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Sample records for 4-pyridinedicarbonitrile 3-aminophthalonitrile 4-aminophthalonitrile

  1. Biotransformation of heterocyclic dinitriles by Rhodococcus erythropolis and fungal nitrilases.

    PubMed

    Vejvoda, Vojtech; Sveda, Ondrej; Kaplan, Ondrej; Prikrylová, Vera; Elisáková, Veronika; Himl, Michal; Kubác, David; Pelantová, Helena; Kuzma, Marek; Kren, Vladimír; Martínková, Ludmila

    2007-07-01

    2,6-Pyridinedicarbonitrile (1a) and 2,4-pyridinedicarbonitrile (2a) were hydrated by Rhodococcus erythropolis A4 to 6-cyanopyridine-2-carboxamide (1b; 83% yield) and 2-cyanopyridine-4-carboxamide (2b; 97% yield), respectively, after 10 min. After 118 h, the intermediates 1b or 2b were transformed into 2,6-pyridinedicarboxamide (1c; 35% yield) and 2,6-pyridinedicarboxylic acid (1d; 60% yield) or 2-cyanopyridine-4-carboxylic acid (2c; 64% yield), respectively. The nitrilase from Fusarium solani afforded cyanocarboxylic acids 1e and 2c after 118 h (yields 95 and 62%, respectively). 3,4-Pyridinedicarbonitrile (3a) and 2,3-pyrazinedicarbonitrile (4a) were inferior substrates of nitrile hydratase and nitrilase.