CFR 7.5 - Payment procedures.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Payment procedures. 7.5 Section 7.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS DISTRIBUTION OF REFERENCE BIOLOGICAL STANDARDS AND BIOLOGICAL PREPARATIONS § 7.5 Payment procedures. The requester...

  11. 42 CFR 7.5 - Payment procedures.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Payment procedures. 7.5 Section 7.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS DISTRIBUTION OF REFERENCE BIOLOGICAL STANDARDS AND BIOLOGICAL PREPARATIONS § 7.5 Payment procedures. The requester...

  12. 42 CFR 7.5 - Payment procedures.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false Payment procedures. 7.5 Section 7.5 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS DISTRIBUTION OF REFERENCE BIOLOGICAL STANDARDS AND BIOLOGICAL PREPARATIONS § 7.5 Payment procedures. The requester...

  13. 7 CFR 772.5 - Security maintenance.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... liquidation if warranted. (3) For real estate security for IMP loans, service the account according to 7 CFR... 7 Agriculture 7 2010-01-01 2010-01-01 false Security maintenance. 772.5 Section 772.5 Agriculture... SPECIAL PROGRAMS SERVICING MINOR PROGRAM LOANS § 772.5 Security maintenance. (a) General. Borrowers...

  14. 7 CFR 772.5 - Security maintenance.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... liquidation if warranted. (3) For real estate security for IMP loans, service the account according to 7 CFR... 7 Agriculture 7 2012-01-01 2012-01-01 false Security maintenance. 772.5 Section 772.5 Agriculture... SPECIAL PROGRAMS SERVICING MINOR PROGRAM LOANS § 772.5 Security maintenance. (a) General. Borrowers...

  15. 47 CFR 7.5 - General Obligations.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 1 2014-10-01 2014-10-01 false General Obligations. 7.5 Section 7.5 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL ACCESS TO VOICEMAIL AND INTERACTIVE MENU SERVICES AND EQUIPMENT BY PEOPLE WITH DISABILITIES Obligations-What Must Covered Entities Do? § 7.5 General...

  16. 47 CFR 7.5 - General Obligations.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false General Obligations. 7.5 Section 7.5 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL ACCESS TO VOICEMAIL AND INTERACTIVE MENU SERVICES AND EQUIPMENT BY PEOPLE WITH DISABILITIES Obligations-What Must Covered Entities Do? § 7.5 General...

  17. 47 CFR 7.5 - General Obligations.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 47 Telecommunication 1 2013-10-01 2013-10-01 false General Obligations. 7.5 Section 7.5 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL ACCESS TO VOICEMAIL AND INTERACTIVE MENU SERVICES AND EQUIPMENT BY PEOPLE WITH DISABILITIES Obligations-What Must Covered Entities Do? § 7.5 General...

  18. 47 CFR 7.5 - General Obligations.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 47 Telecommunication 1 2012-10-01 2012-10-01 false General Obligations. 7.5 Section 7.5 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL ACCESS TO VOICEMAIL AND INTERACTIVE MENU SERVICES AND EQUIPMENT BY PEOPLE WITH DISABILITIES Obligations-What Must Covered Entities Do? § 7.5 General...

  19. 47 CFR 7.5 - General Obligations.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 47 Telecommunication 1 2011-10-01 2011-10-01 false General Obligations. 7.5 Section 7.5 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL ACCESS TO VOICEMAIL AND INTERACTIVE MENU SERVICES AND EQUIPMENT BY PEOPLE WITH DISABILITIES Obligations-What Must Covered Entities Do? § 7.5 General...

  20. Effect of the external field on the soft magnetic properties and microstructure of directly cast Fe75P8.7B5C7Si4.3 nanocrystalline sheets

    NASA Astrophysics Data System (ADS)

    Shih, C. W.; Lin, Y. Y.; Chang, H. W.; Lee, Y. I.; Chang, W. C.; Yang, C. C.

    2015-05-01

    A new method to fabricate soft magnetic bulk metallic glasses (BMGs) by an injection casting with applying external magnetic field during solidification has been developed. The effect of applying magnetic fields (Ha) on soft magnetic properties and microstructure of Fe75P8.7B5C7Si4.3 alloy sheets with dimension of t × 4 mm × 15 mm (t = 1-3 mm) have been studied. The microstructure shows that the fully amorphous is attained by the applying magnetic field even at the core region of the thicker sheets with t = 3 mm. All studied sheets exhibit similar saturation magnetization of 1.23-1.25 T, but the coercivity and resistivity are strongly dependent on the thickness t and the strength of magnetic field Ha, where Hc increases with t, the slope of Hc versus t decreases with the increase of Ha, and the resistivity increases with the increase of Ha. The changes of coercivity and resistivity are strongly related to the fully amorphous induced by Ha. The external magnetic field has significant improvement in glass forming ability, and thus coercivity and resistivity for the studied sheet, especially for larger t = 3 mm. This study suggests that applying a magnetic field during the casting process provides a useful way to produce high-performance magnetically soft Fe-based BMGs.

  1. 2-(3-Benzoylthioureido)-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid ameliorates metabolic disorders in high-fat diet-fed mice

    PubMed Central

    Zhang, Jin; Zhang, Li-na; Chen, Dong-mei; Fu, Yan-yun; Zhang, Feng; Yang, Ling-ling; Xia, Chun-mei; Jiang, Hao-wen; Tang, Chun-lan; Xie, Zhi-fu; Yang, Fan; Li, Jia; Tang, Jie; Li, Jing-ya

    2015-01-01

    Aim: Sterol-regulatory element binding proteins (SREBPs) are major transcription factors that regulate liver lipid biosynthesis. In this article we reported a novel synthetic compound 2-(3-benzoylthioureido)-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carboxylic acid (ZJ001) that inhibited the SREBP-1c pathway, and effectively reduced hepatic lipid accumulation in diet-induced obesity (DIO) mice. Methods: A luciferase reporter driven by an SRE-containing promoter transfected into HepG2 cells was used to discover the compound. Two approaches were used to evaluate the lipid-lowering effects of ZJ001: (1) diet-induced obesity (DIO) mice that were treated with ZJ001 (15 mg·kg−1·d−1, po) for 7 weeks; and (2) HepG2 cells and primary hepatocytes used as in vitro models. Results: ZJ001 (10, 20 μmol/L) dose-dependently inhibited the activity of SRE-containing promoter. ZJ001 administration ameliorated lipid metabolism and improved glucose tolerance in DIO mice, accompanied by significantly reduced mRNA levels of SREBP-1C and SREBP-2, and their downstream genes. In HepG2 cells and insulin-treated hepatocytes, ZJ001 (10–40 μmol/L) dose-dependently inhibited lipid synthesis, and reduced mRNA levels of SREBP-1C and SREBP-2, and their downstream genes. Furthermore, ZJ001 dose-dependently increased the phosphorylation of AMPK and regulatory-associated protein of mTOR (Raptor), and suppressed the phosphorylation of mTOR in insulin-treated hepatocytes. Moreover, ZJ001 increased the ADP/ATP ratio in insulin-treated hepatocytes. Conclusion: ZJ001 exerts multiple beneficial effects in diet-induced obesity mice. Its lipid-lowering effects may result from the suppression of mTORC1, which regulates SREBP-1c transcription. The results suggest that the SREBP-1c pathway may be a potential therapeutic target for the treatment of lipid metabolic disorders. PMID:25832429

  2. The attack of titanium-6 wt% aluminium-4 wt% vanadium alloy by a molten uranium-5.7 wt% manganese alloy at 1015 °C

    NASA Astrophysics Data System (ADS)

    Moran, F. J.; Jarman, R. A.

    1991-06-01

    The liquid metal corrosion (LMC) resistance of the alloy Ti-6 wt% Al-4 wt% V (IMI 318) in contact with molten U-5.7 wt% Mn has been assessed. The uranium alloy was melted at 1015 °C under vacuum in hemispherical IMI 318 alloy crucibles. The attack rate of the molten alloy on the IMI 318, for times up to 3 h, was estimated from metallography and by chemical analysis of the resolidified uranium melt. The mechanism of the LMC process was examined with optical and electron microscopy allied with EDAX and microhardness tests. Melt saturation occurred after one hour and titanium-rich (approximately 80 wt% Ti) dendrites began to nucleate and grow in the uranium melt. This result was predicted by the relevant equilibrium phase diagrams. During the LMC reaction, an interface (diffusion) layer grew in IMI 318 alloy where it contacted the uranium alloy melt. The levels of Ti and U changed with test time and distance across this interface, with the Ti level falling at the melt/IMI 318 surface and the U increasing at the same point. The mean LMC rate was initially rapid, 1.45 mm/h after 15 min but fell to 0.3 mm/h at 3 h. The conclusions were that the LMC reaction was diffusion-controlled, with the slow self-diffusion of β-titanium most likely to be the rate determining step. The reaction probably follows parabolic rate-kinetics as do other diffusion-controlled processes. The attack front was generally uniform with no clear evidence of preferential attack.

  3. Exploring the binding mechanism of 5-hydroxy-3‧,4‧,7-trimethoxyflavone with bovine serum albumin: Spectroscopic and computational approach

    NASA Astrophysics Data System (ADS)

    Sudha, A.; Srinivasan, P.; Thamilarasan, V.; Sengottuvelan, N.

    2016-03-01

    The current study was carried out to investigate the binding mechanism of a potential flavonoid compound 5-hydroxy-3‧,4‧,7-trimethoxyflavone (HTMF) with bovine serum albumin (BSA) using ultraviolet-visible, fluorescence, circular dichroism (CD) spectral measurements along with molecular docking and molecular dynamics (MD) simulation. It was confirmed from fluorescence spectra that the intrinsic fluorescence of BSA was robustly quenched by HTMF through a static quenching mechanism. The number of binding sites (n) for HTMF binding on BSA was found to be about one. The thermodynamic parameters estimated from the van't Hoff plot specified that hydrophobic force was the predominant force in the HTMF-BSA complex and there also exist hydrogen bonds and electrostatic interactions. The effect of HTMF on the BSA conformation examined using CD studies revealed that there is a decrease in the helical content of BSA upon HTMF interaction. The results of molecular docking study shed light on the binding mode which exposed that HTMF bind within the hydrophobic pocket of the subdomain IIIA of BSA. The stability of HTMF-BSA complex with respect to free protein was analyzed from the molecular dynamic studies. The electronic structure analysis of HTMF was achieved by using density functional theory (DFT) calculations at B3LYP/6-31G** level to support its antioxidant role. The results of computational analysis are in good consistence with the experimental data and the present findings suggested that HTMF exhibits a good binding propensity to BSA protein which will be helpful for the drug design.

  4. Crystal structure of 4,4′-[(1,3,5,7-tetra­oxo-1,3,3a,4,4a,5,7,7a,8,8a-deca­hydro-4,8-etheno­pyrrolo­[3,4-f]iso­indole-2,6-di­yl)bis­(methyl­ene)]bis­(pyridin-1-ium) dinitrate

    PubMed Central

    Liu, Zhimin

    2015-01-01

    In the title salt, C24H22N4O4 2+·2NO3 −, the cation is U-shaped with the two iso­indole dione rings inclined to one another by 60.41 (13)°, while the two outer pyridine rings are inclined to one another by 2.77 (12)°. The dihedral angles between the pyridine ring and the adjacent iso­indole dione ring are 71.82 (12) and 86.44 (13)°. In the crystal, each nitrate anion is linked to a protonated pyridine ring by N—H⋯O hydrogen bonds. These units are linked by a series of C—H⋯O hydrogen bonds, forming a three-dimensional structure. PMID:26870565

  5. Synthesis and pharmacological properties of 1-[2-hydroxy-3-(4-o,m,p-halogenophenyl)- and 3-(4-m-chlorophenyl)-1-piperazinyl]propyl derivatives of amides of 7-methyl-3-phenyl-2,4-dioxo-1,2,3,4-tetrahydropyrido [2,3-d]pyrimidine-5-carboxylic acid with analgesic and sedative activities.

    PubMed

    Sabiniarz, Aleksandra; Sladowska, Helena; Filipek, Barbara; Sapa, Jacek; Dudek, Magdalena; Slepokura, Katarzyna

    2007-01-01

    Synthesis of 1-[2-hydroxy-3-(4-o,m,p-halogenophenyl)- and 3-(4-m-chlorophenyl)-1-piperazinyl]propyl derivatives of amides of 7-methyl-3-phenyl-2,4-dioxo-1,2,3,4-tetrahydropyrido[2,3-d]pyrimidine-5-carboxylic acid (18, 20-23, 25, 27-30 and 19, 24, 26) is described. All substances were active as analgesic agents in "writhing syndrome" test and except of 18 and 23 they acted stronger than acetylsalicylic acid. All final derivatives tested significantly suppressed the spontaneous locomotor activity of mice. PMID:18536164

  6. Beyond Chapter 4.7.

    PubMed

    Bandler, Lilon Gretl

    2015-12-01

    Chapter 4.7 of the National Statement on Ethical Conduct in Human Research refers specifically to Aboriginal and Torres Strait Islander Peoples. It lays out the points at which researchers working with Aboriginal and Torres Strait Islanders must consider their approach, and the engagement with individuals, communities or groups who are involved in or affected by their research. History, of Australia and of research involving Aboriginal and Torres Strait Islander Australians, has informed this approach. The response to that history has been a rational, institutionalised, systematic demand for a different perception of what should direct research and research processes to ensure engagement with and service to the community with whom the researchers wish to do the work. This paper considers whether these principles could inform the approach to other research work. PMID:27135116

  7. Investigations on the synthesis and pharmacological properties of amides of 7-methyl-3-phenyl-1-[2-hydroxy-3-(4-phenyl-1-piperazinyl)propyl]-2,4- dioxo-1,2,3,4-tetrahydropyrido[2,3-d]-pyrimidine-5-carboxylic acid.

    PubMed

    Sladowska, H; Sieklucka-Dziuba, M; Rajtar, G; Sadowski, M; Kleinrok, Z

    1999-01-01

    Synthesis of amides of 7-methyl-3-phenyl-2,4-dioxo-1,2,3,4- tetrahydropyrido[2,3-d]pyrimidine-5-carboxylic acid (6-10) and their 1-[2-hydroxy-3(4-phenyl-1-piperazinyl)propyl] derivatives (11-15) are described. Some of them displayed strong analgesic activity. PMID:10668178

  8. 4 CFR 7.7 - Other appeals and grievances.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 4 Accounts 1 2010-01-01 2010-01-01 false Other appeals and grievances. 7.7 Section 7.7 Accounts GOVERNMENT ACCOUNTABILITY OFFICE PERSONNEL SYSTEM PERSONNEL RELATIONS AND SERVICES § 7.7 Other appeals and grievances. The personnel system shall provide procedures for the processing of complaints and...

  9. New open-framework in the uranyl vanadates A{sub 3}(UO{sub 2}){sub 7}(VO{sub 4}){sub 5}O (A=Li, Ag) with intergrowth structure between A(UO{sub 2}){sub 4}(VO{sub 4}){sub 3} and A{sub 2}(UO{sub 2}){sub 3}(VO{sub 4}){sub 2}O

    SciTech Connect

    Obbade, S. Renard, C.; Abraham, F.

    2009-03-15

    New uranyl vanadates A{sub 3}(UO{sub 2}){sub 7}(VO{sub 4}){sub 5}O (M=Li (1), Na (2), Ag (3)) have been synthesized by solid-state reaction and their structures determined from single-crystal X-ray diffraction data for 1 and 3. The tetragonal structure results of an alternation of two types of sheets denoted S for {sub {infinity}}{sup 2}[UO{sub 2}(VO{sub 4}){sub 2}]{sup 4-} and D for {sub {infinity}}{sup 2}[(UO{sub 2}){sub 2}(VO{sub 4}){sub 3}]{sup 5-} built from UO{sub 6} square bipyramids and connected through VO{sub 4} tetrahedra to {sub {infinity}}{sup 1}[U(3)O{sub 5}-U(4)O{sub 5}]{sup 8-} infinite chains of edge-shared U(3)O{sub 7} and U(4)O{sub 7} pentagonal bipyramids alternatively parallel to a- and b-axis to construct a three-dimensional uranyl vanadate arrangement. It is noticeable that similar {sub {infinity}}[UO{sub 5}]{sup 4-} chains are connected only by S-type sheets in A{sub 2}(UO{sub 2}){sub 3}(VO{sub 4}){sub 2}O and by D-type sheets in A(UO{sub 2}){sub 4}(VO{sub 4}){sub 3}, thus A{sub 3}(UO{sub 2}){sub 7}(VO{sub 4}){sub 5}O appears as an intergrowth structure between the two previously reported series. The mobility of the monovalent ion in the mutually perpendicular channels created in the three-dimensional arrangement is correlated to the occupation rate of the sites and by the geometry of the different sites occupied by either Na, Ag or Li. Crystallographic data: 293 K, Bruker X8-APEX2 X-ray diffractometer equipped with a 4 K CCD detector, MoK{alpha}, {lambda}=0.71073 A, tetragonal symmetry, space group P4-bar m2, Z=1, full-matrix least-squares refinement on the basis of F{sup 2}; 1,a=7.2794(9) A, c=14.514(4) A, R1=0.021 and wR2=0.048 for 62 parameters with 782 independent reflections with I{>=}2{sigma}(I); 3, a=7.2373(3) A, c=14.7973(15) A, R1=0.041 and wR2=0.085 for 60 parameters with 1066 independent reflections with I{>=}2{sigma}(I). - Abstract: A view of the three-dimensional structure of Li{sub 3}(UO{sub 2}){sub 7}(VO{sub 4}){sub 5}O

  10. 5 CFR 1631.7 - Initial determination.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Initial determination. 1631.7 Section 1631.7 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD AVAILABILITY OF RECORDS Production or Disclosure of Records Under the Freedom of Information Act, 5 U.S.C. 552 § 1631.7...

  11. Effect of fullerene intercalation on the conformation and packing of poly-(2-methoxy-5-(3'-7'-dimethyloctyloxy)-1,4-phenylenevinylene).

    PubMed

    Wise, Adam J; Precit, Mimi R; Papp, Alexandra M; Grey, John K

    2011-08-01

    Photoluminescence (PL) and resonance Raman spectroscopy are used to track changes in the conformations and packing of poly-(2-methoxy-5-(3'-7'-dimethyloctyloxy)-1,4-phenylenevinylene) (MDMO-PPV) chains with the addition of [6,6]-phenyl-C(61)-butyric acid methyl ester (PCBM) molecules. PL lineshapes of MDMO-PPV thin films as a function of annealing time were first measured to determine the spectroscopic signatures of chain conformations and packing in the absence of PCBM. Annealing results in enhanced interchain interactions leading to red-shifts of PL 0-0 transitions by up to ∼300 cm(-1) and apparent increases of the line shape Huang-Rhys factors. Wavelength-dependent PL lifetimes of as-cast and films annealed for short times (∼30 s) are nonexponential with an instrument-limited component of ∼100 ps and a ∼350 ps component. With longer annealing times, decays become single exponential with an average lifetime of ∼1 ns indicating that all excitations efficiently funnel to strongly coupled interchain sites. Addition of PCBM disrupts MDMO-PPV interchain interactions causing PL 0-0 transitions to blue-shift, increases in line width, and decreases in apparent Huang-Rhys factors. Resonance Raman spectra of MDMO-PPV/PCBM thin films with variable PCBM weight fractions (∼50:1 up to 1:8 w/w) were then measured using short (488 nm) and long (568 nm) excitation wavelengths. The out-of-plane vinylene C-H wag mode of MDMO-PPV (∼964 cm(-1)) showed pronounced increases in intensity of up to ∼30% and red-shifts of up to 5 cm(-1) with increasing PCBM content. These changes result from a decrease of planarity between chain segments that suppresses interchain interactions. Furthermore, red-shifts of up to ∼4 cm(-1) were observed for the C═C symmetric stretch of the MDMO-PPV vinylene group (∼1625 cm(-1)) with 488 nm excitation. The sensitivity of the MDMO-PPV vinylene group vibrations with PCBM indicates preferential interactions between these two molecules and is

  12. Pump-probe photoelectron velocity-map imaging of autoionizing singly excited 4s{sup 1}4p{sup 6}np{sup 1}(n=7,8) and doubly excited 4s{sup 2}4p{sup 4}5s{sup 1}6p{sup 1} resonances in atomic krypton

    SciTech Connect

    Doughty, Benjamin; Haber, Louis H.; Leone, Stephen R.

    2011-10-15

    Pump-probe photoelectron velocity-map imaging, using 27-eV high-harmonic excitation and 786-nm ionization, is used to resolve overlapping autoionizing resonances in atomic krypton, obtaining two-photon photoelectron angular distributions (PADs) for singly and doubly excited states. Two features in the photoelectron spectrum are assigned to singly excited 4s{sup 1}4p{sup 6}np{sup 1} (n = 7,8) configurations and four features provide information about double excitation configurations. The anisotropy parameters for the singly excited 7p configuration are measured to be {beta}{sub 2} = 1.61 {+-} 0.06 and {beta}{sub 4} = 1.54 {+-} 0.16 while the 8p configuration gives {beta}{sub 2} = 1.23 {+-} 0.19 and {beta}{sub 4} = 0.60 {+-} 0.15. These anisotropies most likely represent the sum of overlapping PADs from states of singlet and triplet spin multiplicities. Of the four bands corresponding to ionization of doubly excited states, two are assigned to 4s{sup 2}4p{sup 4}5s{sup 1}6p{sup 1} configurations that are probed to different J-split ion states. The two remaining doubly excited states are attributed to a previously observed, but unassigned, resonance in the vacuum-ultraviolet photoabsorption spectrum. The PADs from each of the double excitation states are also influenced by overlap from neighboring states that are not completely spectrally resolved. The anisotropies of the observed double excitation states are reported, anticipating future theoretical and experimental work to separate the overlapping PADs into the state resolved PADs. The results can be used to test theories of excited state ionization.

  13. Crystal chemistry of anhydrous Li uranyl phosphates and arsenates. II. Tubular fragments and cation-cation interactions in the 3D framework structures of Li{sub 6}[(UO{sub 2}){sub 12}(PO{sub 4}){sub 8}(P{sub 4}O{sub 13})], Li{sub 5}[(UO{sub 2}){sub 13}(AsO{sub 4}){sub 9}(As{sub 2}O{sub 7})], Li[(UO{sub 2}){sub 4}(AsO{sub 4}){sub 3}] and Li{sub 3}[(UO{sub 2}){sub 7}(AsO{sub 4}){sub 5}O

    SciTech Connect

    Alekseev, Evgeny V.; Krivovichev, Sergey V.; Depmeier, Wulf

    2009-11-15

    Single crystals of the new compounds Li{sub 6}[(UO{sub 2}){sub 12}(PO{sub 4}){sub 8}(P{sub 4}O{sub 13})] (1), Li{sub 5}[(UO{sub 2}){sub 13}(AsO{sub 4}){sub 9}(As{sub 2}O{sub 7})] (2), Li[(UO{sub 2}){sub 4}(AsO{sub 4}){sub 3}] (3) and Li{sub 3}[(UO{sub 2}){sub 7}(AsO{sub 4}){sub 5}O)] (4) have been prepared using high-temperature solid state reactions. The crystal structures have been solved by direct methods: 1-monoclinic, C2/m, a=26.963(3) A, b=7.063(1) A, c=19.639(1) A, beta=126.890(4){sup o}, V=2991.2(6) A{sup 3}, Z=2, R{sub 1}=0.0357 for 3248 unique reflections with |F{sub 0}|>=4sigma{sub F}; 2-triclinic, P1-bar, a=7.1410(8) A, b=13.959(1) A, c=31.925(1) A, alpha=82.850(2){sup o}, beta=88.691(2){sup o}, gamma=79.774(3){sup o}, V=3107.4(4) A{sup 3}, Z=2, R{sub 1}=0.0722 for 9161 unique reflections with |F{sub 0}|>=4sigma{sub F}; 3-tetragonal, I4{sub 1}/amd, a=7.160(3) A, c=33.775(9) A, V=1732(1) A{sup 3}, Z=4, R{sub 1}=0.0356 for 318 unique reflections with |F{sub 0}|>=4sigma{sub F}; 4-tetragonal, P4-bar, a=7.2160(5) A, c=14.6540(7) A, V=763.04(8) A{sup 3}, Z=1, R{sub 1}=0.0423 for 1600 unique reflections with |F{sub 0}|>=4sigma{sub F}. Structures of all the phases under consideration are based on complex 3D frameworks consisting of different types of uranium polyhedra (UO{sub 6} and UO{sub 7}) and different types of tetrahedral TO{sub 4} anions (T=P or As): PO{sub 4} and P{sub 4}O{sub 13} in 1, AsO{sub 4} and As{sub 2}O{sub 7} in 2, and single AsO{sub 4} tetrahedra in 3 and 4. In the structures of 1 and 2, UO{sub 7} pentagonal bipyramids share edges to form (UO{sub 5}){sub i}nfinity chains extended along the b axis in 1 and along the a axis in 2. The chains are linked via single TO{sub 4} tetrahedra into tubular units with external diameters of 11 A in 1 and 11.5 A in 2, and internal diameters of 4.1 A in 1 and 4.5 A in 2. The channels accommodate Li{sup +} cations. The tubular units are linked into 3D frameworks by intertubular complexes. Structures of 3 and 4

  14. z ≳ 7 Galaxies with Red Spitzer/IRAC [3.6]-[4.5] Colors in the Full CANDELS Data Set: The Brightest-Known Galaxies at z ~ 7-9 and a Probable Spectroscopic Confirmation at z = 7.48

    NASA Astrophysics Data System (ADS)

    Roberts-Borsani, G. W.; Bouwens, R. J.; Oesch, P. A.; Labbe, I.; Smit, R.; Illingworth, G. D.; van Dokkum, P.; Holden, B.; Gonzalez, V.; Stefanon, M.; Holwerda, B.; Wilkins, S.

    2016-06-01

    We identify four unusually bright (H {}160,{AB} < 25.5) galaxies from Hubble Space Telescope (HST) and Spitzer CANDELS data with probable redshifts z ∼ 7–9. These identifications include the brightest-known galaxies to date at z ≳ 7.5. As Y-band observations are not available over the full CANDELS program to perform a standard Lyman-break selection of z > 7 galaxies, we employ an alternate strategy using deep Spitzer/IRAC data. We identify z ∼ 7.1–9.1 galaxies by selecting z ≳ 6 galaxies from the HST CANDELS data that show quite red IRAC [3.6]‑[4.5] colors, indicating strong [O iii]+Hβ lines in the 4.5 μm band. This selection strategy was validated using a modest sample for which we have deep Y-band coverage, and subsequently used to select the brightest z ≥ 7 sources. Applying the IRAC criteria to all HST-selected optical dropout galaxies over the full ∼900 arcmin2 of the CANDELS survey revealed four unusually bright z ∼ 7.1, 7.6, 7.9, and 8.6 candidates. The median [3.6]‑[4.5] color of our selected z ∼ 7.1–9.1 sample is consistent with rest-frame [O iii]+Hβ EWs of ∼1500 Å in the [4.5] band. Keck/MOSFIRE spectroscopy has been independently reported for two of our selected sources, showing Lyα at redshifts of 7.7302 ± 0.0006 and {8.683}-0.004+0.001, respectively. We present similar Keck/MOSFIRE spectroscopy for a third selected galaxy with a probable 4.7σ Lyα line at z spec = 7.4770 ± 0.0008. All three have H160-band magnitudes of ∼25 mag and are ∼0.5 mag more luminous (M 1600 ∼ ‑22.0) than any previously discovered z ∼ 8 galaxy, with important implications for the UV luminosity function (LF). Our three brightest and highest redshift z > 7 galaxies all lie within the CANDELS-EGS field, providing a dramatic illustration of the potential impact of field-to-field variance.

  15. 7 CFR 3550.5 - Environmental requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... accordance with the requirements provided in 7 CFR part 1940, subpart G which addresses environmental... 7 Agriculture 15 2014-01-01 2014-01-01 false Environmental requirements. 3550.5 Section 3550.5... AGRICULTURE DIRECT SINGLE FAMILY HOUSING LOANS AND GRANTS General § 3550.5 Environmental requirements....

  16. 7 CFR 868.5 - Administrator.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 7 2010-01-01 2010-01-01 false Administrator. 868.5 Section 868.5 Agriculture... FOR CERTAIN AGRICULTURAL COMMODITIES Regulations Administration § 868.5 Administrator. The Administrator, under the authority delegated by the Secretary, is charged with administering the programs...

  17. 7 CFR 868.5 - Administrator.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 7 2012-01-01 2012-01-01 false Administrator. 868.5 Section 868.5 Agriculture... FOR CERTAIN AGRICULTURAL COMMODITIES Regulations Administration § 868.5 Administrator. The Administrator, under the authority delegated by the Secretary, is charged with administering the programs...

  18. 7 CFR 868.5 - Administrator.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 7 2014-01-01 2014-01-01 false Administrator. 868.5 Section 868.5 Agriculture... FOR CERTAIN AGRICULTURAL COMMODITIES Regulations Administration § 868.5 Administrator. The Administrator, under the authority delegated by the Secretary, is charged with administering the programs...

  19. 7 CFR 868.5 - Administrator.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 7 2011-01-01 2011-01-01 false Administrator. 868.5 Section 868.5 Agriculture... FOR CERTAIN AGRICULTURAL COMMODITIES Regulations Administration § 868.5 Administrator. The Administrator, under the authority delegated by the Secretary, is charged with administering the programs...

  20. 7 CFR 868.5 - Administrator.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 7 2013-01-01 2013-01-01 false Administrator. 868.5 Section 868.5 Agriculture... FOR CERTAIN AGRICULTURAL COMMODITIES Regulations Administration § 868.5 Administrator. The Administrator, under the authority delegated by the Secretary, is charged with administering the programs...

  1. 7 CFR 772.5 - Security maintenance.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 7 2014-01-01 2014-01-01 false Security maintenance. 772.5 Section 772.5 Agriculture... SPECIAL PROGRAMS SERVICING MINOR PROGRAM LOANS § 772.5 Security maintenance. (a) General. Borrowers are responsible for maintaining the collateral that is serving as security for their Minor Program loan...

  2. Cognitive and neural correlates of the 5-repeat allele of the dopamine D4 receptor gene in a population lacking the 7-repeat allele.

    PubMed

    Takeuchi, Hikaru; Tomita, Hiroaki; Taki, Yasuyuki; Kikuchi, Yoshie; Ono, Chiaki; Yu, Zhiqian; Sekiguchi, Atsushi; Nouchi, Rui; Kotozaki, Yuka; Nakagawa, Seishu; Miyauchi, Carlos Makoto; Iizuka, Kunio; Yokoyama, Ryoichi; Shinada, Takamitsu; Yamamoto, Yuki; Hanawa, Sugiko; Araki, Tsuyoshi; Hashizume, Hiroshi; Kunitoki, Keiko; Sassa, Yuko; Kawashima, Ryuta

    2015-04-15

    The 5-repeat allele of a common length polymorphism in the gene that encodes the dopamine D4 receptor (DRD4) is robustly associated with the risk of attention deficit hyperactivity disorder (ADHD) and substantially exists in Asian populations, which have a lower ADHD prevalence. In this study, we investigated the effect of this allele on microstructural properties of the brain and on its functional activity during externally directed attention-demanding tasks and creative performance in the 765 Asian subjects. For this purpose, we employed diffusion tensor imaging, N-back functional magnetic resonance imaging paradigms, and a test to measure creativity by divergent thinking. The 5-repeat allele was significantly associated with increased originality in the creative performance, increased mean diffusivity (the measure of how the tissue includes water molecules instead of neural and vessel components) in the widespread gray and white matter areas of extensive areas, particularly those where DRD4 is expressed, and reduced task-induced deactivation in the areas that are deactivated during the tasks in the course of both the attention-demanding working memory task and simple sensorimotor task. The observed neural characteristics of 5-repeat allele carriers may lead to an increased risk of ADHD and behavioral deficits. Furthermore, the increased originality of creative thinking observed in the 5-repeat allele carriers may support the notion of the side of adaptivity of the widespread risk allele of psychiatric diseases. PMID:25659462

  3. Crystal structure of (+)-N-[(1R,5S,6S,9S)-5-hydroxy-methyl-3,3,9-trimethyl-8-oxo-2,4,7-trioxabi-cyclo-[4.3.0]nonan-9-yl]acetamide.

    PubMed

    Oishi, Takeshi; Tsuzaki, Shun; Sugai, Tomoya; Sato, Takaaki; Chida, Noritaka

    2016-05-01

    In the title compound, C12H19NO6, the six-membered 1,3-dioxane ring adopts a chair-like conformation. The seat of this chair, containing two O atoms, is essentially planar, with a maximum deviation of 0.0021 (12) Å. The five-membered oxolane ring cis-fused to the 1,3-dioxane ring adopts an envelope form. The bridgehead C atom at the flap, which is bonded to the tetra-substituted C atom of the oxolane ring, deviates from the mean plane of other ring atoms by 0.539 (4) Å. In the crystal, classical O-H⋯O and N-H⋯O hydrogen bonds link the mol-ecules into a sheet structure enclosing an R 4 (4)(24) graph-set motif. Weak inter-molecular C-H⋯O inter-actions support the sheet formation. PMID:27308035

  4. Crystal structure of (2-{3-[4-(4′-cyano­biphenyl-4-yl­oxy)but­oxy]pyridin-2-yl-κN}-5-(dodec­yloxy)phenyl-κC 1)(9-oxo­tetra­cos-7-en-7-olato-κ2 O,O′)platinum(II)

    PubMed Central

    Luo, Kaijun; Liu, Hanlin; Guo, Qing; Luo, Daibing

    2014-01-01

    The central PtII atom in the title compound, [Pt(C40H47N2O3)(C24H45O2)], has a slightly distorted square-planar coordination environment. The dihedral angle between the plane formed by Pt and the chelating C and N atoms and that formed by Pt and the chelating O atoms is 2.1 (3)°. The angle between the planes of the two rings in the biphenyl-4-carbo­nitrile unit is 35.1 (5)°. One lateral alkane chain is disordered over two sets of sites with site occupancy factors in a 0.595 (7):0.405 (7) ratio. PMID:25553005

  5. Theoretical studies on vicinal-tetrazine compounds: furoxano-1,2,3,4-tetrazine-1,3,5-trioxide (FTTO-α) and furoxano-1,2,3,4-tetrazine-1,3,7-trioxide (FTTO-β).

    PubMed

    Wang, Tianyi; Zhang, Tao; Xu, Liwen; Wu, Xionghui; Gong, Xuedong; Xia, Mingzhu

    2014-12-01

    The derivatives of 1,2,3,4-tetrazine may be promising candidates of high-energy density compounds and are receiving more and more attention. In this study, two 1,2,3,4-tetrazines, furoxano-1,2,3,4-tetrazine-1,3,5-trioxide (FTTO-α) and furoxano-1,2,3,4-tetrazine-1,3,7-trioxide (FTTO-β), were theoretically studied. The geometrical structures in gas phase were studied at the B3LYP/6-311++G(d,p) level of density functional theory (DFT). The gas phase enthalpies of formation were calculated by the homodesmotic reaction method. The enthalpies of sublimation and solid phase enthalpies of formation were predicted with corrections of electrostatic potential method at the B3PW91/6-31G(d,p) level. The detonation properties were estimated with the Kamlet-Jacobs equations based on the predicted densities and enthalpies of formation in solid state. The available free space in the lattice was calculated to evaluate their stability. Calculations of potential energy surface and structure interconversion thermodynamics under different temperatures were carried out to further confirm their stability. FTTOs have better performance than HMX and FTDO but are easy to decompose to 5,6-dinitroso-v-tetrazine 1,3-dioxide. A synthesis route for FTTO-β was proposed to provide a consideration for the further study. We believe FTTOs could be the key compounds to synthesize other v-tetrazines such as TTTO. PMID:25413679

  6. 48 CFR 915.404-4-70-7 - Alternative techniques.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Alternative techniques. 915.404-4-70-7 Section 915.404-4-70-7 Federal Acquisition Regulations System DEPARTMENT OF ENERGY CONTRACTING METHODS AND CONTRACT TYPES CONTRACTING BY NEGOTIATION Contract Pricing 915.404-4-70-7...

  7. The genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4

    PubMed Central

    Janto, Benjamin; Ahmed, Azad; Ito, Masahiro; Liu, Jun; Hicks, David B.; Pagni, Sarah; Fackelmayer, Oliver J.; Smith, Terry-Ann; Earl, Joshua; Elbourne, Liam D.H.; Hassan, Karl; Paulsen, Ian T.; Kolstø, Anne-Brit; Tourasse, Nicolas J.; Ehrlich, Garth D.; Boissy, Robert; Ivey, D. Mack; Li, Gang; Xue, Yanfen; Ma, Yanhe; Hu, Fen Z.; Krulwich, Terry A.

    2011-01-01

    Summary Bacillus pseudofirmus OF4 is an extreme but facultative alkaliphile that grows non-fermentatively in a pH range from 7.5 to above 11.4 and can withstand large sudden increases in external pH. It is a model organism for studies of bioenergetics at high pH, at which energy demands are higher than at neutral pH because both cytoplasmic pH homeostasis and ATP synthesis require more energy. The alkaliphile also tolerates a cytoplasmic pH > 9.0 at external pH values at which the pH homeostasis capacity is exceeded, and manages other stresses that are exacerbated at alkaline pH, e.g. sodium, oxidative and cell wall stresses. The genome of B. pseudofirmus OF4 includes two plasmids that are lost from some mutants without viability loss. The plasmids may provide a reservoir of mobile elements that promote adaptive chromosomal rearrangements under particular environmental conditions. The genome also reveals a more acidic pI profile for proteins exposed on the outer surface than found in neutralophiles. A large array of transporters and regulatory genes are predicted to protect the alkaliphile from its overlapping stresses. In addition, unanticipated metabolic versatility was observed, which could ensure requisite energy for alkaliphily under diverse conditions. PMID:21951522

  8. Effects of 3,3',4,4',5-pentachlorobiphenyl (PCB 126) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) injected into the yolks of chicken (Gallus domesticus) eggs prior to incubation.

    PubMed

    Powell, D C; Aulerich, R J; Meadows, J C; Tillitt, D E; Giesy, J P; Stromberg, K L; Bursian, S J

    1996-10-01

    The yolks of White Leghorn chicken (Gallus domesticus) eggs were injected prior to incubation with either 3,3',4,4',5-pentachlorobiphenyl (PCB 126) at doses ranging from 0.1 to 12.8 microg/kg egg or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at doses ranging from 0.04 to 0.64 microg/kg egg. Chicks were subjected to necropsy within 24 h of hatching. The brain, bursa, heart, liver, and spleen were removed and weighed. Assessment of the rate of hatching indicated an LD50+/-S.E. of 2.3+/-0.19 microg/kg egg (7. 1+/-0.58 nmol/kg egg) for PCB 126 and 0.15 +/- 0.012 microg/kg egg (0.47 +/- 0.037 nmol/kg egg) for TCDD. No significant differences in the incidence of developmental abnormalities (structural defects and edema) were observed in TCDD-exposed embryos, while PCB 126 caused significantly more developmental abnormalities at 3.2, 6.4, and 12.8 microg/kg egg than the vehicle control. PCB 126 caused lower hatchling weights and greater relative brain, heart, and liver weights when compared to the vehicle control group at a dose of 3.2 microg/kg egg which is greater than the LD50. TCDD at 0.08 microg/kg egg caused relative bursa weights to be less than those of the vehicle control. A toxic equivalency factor (TEF) of 0.07 was determined for PCB 126 in relation to TCDD based on overt lethality. PMID:8854835

  9. Ring-strain release in neutral and dicationic 7,8,17,18-tetra-bromo-5,10,15,20-tetra-phenyl-porphyrin: crystal structures of C44H26Br4N4 and C44H28Br4N4 (2+)·2ClO4 (-)·3CH2Cl2.

    PubMed

    Scheidt, W Robert; Duval, Hugues F; Oliver, Allen G

    2016-06-01

    Two porphyrin complexes were studied to determine the effects of protonation on ring deformation within the porphyrin. The porphyrin 7,8,17,18-tetra-bromo-5,10,15,20-tetra-phenyl-porphyrin, C44H26Br4N4, was selected because the neutral species is readily doubly protonated to yield a dication, which was crystallized here with perchlorate counter-ions as a di-chloro-methane tris-olvate, C44H28Br4N4 (2+)·2ClO4 (-)·3CH2Cl2. The centrosymmetric neutral species is observed to have a mild 'ruffling' of the pyrrole rings and is essentially planar throughout; intra-molecular N-H⋯N hydrogen bonds occur. In contrast, the dication exhibits considerable deformation, with the pyrrole rings oriented well out of the plane of the porphyrin, resulting in a 'saddle' conformation of the ring. The charged species forms N-H⋯O hydrogen bonds to the perchlorate anions, which lie above and below the plane of the porphyrin ring. Distortions to the planarity of the pyrrole rings in both cases are very minor. The characterization of the neutral species represents a low-temperature redetermination of the previous room-temperature analyses [Zou et al. (1995 ▸). Acta Cryst. C51, 760-761; Rayati et al. (2008 ▸). Polyhedron, pp. 2285-2290], which showed disorder and physically unrealistic displacement parameters. PMID:27308051

  10. Spin dynamics, short-range order, and spin freezing in Y{sub 0.5}Ca{sub 0.5}BaCo{sub 4}O{sub 7}

    SciTech Connect

    Stewart, J. R.; Ehlers, G.; Mutka, H.; Fouquet, P.; Payen, C.; Lortz, R.

    2011-01-15

    Y{sub 0.5}Ca{sub 0.5}BaCo{sub 4}O{sub 7} was recently introduced as a possible candidate for capturing some of the predicted classical spin kagome ground-state features. Stimulated by this conjecture, we have taken up a more complete study of the spin correlations in this compound with neutron scattering methods on a powder sample characterized with high-resolution neutron diffraction and the temperature dependence of magnetic susceptibility and specific heat. We have found that the frustrated near-neighbor magnetic correlations involve not only the kagome planes but concern the full Co sublattice, as evidenced by the analysis of the wave-vector dependence of the short-range order. We conclude from our results that the magnetic moments are located on the Co sublattice as a whole and that correlations extend beyond the two-dimensional kagome planes. We identify intriguing dynamical properties, observing high-frequency fluctuations with a Lorentzian linewidth {Gamma}{<=}20 meV at ambient temperature. On cooling a low-frequency ({approx}1 meV) dynamical component develops alongside the high-frequency fluctuations, which eventually becomes static at temperatures below T{approx_equal}50 K. The high-frequency response with an overall linewidth of {approx}10 meV prevails at T{<=}2 K, coincident with a fully elastic short-range-ordered contribution.

  11. Lithium diffusion in the spinel phase Li4Ti5O12 and in the rocksalt phase Li7Ti5O12 of lithium titanate from first principles

    NASA Astrophysics Data System (ADS)

    Ziebarth, Benedikt; Klinsmann, Markus; Eckl, Thomas; Elsässer, Christian

    2014-05-01

    Lithium titanate (LTO) is a promising candidate as an anode material in future generations of lithium ion batteries due to its high intrinsic safety and stability. In this work, we investigate the diffusion barriers for lithium ions in two different crystal structures of LTO using the density functional theory. Our calculations show that the activation barriers vary between 0.30-0.48 eV for the spinel phase Li4Ti5O12 and between 0.20-0.51 eV in the lithiated rocksalt phase Li7Ti5O12. The origins of the rather broad ranges of activation energies are related to different chemical environments of the diffusion channels due to mixed occupancies of some sites in LTO. Our results reveal that the determination of lithium diffusion constants in LTO can not be carried out by using a single activation barrier. Instead, the local environment of the diffusion paths must be considered to correctly capture the variety of activation barriers. Moreover, we find the sites which have mixed occupation in LTO to trap lithium vacancies in the spinel phase. This effect is not observed in the rocksalt phase. This behavior explains the low lithium diffusivity found in experiments for lithium concentrations in the vicinity of the spinel phase.

  12. 5,5,7,12,14,14-Hexamethyl-1,8-diaza-4,11-diazo­niacyclo­tetra­deca-4,11-diene dichloride trihydrate

    PubMed Central

    Ismail, Wafiuddin; Yamin, Bohari M.; Daran, Jean-Claude

    2012-01-01

    In the title compound, C16H34N4 2+·2Cl−·3H2O, the two protonated N atoms in the macrocyclic ring of the dication are located at diagonally opposite positions. There are two intramolecular N—H⋯N hydrogen bonds in the cation. The crystal structure features O—H⋯Cl, O—H⋯O, C—H⋯Cl and N—H⋯Cl hydrogen bonds. PMID:22590349

  13. 6,7,8,9,10,11,12,13-Octa­hydro-5H-1,3-dithiole[4,5-b][1,4]dithia­cyclo­tridecine-2-thione

    PubMed Central

    Heshmatpour, Felora; Darviche, Fatemeh; Emdadi, Zeynab; Neumueller, Bernhard

    2008-01-01

    In the crystal structure of the title compound, C12H18S5, no significant inter­molecular π–π inter­actions are found. Weak inter­molecular C—S⋯π [S⋯centroid = 3.787 (1) Å] inter­actions and van der Waals forces may be effective in the stabilization of the structure. PMID:21201430

  14. Bis[3α,7α,12α-tris­(4-nitro­benzo­yloxy)-5β-cholan-24-yl] disulfide–ethyl acetate–n-hexane (4/4/1)

    PubMed Central

    Brzezinski, Krzysztof; Tomkiel, Aneta M.; Łotowski, Zenon; Morzycki, Jacek; Dauter, Zbigniew

    2011-01-01

    The crystal structure of the title compound, C90H100N6O24S2·C4H8O2·0.25C6H14, solved and refined against synchrotron diffraction data, contains two formula units in the asymmetric unit with the all-trans n-hexane mol­ecule having half-occupancy and one of the ethyl acetate mol­ecules disordered over two positions. The two symmetry-independent disulfide mol­ecules are assembled by approximate face-to-face and face-to-edge inter­actions between their 4-nitro­benzo­yloxy groups into an inter­twined dimer having a double-helix-type structure. The centrally placed disulfide bridges in the two symmetry-independent mol­ecules exhibit different helicity as shown by the C—S—S—C torsion angles of 71.0 (1) and −92.5 (1)°. PMID:21522786

  15. Long-Term Corrosion Tests of Prototypical SAM2X5 (Fe49.7Cr17.7Mn1.9Mo7.4W1.6B15.2C3.8Si2.4) Coatings

    SciTech Connect

    Farmer, J C; Choi, J S; Saw, C K; Rebak, R H; Day, S D; Lian, T; Hailey, P D; Payer, J H; Branagan, D J; Aprigliano, L F

    2007-05-10

    An iron-based amorphous metal with good corrosion resistance and a high absorption cross-section for thermal neutrons has been developed and is reported here. This amorphous alloy has the approximate formula Fe{sub 49.7}Cr{sub 17.7}Mn{sub 1.9}Mo{sub 7.4}W{sub 1.6}B{sub 15.2}C{sub 3.8}Si{sub 2.4} and is known as SAM2X5. Chromium (Cr), molybdenum (Mo) and tungsten (W) were added to provide corrosion resistance, while boron (B) was added to promote glass formation and the absorption of thermal neutrons. Since this amorphous metal has a higher boron content than conventional borated stainless steels, it provides the nuclear engineer with design advantages for criticality control structures with enhanced safety. While melt-spun ribbons with limited practical applications were initially produced, large quantities (several tons) of gas atomized powder have now been produced on an industrial scale, and applied as thermal-spray coatings on prototypical half-scale spent nuclear fuel containers and neutron-absorbing baskets. These prototypes and other SAM2X5 samples have undergone a variety of corrosion testing, including both salt-fog and long-term immersion testing. The modes and rates of corrosion have been determined in the various environments, and are reported here. While these coatings have less corrosion resistance than melt-spun ribbons and optimized coatings produced in the laboratory, substantial corrosion resistance has been achieved.

  16. 7-Chloro-5-(furan-3-yl)-3-methyl-4H-benzo[e][1,2,4]thiadiazine 1,1-Dioxide as Positive Allosteric Modulator of α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor. The End of the Unsaturated-Inactive Paradigm?

    PubMed

    Citti, Cinzia; Battisti, Umberto M; Cannazza, Giuseppe; Jozwiak, Krzysztof; Stasiak, Natalia; Puja, Giulia; Ravazzini, Federica; Ciccarella, Giuseppe; Braghiroli, Daniela; Parenti, Carlo; Troisi, Luigino; Zoli, Michele

    2016-02-17

    5-Arylbenzothiadiazine type compounds acting as positive allosteric modulators of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPA-PAMs) have received particular attention in the past decade for their nootropic activity and lack of the excitotoxic side effects of direct agonists. Recently, our research group has published the synthesis and biological activity of 7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide (1), one of the most active benzothiadiazine-derived AMPA-PAMs in vitro to date. However, 1 exists as two stereolabile enantiomers, which rapidly racemize in physiological conditions, and only one isomer is responsible for the pharmacological activity. In the present work, experiments carried out with rat liver microsomes show that 1 is converted by hepatic cytochrome P450 to the corresponding unsaturated derivative 2 and to the corresponding pharmacologically inactive benzenesulfonamide 3. Surprisingly, patch-clamp experiments reveal that 2 displays an activity comparable to that of the parent compound. Molecular modeling studies were performed to rationalize these results. Furthermore, mice cerebral microdialysis studies suggest that 2 is able to cross the blood-brain barrier and increases acetylcholine and serotonin levels in the hippocampus. The experimental data disclose that the achiral hepatic metabolite 2 possesses the same pharmacological activity of its parent compound 1 but with an enhanced chemical and stereochemical stability, as well as an improved pharmacokinetic profile compared with 1. PMID:26580317

  17. Discovery of Methyl 4′-Methyl-5-(7-nitrobenzo[c][1,2,5]oxadiazol-4-yl)-[1,1′-biphenyl]-3-carboxylate, an Improved Small-Molecule Inhibitor of c-Myc–Max Dimerization

    PubMed Central

    Yap, Jeremy L.; Sabato, Philip E.; Hu, Angela; Prochownik, Edward V.; Fletcher, Steven

    2014-01-01

    c-Myc is a bHLH-ZIP transcription factor that is responsible for the transcription of a wide range of target genes involved in many cancer-related cellular processes, such as proliferation, differentiation, apoptosis and metabolism. Over-expression of c-Myc has been observed in, and directly contributes to, a variety of human cancers including those of the hematopoietic system, lung, prostate and colon. To become transcriptionally active, c-Myc must first dimerize with Max via its own bHLH-ZIP domain. A proven strategy towards the inhibition of c-Myc oncogenic activity is to interfere with the structural integrity of the c-Myc–Max heterodimer. The small-molecule 10074-G5 is an inhibitor of c-Myc–Max dimerization (IC50 = 146 μM) that operates by binding and stabilizing c-Myc in its monomeric form. Herein, we report on our on-going efforts to optimize the c-Myc–Max inhibitory activity of 10074-G5-related molecules in vitro and in cancer cells that over-express c-Myc. Specifically, we have identified a congener of 10074-G5, termed 3jc48-3, that is about five times as potent (IC50 = 34 μM) at inhibiting c-Myc–Max dimerization as the parent compound. In addition, 3jc48-3 exhibited an approximate two-fold selectivity for c-Myc–Max heterodimers over Max–Max dimers, suggesting that, like its predecessor, its mode of action is through binding c-Myc. 3jc48-3 inhibited the proliferation of c-Myc-over-expressing HL60 and Daudi cells with single-digit micromolar IC50 values by causing growth arrest at the G0/G1 phase. Furthermore, co-immunoprecipitation studies indicated that 3jc48-3 inhibits c-Myc–Max dimerization in cells, which was further substantiated by the specific silencing of a c-Myc-driven luciferase reporter gene. Finally, unlike previously described 10074-G5 analogues, which are rapidly released and/or metabolized by cells following their uptake, 3jc48-3’s intracellular half-life was >17 h. Collectively, these data demonstrate 3jc48-3 to be one of

  18. Na7Cr4(P2O7)4PO4

    PubMed Central

    Bourguiba Fakhar, Noura; Zid, Mohamed Faouzi; Driss, Ahmed

    2013-01-01

    The title compound, hepta­sodium tetra­chromium(III) tetra­kis­(diphosphate) orthophosphate, was synthesized by solid-state reaction. Its structure is isotypic with that of Na7 M 4(P2O7)4PO4 (M = In, Al) compounds and is made up from a three-dimensional [(CrP2O7)4PO4]7− framework with channels running along [001]. The three Na+ cations are located in the voids of the framework. One of the cations is situated on a general position, one is equally disordered around a twofold rotation axis and one is on a fourfold rotoinversion axis. The isolated PO4 tetra­hedron of the anionic framework is also situated on the -4 axis. Structural relationships between the title compound and different diphosphates containing MP2O11 units (M = Mo, V) are discussed. PMID:23723751

  19. 5 CFR 1820.7 - Fees.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Fees. 1820.7 Section 1820.7 Administrative Personnel OFFICE OF SPECIAL COUNSEL FREEDOM OF INFORMATION ACT REQUESTS; PRODUCTION OF RECORDS OR TESTIMONY § 1820.7 Fees. (a) In general. OSC shall charge for processing requests under the FOIA in accordance with paragraph (c) of this...

  20. 5 CFR 1630.7 - Identification requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Identification requirements. 1630.7 Section 1630.7 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD PRIVACY ACT REGULATIONS § 1630.7 Identification requirements. (a) In person. An individual should be prepared to identify himself or herself by signature, i.e., to note...

  1. 5 CFR 1655.7 - Interest rate.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... interest rate established by the Department of the Treasury in effect on the date the TSP record keeper... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Interest rate. 1655.7 Section 1655.7 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD LOAN PROGRAM § 1655.7 Interest rate....

  2. 5 CFR 1655.7 - Interest rate.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... interest rate established by the Department of the Treasury in effect on the date the TSP record keeper... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Interest rate. 1655.7 Section 1655.7 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD LOAN PROGRAM § 1655.7 Interest rate....

  3. 5 CFR 9301.7 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Definitions. 9301.7 Section 9301.7 Administrative Personnel SPECIAL INSPECTOR GENERAL FOR AFGHANISTAN RECONSTRUCTION DISCLOSURE OF RECORDS AND INFORMATION Freedom of Information Act Costs § 9301.7 Definitions. For purposes of this subpart:...

  4. 5 CFR 9301.7 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Definitions. 9301.7 Section 9301.7 Administrative Personnel SPECIAL INSPECTOR GENERAL FOR AFGHANISTAN RECONSTRUCTION DISCLOSURE OF RECORDS AND INFORMATION Freedom of Information Act Costs § 9301.7 Definitions. For purposes of this subpart:...

  5. 5 CFR 7.3 - Citizenship.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Citizenship. 7.3 Section 7.3 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE RULES GENERAL PROVISIONS (RULE VII) § 7.3 Citizenship. (a) No person shall be admitted to competitive examination unless such person is a citizen...

  6. 5 CFR 1820.7 - Fees.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Fees. 1820.7 Section 1820.7 Administrative Personnel OFFICE OF SPECIAL COUNSEL FREEDOM OF INFORMATION ACT REQUESTS; PRODUCTION OF RECORDS OR TESTIMONY § 1820.7 Fees. (a) In general. OSC shall charge for processing requests under the FOIA in accordance with paragraph (c) of this...

  7. 5 CFR 2421.7 - Executive Director.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Executive Director. 2421.7 Section 2421.7... FEDERAL LABOR RELATIONS AUTHORITY MEANING OF TERMS AS USED IN THIS SUBCHAPTER § 2421.7 Executive Director. Executive Director means the Executive Director of the Authority....

  8. 5 CFR 2421.7 - Executive Director.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 3 2010-01-01 2010-01-01 false Executive Director. 2421.7 Section 2421.7... FEDERAL LABOR RELATIONS AUTHORITY MEANING OF TERMS AS USED IN THIS SUBCHAPTER § 2421.7 Executive Director. Executive Director means the Executive Director of the Authority....

  9. 5 CFR 2421.7 - Executive Director.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Executive Director. 2421.7 Section 2421.7... FEDERAL LABOR RELATIONS AUTHORITY MEANING OF TERMS AS USED IN THIS SUBCHAPTER § 2421.7 Executive Director. Executive Director means the Executive Director of the Authority....

  10. 5 CFR 2421.7 - Executive Director.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 3 2011-01-01 2011-01-01 false Executive Director. 2421.7 Section 2421.7... FEDERAL LABOR RELATIONS AUTHORITY MEANING OF TERMS AS USED IN THIS SUBCHAPTER § 2421.7 Executive Director. Executive Director means the Executive Director of the Authority....

  11. 5 CFR 2421.7 - Executive Director.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Executive Director. 2421.7 Section 2421.7... FEDERAL LABOR RELATIONS AUTHORITY MEANING OF TERMS AS USED IN THIS SUBCHAPTER § 2421.7 Executive Director. Executive Director means the Executive Director of the Authority....

  12. 5 CFR 1655.7 - Interest rate.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... processes the paper application or on the date the request is entered on the TSP Web site. (b) The interest... 5 Administrative Personnel 3 2012-01-01 2012-01-01 false Interest rate. 1655.7 Section 1655.7 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD LOAN PROGRAM § 1655.7 Interest rate....

  13. 5 CFR 1655.7 - Interest rate.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... processes the paper application or on the date the request is entered on the TSP Web site. (b) The interest... 5 Administrative Personnel 3 2013-01-01 2013-01-01 false Interest rate. 1655.7 Section 1655.7 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD LOAN PROGRAM § 1655.7 Interest rate....

  14. 5 CFR 1655.7 - Interest rate.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... processes the paper application or on the date the request is entered on the TSP Web site. (b) The interest... 5 Administrative Personnel 3 2014-01-01 2014-01-01 false Interest rate. 1655.7 Section 1655.7 Administrative Personnel FEDERAL RETIREMENT THRIFT INVESTMENT BOARD LOAN PROGRAM § 1655.7 Interest rate....

  15. 5 CFR 7.2 - Reemployment rights.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 1 2014-01-01 2014-01-01 false Reemployment rights. 7.2 Section 7.2 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE RULES GENERAL PROVISIONS (RULE VII) § 7.2 Reemployment rights. OPM, whenever it determines it to be necessary, shall prescribe regulations governing...

  16. 5 CFR 7.3 - Citizenship.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 1 2014-01-01 2014-01-01 false Citizenship. 7.3 Section 7.3 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE RULES GENERAL PROVISIONS (RULE VII) § 7.3 Citizenship. (a) No person shall be admitted to competitive examination unless such person is a citizen...

  17. 7 CFR 3550.5 - Environmental requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... accordance with the requirements provided in 7 CFR part 1940, subpart G which addresses environmental requirements and 7 CFR part 1924, subpart A, which addresses lead-based paint. ... 7 Agriculture 15 2010-01-01 2010-01-01 false Environmental requirements. 3550.5 Section...

  18. 7 CFR 3550.5 - Environmental requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... accordance with the requirements provided in 7 CFR part 1940, subpart G which addresses environmental requirements and 7 CFR part 1924, subpart A, which addresses lead-based paint. ... 7 Agriculture 15 2011-01-01 2011-01-01 false Environmental requirements. 3550.5 Section...

  19. 7 CFR 3550.5 - Environmental requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... accordance with the requirements provided in 7 CFR part 1940, subpart G which addresses environmental requirements and 7 CFR part 1924, subpart A, which addresses lead-based paint. ... 7 Agriculture 15 2013-01-01 2013-01-01 false Environmental requirements. 3550.5 Section...

  20. 8-Methyl-2-oxo-4-(thio­phen-2-yl)-1,2,5,6,7,8-hexa­hydro­quinoline-3-carbonitrile

    PubMed Central

    Asiri, Abdullah M.; Faidallah, Hassan M.; Saqer, Alaa Anwar Ahmad; Ng, Seik Weng; Tiekink, Edward R. T.

    2012-01-01

    In the title compound, C15H14N2OS, the pyridinone ring in the fused-ring system is nearly planar (r.m.s. deviation = 0.011 Å) and the cyclo­hexene ring has a twisted half-boat conformation with the methyl­ene C atom adjacent to the methine C atom deviating by 0.592 (7) Å from the plane defined by the remaining five atoms (r.m.s. deviation = 0.108 Å). The thienyl ring is disordered over two almost coplanar positions of opposite orientation in a 0.649 (4):0.351 (4) ratio, and forms dihedral angles of 51.4 (3) (major component) and 54.2 (3)°, respectively, with the pyridinone ring. In the crystal, inversion-related mol­ecules associate via an eight-membered {⋯HNCO}2 synthon and these are linked into a linear supra­molecular chain along the a axis by weak π–π inter­actions that occur between centrosymmetrically related pyridinone rings [centroid–centroid distance = 3.889 (2) Å]. PMID:22798934

  1. 7 CFR 657.4 - NRCS responsibilities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    .... (see 7 CFR 600.2(c), 600.6) (4) Coordinate soil mapping units that qualify as prime farmlands with adjacent States, including Major Land Resource Area Offices (see 7 CFR 600.4, 600.7) responsible for the... Conservationists and Major Land Resource Area Offices in inventorying prime and unique farmlands (see 7 CFR...

  2. 7 CFR 657.4 - NRCS responsibilities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    .... (see 7 CFR 600.2(c), 600.6) (4) Coordinate soil mapping units that qualify as prime farmlands with adjacent States, including Major Land Resource Area Offices (see 7 CFR 600.4, 600.7) responsible for the... Conservationists and Major Land Resource Area Offices in inventorying prime and unique farmlands (see 7 CFR...

  3. 7 CFR 657.4 - NRCS responsibilities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    .... (see 7 CFR 600.2(c), 600.6) (4) Coordinate soil mapping units that qualify as prime farmlands with adjacent States, including Major Land Resource Area Offices (see 7 CFR 600.4, 600.7) responsible for the... Conservationists and Major Land Resource Area Offices in inventorying prime and unique farmlands (see 7 CFR...

  4. 7 CFR 657.4 - NRCS responsibilities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    .... (see 7 CFR 600.2(c), 600.6) (4) Coordinate soil mapping units that qualify as prime farmlands with adjacent States, including Major Land Resource Area Offices (see 7 CFR 600.4, 600.7) responsible for the... Conservationists and Major Land Resource Area Offices in inventorying prime and unique farmlands (see 7 CFR...

  5. 7 CFR 657.4 - NRCS responsibilities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    .... (see 7 CFR 600.2(c), 600.6) (4) Coordinate soil mapping units that qualify as prime farmlands with adjacent States, including Major Land Resource Area Offices (see 7 CFR 600.4, 600.7) responsible for the... Conservationists and Major Land Resource Area Offices in inventorying prime and unique farmlands (see 7 CFR...

  6. 7 CFR 550.5 - Competition.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 6 2011-01-01 2011-01-01 false Competition. 550.5 Section 550.5 Agriculture... Competition. REE agencies may enter into non-assistance cooperative agreements, as authorized by this part, without regard to any requirements for competition. (7 U.S.C. 3318(e))....

  7. 7 CFR 550.5 - Competition.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 6 2013-01-01 2013-01-01 false Competition. 550.5 Section 550.5 Agriculture... Competition. REE agencies may enter into non-assistance cooperative agreements, as authorized by this part, without regard to any requirements for competition. (7 U.S.C. 3318(e))....

  8. 7 CFR 550.5 - Competition.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 6 2012-01-01 2012-01-01 false Competition. 550.5 Section 550.5 Agriculture... Competition. REE agencies may enter into non-assistance cooperative agreements, as authorized by this part, without regard to any requirements for competition. (7 U.S.C. 3318(e))....

  9. 7 CFR 550.5 - Competition.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 6 2010-01-01 2010-01-01 false Competition. 550.5 Section 550.5 Agriculture... Competition. REE agencies may enter into non-assistance cooperative agreements, as authorized by this part, without regard to any requirements for competition. (7 U.S.C. 3318(e))....

  10. 7 CFR 550.5 - Competition.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 6 2014-01-01 2014-01-01 false Competition. 550.5 Section 550.5 Agriculture... Competition. REE agencies may enter into non-assistance cooperative agreements, as authorized by this part, without regard to any requirements for competition. (7 U.S.C. 3318(e))....

  11. 41 CFR 51-5.7 - Payments.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 1 2010-07-01 2010-07-01 true Payments. 51-5.7 Section 51-5.7 Public Contracts and Property Management Other Provisions Relating to Public Contracts... Payments. Payments for products or services of persons who are blind or have other severe...

  12. 41 CFR 51-5.7 - Payments.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 41 Public Contracts and Property Management 1 2011-07-01 2009-07-01 true Payments. 51-5.7 Section 51-5.7 Public Contracts and Property Management Other Provisions Relating to Public Contracts... Payments. Payments for products or services of persons who are blind or have other severe...

  13. 41 CFR 51-5.7 - Payments.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 41 Public Contracts and Property Management 1 2012-07-01 2009-07-01 true Payments. 51-5.7 Section 51-5.7 Public Contracts and Property Management Other Provisions Relating to Public Contracts... Payments. Payments for products or services of persons who are blind or have other severe...

  14. 7 CFR 301.74-5 - Compensation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 5 2013-01-01 2013-01-01 false Compensation. 301.74-5 Section 301.74-5 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE DOMESTIC QUARANTINE NOTICES Plum Pox § 301.74-5 Compensation. (a) Eligibility. The following individuals are eligible...

  15. 7 CFR 301.74-5 - Compensation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 5 2014-01-01 2014-01-01 false Compensation. 301.74-5 Section 301.74-5 Agriculture Regulations of the Department of Agriculture (Continued) ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE DOMESTIC QUARANTINE NOTICES Plum Pox § 301.74-5 Compensation. (a) Eligibility. The following individuals are eligible...

  16. 7 CFR 1131.5 - Distributing plant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Distributing plant. 1131.5 Section 1131.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1131.5 Distributing plant. See § 1000.5....

  17. 7 CFR 1006.5 - Distributing plant.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Distributing plant. 1006.5 Section 1006.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1006.5 Distributing plant. See § 1000.5....

  18. 7 CFR 1032.5 - Distributing plant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Distributing plant. 1032.5 Section 1032.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1032.5 Distributing plant. See § 1000.5....

  19. 7 CFR 1005.5 - Distributing plant.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Distributing plant. 1005.5 Section 1005.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1005.5 Distributing plant. See § 1000.5....

  20. 7 CFR 1006.5 - Distributing plant.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Distributing plant. 1006.5 Section 1006.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1006.5 Distributing plant. See § 1000.5....

  1. 7 CFR 1033.5 - Distributing plant.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Distributing plant. 1033.5 Section 1033.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1033.5 Distributing plant. See § 1000.5....

  2. 7 CFR 1124.5 - Distributing plant.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Distributing plant. 1124.5 Section 1124.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Regulating Handling Definitions § 1124.5 Distributing plant. See § 1000.5....

  3. 7 CFR 1124.5 - Distributing plant.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Distributing plant. 1124.5 Section 1124.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Regulating Handling Definitions § 1124.5 Distributing plant. See § 1000.5....

  4. 7 CFR 1126.5 - Distributing plant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Distributing plant. 1126.5 Section 1126.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1126.5 Distributing plant. See § 1000.5....

  5. 7 CFR 1124.5 - Distributing plant.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Distributing plant. 1124.5 Section 1124.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Regulating Handling Definitions § 1124.5 Distributing plant. See § 1000.5....

  6. 7 CFR 1007.5 - Distributing plant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Distributing plant. 1007.5 Section 1007.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1007.5 Distributing plant. See § 1000.5....

  7. 7 CFR 1006.5 - Distributing plant.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Distributing plant. 1006.5 Section 1006.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1006.5 Distributing plant. See § 1000.5....

  8. 7 CFR 1033.5 - Distributing plant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Distributing plant. 1033.5 Section 1033.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1033.5 Distributing plant. See § 1000.5....

  9. 7 CFR 1033.5 - Distributing plant.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Distributing plant. 1033.5 Section 1033.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1033.5 Distributing plant. See § 1000.5....

  10. 7 CFR 1032.5 - Distributing plant.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Distributing plant. 1032.5 Section 1032.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1032.5 Distributing plant. See § 1000.5....

  11. 7 CFR 1131.5 - Distributing plant.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Distributing plant. 1131.5 Section 1131.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1131.5 Distributing plant. See § 1000.5....

  12. 7 CFR 1005.5 - Distributing plant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Distributing plant. 1005.5 Section 1005.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1005.5 Distributing plant. See § 1000.5....

  13. 7 CFR 1033.5 - Distributing plant.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Distributing plant. 1033.5 Section 1033.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1033.5 Distributing plant. See § 1000.5....

  14. 7 CFR 1124.5 - Distributing plant.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Distributing plant. 1124.5 Section 1124.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Regulating Handling Definitions § 1124.5 Distributing plant. See § 1000.5....

  15. 7 CFR 1001.5 - Distributing plant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Distributing plant. 1001.5 Section 1001.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1001.5 Distributing plant. See § 1000.5....

  16. 7 CFR 1005.5 - Distributing plant.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Distributing plant. 1005.5 Section 1005.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1005.5 Distributing plant. See § 1000.5....

  17. 7 CFR 1126.5 - Distributing plant.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Distributing plant. 1126.5 Section 1126.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1126.5 Distributing plant. See § 1000.5....

  18. 7 CFR 1001.5 - Distributing plant.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Distributing plant. 1001.5 Section 1001.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1001.5 Distributing plant. See § 1000.5....

  19. 7 CFR 1030.5 - Distributing plant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Distributing plant. 1030.5 Section 1030.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1030.5 Distributing plant. See § 1000.5....

  20. 7 CFR 1033.5 - Distributing plant.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Distributing plant. 1033.5 Section 1033.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1033.5 Distributing plant. See § 1000.5....

  1. 7 CFR 1032.5 - Distributing plant.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Distributing plant. 1032.5 Section 1032.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1032.5 Distributing plant. See § 1000.5....

  2. 7 CFR 1001.5 - Distributing plant.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Distributing plant. 1001.5 Section 1001.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1001.5 Distributing plant. See § 1000.5....

  3. 7 CFR 1131.5 - Distributing plant.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Distributing plant. 1131.5 Section 1131.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1131.5 Distributing plant. See § 1000.5....

  4. 7 CFR 1032.5 - Distributing plant.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Distributing plant. 1032.5 Section 1032.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1032.5 Distributing plant. See § 1000.5....

  5. 7 CFR 1001.5 - Distributing plant.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Distributing plant. 1001.5 Section 1001.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1001.5 Distributing plant. See § 1000.5....

  6. 7 CFR 1126.5 - Distributing plant.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Distributing plant. 1126.5 Section 1126.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1126.5 Distributing plant. See § 1000.5....

  7. 7 CFR 1131.5 - Distributing plant.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Distributing plant. 1131.5 Section 1131.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1131.5 Distributing plant. See § 1000.5....

  8. 7 CFR 1131.5 - Distributing plant.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Distributing plant. 1131.5 Section 1131.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1131.5 Distributing plant. See § 1000.5....

  9. 7 CFR 1007.5 - Distributing plant.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Distributing plant. 1007.5 Section 1007.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1007.5 Distributing plant. See § 1000.5....

  10. 7 CFR 1030.5 - Distributing plant.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Distributing plant. 1030.5 Section 1030.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1030.5 Distributing plant. See § 1000.5....

  11. 7 CFR 1030.5 - Distributing plant.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Distributing plant. 1030.5 Section 1030.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1030.5 Distributing plant. See § 1000.5....

  12. 7 CFR 1001.5 - Distributing plant.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Distributing plant. 1001.5 Section 1001.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1001.5 Distributing plant. See § 1000.5....

  13. 7 CFR 1126.5 - Distributing plant.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Distributing plant. 1126.5 Section 1126.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1126.5 Distributing plant. See § 1000.5....

  14. 7 CFR 1032.5 - Distributing plant.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Distributing plant. 1032.5 Section 1032.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1032.5 Distributing plant. See § 1000.5....

  15. 7 CFR 1007.5 - Distributing plant.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Distributing plant. 1007.5 Section 1007.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1007.5 Distributing plant. See § 1000.5....

  16. 7 CFR 1126.5 - Distributing plant.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Distributing plant. 1126.5 Section 1126.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1126.5 Distributing plant. See § 1000.5....

  17. 7 CFR 1005.5 - Distributing plant.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Distributing plant. 1005.5 Section 1005.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1005.5 Distributing plant. See § 1000.5....

  18. 7 CFR 1124.5 - Distributing plant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Distributing plant. 1124.5 Section 1124.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Regulating Handling Definitions § 1124.5 Distributing plant. See § 1000.5....

  19. 7 CFR 1030.5 - Distributing plant.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Distributing plant. 1030.5 Section 1030.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1030.5 Distributing plant. See § 1000.5....

  20. 7 CFR 1030.5 - Distributing plant.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Distributing plant. 1030.5 Section 1030.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1030.5 Distributing plant. See § 1000.5....

  1. 7 CFR 1006.5 - Distributing plant.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 9 2011-01-01 2011-01-01 false Distributing plant. 1006.5 Section 1006.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1006.5 Distributing plant. See § 1000.5....

  2. 7 CFR 1007.5 - Distributing plant.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Distributing plant. 1007.5 Section 1007.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1007.5 Distributing plant. See § 1000.5....

  3. 7 CFR 1005.5 - Distributing plant.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Distributing plant. 1005.5 Section 1005.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS... Handling Definitions § 1005.5 Distributing plant. See § 1000.5....

  4. 7 CFR 1006.5 - Distributing plant.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Distributing plant. 1006.5 Section 1006.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1006.5 Distributing plant. See § 1000.5....

  5. 7 CFR 1007.5 - Distributing plant.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Distributing plant. 1007.5 Section 1007.5 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements... Handling Definitions § 1007.5 Distributing plant. See § 1000.5....

  6. Chemistry of the hexaborane(10) analogue (PPh{sub 3}){sub 2}(CO)IrB{sub 5}H{sub 8}: Formation and characterization of the heterobimetallaheptaboranes 1,1,1-(CO){sub 3}-2,2-(CO){sub 2}-2,4-(PPh{sub 3}){sub 2-closo-}-1,2-FeIrB{sub 5}H{sub 4} and 2-(CO)-2,2-(PPh{sub 3}){sub 2}-7-Cl-7-(PMe{sub 2}Ph)-nido-2,7-IrPtB{sub 5}H{sub 7}

    SciTech Connect

    Bould, J.; Rath, N.P.; Fang, H.; Barton, L.

    1996-03-27

    The chemistry of the hexaborane(10) analogue (PPh{sub 3}){sub 2}(CO)IrB{sub 5}H{sub 8} (4) has been investigated. The two standard reactions in which metal-containing moieties may be incorporated into the B{sub 6}H{sub 10} cage find success when applied to the iridahexaborane (4). The reaction of Fe{sub 2}(CO){sub 9} with (PPh{sub 3}){sub 2}(CO)IrB{sub 5}H{sub 8} in C{sub 6}H{sub 6} solution affords 1,1,1-(CO){sub 3}-2,2-(CO){sub 2}-2,4-(PPh{sub 3}){sub 2-closo}-1,2-FeIrB{sub 5}H{sub 4} (1) in moderate (28.5%) yield. Except for carborane derivatives, 1 is the first pentagonal bipyramidal metallaheptaborane to be structurally characterized. Compound 1 was identified by NMR, IR, and high-resolution mass spectroscopy and by a single-crystal X-ray diffraction study. Treatment of a THF solution of the sodium anion salt of 4, Na[(PPh{sub 3}){sub 2}(CO)IrB{sub 5}H{sub 7}], with [Pt(PMe{sub 2}Ph)Cl{sub 2}]{sub 2} in Ch{sub 2}Cl{sub 2} affords yellow solid 2-(CO)-2,2-(PPh{sub 3}){sub 2}-7-Cl-7-(PM3{sub 2}-Ph)-nido-2,7-IrPtB{sub 5}H{sub 7} (2) in 5% yield. 2 is identified similarily. It exists as a nido seven-vertex cluster formally derived by removal of a vertex of connectivity 5 from a closed triangulated dodecahedral polyhedron. 2 is two skeletal electrons short of the electron count for a classical nido cluster, and it is compared to systems with related structures and also to other systems containing the Pt moiety functioning as a two-orbital two-electon donor.

  7. 7 CFR 772.4 - Environmental requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 7 2014-01-01 2014-01-01 false Environmental requirements. 772.4 Section 772.4... AGRICULTURE SPECIAL PROGRAMS SERVICING MINOR PROGRAM LOANS § 772.4 Environmental requirements. Servicing... of security will be reviewed for compliance with 7 CFR part 1940, subpart G and the exhibits to...

  8. 7 CFR 772.4 - Environmental requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 7 2012-01-01 2012-01-01 false Environmental requirements. 772.4 Section 772.4... AGRICULTURE SPECIAL PROGRAMS SERVICING MINOR PROGRAM LOANS § 772.4 Environmental requirements. Servicing... of security will be reviewed for compliance with 7 CFR part 1940, subpart G and the exhibits to...

  9. 42 CFR 4.7 - Fees.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Fees. 4.7 Section 4.7 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS NATIONAL LIBRARY OF MEDICINE § 4.7 Fees. The Director may charge reasonable fees for any service provided by the Library under this...

  10. 42 CFR 4.7 - Fees.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 1 2013-10-01 2013-10-01 false Fees. 4.7 Section 4.7 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS NATIONAL LIBRARY OF MEDICINE § 4.7 Fees. The Director may charge reasonable fees for any service provided by the Library under this...

  11. 42 CFR 4.7 - Fees.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Fees. 4.7 Section 4.7 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS NATIONAL LIBRARY OF MEDICINE § 4.7 Fees. The Director may charge reasonable fees for any service provided by the Library under this...

  12. 42 CFR 4.7 - Fees.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false Fees. 4.7 Section 4.7 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS NATIONAL LIBRARY OF MEDICINE § 4.7 Fees. The Director may charge reasonable fees for any service provided by the Library under this...

  13. 42 CFR 4.7 - Fees.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false Fees. 4.7 Section 4.7 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GENERAL PROVISIONS NATIONAL LIBRARY OF MEDICINE § 4.7 Fees. The Director may charge reasonable fees for any service provided by the Library under this...

  14. 49 CFR 7.4 - Publication required.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false Publication required. 7.4 Section 7.4 Transportation Office of the Secretary of Transportation PUBLIC AVAILABILITY OF INFORMATION Information Required To Be Made Public by DOT § 7.4 Publication required. (a) General. The material described in §...

  15. 49 CFR 7.4 - Publication required.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 1 2012-10-01 2012-10-01 false Publication required. 7.4 Section 7.4 Transportation Office of the Secretary of Transportation PUBLIC AVAILABILITY OF INFORMATION Information Required To Be Made Public by DOT § 7.4 Publication required. (a) General. The material described in §...

  16. 49 CFR 7.4 - Publication required.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 1 2013-10-01 2013-10-01 false Publication required. 7.4 Section 7.4 Transportation Office of the Secretary of Transportation PUBLIC AVAILABILITY OF INFORMATION Information Required To Be Made Public by DOT § 7.4 Publication required. (a) General. The material described in §...

  17. 49 CFR 7.4 - Publication required.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Publication required. 7.4 Section 7.4 Transportation Office of the Secretary of Transportation PUBLIC AVAILABILITY OF INFORMATION Information Required To Be Made Public by DOT § 7.4 Publication required. (a) General. The material described in §...

  18. 30 CFR 7.4 - Product testing.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Product testing. 7.4 Section 7.4 Mineral... MINING PRODUCTS TESTING BY APPLICANT OR THIRD PARTY General § 7.4 Product testing. (a) All products... to observe product testing, the applicant shall permit an MSHA official to be present at a...

  19. Synthesis, characterisation, conformational preferences, dynamic NMR studies and antimicrobial evaluation of N-nitroso- and N-formyl-c-3,t-3-dimethyl-r-2,c-7-diphenyl-1,4-diazepan-5-ones

    NASA Astrophysics Data System (ADS)

    Ponnuswamy, S.; Akila, A.; Kiruthiga devi, D.; Maheshwaran, V.; Ponnuswamy, M. N.

    2016-04-01

    The stereochemical preferences of N-nitroso- and N-formyl-c-3,t-3-dimethyl-r-2,c-7-diphenyl-1,4-diazepan-5-ones 3 & 4, respectively, have been determined using 1D and 2D NMR spectral techniques. Interestingly, the N-nitroso compound 3 is found to prefer an equilibrium between alternate chair conformations with diaxial phenyl groups, while the N-formyl compound 4 prefers flattened boat conformation. This is stereochemically a novel report on the flexible rings adopting a chair conformation with diaxial phenyl groups. The X-ray crystal structure of N-nitroso-c-3,t-3-dimethyl-r-2,c-7-diphenyl-1,4-diazepan-5-one (3) also supports the chair conformation with diaxial phenyl groups. Dynamic 1H NMR spectral studies have been carried out on the N-nitroso and N-formyl diazepan-5-ones 3 &4 and the energy barriers for N-NO and N-CO rotations are found to be 88.7 and 75.7 kJ/mol, respectively. The antimicrobial activities have been determined for the compounds 2-4 and it is found that all the compounds exhibit significant antibacterial and antifungal activities when compared to the standard chloramphenicol.

  20. Synthesis, characterization, biological evaluation and docking studies of 2‧-[(2″,4″-difluorobiphenyl-4-yl)carbonyl]-1‧-aryl-1‧,2‧,5‧,6‧,7‧,7a‧-hexahydrospiro[indole-3,3‧-pyrrolizin]-2(1H)-ones

    NASA Astrophysics Data System (ADS)

    Fathimunnisa, M.; Manikandan, H.; Neelakandan, K.; Rajendra Prasad, N.; Selvanayagam, S.; Sridhar, B.

    2016-10-01

    A series of novel 2‧-[(2″,4″-difluorobiphenyl-4-yl)carbonyl]-1‧-aryl-1‧,2‧,5‧,6‧,7‧,7a‧-hexahydrospiro[indole-3,3‧-pyrrolizin]-2(1H)-ones were regioselectively synthesized by multicomponent reaction with excellent yield. They were characterized by elemental analysis and various spectral techniques. Sensing ability of the compound to different metal ions was investigated. To ascertain the inhibitory activity of the compounds they were subjected to in vitro antimicrobial studies against various microbes and cytotoxic studies against Hep-2 cell line. The ligand-receptor interaction of the compounds was explored by molecular docking and the human folate receptor (4LRH) was identified as the potential target protein. DFT calculations were incorporated in the study to compare the molecular structure and geometrical parameters of 2‧-[(2″,4″-difluorobiphenyl-4-yl)carbonyl]-1‧-phenyl-1‧,2‧,5‧,6‧,7‧,7a‧-hexahydrospiro[indole-3,3‧-pyrrolizin]-2(1H)-one with experimental data.